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Sample records for mucosa del intestino

  1. Relación endoscópica entre duodenitis inespecífica y sobrecrecimiento bacteriano del intestino delgado

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    Naddaf, R; Folkmanas, W

    2011-01-01

    Cuando aparece una proliferación de la flora de tipo colónico en el intestino delgado se producen alteraciones en la digestión y absorción intestinal, que clínicamente conforman el síndrome del Sobrecrecimiento Bacteriano del Intestino Delgado (SBID). En el SBID la concentración bacteriana aumenta hasta 107-109 UFC/ml en intestino delgado. El principio del test de hidrógeno espirado es la administración de un carbohidrato (Lactulosa, dxilosa, glucosa), que al ser degradado por las bacterias p...

  2. El intestino: pieza clave del sistema inmunitario The bowel: A key component of the immune system

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    E. Ramiro-Puig

    2008-01-01

    Full Text Available El intestino se halla expuesto constantemente a una elevada carga antigénica procedente de la dieta y de bacterias comensales. El tejido linfoide asociado al intestino (Gut-Associated Lymphoid Tissue, GALT constituye la parte más extensa y compleja del sistema inmunitario y es capaz de discriminar de forma eficaz entre patógenos invasivos y antígenos inocuos. El conocimiento de su particular subdivisión en tejido organizado, inductor de la respuesta inmunitaria (placas de Peyer y ganglios linfáticos mesentéricos, y tejido difuso, efector de la respuesta inmunitaria (linfocitos intraepiteliales y linfocitos de lámina propia, nos permite comprender cómo se desarrolla y regula la respuesta inmunitaria en el intestino y como esta puede extenderse al resto del organismo.The gut is constantly exposed to a high antigenic load coming from the diet and commensal bacteria. The Gut-Associated Lymphoid Tissue (GALT constitutes the most extensive and complex part of the immune system and is capable of efficiently distinguishing invasive pathogens from innocuous antigens. The knowledge of its unique structure consisting on organised tissue, inductor of the immune response (Peyer's patches and mesenteric lymph nodes, and diffused tissue, effector of the immune response (intraepithelial lymphocytes and lamina propria lymphocytes, allow us to understand the development and regulation of the immune response in the gut and how this one can be extended to the rest of the organism.

  3. Conflictos cognitivos en el Síndrome del Intestino Irritable (SII: un estudio exploratorio

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    Ruth Benasayag

    2004-01-01

    Full Text Available En este estudio descriptivo transversal se exploran algunas características cognitivas y sintomáticas de 13 pacientes consultantes con Síndrome del Intestino Irritable (SII comparados con una muestra de 63 sujetos no consultantes sin síntomas del SII ni psicopatológicos. El 92% de los primeros cumple criterios para algún trastorno del eje I del DSM-IV y su diagnóstico principal puede agruparse en dos categorías: trastornos por ansiedad y por somatización. En conjunto, los pacientes con SII muestran más conflictos cognitivos detectados mediante la Técnica de Rejilla (TR de Kelly que los sujetos normales. Considerando el subgrupo de trastorno por somatización, se observa una tendencia mucho más acentuada a mostrar mayor número de estos conflictos, así como una tendencia clara a presentar mayor polarización y, a su vez, menor complejidad cognitiva (diferenciación, con respecto tanto a los sujetos asintomáticos como a los diagnosticados por trastorno de ansiedad; por su parte, éstos muestran una menor autoestima.

  4. Irritable bowel syndrome immune hypothesis: the role of lymphocytes and mast cells Hipótesis inmune del síndrome del intestino irritable: Primera parte: papel de los linfocitos y mastocitos

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    M. Ortiz Lucas

    2010-11-01

    Full Text Available Objective: To review the available evidence on the role of T-lymphocytes and mast cells in the etiopathogenesis of Irritable Bowel Syndrome. Methods: Bibliographic retrieval on PubMed including the terms "Irritable Bowel Syndrome, "Immune System", "T-Lymphocytes" and "Mast Cells". Results: Twenty-five case-control studies and one randomized controlled trial were retrieved. Noteworthy in the blood is the increase in activated T cells destined to migrate to the bowel in these patients. A high frequency of T-lymphocytes is described in the intestinal mucosa, although the study findings are, at times, contradictory. An evident increase in mast cells (and in their activity between the terminal ileum and descending colon is also observed. Conclusions: The heterogeneity of diagnostic criteria and experimentation methods could account for some of the differences in the results found in the selected research. There are indications that give reason to believe these patients have "low-grade intestinal inflammation", and the increase in T-lymphocytes and mast cells has been associated with disorders found in IBS such as the communication between the intestine and the nervous system, the increase in intestinal permeability and changes in the microbiota.Objetivo: Revisar la evidencia disponible sobre el papel de los linfocitos T y mastocitos en la etiopatogenia del Síndrome del Intestino Irritable. de las vías biliares. Métodos: Recuperación bibliográfica en PubMed incluyendo los términos "Irritable Bowel Syndrome, "Immune System", "T-Lymphocytes" y "Mast Cells". Resultados: Se recuperaron 25 estudios casos-control y un ensayo clínico aleatorizado. A nivel sanguíneo destaca el aumento de células T activadas destinadas a migrar al intestino en estos pacientes. En la mucosa intestinal se describe un patrón elevado de linfocitos T, aunque los resultados de los estudios son en ocasiones contradictorios, y un aumento claro de mastocitos (y de su

  5. Evaluación del efecto de Lactobacillus spp. en el desarrollo del intestino delgado en pollos de engorde

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    Sandra Paola Rodríguez-González

    2016-01-01

    Full Text Available Los probióticos del tipo Lactobacillus spp. son considerados alternativas promisorias para reemplazar los antibióticos como promotores de crecimiento (APC en las explotaciones avícolas, ya que influyen en el desarrollo poseclosión del tracto gastrointestinal (TGI y en su ecología microbiana. En este estudio se evaluó la morfometría del intestino delgado en pollos de engorde al suministrar 0.5mL/L de un producto comercial a base de Lactobacillus spp. Se utilizaron 200 pollos machos de un día de edad, de la línea Avian Cobb 48, los cuales se distribuyeron, con un diseño completamente al azar, en dos grupos, denominados control y experimento; ambos grupos fueron mantenidos en condiciones similares de alimentación, manejo y sanidad. A los días 7, 15 y 35 de edad se realizó el sacrificio y la necropsia; se disectaron segmentos de duodeno, yeyuno e íleon para su procesamiento con la técnica de coloración de Hematoxilina-Eosina (HE. Los cambios morfométricos de longitud, ancho y cantidad de vellosidades fueron evaluados utilizando un microscopio trinocular con cámara digital Moticam®. Los datos se analizaron usando el programa SPSS versión 19; se realizaron comparaciones de los promedios a través de estadística descriptiva y una prueba ANOVA (p<0,05; se observaron diferencias estimadas significativas (p<0,05 a favor del grupo experimento en el desarrollo poseclosión de los segmentos yeyuno e íleon.

  6. Comparison of three contrast radiographic techniques in the dog large intestine; Comparacion de tres tecnicas radiograficas de contraste en el intestino grueso del perro

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    Vargas, L.; Thibaut, J.; Olhaberry, E.; Born, R.; Deppe, R. [Universidad Austral de Chile, Valdivia (Chile)

    1994-07-01

    In order to compare three radiographic techniques -pneumocolon, barium enema and double contrast- in the large intestine of the dog, three radiographic series in ventrodorsal and right lateral projections were taken. Six healthy adult dogs of both sexes with an approximate weight between 5 to 10 kg were used. Three enemas were administered 24, 12 and 2 hrs. before the series of radiographs were taken. Then dogs were anaesthetized with sodium tiopental (20 mg/kg iv) and the contrast media were introduced. Pneumocolon was carried out in the first series introducing air (20 cc/kg) in the large intestine through a Foley rectal catheter. Radiographs were taken in both projections, after 5 and 15min. respectively. Barium enema was performed in the second series introducing barium sulfate (18%) in the large intestine through a Foley rectal catheter (25 cc/kg); 5 and 15 min. later, the radiographs were taken. In the third series -double contrast- the barium sulfate, which was obtained from each dog using a catheter, was substituted by a volume of air equal to that obtained from the contrast medium. Later the radiographs were taken in both projections. The radiographic plates of each series were analized comparing the characteristics of: radiographic density, outline and volume. With the pneumocolon barium enema and double contrast, the radiographic density was, in most cases, low, high and inter-mediate respectively. The radiographic outline was, in most cases, regular for the three techniques. Thee radiographic volume was similar in all of the series. From the results obtained, it is concluded that double contrast best outlines the intestinal mucosa and more information can be obtained from it [Spanish] Con la finalidad de comparar tres tecnicas radiograficas del intestino grueso del perro -neumocolon, enema baritado y doble contraste-, se tomaron tres series radiograficas en proyeccion ventrodorsal y lateral derecha en 6 perros adultos, de 5 a 10 kg de peso, que

  7. Síndrome de intestino irritable y diverticulosis

    OpenAIRE

    Kestenberg Himelfar, Abraham; Fundación Valle de Lili

    1998-01-01

    Síndrome de intestino irritable (SII)/ ¿Cómo funciona el sistema digestivo?/¿Qué es el síndrome de intestino irritable?/¿Cuáles son los síntomas del síndrome de intestino irritable?/¿Qué causa el síndrome de intestino irritable?/¿Qué papel juega la tensión emocional en el origen del síndrome de intestino irritable?/¿Cómo saber si el problema es síndrome de intestino irritable o si es algo diferente?/¿Cómo se debe tratar el síndrome de intestino irritable?/¿Se deben evitar algunos alimentos?/¿...

  8. Viabilidade celular da mucosa do intestino delgado de ratos, após correção de choque hipovolêmico com solução de NaCl 7,5%

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    Brito Marcus Vinicius Henriques

    2003-01-01

    Full Text Available OBJETIVO: Estudar o efeito da correção volêmica com diferentes tipos de solução, na mucosa do intestino delgado de ratos. MÉTODOS: Foram utilizados 120 ratos Wistar (Rattus norvegicus albinus, machos, adultos, com peso individual entre 310 e 410g, oriundos do Instituto Evandro Chagas de Belém do Pará, submetidos a período de adaptação por 15 dias, recebendo água e ração ad libitum, durante todo o experimento. Os animais foram distribuídos em: Grupo Padrão (P, Grupo Choque (C, Grupo Solução Fisiológica (SF e Grupo Solução Hipertônica (SH, com 30 animais cada. Estes foram divididos em subgrupos com 10 animais cada, de acordo com o dia de pós-operatório (DPO previsto para a eutanásia dos animais, (1masculine, 3masculine ou 7masculine DPO, sendo após esta, colhido material para realização de teste de absorvância pelo MTT em todos os animais. RESULTADOS: O grupo SF apresentou menores índices de viabilidade celular comparado aos grupos SH e C (p<0.05. CONCLUSÃO: A correção volêmica com solução de cloreto de sódio a 7.5% levou a manutenção de maior quantidade de células viáveis, no intestino delgado em ratos no 7masculine dia do experimento.

  9. Estudo morfométrico e estereológico digital da mucosa do intestino delgado de crianças eutróficas e desnutridas com diarréia persistente

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    Pires Ana L.G.

    2003-01-01

    Full Text Available OBJETIVOS: testar a hipótese de que a mucosa do intestino delgado proximal de crianças com diarréia persistente apresenta alterações morfométricas e estereológicas proporcionais ao estado nutricional, utilizando captura de imagens através de microscópio acoplado ao computador. MÉTODOS: estudo transversal incluindo 65 pacientes pediátricos, com idade entre quatro meses e cinco anos, com diarréia de mais de 14 dias de duração, avaliados pelos escores z, divididos em eutróficos = z > 2 DP e desnutridos = z 2 DP, risco nutricional = z < -1DP e desnutridos = z < -2DP; e de maneira contínua em ordem decrescente, utilizando-se as tabelas do NCHS. Após a captura das imagens através do programa Scion Image, foram medidas a altura dos vilos, a profundidade das criptas, a espessura da mucosa, a espessura total da mucosa e a relação vilo/cripta (100 aumentos e a altura do enterócito, altura do núcleo e do bordo em escova (500 aumentos. A análise estereológica foi feita através de arcos ciclóides. RESULTADOS: para os escores z P/I, P/E e E/I, divididos em duas categorias de estado nutricional, não houve diferença estatisticamente significante quanto às medidas da altura dos vilos, profundidade das criptas, espessura da mucosa, espessura total da mucosa e relação vilo/cripta. A altura do enterócito foi a característica que apresentou maior diferença entre os grupos eutróficos e desnutridos, para os índices P/I e P/E, em 500 aumentos, sem atingir significância estatística. No entanto, para três categorias de estado nutricional, a análise morfométrica digitalizada mostrou diferença estatisticamente significante para a relação vilo/cripta entre eutróficos e desnutridos leves e entre eutróficos e desnutridos moderados e graves (p=0,048. A relação vilo/cripta foi maior nos eutróficos. Através do coeficiente de correlação linear de Spearman, a altura do enterócito, a altura do núcleo do enterócito e a do bordo

  10. Seguimiento multidisciplinario en el control del penfigoide de membranas mucosas

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    Fabiano Jeremias

    2011-06-01

    Full Text Available El término penfigoide de membranas mucosas designa un grupo de enfermedades vesículo-bulosas autoinmunes crónicas, que afectan predominantemente mucosas. El presente artículo describe dos casos clínicos de penfigoide de membranas mucosas con compromiso gingival y tuvo como objetivo resaltar la importancia de un correcto diagnóstico de la lesión y discutir la conducta terapéutica. Fueron realizados diversos exámenes complementares como, examen de Nikolsky, biopsia incisional, investigación de células LE y de anticuerpos antinucleares, además de la evaluación de médicos especializados en dermatología y oftalmología. Fue fundamental la substitución de dentífricos, la orientación nutricional y psicológica, el cuidado con la higiene, además del uso de corticoides tópicos. Hubo periodos de remisión y exacerbación del cuadro clínico durante el seguimiento, fue necesario el ajuste en la terapéutica y refuerzo en los cuidados con la higiene bucal. Es muy importante la interacción multidisciplinaria en la atención de estos casos para el control del tratamiento y seguimiento, además de reforzar todas las orientaciones y cuidados en lo que se refiere a la salud bucal y cautela en el uso de corticoides.

  11. Intestino Corto Short bowel syndrome

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    María Matilde Socarrás Suárez

    2004-06-01

    Full Text Available El intestino corto está asociado a pérdida o disfunción del intestino delgado por resección del mismo, que causa diarreas, tránsito intestinal acelerado, malabsorción intestinal, y eventualmente la pérdida de peso y el desgaste muscular. El objetivo de este trabajo fue actualizar el conocimiento acerca de este síndrome. Se realiza una revisión del tema de intestino corto donde se refiere a su definición, causas fundamentales frecuentes e infrecuentes en el niño y en el adulto, cómo se adapta el intestino a la resección de diferentes extensiones, las funciones del íleon terminal. Se hacen una valoración clínica inicial, con el interrogatorio médico, revisión minuciosa de la historia clínica para cuantificar la capacidad de absorción. Se habla de los síntomas y signos de deficiencia nutricional. Se explican las estrategias del tratamiento, que tienen 3 etapas de evolución clínica. Se concluye que se indica la dietoterapia adecuada según el estado nutricional del paciente y la resección intestinal realizada, evitando las complicaciones para lograr una calidad máxima de vidaShort bowel is associated with loss or dysfunction of the small bowel due to its resection, which causes diarrheas, accelerated intestinal transit, intestinal malabsorption and, eventually, weight loss ansd muscular waste. The objective of this paper was to update knowledge about this syndrome. A review of the short intestine topic is made, making reference to its definition, common and uncommon main cuases in the child and adult, how the bowel adapts itslef to resection of different extensions, and the functions of the terminal ileum. An initial clinical assessment is made with the medical questionnaire and a detailed review of the medical history to quantify the absorption capacity. The symptoms and signs of nutritional deficiency are dealt with. The strategies of the treatment consisting of 3 stages of clinical evolution are explained. It is concluded

  12. CARACTERIZACIÓN DE BACTERIAS PROBIÓTICAS AISLADAS DEL INTESTINO GRUESO DE CERDOS COMO ALTERNATIVA AL USO DE ANTIBIÓTICOS

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    Henry Jurado G

    2009-08-01

    Full Text Available Objetivos. Caracterizar bacterias ácido lácticas probióticas aisladas del intestino grueso de cerdos adultos como posible alternativa al uso de antibióticos. Materiales y métodos. Se incluyeron muestras de intestino grueso de 20 cerdos adultos, para el aislamiento de bacterias lácticas (BAL. La caracterización de los parámetros bioquímicos-fisiológicos de las BAL contemplo la evaluación del pH del fermento, determinación de ácidos orgánicos por HPLC y detección de isómeros de ácido láctico. Los procedimientos microbiológicos evaluaron la capacidad de inhibición del crecimiento de patógenos y la actividad antagónica del probiótico vs antibiótico con su interacción competitiva. Se utilizaron pruebas API CHL 50 para la identificación de las BAL que se complementaron con la producción de gas, actividad de catalasa y viabilidad a diferentes concentraciones de sales biliares y temperaturas. Resultados. El plan de análisis permitió seleccionar dos aislados que se identificaron como: Lactobacillus plantarum 1 H1 y Lb. plantarum 1 H2. Las pruebas microbiológicas evidenciaron que las BAL seleccionadas inhibieron principalmente los enteropatógenos: Escherichia coli, Salmonella typhimurium y Clostridium perfringens. Las pruebas bioquímicas fueron compatibles con el género Lactobacillus, mostrando el siguiente perfil: Catalasa negativas; no productoras de gas; productoras de isómeros DL; resistentes a sales biliares y pH óptimo de crecimiento 3.5 a 38oC. Conclusiones. Los dos aislados de Lactobacillus plantarum 1 reunieron las características para el uso potencial como probióticos. Se recomienda su uso como complemento de terapias con antibióticos y en la elaboración de inóculos para aplicación en raciones alimenticias de lechones destetos, sin embargo, estudios complementarios se requieren para evaluar el efecto directo del probiótico como sustituto del uso de antibióticos in vivo.

  13. Irritable bowel syndrome immune hypothesis: Part two: the role of cytokines Hipótesis inmune del síndrome del intestino irritable: Segunda parte: papel de las citokinas

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    M. Ortiz Lucas

    2010-12-01

    Full Text Available Objective: To review the available evidence on the role of interleukins in the etiopathogenesis of Irritable Bowel Syndrome. Methods: Bibliographic retrieval on PubMed including the MeSH terms "Irritable Bowel Syndrome", "Immune System", "Cytokines" and "Interleukins". Results: Sixteen case-control studies and one randomised controlled trial were retrieved. The blood appears to have a high concentration of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-8 and lower concentration of IL-10, an anti-inflammatory cytokine, even though the findings are disparate and heterogeneous. As many as 33 genes were found, each with different expressions, and a diminished expression of cytokines in the colon mucosa of patients with IBS, which have not been previously described in any other pathology. Conclusions: In patients with IBS, a clear profile of cytokine levels in the blood does not appear to exist, although an imbalance between them can be observed. Moreover, there are indications that give reason to believe that the different subsets of patients with IBS could present cytokine profiles in different blood. On the other hand, in the intestine, high cytokine secretion levels are not detected, contrary to what would be expected. Further studies are required to substantiate these findings.Objetivo: Revisar la evidencia disponible sobre el papel de las interleukinas en la etiopatogenia del Síndrome del Intestino Irritable. Métodos: Recuperación bibliográfica en PubMed, incluyendo los términos MeSH "Irritable Bowel Syndrome", "Immune System", "Cytokines" e "Interleukins". Resultados: Se recuperaron 16 estudios casos-control y un ensayo clínico aleatorizado. A nivel sanguíneo, parece existir una concentración elevada de citokinas proinflamatorias (FNT-α, IL-1β, IL-6, IL-8 y disminuida de la IL-10, una citokina antiinflamatoria, si bien los resultados son dispares y heterogéneos. Se han encontrado hasta 33 genes, cada uno con una expresi

  14. Dos casos de quiste de intestino anterior en cavidad oral

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    Celso Chuquisana-Mostacero

    Full Text Available Antecedentes y Objetivo: El quiste de intestino anterior es una entidad congénita poco frecuente. Cuando aparece, su localización más frecuente es el íleon y, aunque pueden aparecer a lo largo de todo el tracto digestivo, es rara su ubicación en la cavidad oral. A menudo se caracteriza por tres hallazgos principales: presencia de una capa de músculo liso bien desarrollada, una capa epitelial típica de alguna porción del tracto alimentario, y la unión íntima a alguna porción del tracto gastrointestinal. Las lesiones quísticas del suelo de boca en la edad infantil más frecuentes son los quistes salivares y las malformaciones linfáticas, ambas englobables en el término ránula. Sin embargo hay otras posibilidades, y la duplicación de intestino anterior, como aparece en los casos que presentamos, es una de ellas. Material y Método: Presentamos 2 pacientes en edad pediátrica con lesión lingual o de suelo de boca, que tras el estudio de imagen adecuado fueron operados por vía intraoral para la extirpación de la tumoración. En uno de los casos se usó abordaje cervical para extirpación de un segundo locus quístico. Resultados: El estudio anatomopatológico mostró en uno de los casos epitelio respiratorio, y en el segundo mucosa gástrica y epitelio respiratorio. Conclusiones: La importancia del diagnóstico y la extirpación completa en este tipo de lesiones viene dada por el riesgo de degeneración maligna en la edad adulta.

  15. Weight loss and morphometric study of intestinal mucosa in rats after massive intestinal resection: influence of a glutamine-enriched diet Perda de peso e estudo morfométrico da mucosa intestinal de ratos submetidos à ressecção subtotal de intestino delgado: influência do uso de dieta com glutamina

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    Sidney Resende Ribeiro

    2004-01-01

    standard-diet group. CONCLUSIONS: In rats with experimentally produced short-bowel syndrome, glutamine-enrichment of an isonitrogenous test diet was associated with an improved adaptation response by the intestinal mucosa but not reduced weight loss. However, the adaptation response in the group receiving the glutamine-enriched diet was not improved over that for the group fed regular chow.A síndrome do intestino curto é responsável por alterações metabólicas que comprometem o estado nutricional do paciente. A glutamina é nutriente essencial para os enterócitos. OBJETIVOS: Estudou-se o efeito da administração de dieta com glutamina, em ratos submetidos à ressecção subtotal do intestino delgado, avaliando a perda de peso pós-operatória e a morfometria da mucosa intestinal. MÉTODOS: Foram constituídos três grupos de ratos Wistar machos recebendo as seguintes dietas: com glutamina (grupo EG, sem glutamina (grupo EsG, e a dieta padrão do laboratório (grupo ER. A ressecção intestinal foi realizada em todos os animais incluindo-se a válvula íleo-cecal, com remanescente jejunal de apenas 25cm a partir do piloro, anastomosado látero-lateralmente ao cólon ascendente. A diferença entre o peso inicial real e ao final do experimento no 20º dia pós-operatório foi registrada antes do sacrifício. Fragmentos de mucosa do duodeno e jejuno foram colhidos e corados com hematoxilina-eosina. Realizou-se o estudo morfométrico da mucosa intestinal, por meio de sistema digital associado ao microscópio ótico. Mediu-se a altura das vilosidades, a profundidade das criptas e a extensão total da mucosa intestinal. RESULTADOS: A perda de peso entre os três grupos não diferiu estatisticamente. A análise da morfometria da mucosa intestinal mostrou diferença significativa: 1 animais do grupo Enterectomia com Glutamina (EG apresentaram vilosidade duodenal significativamente maior que o grupo EsG (p< 0,05, mas não diferiram do grupo que recebeu dieta padrão do

  16. Guía de práctica clínica: síndrome del intestino irritable con estreñimiento y estreñimiento funcional en adultos: concepto, diagnóstico y continuidad asistencial. (Parte 1 de 2)

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    F. Mearin; C. Ciriza; M. Mínguez; E. Rey; J.J. Mascort; E. Peña; P. Cañones; J. Júdez

    2017-01-01

    En esta Guía de práctica clínica analizamos el manejo diagnóstico y terapéutico de pacientes adultos con estreñimiento y molestias abdominales, bajo el espectro del síndrome del intestino irritable y el estreñimiento funcional. Tienen una importante repercusión personal, sanitaria y social, afectando a la calidad de vida de los pacientes que las padecen. En el síndrome del intestino irritable con predomino del estreñimiento, este es la alteración deposicional predominante junto con dolor abdo...

  17. Enteroscopia de doble balón para el diagnóstico de las enfermedades del intestino delgado en los niños Double-balloon enteroscope for the diagnosis of small intestine diseases in children

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    Héctor Rubén Hernández Garcés

    2010-12-01

    Full Text Available INTRODUCCIÓN. La enteroscopia de doble balón (EDB es un nuevo método que permite la visualización completa de la luz del intestino delgado. La presente investigación se realizó con el objetivo de evaluar la experiencia en la realización de esta técnica en niños atendidos en el Servicio de Endoscopia Pediátrica del Instituto Cubano de Gastroenterología. MÉTODOS. Se realizó un estudio descriptivo retrospectivo para evaluar la seguridad, eficacia y extensión de exploración de la enteroscopia de doble balón en el diagnóstico de las enfermedades del intestino delgado en niños. Para ello fueron estudiados 8 pacientes con examen físico y complementarios negativos de enfermedad del intestino delgado, atendidos entre noviembre de 2008 y octubre de 2009. En 3 de los 8 pacientes existía la sospecha clínica y radiológica de enfermedad de Crohn; en otros 3, la sospecha de tumor de intestino delgado y los restantes fueron atendidos por presentar sangramiento oculto de origen intestinal. RESULTADOS. Se practicaron 7 procedimientos por vía retrógrada y 2 por vía anterógrada; a un paciente se le realizó enteroscopia total empleando ambas vías. La duración media de la EDB por vía oral fue de 62,5 min, con un intervalo de 60 a 65 min y por la vía anal fue de 50,7 min, con intervalo de 45 a 60 min. En todos los casos se logró identificar alguna lesión que explicara los síntomas. CONCLUSIÓN. La enteroscopia de doble balón es un método seguro para el diagnóstico de enfermedades del intestino delgado en los niños.INTRODUCTION. The double balloon enteroscope (DBE is a new method allows the whole visualization of the small intestine lumen. The objective of present research was to assess the experience in the carrying out of this technique in children seen in the Pediatric Endoscopy Service of the Cuban Institute of Gastroenterology. METHODS. A retrospective and descriptive study was conducted to assess the accuracy, effectiveness

  18. Bacterias celulolíticas aisladas del intestino de termitas (Nasutitermes nigriceps con características probióticas y potencial en la degradación del pasto

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    Cecilia Lara Mantilla

    2013-06-01

    Full Text Available Título corto: Bacterias celulolíticas aisladas del intestino de termitas (Nasutitermes nigriceps  Título en ingles:  Cellulolytic bacteria isolated from  termites’ gut (Nasutitermes nigriceps with probiotic characteristics and potential pasture degradationResumen: El objetivo de la presente investigación fue aislar bacterias celulolíticas del intestino de termitas (Nasutitermes nigriceps para determinar sus propiedades probióticas in vitro y su potencial en la degradación de pasto. Las termitas fueron tratadas con detergente antibacterial, separando y macerando luego, el intestino de las mismas en agua peptonada estéril. Diluciones de esta mezcla fueron inoculadas en cajas Petri con medio Luria Bertani (LB y Carboximetilcelulosa (CMC al 2%, incubando a 37º C por 24 horas, para luego revelar con Rojo Congo al 1%. Las bacterias que presentaron mayores halos de degradación fueron sometidas a tinción de Gram y a pruebas probióticas de temperatura, pH, salinidad y presencia de sales biliares, así como también a pruebas de antagonismo y degradación de pasto. Los resultados revelaron la presencia de 9 bacilos celulolíticos Gram (- de los cuales, los bacilos BTN7 y BTN8, presentaron los mejores halos de degradación, 12 y 14 mm de diámetro respectivamente, creciendo adecuadamente en las diferentes pruebas probióticas con densidades entre 106 y 108 UFC/ml; el porcentaje de degradación de materia seca fue de 39.73% y 36.10% en 48 y 72 horas respectivamente.  Las pruebas bioquímicas API E (bioMérieux SA revelaron que los bacilos BTN7 y BTN8 pertenecen al género Enterobacter sp. Los anteriores resultados abren la posibilidad de emplear, estos microorganismos como aditivos en la alimentación de rumiantes, a fin de contribuir con un mayor aprovechamiento de pastos, y otros sustratos vegetales lignocelulósicos.Palabras claves: Probióticos, Maralfalfa (Pennisetum sp., microorganismos ruminales, lignocelulosa, Enterobacter

  19. Guía de práctica clínica del síndrome del intestino irritable con estreñimiento y estreñimiento funcional en adultos: tratamiento. (Parte 2 de 2

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    F. Mearin

    2017-03-01

    Full Text Available En esta Guía de práctica clínica analizamos el manejo diagnóstico y terapéutico de pacientes adultos con estreñimiento y molestias abdominales, bajo la confluencia del espectro del síndrome del intestino irritable y el estreñimiento funcional. Ambas patologías están encuadradas en los trastornos funcionales intestinales y tienen una importante repercusión personal, sanitaria y social, afectando a la calidad de vida de los pacientes que las padecen. La primera es el subtipo de síndrome del intestino irritable en el que el estreñimiento es la alteración deposicional predominante junto con dolor abdominal recurrente, hinchazón y distensión abdominal frecuente. El estreñimiento se caracteriza por la dificultad o la escasa frecuencia en relación con las deposiciones, a menudo acompañado por esfuerzo excesivo durante la defecación o sensación de evacuación incompleta. En la mayoría de los casos no tiene una causa orgánica subyacente, siendo considerado un trastorno funcional intestinal. Son muchas las similitudes clínicas y fisiopatológicas entre ambos trastornos, con respuesta similar del estreñimiento a fármacos comunes, siendo la diferencia fundamental la presencia o ausencia de dolor, pero no de un modo evaluable como «todo o nada». La gravedad de estos trastornos depende no solo de la intensidad de los síntomas intestinales sino también de otros factores biopsicosociales: asociación de síntomas gastrointestinales y extraintestinales, grado de afectación, y formas de percepción y comportamiento. Mediante los criterios de Roma, se diagnostican los trastornos funcionales intestinales. Esta Guía de práctica clínica está adaptada a los criterios de Roma IV difundidos a finales de mayo de 2016. En una primera parte (96, 97, 98 se analizaron los aspectos conceptuales y fisiopatológicos, los criterios de alarma, las pruebas diagnósticas y los criterios de derivación entre Atención Primaria y aparato digestivo

  20. Intestino de estrés

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    Vivian R. Mena Miranda

    Full Text Available El intestino se considera el mayor reservorio de gérmenes del organismo, aunque también desempeña un papel protagónico en la nutrición y regulación inmunológica. La isquemia intestinal es un factor predisponente de la disfunción múltiple de órganos (DMO, pues al redistribuirse el flujo sanguíneo para garantizar la perfusión de los órganos vitales se produce la liberación de mediadores de la inflamación y de endotoxinas, capaces de desencadenar un síndrome de respuesta inflamatoria sistémica con severas alteraciones hemodinámicas. La perfusión y nutrición enteral precoz se siguen considerando los 2 aspectos más importantes en la prevención del intestino de estrés.

  1. Infiltração de leucócitos e imunorreatividade antimieloperoxidase em granulócitos da mucosa e submucosa do intestino grosso de equinos submetidos à sobrecarga dietética com amido

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    Tiago Marques dos Santos

    2011-06-01

    Full Text Available This study was carried out to evaluate leukocyte infiltration and anti-myeloperoxidase immunoreactivity in granulocytes of the mucosa and submucosa of the large intestine of horses submitted to dietetic starch overload. Eight adult horses were allocated randomly in three treatments: Treatment I (Control (n = 2, animals euthanized without starch overload; and Treatments II (n = 3 and III (n = 3, animals undergoing starch overload, with gastric infusion of 17.6 g starch per kg of body weight, euthanized after 24 and 36 hours, respectively. Only afflux of neutrophils in the intestinal mucosa and submucosa blood vessels (leukocyte stasis was observed. Eosinophils were the predominant cells in the mucosa and submucosa in all horses, independent of dietetic overload, with infiltration grade from mild to moderate. Lymphocyte infiltration was also observed in all horses, but with lower intensity when compared to eosinophils. Congestion, edema and dilatation of lymphatic vessels were the main circulatory alterations observed, with more intensity in the submucosa. Higher immunoreactivity to the anti-myeloperoxidase antibodies was observed in the mucosa and submucosa of horses 36 hours after overload. Horses submitted to dietetic starch overload showed intestinal inflammatory response with prevalence of eosinophils, leukocyte stasis and circulatory alterations, varying from discreet to moderate.

  2. Desarrollo de una metodología in vitro para estimar la tasa de fermentación de los forrajes en el intestino grueso del cerdo

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    Leterme Pascal

    2005-12-01

    Full Text Available La metodología para estimar la tasa de fermentación de los forrajes se desarrolló en dos etapas. La primera, de digestibilidad enzimática, simula la digestión ocurrida en el estómago e intestino delgado, mediante el empleo de pepsina y pancreatina; en esta se determina la digestibilidad de la materia seca. La segunda etapa de fermentación simula la fermentación que ocurre en el intestino grueso utilizando como inóculo heces de cerdo; en esta se determina la cantidad de gas producido durante la fermentación de los forrajes y la degradación de la fibra detergente neutra (FDN. Las dos etapas presentaron buena repetibilidad en el tiempo. Abstract Develop of an in vitro methodology to determine the forages fermentation in to the large intestine. The methodology was developing in two phases. The first phase (enzymatic digestibility simulates the digestion occurred in the stomach and small intestine, by means of the employment of pepsin and pancreatin; and the digestibility of the dry matter is determined. The second phase (in vitro fermentation simulates the fermentation that occurs in the large intestine utilizing pig faeces; the quantity of gas produced during the fermentation of the feedstuff is determined and determine the degradation of neutral detergent fiber (NDF. All the phases show a good repeatability throw the time. Key words: digestibility in vitro, pigs, fermentación, forages (Azolla filiculoides, Xanthosoma saggitifolium, Morus alba, Trichanthera gigantea y Salvinia molesta.

  3. Efecto secuestrador del D-002 sobre radicales hidroxilo en mucosa gástrica

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    Yohani Pérez Guerra

    2012-03-01

    Full Text Available Introducción: el agente causal de la ulceración gástrica está asociado al desequilibrio entre factores agresivos y defensivos que actúan sobre la mucosa gástrica. El D-002, mezcla de seis alcoholes alifáticos primarios superiores purificada de la cera de abejas, produce efectos gastroprotectores mediados por múltiples mecanismos y reducción de la peroxidación lipídica en la mucosa gástrica. Objetivo: determinar si el D-002 es capaz de capturar el radical hidroxilo añadido in vitro o generado in vivo en ratas con úlcera gástrica inducida por indometacina. Métodos: En la experiencia in vitro el D-002 se añadió a concentraciones entre 0,9 y 1 000 mg/mL. En la experiencia in vivo las ratas se distribuyeron en seis grupos: un control negativo y cinco que recibieron indometacina: un control positivo tratado con el vehículo, tres con D-002 (5, 25, y 100 mg/kg, respectivamente, p.o. y otro con omeprazol (20 mg/kg i.p.. Los tratamientos se administraron una hora (vehículo y D-002 o 30 min (omeprazol, respectivamente, antes de inducir las úlceras. En ambas experiencias se tomaron alícuotas de mucosa gástrica, y se determinó el daño a la 2-desoxirribosa por el radical hidroxilo. Resultados: la administración oral del D-002, no in vitro, protegió a la 2-desoxirribosa del daño oxidativo de modo marcado, significativo y dependiente de la dosis con respecto al control positivo. Conclusiones: los resultados indican que la capacidad del D-002 (25 y 100 mg/kg administrado por vía oral para secuestrar el radical hidroxilo, generado en la mucosa gástrica por la indometacina, pudiera contribuir a sus efectos antioxidantes y gastroprotectores sobre el daño que los antiinflamatorios no esteroideos producen sobre la mucosa gástrica.

  4. El Síndrome de intestino corto como factor desencadenante de translocación bacteriana y del fallo multiorgánico

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    Zurita Romero, Manuel

    1993-01-01

    [spa] Está quedando claro que el tracto gastrointestinal no es un órgano pasivo sino que posee importantes funciones endocrinas, metabólicas, inmunológicas y de barrera junto a las de absorción de nutrientes. En situaciones normales la mucosa gastrointestinal íntegra ejerce una función de barrera para las bacterias y endotoxinas intraluminales, pero en determinadas circunstancias estas bacterias pueden alcanzar inicialmente los ganglios linfáticos mesentéricos e invadir los tejidos extraintes...

  5. Síndrome de intestino corto como factor desencadenante de translocación bacteriana y del fallo multiorgánico, El

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    Zurita Romero, Manuel

    1993-01-01

    Está quedando claro que el tracto gastrointestinal no es un órgano pasivo sino que posee importantes funciones endocrinas, metabólicas, inmunológicas y de barrera junto a las de absorción de nutrientes. En situaciones normales la mucosa gastrointestinal íntegra ejerce una función de barrera para las bacterias y endotoxinas intraluminales, pero en determinadas circunstancias estas bacterias pueden alcanzar inicialmente los ganglios linfáticos mesentéricos e invadir los tejidos extraintestinale...

  6. Anatomia Funcional e Morfometria do Intestino no Teleostei (Pisces de Água Doce Surubim (Pseudoplatystoma coruscans - Agassiz, 1829

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    Seixas Filho José Teixeira de

    2001-01-01

    Full Text Available Os objetivos do presente trabalho foram estudar a anatomia funcional e a morfometria comparativas nos intestinos médio e no reto, do peixe Teleostei, surubim, Pseudoplatystoma coruscans (Agassiz, 1829 (Siluriformes, Siluroidei, Pimelodidae, de hábito alimentar carnívoro, em duas classes de tamanho, visando fornecer referência à nutrição para o ajuste de diferentes sistemas de alimentação artificial para essa espécie nativa. Por meio destes estudos concluiu-se que o intestino do surubim, sob o ponto de vista morfológico, deve ser denominado, de intestino médio e reto, devido a presença da valva ileorretal e da invaginação valvar intestinal entre esses segmentos. Em relação ao padrão de enrolamento do intestino, apesar do plano geral do intestino médio e do reto ter sido mantido, as alças do intestino médio apresentaram arranjo indefinido, não tendo sido determinado um arranjo-padrão para a espécie. O arranjo intestinal é compatível ao da maioria de peixes carnívoros, ou predominantemente carnívoro, uma vez que seu intestino é quase retilíneo; contudo, as circunvoluções das alças finais do intestino médio talvez possam ser vistas como adaptações a um possível regime onívoro, preferencialmente carnívoro. As pregas intestinais encontram-se mais complexas e desenvolvidas no intestino dos exemplares da segunda classe de tamanho. Procurando estabelecer relações entre o arranjo das pregas das mucosas e a velocidade de transporte do alimento no intestino médio da espécie estudada, sugere-se que o padrão longitudinal, com numerosas anastomoses retardam o avanço do alimento em sentido aboral, o que possibilita maior período digestivo e, conseqüentemente, maior aproveitamento dos nutrientes, pela exposição do material alimentar à mucosa intestinal por período maior, além de contribuir para a preparação do bolo fecal. As pregas da mucosa próxima ao ânus têm direção longitudinal, sugerindo auxílio na

  7. Efecto secuestrador del D-002 sobre radicales hidroxilo en mucosa gástrica Scavenger effect of D-002 on hydroxyl radicals in the gastric mucosa

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    Yohani Pérez Guerra

    2012-03-01

    Full Text Available Introducción: el agente causal de la ulceración gástrica está asociado al desequilibrio entre factores agresivos y defensivos que actúan sobre la mucosa gástrica. El D-002, mezcla de seis alcoholes alifáticos primarios superiores purificada de la cera de abejas, produce efectos gastroprotectores mediados por múltiples mecanismos y reducción de la peroxidación lipídica en la mucosa gástrica. Objetivo: determinar si el D-002 es capaz de capturar el radical hidroxilo añadido in vitro o generado in vivo en ratas con úlcera gástrica inducida por indometacina. Métodos: En la experiencia in vitro el D-002 se añadió a concentraciones entre 0,9 y 1 000 mg/mL. En la experiencia in vivo las ratas se distribuyeron en seis grupos: un control negativo y cinco que recibieron indometacina: un control positivo tratado con el vehículo, tres con D-002 (5, 25, y 100 mg/kg, respectivamente, p.o. y otro con omeprazol (20 mg/kg i.p.. Los tratamientos se administraron una hora (vehículo y D-002 o 30 min (omeprazol, respectivamente, antes de inducir las úlceras. En ambas experiencias se tomaron alícuotas de mucosa gástrica, y se determinó el daño a la 2-desoxirribosa por el radical hidroxilo. Resultados: la administración oral del D-002, no in vitro, protegió a la 2-desoxirribosa del daño oxidativo de modo marcado, significativo y dependiente de la dosis con respecto al control positivo. Conclusiones: los resultados indican que la capacidad del D-002 (25 y 100 mg/kg administrado por vía oral para secuestrar el radical hidroxilo, generado en la mucosa gástrica por la indometacina, pudiera contribuir a sus efectos antioxidantes y gastroprotectores sobre el daño que los antiinflamatorios no esteroideos producen sobre la mucosa gástrica.Introduction: the etiology of the gastric ulceration is associated to the imbalance between aggressive and defensive factors acting upon the gastric mucosa. D-002, a mixture of 6 higher primary alcohols

  8. Síndrome de intestino irritable: Una perspectiva actualizada

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    David Felipe Gómez Álvarez

    2008-04-01

    Full Text Available El síndrome de intestino irritable (SII es un desordengastrointestinal funcional (DGIF que hace parte de lacategorización según los criterios Roma III, como undesorden funcional del intestino. Debido a su altaprevalencia aproximada del 4 al 20% a nivel mundial, seconsidera una enfermedad de gran impacto por afectar lacalidad de vida. La manifestación clínica depende delsíntoma predominante: dolor o disconfort, diarrea,estreñimiento o un cuadro mixto. Su diagnóstico esbásicamente clínico por medio de los criterios de Roma III,descartando enfermedad orgánica. Las alternativasterapéuticas del SII son para el control sintomático de laenfermedad y mejoría de la calidad de vida, nunca comotratamiento definitivo. El objetivo del artículo es producir unarevisión actualizada de la temática para conocer, entender yabarcar una patología que en los últimos años ha generadoun alto compromiso en el paciente.

  9. Biodistribuição do pertecnetato de sódio (Na99mTcO4) em ratos submetidos à ressecção extensa de intestino delgado

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    Chacon, Dâmaso de Araújo

    2007-01-01

    A ressecção extensa do intestino delgado resulta na síndrome do intestino curto com repercussão desabsortiva importante. Uma avaliação morfofuncional poderá ser necessária aos órgãos envolvidos no processo. O objetivo desse estudo foi avaliar a biodistribuição do pertecnetato de sódio em órgãos de ratos submetidos a ressecção extensa do intestino delgado, a capacidade adaptativa da mucosa intestinal remanescente e o comportamento da curva ponderal pós-operatória. Foram utilizados 21 ratos Wis...

  10. Transplante de intestino delgado Small intestine transplantation

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    Flávio Henrique Ferreira Galvão

    2003-06-01

    Full Text Available RACIONAL: Avanços da biotecnologia e o desenvolvimento de novas drogas imunossupressoras melhoraram os resultados do transplante de intestino delgado. Esse transplante é atualmente indicado para casos especiais da falência intestinal. OBJETIVO: A presente revisão realça os recentes desenvolvimentos na área do transplante de intestino delgado. MATERIAL E MÉTODO: Mais de 600 publicações de transplante de intestino delgado foram revisadas. O desenvolvimento da pesquisa, novas estratégias de imunossupressão, monitorização do enxerto e do receptor, e avanços na técnica cirúrgica são discutidos. RESULTADOS: Realizaram-se cerca de 700 transplante de intestino delgado em 55 centros: 44% intestino-fígado, 41% enxerto intestinal isolado e 15% transplante multivisceral. Rejeição e infecção são as principais limitações desse transplante. Sobrevida de 5 anos na experiência internacional é de 46% para o transplante de intestino isolado, 43% para o intestino-fígado e de cerca de 30% para o transplante multivisceral. Sobrevidas prolongadas são mais freqüentes nos centros com maior experiência. Em série de 165 transplantes intestinais na Universidade de Pittsburgh, PA, EUA, foi relatada sobrevida do paciente maior do que 75% no primeiro ano, 54% em 5 anos e 42% em 10 anos. Mais de 90% desses pacientes assumem dieta oral irrestrita. CONCLUSÃO: O transplante de intestino delgado evoluiu de estratégia experimental para uma alternativa viável no tratamento da falência intestinal permanente. Promover o refinamento da terapia imunossupressora, do manejo e prevenção de infecções, da técnica cirúrgica e da indicação e seleção adequada dos pacientes é crucial para melhorar a sobrevida desse transplante.BACKGROUND: Significant progress has been made in clinical small bowel transplantation over the last decade mainly due advances in biotechnology and new immunosuppressive regiments. This transplantation has now been indicated

  11. Guía de práctica clínica: síndrome del intestino irritable con estreñimiento y estreñimiento funcional en adultos: concepto, diagnóstico y continuidad asistencial. (Parte 1 de 2

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    F. Mearin

    2017-01-01

    Full Text Available En esta Guía de práctica clínica analizamos el manejo diagnóstico y terapéutico de pacientes adultos con estreñimiento y molestias abdominales, bajo el espectro del síndrome del intestino irritable y el estreñimiento funcional. Tienen una importante repercusión personal, sanitaria y social, afectando a la calidad de vida de los pacientes que las padecen. En el síndrome del intestino irritable con predomino del estreñimiento, este es la alteración deposicional predominante junto con dolor abdominal recurrente, hinchazón y distensión abdominal frecuente. El estreñimiento se caracteriza por la dificultad o la escasa frecuencia en las deposiciones, acompañado por esfuerzo excesivo durante la defecación o sensación de evacuación incompleta. La mayoría no tienen una causa orgánica subyacente, considerándose un trastorno funcional intestinal. Poseen muchas similitudes clínicas y fisiopatológicas, con respuesta similar del estreñimiento a fármacos comunes. La diferencia fundamental es la presencia o ausencia de dolor, pero no de un modo evaluable como «todo o nada». La gravedad depende tantro de la intensidad de los síntomas intestinales como de otros factores: asociación de síntomas gastrointestinales y extraintestinales, grado de afectación, formas de percepción y comportamiento. Los criterios de Roma diagnostican los trastornos funcionales intestinales. Esta Guía está adaptada a los criterios de Roma IV (mayo de 2016 y analiza, en esta primera parte, los criterios de alarma, las pruebas diagnósticas y los criterios de derivación entre Atención Primaria y Aparato Digestivo. En una segunda parte, se revisarán las alternativas terapéuticas disponibles (ejercicio, dieta, tratamientos farmacológicos, neuroestimulación de raíces sacras o cirugía, efectuando recomendaciones prácticas para cada una de ellas.

  12. Anatomia funcional e morfometria dos intestinos e dos cecos pilóricos do teleostei (pisces de água doce Brycon orbignyanus (Valenciennes, 1849

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    Seixas Filho José Teixeira de

    2000-01-01

    Full Text Available O objetivo do presente trabalho foi estudar a anatomia funcional e a morfometria comparativas nos intestinos médio e posterior e nos cecos pilóricos da piracanjuba (Brycon orbignyanus Valenciennes, 1849 (Characiformes, Characidae, Bryconinae. Foram conduzidos estudos anatomofuncionais e morfométricos nos intestinos médio e posterior e nos cecos pilóricos dessa espécie com hábito alimentar onívoro. Constatou-se, por meio destes estudos, que o padrão de enrolamento das alças intestinais em arranjo em N apresentou, na segunda classe de tamanho, freqüentes variações no intestino médio, mas foi compatível com os de outras espécies de hábitos alimentares similares. Os estudos da morfometria mostraram que o comprimento total do intestino e das alças intestinais e seus calibres, provavelmente, exercem função específica na absorção dos nutrientes. As relações entre o arranjo das pregas da mucosa e a velocidade de transporte do alimento no intestino médio sugerem que os padrões transversal e oblíquo retardam o avanço do alimento em sentido aboral, possibilitando maior período digestivo e melhor aproveitamento dos nutrientes, contribuindo para a preparação do bolo fecal. O comprimento e o calibre dos cecos pilóricos aumentam com o desenvolvimento do peixe, e o seu padrão de mucosa possui características anatômicas semelhantes às do intestino.

  13. Efecto antioxidante y citoprotector del Solanum tuberosum (papa en la mucosa gástrica de animales de experimentación

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    Miguel Sandoval-Vegas

    2010-07-01

    Full Text Available Introducción: Existen alternativas terapéuticas con productos naturales oriundos usados de manera empírica en la población. Tal es el caso del zumo de papa (Solanum tuberosum usado para problemas de mucosa gástrica. Objetivos: Evaluar la capacidad antioxidante y el efecto citoprotector a la mucosa gástrica del zumo de papa (Solanum tuberosum. Diseño: Experimental. Institución: Centro de Investigación de Bioquímica y Nutrición de la Facultad de Medicina, Universidad Nacional Mayor de San Marcos, Lima. Materiales biológicos: Solanum tuberosum, variedad Tomasa y ratas albinas machos. Métodos: Se administró vía oral post ayuno las fracciones de sobrenadante y sedimento del zumo de Solanum tuberosum. Una hora después se administró alcohol como injuria de mucosa gástrica. Por laparotomía abdominal se obtuvo el tejido gástrico. Se midió en la mucosa gástrica el estrés oxidativo por lipoperoxidación, la formación de moco por alcian blue y la protección midiendo la extensión del área lacerada en imagen digitalizada. Principales medidas de resultados: Capacidad antioxidante y efecto citoprotector a la mucosa gástrica. Resultados: El sobrenadante de la dosis 5 mL/ kg produjo mayor protección al estrés oxidativo; el precipitado en dosis 5 mL/kg presentó mayor producción de moco, sin superar al control. El precipitado 20 mL/kg produjo mayor citoprotección (73,8%. Conclusión: La fracción sobrenadante del zumo de la papa (Solanum tuberosum posee actividad de defensa antioxidante y la fracción del sedimento, mayor actividad citoprotectora de la mucosa gástrica.

  14. Intestino delgado y patologías asociadas a la malabsorción intestinal

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    Herrería Román, Esther

    2013-01-01

    La absorción intestinal es una función indispensable para el correcto desarrollo del ser humano. Sin embargo, no nos damos cuenta de lo vital que es hasta que sufrimos algún tipo de malabsorción que nos limita el desarrollo de una vida diaria plena. Expondremos en primer lugar la estructura, capas, células y función del intestino delgado, para comprender el desarrollo de algunas enfermedades que cursan con malabsorción, como la enfermedad de Crohn, la intolerancia a la lactosa y la intoleranc...

  15. Probióticos en síndrome de intestino irritable

    OpenAIRE

    Andrés G, Ortiz M

    2009-01-01

    En ésta revisión se analizan los posibles usos de los probióticos en el trata miento del síndrome de intestino irritable, evaluando la fisiología de la barrera intestinal, la interacción de la microbiota con dicha barrera y su repercusión en el sistema inmune y sobre el sistema nerviosos tanto autonómico como el sistema nerviosos central. Además, se analizan los distintos mecanismos fisiopatológico del mismo, y los mecanismos de acción de los probióticos, para posteriormente analizar su posib...

  16. Asociación del virus herpes humano 8 y la hiperplasia linfoide nodular difusa del intestino delgado en la inmunodeficiencia variable común Association between the human herpesvirus 8 and the diffuse nodular lymphoid hyperplasia of the small intestine in common variable immunodeficiency

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    Elena Kokuina

    2009-12-01

    Full Text Available La inmunodeficiencia variable común (IDVC es la inmunodeficiencia primaria más frecuente en el terreno clínico y sus formas de presentación son muy variables. Se describe una paciente con IDVC de adulto con síndrome diarreico crónico, pérdida de peso y linfoadenopatías difusas. Sus características inmunológicas más notables fueron una profunda hipogammaglobulinemia de las 3 clases mayores de inmunoglobulinas y la disminución numérica de las células B (CD19+ y células NK (CD3-CD56+ en sangre periférica. La biopsia del intestino delgado obtenida por panendoscopia asistida por video, reveló hiperplasia linfoide multinodular con atrofia parcial de las vellosidades. La inmunohistoquímica mostró que los nódulos consistían en centros germinales aumentados de tamaño con una distribución de células B (CD20+ y células T (CD3+, similar a la del folículo normal. No se encontró expresión diferencial de cadenas ligeras κ y λ. El método de la reacción en cadena de la polimerasa en tiempo real (QRT-PCR detectó un número apreciable de copias del genoma del virus del herpes humano tipo 8 (VHH-8 (133 copias/µL de ADN en el ADN del nódulo intestinal biopsiado. La infección con el VHH-8 puede ser un factor importante en la patogenia de los trastornos linfoproliferativos en pacientes con IDVC.The common variable immunodeficiency (CVID is the more frequent primary immunodeficiency in clinical field and its presentation forms are very variable. We describe the case of a women presenting with adult CVID with chronic diarrhea syndrome, weight loss and diffuse lymphadenopathies, where the more marked immunologic features were a deep hypogammaglobulinemia of the three major kinds of immunoglobulins and numerical decrease of B cells (CD19+ and NK cells (CD3-CD56+ in peripheral blood. Biopsy of small intestine obtained by video-assisted panendoscope, showed the presence of a multinodular lymphoid hyperplasia with partial atrophy of

  17. Efecto del manejo de la alimentación sobre la estructura de la mucosa ruminal del toro de lidia

    OpenAIRE

    Lomillos, Juan Manuel; Alonso, Marta Elena; González, José Ramiro; Gaudioso, Vicente Ramiro

    2017-01-01

    En el presente trabajo se ha evaluado el efecto producido sobre diversos parámetros fisiológicos indicativos de salud ruminal, de la utilización de una mezcla homogénea de alimento concentrado y forraje, como sistema de alimentación para la finalización, o cebamiento, del toro de lidia, comparándolo con un lote de animales testigo mantenidos en extensivo y otro de individuos alimentados siguiendo el sistema tradicional, basado en la administración de alimento concentrado y forraje por...

  18. Relaciones entre el tamaño del pelo, el color de la piel y mucosas externas con la producción lechera en condiciones tropicales

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    Manrique P. Luis Phanor

    1995-06-01

    Full Text Available

    El objetivo de la investigación fue determinar las relaciones entre el tamaño del pelo, el color de la piel y el de las mucosas externas (hocico y párpados con los rendimientos productivos de vacas lecheras Lucerna, explotadas en condiciones de clima tropical en Bugalagrande, Colombia. Se utilizaron 1855 registros con datos en 180 y 305 días de lactación, producción total de leche y la duración de los períodos de lactancia. A las vacas se les asignó una de las tres categorías para el tamaño del pelo, una de las cuatro para el color de la piel y una de las tres para el color de las mucosas externas. Por medio del método de cuadrados mínimos se realizaron los respectivos análisis de varianza y con el apoyo de pruebas de medias, se demostró que el tamaño del pelo no fue una fuente de variación significativa, pero las mayores producciones se presentaron en el grupo de vacas con pelo corto o fino. La relación entre el color de la piel y los rendimientos productivos determinó diferencias estadísticas (P < 0.05 en los grupos de animales, donde las mayores producciones se presentaron en los de las vacas con piel negra y café clara. El color de las mucosas externas no influyó significativamente en los rendimientos de las vacas lecheras.

    This research was conducted to find out the relationship among hair size, skin color and externals mucosa color (muzzle and evedil occular with milk production in cows of Lucerna's race, developed under tropical condition in Bugalagrande, Colombia. A total of 1855 records were analized in 180 and 305 days of milking, total production and lactation length. Each cow was classified under one of three categories for hair size, one of four categories for skin color and one of three categories for externals mucosa color. Harvey's statistical methods were used to analyse the data and the results showed up that the size of the hair was not a source of significative variation, but the larger milk

  19. Histologia, histoquímica e histometria do intestino de jacaré-do-Pantanal criado em cativeiro

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    Victor M. Aleixo

    2011-12-01

    Full Text Available A criação de jacaré-do-Pantanal tem se consolidado no estado de Mato Grosso como atividade alternativa e legal para as propriedades rurais na área de ocorrência natural da espécie, coibindo a caça predatória e colaborando na preservação da espécie. A racionalização do processo produtivo na criação de jacaré é uma ação relativamente nova, que permite obter pele de melhor qualidade e de utilização integral, diferentemente daquelas oriundas de animais da natureza. Considerando a importância dos intestinos como sede dos principais eventos relacionados à obtenção de nutrientes para o metabolismo corpóreo, este trabalho teve por objetivo caracterizar qualitativa e quantitativamente a mucosa dos intestinos delgado e grosso de jacaré-do-Pantanal jovens. Para a caracterização ao microscópio óptico da estrutura da parede intestinal e da histometria da mucosa foram coletadas amostras intestinais de 16 animais, sendo três deles também destinados ao estudo histoquímico. As amostras foram obtidas de cinco regiões, sendo quatro do intestino delgado e uma do intestino grosso. Elas foram fixadas em solução de Bouin, processadas de acordo com técnicas preconizadas para inclusão em parafina. Cortes histológicos semi-seriados (quatro micrômetros foram obtidos e o material foi desparafinizado, hidratado e corado pela hematoxilina-eosina para a descrição geral e estudo histométrico, que consistiu da mensuração da freqüência das vilosidades intestinais; dos comprimentos do epitélio e da lâmina própria da mucosa e da espessura da mucosa. Na análise estatística foi empregada a análise de variância e Teste de Tukey. Para o estudo histoquímico foram utilizadas a reação ao ácido periódico de Schiff, alcian blue pH 1,0 contrastado com hematoxilina e alcian blue pH 2,5 conjugado ao ácido periódico de Schiff. Para a descrição das estruturas histológicas foi empregada a terminologia disponível na Nomina

  20. Effect of probiotic species on irritable bowel syndrome symptoms: A bring up to date meta-analysis Efecto de los probióticos en los síntomas del síndrome del intestino irritable: un meta-análisis actualizado

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    María Ortiz-Lucas

    2013-01-01

    Full Text Available Background and objectives: immune system alteration in irritable bowel syndrome (IBS patients may be modulated by probiotics. We assessed the efficacy of some probiotic species in alleviating characteristic IBS symptoms. Material and methods: a meta-analysis of all identified randomized controlled trials comparing probiotics with placebo in treating IBS symptoms was performed with continuous data summarized using standardized mean differences (SMDs with 95% confidence intervals (95% CIs, where appropriate. The random-effects model was employed in cases of heterogeneity; otherwise, fixed-effects models were used. Results: meta-analysis was performed with 10 of 24 studies identified as suitable for inclusion. Probiotics improved pain scores if they contained Bifidobacterium breve (SMD, -0.34; 95% CI, -0.66; -0.02, Bifidobacterium longum (SMD, -0.48; 95% CI, -0.91; -0.06, or Lactobacillus acidophilus (SMD, -0.31; 95% CI, -0.61; -0.01 species. Distension scores were improved by probiotics containing B. breve (SMD, -0.45; 95% CI, -0.77; -0.13, Bifidobacterium infantis, Lactobacillus casei, or Lactobacillus plantarum (SMD, -0.53; 95% CI, -1.00; -0.06 species. All probiotic species tested improved flatulence: B. breve (SMD, -0.42; 95% CI, -0.75;-0.10, B. infantis, L. casei, L. plantarum (SMD, -0.60; 95% CI, -1.07; -0.13, B. longum, L. acidophilus, Lactobacillus bulgaricus, and Streptococcus salivarius ssp. thermophilus (SMD, -0.61; 95% CI, -1.01; -0.21. There was not a clear positive effect of probiotics concerning the quality of life. Conclusions: some probiotics are an effective therapeutic option for IBS patients, and the effects on each IBS symptom are likely species-specific. Future studies must focus on the role of probiotics in modulating intestinal microbiota and the immune system while considering individual patient symptom profiles.Antecedentes y objetivos: la alteración del sistema inmune en los pacientes con síndrome del intestino

  1. Treatment of irritable bowel syndrome with probiotics: An etiopathogenic approach at last? Tratamiento con probióticos del síndrome de intestino irritable: ¿Por fin un enfoque etiopatogénico?

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    M. Bixquert Jiménez

    2009-08-01

    Full Text Available Irritable bowel syndrome (IBS is the most common functional digestive disorder, and may affect 11-20% of the adult population in industrialized countries. In accordance with Rome III criteria (2006 IBS involves abdominal pain and bowel habit disturbance, which are not explained by structural or biochemical abnormalities. Several hypotheses attempt to account for the pathophysiology of IBS, but the etiology still remains uncertain or obscure, perhaps multifactorial. Abnormalities in colonic microflora have recently been suggested in such patients, as has abnormal small-intestine bacterial overgrowth (SIBO, or in particular a significant reduction in the amount of intraluminal Bifidobacteria or Lactobacilli, with consequences like the production of colonic gas, and motility or sensitivity disturbances of the intestinal tract. The disorder is difficult to treat, and the wide spectrum of non-drug and drug treatments shows our ignorance about the cause of the condition. Newer drugs, both pro- and anti-serotonin, have failed to show long-term efficacy or have been withdrawn due to concerns about harmful effects. Recent research has provided increasing support for the idea that disturbances of intestinal microbiota occur in patients with IBS, and that such abnormalities may contribute to IBS symptoms. Studies in Scandinavian countries in the last ten years emphasize the role of probiotics in the modulation of intestinal microbiota, and as a consequence in the regulation of the motility and hypersensitivity of the digestive tract. Although results between studies are difficult to compare because of differences in study design, probiotic dose, strain, and duration of therapy, some studies show symptom improvement. Lactobacilli are found among the normal bacterial flora of the gastrointestinal tract, and Lactobacillus plantarum (Lp is one of the species frequently isolated from the human mucosa, which is capable of surviving the low pH of the stomach and

  2. Evaluación de la irritabilidad en mucosa del adyuvante AFCO1 por el método de HET-CAM

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    Alexander Batista

    2011-04-01

    Full Text Available Los adyuvantes pueden producir irritación local en la mucosa y esto pudiera ser una limitación para su uso clínico. Para evaluar si el adyuvante vacunal AFCo1, un cocleato obtenido a partir del proteoliposoma de Neisseria meningitidis, produce irritación directa en la mucosa nasal, se estudió el efecto de su aplicación en la membrana corioalantoidea del embrión de pollo (MCA, por la técnica de HET-CAM (hen's egg test on chorioallantoic membrane, según el Protocolo 47 de INVITTOX, método alternativo que sustituye la clásica prueba de Draize en conejos. En este ensayo se utilizaron por cada producto a evaluar (AFCo1 o amortiguador fosfato salino como diluente tres huevos de gallinas White Leghorn de 10 días de embrionados; para los controles positivos de irritación (NaOH a 0,1 N y SDS al 1% se utilizaron dos huevos para cada uno. Los productos fueron aplicados en la MCA para evaluar las lesiones de lisis, hemorragia y coagulación, a los 5 min. Las sustancias se clasificaron según una escala establecida para productos no transparentes. Adicionalmente se realizó una evaluación microscópica de las MCA tratadas para confirmar las observaciones realizadas. Al determinar el grado de severidad de las tres reacciones, después de la aplicación de AFCo1 y del diluente, ambos clasifican como no irritantes, lo que evidencia que este cocleato no produce lesión epitelial directa. Este resultado, además, confirma la utilidad del HET-CAM para la determinación de irritabilidad nasal de adyuvantes vacunales.

  3. Alteraciones de la mucosa bucal causadas por la asociación entre el tabaco y los colutorios bucales con una concentración de alcohol del 26,9 %

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    Anna Christina Medeiros Fossati

    Full Text Available Se analizaron el epitelio y la capa de queratina de la mucosa oral de 15 ratas que durante 45 días fueron sometidas a una aplicación tópica de picadura de tabaco y de colutorio bucal con una concentración de alcohol del 26,9 %. Tras ese período, se extrajeron las mucosas y se les realizó un análisis histológico. Se observó una significativa disminución del espesor del epitelio y de la capa de queratina, lo que indica que la picadura asociada con colutorios bucales con alta concentración de alcohol, provoca la reducción del espesor de la capa de queratina y del epitelio de la boca.

  4. Abundancia y Biomasa de Lombrices de Tierra en dos Ecosistemas Intervenidos del Bosque Trópica l Húmedo, Bluefields Las lombrices de tierra son el intestino de la tierra Gilbert White, 1789.

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    Gladys Luna

    2012-09-01

    Full Text Available Muchos estudios han demostrado que las lombrices de tierra (Anelidae son el componente más abundante de la macrofauna del suelo y desempeñan funciones ecológicas dentro de él.El objetivo fue evaluar la abundancia y biomasa de lombrices de tierra en dos hábitats del bosque tropical húmedo con diferentes usos de suelo en la ciudad de Bluefields, Región Autónoma Atlántico Sur (RAAS. En cada hábitat se seleccionaron dos micro-hábitats: uno con sombra y otro sin sombra y en cada uno se delimitaron tres subparcelas de 1pie2 cada una, y una profundidad de 10 cm, ubicadas al azar y a una distancia mínima de 15 m entre sí.Cada muestra de suelo se tamizó y se extrajeron, se midieron y se pesaron los individuos por subparcela. El número de lombrices registradas fue mayor en el hábitat de área reforestada que en el hábitat de bosque, pero ocurrió lo contrario con respecto al tamaño promedio de los individuos.La mayor disponibilidad de materia orgánica en el bosque puede explicar el mayor tamaño de los individuos en este sitio.En aquellos suelos que han sufrido alteraciones antrópicas, como en el campus universitario, las comunidades de lombrices han experimentado cambios extremos denotando así una mala salud; sin embargo, estas comunidades se están recuperando, especialmente en los microhabitats con sombra.

  5. Contenido de fósforo fítico, actividad fitásica y absorción del fósforo de cereales en pollos

    OpenAIRE

    Hernández, Glenn; Godoy, Susmira; Chicco, Claudio F.

    2009-01-01

    Se determinó el contenido de fósforo total, fósforo fítico y actividad fitásica intrínseca del trigo duro, maíz amarillo y un sorgo alto en taninos y, en pollos de 4 semanas de edad, se midió la retención neta aparente del fósforo total y del fósforo fítico de los tres cereales, así como la actividad fitásica de la mucosa del intestino delgado de las aves. El contenido de fósforo total y del fósforo fítico fue similar a los valores generalmente reseñados para estos granos. La actividad fitási...

  6. Prevalencia y factores de riesgo de las lesiones de la mucosa oral en la población urbana del Uruguay

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    Beatriz Casnati

    Full Text Available Objetivo: El objetivo de este estudio fue determinar la prevalencia de las lesiones de la mucosa bucal e identificar los factores de riesgo en el desarrollo de las mismas en una muestra representativa de la población adulta urbana del Uruguay. Metodología: Se trata de un estudio transversal que registró 922 personas (537 F/385M, edades de 15-24, 35-44 y 65-74 años basado en un diseño muestral estratificado por conglomerado polietápico. A partir de los datos de la muestra se estimaron las prevalencias de cada entidad así como los intervalos de confianza al 95%. Resultados: La candidosis y las lesiones proliferativas se observaron en 26% y en 17% de las personas y se presentaron significativamente asociadas al género femenino. La prevalencia de la leucoplasia fue del 7% y en el análisis multivariado presentó una asociación significativa con el consumo de mate. Conclusiones: Las lesiones orales se presentan de manera prevalente en los adultos mayores en el Uruguay, lo que sugiere que se deberían implementar programas de prevención, diagnóstico y tratamiento adecuado de las mismas

  7. Lipo sarcoma in small intestine; Liposarcoma de intestino delgado

    Energy Technology Data Exchange (ETDEWEB)

    Rodriguez Iglesias, J; Pineyro Gutierrez, A; Taroco Medeiros, L; Fein Kolodny, C; Navarrete Pedocchi, H

    1987-12-01

    A case is presented by primitive liposarcoma in small intestine , an extensive bibliographical review foreigner and national in this case. It detach the exceptional of the intestinal topography of the liposarcomas; and making stress in the relative value of the computerized tomography and ultrasonography in the diagnose of the small intestine tumors . As well as in the sarcomas of another topography, chemo and radiotherapy associated to the exeresis surgery, it can be of benefit. [Spanish] Se presenta un caso de invaginacion yeyunal por liposarcoma primitivo de intestino delgado,realizandose una extensa revision bibliografica extranjera y nacional al respecto,destacando lo exepcional de la topografia intestinal de los piposarcomas; y haciendo hincapie en lo relativo valor de la tomografia computarizada y ultrasonografia en el diagnostico de los tumores de intestino delgado.Como en los sarcomas de otra topografia,la quimio y radioterapia asociadas a la cirugia de exeresis, puede ser de beneficio.

  8. Linfoma de celulas B de la zona marginal extraganglionar del tejido linfoide asociado a mucosa (linfoma MALT de pulmón

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    Carlos Vergara-Uzcategui

    2014-09-01

    Full Text Available Los Linfomas Pulmonares Primarios son extremadamente raros (0,4% de los linfomas extraganglionares, y generalmente son de tejido linfoide asociado a mucosas (tipo MALT, con ocasionales linfomas de células grandes difusos. Los síntomas son inespecíficos, y casi la mitad de los pacientes son asintomáticos. Se presenta el caso de un paciente masculino de 56 años de edad, quien presentó durante 7 meses accesos diarios de tos seca, de predominio nocturno, asociados en el último mes a fiebre de 39ºC, disnea a medianos esfuerzos y expectoración verduzca. Los exámenes de laboratorio fueron normales. La radiografía de tórax mostró el mediastino ensanchado, un proceso en lóbulo medio con efecto atelectásico, y un nódulo en hemitórax izquierdo. La tomografía computarizada (TC torácica de alta resolución evidenció proceso alveolar derecho en lóbulo medio y un nódulo pulmonar izquierdo calcificado de tipo inespecífico. El estudio inmunohistoquímico de la biopsia pulmonar fue compatible con Linfoma de Células B de la zona marginal extraganglionar del tejido linfoide asociado a mucosa (Linfoma MALT de pulmón. La TC corporal y la Tomografía por Emisión de Positrones (PET evidenciaron importantes hallazgos complementarios para determinar la extensión de la enfermedad. El paciente se trato con quimioterapia y actualmente se encuentra en buenas condiciones, sin recidiva de la sintomatología. Dado lo infrecuente de la patología se presenta este caso y se hace una revisión de la literatura

  9. Caracterização comparativa do intestino das espécies da Ordem Xenarthra

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    Marina M. Carvalho

    2014-12-01

    Full Text Available Resumo: O sucesso na manutenção de uma espécie depende de vários fatores entre eles a eficiência digestiva, sendo assim parâmetros morfométricos do tubo digestório são necessários para o conhecimento dos processos digestivos dos alimentos no organismo animal além de indicar a preferência alimentar de uma espécie. Este trabalho visou descrever morfologicamente os intestinos delgado e grosso, órgãos do sistema digestório de representantes da ordem Xenarthra a fim de fornecer subsídios para a avaliação da dieta e realização de procedimentos clínicos nestes animais, sejam eles de vida livre ou de cativeiro. Foram utilizados 7 espécimes entre preguiças-de-coleira (Bradypus torquatus, tatu-verdadeiro (Dasypus novemcinctus e tamanduá-bandeira (Myrmecophaga tridactyla. Todos as amostras foram processadas seguindo procedimentos de rotina efetuados nos laboratórios de Anatomia Animal e Histologia da FZEA/USP. Os intestinos de B. torquatus se apresentaram curtos e simples, enquanto que nos exemplares de D. novemcintus e M. tridactyla o intestino era longo e com algumas peculiaridades. No duodeno de todos os espécimes notamos a presença das glândulas de Brünner e estruturas para aumentar a superfície de absorção. Apenas em preguiças, o mesentério mantém o jejuno preso à parede dorsal da cavidade abdominal. O íleo representou a menor porção nas preguiças e tatus, exceto em tamanduáque apresentava o íleo como a maior parte depois do jejuno. O ceco em tatus e tamanduás apresentavam tamanho considerável e a presença de glândulas na mucosa, nestas espécies destacamos a funcionalidade do ceco, uma vez que este se apresentou repleto de restos alimentares. Na mucosa do cólon de todos os espécimes, haviam criptas de Lieberkühn, sendo mais numerosas em D. novemcinctus e M. tridactyla. Apenas em B. torquatus, o reto apresentou maior diâmetro e rigidez em relação ao cólon. No reto de todas as espécies estudadas, a

  10. Efecto antioxidante y citoprotector del tocosh de Solanum tuberosum ‘papa’ en la mucosa gástrica de animales de experimentación

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    Miguel Hernán Sandoval Vegas

    2015-01-01

    Full Text Available El tocosh es un producto alimenticio obtenido por una técnica de conservación andina y que tiene propiedades nutritivas y terapéuticas. Objetivo: Demostrar la capacidad antioxidante y el efecto citoprotector del tocosh de Solanum tuberosum ‘papa’ en la mucosa gástrica de animales de experimentación. Diseño: Experimental. Institución: Centro de Investigación de Bioquímica y Nutrición, Facultad de Medicina, Universidad Nacional Mayor de San Marcos, Lima, Perú. Material biológico: Tocosh seco y molido administrado a ratas albinas. Intervenciones: A 6 grupos de ratas albinas machos (200 ± 50 g se les dividió en: (GI solución NaCl 0,9%, 10 mL/kg;(GII etanol al 70% a 10 mL/kg; (GIII, IV y V Tocosh equivalente a 900 mg/kg, 1 800 mg/kg y 2 700 mg/kg, respectivamente, y (GVI sucralfato 30 mg/kg. En todos los casos, una hora después se indujo injuria con etanol 70° a 10 mL/kg y por laparotomía abdominal se obtuvo el tejido gástrico. Principales medidas de resultados: Porcentaje de citoprotección gástrica en imagen digitalizada por image analysis software for plant disease quantification y capacidad antioxidante por lipoperoxidación método espectrofotométrico de la reacción de especies reactivas al ácido tiobarbitúrico (TBARS. Resultados: La marcha fitoquímica identificó compuestos fenólicos, alcaloides, triterpenoides y esteroides, azúcares reductores y aminoácidos libres como metabolitos secundarios. Las dosis de 2 700 mg/kg y 900 mg/kg resultaron en 0,72 y 1,81 nmol/g tejido de lipoperoxidación, respectivamente. La dosis de 1 800 mg/kg protegió un 97% del área de la mucosa gástrica, 2 700 mg/kg un 95% y la de 900 mg/kg, 88% (p<0,05. La dosis de 1 800 mg/kg exhibió mejor efecto citoprotector y la de 2 700 mg/kg mejor actividad antioxidante, comparada con sucralfato 30 mg/kg. Conclusiones: El tocosh de Solanum tuberosum ‘papa’ tuvo efecto citoprotector y actividad antioxidante.

  11. HISTOLOGÍA Y MORFOMETRÍA DEL SISTEMA DIGESTIVO DEL SILÚRIDO BAGRE TIGRITO (Pimelodus pictus

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    CM Olaya

    2007-01-01

    Full Text Available El bagre tigrito Pimelodus pictus es un pez ornamental con gran aceptación en el mercado acuarista internacional, sin embargo, se desconocen muchos aspectos de la biología básica de esta especie. Por tal motivo, se realizó un estudio histológico y morfométrico del sistema digestivo a Àn de contribuir al diseño de futuras dietas. Se sacriÀcaron 7 individuos adultos de P. pictus de 10 ± 0,5 cm de longitud total con una sobredosis de MS-222 (0,5 g/l. Los especímenes se Àjaron en formaldehído al 4%, durante cinco días a 4 °C. Luego se siguió el procedimiento para técnica en paraÀna. Se realizaron cortes de 5 μm de espesor y se colo-rearon con H&E. El sistema digestivo presentó cuatro capas: mucosa, submucosa, muscular y serosa. En el esófago la mucosa estaba compuesta por epitelio plano estratiÀcado con gran número de células caliciformes, y una submucosa de tejido conectivo laxo, seguida por músculo estriado esquelético con dos orientaciones (circular y longitudinal. En todos los órganos evaluados la capa serosa fue muy delgada. En el estómago se identiÀcaron dos regiones, la pilórica y la cardíaca, encontrándose glándulas gástricas en esta última, ambas regiones presentaban una mucosa con epitelio cilíndrico simple, una submucosa de tejido conectivo laxo, y una capa de músculo liso con dos orientaciones (circular y longitudinal así mismo, este órgano fue el que exhibió el mayor espesor en la capa mucosa y muscular. La histología de la mucosa, la submucosa y la capa muscular del intestino fue similar a lo presentado por el estómago, aunque morfométricamente esta región fue la que exhibió los menores valores en todas las capas evaluadas. El estómago bien deÀnido y el intestino con pocos ciegos pilóricos hace suponer que P. pictus es de hábitos omnívoros con preferencia de los alimentos de origen animal.

  12. Efeitos de baixas pressões no balonete da máscara laríngea na mucosa faringolaríngea do cão Efectos de bajas presiones en el balón de la máscara laríngea en la mucosa faringolaríngea del can Effects of low laryngeal mask cuff pressure on the laryngopharyngeal mucosa of dogs

    Directory of Open Access Journals (Sweden)

    Regina Helena Garcia Martins

    2001-12-01

    Full Text Available JUSTIFICATIVA E OBJETIVOS: Lesões da mucosa faringolaríngea e compressões de vasos e de nervos têm sido relatadas e atribuídas às altas pressões no balonete da máscara laríngea (ML. O objetivo deste trabalho foi estudar em cães a mucosa faringolaríngea em contato com o balonete da ML sob baixas pressões e avaliar as condições ventilatórias durante a anestesia. MÉTODO: Em 8 cães sob anestesia com pentobarbital foi inserida ML de número 4, mantendo-se a pressão no balonete em 60 cmH2O. Os atributos: freqüência de pulso (FP, pressão arterial média (PAM, pressão inspiratória (PI, pressão expiratória final de CO2 (P ET CO2 e saturação de pulso de O2 (SpO2 foram estudados em 0 (controle, 30, 60, 90 e 120 minutos após a inserção da ML. Após eutanásia, realizou-se biópsias nas áreas da contato da mucosa faringolaríngea com a ML para exame à microscopia óptica (MO e eletrônica de varredura (MEV. RESULTADOS: Os atributos estudados mantiveram-se sem alterações significativas durante o experimento, ocorrendo apenas pequeno aumento dos valores da PAM e da P ET CO2 nos tempos finais do experimento. À MO, o epitélio da mucosa faringolaríngea apresentou-se sem alterações na grande maioria das áreas examinadas, mas em algumas áreas houve pequena infiltração inflamatória de polimorfonucleares neutrófilos e leve congestão na camada subepitelial, sem diferença significativa entre as áreas (p JUSTIFICATIVA Y OBJETIVOS: Lesiones de la mucosa faringolaríngea y compresiones de vasos y de nervios han sido relatadas y atribuidas a las altas presiones del balón de la máscara laríngea (ML. El objetivo de este trabajo fue estudiar en canes la mucosa faringolaríngea en contacto con el balón de la ML bajo bajas presiones y evaluar las condiciones ventilatorias durante la anestesia. MÉTODO: En 8 canes bajo anestesia con pentobarbital fue introducida ML de número 4, manteniéndose la presión en el balón en 60 cm

  13. Histologia do intestino do avestruz (Struthio camelus, Linnaeus 1758

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    Gisele Saviani

    2013-08-01

    Full Text Available A despeito de o avestruz (Struthio camelus compartilhar muitas adaptações evolucionárias presentes em outras aves, estes animais apresentam algumas características anatômicas peculiares, como é o caso do seu tubo digestivo em que o cólon é maior que o ceco. Há algum tempo, essa ave tem sido explorada econômicamente e principalmente como fonte alternativa de proteína animal na alimentação humana. O presente trabalho analisou os aspectos histológicos do intestino de avestruzes produzidos em boas condições de manejo ambiental e nutricional. Foram utilizados 13 avestruzes, com 18 a 30 meses de idade, provenientes da empresa Brasil Ostrich, e encaminhados para o abate no Abatedouro Escola da Universidade de São Paulo, Campus Administrativo de Pirassununga. Os animais foram abatidos com pistola pneumática e, após a sangria e evisceração, foram colhidas amostras de diferentes segmentos do intestino: duodeno, jejuno, íleo e ceco duplo. Os materiais foram processados, corados pela técnica de hematoxilina-eosina (H-E e examinados em microscopia de campo claro. Os resultados obtidos revelaram que as vilosidades estão presentes no duodeno, porém, não existem no ceco. Dos quatro segmentos intestinais examinados, o ceco foi o que apresentou maior número de células caliciformes. Os nódulos linfáticos e os linfócitos foram observados em todos os segmentos examinados. No ceco, os nódulos linfáticos se agregam para constituir a placa de Peyer. O plano histológico dos segmentos intestinais examinados seguiu o padrão observado nos mamíferos domésticos e em outras aves. O conhecimento da histologia dos intestinos desses animais pode oferecer subsídios para a avaliação comparativa de procedimentos de manejo ambiental e nutricional que possam aumentar os níveis de produção e produtividade dessa atividade pecuária.

  14. Efeitos da pressão limite (25 cmH2O e mínima de “selo” do balonete de tubos traqueais sobre a mucosa traqueal do cão Efectos de la presión limite (25 cmH2O y mínima de “sello” del balón de tubos traquéales sobre la mucosa traqueal del can Effects of tracheal tube cuff limit pressure (25 cmH2O and “seal” pressure on tracheal mucosa of dogs

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    Emanuel Celice Castilho

    2003-12-01

    Full Text Available JUSTIFICATIVA E OBJETIVOS: As lesões da mucosa traqueal em contato com o balonete do tubo traqueal são proporcionais à pressão exercida pelo balonete e ao tempo de exposição. O objetivo foi estudar as eventuais lesões da mucosa do segmento traqueal em contato com o balonete do tubo traqueal insuflado com volume de ar suficiente para se obter pressão de “selo” ou com a pressão limite de 25 cmH2O, abaixo da pressão crítica de 30 cm de água para produção de lesão da mucosa traqueal. MÉTODO: Dezesseis cães foram submetidos à anestesia venosa e ventilação artificial. Os cães foram distribuídos aleatoriamente em dois grupos de acordo com a pressão no balonete do tubo traqueal (Portex Blue-Line, Inglaterra: Gselo (n = 8 balonete com pressão mínima de “selo” para impedir vazamento de ar durante a respiração artificial; G25 (n = 8 balonete insuflado até obtenção da pressão de 25 cmH2O. A medida da pressão do balonete foi realizada por meio de manômetro digital no início (controle e após 60, 120 e 180 minutos. Após o sacrifício dos cães, foram feitas biópsias nas áreas da mucosa traqueal adjacentes ao balonete e ao tubo traqueal para análise à microscopia eletrônica de varredura (MEV. RESULTADOS: A pressão média do balonete em G25 manteve-se entre 24,8 e 25 cmH2O e em Gselo entre 11,9 e 12,5 cmH2O durante o experimento. As alterações à MEV foram pequenas e não significantemente diferentes nos grupos (p > 0,30, mas ocorreram lesões mais intensas nas áreas de contato da mucosa traqueal com o balonete do tubo traqueal, nos dois grupos, em relação às áreas da mucosa adjacentes ou não ao tubo traqueal (p JUSTIFICATIVA Y OBJETIVOS: Las lesiones de la mucosa traqueal en contacto con el balón del tubo traqueal son proporcionales a la presión ejercida por el balón y al tiempo de exposición. La finalidad fue estudiar las eventuales lesiones de la mucosa del segmento traqueal en contacto con el bal

  15. Involved mechanisms in the radioprotector effect of the insulinic-1 type growth factor (IGF-1) in the mucous of the small intestine; Mecanismos involucrados en el efecto radioprotector del factor de crecimiento tipo insulinico-1 (IGF-1) en la mucosa del intestino delgado

    Energy Technology Data Exchange (ETDEWEB)

    Mohamad, N.; Medina, V.; Sambuco, L.; Gutierrez, A.; Nunez, M.; Martin, G.; Cricco, G.; Rivera, E.; Bergoc, R. [Laboratorio de Radioisotopos, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires. Junin 956, Buenos Aires (Argentina); Croci, M.; Crescenti, E. [Instituto de Inmunooncologia, Cordoba 3200, Buenos Aires (Argentina)]. e-mail: rmbergoc@ffyb.uba.ar

    2006-07-01

    The use of radiant therapies in malignant tissues presents the inconvenience of affecting also to the healthy tissues, mainly when these present a high rate of proliferation like in the case of the mucous of the small intestine. The growth factor of insulinic-1 type (IGF-1) it has been pointed out as a possible protector of normal tissues under irradiation conditions. The objective of this work was to evaluate the effect of the IGF-1 like radioprotector of the mucous of the small intestine in mice irradiated with 10 Gy to whole body, determining the histological characteristics of the tissue, the presence of apoptotic cells, the expression of antigen of cellular proliferation (PCNA) and of anti-oxidant enzymes. Four groups of mice were used: control, treated with IGF-1, irradiated and irradiated and treated with IGF-1. The two treated groups were injected subcutaneously with two dose by day of 2.5 {mu}g of IGF-I /0.1ml during four days (days 1 at 4). The two irradiated groups 10 Gy received to whole body the day 2. The day 5 all the animals were sacrificed and cuts of the mucous of the small intestine were obtained. The histological cuts were evaluated by tint with hematoxyline-eosin; the presence of apoptotic cells its were determined by the Tunnel method (Apoptag kit); the expression of PCNA, superoxide dependent dismutase of copper and zinc (CuZnSOD), superoxide dependent dismutase of manganese (MnSOD), catalase (CAT) and glutathion peroxidase (GPX), by immunohistochemistry. The results demonstrated that the treatment with IGF-1 preserves the partially histology of the mucous of the intestine, the expression of PCNA and the presence of apoptotic cells in the crypts in front of the irradiation. The CuZnSOD it was expressed mainly in the hairiness and, in smaller measure, in the crypts increase in the group IR+IGF-1. The IGF-1 produced the expression of MnSOD in the crypts and in the intestinal hairiness. The expression of CAT in the hairiness increase significantly in the group treated with IGF-1 and irradiated, with regard to the other groups. We conclude that the treatment with IGF-1 induces the repair and/or proliferation in the mucous intestinal irradiated, it prevents the apoptotic cellular death and it modulates the expression of anti-oxidant enzymes positively, acting by this way as radioprotector of the same one. (Author)

  16. Probióticos, prebióticos y simbióticos en el síndrome de intestino irritable

    OpenAIRE

    Guzmán Calderón, Edson; Montes Teves, Pedro; Monge Salgado, Eduardo

    2014-01-01

    El síndrome del intestino irritable es un trastorno común que afecta a millones de personas en todo el mundo. Dicho transtorno tiene un impacto significativo en la calidad de vida de las personas que lo padecen afectando en mayor o menor medida la esfera sociolaboral de estos individuos con el consecuente sufrimiento individual e impacto macroeconómico por aumento en las tasas de ausentismo y rendimiento laboral. Los prebióticos y probióticos son suplementos de la dieta no digerible, elemento...

  17. Leishmaniasis mucosa y otras lesiones destructivas centrofaciales

    Directory of Open Access Journals (Sweden)

    G. Rodríguez

    1994-12-01

    Full Text Available Varias enfermedades producen lesiones del área centrofacial. Una de las más frecuentes en nuestro medio es la leishmaniasis destructivas mucosa. Como el INS es un centro de estudio de leishmaniasis, se atendieron pacientes o sus biopsias con afecciones del macizo centrofacial, primariamente remitidas con el diagnóstico clínico de leihsmaniasis mucosa. En un período de 7 años (1987-1993, se estudiaron 134 biopsias de estos pacientes. El diagnóstico de leishmaniasis mucosa fue definitivo en 26 casos, por demostración del amastigote con la coloración de hematoxilina eosina y el mismo diagnóstico se estableció por patrón histopatológico solamente, en 27 casos. Una técnica inmunoenzimática para demostrar los amastigotes no fue satisfactoria. La perforación banal del tabique nasal (52 biopsias es la entidad que el clínico y el patólogo confunden con mayor frecuencia con la leishmaniasis mucosa. Otras entidades demostradas fueron la paracoccidioidomicosis (3, histoplasmosis (2, rinosporidiosis (2. esporotricosis (l, tuberculosis bucal (3, lepra leprornatosa (l, escleroma nasal (2, granulomatosis de Wegener (2, linfomas angiocéntricos (4, aspiración crónica de cocaína (l, y carcinoma escamocelular palatino (1. La biopsia, luego de la historia clínica, es el pilar esencial para identificar y manejar adecuadamente estas lesiones, que pueden originar destrucción facial grave, a veces letal, si no se dispone del diagnóstico preciso y del tratamiento oportuno.

  18. Características histomorfométricas do intestino de juvenis de tambaqui após uso de probiótico na dieta e durante transporte

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    Celma M. Ferreira

    2014-12-01

    Full Text Available Avaliou-se neste trabalho as características histomorfométricas da mucosa intestinal de tambaqui após uso de probiótico a base de Bacillus spp., veiculado na ração e dissolvido na água durante transporte. Foi utilizado um delineamento inteiramente casualizado com três tratamentos e sete repetições, sendo comparado um tratamento controle (peixes alimentados com ração comercial sem probiótico a dois outros tratamentos envolvendo o uso de probiótico comercial, veiculado na ração ou na água de transporte. Um total de 510 juvenis de tambaqui (peso e comprimento total médio inicial de 83,26±28,14g e 17,39±1,90cm, respectivamente foi distribuído em três tanques de alvenaria. Durante 60 dias, os peixes de dois tanques foram alimentados com ração comercial e os do terceiro tanque com ração comercial suplementada com probiótico. Após este período, os peixes de cada tanque foram divididos em sacos plásticos e transportados por 4 horas de acordo com os seguintes tratamentos: T1 = alimentação com ração comercial (controle; T2 = alimentação com ração comercial e probiótico adicionado na água durante o transporte (20mg/L; T3 = alimentação com ração comercial suplementada com probiótico (1,0g/kg de ração. Antes do transporte (basal, 24 e 96 horas após o transporte, oito peixes de cada tratamento foram submetidos a eutanásia e o intestino retirado para pesagem e mensuração do comprimento para estabelecimento da relação comprimento corporal/ intestino. Em seguida, foi realizada coleta da porção anterior e posterior do intestino, para avaliação das características morfo-histológicas da mucosa intestinal. O uso de probiótico durante o transporte não afetou o peso do intestino de juvenis de tambaqui. O comprimento do intestino do tambaqui não foi alterado pelo tratamento, sendo observada somente relação linear entre o comprimento intestinal e o comprimento corporal (CC dos peixes. A suplementação com

  19. Síndrome de intestino corto en niños: actualidades en su diagnóstico y manejo

    Directory of Open Access Journals (Sweden)

    D. Valdovinos

    2012-07-01

    Se realizó una búsqueda de artículos en PubMed, considerando la información existente en niños con SIC de 20 años hasta la fecha, utilizando las palabras clave “síndrome de intestino corto”. De un total de 784 artículos potenciales, se realizó una selección de 82 artículos donde realizamos la revisión de la literatura médica. Los pacientes con SIC son todo un reto para su tratamiento, por lo que se debe establecer un manejo multidisciplinario enfocado en mantener un soporte nutricional óptimo que cubra las necesidades para crecimiento, desarrollo y asimismo, disminuir al máximo la presencia de complicaciones a corto, mediano y largo plazo. El diagnóstico y manejo de un niño con SIC, implica un equipo de profesionales expertos en el manejo gastroenterológico, pediátrico y nutricional. El pronóstico del niño estará en función al manejo oportuno, así como longitud de la resección intestinal y presencia o no de válvula ileocecal.

  20. HISTOLOGÍA DEL SISTEMA DIGESTIVO DE Didelphis albiventris (LUND, 1840

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    C. Moreno-Durán1

    2007-06-01

    Full Text Available Didelphis albiventris es un marsupial que se encuentra en Colombia en la región Andina desde los 1800 hasta 4000 m.s.n.m (Cuartas - Calle 2003. Su importancia ecológica radica en que es una especie quecontrola poblaciones de roedores, e insectos y contribuye a la dispersión de las semillas.Se llevó a cabo un estudio histológico del sistema digestivo de marsupiales de Didelphis albiventris, provenientes del municipio de Sotaquirá (Boyacá, Colombia. Los órganos se fijaron en formol buferizado al 10%, y se tiñeron con Hematoxilina - Eosina de acuerdo a la técnica de Propath 1992.Se realizaron descripciones histológicas, desde la cavidad oral hasta el intestino grueso. En la lengua se observaron papilas filiformes y tres papilas circunvaladas dispuestas en forma de triángulo en la parte posterior de la lengua, con botones gustativos. El estómago del marsupial se dividió en varias zonas: cardial, fúndica, del cuerpo y pilórica las cuales presentan características similares a la mayoría de mamíferos. En la primera porción del intestino delgado, se observó el duodeno con ausencia de glándulas de Brunner, la disposición de las fibras musculares lisas presentó variación a nivel de duodeno y cloaca, caracterizándose por poseer una muscular circular externa y una muscular longitudinal interna. No se observaron diferenciassignificativas con respecto a otros mamíferos, como roedores y el hombre en hígado, páncreas, vesícula biliar y glándulas salivares por lo cual no se incluyen en el presente trabajo. Se encontraron diferencias con respecto a los mamíferos en la ubicación de las glándulas de Brunner en el íleon y no en el duodeno y la distribución de nódulos linfoides dentro de la lámina propia de la mucosa de todo el tracto gastrointestinal.

  1. Alteraciones de la mucosa bucal causadas por la asociación entre el tabaco y los colutorios bucales con una concentración de alcohol del 26,9 % Alterations of the oral mucous membrane caused by the association between tobacco and the mouthwashes with an alcohol concentration of 26.9 %

    Directory of Open Access Journals (Sweden)

    Anna Christina Medeiros Fossati

    2006-09-01

    Full Text Available Se analizaron el epitelio y la capa de queratina de la mucosa oral de 15 ratas que durante 45 días fueron sometidas a una aplicación tópica de picadura de tabaco y de colutorio bucal con una concentración de alcohol del 26,9 %. Tras ese período, se extrajeron las mucosas y se les realizó un análisis histológico. Se observó una significativa disminución del espesor del epitelio y de la capa de queratina, lo que indica que la picadura asociada con colutorios bucales con alta concentración de alcohol, provoca la reducción del espesor de la capa de queratina y del epitelio de la boca.The epithelium and the keratin layer of the oral mucous membrane of 15 rats that were subjected to a topical application of cut tobacco and mouthwashes with an alcohol concentration of 26.9 % during 45 days were analyzed. After that period, the oral mucous membranes were removed and a histological analysis was made. It was observed a significant decrease of the thickness of the epithelium and of the keratin layer, which shows that cut tobbaco associated with collutories with a high concentration of alcohol causes the reduction of the thickness of the keratin layer and of the mouth epithelium.

  2. DESCRIPCIÓN MORFOLÓGICA DEL TUBO DIGESTIVO DE JUVENILES DE RUBIO Salminus affinis (PISCES: CHARACIDAE

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    VJ Atencio

    2008-09-01

    Full Text Available El objetivo del estudio fue la descripción del tubo digestivo de juveniles de Rubio Salminus affinis. Se analizaron cinco juveniles con 23,6±2,6 cm de longitud total y 142,8±62,5 g de peso. Se realizó descripción topográfica y morfológica de los órganos y cortes histológicos con tinción H-E. El esófago es un órgano tubular corto de pared gruesa que representa el 7,8% de la longitud total del tubo digestivo, presenta pliegues internos que ofrecen gran capacidad de distensión permitiendo el paso de presas de gran tamaño. El estómago es una bolsa asimétrica en forma de “Y”, grande y musculosa, amplio en la porción anterior y estrecho hacia el fondo; el número de ciegos pilóricos varió entre 13 y 23 pudiendo ser monotubulares y ramificados. El intestino, con tres asas, se extiende desde el esfínter pilórico hasta el ano. Histológicamente el tubo digestivo está formado por cuatro capas: mucosa, submucosa, muscular y serosa; el epitelio de revestimiento del esófago es estratificado plano no queratinizado con células caliciformes, cambiando a simple cilíndrico mucosecretor llegando al estómago; en el estómago se encuentra epitelio simple cilíndrico mucosecretor y los ciegos pilóricos e intestino son revestidos por epitelio simple cilíndrico con células caliciformes. Tanto el valor promedio del coeficiente intestinal (0,58, como la pared distensible del estómago sugieren que el Rubio tiene un tubo digestivo propio de carnívoro y sus características histológicas presentan similitud con la mayoría de vertebrados superiores.

  3. Acute lower gastrointestinal hemorrhage originating in the small intestine Hemorragia digestiva baja severa originada en el intestino delgado

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    A. Ríos

    2006-03-01

    las etiologías más frecuentes se localizan a nivel colónico. El objetivo es analizar el manejo diagnóstico-terapéutico de las HDB severas cuya etiología se localizaba en el intestino delgado. Pacientes y métodos: entre 1975 y marzo del 2002 ingresaron en nuestro servicio 12 HDB severas originadas en el intestino delgado. Todos consultaron por rectorragia franca, precisando al menos transfusión de 3 unidades de concentrados de hematíes. La edad media fue de 54 ± 21 años, el 58% eran mujeres, y el 83% había presentado cuadros previos de HDB. Resultados: en once casos (92% se realizó una endoscopia digestiva alta y baja urgente no objetivando el origen del sangrado. La arteriografía se indicó en 7 pacientes (58%, localizando en 5 de ellos el sangrado. En dos casos se hizo una gammagrafía mostrando en uno un divertículo de Meckel y en el otro fue normal. Todos se intervinieron, 8 (67% de urgencias, encontrándose un tumor en 9 casos y un divertículo de Meckel en 3, realizándose resección de todas las lesiones. La histología informó de leiomioma en 7 casos, de divertículo de Meckel en 3, de leiomioblastoma en 1 y de angioma en el caso restante. Tras un seguimiento medio de 132 ± 75 meses, fue éxitus el leiomioblastoma, y recidivó el angioma, el cual fue embolizado radiológicamente con éxito. Conclusiones: la HDB severa de origen en intestino delgado debe considerarse como posibilidad etiológica cuando la endoscopia digestiva no objetiva el origen del sangrado, siendo la arteriografía una técnica diagnóstica útil para localizar el sangrado. La cirugía supone el tratamiento definitivo del proceso, confirma la etiología, y descarta la presencia de malignidad.

  4. Antibiotic concentrations in intestinal mucosa.

    Science.gov (United States)

    Malmborg, A S

    1985-01-01

    The concentrations in the intestinal mucosa after the initial dose of cefoxitin, piperacillin and clindamycin have been studied. The antibiotics were given at the induction of anesthesia as prophylaxis to patients undergoing elective colorectal surgery. The concentrations of the antibiotics in serum and intestinal mucosa taken during the operation were determined by the microbiological agar diffusion method. Therapeutic concentrations in intestinal mucosa were maintained during the major part of the operation period. The mean mucosa/serum concentration ratios were for cefoxitin 0.4, for piperacillin 0.5 and for clindamycin 1.2.

  5. Actualización sobre síndrome de intestino corto y transplante intestinal

    OpenAIRE

    Duro, Debora; Kamin, Daniel

    2007-01-01

    El síndrome de intestino corto es una entidad médico-quirúrgico, con potencial riesgo para poner en peligro la vida de los niños, y que en su manejo incluye nutrición artificial. El estudio diagnóstico y terapéutico se logra idealmente con un equipo multidisciplinario compuesto de nutricionista, químico, trabajadora social, médico y cirujano. Uno de los primeros pasos, es la identificación anticipada de pacientes a riesgo de presentar complicaciones severas por el uso prolongado de nutrición ...

  6. Evaluación de Lactobacillus plantarum en intestino grueso de lechones por microscopía electrónica y química sanguínea

    Directory of Open Access Journals (Sweden)

    Henry Jurado G.

    2011-05-01

    Full Text Available Objetivo. Evaluar la presencia de Lactobacillus plantarum en intestino grueso de lechones. Materiales y métodos. 50 lechones fueron asignados al azar a 5 tratamientos (n=10. (T0: sin probiótico; T1: con L. plantarum 1 H1; T2: con L. plantarum 1 H2; T3: con probiótico comercial; T4: sin probiótico comercial. Después de los tratamientos, 3 lechones de cada grupo fueron seleccionados aleatoriamente para obtener muestras de intestino grueso para análisis por microscopía electrónica y suero para química sanguínea. La inmunoglobulina A (IgA se hizo por turbidimetría; colesterol total y nitrógeno ureico (BUN por espectofotometría. El recuento diferencial de leucocitos y polimorfonucleares (PMN neutrófilos mediante extendido de sangre. Resultados. Se comprobó la adhesión de L. plantarum 1 H1 y y L. plantarum 1 H2 en el intestino grueso. Se observó secreción de mucina y en la lámina propia inflamación y edema del tejido conectivo. La IgA mostró concentraciones altas en T2 (L. plantarum 1 H1 con 333 mg/100 mL y T3 (L. plantarum 1 H2 con 300 mg/100 mL. Los valores en T3 y T2 en los polimorfonucleares neutrófilos fueron elevados (65% y 55% respectivamente. El colesterol total fue menor en T2 y T3 con valores de 113.83 y 93.8 mg% respectivamente. El BUN para T2 y T3 fue el más bajo con 7.83 y 8.76 mg% respectivamente. Conclusiones. La utilización de probióticos con L. plantarum 1 adicionado en la ración mostró un efecto positivo en la colonización y adhesión en el intestino grueso, así como, una respuesta positiva en su sistema inmune.

  7. Estudio de nuevos marcadores de inflamación en enfermedades del intestino

    OpenAIRE

    Olmos Juste, Raquel

    2017-01-01

    La enfermedad celiaca (EC) se define como un proceso autoinmune multisistémico debido a una pérdida de tolerancia frente a proteínas de la dieta como al gluten de cereales. Las proteínas que causan esta enfermedad reciben el nombre de prolaminas y están presentes en el trigo (gliadinas), cebada (hordeínas), centeno (secalinas) y avena (aveínas). El único tratamiento que actualmente existe se basa en realizar una dieta libre de gluten (DSG), que da lugar a la desaparición de los...

  8. Utilização de probiótico em rações com diferentes níveis de proteína sobre o comprimento e a morfometria do intestino delgado de codornas de corte = Use of probiotic on diets with different protein levels on the length and morphometry of the small intestine of meat quails

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    Luciana Kazue Otutumi

    2008-07-01

    Full Text Available O trabalho objetivou avaliar o efeito do probiótico associado a diferentes níveis de proteína bruta (PB sobre o comprimento e a morfometria da mucosa do intestino delgado de codornas de corte. Foram utilizadas 2.304 codornas de corte, distribuídas em umdelineamento experimental inteiramente casualizado em esquema fatorial 2 x 4 (com e sem probiótico; quatro níveis de PB – 15, 20, 25 e 30%, com duas repetições por tratamento, em dois períodos experimentais. Aos sete, 14, 21 e 35 dias de idade, foram abatidas duas aves de cada repetição para avaliação do comprimento do intestino delgado (CID e a morfometria da mucosa do duodeno e íleo. O probiótico não influenciou o CID e a morfometria da mucosa do intestino delgado. O comprimento do intestino aumentou de maneira linear com a elevação dos níveis de PB aos sete, 14 e 21 dias, e a morfometria da mucosa aumentou de forma linear somente para altura vilo do íleo. Pode-se concluir que, nas condições ambientais em que as codornas foram criadas, apenas o nível de proteína influenciou o comprimento do intestino delgado e a altura de vilo do íleo, não sendoobservado efeito do probiótico sobre estes parâmetros.The aim of this study was to evaluate the effect of probiotic associated to different levels of crude protein (CP on thelength and mucous morphometry of the small intestine of meat quails. The study used 2,304 meat quails, distributed in a completely randomized experimental design in a 2 x 4 factorial scheme (with and without probiotic; four levels of CP – 15, 20, 25 and 30%, withtwo replications per treatment, in two experimental periods. At seven, 14, 21 and 35 days of age, two quails of each replication were slaughtered in order to evaluate the length of the small intestine (LSI, as well as duodenum and ileum mucous morphometry. LSI and smallintestine mucous morphometry were not influenced by probiotic. Intestine length increased in a linear fashion with the increase

  9. Infecciones virales en piel y mucosas

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    María José Martínez G., Dra.

    2011-11-01

    Full Text Available Diversos virus pueden infectar la piel y las mucosas de los seres humanos, manifestándose en diferentes patologías, de acuerdo a la edad, género y estado inmunológico. Un grupo importante de éstos tiene capacidad de replicar en células epiteliales, originando cuadros clínicos en los cuales se evidencia la destrucción celular o bien la hiperplasia del tejido infectado. Gran parte de estos virus persisten en células infectadas por años y tienen la capacidad de reactivarse y de manifestar nuevamente enfermedades cutáneas o mucosas. En algunos casos, ésta sólo se expresa de manera subclínica en una excreción del virus por secreciones que son fuente de infección. En los últimos años, los avances en técnicas moleculares han permitido contar con metodologías diagnósticas cada vez más sensibles, específicas y rápidas. Así como también el progreso en el área de vacunas y antivirales nos entrega mayores herramientas profilácticas y terapéuticas.

  10. Biodistribution of the radiophamarceutical sodium pertechnetate (Na99mTcO4 after massive small bowel resection in rats Biodistribuição do radiofármaco pertecnetato de sódio (Na99mTcO4 em ratos submetidos a ressecção extensa de intestino delgado

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    Dâmaso de Araújo Chacon

    2007-12-01

    Full Text Available PURPOSE: To evaluate the biodistribution of sodium pertecnetate (Na99mTcO4 in organs and tissues, the morphometry of remnant intestinal mucosa and ponderal evolution in rats subjected to massive resection of the small intestine. METHODS: Twenty-one Wistar rats were randomly divided into three groups of 7 animals each. The short bowel (SB group was subjected to massive resection of the small intestine; the control group (C rats were not operated on, and soft intestinal handling was performed in sham rats. The animals were weighed weekly. On the 30th postoperative day, 0.l mL of Na99mTcO4, with mean activity of 0.66 MBq was injected intravenously into the orbital plexus. After 30 minutes, the rats were killed with an overdose of anesthetic, and fragments of the liver, spleen, pancreas, stomach, duodenum, small intestine, thyroid, lung, heart, kidney, bladder, muscle, femur and brain were harvested. The biopsies were washed with 0.9% NaCl.,The radioactivity was counted using Gama Counter WizardTM 1470, PerkinElmer. The percentage of radioactivity per gram of tissue (%ATI/g was calculated. Biopsies of the remaining jejunum were analysed by HE staining to obtain mucosal thickness. Analysis of variance (ANOVA and the Tukey test for multiple comparisons were used, considering p0.05. An increase in the weight of the SB rats was observed after the second postoperative week. The jejunal mucosal thickness of the SB rats was significantly greater than that of C and sham rats (pOBJETIVO: Avaliar em modelo animal com ressecção extensa do intestino delgado a biodistribuição de pertecnetato de sódio (Na99mTcO4 em órgãos e tecidos, a evolução ponderal e a morfometria da mucosa do intestino delgado remanescente. MÉTODOS: Vinte e um ratos Wistar foram aleatoriamente divididos em três grupos de sete animais cada. O grupo intestino curto (IC foi submetido a ressecção extensa do intestino delgado, o grupo controle (C não foi operado e o grupo sham foi

  11. Estudio mediante mecánica de fluidos computacional del efecto de la estenosis en el íleon provocada por la enfermedad de Crohn.

    OpenAIRE

    Abril López, Eduardo

    2016-01-01

    La enfermedad de Crohn es una enfermedad crónica que causa la inflamación de cualquier parte del sistema gastrointestinal, producido por el ataque al sistema inmunitario. En concreto, la parte que más suele afectarse es la parte inferior del intestino delgado (íleon) que es donde se va a centrar el estudio. Esta enfermedad de Crohn afecta al cuerpo humano inflamando el revestimiento de la parte afectada en el intestino delgado provocando una hinchazón de la pared. Si esta hinchazón (zona de l...

  12. Montelukast treatment (cysteinyl leukotriene receptor antagonist in a model of food allergy: modifications in lymphatic cell population from rectal mucosa Tratamiento con Montelukast (antagonista cisteinílico del receptor de leucotrienos en un modelo de alergia alimentaria: cambios en la población linfocítica de la mucosa renal

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    M. Vinuesa

    2010-07-01

    Full Text Available Objective: the aim is to determine immunopathological modifications in rectal mucosa from rabbits after local challenge in ovalbumin (OVA sensitized animals previously treated with montelukast. Material and methods: experimental design: thirty two rabbits divided into four groups: G1: normal; G2: subcutaneously OVA sensitized; G3: sensitized, locally OVA challenged and sampled 4 hours after challenge; and G4: sensitized, locally OVA challenged and treated 4 hours before challenge with montelukast (0.15 mg/kg. Specific anti-OVA IgE levels were evaluated by passive cutaneous anaphylaxis test (PCA. In each group 200 high microscopical power fields (HPF were counted. Results were expressed as arithmetic mean and SE. Anti -CD4, CD5, µ chain monoclonal antibodies were used. Avidin biotin horseradish peroxidase system was used. Results: CD 4: G1: 8.3 ± 0.06; G2: 13.4 ± 0.08, G3: 8.25 ± 0.06, G4: 11.8 ± 0.02. CD 5: G1: 7.3 ± 0.05; G2: 9.4 ± 0.05, G3: 11.3 ± 0.06, G4: 8.1 ± 0.06. μ chain: G1: 10.4 ± 0.06; G2: 3.8 ± 0.02, G3: 6.0 ± 0.10, G4: 2.2 ± 0.10. In all cases, experimental groups (G3 vs. G4 presented statistical significant differences (p < 0.05. CD4+, CD5+ cells and μ chain+ decrease in experimental group (G4, probably due to lymphocyte migration inhibition to challenged mucosa. μ chain+ cell decrease could be based on B cell activation and expression of different surface immunoglobulins. Cells expressing μ chain decreased in G2 and G3 likely due to activation of B cells and subsequent expression of other immunoglobulin chains in cell surface. Conclusions: we conclude that obtained data are important to elucidate immunopathology of local anaphylactic reaction in rectal mucosa from systemic sensitized animals after treatment with montelukast.

  13. Antimicrobial compounds of porcine mucosa

    Science.gov (United States)

    Kotenkova, E. A.; Lukinova, E. A.; Fedulova, L. V.

    2017-09-01

    The aim of the study was to investigate porcine oral cavity mucosa (OCM), nasal cavity mucosa (NCM), rectal mucosa (RM) and tongue mucosa (TM) as sources of antimicrobial compounds. Ultrafiltrates with MW >30 kDa, MW 5-30 kDa and MW 30 kDa, the zone of microbial growth inhibition was 7.5 mm, for the MW<5 kDa fraction, it was 7 mm, and for MW 5-30 kDa fraction, it was 4.5 mm. No significant differences were found in high molecular weight proteomic profile, while qualitative and quantitative differences were observed in the medium and low molecular weight areas, especially in OCM and NCM. HPLC showed 221 tissue-specific peptides in OCM, 156 in NCM, 225 in RM, but only 5 in TM. The results observed confirmed porcine mucous tissues as a good source of antimicrobial compounds, which could be an actual alternative for reduction of microbial spoilage of foods.

  14. Relationship of intestinal histology with the resistance to Trichostrongylus colubriformis infection in three breeds of sheep Associação entre a resposta celular no intestino e a resistência à infecção por Trichostrongylus colubriformis em três raças de ovinos

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    Alessandro F.T. Amarante

    2007-01-01

    Full Text Available The study was carried out to evaluate the relationship of inflammatory intestinal cells with the resistance to Trichostrongylus colubriformis infections in three breeds of sheep (Santa Ines, Suffolk and Ile de France, naturally infected. Mast cells, eosinophils, and globule leucocytes were enumerated in intestinal mucosa. Histamine concentration was estimated in intestinal tissue samples and the length of male and female specimens were determined. The three breeds of sheep showed similar cellular response in the small intestine mucosa (P>0.05. There was extensive variation among sheep in the parasitological and inflammatory cell variables, even in lambs of the same breed. In general, animals presenting less inflammatory cells had a larger worm burden, higher fecal egg counts, and larger T. colubriformis worms. The inflammatory cells possibly impaired the parasite's establishment, development, and survival.Avaliaram-se a associação entre o número de células inflamatórias no intestino delgado e a resistência à infecção por Trichostrongylus colubriformis em ovinos de três raças (Santa Inês, Suffolk e Ile de France, naturalmente infectados. Mastócitos, eosinófilos e leucócitos globulares foram quantificados na mucosa intestinal. A concentração de histamina foi estimada em amostras teciduais do intestino, bem como foi determinado o comprimento de machos e fêmeas de T. colubriformis. A resposta celular foi similar na mucosa intestinal das três raças ovinas (P>0,05. Houve grande variação entre os ovinos em relação aos resultados parasitológicos e celulares, mesmo nos animais de mesma raça. Em geral, os animais que apresentaram número menor de células inflamatórias tiveram cargas parasitárias maiores, contagens de ovos por grama de fezes mais altas e exemplares de T. colubriformis maiores. Os resultados indicaram que mastócitos, eosinófilos e leucócitos globulares prejudicaram o estabelecimento, o desenvolvimento e a

  15. Drug Reactions in Oral Mucosa

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    Emine Derviş

    2012-12-01

    Full Text Available Both immunologic and nonimmunologic drug reactions can be seen in oral mucosa. Since considerable number of these reactions heals spontaneously without being noticed by the patients, exact frequency of the lesions is unknown. Most common lesions are xerostomia, taste disorders, mucosal ulcerations and edema. In this article, oral lesions resulting from drug intake similar to those from oral lesions of local and systemic diseases, and diagnostic problems caused by these similarities, have been reviewed.

  16. [Redo urethroplasty with buccal mucosa].

    Science.gov (United States)

    Rosenbaum, C M; Ernst, L; Engel, O; Dahlem, R; Fisch, M; Kluth, L A

    2017-10-01

    Urethral strictures can occur on the basis of trauma, infections, iatrogenic-induced or idiopathic and have a great influence on the patient's quality of life. The current prevalence rate of male urethral strictures is 0.6% in industrialized western countries. The favored form of treatment has experienced a transition from less invasive interventions, such as urethrotomy or urethral dilatation, to more complex open surgical reconstruction. Excision and primary end-to-end anastomosis and buccal mucosa graft urethroplasty are the most frequently applied interventions with success rates of more than 80%. Risk factors for stricture recurrence after urethroplasty are penile stricture location, the length of the stricture (>4 cm) and prior repeated endoscopic therapy attempts. Radiation-induced urethral strictures also have a worse outcome. There are various therapy options in the case of stricture recurrence after a failed urethroplasty. In the case of short stricture recurrences, direct vision urethrotomy shows success rates of approximately 60%. In cases of longer or more complex stricture recurrences, redo urethroplasty should be the therapy of choice. Success rates are higher than after urethrotomy and almost comparable to those of primary urethroplasty. Patient satisfaction after redo urethroplasty is high. Primary buccal mucosa grafting involves a certain rate of oral morbidity. In cases of a redo urethroplasty with repeated buccal mucosa grafting, oral complications are only slightly higher.

  17. Effects of the combined use of glutamine and growth hormone in the intestinal adaptation after massive resection of the small bowel in rats Efeitos do uso combinado da glutamina oral e hormônio do crescimento na adaptação intestinal após ressecção extensa do intestino delgado em ratos

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    Joaquim M. Spadoni

    2005-10-01

    Full Text Available PURPOSE: The aim of this study was to investigate the effects of the combined use of glutamine (GL and growth hormone (GH in the intestine of rats submitted to 80% small bowel resection. METHODS: [24] Twenty four Wistar rats were randomized to receive either a standard rat chow - control group (CG, n=12 or the same diet added to 4% glutamine - GL-GH group (n=12 after 80% enterectomy. The latter group received subcutaneously 0,6UI/day of GH. Groups of six rats in each group were killed on the 5th and 14th days. The following variables were studied: body weight, mucosal weight, histomorphometry and DNA content in the resected specimen and in the adapted intestines after necropsy. RESULTS: All animals lost weight stabilizing after the 5th PO day in both groups. There was not any statistical difference in the mucosal weight associated to groups and dates. However, ileal mucosal weight decreased from basal to final results when compared to jejunal mucosa (p= 0.02. The DNA content increased from the initial to the final results (p OBJETIVO: Investigar os efeitos do uso combinado da glutamina (GL e do hormônio do crescimento (GH no intestino de ratos submetidos a ressecção de 80% do intestino delgado. MÉTODOS: Vinte e quatro ratos Wistar foram randomizados para receber uma a dieta padrão- grupo controle (GC, n=12 ou a mesma dieta adicionada de glutamina 4% (isocalórica, isoproteica - grupo glutamina- hormônio do crescimento (GL-GH, n=12 após a enterectomia à 80%. Este último grupo recebeu por via sub-cutânea, 0,6 UI/dia de GH. Grupos de seis ratos cada foram sacrificados no 5º e 14º dias. As seguintes variáveis foram estudadas: peso corporal, peso de mucosa, histomorfometria e conteúdo de DNA no segmento ressecado inicialmente e no intestino adaptado coletado após o sacrifício. RESULTADOS: Todos os animais perderam peso até o 5º dia, estabilizando-se após esta data em ambos os grupos. Não houve diferença estatística no peso da

  18. Estudo retrospectivo dos casos de enterolitíase e corpo estranho em intestino grosso de eqüinos, no período de janeiro de 1993 a janeiro de 2003

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    Rodrigo Romero Corrêa

    2006-04-01

    Full Text Available No período de janeiro de 1993 a janeiro de 2003, foram realizados 195 procedimentos cirúrgicos de abdômen agudo em eqüinos, no Serviço de Cirurgia de Grandes Animais do Hospital Veterinário da Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo. Dentre eles, foram diagnosticados 30 casos de enterolitíase e sete casos de corpo estranho, localizados em intestino grosso. Das 37 laparotomias, 35 foram realizadas em decúbito dorsal com acesso pela linha média e duas em estação com acesso pelo flanco. Os animais apresentaram idade variando de um a dezoito anos com média de oito anos, sendo 25 machos e 13 fêmeas. As formações e corpos estranhos apresentaram a seguinte localização: 13 (35,14% em cólon maior, 19 (51,35% em cólon menor, 03 (8,10% em cólon transverso e 02 (5,41% em cólon transverso e menor. Em relação à evolução, 23 (62,16% receberam alta e 14 (37,84% evoluíram para óbito (6 ou foram submetidos à eutanásia (8. Seis procedimentos de eutanásia foram realizados no período trans-operatório e dois no período pós-operatório. Dentre os animais que apresentaram evolução satisfatória, destacam-se um caso em que foram retirados sete enterólitos e outro em que a ponta de um prego que era o núcleo do enterólito se mantinha proeminente e em contato com a mucosa intestinal. Comparativamente às demais afecções do intestino grosso dos eqüinos e pela análise dos resultados obtidos neste estudo, conclui-se que os processos obstrutivos causados por enterólitos ou corpos estranhos apresentam prognóstico favorável.

  19. Glutamina e turnover do carbono da mucosa intestinal de leitões desmamados Glutamine and carbon turnover of the intestinal mucosa of weaned piglets

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    Fabiana Ribeiro Caldara

    2010-12-01

    Full Text Available Um experimento foi conduzido para verificar a influência da glutamina no turnover do carbono na mucosa intestinal de leitões desmamados. Nove matrizes receberam dietas compostas basicamente de plantas do ciclo fotossintético C4 durante toda a gestação (d‰13C = 17,12 e lactação (d‰13C = -19,26. Os leitões foram desmamados aos 21 dias de idade e 68 deles foram alimentados com ração composta de plantas do ciclo fotossintético C3 (d‰13C = -25,12. Os leitões foram aleatoriamente distribuídos em duas dietas: uma sem suplementação de glutamina; e outra suplementada com 1% de glutamina. Aos 0; 1; 1,5; 2; 2,5; 3; 3,5; 4; 4,5; 5; 5,5; 8; 11; 15, 20; 29 e 46 dias pós-desmame, dois leitões de cada dieta foram abatidos para coleta de amostras da mucosa intestinal, que foram analisadas quanto à composição isotópica de d‰13C e mensurada a velocidade de substituição do carbono no tempo. Os valores da meia-vida do carbono para a mucosa intestinal foram de 6,0 e 3,5 dias para as dietas controle e com glutamina, respectivamente. A glutamina acelerou a velocidade de substituição do carbono na mucosa intestinal, evidenciando sua ação benéfica na recuperação da estrutura do intestino após o desmame.An experiment was carried out to evaluate the influence of glutamine on the carbon turnover in the intestinal mucosa of weaned piglets. Nine matrices received diets predominantly composed by photosintetic cycle C4 plants during the gestation (d‰13C = -17.12 and lactation (d‰13C = -19.26. The piglets were weaned at 21 days of age, and 68 animals were fed rations composed by photosintetic cycle C3 plants (d‰13C = -25.12. The piglets were distributed at random in two treatments: one diet without glutamine supplementation and the other diet supplemented with 1% of glutamine. On days 0; 1; 1.5; 2; 2.5; 3; 3.5; 4; 4.5; 5; 5.5; 8; 11; 15, 20; 29 and 46 after weaning, two piglets from each diet were slaughtered for collection of samples

  20. Localization of ectopic gastric mucosa by scintigraphy

    International Nuclear Information System (INIS)

    D'Alonzo, W.A. Jr.

    1988-01-01

    When gastric mucosal tissue occurs outside of the confines of the stomach, it is termed ectopic or heterotopic. Ectopic gastric mucosa may be found within Meckel's diverticulum, duplications of the alimentary tract, and Barrett's esophagus. In addition, a surgeon may inadvertently leave behind antral gastric mucosa while performing a partial gastrectomy for peptic ulcer disease (i.e., retained gastric antrum). It is important to detect the presence and location of ectopic mucosa because acid and pepsin secretion may cause ulceration in the adjacent tissue resulting in serious complications. The only currently available specific diagnostic technique for detecting ectopic gastric mucosa is pertechnetate Tc 99m (TcO 4- ) scintigraphy. This chapter reviews the functional anatomy of gastric mucosa, the mechanism of TcO 4 - localization, the various entities containing ectopic gastric mucosa, and the methods and results of TcO 4 - scanning for these disorders

  1. Foreign Body in Jugal Mucosa.

    Science.gov (United States)

    Serrano, Thiago Luís Infanger; Pauna, Henrique Furlan; Hazboun, Igor Moreira; Dal Rio, Ana Cristina; Correa, Maria Elvira Pizzigatti; Nicola, Ester Maria Danielli

    2015-10-01

    Introduction Foreign body in the oral cavity may be asymptomatic for long time and only sometimes it can lead to a typical granulomatous foreign body reaction. Some patients may complain of oral pain and present signs of inflammation with purulent discharge. A granuloma is a distinct, compact microscopic structure composed of epithelioid-shaped macrophages typically surrounded by a rim of lymphocytes and filled with fibroblasts and collagen. Nowadays, the increase of cosmetic invasive procedures such as injection of prosthetic materials in lips and cheeks may lead to unusual forms of inflammatory granulomas. Objectives Describe an unusual presentation of a foreign body reaction in the buccal mucosa due to previous injection of cosmetic agent. Resumed Report A 74-year-old woman was referred to the Department of Otorhinolaryngology, Head and Neck Surgery to investigate the presence of multiple painless, bilateral nodules in the buccal mucosa, with progressive growth observed during the previous 2 months. The histologic results showed a foreign body inflammatory reaction. Conclusion Oral granulomatosis lesions represent a challenging diagnosis for clinicians and a biopsy may be necessary. Patients may feel ashamed to report previous aesthetic procedures, and the clinicians must have a proactive approach.

  2. Foreign Body in Jugal Mucosa

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    Serrano, Thiago Luís Infanger

    2015-03-01

    Full Text Available Introduction Foreign body in the oral cavity may be asymptomatic for long time and only sometimes it can lead to a typical granulomatous foreign body reaction. Some patients may complain of oral pain and present signs of inflammation with purulent discharge. A granuloma is a distinct, compact microscopic structure composed of epithelioid-shaped macrophages typically surrounded by a rim of lymphocytes and filled with fibroblasts and collagen. Nowadays, the increase of cosmetic invasive procedures such as injection of prosthetic materials in lips and cheeks may lead to unusual forms of inflammatory granulomas. Objectives Describe an unusual presentation of a foreign body reaction in the buccal mucosa due to previous injection of cosmetic agent. Resumed Report A 74-year-old woman was referred to the Department of Otorhinolaryngology, Head and Neck Surgery to investigate the presence of multiple painless, bilateral nodules in the buccal mucosa, with progressive growth observed during the previous 2 months. The histologic results showed a foreign body inflammatory reaction. Conclusion Oral granulomatosis lesions represent a challenging diagnosis for clinicians and a biopsy may be necessary. Patients may feel ashamed to report previous aesthetic procedures, and the clinicians must have a proactive approach.

  3. Morfologia macroscópica e topografia anatômica do intestino grosso da paca (Cuniculus paca Linnaeus, 1766

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    Camila Paes Burger

    2012-01-01

    Full Text Available http://dx.doi.org/10.5007/2175-7925.2012v25n2p117 O alto potencial zootécnico para a exploração da paca implica no aporte de informações sobre a morfofisiologia do trato digestório para adequada implementação do manejo nutricional. O intestino grosso da paca foi investigado em seus aspectos morfológicos e topográficos, mediante inspeção de peças a fresco e fixadas em solução aquosa de formol a 10% provindas do plantel do Setor de Animais Silvestres do Departamento de Zootecnia da Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal – UNESP (FCAV – UNESP. Este segmento, na paca, é formado pelos segmentos ceco, cólon, reto e canal anal, situa-se na cavidade abdominal e pélvica a partir das terceira ou quarta vértebras lombares. Com isso, constatou-se que, em 100% das amostras analisadas, não houve variação no padrão de disposição do intestino grosso, e que este padrão se assemelha ao dos mamíferos em geral.

  4. Effects of oral parenteral nutrition solution on the morphology and mechanical resistance of the small bowel in rats Efeitos da administração oral da nutrição parenteral total na morfologia e resistência mecância do intestino delgado, em ratos

    Directory of Open Access Journals (Sweden)

    José E. Aguilar-Nascimento

    1997-09-01

    Full Text Available The objective of this study was to investigate the effect of an elemental diet (ED on the strength and on the morphology of the small bowel. Male Wistar rats were randomized to two groups to receive during 14 days either standard laboratory rat chow (N=16 or ED (N=16 containing total parenteral nutrition (TPN solution. After this period they were killed and necropsied. The small bowel was measured and weighted with and without the contents. Bursting pressure (BP was taken from the jejunum and ileum and histological sections of these two portions was performed to register the crypt depth (CD, vilus height (VH and wall width (WW. All animals significantly gain weight. The bowel of animals fed with TPN solution had significantly less weight when compared with the controls either with (9.9 ± 1.9g x 7.8 ± 1.5g, pForam submetidos a ração padrão de laboratório 16 ratos e outros 16 receberam nutrição parenteral total (NPT por via oral, durante 14 dias. Os animais de ambos os grupos ganharam peso, sem diferença estatistica. Na necrópsia retirava-se o segmento intestinal a partir do piloro até a valva ileocecal. Não houve diferença no comprimeto do intestino delgado em ambos os grupos. Porém, o intestino dos animais alimentados com NTP apresentaram diminuiçao significante de peso comparado com o grupo controle, independentemente da presença ou não de conteúdo. A resistência intestinal era menor no grupo NTP comparado ao controle, sendo mais significante no íleo. A altura dos vilos, a profundidade das criptas e a extensão da parede eram significantemente menores no íleo no grupo NTP comparado ao controle. Este achado foi observado também no jejuno, mas sem diferença significante. Conclui-se que nos ratos alimentados com NTP o intestino delgado perde massa e torna-se menos resistente, principalmente no íleo,apesar da manutenção do estado nutricional. A perda de massa aparece não apenas na mucosa mas em toda a parede intestinal.

  5. Histologia geral dos intestinos do Caiman crocodilus yacare (Daudin, 1802 (Crocodilia: Reptilia

    Directory of Open Access Journals (Sweden)

    Sonia Maria Jin

    1990-01-01

    Full Text Available General histology of small intestine (duodenum and jejunum and the large intestine (colon and cloaca of C. c. yacare (Daudin, 1802 Crocodilia-Reptilia. A comparative description is made of the histology of the small and large intestine of C. c. yacare. In all segments mucosa, submucosa, muscularis and serosa tunics were observed. The duodenum as very long villi, wich are well-developed and attached together to form irregular transverse ridges, covered with simple columnar epithelium with marginal and goblet cells. In the jejunum, the villi are shorter and less frequent. The colon as few folds, covered with the same epithelium; the lamina própria contains straight tubular glands with absortive cells, goblet and Paneth cells. The cloaca is very complex, with three well-defined regions: coprodeum, urodeum and proctodeum. In the coprodeum, the wall is folded, less muscular and the thin mucosa is covered by an epithelium with a few basal cells and mucosal cells. The urodeum as an additional external muscular tunic, with striated muscle fibers and a stritifield cylindrical epithelium with goblet cells. The proctodeum includes the free part of the penis in the male and, laterally, the ducts of a pair of holocrine cloacal glands. Near the opening of the ducts, the epithelium changes to a squamous keratinized type.

  6. Aspectos nutricionais na população de pacientes com síndrome do intestino irritável atendidos no Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo

    OpenAIRE

    Daiana Amarante

    2013-01-01

    INTRODUÇÃO: A síndrome do intestino irritável (SII) é uma doença funcional do trato gastrintestinal que afeta até 20% da população adulta. Os principais sintomas envolvem o mau funcionamento do intestino, associados com dores abdominais, manifestação de diarreia ou constipação, sem alterações estruturais e bioquímicas do intestino. A maneira mais adequada de tratar o paciente é por meio de uma abordagem ampla e integral, porém individualizada, com identificação dos fatores desencadeantes e/ou...

  7. El Segundo Cerebro del ser humano.

    OpenAIRE

    Rocío Ponce

    2015-01-01

    Existen dos tipos de cerebros, el conocido por todos formado por el sistema nervioso central, que sería el cerebro donde las emociones se forman en base a las experiencias anteriores. El segundo cerebro, el otro, ignorado por la mayoría de seres humanos es el cerebro que está en los intestinos, Sistema Nervioso Enteral o mesentérico, que se caracteriza por la relación del cerebro y aparato digestivo, este cerebro posee más neuronas que su par, guarda emociones, determina la respuesta de acuer...

  8. Síndrome do intestino irritável e dieta com restrição de FODMAPs

    OpenAIRE

    Bastos, Tatiana Filipa dos Santos, 1992-

    2016-01-01

    Trabalho Final do Curso de Mestrado Integrado em Medicina, Faculdade de Medicina, Universidade de Lisboa, 2016 O Síndrome do Intestino Irritável (SII) é uma doença funcional do trato gastrointestinal definida de acordo com o seu padrão de sintomas (dor ou desconforto abdominal recorrente associada a alterações dos hábitos intestinais), a sua natureza crónica e ausência de doença orgânica detetável. Embora a SII não constitui uma condição com risco de vida, tem uma prevalência elevada e red...

  9. Úlcera eosinófila de la mucosa oral Eosinophilic ulcer of oral mucosa

    Directory of Open Access Journals (Sweden)

    A.C. Bencini

    2009-08-01

    Full Text Available La Úlcera Eosinófila de la Mucosa Oral, es una entidad poco frecuente, pobremente descrita en la literatura mundial. Se define como una lesión benigna autolimitada que si bien puede presentarse en distintas áreas de la cavidad bucal, presenta una marcada predilección por la mucosa ventral de la lengua. Clínicamente, se presenta como una lesión ulcerada de bordes indurados y sobreelevados. Los hallazgos histopatológicos son característicos y consisten en un infiltrado mixto rico en eosinófilos, acompañado de una población de grandes células mononucleadas. Recientes artículos basados en estudios inmunohistoquimicos, permiten afirmar la presencia de grandes linfocitos atípicos CD30+ y por lo tanto, incluir esta lesión en el espectro de las entidades simuladoras de desordenes linfoproliferativos. A pesar de esto, el mecanismo etiopatogenico permanece oscuro y el trauma local juega un rol todavía no dilucidado; aunque se halla presente en la mayoría de las publicaciones, explicando el fenómeno como un mecanismo reactivo. La importancia de esta lesión, radica en su diagnostico diferencial por su semejanza clínica al carcinoma espinocelular, histoplasmosis, chancro sifilítico, Úlcera tuberculosa, carcinoma epidermoide y otras. En nuestro trabajo se revisa la literatura y se discuten la características clínicas, histopatológicas y alternativas terapéuticas, a partir del artículo de un caso clínico en una paciente joven, que luego de la biopsia escisión como método para el diagnostico de certeza, se produce una recidiva de la lesión; lo que orientó el tratamiento hacia la cirugía combinada con corticoterapia local intralesional, logrando su remisión.Eosinophilic Ulcer of the Oral Mucosa, an entity, poorly deciphers in world-wide literature. It is defined as a self-limited, benign injury that although it can appear in different areas of the buccal cavity it presents a noticeable predilection via the ventral mucosa of the

  10. Endoscopic appearance of irradiated gastric mucosa

    Energy Technology Data Exchange (ETDEWEB)

    De Sagher, L I; Van den Heule, B; Van Houtte, P; Engelholm, L; Balikdjan, D; Bleiberg, H

    1979-09-01

    Irradiation of the epigastric area for gastric cancer may induce actinic lesions of the stomach characterized on endoscopic examination by ulcerations, haemorrhagic gastritis, fragility of the mucosa, thickening and congestion of the gastric folds.

  11. Oral mucosa grafts for urethral reconstruction

    African Journals Online (AJOL)

    reports reveal that split and full thickness skin grafts from the scrotum, penis, extragenital sites (ureter, saphenous .... Table 1: Summary of the history of oral mucosa grafts for urethroplasty .... advised that care should be taken when suturing the.

  12. IMMUNOHISTOCHEMICAL ANALYSIS OF ORAL MUCOSA LEUKOPLAKIA

    Directory of Open Access Journals (Sweden)

    Yu. G. KOLENKO

    2016-06-01

    Full Text Available In recent years, substantial changes have occurred in the structure of oral mucosa diseases, in particular an increased ratio of precancerous diseases, so that an effective non-invasive detection of any sign of malignancy appears as an urgent and most actual task of dentistry. Aim: To study the proliferative activity of epithelial cells in Ki-67 antigenin patients with leukoplakia of the oral mucosa. Materials and method: A complex clinical and laboratory examination was performed on 155 patients with oral leukoplakia, who addressed the Operative Dentistry Department of the “A.A.Bogomolets” National Medical University of Kiev between 2010 and 2014. All patients have been subjected to a careful clinical examination, which included: dental anamnesis, visual inspection, oral examination and digital palpation of oral mucosa and tongue mucosa, biopsy of leukoplakia lesions for cytological and histological examination. Results: Histological evaluation of the material has been performed according to the WHO (2005 classification of leukoplakia. 10 (14% sites of unaltered mucosa, 10 (14% samples of hyperkeratosis without atypia, 14 (19% biopsy specimens of hyperkeratosis SIN1, 15 (21% – hyperkeratosis SIN2, 10 (14% - SIN3 and 13 (18% cases of squamous cell carcinoma were evidenced. Immunohistochemical investigation evidenced the presence of protein Ki-67 in the nuclei of epithelial cells. In the unmodified epithelium of the oral mucosa, all epithelial cells with stained nuclei are virtually located in the basal layer. Conclusion: Against the general increase of the proliferative activity of epithelial cells with increasing SIN, a characteristic distribution of proliferating cells in the thickness of the epithelium was revealed for each studied group, as follows: in the control group and in leukoplakia without atypia, immunopositive cells are located in the basal layer, in leukoplakia (SIN1, SIN2 and SIN3 – in parabasal position while, in squamous

  13. Clinical use of growth hormone and glutamine in short bowel syndrome Uso clínico de hormônio de crescimento e glutamina em síndrome do intestino curto

    Directory of Open Access Journals (Sweden)

    Celso Cukier

    1999-02-01

    Full Text Available Growth hormone (GH and glutamine (GLN are considered bowel trophic factors and are used experimentally after bowel resection. Their clinical uses in short bowel syndrome (SBS are still not standardized. It is of interest to verify metabolic, nutritional and side effects of the association of GH and GLN in SBS. Three patients, 39 (A, 33 (B, and 01 years old (C underwent bowel resection with jejunum anastomosis 15 cm (A and 60 cm (B distant from the Treitz angle, and 40 cm (C preserving the ileo cecal valve. GH Saizen (Serono - A, Genotropin (Pharmacia - B, and Norditropin (Novonordisk C were administered in doses of 0.14 mg /kg/day. GLN (0.4 g/kg/day was given orally for 10 days (A, 30 days (B and 60 days to patient C (0.28 g/kg/day. Central TPN and adequate oral diet was administered according to the bowel adaptation phase. On the first day after beginning treatment patient A exhibited symptoms of hypoglycemia. There were no other side effects. After treatment, body weight was higher and analysis by bioelectrical impedance showed more lean mass and less fat mass compared to pre-treatment measurements. Nitrogen retention was progressively higher with treatment. Simultaneous treatment with GH and GLN does not cause significant side effects, and is associated with a favorable distribution of the body compartments and nitrogen retention in patients with the short bowel syndrome.Hormônio de crescimento (GH e glutamina (GLN são fatores tróficos da mucosa intestinal, utilizados experimentalmente pós ressecção intestinal maciça. O uso clinico de GH e GLN em síndrome do intestino curto (SIC ainda não está padronizado. Torna-se de interesse verificar os efeitos metabólicos, nutricionais e colaterais da associação do GH e GLN em SIC grave de origem recente. Três pacientes, com idades de 39 anos (A, 33 anos (B e 1 ano (C foram submetidos à enterectomia com anastomose do jejuno a 15 cm (A e 60 cm (B do angulo de Treitz com o cólon ascendente

  14. [Nasal mucosa in patients with diabetes mellitus].

    Science.gov (United States)

    Müller, Maciej; Betlejewski, Stanisław

    2003-01-01

    Diabetes mellitus is the most common endocrinologic disease all over the world. 150 million people suffer from this disease, in Poland about 2 million. The disease on the basis of the onset and pathophysiology may be divided into type I and type II. Pathophysiologic changes include diabetic microangiopathy, macroangiopathy and neuropathy. The most common presentations in head and neck are otitis externa, hypoacusis, vertigo, disequilibrium, xerostomia, dysphagia, fungal and recurrent infections. The changes in nasal mucosa are not very well known. Only few papers concerned the problem. The main complaints of patients regarding the nose are xeromycteria, hyposmia and various degree of decreased patency of the nose. Chronic atrophic rhinitis, septal perforation, ulceration of nasal mucosa, alar necrosis, symptoms of staphylococcal or fungal infection can be found during otolaryngologic examination. The treatment in this group of patients should consist of systemic therapy of diabetes mellitus and on the other hand focal therapy with the use of a solution to moisten the nasal mucosa.

  15. Radiation tolerance of the vaginal mucosa

    International Nuclear Information System (INIS)

    Hintz, b.L.; Kagan, A.R.; Chan, P.; Gilbert, H.A.; Nussbaum, H.; Rao, A.R.; Wollin, M.

    1980-01-01

    Sixteen patients with cancer of the vagina that were controlled locally for a minimum of eighteen months after teletherpay (T) or brachytherapy (B) or both (T and B), were analyzed for radiation tolerance of the vaginal mucosa. The site of vaginal necrosis did not always coincide with the site of the tumor. The posterior wall appeared more vulnerable than the anterior or lateral walls. For the distal vaginal mucosa, necrosis requiring surgical intervention occurred following combined T and B, if summated rad exceeded9800. The upper vagina tolerated higher dosages. No patient surgery for upper vaginal necrosis even though summated (T and B) dosage up to 14,000 rad was applied. Placing radioactive needles on the surface of the vaginal cylinder with or without interstitial perincal needles should be avoided. Further accumulation of data is needed to define these vaginal mucosa tolerance limits more closely

  16. [Effect of nasogastric tube on esophageal mucosa].

    Science.gov (United States)

    Barinagarrementeria, R; Blancas Valencia, J M; Teramoto Matsubara, O; de la Garza González, S

    1991-01-01

    We studied 30 patients. 20 were males and 10 females. Mean age was 48 year old. Esophageal disease was not present neither gastro-esophageal reflux. Biopsy was taken between 24 hours and 25 days after nasogastric tube (NG) was put into place. Endoscopic findings were: hyperemic mucosa, submucosal hemorrhage, clots, erosions and ulcers near Esophago-gastric junction. Intraepithelial edema, vessel congestion, polymorphonuclear infiltration, fibrin thrombosis of submucosal vessels, ischemia, epithelial regeneration and ulcer were common histologic findings. All endoscopic and histologic alterations were related to the length of time of NG tube contact with the esophageal mucosa. We concluded that NG tube damages the esophageal mucosa by two mechanisms: a) Local irritation that favors b) gastric reflux by decreasing lower esophageal sphincter pressure.

  17. Trasplante de mucosa oral en la reconstrucción de las vías lagrimales Oral mucosa transplants in restoration of lacrimal canaliculi

    Directory of Open Access Journals (Sweden)

    Bernardo Canto Vidal

    2000-06-01

    Full Text Available Se realizaron 143 intervenciones de las vías lagrimales, mediante la técnica quirúrgica de trasplante de mucosa oral con 55 injertos libres y 88 tubulares, a partir del año 1995. Para ello se tuvo en cuenta el examen clínico y radiológico y el tipo de afectación. Con la utilización de las técnicas de injerto libre o tubular de la mucosa se puede restablecer la fisiología lagrimal en los pacientes con mutilaciones por traumatismos o presencia de tumores con disminución del riesgo quirúrgico; además se recomienda por novedosa, práctica y por los buenos resultados obtenidos.143 interventions of lacrimal canaliculi were carried out, throgh surgical technique of oral mucosa transplant (55 free grafts and 88 tutular ones since 1995, bearing in min radiological and clinical examination and type of invelvement. Using technique of free or tubular graft, it is possible restore lacrimal physiology in patients with mutilations from trauma or prevence of tumors thus decreasing surgical risk; this tachnique is recommended by its novelty, usefulness . and by good results obtained.

  18. Proteome Analysis of Rheumatoid Arthritis Gut Mucosa

    DEFF Research Database (Denmark)

    Bennike, Tue Bjerg; Ellingsen, Torkell; Glerup, Henning

    2017-01-01

    Rheumatoid arthritis (RA) is an inflammatory joint disease leading to cartilage damage and ultimately impaired joint function. To gain new insight into the systemic immune manifestations of RA, we characterized the colon mucosa proteome from 11 RA-patients and 10 healthy controls. The biopsies were...

  19. Ectopic gastric mucosa in the duodenal bulb

    International Nuclear Information System (INIS)

    Schnell, H.; Oehler, G.; Schulz, A.; Rau, W.S.; Giessen Univ.; Giessen Univ.

    1989-01-01

    The radiological and clinical findings of 12 patients with ectopic gastric mucosa in the duodenal bulb are presented. This is a defined disease with characteristic radiological features: multiple small nodular defects of the contrast medium of 1-3 mm diameter. Histology shows complete heterotopia. Pathogenesis and clinical significance are discussed with reference to the literature on this subject. (orig.) [de

  20. Esophageal heterotopic gastric mucosa in esophageal atresia

    Directory of Open Access Journals (Sweden)

    Lachlan J.R. Harrison

    2018-05-01

    Full Text Available Heterotopic gastric mucosa (HGM is occasionally found at endoscopy in the proximal esophagus of adults and children, when it manifests as an asymptomatic small island of reddish pink mucosa just below the upper esophageal sphincter. There are few reports of esophageal HGM detected by endoscopy after repair of esophageal atresia (EA with tracheo-esophageal fistula (TEF. We report a child with multiple patches of HGM in the proximal and distal esophagus seen at endoscopy after EA/TEF repair. No obvious symptoms were related to the HGM and she remains under endoscopic surveillance. The incidence of esophageal HGM may be increased in patients with EA and its distribution can be more extensive than a simple “inlet patch”. There is evidence to suggest that esophageal HGM increases the risk of developing Barrett's esophagus and has a malignant potential. Heterotopic gastric mucosa extends the spectrum of potential pathologies affecting the esophagus in patients with EA/TEF and supports current international guidelines for endoscopic surveillance of these patients. Keywords: Tracheo-esophageal fistula, Ectopic mucosa, Esophageal malignancy

  1. Análise da comunicação não-verbal de pessoas portadoras de ostomia por câncer de intestino em grupo focal Analisis de la comunicación no verbal de personas com ostoma por cáncer de intestino en un grupo focal Analysis of nonverbal communication of ostomy patients due to bowel cancer in a focus group

    Directory of Open Access Journals (Sweden)

    Silene Oliveira Paegle

    2005-02-01

    Full Text Available O objetivo foi analisar a freqüência de comportamentos eficazes e ineficazes nas relações de pessoas portadoras de ostomia por câncer de intestino, integrantes de um grupo. Caracterizou-se por ser de campo, exploratório, observacional, realizado num hospital da cidade de São Paulo. A população foi composta por 5 pessoas reunidas em grupo. Após aprovação pelo Comitê de Ética institucional, ocorreram 5 encontros norteados pelos temas: eu antes do adoecimento, eu doente e o tratamento, eu me recuperando com uma ostomia, eu e minha vida cotidiana, eu neste grupo, durante os meses de outubro e novembro de 2001. Os dados foram coletados por meio de gravação em vídeo, durante a realização dos encontros, e registro de diário de campo. A análise quantitativa foi realizada com base na confecção de tabelas e gráfico, a partir do modelo estabelecido. Explicitou-se predomínio de rigidez e competitividade nas relações intragrupais, determinando paralisia e estereotipia na interação.La finalidad de este estudio fue analizar la comunicación no verbal de portadoras de ostoma por cáncer de intestino, integrantes de un grupo. Fue realizada una investigación de campo, de tipo exploratorio y observacional, en un hospital de la ciudad de São Paulo, Brasil. La población estuvo compuesta de 5 personas. Tras la aprobación por el Comité de Ética institucional, ocurrieron 5 encuentros durante los meses de octubre y noviembre del 2001, norteados por los temas: yo antes de adolecer, yo enfermo y el tratamiento, yo recuperándome con una ostomia, yo y mi vida cotidiana, yo en este grupo. Los datos fueron recolectados por medio de grabación en video, durante la realización de los encuentros, y se efectuó el registro de diario de campo. El análisis cuantitativo fue realizado basado en la confección de tablas y gráficos, a partir del modelo establecido. Se explicitó predominio de rigidez y competitividad en las relaciones

  2. Papel del estrés en la intensidad de los síntomas SII

    OpenAIRE

    Pletikosic, Sanda; Tkalčić, Mladenka

    2016-01-01

    Síndrome del intestino irritable se considera un trastorno biopsicosocial, el resultado de la combinación compleja de factores predisponentes, precipitantes y perpetuos. Rasgos de personalidad, estado afectivo y estrés son algunos de los factores relevantes que contribuyen a la calidad de vida más baja y la exacerbación sintomática de los pacientes con SII. Para examinar el papel del estrés en la exacerbación sintomática de SII, el objetivo de este estudio era explorar la relación entre los a...

  3. Efficacy of radiotherapy of oral mucosa cancer

    International Nuclear Information System (INIS)

    Vorob'ev, Yu.I.; Garbuzov, M.I.; Sarantseva, I.P.; Popov, N.V.; Pereslegin, O.I.

    1986-01-01

    An analysis of 10-year experience of a radiological department (962 patients) indicated late admission of oral mucosa cancer patients for specialized treatment: 75-85% of the patients were admitted with Stage 2-4 disease. The assessment of the efficacy of radiotherapy according to the 3 ad 5-year survival rates showed that better results were obtained for buccal mucosa cancer and the worst for mouth fundus cancer. Regional metastates are a poor prognostic sign, particularly fixed metastases in patients with tongue and mouth fundus cancer. Combined therapy turned out be the most effective in tongue cancer. In different variants of dose delivery in time the most favorable results were obtained with small fractionation (a conventional course). However it should be noted that a split course was usually applied to weak elderly patients with advanced stages of disease

  4. Oral Neurothekeoma of the Right Buccal Mucosa

    Directory of Open Access Journals (Sweden)

    Alex C. Tham

    2016-01-01

    Full Text Available Oral neurothekeoma or nerve sheath myxoma is a rare benign oral tumour of nerve sheath origin. Historically, this tumour has been subclassified as myxoid (classic, mixed, or the cellular type, depending on the amount of myxoid stroma and cellularity. We present a case of oral neurothekeoma (mixed type of the buccal mucosa. The tumour was completely excised. No recurrence was detected in the last 3 years after local excision.

  5. Dopamine receptors in human gastrointestinal mucosa

    International Nuclear Information System (INIS)

    Hernandez, D.E.; Mason, G.A.; Walker, C.H.; Valenzuela, J.E.

    1987-01-01

    Dopamine is a putative enteric neurotransmitter that has been implicated in exocrine secretory and motility functions of the gastrointestinal tract of several mammalian species including man. This study was designed to determine the presence of dopamine binding sites in human gastric and duodenal mucosa and to describe certain biochemical characteristics of these enteric receptor sites. The binding assay was performed in triplicate with tissue homogenates obtained from healthy volunteers of both sexes using 3 H-dopamine as a ligand. The extent of nonspecific binding was determined in the presence of a 100-fold excess of unlabeled dopamine. Scatchard analysis performed with increasing concentrations of 3 H-dopamine (20-500 nM) revealed a single class of saturable dopamine binding sites in gastric and duodenal mucosa. The results of this report demonstrate the presence of specific dopamine receptors in human gastric and duodenal mucosa. These biochemical data suggest that molecular abnormalities of these receptor sites may be operative in the pathogenesis of important gastrointestinal disorders. 33 references, 2 figures

  6. A problemática da deiscência de anastomoses no intestino grosso em canídeos : a propósito de um caso clínico

    OpenAIRE

    Correia, Ana Sofia Lopes

    2015-01-01

    Dissertação de Mestrado Integrado em Medicina Veterinária De etiologia não totalmente elucidada, a deiscência de anastomose no intestino grosso é uma complicação pós-operatória potencialmente fatal, que ocorre mais frequentemente entre o 3º e o 5º dia pós-operatórios. Poucos são os estudos sobre este assunto em medicina veterinária. O objetivo desta dissertação foi apresentar e discutir os fatores de risco associados à deiscência de anastomose no intestino grosso considerando duas localiza...

  7. Usefulness of capsule endoscopy in patients with suspected small bowel disease Utilidad de la cápsula endoscópica en pacientes con sospecha de patología de intestino delgado

    Directory of Open Access Journals (Sweden)

    A. Caunedo

    2004-01-01

    digestiva de origen oscuro o anemia ferropénica (n = 13; molestias abdominales en pacientes consumidores de AINE (n = 7; estudio de extensión de tumores gastrointestinales (n = 4; y controles asintomáticos (n = 2. Todos los pacientes fueron sometidos previamente a tránsito intestinal, gastroscopia y colonoscopia, cuyos hallazgos no fueron concluyentes para el diagnóstico. Resultados: los hallazgos relevantes más frecuentemente encontrados fueron ulceraciones y aftas yeyunoileales (29 pacientes, malformaciones vasculares (13 pacientes y neoplasias intestinales (6 pacientes. Los grupos con mayor tasa de hallazgos relacionados con la indicación fueron el de hemorragia digestiva de origen oscuro (76,92% y el de diarrea crónica (67,85%, mostrando el grupo de pacientes con dolor abdominal el porcentaje más bajo (34,48%. En el 40,90% de casos (36/88 se cambió directamente la estrategia terapéutica del paciente a la luz de los resultados capsuloendoscópicos. La única complicación observada consistió en la falta de excreción de una de las cápsulas por la existencia de una estenosis ulcerada no conocida. Conclusiones: la cápsula endoscópica es una técnica segura y capaz de estudiar el intestino delgado en su totalidad, suponiendo una valiosa herramienta para el manejo de pacientes con clínica sugestiva de patología intestinal.

  8. Fontes proteicas e idades de abate sobre a estrutura e ultraestrutura do intestino delgado de leitões desmamados

    Directory of Open Access Journals (Sweden)

    Maria Cristina Thomaz

    2011-10-01

    Full Text Available Foram realizados dois experimentos (experimento 1 - alimentação convencional e experimento 2 - alimentação das matrizes, a partir de 109 dias de gestação, na lactação e dos leitões lactentes com ração sem farelo de soja, com o objetivo de avaliar os efeitos de fontes proteicas (farelo de soja - FS, soja semi-integral extrusada - SSIE e produtos lácteos - PL sobre a morfologia intestinal de leitões abatidos aos 0,5 e 15 dias após a desmama. Foram utilizados 27 leitões, desmamados aos 25 dias de idade, sendo que em cada idade, nove leitões foram abatidos. Utilizou-se o delineamento em blocos ao acaso, em esquema fatorial 3 x 3 (três dietas x três idades de abate. Os resultados demonstraram que os animais recebendo FS apresentaram menores alturas das vilosidades e relação vilosidade:cripta, quando comparados àqueles que receberam SSIE ou PL, não havendo, entretanto, diferenças entre estes. A altura da vilosidade e a relação vilosidade:cripta não foram afetadas pela alimentação diferenciada das matrizes e dos leitões antes da desmama. A profundidade das criptas não foi afetada pelas dietas ou idades de abate, dentro de cada experimento, em nenhum dos segmentos estudados, sendo, no entanto, menor no duodeno e maior no jejuno e íleo dos animais do exp. 2, quando comparados àqueles do exp. 1. Concluiu-se que o FS afetou negativamente a altura das vilosidades e a relação vilosidade:cripta após a desmama; que a morfologia intestinal foi mais afetada aos cinco dias, mostrando sinais de recuperação aos 15 dias após a desmama e que a alimentação das matrizes não afetou a morfologia dos segmentos do intestino delgado.

  9. Síndrome do intestino curto: uma nova alternativa de tratamento cirúrgico Short-bowel syndrome: a new alternative for surgical treatment

    Directory of Open Access Journals (Sweden)

    Orli Franzon

    2010-03-01

    Full Text Available INTRODUÇÃO: A síndrome do intestino curto resulta de comprimento total de intestino delgado inadequado para manter a nutrição, e assim, os pacientes tornam-se cronicamente dependentes de nutrição parenteral total. Numerosas estratégias cirúrgicas foram estudadas para restabelecer a função intestinal normal. OBJETIVO: Propor a utilização da inversão de segmento de alça associada à STEP - Serial Transverse Enteroplasty - para aumentar a área absortiva intestinal em pacientes com síndrome do intestino curto. MÉTODO: Os autores descrevem uma nova alternativa para o tratamento cirúrgico da síndrome do intestino curto, demonstrando sua facilidade e eficiência para o objetivo proposto. RESULTADOS: As duas técnicas associadas permitiram alongar a alça intestinal em 2,6 vezes do comprimento original, com posterior ganho de peso, redução do número de evacuações para uma exoneração ao dia e melhora da qualidade de vida social e psicológica. CONCLUSÃO: Essa técnica de inversão de segmento de alça intestinal e posterior STEP surge como tratamento eficiente e definitivo à síndrome do intestino curto de forma promissora e segura permitindo retorno à alimentação por via oral com satisfatória absorção de nutrientes e ganho de peso, retorno da motilidade efetiva intestinal, associada à baixa morbidade e melhoria da qualidade de vida social e psicológica. Apesar de requerer várias cargas de grampeadores lineares apresenta baixa morbidade e passa a tornar-se um potencial substituto para o transplante intestinal.BACKGROUND: The short bowel syndrome results of inadequate total length of small intestine that is not enough to nutrition, and so, the patients become dependents of total parenteral nutrition. Numerous surgical strategies had been studied to reestablish the normal intestinal function. OBJECTIVE: Consider the inversion segment of small intestine associated with STEP - Serial Transverse Enteroplasty - to increase

  10. Premalignant alterations of the gastric mucosa

    International Nuclear Information System (INIS)

    Frager, D.; Mitsudo, S.; Kozecky, O.; Frager, J.; Wolf, E.; Beneventano, T.C.

    1986-01-01

    Atrophic gastritus or intestinal metaplasia is the precursor to many gastric carcinomas that arise in a dysplatic epithelium. The authors retrospectively reviewed the radiographic features of the gastric mucosa in 30 patients with the pathologic diagnosis of intestinal metaplasia (27) or atrophic gastritus (3). In 12 patients (40%) the area gastricae were enlarged to 5 mm or greater. In these 12 patients and in an additional 11 (total of 23, or 76%), a polypoid-nodular gastric mucosal pattern was seen. These findings and patterns are illustrated, and the differential diagnosis and clinical implications are discussed

  11. CYTOKINE REGULATION OF ULCEROGENESIS IN GASTRODUODENAL MUCOSA

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    L. V. Matveeva

    2013-01-01

    Full Text Available Ulcerogenesis in gastroduodenal mucosa area is a complex multistep process. Its, phases arecontrolled by interaction and activation of pro­ and antiinflammatory cytokine cascade. Present review article summarizes scientific data on impact of cytokines upon ulcerative and reparatory processes, a variety of their diagnostic and therapeutic options is defined. Evaluation of cytokine status, or, in some cases, cytokine genotyping in patients with stomach and duodenal ulcers, may predict clinical course of the disease, as well as efficiency of basic and eradication therapy, correction of the treatment.

  12. Descripción histológica comparativa del desarrollo del sistema digestivo y visual de larvas de chame Dormitator latifrons (Pisces: Eleotridae

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    Vanesa V López-López

    2015-07-01

    Full Text Available Se realizó la descripción del desarrollo de larvas de uno a seis días post-eclosión (dpe, para generar información que ayude a comprender la capacidad alimenticia en etapas tempranas. Las larvas se obtuvieron mediante la inducción hormonal de reproductores, utilizando implantes de análogos sintéticos de GnRH durante la temporada de septiembre-noviembre de 2010. Las muestras fueron incluídas en historesina y teñidas con hematoxilina-eosina. Una vez eclosionada, la larva presenta gran cantidad de vitelo que absorbe gradualmente (1° a 3° dpe. Al 4° dpe, existe una cavidad bucal abierta con estructuras dentales, en este estadio se ha completado el desarrollo ocular, que presenta pigmentación. Se observa la formación de las hendiduras branquiales y se diferencia el intestino en anterior y posterior y se distingue la presencia de vacuolas digestivas en el intestino anterior. En el 5° dpe, se observaron los paquetes de fibras musculares y se distinguió una estructura estomacal. En el 6° dpe, el páncreas, pliegues intestinales y membrana de borde de cepillo son visibles y se observaron partículas de alimento y bacterias dentro del intestino. En base a lo anterior, se concluye que las larvas de D. latifrons han desarrollado completamente el tracto digestivo y órganos asociados que le ayudarán a la búsqueda de alimento, entonces a partir del 4° dpe son capaces de iniciar la alimentación exógena.

  13. Honey and Apoptosis in Human Gastric Mucosa

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    Alireza Ostadrahimi

    2012-07-01

    Full Text Available Background: Gastric cancer is the fourth most common malignancy in the world. Honey is acomplex mixture of special biological active constituents. Honey possesses antioxidant and antitumorproperties. Nutritional studies have indicated that consumption of honey modulates therisk of developing gastric cancer. On the other hand, apoptosis has been reported to play a decisiverole in precancerous changes. Our chief study was conducted to assess the relationship betweenconsumption of honey and apoptosis in human gastric mucosa.Method: This cross-sectional study was conducted on 98 subjects over 18 years old, referred totwo hospitals in Tabriz, Iran. Subjects were undergone an upper gastrointestinal endoscopy, 62subjects were finally enrolled. Honey consumption was assessed by a Food Frequency Questionnaire(FFQ and apoptosis was detected by TUNEL technique. We tested polynomial curve tofind the best fit between honey consumption and apoptosis.Results: A positive relation between honey consumption and apoptosis was found (P=0.024.Our results indicated that the final and the best fit curve was: apoptosis = 1.714+1.648(honeyamount - 0.533(honey amount2 +1.833×10-5(honey amount7.Conclusion: Honey consumption had positive effects on gastric cancer by inducing apoptosis ingastric mucosa.

  14. Cholesterol esterase activity of human intestinal mucosa

    International Nuclear Information System (INIS)

    Ponz de Leon, M.; Carubbi, F.; Di Donato, P.; Carulli, N.

    1985-01-01

    It has been suggested that cholesterol absorption in humans is dependent on bile acid pool composition and that expansion of the cholic acid pool size is followed by an increase of the absorption values. Similar observations were reported in rats. In the present study, therefore, the authors investigated some general properties of human intestinal cholesterol esterase, with particular emphasis on the effect of bile acids on this enzymatic activity. Twenty-nine segments of small intestine were taken during operations; the enzymatic activity was studied by using mucosal homogenate as a source of enzyme and oleic acid, cholesterol, and 14 C-labeled cholesterol as substrates. The time-activity relationship was linear within the first two hours; optimal pH for esterification ranged between 5 and 6.2. There was little difference between the esterifying activity of the jejunal and ileal mucosa. Esterification of cholesterol was observed with all the investigated fatty acids but was maximal with oleic acid. Bile acids did not affect cholesterol esterase activity when present in the incubation mixture at 0.1 and 1.0 mM; the enzymatic activity, however, was significantly inhibited when bile acids were added at 20 mM. In conclusion, this study has shown that the human intestinal mucosa possesses a cholesterol esterase activity; at variance with the rat, however, the human enzyme does not seem to be stimulated by trihydroxy bile acids

  15. Evaluation of pemphigus cases involving oral mucosa.

    Science.gov (United States)

    Karagöz, Gizem; Bektaş-Kayhan, Kivanç; Ünür, Meral

    2014-09-01

    Pemphigus, defines a group of disorders in autoimmune etiology which could be life-threatening and clinical manifestations are mainly epithelial blistering affecting cutaneous and/or mucosal surfaces including oral mucosa. The aim of our study is to evaluate the clinical appearance of pemphigus with oral involvement by reported 15 pemphigus cases. This retrospective study of 15 cases of pemphigus obtained over a period of 7 years from 2006 to 2013 in Istanbul University, Dentistry Faculty, Oral Medicine and Surgery Department was designed. Age distribution of pemphigus was from 15 to 59 years with an average age of 41.3 years. Of the 15 patients, the male:female ratio was 1:2.75 (4 male, 11 female). The most common clinical various of pemphigus was pemphigus vulgaris, diagnosed in all patients. The buccal mucosa (34.3%) was the most commonly affected site followed by tongue (20%), gingiva (17.1%), palate (11.4%), lips (11.4%) and floor of mouth (5.7%). Our explanation of this conclusion, while speculative, is that socioeconomic situation related stress in males and hormonal changes like pregnancy and menostasis in females; systemic disease and using drugs; dental trauma and bruxism could be responsible for flare up in the disease.

  16. Atrofia mucosa/translocação bacteriana na sepse experimental em ratos Wistar Mucosal atrophy/bacterial translocation in experimental sepsis in Wistar rats

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    Armando José d'Acampora

    2004-10-01

    Full Text Available OBJETIVO: Avalizar a relação entre lesão mucosa e translocação bacteriana. MÉTODO: Utilizou-se 50 ratos distribuídos em 5 grupos: 1.Controle: injeção de inóculo padrão de Pseudomonas aeruginosa, 2.Dreno: injeção do inóculo padrão e drenagem da cavidade abdominal, após 6 horas, 3.Lavado: injeção do inóculo padrão e lavagem da cavidade abdominal, após 6 horas, 4.Lavado + dreno: injeção do inóculo padrão e após 6 horas, drenagem e lavagem da cavidade, 5.Normal: avaliação histológica da parede intestinal normal. Após o óbito, realizou-se hemocultura e cultura peritoneal. Realizou-se medida da espessura total da parede do jejuno e da camada mucosa em vilosidades seccionadas de forma longitudinal. RESULTADOS: Na hemocultura, houve crescimento de Pseudomonas aeruginosa e Escherichia coli em 90% e 52,5% dos animais. Na cultura peritoneal, houve crescimento de P. aeruginosa, E. coli e Klebsiella sp em 87,5%, 85% e 5% dos animais. Quanto a altura da camada mucosa e da parede intestinal, não houve alteração estatisticamente significativa entre os 5 grupos. CONCLUSÃO: A sepse aguda não causou alteração na camada mucosa do intestino delgado e a translocação ocorrida não pode ser considerada como decorrente de uma lesão da mucosa intestinal.PURPOSE: Observe the relation between small intestine's mucosal injury and bacterial translocation. METHODS: 50 adult female rats were distributed in 5 groups: 1. Control: intraperitoneal injection of Pseudomonas aeruginosa, 2. Drainage: intraperitoneal injection of P. aeruginosa and drainage of the abdominal cavity, after 6 hours, 3. Washed: intraperitoneal injection of P. aeruginosa and washing of the abdominal cavity, after 6 hours, 4. Washed + drainage: intraperitoneal injection of P. aeruginosa plus drainage and washing of the cavity, after 6 hours, 5. Normal: evaluation of the normal intestinal wall. After death, blood and peritoneal cultures were performed. Fragments of

  17. ESTUDO DA MUCOSA ENTERAL DE RESERVATÓRIOS EM "J" EM CÃES

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    SANDRA PEDROSO DE MORAES

    1998-07-01

    Full Text Available A proctocolectomia total com reservatório enteral é a principal técnica utilizada para o tratamento operatório de algumas doenças do intestino grosso. Com o objetivo de estudar as características da mucosa enteral de reservatórios em J, foram operados 36 cães. Após a ressecção de 5 cm do jejunum caudalis, para estudo-controle, os cães foram separados em dois grupos (GI e GII. No GI, foi realizada anastomose êntero-enteral término-terminal; no GII, foi feito um reservatório na forma de "J" no segmento cranialis, que foi anastomosado ao segmento caudalis da mesma forma que no GI. Aos 21, 42 e 63 dias de pós-operatório, foi realizada eutanásia de 6 cães de cada grupo para estudos macroscópico e microscópico, incluindo-se contagens celulares e medidas da lâmina própria da mucosa enteral. Os resultados revelaram, aos 21 dias, edema e enantema, à macroscopia, e necrose à microscopia, em freqüências semelhantes nos dois grupos. Aos 42 e 63 dias, essas alterações diminuiram ou desapareceram no GI e persistiram no GII. Somente neste último foram observadas úlceras à macroscopia aos 21 dias (2 cães e 42 dias (1 cão. Não houve diferença significante à presença de abscesso, à microscopia, nos dois grupos. Na contagem celular, já aos 21 dias houve aumento da densidade volumétrica de neutrófilos, macrófagos, eosinófilos, plasmócitos e linfócitos nos dois grupos. Todas as células, exceto eosinófilos, apresentavam-se com densidade volumétrica significantemente maior no GII, aos 63 dias. O diâmetro transversal da lâmina própria, excluindo a altura dos vilos, apresentou-se significantemente maior no GII aos 63 dias de pós-operatório. Concluiu-se que a mucosa enteral de reservatórios em J, em cães, apresenta modificações do padrão de normalidade macroscópica e microscópica, entre as quais o aumento da densidade volumétrica de células inflamatórias.Total proctocolectomy with enteral reservoir is, at

  18. Absorção de anticorpos do colostro em bezerros: I. Estudo no intestino delgado proximal Colostral antibodies absorption in dairy calves: I. Proximal small intestine study

    Directory of Open Access Journals (Sweden)

    Rosana Bessi

    2002-11-01

    Full Text Available Com o objetivo de estudar a morfologia e determinar a localização da enzima fosfatase ácida na região anterior do intestino delgado, do nascimento ao fechamento intestinal, foram coletadas amostras de 15 bezerros machos em três idades: ao nascer sem que houvesse a ingestão de colostro; três horas após a ingestão da primeira refeição de colostro e aos três dias de idade. Observou-se a presença de células vacuoladas do duodeno ao jejuno médio no recém-nascido, preenchidas por material absorvido após a ingestão de colostro. Foram verificadas mudanças nas características morfológicas aos três dias de idade, com o início da detecção de reação da fosfatase ácida em lisossomos, indicando ação enzimática sobre o material absorvido. A morfologia aos três dias de idade pode representar o diferente estádio de maturação das células epiteliais do intestino delgado de bezerros, indicando que o processo depende das características da primeira geração de células desta região do intestino.The objective of this study was to study the morphology and the localization of acid phosphatase at calves anterior small intestine, from birth to intestinal closure. Fifteen male dairy calves were used in this study, which were aged: unsuckled neonatal, three hours after colostrum ingestion and three days old. Vacuolated cells from duodenum to medium jejunum could be found in the newborn calf, which have shown absorbed material after colostrum ingestion. Changes at the morphological characteristics and the initiation of phosphatase acid reaction in lysosomes were observed in calves aged three days old. The three days old morphology can represent a different phase of epithelium cells maturation of calves small intestine indicating that the absorption process is dependent of the first generation of cells from this intestinal region.

  19. Comunicação não-verbal de pessoas portadoras de ostomias por câncer de intestino

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    Silene Oliveira Paegle

    2003-04-01

    Full Text Available Este estudo analisa a comunicação não-verbal de pessoas portadoras de ostomia, por câncer de intestino, integrantes de um grupo. É uma investigação de tipo exploratório, observacional, realizada com 5 pessoas portadoras de ostomias num hospital escola, durante 5 encontros de outubro a novembro de 2001. Os dados foram coletados através de filmagem e registro de diário de campo. Dentre os achados, destacamos que a sala onde ocorreram os encontros era pequena, obrigando os participantes a manterem uma distância íntima, o que provocou distorções na expressão espontânea das pessoas. O Estudo esclarece que a leitura da comunicação não-verbal possibilitou compreender o universo dos sujeitos pesquisados

  20. Linfoma Difuso de Grandes Células  de Intestino Delgado: Relato de Caso Diffuse Large -Cell Lymphoma of Small Bowel: Case Report

    Directory of Open Access Journals (Sweden)

    Gustavo Nunes Medina Coeli

    2012-06-01

    Full Text Available Introdução: Os tumores do intestino delgado são raros, de diagnóstico difícil e quando malignos, têm prognóstico ruim. Os linfomas primários representam menos de 2% de todos os tumores gastrointestinais malignos, sendo na sua maioria do subtipo de células B. Casuística: Foi relatado um caso raro de linfoma de intestino delgado em uma paciente do sexo feminino de 77 anos, que procurou atendimento médico com sintomas inespecíficos e um quadro anêmico. Os exames radiológicos foram fundamentais para esclarecimento, diagnóstico e propedêutica. Os marcadores tumorais foram negativos e as provas de atividade inflamatória, positivas. Na internação, a paciente teve piora súbita do quadro clínico, necessitando de cirurgia. No intra-operatório, foi identificado lesão perfurativa em jejuno proximal de aspecto ulcerado com aderências na bexiga e fundo do útero. A paciente não evoluiu bem, falecendo após três dias. A patologia confirmou Linfoma Não Hodgkin Difuso de Grandes Células B, com elevado índice de proliferação celular. Discussão: O diagnóstico radiológico pré-operatório do tumor do intestino delgado só é obtido em um pequeno percentual de pacientes sintomáticos. Estudos por imagem demonstram aspectos morfológicos do tipo infiltrativo, polipóide ou aneurismático. Geralmente, ocorre acometimento circunferencial da alça, com espessamento irregular das pregas, de extensão variável. Conclusão: O objetivo deste estudo foi documentar um raro tumor do intestino delgado do tipo Linfoma Não-Hodgkin Difuso de Grandes Células B, multicêntrico, de difícil diagnóstico e com rápida evolução dos sintomas, que culminaram com quadro de obstrução intestinal aguda, necessitando de cirurgia de emergência. Introduction: Tumors of the small bowel are rare, but when they are malignant they have a poor prognosis. The primary lymphomas represent less than 2% of all malignant gastrointestinal tumors, and the majority

  1. Efeito da dieta com restrição de lactose em pacientes com síndrome do intestino irritável

    OpenAIRE

    Marília Pinheiro César

    2013-01-01

    Introdução: A má digestão de lactose detectada por testes respiratórios é bastante comum em pacientes com síndrome do intestino irritável (SII), e alguns pacientes com SII referem exacerbar seus sintomas após a ingestão de leite e produtos lácteos. A fim de uma melhor percepção sobre este tema, este trabalho investigou os efeitos da dieta isenta de lactose em pacientes com SII e teste respiratório de hidrogênio para avaliação da digestão de lactose normal ou anormal. Objetivo: Avaliar os efei...

  2. Lichenoid reaction to carbamazepine in the oral mucosa: case report.

    Science.gov (United States)

    Artico, Gabriela; Bruno, Ingrid S; Seo, Juliana; Hirota, Silvio K; Acay, Renata; Migliari, Dante A

    2011-01-01

    Lichenoid drug reactions are more common in skin, but they may also occur in the oral mucosa. It is difficult to diagnose these lesions due to their clinical similarity to the idiopathic oral lichen planus lesions. The present article reports a case of lichenoid reaction in oral mucosa associated to the use of carbamazepine, emphasizing the diagnostic process.

  3. Efeitos do clampeamento do pedículo hepático nos intestinos Effects of the clamping of the hepatic pedículo in the intestines

    Directory of Open Access Journals (Sweden)

    Abrão Antonio Sébe

    2000-03-01

    Full Text Available Foi realizado clampeamento do pedículo hepático de rato por diferentes tempos e estudadas as repercussões nos intestinos. Para tanto foram utilizados 40 ratos, machos, divididos em quatro grupos de 10 animais cada. O grupo S (Sham não foi submetido a isquemia, já os grupos E1, E2 e E3 sofreram isquemia de 10, 20 e 30 minutos respectivamente. Nossos resultados mostraram alterações macroscópicas quanto a cor dos intestinos nos grupos E2 (20' e E3 (30' e histopatológicas que ocorreram em cada um desses grupos. Observou-se que quanto maior o tempo de clampeamento do pedículo hepático, maior a congestão esplâncnica, sendo que aos 30 minutos ocorreu o despreendimento do revestimento epitelial das vilosidades intestinais e hemorragia.It was made a clamping of the hepatic pedicle in different lapses of time and the morphological features of this intestines were observed. 40 male rats were divided in four groups of 10 animals each. The group S (Sham was not submitted to ischemia and the groups E1, E2 and E3 suffered ischemia during 10, 20 and 30 minutes respectively. Our results showed macroscopic modifications related to the colour of the intestines in the groups E2 (20' and E3 (30' as well as histopathological changes in the same groups. It was also observed that the larger the lapse of time of clamping of the hepatic pedicle the larger was the splanchnic congestion and in the group 30' detachment of the lining epithelium was observed in the intestinal villi.

  4. Effects Aerosol of Industrial Bleach and Detergent Mixture on Mucosa Layer and Lamina Mucosa Conjunctiva in Mice

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    Gh. Vaezi

    2013-06-01

    Full Text Available Today bleach and detergents are being frequently used and some people use their mixture for more cleaning. Because of chemical interaction of bleach and detergent, chlorine gas was released and thereby it could be dangerous for human health. This study examined the effects of exposed toxic mixture of bleach and detergent on the Mucosa layer and Lamina mucosa conjunctiva in the mice. In this study, 42 adult male mice NMRI race weighing 35-40 gr and from age 8 to 10 weeks were divided into 6 experimental groups and one control group. Experimental groups 1-2-3 with the use of chamber, the exposed 20 minutes were exposed to spray the amount 1 cc of mixture of bleach and detergent by nebulizer. Experimental groups 4-5-6 were for 35 minutes to inhale the same amount of material. Mice killed at 24-48-72 hours after exposed and the Mucosa Layer and Lamina mucosa conjunctiva tissue was studied pathology. In the study of microscopic sections prepared of mouse mucosa layer and Lamina mucosa conjunctiva tissue experimental group comparison with the control group, significant decrease was observed in mucosa layer the have (p ≤ 0.001  and significant decrease was observed in the Lamina mucosa have(p ≤ 0. 01,  p ≤ 0.001. As a result, increasing the exposed time of mixing bleach and detergent, as time passed, increasing the tissue damage and changes.

  5. Emprego da submucosa de intestino delgado porcina no reparo de lesões do esôfago cervical. Estudo experimental em cães Small intestine submucosa for repair of cervical esophageal lesions in a dog model

    Directory of Open Access Journals (Sweden)

    Zacarias Alves de Souza Filho

    2003-06-01

    Full Text Available OBJETIVO: Pesquisar a eficácia da submucosa de intestino delgado (SID porcina como enxerto para reparo de lesões em esôfago cervical de cães. MÉTODOS: Foram operados 10 cães. Ressecou-se parcialmente uma porção da parede anterior do esôfago que foi substituída por um enxerto de SID. Avaliaram-se a resistência tensiométrica, o aspecto macroscópico, a regeneração tecidual e a concentração de colágeno da porção esofágica onde se implantou o enxerto. RESULTADOS: Não houve infecção, fístula ou estenose. Verificou-se ganho semelhante de resistência do esôfago operado em relação ao controle: 31.84N contra 28.60N em média (p=0,593. A macroscopia revelou cicatrização completa com pouca retração tecidual. O estudo anátomo-patológico por HE mostrou re-epitelização completa da mucosa, proliferação vascular discreta a moderada e proliferação fibroblástica intensa. Na análise do colágeno pelo Sirus-red obteve-se em média 54.04% de colágeno tipo I, 16,04% de colágeno tipo III e 71.58% de colágeno total. CONCLUSÃO: A SID mostrou ser, no cão, um enxerto eficaz no reparo de lesões maiores do esôfago, apresentando-se resistente à infecção e à rejeição. A SID deve ser, portanto, considerada opção importante no tratamento destas lesões.PURPOSE: Study of porcine small intestinal submucosa (SIS efficiency as a graft in the handling of lesions of the cervical esophagus in dogs. METHODS: Ten dogs were operated. A resection of a portion of the anterior wall of the esophagus was performed, followed by its repair with a SIS graft. The tensiometric resistance, macroscopic appearance regeneration process and collagen contents of the esophagus tissue were evaluated. RESULTS: No cases of fistula, stenosis or infection occured. Tensiometric evaluation showed a similar resistance in the operated esophagus when compared to the control (an average of 31.84N against 28.60N. Macroscopic analysis revealed complete healing

  6. Miltefosina versus antimoniato de meglumina en el tratamiento de la leishmaniasis mucosa

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    María F García Bustos

    2014-10-01

    Full Text Available El tratamiento convencional para la leishmaniasis tegumentaria es el antimoniato de meglumina, el cual presenta falla terapéutica creciente, producción de efectos adversos graves, y necesidad de administración parenteral, justificando la búsqueda de alternativas terapéuticas. Presentamos aquí los resultados preliminares de un ensayo clínico de fase II en pacientes con leishmaniasis mucosa, en el que se comparó la eficacia de miltefosina por vía oral con respecto a la del compuesto antimonial. La evaluación de la respuesta a los tratamientos se realizó mediante un seguimiento con videofibroscopia nasofaríngea, utilizándose un score de gravedad de lesiones mucosas para aplicar en cada momento del seguimiento de los pacientes. No se encontraron hasta ahora diferencias significativas entre el número de pacientes curados con miltefosina o con la quimioterapia convencional. Los resultados favorables de este trabajo sugieren que miltefosina podría constituir una alternativa terapéutica efectiva y segura en la región.

  7. The oral mucosa in leprosy: a clinical and histopathological study.

    Science.gov (United States)

    de Abreu, Marilda Aparecida Milanez Morgado; Michalany, Nilceo Schwery; Weckx, Luc Louis Maurice; Neto Pimentel, Dalva Regina; Hirata, Cleonice Hitomi Watashi; de Avelar Alchorne, Maurício Mota

    2006-01-01

    Multibacillary leprosy may involve the oral mucosa, with or without apparent lesions. There are few studies that deal with this issue in the era of multidrug therapy. To assess the frequency of oral mucosa involvement in multibacillary leprosy patients. A transversal study with twenty non-treated multibacillary leprosy patients. The patients were treated in Dracena, São Paulo, between 2000 and 2002. Clinical examination of the oral mucosa was carried out. All patients were submitted to jugal mucosa, soft palate and tongue biopsies, in altered or in pre-established sites. The cross-sections were stained by techniques of hematoxilin-eosin and Ziehl-Neelsen. Granuloma and alcohol-acid-resistant bacilli findings determined the specific histopathological involvement. The study involved 19 patients with an average of 2.5 years of disease progression. Specific histopathological involvement occurred in the tongue and soft palate of one lepromatous patient with an apparently normal oral mucosa. (1) Clinical alterations in the oral mucosa does not imply disease involvement, it is necessary to have histopathological confirmation. (2) Apparent specific clinical alterations are rare. (3) The clinically normal oral mucosa can show specific histopathological involvement.

  8. Perfil de citocinas proinflamatorias y antiinflamatorias en pacientes pediátricos con síndrome de intestino irritable

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    R. Vázquez-Frias

    2015-01-01

    Conclusiones: Este estudio indica que los niños con SII presentan un estado de alteración de la regulación inmune. Aún está pendiente por dilucidar el papel que juegan estas citocinas, específicamente la TGF-β, en la patogénesis del SII.

  9. Microstructure imaging of human rectal mucosa using multiphoton microscopy

    Science.gov (United States)

    Liu, N. R.; Chen, G.; Chen, J. X.; Yan, J.; Zhuo, S. M.; Zheng, L. Q.; Jiang, X. S.

    2011-01-01

    Multiphoton microscopy (MPM) has high resolution and sensitivity. In this study, MPM was used to image microstructure of human rectal mucosa. The morphology and distribution of the main components in mucosa layer, absorptive cells and goblet cells in the epithelium, abundant intestinal glands in the lamina propria and smooth muscle fibers in the muscularis mucosa were clearly monitored. The variations of these components were tightly relevant to the pathology in gastrointestine system, especially early rectal cancer. The obtained images will be helpful for the diagnosis of early colorectal cancer.

  10. Dorsal onlay graft bulbar urethroplasty using buccal mucosa

    African Journals Online (AJOL)

    G. Barbagli

    2015-12-02

    Dec 2, 2015 ... promote the transformation of the urethral mucosa plate into a tube, according to ... Allen stirrups and sequential inflatable compression sleeves. Figure 2 .... the ventral, dorsal or lateral surface of the urethra, we investigated if.

  11. Reconstruction of highly contracted socket after irradiation with antral mucosa

    International Nuclear Information System (INIS)

    Tanabe, Yosihiko; Masaki, Michiyosi; Kato, Hisakazu

    1999-01-01

    We have repaired 3 cases of the highly contracted socket after irradiation by lining it using antral mucosa and obtained excellent results. Although this procedure requires rhinological skill to obtain the mucosa, it has an advantage leaving no visible scar at the donor site. Usually it is not difficult to obtain a sufficient quantity of the mucous membrane to line a whole socket from one antrum. Besides, it is also easy to line its surface since the shape of antral mucosa is originally in a sac form. All we have to do is making 20 mm long incision to the mucosa, putting a silicone conformer into it, and inlaying it into the graft bed. Thus, having once obtained the mucous membrane, the surgical procedure itself is a quite simple one. (author)

  12. Serotonin Immunoreactive Cells and Nerve Fibers in the Mucosa of ...

    African Journals Online (AJOL)

    hydroxytryptamine) immunoreactivity in the pyloric mucosa of the rat stomach. The immunoreactive elements included the endocrine cells, mast cells and mucosal nerve fibers in the lamina propria. The immunopositive endocrine cells were oval in ...

  13. Roseomonas mucosa Isolated from Bloodstream of Pediatric Patient ▿

    Science.gov (United States)

    Bard, J. Dien; Deville, J. G.; Summanen, P. H.; Lewinski, M. A.

    2010-01-01

    We report a case of catheter-related bacteremia associated with Roseomonas mucosa isolated from an immunocompromised pediatric patient with a history of multiple episodes of urinary tract infection and bacteremia. PMID:20534804

  14. Habitual biting of oral mucosa: A conservative treatment approach

    Directory of Open Access Journals (Sweden)

    Sarabjot Kaur Bhatia

    2013-01-01

    Full Text Available Chronic biting of oral mucosa is an innocuous self inflicted injury, commonly seen in children suffering from developmental and psychological problems and has rarely been reported in normal unaffected individuals. The management strategies vary from counseling, prescription of sedatives to different prosthetic shields. The paper highlights the efficacy of a simple approach using soft mouth guard in the management of self inflicted lesions due to habitual biting of oral mucosa in two normal healthy children.

  15. Studies on nerve terminations in human mucosa and skin

    OpenAIRE

    Hilliges, Marita

    1997-01-01

    - In spite of their accessibility and important sensory function,the nervous tissue components of human oral and vaginal mucosa and skin have beensubject to very few, if any, systematic investigations. Studies on the innervationof oral tissues have mainly focused on the dental pulp, the periodontium and thegingiva, probably because of specific clinical interest, thus largely neglectingthe mucosa. Genital studies comprise only in a few cases the vagina and when thevagina is i...

  16. Effects of radiation on nasal mucosa of guinea pig

    International Nuclear Information System (INIS)

    Xiao Mang; Hu Sunhong; Liu Yuehui

    2004-01-01

    Objective: To study histopathological changes in nasal mucosa of guinea pigs following a series of irradiation. Methods: A total of 60 healthy guinea pigs were divided into two groups randomly: the irradiation group (n=30) and the control group (n=30). The animals of the irradiation group were exposed nasally to linear accelerator X-rays at 5.0 Gy once a week for three weeks. At 1 d, 1 w, 2 w, 4 w, 10 w and half year after X-irradiation five animals per one group were sacrificed randomly, at each time point. Their middle nasal turbinate mucosa was observed under optical microscope and electron microscope, and were analysed by image analysis for histopathological transformation. Results: In the irradiation group, the early histopathological transformation was acute inflammatory reaction, at the fourth week the mucosa began to repair, which ended at the sixth month. But after repaired, some parts of the repaired mucosa were lined with squamous epithelium instead of the normal nasal mucosa. The rate of the cilia-covered area was only 52.9% at the sixth month. Conclusion: The nasal mucosa injury and the squamous metaplasia could form a pathological basis of dysfunction after irradiation

  17. Tuberculoma del mesenterio de gran tamaño: Extirpación, resección de sesenta centímetros de intestino

    Directory of Open Access Journals (Sweden)

    Manuel José Luque

    1944-04-01

    Full Text Available Tuvo viruela hace varios años y fufrió de frecuentes grippes. Menarquia a los 16 años, hace cinco meses tuvo un retardo menstrual que le duro ocho semanas, mas tarde reapareció el periodo sin dolores ni perturbaciones de ninguna clase. En la actualidad esta nuevamente en amenorrea, que lleva mes y medio. Es madre de ocho hijos todos sanos y normales y no ha tenido abortos.

  18. Tricholeiperia peruensis n. sp. (Nematoda, Molineidae) del quiróptero Lophostoma silvicolum occidentalis (Phyllostomidae) en Tumbes, Perú

    OpenAIRE

    Vargas, Marina; Martínez, Rosa; Tantaleán, Manuel; Cadenillas, Richard; Pacheco, Víctor

    2008-01-01

    En el presente trabajo, se describe a Tricholeiperia peruensis n. sp. (Nematoda, Molineidae) en base a 25 especímenes colectados del intestino de 5 murciélagos de la especie Lophostoma silvicolum occidentalis de la localidad de Angostura, distrito Pampas de Hospital, departamento de Tumbes, Perú. La nueva especie se caracteriza por la morfología de la vesícula cefálica en forma de capuchón con escotaduras, por el tamaño y forma de las espículas cuyo extremo distal de la rama externa se divide...

  19. Teste do H2 no ar expirado na avaliação de absorção de lactose e sobrecrescimento bacteriano no intestino delgado de escolares

    Directory of Open Access Journals (Sweden)

    REIS Jairo César dos

    1999-01-01

    Full Text Available Estudos realizados há mais de 10 anos demonstraram que a deficiência ontogenética de lactase é freqüente na população brasileira. Entretanto, esses estudos se basearam no incremento da glicemia após sobrecarga de doses não utilizadas habitualmente na dieta. Atualmente, aceita-se que a avaliação da absorção da lactose com o teste do hidrogênio no ar expirado é mais apropriada que o teste da curva glicêmica. Por outro lado, a enteropatia ambiental sintomática e/ou assintomática constituem um grave problema de saúde pública em grupos populacionais de baixo nível sócio-econômico de nosso meio. A ocorrência de sobrecrescimento bacteriano no intestino delgado de crianças com enteropatia ambiental, foi caracterizada em crianças moradoras em favelas. Sobrecrescimento bacteriano no intestino delgado é caracterizado pela presença de bactérias pertencentes à flora colônica no intestino delgado. Objetivando avaliar a freqüência de má absorção de lactose e determinar a ocorrência de sobrecrescimento bacteriano no intestino delgado em crianças escolares, através do teste do H2 no ar expirado, investigaram-se 83 alunos de uma escola localizada na região periférica da cidade de Marília, Estado de São Paulo, após sobrecarga oral de lactose e lactulose. Foram colhidas também amostras de fezes para pesquisa de parasitas. Má absorção de lactose foi observada em 19 (22,9% escolares e intolerância à lactose em 10 (12,0%. Sobrecrescimento bacteriano no intestino delgado foi identificado pelo teste do H2 no ar expirado em 6 (7,2% dos 83 escolares. Giardia lamblia foi o parasita mais freqüentemente isolado nas fezes dos escolares. A ocorrência de deficiência ontogenética de lactase observada neste grupo de crianças foi semelhante ao relatado para populações caucasianas. Sobrecrescimento bacteriano foi encontrado neste grupo de crianças assintomáticas, podendo refletir as condições desfavoráveis de seu meio

  20. BIOMETRIC STUDY OF COMMERCIAL ALAYING HENS SUBMITTED TO THE PROGRAMS OF FORCED MOLTING BIOMETRIA DO INTESTINO DE POEDEIRAS COMERCIAIS SUBMETIDAS A PROGRAMAS DE MUDA FORÇADA

    Directory of Open Access Journals (Sweden)

    Lizandra Amoroso

    2008-12-01

    Full Text Available The forced molting in commercial laying hens had being utilized for get better the performance of birds for one more cycle of production of eggs. The experiment was aimed at evaluating the intestinal biometric parameters in laying hens submitted to the different forced molting program. Third two Hisex Brown laying hen with 58 weeks of age were  randomly subdivided into four programs (P1- California program; P2- diet of low-calcium; T3- diet of high-zinc and P4- diet of low-sodium in two replications constituted of four birds each. The biometric parameters occurred at 28th and 140nd days and for this, four birds were sacrificed of each program in the period cited. The bird corporal weight and the relative weight of the intestine (duodenum, jejunum, ileum, cecum and rectum were obtained on electronic precision scales and the lengths were measured. The data were submitted to the variance analysis and in case of significant difference; the averages were compared by the Tukey´s test. It was observed the commercial laying P1 and P3 the smallest mean body and relative weights (no significant and lengths in relation to the chickens P2 and P4 and to the 140th days the corporal weight, relative weight and length of the intestine of chickens were larger. Concluded that the different programs of forced molting presented similar results and they can be used for commercial laying hens in second production cycle.

    KEY WORDS: Digestory system, nutricional manage, physiology, postmolt.
    A muda forçada em poedeiras comerciais tem sido utilizada para melhorar o desempenho zootécnico das aves por mais um ciclo de produção de ovos. O experimento foi realizado com o objetivo de avaliar a biometria do intestino de poedeiras comerciais submetidas a diferentes programas de muda forçada. Trinta e duas aves da linhagem Hisex Brown com 58 semanas de idade foram distribuídas em um delineamento inteiramente casualizado, constando de quatro programas de

  1. Frecuencia de las enfermedades de la mucosa bucal en el Uruguay en niños de 0 a 14 años

    Directory of Open Access Journals (Sweden)

    Verónica Keochgerián

    Full Text Available Se realizó un estudio clínico-epidemiológico, analítico y transversal en 401 individuos de 0 a 14 años, distribuidos en dos muestras aleatorias, provenientes del Instituto Nacional del Niño y Adolescente del Uruguay (INAU y el Centro de Asistencia del Sindicato Médico del Uruguay (CASMU. El objetivo fue establecer la prevalencia de las enfermedades de la mucosa bucal en 401 pacientes y en cada una de las muestras; determinar la influencia del género, la edad, la localización en la mucosa, la situación socio-económica-cultural, establecer la frecuencia relativa por categorías de enfermedades y comparar los resultados obtenidos con estudios similares de otrosmedios. La prevalencia total fue de 32.9%; en CASMU 36.8% y en INAU 29%. Las variables edad y género no determinaron diferencias significativas. Se consideraron lesiones: traumáticas, de desarrollo, infecciosas, inmunoalérgicas, crecimientos tisulares, por acción del tabaco e idiopáticas. Las lesiones más prevalentes fueron las traumáticas y las de desarrollo

  2. Leptin promotes wound healing in the oral mucosa.

    Science.gov (United States)

    Umeki, Hirochika; Tokuyama, Reiko; Ide, Shinji; Okubo, Mitsuru; Tadokoro, Susumu; Tezuka, Mitsuki; Tatehara, Seiko; Satomura, Kazuhito

    2014-01-01

    Leptin, a 16 kDa circulating anti-obesity hormone, exhibits many physiological properties. Recently, leptin was isolated from saliva; however, its function in the oral cavity is still unclear. In this study, we investigated the physiological role of leptin in the oral cavity by focusing on its effect on wound healing in the oral mucosa. Immunohistochemical analysis was used to examine the expression of the leptin receptor (Ob-R) in human/rabbit oral mucosa. To investigate the effect of leptin on wound healing in the oral mucosa, chemical wounds were created in rabbit oral mucosa, and leptin was topically administered to the wound. The process of wound repair was histologically observed and quantitatively analyzed by measuring the area of ulceration and the duration required for complete healing. The effect of leptin on the proliferation, differentiation and migration of human oral mucosal epithelial cells (RT7 cells) was investigated using crystal violet staining, reverse transcription polymerase chain reaction (RT-PCR) and a wound healing assay, respectively. Ob-R was expressed in spinous/granular cells in the epithelial tissue and vascular endothelial cells in the subepithelial connective tissue of the oral mucosa. Topical administration of leptin significantly promoted wound healing and shortened the duration required for complete healing. Histological analysis of gingival tissue beneath the ulceration showed a denser distribution of blood vessels in the leptin-treated group. Although the proliferation and differentiation of RT7 cells were not affected by leptin, the migration of these cells was accelerated in the presence of leptin. Topically administered leptin was shown to promote wound healing in the oral mucosa by accelerating epithelial cell migration and enhancing angiogenesis around the wounded area. These results strongly suggest that topical administration of leptin may be useful as a treatment to promote wound healing in the oral mucosa.

  3. A study of complexity of oral mucosa using fractal geometry

    Directory of Open Access Journals (Sweden)

    S R Shenoi

    2017-01-01

    Full Text Available Background: The oral mucosa lining the oral cavity is composed of epithelium supported by connective tissue. The shape of the epithelial-connective tissue interface has traditionally been used to describe physiological and pathological changes in the oral mucosa. Aim: The aim is to evaluate the morphometric complexity in normal, dysplastic, well-differentiated, and moderately differentiated squamous cell carcinoma (SCC of the oral mucosa using fractal geometry. Materials and Methods: A total of 80 periodic acid–Schiff stained histological images of four groups: normal mucosa, dysplasia, well-differentiated SCC, and moderately differentiated SCC were verified by the gold standard. These images were then subjected to fractal analysis. Statistical Analysis: ANOVA and post hoc test: Bonferroni was applied. Results: Fractal dimension (FD increases as the complexity increases from normal to dysplasia and then to SCC. Normal buccal mucosa was found to be significantly different from dysplasia and the two grades of SCC (P < 0.05. ANOVA of fractal scores of four morphometrically different groups of buccal mucosa was significantly different with F (3,76 = 23.720 and P< 0.01. However, FD of dysplasia was not significantly different from well-differentiated and moderately differentiated SCC (P = 1.000 and P = 0.382, respectively. Conclusion: This study establishes FD as a newer tool in differentiating normal tissue from dysplastic and neoplastic tissue. Fractal geometry is useful in the study of both physiological and pathological changes in the oral mucosa. A new grading system based on FD may emerge as an adjuvant aid in cancer diagnosis.

  4. Caracterización de los efectos de la administración de la lectina antinutricional nigrina de corteza de Sambucus nigra L. y efecto sinérgico con los polifenoles del té verde

    OpenAIRE

    Cabrero Lobato, Patricia

    2012-01-01

    Las proteínas inactivadoras de ribosomas (RIPs) son enzimas con actividad N-glicosidasa del ARN ribosómico. Algunas poseen dos cadenas una con actividad enzimática y otra con actividad lectina (RIPs de tipo dos). Entre estas RIPs se encuentra la nigrina b presente en la corteza de Sambucus nigra L. La nigrina b presenta toxicidad selectiva en función de la dosis y la vía de administración. El blanco son las células de las criptas del intestino delgado. Nuestra hipótesis es que los efectos tóx...

  5. The effects of glutamine-supplemented diet on the intestinal mucosa of the malnourished growing rat Os efeitos de dieta com suplementação de glutamina sobre a mucosa intestinal do rato desnutrido em crescimento

    Directory of Open Access Journals (Sweden)

    Uenis Tannuri

    2000-06-01

    types of chow-based diet. The glutamine-enriched diet resulted in the greatest reduction of crypt depth, and this reduction was also statistically significant when compared with control animals. CONCLUSIONS: Enteral glutamine has some positive effects on body weight gain and trophism of the jejunal mucosa in the malnourished growing rat.A glutamina é o aminoácido mais abundante no sangue e exerce papel importante na resposta do intestino delgado às agressões sistêmicas. Atrofia da mucosa intestinal ocorre em algumas afecções clínicas como desnutrição grave. Foi demonstrado que a glutamina tem ação trófica em situações como período p��s-operatório de ressecção ou transplante intestinal, radioterapia, trauma cirúrgico, isquemia intestinal ou administração de drogas citotóxicas. Tais estudos não foram realizados em animais desnutridos em fase de crescimento. Desta forma, no presente trabalho verificamos se a glutamina exerce ação trófica sobre a mucosa intestinal do rato desnutrido em fase de crescimento. Foram utilizadas 35 ratas com 21 dias de idade, e divididas em 4 grupos: controle - dieta normal; desnutrição - provocada por diarréia induzida pela administração de dieta rica em lactose durante 15 dias; desnutrição+dieta normal durante os 15 dias subseqüentes; desnutrição + dieta rica em glutamina (2%. Após 30 dias de experimento os animais foram pesados, mortos e um segmento de jejuno foi colhido para estudos histológicos e histomorfométricos. Os grupos de animais apresentaram médias de pesos semelhantes no primeiro dia de estudo, sendo que a alimentação com dieta rica em lactose (grupo desnutrido provocou significativa queda de peso em relação aos controles. A re-alimentação com ambos os tipos de dieta promoveu ganho significativo de peso corpóreo, embora a dieta rica em glutamina tenha sido mais eficaz. Os estudos histológicos e histomorfométricos demonstraram que a desnutrição provocou significativa redu

  6. [Oral mucosa reaction in patients adapting to removable dentures].

    Science.gov (United States)

    Iordanishvili, A K; Soldatova, L N; Pihur, O L; Mihajlova, E S; Peremyshlenko, A S; Soldatov, V S

    Oral mucosa reaction of prosthetic bed to the removable acrylic dentures was evaluated in 43 patients (12 male and 31 female) aged 56-69 years with partial and full teeth loss in one or both jaws. Patients of the first (control) group (17 patients) were not using additional tools improving fixation of the removable dentures during adaptation period, while patients of the second (main) group (26 patients) used Corega cream for dentures fixation for 30 days follow-up. Oral mucosa assessment was carried out on 3-4 and 28-30 day of dentures use by 3 end points: pain syndrome, moisture level, inflammation of a prosthetic bed. The results proved Corega cream to improve prosthetic bed mucosa condition reducing inflammatory response to polymeric materials of removable dentures basis.

  7. Speech and swallowing outcomes in buccal mucosa carcinoma

    Directory of Open Access Journals (Sweden)

    Sunila John

    2011-01-01

    Full Text Available Buccal carcinoma is one of the most common malignant neoplasms among all oral cancers in India. Understanding the role of speech language pathologists (SLPs in the domains of evaluation and management strategies of this condition is limited, especially in the Indian context. This is a case report of a young adult with recurrent squamous cell carcinoma of the buccal mucosa with no deleterious habits usually associated with buccal mucosa carcinoma. Following composite resection, pectoralis major myocutaneous flap reconstruction, he developed severe oral dysphagia and demonstrated unintelligible speech. This case report focuses on the issues of swallowing and speech deficits in buccal mucosa carcinoma that need to be addressed by SLPs, and the outcomes of speech and swallowing rehabilitation and prognostic issues.

  8. Harvesting oral mucosa for one-stage anterior urethroplasty

    Directory of Open Access Journals (Sweden)

    Sanjay Balwant Kulkarni

    2014-01-01

    Full Text Available Oral mucosa has been the most popular substitute material for urethral reconstructive surgery because it is easy to harvest, is easy to access, has a concealed donor site scar, and obviates most of the problems associated with other grafts. However, the success of using oral mucosa for urethral surgery is mainly attributed to the biological properties of this tissue. Herein, the surgical steps of harvesting oral mucosa from the inner cheek are presented with an emphasis on tips and tricks to render the process easier and more reproducible and to prevent intra and post-operative complications. The following steps are emphasized: Nasal intubation, ovoid shape graft, delicate harvesting leaving the muscle intact, donor site closure and removal of submucosal tissue.

  9. Characterizing lamina propria of human gastric mucosa by multiphoton microscopy

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Y C; Yang, H Q; Zhuo, S M [Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou 350007 (China); Chen, G; Chen, J X [Department of Pathology, Fujian Provincial Tumor Hospital, Fuzhou, 350014 (China); Yan, J, E-mail: chenjianxin@fjnu.edu.cn, E-mail: ynjun@yahoo.com [Department of Surgery, Fujian Provincial Tumor Hospital, Fuzhou, 350014 (China)

    2011-01-01

    Lamina propria (LP) of gastric mucosa plays an important role in progression of gastric cancer because of the site at where inflammatory reactions occur. Multiphoton imaging has been recently employed for microscopic examination of intact tissue. In this paper, using multiphoton microscopy (MPM) based on two-photon excited fluorescence (TPEF) and second harmonic generation (SHG), high resolution multiphoton microscopic images of lamina propria (LP) are obtained in normal human gastric mucosa at excitation wavelength {lambda}{sub ex} = 800 nm. The main source of tissue TPEF originated from the cells of gastric glands, and loose connective tissue, collagen, produced SHG signals. Our results demonstrated that MPM can be effective for characterizing the microstructure of LP in human gastric mucosa. The findings will be helpful for diagnosing and staging early gastric cancer in the clinics.

  10. Characterizing lamina propria of human gastric mucosa by multiphoton microscopy

    Science.gov (United States)

    Liu, Y. C.; Yang, H. Q.; Chen, G.; Zhuo, S. M.; Chen, J. X.; Yan, J.

    2011-01-01

    Lamina propria (LP) of gastric mucosa plays an important role in progression of gastric cancer because of the site at where inflammatory reactions occur. Multiphoton imaging has been recently employed for microscopic examination of intact tissue. In this paper, using multiphoton microscopy (MPM) based on two-photon excited fluorescence (TPEF) and second harmonic generation (SHG), high resolution multiphoton microscopic images of lamina propria (LP) are obtained in normal human gastric mucosa at excitation wavelength λex = 800 nm. The main source of tissue TPEF originated from the cells of gastric glands, and loose connective tissue, collagen, produced SHG signals. Our results demonstrated that MPM can be effective for characterizing the microstructure of LP in human gastric mucosa. The findings will be helpful for diagnosing and staging early gastric cancer in the clinics.

  11. [Oral mucosa graft urethroplasty for complicated urethral strictures].

    Science.gov (United States)

    Horiguchi, Akio; Sumitomo, Makoto; Kanbara, Taiki; Tsujita, Yujiro; Yoshii, Takahiko; Yoshii, Hidehiko; Satoh, Akinori; Asakuma, Junichi; Ito, Keiichi; Hayakawa, Masamichi; Asano, Tomohiko

    2010-03-01

    We evaluated the efficacy and outcome of one-stage oral mucosa graft urethroplasty, which is currently the procedure of choice for treating lengthy and complicated urethral strictures not amenable to excision and primary end-to-end anastomosis. Seven patients 33 to 74 years old (mean age = 53.7) underwent one-stage oral mucosa graft urethroplasty for a stricture in either the bulbar urethra (four patients), penile urethra (two patients), or pan-anterior urethra (one patient). Three of the strictures were due to trauma, one was due to inflammation, and one was due to a failed hypospadia repair. The other two were iatrogenic. All patients had previously undergone either internal urethrotomy or repeated urethral dilation. Three patients received a tube graft, three received a ventral onlay, and one received a dorsal onlay. A free graft of oral mucosa was harvested from the inside of each patient's left cheek, and if necessary to obtain a sufficient length, the harvest was extended to include mucosa from the lower lip and the right cheek. The graft lengths ranged from 2.5 to 12 cm (mean = 4.6 cm). A urethral catheter was left in place for 3 weeks postoperatively. While no severe complications at the donor site were observed during follow-up periods ranging from 3 to 55 months (mean = 14 months), two patients who had received a tube graft developed distal anastomotic ring strictures that were managed by internal urethrotomy. The other five required no postoperative urological procedure even though one who had received a ventral onlay developed a penoscrotal fistula. Oral mucosa is an ideal urethral graft, and oral mucosa graft urethroplasty is an effective procedure for repairing complicated urethral strictures involving long portions of the urethra.

  12. [Microcirculation of the nasal mucosa during use of balloon tamponade].

    Science.gov (United States)

    Klinger, M; Siegert, R

    1997-03-01

    Nasal packings are commonly accepted in the treatment of severe epistaxis. Cuffed catheters are known to cause damage to the nasal mucosa most likely by interfering with tissue perfusion. In this study the effect of different pressure levels on local perfusion of septal mucosa is investigated. In 15 healthy subjects the blood flow in septal mucosa was measured by laser doppler flowmetry by positioning a cuffed epistaxis catheter into the nasal cavity with a laser probe attached to it. Increasing pressure was administered by injecting saline solution while continuously recording intraluminal pressure, perfusion, and filling volume. The local pressure affecting the septal mucosa at the moment of stalling perfusion was determined by subtracting the extranasal cuff pressure from the current intranasal cuff pressure at same inflation volumes. Microcirculation of the septal mucosa stopped when the local pressure exceeded a value of Pmean = 42 mmHg. Individual variations (n = 15) were small (s = 9 mmHg). The intraluminal cuff pressure was measured to be about ten times higher due to the retraction force of the cuff. Spontaneous oscillations of the blood flow were reduced with increasing pressure to the blood vessels. Filling volumes up to 3.2 ml were sufficient to stop perfusion. Cuffed nasal packings stop the blood flow in nasal mucosa even at low local pressures. Depending on the material characteristics of different cuffs the pressure to dilate the cuff may, however, be several times higher than the actual local pressure. This effect may cause problems in the proper use of cuffed catheters. Laser doppler flowmetry proved to be helpful in determining reproducible perfusion values.

  13. Influência da suplementação de glutamina sobre o desempenho e o desenvolvimento de vilos e criptas do intestino delgado de frangos Influence of glutamine supplementation on performance and intestinal villous and crypt development in broiler chickens

    Directory of Open Access Journals (Sweden)

    A. Maiorka

    2000-10-01

    Full Text Available Investigou-se o efeito da suplementação de glutamina na dieta sobre o consumo de ração, ganho de peso e conversão alimentar e sobre a estrutura da mucosa intestinal de frangos. Foram utilizados 320 pintos de corte machos distribuídos em um delineamento inteiramente ao acaso com dois tratamentos e quatro repetições, sendo T1 suplementado com 1% de L-glutamina na dieta e T2 controle. Os índices de desempenho foram analisados aos 7, 21 e 49 dias de idade das aves. Aos 7 e 14 dias de idade oito aves foram sacrificadas para colheita de fragmentos de cada porção do intestino delgado para avaliação da morfometria intestinal em microscopia de luz em sistema analisador de imagens "Video Plan". As variáveis estudadas foram altura dos vilos, profundidade de cripta e relação vilo: cripta. A adição de 1% de glutamina à dieta de frangos não influenciou (P>0,05 o seu desempenho zootécnico. Entretanto, 1% de glutamina na ração foi capaz de alterar (PThis investigation was carried out in order to study the influence of glutamine supplementation in the ration on performance and on the intestinal structure development of broiler chickens. Three hundred and twenty day-old broiler chickens were used in a completely randomized experiment with two treatments and four repetitions, being T1 supplemented with 1% L-glutamine and T2 - control (not supplemented. The performance was evaluated (feed intake, body weight gain and feed conversion at 7, 21 and 49 days of age. At the same ages birds were sacrificed and samples from different parts of the intestine were collected (duodenum, jejunum and ileum to be submitted to morphometric studies under light microscopy using an image analysis system (Video Plan. The variables studied were villous height, crypt depth and villous:crypt ratio. It was demonstrated that 1% glutamine supplementation in the ration did not influence the broiler chicken performance (P>0.05 during the different phases of growth

  14. X-radiation effect on soluble proteins of gastric mucosa

    International Nuclear Information System (INIS)

    Sukhomlinov, B.F.; Chajka, Ya.P.; Fedorovich, A.N.

    1979-01-01

    Using the method of electrophoresis in agar gel soluble proteins of gastric mucosa of rats were separated into 11 fractions. Proteins posessing a proteolytic (pH 1.8) and lipase (pH 7.4) activity were localized within the second and third prealbumin fractions. Soluble proteins of gastric mucosa contain glyco- and lipoproteid complexes. Exposure of rats to 1000 R of X-rays induces quantitative redistribution within the electrophoretic spectrum of soluble proteins and a considerable disturbance of the proteolytic activity of total soluble proteins throughout the entire period of observation (from 10 min to 72h)

  15. Adherence of Helicobacter pylori to the Gastric Mucosa

    Directory of Open Access Journals (Sweden)

    Marguerite Clyne

    1997-01-01

    Full Text Available Bacterial adhesion to the intestinal epithelium is a critical initial step in the pathogenesis of many enteric diseases. Helicobacter pylori is a duodenal pathogen that adheres to the gastric epithelium and causes gastritis and peptic ulceration. The mechanism by which H pylori causes disease has not yet been elucidated but adherence to the gastric mucosa is thought to be an important virulence determinant of the organism. What is known about adherence of H pylori to the gastric mucosa is summarized. Topics discussed are the mechanism of H pylori adherence; in vitro and in vivo models of H pylori infection; and adherence and potential adhesins and receptors for H pylori.

  16. Estudio de la mucosa oral en pacientes que emplean colutorios

    OpenAIRE

    Marzal Gamarra, Cristina

    2009-01-01

    INTRODUCCIÓN 1. Recuerdo histológico de la mucosa oral La cavidad bucal, como toda cavidad orgánica que se comunica con el exterior, esta tapizada por una membrana mucosa de superficie húmeda. La humedad, que es aportada por las glándulas salivales mayores y menores, es necesaria para el mantenimiento de la estructura normal de los tejidos. 2. Colutorios y su utilización en Odontología Los colutorios son preparaciones líquidas destinadas a ser aplicadas sobre los dientes, las...

  17. Alphamimetic effects at the nasal mucosa in the MR tomogram

    International Nuclear Information System (INIS)

    Benammar-Englmaier, M.; Hallermeier, J.K.; Englmaier, B.

    1990-01-01

    The effect of xylometazolin spray (OTRIVEN) on the nasal mucosa was tested on persons with and without clinical signs of rhinitis and sinusitis. MRI is an excellent means to show the decongestive effect. The reaction is chiefly seen at the inferior and middle nasal concha and becomes manifest in a few minutes. No significant effect is noticeable at the maxillary sinus mucosa and infiltrated ethmoidal sinus. Generally, it is possible to show and examine the pharmacological effects that change the size and the liquid consistency of organs. (orig.) [de

  18. Absorção de anticorpos do colostro em bezerros: II. Estudo no intestino delgado distal Colostral antibodies absorption in calves: II. Distal small intestine

    Directory of Open Access Journals (Sweden)

    Rosana Bessi

    2002-11-01

    Full Text Available Com o objetivo de estudar a morfologia e determinar a localização da enzima fosfatase ácida na região distal do intestino delgado de bezerros, do nascimento ao fechamento intestinal, foram coletadas amostras de 15 animais machos em três idades: ao nascer sem que houvesse a ingestão de colostro; três horas após a ingestão da primeira refeição de colostro e aos três dias de idade. Observou-se, ao nascimento, a presença de um grande vacúolo, que dominava todo o citoplasma das células epiteliais do jejuno distal e íleo. Após a ingestão de colostro, verificou-se o acúmulo de material absorvido nesses vacúolos. Foi detectada a reação de fosfatase ácida nas células absortivas de bezerros recém-nascidos, antes e após a ingestão de colostro. Aos três dias de idade, uma nova população de células geralmente não vacuoladas, com sistema endocítico apical reduzido, foi observada recobrindo as vilosidades intestinais. Portanto, em bezerros a maturação do epitélio absortivo do intestino delgado distal pode iniciar-se com o aumento da atividade enzimática nos vacúolos absortivos, culminando com a rápida substituição das células fetais por células diferenciadas não pinocíticas, o que determinaria o término da transferência de anticorpos maternos.The localization of acid phosphatase at distal small intestine and its morphology were studied f0rom birth to intestinal closure from fifteen male dairy calves aged: unsuckled neonatal, three hours after colostrum ingestion and three days old. At birth, the presence of a large vacuole was found and it expanded all over the epithelial cells cytoplasm at distal jejunum and ileum. For colostrum fed calves, ingested material could be observed in the vacuole. The phosphatase acid reaction was detected in the absorptive cells of suckled and unsuckled newborn calves. Calves aged three days old, a new population of non-vacuolated cells and reduced apical endocytic system were found

  19. Alimentação como fator de risco para câncer de intestino em universitários

    Directory of Open Access Journals (Sweden)

    Liliane Almeida

    2017-03-01

    Full Text Available Objetivo: Analisar a qualidade da alimentação de universitários como fator de risco para câncer de intestino. Métodos: Estudo transversal com 100 universitários do curso de Gastronomia de uma instituição de ensino privada em Montes Claros, Minas Gerais. Aferiu-se o índice de massa corporal (IMC. Coletaram-se o comportamento alimentar, prática de atividade física e a frequência alimentar. Utilizou-se a análise descritiva. Resultados: A partir dos resultados, verificou-se que 46% (n=46 dos estudantes estavam com a massa corporal elevada, ou seja, IMC ≥ 25 kg/m2 (sobrepeso e obesidade. A análise dos hábitos alimentares demonstrou mudança de peso corporal em 44% dos entrevistados, destes 25% apresentou ganho de massa corporal. Dentre os entrevistados, 41% alegou consumir embutidos, pizzas e fast foods dentre outros, e 57% relatou consumir alimentos industrializados de 1 a 3 vezes por semana. Os alimentos ricos em açúcar (café com açúcar, refrigerantes e chocolate eram consumidos diariamente por 71%, 30% e 24% dos participantes. O consumo de grãos (chia, linhaça e quinoa foi relatado por 7%. Conclusão: Observou-se alto consumo de alimentos processados e/ou industrializados com alto teor de gorduras e açúcares, juntamente com uma baixa ingestão de grãos. Essa conjuntura alimentar associada a um estilo de vida sedentário são fatores de risco para a instalação de um quadro de neoplasia.

  20. Terapia nutricional oral em pacientes com Síndrome do Intestino Curto Oral dietetic therapy in patients with Short Bowel Syndrome

    Directory of Open Access Journals (Sweden)

    Carla Barbosa NONINO

    2001-12-01

    Full Text Available A Síndrome do Intestino Curto representa um dos quadros mais sérios e graves de má-absorção e a terapêutica dietética destes pacientes é um desafio importante a ser enfrentado. Este trabalho teve como objetivo descrever a terapia dietética via oral e a sua participação em evitar a desnutrição protéico-energética em portadores de Síndrome do Intestino Curto seguidos na Unidade Metabólica da Divisão de Nutrologia do Departamento de Clínica Médica da Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo. Foram acompanhados 12 pacientes, 7 homens e 5 mulheres, idade de 55 ± 10 anos, por um período de 24 meses. Eles receberam, concomitantemente à nutrição parenteral central ou periférica, dieta via oral fracionada em 7 a 8 refeições/dia, em intervalos regulares e constantes, com pequeno volume por refeição, hipogordurosa e restrita em açúcares simples e fibras insolúveis. As refeições eram isoenergéticas, isoprotéicas, sendo oferecidas em média 35 kcal/kg/dia e 1,5 g de proteína/kg/dia. Inicialmente eram oferecidos 25 a 50% do planejado e a variação de alimentos era restrita a 1 ou 2 alimentos. Com aceitação progressiva a oferta total de nutrientes era alcançada em 40 a 50 dias. Os resultados mostraram que 59% dos pacientes seguiram as orientações em relação ao consumo energético e 100% apresentaram ingestão protéica em quantidade adequada. Ao final do estudo cerca de 50% dos pacientes apresentaram índice de massa corporal dentro da faixa de normalidade e 92%, valores normais nos níveis de albumina sérica. Considerando o déficit absortivo destes pacientes, o esquema proposto mostrou-se positivo no tratamento da Síndrome do Intestino Curto.The Short Bowel Syndrome (SBS is one of the most serious and dangerous causes of malabsorption and the dietetic therapy is an important challenge to be faced. This study describes the oral dietetic therapy for all patients with Short Bowel

  1. Determinação do sistema endócrino difuso nos intestinos de três Teleostei (Pisces de água doce com hábitos alimentares diferentes

    Directory of Open Access Journals (Sweden)

    Seixas Filho José Teixeira de

    2001-01-01

    Full Text Available O objetivo do presente trabalho foi o de verificar a presença de células endócrinas nos intestinos médio e posterior, ou reto, e nos cecos pilóricos de três espécies tropicais de peixes Teleostei de água doce com hábitos alimentares diferentes: piracanjuba, (Brycon orbignyanus e piau (Leporinus friderici, onívoro; e surubim (Pseudoplatystoma coruscans, carnívoro. Para tanto, foram utilizados sete exemplares da piracanjuba, com médias de peso e comprimento-padrão de 410,16 ± 66,33 g e 27,42 ± 1,17 cm, respectivamente, 13 exemplares de piau com médias de 77,71 ± 24,31 g e 14,84 ± 1,56 cm de peso e comprimento-padrão, respectivamente; e cinco exemplares do surubim com médias de peso e comprimento-padrão de 309,91 ± 94,23 g e 32,70 ± 1,79 cm, respectivamente. Pode-se constatar a presença de células endócrinas do "tipo aberto" nos segmentos referentes aos intestinos médio e posterior das espécies onívoras e do "tipo fechado" no intestino médio e no reto da espécie carnívora. As células argirófilas foram observadas entre as células absortivas do epitélio intestinal. Os resultados permitem concluir que a presença de células endócrinas nas espécies estudadas pode indicar que estas atuam no mecanismo de controle da absorção dos nutrientes do alimento.

  2. Liver disease associated with intestinal failure in the small bowel syndrome Doença hepática associada à falência intestinal na síndrome do intestino curto

    Directory of Open Access Journals (Sweden)

    Rafael Kemp

    2006-01-01

    Full Text Available The introduction of the Total Parenteral Nutrition (TPN has given rise to a new hope in the treatment of intestinal failure (LF associated with the Short Bowel Syndrome (SBS. However, together with the TPN and the increase of survival of these patients, new problems and questions have emerged, as well as new therapeutical procedures. Taking into consideration this emerging reality, this paper has the purpose to undertake a review of current concepts and available treatments for patients with IF associated-liver disease. Although TPN provides an increase of survival of patients with intestinal failure, it is a potential source of complication such as: septicemia, hyperglycemia, venous thrombosis and liver disease. There are several hypothesis conceived to explain the liver disease associated to intestinal failure, however the only definite treatment as a potential to reverse the non-cirrhotic liver disease is the small intestine transplantation. Despite indications for intestine transplantation are not entirely defined in literature, the trend is its early indication in high-risk patients, preserving the liver integrity and preventing the eventual need of both liver and intestine transplantations altogether.A introdução da Nutrição Parenteral Total (NPT despertou uma nova esperança para o tratamento da falência intestina (FI associada a Síndrome do Intestino Curto (SIC. No entanto, junto com a NPT e o aumento da sobrevida destes pacientes, novos problemas e perguntas emergiram, assim como novas terapêuticas. Tendo em vista esta realidade emergente, o intuito deste artigo é realizar uma revisão dos conceitos atuais e dos tratamentos disponíveis para pacientes com doença hepática associada a FI. A NPT apesar de proporcionar aumento da sobrevida nos pacientes com falência intestinal é fonte potencial de complicações, como: septicemia, hiperglicemia, trombose venosa e doença hepática. Diversas são as hipóteses aventadas para

  3. Review: Non-transecting bulbar urethroplasty using buccal mucosa ...

    African Journals Online (AJOL)

    Review: Non-transecting bulbar urethroplasty using buccal mucosa. S Bugeja, S Ivaz, AV Frost, DE Andrich, AR Mundy. Abstract. Augmentation urethroplasty using oral mucosal graft has become the standard surgical treatment of long bulbar strictures. In very tight strictures the urethral plate is narrowed to the extent that an ...

  4. Laser-induced fluorescence of oral mucosa cancer

    Science.gov (United States)

    Jaliashvili, Z. V.; Medoidze, T. D.; Melikishvili, Z. G.; Gogilashvili, K. T.

    2017-10-01

    The laser-induced fluorescence (LIF) spectra have been measured for cancer-infused and control mice mucosa tissues. It was established that there is quite a difference between their LIF spectral shapes. These spectral shapes are used to express the diagnostic of different states of tissues: from normal to cancer.

  5. Helicobacter pylori and histopathological changes of gastric mucosa ...

    African Journals Online (AJOL)

    Helicobacter pylori and histopathological changes of gastric mucosa in Uganda population with varying prevalence of stomach cancer. ... Results: The severity of gastritis correlated with the presence of H. pylori in Ganda and Nyarwanda but not in Nkole. Intestinal metaplasia (IM) was observed in Nyarwanda and Nkole and ...

  6. Histomorphometric evaluation of small intestinal mucosa of red ...

    African Journals Online (AJOL)

    Histomorphometry of the small intestinal mucosa of the red jungle fowl (RJF) and commercial broiler breed (CBC) from day one to four months post-hatch were investigated. For the sake of comparison between these two breeds, the following parameters were included: the number of villi, villus surface area and the intestinal ...

  7. Taste buds in the palatal mucosa of snakes | Berkhoudt | African ...

    African Journals Online (AJOL)

    An examination of the oral mucosa of Crotalus and several Scolecophidia revealed the presence of taste buds. The taste buds in these two divergent groups of snakes are similar in appearance, and correspond to previous descriptions of gustatory organs in other reptiles. Few taste buds were present in any specimen, and ...

  8. An overview of oral mucosa condition of shisha smoker

    Directory of Open Access Journals (Sweden)

    Rahmi Amtha

    2016-08-01

    Full Text Available Shisha is a water pipe that tobacco extract and fruit scented burnt using coal. It produces the smoke through the vessel and inhaled using a hose with good taste. The culture of shisha smoking is popular in Midle East country that curently has been also entering Indonesia. The side effect of shisha smoking habit is still very rare reported. Aim of this study is to describe the oral mucosa condition of shisha user. A preliminary observasional study was conducted at several sisha cafe at South Jakarta. Under informed consent, subject with habit of tobacco and shisha smoker were included. Sociodemographic data (age, gender, duration, frequency of smoking, salivary flow rate and oral mucosa changes were documented. Eighteen subjects were recruited into this study. Most of shisha smoker was also tobacco smoker. Shisha was more practiced by male at  age (15-24 years old. The oral mucosa changes such as keratosis, melanosis, leukoedema, coated tongue, gingivitis and xerostomia were found on subject with habit of tobacco smoking habit only or both shisha and tobacco smoking. In conclusion apparently the shisha smoking habit may casue oral mucosa changes almost the same with tobacco smoking habit

  9. reactive localised inflammatory hyperplasia of the oral mucosa

    African Journals Online (AJOL)

    2009-02-02

    Feb 2, 2009 ... cases(0.9%) were those of denture irritation hyperplasia. The age ... reported to have recurred and all of them were gingival lesions. ... grade chronic irritations to the oral mucosa such as ..... J. Periodontal.1980; 51: 55-61. 7.

  10. Filaggrin gene mutations and the distribution of filaggrin in oral mucosa of patients with oral lichen planus and healthy controls.

    Science.gov (United States)

    Larsen, K R; Johansen, J D; Reibel, J; Zachariae, C; Rosing, K; Pedersen, A M L

    2017-05-01

    Lichen planus (LP) is a chronic inflammatory disease of unknown aetiology affecting the skin and oral mucosa. Oral lichenoid lesions (OLLs), like oral contact reactions, may resemble oral lichen planus (OLP) both clinically and histopathologically. As OLP and OLL are hyperkeratotic diseases and filaggrin is essential to keratinization, the distribution of filaggrin may be altered in these lesions. To investigate whether patients with OLP/OLL have (i) altered distribution of filaggrin in the oral mucosa; (ii) a higher incidence of mutations in the filaggrin gene (FLG); (iii) active dermatoses, apart from cutaneous LP, than healthy controls; and (iv) patients with OLP/OLL and a defect in the FLG have more widespread oral lesions and report more symptoms than OLP/OLL patients without a concomitant defect in the FLG. Forty-nine Caucasian patients (42 women and 7 men, mean age 61.0 ± 10.3 years), with symptomatic OLP, OLL or stomatitis, and 29 matched healthy controls underwent a clinical oral and dermatological examination, oral mucosal biopsy and filaggrin genotyping (testing for R2447X, R501X, 2282del4). Smear tests for Candida spp. were performed in all patients to exclude oral candidiasis. Immunohistochemistry were performed using poly- and monoclonal filaggrin antibodies. The immunoreactivity for filaggrin was significantly more intense in the oral mucosa in the patients with OLP/OLL compared with healthy controls (P = 0.000025). No difference was noted in the incidence of defects in the FLG and active dermatoses between patients and healthy controls. No difference was noted in extension and number of symptoms reported by patients with OLP/OLL with or without a concomitant defect in the FLG. OLP/OLL is associated with an altered distribution of filaggrin in the oral mucosa independently of defects in the FLG. Patients with OLP/OLL did not display more active dermatoses other than cutaneous LP when compared to healthy controls. © 2016 European Academy of

  11. Validation of methylation biomarkers that distinguish normal colon mucosa from cancer patients from normal colon mucosa of patients without cancer

    Science.gov (United States)

    Cesaroni, Matteo; Powell, Jasmine; Sapienza, Carmen

    2014-01-01

    We have validated differences in DNA methylation levels of candidate genes previously reported to discriminate between normal colon mucosa of colon cancer patients and normal colon mucosa of individuals without cancer. Here, we report that CpG sites in 16 of the 30 candidate genes selected show significant differences in mean methylation level in normal colon mucosa of 24 cancer patients and 24 controls. A support vector machine trained on these data and data for an additional 66 CpGs yielded an 18-gene signature, composed of 10 of the validated candidate genes plus eight additional candidates. This model exhibited 96% sensitivity and 100% specificity in a 40-sample training set and classified all eight samples in the test set correctly. Moreover, we found a moderate-strong correlation (Pearson coefficients r=0.253-0.722) between methylation levels in colon mucosa and methylation levels in peripheral blood for seven of the 18 genes in the support vector model. These seven genes, alone, classified 44 of the 48 patients in the validation set correctly and five CpGs selected from only two of the seven genes classified 41 of the 48 patients in the discovery set correctly. These results suggest that methylation biomarkers may be developed that will, at minimum, serve as useful objective and quantitative diagnostic complements to colonoscopy as a cancer-screening tool. These data also suggest that it may be possible to monitor biomarker methylation levels in tissues collected much less invasively than by colonoscopy. PMID:24806665

  12. MZC Gel Inhibits SHIV-RT and HSV-2 in Macaque Vaginal Mucosa and SHIV-RT in Rectal Mucosa.

    Science.gov (United States)

    Calenda, Giulia; Villegas, Guillermo; Barnable, Patrick; Litterst, Claudia; Levendosky, Keith; Gettie, Agegnehu; Cooney, Michael L; Blanchard, James; Fernández-Romero, José A; Zydowsky, Thomas M; Teleshova, Natalia

    2017-03-01

    The Population Council's microbicide gel MZC (also known as PC-1005) containing MIV-150 and zinc acetate dihydrate (ZA) in carrageenan (CG) has shown promise as a broad-spectrum microbicide against HIV, herpes simplex virus (HSV), and human papillomavirus. Previous data show antiviral activity against these viruses in cell-based assays, prevention of vaginal and rectal simian-human immunodeficiency virus reverse transcriptase (SHIV-RT) infection, and reduction of vaginal HSV shedding in rhesus macaques and also excellent antiviral activity against HSV and human papillomavirus in murine models. Recently, we demonstrated that MZC is safe and effective against SHIV-RT in macaque vaginal explants. Here we established models of ex vivo SHIV-RT/HSV-2 coinfection of vaginal mucosa and SHIV-RT infection of rectal mucosa in macaques (challenge of rectal mucosa with HSV-2 did not result in reproducible tissue infection), evaluated antiviral activity of MZC, and compared quantitative polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay readouts for monitoring SHIV-RT infection. MZC (at nontoxic dilutions) significantly inhibited SHIV-RT in vaginal and rectal mucosas and HSV-2 in vaginal mucosa when present during viral challenge. Analysis of SHIV-RT infection and MZC activity by 1-step simian immunodeficiency virus gag quantitative RT-PCR and p27 enzyme-linked immunosorbent assay demonstrated similar virus growth dynamics and MZC activity by both methods and higher sensitivity of quantitative RT-PCR. Our data provide more evidence that MZC is a promising dual compartment multipurpose prevention technology candidate.

  13. Serous otitis media and immunological reactions in the middle ear mucosa

    NARCIS (Netherlands)

    van der Baan, S.; Seldenrijk, C. A.; Henzen-Logmans, S. C.; Drexhage, H. A.

    1988-01-01

    An immunohistochemical study was performed on the middle ear mucosa of 21 children with chronic serous otitis media (SOM). In 86% of the patients a highly organized lymphatic tissue was found in the middle ear mucosa which can be regarded as part of the mucosa associated lymphoid tissue (MALT).

  14. Verruca vulgaris of the buccal mucosa: A case report

    Directory of Open Access Journals (Sweden)

    Aastha Mattoo

    2018-01-01

    Full Text Available Oral verruca vulgaris is caused by human papillomavirus (HPV infection. Verruca vulgaris most frequently occurs on the fingers, toes, soles, and dorsal surfaces of hands and is mostly asymptomatic. Varieties of verrucous and papillary lesions affect the skin as well as oral mucosa which may be either benign or reactive. Common wart is one of the most commonly observed skin growths and a lesion of childhood. Intraoral warts can occur at any age with equal incidence in both genders but are most commonly seen in the third to fifth decade. It is found commonly on the palate followed by lip, tongue, buccal mucosa, and rarely seen on gingiva. Surgical excision with adequate margins is the treatment of choice.

  15. Two Cases of Bacteremia Due to Roseomonas mucosa.

    Science.gov (United States)

    Kim, Yu Kyung; Moon, Jung Suk; Song, Kyung Eun; Lee, Won Kil

    2016-07-01

    Roseomonas is a genus of pink-pigmented nonfermentative bacilli. These slow-growing, gram-negative cocobacilli form pink-colored colonies on sheep blood agar. They differ from other pink-pigmented nonfermenters, including Methylobacterium, in morphology, biochemical characteristics, and DNA sequence. Roseomonas strains are rarely isolated in clinical laboratories; therefore, we report two cases in order to improve our ability to identify these pathogens. We isolated two strains of Roseomonas mucosa from the venous blood cultures of two patients, an 84-yr-old woman with common bile duct obstruction and a 17-yr-old male with acute myeloid leukemia who had an indwelling central-venous catheter for chemotherapy. The isolated strains were confirmed as R. mucosa by 16S rRNA sequencing.

  16. Procedimiento para hemorroides con prolapso de mucosa: técnica de Longo. Reporte preliminar

    Directory of Open Access Journals (Sweden)

    Luis Augusto Borda Mederos

    2009-10-01

    Full Text Available La hemorroidectomía es una excelente técnica para el tratamiento de las hemorroides grado III y IV pero va asociada invariablemente a dolor postoperatorio. La mucosectomía con stapler circular- procedimiento para prolapso y hemorroides- (PPH ha demostrado menor dolor y recuperación más rápida. No se ha encontrado reportes de la técnica en nuestro país. Objetivo: Evaluar la eficacia y seguridad del PPH en pacientes portadores de hemorroides grado III y IV con prolapso de mucosa rectal. Material y método: Estudio retrospectivo tipo serie de casos de pacientes operados de hemorroides grado III - IV con prolapso de mucosa rectal, entre diciembre de 1998 hasta diciembre 2007 en el Hospital de Emergencias Grau - EsSalud, Hospital G. Almenara I. - EsSalud y Clínica San Borja. Resultados: Se estudiaron 18 pacientes (13 hombres, 5 mujeres. La hospitalización promedio fue de 1,2 días. El tiempo operatorio promedio fue de 17,5 minutos. El 94,4% de los pacientes no refirió dolor o éste fue leve. Un paciente presentó sangrado postoperatorio importante a nivel de la línea de grapas que prolongó su hospitalización, una paciente presentó dolor intenso y una paciente presentó una fístula rectovaginal. Conclusiones: La técnica de PPH es efectiva para tratar la sintomatología de las hemorroides grado III y IV con prolapso mucoso a corto y mediano plazo.(Rev Med Hered 2009;20:190-194.

  17. Educating Normal Breast Mucosa to Prevent Breast Cancer

    Science.gov (United States)

    2016-12-01

    immune system to maintain epithelial integrity. In this study our goal was to study the immune subsets associated with breast mucosa and develop the...into the mammary gland. Specific Aim 3: Determine an optimal oral vaccine approach able to minimize hyperplasia . 5 287 288 289 290 291 292...colonization, but also regulating homeostasis of the epithelial layer. As a part of the mucosal immune system, the mammary gland may have characteristic

  18. Histopathological findings in the oral mucosa of celiac patients

    Directory of Open Access Journals (Sweden)

    Elena Bardellini

    2014-02-01

    Full Text Available Background: Celiac disease (CD is an immune-mediated enteropathy triggered by the ingestion of gluten in genetically susceptible subjects. Although the small intestinal mucosa is the main site of the gut's involvement in CD, other mucosal surfaces belonging to the gastrointestinal tract and the gut-associated lymphoid tissue are known to be affected. Aim: Assuming that the oral mucosa could reflect the histopathological inflammatory alterations of the intestine in CD patients, this study wishes to assess the pattern of T-cell subsets in the oral mucosa of young adults with CD. Methods: A group of 37 patients (age range 20-38 years; female: male ratio 28:9 with CD were enrolled. Out of 37 patients, 19 patients (group A followed a gluten free diet (GFD -2 patients from less than one year; 6 patients between 1 and 5 years; 11 patients more than 5 years- while 18 patients (group B were still untreated. Fifteen healthy volunteers (age range 18-35 years, female: Male ratio 11:4 served as controls for the CD patients. Ethical approval for the research was granted by the Ethics Committee. Biopsy specimens were taken from normal looking oral mucosa. The immunohistochemical investigation was performed with monoclonal antibodies to CD3, CD4, CD8, and γδ-chains T cell receptor (TCR. Results: The T-lymphocytic inflammatory infiltrate was significantly (p < 0.0001 increased in group B (both compared with group A and with the control group. Conclusion: This study confirms the oral cavity to be a site of involvement of CD and its possible diagnostic potentiality in this disease.

  19. Abundance of Enterobacteriaceae in the colon mucosa in diverticular disease.

    Science.gov (United States)

    Linninge, Caroline; Roth, Bodil; Erlanson-Albertsson, Charlotte; Molin, Göran; Toth, Ervin; Ohlsson, Bodil

    2018-02-15

    To compare gut bacterial diversity and amount of Enterobacteriaceae in colonic mucosa between patients with and without diverticular disease (DD). Patients in a stable clinical condition with planned elective colonoscopy were included. Blood samples and colon mucosa biopsies were collected at the colonoscopy. Study questionnaires including questions about gastrointestinal symptoms were completed by the patients and physicians. DNA from mucosa samples was isolated and the amount of Enterobacteriaceae was estimated using PCR assay. Terminal restriction fragment length polymorphism was applied to assess microbial diversity. Diversity was estimated by calculations of richness (number of terminal restriction fragments) and Shannon-Wiener and Simpson's indices. A total of 51 patients were included, 16 patients with DD [68 (62-76) years] and 35 controls [62 (40-74) years] without any diverticula. Patients with DD had significantly higher levels of Enterobacteriaceae than those without DD ( P = 0.043), and there was an inverse relationship between the amount of Enterobacteriaceae and the Simpson's index (rs = -0.361, P = 0.033) and the Shannon-Wiener index (rs = -0.299, P = 0.081). The Simpson's index ( P = 0.383), Shannon-Wiener index ( P = 0.401) or number of restrictions fragments ( P = 0.776) did not differ between DD and controls. The majority of patients experienced gastrointestinal symptoms, and 22 patients (43.1%) fulfilled the criteria for irritable bowel syndrome, with no difference between the groups ( P = 0.212). Demography, socioeconomic status, lifestyle habits, inflammatory biomarkers, or symptoms were not related to the amount of Enterobacteriaceae or bacterial diversity. Patients with DD had higher amount of Enterobacteriaceae in the colon mucosa compared to patients without diverticula.

  20. Oral mucosa tissue response to titanium cover screws.

    Science.gov (United States)

    Olmedo, Daniel G; Paparella, María L; Spielberg, Martín; Brandizzi, Daniel; Guglielmotti, María B; Cabrini, Rómulo L

    2012-08-01

    Titanium is the most widely used metal in dental implantology. The release of particles from metal structures into the biologic milieu may be the result of electrochemical processes (corrosion) and/or mechanical disruption during insertion, abutment connection, or removal of failing implants. The aim of the present study is to evaluate tissue response of human oral mucosa adjacent to titanium cover screws. One hundred fifty-three biopsies of the supra-implant oral mucosa adjacent to the cover screw of submerged dental implants were analyzed. Histologic studies were performed to analyze epithelial and connective tissue as well as the presence of metal particles, which were identified using microchemical analysis. Langerhans cells, macrophages, and T lymphocytes were studied using immunohistochemical techniques. The surface of the cover screws was evaluated by scanning electron microscopy (SEM). Forty-one percent of mucosa biopsies exhibited metal particles in different layers of the section thickness. Particle number and size varied greatly among specimens. Immunohistochemical study confirmed the presence of macrophages and T lymphocytes associated with the metal particles. Microchemical analysis revealed the presence of titanium in the particles. On SEM analysis, the surface of the screws exhibited depressions and irregularities. The biologic effects seen in the mucosa in contact with the cover screws might be associated with the presence of titanium or other elements, such as aluminum or vanadium. The potential long-term biologic effects of particles on soft tissues adjacent to metallic devices should be further investigated because these effects might affect the clinical outcome of the implant.

  1. Untersuchungen zur Regeneration des Hinterendes bei Anaitides mucosa (Polychaeta, Phyllodocidae)

    Science.gov (United States)

    Röhrkasten, A.

    1983-06-01

    Caudal regeneration was investigated in decerebrate Anaitides mucosa and in brain-intact individuals. Both groups show an identical capacity to regenerate lost caudal segments. Furthermore there is no difference in males and females. Low temperature (5 °C) inhibits the regeneration of caudal segments, but it is necessary for normal oogenesis. Under conditions of high temperature (15 °C), caudal regeneration is very extensive. At the same time degeneration of most oocytes occurs.

  2. Paraneoplastic disorders of hair, nails, oral mucosa and pigmentation

    Directory of Open Access Journals (Sweden)

    Ayşe Kavak

    2013-12-01

    Full Text Available In this section, paraneoplastic entites of hair, nail, oral mucosa and pigmentation changes have been discussed. Some skin findings are “strong” indicator of a malignancy whereas others are not. Readers will encounter some “coincidental” or “common” entities as well as more “severe” changes for a paraneoplastic sign. In addition, it is crucial that some paraneoplastic lesions may predict for a recurrence of malignancy.

  3. Keratocyst of the buccal mucosa: is it odontogenic?

    Science.gov (United States)

    Ide, Fumio; Kikuchi, Kentaro; Miyazaki, Yuji; Mishima, Kenji; Saito, Ichiro; Kusama, Kaoru

    2010-11-01

    Odontogenic keratocyst (OKC) of the buccal mucosa, the diagnosis of which is based on subjective histologic evaluation, is a controversial entity of questionable existence. This report describes 2 rare cases of parakeratinized cyst arising from the buccal mucosa. Case 1 was a 60-year-old man with a 3-cm cyst and case 2 was a 16-year-old boy with a microcyst incidentally discovered on histology. Both lesions were essentially identical in histologic appearance and immunophenotype to intraosseous and gingival OKC, but they were clearly different from orthokeratinized odontogenic cysts and buccal mucosal epidermoid cysts. Step sections failed to reveal any kind of odontogenic tissue or skin adnexa in the cyst wall. These microscopic characteristics reflexively lead to the diagnosis of OKC, if the extragingival occurrence in the buccal mucosa cannot be considered. An alternative nonodontogenic origin includes a keratocyst of the skin, ie, an unusual mucosal presentation of cutaneous keratocyst. Because its true nature, either odontogenic or epidermal, cannot be conclusively proven at this time, we propose a more descriptive and noncommittal term, "mucosal keratocyst," for a particular cyst in a buccal location that is morphologically indistinguishable from OKC. Copyright © 2010 Mosby, Inc. All rights reserved.

  4. Interaction of Mycobacterium tuberculosis with human respiratory mucosa.

    Science.gov (United States)

    Middleton, A M; Chadwick, M V; Nicholson, A G; Dewar, A; Groger, R K; Brown, E J; Ratliff, T L; Wilson, R

    2002-01-01

    Endobronchial infection is associated with pulmonary tuberculosis in the majority of cases. We have investigated the adherence of Mycobacterium tuberculosis to the human respiratory mucosa. Organ cultures constructed with human tissue were infected with M. tuberculosis in the presence or absence of mycobacterial fibronectin attachment cell surface proteins and examined by scanning electron microscopy. M. tuberculosis adhered mainly to extracellular matrix (ECM) in areas of mucosal damage, but not to ciliated mucosa, intact extruded cells, basement membrane or collagen fibres. Bacteria also adhered to fibrous but not globular mucus and occasionally to healthy unciliated mucosa, open tight junctions and to extruded cells that had degenerated, exposing their contents. There was a significant reduction (pprotein (FAP) and M. bovis antigen 85B protein, in a concentration dependent manner. The combined effect of FAP and antigen 85B protein was significantly greater than either protein alone. Bacterial adherence to fibrous mucus was not influenced by fibronectin. We conclude that M. tuberculosis adheres to ECM in areas of mucosal damage at least in part via FAP and antigen 85B protein.

  5. Delivery of bioactive peptides and proteins across oral (buccal) mucosa.

    Science.gov (United States)

    Senel, S; Kremer, M; Nagy, K; Squier, C

    2001-06-01

    The identification of an increasing array of highly potent, endogenous peptide and protein factors termed cytokines, that can be efficiently synthesized using recombinant DNA technology, offers exciting new approaches for drug therapy. However, the physico-chemical and biological properties of these agents impose limitations in formulation and development of optimum drug delivery systems as well as on the routes of delivery. Oral mucosa, including the lining of the cheek (buccal mucosa), floor of mouth and underside of tongue (sublingual mucosa) and gingival mucosa, has received much attention in the last decade because it offers excellent accessibility, is not easily traumatized and avoids degradation of proteins and peptides that occurs as a result of oral administration, gastrointestinal absorption and first-pass hepatic metabolism. Peptide absorption occurs across oral mucosa by passive diffusion and it is unlikely that there is a carrier-mediated transport mechanism. The principal pathway is probably via the intercellular route where the major permeability barrier is represented by organized array of neutral lipids in the superficial layers of the epithelium. The relative role of aqueous as opposed to the lipid pathway in drug transport is still under investigation; penetration is not necessarily enhanced by simply increasing lipophilicity, for other effects, such as charge and molecular size, also play an important role in absorption of peptide and protein drugs. Depending on the pharmacodynamics of the peptides, various oral mucosal delivery systems can be designed. Delivery of peptide/protein drugs by conventional means such as solutions has some limitations. The possibility of excluding a major part of drug from absorption by involuntary swallowing and the continuous dilution due to salivary flow limits a controlled release. However these limitations can be overcome by adhesive dosage forms such as gels, films, tablets, and patches. They can localize the

  6. Validation of methylation biomarkers that distinguish normal colon mucosa of cancer patients from normal colon mucosa of patients without cancer.

    Science.gov (United States)

    Cesaroni, Matteo; Powell, Jasmine; Sapienza, Carmen

    2014-07-01

    We have validated differences in DNA methylation levels of candidate genes previously reported to discriminate between normal colon mucosa of patients with colon cancer and normal colon mucosa of individuals without cancer. Here, we report that CpG sites in 16 of the 30 candidate genes selected show significant differences in mean methylation level in normal colon mucosa of 24 patients with cancer and 24 controls. A support vector machine trained on these data and data for an additional 66 CpGs yielded an 18-gene signature, composed of ten of the validated candidate genes plus eight additional candidates. This model exhibited 96% sensitivity and 100% specificity in a 40-sample training set and classified all eight samples in the test set correctly. Moreover, we found a moderate-strong correlation (Pearson coefficients r = 0.253-0.722) between methylation levels in colon mucosa and methylation levels in peripheral blood for seven of the 18 genes in the support vector model. These seven genes, alone, classified 44 of the 48 patients in the validation set correctly and five CpGs selected from only two of the seven genes classified 41 of the 48 patients in the discovery set correctly. These results suggest that methylation biomarkers may be developed that will, at minimum, serve as useful objective and quantitative diagnostic complements to colonoscopy as a cancer-screening tool. These data also suggest that it may be possible to monitor biomarker methylation levels in tissues collected much less invasively than by colonoscopy. ©2014 American Association for Cancer Research.

  7. Effect of ochratoxin A on the intestinal mucosa and mucosa-associated lymphoid tissues in broiler chickens.

    Science.gov (United States)

    Solcan, Carmen; Pavel, Geta; Floristean, Viorel Cezar; Chiriac, Ioan Sorin Beschea; Şlencu, Bogdan Gabriel; Solcan, Gheorghe

    2015-03-01

    The immunotoxic effect of ochratoxin A (OTA) on the intestinal mucosa-associated lymphoid tissue and its cytotoxic action on the intestinal epithelium were studied in broiler chickens experimentally treated with the toxin. From the 7th day of life, 80 male broiler chickens (Ross 308) were randomly divided into four groups of 20 birds each. The three experimental groups (E1-3) were treated with OTA for 28 days (E1: 50 μg/kg body weight [bw]/day; E2: 20 μg/kg bw/day; E3: 1 μg/kg bw/day) and the fourth group served as control. Histological examination of the intestinal mucosa and immunohistochemical staining for identification of CD4+, CD8+, TCR1 and TCR2 lymphocytes in the duodenum, jejunum and ileocaecal junction were performed, and CD4+/CD8+ and TCR1/TCR2 ratios were calculated. OTA toxicity resulted in decreased body weight gain, poorer feed conversion ratio, lower leukocyte and lymphocyte count, and altered intestinal mucosa architecture. After 14 days of exposure to OTA, immunohistochemistry showed a significant reduction of the lymphocyte population in the intestinal epithelium and the lamina propria. After 28 days of exposure, an increase in the CD4+ and CD8+ values in both the duodenum and jejunum of chickens in Groups E1 and E2 was observed, but the TCR1 and TCR2 lymphocyte counts showed a significant reduction. No significant changes were observed in Group E3. The results indicate that OTA induced a decrease in leukocyte and lymphocyte counts and was cytotoxic to the intestinal epithelium and the mucosa-associated lymphoid tissue, altering the intestinal barrier and increasing susceptibility to various associated diseases.

  8. Obstrucción intestinal por persistencia de remanente del conducto vitelino en un paciente pediátrico. Presentación de un caso.

    OpenAIRE

    Jorge Leguen Berwich; Yoan Varela Rodríguez; Idalmis Céspedes Marín

    2016-01-01

    El Divertículo de Meckel, anomalía congénita más frecuente del intestino delgado, resulta de una obliteración incompleta del conducto onfalomesentérico. Una forma clínica de presentación es la obstrucción intestinal por persistencia de remanente del conducto onfalomesentérico. Se presenta el caso de un paciente de 2 años que tuvo dolor en fosa iliaca derecha, vómitos, distensión abdominal, irritabilidad y disminución de los ruidos hidroaéreos. Se realizaron estudios diagnósticos y se dec...

  9. Prevención del daño intestinal mediante la utilización de un antagonista del factor de activación plaquetaria en el transplante intestinal

    Directory of Open Access Journals (Sweden)

    Carlos Vaquero Puerta

    1997-04-01

    Full Text Available Se realiza un estudio comparativo en ratas singénicas de la cepa Wistar con objeto de valorar los efectos de preservación del injerto al utilizar el antagonista del factor de activación plaquetaria, WEB-2086. En la valoración ultraestructural se ha podido comprobar los efectos de esta sustancia al nivel de las células epiteliales de intestino trasplantadoA comparative study was carried out in syngeneic Wistar rats with the aim of assess the effects of the preservation of grafts when using the antagonist of the platelet activating factor, WEB-2086. The effects of this substance at the level of epithelial cells of the transplantated intestine were proved in the ultrastructural assessment

  10. Study of the density of ganglion cells in the terminal bowel of rats with anorectal malformations Estudo da densidade das células ganglionares no intestino terminal de ratos portadores de anomalia anorretal

    Directory of Open Access Journals (Sweden)

    Maurício Macedo

    2007-12-01

    Full Text Available PURPOSE: To study the ganglion cells (GC in the terminal bowel of rats with ethylenethiourea (ETU induced anorectal malformations (ARM. METHODS: The animals were divided into three groups: Group A - normal fetuses from pregnant rats that were not administered ETU; Group B - fetuses without ARM born from pregnant rats that were administered ETU and Group C - fetuses with ARM born from pregnant rats that received ETU. ETU was administered on the 11th day of pregnancy at the dose of 125 mg/kg body weight by gastric gavage. The rats had cesarean section on the 21st day of gestation. The fetuses’ terminal bowel tissue was analyzed by immunohistochemistry to demonstrate ganglion cells. RESULTS: Statistically significant differences were found between groups A, B and C regarding ganglion cell densities. Group A had the highest cell density, followed by Group B and the lowest density was found in Group C. CONCLUSION: Ganglion cell densities are decreased in the terminal bowel of rats with ARM.OBJETIVO: Estudar as células ganglionares (CG no intestino terminal de ratos portadores de anomalia anorretal (AAR induzida pela etilenotiouréia (ETU. MÉTODOS: Os animais foram distribuídos em três grupos: Grupo A - fetos normais, obtidos de ratas grávidas às quais não foi administrada ETU; Grupo B - fetos não portadores de AAR obtidos de ratas grávidas às quais foi administrada ETU e Grupo C - fetos portadores de AAR obtidos de ratas grávidas às quais foi administrada ETU. A ETU foi administrada no décimo primeiro dia de gestação na dose de 125 mg/Kg, por gavagem. As ratas foram submetidas à laparotomia e histerotomia para retirada dos fetos no vigésimo primeiro dia de gestação. O intestino terminal dos fetos foi retirado e analisado por imunohistoquímica para pesquisa de CG. RESULTADOS: Foram encontradas diferenças estatisticamente significantes entre os grupos A, B e C quanto à densidade de CG. O grupo A apresentou a maior densidade

  11. Alterações morfométricas da musculatura dos músculos longitudinal e circular de ratos submetidos à criação de piloros no intestino delgado

    Directory of Open Access Journals (Sweden)

    Cícero de Lima Rena

    Full Text Available OBJETIVO: Realizar um estudo morfométrico comparativo da musculatura longitudinal e circular do intestino delgado de ratos submetidos à construção cirúrgica de um e de dois piloros no intestino delgado utilizando-se da técnica de criação de piloros proposta por Rena et al. MÉTODO: Foram utilizados 52 ratos Wistar machos divididos em três grupos. O Grupo A, destacado como controle, composto de 10 animas, cada um forneceu 20mm de segmento de intestino para o estudo. Os animais do Grupo B, composto de 32 animais, foram submetidos à construção de dois piloros, um a 100mm e outro a 150mm da válvula ileocecal e os animais do grupo C, composto de 10 animais, foram submetidos à construção de um piloro a 100mm da mesma. A eutanásia ocorreu no décimo dia. A morfometria das camadas musculares longitudinal e circular acima e abaixo dos piloros foi estudada com utilização do microscópico modelo "Axiostar plus" conectado à câmera "Axioncam Version 5.05.10" com objetiva X5 / 0,12 no o programa "AxioVision 3.1.2.1". RESULTADOS: As alças apresentaram aumento da espessura da camada muscular acima e abaixo do ponto da operação. O estudo morfométrico comparativo das camadas musculares longitudinal e circular mostrou aumento significativo em comparação ao controle, não demonstrando significância entre os grupos B e C. CONCLUSÃO: Os resultados deste estudo revelaram importante aumento de espessura das camadas musculares, porém, menos acentuados que aqueles descritos na literatura quando foram realizados em animais submetidos à estenose fixa. Esses dados indicam que a construção operatória de piloros pela técnica utilizada promove alterações musculares de menor monta, possivelmente, pela função valvular do piloro.

  12. Submucosa de intestino delgado no reparo de defeito em parede abdominal de ratos Small intestinal submucosa to repair anterior abdominal wall defect in rats

    Directory of Open Access Journals (Sweden)

    Fernando Hintz Greca

    2004-10-01

    Full Text Available OBJETIVO: Comparar a biocompatibilidade da tela de polipropileno e do enxerto de submucosa intestinal de suínos, quando usados para o reparo de defeito criado na parede anterior de abdomen de ratos, que envolveu toda a sua espessura e extensão. MÉTODO: Vinte ratos Wistar foram divididos em dois grupos de 10 animais. Um defeito quadrangular, com 3,5cm de lado foi criado cirurgicamente na parede abdominal anterior dos 20 ratos. No primeiro grupo(grupo 1 este defeito foi reparado com submucosa de intestino delgado (SID, e no segundo grupo(grupo 2 com tela de polipropileno. Após 30 dias, os animais foram sacrificados. Além da avaliação macroscópica dos enxertos, realizou-se um estudo tensiométrico e microscópico. RESULTADOS: Os animais de ambos os grupos apresentaram aderências entre o implante e as estruturas intra-abdominais, todavia no grupo com a tela de polipropileno observou-se maior número de aderências entre a tela e as alças intestinais. A força máxima de ruptura foi significantemente maior no grupo de animais com a tela de polipropileno, todavia se corrigida pela espessura do enxerto, a tensão máxima foi maior no enxerto de submucosa porcina que na tela de polipropileno. A mesotelisação foi significativamente mais intensa no grupo da submucosa, assim como a deposição de colágeno. As reações granulomatosas de corpo estranho e inflamação crônica também foram mais intensas no grupo 1. CONCLUSÃO: A SID monstrou ser uma alternativa ao enxerto sintético no reparo de grandes defeitos da parede abdominal em ratos, com melhor mesotelisação e deposição de colágeno, sem aumento no número de complicações. A submucosa apresentou maior resistência por mm², apesar de ter se mostrado menos resistente quando considerada a força maneira absoluta.PURPOSE: The aim of the present study was compare the biocompatibility of a polypropylene mesh and a patch of porcine small intestinal submucosa (SIS when used to repair a

  13. Hemorragia maciça do intestino grosso: o que está ao nosso alcance? Massive large bowel bleeding: what is within our reach?

    Directory of Open Access Journals (Sweden)

    Júlio César M Santos Jr.

    2010-06-01

    Full Text Available A forma de avaliar e lidar com a hemorragia aguda digestiva baixa tem sido modificado com os recentes desenvolvimentos de novas técnicas e aparelhos. O nosso objetivo, por esse manuscrito, é demonstrar com simplicidade uma forma de condução dos pacientes com hemorragia digestiva baixa aguda, sobretudo para os sangramentos que parecem mais graves, principalmente quando nos faltam os recursos das avançadas tecnologias atuais. Doenças localizadas no intestino grosso respondem por ¼ dos casos de hemorragias digestivas que são motivos para admissão hospitalar e têm como principais agentes etiológicos os divertículos, na moléstia diverticular, e as alterações vasculares, nas angiodisplasias. Na grande maioria das vezes o sangramento que pode parecer abundante cessa espontaneamente. Os distúrbios circulatórios graves não são comuns e os sinais mais frequentes são a queda do valor da hemoglobina observada na metade dos pacientes, e algumas alterações hemodinâmicas como a variação pressórica postural que pode ser vista em até 30% dos casos; a síncope em 10% e alguma forma de colapso circulatório, em 9%. Contudo, a faixa etária em que ocorre, as condições da senilidade, as doenças eventualmente associadas e a falta de recursos materiais e humanos para lidar com esse tipo de problema são os motivos que mais causam preocupação. A abordagem clínica simples com uma história bem elaborada; o exame físico com atenção e objetividade, a inclusão do exame proctológico, a obrigatória disponibilidade de aparelho para a coloscopia e os conhecimentos básicos sobre o evento formam o conjunto necessário e, na maioria das vezes, suficiente para o correto desempenho profissional na elaboração dos cuidados que devem ser dados a esses pacientes.The evaluation and management of the acute lower digestive hemorrhage has been modified with the recent development of new techniques and devices. The aim of this manuscript was to

  14. Multiple mucous retention cysts of the oral mucosa.

    Science.gov (United States)

    Tal, H; Altini, M; Lemmer, J

    1984-12-01

    While mucoceles of the oral mucosa are relatively common, multiple mucous retention cysts have not previously been reported. In this article two such cases, in which numerous minor salivary gland ducts had dilated to the point of cyst formation, are described. The number of individual cysts exceeded 100 in each case. Since it is clear that these cysts formed as a result of dilatation of salivary ducts, it would seem that either the ducts were blocked by altered secretion or there was an acquired or congenital weakness in their structure.

  15. Extensive amalgam tattoo on the alveolar-gingival mucosa.

    Science.gov (United States)

    Galletta, Vivian C; Artico, Gabriela; Dal Vechio, Aluana M C; Lemos, Celso A; Migliari, Dante A

    2011-01-01

    Amalgam tattoos are common exogenous pigmented lesions of the oral mucosa occurring mainly by inadvertent placement of amalgam particles into soft tissues. The diagnosis of amalgam tattoo is simple, usually based on clinical findings associated with presence or history of amalgam fillings removal. Intraoral X-rays may be helpful in detecting amalgam-related radiopacity. In cases where amalgam tattoo cannot be differentiated from other causes of oral pigmentation, a biopsy should be performed. This article deals with an extensive amalgam tattoo lesion which required a biopsy for a definitive diagnosis.

  16. Distribution of some elements in human colon mucosa

    International Nuclear Information System (INIS)

    Drashkovich, R.J.

    1985-01-01

    The contents of Co, Zn, Fe, Cr and Sb were determined in human colon mucosa as a function of pathalogical alterations during development of colitis Chronica, Colitis Ulcerosa, Adenoma Tubulare and Adenocarcinoma. The sample (0.00023-0.00087 kg in weight) from 80 patients were taken during rectosigmoidoscopy by teflon coated forceps and were deep frozen (T=244 deg. K) and liophilysed. A thermal neutron fluxes 0.54-1.85x10 17 n/m 2 .s for 3 days and 4096-channel analyser with a Ge(Li) detector

  17. Investigation of phosphatidylcholine enhancing FITC-insulin across buccal mucosa by confocal laser scanning microscopy

    Science.gov (United States)

    Tian, Weiqun; Su, Li; Zeng, Shaoqun; Luo, Qingming; Gao, Qiuhua; Xu, Huibi

    2002-04-01

    The aim was to characterize the transport of fluorescein isothiocyanate (FITC)-labeled dextran and insulin with different resoluble compounds for peptides and proteins through buccal mucosa. The penetration rate of insulin molecules through porcine buccal mucosa (a nonkeratinized epithelium, comparable to human buccal mucosa) was investigated by measuring transbuccal fluxes and by analyzing the distribution of the fluorescent probe in the rabbit buccal mucosa epithelium, using confocal laser scanning microscopy for visualizing permeation pathways. The confocal images of the distribution pattern of FITC-dextran and FITC-insulin showed that the paracellular route is the major pathway of FITC-dextran through buccal mucosa epithelium, the intra-cellular route is the major pathway of FITC-insulin through buccal mucosa epithelium. The permeation rate can be increased by co-administration of soybean phosphatidylcholine (SPC).

  18. Relation between radiotherapy-induced acute injury of mucosa of nasopharyngeal carcinoma and p53 polymorphisms

    International Nuclear Information System (INIS)

    Wang Changsheng; Xiao Shaowen; Zhang Shanwen

    2007-01-01

    Objective: To explore the relation between p53 genetic polymorphisms and radiotherapy-induced acute injury of mucosa of oral cavity mucosa. Methods: The total of 56 patients with NPC treated by radiotherapy alone or with chemoradiotherapy synchronically were genotyped for the p53 codon 72 pro-Arg SNP using PCR-RFLP assays, and were ranked according to the acute injury of oral cavity mucosa. Results: There was no difference in acute injury of oral cavity mucosa between the p53 Pro allele carriers and the other carriers (P>0.05); the high single dose (P<0.01) and concomitant chemoradiotherapy (P<0.05) resulted in increase in acute injury of oral cavity mucosa. Conclusion: Those results suggest that p53 SNP may not associate with radiotherapeutic acute injury of oral cavity mucosa. (authors)

  19. A mucosa-mimetic material for the mucoadhesion testing of thermogelling semi-solids.

    Science.gov (United States)

    da Silva, Jéssica Bassi; Khutoryanskiy, Vitaliy V; Bruschi, Marcos L; Cook, Michael T

    2017-08-07

    Mucosa-mimetic materials are synthetic substrates which aim to replace animal tissue in mucoadhesion experiments. One potential mucosa-mimetic material is a hydrogel comprised of N-acryloyl-d-glucosamine and 2-hydroxyethylmethacrylate, which has been investigated as a surrogate for animal mucosae in the mucoadhesion testing of tablets and solution formulations. This study aims to investigate the efficacy of this mucosa-mimetic material in the testing of thermogelling semi-solid formulations, which transition from solution to gel upon warming. Two methods for assessing mucoadhesion have been used; tensile testing and a flow-through system, which allow for investigation under dramatically different conditions. It was found that the mucosa-mimetic material was a good surrogate for buccal mucosa using both testing methods. This material may be used to replace animal tissue in these experiments, potentially reducing the number of laboratory animals used in studies of this type. Copyright © 2017 Elsevier B.V. All rights reserved.

  20. Historia natural de la infección por el virus del papiloma humano: una actualización

    OpenAIRE

    González Martínez, Gerardo; Núñez Troconis, José

    2014-01-01

    Durante los últimos años, se han sucedido grandes avances en nuestro entendimiento acerca de la biología e historia natural del Virus del Papiloma Humano (VPH). La mayoría de las infecciones por papiloma virus son transmitidas por un contacto cercano bien sea de piel a piel o mucosa a mucosa. La relación sexual con penetración no es un requerimiento para la transmisión del VPH. Las infecciones orales y digitales por VPH ocurren, y existe evidencia de que el contacto digital-genital y genital-...

  1. Phase II Clinical Trial of Intraoral Grafting of Human Tissue Engineered Oral Mucosa

    Science.gov (United States)

    2017-10-01

    treatment, EVPOME (Group 1), or standard of care, the palatal oral mucosa (POM) graft (Group 2). The study population will include non- smoking adults...nor prevents microbial infection, fluid loss, and foreign material contamination and relapse secondary to wound contracture. Oral mucosa is in... smoking subjects (ages 18 and older) in need of additional keratinized oral mucosa and provided recruitment materials to local dentists. This resulted

  2. Squamous cell carcinoma of buccal mucosa: An analysis of prognostic factors

    OpenAIRE

    Saurabh Bobdey; Jignasa Sathwara; Aanchal Jain; Sushma Saoba; Ganesh Balasubramaniam

    2018-01-01

    Introduction: Carcinoma of the buccal mucosa is the most common oral cavity cancer in the Indian subcontinent. The aim of this study was to analyze the outcome and evaluate prognostic factors in surgically treated buccal mucosa squamous cell carcinoma (BMSCC) patients. Materials and Methods: A retrospective study was performed by reviewing the medical records of 409 pathologically proven buccal mucosa cancer patients, who were diagnosed and surgically treated in Tata Memorial Hospital between...

  3. Bacterias celulolíticas aisladas del intestino de termitas (Nasutitermes nigriceps con características probióticas y potencial en la degradación del pasto

    Directory of Open Access Journals (Sweden)

    Cecilia Lara Mantilla

    2013-01-01

    Abstract: The objective of this research was to isolate cellulolytic bacteria from the gut of termites (Nasutitermes nigriceps to determine their probiotic properties in vitro and its potential in degrading  grass. Termites were treated with antibacterial detergent, then their intestine was separated and macerated in sterile peptone water. Dilutions of this mixture were inoculated in Petri dishes using  Luria Bertani (LB method and carboxymethylcellulose (CMC 2%, incubated at 37 ° C for 24 hours, and then revealed with 1% Congo Red. Bacteria with higher degradation halos were subjected to Gram staining and probiotic temperature tests, pH, salinity and the presence of bile salts, as well as antagonism and degradation of grass tests. The results revealed the presence of 9 Gram (-  cellulolytic bacillifrom which the bacilli BTN7 and BTN8, showed the best degradation halosof 12 and 14 mm in diameter respectively, growing suitably in the different probiotic tests with densities between 106 and 108 CFU / ml, the degradation percentages of dry matter was 39.73% and 36.10% within 48 and 72 hours respectively. Biochemical tests API E showed that (bioMérieux SA bacilli BTN8 and BTN7 belong to the genus Enterobacter sp. The above mentioned results open the possibility of using these organisms as additives for ruminants feeding, in order to contribute to a better use of pasture, and other lignocellulosic vegetal substrates. Key words:Probiotics, Maralfalfa (Pennisetum sp., ruminal microbes, lignocellulose, Enterobacter.

  4. Tomographic mapping of the hard palate and overlying mucosa

    Energy Technology Data Exchange (ETDEWEB)

    Marquezan, Mariana; Nojima, Lincoln Issamu; Freitas, Amanda Osorio Ayres de; Baratieri, Carolina; Alves Junior, Matheus; Nojima, Matilde da Cunha Goncalves; Araujo, Monica Tirre de Souza, E-mail: marianamarquezan@gmail.com [Department of Pedodontics and Orthodontics, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, RJ (Brazil)

    2012-01-15

    The aim of this study was to measure the thickness of the hard palate and its overlying mucosa using cone-beam computed tomography (CBCT), for purposes of miniscrew placement. The sample comprised 36 CBCT scans of patients aged 12 to 52 years from a database of the Orthodontics Department of the Federal University of Rio de Janeiro. Paracoronal views of the palatal region were reconstructed at 4, 8, 16 and 24 mm posterior to the incisive foramen. In each reconstruction measurements were taken at the suture, 3 mm and 6 mm bilaterally to it. Wilcoxon's test verified the differences between the selected regions. Total bone height decreased from the anterior to the posterior region. In cross sections 4, 16 and 24, bone height decreased from the suture laterally to the 3 mm region and then increased in the 6 mm region. The cortical thickness does not seem to be a concern because it presented a mean thickness of at least 1 mm at all sites evaluated. The measurements of the mucosa thickness decreased from lateral to median and from anterior to posterior regions. The most suitable areas for miniscrew placement in the palate are located 4 mm posterior to the incisive foramen, in the median or paramedian regions 3 mm adjacent to the suture. (author)

  5. Heterotopic gastric mucosa involving the gallbladder and biliary tree

    Energy Technology Data Exchange (ETDEWEB)

    Madrid, Carmen; Berrocal, Teresa; Gorospe, Luis; Prieto, Consuelo [Department of Paediatric Radiology, Hospital Infantil ' ' La Paz' ' , Paseo de la Castellana 261, 28046 Madrid (Spain); Gamez, Manuel [Department of Paediatric Surgery, Hospital Infantil ' ' La Paz' ' , Madrid (Spain)

    2003-02-01

    A case of heterotopic gastric mucosa in the common bile duct, cystic duct and gallbladder is reported in a 3-year-old girl with abdominal pain and jaundice. Abdominal US and CT showed dilatation of the biliary tree and a well-defined mass in the common bile duct that narrowed its lumen. The gallbladder was contracted in both examinations. The common bile duct and the gallbladder were resected and a choledochojejunostomy was performed. Although gastric heterotopy has been described throughout the entire length of the gastrointestinal tract, it is a very uncommon finding in the gallbladder and extremely rare in the biliary tree. A firm diagnosis of gastric heterotopia is based on the presence of fundal mucosa replete with parietal and chief cells. To our knowledge, this is the fifth reported case of heterotopic gastric tissue within the common bile duct, and the first to describe the US and CT findings. A relevant literature review and brief outline of the histological and radiological features are included in the discussion. (orig.)

  6. Heterotopic gastric mucosa involving the gallbladder and biliary tree

    International Nuclear Information System (INIS)

    Madrid, Carmen; Berrocal, Teresa; Gorospe, Luis; Prieto, Consuelo; Gamez, Manuel

    2003-01-01

    A case of heterotopic gastric mucosa in the common bile duct, cystic duct and gallbladder is reported in a 3-year-old girl with abdominal pain and jaundice. Abdominal US and CT showed dilatation of the biliary tree and a well-defined mass in the common bile duct that narrowed its lumen. The gallbladder was contracted in both examinations. The common bile duct and the gallbladder were resected and a choledochojejunostomy was performed. Although gastric heterotopy has been described throughout the entire length of the gastrointestinal tract, it is a very uncommon finding in the gallbladder and extremely rare in the biliary tree. A firm diagnosis of gastric heterotopia is based on the presence of fundal mucosa replete with parietal and chief cells. To our knowledge, this is the fifth reported case of heterotopic gastric tissue within the common bile duct, and the first to describe the US and CT findings. A relevant literature review and brief outline of the histological and radiological features are included in the discussion. (orig.)

  7. Morphological evaluation of tongue mucosa in burning mouth syndrome.

    Science.gov (United States)

    Sardella, Andrea; Gualerzi, Alice; Lodi, Giovanni; Sforza, Chiarella; Carrassi, Antonio; Donetti, Elena

    2012-01-01

    The aim of the present study was to perform a morphological evaluation by immunofluorescence of biomarkers of keratinocyte intercellular adhesion, and of differentiation in the tongue mucosa of burning mouth syndrome patients (BMS), compared with a control group. A prospective blinded evaluation of tongue mucosal specimens processed for light microscopy was performed. Intercellular adhesion was evaluated by investigating the expression of desmoglein 1, desmoglein 3, and of occludin. Keratin 10 and keratin 14 (markers of epithelial differentiation) were also evaluated, as keratin 16 (marker for activated keratinocytes after epithelial injury). Apoptotic cascade was investigated by p53 and activated caspase-3 expression. The basal membrane integrity was analysed through laminin immunoreactivity. In both groups, a preserved three-dimensional architecture of the tongue was observed. Desmoglein 1 and desmoglein 3 epithelial distributions were similar in the desmosomes of patients and control subjects. Again, keratin 10 immunoreactivity and distribution pattern of keratin 14 in the epithelial compartment was similar in both groups. In control samples, keratin 16 immunoreactivity was scant throughout the epithelium with a punctuate and scattered cytoplasmic labelling. In contrast, in all BMS patients keratinocyte cytoplasm was homogeneously labelled for keratin 16, with a more intense staining than controls. Furthermore, keratin 16 staining progressively decreased proceeding towards the most superficial epithelial layers. The results of this study are consistent with and support the clinically normal features of oral mucosa in BMS, and suggest that keratin 16 may be involved in the cell mechanisms underlying the syndrome occurrence. Copyright © 2011 Elsevier Ltd. All rights reserved.

  8. Tomographic mapping of the hard palate and overlying mucosa

    International Nuclear Information System (INIS)

    Marquezan, Mariana; Nojima, Lincoln Issamu; Freitas, Amanda Osorio Ayres de; Baratieri, Carolina; Alves Junior, Matheus; Nojima, Matilde da Cunha Goncalves; Araujo, Monica Tirre de Souza

    2012-01-01

    The aim of this study was to measure the thickness of the hard palate and its overlying mucosa using cone-beam computed tomography (CBCT), for purposes of miniscrew placement. The sample comprised 36 CBCT scans of patients aged 12 to 52 years from a database of the Orthodontics Department of the Federal University of Rio de Janeiro. Paracoronal views of the palatal region were reconstructed at 4, 8, 16 and 24 mm posterior to the incisive foramen. In each reconstruction measurements were taken at the suture, 3 mm and 6 mm bilaterally to it. Wilcoxon's test verified the differences between the selected regions. Total bone height decreased from the anterior to the posterior region. In cross sections 4, 16 and 24, bone height decreased from the suture laterally to the 3 mm region and then increased in the 6 mm region. The cortical thickness does not seem to be a concern because it presented a mean thickness of at least 1 mm at all sites evaluated. The measurements of the mucosa thickness decreased from lateral to median and from anterior to posterior regions. The most suitable areas for miniscrew placement in the palate are located 4 mm posterior to the incisive foramen, in the median or paramedian regions 3 mm adjacent to the suture. (author)

  9. Canine oral mucosa evaluation as a potential autograft tissue for the treatment of unresponsive keratoconjunctivitis sicca.

    Science.gov (United States)

    Cherry, Rose L; Smith, Jodi D; Ben-Shlomo, Gil

    2018-01-01

    Labial mucosa transplantation for the treatment of canine keratoconjunctivitis sicca (KCS) has been reported recently. Postoperative alleviation of clinical signs was noted and assumed to be the result of labial salivary glands providing lubrication to the ocular tissue. The aim of this study was to evaluate the presence of minor salivary glands (MSG) in the canine oral mucosa. Oral mucosal biopsies were collected from six dogs that died (n = 1) or were euthanized (n = 5) for reasons unrelated to this study. The breeds included were two Doberman Pinschers, one Labrador Retriever, one Portuguese Water Dog, one German Shepherd Dog, and one mixed canine. Three were spayed females, and three were castrated males with the median age of 9 years (range, 6-13 years). Samples were obtained by an 8-mm punch biopsy at the following locations of the canine oral cavity: upper rostral labial mucosa at midline, lower rostral labial mucosa at midline, upper labial mucosa near the commissure, lower labial mucosa near the commissure, and buccal mucosa approximately 1 cm caudal to the commissure. Samples were routinely processed with hematoxylin and eosin, and periodic acid-Schiff stains. Samples were evaluated by light microscopy. At the selected locations, no MSG or other secreting cells were detected. Minor salivary glands are not associated with alleviation of canine KCS symptoms following labial mucosa transplantation. Further studies are needed to determine the mechanism leading to the transient improvement of KCS symptoms in canine patients following labial mucosa transplantation. © 2017 American College of Veterinary Ophthalmologists.

  10. Estudio Histológico del Tracto Digestivo del Neón Cardenal Paracheirodon axelrodi (Characidae)

    OpenAIRE

    Gómez-Ramírez, E; Obando, M. J; Tovar, M. O; Caldas, M. L; Hurtado, H

    2011-01-01

    Paracheirodon axelrodi es el pez ornamental que más se exporta en Colombia. Sin embargo, se desconocen varios aspectos de su biología básica. Se estudió la histología del tracto digestivo. Se sacrificaron 15 ejemplares (MS 222, 0,5 g/L), fijados en formaldehído al 4 % y decalcificados con ácido nítrico 7 %. Se siguió el procedimiento para H&E. El tracto digestivo de P. axelrodi presentó cuatro capas constitutivas: mucosa, submucosa, muscular y serosa. La mucosa de la boca presentó un epitelio...

  11. Peces del Noroeste del Ecuador

    OpenAIRE

    Barriga, Ramiro

    1994-01-01

    La ictiofauna del occidente del Ecuador es poco conocida. Los peces del noroccidente son diferentes a los del suroccidente del Ecuador. 34 familias y 82 especies fueron colectadas que equivale al 11 % de las especies de peces continentales registradas en el Ecuador. Icteogeográficamente se sabe que la costa ecuatoriana posee dos provincias: la del Pacifico Norte y la del Guayas, se determinó que el límite de las dos provincias es el río Santiago ya que las especies del mencionado río so...

  12. Estado de conservación de la prótesis y presencia de lesiones en mucosas de pacientes portadores de prótesis parcial removible

    OpenAIRE

    Bernal Luna, Cinthya del Pilar

    2010-01-01

    Objetivo: El objetivo del presente estudio fue determinar la relación entre el estado de conservación de la prótesis y la presencia de lesiones en mucosas de pacientes portadores de prótesis parcial removible Material y método: Se realizó un estudio descriptivo, transversal y prospectivo. En este estudio se evaluaron 50 pacientes adultos (mayores de 60 años) portadores de prótesis parcial removible. Se realizó el examen clínico odontológico y se registraron los hallazgos en una fi...

  13. Experimental model of traumatic ulcer in the cheek mucosa of rats Modelo experimental de úlcera traumática em mucosa jugal de ratos

    OpenAIRE

    Galyléia Meneses Cavalcante; Renata Janaína Sousa de Paula; Leonardo Peres de Souza; Fabrício Bitu Sousa; Mário Rogério Lima Mota; Ana Paula Negreiros Nunes Alves

    2011-01-01

    PURPOSE: To establish an experimental model of traumatic ulcer in rat cheek mucosa for utilization in future alternative therapy studies. METHODS: A total of 60 adult male rats (250 - 300g) were used. Ulceration of the left cheek mucosa was provoked by abrasion using a nº 15 scalpel blade. The animals were observed for 10 days, during which they were weighed and their ulcers were measured. The histological characteristics were analyzed and scored according to the ulcer phase. In the statistic...

  14. In vitro culturing of porcine tracheal mucosa as an ideal model for investigating the influence of drugs on human respiratory mucosa

    OpenAIRE

    Stennert, Eberhard; Siefer, Oliver; Zheng, Meihua; Walger, Martin; Mickenhagen, Axel

    2008-01-01

    It has been previously shown that fresh mucosa from different mammals could serve as raw material for in vitro culturing with the differentiation of cilia, which are the most important morphological structures for the function of the mucociliary system. Increasing legal restrictions on the removal of human tissue and changing surgical techniques have led to a lack of fresh human mucosa for culturing. Most of the animals that have been used as donors up to now are genetically not very close to...

  15. Synovial Sarcoma of the Buccal Mucosa: A Rare Case Report

    Directory of Open Access Journals (Sweden)

    Kumar T. S. Mahesh

    2013-01-01

    Full Text Available Synovial sarcoma (SS is a rare malignant neoplasm that arises most commonly in joint capsules and articular tendons, but its relationship to the synovium is not always obvious. Synovial sarcoma is a malignant soft tissue tumor representing 5.6% to 10% of all soft tissue sarcomas. They are termed SS because of their histologic resemblance to the synovium, but they rarely involve a synovial structure and are thought to arise from pluripotential mesenchymal cells. The tumor usually occurs in close association with tendon sheaths, bursae, and joint capsules, primarily in the para-articular regions of the extremities, with approximately 9% occurring in the head and neck region. Synovial sarcoma has been reported rarely in the oral cavity. We report a very rare case of Synovial sarcoma of the buccal mucosa in a 24-year-old male patient.

  16. ABO blood group antigens in oral mucosa. What is new?

    DEFF Research Database (Denmark)

    Dabelsteen, Erik

    2002-01-01

    which represent secondary gene products. They are synthesized in a stepwise fashion from a precursor by the action of different glycosyltransferases. In non-keratinized oral mucosa, a sequential elongation of the carbohydrates is associated with differentiation of epithelial cells, resulting...... in expression of precursors on basal cells and A/B antigens on spinous cells. Reduction or complete deletion of A/B antigen expression in oral carcinomas has been reported, a phenotypic change that is correlated with invasive and metastatic potential of the tumours and with the mortality rates of the patients....... Disappearance of the antigens is ascribed to the absence of A or B transferase gene expression. Several studies have shown that loss of A and B antigen expression is associated with increased cell motility, invasion in matrigel, and tumourigenecity in syngenic animals. In vivo studies of human oral wound...

  17. Morphology of mucosa-associated lymphoid tissue in odontocetes.

    Science.gov (United States)

    Silva, Fernanda M O; Guimarães, Juliana P; Vergara-Parente, Jociery E; Carvalho, Vitor L; Carolina, Ana; Meirelles, O; Marmontel, Miriam; Oliveira, Bruno S S P; Santos, Silvanise M; Becegato, Estella Z; Evangelista, Janaina S A M; Miglino, Maria Angelica

    2016-09-01

    This study describes the mucosa-associated lymphoid tissue (MALT) in odontocetes from the Brazilian coast and freshwater systems. Seven species were evaluated and tissue samples were analyzed by light, scanning and transmission electron microscopy, and immunohistochemistry. Laryngeal tonsil was a palpable oval mass located in the larynx, composed of a lymphoepithelial complex. Dense collections of lymphocytes were found in the skin of male fetus and calf. Clusters of lymphoid tissue were found in the uterine cervix of a reproductively active juvenile female and along the pulmonary artery of an adult female. Lymphoid tissues associated with the gastrointestinal tract were characterized by diffusely arranged or organized lymphocytes. The anal tonsil was composed of an aggregate of lymphoid tissue occurring exclusively in the anal canal, being composed of squamous epithelium branches. MALT was present in different tissues and organic systems of cetaceans, providing constant protection against mucosal pathogens present in their environment. © 2016 Wiley Periodicals, Inc.

  18. A dose-surviving fraction curve for mouse colonic mucosa

    International Nuclear Information System (INIS)

    Tucker, S.L.; Thames, H.D. Jr.; Withers, H.R.; Mason, K.A.

    1983-01-01

    A dose-surviving fraction curve representing the response of the mouse colonic mucosa to single doses of 137 Cs gamma radiation was obtained from the results of a multifraction in vivo colony assay. Construction of the curve required an estimated of the average number of clonogens initially present per colonic crypt. The estimated clonogen count (88) was determined by a statistical method based on the use of doses per fraction common to different fractionation protocols. Parameters for the LQ and TC models of cell survival were obtained by weighted least-squares fits to the data. A comparison of the survival characteristics of cells from the mouse colonic and jejunal crypts suggested that the epithelium of the colon is less radiosensitive than that of the jejunum. (author)

  19. Langerhans cells and subsets of lymphocytes in the nasal mucosa

    DEFF Research Database (Denmark)

    Hellquist-Dahl, B; Olsen, K E; Irander, K

    1991-01-01

    Langerhans cells and different lymphocytes were studied in the nasal mucosa of 39 woodwork teachers and a control group of 14 healthy subjects. Ten of the woodwork teachers were sensitized as determined by skin prick test. A panel of different monoclonal antibodies was applied on the frozen nasal...... mucosal specimens. Intraepithelial CD1-positive dendritic cells were found in all specimens. However, there was no difference between the number of these Langerhans cells found in the study group and the number found in the controls. In every specimen the intraepithelial lymphocyte population...... was dominated by T lymphocytes, and there were relatively few B cells. Similarly the ratio between CD4- and CD8-positive lymphocytes in the study group and the controls was the same. In all specimens there was a dominance of T suppressor/cytotoxic cells compared with T helper/inducer cells. The study confirms...

  20. Merkel Cell Carcinoma of the Buccal Mucosa and Lower Lip.

    Science.gov (United States)

    Islam, Mohammed N; Chehal, Hardeep; Smith, Molly Housley; Islam, Sarah; Bhattacharyya, Indraneel

    2018-06-01

    Merkel cell carcinoma (MCC) is an uncommon relatively aggressive neuroendocrine dermal neoplasm first described in 1972 as a tumor of the sun exposed skin. Although most MCC affect the skin of the head and neck, rare primarily oral mucosal cases have been documented. Merkel cells are nondendritic neuroendocrine cells that are found not only in the skin but also the oral mucosa and give rise to MCC. Neuroendocrine cells may be found as aggregates in organs or as diffuse or isolated cells within organs and their epithelial lining. They contain peptide hormones and biogenic amines and occur in two forms: dendritic, which are not associated with nerve fibers and non-dendritic, which are associated with nerve fibers. Merkel cells as well as MCC express simple epithelium-type Cytokeratins (8, 18, 19, 20), neurosecretory substances; chromogranin A, synaptophysin, neuron-specific enolase (NSE), adhesion molecules, and villin (intermediate filament). Though weakly, they also express neural markers such as S-100 protein. Cytokeratin 20, and Cluster of differentiation 56, are the two key diagnostic markers for Merkel cells and MCC. Etiology includes UV radiation, the recently described Merkel cell polyomavirus, and long term systemic immunosuppression. The cutaneous and mucosal variants of MCC are considered aggressive tumors with a high risk for local recurrence and metastasis and should be considered in the differential diagnosis of head and neck mucosal lesions. We present two cases of primary Merkel cell carcinoma, one on the buccal mucosa and the other on the lower lip, and discuss the salient histologic, immunohistochemical and clinical features.

  1. Intestinal mucosa development in broiler chickens fed natural growth promoters

    Directory of Open Access Journals (Sweden)

    ERL Pelicano

    2005-12-01

    Full Text Available This study evaluated the use of probiotics and prebiotics on the histological and morphological indexes of the intestinal mucosa of broilers at 21 days of age. Thirty-six birds were randomly distributed in a 3 x 3 factorial arrangement, considering 3 probiotics and prebiotics sources in the diet. There were 9 treatments with 4 repetitions. Diet treatments were: 1 - Control (without growth promoters; 2 - Bacillus subtilis-based probiotic (Pro 1; 3 - Probiotic (Pool based on Lactobacillus acidophilus and casei, Streptococcus lactis and faecium, Bifidobacterium bifidum and Aspergillus oryzae (Pro 2; 4 - Prebiotic based on Phosphorylated Mannanoligosaccharide (MOS and Organic Acidifier (OA (Pre 1; 5 - MOS-based prebiotic (Pre 2; 6 - Pro 1 + Pre 1; 7 - Pro 1 + Pre 2; 8 - Pro 2 + Pre 1; 9 - Pro 2 + Pre 2. Higher villus height (VH (p<0.01 were seen in the duodenum of birds fed diets without prebiotics, whereas birds fed Bacillus subtilis-based probiotic and birds fed prebiotic based on MOS and OA showed higher VH (p<0.01 in jejunum and ileum. Greater crypt depths (CD (p<0.01 were observed in the duodenum, jejunum and ileum of birds receiving B. subtilis, and in the duodenum and jejunum of birds fed diets without prebiotics. Significant interaction (p<0.01 between the evaluated factors was seen for both, VH and CD, in the three intestinal portions. Greater VH was obtained in duodenum, jejunum and ileum with the use of probiotics and prebiotics and greater CD with the use of probiotics, in relation to the control group. There was no difference in villus density (VD between birds fed diets without additives or diets containing probiotics and prebiotics. Nevertheless, there was a significant interaction (p<0.05 between the evaluated factors for VD in the duodenum. Concluding, beneficial effects were seen in histological indexes of the intestinal mucosa with the use of probiotics and prebiotics at 21 days of age.

  2. A novel method for delineation of oral mucosa for radiotherapy dose–response studies

    International Nuclear Information System (INIS)

    Dean, Jamie A.; Welsh, Liam C.; Gulliford, Sarah L.; Harrington, Kevin J.; Nutting, Christopher M.

    2015-01-01

    There is currently no standard method for delineating the oral mucosa and most attempts are oversimplified. A new method to obtain anatomically accurate contours of the oral mucosa surfaces was developed and applied to 11 patients. This is expected to represent an opportunity for improved toxicity modelling of oral mucositis

  3. A novel mechanism for NETosis provides antimicrobial defense at the oral mucosa

    DEFF Research Database (Denmark)

    Mohanty, Tirthankar; Sjögren, Jonathan; Kahn, Fredrik

    2015-01-01

    Neutrophils are essential for host defense at the oral mucosa and neutropenia or functional neutrophil defects lead to disordered oral homeostasis. We found that neutrophils from the oral mucosa harvested from morning saliva had released neutrophil extracellular traps (undergone NETosis) in vivo...

  4. Effect of soybean-lecithin as an enhancer of buccal mucosa absorption of insulin.

    Science.gov (United States)

    Tian, Weiqun; Hu, Qiaolin; Xu, Ying; Xu, Yi

    2012-01-01

    Transmucosal delivery is a suitable route for insulin non-injection administration. In order to understand how insulin passes through mucosa with soybean-lecithin as an enhancing absorption. The penetration rate of insulin molecular through porcine buccal mucosa was investigated by measuring transbuccal fluxes in the Ussing Chambers. The imaging morphology of rabbits buccal mucosa was analyzed by using non-contact mode atomic force microscopy. The permeation rate can be increased by co-administration of soybean-lecithin. Untreated buccal mucosa showed relatively smooth surface characteristics, with many small crater-like pits and indentations spread over mucosa surfaces. Buccal mucosa that had been treated with 1.0% (w/v) sodium deoxycholic acid (pH 7.4) appeared to much more indentations characteristic, which treated with 2.5% (w/v) soybean-lecithin (pH 7.4) and 2.5% (w/v) Azone or laurocapram (pH 7.4) appeared rather different, the surface mucosa treated with soybean-lecithin emulsion showed a fine, rippling effect whereas those exposed to Azone display a more coarse, undulating surface feature. As a result of that Azone could damage the surface of the buccal mucosa, but soybean-lecithin could not. This study demonstrated that soybean-lecithin is a better and safer enhancer for insulin transmucosal delivery.

  5. Acute mucositis in the stimulated oral mucosa of patients during radiotherapy for head and neck cancer

    International Nuclear Information System (INIS)

    Maciejewski, B.; Zajusz, A.; Pilecki, B.; Swiatnicka, J.; Skladowski, K.; Trott, K.R.

    1991-01-01

    In 16 patients treated for squamous cell carcinoma of the oral cavity or oropharynx with an accelerated split course regimen, acute mucosal reactions were significantly less in the left buccal mucosa which had been repeatedly painted with 2% silver-nitrate solution for several days before radiotherapy than in the unpainted right buccal mucosa. (author). 8 refs., 3 figs., 1 tab

  6. Ex Vivo Correlation of the Permeability of Metoprolol Across Human and Porcine Buccal Mucosa

    DEFF Research Database (Denmark)

    Meng-Lund, Emil; Marxen, Eva; Pedersen, Anne Marie Lynge

    2014-01-01

    .0. In addition, hematoxylin-eosin and Alcian blue-van Gieson were used as tissue stains to evaluate the histology and the presence of acidic polysaccharides (e.g., mucins), respectively. The permeability of metoprolol was decreased in human buccal mucosa by almost twofold when compared with porcine buccal mucosa...

  7. Inicial nasal mucosa detachment using piezoelectric device in the Le Fort I osteotomy: A technical note

    OpenAIRE

    Shinohara, Elio Hitoshi; Kaba, Shajadi Carlos Pardo; Ruiz, Marcelo Martinson; Horikawa, Fernando Kendi

    2013-01-01

    In maxillary Le Fort I type osteotomy the detachment of the nasal mucosa should be done carefully. Piezoelectric surgery contributed much to increase the safety of osteotomies, despite the initial advantage of minimizing the risk of injury in nervous tissue, mainly in bilateral sagittal split osteotomy; we use the piezoelectric device for the initial detachment of the nasal mucosa in the maxillary osteotomy.

  8. [The current situation of occupational protection against skin/mucosa contamination among obstetrician and gynecologist].

    Science.gov (United States)

    Zhang, Yujuan; Li, Peng; Wang, Jianmei; Huo, Yan; Yang, Jing

    2015-03-01

    To investigate the occurrence and protection of skin/mucosa contamination among obstetrician and gynecologist. By random, stratified, and clustered sampling, 219 obstetrician and gynecologist were selected. 210 valid questionnaires were collected, efficiency is 95.89%. 110 obstetrician and gynecologist come from tertiary hospitals, 100 come from secondary hospitals. A self-administered questionnaire on skin/mucosa contamination was employed to gauge the implementation of protection, types and working links of skin/mucosa contamination. Of the respondents, only 14.76% (31/210) and 5.24% (11/210) adhered to proper hand washing and wearing protective glasses within nearly 1 year in practical work. Of the respondents, 73.81% (155/210) had experienced the skin/mucosa contamination during their vocation, 50.95% (107/210) occurred sharps injuries, 45.71% (96/210) occurred damaged skin contamination, and 43.33% (91/210) occurred mucosa contamination. Sharps injuries mainly occurred when abdominal operation (45.71%, 96/120), damaged skin contamination mainly occurred when gynecological examination (21.43%, 45/210), and mucosa contamination mainly occurred when midwifery (37.14%, 78/210). The implementation of protective measures is inadequate and incidence of skin/mucosa contamination is higher among obstetrician and gynecologist. Therefore, occupational protection education should focus on different types and working links of skin/mucosa contamination. At the same time, strict supervision and management system should be established.

  9. Histopathologic study of human vocal fold mucosa unphonated over a decade.

    Science.gov (United States)

    Sato, Kiminori; Umeno, Hirohito; Ono, Takeharu; Nakashima, Tadashi

    2011-12-01

    Mechanotransduction caused by vocal fold vibration could possibly be an important factor in the maintenance of extracellular matrices and layered structure of the human adult vocal fold mucosa as a vibrating tissue after the layered structure has been completed. Vocal fold stellate cells (VFSCs) in the human maculae flavae of the vocal fold mucosa are inferred to be involved in the metabolism of extracellular matrices of the vocal fold mucosa. Maculae flavae are also considered to be an important structure in the growth and development of the human vocal fold mucosa. Tension caused by phonation (vocal fold vibration) is hypothesized to stimulate the VFSCs to accelerate production of extracellular matrices. A human adult vocal fold mucosa unphonated over a decade was investigated histopathologically. Vocal fold mucosa unphonated for 11 years and 2 months of a 64-year-old male with cerebral hemorrhage was investigated by light and electron microscopy. The vocal fold mucosae (including maculae flavae) were atrophic. The vocal fold mucosa did not have a vocal ligament, Reinke's space or a layered structure. The lamina propria appeared as a uniform structure. Morphologically, the VFSCs synthesized fewer extracellular matrices, such as fibrous protein and glycosaminoglycan. Consequently, VFSCs appeared to decrease their level of activity.

  10. Opinión de los médicos españoles sobre el síndrome de intestino irritable: Resultados de un estudio utilizando el método Delphi Spanish physicians´point of view on irritable bowel syndrome (IBS: Results of a Delphi survey

    Directory of Open Access Journals (Sweden)

    C. Almansa

    2007-04-01

    Full Text Available Introducción: el síndrome del intestino irritable (SII es una patología controvertida, donde ante la escasez de evidencia científica las recomendaciones sobre su manejo clínico y su definición vienen determinadas por métodos de consenso de expertos. Objetivo: obtener la opinión de los médicos de nuestro país implicados en el manejo de pacientes con SII y conocer los puntos de acuerdo en las principales cuestiones clínicas que plantea. Material y métodos: se realizó un estudio Delphi a nivel nacional mediante dos envíos a 100 médicos de atención primaria y gastroenterólogos. El cuestionario incluía preguntas sobre el concepto, diagnóstico y tratamiento del SII, evaluando el grado de acuerdo entre los participantes. Resultados: el 55% de los encuestados completó los dos envíos. Se obtuvo acuerdo respecto a los síntomas que caracterizan al SII y los de alarma. No existió una actitud clara ante el paciente sin síntomas de alarma, pero sí acuerdo respecto a la realización de colonoscopia en su presencia. Se señaló la necesidad de realizar estudios complementarios en mayores de 40 años. En el tratamiento no encontramos una actitud uniforme, aunque parece existir una orientación diferente según el síntoma predominante. Conclusiones: los médicos participantes en el estudio demuestran a través de sus respuestas un buen conocimiento conceptual del SII, si bien las discrepancias observadas en las cuestiones sobre diagnóstico y tratamiento demuestran la complejidad y falta de uniformidad que existe en el manejo diario de este síndrome.Introduction: lack of evidence in most clinical situations regarding irritable bowel syndrome (IBS enhances the importance of an expert's opinion, which will guide management and even the concept underlying the disease. Objective: to delve into the knowledge and degree of agreement on main clinical skills for this syndrome among physicians involved in its management. Material and method: two

  11. Síndrome do intestino curto na criança: tratamento com nutrição parenteral domiciliar Short bowel syndrome in children: treatment with home parenteral nutrition

    Directory of Open Access Journals (Sweden)

    Uenis Tannuri

    2004-09-01

    Full Text Available OBJETIVO: Em 1979 o autor utilizou, pela primeira vez no Brasil, a nutrição parenteral prolongada domiciliar em criança. O objetivo deste trabalho é o de apresentar a experiência da utilização deste método no tratamento de crianças com síndrome do intestino curto nos últimos 23 anos. MÉTODOS: Dezenove crianças com esta afecção (ressecção de mais de 75% do comprimento intestinal foram tratadas inicialmente em hospital e a seguir no próprio domicílio. Os períodos totais de terapia nutricional variaram de quatro meses a quatro anos e meio, enquanto que as crianças permaneceram em nutrição parenteral domiciliar por períodos que variaram de uma semana a quatro anos, com mediana de oito meses. As soluções nutrientes completas, contendo aminoácidos, glicose, emulsão lipídica, eletrólitos, vitaminas e micro-elementos foram administradas através de catéteres venosos centrais do tipo Broviac ou Hickman. No domicílio, as soluções foram administradas durante o período diurno ou noturno, segundo a preferência dos familiares. RESULTADOS: Em todos os casos verificou-se ganho ponderal, crescimento e desenvolvimento satisfatórios, semelhantes aos obtidos durante nutrição oral. Obstruções do cateter, alterações hepáticas e infecção devida ao cateter foram as complicações mais freqüentes. Sete crianças (37% sobreviveram e estão fora de tratamento. Doze crianças faleceram (dez com resecção total do intestino delgado, sendo 11 por complicações relacionadas à nutrição parenteral (nove por infecção sistêmica e dois por embolia pulmonar maciça e uma por complicação neurológica, dois meses após transplante duplo de intestino e fígado. CONCLUSÃO: A nutrição parenteral domiciliar em crianças com síndrome do intestino curto traz indiscutíveis benefícios, permite redução do período de internação hospitalar, tornando possível a adaptação funcional do intestino remanescente e manutenção do

  12. In vitro culturing of porcine tracheal mucosa as an ideal model for investigating the influence of drugs on human respiratory mucosa.

    Science.gov (United States)

    Stennert, Eberhard; Siefer, Oliver; Zheng, Meihua; Walger, Martin; Mickenhagen, Axel

    2008-09-01

    It has been previously shown that fresh mucosa from different mammals could serve as raw material for in vitro culturing with the differentiation of cilia, which are the most important morphological structures for the function of the mucociliary system. Increasing legal restrictions on the removal of human tissue and changing surgical techniques have led to a lack of fresh human mucosa for culturing. Most of the animals that have been used as donors up to now are genetically not very close to human beings and must all be sacrificed for such studies. We, therefore, established a modified system of culturing mucosa cells from the trachea of pigs, which is available as a regular by-product after slaughtering. With respect to the possibility of developing "beating" cilia, it could be shown that the speed of cell proliferation until adhesion to the coated culture dishes, the formation of conjunctions of cell clusters and the proliferation of cilia were comparable for porcine and human mucosa. Moreover, it could be demonstrated that the porcine cilia beat frequency of 7.57 +/- 1.39 Hz was comparable to the human mucosa cells beat frequency of 7.3 +/- 1.4 Hz and that this beat frequency was absolutely constant over the investigation time of 360 min. In order to prove whether the reaction to different drugs is comparable between the porcine and human cilia, we initially tested benzalkonium chloride, which is known to be toxic for human cells, followed by naphazoline, which we found in previous studies on human mucosa to be non-toxic. The results clearly showed that the functional and morphological reactions of the porcine ciliated cells to these substances were similar to the reaction we found in the in vitro cultured human mucosa.

  13. Comparative evaluation of eosinophils in normal mucosa, dysplastic mucosa and oral squamous cell carcinoma with hematoxylin-eosin, Congo red, and EMR1 immunohistochemical staining techniques.

    Science.gov (United States)

    Kargahi, Neda; Razavi, Sayyed Mohammad; Deyhimi, Parviz; Homayouni, Solmaz

    2015-01-01

    Oral squamous cell carcinoma is the most common malignant lesion of the oral cavity, and it involves various molecular mechanisms. The development of oral squamous cell carcinoma is influenced by the host immune cells, such as eosinophils. The present study was conducted to compare the presence of eosinophils in normal mucosa, dysplastic mucosa, and oral squamous cell carcinoma by -hematoxylin- eosin staining, Congo red staining, and epidermal growth factor-like (EGF-like) module containing a mucin-like hormone receptor1 (EMR1) immunohistochemical marker. In this cross-sectional study, 60 paraffinized samples were selected, consisting of 20 normal mucosae, 20 dysplastic mucosae, and 20 squamous cell carcinoma samples. After confirmation of the diagnosis, the mean number of eosinophils was evaluated by hematoxylin-eosin, Congo red, and immunohystochemical staining techniques. The data were analyzed by SPSS-10 software using the Kruskal-Wallis and Friedman tests. The results showed that the number of eosinophils in dysplastic mucosa was significantly higher than the number in normal mucosa, and the number of eosinophils in squamous cell carcinoma was significantly higher than the number in dysplastic mucosa in all staining techniques (p<0.001). Moreover, the comparison of staining techniques showed a significantly higher number of eosinophils in EMR1immunohistochemicalmarker than were observed when Congo red and hematoxylin - eosin (H&E) staining techniques were used (p<0.001). It can be argued that eosinophil contributes to the identification of lesions that have a higher potential of malignant transformation. Moreover, eosinophil can be suggested as an indicator in the differentiation of oral lesions in cases with borderline diagnosis and in targeted molecular therapy.

  14. Modelo de suprimento sanguíneo do intestino grosso do tamanduá bandeira (Myrmecophaga tridactyla Model of blood supply to the intestine of Giant anteater (Myrmecophaga tridactyla

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    Ana Lúcia Rezende de Souza

    2010-03-01

    Full Text Available Em seis animais adultos Myrmecophaga tridactyla estudou-se o modelo de suprimento sanguíneo do intestino grosso, que é dependente das artérias mesentérica cranial (AMCr e caudal (AMC. Os espécimes coletados conforme as normas do IBAMA (Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis foram perfundidos com água (40 C, injetados com látex corado, fixados em formol e conservados em solução alcoólica (50%. O mesocólon está disposto na linha sagital mediana e fixa o intestino grosso à parede dorsal do abdome. Derivaram da AMC: a artéria retal cranial, sete a 14 artérias cólicas e uma ou duas artérias ileocólicas, que apresentaram anastomoses de irrigação com a AMCr. A AMC finaliza-se na borda mesocólica das alças intestinais, emitindo ramos cólicos retos a partir das arcadas justacólicas, que penetram na intimidade da musculatura longitudinal. Ao longo do trajeto da AMC foram observadas ilhas arteriais, e a região ileocólica apresentou maior densidade vascular.This research aimed to study the model of large intestine blood supply, which is dependent on the cranial mesenteric artery (AMCr and caudal mesenteric artery (AMC, in six adults of Myrmecophaga tridactyla. The specimens were collected in accordance with the IBAMA standards (Brazilian Institute of the Environment and were perfused with water (40 C, injected with colored latex, fixed in formaldehyde and preserved in alcohol solution (50%. The mesocolon is positioned in the median sagittal line and fixes the large intestine to the dorsal wall of the abdomen. The following is derived from the AMC: the cranial rectal artery, from 7 to 14 colic arteries and one or two ileocolic arteries, which present irrigation anastomosis with AMCr. The AMC finishes at the mesocolic edge of the bowel loop, generating straight colic branches from juxtacolic arcades, which penetrate the intimacy of longitudinal muscles. Arterial islands were observed along the AMC

  15. Bovine alpha-lactalbumin stimulates mucus metabolism in gastric mucosa.

    Science.gov (United States)

    Ushida, Y; Shimokawa, Y; Toida, T; Matsui, H; Takase, M

    2007-02-01

    Bovine alpha-lactalbumin (alpha-LA), a major milk protein, exerts strong gastroprotective activity against rat experimental gastric ulcers induced by ethanol or stress. To elucidate the mechanisms underlying this activity, the influence of alpha-LA on gastric mucus metabolism was investigated in vitro and in vivo. For the in vitro study, RGM1 cells (a rat gastric epithelial cell line) were selected for observation of the direct activity of alpha-LA on gastric mucosal cells and cultured in the presence of either alpha-LA or ovalbumin (OVA), a reference protein showing no gastroprotective activity. Amounts of synthesized and secreted mucin, a major component of mucus, were determined using [3H]glucosamine as a tracer, and prostaglandin E2 (PGE2) levels in the culture medium were determined by RIA. For the in vivo study, the thickness of the mucus gel layer, a protective barrier for gastric mucosa, was evaluated histochemically in rat gastric mucosa. alpha-Lactalbumin (3 mg/mL) significantly stimulated mucin synthesis and secretion in RGM1 cells and also increased PGE2 levels in the culture medium. In contrast, OVA showed no enhancing effects under identical conditions. Neither indomethacin, a cyclo-oxygenase inhibitor, nor AH23848, a prostaglandin EP4 receptor antagonist, affected alpha-LA-induced enhancement of mucin synthesis and secretion. In vivo, oral administration of alpha-LA (300 mg/kg x 3 times/d x 7 d) increased the thickness of the mucus gel layer in rats. These results indicate that alpha-LA fortifies the mucus gel layer by stimulating mucin production and secretion in gastric mucus-producing cells, and that this enhancing effect is independent of endogenous PGE2. Comparison of the efficacy of alpha-LA with OVA suggests that the activities observed in RGM1 cells are closely related to the gastroprotective effects in rat gastric ulcer models. In conclusion, alpha-LA stimulates mucus metabolism, and this action may be responsible for its gastroprotective

  16. Enfermedad Diverticular del Colon

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    Gonzalo López Escobar

    1991-06-01

    Full Text Available

    Los divertículos del colon han sido reconocidos por varios observadores desde hace más de un siglo, pero en su mayor parte se trataba de casos aislados, hoy se la considera como la enfermedad del siglo XX, la de la era moderna y de los países industrializados y de avanzada tecnología (5,18,33.

    Según el diccionario de la Real Academia Española (11, divertículo, del latín, diverticulum, quiere decir desviación de un camino; y desde el punto de vista anatómico, apéndice hueco y terminado en fondo de saco. (Gráfica No. 1.

    Goligher (17 lo define como la “posada al borde del camino, probablemente un lugar, a menudo, de mala reputación”.

    Historia

    Según Hackford (18, el proceso fué descrito brevemente por Littre a comienzos del siglo XVIII; pero se le atribuye a Cruveilhier la primera descripción como proceso patológico en 1849, quien, además, mencionó: “encontramos, no rara vez, en el sigmoide, entre las bandas de fibras musculares longitudinales, una serie de pequeños tumores piriformes oscuros, que están formados por hernias de la mucosa a través de brechas en la capa muscular” (17.

    Fleischman en 1815 hizo la primera observación de la enfermedad y empleó el término divertículo (45.

    Rokitansky en 1.849, habló de una enfermedad adquirida y consideró que su causa consistía en la constipación (45.

    Virchowen 1853 describió la perisigmoiditis (45.

    En 1859 Sidney Jones informó de una fístula colo-vesical debida a diverticulitis (5,45.

    Loomis en 1870 describe una peritonitis como resultante de una diverticulitis (45.

    En 1877 Ball describió la anatomía patológica de la enfermedad y presentó dos casos de fístula colovesical debidas a diverticulitis (9. Cripps en 1.888 popularizó la colostomía de desviación como tratamiento para la fístula colovesical(18...

  17. Telomere shortening in the colonic mucosa of patients with ulcerative colitis.

    Science.gov (United States)

    Kinouchi, Y; Hiwatashi, N; Chida, M; Nagashima, F; Takagi, S; Maekawa, H; Toyota, T

    1998-06-01

    Telomere length in human somatic cells gradually decreases with the number of cell divisions and is regarded as a marker of somatic cell turnover. Mucosal cells of the affected colon show rapid turnover in individuals with active ulcerative colitis (UC). Telomere length was determined by Southern blot analysis of terminal restriction fragments (TRFs) from the colonic mucosa of 17 patients with UC in remission, two of whom showed dysplasia, and 17 control subjects without colitis. For each individual, mean TRF length was compared between rectal mucosa and unaffected cecal mucosa. The mean TRF length of the rectal mucosa was significantly less than that of cecal mucosa in UC patients (7.87 +/- 0.36kb versus 8.77 +/- 0.21 kb; P = 0.0015, Wilcoxon signed rank test), whereas no significant difference was detected in the control subjects. The extent of telomere shortening was 10.6 +/- 3.35% in UC patients, compared with 0.8 +/- 0.64% in noncolitis controls (P = 0.0024, Mann-Whitney U-test). Four UC patients, two of whom had dysplasia, showed telomere shortening of more than 20% in the rectal mucosa. These observations suggest that telomere shortening in the colonic mucosa of individuals with UC may represent the history of mucosal inflammation during disease of long duration, and that it may contribute to aneuploidy in UC.

  18. Resection and Primary Closure of Edematous Glossoepiglottic Mucosa in a Dog Causing Laryngeal Obstruction.

    Science.gov (United States)

    Schabbing, Kevin J; Seaman, Jeffrey A

    An approximately 22 mo old male neutered English bulldog was evaluated for acute onset of dyspnea with suspected brachycephalic obstructive airway syndrome (BOAS). Laryngoscopic exam revealed diffuse, severe edema and static displacement of redundant glossoepiglottic (GE) mucosa causing complete obstruction of the larynx and epiglottic entrapment. Static displacement of the GE mucosa was observed and determined to be the overriding component of dyspnea in this patient with BOAS. Resection and primary closure with two separate, simple continuous sutures of the GE mucosa were performed. Resection and primary closure of the GE mucosa resolved the acute onset of dyspnea in this patient. Surgical correction of the stenotic nares, elongated soft palate, and everted laryngeal saccules were performed under the same anesthetic procedure. Static displacement of the GE mucosa may occur in patients with BOAS. Surgical resection and closure of the GE mucosa resolved this patient's dyspnea and is recommended in airway obstruction. It remains to be determined if primary closure and subsequent tensioning or scar tissue of the GE mucosa results in further complications related to restricted epiglottic movement.

  19. Five surgical maneuvers on nasal mucosa movement in cleft palate repair: A cadaver study.

    Science.gov (United States)

    Nguyen, Dennis C; Patel, Kamlesh B; Parikh, Rajiv P; Skolnick, Gary B; Woo, Albert S

    2016-06-01

    This biomechanical study aims to characterize the nasal mucosa during palatoplasty, thereby describing the soft tissue attachments at different zones and quantifying movement following their release. Palatal nasal mucosa was exposed and divided in the midline in 10 adult cadaver heads. Five consecutive maneuvers were performed: (1) elevation of nasal mucosa off the maxilla, (2) dissection of nasal mucosa from soft palate musculature, (3) separation of nasal mucosa from palatine aponeurosis, (4) release of mucosa at the pterygopalatine junction, and (5) mobilization of vomer flaps. The mucosal movements across the midline at the midpalate (MP) and posterior nasal spine (PNS) following each maneuver were measured. At the MP, maneuvers 1-4 cumulatively provided 3.8 mm (36.9%), 4.9 mm (47.6%), 6.1 mm (59.2%), and 10.3 mm, respectively. Vomer flap (10.5 mm) elevation led to mobility equivalent to that of maneuvers 1-4 (p = 0.72). At the PNS, cumulative measurements after maneuvers 1-4 were 1.3 mm (10%), 2.4 mm (18.6%), 5.7 mm (44.2%), and 12.9 mm. Here, vomer flaps (6.5 mm) provided less movement (p < 0.001). Maneuver 4 yielded the greatest amount of movement of the lateral nasal mucosa at both MP (4.2 mm, 40.8%) and PNS (7.2 mm, 55.8%). At the MP, complete release of the lateral nasal mucosa achieves as much movement as the vomer flap. At the hard-soft palate junction, the maneuvers progressively add to the movement of the lateral nasal mucosa. The most powerful step is release of attachments along the posterior aspect of the medial pterygoid. Published by Elsevier Ltd.

  20. Regeneration of Vocal Fold Mucosa Using Tissue-Engineered Structures with Oral Mucosal Cells

    Science.gov (United States)

    Fukahori, Mioko; Chitose, Shun-ichi; Sato, Kiminori; Sueyoshi, Shintaro; Kurita, Takashi; Umeno, Hirohito; Monden, Yu; Yamakawa, Ryoji

    2016-01-01

    Objectives Scarred vocal folds result in irregular vibrations during phonation due to stiffness of the vocal fold mucosa. To date, a completely satisfactory corrective procedure has yet to be achieved. We hypothesize that a potential treatment option for this disease is to replace scarred vocal folds with organotypic mucosa. The purpose of this study is to regenerate vocal fold mucosa using a tissue-engineered structure with autologous oral mucosal cells. Study Design Animal experiment using eight beagles (including three controls). Methods A 3 mm by 3 mm specimen of canine oral mucosa was surgically excised and divided into epithelial and subepithelial tissues. Epithelial cells and fibroblasts were isolated and cultured separately. The proliferated epithelial cells were co-cultured on oriented collagen gels containing the proliferated fibroblasts for an additional two weeks. The organotypic cultured tissues were transplanted to the mucosa-deficient vocal folds. Two months after transplantation, vocal fold vibrations and morphological characteristics were observed. Results A tissue-engineered vocal fold mucosa, consisting of stratified epithelium and lamina propria, was successfully fabricated to closely resemble the normal layered vocal fold mucosa. Laryngeal stroboscopy revealed regular but slightly small mucosal waves at the transplanted site. Immunohistochemically, stratified epithelium expressed cytokeratin, and the distributed cells in the lamina propria expressed vimentin. Elastic Van Gieson staining revealed a decreased number of elastic fibers in the lamina propria of the transplanted site. Conclusion The fabricated mucosa with autologous oral mucosal cells successfully restored the vocal fold mucosa. This reconstruction technique could offer substantial clinical advantages for treating intractable diseases such as scarring of the vocal folds. PMID:26730600

  1. The bacteriology of the small intestinal mucosa of free-living reindeer

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    Wenche Sørmo

    1994-12-01

    Full Text Available Bacteria in close associaton with the intestinal mucosa are thought to protect the mucosa from pathogenic microorganisms. The pH of the small intestinal mucosa and the viable populations of aerobic and anaerobic bacteria associated with the proximal and distal jejunal mucosa, were measured in four free-living reindeer in winter. The anaerobic bacterial populations were characterized. The median pH of the mucosa of the duodenum was 6.6 (n=4 at point 0.2 m from the pyloric sphincter. The mucosal pH increased along the length of the intestine to 8.3 at 14 m and then decreased to 7.9 at 19.8 m from the pyloric sphincter. Examination by transmission electron microscopy and cultivation techniques failed to reveal any bacteria on the mucosa of the proximal jejunum in two of the animals. In two other reindeer the median anaerobic bacterial densities in the proximal jejunum ranged from 25-2500 cells/g mucosa. The median anaerobic bacterial populations in the distal jejunum ranged from 80 to 20000 bacteria/g mucosa (n=4. The anaerobic population of bacteria in the proximal jejunum was dominated by streptococci and unidentified gram positive rods. Bacteroidaceae, streptococci and unidentified gram positive rods were common in the distal jejunum. The low density and the species diversity of bacteria in the small intestine suggests that these microorganisms are inhibited by components in the natural winter diet of reindeer. Bacteria evidently play a minor role in protection of the mucosa of reindeer in winter.

  2. The burden and management of patients with IBS: results from a survey in Spanish gastroenterologists Manejo clínico de los pacientes con síndrome de intestino irritable: resultados de una encuesta entre gastroenterólogos españoles

    Directory of Open Access Journals (Sweden)

    Cristina Almansa

    2011-11-01

    Full Text Available Backgrounds: irritable bowel syndrome (IBS is a common and complex disorder. Though it is estimated that IBS constitutes an important part of the gastroenterology (GI practice, the burden of this problem in the GI outpatients clinics in Spain is unclear. Aim: the aim of this study is to obtain a "shot" of the burden and management of this syndrome in the daily GI practice. Methods: 508 general gastroenterologists from all over the country were approached and asked to complete a survey that included questions about their daily practice and the definition, diagnosis and treatment of IBS. Results: 55% of the surveyed physicians estimate that IBS constitute between a quarter and a half of all their consultations; and pointed out that most of these patients were referred from primary care. Overall, the Spanish gastroenterologists show an adequate knowledge of the different diagnostic criteria, though they do not always follow the current guidelines. Up to 55% of the physicians always perform a complementary test to support their diagnosis and 40% order a diagnostic test only in older patients (> 50 years or in presence of alarm symptoms. Interestingly, 80% of the gastroenterologists start empirical treatment based on the patients' symptoms. Conclusions: though Spanish gastroenterologists seem to have an adequate theoretical knowledge of the disease, they do not systematically follow current guidelines and tend to manage IBS symptoms based on their own criteria.Introducción: el síndrome de intestino irritable (SII es un trastorno frecuente, aunque la magnitud de este problema y su complejidad en las consultas de digestivo en España no están bien caracterizadas. Objetivo: obtener conocimiento sobre cómo manejan clínicamente el SII los especialistas de aparato digestivo en España. Método: encuesta transversal realizada a 508 especialistas de aparato digestivo de toda España que incluía preguntas generales acerca de su práctica clínica diaria

  3. Grain sorghum dust increases macromolecular efflux from the in situ nasal mucosa.

    Science.gov (United States)

    Gao, X P

    1998-04-01

    The purpose of this study was to determine whether an aqueous extract of grain sorghum dust increases macromolecular efflux from the nasal mucosa in vivo and, if so, whether this response is mediated, in part, by substance P. Suffusion of grain sorghum dust extract on the in situ nasal mucosa of anesthetized hamsters elicits a significant increase in clearance of fluorescein isothiocyanate-labeled dextran (FITC-dextran; mol mass, 70 kDa; P grain sorghum dust elicits neurogenic plasma exudation from the in situ nasal mucosa.

  4. One-stage and two-stage penile buccal mucosa urethroplasty

    Directory of Open Access Journals (Sweden)

    G. Barbagli

    2016-03-01

    Full Text Available The paper provides the reader with the detailed description of current techniques of one-stage and two-stage penile buccal mucosa urethroplasty. The paper provides the reader with the preoperative patient evaluation paying attention to the use of diagnostic tools. The one-stage penile urethroplasty using buccal mucosa graft with the application of glue is preliminary showed and discussed. Two-stage penile urethroplasty is then reported. A detailed description of first-stage urethroplasty according Johanson technique is reported. A second-stage urethroplasty using buccal mucosa graft and glue is presented. Finally postoperative course and follow-up are addressed.

  5. Intestinal alkaline phosphatase in the colonic mucosa of children with inflammatory bowel disease

    Science.gov (United States)

    Molnár, Kriszta; Vannay, Ádám; Szebeni, Beáta; Bánki, Nóra Fanni; Sziksz, Erna; Cseh, Áron; Győrffy, Hajnalka; Lakatos, Péter László; Papp, Mária; Arató, András; Veres, Gábor

    2012-01-01

    AIM: To investigate intestinal alkaline phosphatase (iAP) in the intestinal mucosa of children with inflammatory bowel disease (IBD). METHODS: Colonic biopsy samples were taken from 15 newly diagnosed IBD patients and from 10 healthy controls. In IBD patients, specimens were obtained both from inflamed and non-inflamed areas. The iAP mRNA and protein expression was determined by reverse transcription-polymerase chain reaction and Western blotting analysis, respectively. Tissue localization of iAP and Toll-like receptor (TLR) 4 was investigated by immunofluorescent staining. RESULTS: The iAP protein level in the inflamed mucosa of children with Crohn’s disease (CD) and ulcerative colitis (UC) was significantly decreased when compared with controls (both P < 0.05). Similarly, we found a significantly decreased level of iAP protein in the inflamed mucosa in CD compared with non-inflamed mucosa in CD (P < 0.05). In addition, the iAP protein level in inflamed colonic mucosa in patients with UC was decreased compared with non-inflamed mucosa in patients with CD (P < 0.05). iAP protein levels in the non-inflamed mucosa of patients with CD were similar to controls. iAP mRNA expression in inflamed colonic mucosa of children with CD and UC was not significantly different from that in non-inflamed colonic mucosa with CD. Expression of iAP mRNA in patients with non-inflamed mucosa and in controls were similar. Co-localization of iAP with TLR4 showed intense staining with a dotted-like pattern. iAP was present in the inflamed and non-inflamed mucosa of patients with CD, UC, and in control biopsy specimens, irrespective of whether it was present in the terminal ileum or in the colon. However, the fluorescent signal of TLR4 was more pronounced in the colon compared with the terminal ileum in all groups studied. CONCLUSION: Lower than normal iAP protein levels in inflamed mucosa of IBD patients may indicate a role for iAP in inflammatory lesions in IBD. Based on our results

  6. Mejoría mucosa endoscópica en pacientes con enfermedad inflamatoria intestinal

    OpenAIRE

    Veitia, Guillermo; Pernalete, Beatriz; La Cruz, María Alejandra; Rodríguez, María Josefina; Cachima, Lisbeth; Soto, José; Esquerre, Ivanova; Manuitt, Juan; Pérez, Guillermo

    2014-01-01

    La curación mucosa endoscópica en enfermedad inflamatoria intestinal se asocia con remisión clínica sostenida, reducción de hospitalizaciones y cirugía, pero lograrlo es difícil, según la literatura. Por ello evaluamos la mejoría mucosa endoscópica en nuestros pacientes. Objetivo: Evaluar la mejoría mucosa endoscópica en pacientes con enfermedad inflamatoria intestinal. Métodos: estudio multicéntrico, descriptivo, transversal. Incluyó 24 pacientes entre 17 y 73 años. Para evaluar respuesta a ...

  7. Variation in activity of pepsin extracted from buffalo stomach mucosa

    International Nuclear Information System (INIS)

    Firdous, S.; Javed, A.; Miraj, S.; Ejaz, N.

    2013-01-01

    Pepsin was extracted from the buffalo's mucosa in an acidic medium by incubating at 40 degree C for 48 h and dried in an air blanket at 50 degree C. Conditions for the maximum yield M pepsin were optimized. Changes in pH, temperature and incubation time affect the yield of pepsin, It has been noted that the time of the year in which extractions were made under optimized conditions was an important factor which affected the yield as well as activity of pepsin. Studies showed that maximum yield 11.5% was in February 2009 and minimum 10.3% in May 2009. It was further studied that the activity of the pepsin extracted in February was higher i.e 110 U/mg as compared to the activity of the enzyme extracted during the month of May which was 102.6 U/mg. The purpose of the study was to consider the conditions of the slaughter houses to attain maximum yield of pepsin with maximum activity. (author)

  8. Acute effect of gamma irradiation on the gastric mucosa

    International Nuclear Information System (INIS)

    Dubois, A.; Dorval, E.D.; Rogers, J.E.; O'Connell, L.; Durakovic, A.; Conklin, J.J.

    1984-01-01

    The effect of gamma irradiation on the gastric mucosa has been studied in a primate model by evaluating endoscopically the rate of healing of gastric biopsies. Six male rhesus monkeys were subjected to fiberoptic gastroscopies performed under general anesthesia before and after total body exposure to Cobalt-60 (800 rads). Gastric biopsies were taken 3 hours and 2, 7, and 9 days after irradiation and examined using light microscopy. Gastric biopsies were found to heal in 3 days before irradiation; in contrast, they were still present 7 and 9 days after the biopsies in irradiated animals. Microscopic examination of the biopsies taken outside of the ulcer craters did not demonstrate any significant changes of the gastric surface epithelial cells. These data demonstrate that a gastric ulcer develops at the site of each endoscopic biopsy in irradiated monkeys whereas complete healing is observed in non-irradiated animals. The cause of this observation is unclear but it could be due to radiation induced suppression of the mitotic activity and of the cell renewal of gastric surface epithelial cells

  9. Transcriptional Analyses of Barrett's Metaplasia and Normal Upper GI Mucosae

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    Michael T. Barrett

    2002-01-01

    Full Text Available Over the last two decades, the incidence of esophageal adenocarcinoma (EA has increased dramatically in the US and Western Europe. It has been shown that EAs evolve from premalignant Barrett's esophagus (BE tissue by a process of clonal expansion and evolution. However, the molecular phenotype of the premalignant metaplasia, and its relationship to those of the normal upper gastrointestinal (GI mucosae, including gastric, duodenal, and squamous epithelium of the esophagus, has not been systematically characterized. Therefore, we used oligonucleotide-based microarrays to characterize gene expression profiles in each of these tissues. The similarity of BE to each of the normal tissues was compared using a series of computational approaches. Our analyses included esophageal squamous epithelium, which is present at the same anatomic site and exposed to similar conditions as Barrett's epithelium, duodenum that shares morphologic similarity to Barrett's epithelium, and adjacent gastric epithelium. There was a clear distinction among the expression profiles of gastric, duodenal, and squamous epithelium whereas the BE profiles showed considerable overlap with normal tissues. Furthermore, we identified clusters of genes that are specific to each of the tissues, to the Barrett's metaplastic epithelia, and a cluster of genes that was distinct between squamous and nonsquamous epithelia.

  10. CT appearances of mucosa-associated lymphoid tissue (MALT) lymphoma

    International Nuclear Information System (INIS)

    Kessar, P.; Norton, A.; Rohatiner, A.Z.S.; Lister, T.A.; Reznek, R.H.

    1999-01-01

    Mucosa-associated lymphoid tissue (MALT) lymphoma is a low-grade lymphoma that differs from high-grade non-Hodgkin lymphoma both clinically and histologically. The CT appearances of MALT lymphoma are described. Of 40 patients referred with biopsy-proven MALT lymphoma, only seven had not had gastrectomy or chemotherapy prior to CT examination. The CT scans of these seven cases were analysed for the degree and extent of gastric wall thickening, enlargement of abdominal and extra-abdominal lymph nodes, and presence of extranodal disease. In all patients the stomach was distended with oral contrast medium and scans performed at narrow collimation, after intravenous administration of 20 mg hyoscine butylbromide. In six patients focal thickening of the gastric wall was 1 cm or less. One patient had thickening of over 4 cm. There was no enlargement of abdominal or extra-abdominal lymph nodes or extension to adjacent organs. Thus on CT, at presentation, MALT lymphoma results in minimal gastric wall thickening, unlike high-grade non-Hodgkin lymphoma, which typically causes bulky gastric disease, nodal enlargement and extension into adjacent organs. CT is therefore of limited value in monitoring response to treatment. With disease greater than minimal thickening, transformation to a higher grade should be considered. (orig.)

  11. CT findings of pulmonary mucosa-associated lymphoid tissue lymphoma

    International Nuclear Information System (INIS)

    Zhang Weidong; Guan Yubao; Li Chuanxing; Wu Peihong

    2010-01-01

    Objective: To study the CT findings of pulmonary mucosa-associated lymphoid tissue (MALT) lymphoma. Methods: The CT examinations of 12 patients with pathologically proven pulmonary MALT lymphoma were reviewed retrospectively. Evaluated imaging findings included number, distribution, shape, attenuation and other associated findings of each lesion were evaluated. Results: Thirty-two pulmonary lesions, including consolidations, masses, nodules and lesions with ground glass attenuation, were identified in 12 patients. Multiple lesions were founded in 10 of 12 patients and solitary lesion in 2 patients. Multiple lesions found in one lung in 2 patients, and multiple lesions found in both lungs in 8 patients. Ten cases demonstrated 21 consolidation lesions with air bronchogram, and one of the ten cases demonstrated two lesions with airway dilatation. Three cases demonstrated 5 masses or nodular lesions, 3 of these 5 lesions showed air bronchogram. Two cases demonstrated 6 ground glass attenuation lesions. One case showed mediastinal and hilar lymphadenopathy. Conclusion: Pulmonary MALT lymphoma usually appears as multiple bilateral consolidations, masses, nodules with air bronchogram or lesions with ground- glass attenuation at CT imaging. The imaging findings described above and with an indolent clinical course may suggest the diagnosis of pulmonary MALT lymphoma. (authors)

  12. Long-term alterations of oral mucosa in radiotherapy patients

    International Nuclear Information System (INIS)

    Prott, Franz-Josef; Handschel, Joerg; Micke, Oliver; Sunderkoetter, Cord; Meyer, Ulrich; Piffko, Josef

    2002-01-01

    Purpose: The aim of this investigation was to describe the alterations in oral mucosa after radiotherapy. Methods and Materials: Biopsies were taken from patients before irradiation, at 60 Gy, and 6-12 months after radiotherapy. Histomorphological evaluation of the vessels was performed, and endothelial expression of ICAM-1, VCAM-1, and E-selectin was also evaluated, as well as distribution of LFA-1-, Mac-1-, VLA-4-, RM3/1-, 27E10-, and 25F9-bearing cells in the subepithelial tissue. Results: The expression of ICAM-1 was downregulated after radiotherapy, whereas the percentage of LFA-1- and VLA-4-bearing cells increased. VCAM-1 remained at low levels. The subepithelial infiltration was still dominated by RM3/1-positive macrophages. The number of vessels decreased, while the lumina of the remaining vessels in the deeper connective layer increased. Conclusions: The late effects of radiotherapy are characterized by a decreased number of blood vessels and by significantly different expression patterns of the adhesion molecules studied, and of integrins and macrophage subpopulations compared to the conditions before irradiation and at 60 Gy

  13. Postioplastia en toros cebuínos del departamento de Córdoba, Colombia

    OpenAIRE

    Martínez-Martínez, Mastoby Miguel; Cardona-Álvarez, Tosé Alberto; Pérez-Berrío, Diana Marcela

    2017-01-01

    Resumen La postitis es una patología que afecta a la mucosa parietal y extremo anterior de las vainas conjuntivales del prepucio, con afectación de la porción tegumentaria o sin esta, y que puede incapacitar la protrusión del pene. Dentro de las causas predisponentes en toros están las particularidades morfogenéticas, traumáticas, infecciosas pruriginosas, y en el caso del contexto del presente estudio una de las más frecuentes es la falta de higiene. Los signos más evidentes son: dificultad ...

  14. Aspectos histopatológicos y receptores hormonales en mucosa endometrial de mujeres posmenopáusicas con terapia hormonal

    Directory of Open Access Journals (Sweden)

    Victoria Valer

    2005-03-01

    Full Text Available Objetivos: Determinar si existe correlación directa entre la terapéutica hormonal administrada en mujeres posmenopaúsicas, el comportamiento de los receptores estrogénicos y progesterónicos en la mucosa endometrial y los cuadros histológicos más frecuentes de patologías endometriales secundarias al tratamiento. Material y Métodos: Estudio prospectivo, longitudinal de 40 mujeres posmenopaúsicas entre los 43 y 60 años con síndrome climatérico, administrándoles 2 mg de 17 beta estradiol y 1 mg de acetato de noretisterona por día, en forma continua y por vía oral. Se realizó una biopsia endometrial basal y otra postratamiento al octavo mes. Las muestras fueron procesadas para estudio histopatológico e inmunohistoquímico para receptores estrogénicos y progesterónicos. Resultados: La menarquia se presentó a una edad promedio de 13,2 años y la menopausia fisiológica entre 42 y 52 años, con una edad promedio de 46,4 años. La sintomatología climatérica mejoró notablemente con la terapia hormonal. El estudio histopatológico de mucosa endometrial basal mostró 10% de hiperplasia simple y 90% de atrofia; postratamiento, todos los casos presentaron atrofia. Los receptores se expresaron en la mucosa endometrial basal: RE (+ 12,5%, RP (++ 12,5%; postratamiento RE (+ 2,5%, RE (+++ 2,5% y RP(+ 2,5%. Conclusiones: La terapia en dosis diarias vía oral de 2 mg de 17 beta estradiol y 1 mg de acetato de noretisterona disminuye la sintomatología climatérica y produce atrofia endometrial en las pacientes con hiperplasia previa. Existe disminución estadísticamente significativa entre la primera muestra basal y la del octavo mes de la expresión de receptores estrogénicos y progesterona.

  15. Orthotopic small intestine transplantation in dogs with systemic graft drainage Transplante ortotópico de intestino delgado em cães com drenagem sistêmica do enxerto

    Directory of Open Access Journals (Sweden)

    Flávio Henrique Ferreira Galvão

    2005-09-01

    Full Text Available BACKGROUND: Small intestine transplantation has been accepted worldwide to treat complex cases of intestinal failure. Canine intestinal transplantation model is important in training the surgical technique and to study the complications of this procedure. Systemic graft venous drainage is frequently performed in clinic, although the consequences of this partial meso-caval shunt have not been studied in detail. AIM: To describe the surgical technique and clinical outcome of a canine intestinal transplantation model using mesenteric-caval graft drainage. METHOD: Adult mongrel dogs from University of São Paulo Animal Facility, São Paulo, SP, Brazil, were used as donors and recipients in ten consecutives orthotopic intestinal transplantation with mesenteric-caval venous drainage. Clinical examination and body weight measurement were performed daily in all animals. Necropsy was performed in animals presenting moribund state (lethargic posture, diarrhea and loss of over 35% of body weight to determine cause of death and histological changes. RESULTS: Three recipients died before day 2 from technical complications and were excluded from the experiment. The remaining seven animals developed signs of graft rejection with onset on days 3-4 and died or were sacrificed presenting severe graft rejection between days 7-9. Necropsy and histology of the graft confirmed the diagnosis of severe acute cellular rejection. CONCLUSION: Small intestine transplantation with systemic drainage in dogs courses with analogous and lethal outcome between postoperative day 7 to 9 due to strong graft rejection. This model serves as an excellent pre-clinical model to study the main complications related to this transplantation.RACIONAL: O transplante de intestino delgado é atualmente indicado para tratar casos complexos de falência entérica. Transplante intestinal em cão é importante modelo experimental para treino da técnica cirúrgica e para estudar as complica

  16. Neoadjuvant polychemotherapy in combined treatment for oral cavity mucosa carcinoma patients

    International Nuclear Information System (INIS)

    Protsyk, V.S.; Trembach, A.M.; Korobko, E.V.; Garbar, L.I.

    2007-01-01

    Neoadjuvant polychemotherapy and radiotherapy as a pre surgeon combined treatment was applied to 144 patients with cancer of oral cavity mucosa. That method of treatment permit to obtain maximum destroy of tumoral parenchyma and clinical effect to all patients. (authors)

  17. Heterotopic Gastric Mucosa in the Distal Part of Esophagus in a Teenager: Case Report.

    Science.gov (United States)

    Lupu, Vasile Valeriu; Ignat, Ancuta; Paduraru, Gabriela; Mihaila, Doina; Burlea, Marin; Ciubara, Anamaria

    2015-10-01

    Heterotopic gastric mucosa (HGM) of the esophagus is a congenital anomaly consisting of ectopic gastric mucosa. It may be connected with disorders of the upper gastrointestinal tract, exacerbated by Helicobacter pylori. The diagnosis of HGM is confirmed via endoscopy with biopsy. Histopathology provides the definitive diagnosis by demonstrating gastric mucosa adjacent to normal esophageal mucosa. HGM located in the distal esophagus needs differentiation from Barrett's esophagus. Barrett's esophagus is a well-known premalignant injury for adenocarcinoma of the esophagus. Malignant progression of HGM occurs in a stepwise pattern, following the metaplasia-dysplasia-adenocarcinoma sequence.We present a rare case of a teenage girl with HGM located in the distal esophagus, associated with chronic gastritis and biliary duodenogastric reflux. Endoscopy combined with biopsies is a mandatory method in clinical evaluation of metaplastic and nonmetaplastic changes within HGM of the esophagus.

  18. Blood-group-related carbohydrates are expressed in organotypic cultures of human skin and oral mucosa

    DEFF Research Database (Denmark)

    Grøn, B; Andersson, A; Dabelsteen, Erik

    1999-01-01

    cultures. The organotypic skin and oral mucosa cultures showed a histological differentiation pattern analogous to that of normal skin and buccal mucosa, and a tissue-specific expression of carbohydrate structures and cytokeratins. However, both types of organotypic cultures also expressed markers which...... are normally seen during wound healing, including Lewis y, cytokeratin 16, and cytokeratin 19. We conclude that the organotypic cultures of oral mucosa and skin are suitable models for future studies of the function of cell-surface carbohydrates, although the expression of wound healing markers has to be taken...... the function of cell-surface carbohydrates, we established organotypic cultures of skin and buccal mucosa. In these cultures, keratinocytes are grown at the air-liquid interface on a supporting matrix consisting of homologous fibroblasts embedded in a collagen type I gel. We examined the expression of blood...

  19. Mechanical Barriers Restrict Invasion of Herpes Simplex Virus 1 into Human Oral Mucosa.

    Science.gov (United States)

    Thier, Katharina; Petermann, Philipp; Rahn, Elena; Rothamel, Daniel; Bloch, Wilhelm; Knebel-Mörsdorf, Dagmar

    2017-11-15

    Oral mucosa is one of the main target tissues of the human pathogen herpes simplex virus 1 (HSV-1). How the virus overcomes the protective epithelial barriers and penetrates the tissue to reach its receptors and initiate infection is still unclear. Here, we established an ex vivo infection assay with human oral mucosa that allows viral entry studies in a natural target tissue. The focus was on the susceptibility of keratinocytes in the epithelium and the characterization of cellular receptors that mediate viral entry. Upon ex vivo infection of gingiva or vestibular mucosa, we observed that intact human mucosa samples were protected from viral invasion. In contrast, the basal layer of the oral epithelium was efficiently invaded once the connective tissue and the basement membrane were removed. Later during infection, HSV-1 spread from basal keratinocytes to upper layers, demonstrating the susceptibility of the stratified squamous epithelium to HSV-1. The analysis of potential receptors revealed nectin-1 on most mucosal keratinocytes, whereas herpesvirus entry mediator (HVEM) was found only on a subpopulation of cells, suggesting that nectin-1 acts as primary receptor for HSV-1 in human oral mucosa. To mimic the supposed entry route of HSV-1 via microlesions in vivo , we mechanically wounded the mucosa prior to infection. While we observed a limited number of infected keratinocytes in some wounded mucosa samples, other samples showed no infected cells. Thus, we conclude that mechanical wounding of mucosa is insufficient for the virus to efficiently overcome epithelial barriers and to make entry-mediating receptors accessible. IMPORTANCE To invade the target tissue of its human host during primary infection, herpes simplex virus (HSV) must overcome the epithelial barriers of mucosa, skin, or cornea. For most viruses, the mechanisms underlying the invasion into the target tissues of their host organism are still open. Here, we established an ex vivo infection model of

  20. Buccal Mucosa as A Route for Systemic Drug Delivery: A Review

    OpenAIRE

    Dhaval A. Pate; M. R. Pate; K. R. Pate; N. M. Pate

    2012-01-01

    Within the oral mucosal cavity, the buccal region offers an attractive route of administration for systemic drug delivery. The mucosa has a rich blood supply and it is relatively permeable. It is the objective of this article to review buccal drug delivery by discussing the structure and environment of the oral mucosa and the experimental methods used in assessing buccal drug permeation/absorption. Buccal dosage forms will also be reviewed with an emphasis on bioadhesive polymeric based deliv...

  1. Buccal mucosa as a route for systemic drug delivery: a review.

    Science.gov (United States)

    Shojaei, A H

    1998-01-01

    Within the oral mucosal cavity, the buccal region offers an attractive route of administration for systemic drug delivery. The mucosa has a rich blood supply and it is relatively permeable. It is the objective of this article to review buccal drug delivery by discussing the structure and environment of the oral mucosa and the experimental methods used in assessing buccal drug permeation/absorption. Buccal dosage forms will also be reviewed with an emphasis on bioadhesive polymeric based delivery systems

  2. Inicial nasal mucosa detachment using piezoelectric device in the Le Fort I osteotomy: A technical note

    Science.gov (United States)

    Shinohara, Elio Hitoshi; Kaba, Shajadi Carlos Pardo; Ruiz, Marcelo Martinson; Horikawa, Fernando Kendi

    2013-01-01

    In maxillary Le Fort I type osteotomy the detachment of the nasal mucosa should be done carefully. Piezoelectric surgery contributed much to increase the safety of osteotomies, despite the initial advantage of minimizing the risk of injury in nervous tissue, mainly in bilateral sagittal split osteotomy; we use the piezoelectric device for the initial detachment of the nasal mucosa in the maxillary osteotomy. PMID:23853472

  3. Biological effect of low-dose application beta-radiation on the gingival mucosa of dogs

    International Nuclear Information System (INIS)

    Ippolitov, Yu.A.; Kovtun, N.N.; Timofeev, L.V.

    1999-01-01

    Biological effect of low-dose application beta-radiation on the gingival mucosa of dogs is studied. Obtained data illustrate the interactions between tissues in local exposure of live tissue to beta-radiation and determine the threshold total dose as 400 sGy. Higher doses lead to secondary changes in the gingival mucosa after which the tissue barrier does not recover [ru

  4. Inicial nasal mucosa detachment using piezoelectric device in the Le Fort I osteotomy: A technical note

    Directory of Open Access Journals (Sweden)

    Elio Hitoshi Shinohara

    2013-01-01

    Full Text Available In maxillary Le Fort I type osteotomy the detachment of the nasal mucosa should be done carefully. Piezoelectric surgery contributed much to increase the safety of osteotomies, despite the initial advantage of minimizing the risk of injury in nervous tissue, mainly in bilateral sagittal split osteotomy; we use the piezoelectric device for the initial detachment of the nasal mucosa in the maxillary osteotomy.

  5. The effect of cola consumption on oral mucosa in rats.

    Science.gov (United States)

    Kapicloğlu, S; Baki, A H; Tekelioğlu, Y; Araz, K

    2000-01-01

    Drinks that contain phosphoric acid have been shown to have erosive effects and cola drinks are strongly acidic (pH 2.5). Gingivitis may be caused by dietary acids. Therefore, this study analyses the interaction of Coca Cola consumption and oral mucosal damage. Thirty rats were divided into three groups of 10. The animals received saline (pH 7.0) or HCl acid buffered to pH 2.6 or Coca Cola (pH 2.6) per os with 24-h free access to these solutions. A biopsy was taken from the front of the gingiva and the tongue. Histopathological analysis showed no specific lesion and there were no differences among saline, Coca Cola and HCl groups. Flow cytometric analysis was used to assess proliferative activity. In the HCl acid and Coca Cola groups, cell cycle analysis showed that the effects of Coca Cola and HCl acid in inducing oral mucosal damage are similar. In both Coca Cola [G0/G1, 70.38+/-7.9; S, 28.06+/-10.13; G2/M, 1.62+/-2.80; proliferative index (PI), 28.68+/-7.981 and HCI (G0/G1, 67.7+/-18.9; S, 27.8+/-17.5; G2/M, 4.4+/-3.8; PI, 30.9+/-20.98), the rat cell population G0/G1 and G2/M phases were found to be low (p Coca Cola and HCl acid have similar proliferative and regenerative effects on oral mucosa, and it is possible that their regenerative effects are caused as a result of an irritant effect.

  6. Effects of carprofen on the integrity and barrier function of canine colonic mucosa.

    Science.gov (United States)

    Briere, Catherine A; Hosgood, Giselle; Morgan, Timothy W; Hedlund, Cheryl S; Hicks, Merrin; McConnico, Rebecca S

    2008-02-01

    To measure effects of carprofen on conductance and permeability to mannitol and histologic appearance in canine colonic mucosa. Colonic mucosa from 13 mature mixed-breed dogs. Procedures-Sections of mucosa from the transverse colon and proximal and distal portions of the descending colon were obtained immediately after dogs were euthanized. Sections were mounted in Ussing chambers. Carprofen (400 microg/mL) was added to the bathing solution for treated sections. Conductance was calculated at 15-minute intervals for 240 minutes. Flux of mannitol was calculated for three 1-hour periods. Histologic examination of sections was performed after experiments concluded. Conductance was graphed against time for each chamber, and area under each curve was calculated. Conductance X time, flux of mannitol, and frequency distribution of histologic findings were analyzed for an effect of region and carprofen. Carprofen significantly increased mean conductance X time, compared with values for control (untreated) sections for all regions of colon. Carprofen significantly increased mean flux of mannitol from period 1 to period 2 and from period 2 to period 3 for all regions of colon. Carprofen caused a significant proportion of sections to have severe sloughing of cells and erosions involving >or= 10% of the epithelium, compared with control sections. Carprofen increased in vitro conductance and permeability to mannitol in canine colonic mucosa. Carprofen resulted in sloughing of cells and erosion of the colonic mucosa. These findings suggested that carprofen can compromise the integrity and barrier function of the colonic mucosa of dogs.

  7. Impairment of aminopyrine clearance in aspirin-damaged canine gastric mucosa

    International Nuclear Information System (INIS)

    Miller, T.A.; Henagan, J.M.; Loy, T.M.

    1983-01-01

    Using an in vivo canine chambered stomach preparation, the clearance of [ 14 C]aminopyrine across mucosa when intravenously infused and the back-diffusion of this substance from gastric lumen to mucosa when topically applied to gastric epithelium were evaluated in aspirin-damaged gastric epithelium. In mucosa damaged by either 20 mM or 40 mM aspirin, the recovery of [ 14 C]aminopyrine, when topically mixed with acid (pH . 1.1) perfusate solution, was not significantly different from nondamaged control mucosa. In addition, the degree of ''trapping'' of this substance from back-diffusion was not different in damaged mucosa from that observed in nondamaged epithelium. In contrast, when [ 14 C]aminopyrine was intravenously infused, its clearance was significantly impaired in aspirin-damaged mucosa when compared with control studies, as evidenced by the increased ''trapping'' of this substance in injured epithelium. These findings indicate that movement of aminopyrine from plasma to gastric lumen is impaired in damaged epithelium, making the aminopyrine clearance technique an unreliable method to accurately measure absolute gastric blood flow in this experimental setting

  8. Detection of survivin mRNA in healthy oral mucosa, oral leucoplakia and oral cancer.

    Science.gov (United States)

    Lodi, G; Franchini, R; Bez, C; Sardella, A; Moneghini, L; Pellegrini, C; Bosari, S; Manfredi, M; Vescovi, P; Carrassi, A

    2010-01-01

    Survivin is involved in modulation of cell death and cell division processes. Survivin expression in normal adult tissues has not been fully understood, although it is markedly lower than in cancer, where it is over-expressed. To investigate survivin expression in normal, potentially malignant and cancerous oral mucosa. We measured survivin mRNA levels by real-time RT-PCR in specimens of oral mucosa (15 from normal mucosa, 17 from potentially malignant lesions, 17 from neoplasms). Scores were compared using Kruskal-Wallis test and post hoc according to Conover. Chi-squared test was used for dichotomous data. The median relative levels of survivin mRNA resulted six for normal mucosa, eight for potentially malignant lesions, 13 for cancers: differences among these three groups were statistically significant, as between cancer and potentially malignant lesions. Expression in normal mucosa and potentially lesions group showed no significant difference. Low, but not marginal expression of survivin in normal mucosa is a new finding, and it could be explained with the higher sensibility of our methods. Survivin expression in oral potentially malignant lesions might indicate a progressive deregulation of expression paralleling oncogenesis, particularly during the first stages of process, suggesting a putative predictive role for survivin.

  9. Helicobacter pylori in the gastric mucosa of dead children Helicobacter pylori en la mucosa gástrica de cadáveres de niños

    Directory of Open Access Journals (Sweden)

    John Jairo Duque Alzate

    1999-03-01

    Full Text Available 23 children under the age of 12 years who died violently without receiving any treatment, had their gastric mucosa studied by means of he Warthin-Starry stain and immunohistochemistry in search for Helicobacter pylori. It was found that 60.9% (14 cases were positive; of them 64,3% belonged to a low social class and 35,7% to the middle one. Of the positive cases, 9 had acute gastritis, 1 had chronic gastritis and only 4 had normal gastric mucosa. A clear association between Helicobacter pylory and changes in gastritis was observed. En 23 niños menores de 12 años que murieron en forma violenta sin haber recibido tratamiento, se estudiaron para Helicobacter pylori las mucosas gástricas con las coloraciones de hematoxilina eosina, Warthin Starry e inmunohistoquímica. Se encontró que 14 casos (60,9% fueron positivos para esta bacteria, de los cuales 9 (64,3% pertenecían a un estrato social bajo y 5 (35,7% a uno medio. De los casos positivos para H. pylori, 9 tenían gastritis aguda, 1 gastritis crónica y sólo en 4 la mucosa gástrica era normal. Se observó una clara asociación entre H. pylori y cambios de gastritis.

  10. Reação liquenoide à carbamazepina em mucosa bucal: relato de caso Lichenoid reaction to carbamazepine in the oral mucosa: case report

    Directory of Open Access Journals (Sweden)

    Gabriela Artico

    2011-08-01

    Full Text Available As reações liquenoides por drogas são mais comuns na pele, mas também podem ocorrer na mucosa bucal. Estas lesões são de difícil diagnóstico por causa de sua semelhança clínica com às do líquen plano oral idiopático. O presente artigo relata um caso de reação liquenoide em mucosa bucal, associado ao uso de carbamazepina, no qual o processo de diagnóstico é enfatizadoLichenoid drug reactions are more commom in skin, but they may also occur in the oral mucosa. It is difficult to diagnose these lesions due to their clinical similarity to the idiopathic oral lichen planus lesions. The present article reports a case of lichenoid reaction in oral mucosa associated to the use of carbamazepine, emphasizing the diagnostic process

  11. Alteraciones en el eje intestino-riñón durante la enfermedad renal crónica: causas, consecuencias y propuestas de tratamiento

    Directory of Open Access Journals (Sweden)

    Ivan Armando Osuna-Padilla

    2017-07-01

    Full Text Available La enfermedad renal crónica y el estado urémico se asocian con alteraciones en la permeabilidad intestinal y cambios en la microbiota intestinal, provocando una mayor producción y translocación de toxinas urémicas como sulfato de indoxilo (IS y sulfato de p-cresilo (pCS, detonando una respuesta inflamatoria. El estado inflamatorio y el incremento en concentraciones séricas de IS y pCS se han asociado con una mayor mortalidad, mayor número de eventos cardiovasculares y mayores alteraciones en el metabolismo mineral y óseo. Se han estudiado diversas estrategias nutricionales y farmacológicas para modular la microbiota intestinal y mejorar las alteraciones en la permeabilidad intestinal, entre ellas la suplementación con probióticos, prebióticos y simbióticos, modificaciones en la composición de la dieta y uso de agentes adsorbentes. El objetivo del presente trabajo es realizar una revisión de las causas de las alteraciones intestinales y de la microbiota intestinal en el paciente con enfermedad renal crónica, analizando las consecuencias de dichos cambios y las intervenciones estudiadas hasta la actualidad.

  12. Flora bacteriana aeróbica del tracto digestivo del vampiro común, Desmodus rotundus (Chiroptera: Phyllostomidae

    Directory of Open Access Journals (Sweden)

    Gloriana Chaverri

    2006-09-01

    Full Text Available El objetivo de esta investigación fue determinar la flora bacteriana del vampiro común (Desmodus rotundus primordialmente debido a que los datos al respecto están desactualizados, y además por la gran importancia económica de esta especie de murciélago. Veintiún murciélagos fueron recolectados y su flora bacteriana identificada separadamente a nivel de estómago e intestino. Las bacterias fueron identificadas con el Analytical Profile Index (API, y los resultados analizados con el paquete APILAB. Un total de treinta especies fueron aisladas en 16 hembras y cinco machos. Las especies más comunes fueron Escherichia coli y Staphylococcus aureus, aunque otras especies, como Acinetobacter johnsonii, Enterobacter sakazakii, Staphylococcus chromogenes, S. hyicus y S. xylosus también se aislaron con frecuencia. El número de especies identificadas en el estómago y el intestino fue significativamente diferente, siendo el intestino más diverso. Esto ha sido encontrado anteriormente en otros mamíferos, y se atribuye probablemente a la reducción en acidez. Asimismo, la mayoría de las especies identificadas en este estudio forman parte de la flora bacteriana normal del tracto digestivo de mamíferos, aunque también se encontraron otras bacterias comunes en la piel de mamíferos y en ambientes acuáticos Las bacterias de la piel podrían estar colonizando el estómago y/o intestino del vampiro cuando éste tiene contacto con sus presas, lo que sugiere que el hábito alimentario de esta especie facilita su colonización por microorganismos que no se encontrarían comúnmente en su tracto digestivo. Ya que también se identificaron bacterias comunes en ambientes acuáticos, es probable que D. rotundus consuma agua cuando esté disponible, lo que respalda los resultados de otros investigadores, y sugiere que esta podría ser una fuente adicional de invasión microbiana.Aerobic bacterial flora from the digestive tract of the common vampire bat

  13. A preservação do intestino delgado para transplante: a contribuição da oxigenação hiperbárica Small bowel preservation for transplantation: the contribution of the hyperbaric oxygenation

    Directory of Open Access Journals (Sweden)

    Fernando Augusto Garcia Guimarães

    2008-06-01

    Full Text Available RACIONAL: Não há ainda um método que mantenha adequadamente e por tempo prolongado a qualidade e a função do intestino delgado a ser transplantado, apesar da existência de várias modalidades de preservação. Há necessidade de se desenvolver e aprimorar novas opções técnicas. OBJETIVO: Recuperar os artigos que estudaram a aplicação da oxigenação hiperbárica (OHB como técnica de preservação do intestino delgado destinado para transplante, procurando determinar qual a contribuição do método no prolongamento do tempo e qualidade dessa preservação. MÉTODOS: Utilizou-se a base de dados do Medline e da Scielo consultada nos sites www.pubmed.com e www.bireme.br tendo sido selecionados 58 trabalhos pertinentes. A revisão mostrou que a preservação intestinal está associada ao uso de soluções que fornecem substrato oxidativo para a célula assim como acrescentam substâncias que evitam ou reduzem a formação de espécies reativas de oxigênio. A hipotermia e a oxigenação destas soluções preservadoras é de uso corrente e consensual. Nove trabalhos ativeram-se ao uso específico da OHB. Não há consenso sobre sua utilização em trabalhos experimentais. Contudo os estudos não são comparáveis, pois utilizam metodologias diversas e diferentes tipos de avaliação. Discute-se também a complexidade e os custos da OHB. Apesar disto, há indicações que a OHB pode prevenir o edema da hipotermia, manter a oxigenação tecidual durante a preservação e impedir ou diminuir a formação de radicais livres. CONCLUSÃO: A OHB deve ser avaliada como alternativa exclusiva ou complementar às técnicas de preservação, sendo campo fértil de investigação.INTRODUCTION: In spite of the existence of several modalities of preservation, up to now a method that adequately keeps for a long time the quality and the functioning aspects of the small bowel to be transplanted is lacking. AIM: To gather articles using hyper baric

  14. Existe importância na utilização de exames de fisiologia ano retal no diagnóstico da sindrome do intestino irritável? Is there importance in the use of anorectal physiologic tests in the diagnosis of the irritable bowel syndrome?

    Directory of Open Access Journals (Sweden)

    Maria Auxiliadora Prolungatti Cesar

    2009-09-01

    Full Text Available INTRODUÇÃO: Em alguns pacientes a síndrome do intestino irritável e a constipação funcional se confundem, principalmente quando o sintoma predominante na síndrome do cólon irritável é a constipação. Dentre os vários exames alguns testes fisiológicos ano retais avaliam a função esfincteriana e sensibilidade retal OBJETIVO: Verificar se existem diferenças entre as manometrias anais dos pacientes com constipação funcional e síndrome do intestino irritável. MÉTODO: Trata-se de estudo de 55 manometrias e testes de sensibilidade anais realizadas em pacientes atendidos no ambulatório de Fisiologia Anal do Serviço de Clínica Cirúrgica do Hospital Universitário de Taubaté com diagnóstico de constipação intestinal ou síndrome do intestino irritável no período de janeiro de 2006 a maio de 2007. Todos os pacientes possuíam colonoscopia a normal e foram incluídos nos critérios diagnósticos de Roma II para Constipação Funcional e Síndrome do Intestino Irritável. As manometrias foram realizadas com aparelho ALACER, de perfusão com 8 canais. RESULTADOS: Não foram encontradas diferenças entre as manometrias quanto às pressões de repouso, contração e evacuação, assim como nos valores de sensibilidade retal. Encontramos diferenças quanto à dor abdominal desencadeada nos pacientes com síndrome do intestino irritável no momento do volume máximo tolerável em que 69,2% destes pacientes apresentaram dor abdominal. CONCLUSÃO: Os pacientes com a síndrome do intestino irritável apresentam dor à distensão da ampola retal, que não ocorre nos pacientes constipados, na aferição do volume máximo tolerável, não houve diferença em relação aos outros dados da manometria.INTRODUCTION: In some patients the irritable bowel syndrome and the functional constipation is confuse, mainly when the predominant symptom in the irritable bowel syndrome is the constipation. Amongst some examinations the anal manometry

  15. Flavobacterium psychrophilum y su patología en alevines de Onchorhynchus mykiss del centro piscícola El Ingenio, Huancayo

    Directory of Open Access Journals (Sweden)

    Jorge León

    2008-12-01

    Full Text Available En el presente trabajo se reporta la presencia de Flavobacterium psychrophilum, como agente causante de la patología “enfermedad bacteriana del agua fría” en alevines de Onchorhynchus mykiss “trucha arco iris” del Centro Piscícola El Ingenio, Junín (3250 m de altitud. La lesión macroscópica externa más frecuente fue la ulceración profunda de la región dorsal del pez acompañado de un ennegrecimiento localizado de la piel. Internamente se observó una marcada esplenomegalia, palidez del hígado, riñón y branquias, inflamación del intestino y acumulación de líquido ascítico en el peritoneo. No se detectó hemorragia interna. En Agar Cytophaga Modificado (ACM según Anaker & Ordal (1959 fueron aisladas inicialmente 29 Gram negativas, de las cuales según la caracterización fenotípica y pruebas bioquímicas 9 fueron consideradas como F. psychrophilum. Pruebas de susceptibilidad antibiótica mostraron alta sensibilidad de las cepas a Gentamicina, Ceftazidina, oxitetraciclina, Norfloxacina, furazolidona, Ciprofloxacina y Cefoxitina.

  16. Recurrent small intestine intussusception in a patient with Peutz-Jeghers syndrome Invaginación recurrente del intestino delgado en un paciente con síndrome de Pentz-Jeghers

    OpenAIRE

    Orestis Ioannidis; Styliani Papaemmanouil; George Paraskevas; Anastasios Kotronis; Stavros Chatzopoulos; Athina Konstantara; Nikolaos Papadimitriou; Apostolos Makrantonakis; Emmanouil Kakoutis

    2012-01-01

    Peutz-Jeghers syndrome is a rare hereditary autosomal dominant disease caused by a mutation of the tumor suppressor gene serine/threonine kinase 11 located in chromosome 19p13.3. It is characterized by the presence of extensive mucocutaneous pigmentation, especially of the lips and the occurrence of hamartomatous polyps throughout the gastrointestinal tract. Gastrointestinal hamartomas occur predominantly in the small intestine and can become symptomatic leading usually to intestinal obstruct...

  17. Recurrent small intestine intussusception in a patient with Peutz-Jeghers syndrome Invaginación recurrente del intestino delgado en un paciente con síndrome de Pentz-Jeghers

    Directory of Open Access Journals (Sweden)

    Orestis Ioannidis

    2012-01-01

    Full Text Available Peutz-Jeghers syndrome is a rare hereditary autosomal dominant disease caused by a mutation of the tumor suppressor gene serine/threonine kinase 11 located in chromosome 19p13.3. It is characterized by the presence of extensive mucocutaneous pigmentation, especially of the lips and the occurrence of hamartomatous polyps throughout the gastrointestinal tract. Gastrointestinal hamartomas occur predominantly in the small intestine and can become symptomatic leading usually to intestinal obstruction and abdominal pain. We present a case of recurrent intestinal obstruction caused by small bowel intussusception treated by reduction, enterotomy and polypectomy and followed by intraoperative enteroscopy and endoscopic polypectomy.

  18. Preparo do intestino grosso para a coloscopia: usos, abusos e idéias controversas Bowel preparation for colonoscopy: myths and misconceptions

    Directory of Open Access Journals (Sweden)

    Julio César M Santos Jr

    2010-09-01

    Full Text Available O uso rotineiro do exame coloscópico para avaliação, diagnóstico e procedimentos terapêutico das doenças dos cólons e do reto, bem como para rastreamento e prevenção do câncer colorretal, seja em pessoas jovens portadores de doenças reconhecidamente pré-malignas ou em pessoas acima do 50 anos de idade, tem sido considerado um dos mais bem sucedidos projetos de saúde pública de extensão mundial. A fácil aceitação se deve a três principais fatores: primeiro, à adequação técnica e evolução dos aparelhos e a segurança do exame; segundo, ao desenvolvimento prático das habilidades do examinador e, terceiro, a magnificência da imagem revelando amplo acesso às finas características da mucosa, com critérios abrangentes para o diagnóstico. Nesse contexto, o preparo necessário para o resultado expressivo que se intenta tornou-se a parte pior ou menos tolerável da coloscopia, razão pela qual o que está em discussão atualmente é a necessária busca com o objetivo de se encontrar um método de preparo, rápido, eficiente, barato, agradável e, principalmente seguro. Nos últimos 40 anos, entre as várias fórmulas - mecânicas e farmacológicas - com diferentes associações de drogas laxativas, tem sido possível destacar três produtos que, pelo menos por algum tempo, foram referências mundiais na limpeza dos cólons que antecede a coloscopia. São eles: primeiro, a solução de manitol a 10% - descartado por causa de acidentes explosivos; segundo, as soluções de polietileno glicol (PEG, depreciado por conta da exigência da ingestão oral de grandes volumes, pelos consequentes distúrbios do equilíbrio hidroeletrolítico e pela rejeição por parte do paciente; e, o terceiro, o fosfato de sódio (NaP que poderia parecer ideal, mas vem recebendo críticas veementes por causa de seus efeitos colaterais, mormente os nefrotóxicos. Não está em jogo a eficácia desses três produtos, mas a segurança que deveria

  19. Potencial del Agua del suelo

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    Bustamante Heliodoro

    1986-12-01

    Full Text Available La energía potencial del agua presenta diferencias de un punto del suelo a otro; esas diferencias son las que originan el movimiento del agua de acuerdo a la tendencia universal de la materia en el sentido de moverse de donde la energía potencial es mayor a donde dicha energía es menor. En el suelo el agua en consecuencia se mueve hacia donde su energía decrece hasta lograr su estado de equilibrio. Se desprende entonces que la cantidad de energía potencial absoluta contenida en el agua, no es importante por sí misma, sino por su relación con la energía en diferentes lugares dentro del suelo. El concepto Potencial de agua del suelo es un criterio para esta energía.

  20. Post-mortem 3H-thymidine incorporation in human epidermis and oral mucosa

    International Nuclear Information System (INIS)

    Schellmann, B.

    1981-01-01

    Using the 3H-thymidine labelling method, the authors studied post-mortem incorporation activity in the epidermis and oral mucosa of corpses which were stored with their clothes on under conditions of normal room temperature (+20 0 ) and of cooling (+4 0 C). Samples were taken in the form of skin punches at 2 h or 4 h intervals, respec.. Using histo-autoradiograms, the incorporation of 3H-thymidine in dependence from the time interval between the points of time of death and sampling were determined in situe and given as the ratio of labelled cells of the germinative layer per 100 μm length of basement membrane. A linear drop of post-mortem thymidine incorporation rates in epidermis and oral mucosa was found in human corpse skin correlating with increasing temporal distance from the point of time of death. Incorporation rates in the oral mucosa were markedly higher (by a factor of 3 to 5) than those of the epidermis which agrees well with in vivo conditions. No labelling of cell nuclei, i.e. no synthetic activity of the germinative layer, could be detected in the epidermis 35-40 h after individual death at the latest (in the oral mucosa after 45-50 h). However, clear incorporation activities could be observed in the germinative layer of epidermis and oral mucosa after more than 4 d in the case of storage at +4 0 C. (orig./MG) [de

  1. Progressive replacement of oral mucosa by conjunctiva in osteo-odonto-keratoprosthesis: preliminary observations.

    Science.gov (United States)

    Pecorella, Irene; Maurizio, Taloni; Antonio, Ciardi; Giancarlo, Falcinelli

    2006-02-01

    In a Strampelli osteo-odonto-keratoprosthesis, a patch of oral mucosa is frequently used to cover the ocular surface after implantation of an osteodental lamina into the eye. In many cases, gross modifications in the eye covering become apparent a few years later. The aim of this study was to investigate the histologic findings in the clinically modified ocular surface. Biopsies were performed in 7 patients at the junction between the osteodental acrylic lamina and surrounding modified oral mucosa, during surgery for local plastic reconstruction or positioning of antiglaucoma silicone tubes. Specimens were examined by light microscopy. Six of the 7 clinically modified specimens corresponded microscopically to conjunctiva. Typical oral mucosa could still be observed overlying the osteodental acrylic lamina. The production of local regulatory factors is a possible explanation for the survival of oral mucosa over the osteodental acrylic lamina, whereas their absence in distant areas may have induced the oral mucosa to transdifferentiate into a conjunctival-type lining. Alternatively, conjunctival regrowth from forniceal stem cells should be taken into consideration.

  2. Characterizing the Molecular Pathology of Arrhythmogenic Cardiomyopathy in Patient Buccal Mucosa Cells.

    Science.gov (United States)

    Asimaki, Angeliki; Protonotarios, Alexandros; James, Cynthia A; Chelko, Stephen P; Tichnell, Crystal; Murray, Brittney; Tsatsopoulou, Adalena; Anastasakis, Aris; te Riele, Anneline; Kléber, André G; Judge, Daniel P; Calkins, Hugh; Saffitz, Jeffrey E

    2016-02-01

    Analysis of myocardium has revealed mechanistic insights into arrhythmogenic cardiomyopathy but cardiac samples are difficult to obtain from probands and especially from family members. To identify a potential surrogate tissue, we characterized buccal mucosa cells. Buccal cells from patients, mutation carriers, and controls were immunostained and analyzed in a blinded fashion. In additional studies, buccal cells were grown in vitro and incubated with SB216763. Immunoreactive signals for the desmosomal protein plakoglobin and the major cardiac gap junction protein Cx43 were markedly diminished in buccal mucosa cells from arrhythmogenic cardiomyopathy patients with known desmosomal mutations when compared with controls. Plakoglobin and Cx43 signals were also reduced in most family members who carried disease alleles but showed no evidence of heart disease. Signal for the desmosomal protein plakophilin-1 was reduced in buccal mucosa cells in patients with PKP2 mutations but not in those with mutations in other desmosomal genes. Signal for the desmosomal protein desmoplakin was reduced in buccal mucosa cells from patients with mutations in DSP, DSG2, or DSC2 but not in PKP2 or JUP. Abnormal protein distributions were reversed in cultured cells incubated with SB216763, a small molecule that rescues the disease phenotype in cardiac myocytes. Buccal mucosa cells from arrhythmogenic cardiomyopathy patients exhibit changes in the distribution of cell junction proteins similar to those seen in the heart. These cells may prove useful in future studies of disease mechanisms and drug screens for effective therapies in arrhythmogenic cardiomyopathy. © 2016 American Heart Association, Inc.

  3. Cultivated Oral Mucosa Epithelium in Ocular Surface Reconstruction in Aniridia Patients

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    Dariusz Dobrowolski

    2015-01-01

    Full Text Available Purpose. Efficacy of cultivated oral mucosa epithelial transplantation (COMET procedure in corneal epithelium restoration of aniridia patients. Methods. Study subjects were aniridia patients (13 patients; 17 eyes with irregular, vascular conjunctival pannus involving visual axis who underwent autologous transplantation of cultivated epithelium. For the procedure oral mucosa epithelial cells were obtained from buccal mucosa with further enzymatic treatment. Suspension of single cells was seeded on previously prepared denuded amniotic membrane. Cultures were carried on culture dishes inserts in the presence of the inactivated with Mitomycin C monolayer of 3T3 fibroblasts. Cultures were carried for seven days. Stratified oral mucosa epithelium with its amniotic membrane carrier was transplanted on the surgically denuded corneal surface of aniridia patients with total or subtotal limbal stem cell deficiency. Outcome Measures. Corneal surface, epithelial regularity, and visual acuity improvement were evaluated. Results. At the end of the observation period, 76.4% of the eyes had regular transparent epithelium and 23.5% had developed epithelial defects or central corneal haze; in 88.2% of cases visual acuity had increased. VA range was from HM 0.05 before the surgery to HM up to 0.1 after surgery. Conclusion. Application of cultivated oral mucosa epithelium restores regular epithelium on the corneal surface with moderate improvement in quality of vision.

  4. Ablation of capsaicin sensitive afferent nerves impairs defence but not rapid repair of rat gastric mucosa.

    Science.gov (United States)

    Pabst, M A; Schöninkle, E; Holzer, P

    1993-07-01

    Capsaicin sensitive afferent neurones have previously been reported to play a part in gastric mucosal protection. The aim of this study was to investigate whether these nociceptive neurones strengthen mucosal defence against injury or promote rapid repair of the damaged mucosa, or both. This hypothesis was examined in anaesthetised rats whose stomachs were perfused with ethanol (25 or 50% in saline, wt/wt) for 30 minutes. The gastric mucosa was inspected 0 and 180 minutes after ethanol had been given at the macroscopic, light, and scanning electron microscopic level. Rapid repair of the ethanol injured gastric mucosa (reduction of deep injury, partial re-epithelialisation of the denuded surface) took place in rats anaesthetised with phenobarbital, but not in those anaesthetised with urethane. Afferent nerve ablation as a result of treating rats with a neurotoxic dose of capsaicin before the experiment significantly aggravated ethanol induced damage as shown by an increase in the area and depth of mucosal erosions. Rapid repair of the injured mucosa, however, as seen in rats anesthetised with phenobarbital 180 minutes after ethanol was given, was similar in capsaicin and vehicle pretreated animals. Ablation of capsaicin sensitive afferent neurones was verified by a depletion of calcitonin gene related peptide from the gastric corpus wall. These findings indicate that nociceptive neurones control mechanisms of defence against acute injury but are not required for rapid repair of injured mucosa.

  5. Ex Vivo Produced Oral Mucosa Equivalent by Using the Direct Explant Cell Culture Technique

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    Kamile Öztürk

    2012-09-01

    Full Text Available Objective: The aim of this study is the histological and immunohistochemical evaluation of ex vivo produced oral mucosal equivalents using keratinocytes cultured by direct explant technique.Material and Methods: Oral mucosa tissue samples were obtained from the keratinized gingival tissues of 14 healthy human subjects. Human oral mucosa keratinocytes from an oral mucosa biopsy specimen were dissociated by the explant technique. Once a sufficient population of keratinocytes was reached, they were seeded onto the type IV collagen coated “AlloDerm” and taken for histological and immunohistochemical examinations at 11 days postseeding of the keratinocytes on the cadaveric human dermal matrix.Results: Histopathologically and immunohistochemically, 12 out of 14 successful ex vivo produced oral mucosa equivalents (EVPOME that consisted of a stratified epidermis on a dermal matrix have been developed with keratinocytes cultured by the explant technique.Conclusion: The technical handling involved in the direct explant method at the beginning of the process has fewer steps than the enzymatic method and use of the direct explant technique protocol for culturing of human oral mucosa keratinocyte may be more adequate for EVPOME production.

  6. Effects of two alfalfa preparations with different particle sizes on the gastric mucosa in weanlings: alfalfa chaff versus alfalfa pellets

    OpenAIRE

    Vondran, Sarah; Venner, Monica; Vervuert, Ingrid

    2016-01-01

    Background Feeding alfalfa hay is often recommended for its buffering components, like protein and calcium, to prevent lesions of the gastric mucosa in horses. Until now, there has been no information regarding the influence of alfalfa particle size on the gastric mucosa. The aim of this study was to investigate the effects of feeding two alfalfa preparations with different particle sizes (alfalfa chaff vs alfalfa pellets) in comparison with grass hay on the gastric mucosa in weanling horses....

  7. La mucosa nasal como vía y fuente para la medicina regenerativa Nasal mucosa as pathway and source for regenerative medicine

    Directory of Open Access Journals (Sweden)

    Porfirio Hernández-Ramírez

    2011-09-01

    Full Text Available Se mencionan los pilares fundamentales que sustentan a la medicina regenerativa y se señala que de ellos, sin lugar a dudas, el que más ha avanzado es el representado por las células madre, en particular las adultas, que de manera progresiva se han ido extendiendo en la práctica clínica. Se destaca que recién se ha explorado la mucosa nasal como una vía útil y sencilla para el acceso al organismo de elementos potencialmente útiles en la medicina regenerativa, y también como fuente de células madre con posibilidades de introducción en el área clínica. Se comentan las características fundamentales de la mucosa nasal, se mencionan algunos medicamentos que se han usado a través de la ruta intranasal y se refiere la posibilidad de usar esta vía para la administración de células madre que puedan ejercer sus acciones sobre el sistema nervioso central. Estos datos se complementan con los promisorios resultados que se han obtenido con el trasplante de células procedentes de la mucosa nasal.The fundamental pillars of the regenerative medicine were set forth in this paper. One of the most advanced is undoubtedly the field of stem cells, particularly adult stem cells, which has progressively spread into the clinical practice. It was underlined that the nasal mucosa has been recently explored as a useful simple pathway through which the potentially useful elements of regenerative medicine may have access to the body; and also as a source of stem cells with possibilities of being introduced in the clinical area. Comments were made on the fundamental characteristics of the nasal mucosa; some drugs that have been administered through intranasal route were mentioned together with the possibility of using this pathway for stem cells that might have their impact on the central nervous system. All these data were completed with the promising results of transplantation of stem cells from the nasal mucosa.

  8. Biodistribuição do radiofármaco pertecnetato de sódio (Na99mTcO4) em ratos submetidos a ressecção extensa de intestino delgado

    OpenAIRE

    Chacon, Dâmaso de Araújo; Araújo Filho, Irami; Villarim Neto, Arthur; Rêgo, Amália Cínthia Meneses; Azevedo, Ítalo Medeiros; Bernardo Filho, Mário; Brandão Neto, José; Medeiros, Aldo Cunha

    2007-01-01

    PURPOSE: To evaluate the biodistribution of sodium pertecnetate (Na99mTcO4) in organs and tissues, the morphometry of remnant intestinal mucosa and ponderal evolution in rats subjected to massive resection of the small intestine. METHODS: Twenty-one Wistar rats were randomly divided into three groups of 7 animals each. The short bowel (SB) group was subjected to massive resection of the small intestine; the control group (C) rats were not operated on, and soft intestinal handling was performe...

  9. Expresión de citoquinas de la respuesta Th2 en mucosa intestinal de crías de alpaca

    OpenAIRE

    Burga Cisterna, Cesar Abel

    2016-01-01

    Evalúa la expresión génica de las citoquinas IL-4, IL-5, IL-6, IL-10, TGF- y el factor transcripcional GATA3 en la mucosa intestinal de crías de alpacas clínicamente sanas de 2 a 47 días de edad. Para lo cual se formaron grupos etarios conformados por alpacas de 2 a 8 días (grupo 1), 10 a 21 días (grupo 2) y 26 a 47 días de edad (grupo 3) sin importar sexo, raza y desprovistos de signos clínicos de enfermedad. Se tomó la porción media del yeyuno de cada animal de las cuales se extrajeron el ...

  10. Epidermoid Cyst Arising in the Buccal Mucosa: Case Report and Literature

    Directory of Open Access Journals (Sweden)

    Fábio Wildson Gurgel Costa

    2014-01-01

    Full Text Available Epidermoid cysts are benign subcutaneous lesions, and the large majority of these cysts affect the floor of the mouth; however, the buccal mucosa is not the usual site of occurrence. To date, only 5 articles have been published with 6 cases of epidermoid cysts arising in the buccal mucosa. Therefore, the aim of this study was to describe the clinical, histopathological and immunohistochemical features of a case of an epidermoid cyst located in the buccal mucosa. To our knowledge, this is the first report of an oral epidermoid cyst describing an intense foreign body gigantocellular inflammatory reaction against epithelial keratin component. Although the usual diagnosis for epidermoid cysts is based on histopathological findings, this case report addresses novel information regarding the immunohistochemical pattern which may be found in these lesions.

  11. A Foreign Body Granuloma of the Buccal Mucosa Induced by Honeybee Sting

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    Kazuhiko Yamamoto

    2017-01-01

    Full Text Available A foreign body granuloma of the buccal mucosa induced by honeybee sting was reported. The patient was an 82-year-old female who presented with a submucous mass at the right buccal mucosa. The mass was 20 mm in diameter, elastically firm, partly mobile without pain or tenderness, and covered with almost normal mucosa. MR image did not delineate the lesion clearly. Under clinical diagnosis of a benign tumor, the lesion was excised under local anesthesia. The excised lesion was 14×11×9 mm in size and solid and yellowish in cut surface. Histologically, the lesion consisted of granulomatous tissue with a few narrow, curved, eosinophilic structures compatible with decomposed fragments of a honeybee sting and was diagnosed as a foreign body granuloma, although the patient did not recall being stung.

  12. [Assesing the effect of Lepidium meyenii "Maca" on the gastric mucosa in patients with functional dyspepsia].

    Science.gov (United States)

    Benites Goñi, Harold Eduardo; Cok García, Jaime; Bussalleu Cavero, Alejandra; Bustamente Robles, Katherine Yelenia; Zegarra Chang, Arturo; Bravo, Eduar; Bussalleu Rivera, Alejandro

    2014-01-01

    The present study was designed to determine the histological effect of Lepidium meyenii "Maca" on the gastric mucosa in patients with functional dyspepsia. This study consists of a clinical case series, in which the effect of Maca on the gastric histopathology of 29 Peruvian patients diagnosed with functional dyspepsia was examined. The presence of H. pylori, as well as the degree and depth of the gastric mucosa inflammation was evaluated from biopsies obtained before and after the treatment based solely of Maca 3 grams per day for four weeks. Average values of the degree and depth of mucosal inflammation before and after the treatment were compared showing no statistical difference among the samples. Sixteen patients were infected with H. pylori, and they remained infected after the treatment with Maca. A four week long treatment with Maca does not produce significant changes on gastric mucosa of patients with functional dyspepsia, neither on H. pylori eradication.

  13. Expression of core clock genes in colorectal tumour cells compared with normal mucosa

    DEFF Research Database (Denmark)

    Fonnes, S; Donatsky, A M; Gögenur, I

    2015-01-01

    AIM: Experimental studies have shown that some circadian core clock genes may act as tumour suppressors and have an important role in the response to oncological treatment. This study investigated the evidence regarding modified expression of core clock genes in colorectal cancer and its...... expression of colorectal cancer cells compared with healthy mucosa cells from specimens analysed by real-time or quantitative real-time polymer chain reaction. The expression of the core clock genes Period, Cryptochrome, Bmal1 and Clock in colorectal tumours were compared with healthy mucosa and correlated...... with clinicopathological features and survival. RESULTS: Seventy-four articles were identified and 11 studies were included. Overall, gene expression of Period was significantly decreased in colorectal cancer cells compared with healthy mucosa cells. This tendency was also seen in the gene expression of Clock. Other core...

  14. Outcome of buccal mucosa urethroplasty in the management of urethral strictures.

    Science.gov (United States)

    Cakiroglu, Basri; Sinanoglu, Orhun; Arda, Ersan

    2017-06-30

    The objective of the study is to report the outcome of buccal mucosal urethroplasty. The follow up data of 15 patients undergoing single stage urethroplasty from September 2010 to September 2015 were retropectively reviewed. They received buccal mucosa graft for urethroplasty. The patients were followed for complications and outcome. Mean age was 53.7 ± 13.6 The stricture length ranged from 3 to 6 cm (mean 4.4 ± 0.8). The success rate for buccal mucosa urethroplasty (BMU) was 67.7% at 12th month. Three patients presenting with voiding difficulty in the 3rd month and one in the next 12 months, had urethral restenosis. One patient had fistula formation at 6th month postoperatively. Five patients underwent retreatment procedures such as internal urethrotomy, urethroplasty and/or internal urethrotomy. The buccal mucosa is easy to obtain and handle, therefore BMU can be safely and effectively managed outside high volume institutions.

  15. Benign Lesions in Mucosa Adjacent to Intestinal-Type Sinonasal Adenocarcinoma

    Directory of Open Access Journals (Sweden)

    Blanca Vivanco

    2011-01-01

    Full Text Available Occupational exposure to wood dust is a strong risk factor for the development of intestinal-type sinonasal adenocarcinoma (ITAC; however, knowledge on possible precursor lesions or biomarkers is limited. Fifty-one samples of tumor-adjacent mucosa and 19 control samples of mucosa from the unaffected fossa of ITAC patients were evaluated for histological changes and p53 protein expression. Mild dysplasia was observed in 14%, cuboidal metaplasia in 57%, intestinal metaplasia in 8%, squamous metaplasia in 24%, and cylindrocellular hyperplasia in 53% of cases. P53 immunopositivity was generally weak occurring most frequently in squamous metaplasia. Wood dust etiology did not appear of influence on the histological changes, but p53 showed a tendency for higher positivity. Dysplasia adjacent to tumor was indicative of subsequent development of recurrence. In conclusion, precursor lesions do occur in mucosa adjacent to ITAC. This is clinically important, because it may justify the screening of high-risk individuals such as woodworkers.

  16. Primary Pulmonary Mucosa-Associated Lymphoid Tissue Lymphoma with a Nodular Opacity: Report of a Case.

    Science.gov (United States)

    Yoshino, Naoyuki; Hirata, Tomomi; Takeuchi, Chie; Usuda, Jitsuo; Hosone, Masaru

    2017-01-01

    Herein, we describe our experience in treating a case of primary pulmonary mucosa-associated lymphoid tissue lymphoma detected as a nodular opacity. A 79-year-old man was referred to our hospital. Computed tomography showed a nodular opacity measuring 20 mm in diameter with regular margins in segment 5 of the right middle lobe of the lung. Although the bronchoscopic brush cytology result was class III, the patient was tentatively diagnosed with suspected mucosa-associated lymphoid tissue lymphoma. A thoracoscopic right middle lobectomy was performed. The pathological findings showed nodular proliferation of small to medium-sized, mature-appearing atypical lymphoid cells, lymphoepithelial lesions, and vague follicles suggesting follicular colonization in some areas. The patient was diagnosed with low-grade small B-cell lymphoma and mucosa-associated lymphoid tissue lymphoma. He has remained well to date, 23 months after surgery, without evidence of recurrence.

  17. Segmental dilatation of the ileum covered almost entirely by gastric mucosa: report of a case.

    Science.gov (United States)

    Kobayashi, Tsutomu; Uchida, Nobuyuki; Shiojima, Masayuki; Sasamoto, Hajime; Shimura, Tatsuo; Takahasi, Atsusi; Kuwano, Hiroyuki

    2007-01-01

    A 13-year-old boy was referred to our hospital for investigation of intermittent abdominal colic pain and vomiting. He underwent an emergency laparotomy, which revealed a volvulus and segmental dilatation of the ileum. The dilated intestine was not associated with poor intestinal circulation. Because the dilated ileum did not seem to be the cause of the volvulus, we simply released the volvulus. However, after surgery, the patient still suffered from persistent abdominal pain, further episodes of volvulus, and invagination of the dilated ileum. Thus, we performed a second operation to resect the segmental dilatation of the ileum. Pathological examination revealed that most of the mucosa of the dilated ileum was composed of ectopic gastric mucosa. We postulate that the ectopic gastric mucosa led to the formation of segmental dilatation of the ileum.

  18. [An observation of the effect of sulfur dioxide on rat nasal mucosa].

    Science.gov (United States)

    Lu, Z Q

    1990-01-01

    This paper reports the effect of SO2 on rat nasal mucosa. The rats were forced to inhale SO2 and the effect on the nasal mucosa observed. The rats were divided into four groups. The first group inhaled 10ppm SO2; the second group 20ppm; the third group 40 ppm and the fourth group served as control. The observation lasted for 6 months. It was found that the nasal mucosa in the control group remained columnar ciliated. In the experimental groups, during the early stage (groups I, II) of exposure, there was no significant morphological change in epithelium. Then (groups I, II) the epithelium changed into cuboidal with complete disappearance of cilia, only some short and slender microvilli remained. While in the late stage (group III), the epithelium had transformed into squamous stratified and the amount of mucosal glands reduced.

  19. [Treatment of disbacteriosis of gastroduodenal mucous microflora in mucosa inflammation, ulcer and erosion].

    Science.gov (United States)

    Chernin, V V; Chernivets, V M; Bondarenko, V M; Bazlov, S N

    2011-01-01

    To propose pharmacotherapy of disbacteriosis of gastroduodenal mucous microflora in gastroduodenal inflammation, erosion and ulcer. The study enrolled 30 healthy volunteers, 130 ulcer patients and 36 patients with chronic gastritis (27% of the latter had chronic duodenitis). In addition to general clinical examination, fibrogastroduodenoscopy, we made histological and microbiological examinations of biopsy specimens of the mucosa from different parts of the stomach and duodenum, determined sensitivity of the microflora to antibacterial drugs. We found that recurrent ulcer, chronic gastritis and duodenitis are accompanied with overgrowth of pathogenic microflora in gastric and duodenal mucosa. We developed an effective method of the treatment of gastroduodenal mucosa microflora disbacteriosis in gastroduodenal inflammation, erosion and ulcer including antibacterial, antifungal drugs and probiotics.

  20. Surface ultrastuctures of the human laryngeal mucosa - observation by an newly developed technique of SEM cinematography

    International Nuclear Information System (INIS)

    Ohyama, M.; Ohno, I.; Fujita, T.; Adachi, K.

    1981-01-01

    With the newly-developed techniques of SEM cinematography, surface ultrastructures of the human normal and pathological laryngeal mucosa were demonstrated. The high specialization of the laryngeal mucosa with its marked regional differences stresses the fact that even the squamous epithelium and nonciliated epithelium may play a role of utmost importance. All specimens were obtained after laryngectomy from 10 patients affected by laryngeal cancer which had been treated with or without preoperative irradiation of Lineac in total doses of 3,500-4,500 rad. Special attention was paid to the occurrence of microvilli and microplicae in the normal and pathological mucosa of the larynx, and their morphological and physiological significances were discussed briefly. (Auth.)

  1. Duodenal Bulb Mucosa with Hypertrophic Gastric Oxyntic Heterotopia in Patients with Zollinger Ellison Syndrome

    Science.gov (United States)

    Kohan, Emil; Oh, David; Wang, Hank; Hazany, Salar; Ohning, Gordon; Pisegna, Joseph R.

    2009-01-01

    Objectives. Zollinger-Ellison Syndrome (ZES) results in hypersecretion of gastric acid (via gastrinoma) leading to peptic ulcers, diarrhea, and abdominal pain. We describe the novel discovery of hypertrophic, heterotopic gastric mucosa in the proximal duodenal bulb in patients with ZES, which we hypothesize results in an increased incidence of postbulbar ulcers in patients with ZES (a mechanism previously unreported). We determined the incidence of the novel finding of duodenal gastric oxyntic hypertrophic heterotopia (GOH) in patients with ZES. Methods. Seven patients with ZES were enrolled. The diagnosis of ZES was established by hypergastrinemia, gastric acid hypersecretion, and a positive secretin test or based on biopsy specimens (evaluated via tissue staining). Basal acid output (BAO) and baseline gastrin secretion were determined by established methods. Endoscopic examinations with methylene blue staining and biopsy of the gastric and duodenal mucosa were conducted in all patients every 3–6 months for an average of 5 years. Results. The duodenal mucosa demonstrated hypertrophic GOH in 5 out of 7 patients with ZES and an intact stomach and duodenum. Biopsies from the bowel mucosa demonstrated patchy replacement of surface epithelium by gastric-type epithelium with hypertrophic oxyntic glands in the lamina propria in 5 patients. Two of the patients had no evidence of GOH in the duodenal bulb. Patients with GOH had an average serum gastrin level of 1245 pg/mL and BAO of 2.92 mEq/hr versus 724 pg/mL and 0.8 mEq/hr in patients without GOH. Conclusions. This study demonstrated the presence of duodenal mucosa with GOH in 5 out of 7 patients with ZES and an intact stomach and duodenum. The presence of hypertrophic and heterotopic gastric mucosa is proposed to result from increased gastrin levels and may contribute to the increased incidence of postbulbar ulcers in these patients. PMID:19587828

  2. Duodenal Bulb Mucosa with Hypertrophic Gastric Oxyntic Heterotopia in Patients with Zollinger Ellison Syndrome

    Directory of Open Access Journals (Sweden)

    Emil Kohan

    2009-01-01

    Full Text Available Objectives. Zollinger-Ellison Syndrome (ZES results in hypersecretion of gastric acid (via gastrinoma leading to peptic ulcers, diarrhea, and abdominal pain. We describe the novel discovery of hypertrophic, heterotopic gastric mucosa in the proximal duodenal bulb in patients with ZES, which we hypothesize results in an increased incidence of postbulbar ulcers in patients with ZES (a mechanism previously unreported. We determined the incidence of the novel finding of duodenal gastric oxyntic hypertrophic heterotopia (GOH in patients with ZES. Methods. Seven patients with ZES were enrolled. The diagnosis of ZES was established by hypergastrinemia, gastric acid hypersecretion, and a positive secretin test or based on biopsy specimens (evaluated via tissue staining. Basal acid output (BAO and baseline gastrin secretion were determined by established methods. Endoscopic examinations with methylene blue staining and biopsy of the gastric and duodenal mucosa were conducted in all patients every 3–6 months for an average of 5 years. Results. The duodenal mucosa demonstrated hypertrophic GOH in 5 out of 7 patients with ZES and an intact stomach and duodenum. Biopsies from the bowel mucosa demonstrated patchy replacement of surface epithelium by gastric-type epithelium with hypertrophic oxyntic glands in the lamina propria in 5 patients. Two of the patients had no evidence of GOH in the duodenal bulb. Patients with GOH had an average serum gastrin level of 1245 pg/mL and BAO of 2.92 mEq/hr versus 724 pg/mL and 0.8 mEq/hr in patients without GOH. Conclusions. This study demonstrated the presence of duodenal mucosa with GOH in 5 out of 7 patients with ZES and an intact stomach and duodenum. The presence of hypertrophic and heterotopic gastric mucosa is proposed to result from increased gastrin levels and may contribute to the increased incidence of postbulbar ulcers in these patients.

  3. Mycobacterium leprae is identified in the oral mucosa from paucibacillary and multibacillary leprosy patients.

    Science.gov (United States)

    Morgado de Abreu, M A M; Roselino, A M; Enokihara, M; Nonogaki, S; Prestes-Carneiro, L E; Weckx, L L M; Alchorne, M M A

    2014-01-01

    In leprosy, the nasal mucosa is considered as the principal route of transmission for the bacillus Mycobacterium leprae. The objective of this study was to identify M. leprae in the oral mucosa of 50 untreated leprosy patients, including 21 paucibacillary (PB) and 29 multibacillary (MB) patients, using immunohistochemistry (IHC), with antibodies against bacillus Calmette-Guérin (BCG) and phenolic glycolipid antigen-1 (PGL-1), and polymerase chain reaction (PCR), with MntH-specific primers for M. leprae, and to compare the results. The material was represented by 163 paraffin blocks containing biopsy samples obtained from clinically normal sites (including the tongue, buccal mucosa and soft palate) and visible lesions anywhere in the oral mucosa. All patients and 158 available samples were included for IHC study. Among the 161 available samples for PCR, 110 had viable DNA. There was viable DNA in at least one area of the oral mucosa for 47 patients. M. leprae was detected in 70% and 78% of patients using IHC and PCR, respectively, and in 94% of the patients by at least one of the two diagnostic methods. There were no differences in detection of M. leprae between MB and PB patients. Similar results were obtained using anti-BCG and anti-PGL-1 antibodies, and immunoreactivity occurred predominantly on free-living bacteria on the epithelial surface, with a predilection for the tongue. Conversely, there was no area of predilection according to the PCR results. M. leprae is present in the oral mucosa at a high frequency, implicating this site as a potential means of leprosy transmission. © 2013 The Authors Clinical Microbiology and Infection © 2013 European Society of Clinical Microbiology and Infectious Diseases.

  4. Sensory dysfunction of bladder mucosa and bladder oversensitivity in a rat model of metabolic syndrome.

    Directory of Open Access Journals (Sweden)

    Wei-Chia Lee

    Full Text Available PURPOSE: To study the role of sensory dysfunction of bladder mucosa in bladder oversensitivity of rats with metabolic syndrome. MATERIALS AND METHODS: Female Wistar rats were fed a fructose-rich diet (60% or a normal diet for 3 months. Based on cystometry, the fructose-fed rats (FFRs were divided into a group with normal detrusor function or detrusor overactivity (DO. Acidic adenosine triphosphate (ATP solution (5mM, pH 3.3 was used to elicit reflex micturition. Cystometric parameters were evaluated before and after drug administration. Functional proteins of the bladder mucosa were assessed by western blotting. RESULTS: Compared to the controls, intravesical acidic ATP solution instillation induced a significant increase in provoked phasic contractions in both FFR groups and a significant decrease in the mean functional bladder capacity of group DO. Pretreatment with capsaicin for C-fiber desentization, intravesical liposome for mucosal protection, or intravenous pyridoxal 5-phosphate 6-azophenyl-2',4'-disulfonic acid for antagonized purinergic receptors can interfere with the urodynamic effects of intravesical ATP in FFRs and controls. Over-expression of TRPV1, P2X(3, and iNOS proteins, and down-regulation of eNOS proteins were observed in the bladder mucosa of both fructose-fed groups. CONCLUSIONS: Alterations of sensory receptors and enzymes in the bladder mucosa, including over-expression of TRPV1, P2X(3, and iNOS proteins, can precipitate the emergence of bladder phasic contractions and oversensitivity through the activation of C-afferents during acidic ATP solution stimulation in FFRs. The down-regulation of eNOS protein in the bladder mucosa of FFRs may lead to a failure to suppress bladder oversensitivity and phasic contractions. Sensory dysfunction of bladder mucosa and DO causing by metabolic syndrome are easier to elicit bladder oversensitivity to certain urothelium stimuli.

  5. [Reconstruction of oral mucosa with a micro-vascularized fascia-cutaneous flap from the forearm].

    Science.gov (United States)

    Burgueño García, Miguel; Cebrián Carretero, José Luis; Muñoz Caro, Jesús Manuel; Arias Gallo, Javier

    2002-01-01

    Epidermoid carcinoma of jugal mucosa is an aggressive tumor. Its treatment is based on broad excision and reconstruction in order to avoid fibrosis and restriction of mouth opening. Neck dissection and radiotherapy are indicated in selected cases. We display our experience with microvascularized flaps with the aim of preventing the flaws. We reconsider 8 patients (representing 10 flaps) handle in our Department. Besides we discuss other therapeutic alternatives after the growth's removal. The conclusion reached is that the mucovascularized forearm flaps give a great quantity of thin tissue and therefore so results to be the best option for the reconstruction of the jugal mucosa.

  6. Multiple Mucous Retention Cysts (Mucocele of the Oral Mucosa: A Case Report

    Directory of Open Access Journals (Sweden)

    Gholamreza Jahanshahi

    2007-01-01

    Full Text Available To our knowledge, the occurrence of multiple mucoceles is not very common. This case report presents a 62-year-old man with multiple nodules on the upper and lower labial mucosa as well as both buccal mucosae with unknown history. Histopathology evaluation showed minor salivary gland ducts dilated to the point of cyst formation. The cysts seemed to be formed either as a result of dilatation of salivary ducts due to altered secretion or because of an acquired or congenital weakness in the ductal structure. The physiopathology of these findings is discussed.

  7. Oral mucocele of unusual size on the buccal mucosa: clinical presentation and surgical approach.

    Science.gov (United States)

    Seo, Juliana; Bruno, Ingrid; Artico, Gabriela; Vechio, Aluana Dal; Migliari, Dante A

    2012-01-01

    Oral mucoceles are small-size, benign minor salivary gland pathologies. The most frequent localizations of these lesions are the lower lip mucosa. However, in some cases, they grow to an unusual size and hinder the preliminary diagnosis of mucocele. The purpose of this article is to report a case of a large oral mucocele with a diameter of 3.5 cm on the buccal mucosa of a 43-years-old male patient. The surgical procedure was carried out for a complete removal of the lesion.

  8. An evaluation of the effects of epidermal growth factor on irradiation lip mucosa damage in mice

    International Nuclear Information System (INIS)

    Feng Yan

    1994-01-01

    The effect of epidermal growth factor (EGF) on lip mucosa damage by irradiation was explored in mice. EGF was administered in doses of 100 μg/kg/day using different schedules. Mucosal damage was assessed. The metaphase arrest method with vinblastine was used to evaluate the diurnal rhythm of mitosis. EGF in regimens employed did not protect the mouse lip epithelial cells from irradiation induced damage, but it has a demonstrable stimulatory effect on cell proliferation in lip mucosa which is dependent on the schedules of administration. The reasons and mechanisms are discussed

  9. Congestion of mastoid mucosa and influence on middle ear pressure - Effect of retroauricular injection of adrenaline.

    Science.gov (United States)

    Fooken Jensen, Pernille Vita; Gaihede, Michael

    2016-10-01

    Micro-CT scanning of temporal bones has revealed numerous retroauricular microchannels, which connect the outer bone surface directly to the underlying mastoid air cells. Their structure and dimensions have suggested a separate vascular supply to the mastoid mucosa, which may play a role in middle ear (ME) pressure regulation. This role may be accomplished by changes in the mucosa congestion resulting in volumetric changes, which ultimately affect the pressure of the enclosed ME gas pocket (Boyle's law). Further, such mucosa congestion may be susceptible to α-adrenergic stimulation similar to the mucosa of the nose. The purpose of our study was to investigate these hypotheses by recording the ME pressure in response to adrenergic stimulation administered by retroauricular injections at the surface of the microchannels. In a group of 20 healthy adults we measured the ME pressure by tympanometry initially in the sitting position, and then in the supine position over a 5 min period with 30 s intervals. In each subject, the study included 1) a control reference experiment with no intervention, 2) a control experiment with subcutaneously retroauricular injection of 1 ml isotonic NaCl solution, and 3) a test experiment with subcutaneously retroauricular injection of 1 ml NaCl-adrenaline solution. In both control experiments the ME pressure displayed an immediate increase in response to changing body position; this pressure increase remained stable for the entire period up to five minutes. In the test experiments the ME pressure also showed an initial pressure increase, but it was followed by a distinct significant pressure decrease with a maximum after 90 s. The test group was injected with both a 5 and 10% adrenaline solution, but the responses appeared similar for the two concentrations. Subcutaneous retroauricular injection of adrenaline caused a significant pressure decrease in ME pressure compared with control ears. This may be explained by the microchannels

  10. El sistema inmunológico de las mucosas

    Directory of Open Access Journals (Sweden)

    Miriam Zaldívar Ochoa

    2002-10-01

    Full Text Available El sistema inmunológico es muy importante porque protege al organismo de agentes microbianos patógenos, toxinas, partículas extrañas, células tumorales y procesos autoinmunes. Para mantener el equilibrio biológico se necesita que este sistema funcione normalmente, de manera que constituya una fuerte barrera defensiva contra la invasión de agentes nocivos; de no suceder esto, se presentará una inmunodeficiencia de expresión clínica variable. La esencia de la función del sistema inmune es su gran capacidad para la discriminación a escala molecular entre lo propio y lo ajeno, o sea, lo que pertenece al organismo y lo que no, conservando así su individualidad.The immunological system is very important because it protects the organism from pathogenic microbial agents, toxins, foreign particles, tumoral cells and autoimmune processes. To maintain the biological balance, this system should work normally, acting as a strong defensive barrier against the invasion of harmful agents. Otherwise, there will be an immunodeficiency of variable clinical expression. The essence of the function of the immune system is its great capacity for distinguishing its own from the strange at the molecular level, that is, to recognize a difference between what belongs or not to the organism, keeping this way its individuality.

  11. del alcoholismo

    Directory of Open Access Journals (Sweden)

    Rodrigo Arias Duque

    2005-01-01

    Full Text Available Desde el punto de vista farmacológico, es importante comprender qué es el alcohol y cómo actúa en el organismo. No existe una causa simple, sino una interacción complicada de factores neuroquímicos, fisiológicos, psicológicos y sociales que originan y desarrollan esta grave enfermedad fármaco-dependiente. La acción psicofisiológica y farmacodinámica del alcohol es fundamentalmente depresiva, por la reducción de la transmisión sináptica en el sistema nervioso humano. Es un hecho conocido que el consumo excesivo de alcohol causa una disfunción aguda y crónica del cerebro, produciendo trastornos en el sistema nervioso central, presentando alteraciones en la memoria y en las funciones intelectuales como cálculo, comprensión y aprendizaje. A nivel hepático tiene lugar, en su mayoría, el metabolismo del alcohol, produciéndose un hígado graso alcohólico, aumentando el tamaño, terminando en necrosis e inflamación grave del hígado; esto se llama hepatitis alcohólica, y si se sigue consumiendo alcohol se desarrollará la cirrosis. El alcohol también ha estado relacionado con alteraciones del miocardio; se ha constatado en animales de experimentación sanos que tanto la velocidad de contracción del músculo cardiaco y su máxima tensión disminuyen en presencia del alcohol, como consecuencia, la fuerza de cada contracción y el aumento de presión en el ventrículo izquierdo son menores, perdiendo eficacia el corazón como bomba.

  12. Experimental model of traumatic ulcer in the cheek mucosa of rats Modelo experimental de úlcera traumática em mucosa jugal de ratos

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    Galyléia Meneses Cavalcante

    2011-06-01

    Full Text Available PURPOSE: To establish an experimental model of traumatic ulcer in rat cheek mucosa for utilization in future alternative therapy studies. METHODS: A total of 60 adult male rats (250 - 300g were used. Ulceration of the left cheek mucosa was provoked by abrasion using a nº 15 scalpel blade. The animals were observed for 10 days, during which they were weighed and their ulcers were measured. The histological characteristics were analyzed and scored according to the ulcer phase. In the statistical analysis, a value of pOBJETIVO: Estabelecer um modelo experimental de úlcera traumática em mucosa jugal de ratos para utilização em futuros testes de terapias alternativas. MÉTODOS: Foram utilizados 60 ratos, adultos, machos, pesando entre 250 a 300g. A ulceração na mucosa jugal esquerda foi provocada por meio da abrasão desta com uma lâmina de bisturi número 15. Os animais foram observados por um período de 10 dias, sendo estes pesados e suas escoriações mensuradas. As características histológicas foram analisadas e descritas adotando escores para comparação dos estágios da fase da úlcera. Na análise estatística um valor de p<0,01 foi considerado uma resposta estatisticamente significante em todos os casos. RESULTADOS: Durante os cinco primeiros dias os animais perderam peso ("t" Student - p<0,01. A área da úlcera regrediu linearmente com o tempo, estando quase que completamente cicatrizada ao final de dez dias (ANOVA, pós-teste de Tendência - p<0,0001. Os grupos do 1º, 2º e 3º dias tiveram comportamento semelhante havendo uma diminuição dos escores a partir do 4º dia. CONCLUSÃO: O modelo de úlcera na mucosa jugal de ratos proposto pode ser considerado eficaz, apresentando reprodutibilidade confiável e baixo custo.

  13. [The methods of modern reflexotherapy in the combined treatment of patients with erosive-ulcerative processes of the oral mucosa].

    Science.gov (United States)

    Maksimovskaia, L N; Barashkov, G N; Trestsov, N G

    1991-01-01

    The authors suggest a scheme of multiple-modality treatment of patients with erosive ulcerative processes in the buccal mucosa. This scheme was employed in the treatment of such patients and it was conducive to a sooner epithelialization of erosions and ulcers in the buccal mucosa; its analgesic effect was fairly high.

  14. Capacidad antioxidante de la sangre de grado (Croton palanostigma sobre la mucosa gástrica, en animales de experimentación

    Directory of Open Access Journals (Sweden)

    Miguel Sandoval

    2006-09-01

    Full Text Available Introducción: El uso de la sangre de grado para el tratamiento de la gastritis y úlceras gástricas se realiza de manera empírica. En investigaciones anteriores hemos observado su capacidad cicatrizante y protectora de la mucosa gástrica, por la estimulación de la secreción de moco, secreción del jugo gástrico sin alterar el pH e incremento de la actividad péptica; por sus componentes, podría además tener capacidad antioxidante, lo que potenciaría su actividad citoprotectora. Objetivo: Determinar la capacidad antioxidante de la sangre de grado (Croton palanostigma sobre la mucosa gástrica en animales de experimentación, por la prueba de TBARS. Diseño: Estudio prospectivo, analítico-experimental y longitudinal. Lugar: Centro de Investigación de Bioquímica y Nutrición Facultad de Medicina UNMSM. Material biológico: Cuarenta ratas albinas machos, entre 220 a 250 g de peso, distribuidas en 4 grupos. Intervenciones: Previo ayuno sólido de 24 h, se administró a las ratas por canulación orogástrica, en dosis única, del modo siguiente: Grupo basal: glucosa 37,5 kcal/kg de peso. Grupo injuria: alcohol 37,7 kcal/kg en glucosa. Grupo control: alcohol 37,7 kcal/kg con piroxicam 10 mg/kg en glucosa. Grupo experimental: alcohol 37,7 kcal/kg con sangre de grado 0,8 mL/kg, en solución de glucosa. Los animales tuvieron agua para consumo ad libitum. Seis horas después, bajo anestesia con éter vaporizado, se practicó una cirugía abdominal, se extrajo el estómago y se obtuvo la porción glandular, se separó una porción representativa de aproximadamente 0,5 g. El tejido gástrico fue homogenizado con buffer fosfato de sodio 50 mmol/L, a pH 7,4. Se determinó la lipoperoxidación midiendo sustancias reactivas al ácido tiobarbitùrico (TBARS, por método espectrofotométrico a 535 nm y se expresó en nmol/gramo de tejido gástrico. Principales medidas de resultados: Capacidad antioxidante de la sangre de grado (Croton palanostigma

  15. The effects of Strongylus vulgaris parasitism on eosinophil distribution and accumulation in equine large intestinal mucosa.

    Science.gov (United States)

    Rötting, A K; Freeman, D E; Constable, P D; Moore, R M; Eurell, J C; Wallig, M A; Hubert, J D

    2008-06-01

    Eosinophilic granulocytes have been associated with parasite or immune-mediated diseases, but their functions in other disease processes remain unclear. Cause and timing of eosinophil migration into the equine gastrointestinal mucosa are also unknown. To determine the effects of intestinal parasitism on eosinophils in equine large intestinal mucosa. Large intestinal mucosal samples were collected from horses and ponies (n = 16) from the general veterinary hospital population, ponies (n = 3) raised in a parasite-free environment, ponies experimentally infected with 500 infective Strongylus vulgaris larvae and treated with a proprietary anthelmintic drug (n = 14), and a similar group of ponies (n = 7) that received no anthelmintic treatment. Total eosinophil counts and eosinophil distribution in the mucosa were determined by histological examination. A mixed model analysis was performed and appropriate Bonferroni adjusted P values used for each family of comparisons. Pvulgaris and those raised in a parasite-free environment. Experimental infection with S. vulgaris, with or without subsequent anthelmintic treatment, did not change eosinophil counts, and counts were similar to those for horses from the general population. Migration of eosinophils to the equine large intestinal mucosa appears to be independent of exposure to parasites. Large intestinal mucosal eosinophils may have more functions in addition to their role in defence against parasites.

  16. EFFECT OF POLYMER PROSTHETIC CONSTRUCTION TREATED WITH GLOW-DISCHARGE PLASMA ON ORAL MUCOSA (CLINICAL CASE

    Directory of Open Access Journals (Sweden)

    E. O. Kudasova

    2017-01-01

    Full Text Available Prosthetic material should induce minimal pathological reaction when in contact with the oral mucosa, especially during oral rehabilitation of patients with tumors of the oral mucosa. The article presents monitoring of a patient after surgery for cancer of the buccal mucosa at the stage of orthopedic rehabilitation. Treatment plan proposed complex oral rehabilitation. A maxillary complete removable denture was manufactured as well as metalloceramic and partial mandibular dentures. Plasma chemical modification of the removable dentures was planned after correction of the prosthetics and primary adaptation. Removable dentures were applied at the maxilla and mandibula. Per our recommendations, the patient was examined weekly for a month and monthly for 6 months. No new tumors or pathological elements were observed.Conclusions. Changes in critical surface tension of the removable dentures made of acrylic plastic improve their biological characteristics as demonstrated by positive clinical dynamics. Prosthetic constructions with hydrophilic surface are recommended in complex clinical cases when patients with tumors of the oral mucosa require dentures.

  17. PENFIGOIDE DE MEMBRANAS MUCOSAS: A PROPÓSITO DE DOS CASOS CLÍNICOS

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    Beatriz E Casnati Guberna

    Full Text Available El penfigoide de membranas mucosas representa un heterogéneo grupo de enfermedades ampollares, autoinmunes y crónicas, que afecta las mucosas y /o la piel. Es una patología que origina como secuelas, cicatrices que motivan importantes incapacidades funcionales en las mucosas involucradas, provocando ceguera, dificultades en la masticación, la deglución o en la respiración. Su diagnóstico, tratamiento y control deben ser realizados por un equipo multidisciplinario. Se presentan dos casos clínicos de penfigoide de membranas mucosas de localización oral, por considerarlos de interés, dadas sus manifestaciones clínicas y la diferente evolución en cada uno de ellos. Se describen los métodos de diagnóstico y tratamientos durante 4 años de seguimiento y control, realizados en la Cátedra de Semiología y Patología BMF, Clínica de Estomatología de la Facultad de Odontología. Universidad de la República

  18. Oral mucosa and lung cancer: Are genetic changes in the oral ...

    African Journals Online (AJOL)

    2016-02-03

    Feb 3, 2016 ... to lung cancer, although other risk factors (such as genetic tendency) ... analysis of oral mucosa identifying individuals predisposed to lung cancer. ... of the study is that oral epithelial cells of smokers who have lung cancer are ... Stratec Molecular, Berlin, Germany). p53 codon 72 ..... Validity and reliability of.

  19. Histamine stimulates chloride secretion in omeprazole-inhibited frog gastric mucosa

    International Nuclear Information System (INIS)

    McGreevy, J.; Barton, R.; Housinger, T.

    1986-01-01

    Omeprazole (OME) stops hydrogen ion (H) secretion in the histamine (HIST)-stimulated gastric mucosa while the chloride (Cl) which had accompanied the H continues to be pumped into the lumen. This finding suggests that the Cl pump is independent of the H/K ATP-ase driven H pump. To test this hypothesis, 16 Ussing-chambered frog mucosas were exposed to OME prior to HIST stimulation. If the Cl pump is independent, HIST should stimulate Cl secretion in the OME-inhibited mucosa. A 1 hr control (CON) interval preceded exposure to OME (10 -4 M) in the nutrient solution. Potential difference (PD), short-circuit current (Isc), resistance (R), H flux (J/sup H/) and Cl flux (J/sup Cl/ with 36 Cl) were measured every 15 min. After 1 hr of OME exposure, HIST (10 -5 M) was added to the nutrient solution. The findings demonstrate that HIST stimulates Cl secretion in the OME-inhibited bullfrog gastric mucosa

  20. Transparency-enhancing technology allows three-dimensional assessment of gastrointestinal mucosa: A porcine model.

    Science.gov (United States)

    Mizutani, Hiroya; Ono, Satoshi; Ushiku, Tetsuo; Kudo, Yotaro; Ikemura, Masako; Kageyama, Natsuko; Yamamichi, Nobutake; Fujishiro, Mitsuhiro; Someya, Takao; Fukayama, Masashi; Koike, Kazuhiko; Onodera, Hiroshi

    2018-02-01

    Although high-resolution three-dimensional imaging of endoscopically resected gastrointestinal specimens can help elucidating morphological features of gastrointestinal mucosa or tumor, there are no established methods to achieve this without breaking specimens apart. We evaluated the utility of transparency-enhancing technology for three-dimensional assessment of gastrointestinal mucosa in porcine models. Esophagus, stomach, and colon mucosa samples obtained from a sacrificed swine were formalin-fixed and paraffin-embedded, and subsequently deparaffinized for analysis. The samples were fluorescently stained, optically cleared using transparency-enhancing technology: ilLUmination of Cleared organs to IDentify target molecules method (LUCID), and visualized using laser scanning microscopy. After observation, all specimens were paraffin-embedded again and evaluated by conventional histopathological assessment to measure the impact of transparency-enhancing procedures. As a result, microscopic observation revealed horizontal section views of mucosa at deeper levels and enabled the three-dimensional image reconstruction of glandular and vascular structures. Besides, paraffin-embedded specimens after transparency-enhancing procedures were all assessed appropriately by conventional histopathological staining. These results suggest that transparency-enhancing technology may be feasible for clinical application and enable the three-dimensional structural analysis of endoscopic resected specimen non-destructively. Although there remain many limitations or problems to be solved, this promising technology might represent a novel histopathological method for evaluating gastrointestinal cancers. © 2018 Japanese Society of Pathology and John Wiley & Sons Australia, Ltd.

  1. Detection of Helicobacter pylori in the Gastric Mucosa by Fluorescence In Vivo Hybridization

    DEFF Research Database (Denmark)

    Fontenete, Silvia; Leite, Marina; Figueiredo, Céu

    2017-01-01

    In this chapter, we describe a fluorescence in vivo hybridization (FIVH) protocol, using nucleic acid probes, for the detection of the bacterium Helicobacter pylori in the gastric mucosa of an infected C57BL/6 mouse model. This protocol should be easily extended to other microorganisms not only...

  2. Mucosa protectives: sucralfate and colloidal bismuth subcitrate in peptic ulcer disease

    NARCIS (Netherlands)

    Tytgat, G. N.; Nio, C. Y.

    1987-01-01

    Mucosa protective drugs are thought to have an important role in the treatment of both duodenal (DU) and gastric ulcer (GU) disease by means of correcting the disturbed defensive factors. Sucralfate as well as colloidal bismuth subcitrate (CBS) form a layer on the ulcer base and in this way protect

  3. Pathohistologic characteristics of gastric and duodenal mucosa in liquidators of Chernobyl accident with peptic duodenal ulcer

    International Nuclear Information System (INIS)

    Degtyar'ova, L.V.

    2000-01-01

    Pathomorphological characteristics of gastric and duodenal mucosa associated with the dose of ionizing radiation at peptic duodenal ulcer in participants of the Chernobyl accident clean-up was determined. Our findings suggest that the doses of external irradiation exceeding 25 cGy (together with the other harmful effects of the Chernobyl accident) represent a danger of helicobacter infection development

  4. Treatment of Mucosa-associated Lymphoid Tissue Lymphoma in Sjogren's Syndrome : A Retrospective Clinical Study

    NARCIS (Netherlands)

    Pollard, Rodney P. E.; Pijpe, Justin; Bootsma, Hendrika; Spijkervet, Fred K. L.; Kluin, Philip M.; Roodenburg, Jan L. N.; Kallenberg, Cees G. M.; Vissink, Arjan; van Imhoff, Gustaaf W.

    2011-01-01

    Objective. To retrospectively analyze the clinical course of patients with mucosa-associated lymphoid tissue (MALT)-type lymphoma of the parotid gland and associated Sjogren's syndrome (SS). Methods. All consecutive patients with SS and MALT lymphoma (MALT-SS) diagnosed in the University Medical

  5. An endoscopic evaluation of the effects of etodolac and diclofenac on the gastric and duodenal mucosa

    NARCIS (Netherlands)

    van Eeden, A.; Schotborgh, R. H.; Tytgat, G. N.

    1990-01-01

    The effects of two nonsteroidal anti-inflammatory drugs on the gastroduodenal mucosa were evaluated by endoscopy and direct photography in 36 healthy men who were randomly assigned to receive 150 mg of diclofenac, 1,200 mg of etodolac, or 600 mg of etodolac daily for seven days. Endoscopy was

  6. Cytological and histological changes of the rectal mucosa cduring radiotherapy for cervical cancer

    International Nuclear Information System (INIS)

    Sano, Toshiyuki

    1978-01-01

    In a study undertaken to investigate acute radiogenic changes in the rectal mucosa occurring during Linac radiotherapy for carcinoma of the uterine cervix, rectal scrapings and mucosal tissue specimens were obtained under direct vision through a proctoscope and examined cytologically and histologically. The severity of changes in epithelial cells was proportional to the radiation dose. Thus, i) irradiation at a dose of 1000 rads was associated with a slight disorder in the arrangement of epithelial cells as well as enlargement and increased stainability of their nuclei; these changes agreed well with histological findings, ii) at 2000 rads a moderate disorder in the arrangement of epithelial cells, enlargement of nuclei and pyknosis became noticeable; decreased stainability of nuclei and vacuolization were prominent, iii) at 3000 rads disordered arrangement of epithelial cells, enlargement and decreased stainability of nuclei or pyknosis were still more marked and desquamation of epithelial cells, inequality in size and stainability of nuclei and enlargement and increase in the number of nucleoli became prominent. Morphological evidence indicated that the radiosensitivity of the mucosal epithelium of the rectum is lower than that of the small intestine but higher than that of the squamous epithelium of the uterine cervix. After introduction of a central shield there was rapid recovery of the rectal mucosa both cytologically and histologically, thus demonstrating that at dose levels up to 3000 rads no permanent damage occurs in the rectal mucosa. This finding suggests the importance of a central shield in lessening radiation damage to the rectal mucosa. (author)

  7. Survival, Intestinal Mucosa Adhesion, and Immunomodulatory Potential of Lactobacillus plantarum Strains.

    Science.gov (United States)

    Santarmaki, Valentini; Kourkoutas, Yiannis; Zoumpopoulou, Georgia; Mavrogonatou, Eleni; Kiourtzidis, Mikis; Chorianopoulos, Nikos; Tassou, Chrysoula; Tsakalidou, Effie; Simopoulos, Constantinos; Ypsilantis, Petros

    2017-09-01

    Survival during transit through the gastrointestinal track, intestinal mucosa adhesion, and a potential immunomodulatory effect of Lactobacillus plantarum strains 2035 and ACA-DC 2640 were investigated in a rat model. According to microbiological and multiplex PCR analysis, both strains were detected in feces 24 h after either single-dose or daily administration for 7 days. Intestinal mucosa adhesion of L. plantarum 2035 was noted in the large intestine at 24 h after single-dose administration, while it was not detected at 48 h. Daily dosing, prolonged detection of the strain up to 48 h post-administration, and expanded adhesion to the small intestine. Adhesion of L. plantarum ACA-DC 2640 to the intestinal mucosa after single-dose administration was prolonged and more extended compared to L. plantarum 2035. Daily dosing increased both the levels and the rate of positive cultures of the strains compared to those of the single-dose scheme. In addition, both strains increased total IgG while decreased IgM and IgA serum levels. In conclusion, L. plantarum 2035 and L. plantarum ACA-DC 2640 survived transit through the gastrointestinal track, exhibited transient distinct adhesion to the intestinal mucosa and modulated the systemic immune response.

  8. Morphometric study of uninvolved rectal mucosa 10 cm and 20 cm away from the malignant tumor.

    Science.gov (United States)

    Despotović, Sanja Z; Milićević, Novica M; Milosević, Dragoslav P; Despotović, Nebojsa; Erceg, Predrag; Bojić, Bozidar; Bojić, Danijela; Svorcan, Petar; Mihajlović, Gordana; Dorđević, Jelena; Lalić, Ivana M; Milićević, Zivana

    2014-02-01

    Recently, many details of the interplay between tumor cells and tumor-associated stromal elements leading to the progression of malignant disease were elucidated. In contrast, little is known about the role of uninvolved stromal tissue in the remote surrounding of the malignant tumor. Therefore, we performed a computer-aided morphometric study of rectal mucosa in samples taken 10 cm and 20 cm away from the malignant tumor during endoscopic examination of 23 patients older than 60 years. The samples of rectal mucosa from 10 healthy persons of corresponding age subjected to diagnostic rectoscopy during active screening for asymptomatic cancer were used as control. All structural elements of the rectal mucosa were studied and the number of nucleated cells in the lamina propria per 0.1 mm² of tissue was assessed. Our study revealed a reduced number of cells in the lamina propria of the rectal mucosa 10 cm and 20 cm away from the tumor lesion in both male and female patients. The decreased mucosal height and increased crypt number were registered in female patients 10 cm away from the tumor. The connective tissue of lamina propria showed a disorderly organization: the collagen fibers were frail, loosely arranged and signs of tissue edema were present. Small blood vessels and capillaries were much more frequently seen than in healthy tissue. Our results demonstrate the complex interactions between the cancer and remote mucosal tissue of the affected organ.

  9. Characterisation of Roseomonas mucosa isolated from the root canal of an infected tooth.

    Science.gov (United States)

    Diesendorf, Nina; Köhler, Stefanie; Geißdörfer, Walter; Grobecker-Karl, Tanja; Karl, Matthias; Burkovski, Andreas

    2017-06-14

    The genus Roseomonas comprises a group of pink-pigmented, slow-growing, aerobic, non-fermentative Gram-negative bacteria, which have been isolated from environmental sources such as water and soil, but are also associated with human infections. In the study presented here, Roseomonas mucosa was identified for the first time as part of the endodontic microbiota of an infected root canal and characterised in respect to growth, antibiotic susceptibility and biofilm formation. The isolated R. mucosa strain showed strong slime formation and was resistant to most β-lactam antibiotics, while it was susceptible to aminoglycosides, carbapenemes, fluorochinolones, polymyxines, sulfonamides and tetracyclines. Biofilm formation on artificial surfaces (glass, polystyrene, gutta-percha) and on teeth was tested using colorimetric and fluorescence microscopic assays. While solid biofilms were formed on glass surfaces, on the hydrophobic surface of gutta-percha points, no confluent but localised, spotty biofilms were observed. Furthermore, R. mucosa was able form biofilms on dentin. The data obtained indicate that R. mucosa can support establishment of endodontic biofilms and furthermore, infected root canals might serve as an entrance pathway for blood stream infections by this emerging pathogen.

  10. Ocular adnexal mucosa-associated lymphoid tissue lymphoma treated with radiotherapy

    International Nuclear Information System (INIS)

    Ejima, Yasuo; Sasaki, Ryohei; Okamoto, Yoshiaki; Maruta, Tsutomu; Azumi, Atsushi; Hayashi, Yoshitake; Demizu, Yusuke; Ota, Yosuke; Soejima, Toshinori; Sugimura, Kazuro

    2006-01-01

    Forty-two patients with stage IE ocular adnexal mucosa-associated lymphoid tissue (MALT) lymphoma were retrospectively analyzed. Five-year local control and progression-free survival rates were 100 and 77%, respectively. The most common relapsed site was the contralateral orbit. Thirty Gy of local irradiation seemed to be quite effective and safe

  11. Quantification of bacteria adherent to gastrointestinal mucosa by real-time PCR

    NARCIS (Netherlands)

    Huijsdens, Xander W.; Linskens, Ronald K.; Mak, Mariëtte; Meuwissen, Stephan G. M.; Vandenbroucke-Grauls, Christina M. J. E.; Savelkoul, Paul H. M.

    2002-01-01

    The use of real-time quantitative PCR (5' nuclease PCR assay) as a tool to study the gastrointestinal microflora that adheres to the colonic mucosa was evaluated. We developed primers and probes based on the 16S ribosomal DNA gene sequences for the detection of Escherichia coli and Bacteroides

  12. The Effect of Propolis in Healing Injured Nasal Mucosa: An Experimental Study

    Science.gov (United States)

    El-Anwar, Mohammad Waheed; Abdelmonem, Said; Abdelsameea, Ahmed A.; AlShawadfy, Mohamed; El-Kashishy, Kamal

    2016-01-01

    Introduction  Mechanical trauma to the nasal mucosa increases the risk of synechia formation, especially after chronic rhinosinusitis and nasal surgeries. Objective  This study was carried to assess the effect of propolis administration in healing injured nasal mucosa in rats. Methods  We randomly divided eighteen rats into three equal experimental groups: (1) non-treated group; (2) gum tragacanth (suspending agent for propolis) treated group; and (3) propolis treated group. The non-treated group received no treatment for 15 days. The second group received gum tragacanth administration (5 ml/kg, orally) once daily for 15 days. The third group received propolis suspension orally at a dose of 100 mg/kg once daily for 15 days. At the beginning of this study, we induced unilateral mechanical nasal trauma on the right nasal mucosa of all rats in the three groups using a brushing technique. A pathologist stained tissue samples using hematoxylin and examined eosin by using a light microscope. Results  The severity of inflammation was milder with the absence of ulcerations in the propolis treated group compared with the non-treated and gum tragacanth groups. Goblet cell and ciliated cell loss was substantially lower in patients treated with propolis compared with groups without treatment and those treated with gum tragacanth. Conclusion  Propolis decreased inflammation and enhanced healing of wounds of the nasal mucosa in rats. PMID:27413403

  13. The Effect of Propolis in Healing Injured Nasal Mucosa: An Experimental Study

    Directory of Open Access Journals (Sweden)

    El-Anwar, Mohammad Waheed

    2016-02-01

    Full Text Available Introduction Mechanical trauma to the nasal mucosa increases the risk of synechia formation, especially after chronic rhinosinusitis and nasal surgeries. Objective This study was carried to assess the effect of propolis administration in healing injured nasal mucosa in rats. Methods We randomly divided eighteen rats into three equal experimental groups: (1 non-treated group; (2 gum tragacanth (suspending agent for propolis treated group; and (3 propolis treated group. The non-treated group received no treatment for 15 days. The second group received gum tragacanth administration (5 ml/kg, orally once daily for 15 days. The third group received propolis suspension orally at a dose of 100 mg/kg once daily for 15 days. At the beginning of this study, we induced unilateral mechanical nasal trauma on the right nasal mucosa of all rats in the three groups using a brushing technique. A pathologist stained tissue samples using hematoxylin and examined eosin by using a light microscope. Results The severity of inflammation was milder with the absence of ulcerations in the propolis treated group compared with the non-treated and gum tragacanth groups. Goblet cell and ciliated cell loss was substantially lower in patients treated with propolis compared with groups without treatment and those treated with gum tragacanth. Conclusion Propolis decreased inflammation and enhanced healing of wounds of the nasal mucosa in rats.

  14. Heterotopic gastric mucosa associated with abdominal abscess formation, hypotension, and acute abdominal pain in a puppy.

    Science.gov (United States)

    Tobleman, Bridget N; Sinnott, Virginia B

    2014-01-01

    To describe the presence of heterotopic gastric mucosa forming an abscess associated with acute abdominal pain and shock in a puppy. A 7-month-old male intact Shih-Tzu was presented to the emergency service for evaluation of a 12-hour history of vomiting and lethargy progressing to weakness. On presentation, the puppy was obtunded and hypotensive. Radiographs revealed an ill-defined mid-ventral abdominal mass. Ultrasound revealed an echogenic, fluid-filled mass associated with the jejunum. The puppy had an exploratory celiotomy and a 2 × 4 cm oval fluid-filled soft tissue mass was found to be intimately associated, but not communicating with, a section of the mid-jejunum. The mass and associated jejunum were removed via enterectomy. Histopathology of the resected mass revealed heterotopic gastric mucosa; bacterial culture of the fluid contained in the mass revealed heavy growth of Escherichia coli. The puppy recovered from surgery, was discharged from the hospital, and has had no further complications from this episode. Heterotopic gastric mucosa is commonly found incidentally on necropsy. When it has been associated with acute gastrointestinal signs, mechanical intestinal obstruction with or without perforation was noted. To the authors' knowledge, this is the first reported case of heterotopic gastric mucosa leading to abscess formation and acute abdominal pain in the dog. © Veterinary Emergency and Critical Care Society 2014.

  15. NONINVASIVE NASAL MUCOSA EXPOSURE TO TREAT AND PREVENT RHINITIS AND RHINOSINUSITIS OF VARIOUS GENESIS

    Directory of Open Access Journals (Sweden)

    E. Yu. Radtsig

    2014-07-01

    Full Text Available The paper deals with the evaluation of the nasal mucosa in health and in different abnormalities. It describes the possibilities of using drugs of different groups to relieve symptoms in nasal mucosal and paranasal sinus abnormalities of different genesis, as well as the preventive effect of some of these drugs.

  16. Zonulin is not increased in the cardiac and esophageal mucosa of patients with gastroesophageal reflux disease.

    Science.gov (United States)

    Wex, Thomas; Mönkemüller, Klaus; Kuester, Doerthe; Fry, Lucia; Kandulski, Arne; Malfertheiner, Peter

    2009-06-01

    Human Zonulin, related to the Zonula occludens toxin of Vibrio cholerae, regulates intestinal permeability and is induced in inflammatory disorders of the lower GI tract. Gastroesophageal reflux disease (GERD) is associated with an impairment of epithelial barrier function. Here, we studied expression of zonulin in the gastroesophageal mucosa of 58 patients with typical reflux symptoms and 27 asymptomatic controls. During endoscopy, multiple biopsies from gastroesophageal mucosa were obtained for routine histopathology (Helicobacter pylori-status, inflammation) and gene expression analysis (immunohistochemistry, ELISA). Patients with GERD presented with typical histopathological alterations like elongation of papillae (P=0.015), basal cell hyperplasia (PZonulin was found to be expressed ubiquitously in gastroesophageal mucosa. Mucosal levels in controls ranged between 2.2 and 3.7 ng/microg total protein. Mean values were significantly higher in antrum (3.3+/-1.7 ng/microg) than cardia (2.7+/-1.2n g/microg) and esophagus (2.2+/-1.3 ng/microg) (Pzonulin expression in gastroesophageal mucosa. In conclusion, despite its established role for intestinal permeability, Zonulin seems not to be involved in the regulation of epithelial barrier function in relation to GERD.

  17. Perception of iron deficiency from oral mucosa alterations that show a high prevalence of Candida infection

    Directory of Open Access Journals (Sweden)

    Shin-Yu Lu

    2016-08-01

    Conclusion: The study demonstrates that oral mucosa alterations accompanying oral candidosis are a sensitive indicator of ID. All oral changes can be successfully ameliorated by iron therapy plus antifungals when candidosis exists. Investigating the origin of IDA is necessary, because it may be the first sign of a more serious disease, particularly malignancy.

  18. Insulin and thyroxine effect on /sup 32/P incorporation in the phospholipids of chicken intestinal mucosa

    Energy Technology Data Exchange (ETDEWEB)

    Aleksandrov, S; Lazarov, J [Akademiya na Selskostopanskite Nauki, Sofia-Kostinbrod (Bulgaria). Inst. po Zhivotnovydstvo

    1977-01-01

    Trials were conducted with 56-day-old broiler chickens. The effect was followed up of insulin and alloxan as well as of L-thyroxine and 6-methylthiouracil on /sup 32/P incorporation in phospholipids of the duodenal mucosa. A segment of the duodenum was isolated and Na/sub 2/H/sup 32/PO/sub 4/ was introduced therein. The lipids were extracted from duodenal mucosa and the individual phospholipids were separated by means of thin layer chromatography on sillica gel-G. Radioactivity was determined of individual phospholipid fractions. Blood glucose level was studied in insulin and alloxan-treated chickens. The inference was drawn that insulin significantly enhances /sup 32/P incorporation in the phospholipids in broiler chicken duodenal mucosa. The drop in blood glucose in insulin-treated chickens is inversely proportional to /sup 32/ P inclusion in individual phospholipids of duodenal mucosa. L-thyroxine exerts positive effect in chickens concerning /sup 32/P incorporation in lecithin and lysolecithin, this effect being negative with respect to sphingomyelin, cephalin and cardiolipin. Thyroid gland inhibition by 6-methylthiouracil induces negligible decline in /sup 32/P inclusion.

  19. Morphological study of the sensory innervation of the rat labial mucosa.

    Science.gov (United States)

    Yamamoto, T; Tazaki, M; Sakada, S

    1986-02-01

    The sensory innervation of the rat labial mucosa was investigated by means of methylene blue vital staining and osmic acid staining. Sensory receptors in this region were of three kinds (free nerve endings, encapsulated corpuscles and bush-like nerve endings) which constituted separate sensory units respectively. The encapsulated corpuscles were observed in the deep part of lamina propria, and distributed mainly in the margin of labial mucosa. Almost all (78.8%) of encapsulated corpuscles were of a simple type which had a non-branched axon terminal. No clew-like type corpuscles or glomerular-Meissner corpuscles were observed. The bush-like nerve endings were located in the lamina propria close to the epithelium, and localized in the central part of labial mucosa where the formation of papillae was remarkable. The density of the encapsulated corpuscles in the entire mucosa was 3.5-5.3/mm2, and that of the bush-like nerve endings in the densely distributed area was 38.9-60.6/mm2.

  20. Histopathological evaluation of urethroplasty with dorsal buccal mucosa: an experimental study in rabbits

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    Geovanne F. Souza

    2008-06-01

    Full Text Available PURPOSE: Buccal mucosa is a widely accepted tissue for urethroplasty. The exact healing and tissue integration process, mainly the histological characteristics of dorsal buccal mucosa graft urethroplasty when used dorsally to reconstruct the urethral plate has not previously been assessed, and thus we developed an experimental model to address this question. MATERIALS AND METHODS: In 12 New Zealand rabbits (weight 2.5 kg we surgically created a dorsal penile urethral defect. A buccal mucosa graft was sutured to the corpora and tunica albuginea, and the ventral urethra anastomosed to this new urethral plate. The animals were divided in three groups and sacrificed 1, 3 and 6 weeks after surgery (groups 1, 2 and 3. A retrograde urethrogram was obtained at autopsy in the last group and the penis analyzed histologically with hematoxylin-eosin and Masson's staining. RESULTS: The urethrograms showed no evidence of fistula or stricture. In group 1 the histopathological analysis showed submucosal lymph-mononuclear inflammatory edema, numerous eosinophils and squamous epithelium integrated into the adjacent urothelium. In group 2 there was no evidence of an inflammatory response but rather complete subepithelial hyaline healing, which was more marked in group 3. CONCLUSION: Healing of buccal mucosa grafts to reconstruct the urethral plate can be achieved by total integration of the squamous epithelium with the urothelium, maintaining the original histological properties of the graft with no fibrosis or retraction.

  1. [Comparative study of lymphoid follicles in mucosa of pharynx and mucosal associated lymphoid tissues in paranasal sinuses].

    Science.gov (United States)

    Zhai, Weigang; Yao, Min; Chen, Jue

    2013-08-01

    To study the relationship between the lymphoid follicles in mucous membrane of pharynx and mucosal associated lymphoid tissues (MALT). Ten folliculi obtained from 10 patients of follicular pharyngitis and mucosa taken form 10 patients of paranasal sinusitis were fixed in neutral formalin and embedded in paraffin. Sections were prepared, stained by H. E and by immunohistochemical method staining with S-100,and observe by light microscopy. We observed the morphology of lymphoid follicles in mucous membrane of pharynx with MALT in mucosa of paranasal sinusitis as the contrast. Lymphoid follicles in mucosa of pharynx compared with MALT in the mucosa of paranasal sinuses, there was no mantle zone, no typical germinal center and no mucosal epithelium, immunological staining with S-100 was week. The lymphoid follicles in mucosa of pharynx does not belong to the MALT.

  2. Dendritic cell-associated immune inflammation of cardiac mucosa: a possible factor in the formation of Barrett's esophagus.

    Science.gov (United States)

    Bobryshev, Yuri V; Tran, Dinh; Killingsworth, Murray C; Buckland, Michael; Lord, Reginald V N

    2009-03-01

    The development of Barrett's esophagus is poorly understood, but it has been suggested that cardiac mucosa is a precursor of intestinal type metaplasia and that inflammation of cardiac mucosa may play a role in the formation of Barrett's esophagus. The present study was undertaken to examine the presence and distribution of immune-inflammatory cells in cardiac mucosa, specifically focusing on dendritic cells because of their importance as regulators of immune reactions. Endoscopic biopsy specimens were obtained from 12 patients with cardiac mucosa without Barrett's esophagus or adenocarcinoma and from 21 patients with Barrett's esophagus without dysplasia (intestinal metaplasia). According to histology, in nine of the 21 specimens with Barrett's esophagus, areas of mucosa composed of cardiac type epithelium-lined glands were present as well. Immunohistochemical staining and electron microscopy were used to examine immune-inflammatory cells in paraffin-embedded sections. Immune-inflammatory cells, including T cells, B cells, dendritic cells, macrophages, and mast cells, were present in the connective tissue matrix that surrounded cardiac type epithelium-lined glands in all patients with cardiac mucosa. Clustering of dendritic cells with each other and with lymphocytes and the intrusion of dendritic cells between glandular mucus cells were observed. In the Barrett's esophagus specimens that contained cardiac type glands, computerized CD83 expression quantitation revealed that there were more dendritic cells in cardiac mucosa than in intestinal metaplasia. Immune-inflammatory infiltrates containing dendritic cells are consistently present in cardiac mucosa. The finding of a larger number of dendritic cells in areas of cardiac mucosa in Barrett's esophagus biopsies suggests that the immune inflammation of cardiac mucosa might play a role in modifying the local tissue environment to promote the development of specialized intestinal type metaplasia.

  3. Culturas del Mundo

    DEFF Research Database (Denmark)

    Benwell, Ann Fenger; Costa, Alberto; Waehle, Espen

    2006-01-01

    ’Culturas del mundo. Colecciones del Museo Nacional de Dinamarca’ with Ann Fenger Benwell in Culturas del Mundo. Colecciones del Museo Nacional de Dinamarca, ed. Silvia Sauquet, Fundación "la Caixa", Barcelona 2006, pp. 31-39......’Culturas del mundo. Colecciones del Museo Nacional de Dinamarca’ with Ann Fenger Benwell in Culturas del Mundo. Colecciones del Museo Nacional de Dinamarca, ed. Silvia Sauquet, Fundación "la Caixa", Barcelona 2006, pp. 31-39...

  4. Symptoms and signs in individuals with serology positive for celiac disease but normal mucosa

    Directory of Open Access Journals (Sweden)

    Brandt Lena

    2009-07-01

    Full Text Available Abstract Background Antibody serology is an important tool in the investigation of celiac disease (CD, but does not always correlate with mucosal appearance in the small intestine. Patients with positive CD serology but normal mucosa (Marsh 0 are at increased risk of future CD. In this study we describe a model for identifying and characterizing individuals with normal mucosa but positive CD serology. Such individuals are sometimes referred to as having latent CD. Methods The records of ten Swedish pathology departments were used to identify individuals with biopsies indicating normal duodenal/jejunal mucosa. Using the national personal identification number, these data were linked with CD serology data (antigliadin, antiendomysial and tissue transglutaminase antibodies; and we thereby identified 3,736 individuals with normal mucosa but positive CD serology. Two independent reviewers then manually reviewed their biopsy reports to estimate comorbidity. We also randomly selected 112 individuals for validation through patient chart review. Results The majority of the 3,736 individuals were females (62%. Children (0–15 years made up 21.4%. The median number of biopsy specimen was 3. Our review of biopsy reports found that other gastrointestinal comorbidity was rare (inflammatory bowel disease: 0.4%; helicobacter pylori infection: 0.2%. Some 22% individuals selected for patient chart review had a relative with CD. The most common symptoms among these individuals were diarrhea (46% and abdominal pain (45%, while 26% had anemia. Although 27% of the individuals selected for validation had been informed about gluten-free diet, only 13% were adhering to a gluten-free diet at the end of follow-up. Conclusion Individuals with positive CD serology but normal mucosa often have CD-like symptoms and a family history of CD.

  5. Carprofen-induced oxidative stress in mitochondria of the colonic mucosa of the dog.

    Science.gov (United States)

    Snow, Lynne A; McConnico, Rebecca S; Morgan, Timothy W; Hartmann, Erica; Davidson, Jacqueline R; Hosgood, Giselle

    2014-07-01

    The purpose of the study was to compare the conductance and mannitol permeability of canine colonic mucosa in response to carprofen or 2,4-dinitrophenol (DNP) with or without tempol pretreatment. Ten colonic mucosa sections per dog were mounted in Ussing chambers. Treatments were done in duplicate. Mucosa was exposed to carprofen (200 μg/mL) or DNP (0.25 mM), both with and without tempol (1 mM) pretreatment. Conductance was calculated every 15 min for 240 min. Mannitol flux was calculated over 3 consecutive 60-minute periods. Histology or electron microscopy was done after exposure. Conductance over time, mannitol flux, frequency of histologic categories, and electron microscopic changes were analyzed for treatment effects. The mean ± standard deviation (SD) conductance over time for carprofen or DNP-treated colons was not significantly different from control regardless of tempol pretreatment. Period 3 mannitol fluxes for carprofen and DNP-treated colon were not significantly different, but were greater than control. Period 3 mannitol flux for tempol + carprofen was significantly less than tempol + DNP-treated colon. Sloughing of cells and erosions were seen in the mucosa of carprofen-treated colon. Mitochondrial damage was seen more often in carprofen-treated than DNP-treated or control colon. Tempol pretreatment resulted in more ruptured mitochondria in the carprofen-treated colon; however, other mitochondrial changes were not significantly affected by tempol pretreatment in either carprofen or DNP treated colon. Treatment with carprofen or DNP increased the mannitol flux, but pretreatment with tempol mitigated the carprofen effect. It is apparent that structural mitochondrial damage occurs in the canine colonic mucosa after carprofen and DNP exposure.

  6. Substitution urethroplasty using oral mucosa graft for male anterior urethral stricture disease: Current topics and reviews.

    Science.gov (United States)

    Horiguchi, Akio

    2017-07-01

    Male anterior urethral stricture is scarring of the subepithelial tissue of the corpus spongiosum that constricts the urethral lumen, decreasing the urinary stream. Its surgical management is a challenging problem, and has changed dramatically in the past several decades. Open surgical repair using grafts or flaps, called substitution urethroplasty, has become the gold standard procedure for anterior urethral strictures that are not amenable to excision and primary anastomosis. Oral mucosa harvested from the inner cheek (buccal mucosa) is an ideal material, and is most commonly used for substitution urethroplasty, and lingual mucosa harvested from the underside of the tongue has recently emerged as an alternative material with equivalent outcome. Onlay augmentation of oral mucosa graft on the ventral side (ventral onlay) or dorsal side (dorsal onlay, Barbagli procedure) has been widely used for bulbar urethral stricture with comparable success rates. In bulbar urethral strictures containing obliterative or nearly obliterative segments, either a two-sided dorsal plus ventral onlay (Palminteri technique) or a combination of excision and primary anastomosis and onlay augmentation (augmented anastomotic urethroplasty) are the procedures of choice. Most penile urethral strictures can be repaired in a one-stage procedure either by dorsal inlay with ventral sagittal urethrotomy (Asopa technique) or dorsolateral onlay with one-sided urethral dissection (Kulkarni technique); however, staged urethroplasty remains the procedure of choice for complex strictures, including strictures associated with genital lichen sclerosus or failed hypospadias. This article presents an overview of substitution urethroplasty using oral mucosa graft, and reviews current topics. © 2017 The Japanese Urological Association.

  7. Inducing effect of clofibric acid on stearoyl-CoA desaturase in intestinal mucosa of rats.

    Science.gov (United States)

    Yamazaki, Tohru; Kadokura, Makiko; Mutoh, Yuki; Sakamoto, Takeshi; Okazaki, Mari; Mitsumoto, Atsushi; Kawashima, Yoichi; Kudo, Naomi

    2014-12-01

    Fibrates have been reported to elevate the hepatic proportion of oleic acid (18:1n-9) through inducing stearoyl-CoA desaturase (SCD). Despite abundant studies on the regulation of SCD in the liver, little is known about this issue in the small intestine. The present study aimed to investigate the effect of clofibric acid on the fatty acid profile, particularly monounsaturated fatty acids (MUFA), and the SCD expression in intestinal mucosa. Treatment of rats with a diet containing 0.5% (w/w) clofibric acid for 7 days changed the MUFA profile of total lipids in intestinal mucosa; the proportion of 18:1n-9 was significantly increased, whereas those of palmitoleic (16:1n-7) and cis-vaccenic (18:1n-7) acids were not changed. Upon the treatment with clofibric acid, SCD was induced and the gene expression of SCD1, SCD2, and fatty acid elongase (Elovl) 6 was up-regulated, but that of Elovl5 was unaffected. Fat-free diet feeding for 28 days increased the proportions of 16:1n-7 and 18:1n-7, but did not effectively change that of 18:1n-9, in intestinal mucosa. Fat-free diet feeding up-regulated the gene expression of SCD1, but not that of SCD2, Elovl6, or Elovl5. These results indicate that intestinal mucosa significantly changes its MUFA profile in response to challenges by clofibric acid and a fat-free diet and suggest that up-regulation of the gene expression of SCD along with Elovl6 is indispensable to elevate the proportion of 18:1n-9 in intestinal mucosa.

  8. Transcription factors GATA-4 and GATA-6 in normal and neoplastic human gastrointestinal mucosa

    Directory of Open Access Journals (Sweden)

    Mäki Markku

    2008-04-01

    Full Text Available Abstract Background Human gastrointestinal mucosa regenerates vigorously throughout life, but the factors controlling cell fate in mature mucosa are poorly understood. GATA transcription factors direct cell proliferation and differentiation in many organs, and are implicated in tumorigenesis. GATA-4 and GATA-6 are considered crucial for the formation of murine gastrointestinal mucosa, but their role in human gastrointestinal tract remains unexplored. We studied in detail the expression patterns of these two GATA factors and a GATA-6 down-stream target, Indian hedgehog (Ihh, in normal human gastrointestinal mucosa. Since these factors are considered important for proliferation and differentiation, we also explored the possible alterations in their expression in gastrointestinal neoplasias. The expression of the carcinogenesis-related protein Indian hedgehog was also investigated in comparison to GATA factors. Methods Samples of normal and neoplastic gastrointestinal tract from children and adults were subjected to RNA in situ hybridization with 33P labelled probes and immunohistochemistry, using an avidin-biotin immunoperoxidase system. The pathological tissues examined included samples of chronic and atrophic gastritis as well as adenomas and adenocarcinomas of the colon and rectum. Results GATA-4 was abundant in the differentiated epithelial cells of the proximal parts of the gastrointestinal tract but was absent from the distal parts. In contrast, GATA-6 was expressed throughout the gastrointestinal epithelium, and in the distal gut its expression was most intense at the bottom of the crypts, i.e. cells with proliferative capacity. Both factors were also present in Barrett's esophagus and metaplasia of the stomach. GATA-6 expression was reduced in colon carcinoma. Ihh expression overlapped with that of GATA-6 especially in benign gastrointestinal neoplasias. Conclusion The results suggest differential but overlapping functions for GATA-4 and

  9. Early remodeling of nasal mucosa in rat model after radiation injury

    International Nuclear Information System (INIS)

    Xiao Mang; Tang Jianguo; Luo Baozhen; Zhao Li'na; Shi Guozhi

    2008-01-01

    Objective: To explore the feature of nasal mucosa remodeling in experimental radiation injury. Methods: Fourty male rats were randomly divided into five groups, as control group and radiation injury groups (radiation dose were 20 Gy, 30 Gy, 40 Gy and 50 Gy). Each group had 8 rats. Two weeks after the last irradiation, the rats were killed and the nasal middle turbinates of the animals were removed. The tissue blocks were embedded in paraffin. The paraffin sections were stained with hematoxylin and eosin (HE), alcian blue- periodic acid-Schif (AB-PAS), and Masson Trichrome (MT). The infiltrating eosinophils in nasal mucosa were examined. AB-PAS positive cells in the surface epithelium in nasal mucosa were counted. The percentage of area in MT stained extracellular matrix in nasal mucosa and damage of epithelium were determined by an image analyzer. Results: The control group only presented a few eosinophils. Significant eosinophil infiltration was observed in the radiation injury groups, especially for the 30 Gy radiation injury group. Compared with the control group, there was no significant epithelial damage in 20 Gy radiation injury group. Significant epithelial damage were observed in the rest of radiation injury groups. The epithelial damage became more severe as the radiation dose increasing. A little but not significant increase in AB-PAS positive cells was observed in the mucos of the 20 Gy radiation injury group and significant increase in the 30 and 40 Gy groups. But in the 50 Gy radiation injury group, the AB-PAS positive cells were decreased compared with control group. The collagen fibrils in the mucosa of nasal middle turbinate in 20 Gy radiation injury group did not significantly increase.. But in the other groups, the increase was significant compared with that of control group. Furthermore, collagen fibrils increased as the radiation dose increased. Conclusions: Epithelial damage, goblet cells hyperplasia and extracellular matrix deposition are the

  10. Effects of alanyl-glutamine supplementation on the small intestinal mucosa barrier in weaned piglets

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    Shen Xing

    2017-02-01

    Full Text Available Objective The study was to investigate the effects of alanyl-glutamine (Ala-Gln and glutamine (Gln supplementation on the intestinal mucosa barrier in piglets. Methods A total of 180 barrows with initial weight 10.01±0.03 kg were randomly allocated to three treatments, and each treatment consisted of three pens and twenty pigs per pen. The piglets of three groups were fed with control diet [0.62% alanine (Ala], Ala-Gln diet (0.5% Ala-Gln, Gln diet (0.34% Gln and 0.21% Ala, respectively. Results The results showed that in comparison with control diet, dietary Ala-Gln supplementation increased the height of villi in duodenum and jejunum (p<0.05, Gln supplementation increased the villi height of jejunum (p<0.05, Ala-Gln supplementation up-regulated the mRNA expressions of epidermal growth factor receptor and insulin-like growth factor 1 receptor in jejunal mucosa (p<0.05, raised the mRNA expressions of Claudin-1, Occludin, zonula occludens protein-1 (ZO-1 and the protein levels of Occludin, ZO-1 in jejunal mucosa (p<0.05, Ala-Gln supplementation enlarged the number of goblet cells in duodenal and ileal epithelium (p<0.05, Gln increased the number of goblet cells in duodenal epithelium (p<0.05 and Ala-Gln supplementation improved the concentrations of secretory immunoglobulin A and immunoglobulin G in the jejunal mucosa (p<0.05. Conclusion These results demonstrated that dietary Ala-Gln supplementation could maintain the integrity of small intestine and promote the functions of intestinal mucosa barriers in piglets.

  11. A preliminary report on the effects of paclitaxel-impregnated stents on sheep nasal mucosa.

    Science.gov (United States)

    Herrmann, Brian W; Citardi, Martin J; Vogler, George; Gardner, Laura; Smith, Greg; Javer, Amin R; Burt, Helen M; Jackson, John; Kuhn, Frederick A

    2004-01-01

    Traditional frontal sinus stents serve only as mechanical devices. It has been proposed that stents also may serve as drug-delivery systems for the topical application of drugs that minimize postoperative scarring. Paclitaxel (Taxol), which has recognized antiscarring effects, may be incorporated via a polymeric formulation into standard rubber stents. The impact of topically applied paclitaxel on the morphology of the nasal mucosa is unknown. An adult sheep model was used for this study. A modified rubber T-tube stent (incorporating paclitaxel at varying dosages) was secured to each side of the septum in four animals (eight sides). An unmodified T-tube was placed on each side of one animal, a T-tube with the drug carrier (but no paclitaxel) was placed on each side of the second animal, and T-tubes with varying paclitaxel were placed on each side of the final two animals. After 4 weeks, animals were killed and the nasal mucosa was harvested. The nasal mucosa was sectioned and stained with hematoxylin and eosin. A pathologist then assessed the nasal mucosa for vascular congestion, glandular atrophy, chronic inflammation, mucosal metaplasia, and mucosal ulceration. No consistent histopathological differences were noted in the specimens. All specimens showed varying degrees of vascular congestion, glandular atrophy, chronic inflammation, and mucosal metaplasia; the paclitaxel-impregnated stents were not consistently associated with more severe mucosal injury. Finally, mucosal ulceration was noted to be very rare in all specimens. This preliminary report describes the impact of paclitaxel-impregnated stents on sheep nasal mucosa, which tolerated these stents very well. Because paclitaxel minimizes scarring reactions at very low concentrations, paclitaxel-impregnated stents may prove useful in clinical situations in which frontal sinus stenting is deemed necessary. Additional investigations with animal models, as well as clinical trials, may be warranted.

  12. Microbiota, infecciones gastrointestinales, inflamación de bajo grado y antibioticoterapia en el síndrome de intestino irritable. Una revisión basada en evidencias

    Directory of Open Access Journals (Sweden)

    M. Schmulson

    2014-04-01

    Conclusiones: Se requieren más estudios para determinar la microbiota intestinal propia del SII y las diferencias en inflamación de bajo grado entre SII-PI y SII-No PI. La rifaximina ha demostrado efectividad en el tratamiento del SII independientemente de los factores anteriores.

  13. Tumor estromal gastrointestinal de intestino delgado

    Directory of Open Access Journals (Sweden)

    Francisco Marivaldo Benício da Silva

    Full Text Available Stromal tumors of the gastrointestinal tract (gists represent relatively rare lesions that arise from connective tissue elements located along the entire length of the gut. They were initially identified by immunohistochemical investigation, proving their origin from nondifferentiated mesenchymal cells. Only a minority of this lesions, mainly those confined to the esophagus and rectum, have been shown to correspond to mature, well-differentiated types of neoplasms such as leiomyoma or leiomyosarcoma. The majority of gists corresponds to a heterogeneous group of lesions that have as their common denominator an immature proliferation of epithelioid or spindle cells arising from its muscle layers, or between them, showing partial or incomplete myoide, neural, ganglionic, or mixed features of differentiation. This case report intends to show a gist of small bowel in a male, 46 years old, with a two-year of evolution.

  14. The role of gut-liver axis in the restriction of intrauterine growth in a model of experimental gastroschisis O papel do eixo intestino-fígado na restrição de crescimento intra-uterino em um modelo de gastrosquise experimental

    Directory of Open Access Journals (Sweden)

    Márcia Pereira Bueno

    2013-01-01

    Full Text Available PURPOSE: To evaluate the intrauterine growth restriction (IUGR by the expression of IR-β, IRS-1, IRS-2, IGF-IRβ and Ikappaβ in experimental model of gastroschisis. METHODS: Pregnant rats at 18.5 days of gestation were submitted to surgery to create experimental fetal gastroschisis (term = 22 days were divided in three groups: gastroschisis (G, control (C and sham (S. Fetuses were evaluated for body weight (BW, intestinal (IW, liver (LW and their relations IW/BW and LW/BW. IR-β and IGF-IRβ receptors, IRS-1 and IRS-2 substrates and Ikappaβ protein were analyzed by western blotting. RESULTS: BW was lower in G, the IW and IW / BW were greater than C and S (pOBJETIVO: Avaliar a restrição de crescimento intra-uterino (RCIU pela expressão de IR-β, IRS-1, IRS-2, IGF-IRβ e a via inflamatória do Ikappaβ no modelo de gastrosquise experimental. MÉTODOS: Ratas grávidas com 18,5 dias de gestação foram submetidas a cirurgia experimental para criar gastrosquise fetal (termo = 22 dias e os fetos foram divididos em três grupos: gastrosquise (G, controle (C e sham (S. Os fetos foram avaliados quanto ao peso corporal (BW, intestinal (IW, fígado (LW e suas relações IW/BW e LW/BW. Os receptores IR-β e IGF-IRβ, os substratos IRS-1 e IRS-2 e a proteína Ikappaβ foram analisados por western blotting. RESULTADOS: O BW de G foi menor, o IW e IW/BW foram superiores a C e S (p < 0.05. O fígado não apresentou diferenças entre os grupos. Nos fetos com gastrosquise, quando comparados com fetos controles, a expressão de IGF-IRβ (p<0.001 e Ikappaβ (p<0.001 aumentou no fígado e intestino, assim como IR-β (p<0.001 que diminuiu em ambos. Inversamente ao intestino, IRS-1 (p<0.001 aumentou no fígado e IRS-2 diminuiu (p<0.01. CONCLUSÃO: O eixo do intestino fígado tem um papel importante na inflamação, com consequentes alterações na via metabólica de glicose que pode contribuir para a RCIU em fetos com gastrosquise.

  15. Tricholeiperia peruensis n. sp. (Nematoda, Molineidae del quiróptero Lophostoma silvicolum occidentalis (Phyllostomidae en Tumbes, Perú

    Directory of Open Access Journals (Sweden)

    Marina Vargas

    2008-12-01

    Full Text Available En el presente trabajo, se describe a Tricholeiperia peruensis n. sp. (Nematoda, Molineidae en base a 25 especímenes colectados del intestino de 5 murciélagos de la especie Lophostoma silvicolum occidentalis de la localidad de Angostura, distrito Pampas de Hospital, departamento de Tumbes, Perú. La nueva especie se caracteriza por la morfología de la vesícula cefálica en forma de capuchón con escotaduras, por el tamaño y forma de las espículas cuyo extremo distal de la rama externa se divide en dos, de las cuales la rama interna sufre una nueva división; por la disposición de las costillas de la bolsa copulatríz donde, entre otros detalles, las antero y postero-ventrales están separadas y las externo-dorsales nacen cerca de la parte media del tronco dorsal y son gruesas.

  16. The relationship of Candida colonization of the oral and vaginal mucosae of mothers and oral mucosae of their newborns at birth.

    Science.gov (United States)

    Al-Rusan, Rund M; Darwazeh, Azmi M G; Lataifeh, Isam M

    2017-04-01

    Vaginal Candida colonization is common during pregnancy. Vaginal Candida may transmit vertically to the mouth of newborns during labor. The aim of this study was to assess and compare oral Candida colonization between vaginally born newborns and cesarean-born newborns and to investigate the association of the mother's vaginal and oral Candida colonization and the newborn's oral colonization at the time of delivery. Culture swabs were collected from the oral and vaginal mucosae of 100 pregnant women and from the oral mucosa of their 100 full-term newborns. Fifty (50%) of the mothers gave birth vaginally and the other 50 (50%) by cesarean section. The prevalence of oral and vaginal Candida in pregnant mothers was 49% and 40%, respectively. Oral Candida colonization in newborns was 7%. Oral Candida was isolated from 5 of 50 (10%) in the vaginally born group and from 2 of 50 (4%) in the cesarean-born group (P = .44). In vaginally born group, oral Candida was isolated from 5 of 20 (25%) in those born to mothers with vaginal colonization of Candida, and 0 of 30 (0.0%) in mothers without vaginal colonization of Candida (P = .007). The mother's vaginal Candida may constitute an important source of oral Candida in the newborns, particularly in those delivered vaginally. Copyright © 2017 Elsevier Inc. All rights reserved.

  17. Estudio de las alteraciones del esmalte en la enfermedad celiaca

    OpenAIRE

    Bonet Coloma, Cristina

    2013-01-01

    La enfermedad celiaca (EC) es una enteropatía caracterizada por una intolerancia permanente al gluten, proteína existente en el trigo, centeno, cebada y avena que provoca una lesión severa de las vellosidades intestinales, y se normaliza tras su retirada de la dieta. La sintomatología varía de un paciente a otro dependiendo del daño de la mucosa intestinal. La presentación típica incluye diarrea, pérdida de peso, fatiga, distensión abdominal, vómitos, deficiencias nutricionales y anemia p...

  18. Methadone as an inducer of apoptotic process in cheek mucosae cells in rats

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    Małgorzata Stępień

    2017-11-01

    Full Text Available Methadone is an opioid medication which can reduce withdrawal symptoms in people addicted to heroin and other drugs. Methadone is used also as a pain reliever and as part of drug addiction detoxification program. Apoptosis is the physiological process that plays a critical role in development and tissue homeostasis. The progress of apoptosis is regulated by signal cascades. The aim of this study was to asses how methadone induces apoptotic process in cheek mucosae cells in rats. Forty albino rats wares divided into two parts and five subgroups each. The biggest histological changes of cheek mucosae was observed in the groups with methadone. There is no indication of ability to regeneration in short time after treatment.

  19. DETECTION OF Helicobacter pylori IN GASTRIC MUCOSA OF SHEEP: PRELIMINARY RESULTS

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    A. Rella

    2013-02-01

    Full Text Available Helicobacter pylori is an organism widespread in humans and sometimes responsible for serious illnesses. It has been hypothesized the existence of animal reservoirs, and that the infection route by H. pylori involves multiple pathways including food-borne transmission as the microorganism has been detected from sheep, goat and cow milk. This work reports the preliminary results of a survey conducted in order to investigate the presence of H. pylori in gastric mucosa of sheep slaughtered in Apulia region (Italy employing a Nested Polymerase Chain Reaction (Nested-PCR assay for the detection of the phosphoglucosamine mutase gene (glmM, as screening method followed by conventional bacteriological isolation. Out of the 50 gastric mucosa samples examined, 3 (6% resulted positive for the presence of glmMgene, but at this time no strains were isolated. The results deserve further investigations to asses the role of ruminants as possible reservoirs of H. pylori.

  20. The effect of radon 222 on the oral mucosa of rabbits

    International Nuclear Information System (INIS)

    Minta, A.; Minta, P.; Kochanski, W.

    1975-01-01

    In experiments on 52 rabbits the authors investigated the effects of radon 222 administered by inhalation on the oral mucosa. The experimental animals were divided into 3 groups: 1 - controls, 2 - receiving radon inhalations in concentration 1 nCi/1, 3- receiving similar inhalations in a concentration of 5 nCi/l of air. Sections involving the lower lip with the mucosa were obtained for investigations after 10, 20, 30 and 60 days of inhalation. In the group of animals receiving radon in lower concentration its stimulating effect manifested itself as stimulation of mesenchymal cells and vascular endothelium. In group 3 sebaceous glands, atrophy and excessive keratinization of epidermis. Taking into account the analogy of these processes the authors concluded that in balneotherapy of oral diseases radon water application with low content of radon 222 may be satisfactory and safe. (author)

  1. Phantom gastric mucosa for evaluating computed radiography in double-contrast upper gastrointestinal examinations

    International Nuclear Information System (INIS)

    Murakami, M.; Watanabe, H.; Nakata, H.

    1996-01-01

    To test the clinical usefulness of computed radiography (CR) with a storage phosphor plate in upper gastrointestinal radiographic examinations, a newly devised phantom gastric mucosa was used. Simulated small elevated and depressed lesions were created on a phantom gastric mucosa made from a styrofoam 'plate'. Twenty-four sets of each CR and screen-film radiographs (SR) were obtained using phototimed exposures. Receiver operating characteristic (ROC) study and visual ranking using these images were performed. There was no significant difference between the ROC curves of CR and SR. By visual ranking, CR was equal to or better than SR in most cases. In no case was SR definitely superior to CR. CR can be safely applied in upper gastrointestinal roentgenologic examinations. (orig.)

  2. Confocal Laser Endomicroscopy in the Study of Colonic Mucosa in IBD Patients: A Review

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    Francesca Salvatori

    2012-01-01

    Full Text Available Confocal laser endomicroscopy (CLE is one of several novel methods that provide real-time, high-resolution imaging at a micronscale via endoscopes. CLE and related technologies are often termed “virtual biopsy” as they simulate the images seen in traditional histology. Recently, the use of CLE was reported in the study of colonic mucosa in patients with inflammatory bowel diseases and in particular in patients affected by ulcerative colitis. CLE has the potential to have an important role in management of IBD patients as it can be used to assess the grading of colitis and in detection of microscopic colitis in endoscopically silent segments. Moreover, CLE can be used in surveillance programs especially in high-risk patients. This report aims to evaluate the current data on the application of confocal endomicroscopy in clinical gastroenterology and particularly in the study of colonic mucosa in UC patients.

  3. The cytological status of the nasal mucosa and the buccal epithelium in coal miners

    Energy Technology Data Exchange (ETDEWEB)

    L.T. Bazeliuk; B.M. Salimbaeva

    2006-11-15

    Sixty-four persons were examined. The examination was undertaken to study the cytomophological parameters of the cells of the nasal mucosa and the buccal epithelium in coal miners. Group 1 consisted of 18 donors without contact with industrial dust; Group 2 comprised 24 apparently healthy miners; Group 3 included 22 workers (drift miners) with Stage 1 anthracosilicosis, grade 1 respiratory failure. The patients with Stage 1 anthracosilicosis had noticeably worse morphofunctional characteristics of the epithelium that displayed extensive fields with pronounced structural changes, such as destruction and desquamation of the integumentary epithelium of the nasal mucosa. Nasal mucosal atrophic changes were observed in 50% of the examined miners. Examination of the buccal epithelium in apparently healthy miners (code 0) and in workers with Stage I anthracosilicosis revealed the increased proportion of microfloral (Streptococcus) contamination by 79% and 3.7 times, respectively.

  4. Gene expression profiling of gastric mucosa in mice lacking CCK and gastrin receptors

    DEFF Research Database (Denmark)

    Zhao, Chun-Mei; Kodama, Yosuke; Flatberg, Arnar

    2014-01-01

    normalized, which was associated with an up-regulated pituitary adenylate cyclase-activating polypeptide (PACAP) type 1 receptor (PAC1). The basal part of the gastric mucosa expressed parathyroid hormone-like hormone (PTHLH) in a subpopulation of likely ECL cells (and possibly other cells) and vitamin D3 1α...... suggest a possible link between gastric PTHLH and vitamin D and bone metabolism.......The stomach produces acid, which may play an important role in the regulation of bone homeostasis. The aim of this study was to reveal signaling pathways in the gastric mucosa that involve the acid secretion and possibly the bone metabolism in CCK1 and/or CCK2 receptor knockout (KO) mice. Gastric...

  5. Isolation and identification of Helicobacter spp, from canine and feline gastric mucosa

    DEFF Research Database (Denmark)

    Jalava, K.; On, Stephen L.W.; VanDamme, P.A.R.

    1998-01-01

    It is known that virtually all healthy adult dogs and cats harbor spiral helicobacters in their gastric mucosa, Three species, Helicobacter felis, Helicobacter bizzozeronii, and Helicobacter salomonis have been isolated in vitro from the gastric mucosa of these animals. The aims of this study were...... conventional phenotypic tests, whole-cell protein profiling, and ultrastructural analysis in identifying the different species isolated from canine and feline gastric mucose. We cultured 95 and 22 gastric mucosal biopsies from dogs and cats, respectively. Twenty-one H. bizzozeronii strains, 8 H. felis strains......, 8 H. salomonis strains, 3 mixed cultures, 2 "Flexispira rappini"-like organisms, and 3 as get uncharacterized strains were isolated from the dogs, and 3 H. felis strains were isolated from the cats. The methods used here yielded Helicobacter isolation rates of 51% from dogs and 13.6% from cats...

  6. Ethanol as an inducer of apoptotic process in cheek mucosae in rats

    Directory of Open Access Journals (Sweden)

    Katarzyna Borowska

    2017-11-01

    Full Text Available Apoptosis is the process that plays a important role in development and tissue homeostasis. This physiological process is regulated by caspases. The caspases are specific cysteine proteases. The aim of this study was to prove how ethanol induces apoptotic process in cheek mucosae cells in rats. Fifteen male Wistar rats were used in the research. They were divided into two treated groups (group A and group Abis and control group. The biggest histological changes of cheek mucosae was observed in group with ethanol four weeks after last consumption. There is no indication of ability to regeneration in short time after treatment. The most marked was expression of caspase 8 in group A bis. In caspase 9 expression group A was more visible.

  7. Endoscopic OCT for in-vivo imaging of precancer and cancer states of human mucosa

    Science.gov (United States)

    Sergeev, Alexander M.; Gelikonov, Valentin M.; Gelikonov, Grigory V.; Feldchtein, Felix I.; Kuranov, Roman V.; Gladkova, Natalia D.; Shakhova, Natalia M.; Kuznetzova, Irina N.; Snopova, Ludmila; Denisenko, Arkady; Almasov, Valentin

    1998-01-01

    First results of endoscopic applications of optical coherence tomography for in vivo studies of human mucosa in gastrointestinal and genital tracts are presented. A novel endoscopic OCT system has ben created that is based on the integration of a sampling arm of an all-optical-fiber interferometer into standard endoscopic devices using their biopsy channel to transmit low-coherence radiation to investigated tissue. We have studied mucous membranes of esophagus, stomach and uterine cervix as typical localization for carcinomatous processes. Images of tumor tissues versus healthy tissues have been recorded and analyzed. Violations of well-defined stratified healthy mucosa structure in cancerous tissue is distinctly seen by EOCT, thus making this technique promising for early diagnosis of tumors and precise guiding of excisional biopsy.

  8. In vivo endoscopic OCT imaging of precancer and cancer states of human mucosa

    Science.gov (United States)

    Sergeev, Alexander M.; Gelikonov, V. M.; Gelikonov, G. V.; Feldchtein, Felix I.; Kuranov, R. V.; Gladkova, N. D.; Shakhova, N. M.; Snopova, L. B.; Shakhov, A. V.; Kuznetzova, I. A.; Denisenko, A. N.; Pochinko, V. V.; Chumakov, Yu P.; Streltzova, O. S.

    1997-12-01

    First results of endoscopic applications of optical coherence tomography for in vivo studies of human mucosa in respiratory, gastrointestinal, urinary and genital tracts are presented. A novel endoscopic OCT (EOCT) system has been created that is based on the integration of a sampling arm of an all-optical-fiber interferometer into standard endoscopic devices using their biopsy channel to transmit low-coherence radiation to investigated tissue. We have studied mucous membranes of esophagus, larynx, stomach, urinary bladder, uterine cervix and body as typical localization for carcinomatous processes. Images of tumor tissues versus healthy tissues have been recorded and analyzed. Violations of well-defined stratified healthy mucosa structure in cancered tissue are distinctly seen by EOCT, thus making this technique promising for early diagnosis of tumors and precise guiding of excisional biopsy.

  9. [Oral medicine 9. Lichen planus and lichenoid lesions of the oral mucosa].

    Science.gov (United States)

    van der Meij, E H; Schepman, K P; de Visscher, J G A M

    2013-09-01

    The general dentist is sometimes confronted with white lesions of the oral mucosa. Oral lichen planus is the most common oral white lesion. The diagnosis can usually be made on the basis of the clinical aspect, but is sometimes made more difficult by certain abnormalities in the oral mucosa which clinically resemble oral lichen planus or by abnormalities which cannot be distinguished from oral lichen planus but have a different origin. Those lesions are classified as oral lichenoid lesions. Malignant deterioration has been described in allforms of oral lichen planus lesions and oral lichenoid lesions. There is no known method to predict or prevent malignant transformation. Nor are there any studies examining the efficacy of frequent follow-up visits. It seems sensible, in keeping with the tendency in recent literature, to schedule annual check-ups for patients to be on the safe side. These follow-up visits may reasonably be performed in a general dental practice.

  10. Arrowroot as a treatment for diarrhoea in irritable bowel syndrome patients: a pilot study Araruta como um tratamento para a diarréia em pacientes com síndrome do intestino irritável: um estudo piloto

    Directory of Open Access Journals (Sweden)

    Caroline COOKE

    2000-01-01

    Full Text Available Objectives - Arrowroot is an old-fashioned remedy for diarrhoea, but no clinical studies have been done to evaluate its effectiveness. The aim of this pilot study was to assess its efficacy as a treatment for diarrhoea in 11 patients, all of whom had irritable bowel syndrome with diarrhoea as a feature. Methods - The patients were interviewed and a questionnaire completed on entry into the trial. They then took 10 mL arrowroot powder three times a day for one month and discontinued the treatment for the subsequent month. Questionnaires were completed after one month on treatment and at the end of the trial after one month off treatment. Results - Arrowroot reduced diarrhoea and had a long-term effect on constipation. It also eased abdominal pain. Conclusion - Arrowroot is an effective treatment for diarrhoea. Its action could be explained by several theories which relate to an increase in faecal bulk and thus a more efficient bowel action. The number of patients was small, and further studies are needed to substantiate preliminary results.Objetivos - Araruta é um antigo (absoleto remédio usado para a diarréia, porém estudos clínicos não têm sido realizados para avaliar sua efetividade. O objetivo deste estudo piloto foi avaliar sua eficácia como um tratamento para diarréia em 11 pacientes, todos eles portadores de síndrome do intestino irritável, com diarréia, como uma manifestação clínica. Métodos - Os pacientes foram entrevistados e um questionário foi preenchido à admissão no estudo (protocolo. Em seguida, receberam 10 mL de araruta em pó, três vezes ao dia durante um mês e descontinuaram este tratamento no mês subseqüente. Os questionários foram preenchidos após o mês de tratamento e ao fim do estudo após o mês de abstinência. Resultados - Araruta reduziu a diarréia e manteve um efeito de constipação por longo prazo. Ela também aliviou a dor abdominal. Conclusão - Araruta é um efetivo tratamento para diarr

  11. Maniobra de movilización del pedículo mesentérico superior

    Directory of Open Access Journals (Sweden)

    Alejandro García Gutiérrez

    2003-12-01

    Full Text Available Se realiza una investigación bibliográfica, con el fin de actualizar la base anátomo-embriológica y los objetivos de la maniobra de movilización del pedículo mesentérico superior en cirugía general y determinar la prioridad en su creación, basados en el análisis de los trabajos originales referidos a esta maniobra. Se concluyó que la prioridad en su empleo correspondió a los autores Petrov y Koundadzé de Rusia, quienes la emplearon para alargar la porción del intestino utilizada en las esofagoplastias, mientras que los autores norteamericanos Cattell y Braasch la realizaron para mejorar el acceso a las porciones 3ª y 4ª del duodeno y al ángulo duodeno-yeyunal, 10 años despuésA bibliographic investigation was made aimed at updating the anatomicoembriological base and the objectives of the mobilizing maneuver of the superior mesenteric pedicle in general surgery and at determining the priority in its creation, based on the analysis of the original papers referring to this maneuver. It was concluded that the priority in its use corresponded to the Russian authors Petrov and Koundadzé, who used it to enlarge the portion of the intestine utilized in the esophagoplasties, whereas the US authors Cattell and Broasch performed it to improve the access to the 3rd and 4th portions of the duodenum and to the duodeno-jejunal angle, 10 years later

  12. Ultrastructure of mouse intestinal mucosa and changes observed after long term anthraquinone administration.

    OpenAIRE

    Dufour, P; Gendre, P

    1984-01-01

    In an attempt to study the relative toxicity of anthraquinonic laxatives on intestinal mucosa, we compared in mice the effects of fruit pulp containing sennosides A and B with those of a free anthraquinone, 1-8 dihydroxyanthraquinone. Observations have been made with transmission electron microscopy (EM) after 16 weeks of treatment with the two drugs. Although the doses used in this study were equipotent in terms of laxative activity, no damage to the intestinal tissue was observed with the s...

  13. Benign Fibrous Histiocytoma of the Buccal Mucosa: Case Report and Literature Review

    Directory of Open Access Journals (Sweden)

    Paraskevi Giovani

    2010-01-01

    Full Text Available Benign fibrous histiocytoma is an interesting and challenging entity even in its most usual, cutaneous presentation. Noncutaneous presentation is extremely limited, even more so for the mucosa of the head and neck area. We herein report such a case, describing the clinical characteristics of the lesion, complete diagnostic evaluation, management, and follow-up. Diagnostic histopathological challenges are specifically illustrated. A complete review of the relevant literature is also included.

  14. Structural alterations of the mucosa stroma in the Barrett's esophagus metaplasia-dysplasia-adenocarcinoma sequence.

    Science.gov (United States)

    Bobryshev, Yuri V; Killingsworth, Murray C; Lord, Reginald V N

    2012-09-01

    Accumulating evidence suggests that the extracellular matrix play important roles in intercellular communications and contribute to the development of a number of diseases, including diseases of the gastrointestinal tract. The present study examined the structural characteristics and alterations of the extracellular matrix of the mucosa stroma in the Barrett's esophagus metaplasia-dysplasia-adenocarcinoma sequence. A total of 41 esophageal tissue specimens (15 esophageal adenocarcinoma, 10 Barrett's esophagus intestinal metaplasia, seven dysplasia and nine normal esophagus) were studied. The present study used transmission electron microscopy and computerized quantitative electron-microscopic analysis in order to investigate the characteristics of the extracellular matrix of the mucosa. The study revealed that marked structural alterations of the mucosa stroma, relating to changes in the distribution and appearance of collagen fibers as well as to changes in numbers of matrix microvesicles, occur in Barrett's esophagus and esophageal adenocarcinoma. It was found that there were 3.1 times more microvesicles in the stroma in Barrett's esophagus than in the stroma of the normal esophagus (P<0.0001) and that there were 5.8 times more microvesicles in esophageal adenocarcinoma than in the normal esophagus (P<0.0001). There were 1.9 times more microvesicles in esophageal adenocarcinoma than in Barrett's esophagus (P=0.0043). The study demonstrates distinctive alterations of the mucosa stroma extracellular matrix in the metaplasia-dysplasia-adenocarcinoma sequence. The findings suggest that the redistribution of collagen fibers and increases in numbers of matrix microvesicles may play roles in the formation of specialized intestinal metaplasia and the development of adenocarcinoma. © 2012 Journal of Gastroenterology and Hepatology Foundation and Blackwell Publishing Asia Pty Ltd.

  15. Mlh1 deficiency in normal mouse colon mucosa associates with chromosomally unstable colon cancer

    Science.gov (United States)

    Pussila, Marjaana; Törönen, Petri; Einarsdottir, Elisabet; Katayama, Shintaro; Krjutškov, Kaarel; Holm, Liisa; Kere, Juha; Peltomäki, Päivi; Mäkinen, Markus J; Linden, Jere; Nyström, Minna

    2018-01-01

    Abstract Colorectal cancer (CRC) genome is unstable and different types of instabilities, such as chromosomal instability (CIN) and microsatellite instability (MSI) are thought to reflect distinct cancer initiating mechanisms. Although 85% of sporadic CRC reveal CIN, 15% reveal mismatch repair (MMR) malfunction and MSI, the hallmarks of Lynch syndrome with inherited heterozygous germline mutations in MMR genes. Our study was designed to comprehensively follow genome-wide expression changes and their implications during colon tumorigenesis. We conducted a long-term feeding experiment in the mouse to address expression changes arising in histologically normal colonic mucosa as putative cancer preceding events, and the effect of inherited predisposition (Mlh1+/−) and Western-style diet (WD) on those. During the 21-month experiment, carcinomas developed mainly in WD-fed mice and were evenly distributed between genotypes. Unexpectedly, the heterozygote (B6.129-Mlh1tm1Rak) mice did not show MSI in their CRCs. Instead, both wildtype and heterozygote CRC mice showed a distinct mRNA expression profile and shortage of several chromosomal segregation gene-specific transcripts (Mlh1, Bub1, Mis18a, Tpx2, Rad9a, Pms2, Cenpe, Ncapd3, Odf2 and Dclre1b) in their colon mucosa, as well as an increased mitotic activity and abundant numbers of unbalanced/atypical mitoses in tumours. Our genome-wide expression profiling experiment demonstrates that cancer preceding changes are already seen in histologically normal colon mucosa and that decreased expressions of Mlh1 and other chromosomal segregation genes may form a field-defect in mucosa, which trigger MMR-proficient, chromosomally unstable CRC. PMID:29701748

  16. Prevalence of Candida spp. in Healthy Oral Mucosa Surfaces with Higher Incidence of Chronic Hyperplastic Candidosis.

    Science.gov (United States)

    Arruda, Claúdia; Artico, Gabriela; Freitas, Roseli; Filho, Antônio; Migliari, Dante

    2016-08-01

    Predisposing factors in chronic hyperplastic candidosis (CHC) have been poorly recognized. This study aimed at assessing the prevalence of Candida spp. in areas of the oral mucosa showing greater prevalent rate of CHC, such as the retrocomissural area, the lateral borders of the tongue, and the hard-palate mucosa in four groups of individuals presenting predisposing factors as follows: Smoking habits (group I); patients with low salivary flow rate (SFR) (hyposalivation - group II); patients with loss of vertical dimension of occlusion (LVDO -group III); and control subjects (group IV). A total of 44 individuals (age 4090 years, mean: 55.8 years) were divided into four groups: Group I (11 smokers); group II (10 hyposalivation patients); group III (10 LVDO patients); and group IV (control, 13 healthy subjects). All individuals were tested for Candida-pseudohyphae form by direct examination and for Candida spp. culture growth in samples obtained from the retrocomissural, tongue's lateral border, and hard-soft palatal mucosa. Direct examination showed a statistically significant prevalence rate for pseudohyphae (p < 0.05) on the retrocomissural and on tongue's lateral borders of individuals with LVDO. A statistically significant (p < 0.05) culture growth for Candida spp. was found on the retrocomissural areas of those with hyposalivation and with LVDO, and on the palate mucosa and on the tongue's lateral borders in the smokers and in the individuals with LVDO when compared with those of the control group. While direct examination is effective for detecting pseudohyphae, LVDO and tobacco smoking seem to be factors of relevance to the development of CHC. Since CHC has been linked to a high rate of malignant transformation, this study analyzes some clinical (and exogenous) factors that may contribute to the development of CHC and addresses some preventive measures to reduce its incidence.

  17. Robotic Ureteroplasty with Buccal Mucosa Graft for the Management of Complex Ureteral Strictures.

    Science.gov (United States)

    Lee, Ziho; Waldorf, Benjamin T; Cho, Eric Y; Liu, Jeffrey C; Metro, Michael J; Eun, Daniel D

    2017-12-01

    Surgical management of proximal and mid ureteral strictures that are not amenable to primary excision and anastomosis is challenging. Although a buccal mucosa graft is commonly used during substitution urethroplasty, its use in substitution ureteroplasty is limited. We describe our technique of robotic ureteroplasty with a buccal mucosa graft to manage complex ureteral strictures and we report our outcomes. We retrospectively reviewed the records of 12 patients who underwent robotic ureteroplasty with a buccal mucosa graft between September 2014 and June 2016. The indication for the procedure was a proximal or mid ureteral stricture not amenable to primary excision and anastomosis. The primary outcomes were clinical success, absent symptoms on ureteral pathology and radiological success, defined as absent ureteral obstruction on retrograde pyelography, renal scan and/or computerized tomography. Four of the 12 patients (33.3%) had a ureteropelvic junction stricture, 4 (33.3%) had a proximal stricture and 4 (33.3%) had a mid ureteral stricture. Eight of the 12 patients (66.7%) had previously undergone failed ureteral reconstruction. Median stricture length was 3 cm (range 2 to 5). Median operative time was 217 minutes (range 136 to 344) and mean estimated blood loss was 100 ml (range 50 to 200). Median length of stay was 1 day (range 1 to 6). At a median followup of 13 months (range 4 to 30) 10 of the 12 cases (83.3%) were clinically and radiologically successful. Robotic ureteroplasty with a buccal mucosa graft is associated with low inherent morbidity. It is an effective way to manage complex proximal and mid ureteral strictures. Copyright © 2017 American Urological Association Education and Research, Inc. Published by Elsevier Inc. All rights reserved.

  18. Tau Protein in Oral Mucosa and Cognitive State: A Cross-sectional Study

    Directory of Open Access Journals (Sweden)

    Luis Fernando Arredondo

    2017-10-01

    Full Text Available Neurodegenerative diseases are characterized by the presence of abnormal aggregates of proteins in brain tissue. Among them, the presence of aggregates of phosphorylated Tau protein (p-Tau is the hallmark of Alzheimer’s disease (AD and other major neurodegenerative disorders such as corticobasal degeneration and frontotemporal dementia among others. Although Tau protein has previously been assumed to be exclusive to the central nervous system, it is also found in peripheral tissues. The purpose of this study was to determine whether there is a differential Tau expression in oral mucosa cells according to cognitive impairment. Eighty-one subjects were enrolled in the study and classified per Mini-Mental State Examination test score into control, mild cognitive impairment (MCI, and severe cognitive impairment (SCI groups. Immunocytochemistry and immunofluorescence revealed the presence of Tau and four p-Tau forms in the cytoplasm and nucleus of oral mucosa cells. More positivity was present in subjects with cognitive impairment than in control subjects, both in the nucleus and cytoplasm, in a speckle pattern. The mRNA expression of Tau by quantitative real-time polymerase chain reaction was higher in SCI as compared with the control group (P < 0.01. A significantly higher percentage of immunopositive cells in the SCI group was found via flow cytometry in comparison to controls and the MCI group (P < 0.01. These findings demonstrate the higher presence of p-Tau and Tau transcript in the oral mucosa of cognitively impaired subjects when compared with healthy subjects. The feasibility of p-Tau quantification by flow cytometry supports the prospective analysis of oral mucosa as a support tool for screening of proteinopathies in cognitively impaired patients.

  19. Functional Properties of Lactobacillus mucosae Strains Isolated from Brazilian Goat Milk.

    Science.gov (United States)

    de Moraes, Georgia Maciel Dias; de Abreu, Louricélia Rodrigues; do Egito, Antônio Silvio; Salles, Hévila Oliveira; da Silva, Liana Maria Ferreira; Nero, Luís Augusto; Todorov, Svetoslav Dimitrov; Dos Santos, Karina Maria Olbrich

    2017-09-01

    The search for probiotic candidates among lactic acid bacteria (LAB) isolated from food may uncover new strains with promising health and technological properties. Lactobacillus mucosae strains attracted recent research attention due to their ability to adhere to intestinal mucus and to inhibit pathogens in the gastrointestinal tract, both related to a probiotic potential. Properties of interest and safety aspects of three Lb. mucosae strains (CNPC006, CNPC007, and CNPC009) isolated from goat milk were investigated employing in vitro tests. The presence of genetic factors related to bile salt hydrolase production (bsh), intestinal adhesion properties (msa, map, mub, and ef-tu), virulence, and biogenic amine production were also verified. All strains exhibited the target map, mub, and ef-tu sequences; the msa gene was detected in CNPC006 and CNPC007 strains. Some of the searched sequences for virulence factors were detected, especially in the CNPC009 strain; all strains carried the hyl gene, related to the production of hyaluronidase. Lb. mucosae CNPC007 exhibited a high survival rate in simulated gastric and enteric conditions. Besides, all strains exhibited the bsh sequence, and CNPC006 and CNPC007 were able to deconjugate salts of glycodeoxycholic acid (GDC). Regarding technological properties for dairy product applications, a relatively higher milk acidification and clotting capacity, diacetyl production, and proteolytic activity were registered for CNPC007 in comparison to the other strains. Collectively, the results aim at Lb. mucosae CNPC007 as a promising probiotic candidate for application in dairy products, deserving further studies to confirm and explore its potential.

  20. Urokinase and the intestinal mucosa: evidence for a role in epithelial cell turnover

    OpenAIRE

    Gibson, P; Birchall, I; Rosella, O; Albert, V; Finch, C; Barkla, D; Young, G

    1998-01-01

    Background—The functions of urokinase in intestinal epithelia are unknown. 
Aims—To determine the relation of urokinase expressed by intestinal epithelial cells to their position in the crypt-villus/surface axis and of mucosal urokinase activity to epithelial proliferative kinetics in the distal colon. 
Methods—Urokinase expression was examined immunohistochemically in human intestinal mucosa. Urokinase activity was measured colorimetrically in epithelial cells isolated sequ...

  1. 3D handheld endoscope for optical coherence tomography of the human oral mucosa in vivo

    Science.gov (United States)

    Walther, Julia; Schnabel, Christian; Ebert, Nadja; Baumann, Michael; Koch, Edmund

    2017-07-01

    The early non-invasive diagnosis of epithelial tissue alterations in daily clinical routine is still challenging. Since optical coherence tomography (OCT) shows the potential to differentiate between benign and malignant tissue of primal endothelium, OCT could be beneficial for the early diagnosis of malignancies in routine health checks. In this research, a new handheld endoscopic scanning unit was designed and connected to a spectral domain OCT system of our workgroup for the in vivo imaging of the human oral mucosa.

  2. Application of interstitial radiotherapy for cancers of the tongue and oral caving mucosa

    International Nuclear Information System (INIS)

    Zamyatin, O.A.; Vakhramova, M.P.; Razorenova, E.V.

    1997-01-01

    The report deals with new procedures of interstitial, complex and combined treatment of cancer of the tongue and mucosa of fundus of the oral cavity with time space-differentiated doses of radiation. If indicated, cervical lymph nodes undergo surgery at the final stage of therapy. Interstitial radiotherapy has proved a highly-effective radical component of said treatment for cancers of the tongue and oral cavity

  3. Effects of nonpathogenic bacteria on cytokine secretion by human intestinal mucosa.

    Science.gov (United States)

    Borruel, Natalia; Casellas, Francesc; Antolín, María; Llopis, Marta; Carol, Monica; Espíin, Eloy; Naval, Javier; Guarner, Francisco; Malagelada, Juan R

    2003-04-01

    The human intestine harbors a complex microbial ecosystem, and the mucosa is the interface between the immune system and the luminal environment. The aim of this study was to elucidate whether host-bacteria interactions influence mucosal cytokine production. Macroscopically normal colonic specimens were obtained at surgery from eight patients with neoplasm, and inflamed ileal specimens were obtained from two patients with Crohn's disease. Mucosal explants were cultured for 24 h with either nonpathogenic Escherichia coli ECOR-26, Lactobacillus casei DN-114 001, L. casei DN-114 056, L. casei ATCC-334, or Lactobacillus bulgaricus LB-10. Each study included blank wells with no bacteria. Tissue and bacteria viability were confirmed by LDH release and culture. Concentration of tumor necrosis factor (TNF)alpha, transforming growth factor beta1, interleukin (IL)-8, and IL-10 was measured in supernatants. In parallel experiments, neutralizing anti-TNFalpha antibody was added to the culture. Co-culture of mucosa with bacteria did not modify LDH release. Co-culture with L. casei strains significantly reduced TNFalpha release, whereas E. coli increased it. These effects were observed both in normal and inflamed mucosa. In combination studies, L. casei DN-114 001 prevented TNFalpha stimulation by E. coli. L. casei DN-114 001 also reduced IL-8 release via a TNFalpha-independent pathway. L. casei DN-114 056 or E. coli increased IL-10 release in the presence of neutralizing anti-TNFalpha. Nonpathogenic bacteria interact with human intestinal mucosa and can induce changes in cytokine production that are strain specific.

  4. Perineal mass protrusion with rectal mucosa: a rectal duplication that underwent exstrophy.

    Science.gov (United States)

    Sun, Junjie; Vongphet, Soulithone; Zhang, Zhichong; Mo, Jiacong

    2011-08-01

    We present a rare case of a male neonate with a perineal mass with rectal mucosa, diagnosed as an exstrophic duplication of the rectum. It was accompanied by a cord that was deeply invested in the pelvic diaphragm and was composed of smooth muscle, fibrous tissue, and some rectal glands. The association of exstrophic rectal duplication with a bifid scrotum, hypospadias, and normal anus has not been described previously in the literature. Copyright © 2011 Elsevier Inc. All rights reserved.

  5. Absence of pepsinogen A3 gene expression in the gastric mucosa of patients with gastric cancer.

    OpenAIRE

    Kuipers, E J; Peña, A S; Crusius, J B; Defize, J; van der Stoop, P; Meuwissen, S G; Pals, G

    1995-01-01

    AIMS--To investigate the expression of pepsinogen A3 (Pg3) encoding genes in the gastric mucosa of normal controls and subjects with atrophic gastritis and gastric cancer. METHODS--One hundred and fifty nine patients underwent upper gastrointestinal endoscopy with sampling of gastric biopsy specimens and serum. Pg3 isoproteins were determined by electrophoresis in serum and gastric mucosal biopsy specimens. Pg3 encoding genes were assessed by PCR in DNA obtained from peripheral blood. RESULTS...

  6. The Role of Radiotherapy in the Treatment of Gastric Mucosa-Associated Lymphoid Tissue Lymphoma

    OpenAIRE

    Nam, Taek-Keun; Ahn, Jae-Sook; Choi, Yoo-Duk; Jeong, Jae-Uk; Kim, Yong-Hyeob; Yoon, Mee Sun; Song, Ju-Young; Ahn, Sung-Ja; Chung, Woong-Ki

    2014-01-01

    Purpose To assess radiotherapy for patients with early stage gastric mucosa-associated lymphoid tissue (MALT) lymphoma with respect to survival, treatment response, and complications. Materials and Methods Enrolled into this study were 48 patients diagnosed with gastric MALT lymphoma from January 2000 to September 2012. Forty-one patients had low grade and seven had mixed component with high grade. Helicobacter pylori eradication was performed in 33 patients. Thirty-four patients received rad...

  7. The overmethylated genes in Helicobacter pylori-infected gastric mucosa are demethylated in gastric cancers

    Directory of Open Access Journals (Sweden)

    Choi Sang-Wook

    2010-11-01

    Full Text Available Abstract Background The transitional-CpG sites between weakly methylated genes and densely methylated retroelements are overmethylated in the gastric mucosa infected with Helicobacter pylori (H. pylori and they are undermethylated in the gastric cancers depending on the level of loss of heterozygosity (LOH events. This study delineated the transitional-CpG methylation patterns of CpG-island-containing and -lacking genes in view of the retroelements. Methods The transitional-CpG sites of eight CpG-island-containing genes and six CpG-island-lacking genes were semi-quantitatively examined by performing radioisotope-labelling methylation-specific PCR under stringent conditions. The level of LOH in the gastric cancers was estimated using the 40 microsatellite markers on eight cancer-associated chromosomes. Each gene was scored as overmethylated or undermethylated based on an intermediate level of transitional-CpG methylation common in the H. pylori-negative gastric mucosa. Results The eight CpG-island genes examined were overmethylated depending on the proximity to the nearest retroelement in the H. pylori-positive gastric mucosa. The six CpG-island-lacking genes were similarly methylated in the H. pylori-positive and -negative gastric mucosa. In the gastric cancers, long transitional-CpG segments of the CpG-island genes distant from the retroelements remained overmethylated, whereas the overmethylation of short transitional-CpG segments close to the retroelements was not significant. Both the CpG-island-containing and -lacking genes tended to be decreasingly methylated in a LOH-level-dependent manner. Conclusions The overmethylated genes under the influence of retroelement methylation in the H. pylori-infected stomach are demethylated in the gastric cancers influenced by LOH.

  8. Bleeding peptic ulcer caused by ectopic gastric mucosa in a duplicated segment of jejunum

    International Nuclear Information System (INIS)

    Newmark, H.; Ching, G.; Halls, J.; Levy, I.J.

    1981-01-01

    The authors present a case in which a patient suffered a bleeding jejunal ulcer caused by heterotopic gastric mucosa in a congenital duplication of a segment of jejunum. This is the first case diagnosed preoperatively by two different radiographic means. These lesions were shown by both pertechnetate flow and barium small bowel studies. The rarity of these entities and the modalities used for diagnosis are described

  9. Mapping Local Cytosolic Enzymatic Activity in Human Esophageal Mucosa with Porous Silicon Nanoneedles.

    Science.gov (United States)

    Chiappini, Ciro; Campagnolo, Paola; Almeida, Carina S; Abbassi-Ghadi, Nima; Chow, Lesley W; Hanna, George B; Stevens, Molly M

    2015-09-16

    Porous silicon nanoneedles can map Cathepsin B activity across normal and tumor human esophageal mucosa. Assembling a peptide-based Cathepsin B cleavable sensor over a large array of nano-needles allows the discrimination of cancer cells from healthy ones in mixed culture. The same sensor applied to tissue can map Cathepsin B activity with high resolution across the tumor margin area of esophageal adenocarcinoma. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  10. Efeito genotóxico do etanol em células da mucosa bucal

    Directory of Open Access Journals (Sweden)

    Reis Silvia Regina de Almeida

    2002-01-01

    Full Text Available O etanol é um dos agentes químicos relacionados ao desenvolvimento de neoplasias malignas bucais. Os micronúcleos são porções de cromatina que permanecem próximas ao núcleo, resultantes de mitoses aberrantes após a ação de agentes genotóxicos. Dessa forma, sua ocorrência reflete o grau de exposição celular a carcinógenos. O objetivo deste trabalho foi avaliar a freqüência de micronúcleos em células esfoliadas da língua e da mucosa jugal de indivíduos dependentes químicos de etanol. A amostra constou de células esfoliadas da língua e da mucosa jugal de 40 indivíduos alcoólatras não fumantes e de 20 abstêmios de álcool e fumo. As células obtidas foram coradas pelo método de Feulgen e contracoradas pelo "Fast Green". Observou-se um aumento estatisticamente significativo da freqüência de micronúcleos em células esfoliadas da língua no grupo de indivíduos expostos ao etanol em relação ao grupo controle (p 0,05. Conclui-se, portanto, que o consumo excessivo de etanol promove alterações efetivas em células da mucosa bucal, mesmo na ausência de exposição ao fumo. Tais alterações apresentam-se mais expressivas no bordo lateral de língua, um sítio mais exposto à ação de carcinógenos quando comparado à mucosa jugal.

  11. Gene methylation profiles of normal mucosa, and benign and malignant colorectal tumors identify early onset markers

    Directory of Open Access Journals (Sweden)

    Vatn Morten

    2008-12-01

    Full Text Available Abstract Background Multiple epigenetic and genetic changes have been reported in colorectal tumors, but few of these have clinical impact. This study aims to pinpoint epigenetic markers that can discriminate between non-malignant and malignant tissue from the large bowel, i.e. markers with diagnostic potential. The methylation status of eleven genes (ADAMTS1, CDKN2A, CRABP1, HOXA9, MAL, MGMT, MLH1, NR3C1, PTEN, RUNX3, and SCGB3A1 was determined in 154 tissue samples including normal mucosa, adenomas, and carcinomas of the colorectum. The gene-specific and widespread methylation status among the carcinomas was related to patient gender and age, and microsatellite instability status. Possible CIMP tumors were identified by comparing the methylation profile with microsatellite instability (MSI, BRAF-, KRAS-, and TP53 mutation status. Results The mean number of methylated genes per sample was 0.4 in normal colon mucosa from tumor-free individuals, 1.2 in mucosa from cancerous bowels, 2.2 in adenomas, and 3.9 in carcinomas. Widespread methylation was found in both adenomas and carcinomas. The promoters of ADAMTS1, MAL, and MGMT were frequently methylated in benign samples as well as in malignant tumors, independent of microsatellite instability. In contrast, normal mucosa samples taken from bowels without tumor were rarely methylated for the same genes. Hypermethylated CRABP1, MLH1, NR3C1, RUNX3, and SCGB3A1 were shown to be identifiers of carcinomas with microsatellite instability. In agreement with the CIMP concept, MSI and mutated BRAF were associated with samples harboring hypermethylation of several target genes. Conclusion Methylated ADAMTS1, MGMT, and MAL are suitable as markers for early tumor detection.

  12. The state of teeth and oral mucosa of children from the radionuclide contaminated districts

    International Nuclear Information System (INIS)

    Mel'nichenko, Eh.M.; Gorbacheva, K.A.

    1994-01-01

    In 1986-1987 the stomatology inspection of 862 children in the age of 6-14 years, living at the territory with the level of contamination from 20 to 40 Ci/km 2 has been carried out. High teeth caries disease, bad mouth hygiene, oral mucosa and red lips margin changes was revealed. The teeth caries disease did not depend on the level of thr resident radioactive contamination. 7 refs., 2 tabs

  13. Cambios en las características estructurales y reológicas de pastas libres de gluten durante el proceso de cocción

    OpenAIRE

    Larrosa, Virginia Judit; Lorenzo, Gabriel; Zaritzky, Noemí E.; Califano, Alicia

    2013-01-01

    La enfermedad celíaca consiste en una intolerancia irreversible a la fracción gliadina de las proteínas de trigo, y a las prolaminas de centeno, cebada y avena. En Argentina se estima que uno de cada 100 individuos padece de celiaquía. Esta situación condiciona, en pacientes, a desarrollar lesiones severas de la mucosa del intestino delgado. El único tratamiento efectivo que tienen los pacientes es ajustarse a una dieta libre de gluten durante toda su vida. En los últimos años ha aumentado...

  14. Evaluación de la validez y fiabilidad de un cuestionario específico de calidad de vida relacionada con la salud en celíacos de 8-18 años.

    OpenAIRE

    Barrio Torres, Josefa

    2016-01-01

    Tesis Doctoral leída en la Universidad Rey Juan Carlos de Madrid en 2015. Directores de la Tesis: Angel Gil Miguel, Enriqueta Román Riechmann y Mª Luz Cilleruelo La enfermedad celíaca (EC) es una enfermedad sistémica mediada por el sistema inmune y ocasionada por el gluten y las prolaminas relacionadas que se produce en individuos genéticamente predispuestos. Cursa con una atrofia de las vellosidades intestinales de la mucosa del intestino delgado superior y como consecuencia s...

  15. Profilicollis altmani (Perry, 1942 Van Cleave, 1947 (Acanthocephala en el Perú. Con notas sobre la infección experimental de mamiferos terrestres

    Directory of Open Access Journals (Sweden)

    Manuel Tantaleán

    2013-06-01

    Full Text Available Entre los meses de enero y febrero de 2001, se hizo el estudio parasitológico de Emerita analoga Stimpson (Hippidae procedentes de la playa Bujama (Mala, Lima, encontrando que de 37 a 48% de ellos estaban infectados con cistacantos identificados como de Profilicollis altmani (Perry, 1942 Van Cleave, 1947. Estos cistacantos se inocularon en ratas, ratones, hamsters y un cachorro de perro, que resultaron todos infectados. Los parásitos se localizaron principalmente en la cavidad abdominal y/o adheridos a la mucosa del intestino delgado y grueso. El hombre puede infectarse con estos parásitos al ingerir E. analoga infectados.

  16. Adenocarcinoma of the cervical esophagus arising in heterotopic gastric mucosa: exclusive chemoradiotherapy following a mucosal resection

    International Nuclear Information System (INIS)

    Jestin-Letallec, V.; Muller, M.; Metges, J.P.; Bouchekoua, M.; Albarghach, N.; Pradier, O.

    2007-01-01

    Esophagus adenocarcinomas developing within heterotopic gastric mucosa are very rare and described to be found endoscopically in a prevalence of .29%. We report a case of cervical adenocarcinoma arising in ectopic gastric mucosa in a fifty-four year old man. The patient underwent a mucosal resection followed with exclusive chemoradiotherapy because of infiltration of the sub mucosa layer. A radiotherapy dose of 60 Gy ( 2 Gy/Fr, 30 Fr) was realized with a reduction of the fields at 50 Gy associated with a continuous 5FU-cisplatin combination after eliminating known mutation in the dihydro-pyrimidine of the dehydrogenase gene. for this tumor, surgery is the main treatment, (oesophagectomy associated with laryngo-pharyngectomy) and has an important repercussion on the quality of life. Because of the refusal of our patient, after a mucosal resection attempt, we proposed our patient a chemoradiotherapy. For the first time in the literature, we report the results of radio chemotherapy for this rare tumor. Eighteen months after the treatment, the patient is alive without sign of recurrence. The radio chemotherapy could be a safety treatment for this rare tumor associated with a good quality of life. A review of the literature since 1950 will be shown. (authors)

  17. Adenocarcinoma of the cervical esophagus arising in heterotopic gastric mucosa: exclusive chemoradiotherapy following a mucosal resection

    Energy Technology Data Exchange (ETDEWEB)

    Jestin-Letallec, V.; Muller, M.; Metges, J.P.; Bouchekoua, M.; Albarghach, N.; Pradier, O. [Departement de Cancerologie, 29 - Brest (France)

    2007-11-15

    Esophagus adenocarcinomas developing within heterotopic gastric mucosa are very rare and described to be found endoscopically in a prevalence of .29%. We report a case of cervical adenocarcinoma arising in ectopic gastric mucosa in a fifty-four year old man. The patient underwent a mucosal resection followed with exclusive chemoradiotherapy because of infiltration of the sub mucosa layer. A radiotherapy dose of 60 Gy ( 2 Gy/Fr, 30 Fr) was realized with a reduction of the fields at 50 Gy associated with a continuous 5FU-cisplatin combination after eliminating known mutation in the dihydro-pyrimidine of the dehydrogenase gene. for this tumor, surgery is the main treatment, (oesophagectomy associated with laryngo-pharyngectomy) and has an important repercussion on the quality of life. Because of the refusal of our patient, after a mucosal resection attempt, we proposed our patient a chemoradiotherapy. For the first time in the literature, we report the results of radio chemotherapy for this rare tumor. Eighteen months after the treatment, the patient is alive without sign of recurrence. The radio chemotherapy could be a safety treatment for this rare tumor associated with a good quality of life. A review of the literature since 1950 will be shown. (authors)

  18. Vasoactive intestinal peptide and somatostatin in the plasma and sigmoid mucosa in irritable bowel syndrome

    International Nuclear Information System (INIS)

    Zhang Ru; Wang Fuxian

    2004-01-01

    To investigate the possible role and clinical significance of vasoactive intestinal peptide (VIP) and somatostatin(SS) in the irritable bowel syndrome (IBS), the VIP and SS in the plasma and sigmoid mucosa were measured by radioimmunoassay in the control group and the IBS group. The VIP concentration in the plasma and sigmoid mucosa of the IBS patients with constipation was significantly higher than that of the control group (P<0.01), while that of the IBS patients with diarrhea was significantly lower than that of the control group (P<0.05). The SS concentration in two sites was significantly elevated in IBS patients of both types and was significantly higher in IBS with constipation than in IBS with diarrhea (P<0.05). Conclusion: The VIP and SS in IBS are abnormal, which might play a role in the pathogenesis of IBS. The plasma and mucosa concenration of VIP and SS in two kinds of IBS patients are significantly different, which indicates that there might be different pathophysiological basis involved in the pathogenesis of the two kinds of IBS patients. (authors)

  19. Evaluation of tissue engineered models of the oral mucosa to investigate oral candidiasis.

    Science.gov (United States)

    Yadev, Nishant P; Murdoch, Craig; Saville, Stephen P; Thornhill, Martin H

    2011-06-01

    Candida albicans is a commensal organism that can be isolated from the majority of healthy individuals. However, in certain susceptible individuals C. albicans can become pathogenic leading to the mucocutaneous infection; oral candidiasis. Murine models and in vitro monolayer cultures have generated some data on the likely virulence and host factors that contribute to oral candidiasis but these models have limitations. Recently, tissue engineered oral mucosal models have been developed to mimic the normal oral mucosa but little information is available on their true representation. In this study, we assessed the histological features of three different tissue engineered oral mucosal models compared to the normal oral mucosa and analysed both cell damage and cytokine release following infection with C. albicans. Models comprised of normal oral keratinocytes and a fibroblast-containing matrix displayed more similar immunohistological and proliferation characteristics to normal mucosa, compared to models composed of an oral carcinoma cell line. Although all models were invaded and damaged by C. albicans in a similar manner, the cytokine response was much more pronounced in models containing normal keratinocytes. These data suggest that models based on normal keratinocytes atop a fibroblast-containing connective tissue will significantly aid in dissecting the molecular pathogenesis of oral candidiasis. Copyright © 2011 Elsevier Ltd. All rights reserved.

  20. Squamous cell carcinoma of buccal mucosa: An analysis of prognostic factors

    Directory of Open Access Journals (Sweden)

    Saurabh Bobdey

    2018-01-01

    Full Text Available Introduction: Carcinoma of the buccal mucosa is the most common oral cavity cancer in the Indian subcontinent. The aim of this study was to analyze the outcome and evaluate prognostic factors in surgically treated buccal mucosa squamous cell carcinoma (BMSCC patients. Materials and Methods: A retrospective study was performed by reviewing the medical records of 409 pathologically proven buccal mucosa cancer patients, who were diagnosed and surgically treated in Tata Memorial Hospital between January 1, 2006, and December 31, 2008. Results: The overall 5-year survival of the cohort was found to be 54.1%. The stage-wise survival rate for tumor, node, metastasis (TNM Stage I, II, III, and IV patients was found to be 85.2%, 82.9%, 56.3%, and 42.6% (P < 0.00, respectively. On multivariate Cox proportional hazard analysis, the presence of comorbidity, histological tumor size, pathological lymph node status, tumor differentiation, perineural invasion, and extracapsular spread were found to be independently associated with overall survival. Conclusion: BMSCC is an aggressive malignant tumor. In addition to TNM classification, other clinical and pathological factors also have a significant role in BMSCC prognostication. Hence, there is a need to move beyond TNM and develop a more inclusive, flexible, and easy to use prognostic system.

  1. Outcome of buccal mucosa urethroplasty in the management of urethral strictures

    Directory of Open Access Journals (Sweden)

    Basri Cakiroglu

    2017-06-01

    Full Text Available Objective: The objective of the study is to report the outcome of buccal mucosal urethroplasty. Materials and methods: The follow up data of 15 patients undergoing single stage urethroplasty from September 2010 to September 2015 were retropectively reviewed. They received buccal mucosa graft for urethroplasty. The patients were followed for complications and outcome. Results: Mean age was 53.7 ± 13.6 The stricture length ranged from 3 to 6 cm (mean 4.4 ± 0.8. The success rate for buccal mucosa urethroplasty (BMU was 67.7% at 12th month. Three patients presenting with voiding difficulty in the 3rd month and one in the next 12 months, had urethral restenosis. One patient had fistula formation at 6th month postoperatively. Five patients underwent retreatment procedures such as internal urethrotomy, urethroplasty and/or internal urethrotomy. Conclusions: The buccal mucosa is easy to obtain and handle, therefore BMU can be safely and effectively managed outside high volume institutions.

  2. Angiogenesis in the reparatory mucosa of the mandibular edentulous ridge is driven by endothelial tip cells.

    Science.gov (United States)

    Stănescu, Ruxandra; Didilescu, Andreea Cristiana; Jianu, Adelina Maria; Rusu, M C

    2012-01-01

    Sprouting angiogenesis is led by specialized cell--the endothelial tip cells (ETCs) which can be targeted by pro- or anti-angiogenic therapies. We aimed to perform a qualitative study in order to assess the guidance by tip cells of the endothelial sprouts in the repairing mucosa of the edentulous mandibular crest. Mucosa of the mandibular edentulous ridge was collected from six adult patients, prior to healing abutment placement (second surgery). Slides were prepared and immunostained with antibodies for CD34 and Ki67. The abundant vasculature of the lamina propria was observed on slides and the CD34 antibodies labeled endothelial tip cells in various stages of the endothelial sprouts. Ki67 identified positive endothelial cells, confirming the proliferative status of the microvascular bed. According to the results, the in situ sprouting angiogenesis is driven by tip cells in the oral mucosa of the edentulous ridge and these cells can be targeted by various therapies, as required by the local pathologic or therapeutic conditions.

  3. Association of mast cells with helicobacter pylori infection in the antral mucosa

    Directory of Open Access Journals (Sweden)

    SR KC

    2011-03-01

    Full Text Available Background: Helicobacter pylori infection is associated with mixed inflammatory cell infiltrate consisting of neutrophils, eosinophils, lymphocytes and plasma cells. Helicobacter pylori lead to mast cell degranulation and release of active chemical compounds in in-vitro conditions. The objective of this study was to find out the association of mast cell density and Helicobacter pylori in the antral mucosa of the stomach. Materials and Methods: A total of 150 endoscopic biopsies were included in the study. In addition to routine Hematoxylin and Eosin stained slides, Giemsa stain was done in each case for the evaluation of Helicobacter pylori and mast cell density in the gastric mucosa. Results: Out of 150 gastric biopsies with histopathological diagnosis of chronic gastritis, 36 cases (24% were positive for Helicobacter pylori. In the antral mucosa, mast cell density was significantly higher in the Helicobacter pylori-positive group than in the Helicobacter pylori-negative group (P<0.01. Conclusion: Mast cells may play a role in the development of Helicobacter pylori gastritis. Keywords: Gastritis; Mast Cell; Helicobacter pylori DOI: 10.3126/jpn.v1i1.4448 Journal of Pathology of Nepal (2011 Vol.1, 34-36

  4. In vivo microscopic imaging of the bronchial mucosa using an endo-cytoscopy system.

    Science.gov (United States)

    Shibuya, Kiyoshi; Fujiwara, Taiki; Yasufuku, Kazuhiro; Alaa, Mohamed; Chiyo, Masako; Nakajima, Takahiro; Hoshino, Hidehisa; Hiroshima, Kenzo; Nakatani, Yukio; Yoshino, Ichiro

    2011-05-01

    We investigated the capabilities of an endo-cytoscopy system (ECS) that enables microscopic imaging of the tracheobronchial tree during bronchoscopy, including normal bronchial epithelium, dysplastic mucosa and squamous cell carcinoma. The newly developed ECS has a 3.2 mm diameter that can be passed through the 4.2 mm working channel of a mother endoscope for insertion of the ECS. It has a high magnification of 570× on a 17 in. video monitor. Twenty-two patients (7 squamous cell carcinoma, 11 squamous dysplasia and 4 after PDT therapies) were underwent white light, NBI light and AFI bronchoscopy. Both abnormal areas of interest and normal bronchial mucosa were stained with 0.5% methylene blue and examined with ECS at high magnification (570×). Histological examinations using haematoxylin and eosin staining were made of biopsied specimens. Analyzed ECS images were compared with the corresponding histological examinations. In normal bronchial mucosa, ciliated columnar epithelial cells were visible. In bronchial squamous dysplasia, superficial cells with abundant cytoplasm were arranged regularly. In squamous cell carcinoma, large, polymorphic tumor cells showed increased cellular densities with irregular stratified patterns. These ECS images corresponded well with the light-microscopic examination of conventional histology. ECS was useful for the discrimination between normal bronchial epithelial cells and dysplastic cells or malignant cells during bronchoscopy in real time. This novel technology has an excellent potential to provide in vivo diagnosis during bronchoscopic examinations. Copyright © 2010 Elsevier Ireland Ltd. All rights reserved.

  5. Transepithelial transport of PAMAM dendrimers across isolated rat jejunal mucosae in ussing chambers.

    Science.gov (United States)

    Hubbard, Dallin; Ghandehari, Hamidreza; Brayden, David J

    2014-08-11

    Oral delivery remains a challenge for poorly permeable hydrophilic macromolecules. Poly(amido amine) (PAMAM) dendrimers have shown potential for their possible oral delivery. Transepithelial transport of carboxyl-terminated G3.5 and amine-terminated G4 PAMAM dendrimers was assessed using isolated rat jejunal mucosae mounted in Ussing chambers. The 1 mM FITC-labeled dendrimers were added to the apical side of mucosae. Apparent permeability coefficients (Papp) from the apical to the basolateral side were significantly increased for FITC when conjugated to G3.5 PAMAM dendrimer compared to FITC alone. Minimal signs of toxicity were observed when mucosae were exposed to both dendrimers with respect to transepithelial electrical resistance changes, carbachol-induced short circuit current stimulation, and histological changes. [(14)C]-mannitol fluxes were not altered in the presence of 1 mM dendrimers, suggesting that the paracellular pathway was not affected at this concentration in this model. These results give insight into the mechanism of PAMAM dendrimer transepithelial rat jejunal transport, as well as toxicological considerations important for oral drug delivery.

  6. Bacteria and Candida yeasts in inflammations of the oral mucosa in children with secondary immunodeficiency.

    Science.gov (United States)

    Olczak-Kowalczyk, Dorota; Daszkiewicz, Marta; Krasuska-Sławińska; Dembowska-Bagińska, Bozena; Gozdowski, Dariusz; Daszkiewicz, Paweł; Fronc, Beata; Semczuk, Katarzyna

    2012-08-01

    Oral microbial flora and a damaged oral mucosa may increase the risk of bacteriemia, fungemia and complications in immunocompromised patients. Assessment of presence: bacteria and Candida spp. in different oral lesions, and the incidence of bacteremia in the case of a damaged mucosa in transplant recipients and patients receiving anti-tumour chemotherapy. Forty-five patients – 18 months to 18 years of life, were included (20 – organ recipients, 14– anti-tumour chemotherapy, 11 – control group). Clinical, oral mucosa examination focused on the type, severity and site of lesions, and microbiology assessed the presence of bacteria and fungi in the material from lesions. Blood cultures were performed in ten immunocompromised patients with manifestations of systemic infection. The control material consisted of blood cultures made prior to the onset of oral lesions and after 4–6 weeks following their remission in a diagnosed bacteremia. The statistical analysis was performed. In the subjects with secondary immunodeficiency, among other coagulase-negative Staphylococcus (CoNS), Candidia spp. were more frequent. In cancer patients, mucositis was associated with Candida spp., Streptococcus spp. Organ recipients with stomatitis exhibited the presence of CoNS, Streptococcus viridians and other. Oral lesions in the control group contained Haemophilus parainfluenzae, Neisseria spp. and Staphylococcus aureus. In 30% of immunocompromised patients, oral lesions were accompanied by bacteremia. A correlation has been found between oral lesions and the presence of S. aureus in patients without secondary immunodeficiency, and of CoNS, Enterococcus spp., Candida spp. in immunocompromised patients.

  7. Cross-sample validation provides enhanced proteome coverage in rat vocal fold mucosa.

    Directory of Open Access Journals (Sweden)

    Nathan V Welham

    2011-03-01

    Full Text Available The vocal fold mucosa is a biomechanically unique tissue comprised of a densely cellular epithelium, superficial to an extracellular matrix (ECM-rich lamina propria. Such ECM-rich tissues are challenging to analyze using proteomic assays, primarily due to extensive crosslinking and glycosylation of the majority of high M(r ECM proteins. In this study, we implemented an LC-MS/MS-based strategy to characterize the rat vocal fold mucosa proteome. Our sample preparation protocol successfully solubilized both proteins and certain high M(r glycoconjugates and resulted in the identification of hundreds of mucosal proteins. A straightforward approach to the treatment of protein identifications attributed to single peptide hits allowed the retention of potentially important low abundance identifications (validated by a cross-sample match and de novo interpretation of relevant spectra while still eliminating potentially spurious identifications (global single peptide hits with no cross-sample match. The resulting vocal fold mucosa proteome was characterized by a wide range of cellular and extracellular proteins spanning 12 functional categories.

  8. Morphological and morphometric characteristics of gastric mucosa in western grey kangaroo (Macropus fuliginosus

    Directory of Open Access Journals (Sweden)

    Mahmoud Badran Shoeib

    2015-03-01

    Full Text Available The present study was aimed to investigate the morphology and histomorphometry of stomach and gastric mucosa in western grey kangaroo (Macropus fuliginosus. The stomach was composed of three indistinctive separate parts namely sacciform forestomach, tubiform forestomach, and hindstomach. The tubiform forestomach was the main tubular section of the organ. The stomach had a compound lining. The non-glandular mucosa occupied the medial blind sac (MBS of the sacciform forestomach; the layer covered about one-third of the tubiform forestomach (non-glandular region and the entire length of the gastric sulcus. The glandular part lined the parietal blind sac (PBS of sacciform forestomach and the cardiac gland region of tubiform forestomach as well as fundic and pyloric gland regions of the hindstomach. The cardiac mucosa had smooth and folded areas; these were filled with mixed glands. In the fundic glands, the parietal cells outnumbered the chief cells. The pyloric glands were of serous-like in characteristics. In conclusion, gross and histological structures of the stomach of western grey kangaroo are adaptive with its food habitat, which allows thorough mixing of highly fibrous grasses.

  9. DNA damage in oral mucosa cells of patients with fixed orthodontic appliances.

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    Farzin Heravi

    2013-12-01

    Full Text Available The release of toxic metal ions from orthodontic alloys has induced concerns regarding the biocompatibility of fixed appliances. This study investigated the genotoxic effect of metal appliances in a sample of patients undergoing fixed orthodontic treatment.The study included twenty-five healthy individuals requiring orthodontic therapy in both jaws. The patients were treated by stainless steel orthodontic brackets and nickel-titanium or stainless steel arch wires. The oral mucosa cells were gathered just before the appliance placement and 9 months later. The cells were centrifuged, fixed and dropped onto slides. After staining, the micronucleus (MN assay was used to determine genome alteration. The data were analyzed by paired sample t-test.The mean micronuclei frequency in the buccal mucosa was 10.6 ± 5.7 per 1000 cells before the appliance placement and 9.2 ± 6.37 per 1000 cells 9 months later. No significant difference was found in the MN count before and 9 months after therapy (p=0.336.Under the conditions used in this study, application of fixed orthodontic appliances did not expose healthy individuals to increased risk of DNA damage in oral mucosa cells.

  10. Antimicrobial activity of Anonna mucosa (Jacq. grown in vivo and obtained by in vitroculture

    Directory of Open Access Journals (Sweden)

    Thiago José de Souza Barboza

    2015-09-01

    Full Text Available Brazilian flora includes numerous species of medicinal importance that can be used to develop new drugs. Plant tissue culture offers strategies for conservation and use of these species allowing continuous production of plants and bioactive substances. Annona mucosa has produced substances such as acetogenins and alkaloids that exhibit antimicrobial activities. The widespread use of antibiotics has led to an increase in multidrug-resistant bacteria, which represents a serious risk of infection. In view of this problem, the aim of this work was to evaluate the antibacterial potential of extracts of A. mucosa obtained by in vitro techniques and also cultured under in vivo conditions. Segments from seedlings were inoculated onto different culture media containing the auxin picloram and the cytokinin kinetin at different concentrations. The calluses obtained were used to produce cell suspension cultures. The materials were subjected to methanol extraction and subsequent fractionation in hexane and dichloromethane. The antimicrobial activity against 20 strains of clinical relevance was evaluated by the macrodilution method at minimum inhibitory and minimum bactericidal concentrations. The extracts showed selective antimicrobial activity, inhibiting the growth of Streptococcus pyogenes and Bacillus thuringiensis at different concentrations. The plant tissue culture methods produced plant materials with antibacterial properties, as well as in vivo grown plants. The antibacterial activity of material obtained through biotechnological procedures of A. mucosa is reported here for the first time.

  11. Bone marrow transplantation rescues intestinal mucosa after whole body radiation via paracrine mechanisms

    International Nuclear Information System (INIS)

    Chang, Ya Hui; Lin, Li-Mei; Lou, Chi-Wen; Chou, Chuan-Kai; Ch’ang, Hui-Ju

    2012-01-01

    Purpose: Our previous study reveals bone marrow transplantation (BMT) recruits host marrow-derived myelomonocytic cells to radiation-injured intestine, enhancing stromal proliferation, leading secondarily to epithelial regeneration. In this study, we propose BMT ameliorates intestinal damage via paracrine mechanisms. Materials and methods: Angiogenic cytokines within the intestinal mucosa of mice after whole body irradiation (WBI) with or without BMT were measured by cytokine array and ELISA. BM conditioned medium (BMCM) with or without treatment with neutralizing antibodies to angiogenic cytokines were continuously infused into mice for three days after radiation. Carrageenan was used to deplete myelomonocytic cells of mice. Results: BMT increased VEGF, bFGF and other angiogenic and chemotactic cytokines in the intestinal mucosa within 24 h after WBI. Infusion of BMCM ameliorated radiation-induced intestinal damage with improved stromal activity and prolonged survival of mice. Neutralization of bFGF, PDGF and other angiogenic cytokines within BMCM abolished the mitigating effect to the intestine. Pretreatment of carrageenan to recipient mice reversed some of the cytokine levels, including VEGF, bFGF and IGF within the intestinal mucosa after BMT. Conclusions: Our result suggests BMT recruits host myelomonocytic cells and enhances intestinal stroma proliferation after radiation by secreting cytokines enhancing angiogenesis and chemotaxis. Host myelomonocytic cells further uplift the paracrine effect to enhance intestinal mucosal recovery.

  12. Recovery time for inflamed middle ear mucosa in chronic otitis media.

    Science.gov (United States)

    Pakır, Onur; Dinç, Aykut Erdem; Damar, Murat; Akyıldız, İlker; Eliçora, Sultan Şevik; Erdem, Duygu

    2016-01-01

    The present study shows that 2-3 weeks after medical treatment the status of middle ear mucosa in draining ears is similar to that of dry ears for at least 3 months. To measure the time required for an inflamed middle ear mucosa to return into optimal state after appropriate medical treatment in chronic suppurative otitis media (CSOM). To assess optimal timing for elective surgical treatment of draining ears in uncomplicated CSOM. In this prospective study, the Eustachian tube (ET) mucociliary clearance time (MCT) was used as the method to demonstrate the status of middle ear mucosa. In group 1 (28 patients) ET-MCT was measured in ears that were free of drainage for at least 3 months. In Group 2 (21 patients), ET-MCT was measured in draining ears, who responded to 10-14 days medical treatment, at presentation, after 10 days and 1 month. The ET-MCT was 8.63 ± 1.32 min in group 1 and 28.96 ± 8.19 min in group 2 at presentation; and the difference was statistically significant (p < 0.001). The ET-MCT was 14.76 ± 5.11 min after 10 days and 9.31 ± 2.33 min after 1 month in group 2. The ET-MCT was indifferent between groups 1 and 2 after 1 month (p = 0.235).

  13. Freud: enfermedades nerviosas, angustia y estrés. O del estatuto del cuerpo implicado en las dolencias del sujeto. // Freud: nervous system diseases, anxiety and stress. Or of the status of the body involved in subject’s ailments

    Directory of Open Access Journals (Sweden)

    Gloria Gómez

    2008-12-01

    Full Text Available What was the destiny of the so-called nervous system diseases in times of Freud? ¿What role do they play in current nosography? New terms are coined for old conditions: stress, irritable bowel syndrome, fibromyalgia, chronic fatigue… Since Freud, the psychoanalytic clinic is made of what causes an impasse to medicine, whose idiopathic symptoms disturb the organ systems and, particularly, the bodily functions in their two orders: Vegetative life, and animal or relationship life. The body of the subject represents a problem for medical practice since this latter focuses on its dysfunctions and diseases but faces up to a body occupied by a speaking-being, a body that beyond being just a living being is a body molded by language: body of a desiring and enjoying subject. // ¿Cuál fue el destino de las llamadas enfermedades nerviosas en la época de Freud? ¿Qué lugar ocupan ellas en la nosografía actual? Se acuñan nuevos términos para viejas afecciones: estrés, síndrome de intestino irritable, fibromialgia, fatiga crónica… Desde Freud la clínica psicoanalítica del cuerpo está hecha con aquello que hace impasse a la medicina, cuyos cuadros idiopáticos perturban los sistemas orgánicos y, sobre todo, las funciones corporales en sus dos órdenes: vida vegetativa y vida animal o de relación. El cuerpo del sujeto constituye un problema para la práctica médica que apunta a sus disfunciones y enfermedades pero se confronta con un cuerpo habitado por un ser-hablante, un cuerpo que más allá de ser sólo organismo viviente, es cuerpo moldeado por el lenguaje: cuerpo de un sujeto de deseo y goce.

  14. Significant Depletion of CD4+ T Cells Occurs in the Oral Mucosa during Simian Immunodeficiency Virus Infection with the Infected CD4+ T Cell Reservoir Continuing to Persist in the Oral Mucosa during Antiretroviral Therapy

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    Jeffy George

    2015-01-01

    Full Text Available Human and simian immunodeficiency virus (HIV and SIV infections are characterized by manifestation of numerous opportunistic infections and inflammatory conditions in the oral mucosa. The loss of CD4+ T cells that play a critical role in maintaining mucosal immunity likely contributes to this process. Here we show that CD4+ T cells constitute a minor population of T cells in the oral mucosa and display a predominantly central memory phenotype mirroring other mucosal sites such as the rectal mucosa. Chronic SIV infection was associated with a near total depletion of CD4+ T cells in the oral mucosa that appear to repopulate during antiretroviral therapy (ART. Repopulating CD4+ T cells harbored a large fraction of Th17 cells suggesting that ART potentially reconstitutes oral mucosal immunity. However, a minor fraction of repopulating CD4+ T cells harbored SIV DNA suggesting that the viral reservoir continues to persist in the oral mucosa during ART. Therapeutic approaches aimed at obtaining sustainable CD4+ T cell repopulation in combination with strategies that can eradicate the latent viral reservoir in the oral mucosa are essential for better oral health and long-term outcome in HIV infected patients.

  15. Uso del microhábitat por el protozoo parásito Aggregata patagonica Sardella, Ré & Timi, 2000 (Apicomplexa: Aggregatidae en su hospedador definitivo, el pulpo Enteroctopus megalocyathus (Gould, 1852 (Cephalopoda: Octopodidae en el sur de Chile Microhabitat use by the protozoan parasite Aggregata patagonica Sardella, Ré & Timi, 2000 (Apicomplexa: Aggregatidae in his definitive host Enteroctopus megalocyathus (Gould, 1852 (Cephalopoda: Octopodidae in southern Chile

    Directory of Open Access Journals (Sweden)

    CHRISTIAN M. IBÁÑEZ

    2005-09-01

    Full Text Available Los protozoos de la familia Aggregatidae requieren de dos hospedadores para completar su ciclo biológico: un crustáceo y un cefalópodo. En este estudio se busca evidenciar si existe un uso diferencial de microhábitats de las infrapoblaciones de Aggregata patagonica entre dos zonas del tracto digestivo de su hospedador definitivo, el pulpo Enteroctopus megalocyathus. Para ello, se examinaron 40 ejemplares de Ancud y 37 de Quellón, provenientes de las capturas artesanales de la Isla de Chiloé. El tamaño y la densidad de los ooquistes se cuantificaron en el ciego y el intestino del hospedador. Además, se efectuaron observaciones histológicas para determinar cuantitativamente la ocurrencia de los distintos estados de desarrollo del parásito. En Ancud el 80 % de los pulpos estaban parasitados en el ciego y el intestino, mientras que en Quellón era el 100 %. Se determinaron cuatro estados de desarrollo (microgametos, cigotos, esporoquistes inmaduros y maduros. Los ooquistes de A. patagonica fueron más pequeños y abundantes en el ciego, pero la cobertura fue mayor en el intestino. Además, la densidad y cobertura aumenta en pocos meses entre una muestra y otra. Al parecer, A. patagonica no usaría a los dos microhábitats como lugares de preferencia para pasar los diferentes estados de desarrollo, sino más bien, y según las condiciones del microhábitat, en el intestino crecerían más los ooquistes pero en menos cantidad, ocupando una mayor superficie del microhábitat, contrario a lo que ocurre en el ciego. Por esto se sugiere que A. patagonica hace un uso diferencial del microhábitat, mediante dos estrategias distintas de historia de vidaThe protozoan parasites of the family Aggregatidae, require two hosts to complete their life cycle, a crustacean and a cephalopod. This research looks for evidence of differential microhabitat use of Aggregata patagonica infrapopulations between two zones of the digestive tract of his definitive host

  16. The role of tobacco as an etiological agent for oral cancer: Cytomorphometrical analysis of the buccal mucosa in tobacco users

    Directory of Open Access Journals (Sweden)

    Megha Singh

    2014-01-01

    Conclusion: The results confirmed that tobacco chewing and smoking influenced the cytomorphology of normal appearing buccal mucosa and the degree of these changes were found to be greater in chewers as compared to smokers.

  17. Neutrophil gelatinase-associated lipocalin (NGAL/Lcn2) is upregulated in gastric mucosa infected with Helicobacter pylori

    DEFF Research Database (Denmark)

    Alpízar-Alpízar, Warner; Laerum, Ole Didrik; Illemann, Martin

    2009-01-01

    characterized here the pattern of expression of NGAL/Lcn2 in gastric mucosa (45 non-neoplastic and 38 neoplastic tissue samples) and explored the connection between NGAL/Lcn2 expression and H. pylori infection. Immunohistochemical analysis showed high NGAL/Lcn2 expression in normal and gastritis-affected mucosa...... compared to low expression in intestinal metaplasia, dysplasia, and gastric cancer. In normal and gastritis-affected mucosa (n=36 tissue samples), NGAL/Lcn2 was more frequently seen in epithelial cells located at the neck and base of the glands in H. pylori-positive cases than in similar epithelial cells...... of noninfected cases (Fisher's exact test, p=0.04). In conclusion, the high expression of NGAL/Lcn2 in normal and gastritis-affected mucosa infected with H. pylori suggests that NGAL/Lcn2 is upregulated locally in response to this bacterial infection. It is discussed whether this may have a causal relation...

  18. Primary mucosa-associated lymphoid tissue (MALT) lymphoma of thyroid gland arising from coexisting Hashimoto's thyroiditis: a case report

    International Nuclear Information System (INIS)

    Lee, Sang Kwon; Kwon, Sun Young; Kim, Young Hwan; Choi, Jin Soo; Sohn, Chul Ho; Lee, Hee Jung; Woo, Seong Ku; Suh, Soo Ji

    2006-01-01

    We report herein on a case of primary mucosa-associated lymphoid tissue (MALT) lymphoma of the thyroid gland in a 57-year-old woman with coexisting Hashimoto's thyroiditis, and we include its characteristic imaging, histopathologic and immunohistochemical findings

  19. Uso del polen de abeja en la alimentación de pollos de engorda

    OpenAIRE

    de Oliveira, Maria Cristina; Loch, Fernando Carlos; Montes da Silva, Diones; Carneiro Martins, Poliana; Teixeira, Adriely Suzian; Cunha Claro, Daniel

    2015-01-01

    El polen de abeja (PA) puede ser un complemento nutricional para los animales, ya que mejora la eficiencia del uso de nutrientes, lo que aumenta su absorción, acelera el crecimiento de los animales y mejora su rendimiento productivo. El efecto del PA en la dieta de pollos de engorda se evaluó sobre la digestibilidad, rendimiento, mucosa intestinal y calidad de la cama. Para evaluar la digestibilidad, se utilizaron 200 aves en un diseño de bloques al azar con cuatro tratamientos (0, 0.5, 1.0 y...

  20. Wound healing in the jugal mucosa of rats with a cold blade scalpel and an ultrasonic harmonic scalpel

    OpenAIRE

    Masi,Elen Carolina David João De; Rocha,Sergio Luis; Mocellin,Marcos; Faria,João Luis Garcia de

    2009-01-01

    Ultrasound harmonic scalpel has been recently introduced in otorhinolaryngological procedures. AIM: to assess macro and microscopic evolution of the healing process of wounds created in the jugal mucosa of rats by the use of ultrasound scalpel. METHOD: we used 30 Wistar rats in which we made mucosal incisions on the right jugal mucosa with the ultrasound harmonic scalpel (USHS) and on the left side with the cold blade scalpel (CBS). Macroscopic and microscopic evaluations were carried out on ...

  1. Safe and successful birth following pelvic radiotherapy for rectal mucosa-associated lymphoid tissue lymphoma: a case report.

    Science.gov (United States)

    Hatayama, Yoshiomi; Aoki, Masahiko; Kawaguchi, Hideo; Hirose, Katsumi; Sato, Mariko; Akimoto, Hiroyoshi; Tanaka, Mitsuki; Fujioka, Ichitaro; Ono, Shuichi; Takai, Yoshihiro

    2017-02-01

    Mucosa-associated lymphoid tissue lymphomas can occur in various parts of the body, and half of mucosa-associated lymphoid tissue lymphomas occur in the gastrointestinal tract. Gastric mucosa-associated lymphoid tissue lymphoma is the most common lymphoma of the gastrointestinal tract and primary rectal mucosa-associated lymphoid tissue lymphoma is very rare. Because of the high radiosensitivity of mucosa-associated lymphoid tissue lymphomas, this condition can be controlled with radiotherapy of approximately 30 Gy alone. However, ovarian dysfunction as an adverse event of radiotherapy for pelvic lesions can become a problem in girls and women. We report a case of a 28-year-old woman with rectal mucosa-associated lymphoid tissue lymphoma who safely gave birth to a baby following 30.6 Gy radiotherapy to her whole rectum. A 28-year-old Japanese woman became aware of bloody stools and was diagnosed as having Lugano I rectal mucosa-associated lymphoid tissue lymphoma. She was referred to our institute and initiated on radiotherapy. However, she expressed a desire to bear children. We used horizontally opposed pair fields for radiotherapy to minimize the irradiation to her endometrium and ovary. A total dose of 30.6 Gy was given in 17 fractions of 1.8 Gy by 10-Megavolt X-ray linear accelerator. As a result, one-third of her uterus and half of her ovary were outside the irradiation field. After approximately 1 year of treatment, positive pregnancy was confirmed and finally she safely gave birth to a baby girl without congenital abnormalities. This report provides hope for girls and women who have undergone irradiation for pelvic mucosa-associated lymphoid tissue lymphomas and who desire to bear children.

  2. Manejo del esófago de Barrett: del tamizaje a los nuevos tratamientos

    Directory of Open Access Journals (Sweden)

    V. Thoguluva Chandrasekar

    2016-04-01

    Full Text Available El esófago de Barrett es un trastorno premaligno del esófago en el cual el epitelio escamoso de la porción distal del esófago es reemplazado por epitelio columnar. Debido a que la incidencia de adenocarcinoma esofágico se encuentra al alza, la mayoría de las sociedades de Gastroenterología han emitido sus propias recomendaciones para el tamizaje y la vigilancia. Factores específicos como la obesidad, la raza blanca, la edad por encima de los 50 años, el inicio del ERGE a edad temprana, el tabaquismo y la hernia hiatal han sido identificados como factores que incrementan el riesgo de esófago de Barrett y adenocarcinoma. El diagnóstico requiere tanto de la identificación endoscópica de mucosa con revestimiento columnar como de la confirmación histológica con biopsia. La mayoría de las sociedades médicas recomiendan tamizar a todas las personas con ERGE, así como aquellos con otros factores de riesgo con endoscopia; sin embargo, otras alternativas que utilizan métodos menos invasivos se encuentran bajo estudio en la actualidad. Las estrategias de vigilancia varían dependiendo de los hallazgos endoscópicos y se recomienda el protocolo de biopsias de Seattle con un muestreo de 4 cuadrantes aleatorizado. Algunos biomarcadores han mostrado resultados prometedores, aunque se requieren de más estudios en el futuro. La endoscopia de luz blanca es el estándar en la práctica, sin embargo, otras modalidades de imagen más avanzadas han mostrado resultados variables y, por lo tanto, se esperan más estudios para obtener validación adicional. Las técnicas de erradicación endoscópica, incluyendo tanto la resección como la ablación, han mostrado buenos resultados, aunque variables, en el tratamiento de lesiones displásicas confinadas a la mucosa. Los procedimientos de resección para remover las lesiones visibles seguida por la ablación de la mucosa displásica han mostrado los mejores resultados, con tasas de erradicación m

  3. Studies of blood flow in human nasal mucosa with /sup133/Xe washout technique and laser doppler flowmetry

    International Nuclear Information System (INIS)

    Olsson, P.

    1986-01-01

    The techniques were applied for studies of the influence of environmental temperature on the human nasal mucosa, for studies of mediators in nasal allergy and for studies of the sympathetic neurogenic control of blood flow in the nasal mucosa. The results show that the two techniques are complementary to one another. The /sup133/Xe washout technique is useful for semiquantitative estimations of blood flow in the deeper parts of the mucosa, while the laser doppler technique is especially suited for continuous recordings of relative blood flow changes in the superficial part of the mucosa. Vascular changes may take part in body temperature regulation changes may take part in body temperature regulation as well as in conditioning of respiratory air. The results support the theories that changes in nasal mucosal blood flow are related to body temperature control, while conditioning of inspiratory air may be more dependent on mucosal blood content. The observed dissociation between changes in the resistance and the capacitance vessels also illustrates that these vascular segments are regulated in different ways. The present results indicate that leukotriene D/sub4/ might contribute to an increased blood flow in the nasal mucosa and to blockage of the nasal airway in the acute allergic reaction. Vasomotion is demonstrated to be present in the nasal mucosa, and it appears to be partly dependent on sympathetic neurogenic activity. The development of the present techniques, means that vascular changes involved in normal nasal function and in nasal disease may be evaluated by a new approach. (author)

  4. Food restriction beginning at lactation interferes with the cellular dynamics of the mucosa and colonic myenteric innervation in adult rats

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    JOÃO PAULO F. SCHOFFEN

    2014-12-01

    Full Text Available The effects of food restriction (FR on the morphoquantitative aspects of the wall and myenteric neurons of the proximal colon in adult rats were analysed. FR was imposed by duplication of the experimental brood size in relation to the control brood during lactation. The FR group received a 50% reduction of food from weaning until 90 days of age. Samples of the colon underwent histological processing to morphometrically analyze the crypts, muscularis mucosae, tunica mucosa, and muscularis externa. We determined the number of goblet cells and serotoninergic enteroendocrine cells, and morphoquantitatively studied the myenteric neuronal population. FR caused hypertrophy in the tunica mucosa, increase in crypt depth and in the muscular layer of the mucosa, a decrease in the thickness of the tunica muscularis and in the number of goblet cells and an increase in serotoninergic cells. A higher neuronal density in the ganglia and a reduction of the cell profile area were observed in the FR group. FR imposed since lactation led to hypertrophy of the tunica mucosa, a reduction of neutral mucin production, atrophy of the tunica muscularis, and an increase in the survival neuronal in adult rats, attributable to an increase in the number of serotoninergic enteroendocrine cells in mucosa.

  5. Epithelial cell proliferation in human fundic and antral mucosae. Influence of superselective vagotomy and relationship with gastritis

    International Nuclear Information System (INIS)

    Gutierrez, O.; Lehy, T.; Rene, E.; Gres, L.; Bonfils, S.

    1985-01-01

    Epithelial cell proliferation in the fundic and antral mucosae was studied in 19 duodenal ulcer patients, 11 patients having undergone fundic superselective vagotomy for duodenal ulcer, and 10 controls. This was achieved through in vitro incorporation of tritiated thymidine in mucosal biopsies and radioautography. Except for increased fundic mucosal height, duodenal ulcer patients did not differ from controls for all parameters studied. In vagotomized patients, as compared to the other two groups, the labeling index was significantly enhanced in the innervated antral mucosa where atrophic gastritis developed, but there was no change in the labeling index and no worsening of mucosal inflammation in the denervated fundic mucosa. The only abnormality in the latter was a striking expansion, towards the surface, of the proliferative area within the fundic pit. The labeling indices and the degree of gastritis in gastric mucosae are significantly correlated in control and duodenal ulcer patients. If findings in antral mucosa, after superselective vagotomy, seemed related to gastritis lesions, those in fundic mucosa were not and may indicate an alteration due to the vagotomy per se

  6. A study to analyze the different patterns of quid usage among subjects with chewer′s mucosa

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    Saba Khan

    2012-01-01

    Full Text Available Aim: Betel chewer′s mucosa, although not considered as a potentially malignant lesion, has been suggested to be a precursor of oral submucous fibrosis (OSMF. Thus a study was undertaken to asses various quid chewing patterns in patients with chewer′s mucosa and to evaluate possible association between chewer′s mucosa and a particular quid usage patter. Materials and methods: The study was done on 150 subjects clinically diagnosed of having chewers mucosa lesion. After complete oral examination, a detailed habit history was taken through preformed questionnaire. The data obtained was analyzed using Chi-square test. Results: Among the 150 subjects males to female ratio was 8:2- Majority of the subjects were within the age group of 20 to 30 years and chewed a combination of betel leaf, arecanut, tobacco, lime- Majority of the subjects of chewer′s mucosa used the quid for a duration of 1 to 5 years, a frequency of three to five quid per day. Conclusion: In the present study, chewers mucosa was seen in majority of the subjects who used the quid for a duration of 1 to 5 years, a frequency of three to five quid per day and chewed the quid containing betel leaf, arecanut, tobacco, lime as its constituents.

  7. Theoretical Considerations and a Mathematical Model for the Analysis of the Biomechanical Response of Human Keratinized Oral Mucosa

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    Aikaterini Tsaira

    2016-08-01

    Full Text Available Removable complete and partial dentures are supported by the residual alveolar ridges consisting of mucosa, submucosa, periosteum and bone. An understanding of the biomechanical behavior of the oral mucosa is essential in order to improve the denture-bearing foundations for complete and partially edentulous patients. The purpose of this paper was to examine the biomechanical behavior of the soft tissues supporting a removable denture and develop a model for that reason. Keratinized oral mucosa blocks with their underlying bone were harvested from the maxillary palatal area adjacent to the edentulous ridges of a cadaver. The compressive response of the oral mucosa was tested by using atomic force microscopy. The specimens were first scanned in order their topography to be obtained. The mechanical properties of the specimens were tested using a single crystal silicon pyramidal tip, which traversed towards the keratinized oral mucosa specimens. Loading-unloading cycles were registered and four mathematical models were tested using MATLAB to note which one approximates the force-displacement curve as close as possible: a. spherical, b. conical, c. third order polynomial, d. Murphy (fourth order polynomial, non-linear Hertzian based. The third order polynomial model showed the best accuracy in representing the force-displacement data of the tested specimens. A model was developed in order to analyze the biomechanical behavior of the human oral keratinized mucosa and obtain information about its mechanical properties.

  8. Oral Mucosa Harbors a High Frequency of Endothelial Cells: A Novel Postnatal Cell Source for Angiogenic Regeneration.

    Science.gov (United States)

    Zhou, Jian; Rogers, Jason H; Lee, Scott H; Sun, DongMing; Yao, Hai; Mao, Jeremy J; Kong, Kimi Y

    2017-01-15

    Endothelial progenitor cells/endothelial cells (EPCs/ECs) have great potential to treat pathological conditions such as cardiac infarction, muscle ischemia, and bone fractures, but isolation of EPC/ECs from existing cell sources is challenging due to their low EC frequency. We have isolated endothelial progenitor (EP)-like cells from rat oral mucosa and characterized their yield, immunophenotype, growth, and in vivo angiogenic potential. The frequency of EP-like cells derived from oral mucosa is thousands of folds higher than EPCs derived from donor-match bone marrow samples. EP-like cells from oral mucosa were positive for EC markers CD31, VE-Cadherin, and VEGFR2. Oral mucosa-derived EP-like cells displayed robust uptake of acetylated low-density lipoprotein and formed stable capillary networks in Matrigel. Subcutaneously implanted oral mucosa-derived EP-like cells anastomosed with host blood vessels, implicating their ability to elicit angiogenesis. Similar to endothelial colony-forming cells, EP-like cells from oral mucosa have a significantly higher proliferative rate than human umbilical vein endothelial cells. These findings identify a putative EPC source that is easily accessible in the oral cavity, potentially from discarded tissue specimens, and yet with robust yield and potency for angiogenesis in tissue and organ regeneration.

  9. Il dono del racconto

    DEFF Research Database (Denmark)

    Polito, Paola

    1998-01-01

    L'articolo indaga a vari livelli strutturali del romanzo "Il coraggio del pettirosso" (rete metaforica, dialogismo diegetico, dialogismo come fenomeno di voce, strategie discorsive messe in atto dalla voce narrante, stile colloquiale) la complessa realizzazione testuale del genere narrativo...

  10. Tatuagem extensa por amálgama em mucosa gêngivo-alveolar Extensive amalgam tattoo on the alveolar-gingival mucosa

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    Vivian C Galletta

    2011-10-01

    Full Text Available Tatuagens por amálgama são lesões pigmentadas, exógenas, de frequente ocorrência na mucosa bucal, que resultam da introdução acidental de partículas de amálgama nos tecidos moles. O diagnóstico da tatuagem por amálgama é simples, geralmente, baseado em achados clínicos, complementado pela história recente ou pregressa de remoção de restauração por amálgama. Radiografias intraorais podem ser úteis na detecção de radiopacidade, associadas à partícula de amálgama. Nos casos em que as tatuagens por amálgama não permitem diferenciação de outras lesões melanocíticas, o exame histopatológico deve ser realizado. Os autores relatam à ocorrência de lesão extensa por tatuagem de amálgama com confirmação histopatológica.Amalgam tattoos are common exogenous pigmented lesions of the oral mucosa occurring mainly by inadvertent placement of amalgam particles into soft tissues. The diagnosis of amalgam tattoo is simple, usually based on clinical findings associated with presence or history of amalgam fillings removal. Intraoral X-rays may be helpful in detecting amalgam-related radiopacity. In cases where amalgam tattoo cannot be differentiated from other causes of oral pigmentation, a biopsy should be performed. This article deals with an extensive amalgam tattoo lesion which required a biopsy for a definitive diagnosis.

  11. A mucosa oral na hanseníase: um estudo clínico e histopatológico The oral mucosa in leprosy: a clinical and histopathological study

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    Marilda Aparecida Milanez Morgado de Abreu

    2006-06-01

    Full Text Available INTRODUÇÃO: a anseníase multibacilar pode causar comprometimento da mucosa oral, com ou sem lesões aparentes. Há poucos estudos que tratam deste assunto na era da multidrogaterapia. OBJETIVO: Verificar a freqüência do comprometimento da mucosa oral em pacientes de hanseníase multibacilar. CASUÍSTICA E MÉTODOS: Foi realizado um estudo transversal em vinte pacientes de hanseníase multibacilar, não-tratados, atendidos consecutivamente em Dracena, São Paulo, entre o período de 2000 e 2002. Foi realizado exame clínico completo da mucosa oral. Os pacientes foram submetidos a biópsias na mucosa jugal, na língua e no palato mole, em alteração ou em pontos pré-estabelecidos. Os cortes foram corados pelas técnicas da hematoxilina-eosina e Ziehl-Neelsen. O encontro de granuloma e bacilos álcool-ácido-resistentes ao exame histopatológico determinou o comprometimento específico. RESULTADOS: O estudo envolveu 19 pacientes multibacilares com tempo médio de evolução de 2,5 anos. Ocorreu comprometimento histopatológico específico em apenas um paciente virchowiano, com mucosa oral clinicamente normal, na língua e no palato mole. CONCLUSÕES: 1. Alteração clínica na mucosa oral não implica em comprometimento pela doença, é necessário confirmação histopatológica. 2. Alterações clínicas específicas aparentes são raras. 3. A mucosa oral clinicamente normal pode exibir comprometimento histopatológico específico.INTRODUCTION: Multibacillary leprosy may involve the oral mucosa, with or without apparent lesions. There are few studies that deal with this issue in the era of multidrug therapy. AIM: To assess the frequence of oral mucosa involvement in multibacillary leprosy patients. PATIENTS AND METHODS: A transversal study with twenty non-treated multibacillary leprosy patients. The patients were treated in Dracena, São Paulo, between 2000 and 2002. Clinical examination of the oral mucosa was carried out. All patients were

  12. del cambio

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    Alejandro Canales

    2006-01-01

    Full Text Available El artículo analiza las propuestas y los compromisos en materia de cobertura y de financiamiento en el periodo 2000-2006. En particular, revisa las modificaciones y los ajustes de los tres principales documentos en los que se expresaron las iniciativas: el de la Asociación Nacional de Universidades e Instituciones de Educación Superior, el del equipo de la transición y el programa sectorial. Los resultados muestran que las cifras de atención a la demanda educativa e inversión quedaron lejos de las metas autoimpuestas por el gobierno federal y que será necesario realizar mayores esfuerzos para atender tales desafíos.

  13. Immunohistochemical study of jejunal graft mucosa cell populations during the initial adaptation phase in the host body in rats.

    Science.gov (United States)

    Tóth, Stefan; Jonecová, Zuzana; Varga, Ján; Staško, Pavel; Kovalčinová, Barbora; Maretta, Milan; Leško, Dušan; Veselá, Jarmila

    2013-10-01

    The character of the changes in cell populations within the jejunal graft mucosa during the initial adaptation phase in the host body was investigated. 24 adult male Wistar rats underwent intestinal heterotopic allotransplantation. Aorto-aortal and porto-caval anastomoses were performed using the end-to-side microsurgery technique. Graft tissues were compared to the intestinal tissues of the recipients. This study demonstrates that: (1) Distinct injury to the graft mucosa 1h after transplantation was accompanied by significant reduction in numbers of epithelial secretory cell populations. The injury was more intense in the mesenteric portion. Six hours after transplantation the graft mucosa was covered by a continuous epithelium, but the number of goblet and Paneth cells was found to be less than 30% of that in the recipient epithelium. (2) In comparison with recipients, myeloperoxidase-positive cell numbers increased significantly in the graft mucosa 1 h after transplantation. In the epithelial layer, denudation and destruction of villi was associated with a significant reduction in intraepithelial lymphocyte numbers. A significant decrease in mucosal mast cell numbers was detected 6 h after transplantation. They attained only 10% of the number found in the recipients. (3) Time-dependent changes in the graft mucosa revealed that CD163-positive cells increased significantly in the graft mucosa during 6 h after transplantation and reached the level found in the recipients. In contrast, the myeloperoxidase-positive cell population significantly decreased in the graft mucosa within the initial 6 h. Copyright © 2013 Elsevier GmbH. All rights reserved.

  14. A comparative study of clinicopathological features between chronic cholecystitis patients with and without Helicobacter pylori infection in gallbladder mucosa.

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    Di Zhou

    Full Text Available BACKGROUND: Helicobacter pylori has been isolated from 10%-20% of human chronic cholecystitis specimens but the characteristics of "Helicobacter pylori positive cholecystitis" remains unclear. This study aims to compare the clinicopathological features between chronic cholecystitis patients with and without Helicobacter pylori infection in gallbladder mucosa. METHODS: Three hundred and twenty-six chronic cholecystitis patients were divided into two groups according to whether Helicobacter pylori could be detected by culture, staining or PCR for Helicobacter 16s rRNA gene in gallbladder mucosa. Positive samples were sequenced for Helicobacter pylori-specific identification. Clinical parameters as well as pathological characteristics including some premalignant lesions and the expression levels of iNOS and ROS in gallbladder were compared between the two groups. RESULTS: Helicobacter pylori infection in gallbladder mucosa was detected in 20.55% of cholecystitis patients. These patients had a higher prevalence of acid regurgitation symptoms (p = 0.001, more histories of chronic gastritis (p = 0.005, gastric ulcer (p = 0.042, duodenal ulcer (p = 0.026 and higher presence of Helicobacter pylori in the stomach as compared to patients without Helicobacter pylori infection in the gallbladder mucosa. Helicobacter pylori 16s rRNA in gallbladder and gastric-duodenal mucosa from the same individual patient had identical sequences. Also, higher incidences of adenomyomatosis (p = 0.012, metaplasia (p = 0.022 and higher enhanced expressions of iNOS and ROS were detected in Helicobacter pylori infected gallbladder mucosa (p<0.05. CONCLUSIONS: Helicobacter pylori infection in gallbladder mucosa is strongly associated with Helicobacter pylori existed in stomach. Helicobacter pylori is also correlated with gallbladder premalignant lesions including metaplasia and adenomyomatosis. The potential mechanism might be related with higher ROS

  15. EGFR and Bcl-2 in gastric mucosa of children infected with Helicobacter pylori

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    Ewa Ryszczuk

    2016-03-01

    Full Text Available Aim: The aim of the study was to evaluate the expression of EGFR and Bcl-2 proteins as inhibitory markers of apoptosis in surface epithelial cells and gland cells of antral gastric mucosa in children infected with Helicobacter pylori according to the severity and activity of antral gastritis and to assess the correlation between the number of cells expressing EGFR and the number of cells expressing Bcl-2 in H. pylori infected children.Materials and methods: The study included 44 children: 68.2% with chronic gastritis and positive IgG against H. pylori, and 31.8% with functional disorders of the gastrointestinal tract and with normal IgG against H. pylori. The evaluation of EGFR expression in gastric mucosa was performed immunohistochemically using monoclonal mouse anti-EGFR antibody. The polyclonal antibody was used to determine the expression of anti-Bcl-2.Results: A significant increase in the number of cells expressing EGFR and Bcl-2 protein was found in the epithelial cells in severe as well as mild and moderate gastritis in the group of children infected with H. pylori. An increase in the number of cells expressing EGFR and Bcl-2 protein was also found in the epithelial cells in group I according to the activity of gastritis. There was a statistically significant positive correlation between the numbers of cells expressing EGFR and Bcl-2 in H. pylori infected children.Conclusion: Increased expression of EGFR and Bcl-2 proteins in the epithelial cells and a statistically significant positive correlation between the numbers of cells expressing EGFR and Bcl-2 in H. pylori infected children could suggest increased regeneration abilities of gastric mucosa.

  16. Candida species isolated from the vaginal mucosa of HIV-infected women in Salvador, Bahia, Brazil

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    Paula Matos Oliveira

    Full Text Available BACKGROUND: Vulvovaginal candidiasis (VVC is the second most common vaginal infection. HIV-infection is a risk factor for this infection. OBJECTIVE: To determine the frequency of VVC and to describe the main Candida species isolated and their susceptibility to antifungal drugs in HIV-infected patients, compared to HIV-uninfected women in Salvador, Brazil. METHODS: Cross-sectional study including a group of 64 HIV-infected women and 76 uninfected women, followed up at the AIDS reference center and at the Gynecological Clinic of Escola Bahiana de Medicina e Saúde Pública (Salvador, Bahia, Brazil. RESULTS: Frequency of Candida spp. was higher in HIV-infected women (29.7% than in HIV-uninfected controls (14.5% (p = 0.02. The odds ratio value for vulvovaginal candidiasis in HIV-infected patients was 2.6 (95% CI: 1.07 - 6.32 p = 0.03. Candida albicans was the most commonly isolated species in both HIV-infected (52.3% and uninfected women (85.7%, followed by C. parapsolis in 17.6% and 14.3%, respectively. In HIV-infected women, C. glabrata, C. parapsilosis, and a coinfection of C. albicans and C. glabrata were also identified. There was no significant difference between Candida species isolated from the vaginal mucosa of women with VVC and colonization of the vaginal mucosa of HIV-infected and HIV-uninfected women. One C. glabrata isolate from an HIV-infected patient was resistant to fluconazole and other two isolates exhibited a dose-dependent susceptibility. CONCLUSION: Our results confirm a higher frequency of Candida spp. isolated from the vaginal mucosa of HIV-infected women and a broader spectrum of species involved. Only Candida glabrata isolates showed decreased susceptibility to fluconazole.

  17. The inflammatory and normal transcriptome of mouse bladder detrusor and mucosa

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    Dyer David W

    2006-01-01

    Full Text Available Abstract Background An organ such as the bladder consists of complex, interacting set of tissues and cells. Inflammation has been implicated in every major disease of the bladder, including cancer, interstitial cystitis, and infection. However, scanty is the information about individual detrusor and urothelium transcriptomes in response to inflammation. Here, we used suppression subtractive hybridizations (SSH to determine bladder tissue- and disease-specific genes and transcriptional regulatory elements (TREs. Unique TREs and genes were assembled into putative networks. Results It was found that the control bladder mucosa presented regulatory elements driving genes such as myosin light chain phosphatase and calponin 1 that influence the smooth muscle phenotype. In the control detrusor network the Pax-3 TRE was significantly over-represented. During development, the Pax-3 transcription factor (TF maintains progenitor cells in an undifferentiated state whereas, during inflammation, Pax-3 was suppressed and genes involved in neuronal development (synapsin I were up-regulated. Therefore, during inflammation, an increased maturation of neural progenitor cells in the muscle may underlie detrusor instability. NF-κB was specifically over-represented in the inflamed mucosa regulatory network. When the inflamed detrusor was compared to control, two major pathways were found, one encoding synapsin I, a neuron-specific phosphoprotein, and the other an important apoptotic protein, siva. In response to LPS-induced inflammation, the liver X receptor was over-represented in both mucosa and detrusor regulatory networks confirming a role for this nuclear receptor in LPS-induced gene expression. Conclusion A new approach for understanding bladder muscle-urothelium interaction was developed by assembling SSH, real time PCR, and TRE analysis results into regulatory networks. Interestingly, some of the TREs and their downstream transcripts originally involved in

  18. [The effect of hypertonic seawater and isotonic seawater for nasal mucosa of allergic rhinitis mice model].

    Science.gov (United States)

    Deng, Zhifeng; Xu, Yu; Ou, Jin; Xiang, Rong; Tao, Zezhang

    2014-12-01

    To study the effect of hypertonic seawater and isotonic seawater for nasal mucosa of allergic rhinitis mice model, and explore the possible mechanism of nasal irrigation with seawater in treatment of allergic rhinitis. We used Der pl to make allergic rhinitis model of BALB/c mice, and divided them into three groups randomly. Nasal irrigation with hypertonic seawater (HS) or isotonic seawater (IS) in the treatment group 1-14 days after modeling, and black control (BC) group was given no treatment after modeling. Normal control (NC) group was given no treatment, the number of rubs and sneezings in each group were counted in 30 min after the last nasal irrigation. Mice were then killed 24 h after the last therapy. The noses of mice from each group were removed and fixed, then the slices were stained with hematoxylin and eosin, the others were observed by transmission electron microscope. Mice with hypertonic seawater and isotonic seawater were significantly improved in rubs and sneezings compared to the black control group (P 0. 05); Ciliated columnar epithelium cells in mucosal tissues of HS group and IS group were arranged trimly, better than that in the black control group. Morphology and microstructure in nasal mucosal of HS group was closer to the normal group than in IS group. The injury of nasal mucosa ciliated epithelium was significantly improved by nasal irrigation with hypertonic seawater and isotonic seawater, and the former is better than the latter, the mechanism of nasal irrigation with seawater in treatment of allergic rhinitis may rely on repairing the injured nasal mucosa ciliated epithelium, thereby the symptoms of nasal was reduced.

  19. Ectopic Compound Odontoma in the Buccal Mucosa: Report of a Rare Case

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    Aparna Venigalla

    2015-01-01

    Full Text Available Eruption of tooth into extraosseous locations is an extremely rare condition. We report a case of a six-year-old girl child with tooth-like structure erupting from the right buccal mucosa. Clinical, radiographic, and histopathologic examination suggested the diagnosis of compound odontoma. Very few cases have been reported so far, where tooth has been located completely in the soft tissue and a variety of names have been used for that condition. A brief review of the literature and the ambiguity in naming the situation is discussed.

  20. Outcome of buccal mucosa urethroplasty in the management of urethral strictures

    OpenAIRE

    Basri Cakiroglu; Orhun Sinanoglu; Ersan Arda

    2017-01-01

    Objective: The objective of the study is to report the outcome of buccal mucosal urethroplasty. Materials and methods: The follow up data of 15 patients undergoing single stage urethroplasty from September 2010 to September 2015 were retropectively reviewed. They received buccal mucosa graft for urethroplasty. The patients were followed for complications and outcome. Results: Mean age was 53.7 ± 13.6 The stricture length ranged from 3 to 6 cm (mean 4.4 ± 0.8). The success rate for buccal muco...

  1. TP53 mutations in clinically normal mucosa adjacent to oral carcinomas

    DEFF Research Database (Denmark)

    Thode, Christenze; Bilde, Anders; von Buchwald, Christian

    2010-01-01

    BACKGROUND: The tumour-suppressor protein p53 often accumulates in histologically normal epithelium adjacent to oral squamous cell carcinomas (OSCC). We investigated whether this was associated with mutations in TP53, the gene for p53, and might implicate impending malignancy. METHODS: Specimens...... products were separated by denatured gradient gel electrophoresis. Fragments with a deviant DGEE pattern were sequenced. RESULTS: TP53 mutations were found in six of 18 tumours. Fourteen specimens contained histologically normal mucosa adjacent to the tumour; 13 of these showed small clusters of p53...

  2. Localization and function of histamine H3 receptor in the nasal mucosa

    OpenAIRE

    Suzuki, Shinya; Takeuchi, Kazuhiko; Majima, Yuichi

    2008-01-01

    BACKGROUND: Histamine is an important chemical mediator of allergic rhinitis (AR). Histamine H3 receptors H3R are located on cholinergic and NANC neurons of the myenteric plexus, and activation of H3R regulates gastric acid secretion. However, little is known about the localization and function of H3R in the upper airway. OBJECTIVE: The objective of this study was to examine the localization and possible function of H3R in the nasal mucosa. METHODS: We extracted total RNA from the inferior tu...

  3. Studies on Cinical , Haematological Aspects and Pathological Changes of Gastric Mucosa in Geophagia

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    S.N. SAYAR

    1975-01-01

    Full Text Available Geophagia characterized by, severe, anaemia, dwarfism, hypogonadism • and hepatosplenomegaly is sometimes seen in young patients (and children in Iran. 2 Haematological aspects of the syndrome are those of, severe, iron defi-crency anaemia3 Gastric biopsies and histological findings revealed superfi cial or atrophic gastritis showing some resemblance to those seen in pernicious anaemia. 4 Haematological features, anaemia ana many of the clinical signs of the syndrome were improved after appropriate iron therapy. 5 Histological changes of gastric mucosa improved, in 5 patients, 6 months after correction of the anaemia.

  4. Effects of intranasal corticosteroids on radiated nasal mucosa of guinea pig

    International Nuclear Information System (INIS)

    Zhu Xinhua; Liu Yuehui

    2009-01-01

    Objective: To investigate a mechanism protected radiation-induce injure for radiated guinea pigs'nasal mucosa treated with intranasal corticosteroids(fluticasone nasal cavity spray). Methods: 50 health guinea pigs were divided into 2 groups randomly: the irradiated group (control group) with 25 guinea pigs and the administration group after irradiation (test group)with 25 guinea pigs. The nasal part of all guinea pigs were performed irradiation by the 6 MV X-ray with single 5 Gy, one time each week for three weeks. The guinea pigs of test group received intranasal corticosteroids with one time every day and one spray each side nasal cavity on the second day after three weeks irradiation. Five guinea pigs in each group were sacrificed randomly at 1 week, 1 month, 2 months, 3 months and 4 months after irradiation, and the histopathologic changes were observed under optical microscope and electron microscope. At the same time, blood were drawn from the heart and the concentration of IL-1, IL-6 and TNF-α in serum were measured by ELISA. Results: The early nasal mucosa inflammatory reaction of the test group was less than the control group. The coverage rate of cilia of the test group was much than that of the control group (72.9% vs 50.2%) at four months after irradiation. The atrophy of submucosal glandular organ was lessened and they displayed some extent secretory function. The concentration of IL-1 in serum was increased very much in the test group compared with the control group after irradiation and kept higher level in the first two months. After two months, it began to decrease; on four months, it still kept equivalency level with the control group. The concentration of IL-6 and TNF-α in serum were reduced all the while. Conclusions: The intranasal corticosteroids with fluticasone nasal cavity spray can reduce radiation- induced injury of guinea pigs' nasal mucosa. The concentration change of IL-1, IL-6 and TNF-α in serum may be one of mechanism protected

  5. Compartmentalised expression of meprin in small intestinal mucosa: enhanced expression in lamina propria in coeliac disease.

    Science.gov (United States)

    Lottaz, Daniel; Buri, Caroline; Monteleone, Giovanni; Rösmann, Sandra; Macdonald, Thomas T; Sanderson, Ian R; Sterchi, Erwin E

    2007-03-01

    Epithelial cells in the human small intestine express meprin, an astacin-like metalloprotease, which accumulates normally at the brush border membrane and in the gut lumen. Therefore, meprin is targeted towards luminal components. In coeliac disease patients, peptides from ingested cereals trigger mucosal inflammation in the small intestine, disrupting epithelial cell differentiation and function. Using in situ hybridisation on duodenal tissue sections, we observed a marked shift of meprin mRNA expression from epithelial cells, the predominant expression site in normal mucosa, to lamina propria leukocytes in coeliac disease. Meprin thereby gains access to the substrate repertoire present beneath the epithelium.

  6. Primary mucosa-associated lymphoid tissue lymphoma of the breast: a case report

    Energy Technology Data Exchange (ETDEWEB)

    Park, Jong Heon; Kim, Dae Bong; Shin, Mi Kyung; Jang, Suk Ki; Kang, Su Min; Ahn, In Oak [Bundang Jesaeng General Hospital, Seongnam (Korea, Republic of)

    2007-11-15

    A primary mucosa-associated lymphoid tissue lymphoma (MALT lymphoma) of the breast is extremely rare. We report a case of a MALT lymphoma of the breast that presented as a palpable left breast mass in a 37-year-old woman. A physical examination revealed a large firm, and fixed mass in the left inner breast. Mammograms showed a large, isodense mass in the lower inner quadrant of the left breast and an enlarged lymph node in the axilla. A sonogram demonstrated a 5 cm sized, oval, circumscribed, and heterogeneously hypoechoic mass with posterior acoustic enhancement. A surgical biopsy was performed, and the pathology revealed a MALT lymphoma.

  7. Primary mucosa-associated lymphoid tissue lymphoma of the breast: a case report

    International Nuclear Information System (INIS)

    Park, Jong Heon; Kim, Dae Bong; Shin, Mi Kyung; Jang, Suk Ki; Kang, Su Min; Ahn, In Oak

    2007-01-01

    A primary mucosa-associated lymphoid tissue lymphoma (MALT lymphoma) of the breast is extremely rare. We report a case of a MALT lymphoma of the breast that presented as a palpable left breast mass in a 37-year-old woman. A physical examination revealed a large firm, and fixed mass in the left inner breast. Mammograms showed a large, isodense mass in the lower inner quadrant of the left breast and an enlarged lymph node in the axilla. A sonogram demonstrated a 5 cm sized, oval, circumscribed, and heterogeneously hypoechoic mass with posterior acoustic enhancement. A surgical biopsy was performed, and the pathology revealed a MALT lymphoma

  8. Primary mucosa-associated lymphoid tissue thyroid lymphoma: a rare thyroid neoplasm of extrathyroid origin

    Directory of Open Access Journals (Sweden)

    Dimitrios Hadjidakis

    2012-01-01

    Full Text Available Primary thyroid lymphoma is a rare malignancy, representing 2-8% of all thyroid malignancies and 1-2% of all extranodal lymphomas. The majority of cases concern non-Hodgkin`s lymphoma of B cell origin, following by Hodgkin’s disease, T cell lymphomas and rarely marginal zone B-cell mucosa-associated lymphoid tissue (MALT lymphomas. MALT lymphomas have been associated with long-standing autoimmune Hashimoto`s thyroiditis. We present the case of a 44-years-old woman with thyroid MALT lymphoma in the background of multinodular goiter of autoimmune origin.

  9. Regulation of serotonin release from enterochromaffin cells of rat cecum mucosa

    International Nuclear Information System (INIS)

    Simon, C.; Ternaux, J.P.

    1990-01-01

    The release of endogenous serotonin or previously taken up tritiated serotonin from isolated strips of rat cecum mucosa containing enterochromaffin cells was studied in vitro. Release of tritiated serotonin was increased by potassium depolarization and was decreased by tetrodotoxin, veratridine and the absence of calcium. Endogenous serotonin was released at a lower rate than tritiated serotonin; endogenous serotonin release was stimulated by potassium depolarization but was unaffected by tetrodotoxin, veratridine or the absence of calcium. Carbachol, norepinephrine, clonidine and isoproterenol decreased release of tritiated serotonin but had less or reverse effect on release of endogenous serotonin. The results suggest two different serotoninergic pools within the enterochromaffin cell population

  10. Carcinoma Buccal Mucosa Underlying a Giant Cutaneous Horn: A Case Report and Review of the Literature

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    Sunil Kumar

    2014-01-01

    Full Text Available Cutaneous horn is a conical, dense, and hyperkeratotic protrusion that often appears similar to the horn of an animal. Giant cutaneous horns are rare; no incidence or prevalence has been reported. The significance of cutaneous horns is that they occur in association with, or as a response to, a wide variety of underlying benign, premalignant, and malignant cutaneous diseases. A case of giant cutaneous horn of left oral commissure along with carcinoma left buccal mucosa is reported here as an extremely rare oral/perioral pathology.

  11. Impact of gastro-esophageal reflux on mucin mRNA expression in the esophageal mucosa.

    Science.gov (United States)

    van Roon, Aafke H C; Mayne, George C; Wijnhoven, Bas P L; Watson, David I; Leong, Mary P; Neijman, Gabriëlle E; Michael, Michael Z; McKay, Andrew R; Astill, David; Hussey, Damian J

    2008-08-01

    Changes in the expression of mucin genes in the esophageal mucosa associated with uncomplicated gastro-esophageal reflux disease have not been evaluated even though such changes could be associated with reflux-induced mucosal damage. We therefore sought to identify reflux-induced changes in mucin gene expression using a cell line and biopsies from the esophageal mucosa in patients with and without reflux. MUC-1, MUC-3, MUC-4, and MUC-5AC gene expressions were investigated in the HET-1A cell line following exposure to acid (pH 4) and/or bile (120 muM of a bile salt milieu), and in esophageal mucosal biopsies from controls, subjects with non-erosive gastro-esophageal reflux, and subjects with reflux associated with ulcerative esophagitis (erosive). The mucosal biopsies were also evaluated for IL-6 mRNA expression (inflammatory marker) and CK-14 mRNA expression (mucosal basal cell layer marker). Gene expression was determined using real-time reverse transcriptase-polymerase chain reaction analysis. In the cell line studies, there were differences in mRNA levels for all of the evaluated mucins following treatment with either acid or the acid and bile combination. In the studies which evaluated tissue specimens, IL-6 and CK-14 mRNA levels increased according to degree of reflux pathology. The expression of MUC-1 and MUC-4 in mucosa from patients with erosive reflux was lower than in subjects without reflux and in patients with non-erosive reflux, whereas the expression of MUC-3 and MUC-5AC was increased (although these differences did not reach significance at p reflux groups. The correlation between IL-6 and MUC-3 was significant within the control and erosive reflux groups, and the correlation between MUC-1 and MUC-5AC was significant within the erosive reflux group. The results of this study suggest that the profile of mucin expression in the esophageal mucosa is influenced by the pH and composition of the gastro-esophageal reflux. Further work should explore the

  12. Immunopathological modifications in the rectal mucosa from an animal model of food allergy Modificaciones inmunopatológicas de la mucosa rectal en un modelo animal de alergia alimentaria

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    M. Vinuesa

    2005-09-01

    Full Text Available Aim: the aim is to determine immunopathological modifications in rectal mucosa from rabbit after local challenge in sensitized animals with ovalbumin (OVA. Experimental design: thirty rabbits divided into three groups: G1: normal, G2: subcutaneously OVA sensitized, G3: sensitized, locally OVA challenged and sampled 4 hours after challenge. Specific anti-OVA IgE levels were evaluated by passive cutaneous anaphylaxis test (PCA. In each group 200 high microscopical power fields (HPF were counted. Results were expressed as arithmetic mean and SE. Statistical analysis was made using Student t test. Anti-CD4, CD5, µ chain, CD25 and RLA II monoclonal antibodies were used. Avidin biotin horseradish peroxidase system was used. Results: CD 4: G1: 8,3 ± 0,06; G2: 13,4 ± 0,08 and G3: 8,25 ± 0,06. CD 5: G1: 7,3 ± 0,05; G2: 9,4 ± 0,05 and G3: 11,3 ± 0,06. CD 25: G1: 13 ± 0,08; G2: 15,1 ± 0,13 and G3: 25,5 ± 0,15. m chain: G1: 10,4 ± 0,06; G2: 3,8 ± 0,02 and G3: 6,0 ± 0,10. RLA II (DR: G1: 11,6 ± 0,O5; G2: 19,2 ± 0,09 and G3: 19,1 ± 0,11. In all cases, experimental groups (G2 and G3 presented statistical significant differences vs. control group (G1 (p Objetivo: determinar las modificaciones inmunopatológicas en la mucosa rectal de conejo sensibilizado con ovoalbúmina (OVA y desafiado localmente. Diseño experimental: treinta conejos divididos en tres grupos G1: normal; G2 sensibilizado por vía subcutánea con OVA y G3: sensibilizado y desafiado localmente con OVA y muestreados 4 horas después del desafío. Los niveles de IgE anti-OVA específica fueron evaluados por el test de anafilaxia cutánea pasiva (PCA. Se contaron 200 campos de mayor aumento en cada grupo. Los resultados fueron expresados como media aritmética y error standard aplicándose el test de la t de Student. Resultados: CD 4: G1: 8,3 ± 0,06; G2: 13,4 ± 0,08 y G3: 8,25 ± 0,06. CD 5: G1: 7,3 ± 0,05; G2: 9,4 ± 0,05 y G3: 11,3 ± 0,06. CD 25: G1: 13., ± 0,08; G2: 15,1

  13. Development of a tissue-engineered human oral mucosa equivalent based on an acellular allogeneic dermal matrix: a preliminary report of clinical application to burn wounds.

    Science.gov (United States)

    Iida, Takuya; Takami, Yoshihiro; Yamaguchi, Ryo; Shimazaki, Shuji; Harii, Kiyonori

    2005-01-01

    Tissue-engineered skin equivalents composed of epidermal and dermal components have been widely investigated for coverage of full-thickness skin defects. We developed a tissue-engineered oral mucosa equivalent based on an acellular allogeneic dermal matrix and investigated its characteristics. We also tried and assessed its preliminary clinical application. Human oral mucosal keratinocytes were separated from a piece of oral mucosa and cultured in a chemically-defined medium. The keratinocytes were seeded on to the acellular allogeneic dermal matrix and cultured. Histologically, the mucosa equivalent had a well-stratified epithelial layer. Immunohistochemical study showed that it was similar to normal oral mucosa. We applied this equivalent in one case with an extensive burn wound. The equivalent was transplanted three weeks after the harvest of the patient's oral mucosa and about 30% of the graft finally survived. We conclude that this new oral mucosa equivalent could become a therapeutic option for the treatment of extensive burns.

  14. La superaci??n del modelo del ??ngel del hogar:

    OpenAIRE

    Hurtado Mu??oz, M??nica

    2012-01-01

    La Tesis Doctoral titulada "La superaci??n del modelo del "??ngel del hogar". Recuperaci??n de la escritora Leonor Canalejas y Fustegueras (1869-1945)" aborda, en primer lugar, un an??lisis sociol??gico de las circunstancias que rodearon a la mujer espa??ola en el cambio de siglo en el ??mbito social y educativo, en un intento de esclarecer las caracter??sticas propias del contexto en el que la mujer accede a la intelectualidad en general y a la literatura en particular. Esta parte concluye q...

  15. An experimental study of the radiation-induced changes in the epithelium of the rat palatal mucosae

    International Nuclear Information System (INIS)

    Ahn, Sang Hee; Lee, Kang Sook; Sohn, Jung Ick; Choi, Karp Shick

    1994-01-01

    The purpose of this study was to investigate the irradiation effects on the palatal mucosa. For this study, Sprague-Dawley strain rats were irradiated to their head and neck region with the dose of 5 Gy and 10 Gy by 6MV X-radiation and sacrificed on the experimental periods after irradiation. The authors observed the histological changes of the hard and soft palatal mucosae. The results were as follows; In the light microscopic examination, hydropic change on the basal cells, increased cell size of the epithelium, and decreased epithelial cell layers were observed on the 3 hours, 6 hours, and 12 hours groups after irradiation. But, basal cell hyperplasia, increased epithelial cell layers, and elongated rete pegs were observed on the 3 days group after irradiation. After then, these changes were recovered in the mucosa of the hard palate on the 7 days and 14 days groups, and in the mucosa of the soft palate on the 14 days and 28 days groups after irradiation. And such changes were greater in the mucosa of the soft palate than in that of the hard palate, and more prominent in 10 Gy irradiated groups than in 5 Gy irradiated groups.

  16. Molecular genotyping of anisakis larvae in Middle Eastern Japan and endoscopic evidence for preferential penetration of normal over atrophic mucosa.

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    Toshio Arai

    Full Text Available BACKGROUND: Anisakiasis is a parasitic disease caused primarily by Anisakis spp. larvae in Asia and in Western countries. The aim of this study was to investigate the genotype of Anisakis larvae endoscopically removed from Middle Eastern Japanese patients and to determine whether mucosal atrophy affects the risk of penetration in gastric anisakiasis. METHODS: In this study, 57 larvae collected from 44 patients with anisakiasis (42 gastric and 2 colonic anisakiasis were analyzed retrospectively. Genotyping was confirmed by restriction fragment length polymorphism (RFLP analysis of ITS regions and by sequencing the mitochondrial small subunit (SSU region. In the cases of gastric anisakiasis, correlation analyses were conducted between the frequency of larval penetration in normal/atrophic area and the manifestation of clinical symptoms. RESULTS: Nearly all larvae were A. simplex seusu stricto (s.s. (99%, and one larva displayed a hybrid genotype. The A. simplex larvae penetrated normal mucosa more frequently than atrophic area (p = 0.005. Finally, patients with normal mucosa infection were more likely to exhibit clinical symptoms than those with atrophic mucosa infection (odds ratio, 6.96; 95% confidence interval, 1.52-31.8. CONCLUSIONS: In Japan, A. simplex s.s. is the main etiological agent of human anisakiasis and tends to penetrate normal gastric mucosa. Careful endoscopic examination of normal gastric mucosa, particularly in the greater curvature of the stomach will improve the detection of Anisakis larvae.

  17. Molecular Genotyping of Anisakis Larvae in Middle Eastern Japan and Endoscopic Evidence for Preferential Penetration of Normal over Atrophic Mucosa

    Science.gov (United States)

    Arai, Toshio; Akao, Nobuaki; Seki, Takenori; Kumagai, Takashi; Ishikawa, Hirofumi; Ohta, Nobuo; Hirata, Nobuto; Nakaji, So; Yamauchi, Kenji; Hirai, Mitsuru; Shiratori, Toshiyasu; Kobayashi, Masayoshi; Fujii, Hiroyuki; Ishii, Eiji; Naito, Mikio; Saitoh, Shin-ichi; Yamaguchi, Toshikazu; Shibata, Nobumitsu; Shimo, Masamune; Tokiwa, Toshihiro

    2014-01-01

    Background Anisakiasis is a parasitic disease caused primarily by Anisakis spp. larvae in Asia and in Western countries. The aim of this study was to investigate the genotype of Anisakis larvae endoscopically removed from Middle Eastern Japanese patients and to determine whether mucosal atrophy affects the risk of penetration in gastric anisakiasis. Methods In this study, 57 larvae collected from 44 patients with anisakiasis (42 gastric and 2 colonic anisakiasis) were analyzed retrospectively. Genotyping was confirmed by restriction fragment length polymorphism (RFLP) analysis of ITS regions and by sequencing the mitochondrial small subunit (SSU) region. In the cases of gastric anisakiasis, correlation analyses were conducted between the frequency of larval penetration in normal/atrophic area and the manifestation of clinical symptoms. Results Nearly all larvae were A. simplex seusu stricto (s.s.) (99%), and one larva displayed a hybrid genotype. The A. simplex larvae penetrated normal mucosa more frequently than atrophic area (p = 0.005). Finally, patients with normal mucosa infection were more likely to exhibit clinical symptoms than those with atrophic mucosa infection (odds ratio, 6.96; 95% confidence interval, 1.52–31.8). Conclusions In Japan, A. simplex s.s. is the main etiological agent of human anisakiasis and tends to penetrate normal gastric mucosa. Careful endoscopic examination of normal gastric mucosa, particularly in the greater curvature of the stomach will improve the detection of Anisakis larvae. PMID:24586583

  18. Cytogenetic damage in circulating lymphocytes and buccal mucosa cells of head-and-neck cancer patients undergoing radiotherapy

    International Nuclear Information System (INIS)

    Minicucci, E.M.; Ribeiro, L.R.; Camargo, J.L.V. de; Salvadori, D.M.F.

    2005-01-01

    This study evaluated cytogenetic damage by measuring the frequency of micronucleated cells (MNC) in peripheral blood and buccal mucosa of head-and-neck cancer patients undergoing radiotherapy. MNC frequencies were assessed in 31 patients before, during, and after radiotherapy, and in 17 healthy controls matched for gender, age, and smoking habits. Results showed no statistically significant difference between patients and controls prior to radiotherapy in cytokinesis-blocked lymphocytes or buccal mucosa cells. During treatment, increased MNC frequencies were observed in both cell types. Micronucleated lymphocyte levels remained high in samples collected 30 to 140 days after the end of treatment, while MNC frequency in buccal mucosa decreased to values statistically similar to baseline values. There is controversy over the effects of age, smoking habit, tumor stage, and/or metastasis on MNC frequency. However, increased frequency of micronucleated buccal mucosa cells was seen in patients under 60 years old and in those with tumors >4 cm. In conclusion, the data show that radiotherapy has a potent clastogenic effect in circulating lymphocytes and buccal mucosa cells of head-and-neck cancer patients, and that the baseline MNC frequency in these two tissues is not a sensitive marker for head-and neck neoplasm. (author)

  19. Espesor de la mucosa palatina previo a la colocación de injertos de tejido blando

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    Cynthia Baltodano Ríos

    2016-01-01

    Full Text Available Conocer el espesor de mucosa palatina es de fundamental importancia al momento de realizar procedimientos de autoinjertos de tejido blando en cirugía periodontal. Este estudio tuvo como objetivo determinar el espesor de mucosa palatina en una población peruana joven. Materiales y métodos: Estudio descriptivo que incluyó a 24 pacientes sanos (12 varones y 12 mujeres. A través de una férula de acrílico con 20 puntos de referencia (a nivel de canino, primera premolar, segunda premolar, primera molar y segunda molar fueron realizadas las perforaciones con una sonda periodontal topada. Las medidas fueron registradas a partir de puntos sangrantes a 5 minutos y 10 minutos post inyección anestésica. Las referencias desde el margen gingival fueron registradas a 3mm, 6mm, 9mm y 12mm. Resultados: El espesor de mucosa palatina promedio de todos los pacientes fue de 2,86 ± 0,22mm; el espesor de mucosa de los varones fue de 2,87 ±0,2mm mientras que el espesor de las mujeres fue de 2,85 ± 0,26 no encontrándose diferencias significativas (p=0.9. Conclusión: El espesor de mucosa palatina de la población peruana joven fue mayor en el grupo de los varones y a nivel de la segunda molar superior.

  20. Efficacy of pediatric colonoscopy used as push enteroscopy in the management of capsule endoscopy findings Eficacia del colonoscopio pediátrico como enteroscopio de pulsión en el manejo de los hallazgos de la cápsula endoscópica

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    F. Pérez Roldán

    2009-07-01

    endoscopia nos han permitido explorar de una forma más eficaz el intestino delgado, tanto con la cápsula endoscópica (CE como con enteroscopia de doble balón. El problema que aparece es la no disponibilidad de estos dos métodos en todos los hospitales. Por ello, cuando se ven lesiones en la CE, una alternativa posible para el tratamiento de las lesiones de yeyuno proximal podría ser la realización de una enteroscopia de pulsión con colonoscopio con fines terapéuticos y de marcaje para control de las lesiones. Objetivos: describir la eficacia del colonoscopio pediátrico en cuanto al diagnóstico, señalización de zona explorada y posibilidades terapéuticas. Material y métodos: durante el periodo comprendido entre octubre de 2007 y septiembre de 2008, se han realizado 21 enteroscopias con colonoscopio pediátrico, de las cuales 9 eran para tomar biopsias yeyunales por sospecha de patología mucosa. De las otras 12, en 10 se vieron lesiones por la cápsula endoscópica (malformaciones vasculares o sangrado yeyunal y en 2 se hizo tránsito intestinal sospecha de estenosis yeyunal. Se utilizó un colonoscopio pediátrico PENTAX EC-3470-LK que presenta un diámetro de sección de 11,6 mm y un canal de trabajo de 3,8 mm, lo cual nos permite realizar cualquier tipo de tratamiento endoscópico habitual. Resultados: se hizo enteroscopia con fines terapéuticos en 7 hom-bres y 5 mujeres, con una edad media de 63,3 años. Se vieron lesiones endoscópicas en yeyuno en diez casos: 5 angiodisplasias, 2 estenosis yeyunales, uno con pliegues engrosados yeyunales con histología normal, un tumor submucoso erosionado y un caso de varices duodenales y yeyunales. En los últimos pacientes se hizo marcaje de la zona más distal explorada con tinta china (2 o con hemoclips (4 o con ambos, para ayudar a localizar topográficamente las lesiones mediante radiología simple de abdomen o cápsula endoscópica. Conclusiones: la enteroscopia de yeyuno ha permitido un diagnóstico de

  1. Expression of neuropeptide W in rat stomach mucosa: regulation by nutritional status, glucocorticoids and thyroid hormones.

    Science.gov (United States)

    Caminos, Jorge E; Bravo, Susana B; García-Rendueles, María E R; Ruth González, C; Garcés, Maria F; Cepeda, Libia A; Lage, Ricardo; Suárez, Miguel A; López, Miguel; Diéguez, Carlos

    2008-02-07

    Neuropeptide W (NPW) is a recently identified neuropeptide that binds to G-protein-coupled receptor 7 (GPR7) and 8 (GPR8). In rodent brain, NPW mRNA is confined to specific nuclei in hypothalamus, midbrain and brainstem. Expression of NPW mRNA has also been confirmed in peripheral organs such as stomach. Several reports suggested that brain NPW is implicated in the regulation of energy and hormonal homeostasis, namely the adrenal and thyroid axes; however the precise physiological role and regulation of peripheral NPW remains unclear. In this study, we examined the effects of nutritional status on the regulation of NPW in stomach mucosa. Our results show that in this tissue, NPW mRNA and protein expression is negatively regulated by fasting and food restriction, in all the models we studied: males, females and pregnant females. Next, we examined the effect of glucocorticoids and thyroid hormones on NPW mRNA expression in the stomach mucosa. Our data showed that NPW expression is decreased in this tissue after glucocorticoid treatment or hyperthyroidism. Conversely, hypothyroidism induces a marked increase in the expression of NPW in rat stomach. Overall, these data indicate that stomach NPW is regulated by nutritional and hormonal status.

  2. ORAL MUCOSA DAMAGE BECAUSE OF HYPOCHLORITE ACCIDENT – A CASE REPORT AND LITERATURE REVIEW

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    Elitsa Deliverska

    2016-08-01

    Full Text Available Background Hypochlorite solution is widely used in dental practice during root canal treatment. Although it is generally regarded as being very safe, potentially severe complications can occur when it comes into contact with soft tissue especially due to its cytotoxic features. Objective The aim of our paper is to present a case of damage of oral mucosa because of leakage of 3% hypochlorite through rubber dam during endodontic treatment. Material and methods We present a 31 years old female with necrosis of buccal mucosa during the endodontic treatment of 46. Results Three days after the procedure the patient was referred to our department for consultation and treatment. Antiseptic lavage was performed and oral antibiotic was administrated. After 5 days intraoral examination showed signs of almost full recovery. Conclusion The need for proper tooth isolation during restorative procedures is obvious. Anything that obscures the operative field negatively impacts operator efficiency and effectiveness. Visibility, patient/operator safety, infection control and the physical properties of dental materials are all compromised when proper isolation is lacking.

  3. Early Regenerative Modifications of Human Postmenopausal Atrophic Vaginal Mucosa Following Fractional CO2 Laser Treatment

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    Stefano Salvatore

    2018-01-01

    Full Text Available BACKGROUND: Postmenopausal women experience undesired symptoms that adversely affect their quality of life. In the recent years, a specific 12 - week fractional CO2 laser treatment has been introduced, with highly significant relief of symptoms. AIM: The aim of this paper is the identification of the early modifications of structural components of atrophic vaginal mucosa induced by laser irradiation, which is responsible for the restorative processes. MATERIAL AND METHODS: We investigated by microscopical, ultrastructural and biochemical methods the modifications of the structural components of postmenopausal atrophic vaginal mucosa tissues after 1 hour following a single fractional laser CO2 application. RESULTS: In one hour, the mucosal epithelium thickens, with the maturation of epithelial cells and desquamation at the epithelial surface. In the connective tissue, new papillae indenting the epithelium with newly formed vessels penetrating them, new thin fibrils of collagen III are also formed in a renewed turnover of components due to the increase of metalloproteinase - 2. Specific features of fibroblasts support stimulation of their activity responsible of the renewal of the extracellular matrix, with an increase of mechanical support as connective tissue and stimulation of growth and maturation to epithelium thanks to new vessels and related factors delivered. CONCLUSION: We found the activation of regenerative mechanisms expressed both in the connective tissue - with the formation of new vessels, new papillae, and new collagen - and in the epithelium with the associated thickening and desquamation of cells at the mucosal surface.

  4. Low-dose-rate interstitial brachytherapy preserves good quality of life in buccal mucosa cancer patients

    International Nuclear Information System (INIS)

    Tayier, A.; Hayashi, Keiji; Yoshimura, Ryoichi

    2011-01-01

    The purpose of this study was to determine the results and long-term changes in radiation toxicity of stage I-II buccal mucosa cancer patients treated by low-dose-rate (LDR) brachytherapy with 198 Au grains. A total of 133 stage I-II buccal mucosa carcinomas patients received 198 Au grain implantation brachytherapy between January 1982 and July 2005: 75 of them were treated by 198 Au grain implantation alone and 58 were treated by 198 Au implantation in combination with external irradiation. The average 198 Au-grain dose was 70 Gy in 7 days. Gross tumor areas ranged from 2.4 cm 2 to 9 cm 2 , and the clinical target areas ranged from 6 cm 2 to 15 cm 2 . The follow-up periods ranged from 3 months to 20 years (mean: 5 years 11 months and median: 5 years 1 months). Failure at the site of the primary lesion occurred in 17 patients. Post-treatment mucosal ulceration developed in 15 patients, and all were cured within 25 months by conservative treatment. Osteoradionecrosis was diagnosed in 8 patients, but only one patient required surgical treatment. No severe complications or aggravation of complications developed more than 10 years after treatment. The results of low-dose-rate (LDR)-brachytherapy (BT) alone and LDR-BT in combination with external irradiation at a total dose of 25 Gy were acceptable from the standpoint of cure rate and quality of life (QOL). (author)

  5. An experimental study on tissue reaction of various contrast agents on endometrium, tuber mucosa, and peritoneum

    International Nuclear Information System (INIS)

    Kim, Jae Seung; Kim, Seung Hyup; Park, In Ae; Park, Jae Hyung; Yoon, Dae Young; Yeon, Kyung Mo

    1994-01-01

    To compare the tissue reactions of various water-soluble and oil-based contrast agents on the endometrium, salpingeal mumosa, and peritoneum. Thirty-three rabbits were used for evaluating the histologic reactions of uterine endometrium, salpinx, and peritoneum. Hysterosalpingography(HSG) was underwent in these rabbits by used Lipiodol, Hexabrix, Rayvist. Ultravist-300, Ultravist-370, and normal saline. Pathologic results were obtained in each of the six groups from the uterine endometrium, salpingeal mucosa, and peritoneum without knowledge of the contrast agent used and time interval from HSG. Mild inflammations were observed in the endometrium, salpingeal mucosa, and peritoneum during the first week of HSG in all rabbits in which water-soluble contrast agents were used. Although there was no significant difference in the degree of inflammation among the groups using various contrast agents, the group with oil-based contrast agent(Lipiodol) showed delayed absorption of contrast agent in the peritoneum, frequent intravasation, fat granuloma, peritoneal adhesion, or uterine infarction. Our results suggest that water-soluble contrast agents can be used safely for HSG, but the use of oil-based contrast agent is questional in safety and should be avoid in patients with tubal obstruction, salpingitis, or endometritis

  6. The role of Mycobacterium avium complex fibronectin attachment protein in adherence to the human respiratory mucosa.

    Science.gov (United States)

    Middleton, A M; Chadwick, M V; Nicholson, A G; Dewar, A; Groger, R K; Brown, E J; Wilson, R

    2000-10-01

    Mycobacterium avium complex (MAC) are opportunistic respiratory pathogens that infect non-immunocompromised patients with established lung disease, although they can also cause primary infections. The ability to bind fibronectin is conserved among many mycobacterial species. We have investigated the adherence of a sputum isolate of MAC to the mucosa of organ cultures constructed with human tissue and the contribution of M. avium fibronectin attachment protein (FAP) to the process. MAC adhered to fibrous, but not globular mucus, and to extracellular matrix (ECM) in areas of epithelial damage, but not to intact extruded cells and collagen fibres. Bacteria occasionally adhered to healthy unciliated epithelium and to cells that had degenerated exposing their contents, but never to ciliated cells. The results obtained with different respiratory tissues were similar. Two ATCC strains of MAC gave similar results. There was a significant reduction (P fibrous mucus was unchanged. Immunogold labelling demonstrated fibronectin in ECM as well as in other areas of epithelial damage, but only ECM bound FAP. A Mycobacterium smegmatis strain had the same pattern of adherence to the mucosa as MAC. When the FAP gene was deleted, the strain demonstrated reduced adherence to ECM, and adherence was restored when the strain was transfected with an M. avium FAP expression construct. We conclude that MAC adheres to ECM in areas of epithelial damage via FAP and to mucus with a fibrous appearance via another adhesin. Epithelial damage exposing ECM and poor mucus clearance will predispose to MAC airway infection.

  7. Nicotine permeability across the buccal TR146 cell culture model and porcine buccal mucosa in vitro

    DEFF Research Database (Denmark)

    Nielsen, Hanne Mørck; Rassing, Margrethe Rømer

    2002-01-01

    The present study was conducted to investigate and compare the effect of pH and drug concentration on nicotine permeability across the TR146 cell culture model and porcine buccal mucosa in vitro. As a further characterization of the TR146 cell culture model, it was explored whether the results were...... comparable for bi-directional and uni-directional transport in the presence of a transmembrane pH gradient. Nicotine concentrations between 10(-5) and 10(-2) M were applied to the apical side of the TR146 cell culture model or the mucosal side of porcine buccal mucosa. Buffers with pH values of 5.5, 7.......4 and 8.1 were used to obtain different fractions of non- and mono-ionized nicotine. The apparent permeability (P(app)) of nicotine across both models increased significantly with increasing pH, and the P(app) values obtained with the two models could be correlated in a linear manner. With increasing...

  8. Therapeutic interaction of systemically-administered mesenchymal stem cells with peri-implant mucosa.

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    Ryosuke Kondo

    Full Text Available OBJECTIVES: The objective of this study was to investigate the effect of systemically transplanted mesenchymal stem cells (MSCs on the peri-implant epithelial sealing around dental implants. MATERIALS AND METHODS: MSCs were isolated from bone marrow of donor rats and expanded in culture. After recipient rats received experimental titanium dental implants in the bone sockets after extraction of maxillary right first molars, donor rat MSCs were intravenously transplanted into the recipient rats. RESULTS: The injected MSCs were found in the oral mucosa surrounding the dental implants at 24 hours post-transplantation. MSC transplantation accelerated the formation of the peri-implant epithelium (PIE-mediated mucosa sealing around the implants at an early stage after implantation. Subsequently, enhanced deposition of laminin-332 was found along the PIE-implant interface at 4 weeks after the replacement. We also observed enhanced attachment and proliferation of oral mucous epithelial cells. CONCLUSION: Systemically transplanted MSCs might play a critical role in reinforcing the epithelial sealing around dental implants.

  9. [The influence of corvitin on secretory processes and blood flow in the rat gastric mucosa].

    Science.gov (United States)

    Vovkun, T V; Ianchuk, P I; Shtanova, L Ia; Vesel'skyĭ, S P; Baranovs'kyĭ, V A

    2013-01-01

    We studied parameters of gastric secretion in pylorus-ligated rat and blood flow in the rat gastric mucosa under the influence of drug corvitin used intragastrically in doses of 2.5 and 5 mg/kg. Biochemical analysis of gastric juice was based on the determination of pH, total hydrochloric acid production and total protein, hexosamine and cysteine concentration. Gastric juice analysis in control rats found the presence of hexosamines-- a gastric mucus indicators and cysteine--free amino acid whith properties of a strong antioxidant. Concentration of these compounds in the gastric juice increased as a consequence of corvitin action. However, corvitin did not affect at these parameters of gastric secretion as the volume of gastric juice, pH, hydrochloric acid output rate, protein concentration. Additionally it was shown that corvitin in dose-dependent manner increased blood flow in the gastric mucosa. This results give reason to believe that corvitin can be considered as a tool that amplifies gastric mucosal defense mechanisms without affecting the secretion of gastric hydrochloric acid and total protein.

  10. Cultured fibroblasts from alveolar and gingival mucosae are biologically and biochemically different

    International Nuclear Information System (INIS)

    Lanz, J.; Banes, A.

    1986-01-01

    Tissues removed from the alveolar or gingival mucosa of 5 patients were separated into cell populations to assess the relative contributions each might make in wound healing intraorally. Growth curves and protein synthetic patterns of fibroblasts, free of epithelial cells, were obtained at pass 5. The morphologies of the two cell types were not grossly different. However, the AM cells (alveolar mucosa) had a generation time (gt) of 18.7 hrs. whereas the gt for KG cells (keratinized gingiva) was 49.6 hrs. Cells labeled in vitro with 35 S-methionine had distinct patterns of protein synthesis. The AM cells had more of the 275, 220, 92, 80, 50 and 46 kd bands on the autoradiogram of a 7.5% PAGE slab gel than did the KG cells. The KG cells contained more of the 165, 84, 68, 60, 54, 51, 43, 36, and 32a kd bands. In a wound healing situation, the AM cells may be the first fibroblasts to rapidly divide to fill a defect, whereas the KG cells may require a longer time period to divide. This is the first report of biochemical and biological differences in these two fibroblast populations from cultured, human tissues

  11. Urokinase and the intestinal mucosa: evidence for a role in epithelial cell turnover

    Science.gov (United States)

    Gibson, P; Birchall, I; Rosella, O; Albert, V; Finch, C; Barkla, D; Young, G

    1998-01-01

    Background—The functions of urokinase in intestinal epithelia are unknown. 
Aims—To determine the relation of urokinase expressed by intestinal epithelial cells to their position in the crypt-villus/surface axis and of mucosal urokinase activity to epithelial proliferative kinetics in the distal colon. 
Methods—Urokinase expression was examined immunohistochemically in human intestinal mucosa. Urokinase activity was measured colorimetrically in epithelial cells isolated sequentially from the crypt-villus axis of the rat small intestine. In separate experiments, urokinase activity and epithelial kinetics (measured stathmokinetically) were measured in homogenates of distal colonic mucosa of 14 groups of eight rats fed diets known to alter epithelial turnover. 
Results—From the crypt base, an ascending gradient of expression and activity of urokinase was associated with the epithelial cells. Median mucosal urokinase activities in each of the dietary groups of rats correlated positively with autologous median number of metaphase arrests per crypt (r=0.68; p<0.005) and per 100 crypt cells (r=0.75; p<0.001), but not with crypt column height. 
Conclusions—Localisation of an enzyme capable of leading to digestion of cell substratum in the region where cells are loosely attached to their basement membrane, and the association of its activity with indexes of cell turnover, suggest a role for urokinase in facilitating epithelial cell loss in the intestine. 

 Keywords: urokinase; intestinal epithelium; colon; epithelial proliferation PMID:9824347

  12. Subepithelial myofibroblasts are novel nonprofessional APCs in the human colonic mucosa.

    Science.gov (United States)

    Saada, Jamal I; Pinchuk, Irina V; Barrera, Carlos A; Adegboyega, Patrick A; Suarez, Giovanni; Mifflin, Randy C; Di Mari, John F; Reyes, Victor E; Powell, Don W

    2006-11-01

    The human gastrointestinal mucosa is exposed to a diverse normal microflora and dietary Ags and is a common site of entry for pathogens. The mucosal immune system must respond to these diverse signals with either the initiation of immunity or tolerance. APCs are important accessory cells that modulate T cell responses which initiate and maintain adaptive immunity. The ability of APCs to communicate with CD4+ T cells is largely dependent on the expression of class II MHC molecules by the APCs. Using immunohistochemistry, confocal microscopy, and flow cytometry, we demonstrate that alpha-smooth muscle actin(+), CD90+ subepithelial myofibroblasts (stromal cells) constitutively express class II MHC molecules in normal colonic mucosa and that they are distinct from professional APCs such as macrophages and dendritic cells. Primary isolates of human colonic myofibroblasts (CMFs) cultured in vitro were able to stimulate allogeneic CD4+ T cell proliferation. This process was dependent on class II MHC and CD80/86 costimulatory molecule expression by the myofibroblasts. We also demonstrate that CMFs, engineered to express a specific DR4 allele, can process and present human serum albumin to a human serum albumin-specific and DR4 allele-restricted T cell hybridoma. These studies characterize a novel cell phenotype which, due to its strategic location and class II MHC expression, may be involved in capture of Ags that cross the epithelial barrier and present them to lamina propria CD4+ T cells. Thus, human CMFs may be important in regulating local immunity in the colon.

  13. Intestinal mucosa in diabetes: synthesis of total proteins and sucrase-isomaltase

    International Nuclear Information System (INIS)

    Olsen, W.A.; Perchellet, E.; Malinowski, R.L.

    1986-01-01

    The effects of insulin deficiency on nitrogen metabolism in muscle and liver have been extensively studied with recent in vivo demonstration of impaired protein synthesis in rats with streptozotocin-induced diabetes. Despite the significant contribution of small intestinal mucosa to overall protein metabolism, the effect of insulin deficiency on intestinal protein synthesis have not been completely defined. The authors studied the effects of streptozotocin-induced diabetes on total protein synthesis by small intestinal mucosa and on synthesis of a single enzyme protein of the enterocyte brush-border membrane sucrase-isomaltase. They used the flood-dose technique to minimize the difficulties of measuring specific radioactivity of precursor phenylalanine and determined incorporation into mucosal proteins and sucrase-isomaltase 20 min after injection of the labeled amino acid. Diabetes did not alter mucosal mass as determined by weight and content of protein and DNA during the 5 days after injection of streptozotocin. Increased rates of sucrase-isomaltase synthesis developed beginning on day 3, and those of total protein developed on day 5. Thus intestinal mucosal protein synthesis is not an insulin-sensitive process

  14. [Efficacy of retained rectal posterior mucosa in procedure for prolapse and hemorrhoids].

    Science.gov (United States)

    Zheng, Chenguo; Jin, Chun; Lian, Shaoxiong; Jin, Dingguo

    2014-12-01

    To evaluate the efficacy and necessity of retained rectal posterior mucosa in procedure for prolapse and hemorrhoids (PPH). Clinical data of 260 cases with severe hemorrhoids in our hospital from January 2010 to May 2012 were analyzed retrospectively. A total of 132 cases with severe hemorrhoids excluding in rectal posterior wall were enrolled in retained rectal posterior mucosa in PPH (improvement group), other 128 cases of severe hemorrhoids were assigned to PPH (conventional group). Operative parameters, efficacy and complication after operation were compared. Two groups of patients received successful operations. Postoperative pain duration, frequency of analgesic drugs and postoperative hospital stay in improvement group were significantly reduced [(1.3 ± 0.5) d vs. (4.8 ± 0.7) d, 1.1 ± 0.3 vs. 5.9 ± 0.6, (5.2 ± 0.8) d vs. (5.8 ± 0.5) d, all Phemorrhoids excluding in rectal posterior wall can significantly reduce postoperative complications. But long-term efficacy needs further observation.

  15. Tratamento da forma mucosa de leishmaniose sem resposta a glucantime, com anfotericina B liposomal

    Directory of Open Access Journals (Sweden)

    Raimunda Nonata Ribeiro Sampaio

    1997-04-01

    Full Text Available Tratamos com ambisome (2 a 5g totais de dose seis pacientes com leishmaniose mucosa sem resposta a tratamento com glucantime (20mg SbV/kg/dia. A dose diária usada foi 2 a 3mg/kg/dia, aplicada por um mínimo de 20 dias. Após 26 a 38 meses de acompanhamento, cinco pacientes estão clinicamente curados. Um recidivou aos 6 meses. Não foram observados efeitos colaterais além de cefaléia, após a injeção. O ambisome constitue uma opção terapêutica para os pacientes com leishmaniose mucosa sem resposta aos antimoniais.We treated six patients with mucosal leishmaniasis who failed to respond to glucantime (20mg/kg/day with ambisome (2-5 grams total dose. The daily dose was 2-3mg/kg/day given for a minimum of 20 days. After 26-38 months of follow up, five patients were clinically cured. One relapsed after six months. No side effects of therapy were observed apart from headache after injection. Ambisome is a therapeutic option for patients with mucosal leishmaniasis unresponsive to antimonials.

  16. Buccal mucosa carcinoma: surgical margin less than 3 mm, not 5 mm, predicts locoregional recurrence

    Directory of Open Access Journals (Sweden)

    Chiou Wen-Yen

    2010-09-01

    Full Text Available Abstract Background Most treatment failure of buccal mucosal cancer post surgery is locoregional recurrence. We tried to figure out how close the surgical margin being unsafe and needed further adjuvant treatment. Methods Between August 2000 and June 2008, a total of 110 patients with buccal mucosa carcinoma (25 with stage I, 31 with stage II, 11 with stage III, and 43 with Stage IV classified according to the American Joint Committee on Cancer 6th edition were treated with surgery alone (n = 32, surgery plus postoperative radiotherapy (n = 38 or surgery plus adjuvant concurrent chemoradiotherapy (n = 40. Main outcome measures: The primary endpoint was locoregional disease control. Results The median follow-up time at analysis was 25 months (range, 4-104 months. The 3-year locoregional control rates were significantly different when a 3-mm surgical margin (≤3 versus >3 mm, 71% versus 95%, p = 0.04 but not a 5-mm margin (75% versus 92%, p = 0.22 was used as the cut-off level. We also found a quantitative correlation between surgical margin and locoregional failure (hazard ratio, 2.16; 95% confidence interval, 1.14 - 4.11; p = 0.019. Multivariate analysis identified pN classification and surgical margin as independent factors affecting disease-free survival and locoregional control. Conclusions Narrow surgical margin ≤3 mm, but not 5 mm, is associated with high risk for locoregional recurrence of buccal mucosa carcinoma. More aggressive treatment after surgery is suggested.

  17. Buccal mucosa carcinoma: surgical margin less than 3 mm, not 5 mm, predicts locoregional recurrence

    International Nuclear Information System (INIS)

    Chiou, Wen-Yen; Hung, Shih-Kai; Lin, Hon-Yi; Hsu, Feng-Chun; Lee, Moon-Sing; Ho, Hsu-Chueh; Su, Yu-Chieh; Lee, Ching-Chih; Hsieh, Chen-Hsi; Wang, Yao-Ching

    2010-01-01

    Most treatment failure of buccal mucosal cancer post surgery is locoregional recurrence. We tried to figure out how close the surgical margin being unsafe and needed further adjuvant treatment. Between August 2000 and June 2008, a total of 110 patients with buccal mucosa carcinoma (25 with stage I, 31 with stage II, 11 with stage III, and 43 with Stage IV classified according to the American Joint Committee on Cancer 6 th edition) were treated with surgery alone (n = 32), surgery plus postoperative radiotherapy (n = 38) or surgery plus adjuvant concurrent chemoradiotherapy (n = 40). Main outcome measures: The primary endpoint was locoregional disease control. The median follow-up time at analysis was 25 months (range, 4-104 months). The 3-year locoregional control rates were significantly different when a 3-mm surgical margin (≤3 versus >3 mm, 71% versus 95%, p = 0.04) but not a 5-mm margin (75% versus 92%, p = 0.22) was used as the cut-off level. We also found a quantitative correlation between surgical margin and locoregional failure (hazard ratio, 2.16; 95% confidence interval, 1.14 - 4.11; p = 0.019). Multivariate analysis identified pN classification and surgical margin as independent factors affecting disease-free survival and locoregional control. Narrow surgical margin ≤3 mm, but not 5 mm, is associated with high risk for locoregional recurrence of buccal mucosa carcinoma. More aggressive treatment after surgery is suggested

  18. Averaged subtracted polarization imaging for endoscopic diagnostics of surface microstructures on translucent mucosae

    Science.gov (United States)

    Kanamori, Katsuhiro

    2016-07-01

    An endoscopic image processing technique for enhancing the appearance of microstructures on translucent mucosae is described. This technique employs two pairs of co- and cross-polarization images under two different linearly polarized lights, from which the averaged subtracted polarization image (AVSPI) is calculated. Experiments were then conducted using an acrylic phantom and excised porcine stomach tissue using a manual experimental setup with ring-type lighting, two rotating polarizers, and a color camera; better results were achieved with the proposed method than with conventional color intensity image processing. An objective evaluation method that uses texture analysis was developed and used to evaluate the enhanced microstructure images. This paper introduces two types of online, rigid-type, polarimetric endoscopic implementations using a polarized ring-shaped LED and a polarimetric camera. The first type uses a beam-splitter-type color polarimetric camera, and the second uses a single-chip monochrome polarimetric camera. Microstructures on the mucosa surface were enhanced robustly with these online endoscopes regardless of the difference in the extinction ratio of each device. These results show that polarimetric endoscopy using AVSPI is both effective and practical for hardware implementation.

  19. Regeneration of the intestinal mucosa in Eimeria and E. Coli challenged broilers supplemented with amino acids.

    Science.gov (United States)

    Gottardo, E T; Prokoski, K; Horn, D; Viott, A D; Santos, T C; Fernandes, J I M

    2016-05-01

    The aim of this study was to evaluate the regeneration of the intestinal mucosa in Eimeria and E. coli challenged broilers supplemented with glutamine, arginine, and threonine. Six hundred male broilers at one d of age from the Cobb strain were utilized. The design was completely randomized using a 2×3 factorial design (unchallenged and challenged and 3 diets). A commercial diet was used as a control and 2 other diets were formulated with glutamine (1.5 and 3% Aminogut®), arginine (1 and 2% L-Arginine), and threonine (1 and 2% L-threonine). The animals that consumed diets supplemented with amino acids presented better (Pbroilers that received diets supplemented with amino acids. High levels of amino acids in the experimental feeds reflected in greater protein levels in poultry house litter, and they did not interfere with ammonia production. The supplementation of diets with trophic amino acids can positively contribute to the regeneration and proliferation of the intestinal mucosa in broilers and to the maintenance of zootechnical performance when submitted to enteric challenges. © 2016 Poultry Science Association Inc.

  20. A case of primary mucosa-associated lymphoid tissue lymphoma of the vagina.

    Science.gov (United States)

    Yoshinaga, Kousuke; Akahira, Jun-Ichi; Niikura, Hitoshi; Ito, Kiyoshi; Moriya, Takuya; Murakami, Takashi; Kameoka, Jun-Ichi; Ichinohasama, Ryo; Okamura, Kunihiro; Yaegashi, Nobuo

    2004-09-01

    We report the first case of primary mucosa-associated lymphoid tissue (MALT) lymphoma of the vagina, the diagnosis of which is supported by genetic and immunophenotypic studies. A 65-year-old, para 2 woman presented to our hospital in July 1997 with a history of prolonged vaginal discharge. Although cytologic examination suggested possible malignancy, a biopsy of the vaginal wall was diagnosed as chronic inflammation. In June 2000, she underwent gynecologic examination because of anuria. Excisional biopsy revealed subepithelial infiltration of atypical lymphoid cells that stained for CD20, CD79a, and BCL-2; stained weakly for IgM; and did not stain for CD3, CD5, CD7, CD10, CD56, CD23, and IgD, suggesting marginal zone B-cell lineage. Monoclonality was detected by Southern blot analysis, and this patient was finally diagnosed as having primary MALT lymphoma of the vagina. She received 3 cycles of chemotherapy (THP-COP) and concurrent radiation to the whole pelvis. The patient is alive and well 40 months after treatment. Because the vagina is one of the mucosa-associated tissues, MALT lymphoma, though rare, must be included in the differential diagnosis of the vaginal neoplasms.

  1. Scap is required for sterol synthesis and crypt growth in intestinal mucosa.

    Science.gov (United States)

    McFarlane, Matthew R; Cantoria, Mary Jo; Linden, Albert G; January, Brandon A; Liang, Guosheng; Engelking, Luke J

    2015-08-01

    SREBP cleavage-activating protein (Scap) is an endoplasmic reticulum membrane protein required for cleavage and activation of sterol regulatory element-binding proteins (SREBPs), which activate the transcription of genes in sterol and fatty acid biosynthesis. Liver-specific loss of Scap is well tolerated; hepatic synthesis of sterols and fatty acids is reduced, but mice are otherwise healthy. To determine whether Scap loss is tolerated in the intestine, we generated a mouse model (Vil-Scap(-)) in which tamoxifen-inducible Cre-ER(T2), a fusion protein of Cre recombinase with a mutated ligand binding domain of the human estrogen receptor, ablates Scap in intestinal mucosa. After 4 days of tamoxifen, Vil-Scap(-) mice succumb with a severe enteropathy and near-complete collapse of intestinal mucosa. Organoids grown ex vivo from intestinal crypts of Vil-Scap(-) mice are readily killed when Scap is deleted by 4-hydroxytamoxifen. Death is prevented when culture medium is supplemented with cholesterol and oleate. These data show that, unlike the liver, the intestine requires Scap to sustain tissue integrity by maintaining the high levels of lipid synthesis necessary for proliferation of intestinal crypts. Copyright © 2015 by the American Society for Biochemistry and Molecular Biology, Inc.

  2. Metabolism of model organic pollutants in canine respiratory tract mucosa slices

    International Nuclear Information System (INIS)

    Thornton-Manning, J.R.; Gerde, P.; Chen, S.T.; Dahl, A.R.

    1994-01-01

    The high incidence of human bronchial tumors has been correlated with the high fractional deposition of inhaled particles in the bronchi. Polycyclic aromatic hydrocarbons (PAHs) are frequently bound to airborne particles due to their low vapor pressures. It is thought that tumorigenicity may result from the release and subsequent bioactivation of these particle-associated organic compounds in the respiratory tract. Previous studies at ITRI examined the clearance of organic toxicants from various regions of the canine respiratory tract. Their results indicated that, while clearance of a highly lipophilic PAH such as benzo(a)pyrene (BaP) from the thin alveolar epithelium took only a few minutes, clearance through the thicker epithelium of the conducting airways took hours. Slower, diffusion-limited clearance results in higher concentrations of lipophilic compounds in the epithelium of the bronchi. Hence, the ability of these tissues to metabolize organic compounds to water-soluble metabolites or reactive intermediates may be extremely important in their clearance from the respiratory tract and the potential susceptibility of this region of the respiratory tract to cancer. The purpose of the present study was to evaluate the ability of bronchial mucosa to metabolize a model organic pulmonary carcinogen, BaP, to reactive and nonreactive metabolites and to evaluate the diffusion of the parent compound and metabolites through the bronchial mucosa

  3. Radioautographic analysis of changes in different phases of cell kinetics in murine oral mucosa

    International Nuclear Information System (INIS)

    Park, Chang Suck; You, Dong Soo

    1983-01-01

    The age related changes in the life cycle of the progenitor cell population of murine oral epithelia was studied. Using radioautographic methods which have been adopted in previous cell cycle studies, the age-related changes of different phases in renewing cells of the palatal, buccal and lingual mucosae were determined. The results confirm published findings on cell cycle changes of epithelia with aging and illustrated further that mitotic phases which has hither to been considered stationary, also changes with aging. The major parts revealed by this study are as follows: 1. The basal progenitor cells in different regions of oral mucosa have different generation times. 2. The basal cell cycle time increases as a function of aging and the region most affected by aging appears to be the epithelium of the cheek. 3. The phases of the cell cycle affected by the process of aging are in increasing order of magnitude: M-, S- and G1-phase. 4. The age related change in the number of DNA synthesizing basal progenitor cells occurs at two age periods. Between 1 and 12 months of life it decreases, while from 12 to 20 months it increases.

  4. Pulmonary infiltration with eosinophilia complicated with mucosa-associated lymphoid tissue lymphoma: A case report.

    Science.gov (United States)

    Liu, Yin; Tangsun, Yinyan; Xiao, Yonglong; Zhang, Deping; Cao, Min

    2016-09-01

    Tissue eosinophilia is rarely observed in cases of non-Hodgkin's lymphoma of B cell origin. The present study describes a rare case of mucosa-associated lymphoid tissue (MALT) lymphoma, which was initially misdiagnosed as eosinophilic pneumonia. The initial diagnosis was formed based on the results of chest radiography, peripheral eosinophilia tests and bronchoalveolar lavage, and the clinical course of the patient. Following administration of methylprednisolone (40 mg/day) for 4 days and oral administration of prednisolone (30 mg/day), the clinical course rapidly improved and the eosinophil count immediately decreased a to normal level. However, abnormal shadows observed on computed tomography (CT) scans of the chest did not diminish. At 6 months after the initiation of treatment, CT-guided percutaneous lung biopsy was performed, and a final diagnosis of primary pulmonary mucosa-associated lymphoid tissue lymphoma was made based on immunohistochemical examination. Primary lung MALT lymphoma remains a rare entity, with an indolent course and a reasonably favorable prognosis, whose diagnosis may be challenging.

  5. High Expression of Antiviral Proteins in Mucosa from Individuals Exhibiting Resistance to Human Immunodeficiency Virus.

    Directory of Open Access Journals (Sweden)

    Sandra Milena Gonzalez

    Full Text Available Several soluble factors have been reported to have the capacity of inhibiting HIV replication at different steps of the virus life cycle, without eliminating infected cells and through enhancement of specific cellular mechanisms. Yet, it is unclear if these antiviral factors play a role in the protection from HIV infection or in the control of viral replication. Here we evaluated two cohorts: i one of 58 HIV-exposed seronegative individuals (HESNs who were compared with 59 healthy controls (HCs, and ii another of 13 HIV-controllers who were compared with 20 HIV-progressors. Peripheral blood, oral and genital mucosa and gut-associated lymphoid tissue (GALT samples were obtained to analyze the mRNA expression of ELAFIN, APOBEC3G, SAMHD1, TRIM5α, RNase 7 and SerpinA1 using real-time PCR.HESNs exhibited higher expression of all antiviral factors in peripheral blood mononuclear cells (PBMCs, oral or genital mucosa when compared with HCs. Furthermore, HIV-controllers exhibited higher levels of SerpinA1 in GALT.These findings suggest that the activity of these factors is compartmentalized and that these proteins have a predominant role depending on the tissue to avoid the infection, reduce the viral load and modulate the susceptibility to HIV infection.

  6. MicroRNA-130a is highly expressed in the esophageal mucosa of achalasia patients.

    Science.gov (United States)

    Shoji, Hiroyuki; Isomoto, Hajime; Yoshida, Akira; Ikeda, Haruo; Minami, Hitomi; Kanda, Tsutomu; Urabe, Shigetoshi; Matsushima, Kayoko; Takeshima, Fuminao; Nakao, Kazuhiko; Inoue, Haruhiro

    2017-08-01

    Esophageal achalasia is considered as a risk factor of esophageal cancer. The etiologies of esophageal achalasia remain unknown. Peroral endoscopic myotomy (POEM) has recently been established as a minimally invasive method with high curability. The aims of the present study were to identify the microRNAs (miRs) specific to esophageal achalasia, to determine their potential target genes and to assess their alteration following POEM. RNA was extracted from biopsy samples from middle esophageal mucosa and analyzed using a microarray. Differentially expressed miRs in achalasia patients compared with control samples were identified and analyzed using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Correlations between specific miR expression levels and the patients' clinical background were also investigated. In addition, alterations of selected miR expression levels before and after POEM were analyzed. The results of RT-qPCR analysis demonstrated that the miR-130a expression levels were significantly higher in patients with achalasia (Pachalasia. However, no significant change in miR-130a expression was observed between before and after POEM. In conclusion, miR-130a is highly expressed in the esophageal mucosa of patients with achalasia and may be a biomarker of esophageal achalasia.

  7. Epstein-Barr virus in oral mucosa from human immunodeficiency virus positive patients

    Directory of Open Access Journals (Sweden)

    Larissa Santos

    2014-06-01

    Full Text Available Objective: the detection rate of Epstein-Barr virus (EBV is higher in people living with human immunodeficiency virus (HIV. In an attempt to contribute to our epidemiological understanding of this coinfection and to investigate the activity of EBV in normal oral mucosa, we performed a cross-sectional study with HIV-positive patients. Methods: oral smears from 145 HIV-positive patients were collected between March 2010 and March 2011. Nested polymerase chain reaction (PCR and reverse transcriptase-PCR (RT-PCR were used to genotype EBV and to detect EBNA-2 expression, respectively. Results: EBV DNA was detected in 48.3% of the study participants, of whom 32.85% were EBV-1 and 45.71% were EBV-2 carriers. Additionally, 14.28% were coinfected with both types. EBNA-2 mRNA was expressed in 45.7% of the EBV -positive samples, including 20.0% with EBV-1 only, 20.0% with EBV-2 only and 1.4% with both genotypes. Immune status affected the overall EBV infection, and EBV-2 positivity was significantly correlated with sexual lifestyle of the participants. EBV co-infection with both viral types was dependent upon HIV viral load and the activity of the EBNA-2 gene. Conclusion: we report a high prevalence of active EBV in the oral mucosa of asymptomatic HIV-seropositive individuals. This study addresses the need for monitoring and treatment of HIV-infected patients with EBV reactivation.

  8. Effects of feed additives on ileal mucosa-associated microbiota composition of broiler chickens.

    Science.gov (United States)

    Ruiz, R; Peinado, M J; Aranda-Olmedo, I; Abecia, L; Suárez-Pereira, E; Ortiz Mellet, C; García Fernández, J M; Rubio, L A

    2015-07-01

    The effects of dietary supplementation with 2 recently developed feed additives on the composition of the mucosa-associated microbiota of the ileum were studied in growing broiler chickens. A total of 48 male 1-d-old broiler chickens of the Cobb 500 strain were distributed in 4 treatments with 2 replicates of 6 birds each. The 2 additives tested were a di-d-fructose dianhydride–enriched caramel (FC) and the garlic derivative propyl propane thiosulfonate (PTS-O). Dietary treatments were a control (commercial diet with no additive), INU (20 g inulin/kg diet), CAR (20 g FC/kg diet), and GAR (90 mgPTS-O/kg diet). As a result of this study, inulin supplementation resulted in lower (P Eubacterium rectale log10 number of copies respect to controls. Higher (P spp. revealed the presence of Bifidobacterium longum, Bifidobacterium pseudolongum, and Bifidobacterium pseudocatenulatum in samples from chickens fed the control and the PTS-O–supplemented diet. Bifidobacterium longum was exclusively found in poultry fed the control diet, whereas B. pseudocatenulatum was found only in poultry fed the PTS-O–supplemented diet. This study showed that both PTS-O and FC were able to modulate the composition of the ileal mucosa-associated microbiota of growing broiler chickens. Finally, in addition to B. pseudolongum, the presence of B. longum and B. pseudocatenulatum, species not previously described in intestinal samples of broilers, was also demonstrated.

  9. Penetration effect of prostaglandin E2 gel on oral mucosa of rats

    Directory of Open Access Journals (Sweden)

    Rafinus Arifin

    2012-09-01

    Full Text Available Background: Several researches reported that Prostaglandin E2 (PGE2 injection on buccal mucosa combined with orthodontic pressure can faster tooth movement but has disadvantages such as high alveolar bone and root resorption furthermore pain from injection needle. PGE2 gel was made to better replace the lacks of injectable PGE2. Purpose: This research was aimed to prove that PGE 2 gel can penetrate rat’s oral mucosa effecting the appearance of PMN cells. Methods: This research was an in vivo laboratory experiment using 36 Sprague Dawley rats which were divided into 3 groups: normal group, topical PGE2 gel group after 1, 2, 4, 8 hours (4 subgroups, and topical gel without PGE2 group after 1, 2, 4, 8 hours (4 subgroups. Each group consists of 4 rats, therefore the total sample for all research groups were 36 rats. Gel with 25 µg/mL of PGE2 and gel without PGE2 were applied on oral mucosa for 2 minutes. Then, the rats were sacrificed after 1 hour, 2 hours, 4 hours, and 8 hours application. After that, the samples were prepared for histological examination with Hematoxyllin and Eosin. The picture were taken with OptiLab View and PMN cells amount were counted with light microscope, set 400 times of magnification. Results: Penetration effect of PGE2 gel on rat’s oral mucosa result in PMN inflammation cells distribution. One-way ANOVA showed no significant difference on PMN cells count in rats’ lower jaws between groups of normal and gel without PGE2. There was significant difference between groups of PGE2 gel and gel without PGE2 (p=0,001. PGE2 gel application showed PGE2 as inflammatory media, even though administered topically. Conclusion: PGE2 gel can penetrate rat’s oral mucosa, effecting PMN cells 1, 2, 4 and 8 hours after application of PGE2 gel.Latar belakang: Beberapa penelitian melaporkan bahwa injeksi (Prostaglandin E2 PGE2pada mukosa bukal yang dikombinasikan dengan tekanan ortodonti dapat mempercepat pergerakan gigi, tapi

  10. Estudio histológico del sistema digestivo en diferentes estadios de desarrollo de la cachama blanca (Piaractus brachypomus

    Directory of Open Access Journals (Sweden)

    Miguel Ángel Mendoza R.

    2013-06-01

    Full Text Available La cachama blanca (Piaractus brachypomus es la segunda fuente de producción piscícola en Colombia, después de la tilapia roja (Oreochomi ssp. y antes de la trucha arcoíris (Oncorhynchus mykiss. Se destaca, además, como una de las especies con mayor potencial productivo en el país. Se realizó el estudio histológico del sistema digestivo de la Piaractus brachypomus a fin determinar la morfología normal en diferentes estadios de desarrollo. Para ello se clasificaron los animales según la edad, el tamaño y el peso en ovas embrionadas, larvas con saco vitelino, alevinos después de reabsorción de saco vitelino, juveniles (3-5 cm, 5-7 cm, 10 cm, 15 cm y adultos (300 g. La fijación de los tejidos se realizó en formaldehído al 3,7 % y se describió microscópicamente cavidad bucal, faringe, esófago, estómago, ciegos pilóricos, intestinos y las glándulas anexas, hígado y páncreas. Por último, se describió macroscópicamente la organización anatómica de este sistema.

  11. Does site of buccal mucosa graft for bulbar urethra stricture affect outcome? A comparative analysis of ventral, dorso-lateral and dorsal buccal mucosa graft augmentation urethroplasty.

    Science.gov (United States)

    Pathak, Hemant R; Jain, Tarunkumar Prakash; Bhujbal, Sachin A; Meshram, Kunal R; Gadekar, Chetan; Parab, Sandesh

    2017-09-01

    To compare long- term outcomes of buccal mucosa graft (BMG) augmentation urethroplasty for long segment bulbar urethral strictures done by placing the graft ventrally, dorso-laterally and dorsally. We conducted a single institution retrospective study on 112 who underwent BMG augmentation urethroplasty for non-traumatic bulbar urethral strictures between January 2005 to December 2014. The cases were divided into three groups based on the site of placement of BMG graft i.e. (a) Ventral (n=44), (b) Dorso-lateral (n=48) and (c) Dorsal (n=20). Follow-up period was from one year to five years. Patients with failed outcomes underwent urethroscopy or retrograde urethrogram to note the site of recurrence of stricture. Out of 112 cases 91 (81%) were successful and 21 (19%) failed. The success rates for ventral, dorso-lateral and dorsal BMG augmentation procedures were 89%, 79% and 70%, respectively (p=0.18). Among 21 failed cases, 12 cases (57%) had stricture at proximal anastomotic site, 4 cases (19%) at graft and 5 cases (24%) at distal anastomotic site (p=0.01). The overall success rate for BMG augmentation urethroplasty is equal for all techniques. Ventral onlay urethroplasty provides better exposure of proximal anastomotic site thus it is associated with minimum proximal anastomotic site recurrence rates. Patients with extensive spongiofibrosis and long segment strictures had higher rates of failure.

  12. Morphofunctional State of Esophageal Mucosa in Patients with Gastroesophageal Reflux Disease Depending on the Nutritional Status of Patients

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    H.D. Fadieienko

    2015-11-01

    Full Text Available We have studied morphofunctional state of esophageal mucosa in 57 patients with gastroesophageal reflux disease (GERD with normal (body mass index (BMI — 18.5–24.9 kg/m2 and excessive (BMI over 25 kg/m2 body weight. A histologic and morphometric study of esophageal mucosa, immunohistochemical analysis of the expression of apoptosis marker CD95 and inflammatory marker cyclooxygenase-2 (COX-2 were carried out. In reflux esophagitis, most characteristic are changes in multilayered epithelium — hyperkeratosis, parakeratosis, proliferation of basal cells and acanthosis. At that, significant differences in morphometric characteristics of patients with normal BMI and overweight were not found. The development of reflux esophagitis was associated with violations of pro-apoptotic ability of esophageal mucosa cells and increased expression of COX-2, most significant in patients with high BMI. Detected disorders may determine the mechanisms of complicated course of GERD in these patients.

  13. VISUAL ASSESSMENT OF THE MUCOSA OF THE COLON IN IRRITABLE BOWEL SYNDROME IN THE DYNAMICS OF TREATMENT

    Directory of Open Access Journals (Sweden)

    A. E. Shklyaev

    2016-01-01

    Full Text Available Introduction. Irritable bowel syndrome (IBS is a common functional disorder of the gastrointestinal tract. The difficulty of its diagnosis related to the lack of specific markers of the disease. This article describes the visual changes of the mucosa of the colon in irritable bowel syndrome in the dynamics of medical and physiotherapy treatment. Materials and methods. Examined and treated 100 patients with endoscopically confirmed diagnosis of IBS. Of which 50 people were receiving pharmacotherapy and 50 –sinusoidal modulated currents — phoresis natural brine. Given the morphological evaluation of the mucosa of the intestine in the dynamics of treatment. Results. In IBS morpho-endoscopically detected dyskinetic disorders, minimal signs of nonspecific inflammation of the mucosa of the colon and primary degenerative manifestations. These changes are reversible and can be used to assess the quality of treatment. The high efficiency of SMC-phoresis natural brine in IBS.

  14. Alterações anatomopatologicas da mucosa gastrica associadas a infecção pelo helicobacter pylori

    OpenAIRE

    Correa, Esther Buzaglo Dantas

    1996-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciencias da Saude Foram comparadas as alterações anátomo-patológicas observadas na mucosa gástrica (inflamação, atividade da gastrite, atrofia, metaplasia intestinal e folículos linfóides) de 64 pacientes portadores de infecção pelo Helicobacter pylori com as alterações presentes na mucosa de 20 pacientes sem infecção. Inflamação, atividade da gastrite e folículos linfóides foram mais freqüentes na mucosa de corpo e...

  15. Function and importance of p63 in normal oral mucosa and squamous cell carcinoma of the head and neck

    DEFF Research Database (Denmark)

    Thurfjell, Niklas; Coates, Philip J; Boldrup, Linda

    2005-01-01

    BACKGROUND/AIMS: Squamous cell carcinoma of the head and neck (HNSCC) is the 6th most common malignancy worldwide with a 5-year survival that has not improved over the last 20-25 years. Factors of prognostic significance for this tumour type include the presence of regional lymph node metastasis...... and amplification of chromosome 3q21-29, where the p63 gene is located. This gene encodes 6 proteins and is crucial for formation of the oral mucosa, teeth, salivary glands and skin. Each of the 6 different p63 proteins has different characteristics and functions, where some resemble the tumour suppressor protein p......53, whilst others have functions that oppose p53. METHODS: To understand the function and importance of p63 in oral mucosa and tumour development we have studied protein as well as mRNA expression in normal oral mucosa and tumours. RESULTS/CONCLUSION: Expression of p63 proteins differs between...

  16. Iside effects of salicylates (ACK, corticosteroids and imunomodulators on the gastroduodenal mucosa - medication used in rheumatoid arthritis treatment

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    Popović M.

    2015-01-01

    Full Text Available The aim is to determine pathological -ulcero-erosive changes in the gastroduodenal mucosa (erosions, ulcers, ulcers, ulcer complications, subepithelial hemorrhage, etc. resulting from the adverse effects of salicylates /ACK/ corticosteroid, imunoodulatora which was prescribed in the treatment of rheumatoid arthritis (RA. The test was performed on 50 patients diagnosed with RA who were on the two-year continuous therapy. When respondents were done gastroscopy and pathological macroscopic changes resulting from the gastroduodenal mucosa as a result of adverse effects of administered drugs verified. After two years of preparations ACK controlled therapy, corticosteroids, imunomodula- sector on mucosa gastroduodenal erosions were found in 81.5% of patients, gastric ulcer in 33%, duodenal ulcer with 18.5% of patients and bleeding in 44.4%.

  17. Etiología del colesteatoma ótico

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    Julianis Loraine Quintero Noa

    2011-12-01

    Full Text Available El colesteatoma es una estructura quística caracterizada por la presencia de epitelio escamoso productor de queratina, que sustituye o recubre la mucosa normal en la hendidura del oído medio y ápex petroso, y puede ser causa de hipoacusia irreversible, destrucción ósea y graves complicaciones por su crecimiento expansivo. Clásicamente se describen los colesteatomas en congénitos y adquiridos. La etiología de su formación es multifactorial y continúa siendo poco claro y controversial. Se reportan diferentes teorías que han tratado de explicar el colesteatoma congénito, la transición de un bolsillo de retracción hasta la aparición del colesteatoma adquirido primario, y otras en la génesis del colesteatoma adquirido secundario. Se describe la presencia de algunas citoquinas dentro del colesteatoma que inducen la hiperproliferación e invasión incoordinada de los queratinocitos de la piel del conducto auditivo externo y la pars fláccida, más agresiva en el colesteatoma adquirido pediátrico, y que desempeñan un papel fundamental en la proliferación y en la apoptosis del queratinocito. En cultivo in vitro de una muestra de tejido colesteatomatoso, se ha identificado recientemente que el TNF-a estimula la producción de la IL-8. Se considera de interés ofrecer esta revisión sobre la etiología del colesteatoma, que aún se mantiene en el campo de la investigación y continúa siendo un reto para los otocirujanos por su alta incidencia de recidivas y posibles complicaciones.

  18. Comparação entre os bioenxertos de hidroxiapatita de cálcio e submucosa de intestino delgado porcino no preenchimento de defeitos ósseos criados em mandíbula de ratos Defect repair in rat mandible with hydroxyapatite cement comparad to small intestine submucosa

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    Andréa Thomaz Soccol

    2006-04-01

    Full Text Available OBJETIVO: O objetivo do presente estudo consiste em avaliar a regeneração óssea em defeito criado na mandíbula de ratos utilizando dois bioenxertos: hidroxiapatita de cálcio sintética e submucosa de intestino delgado porcina. FORMA DE ESTUDO: Experimental randomizado. MATERIAL E MÉTODO: Foram utilizados 24 ratos da linhagem Wisthar-Furth. Um defeito ósseo de 0,75cm x 1,5cm no corpo de cada hemimandíbula foi realizado em todos os animais com broca esférica de baixa rotação. Padronizou-se à esquerda o preenchimento do defeito ósseo, no grupo I com 15 microgramas de hidroxiapatita e no grupo II com preenchimento de submucosa de intestino delgado porcina (SID, e à direita o não-preenchimento serviu como controle. A eutanásia foi realizada no 40° dia de pós-operatório, após a qual se procederam as análises macroscópicas e histológicas das peças. RESULTADOS: O comprimento médio em milímetros das hemimandíbulas do grupo hidroxiapatita foi de 3,75, e o do grupo SID 3,03 e o do grupo controle foi de 2,63 (p: 0,0022. No grupo hidroxiapatita a neoformação óssea perfez uma área correspondente à 76,64% do total já no grupo SID 63,64% do total. CONCLUSÃO: Os resultados macroscópios e microscópicos foram superiores com a utilização do enxerto de hidroxiapatita quando comparado ao grupo submucosa de intestino delgado porcino. Entretanto os dois bioenxertos mostraram-se osteoindutores quando comparados ao controle.AIM: The aim of this study was to evaluate the bone formation in surgically created defects of rabbit mandibles by synthetic hydroxyapatite of calcium compared to small Intestine Submucosa. MATERIAL AND METHOD: 24 mices lineage Wisthar-Furth were used. A bony defect of 0,75 cm x 1,5 cm in mandibular ramus was accomplished in all animals. The hydroxyapatite implants were placed on the left hemimandiblein groupI, small Intestine submucosa in group II, and the right served as control. The euthanasia was accomplished

  19. Purification of a membrane-bound trypsin-like enzyme from the gut of the velvetbean caterpillar (Anticarsia gemmatalis Hübner =Purificação de uma enzima “tipo tripsina” não-solúvel do intestino da lagarta da soja (Anticarsia gemmatalis Hübner

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    Marcelo Matos Santoro

    2012-07-01

    Full Text Available Disruption of protein digestion in insects by specific endoprotease inhibitors is being regarded as an alternative to conventional insecticides for pest control. To optimize the effectiveness of this strategy, the understanding of the endoprotease diversity of the target insect is crucial. In this sense, a membrane-bound trypsin-like enzyme from the gut of Anticarsia gemmatalis fifth-instar larvae was purified. Non-soluble fraction of the gut extract was solubilized with 3-[(3-cholamidopropyldimethylammonio]-1-propanesulfonate (CHAPS and subjected to a p-aminobenzamidine affinity chromatography followed by anion-exchange chromatography. The yield of the purified enzyme was 11% with a purification factor of 143 and a final specific activity of 18.6 µM min.-1 mg-1 protein using N-α-benzoyl-L- Arg-p-nitroanilide (L-BApNA as substrate. The purified sample showed a single band with proteolytic activity active and apparent molecular mass of 25 kDa on SDS-PAGE. Molecular mass determined by MALDI-TOF mass spectrometry was 28,632 ± 26 Da. Although the low recovery and the difficulties in purifying large enzyme amounts limited its further characterization, the results contribute for the understanding of the proteases present on A. gemmatalis gut, which are potential targets for natural or specifically designed protease inhibitors.Comprometer a digestão de proteínas dos insetos pelo uso de inibidores específicos de endoproteases tem sido amplamente estudado como um método de controle de pragas alternativo ao uso dos inseticidas convencionais. No processo de otimização desta estratégia, o conhecimento da diversidade das endoproteases do inseto alvo torna-se crucial. Neste sentido, uma enzima “tipo-tripsina” ligada à membrana obtida do intestino de larvas do 5° instar de A. gemmatalis foi purificada. A fração insolúvel do extrato do intestino foi solubilizada com 3-[(3-cholamidopropyldimethylammonio]-1-propanesulfonate (CHAPS e submetida

  20. The rare presentations of a large polyp and an esophageal carcinoma in heterotropic gastric mucosa: a case series

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    Alagozlu Hakan

    2007-11-01

    Full Text Available Abstract Background Heterotopic gastric mucosa (HGM is commonly seen in the upper esophagus during endoscopyand is generally considered a benign disease. A hyperplastic polyp and an adenocarcinoma arising in heterotopic gastric mucosa are quite rare occurences. Case presentations We present two cases: The first is a patient who suffered from dysphagia because of a large hyperplastic polyp that arose from HGM; the polyp was excised endoscopically. Secondly, we report a rare case of adenocarcinoma arising in HGM of the cervical esophagus. Conclusion Morphologic changes or malignant transformation can develop in the inlet patch. Therefore, gastroenterologists should be aware of the possibility of HGM just distal to the upper esophageal sphincter.