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Sample records for mst rfp enhancement

  1. Ion Heating Anisotropy during Dynamo Activity in the MST RFP

    Science.gov (United States)

    den Hartog, D. J.; Chapman, J. T.; Craig, D.; Fiksel, G.; Fontana, P. W.

    1999-11-01

    MHD dynamo activity is large in the MST Reversed-Field Pinch during sawtooth crashes, and small otherwise. During a sawtooth crash, ion temperature increases rapidly to a level several times as high as the temperature between sawteeth, which itself can be larger than the electron temperature. Several theories have been developed to explain this ion heating, some indicating a possible asymmetry in perpendicular to parallel heating [C. G. Gimblett, Europhys. Lett. 11, 541 (1990); Z. Yoshida, Nucl. Fusion 31, 386 (1991); N. Mattor, P. W. Terry, and S. C. Prager, Comments Plasma Phys. Controlled Fusion 15, 65 (1992)]. In standard MST discharges, impurity ion temperature measured perpendicular to the magnetic field (T_⊥) is higher than impurity ion temperature parallel to the magnetic field (T_allel) during a sawtooth crash. Throughout the rest of the sawtooth cycle, T_⊥ EXTRAP-T2 RFP which showed T_⊥ < T_allel throughout the discharge [K. Sasaki et al., Plasma Phys. Control. Fusion 39, 333 (1997)

  2. Expanded Ion Energy Distribution Measurements on MST RFP Plasmas

    Science.gov (United States)

    Clark, Jerry; Titus, J. B.; Mezonlin, E. D.; Anderson, J. K.; Almagri, A. F.

    2016-10-01

    The Compact Neutral Particle Analyzer (CNPA) is a low energy (0.34 - 5.2 keV), high energy resolution (25 channels) neutral particle analyzer for ion energy distribution and temperature measurements on the Madison Symmetric Torus (MST). In MST plasmas during neutral beam injection, deuterium ions are known to have energies out to 40 keV. A retarding potential was built, installed, and calibrated to allow CNPA measurements to explore this region with high energy resolution, expanding ion energy distribution measurements, allowing us to better understand the dynamics of the bulk and fast ion populations during global magnetic reconnection events. Work supported by US DOE and NSF.

  3. Effect of Resonant Magnetic Perturbations on 3D equilibria in the MST RFP

    Science.gov (United States)

    Munaretto, Stefano

    2015-11-01

    The orientation of 3D, stellarator-like equilibria in the MST RFP can now be controlled with application of an m = 1 RMP. This has led to greatly improved diagnosis, revealing enhancements in both the central electron temperature and density. Coupled to a recent advance in the V3FIT code, reconstructions of the 3D equilibria have also been dramatically improved. The RMP also inhibits the generation of high-energy >20 keV electrons that is otherwise common with the 3D state. This state occurs when the normally broad spectrum of core-resonant m = 1 tearing modes condenses, with the innermost resonant mode growing to large amplitude, reaching ~ 8% of the axisymmetric field strength. This occurs in plasmas of sufficiently large Lundquist number ~ IpTe3/2, and the duration of the state is maximized with zero applied Bt (infinite toroidal beta). As the dominant mode grows, eddy current in MST's conducting shell slows the mode's rotation. This leads to locking of the 3D structure, but with an orientation that varies randomly shot to shot, making diagnosis difficult. An m = 1 RMP can now be applied with an array of saddle coils at the vertical insulated cut in the shell. With an amplitude br/B ~ 10% and a tailored temporal waveform, the RMP can force the 3D structure into any desired orientation relative to MST's diagnostics. A recent advance in V3FIT allows calculation of the substantial helical image current flowing in MST's shell, which has in turn allowed self-consistent utilization of both external and internal (Faraday rotation) measurements of the magnetic field. The ORBIT code predicts reduced stochasticity and improved confinement of high-energy electrons within the 3D structure. The suppression of these electrons by the m = 1 RMP may reflect a change to the central magnetic topology. The generation of these electrons is unaffected by non-resonant perturbations, such as m = 3. Supported by the US DOE.

  4. Measuring viscosity with a resonant magnetic perturbation in the MST RFP

    Science.gov (United States)

    Fridström, Richard; Munaretto, Stefano; Frassinetti, Lorenzo; Chapman, Brett; Brunsell, Per; Sarff, John; MST Team

    2016-10-01

    Application of an m = 1 resonant magnetic perturbation (RMP) causes braking and locking of naturally rotating m = 1 tearing modes (TMs) in the MST RFP. The experimental TM dynamics are replicated by a theoretical model including the interaction between the RMP and multiple TMs [Fridström PoP 23, 062504 (2016)]. The viscosity is the only free parameter in the model, and it is chosen such that model TM velocity evolution matches that of the experiment. The model does not depend on the means by which the natural rotation is generated. The chosen value of the viscosity, about 40 m2/s, is consistent with separate measurements in MST using a biased probe to temporarily spin up the plasma. This viscosity is about 100 times larger than the classical prediction, likely due to magnetic stochasticity in the core of these plasmas. Viscosity is a key parameter in visco-resistive MHD codes like NIMROD. The validation of these codes requires measurement of the viscosity over a broad parameter range, which will now be possible with the RMP technique that, unlike the biased probe, is not limited to low-energy-density plasmas. Estimation with the RMP technique of the viscosity in several MST discharges suggests that the viscosity decreases as the electron beta increases. Work supported by USDOE.

  5. Improved-confinement plasmas at high temperature and high beta in the MST RFP

    Energy Technology Data Exchange (ETDEWEB)

    Chapman, B. E. [University of Wisconsin, Madison; Ahn, J. W. [University of Wisconsin, Madison; Almagri, A. F. [University of Wisconsin, Madison; Anderson, J. [University of Wisconsin, Madison; Combs, Stephen Kirk [ORNL; Foust, Charles R [ORNL; Kaufman, M. [University of Wisconsin, Madison

    2009-01-01

    We have increased substantially the electron and ion temperatures, the electron density, and the total beta in plasmas with improved energy confinement in the Madison Symmetric Torus (MST). The improved confinement is achieved with a well-established current profile control technique for reduction of magnetic tearing and reconnection. A sustained ion temperature > 1 keV is achieved with intensified reconnection-based ion heating followed immediately by current profile control. In the same plasmas, the electron temperature reaches 2 keV, and the electron thermal diffusivity drops to about 2 m(2) s(-1). The global energy confinement time is 12 ms. This and the reported temperatures are the largest values yet achieved in the reversed-field pinch (RFP). These results were attained at a density similar to 10(19) m(-3). By combining pellet injection with current profile control, the density has been quadrupled, and total beta has nearly doubled to a record value of about 26%. The Mercier criterion is exceeded in the plasma core, and both pressure-driven interchange and pressure-driven tearing modes are calculated to be linearly unstable, yet energy confinement is still improved. Transient momentum injection with biased probes reveals that global momentum transport is reduced with current profile control. Magnetic reconnection events drive rapid momentum transport related to large Maxwell and Reynolds stresses. Ion heating during reconnection events occurs globally, locally, or not at all, depending on which tearing modes are involved in the reconnection. To potentially augment inductive current profile control, we are conducting initial tests of current drive with lower-hybrid and electron-Bernstein waves.

  6. Enhanced cytotoxic T-cell function and inhibition of tumor progression by Mst1 deficiency.

    Science.gov (United States)

    Yasuda, Kaneki; Ueda, Yoshihiro; Ozawa, Madoka; Matsuda, Tadashi; Kinashi, Tatsuo

    2016-01-01

    Mammalian ste-20 like kinase Mst1 plays important roles during apoptosis, proliferation, cell polarity, and migration. Here, we report a novel role of Mst1 for cytotoxic T-cell responses and tumor suppression. The defect of Mst1 caused decreased levels of FoxO, and promoted cytotoxicity in vitro. Mst1(-/-) cytotoxic T cells also exhibited enhanced T-bet expression that was associated with elevated expression levels of IFNγ and granzyme B. Moreover, Mst1(-/-) cytotoxic T cells suppressed tumor growth in vivo. The data suggest that Mst1 inhibits cytotoxicity via T-bet suppression by FoxO1 and FoxO3a. Thus, Mst1 is a potential therapeutic target for tumor immunotherapy.

  7. A new active array MST radar system with enhanced capabilities for high resolution atmospheric observations

    Science.gov (United States)

    Durga rao, Meka; Jayaraman, Achuthan; Patra, Amit; Venkat Ratnam, Madineni; Narayana Rao, T.; Kamaraj, Pandian; Jayaraj, Katta; Kmv, Prasad; Kamal Kumar, J.; Raghavendra, J.; Prasad, T. Rajendra; Thriveni, A.; Yasodha, Polisetti

    2016-07-01

    A new version of the 53-MHz MST Radar, using the 1024 solid state Transmit-Receive Modules (TRM), necessary feeder network, multi-channel receiver and a modified radar controller has been established using the existing antenna array of 1024 crossed Yagis. The new system has been configured for steering the beam on a pulse-to-pulse basis in all 360o azimuth and 20o zenith angle, providing enhanced capability to study the Mesosphere-Stratosphere-Troposphere and Ionosphere. The multi channel receiver system has been designed for Spaced Antenna (SA) and Interferometry/ Iamging applications. The new system has also been configured for radiating in circular polarization for its application in the Ionosphere Incoherent Scatter mode. The new active array MST radar at Very-High-Frequency (53-MHz) located at Gadanki (13.45°N, 79.18°E), a tropical station in India, will be used to enhance the observations of winds, turbulence during the passage of convective events over the radar site as deep convection occurs very often at tropical latitudes. The new configuration with enhanced average power, beam agility with multi-channel experiments will be a potential source for studying middle atmosphere and ionosphere. In this paper, we present the system configuration, new capabilities and the first results obtained using the new version of the MST Radar.

  8. Overview of MST Research

    Science.gov (United States)

    Sarff, J. S.

    2016-10-01

    MST progress in advancing the RFP for (1) fusion plasma confinement with ohmic heating and minimal external magnetization, (2) predictive capability in toroidal confinement physics, and (3) basic plasma physics is summarized. Validation of key plasma models is a program priority. Programmable power supplies (PPS) are being developed to maximize inductive capability. Well-controlled flattops with current as low as 0.02 MA are produced with an existing PPS, and Ip PPS under construction. The Lundquist number spans S =10(4 - 9) for 0.02-0.8 MA, allowing nonlinear MHD validation using NIMROD and DEBS at low S to be connected to highest S experiments. The PPS also enables MST tokamak operation for studying transients and runaway electron suppression with RMPs. Gyrokinetic modeling with GENE predicts unstable TEM in improved-confinement plasmas. Fluctuations are measured with TEM properties including a density-gradient threshold larger than for tokamak plasmas. Probe measurements hint that drift waves are also excited via the turbulent cascade in standard RFP plasmas. Turbulent energization of an electron tail occurs during sawtooth reconnection. New diagnostics are being developed to measure the energetic ion profile and transport from EP instabilities with NBI. Supported by US DoE and NSF.

  9. Plasma behaviour at high beta and high density in the Madison Symmetric Torus RFP

    Energy Technology Data Exchange (ETDEWEB)

    Wyman, M. [University of Wisconsin, Madison; Chapman, B. E. [University of Wisconsin, Madison; Ahn, J. W. [University of Wisconsin, Madison; Almagri, A. F. [University of Wisconsin, Madison; Anderson, J. [University of Wisconsin, Madison; Bonomo, F. [Consorzio RFX, Italy; Bower, D L [University of California, Los Angeles; Combs, Stephen Kirk [ORNL; Craig, D. [University of Wisconsin, Madison; Foust, Charles R [ORNL

    2009-01-01

    Pellet fuelling of improved confinement Madison Symmetric Torus (MST) plasmas has resulted in high density and high plasma beta. The density in improved confinement discharges has been increased fourfold, and a record plasma beta (beta(tot) = 26%) for the improved confinement reversed-field pinch (RFP) has been achieved. At higher beta, a new regime for instabilities is accessed in which local interchange and global tearing instabilities are calculated to be linearly unstable, but experimentally, no severe effect, e. g., a disruption, is observed. The tearing instability, normally driven by the current gradient, is driven by the pressure gradient in this case, and there are indications of increased energy transport ( as compared with low-density improved confinement). Pellet fuelling is also compared with enhanced edge fuelling of standard confinement RFP discharges for the purpose of searching for a density limit in MST. In standard-confinement discharges, pellet fuelling peaks the density profile where edge fuelling cannot, but transport appears unchanged. For a limited range of plasma current, MST discharges with edge fuelling are constrained to a maximum density corresponding to the Greenwald limit. This limit is surpassed in pellet-fuelled improved confinement discharges.

  10. Observation of Electron Bernstein Wave Heating in the MST Reversed Field Pinch

    Science.gov (United States)

    Seltzman, Andrew; Anderson, Jay; Dubois, Ami; Almagri, Abdulgader; Nonn, Paul; McCollam, Karsten; Chapman, Brett; Goetz, John; Forest, Cary

    2016-10-01

    We report the first observation of electron Bernstein wave heating in the MST RFP. Similar to a high density stellarator, the RFP is inaccessible to electromagnetic ECRH. The plasma current and |B|operating range of MST allows a 5.5 GHz RF source (100kW, 4ms pulse) to heat on the fundamental and up to 4th harmonic EC resonances. With an x-ray diagnostic most sensitive to edge electrons located +12 degrees toroidally from the antenna, the measured emission is a strong function of predicted heating inside versus outside the Bt =0 reversal layer of the RFP. Measured during a scan of plasma current, distinct edges in a plot of emissivity versus predicted deposition layer align with the deposition layers crossing of this reversal layer and confirm EBW heating on the fundamental through 4th EC harmonic. Additional confirmation of the absorption location has been demonstrated by using auxiliary poloidal current drive to reduce electron diffusion rates and sweep the location of the Bt =0 surface across a static RF absorption location in RFP discharges. In these discharges EBW enhancement of the 15-40keV x-ray energies has been observed. Work supported by USDOE.

  11. A collimated neutron detector for RFP plasmas in MST

    Science.gov (United States)

    Capecchi, W. J.; Anderson, J. K.; Bonofiglo, P. J.; Kim, J.; Sears, S.

    2016-11-01

    The neutron emissivity profile in the Madison Symmetric Torus is being reconstructed through the use of a collimated neutron detector. A scintillator-photomultiplier tube (PMT) system is employed to detect the fusion neutrons with the plasma viewing volume defined by a 55 cm deep, 5 cm diameter aperture. Effective detection of neutrons from the viewing volume is achieved through neutron moderation using 1300 lbs of high density polyethylene shielding, which modeling predicts attenuates the penetrating flux by a factor of 104 or more. A broad spectrum of gamma radiation is also present due to the unconfined fusion proton bombardment of the thick aluminum vacuum vessel. A 15 cm cylindrical liquid scintillator of 3.8 cm diameter is used to further increase directional sensitivity. A fast (5 ns rise time) preamplifier and digitization at 500 MHz prevent pulse pile-up even at high count rates (˜104/s). The entire neutron camera system is situated on an adjustable inclining base which provides the differing plasma viewing volumes necessary for reconstruction of the neutron emissivity profile. This profile, directly related to the fast-ion population, allows for an investigation of the critical fast-ion pressure gradient required to destabilize a neutral beam driven Alfvénic mode which has been shown to transport fast ions.

  12. Maximizing MST's inductive capability with a Bp programmable power supply

    Science.gov (United States)

    Chapman, B. E.; Holly, D. J.; Jacobson, C. M.; McCollam, K. J.; Morin, J. C.; Sarff, J. S.; Squitieri, A.

    2016-10-01

    A major goal of the MST program is the advancement of inductive control for the development of both the RFP's fusion potential and, synergistically, the predictive capability of fusion science. This entails programmable power supplies (PPS's) for the Bt and Bp circuits. A Bt PPS is already in place, allowing advanced RFP operation and the production of tokamak plasmas, and a Bp PPS prototype is under construction. To explore some of the new capabilities to be provided by the Bp PPS, the existing Bt PPS has been temporarily connected to the Bp circuit. One key result is new-found access to very low Ip (20 kA) and very low Lundquist number, S (104). At this low S, simulation of RFP plasmas with the MHD code NIMROD is readily achievable, and work toward validation of extended MHD models using NIMROD is underway with direct comparisons to these MST plasmas. The full Bp PPS will also provide higher Ip and S than presently possible, allowing MST to produce plasmas with S spanning as much as five orders of magnitude, a dramatic extension of MST's capability. In these initial tests, the PPS has also increased five-fold MST's Ip flattop duration, to about 100 ms. This, coupled with the recently demonstrated PPS ability to drive large-amplitude sinusoidal oscillations in Ip, will allow tests of extended-duration oscillating field current drive, the goal of which is ac sustainment of a quasi-dc plasma current. Work supported by US DOE.

  13. MST Filterability Tests

    Energy Technology Data Exchange (ETDEWEB)

    Poirier, M. R. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Burket, P. R. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Duignan, M. R. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL)

    2015-03-12

    The Savannah River Site (SRS) is currently treating radioactive liquid waste with the Actinide Removal Process (ARP) and the Modular Caustic Side Solvent Extraction Unit (MCU). The low filter flux through the ARP has limited the rate at which radioactive liquid waste can be treated. Recent filter flux has averaged approximately 5 gallons per minute (gpm). Salt Batch 6 has had a lower processing rate and required frequent filter cleaning. Savannah River Remediation (SRR) has a desire to understand the causes of the low filter flux and to increase ARP/MCU throughput. In addition, at the time the testing started, SRR was assessing the impact of replacing the 0.1 micron filter with a 0.5 micron filter. This report describes testing of MST filterability to investigate the impact of filter pore size and MST particle size on filter flux and testing of filter enhancers to attempt to increase filter flux. The authors constructed a laboratory-scale crossflow filter apparatus with two crossflow filters operating in parallel. One filter was a 0.1 micron Mott sintered SS filter and the other was a 0.5 micron Mott sintered SS filter. The authors also constructed a dead-end filtration apparatus to conduct screening tests with potential filter aids and body feeds, referred to as filter enhancers. The original baseline for ARP was 5.6 M sodium salt solution with a free hydroxide concentration of approximately 1.7 M.3 ARP has been operating with a sodium concentration of approximately 6.4 M and a free hydroxide concentration of approximately 2.5 M. SRNL conducted tests varying the concentration of sodium and free hydroxide to determine whether those changes had a significant effect on filter flux. The feed slurries for the MST filterability tests were composed of simple salts (NaOH, NaNO2, and NaNO3) and MST (0.2 – 4.8 g/L). The feed slurry for the filter enhancer tests contained simulated salt batch 6 supernate, MST, and filter enhancers.

  14. Electron Bernstein Wave Studies in MST

    Science.gov (United States)

    Seltzman, Andrew; Anderson, Jay; Forest, Cary; Nonn, Paul; Thomas, Mark; Almagri, Abdulgader; Chapman, Brett; Dubois, Ami; Goetz, John; McCollam, Karsten

    2015-11-01

    The RFP plasma is inaccessible to ECRH, requiring the electron Bernstein wave (EBW) for edge localized heating and current drive. MST is capable of generating RFPs or overdense tokamaks with Bt(0) ~ 0.08-0.14T in which a 5.55 GHz RF source (450kW, 2ms pulse) can heat at fundamental and harmonic EC resonances. The design of a suitable antenna is challenging in the RFP due to a magnetic field geometry that requires a low-field-side launch. The small vacuum gap between the close-fitting conducting shell and plasma leads to substantial antenna-plasma interaction. A minimized port hole size is required to limit error fields. Even so the port hole induced magnetic field perturbation in the antenna near-field that affects the mode conversion process and introduces EC resonances. A 5cm diameter cylindrical antenna centered in 5cm and 11cm diameter portholes is used. A multi-chord time-resolved x-ray detector and GENRAY ray tracing verifies EBW heating at higher harmonics in an MST tokamak with 10-40keV detected x-ray energies. Evidence of RF-induced emission from absorption at higher harmonics (4th / 5th) in low current RFP discharges has been observed. Simultaneous reflected power changes correspond to termination of x-ray emission indicating power limits. Work supported by USDOE.

  15. Global confinement in the MST (Madison Symmetric Torus) reversed field pinch

    Energy Technology Data Exchange (ETDEWEB)

    Almagri, A.; Assadi, S.; Beckstead, J.; Chartas, G.; Cudzinovic, M.; Den Hartog, D.; Dexter, R.; Hokin, S.; Holly, D.; Klevans, E.; Nebel, R.; Prager, S.; Rempel, T.; Sarff, J.; Scime, E.; Shen, W.; Spragins, C.; Sprott, C.; Starr, G.; Stoneking, M.; Veerasingam, R.; Watts, C.

    1990-10-01

    Global confinement measured in the first six months of MST full design operation is summarized. Central electron temperature and enhancement of resistivity over the Z = 1 Spitzer value are similar to other RFP experiments for the same value of I/N. As in several other RFP experiments, energy confinement time and poloidal beta are found to decrease with increasing plasma current, with maximum values of {tau}{sub E} {approximately} 1 ms and {beta}{sub p} {approximately} 10%. Particle transport may be approximated with a diffusion coefficient D {approximately} 40 m{sup 2}/s for a discharge studied with a 1-d particle-neutral code. A more elaborate code, incorporating heat and impurity transport, indicates an increase of Z{sub eff} with I/N. This code also reveals that the toroidal magnetic field decays resistively between discrete dynamo events, at the rate given by the measured global resistivity. Edge suprathermal electrons are observed as on other RFPs, with temperatures comparable to the central electron temperature and carrying a current density at least 15% of the total measured with an insertable magnetic coil array. Radial magnetic profiles measured with this array may be matched with a Modified Polynomial Function Model equilibrium for a value of {beta}{sub p} which is a factor of two larger than measured, but which is required due to the large values of {Theta} for a given F found in MST discharges ({Theta} = 1.85 at F= {minus}0.15). 15 refs., 14 figs.

  16. Overview of MST results and plans

    Science.gov (United States)

    Sarff, J. S.

    2010-11-01

    Advances in RFP performance and basic physics studies on MST are summarized. Pellet injection increases the density of improved confinement plasmas well above the empirical limit (up to n/nG=1.5), and the beta value is likewise increased, without evidence for a hard limit. A 1 MW tangential neutral beam injector is operational, sparking several new topics for RFP research. The observed good confinement of fast ions bodes well for increasing beta and assessing energetic ion effects. The NBI's directed momentum affects the plasma flow, and altered tearing mode dynamics suggest localized current drive in the core. Energy confinement with oscillating field current drive is measured about the same as for steady induction, a promising result for this efficient form of current sustainment. Magnetic self-organization studies reveal that ion heating during magnetic reconnection events is increasingly anisotropic at lower density. Detailed measurements of the flow and magnetic structure of the reconnection region around the q=0 surface emphasize the importance of nonlinear mode coupling and two-fluid physics. Several new diagnostics are being implemented, including fast Thomson scattering, neutral particle analyzers, and fast ion CHERS. Continuing investigation of lower hybrid and electron Bernstein waves will be summarized. Work supported by USDoE and NSF.

  17. MST Progress and Plans

    Science.gov (United States)

    Sarff, J. S.

    2002-11-01

    New diagnostic capability combined with turbulence control is producing a clearer picture of magnetic fluctuation-induced transport. Local stochastic transport depends on both the mode amplitudes and the density of nearby resonant surfaces. Radially resolved measurements of the electron heat diffusivity agree with theoretical estimates ˜ v_th(b/B)^2, but only where the resonant surface density is high. With auxiliary current drive, broadband mode suppression reduces the local transport to create RFP global confinement comparable to tokamak plasmas. In this case an increased population of high energy electrons (inferred from hard x-ray emission) is inconsistent with the velocity dependence characteristic of stochastic diffusion. This plus direct measurement of reduced fluctuations in the core by FIR polarimetry evidence closed magnetic surface formation. Core electrostatic fluctuations are also measured with a heavy ion beam probe for the first time in an RFP. The nonlinear evolution of the magnetic spectrum is studied using bi-spectral techniques, and, for the first time, m=0 modes are measured to be linearly stable (in standard RFP plasmas). An oscillating BT, applied to test ac helicity injection, entrains sawtooth magnetic relaxation processes. Ion temperature profiles are obtained using CHERS and Rutherford scattering. For future plasma control, a 2nd generation lower hybrid antenna shows improved performance, and electron Bernstein wave coupling is high in improved confinement plasmas, self-consistent with profile control requirements. Also, a new pellet injector provides core fueling. Work supported by US DOE.

  18. Mst1 inhibits CMECs autophagy and participates in the development of diabetic coronary microvascular dysfunction

    Science.gov (United States)

    Lin, Jie; Zhang, Lei; Zhang, Mingming; Hu, Jianqiang; Wang, Tingting; Duan, Yu; Man, Wanrong; Wu, Bin; Feng, Jiaxu; Sun, Lei; Li, Congye; Zhang, Rongqing; Wang, Haichang; Sun, Dongdong

    2016-01-01

    Cardiovascular complications account for a substantial proportion of morbidity and mortality in diabetic patients. Abnormalities of cardiac microvascular endothelial cells (CMECs) lead to impaired cardiac microvascular vessel integrity and subsequent cardiac dysfunction, underlining the importance of coronary microvascular dysfunction. In this study, experimental diabetes models were constructed using Mst1 transgenic, Mst1 knockout and sirt1 knockout mice. Diabetic Mst1 transgenic mice exhibited impaired cardiac microvessel integrity and decreased cardiac function. Mst1 overexpression deceased CMECs autophagy as evidenced by decreased LC3 expression and enhanced protein aggregation when subjected to high glucose culture. Mst1 knockout improved cardiac microvessel integrity and enhanced cardiac functions in diabetic mice. Mst1 knockdown up-regulated autophagy as indicated by more typical autophagosomes and increased LC3 expression in CMECs subjected to high glucose cultures. Mst1 knockdown also promoted autophagic flux in the presence of bafilomycin A1. Mst1 overexpression increased CMECs apoptosis, whereas Mst1 knockout decreased CMECs apoptosis. Sirt1 knockout abolished the effects of Mst1 overexpression in cardiac microvascular injury and cardiac dysfunction. In conclusion, Mst1 knockout preserved cardiac microvessel integrity and improved cardiac functions in diabetic mice. Mst1 decreased sirt1 activity, inhibited autophagy and enhanced apoptosis in CMECs, thus participating in the pathogenesis of diabetic coronary microvascular dysfunction. PMID:27680548

  19. Saccade-induced image motion cannot account for post-saccadic enhancement of visual processing in primate MST

    Directory of Open Access Journals (Sweden)

    Shaun L Cloherty

    2015-09-01

    Full Text Available Primates use saccadic eye movements to make gaze changes. In many visual areas, including the dorsal medial superior temporal area (MSTd of macaques, neural responses to visual stimuli are reduced during saccades but enhanced afterwards. How does this enhancement arise – from an internal mechanism associated with saccade generation or through visual mechanisms activated by the saccade sweeping the image of the visual scene across the retina? Spontaneous activity in MSTd is elevated even after saccades made in darkness, suggesting a central mechanism for post-saccadic enhancement. However, based on the timing of this effect, it may arise from a different mechanism than occurs in normal vision. Like neural responses in MSTd, initial ocular following eye speed is enhanced after saccades, with evidence suggesting both internal and visually mediated mechanisms. Here we recorded from visual neurons in MSTd and measured responses to motion stimuli presented soon after saccades and soon after simulated saccades – saccade-like displacements of the background image during fixation. We found that neural responses in MSTd were enhanced when preceded by real saccades but not when preceded by simulated saccades. Furthermore, we also observed enhancement following real saccades made across a blank screen that generated no motion signal within the recorded neurons’ receptive fields. We conclude that in MSTd the mechanism leading to post-saccadic enhancement has internal origins.

  20. 3-D Measurement of Recycling and Radiation in MST

    Science.gov (United States)

    Norval, Ryan; Goetz, John; Schmitz, Oliver

    2016-10-01

    The MST reversed-field pinch (RFP) can undergo spontaneous transition to a helical core state, associated with the growth of the innermost resonant magnetic mode. Currently multiple 2-D imaging cameras are in place allowing for nearly full vessel viewing and measurement of recycling and impurities fluxes. The transition from the standard to helical RFP causes an observable change in edge plasma. While in the helical state the plasma wall interaction (PWI) on MSTs poloidal limiter strongly correlates with the helicity of the core mode. PWI on the toroidal limiter overall is reduced, with the remaining PWI sites corresponding the helicity of the core mode, or the locations of diagnostic limiters and the error fields they create. EIRENE, a neutral particle code use for modeling edge plasmas, is used to compute the neutral profiles based on measured recycling fluxes. EIRENE computes the radiative and charge exchange power losses. Comparison is made between the standard and helical RFP plasmas. Bolometer measurements of total radiation are currently in progress to supplement the modeling. This work is supported by the U.S. Department of Energy.

  1. RESULTS OF SUPPLEMENTAL MST STUDIES

    Energy Technology Data Exchange (ETDEWEB)

    Peters, T; David Hobbs, D; Samuel Fink, S

    2006-07-24

    The current design of the Salt Waste Processing Facility (SWPF) includes an auxiliary facility, the Actinide Finishing Facility, which provides a second contact of monosodium titanate (MST) to remove soluble actinides and strontium from waste if needed. This treatment will occur after cesium removal by Caustic-Side Solvent Extraction (CSSX). Although the process changes and safety basis implications have not yet been analyzed, provisions also exist to recover the MST from this operation and return to the initial actinide removal step in the SWPF for an additional (third) contact with fresh waste. A U.S. Department of Energy (DOE) request identified the need to study the following issues involving this application of MST: Determine the effect of organics from the solvent extraction (CSSX) process on radionuclide sorption by MST; Determine the efficiency of re-using MST for multiple contacts; and Examine fissile loading on MST under conditions using a waste containing significantly elevated concentrations of plutonium, uranium, neptunium, and strontium. This report describes the results of three experimental studies conducted to address these needs: (1) Addition of high concentrations of entrained CSSX solvent had no noticeable effect, over a two week period, on the sorption of the actinides and strontium by MST in a direct comparison experiment. (2) Test results show that MST still retains appreciable capacity after being used once. For instance, reused MST--in the presence of entrained solvent--continued to sorb actinides and strontium. (3) A single batch of MST was used to sequentially contact five volumes of a simulant solution containing elevated concentrations of the radionuclides of interest. After the five contacts, we measured the following solution actinide loadings on the MST: plutonium: 0.884 {+-} 0.00539 wt % or (1.02 {+-} 0.0112) E+04 {micro}g/g MST, uranium: 12.1 {+-} 0.786 wt % or (1.40 {+-} 0.104) E+05 {micro}g/g MST, and neptunium: 0.426 {+-} 0

  2. Redemystifying MST1/hippo signaling

    Institute of Scientific and Technical Information of China (English)

    Lei Xiao; Zengqiang Yuan

    2010-01-01

    @@ Since the Mammalian Ste20-like kinases(MST)1/2 were first indentified in 1995,our knowledge about MST1/2 and its Drosophila ortholog Hippo has expanded to diverse biologic process ranging from cell survival and death,organ size control,to proliferation and tumorigenesis.

  3. An integrated data analysis tool for improving measurements on the MST RFP

    Energy Technology Data Exchange (ETDEWEB)

    Reusch, L. M., E-mail: lmmcguire@wisc.edu; Galante, M. E.; Johnson, J. R.; McGarry, M. B.; Den Hartog, D. J. [Physics Department, University of Wisconsin-Madison, Madison, Wisconsin 53706 (United States); Franz, P. [Consorzio RFX, EURATOM-ENEA Association, Padova (Italy); Stephens, H. D. [Physics Department, University of Wisconsin-Madison, Madison, Wisconsin 53706 (United States); Pierce College Fort Steilacoom, Lakewood, Washington 98498 (United States)

    2014-11-15

    Many plasma diagnostics contain complementary information. For example, the double-foil soft x-ray system (SXR) and the Thomson Scattering diagnostic (TS) on the Madison Symmetric Torus both measure electron temperature. The complementary information from these diagnostics can be combined using a systematic method based on integrated data analysis techniques, leading to more accurate and sensitive results. An integrated data analysis tool based on Bayesian probability theory was able to estimate electron temperatures that are consistent with both the SXR and TS diagnostics and more precise than either. A Markov Chain Monte Carlo analysis to increase the flexibility of the tool was implemented and benchmarked against a grid search method.

  4. Charge-to-mass-ratio-dependent ion heating during magnetic reconnection in the MST RFP

    Energy Technology Data Exchange (ETDEWEB)

    Kumar, S. T. A.; Almagri, A. F.; Den Hartog, D. J.; Nornberg, M. D.; Sarff, J. S.; Terry, P. W. [Department of Physics, University of Wisconsin-Madison, Madison, Wisconsin 53706 (United States); Center for Magnetic Self-Organization in Laboratory and Astrophysical Plasmas, University of Wisconsin-Madison, Madison, Wisconsin 53706 (United States); Craig, D. [Wheaton College, Wheaton, Illinois 60187 (United States)

    2013-05-15

    Temperature evolution during magnetic reconnection has been spectroscopically measured for various ion species in a toroidal magnetized plasma. Measurements are made predominantly in the direction parallel to the equilibrium magnetic field. It is found that the increase in parallel ion temperature during magnetic reconnection events increases with the charge-to-mass ratio of the ion species. This trend can be understood if the heating mechanism is anisotropic, favoring heating in the perpendicular degree of freedom, with collisional relaxation of multiple ion species. The charge-to-mass ratio trend for the parallel temperature derives from collisional isotropization. This result emphasizes that collisional isotropization and energy transfer must be carefully modeled when analyzing ion heating measurements and comparing to theoretical predictions.

  5. Classical confinement and outward convection of impurity ions in the MST RFP

    Energy Technology Data Exchange (ETDEWEB)

    Kumar, S. T. A.; Den Hartog, D. J.; Mirnov, V. V.; Eilerman, S.; Nornberg, M.; Reusch, J. A.; Sarff, J. S. [Department of Physics, University of Wisconsin-Madison, Madison, Wisconsin 53706 (United States); Center for Magnetic Self-Organization in Laboratory and Astrophysical Plasmas, University of Wisconsin-Madison, Madison, Wisconsin 53706 (United States); Caspary, K. J.; Chapman, B. E.; Parke, E. [Department of Physics, University of Wisconsin-Madison, Madison, Wisconsin 53706 (United States); Magee, R. M. [Department of Physics, West Virginia University, Morgantown, WV 26506 (United States); Brower, D. L.; Ding, W. X.; Lin, L. [Department of Physics and Astronomy, University of California, Los Angeles, California 90095 (United States); Craig, D. [Physics Department, Wheaton College, Wheaton, Illinois 60187 (United States); Fiksel, G. [Center for Magnetic Self-Organization in Laboratory and Astrophysical Plasmas, University of Wisconsin-Madison, Madison, Wisconsin 53706 (United States); Laboratory for Laser Energetics, University of Rochester, Rochester, New York (United States)

    2012-05-15

    Impurity ion dynamics measured with simultaneously high spatial and temporal resolution reveal classical ion transport in the reversed-field pinch. The boron, carbon, oxygen, and aluminum impurity ion density profiles are obtained in the Madison Symmetric Torus [R. N. Dexter et al., Fusion Technol. 19, 131 (1991)] using a fast, active charge-exchange-recombination-spectroscopy diagnostic. Measurements are made during improved-confinement plasmas obtained using inductive control of tearing instability to mitigate stochastic transport. At the onset of the transition to improved confinement, the impurity ion density profile becomes hollow, with a slow decay in the core region concurrent with an increase in the outer region, implying an outward convection of impurities. Impurity transport from Coulomb collisions in the reversed-field pinch is classical for all collisionality regimes, and analysis shows that the observed hollow profile and outward convection can be explained by the classical temperature screening mechanism. The profile agrees well with classical expectations. Experiments performed with impurity pellet injection provide further evidence for classical impurity ion confinement.

  6. Effects of pulsed electric field on ULQ and RFP plasmas

    Energy Technology Data Exchange (ETDEWEB)

    Watanabe, M. [Iwate Univ., Morioka (Japan). Faculty of Engineering; Saito, K.; Suzuki, T. [and others

    1997-12-31

    Dynamo activity and self-organization processes are investigated using the application of pulsed poloidal and toroidal electric fields on ULQ and RFP plasmas. Synchronized to the application of the pulsed electric fields, the remarkable responses of the several plasma parameters are observed. The plasma has a preferential magnetic field structure, and the external perturbation activates fluctuation to maintain the structure through dynamo effect. This process changes the total dissipation with the variation of magnetic helicity in the system, showing that self organization accompanies an enhanced dissipation. (author)

  7. Interdigital Line Antennas for Launching LH Waves in MST

    Science.gov (United States)

    Thomas, M. A.; Goetz, J. A.; Oliva, S. P.

    2002-11-01

    RF current drive has been proposed as a method for reducing the tearing fluctuations that are responsible for anomalous energy transport in the RFP. A system for launching lower hybrid slow waves at 800 MHz and n_||= 7.5 is now in operation on MST. The antenna is an enclosed interdigital line using λ/4 resonators with an opening in the cavity through which the wave is coupled to the plasma. A new antenna has been built incorporating several design improvements. These include larger vacuum feedthroughs, better impedance matching, internal instrumentation and improved directionality. Power handling is 3-4 times that of the original and continues to improve with conditioning. Further design improvements are underway to optimize impedance matching and damping rate along the traveling wave structure.

  8. MST kinases in development and disease

    OpenAIRE

    Thompson, Barry J.; Sahai, Erik

    2015-01-01

    The mammalian MST kinase family, which is related to the Hippo kinase in Drosophila melanogaster, includes five related proteins: MST1 (also called STK4), MST2 (also called STK3), MST3 (also called STK24), MST4, and YSK1 (also called STK25 or SOK1). MST kinases are emerging as key signaling molecules that influence cell proliferation, organ size, cell migration, and cell polarity. Here we review the regulation and function of these kinases in normal physiology and pathologies, including cance...

  9. MST kinases in development and disease.

    Science.gov (United States)

    Thompson, Barry J; Sahai, Erik

    2015-09-14

    The mammalian MST kinase family, which is related to the Hippo kinase in Drosophila melanogaster, includes five related proteins: MST1 (also called STK4), MST2 (also called STK3), MST3 (also called STK24), MST4, and YSK1 (also called STK25 or SOK1). MST kinases are emerging as key signaling molecules that influence cell proliferation, organ size, cell migration, and cell polarity. Here we review the regulation and function of these kinases in normal physiology and pathologies, including cancer, endothelial malformations, and autoimmune disease.

  10. RADIUM AND THORIUM SORPTION BY MONOSODIUM TITANATE (MST) AND MODIFIED MST (mMST)

    Energy Technology Data Exchange (ETDEWEB)

    Taylor-Pashow, K.; Hobbs, D.

    2012-02-15

    A series of tests were planned to examine the removal of Ra and Th by monosodium titanate (MST) and modified monosodium titanate (mMST). Simulated waste solutions were prepared containing Ra and Th, along with Sr, Np, Pu, and U. Following simulant preparation the simulants were filtered through 0.45-m filters. Analysis of the simulants indicated no Th in the filtered solution. This is due to the very low solubility of Th in alkaline solutions. Based on the reported detection limits for {sup 228}Th by gamma analyses, the solubility of Th in the simulant solutions is < 3.0E-10 g/L or < 1.3E-12 M. Therefore, data could not be obtained regarding the removal of Th by MST and mMST; however, testing proceeded to examine the removal of Ra. Sorption testing indicated that Ra, like Sr, is very rapidly removed from solution by both MST and mMST. The Ra concentration in solution fell below the method detection limit (MDL) within 30 minutes of contact with MST, and within 2 hours of contact with mMST, when tested at 25 C using a 5.6 M Na simulant. Additional testing examined the effects of ionic strength and temperature on the MST and mMST performance. Results from these tests showed that the majority of samples still reached a Ra concentration below the MDL, indicating excellent removal. For the highest ionic strength solution (6.6 M Na), there did appear to be a slight decrease in the Ra removal by mMST, as indicated by a larger number of samples just above the MDL. The effect of temperature on {sup 226}Ra removal is indeterminate for either MST or mMST in the temperature range (25-60 C) and concentrations studied since the final soluble concentration of Ra remained at or below the detection limits for all tests. Desorption testing was also performed using decontaminated salt solution (DSS) diluted to sodium concentrations of 2 M and 0.5 M, to represent the intermediate and final stages of washing. Results from these tests indicated no desorption of any sorbents, with the

  11. RfpA, RfpB, and RfpC are the Master Control Elements of Far-Red Light Photoacclimation (FaRLiP).

    Science.gov (United States)

    Zhao, Chi; Gan, Fei; Shen, Gaozhong; Bryant, Donald A

    2015-01-01

    Terrestrial cyanobacteria often occur in niches that are strongly enriched in far-red light (FRL; λ > 700 nm). Some cyanobacteria exhibit a complex and extensive photoacclimation response, known as FRL photoacclimation (FaRLiP). During the FaRLiP response, specialized paralogous proteins replace 17 core subunits of the three major photosynthetic complexes: Photosystem (PS) I, PS II, and the phycobilisome. Additionally, the cells synthesize both chlorophyll (Chl) f and Chl d. Using biparental mating from Escherichia coli, we constructed null mutants of three genes, rfpA, rfpB, and rfpC, in the cyanobacteria Chlorogloeopsis fritschii PCC 9212 and Chroococcidiopsis thermalis PCC 7203. The resulting mutants were no longer able to modify their photosynthetic apparatus to absorb FRL, were no longer able to synthesize Chl f, inappropriately synthesized Chl d in white light, and were unable to transcribe genes of the FaRLiP gene cluster. We conclude that RfpA, RfpB, and RfpC constitute a FRL-activated signal transduction cascade that is the master control switch for the FaRLiP response. FRL is proposed to activate (or inactivate) the histidine kinase activity of RfpA, which leads to formation of the active state of RfpB, the key response regulator and transcription activator. RfpC may act as a phosphate shuttle between RfpA and RfpB. Our results show that reverse genetics via conjugation will be a powerful approach in detailed studies of the FaRLiP response.

  12. Density-Gradient-Driven trapped-electron-modes in improved-confinement RFP plasmas

    Science.gov (United States)

    Duff, James

    2016-10-01

    Short wavelength density fluctuations in improved-confinement MST plasmas exhibit multiple features characteristic of the trapped-electron-mode (TEM), strong evidence that drift wave turbulence emerges in RFP plasmas when transport associated with MHD tearing is reduced. Core transport in the RFP is normally governed by magnetic stochasticity stemming from long wavelength tearing modes that arise from current profile peaking. Using inductive control, the tearing modes are reduced and global confinement is increased to values expected for a comparable tokamak plasma. The improved confinement is associated with a large increase in the pressure gradient that can destabilize drift waves. The measured density fluctuations have frequencies >50 kHz, wavenumbers k_phi*rho_sglobal tearing modes. Their amplitude increases with the local density gradient, and they exhibit a density-gradient threshold at R/L_n 15, higher than in tokamak plasmas by R/a. the GENE code, modified for RFP equilibria, predicts the onset of microinstability for these strong-gradient plasma conditions. The density-gradient-driven TEM is the dominant instability in the region where the measured density fluctuations are largest, and the experimental threshold-gradient is close to the predicted critical gradient for linear stability. While nonlinear analysis shows a large Dimits shift associated with predicted strong zonal flows, the inclusion of residual magnetic fluctuations causes a collapse of the zonal flows and an increase in the predicted transport to a level close to the experimentally measured heat flux. Similar circumstances could occur in the edge region of tokamak plasmas when resonant magnetic perturbations are applied for the control of ELMs. Work supported by US DOE.

  13. Gyrokinetic Studies of Microinstabilities in the RFP

    CERN Document Server

    Carmody, Daniel; Terry, P W

    2013-01-01

    An analytic equilibrium, the Toroidal Bessel Function Model, is used in conjunction with the gyrokinetic code GYRO to investigate the nature of microinstabilities in a reversed field pinch (RFP) plasma. The effect of the normalized electron plasma pressure ({\\beta}) on the characteristics of the microinstabilities is studied. A transition between an ion temperature gradient (ITG) driven mode and a microtearing mode as the dominant instability is found to occur at a {\\beta} value of approximately 4.5%. Suppression of the ITG mode occurs as in the tokamak, through coupling to shear Alfven waves, with a critical {\\beta} for stability higher than its tokamak equivalent due to a shorter parallel connection length. There is a steep dependence of the microtearing growth rate on temperature gradient suggesting high profile stiffness. There is evidence for a collisionless microtearing mode. The properties of this mode are investigated, and it is found that curvature drift plays an important role in the instability.

  14. Electron Bernstein Wave Emission from RFP Plasmas

    Science.gov (United States)

    Nornberg, M. D.; Thomas, M.; Anderson, J.; Forest, C. B.

    1998-11-01

    Electron cyclotron emission (ECE) has proven to be a powerfull diagnostic tool in tokamak plasmas for determining the time evolution of the electron temperature profile. The standard technique of observing O-mode or X-mode electromagnetic waves normal to the magnetic field is not applicable to reversed field pinch (RFP) plasmas since the plasma frequency is much larger than the electron cyclotron frequency. We are investigating the use of electron Bernstein waves (presumed to be in thermal equilibrium with the electrons) through the aip.org/journal_cgi/ getpdf?KEY=PRLTAO&cvips=PRLTAO000078000018003467000001>O-X-B mode conversion process. At oblique incidence, the evanescent layer separating the plamsa cutoff from the cyclotron cutoff vanishes, allowing conversion of the Bernstein mode waves to the extraordinary mode and finally to the ordinary mode. The O-mode radiation is received by a phased array antenna consisting of two waveguides on the edge of the plasma, and the spectrum of emitted radiation is measured using a radiometer spanning 4-8 GHz. In addition to providing information about the electron temperature profile, the spectrum can provide a novel method of measuring the central magnetic field strength for current profile reconstructions.

  15. RFP tags for labeling secretory pathway proteins

    Energy Technology Data Exchange (ETDEWEB)

    Han, Liyang; Zhao, Yanhua [State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082 (China); Zhang, Xi; Peng, Jianxin [College of Life Sciences, Central China Normal University, Wuhan 430079, Hubei (China); Xu, Pingyong, E-mail: pyxu@ibp.ac.cn [Key Laboratory of Interdisciplinary Research, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101 (China); Huan, Shuangyan, E-mail: shuangyanhuan@163.com [State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082 (China); Zhang, Mingshu, E-mail: mingshu1984@gmail.com [Key Laboratory of Interdisciplinary Research, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101 (China)

    2014-05-09

    Highlights: • Membrane protein Orai1 can be used to report the fusion properties of RFPs. • Artificial puncta are affected by dissociation constant as well as pKa of RFPs. • Among tested RFPs mOrange2 is the best choice for secretory protein labeling. - Abstract: Red fluorescent proteins (RFPs) are useful tools for live cell and multi-color imaging in biological studies. However, when labeling proteins in secretory pathway, many RFPs are prone to form artificial puncta, which may severely impede their further uses. Here we report a fast and easy method to evaluate RFPs fusion properties by attaching RFPs to an environment sensitive membrane protein Orai1. In addition, we revealed that intracellular artificial puncta are actually colocalized with lysosome, thus besides monomeric properties, pKa value of RFPs is also a key factor for forming intracellular artificial puncta. In summary, our current study provides a useful guide for choosing appropriate RFP for labeling secretory membrane proteins. Among RFPs tested, mOrange2 is highly recommended based on excellent monomeric property, appropriate pKa and high brightness.

  16. RFP for the Auroral Multiscale Midex (AMM) Mission star tracker

    DEFF Research Database (Denmark)

    Riis, Troels; Betto, Maurizio; Jørgensen, John Leif;

    1999-01-01

    This document is in response to the John Hopkins University - Applied Physics Laboratory RFP for the Auroral Multiscale Midex Mission star tracker.It describes the functionality, the requirements and the performance of the ASC Star Tracker.......This document is in response to the John Hopkins University - Applied Physics Laboratory RFP for the Auroral Multiscale Midex Mission star tracker.It describes the functionality, the requirements and the performance of the ASC Star Tracker....

  17. DEVELOPMENT OF MONOSODIUM TITANATE (MST) PURCHASE SPECIFICATIONS

    Energy Technology Data Exchange (ETDEWEB)

    Hobbs, D

    2006-04-30

    Savannah River National Laboratory (SRNL) evaluated the previous monosodium titanate (MST) purchase specifications for particle size and strontium decontamination factor. Based on the measured particle size and filtration performance characteristics of several MST samples with simulated waste solutions and various filter membranes we recommend changing the particle size specification as follows. The recommended specification varies with the size and manufacturer of the filter membrane as shown below. We recommend that future batches of MST received at SRS be tested for particle size and filtration performance. This will increase the available database and provide increased confidence that particle size parameters are an accurate prediction of filtration performance. Testing demonstrated the feasibility of a non-radiochemical method for evaluating strontium removal performance of MST samples. Using this analytical methodology we recommend that the purchase specification include the requirement that the MST exhibits a strontium DF factor of >1.79 upon contact with a simulated waste solution with composition as reported for simulated waste solution SWS-7-2005-1 in Table 1 and containing 5.2 to 5.7 mg L{sup -1} strontium with 0.1 g L{sup -1} of the MST. We also recommend performing additional tests with these simulants and MST samples and, if available, new MST samples, to determine the reproducibility and increase the available database for the measurements by the ICP-ES instrument. These measurements will provide increased confidence that the non-radiological method provides a reliable method for evaluating the strontium and actinide removal performance for MST samples.

  18. Effects of Resistivity and Viscosity on m =0 Rise and Fall Time in the RFP

    Science.gov (United States)

    Futch, A. M.; Craig, D.; Hesse, R.; Jacobson, C. M.

    2016-10-01

    In the reversed field pinch (RFP), poloidal mode number m =0 fluctuations are driven in a sawtooth cycle via nonlinear coupling with unstable m =1 tearing modes. We explore how the rise and fall time of these m =0 fluctuations depends on resistivity and viscosity in visco-resistive MHD simulations using the DEBS code. Both the Lundquist number (S) and magnetic Prandtl number (Pr) affect the rise/fall time. Analysis of MST experimental data also shows that both the rise and fall times of the m =0 amplitude vary with S. The variation observed in experiment is consistent with simulation results for rise time, but shows some differences for fall time. Rise time is insensitive to the resistivity profile but depends slightly on the viscosity profile. Fall time is strongly correlated with the duration of the crash which depends on both resistivity and viscosity profiles. These results suggest that the rise and fall time of the m =0 modes at the sawtooth crash is not strongly influenced by the local resistivity near the resonant surface but instead is primarily determined by the overall dynamics of the entire sawtooth cycle. The role of viscosity is less clear though the edge viscosity affects the m =0 evolution more than the core. This work has been supported by the U.S.D.O.E.

  19. MST radar data-base management

    Science.gov (United States)

    Wickwar, V. B.

    1983-01-01

    Data management for Mesospheric-Stratospheric-Tropospheric, (MST) radars is addressed. An incoherent-scatter radar data base is discussed in terms of purpose, centralization, scope, and nature of the data base management system.

  20. RFX machine and power supply improvements for RFP advanced studies

    Energy Technology Data Exchange (ETDEWEB)

    Piovan, R. E-mail: piovan@igi.pd.cnr.it; Gnesotto, F.; Ortolani, S.; Baker, W.; Barana, O.; Bettini, P.; Cavazzana, R.; Chitarin, G.; Dal Bello, S.; De Lorenzi, A.; Fiorentin, P.; Gaio, E.; Grando, L.; Luchetta, A.; Manduchi, G.; Marchiori, G.; Marcuzzi, D.; Masiello, A.; Milani, F.; Peruzzo, S.; Pomaro, N.; Sonato, P.; Taliercio, C.; Toigo, V.; Zaccaria, P.; Zanotto, L.; Zollino, G

    2001-10-01

    Experimental results and theoretical studies call for Reversed Field Experiment (RFX) machine and power supply improvements to allow studies that go beyond those of a conventional Reversed Field Pinch (RFP) with passively stabilized turbulent MHD dynamo. The new paths opened by recent results in RFX and other RFP machines are introduced; then the goals and the design lines of the technical modifications of RFX, mainly addressed to improve the first wall, the plasma magnetic boundaries and to increase the operational flexibility of the toroidal field circuit power supply, are reported.

  1. Characterization and initial results from the upgraded MST interferometer-polarimeter

    Science.gov (United States)

    Ding, W. X.; Parke, E.; Brower, D. L.; Duff, J.

    2016-10-01

    The FIR interferometer-polarimeter diagnostic on MST is a high-bandwidth system with unique capabilities for measuring high-frequency density and internal magnetic fluctuations. Installation of new planar-diode mixers improves both the signal strength and the noise floor compared to the corner-cube mixers previously used. Measurements of density and Faraday rotation angle in standard reversed-field pinch (RFP) plasmas and plasmas with improved, tokamak-like confinement are presented. The noise floor in the Faraday rotation power spectrum is reduced by nearly an order of magnitude, with fluctuations observed up to 250 kHz. Cross-correlation between multiple mixers is an additional, novel technique for reducing the noise floor and improving the resolution of high-frequency, small-amplitude magnetic and density fluctuations. Correlation of signals from two independent mixers viewing the same chord reduces the noise floor by another order of magnitude. High wavenumber resolution may be possible when operating without focusing elements, using only the 2-3 mm aperture on the mixer to determine the sampled chord width. This configuration will provide better resolution of small-scale fluctuations observed in the RFP during periods of improved confinement. Work supported by U.S. D.O.E.

  2. MST - CEARÁ, 20 ANOS DE MARCHAS

    Directory of Open Access Journals (Sweden)

    Francisco Amaro Gomes de Alencar

    2010-01-01

    Full Text Available Este trabajo pretende hacer un rescate geográfi co e histórico de los veinte años del "Movimiento de los Trabajadores Rurales Sin Tierra" en Ceará (MST- CE, 1989 a 2009. Durante esos años, varias fueron las formas de luchas para que la espacialización y la territorialización del MST que sucedieron en Ceará. Algunas de esas formas de luchas fueron: los confl ictos; las ocupaciones de tierras, de los edifi cios públicos y de las avenidas; las marchas, la presencia de los militantes, en los asentamientos federal y estaduales, de los varios programas de reforma agraria. Para abordar esas marchas del MST-Ceará, este texto está estructurado en 4 partes. En la primera, la "Introducción", construimos una pequeña línea del tiempo de las luchas agrarias desde las indígenas hasta la primera ocupación de tierras realizada por el Movimiento. En la segunda, "Las acciones del MST-Ceará", describimos sus principales actividades tales como: caminadas, marchas, ocupaciones de inmuebles y edifi cios públicos en el Estado. En la tercera, "Territorialización del MST en Ceará", analizamos los caminos para tornarse presente, reconocido y respetado en Ceará.

  3. Physics and optimization of plasma startup in the RFP

    Science.gov (United States)

    Mao, W.; Chapman, B. E.; Ding, W. X.; Lin, L.; Almagri, A. F.; Anderson, J. K.; Den Hartog, D. J.; Duff, J.; Ko, J.; Kumar, S. T. A.; Morton, L.; Munaretto, S.; Parke, E.; Reusch, J. A.; Sarff, J. S.; Waksman, J.; Brower, D. L.; Liu, W.

    2015-05-01

    In the tokamak and reversed-field pinch (RFP), inductively driven toroidal plasma current provides the confining poloidal magnetic field and ohmic heating power, but the magnitude and/or duration of this current is limited by the available flux swing in the poloidal field transformer. A portion of this flux is consumed during startup as the current is initiated and ramped to its final target value, and considerable effort has been devoted to understanding startup and minimizing the amount of flux consumed. Flux consumption can be reduced during startup in the RFP by increasing the toroidal magnetic field, Bti, applied to initiate the discharge, but the underlying physics is not yet entirely understood. Toward increasing this understanding, we have for the first time in the RFP employed advanced, non-invasive diagnostics on the Madison Symmetric Torus to measure the evolution of current, magnetic field, and kinetic profiles during startup. Flux consumption during startup is dominantly inductive, but we find that the inductive flux consumption drops as Bti increases. The resistive consumption of flux, while relatively small, apparently increases with Bti due to a smaller electron temperature. However, the ion temperature increases with Bti, exceeding the electron temperature and thus reflecting non-collisional heating. Magnetic fluctuations also increase with Bti, corresponding primarily to low-n modes that emerge sequentially as the safety factor profile evolves from tokamak-like to that of the RFP.

  4. 75 FR 40857 - Webinar About Advanced Defense Technologies RFP

    Science.gov (United States)

    2010-07-14

    ... ADMINISTRATION Webinar About Advanced Defense Technologies RFP AGENCY: U.S. Small Business Administration (SBA). ACTION: Notice of open webinar meeting to discuss Advanced Defense Technologies (ADT) Request for... webinar it is hosting to answer questions from potential Offerors about the Advanced Defense...

  5. Fast ion generation and runaway through magnetic reconnection events in MST

    Science.gov (United States)

    Kim, Jungha; Anderson, Jay; Capecchi, William; Bonofiglo, Phillip; Sears, Stephanie

    2016-10-01

    Fokker-Planck and full orbit modeling are used to investigate how global reconnection events in MST plasmas generate an anisotropic fast ion distribution. A multi-step process is hypothesized. First, thermal ions are heated by a perpendicular heating mechanism, possibly a stochastic process that relies on turbulent diffusion and strong radial electric fields, or ion cyclotron damping in the tearing-driven turbulent cascade. Second, a small fraction of the heated ions have sufficient speed to develop substantial guiding center drifts that are relatively immune to stochastic magnetic transport. In the RFP, these fast ion drift orbits are favorable to confinement. Finally, these fast ions are accelerated by a parallel inductive electric field (up to 80 V/m) associated with the abruptly changing magnetic equilibrium. This strong impulsive field does not include any magnetic-fluctuation-based contribution as experienced by thermal particles or electrons, which do not run away like fast ions. CQL3D, a Fokker-Planck solver, and RIO, a full orbit tracing code, are used to model this multi-step process that is responsible for anisotropy in fast ion distribution in MST. Work supported by US DOE. Supported by US DOE.

  6. Rheology Of MonoSodium Titanate (MST) And Modified Mst (mMST) Mixtures Relevant To The Salt Waste Processing Facility

    Energy Technology Data Exchange (ETDEWEB)

    Koopman, D. C.; Martino, C. J.; Shehee, T. C.; Poirier, M. R.

    2013-07-31

    The Savannah River National Laboratory performed measurements of the rheology of suspensions and settled layers of treated material applicable to the Savannah River Site Salt Waste Processing Facility. Suspended solids mixtures included monosodium titanate (MST) or modified MST (mMST) at various solid concentrations and soluble ion concentrations with and without the inclusion of kaolin clay or simulated sludge. Layers of settled solids were MST/sludge or mMST/sludge mixtures, either with or without sorbed strontium, over a range of initial solids concentrations, soluble ion concentrations, and settling times.

  7. TRAINING POLICY OF MILITANTS MST: RESEARCH REPORTS

    Directory of Open Access Journals (Sweden)

    Suely Aparecida Martin

    2012-06-01

    Full Text Available : This article analyzes the experience of political education of MST, based on research results, held in Paraná. Tries to demonstrate how the different experiences provided by this movement to provide political formation of its members. The text is organized into three parts: the first presents the experience category develop by Edward Palmer Thompson, used as reference for analysis, the second presents a brief history of political formation in the context of MST, and highlights the latest research results. The research were conducted between 2006 and 2008, had a qualitative character. In addition to literature review , there was the reading of documents produced by the movement and interviews with members of the MST.

  8. 75 FR 40856 - Federal Register Meeting Notice; Webinar About Regional Innovation Clusters RFP

    Science.gov (United States)

    2010-07-14

    ... ADMINISTRATION Federal Register Meeting Notice; Webinar About Regional Innovation Clusters RFP AGENCY: U.S. Small Business Administration (SBA) ACTION: Notice of open webinar meeting to discuss Regional Innovation... Innovation Clusters RFP. For more information please go to http://www.sba.gov/clusters/index.html . The RFP...

  9. ARC Code TI: Mission Simulation ToolKit (MST)

    Data.gov (United States)

    National Aeronautics and Space Administration — The MST is a simulation framework, supporting the development of autonomy technology for planetary exploration vehicles. The MST provides a software test bed which...

  10. A Digital Signature Scheme Based on MST3 Cryptosystems

    Directory of Open Access Journals (Sweden)

    Haibo Hong

    2014-01-01

    Full Text Available As special types of factorization of finite groups, logarithmic signature and cover have been used as the main components of cryptographic keys for secret key cryptosystems such as PGM and public key cryptosystems like MST1, MST2, and MST3. Recently, Svaba et. al proposed a revised MST3 encryption scheme with greater security. Meanwhile, they put forward an idea of constructing signature schemes on the basis of logarithmic signatures and random covers. In this paper, we firstly design a secure digital signature scheme based on logarithmic signatures and random covers. In order to complete the task, we devise a new encryption scheme based on MST3 cryptosystems.

  11. Incorporating Software Requirements into the System RFP: Survey of RFP Language for Software by Topic, v. 2.0

    Science.gov (United States)

    2009-05-01

    Bergey 2005]. EXAMPLE 2 The contractor will development and document scenarios required to conduct architecture evaluation using the method...requirements that are part of the RFP [ Bergey 2002]. The statement of work (SOW) describes what the supplier must accomplish. In terms of any evaluation...and the potential system supplier [ Bergey 2002]. 7.14.2 Section M - Evaluation EXAMPLE 1 To incorporate architecture evaluation, Section M must

  12. MST e-News June 2016

    Energy Technology Data Exchange (ETDEWEB)

    Kippen, Karen Elizabeth [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2016-06-17

    This is Los Alamos National Laboratory's (LANL) June 2016 newsletter of the Materials Science and Technology Division. The following are major topics in this newsletter: MST-8 scientists guide national efforts to overcome nuclear energy technical challenges, first-ever scanning probe microscopy capabilities for plutonium, laboratory metallurgists make thorium targets for production of cancer-fighting isotopes, and a spotlight on Veronica Livescu.

  13. TESTING AND CHARACTERIZATION OF ENGINEERED FORMS OF MONOSODIUM TITANATE (MST)

    Energy Technology Data Exchange (ETDEWEB)

    Taylor-Pashow, K.; Nash, C.; Hobbs, D.

    2012-05-14

    Engineered forms of MST and mMST were prepared at ORNL using an internal gelation process. Samples of these two materials were characterized at SRNL to examine particle size and morphology, peroxide content, tapped densities, and Na, Ti, and C content. Batch contact tests were also performed to examine the performance of the materials. The {sup E}mMST material was found to contain less than 10% of the peroxide found in a freshly prepared batch of mMST. This was also evidenced in batch contact testing with both simulated and actual waste, where little difference in performance was seen between the two engineered materials, {sup E}MST and {sup E}mMST. Based on these results, attempts were made to increase the peroxide content of the materials by post-treatment with hydrogen peroxide. The peroxide treatment resulted in a slight ({approx}10%) increase in peroxide content; however, the peroxide:Ti molar ratio was still much lower ({approx}0.1 X) than what is seen in a freshly prepared batch of mMST. Testing with simulated waste showed the performance of the peroxide treated materials was improved. Batch contact tests were also performed with an earlier (2003) prepared lot of {sup E}MST to examine the effect of ionic strength on the performance of the material. In general the results showed a decrease in removal performance with increasing ionic strength, which is consistent with previous testing with MST. A Sr loading isotherm was also determined, and the {sup E}MST material was found to reach a Sr loading as high as 13.2 wt % after 100 days of contact at a phase ratio of 20000 mL/g. At the typical MST phase ratio of 2500 mL/g (0.4 g/L), a Sr loading of 2.64 wt % was reached after 506 hours of contact. Samples of {sup E}MST and the post-peroxide treated {sup E}mMST were also tested in a column configuration using simulated waste solution. The breakthrough curves along with analysis of the sorbent beds at the conclusion of the experiments showed that the peroxide treated

  14. MST radar and polarization lidar observations of tropical cirrus

    Directory of Open Access Journals (Sweden)

    Y. Bhavani Kumar

    Full Text Available Significant gaps in our understanding of global cirrus effects on the climate system involve the role of frequently occurring tropical cirrus. Much of the cirrus in the atmosphere is largely due to frequent cumulus and convective activity in the tropics. In the Indian sub-tropical region, the deep convective activity is very prominent from April to December, which is a favorable period for the formation of deep cumulus clouds. The fibrous anvils of these clouds, laden with ice crystals, are one of the source mechanisms for much of the cirrus in the atmosphere. In the present study, several passages of tropical cirrus were investigated by simultaneously operating MST radar and a co-located polarization lidar at the National MST Radar Facility (NMRF, Gadanki (13.45° N, 79.18° E, India to understand its structure, the background wind field and the microphysics at the cloud boundaries. The lidar system used is capable of measuring the degree of depolarization in the laser backscatter. It has identified several different cirrus structures with a peak linear depolarization ratio (LDR in the range of 0.1 to 0.32. Simultaneous observations of tropical cirrus by the VHF Doppler radar indicated a clear enhancement of reflectivity detected in the vicinity of the cloud boundaries, as revealed by the lidar and are strongly dependent on observed cloud LDR. An inter-comparison of radar reflectivity observed for vertical and oblique beams reveals that the radar-enhanced reflectivity at the cloud boundaries is also accompanied by significant aspect sensitivity. These observations indicate the presence of anisotropic turbulence at the cloud boundaries. Radar velocity measurements show that boundaries of cirrus are associated with enhanced horizontal winds, significant vertical shear in the horizontal winds and reduced vertical velocity. Therefore, these measurements indicate that a circulation at the cloud boundaries suggest an entrainment taking place close to

  15. Preface: Recent progress from networked studies based around MST radar

    Science.gov (United States)

    Hocking, Wayne K.; Lehmann, Volker; Singer, Werner; Yamamoto, Masayuki

    2014-10-01

    Studies of the mesosphere, stratosphere, and troposphere by radar, application of networks, and multi-instrument studies have grown significantly in recent years, and have covered a wide range of areas in technology, fundamental research, and application. This special issue of the Journal of Atmospheric and Solar-Terrestrial Physics on "Recent progress from networked studies based around MST radar" focuses primarily on selected papers presented at the 13th International Workshops on Scientific and Technical Aspects of MST Radar (MST13).

  16. Gradient Gene Algorithm: a Fast Optimization Method to MST Problem

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    The extension of Minimum Spanning Tree(MST) problem is an NP hardproblem which does not exit a polynomial time algorithm. In this paper, a fast optimizat ion method on MST problem--the Gradient Gene Algorithm is introduced. Compar ed with other evolutionary algorithms on MST problem, it is more advanced: firstly, very simple and easy to realize; then, efficient and accurate; finally general on other combination optimization problems.

  17. High Power Antenna Design for Lower Hybrid Current Drive in MST

    Science.gov (United States)

    Thomas, M. A.; Goetz, J. A.; Kaufman, M. C.; Oliva, S. P.; Caughman, J. B. O.; Ryan, P. M.

    2003-10-01

    RF current drive has been proposed as a method for reducing the tearing fluctuations that are responsible for anomalous energy transport in the RFP. A system for launching lower hybrid slow waves at 800 MHz and n_||= 7.5 is now in operation at up to 50 kW on MST. The antenna is an enclosed interdigital line using λ/4 resonators with an opening in the cavity through which the wave is coupled to the plasma. It has an untuned VSWR of ˜2, and is instrumented on 5 of its 23 elements to allow measurement of damping length. The antenna design is being optimized for higher power handling. Improvements include larger vacuum feedthroughs, better impedance matching, and RF instrumentation on all resonators. The new antenna will be modeled in Microwave Studio^TM. The goal is a design which can handle ˜250 kW and presents a VSWR of 1.4 or better without external tuning. Full instrumentation will allow more detailed power deposition measurements.

  18. Initial Studies of Validation of MHD Models for MST Reversed Field Pinch Plasmas

    Science.gov (United States)

    Jacobson, C. M.; Almagri, A. F.; Craig, D.; McCollam, K. J.; Reusch, J. A.; Sauppe, J. P.; Sovinec, C. R.; Triana, J. C.

    2015-11-01

    Quantitative validation of visco-resistive MHD models for RFP plasmas takes advantage of MST's advanced diagnostics. These plasmas are largely governed by MHD relaxation activity, so that a broad range of validation metrics can be evaluated. Previous nonlinear simulations using the visco-resistive MHD code DEBS at Lundquist number S = 4 ×106 produced equilibrium relaxation cycles in qualitative agreement with experiment, but magnetic fluctuation amplitudes b~ were at least twice as large as in experiment. The extended-MHD code NIMROD previously suggested that a two-fluid model may be necessary to produce b~ in agreement with experiment. For best comparisons with DEBS and to keep computational expense tractable, NIMROD is run in single-fluid mode at low S. These simulations are complemented by DEBS at higher S in cylindrical geometry, which will be used to examine b~ as a function of S. Experimental measurements are used with results from these simulations to evaluate validation metrics. Convergence tests of previous high S DEBS simulations are also discussed, along with benchmarking of DEBS and NIMROD with the SPECYL and PIXIE3D codes. Work supported by U.S. DOE and NSF.

  19. The plasma boundary in Single Helical Axis RFP plasmas

    CERN Document Server

    Martines, E; Momo, B; Munaretto, S; Innocente, P; Spolaore, M

    2009-01-01

    Single Helical Axis (SHAx) states obtained in high current reversed field pinch (RFP) plasmas display, aside from a dominant mode in the m=1 spectrum, also a dominant m=0 mode, with the same toroidal mode number as the m=1 one. The two modes have a fixed phase relationship. The island chain created by the m=0 mode across the reversal surface gives rise, at shallow reversal of the toroidal field, to an X-point structure which separates the last closed flux surface from the first wall, creating a divertor-like configuration. The plasma-wall interaction is found to be related to the connection length of the field lines intercepting the wall, which displays a pattern modulated by the dominant mode toroidal periodicity. This configuration, which occurs only for shallow toroidal field reversal, could be exploited to realize an island divertor in analogy to stellarators.

  20. Sugar exchanges in arbuscular mycorrhiza: RiMST5 and RiMST6, two novel Rhizophagus irregularis monosaccharide transporters, are involved in both sugar uptake from the soil and from the plant partner.

    Science.gov (United States)

    Ait Lahmidi, Nassima; Courty, Pierre-Emmanuel; Brulé, Daphnée; Chatagnier, Odile; Arnould, Christine; Doidy, Joan; Berta, Graziella; Lingua, Guido; Wipf, Daniel; Bonneau, Laurent

    2016-10-01

    Arbuscular mycorrhizal (AM) fungi are associated with about 80% of land plants. AM fungi provide inorganic nutrients to plants and in return up to 20% of the plant-fixed CO2 is transferred to the fungal symbionts. Since AM fungi are obligate biotrophs, unraveling how sugars are provided to the fungus partner is a key for understanding the functioning of the symbiosis. In this study, we identified two new monosaccharide transporters from Rhizophagus irregularis (RiMST5 and RiMST6) that we characterized as functional high affinity monosaccharide transporters. RiMST6 was characterized as a glucose specific, high affinity H(+) co-transporter. We provide experimental support for a primary role of both RiMST5 and RiMST6 in sugar uptake directly from the soil. The expression patterns of RiMSTs in response to partial light deprivation and to interaction with different host plants were investigated. Expression of genes coding for RiMSTs was transiently enhanced after 48 h of shading and was unambiguously dependent on the host plant species. These results cast doubt on the 'fair trade' principle under carbon-limiting conditions. Therefore, in light of these findings, the possible mechanisms involved in the modulation between mutualism and parasitism in plant-AM fungus interactions are discussed. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  1. Rfp-Y region polymorphism and Marek's disease resistance in multitrait immunocompetence-selected chicken lines.

    Science.gov (United States)

    Lakshmanan, N; Lamont, S J

    1998-04-01

    Although the influence of the chicken classical MHC in resistance to many diseases is well established, the role of the recently identified, genetically independent, MHC-like region known as Rfp-Y is unclear. The objectives of this study were to analyze the frequencies of DNA polymorphisms of the Rfp-Y region in White Leghorn lines, which were divergently selected in replicate for multitrait immunocompetence, and to determine the association of these polymorphisms with Marek's disease (MD) resistance. Chicks, either with or without herpes virus of turkey (HVT) vaccination, were challenged with 500 ffu of a very virulent Marek's disease virus (Md5) at 2 d of age. The MD-related data were collected for 10 wk. PvuII-digested genomic DNA was hybridized with an Rfp-Y region-specific probe, 18.1. Three Rfp-Y polymorphisms were observed. The frequency of one Rfp-Y polymorphism was significantly different between divergently selected multitrait immunocompetence lines in one replicate only; therefore, the impact of multitrait immunocompetence selection on Rfp-Y polymorphisms is inconclusive. The PvuII defined Rfp-Y region polymorphisms had no association with either innate or vaccine-induced MD resistance to Md5 virus challenge.

  2. RFP for Smiles and Maxi projects to the Internationale Space Station

    DEFF Research Database (Denmark)

    Denver, Troelz; Thuesen, Gøsta; Jørgensen, Finn E

    1999-01-01

    The document for describes the functionality, the performance and the requirements of the ASC Star Tracker and it includes the RFP for the project's Smiles and Maxi on the International Space Station.......The document for describes the functionality, the performance and the requirements of the ASC Star Tracker and it includes the RFP for the project's Smiles and Maxi on the International Space Station....

  3. RFP for Smiles and Maxi projects to the Internationale Space Station

    DEFF Research Database (Denmark)

    Denver, Troelz; Thuesen, Gøsta; Jørgensen, Finn E

    1999-01-01

    The document for describes the functionality, the performance and the requirements of the ASC Star Tracker and it includes the RFP for the project's Smiles and Maxi on the International Space Station.......The document for describes the functionality, the performance and the requirements of the ASC Star Tracker and it includes the RFP for the project's Smiles and Maxi on the International Space Station....

  4. First molecular identification of the transgene red fluorescent protein (RFP) in transgenic ornamental zebrafish (Danio rerio) introduced in Peru

    OpenAIRE

    Carlos Scotto; Fernando Serna

    2013-01-01

    In this paper the transgenic fluorescent red, orange and pink zebra fish (Danio rerio), found in local aquariums in Peru, were identified using the PCR technique to amplify the transgene RFP sea anemone belonging to Discosoma spp. The gene expression of the red fluorescent protein (RFP) transgene was found to determine different gradients-of-bioluminescence (shades in color) in each GMO fish analyzed. We performed sequence analysis of the two variants of the RFP along with six variants of the...

  5. New chicken Rfp-Y haplotypes on the basis of MHC class II RFLP and MLC analyses

    DEFF Research Database (Denmark)

    Juul-Madsen, H R; Zoorob, R; Auffray, C;

    1997-01-01

    New chicken Rfp-Y haplotypes were determined by the use of restriction fragment length polymorphism (RFLP) and mixed lymphocyte culture (MLC) in four different chicken haplotypes, B15, B19, B21, B201. The RFLP polymorphism was mapped to the Rfp-Y system by the use of a subclone (18.1) which maps...... near a polymorphic lectin gene located in the Rfp-Y system and DNA from families with known segregation of the implicated RFLP polymorphism. For the first time it is shown that major histocompatibility complex class II genes in the Rfp-Y system have functional implications. Sequence information...

  6. Assessment of performing an MST strike in Tank 21H

    Energy Technology Data Exchange (ETDEWEB)

    Poirier, Michael R.

    2014-09-29

    Previous Savannah River National Laboratory (SRNL) tank mixing studies performed for the Small Column Ion Exchange (SCIX) project have shown that 3 Submersible Mixer Pumps (SMPs) installed in Tank 41 are sufficient to support actinide removal by MST sorption as well as subsequent resuspension and removal of settled solids. Savannah River Remediation (SRR) is pursuing MST addition into Tank 21 as part of the Large Tank Strike (LTS) project. The preliminary scope for LTS involves the use of three standard slurry pumps (installed in N, SE, and SW risers) in a Type IV tank. Due to the differences in tank size, internal interferences, and pump design, a separate mixing evaluation is required to determine if the proposed configuration will allow for MST suspension and strontium and actinide sorption. The author performed the analysis by reviewing drawings for Tank 21 [W231023] and determining the required cleaning radius or zone of influence for the pumps. This requirement was compared with previous pilot-scale MST suspension data collected for SCIX that determined the cleaning radius, or zone of influence, as a function of pump operating parameters. The author also reviewed a previous Tank 50 mixing analysis that examined the ability of standard slurry pumps to suspend sludge particles. Based on a review of the pilot-scale SCIX mixing tests and Tank 50 pump operating experience, three standard slurry pumps should be able to suspend sludge and MST to effectively sorb strontium and actinides onto the MST. Using the SCIX data requires an assumption about the impact of cooling coils on slurry pump mixing. The basis for this assumption is described in this report. Using the Tank 50 operating experience shows three standard slurry pumps should be able to suspend solids if the shear strength of the settled solids is less than 160 Pa. Because Tank 21 does not contain cooling coils, the shear strength could be larger.

  7. Critical parameters and TCLP performance of the RFP microwave solidification system

    Energy Technology Data Exchange (ETDEWEB)

    Sprenger, G.S.

    1993-01-01

    Two series of experiments were conducted at Rocky Flats Plant (RFP) to identify the critical operating parameters for microwave solidification and to evaluate the performance of the product against the EPA's Toxicity Characteristic Leach Procedure (TCLP). A surrogate hydroxide coprecipitation sludge spiked with heavy metals was used in the study. The RFP process uses microwave energy to heat and melt the waste into a vitreous final form that is suitable for land disposal. The results of the study indicate that waste loading and borax content in the glass forming frit are critical in the treatment of hydroxide sludge. Also, the product will easily satisfy EPA's limitations for land disposal. These results are very encouraging and support RFP's commitment to the use of microwave technology for treatment of various mixed waste streams at the facility.

  8. The effectiveness of Multisystemic Therapy (MST) : A meta-analysis

    NARCIS (Netherlands)

    van der Stouwe, Trudy; Asscher, Jessica J.; Stams, Geert Jan J. M.; Dekovic, Maja; van der Laan, Peter H.

    2014-01-01

    Multisystemic Therapy (MST) is a well-established intervention for juvenile delinquents and/or adolescents showing social, emotional and behavioral problems. A multilevel meta-analysis of k = 22 studies, containing 332 effect sizes, consisting of N = 4066 juveniles, was conducted to examine the effe

  9. The effectiveness of Multisystemic Therapy (MST): a meta-analysis

    NARCIS (Netherlands)

    T. van der Stouwe; J.J. Asscher; G.J.J.M. Stams; M. Deković; P.H. van der Laan

    2014-01-01

    Multisystemic Therapy (MST) is a well-established intervention for juvenile delinquents and/or adolescents showing social, emotional and behavioral problems. A multilevel meta-analysis of k = 22 studies, containing 332 effect sizes, consisting of N = 4066 juveniles, was conducted to examine the effe

  10. Glyceraldehyde-3-phosphate dehydrogenase interacts with proapoptotic kinase mst1 to promote cardiomyocyte apoptosis.

    Directory of Open Access Journals (Sweden)

    Bei You

    Full Text Available Mammalian sterile 20-like kinase 1 (Mst1 is a critical component of the Hippo signaling pathway, which regulates a variety of biological processes ranging from cell contact inhibition, organ size control, apoptosis and tumor suppression in mammals. Mst1 plays essential roles in the heart disease since its activation causes cardiomyocyte apoptosis and dilated cardiomyopathy. However, the mechanism underlying Mst1 activation in the heart remains unknown. In a yeast two-hybrid screen of a human heart cDNA library with Mst1 as bait, glyceraldehyde-3-phosphate dehydrogenase (GAPDH was identified as an Mst1-interacting protein. The interaction of GAPDH with Mst1 was confirmed by co-immunoprecipitation in both co-transfected HEK293 cells and mouse heart homogenates, in which GAPDH interacted with the kinase domain of Mst1, whereas the C-terminal catalytic domain of GAPDH mediated its interaction with Mst1. Moreover, interaction of Mst1 with GAPDH caused a robust phosphorylation of GAPDH and markedly increased the Mst1 activity in cells. Chelerythrine, a potent inducer of apoptosis, substantially increased the nuclear translocation and interaction of GAPDH and Mst1 in cardiomyocytes. Overexpression of GAPDH significantly augmented the Mst1 mediated apoptosis, whereas knockdown of GAPDH markedly attenuated the Mst1 activation and cardiomyocyte apoptosis in response to either chelerythrine or hypoxia/reoxygenation. These findings reveal a novel function of GAPDH in Mst1 activation and cardiomyocyte apoptosis and suggest that disruption of GAPDH interaction with Mst1 may prevent apoptosis related heart diseases such as heart failure and ischemic heart disease.

  11. The RFP Process: Effective Management of the Acquisition of Library Materials.

    Science.gov (United States)

    Wilkinson, Frances C.; Thorson, Connie Capers

    Many librarians view procurement, with its myriad forms, procedures, and other organizational requirements, as a tedious or daunting challenge. This book simplifies the process, showing librarians how to successfully prepare a Request for Proposal (RFP) and make informed decisions when determining which vendors to use for purchasing library…

  12. Initial results from the rebuilt EXTRAP T2R RFP device

    Science.gov (United States)

    Brunsell, P. R.; Bergsåker, H.; Cecconello, M.; Drake, J. R.; Gravestijn, R. M.; Hedqvist\\ad{2 }, A.; Malmberg, J.-A.

    2001-11-01

    The EXTRAP T2R thin shell reversed-field pinch (RFP) device has recently resumed operation after a major rebuild including the replacement of the graphite armour with molybdenum limiters, a fourfold increase of the shell time constant, and the replacement of the helical coil used for the toroidal field with a conventional solenoid-type coil. Wall-conditioning using hydrogen glow discharge cleaning was instrumental for successful RFP operation. Carbon was permanently removed from the walls during the first week of operation. The initial results from RFP operation with relatively low plasma currents in the range Ip = 70-100 kA are reported. RFP discharges are sustained for more than three shell times. Significant improvements in plasma parameters are observed, compared to operation before the rebuild. There is a substantial reduction in the carbon impurity level. The electron density behaviour is more shot-to-shot reproducible. The typical density is ne = 0.5-1×1019 m-3. Monitors of Hα line radiation indicate that the plasma wall interaction is more toroidally symmetric and that there is less transient gas release from the wall. The minimum loop voltage is in the range Vt = 28-35 V, corresponding to a reduction by a factor of two to three compared to the value before the rebuild.

  13. Off-the-Shelf ILS: Library Issues RFP for Integrated Library System.

    Science.gov (United States)

    Library of Congress Information Bulletin, 1997

    1997-01-01

    Describes the Request for Proposal (RFP) issued by the Library of Congress for an integrated library system to help in the areas of inventory control, acquisitions, binding, cataloging, circulation, collection development, online catalogs, preservation, serials management, production of products, and the computer year 2000 problem. (LRW)

  14. Simultaneous measurement of electron temperature and density by a line pair method in the RFP plasma

    Science.gov (United States)

    Watanabe, Masayuki; Shimizu, S.; Ogawa, H.; Shinohara, T.

    2009-11-01

    A line-pair-method has been applied for a simultaneous measurement of the electron temperature and density in ATRAS RFP plasma. Three helium spectrum lines (668nm, 706nm, 728nm) were measured during the discharge at the same time and the electron temperature and density is estimated by using a Collision-Radiation model. To get the signal of the helium impunity line from the RFP discharge, the RFP plasma in the hydrogen gas with a few mixed helium gas was formed. In the typical ATRAS RFP discharge of the plasma current of 60kA, the electron temperature was approximately 50-150 eV and the electron density is the order of 10^18 m-3. During the discharge, the change of the temperature and density are mutually related and this correlation was the almost reverse phase. The periodically change of the temperature and density were also observed. This change synchronizes with a periodically increase of the averaged toroidal magnetic field, which is caused by the toroidal rotation of the increase of the toroidal magnetic field. This rotation, which is deeply related with dynamo effect, makes the plasma energy lose and particles also diffuse toward the plasma edge. As a result, the recycling of the particle and energy are occurred at the same time.

  15. The Tukey algorithm for enhancing MST radar data

    Science.gov (United States)

    Bowhill, S. A.

    1986-01-01

    One of the most troublesome features in MSR (mesosphere stratosphere troposphere) velocity measurements is the determination of unwanted scatterer whose velocity is different from that of the surrounding atmosphere. Aircraft seen in the sidelobes of the antenna are the principal problem. Because coherent integration essentially eliminates echoes with line of sight velocities greater than 10 or 20 m/s, aircraft are seen only when their flight path is almost perpendicular to the line of sight. Then, they give large returns whose velocities may be positive or negative, and certainly different from that of the surrounding air. The glitches in the minute by minute velocity records are quite troublesome in that they may distort the statistics of the velocity. An objective way is therefore needed to remove sporadic points of this kind. For this purpose, the Tukey algorithm is appropriate and has some advantages over averaging. The Tukey algorithm, applied to a data array, uses for each data point the median of it and the two points surrounding it. If the three points form a monotonically increasing or decreasing sequence, the original point is copied with change. However, if the central point is remote from the other two, it is replaced by whichever of the two surrounding points is closest in value. The greatest effect of the Tukey algorithm is on data where the successive points are uncorrelated. Examples are given.

  16. Brain 3-Mercaptopyruvate Sulfurtransferase (3MST: Cellular Localization and Downregulation after Acute Stroke.

    Directory of Open Access Journals (Sweden)

    Heng Zhao

    Full Text Available 3-Mercaptopyruvate sulfurtransferase (3MST is an important enzyme for the synthesis of hydrogen sulfide (H2S in the brain. We present here data that indicate an exclusively localization of 3MST in astrocytes. Regional distribution of 3MST activities is even and unremarkable. Following permanent middle cerebral artery occlusion (pMCAO, 3MST was down-regulated in both the cortex and striatum, but not in the corpus collosum. It appears that the down-regulation of astrocytic 3MST persisted in the presence of astrocytic proliferation due to gliosis. Our observations indicate that 3MST is probably not responsible for the increased production of H2S following pMCAO. Therefore, cystathionine β-synthase (CBS, the alternative H2S producing enzyme in the CNS, remains as a more likely potential therapeutic target than 3MST in the treatment of acute stroke through inhibition of H2S production.

  17. Assessing in vitro stem-cell function and tracking engraftment of stem cells in ischaemic hearts by using novel iRFP gene labelling.

    Science.gov (United States)

    Wang, Yingjie; Zhou, Mi; Wang, Xiaolong; Qin, Gangjian; Weintraub, Neal L; Tang, Yaoliang

    2014-09-01

    Near-infrared fluorescence (NIRF) imaging by using infrared fluorescent protein (iRFP) gene labelling is a novel technology with potential value for in vivo applications. In this study, we expressed iRFP in mouse cardiac progenitor cells (CPC) by lentiviral vector and demonstrated that the iRFP-labelled CPC (CPC(iRFP)) can be detected by flow cytometry and fluorescent microscopy. We observed a linear correlation in vitro between cell numbers and infrared signal intensity by using the multiSpectral imaging system. CPC(iRFP) injected into the non-ischaemic mouse hindlimb were also readily detected by whole-animal NIRF imaging. We then compared iRFP against green fluorescent protein (GFP) for tracking survival of engrafted CPC in mouse ischaemic heart tissue. GFP-labelled CPC (CPC(GFP)) or CPC labelled with both iRFP and GFP (CPC(iRFP) (GFP)) were injected intramyocardially into mouse hearts after infarction. Three days after cell transplantation, a strong NIRF signal was detected in hearts into which CPC(iRFP) (GFP), but not CPC(GFP), were transplanted. Furthermore, iRFP fluorescence from engrafted CPC(iRFP) (GFP) was detected in tissue sections by confocal microscopy. In conclusion, the iRFP-labelling system provides a valuable molecular imaging tool to track the fate of transplanted progenitor cells in vivo.

  18. OSM mitigates post-infarction cardiac remodeling and dysfunction by up-regulating autophagy through Mst1 suppression.

    Science.gov (United States)

    Hu, Jianqiang; Zhang, Lei; Zhao, Zhijing; Zhang, Mingming; Lin, Jie; Wang, Jiaxing; Yu, Wenjun; Man, Wanrong; Li, Congye; Zhang, Rongqing; Gao, Erhe; Wang, Haichang; Sun, Dongdong

    2016-11-04

    The incidence and prevalence of heart failure (HF) in the world are rapidly rising possibly attributed to the worsened HF following myocardial infarction (MI) in recent years. Here we examined the effects of oncostatin M (OSM) on postinfarction cardiac remodeling and the underlying mechanisms involved. MI model was induced using left anterior descending coronary artery (LAD) ligation. In addition, cultured neonatal mouse cardiomyocytes were subjected to simulated MI. Our results revealed that OSM alleviated left ventricular remodeling, promoted cardiac function, restored mitochondrial cristae density and architecture disorders after 4weeks of MI. Enhanced autophagic flux was indicated in cardiomyocytes transduced with Ad-GFP -LC3 in the OSM treated group as compared with the MI group. OSM receptor Oβ knockout blocked the beneficial effects of OSM in postinfarction cardiac remodeling and cardiomyocytes autophagy. OSM pretreatment significantly alleviated left ventricular remodeling and dysfunction in Mst1 transgenic mice, while it failed to reverse further the postinfarction left ventricular dilatation and cardiac function in the Mst1 knockout mice. Our data revealed that OSM alleviated postinfarction cardiac remodeling and dysfunction by enhancing cardiomyocyte autophagy. OSM holds promise as a therapeutic target in treating HF after MI through Oβ receptor by inhibiting Mst1 phosphorylation.

  19. Hypermethylation of MST1 in IgG4-related autoimmune pancreatitis and rheumatoid arthritis

    Energy Technology Data Exchange (ETDEWEB)

    Fukuhara, Takataro; Tomiyama, Takashi [Division of Gastroenterology and Hepatology, The Third Department of Internal Medicine, JST CREST, Kansai Medical University, 2-5-1 Shin-machi, Hirakata, Osaka 573-1010 (Japan); Yasuda, Kaneki [Department of Urology and Andrology, Kansai Medical University, 2-5-1 Shin-machi, Hirakata, Osaka 573-1010 (Japan); Ueda, Yoshihiro [Department of Molecular Genetics, Institute of Biomedical Science, and JST CREST, Kansai Medical University, 2-5-1 Shin-machi, Hirakata, Osaka 573-1010 (Japan); Ozaki, Yoshio; Son, Yonsu; Nomura, Shosaku [Department of the First Department of Internal Medicine, Kansai Medical University, 2-5-1 Shin-machi, Hirakata, Osaka 573-1010 (Japan); Uchida, Kazushige; Okazaki, Kazuichi [Division of Gastroenterology and Hepatology, The Third Department of Internal Medicine, JST CREST, Kansai Medical University, 2-5-1 Shin-machi, Hirakata, Osaka 573-1010 (Japan); Kinashi, Tatsuo, E-mail: kinashi@takii.kmu.ac.jp [Department of Molecular Genetics, Institute of Biomedical Science, and JST CREST, Kansai Medical University, 2-5-1 Shin-machi, Hirakata, Osaka 573-1010 (Japan)

    2015-08-07

    The serine/threonine kinase Mst1 plays important roles in the control of immune cell trafficking, proliferation, and differentiation. Previously, we reported that Mst1 was required for thymocyte selection and regulatory T-cell functions, thereby the prevention of autoimmunity in mice. In humans, MST1 null mutations cause T-cell immunodeficiency and hypergammaglobulinemia with autoantibody production. RASSF5C(RAPL) is an activator of MST1 and it is frequently methylated in some tumors. Herein, we investigated methylation of the promoter regions of MST1 and RASSF5C(RAPL) in leukocytes from patients with IgG4-related autoimmune pancreatitis (AIP) and rheumatoid arthritis (RA). Increased number of CpG methylation in the 5′ region of MST1 was detected in AIP patients with extrapancreatic lesions, whereas AIP patients without extrapancreatic lesions were similar to controls. In RA patients, we detected a slight increased CpG methylation in MST1, although the overall number of methylation sites was lower than that of AIP patients with extrapancreatic lesions. There were no significant changes of the methylation levels of the CpG islands in the 5′ region of RASSF5C(RAPL) in leukocytes from AIP and RA patients. Consistently, we found a significantly down-regulated expression of MST1 in regulatory T cells of AIP patients. Our results suggest that the decreased expression of MST1 in regulatory T cells due to hypermethylation of the promoter contributes to the pathogenesis of IgG4-related AIP. - Highlights: • Mst1 controls immune cells trafficking, cell proliferation and differentiation. • Autoimmune pancreatitis (AIP) is an idiopathic pancreatitis affecting multiple organs. • Decreased MST1 expression and increased CpG methylation of promoter of MST1 in AIP. • Slight increased CpG methylation of MST1 in rheumatoid arthritis patients. • MST1 contributes pathogenesis of IgG4-related AIP.

  20. Mst1-FoxO signaling protects Naive T lymphocytes from cellular oxidative stress in mice.

    Directory of Open Access Journals (Sweden)

    Juhyun Choi

    Full Text Available BACKGROUND: The Ste-20 family kinase Hippo restricts cell proliferation and promotes apoptosis for proper organ development in Drosophila. In C. elegans, Hippo homolog also regulates longevity. The mammalian Ste20-like protein kinase, Mst1, plays a role in apoptosis induced by various types of apoptotic stress. Mst1 also regulates peripheral naïve T cell trafficking and proliferation in mice. However, its functions in mammals are not fully understood. METHODOLOGY/PRINCIPAL FINDINGS: Here, we report that the Mst1-FoxO signaling pathway plays a crucial role in survival, but not apoptosis, of naïve T cells. In Mst1(-/- mice, peripheral T cells showed impaired FoxO1/3 activation and decreased FoxO protein levels. Consistently, the FoxO targets, Sod2 and catalase, were significantly down-regulated in Mst1(-/- T cells, thereby resulting in elevated levels of intracellular reactive oxygen species (ROS and induction of apoptosis. Expression of constitutively active FoxO3a restored Mst1(-/- T cell survival. Crossing Mst1 transgenic mice (Mst1 Tg with Mst1(-/- mice reduced ROS levels and restored normal numbers of peripheral naïve T cells in Mst1 Tg;Mst1(-/- progeny. Interestingly, peripheral T cells from Mst1(-/- mice were hypersensitive to gamma-irradiation and paraquat-induced oxidative stresses, whereas those from Mst1 Tg mice were resistant. CONCLUSIONS/SIGNIFICANCE: These data support the hypothesis that tolerance to increased levels of intracellular ROS provided by the Mst1-FoxOs signaling pathway is crucial for the maintenance of naïve T cell homeostasis in the periphery.

  1. Expression Levels of RFP in Normal and Cancer Human Tissues via Real-time RT-PCR Detection

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Ret finger protein(RFP) is a member of the tripartite motif family, which is characterized by a conserved RING finger of motif, a B-box, and a coiled-coil domain(they are called RBCC generally). Although RFP was known to be an oncogene when its RBCC moiety was connected with a tyrosine kinase domain by DNA rearrangement, its biological function was not well defined. In this study, by using real-time RT-PCR, the RFP expressions in human and mouse normal tissues, and in the cervical squamous cell carcinoma, endometrial adenocarcinoma, gastric adenocarcinoma, esophageal squamous cell carcinoma, and brain cancer tissues were analyzed. The result of the study proved that the highest level of mRNA reverse transcription appeared in the normal testical tissue, whereas that in other normal tissues of human and mice were low. The mRNA reverse transcription level of RFP was higher in the endometrial adenocarcinoma tissue than in the cervical squamous cell carcinoma tissue; the mRNA reverse transcription level of RFP in the gastric adenocarcinoma tissue was significantly higher than that in the esophageal squamous cell carcinoma tissue. It was also found that the mRNA reverse transcription level of RFP in the brain cancer tissue was higher than that in the normal brain tissue. These results suggested that RFP could possibly be a useful molecular target for the development of new therapeutics for malignant tumors.

  2. A Structural Basis for Reversible Photoswitching of Absorbance Spectra in Red Fluorescent Protein rsTagRFP

    Energy Technology Data Exchange (ETDEWEB)

    Pletnev, Sergei; Subach, Fedor V.; Dauter, Zbigniew; Wlodawer, Alexander; Verkhusha, Vladislav V. (Einstein); (NCI)

    2012-09-05

    rsTagRFP is the first monomeric red fluorescent protein (FP) with reversibly photoswitchable absorbance spectra. The switching is realized by irradiation of rsTagRFP with blue (440 nm) and yellow (567 nm) light, turning the protein fluorescence ON and OFF, respectively. It is perhaps the most useful probe in this color class that has yet been reported. Because of the photoswitchable absorbance, rsTagRFP can be used as an acceptor in photochromic Foerster resonance energy transfer. Yellow FPs, YPet and mVenus, are demonstrated to be excellent photochromic Foerster resonance energy transfer donors for the rsTagRFP acceptor in its fusion constructs. Analysis of X-ray structures has shown that photoswitching of rsTagRFP is accompanied by cis-trans isomerization and protonation/deprotonation of the chromophore, with the deprotonated cis- and protonated trans-isomers corresponding to its ON and OFF states, respectively. Unlike in other photoswitchable FPs, both conformers of rsTagRFP chromophore are essentially coplanar. Two other peculiarities of the rsTagRFP chromophore are an essentially hydrophobic environment of its p-hydroxyphenyl site and the absence of direct hydrogen bonding between this moiety and the protein scaffold. The influence of the immediate environment on rsTagRFP chromophore was probed by site-directed mutagenesis. Residues Glu145 and His197 were found to participate in protonation/deprotonation of the chromophore accompanying the photoswitching of rsTagRFP fluorescence, whereas residues Met160 and Leu174 were shown to spatially restrict chromophore isomerization, favoring its radiative decay.

  3. Measurements of beam-driven instabilities with the upgraded MST interferometer-polarimeter

    Science.gov (United States)

    Parke, E.; Anderson, J. K.; Brower, D. L.; Ding, W. X.

    2016-10-01

    Neutral beam injection (NBI) in MST produces a core-localized fast ion population that modifies the plasma equilibrium and drives a rich variety of instabilities. These instabilities include energetic particle modes (EPMs) and Alfvénic modes that can produce an avalanche process leading to significantly enhanced fast ion transport, as well as chirping modes. The MST FIR interferometer-polarimeter system has high-bandwidth and low phase noise and has previously been used to characterize density fluctuations correlated with many of these instabilities, as well as internal magnetic fluctuations associated with the dominant, n = 5 EPM. However, many of the Alfvénic and chirping modes were too weak to observe in polarimetry measurements in previous studies. Recent upgrades to the interferometer-polarimeter have further reduced the noise floor and extended the accessible bandwidth for fluctuation measurements. Initial measurements of fast ion driven instabilities with the upgraded system will be presented. Improved measurements of internal structure may be important for understanding mode dynamics and particle transport. Work supported by U.S. D.O.E.

  4. Comparison of Beijing MST radar and radiosonde horizontal wind measurements

    Science.gov (United States)

    Tian, Yufang; Lü, Daren

    2017-01-01

    To determine the performance and data accuracy of the 50 MHz Beijing Mesosphere-Stratosphere-Troposphere (MST) radar, comparisons of radar measured horizontal winds in the height range 3-25 km with radiosonde observations were made during 2012. A total of 427 profiles and 15 210 data pairs were compared. There was very good agreement between the two types of measurement. Standard deviations of difference (mean difference) for wind direction, wind speed, zonal wind and meridional wind were 24.86° (0.77°), 3.37 (-0.44), 3.33 (-0.32) and 3.58 (-0.25) m s-1, respectively. The annual standard deviations of differences for wind speed were within 2.5-3 m s-1 at all heights apart from 10-15 km, the area of strong winds, where the values were 3-4 m s-1. The relatively larger differences were mainly due to wind field variations in height regions with larger wind speeds, stronger wind shear and the quasi-zero wind layer. A lower MST radar SNR and a lower percentage of data pairs compared will also result in larger inconsistencies. Importantly, this study found that differences between the MST radar and radiosonde observations did not simply increase when balloon drift resulted in an increase in the real-time distance between the two instruments, but also depended on spatiotemporal structures and their respective positions in the contemporary synoptic systems. In this sense, the MST radar was shown to be a unique observation facility for atmospheric dynamics studies, as well as an operational meteorological observation system with a high temporal and vertical resolution.

  5. MST1 activation by curcumin mediates JNK activation, Foxo3a nuclear translocation and apoptosis in melanoma cells

    Energy Technology Data Exchange (ETDEWEB)

    Yu, Teng, E-mail: tengyu33@yahoo.com [Department of Dermatology, Shandong Ji-ning No. 1 People’s Hospital, Shandong Province 272011 (China); Ji, Jiang [Department of Dermatology, The Second Hospital Affiliated of Soochow University, SuZhou, Jiangsu Province 215000 (China); Guo, Yong-li [Department of Oncology, Shandong Ji-ning No. 1 People’s Hospital, Shandong Province 272011 (China)

    2013-11-08

    Highlights: •Curcumin activates MST1 in melanoma cells. •MST1 mediates curcumin-induced apoptosis of melanoma cells. •ROS production is involved in curcumin-induced MST1 activation. •MST1 mediates curcumin-induced JNK activation in melanoma cells. •MST1 mediates curcumin-induced Foxo3a nuclear translocation and Bim expression. -- Abstract: Different groups including ours have shown that curcumin induces melanoma cell apoptosis, here we focused the role of mammalian Sterile 20-like kinase 1 (MST1) in it. We observed that curcumin activated MST1-dependent apoptosis in cultured melanoma cells. MST1 silencing by RNA interference (RNAi) suppressed curcumin-induced cell apoptosis, while MST1 over-expressing increased curcumin sensitivity. Meanwhile, curcumin induced reactive oxygen species (ROS) production in melanoma cells, and the ROS scavenger, N-acetyl-cysteine (NAC), almost blocked MST1 activation to suggest that ROS might be required for MST1 activation by curcumin. c-Jun N-terminal protein kinase (JNK) activation by curcumin was dependent on MST1, since MST1 inhibition by RNAi or NAC largely inhibited curcumin-induced JNK activation. Further, curcumin induced Foxo3 nuclear translocation and Bim-1 (Foxo3 target gene) expression in melanoma cells, such an effect by curcumin was inhibited by MST1 RNAi. In conclusion, we suggested that MST1 activation by curcumin mediates JNK activation, Foxo3a nuclear translocation and apoptosis in melanoma cells.

  6. Ion temperature gradient turbulence in helical and axisymmetric RFP plasmas

    CERN Document Server

    Predebon, I

    2015-01-01

    Turbulence induced by the ion temperature gradient (ITG) is investigated in the helical and axisymmetric plasma states of a reversed field pinch device by means of gyrokinetic calculations. The two magnetic configurations are systematically compared, both linearly and nonlinearly, in order to evaluate the impact of the geometry on the instability and its ensuing transport, as well as on the production of zonal flows. Despite its enhanced confinement, the high-current helical state demonstrates a lower ITG stability threshold compared to the axisymmetric state, and ITG turbulence is expected to become an important contributor to the total heat transport.

  7. Fusion core start-up, ignition and burn simulations of reversed-field pinch (RFP) reactors

    Energy Technology Data Exchange (ETDEWEB)

    Chu, Yuh-Yi

    1988-01-01

    A transient reactor simulation model is developed to investigate and simulate the start-up, ignition and burn of a reversed-field pinch reactor. The simulation is based upon a spatially averaged plasma balance model with field profiles obtained from MHD quasi-equilibrium analysis. Alpha particle heating is estimated from Fokker-Planck calculations. The instantaneous plasma current is derived from a self-consistent circuit analysis for plasma/coil/eddy current interactions. The simulation code is applied to the TITAN RFP reactor design which features a compact, high-power-density reversed-field pinch fusion system. A contour analysis is performed using the steady-state global plasma balance. The results are presented with contours of constant plasma current. A saddle point is identified in the contour plot which determines the minimum value of plasma current required to achieve ignition. An optimized start-up to ignition and burn path can be obtained by passing through the saddle point. The simulation code is used to study and optimize the start-up scenario. In the simulations of the TITAN RFP reactor, the OH-driven superconducting EF coils are found to deviate from the required equilibrium values as the induced plasma current increases. This results in the modification of superconducting EF coils and the addition of a set of EF trim coils. The design of the EF coil system is performed with the simulation code subject to the optimization of trim-coil power and current. In addition, the trim-coil design is subject to the constraints of vertical-field stability index and maintenance access. A power crowbar is also needed to prevent the superconducting EF coils from generating excessive vertical field. A set of basic results from the simulation of TITAN RFP reactor yield a picture of RFP plasma operation in a reactor. Investigations of eddy current are also presented. 145 refs., 37 figs., 2 tabs.

  8. PENERAPAN TEORI GRAF UNTUK MENYELESAIKAN MASALAH MINIMUM SPANNING TREE (MST MENGGUNAKAN ALGORITMA KRUSKAL

    Directory of Open Access Journals (Sweden)

    Swaditya Rizki

    2012-10-01

    Full Text Available One of useful graph theory to solve the real problems is Minimum Spanning Tree (MST. MST is network optimization problems that can be applied in many fields such as transportations problems and communication network design (Gruber and Raidl, 2005. MST begins from tree namely a connected graph has no circuits. From the graph, there is a sub-graph that has all the vertex or spanning tree. If that graph has the weight/cost, then the spanning tree that has the smallest weight/cost is called Minimum Spanning Tree. Basic algorithm used to determine the MST is Kruskal’s algorithm. This algorithm is known as one of the best algorithms for the optimization problems, especially for MST. In this paper is developed a source code program to determine MST using Kruskal’s algorithm and then implemented on several data representing a complete graph.

  9. Locked modes and magnetic field errors in MST

    Energy Technology Data Exchange (ETDEWEB)

    Almagri, A.F.; Assadi, S.; Prager, S.C.; Sarff, J.S.; Kerst, D.W.

    1992-06-01

    In the MST reversed field pinch magnetic oscillations become stationary (locked) in the lab frame as a result of a process involving interactions between the modes, sawteeth, and field errors. Several helical modes become phase locked to each other to form a rotating localized disturbance, the disturbance locks to an impulsive field error generated at a sawtooth crash, the error fields grow monotonically after locking (perhaps due to an unstable interaction between the modes and field error), and over the tens of milliseconds of growth confinement degrades and the discharge eventually terminates. Field error control has been partially successful in eliminating locking.

  10. Genetic deletion of Mst1 alters T cell function and protects against autoimmunity.

    Directory of Open Access Journals (Sweden)

    Konstantin V Salojin

    Full Text Available Mammalian sterile 20-like kinase 1 (Mst1 is a MAPK kinase kinase kinase which is involved in a wide range of cellular responses, including apoptosis, lymphocyte adhesion and trafficking. The contribution of Mst1 to Ag-specific immune responses and autoimmunity has not been well defined. In this study, we provide evidence for the essential role of Mst1 in T cell differentiation and autoimmunity, using both genetic and pharmacologic approaches. Absence of Mst1 in mice reduced T cell proliferation and IL-2 production in vitro, blocked cell cycle progression, and elevated activation-induced cell death in Th1 cells. Mst1 deficiency led to a CD4+ T cell development path that was biased toward Th2 and immunoregulatory cytokine production with suppressed Th1 responses. In addition, Mst1-/- B cells showed decreased stimulation to B cell mitogens in vitro and deficient Ag-specific Ig production in vivo. Consistent with altered lymphocyte function, deletion of Mst1 reduced the severity of experimental autoimmune encephalomyelitis (EAE and protected against collagen-induced arthritis development. Mst1-/- CD4+ T cells displayed an intrinsic defect in their ability to respond to encephalitogenic antigens and deletion of Mst1 in the CD4+ T cell compartment was sufficient to alleviate CNS inflammation during EAE. These findings have prompted the discovery of novel compounds that are potent inhibitors of Mst1 and exhibit desirable pharmacokinetic properties. In conclusion, this report implicates Mst1 as a critical regulator of adaptive immune responses, Th1/Th2-dependent cytokine production, and as a potential therapeutic target for immune disorders.

  11. mställdhet i förskolan : En studie om att arbeta i ett jämställdhetsprojekt

    OpenAIRE

    Björklund, Anette; Johansson, Robert

    2008-01-01

    Syftet med vår kvalitativa studie är att undersöka hur förskollärare, som har deltagit i jämställdhetsprojektet Jämställdhet i centrum, har påverkats av att delta i detta projekt. Utifrån syftet har vi följande frågeställningar: På vilket sätt har JIC förändrat verksamheten på de förskolor som har deltagit? Vad kan förskollärare göra för att arbeta för en mer jämställd förskola? För vår kvalitativa intervju har vi valt att intervjua sex förskollärare, som har deltagit i projektet Jämställdhet...

  12. Studying epigenetic interactions using MicroScale Thermophoresis (MST

    Directory of Open Access Journals (Sweden)

    Thomas Schubert

    2015-08-01

    Full Text Available Epigenetic regulation is based on specific molecular interactions between epigenetic reader, writer and eraser molecules and chromatin. Binding parameters of these interactions such as binding affinities, stoichiometries and thermodynamics are essential for the understanding of the establishment and maintenance of epigenetic networks. The MicroScale Thermophoresis (MST is a rapid and precise method to characterize epigenetic interactions in solution at microliter scale, requiring low concentrations of the potential interactors. The technology is based on the movement of molecules through temperature gradients, a physical effect referred to as thermophoresis. The thermophoretic movement of a molecule depends on its size, charge and hydration shell. Upon the interaction of two molecules, at least one of these parameters is altered, leading to a change in the movement behavior, which can be used to quantify molecular interactions. MST offers free choice of buffers, also allowing measurements in serum and crude extracts, thereby ensuring optimal reaction conditions. Binding affinities from pM to mM can be measured, perfectly suited to analyze protein/protein, protein/modified peptide and protein/nucleic acid interactions in epigenetics. This review demonstrates the potential of this rapid and versatile technology in the characterization of epigenetic modifiers.

  13. Runaway electrons and mitigation studies in MST tokamak plasmas

    Science.gov (United States)

    Goetz, J. A.; Chapman, B. E.; Almagri, A. F.; Cornille, B. S.; Dubois, A.; McCollam, K. J.; Munaretto, S.; Sovinec, C. R.

    2016-10-01

    Studies of runaway electrons generated in low-density MST tokamak plasmas are being undertaken. The plasmas have Bt resonant magnetic perturbations (RMP's). An m = 3 RMP strongly suppresses the runaway electrons and initial NIMROD modeling shows that this may be due to degradation of flux surfaces. The RMP is produced by a poloidal array of 32 saddle coils at the narrow vertical insulated cut in MST's thick conducting shell, with each RMP having a single m but a broad n spectrum. While a sufficiently strong m = 3 RMP suppresses the runaway electrons, an RMP with m = 1 and comparable amplitude has little effect. The impact of the RMP's on the magnetic topology of these plasmas is being studied with the nonlinear MHD code NIMROD. With an m = 3 RMP, stochasticity is introduced in the outer third of the plasma but no such flux surface degradation is observed with an m = 1 RMP. NIMROD also predicts regularly occurring MHD activity similar to that observed in the experiment. These studies have also been done in q (a) = 2.7 plasmas and analysis and modeling is ongoing. This work supported by USDoE.

  14. A Role for MST Neurons in Heading Estimation

    Science.gov (United States)

    Stone, Leland Scott; Perrone, J. A.; Wade, Charles E. (Technical Monitor)

    1994-01-01

    A template model of human visual self-motion perception (Perrone, JOSA, 1992; Perrone & Stone, Vis. Res., in press), which uses neurophysiologically realistic "heading detectors", is consistent with numerous human psychophysical results (Warren & Hannon, Nature, 1988; Stone & Perrone, Neuro. Abstr., 1991) including the failure of humans to estimate their heading (direction of forward translation) accurately under certain visual conditions (Royden et al., Nature, 1992). We tested the model detectors with stimuli used by others in- single-unit studies. The detectors showed emergent properties similar to those of MST neurons: 1) Sensitivity to non-preferred flow. Each detector is tuned to a specific combination of flow components and its response is systematically reduced by the addition of nonpreferred flow (Orban et al., PNAS, 1992), and 2) Position invariance. The detectors maintain their apparent preference for particular flow components over large regions of their receptive fields (e.g. Duffy & Wurtz, J. Neurophys., 1991; Graziano et al., J. Neurosci., 1994). It has been argued that this latter property is incompatible with MST playing a role in heading perception. The model however demonstrates how neurons with the above response properties could still support accurate heading estimation within extrastriate cortical maps.

  15. Characterization and Genetic Analysis of a Novel Mutant mst of Rice Defective in Flower Development

    Institute of Scientific and Technical Information of China (English)

    LI Yun; XU Pei-zhou; ZHANG Hong-yu; FU Shao-hong; YANG Jin; ZHANG Ru-quan; WU Xian-jun

    2009-01-01

    A spontaneous mutant with multiple stigmas (mst) was found in an indica rice line 466. The mst mutant exhibits normal at the vegetative development stage and produces normal inflorescence structures. The difference between the mutant and the wild type was observed when the stamen primordium began to develop. In the mst florets, palea and lemma opened, lodicules were homeotically transformed into palea/lemma-like structures, and stamens were homeotically transformed into carpel-like structures. It looked like multiple stigmas being full of the whole floret. The phenotypic changes of mst were very similar to that of B-like mutant spw1. Compared with other mutants with pistillate morphologies, the severe mst florets showed that the inner three floral organs were completely changed into palea/lemma-like structures. Moreover, the mutant was female sterile. Occasionally, with the changing environment, one or two stamens were fertile. Genetic analysis indicated that the mutant traits were controlled by a single recessive gene.

  16. First molecular identification of the transgene red fluorescent protein (RFP in transgenic ornamental zebrafish (Danio rerio introduced in Peru

    Directory of Open Access Journals (Sweden)

    Carlos Scotto

    2013-09-01

    Full Text Available In this paper the transgenic fluorescent red, orange and pink zebra fish (Danio rerio, found in local aquariums in Peru, were identified using the PCR technique to amplify the transgene RFP sea anemone belonging to Discosoma spp. The gene expression of the red fluorescent protein (RFP transgene was found to determine different gradients-of-bioluminescence (shades in color in each GMO fish analyzed. We performed sequence analysis of the two variants of the RFP along with six variants of the existing fluorescent protein GFP from the Genbank, this could help identify quickly if they are new genes or variants thereof as these novel fluorescent proteins may be introduced in aquatic GMO in the future. Thus, developing and improving biosecurity measures through its timely detection at the molecular genetic level.

  17. 20 ANOS EM MOVIMENTO. 20 ANOS DE EZLN E MST

    Directory of Open Access Journals (Sweden)

    Jorge Ramon Montenegro Gómez

    2012-04-01

    Full Text Available Ao longo dos meses de abril e maio, aconteceu na FCT/UNESP, campus de Presidente Prudente, uma série de atividades, organizadas pelo Centro de Estudos de Geografia do Trabalho – CEGeT, pelo Centro de Memória e Hemeroteca Sindical “Florestan Fernandes” – CEMOSi e pelo Núcleo de Estudos, Pesquisas e Projetos de Reforma Agrária – NERA, apresentando as lutas e resistências dos dois movimentos sociais com maior visibilidade e reconhecimento na América Latina (e, seguramente, no mundo: O Exército Zapatista de Libertação Nacional – EZLN e o Movimento dos Trabalhadores Rurais Sem Terra – MST.

  18. MST2 phosphorylation at serine 385 in mitosis inhibits its tumor suppressing activity.

    Science.gov (United States)

    Chen, Xingcheng; Chen, Yuanhong; Dong, Jixin

    2016-12-01

    Mammalian sterile 20-like kinase 1/2 (MST1/2) are core tumor suppressors in the Hippo signaling pathway. MST1/2 have been shown to regulate mitotic progression. Here, we report a novel mechanism for phospho-regulation of MST2 in mitosis and its biological significance in cancer. We found that the mitotic kinase cyclin-dependent kinase 1 (CDK1) phosphorylates MST2 in vitro and in vivo at serine 385 during antimitotic drug-induced G2/M phase arrest. This phosphorylation occurs transiently during unperturbed mitosis. Mitotic phosphorylation of MST2 does not affect its kinase activity or Hippo-YAP signaling. We further showed that mitotic phosphorylation-deficient mutant MST2-S385A possesses higher activity in suppressing cell proliferation and anchorage-independent growth in vitro and tumorigenesis in vivo. Together, our findings reveal a novel layer of regulation for MST2 in mitosis and its role in tumorigenesis. Copyright © 2016 Elsevier Inc. All rights reserved.

  19. Particle Heating and Energization During Magnetic Reconnection Events in MST Plasmas

    Science.gov (United States)

    Dubois, Ami M.; Almagri, A. F.; Anderson, J. K.; den Hartog, D. J.; Forest, C.; Nornberg, M.; Sarff, J. S.

    2015-11-01

    Magnetic reconnection plays an important role in particle transport, energization, and acceleration in space, astrophysical, and laboratory plasmas. In MST reversed field pinch plasmas, discrete magnetic reconnection events release large amounts of energy from the equilibrium magnetic field, resulting in non-collisional ion heating. However, Thomson Scattering measures a decrease in the thermal electron temperature. Recent fast x-ray measurements show an enhancement in the high energy x-ray flux during reconnection, where the coupling between edge and core tearing modes is essential for enhanced flux. A non-Maxwellian energetic electron tail is generated during reconnection, where the power law spectral index (γ) decreases from 4.3 to 1.8 and is dependent on density, plasma current, and the reversal parameter. After the reconnection event, γ increases rapidly to 5.8, consistent with the loss of energetic electrons due to stochastic thermal transport. During the reconnection event, the change in γ is correlated with the change in magnetic energy stored in the equilibrium field, indicating that the released magnetic energy may be an energy source for electron energization. Recent experimental and computational results of energetic electron tail formation during magnetic reconnection events will be presented. This work is supported by the U.S. DOE and the NSF.

  20. 23 CFR 636.402 - What types of information exchange may take place after the release of the RFP document?

    Science.gov (United States)

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false What types of information exchange may take place after... What types of information exchange may take place after the release of the RFP document? Certain types.... These communication methods are optional. Type of information exchange When Purpose Parties involved...

  1. 23 CFR 636.303 - May pre-qualification standards be used as proposal evaluation criteria in the RFP?

    Science.gov (United States)

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false May pre-qualification standards be used as proposal evaluation criteria in the RFP? 636.303 Section 636.303 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS DESIGN-BUILD CONTRACTING Proposal...

  2. Emulating the Visual Receptive Field Properties of MST Neurons with a Template Model of Heading Estimation

    Science.gov (United States)

    Perrone, John A.; Stone, Leland S.

    1997-01-01

    We have previously proposed a computational neural-network model by which the complex patterns of retinal image motion generated during locomotion (optic flow) can be processed by specialized detectors acting as templates for specific instances of self-motion. The detectors in this template model respond to global optic flow by sampling image motion over a large portion of the visual field through networks of local motion sensors with properties similar to neurons found in the middle temporal (MT) area of primate extrastriate visual cortex. The model detectors were designed to extract self-translation (heading), self-rotation, as well as the scene layout (relative distances) ahead of a moving observer, and are arranged in cortical-like heading maps to perform this function. Heading estimation from optic flow has been postulated by some to be implemented within the medial superior temporal (MST) area. Others have questioned whether MST neurons can fulfill this role because some of their receptive-field properties appear inconsistent with a role in heading estimation. To resolve this issue, we systematically compared MST single-unit responses with the outputs of model detectors under matched stimulus conditions. We found that the basic physiological properties of MST neurons can be explained by the template model. We conclude that MST neurons are well suited to support heading estimation and that the template model provides an explicit set of testable hypotheses which can guide future exploration of MST and adjacent areas within the primate superior temporal sulcus.

  3. MST1 is a novel regulator of apoptosis in pancreatic beta-cells

    Science.gov (United States)

    Ardestani, Amin; Khobragade, Vrushali; Yuan, Ting; Frogne, Thomas; Tao, Wufan; Oberholzer, Jose; Pattou, Francois; Conte, Julie Kerr; Maedler, Kathrin

    2014-01-01

    Apoptotic cell death is a hallmark of the loss of insulin producing beta-cells in all forms of diabetes mellitus. Current treatment fails to halt the decline in functional beta-cell mass. Strategies to prevent beta-cell apoptosis and dysfunction are urgently needed. Here, we identified Mammalian Sterile 20-like kinase 1 (MST1) as a critical regulator of apoptotic beta-cell death and function. MST1 was strongly activated in beta-cells under diabetogenic conditions and correlated with beta-cell apoptosis. MST1 specifically induced the mitochondrial-dependent pathway of apoptosis in beta-cells through up-regulation of the BH3-only protein Bim. MST1 directly phosphorylated PDX1 at Thr11, resulting in its ubiquitination, degradation and impaired insulin secretion. Mst1 deficiency completely restored normoglycemia, beta-cell function and survival in vitro and in vivo. We show MST1 as novel pro-apoptotic kinase and key mediator of apoptotic signaling and beta-cell dysfunction, which may serve as target for the development of novel therapies for diabetes. PMID:24633305

  4. MstX and a putative potassium channel facilitate biofilm formation in Bacillus subtilis.

    Directory of Open Access Journals (Sweden)

    Matthew E Lundberg

    Full Text Available Biofilms constitute the predominant form of microbial life and a potent reservoir for innate antibiotic resistance in systemic infections. In the spore-forming bacterium Bacillus subtilis, the transition from a planktonic to sessile state is mediated by mutually exclusive regulatory pathways controlling the expression of genes required for flagellum or biofilm formation. Here, we identify mstX and yugO as novel regulators of biofilm formation in B. subtilis. We show that expression of mstX and the downstream putative K+ efflux channel, yugO, is necessary for biofilm development in B. subtilis, and that overexpression of mstX induces biofilm assembly. Transcription of the mstX-yugO operon is under the negative regulation of SinR, a transcription factor that governs the switch between planktonic and sessile states. Furthermore, mstX regulates the activity of Spo0A through a positive autoregulatory loop involving KinC, a histidine kinase that is activated by potassium leakage. The addition of potassium abrogated mstX-mediated biofilm formation. Our findings expand the role of Spo0A and potassium homeostasis in the regulation of bacterial development.

  5. TESTING OF BASELINE AND LAMINATED FILTER DISKS USING MST AND MMST WITH A PILOT SCALE ROTARY FILTER

    Energy Technology Data Exchange (ETDEWEB)

    Herman, D.

    2001-12-19

    Testing was completed to compare the filtration performance of modified monosodium titanate (mMST) with that of monosodium titanate (MST) with the rotary microfilter. In addition, the performance of the new laminated filter disk was compared to that of the original baseline welded filter disk. Results showed that flux rates for mMST exceeded that of MST with both the baseline and laminated filter disks in deployment concentrations of 0.2 g/L of mMST and 0.4 g/L of MST. The filtration rate of the mMST with the laminated filter disk exceeded that of the baseline filter disk. However, the baseline filter disk filtration rate for MST was greater than that of the laminated disk. The measured sample turbidity for all tests was 1.06 NTU or less. A contract was established with SpinTek Filtration{trademark} to operate a 3-disk pilot scale unit with prototypic filter disks and various feeds and two different filter disk membranes. SpinTek evaluated a set of the baseline 0.5 micron filter disks as well as a set of laminated filter disks using the same 0.5 micron filter disks. The membrane used for both disk sets was manufactured by the Pall Corporation (PMM 050). Each set of disks was run with monosodium titanate (MST) and modified monosodium titanate (mMST). Throughout the testing, samples of the filtrate were collected and measured for turbidity.

  6. An upgraded x-ray spectroscopy diagnostic on MST.

    Science.gov (United States)

    Clayton, D J; Almagri, A F; Burke, D R; Forest, C B; Goetz, J A; Kaufman, M C; O'Connell, R

    2010-10-01

    An upgraded x-ray spectroscopy diagnostic is used to measure the distribution of fast electrons in MST and to determine Z(eff) and the particle diffusion coefficient D(r). A radial array of 12 CdZnTe hard-x-ray detectors measures 10-150 keV Bremsstrahlung from fast electrons, a signature of reduced stochasticity and improved confinement in the plasma. A new Si soft-x-ray detector measures 2-10 keV Bremsstrahlung from thermal and fast electrons. The shaped output pulses from both detector types are digitized and the resulting waveforms are fit with Gaussians to resolve pileup and provide good time and energy resolution. Lead apertures prevent detector saturation and provide a well-known etendue, while lead shielding prevents pickup from stray x-rays. New Be vacuum windows transmit >2 keV x-rays, and additional Al and Be filters are sometimes used to reduce low energy flux for better resolution at higher energies. Measured spectra are compared to those predicted by the Fokker-Planck code CQL3D to deduce Z(eff) and D(r).

  7. EL MST Y LAS DISPUTAS POR LAS ALTERNATIVAS EN BRASIL

    Directory of Open Access Journals (Sweden)

    João Pedro Stédile

    2012-04-01

    Full Text Available La victoria del presidente Lulaen las últimas elecciones cambió la correlaciónde fuerzas de la lucha por la reforma agraria enBrasil. Por los compromisos históricos del PT,su liderazgo y como partido de izquierda,tenemos ahora un gobierno federal que apoyala reforma agraria, al contrario de lo que fue elgobierno de Fernando Henrique Cardoso. Porlo tanto, la disputa se sitúa en otro nivel. Sinembargo, hay otras fuerzas poderosas que seoponen a la reforma agraria como lo son ellatifundio, el modelo neoliberal del agro-negocio, la clase dominante como un todo, elEstado burgués brasileño y los medios decomunicación que actúan como un espacio delucha ideológica que disputa la hegemonía en lasociedad contra nosotros. En este nuevocontexto, el MST evalúa que ahora sí se puedeavanzar en la reforma agraria, pero que es unmomento de acumulación de fuerzas y no degrandes definiciones que consoliden la reformaagraria de nuevo tipo que nosotrosdefendemos. Es decir, estamos acumulandopara el futuro.

  8. MODEL PEMBELAJARAN TAPPS BERBANTUAN MST UNTUK MENINGKATKAN KEMAMPUAN BERPIKIR KREATIF

    Directory of Open Access Journals (Sweden)

    Uun Kurniasih

    2014-06-01

    Full Text Available AbstrakTujuan penelitian ini   untuk mendeskripsi karakteristik pembelajaran model TAPPS strategi IDEP DCG berbantuan MST serta menghasilkan perangkat pembelajaran matematika yang valid, praktis dan efektif. Penelitian ini termasuk jenis R & D atau jenis penelitian pengembangan . Pengembangan perangkat pembelajaran mengacu pada model Thiagarajan, Semmel dan Semmel. Pengumpulan data terdiri dari lembar validasi, lembar  pengamatan  aktivitas  siswa,  lembar  angket  motivasi,  lembar  respon  siswa, lembar kemampuan guru melaksanakan   pembelajaran dan tes kemampuan berpikir kreatif.Hasil penelitian menunjukkan: (1 karakteristik   pembelajaran TAPPS adalah pembelajaran efektif, menyenangkan, inovatif, serta menumbuhkembangkan semangat kepercayaan diri siswa dalam memunculkan ide baru sehingga mampu memecahkan permasalahan  secara  sistematis  ;  (2  perangkat  pembelajaran  yang  dikembangkan valid; dengan kriteria silabus, RPP, LKS, buku siswa, dan TKBK masing-masing sangat baik; (3 Perangkat pembelajarannya praktis, yaitu: a kemampuan guru melaksanakan pembelajaran baik mempunyai rata-rata 4,395; dan b respon siswa positif mempunyai rata-rata  86%; (4  pembelajaran  matematika efektif  , data penelitian  menunjukkan ketuntasan belajar  melebihi 75%, adanya pengaruh positif aktivitas dan motivasi siswa terhadap ketuntasan berpikir kreatif  sebesar 86,2%;   berdasarkan uji banding kelas ekperimen lebih baik dari pada kelas ekspositori; adanya peningkatan kemampuan menjawab soal secara sistematis/tepat (fluency, memberikan jawaban yang beragam (flexibility, dan jawabannya menggunakan bahasa atau ide sendiri (orisinalitty.AbstractThe purpose of this research is to study characteristics of  model TAPPS with IDEP DCG strategy aided MST and produce valid mathematical learning tools, practical and effective. This research includes type of research development. Learning software development refers to model Thiagarajan

  9. Program of the Antarctic Syowa MST/IS radar (PANSY)

    Science.gov (United States)

    Sato, Kaoru; Tsutsumi, Masaki; Sato, Toru; Nakamura, Takuji; Saito, Akinori; Tomikawa, Yoshihiro; Nishimura, Koji; Kohma, Masashi; Yamagishi, Hisao; Yamanouchi, Takashi

    2014-10-01

    The PANSY radar is the first Mesosphere-Stratosphere-Troposphere/Incoherent Scatter (MST/IS) radar in the Antarctic region. It is a large VHF monostatic pulse Doppler radar operating at 47 MHz, consisting of an active phased array of 1045 Yagi antennas and an equivalent number of transmit-receive (TR) modules with a total peak output power of 500 kW. The first stage of the radar was installed at Syowa Station (69°00‧S, 39°35‧E) in early 2011, and is currently operating with 228 antennas and modules. This paper reports the project's scientific objectives, technical descriptions, and the preliminary results of observations made to date. The radar is designed to clarify the role of atmospheric gravity waves at high latitudes in the momentum budget of the global circulation in the troposphere, stratosphere and mesosphere, and to explore the dynamical aspects of unique polar phenomena such as polar mesospheric clouds (PMC) and polar stratospheric clouds (PSC). The katabatic winds as a branch of Antarctic tropospheric circulation and as an important source of gravity waves are also of special interest. Moreover, strong and sporadic energy inputs from the magnetosphere by energetic particles and field-aligned currents can be quantitatively assessed by the broad height coverage of the radar which extends from the lower troposphere to the upper ionosphere. From engineering points of view, the radar had to overcome restrictions related to the severe environments of Antarctic research, such as very strong winds, limited power availability, short construction periods, and limited manpower availability. We resolved these problems through the adoption of specially designed class-E amplifiers, light weight and tough antenna elements, and versatile antenna arrangements. Although the radar is currently operating with only about a quarter of its full designed system components, we have already obtained interesting results on the Antarctic troposphere, stratosphere and

  10. Mean winds observed with Indian MST radar over tropical mesosphere and comparison with various techniques

    Energy Technology Data Exchange (ETDEWEB)

    Venkat Ratnam, M.; Narayana Rao, D.; Narayana Rao, T. [Sri Venkateswara Univ., Tirupati (India). Dept. of Physics; Thulasiraman, S.; Nee, J.B. [National Central Univ., Chung Li (Taiwan); Gurubaran, S.; Rajaram, R. [Equatorial Geophysical Research Lab., Indian Inst. of Geomagnetism, Tirunelveli (India)

    2001-08-01

    Temporal variation of mean winds between the 65 to 85 km height region from the data collected over the course of approximately four years (1995-99), using the Indian MST radar located at Gadanki (13.5 N, 79.2 E), India is presented in this paper. Mesospheric mean winds and their seasonal variation in the horizontal and vertical components are presented in detail. Westward flows during each of the equinoxes and eastward flows during the solstices are observed in the zonal component. The features of the semi-annual oscillation (SAO) and the quasi-biennial oscillation (QBO) in the zonal component are noted. In the meridional component, contours reveal a northward motion during the winter and a southward motion during the summer. Large inter-annual variability is found in the vertical component with magnitudes of the order of {+-}2 ms{sup -1}. The MST observed winds are also compared with the winds observed by the MF radar located at Tirunelveli (8.7 N, 77.8 E), India, the high resolution Doppler imager (HRDI) onboard the upper atmospheric research satellite (UARS), and with the CIRA-86 model. A very good agreement is found between both the ground-based instruments (MST radar and MF radar) in the zonal component and there are few discrepancies in the meridional component. UARS/HRDI observed winds usually have larger magnitudes than the ground-based mean winds. Comparison of the MST derived winds with the CIRA-86 model in the zonal component shows that during the spring equinox and the summer, the winds agree fairly well, but there are a lot of discrepancies in the other seasons and the observed winds with the MST radar are less in magnitude, though the direction is same. The strengths and limitations in estimating reliable mesospheric mean winds using the MST radar are also discussed. (orig.)

  11. STRONTIUM AND ACTINIDE SORPTION BY MST AND MMST UNDER CONDITIONS REVELANT TO THE SMALL COLUMN ION-EXCHANGE PROCESS

    Energy Technology Data Exchange (ETDEWEB)

    Taylor-Pashow, K.; Hobbs, D.; Poirier, M.

    2011-05-06

    A series of tests were performed to examine the kinetics of Sr and actinide removal by monosodium titanate (MST) and modified monosodium titanate (mMST) under mixing conditions similar to what will be provided in the Small Column Ion Exchange (SCIX) Program. Similar removal kinetics were seen for two different mixing energies, indicating that under these conditions bulk solution transport is not the rate limiting step for Sr and actinide removal. Sr removal was found to be rapid for both MST and mMST, reaching steady-state conditions within six hours. In contrast, at least six weeks is necessary to reach steady-state conditions for Pu with MST. For mMST, steady-state conditions for Pu were achieved within two weeks. The actual contact time required for the SCIX process will depend on starting sorbate concentrations as well as the requirements for the decontaminated salt solution. During testing leaks occurred in both the MST and mMST tests and evidence of potential desorption was observed. The desorption likely occurred as a result of the change in solids to liquid phase ratio that occurred due to the loss of solution. Based on these results, Savannah River National Laboratory (SRNL) recommended additional testing to further study the effect of changing phase ratios on desorption. This testing is currently in progress and results will be documented in a separate report.

  12. Oligomeric peroxiredoxin-I is an essential intermediate for p53 to activate MST1 kinase and apoptosis.

    Science.gov (United States)

    Morinaka, A; Funato, Y; Uesugi, K; Miki, H

    2011-10-01

    Mammalian Ste20-like kinase-1 (MST1) kinase mediates H₂O₂-induced cell death by anticancer drugs such as cisplatin in a p53-dependent manner. However, the mechanism underlying MST1 activation by H₂O₂ remains unknown. Here we show that peroxiredoxin-I (PRX-I) is an essential intermediate in H₂O₂-induced MST1 activation and cisplatin-induced cell death through p53. Cell stimulation with H₂O₂ resulted in PRX-I oxidation to form homo-oligomers and interaction with MST1, leading to MST1 autophosphorylation and augmentation of kinase activity. In addition, RNA interference knockdown experiments indicated that endogenous PRX-I is required for H₂O₂-induced MST1 activation. Live-cell imaging showed H₂O₂ generation by cisplatin treatment, which likewise caused PRX-I oligomer formation, MST1 activation and cell death. Cisplatin-induced PRX-I oligomer formation was not observed in embryonic fibroblasts obtained from p53-knockout mice, confirming the importance of p53. Indeed, ectopic expression of p53 induced PRX-I oligomer formation and cell death, both of which were cancelled by the antioxidant NAC. Moreover, we succeeded in reconstituting H₂O₂-induced MST1 activation in vitro, using purified PRX-I and MST1 proteins. Collectively, our results show a novel PRX-I function to cause cell death in response to high levels of oxidative stress by activating MST1, which underlies the p53-dependent cytotoxicity caused by anticancer agents.

  13. Em busca da visibilidade: apropriações e produções de audiovisuais pelo MST * Searching for visibility: appropriations and audiovisual producions by MST

    Directory of Open Access Journals (Sweden)

    FERNANDO PERLI

    2013-12-01

    Full Text Available Resumo: A formação do Movimento dos Trabalhadores Rurais Sem Terra (MST marcou-se pelo envolvimento de entidades civis e religiosas na produção de diversos meios de comunicação. Dentre a variedade, os audiovisuais tornaram-se instrumentos cada vez mais recorrentes para a capacitação de quadros e a visibilidade social do MST. Na década de 1990, as coberturas dadas pela mídia e a ampliação dos mecanismos de divulgação do movimento social contribuíram para o desenvolvimento de projetos que incentivaram a produção de vídeos-documentários pelos sem-terra. O presente artigo suscita a análise das apropriações e produções de audiovisuais na organização do MST, considerando o sentido político do reconhecimento de audiovisuais para a divulgação do movimento social e o debate sobre o lugar ocupado por diferentes mecanismos de difusão de representações na luta pela reforma agrária.Palavras-chave: Audiovisuais – Movimentos sociais – Movimento dos Trabalhadores Rurais Sem-Terra. Abstract: The formation of the Landless Rural Workers Movement (MST was marked by the participation of civil and religious authorities in the production of various media. Among them, the audiovisual production became an increasingly recurrent instrument to train its cadre and to enable social visibility to the organization. In the 1990s, the media coverage and the expansion of dissemination mechanisms have contributed to the development of projects that stimulated the production of documentaries by the landless rural workers. This paper raises the analysis of appropriations and audiovisual productions within MST, considering the political sense of acknowledging the audiovisual as a means to disseminate the social movement as well as the debate on the place occupied by different diffusion mechanisms of representations in the struggle for agrarian reform.Keywords: Audiovisual – Social movement – Landless Rural Workers Movement.

  14. IL-6RFP can protect liver function in the model of mice infected with Schistosoma japonicum%IL-6RFP 在小鼠血吸虫感染中对肝细胞的保护作用

    Institute of Scientific and Technical Information of China (English)

    平春光; 程海燕; 高闻达; 计永胜; 刘淼; 任翠平; 邵延靖; 华梦晴; 沈际佳

    2015-01-01

    目的:探讨鼠白介素6受体融合蛋白(mIL-6RFP)在BALB/c 小鼠日本血吸虫感染模型中对肝细胞的保护作用。方法通过亲和层析方法获得可阻断白介素-6(IL-6)的mIL-6RFP,经人类肝癌细胞(HepG2)IL-6刺激及阻断实验检测纤维蛋白原(fibrinogen,FGG)和结合珠蛋白(haptoglobin, HP)的 mRNA 表达变化,体外验证重组蛋白 mIL-6RFP 的生物活性。建立 BALB/c 小鼠日本血吸虫感染模型,于感染第24天起经尾静脉注射 mIL-6RFP,隔天注射每次100μg/只,感染第42天剖杀小鼠。HE 染色法检测小鼠肝脏肉芽肿面积,荧光定量 PCR 法检测小鼠肝组织 IL-1β、IL-13、肿瘤坏死因子-α(TNF-α)和趋化因子1(CXCL1)的 mRNA 表达,连续监测法检测小鼠肝功能指标谷丙转氨酶(ALT)和谷草转氨酶(AST)。结果 HepG2细胞实验显示 mIL-6RFP 可降低IL-6对该细胞的刺激作用,显著下调 FGG 和 HP 的表达(P<0.05)。在小鼠感染模型中阻断 IL-6后,肝功能指标 ALT和 AST 与溶剂对照组相比均显著下降(P <0.05),肉芽肿病变无显著性差异。结论在 BALB/c 小鼠日本血吸虫感染模型中 mIL-6RFP 可明显改善肝细胞功能,但对肉芽肿形成未见明显作用。%Objective To explore the role of mouse interleukin-6 receptor fusion protein (mIL-6RFP)for the pro-tection of liver cells in the model of BALB /c mice infected with Schistosoma japonicum.Methods Nickel ion col-umn was used to obtain purified recombinant mIL-6RFP protein.In vitro,HepG2 cells were used to verify the bio-logical activity of mIL-6RFP.BALB /c mice were administrated by tail vein injection with 100 μg of mIL-6RFP or equal volume solvent,at 24,26,28,30,32,34,36,38,and 40 days after infection.Mice were sacrificed 48 h after the last injection.The granuloma size was measured in the mouse liver by HE staining.Interleukin-1β(IL-1β),interleukin-13 (IL-13 ),tumor

  15. '"My Land, Your Social Transformation": Conflicts within the Landless People Movement (MST), Rio De Janeiro, Brazil

    Science.gov (United States)

    Caldeira, Rute

    2008-01-01

    The Brazilian "Movimento dos Trabalhadores Rurais Sem-Terra" (MST) is one of the best-known and most prominent rural social movements. The unequal distribution of land in Brazil, and the neglect of this problem by successive Brazilian governments contributed greatly to the organisation of rural movements striving for the implementation…

  16. The Politics of the MST Autonomous Rural Communities, the State, and Electoral Politics

    NARCIS (Netherlands)

    Vergara Camus, Leandro

    Examination of the politicization of landless people in the encampments and settlements of the Movimento dos Trabalhadores Rurais Sem-Terra (Landless Rural Workers' Movement-MST) in Brazil suggests that the movement's success rest on the fact that it generates relatively autonomous rural communities

  17. The MST and the EZLN Struggle for Land : New Forms of Peasant Rebellions

    NARCIS (Netherlands)

    Vergara-camus, Leandro

    2009-01-01

    In this article, the author reviews some of the conclusions of the literature on peasant rebellions in the light of current land struggles of the Landless Rural Workers' Movement (MST) in Brazil and the Zapatista Army of National Liberation (EZLN) in Chiapas, Mexico. The author argues that conventio

  18. The Politics of the MST Autonomous Rural Communities, the State, and Electoral Politics

    NARCIS (Netherlands)

    Vergara-Camus, Leandro

    2009-01-01

    Examination of the politicization of landless people in the encampments and settlements of the Movimento dos Trabalhadores Rurais Sem-Terra (Landless Rural Workers' Movement-MST) in Brazil suggests that the movement's success rest on the fact that it generates relatively autonomous rural communities

  19. PHLPP1 regulates contact inhibition by dephosphorylating Mst1 at the inhibitory site

    Energy Technology Data Exchange (ETDEWEB)

    Jung, Sujin; Kang, Jeong Gu [Targeted Gene Regulation Research Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Deajeon (Korea, Republic of); Lee, Ju Hee [Targeted Gene Regulation Research Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Deajeon (Korea, Republic of); Department of Biomolecular Science, University of Science and Technology, 217 Gajeong-ro, Yuseong-gu, Daejeon (Korea, Republic of); Song, Kyoung Jin [Targeted Gene Regulation Research Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Deajeon (Korea, Republic of); Ko, Jeong-Heon [Targeted Gene Regulation Research Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Deajeon (Korea, Republic of); Department of Biomolecular Science, University of Science and Technology, 217 Gajeong-ro, Yuseong-gu, Daejeon (Korea, Republic of); Kim, Yong-Sam, E-mail: omsys1@kribb.re.kr [Targeted Gene Regulation Research Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Deajeon (Korea, Republic of); Department of Biomolecular Science, University of Science and Technology, 217 Gajeong-ro, Yuseong-gu, Daejeon (Korea, Republic of)

    2014-01-24

    Highlights: • PHLPP1 regulates contact inhibition by dephosphorylating Mst1 at Thr{sup 387}. • Overexpression of PHLPP1 sensitizes contact inhibition. • Tumor cells with suppressed PHLPP1 expression are refractory to apoptosis and highly proliferative. • Loss or down-regulation of PHLPP1 may drive tumor development and progression. - Abstract: Contact inhibition has been largely elusive despite that a loss of contact inhibition is a critical event for cancer development and progression. Here, we report that PHLPP1 is a binding protein for Mst1 and it modulates the Hippo pathway by dephosphorylating Mst1 at the inhibitory Thr{sup 387} of Mst1. Yap1 was localized predominantly in the nucleus but marginally in the cytoplasm in HeLa cells under sparse conditions, whereas the functional protein was more directed to sequestration in the cytoplasm under dense environments. Furthermore, loss of PHLPP1 resulted in a failure of the apoptotic control. It is interesting that down-regulated expression of PHLPP1 appears to mimic the loss of contact inhibition, a hallmark of cancer.

  20. Reframing the Public in Public Education: The Landless Workers Movement (MST) and Adult Education in Brazil

    Science.gov (United States)

    Thapliyal, Nisha

    2013-01-01

    Education for rural Brazilians has historically been dominated by two imperatives: human capital and political patronage. For the last four decades, the Landless Workers Movement (MST) have maintained a struggle to democratise public education and democracy itself. In this article, I make a situated analysis of the educational politics of the MST…

  1. The MST and the EZLN Struggle for Land : New Forms of Peasant Rebellions

    NARCIS (Netherlands)

    Vergara-camus, Leandro

    2009-01-01

    In this article, the author reviews some of the conclusions of the literature on peasant rebellions in the light of current land struggles of the Landless Rural Workers' Movement (MST) in Brazil and the Zapatista Army of National Liberation (EZLN) in Chiapas, Mexico. The author argues that conventio

  2. X-ray analysis of electron Bernstein wave heating in MST

    Science.gov (United States)

    Seltzman, A. H.; Anderson, J. K.; DuBois, A. M.; Almagri, A.; Forest, C. B.

    2016-11-01

    A pulse height analyzing x-ray tomography system has been developed to detect x-rays from electron Bernstein wave heated electrons in the Madison symmetric torus reversed field pinch (RFP). Cadmium zinc telluride detectors are arranged in a parallel beam array with two orthogonal multi-chord detectors that may be used for tomography. In addition a repositionable 16 channel fan beam camera with a 55° field of view is used to augment data collected with the Hard X-ray array. The chord integrated signals identify target emission from RF heated electrons striking a limiter located 12° toroidally away from the RF injection port. This provides information on heated electron spectrum, transport, and diffusion. RF induced x-ray emission from absorption on harmonic electron cyclotron resonances in low current (<250 kA) RFP discharges has been observed.

  3. X-ray analysis of electron Bernstein wave heating in MST

    Energy Technology Data Exchange (ETDEWEB)

    Seltzman, A. H., E-mail: seltzman@wisc.edu; Anderson, J. K.; DuBois, A. M.; Almagri, A.; Forest, C. B. [Department of Physics, University of Wisconsin, Madison, Wisconsin 53706 (United States)

    2016-11-15

    A pulse height analyzing x-ray tomography system has been developed to detect x-rays from electron Bernstein wave heated electrons in the Madison symmetric torus reversed field pinch (RFP). Cadmium zinc telluride detectors are arranged in a parallel beam array with two orthogonal multi-chord detectors that may be used for tomography. In addition a repositionable 16 channel fan beam camera with a 55° field of view is used to augment data collected with the Hard X-ray array. The chord integrated signals identify target emission from RF heated electrons striking a limiter located 12° toroidally away from the RF injection port. This provides information on heated electron spectrum, transport, and diffusion. RF induced x-ray emission from absorption on harmonic electron cyclotron resonances in low current (<250 kA) RFP discharges has been observed.

  4. Evaluation of no-MST operations in the SRS ARP for Hanford LAWPS

    Energy Technology Data Exchange (ETDEWEB)

    Herman, D. [Savannah River Site (SRS), Aiken, SC (United States)

    2016-11-14

    The Savannah River Site (SRS) Actinide Removal Process has been processing salt waste since 2008. This process includes a filtration step in the 512-S facility. Initial operations included the addition, or strike, of monosodium titanate (MST) to remove soluble actinides and strontium. The added MST and any entrained sludge solids were then separated from the supernate by cross flow filtration. During this time, the filter operations have, on many occasions, been the bottleneck process limiting the rate of salt processing. Recently, 512-S- has started operations utilizing “No-MST” where the MST actinide removal strike was not performed and the supernate was simply pre-filtered prior to Cs removal processing. Direct filtration of decanted tank supernate, as demonstrated in 512-S, is the proposed method of operation for the Hanford Low Activity Waste Pretreatment System (LAWPS) facility. Processing decanted supernate without MST solids has been demonstrated for cross flow filtration to provide a significant improvement in production with the SRS Salt Batches 8 and 9 feed chemistries. The average filtration rate for the first 512-S batch processing cycle using No-MST has increased filtrate production by over 35% of the historical average. The increase was sustained for more than double the amount of filtrate batches processed before cleaning of the filter was necessary. While there are differences in the design of the 512-S and Hanford filter systems, the 512-S system should provide a reasonable indication of LAWPS filter performance with similar feed properties. Based on the data from the 512-S facility and with favorable feed properties, the LAWPS filter, as currently sized at over twice the size of the 512-S filter (532 square feet filtration area versus 235 square feet), has the potential to provide sustained filtrate production at the upper range of the planned LAWPS production rate of 17 gpm.

  5. Migration stimulation factor (MStF), from murine B cells, constitutively produced by a T-B hybridoma.

    Science.gov (United States)

    Gauthier-Rahman, S; el-Gharbi, N; Bouvet, J P; Goodhart, M; Decreusefond, C; Couderc, J

    1992-10-01

    Hybridomas were established between murine spleen B cells and the thymoma cell line BW5147, to purify the migration stimulation factor (MStF), a molecule likely involved in immunosuppression. The parental B cells were from Lo/PHA mice previously shown to produce high levels of MStF after immunization by appropriate (tolerogenic) doses of ovalbumin. Among the positive clones, B9 was selected, since it produced high levels of MStF constitutively, and no immunoglobulin. This clone was shown to contain the genome of the B-cell fusion partner, since one of its L chain genes had undergone a VK-JK rearrangement. Isolation of MStF by size-exclusion chromatography showed 2 major peaks of activity, one of which eluted in a 20-kDa, almost protein-free fraction. This elution is unlikely to correspond to the true molecular mass, since MStF was found not to be a protein. Indeed, MStF was TCA-soluble, thermoresistant, highly hydrophobic and protease-resistant, but activity was abolished by neuraminidase digestion. The possibility of its being a small molecule transported by a protein carrier was also ruled out. These results suggest that MStF is a complex molecule containing both sialic residues and a lipid moiety. Experiments are planned to further investigate the chemical structure of this unusual B-cell factor.

  6. Jornadas de trabalho de mulheres e homens em um assentamento do MST Workdays of men and women in a collective settlement of the landless movement (MST

    Directory of Open Access Journals (Sweden)

    Giovana Ilka Jacinto Salvaro

    2004-04-01

    Full Text Available Este texto busca discutir a divisão do trabalho em um assentamento coletivo do Movimento dos Trabalhadores Rurais Sem Terra (MST, em Santa Catarina, marcado pela proposta de coletivização da terra e dos meios de produção. Nessa forma de organização, busca-se, seguindo as diretrizes estabelecidas pelo MST, a transformação igualitária e solidária da sociedade, incluindo a construção de novas relações de gênero. O que se observou é que, no cotidiano, mulheres e homens, sujeitos históricos e culturais, apropriam-se desses discursos de gênero, ao mesmo tempo que buscam lidar com as contradições que se apresentam. Entre estas, colocam-se as diferentes jornadas de trabalho que, apoiadas em padrões relacionais fixos, determinam oito horas diárias para os homens na produção e quatro para as mulheres, em função do trabalho doméstico e do cuidado das crianças.This text presents a discussion on the division of labor in a collective settlement of the Landless Movement (Movimento dos Trabalhadores Rurais Sem Terra - MST in Santa Catarina, characterized by the collectivization of land and of all means of production. Within this form of organization and according to the directives established by the MST, an equalitarian and solidarious transformation of society, including the construction of new genre relations, is sought for. What has been noted, throughout the daily routine, is that men and women alike, both historical and cultural subjects, appropriate this discourse at the same time in which they try to deal with upcoming contradictions. Within the latter, and based on fixed relational standards, different work hours are thus established. Men are determined to work on a daily eight hour basis in production whereas women, due to domestic work and child rearing, are granted a four hour schedule.

  7. Yeast Two Hybrid of MST1 and Its Association with Salvador%MST1的酵母双杂交及与Salvador体外结合的研究

    Institute of Scientific and Technical Information of China (English)

    张乐帅; 赵健

    2006-01-01

    利用酵母双杂交系统,以鼠MST1(Mammalian STE20-like 1)为诱饵蛋白,在鼠胚胎库中筛选到22个Salvador的片段.通过E.coli表达系统纯化了6个组氨酸(6His)融合的MST1和谷胱甘肽硫转移酶(GST)融合的Salvador,并做体外蛋白质结合实验,进一步证实了两蛋白质相互结合.通过体外激酶活性分析,发现MST1并不直接磷酸化Salvador,但是Salvador能够较强地抑制MST1对MBP(磷酸丁酯)的磷酸化,提示有可能Salvador通过影响MST1的激酶活性来参与了MST1介导的细胞凋亡途径.

  8. Indian MST radar: A remote sensing tool for studying long- and short- term cross tropopause transports

    Science.gov (United States)

    Das, Siddarth Shankar; Ratnam, Madineni Venkat; Suneeth, K. V.

    The stratospheric air is dry and ozone rich in nature, whereas the tropospheric air is enriched in humidity with high aerosol concentration. The tropopause is the stable layer which acts as a semi-permeable membrane between these two spheres (i.e. troposphere and stratosphere) and thus hinders the exchange of the minors constituents (e.g. water vapour and ozone) between these two spheres. This complex equilibrium region is known as the upper troposphere and lower stratosphere (UTLS) region. The major mixing process befalls over the tropical region, where the maximum convective systems occur through the tropical tropopause layer (TTL). Thus, it is necessary to understand the dynamical and chemical processes that occurring at UTLS region and quantative analysis of the mass exchange is essential. Ten years of data obtained from Indian MST radar located at a Tropical station Gadanki (13.5oN, 79.2oE) is used to study the long- and short (event) term cross tropopause transport. The prime mechanisms responsible for the radar backscattering echoes are isotropic/anisotropic turbulence fluctuations in the refractive index and Fresnel reflection/scattering due to sharp gradients in the radio refractive index. The inhomogeneties in the radio refractive index are contributed by both humidity and temperature below 8 km height, whereas above it, it is only contributed by the temperature gradients. When the dry stratospheric air penetrates into the troposphere, it will take some time to mix with the humid air of troposphere. Due to two different constituents of the air, there will be strong refractive index gradient, which will be reflected as enhanced radar backscattering echoes. The dry stratospheric air will slowly mix with the humid tropospheric air by means of small scale turbulence. Using this scattering mechanism behavior, the long-term and its seasonal characteristics, and the short-term event wise (convective disturbances) stratospheric air intrusion into the troposphere

  9. Globalización, tierra, resistencia y autonomía: el EZLN y el MST Globalization, land, resistance and autonomy: the EZLN and the MST

    Directory of Open Access Journals (Sweden)

    Leandro Vergara-Camus

    2011-09-01

    Full Text Available Este artículo presenta una comparación de la lucha por la tierra del EZLN en Chiapas y del MST en Brasil en el marco de la globalización. Se postula que las bases sociales de estos movimientos, desde su contexto específico, rechazan la lógica del mercado y adoptan estrategias de resistencia que privilegian la producción de subsistencia. Se postula por fin que la lucha por tener y mantener el acceso a la tierra produce un control territorial que genera un poder popular autónomo que desemboca en estrategias políticas distintas, resultado de las experiencias concretas que han tenido los movimientos con los partidos políticos y el Estado.This article compares the land struggle of the EZLN in Chiapas and the MST in Brazil within the context of globalization. The author argues that the membership of these movements, each within its own context, rejects the logic of the market and adopts resistance strategies that privilege production for subsistence. In addition, the author argues that in both cases the struggle to gain and maintain access to land gives these movements the ability to control territories, thus generating an autonomous popular power, which results in different political strategies that are the product of the concrete experiences that these movements have had with political parties and the state.

  10. Differential expression of MST4, STK25 and PDCD10 between benign prostatic hyperplasia and prostate cancer.

    Science.gov (United States)

    Zhang, Heyu; Ma, Xi; Peng, Saihui; Nan, Xu; Zhao, Hongshan

    2014-01-01

    Both benign prostatic hyperplasia (BPH) and prostate cancer (PC) are common diseases for men around the world. Both serine/threonine protein kinase MST4 (MST4) and serine/threonine kinase 25 (STK25) belong to the Ste20-like kinases and interact with programmed cell death 10 (PDCD10) which is closely linked to cancer diseases. To clarify the roles of MST4, STK25 and PDCD10 in prostate carcinogenesis, we examined MST4, STK25 and PDCD10 expression in tissue microarray blocks containing 110 cores of BPH and 160 cores of PC immunohistochemically and evaluated their correlation with clinicopathological findings. MST4 was not expressed in all the BPH cases and expressed in 38.7% of PC cases (P prostate cancer cases, PDCD10 staining was weak in 95 (59.4%) and strong in 65 (40.6%) (P prostatic hyperplasia cases (P prostate cancer (only 26.5% of these cases staining strongly; P prostate cancer and may play roles in prostate tumorigenesis. MST4 may be a helpful marker for identifying prostate cancer.

  11. Inherited MST1 deficiency underlies susceptibility to EV-HPV infections.

    Directory of Open Access Journals (Sweden)

    Amandine Crequer

    Full Text Available Epidermodysplasia verruciformis (EV is characterized by persistent cutaneous lesions caused by a specific group of related human papillomavirus genotypes (EV-HPVs in otherwise healthy individuals. Autosomal recessive (AR EVER1 and EVER2 deficiencies account for two thirds of known cases of EV. AR RHOH deficiency has recently been described in two siblings with EV-HPV infections as well as other infectious and tumoral manifestations. We report here the whole-exome based discovery of AR MST1 deficiency in a 19-year-old patient with a T-cell deficiency associated with EV-HPV, bacterial and fungal infections. MST1 deficiency has recently been described in seven patients from three unrelated kindreds with profound T-cell deficiency and various viral and bacterial infections. The patient was also homozygous for a rare ERCC3 variation. Our findings broaden the clinical range of infections seen in MST1 deficiency and provide a new genetic etiology of susceptibility to EV-HPV infections. Together with the recent discovery of RHOH deficiency, they suggest that T cells are involved in the control of EV-HPVs, at least in some individuals.

  12. Suppression of runaway electrons with a resonant magnetic perturbation in MST tokamak plasmas

    Science.gov (United States)

    Munaretto, Stefano; Chapman, B. E.; Almagri, A. F.; Cornille, B. S.; Dubois, A. M.; Goetz, J. A.; McCollam, K. J.; Sovinec, C. R.

    2016-10-01

    Runaway electrons generated in MST tokamak plasmas are now being probed with resonant magnetic perturbations (RMP's). An RMP with m =3 strongly suppresses the runaway electrons. Initial modeling of these plasmas with NIMROD shows the degradation of flux surfaces with an m =3 RMP, which may account for the runaway electron suppression. These MST tokamak plasmas have Bt =0.14 T, Ip =50kA, and q(a) =2.2, with a bulk electron density and temperature of 5x1017 m-3 and 150 eV. Runaway electrons are detected via x-ray emission. The RMP is produced by a poloidal array of 32 saddle coils at the narrow vertical insulated cut in MST's thick conducting shell. Each RMP has a single m but a broad n spectrum. A sufficiently strong m =3 RMP completely suppresses the runaway electrons, while a comparable m =1 RMP has little effect. The impact of the RMP's on the magnetic topology of these plasmas is being studied with the nonlinear MHD code, NIMROD. With an m =3 RMP, stochasticity is introduced in the outer third of the plasma. No such change is observed with the m =1 RMP. NIMROD also predicts regularly occurring sawtooth oscillations with a period comparable to MHD activity observed in the experiment. Work supported by USDOE.

  13. MST-BASED CLUSTERING TOPOLOGY CONTROL ALGORITHM FOR WIRELESS SENSOR NETWORKS

    Institute of Scientific and Technical Information of China (English)

    Cai Wenyu; Zhang Meiyan

    2010-01-01

    In this paper,we propose a novel clustering topology control algorithm named Minimum Spanning Tree (MST)-based Clustering Topology Control (MCTC) for Wireless Sensor Networks (WSNs),which uses a hybrid approach to adjust sensor nodes' transmission power in two-tiered hierarchical WSNs. MCTC algorithm employs a one-hop Maximum Energy & Minimum Distance (MEMD) clustering algorithm to decide clustering status. Each cluster exchanges information between its own Cluster Members (CMs) locally and then deliveries information to the Cluster Head (CH). Moreover,CHs exchange information between CH and CH and afterwards transmits aggregated information to the base station finally. The intra-cluster topology control scheme uses MST to decide CMs' transmission radius,similarly,the inter-cluster topology control scheme applies MST to decide CHs' transmission radius. Since the intra-cluster topology control is a full distributed approach and the inter-cluster topology control is a pure centralized approach performed by the base station,therefore,MCTC algorithm belongs to one kind of hybrid clustering topology control algorithms and can obtain scalability topology and strong connectivity guarantees simultaneously. As a result,the network topology will be reduced by MCTC algorithm so that network energy efficiency will be improved. The simulation results verify that MCTC outperforms traditional topology control schemes such as LMST,DRNG and MEMD at the aspects of average node's degree,average node's power radius and network lifetime,respectively.

  14. Educação do MST e crise do paradigma moderno de ciência

    Directory of Open Access Journals (Sweden)

    RUI GOMES DE MATTOS DE MESQUITA

    2014-01-01

    Full Text Available Hemos analizado el principio de alternancia en las prácticas pedagógicas del Movimento dos Trabalhadores Rurais Sem Terra (MST,adoptado en el Programa Nacional de la Reforma Agraria, a fin de identificar –asumiendo que dichas prácticas cuestionan el intento del pragmatismo de armonización entre individuo y sociedad– cómo este principio puede hacer que surjan sujetos “creativos”. La metodología empleada articula Maingueneau, Laclau y Mesquita. Hemos construido cuatro categorías (elevación de potencia; necesidad de relación con los medios instituidos; tensión con dichos medios; deseo ideológico de universalización e tres dimensiones analíticas (metodología; especialidad; finalidad. Con base en esta metodología analizamos dos documentos del MST (uno público y otro de circulación interna del movimiento, cuatro entrevistas (Militantes 1, 2 y 3 del MST y una profesora universitaria, así como artículos científicos de intelectuales orgánicos del Movimiento. Hemos concluido que conducir creativamente la experiencia significa romper con la noción de “acumulativo” en la formulación de principios.

  15. Improved SNR of MST RADAR Signals by Cosine Hyperbolic Window over Kaiser Window

    Directory of Open Access Journals (Sweden)

    Prof. B. Ramesh Reddy Dr. A. Subbarami Reddy Dr. P. Chandrashekar Reddy

    2012-02-01

    Full Text Available In this paper, the effect of window shape parameter “” in Cosine Hyperbolic Window Function on the SNR values of MST RADAR returns has been investigated. Six sets of multibeam observations of the lower atmosphere made by the Indian Mesosphere-Stratosphere-Troposphere (MST RADAR are used for the result analysis. Prior to the Fourier Transformation, the in-phase and quadrature components of the RADAR echo samples are weighted with the recently proposed new class of adjustable window based on the Cosine Hyperbolic Window Function. The effects of data weighting with the variation of the window shape parameter “” of the Cosine Hyperbolic Window Function is presented. It is observed that the increase of “” increases the SNR values and a good improvement is reported. Optimum value of the shape parameter “” for the Cosine Hyperbolic Window Function is suggested to analyze the MST RADAR signals. The results also shows that, the improvement of SNR of noisy data due to the effect of side lobe reduction and demands for the design of optimal windows.

  16. Effect of Mst1 overexpression on the growth of human hepatocellular carcinoma HepG2 cells and the sensitivity to cisplatin in vitro

    Institute of Scientific and Technical Information of China (English)

    Chuanming Xu; Chunju Liu; Wei Huang; Shuo Tu; Fusheng Wan

    2013-01-01

    Mammalian STE20-like kinase 1 (Mst1) is the mammalian homologue of Drosophila Hippo,a major inhibitor of cell proliferation in Drosophila.It ubiquitously encodes serine threonine kinase that belongs to the family of protein kinases related to yeast STE20,and is involved in cell proliferation,apoptosis,oncogenesis,and organ growth.Recent studies have shown that Mst1 has tumor-suppressor function,and the deletion or mutation of Mst1 is reported to be associated with tumorigenesis.To investigate the effect of overexpression of Mst1 on the growth of human liver cancer cell line HepG2 cells and the sensitivity to cisplatin in vitro,here we constructed recombinant eukaryotic expression vector pEGFP-N1-Mst1 containing Mst1 gene,and transiently transfected into HepG2 cells.The effects of Mst1 overexpression on the cell proliferation and apoptosis,the phosphorylation status of Yes-associated protein,and the mRNA transcript levels of connective tissue growth factor (CTGF),amphiregulin (AREG),and birc5 (Survivin) were determined.Results showed that overexpression of Mst1 inhibited cell proliferation,induced apoptosis of HepG2 cells,promoted YAP (Ser127) phosphorylation,and downregulated the mRNA expression of CTGF,AREG,and Survivin.We also investigated the relationship between the expression and cleavage of Mst1 and cisplatin-induced cell death.We found that Mst1 overexpression could induce cisplatin chemosensitivity,and cisplatin could promote the cleavage of Mst1 without affecting the expression of Mst1.Overall,our results indicated that Mst1 might be a promising anticancer target.

  17. 表达红荧光蛋白的重组痘苗病毒的包装与鉴定%Packing and identifcation of recombinant vaccinia virus expressing the RFP gene

    Institute of Scientific and Technical Information of China (English)

    沈百庆; 程乾; 罗砚曦; 阎辉倡; 郑骏年

    2015-01-01

    Objective To pack and identify the recombinant vaccinia virus ( VV-RFP) expressing red fluorescent protein ( RFP) gene.Methods The full-length sequence of RFP gene was PCR-amplified using plasmid pDsRed-express-C1 as the template.The RFP fragment was cloned into pCB vaccinia expression vector and the resulting recom-binant plasmid pCB-RFP was confirmed correct by restriction analysis and DNA sequencing.After 293 cells were trans-fected with the recombinant plasmid using liposome then infected with wild -type vaccinia virus, the VV-RFP was packed through homologous recombination and subsequently selected and screened by plaque-purified, taking advantage of RFP marker and gpt resistance.Results The recombinant vaccinia virus VV-RFP expressing RFP gene was con-structed successfully.Conclusion The packing of VV-RFP will provides a useful tool for the construction of recombi-nant vaccinia virus carrying therapeutic genes.%目的 包装并鉴定表达红荧光蛋白( RFP)基因的重组痘苗病毒( VV-RFP). 方法 以质粒pDsRed-express-C1为模板,PCR扩增出RFP基因,并克隆至痘苗病毒转移载体pCB中,经酶切分析和DNA测序鉴定正确,得到阳性重组质粒pCB-RFP. 将该重组质粒用脂质体转染293细胞后再用野生型痘苗病毒感染 ,通过同源重组获得重组痘苗病毒,利用RFP和gpt抗性标志筛选和噬斑纯化获得重组痘苗病毒VV-RFP. 结果 成功包装出表达RFP基因的重组痘苗病毒. 结论 包装的VV-RFP为进一步构建携带特定治疗基因的重组痘苗病毒奠定了基础.

  18. First mesospheric turbulence study using coordinated rocket and MST radar measurements over Indian low latitude region

    Energy Technology Data Exchange (ETDEWEB)

    Chandra, H.; Sinha, H.S.S.; Das, U.; Misra, R.N.; Das, S.R. [Physical Research Lab., Ahmedabad (India); Datta, J.; Chakravarty, S.C. [ISRO Headquarters, Bangalore (India); Patra, A.K.; Vekateswara Rao, N.; Narayana Rao, D. [National Atmospheric Research Lab., Tirupati (India)

    2008-07-01

    A campaign to study turbulence in the mesosphere, over low latitudes in India, using rocket-borne measurements and Indian MST radar, was conducted during July 2004. A rocket-borne Langmuir probe detected a spectrum of electron density irregularities, with scale sizes in the range of about 1 m to 1 km, in 67.5-78.0 km and 84-89 km altitude regions over a low latitude station Sriharikota (13.6 N, 80.2 E). A rocket-borne chaff experiment measured zonal and meridional winds about 30 min after the Langmuir probe flight. The MST radar located at Gadanki (13.5 N, 79.2 E), which is about 100 km west of Sriharikota, also detected the presence of a strong scattering layer in 73.5-77.5 km region from which radar echoes corresponding to 3 m irregularities were received. Based on the region of occurrence of irregularities, which was highly collisional, presence of significant shears in zonal and meridional components of wind measured by the chaff experiment, 10 min periodicity in zonal and meridional winds obtained by the MST radar and the nature of wave number spectra of the irregularities, it is suggested that the observed irregularities were produced through the neutral turbulence mechanism. The percentage amplitude of fluctuations across the entire scale size range showed that the strength of turbulence was stronger in the lower altitude regions and decreased with increasing altitude. It was also found that the amplitude of fluctuations was large in regions of steeper electron density gradients. MST radar observations showed that at smaller scales of turbulence such as 3 m, (a) the thickness of the turbulent layer was between 2 and 3 km and (b) and fine structures, with layer thicknesses of about a km or less were also embedded in these layers. Rocket also detected 3-m fluctuations, which were very strong (a few percent) in lower altitudes (67.5 to 71.0 km) and small but clearly well above the noise floor at higher altitudes. Rocket and radar results also point to the

  19. First mesospheric turbulence study using coordinated rocket and MST radar measurements over Indian low latitude region

    Directory of Open Access Journals (Sweden)

    H. Chandra

    2008-09-01

    Full Text Available A campaign to study turbulence in the mesosphere, over low latitudes in India, using rocket-borne measurements and Indian MST radar, was conducted during July 2004. A rocket-borne Langmuir probe detected a spectrum of electron density irregularities, with scale sizes in the range of about 1 m to 1 km, in 67.5–78.0 km and 84–89 km altitude regions over a low latitude station Sriharikota (13.6° N, 80.2° E. A rocket-borne chaff experiment measured zonal and meridional winds about 30 min after the Langmuir probe flight. The MST radar located at Gadanki (13.5° N, 79.2° E, which is about 100 km west of Sriharikota, also detected the presence of a strong scattering layer in 73.5–77.5 km region from which radar echoes corresponding to 3 m irregularities were received. Based on the region of occurrence of irregularities, which was highly collisional, presence of significant shears in zonal and meridional components of wind measured by the chaff experiment, 10 min periodicity in zonal and meridional winds obtained by the MST radar and the nature of wave number spectra of the irregularities, it is suggested that the observed irregularities were produced through the neutral turbulence mechanism. The percentage amplitude of fluctuations across the entire scale size range showed that the strength of turbulence was stronger in the lower altitude regions and decreased with increasing altitude. It was also found that the amplitude of fluctuations was large in regions of steeper electron density gradients. MST radar observations showed that at smaller scales of turbulence such as 3 m, (a the thickness of the turbulent layer was between 2 and 3 km and (b and fine structures, with layer thicknesses of about a km or less were also embedded in these layers. Rocket also detected 3-m fluctuations, which were very strong (a few percent in lower altitudes (67.5 to 71.0 km and small but clearly well above the noise floor at higher altitudes. Rocket and radar

  20. 23 CFR 636.401 - What types of information exchange may take place prior to the release of the RFP document?

    Science.gov (United States)

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false What types of information exchange may take place prior... What types of information exchange may take place prior to the release of the RFP document? Verbal or written information exchanges (such as in the first-phase of a two-phase selection procedure) must...

  1. MAPKs and Mst1/Caspase-3 pathways contribute to H2B phosphorylation during UVB-induced apoptosis

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Apoptosis is a highly coordinated or programmed cell suicide mechanism in eukaryotes.Histone modification is associated with nuclear events in apoptotic cells.Specifically H2B phosphorylation at serine 14 (Ser14) catalyzed by Mst1 kinase has been linked to chromatin condensation during apoptosis.We report that activation of MAPKs (ERK1/2,JNK1/2 and p38) together with Mst1 and caspase-3 is required for phosphorylation of H2B (Ser14) during ultraviolet B light (UVB)-induced apoptosis.UVB can trigger activation of MAPKs and induce H2B phosphorylation at Ser14 but not acetylation in a time-dependent manner.Inhibition of ERK1/2,JNK1/2 or p38 activity blocked H2B phosphorylation (Ser14).Furthermore,caspase-3 was activated by UVB to regulate Mst1 activity,which phosphorylates H2B at Ser14,leading to chromatin condensation.Full inhibition of caspase-3 activity reduced Mst1 activation and partially inhibited H2B phosphorylation (Ser14),but ERK1/2,JNK1/2 and p38 activities were not affected.Taken together,these data revealed that H2B phosphorylation is regulated by both MAPKs and caspase-3/Mst1 pathways during UVB-induced apoptosis.

  2. Shikonin regulates C-MYC and GLUT1 expression through the MST1-YAP1-TEAD1 axis.

    Science.gov (United States)

    Vališ, Karel; Talacko, Pavel; Grobárová, Valéria; Černý, Jan; Novák, Petr

    2016-12-10

    The general mechanism underlying the tumor suppressor activity of the Hippo signaling pathway remains unclear. In this study, we explore the molecular mechanisms connecting the Hippo signaling pathway with glucose metabolism. We have found that two key regulators of glycolysis, C-MYC and GLUT1, are targets of the Hippo signaling pathway in human leukemia cells. Our results revealed that activation of MST1 by the natural compound shikonin inhibited the expression of GLUT1 and C-MYC. Furthermore, RNAi experiments confirmed the regulation of GLUT1 and C-MYC expression via the MST1-YAP1-TEAD1 axis. Surprisingly, YAP1 was found to positively regulate C-MYC mRNA levels in complex with TEAD1, while it negatively regulates C-MYC levels in cooperation with MST1. Hence, YAP1 serves as a rheostat for C-MYC, which is regulated by MST1. In addition, depletion of MST1 stimulates lactate production, whereas the specific depletion of TEAD1 has an opposite effect. The inhibition of lactate production and cellular proliferation induced by shikonin also depends on the Hippo pathway activity. Finally, a bioinformatic analysis revealed conserved TEAD-binding motifs in the C-MYC and GLUT1 promoters providing another molecular data supporting our observations. In summary, regulation of glucose metabolism could serve as a new tumor suppressor mechanism orchestrated by the Hippo signaling pathway. Copyright © 2016 Elsevier Inc. All rights reserved.

  3. Studying small molecule-aptamer interactions using MicroScale Thermophoresis (MST).

    Science.gov (United States)

    Entzian, Clemens; Schubert, Thomas

    2016-03-15

    Aptamers are potent and versatile binding molecules recognizing various classes of target molecules. Even challenging targets such as small molecules can be identified and bound by aptamers. Studying the interaction between aptamers and drugs, antibiotics or metabolites in detail is however difficult due to the lack of sophisticated analysis methods. Basic binding parameters of these small molecule-aptamer interactions such as binding affinity, stoichiometry and thermodynamics are elaborately to access using the state of the art technologies. The innovative MicroScale Thermophoresis (MST) is a novel, rapid and precise method to characterize these small molecule-aptamer interactions in solution at microliter scale. The technology is based on the movement of molecules through temperature gradients, a physical effect referred to as thermophoresis. The thermophoretic movement of a molecule depends - besides on its size - on charge and hydration shell. Upon the interaction of a small molecule and an aptamer, at least one of these parameters is altered, leading to a change in the movement behavior, which can be used to quantify molecular interactions independent of the size of the target molecule. The MST offers free choice of buffers, even measurements in complex bioliquids are possible. The dynamic affinity range covers the pM to mM range and is therefore perfectly suited to analyze small molecule-aptamer interactions. This section describes a protocol how quantitative binding parameters for aptamer-small molecule interactions can be obtained by MST. This is demonstrated by mapping down the binding site of the well-known ATP aptamer DH25.42 to a specific region at the adenine of the ATP molecule.

  4. Application of the quantum mechanical IEF/PCM-MST hydrophobic descriptors to selectivity in ligand binding.

    Science.gov (United States)

    Ginex, Tiziana; Muñoz-Muriedas, Jordi; Herrero, Enric; Gibert, Enric; Cozzini, Pietro; Luque, F Javier

    2016-06-01

    We have recently reported the development and validation of quantum mechanical (QM)-based hydrophobic descriptors derived from the parametrized IEF/PCM-MST continuum solvation model for 3D-QSAR studies within the framework of the Hydrophobic Pharmacophore (HyPhar) method. In this study we explore the applicability of these descriptors to the analysis of selectivity fields. To this end, we have examined a series of 88 compounds with inhibitory activities against thrombin, trypsin and factor Xa, and the HyPhar results have been compared with 3D-QSAR models reported in the literature. The quantitative models obtained by combining the electrostatic and non-electrostatic components of the octanol/water partition coefficient yield results that compare well with the predictive potential of standard CoMFA and CoMSIA techniques. The results also highlight the potential of HyPhar descriptors to discriminate the selectivity of the compounds against thrombin, trypsin, and factor Xa. Moreover, the graphical representation of the hydrophobic maps provides a direct linkage with the pattern of interactions found in crystallographic structures. Overall, the results support the usefulness of the QM/MST-based hydrophobic descriptors as a complementary approach for disclosing structure-activity relationships in drug design and for gaining insight into the molecular determinants of ligand selectivity. Graphical Abstract Quantum Mechanical continuum solvation calculations performed with the IEF/PCM-MST method are used to derived atomic hydrophobic descriptors, which are then used to discriminate the selectivity of ligands against thrombin, trypsin and factor Xa. The descriptors provide complementary view to standard 3D-QSAR analysis, leading to a more comprehensive understanding of ligand recognition.

  5. Educação do MST e crise do paradigma moderno de ciência

    OpenAIRE

    RUI GOMES DE MATTOS DE MESQUITA; GISELE WANESSA DO NASCIMENTO

    2014-01-01

    Hemos analizado el principio de alternancia en las prácticas pedagógicas del Movimento dos Trabalhadores Rurais Sem Terra (MST),adoptado en el Programa Nacional de la Reforma Agraria, a fin de identificar –asumiendo que dichas prácticas cuestionan el intento del pragmatismo de armonización entre individuo y sociedad– cómo este principio puede hacer que surjan sujetos “creativos”. La metodología empleada articula Maingueneau, Laclau y Mesquita. Hemos construido cuatro categorías (elevación de ...

  6. Development of radio acoustic sounding system (RASS) with Gadanki MST radar – first results

    OpenAIRE

    T. V. Chandrasekhar Sarma; Narayana Rao, D.; Furumoto, J.; Tsuda, T.

    2008-01-01

    A high-power acoustic exciter was designed and developed for the Gadanki MST Radar to facilitate observations in the Radio Acoustic Sounding System (RASS) mode. Sweep range of acoustic signal frequencies was set to 94–125 Hz so as to satisfy Bragg matching condition for temperature range of −90°–40°C between surface and the tropopause (about 17 km). Raytracing of acoustic wave propagation was used to predict the antenna beam directions along which optimum RASS echoes could be ob...

  7. A brief history of the development of wind-profiling or MST radars

    Directory of Open Access Journals (Sweden)

    T. E. Van Zandt

    Full Text Available The history of the development of the wind-profiling or MST radar technique is reviewed from its inception in the late 1960s to the present. Extensions of the technique by the development of boundary-layer radars and the radio-acoustic sounding system (RASS technique to measure temperature are documented. Applications are described briefly, particularly practical applications to weather forecasting, with data from networks of radars, and scientific applications to the study of rapidly varying atmospheric phenomena such as gravity waves and turbulence.

    Key words: Meteorology and atmospheric dynamics (instruments and techniques · Radio science (remote sensing; instruments and techniques

  8. MS&T'13 Symposium Preview: Metal and Polymer Matrix Composites

    Science.gov (United States)

    Gupta, Nikhil; Paramsothy, Muralidharan

    2013-08-01

    The Metal and Polymer Matrix Composites symposium at Materials Science & Technology 2013 (MS&T'13) conference is planned to provide a platform to researchers working on various aspects of composite materials and capture the state of the art in this area. The dialogue among leading researchers is expected to provide insight into the future of this field and identify the future directions in terms of research, development, and applications of composite materials. In the 2 day program, the symposium includes 34 presentations, including 10 invited presentations. The contributions have come from 16 different countries including USA, Mexico, Switzerland, India, Egypt, and Singapore.

  9. Net and central locality: the MST in northwest of Paraná

    OpenAIRE

    Fabrini, João Edmilson; Universidade Estadual do Oeste do Paraná

    2011-01-01

    From Querência do Norte is articulated a whole of relations between settlements and camps in the northwest. This center is represented by of MST Secretariat and by Cooperativa de Comercialização e Reforma Agrária Avante Ltda (COANA), which are localized in Querência do Norte, it becomes a knot in the mediation between the settlements/camps and the State Secretariat (Curitiba) and National (São Paulo). Thus, Querência do Norte, While a central locality, articulates to a center of control an co...

  10. O caminho da terra: revisitando a história do MST no Pontal do Paranapanema - SP - desde uma ótica psicopolítica Los caminos de la tierra: reconstruyendo la memoria política del MST en el Pontal do Paranapanema - São Paulo Pathways to the land: recovering a political memory of MST - Brazil's landless workers' movement in western São Paulo, Brazil

    Directory of Open Access Journals (Sweden)

    Alessandro Soares da Silva

    2010-03-01

    Full Text Available No presente artigo pretendemos recuperar aspectos psicopolíticos da luta pela terra empreendida pelo MST no Pontal do Paranapanema - SP, conhecida região de conflitos fundiários. Este trabalho resulta de uma ampla revisão bibliográfica acerca do tema feita com o intuito de compreender os processos de construção deste movimento social e de resgatar mais do que os fatos históricos vividos por ele. Resgatar essa história contribui para a construção de uma memória coletiva destes homens e mulheres incógnitos; fazê-lo é conceder-lhes voz, visibilidade, é reconhecê-los e reconhecê-las como sujeitos políticos, mesmo que aqui apenas o façamos de maneira indireta a partir de nossos estudos e de outros que contribuíram para visibilizar a luta travada no campo nessa região paulista.En el presente artículo, pretendemos recuperar aspectos psicopolíticos de la lucha del MST por la tierra en la región del Pontal do Paranapanema - SP - Brasil. Esa es una conocida región de conflictos rurales en el Estado de São Paulo. Este trabajo resulta de una amplia revisión bibliográfica acerca del tema con el intuito de comprender los procesos de construcción de este movimiento social y de rescatar los fatos históricos vividos por él y contribuir para que también pues recatar a memoria colectiva de estos hombres y mujeres incógnitos/as; hacerlo es reconocerles como sujetos políticos, aunque de modo indirecto y partiendo de nuestros trabajos y de otros que contribuyen para visibilizar la lucha trabada en el Campo paulista.This paper unveils the political psychology of the shapping of a collective memory in the MST - Brazil's Landless Workers' Movement in the western corner of the state of Sao Paulo in Brazil, a region named Pontal do Paranapanema, known for its conflicts between landlords/large estate owners and landless rural workers. The paper results from an extensive review on the subject and highlights the underlying dinamics in the

  11. Emulating the visual receptive-field properties of MST neurons with a template model of heading estimation

    Science.gov (United States)

    Perrone, J. A.; Stone, L. S.

    1998-01-01

    We have proposed previously a computational neural-network model by which the complex patterns of retinal image motion generated during locomotion (optic flow) can be processed by specialized detectors acting as templates for specific instances of self-motion. The detectors in this template model respond to global optic flow by sampling image motion over a large portion of the visual field through networks of local motion sensors with properties similar to those of neurons found in the middle temporal (MT) area of primate extrastriate visual cortex. These detectors, arranged within cortical-like maps, were designed to extract self-translation (heading) and self-rotation, as well as the scene layout (relative distances) ahead of a moving observer. We then postulated that heading from optic flow is directly encoded by individual neurons acting as heading detectors within the medial superior temporal (MST) area. Others have questioned whether individual MST neurons can perform this function because some of their receptive-field properties seem inconsistent with this role. To resolve this issue, we systematically compared MST responses with those of detectors from two different configurations of the model under matched stimulus conditions. We found that the characteristic physiological properties of MST neurons can be explained by the template model. We conclude that MST neurons are well suited to support self-motion estimation via a direct encoding of heading and that the template model provides an explicit set of testable hypotheses that can guide future exploration of MST and adjacent areas within the superior temporal sulcus.

  12. Two-year follow-up of a randomized effectiveness trial evaluating MST for juveniles who sexually offend.

    Science.gov (United States)

    Letourneau, Elizabeth J; Henggeler, Scott W; McCart, Michael R; Borduin, Charles M; Schewe, Paul A; Armstrong, Kevin S

    2013-12-01

    Building on prior efficacy trials (i.e., university-based, graduate students as therapists), the primary purpose of this study was to determine whether favorable 12-month outcomes, obtained in a randomized effectiveness trial (i.e., implemented by practitioners in a community mental health center) of multisystemic therapy (MST) with juveniles who had sexually offended (JSO), were sustained through a second year of follow-up. JSO (n = 124 male youth) and their families were randomly assigned to MST, which was family based and delivered by community-based practitioners, or to treatment as usual (TAU), which was primarily group-based cognitive-behavioral interventions delivered by professionals within the juvenile justice system. Youth averaged 14.7 years of age (SD = 1.7) at referral, were primarily African American (54%), and 30% were Hispanic. All youth had been diverted or adjudicated for a sexual offense. Analyses examined whether MST effects reported previously at 1-year follow-up for problem sexual behaviors, delinquency, substance use, and out-of-home placement were sustained through a second year of follow-up. In addition, arrest records were examined from baseline through 2-year follow-up. During the second year of follow-up, MST treatment effects were sustained for 3 of 4 measures of youth problem sexual behavior, self-reported delinquency, and out-of-home placements. The base rate for sexual offense rearrests was too low to conduct statistical analyses, and a between-groups difference did not emerge for other criminal arrests. For the most part, the 2-year follow-up findings from this effectiveness study are consistent with favorable MST long-term results with JSO in efficacy research. In contrast with many MST trials, however, decreases in rearrests were not observed.

  13. Development of radio acoustic sounding system (RASS with Gadanki MST radar – first results

    Directory of Open Access Journals (Sweden)

    T. Tsuda

    2008-09-01

    Full Text Available A high-power acoustic exciter was designed and developed for the Gadanki MST Radar to facilitate observations in the Radio Acoustic Sounding System (RASS mode. Sweep range of acoustic signal frequencies was set to 94–125 Hz so as to satisfy Bragg matching condition for temperature range of −90°–40°C between surface and the tropopause (about 17 km. Raytracing of acoustic wave propagation was used to predict the antenna beam directions along which optimum RASS echoes could be obtained. During the RASS observation period of about 18 h on 23–24 July 2006 height profiles of atmospheric virtual temperature were obtained between 1.5 km and 10 km and occasionally up to 14 km. In comparison with the three simultaneous radiosonde launches, RASS derived temperature profiles had the r.m.s. discrepancy of about 1 K, although deviation of the RASS results sometimes appeared when the radial wind velocity was not fully available for the correction of apparent sound speed. This study has successfully demonstrated capability of the RASS application with the Gadanki MST radar, which will be used for continuous monitoring of the temperature profiles in the troposphere and lower stratosphere region in the tropics.

  14. First absolutely calibrated on-axis ion flow measurements in MST

    Science.gov (United States)

    Schott, B.; Baltzer, M.; Craig, D.; den Hartog, D. J.; Nishizawa, T.; Nornberg, M. D.

    2016-10-01

    Improvements in absolute calibration techniques allow for the first direct measurements of the flow profile in the core of MST. We use both active charge exchange recombination spectroscopy and passive emission near 343 nm to measure ion temperature and flow. It is generally assumed that O VI is the brightest passive emission source. However, we show that there are cases, such as high temperature, pulsed poloidal current drive (PPCD) plasmas where the passive emission is dominated by C VI. Differences in the fine structure for O VI and C VI result in a systematic velocity error of about 12 km/s if the wrong model is assumed. Active measurements, however, are relatively insensitive to background model choice. The dominant source of error in active velocity measurements remains the systematic errors in calibration. The first absolutely calibrated, localized toroidal velocity measurements were obtained using an updated calibration technique. During PPCD, the on-axis ion flow is up to 40 km/s larger than both the n = 6 mode velocity and the line-averaged ion velocity. These measurements provide the first direct look at the flow profile in the core of MST. This work has been supported by the US DOE and the Wheaton College summer research program.

  15. MST Fitness Index and implicit data narratives: A comparative test on alternative unsupervised algorithms

    Science.gov (United States)

    Buscema, Massimo; Sacco, Pier Luigi

    2016-11-01

    In this paper, we introduce a new methodology for the evaluation of alternative algorithms in capturing the deep statistical structure of datasets of different types and nature, called MST Fitness, and based on the notion of Minimum Spanning Tree (MST). We test this methodology on six different databases, some of which artificial and widely used in similar experimentations, and some related to real world phenomena. Our test set consists of eight different algorithms, including some widely known and used, such as Principal Component Analysis, Linear Correlation, or Euclidean Distance. We moreover consider more sophisticated Artificial Neural Network based algorithms, such as the Self-Organizing Map (SOM) and a relatively new algorithm called Auto-Contractive Map (AutoCM). We find that, for our benchmark of datasets, AutoCM performs consistently better than all other algorithms for all of the datasets, and that its global performance is superior to that of the others of several orders of magnitude. It is to be checked in future research if AutoCM can be considered a truly general-purpose algorithm for the analysis of heterogeneous categories of datasets.

  16. The MST of Symmetric Disk Graphs (in Arbitrary Metrics) is Light

    CERN Document Server

    Solomon, Shay

    2011-01-01

    Consider an n-point metric M = (V,delta), and a transmission range assignment r: V \\rightarrow \\mathbb R^+ that maps each point v in V to the disk of radius r(v) around it. The {symmetric disk graph} (henceforth, SDG) that corresponds to M and r is the undirected graph over V whose edge set includes an edge (u,v) if both r(u) and r(v) are no smaller than delta(u,v). SDGs are often used to model wireless communication networks. Abu-Affash, Aschner, Carmi and Katz (SWAT 2010, \\cite{AACK10}) showed that for any {2-dimensional Euclidean} n-point metric M, the weight of the MST of every {connected} SDG for M is O(log n) w(MST(M)), and that this bound is tight. However, the upper bound proof of \\cite{AACK10} relies heavily on basic geometric properties of 2-dimensional Euclidean metrics, and does not extend to higher dimensions. A natural question that arises is whether this surprising upper bound of \\cite{AACK10} can be generalized for wider families of metrics, such as 3-dimensional Euclidean metrics. In this pap...

  17. Prediction Models for Plutonium, Strontium, Uranium and Neptunium Loading onto Monosodium Titanate (MST)

    Energy Technology Data Exchange (ETDEWEB)

    Fondeur, F. F.; Hobbs, D. T.; Barnes, M. J.; Peters, T. B.; Fink, S. D.

    2005-07-11

    The DA isotherm parameters for U, Pu, Sr and Np have been updated to include additional data obtained since the original derivation. The DA isotherms were modified to include a kinetic function derived by Rahn to describe sorbate loading from the beginning of sorption up to equilibrium. The final functions describe both kinetic and thermodynamic sorption. We selected the Rahn function to describe radionuclide sorption because it originates from diffusion and absorption controlled sorption. An investigation of the thermal behavior of radionuclide sorption on MST as shown by this data revealed the sorption process is diffusion (or transport) controlled (in solution). Transport in solution can in theory be accelerated by vigorous mixing but the range of available mixing speed in the facility design will probably not be sufficient to markedly increase radionuclide sorption rate on MST from diffusion-controlled sorption. The laboratory studies included mixing energies hydraulically-scaled to match those of the Actinide Removal Process and these likely approximate the range of energies available in the Salt Waste Processing Facility.

  18. Os sentidos na mídia: o MST em dois jornais diários The meanings in the media: the MST in two daily periodicals

    Directory of Open Access Journals (Sweden)

    Alexandre Bonetti Lima

    2006-12-01

    Full Text Available Esta pesquisa objetiva participar da discussão sobre a mídia impressa a partir dos discursos sobre o MST do Pontal do Paranapanema em dois jornais diários. Ela situa-se no debate entre aquelas para as quais a mídia é um aparelho ideológico de dominação, e aquelas para as quais sua polissemia inevitável pode exercer participação construtiva numa sociedade de conflitos, mesmo sem intenção. A referência à Psicologia Social Construcionista nos localiza no segundo conjunto de reflexões, possibilitando contemplar o jornal diário como um mosaico no qual presentificam-se múltiplas dialogias e produções de sentidos acerca dos temas noticiados, configurando-o, simultaneamente, como: veículo de transmissão de diversas vozes, oriundas de diversos lugares e com diversos posicionamentos sobre o que noticia; como ator social com voz e posicionamentos próprios sobre o mesmo; como lugar de diálogo com os leitores, co-autores ativos dos sentidos e histórias contadas. Mostra-se, portanto, como espaço de expressão da dinâmica das lutas hegemônicas de um tempo e lugar.This research aims to contribute to the discussion about printed media, studying the discourses of two daily newspapers concerning the MST - Movimento Sem Terra (landless rural worker's movement from Pontal do Paranapanema. This work places itself in the debate between those positions that understand media as an ideological device of domination and those for whom the inevitable multiplicity of meanings from media may exert a constructive participation in a conflictive society, even though not intentionally. References to constructivist social psychology places our position in the second group of considerations and sees the daily newspaper as a mosaic in which multiple dialogues and meaning productions about the same theme coexist. This configures the newspaper in three simultaneous ways: as a means of conveying multiple voices, coming from different places and with

  19. Comprehensive treatment for co-occurring child maltreatment and parental substance abuse: outcomes from a 24-month pilot study of the MST-Building Stronger Families program.

    Science.gov (United States)

    Schaeffer, Cindy M; Swenson, Cynthia Cupit; Tuerk, Elena Hontoria; Henggeler, Scott W

    2013-08-01

    This manuscript presents outcomes from a pilot study of Multisystemic Therapy-Building Stronger Families (MST-BSF), an integrated treatment model for the co-occurring problem of parental substance abuse and child maltreatment among families involved in the child welfare system. Participants were 25 mother-youth dyads who participated in MST-BSF and an additional 18 families with similar demographic and case characteristics who received Comprehensive Community Treatment (CCT). At post-treatment, mothers who received MST-BSF showed significant reductions in alcohol use, drug use, and depressive symptoms; they also significantly reduced their use of psychological aggression with the youth. Youth reported significantly fewer anxiety symptoms following MST-BSF treatment. Relative to families who received CCT, mothers who received MST-BSF were three times less likely to have another substantiated incident of maltreatment over a follow-up period of 24 months post-referral. The overall number of substantiated reabuse incidents in this time frame also was significantly lower among MST-BSF families, and youth who received MST-BSF spent significantly fewer days in out-of-home placements than did their CCT counterparts. These promising preliminary outcomes support the viability of a more rigorous (i.e., randomized) evaluation of the MST-BSF model.

  20. Pengaruh mimosa pada penyamakan kulit jaket domba samak nabati menggunakan sistem C-RFP, ditinjau dari sifat organoleptis, fisis, dan morfologi kulit

    Directory of Open Access Journals (Sweden)

    Sri Sutyasmi

    2016-06-01

    Full Text Available The purpose of this study was to determine the effect of the use of mimosa, to manufacture environmentally friendly leather for jacket by using the C-RFP system (C=Conditioning, R=Rapid, F=Fass (drum, P=Powder, on physical, organoleptic, and morphology properties of leather. It was also to obtain a formula for vegetable tanning with C-RFP system. Pickled skins are conditioned (pre-tanning using Sodotan TSN and Sodotan APR, and then Sodotan TSN was chosen due to it meets the requirements of SNI leather for jacket (SNI 4593:2011. Then, pickled skins were tanned with mimosa and applied C-RFP system or rapid tanning without water added. Mimosa, used in this research, were 15%, 20%, and 25% and fatliquor were 12.5%, 15%, and 17.5%. Furthermore, The leather were finished into an environmentally friendly leather for jackets, and then tested for physical and organoleptic properties based on the SNI 4593:2011 as well as leather morphology (SEM. The physical test result shows that for mimosa 15%, 20% and 25%, and for fatliquor 15% and 17.5% are fullfill the SNI.

  1. Effects of peripherally administered cholecystokinin-8 and secretin on feeding/drinking and oxytocin-mRFP1 fluorescence in transgenic rats.

    Science.gov (United States)

    Motojima, Yasuhito; Kawasaki, Makoto; Matsuura, Takanori; Saito, Reiko; Yoshimura, Mitsuhiro; Hashimoto, Hirofumi; Ueno, Hiromichi; Maruyama, Takashi; Suzuki, Hitoshi; Ohnishi, Hideo; Sakai, Akinori; Ueta, Yoichi

    2016-08-01

    Peripheral administration of cholecystokinin (CCK)-8 or secretin activates oxytocin (OXT)-secreting neurons in the hypothalamus. Although OXT is involved in the regulation of feeding behavior, detailed mechanism remains unclear. In the present study, we examined the central OXTergic pathways after intraperitoneally (i.p.) administration of CCK-8 and secretin using male OXT-monomeric red fluorescent protein 1 (mRFP1) transgenic rats and male Wistar rats. I.p. administration of CCK-8 (50μg/kg) and secretin (100μg/kg) decreased food intake in these rats. While i.p. administration of CCK-8 decreased water intake, i.p. administration of secretin increased water intake. Immunohistochemical study revealed that Fos-Like-Immunoreactive cells were observed abundantly in the brainstem and in the OXT neurons in the dorsal division of the parvocellular paraventricular nucleus (dpPVN). We could observe marked increase of mRFP1 fluorescence, as an indicator for OXT, in the dpPVN and mRFP1-positive granules in axon terminals of the dpPVN OXT neurons in the nucleus tractus solitarius (NTS) after i.p. administration of CCK-8 and secretin. These results provide us the evidence that, at least in part, i.p. administration of CCK-8 or secretin might be involved in the regulation of feeding/drinking via a OXTergic pathway from the dpPVN to the NTS.

  2. In vivo near-infrared fluorescence imaging of Leishmania amazonensis expressing infrared fluorescence protein (iRFP) for real-time monitoring of cutaneous leishmaniasis in mice.

    Science.gov (United States)

    Oliveira, Janaina Correia; da Silva, Aline Caroline; Oliveira, Renato Antonio Dos Santos; Pereira, Valéria Rêgo Alves; Gil, Laura Helena Vega Gonzales

    2016-11-01

    The use of Leishmania amazonensis-infected BALB/c mice is an important model for the study of experimental cutaneous leishmaniasis. Here we report the development of a non-invasive method to directly evaluate and measure parasite burden during the course of the infection, based on the near-infrared fluorescence detection of a recombinant L. amazonensis strain. So, we generated a L. amazonensis strain that stably expresses the near-infrared protein (iRFP) gene and compared the maintenance of its vitro and in vivo characteristics, such as fitness, pathogenicity and fluorescence emission. After that, we followed the disease development, as well as the parasite burden in BALB/c mice footpads infected with L. amazonensis-iRFP, by using an in vivo near-infrared fluorescence scanner. In vitro results showed a linear correlation between the fluorescence emission and the number of parasites. The in vivo study showed that the use of iRFP-transfected L. amazonensis enables the monitoring of parasite burden by measuring fluorescence signals. Therefore, this technique can be confidently used to directly monitor parasitic load and infection overtime and could be an excellent tool for in vitro and in vivo screening of anti-leishmanial drugs and vaccine efficiency. This is the first report of the use of the near-infrared fluorescence imaging technique for monitoring in vivo cutaneous leishmaniasis.

  3. Formação de professores na prática política do MST: a construção da consciência orgulhosa Teacher education in the political practice of the MST: the construction of the proud consciousness

    Directory of Open Access Journals (Sweden)

    Sonia Aparecida Branco Beltrame

    2002-07-01

    the agents involved, highlighting the relationships built in family life, in the exercise of the profession, and in the political participation in the Movement. The analysis of the actions carried out by the teachers in various spheres reveals how they become agents of their own experience. Joining the education proposals of the MST allows in these teachers the manifestation of an identity that strengthens the everyday experience of being a teacher, promoting the development of a proud consciousness of the teaching practice. The experience is fed back to the school and to other educational opportunities promoted by the MST, to the contact with the group of teachers, to the studies they carry out, and to the exchanges with students and their families. That allows teachers to set priorities, adding significant elements to the contents seen at school. The prospect is then revealed for the transmission at school of a dimension of knowledge enhanced by the political action engendered in collective practices. This involvement results in differentiated experiences with benefit to the actions developed at school and in the Movement. Thus, these teachers re-establish the role of the school in the rural areas, creating new commitments that transform their teaching activity, broadening their learning and the relationships of solidarity present in the countryside culture.

  4. Implementation of Evidence-Based Models in Social Work Practice: Practitioners' Perspectives on an MST Trial in Sweden

    Science.gov (United States)

    Gustle, Lars-Henry; Hansson, Kjell; Sundell, Knut; Andree-Lofholm, Cecilia

    2008-01-01

    The implementation of new treatment methods in social work practice is warranted. Moreover, little is known about professionals' attitudes toward the introduction of evidence-based practices into their communities. Therefore, this article reports on the implementation of a Swedish research project that evaluated Multisystemic Therapy (MST). All…

  5. MST 1: Proceedings of a conference on the integration of mathematics, science and technology in precollege education

    Energy Technology Data Exchange (ETDEWEB)

    Swyler, K. [ed.

    1995-11-01

    Example MST activities examined here show: (1) an inquiry-driven learning stimulus, involving (2) the synthesis of concepts in math, science and technology, through (3) the application of the scientific method and engineering problem solving/test protocols, and provoking (4) a stimulus for further exploration. A semi-exploratory learning approach offered background aimed at enabling participants to take meaningful courses of investigation; this approach must be balanced by maintaining contact with framework content standards. On the whole, the philosophy underlying the MST learning approach--as envisioned in the draft NYS Framework, and embodied in the example activities--is strongly endorsed. This endorsement is broad-based: those represented include teachers of mathematics, science, and technology, and school district administrators--in roughly equal numbers. Discussion centers not on whether the MST approach should be pursued, but on what is involved in doing it. Teams of conference participants were given time to plan or extend MST initiatives in their own districts. Outlines of the initiatives proposed by ten of the teams are disseminated herein.

  6. A emergência do sujeito desejante no discurso do MST

    OpenAIRE

    Indursky, Freda

    2013-01-01

    No presente trabalho, retorno às designações ocupação/ invasão para examinar um registro muito peculiar da designação invasão, ocorrido em uma entrevista com Diolinda Alves de Souza, líder do MST, em 06/12/1995, para a Revista da Folha. Interessou- me, nesta entrevista, examinar o processo de subjetivação/identificação de Diolinda: em um determinado momento da referida entrevista, ao responder sobre sua primeira ocupação, refere-a como invasão. Assim procedendo, a entrevistada não mobiliza o ...

  7. Guillotine subdivisions approximate polygonal subdivisions: A simple new method for the geometric k-MST problem

    Energy Technology Data Exchange (ETDEWEB)

    Mitchell, J.S.B. [State Univ. of New York, Stony Brook, NY (United States)

    1996-12-31

    We show that any rectilinear polygonal subdivision in the plane can be converted into a {open_quotes}guillotine{close_quote} subdivision whose length is at most twice that of the original subdivision. {open_quote}Guillotine{close_quotes} subdivisions have a simple recursive structure that allows one to search for {open_quotes}optimal{close_quotes} such subdivisions in polynomial time, using dynamic programming. In particular, a consequence of our main theorem is a very simple proof that the k-MST problem in the plane has a constant factor polynomial-time approximation algorithm, and the constant factor that we obtain is a substantial improvement over all previous bounds: We obtain a factor of 2 for the L{sub 1} metric, and a factor of 2{radical}2 for the L{sub 2} (Euclidean) metric.

  8. Symposium Review: Metal and Polymer Matrix Composites at MS&T 2013

    Science.gov (United States)

    Gupta, Nikhil; Paramsothy, Muralidharan

    2014-06-01

    This article reflects on the presentations made during the Metal and Polymer Matrix Composites symposium at Materials Science and Technology 2013 (MS&T'13) held in Montreal (Quebec, Canada) from October 27 to 31. The symposium had three sessions on metal matrix composites and one session on polymer matrix composites containing a total of 23 presentations. While the abstracts and full-text papers are available through databases, the discussion that took place during the symposium is often not captured in writing and gets immediately lost. We have tried to recap some of the discussion in this article and hope that it will supplement the information present in the proceedings. The strong themes in the symposium were porous composites, aluminum matrix composites, and nanocomposites. The development of processing methods was also of interest to the speakers and attendees.

  9. Techniques And Results For The Calibration Of The MST Prototype For The Cherenkov Telescope Array

    CERN Document Server

    ,

    2016-01-01

    The next generation instrument for ground-based gamma-ray astronomy will be the Cherenkov Telescope Array (CTA), consisting of approximately 100 telescopes in three sizes, built on two sites with one each in the Northern and Southern Hemi- spheres. Up to 40 of these will be Medium Size Telescopes (MSTs) which will dominate sensitivity in the core energy range. Since 2012, a full size mechanical prototype for the modified 12 m Davies-Cotton design MST has been in operation in Berlin. This doc- ument describes the techniques which have been implemented to calibrate and optimise the mechanical and optical performance of the prototype, and gives the results of over three years of observations and measurements. Pointing calibration techniques will be discussed, along with the development of a bending model, and calibration of the CCD cameras used for pointing measurements. Additionally alignment of mirror segments using the Bokeh method is shown.

  10. DLG5 connects cell polarity and Hippo signaling protein networks by linking PAR-1 with MST1/2.

    Science.gov (United States)

    Kwan, Julian; Sczaniecka, Anna; Arash, Emad Heidary; Nguyen, Liem; Chen, Chia-Chun; Ratkovic, Srdjana; Klezovitch, Olga; Attisano, Liliana; McNeill, Helen; Emili, Andrew; Vasioukhin, Valeri

    2016-12-15

    Disruption of apical-basal polarity is implicated in developmental disorders and cancer; however, the mechanisms connecting cell polarity proteins with intracellular signaling pathways are largely unknown. We determined previously that membrane-associated guanylate kinase (MAGUK) protein discs large homolog 5 (DLG5) functions in cell polarity and regulates cellular proliferation and differentiation via undefined mechanisms. We report here that DLG5 functions as an evolutionarily conserved scaffold and negative regulator of Hippo signaling, which controls organ size through the modulation of cell proliferation and differentiation. Affinity purification/mass spectrometry revealed a critical role of DLG5 in the formation of protein assemblies containing core Hippo kinases mammalian ste20 homologs 1/2 (MST1/2) and Par-1 polarity proteins microtubule affinity-regulating kinases 1/2/3 (MARK1/2/3). Consistent with this finding, Hippo signaling is markedly hyperactive in mammalian Dlg5(-/-) tissues and cells in vivo and ex vivo and in Drosophila upon dlg5 knockdown. Conditional deletion of Mst1/2 fully rescued the phenotypes of brain-specific Dlg5 knockout mice. Dlg5 also interacts genetically with Hippo effectors Yap1/Taz Mechanistically, we show that DLG5 inhibits the association between MST1/2 and large tumor suppressor homologs 1/2 (LATS1/2), uses its scaffolding function to link MST1/2 with MARK3, and inhibits MST1/2 kinase activity. These data reveal a direct connection between cell polarity proteins and Hippo, which is essential for proper development of multicellular organisms.

  11. Mst2 and Lats kinases regulate apoptotic function of Yes kinase-associated protein (YAP).

    Science.gov (United States)

    Oka, Tsutomu; Mazack, Virginia; Sudol, Marius

    2008-10-10

    The Hippo pathway in Drosophila controls the size and shape of organs. In the fly, activation of this pathway conveys growth-inhibitory signals and promotes apoptosis in epithelial cells. We "reconstituted" the Hippo pathway in a human epithelial cell line and showed that, in contrast to flies, the activation of this pathway results in anti-apoptotic signals. We have shown that in human embryonic kidney (HEK) 293 cells, the complex formation between transcriptional co-activators YAPs (Yes kinase-associated proteins) and Lats kinases requires the intact WW domains of YAPs, as well as intact Pro-Pro-AA-Tyr (where AA is any amino acid) motifs in Lats kinases. These kinases cooperate with the upstream Mst2 kinase to phosphorylate YAPs at Ser-127. Overexpression of YAP2 in HEK293 cells promoted apoptosis, whereas the Mst2/Lats1-induced phosphorylation of YAP partially rescued the cells from apoptotic death. Apoptotic signaling of YAP2 was mediated via stabilization of p73, which formed a complex with YAP2. All components of the Hippo pathway that we studied were localized in the cytoplasm, with the exception of YAP, which also localized in the nucleus. The localization of YAP2 in the nucleus was negatively controlled by the Lats1 kinase. Our apoptotic "readout" of the Hippo pathway in embryonic kidney cells represents a useful experimental system for the identification of the putative upstream receptor, membrane protein, or extracellular factor that initiates an entire signaling cascade and ultimately controls the size of organs.

  12. Proper actin ring formation and septum constriction requires coordinated regulation of SIN and MOR pathways through the germinal centre kinase MST-1.

    Science.gov (United States)

    Heilig, Yvonne; Dettmann, Anne; Mouriño-Pérez, Rosa R; Schmitt, Kerstin; Valerius, Oliver; Seiler, Stephan

    2014-04-01

    Nuclear DBF2p-related (NDR) kinases constitute a functionally conserved protein family of eukaryotic regulators that control cell division and polarity. In fungi, they function as effector kinases of the morphogenesis (MOR) and septation initiation (SIN) networks and are activated by pathway-specific germinal centre (GC) kinases. We characterized a third GC kinase, MST-1, that connects both kinase cascades. Genetic and biochemical interactions with SIN components and life cell imaging identify MST-1 as SIN-associated kinase that functions in parallel with the GC kinase SID-1 to activate the SIN-effector kinase DBF-2. SID-1 and MST-1 are both regulated by the upstream SIN kinase CDC-7, yet in an opposite manner. Aberrant cortical actomyosin rings are formed in Δmst-1, which resulted in mis-positioned septa and irregular spirals, indicating that MST-1-dependent regulation of the SIN is required for proper formation and constriction of the septal actomyosin ring. However, MST-1 also interacts with several components of the MOR network and modulates MOR activity at multiple levels. MST-1 functions as promiscuous enzyme and also activates the MOR effector kinase COT-1 through hydrophobic motif phosphorylation. In addition, MST-1 physically interacts with the MOR kinase POD-6, and dimerization of both proteins inactivates the GC kinase hetero-complex. These data specify an antagonistic relationship between the SIN and MOR during septum formation in the filamentous ascomycete model Neurospora crassa that is, at least in part, coordinated through the GC kinase MST-1. The similarity of the SIN and MOR pathways to the animal Hippo and Ndr pathways, respectively, suggests that intensive cross-communication between distinct NDR kinase modules may also be relevant for the homologous NDR kinases of higher eukaryotes.

  13. Proper actin ring formation and septum constriction requires coordinated regulation of SIN and MOR pathways through the germinal centre kinase MST-1.

    Directory of Open Access Journals (Sweden)

    Yvonne Heilig

    2014-04-01

    Full Text Available Nuclear DBF2p-related (NDR kinases constitute a functionally conserved protein family of eukaryotic regulators that control cell division and polarity. In fungi, they function as effector kinases of the morphogenesis (MOR and septation initiation (SIN networks and are activated by pathway-specific germinal centre (GC kinases. We characterized a third GC kinase, MST-1, that connects both kinase cascades. Genetic and biochemical interactions with SIN components and life cell imaging identify MST-1 as SIN-associated kinase that functions in parallel with the GC kinase SID-1 to activate the SIN-effector kinase DBF-2. SID-1 and MST-1 are both regulated by the upstream SIN kinase CDC-7, yet in an opposite manner. Aberrant cortical actomyosin rings are formed in Δmst-1, which resulted in mis-positioned septa and irregular spirals, indicating that MST-1-dependent regulation of the SIN is required for proper formation and constriction of the septal actomyosin ring. However, MST-1 also interacts with several components of the MOR network and modulates MOR activity at multiple levels. MST-1 functions as promiscuous enzyme and also activates the MOR effector kinase COT-1 through hydrophobic motif phosphorylation. In addition, MST-1 physically interacts with the MOR kinase POD-6, and dimerization of both proteins inactivates the GC kinase hetero-complex. These data specify an antagonistic relationship between the SIN and MOR during septum formation in the filamentous ascomycete model Neurospora crassa that is, at least in part, coordinated through the GC kinase MST-1. The similarity of the SIN and MOR pathways to the animal Hippo and Ndr pathways, respectively, suggests that intensive cross-communication between distinct NDR kinase modules may also be relevant for the homologous NDR kinases of higher eukaryotes.

  14. Measurements of Electric Field Fluctuations Using a Capacitive Probe on the MST Reversed Field Pinch

    Science.gov (United States)

    Tan, Mingsheng; Almagri, A. F.; Sarff, J. S.; McCollam, K. J.; Triana, J. C.; Li, H.; Ding, W. X.; Liu, W.

    2015-11-01

    Experimental measurements and extended MHD computation reveal that both flow and current density fluctuations are important for the magnetic relaxation of RFP plasmas via tearing fluctuations. Motivated by these results, we have developed a multi-electrode capacitive probe for radial profile measurements of the electrostatic potential deep in the plasma. The capacitive probe measures the ac plasma potential via electrodes insulated from the plasma using an annular boron nitride dielectric (also the particle shield), provided the secondary emission is sufficiently large (Te>20 eV). The probe has ten sets of four capacitors with 1.5 cm radial separation. At each radius, four capacitors are arranged on a 1.3 cm square grid. This probe has been inserted up to 15 cm from the wall in 200 kA deuterium plasmas. The fluctuation amplitudes increase during the sawtooth crash and the power spectrum broadens (similar to the behavior of magnetic field fluctuations). The frequency bandwidth allows measurements of the radial coherence and phase of the fluctuations associated with rotating tearing modes up to the Alfvénic range. A next-step goal is measurement of the total dynamo emf, ~ /B0 , to complement ongoing measurements of the Hall dynamo emf, / ne , using a deep-insertion magnetic probe. M. Tan is supported by ITER-China Program. Work is supported by US DOE.

  15. Heterogeneous transgene expression in the retinas of the TH-RFP, TH-Cre, TH-BAC-Cre and DAT-Cre mouse lines.

    Science.gov (United States)

    Vuong, H E; Pérez de Sevilla Müller, L; Hardi, C N; McMahon, D G; Brecha, N C

    2015-10-29

    Transgenic mouse lines are essential tools for understanding the connectivity, physiology and function of neuronal circuits, including those in the retina. This report compares transgene expression in the retina of a tyrosine hydroxylase (TH)-red fluorescent protein (RFP) mouse line with three catecholamine-related Cre recombinase mouse lines [TH-bacterial artificial chromosome (BAC)-, TH-, and dopamine transporter (DAT)-Cre] that were crossed with a ROSA26-tdTomato reporter line. Retinas were evaluated and immunostained with commonly used antibodies including those directed to TH, GABA and glycine to characterize the RFP or tdTomato fluorescent-labeled amacrine cells, and an antibody directed to RNA-binding protein with multiple splicing to identify ganglion cells. In TH-RFP retinas, types 1 and 2 dopamine (DA) amacrine cells were identified by their characteristic cellular morphology and type 1 DA cells by their expression of TH immunoreactivity. In the TH-BAC-, TH-, and DAT-tdTomato retinas, less than 1%, ∼ 6%, and 0%, respectively, of the fluorescent cells were the expected type 1 DA amacrine cells. Instead, in the TH-BAC-tdTomato retinas, fluorescently labeled AII amacrine cells were predominant, with some medium diameter ganglion cells. In TH-tdTomato retinas, fluorescence was in multiple neurochemical amacrine cell types, including four types of polyaxonal amacrine cells. In DAT-tdTomato retinas, fluorescence was in GABA immunoreactive amacrine cells, including two types of bistratified and two types of monostratified amacrine cells. Although each of the Cre lines was generated with the intent to specifically label DA cells, our findings show a cellular diversity in Cre expression in the adult retina and indicate the importance of careful characterization of transgene labeling patterns. These mouse lines with their distinctive cellular labeling patterns will be useful tools for future studies of retinal function and visual processing.

  16. Review of magnetostrictive transducers (MsT) utilizing reversed Wiedemann effect

    Science.gov (United States)

    Vinogradov, Sergey; Cobb, Adam; Light, Glenn

    2017-02-01

    Magnetostrictive transduction has been widely utilized in NDE applications, specifically for generation and reception of guided waves for long-range inspection of components such as pipes, vessels, and small tubes. Transverse-motion guided wave modes (e.g., torsional vibrations in pipes) are the most typical choice for long-range inspection applications because the wave motion is in the plane of the structure. Magnetostrictive-based sensors have been available for several years for these wave modes based on the Wiedemann effect. For these sensors, a permanent magnetic bias is applied that is perpendicular to the direction of the propagated guided wave. This bias field strains the material that the guided wave is generated in preferentially in the desired particle motion direction. A time-varying magnetic field oriented parallel to the direction of guided wave propagation is also induced in the material. This time-varying field is induced using an electric coil located near the material surface. The interaction of these two fields produces the guided waves; an inverse effect is used for the receive process. An alternative configuration of a sensor for generating and receiving these traverse-motion guided waves is to swap the biasing and time-varying magnetic fields directions. Since transverse-motion guided wave sensors are typically much longer in the particle motion direction than in the bias field direction, the net effect of this alternative design is that the magnetic biasing length is shorter and different coil designs can be used. Because of this, the alternative design, known as a magnetostrictive transducer (MsT), exhibits a number of unique features compared to the Wiedemann sensor described above, such as: 1) the ability to use smaller rare earth permanent magnets and achieve uniform and self-sustained bias field strengths, 2) the choice of more efficient electric coil arrangements to induce a stronger time-varying magnetic field for a given coil

  17. Identification and mitigation of stray laser light in the Thomson scattering system on the Madison Symmetric Torus (MST)

    Energy Technology Data Exchange (ETDEWEB)

    Jacobson, C. M., E-mail: cjacobson@wisc.edu; Borchardt, M. T.; Den Hartog, D. J.; Falkowski, A. F.; Morton, L. A.; Thomas, M. A. [Department of Physics, University of Wisconsin–Madison, 1150 University Avenue, Madison, Wisconsin 53706 (United States)

    2016-11-15

    The Thomson scattering diagnostic on the Madison Symmetric Torus (MST) records excessive levels of stray Nd:YAG laser light. Stray light saturates the 1064 nm spectral channel in all polychromators, which prevents absolute electron density measurements via Rayleigh scattering calibration. Furthermore, stray light contaminates adjacent spectral channels for r/a ≥ 0.75, which renders the diagnostic unable to make electron temperature measurements at these radii. In situ measurements of stray light levels during a vacuum vessel vent are used to identify stray light sources and strategies for reduction of stray light levels. Numerical modeling using Zemax OpticStudio supports these measurements. The model of the vacuum vessel and diagnostic includes synthetic collection optics to enable direct comparison of measured and simulated stray light levels. Modeling produces qualitatively similar stray light distributions to MST measurements, and quantifies the mitigation effects of stray light mitigation strategies prior to implementation.

  18. Identification and mitigation of stray laser light in the Thomson scattering system on the Madison Symmetric Torus (MST)

    Science.gov (United States)

    Jacobson, C. M.; Borchardt, M. T.; Den Hartog, D. J.; Falkowski, A. F.; Morton, L. A.; Thomas, M. A.

    2016-11-01

    The Thomson scattering diagnostic on the Madison Symmetric Torus (MST) records excessive levels of stray Nd:YAG laser light. Stray light saturates the 1064 nm spectral channel in all polychromators, which prevents absolute electron density measurements via Rayleigh scattering calibration. Furthermore, stray light contaminates adjacent spectral channels for r/a ≥ 0.75, which renders the diagnostic unable to make electron temperature measurements at these radii. In situ measurements of stray light levels during a vacuum vessel vent are used to identify stray light sources and strategies for reduction of stray light levels. Numerical modeling using Zemax OpticStudio supports these measurements. The model of the vacuum vessel and diagnostic includes synthetic collection optics to enable direct comparison of measured and simulated stray light levels. Modeling produces qualitatively similar stray light distributions to MST measurements, and quantifies the mitigation effects of stray light mitigation strategies prior to implementation.

  19. PILOT-SCALE TESTING OF THE SUSPENSION OF MST, CST, AND SIMULATED SLUDGE SLURRIES IN A SLUDGE TANK

    Energy Technology Data Exchange (ETDEWEB)

    Poirier, M.; Qureshi, Z.; Restivo, M.; Steeper, T.; Williams, M.; Herman, D.

    2011-08-02

    The Small Column Ion Exchange (SCIX) process is being developed to remove cesium, strontium, and actinides from Savannah River Site (SRS) Liquid Waste using an existing waste tank (i.e., Tank 41H) to house the process. Following strontium, actinide, and cesium removal, the concentrated solids will be transported to a sludge tank (i.e., monosodium titanate (MST)/sludge solids to Tank 42H or Tank 51H and crystalline silicotitanate (CST) to Tank 40H) for eventual transfer to the Defense Waste Processing Facility (DWPF). Savannah River National Laboratory (SRNL) is conducting pilot-scale mixing tests to determine the pump requirements for mixing MST, CST, and simulated sludge. The purpose of this pilot scale testing is to determine the pump requirements for mixing MST and CST with sludge in a sludge tank and to determine whether segregation of particles occurs during settling. Tank 40H and Tank 51H have four Quad Volute pumps; Tank 42H has four standard pumps. The pilot-scale tank is a 1/10.85 linear scaled model of Tank 40H. The tank diameter, tank liquid level, pump nozzle diameter, pump elevation, and cooling coil diameter are all 1/10.85 of their dimensions in Tank 40H. The pump locations correspond to the current locations in Tank 40H (Risers B2, H, B6, and G). The pumps are pilot-scale Quad Volute pumps. Additional settling tests were conducted in a 30 foot tall, 4 inch inner diameter clear column to investigate segregation of MST, CST, and simulated sludge particles during settling.

  20. Pequenos lutadores: um estudo sobre a construção da infância no interior do MST

    Directory of Open Access Journals (Sweden)

    Deise Arenhart

    2005-01-01

    Full Text Available O estudo ora relatado está situado no campo das reflexões sobre infância, educação e movimentos sociais. Tendo como suporte a Pedagogia do MST, da qual destaco a luta, a mística e o trabalho, analiso os significados que as crianças atribuem a esses elementos pedagógicos e a forma como os produzem, relativas a sua condição infantil.

  1. Sample results from the interim salt disposition program macrobatch 8 tank 21H qualification MST solids sample

    Energy Technology Data Exchange (ETDEWEB)

    Peters, T. B. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Washington, A. L. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL)

    2015-02-01

    Savannah River National Laboratory (SRNL) analyzed samples from Tank 21H in support of qualification of Macrobatch (Salt Batch) 8 for Interim Salt Disposition Program (ISDP) processing. As part of this qualification work, SRNL performed an Actinide Removal Process (ARP) and several Extraction, Scrub, Strip (ESS) tests. This document reports characterization of the monosodium titanate (MST) solids from the ARP test. The results of these analyses are reported and are within historical precedent.

  2. Discovery and Mechanistic Characterization of Selective Inhibitors of H2S-producing Enzyme: 3-Mercaptopyruvate Sulfurtransferase (3MST) Targeting Active-site Cysteine Persulfide

    Science.gov (United States)

    Hanaoka, Kenjiro; Sasakura, Kiyoshi; Suwanai, Yusuke; Toma-Fukai, Sachiko; Shimamoto, Kazuhito; Takano, Yoko; Shibuya, Norihiro; Terai, Takuya; Komatsu, Toru; Ueno, Tasuku; Ogasawara, Yuki; Tsuchiya, Yukihiro; Watanabe, Yasuo; Kimura, Hideo; Wang, Chao; Uchiyama, Masanobu; Kojima, Hirotatsu; Okabe, Takayoshi; Urano, Yasuteru; Shimizu, Toshiyuki; Nagano, Tetsuo

    2017-01-01

    Very recent studies indicate that sulfur atoms with oxidation state 0 or −1, called sulfane sulfurs, are the actual mediators of some physiological processes previously considered to be regulated by hydrogen sulfide (H2S). 3-Mercaptopyruvate sulfurtransferase (3MST), one of three H2S-producing enzymes, was also recently shown to produce sulfane sulfur (H2Sn). Here, we report the discovery of several potent 3MST inhibitors by means of high-throughput screening (HTS) of a large chemical library (174,118 compounds) with our H2S-selective fluorescent probe, HSip-1. Most of the identified inhibitors had similar aromatic ring-carbonyl-S-pyrimidone structures. Among them, compound 3 showed very high selectivity for 3MST over other H2S/sulfane sulfur-producing enzymes and rhodanese. The X-ray crystal structures of 3MST complexes with two of the inhibitors revealed that their target is a persulfurated cysteine residue located in the active site of 3MST. Precise theoretical calculations indicated the presence of a strong long-range electrostatic interaction between the persulfur anion of the persulfurated cysteine residue and the positively charged carbonyl carbon of the pyrimidone moiety of the inhibitor. Our results also provide the experimental support for the idea that the 3MST-catalyzed reaction with 3-mercaptopyruvate proceeds via a ping-pong mechanism. PMID:28079151

  3. An Alternative to Optimize the Indonesian’s Airport Network Design: An Application of Minimum Spanning Tree (MST Technique

    Directory of Open Access Journals (Sweden)

    Luluk Lusiantoro

    2012-09-01

    Full Text Available Using minimum spanning tree technique (MST, this exploratory research was done to optimize the interrelation and hierarchical network design of Indonesian’s airports. This research also identifies the position of the Indonesian’s airports regionally based on the ASEAN Open Sky Policy 2015. The secondary data containing distance between airports (both in Indonesia and in ASEAN, flight frequency, and correlation of Gross Domestic Regional Product (GDRP for each region in Indonesia are used as inputs to form MST networks. The result analysis is done by comparing the MST networks with the existing network in Indonesia. This research found that the existing airport network in Indonesia does not depict the optimal network connecting all airports with the shortest distance and maximizing the correlation of regional economic potential in the country. This research then suggests the optimal networks and identifies the airports and regions as hubs and spokes formed by the networks. Lastly, this research indicates that the Indonesian airports have no strategic position in the ASEAN Open Sky network, but they have an opportunity to get strategic positions if 33 airports in 33 regions in Indonesia are included in the network.

  4. The role of local and remote amino acid substitutions for optimizing fluorescence in bacteriophytochromes: A case study on iRFP.

    Science.gov (United States)

    Buhrke, David; Velazquez Escobar, Francisco; Sauthof, Luisa; Wilkening, Svea; Herder, Nico; Tavraz, Neslihan N; Willoweit, Mario; Keidel, Anke; Utesch, Tillmann; Mroginski, Maria-Andrea; Schmitt, Franz-Josef; Hildebrandt, Peter; Friedrich, Thomas

    2016-06-22

    Bacteriophytochromes are promising tools for tissue microscopy and imaging due to their fluorescence in the near-infrared region. These applications require optimization of the originally low fluorescence quantum yields via genetic engineering. Factors that favour fluorescence over other non-radiative excited state decay channels are yet poorly understood. In this work we employed resonance Raman and fluorescence spectroscopy to analyse the consequences of multiple amino acid substitutions on fluorescence of the iRFP713 benchmark protein. Two groups of mutations distinguishing iRFP from its precursor, the PAS-GAF domain of the bacteriophytochrome P2 from Rhodopseudomonas palustris, have qualitatively different effects on the biliverdin cofactor, which exists in a fluorescent (state II) and a non-fluorescent conformer (state I). Substitution of three critical amino acids in the chromophore binding pocket increases the intrinsic fluorescence quantum yield of state II from 1.7 to 5.0% due to slight structural changes of the tetrapyrrole chromophore. Whereas these changes are accompanied by an enrichment of state II from ~40 to ~50%, a major shift to ~88% is achieved by remote amino acid substitutions. Additionally, an increase of the intrinsic fluorescence quantum yield of this conformer by ~34% is achieved. The present results have important implications for future design strategies of biofluorophores.

  5. The role of local and remote amino acid substitutions for optimizing fluorescence in bacteriophytochromes: A case study on iRFP

    Science.gov (United States)

    Buhrke, David; Velazquez Escobar, Francisco; Sauthof, Luisa; Wilkening, Svea; Herder, Nico; Tavraz, Neslihan N.; Willoweit, Mario; Keidel, Anke; Utesch, Tillmann; Mroginski, Maria-Andrea; Schmitt, Franz-Josef; Hildebrandt, Peter; Friedrich, Thomas

    2016-01-01

    Bacteriophytochromes are promising tools for tissue microscopy and imaging due to their fluorescence in the near-infrared region. These applications require optimization of the originally low fluorescence quantum yields via genetic engineering. Factors that favour fluorescence over other non-radiative excited state decay channels are yet poorly understood. In this work we employed resonance Raman and fluorescence spectroscopy to analyse the consequences of multiple amino acid substitutions on fluorescence of the iRFP713 benchmark protein. Two groups of mutations distinguishing iRFP from its precursor, the PAS-GAF domain of the bacteriophytochrome P2 from Rhodopseudomonas palustris, have qualitatively different effects on the biliverdin cofactor, which exists in a fluorescent (state II) and a non-fluorescent conformer (state I). Substitution of three critical amino acids in the chromophore binding pocket increases the intrinsic fluorescence quantum yield of state II from 1.7 to 5.0% due to slight structural changes of the tetrapyrrole chromophore. Whereas these changes are accompanied by an enrichment of state II from ~40 to ~50%, a major shift to ~88% is achieved by remote amino acid substitutions. Additionally, an increase of the intrinsic fluorescence quantum yield of this conformer by ~34% is achieved. The present results have important implications for future design strategies of biofluorophores. PMID:27329837

  6. Control of the wax moth Galleria mellonella L. (Lepidoptera: Pyralidae by the male sterile technique (MST

    Directory of Open Access Journals (Sweden)

    Jafari Reza

    2010-01-01

    Full Text Available In this study we examined the control of wax moth using the male sterile technique (MST with gamma-rays. To determine the safe and effective dosage of gamma-rays capable of sterilizing male pupae of the wax moth, male pupae were exposed to increasing single doses of gamma-rays (250, 300, 350 and 400 Gy. The release ratio of sterile to normal males was also studied in a similar experiment. Treatments included sterile males, normal males and virgin females at the following ratios: 1:1:1, 2:1:1, 3:1:1, 4:1:1 and 5:1:1. Possible parthenogenetic reproduction of this pest was also examined. The results showed that 350 Gy was the most effective dose capable of sterilizing the male pupae of the wax moth. The best release ratio was established at four sterile males, one normal male for each normal female (4:1:1. Also females were incapable of producing offspring without males.

  7. SUT Sucrose and MST Monosaccharide Transporter Inventory of the Selaginella Genome.

    Science.gov (United States)

    Lalonde, Sylvie; Frommer, Wolf B

    2012-01-01

    Most metazoa use hexose transporters to acquire hexoses from their diet and as a transport form for distributing carbon and energy within their bodies; insects use trehalose, and plants use sucrose as their major form for translocation. Plant genomes contain at least three families of mono- and disaccharide transporters: monosaccharide/polyol transporters that are evolutionary closely related to the yeast and human glucose transporters, sucrose transporters of the SUT family, which similar to the hexose transporters belong to the major facilitator superfamily, but share only minimal amino acid sequence homology with the hexose transporters, and the family of SWEET sugar transporters conserved between animals and plants. Recently, the genome sequence of the spikemoss Selaginella has been determined. In order to study the evolution of sugar transport in plants, we carefully annotated of the complement of sugar transporters in Selaginella. We review the current knowledge regarding sugar transport in spikemoss and provide phylogenetic analyses of the complement of MST and SUT homologs in Selaginella (and Physcomitrella).

  8. SUT sucrose and MST monosaccharide transporter inventory of the Selaginella genome

    Directory of Open Access Journals (Sweden)

    Sylvie eLalonde

    2012-02-01

    Full Text Available While most metazoa mainly use hexose transporters to acquire hexoses from their diet and as a transport form for distributing carbon and energy within their bodies, insects use trehalose and plants use sucrose as their major form for translocation. Plant genomes contain at least three families of mono- and disaccharide transporters: monosaccharide/polyol transporters that are evolutionary closely related to the yeast and human glucose transporters, sucrose transporters of the SUT family, which similar to the hexose transporters belong to the major facilitator superfamily (MFS, but share only minimal amino acid sequence homology to the hexose transporters, and the family of SWEET sugar transporters conserved between animals and plants. Recently, the genome sequence of the spikemoss Selaginella has been determined. In order to study the evolution of sugar transport in plants, we carried out a careful annotation of the complement of sugar transporters in Selaginella. We review what is known about sugar transport in spikemoss and provide phylogenetic analyses of the complement of MST and SUT homologs in Selaginella.

  9. Upgrades to improve the usability, reliability, and spectral range of the MST Thomson scattering diagnostic

    Science.gov (United States)

    Kubala, S. Z.; Borchardt, M. T.; Den Hartog, D. J.; Holly, D. J.; Jacobson, C. M.; Morton, L. A.; Young, W. C.

    2016-11-01

    The Thomson scattering diagnostic on MST records both equilibrium and fluctuating electron temperature with a range capability of 10 eV-5 keV. Standard operation with two modified commercial Nd:YAG lasers allows measurements at rates of 1 kHz-25 kHz. Several subsystems of the diagnostic are being improved. The power supplies for the avalanche photodiode detectors (APDs) that record the scattered light are being replaced to improve usability, reliability, and maintainability. Each of the 144 APDs will have an individual rack mounted switching supply, with bias voltage adjustable to match the APD. Long-wavelength filters (1140 nm center, 80 nm bandwidth) have been added to the polychromators to improve capability to resolve non-Maxwellian distributions and to enable directed electron flow measurements. A supercontinuum (SC) pulsed white light source has replaced the tungsten halogen lamp previously used for spectral calibration of the polychromators. The SC source combines substantial brightness produced in nanosecond pulses with a spectrum that covers the entire range of the polychromators.

  10. Impact of diagnostic neutral beam optimization on active spectroscopy in MST

    Science.gov (United States)

    Feng, Xiande; Nornberg, Mark. D.; den Hartog, Daniel. J.; Oliva, Steven. P.; Craig, Darren; Univ of Wisconsin, Madison Team; Wheaton College, IL Collaboration

    2016-10-01

    The hydrogen diagnostic neutral beam on MST provides local measurements of impurity ion emission through charge exchange recombination spectroscopy (CHERS) and of core-localized magnetic field through the motional Stark effect (MSE). The beam has been optimized to operate at 50kV, 4A steady beam current with 20ms beam pulse and 75% primary energy ion fraction. It's achieved by tuning the beam voltage, arc current, fuel line pressure, arc and high voltage module timing, and the magnetic isolation field. Electron density measurements in the ion source revealed that ion extraction is maximized under low density conditions which are thought to affect the shape of the ion sheath at the extraction grid. The sheath may be transitioning from a planar or convex shape at high density to one which is concave which helps focus the ion trajectories and produce higher beam current. With the improvements in beam operation, the CHERS signal is expected to increase by 20%-30%, and the Stark broadening is expected to increase by 10%. These signal increases will help resolve convolved fine-structure components in both analyses. Beam voltage ripple is also measured to better quantify the accuracy of spectral MSE and CHERS measurement. This work is supported by the U.S. DOE.

  11. Design of an RF System for Electron Bernstein Wave Studies in MST

    Science.gov (United States)

    Kauffold, J. X.; Seltzman, A. H.; Anderson, J. K.; Nonn, P. D.; Forest, C. B.

    2010-11-01

    Motivated by the possibility of current profile control a 5.5GHz RF system for EBW is being developed. The central component is a standard radar Klystron with 1.2MW peak power and 4μs typical pulse length. Meaningful experiments require RF pulse lengths similar to the characteristic electron confinement times in MST necessitating the creation of a power supply providing 80kV at 40A for 10ms. A low inductance IGBT network switches power at 20kHz from an electrolytic capacitor bank into the primary of a three-phase resonant transformer system that is then rectified and filtered. The system uses three magnetically separate transformers with microcrystalline iron cores to provide suitable volt-seconds and low hysteresis losses. Each phase has a secondary with a large leakage inductance and a parallel capacitor providing a boost ratio greater than 60:1 with a physical turns ratio of 13.5:1. A microprocessor feedback control system varies the drive frequency around resonance to regulate the boost ratio and provide a stable output as the storage bank discharges. The completed system will deliver RF to the plasma boundary where coupling to the Bernstein mode and subsequent heating and current drive can occur.

  12. Evidence of Meteoroid Fragmentation in Specular Trail Echoes Observed Using Gadanki MST Radar

    Science.gov (United States)

    Patil, A.; Malhotra, A.; Patra, A. K.; Prasad, T. R.; Mathews, J. D.

    2015-02-01

    Meteoroids are responsible for deposition of thousands of kilograms of annual mass flux in the Earth's upper atmosphere but the disintegration mechanisms of these bodies, and hence their composition, still remains a subject of debate in the meteor radar community. The role and significance of fragmentation as a meteoroid disintegration mechanism has been of particular interest in the past few years but in contrast to the head echoes, relatively little work has been done to study the effect and extent of fragmentation on trail echoes observed by the high power large aperture radars. Using the 53 MHz Gadanki MST radar, we present examples of radar meteor trails whose evolution cannot be explained with just the aid of classical meteor ablation theory. These examples are analyzed and discussed on a case-by-case basis and it is reported that the evolution of these trails can be explained with the help of fragmentation. This study will form the basis for future modeling efforts of fragmenting meteor trails and has important implications on the form in which the meteoroid mass is deposited in the upper atmosphere.

  13. Fragmentation of specular overdense meteor trail echoes observed with Gadanki MST radar

    Science.gov (United States)

    Chenna Reddy, K.; Yellaiah, G.

    2016-02-01

    The pulse-integrated signal to noise ratio as a function of time known as radar meteor light curve (analogous to optical light curve), is an indicative of ablation processes during meteoroid flight in the atmosphere. In this study, we present and discuss few examples of light curves of long duration specular overdense meteor echoes detected with 53 MHz Gadanki (13.5°N, 79.2°E) MST radar. These echoes are of several seconds duration, where pulsation in amplitude is about ten cycles within few seconds. This means the fluctuations in amplitude are much slower than typical Fresnel oscillations of underdense as well as the head echo fluctuations. These light curves reveal several features unreported previously in the radar meteor returns that are consistent with meteoroid fragmentation. Some of them provide the strong observational evidence of a sub-millimeter-sized meteoroid, breaking apart into two distinct fragments. The pulsations in light curves are interpreted as being due to interference from two distinct scattering centers. Some other meteor events such as meteoroids undergoing quasi-continuous disintegration are also discussed.

  14. Paulo Freire e o MST: a Pedagogia do Oprimido no contexto da luta pela terra

    Directory of Open Access Journals (Sweden)

    Cristine Lima Pires

    2012-07-01

    Full Text Available Este artigo consiste de uma parte de um estudo sobre a formação humana no processo da luta pela terra, que constrói estratégias educativas não-formais e que re-significam os saberes populares, tendo como fundamentação teórica a Pedagogia do Oprimido de Paulo Freire. Com isso, buscou-se acompanhar o MST em diversas mobilizações, com o registro da dinâmica educativa não-formal, e as estratégias utilizadas no processo, com o intuito de analisar as possibilidades de uma educação emancipatória. Utilizou-se como metodologia de trabalho a história oral temática e imagética, e como instrumentos, as entrevistas com militantes e a filmagem da dinâmica organizativa que caracteriza a temática abordada. Conclui-se que ações educativas como essas são fundamentais e viabilizam a invenção de novas formas de sociabilidade, tendo o trabalho coletivo e democrático a solidariedade, a valorização pela vida, a defesa do trabalho e do estudo, enfim, formas sociais que constituem um exercício de educação para a consciência crítica e emancipatória.

  15. Looking for phase-space structures in star-forming regions: An MST-based methodology

    CERN Document Server

    Alfaro, Emilio J

    2015-01-01

    We present a method for analysing the phase space of star-forming regions. In particular we are searching for clumpy structures in the 3D subspace formed by two position coordinates and radial velocity. The aim of the method is the detection of kinematic segregated radial velocity groups, that is, radial velocity intervals whose associated stars are spatially concentrated. To this end we define a kinematic segregation index, $\\tilde{\\Lambda}$(RV), based on the Minimum Spanning Tree (MST) graph algorithm, which is estimated for a set of radial velocity intervals in the region. When $\\tilde{\\Lambda}$(RV) is significantly greater than 1 we consider that this bin represents a grouping in the phase space. We split a star-forming region into radial velocity bins and calculate the kinematic segregation index for each bin, and then we obtain the spectrum of kinematic groupings, which enables a quick visualization of the kinematic behaviour of the region under study. We carried out numerical models of different config...

  16. Tidal wind oscillations in the tropical lower atmosphere as observed by Indian MST Radar

    Directory of Open Access Journals (Sweden)

    M. N. Sasi

    Full Text Available Diurnal tidal components in horizontal winds measured by MST radar in the troposphere and lower stratosphere over a tropical station Gadanki (13.5° N, 79.2° E are presented for the autumn equinox, winter, vernal equinox and summer seasons. For this purpose radar data obtained over many diurnal cycles from September 1995 to August 1996 are used. The results obtained show that although the seasonal variation of the diurnal tidal amplitudes in zonal and meridional winds is not strong, vertical phase propagation characteristics show significant seasonal variation. An attempt is made to simulate the diurnal tidal amplitudes and phases in the lower atmosphere over Gadanki using classical tidal theory by incorporating diurnal heat sources, namely, solar radiation absorption by water vapour, planetary boundary layer (PBL heat flux, latent heat release in deep convective clouds and short wave solar radiation absorption by clouds. A comparison of the simulated amplitudes and phases with the observed ones shows that agreement between the two is quite good for the equinox seasons, especially the vertical structure of the phases of the meridional wind components.

    Key words. Meteorology and atmospheric dynamics (tropical meteorology; waves and tides

  17. Finland HF and Esrange MST radar observations of polar mesosphere summer echoes

    Directory of Open Access Journals (Sweden)

    T. Ogawa

    Full Text Available Peculiar near range echoes observed in summer with the SuperDARN HF radar in Finland are presented. The echoes were detected at four frequencies of 9, 11, 13 and 15 MHz at slant ranges of 105–250 km for about 100 min. Interferometer measurements indicate that the echoes are returned from 80–100 km altitudes with elevation angles of 20°–60°. Echo power (< 16 dB, Doppler velocity (between –30 and + 30 ms-1 and spectral width (< 60 ms-1 fluctuate with periods of several to 20 min, perhaps due to short–period atmospheric gravity waves. When the HF radar detected the echoes, a vertical incidence MST radar, located at Esrange in Sweden (650 km north of the HF radar site, observed polar mesosphere summer echoes (PMSE at altitudes of 80–90 km. This fact suggests that the near range HF echoes are PMSE at HF band, although both radars did not probe a common volume. With increasing radar frequency, HF echo ranges are closer to the radar site and echo power becomes weaker. Possible mechanisms to explain these features are discussed.

    Key words. Meteorology and atmospheric dynamics (middle atmosphere dynamics; thermospheric dynamics; waves and tides; instruments and techniques

  18. Association of MST4 gene promoter polymorphisms with Alzheimer's disease%MST-4基因启动子多态位点与AD的相关性研究

    Institute of Scientific and Technical Information of China (English)

    钟望涛; 赵斌; 吴志红; 陈煜森; 山县英玖; 三木哲郎

    2011-01-01

    Objective To explore the association of MST4(Serine/threonine-protein kinase) gene promoter and APOE polymorphisms with Alzheimer's disease. Methods Using the single nucleotide polymorphism(SNP) and TaqMan- PCR method the polymorphisms of MST4 gene in promoter(rs2748729 (3507A/G)) and APOE were analyzed in 377 AD(including 324 late-onset AD(LOAD) cases and 53 earlyonset AD(EOAD) cases) and 379 non-demented controls in Japanese population. Results (1)The frequencies of GG genotype in AD rs2748729 (-3507A/G) were significant lower than those in controls (0.28: 0. 35, P values were 0. 038), the same results can been seen among LOAD, EOAD, but P > 0.05;(2)Further comparative analysis between AD and controls among Non-APOE4 individuals showed that negative association of AD with rs2748729 GG genotype( P values were 0. 078). (3) Comparative analysis between AD and controls among female individuals demonstrated that negative association of AD with rs2748729 GG genotype. Conclusions The polymorphisms in promoter of MST4 were weakly significantly associated with lower AD risk, but it was negative result among female individuals,The ongoing investigation of the association in a larger sample and different ethnicities is warranted.%目的 探讨MST-4(丝/苏氨酸蛋白激酶-4)基因启动子区多态性、载脂蛋白E(ApoE)基因多态性和阿尔茨海默病(AD)的相关性.方法 通过使用TaqMan-PCR法检测单核苷酸多态性(SNP)方法,在377例日本人AD患者,包括324例迟发型AD(LOAD)与53例早发型AD(EOAD)和379例非痴呆对照组中观察MST4基因启动子rs2748729(-3507A/G)及APOE基因的多态性分布,并分析与AD的相关性.结果 (1)MST4基因启动子rs2748729上G/G纯合子频率在AD中明显低于对照组(0.28比0.35,P值为0.038);同样趋势见于LOAD,EOAD与对照组,但P值均>0.05;(2)进一步在非APOE4携带者中比较发现rs2748729上G/G纯合子频率在AD中亦低于对照组(0.27比0.35),但P值为0.078;(3)进一步性

  19. Assessment of analgesia in human chronic pain. Randomized double-blind crossover study of once daily repro-dose morphine versus MST continus.

    Science.gov (United States)

    Peat, S; Sweet, P; Miah, Y; Barklamb, M; Larsen, U

    1999-10-01

    This study evaluated Repro-Dose morphine (RDM; Reliadol from Nycomed Pharma), a new once daily controlled-release morphine formulation, against twice daily MST Continuous (MST) at steady state in patients with chronic opioid responsive pain. A randomized double-blind two-way crossover design was used to evaluate the efficacy and adverse effects of RDM once daily or MST twice daily, at the same total daily doses, in patients with chronic stable pain (dose range 20-120 mg per day). During the RDM limb of the study active drug was administered in the evening and placebo in the morning. Dextromoramide was provided as escape analgesia throughout the study. Following a 5-day screening period, during which stability of oral opioid dose was verified, patients underwent two 5-day treatment periods, (one MST, one RDM) in random sequence. Pain scores, escape analgesia requirements and side-effects were compared using data from days 3, 4 and 5 of each treatment period. Any events or medication changes occurring during the study period thought liable to influence analgesia were regarded as protocol violations. Overall assessment and period preference was assessed by direct questioning. RDM treatment was regarded as successful if the amount of escape medication required during the RDM period was equal to or less than that required during the MST period. Forty-seven patients were included in the study, of whom 40 completed both periods [the intention to treat (ITT) population], 31 in strict accordance with the protocol [the per protocol (PP) population]. Results were similar for both populations. There was no significant difference in pain scores or incidence of adverse events occurring during the MST and RDM periods. For the ITT population, requirements for escape medication during the RDM period were less than, equal to or greater than those recorded during the MST period for 14, 15, and 11 patients, respectively. Twenty-nine of 40 patients (72.5%) were therefore RDM treatment

  20. Spectroscopic measurement of the MHD dynamo in the MST reversed field pinch

    Energy Technology Data Exchange (ETDEWEB)

    Chapman, James Tharp [Univ. of Wisconsin, Madison, WI (United States)

    1998-09-01

    The author has directly observed the coupling of ion velocity fluctuations and magnetic field fluctuations to produce an MHD dynamo electric field in the interior of the MST reversed field pinch. Chord averaged ion velocity fluctuations were measured with a fast spectroscopic diagnostic which collects line radiation from intrinsic carbon impurities simultaneously along two lines of sight. The chords employed for the measurements resolved long wavelength velocity fluctuations of several km/s at 8-20 kHz as tiny, fast Doppler shifts in the emitted line profile. During discrete dynamo events the velocity fluctuations, like the magnetic fluctuations, increase dramatically. The toroidal and poloidal chords with impact parameters of 0.3 a and 0.6 a respectively, resolved fluctuation wavenumbers with resonance surfaces near or along the lines of sight indicating a radial velocity fluctuation width for each mode which spans only a fraction of the plasma radius. The phase between the measured toroidal velocity fluctuations and the magnetic fluctuations matches the predictions of resistive MHD while the poloidal velocity fluctuations exhibit a phase consistent with the superposition of MHD effects and the advection of a mean flow gradient past the poloidal line of sight. Radial velocity fluctuations resolved by a chord through the center of the plasma were small compared to the poloidal and toroidal fluctuations and exhibited low coherence with the magnetic fluctuations. The ensembled nonlinear product of the ion velocity fluctuations and fluctuations in the magnetic field indicates a substantial dynamo electric field which peaks during the periods of spontaneous flux generation.

  1. Compreendendo os movimentos sociais do campo e o MST: projeto educativo

    Directory of Open Access Journals (Sweden)

    Janaina Santana da Costa

    2016-12-01

    Full Text Available O presente artigo é síntese do texto final da dissertação de mestrado resultado da pesquisa realizada na Escola Paulo Freire do Assentamento Antônio Conselheiro situado no município de Barra do Bugres-MT, lócus deste estudo de caso etnográfico. O objetivo desta pesquisa foi compreender e discutir as práticas da educação do e no campo como possibilidade de provocar emancipação de seus sujeitos mediante as práxis educativas por meio do projeto educativo da escola. O enfoque da pesquisa visa fundamentalmente compreender, interpretar e narrar a emancipação como inédito-viável no projeto da educação do campo. Esta pesquisa é de uma abordagem qualitativa de cunho fenomenológico. À luz do pensamento freireano, pude perceber que os assentados, ao orientarem-se pelo entendimento da história assumindo a perspectiva do inédito - viável que se compõe na dialeticidade da Pedagogia no movimento, é que a escola mantém viva o pensamento de Freire nas atividades diárias. Neste aspecto observei que o MST qualifica o pensamento freireano ao contemporaneizar a resistência da luta do oprimido nas muitas marchas que a escola promove. Essa pesquisa enseja contribuição como resistência de luta aos que compartilham por essa temática e aos educadores e educandos da escola Paulo Freire.

  2. Observation of Polar Mesosphere Summer Echoes using the Northernmost MST Radar at Eureka (80 deg N)

    Science.gov (United States)

    Swarnalingam, N.; Hocking, W.; Janches, D.; Drummond, J.

    2017-01-01

    We investigate long-term Polar Mesosphere Summer Echoes (PMSEs) observations conducted by the northern most geographically located MST radar at Eureka (80 deg N, 86 deg W). While PMSEs are a well recognized summer phenomenon in the polar regions, previous calibrated studies at Resolute Bay and Eureka using 51.5 MHz and33 MHz radars respectively, showed that PMSE backscatter signal strengths are relatively weak in the polar cap sites, compared to the auroral zone sites (Swarnalingam et al., 2009b; Singer et al., 2010). Complications arise with PMSEs in which the echo strength is controlled by the electrons, which are, in turn, influenced by heavily charged ice particles as well as the variability in the D-region plasma. In recent years, PMSE experiments were conducted inside the polar cap utilizing a 51 MHz radar located at Eureka. In this paper, we investigate calibrated observations, conducted during 2009-2015. Seasonal and diurnal variations of the backscatter signal strengths are discussed and compared to previously published results from the ALOMAR radar, which is a radar of similar design located in the auroral zone at Andenes, Norway (69 deg N, 16 deg E). At Eureka, while PMSEs are present with a daily occurrence rate which is comparable to the rate observed at the auroral zone site for at least two seasons, they show a great level of inter-annual variability. The occurrence rate for the strong echoes tends to be low. Furthermore, comparison of the absolute backscatter signal strengths at these two sites clearly indicates that the PMSE backscatter signal strength at Eureka is weak. Although this difference could be caused by several factors, we investigate the intensity of the neutral air turbulence at Eureka from the measurements of the Doppler spectrum of the PMSE backscatter signals. We found that the level of the turbulence intensity at Eureka is weak relative to previously reported results from three high latitude sites.

  3. Two-step cultivation for production of astaxanthin in Chlorella zofingiensis using a patented energy-free rotating floating photobioreactor (RFP).

    Science.gov (United States)

    Zhang, Zhao; Huang, Jim Junhui; Sun, Dongzhe; Lee, Yuankun; Chen, Feng

    2017-01-01

    In the present study, high light and nitrogen starvation with glucose-fed to the culture was found efficient to induce astaxanthin accumulation in Chlorella zofingiensis. Therefore, a two-step cultivation strategy including high biomass yield fermentation and outdoor induction with an energy-free RFP was conducted. During the fermentation, the highest cell density of 98.4gL(-1) and astaxanthin yield of 73.3mgL(-1) were achieved, which were higher than those so far reported in C. zofingiensis. During the outdoor induction, astaxanthin content was further increased by 1.5-fold leading to the highest astaxanthin productivity of 5.26mgL(-1)day(-1) under an optimal dilution of 5-fold. Our work thus provided an effective two-step cultivation strategy for production of astaxanthin by C. zofingiensis.

  4. Hippo/Mst1 stimulates transcription of the proapoptotic mediator NOXA in a FoxO1-dependent manner.

    Science.gov (United States)

    Valis, Karel; Prochazka, Lubomir; Boura, Evzen; Chladova, Jaromira; Obsil, Tomas; Rohlena, Jakub; Truksa, Jaroslav; Dong, Lan-Feng; Ralph, Stephen J; Neuzil, Jiri

    2011-02-01

    The proapoptotic protein Noxa, a member of the BH3-only Bcl-2 protein family, can effectively induce apoptosis in cancer cells, although the relevant regulatory pathways have been obscure. Previous studies of the cytotoxic effects of α-tocopheryl succinate (α-TOS) on cancer cells identified a mechanism whereby α-TOS caused apoptosis requiring the Noxa-Bak axis. In the present study, ab initio analysis revealed a conserved FoxO-binding site (DBE; DAF-16 binding element) in the NOXA promoter, and specific affinity of FoxO proteins to this DBE was confirmed by fluorescence anisotropy. FoxO1 and FoxO3a proteins accumulated in the nucleus of α-TOS-treated cells, and the drug-induced specific FoxO1 association with the NOXA promoter and its activation were validated by chromatin immunoprecipitation. Using siRNA knockdown, a specific role for the FoxO1 protein in activating NOXA transcription in cancer cells was identified. Furthermore, the proapoptotic kinase Hippo/Mst1 was found to be strongly activated by α-TOS, and inhibiting Hippo/Mst1 by specific siRNA prevented phosphorylation of FoxO1 and its nuclear translocation, thereby reducing levels of NOXA transcription and apoptosis in cancer cells exposed to α-TOS. Thus, we have demonstrated that anticancer drugs, exemplified by α-TOS, induce apoptosis by a mechanism involving the Hippo/Mst1-FoxO1-Noxa pathway. We propose that activation of this pathway provides a new paradigm for developing targeted cancer treatments.

  5. Glucose uptake and growth of glucose-limited chemostat cultures of Aspergillus niger and a disruptant lacking MstA, a high-affinity glucose transporter

    DEFF Research Database (Denmark)

    Jørgensen, Thomas R; vanKuyk, Patricia A; Poulsen, Bjarne R

    2007-01-01

    This is a study of high-affinity glucose uptake in Aspergillus niger and the effect of disruption of a high-affinity monosaccharide-transporter gene, mstA. The substrate saturation constant (K(s)) of a reference strain was about 15 microM in glucose-limited chemostat culture. Disruption of mst......-affinity uptake system of A. niger. The mstA disruptant and a reference strain were cultivated in glucose-limited chemostat cultures at low, intermediate and high dilution rate (D=0.07 h(-1), 0.14 h(-1) and 0.20 h(-1)). Mycelium harvested from steady-state cultures was subjected to glucose uptake assays...

  6. Crianças de um acampamento do MST: propostas para um projeto de educação infantil Children of a settlement of MST: indications to an infantile education

    OpenAIRE

    Liana Gonçalves Pontes Sodré

    2005-01-01

    Este trabalho tem por objetivo descrever as indicações de uma amostra de 23 crianças (quatro a seis anos), de um acampamento do MST, para um espaço de educação infantil em fase de planejamento, através de desenhos e de explicações/descrições. Os dados indicam: o interesse pelo ambiente externo e natureza; sugestões de elementos de diversão e brincadeiras e atividades ou materiais pedagógicos. Cabe destacar a indicação de materiais de construção, principalmente janelas, preocupações que se jus...

  7. Crianças de um acampamento do MST: propostas para um projeto de educação infantil Children of a settlement of MST: indications to an infantile education

    Directory of Open Access Journals (Sweden)

    Liana Gonçalves Pontes Sodré

    2005-08-01

    Full Text Available Este trabalho tem por objetivo descrever as indicações de uma amostra de 23 crianças (quatro a seis anos, de um acampamento do MST, para um espaço de educação infantil em fase de planejamento, através de desenhos e de explicações/descrições. Os dados indicam: o interesse pelo ambiente externo e natureza; sugestões de elementos de diversão e brincadeiras e atividades ou materiais pedagógicos. Cabe destacar a indicação de materiais de construção, principalmente janelas, preocupações que se justificam pelas condições precárias de sobrevivência, já que as famílias vivem a 8 anos em barracos de lona. Os resultados apresentam as crianças como informantes competentes para a realidade educacional que irão freqüentar.This work aims to describe the indications of a sample of 23 children (from 4 to 6 years old, from a settlement of MST, to an infantile education, that is in the planning phase, through drawings and explanations/descriptions. The data indicate: the interest for external environment and nature; suggestions of entertainment and fun elements and pedagogic activities or materials. It is necessary to emphasize the indication of building materials, mainly windows that are preoccupation justified by the precarious conditions of survival, because the families live for 8 years in cottages of canvas. The results indicate the children are able informants in regard to the educational reality they will attend to.

  8. Quando o Movimento dos Trabalhadores Rurais Sem Terra - MST - ocupa a escola: A educação da infância em uma escola em movimento

    Directory of Open Access Journals (Sweden)

    Arenhart, Deise

    2007-12-01

    Full Text Available This text has as an objective to reflect on an educational experience that has grown within the Pedagogy of Brazil’s Landless Rural Workers Movement- MST. The goal is to build an understanding of the social movement as an educational agent and, at the same time, to highlight the experience of a school that is based on the Pedagogy of the MST. Research was conducted in a settlement of the MST, through observations and interviews with adults and children of the settlement and associated sections in the organization of the Movement. The analysis is grounded on critical studies in the fields of Education and Sociology, in particular the Sociology of Childhood. The study indicates that to be in motion is a human formative experience born out of the Pedagogy of a social movement which attempts to become a historical project. Solidarity, class conscience, child participation, cultivating of social memory, collectivism, valuing of rural culture and a recognition of human beings as historical agents are some of the practices observed in the context under study that reflect the presence of MST as educational agent. This article is written in Portugese

  9. Prospective separation and transcriptome analyses of cortical projection neurons and interneurons based on lineage tracing by Tbr2 (Eomes)-GFP/Dcx-mRFP reporters.

    Science.gov (United States)

    Liu, Jiancheng; Wu, Xiwei; Zhang, Heying; Qiu, Runxiang; Yoshikawa, Kazuaki; Lu, Qiang

    2016-06-01

    In the cerebral cortex, projection neurons and interneurons work coordinately to establish neural networks for normal cortical functions. While the specific mechanisms that control productions of projection neurons and interneurons are beginning to be revealed, a global characterization of the molecular differences between these two neuron types is crucial for a more comprehensive understanding of their developmental specifications and functions. In this study, using lineage tracing power of combining Tbr2(Eomes)-GFP and Dcx-mRFP reporter mice, we prospectively separated intermediate progenitor cell (IPC)-derived neurons (IPNs) from non-IPC-derived neurons (non-IPNs) of the embryonic cerebral cortex. Molecular characterizations revealed that IPNs and non-IPNs were enriched with projection neurons and interneurons, respectively. Expression profiling documented cell-specific genes including differentially expressed transcriptional regulators that might be involved in cellular specifications, for instance, our data found that SOX1 and SOX2, which were known for important functions in neural stem/progenitor cells, continued to be expressed by interneurons but not by projection neurons. Transcriptome analyses of cortical neurons isolated at different stages of neurogenesis revealed distinct temporal patterns of expression of genes involved in early-born or late-born neuron specification. These data present a resource useful for further investigation of the molecular regulations and functions of projection neurons and interneurons.

  10. Crianças de um acampamento do MST: propostas para um projeto de educação infantil

    OpenAIRE

    Sodré,Liana Gonçalves Pontes

    2005-01-01

    Este trabalho tem por objetivo descrever as indicações de uma amostra de 23 crianças (quatro a seis anos), de um acampamento do MST, para um espaço de educação infantil em fase de planejamento, através de desenhos e de explicações/descrições. Os dados indicam: o interesse pelo ambiente externo e natureza; sugestões de elementos de diversão e brincadeiras e atividades ou materiais pedagógicos. Cabe destacar a indicação de materiais de construção, principalmente janelas, preocupações que se jus...

  11. Primera identificación molecular del transgen de la proteína fluorescente roja (RFP en peces Cebra (Danio rerio transgénicos ornamentales introducidos en el Perú

    Directory of Open Access Journals (Sweden)

    Carlos Scotto

    2013-01-01

    Full Text Available En el presente trabajo se identificó por primera vez peces Cebra transgénicos (Danio rerio fluorescentes de color rojo, naranja y rosado introducidos al territorio peruano de acuarios locales utilizando la técnica de PCR para amplificar el transgen RFP perteneciente a la anémona marina Discosoma spp. Se encontró una expresión génica diferencial del transgen de la proteína fluorescente roja (RFP que determinaría una gradiente de bioluminiscencia para cada color entre los peces OVM analizados. Se realizó un análisis de secuencias de las dos variantes de la RFP junto con las seis variantes de la GFP de proteínas fluorescentes existentes en el Genbank que podrían ayudar a identificar rápidamente si son nuevos genes o si son nuevas variantes de éstas proteínas fluorescentes y que podrían ser utilizadas en otros OVMs hidrobiológicos a futuro. De este modo, desarrollar y optimizar las medidas de bioseguridad mediante su oportuna detección a nivel genético molecular.

  12. A trajetória da educação infantil no MST : de ciranda em ciranda aprendendo a cirandar

    OpenAIRE

    Neiva Marisa Bihain

    2001-01-01

    Esta dissertação aborda a trajetória da Educação Infantil no MST – Movimento dos Trabalhadores Rurais Sem Terra, enfocando a Ciranda Infantil como espaço próprio da criança de zero a seis anos. O texto parte de uma contextualização sobre o movimento social em questão, após enfocamos a história da Educação Infantil no MST, no que se refere ao processo desencadeado para a realização da escolha do nome Ciranda Infantil, bem como, sua organização nas diferentes instâncias do Movimento, enquanto a...

  13. OPINIÃO PÚBLICA, MÍDIA E MOVIMENTOS SOCIAIS: OS JOVENS E O MST EM TEMPOS DE SOCIEDADE EM REDE

    OpenAIRE

    Machado, Anelise Lorenzon; Pérsigo, Patrícia Milano

    2015-01-01

    O trabalho trata sobre a formação da opinião dos jovens a respeito dos movimentos sociais, mais especificamente o Movimento dos Trabalhadores Rurais Sem Terra (MST). Apresentamos a formação da esfera pública, a estruturação dos movimentos sociais e suas formas de inserção na mídia. Realizamos uma pesquisa de opinião pública numa escola de ensino médio na cidade de Ronda Alta/RS.Palavras-chave: Opinião Pública; Mídia; MST; Sociedade em Rede; La opinión pública, los medios de comunicación y mov...

  14. A atuação do pedagogo no MST: revelações de um estudo exploratório

    Directory of Open Access Journals (Sweden)

    Georgia Sobreira dos Santos Cêa

    2009-01-01

    Full Text Available In 2006, the graduate programs in Pedagogy were defined as a degree that should provide the training of performance of the professional in schools and other spaces, including social movements. This paper aims to present a curricular experience called "integrated projects", which took as its object the performance of the teacher in Brazilian landless workers' movement (MST, and involved teachers and students of the second period of the course of Pedagogy at the Universidade Federal de Alagoas (UFAL. The article reflects on the importance of treatment of the relationship between social movements in education and training of teachers. It is presented a methodological course of the study, and it is also exposed a significant learning that it offers, with emphasis on understanding the relationship between the struggle for land and education, considering the actions taken by a teacher in the MST.

  15. Relações de gênero e subjetividades no devir MST Gender relations and subjectivities in the building of Brazil's landless movement

    Directory of Open Access Journals (Sweden)

    Cristiani Bereta da Silva

    2004-04-01

    Full Text Available Os relatórios internos e as diversas e distintas publicações do e sobre o MST produzidos nos últimos 20 anos desvelam processos que permitem perceber que outras preocupações foram constituídas em meio às lutas e disputas pela conquista da terra. Essas preocupações foram mudando, adquirindo outros contornos nas idas e vindas da produção de idéias, práticas e sujeitos de um Movimento em construção. Este estudo é um exercício crítico de reflexão sobre a natureza dessas produções nas relações cotidianas, nas tentativas de construir sujeitos militantes. Busca investigar como as mudanças foram sendo construídas e de que forma foram investidas sobre as relações de trabalho, sociais, políticas e, também, afetivas de mulheres e homens, bem como de homens e homens, e de mulheres e mulheres nas dobras do MST.The internal reports of MST and the many different publications about this Movement, produced in the last 20 years, reveal the processes and the many different issues that appeared among the dispute for a piece of land. Many worries have been added to the main concern that is about the lack of land. Ideas, practices and political concerns have emerged in this movement that is still in construction. This study is a critical exercise of reflection about the nature of these productions and of the quotidian relations about the endeavours of constructing new subjects. It tries to investigate how were the changes being constructed in the work, social and political relations as well as in the affective relationships of men X women, women X women and men X men inside the MST.

  16. Integrin α2β1 inhibits MST1 kinase phosphorylation and activates Yes-associated protein oncogenic signaling in hepatocellular carcinoma.

    Science.gov (United States)

    Wong, Kwong-Fai; Liu, Angela M; Hong, Wanjin; Xu, Zhi; Luk, John M

    2016-11-22

    The Hippo pathway regulates the down-stream target Yes-associated protein (YAP) to maintain organ homeostasis, which is commonly inactivated in many types of cancers. However, how cell adhesion dysregulates the Hippo pathway activating YAP oncogene in hepatocellular carcinoma (HCC) remains unclear. Our findings demonstrate that α2β1 integrin (but not other β1 integrins) expressed in HCC cells, after binding to collagen extracellular matrix, could inhibit MST1 kinase phosphorylation and activate YAP pro-oncogenic activities. Knockdown of integrin α2 gene (ITGA2) suppressed YAP targeted gene expression in vitro. α2β1 and collagen binding resulted in suppressing Hippo signaling of mammalian sterile 20-like kinase 1 (MST1) and Large tumor suppressor homolog 1 (LATS1) with concomitant activation of YAP-mediated connective tissue growth factor (CTGF) gene expression. In vitro kinase assay showed that MST1 is an immediate downstream target of integrin α2 with S1180 residue as the critical phosphorylation site. Clinical correlational analysis using a gene expression dataset of 228 HCC tumors revealed that ITGA2 expression was significantly associated with tumor progression, and co-expression with YAP targeted genes (AXL receptor tyrosine kinase, CTGF, cyclin D1, glypican 3, insulin like growth factor 1 receptor, and SRY-box 4) correlated with survivals of HCC patients. In conclusion, α2β1 integrin activation through cellular adhesion impacts the Hippo pathway in solid tumors and modulates MST1-YAP signaling cascade. Targeting integrin α2 holds promises for treating YAP-positive HCC.

  17. A estrutura organizacional do MST: lógica política e lógica prática

    Directory of Open Access Journals (Sweden)

    Roberto de Sousa Miranda

    Full Text Available Nosso objetivo é compreender as relações entre a base do Movimento dos Trabalhadores Rurais Sem Terra (MST e suas lideranças, tomando como elemento de mediação a estrutura organizacional formalmente adotada em 2003. Para tanto, três chaves analíticas são utilizadas: Clastres, que explora a ideia de estrutura separada do corpo social; Leach, que opõe um modelo abstrato de organização às situações práticas observadas e interpretadas a partir da materialidade do comportamento dos assentados e acampados; o Neo-institucionalismo, que trata as instituições enquanto regras e normas que regulamentam a vida social. As análises foram baseadas em observações realizadas no XII Encontro Estadual do MST na Paraíba e nos assentamentos rurais José Antonio Eufrosino e Osiel Pereira, localizados no município de Campina Grande. Recusando as abordagens que tentam medir o grau de adoção dos modelos organizacionais formais pelos grupos sociais, tentamos revelar as incongruências produtoras das dinâmicas de reestruturação da organização do MST.

  18. Aspect sensitivity in the VHF radar backscatters studied using simultaneous observations of Gadanki MST radar and GPS sonde

    Directory of Open Access Journals (Sweden)

    A. K. Ghosh

    2004-11-01

    Full Text Available Simultaneous observations made on four days using the MST radar and GPS-sonde at Gadanki (13.5° N, 79.2° E, a tropical station in India, are presented to address the aspect sensitivity of radar backscatters observed at different heights. The observations show that wherever stability parameter N2 is high, vertical shear of horizontal wind is low and Richardson number (Ri is high, the aspect sensitivity is high indicating that the aspect sensitive radar backscatters are due to thermal structures in the atmosphere. Such a case can be seen very clearly in the upper troposphere and lower stratosphere. At some heights, where N2 is high, Ri is high, but shears are relatively weak, the aspect sensitivity is found to almost disappear, indicating that some amount of shear provides favorable conditions for causing aspect sensitivity. Aspect sensitivity does not occur at all where N2 is low or negative and Ri is low in spite of wind shear being either high or low, indicating that the regions are well mixed and hence turbulent. The study also shows a power difference in the symmetric beams. A case study on this aspect suggests that this asymmetry is due to the tilting of layers by the action of atmospheric waves. There is indication that these waves are generated through Kelvin-Helmholtz-instability (KHI.

  19. Regulation of PTEN/Akt pathway enhances cardiomyogenesis and attenuates adverse left ventricular remodeling following thymosin β4 Overexpressing embryonic stem cell transplantation in the infarcted heart.

    Directory of Open Access Journals (Sweden)

    Binbin Yan

    Full Text Available Thymosin β4 (Tβ4, a small G-actin sequestering peptide, mediates cell proliferation, migration, and angiogenesis. Whether embryonic stem (ES cells, overexpressing Tβ4, readily differentiate into cardiac myocytes in vitro and in vivo and enhance cardioprotection following transplantation post myocardial infarction (MI remains unknown. Accordingly, we established stable mouse ES cell lines, RFP-ESCs and Tβ4-ESCs, expressing RFP and an RFP-Tβ4 fusion protein, respectively. In vitro, the number of spontaneously beating embryoid bodies (EBs was significantly increased in Tβ4-ESCs at day 9, 12 and 15, compared with RFP-ESCs. Enhanced expression of cardiac transcriptional factors GATA-4, Mef2c and Txb6 in Tβ4-EBs, as confirmed with real time-PCR analysis, was accompanied by the increased number of EB areas stained positive for sarcomeric α-actin in Tβ4-EBs, compared with the RFP control, suggesting a significant increase in functional cardiac myocytes. Furthermore, we transplanted Tβ4-ESCs into the infarcted mouse heart and performed morphological and functional analysis 2 weeks after MI. There was a significant increase in newly formed cardiac myocytes associated with the Notch pathway, a decrease in apoptotic nuclei mediated by an increase in Akt and a decrease in levels of PTEN. Cardiac fibrosis was significantly reduced, and left ventricular function was significantly augmented in the Tβ4-ESC transplanted group, compared with controls. It is concluded that genetically modified Tβ4-ESCs, potentiates their ability to turn into cardiac myocytes in vitro as well as in vivo. Moreover, we also demonstrate that there was a significant decrease in both cardiac apoptosis and fibrosis, thus improving cardiac function in the infarcted heart.

  20. MANPRINT Handbook for RFP Development

    Science.gov (United States)

    1990-06-01

    requirement documents such as the O&O Plan, Mission Need Statement (MNS), Joint Service Operational Requirement ( JSOR ) and ROC are circulated to potential...B Abbreviations/Acronyms J JMSNS Justification for Major System New Start (Obsolete term, see MNS) JSOR Joint Service Operational Requirement L LAV

  1. Protein phosphatase 2a (PP2A binds within the oligomerization domain of striatin and regulates the phosphorylation and activation of the mammalian Ste20-Like kinase Mst3

    Directory of Open Access Journals (Sweden)

    Jones Candace A

    2011-10-01

    Full Text Available Abstract Background Striatin, a putative protein phosphatase 2A (PP2A B-type regulatory subunit, is a multi-domain scaffolding protein that has recently been linked to several diseases including cerebral cavernous malformation (CCM, which causes symptoms ranging from headaches to stroke. Striatin association with the PP2A A/C (structural subunit/catalytic subunit heterodimer alters PP2A substrate specificity, but targets and roles of striatin-associated PP2A are not known. In addition to binding the PP2A A/C heterodimer to form a PP2A holoenzyme, striatin associates with cerebral cavernous malformation 3 (CCM3 protein, the mammalian Mps one binder (MOB homolog, Mob3/phocein, the mammalian sterile 20-like (Mst kinases, Mst3, Mst4 and STK25, and several other proteins to form a large signaling complex. Little is known about the molecular architecture of the striatin complex and the regulation of these sterile 20-like kinases. Results To help define the molecular organization of striatin complexes and to determine whether Mst3 might be negatively regulated by striatin-associated PP2A, a structure-function analysis of striatin was performed. Two distinct regions of striatin are capable of stably binding directly or indirectly to Mob3--one N-terminal, including the coiled-coil domain, and another more C-terminal, including the WD-repeat domain. In addition, striatin residues 191-344 contain determinants necessary for efficient association of Mst3, Mst4, and CCM3. PP2A associates with the coiled-coil domain of striatin, but unlike Mob3 and Mst3, its binding appears to require striatin oligomerization. Deletion of the caveolin-binding domain on striatin abolishes striatin family oligomerization and PP2A binding. Point mutations in striatin that disrupt PP2A association cause hyperphosphorylation and activation of striatin-associated Mst3. Conclusions Striatin orchestrates the regulation of Mst3 by PP2A. It binds Mst3 likely as a dimer with CCM3 via

  2. Observation of Polar Mesosphere Summer Echoes using the northernmost MST radar at Eureka (80°N)

    Science.gov (United States)

    Swarnalingam, N.; Hocking, W.; Janches, D.; Drummond, J.

    2017-09-01

    We investigate long-term Polar Mesosphere Summer Echoes (PMSEs) observations conducted by the northernmost geographically located MST radar at Eureka (80°N, 86°W). While PMSEs are a well recognized summer phenomenon in the polar regions, previous calibrated studies at Resolute Bay and Eureka using 51.5 MHz and 33 MHz radars respectively, showed that PMSE backscatter signal strengths are relatively weak in the polar cap sites, compared to the auroral zone sites (Swarnalingam et al., 2009b; Singer et al., 2010). Complications arise with PMSEs in which the echo strength is controlled by the electrons, which are, in turn, influenced by heavily charged ice particles as well as the variability in the D-region plasma. In recent years, PMSE experiments were conducted inside the polar cap utilizing a 51 MHz radar located at Eureka. In this paper, we investigate calibrated observations, conducted during 2009-2015. Seasonal and diurnal variations of the backscatter signal strengths are discussed and compared to previously published results from the ALOMAR radar, which is a radar of similar design located in the auroral zone at Andenes, Norway (69°N, 16°E). At Eureka, while PMSEs are present with a daily occurrence rate which is comparable to the rate observed at the auroral zone site for at least two seasons, they show a great level of inter-annual variability. The occurrence rate for the strong echoes tends to be low. Furthermore, comparison of the absolute backscatter signal strengths at these two sites clearly indicates that the PMSE backscatter signal strength at Eureka is weak. Although this difference could be caused by several factors, we investigate the intensity of the neutral air turbulence at Eureka from the measurements of the Doppler spectrum of the PMSE backscatter signals. We found that the level of the turbulence intensity at Eureka is weak relative to previously reported results from three high latitude sites.

  3. O caminho da terra: revisitando a história do MST no Pontal do Paranapanema - SP - desde uma ótica psicopolítica Los caminos de la tierra: reconstruyendo la memoria política del MST en el Pontal do Paranapanema - São Paulo Pathways to the land: recovering a political memory of MST - Brazil's landless workers' movement in western São Paulo, Brazil

    OpenAIRE

    Alessandro Soares da Silva

    2010-01-01

    No presente artigo pretendemos recuperar aspectos psicopolíticos da luta pela terra empreendida pelo MST no Pontal do Paranapanema - SP, conhecida região de conflitos fundiários. Este trabalho resulta de uma ampla revisão bibliográfica acerca do tema feita com o intuito de compreender os processos de construção deste movimento social e de resgatar mais do que os fatos históricos vividos por ele. Resgatar essa história contribui para a construção de uma memória coletiva destes homens e mulhere...

  4. Genusnormer i omvandling – en väg till jämställdhet? : En studie om hur mäns konstruktion av maskulinitet och femininitet påverkar jämställdhetsattityder på mansdominerade arbetsplatser

    OpenAIRE

    Bark, Hanna; Gatier, Markus; Sandström, Linda

    2015-01-01

    Titel: Genusnormer i omvandling – en väg till jämställdhet? En studie om hur mäns konstruktion av maskulinitet och femininitet påverkar jämställdhetsattityder på mansdominerade arbetsplatser. Författare: Hanna Bark, Markus Gatier och Linda Sandström Handledare: Marie Aurell Institution: Managementhögskolan, Blekinge Tekniska Högskola Kurs: Kandidatarbete i Företagsekonomi, 15 högskolepoäng. Syfte: Syftet med denna studie är att öka kunskap och förståelse för hur män konstruerar genus på mansd...

  5. MST kinases monitor actin cytoskeletal integrity and signal via c-Jun N-terminal kinase stress-activated kinase to regulate p21Waf1/Cip1 stability.

    Science.gov (United States)

    Densham, Ruth M; O'Neill, Eric; Munro, June; König, Ireen; Anderson, Kurt; Kolch, Walter; Olson, Michael F

    2009-12-01

    As well as providing a structural framework, the actin cytoskeleton plays integral roles in cell death, survival, and proliferation. The disruption of the actin cytoskeleton results in the activation of the c-Jun N-terminal kinase (JNK) stress-activated protein kinase (SAPK) pathway; however, the sensor of actin integrity that couples to the JNK pathway has not been characterized in mammalian cells. We now report that the mammalian Ste20-like (MST) kinases mediate the activation of the JNK pathway in response to the disruption of the actin cytoskeleton. One consequence of actin disruption is the JNK-mediated stabilization of p21(Waf1/Cip1) (p21) via the phosphorylation of Thr57. The expression of MST1 or MST2 was sufficient to stabilize p21 in a JNK- and Thr57-dependent manner, while the stabilization of p21 by actin disruption required MST activity. These data indicate that, in addition to being components of the Salvador-Warts-Hippo tumor suppressor network and binding partners of c-Raf and the RASSF1A tumor suppressor, MST kinases serve to monitor cytoskeletal integrity and couple via the JNK SAPK pathway to the regulation of a key cell cycle regulatory protein.

  6. A luta pela terra entre o campo e a cidade: as comunas da terra do MST, sua gestação, principais atores e desafios

    OpenAIRE

    Yamila Goldfarb

    2007-01-01

    Esta pesquisa teve por objetivo analisar o processo de constituição de uma nova forma de assentamento proposta pelo MST (Movimento dos Trabalhadores Rurais Sem Terra) no estado de São Paulo, denominada Comuna da Terra, situada em áreas nas proximidades de grandes centros urbanos, buscando identificar no que ela difere de outras formas de assentamento, no sentido de sua organização interna, e qual a sua contribuição para o avanço da luta por reforma agrária e para o desenvolvimento social e ec...

  7. Da gesta????o ao resguardo: a fala social de agentes de sa??de de um assentamento do MST em Alagoas, Nordeste do Brasil

    OpenAIRE

    Siqueira, Theresa Cristina de Albuquerque

    2003-01-01

    Este ?? um estudo de caso que teve como objetivo compreender as representa????es sociais das agentes de sa??de sobre as pr??ticas referentes ao ciclo grav??dico-puerperal em um Assentamento Rural de Alagoas organizado pelo MST. Especificamente identificamos as principais concep????es das agentes de sa??de sobre os cuidados no ciclo gesta????o-parto-puerp??rio, eselecendo as representa????es sociais sobre esses cuidados, refletindo sobre os sentidos que estas representa????es t??m para as pr??...

  8. Currículo e MST: relações de poder-saber e a produção da "subjetividade lutadora

    OpenAIRE

    Vandiner Ribeiro

    2013-01-01

    Esta tese tem como objeto de investigação os currículos de duas escolas do Movimento dos Trabalhadores Rurais Sem Terra (MST): uma de um acampamento e outra de um assentamento para analisar os processos de subjetivação em funcionamento nas práticas curriculares aí inscritas. A pesquisa está inserida no campo dos estudos de currículo, com inspiração nos trabalhos dos Estudos Culturais e nos estudos de Michel Foucault. Os conceitos centrais desse empreendimento são currículo, poder, saber, pro...

  9. O fato e a notícia. A mídia impressa e o movimento dos trabalhadores rurais sem-terra (MST.

    Directory of Open Access Journals (Sweden)

    Sônia Maria Ribeiro de Souza

    2011-11-01

    Full Text Available Este texto expressa os primeiros passos rumo às investigações sobre as manifestações ideológicas e os juízos de valor veiculados pelos meios de comunicação em Presidente Prudente especificamente os jornais O Imparcial e o Oeste Notícias, tendo como referência a luta pela terra no Pontal do Paranapanema e o contexto de organização social do Movimento dos Trabalhadores Rurais Sem-Terra (MST.

  10. Human-Driven Microbiological Contamination of Benthic and Hyporheic Sediments of an Intermittent Peri-Urban River Assessed from MST and 16S rRNA Genetic Structure Analyses

    Science.gov (United States)

    Marti, Romain; Ribun, Sébastien; Aubin, Jean-Baptiste; Colinon, Céline; Petit, Stéphanie; Marjolet, Laurence; Gourmelon, Michèle; Schmitt, Laurent; Breil, Pascal; Cottet, Marylise; Cournoyer, Benoit

    2017-01-01

    Rivers are often challenged by fecal contaminations. The barrier effect of sediments against fecal bacteria was investigated through the use of a microbial source tracking (MST) toolbox, and by Next Generation Sequencing (NGS) of V5-V6 16S rRNA gene (rrs) sequences. Non-metric multi-dimensional scaling analysis of V5-V6 16S rRNA gene sequences differentiated bacteriomes according to their compartment of origin i.e., surface water against benthic and hyporheic sediments. Classification of these reads showed the most prevalent operating taxonomic units (OTU) to be allocated to Flavobacterium and Aquabacterium. Relative numbers of Gaiella, Haliangium, and Thermoleophilum OTU matched the observed differentiation of bacteriomes according to river compartments. OTU patterns were found impacted by combined sewer overflows (CSO) through an observed increase in diversity from the sewer to the hyporheic sediments. These changes appeared driven by direct transfers of bacterial contaminants from wastewaters but also by organic inputs favoring previously undetectable bacterial groups among sediments. These NGS datasets appeared more sensitive at tracking community changes than MST markers. The human-specific MST marker HF183 was strictly detected among CSO-impacted surface waters and not river bed sediments. The ruminant-specific DNA marker was more broadly distributed but intense bovine pollution was required to detect transfers from surface water to benthic and hyporheic sediments. Some OTU showed distribution patterns in line with these MST datasets such as those allocated to the Aeromonas, Acinetobacter, and Pseudomonas. Fecal indicators (Escherichia coli and total thermotolerant coliforms) were detected all over the river course but their concentrations were not correlated with MST ones. Overall, MST and NGS datasets suggested a poor colonization of river sediments by bovine and sewer bacterial contaminants. No environmental outbreak of these bacterial contaminants was

  11. Human-Driven Microbiological Contamination of Benthic and Hyporheic Sediments of an Intermittent Peri-Urban River Assessed from MST and 16S rRNA Genetic Structure Analyses.

    Science.gov (United States)

    Marti, Romain; Ribun, Sébastien; Aubin, Jean-Baptiste; Colinon, Céline; Petit, Stéphanie; Marjolet, Laurence; Gourmelon, Michèle; Schmitt, Laurent; Breil, Pascal; Cottet, Marylise; Cournoyer, Benoit

    2017-01-01

    Rivers are often challenged by fecal contaminations. The barrier effect of sediments against fecal bacteria was investigated through the use of a microbial source tracking (MST) toolbox, and by Next Generation Sequencing (NGS) of V5-V6 16S rRNA gene (rrs) sequences. Non-metric multi-dimensional scaling analysis of V5-V6 16S rRNA gene sequences differentiated bacteriomes according to their compartment of origin i.e., surface water against benthic and hyporheic sediments. Classification of these reads showed the most prevalent operating taxonomic units (OTU) to be allocated to Flavobacterium and Aquabacterium. Relative numbers of Gaiella, Haliangium, and Thermoleophilum OTU matched the observed differentiation of bacteriomes according to river compartments. OTU patterns were found impacted by combined sewer overflows (CSO) through an observed increase in diversity from the sewer to the hyporheic sediments. These changes appeared driven by direct transfers of bacterial contaminants from wastewaters but also by organic inputs favoring previously undetectable bacterial groups among sediments. These NGS datasets appeared more sensitive at tracking community changes than MST markers. The human-specific MST marker HF183 was strictly detected among CSO-impacted surface waters and not river bed sediments. The ruminant-specific DNA marker was more broadly distributed but intense bovine pollution was required to detect transfers from surface water to benthic and hyporheic sediments. Some OTU showed distribution patterns in line with these MST datasets such as those allocated to the Aeromonas, Acinetobacter, and Pseudomonas. Fecal indicators (Escherichia coli and total thermotolerant coliforms) were detected all over the river course but their concentrations were not correlated with MST ones. Overall, MST and NGS datasets suggested a poor colonization of river sediments by bovine and sewer bacterial contaminants. No environmental outbreak of these bacterial contaminants was

  12. Electrophysiological effects of kainic acid on vasopressin-enhanced green fluorescent protein and oxytocin-monomeric red fluorescent protein 1 neurones isolated from the supraoptic nucleus in transgenic rats.

    Science.gov (United States)

    Ohkubo, J; Ohbuchi, T; Yoshimura, M; Maruyama, T; Ishikura, T; Matsuura, T; Suzuki, H; Ueta, Y

    2014-01-01

    The supraoptic nucleus (SON) contains two types of magnocellular neurosecretory cells: arginine vasopressin (AVP)-producing and oxytocin (OXT)-producing cells. We recently generated and characterised two transgenic rat lines: one expressing an AVP-enhanced green fluorescent protein (eGFP) and the other expressing an OXT-monomeric red fluorescent protein 1 (mRFP1). These transgenic rats enable the visualisation of AVP or OXT neurones in the SON. In the present study, we compared the electrophysiological responses of AVP-eGFP and OXT-mRFP1 neurones to glutamic acid in SON primary cultures. Glutamate mediates fast synaptic transmission through three classes of ionotrophic receptors: the NMDA, AMPA and kainate receptors. We investigated the contributions of the three classes of ionotrophic receptors in glutamate-induced currents. Three different antagonists were used, each predominantly selective for one of the classes of ionotrophic receptor. Next, we focused on the kainate receptors (KARs). We examined the electrophysiological effects of kainic acid (KA) on AVP-eGFP and OXT-mRFP1 neurones. In current clamp mode, KA induced depolarisation and increased firing rates. These KA-induced responses were inhibited by the non-NMDA ionotrophic receptor antagonist 6-cyano-7-nitroquinoxaline-2,3(1H4H)-dione in both AVP-eGFP and OXT-mRFP1 neurones. In voltage clamp mode, the application of KA evoked inward currents in a dose-dependent manner. The KA-induced currents were significantly larger in OXT-mRFP1 neurones than in AVP-eGFP neurones. This significant difference in KA-induced currents was abolished by the GluK1-containing KAR antagonist UBP302. At high concentrations (250-500 μm), the specific GluK1-containing KAR agonist (RS)-2-amino-3-(3-hydroxy-5-tert-butylisoxazol-4-yl) propanoic acid (ATPA) induced significantly larger currents in OXT-mRFP1 neurones than in AVP-eGFP neurones. Furthermore, the difference between the AVP-eGFP and OXT-mRFP1 neurones in the ATPA currents

  13. Tgf2转座系统在转RFP基因斑马鱼上的应用%Application of the Tgf2 transposon system to RFP transgenic Danio rerio construction

    Institute of Scientific and Technical Information of China (English)

    吴芳; 叶星; 邹曙明; 张莉莉; 孙成飞; 田园园; 白俊杰

    2014-01-01

    Improving the efficiency of gene transfer and integration is a key objective in transgenic fish research. To evaluate its use for transgenic fish applications, we used the Tgf2 transposon system to construct a transgenic zebrafish line. The Tgf2 transposon donor plasmid pTgf2-EF1α-eGFP was re-constructed by replacement of the transgenic ele-ments, EF1α-eGFP with the myosin light chain 2 promoter (mylz2) and the red fluorescent protein (RFP) gene, mylz2-RFP. The recombinant Tgf2 transposon donor plasmid, pTgf2-Mylz2-RFP was intended to serve as a transposon donor plasmid that specifically expresses red fluorescent protein in muscle tissue. The donor plasmid and the Tgf2 transposase mRNA were co-injected into zebra fish fertilized eggs. A total of 972 eggs were microinjected and 803 lar-vae survived, with a positive rate of 76.6%. The red fluorescence (RFP) gene transgenic zebrafish (F0) were subse-quently cultivated and 10 sexually mature transgenic individuals were mated with wild-type zebrafish, respectively. F1 individuals that exhibited red fluorescence on their body surface were obtained from one of the mating pairs yielding an integration efficiency of 10%. The positive rate of F2 individuals, which were generated by mating the mature trans-genic F1 with the wild-type fish, was 69%. The mature RFP positive F1 individual was then mated with wild-type fish, yielding a 69%positive rate in the F2 generation. Our results demonstrate that the Tgf2 transposon improves the effi-ciency of gene transfer and integration during transgene treatments. Thus, the Tgf2 transposon can be used to construct transgenic fish and in other transgenic fish research.%提高目的基因的转植效率与可遗传效率是转基因鱼研制的关键点之一。本研究利用近年开发的金鱼转座子系统进行转基因斑马鱼的研制,探讨其在转基因鱼上应用的可行性。通过 PCR 方法,改造 Tgf2转座子供体质粒pTgf2-EF1α-eGFP,将肌球蛋白轻链2

  14. Formas cooperativas de produção em assentamentos rurais do MST (Movimento dos Trabalhadores Rurais Sem Terra: dimensões educativas

    Directory of Open Access Journals (Sweden)

    Maria Antônia de Souza

    2000-02-01

    Full Text Available O objetivo deste texto é caracterizar as formas de cooperação agrícola existentes nos assentamentos organizados pelo MST, tendo como fonte de dados os documentos secundários produzidos pelo próprio Movimento (relatórios e cadernos de formação; realizar algumas indagações sobre a experiência dos Laboratórios Organizacionais de Campo - LOC, os cursos Técnico em Administração Cooperativa - TAC e cursos de Formação Integrado à Produção - FIP, enquanto estratégias educativas e, por fim, destacar dimensões educativas presente no movimento social.

  15. High-frequency fluctuation measurements by far-infrared laser Faraday-effect polarimetry-interferometry and forward scattering system on MST

    Energy Technology Data Exchange (ETDEWEB)

    Ding, W. X., E-mail: wding@ucla.edu; Lin, L.; Brower, D. L. [Department of Physics and Astronomy, University of California Los Angeles, Los Angeles, CA 90095 (United States); Duff, J. R. [Department of Physics, University of Wisconsin-Madison, Wisconsin 53706 (United States)

    2014-11-15

    Magnetic fluctuation-induced transport driven by global tearing modes has been measured by Faraday-effect polarimetry and interferometry (phase measurements) in the MST reversed field pinch. However, the role of small-scale broadband magnetic and density turbulence in transport remains unknown. In order to investigate broadband magnetic turbulence, we plan to upgrade the existing detector system by using planar-diode fundamental waveguide mixers optimized for high sensitivity. Initial tests indicate these mixers have ×10 sensitivity improvement compared to currently employed corner-cube Schottky-diode mixers and ×5 lower noise. Compact mixer design will allow us to resolve the wavenumbers up to k ∼ 1–2 cm{sup −1} for beam width w = 1.5 cm and 15 cm{sup −1} for beam width w = 2 mm. The system can also be used to measure the scattered signal (amplitude measurement) induced by both plasma density and magnetic fluctuations.

  16. Da concepção de cooperação do MST à sua materialização no assentamento Zumbi dos Palmares

    OpenAIRE

    Lourenço, Nielson Polucena

    2014-01-01

    Esta dissertação tem como objetivo central analisar, com base na concepção de cooperação do MST, como se dá sua materialização no Assentamento Zumbi dos Palmares. Ela visa entender a importância da cooperação para a organização da produção e do trabalho no referido assentamento, bem como os fatores responsáveis pelos conflitos e contradições estabelecidos durante sua materialização, sem perder de vista a percepção do assentamento enquanto fração do território conquistado pelo campesinato. O A...

  17. High-frequency fluctuation measurements by far-infrared laser Faraday-effect polarimetry-interferometry and forward scattering system on MST.

    Science.gov (United States)

    Ding, W X; Lin, L; Duff, J R; Brower, D L

    2014-11-01

    Magnetic fluctuation-induced transport driven by global tearing modes has been measured by Faraday-effect polarimetry and interferometry (phase measurements) in the MST reversed field pinch. However, the role of small-scale broadband magnetic and density turbulence in transport remains unknown. In order to investigate broadband magnetic turbulence, we plan to upgrade the existing detector system by using planar-diode fundamental waveguide mixers optimized for high sensitivity. Initial tests indicate these mixers have ×10 sensitivity improvement compared to currently employed corner-cube Schottky-diode mixers and ×5 lower noise. Compact mixer design will allow us to resolve the wavenumbers up to k ∼ 1-2 cm(-1) for beam width w = 1.5 cm and 15 cm(-1) for beam width w = 2 mm. The system can also be used to measure the scattered signal (amplitude measurement) induced by both plasma density and magnetic fluctuations.

  18. O simbolismo do MST na marcha e na mística: espaço itinerante de formação humana

    Directory of Open Access Journals (Sweden)

    Cristine Lima Torres

    2010-07-01

    Full Text Available

    Este artigo discute a mística e a marcha dos sem-terra vinculados ao Movimento dos Trabalhadores Rurais Sem Terra (MST. Consiste em um estudo sobre a formação humana no processo da luta pela terra, buscando construir estratégias educativas não-formais, tendo como base a oralidade, que re-significam os saberes populares. Buscou-se acompanhar o trajeto das marchas do MST da Bahia, registrar a dinâmica das místicas e das marchas, analisando as possibilidades de formação durante o processo. Utilizou-se como metodologia de trabalho a pesquisa histórica e imagética e como instrumentos as entrevistas com militantes e filmagem da dinâmica organizativa que caracteriza a temática abordada. Conclui-se que processos educativos como esses são fundamentais e viabilizam a invenção de novas formas de sociabilidade, sendo a solidariedade, a valorização pela vida, formas sociais que constituem um exercício de educação para a consciência crítica e emancipatória

  19. Imatinib induces H2AX phosphorylation and apoptosis in chronic myelogenous leukemia cells in vitro via caspase-3/Mst1 pathway

    Institute of Scientific and Technical Information of China (English)

    Yan-jun ZHANG; Lian-ning DUAN; Cheng-rong LU; Yan CAO; Yuan LUO; Rong-feng BAO; Shu YAN; Mei XUE; Feng ZHU; Zhe WANG

    2012-01-01

    Aim:Histone H2AX is a novel tumor suppressor and its phosphorylation at the C terminus (Ser139 and Tyr142)is required for tumor cell apoptosis.The aim of the present study was to elucidate the mechanisms underlying imatinib-induced C-terminal phosphorylation of H2AX in chronic myelogenous leukemia cells in vitro.Methods:BCR-ABL-positive K562 cells were used.Microscopy,Western blotting and flow cytometry were used to study the signaling pathways that regulate imatinib-induced H2AX phosphorylation and the apoptotic mechanisms.Results:Treatment of K562 cells with imatinib (1-8 μmol/L)induced phosphorylation of H2AX at Ser139 and Tyr142 in time-and dose-dependent manners.In contrast,imatinib at the same concentrations did not affect H2AX acetylation at Lys 5,and the acetylated H2AX maintained a higher level in the cells.Meanwhile,imatinib (1-8 μmol/L)activated caspase-3 and its downstream mammalian STE20-like kinase 1 (Mst1),and induced apoptosis of K562 cells.The caspase-3 inhibitor Z-VAD (40 μmol/L)reduced imatinibinduced H2AX phosphorylation at Ser139 and Tyr142 and blocked imatinib-induced apoptosis of K562 cells.Imatinib (4 μmol/L)induced expression of Williams-Beuren syndrome transcription factor (WSTF),but not wild-type p53-induced phosphatase 1 (Wip1)in K562 cells.Conclusion:The caspase-3/Mst1 pathway is required for H2AX C-terminal phosphorylation at Ser139 and Tyr142 and subsequent apoptosis in Bcr-Abl-positive K562 cells induced by imatinib.

  20. Enhanced rifampicin delivery to alveolar macrophages by solid lipid nanoparticles

    Energy Technology Data Exchange (ETDEWEB)

    Chuan Junlan [West China School of Pharmacy, Sichuan University, Key Laboratory of Drug Targeting and Drug Delivery System, Ministry of Education (China); Li Yanzhen [Tianjin Institute of Pharmaceutical Research, State Key Laboratory of Drug Delivery Technology and Pharmacokinetics (China); Yang Likai; Sun Xun [West China School of Pharmacy, Sichuan University, Key Laboratory of Drug Targeting and Drug Delivery System, Ministry of Education (China); Zhang Qiang [Peking University, State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences (China); Gong Tao, E-mail: gongtaoy@126.com; Zhang Zhirong, E-mail: zrzzl@vip.sina.com [West China School of Pharmacy, Sichuan University, Key Laboratory of Drug Targeting and Drug Delivery System, Ministry of Education (China)

    2013-05-15

    The present study aimed at developing a drug delivery system targeting the densest site of tuberculosis infection, the alveolar macrophages (AMs). Rifampicin (RFP)-loaded solid lipid nanoparticles (RFP-SLNs) with an average size of 829.6 {+-} 16.1 nm were prepared by a modified lipid film hydration method. The cytotoxicity of RFP-SLNs to AMs and alveolar epithelial type II cells (AECs) was examined using MTT assays. The viability of AMs and AECs was above 80 % after treatment with RFP-SLNs, which showed low toxicity to both AMs and AECs. Confocal Laser Scanning Microscopy was employed to observe the interaction between RFP-SLNs and both AMs and AECs. After incubating the cells with RFP-SLNs for 2 h, the fluorescent intensity in AMs was more and remained longer (from 0.5 to 12 h) when compared with that in AECs (from 0.5 to 8 h). In vitro uptake characteristics of RFP-SLNs in AMs and AECs were also investigated by detection of intracellular RFP by High performance liquid chromatography. Results showed that RFP-SLNs delivered markedly higher RFP into AMs (691.7 ng/mg in cultured AMs, 662.6 ng/mg in primary AMs) than that into AECs (319.2 ng/mg in cultured AECs, 287.2 ng/mg in primary AECs). Subsequently, in vivo delivery efficiency and the selectivity of RFP-SLNs were further verified in Sprague-Dawley rats. Under pulmonary administration of RFP-SLNs, the amount of RFP in AMs was significantly higher than that in AECs at each time point. Our results demonstrated that solid lipid nanoparticles are a promising strategy for the delivery of rifampicin to alveolar macrophages selectively.

  1. Symposium of "Shaping and Forming of High Strength Steel,Titanium, and Light Metals" at 2010 MS&T Conference

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    @@ Enhancing the performance of materials under extreme conditions and promoting energy-efficient materials for manufacturing processes are two critical issues for the materials community. In many cases, the shaping and forming characteristics of these alloys establishes the suitability of a new material for a particular application. This is especially true in several key industries, such as oil and gas, automobile, and aerospace.

  2. Macrophage stimulating protein variation enhances the risk of sporadic extrahepatic cholangiocarcinoma.

    Science.gov (United States)

    Krawczyk, Marcin; Höblinger, Aksana; Mihalache, Florentina; Grünhage, Frank; Acalovschi, Monica; Lammert, Frank; Zimmer, Vincent

    2013-07-01

    Primary sclerosing cholangitis confers risk of cholangiocarcinoma. Here, we assessed the primary sclerosing cholangitis-associated variant rs3197999 in the MST1 gene, coding for RON receptor tyrosine kinase ligand macrophage stimulating protein, in a large European cholangiocarcinoma cohort. 223 cholangiocarcinoma patients including three primary sclerosing cholangitis individuals and 355 cancer- and primary sclerosing cholangitis-free controls were genotyped for MST1 rs3197999. The cancer group departed from Hardy-Weinberg equilibrium (p = 0.022) and exhibited a trend for rs3197999 [A] overrepresentation (31% vs. 26%: p = 0.10). Homozygous rs3197999 [AA] carrier status significantly increased overall (OR = 1.97; p = 0.023) and primary sclerosing cholangitis-unrelated biliary tract cancer risk (OR = 1.84; p = 0.044), relative to homozygous common allele carriers. The association was most pronounced in patients with extrahepatic tumours. This finding was robust to multivariate analysis (p < 0.05), validating the [AA] genotype as an independent cholangiocarcinoma risk factor. These results suggest that the [AA] genotype of the common MST1 variant rs3197999 enhances genetic risk of sporadic extrahepatic cholangiocarcinoma irrespective of primary sclerosing cholangitis status, presumably by modulating inflammatory responses and/or altered MSP/RON signalling. Copyright © 2013 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd. All rights reserved.

  3. Do You Really Expect Me to Get MST Care in a VA Where Everyone is Male? Innovative Delivery of Evidence-Based Psychotherapy to Women with Military Sexual Trauma

    Science.gov (United States)

    2015-08-01

    sexual trauma (MST) are at heightened risk of developing psychiatric difficulties such as post-traumatic stress disorder (PTSD). Although the Veterans...Based Psychotherapy to Women with Military Sexual Trauma PRINCIPAL INVESTIGATOR: Ronald Acierno, PhD CONTRACTING ORGANIZATION: Medical University of...a VA Where Everyone is Male? Innovative Delivery of Evidence-Based Psychotherapy to Women with Military Sexual Trauma 5a. CONTRACT NUMBER 5b

  4. MST Kinases Monitor Actin Cytoskeletal Integrity and Signal via c-Jun N-Terminal Kinase Stress-Activated Kinase To Regulate p21Waf1/Cip1 Stability

    OpenAIRE

    Densham, R. M.; E'Neill, Eric; Munro, J; et al, ...

    2009-01-01

    As well as providing a structural framework, the actin cytoskeleton plays integral roles in cell death, survival, and proliferation. The disruption of the actin cytoskeleton results in the activation of the c-Jun N-terminal kinase (JNK) stress-activated protein kinase (SAPK) pathway; however, the sensor of actin integrity that couples to the JNK pathway has not been characterized in mammalian cells. We now report that the mammalian Ste20-like (MST) kinases mediate the activation of the JNK pa...

  5. Not seeing the forest for the trees: size of the minimum spanning trees (MSTs forest and branch significance in MST-based phylogenetic analysis.

    Directory of Open Access Journals (Sweden)

    Andreia Sofia Teixeira

    Full Text Available Trees, including minimum spanning trees (MSTs, are commonly used in phylogenetic studies. But, for the research community, it may be unclear that the presented tree is just a hypothesis, chosen from among many possible alternatives. In this scenario, it is important to quantify our confidence in both the trees and the branches/edges included in such trees. In this paper, we address this problem for MSTs by introducing a new edge betweenness metric for undirected and weighted graphs. This spanning edge betweenness metric is defined as the fraction of equivalent MSTs where a given edge is present. The metric provides a per edge statistic that is similar to that of the bootstrap approach frequently used in phylogenetics to support the grouping of taxa. We provide methods for the exact computation of this metric based on the well known Kirchhoff's matrix tree theorem. Moreover, we implement and make available a module for the PHYLOViZ software and evaluate the proposed metric concerning both effectiveness and computational performance. Analysis of trees generated using multilocus sequence typing data (MLST and the goeBURST algorithm revealed that the space of possible MSTs in real data sets is extremely large. Selection of the edge to be represented using bootstrap could lead to unreliable results since alternative edges are present in the same fraction of equivalent MSTs. The choice of the MST to be presented, results from criteria implemented in the algorithm that must be based in biologically plausible models.

  6. Observations on Stratospheric-Mesospheric-Thermospheric temperatures using Indian MST radar and co-located LIDAR during Leonid Meteor Shower (LMS

    Directory of Open Access Journals (Sweden)

    R. Selvamurugan

    Full Text Available The temporal and height statistics of the occurrence of meteor trails during the Leonid meteor shower revealed the capability of the Indian MST radar to record large numbers of meteor trails. The distribution of radio meteor trails due to a Leonid meteor shower in space and time provided a unique opportunity to construct the height profiles of lower thermospheric temperatures and winds, with good time and height resolution. There was a four-fold increase in the meteor trails observed during the LMS compared to a typical non-shower day. The temperatures were found to be in excellent continuity with the temperature profiles below the radio meteor region derived from the co-located Nd-Yag LIDAR and the maximum height of the temperature profile was extended from the LIDAR to ~110 km. There are, how-ever, some significant differences between the observed profiles and the CIRA-86 model profiles. The first results on the meteor statistics and neutral temperature are presented and discussed below. 

    Key words. Atmospheric composition and structure (pres-sure, density, and temperature History of geophysics (at-mospheric sciences Meteorology and atmospheric dynamics (middle atmosphere dynamics

  7. O discurso político da agroecologia no MST: O caso do Assentamento 17 de Abril em Eldorado dos Carajás, Pará

    Directory of Open Access Journals (Sweden)

    Caetano De’ Carli

    2013-10-01

    Full Text Available O presente artigo visa debater a agroecologia enquanto discurso político. Parte‑se das pesquisas de Altieri, Nichols, Gliessman, Gúzman, Cristoffoli, entre outros autores, para evidenciar as trajetórias da agroecologia enquanto ciência e as tentativas da sua materialização em áreas da reforma agrária. Após essas perspectivas, aborda‑se a agroecologia na prática política do Movimento dos Trabalhadores Rurais Sem‑Terra, tanto no que se refere às suas experiências‑modelos como ao discurso político que assume, em contraposição com o modelo hegemônico da Revolução Verde. Por fim, debruça‑se sobre o caso do Assentamento 17 de Abril em Eldorado dos Carajás, ressaltando como esse discurso político representa uma importante estratégia do MST em relação a uma mudança de paradigma na mentalidade dos assentados e das lideranças locais do movimento, destacando ser esse um importante passo para a materialização de produções agropecuárias alternativas nas áreas de reforma agrária no Brasil.

  8. RFP for the italien satellite AGILE

    DEFF Research Database (Denmark)

    Madsen, Peter Buch; Jørgensen, John Leif; Riis, Troels

    1999-01-01

    The document descibes the ASC Star Tracker (performance, functionality, requirements etc.) to the Italian satellite AGILE.......The document descibes the ASC Star Tracker (performance, functionality, requirements etc.) to the Italian satellite AGILE....

  9. RFP for the Comet Nuclei Tour (CONTOUR)

    DEFF Research Database (Denmark)

    Jørgensen, John Leif; Madsen, Peter Buch; Betto, Maurizio

    1999-01-01

    This document describes the ASC Star Tracker (performance, functionality, requirements etc.) to The Johns Hopkins University - Applied Physics Laboratory for their Comet Nuclei TOUR (CONTOUR) Program.......This document describes the ASC Star Tracker (performance, functionality, requirements etc.) to The Johns Hopkins University - Applied Physics Laboratory for their Comet Nuclei TOUR (CONTOUR) Program....

  10. RFP for CNES micro satellite program

    DEFF Research Database (Denmark)

    Jørgensen, John Leif; Jørgensen, Finn E; Betto, Maurizio

    1999-01-01

    This document descibes the ASC STAR TRACKER (performance, functionality, requirements etc.) to the Centre National d'Etudes Spatiales (CNES) for their micro Satellites.......This document descibes the ASC STAR TRACKER (performance, functionality, requirements etc.) to the Centre National d'Etudes Spatiales (CNES) for their micro Satellites....

  11. Relações de saúde e trabalho em assentamento rural do MST na região de fronteira Brasil-Paraguai Health and work relations in a rural settlement of the MST in the Brazil-Paraguay border region

    Directory of Open Access Journals (Sweden)

    Eduardo Espíndola Fontoura Júnior

    2011-11-01

    Full Text Available Com o presente artigo, busca-se relatar e analisar as relações entre saúde e trabalho de pequenos produtores rurais do Movimento dos Trabalhadores Rurais Sem Terra (MST. Quanto à metodologia, adotou-se o aporte da investigação participativa e as bases da educação popular. O estudo realizado tornou possível uma compreensão de saúde e de trabalho mediante as narrativas dos trabalhadores e histórias relativas às lutas coletivas contra a exclusão e a violência no campo. Constataram-se importantes aspectos próprios a esse grupo populacional, como o sentido de conquista da propriedade da terra, os conflitos advindos da 'militância' e as diferenças de gênero e geração. Com base nos relatos, analisaram-se também os acidentes e os riscos relacionados à atividade de trabalho no assentamento rural, principalmente a exposição ao agrotóxico. Confirmaram-se, ainda, a solidariedade e a cooperação como valores importantes e como base de novas relações, um modo distinto de organização social coletiva do trabalho.In this article, we aim to report on and analyze the relationship between health and work of small farmers of the Landless Workers Movement (MST. So far as the methodology is concerned, we used participatory research and the basics of popular education. The study allowed for an understanding of health and work based on the workers' narratives, stories relating to collective struggles against exclusion and violence in the countryside. Important aspects related to this population group as were discovered, such as, for example, the sense of conquest of land ownership, the conflicts arising from 'activism,' and the differences of gender and generations. From the reports that were provided, we also examined the accidents and risks related to work in the rural settlement, particularly exposure to pesticides. It was also found that solidarity and cooperation are important values, basic elements for new relations and a distinctive

  12. Bauer, Carlos. Educação, terra e liberdade: princípios educacionais do MST em perspectiva histórica. São Paulo: Ediciones Pulsar - Xamã, 2009. 151 p.

    Directory of Open Access Journals (Sweden)

    Diana Crucelly González Rey

    2011-05-01

    Full Text Available El trabajo del Carlos Bauer, es producto de su tesis pos-doctoral desarrollada conjunto al núcleo de estudios sobre movimientos sociales, educación y ciudadanía (GEMDEC de la Uni-versidad Estatal de Campinas en São Paulo, Brasil. Esta edición es publicada en portugués en el año 2009, siendo la educación en el Movimiento Sin Tierra (MST su objeto principal del estudio.

  13. Oral cancer/endothelial cell fusion experiences nuclear fusion and acquisition of enhanced survival potential.

    Science.gov (United States)

    Song, Kai; Song, Yong; Zhao, Xiao-Ping; Shen, Hui; Wang, Meng; Yan, Ting-Lin; Liu, Ke; Shang, Zheng-Jun

    2014-10-15

    Most previous studies have linked cancer-macrophage fusion with tumor progression and metastasis. However, the characteristics of hybrid cells derived from oral cancer and endothelial cells and their involvement in cancer remained unknown. Double-immunofluorescent staining and fluorescent in situ hybridization (FISH) were performed to confirm spontaneous cell fusion between eGFP-labeled human umbilical vein endothelial cells (HUVECs) and RFP-labeled SCC9, and to detect the expression of vementin and cytokeratin 18 in the hybrids. The property of chemo-resistance of such hybrids was examined by TUNEL assay. The hybrid cells in xenografted tumor were identified by FISH and GFP/RFP dual-immunofluoresence staining. We showed that SCC9 cells spontaneously fused with cocultured endothelial cells, and the resultant hybrid cells maintained the division and proliferation activity after re-plating and thawing. Such hybrids expressed markers of both parental cells and became more resistant to chemotherapeutic drug cisplatin as compared to the parental SCC9 cells. Our in vivo data indicated that the hybrid cells contributed to tumor composition by using of immunostaining and FISH analysis, even though the hybrid cells and SCC9 cells were mixed with 1:10,000, according to the FACS data. Our study suggested that the fusion events between oral cancer and endothelial cells undergo nuclear fusion and acquire a new property of drug resistance and consequently enhanced survival potential. These experimental findings provide further supportive evidence for the theory that cell fusion is involved in cancer progression.

  14. A CONVIVÊNCIA DAS RACIONALIDADES CAMPONESA E ECONÔMICA NUM ASSENTAMENTO DO MST: SEMEANDO QUESTÕES SOBRE O TRABALHO

    Directory of Open Access Journals (Sweden)

    Mariana Fernandes da Cunha Loureiro Amorim

    2012-05-01

    Full Text Available O Movimento dos Trabalhadores Rurais Sem Terra (MST tem angariado diversas conquistas na consolidação de assentamentos pela reforma agrária no estado de Pernambuco. Nos assentamentos, o MST propõe uma forma de organização do trabalho peculiar, regida por lógicas diversas daquelas presentes nas agroindústrias. Isso instigou a curiosidade de conhecer qual a relação que os assentados estabelecem com o sistema produtor dominante e com a experiência dentro do assentamento. Isso permitiu compreender melhor a realidade de trabalho dos assentados rurais, em termos de representação e vivência do trabalho do assentado rural no campo, dentro do contexto do capitalismo, em contraposição ao trabalho proletário urbano ou rural (no agronegócio. Na construção do referencial teórico, considerou-se necessário, primeiramente, fazer um estudo sobre trabalho na contemporaneidade. Depois, foi enfatizada a persistência e as transformações que sofreu o campesinato no capitalismo, assim como as particularidades do trabalho do camponês em contraposição ao trabalho do proletário. Em especial, foi contrastada a racionalidade econômica e a racionalidade camponesa. A metodologia escolhida é qualitativa, uma vez que permite acesso a informações detalhadas de um pequeno número de casos e a conseqüente compreensão em profundidade de determinadas situações. Particularmente, esta pesquisa se classifica como um estudo qualitativo básico ou genérico, cuja análise dos dados tipicamente conduz a uma identificação de padrões recorrentes (na forma de categorias, fatores, variáveis, temas, mediante o emparelhamento com conceitos, modelos e teorias. Além das pesquisas bibliográficas e documental, foram realizadas entrevistas não-estruturadas e observação não-participante como métodos de coleta de dados primários. O lócus de investigação foi o assentamento Chico Mendes III, localizado na Região do Litoral Norte pernambucana. A

  15. MAARSY - the new MST radar on Andøya: first results of spaced antenna and Doppler measurements of atmospheric winds in the troposphere and mesosphere using a partial array

    Science.gov (United States)

    Stober, G.; Latteck, R.; Rapp, M.; Singer, W.; Zecha, M.

    2012-09-01

    MST radars have been used to study the troposphere, stratosphere and mesosphere over decades. These radars have proven to be a valuable tool to investigate atmospheric dynamics. MAARSY, the new MST radar at the island of Andøya uses a phased array antenna and is able to perform spaced antenna and Doppler measurements at the same time with high temporal and spatial resolution. Here we present first wind observations using the initial expansion stage during summer 2010. The tropospheric spaced antenna and Doppler beam swinging experiments are compared to radiosonde measurements, which were launched at the nearby Andøya Rocket Range (ARR). The mesospheric wind observations are evaluated versus common volume meteor radar wind measurements. The beam steering capabilities of MAARSY are demonstrated by performing systematic scans of polar mesospheric summer echoes (PMSE) using 25 and 91 beam directions. These wind observations permit to evaluate the new radar against independent measurements from radiosondes and meteor radar measurements to demonstrate its capabilities to provide reliable wind data from the troposphere up to the mesosphere.

  16. Atmospheric temp erature profiles estimated by the vertical wind sp eed observed by MST radar%基于MST雷达垂直风速的大气温度剖面反演

    Institute of Scientific and Technical Information of China (English)

    青海银; 张援农; 周晨; 赵正予; 陈罡

    2014-01-01

    本文主要利用MST(mesosphere-stratosphere-troposphere)雷达垂直风速时间序列进行频谱分析,计算B-V(Brunt-Väisälä)频率,再根据B-V频率和温度的关系,本文建立了离散的温度反演模型,最后反演得到了全高度的温度剖面。通过计算温度剖面和探空仪实测温度剖面比较可见,二者的符合度非常高,变化趋势也完全一致。本文还进一步讨论了,利用B-V频率和MST雷达的水平风速还可以计算理查德森数,通过理查德森数可以很清晰的判断大气的稳定性,进而可以量化大气很多动力学特征以及解释大气中的很多波动现象。因此, B-V频率的获得是MST雷达对大气动力学研究的又一贡献,它可以准确地反演大气的温度剖面,同时获取大气动力学稳定性参数。%Atmospheric temperature is an important parameter for studying the process of atmospheric dynamics and pho-tochemistry, and is a significant sign of the atmospheric vertical stratification structure. It is a challenge in atmo-spheric science research to obtain temperature profiles with high space-time resolution all the time. MST (mesosphere-stratosphere-troposphere) radar, which is the modern large-scale ground-based radio remote sensing equipment, can measure 3D atmospheric winds with high space-time resolution and unattended 24 hours a day. This paper uses the time series of vertical wind observed by MST radar to make spectral analysis and calculate B-V (Brunt-Väisälä) frequen-cy. Then this paper builds the discrete model of temperature inversion based on the relationship between B-V frequency and temperature. Compared with actual measurement of temperature from radiosondes, the agreement between the radiosonde profile and the profile from MST radar data is quite good. Furthermore, the Richardson number can also be obtained by using the B-V frequency and horizontal winds, which can judge the atmospheric stability, quantize many of

  17. Novel Infectivity-Enhanced Oncolytic Adenovirus with a Capsid-Incorporated Dual-Imaging Moiety for Monitoring Virotherapy in Ovarian Cancer

    Directory of Open Access Journals (Sweden)

    Kristopher J. Kimball

    2009-09-01

    Full Text Available We sought to develop a cancer-targeted, infectivity-enhanced oncolytic adenovirus that embodies a capsid-labeling fusion for non-invasive dual-modality imaging of ovarian cancer virotherapy. A functional fusion protein composed of fluorescent and nuclear imaging tags was genetically incorporated into the capsid of an infectivity-enhanced conditionally replicative adenovirus. Incorporation of herpes simplex virus thymidine kinase (HSV-tk and monomeric red fluorescent protein 1 (mRFP1 into the viral capsid and its genomic stability were verified by molecular analyses. Replication and oncolysis were evaluated in ovarian cancer cells. Fusion functionality was confirmed by in vitro gamma camera and fluorescent microscopy imaging. Comparison of tk-mRFP virus to single-modality controls revealed similar replication efficiency and oncolytic potency. Molecular fusion did not abolish enzymatic activity of HSV-tk as the virus effectively phosphorylated thymidine both ex vivo and in vitro. In vitro fluorescence imaging demonstrated a strong correlation between the intensity of fluorescent signal and cytopathic effect in infected ovarian cancer cells, suggesting that fluorescence can be used to monitor viral replication. We have in vitro validated a new infectivity-enhanced oncolytic adenovirus with a dual-imaging modality-labeled capsid, optimized for ovarian cancer virotherapy. The new agent could provide incremental gains toward climbing the barriers for achieving conditionally replicated adenovirus efficacy in human trials.

  18. TESTING OF A FULL-SCALE ROTARY MICROFILTER FOR THE ENHANCED PROCESS FOR RADIONUCLIDES REMOVAL

    Energy Technology Data Exchange (ETDEWEB)

    Herman, D; David Stefanko, D; Michael Poirier, M; Samuel Fink, S

    2009-01-01

    Savannah River National Laboratory (SRNL) researchers are investigating and developing a rotary microfilter for solid-liquid separation applications in the Department of Energy (DOE) complex. One application involves use in the Enhanced Processes for Radionuclide Removal (EPRR) at the Savannah River Site (SRS). To assess this application, the authors performed rotary filter testing with a full-scale, 25-disk unit manufactured by SpinTek Filtration with 0.5 micron filter media manufactured by Pall Corporation. The filter includes proprietary enhancements by SRNL. The most recent enhancement is replacement of the filter's main shaft seal with a John Crane Type 28LD gas-cooled seal. The feed material was SRS Tank 8F simulated sludge blended with monosodium titanate (MST). Testing examined total insoluble solids concentrations of 0.06 wt % (126 hours of testing) and 5 wt % (82 hours of testing). The following are conclusions from this testing.

  19. Tetrameric far-red fluorescent protein as a scaffold to assemble an octavalent peptide nanoprobe for enhanced tumor targeting and intracellular uptake in vivo.

    Science.gov (United States)

    Luo, Haiming; Yang, Jie; Jin, Honglin; Huang, Chuan; Fu, Jianwei; Yang, Fei; Gong, Hui; Zeng, Shaoqun; Luo, Qingming; Zhang, Zhihong

    2011-06-01

    Relatively weak tumor affinities and short retention time in vivo hinder the application of targeting peptides in tumor molecular imaging. Multivalent strategies based on various scaffolds have been utilized to improve the ability of peptide-receptor binding or extend the clearance time of peptide-based probes. Here, we use a tetrameric far-red fluorescent protein (tfRFP) as a scaffold to create a self-assembled octavalent peptide fluorescent nanoprobe (Octa-FNP) using a genetic engineering approach. The multiligand connecting, fluorophore labeling and nanostructure formation of Octa-FNP were performed in one step. In vitro studies showed Octa-FNP is a 10-nm fluorescent probe with excellent serum stability. Cellular uptake of Octa-FNP by human nasopharyngeal cancer 5-8F cells is 15-fold of tetravalent probe, ∼80-fold of monovalent probe and ∼600-fold of nulvalent tfRFP. In vivo enhanced tumor targeting and intracellular uptake of Octa-FNP were confirmed using optical imaging and Western blot analysis. It achieved extremely high contrast of Octa-FNP signal between tumor tissue and normal organs, especially seldom Octa-FNP detected in liver and spleen. Owing to easy preparation, precise structural and functional control, and multivalent effect, Octa-FNP provides a powerful tool for tumor optical molecular imaging and evaluating the targeting ability of numerous peptides in vivo.

  20. Oral cancer/endothelial cell fusion experiences nuclear fusion and acquisition of enhanced survival potential

    Energy Technology Data Exchange (ETDEWEB)

    Song, Kai [Department of Oral and Maxillofacial Surgery, The Affiliated Hospital of Qingdao University, Shandong Province (China); The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory for Oral Biomedicine Ministry of Education, Wuhan University, Wuhan (China); Song, Yong [The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory for Oral Biomedicine Ministry of Education, Wuhan University, Wuhan (China); Department of Stomatology, Liu Zhou People' s Hospital, Guangxi (China); Zhao, Xiao-Ping; Shen, Hui; Wang, Meng; Yan, Ting-lin [The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory for Oral Biomedicine Ministry of Education, Wuhan University, Wuhan (China); Liu, Ke, E-mail: liuke.1999@aliyun.com [Department of Oral and Maxillofacial-Head and Neck oncology, School and Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan 430079 (China); The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory for Oral Biomedicine Ministry of Education, Wuhan University, Wuhan (China); Shang, Zheng-jun, E-mail: shangzhengjun@hotmail.com [Department of Oral and Maxillofacial-Head and Neck oncology, School and Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan 430079 (China); The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory for Oral Biomedicine Ministry of Education, Wuhan University, Wuhan (China)

    2014-10-15

    Most previous studies have linked cancer–macrophage fusion with tumor progression and metastasis. However, the characteristics of hybrid cells derived from oral cancer and endothelial cells and their involvement in cancer remained unknown. Double-immunofluorescent staining and fluorescent in situ hybridization (FISH) were performed to confirm spontaneous cell fusion between eGFP-labeled human umbilical vein endothelial cells (HUVECs) and RFP-labeled SCC9, and to detect the expression of vementin and cytokeratin 18 in the hybrids. The property of chemo-resistance of such hybrids was examined by TUNEL assay. The hybrid cells in xenografted tumor were identified by FISH and GFP/RFP dual-immunofluoresence staining. We showed that SCC9 cells spontaneously fused with cocultured endothelial cells, and the resultant hybrid cells maintained the division and proliferation activity after re-plating and thawing. Such hybrids expressed markers of both parental cells and became more resistant to chemotherapeutic drug cisplatin as compared to the parental SCC9 cells. Our in vivo data indicated that the hybrid cells contributed to tumor composition by using of immunostaining and FISH analysis, even though the hybrid cells and SCC9 cells were mixed with 1:10,000, according to the FACS data. Our study suggested that the fusion events between oral cancer and endothelial cells undergo nuclear fusion and acquire a new property of drug resistance and consequently enhanced survival potential. These experimental findings provide further supportive evidence for the theory that cell fusion is involved in cancer progression. - Highlights: • The fusion events between oral cancer and endothelial cells undergo nuclear fusion. • The resulting hybrid cells acquire a new property of drug resistance. • The resulting hybrid cells express the markers of both parental cells (i.e. vimentin and cytokeratin 18). • The hybrid cells contribute to tumor repopulation in vivo.

  1. Procesos de ambientalización y transición agroecológica en el MST: reforma agraria popular, soberanía alimentaria y ecología política

    Directory of Open Access Journals (Sweden)

    Lucas Henrique Pinto

    2015-12-01

    Full Text Available Buscaremos analizar en el presente artículo cómo la hegemonía del proyecto neoliberal en la economía mundial y los cambios productivos del régimen capitalista, llevaron al Movimiento de los Trabajadores Rurales Sin Tierra (MST a incrementar también sus consignas, tanto por los avances oriundos de conflictos organizativos internos como también por su adecuación a las nuevas coyunturas hegemónicas. Problematizaremos estos procesos interrelacionando la nueva forma de pensar la reforma agraria con algunos puntos de la llamada cuestión ambiental, que no es según nuestra hipótesis, solamente una asimilación de un tema actualmente de moda, sino es la incorporación de un aspecto central en el debate sobre el actual estadio de las fuerzas productivas capitalistas y las externalidades producidas en los procesos de producción de mercancía. Cuestiones que problematizaremos en seguida a partir de entrevistas a miembros del Movimiento y sus impresiones sobre las dinámicas de tales procesos de ambientalización y transición agroecológica.

  2. Enhancing Collegiate Women’s Soccer Psychosocial and Performance Outcomes by Promoting Intrinsic Sources of Sport Enjoyment

    Directory of Open Access Journals (Sweden)

    Scott P. Barnicle, Damon Burton

    2016-12-01

    Full Text Available This study examined the effectiveness of an applied mental skills training (MST intervention utilizing mental skills to enhance intrinsic sources of enjoyment (ISOEs as a means of promoting self-confidence, motivational style, and athletic performance, while also decreasing trait anxiety. The intervention project was designed to increase intrinsic SOE using a systematic and individualized mental training protocol, and then examine its relationships to mental skills and soccer performance. A Division 1 collegiate women’s soccer team was randomly assigned to treatment (n = 8 and control (n = 11 groups, equally distributed by academic year, position, and pre-season coach-evaluated starters and non-starts. Results revealed that the MST intervention significantly increased intrinsic enjoyment targeted psychological and competitive outcomes, both in practice and competition within the treatment group as compared to the control group. This study’s support for the impact mental skills training may have had on ISOEs, as well as other psychosocial outcomes and athletic performance can serve to highlight a mental skill often overlooked by consultants and coaches.

  3. Vinte anos do MST: a psicologia nesta história Veinte años de lo movimiento de los trabajadores rurales sin tierra: la psicología en esta historia Landless peasants’ movement after twenty years: psychology and the movement’s history

    Directory of Open Access Journals (Sweden)

    Eliane Domingues

    2007-12-01

    Full Text Available O Movimento dos Trabalhadores Rurais Sem Terra (MST completou vinte anos em 2004. Ao longo de sua existência vem despertando o interesse de pesquisadores de diversas áreas, principalmente da Educação e da Sociologia. No entanto, ainda existem poucos trabalhos na Psicologia sobre o MST. Mapear a produção científica geral sobre o MST e analisar a produção específica da Psicologia sobre esta temática é o que se propôs esta pesquisa. Com esse objetivo, foi realizada uma busca online no banco de teses da Capes, abrangendo o período de 1987 a 2004. Todos os resumos encontrados foram impressos e categorizados. Através dos resumos foram identificadas as teses e dissertações desenvolvidas em programas de pós-graduação em Psicologia, as quais, por sua vez, foram lidas em sua totalidade, buscando-se identificar as questões orientadoras, principais resultados e conclusões das pesquisas. Destaca-se a necessidade de estudos sobre a infância, desterritorialização e sofrimento psíquico e saúde mental no campo.El movimiento de los Trabajadores Rurales Sin Tierra (MST ha cumplido veinte años en 2004. A lo largo de su existencia viene despertando el interés de los investigadores de diversas áreas, principalmente, de la Educación y Sociología. Sin embargo, aun existen pocos trabajadores en la Psicología sobre el MST. Mapear la producción científica general sobre el MST y analizar la producción específica de la Psicología, sobre esta temática es lo que se ha propuesto con esta encuesta. Con ese objetivo ha sido realizada una búsqueda online en el banco de tesis de la CAPES, abarcando el período de 1987 a 2004. Todos los resúmenes encontrados fueron impresos y puesto en categorías. A través de los resúmenes fueron identificadas las tesis y disertaciones desarrolladas en Programas de Postgrado en Psicología que, por su vez, fueron leídas en su totalidad, buscando identificar las cuestiones orientadoras, principales

  4. Tip enhancement

    CERN Document Server

    Kawata, Satoshi

    2007-01-01

    This book discusses the recent advances in the area of near-field Raman scattering, mainly focusing on tip-enhanced and surface-enhanced Raman scattering. Some of the key features covered here are the optical structuring and manipulations, single molecule sensitivity, analysis of single-walled carbon nanotubes, and analytic applications in chemistry, biology and material sciences. This book also discusses the plasmonic materials for better enhancement, and optical antennas. Further, near-field microscopy based on second harmonic generation is also discussed. Chapters have been written by some of the leading scientists in this field, who present some of their recent work in this field.·Near-field Raman scattering·Tip-enhanced Raman spectroscopy·Surface-enhanced Raman spectroscopy·Nano-photonics·Nanoanalysis of Physical, chemical and biological materials beyond the diffraction limits·Single molecule detection

  5. An Approach of Degree Constraint MST Algorithm

    Directory of Open Access Journals (Sweden)

    Sanjay Kumar Pal

    2013-08-01

    Full Text Available This paper is approaching a new technique of creating Minimal Spanning Trees based on degree constraints of a simple symmetric and connected graph G. Here we recommend a new algorithm based on the average degree sequence factor of the nodes in the graph. The time complexity of the problem is less than O(N log|E| compared to the other existing time complexity algorithms is O(|E| log|E|+C of Kruskal, which is optimum. The goal is to design an algorithm that is simple, graceful, resourceful, easy to understand, and applicable in various fields starting from constraint based network design, mobile computing to other field of science and engineering.

  6. Diffusion of magnetic field lines in a confined RFP plasma

    Energy Technology Data Exchange (ETDEWEB)

    Bazzani, A. [Bologna Univ. (Italy). Dip. di Fisica; INFN, Istituto Nazionale di Fisica Nucleare, Bologna (Italy); Lab. di Tecnologia dei Materiali, Bologna (Italy); Di Sebastiano, A. [Bologna Univ. (Italy). Dip. di Fisica; Bologna Univ. (Italy). Dip. di Matematica; Turchetti, G. [Bologna Univ. (Italy). Dip. di Fisica

    1998-12-01

    A volume-preserving symplectic map is proposed to describe the magnetic field lines when the Taylor equilibrium is perturbed in a generic way. The standard scenario is observed by varying the perturbation strength, but the statistical properties in the chaotic regions are not simple due to the presence of boundaries and remnants of invariant structures. Simpler models of volume-preserving maps are proposed. The slowly modulated standard map captures the basic topological and statistical features. The diffusion is analytically described for large perturbations in terms of correlation functions and for small perturbations using the adiabatic theory, provided that the modulation is sufficiently slow.

  7. MULTIPLE PROJECTIONS SYSTEM (MPS): USER'S MANUAL VERSION 2.0

    Science.gov (United States)

    The document is a user's manual for Multiple Projections System (MPS) Version 2.0, based on the 3% reasonable further progress (RFP) tracking system that was developed in FY92/FY93. The 3% RFP tracking system is a Windows application, and enhancements to convert the 3% RFP track...

  8. Speech enhancement

    CERN Document Server

    Benesty, Jacob; Chen, Jingdong

    2006-01-01

    We live in a noisy world! In all applications (telecommunications, hands-free communications, recording, human-machine interfaces, etc.) that require at least one microphone, the signal of interest is usually contaminated by noise and reverberation. As a result, the microphone signal has to be ""cleaned"" with digital signal processing tools before it is played out, transmitted, or stored.This book is about speech enhancement. Different well-known and state-of-the-art methods for noise reduction, with one or multiple microphones, are discussed. By speech enhancement, we mean not only noise red

  9. Speech Enhancement

    DEFF Research Database (Denmark)

    Benesty, Jacob; Jensen, Jesper Rindom; Christensen, Mads Græsbøll;

    of methods and have been introduced in somewhat different contexts. Linear filtering methods originate in stochastic processes, while subspace methods have largely been based on developments in numerical linear algebra and matrix approximation theory. This book bridges the gap between these two classes......Speech enhancement is a classical problem in signal processing, yet still largely unsolved. Two of the conventional approaches for solving this problem are linear filtering, like the classical Wiener filter, and subspace methods. These approaches have traditionally been treated as different classes...... of methods by showing how the ideas behind subspace methods can be incorporated into traditional linear filtering. In the context of subspace methods, the enhancement problem can then be seen as a classical linear filter design problem. This means that various solutions can more easily be compared...

  10. Fluorescent visualisation of the hypothalamic oxytocin neurones activated by cholecystokinin-8 in rats expressing c-fos-enhanced green fluorescent protein and oxytocin-monomeric red fluorescent protein 1 fusion transgenes.

    Science.gov (United States)

    Katoh, A; Shoguchi, K; Matsuoka, H; Yoshimura, M; Ohkubo, J-I; Matsuura, T; Maruyama, T; Ishikura, T; Aritomi, T; Fujihara, H; Hashimoto, H; Suzuki, H; Murphy, D; Ueta, Y

    2014-05-01

    The up-regulation of c-fos gene expression is widely used as a marker of neuronal activation elicited by various stimuli. Anatomically precise observation of c-fos gene products can be achieved at the RNA level by in situ hybridisation or at the protein level by immunocytochemistry. Both of these methods are time and labour intensive. We have developed a novel transgenic rat system that enables the trivial visualisation of c-fos expression using an enhanced green fluorescent protein (eGFP) tag. These rats express a transgene consisting of c-fos gene regulatory sequences that drive the expression of a c-fos-eGFP fusion protein. In c-fos-eGFP transgenic rats, robust nuclear eGFP fluorescence was observed in osmosensitive brain regions 90 min after i.p. administration of hypertonic saline. Nuclear eGFP fluorescence was also observed in the supraoptic nucleus (SON) and paraventricular nucleus (PVN) 90 min after i.p. administration of cholecystokinin (CCK)-8, which selectively activates oxytocin (OXT)-secreting neurones in the hypothalamus. In double transgenic rats that express c-fos-eGFP and an OXT-monomeric red fluorescent protein 1 (mRFP1) fusion gene, almost all mRFP1-positive neurones in the SON and PVN expressed nuclear eGFP fluorescence 90 min after i.p. administration of CCK-8. It is possible that not only a plane image, but also three-dimensional reconstruction image may identify cytoplasmic vesicles in an activated neurone at the same time.

  11. Wayside acoustic diagnosis of defective train bearings based on signal resampling and information enhancement

    Science.gov (United States)

    He, Qingbo; Wang, Jun; Hu, Fei; Kong, Fanrang

    2013-10-01

    The diagnosis of train bearing defects plays a significant role to maintain the safety of railway transport. Among various defect detection techniques, acoustic diagnosis is capable of detecting incipient defects of a train bearing as well as being suitable for wayside monitoring. However, the wayside acoustic signal will be corrupted by the Doppler effect and surrounding heavy noise. This paper proposes a solution to overcome these two difficulties in wayside acoustic diagnosis. In the solution, a dynamically resampling method is firstly presented to reduce the Doppler effect, and then an adaptive stochastic resonance (ASR) method is proposed to enhance the defective characteristic frequency automatically by the aid of noise. The resampling method is based on a frequency variation curve extracted from the time-frequency distribution (TFD) of an acoustic signal by dynamically minimizing the local cost functions. For the ASR method, the genetic algorithm is introduced to adaptively select the optimal parameter of the multiscale noise tuning (MST)-based stochastic resonance (SR) method. The proposed wayside acoustic diagnostic scheme combines signal resampling and information enhancement, and thus is expected to be effective in wayside defective bearing detection. The experimental study verifies the effectiveness of the proposed solution.

  12. EDITORIAL: Enhancing nanolithography Enhancing nanolithography

    Science.gov (United States)

    Demming, Anna

    2012-01-01

    Lithography was invented in late 18th century Bavaria by an ambitious young playwright named Alois Senefelder. Senefelder experimented with stone, wax, water and ink in the hope of finding a way of reproducing text so that he might financially gain from a wider distribution of his already successful scripts. His discovery not only facilitated the profitability of his plays, but also provided the world with an affordable printing press that would ultimately democratize the dissemination of art, knowledge and literature. Since Senefelder, experiments in lithography have continued with a range of innovations including the use of electron beams and UV that allow increasingly higher-resolution features [1, 2]. Applications for this have now breached the limits of paper printing into the realms of semiconductor and microelectronic mechanical systems technology. In this issue, researchers demonstrate a technique for fabricating periodic features in poly(3,4-ethylene dioxythiophene)-poly(styrenesulfonate) (PEDOT-PSS) [3]. Their method combines field enhancements from silica nanospheres with laser-interference lithography to provide a means of patterning a polymer that has the potential to open the market of low-end, high-volume microelectronics. Laser-interference lithography has already been used successfully in patterning. Researchers in Korea used laser-interference lithography to generate stamps for imprinting a two-dimensional photonic crystal structure into green light emitting diodes (LEDs) [4]. The imprinted patterns comprised depressions 100 nm deep and 180 nm wide with a periodicity of 295 nm. In comparison with unpatterned LEDs, the intensity of photoluminescence was enhanced by a factor of seven in the LEDs that had the photonic crystal structures imprinted in them. The potential of exploiting field enhancements around nanostructures for new technologies has also attracted a great deal of attention. Researchers in the USA and Australia have used the field

  13. Throughput Enhancement Using Multiple Antennas in OFDM-based Ad Hoc Networks under Transceiver Impairments

    CERN Document Server

    Zhao, Pengkai

    2010-01-01

    Transceiver impairments, including phase noise, residual frequency offset, and imperfect channel estimation, significantly affect the performance of Multiple-Input Multiple-Output (MIMO) system. However, these impairments are not well addressed when analyzing the throughput performance of MIMO Ad Hoc networks. In this paper, we present an analytical framework to evaluate the throughput of MIMO OFDM system under the impairments of phase noise, residual frequency offset, and imperfect channel estimation. Using this framework, we evaluate the Maximum Sum Throughput (MST) in Ad Hoc networks by optimizing the power and modulation schemes of each user. Simulations are conducted to demonstrate not only the improvement in the MST from using multiple antennas, but also the loss in the MST due to the transceiver impairments. The proposed analytical framework is further applied for the distributed implementation of MST in Ad Hoc networks, where the loss caused by impairments is also evaluated.

  14. SUPPRESSION OF LIGASE4 OR XRCC6 ACTIVITIES ENHANCES THE DNA HOMOLOGOUS RECOMBINATION EFFICIENCY IN ZEBRAFISH PRIMORDIAL GERM CELLS%抑制Ligase4或Xrcc6活性增强斑马鱼原始生殖细胞中DNA同源重组的效率

    Institute of Scientific and Technical Information of China (English)

    魏志强; 熊凤; 何牡丹; 王厚鹏; 朱作言; 孙永华

    2015-01-01

    Primordial germ cells (PGCs) give rise to gametes which transmit the genetic information to next generation, therefore PGCs provide us an ideal cell type for genetic manipulation. Homologous recombina-tion (HR) is the most efficient technique to create designed genetic modifications, however, its efficiency is rather low in vertebrates. In this study, by using zebrafish as an in vivo model, we aimed to enhance the effi-ciency of HR in zebrafish PGCs. First, we injected UAS:mRFP-nos1 construct into Tg (kop:KalTA4) embryos to label the transgenic PGCs, and we showed that screening of PGCs-specific mRFP expression led to rela-tively high-efficient germline transmission of transgene. Then we established an in vivo assay to evaluate the HR frequency in PGCs. We further revealed that suppression of the activities of DNA ligase IV (Lig4) and Xrcc6 (previously known as Ku70) could significantly increase the HR efficiency, not only at whole embryo level but also in PGCs. We proposed that the Tg(kop:KalTA4) line could be used as an effective platform for HR-mediated gene targeting.%利用斑马鱼作为体内模型,研究旨在提高斑马鱼原始生殖细胞(Primordial germ cells, PGCs)中同源重组(Homologous recombination, HR)的效率。首先,将 UAS:mRFP-nos1载体显微注射到 Tg (kop:KalTA4)转基因胚胎中标记转基因PGCs,结果表明筛选PGCs特异表达mRFP的胚胎能够相对提高转基因的生殖系传递效率。随后建立了 PGCs 中 HR 效率的评估体系,并且证明抑制 DNA ligase IV(Lig4)和Xrcc6(曾用名Ku70)的活性不但在全胚胎水平,而且在PGCs水平都能够显著提高HR的效率。研究表明Tg (kop:KalTA4)转基因品系是开展HR介导的基因打靶的一个有效平台。

  15. Enhancement in Sport, and Enhancement outside Sport.

    Science.gov (United States)

    Douglas, Thomas

    2007-12-01

    Sport is one of the first areas in which enhancement has become commonplace. It is also one of the first areas in which the use of enhancement technologies has been heavily regulated. Some have thus seen sport as a testing ground for arguments about whether to permit enhancement. However, I argue that there are fairness-based objections to enhancement in sport that do not apply as strongly in some other areas of human activity. Thus, I claim that there will often be a stronger case for permitting enhancement outside of sport than for permitting enhancement in sport. I end by considering some methodological implications of this conclusion.

  16. Netrin-1 exerts oncogenic activities through enhancing Yes-associated protein stability.

    Science.gov (United States)

    Qi, Qi; Li, Dean Y; Luo, Hongbo R; Guan, Kun-Liang; Ye, Keqiang

    2015-06-09

    Yes-associated protein (YAP), a transcription coactivator, is the major downstream effector of the Hippo pathway, which plays a critical role in organ size control and cancer development. However, how YAP is regulated by extracellular stimuli in tumorigenesis remains incompletely understood. Netrin-1, a laminin-related secreted protein, displays proto-oncogenic activity in cancers. Nonetheless, the downstream signaling mediating its oncogenic effects is not well defined. Here we show that netrin-1 via its transmembrane receptors, deleted in colorectal cancer and uncoordinated-5 homolog, up-regulates YAP expression, escalating YAP levels in the nucleus and promoting cancer cell proliferation and migration. Inactivating netrin-1, deleted in colorectal cancer, or uncoordinated-5 homolog B (UNC5B) decreases YAP protein levels, abrogating cancer cell progression by netrin-1, whereas knockdown of mammalian STE20-like protein kinase 1/2 (MST1/2) or large tumor suppressor kinase 1/2 (Lats1/2), two sets of upstream core kinases of the Hippo pathway, has no effect in blocking netrin-1-induced up-regulation of YAP. Netrin-1 stimulates phosphatase 1A to dephosphorylate YAP, which leads to decreased ubiquitination and degradation, enhancing YAP accumulation and signaling. Hence, our findings support that netrin-1 exerts oncogenic activity through YAP signaling, providing a mechanism coupling extracellular signals to the nuclear YAP oncogene.

  17. Inosine enhances axon sprouting and motor recovery after spinal cord injury.

    Directory of Open Access Journals (Sweden)

    Daniel Kim

    Full Text Available Although corticospinal tract axons cannot regenerate long distances after spinal cord injury, they are able to sprout collateral branches rostral to an injury site that can help form compensatory circuits in cases of incomplete lesions. We show here that inosine enhances the formation of compensatory circuits after a dorsal hemisection of the thoracic spinal cord in mature rats and improves coordinated limb use. Inosine is a naturally occurring metabolite of adenosine that crosses the cell membrane and, in neurons, activates Mst3b, a protein kinase that is part of a signal transduction pathway that regulates axon outgrowth. Compared to saline-treated controls, rats with dorsal hemisections that were treated with inosine showed three times as many synaptic contacts between corticospinal tract collaterals and long propriospinal interneurons that project from the cervical cord to the lumbar level. Inosine-treated rats also showed stronger serotonergic reinnervation of the lumbar cord than saline-treated controls, and performed well above controls in both open-field testing and a horizontal ladder rung-walking test. Inosine was equally effective whether delivered intracranially or intravenously, and has been shown to be safe for other indications in humans. Thus, inosine might be a useful therapeutic for improving outcome after spinal cord injury.

  18. Cystathione gamma lyase/Hydrogen Sulphide Pathway Up Regulation Enhances the Responsiveness of α1A and α1B-Adrenoreceptors in the Kidney of Rats with Left Ventricular Hypertrophy

    Science.gov (United States)

    Ahmad, Ashfaq; Sattar, Munavvar A.; Azam, Maleeha; Abdulla, Mohammed H.; Khan, Safia A.; Hashmi, Fayyaz; Abdullah, Nor A.; Johns, Edward J.

    2016-01-01

    The purpose of the present study was to investigate the interaction between H2S and NO (nitric oxide) in the kidney and to evaluate its impact on the functional contribution of α1A and α1B-adrenoreceptors subtypes mediating the renal vasoconstriction in the kidney of rats with left ventricular hypertrophy (LVH). In rats the LVH induction was by isoprenaline administration and caffeine in the drinking water together with intraperitoneal administration of H2S. The responsiveness of α1A and α1B to exogenous noradrenaline, phenylephrine and methoxaminein the absence and presence of 5-methylurapidil (5-MeU) and chloroethylclonidine (CEC) was studied. Cystathione gamma lyase (CSE), cystathione β synthase (CBS), 3-mercaptopyruvate sulphar transferase (3-MST) and endothelial nitric oxide synthase (eNOS) were quantified. There was significant up regulation of CSE and eNOS in the LVH-H2S compared to the LVH group (P<0.05). Baseline renal cortical blood perfusion (RCBP) was increased (P<0.05) in the LVH-H2S compared to the LVH group. The responsiveness of α1A-adrenergic receptors to adrenergic agonists was increased (P<0.05) after administration of low dose 5-Methylurapidil in the LVH-H2S group while α1B-adrenergic receptors responsiveness to adrenergic agonists were increased (P<0.05) by both low and high dose chloroethylclonidine in the LVH-H2S group. Treatment of LVH with H2S resulted in up-regulation of CSE/H2S, CBS, and 3-MST and eNOS/NO/cGMP pathways in the kidney. These up regulation of CSE/H2S, CBS, and 3-MST and eNOS/NO/cGMP pathways enhanced the responsiveness of α1A and α1B-adrenoreceptors subtypes to adrenergic agonists in LVH-H2S. These findings indicate an important role for H2S in modulating deranged signalling in the renal vasculature resulting from LVH development. PMID:27191852

  19. Enhancement in Sport, and Enhancement outside Sport

    OpenAIRE

    Douglas, Thomas

    2007-01-01

    Sport is one of the first areas in which enhancement has become commonplace. It is also one of the first areas in which the use of enhancement technologies has been heavily regulated. Some have thus seen sport as a testing ground for arguments about whether to permit enhancement. However, I argue that there are fairness-based objections to enhancement in sport that do not apply as strongly in some other areas of human activity. Thus, I claim that there will often be a stronger case for permit...

  20. Justice, fairness, and enhancement.

    Science.gov (United States)

    Savulescu, Julian

    2006-12-01

    This article begins by considering four traditional definitions of enhancement, then proposes a fifth, the Welfarist definition. It then considers fairness-based objections to enhancement, using the example of performance enhancement in sport. In so doing it defines sport and the values proper to it, surveys alternative theories of justice, considers the natural distribution of capabilities and disabilities, and draws a distinction between social, psychological, and biological enhancement. The article advances a new argument that justice requires enhancement.

  1. Enhanced Metastatic Recurrence Via Lymphatic Trafficking of a High-Metastatic Variant of Human Triple-Negative Breast Cancer After Surgical Resection in Orthotopic Nude Mouse Models.

    Science.gov (United States)

    Yano, Shuya; Takehara, Kiyoto; Tazawa, Hiroshi; Kishimoto, Hiroyuki; Kagawa, Shunsuke; Bouvet, Michael; Fujiwara, Toshiyoshi; Hoffman, Robert M

    2017-03-01

    We previously developed and characterized a highly invasive and metastatic triple-negative breast cancer (TNBC) variant by serial orthotopic implantation of MDA-MB-231 human breast cancer cells in nude mice. Eventually, a highly invasive and metastatic variant of human TNBC was isolated after lymph node metastases was harvested and orthotopically re-implanted into the mammary gland of nude mice for two cycles. The variant thereby isolated is highly invasive in the mammary gland and metastasized to lymph nodes in 10 of 12 mice compared to 2 of 12 of the parental cell line. In the present report, we observed that high-metastatic MDA-MB-231H-RFP cells produced significantly larger subcutaneous tumors compared with parental MDA-MB-231 cells in nude mice. Extensive lymphatic trafficking by high-metastatic MDA-MB-231 cells was also observed. High-metastatic MDA-MB-231 developed larger recurrent tumors 2 weeks after tumor resection compared with tumors that were not resected in orthotopic models. Surgical resection of the MDA-MB-231 high-metastatic variant primary tumor in orthotopic models also resulted in rapid and enhanced lymphatic trafficking of residual cancer cells and extensive lymph node and lung metastasis that did not occur in the non-surgical mice. These results suggest that surgical resection of high metastatic TNBC can greatly increase the malignancy of residual cancer. J. Cell. Biochem. 118: 559-569, 2017. © 2016 Wiley Periodicals, Inc.

  2. Magnetic reconstruction of nonaxisymmetric quasi-single-helicity configurations in the Madison Symmetric Torus

    Energy Technology Data Exchange (ETDEWEB)

    Auriemma, F; Zanca, P; Franz, P; Innocente, P; Lorenzini, R; Momo, B; Terranova, D [Consorzio RFX, Associazione EURATOM-ENEA sulla Fusione 35127 Padova (Italy); Bergerson, W F; Ding, W X; Brower, D L [Department of Physics and Astronomy, University of California, Los Angeles, CA 90095 (United States); Chapman, B E [Department of Physics, University of Wisconsin, Madison, WI 53706 (United States)

    2011-10-15

    Quasi-single-helicity (QSH) states, characterized by a magnetic spectrum dominated by the innermost resonant tearing mode, are common to all the reversed field pinch (RFP) experiments. The internal magnetic field structure produced by the dominant mode is investigated for the QSH observed in the Madison Symmetric Torus (MST) RFP in discharges with zero toroidal magnetic field at the plasma boundary. The reconstruction is based on an MHD model coupled to edge measurements of the magnetic field. The model discards pressure, which has little effect on the equilibrium magnetic profile of present RFP plasmas, but adopts a realistic toroidal geometry. The technique is the adaptation to the MST configuration of a procedure already applied in RFX-mod, but a more general radial profile for the current density is needed for an adequate reconstruction of the MST case. The emerging features are similar to those found in RFX-mod. The helical flux surfaces of the dominant mode provide, with a good degree of reliability, a basis for mapping kinetic quantities such as electron density and soft-x-ray emissivity.

  3. Oxygen-enhanced combustion

    CERN Document Server

    Baukal, Charles E

    2013-01-01

    Combustion technology has traditionally been dominated by air/fuel combustion. However, two developments have increased the significance of oxygen-enhanced combustion-new technologies that produce oxygen less expensively and the increased importance of environmental regulations. Advantages of oxygen-enhanced combustion include less pollutant emissions as well as increased energy efficiency and productivity. Oxygen-Enhanced Combustion, Second Edition compiles information about using oxygen to enhance industrial heating and melting processes. It integrates fundamental principles, applications, a

  4. 脂质体微泡对超声介导基因转染的增效作用研究%The enhanced effects of liposome microbubble under ultrasound mediated gene transfection conditions

    Institute of Scientific and Technical Information of China (English)

    陈智毅; 谢明星; 王新房; 吕清; 丁尚伟

    2009-01-01

    Objective To study the transfeetion efficiency and safety of liposome microbubble(LM)on red fluorescent protein(RFP)in vitro and in vivo under ultrasound mediated gene transfection(USMGT)conditions.Methods Plasmids containing RFP were added to cultured Hela cells followed by ultrasound (US)exposure with LM.Different concentration of LM,US intensity and exposure time were optimized.Transfection efficiency was evaluated by fluorescent microscopy and FACS.Cell viability was verified by propidium iodide assay.In transplanted tumors in vivo study,LM and plasmid(P)were injected into the nude mice followed by US exposure(P+LM+US group).Nude mice undergoing plasmid injection alone(P group),plasmid injection and US exposure(P+US group)and plasmid and LM injection(P+LM group)were used as controls.Frozen section and histological examination were conducted and RFP expression was evaluated.Results LM and US exposure significantly increased transfeetion efficiency in cultured Hela cells (P< 0.01).Transfection efficiency was the most prominent under the condition of US intensity of 1.0 W/cm2 with 6%LM,duration 3 min.No apparent cell damage was found in the all groups.In transplanted tumors,strong RFP was seen in P+LM+US group.It was significantly higher than in any other groups(P<0.0 1).No tissue damage was seen histologically.Conclusions LM could enhance USMGT effectively without causing any apparently adverse effect in vitro and in vivo.This method would be a novel,effective,safe non-viral gene transfection method and provide an alternative to current clinical gene therapy.%目的 探讨超声介导基因转染时,脂质体微泡(LM)对体内、外红色荧光蛋白基因(RFP)转染的增效作用及其安全性.方法将RFP和LM加入培养的Hela细胞后行超声辐照(US),对微泡浓度、超声强度、辐照时间进行优化研究,运用荧光显微镜、流式细胞术评估基因转染率,并对细胞损伤进行分析.在裸鼠移植瘤的体内实验中,将LM

  5. Energetic-particle-driven instabilities and induced fast-ion transport in a reversed field pinch

    Science.gov (United States)

    Lin, Liang

    2013-10-01

    Multiple bursty energetic-particle (EP) modes with fishbone-like structures are observed during 1 MW tangential neutral-beam injection into MST reversed field pinch (RFP) plasmas. The distinguishing features of the RFP, including large magnetic shear (tending to add stability) and weak toroidal magnetic field (leading to large fast ion beta and stronger drive), provide a complementary environment to tokamak and stellarator configurations for exploring basic understanding of these instabilities. Detailed measurements of the EP mode characteristics and temporal-spatial dynamics reveal their influence on fast ion transport and interaction with global tearing modes. Internal magnetic field fluctuations associated with the EP modes are directly observed for the first time by Faraday-effect polarimetry (frequency ~ 90 kHz and amplitude ~ 2 G). Simultaneously measured density fluctuations exhibit a dynamically evolving and asymmetric spatial structure that peaks near the core where fast ions reside and shifts outward as the instability evolves. Furthermore, the EP mode frequencies appear at ~k∥VA , consistent with continuum modes destabilized by strong drive. The fast-ion temporal dynamics, measured by a neutral particle analyzer, resemble a classical predator-prey relaxation oscillation. It contains a slow-growing phase arising from the beam fueling followed by a rapid drop (~ 15 %) when the EP modes peak, indicating the fluctuation-induced transport maintains a stiff fast-ion density profile. The inferred transport rate is strongly enhanced (× 2) with the onset of multiple nonlinearly-interacting EP modes. The fast ions also impact global tearing modes, reducing their amplitudes by up to 65%. This mode reduction is lessened following the EP-bursts, further evidence for fast ion redistribution that weakens the suppression mechanism. Possible tearing mode suppression mechanisms will be discussed. Work supported by US DoE.

  6. Smart Image Enhancement Process

    Science.gov (United States)

    Jobson, Daniel J. (Inventor); Rahman, Zia-ur (Inventor); Woodell, Glenn A. (Inventor)

    2012-01-01

    Contrast and lightness measures are used to first classify the image as being one of non-turbid and turbid. If turbid, the original image is enhanced to generate a first enhanced image. If non-turbid, the original image is classified in terms of a merged contrast/lightness score based on the contrast and lightness measures. The non-turbid image is enhanced to generate a second enhanced image when a poor contrast/lightness score is associated therewith. When the second enhanced image has a poor contrast/lightness score associated therewith, this image is enhanced to generate a third enhanced image. A sharpness measure is computed for one image that is selected from (i) the non-turbid image, (ii) the first enhanced image, (iii) the second enhanced image when a good contrast/lightness score is associated therewith, and (iv) the third enhanced image. If the selected image is not-sharp, it is sharpened to generate a sharpened image. The final image is selected from the selected image and the sharpened image.

  7. Enhancing human capacities

    CERN Document Server

    Savulescu, Julian; Kahane, Guy

    2011-01-01

    Enhancing Human Capacities is the first to review the very latest scientific developments in human enhancement. It is unique in its examination of the ethical and policy implications of these technologies from a broad range of perspectives. Presents a rich range of perspectives on enhancement from world leading ethicists and scientists from Europe and North America The most comprehensive volume yet on the science and ethics of human enhancement Unique in providing a detailed overview of current and expected scientific advances in this area Discusses both general conceptual and ethical issues

  8. Measurement of magnetic turbulence structure and nonlinear mode coupling of tearing fluctuations in the Madison Symmetric Torus reversed field pinch edge

    Energy Technology Data Exchange (ETDEWEB)

    Assadi, S.

    1994-01-01

    Linear and nonlinear magnetohydrodynamic (MHD) stability of current-driven modes are studied in the MST reversed field pinch. Measured low frequency (f < 35 kHz) magnetic fluctuations are consistent with the global resistive tearing instabilities predicted by 3-D MHD simulations. At frequencies above 35 kHz, the magnetic fluctuations were detected to be localized and externally resonant. Discrete dynamo events, ``sawtooth oscillations,`` have been observed in the experimental RFP plasmas. This phenomenon causes the plasma to become unstable to m = 1 tearing modes. The modes that may be important in different phases of these oscillations are identified. These results then assist in nonlinear studies and also help to interpret the spectral broadening of the measured data during a discrete dynamo event. Three-wave nonlinear coupling of spectral Fourier modes is measured in the MST by applying bispectral analysis to magnetic fluctuations measured at the plasma edge at 64 toroidal locations and 16 poloidal locations, permitting observation of coupling over 8 poloidal and 32 toroidal modes. Comparison to bispectra predicted by resistive MHD computation indicates reasonably good agreement. However, during the crash phase of the sawtooth oscillation the nonlinear coupling is strongly enhanced, concomitant with a broadened k-spectrum. During the sawtooth formation the plasma is undergoing a pure diffusive process. The dynamo only occurs during the sawtooth crash. High frequency activity prior to a sawtooth crash is caused by nonlinear frequency (small-scale) mode coupling. Growth rate and coupling coefficients of toroidal mode spectra are calculated by statistical modeling. Temporal evolution of edge toroidal mode spectra has been predicted by transfer function analysis. The driving sources of electrostatic fields are different than for the magnetic fields. The characteristics of tearing modes can be altered by external field errors and addition of impurities to the plasma.

  9. Enhanced horizontal extreme-echo speed occurrence leading to polar mesospheric summer echoes (PMSE) increase at solar-wind pressure enhancement during high-speed solar wind stream events

    Science.gov (United States)

    Lee, Y.; Kirkwood, S.; Kwak, Y.; Kim, K.; Shepherd, G. G.

    2013-12-01

    We report on horizontal extreme echo speeds (HEES, ≥ 300 ms^{-1}) observed in long-periodic polar mesospheric summer echoes (PMSE) correlated with solar-wind speed in high speed solar wind streams (HSS) events. The observations were made from VHF 52 MHz radar measurements at Esrange (67.8°N, 20.4°E) between June 1-August 8 in 2006 and 2008. The periodicities of PMSE counts and the volume reflectivity primarily occur at 7, 9 and 13.5 days possibly by the effects of HSS, while the periodicities at 4-6 days are competitively coherent between planetary waves appearing in temperature and solar-wind speed during HSS events. The peaks of both HEES occurrence rate relative to PMSE and turbulence dominantly occur at solar-wind pressure enhancement with minor peaks continued under the passage of HSS over the magnetopause, followed by PMSE peaks in 1-3 days later. This study gives the results that the precipitating high-energetic particles (> 30 keV) during HSS likely induce D-region ionization involved with the consecutive processes of HEES, turbulence and PMSE. The turbulence evolved from the HEES can be explained with the Kelvin-Helmholtz instability, which was observed in PMSE by Röttger et al. [11th International Workshop on technical and scientific aspects of MST Radar, 2006] and firstly simulated for PMSE generation by Hill et al. [Earth Planets Space, 1999]. The HEES is understood as the speed of fast moving ions, accelerated by strong electric field as Lee & Shepherd [JGR, 2010] suggested with the supersonic velocities persisting in polar mesospheric clouds (PMC) region observed at enhanced O(^1S) emission rate ( 10 kR) by WINDII/UARS satellite.

  10. A polêmica sobre os transgênicos: Monsanto vs. MST / The polemic about transgenic: Monsanto vs. MST

    Directory of Open Access Journals (Sweden)

    Anna Flora Brunelli

    2011-06-01

    Full Text Available RESUMO: Neste trabalho, adotando uma perspectiva discursiva, analisamos alguns aspectos do diálogo que se trava, no Brasil, entre o discurso pró e o anti-transgênicos, procurando evidenciar as relações dialógicas que cada um deles estabelece com outros discursos. Assim, notamos que o discurso anti-transgênicos, que polemiza abertamente com o pró-transgênicos, se constrói a partir do discurso político de esquerda e do discurso científico. Já o discurso pró-transgênicos se constrói a partir do discurso científico e polemiza veladamente com o seu outro. ABSTRACT: Based on a discursive perspective, in this paper we analyze some aspects of the dialogue between the discourse in favor of and the one against transgenics in Brazil, by showing the dialogic relations that each of them engages with other discourses. We noticed that the anti-transgenics discourse, which opposes openly the pro-transgenics trend, has been built upon both the scientific and the leftist political discourses, whereas the pro-transgenics discourse has been constructed upon the scientific discourse and covertly polemicizes with its other.

  11. Enhanced superconductivity of fullerenes

    Energy Technology Data Exchange (ETDEWEB)

    Washington, II, Aaron L.; Teprovich, Joseph A.; Zidan, Ragaiy

    2017-06-20

    Methods for enhancing characteristics of superconductive fullerenes and devices incorporating the fullerenes are disclosed. Enhancements can include increase in the critical transition temperature at a constant magnetic field; the existence of a superconducting hysteresis over a changing magnetic field; a decrease in the stabilizing magnetic field required for the onset of superconductivity; and/or an increase in the stability of superconductivity over a large magnetic field. The enhancements can be brought about by transmitting electromagnetic radiation to the superconductive fullerene such that the electromagnetic radiation impinges on the fullerene with an energy that is greater than the band gap of the fullerene.

  12. Personal Identity in Enhancement

    Directory of Open Access Journals (Sweden)

    Jana Podroužková

    2015-09-01

    Full Text Available The aim of this paper is to introduce the concept of human enhancement, its methods and its relation to personal identity. Also several approaches to personal identity will be described. Transhumanism is a special think tank supporting human enhancement through modern technologies and some of its representatives claim, that even great changes to human organisms will not affect their personal identity. I will briefly describe the most important means of human enhancment and consider the problem of personal identity for each of them separately.

  13. Enhanced Magnetic Model 2015

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Enhanced Magnetic Model (EMM) extends to degree and order 720, resolving magnetic anomalies down to 56 km wavelength. The higher resolution of the EMM results in...

  14. Technology Enhanced Learning

    NARCIS (Netherlands)

    Klemke, Roland; Specht, Marcus

    2013-01-01

    Klemke, R., & Specht, M. (2013, 26-27 September). Technology Enhanced Learning. Presentation at the fourth international conference on eLearning (eLearning 2013), Belgrade, Serbia. http://econference.metropolitan.ac.rs/

  15. Ogallala Bedrock Data Enhancement

    Data.gov (United States)

    Kansas Data Access and Support Center — This data set provides an enhanced estimate of the bedrock elevation of the Ogallala Aquifer in Kansas based on lithologic logs from a variety of sources. The data...

  16. Enhanced Magnetic Model 2010

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Enhanced Magnetic Model (EMM) extends to degree and order 720, resolving magnetic anomalies down to 56 km wavelength. The higher resolution of the EMM results in...

  17. Canonical Strangeness Enhancement

    CERN Document Server

    Sollfrank, J; Redlich, Krzysztof; Satz, Helmut

    1998-01-01

    According to recent experimental data and theoretical developments we discuss three distinct topics related to strangeness enhancement in nuclear reactions. We investigate the compatibility of multi-strange particle ratios measured in a restricted phase space with thermal model parameters extracted recently in 4pi. We study the canonical suppression as a possible reason for the observed strangeness enhancement and argue that a connection between QGP formation and the undersaturation of strangeness is not excluded.

  18. Resonance Enhanced Tunneling

    CERN Document Server

    Matsumoto, S; Matsumoto, Sh.

    2000-01-01

    Time evolution of tunneling in thermal medium is examined using the real-time semiclassical formalism previously developed. Effect of anharmonic terms in the potential well is shown to give a new mechanism of resonance enhanced tunneling. If the friction from environment is small enough, this mechanism may give a very large enhancement for the tunneling rate. The case of the asymmetric wine bottle potential is worked out in detail.

  19. Enhanced metabolite generation

    Science.gov (United States)

    Chidambaram, Devicharan [Middle Island, NY

    2012-03-27

    The present invention relates to the enhanced production of metabolites by a process whereby a carbon source is oxidized with a fermentative microbe in a compartment having a portal. An electron acceptor is added to the compartment to assist the microbe in the removal of excess electrons. The electron acceptor accepts electrons from the microbe after oxidation of the carbon source. Other transfers of electrons can take place to enhance the production of the metabolite, such as acids, biofuels or brewed beverages.

  20. E-nhance Lectures

    OpenAIRE

    Naber, Larissa; Köhle, Monika

    2006-01-01

    Ever more lecturers find themselves forced to Web-enhance their courses out of economic pressure or prestige. Universities trapped between rising student numbers and decreasing budgets are turning to e-learning as the one-stop solution, with little concern for student or teacher needs. An e-(nhanced) learning environment can only be successful if it fulfils students' and lecturers' needs alike. The student needs to be supported in various stages of learning, whereas the lecturer cannot afford...

  1. Transform image enhancement

    Science.gov (United States)

    Aghagolzadeh, Sabzali; Ersoy, Okan K.

    1992-03-01

    Blockwise transform image enhancement techniques are discussed. Previously, transform image enhancement has usually been based on the discrete Fourier transform (DFT) applied to the whole image. Two major drawbacks with the DFT are high complexity of implementation involving complex multiplications and additions, with intermediate results being complex numbers, and the creation of severe block effects if image enhancement is done blockwise. In addition, the quality of enhancement is not very satisfactory. It is shown that the best transforms for transform image coding, namely, the scrambled real discrete Fourier transform, the discrete cosine transform, and the discrete cosine-III transform, are also the best for image enhancement. Three techniques of enhancement discussed in detail are alpha- rooting, modified unsharp masking, and filtering motivated by the human visual system response (HVS). With proper modifications, it is observed that unsharp masking and HVS- motivated filtering without nonlinearities are basically equivalent. Block effects are completely removed by using an overlap-save technique in addition to the best transform.

  2. Enhancer networks revealed by correlated DNAse hypersensitivity states of enhancers.

    Science.gov (United States)

    Malin, Justin; Aniba, Mohamed Radhouane; Hannenhalli, Sridhar

    2013-08-01

    Mammalian gene expression is often regulated by distal enhancers. However, little is known about higher order functional organization of enhancers. Using ∼100 K P300-bound regions as candidate enhancers, we investigated their correlated activity across 72 cell types based on DNAse hypersensitivity. We found widespread correlated activity between enhancers, which decreases with increasing inter-enhancer genomic distance. We found that correlated enhancers tend to share common transcription factor (TF) binding motifs, and several chromatin modification enzymes preferentially interact with these TFs. Presence of shared motifs in enhancer pairs can predict correlated activity with 73% accuracy. Also, genes near correlated enhancers exhibit correlated expression and share common function. Correlated enhancers tend to be spatially proximal. Interestingly, weak enhancers tend to correlate with significantly greater numbers of other enhancers relative to strong enhancers. Furthermore, strong/weak enhancers preferentially correlate with strong/weak enhancers, respectively. We constructed enhancer networks based on shared motif and correlated activity and show significant functional enrichment in their putative target gene clusters. Overall, our analyses show extensive correlated activity among enhancers and reveal clusters of enhancers whose activities are coordinately regulated by multiple potential mechanisms involving shared TF binding, chromatin modifying enzymes and 3D chromatin structure, which ultimately co-regulate functionally linked genes.

  3. Human nature and enhancement.

    Science.gov (United States)

    Buchanan, Allen

    2009-03-01

    Appeals to the idea of human nature are frequent in the voluminous literature on the ethics of enhancing human beings through biotechnology. Two chief concerns about the impact of enhancements on human nature have been voiced. The first is that enhancement may alter or destroy human nature. The second is that if enhancement alters or destroys human nature, this will undercut our ability to ascertain the good because, for us, the good is determined by our nature. The first concern assumes that altering or destroying human nature is in itself a bad thing. The second concern assumes that human nature provides a standard without which we cannot make coherent, defensible judgments about what is good. I will argue (1) that there is nothing wrong, per se, with altering or destroying human nature, because, on a plausible understanding of what human nature is, it contains bad as well as good characteristics and there is no reason to believe that eliminating some of the bad would so imperil the good as to make the elimination of the bad impermissible, and (2) that altering or destroying human nature need not result in the loss of our ability to make judgments about the good, because we possess a conception of the good by which we can and do evaluate human nature. I will argue that appeals to human nature tend to obscure rather than illuminate the debate over the ethics of enhancement and can be eliminated in favor of more cogent considerations.

  4. Enhanced nurse call systems.

    Science.gov (United States)

    2001-04-01

    This Evaluation focuses on high-end computerized nurse call systems--what we call enhanced systems. These are highly flexible systems that incorporate microprocessor and communications technologies to expand the capabilities of the nurse call function. Enhanced systems, which vary in configuration from one installation to the next, typically consist of a basic system that provides standard nurse call functionality and a combination of additional enhancements that provide the added functionality the facility desires. In this study, we examine the features that distinguish enhanced nurse call systems from nonenhanced systems, focusing on their application and benefit to healthcare facilities. We evaluated seven systems to determine how well they help (1) improve patient care, as well as increase satisfaction with the care provided, and (2) improve caregiver efficiency, as well as increase satisfaction with the work environment. We found that all systems meet these objectives, but not all systems perform equally well for all implementations. Our ratings will help facilities identify those systems that offer the most effective features for their intended use. The study also includes a Technology Management Guide to help readers (1) determine whether they'll benefit from the capabilities offered by enhanced systems and (2) target a system for purchase and equip the system for optimum performance and cost-effective operation.

  5. Christian Self-Enhancement.

    Science.gov (United States)

    Gebauer, Jochen E; Sedikides, Constantine; Schrade, Alexandra

    2017-02-16

    People overestimate themselves in domains that are central to their self-concept. Critically, the psychological status of this "self-centrality principle" remains unclear. One view regards the principle as an inextricable part of human nature and, thus, as universal and resistant to normative pressure. A contrasting view regards the principle as liable to pressure (and subsequent modification) from self-effacement norms, thus questioning its universality. Advocates of the latter view point to Christianity's robust self-effacement norms, which they consider particularly effective in curbing self-enhancement, and ascribe Christianity an ego-quieting function. Three sets of studies examined the self-centrality principle among Christians. Studies 1A and 1B (N = 2,118) operationalized self-enhancement as better-than-average perceptions on the domains of commandments of faith (self-centrality: Christians ≫ nonbelievers) and commandments of communion (self-centrality: Christians > nonbelievers). Studies 2A-2H (N = 1,779) operationalized self-enhancement as knowledge overclaiming on the domains of Christianity (self-centrality: Christians ≫ nonbelievers), communion (self-centrality: Christians > nonbelievers), and agency (self-centrality: Christians ≈ nonbelievers). Studies 3A-3J (N = 1,956) operationalized self-enhancement as grandiose narcissism on the domains of communion (self-centrality: Christians > nonbelievers) and agency (self-centrality: Christians ≈ nonbelievers). The results converged across studies, yielding consistent evidence for Christian self-enhancement. Relative to nonbelievers, Christians self-enhanced strongly in domains central to the Christian self-concept. The results also generalized across countries with differing levels of religiosity. Christianity does not quiet the ego. The self-centrality principle is resistant to normative pressure, universal, and rooted in human nature. (PsycINFO Database Record

  6. Cavity-enhanced spectroscopies

    CERN Document Server

    van Zee, Roger

    2003-01-01

    ""Cavity-Enhanced Spectroscopy"" discusses the use of optical resonators and lasers to make sensitive spectroscopic measurements. This volume is written by the researcchers who pioneered these methods. The book reviews both the theory and practice behind these spectroscopic tools and discusses the scientific discoveries uncovered by these techniques. It begins with a chapter on the use of optical resonators for frequency stabilization of lasers, which is followed by in-depth chapters discussing cavity ring-down spectroscopy, frequency-modulated, cavity-enhanced spectroscopy, intracavity spectr

  7. Enhanced Piezoelectric Shunt Design

    Directory of Open Access Journals (Sweden)

    Chul H. Park

    2003-01-01

    Full Text Available Piezoceramic material connected to an electronic shunt branch circuit has formed a successful vibration reduction device. One drawback of the conventional electronic shunt circuit is the large inductance required when suppressing low frequency vibration modes. Also, the large internal resistance associated with this large inductance exceeds the optimal design resistance needed for low frequency vibration suppression. To solve this problem, a modified and enhanced piezoelectric shunt circuit is designed and analyzed by using mechanical-electrical analogies to present the physical interpretation. The enhanced shunt circuit developed in this paper is proved to significantly reduce the targeted vibration mode of a cantilever beam, theoretically and experimentally.

  8. Biomedical enhancements as justice.

    Science.gov (United States)

    Nam, Jeesoo

    2015-02-01

    Biomedical enhancements, the applications of medical technology to make better those who are neither ill nor deficient, have made great strides in the past few decades. Using Amartya Sen's capability approach as my framework, I argue in this article that far from being simply permissible, we have a prima facie moral obligation to use these new developments for the end goal of promoting social justice. In terms of both range and magnitude, the use of biomedical enhancements will mark a radical advance in how we compensate the most disadvantaged members of society.

  9. Coorientation Theory and Assessment of the RFP Solution to Client/Service Learner Matchmaking

    Science.gov (United States)

    Rogers, Cathy; Andrews, Valerie

    2013-01-01

    Tensions that result from varying expectations of service learners and clients/community partners are as common as the pedagogical practice of service learning in public relations courses. The matchmaking process between instructors and clients can influence expectations; however, the literature includes little guidance about the process of client…

  10. RFP to work on formation flying capabilities for spacecrafts for the GRACE project

    DEFF Research Database (Denmark)

    Riis, Troels; Thuesen, Gøsta; Kilsgaard, Søren

    1999-01-01

    The National Aeronautics and Space Agency of USA, NASA, are working on formation flying capabilities for spacecrafts, GRACE Project. IAU and JPL are developing the inter spacecraft attitude link to be used on the two spacecrafts.......The National Aeronautics and Space Agency of USA, NASA, are working on formation flying capabilities for spacecrafts, GRACE Project. IAU and JPL are developing the inter spacecraft attitude link to be used on the two spacecrafts....

  11. The pre-start training technique of marksmen-sportsmen in the Olympic exercise RFP

    Directory of Open Access Journals (Sweden)

    Petriv O. S.

    2012-11-01

    Full Text Available It is shown the features of the new method prelaunch athletes in high-speed shooting of a standard pistol at five targets, which appear simultaneously at a distance of 25 meters. It is determined that the complexity of the rapid-fire is in necessity to coordinate the exact aiming at the center of the target with a smooth touch of the trigger. The methodology proposed new materials that clarify a system of forming knowledge and skills in the shooting sports. Practical recommendations containing new, scientifically based information to improve the technical and tactical actions of athletes in high-speed shooting of a standard pistol at five targets simultaneously appear at a distance of 25 meters.

  12. Development of PCR-RFP and DNA barcoding plastic markers for yellow toadflax and Dalmatian toadflax

    Science.gov (United States)

    Andrew Boswell

    2013-01-01

    Yellow toadflax and Dalmatian toadflax are problematic invasive plant species in North America. Yellow toadflax was introduced multiple times to the United States from Europe, beginning in the late 1600s. Dalmatian toadflax has similarly been repeatedly introduced to the United States, starting in 1874. Both species are known to inhabit disturbed areas, competing for...

  13. Study of Opacity Effects on Emission Lines at EXTRAP T2R RFP

    Science.gov (United States)

    Stancalie, Viorica; Rachlew, Elisabeth

    We have investigated the influence of opacity on hydrogen (H-α and Ly-β) and Li-like oxygen emission lines from the EXTRAP T2R reversed field pinch. We used the Atomic Data Analysis System (AzDAS) based on the escape factor approximation for radiative transfer to calculate metastable and excited population densities via a collisional-radiative model. Population escape factor, emergent escape factor and modified line profiles are plotted vs. optical depth. The simulated emission line ratios in the density/temperature plane are in good agreement with experimental data for electron density and temperature measurements.

  14. 3D equilibrum reconstruction for the RFP with V3FIT

    Science.gov (United States)

    Terranova, David; Marrelli, Lionello; Hanson, James; Hirshman, Steven; Marco, Gobbin; Trevisan, Gregorio

    2012-10-01

    Helical states are routinely found in all Reversed Field Pinch experiments and their description requires a 3D equilibrium reconstruction. We present the application of the V3FIT code for the RFX-mod experiment. Magnetic and kinetic diagnostics (Te from Thomson scattering, SXR emissivity, Ne from interferometer) are used in order to properly deal with the problem of degeneracy when only external magnetic measurements are used. A sensitivity study of external measurements on the internal topological structure is also presented, showing a link between external measurements and internal profiles. Fixed-boundary equilibria can be computed by independently calculating vacuum fields. The results provide a good match with experimental data and the obtained equilibria are suitable for both transport and stability analysis.

  15. SOS-red fluorescent protein (RFP) bioassay system for monitoring of antigenotoxic activity in plant extracts.

    Science.gov (United States)

    Bartolome, Amelita; Mandap, Katheryn; David, Kevim Jhean; Sevilla, Fortunato; Villanueva, James

    2006-05-15

    An optical antigenotoxicity assay using genetically engineered red fluorescent bacteria is presented. Exposure of Escherichia coli RS4U to genotoxicants [mitomycin C (MMC), nalidixic acid (NA) and hydrogen peroxide (HP)] resulted in phenotypic red fluorescence proportional to the concentration of the inducer. Except for tannic acid (TA), co-treatment of the genotoxicant-activated bacteria with ascorbic acid (AA) and aqueous plant extracts (Mangifera indica, Psidium guajava and Syzygium cumini) afforded protection against all three genotoxicants. TA was effective in suppressing the genotoxic effect of MMC and HP. The antigenotoxic effect is seen as inhibition of the genotoxicant-triggered red fluorescence. The IC50 of the plant extracts and AA varied with the genotoxicant used. Rec assay verified the antigenotoxic activity of the plant extracts. Folin-Ciocalteu test, FeCl3 test and DPPH assay confirmed the presence of polyphenolic compounds and hydrolyzable tannins in the plant extracts and the antioxidant capacity of the plant samples.

  16. 77 FR 54877 - Request for Proposals (RFP): Farm Labor Housing Technical Assistance Grants

    Science.gov (United States)

    2012-09-06

    ... USDA--Rural Housing Service; Attention: Mirna Reyes-Bible, Finance and Loan Analyst, Multi- Family... receipt. FOR FURTHER INFORMATION CONTACT: Mirna Reyes-Bible, Finance and Loan ] Analyst, Multi-Family... corporate felony convictions and corporate federal tax delinquencies. To comply with these provisions, all...

  17. Convection enhanced delivery of carboranylporphyrins for neutron capture therapy of brain tumors.

    Science.gov (United States)

    Kawabata, Shinji; Yang, Weilian; Barth, Rolf F; Wu, Gong; Huo, Tianyao; Binns, Peter J; Riley, Kent J; Ongayi, Owendi; Gottumukkala, Vijay; Vicente, M Graça H

    2011-06-01

    Boron neutron capture therapy (BNCT) is based on the nuclear capture and fission reactions that occur when non-radioactive 10B is irradiated with low energy thermal neutrons to produce α-particles (10B[n,α] Li). Carboranylporphyrins are a class of substituted porphyrins containing multiple carborane clusters. Three of these compounds, designated H2TBP, H2TCP, and H2DCP, have been evaluated in the present study. The goals were two-fold. First, to determine their biodistribution following intracerebral (i.c.) administration by short term (30 min) convection enhanced delivery (CED) or sustained delivery over 24 h by Alzet™ osmotic pumps to F98 glioma bearing rats. Second, to determine the efficacy of H2TCP and H2TBP as boron delivery agents for BNCT in F98 glioma bearing rats. Tumor boron concentrations immediately after i.c. pump delivery were high and they remained so at 24 h. The corresponding normal brain concentrations were low and the blood and liver concentrations were undetectable. Based on these data, therapy studies were initiated at the Massachusetts Institute of Technology (MIT) Research Reactor (MITR) with H2TCP and H2TBP 24 h after CED or pump delivery. Mean survival times (MST) ± standard deviations of animals that had received H2TCP or H2TBP, followed by BNCT, were of 35 ± 4 and 44 ± 10 days, compared to 23 ± 3 and 27 ± 3 days, respectively, for untreated and irradiated controls. However, since the tumor boron concentrations of the carboranylporphyrins were 3-5× higher than intravenous (i.v.) boronophenylalanine (BPA), we had expected that the MSTs would have been greater. Histopathologic examination of brains of BNCT treated rats revealed that there were large numbers of porphyrin-laden macrophages, as well as extracellular accumulations of porphyrins, indicating that the seemingly high tumor boron concentrations did not represent the true tumor cellular uptake. Nevertheless, our data are the first to show that carboranyl porphyrins can be

  18. Teaching to Enhance Research

    Science.gov (United States)

    Harland, Tony

    2016-01-01

    In this paper, I present a conceptual argument for "teaching-led research" in which university lecturers construct courses that directly and positively influence their research, while at the same time, safeguard and enhance the student experience. A research-pedagogy for higher education considers the link between teaching and research,…

  19. Simulations of Enhanced Confinement

    Science.gov (United States)

    Dorland, W.; Kotschenreuther, M.; Liu, Q. P.; Jones, C. S.; Beer, M. A.; Hammett, G. W.

    1996-11-01

    Most existing tokamaks routinely achieve enhanced confinement regimes. Designs for new, larger tokamaks therefore are typically predicated upon reliable enhanced confinement performance. However, most enhanced confinement regimes rely (to some degree) upon sheared E×B flows to stabilize the turbulence that otherwise limits the confinement. For example, the pedestal H-mode transport barrier is typically attributed to shear stabilization [Biglari, Diamond and Terry, Phys. Fl. B, 2 1 (1990)]. Unfortunately, it is easily shown that sheared E×B stabilization of microinstabilities such as the ITG mode does not scale favorably with machine size. Here, using nonlinear gyrofluid simulations in general geometry, we attempt to quantify the confinement enhancement that can be expected from velocity shear stabilization for conventional reactor plasmas. We also consider other microinstability stabilization mechanisms(See related presentations by Beer, Kotschenreuther, Manickam, and Ramos, this conference.) (strong density peaking, Shafranov shift stabilization, dots) and unconventional reactor configurations.^2 Experimental datasets from JET, DIII-D, C-Mod and TFTR are analyzed, and ITER operation is considered.

  20. Enhanced processive cellulases

    Energy Technology Data Exchange (ETDEWEB)

    Adney, William S.; Beckham, Gregg T.; Jarvis, Eric; Himmel, Michael E.; Decker, Stephen R.; Linger, Jeffrey G.; Podkaminer, Kara; Baker, John O.; Taylor, II, Larry; Xu, Qi; Singh, Arjun

    2017-06-20

    Nucleic acid sequences encoding chimeric polypeptides that exhibit enhanced cellulase activities are disclosed herein. These nucleic acids may be expressed in hosts such as fungi, which in turn may be cultured to produce chimeric polypeptides. Also disclosed are chimeric polypeptides and their use in the degradation of cellulosic materials.

  1. Enhanced Optical Filter Design

    CERN Document Server

    Cushing, David

    2011-01-01

    This book serves as a supplement to the classic texts by Angus Macleod and Philip Baumeister, taking an intuitive approach to the enhancement of optical coating (or filter) performance. Drawing from 40 years of experience in thin film design, Cushing introduces the basics of thin films, the commonly used materials and their deposition, the major coatings and their applications, and improvement methods for each.

  2. Music Enhances Learning.

    Science.gov (United States)

    Campabello, Nicolette; De Carlo, Mary Jane; O'Neil, Jean; Vacek, Mary Jill

    An action research project implemented musical strategies to affect and enhance student recall and memory. The target population was three suburban elementary schools near a major midwestern city: (1) a kindergarten classroom contained 32-38 students; (2) a second grade classroom contained 23 students and five Individualized Education Program…

  3. Competence Enhancement Behavior Management

    Science.gov (United States)

    Farmer, Thomas W.; Goforth, Jennifer B.; Hives, Jacqueline; Aaron, Annie; Jackson, Frances; Sgammato, Adrienne

    2006-01-01

    Competence Enhancement Behavior Management is presented as a framework for supporting students with challenging behaviors in general education classrooms. This approach emphasizes classroom management and discipline strategies that (a) help to build positive relations with students, (b) communicate to students that they are important, and (c)…

  4. Enhanced Attenuation: Chlorinated Organics

    Science.gov (United States)

    2008-04-01

    three sites, and lactate, whey powder, barometric pumping, and nutrient- enhanced biosparging at one site each. Full-scale applications were reported... applicable health and safety risks and precautions with respect to particular materials, conditions, or procedures in specific applications of any technology...Consequently, ITRC recommends also consulting applicable standards, laws, regulations, suppliers of materials, and material safety data sheets for

  5. Measuring and Enhancing Creativity

    Science.gov (United States)

    Mahboub, Kamyar C.; Portillo, Margaret B.; Liu, Yinhui; Chandraratna, Susantha

    2004-01-01

    The purpose of this study was to assess ways by which creativity may be enhanced in a design-oriented course. In order to demonstrate the validity of the approach, a statistically based study was employed. Additionally, the experiment was replicated in two design-oriented fields at the University of Kentucky. These fields were civil engineering…

  6. Enhancing learning with technology

    NARCIS (Netherlands)

    Specht, Marcus; Klemke, Roland

    2013-01-01

    Specht, M., & Klemke, R. (2013, 26-27 September). Enhancing Learning with Technology. In D. Milosevic (Ed.), Proceedings of the fourth international conference on eLearning (eLearning 2013) (pp. 37-45). Belgrade Metropolitan University, Belgrade, Serbia. http://econference.metropolitan.ac.rs/

  7. Wedgelet Enhanced Appearance Models

    DEFF Research Database (Denmark)

    Darkner, Sune; Larsen, Rasmus; Stegmann, Mikkel Bille;

    2004-01-01

    . The wedgelet regression trees employed are based on triangular domains and estimated using cross validation. The wedgelet regression trees are functional descriptions of the intensity information and serve to 1) reduce noise and 2) produce a compact textural description. The wedgelet enhanced appearance model...

  8. Teaching to Enhance Research

    Science.gov (United States)

    Harland, Tony

    2016-01-01

    In this paper, I present a conceptual argument for "teaching-led research" in which university lecturers construct courses that directly and positively influence their research, while at the same time, safeguard and enhance the student experience. A research-pedagogy for higher education considers the link between teaching and research,…

  9. Piezoelectrically Enhanced Photocathodes

    Science.gov (United States)

    Beach, Robert A.; Nikzad, Shouleh; Bell, Lloyd Douglas; Strittmatter, Robert

    2011-01-01

    Doping of photocathodes with materials that have large piezoelectric coefficients has been proposed as an alternative means of increasing the desired photoemission of electrons. Treating cathode materials to increase emission of electrons is called "activation" in the art. It has been common practice to activate photocathodes by depositing thin layers of suitable metals (usually, cesium). Because cesium is unstable in air, fabrication of cesiated photocathodes and devices that contain them must be performed in sealed tubes under vacuum. It is difficult and costly to perform fabrication processes in enclosed, evacuated spaces. The proposed piezoelectrically enhanced photocathodes would have electron-emission properties similar to those of cesiated photocathodes but would be stable in air, and therefore could be fabricated more easily and at lower cost. Candidate photocathodes include nitrides of elements in column III of the periodic table . especially compounds of the general formula Al(x)Ga(1.x)N (where 0< or = x < or =.1). These compounds have high piezoelectric coefficients and are suitable for obtaining response to ultraviolet light. Fabrication of a photocathode according to the proposal would include inducement of strain in cathode layers during growth of the layers on a substrate. The strain would be induced by exploiting structural mismatches among the various constituent materials of the cathode. Because of the piezoelectric effect in this material, the strain would give rise to strong electric fields that, in turn, would give rise to a high concentration of charge near the surface. Examples of devices in which piezoelectrically enhanced photocathodes could be used include microchannel plates, electron- bombarded charge-coupled devices, image tubes, and night-vision goggles. Piezoelectrically enhanced photocathode materials could also be used in making highly efficient monolithic photodetectors. Highly efficient and stable piezoelectrically enhanced

  10. Superposition Enhanced Nested Sampling

    Directory of Open Access Journals (Sweden)

    Stefano Martiniani

    2014-08-01

    Full Text Available The theoretical analysis of many problems in physics, astronomy, and applied mathematics requires an efficient numerical exploration of multimodal parameter spaces that exhibit broken ergodicity. Monte Carlo methods are widely used to deal with these classes of problems, but such simulations suffer from a ubiquitous sampling problem: The probability of sampling a particular state is proportional to its entropic weight. Devising an algorithm capable of sampling efficiently the full phase space is a long-standing problem. Here, we report a new hybrid method for the exploration of multimodal parameter spaces exhibiting broken ergodicity. Superposition enhanced nested sampling combines the strengths of global optimization with the unbiased or athermal sampling of nested sampling, greatly enhancing its efficiency with no additional parameters. We report extensive tests of this new approach for atomic clusters that are known to have energy landscapes for which conventional sampling schemes suffer from broken ergodicity. We also introduce a novel parallelization algorithm for nested sampling.

  11. Enhanced magnetocaloric effect material

    Science.gov (United States)

    Lewis, Laura J. H.

    2006-07-18

    A magnetocaloric effect heterostructure having a core layer of a magnetostructural material with a giant magnetocaloric effect having a magnetic transition temperature equal to or greater than 150 K, and a constricting material layer coated on at least one surface of the magnetocaloric material core layer. The constricting material layer may enhance the magnetocaloric effect by restriction of volume changes of the core layer during application of a magnetic field to the heterostructure. A magnetocaloric effect heterostructure powder comprising a plurality of core particles of a magnetostructural material with a giant magnetocaloric effect having a magnetic transition temperature equal to or greater than 150 K, wherein each of the core particles is encapsulated within a coating of a constricting material is also disclosed. A method for enhancing the magnetocaloric effect within a giant magnetocaloric material including the step of coating a surface of the magnetocaloric material with a constricting material is disclosed.

  12. Enhanced oil recovery

    Energy Technology Data Exchange (ETDEWEB)

    Chakma, A.; Rafiq Islam, M.; Berruti, F.

    1991-01-01

    Some forty to sixty percent of the original oil in place typically remains trapped in the reservoir after primary and secondary recoveries. Enhanced oil recovery refers to the recovery of the residual oil by different techniques. Many of the existing and proposed enhanced oil recovery techniques require sound understanding of basic chemical engineering principles such as fluid flow, heat and mass transfer, interfacial phenomena etc. Chemical EOR techniques require a good understanding of interfacial phenomena, chemical reaction, multicomponent absorption. Understanding of the fundamentals of the various EOR processes is not adequate. EOR is increasingly attracting a growing number of chemical engineers and, as a result, some of the fundamental aspect of EOR are now being investigated. However, much more remains to be done and chemical engineers can play an important role in providing a better understanding of EOR fundamentals. This volume presents selected papers on EOR presented at AICHE meetings.

  13. Vibration enhanced quantum transport

    CERN Document Server

    Semião, F L; Milburn, G J

    2009-01-01

    In this paper, we study the role of a collective vibrational motion in the phenomenon of electronic energy transfer (EET) between chromophores with different electronic transition frequencies. Previous experimental work on EET in conjugated polymer samples has suggested that the common structural framework of the macromolecule introduce correlations in the energy gap fluctuations which cause coherent EET. We present a simple model describing the coupling between the chromophores and a common vibrational mode, and find that vibration can indeed lead to an enhancement in the transport of excitations across the quantum network. Furthermore, in our model phase information is partially retained in the transfer process from a donor to an acceptor, as experimentally demonstrated in the conjugated polymer system. Consequently, this mechanism of vibration enhanced quantum transport might find applications in quantum information transfer of qubit states or entanglement.

  14. Coherence-Enhanced Spaser

    CERN Document Server

    Dorfman, Konstantin E; Voronine, Dmitri V; Genevet, Patrice; Capasso, Federico; Scully, Marlan O

    2012-01-01

    We investigate surface plasmon amplification in a silver nanoshell coupled to an externally driven three-level gain medium, and show that quantum coherence significantly enhances the generation of surface plasmons. Surface plasmon amplification by stimulated emission of radiation is achieved in the absence of population inversion on the spasing transition, which reduces the pump requirements. The coherent drive allows us to control the dynamics, and holds promise for quantum control of nanoplasmonic devices.

  15. Light enhances learned fear.

    Science.gov (United States)

    Warthen, Daniel M; Wiltgen, Brian J; Provencio, Ignacio

    2011-08-16

    The ability to learn, remember, and respond to emotional events is a powerful survival strategy. However, dysregulated behavioral and physiological responses to these memories are maladaptive. To fully understand learned fear and the pathologies that arise during response malfunction we must reveal the environmental variables that influence learned fear responses. Light, a ubiquitous environmental feature, modulates cognition and anxiety. We hypothesized that light modulates responses to learned fear. Using tone-cued fear conditioning, we found that light enhances behavioral responses to learned fear in C57BL/6J mice. Mice in light freeze more in response to a conditioned cue than mice in darkness. The absence of significant freezing during a 2-wk habituation period and during intertrial intervals indicated that light specifically modulates freezing to the learned acoustic cue rather than the context of the experimental chamber. Repeating our assay in two photoreceptor mutant models, Pde6b(rd1/rd1) and Opn4(-/-) mice, revealed that light-dependent enhancement of conditioned fear is driven primarily by the rods and/or cones. By repeating our protocol with an altered lighting regimen, we found that lighting conditions acutely modulate responses when altered between conditioning and testing. This is manifested either as an enhancement of freezing when light is added during testing or as a depression of freezing when light is removed during testing. Acute enhancement, but not depression, requires both rod/cone- and melanopsin-dependent photoreception. Our results demonstrate a modulation by light of behavioral responses to learned fear.

  16. Beyond enhanced recovery?

    DEFF Research Database (Denmark)

    Jørgensen, Christoffer C; Kehlet, Henrik

    2014-01-01

    We read with great interest the special article by Smart and Daniels discussing several important topics of perioperative care, especially regarding lack of consensus on the definition of "postoperative recovery", and need for further understanding of the pathogenic mechanisms. However, we disagr...... that a "new, clearly defined standard of what "recovery" actually is", demands "a look beyond enhanced recovery"(1) for several reasons. This article is protected by copyright. All rights reserved....

  17. Sleep for cognitive enhancement

    Directory of Open Access Journals (Sweden)

    Susanne eDiekelmann

    2014-04-01

    Full Text Available Sleep is essential for effective cognitive functioning. Loosing even a few hours of sleep can have detrimental effects on a wide variety of cognitive processes such as attention, language, reasoning, decision making, learning and memory. While sleep is necessary to ensure normal healthy cognitive functioning, it can also enhance performance beyond the boundaries of the normal condition. This article discusses the enhancing potential of sleep, mainly focusing on the domain of learning and memory. Sleep is known to facilitate the consolidation of memories learned before sleep as well as the acquisition of new memories to be learned after sleep. According to a widely held model this beneficial effect of sleep relies on the neuronal reactivation of memories during sleep that is associated with sleep-specific brain oscillations (slow oscillations, spindles, ripples as well as a characteristic neurotransmitter milieu. Recent research indicates that memory processing during sleep can be boosted by (i cueing memory reactivation during sleep, (ii stimulating sleep-specific brain oscillations, and (iii targeting specific neurotransmitter systems pharmacologically. Olfactory and auditory cues can be used, for example, to increase reactivation of associated memories during post-learning sleep. Intensifying neocortical slow oscillations (the hallmark of slow wave sleep by electrical or auditory stimulation and modulating specific neurotransmitters such as noradrenaline and glutamate likewise facilitates memory processing during sleep. With this evidence in mind, this article concludes by discussing different methodological caveats and ethical issues that should be considered when thinking about using sleep for cognitive enhancement in everyday applications.

  18. Enhanced airglow at Titan

    Science.gov (United States)

    Royer, Emilie; Esposito, Larry; Wahlund, Jan-Erik

    2016-06-01

    The Cassini Ultraviolet Imaging Spectrograph (UVIS) instrument made thousand of observations of Titan since its arrival in the Saturnian system in 2004, but only few of them have been analyzed yet. Using the imaging capability of UVIS combined to a big data analytics approach, we have been able to uncover an unexpected pattern in this observations: on several occasions the Titan airglow exhibits an enhanced brightness by approximately a factor of 2, generally combined with a lower altitude of the airglow emission peak. These events typically last from 10 to 30 minutes and are followed and preceded by an airglow of regular and expected level of brightness and altitude. Observations made by the Cassini Plasma Spectrometer (CAPS) instrument onboard Cassini allowed us to correlate the enhanced airglow observed on T-32 with an electron burst. The timing of the burst and the level of energetic electrons (1 keV) observed by CAPS correspond to a brighter and lower than typical airglow displayed on the UVIS data. Furthermore, during T-32 Titan was inside the Saturn's magnetosheath and thus more subject to bombardment by energetic particles. However, our analysis demonstrates that the presence of Titan inside the magnetosheath is not a necessary condition for the production of an enhanced airglow, as we detected other similar events while Titan was within Saturn's magnetosphere. The study presented here aims to a better understanding of the interactions of Titan's upper atmosphere with its direct environment.

  19. Structural comparison of human mammalian ste20-like kinases.

    Directory of Open Access Journals (Sweden)

    Christopher J Record

    Full Text Available BACKGROUND: The serine/threonine mammalian Ste-20 like kinases (MSTs are key regulators of apoptosis, cellular proliferation as well as polarization. Deregulation of MSTs has been associated with disease progression in prostate and colorectal cancer. The four human MSTs are regulated differently by C-terminal regions flanking the catalytic domains. PRINCIPAL FINDINGS: We have determined the crystal structure of kinase domain of MST4 in complex with an ATP-mimetic inhibitor. This is the first structure of an inactive conformation of a member of the MST kinase family. Comparison with active structures of MST3 and MST1 revealed a dimeric association of MST4 suggesting an activation loop exchanged mechanism of MST4 auto-activation. Together with a homology model of MST2 we provide a comparative analysis of the kinase domains for all four members of the human MST family. SIGNIFICANCE: The comparative analysis identified new structural features in the MST ATP binding pocket and has also defined the mechanism for autophosphorylation. Both structural features may be further explored for inhibitors design. ENHANCED VERSION: This article can also be viewed as an enhanced version in which the text of the article is integrated with interactive 3D representations and animated transitions. Please note that a web plugin is required to access this enhanced functionality. Instructions for the installation and use of the web plugin are available in Text S1.

  20. Cognitive diversity and moral enhancement.

    Science.gov (United States)

    Gyngell, Chris; Easteal, Simon

    2015-01-01

    One debate in contemporary bioethics centers on whether the development of cognitive enhancement technologies (CETs) will hasten the need for moral enhancement. In this article we provide a new argument in favor of pursuing these enhancement technologies together. The widespread availability of CETs will likely increase population-level cognitive diversity. Different people will choose to enhance different aspects of their cognition, and some won't enhance themselves at all. Although this has the potential to be beneficial for society, it could also result in harms as people become more different from one another. Aspects of our moral psychology make it difficult for people to cooperate and coordinate actions with those who are very different from themselves. These moral failings could be targeted by moral enhancement technologies, which may improve cooperation among individuals. Moral enhancement technologies will therefore help society maximize the benefits, and reduce the costs, associated with widespread access to cognitive enhancements.

  1. Using Microbial Source Tracking to Enhance Environmental Stewardship of Agriculture

    Science.gov (United States)

    Martin, Sherry; Rose, Joan; Flood, Matthew; Aw, Tiong; Hyndman, David

    2016-04-01

    Large scale agriculture relies on the application of chemical fertilizers and animal manure. It is well known that nutrients in excess of a plant's uptake and soil retention capacity can travel to nearby waterways via surface run-off and groundwater pathways, indirectly fertilizing these aquatic ecosystems. It has not yet been possible to distinguish water quality impacts of fertilizer from those derived from human and animal waste sources. However, new microbial source tracking (MST) tools allow specific identification of fecal pollution. Our objective was to examine pollution risks at the regional scale using MST, mapping and classification and regression tree analysis. We present results Bovine M2 genetic marker data from three flow regimes (baseflow, snow melt, and post-planting rain). Key landscape characteristics were related to the presence of the bovine markers and appear to be related to fate and transport. Impacts at this regional watershed scale will be discussed. Our research aims to identify the impacts of agricultural management practices on water quality by linking nutrient concentrations with fecal pollution sources. We hope that our research will provide guidance that will help improve water quality through agricultural best management practices to reduce pathogen contamination.

  2. Cell Growth Enhancement

    Science.gov (United States)

    1992-01-01

    Exogene Corporation uses advanced technologies to enhance production of bio-processed substances like proteins, antibiotics and amino acids. Among them are genetic modification and a genetic switch. They originated in research for Jet Propulsion Laboratory. Extensive experiments in cell growth through production of hemoglobin to improve oxygen supply to cells were performed. By improving efficiency of oxygen use by cells, major operational expenses can be reduced. Greater product yields result in decreased raw material costs and more efficient use of equipment. A broad range of applications is cited.

  3. Optical waveguide enhanced photovoltaics.

    Science.gov (United States)

    Rühle, Sven; Greenwald, Shlomit; Koren, Elad; Zaban, Arie

    2008-12-22

    Enhanced light to electric power conversion efficiency of photovoltaic cells with a low absorbance was achieved using waveguide integration. We present a proof of concept using a very thin dye-sensitized solar cell which absorbed only a small fraction of the light at normal incidence. The glass substrate in conjunction with the solar cells reflecting back contact formed a planar waveguide, which lead to more than four times higher conversion efficiency compared to conventional illumination at normal incidence. This illumination concept leads to a new type of multi-junction PV systems based on enforced spectral splitting along the waveguide.

  4. Enhancing LAN performance

    CERN Document Server

    Held, Gilbert

    2004-01-01

    Enhancing LAN Performance, Fourth Edition explains how to connect geographically separated LANs with appropriate bandwidth, the issues to consider when weighing the use of multiport or dualport devices, how to estimate traffic for new networks, the effects of configuration changes on the performance of Ethernet and Token Ring networks, the design of switch-based networks that prevent traffic bottlenecks, and other critical topics. It provides the tools to address these issues in relation to specific network requirements. This volume develops mathematical models of various LAN performance issue

  5. Universal threshold enhancement

    CERN Document Server

    Patkós, András; Szépfalusy, P; Szep, Zs.

    2003-01-01

    By assuming certain analytic properties of the propagator, it is shown that universal features of the spectral function including threshold enhancement arise if a pole describing a particle at high temperature approaches in the complex energy plane the threshold position of its two-body decay with the variation of T. The case is considered, when one can disregard any other decay processes. The quality of the proposed description is demonstrated by comparing it with the detailed large N solution of the linear sigma model around the pole-threshold coincidence.

  6. ICT Enhanced Buildings Potentials

    DEFF Research Database (Denmark)

    Christiansson, Per

    2007-01-01

    with focus on virtual building models support, new services and user environment definitions and development, virtual spaces and augmented reality, intelligent building components, application ontologies, and ICT systems integration to illustrate ICT enhanced buildings potentials and R&D needs.  ...... component systems that are accessed and integrated in the real world of building use in different contexts. The ICT systems may be physically or virtually embedded in the building. Already in 1982 AT&T established the 'intelligent buildings', IB, concept due to marketing reasons and the Informart building...

  7. Enhancing an R-matrix

    CERN Document Server

    MacKaay, M A

    1996-01-01

    In order to construct a representation of the tangle category one needs an enhanced R-matrix. In this paper we define a sufficient and necessary condition for enhancement that can be checked easily for any R-matrix. If the R-matrix can be enhanced, we also show how to construct the additional data that define the enhancement. As a direct consequence we find a sufficient condition for the construction of a knot invariant.

  8. Natural Enhancement of Color Image

    Directory of Open Access Journals (Sweden)

    Chen Shaohua

    2010-01-01

    Full Text Available A new algorithm of Natural Enhancement of Color Image (NECI is proposed. It is inspired by multiscale Retinex model. There are four steps to realize this enhancement. At first, the image appearance is rendered by content-dependent global mapping for light cast correction, and then a modified Retinex filter is applied to enhance the local contrast. Histogram rescaling is used afterwards for normalization purpose. At last, the texture details of image are enhanced by emphasizing the high-frequency components of image using multichannel decomposition of Cortex Transform. In the contrast enhancement step, luminance channel is firstly enhanced, and then a weighing map is calculated by collecting luminance enhancement information and applied to chrominance channel in color space CIELCh which enables a proportional enhancement of chrominance. It avoids the problem of unbalanced enhancement in classical RGB independent channel operation. In this work, it is believed that image enhancement should avoid dramatic modifications to image such as light condition changes, color temperature alteration, or additional artifacts introduced or amplified. Disregarding light conditions of the scene usually leads to unnaturally sharpened images or dramatic white balance changes. In the proposed method, the ambience of image (warm or cold color impression is maintained after enhancement, and no additional light sources are added to the scene, and no halo effect and blocking effect are amplified due to overenhancement. It realizes a Natural Enhancement of Color Image. Different types of natural scene images have been tested and an encouraging performance is obtained for the proposed method.

  9. Acoustically enhanced heat transport

    Energy Technology Data Exchange (ETDEWEB)

    Ang, Kar M.; Hung, Yew Mun; Tan, Ming K., E-mail: tan.ming.kwang@monash.edu [School of Engineering, Monash University Malaysia, 47500 Bandar Sunway, Selangor (Malaysia); Yeo, Leslie Y. [Micro/Nanophysics Research Laboratory, RMIT University, Melbourne, VIC 3001 (Australia); Friend, James R. [Department of Mechanical and Aerospace Engineering, University of California, San Diego, California 92093 (United States)

    2016-01-15

    We investigate the enhancement of heat transfer in the nucleate boiling regime by inducing high frequency acoustic waves (f ∼ 10{sup 6} Hz) on the heated surface. In the experiments, liquid droplets (deionized water) are dispensed directly onto a heated, vibrating substrate. At lower vibration amplitudes (ξ{sub s} ∼ 10{sup −9} m), the improved heat transfer is mainly due to the detachment of vapor bubbles from the heated surface and the induced thermal mixing. Upon increasing the vibration amplitude (ξ{sub s} ∼ 10{sup −8} m), the heat transfer becomes more substantial due to the rapid bursting of vapor bubbles happening at the liquid-air interface as a consequence of capillary waves travelling in the thin liquid film between the vapor bubble and the air. Further increases then lead to rapid atomization that continues to enhance the heat transfer. An acoustic wave displacement amplitude on the order of 10{sup −8} m with 10{sup 6} Hz order frequencies is observed to produce an improvement of up to 50% reduction in the surface temperature over the case without acoustic excitation.

  10. Acoustically enhanced heat transport

    Science.gov (United States)

    Ang, Kar M.; Yeo, Leslie Y.; Friend, James R.; Hung, Yew Mun; Tan, Ming K.

    2016-01-01

    We investigate the enhancement of heat transfer in the nucleate boiling regime by inducing high frequency acoustic waves (f ˜ 106 Hz) on the heated surface. In the experiments, liquid droplets (deionized water) are dispensed directly onto a heated, vibrating substrate. At lower vibration amplitudes (ξs ˜ 10-9 m), the improved heat transfer is mainly due to the detachment of vapor bubbles from the heated surface and the induced thermal mixing. Upon increasing the vibration amplitude (ξs ˜ 10-8 m), the heat transfer becomes more substantial due to the rapid bursting of vapor bubbles happening at the liquid-air interface as a consequence of capillary waves travelling in the thin liquid film between the vapor bubble and the air. Further increases then lead to rapid atomization that continues to enhance the heat transfer. An acoustic wave displacement amplitude on the order of 10-8 m with 106 Hz order frequencies is observed to produce an improvement of up to 50% reduction in the surface temperature over the case without acoustic excitation.

  11. Telemetry-Enhancing Scripts

    Science.gov (United States)

    Maimone, Mark W.

    2009-01-01

    Scripts Providing a Cool Kit of Telemetry Enhancing Tools (SPACKLE) is a set of software tools that fill gaps in capabilities of other software used in processing downlinked data in the Mars Exploration Rovers (MER) flight and test-bed operations. SPACKLE tools have helped to accelerate the automatic processing and interpretation of MER mission data, enabling non-experts to understand and/or use MER query and data product command simulation software tools more effectively. SPACKLE has greatly accelerated some operations and provides new capabilities. The tools of SPACKLE are written, variously, in Perl or the C or C++ language. They perform a variety of search and shortcut functions that include the following: Generating text-only, Event Report-annotated, and Web-enhanced views of command sequences; Labeling integer enumerations with their symbolic meanings in text messages and engineering channels; Systematic detecting of corruption within data products; Generating text-only displays of data-product catalogs including downlink status; Validating and labeling of commands related to data products; Performing of convenient searches of detailed engineering data spanning multiple Martian solar days; Generating tables of initial conditions pertaining to engineering, health, and accountability data; Simplified construction and simulation of command sequences; and Fast time format conversions and sorting.

  12. Direct vs. Indirect Moral Enhancement.

    Science.gov (United States)

    Schaefer, G Owen

    2015-09-01

    Moral enhancement is an ostensibly laudable project. Who wouldn't want people to become more moral? Still, the project's approach is crucial. We can distinguish between two approaches for moral enhancement: direct and indirect. Direct moral enhancements aim at bringing about particular ideas, motives or behaviors. Indirect moral enhancements, by contrast, aim at making people more reliably produce the morally correct ideas, motives or behaviors without committing to the content of those ideas, motives and/or actions. I will argue, on Millian grounds, that the value of disagreement puts serious pressure on proposals for relatively widespread direct moral enhancement. A more acceptable path would be to focus instead on indirect moral enhancements while staying neutral, for the most part, on a wide range of substantive moral claims. I will outline what such indirect moral enhancement might look like, and why we should expect it to lead to general moral improvement.

  13. Enhanced Geothermal Systems

    Energy Technology Data Exchange (ETDEWEB)

    Jeanloz, R. [The MITRE Corporation, McLean, VA (United States); Stone, H. [The MITRE Corporation, McLean, VA (United States); et al.

    2013-12-31

    DOE, through the Geothermal Technologies Office (GTO) within the Office of Energy Efficiency and Renewable Energy, requested this study, identifying a focus on: i) assessment of technologies and approaches for subsurface imaging and characterization so as to be able to validate EGS opportunities, and ii) assessment of approaches toward creating sites for EGS, including science and engineering to enhance permeability and increase the recovery factor. Two days of briefings provided in-depth discussion of a wide range of themes and challenges in EGS, and represented perspectives from industry, government laboratories and university researchers. JASON also contacted colleagues from universities, government labs and industry in further conversations to learn the state of the field and potential technologies relevant to EGS.

  14. Surface enhanced Raman scattering

    CERN Document Server

    Furtak, Thomas

    1982-01-01

    In the course of the development of surface science, advances have been identified with the introduction of new diagnostic probes for analytical characterization of the adsorbates and microscopic structure of surfaces and interfaces. Among the most recently de­ veloped techniques, and one around which a storm of controversy has developed, is what has now been earmarked as surface enhanced Raman scattering (SERS). Within this phenomenon, molecules adsorbed onto metal surfaces under certain conditions exhibit an anomalously large interaction cross section for the Raman effect. This makes it possible to observe the detailed vibrational signature of the adsorbate in the ambient phase with an energy resolution much higher than that which is presently available in electron energy loss spectroscopy and when the surface is in contact with a much larger amount of material than that which can be tolerated in infrared absorption experiments. The ability to perform vibrational spectroscopy under these conditions would l...

  15. Structure Size Enhanced Histogram

    Science.gov (United States)

    Wesarg, Stefan; Kirschner, Matthias

    Direct volume visualization requires the definition of transfer functions (TFs) for the assignment of opacity and color. Multi-dimensional TFs are based on at least two image properties, and are specified by means of 2D histograms. In this work we propose a new type of a 2D histogram which combines gray value with information about the size of the structures. This structure size enhanced (SSE) histogram is an intuitive approach for representing anatomical features. Clinicians — the users we are focusing on — are much more familiar with selecting features by their size than by their gradient magnitude value. As a proof of concept, we employ the SSE histogram for the definition of two-dimensional TFs for the visualization of 3D MRI and CT image data.

  16. Cavity enhanced atomic magnetometry

    CERN Document Server

    Crepaz, Herbert; Dumke, Rainer

    2015-01-01

    Atom sensing based on Faraday rotation is an indispensable method for precision measurements, universally suitable for both hot and cold atomic systems. Here we demonstrate an all-optical magnetometer where the optical cell for Faraday rotation spectroscopy is augmented with a low finesse cavity. Unlike in previous experiments, where specifically designed multipass cells had been employed, our scheme allows to use conventional, spherical vapour cells. Spherical shaped cells have the advantage that they can be effectively coated inside with a spin relaxation suppressing layer providing long spin coherence times without addition of a buffer gas. Cavity enhancement shows in an increase in optical polarization rotation and sensitivity compared to single-pass configurations.

  17. Enhancing the network synchronizability

    Institute of Scientific and Technical Information of China (English)

    ZHAO Ming; ZHOU Tao; CHEN Guan-rong; WANG Bing-hong

    2007-01-01

    The structural and dynamical properties, partic-ularly the small-world effect and scale-free feature, of com-plex networks have attracted tremendous interest and atten-tion in recent years. This article offers a brief review of one focal issue concerning the structural and dynamical behav-iors of complex network synchronization. In the presenta-tion, the notions of synchronization of dynamical systems on networks, stability of dynamical networks, and relationships between network structure and synchronizability, will be first introduced. Then, various technical methods for enhancing the network synchronizability will be discussed, which are roughly divided into two classes: Structural Modification and Coupling-Pattern Regulation, where the former includes three typical methods-dividing hub nodes, shortening average dis-tances, and deleting overload edges, while the latter mainly is a method of strengthening the hub-nodes' influence on the network.

  18. Teacher Enhancement Institute

    Science.gov (United States)

    Marshall-Bradley, Tina

    1994-01-01

    During the 1980's, a period of intense concern over educational quality in the United States, few indicators of U.S. student achievement garnered the interest of policy makers and pundits as successfully as the results of international testing in mathematics and science. This concern was so great that as a part of the Goals 2000 initiative, President George Bush indicated that 'By the year 2000, U.S. students should be first in the world in mathematics and science.' The Clinton Administration is placing a major emphasis, not only on rigorous academic standards and creating a new system for assessing students' progress, but also including professional development as a major focus. The argument being that teachers need more sustained, intensive training to prepare them to teach to higher standards. Executive order 12821 mandates that national laboratories 'assist in the mathematics and science education of our Nation's students, teachers, parents and the public by establishing programs at their agency to provide for training elementary and secondary school teachers to improve their knowledge of mathematics and science'. These and other issues led to the development of ideas for a project that addresses the need for excellence in mathematics, science and technology instruction. In response to these initiatives the NASA/LaRC Teacher Enhancement Institute was proposed. The TEI incorporated systemic reform perspectives, enhanced content knowledge for teachers, and teacher preparation. Emphasis was also placed on recruiting those educators who teach in impoverished urban school districts with at-risk student populations who have been traditionally under represented in science, mathematics, technology and engineering. Participants in the Teacher Enhancement Institute were 37 teachers from grades K-8, teaching in Region 2 in the state of Virginia, as well as 2 preservice teachers from Norfolk State University and one teacher from Dublin, Virginia, where a Science

  19. Surface enhanced thermo lithography

    KAUST Repository

    Coluccio, Maria Laura

    2017-01-13

    We used electroless deposition to fabricate clusters of silver nanoparticles (NPs) on a silicon substrate. These clusters are plasmonics devices that induce giant electromagnetic (EM) field increments. When those EM field are absorbed by the metal NPs clusters generate, in turn, severe temperature increases. Here, we used the laser radiation of a conventional Raman set-up to transfer geometrical patterns from a template of metal NPs clusters into a layer of thermo sensitive Polyphthalaldehyde (PPA) polymer. Temperature profile on the devices depends on specific arrangements of silver nanoparticles. In plane temperature variations may be controlled with (i) high nano-meter spatial precision and (ii) single Kelvin temperature resolution on varying the shape, size and spacing of metal nanostructures. This scheme can be used to generate strongly localized heat amplifications for applications in nanotechnology, surface enhanced thermo-lithography (SETL), biology and medicine (for space resolved cell ablation and treatment), nano-chemistry.

  20. Dimensional Enhancement via Supersymmetry

    CERN Document Server

    Faux, M G; Landweber, G D

    2009-01-01

    We explain how the representation theory associated with supersymmetry in diverse dimensions is encoded within the representation theory of supersymmetry in one time-like dimension. This is enabled by algebraic criteria, derived, exhibited, and utilized in this paper, which indicate which subset of one-dimensional supersymmetric models describe "shadows" of higher-dimensional models. This formalism delineates that minority of one-dimensional supersymmetric models which can "enhance" to accommodate extra dimensions. As a consistency test, we use our formalism to reproduce well-known conclusions about supersymmetric field theories using one-dimensional reasoning exclusively. And we introduce the notion of "phantoms" which usefully accommodate higher-dimensional gauge invariance in the context of shadow multiplets in supersymmetric quantum mechanics.

  1. Cavity enhanced atomic magnetometry.

    Science.gov (United States)

    Crepaz, Herbert; Ley, Li Yuan; Dumke, Rainer

    2015-10-20

    Atom sensing based on Faraday rotation is an indispensable method for precision measurements, universally suitable for both hot and cold atomic systems. Here we demonstrate an all-optical magnetometer where the optical cell for Faraday rotation spectroscopy is augmented with a low finesse cavity. Unlike in previous experiments, where specifically designed multipass cells had been employed, our scheme allows to use conventional, spherical vapour cells. Spherical shaped cells have the advantage that they can be effectively coated inside with a spin relaxation suppressing layer providing long spin coherence times without addition of a buffer gas. Cavity enhancement shows in an increase in optical polarization rotation and sensitivity compared to single-pass configurations.

  2. Micro–Positron Emission Tomography/Contrast-Enhanced Computed Tomography Imaging of Orthotopic Pancreatic Tumor–Bearing Mice Using the αvβ3 Integrin Tracer 64Cu-Labeled Cyclam-RAFT-c(-RGDfK-4

    Directory of Open Access Journals (Sweden)

    Winn Aung

    2013-09-01

    Full Text Available The purpose of this study was to develop a clinically relevant orthotopic xenotransplantation model of pancreatic cancer and to perform a preclinical evaluation of a new positron emission tomography (PET imaging probe, 64Cu-labeled cyclam-RAFT-c(-RGDfK-4 peptide (64Cu-RAFT-RGD, using this model. Varying degrees of αvβ3 integrin expression in several human pancreatic cancer cell lines were examined by flow cytometry and Western blotting. The cell line BxPC-3, which is stably transfected with a red fluorescence protein (RFP, was used for surgical orthotopic implantation. Orthotopic xenograft was established in the pancreas of recipient nude mice. An in vivo probe biodistribution and receptor blocking study, preclinical PET imaging coregistered with contrast-enhanced computed tomography (CECT comparing 64Cu-RAFT-RGD and 18F-fluoro-2-deoxy-D-glucose (18F-FDG accumulation in tumor, postimaging autoradiography, and histologic and immunohistochemical examinations were done. Biodistribution evaluation with a blocking study confirmed that efficient binding of probe to tumor is highly αvβ3 integrin specific. 64Cu-RAFT-RGD PET combined with CECT provided for precise and easy detection of cancer lesions. Autoradiography, histologic, and immunohistochemical examinations confirmed the accumulation of 64Cu-RAFT-RGD in tumor versus nontumor tissues. In comparative PET studies, 64Cu-RAFT-RGD accumulation provided better tumor contrast to background than 18F-FDG. Our results suggest that 64Cu-RAFT-RGD PET imaging is potentially applicable for the diagnosis of αvβ3 integrin–expressing pancreatic tumors.

  3. Fokker-Planck Simulation of Fast Wave Current Drive and Heating in the Reversed Field Pinch

    Science.gov (United States)

    Uchimoto, E.; Shiina, S.; Harvey, R. W.; Smirnov, A. P.; Forest, C. B.; Prager, S. C.; Wright, J. C.

    1999-11-01

    Fast wave current drive (FWCD) has been shown theoretically to be a good candidate for improving plasma confinement characteristics of a high-beta, reactor-grade RFP via current profile control.footnote S. Shiina, Y. Kondoh, H. Ishii, Nuclear Fusion 34, 1473 (1994); T. Nagai et al., Proc. ICPP (Nagoya, 1996), p. 1042; K. Kusano et al., 17th IAEA Fusion Energy Conf. (Yokohama, 1998), paper THP1/12. To assess the effects of toroidicity and quasilinear modifications to the electron distribution function on FWCD, we are using the RFP version of ray-tracing and Fokker-Planck codes (GENRAY and CQL3D). Although lower hybrid slow waves are ideally suited for poloidal current drive in large RFPs presently in operation, possible use of fast waves is being considered for core current drive and heating in these devices. For MST parameters, our calculations focus on intermediate to high harmonic fast waves for which geometric optics is valid.

  4. Lignite Fuel Enhancement

    Energy Technology Data Exchange (ETDEWEB)

    Charles Bullinger; Nenad Sarunac

    2010-03-31

    Pulverized coal power plants which fire lignites and other low-rank high-moisture coals generally operate with reduced efficiencies and increased stack emissions due to the impacts of high fuel moisture on stack heat loss and pulverizer and fan power. A process that uses plant waste heat sources to evaporate a portion of the fuel moisture from the lignite feedstock in a moving bed fluidized bed dryer (FBD) was developed in the U.S. by a team led by Great River Energy (GRE). The demonstration was conducted with Department of Energy (DOE) funding under DOE Award Number DE-FC26-04NT41763. The objectives of GRE's Lignite Fuel Enhancement project were to demonstrate reduction in lignite moisture content by using heat rejected from the power plant, apply technology at full scale at Coal Creek Station (CCS), and commercialize it. The Coal Creek Project has involved several stages, beginning with lignite drying tests in a laboratory-scale FBD at the Energy Research Center (ERC) and development of theoretical models for predicting dryer performance. Using results from these early stage research efforts, GRE built a 2 ton/hour pilot-scale dryer, and a 75 ton/hour prototype drying system at Coal Creek Station. Operated over a range of drying conditions, the results from the pilot-scale and prototype-scale dryers confirmed the performance of the basic dryer design concept and provided the knowledge base needed to scale the process up to commercial size. Phase 2 of the GRE's Lignite Fuel Enhancement project included design, construction and integration of a full-scale commercial coal drying system (four FBDs per unit) with Coal Creek Units 1 and 2 heat sources and coal handling system. Two series of controlled tests were conducted at Coal Creek Unit 1 with wet and dried lignite to determine effect of dried lignite on unit performance and emissions. Wet lignite was fired during the first, wet baseline, test series conducted in September 2009. The second test series was

  5. Color image and video enhancement

    CERN Document Server

    Lecca, Michela; Smolka, Bogdan

    2015-01-01

    This text covers state-of-the-art color image and video enhancement techniques. The book examines the multivariate nature of color image/video data as it pertains to contrast enhancement, color correction (equalization, harmonization, normalization, balancing, constancy, etc.), noise removal and smoothing. This book also discusses color and contrast enhancement in vision sensors and applications of image and video enhancement.   ·         Focuses on enhancement of color images/video ·         Addresses algorithms for enhancing color images and video ·         Presents coverage on super resolution, restoration, in painting, and colorization.

  6. Backside configured surface plasmonic enhancement

    Energy Technology Data Exchange (ETDEWEB)

    Gu, Guiru; Lu, Xuejun, E-mail: xuejun-lu@uml.edu [Department of Electrical and Computer Engineering, University of Massachusetts Lowell, One University Avenue, Lowell, MA 01854 (United States); Vaillancourt, Jarrod [Applied NanoFemto Technologies, LLC, 181 Stedman St. 2, Lowell, MA 01851 (United States)

    2014-03-31

    In this work, we fabricated, measured and compared the quantum dots infrared photodetector enhancement by the top- and backside- configured plasmonic structures. The backside configured plasmonic structure can provide much higher device performance enhancement. Furthermore, the excitation of the surface plasmonic waves by the top- and backside- configured plasmonic structures was analyzed. Detailed simulation results of the electric field at different wavelength from top illumination and backside illumination were provided. The stronger electric field from the backside illumination attributed to the higher enhancement.

  7. Development of enhanced radioprotectors

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Jin Kyu; Chun, K. J.; Lee, Y. K.; Kim, Y. K.; Lee, B. H.; Song, H. S.; Lee, C. J.; Chung, K. M.; Shin, H. S.; Park, T. W

    2000-04-01

    Biological model systems such as TSH mutations, animals and their reproductive organs, and cultured cell lines and human peripheral lymphocytes have been set up for screening radioprotective or radiosensitizing activities of natural products. About 100 natural plants were in vivo and in vitro tested. Among them, Salvia miltiorrhiza, Areca catechu, Cornus officinalis, and portulaca oleracea showed 20 percent of radioprotective activities. On the other hand, Acanthopanax sessiliflous, Melia japonica, and Agastache rugosa revealed radiosensitizing activities. Experimental studies on the farnesyl transferase inhibitors showed the results that some of the inhibitors lead to higher levels of cell death after {gamma}-irradiation and increased radiosensitivity in H-ras-transformed NIH3T3 cells and MCF-10A human tumor cells. Molecular biochemical studies on the mechanism of radioprotection was carried out using cultured cells. The treatment of DTT increased cell survival after gamma-irradiation,decreased in the frequencies of micronucleus, and reduction in DNA fragmentation and apoptotic cells. Induction of apoptosis after irradiation was revealed by the changes in the relative cell death, increase in the relative amount of apoptotic cells, and the induction of DNA fragmentation. The result of mechanism studies will be applied to elucidate the radioprotection mechanism and to develop enhanced radioprotectors. Furthermore, our results indicate that use of antioxidants in combination can efficiently modulate the radiation-induced cell damage like apoptosis and can be applied to alleviate the cellular damage in tumor patients during radiotherapy. (author)

  8. Enhanced Design Alternative IV

    Energy Technology Data Exchange (ETDEWEB)

    N. E. Kramer

    1999-05-18

    This report evaluates Enhanced Design Alternative (EDA) IV as part of the second phase of the License Application Design Selection (LADS) effort. The EDA IV concept was compared to the VA reference design using criteria from the ''Design Input Request for LADS Phase II EDA Evaluations'' (CRWMS M&O 1999b) and (CRWMS M&O 1999f). Briefly, the EDA IV concept arranges the waste packages close together in an emplacement configuration known as ''line load''. Continuous pre-closure ventilation keeps the waste packages from exceeding the 350 C cladding and 200 C (4.3.13) drift wall temperature limits. This EDA concept keeps relatively high, uniform emplacement drift temperatures (post-closure) to drive water away from the repository and thus dry out the pillars between emplacement drifts. The waste package is shielded to permit human access to emplacement drifts and includes an integral filler inside the package to reduce the amount of water that can contact the waste form. Closure of the repository is desired 50 years after first waste is emplaced. Both backfill and a drip shields will be emplaced at closure to improve post-closure performance.

  9. Development of enhanced radioprotectors

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Jin Kyu; Park, K.B.; Lee, Y.I. [and others

    1999-04-01

    Biological model systems such as TSH mutations, animals and their reproductive organs, and cultured cell lines has been set up for screening radioprotective or radiosensitizing activities of natural products. About 100 natural plants were in vivo and in vitro tested. Among them, Salvia miltiorrhiza, Areca catechu, Cornus officinalis, and Portulaca oleracea showed 20% of radioprotective activities. On the other hand, Acanthopanax sessiliflous, Melia japonica, and Agastache rugosa revealed radiosensitizing activities. Experimental studies on the farnesyltransferase inhibitors showed the results that some of the inhibits lead to higher levels of cell death after {gamma} -irradiation and increased radiosensitivity in H-ras-transformed NIH3T3 cells and MCF-10A human tumor cells. Molecular biochemical studies on the mechanism of radioprotection was carried out using cultured cells. The treatment of DTT increased cell survival after gamma-irradiation, decreased in the frequencies of micronucleus, and reduction in DNA fragmentation and apoptotic cells. Induction of apoptosis after irradiation was revealed by the changes in the relative cell death, increase in the relative amount of apoptotic cells, and the induction of DNA fragmentation. Samples shown excellent radioprotective or radiosensitizing activities will be subjected to purification to develop radioprotectants and radiosensitizers applicable to human beings. The result of mechanism studies will be applied to elucidate the radioprotection mechanism and the develop enhanced radioprotectors. (author). 145 refs., 21 tabs., 29 figs.

  10. Enhanced Micellar Catalysis LDRD.

    Energy Technology Data Exchange (ETDEWEB)

    Betty, Rita G.; Tucker, Mark D; Taggart, Gretchen; Kinnan, Mark K.; Glen, Crystal Chanea; Rivera, Danielle; Sanchez, Andres; Alam, Todd Michael

    2012-12-01

    The primary goals of the Enhanced Micellar Catalysis project were to gain an understanding of the micellar environment of DF-200, or similar liquid CBW surfactant-based decontaminants, as well as characterize the aerosolized DF-200 droplet distribution and droplet chemistry under baseline ITW rotary atomization conditions. Micellar characterization of limited surfactant solutions was performed externally through the collection and measurement of Small Angle X-Ray Scattering (SAXS) images and Cryo-Transmission Electron Microscopy (cryo-TEM) images. Micellar characterization was performed externally at the University of Minnesotas Characterization Facility Center, and at the Argonne National Laboratory Advanced Photon Source facility. A micellar diffusion study was conducted internally at Sandia to measure diffusion constants of surfactants over a concentration range, to estimate the effective micelle diameter, to determine the impact of individual components to the micellar environment in solution, and the impact of combined components to surfactant phase behavior. Aerosolized DF-200 sprays were characterized for particle size and distribution and limited chemical composition. Evaporation rates of aerosolized DF-200 sprays were estimated under a set of baseline ITW nozzle test system parameters.

  11. Enhancing a Universe Below

    Energy Technology Data Exchange (ETDEWEB)

    Berge, Gunnar

    1998-12-31

    By understanding the past and by means of modern technology the petroleum resources can be exploited in an environmentally friendly and cost-effective way. Since the future of the oil and gas industry depends on the size and availability of the remaining resources and on the demand for the products, this presentation aims to overview these resources, globally and on the Norwegian Shelf. It also considers the uncertainty in the future demand for oil and gas. A key challenge for the industry is to enhance the value of these resources without compromising environmental values. The industry will also have to face issues such as deregulation of the energy markets, hydrocarbon taxation, etc. These challenges also provide possibilities for a proactive industry; how can we make the industry more competitive? New oil and gas fields are now being developed in deeper water, in harsh climate conditions and in vulnerable environments. These challenges call for technological development and strong focus on safety and environmental issues. Long-term commercial commitment and cooperation of the industry is essential for new technologies and for training. 2 figs.

  12. Multispectral histogram normalization contrast enhancement

    Science.gov (United States)

    Soha, J. M.; Schwartz, A. A.

    1979-01-01

    A multispectral histogram normalization or decorrelation enhancement which achieves effective color composites by removing interband correlation is described. The enhancement procedure employs either linear or nonlinear transformations to equalize principal component variances. An additional rotation to any set of orthogonal coordinates is thus possible, while full histogram utilization is maintained by avoiding the reintroduction of correlation. For the three-dimensional case, the enhancement procedure may be implemented with a lookup table. An application of the enhancement to Landsat multispectral scanning imagery is presented.

  13. What are super-enhancers?

    Science.gov (United States)

    Pott, Sebastian; Lieb, Jason D

    2015-01-01

    The term 'super-enhancer' has been used to describe groups of putative enhancers in close genomic proximity with unusually high levels of Mediator binding, as measured by chromatin immunoprecipitation and sequencing (ChIP-seq). Here we review the identification and composition of super-enhancers, describe links between super-enhancers, gene regulation and disease, and discuss the functional significance of enhancer clustering. We also provide our perspective regarding the proposition that super-enhancers are a regulatory entity conceptually distinct from what was known before the introduction of the term. Our opinion is that there is not yet strong evidence that super-enhancers are a novel paradigm in gene regulation and that use of the term in this context is not currently justified. However, the term likely identifies strong enhancers that exhibit behaviors consistent with previous models and concepts of transcriptional regulation. In this respect, the super-enhancer definition is useful in identifying regulatory elements likely to control genes important for cell type specification.

  14. PENETRATION ENHANCERS: A NOVEL STRATEGY FOR ENHANCING TRANSDERMAL DRUG DELIVERY

    Directory of Open Access Journals (Sweden)

    Singla Vikas

    2011-12-01

    Full Text Available Skin penetration enhancers have been used to improve bioavailability and increase the range of drugs to be administered by topical and transdermal route. Enhancement in skin penetration via modification of the stratum corneum by hydration, or via use of chemical enhancers acting on the structure of the stratum corneum lipids and keratin, partitioning and solubility effects. The mechanism of action of penetration enhancers are used as an aid in potential clinical applications. Synthetic chemicals generally used for this purposes are rapidly losing their value in transdermal patches due to reports of their absorption into the systemic circulation and subsequent possible toxic effect upon long term application. Terpenes are included in the list of Generally Recognized as Safe (GRAS substances and have low irritancy potential. In this review, we have discussed the chemical penetration as well as natural penetration enhancement technology for transdermal drug delivery as well as the probable mechanisms of action.

  15. Enhancers, enhancers - from their discovery to today's universe of transcription enhancers.

    Science.gov (United States)

    Schaffner, Walter

    2015-04-01

    Transcriptional enhancers are short (200-1500 base pairs) DNA segments that are able to dramatically boost transcription from the promoter of a target gene. Originally discovered in simian virus 40 (SV40), a small DNA virus, transcription enhancers were soon also found in immunoglobulin genes and other cellular genes as key determinants of cell-type-specific gene expression. Enhancers can exert their effect over long distances of thousands, even hundreds of thousands of base pairs, either from upstream, downstream, or from within a transcription unit. The number of enhancers in eukaryotic genomes correlates with the complexity of the organism; a typical mammalian gene is likely controlled by several enhancers to fine-tune its expression at different developmental stages, in different cell types and in response to different signaling cues. Here, I provide a personal account of how enhancers were discovered more than 30 years ago, and also address the amazing development of the field since then.

  16. Plasmon Enhanced Photoemission

    Energy Technology Data Exchange (ETDEWEB)

    Polyakov, Aleksandr [Univ. of California, Berkeley, CA (United States)

    2012-05-08

    this work, the structure consisted of rectangular nano-grooves (NGs) arranged in a subwavelength grating on a metal surface is presented that provides a dramatic increase in the metal’s absorption, field localization, and field enhancement. When light is polarized perpendicular to the orientation of the grooves a standing SPP wave is excited along the vertical walls in the NGs, that act as Fabry-Perot resonators. By adjusting the geometry of the NGs and the period of the subwavelength grating the resonance can be fine tuned to a desired position, for example, the laser fundamental wavelength, anywhere from the UV to the near infrared (NIR). Two types of gratings are presented: (a) a gold grating with period of 600 nm, and (b) an aluminum-gold grating with a period of 100 nm; both with resonance at 720 nm. In each case, strong on-resonance absorption was observed, with over 98% for grating (b). Unlike the grating-coupled SPP waves, where the angle is well defined by the momentum matching condition, the resonant NGs allow coupling to the standing modes at a range of angles of incidence, referred to as the angular bandwidth. A new model for the on-resonance absorption based on the ensamble action of the NGs is presented that serves as the basis for a design of an NG grating with an ultrawide spectral as well as angular bandwidth. For sample (b), the angular bandwidth is 80 degrees, corresponding to an opening angle of 160 degrees. The photoemission enhancement for such a grating was measured to be seven orders of magnitude for a four-photon photoemission. This is an incredible result demonstrating the power of the plasmonic grating presented, which is an efficient light trapper and field enhancer for a non-linear processes. These results demonstrate that the metal photocathode prepared with a NG grating on the metal surface will provide sufficient pulse charge driven by a 1 μJ 15fs pulsed laser at 800 nm for the optimum FEL operation.

  17. Motif signatures of transcribed enhancers

    KAUST Repository

    Kleftogiannis, Dimitrios

    2017-09-14

    In mammalian cells, transcribed enhancers (TrEn) play important roles in the initiation of gene expression and maintenance of gene expression levels in spatiotemporal manner. One of the most challenging questions in biology today is how the genomic characteristics of enhancers relate to enhancer activities. This is particularly critical, as several recent studies have linked enhancer sequence motifs to specific functional roles. To date, only a limited number of enhancer sequence characteristics have been investigated, leaving space for exploring the enhancers genomic code in a more systematic way. To address this problem, we developed a novel computational method, TELS, aimed at identifying predictive cell type/tissue specific motif signatures. We used TELS to compile a comprehensive catalog of motif signatures for all known TrEn identified by the FANTOM5 consortium across 112 human primary cells and tissues. Our results confirm that distinct cell type/tissue specific motif signatures characterize TrEn. These signatures allow discriminating successfully a) TrEn from random controls, proxy of non-enhancer activity, and b) cell type/tissue specific TrEn from enhancers expressed and transcribed in different cell types/tissues. TELS codes and datasets are publicly available at http://www.cbrc.kaust.edu.sa/TELS.

  18. Metaheuristic applications to speech enhancement

    CERN Document Server

    Kunche, Prajna

    2016-01-01

    This book serves as a basic reference for those interested in the application of metaheuristics to speech enhancement. The major goal of the book is to explain the basic concepts of optimization methods and their use in heuristic optimization in speech enhancement to scientists, practicing engineers, and academic researchers in speech processing. The authors discuss why it has been a challenging problem for researchers to develop new enhancement algorithms that aid in the quality and intelligibility of degraded speech. They present powerful optimization methods to speech enhancement that can help to solve the noise reduction problems. Readers will be able to understand the fundamentals of speech processing as well as the optimization techniques, how the speech enhancement algorithms are implemented by utilizing optimization methods, and will be given the tools to develop new algorithms. The authors also provide a comprehensive literature survey regarding the topic.

  19. Resolution Enhancement of Multilook Imagery

    Energy Technology Data Exchange (ETDEWEB)

    Galbraith, Amy E. [Univ. of Arizona, Tucson, AZ (United States)

    2004-07-01

    This dissertation studies the feasibility of enhancing the spatial resolution of multi-look remotely-sensed imagery using an iterative resolution enhancement algorithm known as Projection Onto Convex Sets (POCS). A multi-angle satellite image modeling tool is implemented, and simulated multi-look imagery is formed to test the resolution enhancement algorithm. Experiments are done to determine the optimal con guration and number of multi-angle low-resolution images needed for a quantitative improvement in the spatial resolution of the high-resolution estimate. The important topic of aliasing is examined in the context of the POCS resolution enhancement algorithm performance. In addition, the extension of the method to multispectral sensor images is discussed and an example is shown using multispectral confocal fluorescence imaging microscope data. Finally, the remote sensing issues of atmospheric path radiance and directional reflectance variations are explored to determine their effect on the resolution enhancement performance.

  20. Enhanced incoherent scatter plasma lines

    Directory of Open Access Journals (Sweden)

    H. Nilsson

    Full Text Available Detailed model calculations of auroral secondary and photoelectron distributions for varying conditions have been used to calculate the theoretical enhancement of incoherent scatter plasma lines. These calculations are compared with EISCAT UHF radar measurements of enhanced plasma lines from both the E and F regions, and published EISCAT VHF radar measurements. The agreement between the calculated and observed plasma line enhancements is good. The enhancement from the superthermal distribution can explain even the very strong enhancements observed in the auroral E region during aurora, as previously shown by Kirkwood et al. The model calculations are used to predict the range of conditions when enhanced plasma lines will be seen with the existing high-latitude incoherent scatter radars, including the new EISCAT Svalbard radar. It is found that the detailed structure, i.e. the gradients in the suprathermal distribution, are most important for the plasma line enhancement. The level of superthermal flux affects the enhancement only in the region of low phase energy where the number of thermal electrons is comparable to the number of suprathermal electrons and in the region of high phase energy where the suprathermal fluxes fall to such low levels that their effect becomes small compared to the collision term. To facilitate the use of the predictions for the different radars, the expected signal- to-noise ratios (SNRs for typical plasma line enhancements have been calculated. It is found that the high-frequency radars (Søndre Strømfjord, EISCAT UHF should observe the highest SNR, but only for rather high plasma frequencies. The VHF radars (EISCAT VHF and Svalbard will detect enhanced plasma lines over a wider range of frequencies, but with lower SNR.

  1. Environmental engineering education enhancement

    Science.gov (United States)

    Caporali, E.

    2012-04-01

    Since higher education plays a central role in the development of both human beings and modern societies, enhancing social, cultural and economic development, active citizenship, ethical values and expertises for a sustainable growth, environment respectful, the European Commission promotes a wide range of programmes. Among the EC programmes, the TEMPUS - Trans European Mobility Programme for University Studies, with the support of the DG EAC of the European Commission, has contributed to many aspects of general interest for higher education. Curricula harmonization, LifeLong Learning Programme development, ICT use, quality assessment, accreditation, innovation learning methods, growth of networks of institutions trusting each other, are the focused aspects. Such a solid cooperation framework is surely among the main outcomes of the TEMPUS Projects leaded by the University of Firenze UNIFI (Italy), DEREC - Development of Environment and Resources Engineering Curriculum (2005-2008), and its spin-off DEREL - Development of Environment and Resources Engineering Learning (2010-2013), and VICES - Videoconferencing Educational Services (2009-2012). DEREC and DEREL TEMPUS projects, through the co-operation of Universities in Italy, Austria, Germany, Greece, Macedonia, Albania and Serbia, are aimed at the development of first and second level curricula in "Environment and Resources Engineering" at the Ss. Cyril and Methodius University - UKIM Skopje (MK). In the DEREC Project the conditions for offering a joint degree title in the field of Environmental Engineering between UNIFI and UKIM Skopje were fulfilled and a shared educational programme leading to the mutual recognition of degree titles was defined. The DEREL project, as logical continuation of DEREC, is aimed to introduce a new, up-to-date, postgraduate second level curriculum in Environment and Resources Engineering at UKIM Skopje, University of Novi Sad (RS) and Polytechnic University of Tirana (AL). following

  2. Fuzzy-Contextual Contrast Enhancement.

    Science.gov (United States)

    Parihar, Anil; Verma, Om; Khanna, Chintan

    2017-02-08

    This paper presents contrast enhancement algorithms based on fuzzy contextual information of the images. We introduce fuzzy similarity index and fuzzy contrast factor to capture the neighborhood characteristics of a pixel. A new histogram, using fuzzy contrast factor of each pixel is developed, and termed as the fuzzy dissimilarity histogram (FDH). A cumulative distribution function (CDF) is formed with normalized values of FDH and used as a transfer function to obtain the contrast enhanced image. The algorithm gives good contrast enhancement and preserves the natural characteristic of the image. In order to develop a contextual intensity transfer function, we introduce a fuzzy membership function based on fuzzy similarity index and coefficient of variation of the image. The contextual intensity transfer function is designed using the fuzzy membership function to achieve final contrast enhanced image. The overall algorithm is referred as the fuzzy contextual contrast-enhancement (FCCE) algorithm. The proposed algorithms are compared with conventional and state-of-art contrast enhancement algorithms. The quantitative and visual assessment of the results is performed. The results of quantitative measures are statistically analyzed using t-test. The exhaustive experimentation and analysis show the proposed algorithm efficiently enhances contrast and yields in natural visual quality images.

  3. Resolution enhancement in tilted coordinates

    Science.gov (United States)

    Hariri Naghadeh, Diako; Keith Morley, Christopher

    2016-11-01

    Deconvolution is applied to remove source wavelet effects from seismograms. The results are resolution enhancement that enables detection of thin layers. Following enhancement of resolution, low frequency and high angle reflectors, particularly at great depth, appear as low amplitude and semi-invisible reflectors that are difficult to track and pick. A new approach to enhance resolution is introduced that estimates a derivative using continuous wavelet transform in tilted coordinates. The results are compared with sparse spike deconvolution, curvelet deconvolution and inverse quality filtering in wavelet domain. The positive consequence of the new method is to increase sampling of high dip features by changing the coordinate system from Cartesian to tilted. To compare those methods a complex data set was chosen that includes high angle faults and chaotic mass transport complex. Image enhancement using curvelet deconvolution shows a chaotic system as a non-chaotic one. The results show that sparse spike deconvolution and inverse quality filtering in wavelet domain are able to enhance resolution more than curvelet deconvolution especially at great depth but it is impossible to follow steep dip reflectors after resolution enhancement using these methods, especially when their apparent dips are more than 45°. By estimating derivatives in a continuous wavelet transform from tilted data sets similar resolution enhancement as the other deconvolution methods is achieved but additionally steep dipping reflectors are imaged much better than others. Subtracted results of the enhanced resolution data set using new method and the other introduced methods show that steeply dipping reflectors are highlighted as a particular ability of the new method. The results show that high frequency recovery in Cartesian co-ordinate is accompanied by inability to image steeply dipping reflectors especially at great depths. Conversely recovery of high frequency data and imaging of the data

  4. Resolution enhancement techniques in microscopy

    Science.gov (United States)

    Cremer, Christoph; Masters, Barry R.

    2013-05-01

    We survey the history of resolution enhancement techniques in microscopy and their impact on current research in biomedicine. Often these techniques are labeled superresolution, or enhanced resolution microscopy, or light-optical nanoscopy. First, we introduce the development of diffraction theory in its relation to enhanced resolution; then we explore the foundations of resolution as expounded by the astronomers and the physicists and describe the conditions for which they apply. Then we elucidate Ernst Abbe's theory of optical formation in the microscope, and its experimental verification and dissemination to the world wide microscope communities. Second, we describe and compare the early techniques that can enhance the resolution of the microscope. Third, we present the historical development of various techniques that substantially enhance the optical resolution of the light microscope. These enhanced resolution techniques in their modern form constitute an active area of research with seminal applications in biology and medicine. Our historical survey of the field of resolution enhancement uncovers many examples of reinvention, rediscovery, and independent invention and development of similar proposals, concepts, techniques, and instruments. Attribution of credit is therefore confounded by the fact that for understandable reasons authors stress the achievements from their own research groups and sometimes obfuscate their contributions and the prior art of others. In some cases, attribution of credit is also made more complex by the fact that long term developments are difficult to allocate to a specific individual because of the many mutual connections often existing between sometimes fiercely competing, sometimes strongly collaborating groups. Since applications in biology and medicine have been a major driving force in the development of resolution enhancing approaches, we focus on the contribution of enhanced resolution to these fields.

  5. Dural enhancement in pituitary macroadenomas

    Energy Technology Data Exchange (ETDEWEB)

    Cattin, F. [Dept. of Neuroradiology, University Hospital of Besancon (France); Dept. of Neuroradiology, Jean Minjoz Hospital, Besancon (France); Bonneville, F.; Andrea, I.; Barrali, E.; Bonneville, J.F. [Dept. of Neuroradiology, University Hospital of Besancon (France)

    2000-07-01

    We describe the normal dural enhancement patterns of the sellar region and determine whether the duramater is affected by pituitary macroadenomas. Dural enhancement appeared to be usually abnormal in 20 patients with pituitary macroadenoma compared with 20 control patients, mainly at the planum sphenoidale and carotid sulcus. However dural changes are subtle and their recognition requires knowledge of the normal enhancement patterns. Dural changes, reported in a variety of inflammatory and infectious dural diseases and after surgery, are not specific and may be also seen in pituitary macroadenomas. (orig.)

  6. Speech enhancement theory and practice

    CERN Document Server

    Loizou, Philipos C

    2013-01-01

    With the proliferation of mobile devices and hearing devices, including hearing aids and cochlear implants, there is a growing and pressing need to design algorithms that can improve speech intelligibility without sacrificing quality. Responding to this need, Speech Enhancement: Theory and Practice, Second Edition introduces readers to the basic problems of speech enhancement and the various algorithms proposed to solve these problems. Updated and expanded, this second edition of the bestselling textbook broadens its scope to include evaluation measures and enhancement algorithms aimed at impr

  7. Enhanced Master Station History Report

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Enhanced Master Station History Report (EMSHR) is a compiled list of basic, historical information for every station in the station history database, beginning...

  8. [1990 annual waterfowl enhancement report

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — A memorandum from the Upper Souris NWR Refuge Manager to the regional office prefaces the attachment of the station's Annual Waterfowl Enhancement Report forms. The...

  9. Field enhancement induced laser ablation

    DEFF Research Database (Denmark)

    Fiutowski, Jacek; Maibohm, Christian; Kjelstrup-Hansen, Jakob

    Sub-diffraction spatially resolved, quantitative mapping of strongly localized field intensity enhancement on gold nanostructures via laser ablation of polymer thin films is reported. Illumination using a femtosecond laser scanning microscope excites surface plasmons in the nanostructures...

  10. Ultrasonic enhancement of battery diffusion.

    Science.gov (United States)

    Hilton, R; Dornbusch, D; Branson, K; Tekeei, A; Suppes, G J

    2014-03-01

    It has been demonstrated that sonic energy can be harnessed to enhance convection in Galvanic cells during cyclic voltammetry; however, the practical value of this approach is limited due to the lack of open volumes for convection patterns to develop in most batteries. This study evaluates the ability of ultrasonic waves to enhance diffusion in membrane separators commonly used in sandwich-architecture batteries. Studies include the measuring of open-circuit performance curves to interpret performances in terms of reductions in concentration overpotentials. The use of a 40 kHz sonicator bath can consistently increase the voltage of the battery and reduce overpotential losses up to 30%. This work demonstrates and quantifies battery enhancement due to enhanced diffusion made possible with ultrasonic energy.

  11. Enhanced Brine Dewatering System Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The purpose of the Enhanced Brine Dewatering System (EBDS) is to provide a scalable means of completely recovering usable water from byproducts created by reverse...

  12. Cognitive enhancement: a brief overview

    African Journals Online (AJOL)

    Keywords: ADHD, attention deficit/hyperactivity disorder, cognitive enhancement, high workload, narcolepsy, stress ... as effective and easier alternatives to more time-consuming ... reduce fatigue, thereby increasing training and/or learning.

  13. Moderate eugenics and human enhancement.

    Science.gov (United States)

    Selgelid, Michael J

    2014-02-01

    Though the reputation of eugenics has been tarnished by history, eugenics per se is not necessarily a bad thing. Many advocate a liberal new eugenics--where individuals are free to choose whether or not to employ genetic technologies for reproductive purposes. Though genetic interventions aimed at the prevention of severe genetic disorders may be morally and socially acceptable, reproductive liberty in the context of enhancement may conflict with equality. Enhancement could also have adverse effects on utility. The enhancement debate requires a shift in focus. What the equality and/or utility costs of enhancement will be is an empirical question. Rather than philosophical speculation, more social science research is needed to address it. Philosophers, meanwhile, should address head-on the question of how to strike a balance between liberty, equality, and utility in cases of conflict (in the context of genetics).

  14. Geothermal Permeability Enhancement - Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Joe Beall; Mark Walters

    2009-06-30

    The overall objective is to apply known permeability enhancement techniques to reduce the number of wells needed and demonstrate the applicability of the techniques to other undeveloped or under-developed fields. The Enhanced Geothermal System (EGS) concept presented in this project enhances energy extraction from reduced permeability zones in the super-heated, vapor-dominated Aidlin Field of the The Geysers geothermal reservoir. Numerous geothermal reservoirs worldwide, over a wide temperature range, contain zones of low permeability which limit the development potential and the efficient recovery of heat from these reservoirs. Low permeability results from poorly connected fractures or the lack of fractures. The Enhanced Geothermal System concept presented here expands these technologies by applying and evaluating them in a systematic, integrated program.

  15. Enhanced Brine Dewatering System Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The purpose of the Enhanced Brine Dewatering System (EBDS) is to provide an easily scalable means of completely recovering usable water from byproducts created by...

  16. Non-pharmacological cognitive enhancement

    NARCIS (Netherlands)

    Dresler, M.; Sandberg, A.; Ohla, K.; Bublitz, C.; Trenado, C.; Mroczko-Wasowicz, A.; Kuhn, S.; Repantis, D.

    2013-01-01

    The term "cognitive enhancement" usually characterizes interventions in humans that aim to improve mental functioning beyond what is necessary to sustain or restore good health. While the current bioethical debate mainly concentrates on pharmaceuticals, according to the given characterization,

  17. Bose enhancement and the ridge

    Energy Technology Data Exchange (ETDEWEB)

    Altinoluk, Tolga [Departamento de Física de Partículas and IGFAE, Universidade de Santiago de Compostela, 15706 Santiago de Compostela, Galicia (Spain); Armesto, Néstor, E-mail: nestor.armesto@usc.es [Departamento de Física de Partículas and IGFAE, Universidade de Santiago de Compostela, 15706 Santiago de Compostela, Galicia (Spain); Beuf, Guillaume [Department of Physics, Ben-Gurion University of the Negev, Beer Sheva 84105 (Israel); Kovner, Alex [Physics Department, University of Connecticut, 2152 Hillside Road, Storrs, CT 06269-3046 (United States); Lublinsky, Michael [Department of Physics, Ben-Gurion University of the Negev, Beer Sheva 84105 (Israel)

    2015-12-17

    We point out that Bose enhancement in a hadronic wave function generically leads to correlations between produced particles. We show explicitly, by calculating the projectile density matrix in the Color Glass Condensate approach to high-energy hadronic collisions, that the Bose enhancement of gluons in the projectile leads to azimuthal collimation of long range rapidity correlations of the produced particles, the so-called ridge correlations.

  18. Multispectral Enhancement towards Digital Staining

    Directory of Open Access Journals (Sweden)

    Pinky A. Bautista

    2012-01-01

    Full Text Available Background: Digital staining can be considered as a special form of image enhancement wherein the concern is not only to increase the contrast between the background objects and objects of interest, but to also impart the colors that mark the objects’ unique reactions to a specific stain. In this paper, we extended the previously proposed multispectral enhancement methods such that the colors of the background pixels can also be changed.

  19. Bose enhancement and the ridge

    CERN Document Server

    Altinoluk, Tolga; Beuf, Guillaume; Kovner, Alex; Lublinsky, Michael

    2015-01-01

    We point out that Bose enhancement in a hadronic wave function generically leads to correlations between produced particles. We show explicitly, by calculating the projectile density matrix in the Color Glass Condensate approach to high-energy hadronic collisions, that the Bose enhancement of gluons in the projectile leads to azimuthal collimation of long range rapidity correlations of the produced particles, the so-called ridge correlations.

  20. Bose enhancement and the ridge

    Directory of Open Access Journals (Sweden)

    Tolga Altinoluk

    2015-12-01

    Full Text Available We point out that Bose enhancement in a hadronic wave function generically leads to correlations between produced particles. We show explicitly, by calculating the projectile density matrix in the Color Glass Condensate approach to high-energy hadronic collisions, that the Bose enhancement of gluons in the projectile leads to azimuthal collimation of long range rapidity correlations of the produced particles, the so-called ridge correlations.

  1. Contrast-enhanced endoscopic ultrasonography

    DEFF Research Database (Denmark)

    Reddy, Nischita K; Ioncica, Ana Maria; Saftoiu, Adrian;

    2011-01-01

    agents and their use in various endoscopic procedures in the gastrointestinal tract. Various applications of contrast-enhanced endoscopic ultrasonography include differentiating benign from malignant mediastinal lymphadenopathy, assessment of depth of invasion of esophageal, gastric and gall bladder...... sonography to depict tumor neovascularization can be increased by contrast agents. Contrast-enhanced harmonic imaging is a useful aid in identifying the tumor vasculature and studying pancreatic microperfusion. In the future, these techniques could potentially be used to quantify tumor perfusion, to assess...

  2. Chemically enhanced in situ recovery

    Energy Technology Data Exchange (ETDEWEB)

    Sale, T. [CH2M Hill, Denver, CO (United States); Pitts, M.; Wyatt, K. [Surtek, Inc., Golden, CO (United States)] [and others

    1996-08-01

    Chemically enhanced recovery is a promising alternative to current technologies for management of subsurface releases of organic liquids. Through the inclusion of surfactants, solvents, polymers, and/or alkaline agents to a waterflood, the transport of targeted organic compounds can be increased and rates of recovery enhanced. By far, the vast majority of work done in the field of chemically enhanced recovery has been at a laboratory scale. The following text focuses on chemically enhanced recovery from a field application perspective with emphasis given to chlorinated solvents in a low permeability setting. While chlorinated solvents are emphasized, issues discussed are also relevant to organic liquids less dense than water such as petroleum products. Topics reviewed include: (1) Description of technology; (2) General technology considerations; (3) Low permeability media considerations; (4) Cost and reliability considerations; (5) Commercial availability; and (6) Case histories. Through this paper an appreciation is developed of both the potential and limitations of chemically enhanced recovery. Excluded from the scope of this paper is the in situ destruction of organic compounds through processes such as chemical or biological oxidation, chemically enhanced recovery of inorganic compounds, and ex situ soil treatment processes. 11 refs., 2 figs., 1 tab.

  3. 77 FR 25463 - Applications for New Awards; Enhanced Assessment Instruments Grants Program-Enhanced Assessment...

    Science.gov (United States)

    2012-04-30

    ... Instruments Grants that yield significant research, methodologies, products, or tools specifically regarding... Applications for New Awards; Enhanced Assessment Instruments Grants Program--Enhanced Assessment Instruments...: Notice. Overview Information: Enhanced Assessment Instruments Grants Program--Enhanced...

  4. Electron turbulence and transport in large magnetic islands

    Science.gov (United States)

    Morton, Lucas

    2016-10-01

    Magnetic islands, observed in both reversed-field pinches (RFPs) and tokamaks, often display unexpected turbulence and transport characteristics. For the first time in an RFP, the high repetition rate Thomson scattering diagnostic on MST has captured a 2D image of the rotating electron temperature structure of a magnetic island in a single discharge. MHD modeling using edge magnetic signals implies a 16 cm wide m,n =1,6 tearing mode island which completely overlaps a 5.5 cm n =7 island (12 cm between island centers). The 3D field is partially chaotic, but still reflective of the n =6 island structure. The measured temperature structure matches the shape and location of the n =6 partially chaotic (or `remnant') island. Contrary to the usual assumption that islands have flat internal temperature, the electron temperature is peaked inside the remnant magnetic island due to ohmic heating. The temperature peaking implies a local effective perpendicular conductivity 10-40 m2/s inside the remnant island. This agrees quantitatively with an effective perpendicular conductivity of 16 m2/s estimated using the magnetic diffusion coefficient (evaluated at the electron mean free path) calculated from the modeled chaotic field. Statistical analysis of measurement ensembles with lower time resolution implies that remnant island heating is common in MST discharges. To investigate the role of turbulence near a magnetic island, the 2D structure of long-wavelength density turbulence has been mapped around a large applied static m,n =2,1 L-mode island in the DIII-D tokamak. The turbulence exhibits intriguing spatial structure. Fluctuations are enhanced several-fold (compared to the no-island case) on the inboard side of the X-point, but not on the outboard side of the X-point and are also reduced near the O-point. This work is supported by the NSF and US DOE under DE-FC02-04ER54698, and DE-FG02-89ER53296.

  5. The Misfortunes of Moral Enhancement.

    Science.gov (United States)

    Azevedo, Marco Antonio

    2016-10-01

    In Unfit for the Future, Ingmar Persson and Julian Savulescu present a sophisticated argument in defense of the imperative of moral enhancement. They claim that without moral enhancement, the future of humanity is seriously compromised. The possibility of ultimate harm, caused by a dreadful terrorist attack or by a final unpreventable escalation of the present environmental crisis aggravated by the availability of cognitive enhancement, makes moral enhancement a top priority. It may be considered optimistic to think that our present moral capabilities can be successfully improved by means of moral education, moral persuasion, and fear of punishment. So, without moral enhancement, drastic restrictions on human freedom would become the only alternative to prevent those dramatic potential outcomes. In this article, I will try to show that we still have reason to be less pessimistic and that Persson & Savulescu's arguments are fortunately unconvincing. © The Author 2016. Published by Oxford University Press, on behalf of the Journal of Medicine and Philosophy Inc. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  6. Enhanced sludge washing evaluation plan

    Energy Technology Data Exchange (ETDEWEB)

    Jensen, R.D.

    1994-09-01

    The Tank Waste Remediation System (TWRS) Program mission is to store, treat, and immobilize highly radioactive Hanford Site waste (current and future tank waste and the strontium/cesium capsules) in an environmentally sound, safe, and cost-effective manner. The scope of the TWRS Waste Pretreatment Program is to treat tank waste and separate that waste into HLW and LLW fractions and provide additional treatment as required to feed LLW and HLW immobilization facilities. Enhanced sludge washing was chosen as the baseline process for separating Hanford tank waste sludge. Section 1.0 briefly discusses the purpose of the evaluation plan and provides the background that led to the choice of enhanced sludge washing as the baseline process. Section 2.0 provides a brief summary of the evaluation plan details. Section 3.0 discusses, in some detail, the technical work planned to support the evaluation of enhanced sludge washing. Section 4.0 briefly discusses the potential important of policy issues to the evaluation. Section 5.0 discusses the methodology to be used in the evaluation process. Section 6.0 summarizes the milestones that have been defined to complete the enhanced sludge washing evaluation and provides a summary schedule to evaluate the performance of enhanced sludge washing. References are identified in Section 7.0, and additional schedule and milestone information is provided in the appendices.

  7. Piezoelectric enhancement under negative pressure

    Science.gov (United States)

    Kvasov, Alexander; McGilly, Leo J.; Wang, Jin; Shi, Zhiyong; Sandu, Cosmin S.; Sluka, Tomas; Tagantsev, Alexander K.; Setter, Nava

    2016-07-01

    Enhancement of ferroelectric properties, both spontaneous polarization and Curie temperature under negative pressure had been predicted in the past from first principles and recently confirmed experimentally. In contrast, piezoelectric properties are expected to increase by positive pressure, through polarization rotation. Here we investigate the piezoelectric response of the classical PbTiO3, Pb(Zr,Ti)O3 and BaTiO3 perovskite ferroelectrics under negative pressure from first principles and find significant enhancement. Piezoelectric response is then tested experimentally on free-standing PbTiO3 and Pb(Zr,Ti)O3 nanowires under self-sustained negative pressure, confirming the theoretical prediction. Numerical simulations verify that negative pressure in nanowires is the origin of the enhanced electromechanical properties. The results may be useful in the development of highly performing piezoelectrics, including lead-free ones.

  8. Human enhancement: The new eugenics.

    Science.gov (United States)

    Vizcarrondo, Felipe E

    2014-08-01

    Supporters of human enhancement through genetic and other reproductive technologies claim that the new liberal eugenics, based on science and individual consent differs from the old eugenics which was unscientific and coercive. Supporters claim it is the parent's moral obligation to produce the best children possible. At this time, a defective gene that is identified in an unborn child cannot be repaired. To prevent the manifestation of the undesirable trait the unborn child is destroyed. The arguments in support of human enhancement are based on an ethic of consequence that could allow for nearly any means as long as the desired end is reached. Medical enhancement may affect the parent-child family unit; the parents' love for the child may be conditioned on the expected results. The new eugenics, although based on science, continues to pursue the same goal as the old eugenics, the development of a superior individual and the elimination of those considered inferior.

  9. Moral enhancement requires multiple virtues.

    Science.gov (United States)

    Hughes, James J

    2015-01-01

    Some of the debates around the concept of moral enhancement have focused on whether the improvement of a single trait, such as empathy or intelligence, would be a good in general, or in all circumstances. All virtue theories, however, both secular and religious, have articulated multiple virtues that temper and inform one another in the development of a mature moral character. The project of moral enhancement requires a reengagement with virtue ethics and contemporary moral psychology to develop an empirically grounded model of the virtues and a fuller model of character development. Each of these virtues may be manipulable with electronic, psychopharmaceutical, and genetic interventions. A set of interdependent virtues is proposed, along with some of the research pointing to ways such virtues could be enhanced.

  10. Plasmon-enhanced UV photocatalysis

    Energy Technology Data Exchange (ETDEWEB)

    Honda, Mitsuhiro; Saito, Yuika, E-mail: yuika@ap.eng.osaka-u.ac.jp; Kawata, Satoshi [Department of Applied Physics, Osaka University, Suita, Osaka 565-0871 (Japan); Kumamoto, Yasuaki [Nanophotonics Laboratory, RIKEN, Wako, Saitama 351-0198 (Japan); Taguchi, Atsushi [Nanophotonics Laboratory, RIKEN, Wako, Saitama 351-0198 (Japan); Department of Mechanical Systems Engineering, School of Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588 (Japan)

    2014-02-10

    We report plasmonic nanoparticle enhanced photocatalysis on titanium dioxide (TiO{sub 2}) in the deep-UV range. Aluminum (Al) nanoparticles fabricated on TiO{sub 2} film increases the reaction rate of photocatalysis by factors as high as 14 under UV irradiation in the range of 260–340 nm. The reaction efficiency has been determined by measuring the decolorization rate of methylene blue applied on the TiO{sub 2} substrate. The enhancement of photocatalysis shows particle size and excitation wavelength dependence, which can be explained by the surface plasmon resonance of Al nanoparticles.

  11. Bibliometric-enhanced Information Retrieval

    CERN Document Server

    Mayr, Philipp; Larsen, Birger; Schaer, Philipp; Mutschke, Peter

    2013-01-01

    Bibliometric techniques are not yet widely used to enhance retrieval processes in digital libraries, although they offer value-added effects for users. In this workshop we will explore how statistical modelling of scholarship, such as Bradfordizing or network analysis of coauthorship network, can improve re-trieval services for specific communities, as well as for large, cross-domain col-lections. This workshop aims to raise awareness of the missing link between in-formation retrieval (IR) and bibliometrics/scientometrics and to create a common ground for the incorporation of bibliometric-enhanced services into retrieval at the digital library interface.

  12. Perturbations can enhance qauntum search

    CERN Document Server

    Bae, J; Bae, Joonwoo; Kwon, Younghun

    2003-01-01

    In general, a quantum algorithm wants to avoid decoherence or perturbation, since such factors may cause errors in the algorithm. In this letter, we will supply the answer to the interesting question: can the factors seemingly harmful to a quantum algorithm(for example, perturbations) enhance the algorithm? We show that some perturbations to the generalized quantum search Hamiltonian can reduce the running time and enhance the success probability. We also provide the narrow bound to the perturbation which can be beneficial to quantum search. In addition, we show that the error induced by a perturbation on the Farhi and Gutmann Hamiltonian can be corrected by another perturbation.

  13. Effect of alcohols and enhancers on permeation enhancement of ketorolac

    Directory of Open Access Journals (Sweden)

    Chandra Amrish

    2009-01-01

    Full Text Available A reservoir-type transdermal patch for the delivery of ketorolac was studied. The low permeability of the skin is the rate-limiting step for delivery of most of the drugs. Studies were carried out to investigate the effect of pH, alcohols, and chemical permeation enhancers on the in vitro permeation of ketorolac. The reservoir core of the transdermal patch was filled with the hydrogel of a nonionic polymer, methocel K 15 M (hydroxyl propyl methylcellulose, HPMC formulated at an optimized pH of 5.4. Enhanced in vitro permeation was achieved after the incorporation of the alcohols. Higher enhancement was produced by short-chain alcohols like ethanol and isopropyl alcohol (IPA. Propylene glycol (PG along with other alcohols, viz. n-propanol, n-butanol, and n-pentanol, lagged behind. An exponential rise in permeation was observed in flux with an increase in the concentration of IPA. At 25%w/w IPA concentration, the observed ketorolac flux was 18.04mg/cm 2 /h. Terpene containing eucalyptus oil was studied to determine its permeation enhancement capability. The increase in the concentration of eucalyptus oil enhanced the drug permeation and a maximum flux of 66.38 and 90.56mg/cm 2 /h was achieved at 10 and 15%w/w concentrations. The anti-inflammatory potential of the transdermal formulation was evaluated on a carrageenan-induced paw edema model, with 41.67% inhibition at 6 h. The skin irritation potential was evaluated by the Drazie test and the formulations prepared were found to be safe. The reservoir-type transdermal patch for the delivery of ketorolac appeared to be feasible for delivering ketorolac across the skin.

  14. Advances in heat transfer enhancement

    CERN Document Server

    Saha, Sujoy Kumar; Sundén, Bengt; Wu, Zan

    2016-01-01

    This Brief addresses the phenomena of heat transfer enhancement. A companion edition in the SpringerBrief Subseries on Thermal Engineering and Applied Science to three other monographs including “Critical Heat Flux in Flow Boiling in Microchannels,” this volume is idea for professionals, researchers, and graduate students concerned with electronic cooling.

  15. Dimensional enhancement of kinetic energies

    DEFF Research Database (Denmark)

    Schleich, W.P.; Dahl, Jens Peder

    2002-01-01

    Simple thermodynamics considers kinetic energy to be an extensive variable which is proportional to the number N of particles. We present a quantum state of N noninteracting particles for which the kinetic energy increases quadratically with N. This enhancement effect is tied to the quantum centr...

  16. Managing to enhance soil health

    Science.gov (United States)

    Healthy soils are critical for meeting current and future societal demands. Management strategies that protect the soil against erosion, build soil organic matter and promote nutrient cycling are ways to enhance soil health. Keeping soils covered and judicious use of agrochemicals are akin to us “hu...

  17. Genomic Signal Enhancement by Clustering

    Institute of Scientific and Technical Information of China (English)

    ZHENG Wei-Mou

    2003-01-01

    Weight matrix models for signal sequence motif are simple. A main limitation of the models is the assumption of independence between positions. Signal enhancement is achieved by taking the total likelihood as the objective function for maximization to cluster sequences into groups with different patterns. As an example, the initial and terminal signals for translation in rice genome are examined.

  18. Enhancing hydrogen spillover and storage

    Science.gov (United States)

    Yang, Ralph T.; Li, Yingwel; Lachawiec, Jr., Anthony J.

    2011-05-31

    Methods for enhancing hydrogen spillover and storage are disclosed. One embodiment of the method includes doping a hydrogen receptor with metal particles, and exposing the hydrogen receptor to ultrasonification as doping occurs. Another embodiment of the method includes doping a hydrogen receptor with metal particles, and exposing the doped hydrogen receptor to a plasma treatment.

  19. Enhancing Access to Journal Literature.

    Science.gov (United States)

    Maine Univ., Orono. Raymond H. Fogler Library.

    The libraries of the University of Maine System, Bates, Bowdoin, and Colby Colleges have installed integrated systems which are being linked electronically and are designed to promote the maximum sharing of library resources, thereby significantly enhancing the teaching and research capabilities of each institution. The institutions propose to…

  20. Surface-enhanced Raman nanodomes

    Energy Technology Data Exchange (ETDEWEB)

    Choi, Charles J; Xu Zhida; Wu, Hsin-Yu; Liu, Gang Logan; Cunningham, Brian T, E-mail: bcunning@illinois.edu [Department of Electrical and Computer Engineering, Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, 208 North Wright Street, Urbana, IL 61801 (United States)

    2010-10-15

    We demonstrate a surface-enhanced Raman scattering (SERS) substrate consisting of a closely spaced metal nanodome array fabricated on flexible plastic film. We used a low-cost, large-area replica molding process to produce a two-dimensional periodic array of cylinders that is subsequently overcoated with SiO{sub 2} and silver thin films to form dome-shaped structures. Finite element modeling was used to investigate the electromagnetic field distribution of the nanodome array structure and the effect of the nanodome separation distance on the electromagnetic field enhancement. The SERS enhancement from the nanodome array substrates was experimentally verified using rhodamine 6G as the analyte. With a separation distance of 17 nm achieved between adjacent domes using a process that is precisely controlled during thin film deposition, a reproducible SERS enhancement factor of 1.37 x 10{sup 8} was demonstrated. The nanoreplica molding process presented in this work allows for simple, low-cost, high-throughput fabrication of uniform nanoscale SERS substrates over large surface areas without the requirement for high resolution lithography or defect-free deposition of spherical microparticle monolayer templates.

  1. Enhancements to PVM's BEOLIN architecture

    Science.gov (United States)

    Springer, Paul L.

    2005-01-01

    .4.3 of PVM had previously been enhanced by the addition of a new architecture, BEOLIN, which allowed a PVM user to abstract a Beowulf class computer with a private network to appear as a single system, visible to the outside world, which could spawn tasks on different internal nodes.

  2. Enhancing hydrogen spillover and storage

    Science.gov (United States)

    Yang, Ralph T; Li, Yingwei; Lachawiec, Jr., Anthony J

    2013-02-12

    Methods for enhancing hydrogen spillover and storage are disclosed. One embodiment of the method includes doping a hydrogen receptor with metal particles, and exposing the hydrogen receptor to ultrasonication as doping occurs. Another embodiment of the method includes doping a hydrogen receptor with metal particles, and exposing the doped hydrogen receptor to a plasma treatment.

  3. to enhance re-election

    African Journals Online (AJOL)

    PROF. O. E. OSUAGWU

    2013-12-01

    Dec 1, 2013 ... Assessing the Impact of Information Technology (WWW) on the attainment of Positive Corporate Image (PCI) to enhance re-election bid of incumbent ... public legitimacy and influence electoral victory at the polls. .... recognized main media and subdivision for ..... To avoid social and political conflicts,.

  4. Controlling plasmon-enhanced luminescence

    NARCIS (Netherlands)

    Mertens, H.

    2007-01-01

    Plasmons are collective oscillations of the free electrons in a metal or an ionized gas. Plasmons dominate the optical properties of noble-metal nanoparticles, which enables a variety of applications. This thesis focuses on plasmon-enhanced luminescence of silicon quantum dots (Si QDs) and optically

  5. CPS Science Laboratory Enhancement Project

    Energy Technology Data Exchange (ETDEWEB)

    James, Chandra

    2014-12-03

    The lab enhancement initiative was designed to support early implementation efforts of new policy to promote safe learning environments and school labs called the Chemical Safety and Hygiene Plan (CSHP). These efforts included comprehensive inventories and chemical removals at all Chicago Public High Schools, conducted by environmental health and safety consultants, and the development of professional development resources for teachers.

  6. Bubble chamber: colour enhanced tracks

    CERN Multimedia

    1998-01-01

    This artistically-enhanced image of real particle tracks was produced in the Big European Bubble Chamber (BEBC). Liquid hydrogen is used to create bubbles along the paths of the particles as a piston expands the medium. A magnetic field is produced in the detector causing the particles to travel in spirals, allowing charge and momentum to be measured.

  7. Progress in Diffraction Enhanced Imaging

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    @@ In cooperation with the Topography Station of Beijing Synchrotron Radiation under CAS Institute of High Energy Physics, a research group from the CAS Shanghai Institute of Optics and Fine Mechanics (SIOM) has made encouraging progress in the diffraction enhanced imaging technology through phase-contrast microscope by hard X-rays.

  8. Enhancing Author's Voice through Scripting

    Science.gov (United States)

    Young, Chase J.; Rasinski, Timothy V.

    2011-01-01

    The authors suggest using scripting as a strategy to mentor and enhance author's voice in writing. Through gradual release, students use authentic literature as a model for writing with voice. The authors also propose possible extensions for independent practice, integration across content areas, and tips for evaluation.

  9. Forensics: Enhancing Civic Literacy & Democracy

    Science.gov (United States)

    Briscoe, Shawn F.

    2009-01-01

    Forensics--interpretation, speech, and debate--can and should be a meaningful part of every school's curriculum. To put it simply, the course of study, alongside cocurricular competition, promotes civic education and enhances the standard curriculum by helping students explore myriad topics from multiple angles and find the truth in each,…

  10. Contrast enhancement in light microscopy.

    Science.gov (United States)

    Ernst Keller, H; Watkins, Simon

    2013-01-01

    The optical microscope is a fundamental component of an image cytometry system. This unit covers the basic concepts of light microscopy, including Köhler illumination, resolution, contrast, and numerical aperture, and reviews the many types of instruments and techniques for contrast enhancement.

  11. Bibliometric-enhanced information retrieval

    NARCIS (Netherlands)

    Mayr, Philipp; Scharnhorst, Andrea; Larsen, Birger; Schaer, Philipp; Mutschke, Peter

    2014-01-01

    Bibliometric techniques are not yet widely used to enhance retrieval processes in digital libraries, although they offer value-added effects for users. In this workshop we will explore how statistical modelling of scholarship, such as Bradfordizing or network analysis of coauthorship network, can im

  12. Contrast-enhanced peripheral MRA

    DEFF Research Database (Denmark)

    Nielsen, Yousef W; Thomsen, Henrik S

    2012-01-01

    In the last decade contrast-enhanced magnetic resonance angiography (CE-MRA) has gained wide acceptance as a valuable tool in the diagnostic work-up of patients with peripheral arterial disease. This review presents current concepts in peripheral CE-MRA with emphasis on MRI technique and contrast...

  13. Subendometrial enhancement and peritumoral enhancement for assessing endometrial cancer on dynamic contrast enhanced MR imaging

    Energy Technology Data Exchange (ETDEWEB)

    Fujii, Shinya [Division of Radiology, Department of Pathophysiological and Therapeutic Science, Faculty of Medicine, Tottori University, Yonago (Japan); Department of Diagnostic Imaging and Nuclear Medicine, Graduate School of Medicine, Kyoto University, Kyoto (Japan); Kido, Aki, E-mail: akikido@kuhp.kyoto-u.ac.jp [Department of Diagnostic Imaging and Nuclear Medicine, Graduate School of Medicine, Kyoto University, Kyoto (Japan); Baba, Tsukasa [Departments of Gynecology and Obstetrics, Graduate School of Medicine, Kyoto University, Kyoto (Japan); Fujimoto, Koji; Daido, Sayaka [Department of Diagnostic Imaging and Nuclear Medicine, Graduate School of Medicine, Kyoto University, Kyoto (Japan); Matsumura, Noriomi; Konishi, Ikuo [Departments of Gynecology and Obstetrics, Graduate School of Medicine, Kyoto University, Kyoto (Japan); Togashi, Kaori [Department of Diagnostic Imaging and Nuclear Medicine, Graduate School of Medicine, Kyoto University, Kyoto (Japan)

    2015-04-15

    Highlights: •We have assessed the peritumoral enhancement (PTE), which mimics SEE on DCE. •We evaluated the diagnostic accuracy of SEE for the myometrial invasion and the frequency of PTE. •We assessed the relationship between these enhancements and important pathologic factors. •PTE Type 1 is the main factor causing the overestimation of myometrial invasion using SEE on DCE. •PTE Type 2 correlates the myometrial invasion and may play an important role in the diagnosis of LVSI. -- Abstract: Objectives: To evaluate the diagnostic accuracy of subendometrial enhancement (SEE) in assessing the myometrial invasion in endometrial cancer, the frequency and clinical significance of peritumoral enhancement (PTE) on dynamic contrast enhanced (DCE) imaging. Materials and methods: MR images of 147 patients with endometrial cancer were retrospectively analyzed for intact SEE and PTEs: Type 1, a focal early enhancement peritumorally, and Type 2, an irregular thin-layered early intense enhancement peritumorally. Two radiologists independently assessed intact SEE and PTEs on DCE imaging and compared the lesions by the presence and depth of myometrial invasion, grade, lymphovascular space involvement (LVSI), and lymph node metastasis. The relationship between SEE, PTEs, and each factor was analyzed using univariate and multivariate analyses. The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and diagnostic accuracy were calculated for SEE. Results: The sensitivity, specificity, PPV, NPV and diagnostic accuracy for myometrial invasion based on SEE disruption on DCE were 96.6%, 32.1–46.4%, 85.8–88.5%, 69.2–76.5%, and 84.4–87.1%. According to multivariate analysis, SEE significantly predicted myometrial invasion (p < 0.0001). PTE Type 2 significantly predicted myometrial invasion presence (p < 0.05) and depth (p < 0.01). Conclusion: Diagnosis of myometrial invasion only by using SEE might be difficult on DCE-MRI due to the

  14. Image enhancement method for fingerprint recognition system.

    Science.gov (United States)

    Li, Shunshan; Wei, Min; Tang, Haiying; Zhuang, Tiange; Buonocore, Michael

    2005-01-01

    Image enhancement plays an important role in Fingerprint Recognition System. In this paper fingerprint image enhancement method, a refined Gabor filter, is presented. This enhancement method can connect the ridge breaks, ensures the maximal gray values located at the ridge center and has the ability to compensate for the nonlinear deformations. The result shows it can improve the performance of image enhancement.

  15. Blob-enhanced reconstruction technique

    Science.gov (United States)

    Castrillo, Giusy; Cafiero, Gioacchino; Discetti, Stefano; Astarita, Tommaso

    2016-09-01

    A method to enhance the quality of the tomographic reconstruction and, consequently, the 3D velocity measurement accuracy, is presented. The technique is based on integrating information on the objects to be reconstructed within the algebraic reconstruction process. A first guess intensity distribution is produced with a standard algebraic method, then the distribution is rebuilt as a sum of Gaussian blobs, based on location, intensity and size of agglomerates of light intensity surrounding local maxima. The blobs substitution regularizes the particle shape allowing a reduction of the particles discretization errors and of their elongation in the depth direction. The performances of the blob-enhanced reconstruction technique (BERT) are assessed with a 3D synthetic experiment. The results have been compared with those obtained by applying the standard camera simultaneous multiplicative reconstruction technique (CSMART) to the same volume. Several blob-enhanced reconstruction processes, both substituting the blobs at the end of the CSMART algorithm and during the iterations (i.e. using the blob-enhanced reconstruction as predictor for the following iterations), have been tested. The results confirm the enhancement in the velocity measurements accuracy, demonstrating a reduction of the bias error due to the ghost particles. The improvement is more remarkable at the largest tested seeding densities. Additionally, using the blobs distributions as a predictor enables further improvement of the convergence of the reconstruction algorithm, with the improvement being more considerable when substituting the blobs more than once during the process. The BERT process is also applied to multi resolution (MR) CSMART reconstructions, permitting simultaneously to achieve remarkable improvements in the flow field measurements and to benefit from the reduction in computational time due to the MR approach. Finally, BERT is also tested on experimental data, obtaining an increase of the

  16. Enhancing who? Enhancing what? Ethics, bioethics, and transhumanism.

    Science.gov (United States)

    Koch, Tom

    2010-12-01

    Transhumanists advance a "posthuman" condition in which technological and genetic enhancements will transform humankind. They are joined in this goal by bioethicists arguing for genetic selection as a means of "enhancing evolution," improving if not also the species then at least the potential lives of future individuals. The argument of both, this paper argues, is a new riff on the old eugenics tune. As ever, it is done in the name of science and its presumed knowledge base. As ever, the result is destructive rather than instructive, bad faith promoted as high ideal. The paper concludes with the argument that species advancement is possible but in a manner thoroughly distinct from that advanced by either of these groups.

  17. Three dimensional equilibrium solutions for a current-carrying reversed-field pinch plasma with a close-fitting conducting shell

    Energy Technology Data Exchange (ETDEWEB)

    Koliner, J. J.; Boguski, J., E-mail: boguski@wisc.edu; Anderson, J. K.; Chapman, B. E.; Den Hartog, D. J.; Duff, J. R.; Goetz, J. A.; McGarry, M.; Morton, L. A.; Parke, E. [Department of Physics, University of Wisconsin-Madison, Madison, Wisconsin 53706 (United States); Cianciosa, M. R. [Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831 (United States); Hanson, J. D. [Department of Physics, Auburn University, Auburn, Alabama 36849 (United States); Brower, D. L.; Ding, W. X. [Department of Physics and Astronomy, University of California, Los Angeles, California 90095 (United States)

    2016-03-15

    In order to characterize the Madison Symmetric Torus (MST) reversed-field pinch (RFP) plasmas that bifurcate to a helical equilibrium, the V3FIT equilibrium reconstruction code was modified to include a conducting boundary. RFP plasmas become helical at a high plasma current, which induces large eddy currents in MST's thick aluminum shell. The V3FIT conducting boundary accounts for the contribution from these eddy currents to external magnetic diagnostic coil signals. This implementation of V3FIT was benchmarked against MSTFit, a 2D Grad-Shafranov solver, for axisymmetric plasmas. The two codes both fit B{sub θ} measurement loops around the plasma minor diameter with qualitative agreement between each other and the measured field. Fits in the 3D case converge well, with q-profile and plasma shape agreement between two distinct toroidal locking phases. Greater than 60% of the measured n = 5 component of B{sub θ} at r = a is due to eddy currents in the shell, as calculated by the conducting boundary model.

  18. Three dimensional equilibrium solutions for a current-carrying reversed-field pinch plasma with a close-fitting conducting shell

    Science.gov (United States)

    Koliner, J. J.; Cianciosa, M. R.; Boguski, J.; Anderson, J. K.; Hanson, J. D.; Chapman, B. E.; Brower, D. L.; Den Hartog, D. J.; Ding, W. X.; Duff, J. R.; Goetz, J. A.; McGarry, M.; Morton, L. A.; Parke, E.

    2016-03-01

    In order to characterize the Madison Symmetric Torus (MST) reversed-field pinch (RFP) plasmas that bifurcate to a helical equilibrium, the V3FIT equilibrium reconstruction code was modified to include a conducting boundary. RFP plasmas become helical at a high plasma current, which induces large eddy currents in MST's thick aluminum shell. The V3FIT conducting boundary accounts for the contribution from these eddy currents to external magnetic diagnostic coil signals. This implementation of V3FIT was benchmarked against MSTFit, a 2D Grad-Shafranov solver, for axisymmetric plasmas. The two codes both fit Bθ measurement loops around the plasma minor diameter with qualitative agreement between each other and the measured field. Fits in the 3D case converge well, with q-profile and plasma shape agreement between two distinct toroidal locking phases. Greater than 60% of the measured n = 5 component of Bθ at r = a is due to eddy currents in the shell, as calculated by the conducting boundary model.

  19. GPP Webinar: Solar Utilization in Higher Education Networking & Information Sharing Group: RFP, Contract, and Administrative Issues Discussion

    Science.gov (United States)

    This presentation from a Solar Utilization in Higher Education Networking and Information webinar covers contracts, Request for Proposals (RFPs), and administrative issues related to solar project development in the higher education sector.

  20. Enhancing OPAC Records for Discovery

    Directory of Open Access Journals (Sweden)

    Patrick Griffis

    2009-09-01

    Full Text Available This article proposes adding keywords and descriptors to the catalog records of electronic databases and media items to enhance their discovery. The authors contend that subject liaisons can add value to OPAC records and enhance discovery of electronic databases and media items by providing searchable keywords and resource descriptions. The authors provide an examination of OPAC records at their own library, which illustrates the disparity of useful keywords and descriptions within the notes field for media item records versus electronic database records. The authors outline methods for identifying useful keywords for indexing OPAC records of electronic databases. Also included is an analysis of the advantages of using Encore’s Community Tag and Community Review features to allow subject liaisons to work directly in the catalog instead of collaborating with cataloging staff

  1. Gestures Enhance Foreign Language Learning

    Directory of Open Access Journals (Sweden)

    Manuela Macedonia

    2012-11-01

    Full Text Available Language and gesture are highly interdependent systems that reciprocally influence each other. For example, performing a gesture when learning a word or a phrase enhances its retrieval compared to pure verbal learning. Although the enhancing effects of co-speech gestures on memory are known to be robust, the underlying neural mechanisms are still unclear. Here, we summarize the results of behavioral and neuroscientific studies. They indicate that the neural representation of words consists of complex multimodal networks connecting perception and motor acts that occur during learning. In this context, gestures can reinforce the sensorimotor representation of a word or a phrase, making it resistant to decay. Also, gestures can favor embodiment of abstract words by creating it from scratch. Thus, we propose the use of gesture as a facilitating educational tool that integrates body and mind.

  2. Enhanced responsivity resonant RF photodetectors.

    Science.gov (United States)

    Liu, R; Dev, S; Zhong, Y; Lu, R; Streyer, W; Allen, J W; Allen, M S; Wenner, B R; Gong, S; Wasserman, D

    2016-11-14

    The responsivity of room-temperature, semiconductor-based photodetectors consisting of resonant RF circuits coupled to microstrip buslines is investigated. The dependence of the photodetector response on the semiconductor material and RF circuit geometry is presented, as is the detector response as a function of the spatial position of the incident light. We demonstrate significant improvement in detector response by choice of photoconductive material, and for a given material, by positioning our optical signal to overlap with positions of RF field enhancement. Design of RF circuits with strong field enhancement are demonstrated to further improve detector response. The improved detector response demonstrated offers opportunities for applications in RF photonics, materials metrology, or single read-out multiplexed detector arrays.

  3. Personalization enhances learning anatomy terms.

    Science.gov (United States)

    Ginns, Paul; Fraser, Jennifer

    2010-01-01

    Personalizing computer-based instructional text with a conversational rather than formal style has been found to enhance learning substantially. An experimental study investigated whether a conversational style would enhance learning anatomy terminology from paper-based materials. Students were randomly assigned to experimental conditions, and hypotheses were tested with a multiple-choice test and self-report scales. Studying personalized materials led to better performance on a terminology test and lower mental effort during testing than studying non-personalized materials. However, groups did not differ on ratings of interest/enjoyment. These results extend previous research by demonstrating learning gains following personalization using paper-based materials. However, the lack of effect on interest/enjoyment self-reports raises questions about previous theorizing on the role of this variable.

  4. Quantum-Enhanced Machine Learning.

    Science.gov (United States)

    Dunjko, Vedran; Taylor, Jacob M; Briegel, Hans J

    2016-09-23

    The emerging field of quantum machine learning has the potential to substantially aid in the problems and scope of artificial intelligence. This is only enhanced by recent successes in the field of classical machine learning. In this work we propose an approach for the systematic treatment of machine learning, from the perspective of quantum information. Our approach is general and covers all three main branches of machine learning: supervised, unsupervised, and reinforcement learning. While quantum improvements in supervised and unsupervised learning have been reported, reinforcement learning has received much less attention. Within our approach, we tackle the problem of quantum enhancements in reinforcement learning as well, and propose a systematic scheme for providing improvements. As an example, we show that quadratic improvements in learning efficiency, and exponential improvements in performance over limited time periods, can be obtained for a broad class of learning problems.

  5. Microbial enhanced oil recovery (MEOR).

    Science.gov (United States)

    Brown, Lewis R

    2010-06-01

    Two-thirds of the oil ever found is still in the ground even after primary and secondary production. Microbial enhanced oil recovery (MEOR) is one of the tertiary methods purported to increase oil recovery. Since 1946 more than 400 patents on MEOR have been issued, but none has gained acceptance by the oil industry. Most of the literature on MEOR is from laboratory experiments or from field trials of insufficient duration or that lack convincing proof of the process. Several authors have made recommendations required to establish MEOR as a viable method to enhance oil recovery, and until these tests are performed, MEOR will remain an unproven concept rather than a highly desirable reality. Copyright 2010 Elsevier Ltd. All rights reserved.

  6. Renormalization algorithm with graph enhancement

    CERN Document Server

    Hübener, R; Hartmann, L; Dür, W; Plenio, M B; Eisert, J

    2011-01-01

    We present applications of the renormalization algorithm with graph enhancement (RAGE). This analysis extends the algorithms and applications given for approaches based on matrix product states introduced in [Phys. Rev. A 79, 022317 (2009)] to other tensor-network states such as the tensor tree states (TTS) and projected entangled pair states (PEPS). We investigate the suitability of the bare TTS to describe ground states, showing that the description of certain graph states and condensed matter models improves. We investigate graph-enhanced tensor-network states, demonstrating that in some cases (disturbed graph states and for certain quantum circuits) the combination of weighted graph states with tensor tree states can greatly improve the accuracy of the description of ground states and time evolved states. We comment on delineating the boundary of the classically efficiently simulatable states of quantum many-body systems.

  7. Quantum-Enhanced Machine Learning

    Science.gov (United States)

    Dunjko, Vedran; Taylor, Jacob M.; Briegel, Hans J.

    2016-09-01

    The emerging field of quantum machine learning has the potential to substantially aid in the problems and scope of artificial intelligence. This is only enhanced by recent successes in the field of classical machine learning. In this work we propose an approach for the systematic treatment of machine learning, from the perspective of quantum information. Our approach is general and covers all three main branches of machine learning: supervised, unsupervised, and reinforcement learning. While quantum improvements in supervised and unsupervised learning have been reported, reinforcement learning has received much less attention. Within our approach, we tackle the problem of quantum enhancements in reinforcement learning as well, and propose a systematic scheme for providing improvements. As an example, we show that quadratic improvements in learning efficiency, and exponential improvements in performance over limited time periods, can be obtained for a broad class of learning problems.

  8. Superhydrophobicity enhancement through substrate flexibility.

    Science.gov (United States)

    Vasileiou, Thomas; Gerber, Julia; Prautzsch, Jana; Schutzius, Thomas M; Poulikakos, Dimos

    2016-11-22

    Inspired by manifestations in nature, microengineering and nanoengineering of synthetic materials to achieve superhydrophobicity has been the focus of much work. Generally, hydrophobicity is enhanced through the combined effects of surface texturing and chemistry; being durable, rigid materials are the norm. However, many natural and technical surfaces are flexible, and the resulting effect on hydrophobicity has been largely ignored. Here, we show that the rational tuning of flexibility can work synergistically with the surface microtexture or nanotexture to enhance liquid repellency performance, characterized by impalement and breakup resistance, contact time reduction, and restitution coefficient increase. Reduction in substrate areal density and stiffness imparts immediate acceleration and intrinsic responsiveness to impacting droplets (∼350 × g), mitigating the collision and lowering the impalement probability by ∼60% without the need for active actuation. Furthermore, we exemplify the above discoveries with materials ranging from man-made (thin steel or polymer sheets) to nature-made (butterfly wings).

  9. Biosurfactant-enhanced soil bioremediation

    Energy Technology Data Exchange (ETDEWEB)

    Kosaric, N.; Lu, G.; Velikonja, J. [Univ. of Western Ontario, London, Ontario (Canada)

    1995-12-01

    Bioremediation of soil contaminated with organic chemicals is a viable alternative method for clean-up and remedy of hazardous waste sites. The final objective in this approach is to convert the parent toxicant into a readily biodegradable product which is harmless to human health and/or the environment. Biodegradation of hydrocarbons in soil can also efficiently be enhanced by addition or in-situ production of biosufactants. It was generally observed that the degradation time was shortened and particularly the adaptation time for the microbes. More data from our laboratories showed that chlorinated aromatic compounds, such as 2,4-dichlorophenol, a herbicide Metolachlor, as well as naphthalene are degraded faster and more completely when selected biosurfactants are added to the soil. More recent data demonstrated an enhanced biodegradation of heavy hydrocarbons in petrochemical sludges, and in contaminated oil when biosurfactants were present or were added prior to the biodegradation process.

  10. Photodetector with enhanced light absorption

    Science.gov (United States)

    Kane, James

    1985-01-01

    A photodetector including a light transmissive electrically conducting layer having a textured surface with a semiconductor body thereon. This layer traps incident light thereby enhancing the absorption of light by the semiconductor body. A photodetector comprising a textured light transmissive electrically conducting layer of SnO.sub.2 and a body of hydrogenated amorphous silicon has a conversion efficiency about fifty percent greater than that of comparative cells. The invention also includes a method of fabricating the photodetector of the invention.

  11. Technology Education Professional Enhancement Project

    Science.gov (United States)

    Hughes, Thomas A., Jr.

    1996-01-01

    The two goals of this project are: the use of integrative field of aerospace technology to enhance the content and instruction delivered by math, science, and technology teachers through the development of a new publication entitled NASA Technology Today, and to develop a rationale and structure for the study of technology, which establishes the foundation for developing technology education standards and programs of the future.

  12. Decision Rules for Enhanced Breakout.

    Science.gov (United States)

    1987-03-20

    347AD-A18 2753 DECISION RULES FOR ENHANCED GRERICOUT(U) MODERN TECNOLOGIES CORP DAYTON 0ON T MI MCCANN 20 MAR 87 MTC-TR-8883-02 BRMC-85-564-i F33615... information to develop a priori estimates of the cost to break-out specific spare parts. In addition, some recommendations were to be developed...implementing directives. Applicable AF and AFLC Regulations and Pamphlets were also reviewed to obtain information on costs and their estimated

  13. Enhanced heat transfer using nanofluids

    Science.gov (United States)

    Choi, Stephen U. S.; Eastman, Jeffrey A.

    2001-01-01

    This invention is directed to a method of and apparatus for enhancing heat transfer in fluids such as deionized water. ethylene glycol, or oil by dispersing nanocrystalline particles of substances such as copper, copper oxide, aluminum oxide, or the like in the fluids. Nanocrystalline particles are produced and dispersed in the fluid by heating the substance to be dispersed in a vacuum while passing a thin film of the fluid near the heated substance. The fluid is cooled to control its vapor pressure.

  14. Enhancing Soundtracks From Old Movies

    Science.gov (United States)

    Frazer, Robert E.

    1992-01-01

    Proposed system enhances soundtracks of old movies. Signal on optical soundtrack of film digitized and processed to reduce noise and improve quality; timing signals added, and signal recorded on compact disk. Digital comparator and voltage-controlled oscillator synchronizes speed of film-drive motor and compact disk motor. Frame-coded detector reads binary frame-identifying marks on film. Digital comparator generates error signal if marks on film do not match those on compact disk.

  15. Threshold enhancement of diphoton resonances

    Directory of Open Access Journals (Sweden)

    Aoife Bharucha

    2016-10-01

    Full Text Available We revisit a mechanism to enhance the decay width of (pseudo-scalar resonances to photon pairs when the process is mediated by loops of charged fermions produced near threshold. Motivated by the recent LHC data, indicating the presence of an excess in the diphoton spectrum at approximately 750 GeV, we illustrate this threshold enhancement mechanism in the case of a 750 GeV pseudoscalar boson A with a two-photon decay mediated by a charged and uncolored fermion having a mass at the 12MA threshold and a small decay width, <1 MeV. The implications of such a threshold enhancement are discussed in two explicit scenarios: i the Minimal Supersymmetric Standard Model in which the A state is produced via the top quark mediated gluon fusion process and decays into photons predominantly through loops of charginos with masses close to 12MA and ii a two Higgs doublet model in which A is again produced by gluon fusion but decays into photons through loops of vector-like charged heavy leptons. In both these scenarios, while the mass of the charged fermion has to be adjusted to be extremely close to half of the A resonance mass, the small total widths are naturally obtained if only suppressed three-body decay channels occur. Finally, the implications of some of these scenarios for dark matter are discussed.

  16. Dispersion-Enhanced Laser Gyroscope

    Science.gov (United States)

    Smith, David D.; Chang, Hongrok; Arissian, L.; Diels, J. C.

    2008-01-01

    We analyze the effect of a highly dispersive element placed inside a modulated optical cavity on the frequency and amplitude of the output modulation to determine the conditions for enhanced gyroscopic sensitivities. The element is treated as both a phase and amplitude filter, and the time-dependence of the cavity field is considered. Both atomic gases (two-level and multi-level) and optical resonators (single and coupled) are considered and compared as dispersive elements. We find that it is possible to simultaneously enhance the gyro scale factor sensitivity and suppress the dead band by using an element with anomalous dispersion that has greater loss at the carrier frequency than at the side-band frequencies, i.e., an element that simultaneously pushes and intensifies the perturbed cavity modes, e.g. a two-level absorber or an under-coupled optical resonator. The sensitivity enhancement is inversely proportional to the effective group index, becoming infinite at a group index of zero. However, the number of round trips required to reach a steady-state also becomes infinite when the group index is zero (or two). For even larger dispersions a steady-state cannot be achieved, and nonlinear dynamic effects such as bistability and periodic oscillations are predicted in the gyro response.

  17. Contrast-enhanced digital mammography

    Energy Technology Data Exchange (ETDEWEB)

    Dromain, Clarisse [Department of Radiology, Institut Gustave-Roussy, 39, rue Camille Desmoulins, 94805 Villejuif Cedex (France)], E-mail: dromain@igr.fr; Balleyguier, Corinne; Adler, Ghazal [Department of Radiology, Institut Gustave-Roussy, 39, rue Camille Desmoulins, 94805 Villejuif Cedex (France); Garbay, Jean Remi [Department of Surgery, Institut Gustave-Roussy, 39, rue Camille Desmoulins, 94805 Villejuif Cedex (France); Delaloge, Suzette [Department of Medicine, Institut Gustave-Roussy, 39, rue Camille Desmoulins, 94805 Villejuif Cedex (France)

    2009-01-15

    CEDM is a recent development of digital mammography using the intra-venous injection of an iodinated contrast agent in conjunction with a mammography examination. Two techniques have been developed to perform CEDM examinations: the temporal subtraction technique with acquisition of high-energy images before and after contrast medium injection and the dual energy technique with acquisition of a pair of low and high-energy images only after contrast medium injection. The temporal subtraction technique offered the possibility to analyze the kinetic curve of enhancement of breast lesions, similarly to breast MRI. The dual energy technique do not provide information about the kinetic of tumor enhancement but allows the acquisition of multiples views of the same breast or bilateral examination and is less sensitive to patient motion than temporal CEDM. Initial clinical experience has shown the ability of CEDM to map the distribution of neovasculature induced by cancer using mammography. Moreover, previous studies have shown a superiority of MX + CEDM, either for the assessment of the probability of malignancy than for BIRADS assessment comparing to MX alone. The potential clinical applications are the clarification of mammographically equivocal lesions, the detection of occult lesions on standard mammography, particularly in dense breast, the determination of the extent of disease, the assessment of recurrent disease and the monitoring of the response to chemotherapy. CEDM should result in a simple way to enhance the detection and the characterization of breast lesions.

  18. Lateral interactions and enhanced adsorption

    Science.gov (United States)

    Rikvold, Per Arne; Deakin, Mark R.

    1991-06-01

    We extend earlier work on the effects of lateral adsorbate-adsorbate interactions in systems with two different adsorbate species to consider in detail enhanced adsorption phenomena. We give a detailed explanation of the enhancement mechanism for a lattice-gas model in thermodynamic equilibrium, and provide explicit quantitative criteria which must be satisfied by the effective lateral interactions in systems exhibiting strong, intermediate, or weak enhancement behavior. It is the examination and understanding of the topological details of the ground-state and phase diagrams of the model that allow the formulation of these criteria. The theoretically obtained criteria are supported by precise numerical calculations (transfer-matrix with strip width six) of adsorption isotherms for a three-state lattice-gas model with nearest-neighbor interactions on a triangular lattice. The applicability of this theoretical framework is illustrated by an analysis of experimental adsorption isotherms for the electrochemical adsorption of naphthalene on copper and n-decylamine on nickel, previously obtained by Bockris et al. As suggested by Damaskin et al. we attribute the potential dependence of the organic coverage to the influence of coadsorbed hydrogen. We find that nonlinear least-squares fits of numerical lattice-gas isotherms to the experimental data produce good agreement between the experimental and numerical adsorption isotherms, as well as effective lattice-gas interaction energies consistent with independent estimates from the literature.

  19. Dispersion-enhanced laser gyroscope

    Science.gov (United States)

    Smith, David D.; Chang, Hongrok; Arissian, L.; Diels, J. C.

    2008-11-01

    We analyze the effect of a highly dispersive element placed inside a modulated optical cavity on the frequency and amplitude of the output modulation to determine the conditions for enhanced gyroscopic sensitivities. The element is treated as both a phase and amplitude filter, and the time dependence of the cavity field is considered. Both atomic gases (two level and multilevel) and optical resonators (single and coupled) are considered and compared as dispersive elements. We find that it is possible to simultaneously enhance the gyro scale factor sensitivity and suppress the dead band by using an element with anomalous dispersion that has greater loss at the carrier frequency than at the sideband frequencies, i.e., an element that simultaneously pushes and intensifies the perturbed cavity modes, e.g. a two-level absorber or an undercoupled optical resonator. The sensitivity enhancement is inversely proportional to the effective group index, becoming infinite at a group index of zero. However, the number of round trips required to reach a steady state also becomes infinite when the group index is zero (or two). For even larger dispersions a steady state cannot be achieved, and nonlinear dynamic effects such as bistability and periodic oscillations are predicted in the gyro response.

  20. Interaction Enhanced Imperialist Competitive Algorithms

    Directory of Open Access Journals (Sweden)

    Meng-Shiou Li

    2012-10-01

    Full Text Available Imperialist Competitive Algorithm (ICA is a new population-based evolutionary algorithm. It divides its population of solutions into several sub-populations, and then searches for the optimal solution through two operations: assimilation and competition. The assimilation operation moves each non-best solution (called colony in a sub-population toward the best solution (called imperialist in the same sub-population. The competition operation removes a colony from the weakest sub-population and adds it to another sub-population. Previous work on ICA focuses mostly on improving the assimilation operation or replacing the assimilation operation with more powerful meta-heuristics, but none focuses on the improvement of the competition operation. Since the competition operation simply moves a colony (i.e., an inferior solution from one sub-population to another sub-population, it incurs weak interaction among these sub-populations. This work proposes Interaction Enhanced ICA that strengthens the interaction among the imperialists of all sub-populations. The performance of Interaction Enhanced ICA is validated on a set of benchmark functions for global optimization. The results indicate that the performance of Interaction Enhanced ICA is superior to that of ICA and its existing variants.

  1. The myth of genetic enhancement.

    Science.gov (United States)

    Rosoff, Philip M

    2012-06-01

    The ongoing revolution in molecular genetics has led many to speculate that one day we will be able to change the expression or phenotype of numerous complex traits to improve ourselves in many different ways. The prospect of genetic enhancements has generated heated controversy, with proponents advocating research and implementation, with caution advised for concerns about justice, and critics tending to see the prospect of genetic enhancements as an assault on human freedom and human nature. Both camps base their arguments on the unquestioned assumption that the science will realize either their dreams or nightmares. In this paper, I show that their beliefs are based upon two fundamental mistakes. First, they are based upon an unwarranted reliance in a genetic determinism that takes for granted that the traits that we might most want to enhance, like intelligence, aggression, shyness, and even athletic ability, can be causally directed by specific genes. In so doing, character descriptions are reified to be concrete and discrete entities, in this case, genes. Second, they have accepted on faith that there is, or will be, a science to translate their hopes or worries into reality when, in fact, that is unlikely to occur because of the irreducible complexity of phenotypic expression.

  2. Proposal for Two Enhanced NTRU

    Directory of Open Access Journals (Sweden)

    Ahmed Tariq Sadiq

    2014-06-01

    Full Text Available Sound is very widely used in communication. In order to ensure secure communication a cryptographic data scheme is used. Secure sound is needed in many fields such as military, business, banking and electronic commerce. There is also an increasing demand for secured sound in network communication. Several symmetric and asymmetric algorithms are used for sound encryption. In this work, NTRU, the last in line public key cryptosystem is enhanced in two methods and used for encrypting sound files after converting the sound into text. In the proposed methods the message is encrypted one character at a time, since NTRU encrypts only prime numbers, thus 7 bits of each character is encrypted and the eighth bit is left without encryption. In method I NTRU algorithm is enhanced by adding the result obtained from calculating a mathematical equation of one variable to the message and then the resulted encrypted bit is fed-back and added to the next bit of the message in the next step; this procedure is repeated for the subsequent bits of the message. In method II NTRU algorithm is enhanced by adding the subsequent states of LFSR (Linear Feedback Shift Register to the subsequent bytes of the message. The proposed methods are tested on several sound files; the results show that the proposed methods I and II maintain approximately the same original method encryption and decryption time while generating more complex encryption.

  3. Plasmonics Enhanced Smartphone Fluorescence Microscopy

    KAUST Repository

    Wei, Qingshan

    2017-05-12

    Smartphone fluorescence microscopy has various applications in point-of-care (POC) testing and diagnostics, ranging from e.g., quantification of immunoassays, detection of microorganisms, to sensing of viruses. An important need in smartphone-based microscopy and sensing techniques is to improve the detection sensitivity to enable quantification of extremely low concentrations of target molecules. Here, we demonstrate a general strategy to enhance the detection sensitivity of a smartphone-based fluorescence microscope by using surface-enhanced fluorescence (SEF) created by a thin metal-film. In this plasmonic design, the samples are placed on a silver-coated glass slide with a thin spacer, and excited by a laser-diode from the backside through a glass hemisphere, generating surface plasmon polaritons. We optimized this mobile SEF system by tuning the metal-film thickness, spacer distance, excitation angle and polarization, and achieved ~10-fold enhancement in fluorescence intensity compared to a bare glass substrate, which enabled us to image single fluorescent particles as small as 50 nm in diameter and single quantum-dots. Furthermore, we quantified the detection limit of this platform by using DNA origami-based brightness standards, demonstrating that ~80 fluorophores per diffraction-limited spot can be readily detected by our mobile microscope, which opens up new opportunities for POC diagnostics and sensing applications in resource-limited-settings.

  4. Cyclosporine A enhances platelet aggregation.

    Science.gov (United States)

    Grace, A A; Barradas, M A; Mikhailidis, D P; Jeremy, J Y; Moorhead, J F; Sweny, P; Dandona, P

    1987-12-01

    In view of the reported increase in thromboembolic episodes following cyclosporine A (CyA) therapy, the effect of this drug on platelet aggregation and thromboxane A2 release was investigated. The addition of CyA, at therapeutic concentrations to platelet rich plasma from normal subjects in vitro was found to increase aggregation in response to adrenaline, collagen and ADP. Ingestion of CyA by healthy volunteers was also associated with enhanced platelet aggregation. The CyA-mediated enhancement of aggregation was further enhanced by the addition in vitro of therapeutic concentrations of heparin. Platelets from renal allograft recipients treated with CyA also showed hyperaggregability and increased thromboxane A2 release, which were most marked at "peak" plasma CyA concentration and less so at "trough" concentrations. Platelet hyperaggregability in renal allograft patients on long-term CyA therapy tended to revert towards normal following the replacement of CyA with azathioprine. Hypertensive patients with renal allografts on nifedipine therapy had normal platelet function and thromboxane release in spite of CyA therapy. These observations suggest that CyA-mediated platelet activation may contribute to the pathogenesis of the thromboembolic phenomena associated with the use of this drug. The increased release of thromboxane A2 (a vasoconstrictor) may also play a role in mediating CyA-related nephrotoxicity.

  5. Enhanced live cell imaging via photonic crystal enhanced fluorescence microscopy.

    Science.gov (United States)

    Chen, Weili; Long, Kenneth D; Yu, Hojeong; Tan, Yafang; Choi, Ji Sun; Harley, Brendan A; Cunningham, Brian T

    2014-11-21

    We demonstrate photonic crystal enhanced fluorescence (PCEF) microscopy as a surface-specific fluorescence imaging technique to study the adhesion of live cells by visualizing variations in cell-substrate gap distance. This approach utilizes a photonic crystal surface incorporated into a standard microscope slide as the substrate for cell adhesion, and a microscope integrated with a custom illumination source as the detection instrument. When illuminated with a monochromatic light source, angle-specific optical resonances supported by the photonic crystal enable efficient excitation of surface-confined and amplified electromagnetic fields when excited at an on-resonance condition, while no field enhancement occurs when the same photonic crystal is illuminated in an off-resonance state. By mapping the fluorescence enhancement factor for fluorophore-tagged cellular components between on- and off-resonance states and comparing the results to numerical calculations, the vertical distance of labelled cellular components from the photonic crystal substrate can be estimated, providing critical and quantitative information regarding the spatial distribution of the specific components of cells attaching to a surface. As an initial demonstration of the concept, 3T3 fibroblast cells were grown on fibronectin-coated photonic crystals with fluorophore-labelled plasma membrane or nucleus. We demonstrate that PCEF microscopy is capable of providing information about the spatial distribution of cell-surface interactions at the single-cell level that is not available from other existing forms of microscopy, and that the approach is amenable to large fields of view, without the need for coupling prisms, coupling fluids, or special microscope objectives.

  6. ATAMM enhancement and multiprocessor performance evaluation

    Science.gov (United States)

    Stoughton, John W.; Mielke, Roland R.; Som, Sukhamoy; Obando, Rodrigo; Malekpour, Mahyar R.; Jones, Robert L., III; Mandala, Brij Mohan V.

    1991-01-01

    ATAMM (Algorithm To Architecture Mapping Model) enhancement and multiprocessor performance evaluation is discussed. The following topics are included: the ATAMM model; ATAMM enhancement; ADM (Advanced Development Model) implementation of ATAMM; and ATAMM support tools.

  7. A Survey on Speech Enhancement Methodologies

    Directory of Open Access Journals (Sweden)

    Ravi Kumar. K

    2016-12-01

    Full Text Available Speech enhancement is a technique which processes the noisy speech signal. The aim of speech enhancement is to improve the perceived quality of speech and/or to improve its intelligibility. Due to its vast applications in mobile telephony, VOIP, hearing aids, Skype and speaker recognition, the challenges in speech enhancement have grown over the years. It is more challenging to suppress back ground noise that effects human communication in noisy environments like airports, road works, traffic, and cars. The objective of this survey paper is to outline the single channel speech enhancement methodologies used for enhancing the speech signal which is corrupted with additive background noise and also discuss the challenges and opportunities of single channel speech enhancement. This paper mainly focuses on transform domain techniques and supervised (NMF, HMM speech enhancement techniques. This paper gives frame work for developments in speech enhancement methodologies

  8. DENdb: database of integrated human enhancers

    KAUST Repository

    Ashoor, Haitham

    2015-09-05

    Enhancers are cis-acting DNA regulatory regions that play a key role in distal control of transcriptional activities. Identification of enhancers, coupled with a comprehensive functional analysis of their properties, could improve our understanding of complex gene transcription mechanisms and gene regulation processes in general. We developed DENdb, a centralized on-line repository of predicted enhancers derived from multiple human cell-lines. DENdb integrates enhancers predicted by five different methods generating an enriched catalogue of putative enhancers for each of the analysed cell-lines. DENdb provides information about the overlap of enhancers with DNase I hypersensitive regions, ChIP-seq regions of a number of transcription factors and transcription factor binding motifs, means to explore enhancer interactions with DNA using several chromatin interaction assays and enhancer neighbouring genes. DENdb is designed as a relational database that facilitates fast and efficient searching, browsing and visualization of information.

  9. Complementary surface charge for enhanced capacitive deionization

    NARCIS (Netherlands)

    Gao, X.; Porada, S.; Omosebi, A.; Liu, K.L.; Biesheuvel, P.M.; Landon, J.

    2016-01-01

    Commercially available activated carbon cloth electrodes are treated using nitric acid and ethylenediamine solutions, resulting in chemical surface charge enhanced carbon electrodes for capacitive deionization (CDI) applications. Surface charge enhanced electrodes are then configured in a CDI cel

  10. Integration of cognitive and moral enhancement

    Directory of Open Access Journals (Sweden)

    Rakić Vojin

    2012-01-01

    Full Text Available I will discuss four major perspectives on cognitive enhancement and morality: 1 cognitive enhancement is morally impermissible because humans are not supposed to alter what God has ordained or nature has shaped; 2 cognitive enhancement is our moral duty, because a cognitively upgraded human is a better human; 3 cognitive enhancement is morally permissible only if it is preceded by moral enhancement; 4 cognitive enhancement is morally permissible only if it is a means to moral enhancement. I shall argue that the first three perspectives are less cogent than the fourth. The fourth perspective integrates cognitive and moral enhancement into one whole. I will denote it by (C+M E. [Projekat Ministarstva nauke Republike Srbije, br. 41004 i 43007

  11. DENdb: database of integrated human enhancers.

    Science.gov (United States)

    Ashoor, Haitham; Kleftogiannis, Dimitrios; Radovanovic, Aleksandar; Bajic, Vladimir B

    2015-01-01

    Enhancers are cis-acting DNA regulatory regions that play a key role in distal control of transcriptional activities. Identification of enhancers, coupled with a comprehensive functional analysis of their properties, could improve our understanding of complex gene transcription mechanisms and gene regulation processes in general. We developed DENdb, a centralized on-line repository of predicted enhancers derived from multiple human cell-lines. DENdb integrates enhancers predicted by five different methods generating an enriched catalogue of putative enhancers for each of the analysed cell-lines. DENdb provides information about the overlap of enhancers with DNase I hypersensitive regions, ChIP-seq regions of a number of transcription factors and transcription factor binding motifs, means to explore enhancer interactions with DNA using several chromatin interaction assays and enhancer neighbouring genes. DENdb is designed as a relational database that facilitates fast and efficient searching, browsing and visualization of information. Database URL: http://www.cbrc.kaust.edu.sa/dendb/.

  12. Enhanced migration of seismic data

    Energy Technology Data Exchange (ETDEWEB)

    Carrion, P.M. (PPPG/UFBA, Instituto de Geociencias, Rua Gaetano Moura 123, Federacao, 40.210 Salvador, Bahia (BR))

    1990-10-01

    The so-called enhanced migration which uses diffraction tomography as the repair tool for correction of amplitudes (reflection coefficients) of migrated sections is discussed. As with any linearized procedure, diffraction tomography requires knowledge of the initial model. It is suggested that the initial model is taken as the migrated image. It will be demonstrated that diffraction tomography applied to the data residuals improves the amplitudes of the migrated images. Migration is redefined as the reconstruction of the wavefront sets of distributions (reflection interfaces), and the inversion process as tomographic correction of migrated images.

  13. Does Design Rationale Enhance Creativity?

    Directory of Open Access Journals (Sweden)

    Jing Wang

    2010-01-01

    Full Text Available Creativity and rationale are often viewed as two contrasting facets in software design. A lack in recognizing the facilitative relationship between creativity and rationale not only underestimates the benefits designers can obtain from rationale practices, but also confines the approaches that support creativity in software design. Our exploratory study provides empirical evidence of the positive correlation between rationale and creativity. Furthermore, we found that the feasibility of design alternatives and the comprehensiveness of tradeoff evaluation are critical to enhancing novelty, persuasiveness, and insightfulness. We also discuss future directions to further understand how these properties, or rationale quality in general, affects design creativity.

  14. Enhanced Master Controller Unit Tester

    Science.gov (United States)

    Benson, Patricia; Johnson, Yvette; Johnson, Brian; Williams, Philip; Burton, Geoffrey; McCoy, Anthony

    2007-01-01

    The Enhanced Master Controller Unit Tester (EMUT) software is a tool for development and testing of software for a master controller (MC) flight computer. The primary function of the EMUT software is to simulate interfaces between the MC computer and external analog and digital circuitry (including other computers) in a rack of equipment to be used in scientific experiments. The simulations span the range of nominal, off-nominal, and erroneous operational conditions, enabling the testing of MC software before all the equipment becomes available.

  15. Glucose biosensor enhanced by nanoparticles

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Glucose biosensors have been formed with glucose oxidase (GOD) immobilized in composite immobilization membrane matrix, which is composed of hydrophobic gold, or hydrophilic gold, or hydrophobic silica nanoparticles, or the combination of gold and silica nanoparticles, and polyvinyl butyral (PVB) by a sol-gel method. The experiments show that nanoparticles can significantly enhance the catalytic activity of the immobilization enzyme. The current response can be increased from tens of nanoamperometer (nA) to thousands of nanoamperometer to the same glucose concentration, and the electrodes respond very quickly, to about 1 min. The function of nanoparticles effect on immobilization enzyme has been discussed.

  16. Glucose biosensor enhanced by nanoparticles

    Institute of Scientific and Technical Information of China (English)

    唐芳琼; 孟宪伟; 陈东; 冉均国; 郑昌琼

    2000-01-01

    Glucose biosensors have been formed with glucose oxidase (GOD) immobilized in composite immobilization membrane matrix, which is composed of hydrophobic gold, or hydro-philic gold, or hydrophobic silica nanoparticles, or the combination of gold and silica nanoparticles, and polyvinyl butyral (PVB) by a sol-gel method. The experiments show that nanoparticles can significantly enhance the catalytic activity of the immobilization enzyme. The current response can be increased from tens of nanoamperometer (nA) to thousands of nanoamperometer to the same glucose concentration, and the electrodes respond very quickly, to about 1 min. The function of nanoparticles effect on immobilization enzyme has been discussed.

  17. Enhancing the crisis management briefing.

    Science.gov (United States)

    Clark, Daniel W; Volkmann, Peter

    2005-01-01

    The Crisis Management Briefing (CMB) is a powerful and versatile crisis intervention tool, effective within a wide variety of populations, settings, and critical incidents. Initially proposed by Everly (2000), the CMB uniquely fills the factual information void following a critical incident. Additionally, a CMB provides essential information on effective coping strategies and available helping resources, tailored to the specific incident(s). The authors expand on the original CMB construct using their combined field experience, offering recommendations on team composition, strategic planning factors, using CISDs vs. CMBs, and specific enhancements for the four phases. They conclude with examples of specific interventions across a variety of events to illustrate their recommendations.

  18. Mutual Workshops enhancing Curriculum Integration

    DEFF Research Database (Denmark)

    Bjerregaard Jensen, Lotte; Markvorsen, Steen; Almegaard, Henrik

    2011-01-01

    -semester project in structural design was realized for the first time in autumn 2009. This paper presents the lessons learned from this first round along with the changes they inspired. Amongst the biggest changes made was the introduction of a successful joint workshop between the geometry course and the design...... course. This realized the full potential of structural design and firmly highlighted the creative potential in geometry for hesitant students. The joint workshop also showed potential as a general tool that can enhance curriculum integration....

  19. Directing a Quality Enhancement Plan

    Directory of Open Access Journals (Sweden)

    Ronald A. Styron, Jr.

    2015-08-01

    Full Text Available This paper is drawn from a project aimed at enhancing the quality of instructional practice and improving studentlearning outcomes in classes across an urban university located in the southern region of the United States. The strategies, employed by the project leader, are discussed in detail as framed by a new problem-solving leadership design called the Alloy Improvement Model (AIM. Data collected at the end of the second full year and reported in this paper indicated all project goals were met and that the utilization of the AIM was central to project success.

  20. The Enhanced Speed Bag System

    Science.gov (United States)

    2016-03-18

    system for helicopter resupply is a crucial development in aerial sustainment .  By Capt. Jude G.B. Coe Three enhanced speed bag systems are rigged on...NI NG & ED UC AT IO N 47 Army Sustainment July–August 2015 multipurpose cargo bag with pad- ding, one speed line assembly with a cable grip that...understand- ing of how to set up and rig the ESBS inside a Black Hawk. Validation Exercise After two joint in-progress reviews , Task Force Talon and the Iron

  1. Enhancing chaoticity of spatiotemporal chaos.

    Science.gov (United States)

    Li, Xiaowen; Zhang, Heqiao; Xue, Yu; Hu, Gang

    2005-01-01

    In some practical situations strong chaos is needed. This introduces the task of chaos control with enhancing chaoticity rather than suppressing chaoticity. In this paper a simple method of linear amplifications incorporating modulo operations is suggested to make spatiotemporal systems, which may be originally chaotic or nonchaotic, strongly chaotic. Specifically, this control can eliminate periodic windows, increase the values and the number of positive Lyapunov exponents, make the probability distributions of the output chaotic sequences more homogeneous, and reduce the correlations of chaotic outputs for different times and different space units. The applicability of the method to practical tasks, in particular to random number generators and secure communications, is briefly discussed.

  2. Gauge symmetry enhancement in Hamiltonian formalism

    CERN Document Server

    Hong, S T; Lee, T H; Oh, P; Oh, Phillial

    2003-01-01

    We study the Hamiltonian structure of the gauge symmetry enhancement in the enlarged CP(N) model coupled with U(2) chern-Simons term, which contains a free parameter governing explicit symmetry breaking and symmetry enhancement. After giving a general discussion of the geometry of constrained phase space suitable for the symmetry enhancement, we explicitly perform the Dirac analysis of out model and compute the Dirac brackets for the symmetry enhanced and broken cases. We also discuss some related issues.

  3. Resolution enhancement of color video sequences.

    Science.gov (United States)

    Shah, N R; Zakhor, A

    1999-01-01

    We propose a new multiframe algorithm to enhance the spatial resolution of frames in video sequences. Our technique specifically accounts for the possibility that motion estimation will be inaccurate and compensates for these inaccuracies. Experiments show that our multiframe enhancement algorithm yields perceptibly sharper enhanced images with significant signal-to-noise ratio (SNR) improvement over bilinear and cubic B-spline interpolation.

  4. Transcriptional enhancer from milk protein genes

    Energy Technology Data Exchange (ETDEWEB)

    Casperson, Gerald F. (Ballwin, MO); Schmidhauser, Christian T. (Berkeley, CA); Bissell, Mina J. (Berkeley, CA)

    1999-01-01

    The invention relates to novel enhancer nucleotide sequences which stimulate transcription of heterologous DNA in cells in culture. The enhancers are derived from major milk protein genes by the process of deletion mapping and functional analysis. The invention also relates to expression vectors containing the novel enhancers.

  5. Transcriptional enhancer from milk protein genes

    Energy Technology Data Exchange (ETDEWEB)

    Casperson, G.F.; Schmidhauser, C.T.; Bissell, M.J.

    1999-12-21

    The invention relates to novel enhancer nucleotide sequences which stimulate transcription of heterologous DNA in cells in culture. The enhancers are derived from major milk protein genes by the process of deletion mapping and functional analysis. The invention also relates to expression vectors containing the novel enhancers.

  6. Enhanced multifunctional paint for detection of radiation

    Energy Technology Data Exchange (ETDEWEB)

    Farmer, Joseph C.; Moses, Edward Ira; Rubenchik, Alexander M.

    2017-03-07

    An enhanced multifunctional paint apparatus, systems, and methods for detecting radiation on a surface include providing scintillation particles; providing an enhance neutron absorptive material; providing a binder; combining the scintillation particles, the enhance neutron absorptive material, and the binder creating a multifunctional paint; applying the multifunctional paint to the surface; and monitoring the surface for detecting radiation.

  7. Interpersonal leveling, independence, and self-enhancement

    DEFF Research Database (Denmark)

    Thomsen, Lotte; Sidanius, Jim; Fiske, Alan Page

    2007-01-01

    in self-enhancement. However, Danes practiced interpersonal leveling, preferring equality of outcome more than did Americans. This leveling strongly and inversely predicted self-enhancement within both cultures and mediated Danish-American differences in self-enhancement. In contrast, no independence...

  8. Threshold enhancement of diphoton resonances

    Science.gov (United States)

    Bharucha, Aoife; Djouadi, Abdelhak; Goudelis, Andreas

    2016-10-01

    We revisit a mechanism to enhance the decay width of (pseudo-)scalar resonances to photon pairs when the process is mediated by loops of charged fermions produced near threshold. Motivated by the recent LHC data, indicating the presence of an excess in the diphoton spectrum at approximately 750 GeV, we illustrate this threshold enhancement mechanism in the case of a 750 GeV pseudoscalar boson A with a two-photon decay mediated by a charged and uncolored fermion having a mass at the 1/2MA threshold and a small decay width, photons predominantly through loops of charginos with masses close to 1/2MA and ii) a two Higgs doublet model in which A is again produced by gluon fusion but decays into photons through loops of vector-like charged heavy leptons. In both these scenarios, while the mass of the charged fermion has to be adjusted to be extremely close to half of the A resonance mass, the small total widths are naturally obtained if only suppressed three-body decay channels occur. Finally, the implications of some of these scenarios for dark matter are discussed.

  9. Defense mutualisms enhance plant diversification.

    Science.gov (United States)

    Weber, Marjorie G; Agrawal, Anurag A

    2014-11-18

    The ability of plants to form mutualistic relationships with animal defenders has long been suspected to influence their evolutionary success, both by decreasing extinction risk and by increasing opportunity for speciation through an expanded realized niche. Nonetheless, the hypothesis that defense mutualisms consistently enhance plant diversification across lineages has not been well tested due to a lack of phenotypic and phylogenetic information. Using a global analysis, we show that the >100 vascular plant families in which species have evolved extrafloral nectaries (EFNs), sugar-secreting organs that recruit arthropod mutualists, have twofold higher diversification rates than families that lack species with EFNs. Zooming in on six distantly related plant clades, trait-dependent diversification models confirmed the tendency for lineages with EFNs to display increased rates of diversification. These results were consistent across methodological approaches. Inference using reversible-jump Markov chain Monte Carlo (MCMC) to model the placement and number of rate shifts revealed that high net diversification rates in EFN clades were driven by an increased number of positive rate shifts following EFN evolution compared with sister clades, suggesting that EFNs may be indirect facilitators of diversification. Our replicated analysis indicates that defense mutualisms put lineages on a path toward increased diversification rates within and between clades, and is concordant with the hypothesis that mutualistic interactions with animals can have an impact on deep macroevolutionary patterns and enhance plant diversity.

  10. Is caffeine a cognitive enhancer?

    Science.gov (United States)

    Nehlig, Astrid

    2010-01-01

    The effects of caffeine on cognition were reviewed based on the large body of literature available on the topic. Caffeine does not usually affect performance in learning and memory tasks, although caffeine may occasionally have facilitatory or inhibitory effects on memory and learning. Caffeine facilitates learning in tasks in which information is presented passively; in tasks in which material is learned intentionally, caffeine has no effect. Caffeine facilitates performance in tasks involving working memory to a limited extent, but hinders performance in tasks that heavily depend on working memory, and caffeine appears to rather improve memory performance under suboptimal alertness conditions. Most studies, however, found improvements in reaction time. The ingestion of caffeine does not seem to affect long-term memory. At low doses, caffeine improves hedonic tone and reduces anxiety, while at high doses, there is an increase in tense arousal, including anxiety, nervousness, jitteriness. The larger improvement of performance in fatigued subjects confirms that caffeine is a mild stimulant. Caffeine has also been reported to prevent cognitive decline in healthy subjects but the results of the studies are heterogeneous, some finding no age-related effect while others reported effects only in one sex and mainly in the oldest population. In conclusion, it appears that caffeine cannot be considered a ;pure' cognitive enhancer. Its indirect action on arousal, mood and concentration contributes in large part to its cognitive enhancing properties.

  11. Pharmacokinetic enhancers in HIV therapeutics.

    Science.gov (United States)

    Larson, Kajal B; Wang, Kun; Delille, Cecile; Otofokun, Igho; Acosta, Edward P

    2014-10-01

    Maximal and durable viral load suppression is one of the most important goals of HIV therapy and is directly related to adequate drug exposure. Protease inhibitors (PIs), an important component of the antiretroviral armada, were historically associated with poor oral bioavailability and high pill burden. However, because the PIs are metabolized by cytochrome P450 (CYP) 3A enzymes, intentional inhibition of these enzymes leads to higher drug exposure, lower pill burden, and therefore simplified dosing schedules with this class of drug. This is the basis of pharmacokinetic enhancement. In HIV therapy, two pharmacokinetic enhancers or boosting agents are used: ritonavir and cobicistat. Both agents inhibit CYP3A4, with cobicistat being a more specific CYP inhibitor than ritonavir. Unlike ritonavir, cobicistat does not have antiretroviral activity. Cobicistat has been evaluated in clinical trials and was recently approved in the USA as a fixed-dose combination with the integrase inhibitor, elvitegravir and two nucleos(t)ide analogs. Additional studies are examining cobicistat in fixed-dose combinations with various PIs. In this review, we summarize current knowledge of these agents and clinically relevant drug regimens and ongoing trials. Studies with elvitegravir and the novel PI TMC319011 are also discussed.

  12. Photonic crystal enhanced cytokine immunoassay.

    Science.gov (United States)

    Mathias, Patrick C; Ganesh, Nikhil; Cunningham, Brian T

    2009-01-01

    Photonic crystal surfaces are demonstrated as a means for enhancing the detection sensitivity and resolution for assays that use a fluorescent tag to quantify the concentration of an analyte protein molecule in a liquid test sample. Computer modeling of the spatial distribution of resonantly coupled electromagnetic fields on the photonic crystal surface are used to estimate the magnitude of enhancement factor compared to performing the same fluorescent assay on a plain glass surface, and the photonic crystal structure is fabricated and tested to experimentally verify the performance using a sandwich immunoassay for the protein Tumor Necrosis Factor-alpha (TNF-alpha). The demonstrated photonic crystal fabrication method utilizes a nanoreplica molding technique that allows for large-area inexpensive fabrication of the structure in a format that is compatible with confocal microarray laser scanners. The signal-to-noise ratio for fluorescent spots on the photonic crystal is increased by at least five-fold relative to the glass slide, allowing a TNF-alpha concentration of 1.6 pg/ml to be distinguished from noise on a photonic crystal surface. In addition, the minimum quantitative limit of detection on the photonic crystal surface is one-third the limit on the glass slide - a decrease from 18 pg/ml to 6 pg/ml. The increased performance of the immunoassay allows for more accurate quantitation of physiologically relevant concentrations of TNF-alpha in a protein microarray format that can be expanded to multiple cytokines.

  13. Surfactant enhanced volumetric sweep efficiency

    Energy Technology Data Exchange (ETDEWEB)

    Harwell, J.H.; Scamehorn, J.F.

    1989-10-01

    Surfactant-enhanced waterflooding is a novel EOR method aimed to improve the volumetric sweep efficiencies in reservoirs. The technique depends upon the ability to induce phase changes in surfactant solutions by mixing with surfactants of opposite charge or with salts of appropriate type. One surfactant or salt solution is injected into the reservoir. It is followed later by injection of another surfactant or salt solution. The sequence of injections is arranged so that the two solutions do not mix until they are into the permeable regions well away from the well bore. When they mix at this point, by design they form a precipitate or gel-like coacervate phase, plugging this permeable region, forcing flow through less permeable regions of the reservoir, improving sweep efficiency. The selectivity of the plugging process is demonstrated by achieving permeability reductions in the high permeable regions of Berea sandstone cores. Strategies were set to obtain a better control over the plug placement and the stability of plugs. A numerical simulator has been developed to investigate the potential increases in oil production of model systems. Furthermore, the hardness tolerance of anionic surfactant solutions is shown to be enhanced by addition of monovalent electrolyte or nonionic surfactants. 34 refs., 32 figs., 8 tabs.

  14. NANOCOMPOSITE COATINGS WITH ENHANCED HARDNESS

    Institute of Scientific and Technical Information of China (English)

    J. Musil

    2005-01-01

    The article reviews the present state of the art in the magnetron sputtering of hart and superhard nanocomposite coatings. It is shown that there are (1) two groups of hard and superhard nanocomposites: (i) nc-MN/hard phase and (ii) nc-MN/soft phase, (2) three possible origins of the enhanced hardness: (i) dislocation-dominated plastic deformation, (ii) cohesive forces between atoms and (iii) nanostructure of materials, and (3) huge differences in the microstructure of single- and two-phase films. A main attention is devoted to the formation of nanocrystalline and/or X-ray amorphous films. Such films are created in a vicinity of transitions between (i)crystalline and amorphous phases, (ii) two crystalline phases of different chemical composition or (iii) two different preferred orientations of grains of the sane material from which the coating is composed. The existence of the last transition makes it possible to explain the enhanced hardness in single-phase films. The thermal stability and oxidation resistance of hard nanocomposite films is also shortly discussed.

  15. Surfactant Enhanced Electroremediation of Phenanthrene

    Institute of Scientific and Technical Information of China (English)

    佘鹏; 杨建刚; 等

    2003-01-01

    Removal of hydrophobic organic contaminants(HOCs) form soil of low permeability by electroremediation was investigated by using phenanthrene and kaolinite as a model system.Tween 80 was added into the purging solution in order to enhance the solubility of phenanthrene.The effects of pH on the adsorption of phenanthrene and Tween 80 on kaolinite and the magnitude of ζ-potential of kaolinite were examined,respectively.The effects of electric field strength indicated by electric current on the electroremediation behavior,including the pH of purging solution,the conductivity,phenanthrene concentration and flow rate of effluent,were experimentally investigated,repectively,In case of an electric field of 25mA applied for 72 hours,over 90% of phenanthrene was removed from 424g(dry mass)of kaolinite at an energy consumption of 0.148kW.h.The experimental results described in present study show that the addition of surfactant into purging solution greatly enhances the removel of HOCs by electroremediation.

  16. RTU Comparison Calculator Enhancement Plan

    Energy Technology Data Exchange (ETDEWEB)

    Miller, James D. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Wang, Weimin [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Katipamula, Srinivas [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2015-07-01

    Over the past two years, Department of Energy’s Building Technologies Office (BTO) has been investigating ways to increase the operating efficiency of the packaged rooftop units (RTUs) in the field. First, by issuing a challenge to the RTU manufactures to increase the integrated energy efficiency ratio (IEER) by 60% over the existing ASHRAE 90.1-2010 standard. Second, by evaluating the performance of an advanced RTU controller that reduces the energy consumption by over 40%. BTO has previously also funded development of a RTU comparison calculator (RTUCC). RTUCC is a web-based tool that provides the user a way to compare energy and cost savings for two units with different efficiencies. However, the RTUCC currently cannot compare savings associated with either the RTU Challenge unit or the advanced RTU controls retrofit. Therefore, BTO has asked PNNL to enhance the tool so building owners can compare energy and savings associated with this new class of products. This document provides the details of the enhancements that are required to support estimating energy savings from use of RTU challenge units or advanced controls on existing RTUs.

  17. RTU Comparison Calculator Enhancement Plan

    Energy Technology Data Exchange (ETDEWEB)

    Miller, James D.; Wang, Weimin; Katipamula, Srinivas

    2014-03-31

    Over the past two years, Department of Energy’s Building Technologies Office (BTO) has been investigating ways to increase the operating efficiency of the packaged rooftop units (RTUs) in the field. First, by issuing a challenge to the RTU manufactures to increase the integrated energy efficiency ratio (IEER) by 60% over the existing ASHRAE 90.1-2010 standard. Second, by evaluating the performance of an advanced RTU controller that reduces the energy consumption by over 40%. BTO has previously also funded development of a RTU comparison calculator (RTUCC). RTUCC is a web-based tool that provides the user a way to compare energy and cost savings for two units with different efficiencies. However, the RTUCC currently cannot compare savings associated with either the RTU Challenge unit or the advanced RTU controls retrofit. Therefore, BTO has asked PNNL to enhance the tool so building owners can compare energy and savings associated with this new class of products. This document provides the details of the enhancements that are required to support estimating energy savings from use of RTU challenge units or advanced controls on existing RTUs.

  18. Enhanced Video-Oculography System

    Science.gov (United States)

    Moore, Steven T.; MacDougall, Hamish G.

    2009-01-01

    A previously developed video-oculography system has been enhanced for use in measuring vestibulo-ocular reflexes of a human subject in a centrifuge, motor vehicle, or other setting. The system as previously developed included a lightweight digital video camera mounted on goggles. The left eye was illuminated by an infrared light-emitting diode via a dichroic mirror, and the camera captured images of the left eye in infrared light. To extract eye-movement data, the digitized video images were processed by software running in a laptop computer. Eye movements were calibrated by having the subject view a target pattern, fixed with respect to the subject s head, generated by a goggle-mounted laser with a diffraction grating. The system as enhanced includes a second camera for imaging the scene from the subject s perspective, and two inertial measurement units (IMUs) for measuring linear accelerations and rates of rotation for computing head movements. One IMU is mounted on the goggles, the other on the centrifuge or vehicle frame. All eye-movement and head-motion data are time-stamped. In addition, the subject s point of regard is superimposed on each scene image to enable analysis of patterns of gaze in real time.

  19. Electrostatically enhanced core separator system

    Energy Technology Data Exchange (ETDEWEB)

    Easom, B.H.; Smolensky, L.A.; Altman, R.F. [LSR Technologies, Inc., Acton, MA (United States)

    1997-12-31

    The Electrostatically Enhanced Core Separator (EECS) system employs the same design principles as the mechanical Core Separator system plus an electrostatic separation enhancing technique. The EECS system contains a special type of separator, the EECS element, a conventional solids collector and means for flow recirculation. In the EECS system solids separation and collection are accomplished in two different components. The EECS element acts as a separator, not as a collector so particles are not collected on its walls. This eliminates or at least mitigates the problems associated with reentrainment (due to high or low dust resistivity), seepage (due to gas flow below the precipitator plates and over the hoppers), sneakage (due to gas flow both above and below the precipitator plates), and rapping reentrainment. If the EECS separation efficiency is high enough, particles cannot leave the system with the process stream. They recirculate until they are extracted by the collector. As a result, the separation efficiency of the EECS element determines the efficiency of the system, even if the collector efficiency is relatively low. 8 refs., 3 figs.

  20. Contrast-enhanced endoscopic ultrasonography

    Institute of Scientific and Technical Information of China (English)

    Nischita K Reddy; Ana Maria Ioncic(a); Adrian S(a)ftoiu; Peter Vilmann; Manoop S Bhutani

    2011-01-01

    Contrast agents are increasingly being used to characterize the vasculature in an organ of interest,to better delineate benign from malignant pathology and to aid in staging and directing therapeutic procedures.We review the mechanisms of action of first,second and third generation contrast agents and their use in various endoscopic procedures in the gastrointestinal tract.Various applications of contrast-enhanced endoscopic ultrasonography include differentiating benign from malignant mediastinal lymphadenopathy,assessment of depth of invasion of esophageal,gastric and gall bladder cancers and visualization of the portal venous system and esophageal varices.In addition,contrast agents can be used to differentiate pancreatic lesions.The use of color Doppler further increases the ability to diagnose and differentiate various pancreatic malignancies.The sensitivity of power Doppler sonography to depict tumor neovascularization can be increased by contrast agents.Contrast-enhanced harmonic imaging is a useful aid in identifying the tumor vasculature and studying pancreatic microperfusion.In the future,these techniques could potentially be used to quantify tumor perfusion,to assess and monitor the efficacy of antiangiogenic agents,to assist targeted drug delivery and allow molecular imaging.