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Sample records for mouse barrel cortex

  1. Membrane potential correlates of sensory perception in mouse barrel cortex.

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    Sachidhanandam, Shankar; Sreenivasan, Varun; Kyriakatos, Alexandros; Kremer, Yves; Petersen, Carl C H

    2013-11-01

    Neocortical activity can evoke sensory percepts, but the cellular mechanisms remain poorly understood. We trained mice to detect single brief whisker stimuli and report perceived stimuli by licking to obtain a reward. Pharmacological inactivation and optogenetic stimulation demonstrated a causal role for the primary somatosensory barrel cortex. Whole-cell recordings from barrel cortex neurons revealed membrane potential correlates of sensory perception. Sensory responses depended strongly on prestimulus cortical state, but both slow-wave and desynchronized cortical states were compatible with task performance. Whisker deflection evoked an early (sensory response that was encoded through cell-specific reversal potentials. A secondary late (50-400 ms) depolarization was enhanced on hit trials compared to misses. Optogenetic inactivation revealed a causal role for late excitation. Our data reveal dynamic processing in the sensory cortex during task performance, with an early sensory response reliably encoding the stimulus and later secondary activity contributing to driving the subjective percept.

  2. Plasticity-Related Gene 1 Affects Mouse Barrel Cortex Function via Strengthening of Glutamatergic Thalamocortical Transmission

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    Unichenko, Petr; Kirischuk, Sergei; Yang, Jenq-Wei; Baumgart, Jan; Roskoden, Thomas; Schneider, Patrick; Sommer, Angela; Horta, Guilherme; Radyushkin, Konstantin; Nitsch, Robert; Vogt, Johannes; Luhmann, Heiko J.

    2016-01-01

    Plasticity-related gene-1 (PRG-1) is a brain-specific protein that modulates glutamatergic synaptic transmission. Here we investigated the functional role of PRG-1 in adolescent and adult mouse barrel cortex both in vitro and in vivo. Compared with wild-type (WT) animals, PRG-1-deficient (KO) mice showed specific behavioral deficits in tests assessing sensorimotor integration and whisker-based sensory discrimination as shown in the beam balance/walking test and sandpaper tactile discriminatio...

  3. Plasticity-Related Gene 1 Affects Mouse Barrel Cortex Function via Strengthening of Glutamatergic Thalamocortical Transmission.

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    Unichenko, Petr; Kirischuk, Sergei; Yang, Jenq-Wei; Baumgart, Jan; Roskoden, Thomas; Schneider, Patrick; Sommer, Angela; Horta, Guilherme; Radyushkin, Konstantin; Nitsch, Robert; Vogt, Johannes; Luhmann, Heiko J

    2016-07-01

    Plasticity-related gene-1 (PRG-1) is a brain-specific protein that modulates glutamatergic synaptic transmission. Here we investigated the functional role of PRG-1 in adolescent and adult mouse barrel cortex both in vitro and in vivo. Compared with wild-type (WT) animals, PRG-1-deficient (KO) mice showed specific behavioral deficits in tests assessing sensorimotor integration and whisker-based sensory discrimination as shown in the beam balance/walking test and sandpaper tactile discrimination test, respectively. At P25-31, spontaneous network activity in the barrel cortex in vivo was higher in KO mice compared with WT littermates, but not at P16-19. At P16-19, sensory evoked cortical responses in vivo elicited by single whisker stimulation were comparable in KO and WT mice. In contrast, at P25-31 evoked responses were smaller in amplitude and longer in duration in WT animals, whereas KO mice revealed no such developmental changes. In thalamocortical slices from KO mice, spontaneous activity was increased already at P16-19, and glutamatergic thalamocortical inputs to Layer 4 spiny stellate neurons were potentiated. We conclude that genetic ablation of PRG-1 modulates already at P16-19 spontaneous and evoked excitability of the barrel cortex, including enhancement of thalamocortical glutamatergic inputs to Layer 4, which distorts sensory processing in adulthood. © The Author 2016. Published by Oxford University Press.

  4. Synaptic molecular imaging in spared and deprived columns of mouse barrel cortex with array tomography.

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    Weiler, Nicholas C; Collman, Forrest; Vogelstein, Joshua T; Burns, Randal; Smith, Stephen J

    2014-01-01

    A major question in neuroscience is how diverse subsets of synaptic connections in neural circuits are affected by experience dependent plasticity to form the basis for behavioral learning and memory. Differences in protein expression patterns at individual synapses could constitute a key to understanding both synaptic diversity and the effects of plasticity at different synapse populations. Our approach to this question leverages the immunohistochemical multiplexing capability of array tomography (ATomo) and the columnar organization of mouse barrel cortex to create a dataset comprising high resolution volumetric images of spared and deprived cortical whisker barrels stained for over a dozen synaptic molecules each. These dataset has been made available through the Open Connectome Project for interactive online viewing, and may also be downloaded for offline analysis using web, Matlab, and other interfaces.

  5. CREB Regulates Experience-Dependent Spine Formation and Enlargement in Mouse Barrel Cortex

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    Annabella Pignataro

    2015-01-01

    Full Text Available Experience modifies synaptic connectivity through processes that involve dendritic spine rearrangements in neuronal circuits. Although cAMP response element binding protein (CREB has a key function in spines changes, its role in activity-dependent rearrangements in brain regions of rodents interacting with the surrounding environment has received little attention so far. Here we studied the effects of vibrissae trimming, a widely used model of sensory deprivation-induced cortical plasticity, on processes associated with dendritic spine rearrangements in the barrel cortex of a transgenic mouse model of CREB downregulation (mCREB mice. We found that sensory deprivation through prolonged whisker trimming leads to an increased number of thin spines in the layer V of related barrel cortex (Contra in wild type but not mCREB mice. In the barrel field controlling spared whiskers (Ipsi, the same trimming protocol results in a CREB-dependent enlargement of dendritic spines. Last, we demonstrated that CREB regulates structural rearrangements of synapses that associate with dynamic changes of dendritic spines. Our findings suggest that CREB plays a key role in dendritic spine dynamics and synaptic circuits rearrangements that account for new brain connectivity in response to changes in the environment.

  6. Astrocytes control GABAergic inhibition of neurons in the mouse barrel cortex

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    Benedetti, B; Matyash, V; Kettenmann, H

    2011-01-01

    Astrocytes in the barrel cortex respond with a transient Ca2+ increase to neuronal stimulation and this response is restricted to the stimulated barrel field. In the present study we suppressed the astrocyte response by dialysing these cells with the Ca2+ chelator BAPTA. Electrical stimulation triggered a depolarization in stellate or pyramidal ‘regular spiking’ neurons from cortex layer 4 and 2/3 and this response was augmented in amplitude and duration after astrocytes were dialysed with BAPTA. Combined blockade of GABAA and GABAB receptors mimicked the effect of BAPTA dialysis, while glutamate receptor blockers had no effect. Moreover, the frequency of spontaneous postsynaptic currents was increased after BAPTA dialysis. Outside the range of BAPTA dialysis astrocytes responded with a Ca2+ increase, but in contrast to control, the response was no longer restricted to one barrel field. Our findings indicate that astrocytes control neuronal inhibition in the barrel cortex. PMID:21224221

  7. Astrocytes control GABAergic inhibition of neurons in the mouse barrel cortex.

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    Benedetti, B; Matyash, V; Kettenmann, H

    2011-03-01

    Astrocytes in the barrel cortex respond with a transient Ca2+ increase to neuronal stimulation and this response is restricted to the stimulated barrel field. In the present study we suppressed the astrocyte response by dialysing these cells with the Ca2+ chelator BAPTA. Electrical stimulation triggered a depolarization in stellate or pyramidal ‘regular spiking' neurons from cortex layer 4 and 2/3 and this response was augmented in amplitude and duration after astrocytes were dialysed with BAPTA. Combined blockade of GABAA and GABAB receptors mimicked the effect of BAPTA dialysis, while glutamate receptor blockers had no effect. Moreover, the frequency of spontaneous postsynaptic currents was increased after BAPTA dialysis. Outside the range of BAPTA dialysis astrocytes responded with a Ca2+ increase, but in contrast to control, the response was no longer restricted to one barrel field. Our findings indicate that astrocytes control neuronal inhibition in the barrel cortex.

  8. High-resolution 2-deoxyglucose mapping of functional cortical columns in mouse barrel cortex.

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    McCasland, J S; Woolsey, T A

    1988-12-22

    Cortical columns associated with barrels in layer IV of the somatosensory cortex were characterized by high-resolution 2-deoxy-D-glucose (2DG) autoradiography in freely behaving mice. The method demonstrates a more exact match between columnar labeling and cytoarchitectonic barrel boundaries than previously reported. The pattern of cortical activation seen with stimulation of a single whisker (third whisker in the middle row of large hairs--C3) was compared with the patterns from two control conditions--normal animals with all whiskers present ("positive control")--and with all large whiskers clipped ("negative control"). Two types of measurements were made from 2DG autoradiograms of tangential cortical sections: 1) labeled cells were identified by eye and tabulated with a computer, and 2) grain densities were obtained automatically with a computer-controlled microscope and image processor. We studied the fine-grained patterns of 2DG labeling in a nine-barrel grid with the C3 barrel in the center. From the analysis we draw five major conclusions. 1. Approximately 30-40% of the total number of neurons in the C3 barrel column are activated when only the C3 whisker is stimulated. This is about twice the number of neurons labeled in the C3 column when all whiskers are stimulated and about ten times the number of neurons labeled when all large whiskers are clipped. 2. There is evidence for a vertical functional organization within a barrel-related whisker column which has smaller dimensions in the tangential direction than a barrel. There are densely labeled patches within a barrel which are unique to an individual cortex. The same patchy pattern is found in the appropriate regions of sections above and below the barrels through the full thickness of the cortex. This functional arrangement could be considered to be a "minicolumn" or more likely a group of "minicolumns" (Mountcastle: In G.M. Edelman and U.B. Mountcastle (eds): The Material Brain: Cortical Organization

  9. Correlations decrease with propagation of spiking activity in the mouse barrel cortex

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    Gayathri Nattar Ranganathan

    2011-05-01

    Full Text Available Propagation of suprathreshold spiking activity through neuronal populations is important for the function of the central nervous system. Neural correlations have an impact on cortical function particularly on the signaling of information and propagation of spiking activity. Therefore we measured the change in correlations as suprathreshold spiking activity propagated between recurrent neuronal networks of the mammalian cerebral cortex. Using optical methods we recorded spiking activity from large samples of neurons from two neural populations simultaneously. The results indicate that correlations decreased as spiking activity propagated from layer 4 to layer 2/3 in the rodent barrel cortex.

  10. Layer- and column-specific knockout of NMDA receptors in pyramidal neurons of the mouse barrel cortex.

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    Rachel Aronoff

    2007-11-01

    Full Text Available Viral vectors injected into the mouse brain offer the possibility for localized genetic modifications in a highly controlled manner. Lentivector injection into mouse neocortex transduces cells within a diameter of approximately 200µm, which closely matches the lateral scale of a column in barrel cortex. The depth and volume of the injection determines which cortical layer is transduced. Furthermore, transduced gene expression from the lentivector can be limited to predominantly pyramidal neurons by using a 1.3kb fragment of the αCaMKII promoter. This technique therefore allows genetic manipulation of a specific cell type in defined columns and layers of the neocortex. By expressing Cre recombinase from such a lentivector in gene-targeted mice carrying a floxed gene, highly specific genetic lesions can be induced. Here, we demonstrate the utility of this approach by specifically knocking out NMDA receptors (NMDARs in pyramidal neurons in the somatosensory barrel cortex of gene-targeted mice carrying floxed NMDAR 1 genes. Neurons transduced with lentivector encoding GFP and Cre recombinase exhibit not only reductions in NMDAR 1 mRNA levels, but reduced NMDAR-dependent currents and pairing-induced synaptic potentiation. This technique for knockout of NMDARs in a cell type, column- and layer-specific manner in the mouse somatosensory cortex may help further our understanding of the functional roles of NMDARs in vivo during sensory perception and learning.

  11. Double dissociation of spike timing-dependent potentiation and depression by subunit-preferring NMDA receptor antagonists in mouse barrel cortex.

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    Banerjee, Abhishek; Meredith, Rhiannon M; Rodríguez-Moreno, Antonio; Mierau, Susanna B; Auberson, Yves P; Paulsen, Ole

    2009-12-01

    Spike timing-dependent plasticity (STDP) is a strong candidate for an N-methyl-D-aspartate (NMDA) receptor-dependent form of synaptic plasticity that could underlie the development of receptive field properties in sensory neocortices. Whilst induction of timing-dependent long-term potentiation (t-LTP) requires postsynaptic NMDA receptors, timing-dependent long-term depression (t-LTD) requires the activation of presynaptic NMDA receptors at layer 4-to-layer 2/3 synapses in barrel cortex. Here we investigated the developmental profile of t-LTD at layer 4-to-layer 2/3 synapses of mouse barrel cortex and studied their NMDA receptor subunit dependence. Timing-dependent LTD emerged in the first postnatal week, was present during the second week and disappeared in the adult, whereas t-LTP persisted in adulthood. An antagonist at GluN2C/D subunit-containing NMDA receptors blocked t-LTD but not t-LTP. Conversely, a GluN2A subunit-preferring antagonist blocked t-LTP but not t-LTD. The GluN2C/D subunit requirement for t-LTD appears to be synapse specific, as GluN2C/D antagonists did not block t-LTD at horizontal cross-columnar layer 2/3-to-layer 2/3 synapses, which was blocked by a GluN2B antagonist instead. These data demonstrate an NMDA receptor subunit-dependent double dissociation of t-LTD and t-LTP mechanisms at layer 4-to-layer 2/3 synapses, and suggest that t-LTD is mediated by distinct molecular mechanisms at different synapses on the same postsynaptic neuron.

  12. Patchwork-Type Spontaneous Activity in Neonatal Barrel Cortex Layer 4 Transmitted via Thalamocortical Projections

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    Hidenobu Mizuno

    2018-01-01

    Full Text Available Summary: Establishment of precise neuronal connectivity in the neocortex relies on activity-dependent circuit reorganization during postnatal development; however, the nature of cortical activity during this period remains largely unknown. Using two-photon calcium imaging of the barrel cortex in vivo during the first postnatal week, we reveal that layer 4 (L4 neurons within the same barrel fire synchronously in the absence of peripheral stimulation, creating a “patchwork” pattern of spontaneous activity corresponding to the barrel map. By generating transgenic mice expressing GCaMP6s in thalamocortical axons, we show that thalamocortical axons also demonstrate the spontaneous patchwork activity pattern. Patchwork activity is diminished by peripheral anesthesia but is mostly independent of self-generated whisker movements. The patchwork activity pattern largely disappeared during postnatal week 2, as even L4 neurons within the same barrel tended to fire asynchronously. This spontaneous L4 activity pattern has features suitable for thalamocortical (TC circuit refinement in the neonatal barrel cortex. : By two-photon calcium imaging of layer 4 neurons and thalamocortical axon terminals in neonatal mouse barrel cortex, Mizuno et al. find a patchwork-like spontaneous activity pattern corresponding to the barrel map, which may be important for thalamocortical circuit maturation. Keywords: activity-dependent development, spontaneous activity, synchronized activity, barrel cortex, thalamocortical axons, neonates, in vivo calcium imaging, awake, single-cell labeling, whisker monitoring

  13. Orofacial Neuropathic Pain Leads to a Hyporesponsive Barrel Cortex with Enhanced Structural Synaptic Plasticity.

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    Thibault, Karine; Rivière, Sébastien; Lenkei, Zsolt; Férézou, Isabelle; Pezet, Sophie

    2016-01-01

    Chronic pain is a long-lasting debilitating condition that is particularly difficult to treat due to the lack of identified underlying mechanisms. Although several key contributing processes have been described at the level of the spinal cord, very few studies have investigated the supraspinal mechanisms underlying chronic pain. Using a combination of approaches (cortical intrinsic imaging, immunohistochemical and behavioural analysis), our study aimed to decipher the nature of functional and structural changes in a mouse model of orofacial neuropathic pain, focusing on cortical areas involved in various pain components. Our results show that chronic neuropathic orofacial pain is associated with decreased haemodynamic responsiveness to whisker stimulation in the barrel field cortex. This reduced functional activation is likely due to the increased basal neuronal activity (measured indirectly using cFos and phospho-ERK immunoreactivity) observed in several cortical areas, including the contralateral barrel field, motor and cingulate cortices. In the same animals, immunohistochemical analysis of markers for active pre- or postsynaptic elements (Piccolo and phospho-Cofilin, respectively) revealed an increased immunofluorescence in deep cortical layers of the contralateral barrel field, motor and cingulate cortices. These results suggest that long-lasting orofacial neuropathic pain is associated with exacerbated neuronal activity and synaptic plasticity at the cortical level.

  14. A radial map of multi-whisker correlation selectivity in the rat barrel cortex.

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    Estebanez, Luc; Bertherat, Julien; Shulz, Daniel E; Bourdieu, Laurent; Léger, Jean-François

    2016-11-21

    In the barrel cortex, several features of single-whisker stimuli are organized in functional maps. The barrel cortex also encodes spatio-temporal correlation patterns of multi-whisker inputs, but so far the cortical mapping of neurons tuned to such input statistics is unknown. Here we report that layer 2/3 of the rat barrel cortex contains an additional functional map based on neuronal tuning to correlated versus uncorrelated multi-whisker stimuli: neuron responses to uncorrelated multi-whisker stimulation are strongest above barrel centres, whereas neuron responses to correlated and anti-correlated multi-whisker stimulation peak above the barrel-septal borders, forming rings of multi-whisker synchrony-preferring cells.

  15. Functional diversity of supragranular GABAergic neurons in the barrel cortex

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    Luc J Gentet

    2012-08-01

    Full Text Available Although the neocortex forms a distributed system comprised of several functional areas, its vertical columnar organization is largely conserved across areas and species, suggesting the existence of a canonical neocortical microcircuit. In order to elucidate the principles governing the organization of such a cortical diagram, a detailed understanding of the dynamics binding different types of cortical neurons into a coherent algorithm is essential. Within this complex circuitry, GABAergic interneurons, while forming approximately only 15-20% of all cortical neurons, appear critical in maintaining a dynamic balance between excitation and inhibition. Despite their importance, cortical GABAergic neurons have not been extensively studied in vivo and their precise role in shaping the local microcircuit sensory response still remains to be determined. Their paucity, combined with their molecular, anatomical and physiological diversity, has made it difficult to even establish a consensual nomenclature.However, recent technological advances in microscopy and mouse genetics have fostered a renewed interest in neocortical interneurons by putting them within visible reach of experimenters. The anatomically well-defined whisker-to-barrel pathway of the rodent is particularly amenable to studies attempting to link cortical circuit dynamics to behavior. To each whisker corresponds a discrete cortical unit equivalent to a single column, specialized in the encoding and processing of the sensory information it receives. In this review, we will focus on the functional role that each subtype of supragranular GABAergic neuron embedded within such a single neocortical unit may play in shaping the dynamics of the local circuit during somatosensory integration.

  16. Beta 2-adrenergic receptors are colocalized and coregulated with whisker barrels in rat somatosensory cortex

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    Vos, P.; Kaufmann, D.; Hand, P.J.; Wolfe, B.B.

    1990-01-01

    Autoradiography has been used to visualize independently the subtypes of beta-adrenergic receptors in rat somatosensory cortex. Beta 2-adrenergic receptors, but not beta 1-adrenergic receptors colocalize with whisker barrels in this tissue. Thus, each whisker sends a specific multisynaptic pathway to the somatosensory cortex that can be histochemically visualized and only one subtype of beta-adrenergic receptor is specifically associated with this cortical representation. Additionally, neonatal lesion of any or all of the whisker follicles results in loss of the corresponding barrel(s) as shown by histochemical markers. This loss is paralleled by a similar loss in the organization of beta 2-adrenergic receptors in the somatosensory cortex. Other results indicate that these beta 2-adrenergic receptors are not involved in moment-to-moment signal transmission in this pathway and, additionally, are not involved in a gross way in the development of whisker-barrel array

  17. Increases in the numerical density of GAT-1 positive puncta in the barrel cortex of adult mice after fear conditioning.

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    Ewa Siucinska

    Full Text Available Three days of fear conditioning that combines tactile stimulation of a row of facial vibrissae (conditioned stimulus, CS with a tail shock (unconditioned stimulus, UCS expands the representation of "trained" vibrissae, which can be demonstrated by labeling with 2-deoxyglucose in layer IV of the barrel cortex. We have also shown that functional reorganization of the primary somatosensory cortex (S1 increases GABAergic markers in the hollows of "trained" barrels of the adult mouse. This study investigated how whisker-shock conditioning (CS+UCS affected the expression of puncta of a high-affinity GABA plasma membrane transporter GAT-1 in the barrel cortex of mice 24 h after associative learning paradigm. We found that whisker-shock conditioning (CS+UCS led to increase expression of neuronal and astroglial GAT-1 puncta in the "trained" row compared to controls: Pseudoconditioned, CS-only, UCS-only and Naïve animals. These findings suggest that fear conditioning specifically induces activation of systems regulating cellular levels of the inhibitory neurotransmitter GABA.

  18. Effects of Arousal on Mouse Sensory Cortex Depend on Modality

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    Daisuke Shimaoka

    2018-03-01

    Full Text Available Summary: Changes in arousal modulate the activity of mouse sensory cortex, but studies in different mice and different sensory areas disagree on whether this modulation enhances or suppresses activity. We measured this modulation simultaneously in multiple cortical areas by imaging mice expressing voltage-sensitive fluorescent proteins (VSFP. VSFP imaging estimates local membrane potential across large portions of cortex. We used temporal filters to predict local potential from running speed or from pupil dilation, two measures of arousal. The filters provided good fits and revealed that the effects of arousal depend on modality. In the primary visual cortex (V1 and auditory cortex (Au, arousal caused depolarization followed by hyperpolarization. In the barrel cortex (S1b and a secondary visual area (LM, it caused only hyperpolarization. In all areas, nonetheless, arousal reduced the phasic responses to trains of sensory stimuli. These results demonstrate diverse effects of arousal across sensory cortex but similar effects on sensory responses. : Shimaoka et al. use voltage-sensitive imaging to show that the effects of arousal on the mouse cortex are markedly different across areas and over time. In all the sensory areas studied, nonetheless, arousal reduced the phasic voltage responses to trains of sensory stimuli. Keywords: cerebral cortex, cortical state, locomotion, sensory processing, widefield imaging

  19. Learning-Dependent Plasticity of the Barrel Cortex Is Impaired by Restricting GABA-Ergic Transmission.

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    Posluszny, Anna; Liguz-Lecznar, Monika; Turzynska, Danuta; Zakrzewska, Renata; Bielecki, Maksymilian; Kossut, Malgorzata

    2015-01-01

    Experience-induced plastic changes in the cerebral cortex are accompanied by alterations in excitatory and inhibitory transmission. Increased excitatory drive, necessary for plasticity, precedes the occurrence of plastic change, while decreased inhibitory signaling often facilitates plasticity. However, an increase of inhibitory interactions was noted in some instances of experience-dependent changes. We previously reported an increase in the number of inhibitory markers in the barrel cortex of mice after fear conditioning engaging vibrissae, observed concurrently with enlargement of the cortical representational area of the row of vibrissae receiving conditioned stimulus (CS). We also observed that an increase of GABA level accompanied the conditioning. Here, to find whether unaltered GABAergic signaling is necessary for learning-dependent rewiring in the murine barrel cortex, we locally decreased GABA production in the barrel cortex or reduced transmission through GABAA receptors (GABAARs) at the time of the conditioning. Injections of 3-mercaptopropionic acid (3-MPA), an inhibitor of glutamic acid decarboxylase (GAD), into the barrel cortex prevented learning-induced enlargement of the conditioned vibrissae representation. A similar effect was observed after injection of gabazine, an antagonist of GABAARs. At the behavioral level, consistent conditioned response (cessation of head movements in response to CS) was impaired. These results show that appropriate functioning of the GABAergic system is required for both manifestation of functional cortical representation plasticity and for the development of a conditioned response.

  20. Late emergence of the vibrissa direction selectivity map in the rat barrel cortex.

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    Kremer, Yves; Léger, Jean-François; Goodman, Dan; Brette, Romain; Bourdieu, Laurent

    2011-07-20

    In the neocortex, neuronal selectivities for multiple sensorimotor modalities are often distributed in topographical maps thought to emerge during a restricted period in early postnatal development. Rodent barrel cortex contains a somatotopic map for vibrissa identity, but the existence of maps representing other tactile features has not been clearly demonstrated. We addressed the issue of the existence in the rat cortex of an intrabarrel map for vibrissa movement direction using in vivo two-photon imaging. We discovered that the emergence of a direction map in rat barrel cortex occurs long after all known critical periods in the somatosensory system. This map is remarkably specific, taking a pinwheel-like form centered near the barrel center and aligned to the barrel cortex somatotopy. We suggest that this map may arise from intracortical mechanisms and demonstrate by simulation that the combination of spike-timing-dependent plasticity at synapses between layer 4 and layer 2/3 and realistic pad stimulation is sufficient to produce such a map. Its late emergence long after other classical maps suggests that experience-dependent map formation and refinement continue throughout adult life.

  1. Brain-wide map of efferent projections from rat barrel cortex

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    Izabela M. Zakiewicz

    2014-02-01

    Full Text Available The somatotopically organized whisker barrel field of the rat primary somatosensory (S1 cortex is a commonly used model system for anatomical and physiological investigations of sensory processing. The neural connections of the barrel cortex have been extensively mapped. But most investigations have focused on connections to limited regions of the brain, and overviews in the literature of the connections across the brain thus build on a range of material from different laboratories, presented in numerous publications. Furthermore, given the limitations of the conventional journal article format, analyses and interpretations are hampered by lack of access to the underlying experimental data. New opportunities for analyses have emerged with the recent release of an online resource of experimental data consisting of collections of high-resolution images from 6 experiments in which anterograde tracers were injected in S1 whisker or forelimb representations. Building on this material, we have conducted a detailed analysis of the brain wide distribution of the efferent projections of the rat barrel cortex. We compare our findings with the available literature and reports accumulated in the Brain Architecture Management System (BAMS2 database. We report well-known and less known intracortical and subcortical projections of the barrel cortex, as well as distinct differences between S1 whisker and forelimb related projections. Our results correspond well with recently published overviews, but provide additional information about relative differences among S1 projection targets. Our approach demonstrates how collections of shared experimental image data are suitable for brain-wide analysis and interpretation of connectivity mapping data.

  2. Barrels XXIX: Barrels go Hollywood.

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    Evans, Mathew H; Brumberg, Joshua C

    2017-03-01

    Barrels XXIX brought together researchers focusing on the rodent barrel cortex and associated systems. The meeting revolved around three themes: thalamocortical interactions in motor control, touch in rodent, monkey, and humans, and the nature of the multisensory computations the brain makes. Over two days these topics were covered as well as many more presentations that focused on the physiology, behavior, and development of the rodent whisker-to-barrel cortex system.

  3. Developmental Changes in Sensory-Evoked Optical Intrinsic Signals in the Rat Barrel Cortex

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    Mikhail Sintsov

    2017-12-01

    Full Text Available Optical Intrinsic Signal imaging (OISi is a powerful technique for optical brain studies. OIS mainly reflects the hemodynamic response (HR and metabolism, but it may also involve changes in tissue light scattering (LS caused by transient cellular swelling in the active tissue. Here, we explored the developmental features of sensory-evoked OIS in the rat barrel cortex during the first 3 months after birth. Multispectral OISi revealed that two temporally distinct components contribute to the neonatal OIS: an early phase of LS followed by a late phase of HR. The contribution of LS to the early response was also evidenced by an increase in light transmission through the active barrel. The early OIS phase correlated in time and amplitude with the sensory-evoked electrophysiological response. Application of the Modified Beer-Lambert Law (MBLL to the OIS data revealed that HR during the early phase involved only a slight decrease in blood oxygenation without any change in blood volume. In contrast, HR during the late phase manifested an adult-like increase in blood volume and oxygenation. During development, the peak time of the delayed HR progressively shortened with age, nearly reaching the stimulus onset and overlapping with the early LS phase by the fourth postnatal week. Thus, LS contributes to the sensory-evoked OIS in the barrel cortex of rats at all ages, and it dominates the early OIS phase in neonatal rats due to delayed HR. Our results are also consistent with the delayed blood oxygen level dependent (BOLD signal in human preterm infants.

  4. Developmental switch in neurovascular coupling in the immature rodent barrel cortex.

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    Christoph M Zehendner

    Full Text Available Neurovascular coupling (NVC in the adult central nervous system (CNS is a mechanism that provides regions of the brain with more oxygen and glucose upon increased levels of neural activation. Hemodynamic changes that go along with neural activation evoke a blood oxygen level-dependent (BOLD signal in functional magnetic resonance imaging (fMRI that can be used to study brain activity non-invasively. A correct correlation of the BOLD signal to neural activity is pivotal to understand this signal in neuronal development, health and disease. However, the function of NVC during development is largely unknown. The rodent whisker-to-barrel cortex is an experimentally well established model to study neurovascular interdependences. Using extracellular multi-electrode recordings and laser-Doppler-flowmetry (LDF we show in the murine barrel cortex of postnatal day 7 (P7 and P30 mice in vivo that NVC undergoes a physiological shift during the first month of life. In the mature CNS it is well accepted that cortical sensory processing results in a rise in regional cerebral blood flow (rCBF. We show in P7 animals that rCBF decreases during prolonged multi-whisker stimulation and goes along with multi unit activity (MUA fatigue. In contrast at P30, MUA remains stable during repetitive stimulation and is associated with an increase in rCBF. Further we characterize in both age groups the responses in NVC to single sensory stimuli. We suggest that the observed shift in NVC is an important process in cortical development that may be of high relevance for the correct interpretation of brain activity e.g. in fMRI studies of the immature central nervous system (CNS.

  5. Molecular Correlates of Cortical Network Modulation by Long-Term Sensory Experience in the Adult Rat Barrel Cortex

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    Vallès, Astrid; Granic, Ivica; De Weerd, Peter; Martens, Gerard J. M.

    2014-01-01

    Modulation of cortical network connectivity is crucial for an adaptive response to experience. In the rat barrel cortex, long-term sensory stimulation induces cortical network modifications and neuronal response changes of which the molecular basis is unknown. Here, we show that long-term somatosensory stimulation by enriched environment…

  6. Spatial integration in mouse primary visual cortex

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    Vaiceliunaite, Agne; Erisken, Sinem; Franzen, Florian; Katzner, Steffen; Busse, Laura

    2013-01-01

    Responses of many neurons in primary visual cortex (V1) are suppressed by stimuli exceeding the classical receptive field (RF), an important property that might underlie the computation of visual saliency. Traditionally, it has proven difficult to disentangle the underlying neural circuits, including feedforward, horizontal intracortical, and feedback connectivity. Since circuit-level analysis is particularly feasible in the mouse, we asked whether neural signatures of spatial integration in ...

  7. Texture coarseness responsive neurons and their mapping in layer 2–3 of the rat barrel cortex in vivo

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    Garion, Liora; Dubin, Uri; Rubin, Yoav; Khateb, Mohamed; Schiller, Yitzhak; Azouz, Rony; Schiller, Jackie

    2014-01-01

    Texture discrimination is a fundamental function of somatosensory systems, yet the manner by which texture is coded and spatially represented in the barrel cortex are largely unknown. Using in vivo two-photon calcium imaging in the rat barrel cortex during artificial whisking against different surface coarseness or controlled passive whisker vibrations simulating different coarseness, we show that layer 2–3 neurons within barrel boundaries differentially respond to specific texture coarsenesses, while only a minority of neurons responded monotonically with increased or decreased surface coarseness. Neurons with similar preferred texture coarseness were spatially clustered. Multi-contact single unit recordings showed a vertical columnar organization of texture coarseness preference in layer 2–3. These findings indicate that layer 2–3 neurons perform high hierarchical processing of tactile information, with surface coarseness embodied by distinct neuronal subpopulations that are spatially mapped onto the barrel cortex. DOI: http://dx.doi.org/10.7554/eLife.03405.001 PMID:25233151

  8. Effects of Adaptation on Discrimination of Whisker Deflection Velocity and Angular Direction in a Model of the Barrel Cortex

    Directory of Open Access Journals (Sweden)

    Mainak J. Patel

    2018-06-01

    Full Text Available Two important stimulus features represented within the rodent barrel cortex are velocity and angular direction of whisker deflection. Each cortical barrel receives information from thalamocortical (TC cells that relay information from a single whisker, and TC input is decoded by barrel regular-spiking (RS cells through a feedforward inhibitory architecture (with inhibition delivered by cortical fast-spiking or FS cells. TC cells encode deflection velocity through population synchrony, while deflection direction is encoded through the distribution of spike counts across the TC population. Barrel RS cells encode both deflection direction and velocity with spike rate, and are divided into functional domains by direction preference. Following repetitive whisker stimulation, system adaptation causes a weakening of synaptic inputs to RS cells and diminishes RS cell spike responses, though evidence suggests that stimulus discrimination may improve following adaptation. In this work, I construct a model of the TC, FS, and RS cells comprising a single barrel system—the model incorporates realistic synaptic connectivity and dynamics and simulates both angular direction (through the spatial pattern of TC activation and velocity (through synchrony of the TC population spikes of a deflection of the primary whisker, and I use the model to examine direction and velocity selectivity of barrel RS cells before and after adaptation. I find that velocity and direction selectivity of individual RS cells (measured over multiple trials sharpens following adaptation, but stimulus discrimination using a simple linear classifier by the RS population response during a single trial (a more biologically meaningful measure than single cell discrimination over multiple trials exhibits strikingly different behavior—velocity discrimination is similar both before and after adaptation, while direction classification improves substantially following adaptation. This is the

  9. Spatial integration in mouse primary visual cortex.

    Science.gov (United States)

    Vaiceliunaite, Agne; Erisken, Sinem; Franzen, Florian; Katzner, Steffen; Busse, Laura

    2013-08-01

    Responses of many neurons in primary visual cortex (V1) are suppressed by stimuli exceeding the classical receptive field (RF), an important property that might underlie the computation of visual saliency. Traditionally, it has proven difficult to disentangle the underlying neural circuits, including feedforward, horizontal intracortical, and feedback connectivity. Since circuit-level analysis is particularly feasible in the mouse, we asked whether neural signatures of spatial integration in mouse V1 are similar to those of higher-order mammals and investigated the role of parvalbumin-expressing (PV+) inhibitory interneurons. Analogous to what is known from primates and carnivores, we demonstrate that, in awake mice, surround suppression is present in the majority of V1 neurons and is strongest in superficial cortical layers. Anesthesia with isoflurane-urethane, however, profoundly affects spatial integration: it reduces the laminar dependency, decreases overall suppression strength, and alters the temporal dynamics of responses. We show that these effects of brain state can be parsimoniously explained by assuming that anesthesia affects contrast normalization. Hence, the full impact of suppressive influences in mouse V1 cannot be studied under anesthesia with isoflurane-urethane. To assess the neural circuits of spatial integration, we targeted PV+ interneurons using optogenetics. Optogenetic depolarization of PV+ interneurons was associated with increased RF size and decreased suppression in the recorded population, similar to effects of lowering stimulus contrast, suggesting that PV+ interneurons contribute to spatial integration by affecting overall stimulus drive. We conclude that the mouse is a promising model for circuit-level mechanisms of spatial integration, which relies on the combined activity of different types of inhibitory interneurons.

  10. Feedforward motor information enhances somatosensory responses and sharpens angular tuning of rat S1 barrel cortex neurons.

    Science.gov (United States)

    Khateb, Mohamed; Schiller, Jackie; Schiller, Yitzhak

    2017-01-06

    The primary vibrissae motor cortex (vM1) is responsible for generating whisking movements. In parallel, vM1 also sends information directly to the sensory barrel cortex (vS1). In this study, we investigated the effects of vM1 activation on processing of vibrissae sensory information in vS1 of the rat. To dissociate the vibrissae sensory-motor loop, we optogenetically activated vM1 and independently passively stimulated principal vibrissae. Optogenetic activation of vM1 supra-linearly amplified the response of vS1 neurons to passive vibrissa stimulation in all cortical layers measured. Maximal amplification occurred when onset of vM1 optogenetic activation preceded vibrissa stimulation by 20 ms. In addition to amplification, vM1 activation also sharpened angular tuning of vS1 neurons in all cortical layers measured. Our findings indicated that in addition to output motor signals, vM1 also sends preparatory signals to vS1 that serve to amplify and sharpen the response of neurons in the barrel cortex to incoming sensory input signals.

  11. Behavioral Consequences of a Bifacial Map in the Mouse Somatosensory Cortex.

    Science.gov (United States)

    Tsytsarev, Vassiliy; Arakawa, Hiroyuki; Zhao, Shuxin; Chédotal, Alain; Erzurumlu, Reha S

    2017-07-26

    The whisker system is an important sensory organ with extensive neural representations in the brain of the mouse. Patterned neural modules (barrelettes) in the ipsilateral principal sensory nucleus of the trigeminal nerve (PrV) correspond to the whiskers. Axons of the PrV barrelette neurons cross the midline and confer the whisker-related patterning to the contralateral ventroposteromedial nucleus of the thalamus, and subsequently to the cortex. In this way, specific neural modules called barreloids and barrels in the contralateral thalamus and cortex represent each whisker. Partial midline crossing of the PrV axons, in a conditional Robo3 mutant ( Robo3 R3-5 cKO ) mouse line, leads to the formation of bilateral whisker maps in the ventroposteromedial, as well as the barrel cortex. We used voltage-sensitive dye optical imaging and somatosensory and motor behavioral tests to characterize the consequences of bifacial maps in the thalamocortical system. Voltage-sensitive dye optical imaging verified functional, bilateral whisker representation in the barrel cortex and activation of distinct cortical loci following ipsilateral and contralateral stimulation of the specific whiskers. The mutant animals were comparable with the control animals in sensorimotor tests. However, they showed noticeable deficits in all of the whisker-dependent or -related tests, including Y-maze exploration, horizontal surface approach, bridge crossing, gap crossing, texture discrimination, floating in water, and whisking laterality. Our results indicate that bifacial maps along the thalamocortical system do not offer a functional advantage. Instead, they lead to impairments, possibly due to the smaller size of the whisker-related modules and interference between the ipsilateral and contralateral whisker representations in the same thalamus and cortex. SIGNIFICANCE STATEMENT The whisker sensory system plays a quintessentially important role in exploratory behavior of mice and other nocturnal

  12. Insulin-Independent GABAA Receptor-Mediated Response in the Barrel Cortex of Mice with Impaired Met Activity.

    Science.gov (United States)

    Lo, Fu-Sun; Erzurumlu, Reha S; Powell, Elizabeth M

    2016-03-30

    Autism spectrum disorder (ASD) is a neurodevelopmental disorder caused by genetic variants, susceptibility alleles, and environmental perturbations. The autism associated geneMETtyrosine kinase has been implicated in many behavioral domains and endophenotypes of autism, including abnormal neural signaling in human sensory cortex. We investigated somatosensory thalamocortical synaptic communication in mice deficient in Met activity in cortical excitatory neurons to gain insights into aberrant somatosensation characteristic of ASD. The ratio of excitation to inhibition is dramatically increased due to decreased postsynaptic GABAAreceptor-mediated inhibition in the trigeminal thalamocortical pathway of mice lacking active Met in the cerebral cortex. Furthermore, in contrast to wild-type mice, insulin failed to increase GABAAreceptor-mediated response in the barrel cortex of mice with compromised Met signaling. Thus, lacking insulin effects may be a risk factor in ASD pathogenesis. A proposed common cause of neurodevelopmental disorders is an imbalance in excitatory neural transmission, provided by the glutamatergic neurons, and the inhibitory signals from the GABAergic interneurons. Many genes associated with autism spectrum disorders impair synaptic transmission in the expected cell type. Previously, inactivation of the autism-associated Met tyrosine kinase receptor in GABAergic interneurons led to decreased inhibition. In thus report, decreased Met signaling in glutamatergic neurons had no effect on excitation, but decimated inhibition. Further experiments indicate that loss of Met activity downregulates GABAAreceptors on glutamatergic neurons in an insulin independent manner. These data provide a new mechanism for the loss of inhibition and subsequent abnormal excitation/inhibition balance and potential molecular candidates for treatment or prevention. Copyright © 2016 the authors 0270-6474/16/363691-07$15.00/0.

  13. Real-Time Million-Synapse Simulation of Rat Barrel Cortex

    Directory of Open Access Journals (Sweden)

    Thomas eSharp

    2014-05-01

    Full Text Available Simulations of neural circuits are bounded in scale and speed by available computing resources, and particularly by the differences in parallelism and communication patterns between the brain and high-performance computers. SpiNNaker is a computer architecture designed to address this problem by emulating the structure and function of neural tissue, using very many low-power processors and an interprocessor communication mechanism inspired by axonal arbors. Here we demonstrate that thousand-processor SpiNNaker prototypes can simulate models of the rodent barrel system comprising fifty thousand neurons and fifty million synapses. We use the PyNN library to specify models, and the intrinsic features of Python to control experimental procedures and analysis. The models reproduce known thalamocortical response transformations, exhibit known, balanced dynamics of excitation and inhibition, and show a spatiotemporal spread of activity though the superficial cortical layers. These demonstrations are a significant step towards tractable simulations of entire cortical areas on the million-processor SpiNNaker machines in development.

  14. Real-time million-synapse simulation of rat barrel cortex.

    Science.gov (United States)

    Sharp, Thomas; Petersen, Rasmus; Furber, Steve

    2014-01-01

    Simulations of neural circuits are bounded in scale and speed by available computing resources, and particularly by the differences in parallelism and communication patterns between the brain and high-performance computers. SpiNNaker is a computer architecture designed to address this problem by emulating the structure and function of neural tissue, using very many low-power processors and an interprocessor communication mechanism inspired by axonal arbors. Here we demonstrate that thousand-processor SpiNNaker prototypes can simulate models of the rodent barrel system comprising 50,000 neurons and 50 million synapses. We use the PyNN library to specify models, and the intrinsic features of Python to control experimental procedures and analysis. The models reproduce known thalamocortical response transformations, exhibit known, balanced dynamics of excitation and inhibition, and show a spatiotemporal spread of activity though the superficial cortical layers. These demonstrations are a significant step toward tractable simulations of entire cortical areas on the million-processor SpiNNaker machines in development.

  15. Comparison of (stereotactic) parcellations in mouse prefrontal cortex

    NARCIS (Netherlands)

    van de Werd, H.J.J.M.; Uylings, H.B.M.

    2014-01-01

    This study compares the cytoarchitectonic parcellation of the prefrontal cortex (PFC) in the mouse as presented in publications that are commonly used for identifying brain areas. Agreement was found to be greater for boundaries in the medial PFC than in the lateral PFC and lowest for those in the

  16. Bidirectional Hebbian Plasticity Induced by Low-Frequency Stimulation in Basal Dendrites of Rat Barrel Cortex Layer 5 Pyramidal Neurons.

    Science.gov (United States)

    Díez-García, Andrea; Barros-Zulaica, Natali; Núñez, Ángel; Buño, Washington; Fernández de Sevilla, David

    2017-01-01

    According to Hebb's original hypothesis (Hebb, 1949), synapses are reinforced when presynaptic activity triggers postsynaptic firing, resulting in long-term potentiation (LTP) of synaptic efficacy. Long-term depression (LTD) is a use-dependent decrease in synaptic strength that is thought to be due to synaptic input causing a weak postsynaptic effect. Although the mechanisms that mediate long-term synaptic plasticity have been investigated for at least three decades not all question have as yet been answered. Therefore, we aimed at determining the mechanisms that generate LTP or LTD with the simplest possible protocol. Low-frequency stimulation of basal dendrite inputs in Layer 5 pyramidal neurons of the rat barrel cortex induces LTP. This stimulation triggered an EPSP, an action potential (AP) burst, and a Ca 2+ spike. The same stimulation induced LTD following manipulations that reduced the Ca 2+ spike and Ca 2+ signal or the AP burst. Low-frequency whisker deflections induced similar bidirectional plasticity of action potential evoked responses in anesthetized rats. These results suggest that both in vitro and in vivo similar mechanisms regulate the balance between LTP and LTD. This simple induction form of bidirectional hebbian plasticity could be present in the natural conditions to regulate the detection, flow, and storage of sensorimotor information.

  17. Bidirectional Hebbian Plasticity Induced by Low-Frequency Stimulation in Basal Dendrites of Rat Barrel Cortex Layer 5 Pyramidal Neurons

    Science.gov (United States)

    Díez-García, Andrea; Barros-Zulaica, Natali; Núñez, Ángel; Buño, Washington; Fernández de Sevilla, David

    2017-01-01

    According to Hebb's original hypothesis (Hebb, 1949), synapses are reinforced when presynaptic activity triggers postsynaptic firing, resulting in long-term potentiation (LTP) of synaptic efficacy. Long-term depression (LTD) is a use-dependent decrease in synaptic strength that is thought to be due to synaptic input causing a weak postsynaptic effect. Although the mechanisms that mediate long-term synaptic plasticity have been investigated for at least three decades not all question have as yet been answered. Therefore, we aimed at determining the mechanisms that generate LTP or LTD with the simplest possible protocol. Low-frequency stimulation of basal dendrite inputs in Layer 5 pyramidal neurons of the rat barrel cortex induces LTP. This stimulation triggered an EPSP, an action potential (AP) burst, and a Ca2+ spike. The same stimulation induced LTD following manipulations that reduced the Ca2+ spike and Ca2+ signal or the AP burst. Low-frequency whisker deflections induced similar bidirectional plasticity of action potential evoked responses in anesthetized rats. These results suggest that both in vitro and in vivo similar mechanisms regulate the balance between LTP and LTD. This simple induction form of bidirectional hebbian plasticity could be present in the natural conditions to regulate the detection, flow, and storage of sensorimotor information. PMID:28203145

  18. Efficacy and connectivity of intracolumnar pairs of layer 2/3 pyramidal cells in the barrel cortex of juvenile rats

    Science.gov (United States)

    Feldmeyer, Dirk; Lübke, Joachim; Sakmann, Bert

    2006-01-01

    Synaptically coupled layer 2/3 (L2/3) pyramidal neurones located above the same layer 4 barrel (‘barrel-related’) were investigated using dual whole-cell voltage recordings in acute slices of rat somatosensory cortex. Recordings were followed by reconstructions of biocytin-filled neurones. The onset latency of unitary EPSPs was 1.1 ± 0.4 ms, the 20–80% rise time was 0.7 ± 0.2 ms, the average amplitude was 1.0 ± 0.7 mV and the decay time constant was 15.7 ± 4.5 ms. The coefficient of variation (c.v.) of unitary EPSP amplitudes decreased with increasing EPSP peak and was 0.33 ± 0.18. Bursts of APs in the presynaptic pyramidal cell resulted in EPSPs that, over a wide range of frequencies (5–100 Hz), displayed amplitude depression. Anatomically the barrel-related pyramidal cells in the lower half of layer 2/3 have a long apical dendrite with a small terminal tuft, while pyramidal cells in the upper half of layer 2/3 have shorter and often more ‘irregularly’ shaped apical dendrites that branch profusely in layer 1. The number of putative excitatory synaptic contacts established by the axonal collaterals of a L2/3 pyramidal cell with a postsynaptic pyramidal cell in the same column varied between 2 and 4, with an average of 2.8 ± 0.7 (n = 8 pairs). Synaptic contacts were established predominantly on the basal dendrites at a mean geometric distance of 91 ± 47 μm from the pyramidal cell soma. L2/3-to-L2/3 connections formed a blob-like innervation domain containing 2.8 mm of the presynaptic axon collaterals with a bouton density of 0.3 boutons per μm axon. Within the supragranular layers of its home column a single L2/3 pyramidal cell established about 900 boutons suggesting that 270 pyramidal cells in layer 2/3 are innervated by an individual pyramidal cell. In turn, a single pyramidal cell received synaptic inputs from 270 other L2/3 pyramidal cells. The innervation domain of L2/3-to-L2/3 connections superimposes almost exactly with that of L4-to-L2

  19. Visual Information Present in Infragranular Layers of Mouse Auditory Cortex.

    Science.gov (United States)

    Morrill, Ryan J; Hasenstaub, Andrea R

    2018-03-14

    The cerebral cortex is a major hub for the convergence and integration of signals from across the sensory modalities; sensory cortices, including primary regions, are no exception. Here we show that visual stimuli influence neural firing in the auditory cortex of awake male and female mice, using multisite probes to sample single units across multiple cortical layers. We demonstrate that visual stimuli influence firing in both primary and secondary auditory cortex. We then determine the laminar location of recording sites through electrode track tracing with fluorescent dye and optogenetic identification using layer-specific markers. Spiking responses to visual stimulation occur deep in auditory cortex and are particularly prominent in layer 6. Visual modulation of firing rate occurs more frequently at areas with secondary-like auditory responses than those with primary-like responses. Auditory cortical responses to drifting visual gratings are not orientation-tuned, unlike visual cortex responses. The deepest cortical layers thus appear to be an important locus for cross-modal integration in auditory cortex. SIGNIFICANCE STATEMENT The deepest layers of the auditory cortex are often considered its most enigmatic, possessing a wide range of cell morphologies and atypical sensory responses. Here we show that, in mouse auditory cortex, these layers represent a locus of cross-modal convergence, containing many units responsive to visual stimuli. Our results suggest that this visual signal conveys the presence and timing of a stimulus rather than specifics about that stimulus, such as its orientation. These results shed light on both how and what types of cross-modal information is integrated at the earliest stages of sensory cortical processing. Copyright © 2018 the authors 0270-6474/18/382854-09$15.00/0.

  20. Subplate in the developing cortex of mouse and human

    DEFF Research Database (Denmark)

    Wang, Wei Zhi; Hoerder-Suabedissen, Anna; Oeschger, Franziska M

    2010-01-01

    Abstract The subplate is a largely transient zone containing precocious neurons involved in several key steps of cortical development. The majority of subplate neurons form a compact layer in mouse, but are dispersed throughout a much larger zone in the human. In rodent, subplate neurons are among...... several genes that are specifically expressed in the subplate layer of the rodent dorsal cortex. Here we examined the human subplate for some of these markers. In the human dorsal cortex, connective tissue growth factor-positive neurons can be seen in the ventricular zone at 15-22 postconceptional weeks...... growth factor- and nuclear receptor-related 1-positive cells are two distinct cell populations of the human subplate. Furthermore, our microarray analysis in rodent suggested that subplate neurons produce plasma proteins. Here we demonstrate that the human subplate also expresses alpha2zinc...

  1. Unique functional properties of somatostatin-expressing GABAergic neurons in mouse barrel cortex.

    NARCIS (Netherlands)

    Gentet, L.J.; Kremer, Y.; Taniguchi, H.; Huang, Z.J.; Staiger, J.F.; Petersen, C.C.H.

    2012-01-01

    Neocortical GABAergic neurons have diverse molecular, structural and electrophysiological features, but the functional correlates of this diversity are largely unknown. We found unique membrane potential dynamics of somatostatin-expressing (SOM) neurons in layer 2/3 of the primary somatosensory

  2. Effect of ionizing radiation on apoptosis in the cortex of mouse lymph node

    International Nuclear Information System (INIS)

    Chen Dong; Liu Jiamei; Liu Shuzheng

    1999-01-01

    Objective: To study the alteration of apoptosis in the cortex of mouse lymph node following whole body X-irradiation. Methods: The method of TdT-mediated dUTP nick end labelling (TUNEL) was used to detect apoptosis the cortex of mouse lymph node. Results: The sensitivity to high and low dose ionizing radiation was distinct in different area of the cortex. Conclusion: The decrease of apoptotic cells in the inter nodular and deep cortex indicate that low dose radiation may suppress the apoptosis of T lymphocytes and play a role in immune regulation

  3. Developmental changes in electrophysiological properties and a transition from electrical to chemical coupling between excitatory layer 4 neurons in the rat barrel cortex

    Directory of Open Access Journals (Sweden)

    Fliza eValiullina

    2016-01-01

    Full Text Available During development, sensory systems switch from an immature to an adult mode of function along with the emergence of the active cortical states. Here, we used patch-clamp recordings from neocortical slices in vitro to characterize the developmental changes in the basic electrophysiological properties of excitatory L4 neurons and their connectivity before and after the developmental switch, which occurs in the rat barrel cortex in vivo at postnatal day P8. Prior to the switch, L4 neurons had lower resting membrane potentials, higher input resistance, lower membrane capacity, as well as action potentials (APs with smaller amplitudes, longer durations and higher AP thresholds compared to the neurons after the switch. A sustained firing pattern also emerged around the switch. Dual patch-clamp recordings from L4 neurons revealed that recurrent connections between L4 excitatory cells do not exist before and develop rapidly across the switch. In contrast, electrical coupling between these neurons waned around the switch. We suggest that maturation of electrophysiological features, particularly acquisition of a sustained firing pattern, and a transition from the immature electrical to mature chemical synaptic coupling between excitatory L4 neurons, contributes to the developmental switch in the cortical mode of function.

  4. Circuit Mechanisms Governing Local vs. Global Motion Processing in Mouse Visual Cortex

    DEFF Research Database (Denmark)

    Rasmussen, Rune; Yonehara, Keisuke

    2017-01-01

    components represented by component direction-selective (CDS) cells. However, how PDS and CDS cells develop their distinct response properties is still unresolved. The visual cortex of the mouse is an attractive model for experimentally solving this issue due to the large molecular and genetic toolbox...... literature on global motion processing based on works in primates and mice. Lastly, we propose what types of experiments could illuminate what circuit mechanisms are governing cortical global visual motion processing. We propose that PDS cells in mouse visual cortex appear as the perfect arena...

  5. Multiple blocks of intermittent and continuous theta-burst stimulation applied via transcranial magnetic stimulation differently affect sensory responses in rat barrel cortex.

    Science.gov (United States)

    Thimm, Andreas; Funke, Klaus

    2015-02-15

    Theta-burst stimulation (TBS) applied via transcranial magnetic stimulation is able to modulate human cortical excitability. Here we investigated in a rat model how two different forms of TBS, intermittent (iTBS) and continuous (cTBS), affect sensory responses in rat barrel cortex. We found that iTBS but less cTBS promoted late (>18 ms) sensory response components while not affecting the earliest response (8-18 ms). The effect increased with each of the five iTBS blocks applied. cTBS somewhat reduced the early response component after the first block but had a similar effect as iTBS after four to five blocks. We conclude that iTBS primarly modulates the activity of (inhibitory) cortical interneurons while cTBS may first reduce general neuronal excitability with a single block but reverse to iTBS-like effects with application of several blocks. Cortical sensory processing varies with cortical state and the balance of inhibition to excitation. Repetitive transcranial magnetic stimulation (rTMS) has been shown to modulate human cortical excitability. In a rat model, we recently showed that intermittent theta-burst stimulation (iTBS) applied to the corpus callosum, to activate primarily supragranular cortical pyramidal cells but fewer subcortical neurons, strongly reduced the cortical expression of parvalbumin (PV), indicating reduced activity of fast-spiking interneurons. Here, we used the well-studied rodent barrel cortex system to test how iTBS and continuous TBS (cTBS) modulate sensory responses evoked by either single or double stimuli applied to the principal (PW) and/or adjacent whisker (AW) in urethane-anaesthetized rats. Compared to sham stimulation, iTBS but not cTBS particularly enhanced late (>18 ms) response components of multi-unit spiking and local field potential responses in layer 4 but not the very early response (iTBS diminished the suppression of the second response evoked by paired PW or AW-PW stimulation at 20 ms intervals. The effects

  6. Inflammation and neuronal death in the motor cortex of the wobbler mouse, an ALS animal model

    DEFF Research Database (Denmark)

    Dahlke, Carolin; Saberi, Darius; Ott, Bastian

    2015-01-01

    microscopy, and transmission electron microscopy techniques, we analyze the proliferation behavior of microglial cells and astrocytes. We also investigate possible motor neuron death in the mouse motor cortex at different stages of the wobbler disease, which so far has not received much attention. Results...

  7. Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

    OpenAIRE

    Pilaz, Louis-Jan; Silver, Debra L.

    2014-01-01

    Although of short duration, mitosis is a complex and dynamic multi-step process fundamental for development of organs including the brain. In the developing cerebral cortex, abnormal mitosis of neural progenitors can cause defects in brain size and function. Hence, there is a critical need for tools to understand the mechanisms of neural progenitor mitosis. Cortical development in rodents is an outstanding model for studying this process. Neural progenitor mitosis is commonly examined in fixe...

  8. The coincident activation of lemniscal and paralemniscal inputs can drive synaptic plasticity in layer 2/3 pyramidal neurons of the mouse somatosensory cortex in vivo

    Directory of Open Access Journals (Sweden)

    Vassilis Kehayas

    2014-03-01

    Full Text Available Structural plasticity in the somatosensory cortex is maintained throughout life. In adult animals structural changes occur at the level of dendritic spines and axonal boutons in response to alterations in sensory experience. The causal relationship between synaptic activity and structural changes, however, is not clear. Hebbian-plasticity models predict that synapses will be stabilized at the nodes of neuronal networks that display high levels of coincident activity. Here, we aim at studying the effects of a targeted increase in coincident activity between segregated inputs on pyramidal cell synapses of the mouse somatosensory barrel cortex in vivo. Supragranular layers of the barrel cortex receive anatomically distinct inputs from two thalamic pathways: the ‘lemniscal’ pathway that originates in the ventral posteromedial (VPM nucleus and projects in a whisker-specific fashion to the barrel columns, and the ‘paralemniscal’ pathway that originates in the posteromedial (POm nucleus and projects to the cortex in a non-specific manner. Previous work from our lab shows that rhythmic (8Hz whisker stimulation-evoked LTP (RWS-LTP in layer (L 2/3 pyramidal cells relies on the combined activity of lemniscal and paralemniscal pathways. Here, we targeted ChR2 expression to POm neurons using AAV-mediated gene transfer in order to optically control the activity of those inputs. As a first step, we show that photostimulation of the POm nucleus induces NMDA-dependent, sub-threshold responses in L2/3 pyramidal cells similar to those that are required for the induction of RWS-LTP. In addition, simultaneous photostimulation of POm neurons together with whisker stimulation at low frequencies (1Hz can also elicit LTP, suggesting that coincident lemniscal and paralemniscal input can drive LTP induction. Next, we combined the ChR2-tdTomato expression in POm neurons with sparse AAV-mediated eGFP expression in L2/3 pyramidal cells in order to study the effects

  9. Experience-dependent spatial expectations in mouse visual cortex

    DEFF Research Database (Denmark)

    Fiser, Aris; Mahringer, David; Oyibo, Hassana K.

    2016-01-01

    In generative models of brain function, internal representations are used to generate predictions of sensory input, yet little is known about how internal models influence sensory processing. Here we show that, with experience in a virtual environment, the activity of neurons in layer 2/3 of mouse...

  10. Ketogenic diet alters dopaminergic activity in the mouse cortex.

    Science.gov (United States)

    Church, William H; Adams, Ryan E; Wyss, Livia S

    2014-06-13

    The present study was conducted to determine if the ketogenic diet altered basal levels of monoamine neurotransmitters in mice. The catecholamines dopamine (DA) and norephinephrine (NE) and the indolamine serotonin (5HT) were quantified postmortem in six different brain regions of adult mice fed a ketogenic diet for 3 weeks. The dopamine metabolites 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) and the serotonin metabolite 5-hydroxyindole acetic acid (5HIAA) were also measured. Tissue punches were collected bilaterally from the motor cortex, somatosensory cortex, nucleus accumbens, anterior caudate-putamen, posterior caudate-putamen and the midbrain. Dopaminergic activity, as measured by the dopamine metabolites to dopamine content ratio - ([DOPAC]+[HVA])/[DA] - was significantly increased in the motor and somatosensory cortex regions of mice fed the ketogenic diet when compared to those same areas in brains of mice fed a normal diet. These results indicate that the ketogenic diet alters the activity of the meso-cortical dopaminergic system, which may contribute to the diet's therapeutic effect in reducing epileptic seizure activity. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

  11. Prefrontal Cortex and Social Cognition in Mouse and Man

    Science.gov (United States)

    Bicks, Lucy K.; Koike, Hiroyuki; Akbarian, Schahram; Morishita, Hirofumi

    2015-01-01

    Social cognition is a complex process that requires the integration of a wide variety of behaviors, including salience, reward-seeking, motivation, knowledge of self and others, and flexibly adjusting behavior in social groups. Not surprisingly, social cognition represents a sensitive domain commonly disrupted in the pathology of a variety of psychiatric disorders including Autism Spectrum Disorder (ASD) and Schizophrenia (SCZ). Here, we discuss convergent research from animal models to human disease that implicates the prefrontal cortex (PFC) as a key regulator in social cognition, suggesting that disruptions in prefrontal microcircuitry play an essential role in the pathophysiology of psychiatric disorders with shared social deficits. We take a translational perspective of social cognition, and review three key behaviors that are essential to normal social processing in rodents and humans, including social motivation, social recognition, and dominance hierarchy. A shared prefrontal circuitry may underlie these behaviors. Social cognition deficits in animal models of neurodevelopmental disorders like ASD and SCZ have been linked to an altered balance of excitation and inhibition (E/I ratio) within the cortex generally, and PFC specifically. A clear picture of the mechanisms by which altered E/I ratio in the PFC might lead to disruptions of social cognition across a variety of behaviors is not well understood. Future studies should explore how disrupted developmental trajectory of prefrontal microcircuitry could lead to altered E/I balance and subsequent deficits in the social domain. PMID:26635701

  12. Live imaging of mitosis in the developing mouse embryonic cortex.

    Science.gov (United States)

    Pilaz, Louis-Jan; Silver, Debra L

    2014-06-04

    Although of short duration, mitosis is a complex and dynamic multi-step process fundamental for development of organs including the brain. In the developing cerebral cortex, abnormal mitosis of neural progenitors can cause defects in brain size and function. Hence, there is a critical need for tools to understand the mechanisms of neural progenitor mitosis. Cortical development in rodents is an outstanding model for studying this process. Neural progenitor mitosis is commonly examined in fixed brain sections. This protocol will describe in detail an approach for live imaging of mitosis in ex vivo embryonic brain slices. We will describe the critical steps for this procedure, which include: brain extraction, brain embedding, vibratome sectioning of brain slices, staining and culturing of slices, and time-lapse imaging. We will then demonstrate and describe in detail how to perform post-acquisition analysis of mitosis. We include representative results from this assay using the vital dye Syto11, transgenic mice (histone H2B-EGFP and centrin-EGFP), and in utero electroporation (mCherry-α-tubulin). We will discuss how this procedure can be best optimized and how it can be modified for study of genetic regulation of mitosis. Live imaging of mitosis in brain slices is a flexible approach to assess the impact of age, anatomy, and genetic perturbation in a controlled environment, and to generate a large amount of data with high temporal and spatial resolution. Hence this protocol will complement existing tools for analysis of neural progenitor mitosis.

  13. Cell proliferation, movement and differentiation during maintenance of the adult mouse adrenal cortex.

    Directory of Open Access Journals (Sweden)

    Su-Ping Chang

    Full Text Available Appropriate maintenance and regeneration of adult endocrine organs is important in both normal physiology and disease. We investigated cell proliferation, movement and differentiation in the adult mouse adrenal cortex, using different 5-bromo-2'-deoxyuridine (BrdU labelling regimens and immunostaining for phenotypic steroidogenic cell markers. Pulse-labelling showed that cell division was largely confined to the outer cortex, with most cells moving inwards towards the medulla at around 13-20 µm per day, though a distinct labelled cell population remained in the outer 10% of the cortex. Pulse-chase-labelling coupled with phenotypic immunostaining showed that, unlike cells in the inner cortex, most BrdU-positive outer cortical cells did not express steroidogenic markers, while co-staining for BrdU and Ki67 revealed that some outer cortical BrdU-positive cells were induced to proliferate following acute adrenocorticotropic hormone (ACTH treatment. Extended pulse-chase-labelling identified cells in the outer cortex which retained BrdU label for up to 18-23 weeks. Together, these observations are consistent with the location of both slow-cycling stem/progenitor and transiently amplifying cell populations in the outer cortex. Understanding the relationships between these distinct adrenocortical cell populations will be crucial to clarify mechanisms underpinning adrenocortical maintenance and long-term adaptation to pathophysiological states.

  14. Expression of aggrecan components in perineuronal nets in the mouse cerebral cortex

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    Hiroshi Ueno

    2018-06-01

    Full Text Available Specific regions of the cerebral cortex are highly plastic in an organism’s lifetime. It is thought that perineuronal nets (PNNs regulate plasticity, but labeling for Wisteria floribunda agglutinin (WFA, which is widely used to detect PNNs, is observed throughout the cortex. The aggrecan molecule—a PNN component—may regulate plasticity, and may also be involved in determining region-specific vulnerability to stress. To clarify cortical region-specific plasticity and vulnerability, we qualitatively analyzed aggrecan-positive and glycosylated aggrecan-positive PNNs in the mature mouse cerebral cortex. Our findings revealed the selective expression of both aggrecan-positive and glycosylated aggrecan-positive PNNs in the cortex. WFA-positive PNNs expressed aggrecan in a region-specific manner in the cortex. Furthermore, we observed variable distributions of PNNs containing WFA- and aggrecan-positive molecules. Together, our findings suggest that PNN components and their function differ depending on the cortical region, and that aggrecan molecules may be involved in determining region-specific plasticity and vulnerability in the cortex. Keywords: Aggrecan, Brain region-specific, Chondroitin sulfate proteoglycan, Extracellular matrix, Perineuronal nets, Plasticity

  15. Circuit Mechanisms Governing Local vs. Global Motion Processing in Mouse Visual Cortex

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    Rune Rasmussen

    2017-12-01

    Full Text Available A withstanding question in neuroscience is how neural circuits encode representations and perceptions of the external world. A particularly well-defined visual computation is the representation of global object motion by pattern direction-selective (PDS cells from convergence of motion of local components represented by component direction-selective (CDS cells. However, how PDS and CDS cells develop their distinct response properties is still unresolved. The visual cortex of the mouse is an attractive model for experimentally solving this issue due to the large molecular and genetic toolbox available. Although mouse visual cortex lacks the highly ordered orientation columns of primates, it is organized in functional sub-networks and contains striate- and extrastriate areas like its primate counterparts. In this Perspective article, we provide an overview of the experimental and theoretical literature on global motion processing based on works in primates and mice. Lastly, we propose what types of experiments could illuminate what circuit mechanisms are governing cortical global visual motion processing. We propose that PDS cells in mouse visual cortex appear as the perfect arena for delineating and solving how individual sensory features extracted by neural circuits in peripheral brain areas are integrated to build our rich cohesive sensory experiences.

  16. A Comparative Analysis of Perinatal Development of Barrel Cortex in Rat, Mouse and Guinea Pig Using Acetylcholinesterase Histochemistry

    OpenAIRE

    ŞENDEMİR, Erdoğan

    2014-01-01

    The role of acetylcholinesterase (AChE) in the developing neocortex was reexamined by comparing its expression in rats, mice and guinea pigs, following the protocol for acetylcholinesterase histochemistry (described in Sendemir et al., 1996) in order to determine the suitability of the breeding colony at UludaÛ University for use as an animal model. A total of 103 pups as well as two adult animals of each species were used. In the rat pups, acetylcholinesterase-rich patches were d...

  17. Morphology and distribution of chandelier cell axon terminals in the mouse cerebral cortex and claustroamygdaloid complex.

    Science.gov (United States)

    Inda, M C; DeFelipe, J; Muñoz, A

    2009-01-01

    Chandelier cells represent a unique type of cortical gamma-aminobutityric acidergic interneuron whose axon terminals (Ch-terminals) only form synapses with the axon initial segments of some pyramidal cells. Here, we have used immunocytochemistry for the high-affinity plasma membrane transporter GAT-1 and the calcium-binding protein parvalbumin to analyze the morphology and distribution of Ch-terminals in the mouse cerebral cortex and claustroamygdaloid complex. In general, 2 types of Ch-terminals were distinguished on the basis of their size and the density of the axonal boutons that made up the terminal. Simple Ch-terminals were made up of 1 or 2 rows of labeled boutons, each row consisting of only 3-5 boutons. In contrast, complex Ch-terminals were tight cylinder-like structures made up of multiple rows of boutons. Simple Ch-terminals were detected throughout the cerebral cortex and claustroamygdaloid complex, the complex type was only occasionally found in certain regions, whereas in others they were very abundant. These results indicate that there are substantial differences in the morphology and distribution of Ch-terminals between different areas and layers of the mouse cerebral cortex. Furthermore, we suggest that the distribution of complex Ch-terminals may be related to the developmental origin of the different brain regions analyzed.

  18. Multiple distinct subtypes of GABAergic neurons in mouse visual cortex identified by triple immunostaining

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    Yuri Gonchar

    2008-03-01

    Full Text Available The majority of cortical interneurons use GABA (gamma amino butyric acid as inhibitory neurotransmitter. GABAergic neurons are morphologically, connectionally, electrically and chemically heterogeneous. In rat cerebral cortex three distinct groups of GABAergic interneurons have been identifi ed by the expression of parvalbumin (PV, calretinin (CR and somatostatin (SOM. Recent studies in mouse cerebral cortex have revealed a different organization in which the CR and SOM populations are partially overlapping. Because CR and SOM neurons derive from different progenitors located in different embryonic structures, the coexpression of CR + SOM suggests that the chemical differentiation of interneurons is regulated postmitotically. Here, we have taken an important fi rst step towards understanding this process by triple immunostaining mouse visual cortex with a panel of antibodies, which has been used extensively for classifying developing interneurons. We have found at least 13 distinct groups of GABAergic neurons which include PV, CR, SOM, CCK (cholecystokinin, CR + SOM, CR + NPY (neuropeptide Y, CR + VIP (vasointestinal polypeptide, SOM + NPY, SOM + VIP, VIP + ChAT (choline acetyltransferase, CCK + NPY, CR + SOM + NPY and CR + SOM + VIP expressing cells. Triple immunostaining with PV, CR and SOM antibodies during postnatal development further showed that PV is never colocalized with CR and SOM. Importantly, expression of SOM and CR + SOM developed after the percentage of CR cells that do not express SOM has reached the mature level, suggesting that the chemical differentiation of SOM and CR + SOM neurons is a postnatal event, which may be controlled by transcriptional regulation.

  19. Automatic detection and quantitative analysis of cells in the mouse primary motor cortex

    Science.gov (United States)

    Meng, Yunlong; He, Yong; Wu, Jingpeng; Chen, Shangbin; Li, Anan; Gong, Hui

    2014-09-01

    Neuronal cells play very important role on metabolism regulation and mechanism control, so cell number is a fundamental determinant of brain function. Combined suitable cell-labeling approaches with recently proposed three-dimensional optical imaging techniques, whole mouse brain coronal sections can be acquired with 1-μm voxel resolution. We have developed a completely automatic pipeline to perform cell centroids detection, and provided three-dimensional quantitative information of cells in the primary motor cortex of C57BL/6 mouse. It involves four principal steps: i) preprocessing; ii) image binarization; iii) cell centroids extraction and contour segmentation; iv) laminar density estimation. Investigations on the presented method reveal promising detection accuracy in terms of recall and precision, with average recall rate 92.1% and average precision rate 86.2%. We also analyze laminar density distribution of cells from pial surface to corpus callosum from the output vectorizations of detected cell centroids in mouse primary motor cortex, and find significant cellular density distribution variations in different layers. This automatic cell centroids detection approach will be beneficial for fast cell-counting and accurate density estimation, as time-consuming and error-prone manual identification is avoided.

  20. BIASED AGONISM OF THREE DIFFERENT CANNABINOID RECEPTOR AGONISTS IN MOUSE BRAIN CORTEX

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    Rebeca Diez-Alarcia

    2016-11-01

    Full Text Available Cannabinoid receptors are able to couple to different families of G-proteins when activated by an agonist drug. It has been suggested that different intracellular responses may be activated depending on the ligand. The goal of the present study was to characterize the pattern of G protein subunit stimulation triggered by three different cannabinoid ligands, THC, WIN55212-2 and ACEA in mouse brain cortex.Stimulation of the [35S]GTPS binding coupled to specific immunoprecipitation with antibodies against different subtypes of G proteins (Gαi1, Gαi2, Gαi3, Gαo, Gαz, Gαs, Gαq/11, and Gα12/13, in the presence of Δ9-THC, WIN55212-2 and ACEA (submaximal concentration 10 µM was determined by Scintillation Proximity Assay (SPA technique in mouse cortex of wild type, CB1 knock-out, CB2 knock-out and CB1/CB2 double knock-out mice. Results show that, in mouse brain cortex, cannabinoid agonists are able to significantly stimulate not only the classical inhibitory Gαi/o subunits but also other G subunits like Gαz, Gαq/11, and Gα12/13. Moreover, the specific pattern of G protein subunit activation is different depending on the ligand. In conclusion, our results demonstrate that, in mice brain native tissue, different exogenous cannabinoid ligands are able to selectively activate different inhibitory and non-inhibitory Gα protein subtypes, through the activation of CB1 and/or CB2 receptors. Results of the present study may help to understand the specific molecular pathways involved in the pharmacological effects of cannabinoid-derived drugs.

  1. Intracellular responses to frequency modulated tones in the dorsal cortex of the mouse inferior colliculus

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    Ruediger eGeis

    2013-01-01

    Full Text Available Frequency modulations occur in many natural sounds, including vocalizations. The neuronal response to frequency modulated (FM stimuli has been studied extensively in different brain areas, with an emphasis on the auditory cortex and the central nucleus of the inferior colliculus. Here, we measured the responses to FM sweeps in whole-cell recordings from neurons in the dorsal cortex of the mouse inferior colliculus. Both up- and downward logarithmic FM sweeps were presented at two different speeds to both the ipsi- and the contralateral ear. Based on the number of action potentials that were fired, between 10-24% of cells were selective for rate or direction of the FM sweeps. A somewhat lower percentage of cells, 6-21%, showed selectivity based on EPSP size. To study the mechanisms underlying the generation of FM selectivity, we compared FM responses with responses to simple tones in the same cells. We found that if pairs of neurons responded in a similar way to simple tones, they generally also responded in a similar way to FM sweeps. Further evidence that FM selectivity can be generated within the dorsal cortex was obtained by reconstructing FM sweeps from the response to simple tones using three different models. In about half of the direction selective neurons the selectivity was generated by spectrally asymmetric synaptic inhibition. In addition, evidence for direction selectivity based on the timing of excitatory responses was also obtained in some cells. No clear evidence for the local generation of rate selectivity was obtained. We conclude that FM direction selectivity can be generated within the dorsal cortex of the mouse inferior colliculus by multiple mechanisms.

  2. Orientation selectivity of synaptic input to neurons in mouse and cat primary visual cortex.

    Science.gov (United States)

    Tan, Andrew Y Y; Brown, Brandon D; Scholl, Benjamin; Mohanty, Deepankar; Priebe, Nicholas J

    2011-08-24

    Primary visual cortex (V1) is the site at which orientation selectivity emerges in mammals: visual thalamus afferents to V1 respond equally to all stimulus orientations, whereas their target V1 neurons respond selectively to stimulus orientation. The emergence of orientation selectivity in V1 has long served as a model for investigating cortical computation. Recent evidence for orientation selectivity in mouse V1 opens cortical computation to dissection by genetic and imaging tools, but also raises two essential questions: (1) How does orientation selectivity in mouse V1 neurons compare with that in previously described species? (2) What is the synaptic basis for orientation selectivity in mouse V1? A comparison of orientation selectivity in mouse and in cat, where such measures have traditionally been made, reveals that orientation selectivity in mouse V1 is weaker than in cat V1, but that spike threshold plays a similar role in narrowing selectivity between membrane potential and spike rate. To uncover the synaptic basis for orientation selectivity, we made whole-cell recordings in vivo from mouse V1 neurons, comparing neuronal input selectivity-based on membrane potential, synaptic excitation, and synaptic inhibition-to output selectivity based on spiking. We found that a neuron's excitatory and inhibitory inputs are selective for the same stimulus orientations as is its membrane potential response, and that inhibitory selectivity is not broader than excitatory selectivity. Inhibition has different dynamics than excitation, adapting more rapidly. In neurons with temporally modulated responses, the timing of excitation and inhibition was different in mice and cats.

  3. Persistence of Functional Sensory Maps in the Absence of Cortical Layers in the Somsatosensory Cortex of Reeler Mice

    OpenAIRE

    Guy, Julien; Wagener, Robin J.; M?ck, Martin; Staiger, Jochen F.

    2014-01-01

    In rodents, layer IV of the primary somatosensory cortex contains the barrel field, where individual, large facial whiskers are represented as a dense cluster of cells. In the reeler mouse, a model of disturbed cortical development characterized by a loss of cortical lamination, the barrel field exists in a distorted manner. Little is known about the consequences of such a highly disturbed lamination on cortical function in this model. We used in vivo intrinsic signal optical imaging together...

  4. Organization of Estrogen-Associated Circuits in the Mouse Primary Auditory Cortex

    Directory of Open Access Journals (Sweden)

    Liisa A. Tremere

    2011-01-01

    Full Text Available Sex steroid hormones influence the perceptual processing of sensory signals in vertebrates. In particular, decades of research have shown that circulating levels of estrogen correlate with hearing function. The mechanisms and sites of action supporting this sensory-neuroendocrine modulation, however, remain unknown. Here we combined a molecular cloning strategy, fluorescence in-situ hybridization and unbiased quantification methods to show that estrogen-producing and -sensitive neurons heavily populate the adult mouse primary auditory cortex (AI. We also show that auditory experience in freely-behaving animals engages estrogen-producing and -sensitive neurons in AI. These estrogen-associated networks are greatly stable, and do not quantitatively change as a result of acute episodes of sensory experience. We further demonstrate the neurochemical identity of estrogen-producing and estrogen-sensitive neurons in AI and show that these cell populations are phenotypically distinct. Our findings provide the first direct demonstration that estrogen-associated circuits are highly prevalent and engaged by sensory experience in the mouse auditory cortex, and suggest that previous correlations between estrogen levels and hearing function may be related to brain-generated hormone production. Finally, our findings suggest that estrogenic modulation may be a central component of the operational framework of central auditory networks.

  5. Dynamics of dendritic spines in the mouse auditory cortex during memory formation and memory recall.

    Science.gov (United States)

    Moczulska, Kaja Ewa; Tinter-Thiede, Juliane; Peter, Manuel; Ushakova, Lyubov; Wernle, Tanja; Bathellier, Brice; Rumpel, Simon

    2013-11-05

    Long-lasting changes in synaptic connections induced by relevant experiences are believed to represent the physical correlate of memories. Here, we combined chronic in vivo two-photon imaging of dendritic spines with auditory-cued classical conditioning to test if the formation of a fear memory is associated with structural changes of synapses in the mouse auditory cortex. We find that paired conditioning and unpaired conditioning induce a transient increase in spine formation or spine elimination, respectively. A fraction of spines formed during paired conditioning persists and leaves a long-lasting trace in the network. Memory recall triggered by the reexposure of mice to the sound cue did not lead to changes in spine dynamics. Our findings provide a synaptic mechanism for plasticity in sound responses of auditory cortex neurons induced by auditory-cued fear conditioning; they also show that retrieval of an auditory fear memory does not lead to a recapitulation of structural plasticity in the auditory cortex as observed during initial memory consolidation.

  6. Barrels XXX meeting report: Barrels in Baltimore.

    Science.gov (United States)

    Shin, Hyeyoung; Bitzidou, Malamati; Palaguachi, Fernando; Brumberg, Joshua C

    2018-03-01

    The Barrels meeting annually brings together researchers focused on the rodent whisker to cortical barrel system prior to the Society for Neuroscience meeting. The 2017 meeting focused on the classification of cortical interneurons, the role interneurons have in shaping brain dynamics, and finally on the circuitry underlying oral sensations. The meeting highlighted the latest advancements in this rapidly advancing field.

  7. Effects of continuous low-dose prenatal irradiation on neuronal migration in mouse cerebral cortex

    International Nuclear Information System (INIS)

    Hyodo-Taguchi, Yasuko; Ishikawa, Yuji; Hirobe, Tomohisa; Fushiki, Shinji; Kinoshita, Chikako.

    1997-01-01

    We investigated the effects of continuous exposure to γ-rays during corticogenesis on the migration of neuronal cells in developing cerebral cortex. Pregnant mice were injected with 0.5 mg of bromodeoxyuridine (BrdU) on day 14 of gestation to label cells in the S phase. The mice were then exposed to 137 Cs γ-rays (dose rates of 0.1, 0.3, and 0.94 Gy/day) continuously for 3 days. Brains from 17-day-old embryos and from offspring at 3 and 8 weeks after birth were processed immunohistochemically to track the movements of BrdU-labeled cells. Comparative analyses of the distribution pattern of BrdU-labeled cells in the cerebral cortex revealed that the migration of neurons was delayed during the embryonic period in mice irradiated at 0.94 Gy/day, in 3-week-old mice, there was a significant difference in the distribution pattern of BrdU-labeled cells in the cerebral cortex between the mice irradiated prenatally and control, and in 8-week-old mice, there were no differences in the distribution pattern of BrdU-labeled cells between control and animals irradiated with 0.1 and 0.3 Gy/day. In contrast, in the animals irradiated with 0.94 Gy/day, the significant difference in the distribution pattern of the labeled cells relative to control was maintained. These results suggest that the migration of neuronal cells in mouse cerebral cortex is disturbed by continuous prenatal irradiation at low-dose and some modificational process occurred during the postnatal period. (author)

  8. A Corticocortical Circuit Directly Links Retrosplenial Cortex to M2 in the Mouse

    Science.gov (United States)

    Radulovic, Jelena

    2016-01-01

    Retrosplenial cortex (RSC) is a dorsomedial parietal area involved in a range of cognitive functions, including episodic memory, navigation, and spatial memory. Anatomically, the RSC receives inputs from dorsal hippocampal networks and in turn projects to medial neocortical areas. A particularly prominent projection extends rostrally to the posterior secondary motor cortex (M2), suggesting a functional corticocortical link from the RSC to M2 and thus a bridge between hippocampal and neocortical networks involved in mnemonic and sensorimotor aspects of navigation. We investigated the cellular connectivity in this RSC→M2 projection in the mouse using optogenetic photostimulation, retrograde labeling, and electrophysiology. Axons from RSC formed monosynaptic excitatory connections onto M2 pyramidal neurons across layers and projection classes, including corticocortical/intratelencephalic neurons (reciprocally and callosally projecting) in layers 2–6, pyramidal tract neurons (corticocollicular, corticopontine) in layer 5B, and, to a lesser extent, corticothalamic neurons in layer 6. In addition to these direct connections, disynaptic connections were made via posterior parietal cortex (RSC→PPC→M2) and anteromedial thalamus (RSC→AM→M2). In the reverse direction, axons from M2 monosynaptically excited M2-projecting corticocortical neurons in the RSC, especially in the superficial layers of the dysgranular region. These findings establish an excitatory RSC→M2 corticocortical circuit that engages diverse types of excitatory projection neurons in the downstream area, suggesting a basis for direct communication from dorsal hippocampal networks involved in spatial memory and navigation to neocortical networks involved in diverse aspects of sensorimotor integration and motor control. SIGNIFICANCE STATEMENT Corticocortical pathways interconnect cortical areas extensively, but the cellular connectivity in these pathways remains largely uncharacterized. Here, we

  9. Longitudinal Effects of Ketamine on Dendritic Architecture In Vivo in the Mouse Medial Frontal Cortex123

    Science.gov (United States)

    Phoumthipphavong, Victoria; Barthas, Florent; Hassett, Samantha

    2016-01-01

    Abstract A single subanesthetic dose of ketamine, an NMDA receptor antagonist, leads to fast-acting antidepressant effects. In rodent models, systemic ketamine is associated with higher dendritic spine density in the prefrontal cortex, reflecting structural remodeling that may underlie the behavioral changes. However, turnover of dendritic spines is a dynamic process in vivo, and the longitudinal effects of ketamine on structural plasticity remain unclear. The purpose of the current study is to use subcellular resolution optical imaging to determine the time course of dendritic alterations in vivo following systemic ketamine administration in mice. We used two-photon microscopy to visualize repeatedly the same set of dendritic branches in the mouse medial frontal cortex (MFC) before and after a single injection of ketamine or saline. Compared to controls, ketamine-injected mice had higher dendritic spine density in MFC for up to 2 weeks. This prolonged increase in spine density was driven by an elevated spine formation rate, and not by changes in the spine elimination rate. A fraction of the new spines following ketamine injection was persistent, which is indicative of functional synapses. In a few cases, we also observed retraction of distal apical tuft branches on the day immediately after ketamine administration. These results indicate that following systemic ketamine administration, certain dendritic inputs in MFC are removed immediately, while others are added gradually. These dynamic structural modifications are consistent with a model of ketamine action in which the net effect is a rebalancing of synaptic inputs received by frontal cortical neurons. PMID:27066532

  10. Differential Receptive Field Properties of Parvalbumin and Somatostatin Inhibitory Neurons in Mouse Auditory Cortex.

    Science.gov (United States)

    Li, Ling-Yun; Xiong, Xiaorui R; Ibrahim, Leena A; Yuan, Wei; Tao, Huizhong W; Zhang, Li I

    2015-07-01

    Cortical inhibitory circuits play important roles in shaping sensory processing. In auditory cortex, however, functional properties of genetically identified inhibitory neurons are poorly characterized. By two-photon imaging-guided recordings, we specifically targeted 2 major types of cortical inhibitory neuron, parvalbumin (PV) and somatostatin (SOM) expressing neurons, in superficial layers of mouse auditory cortex. We found that PV cells exhibited broader tonal receptive fields with lower intensity thresholds and stronger tone-evoked spike responses compared with SOM neurons. The latter exhibited similar frequency selectivity as excitatory neurons. The broader/weaker frequency tuning of PV neurons was attributed to a broader range of synaptic inputs and stronger subthreshold responses elicited, which resulted in a higher efficiency in the conversion of input to output. In addition, onsets of both the input and spike responses of SOM neurons were significantly delayed compared with PV and excitatory cells. Our results suggest that PV and SOM neurons engage in auditory cortical circuits in different manners: while PV neurons may provide broadly tuned feedforward inhibition for a rapid control of ascending inputs to excitatory neurons, the delayed and more selective inhibition from SOM neurons may provide a specific modulation of feedback inputs on their distal dendrites. © The Author 2014. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  11. Glycine receptors support excitatory neurotransmitter release in developing mouse visual cortex

    Science.gov (United States)

    Kunz, Portia A; Burette, Alain C; Weinberg, Richard J; Philpot, Benjamin D

    2012-01-01

    Glycine receptors (GlyRs) are found in most areas of the brain, and their dysfunction can cause severe neurological disorders. While traditionally thought of as inhibitory receptors, presynaptic-acting GlyRs (preGlyRs) can also facilitate glutamate release under certain circumstances, although the underlying molecular mechanisms are unknown. In the current study, we sought to better understand the role of GlyRs in the facilitation of excitatory neurotransmitter release in mouse visual cortex. Using whole-cell recordings, we found that preGlyRs facilitate glutamate release in developing, but not adult, visual cortex. The glycinergic enhancement of neurotransmitter release in early development depends on the high intracellular to extracellular Cl− gradient maintained by the Na+–K+–2Cl− cotransporter and requires Ca2+ entry through voltage-gated Ca2+ channels. The glycine transporter 1, localized to glial cells, regulates extracellular glycine concentration and the activation of these preGlyRs. Our findings demonstrate a developmentally regulated mechanism for controlling excitatory neurotransmitter release in the neocortex. PMID:22988142

  12. Thyroid Hormone Economy in the Perinatal Mouse Brain: Implications for Cerebral Cortex Development.

    Science.gov (United States)

    Bárez-López, Soledad; Obregon, Maria Jesus; Bernal, Juan; Guadaño-Ferraz, Ana

    2018-05-01

    Thyroid hormones (THs, T4 and the transcriptionally active hormone T3) play an essential role in neurodevelopment; however, the mechanisms underlying T3 brain delivery during mice fetal development are not well known. This work has explored the sources of brain T3 during mice fetal development using biochemical, anatomical, and molecular approaches. The findings revealed that during late gestation, a large amount of fetal brain T4 is of maternal origin. Also, in the developing mouse brain, fetal T3 content is regulated through the conversion of T4 into T3 by type-2 deiodinase (D2) activity, which is present from earlier prenatal stages. Additionally, D2 activity was found to be essential to mediate expression of T3-dependent genes in the cerebral cortex, and also necessary to generate the transient cerebral cortex hyperthyroidism present in mice lacking the TH transporter Monocarboxylate transporter 8. Notably, the gene encoding for D2 (Dio2) was mainly expressed at the blood-cerebrospinal fluid barrier (BCSFB). Overall, these data signify that T4 deiodinated by D2 may be the only source of T3 during neocortical development. We therefore propose that D2 activity at the BCSFB converts the T4 transported across the choroid plexus into T3, thus supplying the brain with active hormone to maintain TH homeostasis.

  13. Chronic ketamine reduces the peak frequency of gamma oscillations in mouse prefrontal cortex ex vivo

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    James M. McNally

    2013-09-01

    Full Text Available Abnormalities in EEG gamma band oscillations (GBO, 30-80 Hz serve as a prominent biomarker of schizophrenia (Sz, associated with positive, negative and cognitive symptoms. Chronic, subanesthetic administration of antagonists of N-methyl-D-aspartate receptors (NMDAR, such as ketamine, elicits behavioral effects and alterations in cortical interneurons similar to those observed in Sz. However, the chronic effects of ketamine on neocortical GBO are poorly understood. Thus, here we examine the effects of chronic (5 daily i.p. injections application of ketamine (5 and 30 mg/kg and the more specific NMDAR antagonist, MK-801 (0.02, 0.5, and 2 mg/kg, on neocortical GBO ex vivo. Oscillations were generated by focal application of the glutamate receptor agonist, kainate, in coronal brain slices containing the prelimbic cortex. This region constitutes the rodent analogue of the human dorsolateral prefrontal cortex, a brain region strongly implicated in Sz-pathophysiology. Here we report the novel finding that chronic ketamine elicits a reduction in the peak oscillatory frequency of kainate-elicited oscillations (from 47 to 40 Hz at 30 mg/kg. Moreover, the power of GBO in the 40-50 Hz band was reduced. These findings are reminiscent of both the reduced resonance frequency and power of cortical oscillations observed in Sz clinical studies. Surprisingly, MK-801 had no significant effect, suggesting care is needed when equating Sz-like behavioral effects elicited by different NMDAR antagonists to alterations in GBO activity. We conclude that chronic ketamine in the mouse mimics GBO abnormalities observed in Sz patients. Use of this ex vivo slice model may be useful in testing therapeutic compounds which rescue these GBO abnormalities.

  14. Synaptic Basis for Differential Orientation Selectivity between Complex and Simple Cells in Mouse Visual Cortex.

    Science.gov (United States)

    Li, Ya-tang; Liu, Bao-hua; Chou, Xiao-lin; Zhang, Li I; Tao, Huizhong W

    2015-08-05

    In the primary visual cortex (V1), orientation-selective neurons can be categorized into simple and complex cells primarily based on their receptive field (RF) structures. In mouse V1, although previous studies have examined the excitatory/inhibitory interplay underlying orientation selectivity (OS) of simple cells, the synaptic bases for that of complex cells have remained obscure. Here, by combining in vivo loose-patch and whole-cell recordings, we found that complex cells, identified by their overlapping on/off subfields, had significantly weaker OS than simple cells at both spiking and subthreshold membrane potential response levels. Voltage-clamp recordings further revealed that although excitatory inputs to complex and simple cells exhibited a similar degree of OS, inhibition in complex cells was more narrowly tuned than excitation, whereas in simple cells inhibition was more broadly tuned than excitation. The differential inhibitory tuning can primarily account for the difference in OS between complex and simple cells. Interestingly, the differential synaptic tuning correlated well with the spatial organization of synaptic input: the inhibitory visual RF in complex cells was more elongated in shape than its excitatory counterpart and also was more elongated than that in simple cells. Together, our results demonstrate that OS of complex and simple cells is differentially shaped by cortical inhibition based on its orientation tuning profile relative to excitation, which is contributed at least partially by the spatial organization of RFs of presynaptic inhibitory neurons. Simple and complex cells, two classes of principal neurons in the primary visual cortex (V1), are generally thought to be equally selective for orientation. In mouse V1, we report that complex cells, identified by their overlapping on/off subfields, has significantly weaker orientation selectivity (OS) than simple cells. This can be primarily attributed to the differential tuning selectivity

  15. Hi-C Chromatin Interaction Networks Predict Co-expression in the Mouse Cortex

    Science.gov (United States)

    Hulsman, Marc; Lelieveldt, Boudewijn P. F.; de Ridder, Jeroen; Reinders, Marcel

    2015-01-01

    The three dimensional conformation of the genome in the cell nucleus influences important biological processes such as gene expression regulation. Recent studies have shown a strong correlation between chromatin interactions and gene co-expression. However, predicting gene co-expression from frequent long-range chromatin interactions remains challenging. We address this by characterizing the topology of the cortical chromatin interaction network using scale-aware topological measures. We demonstrate that based on these characterizations it is possible to accurately predict spatial co-expression between genes in the mouse cortex. Consistent with previous findings, we find that the chromatin interaction profile of a gene-pair is a good predictor of their spatial co-expression. However, the accuracy of the prediction can be substantially improved when chromatin interactions are described using scale-aware topological measures of the multi-resolution chromatin interaction network. We conclude that, for co-expression prediction, it is necessary to take into account different levels of chromatin interactions ranging from direct interaction between genes (i.e. small-scale) to chromatin compartment interactions (i.e. large-scale). PMID:25965262

  16. Mapping functional connectivity in barrel-related columns reveals layer- and cell type-specific microcircuits.

    NARCIS (Netherlands)

    Schubert, D.; Kotter, R.; Staiger, J.F.

    2007-01-01

    Synaptic circuits bind together functional modules of the neocortex. We aim to clarify in a rodent model how intra- and transcolumnar microcircuits in the barrel cortex are laid out to segregate and also integrate sensory information. The primary somatosensory (barrel) cortex of rodents is the ideal

  17. Anthocyanins protect against LPS-induced oxidative stress-mediated neuroinflammation and neurodegeneration in the adult mouse cortex.

    Science.gov (United States)

    Khan, Muhammad Sohail; Ali, Tahir; Kim, Min Woo; Jo, Myeung Hoon; Jo, Min Gi; Badshah, Haroon; Kim, Myeong Ok

    2016-11-01

    Several studies provide evidence that reactive oxygen species (ROS) are key mediators of various neurological disorders. Anthocyanins are polyphenolic compounds and are well known for their anti-oxidant and neuroprotective effects. In this study, we investigated the neuroprotective effects of anthocyanins (extracted from black soybean) against lipopolysaccharide (LPS)-induced ROS-mediated neuroinflammation and neurodegeneration in the adult mouse cortex. Intraperitoneal injection of LPS (250 μg/kg) for 7 days triggers elevated ROS and oxidative stress, which induces neuroinflammation and neurodegeneration in the adult mouse cortex. Treatment with 24 mg/kg/day of anthocyanins for 14 days in LPS-injected mice (7 days before and 7 days co-treated with LPS) attenuated elevated ROS and oxidative stress compared to mice that received LPS-injection alone. The immunoblotting results showed that anthocyanins reduced the level of the oxidative stress kinase phospho-c-Jun N-terminal Kinase 1 (p-JNK). The immunoblotting and morphological results showed that anthocyanins treatment significantly reduced LPS-induced-ROS-mediated neuroinflammation through inhibition of various inflammatory mediators, such as IL-1β, TNF-α and the transcription factor NF- k B. Anthocyanins treatment also reduced activated astrocytes and microglia in the cortex of LPS-injected mice, as indicated by reductions in GFAP and Iba-1, respectively. Anthocyanins also prevent overexpression of various apoptotic markers, i.e., Bax, cytosolic cytochrome C, cleaved caspase-3 and PARP-1. Immunohistochemical fluoro-jade B (FJB) and Nissl staining indicated that anthocyanins prevent LPS-induced neurodegeneration in the mouse cortex. Our results suggest that dietary flavonoids, such as anthocyanins, have antioxidant and neuroprotective activities that could be beneficial to various neurological disorders. Copyright © 2016 Elsevier Ltd. All rights reserved.

  18. Molecular components and functions of the endocannabinoid system in mouse prefrontal cortex.

    Directory of Open Access Journals (Sweden)

    Mathieu Lafourcade

    2007-08-01

    Full Text Available Cannabinoids have deleterious effects on prefrontal cortex (PFC-mediated functions and multiple evidences link the endogenous cannabinoid (endocannabinoid system, cannabis use and schizophrenia, a disease in which PFC functions are altered. Nonetheless, the molecular composition and the physiological functions of the endocannabinoid system in the PFC are unknown.Here, using electron microscopy we found that key proteins involved in endocannabinoid signaling are expressed in layers v/vi of the mouse prelimbic area of the PFC: presynaptic cannabinoid CB1 receptors (CB1R faced postsynaptic mGluR5 while diacylglycerol lipase alpha (DGL-alpha, the enzyme generating the endocannabinoid 2-arachidonoyl-glycerol (2-AG was expressed in the same dendritic processes as mGluR5. Activation of presynaptic CB1R strongly inhibited evoked excitatory post-synaptic currents. Prolonged synaptic stimulation at 10Hz induced a profound long-term depression (LTD of layers V/VI excitatory inputs. The endocannabinoid -LTD was presynaptically expressed and depended on the activation of postsynaptic mGluR5, phospholipase C and a rise in postsynaptic Ca(2+ as predicted from the localization of the different components of the endocannabinoid system. Blocking the degradation of 2-AG (with URB 602 but not of anandamide (with URB 597 converted subthreshold tetanus to LTD-inducing ones. Moreover, inhibiting the synthesis of 2-AG with Tetrahydrolipstatin, blocked endocannabinoid-mediated LTD. All together, our data show that 2-AG mediates LTD at these synapses.Our data show that the endocannabinoid -retrograde signaling plays a prominent role in long-term synaptic plasticity at the excitatory synapses of the PFC. Alterations of endocannabinoid -mediated synaptic plasticity may participate to the etiology of PFC-related pathologies.

  19. Parvalbumin immunoreactivity in the auditory cortex of a mouse model of presbycusis.

    Science.gov (United States)

    Martin del Campo, H N; Measor, K R; Razak, K A

    2012-12-01

    Age-related hearing loss (presbycusis) affects ∼35% of humans older than sixty-five years. Symptoms of presbycusis include impaired discrimination of sounds with fast temporal features, such as those present in speech. Such symptoms likely arise because of central auditory system plasticity, but the underlying components are incompletely characterized. The rapid spiking inhibitory interneurons that co-express the calcium binding protein Parvalbumin (PV) are involved in shaping neural responses to fast spectrotemporal modulations. Here, we examined cortical PV expression in the C57bl/6 (C57) mouse, a strain commonly studied as a presbycusis model. We examined if PV expression showed auditory cortical field- and layer-specific susceptibilities with age. The percentage of PV-expressing cells relative to Nissl-stained cells was counted in the anterior auditory field (AAF) and primary auditory cortex (A1) in three age groups: young (1-2 months), middle-aged (6-8 months) and old (14-20 months). There were significant declines in the percentage of cells expressing PV at a detectable level in layers I-IV of both A1 and AAF in the old mice compared to young mice. In layers V-VI, there was an increase in the percentage of PV-expressing cells in the AAF of the old group. There were no changes in percentage of PV-expressing cells in layers V-VI of A1. These data suggest cortical layer(s)- and field-specific susceptibility of PV+ cells with presbycusis. The results are consistent with the hypothesis that a decline in inhibitory neurotransmission, particularly in the superficial cortical layers, occurs with presbycusis. Copyright © 2012 Elsevier B.V. All rights reserved.

  20. Assessment of the developmental totipotency of neural cells in the cerebral cortex of mouse embryo by nuclear transfer

    Science.gov (United States)

    Yamazaki, Yukiko; Makino, Hatsune; Hamaguchi-Hamada, Kayoko; Hamada, Shun; Sugino, Hidehiko; Kawase, Eihachiro; Miyata, Takaki; Ogawa, Masaharu; Yanagimachi, Ryuzo; Yagi, Takeshi

    2001-01-01

    When neural cells were collected from the entire cerebral cortex of developing mouse fetuses (15.5–17.5 days postcoitum) and their nuclei were transferred into enucleated oocytes, 5.5% of the reconstructed oocytes developed into normal offspring. This success rate was the highest among all previous mouse cloning experiments that used somatic cells. Forty-four percent of live embryos at 10.5 days postcoitum were morphologically normal when premature and early-postmitotic neural cells from the ventricular side of the cortex were used. In contrast, the majority (95%) of embryos were morphologically abnormal (including structural abnormalities in the neural tube) when postmitotic-differentiated neurons from the pial side of the cortex were used for cloning. Whereas 4.3% of embryos cloned with ventricular-side cells developed into healthy offspring, only 0.5% of those cloned with differentiated neurons in the pial side did so. These facts seem to suggest that the nuclei of neural cells in advanced stages of differentiation had lost their developmental totipotency. The underlying mechanism for this developmental limitation could be somatic DNA rearrangements in differentiating neural cells. PMID:11698647

  1. SCT Barrel Assembly Complete

    CERN Multimedia

    L. Batchelor

    As reported in the April 2005 issue of the ATLAS eNews, the first of the four Semiconductor Tracker (SCT) barrels, complete with modules and services, arrived safely at CERN in January of 2005. In the months since January, the other three completed barrels arrived as well, and integration of the four barrels into the entire barrel assembly commenced at CERN, in the SR1 building on the ATLAS experimental site, in July. Assembly was completed on schedule in September, with the addition of the innermost layer to the 4-barrel assembly. Work is now underway to seal the barrel thermal enclosure. This is necessary in order to enclose the silicon tracker in a nitrogen atmosphere and provide it with faraday-cage protection, and is a delicate and complicated task: 352 silicon module powertapes, 352 readout-fibre bundles, and over 400 Detector Control System sensors must be carefully sealed into the thermal enclosure bulkhead. The team is currently verifying the integrity of the low mass cooling system, which must be d...

  2. Mechanisms Underlying Serotonergic Excitation of Callosal Projection Neurons in the Mouse Medial Prefrontal Cortex

    Directory of Open Access Journals (Sweden)

    Emily K. Stephens

    2018-01-01

    Full Text Available Serotonin (5-HT selectively excites subpopulations of pyramidal neurons in the neocortex via activation of 5-HT2A (2A receptors coupled to Gq subtype G-protein alpha subunits. Gq-mediated excitatory responses have been attributed primarily to suppression of potassium conductances, including those mediated by KV7 potassium channels (i.e., the M-current, or activation of non-specific cation conductances that underlie calcium-dependent afterdepolarizations (ADPs. However, 2A-dependent excitation of cortical neurons has not been extensively studied, and no consensus exists regarding the underlying ionic effector(s involved. In layer 5 of the mouse medial prefrontal cortex, we tested potential mechanisms of serotonergic excitation in commissural/callosal (COM projection neurons, a subpopulation of pyramidal neurons that exhibits 2A-dependent excitation in response to 5-HT. In baseline conditions, 5-HT enhanced the rate of action potential generation in COM neurons experiencing suprathreshold somatic current injection. This serotonergic excitation was occluded by activation of muscarinic acetylcholine (ACh receptors, confirming that 5-HT acts via the same Gq-signaling cascades engaged by ACh. Like ACh, 5-HT promoted the generation of calcium-dependent ADPs following spike trains. However, calcium was not necessary for serotonergic excitation, as responses to 5-HT were enhanced (by >100%, rather than reduced, by chelation of intracellular calcium with 10 mM BAPTA. This suggests intracellular calcium negatively regulates additional ionic conductances gated by 2A receptors. Removal of extracellular calcium had no effect when intracellular calcium signaling was intact, but suppressed 5-HT response amplitudes, by about 50%, when BAPTA was included in patch pipettes. This suggests that 2A excitation involves activation of a non-specific cation conductance that is both calcium-sensitive and calcium-permeable. M-current suppression was found to be a third

  3. TRT Barrel milestones passed

    CERN Multimedia

    Ogren, H

    2004-01-01

    The barrel TRT detector passed three significant milestones this spring. The Barrel Support Structure (BSS) was completed and moved to the SR-1 building on February 24th. On March 12th the first module passed the quality assurance testing in Building 154 and was transported to the assembly site in the SR-1 building for barrel assembly. Then on April 21st the final production module that had been scanned at Hampton University was shipped to CERN. TRT Barrel Module Production The production of the full complement of barrel modules (96 plus 9 total spares) is now complete. This has been a five-year effort by Duke University, Hampton University, and Indiana University. Actual construction of the modules in the United States was completed in the first part of 2004. The production crews at each of the sites in the United States have now completed their missions. They are shown in the following pictures. Duke University: Production crew with the final completed module. Indiana University: Module producti...

  4. Barrelled locally convex spaces

    CERN Document Server

    Pérez Carreras, P

    1987-01-01

    This book is a systematic treatment of barrelled spaces, and of structures in which barrelledness conditions are significant. It is a fairly self-contained study of the structural theory of those spaces, concentrating on the basic phenomena in the theory, and presenting a variety of functional-analytic techniques.Beginning with some basic and important results in different branches of Analysis, the volume deals with Baire spaces, presents a variety of techniques, and gives the necessary definitions, exploring conditions on discs to ensure that they are absorbed by the barrels of the sp

  5. Barrel Module0 Autopsy

    CERN Document Server

    Cobal, M; Nessi, Marzio; Blanch, O; Zamora, Y

    1999-01-01

    Using the information from the Cs calibration runs, many of the problems affecting the response of the barrel Module0 prototype have been spotted out. These can be bad fibre-tile couplings, light losses from fibres bundling, broken fibres, not transparent tiles etc. After a visual inspection, most of these problems have been repaired.

  6. A crystal barrel

    CERN Multimedia

    2007-01-01

    The production of crystals for the barrel of the CMS electromagnetic calorimeter has been completed. This is an important milestone for the experiment, which received the last of its 62,960 crystals on 9 March. The members of the team responsible for the crystal acceptance testing at CERN display the last crystal for the CMS electromagnetic calorimeter barrel. From left to right: Igor Tarasov, Etiennette Auffray and Hervé Cornet.One of the six machines specially developed to measure 67 different parameters on each crystal. Igor Tarasov is seen inserting the last batch of crystals into the machine. The last of the 62,960 CMS barrel crystals arrived at CERN on 9 March. Once removed from its polystyrene protection, this delicate crystal, like thousands of its predecessors, will be inserted into the last of the 36 supermodules of the barrel electromagnetic calorimeter in a few days' time. This marks the end of an important chapter in an almost 15-year-long journey by the CMS crystals team, some of whose member...

  7. Double Dissociation of Spike Timing-Dependent Potentiation and Depression by Subunit-Preferring NMDA Receptor Antagonists in Mouse Barrel Cortex

    NARCIS (Netherlands)

    Banerjee, A.; Meredith, R.M.; Rodriguez-Moreno, A.; Mierau, S.B.; Auberson, Y.P.; Paulsen, O.

    2009-01-01

    Spike timing-dependent plasticity (STDP) is a strong candidate for an N-methyl-D-aspartate (NMDA) receptor-dependent form of synaptic plasticity that could underlie the development of receptive field properties in sensory neocortices. Whilst induction of timing-dependent long-term potentiation

  8. Oligodendrocyte- and Neuron-Specific Nogo-A Restrict Dendritic Branching and Spine Density in the Adult Mouse Motor Cortex.

    Science.gov (United States)

    Zemmar, Ajmal; Chen, Chia-Chien; Weinmann, Oliver; Kast, Brigitt; Vajda, Flora; Bozeman, James; Isaad, Noel; Zuo, Yi; Schwab, Martin E

    2018-06-01

    Nogo-A has been well described as a myelin-associated inhibitor of neurite outgrowth and functional neuroregeneration after central nervous system (CNS) injury. Recently, a new role of Nogo-A has been identified as a negative regulator of synaptic plasticity in the uninjured adult CNS. Nogo-A is present in neurons and oligodendrocytes. However, it is yet unclear which of these two pools regulate synaptic plasticity. To address this question we used newly generated mouse lines in which Nogo-A is specifically knocked out in (1) oligodendrocytes (oligoNogo-A KO) or (2) neurons (neuroNogo-A KO). We show that both oligodendrocyte- and neuron-specific Nogo-A KO mice have enhanced dendritic branching and spine densities in layer 2/3 cortical pyramidal neurons. These effects are compartmentalized: neuronal Nogo-A affects proximal dendrites whereas oligodendrocytic Nogo-A affects distal regions. Finally, we used two-photon laser scanning microscopy to measure the spine turnover rate of adult mouse motor cortex layer 5 cells and find that both Nogo-A KO mouse lines show enhanced spine remodeling after 4 days. Our results suggest relevant control functions of glial as well as neuronal Nogo-A for synaptic plasticity and open new possibilities for more selective and targeted plasticity enhancing strategies.

  9. Subtype-Specific Genes that Characterize Subpopulations of Callosal Projection Neurons in Mouse Identify Molecularly Homologous Populations in Macaque Cortex.

    Science.gov (United States)

    Fame, Ryann M; Dehay, Colette; Kennedy, Henry; Macklis, Jeffrey D

    2017-03-01

    Callosal projection neurons (CPN) interconnect the neocortical hemispheres via the corpus callosum and are implicated in associative integration of multimodal information. CPN have undergone differential evolutionary elaboration, leading to increased diversity of cortical neurons-and more extensive and varied connections in neocortical gray and white matter-in primates compared with rodents. In mouse, distinct sets of genes are enriched in discrete subpopulations of CPN, indicating the molecular diversity of rodent CPN. Elements of rodent CPN functional and organizational diversity might thus be present in the further elaborated primate cortex. We address the hypothesis that genes controlling mouse CPN subtype diversity might reflect molecular patterns shared among mammals that arose prior to the divergence of rodents and primates. We find that, while early expression of the examined CPN-enriched genes, and postmigratory expression of these CPN-enriched genes in deep layers are highly conserved (e.g., Ptn, Nnmt, Cited2, Dkk3), in contrast, the examined genes expressed by superficial layer CPN show more variable levels of conservation (e.g., EphA3, Chn2). These results suggest that there has been evolutionarily differential retraction and elaboration of superficial layer CPN subpopulations between mouse and macaque, with independent derivation of novel populations in primates. Together, these data inform future studies regarding CPN subpopulations that are unique to primates and rodents, and indicate putative evolutionary relationships. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  10. Characterization of primary and secondary cultures of astrocytes prepared from mouse cerebral cortex

    DEFF Research Database (Denmark)

    Skytt, Dorte Marie; Madsen, Karsten Kirkegaard; Pajecka, Kamilla

    2010-01-01

    Astrocyte cultures were prepared from cerebral cortex of new-born and 7-day-old mice and additionally, the cultures from new-born animals were passaged as secondary cultures. The cultures were characterized by immunostaining for the astrocyte markers glutamine synthetase (GS), glial fibrillary ac...... cerebral cortex of 7-day-old mice have metabolic and functional properties indistinguishable from those of classical astrocyte cultures prepared from neocortex of new-born animals. This provides flexibility with regard to preparation and use of these cultures for a variety of purposes....

  11. Cadherin-13 Deficiency Increases Dorsal Raphe 5-HT Neuron Density and Prefrontal Cortex Innervation in the Mouse Brain

    Directory of Open Access Journals (Sweden)

    Andrea Forero

    2017-09-01

    Full Text Available Background: During early prenatal stages of brain development, serotonin (5-HT-specific neurons migrate through somal translocation to form the raphe nuclei and subsequently begin to project to their target regions. The rostral cluster of cells, comprising the median and dorsal raphe (DR, innervates anterior regions of the brain, including the prefrontal cortex. Differential analysis of the mouse 5-HT system transcriptome identified enrichment of cell adhesion molecules in 5-HT neurons of the DR. One of these molecules, cadherin-13 (Cdh13 has been shown to play a role in cell migration, axon pathfinding, and synaptogenesis. This study aimed to investigate the contribution of Cdh13 to the development of the murine brain 5-HT system.Methods: For detection of Cdh13 and components of the 5-HT system at different embryonic developmental stages of the mouse brain, we employed immunofluorescence protocols and imaging techniques, including epifluorescence, confocal and structured illumination microscopy. The consequence of CDH13 loss-of-function mutations on brain 5-HT system development was explored in a mouse model of Cdh13 deficiency.Results: Our data show that in murine embryonic brain Cdh13 is strongly expressed on 5-HT specific neurons of the DR and in radial glial cells (RGCs, which are critically involved in regulation of neuronal migration. We observed that 5-HT neurons are intertwined with these RGCs, suggesting that these neurons undergo RGC-guided migration. Cdh13 is present at points of intersection between these two cell types. Compared to wildtype controls, Cdh13-deficient mice display increased cell densities in the DR at embryonic stages E13.5, E17.5, and adulthood, and higher serotonergic innervation of the prefrontal cortex at E17.5.Conclusion: Our findings provide evidence for a role of CDH13 in the development of the serotonergic system in early embryonic stages. Specifically, we indicate that Cdh13 deficiency affects the cell

  12. Response sensitivity of barrel neuron subpopulations to simulated thalamic input.

    Science.gov (United States)

    Pesavento, Michael J; Rittenhouse, Cynthia D; Pinto, David J

    2010-06-01

    Our goal is to examine the relationship between neuron- and network-level processing in the context of a well-studied cortical function, the processing of thalamic input by whisker-barrel circuits in rodent neocortex. Here we focus on neuron-level processing and investigate the responses of excitatory and inhibitory barrel neurons to simulated thalamic inputs applied using the dynamic clamp method in brain slices. Simulated inputs are modeled after real thalamic inputs recorded in vivo in response to brief whisker deflections. Our results suggest that inhibitory neurons require more input to reach firing threshold, but then fire earlier, with less variability, and respond to a broader range of inputs than do excitatory neurons. Differences in the responses of barrel neuron subtypes depend on their intrinsic membrane properties. Neurons with a low input resistance require more input to reach threshold but then fire earlier than neurons with a higher input resistance, regardless of the neuron's classification. Our results also suggest that the response properties of excitatory versus inhibitory barrel neurons are consistent with the response sensitivities of the ensemble barrel network. The short response latency of inhibitory neurons may serve to suppress ensemble barrel responses to asynchronous thalamic input. Correspondingly, whereas neurons acting as part of the barrel circuit in vivo are highly selective for temporally correlated thalamic input, excitatory barrel neurons acting alone in vitro are less so. These data suggest that network-level processing of thalamic input in barrel cortex depends on neuron-level processing of the same input by excitatory and inhibitory barrel neurons.

  13. The PANDA Barrel DIRC

    Science.gov (United States)

    Schwiening, J.; Ali, A.; Belias, A.; Dzhygadlo, R.; Gerhardt, A.; Götzen, K.; Kalicy, G.; Krebs, M.; Lehmann, D.; Nerling, F.; Patsyuk, M.; Peters, K.; Schepers, G.; Schmitt, L.; Schwarz, C.; Traxler, M.; Böhm, M.; Eyrich, W.; Lehmann, A.; Pfaffinger, M.; Uhlig, F.; Düren, M.; Etzelmüller, E.; Föhl, K.; Hayrapetyan, A.; Kreutzfeld, K.; Merle, O.; Rieke, J.; Schmidt, M.; Wasem, T.; Achenbach, P.; Cardinali, M.; Hoek, M.; Lauth, W.; Schlimme, S.; Sfienti, C.; Thiel, M.

    2018-03-01

    The PANDA experiment at the international accelerator Facility for Antiproton and Ion Research in Europe (FAIR) near GSI, Darmstadt, Germany will address fundamental questions of hadron physics. Excellent Particle Identification (PID) over a large range of solid angles and particle momenta will be essential to meet the objectives of the rich physics program. Charged PID for the barrel region of the PANDA target spectrometer will be provided by a DIRC (Detection of Internally Reflected Cherenkov light) detector. The Barrel DIRC will cover the polar angle range of 22o-140o and cleanly separate charged pions from kaons for momenta between 0.5 GeV/c and 3.5 GeV/c with a separation power of at least 3 standard deviations. The design is based on the successful BABAR DIRC and the SuperB FDIRC R&D with several important improvements to optimize the performance for PANDA, such as a focusing lens system, fast timing, a compact fused silica prism as expansion region, and lifetime-enhanced Microchannel-Plate PMTs for photon detection. This article describes the technical design of the PANDA Barrel DIRC and the result of the design validation using a "vertical slice" prototype in hadronic particle beams at the CERN PS.

  14. Publisher Correction: Single-cell analysis of experience-dependent transcriptomic states in the mouse visual cortex.

    Science.gov (United States)

    Hrvatin, Sinisa; Hochbaum, Daniel R; Nagy, M Aurel; Cicconet, Marcelo; Robertson, Keiramarie; Cheadle, Lucas; Zilionis, Rapolas; Ratner, Alex; Borges-Monroy, Rebeca; Klein, Allon M; Sabatini, Bernardo L; Greenberg, Michael E

    2018-05-11

    In the version of this article initially published, the x-axis labels in Fig. 3c read Vglut, Gad1/2, Aldh1l1 and Pecam1; they should have read Vglut + , Gad1/2 + , Aldh1l1 + and Pecam1 + . In Fig. 4, the range values were missing from the color scales; they are, from left to right, 4-15, 0-15, 4-15 and 0-15 in Fig. 4a and 4-15, 4-15 and 4-8 in Fig. 4h. In the third paragraph of the main text, the phrase reading "Previous approaches have analyzed a limited number of inhibitory cell types, thus masking the full diversity of excitatory populations" should have read "Previous approaches have analyzed a limited number of inhibitory cell types and masked the full diversity of excitatory populations." In the second paragraph of Results section "Diversity of experience-regulated ERGs," the phrase reading "thus suggesting considerable divergence within the gene expression program responding to early stimuli" should have read "thus suggesting considerable divergence within the early stimulus-responsive gene expression program." In the fourth paragraph of Results section "Excitatory neuronal LRGs," the sentence reading "The anatomical organization of these cell types into sublayers, coupled with divergent transcriptional responses to a sensory stimulus, suggested previously unappreciated functional subdivisions located within the laminae of the mouse visual cortex and resembling the cytoarchitecture in higher mammals" should have read "The anatomical organization of these cell types into sublayers, coupled with divergent transcriptional responses to a sensory stimulus, suggests previously unappreciated functional subdivisions located within the laminae of the mouse visual cortex, resembling the cytoarchitecture in higher mammals." In the last sentence of the Results, "sensory-responsive genes" should have read "sensory-stimulus-responsive genes." The errors have been corrected in the HTML and PDF versions of the article.

  15. HDAC2 expression in parvalbumin interneurons regulates synaptic plasticity in the mouse visual cortex

    Directory of Open Access Journals (Sweden)

    Alexi Nott

    2015-01-01

    Full Text Available An experience-dependent postnatal increase in GABAergic inhibition in the visual cortex is important for the closure of a critical period of enhanced synaptic plasticity. Although maturation of the subclass of parvalbumin (Pv–expressing GABAergic interneurons is known to contribute to critical period closure, the role of epigenetics on cortical inhibition and synaptic plasticity has not been explored. The transcription regulator, histone deacetylase 2 (HDAC2, has been shown to modulate synaptic plasticity and learning processes in hippocampal excitatory neurons. We found that genetic deletion of HDAC2 specifically from Pv interneurons reduces inhibitory input in the visual cortex of adult mice and coincides with enhanced long-term depression that is more typical of young mice. These findings show that HDAC2 loss in Pv interneurons leads to a delayed closure of the critical period in the visual cortex and supports the hypothesis that HDAC2 is a key negative regulator of synaptic plasticity in the adult brain.

  16. HDAC2 expression in parvalbumin interneurons regulates synaptic plasticity in the mouse visual cortex.

    Science.gov (United States)

    Nott, Alexi; Cho, Sukhee; Seo, Jinsoo; Tsai, Li-Huei

    2015-01-01

    An experience-dependent postnatal increase in GABAergic inhibition in the visual cortex is important for the closure of a critical period of enhanced synaptic plasticity. Although maturation of the subclass of Parvalbumin (Pv)-expressing GABAergic interneurons is known to contribute to critical period closure, the role of epigenetics on cortical inhibition and synaptic plasticity has not been explored. The transcription regulator, histone deacetylase 2 (HDAC2), has been shown to modulate synaptic plasticity and learning processes in hippocampal excitatory neurons. We found that genetic deletion of HDAC2 specifically from Pv-interneurons reduces inhibitory input in the visual cortex of adult mice, and coincides with enhanced long-term depression (LTD) that is more typical of young mice. These findings show that HDAC2 loss in Pv-interneurons leads to a delayed closure of the critical period in the visual cortex and supports the hypothesis that HDAC2 is a key negative regulator of synaptic plasticity in the adult brain.

  17. Voltage Imaging of Waking Mouse Cortex Reveals Emergence of Critical Neuronal Dynamics

    Science.gov (United States)

    Scott, Gregory; Fagerholm, Erik D.; Mutoh, Hiroki; Leech, Robert; Sharp, David J.; Shew, Woodrow L.

    2014-01-01

    Complex cognitive processes require neuronal activity to be coordinated across multiple scales, ranging from local microcircuits to cortex-wide networks. However, multiscale cortical dynamics are not well understood because few experimental approaches have provided sufficient support for hypotheses involving multiscale interactions. To address these limitations, we used, in experiments involving mice, genetically encoded voltage indicator imaging, which measures cortex-wide electrical activity at high spatiotemporal resolution. Here we show that, as mice recovered from anesthesia, scale-invariant spatiotemporal patterns of neuronal activity gradually emerge. We show for the first time that this scale-invariant activity spans four orders of magnitude in awake mice. In contrast, we found that the cortical dynamics of anesthetized mice were not scale invariant. Our results bridge empirical evidence from disparate scales and support theoretical predictions that the awake cortex operates in a dynamical regime known as criticality. The criticality hypothesis predicts that small-scale cortical dynamics are governed by the same principles as those governing larger-scale dynamics. Importantly, these scale-invariant principles also optimize certain aspects of information processing. Our results suggest that during the emergence from anesthesia, criticality arises as information processing demands increase. We expect that, as measurement tools advance toward larger scales and greater resolution, the multiscale framework offered by criticality will continue to provide quantitative predictions and insight on how neurons, microcircuits, and large-scale networks are dynamically coordinated in the brain. PMID:25505314

  18. Rp58 and p27kip1 coordinate cell cycle exit and neuronal migration within the embryonic mouse cerebral cortex.

    Science.gov (United States)

    Clément, Olivier; Hemming, Isabel Anne; Gladwyn-Ng, Ivan Enghian; Qu, Zhengdong; Li, Shan Shan; Piper, Michael; Heng, Julian Ik-Tsen

    2017-05-15

    During the development of the mammalian cerebral cortex, newborn postmitotic projection neurons are born from local neural stem cells and must undergo radial migration so as to position themselves appropriately to form functional neural circuits. The zinc finger transcriptional repressor Rp58 (also known as Znf238 or Zbtb18) is critical for coordinating corticogenesis, but its underlying molecular mechanism remains to be better characterised. Here, we demonstrate that the co-expression of Rp58 and the cyclin dependent kinase inhibitor (CDKI) p27 kip1 is important for E14.5-born cortical neurons to coordinate cell cycle exit and initiate their radial migration. Notably, we find that the impaired radial positioning of Rp58-deficient cortical neurons within the embryonic (E17.5) mouse cortex, as well as their multipolar to bipolar transition from the intermediate zone to the cortical plate can be restored by forced expression of p27 kip1 in concert with suppression of Rnd2, a downstream target gene of Rp58. Furthermore, the restorative effects of p27 kip1 and Rnd2 abrogation are reminiscent of suppressing RhoA signalling in Rp58-deficient cells. Our findings demonstrate functional interplay between a transcriptional regulator and a CDKI to mediate neuroprogenitor cell cycle exit, as well as to promote radial migration through a molecular mechanism consistent with suppression of RhoA signalling.

  19. Unusual patch-matrix organization in the retrosplenial cortex of the reeler mouse and Shaking rat Kawasaki.

    Science.gov (United States)

    Ichinohe, Noritaka; Knight, Adrian; Ogawa, Masaharu; Ohshima, Toshio; Mikoshiba, Katsuhiko; Yoshihara, Yoshihiro; Terashima, Toshio; Rockland, Kathleen S

    2008-05-01

    The rat granular retrosplenial cortex (GRS) is a simplified cortex, with distinct stratification and, in the uppermost layers, distinct modularity. Thalamic and cortical inputs are segregated by layers and in layer 1 colocalize, respectively, with apical dendritic bundles originating from neurons in layers 2 or 5. To further investigate this organization, we turned to reelin-deficient reeler mouse and Shaking rat Kawasaki. We found that the disrupted lamination, evident in Nissl stains in these rodents, is in fact a patch-matrix mosaic of segregated afferents and dendrites. Patches consist of thalamocortical connections, visualized by vesicular glutamate transporter 2 (VGluT2) or AChE. The surrounding matrix consists of corticocortical terminations, visualized by VGluT1 or zinc. Dendrites concentrate in the matrix or patches, depending on whether they are OCAM positive (matrix) or negative (patches). In wild-type rodents and, presumably, mutants, OCAM(+) structures originate from layer 5 neurons. By double labeling for dendrites (filled by Lucifer yellow in fixed slice) and OCAM immunofluorescence, we ascertained 2 populations in reeler: dendritic branches either preferred (putative layer 5 neurons) or avoided (putative supragranular neurons) the OCAM(+) matrix. We conclude that input-target relationships are largely preserved in the mutant GRS and that dendrite-dendrite interactions involving OCAM influence the formation of the mosaic configuration.

  20. LOCALIZATION OF NMDA AND AMPA RECEPTORS IN RAT BARREL FIELD

    NARCIS (Netherlands)

    JAARSMA, D; SEBENS, JB; KORF, J

    1991-01-01

    The aim of this study was to asses the distribution of N-methyl-D-aspartate (NMDA) and alpha-amino-3-hydroxy-S-methyl-4-isoxazole propionic acid (AMPA) receptors in the barrel field of rat primary somatosensory (SI) cortex using light-microscopic in vitro autoradiography. NMDA receptors were labeled

  1. Defects in the medial entorhinal cortex and dentate gyrus in the mouse model of Sanfilippo syndrome type B.

    Directory of Open Access Journals (Sweden)

    Kazuhiro Ohmi

    Full Text Available Sanfilippo syndrome type B (MPS IIIB is characterized by profound mental retardation in childhood, dementia and death in late adolescence; it is caused by deficiency of α-N-acetylglucosaminidase and resulting lysosomal storage of heparan sulfate. A mouse model, generated by homologous recombination of the Naglu gene, was used to study pathological changes in the brain. We found earlier that neurons in the medial entorhinal cortex (MEC and the dentate gyrus showed a number of secondary defects, including the presence of hyperphosphorylated tau (Ptau detected with antibodies raised against Ptau in Alzheimer disease brain. By further use of immunohistochemistry, we now show staining in neurons of the same area for beta amyloid, extending the resemblance to Alzheimer disease. Ptau inclusions in the dentate gyrus of MPS IIIB mice were reduced in number when the mice were administered LiCl, a specific inhibitor of Gsk3β. Additional proteins found elevated in MEC include proteins involved in autophagy and the heparan sulfate proteoglycans, glypicans 1 and 5, the latter closely related to the primary defect. The level of secondary accumulations was associated with elevation of glypican, as seen by comparing brains of mice at different ages or with different mucopolysaccharide storage diseases. The MEC of an MPS IIIA mouse had the same intense immunostaining for glypican 1 and other markers as MPS IIIB, while MEC of MPS I and MPS II mice had weak staining, and MEC of an MPS VI mouse had no staining at all for the same proteins. A considerable amount of glypican was found in MEC of MPS IIIB mice outside of lysosomes. We propose that it is the extralysosomal glypican that would be harmful to neurons, because its heparan sulfate branches could potentiate the formation of Ptau and beta amyloid aggregates, which would be toxic as well as difficult to degrade.

  2. Differential microRNA expression in the prefrontal cortex of mouse offspring induced by glyphosate exposure during pregnancy and lactation.

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    Ji, Hua; Xu, Linhao; Wang, Zheng; Fan, Xinli; Wu, Lihui

    2018-03-01

    Glyphosate is the active ingredient in numerous herbicide formulations. The role of glyphosate in neurotoxicity has been reported in human and animal models. However, the detailed mechanism of the role of glyphosate in neuronal development remains unknown. Recently, several studies have reported evidence linking neurodevelopmental disorders (NDDs) with gestational glyphosate exposure. The current group previously identified microRNAs (miRNAs) that are associated with the etiology of NDDs, but their expression levels in the developing brain following glyphosate exposure have not been characterized. In the present study, miRNA expression patterns were evaluated in the prefrontal cortex (PFC) of 28 postnatal day mouse offspring following glyphosate exposure during pregnancy and lactation. An miRNA microarray detected 55 upregulated and 19 downregulated miRNAs in the PFC of mouse offspring, and 20 selected deregulated miRNAs were further evaluated by quantitative polymerase chain reaction (PCR). A total of 11 targets of these selected deregulated miRNAs were analyzed using bioinformatics. Gene Ontology (GO) terms associated with the relevant miRNAs included neurogenesis (GO:0050769), neuron differentiation (GO:0030182) and brain development (GO:0007420). The genes Cdkn1a, Numbl, Notch1, Fosl1 and Lef1 are involved in the Wnt and Notch signaling pathways, which are closely associated with neural development. PCR arrays for the mouse Wnt and Notch signaling pathways were used to validate the effects of glyphosate on the expression pattern of genes involved in the Wnt and Notch pathways. Nr4a2 and Wnt7b were downregulated, while Dkk1, Dixdc1, Runx1, Shh, Lef-1 and Axin2 were upregulated in the PFC of mice offspring following glyphosate exposure during pregnancy and lactation. These results indicated abnormalities of the Wnt/β-catenin and Notch pathways. These findings may be of particular interest for understanding the mechanism of glyphosate-induced neurotoxicity, as

  3. Abnormal excitability and episodic low-frequency oscillations in the cerebral cortex of the tottering mouse.

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    Cramer, Samuel W; Popa, Laurentiu S; Carter, Russell E; Chen, Gang; Ebner, Timothy J

    2015-04-08

    The Ca(2+) channelopathies caused by mutations of the CACNA1A gene that encodes the pore-forming subunit of the human Cav2.1 (P/Q-type) voltage-gated Ca(2+) channel include episodic ataxia type 2 (EA2). Although, in EA2 the emphasis has been on cerebellar dysfunction, patients also exhibit episodic, nonmotoric abnormalities involving the cerebral cortex. This study demonstrates episodic, low-frequency oscillations (LFOs) throughout the cerebral cortex of tottering (tg/tg) mice, a widely used model of EA2. Ranging between 0.035 and 0.11 Hz, the LFOs in tg/tg mice can spontaneously develop very high power, referred to as a high-power state. The LFOs in tg/tg mice are mediated in part by neuronal activity as tetrodotoxin decreases the oscillations and cortical neuron discharge contain the same low frequencies. The high-power state involves compensatory mechanisms because acutely decreasing P/Q-type Ca(2+) channel function in either wild-type (WT) or tg/tg mice does not induce the high-power state. In contrast, blocking l-type Ca(2+) channels, known to be upregulated in tg/tg mice, reduces the high-power state. Intriguingly, basal excitatory glutamatergic neurotransmission constrains the high-power state because blocking ionotropic or metabotropic glutamate receptors results in high-power LFOs in tg/tg but not WT mice. The high-power LFOs are decreased markedly by acetazolamide and 4-aminopyridine, the primary treatments for EA2, suggesting disease relevance. Together, these results demonstrate that the high-power LFOs in the tg/tg cerebral cortex represent a highly abnormal excitability state that may underlie noncerebellar symptoms that characterize CACNA1A mutations. Copyright © 2015 the authors 0270-6474/15/355664-16$15.00/0.

  4. Histopathology of motor cortex in an experimental focal ischemic stroke in mouse model.

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    de Oliveira, Juçara Loli; Crispin, Pedro di Tárique Barreto; Duarte, Elisa Cristiana Winkelmann; Marloch, Gilberto Domingos; Gargioni, Rogério; Trentin, Andréa Gonçalves; Alvarez-Silva, Marcio

    2014-05-01

    Experimental ischemia results in cortical brain lesion followed by ischemic stroke. In this study, focal cerebral ischemia was induced in mice by occlusion of the middle cerebral artery. We studied cortical layers I, II/III, V and VI in the caudal forelimb area (CFA) and medial agranular cortex (AGm) from control and C57BL/6 mice induced with ischemic stroke. Based on our analysis of CFA and AGm motor cortex, significant differences were observed in the numbers of neurons, astrocytes and microglia in the superficial II/III and deep V cortical layers. Cellular changes were more prominent in layer V of the CFA with nuclear pyknosis, chromatin fragmentation, necrosis and degeneration, as well as, morphological evidence of apoptosis, mainly in neurons. As result, the CFA was more severely impaired than the AGm in this focal cerebral ischemic model, as evidenced by the proliferation of astrocytes, potentially resulting in neuroinflammation by microglia-like cells. Copyright © 2014 Elsevier B.V. All rights reserved.

  5. Circadian rhythm in adenosine A1 receptor of mouse cerebral cortex

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    Florio, C.; Rosati, A.M.; Traversa, U.; Vertua, R. (Univ. of Trieste (Italy))

    1991-01-01

    In order to investigate diurnal variation in adenosine A1 receptors binding parameters, Bmax and Kd values of specifically bound N6-cyclohexyl-({sup 3}H)adenosine were determined in the cerebral cortex of mice that had been housed under controlled light-dark cycles for 4 weeks. Significant differences were found for Bmax values measured at 3-hr intervals across a 24-h period, with low Bmax values during the light period and high Bmax values during the dark period. The amplitude between 03.00 and 18.00 hr was 33%. No substantial rhythm was found in the Kd values. It is suggested that the changes in the density of A1 receptors could reflect a physiologically-relevant mechanism by which adenosine exerts its modulatory role in the central nervous system.

  6. Circadian rhythm in adenosine A1 receptor of mouse cerebral cortex

    International Nuclear Information System (INIS)

    Florio, C.; Rosati, A.M.; Traversa, U.; Vertua, R.

    1991-01-01

    In order to investigate diurnal variation in adenosine A1 receptors binding parameters, Bmax and Kd values of specifically bound N6-cyclohexyl-[ 3 H]adenosine were determined in the cerebral cortex of mice that had been housed under controlled light-dark cycles for 4 weeks. Significant differences were found for Bmax values measured at 3-hr intervals across a 24-h period, with low Bmax values during the light period and high Bmax values during the dark period. The amplitude between 03.00 and 18.00 hr was 33%. No substantial rhythm was found in the Kd values. It is suggested that the changes in the density of A1 receptors could reflect a physiologically-relevant mechanism by which adenosine exerts its modulatory role in the central nervous system

  7. Neuropsychiatric Phenotypes Produced by GABA Reduction in Mouse Cortex and Hippocampus.

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    Kolata, Stefan M; Nakao, Kazuhito; Jeevakumar, Vivek; Farmer-Alroth, Emily L; Fujita, Yuko; Bartley, Aundrea F; Jiang, Sunny Zhihong; Rompala, Gregory R; Sorge, Robert E; Jimenez, Dennisse V; Martinowich, Keri; Mateo, Yolanda; Hashimoto, Kenji; Dobrunz, Lynn E; Nakazawa, Kazu

    2018-05-01

    Whereas cortical GAD67 reduction and subsequent GABA level decrease are consistently observed in schizophrenia and depression, it remains unclear how these GABAergic abnormalities contribute to specific symptoms. We modeled cortical GAD67 reduction in mice, in which the Gad1 gene is genetically ablated from ~50% of cortical and hippocampal interneurons. Mutant mice showed a reduction of tissue GABA in the hippocampus and cortex including mPFC, and exhibited a cluster of effort-based behavior deficits including decreased home-cage wheel running and increased immobility in both tail suspension and forced swim tests. Since saccharine preference, progressive ratio responding to food, and learned helplessness task were normal, such avolition-like behavior could not be explained by anhedonia or behavioral despair. In line with the prevailing view that dopamine in anterior cingulate cortex (ACC) plays a role in evaluating effort cost for engaging in actions, we found that tail-suspension triggered dopamine release in ACC of controls, which was severely attenuated in the mutant mice. Conversely, ACC dopamine release by progressive ratio responding to reward, during which animals were allowed to effortlessly perform the nose-poking, was not affected in mutants. These results suggest that cortical GABA reduction preferentially impairs the effort-based behavior which requires much effort with little benefit, through a deficit of ACC dopamine release triggered by high-effort cost behavior, but not by reward-seeking behavior. Collectively, a subset of negative symptoms with a reduced willingness to expend costly effort, often observed in patients with schizophrenia and depression, may be attributed to cortical GABA level reduction.

  8. Pharmacological or genetic orexin 1 receptor inhibition attenuates MK-801 induced glutamate release in mouse cortex

    Directory of Open Access Journals (Sweden)

    Leah eAluisio

    2014-05-01

    Full Text Available The orexin/hypocretin neuropeptides are produced by a cluster of neurons within the lateral posterior hypothalamus and participate in neuronal regulation by activating their receptors (OX1 and OX2 receptors. The orexin system projects widely through the brain and functions as an interface between multiple regulatory systems including wakefulness, energy balance, stress, reward and emotion. Recent studies have demonstrated that orexins and glutamate interact at the synaptic level and that orexins facilitate glutamate actions. We tested the hypothesis that orexins modulate glutamate signaling via OX1 receptors by monitoring levels of glutamate in frontal cortex of freely moving mice using enzyme coated biosensors under inhibited OX1 receptor conditions. MK-801, an NMDA receptor antagonist, was administered subcutaneously (0.178 mg/kg to indirectly disinhibit pyramidal neurons and therefore increase cortical glutamate release. In wild-type mice, pretreatment with the OX1 receptor antagonist GSK-1059865 (10 mg/kg S.C. which had no effect by itself, significantly attenuated the cortical glutamate release elicited by MK-801. OX1 receptor knockout mice had a blunted glutamate release response to MK-801 and exhibited about half of the glutamate release observed in wild-type mice in agreement with the data obtained with transient blockade of OX1 receptors. These results indicate that pharmacological (transient or genetic (permanent inhibition of the OX1 receptor similarly interfere with glutamatergic function in the cortex. Selectively targeting the OX1 receptor with an antagonist may normalize hyperglutamatergic states and thus may represent a novel therapeutic strategy for the treatment of various psychiatric disorders associated with hyperactive states.

  9. Visible light optical coherence microscopy imaging of the mouse cortex with femtoliter volume resolution

    Science.gov (United States)

    Merkle, Conrad W.; Chong, Shau Poh; Kho, Aaron M.; Zhu, Jun; Kholiqov, Oybek; Dubra, Alfredo; Srinivasan, Vivek J.

    2018-02-01

    Most flying-spot Optical Coherence Tomography (OCT) and Optical Coherence Microscopy (OCM) systems use a symmetric confocal geometry, where the detection path retraces the illumination path starting from and ending with the spatial mode of a single mode optical fiber. Here, we describe a visible light OCM instrument that breaks this symmetry to improve transverse resolution without sacrificing collection efficiency in scattering tissue. This was achieved by overfilling a 0.3 numerical aperture (NA) water immersion objective on the illumination path, while maintaining a conventional Gaussian mode detection path (1/e2 intensity diameter 0.82 Airy disks), enabling 1.1 μm full-width at half-maximum (FWHM) transverse resolution. At the same time, a 0.9 μm FWHM axial resolution in tissue, achieved by a broadband visible light source, enabled femtoliter volume resolution. We characterized this instrument according to paraxial coherent microscopy theory, and then used it to image the meningeal layers, intravascular red blood cell-free layer, and myelinated axons in the mouse neocortex in vivo through the thinned skull. Finally, by introducing a 0.8 NA water immersion objective, we improved the lateral resolution to 0.44 μm FWHM, which provided a volumetric resolution of 0.2 fL, revealing cell bodies in cortical layer I of the mouse brain with OCM for the first time.

  10. Proteomic profiling of brain cortex tissues in a Tau transgenic mouse model of Alzheimer's disease

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    Chang, Seong-Hun; Jung, In-Soo; Han, Gi-Yeon; Kim, Nam-Hee; Kim, Hyun-Jung [School of Life Sciences and Biotechnology, Korea University, Seoul 136-701 (Korea, Republic of); Kim, Chan-Wha, E-mail: cwkim@korea.ac.kr [School of Life Sciences and Biotechnology, Korea University, Seoul 136-701 (Korea, Republic of)

    2013-01-11

    Highlights: Black-Right-Pointing-Pointer A transgenic mouse model expressing NSE-htau23 was used. Black-Right-Pointing-Pointer 2D-gel electrophoresis to analyze the cortex proteins of transgenic mice was used. Black-Right-Pointing-Pointer Differentially expressed spots in different stages of AD were identified. Black-Right-Pointing-Pointer GSTP1 and CAII were downregulated with the progression of AD. Black-Right-Pointing-Pointer SCRN1 and ATP6VE1 were up regulated and down regulated differentially. -- Abstract: Alzheimer's disease (AD) involves regionalized neuronal death, synaptic loss, and an accumulation of intracellular neurofibrillary tangles and extracellular senile plaques. Although there have been numerous studies on tau proteins and AD in various stages of neurodegenerative disease pathology, the relationship between tau and AD is not yet fully understood. A transgenic mouse model expressing neuron-specific enolase (NSE)-controlled human wild-type tau (NSE-htau23), which displays some of the typical Alzheimer-associated pathological features, was used to analyze the brain proteome associated with tau tangle deposition. Two-dimensional electrophoresis was performed to compare the cortex proteins of transgenic mice (6- and 12-month-old) with those of control mice. Differentially expressed spots in different stages of AD were identified with ESI-Q-TOF (electrospray ionization quadruple time-of-flight) mass spectrometry and liquid chromatography/tandem mass spectrometry. Among the identified proteins, glutathione S-transferase P 1 (GSTP1) and carbonic anhydrase II (CAII) were down-regulated with the progression of AD, and secerin-1 (SCRN1) and V-type proton ATPase subunit E 1 (ATP6VE1) were up-regulated only in the early stages, and down-regulated in the later stages of AD. The proteins, which were further confirmed by RT-PCR at the mRNA level and with western blotting at the protein level, are expected to be good candidates as drug targets for AD. The

  11. Proteomic profiling of brain cortex tissues in a Tau transgenic mouse model of Alzheimer’s disease

    International Nuclear Information System (INIS)

    Chang, Seong-Hun; Jung, In-Soo; Han, Gi-Yeon; Kim, Nam-Hee; Kim, Hyun-Jung; Kim, Chan-Wha

    2013-01-01

    Highlights: ► A transgenic mouse model expressing NSE-htau23 was used. ► 2D-gel electrophoresis to analyze the cortex proteins of transgenic mice was used. ► Differentially expressed spots in different stages of AD were identified. ► GSTP1 and CAII were downregulated with the progression of AD. ► SCRN1 and ATP6VE1 were up regulated and down regulated differentially. -- Abstract: Alzheimer’s disease (AD) involves regionalized neuronal death, synaptic loss, and an accumulation of intracellular neurofibrillary tangles and extracellular senile plaques. Although there have been numerous studies on tau proteins and AD in various stages of neurodegenerative disease pathology, the relationship between tau and AD is not yet fully understood. A transgenic mouse model expressing neuron-specific enolase (NSE)-controlled human wild-type tau (NSE-htau23), which displays some of the typical Alzheimer-associated pathological features, was used to analyze the brain proteome associated with tau tangle deposition. Two-dimensional electrophoresis was performed to compare the cortex proteins of transgenic mice (6- and 12-month-old) with those of control mice. Differentially expressed spots in different stages of AD were identified with ESI-Q-TOF (electrospray ionization quadruple time-of-flight) mass spectrometry and liquid chromatography/tandem mass spectrometry. Among the identified proteins, glutathione S-transferase P 1 (GSTP1) and carbonic anhydrase II (CAII) were down-regulated with the progression of AD, and secerin-1 (SCRN1) and V-type proton ATPase subunit E 1 (ATP6VE1) were up-regulated only in the early stages, and down-regulated in the later stages of AD. The proteins, which were further confirmed by RT-PCR at the mRNA level and with western blotting at the protein level, are expected to be good candidates as drug targets for AD. The study of up- and down-regulation of proteins during the progression of AD helps to explain the mechanisms associated with neuronal

  12. Weapons barrel life cycle determination

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    Nebojša Pene Hristov

    2013-10-01

    Full Text Available This article describes the dynamic processes within the gun barrel during the firing process in exploitation. It generally defines the basic principles of constructing tube elements, and shows the distortion of the basic geometry of the tube interior due to wear as well as the impact it causes during exploitation. The article also defines basic empirical models as well as a model based on fracture mechanics for the calculation of a use-life of the barrel, and other elements essential for the safe use of the barrel as the basic weapon element. Erosion causes are analysed in order to control and reduce wear and prolong the lifetime of the gun barrel. It gives directions for the reparation of barrels with wasted resources. In conclusion, the most influential elements of tube wear are given as well as possible modifications of existing systems, primarily propellant charges, with a purpose of prolonging lifetime of gun barrels. The guidelines for a proper determination of the lifetime based on the barrel condition assessment are given as well. INTRODUCTION The barrel as the basic element of each weapon is described as well as the processes occurring during the firing that have impulsive character and are accompanied by large amounts of energy. The basic elements of barrel and itheir constructive characteristics are descibed. The relation between Internal ballistics, ie calculation of the propellant gas pressure in the firing process, and structural elements defined by the barrel material resistance is shown. In general, this part of the study explains the methodology of the gun barrel structural elements calculation, ie. barrel geometry, taking into account the degrees of safety in accordance with Military Standards.   TUBE WEAR AND DEFORMATIONS The weapon barrel gradually wears out during exploitation due to which it no longer satisfies the set requirements. It is considered that the barrel has experienced a lifetime when it fails to fulfill the

  13. Roles of molecular layer interneurons in sensory information processing in mouse cerebellar cortex Crus II in vivo.

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    Chun-Ping Chu

    Full Text Available Cerebellar cortical molecular layer interneurons (MLIs play essential roles in sensory information processing by the cerebellar cortex. However, recent experimental and modeling results are questioning traditional roles for molecular layer inhibition in the cerebellum.Synaptic responses of MLIs and Purkinje cells (PCs, evoked by air-puff stimulation of the ipsilateral whisker pad were recorded from cerebellar cortex Crus II in urethane-anesthetized ICR mice by in vivo whole-cell patch-clamp recording techniques. Under current-clamp (I = 0, air-puff stimuli were found to primarily produce inhibition in PCs. In MLIs, this stimulus evoked spike firing regardless of whether they made basket-type synaptic connections or not. However, MLIs not making basket-type synaptic connections had higher rates of background activity and also generated spontaneous spike-lets. Under voltage-clamp conditions, excitatory postsynaptic currents (EPSCs were recorded in MLIs, although the predominant response of recorded PCs was an inhibitory postsynaptic potential (IPSP. The latencies of EPSCs were similar for all MLIs, but the time course and amplitude of EPSCs varied with depth in the molecular layer. The highest amplitude, shortest duration EPSCs were recorded from MLIs deep in the molecular layer, which also made basket-type synaptic connections. Comparing MLI to PC responses, time to peak of PC IPSP was significantly slower than MLI recorded EPSCs. Blocking GABA(A receptors uncovered larger EPSCs in PCs whose time to peak, half-width and 10-90% rising time were also significantly slower than in MLIs. Biocytin labeling indicated that the MLIs (but not PCs are dye-coupled.These findings indicate that tactile face stimulation evokes rapid excitation in MLIs and inhibition occurring at later latencies in PCs in mouse cerebellar cortex Crus II. These results support previous suggestions that the lack of parallel fiber driven PC activity is due to the effect

  14. Cell-Specific Cholinergic Modulation of Excitability of Layer 5B Principal Neurons in Mouse Auditory Cortex

    Science.gov (United States)

    Joshi, Ankur; Kalappa, Bopanna I.; Anderson, Charles T.

    2016-01-01

    The neuromodulator acetylcholine (ACh) is crucial for several cognitive functions, such as perception, attention, and learning and memory. Whereas, in most cases, the cellular circuits or the specific neurons via which ACh exerts its cognitive effects remain unknown, it is known that auditory cortex (AC) neurons projecting from layer 5B (L5B) to the inferior colliculus, corticocollicular neurons, are required for cholinergic-mediated relearning of sound localization after occlusion of one ear. Therefore, elucidation of the effects of ACh on the excitability of corticocollicular neurons will bridge the cell-specific and cognitive properties of ACh. Because AC L5B contains another class of neurons that project to the contralateral cortex, corticocallosal neurons, to identify the cell-specific mechanisms that enable corticocollicular neurons to participate in sound localization relearning, we investigated the effects of ACh release on both L5B corticocallosal and corticocollicular neurons. Using in vitro electrophysiology and optogenetics in mouse brain slices, we found that ACh generated nicotinic ACh receptor (nAChR)-mediated depolarizing potentials and muscarinic ACh receptor (mAChR)-mediated hyperpolarizing potentials in AC L5B corticocallosal neurons. In corticocollicular neurons, ACh release also generated nAChR-mediated depolarizing potentials. However, in contrast to the mAChR-mediated hyperpolarizing potentials in corticocallosal neurons, ACh generated prolonged mAChR-mediated depolarizing potentials in corticocollicular neurons. These prolonged depolarizing potentials generated persistent firing in corticocollicular neurons, whereas corticocallosal neurons lacking mAChR-mediated depolarizing potentials did not show persistent firing. We propose that ACh-mediated persistent firing in corticocollicular neurons may represent a critical mechanism required for learning-induced plasticity in AC. SIGNIFICANCE STATEMENT Acetylcholine (ACh) is crucial for cognitive

  15. A Mouse Model of Visual Perceptual Learning Reveals Alterations in Neuronal Coding and Dendritic Spine Density in the Visual Cortex.

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    Wang, Yan; Wu, Wei; Zhang, Xian; Hu, Xu; Li, Yue; Lou, Shihao; Ma, Xiao; An, Xu; Liu, Hui; Peng, Jing; Ma, Danyi; Zhou, Yifeng; Yang, Yupeng

    2016-01-01

    Visual perceptual learning (VPL) can improve spatial vision in normally sighted and visually impaired individuals. Although previous studies of humans and large animals have explored the neural basis of VPL, elucidation of the underlying cellular and molecular mechanisms remains a challenge. Owing to the advantages of molecular genetic and optogenetic manipulations, the mouse is a promising model for providing a mechanistic understanding of VPL. Here, we thoroughly evaluated the effects and properties of VPL on spatial vision in C57BL/6J mice using a two-alternative, forced-choice visual water task. Briefly, the mice underwent prolonged training at near the individual threshold of contrast or spatial frequency (SF) for pattern discrimination or visual detection for 35 consecutive days. Following training, the contrast-threshold trained mice showed an 87% improvement in contrast sensitivity (CS) and a 55% gain in visual acuity (VA). Similarly, the SF-threshold trained mice exhibited comparable and long-lasting improvements in VA and significant gains in CS over a wide range of SFs. Furthermore, learning largely transferred across eyes and stimulus orientations. Interestingly, learning could transfer from a pattern discrimination task to a visual detection task, but not vice versa. We validated that this VPL fully restored VA in adult amblyopic mice and old mice. Taken together, these data indicate that mice, as a species, exhibit reliable VPL. Intrinsic signal optical imaging revealed that mice with perceptual training had higher cut-off SFs in primary visual cortex (V1) than those without perceptual training. Moreover, perceptual training induced an increase in the dendritic spine density in layer 2/3 pyramidal neurons of V1. These results indicated functional and structural alterations in V1 during VPL. Overall, our VPL mouse model will provide a platform for investigating the neurobiological basis of VPL.

  16. A mouse model of visual perceptual learning reveals alterations in neuronal coding and dendritic spine density in the visual cortex

    Directory of Open Access Journals (Sweden)

    Yan eWang

    2016-03-01

    Full Text Available Visual perceptual learning (VPL can improve spatial vision in normally sighted and visually impaired individuals. Although previous studies of humans and large animals have explored the neural basis of VPL, elucidation of the underlying cellular and molecular mechanisms remains a challenge. Owing to the advantages of molecular genetic and optogenetic manipulations, the mouse is a promising model for providing a mechanistic understanding of VPL. Here, we thoroughly evaluated the effects and properties of VPL on spatial vision in C57BL/6J mice using a two-alternative, forced-choice visual water task. Briefly, the mice underwent prolonged training at near the individual threshold of contrast or spatial frequency (SF for pattern discrimination or visual detection for 35 consecutive days. Following training, the contrast-threshold trained mice showed an 87% improvement in contrast sensitivity (CS and a 55% gain in visual acuity (VA. Similarly, the SF-threshold trained mice exhibited comparable and long-lasting improvements in VA and significant gains in CS over a wide range of SFs. Furthermore, learning largely transferred across eyes and stimulus orientations. Interestingly, learning could transfer from a pattern discrimination task to a visual detection task, but not vice versa. We validated that this VPL fully restored VA in adult amblyopic mice and old mice. Taken together, these data indicate that mice, as a species, exhibit reliable VPL. Intrinsic signal optical imaging revealed that mice with perceptual training had higher cut-off SFs in primary visual cortex (V1 than those without perceptual training. Moreover, perceptual training induced an increase in the dendritic spine density in layer 2/3 pyramidal neurons of V1. These results indicated functional and structural alterations in V1 during VPL. Overall, our VPL mouse model will provide a platform for investigating the neurobiological basis of VPL.

  17. The oil barrel price

    International Nuclear Information System (INIS)

    Blondy, J.; Papon, P.

    2009-01-01

    This paper proposes an overview and a prospective glance on the oil barrel price. It indicates the relevant indicators: Brent quotation, euro/dollar parity, economic activity indicators, world oil consumption distribution, crude oil production, refining capacity. It briefly presents the involved stake holders: crude oil producers, oil refiners, refined product dealers, and the OPEC. It discusses the major retrospective trends: evolution in relationship with geopolitical events and energy policies, strong correlation between oil demand and economic growth, prevalence of OPEC, growing importance of national oil companies. An emerging trend is noticed: growing role of emerging countries on the crude market. Some prospective issues are discussed: duration and intensity of economic recession, separation between economic growth and energy consumption, pace and ambition level of policies of struggle against climate change, exploitable resources, and geopolitical hazards. Four evolution hypotheses are discussed

  18. Temporal Sequence of Autolysis in the Cerebellar Cortex of the Mouse.

    Science.gov (United States)

    Finnie, J W; Blumbergs, P C; Manavis, J

    2016-05-01

    This study examined the temporal sequence of post-mortem changes in the cerebellar cortical granular and Purkinje cell layers of mice kept at a constant ambient temperature for up to 4 weeks. Nuclei of granule cell microneurons became pyknotic early after death, increasing progressively until, by 7 days, widespread nuclear lysis resulted in marked cellular depletion of the granular layer. Purkinje cells were relatively unaltered until about 96 h post mortem, at which time there was shrinkage and multivacuolation of the amphophilic cytoplasm, nuclear hyperchromasia and, sometimes, a perinuclear clear space. By 7 days, Purkinje cells had hypereosinophilic cytoplasm and frequent nuclear pyknosis. By 2 weeks after death, Purkinje cells showed homogenization, the cytoplasm being uniformly eosinophilic, progressing to a 'ghost-like' appearance in which the cytoplasm had pale eosinophilic staining with indistinct cell boundaries, and nuclei often absent. The results of this study could assist in differentiating post-mortem autolysis from ante-mortem lesions in the cerebellar cortex and determining the post-mortem interval. Moreover, this information could be useful when interpreting brain lesions in valuable mice found dead unexpectedly during the course of biomedical experiments. Crown Copyright © 2016. Published by Elsevier Ltd. All rights reserved.

  19. Population coding in mouse visual cortex: response reliability and dissociability of stimulus tuning and noise correlation

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    Jorrit S. Montijn

    2014-06-01

    Full Text Available The primary visual cortex is an excellent model system for investigating how neuronal populations encode information, because of well-documented relationships between stimulus characteristics and neuronal activation patterns. We used two-photon calcium imaging data to relate the performance of different methods for studying population coding (population vectors, template matching, and Bayesian decoding algorithms to their underlying assumptions. We show that the variability of neuronal responses may hamper the decoding of population activity, and that a normalization to correct for this variability may be of critical importance for correct decoding of population activity. Second, by comparing noise correlations and stimulus tuning we find that these properties have dissociated anatomical correlates, even though noise correlations have been previously hypothesized to reflect common synaptic input. We hypothesize that noise correlations arise from large non-specific increases in spiking activity acting on many weak synapses simultaneously, while neuronal stimulus response properties are dependent on more reliable connections. Finally, this paper provides practical guidelines for further research on population coding and shows that population coding cannot be approximated by a simple summation of inputs, but is heavily influenced by factors such as input reliability and noise correlation structure.

  20. Gamma-radiation produces abnormal Bergmann fibers and ectopic granule cells in mouse cerebellar cortex

    International Nuclear Information System (INIS)

    Inouye, Minoru; Hayasaka, Shizu; Funahashi, Atsushi; Yamamura, Hideki

    1992-01-01

    Morphological changes in Bergmann glial fibers in the developing cerebellar cortex produced by exposure to gamma-rays were investigated in association with ectopic granule cells. Six-day-old mice that had been exposed to 3 Gy of gamma-radiation were killed 6 hours after exposure or at 7 through 30 days of age. Their cerebella were examined histologically and immunohistochemically for glial fibrillary acidic protein in Bergmann fibers. Extensive cell death took place in the external granular layer (EGL) of the cerebellum from 6 through 24 hours after exposure. This led to the thinning of the EGL and a decrease in the number of migrating cells in the molecular layer. The number of Bergmann cells was not decreased, but the fibers in the molecular layer were distorted; whereas, in the control these fibers were straight and perpendicular to the pial surface. The EGL began to recover 2 days after exposure, and abnormally oriented migrating cells were seen. At 17 days of age, some cell clustering was observed in the molecular layer of the irradiated cerebellum. Distortion of the Bergmann fibers was marked in regions where ectopic granule cells appeared at 30 days of age. These findings suggest that the distortion of Bergmann fibers leads to the production of ectopic granule cells after exposure to gamma-radiation. (author)

  1. Diversity of layer 5 projection neurons in the mouse motor cortex

    Science.gov (United States)

    Oswald, Manfred J.; Tantirigama, Malinda L. S.; Sonntag, Ivo; Hughes, Stephanie M.; Empson, Ruth M.

    2013-01-01

    In the primary motor cortex (M1), layer 5 projection neurons signal directly to distant motor structures to drive movement. Despite their pivotal position and acknowledged diversity these neurons are traditionally separated into broad commissural and corticofugal types, and until now no attempt has been made at resolving the basis for their diversity. We therefore probed the electrophysiological and morphological properties of retrogradely labeled M1 corticospinal (CSp), corticothalamic (CTh), and commissural projecting corticostriatal (CStr) and corticocortical (CC) neurons. An unsupervised cluster analysis established at least four phenotypes with additional differences between lumbar and cervical projecting CSp neurons. Distinguishing parameters included the action potential (AP) waveform, firing behavior, the hyperpolarisation-activated sag potential, sublayer position, and soma and dendrite size. CTh neurons differed from CSp neurons in showing spike frequency acceleration and a greater sag potential. CStr neurons had the lowest AP amplitude and maximum rise rate of all neurons. Temperature influenced spike train behavior in corticofugal neurons. At 26°C CTh neurons fired bursts of APs more often than CSp neurons, but at 36°C both groups fired regular APs. Our findings provide reliable phenotypic fingerprints to identify distinct M1 projection neuron classes as a tool to understand their unique contributions to motor function. PMID:24137110

  2. Diversity of Layer 5 Projection Neurons in the Mouse Motor Cortex

    Directory of Open Access Journals (Sweden)

    Manfred J Oswald

    2013-10-01

    Full Text Available In the primary motor cortex (M1, layer 5 projection neurons signal directly to distant motor structures to drive movement. Despite their pivotal position and acknowledged diversity these neurons are traditionally separated into broad commissural and corticofugal types, and until now no attempt has been made at resolving the basis for their diversity. We therefore probed the electrophysiological and morphological properties of retrogradely labelled M1 corticospinal (CSp, corticothalamic (CTh, and commissural projecting corticostriatal (CStr and corticocortical (CC neurons. An unsupervised cluster analysis established at least four phenotypes with additional differences between lumbar and cervical projecting CSp neurons. Distinguishing parameters included the action potential (AP waveform, firing behaviour, the hyperpolarisation-activated sag potential, sublayer position, and soma and dendrite size. CTh neurons differed from CSp neurons in showing spike frequency acceleration and a greater sag potential. CStr neurons had the lowest AP amplitude and maximum rise rate of all neurons. Temperature influenced spike train behaviour in corticofugal neurons. At 26 ºC CTh neurons fired bursts of APs more often than CSp neurons, but at 36 ºC both groups fired regular APs. Our findings provide reliable phenotypic fingerprints to identify distinct M1 projection neuron classes as a tool to understand their unique contributions to motor function.

  3. Lack of functional specialization of neurons in the mouse primary visual cortex that have expressed calretinin

    Directory of Open Access Journals (Sweden)

    Daniela eCamillo

    2014-09-01

    Full Text Available Calretinin is a calcium-binding protein often used as a marker for a subset of inhibitory interneurons in the mammalian neocortex. We studied the labeled cells in offspring from a cross of a Cre-dependent reporter line with the CR-ires-Cre mice, which express Cre-recombinase in the same pattern as calretinin. We found that in the mature visual cortex, only a minority of the cells that have expressed calretinin and Cre-recombinase during their lifetime is GABAergic and only about 20% are immunoreactive for calretinin. The reason behind this is that calretinin is transiently expressed in many cortical pyramidal neurons during development. To determine whether neurons that express or have expressed calretinin share any distinct functional characteristics, we recorded their visual response properties using GCaMP6s calcium imaging. The average orientation selectivity, size tuning, and temporal and spatial frequency tuning of this group of cells, however, match the response profile of the general neuronal population, revealing the lack of functional specialization for the features studied.

  4. Sensory Deprivation during Early Postnatal Period Alters the Density of Interneurons in the Mouse Prefrontal Cortex

    Directory of Open Access Journals (Sweden)

    Hiroshi Ueno

    2015-01-01

    Full Text Available Early loss of one sensory system can cause improved function of other sensory systems. However, both the time course and neuronal mechanism of cross-modal plasticity remain elusive. Recent study using functional MRI in humans suggests a role of the prefrontal cortex (PFC in cross-modal plasticity. Since this phenomenon is assumed to be associated with altered GABAergic inhibition in the PFC, we have tested the hypothesis that early postnatal sensory deprivation causes the changes of inhibitory neuronal circuit in different regions of the PFC of the mice. We determined the effects of sensory deprivation from birth to postnatal day 28 (P28 or P58 on the density of parvalbumin (PV, calbindin (CB, and calretinin (CR neurons in the prelimbic, infralimbic, and dorsal anterior cingulate cortices. The density of PV and CB neurons was significantly increased in layer 5/6 (L5/6. Moreover, the density of CR neurons was higher in L2/3 in sensory deprived mice compared to intact mice. These changes were more prominent at P56 than at P28. These results suggest that long-term sensory deprivation causes the changes of intracortical inhibitory networks in the PFC and the changes of inhibitory networks in the PFC may contribute to cross-modal plasticity.

  5. The structure of pairwise correlation in mouse primary visual cortex reveals functional organization in the absence of an orientation map.

    Science.gov (United States)

    Denman, Daniel J; Contreras, Diego

    2014-10-01

    Neural responses to sensory stimuli are not independent. Pairwise correlation can reduce coding efficiency, occur independent of stimulus representation, or serve as an additional channel of information, depending on the timescale of correlation and the method of decoding. Any role for correlation depends on its magnitude and structure. In sensory areas with maps, like the orientation map in primary visual cortex (V1), correlation is strongly related to the underlying functional architecture, but it is unclear whether this correlation structure is an essential feature of the system or arises from the arrangement of cells in the map. We assessed the relationship between functional architecture and pairwise correlation by measuring both synchrony and correlated spike count variability in mouse V1, which lacks an orientation map. We observed significant pairwise synchrony, which was organized by distance and relative orientation preference between cells. We also observed nonzero correlated variability in both the anesthetized (0.16) and awake states (0.18). Our results indicate that the structure of pairwise correlation is maintained in the absence of an underlying anatomical organization and may be an organizing principle of the mammalian visual system preserved by nonrandom connectivity within local networks. © The Author 2013. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  6. Apoptotic mechanisms after repeated noise trauma in the mouse medial geniculate body and primary auditory cortex.

    Science.gov (United States)

    Fröhlich, Felix; Ernst, Arne; Strübing, Ira; Basta, Dietmar; Gröschel, Moritz

    2017-12-01

    A correlation between noise-induced apoptosis and cell loss has previously been shown after a single noise exposure in the cochlear nucleus, inferior colliculus, medial geniculate body (MGB) and primary auditory cortex (AI). However, repeated noise exposure is the most common situation in humans and a major risk factor for the induction of noise-induced hearing loss (NIHL). The present investigation measured cell death pathways using terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) in the dorsal, medial and ventral MGB (dMGB, mMGB and vMGB) and six layers of the AI (AI-1 to AI-6) in mice (NMRI strain) after a second noise exposure (double-exposure group). Therefore, a single noise exposure group has been investigated 7 (7-day-group-single) or 14 days (14-day-group-single) after noise exposure (3 h, 5-20 kHz, 115 dB SPL peak-to-peak). The double-exposure group received the same noise trauma for a second time 7 days after the initial exposure and was either TUNEL-stained immediately (7-day-group-double) or 1 week later (14-day-group-double) and data were compared to the corresponding single-trauma group as well as to an unexposed control group. It was shown that TUNEL increased immediately after the second noise exposure in AI-3 and stayed upregulated in the 14-day-group-double. A significant increase in TUNEL was also seen in the 14-day-group-double in vMGB, mMGB and AI-1. The present results show for the first time the influence of a repeated noise trauma on cell death mechanisms in thalamic and cortical structures and might contribute to the understanding of pathophysiological findings and psychoacoustic phenomena accompanying NIHL.

  7. Feature-Specific Organization of Feedback Pathways in Mouse Visual Cortex.

    Science.gov (United States)

    Huh, Carey Y L; Peach, John P; Bennett, Corbett; Vega, Roxana M; Hestrin, Shaul

    2018-01-08

    Higher and lower cortical areas in the visual hierarchy are reciprocally connected [1]. Although much is known about how feedforward pathways shape receptive field properties of visual neurons, relatively little is known about the role of feedback pathways in visual processing. Feedback pathways are thought to carry top-down signals, including information about context (e.g., figure-ground segmentation and surround suppression) [2-5], and feedback has been demonstrated to sharpen orientation tuning of neurons in the primary visual cortex (V1) [6, 7]. However, the response characteristics of feedback neurons themselves and how feedback shapes V1 neurons' tuning for other features, such as spatial frequency (SF), remain largely unknown. Here, using a retrograde virus, targeted electrophysiological recordings, and optogenetic manipulations, we show that putatively feedback neurons in layer 5 (hereafter "L5 feedback") in higher visual areas, AL (anterolateral area) and PM (posteromedial area), display distinct visual properties in awake head-fixed mice. AL L5 feedback neurons prefer significantly lower SF (mean: 0.04 cycles per degree [cpd]) compared to PM L5 feedback neurons (0.15 cpd). Importantly, silencing AL L5 feedback reduced visual responses of V1 neurons preferring low SF (mean change in firing rate: -8.0%), whereas silencing PM L5 feedback suppressed responses of high-SF-preferring V1 neurons (-20.4%). These findings suggest that feedback connections from higher visual areas convey distinctly tuned visual inputs to V1 that serve to boost V1 neurons' responses to SF. Such like-to-like functional organization may represent an important feature of feedback pathways in sensory systems and in the nervous system in general. Copyright © 2017 Elsevier Ltd. All rights reserved.

  8. Loss of Sleep Affects the Ultrastructure of Pyramidal Neurons in the Adolescent Mouse Frontal Cortex.

    Science.gov (United States)

    de Vivo, Luisa; Nelson, Aaron B; Bellesi, Michele; Noguti, Juliana; Tononi, Giulio; Cirelli, Chiara

    2016-04-01

    The adolescent brain may be uniquely affected by acute sleep deprivation (ASD) and chronic sleep restriction (CSR), but direct evidence is lacking. We used electron microscopy to examine how ASD and CSR affect pyramidal neurons in the frontal cortex of adolescent mice, focusing on mitochondria, endosomes, and lysosomes that together perform most basic cellular functions, from nutrient intake to prevention of cellular stress. Adolescent (1-mo-old) mice slept (S) or were sleep deprived (ASD, with novel objects and running wheels) during the first 6-8 h of the light period, chronically sleep restricted (CSR) for > 4 days (using novel objects, running wheels, social interaction, forced locomotion, caffeinated water), or allowed to recover sleep (RS) for ∼32 h after CSR. Ultrastructural analysis of 350 pyramidal neurons was performed (S = 82; ASD = 86; CSR = 103; RS = 79; 4 to 5 mice/group). Several ultrastructural parameters differed in S versus ASD, S versus CSR, CSR versus RS, and S versus RS, although the different methods used to enforce wake may have contributed to some of the differences between short and long sleep loss. Differences included larger cytoplasmic area occupied by mitochondria in CSR versus S, and higher number of secondary lysosomes in CSR versus S and RS. We also found that sleep loss may unmask interindividual differences not obvious during baseline sleep. Moreover, using a combination of 11 ultrastructural parameters, we could predict in up to 80% of cases whether sleep or wake occurred at the single cell level. Ultrastructural analysis may be a powerful tool to identify which cellular organelles, and thus which cellular functions, are most affected by sleep and sleep loss. © 2016 Associated Professional Sleep Societies, LLC.

  9. Secretin Modulates the Postnatal Development of Mouse Cerebellar Cortex Via PKA- and ERK-dependent Pathways

    Directory of Open Access Journals (Sweden)

    Lei Wang

    2017-11-01

    Full Text Available Postnatal development of the cerebellum is critical for its intact function such as motor coordination and has been implicated in the pathogenesis of psychiatric disorders. We previously reported that deprivation of secretin (SCT from cerebellar Purkinje neurons impaired motor coordination and motor learning function, while leaving the potential role of SCT in cerebellar development to be determined. SCT and its receptor (SCTR were constitutively expressed in the postnatal cerebellum in a temporal and cell-specific manner. Using a SCT knockout mouse model, we provided direct evidence showing altered developmental patterns of Purkinje cells (PCs and granular cells (GCs. SCT deprivation reduced the PC density, impaired the PC dendritic formation, induced accelerated GC migration and potentiated cerebellar apoptosis. Furthermore, our results indicated the involvement of protein kinase A (PKA and extracellular signal regulated kinase (ERK signaling pathways in SCT-mediated protective effects against neuronal apoptosis. Results of this study illustrated a novel function of SCT in the postnatal development of cerebellum, emphasizing the necessary role of SCT in cerebellar-related functions.

  10. Audiovisual Modulation in Mouse Primary Visual Cortex Depends on Cross-Modal Stimulus Configuration and Congruency.

    Science.gov (United States)

    Meijer, Guido T; Montijn, Jorrit S; Pennartz, Cyriel M A; Lansink, Carien S

    2017-09-06

    The sensory neocortex is a highly connected associative network that integrates information from multiple senses, even at the level of the primary sensory areas. Although a growing body of empirical evidence supports this view, the neural mechanisms of cross-modal integration in primary sensory areas, such as the primary visual cortex (V1), are still largely unknown. Using two-photon calcium imaging in awake mice, we show that the encoding of audiovisual stimuli in V1 neuronal populations is highly dependent on the features of the stimulus constituents. When the visual and auditory stimulus features were modulated at the same rate (i.e., temporally congruent), neurons responded with either an enhancement or suppression compared with unisensory visual stimuli, and their prevalence was balanced. Temporally incongruent tones or white-noise bursts included in audiovisual stimulus pairs resulted in predominant response suppression across the neuronal population. Visual contrast did not influence multisensory processing when the audiovisual stimulus pairs were congruent; however, when white-noise bursts were used, neurons generally showed response suppression when the visual stimulus contrast was high whereas this effect was absent when the visual contrast was low. Furthermore, a small fraction of V1 neurons, predominantly those located near the lateral border of V1, responded to sound alone. These results show that V1 is involved in the encoding of cross-modal interactions in a more versatile way than previously thought. SIGNIFICANCE STATEMENT The neural substrate of cross-modal integration is not limited to specialized cortical association areas but extends to primary sensory areas. Using two-photon imaging of large groups of neurons, we show that multisensory modulation of V1 populations is strongly determined by the individual and shared features of cross-modal stimulus constituents, such as contrast, frequency, congruency, and temporal structure. Congruent

  11. Core barrel inner tube lifter

    Energy Technology Data Exchange (ETDEWEB)

    Jeffers, J P

    1968-07-16

    A core drill with means for selectively lifting a core barrel inner tube consists of a lifting means connected to the core barrel inner tube assembly. It has a closable passage to permit drilling fluid normally to pass through it. The lifting means has a normally downward facing surface and a means to direct drilling fluid pressure against that surface so that on closure of the passage to fluid flow, the pressure of the drilling fluid is caused to act selectively on it. This causes the lifting means to rise and lift the core barrel. (7 claims)

  12. CMS Barrel Pixel Detector Overview

    CERN Document Server

    Kästli, H C; Erdmann, W; Gabathuler, K; Hörmann, C; Horisberger, Roland Paul; König, S; Kotlinski, D; Meier, B; Robmann, P; Rohe, T; Streuli, S

    2007-01-01

    The pixel detector is the innermost tracking device of the CMS experiment at the LHC. It is built from two independent sub devices, the pixel barrel and the end disks. The barrel consists of three concentric layers around the beam pipe with mean radii of 4.4, 7.3 and 10.2 cm. There are two end disks on each side of the interaction point at 34.5 cm and 46.5 cm. This article gives an overview of the pixel barrel detector, its mechanical support structure, electronics components, services and its expected performance.

  13. Increased transient Na+ conductance and action potential output in layer 2/3 prefrontal cortex neurons of the fmr1-/y mouse.

    Science.gov (United States)

    Routh, Brandy N; Rathour, Rahul K; Baumgardner, Michael E; Kalmbach, Brian E; Johnston, Daniel; Brager, Darrin H

    2017-07-01

    Layer 2/3 neurons of the prefrontal cortex display higher gain of somatic excitability, responding with a higher number of action potentials for a given stimulus, in fmr1 -/y mice. In fmr1 -/y L2/3 neurons, action potentials are taller, faster and narrower. Outside-out patch clamp recordings revealed that the maximum Na + conductance density is higher in fmr1 -/y L2/3 neurons. Measurements of three biophysically distinct K + currents revealed a depolarizing shift in the activation of a rapidly inactivating (A-type) K + conductance. Realistic neuronal simulations of the biophysical observations recapitulated the elevated action potential and repetitive firing phenotype. Fragile X syndrome is the most common form of inherited mental impairment and autism. The prefrontal cortex is responsible for higher order cognitive processing, and prefrontal dysfunction is believed to underlie many of the cognitive and behavioural phenotypes associated with fragile X syndrome. We recently demonstrated that somatic and dendritic excitability of layer (L) 5 pyramidal neurons in the prefrontal cortex of the fmr1 -/y mouse is significantly altered due to changes in several voltage-gated ion channels. In addition to L5 pyramidal neurons, L2/3 pyramidal neurons play an important role in prefrontal circuitry, integrating inputs from both lower brain regions and the contralateral cortex. Using whole-cell current clamp recording, we found that L2/3 pyramidal neurons in prefrontal cortex of fmr1 -/y mouse fired more action potentials for a given stimulus compared with wild-type neurons. In addition, action potentials in fmr1 -/y neurons were significantly larger, faster and narrower. Voltage clamp of outside-out patches from L2/3 neurons revealed that the transient Na + current was significantly larger in fmr1 -/y neurons. Furthermore, the activation curve of somatic A-type K + current was depolarized. Realistic conductance-based simulations revealed that these biophysical changes in Na

  14. Time course of cell death due to acoustic overstimulation in the mouse medial geniculate body and primary auditory cortex

    Directory of Open Access Journals (Sweden)

    Felix Frohlich

    2017-01-01

    Full Text Available It has previously been shown that acoustic overstimulation induces cell death and extensive cell loss in key structures of the central auditory pathway. A correlation between noise-induced apoptosis and cell loss was hypothesized for the cochlear nucleus and colliculus inferior. To determine the role of cell death in noise-induced cell loss in thalamic and cortical structures, the present mouse study (NMRI strain describes the time course following noise exposure of cell death mechanisms for the ventral medial geniculate body (vMGB, medial MGB (mMGB, and dorsal MGB (dMGB and the six histological layers of the primary auditory cortex (AI 1–6. Therefore, a terminal deoxynucleotidyl transferase dioxyuridine triphosphate nick-end labeling assay (TUNEL was performed in these structures 24 h, 7 days, and 14 days after noise exposure (3 h, 115 dB sound pressure level, 5–20 kHz, as well as in unexposed controls. In the dMGB, TUNEL was statistically significant elevated 24 h postexposure. AI-1 showed a decrease in TUNEL after 14 days. There was no statistically significant difference between groups for the other brain areas investigated. dMGB’s widespread connection within the central auditory pathway and its nontonotopical organization might explain its prominent increase in TUNEL compared to the other MGB subdivisions and the AI. It is assumed that the onset and peak of noise-induced cell death is delayed in higher areas of the central auditory pathway and takes place between 24 h and 7 days postexposure in thalamic and cortical structures.

  15. Laminar microvascular transit time distribution in the mouse somatosensory cortex revealed by Dynamic Contrast Optical Coherence Tomography.

    Science.gov (United States)

    Merkle, Conrad W; Srinivasan, Vivek J

    2016-01-15

    The transit time distribution of blood through the cerebral microvasculature both constrains oxygen delivery and governs the kinetics of neuroimaging signals such as blood-oxygen-level-dependent functional Magnetic Resonance Imaging (BOLD fMRI). However, in spite of its importance, capillary transit time distribution has been challenging to quantify comprehensively and efficiently at the microscopic level. Here, we introduce a method, called Dynamic Contrast Optical Coherence Tomography (DyC-OCT), based on dynamic cross-sectional OCT imaging of an intravascular tracer as it passes through the field-of-view. Quantitative transit time metrics are derived from temporal analysis of the dynamic scattering signal, closely related to tracer concentration. Since DyC-OCT does not require calibration of the optical focus, quantitative accuracy is achieved even deep in highly scattering brain tissue where the focal spot degrades. After direct validation of DyC-OCT against dilution curves measured using a fluorescent plasma label in surface pial vessels, we used DyC-OCT to investigate the transit time distribution in microvasculature across the entire depth of the mouse somatosensory cortex. Laminar trends were identified, with earlier transit times and less heterogeneity in the middle cortical layers. The early transit times in the middle cortical layers may explain, at least in part, the early BOLD fMRI onset times observed in these layers. The layer-dependencies in heterogeneity may help explain how a single vascular supply manages to deliver oxygen to individual cortical layers with diverse metabolic needs. Copyright © 2015 Elsevier Inc. All rights reserved.

  16. Core barrel plug

    International Nuclear Information System (INIS)

    Tolino, R.W.; Hopkins, R.J.; Congleton, R.L.; Popalis, C.H.

    1986-01-01

    A plug is described for preventing flow through a port in a core barrel of a pressurized water nuclear reactor which consists of: a substantially cylindrical body formed with a cylindrical portion and a flange and defining a tapered leading open end with the other end being closed by an end plug attached to the flange, the body defining a bore therein extending from the open end to the end plug with the bore having a smaller diameter near the open end than near the end plug, the cylindrical portion having a lip near the open end and being formed with longitudinal slots extending from the open end toward the flange and extending entirely through the thickness of the cylindrical portion, the cylindrical portion having a circumferential first groove on the outer surface thereof located near the forwardmost portion of the cylindrical portion but not in the section of the cylindrical portion that has the slots therein, and a plurality of circumferential second grooves on the outer surface thereof located in the section of the cylindrical portion that has the slots therein, the first and second grooves establishing a seal between the cylindrical portion and the inside surface of the port when the cylindrical portion is expanded, and the flange and the end plug having a passageway defined therein for introducing a fluid into the body; a metal ring disposed in each of the second grooves; a mandrel slidably disposed and captured in the body and capable of being moved toward the open end of the body when the fluid is introduced through the passageway, thereby causing the cylindrical portion to be expanded into contact with the inside surface of the port; and a locking mechanism disposed in the end plug for preventing inadvertent movement of the mandrel

  17. ID Barrel installed in cryostat

    CERN Multimedia

    Apsimon, R.; Romaniouk, A.

    Wednesday 23rd August was a memorable day for the Inner Detector community as they witnessed the transport and installation of the central part of the inner detector (ID-barrel) into the ATLAS detector. Many members of the collaboration gathered to witness this moment at Point 1. After years of design, construction and commissioning, the outer two detectors (TRT and SCT) of the ID barrel were moved from the SR1 cleanroom to the ATLAS cavern. The barrel was moved across the car park from building 2175 to SX1. Although only a journey of about 100 metres, this required weeks of planning and some degree of luck as far as the weather was concerned. Accelerometers were fitted to the barrel to provide real-time monitoring and no values greater than 0.1 g were recorded, fully satisfying the transport specification for this extremely precise and fragile detector. Muriel, despite her fear of heights, bravely volunteered to keep a close eye on the detector. Swapping cranes to cross the entire parking lot, while Mur...

  18. Work on a ATLAS tile calorimeter Barrel

    CERN Multimedia

    Laurent Guiraud

    2000-01-01

    The Tile Calorimeter is designed as one barrel and two extended barrel hadron parts. The calorimeter consists of a cylindrical structure with inner and outer radius of 2280 and 4230 mm respectively. The barrel part is 5640 mm in length along the beam axis, while each of the extended barrel cylinders is 2910 mm long. Each detector cylinder is built of 64 independent wedges along the azimuthal direction. Between the barrel and the extended barrels there is a gap of about 600 mm, which is needed for the Inner Detector and the Liquid Argon cables, electronics and services. The barrel covers the region -1.0barrels cover the region 0.8<|h|<1.7.

  19. Social isolation stress and chronic glutathione deficiency have a common effect on the glutamine-to-glutamate ratio and myo-inositol concentration in the mouse frontal cortex.

    Science.gov (United States)

    Corcoba, Alberto; Gruetter, Rolf; Do, Kim Q; Duarte, João M N

    2017-09-01

    Environmental stress can interact with genetic predisposition to increase the risk of developing psychopathology. In this work, we tested the hypothesis that social isolation stress interacts with impaired glutathione synthesis and have cumulative effects on the neurochemical profile of the frontal cortex. A mouse model with chronic glutathione deficit induced by knockout (-/-) of the glutamate-cysteine ligase modulatory subunit (Gclm) was exposed to social isolation stress from weaning to post-natal day 65. Using magnetic resonance methods at high-field (14.1 T), we analysed the neurochemical profile in the frontal cortex, brain size and ventricular volume of adult animals. Glutathione deficit was accompanied by elevated concentrations of N-acetylaspartate, alanine, and glutamine, as well as the ratio of glutamine-to-glutamate (Gln/Glu), and by a reduction in levels of myo-inositol and choline-containing compounds in the frontal cortex of -/- animals with respect to wild-type littermates. Although there was no significant interaction between social isolation stress and glutathione deficiency, mice reared in isolation displayed lower myo-inositol concentration (-8.4%, p social isolation had no effect on these parameters. We conclude that social isolation caused neurochemical alterations that may add to those associated to impaired glutathione synthesis. © 2017 International Society for Neurochemistry.

  20. The PANDA Barrel DIRC detector

    International Nuclear Information System (INIS)

    Hoek, M.; Dzhygadlo, R.; Gerhardt, A.; Götzen, K.; Hohler, R.; Kalicy, G.; Kumawat, H.; Lehmann, D.; Lewandowski, B.; Patsyuk, M.; Peters, K.; Schepers, G.; Schmitt, L.; Schwarz, C.; Schwiening, J.; Traxler, M.; Zühlsdorf, M.; Dodokhov, V. Kh.; Britting, A.; Eyrich, W.

    2014-01-01

    The PANDA experiment at the new Facility for Antiproton and Ion Research in Europe (FAIR) at GSI, Darmstadt, will study fundamental questions of hadron physics and QCD using high-intensity cooled antiproton beams with momenta between 1.5 and 15 GeV/c. Efficient Particle Identification for a wide momentum range and the full solid angle is required for reconstructing the various physics channels of the PANDA program. Hadronic Particle Identification in the barrel region of the detector will be provided by a DIRC counter. The design is based on the successful BABAR DIRC with important improvements, such as focusing optics and fast photon timing. Several of these improvements, including different radiator geometries and optics, were tested in particle beams at GSI and at CERN. The evolution of the conceptual design of the PANDA Barrel DIRC and the performance of complex prototypes in test beam campaigns will be discussed

  1. The PANDA Barrel DIRC detector

    Energy Technology Data Exchange (ETDEWEB)

    Hoek, M., E-mail: matthias.hoek@uni-mainz.de [Institut für Kernphysik, Johannes Gutenberg University Mainz, Mainz (Germany); Dzhygadlo, R.; Gerhardt, A.; Götzen, K.; Hohler, R.; Kalicy, G.; Kumawat, H.; Lehmann, D.; Lewandowski, B.; Patsyuk, M.; Peters, K.; Schepers, G.; Schmitt, L.; Schwarz, C.; Schwiening, J.; Traxler, M.; Zühlsdorf, M. [GSI Helmholtzzentrum für Schwerionenforschung GmbH, Darmstadt (Germany); Dodokhov, V. Kh. [Joint Institute for Nuclear Research, Dubna (Russian Federation); Britting, A.; Eyrich, W. [Friedrich Alexander-University of Erlangen-Nuremberg, Erlangen (Germany); and others

    2014-12-01

    The PANDA experiment at the new Facility for Antiproton and Ion Research in Europe (FAIR) at GSI, Darmstadt, will study fundamental questions of hadron physics and QCD using high-intensity cooled antiproton beams with momenta between 1.5 and 15 GeV/c. Efficient Particle Identification for a wide momentum range and the full solid angle is required for reconstructing the various physics channels of the PANDA program. Hadronic Particle Identification in the barrel region of the detector will be provided by a DIRC counter. The design is based on the successful BABAR DIRC with important improvements, such as focusing optics and fast photon timing. Several of these improvements, including different radiator geometries and optics, were tested in particle beams at GSI and at CERN. The evolution of the conceptual design of the PANDA Barrel DIRC and the performance of complex prototypes in test beam campaigns will be discussed.

  2. Chronic Stress Reduces Nectin-1 mRNA Levels and Disrupts Dendritic Spine Plasticity in the Adult Mouse Perirhinal Cortex

    Directory of Open Access Journals (Sweden)

    Qian Gong

    2018-03-01

    Full Text Available In adulthood, chronic exposure to stressful experiences disrupts synaptic plasticity and cognitive function. Previous studies have shown that perirhinal cortex-dependent object recognition memory is impaired by chronic stress. However, the stress effects on molecular expression and structural plasticity in the perirhinal cortex remain unclear. In this study, we applied the chronic social defeat stress (CSDS paradigm and measured the mRNA levels of nectin-1, nectin-3 and neurexin-1, three synaptic cell adhesion molecules (CAMs implicated in the adverse stress effects, in the perirhinal cortex of wild-type (WT and conditional forebrain corticotropin-releasing hormone receptor 1 conditional knockout (CRHR1-CKO mice. Chronic stress reduced perirhinal nectin-1 mRNA levels in WT but not CRHR1-CKO mice. In conditional forebrain corticotropin-releasing hormone conditional overexpression (CRH-COE mice, perirhinal nectin-1 mRNA levels were also reduced, indicating that chronic stress modulates nectin-1 expression through the CRH-CRHR1 system. Moreover, chronic stress altered dendritic spine morphology in the main apical dendrites and reduced spine density in the oblique apical dendrites of perirhinal layer V pyramidal neurons. Our data suggest that chronic stress disrupts cell adhesion and dendritic spine plasticity in perirhinal neurons, which may contribute to stress-induced impairments of perirhinal cortex-dependent memory.

  3. Preservation of primordial follicles from lions by slow freezing and xenotransplantation of ovarian cortex into an immunodeficient mouse

    DEFF Research Database (Denmark)

    Wiedemann, C; Hribal, R; Ringleb, J

    2012-01-01

    follicles within the ovarian cortex survived culture when the original sample was from a young healthy lion collected immediately after euthanasia. Within the xenotransplants, the number of primordial follicles decreased after 28 days by 20%, but the relation between primordial and growing follicles changed...

  4. Completion of the TRT Barrel

    CERN Multimedia

    Gagnon, P

    On February 3, the US-TRT team proudly completed the installation of the 96th barrel TRT module on its support structure in the SR building at CERN. This happy event came after many years of R&D initiated in the nineties by the TA1 team at CERN, followed by the construction of the modules in three American institutes (Duke, Hampton and Indiana Universities) from 1996 to 2003. In total, the 96 barrel modules contain 52544 kapton straws, each 4 mm in diameter and strung with a 30 micron gold-plated tungsten wire. Each wire was manually inserted, a feat in itself! The inner layer modules contain 329 straws, the middle layer modules have 520 straws and the outer layer, 793 straws. Thirty- two modules of each type form a full layer. Their special geometry was designed such as to leave no dead region. On average, a particle will cross 36 straws. Kirill Egorov, Chuck Mahlon and John Callahan inserted the last module in the Barrel Support Structure. After completion in the US, all modules were transferred...

  5. Functional testing of the ATLAS SCT barrels

    International Nuclear Information System (INIS)

    Phillips, Peter W.

    2007-01-01

    The ATLAS SCT (semiconductor tracker) comprises 2112 barrel modules mounted on four concentric barrels of length 1.6m and up to 1m diameter, and 1976 endcap modules supported by a series of nine wheels at each end of the barrel region, giving a total silicon area of 60m 2 . The assembly of modules onto each of the four barrel structures has recently been completed. In addition to functional tests made during the assembly process, each completed barrel was operated in its entirety. In the case of the largest barrel, with an active silicon area of approximately 10m 2 , this corresponds to more than one million instrumented channels. This paper documents the electrical performance of the four individual SCT barrels. An overview of the readout chain is also given

  6. Successive neuron loss in the thalamus and cortex in a mouse model of infantile neuronal ceroid lipofuscinosis.

    Science.gov (United States)

    Kielar, Catherine; Maddox, Lucy; Bible, Ellen; Pontikis, Charlie C; Macauley, Shannon L; Griffey, Megan A; Wong, Michael; Sands, Mark S; Cooper, Jonathan D

    2007-01-01

    Infantile neuronal ceroid lipofuscinosis (INCL) is caused by deficiency of the lysosomal enzyme, palmitoyl protein thioesterase 1 (PPT1). We have investigated the onset and progression of pathological changes in Ppt1 deficient mice (Ppt1-/-) and the development of their seizure phenotype. Surprisingly, cortical atrophy and neuron loss occurred only late in disease progression but were preceded by localized astrocytosis within individual thalamic nuclei and the progressive loss of thalamic neurons that relay different sensory modalities to the cortex. This thalamic neuron loss occurred first within the visual system and only subsequently in auditory and somatosensory relay nuclei or the inhibitory reticular thalamic nucleus. The loss of granule neurons and GABAergic interneurons followed in each corresponding cortical region, before the onset of seizure activity. These findings provide novel evidence for successive neuron loss within the thalamus and cortex in Ppt1-/- mice, revealing the thalamus as an important early focus of INCL pathogenesis.

  7. Exposure to brominated flame retardant PBDE-99 affects cytoskeletal protein expression in the neonatal mouse cerebral cortex

    DEFF Research Database (Denmark)

    Alm, Henrik; Kultima, Kim; Scholz, Birger

    2008-01-01

    , and the cytotoxic and apoptotic effects of PBDE-99 in primary cultures of fetal rat cortical cells. We used two-dimensional difference gel electrophoresis (2D-DIGE) to analyze protein samples isolated from the cortex of NMRI mice 24h after exposure to a single oral dose of 12 mg/kg PBDE-99 on post-natal day 10....... Protein resolution was enhanced by sample pre-fractionation. In the cell model, we determined cell viability using the trypan blue exclusion assay, and apoptosis using immunocytochemical detection of cleaved caspase-3. We determined the identity of 111 differentially expressed proteins, 32 (29%) of which...... are known to be cytoskeleton-related. Similar to previous findings in the striatum, we found elevated levels of the neuron growth-associated protein Gap43 in the cortex. In cultured cortical cells, a high concentration of PBDE-99 (30 microM) induced cell death without any apparent increase in caspase-3...

  8. Astrocytic cytoskeletal atrophy in the medial prefrontal cortex of a triple transgenic mouse model of Alzheimer's disease

    Czech Academy of Sciences Publication Activity Database

    Kulijewicz-Nawrot, Magdaléna; Verkhratsky, Alexei; Chvátal, Alexandr; Syková, Eva; Rodríguez Arellano, Jose Julio

    2012-01-01

    Roč. 221, č. 3 (2012), s. 252-262 ISSN 0021-8782 R&D Projects: GA ČR GA305/08/1384; GA ČR GA309/09/1696; GA ČR GAP304/11/0184 Institutional research plan: CEZ:AV0Z50390703 Institutional support: RVO:68378041 Keywords : Alzheimer’s disease,, * astroglia * medial prefrontal cortex Subject RIV: FH - Neurology Impact factor: 2.357, year: 2012

  9. Effects of noise-induced hearing loss on parvalbumin and perineuronal net expression in the mouse primary auditory cortex.

    Science.gov (United States)

    Nguyen, Anna; Khaleel, Haroun M; Razak, Khaleel A

    2017-07-01

    Noise induced hearing loss is associated with increased excitability in the central auditory system but the cellular correlates of such changes remain to be characterized. Here we tested the hypothesis that noise-induced hearing loss causes deterioration of perineuronal nets (PNNs) in the auditory cortex of mice. PNNs are specialized extracellular matrix components that commonly enwrap cortical parvalbumin (PV) containing GABAergic interneurons. Compared to somatosensory and visual cortex, relatively less is known about PV/PNN expression patterns in the primary auditory cortex (A1). Whether changes to cortical PNNs follow acoustic trauma remains unclear. The first aim of this study was to characterize PV/PNN expression in A1 of adult mice. PNNs increase excitability of PV+ inhibitory neurons and confer protection to these neurons against oxidative stress. Decreased PV/PNN expression may therefore lead to a reduction in cortical inhibition. The second aim of this study was to examine PV/PNN expression in superficial (I-IV) and deep cortical layers (V-VI) following noise trauma. Exposing mice to loud noise caused an increase in hearing threshold that lasted at least 30 days. PV and PNN expression in A1 was analyzed at 1, 10 and 30 days following the exposure. No significant changes were observed in the density of PV+, PNN+, or PV/PNN co-localized cells following hearing loss. However, a significant layer- and cell type-specific decrease in PNN intensity was seen following hearing loss. Some changes were present even at 1 day following noise exposure. Attenuation of PNN may contribute to changes in excitability in cortex following noise trauma. The regulation of PNN may open up a temporal window for altered excitability in the adult brain that is then stabilized at a new and potentially pathological level such as in tinnitus. Copyright © 2017 Elsevier B.V. All rights reserved.

  10. Preservation of Cognitive Function by Lepidium meyenii (Maca) Is Associated with Improvement of Mitochondrial Activity and Upregulation of Autophagy-Related Proteins in Middle-Aged Mouse Cortex.

    Science.gov (United States)

    Guo, Shan-Shan; Gao, Xiao-Fang; Gu, Yan-Rong; Wan, Zhong-Xiao; Lu, A-Ming; Qin, Zheng-Hong; Luo, Li

    2016-01-01

    Maca has been used as a foodstuff and a traditional medicine in the Andean region for over 2,000 years. Recently the neuroprotective effects of maca also arouse interest of researchers. Decrease in mitochondrial function and decline in autophagy signaling may participate in the process of age-related cognitive decline. This study aimed to investigate if maca could improve cognitive function of middle-aged mice and if this effect was associated with improvement of mitochondrial activity and modulation of autophagy signaling in mouse cortex. Fourteen-month-old male ICR mice received maca powder administered by gavage for five weeks. Maca improved cognitive function, motor coordination, and endurance capacity in middle-aged mice, accompanied by increased mitochondrial respiratory function and upregulation of autophagy-related proteins in cortex. Our findings suggest that maca is a newly defined nutritional plant which can improve mitochondrial function and upregulate autophagy-related proteins and may be an effective functional food for slowing down age-related cognitive decline.

  11. Preservation of Cognitive Function by Lepidium meyenii (Maca Is Associated with Improvement of Mitochondrial Activity and Upregulation of Autophagy-Related Proteins in Middle-Aged Mouse Cortex

    Directory of Open Access Journals (Sweden)

    Shan-Shan Guo

    2016-01-01

    Full Text Available Maca has been used as a foodstuff and a traditional medicine in the Andean region for over 2,000 years. Recently the neuroprotective effects of maca also arouse interest of researchers. Decrease in mitochondrial function and decline in autophagy signaling may participate in the process of age-related cognitive decline. This study aimed to investigate if maca could improve cognitive function of middle-aged mice and if this effect was associated with improvement of mitochondrial activity and modulation of autophagy signaling in mouse cortex. Fourteen-month-old male ICR mice received maca powder administered by gavage for five weeks. Maca improved cognitive function, motor coordination, and endurance capacity in middle-aged mice, accompanied by increased mitochondrial respiratory function and upregulation of autophagy-related proteins in cortex. Our findings suggest that maca is a newly defined nutritional plant which can improve mitochondrial function and upregulate autophagy-related proteins and may be an effective functional food for slowing down age-related cognitive decline.

  12. DELPHI Barrel Muon Chamber Module

    CERN Multimedia

    1989-01-01

    The module was used as part of the muon identification system on the barrel of the DELPHI detector at LEP, and was in active use from 1989 to 2000. The module consists of 7 individual muons chambers arranged in 2 layers. Chambers in the upper layer are staggered by half a chamber width with respect to the lower layer. Each individual chamber is a drift chamber consisting of an anode wire, 47 microns in diameter, and a wrapped copper delay line. Each chamber provided 3 signal for each muon passing through the chamber, from which a 3D space-point could be reconstructed.

  13. The OPAL muon barrel detector

    International Nuclear Information System (INIS)

    Akers, R.J.; Allison, J.; Ashton, P.; Bahan, G.A.; Baines, J.T.M.; Banks, J.N.; Barlow, R.J.; Barnett, S.; Beeston, C.; Chrin, J.T.M.; Clowes, S.G.; Davies, O.W.; Duerdoth, I.P.; Hinde, P.S.; Hughes-Jones, R.E.; Lafferty, G.D.; Loebinger, F.K.; Macbeth, A.A.; McGowan, R.F.; Moss, M.W.; Murphy, P.G.; Nijjhar, B.; O'Dowd, A.J.P.; Pawley, S.J.; Phillips, P.D.; Richards, G.E.; Skillman, A.; Stephens, K.; Tresillian, N.J.; Wood, N.C.; Wyatt, T.R.

    1995-01-01

    The barrel part of the OPAL muon detector consists of 110 drift chambers forming four layers outside the hadron absorber. Each chamber covers an area of 1.2 m by up to 10.4 m and has two cells with wires parallel to the beam and a drift distance of 297 mm. A detailed description of the design, construction, operation and performance of the sub-detector is given. The system has been operating successfully since the start of LEP in 1989. ((orig.))

  14. Paroxetine and Low-dose Risperidone Induce Serotonin 5-HT1A and Dopamine D2 Receptor Heteromerization in the Mouse Prefrontal Cortex.

    Science.gov (United States)

    Kolasa, Magdalena; Solich, Joanna; Faron-Górecka, Agata; Żurawek, Dariusz; Pabian, Paulina; Łukasiewicz, Sylwia; Kuśmider, Maciej; Szafran-Pilch, Kinga; Szlachta, Marta; Dziedzicka-Wasylewska, Marta

    2018-05-01

    Recently, it has been shown that serotonin 5-HT 1A receptor interacts with dopamine D2 receptor in vitro. However, the existence of 5-HT 1A -D2 heteromers in native tissue remains unexplored. In the present study, we investigated 5-HT 1A -D2 receptor heteromerization in mice treated acutely or chronically with paroxetine (10 mg/kg) or risperidone (0.05 mg/kg). Receptor heteromerization was visualized and quantified in the mouse brain by in situ proximity ligation assay (PLA). Additionally, we aimed to determine the cellular localization of 5-HT 1A -D2 receptor heteromers in mouse adult primary neuronal cells by immunofluorescent staining with markers for astrocytes (GFAP) and neurons (NeuN and MAP2). The results from the current study demonstrated that 5-HT 1A and D2 receptor co-localization and heteromerization occurred in the mouse prefrontal cortex. Counterstaining after PLA confirmed neuronal (pyramidal and GABAergic) as well as astrocytal localization of 5-HT 1A -D2 receptor heteromers. Chronic administration of paroxetine or risperidone increased the level of 5-HT 1A -D2 receptor heteromers in the prefrontal cortex. These changes were not accompanied by any changes in the expression of mRNAs (measured by in situ hybridization) or densities of 5-HT 1A and D2 receptors (quantified by receptor autoradiography with [3H]8-OH-DPAT and [3H]domperidone, respectively), what all indicated that paroxetine and risperidone facilitated 5-HT 1A -D2 heteromer formation independently of the receptor expression. In vitro homogenous time-resolved FRET (HTRF) study confirmed the ability of tested drugs to influence the human 5-HT 1A -D2 heteromer formation. The obtained data indicate that the increase in 5-HT 1A -D2 receptor heteromerization is a common molecular characteristic of paroxetine and low-dose risperidone treatment. Copyright © 2018 IBRO. Published by Elsevier Ltd. All rights reserved.

  15. A Mouse Model of Visual Perceptual Learning Reveals Alterations in Neuronal Coding and Dendritic Spine Density in the Visual Cortex

    OpenAIRE

    Wang, Yan; Wu, Wei; Zhang, Xian; Hu, Xu; Li, Yue; Lou, Shihao; Ma, Xiao; An, Xu; Liu, Hui; Peng, Jing; Ma, Danyi; Zhou, Yifeng; Yang, Yupeng

    2016-01-01

    Visual perceptual learning (VPL) can improve spatial vision in normally sighted and visually impaired individuals. Although previous studies of humans and large animals have explored the neural basis of VPL, elucidation of the underlying cellular and molecular mechanisms remains a challenge. Owing to the advantages of molecular genetic and optogenetic manipulations, the mouse is a promising model for providing a mechanistic understanding of VPL. Here, we thoroughly evaluated the effects and p...

  16. Classic cadherin expressions balance postnatal neuronal positioning and dendrite dynamics to elaborate the specific cytoarchitecture of the mouse cortical area.

    Science.gov (United States)

    Egusa, Saki F; Inoue, Yukiko U; Asami, Junko; Terakawa, Youhei W; Hoshino, Mikio; Inoue, Takayoshi

    2016-04-01

    A unique feature of the mammalian cerebral cortex is in its tangential parcellation via anatomical and functional differences. However, the cellular and/or molecular machinery involved in cortical arealization remain largely unknown. Here we map expression profiles of classic cadherins in the postnatal mouse barrel field of the primary somatosensory area (S1BF) and generate a novel bacterial artificial chromosome transgenic (BAC-Tg) mouse line selectively illuminating nuclei of cadherin-6 (Cdh6)-expressing layer IV barrel neurons to confirm that tangential cellular assemblage of S1BF is established by postnatal day 5 (P5). When we electroporate the cadherins expressed in both barrel neurons and thalamo-cortical axon (TCA) terminals limited to the postnatal layer IV neurons, S1BF cytoarchitecture is disorganized with excess elongation of dendrites at P7. Upon delivery of dominant negative molecules for all classic cadherins, tangential cellular positioning and biased dendritic arborization of barrel neurons are significantly altered. These results underscore the value of classic cadherin-mediated sorting among neuronal cell bodies, dendrites and TCA terminals in postnatally elaborating the S1BF-specific tangential cytoarchitecture. Additionally, how the "protocortex" machinery affects classic cadherin expression profiles in the process of cortical arealization is examined and discussed. Copyright © 2015 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.

  17. TRT and SCT barrels merge

    CERN Multimedia

    Wells, P S

    2006-01-01

    The SCT barrel was inserted in the TRT on 17 February, just missing Valentine's day. This was a change of emphasis for the two detectors. In the preceeding months there had been a lot of focus on testing their performance. The TRT had been observing cosmic rays through several sectors of the barrel, and all the modules on each of the four layers of the SCT had been characterised prior to integration. In parallel, the engineering teams, lead by Marco Olcese, Andrea Catinaccio, Eric Perrin, Neil Dixon, Iourii Gusakov, Gerard Barbier and Takashi Kohriki, had been preparing for this critical operation. Figure 1: Neil Dixon and Marco Olcese verifying the final alignment The two detectors had to be painstakingly aligned to be concentric to within a millimetre. The SCT was held on a temporary cantilever stand, and the TRT in the ID trolley had to inch over it. Finally the weight of the SCT was transferred to the rails on the inside of the TRT itself. The SCT services actually protruded a little outside the oute...

  18. Developmental and visual input-dependent regulation of the CB1 cannabinoid receptor in the mouse visual cortex.

    Directory of Open Access Journals (Sweden)

    Taisuke Yoneda

    Full Text Available The mammalian visual system exhibits significant experience-induced plasticity in the early postnatal period. While physiological studies have revealed the contribution of the CB1 cannabinoid receptor (CB1 to developmental plasticity in the primary visual cortex (V1, it remains unknown whether the expression and localization of CB1 is regulated during development or by visual experience. To explore a possible role of the endocannabinoid system in visual cortical plasticity, we examined the expression of CB1 in the visual cortex of mice. We found intense CB1 immunoreactivity in layers II/III and VI. CB1 mainly localized at vesicular GABA transporter-positive inhibitory nerve terminals. The amount of CB1 protein increased throughout development, and the specific laminar pattern of CB1 appeared at P20 and remained until adulthood. Dark rearing from birth to P30 decreased the amount of CB1 protein in V1 and altered the synaptic localization of CB1 in the deep layer. Dark rearing until P50, however, did not influence the expression of CB1. Brief monocular deprivation for 2 days upregulated the localization of CB1 at inhibitory nerve terminals in the deep layer. Taken together, the expression and the localization of CB1 are developmentally regulated, and both parameters are influenced by visual experience.

  19. Post-Stroke Longitudinal Alterations of Inter-Hemispheric Correlation and Hemispheric Dominance in Mouse Pre-Motor Cortex.

    Science.gov (United States)

    Vallone, Fabio; Lai, Stefano; Spalletti, Cristina; Panarese, Alessandro; Alia, Claudia; Micera, Silvestro; Caleo, Matteo; Di Garbo, Angelo

    2016-01-01

    Limited restoration of function is known to occur spontaneously after an ischemic injury to the primary motor cortex. Evidence suggests that Pre-Motor Areas (PMAs) may "take over" control of the disrupted functions. However, little is known about functional reorganizations in PMAs. Forelimb movements in mice can be driven by two cortical regions, Caudal and Rostral Forelimb Areas (CFA and RFA), generally accepted as primary motor and pre-motor cortex, respectively. Here, we examined longitudinal changes in functional coupling between the two RFAs following unilateral photothrombotic stroke in CFA (mm from Bregma: +0.5 anterior, +1.25 lateral). Local field potentials (LFPs) were recorded from the RFAs of both hemispheres in freely moving injured and naïve mice. Neural signals were acquired at 9, 16 and 23 days after surgery (sub-acute period in stroke animals) through one bipolar electrode per hemisphere placed in the center of RFA, with a ground screw over the occipital bone. LFPs were pre-processed through an efficient method of artifact removal and analysed through: spectral,cross-correlation, mutual information and Granger causality analysis. Spectral analysis demonstrated an early decrease (day 9) in the alpha band power in both the RFAs. In the late sub-acute period (days 16 and 23), inter-hemispheric functional coupling was reduced in ischemic animals, as shown by a decrease in the cross-correlation and mutual information measures. Within the gamma and delta bands, correlation measures were already reduced at day 9. Granger analysis, used as a measure of the symmetry of the inter-hemispheric causal connectivity, showed a less balanced activity in the two RFAs after stroke, with more frequent oscillations of hemispheric dominance. These results indicate robust electrophysiological changes in PMAs after stroke. Specifically, we found alterations in transcallosal connectivity, with reduced inter-hemispheric functional coupling and a fluctuating dominance

  20. Post-Stroke Longitudinal Alterations of Inter-Hemispheric Correlation and Hemispheric Dominance in Mouse Pre-Motor Cortex.

    Directory of Open Access Journals (Sweden)

    Fabio Vallone

    Full Text Available Limited restoration of function is known to occur spontaneously after an ischemic injury to the primary motor cortex. Evidence suggests that Pre-Motor Areas (PMAs may "take over" control of the disrupted functions. However, little is known about functional reorganizations in PMAs. Forelimb movements in mice can be driven by two cortical regions, Caudal and Rostral Forelimb Areas (CFA and RFA, generally accepted as primary motor and pre-motor cortex, respectively. Here, we examined longitudinal changes in functional coupling between the two RFAs following unilateral photothrombotic stroke in CFA (mm from Bregma: +0.5 anterior, +1.25 lateral.Local field potentials (LFPs were recorded from the RFAs of both hemispheres in freely moving injured and naïve mice. Neural signals were acquired at 9, 16 and 23 days after surgery (sub-acute period in stroke animals through one bipolar electrode per hemisphere placed in the center of RFA, with a ground screw over the occipital bone. LFPs were pre-processed through an efficient method of artifact removal and analysed through: spectral,cross-correlation, mutual information and Granger causality analysis.Spectral analysis demonstrated an early decrease (day 9 in the alpha band power in both the RFAs. In the late sub-acute period (days 16 and 23, inter-hemispheric functional coupling was reduced in ischemic animals, as shown by a decrease in the cross-correlation and mutual information measures. Within the gamma and delta bands, correlation measures were already reduced at day 9. Granger analysis, used as a measure of the symmetry of the inter-hemispheric causal connectivity, showed a less balanced activity in the two RFAs after stroke, with more frequent oscillations of hemispheric dominance.These results indicate robust electrophysiological changes in PMAs after stroke. Specifically, we found alterations in transcallosal connectivity, with reduced inter-hemispheric functional coupling and a fluctuating

  1. Peripheral nerve injury is associated with chronic, reversible changes in global DNA methylation in the mouse prefrontal cortex.

    Directory of Open Access Journals (Sweden)

    Maral Tajerian

    Full Text Available Changes in brain structure and cortical function are associated with many chronic pain conditions including low back pain and fibromyalgia. The magnitude of these changes correlates with the duration and/or the intensity of chronic pain. Most studies report changes in common areas involved in pain modulation, including the prefrontal cortex (PFC, and pain-related pathological changes in the PFC can be reversed with effective treatment. While the mechanisms underlying these changes are unknown, they must be dynamically regulated. Epigenetic modulation of gene expression in response to experience and environment is reversible and dynamic. Epigenetic modulation by DNA methylation is associated with abnormal behavior and pathological gene expression in the central nervous system. DNA methylation might also be involved in mediating the pathologies associated with chronic pain in the brain. We therefore tested a whether alterations in DNA methylation are found in the brain long after chronic neuropathic pain is induced in the periphery using the spared nerve injury modal and b whether these injury-associated changes are reversible by interventions that reverse the pathologies associated with chronic pain. Six months following peripheral nerve injury, abnormal sensory thresholds and increased anxiety were accompanied by decreased global methylation in the PFC and the amygdala but not in the visual cortex or the thalamus. Environmental enrichment attenuated nerve injury-induced hypersensitivity and reversed the changes in global PFC methylation. Furthermore, global PFC methylation correlated with mechanical and thermal sensitivity in neuropathic mice. In summary, induction of chronic pain by peripheral nerve injury is associated with epigenetic changes in the brain. These changes are detected long after the original injury, at a long distance from the site of injury and are reversible with environmental manipulation. Changes in brain structure and

  2. Core barrel motion calibration factor calculation

    International Nuclear Information System (INIS)

    Shahrokhi, F.; Robinson, J.C.

    1976-01-01

    Neutron transport theory calculations were performed to obtain a calibration factor for inferring core-barrel motion from spectral density data using excore ionization chambers in PWRs. The analysis of core-barrel movement was based on the postulate that the movement is a cantilevered type, with the preferred direction x-x'

  3. Prototyping the PANDA Barrel DIRC

    Energy Technology Data Exchange (ETDEWEB)

    Schwarz, C., E-mail: C.Schwarz@gsi.de [GSI Helmholtzzentrum für Schwerionenforschung GmbH, Darmstadt (Germany); Kalicy, G.; Dzhygadlo, R.; Gerhardt, A.; Götzen, K.; Hohler, R.; Kumawat, H.; Lehmann, D.; Lewandowski, B.; Patsyuk, M.; Peters, K.; Schepers, G.; Schmitt, L.; Schwiening, J.; Traxler, M.; Zühlsdorf, M. [GSI Helmholtzzentrum für Schwerionenforschung GmbH, Darmstadt (Germany); Dodokhov, V.Kh. [Joint Institute for Nuclear Research, Dubna (Russian Federation); Britting, A.; Eyrich, W.; Lehmann, A. [Friedrich Alexander-University of Erlangen-Nuremberg, Erlangen (Germany); and others

    2014-12-01

    The design of the Barrel DIRC detector for the future PANDA experiment at FAIR contains several important improvements compared to the successful BABAR DIRC, such as focusing and fast timing. To test those improvements as well as other design options a prototype was build and successfully tested in 2012 with particle beams at CERN. The prototype comprises a radiator bar, focusing lens, mirror, and a prism shaped expansion volume made of synthetic fused silica. An array of micro-channel plate photomultiplier tubes measures the location and arrival time of the Cherenkov photons with sub-nanosecond resolution. The development of a fast reconstruction algorithm allowed to tune construction details of the detector setup with test beam data and Monte-Carlo simulations.

  4. Characterization of excitatory and inhibitory neuron activation in the mouse medial prefrontal cortex following palatable food ingestion and food driven exploratory behavior

    Directory of Open Access Journals (Sweden)

    Ronald P Gaykema

    2014-07-01

    Full Text Available The medial prefrontal cortex (mPFC is implicated in aspects of executive function, that include the modulation of attentional and memory processes involved in goal selection. Food-seeking behavior has been shown to involve activation of the mPFC, both during the execution of strategies designed to obtain food and during the consumption of food itself. As these behaviors likely require differential engagement of the prefrontal cortex, we hypothesized that the pattern of neuronal activation would also be behavior dependent. In this study we describe, for the first time, the expression of Fos in different layers and cell types of the infralimbic/dorsal peduncular (IL/DP and prelimbic/anterior cingulate (PL/AC subdivisions of mouse mPFC following both the consumption of palatable food and following exploratory activity of the animal directed at obtaining food reward. While both manipulations led to increases of Fos expression in principal excitatory neurons relative to control, food-directed exploratory activity produced a significantly greater increase in Fos expression than observed in the food intake condition. Consequently, we hypothesized that mPFC interneuron activation would also be differentially engaged by these manipulations. Interestingly, Fos expression patterns differed substantially between treatments and interneuron subtype, illustrating how the differential engagement of subsets of mPFC interneurons depends on the behavioral state. In our experiments, both vasoactive intestinal peptide- and parvalbumin-expressing neurons showed enhanced Fos expression only during the food-dependent exploratory task and not during food intake. Conversely, elevations in arcuate and paraventricular hypothalamic fos expression were only observed following food intake and not following food driven exploration. Our data suggest that activation of select mPFC interneurons may be required to support high cognitive demand states while being dispensable during

  5. The effect of extracts of Searsia species on epileptiform activity in slices of the mouse cerebral cortex

    DEFF Research Database (Denmark)

    Pedersen, Mikael Egebjerg; Vestergaard, Henrik Tang; Stafford, Gary Ivan

    2008-01-01

    ETHNOPHARMACOLOGICAL RELEVANCE: Searsia dentata and Searsia pyroides are used in traditional South African medicine to treat convulsions and epilepsy. Previous studies have demonstrated that extracts of these plants comprise compounds that bind to the flumazenil-sensitive site on the GABA(A) rece...... of the crude ethanolic extracts of these two South African medicinal plants was demonstrated.......ETHNOPHARMACOLOGICAL RELEVANCE: Searsia dentata and Searsia pyroides are used in traditional South African medicine to treat convulsions and epilepsy. Previous studies have demonstrated that extracts of these plants comprise compounds that bind to the flumazenil-sensitive site on the GABA......(A) receptor. However, their use as anticonvulsant medicinal plants cannot be adequately explained by these findings. AIMS: The aim of this study was to examine the possible involvement of the glutamatergic system of extracts from the plants. MATERIALS AND METHODS: The mouse cortical wedge preparation was used...

  6. Demonstration of neuron-glia transfer of precursors for GABA biosynthesis in a co-culture system of dissociated mouse cerebral cortex.

    Science.gov (United States)

    Leke, Renata; Bak, Lasse K; Schousboe, Arne; Waagepetersen, Helle S

    2008-12-01

    Co-cultures of neurons and astrocytes were prepared from dissociated embryonic mouse cerebral cortex and cultured for 7 days. To investigate if these cultures may serve as a functional model system to study neuron-glia interaction with regard to GABA biosynthesis, the cells were incubated either in media containing [U-(13)C]glutamine (0.1, 0.3 and 0.5 mM) or 1 mM acetate plus 2.5 mM glucose plus 1 mM lactate. In the latter case one of the 3 substrates was uniformly (13)C labeled. Cellular contents and (13)C labeling of glutamate, GABA, aspartate and glutamine were determined in the cells after an incubation period of 2.5 h. The GABA biosynthetic machinery exhibited the expected complexity with regard to metabolic compartmentation and involvement of TCA cycle activity as seen in other culture systems containing GABAergic neurons. Metabolism of acetate clearly demonstrated glial synthesis of glutamine and its transfer to the neuronal compartment. It is concluded that this co-culture system serves as a reliable model in which functional and pharmacological aspects of GABA biosynthesis can be investigated.

  7. H3 and H4 Lysine Acetylation Correlates with Developmental and Experimentally Induced Adult Experience-Dependent Plasticity in the Mouse Visual Cortex

    Directory of Open Access Journals (Sweden)

    Gabriela Vierci

    2016-01-01

    Full Text Available Histone posttranslational modifications play a fundamental role in orchestrating gene expression. In this work, we analyzed the acetylation of H3 and H4 histones (AcH3-AcH4 and its modulation by visual experience in the mouse visual cortex (VC during normal development and in two experimental conditions that restore juvenile-like plasticity levels in adults (fluoxetine treatment and enriched environment. We found that AcH3-AcH4 declines with age and is upregulated by treatments restoring plasticity in the adult. We also found that visual experience modulates AcH3-AcH4 in young and adult plasticity-restored mice but not in untreated ones. Finally, we showed that the transporter vGAT is downregulated in adult plasticity-restored models. In summary, we identified a dynamic regulation of AcH3-AcH4, which is associated with high plasticity levels and enhanced by visual experience. These data, along with recent ones, indicate H3-H4 acetylation as a central hub in the control of experience-dependent plasticity in the VC.

  8. Age-Dependent Long-Term Potentiation Deficits in the Prefrontal Cortex of the Fmr1 Knockout Mouse Model of Fragile X Syndrome.

    Science.gov (United States)

    Martin, Henry G S; Lassalle, Olivier; Brown, Jonathan T; Manzoni, Olivier J

    2016-05-01

    The most common inherited monogenetic cause of intellectual disability is Fragile X syndrome (FXS). The clinical symptoms of FXS evolve with age during adulthood; however, neurophysiological data exploring this phenomenon are limited. The Fmr1 knockout (Fmr1KO) mouse models FXS, but studies in these mice of prefrontal cortex (PFC) function are underrepresented, and aging linked data are absent. We studied synaptic physiology and activity-dependent synaptic plasticity in the medial PFC of Fmr1KO mice from 2 to 12 months. In young adult Fmr1KO mice, NMDA receptor (NMDAR)-mediated long-term potentiation (LTP) is intact; however, in 12-month-old mice this LTP is impaired. In parallel, there was an increase in the AMPAR/NMDAR ratio and a concomitant decrease of synaptic NMDAR currents in 12-month-old Fmr1KO mice. We found that acute pharmacological blockade of mGlu5 receptor in 12-month-old Fmr1KO mice restored a normal AMPAR/NMDAR ratio and LTP. Taken together, the data reveal an age-dependent deficit in LTP in Fmr1KO mice, which may correlate to some of the complex age-related deficits in FXS. © The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  9. Phase-specific Surround suppression in Mouse Primary Visual Cortex Correlates with Figure Detection Behavior Based on Phase Discontinuity.

    Science.gov (United States)

    Li, Fengling; Jiang, Weiqian; Wang, Tian-Yi; Xie, Taorong; Yao, Haishan

    2018-05-21

    In the primary visual cortex (V1), neuronal responses to stimuli within the receptive field (RF) are modulated by stimuli in the RF surround. A common effect of surround modulation is surround suppression, which is dependent on the feature difference between stimuli within and surround the RF and is suggested to be involved in the perceptual phenomenon of figure-ground segregation. In this study, we examined the relationship between feature-specific surround suppression of V1 neurons and figure detection behavior based on figure-ground feature difference. We trained freely moving mice to perform a figure detection task using figure and ground gratings that differed in spatial phase. The performance of figure detection increased with the figure-ground phase difference, and was modulated by stimulus contrast. Electrophysiological recordings from V1 in head-fixed mice showed that the increase in phase difference between stimuli within and surround the RF caused a reduction in surround suppression, which was associated with an increase in V1 neural discrimination between stimuli with and without RF-surround phase difference. Consistent with the behavioral performance, the sensitivity of V1 neurons to RF-surround phase difference could be influenced by stimulus contrast. Furthermore, inhibiting V1 by optogenetically activating either parvalbumin (PV)- or somatostatin (SOM)-expressing inhibitory neurons both decreased the behavioral performance of figure detection. Thus, the phase-specific surround suppression in V1 represents a neural correlate of figure detection behavior based on figure-ground phase discontinuity. Copyright © 2018 IBRO. Published by Elsevier Ltd. All rights reserved.

  10. Synaptic responses evoked by tactile stimuli in Purkinje cells in mouse cerebellar cortex Crus II in vivo.

    Directory of Open Access Journals (Sweden)

    Chun-Ping Chu

    Full Text Available Sensory stimuli evoke responses in cerebellar Purkinje cells (PCs via the mossy fiber-granule cell pathway. However, the properties of synaptic responses evoked by tactile stimulation in cerebellar PCs are unknown. The present study investigated the synaptic responses of PCs in response to an air-puff stimulation on the ipsilateral whisker pad in urethane-anesthetized mice.Thirty-three PCs were recorded from 48 urethane-anesthetized adult (6-8-week-old HA/ICR mice by somatic or dendritic patch-clamp recording and pharmacological methods. Tactile stimulation to the ipsilateral whisker pad was delivered by an air-puff through a 12-gauge stainless steel tube connected with a pressurized injection system. Under current-clamp conditions (I = 0, the air-puff stimulation evoked strong inhibitory postsynaptic potentials (IPSPs in the somata of PCs. Application of SR95531, a specific GABA(A receptor antagonist, blocked IPSPs and revealed stimulation-evoked simple spike firing. Under voltage-clamp conditions, tactile stimulation evoked a sequence of transient inward currents followed by strong outward currents in the somata and dendrites in PCs. Application of SR95531 blocked outward currents and revealed excitatory postsynaptic currents (EPSCs in somata and a temporal summation of parallel fiber EPSCs in PC dendrites. We also demonstrated that PCs respond to both the onset and offset of the air-puff stimulation.These findings indicated that tactile stimulation induced asynchronous parallel fiber excitatory inputs onto the dendrites of PCs, and failed to evoke strong EPSCs and spike firing in PCs, but induced the rapid activation of strong GABA(A receptor-mediated inhibitory postsynaptic currents in the somata and dendrites of PCs in the cerebellar cortex Crus II in urethane-anesthetized mice.

  11. Effects of Chronic Sleep Restriction during Early Adolescence on the Adult Pattern of Connectivity of Mouse Secondary Motor Cortex123

    Science.gov (United States)

    Billeh, Yazan N.; Bernard, Amy; de Vivo, Luisa; Honjoh, Sakiko; Mihalas, Stefan; Ng, Lydia; Koch, Christof

    2016-01-01

    Abstract Cortical circuits mature in stages, from early synaptogenesis and synaptic pruning to late synaptic refinement, resulting in the adult anatomical connection matrix. Because the mature matrix is largely fixed, genetic or environmental factors interfering with its establishment can have irreversible effects. Sleep disruption is rarely considered among those factors, and previous studies have focused on very young animals and the acute effects of sleep deprivation on neuronal morphology and cortical plasticity. Adolescence is a sensitive time for brain remodeling, yet whether chronic sleep restriction (CSR) during adolescence has long-term effects on brain connectivity remains unclear. We used viral-mediated axonal labeling and serial two-photon tomography to measure brain-wide projections from secondary motor cortex (MOs), a high-order area with diffuse projections. For each MOs target, we calculated the projection fraction, a combined measure of passing fibers and axonal terminals normalized for the size of each target. We found no homogeneous differences in MOs projection fraction between mice subjected to 5 days of CSR during early adolescence (P25–P30, ≥50% decrease in daily sleep, n=14) and siblings that slept undisturbed (n=14). Machine learning algorithms, however, classified animals at significantly above chance levels, indicating that differences between the two groups exist, but are subtle and heterogeneous. Thus, sleep disruption in early adolescence may affect adult brain connectivity. However, because our method relies on a global measure of projection density and was not previously used to measure connectivity changes due to behavioral manipulations, definitive conclusions on the long-term structural effects of early CSR require additional experiments. PMID:27351022

  12. Chronic Alcohol Consumption Alters Mammalian Target of Rapamycin (mTOR), Reduces Ribosomal p70S6 Kinase and p4E-BP1 Levels in Mouse Cerebral Cortex

    OpenAIRE

    Li, Qun; Ren, Jun

    2007-01-01

    Reduced insulin sensitivity following chronic alcohol consumption may contribute to alcohol-induced brain damage although the underlying mechanism(s) has not been elucidated. This study was designed to examine the effect of chronic alcohol intake on insulin signaling in mouse cerebral cortex. FVB mice were fed with a 4% alcohol diet for 16 weeks. Insulin receptor substrates (IRS-1, IRS-2) and post-receptor signaling molecules Akt, mammalian target of rapamycin (mTOR), ribosomal p70s6 kinase (...

  13. Liquid Argon Barrel Cryostat Arrived

    CERN Multimedia

    Pailler, P

    Last week the first of three cryostats for the ATLAS liquid argon calorimeter arrived at CERN. It had travelled for 46 days over several thousand kilometers from Japan to CERN. During three years it has been fabricated by Kawasaki Heavy Industries Ltd. at Harima, close to Kobe, under contract from Brookhaven National Laboratory (BNL) of the U.S.. This cryostat consists of two concentric cylinders made of aluminium: the outer vacuum vessel with a diameter of 5.5 m and a length of 7 m, and the inner cold vessel which will contain the electromagnetic barrel calorimeter immersed in liquid argon. The total weight will be 270 tons including the detectors and the liquid argon. The cryostat is now located in building 180 where it will be equipped with 64 feed-throughs which serve for the passage of 122,880 electrical lines which will carry the signals of the calorimeter. After integration of the calorimeter, the solenoidal magnet of ATLAS will be integrated in the vacuum vessel. A final cold test of the cryostat inc...

  14. Celebrating the Barrel Toroid commissioning

    CERN Multimedia

    Peter Jenni

    ATLAS invited Funding Agency representatives and Laboratory Heads directly related to the funding and construction of the Barrel Toroid for a small ceremony on 13th December 2006 at Point 1, in order to mark the successful first full excitation of the BT (see last eNews). On that date, which was during the December CERN Council week, several of the Funding Agency Heads or their representatives could be present, representing CEA France, INFN Italy, BMBF Germany, Spain, Sweden, Switzerland, Russia, JINR Dubna and CERN. Speeches were delivered by the ATLAS spokesperson Peter Jenni thanking the Funding Partners in the name of the Collaboration, by Magnet Project Leader Herman ten Kate tracing the BT construction history, and by the CERN Director-General Robert Aymar congratulating all those who have contributed to the successful project. Herman ten Kate addressing the delegates. The text of the introductory address by Peter Jenni is reproduced here. "It is a great pleasure for me to welcome you all here...

  15. Understanding and Predicting Gun Barrel Erosion

    National Research Council Canada - National Science Library

    Johnston, Ian A

    2005-01-01

    The Australian Defence Force will soon have to contend with gun barrel erosion issues arising from the use of new low-vulnerability gun propellants, the acquisition of new ammunition and gun systems...

  16. Aerobic Glycolysis in the Frontal Cortex Correlates with Memory Performance in Wild-Type Mice But Not the APP/PS1 Mouse Model of Cerebral Amyloidosis.

    Science.gov (United States)

    Harris, Richard A; Tindale, Lauren; Lone, Asad; Singh, Olivia; Macauley, Shannon L; Stanley, Molly; Holtzman, David M; Bartha, Robert; Cumming, Robert C

    2016-02-10

    Aerobic glycolysis and lactate production in the brain plays a key role in memory, yet the role of this metabolism in the cognitive decline associated with Alzheimer's disease (AD) remains poorly understood. Here we examined the relationship between cerebral lactate levels and memory performance in an APP/PS1 mouse model of AD, which progressively accumulates amyloid-β. In vivo (1)H-magnetic resonance spectroscopy revealed an age-dependent decline in lactate levels within the frontal cortex of control mice, whereas lactate levels remained unaltered in APP/PS1 mice from 3 to 12 months of age. Analysis of hippocampal interstitial fluid by in vivo microdialysis revealed a significant elevation in lactate levels in APP/PS1 mice relative to control mice at 12 months of age. An age-dependent decline in the levels of key aerobic glycolysis enzymes and a concomitant increase in lactate transporter expression was detected in control mice. Increased expression of lactate-producing enzymes correlated with improved memory in control mice. Interestingly, in APP/PS1 mice the opposite effect was detected. In these mice, increased expression of lactate producing enzymes correlated with poorer memory performance. Immunofluorescent staining revealed localization of the aerobic glycolysis enzymes pyruvate dehydrogenase kinase and lactate dehydrogenase A within cortical and hippocampal neurons in control mice, as well as within astrocytes surrounding amyloid plaques in APP/PS1 mice. These observations collectively indicate that production of lactate, via aerobic glycolysis, is beneficial for memory function during normal aging. However, elevated lactate levels in APP/PS1 mice indicate perturbed lactate processing, a factor that may contribute to cognitive decline in AD. Lactate has recently emerged as a key metabolite necessary for memory consolidation. Lactate is the end product of aerobic glycolysis, a unique form of metabolism that occurs within certain regions of the brain. Here

  17. Work on the ATLAS semiconductor tracker barrel

    CERN Multimedia

    Maximilien Brice

    2005-01-01

    Precision work is performed on the semiconductor tracker barrel of the ATLAS experiment. All work on these delicate components must be performed in a clean room so that impurities in the air, such as dust, do not contaminate the detector. The semiconductor tracker will be mounted in the barrel close to the heart of the ATLAS experiment to detect the path of particles produced in proton-proton collisions.

  18. The TileCal Barrel Test Assembly

    CERN Multimedia

    Leitner, R

    On 30th October, the mechanics test assembly of the central barrel of the ATLAS tile hadronic calorimeter was completed in building 185. It started on 23rd June and is the second wheel for the Tilecal completely assembled this year. The ATLAS engineers and technicians are quick: instead of the 27 weeks initially foreseen for assembling the central barrel of the tile hadronic calorimeter (Tilecal) in building 185, they inserted the last of the 64 modules on 30th October after only 19 weeks. In part, this was due to the experience gained in the dry run assembly of the first extended barrel, produced in Spain, in spring this year (see Bulletin 23/2003); however, the central barrel is twice as long - and twice as heavy. With a length of 6.4 metres, an outer diameter of 8.5 metres and an inner diameter of 4.5 metres, the object weight is 1300 tonnes. The whole barrel cylinder is supported by the stainless steel support structure weighing only 27 tons. The barrel also has to have the right shape: over the whole 8...

  19. First SCT Barrel arrives at CERN

    CERN Multimedia

    Apsimon, R

    Mid-January saw the arrival at CERN of Barrel #3, the first of four SCT barrels. The barrels are formed as low-mass cylinders of carbon fibre skins on a honeycomb carbon core. They are manufactured in industry and then have all the final precision supports added and the final geometric metrology carried out at Geneva University. Barrel #3, complete with its 384 silicon detector modules, arrived by road from Oxford University in England where the modules were mounted using a purpose-built robot. The modules had been selected from the output of all four barrel module building clusters (in Japan, Scandinavia, USA and the UK). Since Barrel #3 will be exposed to high radiation levels within the tracker volume, these modules, representing over half a million readout channels, have been extensively tested at their operational temperature of around -25 degrees Celcius and at voltages of up to 500V. The dangers of shipping such a fragile component of ATLAS were apparent to all and considerable attention was focused...

  20. Processing of Niobium-Lined M240 Machine Gun Barrels

    Science.gov (United States)

    2014-11-01

    Fig. 5 Finished niobium-lined M240 machine gun barrel with flash suppressor attached ..........11 Fig. 6 End of barrel 1 showing small amount of...the finished barrel is shown in Fig. 5. 11 Fig. 5 Finished niobium-lined M240 machine gun barrel with flash suppressor attached Firing tests

  1. Activation of pyramidal neurons in mouse medial prefrontal cortex enhances food seeking behavior while reducing impulsivity in the absence of an effect on food intake

    Directory of Open Access Journals (Sweden)

    Daniel McAllister Warthen

    2016-03-01

    Full Text Available The medial prefrontal cortex (mPFC is involved in a wide range of executive cognitive functions, including reward evaluation, decision-making, memory extinction, mood, and task switching. Manipulation of the mPFC has been shown to alter food intake and food reward valuation, but whether exclusive stimulation of mPFC pyramidal neurons, which form the principle output of the mPFC, is sufficient to mediate food rewarded instrumental behavior is unknown. We sought to determine the behavioral consequences of manipulating mPFC output by exciting pyramidal neurons in mouse mPFC during performance of a panel of behavioral assays, focusing on food reward. We found that increasing mPFC pyramidal cell output using Designer Receptors Exclusively Activated by Designer Drugs (DREADD enhanced performance in instrumental food reward assays that assess food seeking behavior, while sparing effects in affect and food intake. Specifically, activation of mPFC pyramidal neurons enhanced operant responding for food reward, reinstatement of palatable food seeking, and suppression of impulsive responding for food reward. Conversely, activation of mPFC pyramidal neurons had no effect on unconditioned food intake, social interaction, or behavior in an open field. Furthermore, we found that behavioral outcome is influenced by the degree of mPFC activation, with a low drive sufficient to enhance operant responding and a higher drive required to alter impulsivity. Additionally, we provide data demonstrating that DREADD stimulation involves a nitric oxide synthase dependent pathway, similar to endogenous muscarinic M3 receptor stimulation, a finding that provides novel mechanistic insight into an increasingly widespread method of remote neuronal control.

  2. Evidence for proteolytic cleavage of brevican by the ADAMTSs in the dentate gyrus after excitotoxic lesion of the mouse entorhinal cortex

    Directory of Open Access Journals (Sweden)

    Gottschall Paul E

    2005-08-01

    Full Text Available Abstract Background Brevican is a member of the lectican family of aggregating extracellular matrix (ECM proteoglycans that bear chondroitin sulfate (CS chains. It is highly expressed in the central nervous system (CNS and is thought to stabilize synapses and inhibit neural plasticity and as such, neuritic or synaptic remodeling would be less likely to occur in regions with intact and abundant, lectican-containing, ECM complexes. Neural plasticity may occur more readily when these ECM complexes are broken down by endogenous proteases, the ADAMTSs (adisintegrin and metalloproteinase with thrombospondin motifs, that selectively cleave the lecticans. The purpose of these experiments was to determine whether the production of brevican or the ADAMTS-cleaved fragments of brevican were altered after deafferentation and reinnervation of the dentate gyrus via entorhinal cortex lesion (ECL. Results In the C57Bl6J mouse, synaptic density in the molecular layer of the dentate gyrus, as measured by synaptophysin levels in ELISA, was significantly attenuated 2 days (nearly 50% of contralateral and 7 days after lesion and returned to levels not different from the contralateral region at 30 days. Immunoreactive brevican in immunoblot was elevated 2 days after lesion, whereas there was a significant increase in the proteolytic product at 7, but not 30 days post-lesion. ADAMTS activity, estimated using the ratio of the specific ADAMTS-derived brevican fragment and intact brevican levels was increased at 7 days, but was not different from the contralateral side at 2 or 30 days after deafferentation. Conclusion These findings indicate that ADAMTS activity in the dentate outer molecular layer (OML is elevated during the initial synaptic reinnervation period (7 days after lesion. Therefore, proteolytic processing of brevican appears to be a significant extracellular event in the remodeling of the dentate after EC lesion, and may modulate the process of sprouting and

  3. Adenosine A2A receptors modulate the dopamine D2 receptor-mediated inhibition of synaptic transmission in the mouse prefrontal cortex.

    Science.gov (United States)

    Real, Joana I; Simões, Ana Patrícia; Cunha, Rodrigo A; Ferreira, Samira G; Rial, Daniel

    2018-05-01

    Prefrontal cortex (PFC) circuits are modulated by dopamine acting on D 1 - and D 2 -like receptors, which are pharmacologically exploited to manage neuropsychiatric conditions. Adenosine A 2A receptors (A 2 A R) also control PFC-related responses and A 2 A R antagonists are potential anti-psychotic drugs. As tight antagonistic A 2 A R-D 2 R and synergistic A 2 A R-D 1 R interactions occur in other brain regions, we now investigated the crosstalk between A 2 A R and D 1 /D 2 R controlling synaptic transmission between layers II/III and V in mouse PFC coronal slices. Dopamine decreased synaptic transmission, a presynaptic effect based on the parallel increase in paired-pulse responses. Dopamine inhibition was prevented by the D 2 R-like antagonist sulpiride but not by the D 1 R antagonist SCH23390 and was mimicked by the D 2 R agonist sumanirole, but not by the agonists of either D 4 R (A-412997) or D 3 R (PD128907). Dopamine inhibition was prevented by the A 2 A R antagonist, SCH58261, and attenuated in A 2 A R knockout mice. Accordingly, triple-labelling immunocytochemistry experiments revealed the co-localization of A 2 A R and D 2 R immunoreactivity in glutamatergic (vGluT1-positive) nerve terminals of the PFC. This reported positive A 2 A R-D 2 R interaction controlling PFC synaptic transmission provides a mechanistic justification for the anti-psychotic potential of A 2 A R antagonists. © 2018 Federation of European Neuroscience Societies and John Wiley & Sons Ltd.

  4. The functional upregulation of piriform cortex is associated with cross-modal plasticity in loss of whisker tactile inputs.

    Directory of Open Access Journals (Sweden)

    Bing Ye

    Full Text Available Cross-modal plasticity is characterized as the hypersensitivity of remaining modalities after a sensory function is lost in rodents, which ensures their awareness to environmental changes. Cellular and molecular mechanisms underlying cross-modal sensory plasticity remain unclear. We aim to study the role of different types of neurons in cross-modal plasticity.In addition to behavioral tasks in mice, whole-cell recordings at the excitatory and inhibitory neurons, and their two-photon imaging, were conducted in piriform cortex. We produced a mouse model of cross-modal sensory plasticity that olfactory function was upregulated by trimming whiskers to deprive their sensory inputs. In the meantime of olfactory hypersensitivity, pyramidal neurons and excitatory synapses were functionally upregulated, as well as GABAergic cells and inhibitory synapses were downregulated in piriform cortex from the mice of cross-modal sensory plasticity, compared with controls. A crosswire connection between barrel cortex and piriform cortex was established in cross-modal plasticity.An upregulation of pyramidal neurons and a downregulation of GABAergic neurons strengthen the activities of neuronal networks in piriform cortex, which may be responsible for olfactory hypersensitivity after a loss of whisker tactile input. This finding provides the clues for developing therapeutic strategies to promote sensory recovery and substitution.

  5. Downstream of tyrosine kinase/docking protein 6, as a novel substrate of tropomyosin-related kinase C receptor, is involved in neurotrophin 3-mediated neurite outgrowth in mouse cortex neurons

    Directory of Open Access Journals (Sweden)

    Yuan Jian

    2010-06-01

    Full Text Available Abstract Background The downstream of tyrosine kinase/docking protein (Dok adaptor protein family has seven members, Dok1 to Dok7, that act as substrates of multiple receptor tyrosine kinase and non-receptor tyrosine kinase. The tropomyosin-related kinase (Trk receptor family, which has three members (TrkA, TrkB and TrkC, are receptor tyrosine kinases that play pivotal roles in many stages of nervous system development, such as differentiation, migration, axon and dendrite projection and neuron patterning. Upon related neurotrophin growth factor stimulation, dimerisation and autophosphorylation of Trk receptors can occur, recruiting adaptor proteins to mediate signal transduction. Results In this report, by using yeast two-hybrid assays, glutathione S-transferase (GST precipitation assays and coimmunoprecipitation (Co-IP experiments, we demonstrate that Dok6 selectively binds to the NPQY motif of TrkC through its phosphotyrosine-binding (PTB domain in a kinase activity-dependent manner. We further confirmed their interaction by coimmunoprecipitation and colocalisation in E18.5 mouse cortex neurons, which provided more in vivo evidence. Next, we demonstrated that Dok6 is involved in neurite outgrowth in mouse cortex neurons via the RNAi method. Knockdown of Dok6 decreased neurite outgrowth in cortical neurons upon neurotrophin 3 (NT-3 stimulation. Conclusions We conclude that Dok6 interacts with the NPQY motif of the TrkC receptor through its PTB domain in a kinase activity-dependent manner, and works as a novel substrate of the TrkC receptor involved in NT-3-mediated neurite outgrowth in mouse cortex neurons.

  6. The CMS Barrel Muon trigger upgrade

    International Nuclear Information System (INIS)

    Triossi, A.; Sphicas, P.; Bellato, M.; Montecassiano, F.; Ventura, S.; Ruiz, J.M. Cela; Bedoya, C. Fernandez; Tobar, A. Navarro; Fernandez, I. Redondo; Ferrero, D. Redondo; Sastre, J.; Ero, J.; Wulz, C.; Flouris, G.; Foudas, C.; Loukas, N.; Mallios, S.; Paradas, E.; Guiducci, L.; Masetti, G.

    2017-01-01

    The increase of luminosity expected by LHC during Phase1 will impose tighter constraints for rate reduction in order to maintain high efficiency in the CMS Level1 trigger system. The TwinMux system is the early layer of the muon barrel region that concentrates the information from different subdetectors: Drift Tubes, Resistive Plate Chambers and Outer Hadron Calorimeter. It arranges the slow optical trigger links from the detector chambers into faster links (10 Gbps) that are sent in multiple copies to the track finders. Results from collision runs, that confirm the satisfactory operation of the trigger system up to the output of the barrel track finder, will be shown.

  7. Cerebral glucose utilization after vasopressin barrel rotation or bicuculline seizures

    International Nuclear Information System (INIS)

    Wurpel, J.; Dundore, R.; Bryan, R.; Keil, L.; Severs, W.B.

    1986-01-01

    Intraventricular (ivt) arginine vasopressin (AVP) causes a violent motor behavior termed barrel rotation (BR). AVP-BR is affected by visual/vestibular sensory input and may be related to other CNS motor disorders (seizures). Local cerebral glucose utilization (LCGU) was compared in SD rats during AVP-BR and bicuculline (BIC) seizures. Three groups were used: saline-ivt; AVP-ivt 0.5 μg; BIC-5.5 mg/kg,sc. 14 C-glucose (40 μCI iv) was injected 15 sec. after ivt-saline or AVP or onset of BIC seizures. Rats were decapitated 10 min. after 14 C-glucose. Brains were removed and dissected into 19 regions which were digested and glucose uptake quantified by liquid scintillation counting. LCGU was significantly increased in all CNS areas during BIC seizures vs controls (21-92%; p < 0.05 ANOVA). LCGU exhibits variable (upward arrow, downward arrow) changes in discrete areas during AVP-BR (p < .05). Glucose uptake increased in: cortex-olfactory (21%), sensory (9%), motor (8%) cerebellum-rt (13%) and 1t (17%) hemispheres, vermis (6%); pyramidal tract (6%); mesencephalon (5%); and pons (8%). Two areas decreased LCGU during AVP-BR: auditory cortex (-8%) and hippocampus (-11%). AVP-BR exhibits distinct changes in LCGU vs BIC seizures

  8. CMS : the first barrel ring completed !

    CERN Multimedia

    Laurent Guiraud

    2000-01-01

    On 14 November, the CMS collaboration and the German firm DWE celebrated the successful construction of the detector's first yoke barrel ring. To mark the occasion, those in charge of the construction at CERN and DWE posed for the camera in the middle of the giant component.

  9. Iron Blocks of CMS Magnet Barrel Yoke.

    CERN Multimedia

    2000-01-01

    On the occasion of presenting the CMS Award 2000 to Deggendorfer Werft und Eisenbau GmbH the delivered blocks were inspected at CERN Point 5. From left to right: H. Gerwig (CERN, CMS Magnet Barrel Yoke Coordinator), G. Waurick (CERN), F. Leher (DWE, Project Engineer) and W. Schuster (DWE, Project Manager).

  10. ATLAS semiconductor tracker installed into its barrel

    CERN Multimedia

    Maximilien Brice

    2005-01-01

    The ATLAS silicon tracker is installed in the silicon tracker barrel. Absolute precision was required in this operation to ensure that the tracker was inserted without damage through minimal clearance. The installation was performed in a clean room on the CERN site so that no impurities in the air would contaminate the tracker's systems.

  11. Building CMS Pixel Barrel Detectur Modules

    CERN Document Server

    König, S; Horisberger, R.; Meier, B.; Rohe, T.; Streuli, S.; Weber, R.; Kastli, H.Chr.; Erdmann, W.

    2007-01-01

    For the barrel part of the CMS pixel tracker about 800 silicon pixel detector modules are required. The modules are bump bonded, assembled and tested at the Paul Scherrer Institute. This article describes the experience acquired during the assembly of the first ~200 modules.

  12. Celebration for the ATLAS Barrel Toroid magnet

    CERN Multimedia

    2007-01-01

    Representatives from Funding Agencies and Barrel Toroid Magnet Laboratories during the ceremony. From left to right: Jean Zinn-Justin (Head of DAPNIA/CEA/Saclay), CERN Director-General Robert Aymar, and Roberto Petronzio (President INFN).Allan Clark (DPNC University Geneva) and Enrique Fernandez (IFAE Barcelona) were among the guests visiting the ATLAS cavern. The barrel toroid is visible in the background. A celebration took place at Point 1 on 13 December to toast the recent powering-up of the ATLAS barrel toroid magnet to full field (Bulletin No. 47-48/06). About 70 guests were invited to attend, mainly composed of representatives from funding partners and key members of the laboratory management teams of the barrel toroid magnet, representing CEA France, INFN Italy, BMBF Germany, Spain, Sweden, Switzerland, Russia, JINR Dubna and CERN. An introductory speech by ATLAS spokesperson Peter Jenni the scene for evening. This was followed by the ATLAS magnet system project leader Herman Ten Kate's account of the...

  13. Last Few Metres for the Barrel Calorimeter

    CERN Multimedia

    Nyman, T.

    On Friday 4th November, the ATLAS Barrel Calorimeter was moved from its assembly point at the side of the ATLAS cavern to the centre of the toroidal magnet system. The detector was finally aligned, to the precision of within a millimetre, on Wednesday 9th November. The ATLAS installation team, led by Tommi Nyman, after having positioned the Barrel Calorimeter in its final location in the ATLAS experimental cavern UX15. The Barrel Calorimeter which will absorb and measure the energy of photons, electrons and hadrons at the core of the ATLAS detector is 8.6 meters in diameter, 6.8 meters long, and weighs over 1600 Tonnes. It consists of two concentric cylindrical detector elements. The innermost comprises aluminium pressure vessels containing the liquid argon electromagnetic calorimeter and the solenoid magnet. The outermost is an assembly of 64 hadron tile calorimeter sectors. Assembled 18 meters away from its final position, the Barrel Calorimeter was relocated with the help of a railway, which allows ...

  14. ATLAS Barrel Toroid magnet reached nominal field

    CERN Multimedia

    2006-01-01

     On 9 November the barrel toroid magnet reached its nominal field of 4 teslas, with an electrical current of 21 000 amperes (21 kA) passing through the eight superconducting coils as shown on this graph

  15. Connexin31.1 deficiency in the mouse impairs object memory and modulates open-field exploration, acetylcholine esterase levels in the striatum, and cAMP response element-binding protein levels in the striatum and piriform cortex.

    Science.gov (United States)

    Dere, E; Zheng-Fischhöfer, Q; Viggiano, D; Gironi Carnevale, U A; Ruocco, L A; Zlomuzica, A; Schnichels, M; Willecke, K; Huston, J P; Sadile, A G

    2008-05-02

    Neuronal gap junctions in the brain, providing intercellular electrotonic signal transfer, have been implicated in physiological and behavioral correlates of learning and memory. In connexin31.1 (Cx31.1) knockout (KO) mice the coding region of the Cx31.1 gene was replaced by a LacZ reporter gene. We investigated the impact of Cx31.1 deficiency on open-field exploration, the behavioral response to an odor, non-selective attention, learning and memory performance, and the levels of memory-related proteins in the hippocampus, striatum and the piriform cortex. In terms of behavior, the deletion of the Cx31.1 coding DNA in the mouse led to increased exploratory behaviors in a novel environment, and impaired one-trial object recognition at all delays tested. Despite strong Cx31.1 expression in the peripheral and central olfactory system, Cx31.1 KO mice exhibited normal behavioral responses to an odor. We found increased levels of acetylcholine esterase (AChE) and cAMP response element-binding protein (CREB) in the striatum of Cx31.1 KO mice. In the piriform cortex the Cx31.1 KO mice had an increased heterogeneity of CREB expression among neurons. In conclusion, gap-junctions featuring the Cx31.1 protein might be involved in open-field exploration as well as object memory and modulate levels of AChE and CREB in the striatum and piriform cortex.

  16. Barrel calorimeter of the CMD-3 detector

    Energy Technology Data Exchange (ETDEWEB)

    Shebalin, V. E., E-mail: V.E.Shebalin@inp.nsk.su; Anisenkov, A. V.; Aulchenko, V. M.; Bashtovoy, N. S. [Russian Academy of Sciences, Budker Institute of Nuclear Physics, Siberian Branch (Russian Federation); Epifanov, D. A. [University of Tokyo, Department of Physics (Japan); Epshteyn, L. B.; Grebenuk, A. A.; Ignatov, F. V.; Erofeev, A. L.; Kovalenko, O. A.; Kozyrev, A. N.; Kuzmin, A. S.; Logashenko, I. B.; Mikhailov, K. Yu.; Razuvaev, G. P.; Ruban, A. A.; Shwartz, B. A.; Talyshev, A. A.; Titov, V. M.; Yudin, Yu. V. [Russian Academy of Sciences, Budker Institute of Nuclear Physics, Siberian Branch (Russian Federation)

    2015-12-15

    The structure of the barrel calorimeter of the CMD-3 detector is presented in this work. The procedure of energy calibration of the calorimeter and the method of photon energy restoration are described. The distinctive feature of this barrel calorimeter is its combined structure; it is composed of two coaxial subsystems: a liquid xenon calorimeter and a crystalline CsI calorimeter. The calorimeter spatial resolution of the photon conversion point is about 2 mm, which corresponds to an angular resolution of ∼6 mrad. The energy resolution of the calorimeter is about 8% for photons with energy of 200 MeV and 4% for photons with energy of 1 GeV.

  17. Barrel calorimeter of the CMD-3 detector

    International Nuclear Information System (INIS)

    Shebalin, V. E.; Anisenkov, A. V.; Aulchenko, V. M.; Bashtovoy, N. S.; Epifanov, D. A.; Epshteyn, L. B.; Grebenuk, A. A.; Ignatov, F. V.; Erofeev, A. L.; Kovalenko, O. A.; Kozyrev, A. N.; Kuzmin, A. S.; Logashenko, I. B.; Mikhailov, K. Yu.; Razuvaev, G. P.; Ruban, A. A.; Shwartz, B. A.; Talyshev, A. A.; Titov, V. M.; Yudin, Yu. V.

    2015-01-01

    The structure of the barrel calorimeter of the CMD-3 detector is presented in this work. The procedure of energy calibration of the calorimeter and the method of photon energy restoration are described. The distinctive feature of this barrel calorimeter is its combined structure; it is composed of two coaxial subsystems: a liquid xenon calorimeter and a crystalline CsI calorimeter. The calorimeter spatial resolution of the photon conversion point is about 2 mm, which corresponds to an angular resolution of ∼6 mrad. The energy resolution of the calorimeter is about 8% for photons with energy of 200 MeV and 4% for photons with energy of 1 GeV

  18. Associations of unilateral whisker and olfactory signals induce synapse formation and memory cell recruitment in bilateral barrel cortices: cellular mechanism for unilateral training toward bilateral memory

    Directory of Open Access Journals (Sweden)

    Zilong Gao

    2016-12-01

    Full Text Available Somatosensory signals and operative skills learned by unilateral limbs can be retrieved bilaterally. In terms of cellular mechanism underlying this unilateral learning toward bilateral memory, we hypothesized that associative memory cells in bilateral cortices and synapse innervations between them were produced. In the examination of this hypothesis, we have observed that paired unilateral whisker and odor stimulations led to odorant-induced whisker motions in bilateral sides, which were attenuated by inhibiting the activity of barrel cortices. In the mice that showed bilateral cross-modal responses, the neurons in both sides of barrel cortices became to encode this new odor signal alongside the innate whisker signal. Axon projections and synapse formations from the barrel cortex, which was co-activated with the piriform cortex, toward its contralateral barrel cortex were upregulated. Glutamatergic synaptic transmission in bilateral barrel cortices was upregulated and GABAergic synaptic transmission was downregulated. The associative activations of the sensory cortices facilitate new axon projection, glutamatergic synapse formation and GABAergic synapse downregulation, which drive the neurons to be recruited as associative memory cells in the bilateral cortices. Our data reveals the productions of associative memory cells and synapse innervations in bilateral sensory cortices for unilateral training toward bilateral memory.

  19. Taxonomy Icon Data: barrel medic [Taxonomy Icon

    Lifescience Database Archive (English)

    Full Text Available barrel medic Medicago truncatula Medicago_truncatula_L.png Medicago_truncatula_NL.png Medi...cago_truncatula_S.png Medicago_truncatula_NS.png http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Medi...cago+truncatula&t=L http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Medicago+truncatula&t=NL http://biosci...encedbc.jp/taxonomy_icon/icon.cgi?i=Medicago+truncatula&t=S http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Medicago+truncatula&t=NS ...

  20. First half of CMS inner tracker barrel

    CERN Multimedia

    Maximilien Brice

    2006-01-01

    The first half of the CMS inner tracker barrel is seen in this image consisting of three layers of silicon modules which will be placed at the centre of the CMS experiment at the LHC in CERN. Laying close to the interaction point of the 14 TeV proton-proton collisions, the silicon used here must be able to survive high doses of radiation and a 4 T magnetic field without damage.

  1. Alignment of the MSGC barrel support structure

    International Nuclear Information System (INIS)

    Kari, Tammi; Miikka, Kotamaki; Tommi, Vanhala; Antti, Onnela

    1999-01-01

    The MSGC barrel is a sub-part of the tracking system of the CMS experiment at the LHC. The mechanical support structure of the MSGC barrel consists of ladder-like support beams carrying the detector modules and of four disks supporting the ladders. The required alignment precision of the modules, a few tens of micrometers, is designed to be obtained by precise part manufacture and by careful measurement of the alignment during the assembly of the structure. In the paper the use of digital photogrammetry for the measurement of the alignment of the disks and for the structural verification is presented. Digital photogrammetry was chosen from a number of potential methods after a careful evaluation. The use of photogrammetry for the structural verification of a prototype is presented. The displacements were measured both of unloaded and loaded disk by using photogrammetry and linear displacement transducers for verification. The displacements obtained from the two measurement methods corresponded well, not only to each other, but also to the results given by finite element analysis. The structural verification will be done and the alignment procedure will be tested with a full-sized prototype of a half of the MSGC barrel. Preparations for the photogrammetry measurements are presented and the design of the required supplementary equipment is shown. (authors)

  2. Alignment of the MSGC barrel support structure

    Energy Technology Data Exchange (ETDEWEB)

    Kari, Tammi; Miikka, Kotamaki; Tommi, Vanhala [HIP, Helsinki Institute of Physics, CERN/EP, Geneva (Switzerland); Antti, Onnela [CERN, Conseil Europeen pour la recherche nucleaire, Laboratoire europeen pour la physique des particules, Geneve (Switzerland)

    1999-07-01

    The MSGC barrel is a sub-part of the tracking system of the CMS experiment at the LHC. The mechanical support structure of the MSGC barrel consists of ladder-like support beams carrying the detector modules and of four disks supporting the ladders. The required alignment precision of the modules, a few tens of micrometers, is designed to be obtained by precise part manufacture and by careful measurement of the alignment during the assembly of the structure. In the paper the use of digital photogrammetry for the measurement of the alignment of the disks and for the structural verification is presented. Digital photogrammetry was chosen from a number of potential methods after a careful evaluation. The use of photogrammetry for the structural verification of a prototype is presented. The displacements were measured both of unloaded and loaded disk by using photogrammetry and linear displacement transducers for verification. The displacements obtained from the two measurement methods corresponded well, not only to each other, but also to the results given by finite element analysis. The structural verification will be done and the alignment procedure will be tested with a full-sized prototype of a half of the MSGC barrel. Preparations for the photogrammetry measurements are presented and the design of the required supplementary equipment is shown. (authors)

  3. a7 nicotinic receptor agonism mitigates phencyclidine-induced changes in synaptophysin and Arc gene expression in the mouse prefrontal cortex

    DEFF Research Database (Denmark)

    Thomsen, Morten S; Hansen, Henrik H; Mikkelsen, Jens D

    2010-01-01

    Repeated phencyclidine (PCP) administration in mice reproduces several histopathological features of schizophrenia, such as reduced synaptophysin and parvalbumin mRNA expression in the frontal cortex. These changes can be prevented by co-administering the a7 nicotinic acetylcholine receptor (n...

  4. α7 nicotinic receptor agonism mitigates phencyclidine-induced changes in synaptophysin and Arc gene expression in the mouse prefrontal cortex

    DEFF Research Database (Denmark)

    Thomsen, Morten S; Hansen, Henrik H; Mikkelsen, Jens D

    2010-01-01

    Repeated phencyclidine (PCP) administration in mice reproduces several histopathological features of schizophrenia, such as reduced synaptophysin and parvalbumin mRNA expression in the frontal cortex. These changes can be prevented by co-administering the α7 nicotinic acetylcholine receptor (n...

  5. Robotic mounting of ATLAS barrel SCT modules

    International Nuclear Information System (INIS)

    Nickerson, R.B.; Viehhauser, G.; Wastie, R.; Terada, S.; Unno, Y.; Kohriki, T.; Ikegami, Y.; Hara, K.; Kobayashi, H.; Barbier, G.; Clark, A.G.; Perrin, E.; Carter, A.A.; Mistry, J.; Morris, J.

    2006-01-01

    The 2112 silicon detector modules of the barrel part of the ATLAS SemiConductor Tracker (SCT) have been mounted on their carbon fibre support structure. Module insertion, placement and fixing were performed by robotic assembly tooling. We report on our experience with this assembly method. Part of the mounting sequence involves a partial survey of elements of the support structure which is needed to align the modules properly during insertion. An analysis of these data is used to estimate the positional accuracy of the robots

  6. 3D Printing the ATLAS' barrel toroid

    CERN Document Server

    Goncalves, Tiago Barreiro

    2016-01-01

    The present report summarizes my work as part of the Summer Student Programme 2016 in the CERN IR-ECO-TSP department (International Relations – Education, Communication & Outreach – Teacher and Student Programmes). Particularly, I worked closely with the S’Cool LAB team on a science education project. This project included the 3D designing, 3D printing, and assembling of a model of the ATLAS’ barrel toroid. A detailed description of the project' development is presented and a short manual on how to use 3D printing software and hardware is attached.

  7. Failure analysis of a barrel exposed to high temperature

    International Nuclear Information System (INIS)

    Usman, A.; Salam, I.; Rizvi, S.A.; Qasir, S.

    2005-01-01

    The paper deals with the study of a tank gun barrel which had failed after firing only a few rounds. The failure was in the form of bulging at the muzzle end (ME). The material of the barrel was characterized using different techniques including chemical and mechanical testing, optical microscopy and electron microscopy. Study disclosed that the barrel was subjected to excessively high temperature that resulted in its softening and consequent bulging under high pressure of the round. (author)

  8. Progress of the EM Barrel Presampler Assembly

    CERN Multimedia

    Hostachy, J.Y.

    The liquid argon barrel presampler is a separate detector which will be placed in front of the electromagnetic barrel calorimeter, in the same cryostat. It is made of 32×2 sectors, each of them being 3.1 m long, about 28 cm large and a few cm thick. Three countries are involved in its construction: France (ISN-Grenoble), Sweden (KTH-Stockholm) and Morocco (Universities: Hassan II Ain Chock-Casablanca and Mohamed V-Rabat, and CNESTEN-Rabat). The design of the presampler started ten years ago and the series production began at the end of the year 2000. Today two-thirds of the sectors are produced and validated. In November 2002, half the detector (i.e. 32 sectors), was inserted on the internal face of the first EM calorimeter wheel (see pictures). Despite the fact that only 0.4 mm was available between sectors, it was possible to insert them all without meeting major difficulties. This operation was led by a team of four people, the sectors being systematically tested after insertion in the wheel. The inserti...

  9. ATLAS TRT Barrel in Test Beam

    CERN Multimedia

    Luehring, F

    In July, the TRT group made a highly successful test of 6 Barrel TRT modules in the ATLAS H8 testbeam. Over 3000 TRT straw tubes (4 mm diameter gas drift tubes) were instrumented and found to operate well. The prototype represents 1/16 of the ATLAS TRT barrel and was assembled from TRT modules produced as spares. This was the largest scale test of the TRT to this date and the measured detector performance was as good as or better than what was expected in all cases. The 2004 TRT testbeam setup before final cabling was attached. The readout chain and central DAQ system used in the TRT testbeam is a final prototype for the ATLAS experiment. The TRT electronics used to read out the data were: The Amplifier/Shaper/Discriminator with Baseline Restoration (ASDBLR) chip is the front-end analog chip that shapes and discriminates the electronic pulses generated by the TRT straws. The Digital Time Measurement Read Out Chip (DTMROC) measures the time of the pulse relative to the beam crossing time. The TRT-ROD ...

  10. Cosmic Ray Data in TRT Barrel

    CERN Multimedia

    M. Hance

    "I had a great day in August when I went into SR1," said Daniel Froidevaux, former project leader of the ATLAS Transition Radiation Tracker, "not only had all SCT barrels arrived at CERN, but there were cosmic ray tracks seen in the TRT!" Daniel's excitement was mirrored by the rest of the TRT collaboration when, on July 29, the first cosmic ray tracks were seen in the barrel. Along with many others in the community, Daniel was quick to point out that this is the cumulative result of years of R&D, test beam work, and an intense installation and integration schedule. Indeed, the cosmic ray readout is only possible through the coordination of many efforts, from detector mechanics to module assembly, power and high voltage control, cooling, gas systems, electronics and cabling, data acquisition, and monitoring. "Many people have worked very hard on the the TRT, some of them for more than 10 years," said Brig Williams, the leader of the UPenn group responsible for much of the TRT front end electronics. He ...

  11. The CMS Barrel Muon Trigger Upgrade

    CERN Document Server

    Triossi, Andrea

    2017-01-01

    ABSTRACT: The increase of luminosity expected by LHC during Phase 1 will impose several constrains for rate reduction while maintaining high efficiency in the CMS Level 1 trigger system. The TwinMux system is the early layer of the muon barrel region that concentrates the information from different subdetectors DT, RPC and HO. It arranges and fan-out the slow optical trigger links from the detector chambers into faster links (10 Gbps) that are sent to the track finders. Results, from collision runs, that confirm the satisfactory operation of the trigger system up to the output of the barrel track finder, will be shown. SUMMARY: In view of the increase of luminosity during phase 1 upgrade of LHC, the muon trigger chain of the Compact Muon Solenoid (CMS) experiment underwent considerable improvements. The muon detector was designed for preserving the complementarity and redundancy of three separate muon detection systems, Cathode Strip Chambers (CSC), Drift Tubes (DT) and Resistive Plate Chambers (RPC), until ...

  12. Strain differences in basal and post-citalopram extracellular 5-HT in the mouse medial prefrontal cortex and dorsal hippocampus: relation with tryptophan hydroxylase-2 activity.

    Science.gov (United States)

    Calcagno, E; Canetta, A; Guzzetti, S; Cervo, L; Invernizzi, R W

    2007-11-01

    We used the microdialysis technique to compare basal extracellular serotonin (5-HT) and the response to citalopram in different strains of mice with functionally different allelic forms of tryptophan hydroxylase-2 (TPH-2), the rate-limiting enzyme in brain 5-HT synthesis. DBA/2J, DBA/2N and BALB/c mice carrying the 1473G allele of TPH-2 had less dialysate 5-HT in the medial prefrontal cortex and dorsal hippocampus (DH) (20-40% reduction) than C57BL/6J and C57BL/6N mice carrying the 1473C allele. Extracellular 5-HT estimated by the zero-net flux method confirmed the result of conventional microdialysis. Citalopram, 1.25, 5 and 20 mg/kg, dose-dependently raised extracellular 5-HT in the medial prefrontal cortex of C57BL/6J mice, with maximum effect at 5 mg/kg, but had significantly less effect in DBA/2J and BALB/c mice and in the DH of DBA/2J mice. A tryptophan (TRP) load enhanced basal extracellular 5-HT in the medial prefrontal cortex of DBA/2J mice but did not affect citalopram's ability to raise cortical and hippocampal extracellular 5-HT. The impairment of 5-HT synthesis quite likely accounts for the reduction of basal 5-HT and the citalopram-induced rise in mice carrying the mutated enzyme. These findings might explain why DBA/2 and BALB/c mice do not respond to citalopram in the forced swimming test. Although TRP could be a useful strategy to improve the antidepressant effect of citalopram (Cervo et al. 2005), particularly in subjects with low 5-HT synthesis, the contribution of serotonergic and non-serotonergic mechanisms to TRP's effect remains to be elucidated.

  13. The Functioning of a Cortex without Layers

    Directory of Open Access Journals (Sweden)

    Julien Guy

    2017-07-01

    Full Text Available A major hallmark of cortical organization is the existence of a variable number of layers, i.e., sheets of neurons stacked on top of each other, in which neurons have certain commonalities. However, even for the neocortex, variable numbers of layers have been described and it is just a convention to distinguish six layers from each other. Whether cortical layers are a structural epiphenomenon caused by developmental dynamics or represent a functionally important modularization of cortical computation is still unknown. Here we present our insights from the reeler mutant mouse, a model for a developmental, “molecular lesion”-induced loss of cortical layering that could serve as ground truth of what an intact layering adds to the cortex in terms of functionality. We could demonstrate that the reeler neocortex shows no inversion of cortical layers but rather a severe disorganization that in the primary somatosensory cortex leads to the complete loss of layers. Nevertheless, the somatosensory system is well organized. When exploring an enriched environment with specific sets of whiskers, activity-dependent gene expression takes place in the corresponding modules. Precise whisker stimuli lead to the functional activation of somatotopically organized barrel columns as visualized by intrinsic signal optical imaging. Similar results were obtained in the reeler visual system. When analyzing pathways that could be responsible for preservation of tactile perception, lemniscal thalamic projections were found to be largely intact, despite the smearing of target neurons across the cortical mantle. However, with optogenetic experiments we found evidence for a mild dispersion of thalamic synapse targeting on layer IV-spiny stellate cells, together with a general weakening in thalamocortical input strength. This weakening of thalamic inputs was compensated by intracortical mechanisms involving increased recurrent excitation and/or reduced feedforward

  14. Neuroanatomical characterization of the cellular and axonal architecture of subcortical band heterotopia in the BXD29-Tlr4lps-2J/J mouse cortex.

    Science.gov (United States)

    Ramos, Raddy L; Toia, Alyssa R; Pasternack, Daniel M; Dotzler, Timothy P; Cuoco, Joshua A; Esposito, Anthony W; Le, Megan M; Parker, Alexander K; Goodman, Jeffrey H; Sarkisian, Matthew R

    2016-11-19

    Subcortical band heterotopia (SBH) are malformations of the human cerebral cortex typically associated with epilepsy and cognitive delay/disability. Rodent models of SBH have demonstrated strong face validity as they are accompanied by both cognitive deficits and spontaneous seizures or reduced seizure threshold. BXD29-Tlr4 lps-2J /J recombinant inbred mice display striking bilateral SBH, partial callosal agenesis, morphological changes in subcortical structures of the auditory pathway, and display sensory deficits in behavioral tests (Rosen et al., 2013; Truong et al., 2013, 2015). Surprisingly, these mice show no cognitive deficits and have a higher seizure threshold to chemi-convulsive treatment (Gabel et al., 2013) making them different than other rodent SBH models described previously. In the present report, we perform a detailed characterization of the cellular and axonal constituents of SBH in BXD29-Tlr4 lps-2J /J mice and demonstrate that various types of interneurons and glia as well as cortical and subcortical projections are found in SBH. In addition, the length of neuronal cilia was reduced in SBH compared to neurons in the overlying and adjacent normotopic cortex. Finally, we describe additional and novel malformations of the hippocampus and neocortex present in BXD29-Tlr4 lps-2J /J mice. Together, our findings in BXD29-Tlr4 lps-2J /J mice are discussed in the context of the known neuroanatomy and phenotype of other SBH rodent models. Copyright © 2016 IBRO. Published by Elsevier Ltd. All rights reserved.

  15. Social Isolation During the Critical Period Reduces Synaptic and Intrinsic Excitability of a Subtype of Pyramidal Cell in Mouse Prefrontal Cortex.

    Science.gov (United States)

    Yamamuro, Kazuhiko; Yoshino, Hiroki; Ogawa, Yoichi; Makinodan, Manabu; Toritsuka, Michihiro; Yamashita, Masayuki; Corfas, Gabriel; Kishimoto, Toshifumi

    2018-03-01

    Juvenile social experience is crucial for the functional development of forebrain regions, especially the prefrontal cortex (PFC). We previously reported that social isolation for 2 weeks after weaning induces prefrontal cortex dysfunction and hypomyelination. However, the effect of social isolation on physiological properties of PFC neuronal circuit remained unknown. Since hypomyelination due to isolation is prominent in deep-layer of medial PFC (mPFC), we focused on 2 types of Layer-5 pyramidal cells in the mPFC: prominent h-current (PH) cells and nonprominent h-current (non-PH) cells. We found that a 2-week social isolation after weaning leads to a specific deterioration in action potential properties and reduction in excitatory synaptic inputs in PH cells. The effects of social isolation on PH cells, which involve reduction in functional glutamatergic synapses and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid/N-methyl-d-aspartate charge ratio, are specific to the 2 weeks after weaning and to the mPFC. We conclude that juvenile social experience plays crucial roles in the functional development in a subtype of Layer-5 pyramidal cells in the mPFC. Since these neurons project to subcortical structures, a deficit in social experience during the critical period may result in immature neural circuitry between mPFC and subcortical targets. © The Author 2017. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  16. Activation of the mouse primary visual cortex by medial prefrontal subregion stimulation is not mediated by cholinergic basalo-cortical projections

    Directory of Open Access Journals (Sweden)

    Hoang Nam eNguyen

    2015-02-01

    Full Text Available The medial prefrontal cortex (mPFC exerts top-down control of primary visual cortex (V1 activity. As there is no direct neuronal projection from mPFC to V1, this functional connection may use an indirect route, i.e., via basalo-cortical cholinergic projections. The cholinergic projections to V1 originate from neurons in the horizontal limb of the diagonal band of Broca (HDB, which receive neuronal projections from the ventral part of the mPFC, composed of prelimbic (PrL and infralimbic cortices (IL. Therefore, the objective of this study was to determine whether electrical stimulation of mice mPFC subregions activate 1 V1 neurons and 2 HDB cholinergic neurons, suggesting that the HDB serves as a relay point in the mPFC-V1 interaction. Neuronal activation was quantified using c-Fos immunocytochemistry or thallium autometallography for each V1 layer using automated particle analysis tools and optical density measurement. Stimulation of IL and PrL induced significantly higher c-Fos expression or thallium labelling in layers II/III and V of V1 in the stimulated hemisphere only. A HDB cholinergic neuron-specific lesion by saporin administration reduced IL-induced c-Fos expression in layers II/III of V1 but not in layer V. However, there was no c-Fos expression or thallium labelling in the HDB neurons, suggesting that this area was not activated by IL stimulation. Stimulation of another mPFC subarea, the anterior cingulate cortex (AC, which is involved in attention and receives input from V1, activated neither V1 nor HDB. The present results indicate that IL and PrL, but not AC, stimulation activates V1 with the minor involvement of the HDB cholinergic projections. These results suggest a functional link between the ventral mPFC and V1, but this function is only marginally supported by HDB cholinergic neurons and may involve other brain regions.

  17. 27 CFR 25.141 - Barrels and kegs.

    Science.gov (United States)

    2010-04-01

    ... OF THE TREASURY LIQUORS BEER Marks, Brands, and Labels § 25.141 Barrels and kegs. (a) General... shown on the bung or on the tap cover, or on a label securely affixed to each barrel or keg, the place... production: (i) May be shown as the only location on the bung, or on the tap cover, or on a separate label...

  18. Scraping the bottom of the barrel

    Energy Technology Data Exchange (ETDEWEB)

    Leite, L.F. [PETROBRAS (Brazil)

    2001-03-01

    This article focuses on technologies for upgrading residual streams to improve refiners margins, and reports on the refining technology programme (PROTER) set up by the Brazilian PETROBRAS company. Details are given of fluid catalytic cracking (FCC) pilot units at PETROBRAS's CENPES Research and Development Centre in Rio de Janeiro State, the development of new proprietary closed cyclone technology, the Ultramist feedstock injection device, the feed nozzle, and the high accessibility catalyst. FCC units at PETROBRAS, FCC ongoing projects, and the use of delayed coking to convert low value residues to high value residues are described along with other bottom of barrel projects such as residue hydrocracking, hydropyrolysis, and the production of a stable fuel emulsion from an asphalt residue stream.

  19. Sleep loss reduces the DNA-binding of BMAL1, CLOCK, and NPAS2 to specific clock genes in the mouse cerebral cortex.

    Directory of Open Access Journals (Sweden)

    Valérie Mongrain

    Full Text Available We have previously demonstrated that clock genes contribute to the homeostatic aspect of sleep regulation. Indeed, mutations in some clock genes modify the markers of sleep homeostasis and an increase in homeostatic sleep drive alters clock gene expression in the forebrain. Here, we investigate a possible mechanism by which sleep deprivation (SD could alter clock gene expression by quantifying DNA-binding of the core-clock transcription factors CLOCK, NPAS2, and BMAL1 to the cis-regulatory sequences of target clock genes in mice. Using chromatin immunoprecipitation (ChIP, we first showed that, as reported for the liver, DNA-binding of CLOCK and BMAL1 to target clock genes changes in function of time-of-day in the cerebral cortex. Tissue extracts were collected at ZT0 (light onset, -6, -12, and -18, and DNA enrichment of E-box or E'-box containing sequences was measured by qPCR. CLOCK and BMAL1 binding to Cry1, Dbp, Per1, and Per2 depended on time-of-day, with maximum values reached at around ZT6. We then observed that SD, performed between ZT0 and -6, significantly decreased DNA-binding of CLOCK and BMAL1 to Dbp, consistent with the observed decrease in Dbp mRNA levels after SD. The DNA-binding of NPAS2 and BMAL1 to Per2 was also decreased by SD, although SD is known to increase Per2 expression in the cortex. DNA-binding to Per1 and Cry1 was not affected by SD. Our results show that the sleep-wake history can affect the clock molecular machinery directly at the level of chromatin binding thereby altering the cortical expression of Dbp and Per2 and likely other targets. Although the precise dynamics of the relationship between DNA-binding and mRNA expression, especially for Per2, remains elusive, the results also suggest that part of the reported circadian changes in DNA-binding of core clock components in tissues peripheral to the suprachiasmatic nuclei could, in fact, be sleep-wake driven.

  20. Sleep loss reduces the DNA-binding of BMAL1, CLOCK, and NPAS2 to specific clock genes in the mouse cerebral cortex.

    Science.gov (United States)

    Mongrain, Valérie; La Spada, Francesco; Curie, Thomas; Franken, Paul

    2011-01-01

    We have previously demonstrated that clock genes contribute to the homeostatic aspect of sleep regulation. Indeed, mutations in some clock genes modify the markers of sleep homeostasis and an increase in homeostatic sleep drive alters clock gene expression in the forebrain. Here, we investigate a possible mechanism by which sleep deprivation (SD) could alter clock gene expression by quantifying DNA-binding of the core-clock transcription factors CLOCK, NPAS2, and BMAL1 to the cis-regulatory sequences of target clock genes in mice. Using chromatin immunoprecipitation (ChIP), we first showed that, as reported for the liver, DNA-binding of CLOCK and BMAL1 to target clock genes changes in function of time-of-day in the cerebral cortex. Tissue extracts were collected at ZT0 (light onset), -6, -12, and -18, and DNA enrichment of E-box or E'-box containing sequences was measured by qPCR. CLOCK and BMAL1 binding to Cry1, Dbp, Per1, and Per2 depended on time-of-day, with maximum values reached at around ZT6. We then observed that SD, performed between ZT0 and -6, significantly decreased DNA-binding of CLOCK and BMAL1 to Dbp, consistent with the observed decrease in Dbp mRNA levels after SD. The DNA-binding of NPAS2 and BMAL1 to Per2 was also decreased by SD, although SD is known to increase Per2 expression in the cortex. DNA-binding to Per1 and Cry1 was not affected by SD. Our results show that the sleep-wake history can affect the clock molecular machinery directly at the level of chromatin binding thereby altering the cortical expression of Dbp and Per2 and likely other targets. Although the precise dynamics of the relationship between DNA-binding and mRNA expression, especially for Per2, remains elusive, the results also suggest that part of the reported circadian changes in DNA-binding of core clock components in tissues peripheral to the suprachiasmatic nuclei could, in fact, be sleep-wake driven.

  1. Age-specific function of α5β1 integrin in microglial migration during early colonization of the developing mouse cortex.

    Science.gov (United States)

    Smolders, Sophie Marie-Thérèse; Swinnen, Nina; Kessels, Sofie; Arnauts, Kaline; Smolders, Silke; Le Bras, Barbara; Rigo, Jean-Michel; Legendre, Pascal; Brône, Bert

    2017-07-01

    Microglia, the immune cells of the central nervous system, take part in brain development and homeostasis. They derive from primitive myeloid progenitors that originate in the yolk sac and colonize the brain mainly through intensive migration. During development, microglial migration speed declines which suggests that their interaction with the microenvironment changes. However, the matrix-cell interactions allowing dispersion within the parenchyma are unknown. Therefore, we aimed to better characterize the migration behavior and to assess the role of matrix-integrin interactions during microglial migration in the embryonic brain ex vivo. We focused on microglia-fibronectin interactions mediated through the fibronectin receptor α5β1 integrin because in vitro work indirectly suggested a role for this ligand-receptor pair. Using 2-photon time-lapse microscopy on acute ex vivo embryonic brain slices, we found that migration occurs in a saltatory pattern and is developmentally regulated. Most importantly, there is an age-specific function of the α5β1 integrin during microglial cortex colonization. At embryonic day (E) 13.5, α5β1 facilitates migration while from E15.5, it inhibits migration. These results indicate a developmentally regulated function of α5β1 integrin in microglial migration during colonization of the embryonic brain. © 2017 Wiley Periodicals, Inc.

  2. Loss of γ-tubulin, GCP-WD/NEDD1 and CDK5RAP2 from the Centrosome of Neurons in Developing Mouse Cerebral and Cerebellar Cortex

    International Nuclear Information System (INIS)

    Yonezawa, Satoshi; Shigematsu, Momoko; Hirata, Kazuto; Hayashi, Kensuke

    2015-01-01

    It has been recently reported that the centrosome of neurons does not have microtubule nucleating activity. Microtubule nucleation requires γ-tubulin as well as its recruiting proteins, GCP-WD/NEDD1 and CDK5RAP2 that anchor γ-tubulin to the centrosome. Change in the localization of these proteins during in vivo development of brain, however, has not been well examined. In this study we investigate the localization of γ-tubulin, GCP-WD and CDK5RAP2 in developing cerebral and cerebellar cortex with immunofluorescence. We found that γ-tubulin and its recruiting proteins were localized at centrosomes of immature neurons, while they were lost at centrosomes in mature neurons. This indicated that the loss of microtubule nucleating activity at the centrosome of neurons is due to the loss of γ-tubulin-recruiting proteins from the centrosome. RT-PCR analysis revealed that these proteins are still expressed after birth, suggesting that they have a role in microtubule generation in cell body and dendrites of mature neurons. Microtubule regrowth experiments on cultured mature neurons showed that microtubules are nucleated not at the centrosome but within dendrites. These data indicated the translocation of microtubule-organizing activity from the centrosome to dendrites during maturation of neurons, which would explain the mixed polarity of microtubules in dendrites

  3. NMDA receptors in mouse anterior piriform cortex initialize early odor preference learning and L-type calcium channels engage for long-term memory.

    Science.gov (United States)

    Mukherjee, Bandhan; Yuan, Qi

    2016-10-14

    The interactions of L-type calcium channels (LTCCs) and NMDA receptors (NMDARs) in memories are poorly understood. Here we investigated the specific roles of anterior piriform cortex (aPC) LTCCs and NMDARs in early odor preference memory in mice. Using calcium imaging in aPC slices, LTCC activation was shown to be dependent on NMDAR activation. Either D-APV (NMDAR antagonist) or nifedipine (LTCC antagonist) reduced somatic calcium transients in pyramidal cells evoked by lateral olfactory tract stimulation. However, nifedipine did not further reduce calcium in the presence of D-APV. In mice that underwent early odor preference training, blocking NMDARs in the aPC prevented short-term (3 hr) and long-term (24 hr) odor preference memory, and both memories were rescued when BayK-8644 (LTCC agonist) was co-infused. However, activating LTCCs in the absence of NMDARs resulted in loss of discrimination between the conditioned odor and a similar odor mixture at 3 hr. Elevated synaptic AMPAR expression at 3 hr was prevented by D-APV infusion but restored when LTCCs were directly activated, mirroring the behavioral outcomes. Blocking LTCCs prevented 24 hr memory and spared 3 hr memory. These results suggest that NMDARs mediate stimulus-specific encoding of odor memory while LTCCs mediate intracellular signaling leading to long-term memory.

  4. Globally intertwined evolutionary history of giant barrel sponges

    Science.gov (United States)

    Swierts, Thomas; Peijnenburg, Katja T. C. A.; de Leeuw, Christiaan A.; Breeuwer, Johannes A. J.; Cleary, Daniel F. R.; de Voogd, Nicole J.

    2017-09-01

    Three species of giant barrel sponge are currently recognized in two distinct geographic regions, the tropical Atlantic and the Indo-Pacific. In this study, we used molecular techniques to study populations of giant barrel sponges across the globe and assessed whether the genetic structure of these populations agreed with current taxonomic consensus or, in contrast, whether there was evidence of cryptic species. Using molecular data, we assessed whether giant barrel sponges in each oceanic realm represented separate monophyletic lineages. Giant barrel sponges from 17 coral reef systems across the globe were sequenced for mitochondrial (partial CO1 and ATP6 genes) and nuclear (ATPsβ intron) DNA markers. In total, we obtained 395 combined sequences of the mitochondrial CO1 and ATP6 markers, which resulted in 17 different haplotypes. We compared a phylogenetic tree constructed from 285 alleles of the nuclear intron ATPsβ to the 17 mitochondrial haplotypes. Congruent patterns between mitochondrial and nuclear gene trees of giant barrel sponges provided evidence for the existence of multiple reproductively isolated species, particularly where they occurred in sympatry. The species complexes in the tropical Atlantic and the Indo-Pacific, however, do not form separate monophyletic lineages. This rules out the scenario that one species of giant barrel sponge developed into separate species complexes following geographic separation and instead suggests that multiple species of giant barrel sponges already existed prior to the physical separation of the Indo-Pacific and tropical Atlantic.

  5. Monitoring core barrel motion by neutron noise diagnostics

    International Nuclear Information System (INIS)

    Por, G.

    1985-08-01

    The core barrel motion is detected by ionization chambers located around the reactor vessel. The method is based on the measurement of the neutron flux fluctuations. Calculations to determine the direction and the size of the motion are discussed. The identification of core barrel motion and its connection with the error of one of the main circulating pumps in the Rheinsberg nuclear power plant are described. Core barrel motion of 10 Hz with an amplitude less than 50 μm could be diagnozed at the Paks-1 reactor using the Dutch high accuracy evaluation system. (V.N.)

  6. Construction and performance of the ATLAS silicon microstrip barrel modules

    International Nuclear Information System (INIS)

    Kondo, T.; Apsimon, R.; Beck, G.A.; Bell, P.; Brenner, R.; Bruckman de Renstrom, P.; Carter, A.A.; Carter, J.R.; Charlton, D.; Dabrowski, W.; Dorholt, O.; Ekelof, T.; Eklund, L.; Gibson, M.; Gadomski, S.; Grillo, A.; Grosse-Knetter, J.; Haber, C.; Hara, K.; Hill, J.C.; Ikegami, Y.; Iwata, Y.; Johansen, L.G.; Kohriki, T.; Macpherson, A.; McMahon, S.; Moorhead, G.; Morin, J.; Morris, J.; Morrissey, M.; Nagai, K.; Nakano, I.; Pater, J.; Pernegger, H.; Perrin, E.; Phillips, P.; Robinson, D.; Skubic, B.; Spencer, N.; Stapnes, S.; Stugu, B.; Takashima, R.; Terada, S.; Tyndel, M.; Ujiie, N.; Unno, Y.; Vos, M.

    2002-01-01

    The ATLAS Semiconductor Tracker (SCT) consists of four barrel cylinders and 18 end-cap disks. This paper describes the SCT modules of the barrel region, of which more than 2000 are about to be constructed. The module design is fixed. Its design concept is given together with the electrical, thermal and mechanical specifications. The pre-series production of the barrel modules is underway using mass-production procedures and jigs. The pre-series modules have given satisfactory performances on noise, noise occupancy, electrical as well as mechanical and thermal properties. In addition, irradiated modules were demonstrated to work successfully. Also first results from a 10-module system test are given

  7. Qualification Procedures of the CMS Pixel Barrel Modules

    CERN Document Server

    Starodumov, A; Horisberger, R.; Kastli, H.Chr.; Kotlinski, D.; Langenegger, U.; Meier, B.; Rohe, T.; Trueb, P.

    2006-01-01

    The CMS pixel barrel system will consist of three layers built of about 800 modules. One module contains 66560 readout channels and the full pixel barrel system about 48 million channels. It is mandatory to test each channel for functionality, noise level, trimming mechanism, and bump bonding quality. Different methods to determine the bump bonding yield with electrical measurements have been developed. Measurements of several operational parameters are also included in the qualification procedure. Among them are pixel noise, gains and pedestals. Test and qualification procedures of the pixel barrel modules are described and some results are presented.

  8. Glycogen synthase kinase-3β inhibition in the medial prefrontal cortex mediates paradoxical amphetamine action in a mouse model of ADHD

    Directory of Open Access Journals (Sweden)

    Yi-Chun eYen

    2015-03-01

    Full Text Available Psychostimulants show therapeutic efficacy in the treatment of attention-deficit hyperactivity disorder (ADHD. It is generally assumed that they ameliorate ADHD symptoms via interfering with monoaminergic signaling. We combined behavioral pharmacology, neurochemistry and molecular analyses to identify mechanisms underlying the paradoxical calming effect of amphetamine in low trait anxiety behavior (LAB mice, a novel multigenetic animal model of ADHD. Amphetamine (1 mg/kg and methylphenidate (10 mg/kg elicited similar dopamine and norepinephrine release in the medial prefrontal cortex (mPFC and in the striatum of LAB mice. In contrast, amphetamine decreased, while methylphenidate increased locomotor activity. This argues against changes in dopamine and/or norepinephrine release as mediators of amphetamine paradoxical effects. Instead, the calming activity of amphetamine corresponded to the inhibition of glycogen synthase kinase3β (GSK3β activity, specifically in the mPFC. Accordingly, not only systemic administration of the GSK3β inhibitor TDZD-8 (20 mg/kg, but also local microinjections of TDZD-8 and amphetamine into the mPFC, but not into the striatum, decreased locomotor activity in LAB mice. Amphetamine effects seem to depend on NMDA receptor signaling, since pre- or co-treatment with MK-801 (0.3 mg/kg abolished the effects of amphetamine (1 mg/kg on the locomotion and on the phosphorylation of GSK3β at the level of the mPFC. Taken together, the paradoxical calming effect of amphetamine in hyperactive LAB mice concurs with a decreased GSK3β activity in the mPFC. This effect appears to be independent of dopamine or norepinephrine release, but contingent on NMDA receptor signaling.

  9. Critical role of types 2 and 3 deiodinases in the negative regulation of gene expression by T₃in the mouse cerebral cortex.

    Science.gov (United States)

    Hernandez, Arturo; Morte, Beatriz; Belinchón, Mónica M; Ceballos, Ainhoa; Bernal, Juan

    2012-06-01

    Thyroid hormones regulate brain development and function through the control of gene expression, mediated by binding of T(3) to nuclear receptors. Brain T(3) concentration is tightly controlled by homeostatic mechanisms regulating transport and metabolism of T(4) and T(3). We have examined the role of the inactivating enzyme type 3 deiodinase (D3) in the regulation of 43 thyroid hormone-dependent genes in the cerebral cortex of 30-d-old mice. D3 inactivation increased slightly the expression of two of 22 positively regulated genes and significantly decreased the expression of seven of 21 negatively regulated genes. Administration of high doses of T(3) led to significant changes in the expression of 12 positive genes and three negative genes in wild-type mice. The response to T(3) treatment was enhanced in D3-deficient mice, both in the number of genes and in the amplitude of the response, demonstrating the role of D3 in modulating T(3) action. Comparison of the effects on gene expression observed in D3 deficiency with those in hypothyroidism, hyperthyroidism, and type 2 deiodinase (D2) deficiency revealed that the negative genes are more sensitive to D2 and D3 deficiencies than the positive genes. This observation indicates that, in normal physiological conditions, D2 and D3 play critical roles in maintaining local T(3) concentrations within a very narrow range. It also suggests that negatively and positively regulated genes do not have the same physiological significance or that their regulation by thyroid hormone obeys different paradigms at the molecular or cellular levels.

  10. Beam tests of the ZEUS barrel calorimeter

    Energy Technology Data Exchange (ETDEWEB)

    Bernstein, A; Bienz, T; Caldwell, A; Chen, L; Derrick, M; Gialas, I; Hamri, A; Imlay, R; Kartik, S; Kim, H J; Kinnel, T; Kreutzmann, H; Li, C G; Lim, J N; Loveless, R; Lu, B; Mallik, U; McLean, K W; McNeil, R; Metcalf, W; Musgrave, B; Oh, B Y; Park, S; Parsons, J A; Reeder, D; Repond, J; Ritz, S; Roco, M T.P.; Sandler, P H; Sciulli, F; Smith, W H; Talaga, R L; Tzanakos, G; Wai, L; Wang, M Z; Whitmore, J; Wu, J; Yang, S [Argonne National Lab., IL (United States) Columbia Univ., New York, NY (United States) Nevis Labs., Irvington-on-Hudson, NY (United States) Univ. of Iowa, Iowa City, IA (United States) Louisiana State Univ., Baton Rouge, LA (United States) Ohio State Univ., Columbus, OH (United States) Pennsylvania State Univ., University Park, PA (United States) Virginia Polytechnic Inst., and State Univ., Blacksburg, VA (United States) Univ. of Wisconsin, Madison, WI (United States)

    1993-11-15

    A fully compensating uranium-scintillator calorimeter was constructed for the ZEUS detector at HERA. Several of the barrel calorimeter modules were subjected to beam tests at Fermilab before shipping them to DESY for installation. The calibrations of the modules used beams of electrons and hadrons, measuring the uniformity of the response, and checking the resolution. The runs also provided opportunity to test a large fraction of the actual ZEUS calorimeter readout system in an integrated beam environment more than one year before HERA turn on. The experiment utilized two computer controlled mechanical structures, one of which was capable of holding up to four modules in order to study shower containment, and a magnetic spectrometer with a high resolution beam tracking system. During two running periods, beams of 6 to 110 GeV containing e, [mu], [pi], and anti p were used. The results show energy resolutions of 35%/[radical]E for hadrons and 19%/[radical]E for electrons, uniformities at the 1% level, energy nonlinearity less than 1%, and equal response for electrons and hadrons. (orig.)

  11. Common barrel and forward CA tracking algorithm

    Energy Technology Data Exchange (ETDEWEB)

    Mykhailo, Pugach [Goethe-Universitaet, Frankfurt (Germany); Frankfurt Institute for Advanced Studies, Frankfurt (Germany); KINR, Kyiv (Ukraine); Gorbunov, Sergey; Kisel, Ivan [Goethe-Universitaet, Frankfurt (Germany); Frankfurt Institute for Advanced Studies, Frankfurt (Germany); Collaboration: PANDA-Collaboration

    2016-07-01

    There are complex detector setups which consist of barrel (cylindrical) and forward parts, and such systems require a special approach in the registered charged particles track finding procedure. Currently the tracking procedure might be performed in both parts of such detector independently from each other, but the final goal on this direction is a creation of a combined tracking, which will work in both parts of the detector simultaneously. The basic algorithm is based on Kalman Filter (KF) and Cellular Automata (CA). And the tracking procedure in such a complex system is rather extraordinary as far as it requires 2 different models to describe the state vector of segments of the reconstructed track in the mathematical apparatus of the KF-algorithm. To overcome this specifics a mathematical apparatus of transition matrices must be developed and implemented, so that one can transfer from one track model to another. Afterwards the work of the CA is performed, which reduces to segments sorting, their union into track-candidates and selection of the best candidates by the chi-square criteria after fitting of the track-candidate by the KF. In this report the algorithm, status and perspectives of such combined tracking are described.

  12. Prototype test for the PANDA barrel DIRC

    Energy Technology Data Exchange (ETDEWEB)

    Dzhygadlo, Roman; Gerhardt, Andreas; Kalicy, Grzegorz; Krebs, Marvin; Lehmann, Dorothe; Schwarz, Carsten; Schwiening, Jochen; Belias, Anastasios; Traxler, Michael [GSI Helmholtzzentrum fur Schwerionenforschung GmbH, Darmstadt (Germany); Peters, Klaus [GSI Helmholtzzentrum fur Schwerionenforschung GmbH, Darmstadt (Germany); Goethe-Universitaet Frankfurt (Germany); Collaboration: PANDA-Collaboration

    2016-07-01

    The Barrel DIRC (Detector of Internally Reflected Cherenkov light) is designed to provide particle identification (PID) for the PANDA experiment at the new Facility for Antiproton and Ion Research in Europe (FAIR) at GSI, Darmstadt. It is based on the successful BABAR DIRC detector with several key improvements, such as focusing optics, fast timing, and a compact expansion volume. A large prototype was constructed and tested in a hadronic particle beam at CERN during the summer of 2015 to test the PID performance of different design options. The prototype included a fused silica radiator (either a narrow bar or a wide plate), an optional focusing lens, and a prism-shaped fused silica expansion volume. An array of microchannel-plate photomultiplier tubes measured the location and arrival time of the Cherenkov photons on 960 pixels. Data were collected for two radiator geometries and several types of focusing lenses at different beam momenta and polar angles. Results of the analysis as well as a comparison to the Geant4 simulation are presented.

  13. Roles of taurine-mediated tonic GABAA receptor activation in the radial migration of neurons in the fetal mouse cerebral cortex

    Directory of Open Access Journals (Sweden)

    Tomonori eFurukawa

    2014-03-01

    Full Text Available γ-Aminobutyric acid (GABA depolarizes embryonic cerebrocortical neurons and continuous activation of the GABAA receptor (GABAAR contributes to their tonic depolarization. Although multiple reports have demonstrated a role of GABAAR activation in neocortical development, including in migration, most of these studies have used pharmacological blockers. Herein, we performed in utero electroporation in GABA synthesis-lacking homozygous GAD67-GFP knock-in mice (GAD67GFP/GFP to label neurons born in the ventricular zone. Three days after electroporation, there were no differences in the distribution of labeled cells between the genotypes. The dose-response properties of cells labeled to detect GABA were equivalent among genotypes. However, continuous blockade of GABAAR with the GABAAR antagonist SR95531 accelerated radial migration. This effect of GABAAR blockade in GAD67GFP/GFP mice suggested a role for alternative endogenous GABAAR agonists. Thus, we tested the role of taurine, which is derived from maternal blood but is abundant in the fetal brain. The taurine-evoked currents in labeled cells were mediated by GABAAR. Taurine uptake was blocked by a taurine transporter inhibitor, 2-(guanidinoethanesulfonic acid (GES, and taurine release was blocked by a volume-sensitive anion channel blocker, 4-(2-butyl-6,7-dichlor-2-cyclopentylindan-1-on-5-yl oxobutyric acid (DCPIB, as examined through high-performance liquid chromatography (HPLC. GES increased the extracellular taurine concentration and induced an inward shift of the holding current, which was reversed by SR95531. In a taurine-deficient mouse model, the GABAAR-mediated tonic currents were greatly reduced, and radial migration was accelerated. As the tonic currents were equivalent among the genotypes of GAD67-GFP knock-in mice, taurine, rather than GABA, might play a major role as an endogenous agonist of embryonic tonic GABAAR conductance, regulating the radial migration of neurons in the

  14. The expression and activity of β-catenin in the thalamus and its projections to the cerebral cortex in the mouse embryo

    Directory of Open Access Journals (Sweden)

    Pratt Thomas

    2012-02-01

    Full Text Available Abstract Background The mammalian thalamus relays sensory information from the periphery to the cerebral cortex for cognitive processing via the thalamocortical tract. The thalamocortical tract forms during embryonic development controlled by mechanisms that are not fully understood. β-catenin is a nuclear and cytosolic protein that transduces signals from secreted signaling molecules to regulate both cell motility via the cytoskeleton and gene expression in the nucleus. In this study we tested whether β-catenin is likely to play a role in thalamocortical connectivity by examining its expression and activity in developing thalamic neurons and their axons. Results At embryonic day (E15.5, the time when thalamocortical axonal projections are forming, we found that the thalamus is a site of particularly high β-catenin mRNA and protein expression. As well as being expressed at high levels in thalamic cell bodies, β-catenin protein is enriched in the axons and growth cones of thalamic axons and its growth cone concentration is sensitive to Netrin-1. Using mice carrying the β-catenin reporter BAT-gal we find high levels of reporter activity in the thalamus. Further, Netrin-1 induces BAT-gal reporter expression and upregulates levels of endogenous transcripts encoding β-actin and L1 proteins in cultured thalamic cells. We found that β-catenin mRNA is enriched in thalamic axons and its 3'UTR is phylogenetically conserved and is able to direct heterologous mRNAs along the thalamic axon, where they can be translated. Conclusion We provide evidence that β-catenin protein is likely to be an important player in thalamocortcial development. It is abundant both in the nucleus and in the growth cones of post-mitotic thalamic cells during the development of thalamocortical connectivity and β-catenin mRNA is targeted to thalamic axons and growth cones where it could potentially be translated. β-catenin is involved in transducing the Netrin-1 signal to

  15. Cocaine and Caffeine Effects on the Conditioned Place Preference Test: Concomitant Changes on Early Genes within the Mouse Prefrontal Cortex and Nucleus Accumbens

    Directory of Open Access Journals (Sweden)

    Javier A. Muñiz

    2017-10-01

    Full Text Available Caffeine is the world's most popular psychostimulant and is frequently used as an active adulterant in many illicit drugs including cocaine. Previous studies have shown that caffeine can potentiate the stimulant effects of cocaine and cocaine-induced drug seeking behavior. However, little is known about the effects of this drug combination on reward-related learning, a key process in the maintenance of addiction and vulnerability to relapse. The goal of the present study was thus to determine caffeine and cocaine combined effects on the Conditioned Place Preference (CPP test and to determine potential differential mRNA expression in the Nucleus Accumbens (NAc and medial prefrontal cortex (mPFC of immediate-early genes (IEGs as well as dopamine and adenosine receptor subunits. Mice were treated with caffeine (5 mg/kg, CAF, cocaine (10 mg/kg, COC, or their combination (caffeine 5 mg/kg + cocaine 10 mg/kg, CAF-COC and trained in the CPP test or treated with repeated injections inside the home cage. NAc and mPFC tissues were dissected immediately after the CPP test, after a single conditioning session or following psychostimulant injection in the home cage for mRNA expression analysis. CAF-COC induced a marked change of preference to the drug conditioned side of the CPP and a significant increase in locomotion compared to COC. Gene expression analysis after CPP test revealed specific up-regulation in the CAF-COC group of Drd1a, cFos, and FosB in the NAc, and cFos, Egr1, and Npas4 in the mPFC. Importantly, none of these changes were observed when animals received same treatments in their home cage. With a single conditioning session, we found similar effects in both CAF and CAF-COC groups: increased Drd1a and decreased cFos in the NAc, and increased expression of Drd1a and Drd2, in the mPFC. Interestingly, we found that cFos and Npas4 gene expression were increased only in the mPFC of the CAF-COC. Our study provides evidence that caffeine acting as

  16. Comparison between two possible CMS Barrel Muon Readout Architectures

    International Nuclear Information System (INIS)

    Aguayo, P.; Barcala, J.M.; Molinero, A.; Pablos, J.L.; Willmott, C.; Alberdi, J.; Marin, J.; Navarrete, J.; Romero, L.

    1997-01-01

    A comparison between two possible readout arquitectures for the CMS muon barrel readout electronics is presented, including various aspects like costs, reliability, installation, staging and maintenance. A review of the present baseline architecture is given in the appendix. (Author)

  17. Tri-Service Green Gun Barrel (PP 1074)

    National Research Council Canada - National Science Library

    Rusch, Lawrence F

    2003-01-01

    ...) PP 1074 Tri-Service Green Gun Barrel. The program's goal was to develop an environmentally friendly process for depositing wear and erosion resistant materials onto gun bores replacing the current hazardous aqueous electro-deposition...

  18. Experimental investigation on shore hardness of barrel-finished ...

    Indian Academy of Sciences (India)

    Rupinder Singh

    2017-08-02

    Aug 2, 2017 ... Barrel finishing (BF) process is widely used to improve the surface finish and dimensional features of metallic and ... plastic-based waste can be easily recycled. However .... The pattern prepared with solid density has a dense.

  19. Integration and installation of the CMS pixel barrel detector

    CERN Document Server

    Kastli, Hans-Christian

    2008-01-01

    A 66 million pixel detector has been installed in 2008 into the CMS experiment at CERN. The development and construction time took more than 10 years. In this paper the assembly of the barrel detector is described. A simple but effective method to accomplish a survey of the module positions during assembly is discussed. Furthermore the insertion and commissioning of the CMS pixel barrel detector which took place in July 2008 is illustrated.

  20. Sodium Dichromate Barrel Landfill expedited response action proposal

    International Nuclear Information System (INIS)

    1993-09-01

    The US Environmental Protection Agency (EPA) and Washington State Department of Ecology (Ecology) recommended that the US Department of Energy (DOE) prepare an expedited response action (ERA) for the Sodium Dichromate Barrel Landfill. The Sodium Dichromate Barrel Disposal Site was used in 1945 for disposal of crushed barrels. The site location is the sole waste site within the 100-IU-4 Operable Unit. The Waste Information Data System (WIDS 1992) assumes that the crushed barrels contained 1% residual sodium dichromate at burial time and that only buried crushed barrels are at the site. Burial depth is shallow since visual inspection finds numerous barrel debris on the surface. A non-time-critical ERA proposal includes preparation of an engineering evaluation and cost analysis (EE/CA) section. The EE/CA is a rapid, focused evaluation of available technologies using specific screening factors to assess feasibility, appropriateness, and cost. The ERA goal is to reduce the potential for any contaminant migration from the landfill to the soil column, groundwater, and Columbia River. Since the landfill is the only waste site within the operable unit, the ERA will present a final remediation of the 100-IU-4 operable unit

  1. First two barrel ECAL supermodules inserted in CMS HCAL

    CERN Multimedia

    K.Bell

    2006-01-01

    The first two barrel "supermodules" for the CMS Electromagnetic Calorimeter (ECAL) have been inserted into the barrel hadron calorimeter (HCAL) in the experimental hall (called SX5) in Cessy in preparation for the forthcoming magnet test and cosmic challenge (MTCC). Each of the two supermodules contains 1700 lead tungstate crystals in glass-fibre alveolar support structures, with associated avalanche photodiodes (APDs, for scintillation light detection), electronics and cooling system. The barrel ECAL will consist of 36 supermodules, many of which have already been produced (see CERN Bulletin 17-18, 2006). Team from CMS ECAL, CMS Integration and CEA-DAPNIA were involved in the insertion, with the production/integration of the supermodules themselves involving many technicians, engineers and physicists from many institutes. From left to right: Olivier Teller, Maf Alidra and Lucien Veillet.

  2. Correcting the Chromatic Aberration in Barrel Distortion of Endoscopic Images

    Directory of Open Access Journals (Sweden)

    Y. M. Harry Ng

    2003-04-01

    Full Text Available Modern endoscopes offer physicians a wide-angle field of view (FOV for minimally invasive therapies. However, the high level of barrel distortion may prevent accurate perception of image. Fortunately, this kind of distortion may be corrected by digital image processing. In this paper we investigate the chromatic aberrations in the barrel distortion of endoscopic images. In the past, chromatic aberration in endoscopes is corrected by achromatic lenses or active lens control. In contrast, we take a computational approach by modifying the concept of image warping and the existing barrel distortion correction algorithm to tackle the chromatic aberration problem. In addition, an error function for the determination of the level of centroid coincidence is proposed. Simulation and experimental results confirm the effectiveness of our method.

  3. Mechanical Commissioning of the ATLAS Barrel Toroid Magnet

    CERN Document Server

    Foussat, A; Dudarev, A; Bajas, H; Védrine, P; Berriaud, C; Sun, Z; Sorbi, M

    2008-01-01

    ATLAS is a general-purpose detector designed to run at the highest luminosity at the CERN Large Hadron Collider. Its features include the 4 T Barrel Toroid magnet, the largest superconducting magnet (25 m long, 20 m diameter) that provides the magnetic field for the ATLAS muon spectrometer. The coils integrated at CERN, were tested individually at maximum current of 22 kA in 2005. Following the mechanical assembly of the Barrel Toroid in the ATLAS underground cavern, the test of the full Barrel Toroid was performed in October 2006. Further tests are foreseen at the end 2007 when the system will include the two End Cap Toroids (ECT). The paper gives an overview of the good mechanical test results achieved in comparison with model predictions and the experience gained in the mechanical behavior of the ATLAS Toroidal coils is discussed.

  4. Thalamocortical control of feed-forward inhibition in awake somatosensory 'barrel' cortex.

    OpenAIRE

    Swadlow, Harvey A

    2002-01-01

    Intracortical inhibition plays a role in shaping sensory cortical receptive fields and is mediated by both feed-forward and feedback mechanisms. Feed-forward inhibition is the faster of the two processes, being generated by inhibitory interneurons driven by monosynaptic thalamocortical (TC) input. In principle, feed-forward inhibition can prevent targeted cortical neurons from ever reaching threshold when TC input is weak. To do so, however, inhibitory interneurons must respond to TC input at...

  5. Effect analysis of core barrel openings under CEFR normal condition

    International Nuclear Information System (INIS)

    Zhang Yabo; Yang Hongyi

    2008-01-01

    Openings on the bottom of core barrel are important part of the decay heat removal system of China Experimental Fast Reactor (CEFR), which are designed to discharge the decay heat from reactor under accident condition. This paper analyses the effect of the openings design on the normal operation condition using the famouse CFD code CFX. The result indicates that the decay heat can be discharged safely and at the same time the effect of core barrel openings on the normal operation condition is acceptable. (authors)

  6. LHC-rate beam test of CMS pixel barrel modules

    International Nuclear Information System (INIS)

    Erdmann, W.; Hoermann, Ch.; Kotlinski, D.; Horisberger, R.; Kaestli, H. Chr.; Gabathuler, K.; Bertl, W.; Meier, B.; Langenegger, U.; Trueeb, P.; Rohe, T.

    2007-01-01

    Modules for the CMS pixel barrel detector have been operated in a high rate pion beam at PSI in order to verify under LHC-like conditions the final module design for the production. The test beam provided charged particle rates up to 10 8 cm -2 s -1 over the full module area. Bunch structure and randomized high trigger rates simulated realistic operation. A four layer telescope made of single pixel readout chip assemblies provided tracking needed for the determination of the modules hit reconstruction efficiency. The performance of the modules has been shown to be adequate for the CMS pixel barrel

  7. End of the EM Barrel Presampler Construction and Insertion

    CERN Multimedia

    Hostachy, J.Y.

    The liquid argon barrel presampler is a thin detector placed in front of the electromagnetic barrel calorimeter, made up of two half barrels also, but with 32 sectors per half barrel instead of 16. Each of these 64 sectors is 3.1 m long, 28 cm large and 2.9 cm thick. Three countries took part in its construction: France (LPSC-Grenoble), Sweden (KTH-Stockholm) and Morocco (Hassan II Ain Chock-Casablanca and Mohamed V-Rabat universities, and CNESTEN-Rabat). The design of the presampler started 11 years ago and the series production began at the end of 2000. Cabling, mechanical and electronic tests of the anodes were achieved in Morocco. Forty-one sectors were assembled and validated at the LPSC-Grenoble and 25 at the KTH-Stockholm. In November 2002, the first half was inserted on the inner face of the first EM calorimeter wheel. The insertion of the other 32 sectors in the second EM calorimeter wheel was achieved in July 2003 (see pictures). The production of two additional sectors will allow us to study the p...

  8. barrel temperature emperature emperature effects on the ffects

    African Journals Online (AJOL)

    eobe

    Keywords: barrel temperature,plastic products, injection moulding machine , tensile strength, flexual strength. 1. INTRODUCTION. INTRODUCTION. INTRODUCTION. The utilization of process control and process monitoring are rarely fully implemented for the production of injection moulded products. This may be due to a ...

  9. Results of 3D photogrammetry on the CMS barrel yoke

    International Nuclear Information System (INIS)

    Goudard, R.; Humbertclaude, C.; Nummiaro, K.

    1999-01-01

    The CMS (Compact Muon Solenoid) detector of the new LHC will be built till 2005 at CERN in Geneva. The Barrel Yoke survey has been decided to be done mostly by photogrammetry. After this first measurement, it has been proved that a practical simulation and a study of adapted tools and procedures were helpful for measurement on such a large object. Using only conventional surveying methods would have been impossible with such constraints. The most important points were the high required accuracy compared to the size of the object, the connection of the two planes, the time intervention, the restricted factory environment and the impossibility for having any outside network. The photogrammetric method was considered to be the best way to survey the Barrel Yoke ring. Since the required precision has been reached at all levels with the first full Barrel measurements, the procedure is validated for the four remaining Barrels in the factory and afterwards again at CERN. This project was challenging due to the size of the object, the required accuracy and the lack of practical references in the field of digital industrial photogrammetry. This method is a new step for using the three dimensional photogrammetric measurements on large objects. (authors)

  10. ATLAS barrel hadron tile calorimeter: spacers plates mass production

    International Nuclear Information System (INIS)

    Artikov, A.M.; Budagov, Yu.A.; Khubua, J.

    1999-01-01

    In this article we expose the main problems of the mass production of the so-called 'spacer plates' for the ATLAS Barrel Hadron Tile Calorimeter. We describe all practical solutions of these problems. Particularly we present the measurement procedures and calculation schemes we used for the spacers dimensions determination. The results of the calculations are presented

  11. Experiences developing socially acceptable interactions for a robotic trash barrel

    DEFF Research Database (Denmark)

    Yang, Stephen; Mok, Brian Ka Jun; Sirkin, David

    2015-01-01

    strategies that seemed to evoke clear engagement and responses, both positive and negative. Observations and interviews show that a) people most welcome the robot's presence when they need its services and it actively advertises its intent through movement; b) people create mental models of the trash barrel...

  12. The barrel modules of the ATLAS semiconductor tracker

    Czech Academy of Sciences Publication Activity Database

    Abdesselam, A.; Akimoto, T.; Allport, P.; Böhm, Jan; Šťastný, Jan

    2006-01-01

    Roč. 568, - (2006), s. 642-671 ISSN 0168-9002 Institutional research plan: CEZ:AV0Z10100502 Keywords : ATLAS * SCT * silicon * microstrip * module * LHC * barrel Subject RIV: BF - Elementary Particles and High Energy Physics Impact factor: 1.185, year: 2006

  13. New results in meson spectroscopy from the crystal barrel experiment

    Energy Technology Data Exchange (ETDEWEB)

    Meyer, C.A. [Carnegie Mellon Univ., Pittsburgh, PA (United States)

    1994-04-01

    Recent observations by the Crystal Barrel experiment of two scalar resonances, f{sub o}(1365) and a{sub o}(1450) have allowed the authors to clarify the members of the scalar nonet. In addition, a third scalar, f{sub o}(1500), appears to be supernumerary, and is a candidate for the scalar glueball expected near 1500 MeV.

  14. Monitoring of the ageing of the DELPHI Barrel RICH MWPCs

    International Nuclear Information System (INIS)

    Apeldoorn, G.; Buys, A.; Dracos, M.; Toet, D.Z.

    1996-01-01

    We describe a method to monitor ageing effects in the multiwire proportional chambers (MWPCs) of the barrel ring imaging Cherenkov (BRICH) detector of DELPHI. It is based on a measurement of plateau-curve characteristics and the shift of these as a function of the total collected charge. Results indicate that, as yet, no ageing effects are present. (orig.)

  15. Disruption of the LTD dialogue between the cerebellum and the cortex in Angelman syndrome model: a timing hypothesis

    Directory of Open Access Journals (Sweden)

    Guy eCheron

    2014-11-01

    Full Text Available Angelman syndrome is a genetic neurodevelopmental disorder in which cerebellar functioning impairment has been documented despite the absence of gross structural abnormalities. Characteristically, a spontaneous 160 Hz oscillation emerges in the Purkinje cells network of the Ube3am-/p+ Angelman mouse model. This abnormal oscillation is induced by enhanced Purkinje cell rhythmicity and hypersynchrony along the parallel fiber beam. We present a pathophysiological hypothesis for the neurophysiology underlying major aspects of the clinical phenotype of Angelman syndrome, including cognitive, language and motor deficits, involving long-range connection between the cerebellar and the cortical networks. This hypothesis states that the alteration of the cerebellar rhythmic activity impinges cerebellar long-term depression (LTD plasticity, which in turn alters the LTD plasticity in the cerebral cortex. This hypothesis was based on preliminary experiments using electrical stimulation of the whiskers pad performed in alert mice showing that after a 8 Hz LTD-inducing protocol, the cerebellar LTD accompanied by a delayed response in the wild type mice is missing in Ube3am-/p+ mice and that the LTD induced in the barrel cortex following the same peripheral stimulation in wild mice is reversed into a LTP in the Ube3am-/p+ mice. The control exerted by the cerebellum on the excitation vs inhibition balance in the cerebral cortex and possible role played by the timing plasticity of the Purkinje cell LTD on the spike–timing dependent plasticity (STDP of the pyramidal neurons are discussed in the context of the present hypothesis.

  16. The integration and engineering of the ATLAS SemiConductor Tracker Barrel

    Energy Technology Data Exchange (ETDEWEB)

    Abdesselam, A; Barr, A J [Department of Physics, Oxford University, Denys Wilkinson Building, Keble Road, Oxford OX1 3RH (United Kingdom); Allport, P P; Austin, N [Oliver Lodge Laboratory, University of Liverpool, P.O. Box 147, Oxford Street, Liverpool L69 3BX (United Kingdom); Anastopoulos, C [University of Sheffield, Department of Physics and Astronomy, Hounsfield Road, Sheffield S3 7RH (United Kingdom); Anderson, B; Attree, D J [Department of Physics and Astronomy, University College London (United Kingdom); Andricek, L; Bangert, A [Max-Planck-Institut fuer Physik, (Werner-Heisenberg-Institut), Foehringer Ring 6, 80805 Muenchen (Germany); Anghinolfi, F [CERN, CH - 1211 Geneva 23 (Switzerland); Apsimon, R; Barclay, P; Batchelor, L E [Rutherford Appleton Laboratory, Science and Technology Facilities Council, Harwell Science and Innovation Campus, Didcot OX11 0QX (United Kingdom); Atkinson, T [School of Physics, University of Melbourne, Parkville, Victoria 3010 (Australia); Barbier, G [Universite de Geneve, Section de Physique, 24 rue Ernest Ansermet, CH - 1211 Geneve 4 (Switzerland); Bates, R L; Bell, W H [University of Glasgow, Department of Physics and Astronomy, Glasgow G12 8QQ (United Kingdom); Batley, J R [Cavendish Laboratory, University of Cambridge, J J Thomson Avenue, Cambridge CB3 0HE (United Kingdom); Beck, G A [Department of Physics, Queen Mary, University of London, Mile End Road, London E1 4NS (United Kingdom); Bell, P J [School of Physics and Astronomy, University of Manchester, Manchester M13 9PL (United Kingdom)] (and others)

    2008-10-15

    The ATLAS SemiConductor Tracker (SCT) was built in three sections: a barrel and two end-caps. This paper describes the design, construction and final integration of the barrel section. The barrel is constructed around four nested cylinders that provide a stable and accurate support structure for the 2112 silicon modules and their associated services. The emphasis of this paper is directed at the aspects of engineering design that turned a concept into a fully-functioning detector, as well as the integration and testing of large sub-sections of the final SCT barrel detector. The paper follows the chronology of the construction. The main steps of the assembly are described with the results of intermediate tests. The barrel service components were developed and fabricated in parallel so that a flow of detector modules, cooling loops, opto-harnesses and Frequency-Scanning-Interferometry (FSI) alignment structures could be assembled onto the four cylinders. Once finished, each cylinder was conveyed to the next site for the mounting of modules to form a complete single barrel. Extensive electrical and thermal function tests were carried out on the completed single barrels. In the next stage, the four single barrels and thermal enclosures were combined into the complete SCT barrel detector so that it could be integrated with the Transition Radiation Tracker (TRT) barrel to form the central part of the ATLAS inner detector. Finally, the completed SCT barrel was tested together with the TRT barrel in noise tests and using cosmic rays.

  17. Effect of Panax notoginseng saponins on the expression of beta-amyloid protein in the cortex of the parietal lobe and hippocampus, and spatial learning and memory in a mouse model of senile dementia

    Institute of Scientific and Technical Information of China (English)

    Zhenguo Zhong; Dengpan Wu; Liang Lü; Jinsheng Wang; Wenyan Zhang; Zeqiang Qu

    2008-01-01

    BACKGROUND: The pharmacological actions of Panax notoginseng saponins (PNS) lie in removing free radicals, anti-inflammation and anti-oxygenation. It can also improve memory and behavior in rat models of Alzheimer's disease.OBJECTIVE: Using the Morris water maze, immunohistochemistry, real-time PCR and RT-PCR, this study aimed to measure improvement in spatial learning, memory, expression of amyloid precursor protein (App) and β -amyloid (A β ), to investigate the mechanism of action of PNS in the treatment of AD in the senescence accelerated mouse-prone 8 (SAMP8) and compare the effects with huperzine A.DESIGN, TIME AND SETTING: A completely randomized grouping design, controlled animal experiment was performed in the Center for Research & Development of New Drugs, Guangxi Traditional Chinese Medical University from July 2005 to April 2007.MATERIALS: Sixty male SAMP8 mice, aged 3 months, purchased from Tianjin Chinese Traditional Medical University of China, were divided into four groups: PNS high-dosage group, PNS low-dosage group,huperzine A group and control group. PNS was provided by Weihe Pharmaceutical Co., Ltd. (batch No.:Z53021485, Yuxi, Yunan Province, China). Huperzine A was provided by Zhenyuan Pharmaceutical Co., Ltd.(batch No.: 20040801, Zhejiang. China).METHODS: The high-dosage group and low-dosage group were treated with 93.50 and 23.38 mg/kg PNS respectively per day and the huperzine A group was treated with 0.038 6 mg/kg huperzine A per day, all by intragastric administration, for 8 consecutive weeks. The same volume of double distilled water was given to the control group.MAIN OUTCOME MEASURES: After drug administration, learning and memory abilities were assessed by place navigation and spatial probe tests. The recording indices consisted of escape latency (time-to-platform), and the percentage of swimming time spent in each quadrant. The number of A β1-40,A β1-42 and App immunopositive neurons in the brains of SAMP8 mice was analyzed by

  18. ATLAS barrel toroid integration and test area in building 180

    CERN Multimedia

    Maximilien Brice

    2003-01-01

    The ATLAS barrel toroid system consists of eight coils, each of axial length 25.3 m, assembled radially and symmetrically around the beam axis. The coils are of a flat racetrack type with two 'double-pancake' windings made of 20.5 kA aluminium-stabilized niobium-titanium superconductor. The barrel toroid is being assembled in building 180 on the Meyrin site. In the first phase of assembly, the coils are packed into their aluminium-alloy casing. These photos show the double-pancake coils from ANSALDO and the coil casings from ALSTOM. In the foreground is the tooling from COSMI used to turn over the coil casings during this first phase. In the right background is the yellow lifting gantry manufactured at JINR-Dubna, Russia which will transport the coil casings to a heating table for prestressing. Two test benches with magnetic mirror are also visible.

  19. The bar PANDA Barrel-TOF Detector at FAIR

    Science.gov (United States)

    Zimmermann, S.; Suzuki, K.; Steinschaden, D.; Chirita, M.; Ahmed, G.; Dutta, K.; Kalita, K.; Lehmann, A.; Böhm, M.; Schwarz, K.; Orth, H.; Brinkmann, K.-Th.

    2017-08-01

    The barrel-Time-of-Flight subdetector is one of the outer layers of the multi-layer design of the \\panda target spectrometer. It is designed with a minimal material budget in mind mainly consisting of 90×30×5 mm3 thin plastic scintillator tiles read out on each end by a serial connection of 4 SiPMs. 120 such tiles are placed on 16 2460 × 180 mm2 PCB boards forming a barrel covering an azimuthal angle from 22.5o to 150o. The detector is designed to achieve a time resolution below σ< 100 ps which allows to distinguish events in the constant stream of hits, as well as particle identification below the Cherenkov threshold via the time-of-flight; simultaneously providing the interaction times of events. The current prototype achieved a time resolution of ~54 ps, well below the design goal.

  20. Simulgeo and its application for the muon barrel position monitor

    International Nuclear Information System (INIS)

    Brunel, L.

    1999-01-01

    The design process of the Muon Barrel Position Monitor of the CMS (compact muon solenoid) experiment for LHC is at the origin of the need of a software like Simulgeo. The software Silmugeo started to be developed in 1995 in order to allow the study of many systems. The idea of Simulgeo is to automatically make the modelling of a system and automatically construct the design matrix. This paper makes in part 2 an overview of the possibilities of Simulgeo, in part 3 it presents the standard objects. In part 4, it explains the mathematical basis and in part 5 the computing aspect. In part 6, it shows an application to the Muon Barrel Position Monitor Project and, in part 7, it mentions other projects where it has been used

  1. Design of the LHC US ATLAS Barrel Cryostat

    CERN Document Server

    Rehak, M L; Farah, Y; Grandinetti, R; Müller, T; Norton, S; Sondericker, J

    2002-01-01

    One of the experiments of CERN's Large Hadron Collider (LHC) is the ATLAS Liquid Argon detector. The Liquid Argon Barrel Cryostat is part of the United States contribution to the LHC project and its design is presented here. The device is made up of four concentric cylinders: the smallest and largest of which form a vacuum vessel enclosing a cold vessel cryostat filled with liquid argon. The Cryostat serves as the housing for an electromagnetic barrel calorimeter, supports and provides space in vacuum for a solenoid magnet while the toroidal opening furnishes room for a tracker detector. Design requirements are determined by its use in a collider experiment: the construction has to be compact, the material between the interaction region and the calorimeter has to be minimal and made of aluminum to reduce the amount of absorbing material. The design complies with code regulations while being optimized for its use in a physics environment. (2 refs).

  2. Signal feedthroughs for the ATLAS barrel and endcap calorimeters

    International Nuclear Information System (INIS)

    Axen, D.; Hackenburg, R.; Hoffmann, A.; Kane, S.; Lissauer, D.; Makowiecki, D.; Muller, T.; Pate, D.; Radeka, V.; Rahm, D.; Rehak, M.; Rescia, S.; Sexton, K.; Sondericker, J.; Birney, P.; Dowling, A.W.; Fincke-Keeler, M.; Hodges, T.; Holness, F.; Honkanen, N.

    2005-01-01

    The function, design, construction, testing, and installation of the signal feedthroughs for the barrel and endcap ATLAS liquid argon calorimeters are described. The feedthroughs provide a high density and radiation hard method to extract over 200 000 signals from the cryogenic environment of the calorimeters using an application of a design based on flexible kapton circuit board transmission lines. A model to describe the frequency dependent behavior of the transmission lines is also presented

  3. Reversible man-in-the-barrel syndrome in myasthenia gravis

    OpenAIRE

    Shah, Poornima A; Wadia, Pettarusp Murzban

    2016-01-01

    Man-in-the-barrel syndrome (MBS) is an uncommon presentation due to bilateral, predominantly proximal muscle weakness that has not been described to be associated with myasthenia gravis. We describe a case of myasthenia gravis presenting as MBS. Additionally, he had significant wasting of the deltoids bilaterally with fibrillations on electromyography (EMG) at rest and brief duration (3-6 ms) bi/triphasic motor unit potentials (MUPs) on submaximal effort apart from a decremental response on r...

  4. Device for cementing radioactive of toxic waste into barrels

    International Nuclear Information System (INIS)

    Hempelmann, W.; Waldenmeier, G.; Mathis, P.; Mathis, B.; Mathis, F.

    1987-01-01

    The plant consists of conventional means, such as through mixers and dosing and transport spirals, which are accommodated in a glovebox. The inactive additives cement and sand and the active materials evaporation concentrates and sludges are mixed by them, and are then filled into a waste barrel which is empty or already filled with solid waste. Liquid radioactive wastes are used to concrete over the solid waste. (DG) [de

  5. Design and construction of the ZEUS barrel calorimeter

    International Nuclear Information System (INIS)

    Repond, J.

    1990-01-01

    The mechanical design and construction techniques of the barrel calorimeter for the ZEUS detector are presented. The calorimeter uses alternate layers of depleted uranium and scintillator with one radiation length sampling. The unit cell has e/h = 1 which yields an optimal energy resolution for hadronic jets. We discuss the placing of the structural components and cracks between modules. Details of the construction and assembly effort needed to realize the total calorimeter are reported. 4 figs., 1 tab

  6. Mechanical structure of the TOPAZ barrel drift chamber

    International Nuclear Information System (INIS)

    Morimoto, T.; Maruyama, K.; Okuno, H.

    1987-07-01

    A Barrel Drift Chamber (BDC) is constructed for the TOPAZ experiment at TRISTAN, KEK. The BDC has a cylindrical shape with dimensions of 325.2 cm in inner diameter, 347.2 cm in outer diameter and 500 cm long. It consists of 1232 drift tubes made of conductive plastic cathodes, which are staggered in four layers. In this report, a design of the mechanical structure and construction procedures are described in detail. (author)

  7. Non-compensation of the ATLAS barrel combined calorimeter prototype

    International Nuclear Information System (INIS)

    Kul'chitskij, Yu.A.; Kuz'min, M.V.

    1998-01-01

    The e / π ratio for the ATLAS Barrel Combined Calorimeter Prototype, composed from electromagnetic LArg calorimeter and hadronic Tile calorimeter was investigated. Response of Combined Calorimeter on pions and electrons in the energy region of 20-300 GeV was studied. Found e / h = 1.37 ± 0.01 ± 0.02 is in good agreement with the results from previous Combined Calorimeter tests but has more precisions

  8. Optical properties of the PANDA barrel DIRC radiator bars

    Energy Technology Data Exchange (ETDEWEB)

    Kalicy, Grzegorz; Krebs, Marvin; Peters, Klaus [GSI, Darmstadt (Germany); Goethe Universitaet, Frankfurt (Germany); Schwarz, Carsten; Schwiening, Jochen [GSI, Darmstadt (Germany); Collaboration: PANDA-Collaboration

    2015-07-01

    The PANDA experiment at the Facility for Antiproton and Ion Research in Europe (FAIR) at GSI, Darmstadt, will study fundamental questions of hadron physics and QCD. A fast focusing DIRC (Detection of Internally Reflected Cherenkov light) counter will provide hadronic particle identification (PID) in the barrel region of the PANDA detector. To meet the PID requirements, the Barrel DIRC has to provide precise measurements of the Cherenkov angle, which is conserved for Cherenkov photons propagating through the radiator by total internal reflection. The radiators, rectangular bars made from fused silica, have to fulfill very strict optical and mechanical requirements. This includes the squareness and parallelism of the sides of the bars, sharp corners, and a very smooth surface polish, ensuring that the Cherenkov photons reach the optical sensors without angular distortions. Currently the Barrel DIRC is at the final design stage and several different bar shapes and fabrication methods are being considered for the final detector. An optical setup, consisting of a computer-controlled positioning and a multi-wavelength laser system, is used to evaluate the radiator bars to obtain critical values like transmittance and reflectivity. The current results and techniques are presented on this poster.

  9. Spiking in primary somatosensory cortex during natural whisking in awake head-restrained rats is cell-type specific

    NARCIS (Netherlands)

    de Kock, C.P.J.; Sakmann, B.

    2009-01-01

    Sensation involves active movement of sensory organs, but it remains unknown how position or movement of sensory organs is encoded in cortex. In the rat whisker system, each whisker is represented by an individual cortical (barrel) column. Here, we quantified in awake, head-fixed rats the impact of

  10. Energy metrics for driving competitiveness of countries: Energy weakness magnitude, GDP per barrel and barrels per capita

    International Nuclear Information System (INIS)

    Coccia, Mario

    2010-01-01

    Energy metrics is the development of a whole new theoretical framework for the conception and measurement of energy and economic system performances, energy efficiency and productivity improvements with important political economy implications consistent with the best use of all natural and economic resources. The purpose of this research is to present some vital energy indicators based on magnitude and scale of energy weakness, GDP per barrel of oil that is an indicator of energy productivity and barrels (of oil) per capita that is an indicator of energy efficiency. Energy metrics can support the monitoring of energy and economic system performances in order to design effective energy strategy and political economy interventions focused on the 'competitive advantage' increase of countries in modern economies.

  11. The barrel muon spectrometer of the ATLAS detector has acquired its first cosmic event in a magnetic field produced by the barrel toroid magnet.

    CERN Multimedia

    2006-01-01

    A 3-D event display of a cosmic muon event, showing the path of a muon travelling through three layers of the barrel muon spectrometer. Three of the eight coils of the barrel toroid magnet can be seen in the top half of the drawing.

  12. Reversible man-in-the-barrel syndrome in myasthenia gravis

    Directory of Open Access Journals (Sweden)

    Poornima A Shah

    2016-01-01

    Full Text Available Man-in-the-barrel syndrome (MBS is an uncommon presentation due to bilateral, predominantly proximal muscle weakness that has not been described to be associated with myasthenia gravis. We describe a case of myasthenia gravis presenting as MBS. Additionally, he had significant wasting of the deltoids bilaterally with fibrillations on electromyography (EMG at rest and brief duration (3-6 ms bi/triphasic motor unit potentials (MUPs on submaximal effort apart from a decremental response on repetitive nerve stimulation (RNS at 2 Hz. While electrophysiology is an important tool in the diagnosis of myasthenia gravis, pathological EMG patterns do not exclude the diagnosis of myasthenia gravis.

  13. Reversible man-in-the-barrel syndrome in myasthenia gravis

    Science.gov (United States)

    Shah, Poornima A; Wadia, Pettarusp Murzban

    2016-01-01

    Man-in-the-barrel syndrome (MBS) is an uncommon presentation due to bilateral, predominantly proximal muscle weakness that has not been described to be associated with myasthenia gravis. We describe a case of myasthenia gravis presenting as MBS. Additionally, he had significant wasting of the deltoids bilaterally with fibrillations on electromyography (EMG) at rest and brief duration (3-6 ms) bi/triphasic motor unit potentials (MUPs) on submaximal effort apart from a decremental response on repetitive nerve stimulation (RNS) at 2 Hz. While electrophysiology is an important tool in the diagnosis of myasthenia gravis, pathological EMG patterns do not exclude the diagnosis of myasthenia gravis. PMID:27011638

  14. Postnatal Development of CB1 Receptor Expression in Rodent Somatosensory Cortex

    Science.gov (United States)

    Deshmukh, Suvarna; Onozuka, Kaori; Bender, Kevin J.; Bender, Vanessa A.; Lutz, Beat; Mackie, Ken; Feldman, Daniel E.

    2007-01-01

    Endocannabinoids are powerful modulators of synaptic transmission that act on presynaptic cannabinoid receptors. Cannabinoid receptor type 1 (CB1) is the dominant receptor in the CNS, and is present in many brain regions, including sensory cortex. To investigate the potential role of CB1 receptors in cortical development, we examined the developmental expression of CB1 in rodent primary somatosensory (barrel) cortex, using immunohistochemistry with a CB1-specific antibody. We found that before postnatal day (P) 6, CB1 receptor staining was present exclusively in the cortical white matter, and that CB1 staining appeared in the grey matter between P6 and P20 in a specific laminar pattern. CB1 staining was confined to axons, and was most prominent in cortical layers 2/3, 5a, and 6. CB1 null (−/−) mice showed altered anatomical barrel maps in layer 4, with enlarged inter-barrel septa, but normal barrel size. These results indicate that CB1 receptors are present in early postnatal development and influence development of sensory maps. PMID:17210229

  15. Fenomen erozije cevi naoružanja / Gun barrel erosion

    Directory of Open Access Journals (Sweden)

    Slobodan Ilić

    2009-07-01

    Full Text Available U radu su prikazana dosadašnja istraživanja erozije cevi. Trošenje cevi je neizbežan proces do kojeg dolazi pri svakom opaljenju. Erozija cevi može se definisati kao progresivna povreda ili oštećenje površine cevi i proširenje kalibra usled opaljenja, koja utiče na: gubitak ili pad početne brzine projektila, tačnost i efektivnost oruđa. Kao glavni uzročnici koji utiču na intenzitet erozije cevi opisani su termički, hemijski i mehanički faktori. Njihov uticaj prikazan je u interakciji cev-projektil-barutno punjenje. / An overview of research into gun barrel erosion is presented in this paper. The gun bore wear is an unavoidable process during normal firing. The wear can be defined as progressive damage of the bore surface and enlargement of the bore, ultimately resulting in loss in the muzzle velocity, range and accuracy. Thermal, chemical and mechanical factors are described as major factors of gun barrel erosion. The influence of these factors is presented in the interaction of gun tube - projectile - propellant.

  16. Recent results from the DELPHI barrel ring imaging Cherenkov counter

    International Nuclear Information System (INIS)

    Anassontzis, E.G.; Ioannou, P.; Kalkanis, G.; Katsanevas, S.; Kontaxis, I.; Kourkoumelis, C.; Nounos, S.; Preve, P.; Resvanis, L.K.; Brunet, J.M.; Dolbeau, J.; Guglielmo, L.; Ledroit, F.; Poutot, D.; Tristram, G.

    1991-01-01

    The DELPHI detector, installed at LEP, is equipped with RICH (Ring Imaging Cherenkov) counters. The Barrel part incorporates a liquid (C 6 F 14 ) and a gaseous (C 5 F 12 ) radiator providing particle identification up to 20GeV/c. The Cherenkov protons of both radiators are detected by TPC-like photon detectors. The drift gas (75% CH 4 + 25% C 2 H 6 ) is doped with TMAE, but which the UV Cherenkov photons are converted into single free photo-electrons. These are drifted towards MWPC's at the end of the drift tubes and the space coordinates of the conversion point are determined. One half of the Barrel RICH is now equipped with drift tubes and has provided results from the liquid radiator since spring 1990. The gas radiator has been tested with C 2 F 6 as a preliminary filling since August 1990. The data obtained demonstrate the good particle identification potential. For the liquid radiator the number of detected photons per ring in hadron jets is N=8, whereas for muon pairs (single tracks) N=10 has been obtained. For the gas radiator 2.1 photons per track were observed, which demonstrates the good functioning of the focussing mirrors, as the C 2 F 6 this is close to the expected value

  17. First physics pulses in the Barrel Electromagnetic Calorimeter with cosmics

    CERN Multimedia

    Laurent Serin

    2006-01-01

    The electromagnetic barrel calorimeter has been installed in its final position in October 2005. Since then, the calorimeter is being equipped with front-end electronics. Starting in April 2006, electronics calibration runs are taken a few times per week to debug the electronics and to study the performance in the pit (stability, noise). Today, 10 out of the 32 Front End crates are being read out, amounting to about 35000 channels. cool down, few little typos --> After a 6-week cool down, the barrel cryostat was filled with Liquid Argon in May. The presence of a few shorts (~1MΩ) at the edges of the modules was indicating the possibility of conducting dust having entered into the calorimeter with the flowing liquid. In order to try to improve this situation, the calorimeter was emptied and filled again, but this time by condensating the argon instead of flowing it in liquid phase. The new High Voltage tests are not showing any significant improvement but the situation is statisfactory for ATLAS runn...

  18. Installation of CMS EB (ECAL Barrel) Supermodules 5 and 13 inside HB+ (HCAL Barrel) on 26/27 April 2006

    CERN Multimedia

    Ken Bell, RAL

    2006-01-01

    The first two barrel "supermodules" of the CMS Electromagnetic Calorimeter (ECAL) have been inserted into the barrel hadron calorimeter (HCAL) in the CMS experimental hall (called SX5) in Cessy in preparation for the forthcoming magnet test and cosmic challenge (MTCC). Each of the two supermodules contains 1700 lead tungstate crystals in glass-fibre alveolar support structures, with associated avalanche photodiodes (APDs, for scintillation light detection), electronics and cooling system. See also the document CMS-PHO-OREACH-2006-019. The first two pictures show the two supermodules in their final position. Fig. 3: the "enfourneur" in position on the HB Cradle. Fig. 4: supermodule n. 5 and extension rails being lifted to the enforneur. Figs. 5-6: supermodule approaching the enforneur. Fig. 7: rotating the Enfourneur to the correct phi direction Figs. 8-9: aligning the extension rails with the rails inside HB and view from inside HB, once the rails are aligned. Figs. 10-12: insertion of supermodule n. 5. Fig. ...

  19. Design and development of a work robot to place ATLAS SCT modules onto barrel cylinders

    International Nuclear Information System (INIS)

    Terada, S.; Kobayashi, H.; Sengoku, H.; Kato, Y.; Hara, K.; Honma, F.; Ikegami, Y.; Iwata, Y.; Kohriki, T.; Kondo, T.; Nakano, I.; Takashima, R.; Tanaka, R.; Ujiie, N.; Unno, Y.; Yasuda, S.

    2005-01-01

    More than 2000 silicon modules need to be placed and fastened on the ATLAS SCT barrel tracker. A semi-automatic pick-and-place work robot was designed and developed to cope with the module placement for the SCT barrel assembly. We found that this robot could place modules to a mechanical precision of better than 25 μm

  20. Fabrication and Tests of M240 Machine Gun Barrels Lined with Stellite 25

    Science.gov (United States)

    2016-04-01

    ARL-TR-7662 ● APR 2016 US Army Research Laboratory Fabrication and Tests of M240 Machine Gun Barrels Lined with Stellite 25...Fabrication and Tests of M240 Machine Gun Barrels Lined with Stellite 25 by William S de Rosset and Sean Fudger Weapons and Materials Research...

  1. Design and development of a work robot to place ATLAS SCT modules onto barrel cylinders

    CERN Document Server

    Terada, S; Honma, F; Ikegami, Y; Iwata, Y; Kato, Y; Kobayashi, H; Kohriki, T; Kondo, T; Nakano, I; Sengoku, H; Takashima, R; Tanaka, R; Ujiie, N; Unno, Y; Yasuda, S

    2005-01-01

    More than 2000 silicon modules need to be placed and fastened on the ATLAS SCT barrel tracker. A semi-automatic pick-and-place work robot was designed and developed to cope with the module placement for the SCT barrel assembly. We found that this robot could place modules to a mechanical precision of better than 25 mum.

  2. Shell model in-water frequencies of the core barrel

    International Nuclear Information System (INIS)

    Takeuchi, K.; De Santo, D.F.

    1980-01-01

    Natural frequencies of a 1/24th-scale core barrel/vessel model in air and in water are measured by determining frequency responses to applied forces. The measured data are analyzed by the use of the one-dimensional fluid-structure computer code, MULTIFLEX, developed to calculate the hydraulic force. The fluid-structure interaction in the downcomer annulus is computed with a one-dimensional network model formed to be equivalent to two-dimensional fluid-structure interaction. The structural model incorporated in MULTIFLEX is substantially simpler than that necessary for structural analyses. Proposed for computation of structural dynamics is the projector method than can deal with the beam mode by modal analysis and the other shell modes by a direct integration method. Computed in-air and in-water frequencies agree fairly well with the experimental data, verifying the above MULTIFLEX technique

  3. The CMS Level-1 Trigger Barrel Track Finder

    International Nuclear Information System (INIS)

    Ero, J.; Wulz, C.; Evangelou, I.; Flouris, G.; Foudas, C.; Loukas, N.; Manthos, N.; Papadopoulos, I.; Paradas, E.; Guiducci, L.; Sotiropoulos, S.; Sphicas, P.; Triossi, A.

    2016-01-01

    The design and performance of the upgraded CMS Level-1 Trigger Barrel Muon Track Finder (BMTF) is presented. Monte Carlo simulation data as well as cosmic ray data from a CMS muon detector slice test have been used to study in detail the performance of the new track finder. The design architecture is based on twelve MP7 cards each of which uses a Xilinx Virtex-7 FPGA and can receive and transmit data at 10 Gbps from 72 input and 72 output fibers. According to the CMS Trigger Upgrade TDR the BMTF receives trigger primitive data which are computed using both RPC and DT data and transmits data from a number of muon candidates to the upgraded Global Muon Trigger. Results from detailed studies of comparisons between the BMTF algorithm results and the results of a C++ emulator are also presented. The new BMTF will be commissioned for data taking in 2016

  4. Manufacturing aspects of the ATLAS barrel toroid double pancakes

    CERN Document Server

    Drago, G; Gagliardi, P; Laurenti, A; Marabotto, R; Penco, R

    2002-01-01

    In 1999 INFN (Istituto Nazionale di Fisica Nucleare) ordered to ANSALDO the manufacturing of 16 double pancakes for the ATLAS BARREL TOROID. In July 2001 four Double Pancakes have already been completed and shipped to the integration site. In this paper the main aspects of the manufacturing of the largest superconducting coils ever built (5*25 m) are described. The main phases of the manufacturing procedure are reviewed starting from the conductor preparation to the VPI impregnation, including references to the materials used as well as to the relevant customer's requirements. In particular the special winding form and the winding technique are treated. For each phase the most critical aspects and the relevant solutions are pointed out. Particular details about the technical solutions adopted for the impregnation and curing of the Double Pancake, which could not be performed inside an autoclave due to the huge dimension of the coil itself, are reported. Finally the methods used for the dimensional and electri...

  5. Effect of Friction on Barreling during cold Upset Forging of Aluminium 6082 Alloy Solid cylinders

    Science.gov (United States)

    Priyadarshini, Amrita; Kiran, C. P.; Suresh, K.

    2018-03-01

    Friction is one of the significant factors in forging operations since it affects metal flow in the die, forming load, strain distribution, tool and die life, surface quality of the product etc. In upset forging, the frictional forces at the die-workpiece interface oppose the outward flow of the material due to which the specimen develops a barrel shape. As a result, the deformation becomes non-uniform or inhomogeneous which is undesirable. Barreling can be reduced by applying effective lubricant on the surface of the platens. The objective of the present work is to study experimentally the effect of various frictional conditions (dry, grease, mineral oil) on barreling during upset forging of aluminum 6082 solid cylinders of different aspect ratio (length/diameter: 0.5, 0.75, 1). The friction coefficients are determined using the ring compression test. Curvature of barrel is determined based on the assumption that the curvature of the barrel follows the geometry of circular arc.

  6. Study and analysis for the flow-induced vibration of the core barrel of a PWR

    International Nuclear Information System (INIS)

    Yao Weida; Shi Guolin; Jiang Nanyan

    1989-01-01

    The resemblance criteria are derived and a test model is designed by applying the flow-soild coupling theory. After having completed the model analysis of the pressurized water reactor (PWR) core barrel in an 1:10 model, the dynamic characteristics are obtained. In an 1:5 reactor model with a hydraulic closed loop, the hydraulic vibration tests of the core barrel are performed, and the relations between the flow rate and the flow-induced pulse pressure on core barrel, acceleration and strain signals have been measured. The corresponding responses and a group of computational equations for hydraulic vibration are derived from these two experiments. The computational hydraulic vibration responses for core barrel in Qinshan Nuclear Power Plant are in good agreement with the test results, and it shows that the core barrel is safe within its lifetime of 30 years

  7. Assembly of 5.5-Meter Diameter Developmental Barrel Segments for the Ares I Upper Stage

    Science.gov (United States)

    Carter, Robert W.

    2011-01-01

    Full scale assembly welding of Ares I Upper Stage 5.5-Meter diameter cryogenic tank barrel segments has been performed at the Marshall Space Flight Center (MSFC). One full-scale developmental article produced under the Ares 1 Upper Stage project is the Manufacturing Demonstration Article (MDA) Barrel. This presentation will focus on the welded assembly of this barrel section, and associated lessons learned. Among the MDA articles planned on the Ares 1 Program, the Barrel was the first to be completed, primarily because the process of manufacture from piece parts (barrel panels) utilized the most mature friction stir process planned for use on the Ares US program: Conventional fixed pin Friction Stir Welding (FSW). This process is in use on other space launch systems, including the Shuttle s External Tank, the Delta IV common booster core, the Delta II, and the Atlas V rockets. The goals for the MDA Barrel development were several fold: 1) to prove out Marshall Space Flight Center s new Vertical Weld Tool for use in manufacture of cylindrical barrel sections, 2) to serve as a first run for weld qualification to a new weld specification, and 3) to provide a full size cylindrical section for downstream use in precision cleaning and Spray-on Foam Insulation development. The progression leading into the welding of the full size barrel included sub scale panel welding, subscale cylinder welding, a full length confidence weld, and finally, the 3 seamed MDA barrel processing. Lessons learned on this MDA program have been carried forward into the production tooling for the Ares 1 US Program, and in the use of the MSFC VWT in processing other large scale hardware, including two 8.4 meter diameter Shuttle External Tank barrel sections that are currently being used in structural analysis to validate shell buckling models.

  8. Unimodal primary sensory cortices are directly connected by long-range horizontal projections in the rat sensory cortex

    Directory of Open Access Journals (Sweden)

    Jimmy eStehberg

    2014-09-01

    Full Text Available Research based on functional imaging and neuronal recordings in the barrel cortex subdivision of primary somatosensory cortex (SI of the adult rat has revealed novel aspects of structure-function relationships in this cortex. Specifically, it has demonstrated that single whisker stimulation evokes subthreshold neuronal activity that spreads symmetrically within gray matter from the appropriate barrel area, crosses cytoarchitectural borders of SI and reaches deeply into other unimodal primary cortices such as primary auditory (AI and primary visual (VI. It was further demonstrated that this spread is supported by a spatially matching underlying diffuse network of border-crossing, long-range projections that could also reach deeply into AI and VI. Here we seek to determine whether such a network of border-crossing, long-range projections is unique to barrel cortex or characterizes also other primary, unimodal sensory cortices and therefore could directly connect them. Using anterograde (BDA and retrograde (CTb tract-tracing techniques, we demonstrate that such diffuse horizontal networks directly and mutually connect VI, AI and SI. These findings suggest that diffuse, border-crossing axonal projections connecting directly primary cortices are an important organizational motif common to all major primary sensory cortices in the rat. Potential implications of these findings for topics including cortical structure-function relationships, multisensory integration, functional imaging and cortical parcellation are discussed.

  9. Circadian oscillators in the mouse brain

    DEFF Research Database (Denmark)

    Rath, Martin F; Rovsing, Louise; Møller, Morten

    2014-01-01

    with conditional cell-specific clock gene deletions. This prompted us to analyze the molecular clockwork of the mouse neocortex and cerebellum in detail. Here, by use of in situ hybridization and quantitative RT-PCR, we show that clock genes are expressed in all six layers of the neocortex and the Purkinje...... and granular cell layers of the cerebellar cortex of the mouse brain. Among these, Per1, Per2, Cry1, Arntl, and Nr1d1 exhibit circadian rhythms suggesting that local running circadian oscillators reside within neurons of the mouse neocortex and cerebellar cortex. The temporal expression profiles of clock genes...... are similar in the neocortex and cerebellum, but they are delayed by 5 h as compared to the SCN, suggestively reflecting a master-slave relationship between the SCN and extra-hypothalamic oscillators. Furthermore, ARNTL protein products are detectable in neurons of the mouse neocortex and cerebellum...

  10. The TIM Barrel Architecture Facilitated the Early Evolution of Protein-Mediated Metabolism.

    Science.gov (United States)

    Goldman, Aaron David; Beatty, Joshua T; Landweber, Laura F

    2016-01-01

    The triosephosphate isomerase (TIM) barrel protein fold is a structurally repetitive architecture that is present in approximately 10% of all enzymes. It is generally assumed that this ubiquity in modern proteomes reflects an essential historical role in early protein-mediated metabolism. Here, we provide quantitative and comparative analyses to support several hypotheses about the early importance of the TIM barrel architecture. An information theoretical analysis of protein structures supports the hypothesis that the TIM barrel architecture could arise more easily by duplication and recombination compared to other mixed α/β structures. We show that TIM barrel enzymes corresponding to the most taxonomically broad superfamilies also have the broadest range of functions, often aided by metal and nucleotide-derived cofactors that are thought to reflect an earlier stage of metabolic evolution. By comparison to other putatively ancient protein architectures, we find that the functional diversity of TIM barrel proteins cannot be explained simply by their antiquity. Instead, the breadth of TIM barrel functions can be explained, in part, by the incorporation of a broad range of cofactors, a trend that does not appear to be shared by proteins in general. These results support the hypothesis that the simple and functionally general TIM barrel architecture may have arisen early in the evolution of protein biosynthesis and provided an ideal scaffold to facilitate the metabolic transition from ribozymes, peptides, and geochemical catalysts to modern protein enzymes.

  11. Syria: a waited petroleum production of 600 000 barrels per day for the end of 1993

    International Nuclear Information System (INIS)

    Anon.

    1993-01-01

    This paper describes the reserves of petroleum deposits and gives statistical data on the production of the main oil fields in Syria. Petroleum production has grown from 160000 barrels per day in 1984 to 515000 barrels per day in 1992 and a production at a level of 600000 barrels per day is waited in 1993. Several petroleum companies, operating in Syria, have contributed to this rise, especially Al-Furat Petroleum Company (AFPC), joint venture between Syrian Petroleum Company (SPC) and Pecten/Shell/Deminex, followed by Deiz Ezzor Petroleum Company (DEZPC), association between the french company Elf Aquitaine and SPC. 2 tabs

  12. Vinten exposure measurements of the Salem Unit 1 lower core barrel

    International Nuclear Information System (INIS)

    Glennon, P.T.

    1988-01-01

    On November 6, 1987, the lower core barrel of Salem Unit I was removed from the reactor vessel and placed in the refueling pool as part of the unit's ten year inspection program. This paper deals with the supporting actions of the dosimetry group of PSE ampersand G. Prior to the move of the lower core barrel, Westinghouse predicted dose rates at one foot in water as a function of axial distance along the core barrel. This prediction was used in planning the health physics requirements associated with the move. It was agreed that a measurement of the axial dose rates would either lend confidence to the predictions or identify weaknesses in them

  13. Second Barrel Toroid Coil Installed in ATLAS Cavern

    CERN Multimedia

    Tappern, G.

    The second barrel toroid coil was lowered into the ATLAS Cavern on Friday, 26 November. The operation takes approximately five hours of precision crane and winch operations. Before lowering, several checks are made to ensure that no loose items have been left on the coil which would fall during the lowering down the shaft. This is a very difficult, but very important check, with the first coil in position, and partly below the shaft. After changing the winch tooling on Wednesday December 1st, the coil was lifted, rotated and placed into the feet. The girders which support the coil and the Z direction stops had all been pre-set before putting the coil in the feet. The angle is controlled by an inclinometer. When the final adjustments of position have been made, which will locate the coils at the plus/minus two mm level, the connection beams (voussoirs and struts) will be put in place; this requires a complex shimming procedure. This will lock together the two coils into the feet and forms the foundation for th...

  14. First ATLAS Barrel Toroid coil casing arrives at CERN

    CERN Multimedia

    2002-01-01

    The first of eight 25-metre long coil casings for the ATLAS experiment's barrel toroid magnet system arrived at CERN on Saturday 2 March by road from Heidelberg. This structure will be part of the largest superconducting toroid magnet ever made.   The first coil casing for the toroidal magnets of Atlas arrives at Building 180. This is the start of an enormous three-dimensional jigsaw puzzle. Each of the eight sets of double pancake coils will be housed inside aluminium coil casings, which in turn will be held inside a stainless steel vacuum vessel. A huge construction, the casing that arrived at CERN measures 25 metres in length and 5 metres in width. It weighs 20 tones. And this is just the beginning of the toroid jigsaw: by early April a batch of four double pancake coils, which altogether weighs 65 tones, will arrive from Ansaldo in Italy. The first vacuum vessel will also be arriving from Felguera in Spain this month. It will take about two years for all these 25 m long structures of casings, coils a...

  15. Double Barreled Wet Colostomy: Initial Experience and Literature Review

    Science.gov (United States)

    Salgado-Cruz, Luis; Espin-Basany, Eloy; Vallribera-Valls, Francesc; Sanchez-Garcia, Jose; Jimenez-Gomez, Luis Miguel; Marti-Gallostra, Marc; Garza-Maldonado, Ana

    2014-01-01

    Background. Pelvic exenteration and multivisceral resection in colorectal have been described as a curative and palliative intervention. Urinary tract reconstruction in a pelvic exenteration is achieved in most cases with an ileal conduit of Bricker, although different urinary reservoirs have been described. Methods. A retrospective and observational study of six patients who underwent a pelvic exenteration and urinary tract reconstruction with a double barreled wet colostomy (DBWC) was done, describing the preoperative diagnosis, the indication for the pelvic exenteration, the complications associated with the procedure, and the followup in a period of 5 years. A literature review of the case series reported of the technique was performed. Results. Six patients had a urinary tract reconstruction with the DBWC technique, 5 male patients and one female patient. Age range was from 20 to 77 years, with a medium age 53.6 years. The most frequent complication presented was a pelvic abscess in 3 patients (42.85%); all complications could be resolved with a conservative treatment. Conclusion. In the group of our patients with pelvic exenteration and urinary tract reconstruction with a DBWC, it is a safe procedure and well tolerated by the patients, and most of the complications can be resolved with conservative treatment. PMID:25574498

  16. Energy Resolution of the Barrel of the CMS Electromagnetic Calorimeter

    CERN Document Server

    Adzic, Petar; Almeida, Carlos; Almeida, Nuno; Anagnostou, Georgios; Anfreville, Marc; Anicin, Ivan; Antunovic, Zeljko; Auffray, Etiennette; Baccaro, Stefania; Baffioni, Stephanie; Baillon, Paul; Barney, David; Barone, Luciano; Barrillon, Pierre; Bartoloni, Alessandro; Beauceron, Stephanie; Beaudette, Florian; Bell, Ken W; Benetta, Robert; Bercher, Michel; Berthon, Ursula; Betev, Botjo; Beuselinck, Raymond; Bhardwaj, Ashutosh; Bialas, Wojciech; Biino, Cristina; Bimbot, Stephane; Blaha, Jan; Bloch, Philippe; Blyth, Simon; Bordalo, Paula; Bornheim, Adolf; Bourotte, Jean; Britton, David; Brown, Robert M; Brunelière, Renaud; Busson, Philippe; Camporesi, Tiziano; Cartiglia, Nicolo; Cavallari, Francesca; Cerutti, Muriel; Chamont, David; Chang, Paoti; Chang, You-Hao; Charlot, Claude; Chatterji, Sudeep; Chen, E Augustine; Chipaux, Rémi; Choudhary, Brajesh C; Cockerill, David J A; Collard, Caroline; Combaret, Christophe; Cossutti, Fabio; Da Silva, J C; Dafinei, Ioan; Daskalakis, Georgios; Davatz, Giovanna; Decotigny, David; De Min, Alberto; Deiters, Konrad; Dejardin, Marc; Del Re, Daniele; Della Negra, Rodolphe; Della Ricca, Giuseppe; Depasse, Pierre; Descamp, J; Dewhirst, Guy; Dhawan, Satish; Diemoz, Marcella; Dissertori, Günther; Dittmar, Michael; Djambazov, Lubomir; Dobrzynski, Ludwik; Drndarevic, Snezana; Dupanloup, Michel; Dzelalija, Mile; Ehlers, Jan; El-Mamouni, H; Peisert, Anna; Evangelou, Ioannis; Fabbro, Bernard; Faure, Jean-Louis; Fay, Jean; Ferri, Federico; Flower, Paul S; Franzoni, Giovanni; Funk, Wolfgang; Gaillac, Anne-Marie; Gargiulo, Corrado; Gascon-Shotkin, S; Geerebaert, Yannick; Gentit, François-Xavier; Ghezzi, Alessio; Gilly, Jean; Giolo-Nicollerat, Anne-Sylvie; Givernaud, Alain; Gninenko, Sergei; Go, Apollo; Godinovic, Nikola; Golubev, Nikolai; Golutvin, Igor; Gómez-Reino, Robert; Govoni, Pietro; Grahl, James; Gras, Philippe; Greenhalgh, Justin; Guillaud, Jean-Paul; Haguenauer, Maurice; Hamel De Montechenault, G; Hansen, Magnus; Heath, Helen F; Hill, Jack; Hobson, Peter R; Holmes, Daniel; Holzner, André; Hou, George Wei-Shu; Ille, Bernard; Ingram, Quentin; Jain, Adarsh; Jarry, Patrick; Jauffret, C; Jha, Manoj; Karar, M A; Kataria, Sushil Kumar; Katchanov, V A; Kennedy, Bruce W; Kloukinas, Kostas; Kokkas, Panagiotis; Korjik, M; Krasnikov, Nikolai; Krpic, Dragomir; Kyriakis, Aristotelis; Lebeau, Michel; Lecomte, Pierre; Lecoq, Paul; Lemaire, Marie-Claude; Lethuillier, Morgan; Lin, Willis; Lintern, A L; Lister, Alison; Litvin, V; Locci, Elizabeth; Lodge, Anthony B; Longo, Egidio; Loukas, Demetrios; Luckey, D; Lustermann, Werner; Lynch, Clare; MacKay, Catherine Kirsty; Malberti, Martina; Maletic, Dimitrije; Mandjavidze, Irakli; Manthos, Nikolaos; Markou, Athanasios; Mathez, Hervé; Mathieu, Antoine; Matveev, Viktor; Maurelli, Georges; Menichetti, Ezio; Meridiani, Paolo; Milenovic, Predrag; Milleret, Gérard; Miné, Philippe; Mur, Michel; Musienko, Yuri; Nardulli, Alessandro; Nash, Jordan; Neal, Homer; Nédélec, Patrick; Negri, Pietro; Nessi-Tedaldi, Francesca; Newman, Harvey B; Nikitenko, Alexander; Obertino, Maria Margherita; Ofierzynski, Radoslaw Adrian; Organtini, Giovanni; Paganini, Pascal; Paganoni, Marco; Papadopoulos, Ioannis; Paramatti, Riccardo; Pastrone, Nadia; Pauss, Felicitas; Puljak, Ivica; Pullia, Antonino; Puzovic, Jovan; Ragazzi, Stefano; Ramos, Sergio; Rahatlou, Shahram; Rander, John; Ranjan, Kirti; Ravat, Olivier; Raymond, M; Razis, Panos A; Redaelli, Nicola; Renker, Dieter; Reucroft, Steve; Reymond, Jean-Marc; Reynaud, Michel; Reynaud, Serge; Romanteau, Thierry; Rondeaux, Françoise; Rosowsky, André; Rovelli, Chiara; Rumerio, Paolo; Rusack, Roger; Rusakov, Sergey V; Ryan, Matthew John; Rykaczewski, Hans; Sakhelashvili, Tariel; Salerno, Roberto; Santos, Marcelino; Seez, Christopher; Semeniouk, Igor; Sharif, Omar; Sharp, Peter; Shepherd-Themistocleous, Claire; Shevchenko, Sergey; Shivpuri, Ram Krishen; Sidiropoulos, Georgios; Sillou, Daniel; Singovsky, Alexander; Sirois, Y; Sirunyan, Albert M; Smith, Brian; Smith, Vincent J; Sproston, Martin; Suter, Henry; Swain, John; Tabarelli de Fatis, Tommaso; Takahashi, Maiko; Tapper, Robert J; Tcheremoukhine, Alexandre; Teixeira, Isabel; Teixeira, Joao Paulo; Teller, Olivier; Timlin, Claire; Triantis, F A; Troshin, Sergey; Tyurin, Nikolay; Ueno, Koji; Uzunian, Andrey; Varela, Joao; Vaz-Cardoso, N; Verrecchia, Patrice; Vichoudis, Paschalis; Vigano, S; Viertel, Gert; Virdee, Tejinder; Vlassov, E; Wang, Minzu; Weinstein, Alan; Williams, Jennifer C; Yaselli, Ignacio; Zabi, Alexandre; Zamiatin, Nikolai; Zelepoukine, Serguei; Zeller, Michael E; Zhang, Lin; Zhang, Jia-Wen; Zhang, Yawei; Zhu, Kejun; Zhu, Ren-Yuan

    2007-01-01

    The energy resolution of the barrel part of the CMS Electromagnetic Calorimeter has been studied using electrons of 20 to 250 GeV in a test beam. The incident electron's energy was reconstructed by summing the energy measured in arrays of 3x3 or 5x5 channels. There was no significant amount of correlated noise observed within these arrays. For electrons incident at the centre of the studied 3x3 arrays of crystals, the mean stochastic term was measured to be 2.8% and the mean constant term to be 0.3%. The amount of the incident electron's energy which is contained within the array depends on its position of incidence. The variation of the containment with position is corrected for using the distribution of the measured energy within the array. For uniform illumination of a crystal with 120 GeV electrons a resolution of 0.5% was achieved. The energy resolution meets the design goal for the detector.

  17. Cryogenic Characteristics of the ATLAS Barrel Toroid Superconducting Magnet

    CERN Document Server

    Pengo, R; Delruelle, N; Pezzetti, M; Pirotte, O; Passardi, Giorgio; Dudarev, A; ten Kate, H

    2008-01-01

    ATLAS, one of the experiments of the LHC accelerator under commissioning at CERN, is equipped with a large superconducting magnet the Barrel Toroid (BT) that has been tested at nominal current (20500 A). The BT is composed of eight race-track superconducting coils (each one weights about 45 tons) forming the biggest air core toroidal magnet ever built. By means of a large throughput centrifugal pump, a forced flow (about 10 liter/second at 4.5 K) provides the indirect cooling of the coils in parallel. The paper describes the results of the measurements carried out on the complete cryogenic system assembled in the ATLAS cavern situated 100 m below the ground level. The measurements include, among other ones, the static heat loads, i.e., with no or constant current in the magnet, and the dynamic ones, since additional heat losses are produced, during the current ramp-up or slow dump, by eddy currents induced on the coil casing.

  18. Some developments in core-barrel vibration diagnostics

    International Nuclear Information System (INIS)

    Pazsit, I.; Karlsson, J.; Garis, N.S.

    1998-01-01

    Diagnostics of core-barrel motion, and notably that of beam mode vibrations, has been usually performed by two distinct concepts. One strategy is to perform a qualitative analysis in the time domain, using descriptors such as vibration trajectory, probability distributions etc. This approach is rather realistic in the sense that it allows for general anisotropic pendular vibrations. The other strategy is to use frequency analysis with the goal of quantifying certain vibration properties. However, this second approach could so far handle only isotropic and unidirectional vibrations. In this paper we propose a unification of these two approaches by introducing a model by which general anisotropic vibrations can be quantified in the frequency domain. However, when separating the noise components prior to the frequency analysis, we suggest the use of symmetry properties of the noise in the time domain, based on reactor physics assumptions, as opposed to the earlier methods that use statistical independence of the components. Due to the unified approach, a combination of time and frequency domain analysis methods can be used for presentation and maximum information extraction

  19. Barrel Toroid fully charged to nominal field, and it works!

    CERN Multimedia

    Herman ten Kate

    After a few weeks of testing up to intermediate currents, finally, on Thursday evening November 9, the current in the Barrel Toroid was pushed up to its nominal value of 20500 A and even 500 A beyond this value to prove that we have some margin. It went surprisingly well. Of course, the 8 coils forming the toroid were already tested individually at the surface but still, some surprise may have come from those parts added to the toroid in the cavern for the first time like the 8 cryoring sections linking the coils as well as the valve box at the bottom in sector 13 regulating the helium flow or the current lead cryostat on the top in sector 5. No training quenches, nothing to worry about, and the test was concluded with a fast dump triggered at 00:40 in the very early morning of November 10. (left) The toroid current during the evening and night of November 9. (right) The test crew oscillated between fear and hope while looking at the control panels as the current approached 21kA. Big relief was in the...

  20. A Front-End Readout Architecture for the CMS Barrel Muon Detector: A Feasibility Study

    International Nuclear Information System (INIS)

    Aguayo, P.; Alberdi, J.; Barcala, J.M.; Marin, J.; Molinero, A.; Navarrete, J.; Pablos, J.L. de; Romero, L.; Willmot, C.

    1995-01-01

    A feasibility study of a possible architecture for the CMS barrel muon detector readout electronics is presented. some aspects of system reliability are discussed. Values for the required FIFO's to store data during the first level trigger latency are given

  1. Study and analysis on the flow induced vibration of the core barrel of PWR

    International Nuclear Information System (INIS)

    Yao Weida; Shi Guolin; Jiang Nanyan; Peng YongYong; Zhang Huijun; Wang Yufen; Xie Yongcheng; Guo Chunhua; Shen Qinping

    1989-01-01

    The deduction of the resemblance criterion and the design of the test model by applying flow-solid coupling theory are described. The model analysis of a core barrel both in the air and stationary water were performed in a 1:10 model, thus obtaining the dynamic characteristic. In a 1:5 reactor model with a hydraulic closed loop, the inner structure and support were modeled for performing hydraulic closed loop, the inner structure and support were modeled for performing hydraulic vibration test of the core barrel. The flow induced pulse pressure of the core barrel and corresponding response were obtained by using miniature pressure capsule, strain gauge and accelerometer. Power spectrum, correlation functions, transfer function and amplitudes under different flow velocities were calculated. The hydraulic vibration test shows that the core barrel will be in safety during its 30-year life time

  2. Modeling two-phase flow in barrels of weapons with combined charges

    Directory of Open Access Journals (Sweden)

    Nebojša P. Hristov

    2011-10-01

    Full Text Available The processes occurring during the firing within barrels of weapons with combined charges are described aiming at the improvement of fire power of existing weapons and the design of new ones. The firing process simulation enables the optimization of gunpowders. The analysis of the obtained results helps in choosing the best combination of input-output parameters for the highest muzzle velocity possible while keeping powder gas maximum pressures inside the barrel at the lowest possible level.

  3. The composite barrel of retail prices and its relationship to crude oil prices

    International Nuclear Information System (INIS)

    Balabanoff, S.

    1993-01-01

    This paper challenges assumptions about the relationship between refinery gate prices, retail prices paid by consumers and crude oil prices. The analysis presented here considers their relationship within the context of the Organization of Petroleum Exporting Countries' (OPEC's) composite barrel statistics, which includes taxes and other government policy effects on prices. Speed of adjustment and retail price response to taxes are analysed with respect to crude import prices. OPEC's composite barrel is explained and evaluated. Test results are summarized. (UK)

  4. Criticality safety of storage barrels for enriched uranium fresh fuel at the RB research reactor

    International Nuclear Information System (INIS)

    Pesic, M. P.

    1997-01-01

    Study on criticality safety of fresh low and high enriched uranium (LEU and HEU) fuel elements in the storage/transport barrels at the RB research reactor is carried out by using the well-known MCNP computer code. It is shown that studied arrays of tightly closed fuel barrels, each entirely loaded with 100 fresh (HEU or LEU) fuel slugs, are far away from criticality, even in cases of an unexpected flooding by light water.(author)

  5. Monitoring of core barrel vibrations in WWER type reactor using out-of-reactor ionization chambers

    International Nuclear Information System (INIS)

    Dach, K.

    1982-01-01

    Vibration of the core barrel is least desirable for safe operation of the PWR reactor. These mechanical vibrations are in correlation with the fluctuations of neutron flux density whose time and frequency analysis serves failure diagnosis. The mathematical model is described of the transfer of mechanical vibrations of the core barrel to neutron noise. Other steps are indicated indispensable for the application of the method of neutron noise analysis for in-service diagnostics of nuclear power plants. (Z.M.)

  6. Energy calibration of the barrel calorimeter of the CMD-3 detector

    International Nuclear Information System (INIS)

    Anisenkov, A.V.; Aulchenko, V.M.; Bashtovoy, N.S.; Bondar, A.E.; Grebenuk, A.A.; Epifanov, D.A.; Epshteyn, L.B.; Erofeev, A.L.; Kovalenko, O.A.; Kozyrev, A.N.; Kuzmin, A.S.; Mikhailov, K.Yu.; Logashenko, I.B.; Razuvaev, G.P.; Ruban, A.A.; Shebalin, V.E.; Shwartz, B.A.; Talyshev, A.A.; Titov, V.M.; Yudin, Yu.V.

    2017-01-01

    The VEPP-2000 e + e − collider has been operated in the Budker Institute of Nuclear Physics since 2010. Experiments are carried out with two detectors CMD-3 and SND. The calorimetry at the CMD-3 detector is based on three subsystems, two coaxial barrel calorimeters—Liquid Xenon calorimeter and crystal CsI calorimeter, and end cap calorimeter with BGO crystals. This paper describes the procedures of the energy calibration of the combined barrel calorimeter of the CMD-3 detector.

  7. First half-barrel of the CMS hadron calorimeter successfully asembled

    CERN Multimedia

    2001-01-01

    The first half barrel of the CMS hadron calorimeter has been assembled in the CMS construction hall in Cessy (neighbouring France), called SX5, in October 2001. The picture sequence shows the insertion of the last (the keystone) wedge. It is lifted up to the top of the structure and carefully inserted into the half barrel. Photos 6 and 7 show the HB- in SX5.

  8. Fluid and structural dynamics calculations to determine core barrel loads during blowdown (EV 3,000)

    International Nuclear Information System (INIS)

    Krieg, R.; Schlechtendahl, E.G.

    1977-01-01

    To begin with, the main physical phenomena in connection with blowdown loads on the care barrel and the computer models used are briefly described. These models have also been used in the design of the HTR test care barrel. The fluid dynamics part of the calculations was carried out using the WHAMMOD and DAPSY codes; for the structural dynamics part, the STRUDL/Dynal code was employed. (orig./RW) [de

  9. Evolutions of volatile sulfur compounds of Cabernet Sauvignon wines during aging in different oak barrels.

    Science.gov (United States)

    Ye, Dong-Qing; Zheng, Xiao-Tian; Xu, Xiao-Qing; Wang, Yun-He; Duan, Chang-Qing; Liu, Yan-Lin

    2016-07-01

    The evolution of volatile sulfur compounds (VSCs) in Cabernet Sauvignon wines from seven regions of China during maturation in oak barrels was investigated. The barrels were made of different wood grains (fine and medium) and toasting levels (light and medium). Twelve VSCs were quantified by GC/FPD, with dimethyl sulfide (DMS) and methionol exceeding their sensory thresholds. Most VSCs tended to decline during the aging, while DMS was found to increase. After one year aging, the levels of DMS, 2-methyltetrahy-drothiophen-3-one and sulfur-containing esters were lower in the wines aged in oak barrels than in stainless steel tanks. The wood grain and toasting level of oak barrels significantly influenced the concentration of S-methyl thioacetate and 2-methyltetrahy-drothiophen-3-one. This study reported the evolution of VSCs in wines during oak barrel aging for the first time and evaluated the influence of barrel types, which would provide wine-makers with references in making proposals about wine aging. Copyright © 2016 Elsevier Ltd. All rights reserved.

  10. Neural cell adhesion molecule, NCAM, regulates thalamocortical axon pathfinding and the organization of the cortical somatosensory representation in mouse

    Science.gov (United States)

    Enriquez-Barreto, Lilian; Palazzetti, Cecilia; Brennaman, Leann H.; Maness, Patricia F.; Fairén, Alfonso

    2012-01-01

    To study the potential role of neural cell adhesion molecule (NCAM) in the development of thalamocortical (TC) axon topography, wild type, and NCAM null mutant mice were analyzed for NCAM expression, projection, and targeting of TC afferents within the somatosensory area of the neocortex. Here we report that NCAM and its α-2,8-linked polysialic acid (PSA) are expressed in developing TC axons during projection to the neocortex. Pathfinding of TC axons in wild type and null mutant mice was mapped using anterograde DiI labeling. At embryonic day E16.5, null mutant mice displayed misguided TC axons in the dorsal telencephalon, but not in the ventral telencephalon, an intermediate target that initially sorts TC axons toward correct neocortical areas. During the early postnatal period, rostrolateral TC axons within the internal capsule along the ventral telencephalon adopted distorted trajectories in the ventral telencephalon and failed to reach the neocortex in NCAM null mutant animals. NCAM null mutants showed abnormal segregation of layer IV barrels in a restricted portion of the somatosensory cortex. As shown by Nissl and cytochrome oxidase staining, barrels of the anterolateral barrel subfield (ALBSF) and the most distal barrels of the posteromedial barrel subfield (PMBSF) did not segregate properly in null mutant mice. These results indicate a novel role for NCAM in axonal pathfinding and topographic sorting of TC axons, which may be important for the function of specific territories of sensory representation in the somatosensory cortex. PMID:22723769

  11. Sensory cortex underpinnings of traumatic brain injury deficits.

    Directory of Open Access Journals (Sweden)

    Dasuni S Alwis

    Full Text Available Traumatic brain injury (TBI can result in persistent sensorimotor and cognitive deficits including long-term altered sensory processing. The few animal models of sensory cortical processing effects of TBI have been limited to examination of effects immediately after TBI and only in some layers of cortex. We have now used the rat whisker tactile system and the cortex processing whisker-derived input to provide a highly detailed description of TBI-induced long-term changes in neuronal responses across the entire columnar network in primary sensory cortex. Brain injury (n=19 was induced using an impact acceleration method and sham controls received surgery only (n=15. Animals were tested in a range of sensorimotor behaviour tasks prior to and up to 6 weeks post-injury when there were still significant sensorimotor behaviour deficits. At 8-10 weeks post-trauma, in terminal experiments, extracellular recordings were obtained from barrel cortex neurons in response to whisker motion, including motion that mimicked whisker motion observed in awake animals undertaking different tasks. In cortex, there were lamina-specific neuronal response alterations that appeared to reflect local circuit changes. Hyper-excitation was found only in supragranular layers involved in intra-areal processing and long-range integration, and only for stimulation with complex, naturalistic whisker motion patterns and not for stimulation with simple trapezoidal whisker motion. Thus TBI induces long-term directional changes in integrative sensory cortical layers that depend on the complexity of the incoming sensory information. The nature of these changes allow predictions as to what types of sensory processes may be affected in TBI and contribute to post-trauma sensorimotor deficits.

  12. Laparoscopic adrenal cortex

    International Nuclear Information System (INIS)

    Peyrolou, A.; Salom, A.; Harguindeguy; Taroco, L.; Ardao, G.; Broli, F. . E mail: andresssss@adinet.com.uy

    2005-01-01

    The paper presents the case of a female patient who carried an aldosterone-secreting tumor of adrenal cortex.In the analysis of diagnosis and para clinical examinations there is particular reference to the laparoscopic surgery mode of treatment.Diagnosis should be established on the basis of clinical and laboratory tests (hypopotassemia and hyperaldosteronism).Tumor topography was confirmed through CT scan, MRI and Scintiscan in left adrenal cortex.Resection was consequently made through laparoscopic surgery.The patients evolution was excellent from the surgical viewpoint,with I levels of blood pressure, potassium and aldosterone returned to normal

  13. Mouse adhalin

    DEFF Research Database (Denmark)

    Liu, L; Vachon, P H; Kuang, W

    1997-01-01

    . To analyze the biological roles of adhalin, we cloned the mouse adhalin cDNA, raised peptide-specific antibodies to its cytoplasmic domain, and examined its expression and localization in vivo and in vitro. The mouse adhalin sequence was 80% identical to that of human, rabbit, and hamster. Adhalin...... was specifically expressed in striated muscle cells and their immediate precursors, and absent in many other cell types. Adhalin expression in embryonic mouse muscle was coincident with primary myogenesis. Its expression was found to be up-regulated at mRNA and protein levels during myogenic differentiation...

  14. Assembly of a Full-Scale External Tank Barrel Section Using Friction Stir Welding

    Science.gov (United States)

    Jones, Chip; Adams, Glynn

    1999-01-01

    A full-scale pathfinder barrel section of the External Tank for the National Aeronautics and Space Administration (NASA) Space Transport System (Space Shuttle) has been assembled at Marshall Space Flight Center (MSFC) via a collaborative effort between NASA/MSFC and Lockheed Martin Michoud Space Systems. The barrel section is 27.5 feet in diameter and 15 feet in height. The barrel was assembled using Super-Light-Weight (SLWT), orthogrid, Al-Li 2195 panel sections and a single longeron panel. A vertical weld tool at MSFC was modified to accommodate FSW and used to assemble the barrel. These modifications included the addition of a FSW weld head and new controller hardware and software, the addition of a backing anvil and the replacement of the clamping system with individually actuated clamps. Weld process 4evelopment was initially conducted to optimize the process for the welds required for completing the assembly. The variable thickness welds in the longeron section were conducted via both two-sided welds and with the use of a retractable pin tool. The barrel assembly was completed in October 1998. Details of the vertical weld tool modifications and the assembly process are presented.

  15. Triggering and measuring bent cosmic muon tracks with the Muon Spectrometer barrel for the first time

    CERN Multimedia

    Fabio Cerutti

    During the ATLAS barrel toroid stability test, bent cosmic muon tracks were seen for the first time in the ATLAS cavern by means of the ATLAS muon spectrometer. The barrel toroid has been powered at its nominal current (20.5 thousand Amperes) and kept in steady state for more than one day during the weekend of 18-19 November (see a report on this test in the Magnet section). During this test one large sector and part of a small sector of the barrel muon spectrometer were readout and used to detect the cosmic muons tracks bent by the toroidal magnetic field. Thirteen muon stations in the feet sectors (sectors 13 and 14) have been used in this test. The muon stations are formed of Resistive Plate Chambers (RPC) that were providing the muon trigger, and Monitored Drift Tubes that were used to measure with high accuracy the muon curvature hence their momentum. The Level-1 Barrel trigger chain was based on the Barrel Middle Large chambers equipped with final production modules on both the on-detector and the o...

  16. Astringency, bitterness and color changes in dry red wines before and during oak barrel aging: An updated phenolic perspective review.

    Science.gov (United States)

    Li, Si-Yu; Duan, Chang-Qing

    2018-01-30

    To understand effects of using oak barrels on the astringency, bitterness and color of dry red wines, phenolic reactions in wines before and after barrel aging are reviewed in this paper, which has been divided into three sections. The first section includes an introduction to chemical reactivities of grape-derived phenolic compounds, a summary of the phenolic reactions that occur in dry red wines before barrel aging, and a discussion of the effects of these reactions on wine astringency, bitterness and color. The second section introduces barrel types that determine the oak barrel constituents in wines (primarily oak aldehydes and ellagitannins) and presents reactions between the oak constituents and grape-derived phenolic compounds that may modulate wine astringency, bitterness and color. The final section illustrates the chemical differences between basic oxidation and over-oxidation in wines, discusses oxygen consumption kinetics in wines during barrel aging by comparing different oxygen consumption kinetics observed previously by others, and speculates on the possible preliminary phenolic reactions that occur in dry red wines during oak barrel aging that soften tannins and stabilize pigments via basic oxidation. Additionally, sulfur dioxide (SO 2 ) addition during barrel aging and suitability of adopting oak barrels for aging wines are briefly discussed.

  17. [Neuroanatomy of Frontal Association Cortex].

    Science.gov (United States)

    Takada, Masahiko

    2016-11-01

    The frontal association cortex is composed of the prefrontal cortex and the motor-related areas except the primary motor cortex (i.e., the so-called higher motor areas), and is well-developed in primates, including humans. The prefrontal cortex receives and integrates large bits of diverse information from the parietal, temporal, and occipital association cortical areas (termed the posterior association cortex), and paralimbic association cortical areas. This information is then transmitted to the primary motor cortex via multiple motor-related areas. Given these facts, it is likely that the prefrontal cortex exerts executive functions for behavioral control. The functional input pathways from the posterior and paralimbic association cortical areas to the prefrontal cortex are classified primarily into six groups. Cognitive signals derived from the prefrontal cortex are conveyed to the rostral motor-related areas to transform them into motor signals, which finally enter the primary motor cortex via the caudal motor-related areas. Furthermore, it has been shown that, similar to the primary motor cortex, areas of the frontal association cortex form individual networks (known as "loop circuits") with the basal ganglia and cerebellum via the thalamus, and hence are extensively involved in the expression and control of behavioral actions.

  18. A scintillating fibre detector for the Crystal Barrel experiment at ELSA

    International Nuclear Information System (INIS)

    Suft, G.; Anton, G.; Bogendoerfer, R.; Ehmanns, A.; Foesel, A.; Hoessl, J.; Kalinowsky, H.; Kueppersbusch, C.; Walther, D.

    2005-01-01

    A scintillating fibre detector with high spatial granularity was built for the Crystal Barrel experiment at ELSA (CB-ELSA) in Bonn. It consists of 513 scintillating fibres with 2mm in diameter, arranged in three layers with cylindrical geometry inside the Crystal Barrel detector surrounding the target cell. Two layers are wound in opposite directions, the third is parallel to the incident beam direction, resulting in an unambiguous hit reconstruction and a position resolution better than 1.6mm for charged particles. The read-out is done with 16-channel multi-anode photomultipliers. The detector was designed to cover the full angular acceptance of the Crystal Barrel detector with an angular range of 12 deg. ≤θ = 168 deg. and 0 deg. ≤φ≤360 deg. in the lab frame

  19. Analysis of core and core barrel heat-up under conditions simulating severe reactor accidents

    International Nuclear Information System (INIS)

    Chellaiah, S.; Viskanta, R.; Ranganathan, P.; Anand, N.K.

    1987-01-01

    This paper reports on the development of a model for estimating the temperature distributions in the reactor core, core barrel, thermal shield and reactor pressure vessel of a PWR during an undercooling transient. A number of numerical calculations simulating the core uncovering of the TMI-2 reactor and the subsequent heat-up of the core have been performed. The results of the calculations show that the exothermic heat release due to Zircaloy oxidation contributes to the sharp heat-up of the core. However, the core barrel temperature rise which is driven by the temperature increase of the edge of the core (e.g., the core baffle) is very modest. The maximum temperature of the core barrel never exceeded 610 K (at a system pressure of 68 bar) after a 75 minute simulation following the start of core uncovering

  20. CHANGES IN VOLATILE COMPOSITION AND SENSORY PROPERTIES OF VUGAVA WINES AGED IN CROATIA OAK BARRELS

    Directory of Open Access Journals (Sweden)

    Stanka HERJAVEC

    2001-09-01

    Full Text Available Vugava musts were fermented in medium-toasted Croatian barrique barrels (225 L made from Quercus petrea and Q. robur oak wood. The oak species used in this research infl uenced the specifi c change of the aroma structure of Vugava wines. During the age period the increase in the concentration of cis and trans oaklactons, guaiacol, eugenol, furfural and 5-methylfurfural was noted. Wines fermented and aged in Q. petrea barrels have higher concentrations of most volatile phenols compared to wines from Q. robur oak wood. From the organoleptic point of view this study suggested that fermentation and on the lees ageing production method in Croatian oak barrels positively infl uenced the quality of Vugava wines where best results were achieved by use of Q. petrea oak wood.

  1. Early stages in the biogenesis of eukaryotic β-barrel proteins.

    Science.gov (United States)

    Jores, Tobias; Rapaport, Doron

    2017-09-01

    The endosymbiotic organelles mitochondria and chloroplasts harbour, similarly to their prokaryotic progenitors, β-barrel proteins in their outer membrane. These proteins are encoded on nuclear DNA, translated on cytosolic ribosomes and imported into their target organelles by a dedicated machinery. Recent studies have provided insights into the import into the organelles and the membrane insertion of these proteins. Although the cytosolic stages of their biogenesis are less well defined, it is speculated that upon their synthesis, chaperones prevent β-barrel proteins from aggregation and keep them in an import-competent conformation. In this Review, we summarize the current knowledge about the biogenesis of β-barrel proteins, focusing on the early stages from the translation on cytosolic ribosomes to the recognition on the surface of the organelle. © 2017 Federation of European Biochemical Societies.

  2. Performance of the ATLAS Level-1 muon barrel trigger during the Run 2 data taking

    CERN Document Server

    AUTHOR|(INSPIRE)INSPIRE-00404546; The ATLAS collaboration

    2018-01-01

    The Level-1 Muon Barrel Trigger is one of the main elements of the event selection of the ATLAS experiment at the Large Hadron Collider. It exploits the Resistive Plate Chambers (RPC) detectors to generate the trigger signal. The RPCs are placed in the barrel region of the ATLAS experiment: they are arranged in three concentric double layers and operate in a strong magnetic toroidal field. RPC detectors cover the pseudo-rapidity range |η| < 1.05 for a total surface of more than 4000 m 2 and about 3600 gas volumes. The Level-1 Muon Trigger in the barrel region allows to select muon candidates according to their transverse momentum and associates them with the correct bunch-crossing. The trigger system is able to take a decision within a latency of about 2 μs. The measurement of the RPC detector efficiencies and the trigger performance during the ATLAS Run-II data taking are here presented.

  3. Distributed Wireless Monitoring System for Ullage and Temperature in Wine Barrels

    Science.gov (United States)

    Zhang, Wenqi; Skouroumounis, George K.; Monro, Tanya M.; Taylor, Dennis K.

    2015-01-01

    This paper presents a multipurpose and low cost sensor for the simultaneous monitoring of temperature and ullage of wine in barrels in two of the most important stages of winemaking, that being fermentation and maturation. The distributed sensor subsystem is imbedded within the bung of the barrel and runs on battery for a period of at least 12 months and costs around $27 AUD for all parts. In addition, software was designed which allows for the remote transmission and easy visual interpretation of the data for the winemaker. Early warning signals can be sent when the temperature or ullage deviates from a winemakers expectations so remedial action can be taken, such as when topping is required or the movement of the barrels to a cooler cellar location. Such knowledge of a wine’s properties or storage conditions allows for a more precise control of the final wine quality. PMID:26266410

  4. Development of the SSTL-300-S1 Composite Imager Barrel Structure

    Science.gov (United States)

    Hamar, Chris; Wood, Trevor; Alsami, Sami; Hallett, Ben

    2014-06-01

    The SSTL-300-S1 is the latest in the family of highly capable SSTL-300 platforms, providing high resolution imagery with all the existing mission performance of the heritage platform. In developing the product, SSTL has had to undertake the development of a composite imager barrel assembly, which forms the payload instrument's primary structure. Working to a nominal schedule of 24 months from requirements definition to structural qualification, the barrel's development philosophy has had to carefully balance the interdependent optical, structural and programmatic requirements. This paper provides a brief summary description of that development.

  5. ATLAS Level-1 Muon Barrel Trigger robustness study at X5 test facility

    CERN Document Server

    Di Mattia, A; Nisati, A; Pastore, F C; Vari, R; Veneziano, Stefano; Aielli, G; Camarri, P; Cardarelli, R; Di Ciaccio, A; Di Simone, A; Liberti, B; Santonico, R

    2004-01-01

    The present paper describes the Level-1 Barrel Muon Trigger performance as expected with the current configuration of the RPC detectors, as designed for the Barrel Muon Spectrometer of ATLAS. Results of a beam test performed at the X5-GIF facility at CERN are presented in order to show the trigger efficiency with different conditions of RPC detection efficiency and several background rates. Small RPC chambers with part of the final trigger electronics are used, while the trigger coincidence logic is applied off-line using a detailed simulation model. copy 2003 Published by Esevier B.V. 3 Refs.

  6. Application of advanced thermal management technologies to the ATLAS SCT barrel module baseboards

    Energy Technology Data Exchange (ETDEWEB)

    Apsimon, R.J. [Rutherford Appleton Laboratory, Chilton, Didcot, Oxfordshire OX11 OQX (United Kingdom); Batchelor, L.E. [Rutherford Appleton Laboratory, Chilton, Didcot, Oxfordshire OX11 OQX (United Kingdom); Beck, G.A. [Department of Physics, Queen Mary University of London, Mile End Road, London E1 4NS (United Kingdom); Canard, P. [European Laboratory for Particle Physics (CERN), 1211 Geneva 23 (Switzerland); Carter, A.A. [Department of Physics, Queen Mary University of London, Mile End Road, London E1 4NS (United Kingdom)]. E-mail: a.a.carter@qmul.ac.uk; Carter, J.R. [Cavendish Laboratory, University of Cambridge, J.J. Thomson Avenue, Cambridge CB3 0HE (United Kingdom); Davis, V.R. [Rutherford Appleton Laboratory, Chilton, Didcot, Oxfordshire OX11 OQX (United Kingdom); Oliveira, R. de [European Laboratory for Particle Physics (CERN), 1211 Geneva 23 (Switzerland); Gibson, M.D. [Rutherford Appleton Laboratory, Chilton, Didcot, Oxfordshire OX11 OQX (United Kingdom); Hominal, L. [European Laboratory for Particle Physics (CERN), 1211 Geneva 23 (Switzerland); Ilie, D.M. [Department of Physics, Queen Mary University of London, Mile End Road, London E1 4NS (United Kingdom); Ilie, S.D. [European Laboratory for Particle Physics (CERN), 1211 Geneva 23 (Switzerland); Leboube, C.G. [European Laboratory for Particle Physics (CERN), 1211 Geneva 23 (Switzerland); Mistry, J. [Department of Physics, Queen Mary University of London, Mile End Road, London E1 4NS (United Kingdom); Morin, J. [Department of Physics, Queen Mary University of London, Mile End Road, London E1 4NS (United Kingdom); Morris, J.; Nagai, K. [Department of Physics, Queen Mary University of London, Mile End Road, London E1 4NS (United Kingdom); Sexton, I.; Thery, X. [European Laboratory for Particle Physics (CERN), 1211 Geneva 23 (Switzerland); Tyndel, M. [Rutherford Appleton Laboratory, Chilton, Didcot, Oxfordshire OX11 OQX (United Kingdom)

    2006-09-15

    The paper describes the application of advanced thermal management technologies to the design and production of the barrel module baseboard of the SemiConductor Tracker (SCT) of the ATLAS experiment at the Large Hadron Collider (LHC). The barrel modules contain silicon microstrip sensors and readout ASICs for tracking charged particles, and the baseboard forms the central element of the module, providing both its necessary thermal management and its mechanical structure. The baseboard requirements and specifications are given, and design and fabrication details are described. The properties of the 3000 baseboards successfully produced for the SCT are summarised.

  7. A study of aging effects of barrel Time-Of-Flight system in the BESIII experiment

    Science.gov (United States)

    Liu, Huan-Huan; Sun, Sheng-Sen; Fang, Shuang-Shi; Wu, Zhi; Dai, Hong-Liang; Heng, Yue-Kun; Zhou, Ming; Deng, Zi-Yan; Liu, Huai-Min

    2018-02-01

    The Time-Of-Flight system consisting of plastic scintillation counters plays an important role for particle identification in the BESIII experiment at the BEPCII double ring e+e- collider. Degradation of the detection efficiency of the barrel TOF system has been observed since the start of physical data taking and this effect has triggered intensive and systematic studies about aging effects of the detector. The aging rates of the attenuation lengths and relative gains are obtained based on the data acquired in past several years. This study is essential for ensuring an extended operation of the barrel TOF system in optimal conditions.

  8. 27 CFR 25.160 - Tax adjustment for brewers who produce more than 2,000,000 barrels of beer.

    Science.gov (United States)

    2010-04-01

    ... who produce more than 2,000,000 barrels of beer. 25.160 Section 25.160 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS BEER Tax on Beer Determination of Tax § 25.160 Tax adjustment for brewers who produce more than 2,000,000 barrels of beer. Each...

  9. Start of Final Assembly of the CMS Barrel Yoke on schedule at P5 the 1st august, 2000. I

    CERN Multimedia

    Hubert Gerwig, CERN/ EP-CMI

    2000-01-01

    The Barrel Yoke and the vacuumtank weigh 6500 tonnes. The barrel Yoke consists of 5 rings eacu one weighing 1200 tonnes. The vacuum tank is a stainless steel structure weighing 270 tonnes (length = 13 m , outer diamter 7.6 m) The final assembly of these items will take approximately 1 year from now on.

  10. Involvement and necessity of the Cpx regulon in the event of aberrant β-barrel outer membrane protein assembly

    Science.gov (United States)

    Gerken, Henri; Leiser, Owen P.; Bennion, Drew; Misra, Rajeev

    2010-01-01

    Summary The Cpx and σE regulons help maintain outer membrane integrity; the Cpx pathway monitors the biogenesis of cell surface structures, such as pili, while the σE pathway monitors the biogenesis of β-barrel outer membrane proteins (OMPs). In this study we revealed the importance of the Cpx regulon in the event of β-barrel OMP mis-assembly, by utilizing mutants expressing either a defective β-barrel OMP assembly machinery (Bam) or assembly defective β-barrel OMPs. Analysis of specific mRNAs showed that ΔcpxR bam double mutants failed to induce degP expression beyond the wild type level, despite activation of the σE pathway. The synthetic conditional lethal phenotype of ΔcpxR in mutant Bam or β-barrel OMP backgrounds was reversed by wild type DegP expressed from a heterologous plasmid promoter. Consistent with the involvement of the Cpx regulon in the event of aberrant β-barrel OMP assembly, the expression of cpxP, the archetypal member of the cpx regulon, was upregulated in defective Bam backgrounds or in cells expressing a single assembly-defective β-barrel OMP species. Together, these results showed that both the Cpx and σE regulons are required to reduce envelope stress caused by aberrant β-barrel OMP assembly, with the Cpx regulon principally contributing by controlling degP expression. PMID:20487295

  11. Performance of the SLD Barrel CRID during the 1992 physics data run

    International Nuclear Information System (INIS)

    Abe, K.; Hasegawa, K.; Hasegawa, Y.; Iwasaki, Y.; Suekane, F.; Toge, N.; Yuta, H.; Baird, K.; Jacques, P.; Kalelkar, M.; Plano, R.; Stamer, P.; Word, G.; Bean, A.; Caldwell, D.O.; Duboscq, J.; Huber, J.; Lu, A.; Mathys, L.; McHugh, S.; Yellin, S.; Ben-David, R.; Manly, S.; Snyder, J.; Turk, J.; Cavalli-Sforza, M.; Colye, P.; Coyne, D.; Liu, X.; Williams, D.A.; Coller, J.; Shank, J.T.; Whitaker, J.S.; d'Oliveira, A.; Johnson, R.A.; Martinez, J.; Meadows, B.; Nussbaum, M.; Santha, A.K.S.; Sokoloff, M.D.; Stockdale, I.; Wilson, R.J.

    1992-11-01

    The SLD Barrel Cherenkov Ring Imaging Detector was fully operational in the 1992 physics data run. The electron drift velocity and magnetic field deflection of electron trajectories have been measured. Cherenkov rings have been observed from both the liquid and gas radiators. The number and the resolution of the angle of Cherenkov photons have been measured to be approximately equal to design specifications

  12. Construction and Performance of the ATLAS SCT Barrels and Cosmic Tests

    CERN Document Server

    Demirkoz, Bilge Melahat

    2007-01-01

    ATLAS is a multi-purpose detector for the LHC and will detect proton-proton collisions with center of mass energy of $14$TeV. Part of the central inner detector, the Semi-Conductor Tracker (SCT) barrels, were assembled and tested at Oxford University and later integrated at CERN with the TRT (Transition Radiation Tracker) barrel. The barrel SCT is composed of 4 layers of silicon strip modules with two sensor layers with $80 \\mu$m channel width. The design of the modules and the barrels has been optimized for low radiation length while maintaining mechanical stability, bringing services to the detector, and ensuring a cold and dry environment. The high granularity, high detector efficiency and low noise occupancy ($ < 5 \\times 10^{-4}$) of the SCT will enable ATLAS to have an efficient pattern recognition capability. Due to the binary nature of the SCT read-out, a stable read-out system and the calibration system is of critical importance. SctRodDaq is the online software framework for the calibration and a...

  13. Construction and test of the final CMS Barrel Drift Tube Muon Chamber prototype

    Energy Technology Data Exchange (ETDEWEB)

    Aguilar-Benitez, M.; Alberdi, J.; Arneodo, M.; Banicz, K.; Benettoni, M.; Benvenuti, A.; Bethke, S.; Cerrada, M. E-mail: cerrada@ciemat.es; Cirio, R.; Colino, N.; Conti, E.; Dallavalle, M.; Daniel, M.; Dattola, D.; Daudo, F.; De Giorgi, M.; Dosselli, U.; Fanfani, A.; Fanin, C.; Fouz, M.C.; Gasparini, F.; Gasparini, U.; Giacomelli, P.; Giordano, V.; Gonella, F.; Grandi, C.; Guaita, P.; Guerzoni, M.; Lacaprara, S.; Lippi, I.; Marcellini, S.; Marin, J.; Martinelli, R.; Maselli, S.; Meneguzzo, A.; Migliore, E.; Mocholi, J.; Monaco, V.; Montanari, A.; Montanari, C.; Odorici, F.; Oller, J.C.; Paoletti, S.; Passaseo, M.; Pegoraro, M.; Peroni, C.; Puerta, J.; Reithler, H.; Romero, A.; Romero, L.; Ronchese, P.; Rossi, A.M.; Rovelli, T.; Sacchi, R.; Salicio, J.M.; Staiano, A.; Steinbeck, T.; Torassa, E.; Travaglini, R.; Ventura, L.; Ventura, S.; Vitelli, A.; Voetee, F.; Wegner, M.; Willmott, C.; Zotto, P.; Zumerle, G

    2002-03-21

    A prototype of the CMS Barrel Muon Detector incorporating all the features of the final chambers was built using the mass production assembly procedures and tools. The performance of this prototype was studied in a muon test beam at CERN and the results obtained are presented in this paper.

  14. Phenolic Composition and Related Properties of Aged Wine Spirits: Influence of Barrel Characteristics. A Review

    Directory of Open Access Journals (Sweden)

    Sara Canas

    2017-11-01

    Full Text Available The freshly distilled wine spirit has a high concentration of ethanol and many volatile compounds, but is devoid of phenolic compounds other than volatile phenols. Therefore, an ageing period in the wooden barrel is required to attain sensory fullness and high quality. During this process, several phenomena take place, namely the release of low molecular weight phenolic compounds and tannins from the wood into the wine spirit. Research conducted over the last decades shows that they play a decisive role on the physicochemical characteristics and relevant sensory properties of the beverage. Their contribution to the antioxidant activity has also been emphasized. Besides, some studies show the modulating effect of the ageing technology, involving different factors such as the barrel features (including the wood botanical species, those imparted by the cooperage technology, and the barrel size, the cellar conditions, and the operations performed, on the phenolic composition and related properties of the aged wine spirit. This review aims to summarize the main findings on this topic, taking into account two featured barrel characteristics—the botanical species of the wood and the toasting level.

  15. Inference of core barrel motion from neutron noise spectral density. [PWR

    Energy Technology Data Exchange (ETDEWEB)

    Robinson, J.C.; Shahrokhi, F.; Kryter, R.C.

    1977-03-15

    A method was developed for inference of core barrel motion from the following statistical descriptors: cross-power spectral density, autopower spectral density, and amplitude probability density. To quantify the core barrel motion in a typical pressurized water reactor (PWR), a scale factor was calculated in both one- and two-dimensional geometries using forward, variational, and perturbation methods of discrete ordinates neutron transport. A procedure for selection of the proper frequency band limits for the statistical descriptors was developed. It was found that although perturbation theory is adequate for the calculation of the scale factor, two-dimensional geometric effects are important enough to rule out the use of a one-dimensional approximation for all but the crudest calculations. It was also found that contributions of gamma rays can be ignored and that the results are relatively insensitive to the cross-section set employed. The proper frequency band for the statistical descriptors is conveniently determined from the coherence and phase information from two ex-core power range neutron monitors positioned diametrically across the reactor vessel. Core barrel motion can then be quantified from the integral of the band-limited cross-power spectral density of two diametrically opposed ex-core monitors or, if the coherence between the pair is greater than or equal to 0.7, from a properly band-limited amplitude probability density function. Wide-band amplitude probability density functions were demonstrated to yield erroneous estimates for the magnitude of core barrel motion.

  16. Cosmic tests and performance of the ATLAS SemiConductor Tracker Barrels

    International Nuclear Information System (INIS)

    Demirkoez, Bilge M.

    2007-01-01

    ATLAS is a multi-purpose particle detector for the LHC and will detect proton collisions with center of mass energy of 14TeV. Part of the central inner detector, the SemiConductor Tracker (SCT) barrel, is now fully integrated with the Transition Radiation Tracker (TRT) barrel. The SCT module performance has been measured after module production, after macro-assembly of modules onto barrels, after arrival at CERN and again partially after integration with the TRT. The module noise average per channel has been stable and is 4.5x10 -5 , well below the design specification of 5x10 -4 . There is no evidence for common mode noise problems and 99.8% of the 3.2 million channels of the SCT barrels are functional. The cosmics running of the SCT and TRT was the first large scale test of the physics mode of the SCT online software framework. A large sector, 468 SCT modules, has been timed in and read out during the cosmic tests. Tracks have been reconstructed through the SCT and the TRT sectors. Present residuals from tracks (without alignment) are better than the specified building tolerances of the SCT

  17. Tob38, a novel essential component in the biogenesis of β-barrel proteins of mitochondria

    Science.gov (United States)

    Waizenegger, Thomas; Habib, Shukry J; Lech, Maciej; Mokranjac, Dejana; Paschen, Stefan A; Hell, Kai; Neupert, Walter; Rapaport, Doron

    2004-01-01

    Insertion of β-barrel proteins into the outer membrane of mitochondria is mediated by the TOB complex. Known constituents of this complex are Tob55 and Mas37. We identified a novel component, Tob38. It is essential for viability of yeast and the function of the TOB complex. Tob38 is exposed on the surface of the mitochondrial outer membrane. It interacts with Mas37 and Tob55 and is associated with Tob55 even in the absence of Mas37. The Tob38–Tob55 core complex binds precursors of β-barrel proteins and facilitates their insertion into the outer membrane. Depletion of Tob38 results in strongly reduced levels of Tob55 and Mas37 and the residual proteins no longer form a complex. Tob38-depleted mitochondria are deficient in the import of β-barrel precursor proteins, but not of other outer membrane proteins or proteins of other mitochondrial subcompartments. We conclude that Tob38 has a crucial function in the biogenesis of β-barrel proteins of mitochondria. PMID:15205677

  18. Experimental study of the single electron response of the DELPHI Barrel RICH MWPC's

    International Nuclear Information System (INIS)

    Dracos, M.

    1989-01-01

    We present herewith the results of an experimental study of the multiwire proportionnal chambers (MWPC's) used in the Barrel RICH particle identifier of the LEP-DELPHI experiment. Emphasis is given to the problem of detection efficiency and to the measurements of the charge induced by a single photoelectron

  19. Dependence of Oak-Related Volatile Compounds on the Physicochemical Characteristics of Barrel-Aged Wines

    Directory of Open Access Journals (Sweden)

    Pedro Rodríguez-Rodríguez

    2012-01-01

    Full Text Available This paper focuses on the effect of some of the physicochemical characteristics of wines such as volatile acidity, titratable acidity, pH, free SO2 and alcohol content on the accumulation of oak-related volatile compounds in barrel-aged wines, in order to give more light on the contradictory results found by other authors in this respect. For this, three different single variety wines were aged for twelve months in barrels with the same characteristics (same cooperage, wood origin, toasting level and volume, repeating the experiment in two consecutive years. Our results show that the percentage of wine alcohol and its titratable acidity positively correlated with the final concentration of vanillin and guaiacyl compounds in the oak-matured wines and negatively with the cis- and trans-β-methyl-γ-octalactone concentration. Therefore, when studying the effect of oak barrel variables (oak origin and seasoning, size of the barrel, number of uses, etc. on the concentration of oak-related volatile compounds in wine, the effect of the physicochemical variables of the wine, especially titratable acidity and alcohol content, should also be taken into account since the final wine aroma composition will also depend on these characteristics.

  20. 33 CFR 135.103 - Levy and payment of barrel fee on OCS oil.

    Science.gov (United States)

    2010-07-01

    ... SECURITY (CONTINUED) MARINE POLLUTION FINANCIAL RESPONSIBILITY AND COMPENSATION OFFSHORE OIL POLLUTION COMPENSATION FUND Levy of Fees § 135.103 Levy and payment of barrel fee on OCS oil. (a) A fee of $.03 per... paragraph (a) of this section applies whenever the unobligated Fund balance is less than $200,000,000. (d...

  1. Test of the wire ageing induced by radiation for the CMS barrel muon chamber

    CERN Document Server

    Conti, Enrico

    2000-01-01

    We have carried out laboratory test to measure the ageing of a wire tube due to pollutant outgassed by various materials. The tested materials are those used in the muon barrel drift tubes. An X-ray gun irradiated the test tube to accelerate the ageing process. No ageing effect has been measured for a period equivalent to 10 years of operation at LHC.

  2. The anterior cingulate cortex

    Directory of Open Access Journals (Sweden)

    Pavlović D.M.

    2009-01-01

    Full Text Available The anterior cingulate cortex (ACC has a role in attention, analysis of sensory information, error recognition, problem solving, detection of novelty, behavior, emotions, social relations, cognitive control, and regulation of visceral functions. This area is active whenever the individual feels some emotions, solves a problem, or analyzes the pros and cons of an action (if it is a right decision. Analogous areas are also found in higher mammals, especially whales, and they contain spindle neurons that enable complex social interactions. Disturbance of ACC activity is found in dementias, schizophrenia, depression, the obsessive-compulsive syndrome, and other neuropsychiatric diseases.

  3. Regulating prefrontal cortex activation

    DEFF Research Database (Denmark)

    Aznar, Susana; Klein, Anders Bue

    2013-01-01

    The prefrontal cortex (PFC) is involved in mediating important higher-order cognitive processes such as decision making, prompting thereby our actions. At the same time, PFC activation is strongly influenced by emotional reactions through its functional interaction with the amygdala...... of emotion-based actions, such as addiction and other impulse-related behaviors. In this review, we give an overview of the 5-HT2A receptor distribution (neuronal, intracellular, and anatomical) along with its functional and physiological effect on PFC activation, and how that relates to more recent findings...... of a regulatory effect of the PFC on the emotional control of our actions....

  4. Performance analysis for the CALIFA Barrel calorimeter of the R{sup 3}B experiment

    Energy Technology Data Exchange (ETDEWEB)

    Alvarez-Pol, H., E-mail: hector.alvarez@usc.es [Dpt. de Física de Partículas, Universidade de Santiago de Compostela, E-15782, Santiago de Compostela (Spain); Ashwood, N. [School of Physics and Astronomy, University of Birmingham, Edgbaston, Birmingham B15 2TT (United Kingdom); Aumann, T. [Institut für Kernphysik, Technische Universität Darmstadt, D-64289 Darmstadt (Germany); GSI Helmholtzzentrum für Schwerionenforschung GmbH, D-64291 Darmstadt (Germany); Bertini, D. [GSI Helmholtzzentrum für Schwerionenforschung GmbH, D-64291 Darmstadt (Germany); Cabanelas, P. [Dpt. de Física de Partículas, Universidade de Santiago de Compostela, E-15782, Santiago de Compostela (Spain); Casarejos, E. [Universidade de Vigo, E-36310 Vigo (Spain); Cederkall, J. [Department of Physics, Lund University, SE 221 00 Lund (Sweden); Cortina-Gil, D.; Díaz Fernández, P.; Duran, I. [Dpt. de Física de Partículas, Universidade de Santiago de Compostela, E-15782, Santiago de Compostela (Spain); Fiori, E. [ExtreMe Matter Institute EMMI and Research Division, GSI Helmholtzzentrum für Schwerionenforschung GmbH, D-64291 Darmstadt (Germany); Frankfurt Institute for Advanced Studies, D-60438 Frankfurt am Main (Germany); Galaviz, D. [Centro de Fsica Nuclear da Universidade de Lisboa, 1649-003 Lisbon (Portugal); Labiche, M. [STFC Daresbury Laboratory, Warrington WA4 4AD (United Kingdom); Nacher, E. [Instituto de Estructura de la Materia CSIC, Madrid (Spain); Pietras, B. [Dpt. de Física de Partículas, Universidade de Santiago de Compostela, E-15782, Santiago de Compostela (Spain); and others

    2014-12-11

    The CALIFA calorimeter is an advanced detector for gamma rays and light charged particles, accordingly optimized for the demanding requirements of the physics programme proposed for the R{sup 3}B facility at FAIR. The multipurpose character of CALIFA is required to fulfil challenging demands in energy resolution (5–6% at 1 MeV for gamma rays) and efficiency. Charged particles, e.g. protons of energies up to 320 MeV in the Barrel section, should also be identified with an energy resolution better to 1%. CALIFA is divided into two well-separated sections: a “Forward EndCap” and a cylindrical “Barrel” covering an angular range from 43.2° to 140.3°. The Barrel section, based on long CsI(Tl) pyramidal frustum crystals coupled to large area avalanche photodiodes (LAAPDs), attains the requested high efficiency for calorimetric purposes. The construction of the CALIFA Demonstrator, comprising 20% of the total detector, has already been initiated, and commissioning experiments are expected for 2014. The assessment of the capabilities and expected performance of the detector elements is a crucial step in their design, along with the prototypes evaluation. For this purpose, the Barrel geometry has been carefully implemented in the simulation package R3BRoot, including easily variable thicknesses of crystal wrapping and carbon fibre supports. A complete characterization of the calorimeter response (including efficiency, resolution, evaluation of energy and reconstruction losses) under different working conditions, with several physics cases selected to probe the detector performance over a wide range of applications, has been undertaken. Prototypes of different sections of the CALIFA Barrel have been modeled and their responses have been evaluated and compared with the experimental results. The present paper summarizes the outcome of the simulation campaign for the entire Barrel section and for the corresponding prototypes tested at different European installations.

  5. Study on natural convection in core barrel. Experimental and numerical results for band type spacer pads

    International Nuclear Information System (INIS)

    Hayashi, Kenji; Kawamata, Nobuhiro; Kamide, Hideki

    2003-03-01

    In a fast reactor an Inter-Wrapper Flow (IWF) is one of significant phenomena for decay heat removal under natural circulation condition, when a direct reactor auxiliary cooling system (DRACS) is adopted for decay heat removal system. Cold coolant provided by dipped heat exchangers (DHX) of DRACS can penetrate into the core barrel (region between the subassemblies) and it makes natural convection int he core barrel. Such IWF will depend on a spacer pad geometry of subassemblies. Water experiment, TRIF (Test Rig for Inter-wrapper Flow), was carried out for IWF in a reactor core. The test section modeled a 1/12th sector of the core and upper plenum of reactor vessel. Experimental parameters were the spacer pad geometry and flow path geometries connecting the upper plenum and core barrel. Numerical simulation using AQUA code was also performed to confirm applicability of a simulation method. An experimental series using a button type spacer pad had been carried out. Here a band type spacer pad was examined. Temperatures at subassembly wall were measured with parameter of the flow path geometries; one was a connection pipe between the upper plenum and core barrel and the other was flow hole in core former plates between the outermost subassemblies and the core barrel. It was found that these flow paths were effective to remove heat in the core in case of the band type spacer pad. A general purpose three dimensional analysis code, AQUA, was applied to the experimental analysis. Each subassembly and inter wrapper gap region were modeled by slab mesh geometry. Pressure loss coefficient at the pacer pad was set based on the geometry. The numerical simulation results were in good agreement with measured temperature profiles in the core. (author)

  6. NMR structure of the first Ig module of mouse FGFR1

    DEFF Research Database (Denmark)

    Kiselyov, V.V.; Bock, Elisabeth Marianne; Berezin, V.

    2006-01-01

    of this module. We describe here the NMR structure of the Ig1 module of mouse FGFR1. The three-dimensional fold of the module belongs to the intermediate Ig subgroup and can be described as a beta-barrel consisting of two beta-sheets. One sheet is formed by A', G, F, C, and C', and the other by A, B, B', E...

  7. Distribution and morphology of nitridergic neurons across functional domains of the rat primary somatosensory cortex

    Directory of Open Access Journals (Sweden)

    Anaelli A Nogueira-Campos

    2012-11-01

    Full Text Available The rat primary somatosensory cortex (S1 is remarkable for its conspicuous vertical compartmentalization in barrels and septal columns, which are additionally stratified in horizontal layers. Whereas excitatory neurons from each of these compartments perform different types of processing, the role of interneurons is much less clear. Among the numerous types of GABAergic interneurons, those producing nitric oxide (NO are especially puzzling, since this gaseous messenger can modulate neural activity, synaptic plasticity and neurovascular coupling. We used a quantitative morphological approach to investigate whether nitrergic interneurons, which might therefore be considered both as NO volume diffusers and as elements of local circuitry, display features that could relate to barrel cortex architecture. In fixed brain sections, nitrergic interneurons can be revealed by histochemical processing for NADPH-diaphorase (NADPHd. Here, the dendritic arbors of nitrergic neurons from different compartments of area S1 were 3D reconstructed from serial 200-μm thick sections, using 100x objective and the Neurolucida system. Standard morphological parameters were extracted for all individual arbors and compared across columns and layers. Wedge analysis was used to compute dendritic orientation indices. Supragranular layers displayed the highest density of nitrergic neurons, whereas layer IV contained nitrergic neurons with largest soma area. The highest nitrergic neuronal density was found in septa, where dendrites were previously characterized as more extense and ramified than in barrels. Dendritic arbors were not confined to the boundaries of the column nor layer of their respective soma, being mostly double-tufted and vertically oriented, except in supragranular layers. These data strongly suggest that nitrergic interneurons adapt their morphology to the dynamics of processing performed by cortical compartments.

  8. Consumer demand for green stormwater management technology in an urban setting: The case of Chicago rain barrels

    Science.gov (United States)

    Ando, Amy W.; Freitas, Luiz P. C.

    2011-12-01

    Hydrological disruption and water pollution from urbanization can be reduced if households in urban areas adopt decentralized storm water controls. We study a citywide municipal subsidized rain-barrel program in the third biggest city in the United States, Chicago, to explore what factors influence whether households purchase this sort of green storm water management technology in an urban setting. Specifically, we regress census-tract level data on the number of rain barrels adopted in different parts of the city on socioeconomic variables, data on local flood frequency, and features of the housing stock. We find that rain-barrel purchases are not correlated with local levels of flooding, even though city residents were told by program managers that rain barrels could alleviate local flooding. Instead, rain barrels are heavily concentrated in places with high-income attitudinally green populations. We do find more rain barrels were adopted in places close to rain-barrel distribution points and near sites of hydrological information campaigns; thus, policy makers might increase green-technology adoption in areas where they can do the most good by reducing transaction costs and providing education programs to those areas. Finally, our results indicate that owner occupancy is positively correlated with green-technology adoption. Low-rise rental housing may have inefficiently low levels of adoption, such that city managers might want to develop programs to encourage storm water management investments by landlords who do not live in their own properties.

  9. Effect of oak wood barrel capacity and utilization time on phenolic and sensorial profile evolution of an Encruzado white wine.

    Science.gov (United States)

    Nunes, Paulo; Muxagata, Sara; Correia, Ana C; Nunes, Fernando M; Cosme, Fernanda; Jordão, António M

    2017-11-01

    Several studies have reported the influence of diverse winemaking technologies in white wine characteristics. However, the impact of the use of different oak wood barrel capacities and utilization time on the evolution of white wine phenolic content and sensorial characteristics are not usually considered. Thus the aim of this work was to evaluate the effect of oak wood barrel capacity and utilization time on the evolution of phenolic compounds, browning potential index and sensorial profile of an Encruzado white wine. For the 180 aging days considered, the use of new oak wood barrels induced a greater increase in global phenolic composition, including several individual compounds, such as gallic and ellagic acid, independently of the barrel capacity. Tendency for a lesser increase of the browning potential index values was detected for white wines aged in new oak wood barrels. The sensorial profile evolution, showed significant differences only for the aroma descriptors, namely for 'wood aroma' and 'aroma intensity', white wine aged in 225 L new oak wood barrels being the highest scored. The results show that, in general, the use of different capacities and utilization time of oak wood barrels used for white wine aging could play an important role in white wine quality. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  10. Quantitative/Statistical Approach to Bullet-to-Firearm Identification with Consecutively Manufactured Barrels

    Energy Technology Data Exchange (ETDEWEB)

    Peter Striupaitis; R.E. Gaensslen

    2005-01-30

    Efforts to use objective image comparison and bullet scanning technologies to distinguish bullets from consecutively manufactured handgun barrels from two manufacturers gave mixed results. The ability of a technology to reliably distinguish between matching and non-matching bullets, where the non-matching bullets were as close in pattern to the matching ones as is probably possible, would provide evidence that the distinctions could be made ''objectively'', and independently of human eyes. That evidence is identical or very close to what seems to be needed to satisfy Daubert standards. It is fair to say that the FTI IBIS image comparison technology correctly distinguished between all the Springfield barrel bullets, and between most but not all of the HiPoint barrel bullets. In the HiPoint cases that were not distinguished 100% of the time, they would he distinguished correctly at least 83% of the time. These results, although obviously limited to the materials used in the comparisons, provide strong evidence that barrel-to-bullet matching is objectively reliable. The results with SciClops were less compelling. The results do not mean that bullet-to-barrel matching is not objectively reliable--rather, they mean that this version of the particular technology could not quite distinguish between these extremely similar yet different bullets as well as the image comparison technology did. In a number of cases, the numerical results made the correct distinctions, although they were close to one another. It is hard to say from this data that this technology differs in its ability to make distinctions between the manufacturers, because the results are very similar with both. The human examiner results were as expected. We did not expect any misidentifications, and there were not any. It would have been preferable to have a higher return rate, and thus more comparisons in the overall sample. As noted, the ''consecutively manufactured barrel

  11. Cobalamin-Independent Methionine Synthase (MetE): A Face-to-Face Double Barrel that Evolved by Gene Duplication

    Energy Technology Data Exchange (ETDEWEB)

    Pejcha, Robert; Ludwig, Martha L. (Michigan)

    2010-03-08

    Cobalamin-independent methionine synthase (MetE) catalyzes the transfer of a methyl group from methyltetrahydrofolate to L-homocysteine (Hcy) without using an intermediate methyl carrier. Although MetE displays no detectable sequence homology with cobalamin-dependent methionine synthase (MetH), both enzymes require zinc for activation and binding of Hcy. Crystallographic analyses of MetE from T. maritima reveal an unusual dual-barrel structure in which the active site lies between the tops of the two ({beta}{alpha}){sub 8} barrels. The fold of the N-terminal barrel confirms that it has evolved from the C-terminal polypeptide by gene duplication; comparisons of the barrels provide an intriguing example of homologous domain evolution in which binding sites are obliterated. The C-terminal barrel incorporates the zinc ion that binds and activates Hcy. The zinc-binding site in MetE is distinguished from the (Cys){sub 3}Zn site in the related enzymes, MetH and betaine-homocysteine methyltransferase, by its position in the barrel and by the metal ligands, which are histidine, cysteine, glutamate, and cysteine in the resting form of MetE. Hcy associates at the face of the metal opposite glutamate, which moves away from the zinc in the binary E {center_dot} Hcy complex. The folate substrate is not intimately associated with the N-terminal barrel; instead, elements from both barrels contribute binding determinants in a binary complex in which the folate substrate is incorrectly oriented for methyl transfer. Atypical locations of the Hcy and folate sites in the C-terminal barrel presumably permit direct interaction of the substrates in a ternary complex. Structures of the binary substrate complexes imply that rearrangement of folate, perhaps accompanied by domain rearrangement, must occur before formation of a ternary complex that is competent for methyl transfer.

  12. Cobalamin-Independent Methionine Synthase (MetE): A Face-to-Face Double Barrel that Evolved by Gene Duplication

    International Nuclear Information System (INIS)

    Pejcha, Robert; Ludwig, Martha L.

    2005-01-01

    Cobalamin-independent methionine synthase (MetE) catalyzes the transfer of a methyl group from methyltetrahydrofolate to L-homocysteine (Hcy) without using an intermediate methyl carrier. Although MetE displays no detectable sequence homology with cobalamin-dependent methionine synthase (MetH), both enzymes require zinc for activation and binding of Hcy. Crystallographic analyses of MetE from T. maritima reveal an unusual dual-barrel structure in which the active site lies between the tops of the two (βα) 8 barrels. The fold of the N-terminal barrel confirms that it has evolved from the C-terminal polypeptide by gene duplication; comparisons of the barrels provide an intriguing example of homologous domain evolution in which binding sites are obliterated. The C-terminal barrel incorporates the zinc ion that binds and activates Hcy. The zinc-binding site in MetE is distinguished from the (Cys) 3 Zn site in the related enzymes, MetH and betaine-homocysteine methyltransferase, by its position in the barrel and by the metal ligands, which are histidine, cysteine, glutamate, and cysteine in the resting form of MetE. Hcy associates at the face of the metal opposite glutamate, which moves away from the zinc in the binary E · Hcy complex. The folate substrate is not intimately associated with the N-terminal barrel; instead, elements from both barrels contribute binding determinants in a binary complex in which the folate substrate is incorrectly oriented for methyl transfer. Atypical locations of the Hcy and folate sites in the C-terminal barrel presumably permit direct interaction of the substrates in a ternary complex. Structures of the binary substrate complexes imply that rearrangement of folate, perhaps accompanied by domain rearrangement, must occur before formation of a ternary complex that is competent for methyl transfer.

  13. Cobalamin-independent methionine synthase (MetE: a face-to-face double barrel that evolved by gene duplication.

    Directory of Open Access Journals (Sweden)

    Robert Pejchal

    2005-02-01

    Full Text Available Cobalamin-independent methionine synthase (MetE catalyzes the transfer of a methyl group from methyltetrahydrofolate to L-homocysteine (Hcy without using an intermediate methyl carrier. Although MetE displays no detectable sequence homology with cobalamin-dependent methionine synthase (MetH, both enzymes require zinc for activation and binding of Hcy. Crystallographic analyses of MetE from T. maritima reveal an unusual dual-barrel structure in which the active site lies between the tops of the two (betaalpha(8 barrels. The fold of the N-terminal barrel confirms that it has evolved from the C-terminal polypeptide by gene duplication; comparisons of the barrels provide an intriguing example of homologous domain evolution in which binding sites are obliterated. The C-terminal barrel incorporates the zinc ion that binds and activates Hcy. The zinc-binding site in MetE is distinguished from the (Cys(3Zn site in the related enzymes, MetH and betaine-homocysteine methyltransferase, by its position in the barrel and by the metal ligands, which are histidine, cysteine, glutamate, and cysteine in the resting form of MetE. Hcy associates at the face of the metal opposite glutamate, which moves away from the zinc in the binary E.Hcy complex. The folate substrate is not intimately associated with the N-terminal barrel; instead, elements from both barrels contribute binding determinants in a binary complex in which the folate substrate is incorrectly oriented for methyl transfer. Atypical locations of the Hcy and folate sites in the C-terminal barrel presumably permit direct interaction of the substrates in a ternary complex. Structures of the binary substrate complexes imply that rearrangement of folate, perhaps accompanied by domain rearrangement, must occur before formation of a ternary complex that is competent for methyl transfer.

  14. Word Recognition in Auditory Cortex

    Science.gov (United States)

    DeWitt, Iain D. J.

    2013-01-01

    Although spoken word recognition is more fundamental to human communication than text recognition, knowledge of word-processing in auditory cortex is comparatively impoverished. This dissertation synthesizes current models of auditory cortex, models of cortical pattern recognition, models of single-word reading, results in phonetics and results in…

  15. Analysis of Premature Wear-Out of Aircraft Gun Barrel by Applying a Transmission Electron Microscopy (TEM

    Directory of Open Access Journals (Sweden)

    Łęczycki Krzysztof

    2017-08-01

    Full Text Available Firing from gun armament generates a wide range of physico-chemical phenomena contributing to the degradation of barrel material, e.g. pressure, high temperature, chemically aggressive character of post-detonation gases and friction of a driving band. In this work the technique of thin foils was used in Transmission Electron Microscopy (TEM to study the influence of physicochemical phenomena on the material microstructure of aircraft gun barrel. A mechanism and reason of premature wear-out of the barrel under consideration was also outlined by utilising light microscopy and hardness measurements.

  16. SiPM photosensors and fast timing readout for the Barrel Time-of-Flight detector in bar PANDA

    Science.gov (United States)

    Suzuki, K.

    2018-03-01

    The Barrel Time-of-Flight detector system will be installed in the upcoming bar PANDA experiment at FAIR in Germany. The detector has a barrel shape of phi=0.5 m and 1.8 m long, covering about 5 m2, which corresponds to the laboratory polar angle coverage of 22oPANDA Barrel Time-of-Flight detector are presented. The test shows that the current design fulfils satisfactorily the required timing performance (σt~ 56 ps) and the timing performance depends little on the hit position on the surface.

  17. Estimating induced-activation of SCT barrel-modules in the ATLAS radiation environment.

    CERN Document Server

    Buttar, C M; Dawson, I; Mandic, I; Moraes, A

    2002-01-01

    One of the consequences of operating detector systems in the harsh radiation environments of the ATLAS inner-detector will be radioactivation of the components. If the levels of radioactivity and corresponding dose rates are significant, then there will be implications for any access or maintenance operations. Given in this note are predictions for the radioactivation of SCT barrel-modules in the expected radiation environment of the inner-detector, based on both calculations and measurements. It is shown that both neutron-capture and high-energy hadron reactions must be taken into account. The predictions show that, from a radiological point of view, the SCT barrel-modules should not pose any serious problems.

  18. Current Status of the Pixel Phase I Upgrade in CMS: Barrel Module Production

    CERN Document Server

    Bartek, Rachel

    2016-01-01

    The silicon pixel detector is the innermost component of the CMS tracking system, providing high precision space point measurements of charged particle trajectories. Before 2018 the instantaneous luminosity of the LHC is expected to reach about 2~x~$10^{34}~\\rm{cm}^{-2}\\rm{s}^{-1}$, which will significantly increase the number of interactions per bunch crossing. To maintain a high tracking efficiency, CMS has planned to replace the current pixel system during phase I by a new lightweight detector, equipped with an additional 4th layer in the barrel, and one additional forward/backward disk. The present status of barrel modules production will be presented, including preliminary results from tests on the first production pixel modules of the new pixel tracker.

  19. Double-barrelled wet colostomy formation after pelvic exenteration for locally advanced or recurrent rectal cancer.

    Science.gov (United States)

    Bloemendaal, A L A; Kraus, R; Buchs, N C; Hamdy, F C; Hompes, R; Cogswell, L; Guy, R J

    2016-11-01

    In advanced pelvic cancer it may be necessary to perform a total pelvic exenteration. In such cases urinary tract reconstruction is usually achieved with the creation of an ileal conduit with a urinary stoma on the right side of the patient's abdomen and an end colostomy separately on the left. The potential morbidity from a second stoma may be avoided by the use of a double-barrelled wet colostomy (DBWC), as a single stoma. Another advantage is the possibility of using a vertical rectus abdominis muscle flap for perineal reconstruction. All patients undergoing formation of a DBWC were included. A DBWC was formed in 10 patients. One patient underwent formation of a double-barrelled wet ileostomy. In this technical note we present our early experience in 11 cases and a video of DBWC formation in a male patient. Colorectal Disease © 2016 The Association of Coloproctology of Great Britain and Ireland.

  20. Design, Construction and Installation of the ATLAS Hadronic Barrel Scintillator-Tile Calorimeter

    CERN Document Server

    Abdallah, J; Alexa, C; Alves, R; Amaral, P; Ananiev, A; Anderson, K; Andresen, X; Antonaki, A; Batusov, V; Bednar, P; Bergeaas, E; Biscarat, C; Blanch, O; Blanchot, G; Bohm, C; Boldea, V; Bosi, F; Bosman, M; Bromberg, C; Budagov, Yu A; Calvet, D; Cardeira, C; Carli, T; Carvalho, J; Cascella, M; Castillo, M V; Costello, J; Cavalli-Sforza, M; Cavasinni, V; Cerqueira, A S; Clément, C; Cobal, M; Cogswell, F; Constantinescu, S; Costanzo, D; Da Silva, P; Davidek, M; David, T; Dawson, J; De, K; Del Prete, T; Di Girolamo, B; Dita, S; Dolejsi, J; Dolezal, Z; Dotti, A; Downing, R; Drake, G; Efthymiopoulos, I; Errede, D; Errede, S; Farbin, A; Fassouliotis, D; Feng, E; Fenyuk, A; Ferdi, C; Ferreira, B C; Ferrer, A; Flaminio, V; Flix, J; Francavilla, P; Fullana, E; Garde, V; Gellerstedt, K; Giakoumopoulou, V; Giangiobbe, V; Gildemeister, O; Gilewsky, V; Giokaris, N; Gollub, N; Gomes, A; González, V; Gouveia, J; Grenier, P; Gris, P; Guarino, V; Guicheney, C; Sen-Gupta, A; Hakobyan, H; Haney, M; Hellman, S; Henriques, A; Higón, E; Hill, N; Holmgren, S; Hruska, I; Hurwitz, M; Huston, J; Jen-La Plante, I; Jon-And, K; Junk, T; Karyukhin, A; Khubua, J; Klereborn, J; Kopikov, S; Korolkov, I; Krivkova, P; Kulchitsky, Y; Kurochkin, Yu; Kuzhir, P; Lapin, V; Le Compte, T; Lefèvre, R; Leitner, R; Li, J; Liablin, M; Lokajícek, M; Lomakin, Y; Lourtie, P; Lovas, L; Lupi, A; Maidantchik, C; Maio, A; Maliukov, S; Manousakis, A; Marques, C; Marroquim, F; Martin, F; Mazzoni, E; Merritt, F S; Myagkov, A; Miller, R; Minashvili, I; Miralles, L; Montarou, G; Némécek, S; Nessi, M; Nikitine, I; Nodulman, L; Norniella, O; Onofre, A; Oreglia, M; Palan, B; Pallin, D; Pantea, D; Pereira, A; Pilcher, J E; Pina, J; Pinhão, J; Pod, E; Podlyski, F; Portell, X; Poveda, J; Pribyl, L; Price, L E; Proudfoot, J; Ramalho, M; Ramstedt, M; Raposeiro, L; Reis, J; Richards, R; Roda, C; Romanov, V; Rosnet, P; Roy, P; Ruiz, A; Rumiantsau, V; Russakovich, N; Sada Costa, J; Salto, O; Salvachúa, B; Sanchis, E; Sanders, H; Santoni, C; Santos, J; Saraiva, J G; Sarri, F; Says, L P; Schlager, G; Schlereth, J L; Seixas, J M; Selldén, B; Shalanda, N; Shevtsov, P; Shochet, M; Simaitis, V; Simonyan, M; Sisakian, A; Sjölin, J; Solans, C; Solodkov, A; Solovianov, J; Silva, O; Sosebee, M; Spanó, F; Speckmeyer, P; Stanek, R; Starchenko, E; Starovoitov, P; Suk, M; Sykora, I; Tang, F; Tas, P; Teuscher, R; Tokar, S; Topilin, N; Torres, J; Underwood, D; Usai, G; Valero, A; Valkár, S; Valls, J A; Vartapetian, A; Vazeille, F; Vellidis, C; Ventura, F; Vichou, I; Vivarelli, I; Volpi, M; White, A; Zaitsev, A; Zenin, A; Zenis, T; Zenonos, Z; Zenz, S; Zilka, B

    2007-01-01

    The scintillator tile hadronic calorimeter is a sampling calorimeter using steel as the absorber structure and scintillator as the active medium. The scintillator is located in "pockets" in the steel structure and the wavelength-shifting fibers are contained in channels running radially within the absorber to photomultiplier tubes which are located in the outer support girders of the calorimeter structure. In addition, to its role as a detector for high energy particles, the tile calorimeter provides the direct support of the liquid argon electromagnetic calorimeter in the barrel region, and the liquid argon electromagnetic and hadronic calorimeters in the endcap region. Through these, it indirectly supports the inner tracking system and beam pipe. The steel absorber, and in particular the support girders, provide the flux return for the solenoidal field from the central solenoid. Finally, the end surfaces of the barrel calorimeter are used to mount services, power supplies and readout crates for the inner tr...

  1. A Barrel IFR Instrumented With Limited Streamer Tubes for BABAR Experiment

    International Nuclear Information System (INIS)

    Andreotti, M.; Ferrara U.; INFN, Ferrara

    2006-01-01

    The new barrel Instrumented Flux Return (IFR) of BABAR detector will be reported here. Limited Streamer Tubes (LSTs) have been chosen to replace the existing RPCs as active elements of the barrel IFR. The layout of the new detector will be discussed: in particular, a cell bigger than the standard one has been used to improve efficiency and reliability. The extruded profile is coated with a resistive layer of graphite having a typical surface resistivity between 0.2 and 0.4 MOhm/square. The tubes are assembled in modules and installed in 12 active layers of each sextant of the IFR detector. R and D studies to choose the final design and Quality Control procedure adopted during the tube production will be briefly discussed. Finally the performances of installed LSTs into 2/3 of IFR after 8 months of operations will be reported

  2. Intercalibration of the barrel electromagnetic calorimeter of the CMS experiment at start-up

    CERN Document Server

    Adzic, Petar; Almeida, Nuno; Anagnostou, Georgios; Andelin, Daniel; Anfreville, Marc; Anicin, Ivan; Antunovic, Zeljko; Arcidiacono, Roberta; Arenton, Michael Wayne; Auffray, Etiennette; Argiro, Stefano; Askew, Andrew; Atramentov, Oleksiy; Baccaro, Stefania; Baffioni, Stephanie; Balazs, Michael; Barney, David; Barone, Luciano; Bartoloni, Alessandro; Baty, Clement; Bandurin, Dmitry; Beauceron, Stephanie; Bell, Ken W; Benetta, Robert; Bercher, Michel; Bernet, Colin; Berthon, Ursula; Besançon, Marc; Betev, Botjo; Beuselinck, Raymond; Biino, Cristina; Blaha, Jan; Bloch, Philippe; Blyth, Simon; Bornheim, Adolf; Bourotte, Jean; Brett, Angela Mary; Brown, Robert M; Britton, David; Bühler, M; Busson, Philippe; Camanzi, Barbara; Camporesi, Tiziano; Carrera, E; Cartiglia, Nicolo; Cavallari, Francesca; Cerutti, Muriel; Chang, Paoti; Chang, You-Hao; Charlot, Claude; Chen, E Augustine; Chen, Wan-Ting; Chen, Zheng-Yu; Chipaux, Rémi; Choudhary, Brajesh C; Choudhury, Rajani Kant; Cockerill, David J A; Combaret, Christophe; Conetti, Sergio; Cossutti, Fabio; Cox, Bradley; Cussans, David; Dafinei, Ioan; Da Silva Di Calafiori, Diogo Raphael; Daskalakis, Georgios; Davatz, Giovanna; David, A; Deiters, Konrad; Dejardin, Marc; Djordjevic, Milos; Della Negra, Rodolphe; Della Ricca, Giuseppe; Del Re, Daniele; De Min, Alberto; Denegri, Daniel; Depasse, Pierre; Descamps, Julien; Diemoz, Marcella; Di Marco, Emanuele; Dissertori, Günther; Dittmar, Michael; Djambazov, Lubomir; Dobrzynski, Ludwik; Drndarevic, Snezana; Duboscq, Jean Etienne; Dutta, Dipanwita; Dzelalija, Mile; Peisert, A; El-Mamouni, H; Evangelou, Ioannis; Evans, David; Fabbro, Bernard; Faure, Jean-Louis; Fay, Jean; Ferri, Federico; Flower, Paul S; Franci, Daniele; Franzoni, Giovanni; Freudenreich, Klaus; Funk, Wolfgang; Ganjour, Serguei; Gargiulo, Corrado; Gascon, Susan; Gataullin, Marat; Geerebaert, Yannick; Gentit, François-Xavier; Gershtein, Yuri; Ghezzi, Alessio; Ghodgaonkar, Manohar; Gilly, Jean; Givernaud, Alain; Gninenko, Sergei; Go, Apollo; Gobbo, Benigno; Godinovic, Nikola; Golubev, Nikolai; Gong, Datao; Govoni, Pietro; Grant, Nicholas; Gras, Philippe; Greenhalgh, R J S; Guevara Riveros, Luz; Guillaud, Jean-Paul; Haguenauer, Maurice; Hamel de Monchenault, Gautier; Hansen, Magnus; Heath, Helen F; Heltsley, Brian; Hill, Jack; Hintz, Wieland; Hirosky, Robert; Hobson, Peter R; Honma, Alan; Hou, George Wei-Shu; Hsiung, Yee; Husejko, Michal; Ille, Bernard; Imlay, Richard; Ingram, Quentin; Jarry, Patrick; Jessop, Colin; Jovanovic, Dragoslav; Kaadze, Ketino; Kachanov, Vassili; Kailas, Swaminathan; Kataria, Sushil Kumar; Kennedy, Bruce W; Kloukinas, Kostas; Kokkas, Panagiotis; Kolberg, Ted; Krasnikov, Nikolai; Krpic, Dragomir; Kubota, Yuichi; Kumar, P; Kuo, Chen-Cheng; Kyberd, Paul; Kyriakis, Aristotelis; Lebeau, Michel; Lecomte, Pierre; Lecoq, Paul; Ledovskoy, Alexander; Leshev, Georgi; Lethuillier, Morgan; Lin, Sheng-Wen; Lin, Willis; Lintern, A L; Litvine, Vladimir; Locci, Elizabeth; Lodge, Anthony B; Longo, Egidio; Loukas, Demetrios; Luckey, Paul David; Lustermann, Werner; Lynch, Clare; Ma, Yousi; Mahlke-Krüger, H; Malberti, Martina; Malcles, Julie; Maletic, Dimitrije; Mandjavidze, Irakli; Manthos, Nikolaos; Maravin, Yurii; Marchica, Carmelo; Marinelli, Nancy; Markou, Athanasios; Markou, Christos; Marone, Matteo; Mathez, Hervé; Matveev, Viktor; Mavrommatis, Charalampos; Maurelli, Georges; Meridiani, Paolo; Milenovic, Predrag; Milleret, Gérard; Miné, Philippe; Mohanty, Ajit Kumar; Moortgat, Filip; Mur, Michel; Musella, Pasquale; Musienko, Yuri; Nardulli, Alessandro; Nash, Jordan; Nédélec, Patrick; Negri, Pietro; Newman, Harvey B; Nikitenko, Alexander; Nessi-Tedaldi, Francesca; Obertino, Maria Margherita; Organtini, Giovanni; Orimoto, Toyoko; Paganoni, Marco; Paganini, Pascal; Palma, Alessandro; Panev, Bozhidar; Pant, Lalit Mohan; Papadakis, Antonakis; Papadakis, Ioannis; Papadopoulos, Ioannis; Paramatti, Riccardo; Parracho, P; Pastrone, Nadia; Patterson, Juliet Ritchie; Pauss, Felicitas; Petrakou, Eleni; Phillips, D G; Piroué, Pierre; Ptochos, Fotios; Puljak, Ivica; Pullia, Antonino; Punz, Thomas; Puzovic, Jovan; Ragazzi, Stefano; Rahatlou, Shahram; Rander, John; Razis, Panos A; Redaelli, Nicola; Renker, Dieter; Reucroft, Steve; Reymond, Jean-Marc; Ribeiro, Pedro Quinaz; Röser, Ulf; Rogan, Christopher; Romanteau, Thierry; Rondeaux, Françoise; Ronquest, Michael; Rosowsky, André; Rovelli, Chiara; Rumerio, Paolo; Rusack, Roger; Rusakov, Sergey V; Ryan, Matthew John; Sala, Leonardo; Salerno, Roberto; Santanastasio, Francesco; Schinzel, Dietrich; Seez, Christopher; Sharp, Peter; Shepherd-Themistocleous, Claire; Siamitros, Christos; Sillou, Daniel; Singovsky, Alexander; Sirois, Yves; Sirunyan, Albert M; Silva, J; Silva, Pedro; Shiu, Jing-Ge; Shivpuri, Ram Krishen; Shukla, Prashant; Smith, Brian; Smith, Vincent J; Sproston, Martin; Stöckli, Fabian; Suter, Henry; Swain, John; Tabarellide Fatis, T; Takahashi, Maiko; Tcheremoukhine, Alexandre; Teller, Olivier; Theofilatos, Konstantinos; Thiebaux, Christophe; Timciuc, Vladlen; Timlin, Claire; Titov, Maksym; Tobias, A; Topkar, Anita; Triantis, Frixos A; Troshin, Sergey; Tyurin, Nikolay; Ueno, Koji; Uzunian, Andrey; Varela, Joao; Verrecchia, Patrice; Veverka, Jan; Vichoudis, Paschalis; Virdee, Tejinder; Vlassov, E; Von Gunten, Hans Peter; Wang, Minzu; Wardrope, David; Weber, Markus; Weng, Joanna; Williams, Jennifer C; Yang, Yong; Yaselli, Ignacio; Yohay, Rachel; Zabi, Alexandre; Zamiatin, Nikolai; Zelepoukine, Serguei; Zhang, Jia-Wen; Zhang, Lin; Zhu, Kejun; Zhu, Ren-Yuan

    2008-01-01

    Calibration of the relative response of the individual channels of the barrel electromagnetic calorimeter of the CMS detector was accomplished before installation with cosmic ray muons and test beams. One fourth of the calorimeter was exposed to a beam of high energy electrons and the relative calibration of the channels, the intercalibration, was found to be reproducible to a precision of about 0.3\\%. Additionally, data were collected with cosmic rays for the entire ECAL barrel during the commissioning phase. By comparing the intercalibration constants obtained with the electron beam data with those from the cosmic ray data, it is demonstrated that the latter provide an intercalibration precision of 1.5\\% over most of the ECAL. The best intercalibration precision is expected to come from the analysis of events collected {\\it in situ} during the LHC operation. Using data collected with both electrons and pion beams, several aspects of the intercalibration procedures based on electrons or neutral pions were in...

  3. TEM study of radiation induced defects in baffle-former-barrel assembly from decommissioned NPP Greifswald

    International Nuclear Information System (INIS)

    Srba, O.; Michalicka, J.; Keilova, E.; Kocik, K.

    2013-06-01

    A complex transmission electron microscopy (TEM) study of reactor vessel internal (RVI) materials from the baffle-former-barrel assembly from NPP Greifswald (VVER 440), Unit 1 decommissioned after 15 service cycles has been undertaken. All parts of the baffle-former-barrel assembly are made from Ti-stabilized austenitic stainless steel 08Ch18N10T. The materials were exposed to different dose of neutron radiation (2.4 - 11.4 dpa) at temperatures 267 - 398 deg. C depending on position in the core. Three types of radiation induced defects were identified and quantified, namely: dislocations, cavities (voids) and fine-scaled precipitated particles of Ni-Si rich phases. Black-dot type defects were observed too. Operation conditions are around ≅ 300 deg. C that is why we have observed defect typical for both low and high regions of irradiation temperatures. (authors)

  4. The CMS Barrel Calorimeter Response to Particle Beams from 2 to 350 GeV/c

    CERN Document Server

    Yazgan, Elfe

    2009-01-01

    The response of the combined CMS barrel calorimeters to hadrons, electrons and muons over a range from 2 to 350 GeV/$c$ has been measured. The analysis of the differences in calorimeter response to charged pions, kaons, protons and antiprotons and a discussion of the underlying phenomena are presented. Techniques to correct the signals from the considerably different electromagnetic (EB) and hadronic (HB) barrel calorimeters in reconstructing the energies of hadrons are also presented. Above 5 GeV/$c$, these corrections improve the energy resolution of the combined system where the stochastic term equals $84.7$\\% and the constant term is $7.4$\\%. The corrected mean response remains constant within 1.3\\% {\\it rms}.

  5. Study of a twisted ATLAS SCT Barrel deformation as revealed by a photogrammetric survey

    CERN Document Server

    Dobson, E; Heinemann, F; Karagoz-Unel, M

    2007-01-01

    A photogrammetry survey on the SCT barrels was performed as an engineering check on the structure of the ATLAS Semiconductor Tracker (SCT) shortly after construction. Analysis of the data obtained revealed small scale elliptical deformation as well as a twist of the structure. The results of the survey are presented as well as interpolation of the measured targets to the module positions and a comparison with track based alignment measurements.

  6. Diagnosis of vibrations of barrel and fuel elements in the PWRs of Relishing Sweden

    International Nuclear Information System (INIS)

    Pazsit, I.; Martin, M.

    2011-01-01

    The diagnosis of the inside of the PWR, in particular the core barrel and its support can be performed by analysis of the signals from the neutron detectors extra-nuclear. Several methods have been developed that have been used in various plants worldwide. The objective is to monitor the integrity of the core structure and the early detection and quantification of signs of fatigue, wear, etc. in the different structures.

  7. Performance Study of the CMS Barrel Resistive Plate Chambers with Cosmic Rays

    CERN Document Server

    Chatrchyan, S; Sirunyan, A M; Adam, W; Arnold, B; Bergauer, H; Bergauer, T; Dragicevic, M; Eichberger, M; Erö, J; Friedl, M; Frühwirth, R; Ghete, V M; Hammer, J; Hänsel, S; Hoch, M; Hörmann, N; Hrubec, J; Jeitler, M; Kasieczka, G; Kastner, K; Krammer, M; Liko, D; Magrans de Abril, I; Mikulec, I; Mittermayr, F; Neuherz, B; Oberegger, M; Padrta, M; Pernicka, M; Rohringer, H; Schmid, S; Schöfbeck, R; Schreiner, T; Stark, R; Steininger, H; Strauss, J; Taurok, A; Teischinger, F; Themel, T; Uhl, D; Wagner, P; Waltenberger, W; Walzel, G; Widl, E; Wulz, C E; Chekhovsky, V; Dvornikov, O; Emeliantchik, I; Litomin, A; Makarenko, V; Marfin, I; Mossolov, V; Shumeiko, N; Solin, A; Stefanovitch, R; Suarez Gonzalez, J; Tikhonov, A; Fedorov, A; Karneyeu, A; Korzhik, M; Panov, V; Zuyeuski, R; Kuchinsky, P; Beaumont, W; Benucci, L; Cardaci, M; De Wolf, E A; Delmeire, E; Druzhkin, D; Hashemi, M; Janssen, X; Maes, T; Mucibello, L; Ochesanu, S; Rougny, R; Selvaggi, M; Van Haevermaet, H; Van Mechelen, P; Van Remortel, N; Adler, V; Beauceron, S; Blyweert, S; D'Hondt, J; De Weirdt, S; Devroede, O; Heyninck, J; Kalogeropoulos, A; Maes, J; Maes, M; Mozer, M U; Tavernier, S; Van Doninck, W; Van Mulders, P; Villella, I; Bouhali, O; Chabert, E C; Charaf, O; Clerbaux, B; De Lentdecker, G; Dero, V; Elgammal, S; Gay, A P R; Hammad, G H; Marage, P E; Rugovac, S; Vander Velde, C; Vanlaer, P; Wickens, J; Grunewald, M; Klein, B; Marinov, A; Ryckbosch, D; Thyssen, F; Tytgat, M; Vanelderen, L; Verwilligen, P; Basegmez, S; Bruno, G; Caudron, J; Delaere, C; Demin, P; Favart, D; Giammanco, A; Grégoire, G; Lemaitre, V; Militaru, O; Ovyn, S; Piotrzkowski, K; Quertenmont, L; Schul, N; Beliy, N; Daubie, E; Alves, G A; Pol, M E; Souza, M H G; Carvalho, W; De Jesus Damiao, D; De Oliveira Martins, C; Fonseca De Souza, S; Mundim, L; Oguri, V; Santoro, A; Silva Do Amaral, S M; Sznajder, A; Fernandez Perez Tomei, T R; Ferreira Dias, M A; Gregores, E M; Novaes, S F; Abadjiev, K; Anguelov, T; Damgov, J; Darmenov, N; Dimitrov, L; Genchev, V; Iaydjiev, P; Piperov, S; Stoykova, S; Sultanov, G; Trayanov, R; Vankov, I; Dimitrov, A; Dyulendarova, M; Kozhuharov, V; Litov, L; Marinova, E; Mateev, M; Pavlov, B; Petkov, P; Toteva, Z; Chen, G M; Chen, H S; Guan, W; Jiang, C H; Liang, D; Liu, B; Meng, X; Tao, J; Wang, J; Wang, Z; Xue, Z; Zhang, Z; Ban, Y; Cai, J; Ge, Y; Guo, S; Hu, Z; Mao, Y; Qian, S J; Teng, H; Zhu, B; Avila, C; Baquero Ruiz, M; Carrillo Montoya, C A; Gomez, A; Gomez Moreno, B; Ocampo Rios, A A; Osorio Oliveros, A F; Reyes Romero, D; Sanabria, J C; Godinovic, N; Lelas, K; Plestina, R; Polic, D; Puljak, I; Antunovic, Z; Dzelalija, M; Brigljevic, V; Duric, S; Kadija, K; Morovic, S; Fereos, R; Galanti, M; Mousa, J; Papadakis, A; Ptochos, F; Razis, P A; Tsiakkouri, D; Zinonos, Z; Hektor, A; Kadastik, M; Kannike, K; Müntel, M; Raidal, M; Rebane, L; Anttila, E; Czellar, S; Härkönen, J; Heikkinen, A; Karimäki, V; Kinnunen, R; Klem, J; Kortelainen, M J; Lampén, T; Lassila-Perini, K; Lehti, S; Lindén, T; Luukka, P; Mäenpää, T; Nysten, J; Tuominen, E; Tuominiemi, J; Ungaro, D; Wendland, L; Banzuzi, K; Korpela, A; Tuuva, T; Nedelec, P; Sillou, D; Besancon, M; Chipaux, R; Dejardin, M; Denegri, D; Descamps, J; Fabbro, B; Faure, J L; Ferri, F; Ganjour, S; Gentit, F X; Givernaud, A; Gras, P; Hamel de Monchenault, G; Jarry, P; Lemaire, M C; Locci, E; Malcles, J; Marionneau, M; Millischer, L; Rander, J; Rosowsky, A; Rousseau, D; Titov, M; Verrecchia, P; Baffioni, S; Bianchini, L; Bluj, M; Busson, P; Charlot, C; Dobrzynski, L; Granier de Cassagnac, R; Haguenauer, M; Miné, P; Paganini, P; Sirois, Y; Thiebaux, C; Zabi, A; Agram, J L; Besson, A; Bloch, D; Bodin, D; Brom, J M; Conte, E; Drouhin, F; Fontaine, J C; Gelé, D; Goerlach, U; Gross, L; Juillot, P; Le Bihan, A C; Patois, Y; Speck, J; Van Hove, P; Baty, C; Bedjidian, M; Blaha, J; Boudoul, G; Brun, H; Chanon, N; Chierici, R; Contardo, D; Depasse, P; Dupasquier, T; El Mamouni, H; Fassi, F; Fay, J; Gascon, S; Ille, B; Kurca, T; Le Grand, T; Lethuillier, M; Lumb, N; Mirabito, L; Perries, S; Vander Donckt, M; Verdier, P; Djaoshvili, N; Roinishvili, N; Roinishvili, V; Amaglobeli, N; Adolphi, R; Anagnostou, G; Brauer, R; Braunschweig, W; Edelhoff, M; Esser, H; Feld, L; Karpinski, W; Khomich, A; Klein, K; Mohr, N; Ostaptchouk, A; Pandoulas, D; Pierschel, G; Raupach, F; Schael, S; Schultz von Dratzig, A; Schwering, G; Sprenger, D; Thomas, M; Weber, M; Wittmer, B; Wlochal, M; Actis, O; Altenhöfer, G; Bender, W; Biallass, P; Erdmann, M; Fetchenhauer, G; Frangenheim, J; Hebbeker, T; Hilgers, G; Hinzmann, A; Hoepfner, K; Hof, C; Kirsch, M; Klimkovich, T; Kreuzer, P; Lanske, D; Merschmeyer, M; Meyer, A; Philipps, B; Pieta, H; Reithler, H; Schmitz, S A; Sonnenschein, L; Sowa, M; Steggemann, J; Szczesny, H; Teyssier, D; Zeidler, C; Bontenackels, M; Davids, M; Duda, M; Flügge, G; Geenen, H; Giffels, M; Haj Ahmad, W; Hermanns, T; Heydhausen, D; Kalinin, S; Kress, T; Linn, A; Nowack, A; Perchalla, L; Poettgens, M; Pooth, O; Sauerland, P; Stahl, A; Tornier, D; Zoeller, M H; Aldaya Martin, M; Behrens, U; Borras, K; Campbell, A; Castro, E; Dammann, D; Eckerlin, G; Flossdorf, A; Flucke, G; Geiser, A; Hatton, D; Hauk, J; Jung, H; Kasemann, M; Katkov, I; Kleinwort, C; Kluge, H; Knutsson, A; Kuznetsova, E; Lange, W; Lohmann, W; Mankel, R; Marienfeld, M; Meyer, A B; Miglioranzi, S; Mnich, J; Ohlerich, M; Olzem, J; Parenti, A; Rosemann, C; Schmidt, R; Schoerner-Sadenius, T; Volyanskyy, D; Wissing, C; Zeuner, W D; Autermann, C; Bechtel, F; Draeger, J; Eckstein, D; Gebbert, U; Kaschube, K; Kaussen, G; Klanner, R; Mura, B; Naumann-Emme, S; Nowak, F; Pein, U; Sander, C; Schleper, P; Schum, T; Stadie, H; Steinbrück, G; Thomsen, J; Wolf, R; Bauer, J; Blüm, P; Buege, V; Cakir, A; Chwalek, T; De Boer, W; Dierlamm, A; Dirkes, G; Feindt, M; Felzmann, U; Frey, M; Furgeri, A; Gruschke, J; Hackstein, C; Hartmann, F; Heier, S; Heinrich, M; Held, H; Hirschbuehl, D; Hoffmann, K H; Honc, S; Jung, C; Kuhr, T; Liamsuwan, T; Martschei, D; Mueller, S; Müller, Th; Neuland, M B; Niegel, M; Oberst, O; Oehler, A; Ott, J; Peiffer, T; Piparo, D; Quast, G; Rabbertz, K; Ratnikov, F; Ratnikova, N; Renz, M; Saout, C; Sartisohn, G; Scheurer, A; Schieferdecker, P; Schilling, F P; Schott, G; Simonis, H J; Stober, F M; Sturm, P; Troendle, D; Trunov, A; Wagner, W; Wagner-Kuhr, J; Zeise, M; Zhukov, V; Ziebarth, E B; Daskalakis, G; Geralis, T; Karafasoulis, K; Kyriakis, A; Loukas, D; Markou, A; Markou, C; Mavrommatis, C; Petrakou, E; Zachariadou, A; Gouskos, L; Katsas, P; Panagiotou, A; Evangelou, I; Kokkas, P; Manthos, N; Papadopoulos, I; Patras, V; Triantis, F A; Bencze, G; Boldizsar, L; Debreczeni, G; Hajdu, C; Hernath, S; Hidas, P; Horvath, D; Krajczar, K; Laszlo, A; Patay, G; Sikler, F; Toth, N; Vesztergombi, G; Beni, N; Christian, G; Imrek, J; Molnar, J; Novak, D; Palinkas, J; Szekely, G; Szillasi, Z; Tokesi, K; Veszpremi, V; Kapusi, A; Marian, G; Raics, P; Szabo, Z; Trocsanyi, Z L; Ujvari, B; Zilizi, G; Bansal, S; Bawa, H S; Beri, S B; Bhatnagar, V; Jindal, M; Kaur, M; 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Yang, Y; Zhang, L; Zhu, K; Zhu, R Y; Akgun, B; Carroll, R; Ferguson, T; Jang, D W; Jun, S Y; Paulini, M; Russ, J; Terentyev, N; Vogel, H; Vorobiev, I; Cumalat, J P; Dinardo, M E; Drell, B R; Ford, W T; Heyburn, B; Luiggi Lopez, E; Nauenberg, U; Stenson, K; Ulmer, K; Wagner, S R; Zang, S L; Agostino, L; Alexander, J; Blekman, F; Cassel, D; Chatterjee, A; Das, S; Gibbons, L K; Heltsley, B; Hopkins, W; Khukhunaishvili, A; Kreis, B; Kuznetsov, V; Patterson, J R; Puigh, D; Ryd, A; Shi, X; Stroiney, S; Sun, W; Teo, W D; Thom, J; Vaughan, J; Weng, Y; Wittich, P; Beetz, C P; Cirino, G; Sanzeni, C; Winn, D; Abdullin, S; Afaq, M A; Albrow, M; Ananthan, B; Apollinari, G; Atac, M; Badgett, W; Bagby, L; Bakken, J A; Baldin, B; Banerjee, S; Banicz, K; Bauerdick, L A T; Beretvas, A; Berryhill, J; Bhat, P C; Biery, K; Binkley, M; Bloch, I; Borcherding, F; Brett, A M; Burkett, K; Butler, J N; Chetluru, V; Cheung, H W K; Chlebana, F; Churin, I; Cihangir, S; Crawford, M; Dagenhart, W; Demarteau, M; Derylo, G; Dykstra, D; Eartly, D P; Elias, J E; Elvira, V D; Evans, D; Feng, L; Fischler, M; Fisk, I; Foulkes, S; Freeman, J; Gartung, P; Gottschalk, E; Grassi, T; Green, D; Guo, Y; Gutsche, O; Hahn, A; Hanlon, J; Harris, R M; Holzman, B; Howell, J; Hufnagel, D; James, E; Jensen, H; Johnson, M; Jones, C D; Joshi, U; Juska, E; Kaiser, J; Klima, B; Kossiakov, S; Kousouris, K; Kwan, S; Lei, C M; Limon, P; Lopez Perez, J A; Los, S; Lueking, L; Lukhanin, G; Lusin, S; Lykken, J; Maeshima, K; Marraffino, J M; Mason, D; McBride, P; Miao, T; Mishra, K; Moccia, S; Mommsen, R; Mrenna, S; Muhammad, A S; Newman-Holmes, C; Noeding, C; O'Dell, V; Prokofyev, O; Rivera, R; Rivetta, C H; Ronzhin, A; Rossman, P; Ryu, S; Sekhri, V; Sexton-Kennedy, E; Sfiligoi, I; Sharma, S; Shaw, T M; Shpakov, D; Skup, E; Smith, R P; Soha, A; Spalding, W J; Spiegel, L; Suzuki, I; Tan, P; Tanenbaum, W; Tkaczyk, S; Trentadue, R; Uplegger, L; Vaandering, E W; Vidal, R; Whitmore, J; Wicklund, E; Wu, W; Yarba, J; Yumiceva, F; Yun, J C; Acosta, D; Avery, P; Barashko, V; Bourilkov, D; Chen, M; Di Giovanni, G P; Dobur, D; Drozdetskiy, A; Field, R D; Fu, Y; Furic, I K; Gartner, J; Holmes, D; Kim, B; Klimenko, S; Konigsberg, J; Korytov, A; Kotov, K; Kropivnitskaya, A; Kypreos, T; Madorsky, A; Matchev, K; Mitselmakher, G; Pakhotin, Y; Piedra Gomez, J; Prescott, C; Rapsevicius, V; Remington, R; Schmitt, M; Scurlock, B; Wang, D; Yelton, J; Ceron, C; Gaultney, V; Kramer, L; Lebolo, L M; Linn, S; Markowitz, P; Martinez, G; Rodriguez, J L; Adams, T; Askew, A; Baer, H; Bertoldi, M; Chen, J; Dharmaratna, W G D; Gleyzer, S V; Haas, J; Hagopian, S; Hagopian, V; Jenkins, M; Johnson, K F; Prettner, E; Prosper, H; Sekmen, S; Baarmand, M M; Guragain, S; Hohlmann, M; Kalakhety, H; Mermerkaya, H; Ralich, R; Vodopiyanov, I; Abelev, B; Adams, M R; Anghel, I M; Apanasevich, L; Bazterra, V E; Betts, R R; Callner, J; Castro, M A; Cavanaugh, R; Dragoiu, C; Garcia-Solis, E J; Gerber, C E; Hofman, D J; Khalatian, S; Mironov, C; Shabalina, E; Smoron, A; Varelas, N; Akgun, U; Albayrak, E A; Ayan, A S; Bilki, B; Briggs, R; Cankocak, K; Chung, K; Clarida, W; Debbins, P; Duru, F; Ingram, F D; Lae, C K; McCliment, E; Merlo, J P; Mestvirishvili, A; Miller, M J; Moeller, A; Nachtman, J; Newsom, C R; Norbeck, E; Olson, J; Onel, Y; Ozok, F; Parsons, J; Schmidt, I; Sen, S; Wetzel, J; Yetkin, T; Yi, K; Barnett, B A; Blumenfeld, B; Bonato, A; Chien, C Y; Fehling, D; Giurgiu, G; Gritsan, A V; Guo, Z J; Maksimovic, P; Rappoccio, S; Swartz, M; Tran, N V; Zhang, Y; Baringer, P; Bean, A; Grachov, O; Murray, M; Radicci, V; Sanders, S; Wood, J S; Zhukova, V; Bandurin, D; Bolton, T; Kaadze, K; Liu, A; Maravin, Y; Onoprienko, D; Svintradze, I; Wan, Z; Gronberg, J; Hollar, J; Lange, D; Wright, D; Baden, D; Bard, R; Boutemeur, M; Eno, S C; Ferencek, D; Hadley, N J; Kellogg, R G; Kirn, M; Kunori, S; Rossato, K; Rumerio, P; Santanastasio, F; Skuja, A; Temple, J; Tonjes, M B; Tonwar, S C; Toole, T; Twedt, E; Alver, B; Bauer, G; Bendavid, J; Busza, W; Butz, E; Cali, I A; Chan, M; D'Enterria, D; Everaerts, P; Gomez Ceballos, G; Hahn, K A; Harris, P; Jaditz, S; Kim, Y; Klute, M; Lee, Y J; Li, W; Loizides, C; Ma, T; Miller, M; Nahn, S; Paus, C; Roland, C; Roland, G; Rudolph, M; Stephans, G; Sumorok, K; Sung, K; Vaurynovich, S; Wenger, E A; Wyslouch, B; Xie, S; Yilmaz, Y; Yoon, A S; Bailleux, D; Cooper, S I; Cushman, P; Dahmes, B; De Benedetti, A; Dolgopolov, A; Dudero, P R; Egeland, R; Franzoni, G; Haupt, J; Inyakin, A; Klapoetke, K; Kubota, Y; Mans, J; Mirman, N; Petyt, D; Rekovic, V; Rusack, R; Schroeder, M; Singovsky, A; Zhang, J; Cremaldi, L M; Godang, R; Kroeger, R; Perera, L; Rahmat, R; Sanders, D A; Sonnek, P; Summers, D; Bloom, K; Bockelman, B; Bose, S; Butt, J; Claes, D R; Dominguez, A; Eads, M; Keller, J; Kelly, T; Kravchenko, I; Lazo-Flores, J; Lundstedt, C; Malbouisson, H; Malik, S; Snow, G R; Baur, U; Iashvili, I; Kharchilava, A; Kumar, A; Smith, K; Strang, M; Alverson, G; Barberis, E; Boeriu, O; Eulisse, G; Govi, G; McCauley, T; Musienko, Y; Muzaffar, S; Osborne, I; Paul, T; Reucroft, S; Swain, J; Taylor, L; Tuura, L; Anastassov, A; Gobbi, B; Kubik, A; Ofierzynski, R A; Pozdnyakov, A; Schmitt, M; Stoynev, S; Velasco, M; Won, S; Antonelli, L; Berry, D; Hildreth, M; Jessop, C; Karmgard, D J; Kolberg, T; Lannon, K; Lynch, S; Marinelli, N; Morse, D M; Ruchti, R; Slaunwhite, J; Warchol, J; Wayne, M; Bylsma, B; Durkin, L S; Gilmore, J; Gu, J; Killewald, P; Ling, T Y; Williams, G; Adam, N; Berry, E; Elmer, P; Garmash, A; Gerbaudo, D; Halyo, V; Hunt, A; Jones, J; Laird, E; Marlow, D; Medvedeva, T; Mooney, M; Olsen, J; Piroué, P; Stickland, D; Tully, C; Werner, J S; Wildish, T; Xie, Z; Zuranski, A; Acosta, J G; Bonnett Del Alamo, M; Huang, X T; Lopez, A; Mendez, H; Oliveros, S; Ramirez Vargas, J E; Santacruz, N; Zatzerklyany, A; Alagoz, E; Antillon, E; Barnes, V E; Bolla, G; Bortoletto, D; Everett, A; Garfinkel, A F; Gecse, Z; Gutay, L; Ippolito, N; Jones, M; Koybasi, O; Laasanen, A T; Leonardo, N; Liu, C; Maroussov, V; Merkel, P; Miller, D H; Neumeister, N; Sedov, A; Shipsey, I; Yoo, H D; Zheng, Y; Jindal, P; Parashar, N; Cuplov, V; Ecklund, K M; Geurts, F J M; Liu, J H; Maronde, D; Matveev, M; Padley, B P; Redjimi, R; Roberts, J; Sabbatini, L; Tumanov, A; Betchart, B; Bodek, A; Budd, H; Chung, Y S; de Barbaro, P; Demina, R; Flacher, H; Gotra, Y; Harel, A; Korjenevski, S; Miner, D C; Orbaker, D; Petrillo, G; Vishnevskiy, D; Zielinski, M; Bhatti, A; Demortier, L; Goulianos, K; Hatakeyama, K; Lungu, G; Mesropian, C; Yan, M; Atramentov, O; Bartz, E; Gershtein, Y; Halkiadakis, E; Hits, D; Lath, A; Rose, K; Schnetzer, S; Somalwar, S; Stone, R; Thomas, S; Watts, T L; Cerizza, G; Hollingsworth, M; Spanier, S; Yang, Z C; York, A; Asaadi, J; Aurisano, A; Eusebi, R; Golyash, A; Gurrola, A; Kamon, T; Nguyen, C N; Pivarski, J; Safonov, A; Sengupta, S; Toback, D; Weinberger, M; Akchurin, N; Berntzon, L; Gumus, K; Jeong, C; Kim, H; Lee, S W; Popescu, S; Roh, Y; Sill, A; Volobouev, I; Washington, E; Wigmans, R; Yazgan, E; Engh, D; Florez, C; Johns, W; Pathak, S; Sheldon, P; Andelin, D; Arenton, M W; Balazs, M; Boutle, S; Buehler, M; Conetti, S; Cox, B; Hirosky, R; Ledovskoy, A; Neu, C; Phillips II, D; Ronquest, M; Yohay, R; Gollapinni, S; Gunthoti, K; Harr, R; Karchin, P E; Mattson, M; Sakharov, A; Anderson, M; Bachtis, M; Bellinger, J N; Carlsmith, D; Crotty, I; Dasu, S; Dutta, S; Efron, J; Feyzi, F; Flood, K; Gray, L; Grogg, K S; Grothe, M; Hall-Wilton, R; Jaworski, M; Klabbers, P; Klukas, J; Lanaro, A; Lazaridis, C; Leonard, J; Loveless, R; Magrans de Abril, M; Mohapatra, A; Ott, G; Polese, G; Reeder, D; Savin, A; Smith, W H; Sourkov, A; Swanson, J; Weinberg, M; Wenman, D; Wensveen, M; White, A

    2010-01-01

    In October and November 2008, the CMS collaboration conducted a programme of cosmic ray data taking, which has recorded about 270 million events. The Resistive Plate Chamber system, which is part of the CMS muon detection system, was successfully operated in the full barrel. More than 98% of the channels were operational during the exercise with typical detection efficiency of 90%. In this paper, the performance of the detector during these dedicated runs is reported.

  8. Influence of fuel vibration on PWR neutron noise associated with core barrel motion

    International Nuclear Information System (INIS)

    Sweeney, F.J.; March-Leuba, J.

    1984-01-01

    Ex-core neutron detector noise has been utilized to monitor core support barrel (CSB) vibrations. In order to observe long-term changes, noise signals at Sequoyah-1 were monitored continuously during the whole first fuel cycle and part of the second cycle. Results suggest that neutron noise measurements performed infrequently may not provide adequate surveillance of the CSB because it may be difficult to separate noise amplitude changes due solely to CSB motion from changes caused by fuel motion and burnup

  9. Homemade battery-operated multi-barreled muzzle-loading gun.

    Science.gov (United States)

    Ramiah, R; Thirunavukkarasu, G

    2003-11-01

    In a recent shootout by a terrorist group against a law enforcement agency, some unusual firearms were seized. On examination, these firearms were found to be homemade, battery-operated, multi-barreled muzzle-loading guns, analogous to a repeater. Reference to battery-operated firearms is rather scanty in the literature. Hence, the unique design features, electrical circuit, and the operation system of these unusual guns are described.

  10. Distortion of the temporary cavity and its influence on staining in firearm barrels.

    Science.gov (United States)

    Schyma, Christian; Müller, Rolf; Brenčičová, Eva; Brünig, Julia

    2018-06-01

    After contact shots to the head, biological traces can be found inside the barrel of the firearm. Experimental protocols to generate this sort of staining, using 12 cm gelatin cubes containing thin foil bags filled with acrylic paint, human blood, and radiocontrast agent, have been developed. Previous research on shots fired at a distance has shown the underlay sustaining these gelatin cubes has an influence on experimental results. This study was conducted to investigate the role of the sustaining base of the gelatin blocks during contact shots, and its influence on the staining result inside firearm barrels. Eighteen contact shots were performed using 22 LR, 32 ACP (7.65 Browning) and 9 mm Luger semi-automatic pistols. With each pistol, shots were fired onto six gelatin cubes; three placed upon a rigid platform and three upon an elastic underlay. The shots were recorded by a high-speed video camera as they penetrated the gelatin cube. Any staining present inside the firearm barrels after the shots were fired was documented by endoscopy. Cross sections of the gelatin blocks were then compared to the high-speed video. It was found that the nature of the staining inside the barrel was not influenced by the underlay sustaining the target model. In the experiment using a 9 mm Luger, the rigid counterfort provoked a visible distortion of the temporary cavity, but, cross sectional analysis of the gelatin cubes did not reveal a relevant influence of the sustaining underlay on the crack length in the gelatin. This could be explained by a secondary expansion of the temporary cavity left by the projectile as a consequence of subsequent inflow of muzzle gases.

  11. Fluid-structure coupled dynamic response of PWR core barrel during LOCA

    International Nuclear Information System (INIS)

    Lu, M.W.; Zhang, Y.G.; Shi, F.

    1991-01-01

    This paper is engaged in the Fluid-Structure Interaction LOCA analysis of the core barrel of PWR. The analysis is performed by a multipurpose computer code SANES. The FSI inside the pressure vessel is treated by a FEM code including some structural and acoustic elements. The transient in the primary loop is solved by a two-phase flow code. Both codes are coupled one another. Some interesting conclusions are drawn. (author)

  12. Right and left support feet of the Central Barrel Yoke of the CMS Detector

    CERN Multimedia

    Franz Leher, DWE

    2000-01-01

    Fully loaded the Central Barrel will weigh 3000 tonnes. Those feet have tosupport this weight. Therefore they are made of 120 mm thick steel plates.To guarantee a maximum coverage for the muon detctor they will house a muon detector just benaeth the top plate. Weight of 1 foot is 35 tonnes.Its height is 3.5 m and it is 2.5 m large

  13. Reversibility and two state behaviour in the thermal unfolding of oligomeric TIM barrel proteins.

    Science.gov (United States)

    Romero-Romero, Sergio; Costas, Miguel; Rodríguez-Romero, Adela; Alejandro Fernández-Velasco, D

    2015-08-28

    Temperature is one of the main variables that modulate protein function and stability. Thermodynamic studies of oligomeric proteins, the dominant protein natural form, have been often hampered because irreversible aggregation and/or slow reactions are common. There are no reports on the reversible equilibrium thermal unfolding of proteins composed of (β/α)8 barrel subunits, albeit this "TIM barrel" topology is one of the most abundant and versatile in nature. We studied the eponymous TIM barrel, triosephosphate isomerase (TIM), belonging to five species of different bacterial taxa. All of them were found to be catalytically efficient dimers. The three-dimensional structure of four enzymes was solved at high/medium resolution. Irreversibility and kinetic control were observed in the thermal unfolding of two TIMs, while for the other three the thermal unfolding was found to follow a two-state equilibrium reversible process. Shifts in the global stability curves of these three proteins are related to the organismal temperature range of optimal growth and modulated by variations in maximum stability temperature and in the enthalpy change at that temperature. Reversibility appears to correlate with the low isoelectric point, the absence of a residual structure in the unfolded state, small cavity volume in the native state, low conformational stability and a low melting temperature. Furthermore, the strong coupling between dimer dissociation and monomer unfolding may reduce aggregation and favour reversibility. It is therefore very thought-provoking to find that a common topological ensemble, such as the TIM barrel, can unfold/refold in the Anfinsen way, i.e. without the help of the cellular machinery.

  14. Safety analysis of ventilation and inspection operations on barrels in which spent fuel elements are stored

    International Nuclear Information System (INIS)

    Pavlovic, R.; Todorovic, D.; Plecas, I.

    2000-01-01

    Safety analysis of possible accident during performing ventilation and inspection operations on barrels in which spent fuel elements are stored has been proposed. Investigation is confined to a case when primary contamination is localized to the disposal room and controlled release of gaseous effluents to the atmosphere. 85 Kr is the most important radionuclide considered risk estimation due to release of gaseous fission products (author) [sr

  15. Mechanical design and material budget of the CMS barrel pixel detector

    Energy Technology Data Exchange (ETDEWEB)

    Amsler, C; Boesiger, K; Chiochia, V; Maier, R; Meyer, Hp; Robmann, P; Scherr, S; Schmidt, A; Steiner, S [Universitaet Zuerich, Physik-Institut, Winterthurerstr. 190, CH-8057 Zuerich (Switzerland); Erdmann, W; Gabathuler, K; Horisberger, R; Koenig, S; Kotlinski, D; Meier, B; Streuli, S [Paul Scherrer Institut, CH-5232 Villigen (Switzerland); Rizzi, A [ETH Zuerich, Institute for Particle Physics, CH-8093 Zuerich (Switzerland)], E-mail: Alexander.Schmidt@cern.ch

    2009-05-15

    The Compact Muon Solenoid experiment at the Large Hadron Collider at CERN includes a silicon pixel detector as its innermost component. Its main task is the precise reconstruction of charged particles close to the primary interaction vertex. This paper gives an overview of the mechanical requirements and design choices for the barrel pixel detector. The distribution of material in the detector as well as its description in the Monte Carlo simulation are discussed in detail.

  16. Mechanical Design and Material Budget of the CMS Barrel Pixel Detector

    CERN Document Server

    Amsler, C; Chiochia, V; Erdmann, W; Gabathuler, K; Horisberger, R; König, S; Kotlinski, D; Maier, R; Meyer, H; Meier, B; Meyer, Hp; Rizzi, A; Robmann, P; Scherr, S; Schmidt, A; Steiner, S; Erdmann, W; Gabathuler, K; Horisberger, R; König, S; Kotlinski, D; Meier, B; Streuli, S; Rizzi, A

    2009-01-01

    The Compact Muon Solenoid experiment at the Large Hadron Collider at CERN includes a silicon pixel detector as its innermost component. Its main task is the precise reconstruction of charged particles close to the primary interaction vertex. This paper gives an overview of the mechanical requirements and design choices for the barrel pixel detector. The distribution of material in the detector as well as its description in the Monte Carlo simulation are discussed in detail.

  17. Test of the wire ageing induced by radiation for the CMS barrel muon chambers

    CERN Document Server

    Conti, E

    2001-01-01

    We have carried out laboratory tests to measure the ageing of a wire tube due to pollutants outgassed by various materials. The tested materials are those used in the barrel muon drift tubes of the CMS experiment at LHC. An X-ray gun irradiated the test tube to accelerate the ageing process. No ageing effect has been measured for a period equivalent to 10 years of operation at LHC. (15 refs).

  18. Performances of the ATLAS Level-1 Muon barrel trigger during the Run-II data taking

    CERN Document Server

    Sessa, Marco; The ATLAS collaboration

    2017-01-01

    The Level-1 Muon Barrel Trigger is one of the main elements of the event selection of the ATLAS experiment at the Large Hadron Collider. It exploits the Resistive Plate Chambers (RPC) detectors to generate the trigger signal. The RPCs are placed in the barrel region of the ATLAS experiment: they are arranged in three concentric double layers and operate in a strong magnetic toroidal field. RPC detectors cover the pseudo-rapidity range $|\\eta|<1.05$ for a total surface of more than $4000\\ m^2$ and about 3600 gas volumes. The Level-1 Muon Trigger in the barrel region allows to select muon candidates with respect to their transverse momentum and associates them with the correct bunch-crossing number. The trigger system is able to take a decision within a latency of about 2 $\\mu s$. The detailed measurement of the RPC detector efficiencies and of the trigger performance during the ATLAS Run-II data taking is here presented.

  19. Forming H-shaped and barrel-shaped nebulae with interacting jets

    Science.gov (United States)

    Akashi, Muhammad; Bear, Ealeal; Soker, Noam

    2018-04-01

    We conduct three-dimensional hydrodynamical simulations of two opposite jets with large opening angles launched from a binary stellar system into a previously ejected shell and show that the interaction can form barrel-like and H-like shapes in the descendant nebula. Such features are observed in planetary nebulae (PNe) and supernova remnants. Under our assumption, the dense shell is formed by a short instability phase of the giant star as it interacts with a stellar companion, and the jets are then launched by the companion as it accretes mass through an accretion disc from the giant star. We find that the H-shaped and barrel-shaped morphological features that the jets form evolve with time, and that there are complicated flow patterns, such as vortices, instabilities, and caps moving ahead along the symmetry axis. We compare our numerical results with images of 12 PNe, and show that jet-shell interaction that we simulate can account for the barrel-like or H-like morphologies that are observed in these PNe.

  20. Upgrade of the CMS muon trigger system in the barrel region

    CERN Document Server

    Rabady, Dinyar; Carlin, Roberto; Codispoti, Giuseppe; Dallavalle, Marco; Erö, Janos; Flouris, Giannis; Foudas, Costas; Fulcher, Jonathan; Guiducci, Luigi; Loukas, Nikitas; Mallios, Stavros; Manthos, Nikos; Papadopoulos, Ioannis; Paradas, Evangelos; Reis, Thomas; Sakulin, Hannes; Sphicas, Paris; Triossi, Andrea; Venturi, Andrea; Wulz, Claudia-Elisabeth

    2016-01-01

    To maintain the excellent performance of the LHC during its Run-1 also in Run-2, the Level-1 Trigger of the Compact Muon Solenoid experiment underwent a significant upgrade. One part of this upgrade was the re-organisation of the muon trigger path from a subsystem-centric view in which hits in the drift tubes, the cathode strip chambers, and the resistive plate chambers were treated separately in dedicated track-finding systems, to one in which complementary detector systems for a given region (barrel, overlap, and endcap) are merged already at the track-finding level. This also required the development of a new system to sort as well as cancel-out the muon tracks found by each system. An overview will be given of the new track-finder system for the barrel region, the Barrel Muon Track Finder (BMTF) as well as the cancel-out and sorting layer, the upgraded Global Muon Trigger (µGMT). While the BMTF improves on the proven and well-tested algorithms used in the Drift Tube Track Finder during Run-1, the µGMT i...

  1. Upgrade of the CMS muon trigger system in the barrel region

    CERN Document Server

    Battilana, Carlo; Codispoti, Giuseppe; Dallavalle, Gaetano-Marco; Ero, Janos; Flouris, Giannis; Fountas, Konstantinos; Fulcher, Jonathan Richard; Guiducci, Luigi; Loukas, Nikitas; Mallios, Stavros; Manthos, Nikolaos; Papadopoulos, Ioannis; Paradas, Evangelos; Rabady, Dinyar Sebastian; Reis, Thomas; Sakulin, Hannes; Sphicas, Paraskevas; Triossi, Andrea; Venturi, Andrea; Wulz, Claudia

    2016-01-01

    To maintain the excellent performance of the LHC during its Run-1 also in Run-2, the Level-1 Trigger of the Compact Muon Solenoid experiment underwent a significant upgrade. One part of this upgrade was the re-organisation of the muon trigger path from a subsystem-centric view in which hits in the drift tubes, the cathode strip chambers, and the resistive plate chambers were treated separately in dedicated track-finding systems, to one in which complementary detector systems for a given region (barrel, overlap, and endcap) are merged already at the track-finding level. This also required the development of a new system to sort as well as cancel-out the muon tracks found by each system. An overview will be given of the new track-finder system for the barrel region, the Barrel Muon Track Finder (BMTF) as well as the cancel-out and sorting layer, the upgraded Global Muon Trigger ($\\mu$GMT). While the BMTF improves on the proven and well-tested algorithms used in the Drift Tube Track Finder during Run-1, the $\\m...

  2. Upgrade of the CMS muon trigger system in the barrel region

    CERN Document Server

    Rabady, Dinyar; Carlin, Roberto; Codispoti, Giuseppe; Dallavalle, Marco; Erö, Janos; Flouris, Giannis; Foudas, Costas; Fulcher, Jonathan; Guiducci, Luigi; Loukas, Nikitas; Mallios, Stavros; Manthos, Nikos; Papadopoulos, Ioannis; Paradas, Evangelos; Reis, Thomas; Sakulin, Hannes; Sphicas, Paris; Triossi, Andrea; Venturi, Andrea; Wulz, Claudia-Elisabeth

    2017-01-01

    To maintain the excellent performance of the LHC during its Run-1 also in Run-2, the Level-1 Trigger of the Compact Muon Solenoid experiment underwent a significant upgrade. One part of this upgrade was the re-organisation of the muon trigger path from a subsystem-centric view in which hits in the drift tubes, the cathode strip chambers, and the resistive plate chambers were treated separately in dedicated track-finding systems, to one in which complementary detector systems for a given region (barrel, overlap, and endcap) are merged already at the track-finding level. This also required the development of a new system to sort as well as cancel-out the muon tracks found by each system. An overview will be given of the new track-finder system for the barrel region, the Barrel Muon Track Finder (BMTF) as well as the cancel-out and sorting layer, the upgraded Global Muon Trigger (µGMT). While the BMTF improves on the proven and well-tested algorithms used in the Drift Tube Track Finder during Run-1, the µGMT i...

  3. Design studies for the Phase II upgrade of the CMS Barrel Electromagnetic Calorimeter

    CERN Document Server

    Orimoto, Toyoko Jennifer

    2016-01-01

    The High Luminosity LHC (HL-LHC) will provide unprecedented instantaneous and integrated luminosity. The lead tungstate crystals forming the barrel part of the Electromagnetic Calorimeter (ECAL) of the Compact Muon Solenoid (CMS) will still perform well, even after the expected integrated luminosity of 3000fb-1 at the end of HL-LHC. The avalanche photodiodes (APDs) used to detect the scintillation light will also continue to be operational, although there will be some increase in noise due to radiation-induced dark currents. This will be mitigated by reducing the barrel operating temperature during HL-LHC running.The front-end electronics of the ECAL barrel will be replaced, in order to remove existing constraints on trigger rate and latency and to provide additional capability to fully exploit the higher luminosity delivered by the HL-LHC. New developments in high-speed optical links will allow single-crystal readout at 40 MHz to upgraded off-detector processors, allowing maximum flexibility and enhanced tri...

  4. Upgrade of the CMS muon trigger system in the barrel region

    CERN Document Server

    AUTHOR|(CDS)2080489; Flouris, Gianis; Fulcher, Jonathan; Loukas, Nikitas; Paradas, Evangelos; Reis,Thomas; Sakulin, Hannes; Wulz, Claudia-Elisabeth

    2016-01-01

    To maintain the excellent performance shown during the LHCs Run-1 the Level-1 Trigger of the Compact Muon Solenoid experiment underwent a significant upgrade. One part of this upgrade is the re-organization of the muon trigger path from a subsystem-centric view in which hits in the drift tubes (DT), the cathode strip chambers (CSC), and the resistive plate chambers (RPC) were treated separately in dedicated track-finding systems to one in which complementary detector systems for a given region (barrel, overlap, and endcap) are merged at the track-finding level. This fundamental restructuring of the muon trigger system required the development of a system to receive track candidates from the track-finding layer, remove potential duplicate tracks, and forward the best candidates to the global decision layer.An overview will be given of the new track-finder system for the barrel region, the Barrel Muon Track Finder (BMTF) as well as the cancel-out and sorting layer, the upgraded Global Muon Trigger ($\\mu$GMT). B...

  5. Identification of Clearance and Contact Stiffness in a Simplified Barrel-Cradle Structure of Artillery System

    Directory of Open Access Journals (Sweden)

    Bing Li

    2015-02-01

    Full Text Available In gun barrel-cradle structure, the presence of clearance usually changes the dynamic response of muzzle and results in shooting dispersion (under continuous firing condition. The parameter estimation of such clearance nonlinear system is the prerequisite for establishing quantitative relation between the clearance and muzzle disturbance. In this paper, the restoring force surface (RFS method and the nonlinear identification through feedback of outputs (NIFO method are first combined for parameter identification in a simplified barrel-cradle structure. With the RFS method, clearance value can be obtained by analyzing the restoring force plot. Then the contact stiffness can be identified by using NIFO method. This identification process is verified in a single-degree-of-freedom (SDOF system with clearance. To adapt to the rigid-flexible coupled beam system with clearances which is simplified from the barrel-cradle structure, a modification for the combined method mentioned above is proposed. The core idea of the modification is reducing the continuous system to multiple-degree-of-freedom (MDOF system to reserve the nonlinear characteristics through modal transformation matrix. The advantage of this transformation is that the linear parts of the MDOF systems are decoupled, which greatly reduces the difficulty of identification. The simulation results have shown the effectiveness of current method.

  6. Resource Review Board Celebrates the Magnet and Liquid Argon Barrel Tests in Hall 180

    CERN Multimedia

    Jenni, P.

    2004-01-01

    Address by the Director-General, R. Aymar, in front of the barrel cryostat. On 25th October 2004 many RRB delegates and guests, ATLAS National Contact Physicists, and colleagues from far and from CERN working on the Liquid Argon calorimeter and the magnet system were gathering in Hall 180 to celebrate the major milestones reached during the past months in this hall: the successful cold tests of the first barrel toroid coil, of the solenoid, and of the barrel Liquid Argon calorimeter. About 250 people spent a relaxing evening after the speeches by the Director-General R. Aymar and by the spokesperson who gave the following address: 'It is a great pleasure for me to welcome you all here in Hall 180 in the name of the ATLAS Collaboration! With a few words I would like to recall why we are actually here today to share, what I hope, is a relaxed and joyful moment. To concentrate it all in one sentence I could say: To thank cordially all the main actors for the enormous work accomplished here over many years,...

  7. Dead zone analysis of ECAL barrel modules under static and dynamic load

    Science.gov (United States)

    Pierre-Emile, T.; Anduze, M.

    2018-03-01

    In the context of ILD project, impact studies of environmental loads on the Electromagnetic CALorimeter (ECAL) have been initiated. The ECAL part considered is the barrel and it consists of several independent modules which are mounted on the Hadronic CALorimeter barrel (HCAL) itself mounted on the cryostat coil and the yoke. The estimate of the gap required between each ECAL modules is fundamental to define the assembly step and avoid mechanical contacts over the barrel lifetime. In the meantime, it has to be done in consideration to the dead spaces reduction and detector hermiticity optimization. Several Finite Element Analysis (FEA) with static and dynamic loads have been performed in order to define correctly the minimum values for those gaps. Due to the implantation site of the whole project in Japan, seismic analysis were carried out in addition to the static ones. This article shows results of these analysis done with the Finite Element Method (FEM) in ANSYS. First results show the impact of HCAL design on the ECAL modules motion in static load. The second study dedicated to seismic approach on a larger model (including yoke and cryostat) gives additional results on earthquake consequences.

  8. Functions of phenylalanine residues within the beta-barrel stem of the anthrax toxin pore.

    Directory of Open Access Journals (Sweden)

    Jie Wang

    2009-07-01

    Full Text Available A key step of anthrax toxin action involves the formation of a protein-translocating pore within the endosomal membrane by the Protective Antigen (PA moiety. Formation of this transmembrane pore by PA involves interaction of the seven 2beta2-2beta3 loops of the heptameric precursor to generate a 14-strand transmembrane beta barrel.We examined the effects on pore formation, protein translocation, and cytotoxicity, of mutating two phenylalanines, F313 and F314, that lie at the tip the beta barrel, and a third one, F324, that lies part way up the barrel.Our results show that the function of these phenylalanine residues is to mediate membrane insertion and formation of stable transmembrane channels. Unlike F427, a key luminal residue in the cap of the pore, F313, F314, and F324 do not directly affect protein translocation through the pore. Our findings add to our knowledge of structure-function relationships of a key virulence factor of the anthrax bacillus.

  9. A review on the gun barrel vibrations and control for a main battle tank

    Directory of Open Access Journals (Sweden)

    Tolga Dursun

    2017-10-01

    Full Text Available Achieving high hitting accuracy for a main battle tank is challenging while the tank is on the move. This can be reached by proper design of a weapon control and gun system. In order to design an effective gun system while the tank is moving, better understanding of the dynamic behavior of the gun system is required. In this study, the dynamic behaviour of a gun system is discussed in this respect. Both experimental and numerical applications for the determination of the dynamic behaviour of a tank gun system are investigated. Methods such as the use of muzzle reference system (MRS and vibration absorbers, and active vibration control technology for the control and the reduction of the muzzle tip deflections are also reviewed. For the existing gun systems without making substantial modifications, MRS could be useful in controlling the deflections of gun barrels with estimation/prediction algorithms. The vibration levels could be cut into half by the use of optimised vibration absorbers for an existing gun. A new gun system with a longer barrel can be as accurate as the one with a short barrel with the appropriate structural modifications.

  10. Cerebral cortex modulation of pain

    Institute of Scientific and Technical Information of China (English)

    Yu-feng XIE; Fu-quan HUO; Jing-shi TANG

    2009-01-01

    Pain is a complex experience encompassing sensory-discriminative, affective-motivational and cognitiv e-emotional com-ponents mediated by different mechanisms. Contrary to the traditional view that the cerebral cortex is not involved in pain perception, an extensive cortical network associated with pain processing has been revealed using multiple methods over the past decades. This network consistently includes, at least, the anterior cingulate cortex, the agranular insular cortex, the primary (SⅠ) and secondary somatosensory (SⅡ) cortices, the ventrolateral orbital cortex and the motor cortex. These corti-cal structures constitute the medial and lateral pain systems, the nucleus submedius-ventrolateral orbital cortex-periaque-ductal gray system and motor cortex system, respectively. Multiple neurotransmitters, including opioid, glutamate, GABA and dopamine, are involved in the modulation of pain by these cortical structures. In addition, glial cells may also be in-volved in cortical modulation of pain and serve as one target for pain management research. This review discusses recent studies of pain modulation by these cerebral cortical structures in animals and human.

  11. Physiological slowing and upregulation of inhibition in cortex are correlated with behavioral deficits in protein malnourished rats.

    Directory of Open Access Journals (Sweden)

    Rahul Chaudhary

    Full Text Available Protein malnutrition during early development has been correlated with cognitive and learning disabilities in children, but the neuronal deficits caused by long-term protein deficiency are not well understood. We exposed rats from gestation up to adulthood to a protein-deficient (PD diet, to emulate chronic protein malnutrition in humans. The offspring exhibited significantly impaired performance on the 'Gap-crossing' (GC task after reaching maturity, a behavior that has been shown to depend on normal functioning of the somatosensory cortex. The physiological state of the somatosensory cortex was examined to determine neuronal correlates of the deficits in behavior. Extracellular multi-unit recording from layer 4 (L4 neurons that receive direct thalamocortical inputs and layers 2/3 (L2/3 neurons that are dominated by intracortical connections in the whisker-barrel cortex of PD rats exhibited significantly low spontaneous activity and depressed responses to whisker stimulation. L4 neurons were more severely affected than L2/3 neurons. The response onset was significantly delayed in L4 cells. The peak response latency of L4 and L2/3 neurons was delayed significantly. In L2/3 and L4 of the barrel cortex there was a substantial increase in GAD65 (112% over controls and much smaller increase in NMDAR1 (12-20%, suggesting enhanced inhibition in the PD cortex. These results show that chronic protein deficiency negatively affects both thalamo-cortical and cortico-cortical transmission during somatosensory information processing. The findings support the interpretation that sustained protein deficiency interferes with features of cortical sensory processing that are likely to underlie the cognitive impairments reported in humans who have suffered from prolonged protein deficiency.

  12. Water-Soluble Pd8L4 Self-assembled Molecular Barrel as an Aqueous Carrier for Hydrophobic Curcumin.

    Science.gov (United States)

    Bhat, Imtiyaz Ahmad; Jain, Ruchi; Siddiqui, Mujahuddin M; Saini, Deepak K; Mukherjee, Partha Sarathi

    2017-05-01

    A tetrafacial water-soluble molecular barrel (1) was synthesized by coordination driven self-assembly of a symmetrical tetrapyridyl donor (L) with a cis-blocked 90° acceptor [cis-(en)Pd(NO 3 ) 2 ] (en = ethane-1,2-diamine). The open barrel structure of (1) was confirmed by single crystal X-ray diffraction. The presence of a hydrophobic cavity with large windows makes it an ideal candidate for encapsulation and carrying hydrophobic drug like curcumin in an aqueous medium. The barrel (1) encapsulates curcumin inside its molecular cavity and protects highly photosensitive curcumin from photodegradation. The photostability of encapsulated curcumin is due to the absorption of a high proportion of the incident photons by the aromatic walls of 1 with a high absorption cross-sectional area, which helps the walls to shield the guest even against sunlight/UV radiations. As compared to free curcumin in water, we noticed a significant increase in solubility as well as cellular uptake of curcumin upon encapsulation inside the water-soluble molecular barrel (1) in aqueous medium. Fluorescence imaging confirmed that curcumin was delivered into HeLa cancer cells by the aqueous barrel (1) with the retention of its potential anticancer activity. While free curcumin is inactive toward cancer cells in aqueous medium at room temperature due to negligible solubility, the determined IC 50 value of ∼14 μM for curcumin in aqueous medium in the presence of the barrel (1) reflects the efficiency of the barrel as a potential curcumin carrier in aqueous medium without any other additives. Thus, two major challenges of increasing the bioavailability and stability of curcumin in aqueous medium even in the presence of UV light have been addressed by using a new supramolecular water-soluble barrel (1) as a drug carrier.

  13. Parameters, test criteria and fault assessment in random sampling of waste barrels from non-qualified processes

    International Nuclear Information System (INIS)

    Martens, B.R.

    1989-01-01

    In the context of random sampling tests, parameters are checked on the waste barrels and criteria are given on which these tests are based. Also, it is shown how faulty data on the properties of the waste or faulty waste barrels should be treated. To decide the extent of testing, the properties of the waste relevant to final storage are determined based on the conditioning process used. (DG) [de

  14. An outbreak of norovirus-associated acute gastroenteritis associated with contaminated barrelled water in many schools in Zhejiang, China

    Science.gov (United States)

    Sheng, Minyang; Song, Jianqiang; He, Fan; Qiu, Yinwei; Wu, Haocheng; Lu, Qinbao; Feng, Yan; Lin, Junfen; Chen, Enfu; Chai, Chengliang

    2017-01-01

    Objectives More than 900 students and teachers at many schools in Jiaxing city developed acute gastroenteritis in February 2014. An immediate epidemiological investigation was conducted to identify the pathogen, infection sources and route of transmission. Methods The probable cases and confirmed cases were defined as students or teachers with diarrhoea or vomiting present since the term began in February 2014. An active search was conducted for undiagnosed cases among students and teachers. Details such as demographic characteristics, gastrointestinal symptoms, and drinking water preference and frequency were collected via a uniform epidemiological questionnaire. A case-control study was implemented, and odds ratios (ORs) and 95% confidence intervals were calculated. Rectal swabs from several patients, food handlers and barrelled water factory workers, as well as water and food samples, were collected to test for potential bacteria and viruses. Results A total of 924 cases fit the definition of the probable case, including 8 cases of laboratory-confirmed norovirus infection at 13 schools in Jiaxing city between February 12 and February 21, 2014. The case-control study demonstrated that barrelled water was a risk factor (OR: 20.15, 95% CI: 2.59–156.76) and that bottled water and boiled barrelled water were protective factors (OR: 0.31, 95% CI: 0.13–0.70, and OR: 0.36, 95% CI: 0.16–0.77). A total of 11 rectal samples and 8 barrelled water samples were detected as norovirus-positive, and the genotypes of viral strains were the same (GII). The norovirus that contaminated the barrelled water largely came from the asymptomatic workers. Conclusions This acute gastroenteritis outbreak was caused by barrelled water contaminated by norovirus. The outbreak was controlled after stopping the supply of barrelled water. The barrelled water supply in China represents a potential source of acute gastroenteritis outbreaks due to the lack of surveillance and supervision

  15. Maps of the Auditory Cortex.

    Science.gov (United States)

    Brewer, Alyssa A; Barton, Brian

    2016-07-08

    One of the fundamental properties of the mammalian brain is that sensory regions of cortex are formed of multiple, functionally specialized cortical field maps (CFMs). Each CFM comprises two orthogonal topographical representations, reflecting two essential aspects of sensory space. In auditory cortex, auditory field maps (AFMs) are defined by the combination of tonotopic gradients, representing the spectral aspects of sound (i.e., tones), with orthogonal periodotopic gradients, representing the temporal aspects of sound (i.e., period or temporal envelope). Converging evidence from cytoarchitectural and neuroimaging measurements underlies the definition of 11 AFMs across core and belt regions of human auditory cortex, with likely homology to those of macaque. On a macrostructural level, AFMs are grouped into cloverleaf clusters, an organizational structure also seen in visual cortex. Future research can now use these AFMs to investigate specific stages of auditory processing, key for understanding behaviors such as speech perception and multimodal sensory integration.

  16. Chemosensory Learning in the Cortex

    Directory of Open Access Journals (Sweden)

    Edmund eRolls

    2011-09-01

    Full Text Available Taste is a primary reinforcer. Olfactory-taste and visual-taste association learning takes place in the primate including human orbitofrontal cortex to build representations of flavour. Rapid reversal of this learning can occur using a rule-based learning system that can be reset when an expected taste or flavour reward is not obtained, that is by negative reward prediction error, to which a population of neurons in the orbitofrontal cortex responds. The representation in the orbitofrontal cortex but not the primary taste or olfactory cortex is of the reward value of the visual / olfactory / taste / input as shown by devaluation experiments in which food is fed to satiety, and by correlations with the activations with subjective pleasantness ratings in humans. Sensory-specific satiety for taste, olfactory, visual, and oral somatosensory inputs produced by feeding a particular food to satiety are implemented it is proposed by medium-term synaptic adaptation in the orbitofrontal cortex. Cognitive factors, including word-level descriptions, modulate the representation of the reward value of food in the orbitofrontal cortex, and this effect is learned it is proposed by associative modification of top-down synapses onto neurons activated by bottom-up taste and olfactory inputs when both are active in the orbitofrontal cortex. A similar associative synaptic learning process is proposed to be part of the mechanism for the top-down attentional control to the reward value vs the sensory properties such as intensity of taste and olfactory inputs in the orbitofrontal cortex, as part of a biased activation theory of selective attention.

  17. 3D Reconstruction and Standardization of the Rat Vibrissal Cortex for Precise Registration of Single Neuron Morphology

    Science.gov (United States)

    Egger, Robert; Narayanan, Rajeevan T.; Helmstaedter, Moritz; de Kock, Christiaan P. J.; Oberlaender, Marcel

    2012-01-01

    The three-dimensional (3D) structure of neural circuits is commonly studied by reconstructing individual or small groups of neurons in separate preparations. Investigation of structural organization principles or quantification of dendritic and axonal innervation thus requires integration of many reconstructed morphologies into a common reference frame. Here we present a standardized 3D model of the rat vibrissal cortex and introduce an automated registration tool that allows for precise placement of single neuron reconstructions. We (1) developed an automated image processing pipeline to reconstruct 3D anatomical landmarks, i.e., the barrels in Layer 4, the pia and white matter surfaces and the blood vessel pattern from high-resolution images, (2) quantified these landmarks in 12 different rats, (3) generated an average 3D model of the vibrissal cortex and (4) used rigid transformations and stepwise linear scaling to register 94 neuron morphologies, reconstructed from in vivo stainings, to the standardized cortex model. We find that anatomical landmarks vary substantially across the vibrissal cortex within an individual rat. In contrast, the 3D layout of the entire vibrissal cortex remains remarkably preserved across animals. This allows for precise registration of individual neuron reconstructions with approximately 30 µm accuracy. Our approach could be used to reconstruct and standardize other anatomically defined brain areas and may ultimately lead to a precise digital reference atlas of the rat brain. PMID:23284282

  18. The design and performance of a twenty barrel hydrogen pellet injector for Alcator C-Mod

    International Nuclear Information System (INIS)

    Urbahn, J.A.

    1994-05-01

    A twenty barrel hydrogen pellet injector has been designed, built and tested both in the laboratory and on the Alcator C-Mod Tokamak at MIT. The injector functions by firing pellets of frozen hydrogen or deuterium deep into the plasma discharge for the purpose of fueling the plasma, modifying the density profile and increasing the global energy confinement time. The design goals of the injector are: (1) Operational flexibility, (2) High reliability, (3) Remote operation with minimal maintenance. These requirements have lead to a single stage, pipe gun design with twenty barrels. Pellets are formed by in- situ condensation of the fuel gas, thus avoiding moving parts at cryogenic temperatures. The injector is the first to dispense with the need for cryogenic fluids and instead uses a closed cycle refrigerator to cool the thermal system components. The twenty barrels of the injector produce pellets of four different size groups and allow for a high degree of flexibility in fueling experiments. Operation of the injector is under PLC control allowing for remote operation, interlocked safety features and automated pellet manufacturing. The injector has been extrusively tested and shown to produce pellets reliably with velocities up to 1400 m/sec. During the period from September to November of 1993, the injector was successfully used to fire pellets into over fifty plasma discharges. Experimental results include data on the pellet penetration into the plasma using an advanced pellet tracking diagnostic with improved time and spatial response. Data from the tracker indicates pellet penetrations were between 30 and 86 percent of the plasma minor radius

  19. Evaporating short-circuits in the ATLAS liquid argon barrel presampler 006

    CERN Document Server

    Belhorma, B; Lund-Jensen, B; Rydström, S; Yamouni, M

    2005-01-01

    A technique to eliminate or limit the implications of short-circuits in the ATLAS barrel presampler is described. A high voltage capacitor with a large capacity is charged at different high voltages and discharged through the short-circuit which allows either to disintegrate the dust being the origin of the short-circuit, or to burn away a thin etched copper strip which acts as a fuse on the corresponding presampler anode. This effect is possible even in the presence of a resistive HV cable (10 to 30 ohms) in series which dampens the pulse.

  20. Design, performance, and calibration of CMS hadron-barrel calorimeter wedges

    International Nuclear Information System (INIS)

    Abdullin, S.; Abramov, V.; Goncharov, P.; Khmelnikov, A.; Korablev, A.; Korneev, Y.; Krinitsyn, A.; Kryshkin, V.; Lukanin, V.; Pikalov, V.; Ryazanov, A.; Talov, V.; Turchanovich, L.; Volkov, A.; Acharya, B.; Banerjee, S.; Banerjee, S.; Chendvankar, S.; Dugad, S.; Kalmani, S.; Katta, S.; Mazumdar, K.; Mondal, N.; Nagaraj, P.; Patil, M.; Reddy, L.; Satyanarayana, B.; Sudhakar, K.; Verma, P.; Adams, M.; Burchesky, K.; Qian, W.; Akchurin, N.; Carrell, K.; Guemues, K.; Thomas, R.; Akgun, U.; Ayan, S.; Duru, F.; Merlo, J.P.; Mestvirishvili, A.; Miller, M.; Norbeck, E.; Olson, J.; Onel, Y.; Schmidt, I.; Anderson, E.W.; Hauptman, J.; Antchev, G.; Hazen, E.; Lawlor, C.; Machado, E.; Posch, C.; Rohlf, J.; Wu, S.X.; Aydin, S.; Dumanoglu, I.; Eskut, E.; Kayis-Topaksu, A.; Polatoz, A.; Onengut, G.; Ozdes-Koca, N.; Baarmand, M.; Ralich, R.; Vodopiyanov, I.; Baden, D.; Bard, R.; Eno, S.; Grassi, T.; Jarvis, C.; Kellogg, R.; Kunori, S.; Skuja, A.; Barnes, V.; Laasanen, A.; Pompos, A.; Bawa, H.; Beri, S.; Bhatnagar, V.; Kaur, M.; Kohli, J.; Kumar, A.; Singh, J.; Baiatian, G.; Sirunyan, A.; Bencze, G.; Vesztergombi, G.; Zalan, P.; Bodek, A.; Budd, H.; Chung, Y.; De Barbaro, P.; Haelen, T.; Camporesi, T.; Visser, T. de; Cankocak, K.; Cremaldi, L.; Reidy, J.; Sanders, D.A.; Cushman, P.; Sherwood, B.; Damgov, J.; Dimitrov, L.; Genchev, V.; Piperov, S.; Vankov, I.; Demianov, A.; Ershov, A.; Gribushin, A.; Kodolova, O.; Petrushanko, S.; Sarycheva, L.; Vardanyan, I.; Elias, J.; Elvira, D.; Freeman, J.; Green, D.; Los, S.; O'Dell, V.; Ronzhin, A.; Sergeyev, S.; Suzuki, I.; Vidal, R.; Whitmore, J.; Emeliantchik, I.; Massolov, V.; Shumeiko, N.; Stefanovich, R.; Fisher, W.; Tully, C.; Gavrilov, V.; Kaftanov, V.; Kisselevich, I.; Kolossov, V.; Krokhotin, A.; Kuleshov, S.; Stolin, V.; Ulyanov, A.; Gershtein, Y.; Golutvin, I.; Kalagin, V.; Kosarev, I.; Mescheryakov, G.; Smirnov, V.; Volodko, A.; Zarubin, A.; Grinev, B.; Lubinsky, V.; Senchishin, V.; Guelmez, E.; Hagopian, S.; Hagopian, V.; Johnson, K.; Heering, A.; Imboden, M.; Isiksal, E.; Karmgard, D.; Ruchti, R.; Kaya, M.; Lazic, D.; Levchuk, L.; Sorokin, P.; Litvintsev, D.; Litov, L.; Mans, J.; Ozkorucuklu, S.; Ozok, F.; Serin-Zeyrek, M.; Sever, R.; Zeyrek, M.; Paktinat, S.; Podrasky, V.; Sanzeni, C.; Winn, D.; Vlassov, E.

    2008-01-01

    Extensive measurements have been made with pions, electrons and muons on four production wedges of the compact muon solenoid (CMS) hadron barrel (HB) calorimeter in the H2 beam line at CERN with particle momenta varying from 20 to 300 GeV/c. The time structure of the events was measured with the full chain of preproduction front-end electronics running at 34 MHz. Moving-wire radioactive source data were also collected for all scintillator layers in the HB. The energy dependent time slewing effect was measured and tuned for optimal performance. (orig.)

  1. Precise Mapping of the Magnetic Field in the CMS Barrel Yoke using Cosmic Rays

    CERN Document Server

    Chatrchyan, S; Sirunyan, A M; Adam, W; Arnold, B; Bergauer, H; Bergauer, T; Dragicevic, M; Eichberger, M; Erö, J; Friedl, M; Frühwirth, R; Ghete, V M; Hammer, J; Hänsel, S; Hoch, M; Hörmann, N; Hrubec, J; Jeitler, M; Kasieczka, G; Kastner, K; Krammer, M; Liko, D; Magrans de Abril, I; Mikulec, I; Mittermayr, F; Neuherz, B; Oberegger, M; Padrta, M; Pernicka, M; Rohringer, H; Schmid, S; Schöfbeck, R; Schreiner, T; Stark, R; Steininger, H; Strauss, J; Taurok, A; Teischinger, F; Themel, T; Uhl, D; Wagner, P; Waltenberger, W; Walzel, G; Widl, E; Wulz, C E; Chekhovsky, V; Dvornikov, O; Emeliantchik, I; Litomin, A; Makarenko, V; Marfin, I; Mossolov, V; Shumeiko, N; Solin, A; Stefanovitch, R; Suarez Gonzalez, J; Tikhonov, A; Fedorov, A; Karneyeu, A; Korzhik, M; Panov, V; Zuyeuski, R; Kuchinsky, P; Beaumont, W; Benucci, L; Cardaci, M; De Wolf, E A; Delmeire, E; Druzhkin, D; Hashemi, M; Janssen, X; Maes, T; Mucibello, L; Ochesanu, S; Rougny, R; Selvaggi, M; Van Haevermaet, H; Van Mechelen, P; Van Remortel, N; Adler, V; Beauceron, S; Blyweert, S; D'Hondt, J; De Weirdt, S; Devroede, O; Heyninck, J; Kalogeropoulos, A; Maes, J; Maes, M; Mozer, M U; Tavernier, S; Van Doninck, W; Van Mulders, P; Villella, I; Bouhali, O; Chabert, E C; Charaf, O; Clerbaux, B; De Lentdecker, G; Dero, V; Elgammal, S; Gay, A P R; Hammad, G H; Marage, P E; Rugovac, S; Vander Velde, C; Vanlaer, P; Wickens, J; Grunewald, M; Klein, B; Marinov, A; Ryckbosch, D; Thyssen, F; Tytgat, M; Vanelderen, L; Verwilligen, P; Basegmez, S; Bruno, G; Caudron, J; Delaere, C; Demin, P; Favart, D; Giammanco, A; Grégoire, G; Lemaitre, V; Militaru, O; Ovyn, S; Piotrzkowski, K; Quertenmont, L; Schul, N; Beliy, N; Daubie, E; Alves, G A; Pol, M E; Souza, M H G; Carvalho, W; De Jesus Damiao, D; De Oliveira Martins, C; Fonseca De Souza, S; Mundim, L; Oguri, V; Santoro, A; Silva Do Amaral, S M; Sznajder, A; Fernandez Perez Tomei, T R; Ferreira Dias, M A; Gregores, E M; Novaes, S F; Abadjiev, K; Anguelov, T; Damgov, J; Darmenov, N; Dimitrov, L; Genchev, V; Iaydjiev, P; Piperov, S; Stoykova, S; Sultanov, G; Trayanov, R; Vankov, I; Dimitrov, A; Dyulendarova, M; Kozhuharov, V; Litov, L; Marinova, E; Mateev, M; Pavlov, B; Petkov, P; Toteva, Z; Chen, G M; Chen, H S; Guan, W; Jiang, C H; Liang, D; Liu, B; Meng, X; Tao, J; Wang, J; Wang, Z; Xue, Z; Zhang, Z; Ban, Y; Cai, J; Ge, Y; Guo, S; Hu, Z; Mao, Y; Qian, S J; Teng, H; Zhu, B; Avila, C; Baquero Ruiz, M; Carrillo Montoya, C A; Gomez, A; Gomez Moreno, B; Ocampo Rios, A A; Osorio Oliveros, A F; Reyes Romero, D; Sanabria, J C; Godinovic, N; Lelas, K; Plestina, R; Polic, D; Puljak, I; Antunovic, Z; Dzelalija, M; Brigljevic, V; Duric, S; Kadija, K; Morovic, S; Fereos, R; Galanti, M; Mousa, J; Papadakis, A; Ptochos, F; Razis, P A; Tsiakkouri, D; Zinonos, Z; Hektor, A; Kadastik, M; Kannike, K; Müntel, M; Raidal, M; Rebane, L; Anttila, E; Czellar, S; Härkönen, J; Heikkinen, A; Karimäki, V; Kinnunen, R; Klem, J; Kortelainen, M J; Lampén, T; Lassila-Perini, K; Lehti, S; Lindén, T; Luukka, P; Mäenpää, T; Nysten, J; Tuominen, E; Tuominiemi, J; Ungaro, D; Wendland, L; Banzuzi, K; Korpela, A; Tuuva, T; Nedelec, P; Sillou, D; Besancon, M; Chipaux, R; Dejardin, M; Denegri, D; Descamps, J; Fabbro, B; Faure, J L; Ferri, F; Ganjour, S; Gentit, F X; Givernaud, A; Gras, P; Hamel de Monchenault, G; Jarry, P; Lemaire, M C; Locci, E; Malcles, J; Marionneau, M; Millischer, L; Rander, J; Rosowsky, A; Rousseau, D; Titov, M; Verrecchia, P; Baffioni, S; Bianchini, L; Bluj, M; Busson, P; Charlot, C; Dobrzynski, L; Granier de Cassagnac, R; Haguenauer, M; Miné, P; Paganini, P; Sirois, Y; Thiebaux, C; Zabi, A; Agram, J L; Besson, A; Bloch, D; Bodin, D; Brom, J M; Conte, E; Drouhin, F; Fontaine, J C; Gelé, D; Goerlach, U; Gross, L; Juillot, P; Le Bihan, A C; Patois, Y; Speck, J; Van Hove, P; Baty, C; Bedjidian, M; Blaha, J; Boudoul, G; Brun, H; Chanon, N; Chierici, R; Contardo, D; Depasse, P; Dupasquier, T; El Mamouni, H; Fassi, F; Fay, J; Gascon, S; Ille, B; Kurca, T; Le Grand, T; Lethuillier, M; Lumb, N; Mirabito, L; Perries, S; Vander Donckt, M; Verdier, P; Djaoshvili, N; Roinishvili, N; Roinishvili, V; Amaglobeli, N; Adolphi, R; Anagnostou, G; Brauer, R; Braunschweig, W; Edelhoff, M; Esser, H; Feld, L; Karpinski, W; Khomich, A; Klein, K; Mohr, N; Ostaptchouk, A; Pandoulas, D; Pierschel, G; Raupach, F; Schael, S; Schultz von Dratzig, A; Schwering, G; Sprenger, D; Thomas, M; Weber, M; Wittmer, B; Wlochal, M; Actis, O; Altenhöfer, G; Bender, W; Biallass, P; Erdmann, M; Fetchenhauer, G; Frangenheim, J; Hebbeker, T; Hilgers, G; Hinzmann, A; Hoepfner, K; Hof, C; Kirsch, M; Klimkovich, T; Kreuzer, P; Lanske, D; Merschmeyer, M; Meyer, A; Philipps, B; Pieta, H; Reithler, H; Schmitz, S A; Sonnenschein, L; Sowa, M; Steggemann, J; Szczesny, H; Teyssier, D; Zeidler, C; Bontenackels, M; Davids, M; Duda, M; Flügge, G; Geenen, H; Giffels, M; Haj Ahmad, W; Hermanns, T; Heydhausen, D; Kalinin, S; Kress, T; Linn, A; Nowack, A; Perchalla, L; Poettgens, M; Pooth, O; Sauerland, P; Stahl, A; Tornier, D; Zoeller, M H; Aldaya Martin, M; Behrens, U; Borras, K; Campbell, A; Castro, E; Dammann, D; Eckerlin, G; Flossdorf, A; Flucke, G; Geiser, A; Hatton, D; Hauk, J; Jung, H; Kasemann, M; Katkov, I; Kleinwort, C; Kluge, H; Knutsson, A; Kuznetsova, E; Lange, W; Lohmann, W; Mankel, R; Marienfeld, M; Meyer, A B; Miglioranzi, S; Mnich, J; Ohlerich, M; Olzem, J; Parenti, A; Rosemann, C; Schmidt, R; Schoerner-Sadenius, T; Volyanskyy, D; Wissing, C; Zeuner, W D; Autermann, C; Bechtel, F; Draeger, J; Eckstein, D; Gebbert, U; Kaschube, K; Kaussen, G; Klanner, R; Mura, B; Naumann-Emme, S; Nowak, F; Pein, U; Sander, C; Schleper, P; Schum, T; Stadie, H; Steinbrück, G; Thomsen, J; Wolf, R; Bauer, J; Blüm, P; Buege, V; Cakir, A; Chwalek, T; De Boer, W; Dierlamm, A; Dirkes, G; Feindt, M; Felzmann, U; Frey, M; Furgeri, A; Gruschke, J; Hackstein, C; Hartmann, F; Heier, S; Heinrich, M; Held, H; Hirschbuehl, D; Hoffmann, K H; Honc, S; Jung, C; Kuhr, T; Liamsuwan, T; Martschei, D; Mueller, S; Müller, Th; Neuland, M B; Niegel, M; Oberst, O; Oehler, A; Ott, J; Peiffer, T; Piparo, D; Quast, G; Rabbertz, K; Ratnikov, F; Ratnikova, N; Renz, M; Saout, C; Sartisohn, G; Scheurer, A; Schieferdecker, P; Schilling, F P; Schott, G; Simonis, H J; Stober, F M; Sturm, P; Troendle, D; Trunov, A; Wagner, W; Wagner-Kuhr, J; Zeise, M; Zhukov, V; Ziebarth, E B; Daskalakis, G; Geralis, T; Karafasoulis, K; Kyriakis, A; Loukas, D; Markou, A; Markou, C; Mavrommatis, C; Petrakou, E; Zachariadou, A; Gouskos, L; Katsas, P; Panagiotou, A; Evangelou, I; Kokkas, P; Manthos, N; Papadopoulos, I; Patras, V; Triantis, F A; Bencze, G; Boldizsar, L; Debreczeni, G; Hajdu, C; Hernath, S; Hidas, P; Horvath, D; Krajczar, K; Laszlo, A; Patay, G; Sikler, F; Toth, N; Vesztergombi, G; Beni, N; Christian, G; Imrek, J; Molnar, J; Novak, D; Palinkas, J; Szekely, G; Szillasi, Z; Tokesi, K; Veszpremi, V; Kapusi, A; Marian, G; Raics, P; Szabo, Z; Trocsanyi, Z L; Ujvari, B; Zilizi, G; Bansal, S; Bawa, H S; Beri, S B; Bhatnagar, V; Jindal, M; Kaur, M; Kaur, R; Kohli, J M; Mehta, M Z; Nishu, N; Saini, L K; Sharma, A; Singh, A; Singh, J B; Singh, S P; Ahuja, S; Arora, S; Bhattacharya, S; Chauhan, S; Choudhary, B C; Gupta, P; Jain, S; Jha, M; Kumar, A; Ranjan, K; Shivpuri, R K; Srivastava, A K; Choudhury, R K; Dutta, D; Kailas, S; Kataria, S K; Mohanty, A K; Pant, L M; Shukla, P; Topkar, A; Aziz, T; Guchait, M; Gurtu, A; Maity, M; Majumder, D; Majumder, G; Mazumdar, K; Nayak, A; Saha, A; Sudhakar, K; Banerjee, S; Dugad, S; Mondal, N K; Arfaei, H; Bakhshiansohi, H; Fahim, A; Jafari, A; Mohammadi Najafabadi, M; Moshaii, A; Paktinat Mehdiabadi, S; Rouhani, S; Safarzadeh, B; Zeinali, M; Felcini, M; Abbrescia, M; Barbone, L; Chiumarulo, F; Clemente, A; Colaleo, A; Creanza, D; Cuscela, G; De Filippis, N; De Palma, M; De Robertis, G; Donvito, G; Fedele, F; Fiore, L; Franco, M; Iaselli, G; Lacalamita, N; Loddo, F; Lusito, L; Maggi, G; Maggi, M; Manna, N; Marangelli, B; My, S; Natali, S; Nuzzo, S; Papagni, G; Piccolomo, S; Pierro, G A; Pinto, C; Pompili, A; Pugliese, G; Rajan, R; Ranieri, A; Romano, F; Roselli, G; Selvaggi, G; Shinde, Y; Silvestris, L; Tupputi, S; Zito, G; Abbiendi, G; Bacchi, W; Benvenuti, A C; Boldini, M; Bonacorsi, D; Braibant-Giacomelli, S; Cafaro, V D; Caiazza, S S; Capiluppi, P; Castro, A; Cavallo, F R; Codispoti, G; Cuffiani, M; D'Antone, I; Dallavalle, G M; Fabbri, F; Fanfani, A; Fasanella, D; Giacomelli, P; Giordano, V; Giunta, M; Grandi, C; Guerzoni, M; Marcellini, S; Masetti, G; Montanari, A; Navarria, F L; Odorici, F; Pellegrini, G; Perrotta, A; Rossi, A M; Rovelli, T; Siroli, G; Torromeo, G; Travaglini, R; Albergo, S; Costa, S; Potenza, R; Tricomi, A; Tuve, C; Barbagli, G; Broccolo, G; Ciulli, V; Civinini, C; D'Alessandro, R; Focardi, E; Frosali, S; Gallo, E; Genta, C; Landi, G; Lenzi, P; Meschini, M; Paoletti, S; Sguazzoni, G; Tropiano, A; Benussi, L; Bertani, M; Bianco, S; Colafranceschi, S; Colonna, D; Fabbri, F; Giardoni, M; Passamonti, L; Piccolo, D; Pierluigi, D; Ponzio, B; Russo, A; Fabbricatore, P; Musenich, R; Benaglia, A; Calloni, M; Cerati, G B; D'Angelo, P; De Guio, F; Farina, F M; Ghezzi, A; Govoni, P; Malberti, M; Malvezzi, S; Martelli, A; Menasce, D; Miccio, V; Moroni, L; Negri, P; Paganoni, M; Pedrini, D; Pullia, A; Ragazzi, S; Redaelli, N; Sala, S; Salerno, R; Tabarelli de Fatis, T; Tancini, V; Taroni, S; Buontempo, S; Cavallo, N; Cimmino, A; De Gruttola, M; Fabozzi, F; Iorio, A O M; Lista, L; Lomidze, D; Noli, P; Paolucci, P; Sciacca, C; Azzi, P; Bacchetta, N; Barcellan, L; Bellan, P; Bellato, M; Benettoni, M; Biasotto, M; Bisello, D; Borsato, E; Branca, A; Carlin, R; Castellani, L; Checchia, P; Conti, E; Dal Corso, F; De Mattia, M; Dorigo, T; Dosselli, U; Fanzago, F; Gasparini, F; Gasparini, U; Giubilato, P; Gonella, F; Gresele, A; Gulmini, M; Kaminskiy, A; Lacaprara, S; Lazzizzera, I; Margoni, M; Maron, G; Mattiazzo, S; Mazzucato, M; Meneghelli, M; Meneguzzo, A T; Michelotto, M; Montecassiano, F; Nespolo, M; Passaseo, M; Pegoraro, M; Perrozzi, L; Pozzobon, N; Ronchese, P; Simonetto, F; Toniolo, N; Torassa, E; Tosi, M; Triossi, A; Vanini, S; Ventura, S; Zotto, P; Zumerle, G; Baesso, P; Berzano, U; Bricola, S; Necchi, M M; Pagano, D; Ratti, S P; Riccardi, C; Torre, P; Vicini, A; Vitulo, P; Viviani, C; Aisa, D; Aisa, S; Babucci, E; Biasini, M; Bilei, G M; Caponeri, B; Checcucci, B; Dinu, N; Fanò, L; Farnesini, L; Lariccia, P; Lucaroni, A; Mantovani, G; Nappi, A; Piluso, A; Postolache, V; Santocchia, A; Servoli, L; Tonoiu, D; Vedaee, A; Volpe, R; Azzurri, P; Bagliesi, G; Bernardini, J; Berretta, L; Boccali, T; Bocci, A; Borrello, L; Bosi, F; Calzolari, F; Castaldi, R; Dell'Orso, R; Fiori, F; Foà, L; Gennai, S; Giassi, A; Kraan, A; Ligabue, F; Lomtadze, T; Mariani, F; Martini, L; Massa, M; Messineo, A; Moggi, A; Palla, F; Palmonari, F; Petragnani, G; Petrucciani, G; Raffaelli, F; Sarkar, S; Segneri, G; Serban, A T; Spagnolo, P; Tenchini, R; Tolaini, S; Tonelli, G; Venturi, A; Verdini, P G; Baccaro, S; Barone, L; Bartoloni, A; Cavallari, F; Dafinei, I; Del Re, D; Di Marco, E; Diemoz, M; Franci, D; Longo, E; Organtini, G; Palma, A; Pandolfi, F; Paramatti, R; Pellegrino, F; Rahatlou, S; Rovelli, C; Alampi, G; Amapane, N; Arcidiacono, R; Argiro, S; Arneodo, M; Biino, C; Borgia, M A; Botta, C; Cartiglia, N; Castello, R; Cerminara, G; Costa, M; Dattola, D; Dellacasa, G; Demaria, N; Dughera, G; Dumitrache, F; Graziano, A; Mariotti, C; Marone, M; Maselli, S; Migliore, E; Mila, G; Monaco, V; Musich, M; Nervo, M; Obertino, M M; Oggero, S; Panero, R; Pastrone, N; Pelliccioni, M; Romero, A; Ruspa, M; Sacchi, R; Solano, A; Staiano, A; Trapani, P P; Trocino, D; Vilela Pereira, A; Visca, L; Zampieri, A; Ambroglini, F; Belforte, S; Cossutti, F; Della Ricca, G; Gobbo, B; Penzo, A; Chang, S; Chung, J; Kim, D H; Kim, G N; Kong, D J; Park, H; Son, D C; Bahk, S Y; Song, S; Jung, S Y; Hong, B; Kim, H; Kim, J H; Lee, K S; Moon, D H; Park, S K; Rhee, H B; Sim, K S; Kim, J; Choi, M; Hahn, G; Park, I C; Choi, S; Choi, Y; Goh, J; Jeong, H; Kim, T J; Lee, J; Lee, S; Janulis, M; Martisiute, D; Petrov, P; Sabonis, T; Castilla Valdez, H; Sánchez Hernández, A; Carrillo Moreno, S; Morelos Pineda, A; Allfrey, P; Gray, R N C; Krofcheck, D; Bernardino Rodrigues, N; Butler, P H; Signal, T; Williams, J C; Ahmad, M; Ahmed, I; Ahmed, W; Asghar, M I; Awan, M I M; Hoorani, H R; Hussain, I; Khan, W A; Khurshid, T; Muhammad, S; Qazi, S; Shahzad, H; Cwiok, M; Dabrowski, R; Dominik, W; Doroba, K; Konecki, M; Krolikowski, J; Pozniak, K; Romaniuk, Ryszard; Zabolotny, W; Zych, P; Frueboes, T; Gokieli, R; Goscilo, L; Górski, M; Kazana, M; Nawrocki, K; Szleper, M; Wrochna, G; Zalewski, P; Almeida, N; Antunes Pedro, L; Bargassa, P; David, A; Faccioli, P; Ferreira Parracho, P G; Freitas Ferreira, M; Gallinaro, M; Guerra Jordao, M; Martins, P; Mini, G; Musella, P; Pela, J; Raposo, L; Ribeiro, P Q; Sampaio, S; Seixas, J; Silva, J; Silva, P; Soares, D; Sousa, M; Varela, J; Wöhri, H K; Altsybeev, I; Belotelov, I; Bunin, P; Ershov, Y; Filozova, I; Finger, M; Finger, M., Jr.; Golunov, A; Golutvin, I; Gorbounov, N; Kalagin, V; Kamenev, A; Karjavin, V; Konoplyanikov, V; Korenkov, V; Kozlov, G; Kurenkov, A; Lanev, A; Makankin, A; Mitsyn, V V; Moisenz, P; Nikonov, E; Oleynik, D; Palichik, V; Perelygin, V; Petrosyan, A; Semenov, R; Shmatov, S; Smirnov, V; Smolin, D; Tikhonenko, E; Vasil'ev, S; Vishnevskiy, A; Volodko, A; Zarubin, A; Zhiltsov, V; Bondar, N; Chtchipounov, L; Denisov, A; Gavrikov, Y; Gavrilov, G; Golovtsov, V; Ivanov, Y; Kim, V; Kozlov, V; Levchenko, P; Obrant, G; Orishchin, E; Petrunin, A; Shcheglov, Y; Shchetkovskiy, A; Sknar, V; Smirnov, I; Sulimov, V; Tarakanov, V; Uvarov, L; Vavilov, S; Velichko, G; Volkov, S; Vorobyev, A; Andreev, Yu; Anisimov, A; Antipov, P; Dermenev, A; Gninenko, S; Golubev, N; Kirsanov, M; Krasnikov, N; Matveev, V; Pashenkov, A; Postoev, V E; Solovey, A; Toropin, A; Troitsky, S; Baud, A; Epshteyn, V; Gavrilov, V; Ilina, N; Kaftanov, V; Kolosov, V; Kossov, M; Krokhotin, A; Kuleshov, S; Oulianov, A; Safronov, G; Semenov, S; Shreyber, I; Stolin, V; Vlasov, E; Zhokin, A; Boos, E; Dubinin, M; Dudko, L; Ershov, A; Gribushin, A; Klyukhin, V; Kodolova, O; Lokhtin, I; Petrushanko, S; Sarycheva, L; Savrin, V; Snigirev, A; Vardanyan, I; Dremin, I; Kirakosyan, M; Konovalova, N; Rusakov, S V; Vinogradov, A; Akimenko, S; Artamonov, A; Azhgirey, I; Bitioukov, S; Burtovoy, V; Grishin, V; Kachanov, V; Konstantinov, D; Krychkine, V; Levine, A; Lobov, I; Lukanin, V; Mel'nik, Y; Petrov, V; Ryutin, R; Slabospitsky, S; Sobol, A; Sytine, A; Tourtchanovitch, L; Troshin, S; Tyurin, N; Uzunian, A; Volkov, A; Adzic, P; Djordjevic, M; Jovanovic, D; Krpic, D; Maletic, D; Puzovic, J; Smiljkovic, N; Aguilar-Benitez, M; Alberdi, J; Alcaraz Maestre, J; Arce, P; Barcala, J M; Battilana, C; Burgos Lazaro, C; Caballero Bejar, J; Calvo, E; Cardenas Montes, M; Cepeda, M; Cerrada, M; Chamizo Llatas, M; Clemente, F; Colino, N; Daniel, M; De La Cruz, B; Delgado Peris, A; Diez Pardos, C; Fernandez Bedoya, C; Fernández Ramos, J P; Ferrando, A; Flix, J; Fouz, M C; Garcia-Abia, P; 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Bloch, P; Bolognesi, S; Bona, M; Bos, J; Bourgeois, N; Bourrel, T; Breuker, H; Bunkowski, K; Campi, D; Camporesi, T; Cano, E; Cattai, A; Chatelain, J P; Chauvey, M; Christiansen, T; Coarasa Perez, J A; Conde Garcia, A; Covarelli, R; Curé, B; De Roeck, A; Delachenal, V; Deyrail, D; Di Vincenzo, S; Dos Santos, S; Dupont, T; Edera, L M; Elliott-Peisert, A; Eppard, M; Favre, M; Frank, N; Funk, W; Gaddi, A; Gastal, M; Gateau, M; Gerwig, H; Gigi, D; Gill, K; Giordano, D; Girod, J P; Glege, F; Gomez-Reino Garrido, R; Goudard, R; Gowdy, S; Guida, R; Guiducci, L; Gutleber, J; Hansen, M; Hartl, C; Harvey, J; Hegner, B; Hoffmann, H F; Holzner, A; Honma, A; Huhtinen, M; Innocente, V; Janot, P; Le Godec, G; Lecoq, P; Leonidopoulos, C; Loos, R; Lourenço, C; Lyonnet, A; Macpherson, A; Magini, N; Maillefaud, J D; Maire, G; Mäki, T; Malgeri, L; Mannelli, M; Masetti, L; Meijers, F; Meridiani, P; Mersi, S; Meschi, E; Meynet Cordonnier, A; Moser, R; Mulders, M; Mulon, J; Noy, M; Oh, A; Olesen, G; Onnela, A; Orimoto, T; Orsini, L; Perez, E; Perinic, G; Pernot, J F; Petagna, P; Petiot, P; Petrilli, A; Pfeiffer, A; Pierini, M; Pimiä, M; Pintus, R; Pirollet, B; Postema, H; Racz, A; Ravat, S; Rew, S B; Rodrigues Antunes, J; Rolandi, G.; Rovere, M; Ryjov, V; Sakulin, H; Samyn, D; Sauce, H; Schäfer, C; Schlatter, W D; Schröder, M; Schwick, C; Sciaba, A; Segoni, I; Sharma, A; Siegrist, N; Siegrist, P; Sinanis, N; Sobrier, T; Sphicas, P; Spiga, D; Spiropulu, M; Stöckli, F; Traczyk, P; Tropea, P; Troska, J; Tsirou, A; Veillet, L; Veres, G I; Voutilainen, M; Wertelaers, P; Zanetti, M; Bertl, W; Deiters, K; Erdmann, W; Gabathuler, K; Horisberger, R; Ingram, Q; Kaestli, H C; König, S; Kotlinski, D; Langenegger, U; Meier, F; Renker, D; Rohe, T; Sibille, J; Starodumov, A; Betev, B; Caminada, L; Chen, Z; Cittolin, S; Da Silva Di Calafiori, D R; Dambach, S; Dissertori, G; Dittmar, M; Eggel, C; Eugster, J; Faber, G; Freudenreich, K; Grab, C; Hervé, A; Hintz, W; Lecomte, P; Luckey, P D; Lustermann, W; Marchica, C; 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Neumeister, N; Sedov, A; Shipsey, I; Yoo, H D; Zheng, Y; Jindal, P; Parashar, N; Cuplov, V; Ecklund, K M; Geurts, F J M; Liu, J H; Maronde, D; Matveev, M; Padley, B P; Redjimi, R; Roberts, J; Sabbatini, L; Tumanov, A; Betchart, B; Bodek, A; Budd, H; Chung, Y S; de Barbaro, P; Demina, R; Flacher, H; Gotra, Y; Harel, A; Korjenevski, S; Miner, D C; Orbaker, D; Petrillo, G; Vishnevskiy, D; Zielinski, M; Bhatti, A; Demortier, L; Goulianos, K; Hatakeyama, K; Lungu, G; Mesropian, C; Yan, M; Atramentov, O; Bartz, E; Gershtein, Y; Halkiadakis, E; Hits, D; Lath, A; Rose, K; Schnetzer, S; Somalwar, S; Stone, R; Thomas, S; Watts, T L; Cerizza, G; Hollingsworth, M; Spanier, S; Yang, Z C; York, A; Asaadi, J; Aurisano, A; Eusebi, R; Golyash, A; Gurrola, A; Kamon, T; Nguyen, C N; Pivarski, J; Safonov, A; Sengupta, S; Toback, D; Weinberger, M; Akchurin, N; Berntzon, L; Gumus, K; Jeong, C; Kim, H; Lee, S W; Popescu, S; Roh, Y; Sill, A; Volobouev, I; Washington, E; Wigmans, R; Yazgan, E; Engh, D; Florez, C; Johns, W; Pathak, S; Sheldon, P; Andelin, D; Arenton, M W; Balazs, M; Boutle, S; Buehler, M; Conetti, S; Cox, B; Hirosky, R; Ledovskoy, A; Neu, C; Phillips II, D; Ronquest, M; Yohay, R; Gollapinni, S; Gunthoti, K; Harr, R; Karchin, P E; Mattson, M; Sakharov, A; Anderson, M; Bachtis, M; Bellinger, J N; Carlsmith, D; Crotty, I; Dasu, S; Dutta, S; Efron, J; Feyzi, F; Flood, K; Gray, L; Grogg, K S; Grothe, M; Hall-Wilton, R; Jaworski, M; Klabbers, P; Klukas, J; Lanaro, A; Lazaridis, C; Leonard, J; Loveless, R; Magrans de Abril, M; Mohapatra, A; Ott, G; Polese, G; Reeder, D; Savin, A; Smith, W H; Sourkov, A; Swanson, J; Weinberg, M; Wenman, D; Wensveen, M; White, A

    2010-01-01

    The CMS detector is designed around a large 4 T superconducting solenoid, enclosed in a 12000-tonne steel return yoke. A detailed map of the magnetic field is required for the accurate simulation and reconstruction of physics events in the CMS detector, not only in the inner tracking region inside the solenoid but also in the large and complex structure of the steel yoke, which is instrumented with muon chambers. Using a large sample of cosmic muon events collected by CMS in 2008, the field in the steel of the barrel yoke has been determined with a precision of 3 to 8% depending on the location.

  2. Upgrade of the CMS muon trigger system in the barrel region

    International Nuclear Information System (INIS)

    Rabady, Dinyar; Ero, Janos; Flouris, Giannis; Fulcher, Jonathan; Loukas, Nikitas; Paradas, Evangelos; Reis, Thomas; Sakulin, Hannes; Wulz, Claudia-Elisabeth

    2017-01-01

    To maintain the excellent performance shown during the LHC's Run-1 the Level-1 Trigger of the Compact Muon Solenoid experiment underwent a significant upgrade. One part of this upgrade is the re-organization of the muon trigger path from a subsystem-centric view in which hits in the drift tubes (DT), the cathode strip chambers (CSC), and the resistive plate chambers (RPC) were treated separately in dedicated track-finding systems to one in which complementary detector systems for a given region (barrel, overlap, and endcap) are merged at the track-finding level. This fundamental restructuring of the muon trigger system required the development of a system to receive track candidates from the track-finding layer, remove potential duplicate tracks, and forward the best candidates to the global decision layer. An overview will be given of the new track-finder system for the barrel region, the Barrel Muon Track Finder (BMTF), as well as the cancel-out and sorting layer: the upgraded Global Muon Trigger (μGMT). Both the BMTF and μGMT have been implemented in a Xilinx Virtex-7 card utilizing the microTCA architecture. While the BMTF improves on the proven and well-tested algorithms used in the Drift Tube Track Finder during Run-1, the μGMT is an almost complete re-development due to the re-organization of the underlying systems from track-finders for a specific detector to regional track finders covering a given area of the whole detector. Additionally the μGMT calculates a muon's isolation using energy information received from the calorimeter trigger. This information is added to the muon objects forwarded to the global decision layer, the so-called Global Trigger. - Highlights: • Presented upgraded Global Muon Trigger and Barrel Muon Track Finder systems. • Upgraded system moves from sub-detector centric view to geometric-view. • To improve trigger performance. • Common hardware improves maintainability and increases development speed. • Use of

  3. Precise mapping of the magnetic field in the CMS barrel yoke using cosmic rays

    Energy Technology Data Exchange (ETDEWEB)

    Chatrchyan, S. [Yerevan Physics Institute (Aremenia); et al.,

    2010-03-01

    The CMS detector is designed around a large 4 T superconducting solenoid, enclosed in a 12000-tonne steel return yoke. A detailed map of the magnetic field is required for the accurate simulation and reconstruction of physics events in the CMS detector, not only in the inner tracking region inside the solenoid but also in the large and complex structure of the steel yoke, which is instrumented with muon chambers. Using a large sample of cosmic muon events collected by CMS in 2008, the field in the steel of the barrel yoke has been determined with a precision of 3 to 8% depending on the location.

  4. The drift velocity monitoring system of the CMS barrel muon chambers

    CERN Document Server

    Altenhoefer, Georg Friedrich; Heidemann, Carsten Andreas; Reithler, Hans; Sonnenschein, Lars; Teyssier, Daniel Francois

    2017-01-01

    The drift velocity is a key parameter of drift chambers. Its value depends on several parameters: electric field, pressure, temperature, gas mixture, and contamination, for example, by ambient air. A dedicated Velocity Drift Chamber (VDC) with 1-L volume has been built at the III. Phys. Institute A, RWTH Aachen, in order to monitor the drift velocity of all CMS barrel muon Drift Tube chambers. A system of six VDCs was installed at CMS and has been running since January 2011. We present the VDC monitoring system, its principle of operation, and measurements performed.

  5. The drift velocity monitoring system of the CMS barrel muon chambers

    Science.gov (United States)

    Altenhöfer, Georg; Hebbeker, Thomas; Heidemann, Carsten; Reithler, Hans; Sonnenschein, Lars; Teyssier, Daniel

    2018-04-01

    The drift velocity is a key parameter of drift chambers. Its value depends on several parameters: electric field, pressure, temperature, gas mixture, and contamination, for example, by ambient air. A dedicated Velocity Drift Chamber (VDC) with 1-L volume has been built at the III. Phys. Institute A, RWTH Aachen, in order to monitor the drift velocity of all CMS barrel muon Drift Tube chambers. A system of six VDCs was installed at CMS and has been running since January 2011. We present the VDC monitoring system, its principle of operation, and measurements performed.

  6. Progress in the construction of the B0 model of the ATLAS Barrel Toroid magnet

    CERN Document Server

    Acerbi, E; Ambrosio, G; Baccaglioni, G; Broggi, F; Rossi, L; Sorbi, M; Volpini, G

    2000-01-01

    The ATLAS Barrel Toroid air-core magnet (BT) will be composed by 8 superconducting coils, each one 25 m long and 5 m wide. In order to validate the technologies and manufacturing processes, a smaller model (9 m long) of one BT coil, named B0, is now under construction. This paper presents a general overview of the B0 project status, with special regard to the components for which the LASA Lab. is responsible: (a) the aluminium-clad NbTi conductor; (b) the double coils winding and impregnation; (c) the components of the cryostat (vacuum chamber, thermal shield and suspension rod). (6 refs).

  7. Projects to get you off the grid rain barrels, chicken coops, and solar panels

    CERN Document Server

    Instructablescom

    2014-01-01

    Instructables is back with this compact book focused on a series of projects designed to get you thinking creatively about thinking green. Twenty Instructables illustrate just how simple it can be to make your own backyard chicken coop, or turn a wine barrel into a rainwater collector.Illustrated with dozens of full-color photographs per project accompanying easy-to-follow instructions, this Instructables collection utilizes the best that the online community has to offer, turning a far-reaching group of people into a mammoth database churning out ideas to make life better, easier, and in this

  8. The inclusion of RPC only segments in the Barrel Muon Track Finder

    CERN Document Server

    CMS Collaboration

    2018-01-01

    On November 3, 2017 during the LHC fill 6360 and from the run number 306121 RPC-only segments were enable to trigger. In this document we show the impact of the RPC-only segments in the Barrel Muon Track Finder efficiency performance. The efficiency measurement was done with Tag and Probe cut and count following the Muon POG working point recommendations (tight ID and Particle Flow isolation requirements more details can be found in https://cds.cern.ch/record/2054113). The used dataset was ZMuMu corresponding to each period.

  9. High precision, low disturbance calibration of the High Voltage system of the CMS Barrel Electromagnetic Calorimeter

    CERN Document Server

    Fasanella, Giuseppe

    2017-01-01

    The CMS Electromagnetic Calorimeter utilizes scintillating lead tungstate crystals, with avalanche photodiodes (APD) as photo-detectors in the barrel part. 1224 HV channels bias groups of 50 APD pairs, each at a voltage of about 380 V. The APD gain dependence on the voltage is 3pct/V. A stability of better than 60 mV is needed to have negligible impact on the calorimeter energy resolution. Until 2015 manual calibrations were performed yearly. A new calibration system was deployed recently, which satisfies the requirement of low disturbance and high precision. The system is discussed in detail and first operational experience is presented.

  10. High precision, low disturbance calibration of the High Voltage system of the CMS Barrel Electromagnetic Calorimeter

    CERN Document Server

    Marzocchi, Badder

    2017-01-01

    The CMS Electromagnetic Calorimeter is made of scintillating lead tungstate crystals, using avalanche photodiodes (APD) as photo-detectors in the barrel part. The high voltage system, consisting of 1224 channels, biases groups of 50 APD pairs, each at a voltage of about 380 V. The APD gain dependence on the voltage is 3pct/V. A stability of better than 60 mV is needed to have negligible impact on the calorimeter energy resolution. Until 2015 manual calibrations were performed yearly. A new calibration system was deployed recently, which satisfies the requirement of low disturbance and high precision. The system is discussed in detail and first operational experience is presented.

  11. High precision, low disturbance calibration of the High Voltage system of the CMS Barrel Electromagnetic Calorimeter

    CERN Document Server

    Fasanella, Giuseppe

    2016-01-01

    The CMS Electromagnetic Calorimeter utilizes scintillating lead tungstate crystals, with avalanche photodiodes (APD) as photo-detectors in the barrel part. 1224 HV channels bias groups of 50 APD pairs, each at a voltage of about 380 V. The APD gain dependence on the voltage is 3pct/V. A stability of better than 60 mV is needed to have negligible impact on the calorimeter energy resolution. Until 2015 manual calibrations were performed yearly. A new calibration system was deployed recently, which satisfies the requirement of low disturbance and high precision. The system is discussed in detail and first operational experience is presented.

  12. Upgrade of the CMS muon trigger system in the barrel region

    Energy Technology Data Exchange (ETDEWEB)

    Rabady, Dinyar, E-mail: dinyar.rabady@cern.ch [Institute of High Energy Physics Vienna (HEPHY), Nikolsdorfer Gasse 18, 1050 Wien (Austria); Ero, Janos [Institute of High Energy Physics Vienna (HEPHY), Nikolsdorfer Gasse 18, 1050 Wien (Austria); Flouris, Giannis [University of Ioannina, 45110 Ioannina (Greece); Fulcher, Jonathan [CERN, 1211 Geneve 23 (Switzerland); Loukas, Nikitas; Paradas, Evangelos [University of Ioannina, 45110 Ioannina (Greece); Reis, Thomas; Sakulin, Hannes; Wulz, Claudia-Elisabeth [CERN, 1211 Geneve 23 (Switzerland)

    2017-02-11

    To maintain the excellent performance shown during the LHC's Run-1 the Level-1 Trigger of the Compact Muon Solenoid experiment underwent a significant upgrade. One part of this upgrade is the re-organization of the muon trigger path from a subsystem-centric view in which hits in the drift tubes (DT), the cathode strip chambers (CSC), and the resistive plate chambers (RPC) were treated separately in dedicated track-finding systems to one in which complementary detector systems for a given region (barrel, overlap, and endcap) are merged at the track-finding level. This fundamental restructuring of the muon trigger system required the development of a system to receive track candidates from the track-finding layer, remove potential duplicate tracks, and forward the best candidates to the global decision layer. An overview will be given of the new track-finder system for the barrel region, the Barrel Muon Track Finder (BMTF), as well as the cancel-out and sorting layer: the upgraded Global Muon Trigger (μGMT). Both the BMTF and μGMT have been implemented in a Xilinx Virtex-7 card utilizing the microTCA architecture. While the BMTF improves on the proven and well-tested algorithms used in the Drift Tube Track Finder during Run-1, the μGMT is an almost complete re-development due to the re-organization of the underlying systems from track-finders for a specific detector to regional track finders covering a given area of the whole detector. Additionally the μGMT calculates a muon's isolation using energy information received from the calorimeter trigger. This information is added to the muon objects forwarded to the global decision layer, the so-called Global Trigger. - Highlights: • Presented upgraded Global Muon Trigger and Barrel Muon Track Finder systems. • Upgraded system moves from sub-detector centric view to geometric-view. • To improve trigger performance. • Common hardware improves maintainability and increases development speed. • Use of

  13. The Crystal Barrel: Meson Spectroscopy at LEAR with a 4$\\pi$ Detector

    CERN Multimedia

    2002-01-01

    % PS197 \\\\ \\\\The Crystal Barrel is a 4$\\pi$ spectrometer designed to provide complete and precise information on practically every final state produced in $\\bar{p} p $ and $\\bar{p}d $ annihilations at low energy and to collect high statistics data samples. Selective triggers can be applied when necessary. \\\\ \\\\The physics goal is to identify all light mesons in the mass range from 0.14 to 2.3~GeV/c$^{2}$, to determine their quantum numbers and decay properties and to study the annihilation dynamics. The main interest is to find the glueball and hybrid degrees of freedom predicted in the framework of Quantum Chromodynamics. \\\\ \\\\\

  14. Overview of the 63000 PWO Barrel Crystals for CMS_ECAL Production

    CERN Document Server

    Auffray, E

    2008-01-01

    In March 2007, the PWO crystal production for the barrel part of the CMS electromagnetic calorimeter has been completed. Since September 1998, 63000 crystals (61000 in Russia, 2000 in China) have been produced, received and tested in two regional centers (CERN and INFN Rome). This paper presents an overview of the procedures used from the R&D phase up to the final large scale reception and quality control. The crystals characteristics and the lessons learned from the production of this unprecedented amount of crystals in a HEP experiment are also presented.

  15. Design, performance, and calibration of CMS hadron-barrel calorimeter wedges

    Energy Technology Data Exchange (ETDEWEB)

    Abdullin, S. [Fermi National Accelerator Lab., Batavia, IL (United States)]|[Univ. of Maryland, College Park, MD (United States); Abramov, V.; Goncharov, P.; Khmelnikov, A.; Korablev, A.; Korneev, Y.; Krinitsyn, A.; Kryshkin, V.; Lukanin, V.; Pikalov, V.; Ryazanov, A.; Talov, V.; Turchanovich, L.; Volkov, A. [IHEP, Protvino (Russian Federation); Acharya, B.; Banerjee, S.; Banerjee, S.; Chendvankar, S.; Dugad, S.; Kalmani, S.; Katta, S.; Mazumdar, K.; Mondal, N.; Nagaraj, P.; Patil, M.; Reddy, L.; Satyanarayana, B.; Sudhakar, K.; Verma, P. [Tata Inst. of Fundamental Research, Mumbai (India); Adams, M.; Burchesky, K.; Qian, W. [Univ. of Illinois at Chicago, Chicago, IL (United States); Akchurin, N.; Carrell, K.; Guemues, K.; Thomas, R. [Texas Tech Univ., Dept. of Physics, Lubbock, TX (United States); Akgun, U.; Ayan, S.; Duru, F.; Merlo, J.P.; Mestvirishvili, A.; Miller, M.; Norbeck, E.; Olson, J.; Onel, Y.; Schmidt, I. [Univ. of Iowa, Iowa City, IA (United States); Anderson, E.W.; Hauptman, J. [Iowa State Univ., Ames, IA (United States); Antchev, G.; Hazen, E.; Lawlor, C.; Machado, E.; Posch, C.; Rohlf, J.; Wu, S.X. [Boston Univ., Boston, MA (United States); Aydin, S.; Dumanoglu, I.; Eskut, E.; Kayis-Topaksu, A.; Polatoz, A.; Onengut, G.; Ozdes-Koca, N. [Cukurova Univ., Adana (Turkey); Baarmand, M.; Ralich, R.; Vodopiyanov, I. [Florida Inst. of Technology, Melbourne, FL (United States); Baden, D.; Bard, R.; Eno, S.; Grassi, T.; Jarvis, C.; Kellogg, R.; Kunori, S.; Skuja, A. [Univ. of Maryland, College Park, MD (United States); Barnes, V.; Laasanen, A.; Pompos, A. [Purdue Univ., West Lafayette, IN (United States); Bawa, H.; Beri, S.; Bhatnagar, V.; Kaur, M.; Kohli, J.; Kumar, A.; Singh, J. [Panjab Univ., Chandigarh (India); Baiatian, G.; Sirunyan, A. [Yerevan Physics Inst., Yerevan (Armenia); Bencze, G.; Vesztergombi, G.; Zalan, P. [KFKI-RMKI, Research Inst. for Particle and Nuclear Physics, Budapest (Hungary)] [and others

    2008-05-15

    Extensive measurements have been made with pions, electrons and muons on four production wedges of the compact muon solenoid (CMS) hadron barrel (HB) calorimeter in the H2 beam line at CERN with particle momenta varying from 20 to 300 GeV/c. The time structure of the events was measured with the full chain of preproduction front-end electronics running at 34 MHz. Moving-wire radioactive source data were also collected for all scintillator layers in the HB. The energy dependent time slewing effect was measured and tuned for optimal performance. (orig.)

  16. Spontaneous Fluctuations of PO2 in the Rabbit Somatosensory Cortex.

    Science.gov (United States)

    Linsenmeier, Robert A; Aksenov, Daniil P; Faber, Holden M; Makar, Peter; Wyrwicz, Alice M

    2016-01-01

    In many tissues, PO2 fluctuates spontaneously with amplitudes of a few mmHg. Here we further characterized these oscillations. PO2 recordings were made from the whisker barrel cortex of six rabbits with acutely or chronically placed polarographic electrodes. Measurements were made while rabbits were awake and while anesthetized with isoflurane, during air breathing, and during 100% oxygen inspiration. In awake rabbits, 90% of the power was between 0 and 20 cycles per minute (cpm), not uniformly distributed over this range, but with a peak frequently near 10 cpm. This was much slower than heart or respiratory rhythms and is similar to the frequency content observed in other tissues. During hyperoxia, total power was higher than during air-breathing, and the dominant frequencies tended to shift toward lower values (0-10 cpm). These observations suggest that at least the lower frequency fluctuations represent efforts by the circulation to regulate local PO2. There were no consistent changes in total power during 0.5 or 1.5% isoflurane anesthesia, but the power shifted to lower frequencies. Thus, both hyperoxia and anesthesia cause characteristic, but distinct, changes in spontaneous fluctuations. These PO2 fluctuations may be caused by vasomotion, but other factors cannot be ruled out.

  17. Excitatory Neuronal Hubs Configure Multisensory Integration of Slow Waves in Association Cortex

    Directory of Open Access Journals (Sweden)

    Satoshi Kuroki

    2018-03-01

    Full Text Available Summary: Multisensory integration (MSI is a fundamental emergent property of the mammalian brain. During MSI, perceptual information encoded in patterned activity is processed in multimodal association cortex. The systems-level neuronal dynamics that coordinate MSI, however, are unknown. Here, we demonstrate intrinsic hub-like network activity in the association cortex that regulates MSI. We engineered calcium reporter mouse lines based on the fluorescence resonance energy transfer sensor yellow cameleon (YC2.60 expressed in excitatory or inhibitory neurons. In medial and parietal association cortex, we observed spontaneous slow waves that self-organized into hubs defined by long-range excitatory and local inhibitory circuits. Unlike directional source/sink-like flows in sensory areas, medial/parietal excitatory and inhibitory hubs had net-zero balanced inputs. Remarkably, multisensory stimulation triggered rapid phase-locking mainly of excitatory hub activity persisting for seconds after the stimulus offset. Therefore, association cortex tends to form balanced excitatory networks that configure slow-wave phase-locking for MSI. Video Abstract: : Kuroki et al. performed cell-type-specific, wide-field FRET-based calcium imaging to visualize cortical network activity induced by multisensory inputs. They observed phase-locking of cortical slow waves in excitatory neuronal hubs in association cortical areas that may underlie multisensory integration. Keywords: wide-field calcium imaging, multisensory integration, cortical slow waves, association cortex, phase locking, fluorescence resonance energy transfer, spontaneous activity, excitatory neuron, inhibitory neuron, mouse

  18. Upgrade of the ATLAS Muon Barrel Trigger for HL-LHC

    CERN Document Server

    Romano, Marino; The ATLAS collaboration

    2015-01-01

    The present ATLAS muon trigger in the barrel region (|eta|<1.05) is based on three layers of RPC chambers. It was designed to run for 10 years at the LHC luminosity of 10^{34} cm^{-2}s^{-1} and operated successfully and with high selectivity during the first run of the LHC. In order to ensure a stable performance of the RPCs until 2035 at the higher rates and at luminosities of 5-7x10^{34} cm^{-2}s^{-1} provided by HL-LHC, the chambers will have to be operated with reduced gas gain to respect the original design limits on currents and integrated charge. The ATLAS muon collaboration proposes an upgrade of the system by installing an inner layer of new generation RPCs during the LHC shutdown expected for the year 2023. This new layer will increase the system redundancy and therefore allow operation with high efficiency and high selectivity during the HL-LHC phase. The insertion of this new layer will also increase the geometrical acceptance in the barrel region from 75% to 95%. Moreover, the additional measu...

  19. Upgrade of the ATLAS Muon Barrel Trigger for HL-LHC.

    CERN Document Server

    Biondi, Silvia; The ATLAS collaboration

    2015-01-01

    The present ATLAS muon trigger in the barrel region (|η | < 1.05) is based on three layers of RPC chambers. It was designed to run for 10 years at the LHC luminosity of 1034cm−2s−1 and operated successfully and with high selectivity during the first run of the LHC. In order to ensure a stable performance of the RPCs until 2035 at the higher rates and at luminosities of 5−7x1034cm−2s−1 provided by HL-LHC, the chambers will have to be operated with reduced gas gain to respect the original design limits on currents and integrated charge. The ATLAS muon collaboration proposes an upgrade of the system by installing an inner layer of new generation RPCs during the LHC shutdown expected for the year 2023. This new layer will increase the system redundancy and therefore allow operation with high efficiency and high selectivity during the HL-LHC phase. The insertion of this new layer will also increase the geometrical acceptance in the barrel region from 75% to 95%. Moreover, the additional measurements ...

  20. Insertion of the first half-barrel of the ATLAS electromagnetic calorimeter into its cryostat

    CERN Multimedia

    Maximilien Brice

    2003-01-01

    The first cylinder of the ATLAS electromagnetic calorimeter barrel and the presampler have been inserted in the cryostat.The ATLAS electromagnetic calorimeter is intended to detect electrons, positrons and photons by measuring the energy they deposit on being absorbed. The cylinder of the calorimeter is in two halves, that will be sunk in a liquid-argon bath cooled to 90 kelvin (-180°C). Each half-barrel is 3.2 metres long, 53 cm thick and formed by assembling 16 modules. Each module is made up of alternate lead absorbers and electrodes pressed into 64 layers folded accordion-fashion. The presampler, set up inside the cylinder, is an integral part of the calorimeter system: It measures the energy lost by a particle before it reaches the calorimeter. To ensure an ultra-clean environment, a tent (visible here) was erected round the calorimeter and entry point to the cryostat. The detector and presampler, fitted together, could then be slid gradually into the cryostat like a drawer. To do so, the insertion team...

  1. Insertion of the first half-barrel of the ATLAS electromagnetic calorimeter into its cryostat

    CERN Multimedia

    Maximilien Brice

    2003-01-01

    The first cylinder of the ATLAS electromagnetic calorimeter barrel and the presampler have been inserted in the cryostat. The ATLAS electromagnetic calorimeter is intended to detect electrons, positrons and photons by measuring the energy they deposit on being absorbed. The cylinder of the calorimeter is in two halves, that will be sunk in a liquid-argon bath cooled to 90 kelvin (-180°C). Each half-barrel is 3.2 metres long, 53 cm thick and formed by assembling 16 modules. Each module is made up of alternate lead absorbers and electrodes pressed into 64 layers folded accordion-fashion. The presampler, set up inside the cylinder, is an integral part of the calorimeter system: It measures the energy lost by a particle before it reaches the calorimeter. To ensure an ultra-clean environment, a tent was erected round the calorimeter and entry point to the cryostat. The detector and presampler, fitted together, could then be slid gradually into the cryostat like a drawer. To do so, the insertion team had to fine-t...

  2. Installation of the Liquid Argon Calorimater Barrel in the ATLAS Experimental Cavern

    CERN Multimedia

    Vandoni, G.

    On the 27th of October, the Liquid Argon Barrel cryostat was transported from Building 180 to point 1. The next day, the Barrel was lowered into the cavern, and was placed on jacks close to its final position inside the completed lower half of the Tile calorimeter. After a day of precise adjustment, it was resting within a few millimetres of its nominal final position, waiting for the upper half of the Tile calorimeter to be installed. Tight requests had been issued by the Liquid Argon collaboration for the whole transport. It was foreseen that the cryostat should not see any acceleration larger than 0.15g along its axis, 0.08g transversally and 0.3g in the vertical direction. In addition, no acceleration higher than 0.03g (or even 0.003g for permanent oscillation) would be allowed at 20Hz, to avoid the risk of damaging the absorbers at this spontaneous vibration frequency. The difficulty would arise when coping these demands with the tortuous route, its slopes and curbs, vibration transmission from the engi...

  3. The CMS Barrel Calorimeter Response to Particle Beams from 2 to 350 GeV/c

    CERN Document Server

    Abdullin, Salavat; Acharya, Bannaje Sripathi; Adam, Nadia; Adams, Mark Raymond; Adzic, Petar; Akchurin, Nural; Akgun, Ugur; Albayrak, Elif Asli; Alemany-Fernandez, Reyes; Almeida, Nuno; Anagnostou, Georgios; Andelin, Daniel; Anderson, E Walter; Anfreville, Marc; Anicin, Ivan; Antchev, Georgy; Antunovic, Zeljko; Arcidiacono, Roberta; Arenton, Michael Wayne; Auffray, Etiennette; Argiro, Stefano; Askew, Andrew; Atramentov, Oleksiy; Ayan, S; Arcidy, M; Aydin, Sezgin; Aziz, Tariq; Baarmand, Marc M; Babich, Kanstantsin; Baccaro, Stefania; Baden, Drew; Baffioni, Stephanie; Bakirci, Mustafa Numan; Balazs, Michael; Banerjee, Sunanda; Banerjee, Sudeshna; Bard, Robert; Barge, Derek; Barnes, Virgil E; Barney, David; Barone, Luciano; Bartoloni, Alessandro; Baty, Clement; Bawa, Harinder Singh; Baiatian, G; Bandurin, Dmitry; Beauceron, Stephanie; Bell, Ken W; Bencze, Gyorgy; Benetta, Robert; Bercher, Michel; Beri, Suman Bala; Bernet, Colin; Berntzon, Lisa; Berthon, Ursula; Besançon, Marc; Betev, Botjo; Beuselinck, Raymond; Bhatnagar, Vipin; Bhatti, Anwar; Biino, Cristina; Blaha, Jan; Bloch, Philippe; Blyth, Simon; Bodek, Arie; Bornheim, Adolf; Bose, Suvadeep; Bose, Tulika; Bourotte, Jean; Brett, Angela Mary; Brown, Robert M; Britton, David; Budd, Howard; Bühler, M; Burchesky, Kyle; Busson, Philippe; Camanzi, Barbara; Camporesi, Tiziano; Cankocak, Kerem; Carrell, Kenneth Wayne; Carrera, E; Cartiglia, Nicolo; Cavallari, Francesca; Cerci, Salim; Cerutti, cM; Chang, Paoti; Chang, You-Hao; Charlot, Claude; Chen, E Augustine; Chen, Wan-Ting; Chen, Zheng-Yu; Chendvankar, Sanjay; Chipaux, Rémi; Choudhary, Brajesh C; Choudhury, Rajani Kant; Chung, Yeon Sei; Clarida, Warren; Cockerill, David J A; Combaret, Christophe; Conetti, Sergio; Cossutti, Fabio; Cox, Bradley; Cremaldi, Lucien Marcus; Cushman, Priscilla; Cussans, David; Dafinei, Ioan; Damgov, Jordan; Da Silva Di Calafiori, Diogo Raphael; Daskalakis, Georgios; Davatz, Giovanna; David, A; De Barbaro, Pawel; Debbins, Paul; Deiters, Konrad; Dejardin, Marc; Djordjevic, Milos; Deliomeroglu, Mehmet; Della Negra, Rodolphe; Della Ricca, Giuseppe; Del Re, Daniele; Demianov, A; De Min, Alberto; Denegri, Daniel; Depasse, Pierre; de Visser, Theo; Descamps, Julien; Deshpande, Pandurang Vishnu; Díaz, Jonathan; Diemoz, Marcella; Di Marco, Emanuele; Dimitrov, Lubomir; Dissertori, Günther; Dittmar, Michael; Djambazov, Lubomir; Dobrzynski, Ludwik; Drndarevic, Snezana; Duboscq, Jean Etienne; Dugad, Shashikant; Dumanoglu, Isa; Duru, Firdevs; Dutta, Dipanwita; Dzelalija, Mile; Efthymiopoulos, I; Elias, John E; Peisert, A; El-Mamouni, H; Elvira, D; Emeliantchik, Igor; Eno, Sarah Catherine; Ershov, Alexander; Erturk, Sefa; Esen, Selda; Eskut, Eda; Evangelou, Ioannis; Evans, David; Fabbro, Bernard; Faure, Jean-Louis; Fay, Jean; Fenyvesi, Andras; Ferri, Federico; Fisher, Wade Cameron; Flower, Paul S; Franci, Daniele; Franzoni, Giovanni; Freeman, Jim; Freudenreich, Klaus; Funk, Wolfgang; Ganjour, Serguei; Gargiulo, Corrado; Gascon, Susan; Gataullin, Marat; Gaultney, Vanessa; Gamsizkan, Halil; Gavrilov, Vladimir; Geerebaert, Yannick; Genchev, Vladimir; Gentit, François-Xavier; Gerbaudo, Davide; Gershtein, Yuri; Ghezzi, Alessio; Ghodgaonkar, Manohar; Gilly, Jean; Givernaud, Alain; Gleyzer, Sergei V; Gninenko, Sergei; Go, Apollo; Gobbo, Benigno; Godinovic, Nikola; Golubev, Nikolai; Golutvin, Igor; Goncharov, Petr; Gong, Datao; Govoni, Pietro; Grant, Nicholas; Gras, Philippe; Grassi, Tullio; Green, Dan; Greenhalgh, R J S; Gribushin, Andrey; Grinev, B; Guevara Riveros, Luz; Guillaud, Jean-Paul; Gurtu, Atul; Murat Guler, A; Gülmez, Erhan; Gümüs, K; Haelen, T; Hagopian, Sharon; Hagopian, Vasken; Haguenauer, Maurice; Halyo, Valerie; Hamel de Monchenault, Gautier; Hansen, Sten; Hashemi, Majid; Hauptman, John M; Hazen, Eric; Heath, Helen F; Heering, Arjan Hendrix; Heister, Arno; Heltsley, Brian; Hill, Jack; Hintz, Wieland; Hirosky, Robert; Hobson, Peter R; Honma, Alan; Hou, George Wei-Shu; Hsiung, Yee; Hunt, Adam; Husejko, Michal; Ille, Bernard; Ilyina, N; Imlay, Richard; Ingram, D; Ingram, Quentin; Isiksal, Engin; Jarry, Patrick; Jarvis, Chad; Jeong, Chiyoung; Jessop, Colin; Johnson, Kurtis F; Jones, John; Jovanovic, Dragoslav; Kaadze, Ketino; Kachanov, Vassili; Kaftanova, V; Kailas, Swaminathan; Kalagin, Vladimir; Kalinin, Alexey; Kalmani, Suresh Devendrappa; Karmgard, Daniel John; Kataria, Sushil Kumar; Kaur, Manjit; Kaya, Mithat; Kaya, Ozlem; Kayis-Topaksu, A; Kellogg, Richard G; Kennedy, Bruce W; Khmelnikov, Alexander; Kim, Heejong; Kisselevich, I; Kloukinas, Kostas; Kodolova, Olga; Kohli, Jatinder Mohan; Kokkas, Panagiotis; Kolberg, Ted; Kolossov, V; Korablev, Andrey; Korneev, Yury; Kosarev, Ivan; Kramer, Laird; Krasnikov, Nikolai; Krinitsyn, Alexander; Krokhotin, Andrey; Krpic, Dragomir; Kryshkin, V; Kubota, Yuichi; Kubrik, A; Kuleshov, Sergey; Kumar, Arun; Kumar, P; Kunori, Shuichi; Kuo, Chen-Cheng; Kurt, Pelin; Kyberd, Paul; Kyriakis, Aristotelis; Laasanen, Alvin T; Ladygin, Vladimir; Laird, Edward; Landsberg, Greg; Laszlo, Andras; Lawlor, C; Lazic, Dragoslav; Lebeau, Michel; Lecomte, Pierre; Lecoq, Paul; Ledovskoy, Alexander; Lee, Sang Joon; Leshev, Georgi; Lethuillier, Morgan; Levchuk, Leonid; Lin, Sheng-Wen; Lin, Willis; Linn, Stephan; Lintern, A L; Litvine, Vladimir; Litvintsev, Dmitri; Litov, Leander; Lobolo, L; Locci, Elizabeth; Lodge, Anthony B; Longo, Egidio; Loukas, Demetrios; Los, Serguei; Lubinsky, V; Luckey, Paul David; Lukanin, Vladimir; Lustermann, Werner; Lynch, Clare; Ma, Yousi; Machado, Emanuel; Mahlke-Krüger, H; Maity, Manas; Majumder, Gobinda; Malberti, Martina; Malclès, Julie; Maletic, Dimitrije; Mandjavidze, Irakli; Mans, Jeremy; Manthos, Nikolaos; Maravin, Yurii; Marchica, Carmelo; Marinelli, Nancy; Markou, Athanasios; Markou, Christos; Marlow, Daniel; Markowitz, Pete; Marone, Matteo; Martínez, German; Mathez, Hervé; Matveev, Viktor; Mavrommatis, Charalampos; Maurelli, Georges; Mazumdar, Kajari; Meridiani, Paolo; Merlo, Jean-Pierre; Mermerkaya, Hamit; Mescheryakov, G; Mestvirishvili, Alexi; Mikhailin, V; Milenovic, Predrag; Miller, Michael; Milleret, Gérard; Miné, Philippe; Möller, A; Mohammadi-Najafabadi, M; Mohanty, Ajit Kumar; Moissenz, P; Mondal, Naba Kumar; Moortgat, Filip; Mossolov, Vladimir; Mur, Michel; Musella, Pasquale; Musienko, Yuri; Nagaraj, P; Nardulli, Alessandro; Nash, Jordan; Nédélec, Patrick; Negri, Pietro; Newman, Harvey B; Nikitenko, Alexander; Norbeck, Edwin; Nessi-Tedaldi, Francesca; Obertino, Maria Margherita; Olson, Jonathan; Onel, Yasar; Onengüt, G; Organtini, Giovanni; Orimoto, Toyoko; Ozkan, Cigdem; Ozkurt, Halil; Ozkorucuklu, Suat; Ozok, Ferhat; Paganoni, Marco; Paganini, Pascal; Paktinat, S; Pal, Andras; Palma, Alessandro; Panev, Bozhidar; Pant, Lalit Mohan; Papadakis, Antonakis; Papadakis, Ioannis; Papadopoulos, Ioannis; Paramatti, Riccardo; Parracho, P; Pastrone, Nadia; Patil, Mandakini Ravindra; Patterson, Juliet Ritchie; Pauss, Felicitas; Penzo, Aldo; Petrakou, Eleni; Petrushanko, Sergey; Petrosian, A; Phillips II, David; Pikalov, Vladimir; Piperov, Stefan; Piroué, Pierre; Podrasky, V; Polatoz, A; Pompos, Arnold; Popescu, Sorina; Posch, C; Pozdnyakov, Andrey; Ptochos, Fotios; Puljak, Ivica; Pullia, Antonino; Punz, Thomas; Puzovic, Jovan; Qian, Weiming; Ragazzi, Stefano; Rahatlou, Shahram; Ralich, Robert; Rande, J; Razis, Panos A; Redaelli, Nicola; Reddy, L; Reidy, Jim; Renker, Dieter; Reucroft, Steve; Reymond, Jean-Marc; Ribeiro, Pedro Quinaz; Röser, Ulf; Rogalev, Evgueni; Rogan, Christopher; Roh, Youn; Rohlf, James; Romanteau, Thierry; Rondeaux, Françoise; Ronquest, Michael; Ronzhin, Anatoly; Rosowsky, André; Rovelli, Chiara; Ruchti, Randy; Rumerio, Paolo; Rusack, Roger; Rusakov, Sergey V; Ryan, Matthew John; Ryazanov, Anton; Safronov, Grigory; Sala, Leonardo; Salerno, Roberto; Sanders, David A; Santanastasio, Francesco; Sanzeni, Christopher; Sarycheva, Ludmila; Satyanarayana, B; Schinzel, Dietrich; Schmidt, Ianos; Seez, Christopher; Sekmen, Sezen; Semenov, Sergey; Senchishin, V; Sergeyev, S; Serin, Meltem; Sever, Ramazan; Sharp, Peter; Shepherd-Themistocleous, Claire; Siamitros, Christos; Sillou, Daniel; Singh, Jas Bir; Singovsky, Alexander; Sirois, Yves; Sirunyan, Albert M; Silva, J; Silva, Pedro; Skuja, Andris; Sharma, Seema; Sherwood, Brian; Shiu, Jing-Ge; Shivpuri, Ram Krishen; Shukla, Prashant; Shumeiko, Nikolai; Smirnov, Vitaly; Smith, Brian; Smith, Vincent J; Sogut, Kenan; Sonmez, Nasuf; Sorokin, Pavel; Spezziga, Mario; Sproston, Martin; Stefanovich, R; Stockli, F; Stolin, Viatcheslav; Sudhakar, Katta; Sulak, Lawrence; Suter, Henry; Suzuki, Ichiro; Swain, John; Tabarellide Fatis, T; Talov, Vladimir; Takahashi, Maiko; Tcheremoukhine, Alexandre; Teller, Olivier; Teplov, Konstantin; Theofilatos, Konstantinos; Thiebaux, Christophe; Thomas, Ray; Timciuc, Vladlen; Timlin, Claire; Titov, Maksym; Tobias, A; Tonwar, Suresh C; Topakli, Huseyin; Topkar, Anita; Triantis, Frixos A; Troshin, Sergey; Tully, Christopher; Turchanovich, L; Tyurin, Nikolay; Ueno, Koji; Ulyanov, A; Uzunian, Andrey; Vanini, A; Vankov, Ivan; Vardanyan, Irina; Varela, F; Varela, Joao; Vasil ev, A; Velasco, Mayda; Vergili, Mehmet; Verma, Piyush; Verrecchia, Patrice; Vesztergombi, Gyorgy; Veverka, Jan; Vichoudis, Paschalis; Vidal, Richard; Virdee, Tejinder; Vishnevskiy, Alexander; Vlassov, E; Vodopiyanov, Igor; Volobouev, Igor; Volkov, Alexey; Volodko, Anton; Von Gunten, Hans Peter; Wang, Lei; Wang, Minzu; Wardrope, David; Weber, Markus; Weng, Joanna; Werner, Jeremy Scott; Wetstein, Matthew; Winn, Dave; Wigmans, Richard; Williams, Jennifer C; Whitmore, Juliana; Won, Steven; Wu, Shouxiang; Yang, Yong; Yaselli, Ignacio; Yazgan, Efe; Yetkin, Taylan; Yohay, Rachel; Zabi, Alexandre; Zálán, Peter; Zamiatin, Nikolai; Zarubin, Anatoli; Zelepoukine, Serguei; Zeyrek, Mehmet; Zhang, Jia-Wen; Zhang, Lin; Zhu, Kejun; Zhu, Ren-Yuan

    2008-01-01

    The response of the CMS barrel calorimeter (electromagnetic plus hadronic) to hadrons, electrons and muons over a wide momentum range from 2 to 350 GeV/c has been measured. To our knowledge, this is the widest range of momenta in which any calorimeter system has been studied. These tests, carried out at the H2 beam-line at CERN, provide a wealth of information, especially at low energies. The analysis of the differences in calorimeter response to charged pions, kaons, protons and antiprotons and a detailed discussion of the underlying phenomena are presented. We also show techniques that apply corrections to the signals from the considerably different electromagnetic (EB) and hadronic (HB) barrel calorimeters in reconstructing the energies of hadrons. Above 5 GeV/c, these corrections improve the energy resolution of the combined system where the stochastic term equals 84.7$\\pm$1.6$\\%$ and the constant term is 7.4$\\pm$0.8$\\%$. The corrected mean response remains constant within 1.3$\\%$ rms.

  4. Qualification of barrel pixel detector modules for the Phase 1 Upgrade of the CMS vertex detector

    CERN Document Server

    Kudella, Simon

    2016-01-01

    To withstand the higher particle rates of LHC Runs 2 and 3, with expected luminosities of up to $2\\times 10^{34}\\,\\mathrm{cm^{-2}s^{-1}}$, the current CMS pixel detector at the LHC will be replaced as part of the CMS Phase I Upgrade during the extended winter shutdown in 2016/17. The new pixel detector features a new geometry with one additional detector layer in the barrel region~(BPIX) and one pair of additional disks in the forward region~(FPIX), new digital readout chips as well as a new CO$_{2}$-based cooling system for both the barrel and forward region. The BPIX detector module production is summarized, with special focus on the different stages of quality assurance. The quality tests as well as the calibrations which all produced modules undergo in a temperature and humidity controlled environment are described. Exemplarily, the KIT/Aachen production line and its subprocesses are presented together with its quality and yields.

  5. Start of operation of the barrel measuring facility II-01. Implementation into operational processes

    International Nuclear Information System (INIS)

    Buesing, B.; Escher, M.

    2013-01-01

    For the operation of the barrel measuring facility (FAME) II-01 a variety requirements to the measuring techniques were defined and tested in the frame of start-up. The used mechanical engineering and measuring technique complies with the state-of-the-art. Using the barrel measuring facility quality assured determinations of the dose rate and the nuclide-specific activity inventory were performed. For the evaluation of the gamma spectrometric measurements of FAME II-01 appropriately qualified personnel is available. The implementation of the facility in combination with the connection to the data base system PIK-AS and AVK it guaranteed that important data are available in real-time for the measuring process and the subsequent work steps. Besides this it is guaranteed that using the import/export functions relevant data are reviewed, supplemented and exchanged between the systems without transfer errors. The determined data of the dose rate and gamma spectrometric measurements allow an activity determination of the waste package with quality assurance and close to reality. Conservative assumptions in the frame of activity calculations for the later final disposal can be reduced. The automated operation of FAME allows also the reduction of radiation exposure of the personnel.

  6. A case of adenocarcinoma occurred at site of radiated double-barrel sigmoid colostomy

    Energy Technology Data Exchange (ETDEWEB)

    Ogawa, Hideaki; Kondo, Yukifumi; Takahashi, Masatoshi [Sapporo-Kosei General Hospital (Japan)] [and others

    1996-10-01

    A 60-year-old female developed an adenocarcinoma at the site of double-barrel sigmoid colostomy. She underwent a total hysterectomy and the subsequent radiation therapy due to carcinoma uteri when she was 34 years old. She had rectal stenosis one year later and underwent sigmoid colostomy. She had been healthy thereafter, however, when she was 58 years old, she noticed an elevated lesion at the site of colostomy. Biopsy revealed well-differentiated adenocarcinoma, so surgery was recommended but she refused it. Two years later, the entire stoma became a carcinoma, which directly invaded the abdominal wall on MRI images. A bow-shaped skin incision was made and en bloc resection of the tumor, colostomy and the surrounding tissues was performed. Finally, descending colostomy in the left upper quadrant of the abdomen was reconstructed. Primary adenocarcinoma occurring at a double-barrel colostomy is very rare, and such a case may never have been reported, although there were some reports of carcinoma occurring at an end colostomy. Since the site of the present carcinoma was radiated, the radiation may contribute to this carcinogenesis, suspecting its late effect. Our surgical procedure enabled simultaneously operation of combined resection and intraabdominal manipulation, and the reconstruction site of the stoma was easily selected. (author)

  7. A case of adenocarcinoma occurred at site of radiated double-barrel sigmoid colostomy

    International Nuclear Information System (INIS)

    Ogawa, Hideaki; Kondo, Yukifumi; Takahashi, Masatoshi

    1996-01-01

    A 60-year-old female developed an adenocarcinoma at the site of double-barrel sigmoid colostomy. She underwent a total hysterectomy and the subsequent radiation therapy due to carcinoma uteri when she was 34 years old. She had rectal stenosis one year later and underwent sigmoid colostomy. She had been healthy thereafter, however, when she was 58 years old, she noticed an elevated lesion at the site of colostomy. Biopsy revealed well-differentiated adenocarcinoma, so surgery was recommended but she refused it. Two years later, the entire stoma became a carcinoma, which directly invaded the abdominal wall on MRI images. A bow-shaped skin incision was made and en bloc resection of the tumor, colostomy and the surrounding tissues was performed. Finally, descending colostomy in the left upper quadrant of the abdomen was reconstructed. Primary adenocarcinoma occurring at a double-barrel colostomy is very rare, and such a case may never have been reported, although there were some reports of carcinoma occurring at an end colostomy. Since the site of the present carcinoma was radiated, the radiation may contribute to this carcinogenesis, suspecting its late effect. Our surgical procedure enabled simultaneously operation of combined resection and intraabdominal manipulation, and the reconstruction site of the stoma was easily selected. (author)

  8. Wine consumption habits and consumer preferences between wines aged in barrels or with chips.

    Science.gov (United States)

    Pérez-Magariño, Silvia; Ortega-Heras, Miriam; González-Sanjosé, María Luisa

    2011-03-30

    The use of oak wood pieces in winemaking is increasing, but the acceptance of this technique by consumers is unknown. For that reason, the main aim of this study was to measure consumers' opinion of red wines made with this new technique, their acceptance of them and their intention to purchase these wines. A preference ranking test was also carried out. A specific questionnaire was drawn up for this study and 65 frequent red wine consumers tasted four wines, two aged traditionally in barrels and two macerated with chips, and a forced choice preference test was carried out. Fifty-five per cent of respondents said that they would not buy wines made using oak chips, although most respondents would buy these wines if, after tasting them, they were as pleasant and had the same quality as the wines aged traditionally in barrels. Wines obtained with oak wood fragments were not significantly rejected either by consumers who answered the questionnaire or by consumer tasters, which could be due to the large disparity of preferences found among tasters. The results clearly indicate that producers should develop each wine taking into account the specific preferences of each consumer group. Copyright © 2011 Society of Chemical Industry.

  9. Certification and commissioning of barrel stations for the ATLAS muon spectrometer

    CERN Document Server

    Zimmermann, S

    2006-01-01

    The muon spectrometer of the ATLAS experiment, which is scheduled to commence data taking at the Large Hadron Collider, LHC at CERN in 2007, comprises more than a thousand muon stations, which have the double purpose of triggering on high-p/sub t/ muon tracks as well as providing precise trajectory reconstruction. While monitored drift tube chambers are used for track reconstruction in all of the muon spectrometer except for a region close to the beam pipe in forward direction, two different technologies are used for triggering, resistive plate chambers in the barrel region and thin gap chambers in the end-caps. Both have in common that the ATLAS geometry allows only limited accessibility after chambers are installed in the detector. A thorough testing and certification prior to installation is therefore crucial. This paper reviews the test procedure at CERN for barrel chambers of type BO and BM, i.e. of stations for which a drift chamber is coupled with one or two resistive plate chambers. The final certific...

  10. A time projection chamber for the crystal barrel experiment at ELSA

    Energy Technology Data Exchange (ETDEWEB)

    Schaab, Dimitri; Ball, Markus; Beck, Reinhard; Ketzer, Bernhard [HISKP, Bonn University (Germany); Collaboration: CBELSA/TAPS-Collaboration

    2015-07-01

    The CBELSA/TAPS experiment focuses on baryon spectroscopy by photoproduction processes off nucleons. For this purpose the experiment consists of an inner detector and an outer detector. The outer Crystal Barrel detector mainly measures photons from the decaying resonance. For charged particle identification and in order to obtain their direction, the Inner Detector consists of three layers of scintillating fibers. This inner detector will be replaced by a Time Projection Chamber (TPC). It offers improved track reconstruction capabilities, a robust pattern recognition and, if operated in a magnetic field, an excellent momentum resolution. Moreover, one obtains a particle identification of charged particles via the specific energy loss. A TPC has been developed for the FOPI experiment which also fits to the Crystal Barrel dimensions. It operates in continuous mode using Gas Electron Multipliers (GEM) as pre-amplification stage. For the TPC detector the calibration of the detector is crucial since parameters such as drift velocity or field inhomogenities have a direct impact on the detector performance. For the CBELSA TPC a calibration system is planned, which is based on the T2K calibration system. Here, the photoelectric effect is used to release electrons at well-known positions on the cathode, which drift towards the readout plane and show the integrated spatial distortions.

  11. A New Data Concentrator for the CMS Muon Barrel Track Finder

    CERN Document Server

    Triossi, Andrea

    2014-01-01

    The CMS muon trigger will undergo considerable enhancements in preparation for the LHC \\mbox{run-2}. In order to improve rate reduction and efficiency the full muon trigger chain will be completely redesigned: the plan is to move from a redundant scheme, where the three subdetectors (CSC, DT, RPC) have a separate track finder, to three geographical track finders (barrel, endcap and overlap) that combine trigger primitives of each sub-detector. In particular, the muon barrel track finder (MBTF) will host a new algorithm, that aggregating DT and RPC trigger data, will be able to improve the fake rejection and the muon momentum measurement.This report will focus on the adaptive layer of the MBTF called TwinMux. Its primary role will be to merge, arrange and fan-out the slow optical links from the chambers in faster links (10 Gbps). It will realize a full connectivity matrix between the on-detector electronics and the MBTF allowing for different processing schemes. The TwinMux will be implemented in $\\mu$TCA for...

  12. [«Man-in-the-barrel» syndrome: atypical manifestation of giant cell arteritis].

    Science.gov (United States)

    Calle-Lopez, Y; Fernandez-Ramirez, A F; Franco-Dager, E; Gomez-Lopera, J G; Vanegas-Garcia, A L

    2018-06-01

    «Man-in-the-barrel» syndrome refers to diplegia of the upper extremities in which mobility of the head and lower limbs is preserved. Brachial plexitis that presents as «man-in-the-barrel» syndrome is an unusual manifestation of giant cell arteritis. We report a case of C5-C6 plexitis as part of the clinical features of a patient with giant cell arteritis. A 70-year-old male with a two-month history of weight loss, headache, facial pain and jaw claudication, associated with a persistent elevation of acute phase reactants and bilateral brachial plexopathy, with no evidence of neck or brain injuries or occult neoplasm and with negative autoimmunity tests. Results of the biopsy study of the temporal artery were compatible with giant cell arteritis, and the positron emission tomography scan revealed extensive vascular involvement of the aorta and its branches. Although the typical clinical manifestations of giant cell arteritis are headache, jaw claudication, loss of sight, constitutional symptoms and polymyalgia rheumatica, its presence must be suspected in patients over the age of 50 who manifest alterations affecting the peripheral nerve, including brachial diplegia with no other demonstrable cause.

  13. Design and testing of a coil-unit barrel for helical coil electromagnetic launcher

    Science.gov (United States)

    Yang, Dong; Liu, Zhenxiang; Shu, Ting; Yang, Lijia; Ouyang, Jianming

    2018-01-01

    A coil-unit barrel for a helical coil electromagnetic launcher is described. It provides better features of high structural strength and flexible adjustability. It is convenient to replace the damaged coil units and easy to adjust the number of turns in the stator coils due to the modular design. In our experiments, the highest velocity measured for a 4.5-kg projectile is 47.3 m/s and the mechanical reinforcement of the launcher could bear 35 kA peak current. The relationship between the energy conversion efficiency and the inductance gradient of the launcher is also studied. In the region of low inductance gradient, the efficiency is positively correlated with the inductance gradient. However, in the region of high inductance gradient, the inter-turn arc erosion becomes a major problem of limiting the efficiency and velocity of the launcher. This modular barrel allows further studies in the inter-turn arc and the variable inductance gradient helical coil launcher.

  14. High voltage performance of a dc photoemission electron gun with centrifugal barrel-polished electrodes

    Science.gov (United States)

    Hernandez-Garcia, C.; Bullard, D.; Hannon, F.; Wang, Y.; Poelker, M.

    2017-09-01

    The design and fabrication of electrodes for direct current (dc) high voltage photoemission electron guns can significantly influence their performance, most notably in terms of maximum achievable bias voltage. Proper electrostatic design of the triple-point junction shield electrode minimizes the risk of electrical breakdown (arcing) along the insulator-cable plug interface, while the electrode shape is designed to maintain work, we describe a centrifugal barrel-polishing technique commonly used for polishing the interior surface of superconducting radio frequency cavities but implemented here for the first time to polish electrodes for dc high voltage photoguns. The technique reduced polishing time from weeks to hours while providing surface roughness comparable to that obtained with diamond-paste polishing and with unprecedented consistency between different electrode samples. We present electrode design considerations and high voltage conditioning results to 360 kV (˜11 MV/m), comparing barrel-polished electrode performance to that of diamond-paste polished electrodes. Tests were performed using a dc high voltage photogun with an inverted-geometry ceramic insulator design.

  15. Gamma ray scanner systems for nondestructive assay of heterogeneous waste barrels

    International Nuclear Information System (INIS)

    Martz, H.E.; Decman, B.J.; Roberson, G.P.; Levai, F.

    1997-01-01

    Traditional gamma safeguards measurements have usually been performed using a segmented gamma scanning (SGS) system. The accuracy of this technique relies on the assumption that the sample matrix and the activity are both uniform for a segment. Waste barrels are often highly heterogeneous, span a wide range of composition and matrix type. The primary sources of error are all directly or indirectly related to a non-uniform measurement response associated with unknown radioactive source spatial distribution and heterogeneity of the matrix. These errors can be significantly reduced by some imaging techniques that measure exact spatial locations of sources and attenuation maps. In this paper we describe a joint R ampersand D effort between the Lawrence Livermore National Laboratory (LLNL) and the Institute of Nuclear Techniques (INT) of the Technical University, Budapest, to compare results obtained by two different gamma-ray nondestructive assay (NDA) systems used for imaging waste barrels. The basic principles are the same, but the approaches are different. Key factors to judge the adequacy of a method are the detection limit and the accuracy. Test drums representing waste to be measured are used to determine basic parameters of these techniques

  16. Centralized mouse repositories.

    Science.gov (United States)

    Donahue, Leah Rae; Hrabe de Angelis, Martin; Hagn, Michael; Franklin, Craig; Lloyd, K C Kent; Magnuson, Terry; McKerlie, Colin; Nakagata, Naomi; Obata, Yuichi; Read, Stuart; Wurst, Wolfgang; Hörlein, Andreas; Davisson, Muriel T

    2012-10-01

    Because the mouse is used so widely for biomedical research and the number of mouse models being generated is increasing rapidly, centralized repositories are essential if the valuable mouse strains and models that have been developed are to be securely preserved and fully exploited. Ensuring the ongoing availability of these mouse strains preserves the investment made in creating and characterizing them and creates a global resource of enormous value. The establishment of centralized mouse repositories around the world for distributing and archiving these resources has provided critical access to and preservation of these strains. This article describes the common and specialized activities provided by major mouse repositories around the world.

  17. Sam37 is crucial for formation of the mitochondrial TOM-SAM supercomplex, thereby promoting β-barrel biogenesis.

    Science.gov (United States)

    Wenz, Lena-Sophie; Ellenrieder, Lars; Qiu, Jian; Bohnert, Maria; Zufall, Nicole; van der Laan, Martin; Pfanner, Nikolaus; Wiedemann, Nils; Becker, Thomas

    2015-09-28

    Biogenesis of mitochondrial β-barrel proteins requires two preprotein translocases, the general translocase of the outer membrane (TOM) and the sorting and assembly machinery (SAM). TOM and SAM form a supercomplex that promotes transfer of β-barrel precursors. The SAM core complex contains the channel protein Sam50, which cooperates with Sam35 in precursor recognition, and the peripheral membrane protein Sam37. The molecular function of Sam37 has been unknown. We report that Sam37 is crucial for formation of the TOM-SAM supercomplex. Sam37 interacts with the receptor domain of Tom22 on the cytosolic side of the mitochondrial outer membrane and links TOM and SAM complexes. Sam37 thus promotes efficient transfer of β-barrel precursors to the SAM complex. We conclude that Sam37 functions as a coupling factor of the translocase supercomplex of the mitochondrial outer membrane. © 2015 Wenz et al.

  18. Low-noise encoding of active touch by layer 4 in the somatosensory cortex.

    Science.gov (United States)

    Hires, Samuel Andrew; Gutnisky, Diego A; Yu, Jianing; O'Connor, Daniel H; Svoboda, Karel

    2015-08-06

    Cortical spike trains often appear noisy, with the timing and number of spikes varying across repetitions of stimuli. Spiking variability can arise from internal (behavioral state, unreliable neurons, or chaotic dynamics in neural circuits) and external (uncontrolled behavior or sensory stimuli) sources. The amount of irreducible internal noise in spike trains, an important constraint on models of cortical networks, has been difficult to estimate, since behavior and brain state must be precisely controlled or tracked. We recorded from excitatory barrel cortex neurons in layer 4 during active behavior, where mice control tactile input through learned whisker movements. Touch was the dominant sensorimotor feature, with >70% spikes occurring in millisecond timescale epochs after touch onset. The variance of touch responses was smaller than expected from Poisson processes, often reaching the theoretical minimum. Layer 4 spike trains thus reflect the millisecond-timescale structure of tactile input with little noise.

  19. Effectiveness of low impact development practices in two urbanized watersheds: retrofitting with rain barrel/cistern and porous pavement.

    Science.gov (United States)

    Ahiablame, Laurent M; Engel, Bernard A; Chaubey, Indrajeet

    2013-04-15

    The impacts of urbanization on hydrology and water quality can be minimized with the use of low impact development (LID) practices in urban areas. This study assessed the performance of rain barrel/cistern and porous pavement as retrofitting technologies in two urbanized watersheds of 70 and 40 km(2) near Indianapolis, Indiana. Six scenarios consisting of the watershed existing condition, 25% and 50% implementation of rain barrel/cistern and porous pavement, and 25% rain barrel/cistern combined with 25% porous pavement were evaluated using a proposed LID modeling framework and the Long-Term Hydrologic Impact Assessment (L-THIA)-LID model. The model was calibrated for annual runoff from 1991 to 2000, and validated from 2001 to 2010 for the two watersheds. For the calibration period, R(2) and NSE values were greater than 0.60 and 0.50 for annual runoff and streamflow. Baseflow was not calibrated in this study. During the validation period, R(2) and NSE values were greater than 0.50 for runoff and streamflow, and 0.30 for baseflow in the two watersheds. The various application levels of barrel/cistern and porous pavement resulted in 2-12% reduction in runoff and pollutant loads for the two watersheds. Baseflow loads slightly increased with increase in baseflow by more than 1%. However, reduction in runoff led to reduction in total streamflow and associated pollutant loads by 1-9% in the watersheds. The results also indicate that the application of 50% rain barrel/cistern, 50% porous pavement and 25% rain barrel/cistern combined with 25% porous pavement are good retrofitting options in these watersheds. The L-THIA-LID model can be used to inform management and decision-making for implementation of LID practices at the watershed scale. Copyright © 2013. Published by Elsevier Ltd.

  20. MRI volumetry of prefrontal cortex

    Science.gov (United States)

    Sheline, Yvette I.; Black, Kevin J.; Lin, Daniel Y.; Pimmel, Joseph; Wang, Po; Haller, John W.; Csernansky, John G.; Gado, Mokhtar; Walkup, Ronald K.; Brunsden, Barry S.; Vannier, Michael W.

    1995-05-01

    Prefrontal cortex volumetry by brain magnetic resonance (MR) is required to estimate changes postulated to occur in certain psychiatric and neurologic disorders. A semiautomated method with quantitative characterization of its performance is sought to reliably distinguish small prefrontal cortex volume changes within individuals and between groups. Stereological methods were tested by a blinded comparison of measurements applied to 3D MR scans obtained using an MPRAGE protocol. Fixed grid stereologic methods were used to estimate prefrontal cortex volumes on a graphic workstation, after the images are scaled from 16 to 8 bits using a histogram method. In addition images were resliced into coronal sections perpendicular to the bicommissural plane. Prefrontal cortex volumes were defined as all sections of the frontal lobe anterior to the anterior commissure. Ventricular volumes were excluded. Stereological measurement yielded high repeatability and precision, and was time efficient for the raters. The coefficient of error was volumetry by stereology can yield accurate and repeatable measurements. Small frontal lobe volume reductions in patients with brain disorders such as depression and schizophrenia can be efficiently assessed using this method.

  1. Development and test of a prototype for the PANDA barrel DIRC detector at FAIR

    Energy Technology Data Exchange (ETDEWEB)

    Kalicy, Grzegorz

    2014-07-01

    The PANDA experiment at FAIR will perform world class physics studies using high-intensity cooled antiproton beams with momenta between 1.5 and 15 GeV/c. A rich physics program requires very good particle identification (PID). Charged hadron PID for the barrel section of the target spectrometer has to cover the angular range of 22-140 and separate pions from kaons for momenta up to 3.5 GeV/c with a separation power of at least 3 standard deviations. The system that will provide it has to be thin and operate in a strong magnetic field. A ring imaging Cherenkov detector using the DIRC principle meets those requirements. The design of the PANDA Barrel DIRC is based on the successful BABAR DIRC counter with several important changes to improve the performance and optimize the costs. The design options are being studied in detailed Monte Carlo simulation, and implemented in increasingly complex system prototypes and tested in particle beams. Before building the full system prototypes the radiator bars and lenses are measured on the test benches. The performance of the DIRC prototype was quantified in terms of the single photon Cherenkov angle resolution and the photon yield. Results for two full system prototypes will be presented. The prototype in 2011 aimed at investigating the full size expansion volume. It was found that the resolution for this configuration is at the level of in good agreement with ray tracing simulation results. A more complex prototype, tested in 2012, provided the first experience with a compact fused silica prism expansion volume, a wide radiator plate, and several advanced lens options for the focusing system. The performance of the baseline configuration of the prototype with a standard lens and an air gap met the requirements for the PANDA PID for most of the polar angle range but failed at polar angles around 90 due to photon loss at the air gap. Measurements with a prototype high-refractive index compound lens without an air gap at a polar

  2. Development and test of a prototype for the PANDA barrel DIRC detector at FAIR

    International Nuclear Information System (INIS)

    Kalicy, Grzegorz

    2014-01-01

    The PANDA experiment at FAIR will perform world class physics studies using high-intensity cooled antiproton beams with momenta between 1.5 and 15 GeV/c. A rich physics program requires very good particle identification (PID). Charged hadron PID for the barrel section of the target spectrometer has to cover the angular range of 22-140 and separate pions from kaons for momenta up to 3.5 GeV/c with a separation power of at least 3 standard deviations. The system that will provide it has to be thin and operate in a strong magnetic field. A ring imaging Cherenkov detector using the DIRC principle meets those requirements. The design of the PANDA Barrel DIRC is based on the successful BABAR DIRC counter with several important changes to improve the performance and optimize the costs. The design options are being studied in detailed Monte Carlo simulation, and implemented in increasingly complex system prototypes and tested in particle beams. Before building the full system prototypes the radiator bars and lenses are measured on the test benches. The performance of the DIRC prototype was quantified in terms of the single photon Cherenkov angle resolution and the photon yield. Results for two full system prototypes will be presented. The prototype in 2011 aimed at investigating the full size expansion volume. It was found that the resolution for this configuration is at the level of in good agreement with ray tracing simulation results. A more complex prototype, tested in 2012, provided the first experience with a compact fused silica prism expansion volume, a wide radiator plate, and several advanced lens options for the focusing system. The performance of the baseline configuration of the prototype with a standard lens and an air gap met the requirements for the PANDA PID for most of the polar angle range but failed at polar angles around 90 due to photon loss at the air gap. Measurements with a prototype high-refractive index compound lens without an air gap at a polar

  3. The Functions of the Orbitofrontal Cortex

    Science.gov (United States)

    Rolls, Edmund T.

    2004-01-01

    The orbitofrontal cortex contains the secondary taste cortex, in which the reward value of taste is represented. It also contains the secondary and tertiary olfactory cortical areas, in which information about the identity and also about the reward value of odours is represented. The orbitofrontal cortex also receives information about the sight…

  4. Cholinergic axon length reduced by 300 meters in the brain of an Alzheimer mouse model

    DEFF Research Database (Denmark)

    Nikolajsen, Gitte; Jensen, Morten Skovgaard; West, Mark J.

    2011-01-01

    Modern stereological techniques have been used to show that the total length of the cholinergic fibers in the cerebral cortex of the APPswe/PS1deltaE9 mouse is reduced by almost 300 meters at 18 months of age and has a nonlinear relationship to the amount of transgenetically-induced amyloidosis. ....... These data provide rigorous quantitative morphological evidence that Alzheimer's-like amyloidosis affects the axons of the cholinergic enervation of the cerebral cortex....

  5. Hadron Energy Reconstruction for ATLAS Barrel Combined Calorimeter Using Non-Parametrical Method

    CERN Document Server

    Kulchitskii, Yu A

    2000-01-01

    Hadron energy reconstruction for the ATLAS barrel prototype combined calorimeter in the framework of the non-parametrical method is discussed. The non-parametrical method utilizes only the known e/h ratios and the electron calibration constants and does not require the determination of any parameters by a minimization technique. Thus, this technique lends itself to fast energy reconstruction in a first level trigger. The reconstructed mean values of the hadron energies are within \\pm1% of the true values and the fractional energy resolution is [(58\\pm 3)%{\\sqrt{GeV}}/\\sqrt{E}+(2.5\\pm0.3)%]\\bigoplus(1.7\\pm0.2) GeV/E. The value of the e/h ratio obtained for the electromagnetic compartment of the combined calorimeter is 1.74\\pm0.04. Results of a study of the longitudinal hadronic shower development are also presented.

  6. Design optimisation of the ATLAS Barrel Toroid structure - the warm structure

    International Nuclear Information System (INIS)

    Daeel, A.; Desvard, J-P.; Pabot, Y.; Sun, Z.; Hille, H. van; Vedrine, P.

    2001-01-01

    The magnetic bending of muon tracks for the ATLAS Muon Spectrometer is provided by the large air-core toroid magnets. The Barrel Toroid structure, named the warm structure, is an open structure inside which the muon chambers are installed. The physics performance of the muon spectrometer imposes stringent requirements on the design of the warm structure. It should support the muon chambers with required precision and stability, the deformation of the structure must be minimised. At the same time, the quantities of the materials used in the structure must also be minimised. Through extensive structural analyses, the design optimisation has been achieved to fit with the physics requirements. This paper gives an overview on the design considerations of the warm structure

  7. Non-compensation of the ATLAS barrel tile hadron module-0 calorimeter

    International Nuclear Information System (INIS)

    Kul'chitskij, Yu.A.; Vinogradov, V.B.

    1999-01-01

    The detailed experimental information about the electron and pion responses, the electron energy resolution and the elh ratio as a function of incident energy E, impact point Z and incidence angle Θ of the Module-0 of the ATLAS iron-scintillator barrel hadron calorimeter with the longitudinal tile configuration is presented. The results are based on the electron and pion beams data for E = 10, 20, 60, 80, 100 and 180 GeV at η = - 0.25 and -0.55, which have been obtained during the test beam period in 1996. The results are compared with the existing experimental data of TILECAL 1m prototype modules, various iron-scintillator calorimeters and with some Monte Carlo calculations

  8. STAR barrel electromagnetic calorimeter absolute calibration using 'minimum ionizing particles' from collisions at RHIC

    International Nuclear Information System (INIS)

    Cormier, T.M.; Pavlinov, A.I.; Rykov, M.V.; Rykov, V.L.; Shestermanov, K.E.

    2002-01-01

    The procedure for the STAR Barrel Electromagnetic Calorimeter (BEMC) absolute calibrations, using penetrating charged particle hits (MIP-hits) from physics events at RHIC, is presented. Its systematic and statistical errors are evaluated. It is shown that, using this technique, the equalization and transfer of the absolute scale from the test beam can be done to a percent level accuracy in a reasonable amount of time for the entire STAR BEMC. MIP-hits would also be an effective tool for continuously monitoring the variations of the BEMC tower's gains, virtually without interference to STAR's main physics program. The method does not rely on simulations for anything other than geometric and some other small corrections, and also for estimations of the systematic errors. It directly transfers measured test beam responses to operations at RHIC

  9. Optimizing Centrifugal Barrel Polishing For Mirror Finish SRF Cavity And RF Tests At Jefferson Lab

    International Nuclear Information System (INIS)

    Palczewski, Ari; Geng, Rongli; Tian, Hui

    2012-01-01

    We performed Centrifugal Barrel Polishing (CBP) on a 1.3 GHz fine grain TESLA single cell cavity and 1.5 GHz fine grain CEBAF high gradient superconducting radio frequency (SRF) single cell cavity following a modified recipe originally developed at Fermi National Accelerator Lab (FNAL). We were able to obtain a mirror like surface similar to that obtained at FNAL, while reducing the number of CBP steps and total processing time. This paper will discuss the change in surface and subsequent cavity performance post CBP, after a 800 C bake (no pre-bake chemistry) and minimal controlled electro-polishing (10 micron). In addition to Q vs. E ACC thermometry mapping with preheating characteristics and optical inspection of the cavity after CBP will also be shown.

  10. Design, Performance, and Calibration of CMS Hadron-Barrel Calorimeter Wedges

    CERN Document Server

    Baiatian, G; Emeliantchik, Igor; Massolov, V; Shumeiko, Nikolai; Stefanovich, R; Damgov, Jordan; Dimitrov, Lubomir; Genchev, Vladimir; Piperov, Stefan; Vankov, Ivan; Litov, Leander; Bencze, Gyorgy; Vesztergombi, Gyorgy; Zálán, Peter; Bawa, Harinder Singh; Beri, Suman Bala; Bhatnagar, Vipin; Kaur, Manjit; Kohli, Jatinder Mohan; Kumar, Arun; Singh, Jas Bir; Acharya, Bannaje Sripathi; Banerjee, Sunanda; Banerjee, Sudeshna; Chendvankar, Sanjay; Dugad, Shashikant; Kalmani, Suresh Devendrappa; Katta, S; Mazumdar, Kajari; Mondal, Naba Kumar; Nagaraj, P; Patil, Mandakini Ravindra; Reddy, L; Satyanarayana, B; Sudhakar, Katta; Verma, Piyush; Paktinat, S; Golutvin, Igor; Kalagin, Vladimir; Kosarev, Ivan; Mescheryakov, G; Sergeyev, S; Smirnov, Vitaly; Volodko, Anton; Zarubin, Anatoli; Gavrilov, Vladimir; Gershtein, Yuri; Kaftanov, Vitali; Kisselevich, I; Kolossov, V; Krokhotin, Andrey; Kuleshov, Sergey; Litvintsev, Dmitri; Stolin, Viatcheslav; Ulyanov, A; Demianov, A; Gribushin, Andrey; Kodolova, Olga; Petrushanko, Sergey; Sarycheva, Ludmila; Vardanyan, Irina; Yershov, A; Abramov, Victor; Goncharov, Petr; Khmelnikov, Alexander; Korablev, Andrey; Korneev, Yury; Krinitsyn, Alexander; Kryshkin, V; Lukanin, Vladimir; Pikalov, Vladimir; Ryazanov, Anton; Talov, Vladimir; Turchanovich, L; Volkov, Alexey; Camporesi, Tiziano; De Visser, Theo; Vlassov, E; Aydin, Sezgin; Dumanoglu, Isa; Eskut, Eda; Kayis-Topaksu, A; Kuzucu-Polatoz, A; Onengüt, G; Ozdes-Koca, N; Cankocak, Kerem; Ozok, Ferhat; Serin-Zeyrek, M; Sever, Ramazan; Zeyrek, Mehmet; Gülmez, Erhan; Isiksal, Engin; Kaya, Mithat; Ozkorucuklu, Suat; Levchuk, Leonid; Sorokin, Pavel; Grinev, B; Lubinsky, V; Senchishin, V; Anderson, E Walter; Hauptman, John M; Elias, John E; Elvira, D; Freeman, Jim; Green, Dan; Lazic, Dragoslav; Los, Serguei; O'Dell, Vivian; Ronzhin, Anatoly; Suzuki, Ichiro; Vidal, Richard; Whitmore, Juliana; Antchev, Georgy; Hazen, Eric; Lawlor, C; Machado, Emanuel; Posch, C; Rohlf, James; Wu, Shouxiang; Adams, Mark Raymond; Burchesky, Kyle; Qiang, W; Abdullin, Salavat; Baden, Drew; Bard, Robert; Eno, Sarah Catherine; Grassi, Tullio; Jarvis, Chad; Kellogg, Richard G; Kunori, Shuichi; Skuja, Andris; Podrasky, V; Sanzeni, Christopher; Winn, Dave; Akgun, Ugur; Ayan, S; Duru, Firdevs; Merlo, Jean-Pierre; Mestvirishvili, Alexi; Miller, Michael; Norbeck, Edwin; Olson, Jonathan; Onel, Yasar; Schmidt, Ianos; Akchurin, Nural; Carrell, Kenneth Wayne; Gumu, K; Thomas, Ray; Baarmand, Marc M; Ralich, Robert; Vodopiyanov, Igor; Cushman, Priscilla; Heering, Arjan Hendrix; Sherwood, Brian; Cremaldi, Lucien Marcus; Reidy, Jim; Sanders, David A; Karmgard, Daniel John; Ruchti, Randy; Fisher, Wade Cameron; Mans, Jeremy; Tully, Christopher; De Barbaro, Pawel; Bodek, Arie; Budd, Howard; Chung, Yeon Sei; Haelen, T; Imboden, Matthias; Hagopian, Sharon; Hagopian, Vasken; Johnson, Kurtis F; Barnes, Virgil E; Laasanen, Alvin T; Pompos, Arnold

    2007-01-01

    Extensive measurements have been made with pions, electrons and muons on four production wedges of the Compact Muon Solenoid (CMS) hadron barrel (HB) calorimeter in the H2 beam line at CERN with particle momenta varying from 20 to 300 GeV/c. Data were taken both with and without a prototype electromagnetic lead tungstate crystal calorimeter (EB) in front of the hadron calorimeter. The time structure of the events was measured with the full chain of preproduction front-end electronics running at 34 MHz. Moving-wire radioactive source data were also collected for all scintillator layers in the HB. These measurements set the absolute calibration of the HB prior to first pp collisions to approximately 4%.

  11. High performance interactive graphics for shower reconstruction in HPC, the DELPHI barrel electromagnetic calorimeter

    International Nuclear Information System (INIS)

    Stanescu, C.

    1990-01-01

    Complex software for shower reconstruction in DELPHI barrel electromagnetic calorimeter which deals, for each event, with great amounts of information, due to the high spatial resolution of this detector, needs powerful verification tools. An interactive graphics program, running on high performance graphics display system Whizzard 7555 from Megatek, was developed to display the logical steps in showers and their axes reconstruction. The program allows both operations on the image in real-time (rotation, translation and zoom) and the use of non-geometrical criteria to modify it (as the use of energy) thresholds for the representation of the elements that compound the showers (or of the associated lego plots). For this purpose graphics objects associated to user parameters were defined. Instancing and modelling features of the native graphics library were extensively used

  12. Application of linear accelerator technology to the detection of trace amounts of transuranics in waste barrels

    International Nuclear Information System (INIS)

    Cates, M.R.; Noel, B.W.; Caldwell, J.T.; Kunz, W.E.; Close, D.A.; Franks, L.A.; Pigg, J.L.

    1980-01-01

    Electron linear accelerators (linacs), as sources of photons and neutrons, can produce a significant number of fissions in transuranic isotopes contained in large barrels of waste material. Both photons and thermal neutrons have been used to detect about 1 mg of plutonium in 105-kg matrices. A sequential interrogation with neutrons and photons, easily possible with linacs, can show both fertile and fissile constituents among the heavy-mass isotopes. The advantages of linacs in solving existing assay problems include: (1) high available beam current; (2) variable beam current, beam energy, pulse width, and pulse repetition frequency; and (3) beam-scanning ability. They also are compatible with passive assay instruments. Their versatility makes it likely that they will remain useful as assay technology advances

  13. Geometrical principles of homomeric β-barrels and β-helices: Application to modeling amyloid protofilaments.

    Science.gov (United States)

    Hayward, Steven; Milner-White, E James

    2017-10-01

    Examples of homomeric β-helices and β-barrels have recently emerged. Here we generalize the theory for the shear number in β-barrels to encompass β-helices and homomeric structures. We introduce the concept of the "β-strip," the set of parallel or antiparallel neighboring strands, from which the whole helix can be generated giving it n-fold rotational symmetry. In this context, the shear number is interpreted as the sum around the helix of the fixed register shift between neighboring identical β-strips. Using this approach, we have derived relationships between helical width, pitch, angle between strand direction and helical axis, mass per length, register shift, and number of strands. The validity and unifying power of the method is demonstrated with known structures including α-hemolysin, T4 phage spike, cylindrin, and the HET-s(218-289) prion. From reported dimensions measured by X-ray fiber diffraction on amyloid fibrils, the relationships can be used to predict the register shift and the number of strands within amyloid protofilaments. This was used to construct models of transthyretin and Alzheimer β(40) amyloid protofilaments that comprise a single strip of in-register β-strands folded into a "β-strip helix." Results suggest both stabilization of an individual β-strip helix and growth by addition of further β-strip helices can involve the same pair of sequence segments associating with β-sheet hydrogen bonding at the same register shift. This process would be aided by a repeat sequence. Hence, understanding how the register shift (as the distance between repeat sequences) relates to helical dimensions will be useful for nanotube design. © 2017 Wiley Periodicals, Inc.

  14. Insights into the fold organization of TIM barrel from interaction energy based structure networks.

    Science.gov (United States)

    Vijayabaskar, M S; Vishveshwara, Saraswathi

    2012-01-01

    There are many well-known examples of proteins with low sequence similarity, adopting the same structural fold. This aspect of sequence-structure relationship has been extensively studied both experimentally and theoretically, however with limited success. Most of the studies consider remote homology or "sequence conservation" as the basis for their understanding. Recently "interaction energy" based network formalism (Protein Energy Networks (PENs)) was developed to understand the determinants of protein structures. In this paper we have used these PENs to investigate the common non-covalent interactions and their collective features which stabilize the TIM barrel fold. We have also developed a method of aligning PENs in order to understand the spatial conservation of interactions in the fold. We have identified key common interactions responsible for the conservation of the TIM fold, despite high sequence dissimilarity. For instance, the central beta barrel of the TIM fold is stabilized by long-range high energy electrostatic interactions and low-energy contiguous vdW interactions in certain families. The other interfaces like the helix-sheet or the helix-helix seem to be devoid of any high energy conserved interactions. Conserved interactions in the loop regions around the catalytic site of the TIM fold have also been identified, pointing out their significance in both structural and functional evolution. Based on these investigations, we have developed a novel network based phylogenetic analysis for remote homologues, which can perform better than sequence based phylogeny. Such an analysis is more meaningful from both structural and functional evolutionary perspective. We believe that the information obtained through the "interaction conservation" viewpoint and the subsequently developed method of structure network alignment, can shed new light in the fields of fold organization and de novo computational protein design.

  15. Usage of FT-ICR-MS Metabolomics for characterizing the chemical signatures of barrel-aged whisky

    Science.gov (United States)

    Roullier-Gall, Chloé; Signoret, Julie; Hemmler, Daniel; Witting, Michael A.; Kanawati, Basem; Schäfer, Bernhard; Gougeon, Régis D.; Schmitt-Kopplin, Philippe

    2018-02-01

    Whisky can be described as a complex matrix integrating the chemical history from the fermented cereals, the wooden barrels, the specific distillery processes, ageing and environmental factors. In this study, using Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS), we analysed 150 whisky samples from 49 different distilleries, 7 countries, and ranging from 1 day new make spirit to 43 years of maturation with different types of barrel. Chemometrics revealed the unexpected impact of the wood history on the distillatés composition during barrel ageing, regardless of the whisky origin. Flavonols, oligolignols and fatty acids are examples of important chemical signatures for Bourbon casks, whereas a high number of polyphenol glycosides, including for instance quercetin-glucuronide or myricetin-glucoside as potential candidates, and carbohydrates would discriminate Sherry casks. However, the comparison of barrel aged rums and whiskies revealed specific signatures, highlighting the importance of the initial composition of the distillate and the distillery processes.

  16. A cosmic ray muon recorded by the ATLAS barrel tile calorimeter at 18:30, on 21 June 2005.

    CERN Multimedia

    2005-01-01

    The ATLAS barrel tile calorimeter has recorded its first events underground using a cosmic ray trigger, as part of the detector commissioning programme. The calorimeter has three layers and a pointing geometry. The light trapezoids represent the energy deposited in the tiles of the calorimeter depicted as a thick disk.

  17. Lock, Stock and Two Different Barrels: Comparing the Genetic Composition of Morphotypes of the Indo-Pacific Sponge

    NARCIS (Netherlands)

    Swierts, T.; Peijnenburg, K.T.C.A.; de Leeuw, C.; Cleary, D.F.R.; Hörnlein, C.; Setiawan, E.; Wörheide, G.; Erpenbeck, D.; de Voogd, N.J.

    2013-01-01

    The giant barrel sponge Xestospongia testudinaria is an ecologically important species that is widely distributed across the Indo-Pacific. Little is known, however, about the precise biogeographic distribution and the amount of morphological and genetic variation in this species. Here we provide the

  18. Lock, stock and two different barrels: comparing the genetic composition of morphotypes of the Indo-Pacific sponge Xestospongia testudinaria

    NARCIS (Netherlands)

    Swierts, T.; Peijnenburg, K.T.C.A.; Leeuw, de C.; Cleary, D.F.R.; Hörnlein, C.; Setiawan, E.; Wörheide, G.; Erpenbeck, D.; Voogd, de N.J.

    2013-01-01

    The giant barrel sponge Xestospongia testudinaria is an ecologically important species that is widely distributed across the Indo-Pacific. Little is known, however, about the precise biogeographic distribution and the amount of morphological and genetic variation in this species. Here we provide the

  19. Lock, Stock and two different barrels: comparing the genetic composition of morphotypes of the Indo-Pacific sponge Xestospongia testudinaria

    NARCIS (Netherlands)

    Swierts, T.; Peijnenburg, K.; de Leeuw, C.; Cleary, D.F.R.; Hörnlein, C.; Setiawan, E.; Wörheide, G.; Erpenbeck, D.; de Voogd, N.J.

    2013-01-01

    The giant barrel sponge Xestospongia testudinaria is an ecologically important species that is widely distributed across the Indo-Pacific. Little is known, however, about the precise biogeographic distribution and the amount of morphological and genetic variation in this species. Here we provide the

  20. Coevolving residues of (beta/alpha)(8)-barrel proteins play roles in stabilizing active site architecture and coordinating protein dynamics.

    Science.gov (United States)

    Shen, Hongbo; Xu, Feng; Hu, Hairong; Wang, Feifei; Wu, Qi; Huang, Qiang; Wang, Honghai

    2008-12-01

    Indole-3-glycerol phosphate synthase (IGPS) is a representative of (beta/alpha)(8)-barrel proteins-the most common enzyme fold in nature. To better understand how the constituent amino-acids work together to define the structure and to facilitate the function, we investigated the evolutionary and dynamical coupling of IGPS residues by combining statistical coupling analysis (SCA) and molecular dynamics (MD) simulations. The coevolving residues identified by the SCA were found to form a network which encloses the active site completely. The MD simulations showed that these coevolving residues are involved in the correlated and anti-correlated motions. The correlated residues are within van der Waals contact and appear to maintain the active site architecture; the anti-correlated residues are mainly distributed on opposite sides of the catalytic cavity and coordinate the motions likely required for the substrate entry and product release. Our findings might have broad implications for proteins with the highly conserved (betaalpha)(8)-barrel in assessing the roles of amino-acids that are moderately conserved and not directly involved in the active site of the (beta/alpha)(8)-barrel. The results of this study could also provide useful information for further exploring the specific residue motions for the catalysis and protein design based on the (beta/alpha)(8)-barrel scaffold.

  1. Linking topography to tonotopy in the mouse auditory thalamocortical circuit

    DEFF Research Database (Denmark)

    Hackett, Troy A; Rinaldi Barkat, Tania; O'Brien, Barbara M J

    2011-01-01

    The mouse sensory neocortex is reported to lack several hallmark features of topographic organization such as ocular dominance and orientation columns in primary visual cortex or fine-scale tonotopy in primary auditory cortex (AI). Here, we re-examined the question of auditory functional topography...... the tonotopic axis in the slice produced an orderly shift of voltage-sensitive dye (VSD) signals along the AI tonotopic axis, demonstrating topography in the mouse thalamocortical circuit that is preserved in the slice. However, compared with BF maps of neuronal spiking activity, the topographic order...... of subthreshold VSD maps was reduced in layer IV and even further degraded in layer II/III. Therefore, the precision of AI topography varies according to the source and layer of the mapping signal. Our findings further bridge the gap between in vivo and in vitro approaches for the detailed cellular study...

  2. Conserved size and periodicity of pyramidal patches in layer 2 of medial/caudal entorhinal cortex.

    Science.gov (United States)

    Naumann, Robert K; Ray, Saikat; Prokop, Stefan; Las, Liora; Heppner, Frank L; Brecht, Michael

    2016-03-01

    To understand the structural basis of grid cell activity, we compare medial entorhinal cortex architecture in layer 2 across five mammalian species (Etruscan shrews, mice, rats, Egyptian fruit bats, and humans), bridging ∼100 million years of evolutionary diversity. Principal neurons in layer 2 are divided into two distinct cell types, pyramidal and stellate, based on morphology, immunoreactivity, and functional properties. We confirm the existence of patches of calbindin-positive pyramidal cells across these species, arranged periodically according to analyses techniques like spatial autocorrelation, grid scores, and modifiable areal unit analysis. In rodents, which show sustained theta oscillations in entorhinal cortex, cholinergic innervation targeted calbindin patches. In bats and humans, which only show intermittent entorhinal theta activity, cholinergic innervation avoided calbindin patches. The organization of calbindin-negative and calbindin-positive cells showed marked differences in entorhinal subregions of the human brain. Layer 2 of the rodent medial and the human caudal entorhinal cortex were structurally similar in that in both species patches of calbindin-positive pyramidal cells were superimposed on scattered stellate cells. The number of calbindin-positive neurons in a patch increased from ∼80 in Etruscan shrews to ∼800 in humans, only an ∼10-fold over a 20,000-fold difference in brain size. The relatively constant size of calbindin patches differs from cortical modules such as barrels, which scale with brain size. Thus, selective pressure appears to conserve the distribution of stellate and pyramidal cells, periodic arrangement of calbindin patches, and relatively constant neuron number in calbindin patches in medial/caudal entorhinal cortex. © 2015 The Authors. The Journal of Comparative Neurology Published by Wiley Periodicals, Inc.

  3. Conserved size and periodicity of pyramidal patches in layer 2 of medial/caudal entorhinal cortex

    Science.gov (United States)

    Naumann, Robert K.; Ray, Saikat; Prokop, Stefan; Las, Liora; Heppner, Frank L.

    2016-01-01

    ABSTRACT To understand the structural basis of grid cell activity, we compare medial entorhinal cortex architecture in layer 2 across five mammalian species (Etruscan shrews, mice, rats, Egyptian fruit bats, and humans), bridging ∼100 million years of evolutionary diversity. Principal neurons in layer 2 are divided into two distinct cell types, pyramidal and stellate, based on morphology, immunoreactivity, and functional properties. We confirm the existence of patches of calbindin‐positive pyramidal cells across these species, arranged periodically according to analyses techniques like spatial autocorrelation, grid scores, and modifiable areal unit analysis. In rodents, which show sustained theta oscillations in entorhinal cortex, cholinergic innervation targeted calbindin patches. In bats and humans, which only show intermittent entorhinal theta activity, cholinergic innervation avoided calbindin patches. The organization of calbindin‐negative and calbindin‐positive cells showed marked differences in entorhinal subregions of the human brain. Layer 2 of the rodent medial and the human caudal entorhinal cortex were structurally similar in that in both species patches of calbindin‐positive pyramidal cells were superimposed on scattered stellate cells. The number of calbindin‐positive neurons in a patch increased from ∼80 in Etruscan shrews to ∼800 in humans, only an ∼10‐fold over a 20,000‐fold difference in brain size. The relatively constant size of calbindin patches differs from cortical modules such as barrels, which scale with brain size. Thus, selective pressure appears to conserve the distribution of stellate and pyramidal cells, periodic arrangement of calbindin patches, and relatively constant neuron number in calbindin patches in medial/caudal entorhinal cortex. J. Comp. Neurol. 524:783–806, 2016. © 2015 The Authors. The Journal of Comparative Neurology Published by Wiley Periodicals, Inc. PMID:26223342

  4. PACAP decides neuronal laminar fate via PKA signaling in the developing cerebral cortex

    International Nuclear Information System (INIS)

    Ohtsuka, Masanari; Fukumitsu, Hidefumi; Furukawa, Shoei

    2008-01-01

    Laminar formation in the developing cerebral cortex requires the precisely regulated generation of phenotype-specified neurons. To test the possible involvement of pituitary adenylate cyclase-activating polypeptide (PACAP) in this formation, we investigated the effects of PACAP administered into the telencephalic ventricular space of 13.5-day-old mouse embryos. PACAP partially inhibited the proliferation of cortical progenitors and altered the position and gene-expression profiles of newly generated neurons otherwise expected for layer IV to those of neurons for the deeper layers, V and VI, of the cerebral cortex. The former and latter effects were seen only when the parent progenitor cells were exposed to PACAP in the later and in earlier G1 phase, respectively; and these effects were suppressed by co-treatment with a protein kinase A (PKA) inhibitor. These observations suggest that PACAP participates in the processes forming the neuronal laminas in the developing cortex via the intracellular PKA pathway

  5. Prediction of Propellant and Explosive Cook-Off for the 30-mm HEI-T and Raufoss MPLD-T Rounds Chambered in a Hot MK44 Barrel (Advanced Amphibious Assault Vehicle - AAAV

    National Research Council Canada - National Science Library

    Witherell, Mark

    2001-01-01

    ... interior ballistics program. Heat transfer between the barrel, projectile, and the environment during a simulated misfire event was modeled using the ABAQUS finite element program with the initial barrel temperature distribution...

  6. Result from systematic compilation of barrel bolt findings in S/KWU type PWRs in the context of computational analysis

    International Nuclear Information System (INIS)

    Kilian, R.; Devrient, B.; Koenig, G.; Stanislowski, M.; Widera, M.; Beusekom, R. van; Wermelinger, T.

    2015-01-01

    In 2005 intergranular stress corrosion cracking (IGSCC) of barrel bolts was observed in several S/KWU type PWRs. The bolts, known as star bolts, are made of SS type 316 Ti (German Material No. 1.4571 K) bars which are cold worked to adjust the required mechanical properties. This damage mechanism was so far less understood for PWR primary conditions. Therefore an extended joint research program was launched by AREVA GmbH and VGB e.V. to clarify the specific conditions which contributed to the observed findings on barrel bolts. A systematic analysis of the IGSCC affecting parameters as material, heats, environment and mechanical load was performed based on a plant data compilation from all six S/KWU PWRs with comparable core barrel design using barrel and baffle bolts made from type 316Ti. Using the outcome of this systematic data compilation additional computational fluid dynamics calculations in combination with radiolysis calculations were performed. The results showed that by a combination of reduced volume exchange by local flow conditions and radiolysis reactions forming oxidizing species as dissolved oxygen and/or hydrogen peroxide may locally affect the corrosion behavior of cold worked austenitic stainless steels. Therefore, small local volumes with oxidizing water chemistry conditions are assumed to lead to the IGSCC of cold worked type 316Ti. The comparison of the initial cold worked microstructure by TEM with the cold worked and in service irradiated microstructure (void formation, dislocation loop density, etc.) clearly reveals that neutron irradiation hardening in terms of IASCC (Irradiation-Assisted Stress Corrosion Cracking) is not the leading mechanism for these cases of barrel bolt cracking in the analyzed PWRs. (authors)

  7. Distinct timescales of population coding across cortex.

    Science.gov (United States)

    Runyan, Caroline A; Piasini, Eugenio; Panzeri, Stefano; Harvey, Christopher D

    2017-08-03

    The cortex represents information across widely varying timescales. For instance, sensory cortex encodes stimuli that fluctuate over few tens of milliseconds, whereas in association cortex behavioural choices can require the maintenance of information over seconds. However, it remains poorly understood whether diverse timescales result mostly from features intrinsic to individual neurons or from neuronal population activity. This question remains unanswered, because the timescales of coding in populations of neurons have not been studied extensively, and population codes have not been compared systematically across cortical regions. Here we show that population codes can be essential to achieve long coding timescales. Furthermore, we find that the properties of population codes differ between sensory and association cortices. We compared coding for sensory stimuli and behavioural choices in auditory cortex and posterior parietal cortex as mice performed a sound localization task. Auditory stimulus information was stronger in auditory cortex than in posterior parietal cortex, and both regions contained choice information. Although auditory cortex and posterior parietal cortex coded information by tiling in time neurons that were transiently informative for approximately 200 milliseconds, the areas had major differences in functional coupling between neurons, measured as activity correlations that could not be explained by task events. Coupling among posterior parietal cortex neurons was strong and extended over long time lags, whereas coupling among auditory cortex neurons was weak and short-lived. Stronger coupling in posterior parietal cortex led to a population code with long timescales and a representation of choice that remained consistent for approximately 1 second. In contrast, auditory cortex had a code with rapid fluctuations in stimulus and choice information over hundreds of milliseconds. Our results reveal that population codes differ across cortex

  8. Layer-specific excitation/inhibition balances during neuronal synchronization in the visual cortex.

    Science.gov (United States)

    Adesnik, Hillel

    2018-05-01

    Understanding the balance between synaptic excitation and inhibition in cortical circuits in the brain, and how this contributes to cortical rhythms, is fundamental to explaining information processing in the cortex. This study used cortical layer-specific optogenetic activation in mouse cortex to show that excitatory neurons in any cortical layer can drive powerful gamma rhythms, while inhibition balances excitation. The net impact of this is to keep activity within each layer in check, but simultaneously to promote the propagation of activity to downstream layers. The data show that rhythm-generating circuits exist in all principle layers of the cortex, and provide layer-specific balances of excitation and inhibition that affect the flow of information across the layers. Rhythmic activity can synchronize neural ensembles within and across cortical layers. While gamma band rhythmicity has been observed in all layers, the laminar sources and functional impacts of neuronal synchronization in the cortex remain incompletely understood. Here, layer-specific optogenetic stimulation demonstrates that populations of excitatory neurons in any cortical layer of the mouse's primary visual cortex are sufficient to powerfully entrain neuronal oscillations in the gamma band. Within each layer, inhibition balances excitation and keeps activity in check. Across layers, translaminar output overcomes inhibition and drives downstream firing. These data establish that rhythm-generating circuits exist in all principle layers of the cortex, but provide layer-specific balances of excitation and inhibition that may dynamically shape the flow of information through cortical circuits. These data might help explain how excitation/inhibition (E/I) balances across cortical layers shape information processing, and shed light on the diverse nature and functional impacts of cortical gamma rhythms. © 2018 The Authors. The Journal of Physiology © 2018 The Physiological Society.

  9. Cultured embryonic non-innervated mouse muzzle is capable of generating a whisker pattern.

    Science.gov (United States)

    Andrés, F L; Van Der Loos, H

    1983-01-01

    The whisker pattern on the muzzle of the mouse is mapped in the contralateral parietal neocortex, each whisker follicle projecting to its own multineuronal unit ('barrel'). To determine the role, if any, of the peripheral innervation in the establishment of the vibrissal array, we cultured non-innervated prospective whiskerpads from 9- and 10-day-old embryos, mostly on chorioallantoic membrane. The results show that skin, alone, is capable of generating the whisker pattern, thus adducing a strong argument for the hypothesis that the central brain maps have their origin in the periphery. Copyright © 1983. Published by Elsevier Ltd.

  10. The distribution of infered energetic electron loss with respect to plasmapause location: BARREL results.

    Science.gov (United States)

    Halford, A. J.; Malaspina, D.; Sibeck, D. G.

    2017-12-01

    One of the long outstanding challenges of understanding the inner magnetosphere is accurately describing radiation belt dynamics. This enterprise can seem daunting as many have stated: "if you've seen one storm you've seen one storm". And although much progress has been made over the last half century since the discovery of the radiation belts, there is still ongoing debate about the relative importance of different loss and source mechanisms. Here we will consider one part of radiation belt dynamics, the loss of electrons ( 30 keV to MeV) to the upper atmosphere and endeavor to identify the relative importance of the different loss mechanisms. As demonstrated in often used cartoon diagrams, and previous studies, many radiation belt loss mechanisms such as chorus, hiss, and EMIC waves are thought to have specific MLT and L dependencies as well as dependence on geomagnetic conditions. Many of these loss mechanisms are identifiable through the energies and time scales in which they precipitate electrons. Thus we expect that the observed electron precipiation should follow similar MLT and L patterns as what caused the loss and not show something completely unexpected such as Atlantis rising out of the Columbia River. Here we will examine the location and geomagnetic conditions under which the Balloon Array for Relativistic Radiation Belt Electron Loss (BARREL) inferred radiation belt electron precipitation. The BARREL mission consisted of 4 campaigns, two in Antarctica and 2 in Sweden, for a total of 55 launches. The flights conducted in Antarctica took advantage of the circumpolar winds allowing for the payloads to cross a range of L-values from L > 2.5 onto open field lines, while the Swedish campaigns were held during turn around where the balloons stayed near L = 5.8. We will present the distribution of precipitation with respect to L and MLT as well as with respect to the boundary of the plasmapause as new work has shown that this boundary clearly separates

  11. Lipid bilayer-bound conformation of an integral membrane beta barrel protein by multidimensional MAS NMR

    International Nuclear Information System (INIS)

    Eddy, Matthew T.; Su, Yongchao; Silvers, Robert; Andreas, Loren; Clark, Lindsay; Wagner, Gerhard; Pintacuda, Guido; Emsley, Lyndon; Griffin, Robert G.

    2015-01-01

    The human voltage dependent anion channel 1 (VDAC) is a 32 kDa β-barrel integral membrane protein that controls the transport of ions across the outer mitochondrial membrane. Despite the determination of VDAC solution and diffraction structures, a structural basis for the mechanism of its function is not yet fully understood. Biophysical studies suggest VDAC requires a lipid bilayer to achieve full function, motivating the need for atomic resolution structural information of VDAC in a membrane environment. Here we report an essential step toward that goal: extensive assignments of backbone and side chain resonances for VDAC in DMPC lipid bilayers via magic angle spinning nuclear magnetic resonance (MAS NMR). VDAC reconstituted into DMPC lipid bilayers spontaneously forms two-dimensional lipid crystals, showing remarkable spectral resolution (0.5–0.3 ppm for 13 C line widths and <0.5 ppm 15 N line widths at 750 MHz). In addition to the benefits of working in a lipid bilayer, several distinct advantages are observed with the lipid crystalline preparation. First, the strong signals and sharp line widths facilitated extensive NMR resonance assignments for an integral membrane β-barrel protein in lipid bilayers by MAS NMR. Second, a large number of residues in loop regions were readily observed and assigned, which can be challenging in detergent-solubilized membrane proteins where loop regions are often not detected due to line broadening from conformational exchange. Third, complete backbone and side chain chemical shift assignments could be obtained for the first 25 residues, which comprise the functionally important N-terminus. The reported assignments allow us to compare predicted torsion angles for VDAC prepared in DMPC 2D lipid crystals, DMPC liposomes, and LDAO-solubilized samples to address the possible effects of the membrane mimetic environment on the conformation of the protein. Concluding, we discuss the strengths and weaknesses of the reported

  12. The binding cavity of mouse major urinary protein is optimised for a variety of ligand binding modes

    Energy Technology Data Exchange (ETDEWEB)

    Pertinhez, Thelma A.; Ferrari, Elena; Casali, Emanuela [Department of Experimental Medicine, University of Parma, Via Volturno, 39, 43100 Parma (Italy); Patel, Jital A. [Department of Chemistry, University of Oxford, Inorganic Chemistry Laboratory, South Parks Road, Oxford OX1 3QR (United Kingdom); Spisni, Alberto, E-mail: alberto.spisni@unipr.it [Department of Experimental Medicine, University of Parma, Via Volturno, 39, 43100 Parma (Italy); Smith, Lorna J., E-mail: lorna.smith@chem.ox.ac.uk [Department of Chemistry, University of Oxford, Inorganic Chemistry Laboratory, South Parks Road, Oxford OX1 3QR (United Kingdom)

    2009-12-25

    {sup 15}N and {sup 1}HN chemical shift data and {sup 15}N relaxation studies have been used to characterise the binding of N-phenyl-naphthylamine (NPN) to mouse major urinary protein (MUP). NPN binds in the {beta}-barrel cavity of MUP, hydrogen bonding to Tyr120 and making extensive non-bonded contacts with hydrophobic side chains. In contrast to the natural pheromone 2-sec-butyl-4,5-dihydrothiazole, NPN binding gives no change to the overall mobility of the protein backbone of MUP. Comparison with 11 different ligands that bind to MUP shows a range of binding modes involving 16 different residues in the {beta}-barrel cavity. These finding justify why MUP is able to adapt to allow for many successful binding partners.

  13. Prenatal cocaine exposure decreases parvalbumin-immunoreactive neurons and GABA-to-projection neuron ratio in the medial prefrontal cortex.

    Science.gov (United States)

    McCarthy, Deirdre M; Bhide, Pradeep G

    2012-01-01

    Cocaine abuse during pregnancy produces harmful effects not only on the mother but also on the unborn child. The neurotransmitters dopamine and serotonin are known as the principal targets of the action of cocaine in the fetal and postnatal brain. However, recent evidence suggests that cocaine can impair cerebral cortical GABA neuron development and function. We sought to analyze the effects of prenatal cocaine exposure on the number and distribution of GABA and projection neurons (inhibitory interneurons and excitatory output neurons, respectively) in the mouse cerebral cortex. We found that the prenatal cocaine exposure decreased GABA neuron numbers and GABA-to-projection neuron ratio in the medial prefrontal cortex of 60-day-old mice. The neighboring prefrontal cortex did not show significant changes in either of these measures. However, there was a significant increase in projection neuron numbers in the prefrontal cortex but not in the medial prefrontal cortex. Thus, the effects of cocaine on GABA and projection neurons appear to be cortical region specific. The population of parvalbumin-immunoreactive GABA neurons was decreased in the medial prefrontal cortex following the prenatal cocaine exposure. The cocaine exposure also delayed the developmental decline in the volume of the medial prefrontal cortex. Thus, prenatal cocaine exposure produced persisting and region-specific effects on cortical cytoarchitecture and impaired the physiological balance between excitatory and inhibitory neurotransmission. These structural changes may underlie the electrophysiological and behavioral effects of prenatal cocaine exposure observed in animal models and human subjects. Copyright © 2012 S. Karger AG, Basel.

  14. Motor learning in animal models of Parkinson's disease: Aberrant synaptic plasticity in the motor cortex.

    Science.gov (United States)

    Xu, Tonghui; Wang, Shaofang; Lalchandani, Rupa R; Ding, Jun B

    2017-04-01

    In Parkinson's disease (PD), dopamine depletion causes major changes in the brain, resulting in the typical cardinal motor features of the disease. PD neuropathology has been restricted to postmortem examinations, which are limited to only a single time of PD progression. Models of PD in which dopamine tone in the brain is chemically or physically disrupted are valuable tools in understanding the mechanisms of the disease. The basal ganglia have been well studied in the context of PD, and circuit changes in response to dopamine loss have been linked to the motor dysfunctions in PD. However, the etiology of the cognitive dysfunctions that are comorbid in PD patients has remained unclear until now. In this article, we review recent studies exploring how dopamine depletion affects the motor cortex at the synaptic level. In particular, we highlight our recent findings on abnormal spine dynamics in the motor cortex of PD mouse models through in vivo time-lapse imaging and motor skill behavior assays. In combination with previous studies, a role of the motor cortex in skill learning and the impairment of this ability with the loss of dopamine are becoming more apparent. Taken together, we conclude with a discussion on the potential role for the motor cortex in PD, with the possibility of targeting the motor cortex for future PD therapeutics. © 2017 International Parkinson and Movement Disorder Society. © 2017 International Parkinson and Movement Disorder Society.

  15. An FPGA-based Sampling-ADC readout for the crystal barrel calorimeter

    Energy Technology Data Exchange (ETDEWEB)

    Muellers, Johannes [Helmholtz-Institut fuer Strahlen- und Kernphysik, Bonn (Germany); Marciniewski, Pawel [Angstroemlaboratoriet, Uppsala (Sweden); Collaboration: CBELSA/TAPS-Collaboration

    2016-07-01

    The CBELSA/TAPS experiment at the electron accelerator ELSA (Bonn) investigates the photoproduction of mesons off protons and neutrons. The Crystal Barrel Calorimeter has been upgraded replacing its photodiode readout by APDs, which allows the integration of the calorimeter into the first level trigger. Since the possible DAQ rate is currently limited by the digitization stage (LeCroy QDC1885F) to ∼ 2 kHz, the implementation of a new Sampling-ADC (SADC) readout is the second important step in the upgrade of the detector system. Based on the 64-channel PANDA-SADC, the design was modified, adapting it to the needs of the CBELSA/TAPS experiment. The CB-SADC offers 64 channels in one NIM module with up to 14 bit rate at 125 MHz, accompanied by a modular analog input stage and power supply. Data processing and reduction are realized with Kintex7 FPGAs. Readout is possible via gigabit ethernet links. Using an FPGA provides a multitude of possibilities for online feature extraction, such as the determination of the energy deposited in the crystal, TDC capabilities and pile-up detection and recovery. The SADC development is discussed, and first measurements performed in comparison to the presently used LeCroy QDC are presented.

  16. A new avalanche photo diode based readout for the crystal barrel calorimeter

    Energy Technology Data Exchange (ETDEWEB)

    Urban, Martin [Helmholtz-Institut fuer Strahlen- und Kernphysik, Nussallee 14-16, 53115 Bonn (Germany); Collaboration: CBELSA/TAPS-Collaboration

    2015-07-01

    The CBELSA/TAPS experiment at ELSA has proven successful in the measurement of double polarization observables in meson photoproduction off protons and neutrons. To be able to measure purely neutral reactions on a polarized neutron target with high efficiency, the main calorimeter consisting of 1320 CsI(Tl) crystals has to be integrated into the first level trigger. Key requirement to achieve this goal is an exchange of the existing PIN photo diode by a new avalanche photo diode (APD) readout. The main advantage of the new readout system is that it will provide timing information which allows a fast trigger signal. The energy resolution will remain compatible to the previous system. Besides the development of automated test routines for the front end electronics, the characterization of all APDs was successfully accomplished in Bonn. After tests with a 3 x 3 CsI(Tl) crystal matrix at the tagged photon beam facilities at ELSA and MAMI the first half of the Crystal Barrel was upgraded in 2014. This talk shows the result of the latest test measurements including the gain stabilization of the new APD readout electronics and presents the progress of the ongoing upgrade.

  17. Barrel time-of-flight detector for the PANDA experiment at FAIR

    Science.gov (United States)

    Gruber, L.; Brunner, S. E.; Marton, J.; Orth, H.; Suzuki, K.; PANDA Tof Group

    2016-07-01

    The barrel time-of-flight detector for the PANDA experiment at FAIR is foreseen as a Scintillator Tile (SciTil) Hodoscope based on several thousand small plastic scintillator tiles read-out with directly attached Silicon Photomultipliers (SiPMs). The main tasks of the system are an accurate determination of the time origin of particle tracks to avoid event mixing at high collision rates, relative time-of-flight measurements as well as particle identification in the low momentum regime. The main requirements are the use of a minimum material amount and a time resolution of σ < 100 ps. We have performed extensive optimization studies and prototype tests to prove the feasibility of the SciTil design and finalize the R&D phase. In a 2.7 GeV/c proton beam at Forschungszentrum Jülich a time resolution of about 80 ps has been achieved using SiPMs from KETEK and Hamamatsu with an active area of 3 × 3mm2. Employing the Digital Photon Counter from Philips a time resolution of about 30 ps has been reached.

  18. Slice Test Results of the ATLAS Barrel Muon Level-1 Trigger

    CERN Document Server

    Aielli, G; Alviggi, M G; Bocci, V; Brambilla, Elena; Canale, V; Caprio, M A; Cardarelli, R; Cataldi, G; De Asmundis, R; Della Volpe, D; Di Ciaccio, A; Di Simone, A; Distante, L; Gorini, E; Grancagnolo, F; Iengo, P; Nisati, A; Pastore, F; Patricelli, S; Perrino, R; Petrolo, E; Primavera, M; Salamon, A; Santonico, R; Sekhniaidze, G; Severi, M; Spagnolo, S; Vari, R; Veneziano, Stefano; 9th Workshop On Electronics For LHC Experiments - LECC 2003

    2003-01-01

    The muon spectrometer of the ATLAS experiment makes use of the Resistive Plate Chambers detectors for particle tracking in the barrel region. The level-1 muon trigger system has to measure and discriminate muon transverse momentum, perform a fast and coarse tracking of the muon candidates, associate them to the bunch crossing corresponding to the event of interest, measure the second coordinate in the non-bending projection. The on-detector electronics first collects front-end signals coming from the two inner RPC stations on the low-pT PAD boards, each one covering a region of DetaxDphi=0.2x0.2, and hosting four Coincidence Matrix ASICs. Each CMA performs the low-pT trigger algorithm and data readout on a region of DetaxDphi=0.2x0.1. Data coming from the four CMAs are assembled by the low-pT PAD logic. Each low-pT PAD board sends data to the corresponding high-pT PAD boards, located on the outer RPC station. Four CMA on each board make use of the low-pT trigger result and of the front-end signals coming from...

  19. A level-1 track trigger for CMS with double stack detectors and long barrel approach

    International Nuclear Information System (INIS)

    Salvati, E

    2012-01-01

    The upgrade of the LHC machine is planned to deliver luminosities 5 to 10 times larger than the design one of 1 × 10 34 cm −2 s −1 . A novel tracking system for the CMS experiment must be designed and built. One main aspect of the current activities consists in understanding the capabilities that different designs such a tracker would have to provide for the Level 1 hardware trigger to complement the muon and calorimeter information. Data rate reduction at hardware level consists in both reducing multiple hits from a single track and rejection of low p t tracks. Pattern-based hit correlation of properly built clusters of hits would provide quality Level 1 primitives to the hardware trigger. These can be combined together in a projective geometry to perform a rough tracking to be implemented online, returning rough p t , direction, and vertex information for a candidate track. The benchmark results from simulations within the official CMS framework are presented for one particular layout based on barrel trigger layers, emphasizing the flexibility of this tool for the design and test of different tracking strategies at level 1 to be compared with the developments in trigger architectures implementation.

  20. Bad Apples, Bad Barrel: Exploring Institutional Responses to Child Sexual Abuse by Catholic Clergy in Australia

    Directory of Open Access Journals (Sweden)

    Jodi Death

    2015-07-01

    Full Text Available This paper considers constructions of institutional culture and power in the cover-up of child sexual abuse (CSA by clergy in the Roman Catholic Church of Australia. The issue of cover-up has previously been considered in international inquiries as an institutional failing that has caused significant harm to victims of CSA by Catholic Clergy. Evidence given by select representatives of the Catholic Church in two government inquiries into institutional abuse carried out in Australia is considered here. This evidence suggests that, where cover-up has occurred, it has been reliant on the abuse of institutional power and resulted in direct emotional, psychological and spiritual harm to victims of abuse. Despite international recognition of cover-up as institutional abuse, evidence presented by Roman Catholic Representatives to the Victorian Inquiry denied there was an institutionalised cover-up. Responding to this evidence, this paper queries whether the primary foundation of cover-up conforms to the ‘bad apple theory’ in that it relates only to a few individuals, or the ‘bad barrel theory’ of institutional structure and culture.

  1. ATLAS Barrel Hadron Calorimeter: general manufacturing concepts for 300000 absorber plates mass production

    International Nuclear Information System (INIS)

    Alikov, B.A.; Budagov, Yu.A.; Bylinkin, P.M

    1998-01-01

    We summarize a 4-year (1994-1997) experience of design and research efforts which led us to the solution of 2 important tasks of a principal significance for precision assembly of one of major elements of ATLAS, - its Hadron Barrel Tile Calorimeter. These tasks were: - to develop the high tolerances (50-100 microns) technology for about 300000 units of calorimeter nuclear absorber plates mass production, - to choose the best manufacturer(s) able to satisfy shop drawings demands in a reasonable balance with some other significant criteria: production period, price acceptable geography location (transport expenses), available storage area and access ways, reliable quality control etc. For the best absorbers producers our final choice was the TATRA PLANT (Czech Republic) for 1.6 m long plates stamping (40800 units) with Argonne punching die and the MINSK TRACTOR PLANT (Belarus Republic) for smaller size plates stamping (about 240000 units). We excl