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Sample records for monroe mi lawrence

  1. Congenital constriction of the foramen of Monro

    International Nuclear Information System (INIS)

    Marions, O.; Boethius, J.

    1986-01-01

    We report two cases of hydrocephalus in adults. The radiological investigations and direct inspection during surgery in one of the cases indicate that the hydrocephalus is caused in both cases by a benign stricture in the region of the foramen of Monro and that this constriction is congenital. This origin of hydrocephalus has not been reported previously in adults. (orig.)

  2. Alexander Monro Tertius and his works on hydrocephalus.

    Science.gov (United States)

    Tubbs, R Shane; Tubbs, Isaiah; Loukas, Marios; Oakes, W Jerry

    2015-03-01

    The Monros of the University of Edinburgh reigned over anatomy instruction for over a century. The last of these men, Monro Tertius, was the weaker teacher of the family but still contributed to the anatomical and surgical literature. Herein, we describe the life of Alexander Monro Tertius and his writings, particularly on childhood hydrocephalus. Monro Tertius will not be remembered as a great anatomist or teacher. However, he collected and published important books on his observations and those of others on hydrocephalus. These texts contained the knowledge of his era on this topic.

  3. Lawrence and his laboratory

    International Nuclear Information System (INIS)

    Hellbron, J.L.; Seidel, R.W.

    1989-01-01

    The birthplace of nuclear chemistry and nuclear medicine is the subject of this study of the Radiation Laboratory in Berkeley, California, where Ernest Lawrence used local and national technological, economic, and manpower resources to build the cyclotron

  4. Lawrence, Prof. Ernest Orlando

    Indian Academy of Sciences (India)

    Home; Fellowship. Fellow Profile. Elected: 1954 Honorary. Lawrence, Prof. Ernest Orlando Nobel Laureate (Physics) - 1939. Date of birth: 8 August 1901. Date of death: 27 August 1958. YouTube; Twitter; Facebook; Blog. Academy News. IAS Logo. 29th Mid-year meeting. Posted on 19 January 2018. The 29th Mid-year ...

  5. E.O. Lawrence

    Science.gov (United States)

    period in his life when he thought that his discoveries in the field of high explosives would make war so pleasure of a visit to Sweden. I hope that we can in some measure compensate for that loss. I hope too disintegration. The picture has been sketchy; yet I hope it has indicated the versatility of his ideas. lawrence

  6. Lawrence v. Texas

    OpenAIRE

    Kristan, Andrej

    2014-01-01

    Članek predstavlja nedavno rešeni ameriški primer Lawrence proti Texasu, ki bo v ustavnopravno kroniko zapisan s tremi poudarki. 1. je bilo na Vrhovnem sodišču ZDA v tem primeru izpostavljeno, da standard vrednot »zahodne civilizacije« postavlja Evropsko sodišče za človekove pravice. Ta standard se v ZDA prenaša s odločitvijo vrhovnega sodišča, da imajo homoseksualne osebe pravico do zasebnosti. 2. se s tem primerom postavljajo temelji inkorporacije mednarodnega prava človekovih pravic v amer...

  7. Fermi, Heisenberg y Lawrence

    Directory of Open Access Journals (Sweden)

    Ynduráin, Francisco J.

    2002-01-01

    Full Text Available Not available

    Los azares de las onomásticas hacen coincidir en este año el centenario del nacimiento de tres de los más grandes físicos del siglo XX. Dos de ellos, Fermi y Heisenberg, dejaron una marca fundamental en la ciencia (ambos, pero sobre todo el segundo y, el primero, también en la tecnología. Lawrence, indudablemente de un nivel inferior al de los otros dos, estuvo sin embargo en el origen de uno de los desarrollos tecnológicos que han sido básicos para la exploración del universo subnuclear en la segunda mitad del siglo que ha terminado hace poco, el de los aceleradores de partículas.

  8. Russia’s Monroe Doctrine: peacekeeping, peacemaking, or imperial outreach?

    Directory of Open Access Journals (Sweden)

    Teresa Rakowska-Harmstone

    2014-06-01

    Full Text Available The article discusses the important changes in the Russian foreign policy doctrines that occurred in the beginnings of the 1990s, after the collapse of the Soviet Union. The Author argues that the officially claimed devotion to peacemaking and peacekeeping are in fact manifestations of the Russian imperial outreach. The model of international relations promoted by Moscow in fact resembles the American 19th century Monroe Doctrine. Thus, the foreign policy doctrine and the potential national conflicts in the post-Soviet territory may become triggers for Russian actions aiming at restoring the Russian Empire.

  9. The remarkable medical lineage of the Monro family: contributions of Alexander primus, secundus, and tertius.

    Science.gov (United States)

    Wu, Osmond C; Manjila, Sunil; Malakooti, Nima; Cohen, Alan R

    2012-06-01

    Among the families that have influenced the development of modern medicine into what it is today, the Monro lineage stands as one of the most notable. Alexander Monro primus (1697-1767) was the first of 3 generations with the same name, a dynasty that spanned 126 years occupying the Chair of Anatomy one after the other at the University of Edinburgh. After becoming Professor of Anatomy at the University of Edinburgh in 1719, Monro primus played a principal role in the establishment of the University of Edinburgh School of Medicine and the Edinburgh Royal Infirmary. In 1726, he published The Anatomy of the Humane Bones, of which 8 editions were printed during his lifetime. His son, Alexander Monro secundus (1733-1817), arguably the most notable of the 3 men, succeeded him as Professor of Anatomy. A highly regarded lecturer and anatomist, Monro secundus studied under many great physicians, including William Hunter and Johann Friedrich Meckel the Elder, and was also teacher to other well-known figures at the time, such as Joseph Black and Thomas Trotter. His most notable contributions include his work with the lymphatic system, the interventricular foramen (of Monro), and the Monro-Kellie doctrine. Alexander Monro tertius (1773-1859), the last of the dynasty, also succeeded his father as Professor of Anatomy. His work included insights into abdominal aortic aneurysms and the anatomy of the genitourinary system. The prominent association of the Monro family with the University of Edinburgh and the effects of a tenured professorship under the concept of "Ad vitam aut culpam" over successive generations are also described. To the best of the authors' knowledge, this historical review of the Monro family is among the few published in neurosurgical literature. A vivid historical overview of the medical contributions of the most famous and influential dynasty of physicians in Edinburgh at that time is provided, with relevant excerpts from original publications.

  10. Enlarged thalamostriate vein causing unilateral Monro foramen obstruction. Case report.

    Science.gov (United States)

    Leonardo, Jody; Grand, Walter

    2009-06-01

    Causes of unilateral hydrocephalus resulting from an obstruction at the Monro foramen include foraminal atresia, tumors, gliosis, contralateral shunting, and infectious and inflammatory conditions. However, few reports in the literature cite vascular lesions as the cause of the obstruction. To their knowledge, the authors present the first report of unilateral hydrocephalus occurring due to an abnormally enlarged thalamostriate vein independent of an arteriovenous malformation or developmental venous angioma. The condition was treated successfully by endoscopic septum pellucidum fenestration. A 28-year-old man was referred for evaluation due to a 10-year history of chronic headaches that worsened in severity over the past year. A CT scan of the head revealed unilateral right ventricular dilation. Cranial MR imaging with and without contrast administration showed a dilated right thalamostriate-internal vein complex without any evidence of associated arteriovenous malformation or venous angioma. Endoscopic exploration of the right lateral ventricle showed an enlarged subependymal thalamostriate vein obstructing the Monro foramen. An endoscopic fenestration of the septum pellucidum was performed, resulting in alleviation of the patient's symptoms. Abnormally enlarged venous structures may cause obstructive unilateral hydrocephalus and can be a rare cause of chronic, intermittent headaches in adults. Endoscopic fenestration of the septum pellucidum is an effective treatment.

  11. Suicidal traits in Marilyn Monroe's Fragments: an LIWC analysis.

    Science.gov (United States)

    Fernández-Cabana, M; García-Caballero, A; Alves-Pérez, M T; García-García, M J; Mateos, R

    2013-01-01

    Linguistic inquiry and word count (LIWC), a computerized method for text analysis, is often used to examine suicide writings in order to characterize the quantitative linguistic features of suicidal texts. To analyze texts compiled in Marilyn Monroe's Fragments using LIWC, in order to explore the use of different linguistic categories in her narrative over the years. Selected texts were grouped into four periods of similar word count and processed with LIWC. Spearman's rank correlation was used to assess changes in language use across the documents over time. The Kruskal-Wallis test was applied to compare means between periods and for each of the 80 LIWC output scores. Significant differences (p < .05) were found in 11 categories, the most relevant being a progressive decrease in the use of negative emotion words, a reduction in the use of long words in the third period, and an increase in the proportion of personal pronouns used as Monroe approached the time of her death. The consistently elevated usage of first-person personal singular pronouns and the consistently diminished usage of first-person personal plural pronouns are in line with previous studies linking this pattern with a low level of social integration, which has been related to suicide according to different theories.

  12. Neuroendoscopic treatment of idiopathic occlusion of unilateral foramen of Monro presenting as chronic headache

    Directory of Open Access Journals (Sweden)

    Dhaval Shukla

    2016-01-01

    Full Text Available Asymmetric ventriculomegly due to idiopathic occlusion of the foramen of Monro is rare. Such patients present with clinical features of raised intracranial pressure (ICP. Presentation as chronic headache has not been previously described. In the absence of raised ICP, pursuing surgical treatment raises a clinical dilemma as the headache may be a primary headache with no improvement after surgery. A 21-year-old woman presented with chronic headache. She was found to have asymmetric ventriculomegaly due to the occlusion of the foramen of Monro. She underwent endoscopic septostomy and widening of the foramen of Monro. Her headache subsided after surgery. At 15 months of follow-up, she was free from headache without medications. Unilateral occlusion of the foramen of Monro can present with asymmetric ventriculomegaly resulting in chronic headache. Though the symptoms of raised ICP may not be present, still endoscopic relief of ventriculomegaly leads to cure of headache.

  13. Endoscope-assisted resection of cavernous angioma at the foramen of Monro: a case report

    OpenAIRE

    Matsumoto, Yuji; Kurozumi, Kazuhiko; Shimazu, Yousuke; Ichikawa, Tomotsugu; Date, Isao

    2016-01-01

    Introduction Intraventricular cavernous angiomas are rare pathological entities, and those located at the foramen of Monro are even rarer. We herein present a case of cavernous angioma at the foramen of Monro that was successfully treated by neuroendoscope-assisted surgical removal, and review the relevant literature. Case presentation A 65-year-old woman had experienced headache and vomiting for 10?days before admission to another hospital. Magnetic resonance imaging (MRI) showed a mass at t...

  14. St. Lawrence action plan meter

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1992-12-31

    The purpose of this bulletin is to report on the progress achieved under the St. Lawrence Action Plan. Under each of the Action Plan`s five objectives, it outlines environmental management indicators which identify actions taken and shows the results. This report presents the data collected in late August 1992 on the activities carried out by all partners of both governments involved in SLAP. The objectives examined in the bulletin are: to reduce by 90% the liquid toxic waste discharged by the 50 plants targeted for priority action; to prepare remediation plans for contaminated federal sites and restore wetlands; to conserve 5000 additional hectares of habitat and create a marine park; to develop and implement recovery plans for mammals, fish, birds, amphibians, reptiles, and plants; and to determine the state of the St. Lawrence River.

  15. LAWRENCE RADIATION LABORATORY COUNTING HANDBOOK

    Energy Technology Data Exchange (ETDEWEB)

    Group, Nuclear Instrumentation

    1966-10-01

    The Counting Handbook is a compilation of operational techniques and performance specifications on counting equipment in use at the Lawrence Radiation Laboratory, Berkeley. Counting notes have been written from the viewpoint of the user rather than that of the designer or maintenance man. The only maintenance instructions that have been included are those that can easily be performed by the experimenter to assure that the equipment is operating properly.

  16. Endoscope-assisted resection of cavernous angioma at the foramen of Monro: a case report.

    Science.gov (United States)

    Matsumoto, Yuji; Kurozumi, Kazuhiko; Shimazu, Yousuke; Ichikawa, Tomotsugu; Date, Isao

    2016-01-01

    Intraventricular cavernous angiomas are rare pathological entities, and those located at the foramen of Monro are even rarer. We herein present a case of cavernous angioma at the foramen of Monro that was successfully treated by neuroendoscope-assisted surgical removal, and review the relevant literature. A 65-year-old woman had experienced headache and vomiting for 10 days before admission to another hospital. Magnetic resonance imaging (MRI) showed a mass at the foramen of Monro, and obstructive hydrocephalus of both lateral ventricles. The patient was then referred to our hospital. Neurological examination on admission to our hospital showed memory disturbance (Mini-Mental State Examination 20/30) and wide-based gait. A cavernous angioma at the foramen of Monro was diagnosed based on the typical popcorn-like appearance of the lesion on MRI. The lesion was completely removed by neuroendoscope-assisted transcortical surgery with the Viewsite Brain Access System (Vycor Medical Inc., Boca Raton, FL), leading to a reduction in the size of the ventricles. The resected mass was histologically confirmed to be cavernous angioma. The patient's symptoms resolved immediately and there were no postoperative complications. Minimally invasive neuroendoscope-assisted surgery was used to successfully treat a cavernous angioma at the foramen of Monro.

  17. T. E. Lawrence: Theorist and Campaign Planner

    Science.gov (United States)

    1992-05-12

    honors in history, Lawrence’s curiosity lead him to the works of Carl von Clausewitz, Henri Jomini. Karl von Willisen, Rudolf von Caemmerer, Helmut von...113. M. J. Steiner . Inside Pan-Arabia. (Chicago: Herxiricks House, 1947), Chap 7. 114. T. E. Lawrence, "The Evolution of a Revolt," p65. 115. T. E...Unity. New York: Devin-Adair, 1958. Steiner . M. J. Inside Pan-Arabia. Chicago: Hendricks House. 1947. Thomas, Lowell. With Lawrence in Arabia. New York

  18. James Monroe High School Proyecto Nuevos Horizontes, 1986-1987. OEA Evaluation Report.

    Science.gov (United States)

    Martinez, Ana L.; And Others

    In its second year of Title VII funding, James Monroe High Schools's Proyecto Nuevos Horizontes (Project New Horizons) served 344 limited-English-speaking recent arrivals from Latin America and the Caribbean, in grades 9 through 12. The program has built on the strengths of the high school's extensive computer-assisted instruction (CAI) program,…

  19. James Monroe High School Proyecto Nuevos Horizontes, 1985-1986. OEA Evaluation Report.

    Science.gov (United States)

    New York City Board of Education, Brooklyn. Office of Educational Assessment.

    Proyecto Nuevos Horizontes, a 3-year Title VII-funded bilingual education program, serves 287 Spanish speaking students at James Monroe High School (Bronx, New York). This report evaluates the project's first year of operation, 1985-86. The report contains an introduction describing the school and project goals; information on student…

  20. Nuevos Horizontes, James Monroe High School, 1987-1988. Evaluation Section Report. OREA Report.

    Science.gov (United States)

    Berney, Tomi D.; Lista, Carlos

    Proyecto Nuevos Horizontes (Project New Horizons) at James Monroe High School (New York City) served 328 students of limited English proficiency (LEP) in grades 9-12 during the final year of a 3-year funding cycle. The project's purpose was to build on the strengths of the school's extensive computer-assisted instructional program in order to…

  1. Contrasting Models of Posttraumatic Stress Disorder: Reply to Monroe and Mineka (2008)

    Science.gov (United States)

    Berntsen, Dorthe; Rubin, David C.; Bohni, Malene Klindt

    2008-01-01

    The authors address the 4 main points in S. M. Monroe and S. Mineka's comment. First, the authors show that the Diagnostic and Statistical Manual of Mental Disorders (4th ed., text rev.; American Psychiatric Association, 2000) posttraumatic stress disorder (PTSD) diagnosis includes an etiology and that it is based on a theoretical model with a…

  2. High-Dimensional Exploratory Item Factor Analysis by a Metropolis-Hastings Robbins-Monro Algorithm

    Science.gov (United States)

    Cai, Li

    2010-01-01

    A Metropolis-Hastings Robbins-Monro (MH-RM) algorithm for high-dimensional maximum marginal likelihood exploratory item factor analysis is proposed. The sequence of estimates from the MH-RM algorithm converges with probability one to the maximum likelihood solution. Details on the computer implementation of this algorithm are provided. The…

  3. Endoscopic resection of cavernoma of foramen of Monro in a patient with familial multiple cavernomatosis.

    Science.gov (United States)

    Prat, Ricardo; Galeano, Inmaculada

    2008-09-01

    Intraventricular cavernomas are extremely infrequent and only 11 cases of cavernous hemangioma to occur at the foramen of Monro have been reported in the literature. This 56 years old patient was admitted with progressive and intractable headache of 10 days of evolution. He was known to suffer familial multiple cavernomatosis. Magnetic resonance imaging (MRI), revealed obstructive hydrocephalus due to a cavernoma located in the area of the left foramen of Monro. Under neuronavigation guidance, complete endoscopic resection of the cavernoma was performed and normal ventricular size achieved. The patient experienced transient recent memory loss that resolved within a month after surgery. In the literature attempted endoscopic resection is reported to be abandoned due to bleeding and ineffectiveness of piecemeal endoscopic resection. In this case, the multiplicity of the lesions made it advisable to resect the lesion endoscopically, to avoid an open procedure in a patient with multiple potentially surgical lesions. Endoscopic resection was uneventful with easy control of bleeding with irrigation, suction, and bipolar coagulation despite dense vascular appearance of the lesion. During the procedure, precise visualization of the vascular structures around the foramen of Monro allowed complete resection with satisfactory control of the instruments. To the best of the authors' knowledge, this is the first published cavernoma of foramen of Monro successfully resected using an endoscopic approach.

  4. Early days in the Lawrence Laboratory

    International Nuclear Information System (INIS)

    McMillan, E.M.

    1976-10-01

    Events at the Lawrence Radiation Laboratory at Berkeley to the end of 1940 are recalled. Radiation detection, discovery of new isotopes and elements, and accelerators are among the subjects included. 29 photographs

  5. Uma política para o continente - reinterpretando a Doutrina Monroe

    Directory of Open Access Journals (Sweden)

    Carlos Gustavo Poggio Teixeira

    2014-12-01

    Full Text Available A declaração de novembro de 2013 do Secretário de Estado dos Estados Unidos, John Kerry, de que "a era da Doutrina Monroe terminou" é uma excelente oportunidade para rediscutir essa que é considerada um dos princípios cardeais da política externa norte-americana para a América Latina desde a independência dos Estados latino-americanos. O presente artigo pretende jogar luz em um aspecto frequentemente menosprezado pelas análises acerca da Doutrina Monroe, qual seja, o fato de que tal doutrina, longe de ser uma política concreta para todo o continente, tinha duas facetas bastante distintas tanto do ponto vista retórico como político. Através do exame principalmente de bibliografia produzida nos Estados Unidos, em especial daquelas que tiveram acesso a fontes primárias, o artigo demonstra que a Doutrina Monroe tinha um caráter claramente caribenho - e não latino-americano - a partir do reconhecimento, por parte tanto dos formuladores de política dos Estados Unidos como dos sul-americanos, de que a América do Sul era um sistema separado da América do Norte. Assim, o artigo defende que ao sul do Panamá, a Doutrina Monroe adquiriu um aspecto consideravelmente mais "multilateral", o que foi evidenciado até mesmo pelo seu famoso Corolário Roosevelt.

  6. Foramen of Monro meningioma with atypical appearance: CT and conventional MR findings

    International Nuclear Information System (INIS)

    Hakyemez, B.; Erdogan, C.; Oruc, E.; Parlak, M.; Aker, S.; Aksoy, K.

    2007-01-01

    Full text: Intraventricular meningiomas have often been reported; however, literature reveals very few cases localized within foramen of Monro. Herein we report a 57-year-old woman admitted with obstructive hydrocephalus-related symptoms. Strikingly, the lesion was completely calcified in (T and had no marked solid component on MRI. The lesion was completely removed by surgical resection with a transfrontal intraventricular approach. The resected mass was histopathologically diagnosed as meningioma. The patient's symptoms resolved immediately after the operation

  7. Lawrence Livermore National Laboratory Environmental Report 2015

    International Nuclear Information System (INIS)

    Rosene, C. A.; Jones, H. E.

    2016-01-01

    The purposes of the Lawrence Livermore National Laboratory Environmental Report 2015 are to record Lawrence Livermore National Laboratory's (LLNL's) compliance with environmental standards and requirements, describe LLNL's environmental protection and remediation programs, and present the results of environmental monitoring at the two LLNL sites-the Livermore Site and Site 300. The report is prepared for the U.S. Department of Energy (DOE) by LLNL's Environmental Functional Area. Submittal of the report satisfies requirements under DOE Order 231.1B, ''Environment, Safety and Health Reporting,'' and DOE Order 458.1, ''Radiation Protection of the Public and Environment.''

  8. Endoscopic biopsy of foramen of Monro and third ventricle lesions guided by frameless neuronavigation: usefulness and limitations.

    Science.gov (United States)

    Prat, Ricardo; Galeano, Inmaculada

    2009-09-01

    To describe our institution experience regarding the usefulness and limitations of frameless neuronavigation in the endoscopic biopsy of foramen of Monro and third ventricle lesions. We report our experience with 22 patients harbouring intraventricular lesions located in the region of the foramen of Monro or the third ventricle who underwent endoscopic biopsy guided by the neuronavigation system. Nine lesions were located on the posterior aspect of the third ventricle or at the pineal region, and thirteen lesions were located at the foramen of Monro or anterior third ventricle region. The endoscopes were introduced via an operating sheath, which had previously been inserted with a trocar under neuronavigational control. After approaching the foramen of Monro from the planned angle, surgery was continued under direct visualisation until the lesion was reached, if it was located on the third ventricle. In cases where the lesion was located at the foramen of Monro, an excellent view of the lesion was obtained and neuronavigation was used to determine the location of critical areas. Histological examination of biopsy specimens obtained endoscopically was diagnostic in all cases. Open surgery following endoscopic biopsy was only needed in 1 patient out of 22. In our experience, image-guided neuroendoscopy can improve the accuracy of the endoscopic approach, minimising brain trauma. It can be particularly helpful when performing a brain biopsy in the absence of clear intraventricular landmarks or in the event of adverse visual conditions such as intraventricular bleeding.

  9. Mobile Motion Capture--MiMiC.

    Science.gov (United States)

    Harbert, Simeon D; Jaiswal, Tushar; Harley, Linda R; Vaughn, Tyler W; Baranak, Andrew S

    2013-01-01

    The low cost, simple, robust, mobile, and easy to use Mobile Motion Capture (MiMiC) system is presented and the constraints which guided the design of MiMiC are discussed. The MiMiC Android application allows motion data to be captured from kinematic modules such as Shimmer 2r sensors over Bluetooth. MiMiC is cost effective and can be used for an entire day in a person's daily routine without being intrusive. MiMiC is a flexible motion capture system which can be used for many applications including fall detection, detection of fatigue in industry workers, and analysis of individuals' work patterns in various environments.

  10. A test of Monroe's Motivated Sequence for its effects on ratings of message organization and attitude change.

    Science.gov (United States)

    Micciche, T; Pryor, B; Butler, J

    2000-06-01

    The experiment examined Monroe's Motivated Sequence, an organizational pattern commonly taught in basic speech courses for its effects on attitude change and ratings of comprehensibility of messages. Treatment groups of 21, 23, and 24 participants read one of three versions of a persuasive message that advocated a $50 fee increase to improve parking facilities on campus. One version represented Monroe's five-step sequence, a second version reversed the five steps, and a third randomly ordered the steps. A control group of 22 read a message unrelated to the parking issue. Analysis showed no differences in attitude change across the four groups. The Monroe sequence condition did produce significantly higher ratings of comprehensibility on one of four comparisons.

  11. 78 FR 56609 - Drawbridge Operation Regulations; Reynolds Channel, Lawrence, NY

    Science.gov (United States)

    2013-09-13

    ... Regulations; Reynolds Channel, Lawrence, NY AGENCY: Coast Guard, DHS. ACTION: Notice canceling temporary... Beach Bridge, mile 0.4, across Reynolds Channel, at Lawrence, New York. The owner of the bridge, Nassau... published a temporary deviation entitled ``Drawbridge Operation Regulations; Reynolds Channel, Lawrence, NY...

  12. Lawrence Livermore National Laboratory Environmental Report 2014

    Energy Technology Data Exchange (ETDEWEB)

    Jones, H. E. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Bertoldo, N. A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Blake, R. G. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Buscheck, W. M. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Byrne, J. G. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Cerruti, S. J. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Bish, C. B. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Fratanduono, M. E. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Grayson, A. R. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); MacQueen, D. H. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Montemayor, W. E. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Ottaway, H. L. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Paterson, L. E. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Revelli, M. A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Rosene, C. A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Swanson, K. A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Terrill, A. A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Wegrecki, A. M. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Wilson, K. R. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Woollett, J. S. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2015-09-29

    The purposes of the Lawrence Livermore National Laboratory Environmental Report 2014 are to record Lawrence Livermore National Laboratory’s (LLNL’s) compliance with environmental standards and requirements, describe LLNL’s environmental protection and remediation programs, and present the results of environmental monitoring at the two LLNL sites—the Livermore Site and Site 300. The report is prepared for the U.S. Department of Energy (DOE) by LLNL’s Environmental Functional Area. Submittal of the report satisfies requirements under DOE Order 231.1B, “Environment, Safety and Health Reporting,” and DOE Order 458.1, “Radiation Protection of the Public and Environment.”

  13. Marine geology of the St. Lawrence Estuary

    Energy Technology Data Exchange (ETDEWEB)

    St-Onge, Guillaume [Canada Research Chair in Marine Geology, Institut des sciences de la mer de Rimouski (ISMER) and GEOTOP Research Center, 310 allee des Ursulines, Rimouski, Quebec, G5L 3A1 (Canada); Duchesne, Mathieu J [Geological Survey of Canada, Quebec Division, 490 de la Couronne, Quebec City, Quebec, G1K 9A9 (Canada); Lajeunesse, Patrick, E-mail: guillaume_st-onge@uqar.qc.ca [Departement de geographie and Centre d' etudes nordiques, Universite Laval, Quebec City, Quebec, G1V 0A6 (Canada)

    2011-05-15

    The St. Lawrence Estuary, Eastern Canada, contains a very thick (>450 m) Quaternary sedimentary sequence. The results from recently conducted geophysical surveys in conjunction with piston coring indicate that these sediments were deposited under very high sedimentation rates, sometimes as high as {approx}30 m/ka during the last deglaciation. Results also reveal evidence of large submarine landslides during the Holocene, changes in sedimentation rates and the significant role of submarine canyons and channels to transfer sediments from the coast to the deeper marine environment. Finally, this paper highlights the presence of more than 1900 pockmarks on the seafloor of the St. Lawrence Estuary and discusses their possible origins: active hydrocarbon seeps in the Laurentian Channel and biogenic gas seepage on the northwestern shoulder of the Laurentian Channel.

  14. Lawrence Livermore National Laboratory Environmental Report 2015

    Energy Technology Data Exchange (ETDEWEB)

    Rosene, C. A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Jones, H. E. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2016-09-22

    The purposes of the Lawrence Livermore National Laboratory Environmental Report 2015 are to record Lawrence Livermore National Laboratory’s (LLNL’s) compliance with environmental standards and requirements, describe LLNL’s environmental protection and remediation programs, and present the results of environmental monitoring at the two LLNL sites—the Livermore Site and Site 300. The report is prepared for the U.S. Department of Energy (DOE) by LLNL’s Environmental Functional Area. Submittal of the report satisfies requirements under DOE Order 231.1B, “Environment, Safety and Health Reporting,” and DOE Order 458.1, “Radiation Protection of the Public and Environment.”

  15. Marine geology of the St. Lawrence Estuary

    International Nuclear Information System (INIS)

    St-Onge, Guillaume; Duchesne, Mathieu J; Lajeunesse, Patrick

    2011-01-01

    The St. Lawrence Estuary, Eastern Canada, contains a very thick (>450 m) Quaternary sedimentary sequence. The results from recently conducted geophysical surveys in conjunction with piston coring indicate that these sediments were deposited under very high sedimentation rates, sometimes as high as ∼30 m/ka during the last deglaciation. Results also reveal evidence of large submarine landslides during the Holocene, changes in sedimentation rates and the significant role of submarine canyons and channels to transfer sediments from the coast to the deeper marine environment. Finally, this paper highlights the presence of more than 1900 pockmarks on the seafloor of the St. Lawrence Estuary and discusses their possible origins: active hydrocarbon seeps in the Laurentian Channel and biogenic gas seepage on the northwestern shoulder of the Laurentian Channel.

  16. Lawrence Berkeley Laboratory 1993 Site Environmental Report

    Energy Technology Data Exchange (ETDEWEB)

    1994-05-01

    This annual Site Environmental Report summarizes Lawrence Berkeley Laboratory`s (LBL`s) environmental activities in calendar year (CY) 1993. The purpose of this report is to characterize site environmental management performance, confirm compliance status with environmental standards and requirements, and highlight significant programs and efforts. Its format and content are consistent with the requirements of the US Department of Energy (DOE) Order 5400.1, General Environmental Protection Program.

  17. Environmental report 1997, Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    Lentzner, H.L.; Morris, J.C.; Harrach, R.J.

    1998-01-01

    This report summarizes the environmental program activities at the Lawrence Livermore National Laboratory (LLNL) for 1997. This report accurately summarizes the results of environmental monitoring, compliance, impacts assessment, and the restoration program at LLNL. It features individual chapters on monitoring of air, sewage, surface water, ground water, soil and sediment, vegetation and foodstuff, and environmental radiation. It also contains chapters on site overview, environmental program information, radiological dose assessment, and quality assurance

  18. Superconductor development program at Lawrence Livermore Laboratory

    International Nuclear Information System (INIS)

    Cornish, D.N.

    1978-01-01

    Winding of a Nb--Ti test coil at the Lawrence Livermore Laboratory is nearly complete. The conductor in this coil operates in a maximum field of 7.5 T and provides the 2-T field required by the Mirror Fusion Test Facility. Nb 3 Sn multifilamentary conductors, made using the ''bronze'' technique, appear capable of providing the higher fields needed by commercial reactors

  19. Radiographic testing at Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    Bossi, R.H.

    1982-01-01

    Radiographic testing is a nondestructive inspection technique which uses penetrating radiation. The Nondestructive Evaluation (NDE) Section at Lawrence Livermore National Laboratory has a broad spectrum of equipment and techniques for radiographic testing. These resources include low-energy vacuum systems, low- and mid-energy cabinet and cell radiographic systems, high-energy linear accelerators, portable x-ray machines and radioisotopes for radiographic inspections. For diagnostic testing the NDE Section also has real-time and flash radiographic equipment

  20. Environmental report 1996, Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    Lentzner, H.L.; Napolitano, M.M.; Harrach, R.J.

    1997-01-01

    This report summarizes the environmental program activities at the Lawrence Livermore National Laboratory (LLNL) for 1996. This report accurately summarizes the results of environmental monitoring, compliance, impacts assessment, and the restoration program at LLNL. It features individual chapters on monitoring of air, sewage, surface water, ground water, soil and sediment, vegetation and foodstuff, and environmental radiation. It also contains chapters on site overview, environmental program information, radiological dose assessment, and quality assurance

  1. Life sciences: Lawrence Berkeley Laboratory, 1988

    Energy Technology Data Exchange (ETDEWEB)

    1989-07-01

    Life Sciences Research at LBL has both a long history and a new visibility. The physics technologies pioneered in the days of Ernest O. Lawrence found almost immediate application in the medical research conducted by Ernest's brother, John Lawrence. And the tradition of nuclear medicine continues today, largely uninterrupted for more than 50 years. Until recently, though, life sciences research has been a secondary force at the Lawrence Berkeley Laboratory (LBL). Today, a true multi-program laboratory has emerged, in which the life sciences participate as a full partner. The LBL Human Genome Center is a contribution to the growing international effort to map the human genome. Its achievements represent LBL divisions, including Engineering, Materials and Chemical Sciences, and Information and Computing Sciences, along with Cell and Molecular Biology and Chemical Biodynamics. The Advanced Light Source Life Sciences Center will comprise not only beamlines and experimental end stations, but also supporting laboratories and office space for scientists from across the US. This effort reflects a confluence of scientific disciplines --- this time represented by individuals from the life sciences divisions and by engineers and physicists associated with the Advanced Light Source project. And finally, this report itself, the first summarizing the efforts of all four life sciences divisions, suggests a new spirit of cooperation. 30 figs.

  2. Life sciences: Lawrence Berkeley Laboratory, 1988

    International Nuclear Information System (INIS)

    1989-07-01

    Life Sciences Research at LBL has both a long history and a new visibility. The physics technologies pioneered in the days of Ernest O. Lawrence found almost immediate application in the medical research conducted by Ernest's brother, John Lawrence. And the tradition of nuclear medicine continues today, largely uninterrupted for more than 50 years. Until recently, though, life sciences research has been a secondary force at the Lawrence Berkeley Laboratory (LBL). Today, a true multi-program laboratory has emerged, in which the life sciences participate as a full partner. The LBL Human Genome Center is a contribution to the growing international effort to map the human genome. Its achievements represent LBL divisions, including Engineering, Materials and Chemical Sciences, and Information and Computing Sciences, along with Cell and Molecular Biology and Chemical Biodynamics. The Advanced Light Source Life Sciences Center will comprise not only beamlines and experimental end stations, but also supporting laboratories and office space for scientists from across the US. This effort reflects a confluence of scientific disciplines --- this time represented by individuals from the life sciences divisions and by engineers and physicists associated with the Advanced Light Source project. And finally, this report itself, the first summarizing the efforts of all four life sciences divisions, suggests a new spirit of cooperation. 30 figs

  3. miRSeqNovel

    DEFF Research Database (Denmark)

    Qian, Kui; Auvinen, Eeva; Greco, Dario

    2012-01-01

    We present miRSeqNovel, an R based workflow for miRNA sequencing data analysis. miRSeqNovel can process both colorspace (SOLiD) and basespace (Illumina/Solexa) data by different mapping algorithms. It finds differentially expressed miRNAs and gives conservative prediction of novel miRNA candidates...... with customized parameters. miRSeqNovel is freely available at http://sourceforge.net/projects/mirseq/files....

  4. Obituary: Lawrence Hugh Aller, 1913-2003

    Science.gov (United States)

    Kaler, James B.

    2003-12-01

    The announcement still lies in my inbox: ``Lawrence Aller died last Sunday." On 16 March 2003, one of the world's fine astronomers passed away at the age of 89, leaving behind a legacy that will ripple as long as there are students of the celestial science, one that incorporated observation, theory, education, care, decency, and kindness. Lawrence was born in the humblest of conditions in Tacoma, Washington, on 24 September 1913. His mother, Lella (Belle) Allen, was a homemaker and his father Leslie Aller, was an occassional printer and gold prospector who thought that the use of the mind was a waste of time. With fierce persistence and dedication, Lawrence pulled off a feat that would probably not be possible now: getting into college without having finished high school, the result of being dragged to work in his father's primitive gold mining camp. His interest, sparked by leaflets from the Astronomical Society of the Pacific and by Russell, Dugan, and Stewart's venerable textbook, led him to a correspondence, and finally a meeting, with Donald Menzel of Harvard, who persuaded the admissions director of the University of California at Berkeley to admit him in 1932. From there, Lawrence went on to graduate school at Harvard and the Harvard Society of Fellows, where he studied with Menzel and developed his interest in stellar and nebular astronomy. After working in the War effort, he made his professorial debut at Indiana University, where he stayed until 1948 before leaving for the University of Michigan. Residing there for the next 14 years, he established his research reputation and helped develop the Michigan graduate program. In 1962, the opportunity arose to return to California, to UCLA, where he again was instrumental in founding a PhD program. There he stayed, through his retirement in 1984, doing research right up to the end. Eight other schools received him as visiting professor. Lawrence knew that to make inroads into astronomy, he needed to apply

  5. Lawrence Berkeley Laboratory 1994 site environmental report

    International Nuclear Information System (INIS)

    1995-05-01

    The 1994 Site Environmental Report summarizes environmental activities at Lawrence Berkeley Laboratory (LBL) for the calendar year (CY) 1994. The report strives to present environmental data in a manner that characterizes the performance and compliance status of the Laboratory's environmental management programs when measured against regulatory standards and DOE requirements. The report also discusses significant highlight and planning efforts of these programs. The format and content of the report are consistent with the requirements of the U.S. Department of Energy (DOE) Order 5400.1, General Environmental Protection Program

  6. Lawrence Berkeley Laboratory 1994 site environmental report

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-05-01

    The 1994 Site Environmental Report summarizes environmental activities at Lawrence Berkeley Laboratory (LBL) for the calendar year (CY) 1994. The report strives to present environmental data in a manner that characterizes the performance and compliance status of the Laboratory`s environmental management programs when measured against regulatory standards and DOE requirements. The report also discusses significant highlight and planning efforts of these programs. The format and content of the report are consistent with the requirements of the U.S. Department of Energy (DOE) Order 5400.1, General Environmental Protection Program.

  7. Risk management at Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    Cummings, G.E.; Strait, R.S.

    1993-10-01

    Managing risks at a large national laboratory presents a unique set of challenges. These challenges include the management of a broad diversity of activities, the need to balance research flexibility against management control, and a plethora of requirements flowing from regulatory and oversight bodies. This paper will present the experiences of Lawrence Livermore National Laboratory (LLNL) in risk management and in dealing with these challenges. While general risk management has been practiced successfully by all levels of Laboratory management, this paper will focus on the Laboratory's use of probabilistic safety assessment and prioritization techniques and the integration of these techniques into Laboratory operations

  8. Hydrology, water quality, and effects of drought in Monroe County, Michigan

    Science.gov (United States)

    Nicholas, J.R.; Rowe, Gary L.; Brannen, J.R.

    1996-01-01

    Monroe County relies heavily on its aquifers and streams for drinking water, irrigation, and other ~ses; however, increased water use, high concentrations of certain constituents in ground water, and droughts may limit the availability of water resources. Although the most densely populated parts of the county use water from the Great Lakes, large amounts of ground water are withdrawn for quarry dewatering, domestic supply, and irrigation.Unconsolidated deposits and bedrock of Silurian and Devonian age underlie Mon_roe County. The unconsolidated deposits are mostly clayey and less than 50 feet thick. Usable amounts of ground water generally are obtained from thin, discontinuous surficial sand deposits or, in the northwestern part of the county, from deep glaciofluvial deposits. In most of the county, however, ground water in unconsolidated deposits is highly susceptible to effects of droughts and to contamination.The bedrock is mostly carbonate rock, and usable quantities of ground water can be obtained from fractures and other secondary openings throughout the county. Transmissivities of the Silurian-Devonian aquifer range from 10 to 6,600 feet squared per day. Aquifer tests and historical informati.on indicate that the Silurian-Devonian aquifer is confmed throughout most of the county. The major recharge area for the Silurian-Devonian aquifer in Monroe County is in the southwest, and groundwater flow is mostly southeastward toward Lake Erie. In the northeastern and southeastern parts of the county, the potentiometric surface of the SilurianDevonian aquifers has been lowered by pumpage to below the elevation of Lake Erie.Streams and artificial drains in Monroe County are tributary to Lake Erie. Most streams are perennial because of sustained discharge from the sand aquifer and the Silurian-Devonian aquifer; however, the lower reaches of River Raisin and Plum Creek lost water to the Silurian-Devonian aquifer in July 1990.The quality of ground water and of

  9. Euroopal tuleb leida vastus Vene Monroe-doktriinile / Lennart Meri ; intervjueerinud Olaf Ihlan, Andreas Lorentz

    Index Scriptorium Estoniae

    Meri, Lennart, 1929-2006

    1993-01-01

    Eesti Vabariigi president L. Meri Baltikumist ja suurvene šovinismist. Intervjuu ajakirjale "Der Spiegel", nr. 44, 1. nov. 1993, lk. 200, 202-203, 206, pealkiri: "Dann wird Europa verbluten" : Estlands Präsident Lennart Meri über das Baltikum und den grossrussischen Chauvinismus. Ilmunud ka: Õhtuleht, 8. nov., lk. 5, pealkiri: "Siis voolab Euroopas veri" : Eesti president Lennart Meri Baltikumist ja suurvene šovinismist ; Rahva Hääl, 9. nov., lk., 3 ; Eesti Rada (Köln, 1993) nr. 6, lk. 3, pealkiri: President Lennart Meri intervjuu "Spiegelile" / refereerinud ja tsiteerinud J. P ; Eesti Päevaleht (Stockholm), 24. nov., lk. 2 : ill., pealkiri: President Lennart Meri : "Venemaa laiendab oma huvisfääri" ; Vaba Eestlane (Toronto), 9. dets., lk. 3, 11, pealkiri: "Euroopal tuleb leida vastus Vene - Monroe doktriinile" ; Meie Kodu, 15. dets., lk. 6, pealkiri: Eesti Vabariigi President Lennart Meri Baltikumist ja suurvene šovinismist

  10. Autogrammid, oma aja märk / Mike Lawrence

    Index Scriptorium Estoniae

    Lawrence, Mike

    2004-01-01

    Autogrammide kogumisest, nende ehtsusest, sportlastele kuulunud esemete kollektsioneerimisest. Lisatud: Kollektsionääride maiuspalu. Autor Mike Lawrence on oksjonifirma Bonhams/Brooks konsultant, ajakirjanik

  11. Pharmaco-miR

    DEFF Research Database (Denmark)

    Rukov, Jakob Lewin; Wilentzik, Roni; Jaffe, Ishai

    2014-01-01

    MicroRNAs (miRNAs) are short regulatory RNAs that down-regulate gene expression. They are essential for cell homeostasis and active in many disease states. A major discovery is the ability of miRNAs to determine the efficacy of drugs, which has given rise to the field of 'miRNA pharmacogenomics......' through 'Pharmaco-miRs'. miRNAs play a significant role in pharmacogenomics by down-regulating genes that are important for drug function. These interactions can be described as triplet sets consisting of a miRNA, a target gene and a drug associated with the gene. We have developed a web server which...... links miRNA expression and drug function by combining data on miRNA targeting and protein-drug interactions. miRNA targeting information derive from both experimental data and computational predictions, and protein-drug interactions are annotated by the Pharmacogenomics Knowledge base (Pharm...

  12. Geologic map of Saint Lawrence Island, Alaska

    Science.gov (United States)

    Patton, William W.; Wilson, Frederic H.; Taylor, Theresa A.

    2011-01-01

    Saint Lawrence Island is located in the northern Bering Sea, 190 km southwest of the tip of the Seward Peninsula, Alaska, and 75 km southeast of the Chukotsk Peninsula, Russia (see index map, map sheet). It lies on a broad, shallow-water continental shelf that extends from western Alaska to northeastern Russia. The island is situated on a northwest-trending structural uplift exposing rocks as old as Paleozoic above sea level. The submerged shelf between the Seward Peninsula and Saint Lawrence Island is covered mainly with Cenozoic deposits (Dundo and Egiazarov, 1982). Northeast of the island, the shelf is underlain by a large structural depression, the Norton Basin, which contains as much as 6.5 km of Cenozoic strata (Grim and McManus, 1970; Fisher and others, 1982). Sparse test-well data indicate that the Cenozoic strata are underlain by Paleozoic and Proterozoic rocks, similar to those exposed on the Seward Peninsula (Turner and others, 1983). Saint Lawrence Island is 160 km long in an east-west direction and from 15 km to 55 km wide in a north-south direction. The east end of the island consists largely of a wave-cut platform, which has been elevated as much as 30 m above sea level. Isolated upland areas composed largely of granitic plutons rise as much as 550 m above the wave-cut platform. The central part of the island is dominated by the Kookooligit Mountains, a large Quaternary shield volcano that extends over an area of 850 km2 and rises to an elevation of 630 m. The west end of the island is composed of the Poovoot Range, a group of barren, rubble-covered hills as high as 450 m that extend from Boxer Bay on the southwest coast to Taphook Mountain on the north coast. The Poovoot Range is flanked on the southeast by the Putgut Plateau, a nearly flat, lake-dotted plain that stands 30?60 m above sea level. The west end of the island is marked by uplands underlain by the Sevuokuk pluton (unit Kg), a long narrow granite body that extends from Gambell on the

  13. Lawrence Berkeley Laboratory Affirmative Action Program. Revised

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-06-01

    The Lawrence Berkeley Laboratory`s Affirmative Action Program (AAP) serves as a working document that describes current policies, practices, and results in the area of affirmative action. It represents the Laboratory`s framework for an affirmative approach to increasing the representation of people of color and women in segments of our work force where they have been underrepresented and taking action to increase the employment of persons with disabilities and special disabled and Vietnam era veterans. The AAP describes the hierarchy of responsibility for Laboratory affirmative action, the mechanisms that exist for full Laboratory participation in the AAP, the policies and procedures governing recruitment at all levels, the Laboratory`s plan for monitoring, reporting, and evaluating affirmative action progress, and a description of special affirmative action programs and plans the Laboratory has used and will use in its efforts to increase the representation and retention of groups historically underrepresented in our work force.

  14. 78 FR 66265 - Drawbridge Operation Regulations; Reynolds Channel, Lawrence, NY

    Science.gov (United States)

    2013-11-05

    ... Regulations; Reynolds Channel, Lawrence, NY AGENCY: Coast Guard, DHS. ACTION: Notice of temporary deviation... from the regulations governing the operation of the Atlantic Beach Bridge, mile 0.4, across Reynolds.... SUPPLEMENTARY INFORMATION: The Atlantic Beach Bridge, across Reynolds Channel, mile 0.4, at Lawrence, New York...

  15. 78 FR 34893 - Drawbridge Operation Regulations; Reynolds Channel, Lawrence, NY

    Science.gov (United States)

    2013-06-11

    ... Regulations; Reynolds Channel, Lawrence, NY AGENCY: Coast Guard, DHS. ACTION: Notice of temporary deviation... from the regulations governing the operation of the Atlantic Beach Bridge, mile 0.4, across Reynolds... Reynolds Channel, mile 0.4, at Lawrence, New York, has a vertical clearance in the closed position of 25...

  16. 78 FR 56610 - Drawbridge Operation Regulations; Reynolds Channel, Lawrence, NY

    Science.gov (United States)

    2013-09-13

    ... Regulations; Reynolds Channel, Lawrence, NY AGENCY: Coast Guard, DHS. ACTION: Notice of temporary deviation... from the regulations governing the operation of the Atlantic Beach Bridge, mile 0.4, across Reynolds.... SUPPLEMENTARY INFORMATION: The Atlantic Beach Bridge, across Reynolds Channel, mile 0.4, at Lawrence, New York...

  17. Direct utilization of geothermal resources field experiments at Monroe, Utah. Final report, July 14, 1978-July 13, 1981

    Energy Technology Data Exchange (ETDEWEB)

    Blair, C.K.; Owen, L.B. (eds.)

    1982-12-01

    The City of Monroe, Utah undertook a project to demonstrate the economic and technical viability of utilizing a low temperature geothermal resource to provide space and hot water heating to commercial, municipal, and domestic users within the community. During the course of the project, resource development and assessment, including drilling of a production well, was successfully completed. Upon completion of the field development and assessment phase of the program and of a preliminary design of the district heating system, it was determined that the project as proposed was not economically viable. This was due to: (1) a significant increase in estimated capital equipment costs resulting from the general inflation in construction costs, the large area/low population density in Monroe, and a more remote fluid disposal well site than planned, could not balance increased construction costs, (2) a lower temperature resource than predicted, and (3) due to predicted higher pumping and operating costs. After a thorough investigation of alternatives for utilizing the resource, further project activities were cancelled because the project was no longer economical and an alternative application for the resource could not be found within the constraints of the project. The City of Monroe, Utah is still seeking a beneficial use for the 600 gpm, 164/sup 0/F geothermal well. A summary of project activities included.

  18. Lawrence Livermore National Laboratory Environmental Report 2010

    Energy Technology Data Exchange (ETDEWEB)

    Jones, H E; Bertoldo, N A; Campbell, C G; Cerruti, S J; Coty, J D; Dibley, V R; Doman, J L; Grayson, A R; MacQueen, D H; Wegrecki, A M; Armstrong, D H; Brigdon, S L; Heidecker, K R; Hollister, R K; Khan, H N; Lee, G S; Nelson, J C; Paterson, L E; Salvo, V J; Schwartz, W W; Terusaki, S H; Wilson, K R; Woods, J M; Yimbo, P O; Gallegos, G M; Terrill, A A; Revelli, M A; Rosene, C A; Blake, R G; Woollett, J S; Kumamoto, G

    2011-09-14

    The purposes of the Lawrence Livermore National Laboratory Environmental Report 2010 are to record Lawrence Livermore National Laboratory's (LLNL's) compliance with environmental standards and requirements, describe LLNL's environmental protection and remediation programs, and present the results of environmental monitoring at the two LLNL sites - the Livermore site and Site 300. The report is prepared for the U.S. Department of Energy (DOE) by LLNL's Environmental Protection Department. Submittal of the report satisfies requirements under DOE Order 231.1A, Environmental Safety and Health Reporting, and DOE Order 5400.5, Radiation Protection of the Public and Environment. The report is distributed electronically and is available at https://saer.llnl.gov/, the website for the LLNL annual environmental report. Previous LLNL annual environmental reports beginning in 1994 are also on the website. Some references in the electronic report text are underlined, which indicates that they are clickable links. Clicking on one of these links will open the related document, data workbook, or website that it refers to. The report begins with an executive summary, which provides the purpose of the report and an overview of LLNL's compliance and monitoring results. The first three chapters provide background information: Chapter 1 is an overview of the location, meteorology, and hydrogeology of the two LLNL sites; Chapter 2 is a summary of LLNL's compliance with environmental regulations; and Chapter 3 is a description of LLNL's environmental programs with an emphasis on the Environmental Management System including pollution prevention. The majority of the report covers LLNL's environmental monitoring programs and monitoring data for 2010: effluent and ambient air (Chapter 4); waters, including wastewater, storm water runoff, surface water, rain, and groundwater (Chapter 5); and terrestrial, including soil, sediment, vegetation, foodstuff

  19. Lawrence Livermore National Laboratory Environmental Report 2013

    Energy Technology Data Exchange (ETDEWEB)

    Jones, H. E. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Bertoldo, N. A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Blake, R. G. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Cerruti, S. J. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Dibley, V. R. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Doman, J. L. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Fish, C. B. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Grayson, A. R. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Heidecker, K. R. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Kumamoto, G. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); MacQueen, D. H. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Montemayor, W. E. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Ottaway, H. L. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Paterson, L. E. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Revelli, M. A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Rosene, C. A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Terrill, A. A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Wegrecki, A. M. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Wilson, K. R. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Woollett, J. S. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Veseliza, R. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2014-10-01

    Lawrence Livermore National Laboratory (LLNL) is a premier research laboratory that is part of the National Nuclear Security Administration (NNSA) within the U.S. Department of Energy (DOE). As a national security laboratory, LLNL is responsible for ensuring that the nation’s nuclear weapons remain safe, secure, and reliable. The Laboratory also meets other pressing national security needs, including countering the proliferation of weapons of mass destruction and strengthening homeland security, and conducting major research in atmospheric, earth, and energy sciences; bioscience and biotechnology; and engineering, basic science, and advanced technology. The Laboratory is managed and operated by Lawrence Livermore National Security, LLC (LLNS), and serves as a scientific resource to the U.S. government and a partner to industry and academia. LLNL operations have the potential to release a variety of constituents into the environment via atmospheric, surface water, and groundwater pathways. Some of the constituents, such as particles from diesel engines, are common at many types of facilities while others, such as radionuclides, are unique to research facilities like LLNL. All releases are highly regulated and carefully monitored. LLNL strives to maintain a safe, secure and efficient operational environment for its employees and neighboring communities. Experts in environment, safety and health (ES&H) support all Laboratory activities. LLNL’s radiological control program ensures that radiological exposures and releases are reduced to as low as reasonably achievable to protect the health and safety of its employees, contractors, the public, and the environment. LLNL is committed to enhancing its environmental stewardship and managing the impacts its operations may have on the environment through a formal Environmental Management System. The Laboratory encourages the public to participate in matters related to the Laboratory’s environmental impact on the

  20. Lawrence Livermore National Laboratory Environmental Report 2012

    Energy Technology Data Exchange (ETDEWEB)

    Jones, Henry E. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Armstrong, Dave [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Blake, Rick G. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Bertoldo, Nicholas A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Cerruti, Steven J. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Fish, Craig [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Dibley, Valerie R. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Doman, Jennifer L. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Grayson, Allen R. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Heidecker, Kelly R. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Hollister, Rod K. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Kumamoto, Gene [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); MacQueen, Donald H. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Nelson, Jennifer C. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Ottaway, Heather L. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Paterson, Lisa E. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Revelli, Michael A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Rosene, Crystal A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Terrill, Alison A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Wegrecki, Anthony M. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Wilson, Kent R. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Woollett, Jim S. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2013-09-19

    Lawrence Livermore National Laboratory (LLNL) is a premier research laboratory that is part of the National Nuclear Security Administration (NNSA) within the U.S. Department of Energy (DOE). As a national security laboratory, LLNL is responsible for ensuring that the nation’s nuclear weapons remain safe, secure, and reliable. The Laboratory also meets other pressing national security needs, including countering the proliferation of weapons of mass destruction and strengthening homeland security, and conducting major research in atmospheric, earth, and energy sciences; bioscience and biotechnology; and engineering, basic science, and advanced technology. The Laboratory is managed and operated by Lawrence Livermore National Security, LLC (LLNS), and serves as a scientific resource to the U.S. government and a partner to industry and academia. LLNL operations have the potential to release a variety of constituents into the environment via atmospheric, surface water, and groundwater pathways. Some of the constituents, such as particles from diesel engines, are common at many types of facilities while others, such as radionuclides, are unique to research facilities like LLNL. All releases are highly regulated and carefully monitored. LLNL strives to maintain a safe, secure and efficient operational environment for its employees and neighboring communities. Experts in environment, safety and health (ES&H) support all Laboratory activities. LLNL’s radiological control program ensures that radiological exposures and releases are reduced to as low as reasonably achievable to protect the health and safety of its employees, contractors, the public, and the environment. LLNL is committed to enhancing its environmental stewardship and managing the impacts its operations may have on the environment through a formal Environmental Management System. The Laboratory encourages the public to participate in matters related to the Laboratory’s environmental impact on the

  1. A 2-D simulation of hydrocephalus in the Foramens of Monro of the human ventricular system

    Energy Technology Data Exchange (ETDEWEB)

    Ammourah, S.; Aroussi, A. [Univ. of Nottingham, School of Mechanical, Materials, Manufacturing and Management, University Park, Nottingham (United Kingdom)]. E-mail: eaxsaka@nottingham.ac.uk; Vloeberghs, M. [Queen' s Medical Centre, Dept. of Child Health, Nottingham (United Kingdom)

    2004-07-01

    This study investigates the Cerebrospinal fluid (CSF) flow behavior in a two-dimensional plane (2-D) of the human ventricular system when the hydrocephalus in the Foramens of Monro (F.O.M) occurs. In order to understand the CSF behaviour when the hydrocephalus occurs, it is essential to comprehend its normal flow dynamics i.e. the healthy case with no hydrocephalus. This had been done numerically by reconstructing the ventricular system geometry from the MRI scans and then made a 15{sup o} degree cut in the surgical line to obtain the outlines of the 2-D plane. The healthy cases were solved for the CSF actual flow rate, which is between 100-500 ml/day in 100 incremental steps. The unhealthy cases were studied for the average flow rate, which is 300 ml/day but for blockage ratios of the Foramen diameter of 20%, 50% and 75%. These obstructions are designed to simulate the hydrocephalus. The results show that as the flow rate increases the pressure and the velocity values increases, but no changes in the flow pattern occurs. The maximum pressure on the normal cases occurred in the lateral ventricles and the maximum velocity occurred in the aqueduct neck. The results of the hydrocephalus cases show that as the obstruction degree increases the pressure in the lateral ventricle increases accompanied with a velocity increase in the obstructed Foramen, which may cause unwanted stress on the neighboring tissues. (author)

  2. A 2-D simulation of hydrocephalus in the Foramens of Monro of the human ventricular system

    International Nuclear Information System (INIS)

    Ammourah, S.; Aroussi, A.; Vloeberghs, M.

    2004-01-01

    This study investigates the Cerebrospinal fluid (CSF) flow behavior in a two-dimensional plane (2-D) of the human ventricular system when the hydrocephalus in the Foramens of Monro (F.O.M) occurs. In order to understand the CSF behaviour when the hydrocephalus occurs, it is essential to comprehend its normal flow dynamics i.e. the healthy case with no hydrocephalus. This had been done numerically by reconstructing the ventricular system geometry from the MRI scans and then made a 15 o degree cut in the surgical line to obtain the outlines of the 2-D plane. The healthy cases were solved for the CSF actual flow rate, which is between 100-500 ml/day in 100 incremental steps. The unhealthy cases were studied for the average flow rate, which is 300 ml/day but for blockage ratios of the Foramen diameter of 20%, 50% and 75%. These obstructions are designed to simulate the hydrocephalus. The results show that as the flow rate increases the pressure and the velocity values increases, but no changes in the flow pattern occurs. The maximum pressure on the normal cases occurred in the lateral ventricles and the maximum velocity occurred in the aqueduct neck. The results of the hydrocephalus cases show that as the obstruction degree increases the pressure in the lateral ventricle increases accompanied with a velocity increase in the obstructed Foramen, which may cause unwanted stress on the neighboring tissues. (author)

  3. 2010 ARRA Lidar: 4 Southeast Counties (MI)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — TASK NAME: Southeast Michigan LiDAR LiDAR Data Acquisition and Processing Production Task- Monroe, St. Clair, Macomb, and Livingston Counties SEMCOG CONTRACT:...

  4. Lawrence Livermore National Laboratory 2007 Annual Report

    Energy Technology Data Exchange (ETDEWEB)

    Chrzanowski, P; Walter, K

    2008-04-25

    Lawrence Livermore National Laboratory's many outstanding accomplishments in 2007 are a tribute to a dedicated staff, which is shaping the Laboratory's future as we go through a period of transition and transformation. The achievements highlighted in this annual report illustrate our focus on the important problems that affect our nation's security and global stability, our application of breakthrough science and technology to tackle those problems, and our commitment to safe, secure, and efficient operations. In May 2007, the Department of Energy (DOE) awarded Lawrence Livermore National Security, LLC (LLNS), a new public-private partnership, the contract to manage and operate the Laboratory starting in October. Since its inception in 1952, the Laboratory had been managed by the University of California (UC) for the DOE's National Nuclear Security Administration (NNSA) and predecessor organizations. UC is one of the parent organizations that make up LLNS, and UC's presence in the new management entity will help us carry forward our strong tradition of multidisciplinary science and technology. 'Team science' applied to big problems was pioneered by the Laboratory's co-founder and namesake, Ernest O. Lawrence, and has been our hallmark ever since. Transition began fully a year before DOE's announcement. More than 1,600 activities had to be carried out to transition the Laboratory from management by a not-for-profit to a private entity. People, property, and procedures as well as contracts, formal agreements, and liabilities had to be transferred to LLNS. The pre-transition and transition teams did a superb job, and I thank them for their hard work. Transformation is an ongoing process at Livermore. We continually reinvent ourselves as we seek breakthroughs that impact emerging national needs. An example is our development in the late 1990s of a portable instrument that could rapidly detect DNA signatures, research that

  5. Annual environmental monitoring report of the Lawrence Berkeley Laboratory, 1980

    International Nuclear Information System (INIS)

    Schleimer, G.E.

    1981-04-01

    The Environmental Monitoring Program of the Lawrence Berkeley Laboratory is described. Data on air and water sampling and continuous radiation monitoring for 1980 are presented, and general trends are discussed

  6. D.H. Lawrence's conception of the unconscious | Eruvbetine | Lagos ...

    African Journals Online (AJOL)

    , this work compares his work with that of Freud, as cited, utilised and criticised by Lawrence. Similarities are established in their basic delimitations of the unconscious while differences in their approaches are identified as the main source of ...

  7. EPA Selects Lawrence, Mass. Group for Brownfields Job Training Grant

    Science.gov (United States)

    Today, EPA announced that the Merrimack Valley Workforce Investment Board, of Lawrence, Mass., was one of 14 organizations nationwide selected to receive funding to operate environmental job training programs for local unemployed residents.

  8. Manufacturing of neutral beam sources at Lawrence Livermore Laboratory

    International Nuclear Information System (INIS)

    Baird, E.D.; Duffy, T.J.; Harter, G.A.; Holland, E.D.; Kloos, W.A.; Pastrone, J.A.

    1979-01-01

    Over 50 neutral beam sources (NBS) of the joint Lawrence Berkeley Laboratory (LBL)/Lawrence Livermore Laboratory (LLL) design have been manufactured, since 1973, in the LLL Neutral Beam Source Facility. These sources have been used to provide start-up and sustaining neutral beams for LLL mirror fusion experiments, including 2XIIB, TMX, and Beta II. Experimental prototype 20-kV and 80-kV NBS have also been designed, built, and tested for the Mirror Fusion Test Facility (MFTF)

  9. Bir Ahlak Eğitimcisi Olarak Lawrence Kohlberg

    OpenAIRE

    Çinemre, Arş. Gör. Semra

    2013-01-01

    Lawrence Kohlberg: As A Moral Educator Lawrence Kohlberg (1927-1987) is a scholar who has comprehensive knowledge of many fields especially philosophy, psychology, sociology, anthropology and education, and who is known clearly for cognitive moral development theory. Having looked at the works in national and international scale which are done on Kohlberg, it is seen generally that his moral development theory has been emphasized and that his moral educational works have been neglected. In fa...

  10. Hydrocephalus caused by unilateral foramen of Monro obstruction: A review on terminology

    Science.gov (United States)

    Nigri, Flavio; Gobbi, Gabriel Neffa; da Costa Ferreira Pinto, Pedro Henrique; Simões, Elington Lannes; Caparelli-Daquer, Egas Moniz

    2016-01-01

    Background: Hydrocephalus caused by unilateral foramen of Monro (FM) obstruction has been referred to in literature by many different terminologies. Precise terminology describing hydrocephalus confined to just one lateral ventricle has a very important prognostic value and determines whether or not the patient can be shunt free after an endoscopic procedure. Methods: Aiming to define the best term for unilateral FM obstruction, 19 terms were employed on PubMed database (http://www.ncbi.nlm.nih.gov/pubmed) as quoted phrases. Results: A total of 194 articles were found. Four patterns of hydrocephalus were discriminated as a result of our research term query and were divided by types for didactic purpose. Type A - partial dilation of the lateral ventricle; Type B - pure unilateral obstruction of the FM; Type C - previously shunted patients with secondary obstruction of the FM; and Type D - asymmetric lateral ventricles with patent FM. Conclusion: In unilateral FM obstruction hydrocephalus, an in-depth review on terminology application is critical to avoid mistakes that may compromise comparisons among different series. This terminology review suggests that Type B hydrocephalus, i.e., the hydrocephalus confined to just one lateral ventricle with no other sites of cerebrospinal fluid circulation blockage, are best described by the terms unilateral hydrocephalus (UH) and monoventricular hydrocephalus, the first being by far the most popular. Type A hydrocephalus is best represented in the literature by the terms uniloculated hydrocephalus and loculated ventricle; Type C hydrocephalus by the terms isolated lateral ventricle and isolated UH; and Type D hydrocephalus by the term asymmetric hydrocephalus. PMID:27274402

  11. Obituary: Fred Lawrence Whipple, 1906-2004

    Science.gov (United States)

    Yeomans, Donald Keith

    2004-12-01

    Fred Whipple, one of the founding fathers of planetary science, died on August 30, 2004 just two months shy of his 98th birthday. The breadth of Fred's published research from 1927 through 2000 is quite extraordinary. Although his collected works were published in two massive volumes in 1972, shortly before his retirement, Fred's research contributions continued for another three decades - and another volume is planned. Fred Lawrence Whipple was born on November 5, 1906 on a farm in Red Oak Iowa. His parents were Harry Lawrence and Celestia (MacFarl) Whipple. At the age of fifteen, the Whipple family moved to California where Fred studied mathematics at Occidental College and the University of California at Los Angeles. As a graduate student at the University of California at Berkeley in 1930, he was one of the first to compute an orbit for the newly discovered planet Pluto. Upon receiving his PhD in 1931, he joined the staff of the Harvard College Observatory. He was Chairman of the Harvard Department of Astronomy (1949 - 1956), Director or the Smithsonian Astrophysical Observatory (1955 - 1973), Phillips Professor of Astronomy (1968 - 1977) and Emeritus Phillips Professor of astronomy (1977 - 2004). In 1928 he married Dorothy Woods and their son, Earle Raymond, survives him. The marriage ended in divorce in 1935. Eleven years later, he married Babette F. Samelson and she too survives him, as do their two daughters Laura and (Dorothy) Sandra. Shortly after arriving at Harvard in the early 1930's, Fred developed a photographic tracking network to determine meteor trajectories from simultaneous observations from two or more stations. The photographic trails, chopped by a rotating shutter, allowed their orbits in space to be determined accurately. With the strong involvement of Richard McCrosky and others, he concluded in the early 1960's that most of these meteors were on comet-like orbits and less than 1% of the naked eye, sporadic meteors could be traced to an

  12. O caso Marylin Monroe: evidências da forclusão do sujeito e de seu ato Marylin Monroe's case: evidences of the subject's Verwerfung and of its act

    Directory of Open Access Journals (Sweden)

    Jéferson Machado Pinto

    2012-06-01

    Full Text Available A objetivação da transferência sustenta uma posição epistemológica característica do discurso da ciência e implica a forclusão do real do sujeito e de sua verdade, excluindo a possibilidade do ato analítico. Para mostrá-lo, o trabalho utiliza dados biográficos e relatos das últimas sessões da análise de Marylin Monroe. Destaca que o masoquismo, vertente real do enlaçamento amoroso da atriz com o Outro, foi elidido em favor da interpretação simbólica do inconsciente e da transferência tomada como simples repetição das relações de objeto, com trágicas consequências para o sujeito.The transfer objectification is based on an epistemological position characteristic of the science discourse and implies the Verwerfung of the subject's real and of its truth, excluding the possibility of the analytical act. To discuss it, this paper uses biographical data and reports of Marylin Monroe's last analytical sessions emphasizing that masochism, the real part of the actress' love engagements with the Other, was ignored in favor of the symbolic interpretation of the unconscious and of the transfer taken as a mere repetition of the object relationships, with tragic consequences on the subject.

  13. Ernest Orlando Lawrence (1901-1958), Cyclotron and Medicine

    Energy Technology Data Exchange (ETDEWEB)

    Chu, William T.

    2005-09-01

    On August 8, 2001, Lawrence Berkeley National Laboratory celebrated the centennial of the birth of its founder (and namesake), Ernest Orlando Lawrence. For the occasion, many speeches were given and old speeches were remembered. We recall the words of the late Luis Alvarez, a Nobel Laureate and one of the Lawrence's closest colleagues: ''Lawrence will always be remembered as the inventor of the cyclotron, but more importantly, he should be remembered as the inventor of the modern way of doing science''. J. L. Heilbron and R. W. Seidel, in the introduction of their book, ''Lawrence and His Laboratory'' stated, ''The motives and mechanisms that shaped the growth of the Laboratory helped to force deep changes in the scientific estate and in the wider society. In the entrepreneurship of its founder, Ernest Orlando Lawrence, these motives, mechanisms, and changes came together in a tight focus. He mobilized great and small philanthropists, state and local governments, corporations, and plutocrats, volunteers and virtuosos. The work they supported, from astrophysics and atomic bombs, from radiochemistry to nuclear medicine, shaped the way we observe, control, and manipulate our environment.'' Indeed, all over the civilized world, the ways we do science changed forever after Lawrence built his famed Radiation Laboratory. In this editorial, we epitomize his legacy of changing the way we do medicine, thereby affecting the health and well being of all humanity. This year marks the 75th anniversary of the invention of the cyclotron by Ernest Orlando Lawrence at the University of California at Berkeley. Lawrence conceived the idea of the cyclotron early in 1929 after reading an article by Rolf Wideroe on high-energy accelerators. In the spring of 1930 one of his students, Nels Edlefsen, constructed two crude models of a cyclotron. Later in the fall of the same year, another student, M. Stanley Livingston

  14. Annual environmental monitoring report of the Lawrence Berkeley Laboratory, 1986

    International Nuclear Information System (INIS)

    Schleimer, G.E.

    1987-04-01

    The Environmental Monitoring Program of the Lawrence Berkeley Laboratory is described. Data for 1986 are presented and general trends are discussed. Topics include radiation monitoring, wastewater discharge monitoring, dose distribution estimates, and ground water monitoring. 9 refs., 8 figs., 20 tabs

  15. Accelerator safety program at the Lawrence Livermore Laboratory

    International Nuclear Information System (INIS)

    Graham, C.L.

    1976-01-01

    A proposed accelerator safety standard for the Lawrence Livermore Laboratory (LLL) is given. All accelerators will comply with this standard when it is included in the LLL Health and Safety Manual. The radiation alarm and radiation safety system for a radiography facility are also described

  16. Crystal clear the autobiographies of Sir Lawrence and Lady Bragg

    CERN Document Server

    Thomson, Patience

    2015-01-01

    The main body of this book contains the hitherto unpublished autobiographies of both William Lawrence Bragg, an innovative scientist who won the Nobel Prize for Physics in 1915, and his wife, Alice, a Mayor of Cambridge and National Chairman of Marriage Guidance. Their autobiographies give unusual insights into the lives and times of two distinguished people and the real personalities behind their public appearance.

  17. Environmental monitoring at the Lawrence Livermore Laboratory. 1979 annual report

    International Nuclear Information System (INIS)

    Silver, W.J.; Lindeken, C.L.; White, J.H.; Buddemeir, R.W.

    1980-01-01

    Information on monitoring activities is reported in two sections for EDB/ERA/INIS. The first section covers all information reported except Appendix D, which gives details of sampling and analytical procedures for environmental monitoring used at Lawrence Livermore Laboratory. A separate abstract was prepared for Appendix D

  18. Waste management study: Process development at Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    1984-12-01

    This report presents the results of an evaluation of the present Toxic Waste Control Operations at the Lawrence Livermore National Laboratory, evaluates the technologies most applicable to the treatment of toxic and hazardous wastes and presents conceptual designs of processes for the installation of a new decontamination and waste treatment facility (DWTF) for future treatment of these wastes

  19. Astronaut Wendy Lawrence participates in training session in the CCT

    Science.gov (United States)

    1994-01-01

    Seated in the pilot's seat of a JSC Shuttle trainer, astronaut Wendy B. Lawrence, STS-67 flight engineer, participates in a training session. The 1992 astronaut class graduate is in the crew compartment trainer (CCT) of JSC's Shuttle mockup and integration laboratory.

  20. Lawrence Berkeley Laboratory Institutional Plan FY 1995--2000

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1994-12-01

    This report presents the details of the mission and strategic plan for Lawrence Berkeley Laboratory during the fiscal years of 1995--2000. It presents summaries of current programs and potential changes; critical success factors such as human resources; management practices; budgetary allowances; and technical and administrative initiatives.

  1. High energy laser facilities at Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    Holmes, N.C.

    1981-06-01

    High energy laser facilities at Lawrence Livermore National Laboratory are described, with special emphasis on their use for equation of state investigations using laser-generated shockwaves. Shock wave diagnostics now in use are described. Future Laboratory facilities are also discussed

  2. O mito da serpente em D. H. Lawrence = The serpent myth in D. H. Lawrence

    Directory of Open Access Journals (Sweden)

    Ana Maria Leal Cardoso

    2008-01-01

    Full Text Available Este trabalho propõe um estudo do mito da serpente, na obra A serpenteemplumada, de D. H. Lawrence, baseado na trajetória mítica do herói, estabelecida por Joseph Campbell e na crítica junguiana da cultura. Kate, a protagonista, empreende uma busca de renascimento psicológico e espiritual, ao chegar à terra primitiva do México. Tal busca simboliza um retorno ao mundo primitivo da Grande Mãe, entendido, do ponto de vista psicológico, como o inconsciente. Assim como o processo de individuação, descrito por Jung, essa personagem enfrenta rompimentos e dificuldades no decorrer da expansão da consciência. Neste sentido, a serpente simboliza tanto o que é viperino na natureza humana, quanto à sabedoria do corpo e dos instintos.This paper proposes a study on the myth of the serpent in D. H. Lawrence’s The Plumed Serpent, based on Joseph Campbell’s mythical map of the hero’s journey and Jungian criticism. Kate, the femaleprotagonist, embarks on a quest for spiritual-psychological rebirth in the primitive lands of Mexico. Following the pattern of Jung’s individuation process and exploring many recurrent symbols in the narrative, we intend to show how Kate faces ruptures anduncertainties as her consciousness is getting into a process of expansion. According to our analysis, the serpent illustrates the Self’s paradoxical aspect, representing both the viper sideof the human being as well as its knowledge.

  3. Search for <mi>CP> Violation and Measurement of the Branching Fraction in the Decay <mi>D>0<mi>KS>0<mi>KS>0

    Energy Technology Data Exchange (ETDEWEB)

    Dash, N.; Bahinipati, S.; Bhardwaj, V.; Trabelsi, K.; Adachi, I.; Aihara, H.; Al Said, S.; Asner, D. M.; Aulchenko, V.; Aushev, T.; Ayad, R.; Babu, V.; Badhrees, I.; Bakich, A. M.; Bansal, V.; Barberio, E.; Bhuyan, B.; Biswal, J.; Bobrov, A.; Bondar, A.; Bonvicini, G.; Bozek, A.; Bračko, M.; Breibeck, F.; Browder, T. E.; Červenkov, D.; Chang, M. -C.; Chekelian, V.; Chen, A.; Cheon, B. G.; Chilikin, K.; Cho, K.; Choi, Y.; Cinabro, D.; Di Carlo, S.; Doležal, Z.; Drásal, Z.; Dutta, D.; Eidelman, S.; Epifanov, D.; Farhat, H.; Fast, J. E.; Ferber, T.; Fulsom, B. G.; Gaur, V.; Gabyshev, N.; Garmash, A.; Gillard, R.; Goldenzweig, P.; Haba, J.; Hara, T.; Hayasaka, K.; Hayashii, H.; Hedges, M. T.; Hou, W. -S.; Iijima, T.; Inami, K.; Ishikawa, A.; Itoh, R.; Iwasaki, Y.; Jacobs, W. W.; Jaegle, I.; Jeon, H. B.; Jin, Y.; Joffe, D.; Joo, K. K.; Julius, T.; Kahn, J.; Kaliyar, A. B.; Karyan, G.; Katrenko, P.; Kawasaki, T.; Kiesling, C.; Kim, D. Y.; Kim, H. J.; Kim, J. B.; Kim, K. T.; Kim, M. J.; Kim, S. H.; Kim, Y. J.; Kinoshita, K.; Kodyš, P.; Korpar, S.; Kotchetkov, D.; Križan, P.; Krokovny, P.; Kuhr, T.; Kulasiri, R.; Kumar, R.; Kumita, T.; Kuzmin, A.; Kwon, Y. -J.; Lange, J. S.; Lee, I. S.; Li, C. H.; Li, L.; Li, Y.; Li Gioi, L.; Libby, J.; Liventsev, D.; Lubej, M.; Luo, T.; Masuda, M.; Matvienko, D.; Merola, M.; Miyabayashi, K.; Miyata, H.; Mizuk, R.; Mohanty, G. B.; Mohanty, S.; Moon, H. K.; Mori, T.; Mussa, R.; Nakano, E.; Nakao, M.; Nanut, T.; Nath, K. J.; Natkaniec, Z.; Nayak, M.; Niiyama, M.; Nisar, N. K.; Nishida, S.; Ogawa, S.; Okuno, S.; Ono, H.; Pakhlov, P.; Pakhlova, G.; Pal, B.; Pardi, S.; Park, C. -S.; Park, H.; Paul, S.; Pedlar, T. K.; Pesántez, L.; Pestotnik, R.; Piilonen, L. E.; Prasanth, K.; Ritter, M.; Rostomyan, A.; Sahoo, H.; Sakai, Y.; Sandilya, S.; Santelj, L.; Sanuki, T.; Sato, Y.; Savinov, V.; Schneider, O.; Schnell, G.; Schwanda, C.; Schwartz, A. J.; Seino, Y.; Senyo, K.; Sevior, M. E.; Shebalin, V.; Shen, C. P.; Shibata, T. -A.; Shiu, J. -G.; Shwartz, B.; Simon, F.; Sokolov, A.; Solovieva, E.; Starič, M.; Strube, J. F.; Stypula, J.; Sumisawa, K.; Sumiyoshi, T.; Takizawa, M.; Tamponi, U.; Tanida, K.; Tenchini, F.; Uchida, M.; Uglov, T.; Unno, Y.; Uno, S.; Urquijo, P.; Usov, Y.; Van Hulse, C.; Varner, G.; Vorobyev, V.; Vossen, A.; Waheed, E.; Wang, C. H.; Wang, M. -Z.; Wang, P.; Watanabe, M.; Watanabe, Y.; Widmann, E.; Williams, K. M.; Won, E.; Yamashita, Y.; Ye, H.; Yelton, J.; Yook, Y.; Yuan, C. Z.; Yusa, Y.; Zhang, Z. P.; Zhilich, V.; Zhukova, V.; Zhulanov, V.; Zupanc, A.

    2017-10-01

    We report a study of the decay <mi>D>0<mi>KS>0<mi>KS>0 using 921 fb-1 of data collected at or near the Υ(4S) and Υ(5S) resonances with the Belle detector at the KEKB asymmetric energy e+e- collider. The measured time-integrated CP asymmetry is ACP(<mi>D>0<mi>KS>0<mi>KS>0) = (-0.02 ± 1.53 ± 0.02 ± 0.17)%, and the branching fraction is B(<mi>D>0<mi>KS>0<mi>KS>0) = (1.321 ± 0.023 ± 0.036 ± 0.044) × 10-4, where the first uncertainty is statistical, the second is systematic, and the third is due to the normalization mode (<mi>D>0<mi>KS>0π0). These results are significantly more precise than previous measurements available for this mode. The ACP measurement is consistent with the standard model expectation.

  4. Ground-penetrating radar and electromagnetic surveys at the Monroe Crossroads battlefield site, Fort Bragg, North Carolina

    Science.gov (United States)

    Kessler, Richard; Strain, R.E.; Marlowe, J. I.; Currin, K.B.

    1996-01-01

    A ground-penetrating radar survey was conducted at the Monroe Crossroads Battlefield site at Fort Bragg, North Carolina, to determine possible locations of subsurface archaeological features. An electromagnetic survey also was conducted at the site to verify and augment the ground-penetrating radar data. The surveys were conducted over a 67,200-square-foot grid with a grid point spacing of 20 feet. During the ground-penetrating radar survey, 87 subsurface anomalies were detected based on visual inspection of the field records. These anomalies were flagged in the field as they appeared on the ground-penetrating radar records and were located by a land survey. The electromagnetic survey produced two significant readings at ground-penetrating radar anomaly locations. The National Park Service excavated 44 of the 87 anomaly locations at the Civil War battlefield site. Four of these excavations produced significant archaeological features, including one at an abandoned well.

  5. From "Lawrence Kohlberg's Approach to Moral Education" by F. Clark Power, Ann Higgins, and Lawrence Kohlberg, with Judy Codding (1989)

    Science.gov (United States)

    Schools: Studies in Education, 2011

    2011-01-01

    This article is an excerpt from "Lawrence Kohlberg's Approach to Moral Education." It refers several times to Kohlberg's "six stages of moral development." Stages 3 and 4 belong to the second level of moral development, which Kohlberg calls "conventional." At stage 3, one becomes aware of conventions as one sees what is right in terms of living up…

  6. The Art of the Possible: T. E. Lawrence and Coalition Liaison

    Science.gov (United States)

    2001-06-01

    Letters of T. E. Lawrence and his Brothers ed. M. R . Lawrence (Oxford: Blackwell; New York: MacMillan, 1954), 284; quoted in Jeremy Wilson, Lawrence of...and dysentery and was bedridden in his tent for ten days. Up to this point in the fighting things had been moving quickly and Lawrence had been, by...determines that information is subject to special dissemination limitation specified by paragraph 4-505, DoD 5200.1- R . STATEMENT X: Distribution

  7. MiDAS

    DEFF Research Database (Denmark)

    McIlroy, Simon Jon; Saunders, Aaron Marc; Albertsen, Mads

    2015-01-01

    The Microbial Database for Activated Sludge (MiDAS) field guide is a freely available online resource linking the identity of abundant and process critical microorganisms in activated sludge wastewater treatment systems to available data related to their functional importance. Phenotypic properties...... of some of these genera are described, but most are known only from sequence data. The MiDAS taxonomy is a manual curation of the SILVA taxonomy that proposes a name for all genus-level taxa observed to be abundant by large-scale 16 S rRNA gene amplicon sequencing of full-scale activated sludge...... communities. The taxonomy can be used to classify unknown sequences, and the online MiDAS field guide links the identity to the available information about their morphology, diversity, physiology and distribution. The use of a common taxonomy across the field will provide a solid foundation for the study...

  8. 77 FR 42642 - Safety Zone; City of Ogdensburg Fireworks, St. Lawrence River, Ogdensburg, NY

    Science.gov (United States)

    2012-07-20

    ...-AA00 Safety Zone; City of Ogdensburg Fireworks, St. Lawrence River, Ogdensburg, NY AGENCY: Coast Guard... portion of the St. Lawrence River during the City of Ogdensburg Fireworks display. This temporary safety... as follows: Sec. 165.T09-0608 Safety Zone; City of Ogdensburg Fireworks, St. Lawrence River...

  9. the contribution made by te lawrence to the theory of revolutionary

    African Journals Online (AJOL)

    Lawrence was basically an academic thrown into the hurly-burly of leading an Arab revolt against. Turkish domination. It could be said that the war in the Middle East was a sideshow of the First. World War and Lawrence's part was a ' ... sideshow to the sideshow'l) Why then has Lawrence been remembered when greater ...

  10. 77 FR 38488 - Safety Zone; Alexandria Bay Chamber of Commerce, St. Lawrence River, Alexandria Bay, NY

    Science.gov (United States)

    2012-06-28

    ... 1625-AA00 Safety Zone; Alexandria Bay Chamber of Commerce, St. Lawrence River, Alexandria Bay, NY... restrict vessels from a portion of the St. Lawrence River during the Alexandria Bay Chamber of Commerce... of proposed rulemaking (NPRM) entitled Safety Zone; Alexandria Bay Chamber of Commerce, St. Lawrence...

  11. Safety analysis report for packaging Lawrence Livermore Laboratories shipping containers

    International Nuclear Information System (INIS)

    Evans, J.H.

    1975-12-01

    The Lawrence Livermore Laboratories shipping containers were designed at Oak Ridge National Laboratory for use in transporting weapons and nuclear components. The design for the containers was evaluated to show compliance with applicable regulations governing packages in which radioactive and fissile materials are transported. Computational procedures were used to determine the structural integrity and thermal behavior of the containers relative to the standards for the normal conditions of transport. A full-scale container test model was destructively tested to verify compliance with the standards for the accident conditions. The results of the analytical evaluations and the tests demonstrate that the design for the Lawrence Livermore Laboratories shipping containers is in compliance with the applicable regulations

  12. Seismic evaluation of critical facilities at the Lawrence Livermore Laboratory

    International Nuclear Information System (INIS)

    Murray, R.C.; Tokarz, F.J.

    1976-01-01

    The performance of critical facilities at the Lawrence Livermore Laboratory (LLL) are being evaluated for severe earthquake loading. Facilities at Livermore, Site-300 and the Nevada Test Site are included in this study. These facilities are identified, the seismic criteria used for the analysis are indicated, the various methods used for structural analysis are discussed and a summary of the results of facilities analyzed to date are presented

  13. Annual site environmental report of the Lawrence Berkeley Laboratory

    International Nuclear Information System (INIS)

    Schleimer, G.E.; Pauer, R.O.

    1991-05-01

    The Environmental Monitoring Program of the Lawrence Berkeley Laboratory is described. Data for 1990 are presented, and general trends are discussed. The report is organized under the following topics: Environmental Program Overview; Environmental Permits; Environmental Assessments; Environmental Activities; Penetrating Radiation; Airborne Radionuclides; Waterborne Radionuclides; Public Doses Resulting from LBL Operations; Trends -- LBL Environmental Impact; Waterborne Pollutants; Airborne Pollutants; Groundwater Protection; and Quality Assurance. 20 refs., 26 figs., 23 tabs

  14. Catalog of research projects at Lawrence Berkeley Laboratory, 1985

    International Nuclear Information System (INIS)

    1985-01-01

    This Catalog has been created to aid in the transfer of technology from the Lawrence Berkeley Laboratory to potential users in industry, government, universities, and the public. The projects are listed for the following LBL groups: Accelerator and Fusion Research Division, Applied Science Division, Biology and Medicine Division, Center for Advanced Materials, Chemical Biodynamics Division, Computing Division, Earth Sciences Division, Engineering and Technical Services Division, Materials and Molecular Research Division, Nuclear Science Division, and Physics Division

  15. Aerial radiological survey of the Lawrence Livermore Laboratory (Livermore, California)

    International Nuclear Information System (INIS)

    Tipton, W.J.

    1977-10-01

    An airborne radiological survey was conducted during August 1975 over several selected sites in the vicinity of Livermore, California. These sites included the Lawrence Livermore Laboratory, Sandia Livermore Laboratories, LLL Site 300, the Livermore Municipal Golf Course, and the City of Livermore's sewage treatment plant. The radiation results were processed specifically for man-made gamma ray activity. All elevated man-made activity observed during the aerial survey was contained within the site boundaries of the three DOE facilities

  16. Guide to user facilities at the Lawrence Berkeley Laboratory

    International Nuclear Information System (INIS)

    1984-04-01

    Lawrence Berkeley Laboratories' user facilities are described. Specific facilities include: the National Center for Electron Microscopy; the Bevalac; the SuperHILAC; the Neutral Beam Engineering Test Facility; the National Tritium Labeling Facility; the 88 inch Cyclotron; the Heavy Charged-Particle Treatment Facility; the 2.5 MeV Van de Graaff; the Sky Simulator; the Center for Computational Seismology; and the Low Background Counting Facility

  17. Catalog of research projects at Lawrence Berkeley Laboratory, 1985

    Energy Technology Data Exchange (ETDEWEB)

    1985-01-01

    This Catalog has been created to aid in the transfer of technology from the Lawrence Berkeley Laboratory to potential users in industry, government, universities, and the public. The projects are listed for the following LBL groups: Accelerator and Fusion Research Division, Applied Science Division, Biology and Medicine Division, Center for Advanced Materials, Chemical Biodynamics Division, Computing Division, Earth Sciences Division, Engineering and Technical Services Division, Materials and Molecular Research Division, Nuclear Science Division, and Physics Division.

  18. Treasury Offset Program (TOP) MI

    Data.gov (United States)

    Social Security Administration — The TOP MI helps OPSOS coordinate TOP case processing in the regions. The MI also helped communicate our progress and findings to BFQM and ORDP, as well as the ACOSS.

  19. Mi-spillet

    DEFF Research Database (Denmark)

    Larsen, Lea Lund; Hejlesen, Stine

    2003-01-01

    MI-spillet er et undervisningsspil til folkeskolens mellemtrin og udskolingen. Spillet omformer Howard Gardners teori om de mange intelligenser til et praktisk og håndgribeligt værktøj til brug i folkeskolen. Spillet indeholder et undervisningsmateriale bestående af lærervejledning og kopimappe...... emnebaseret eller tværfagligt arbejde. Alt materialet ligger samlet på en cd-rom, hvorfra materialet printes. Skolen kan derfor ved køb af én cd-rom printe og producere et ubegrænset antal spil. Cd-rommen indeholder: 1. Lærervejledning 2. MI-spillet * Gulvpladerne * Spørgsmål til spillet * Bilag til...

  20. Seismic strengthening of building 111 at Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    Eli, M.; Coats, D.; Freeland, G.; Kamath, M.

    1991-01-01

    Since being designed and constructed in the late 1960s, the Director's Building (Building 111) at Lawrence Livermore National Laboratory (LLNL) has been evaluated for 1988 seismic criteria and has been upgraded to withstand a major earthquake in the Livermore area. During and immediately after a large earthquake in the Livermore area, Building 111 occupants would be able to exit safely without loss of life. Building 111 itself would be severely damaged, but would not collapse. Highlights of the seismic upgrade design criteria and of the design, analyses, and construction that resulted are presented in this paper

  1. Electromagnetic wiggler technology development at the Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    Deis, G.A.; Burns, M.J.; Christensen, T.C.; Coffield, F.E.; Kulke, B.; Prosnitz, D.; Scharlemann, E.T.; Halbach, K.

    1987-01-01

    As a part of the program at the Lawrence Livermore National Laboratory (LLNL) in induction-linac free-electron laser (IFEL) research, we are conducting a variety of activities addressing the unique requirements imposed on IFEL wiggler systems. We are actively developing improved dc iron-core electromagnetic wiggler designs to attain higher peak fields, greater tunability, and lower random error levels. We are pursuing specialized control systems, such as magnetic-field and beam-position controllers, which can relax requirements on the wiggler itself. We are also pursuing basic studies to establish the effect of radiation on permanent magnets

  2. Lawrence Berkeley National Laboratory 1995 site environmental report

    Energy Technology Data Exchange (ETDEWEB)

    Balgobin, D.; Javandel, I.; Lackner, G.; Smith, C.; Thorson, P.; Tran, H.

    1996-07-01

    The 1995 Site Environmental Report summarizes environmental activities at the Ernest Orlando Lawrence Berkeley National Laboratory (LBNL) for the 1995 calendar year. The report strives to present environmental data in a manner that characterizes the performance and compliance status of the environmental management programs. The report also discusses significant highlights and plans of these programs. Topics discussed include: environmental monitoring, environmental compliance programs, air quality, water quality, ground water protection, sanitary sewer monitoring, soil and sediment quality, vegetation and foodstuffs monitoring, and special studies which include preoperational monitoring of building 85 and 1995 sampling results, radiological dose assessment, and quality assessment.

  3. Stabilization of plutonium bearing residues at Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    Bronson, M.C.; Van Konynenburg, R.A.; Ebbinghaus, B.B.

    1995-01-01

    The US Department of Energy's (US DOE) Lawrence Livermore National Laboratory (LLNL) has plutonium holdings including metal, oxide and residue materials, all of which need stabilization of some type. Residue materials include calcined ash, calcined precipitates, pyrochemical salts, glove box sweepings, metallurgical samples, graphite, and pyrochemical ceramic crucibles. These residues are typical of residues stored throughout the US DOE plutonium sites. The stabilization process selected for each of these residues requires data on chemical impurities, physical attributes, and chemical forms of the plutonium. This paper outlines the characterization and stabilization of LLNL ash residues, pyrochemical salts, and graphite

  4. Annual environmental monitoring report of the Lawrence Berkeley Laboratory

    International Nuclear Information System (INIS)

    Schleimer, G.E.

    1989-06-01

    The Environmental Monitoring Program of the Lawrence Berkeley Laboratory (LBL) is described. Data for 1988 are presented and general trends are discussed. In order to establish whether LBL research activities produced any impact on the population surrounding the laboratory, a program of environmental air and water sampling and continuous radiation monitoring was carried on throughout the year. For 1988, as in the previous several years, dose equivalents attributable to LBL radiological operations were a small fraction of both the relevant radiation protection guidelines (RPG) and of the natural radiation background. 16 refs., 7 figs., 21 tabs

  5. Pyrochemical processing automation at Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    Dennison, D.K.; Domning, E.E.; Seivers, R.

    1991-01-01

    Lawrence Livermore National Laboratory (LLNL) is developing a fully automated system for pyrochemical processing of special nuclear materials (SNM). The system utilizes a glove box, an automated tilt-pour furnace (TPF), an IBM developed gantry robot, and specialized automation tooling. All material handling within the glove box (i.e., furnace loading, furnace unloading, product and slag separation, and product packaging) is performed automatically. The objectives of the effort are to increase process productivity, decrease operator radiation, reduce process wastes, and demonstrate system reliability and availability. This paper provides an overview of the automated system hardware, outlines the overall operations sequence, and discusses the current status

  6. Lawrence Berkeley National Laboratory 1995 site environmental report

    International Nuclear Information System (INIS)

    Balgobin, D.; Javandel, I.; Lackner, G.; Smith, C.; Thorson, P.; Tran, H.

    1996-07-01

    The 1995 Site Environmental Report summarizes environmental activities at the Ernest Orlando Lawrence Berkeley National Laboratory (LBNL) for the 1995 calendar year. The report strives to present environmental data in a manner that characterizes the performance and compliance status of the environmental management programs. The report also discusses significant highlights and plans of these programs. Topics discussed include: environmental monitoring, environmental compliance programs, air quality, water quality, ground water protection, sanitary sewer monitoring, soil and sediment quality, vegetation and foodstuffs monitoring, and special studies which include preoperational monitoring of building 85 and 1995 sampling results, radiological dose assessment, and quality assessment

  7. Annual environmental monitoring report of the Lawrence Berkeley Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Schleimer, G.E. (ed.)

    1989-06-01

    The Environmental Monitoring Program of the Lawrence Berkeley Laboratory (LBL) is described. Data for 1988 are presented and general trends are discussed. In order to establish whether LBL research activities produced any impact on the population surrounding the laboratory, a program of environmental air and water sampling and continuous radiation monitoring was carried on throughout the year. For 1988, as in the previous several years, dose equivalents attributable to LBL radiological operations were a small fraction of both the relevant radiation protection guidelines (RPG) and of the natural radiation background. 16 refs., 7 figs., 21 tabs.

  8. Technical Safety Appraisal of the Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    1990-12-01

    This report documents the results of the Technical Safety Appraisal (TSA) of the Lawrence Livermore National Laboratory (LLNL) (including the Site 300 area), Livermore, California, conducted from February 26 to April 5, 1990. The purpose of the assessment was to provide the Secretary of Energy with the status of Environment, Safety and Health (ES H) Programs at LLNL. LLNL is operated by the University of California for the Department of Energy (DOE), and is a multi-program, mission-oriented institution engaged in fundamental and applied research programs that require a multidisciplinary approach. 1 fig.

  9. Lawrence Berkeley Laboratory upgrading approaches to existing facilities

    International Nuclear Information System (INIS)

    Engle, H.M. Jr.

    1985-01-01

    The Lawrence Berkeley Laboratory Plant Engineering Department instituted a seismic risk investigation and seismic upgrade program in 1970. This paper covers the upgrade of two buildings with dissimilar framing systems; Building No. 10, a World War II vintage heavy timber frame building, and Building No. 80, a steel frame structure constructed in 1954. The seismic upgrade task for both structures required that the buildings be kept in service during rehabilitation with a minimum of disruption to occupants. Rehabilitations were phased over two and three year periods with construction management and supervision performed by LBL Plant Engineering staff

  10. Natura e morte al tempo della guerra secondo Lawrence

    Directory of Open Access Journals (Sweden)

    Maria Grazia Dongu

    2017-11-01

    Full Text Available While the propaganda language strived to promote the Great War as a fight against evil forces, the event loomed soon above English people as an anticipation of the death of Western civilization. Images of death recur in the short story “England, my England” by D. H. Lawrence. Egbert’s death stands for the death of Old England and its value system, but also for the intellectuals’ failure to speak out and mould a new language, which could defy the propaganda. In the end, the rural world is abandoned by the characters. Egbert embraces dissolution as a relief from the agony of his own world.

  11. Lawrence Livermore National Laboratory seismic yield determination for the NPE

    Energy Technology Data Exchange (ETDEWEB)

    Rohrer, R. [Lawrence Livermore National Lab., CA (United States)

    1994-12-31

    The Lawrence Livermore National Laboratory recorded seismic signals from the Non-Proliferation experiment at the Nevada Test Site on September 22, 1993, at seismic stations near Mina, Nevada; Kanab Utah; Landers, California; and Elko, Nevada. Yields were calculated from these recorded seismic amplitudes at the stations using statistical amplitude- yield regression curves from earlier nuclear experiments performed near the Non-Proliferation experiment. The weighted seismic yield average using these amplitudes is 1.9 kt with a standard deviation of 19%. The calibrating experiments were nuclear, so this yield is equivalent to a 1.9-kt nuclear experiment.

  12. Diagnostic and prognostic potential of serum miR-7, miR-16, miR-25, miR-93, miR-182, miR-376a and miR-429 in ovarian cancer patients.

    Science.gov (United States)

    Meng, Xiaodan; Joosse, Simon A; Müller, Volkmar; Trillsch, Fabian; Milde-Langosch, Karin; Mahner, Sven; Geffken, Maria; Pantel, Klaus; Schwarzenbach, Heidi

    2015-11-03

    Owing to late diagnosis in advanced disease stages, prognosis of patients with epithelial ovarian cancer (EOC) is poor. The quantification of deregulated levels of microRNAs could facilitate earlier diagnosis and improve prognosis of EOC. Seven microRNAs (miR-7, miR-16, miR-25, miR-93, miR-182, miR-376a and miR-429) were quantified in the serum of 180 EOC patients and 66 healthy women by TaqMan PCR microRNA assays. Median follow-up time was 21 months. The effects of miR-7 and miR-429 on apoptosis, cell proliferation, migration and invasion were investigated in two (EOC) cell lines. Serum levels of miR-25 (P=0.0001) and miR-93 (P=0.0001) were downregulated, whereas those of miR-7 (P=0.001) and miR-429 (P=0.0001) were upregulated in EOC patients compared with healthy women. The four microRNAs discriminated EOC patients from healthy women with a sensitivity of 93% and a specificity of 92%. The levels of miR-429 positively correlated with CA125 values (P=0.0001) and differed between FIGO I-II and III-IV stages (P=0.001). MiR-429 was an independent predictor of overall survival (P=0.011). Overexpressed miR-429 in SKOV3 cells led to suppression of cell migration (P=0.037) and invasion (P=0.011). Increased levels of miR-7 were associated with lymph node metastases (P=0.0001) and FIGO stages III-IV (P=0.0001). Overexpressed miR-7 in SKOV3 cells resulted in increased cell migration (P=0.001) and invasion (P=0.011). Additionally, the increased levels of miR-376a correlated with FIGO stages III-IV (P=0.02). Our data indicate the diagnostic potential of miR-7, miR-25, miR-93 and miR-429 in EOC and the prognostic potential of miR-429. This microRNA panel may be promising molecules to be targeted in the treatment of EOC.

  13. Reclaiming Sámi languages

    DEFF Research Database (Denmark)

    Rasmussen, Torkel; Nolan, John Shaun

    2011-01-01

    , this paper investigates what subsequently happens at the grassroots or micro level. This investigation shows that despite more positive policies, there is a strong sentiment of defeatism with regard to Sámi. Sámi speakers face problems because of the lack of implementation of nationally decided laws...... and for the sake of cultural maintenance, but also for instrumental reasons, i.e. to give their children better opportunities in the labor market where knowledge of Sámi is necessary....

  14. MiDAS

    DEFF Research Database (Denmark)

    McIlroy, Simon Jon; Kirkegaard, Rasmus Hansen; McIlroy, Bianca

    A deep understanding of the microbial communities and dynamics in wastewater treatment systems is a powerful tool for process optimization and design (Rittmann et al., 2006). With the advent of amplicon sequencing of the 16S rRNA gene, the diversity within the microbial communities can now...... web platform about the microbes in activated sludge and their associated ADs. The MiDAS taxonomy proposes putative names for each genus-level-taxon that can be used as a common vocabulary for all researchers in the field....

  15. The Advanced Light Source at Lawrence Berkeley Laboratory

    International Nuclear Information System (INIS)

    Robinson, A.L.; Perera, R.C.C.; Schlachter, A.S.

    1991-10-01

    The Advanced Light Source (ALS) at the Lawrence Berkeley Laboratory (LBL), scheduled to be operational in the spring of 1993 as a US Department of Energy national user facility, will be a next- generation source of soft x-ray and ultraviolet (XUV) synchrotron radiation. Undulators will provide the world's brightest synchrotron radiation at photon energies from below 10 eV to above 2 keV; wiggler and bend-magnet radiation will extend the spectral coverage with high fluxes above 10 keV. These capabilities will support an extensive research program in a broad spectrum of scientific and technological areas in which XUV radiation is used to study and manipulate matter in all its varied gaseous, liquid, and solid forms. The ALS will also serve those interested in developing the fabrication technology for micro- and nanostructures, as well as characterizing them

  16. Lawrence Livermore National Laboratory laser-fusion program

    International Nuclear Information System (INIS)

    Ahlstrom, H.G.

    1982-01-01

    The goals of the Laser-Fusion Program at Lawrence Livermore National Laboratory are to produce well-diagnosed, high-gain, laser-driven fusion explosions in the laboratory and to exploit this capability for both military applications and for civilian energy production. In the past year we have made significant progress both theoretically and experimentally in our understanding of the laser interaction with both directly coupled and radiation-driven implosion targets and their implosion dynamics. We have made significant developments in fabricating the target structures. Data from the target experiments are producing important near-term physics results. We have also continued to develop attractive reactor concepts which illustrate ICF's potential as an energy producer

  17. Absolute instrumental neutron activation analysis at Lawrence Livermore Laboratory

    International Nuclear Information System (INIS)

    Heft, R.E.

    1977-01-01

    The Environmental Science Division at Lawrence Livermore Laboratory has in use a system of absolute Instrumental Neutron Activation Analysis (INAA). Basically, absolute INAA is dependent upon the absolute measurement of the disintegration rates of the nuclides produced by neutron capture. From such disintegration rate data, the amount of the target element present in the irradiated sample is calculated by dividing the observed disintegration rate for each nuclide by the expected value for the disintegration rate per microgram of the target element that produced the nuclide. In absolute INAA, the expected value for disintegration rate per microgram is calculated from nuclear parameters and from measured values of both thermal and epithermal neutron fluxes which were present during irradiation. Absolute INAA does not depend on the concurrent irradiation of elemental standards but does depend on the values for thermal and epithermal neutron capture cross-sections for the target nuclides. A description of the analytical method is presented

  18. The Lawrence Livermore National Laboratory Intelligent Actinide Analysis System

    International Nuclear Information System (INIS)

    Buckley, W.M.; Carlson, J.B.; Koenig, Z.M.

    1993-07-01

    The authors have developed an Intelligent Actinide Analysis System (IAAS) for Materials Management to use in the Plutonium Facility at the Lawrence Livermore National Laboratory. The IAAS will measure isotopic ratios for plutonium and other actinides non-destructively by high-resolution gamma-ray spectrometry. This system will measure samples in a variety of matrices and containers. It will provide automated control of many aspects of the instrument that previously required manual intervention and/or control. The IAAS is a second-generation instrument, based on the authors' experience in fielding gamma isotopic systems, that is intended to advance non-destructive actinide analysis for nuclear safeguards in performance, automation, ease of use, adaptability, systems integration and extensibility to robotics. It uses a client-server distributed monitoring and control architecture. The IAAS uses MGA 3 as the isotopic analysis code. The design of the IAAS reduces the need for operator intervention, operator training, and operator exposure

  19. Ambient environmental radiation monitoring at the Lawrence Livermore Laboratory

    International Nuclear Information System (INIS)

    Lindeken, C.L.; White, J.H.; Toy, A.J.; Sundbeck, C.W.

    1975-01-01

    Thermoluminescence dosimetry is the principal means of measuring ambient γ radiation at the Lawrence Livermore Laboratory. These dosimeters are used at 12 perimeter locations and 41 locations in the off-site vicinity of the Laboratory, and are exchanged quarterly. Control dosimeters are stored in a 75-mm-thick lead shield located out-of-doors to duplicate temperature cycling of field dosimeters. Effect of dosimeter response to radiation in the shield is determined each quarter. Calibration irradiations are made midway through the exposure cycle to compensate for signal fading. Terrestrial exposure rates calculated from the activities of naturally occurring uranium, thorium, and potassium in Livermore Valley soils vary from 3 to 7 μR/hr. Local inferred exposure rates from cosmic radiation are approximately 4 μR/hr. TLD measurements are in good agreement with these data. Off-site and site perimeter data are compared, and differences related to Laboratory operations are discussed

  20. Malignant melanoma among employees of Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    Austin, D.F.; Reynolds, P.J.; Snyder, M.A.; Biggs, M.W.; Stubbs, H.A.

    1981-01-01

    19 cases of malignant melanoma (MM) were observed during 1972-77 among approximately 5100 employees of the Lawrence Livermore National Laboratory, where high energy physics research is conducted. This number was significantly higher (p -6 ) than that expected in a comparable age/race/sex/geographical segment of the population of the San Francisco Bay area. That excess seemed to occur only among laboratory employees and not among the surrounding community, which suggests that an occupational factor is responsible. Preliminary case-comparison findings suggest that MM risk is not associated with length of employment at the laboratory nor with type of monitored radiation exposure. Although the data did not support an association between MM incidence and all scientific job classifications combined, an excess relative risk was observed among chemists. The reasons for the MM excess have not been identified. (author)

  1. Environmental restoration at the Lawrence Livermore National Laboratory Livermore Site

    International Nuclear Information System (INIS)

    Ziagos, J.P.; Bainer, R.W.; Dresen, M.D.; Hoffman, J.D.

    1992-04-01

    Ground water beneath Lawrence Livermore National Laboratory (LLNL) near Livermore California, contains 19 compounds in concentrations exceeding regulatory standards. These include volatile organic compounds (VOCs), dissolved fuel hydrocarbons, free product gasoline, cadmium, chromium, lead, and tritium. VOCs are the most widespread hazardous materials in the ground water, covering an area of about 1.4 square miles. The other compounds occur sporadically around the site. The LLNL site was added to the National Priorities (Superfund) List in 1987. This paper describes the technology developed at LLNL to remediate soil and ground water contamination. Included in this paper are methods in which site characterization has been aided by using a drilling technique developed at LLNL to evaluate the vertical distribution of VOCs in multiple water-bearing zones in single borehole. The paper also describes the development and implementation of a comprehensive three-step program to investigate and evaluate potential sources of hazardous materials in soil and ground water

  2. Environmental surveillance program of the Lawrence Berkeley Laboratory

    International Nuclear Information System (INIS)

    Thomas, R.H.

    1976-04-01

    The major radiological environmental impact of the Lawrence Berkeley Laboratory is due to the operation of four particle accelerators. Potential sources of population exposure at the Laboratory are discussed. The major source of population exposure due to accelerator operation arises from the prompt radiation field which consists principally of neutrons and photons. Release of small quantities of radionuclides is also a potential source of population exposure but is usually an order of magnitude less significant. Accelerator produced radiation levels at the Laboratory boundary are comparable with the magnitudes of the fluctuations found in the natural background radiation. Environmental monitoring of accelerator-produced radiation and of radionuclides is carried on throughout the Laboratory, at the Laboratory perimeter, and in the regions surrounding the Laboratory. The techniques used are described. The models used to calculate population exposure are described and discussed

  3. Images of Turks in the Works of Lawrence Durrell

    Directory of Open Access Journals (Sweden)

    Ahmet Kayıntu

    2011-12-01

    Full Text Available This study seeks to explore the images of Turks as a non-European other in the works of Lawrence Durrell. In his works, Durrell shows how representations of cultural difference are inextricably linked to representations of sexual difference. Thus Durrell‟s Europeans are surrounded with an aura of colonial power, erotic potency, and easy penetration into the spaces of the other while Turks are represented as feminized, emasculated and humiliated. The images of the European White man and non- are dependent on the negative image of Turks, the other. In exactly the same way, Istanbul is presented as a city of decadence and decay, a place where decent human values broken down and where individuals survive by their wits ability to dodge or swindle

  4. Optical Design Capabilities at Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    Lawson, J.K.

    2002-01-01

    Optical design capabilities continue to play the same strong role at Lawrence Livermore National Laboratory (LLNL) that they have played in the past. From defense applications to the solid-state laser programs to the Atomic Vapor Laser Isotope Separation (AVLIS), members of the optical design group played critical roles in producing effective system designs and are actively continuing this tradition. This talk will explain the role optical design plays at LLNL, outline current capabilities and summarize a few activities in which the optical design team has been recently participating. Among the many optical engineers working at LLNL, a distinct group exists which specializes in optical design issues. The optical design group collectively has a wide range of fields of expertise as well as a diversity of background histories including LLNL, university, industry and aerospace experience. This unique resource has resulted many effective and productive designs for customers at LLNL and outside the lab.

  5. Lawrence Livermore Laboratory concept for uranium recovery from seawater

    International Nuclear Information System (INIS)

    Gregg, D.; Wang, F.

    1980-01-01

    The Lawrence Livermore Laboratory concept for uranium recovery from seawater involves the following process steps: (1) produce activated carbon via a coal gasification plant; (2) contact activated carbon sorbent with seawater using a settling process (no pumping of seawater); (3) vacuum activated carbon from sea floor; (4) gasify or burn activated carbon (further concentrating the uranium in the ash); (5) extract the uranium from the rich ash ore by conventional techniques. The process advantages are: (1) eliminates seawater pumping, the need for an illuent, and the need for a fresh water wash; (2) should result in much lower capital investment and regional process energy. Major process issues are: (1) uranium loading on activated carbon; (2) activated carbon modifications required to improve the sorbtion performance; (3) activated carbon particle size needed to meet system requirements; (4) minimization of sorbent losses when contacted with seawater

  6. The Lawrence Livermore National Laboratory Intelligent Actinide Analysis System

    International Nuclear Information System (INIS)

    Buckley, W.M.; Carlson, J.B.; Koenig, Z.M.

    1993-01-01

    The authors have developed an Intelligent Actinide Analysis System (IAAS) for Materials Management to use in the Plutonium Facility at the Lawrence Livermore National Laboratory. The IAAS will measure isotopic ratios for plutonium and other actinides non-destructively by high-resolution gamma-ray spectrometry. This system will measure samples in a variety of matrices and containers. It will provide automated control of many aspects of the instrument that previously required manual intervention and/or control. The IAAS is a second-generation instrument, based on experience in fielding gamma isotopic systems, that is intended to advance non-destructive actinide analysis for nuclear safeguards in performance, automation, ease of use, adaptability, systems integration and extensibility to robotics. It uses a client-server distributed monitoring and control architecture. The IAAS uses MGA as the isotopic analysis code. The design of the IAAS reduces the need for operator intervention, operator training, and operator exposure

  7. Lawrence Livermore National Laboratory environmental report for 1990

    International Nuclear Information System (INIS)

    Sims, J.M.; Surano, K.A.; Lamson, K.C.; Balke, B.K.; Steenhoven, J.C.; Schwoegler, D.R.

    1990-01-01

    This report documents the results of the Environmental Monitoring Program at the Lawrence Livermore National Laboratory (LLNL) and presents summary information about environmental compliance for 1990. To evaluate the effect of LLNL operations on the local environment, measurements of direct radiation and a variety of radionuclides and chemical compounds in ambient air, soil, sewage effluent surface water, groundwater, vegetation, and foodstuff were made at both the Livermore site and at Site 300 nearly. LLNL's compliance with all applicable guides, standards, and limits for radiological and nonradiological emissions to the environment was evaluated. Aside from an August 13 observation of silver concentrations slightly above guidelines for discharges to the sanitary sewer, all the monitoring data demonstrated LLNL compliance with environmental laws and regulations governing emission and discharge of materials to the environment. In addition, the monitoring data demonstrated that the environmental impacts of LLNL are minimal and pose no threat to the public to or to the environment. 114 refs., 46 figs., 79 tabs

  8. Lawrence Livermore National Laboratory (LLNL) Waste Minimization Program Plan

    International Nuclear Information System (INIS)

    Heckman, R.A.; Tang, W.R.

    1989-01-01

    This Program Plan document describes the background of the Waste Minimization field at Lawrence Livermore National Laboratory (LLNL) and refers to the significant studies that have impacted on legislative efforts, both at the federal and state levels. A short history of formal LLNL waste minimization efforts is provided. Also included are general findings from analysis of work to date, with emphasis on source reduction findings. A short summary is provided on current regulations and probable future legislation which may impact on waste minimization methodology. The LLN Waste Minimization Program Plan is designed to be dynamic and flexible so as to meet current regulations, and yet is able to respond to an everchanging regulatory environment. 19 refs., 12 figs., 8 tabs

  9. Seip-lawrence Syndrome (Three Cases in a Family

    Directory of Open Access Journals (Sweden)

    B.S.N. Reddy

    1986-01-01

    Full Text Available A, rare episode of Seip-Lawrence syndrome manifesting in all three case siblings of consanpinous parents is reported. Two children we′re male and one female. They exhibited low intelligence,′ gaunt facies, depressed bridge of nose, large low-sct ears, thick lips and protruberant abdomen. Skin was showing hypermelanosis, hypertrichosis, absence of subcutaneous fat and acan nigricaFNx01s with′ very prominent perianal rUgO6itiS In addition, the first child was short statured having hypertrophic Clitoris, hepatomcoy, left ventricular hypertro hy, hyperglycaemia and glycossuria- without ketoacidosis. The second child was, having enlargement of penis, left ventricular hypertrophy,,hepatospienomegaly and abnormal GTT. The third and the youngest child was having only cutaneous changes and no viscoromegaly or biochemical abnormality. Nou Of these patients were having gigantism and advanced bone age.

  10. Estonia's defence dollars spent wisely? / Tony Lawrence, Kaarel Kaas ; interv. Joel Alas

    Index Scriptorium Estoniae

    Lawrence, Tony

    2007-01-01

    Rahvusvahelise Kaitseuuringute Keskuse teadurid Tony Lawrence ja Kaarel Kaas kommenteerivad Eestis Suurbritannia kaitseatasheena töötanud kolonelleitnant Glen Granti kriitikat kaitsejõudude efektiivsuse osas, Eesti kaitsepoliitikat, küberrünnakut Eestile, kahe Eesti rahukaitseväelase surma missioonil Afganistanis ning üldsuse suhtumist Eesti osalemisele rahvusvahelistel missioonidel. Lisa: Tony Lawrence; Kaarel Kaas

  11. 75 FR 78335 - Culturally Significant Objects Imported for Exhibition Determinations: “Thomas Lawrence: Regency...

    Science.gov (United States)

    2010-12-15

    ... DEPARTMENT OF STATE [Public Notice 7268] Culturally Significant Objects Imported for Exhibition Determinations: ``Thomas Lawrence: Regency Power and Brilliance'' SUMMARY: Notice is hereby given of the... determine that the objects to be included in the exhibition ``Thomas Lawrence: Regency Power and Brilliance...

  12. Lawrence Kohlberg, una obra en permanente construcción Lawrence Kohlberg, a Work in Permanent Construction

    Directory of Open Access Journals (Sweden)

    Jaime Yánez-Canal

    2012-06-01

    Full Text Available Resumen Este artículo, resultado de investigación documental del grupo Estudios sobre el Desarrollo Sociomoral, presenta un análisis sobre la historia y la evolución intelectual de Lawrece Kohlberg, pionero de la Psicología del Desarrollo Moral. Específicamente, el análisis de su obra gira alrededor de tres tópicos: la filosofía moral, la psicología del desarrollo y la pedagogía.Abstract This paper, documental research outcome of the ''Estudios sobre el Desarrollo Sociomoral'' research group, presents an analysis of the history and intellectual evolution of Lawrence Kohlberg, pioneer of Moral Development Psychology. Specifically, the analysis of his work revolves around three issues: moral Philosophy, developmental Psychology and Pedagogy.

  13. Berkeley Lab's Saul Perlmutter wins E.O. Lawrence Award; scientist's work on supernovae reveals accelerating Universe

    CERN Multimedia

    2002-01-01

    Saul Perlmutter, from Lawrence Berkeley National Laboratory Physics Division and leader of the Supernova Cosmology Project based there, has won the DOE's 2002 E.O. Lawrence Award in the physics category (2 pages).

  14. 75 FR 8428 - The Indiana Rail Road Company-Abandonment Exemption-in Martin and Lawrence Counties, IN; CSX...

    Science.gov (United States)

    2010-02-24

    ...; Richard Wilson, on behalf of Radius Indiana; Citizens Against Rails-to-Trails; and several landowners..., Lawrence County Tourism Commission Executive Director; Gene McCracken, Lawrence County Economic Growth...

  15. Expression profiling of miR-96, miR-584 and miR-422a in colon ...

    African Journals Online (AJOL)

    Purpose: To determine the correlation between miRNAs; miR-96, miR-422a and miR584, and colon cancer, and also to test whether any of these miRNAs can act as non-invasive biomarkers in colon cancer. Methods: The tumor samples and the corresponding normal mucosa used in this study were collected from 60 ...

  16. Generation of miRNA sponge constructs

    NARCIS (Netherlands)

    Kluiver, Joost; Slezak-Prochazka, Izabella; Smigielska-Czepiel, Katarzyna; Halsema, Nancy; Kroesen, Bart-Jan; van den Berg, Anke

    2012-01-01

    MicroRNA (miRNA) sponges are RNA molecules with repeated miRNA antisense sequences that can sequester miRNAs from their endogenous targets and thus serve as a decoy. Stably expressed miRNA sponges are especially valuable for long-term loss-of-function studies and can be used in vitro and in vivo. We

  17. 78 FR 71037 - St. Lawrence & Atlantic Railroad Company-Discontinuance of Service Exemption-in Cumberland County...

    Science.gov (United States)

    2013-11-27

    ... DEPARTMENT OF TRANSPORTATION Surface Transportation Board [Docket No. AB 1117X] St. Lawrence..., 2013, St. Lawrence & Atlantic Railroad Company (SLR) filed with the Surface Transportation Board (Board..., FD 35440 (STB served Dec. 29, 2010); Maine--Acquisition Exemption--Certain Assets of St. Lawrence...

  18. Tiger Team assessment of the Lawrence Berkeley Laboratory, Washington, DC

    Energy Technology Data Exchange (ETDEWEB)

    1991-02-01

    This report documents the results of the Department of Energy's (DOE's) Tiger Team Assessment of the Lawrence Berkeley Laboratory (LBL) conducted from January 14 through February 15, 1991. The purpose of the assessment was to provide the Secretary of Energy with the status of environment, safety, and health (ES H) programs at LBL. The Tiger Team concluded that curtailment of cessation of any operations at LBL is not warranted. However, the number and breadth of findings and concerns from this assessment reflect a serious condition at this site. In spite of its late start, LBL has recently made progress in increasing ES H awareness at all staff levels and in identifying ES H deficiencies. Corrective action plans are inadequate, however, many compensatory actions are underway. Also, LBL does not have the technical expertise or training programs nor the tracking and followup to effectively direct and control sitewide guidance and oversight by DOE of ES H activities at LBL. As a result of these deficiencies, the Tiger Team has reservations about LBL's ability to implement effective actions in a timely manner and, thereby, achieve excellence in their ES H program. 4 figs., 24 tabs.

  19. Lawrence Livermore Laboratory Nuclear Test Effects and Geologic Data Bank

    International Nuclear Information System (INIS)

    Howard, N.W.

    1976-01-01

    Data on the geology of the USERDA Nevada Test Site have been collected for the purpose of evaluating the possibility of release of radioactivity at proposed underground nuclear test sites. These data, including both the rock physical properties and the geologic structure and stratigraphy of a large number of drill-hole sites, are stored in the Lawrence Livermore Laboratory Earth Sciences Division Nuclear Test Effects and Geologic Data Bank. Retrieval programs can quickly provide a geological and geophysical comparison of a particular site with other sites where radioactivity was successfully contained. The data can be automatically sorted, compared, and averaged, and information listed according to site location, drill-hole construction, rock units, depth to key horizons and to the water table, and distance to faults. These programs also make possible ordered listings of geophysical properties (interval bulk density, overburden density, interval velocity, velocity to the surface, grain density, water content, carbonate content, porosity, and saturation of the rocks). The characteristics and capabilities of the data bank are discussed

  20. Tiger Team assessment of the Lawrence Berkeley Laboratory, Washington, DC

    International Nuclear Information System (INIS)

    1991-02-01

    This report documents the results of the Department of Energy's (DOE's) Tiger Team Assessment of the Lawrence Berkeley Laboratory (LBL) conducted from January 14 through February 15, 1991. The purpose of the assessment was to provide the Secretary of Energy with the status of environment, safety, and health (ES ampersand H) programs at LBL. The Tiger Team concluded that curtailment of cessation of any operations at LBL is not warranted. However, the number and breadth of findings and concerns from this assessment reflect a serious condition at this site. In spite of its late start, LBL has recently made progress in increasing ES ampersand H awareness at all staff levels and in identifying ES ampersand H deficiencies. Corrective action plans are inadequate, however, many compensatory actions are underway. Also, LBL does not have the technical expertise or training programs nor the tracking and followup to effectively direct and control sitewide guidance and oversight by DOE of ES ampersand H activities at LBL. As a result of these deficiencies, the Tiger Team has reservations about LBL's ability to implement effective actions in a timely manner and, thereby, achieve excellence in their ES ampersand H program. 4 figs., 24 tabs

  1. Lawrence Livermore National Laboratory Working Reference Material Production Pla

    Energy Technology Data Exchange (ETDEWEB)

    Wong, Amy; Thronas, Denise; Marshall, Robert

    1998-11-04

    This Lawrence Livermore National Laboratory (LLNL) Working Reference Material Production Plan was written for LLNL by the Los Alamos National Laboratory to address key elements of producing seven Pu-diatomaceous earth NDA Working Reference Materials (WRMS). These WRMS contain low burnup Pu ranging in mass from 0.1 grams to 68 grams. The composite Pu mass of the seven WRMS was designed to approximate the maximum TRU allowable loading of 200 grams Pu. This document serves two purposes: first, it defines all the operations required to meet the LLNL Statement of Work quality objectives, and second, it provides a record of the production and certification of the WRMS. Guidance provided in ASTM Standard Guide C1128-89 was used to ensure that this Plan addressed all the required elements for producing and certifying Working Reference Materials. The Production Plan was written to provide a general description of the processes, steps, files, quality control, and certification measures that were taken to produce the WRMS. The Plan identifies the files where detailed procedures, data, quality control, and certification documentation and forms are retained. The Production Plan is organized into three parts: a) an initial section describing the preparation and characterization of the Pu02 and diatomaceous earth materials, b) middle sections describing the loading, encapsulation, and measurement on the encapsulated WRMS, and c) final sections describing the calculations of the Pu, Am, and alpha activity for the WRMS and the uncertainties associated with these quantities.

  2. Comparison of St. Lawrence blue whale vocalizations with field observations

    Science.gov (United States)

    Berchok, Catherine; Bradley, David; Gabrielson, Thomas; Sears, Richard

    2003-04-01

    During four field seasons from 1998-2001, vocalizations were recorded in the presence of St. Lawrence blue whales using a single omni-directional hydrophone. Both long duration infrasonic calls (~18 Hz, 5-20 s) as well as short duration higher frequency calls (85-25 Hz, ~2 s) were detected and compared with field observations. Two trends were noted. First, the long infrasonic call series were concentrated primarily in the deep (300 m) channel. These call series appear to compare well with blue whale vocalizations recorded by others in the deep open ocean. Second, the shorter audible calls were more evenly distributed over bathymetry and seem to be a form of short distance communication with at least one case occurring during an agonistic interaction. A comparison of these calls with biological parameters such as density of whales in the area, percentages of paired versus single whales, and numbers of males versus females will also be discussed. [Project supported by ARL/PSU, NSF, and the American Museum of Natural History.

  3. STS-114 Crew Interviews: 1. Eileen Collins 2. Wendy Lawrence

    Science.gov (United States)

    2005-01-01

    1) STS-114 Commander Eileen Collins emphasized her love for teaching, respect for teachers, and her plan to go back to teaching again someday. Her solid background in Math and Science, focus on her interests, with great support from her family, and great training and support during her career with the Air Force gave her confidence in pursuing her dream to become an astronaut. Commander Collins shares her thoughts on the Columbia, details the various flight operations and crew tasks that will take place during the mission and the importance of Shuttle missions to the International Space Station and space exploration. 2) STS-114 Mission Specialist Wendy Lawrence first dreamed of becoming an astronaut when she watched Neil Armstrong walk on the moon from their black and white TV set. She majored in Engineering and became a Navy pilot. She shares her thoughts on the Columbia, details her major role as the crew in charge of all the transfer operations; getting the MPLM unpacked and repacked; and the importance of Shuttle missions to the International Space Station and space exploration.

  4. Environmental Survey preliminary report, Lawrence Livermore National Laboratory, Livermore, California

    Energy Technology Data Exchange (ETDEWEB)

    1987-12-01

    This report presents the preliminary findings from the first phase of the Environmental Survey of the Department of Energy (DOE) Lawrence Livermore National Laboratory (LLNL), conducted December 1 through 19, 1986. The Survey is being conducted by an interdisciplinary team of environmental specialists, led and managed by the Office of Environment, Safety and Health's Office of Environmental Audit. Individual team components are being supplied by a private contractor. The objective of the Survey is to identify environmental problems and areas of environmental risk associated with LLNL. The Survey covers all environmental media all areas of environmental regulation. It is being performed in accordance with the DOE Environmental Survey Manual. This phase of the Survey involves the review of existing site environmental data, observations of the operations performed at LLNL, and interviews with site personnel. A Sampling and Analysis Plan was developed to assist in further assessing certain of the environmental problems identified during performance of on-site activities. The Sampling and Analysis Plan will be executed by a DOE National Laboratory. When completed, the results will be incorporated into the LLNL Environmental Survey Interim Report. The Interim Report will reflect the final determinations of the LLNL Survey. 70 refs., 58 figs., 52 tabs.,

  5. Annual environmental monitoring report of the Lawrence Berkeley Laboratory, 1977

    International Nuclear Information System (INIS)

    Stephens, L.D.

    1978-03-01

    The data obtained from the Environmental Monitoring Program of the Lawrence Berkeley Laboratory for the Calendar year 1977 are described and general trends are discussed. The general trend of decreasing radiation levels at our site boundary due to accelerator operation during past years has leveled off during 1977 and in some areas shows a slight but not statistically significant increase as predicted in last year's summary. There were changes in both ion beams as well as current which have resulted in shifts in maxima at the monitoring stations. The gamma levels are once again reported as zero. There is only one period of detectable gamma radiation due to accelerator operation. The annual dose equivalent are reported from the environmental monitoring stations since they have been established. Radiation levels at the Olympus Gate Station have shown a steady decline since 1959 when estimates were first made. The Olympus Gate Station is in direct view of the Bevatron and most directly influenced by that accelerator. Over the past several years the atmospheric sampling program has, with the exception of occasional known releases, yielded data which are within the range of normal background. The surface water program always yields results within the range of normal background. As no substantial changes in the quantities of radionuclides used are anticipated, no changes are expected in these observations

  6. Environmental Survey preliminary report, Lawrence Berkeley Laboratory, Berkeley, California

    International Nuclear Information System (INIS)

    1988-07-01

    The purpose of this report is to present the preliminary findings made during the Environmental Survey, February 22--29, 1988, at the US Department of Energy (DOE) Lawrence Berkeley Laboratory (LBL) in Berkeley, California. The University of California operates the LBL facility for DOE. The LBL Survey is part of the larger DOE-wide Environmental Survey announced by Secretary John S. Herrington on September 18, 1985. The purpose of this effort is to identify, via ''no fault'' baseline Surveys, existing environmental problems and areas of environmental risk at DOE facilities, and to rank them on a DOE wide basis. This ranking will enable DOE to more effectively establish priorities for addressing environmental problems and allocate the resources necessary to correct them. Because the Survey is ''no fault'' and is not an ''audit,'' it is not designed to identify specific isolated incidents of noncompliance or to analyze environmental management practices. Such incidents and/or management practices will, however, be used in the Survey as a means of identifying existing and potential environmental problems. The LBL Survey was conducted by a multidisciplinary team of technical specialists headed and managed by a Team Leader and Assistant Team Leader from DOE's Office of Environmental Audit. A complete list of the LBL Survey participants and their affiliations is provided in Appendix A. 80 refs., 27 figs., 37 tabs

  7. Lawrence Livermore National Laboratory environmental report for 1990

    Energy Technology Data Exchange (ETDEWEB)

    Sims, J.M.; Surano, K.A.; Lamson, K.C.; Balke, B.K.; Steenhoven, J.C.; Schwoegler, D.R. (eds.)

    1990-01-01

    This report documents the results of the Environmental Monitoring Program at the Lawrence Livermore National Laboratory (LLNL) and presents summary information about environmental compliance for 1990. To evaluate the effect of LLNL operations on the local environment, measurements of direct radiation and a variety of radionuclides and chemical compounds in ambient air, soil, sewage effluent surface water, groundwater, vegetation, and foodstuff were made at both the Livermore site and at Site 300 nearly. LLNL's compliance with all applicable guides, standards, and limits for radiological and nonradiological emissions to the environment was evaluated. Aside from an August 13 observation of silver concentrations slightly above guidelines for discharges to the sanitary sewer, all the monitoring data demonstrated LLNL compliance with environmental laws and regulations governing emission and discharge of materials to the environment. In addition, the monitoring data demonstrated that the environmental impacts of LLNL are minimal and pose no threat to the public to or to the environment. 114 refs., 46 figs., 79 tabs.

  8. LLNL (Lawrence Livermore National Laboratory) research on cold fusion

    Energy Technology Data Exchange (ETDEWEB)

    Thomassen, K I; Holzrichter, J F [eds.

    1989-09-14

    With the appearance of reports on Cold Fusion,'' scientists at the Lawrence Livermore National Laboratory (LLNL) began a series of increasingly sophisticated experiments and calculations to explain these phenomena. These experiments can be categorized as follows: (a) simple experiments to replicate the Utah results, (b) more sophisticated experiments to place lower bounds on the generation of heat and production of nuclear products, (c) a collaboration with Texas A M University to analyze electrodes and electrolytes for fusion by-products in a cell producing 10% excess heat (we found no by-products), and (d) attempts to replicate the Frascati experiment that first found neutron bursts when high-pressure deuterium gas in a cylinder with Ti chips was temperature-cycled. We failed in categories (a) and (b) to replicate either the Pons/Fleischmann or the Jones phenomena. We have seen phenomena similar to the Frascati results, (d) but these low-level burst signals may not be coming from neutrons generated in the Ti chips. Summaries of our experiments are described in Section II, as is a theoretical effort based on cosmic ray muons to describe low-level neutron production. Details of the experimental groups' work are contained in the six appendices. At LLNL, independent teams were spontaneously formed in response to the early announcements on cold fusion. This report's format follows this organization.

  9. Protection planning and risk management at Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    Hunt, J.S.; Altman, W.D.; Hockert, J.W.

    1988-01-01

    Effective safeguards and security management begins with comprehensive strategic planning that synthesizes protection objectives, threat information, existing protection capabilities, consequences of protection failure, and the costs and impacts of safeguards changes into cost effective protection strategies that adequately address credible threats. Lawrence Livermore National Laboratory (LLNL) has developed a structured risk management approach to safeguards and security planning that is designed to lead to protection strategies that are cost effective, meet the intent of Department of Energy (DOE) orders, balance protection needs with programmatic priorities, and acknowledge a level of residual risks that is not cost effective to eliminate. This risk management approach to safeguards decision making was used to develop the first DOE-approved Master Safeguards and Security Agreement (MSSA) that addresses all security interests at a major facility including: special nuclear material, classified information and materials, computer and communication security, and other DOE property. This risk management approach also provides the strategic basis for day-to-day management of the LLNL security program as well as the integration of safeguards program upgrades

  10. Reuse of waste cutting sand at Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    Mathews, S.; Wilson, K.

    1998-01-01

    Lawrence Livermore National Laboratory (LLNL) examined the waste stream from a water jet cutting operation, to evaluate the possible reuse of waste garnet sand. The sand is a cutting agent used to shape a variety of materials, including metals. Nearly 70,000 pounds of waste sand is generated annually by the cutting operation. The Environmental Protection Department evaluated two potential reuses for the spent garnet sand: backfill in utility trenches; and as a concrete constituent. In both applications, garnet waste would replace the sand formerly purchased by LLNL for these purposes. Findings supported the reuse of waste garnet sand in concrete, but disqualified its proposed application as trench backfill. Waste sand stabilized in a concrete matrix appeared to present no metals-leaching hazard; however, unconsolidated sand in trenches could potentially leach metals in concentrations high enough to threaten ground water quality. A technical report submitted to the San Francisco Bay Regional Water Quality Control Board was reviewed and accepted by that body. Reuse of waste garnet cutting sand as a constituent in concrete poured to form walkways and patios at LLNL was approved

  11. BUDAYA LONGKO' TORAJA DALAM PERSPEKTIF ETIKA LAWRENCE KOHLBERG

    Directory of Open Access Journals (Sweden)

    Diks Sasmanto Pasande

    2013-08-01

    Full Text Available This research studies one of the local wisdoms in Toraja society which is longko' or culture of shame. This culture contains values of religious ethics and communal spirit to maintain self-esteem and vigilance in order to be not humiliated/ shamed (kalongkoran. The longko' of Toraja is observed from the ethics perspective of Lawrence Kohlberg to analyze ethical values of the culture. The result of this research indicates that, according to the Kohlberg's theory of moral development stages, Toraja society tends to be at the conventional level and the third stages which is characterized by a social morality where the union with familiar groups becomes the highest value. It is in accordance with communal ties which are very strong in the Toraja society and known as tongkonan. The longko', as an internalization result of understanding varieties which is held steadfastly by the Toraja society, contains not only values of honor, self esteem and shame, but also other positive values such as work ethos and spirit. Kamalamburan (honesty and karapasan (harmony and peace are the main values of Toraja society. The Longko' is quite relevant, especially in relation to the national efforts to solve the problem of corruption through the cultural approach.

  12. Environmental Survey preliminary report, Lawrence Berkeley Laboratory, Berkeley, California

    Energy Technology Data Exchange (ETDEWEB)

    1988-07-01

    The purpose of this report is to present the preliminary findings made during the Environmental Survey, February 22--29, 1988, at the US Department of Energy (DOE) Lawrence Berkeley Laboratory (LBL) in Berkeley, California. The University of California operates the LBL facility for DOE. The LBL Survey is part of the larger DOE-wide Environmental Survey announced by Secretary John S. Herrington on September 18, 1985. The purpose of this effort is to identify, via no fault'' baseline Surveys, existing environmental problems and areas of environmental risk at DOE facilities, and to rank them on a DOE wide basis. This ranking will enable DOE to more effectively establish priorities for addressing environmental problems and allocate the resources necessary to correct them. Because the Survey is no fault'' and is not an audit,'' it is not designed to identify specific isolated incidents of noncompliance or to analyze environmental management practices. Such incidents and/or management practices will, however, be used in the Survey as a means of identifying existing and potential environmental problems. The LBL Survey was conducted by a multidisciplinary team of technical specialists headed and managed by a Team Leader and Assistant Team Leader from DOE's Office of Environmental Audit. A complete list of the LBL Survey participants and their affiliations is provided in Appendix A. 80 refs., 27 figs., 37 tabs.

  13. Lawrence Berkeley National Laboratory 1997 Site Environmental Report Vol. I

    International Nuclear Information System (INIS)

    Thorson, Patrick

    1998-01-01

    Each year, Ernest Orlando Lawrence Berkeley National Laboratory prepares an integrated report on its environmental programs to satisfy the requirements of U.S. Department of Energy Order 231.1. The Site Environmental Report for 1997 is intended to summarize Berkeley Lab's compliance with environmental standards and requirements, characterize environmental management efforts through surveillance and monitoring activities, and highlight significant programs and efforts for calendar year 1997. This report is structured into three basic areas that cover a general overview of the Laboratory, the status of environmental programs, and the results of the surveillance and monitoring activities, including air quality, surface water, groundwater, sanitary sewer, soil and sediment, vegetation and foodstuffs, radiation dose assessment, and quality assurance. The report is separated into two volumes. Volume I contains the body of the report, a list of references, a list of acronyms and abbreviations, a glossary, Appendix A (NESHAPS annual report), and Appendix B (distribution list for volume I). Volume II contains Appendix C, the individual data results from monitoring programs. Each chapter in volume I begins with an outline of the sections that follow

  14. Earthquake safety program at Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    Freeland, G.E.

    1985-01-01

    Within three minutes on the morning of January 24, 1980, an earthquake and three aftershocks, with Richter magnitudes of 5.8, 5.1, 4.0, and 4.2, respectively, struck the Livermore Valley. Two days later, a Richter magnitude 5.4 earthquake occurred, which had its epicenter about 4 miles northwest of the Lawrence Livermore National Laboratory (LLNL). Although no one at the Lab was seriously injured, these earthquakes caused considerable damage and disruption. Masonry and concrete structures cracked and broke, trailers shifted and fell off their pedestals, office ceilings and overhead lighting fell, and bookcases overturned. The Laboratory was suddenly immersed in a site-wide program of repairing earthquake-damaged facilities, and protecting our many employees and the surrounding community from future earthquakes. Over the past five years, LLNL has spent approximately $10 million on its earthquake restoration effort for repairs and upgrades. The discussion in this paper centers upon the earthquake damage that occurred, the clean-up and restoration efforts, the seismic review of LLNL facilities, our site-specific seismic design criteria, computer-floor upgrades, ceiling-system upgrades, unique building seismic upgrades, geologic and seismologic studies, and seismic instrumentation. 10 references

  15. Lawrence Berkeley National Laboratory 2015 Annual Financial Report

    Energy Technology Data Exchange (ETDEWEB)

    Williams, Kim, P

    2017-08-11

    FY2015 financial results reflect a year of significant scientific, operational and financial achievement for Lawrence Berkeley National Laboratory. Complementing many scientific accomplishments, Berkeley Lab completed construction of four new research facilities: the General Purpose Laboratory, Chu Hall, Wang Hall and the Flexlab Building Efficiency Testbed. These state-of-the-art facilities allow for program growth and enhanced collaboration, in part by enabling programs to return to the Lab’s Hill Campus from offsite locations. Detailed planning began for the new Integrative Genomics Building (IGB) that will house another major program currently located offsite. Existing site infrastructure was another key focus area. The Lab prioritized and increased investments in deferred maintenance in alignment with the Berkeley Lab Infrastructure Plan, which was developed under the leadership of the DOE Office of Science. With the expiration of American Recovery and Reinvestment Act (ARRA) funds, we completed the close-out of all of our 134 ARRA projects, recording total costs of $331M over the FY2009-2015 period. Download the report to read more.

  16. Interactions of miR-323/miR-326/miR-329 and miR-130a/miR-155/miR-210 as prognostic indicators for clinical outcome of glioblastoma patients

    Directory of Open Access Journals (Sweden)

    Qiu Shuwei

    2013-01-01

    Full Text Available Abstract Background Glioblastoma multiforme (GBM is the most common and aggressive brain tumor with poor clinical outcome. Identification and development of new markers could be beneficial for the diagnosis and prognosis of GBM patients. Deregulation of microRNAs (miRNAs or miRs is involved in GBM. Therefore, we attempted to identify and develop specific miRNAs as prognostic and predictive markers for GBM patient survival. Methods Expression profiles of miRNAs and genes and the corresponding clinical information of 480 GBM samples from The Cancer Genome Atlas (TCGA dataset were downloaded and interested miRNAs were identified. Patients’ overall survival (OS and progression-free survival (PFS associated with interested miRNAs and miRNA-interactions were performed by Kaplan-Meier survival analysis. The impacts of miRNA expressions and miRNA-interactions on survival were evaluated by Cox proportional hazard regression model. Biological processes and network of putative and validated targets of miRNAs were analyzed by bioinformatics. Results In this study, 6 interested miRNAs were identified. Survival analysis showed that high levels of miR-326/miR-130a and low levels of miR-323/miR-329/miR-155/miR-210 were significantly associated with long OS of GBM patients, and also showed that high miR-326/miR-130a and low miR-155/miR-210 were related with extended PFS. Moreover, miRNA-323 and miRNA-329 were found to be increased in patients with no-recurrence or long time to progression (TTP. More notably, our analysis revealed miRNA-interactions were more specific and accurate to discriminate and predict OS and PFS. This interaction stratified OS and PFS related with different miRNA levels more detailed, and could obtain longer span of mean survival in comparison to that of one single miRNA. Moreover, miR-326, miR-130a, miR-155, miR-210 and 4 miRNA-interactions were confirmed for the first time as independent predictors for survival by Cox regression model

  17. Beyond Lawrence v. Texas: crafting a fundamental right to sexual privacy.

    Science.gov (United States)

    Fasullo, Kristin

    2009-05-01

    After the watershed 2003 U.S. Supreme Court decision Lawrence v.Texas, courts are faced with the daunting task of navigating the bounds of sexual privacy in light of Lawrence's sweeping language and unconventional structure. This Note focuses on the specific issue of state governments regulating sexual device distribution. Evaluating the substantive due process rights of sexual device retailers and users, this Note ultimately argues that the privacy interest identified in Lawrence is sufficiently broad to protect intimate decisions to engage in adult consensual sexual behavior, including the liberty to sell, purchase, and use a sexual device.

  18. Clinical relevance of microRNA miR-21, miR-31, miR-92a, miR-101, miR-106a and miR-145 in colorectal cancer

    International Nuclear Information System (INIS)

    Schee, Kristina; Boye, Kjetil; Abrahamsen, Torveig Weum; Fodstad, Øystein; Flatmark, Kjersti

    2012-01-01

    MicroRNAs (miRNAs) regulate gene expression by binding to mRNA, and can function as oncogenes or tumor suppressors depending on the target. In this study, using qRT-PCR, we examined the expression of six miRNAs (miR-21, miR-31, miR-92a, miR-101, miR-106a and miR-145) in tumors from 193 prospectively recruited patients with colorectal cancer, and associations with clinicopathological parameters and patient outcome were analyzed. The miRNAs were chosen based on previous studies for their biomarker potential and suggested biological relevance in colorectal cancer. The miRNA expression was examined by qRT-PCR. Associations between miRNA expression and clinicopathological variables were explored using Mann–Whitney U and Kruskal-Wallis test while survival was estimated using the Kaplan-Meier method and compared using the log-rank test. MiR-101 was hardly expressed in the tumor samples, while for the other miRNAs, variable expression levels and expression ranges were observed, with miR-21 being most abundantly expressed relative to the reference (RNU44). In our study cohort, major clinical significance was demonstrated only for miR-31, as high expression was associated with advanced tumor stage and poor differentiation. No significant associations were found between expression of the investigated miRNAs and metastasis-free or overall survival. Investigating the expression of six miRNAs previously identified as candidate biomarkers in colorectal cancer, few clinically relevant associations were detected in our patient cohort. Our results emphasize the importance of validating potential tumor markers in independent patient cohorts, and indicate that the role of miRNAs as colorectal cancer biomarkers is still undetermined

  19. miREE: miRNA recognition elements ensemble

    Science.gov (United States)

    2011-01-01

    Background Computational methods for microRNA target prediction are a fundamental step to understand the miRNA role in gene regulation, a key process in molecular biology. In this paper we present miREE, a novel microRNA target prediction tool. miREE is an ensemble of two parts entailing complementary but integrated roles in the prediction. The Ab-Initio module leverages upon a genetic algorithmic approach to generate a set of candidate sites on the basis of their microRNA-mRNA duplex stability properties. Then, a Support Vector Machine (SVM) learning module evaluates the impact of microRNA recognition elements on the target gene. As a result the prediction takes into account information regarding both miRNA-target structural stability and accessibility. Results The proposed method significantly improves the state-of-the-art prediction tools in terms of accuracy with a better balance between specificity and sensitivity, as demonstrated by the experiments conducted on several large datasets across different species. miREE achieves this result by tackling two of the main challenges of current prediction tools: (1) The reduced number of false positives for the Ab-Initio part thanks to the integration of a machine learning module (2) the specificity of the machine learning part, obtained through an innovative technique for rich and representative negative records generation. The validation was conducted on experimental datasets where the miRNA:mRNA interactions had been obtained through (1) direct validation where even the binding site is provided, or through (2) indirect validation, based on gene expression variations obtained from high-throughput experiments where the specific interaction is not validated in detail and consequently the specific binding site is not provided. Conclusions The coupling of two parts: a sensitive Ab-Initio module and a selective machine learning part capable of recognizing the false positives, leads to an improved balance between

  20. Plant Monroe Sulfurmeter evaluation

    International Nuclear Information System (INIS)

    Ruby, J.D.; Wood, T.R.; Brown, D.R.

    1982-04-01

    The performance of an apparatus for measuring continuously the sulfur content of coal entering a 3000-MW electric generating power station is described. The apparatus, known as a Sulfurmeter, interrogates a sidestream of coal in a nondestructive fashion using prompt neutron activation analysis (PNAA). Preceding the Sulfurmeter is a coal-handling facility that blends a 1% S coal with a 3% S coal. The Sulfurmeter is used to evaluate the blend, which must meet an emission standard of 3.68 lb of SO 2 /10 6 Btu when fired. Sulfur measurements by the PNAA apparatus were compared with those from traditional ASTM chemistry - satisfactory agreement was obtained

  1. Community Relations Plan for Lawrence Berkeley Laboratory. Environmental Restoration Program

    Energy Technology Data Exchange (ETDEWEB)

    1993-07-01

    The Lawrence Berkeley Laboratory (LBL) has applied to the California Environmental Protection Agency, Department of Toxic Substances Control (DTSC), for renewal of its Hazardous Waste Handling Facility Permit. A permit is required under Resource Conservation and Recovery Act (RCRA) regulations. The permit will allow LBL to continue using its current hazardous waste handling facility, upgrade the existing facility, and construct a replacement facility. The new facility is scheduled for completion in 1995. The existing facility will be closed under RCRA guidelines by 1996. As part of the permitting process, LBL is required to investigate areas of soil and groundwater contamination at its main site in the Berkeley Hills. The investigations are being conducted by LBL`s Environmental Restoration Program and are overseen by a number of regulatory agencies. The regulatory agencies working with LBL include the California Environmental Protection Agency`s Department of Toxic Substances Control, the California Regional Water Quality Control Board, the Bay Area Air Quality Management District, the East Bay Municipal Utilities District, and the Berkeley Department of Environmental Health. RCRA requires that the public be informed of LBL`s investigations and site cleanup, and that opportunities be available for the public to participate in making decisions about how LBL will address contamination issues. LBL has prepared this Community Relations Plan (CRP) to describe activities that LBL will use to keep the community informed of environmental restoration progress and to provide for an open dialogue with the public on issues of importance. The CRP documents the community`s current concerns about LBL`s Environmental Restoration Program. Interviews conducted between February and April 1993 with elected officials, agency staff, environmental organizations, businesses, site neighbors, and LBL employees form the basis for the information contained in this document.

  2. Lawrence Livermore National Laboratory Probabilistic Seismic Hazard Codes Validation

    International Nuclear Information System (INIS)

    Savy, J B

    2003-01-01

    Probabilistic Seismic Hazard Analysis (PSHA) is a methodology that estimates the likelihood that various levels of earthquake-caused ground motion will be exceeded at a given location in a given future time-period. LLNL has been developing the methodology and codes in support of the Nuclear Regulatory Commission (NRC) needs for reviews of site licensing of nuclear power plants, since 1978. A number of existing computer codes have been validated and still can lead to ranges of hazard estimates in some cases. Until now, the seismic hazard community had not agreed on any specific method for evaluation of these codes. The Earthquake Engineering Research Institute (EERI) and the Pacific Engineering Earthquake Research (PEER) center organized an exercise in testing of existing codes with the aim of developing a series of standard tests that future developers could use to evaluate and calibrate their own codes. Seven code developers participated in the exercise, on a voluntary basis. Lawrence Livermore National laboratory participated with some support from the NRC. The final product of the study will include a series of criteria for judging of the validity of the results provided by a computer code. This EERI/PEER project was first planned to be completed by June of 2003. As the group neared completion of the tests, the managing team decided that new tests were necessary. As a result, the present report documents only the work performed to this point. It demonstrates that the computer codes developed by LLNL perform all calculations correctly and as intended. Differences exist between the results of the codes tested, that are attributed to a series of assumptions, on the parameters and models, that the developers had to make. The managing team is planning a new series of tests to help in reaching a consensus on these assumptions

  3. Lawrence Berkeley Laboratory Institutional Plan, FY 1993--1998

    Energy Technology Data Exchange (ETDEWEB)

    1992-10-01

    The FY 1993--1998 Institutional Plan provides an overview of the Lawrence Berkeley Laboratory mission, strategic plan, scientific initiatives, research programs, environment and safety program plans, educational and technology transfer efforts, human resources, and facilities needs. The Strategic Plan section identifies long-range conditions that can influence the Laboratory, potential research trends, and several management implications. The Initiatives section identifies potential new research programs that represent major long-term opportunities for the Laboratory and the resources required for their implementation. The Scientific and Technical Programs section summarizes current programs and potential changes in research program activity. The Environment, Safety, and Health section describes the management systems and programs underway at the Laboratory to protect the environment, the public, and the employees. The Technology Transfer and Education programs section describes current and planned programs to enhance the nation's scientific literacy and human infrastructure and to improve economic competitiveness. The Human Resources section identifies LBL staff composition and development programs. The section on Site and Facilities discusses resources required to sustain and improve the physical plant and its equipment. The Resource Projections are estimates of required budgetary authority for the Laboratory's ongoing research programs. The plan is an institutional management report for integration with the Department of Energy's strategic planning activities that is developed through an annual planning process. The plan identifies technical and administrative directions in the context of the National Energy Strategy and the Department of Energy's program planning initiatives. Preparation of the plan is coordinated by the Office for Planning and Development from information contributed by the Laboratory's scientific and support divisions.

  4. Lawrence Berkeley Laboratory Institutional Plan, FY 1993--1998

    Energy Technology Data Exchange (ETDEWEB)

    Chew, Joseph T.; Stroh, Suzanne C.; Maio, Linda R.; Olson, Karl R.; Grether, Donald F.; Clary, Mary M.; Smith, Brian M.; Stevens, David F.; Ross, Loren; Alper, Mark D.; Dairiki, Janis M.; Fong, Pauline L.; Bartholomew, James C.

    1992-10-01

    The FY 1993--1998 Institutional Plan provides an overview of the Lawrence Berkeley Laboratory mission, strategic plan, scientific initiatives, research programs, environment and safety program plans, educational and technology transfer efforts, human resources, and facilities needs. The Strategic Plan section identifies long-range conditions that can influence the Laboratory, potential research trends, and several management implications. The Initiatives section identifies potential new research programs that represent major long-term opportunities for the Laboratory and the resources required for their implementation. The Scientific and Technical Programs section summarizes current programs and potential changes in research program activity. The Environment, Safety, and Health section describes the management systems and programs underway at the Laboratory to protect the environment, the public, and the employees. The Technology Transfer and Education programs section describes current and planned programs to enhance the nation`s scientific literacy and human infrastructure and to improve economic competitiveness. The Human Resources section identifies LBL staff composition and development programs. The section on Site and Facilities discusses resources required to sustain and improve the physical plant and its equipment. The Resource Projections are estimates of required budgetary authority for the Laboratory`s ongoing research programs. The plan is an institutional management report for integration with the Department of Energy`s strategic planning activities that is developed through an annual planning process. The plan identifies technical and administrative directions in the context of the National Energy Strategy and the Department of Energy`s program planning initiatives. Preparation of the plan is coordinated by the Office for Planning and Development from information contributed by the Laboratory`s scientific and support divisions.

  5. Lawrence Berkeley Laboratory, Institutional Plan FY 1994--1999

    Energy Technology Data Exchange (ETDEWEB)

    1993-09-01

    The Institutional Plan provides an overview of the Lawrence Berkeley Laboratory mission, strategic plan, scientific initiatives, research programs, environment and safety program plans, educational and technology transfer efforts, human resources, and facilities needs. For FY 1994-1999 the Institutional Plan reflects significant revisions based on the Laboratory`s strategic planning process. The Strategic Plan section identifies long-range conditions that will influence the Laboratory, as well as potential research trends and management implications. The Initiatives section identifies potential new research programs that represent major long-term opportunities for the Laboratory, and the resources required for their implementation. The Scientific and Technical Programs section summarizes current programs and potential changes in research program activity. The Environment, Safety, and Health section describes the management systems and programs underway at the Laboratory to protect the environment, the public, and the employees. The Technology Transfer and Education programs section describes current and planned programs to enhance the nation`s scientific literacy and human infrastructure and to improve economic competitiveness. The Human Resources section identifies LBL staff diversity and development program. The section on Site and Facilities discusses resources required to sustain and improve the physical plant and its equipment. The new section on Information Resources reflects the importance of computing and communication resources to the Laboratory. The Resource Projections are estimates of required budgetary authority for the Laboratory`s ongoing research programs. The Institutional Plan is a management report for integration with the Department of Energy`s strategic planning activities, developed through an annual planning process.

  6. Status of the belugas of the St Lawrence estuary, Canada

    Directory of Open Access Journals (Sweden)

    Michael CS Kingsley

    2002-07-01

    Full Text Available A population of belugas (Delphinapterus leucas inhabiting the estuary of the St Lawrence river in Quebec, Canada, was depleted by unregulated hunting, not closed until 1979. Surveys in 1977 showed only a few hundred in the population. Surveys since then have produced increasing estimates of population indices. An estimate of the population, fully corrected for diving animals, was 1,238 (SE 119 in September 1997. The population was estimated to have increased from 1988 through 1997 by 31.4 belugas/yr (SE 13.1. Observations of population age structure, as well as data on age at death obtained from beach-cast carcasses, do not indicate serious problems at the population level, although there are indications that mortality of the oldest animals may be elevated. Few animals appear to live much over 30 years. From examination of beach-cast carcasses, it appears that most deaths are due to old age and disease; hunting is illegal, ship strikes and entrapments in fishing gear are rare, ice entrapments and predation are unknown. Among beach-cast carcasses recovered and necropsied, about 23% of the adults have malignant cancers, while most of the juveniles have pneumonia; other pathological conditions are diverse. No factors are known to be limiting numbers of this population. Habitat quality factors, including persistent contaminants, boat traffic and harassment, may affect the population’s rate of increase, but these effects have not been quantitatively evaluated. Comprehensive legislation exists with powers to protect the population and the environment of which it is a component, but application and enforcement of the laws is not without problems.

  7. Laser materials processing applications at Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    Hargrove, R.S.; Dragon, E.P.; Hackel, R.P.; Kautz, D.D.; Warner, B.E.

    1993-01-01

    High power and high radiance laser technologies developed at Lawrence Livermore National Laboratory (LLNL) such as copper-vapor lasers, solid-state slab lasers, dye lasers, harmonic wavelength conversion of these lasers, and fiber optic delivery systems show great promise for material processing tasks. Evaluation of models suggests significant potential for tenfold increases in welding, cutting, and drilling performance, as well as capability for applications in emerging technologies such as micromachining, surface treatment, and stereolithography. Copper and dye laser systems are currently being developed at LLNL for uranium enrichment production facilities. The goals of this program are to develop low-cost, reliable and maintainable industrial laser systems. Chains of copper lasers currently operate at more than 1.5 kW output and achieve mean time between failures of more than 1,000 hours. The beam quality of copper vapor lasers is approximately three times the diffraction limit. Dye lasers have near diffraction limited beam quality at greater than 1.0 kW. Diode laser pumped, Nd:YAG slab lasers are also being developed at LLNL. Current designs achieve powers of greater than 1.0 kW and projected beam quality is in the two to five times diffraction limited range. Results from cutting and drilling studies in titanium and stainless steel alloys show that cuts and holes with extremely fine features can be made with dye and copper-vapor lasers. High radiance beams produce low distortion and small heat-affected zones. The authors have accomplished very high aspect ratio holes in drilling tests (> 60: 1) and features with micron scale (5-50 μm) sizes. Other, traditionally more difficult, materials such as copper, aluminum and ceramics will soon be studied in detail

  8. Topectomy versus leukotomy: J. Lawrence Pool's contribution to psychosurgery.

    Science.gov (United States)

    Holland, Ryan; Kopel, David; Carmel, Peter W; Prestigiacomo, Charles J

    2017-09-01

    Surgery of the mind has a rather checkered past. Though its history begins with the prehistoric trephination of skulls to allow "evil spirits" to escape, the early- to mid-20th century saw a surge in the popularity of psychosurgery. The 2 prevailing operations were topectomy and leukotomy for the treatment of certain mental illnesses. Although they were modified and refined by several of their main practitioners, the effectiveness of and the ethics involved with these operations remained controversial. In 1947, Dr. J. Lawrence Pool and the Columbia-Greystone Associates sought to rigorously investigate the outcomes of specific psychosurgical procedures. Pool along with R. G. Heath and John Weber believed that nonexcessive bifrontal cortical ablation could successfully treat certain mental illnesses without the undesired consequences of irreversible personality changes. They conducted this investigation at the psychiatric hospital at Greystone Park near Morristown, New Jersey. Despite several encouraging findings of the Columbia-Greystone project, psychosurgery practices began to decline significantly in the 1950s. The uncertainty of results and ethical debates related to side effects made these procedures unpopular. Further, groups such as the National Association for the Advancement of Colored People and the American Civil Liberties Union condemned the use of psychosurgery, believing it to be an inhumane form of treatment. Today, there are strict guidelines that must be adhered to when evaluating a patient for psychosurgery procedures. It is imperative for the neurosurgery community to remember the history of psychosurgery to provide the best possible current treatment and to search for better future treatments for a particularly vulnerable patient population.

  9. Proposals for ORNL [Oak Ridge National Laboratory] support to Tiber LLNL [Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    Berry, L.A.; Rosenthal, M.W.; Saltmarsh, M.J.; Shannon, T.E.; Sheffield, J.

    1987-01-01

    This document describes the interests and capabilities of Oak Ridge National Laboratory in their proposals to support the Lawrence Livermore National Laboratory (LLNL) Engineering Test Reactor (ETR) project. Five individual proposals are cataloged separately. (FI)

  10. Reference miRNAs for miRNAome analysis of urothelial carcinomas.

    Directory of Open Access Journals (Sweden)

    Nadine Ratert

    Full Text Available BACKGROUND/OBJECTIVE: Reverse transcription quantitative real-time PCR (RT-qPCR is widely used in microRNA (miRNA expression studies on cancer. To compensate for the analytical variability produced by the multiple steps of the method, relative quantification of the measured miRNAs is required, which is based on normalization to endogenous reference genes. No study has been performed so far on reference miRNAs for normalization of miRNA expression in urothelial carcinoma. The aim of this study was to identify suitable reference miRNAs for miRNA expression studies by RT-qPCR in urothelial carcinoma. METHODS: Candidate reference miRNAs were selected from 24 urothelial carcinoma and normal bladder tissue samples by miRNA microarrays. The usefulness of these candidate reference miRNAs together with the commonly for normalization purposes used small nuclear RNAs RNU6B, RNU48, and Z30 were thereafter validated by RT-qPCR in 58 tissue samples and analyzed by the algorithms geNorm, NormFinder, and BestKeeper. PRINCIPAL FINDINGS: Based on the miRNA microarray data, a total of 16 miRNAs were identified as putative reference genes. After validation by RT-qPCR, miR-101, miR-125a-5p, miR-148b, miR-151-5p, miR-181a, miR-181b, miR-29c, miR-324-3p, miR-424, miR-874, RNU6B, RNU48, and Z30 were used for geNorm, NormFinder, and BestKeeper analyses that gave different combinations of recommended reference genes for normalization. CONCLUSIONS: The present study provided the first systematic analysis for identifying suitable reference miRNAs for miRNA expression studies of urothelial carcinoma by RT-qPCR. Different combinations of reference genes resulted in reliable expression data for both strongly and less strongly altered miRNAs. Notably, RNU6B, which is the most frequently used reference gene for miRNA studies, gave inaccurate normalization. The combination of four (miR-101, miR-125a-5p, miR-148b, and miR-151-5p or three (miR-148b, miR-181b, and miR-874

  11. 77 FR 30443 - Safety Zone; Alexandria Bay Chamber of Commerce, St. Lawrence River, Alexandria Bay, NY

    Science.gov (United States)

    2012-05-23

    ...The Coast Guard proposes to establish a temporary safety zone on the St. Lawrence River, Alexandria Bay, NY. This proposed rule is intended to restrict vessels from a portion of the St. Lawrence River during the Alexandria Bay Chamber of Commerce fireworks display. The safety zone established by this proposed rule is necessary to protect spectators and vessels from the hazards associated with a fireworks display.

  12. Scientists in Gray Flannel Suits: Ernest Lawrence and the Development of Color Television

    Science.gov (United States)

    Roebke, Joshua

    Physicists and historians typically remember Ernest Lawrence for one of two activities, his development of the cyclotron or his advocacy for atomic weapons. The two labs that he established in support of such endeavors are still named after him in California: Lawrence Berkeley and Lawrence Livermore. But there was a third accomplishment for which Lawrence believed he would always be remembered: the development of color television. In 1950, he sold a half stake of his company, Chromatic Television Laboratories, to Paramount Pictures for 1 million. That decade, Lawrence and his employees, especially Luis Alvarez and Edwin McMillan, designed cathode-ray tubes for color televisions while they championed hydrogen bombs. Although their commitment to the second was attributed to patriotism and their interest in the first was dismissed as a hobby, it is not so easy to disentangle their motives. Color screens were needed for more than variety shows and sitcoms; they displayed incoming missiles in vivid color. No company has ever been led by three future Nobel Laureates, yet Chromatic Television Laboratories was a failure. Even so, Lawrence had a profound influence on the development of color television, and I will tell this story for the first time.

  13. Anguilla rostrata glass eel migration and recruitment in the estuary and Gulf of St Lawrence.

    Science.gov (United States)

    Dutil, J-D; Dumont, P; Cairns, D K; Galbraith, P S; Verreault, G; Castonguay, M; Proulx, S

    2009-06-01

    This study describes catches of Anguilla rostrata glass eels and associated oceanographic conditions in the St Lawrence Estuary and Gulf. Ichthyoplankton survey data suggest that they enter the Gulf primarily in May, migrate at the surface at night, and disperse broadly once they have passed Cabot Strait. They arrive in estuaries beginning at about mid-June and through the month of July. Migration extends west up to Québec City, in the freshwater zone of the St Lawrence Estuary, 1000 km west of Cabot Strait. Anguilla rostrata glass eels travel between Cabot Strait and receiving estuaries at a straight-line ground speed of c. 10-15 km day(-1). Catches of fish per unit effort in estuaries in the St Lawrence system are much lower than those reported for the Atlantic coast of Canada. Low abundance of A. rostrata glass eels in the St Lawrence system may be due to cold surface temperatures during the migration period which decrease swimming capacity, long distances from the spawning ground to Cabot Strait and from Cabot Strait to the destination waters (especially the St Lawrence River), complex circulation patterns, and hypoxic conditions in bottom waters of the Laurentian Channel and the St Lawrence Estuary.

  14. Supplement Analysis for the Transmission System Vegetation Management Program FEIS (DOE/EIS-0285/SA-106)SnoKing Tap to Monroe-Samamish Line

    Energy Technology Data Exchange (ETDEWEB)

    Martin, Mark A. [Bonneville Power Administration, Portland, OR (United States)

    2002-09-30

    Vegetation Management along the SnoKing Tap to the Monroe-Samamish transmission line from structure 8/1 through structure 20/6. BPA proposes to remove unwanted vegetation along the right-of-way, access roads and around tower structures along the subject transmission line corridor. The right-of-way will be treated using selective and non-selective methods that include hand cutting, mowing and herbicide treatments. Approximately 12 miles of access roads will be cleared using selective and non-selective methods that include hand cutting, mowing and herbicide treatments. Approximately 65 tower sites will be treated using selective and non-selective methods that include hand cutting, mowing and herbicide treatments. Vegetation management is required for unimpeded operation and maintenance of the subject transmission line. See Section 1of the attached checklist for a complete description of the proposal.

  15. MiRNA Biogenesis and Intersecting Pathways

    DEFF Research Database (Denmark)

    Ben Chaabane, Samir

    MicroRNAs (miRNAs) are small non-coding RNAs that function as guide molecules in RNA silencing. Plant miRNAs are critical for plant growth, development and stress response, and are processed in Arabidopsis from primary miRNA transcripts (pri-miRNAs) by the endonuclease activity of the DICER-LIKE1...... questions need to be addressed to establish a valid link, we provide encouraging evidence of the involvement of chromatin remodeling factors FAS1 and FAS2 in miRNA biogenesis. Together, we have expanded our understanding of the intersections between miRNA biogenesis and other pathways....

  16. Lawrence Berkeley Laboratory Institutional Plan FY 1987-1992

    Energy Technology Data Exchange (ETDEWEB)

    Various

    1986-12-01

    The Lawrence Berkeley Laboratory, operated by the University of California for the Department of Energy, provides national scientific leadership and supports technological innovation through its mission to: (1) Perform leading multidisciplinary research in general sciences and energy sciences; (2) Develop and operate unique national experimental facilities for use by qualified investigators; (3) Educate and train future generations of scientists and engineers; and (4) Foster productive relationships between LBL research programs and industry. The following areas of research excellence implement this mission and provide current focus for achieving DOE goals. GENERAL SCIENCES--(1) Accelerator and Fusion Research--accelerator design and operation, advanced accelerator technology development, accelerator and ion source research for heavy-ion fusion and magnetic fusion, and x-ray optics; (2) Nuclear Science--relativistic heavy-ion physics, medium- and low-energy nuclear physics, nuclear theory, nuclear astrophysics, nuclear chemistry, transuranium elements studies, nuclear data evaluation, and detector development; (3) Physics--experimental and theoretical particle physics, detector development, astrophysics, and applied mathematics. ENERGY SCIENCES--(1) Applied Science--building energy efficiency, solar for building systems, fossil energy conversion, energy storage, and atmospheric effects of combustion; (2) Biology and Medicine--molecular and cellular biology, diagnostic imaging, radiation biophysics, therapy and radiosurgery, mutagenesis and carcinogenesis, lipoproteins, cardiovascular disease, and hemopoiesis research; (3) Center for Advanced Materials--catalysts, electronic materials, ceramic and metal interfaces, polymer research, instrumentation, and metallic alloys; (4) Chemical Biodynamics--molecular biology of nucleic acids and proteins, genetics of photosynthesis, and photochemistry; (5) Earth Sciences--continental lithosphere properties, structures and

  17. miRConnect: Identifying Effector Genes of miRNAs and miRNA Families in Cancer Cells

    DEFF Research Database (Denmark)

    Hua, Youjia; Duan, Shiwei; Murmann, Andrea E

    2011-01-01

    have generated custom data sets containing expression information of 54 miRNA families sharing the same seed match. We have developed a novel strategy for correlating miRNAs with individual genes based on a summed Pearson Correlation Coefficient (sPCC) that mimics an in silico titration experiment......micro(mi)RNAs are small non-coding RNAs that negatively regulate expression of most mRNAs. They are powerful regulators of various differentiation stages, and the expression of genes that either negatively or positively correlate with expressed miRNAs is expected to hold information....... By focusing on the genes that correlate with the expression of miRNAs without necessarily being direct targets of miRNAs, we have clustered miRNAs into different functional groups. This has resulted in the identification of three novel miRNAs that are linked to the epithelial-to-mesenchymal transition (EMT...

  18. Circulating miRNAs miR-34a and miR-150 associated with colorectal cancer progression

    Czech Academy of Sciences Publication Activity Database

    Aherne, S.T.; Madden, S.F.; Hughes, D. J.; Pardini, B.; Naccarati, A.; Levý, M.; Vodička, Pavel; Neary, P.; Dowling, P.; Clynes, M.

    2015-01-01

    Roč. 15, apr 30 (2015), s. 2-13 ISSN 1471-2407 R&D Projects: GA ČR GAP304/10/1286 Institutional support: RVO:68378041 Keywords : colorectal cancer * circulating miRNAs * miR-34a * miR-150 * miR-923 Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 3.265, year: 2015

  19. Expression profiling of miR-96, miR-584 and miR-422a in colon ...

    African Journals Online (AJOL)

    . Lower miRNA ... Thus, the ratio of miR-96/miR-638 in plasma is a potential non- ... leading cause of cancer related deaths. ... breast cancer cells have revealed a total of 51 ... Corresponding negative control ..... The American Joint Committee.

  20. 34A, miRNA-944, miRNA-101 and miRNA-218 in cervical cancer

    African Journals Online (AJOL)

    RNAs (21 - 24 nucleotides in length) that are critical for many important processes such as development, ... RNA extraction and reverse transcription. Total RNA was extracted from each of the experimental groups using ... used as an endogenous control to normalize the expression of miRNA-143, miRNA-34A, miRNA-.

  1. Encuentro "Mi marca y yo"

    OpenAIRE

    Universidad de Alicante. Observatorio Comunicación en Cambio

    2013-01-01

    La gestión de la marca personal, especialmente en los entornos digitales, ha cobrado actualmente gran importancia como estrategia de posicionamiento profesional. Te invitamos a que asistas a nuestro encuentro "Mi marca y yo" para reflexionar sobre qué implica contar con una marca personal.

  2. Lawrence Livermore National Laboratory Surface Water Protection: A Watershed Approach

    Energy Technology Data Exchange (ETDEWEB)

    Coty, J

    2009-03-16

    This surface water protection plan (plan) provides an overview of the management efforts implemented at Lawrence Livermore National Laboratory (LLNL) that support a watershed approach to protect surface water. This plan fulfills a requirement in the Department of Energy (DOE) Order 450.1A to demonstrate a watershed approach for surface water protection that protects the environment and public health. This plan describes the use of a watershed approach within which the Laboratory's current surface water management and protections efforts have been structured and coordinated. With more than 800 million acres of land in the U.S. under federal management and stewardship, a unified approach across agencies provides enhanced resource protection and cost-effectiveness. The DOE adopted, along with other federal agencies, the Unified Federal Policy for a Watershed Approach to Federal Land and Resource Management (UFP) with a goal to protect water quality and aquatic ecosystems on federal lands. This policy intends to prevent and/or reduce water pollution from federal activities while fostering a cost-effective watershed approach to federal land and resource management. The UFP also intends to enhance the implementation of existing laws (e.g., the Clean Water Act [CWA] and National Environmental Policy Act [NEPA]) and regulations. In addition, this provides an opportunity for the federal government to serve as a model for water quality stewardship using a watershed approach for federal land and resource activities that potentially impact surface water and its uses. As a federal land manager, the Laboratory is responsible for a small but important part of those 800 million acres of land. Diverse land uses are required to support the Laboratory's mission and provide an appropriate work environment for its staff. The Laboratory comprises two sites: its main site in Livermore, California, and the Experimental Test Site (Site 300), near Tracy, California. The main site

  3. miRNAs in brain development

    International Nuclear Information System (INIS)

    Petri, Rebecca; Malmevik, Josephine; Fasching, Liana; Åkerblom, Malin; Jakobsson, Johan

    2014-01-01

    MicroRNAs (miRNAs) are small, non-coding RNAs that negatively regulate gene expression at the post-transcriptional level. In the brain, a large number of miRNAs are expressed and there is a growing body of evidence demonstrating that miRNAs are essential for brain development and neuronal function. Conditional knockout studies of the core components in the miRNA biogenesis pathway, such as Dicer and DGCR8, have demonstrated a crucial role for miRNAs during the development of the central nervous system. Furthermore, mice deleted for specific miRNAs and miRNA-clusters demonstrate diverse functional roles for different miRNAs during the development of different brain structures. miRNAs have been proposed to regulate cellular functions such as differentiation, proliferation and fate-determination of neural progenitors. In this review we summarise the findings from recent studies that highlight the importance of miRNAs in brain development with a focus on the mouse model. We also discuss the technical limitations of current miRNA studies that still limit our understanding of this family of non-coding RNAs and propose the use of novel and refined technologies that are needed in order to fully determine the impact of specific miRNAs in brain development. - Highlights: • miRNAs are essential for brain development and neuronal function. • KO of Dicer is embryonically lethal. • Conditional Dicer KO results in defective proliferation or increased apoptosis. • KO of individual miRNAs or miRNA families is necessary to determine function

  4. Potential role of miR-9 and miR-223 in recurrent ovarian cancer

    Directory of Open Access Journals (Sweden)

    McGuinness Eamonn

    2008-04-01

    Full Text Available Abstract Background MicroRNAs (miRNAs are small, noncoding RNAs that negatively regulate gene expression by binding to target mRNAs. miRNAs have not been comprehensively studied in recurrent ovarian cancer, yet an incurable disease. Results Using real-time RT-PCR, we obtained distinct miRNA expression profiles between primary and recurrent serous papillary ovarian adenocarcinomas (n = 6 in a subset of samples previously used in a transcriptome approach. Expression levels of top dysregulated miRNA genes, miR-223 and miR-9, were examined using TaqMan PCR in independent cohorts of fresh frozen (n = 18 and FFPE serous ovarian tumours (n = 22. Concordance was observed on TaqMan analysis for miR-223 and miR-9 between the training cohort and the independent test cohorts. Target prediction analysis for the above miRNA "recurrent metastatic signature" identified genes previously validated in our transcriptome study. Common biological pathways well characterised in ovarian cancer were shared by miR-9 and miR-223 lists of predicted target genes. We provide strong evidence that miR-9 acts as a putative tumour suppressor gene in recurrent ovarian cancer. Components of the miRNA processing machinery, such as Dicer and Drosha are not responsible for miRNA deregulation in recurrent ovarian cancer, as deluded by TaqMan and immunohistochemistry. Conclusion We propose a miRNA model for the molecular pathogenesis of recurrent ovarian cancer. Some of the differentially deregulated miRNAs identified correlate with our previous transcriptome findings. Based on integrated transcriptome and miRNA analysis, miR-9 and miR-223 can be of potential importance as biomarkers in recurrent ovarian cancer.

  5. Bioinformatics of cardiovascular miRNA biology.

    Science.gov (United States)

    Kunz, Meik; Xiao, Ke; Liang, Chunguang; Viereck, Janika; Pachel, Christina; Frantz, Stefan; Thum, Thomas; Dandekar, Thomas

    2015-12-01

    MicroRNAs (miRNAs) are small ~22 nucleotide non-coding RNAs and are highly conserved among species. Moreover, miRNAs regulate gene expression of a large number of genes associated with important biological functions and signaling pathways. Recently, several miRNAs have been found to be associated with cardiovascular diseases. Thus, investigating the complex regulatory effect of miRNAs may lead to a better understanding of their functional role in the heart. To achieve this, bioinformatics approaches have to be coupled with validation and screening experiments to understand the complex interactions of miRNAs with the genome. This will boost the subsequent development of diagnostic markers and our understanding of the physiological and therapeutic role of miRNAs in cardiac remodeling. In this review, we focus on and explain different bioinformatics strategies and algorithms for the identification and analysis of miRNAs and their regulatory elements to better understand cardiac miRNA biology. Starting with the biogenesis of miRNAs, we present approaches such as LocARNA and miRBase for combining sequence and structure analysis including phylogenetic comparisons as well as detailed analysis of RNA folding patterns, functional target prediction, signaling pathway as well as functional analysis. We also show how far bioinformatics helps to tackle the unprecedented level of complexity and systemic effects by miRNA, underlining the strong therapeutic potential of miRNA and miRNA target structures in cardiovascular disease. In addition, we discuss drawbacks and limitations of bioinformatics algorithms and the necessity of experimental approaches for miRNA target identification. This article is part of a Special Issue entitled 'Non-coding RNAs'. Copyright © 2014 Elsevier Ltd. All rights reserved.

  6. Measurement of target and double-spin asymmetries for the <mi>e><mi>pmi><mi>emi><mimi>+(<mi>n>) reaction in the nucleon resonance region at low <mi>Q>2

    Energy Technology Data Exchange (ETDEWEB)

    Zheng, X.; Adhikari, K. P.; Bosted, P.; Deur, A.; Drozdov, V.; El Fassi, L.; Kang, Hyekoo; Kovacs, K.; Kuhn, S.; Long, E.; Phillips, S. K.; Ripani, M.; Slifer, K.; Smith, L. C.; Adikaram, D.; Akbar, Z.; Amaryan, M. J.; Anefalos Pereira, S.; Asryan, G.; Avakian, H.; Badui, R. A.; Ball, J.; Baltzell, N. A.; Battaglieri, M.; Batourine, V.; Bedlinskiy, I.; Biselli, A. S.; Briscoe, W. J.; Bültmann, S.; Burkert, V. D.; Carman, D. S.; Celentano, A.; Chandavar, S.; Charles, G.; Chen, J. -P.; Chetry, T.; Choi, Seonho; Ciullo, G.; Clark, L.; Colaneri, L.; Cole, P. L.; Compton, N.; Contalbrigo, M.; Crede, V.; D' Angelo, A.; Dashyan, N.; De Vita, R.; De Sanctis, E.; Djalali, C.; Dodge, G. E.; Dupre, R.; Egiyan, H.; El Alaoui, A.; Elouadrhiri, L.; Eugenio, P.; Fanchini, E.; Fedotov, G.; Fersch, R.; Filippi, A.; Fleming, J. A.; Gevorgyan, N.; Ghandilyan, Y.; Gilfoyle, G. P.; Giovanetti, K. L.; Girod, F. X.; Gleason, C.; Golovach, E.; Gothe, R. W.; Griffioen, K. A.; Guidal, M.; Guler, N.; Guo, L.; Hanretty, C.; Harrison, N.; Hattawy, M.; Hicks, K.; Holtrop, M.; Hughes, S. M.; Ilieva, Y.; Ireland, D. G.; Ishkhanov, B. S.; Isupov, E. L.; Jenkins, D.; Jiang, H.; Jo, H. S.; Joosten, S.; Keller, D.; Khachatryan, G.; Khandaker, M.; Kim, A.; Kim, W.; Klein, F. J.; Kubarovsky, V.; Lanza, L.; Lenisa, P.; Livingston, K.; MacGregor, I. J. D.; Markov, N.; McKinnon, B.; Mirazita, M.; Mokeev, V.; Movsisyan, A.; Munevar, E.; Munoz Camacho, C.; Murdoch, G.; Nadel-Turonski, P.; Net, L. A.; Ni, A.; Niccolai, S.; Niculescu, G.; Niculescu, I.; Osipenko, M.; Ostrovidov, A. I.; Paolone, M.; Paremuzyan, R.; Park, K.; Pasyuk, E.; Peng, P.; Pisano, S.; Pogorelko, O.; Price, J. W.; Puckett, A. J. R.; Raue, B. A.; Rizzo, A.; Rosner, G.; Rossi, P.; Roy, P.; Sabatié, F.; Salgado, C.; Schumacher, R. A.; Sharabian, Y. G.; Skorodumina, Iu.; Smith, G. D.; Sokhan, D.; Sparveris, N.; Stankovic, I.; Strakovsky, I. I.; Strauch, S.; Taiuti, M.; Tian, Ye; Ungaro, M.; Voskanyan, H.; Voutier, E.; Walford, N. K.; Watts, D. P.; Wei, X.; Weinstein, L. B.; Wood, M. H.; Zachariou, N.; Zhang, J.; Zonta, I.

    2016-10-01

    We report measurements of target- and double-spin asymmetries for the exclusive channel <mi>e><mi>pmi><mi>emi><mimi>+(<mi>n>) in the nucleon resonance region at Jefferson Lab using the CEBAF Large Acceptance Spectrometer (CLAS). These asymmetries were extracted from data obtained using a longitudinally polarized NH3 target and a longitudinally polarized electron beam with energies 1.1, 1.3, 2.0, 2.3, and 3.0 GeV. The new results are consistent with previous CLAS publications but are extended to a low Q2 range from 0.0065 to 0.35 (GeV/c)2. The Q2 access was made possible by a custom-built Cherenkov detector that allowed the detection of electrons for scattering angles as low as 6 degrees. These results are compared with the unitary isobar models JANR and MAID, the partial-wave analysis prediction from SAID, and the dynamic model DMT. In many kinematic regions our results, in particular results on the target asymmetry, help to constrain the polarization-dependent components of these models.

  7. Perspectives on Sámi historiography

    Directory of Open Access Journals (Sweden)

    Lars Ivar Hansen

    2017-09-01

    Full Text Available The article focuses on Sámi history and historical methods. The main results and central aspects of Sámi history, in its relational context, are gone through. What effects and consequences — regarding both methodology and narrative styles — these aspects have had, and ought to have, for the processes of doing research on and writing Sámi history? The focus is on the politics of Sámi history and research. The issues, who is “allowed” to write Sámi history and the way Sámi research is demanded to stand in the service of different societal-cultural needs of the Sámi is dealt with. This expectation of applicability concerns Sámi history in general, and the more delimited efforts of presenting situated accounts of Sámi cultural practices, traditions and experience with relations to other folk groups. Finally, methodological considerations and recommendations of Sámi history are presented, in which a number of methodological competences and in-depth usage of numerous source categories are called for.

  8. 75 FR 10526 - In the Matter of Mr. Lawrence E. Grimm; Order Prohibiting Involvement in NRC-Licensed Activities

    Science.gov (United States)

    2010-03-08

    ... NUCLEAR REGULATORY COMMISSION [IA-09-068; NRC-2010-0085] In the Matter of Mr. Lawrence E. Grimm; Order Prohibiting Involvement in NRC-Licensed Activities I Mr. Lawrence E. Grimm was employed as a... NIST-Boulder facility in accordance with the conditions specified therein. Mr. Grimm was listed on the...

  9. Mr. Lawrence tuleb Rabarockiks kokku! Peer Günt uues kuues. Pervert ja ämma unistus

    Index Scriptorium Estoniae

    2007-01-01

    Rockansamblist Mr. Lawrence, albumitest "Mr. Lawrence", "Swing", "Sitandspin". Soome hardrock'i ansamblist Peer Günt, albumist "Backseat". Etno-folkrockansamblist Dagö (kontsert 15. juunil Rabarockil), albumitest "Toiduklubi", "Hiired tuules", "Joonistatud mees". Info festivalist: www.rabarock.delfi.ee / www.rabarock.ee

  10. Berkeley Lab's Saul Perlmutter wins E.O. Lawrence Award scientist's work on supernovae reveals accelerating universe

    CERN Multimedia

    2002-01-01

    "Saul Perlmutter, a member of Lawrence Berkeley National Laboratory's Physics Division and leader of the international Supernova Cosmology Project based there, has won the Department of Energy's 2002 E.O. Lawrence Award in the physics category" (1/2 page).

  11. miRiadne: a web tool for consistent integration of miRNA nomenclature.

    Science.gov (United States)

    Bonnal, Raoul J P; Rossi, Riccardo L; Carpi, Donatella; Ranzani, Valeria; Abrignani, Sergio; Pagani, Massimiliano

    2015-07-01

    The miRBase is the official miRNA repository which keeps the annotation updated on newly discovered miRNAs: it is also used as a reference for the design of miRNA profiling platforms. Nomenclature ambiguities generated by loosely updated platforms and design errors lead to incompatibilities among platforms, even from the same vendor. Published miRNA lists are thus generated with different profiling platforms that refer to diverse and not updated annotations. This greatly compromises searches, comparisons and analyses that rely on miRNA names only without taking into account the mature sequences, which is particularly critic when such analyses are carried over automatically. In this paper we introduce miRiadne, a web tool to harmonize miRNA nomenclature, which takes into account the original miRBase versions from 10 up to 21, and annotations of 40 common profiling platforms from nine brands that we manually curated. miRiadne uses the miRNA mature sequence to link miRBase versions and/or platforms to prevent nomenclature ambiguities. miRiadne was designed to simplify and support biologists and bioinformaticians in re-annotating their own miRNA lists and/or data sets. As Ariadne helped Theseus in escaping the mythological maze, miRiadne will help the miRNA researcher in escaping the nomenclature maze. miRiadne is freely accessible from the URL http://www.miriadne.org. © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

  12. Analytical capabilities and services of Lawrence Livermore Laboratory's General Chemistry Division

    International Nuclear Information System (INIS)

    Gutmacher, R.; Crawford, R.

    1978-01-01

    This comprehensive guide to the analytical capabilities of Lawrence Livermore Laboratory's General Chemistry Division describes each analytical method in terms of its principle, field of application, and qualitative and quantitative uses. Also described are the state and quantity of sample required for analysis, processing time, available instrumentation, and responsible personnel

  13. Lawrence Livermore National Laboratory DIII-D cooperation: 1987 annual report

    International Nuclear Information System (INIS)

    Allen, S.L.; Calderon, M.O.; Ellis, R.M.

    1988-01-01

    This report summarizes the Lawrence Livermore National Laboratory (LLNL) DIII-D cooperation during FY87. The LLNL participation in DIII-D concentrated on three principal areas: ECH and current-drive physics, divertor and edge physics, and tokamak operations. These topics are dicussed in this report. 27 refs., 11 figs

  14. Baking the Bread and Roasting the Meat : Dorlandus's Saint Lawrence as a model for Carthusians

    NARCIS (Netherlands)

    van Dijk, Mathilde

    2018-01-01

    The two sermons on Saint Lawrence by the profilic Carthusian author Petrus Dorlandus survive in a manuscript from the charterhouse in Louvain, which was used for reading at table. Dorlandus’s development of the well-known tale about this martyr is geared to this practice. Inviting his fellow

  15. Lawrence Livermore Laboratory's beryllium control program for high-explosive test firing bunkers and tables

    International Nuclear Information System (INIS)

    Johnson, J.S.

    1978-01-01

    This detailed report on Lawrence Livermore Laboratory's control program to minimize beryllium levels in Laboratory workplaces includes an outline of beryllium surface, soil, and air levels and an 11-y summary of sampling results from two high-use, high-explosive test firing bunkers. These sampling data and other studies demonstrate that the beryllium control program is funcioning effectively

  16. Final Report Bald and Golden Eagle Territory Surveys for the Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Fratanduono, M. L. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2014-11-25

    Garcia and Associates (GANDA) was contracted by the Lawrence Livermore National Laboratory (LLNL) to conduct surveys for bald eagles (Haliaeetus leucocephalus) and golden eagles (Aquila chrysaetos) at Site 300 and in the surrounding area out to 10-miles. The survey effort was intended to document the boundaries of eagle territories by careful observation of eagle behavior from selected viewing locations throughout the study area.

  17. High-pressure safety at the Lawrence Livermore Laboratory, an energy research facility

    International Nuclear Information System (INIS)

    Burton, W.A.

    1976-01-01

    The high-pressure safety program at Lawrence Livermore Laboratory, Livermore, California, has been successful in preventing lost-time high-pressure accidents over the past 12 years. Program organization, personnel training and qualification, pressure vessel design criteria and documentation, and pressure testing and inspection are discussed

  18. Attraction of Diabrotica barberi Smith and Lawrence (Coleoptera: Chrysomelidae) to eugenol-baited traps in soybean

    Science.gov (United States)

    Diabrotica barberi Smith and Lawrence (the northern corn rootworm) is a native North American leaf beetle and a major pest of corn. However, adult D. barberi forage in various habitats outside of corn, including soybean, roadside vegetation, and prairie. Eugenol is a common floral volatile that ha...

  19. the contribution made by te lawrence to the theory of revolutionary

    African Journals Online (AJOL)

    A short resume of T.E. Lawrence's early life and his role in World War I ... intelligence section in Cairo.3) The Arabs in the. Hejezarea ... time and artificial aids were taken into account. In short, anything ... was considered to be the humanity in battle, the material could .... His leadership and the quality thereof comes to the fore ...

  20. The Procedural Queer: Substantive Due Process, "Lawrence v. Texas," and Queer Rhetorical Futures

    Science.gov (United States)

    Campbell, Peter Odell

    2012-01-01

    This essay discusses Justice Anthony M. Kennedy's choice to foreground arguments from due process rather than equal protection in the majority opinion in Lawrence v. Texas. Kennedy's choice can realize constitutional legal doctrine that is more consistent with radical queer politics than arguments from equal protection. Unlike some recent…

  1. A Constructive Replication of the Lawrence and Lorsch Conflict Resolution Methodology.

    Science.gov (United States)

    Fry, Louis W.; And Others

    1980-01-01

    A replication of Lawrence and Lorsch's (1967) findings of three modes of conflict resolution did not yield a clear factor structure. The validity of the scale for purposes of measuring conflict resolution modes is seriously questioned as is what is taught in the area of conflict resolution. (Author)

  2. Tolerance, Acceptance and the Virtue of Orthonomy: A Reply to Lawrence Blum and Brenda Almond

    Science.gov (United States)

    Ciurria, Michelle

    2011-01-01

    In the "Journal of Moral Education," 39(2), Brenda Almond and Lawrence Blum debate the importance of tolerance versus acceptance in sex education. Blum defines acceptance as "positive regard", in contradistinction to mere tolerance, "a live and let live attitude toward others, an acceptance of coexistence, but with a…

  3. Moral Maturity and Autonomy: Appreciating the Significance of Lawrence Kolhberg's Just Community

    Science.gov (United States)

    McDonough, Graham P.

    2005-01-01

    Lawrence Kohlberg's Just Community program of moral education has conceptual significance to his theoretical work in the field of moral development. This argument contends that a perspective recognizing the Just Community as conceptually significant provides a more comprehensive picture of Kohlberg's work than do critical perspectives that limit…

  4. Remedial investigation and feasibility study for the Lawrence Livermore National Laboratory Site 300 Pit 7 Complex

    Energy Technology Data Exchange (ETDEWEB)

    Taffet, M.J. (Lawrence Livermore National Lab., CA (USA)); Oberdorfer, J.A. (San Jose State Univ., CA (USA)); McIlvride, W.A. (Weiss Associates, Oakland, CA (USA))

    1989-10-01

    This report summarizes the results and conclusions of the investigation of tritium and other compounds in ground water in the vicinity of landfills at the Lawrence Livermore National Laboratory (LLNL) Site 300 Pit 7 Complex. 91 refs., 110 figs., 43 tabs.

  5. Lawrence Livermore National Laboratory selects Intel Itanium 2 processors for world's most powerful Linux cluster

    CERN Multimedia

    2003-01-01

    "Intel Corporation, system manufacturer California Digital and the University of California at Lawrence Livermore National Laboratory (LLNL) today announced they are building one of the world's most powerful supercomputers. The supercomputer project, codenamed "Thunder," uses nearly 4,000 Intel® Itanium® 2 processors... is expected to be complete in January 2004" (1 page).

  6. A Revolution in the Education of Women. Ten Years of Continuing Education at Sarah Lawrence College.

    Science.gov (United States)

    Richter, Melissa Lewis; Whipple, Jane Banks

    The Sarah Lawrence Continuing Education Center provides educational opportunities for women who are not now in college, but who wish to continue their educations. This book is a publication about and a catalog for the Continuing Education Center. The Undergraduate Program is explained, as are procedures for admission, financial aid, course work,…

  7. Health assessment for Brodhead Creek, Stroudsburg, Monroe County, Pennsylvania, Region 3. CERCLIS No. PAD98091760. Addendum, December 27, 1991. Preliminary report

    International Nuclear Information System (INIS)

    1991-01-01

    The Brodhead Creek Site is listed on the Environmental Protection Agency's National Priorities List (NPL). The 12-acre site, located in Stroudsburg, Monroe County, Pennsylvania, is adjacent to two creeks between the Route 209 bridge and the Route 80 bridge. Coal tar waste from a coal gasification plant was disposed of on site into two large lagoons and later covered with fill. The plant was dismantled in 1948. The Remedial Investigation (RI) of the site was performed in 1988-89 and revealed coal tar compounds (arsenic, benzene, cyanide, and polynuclear aromatic hydrocarbons) as the chemicals of concern. The site is judged to be an indeterminate public health hazard because the available data does not indicate humans are being or have been exposed to levels of contamination that would be expected to cause adverse health effects. A potential public health threat exists for chronic exposure to creek biota via ingestion, to the creek sediment from direct exposure, and to workers during any remediation which may allow for pathways of exposure to occur from groundwater and soil contact or inhalation. Future land use may also create potential routes of exposure. Although vertical migration appears to be limited by the sand unit, the subsurface coal tar pools onsite may serve as a potential source of release to the deep bedrock aquifer

  8. The miRNome of bipolar disorder.

    Science.gov (United States)

    Fries, Gabriel R; Carvalho, Andre F; Quevedo, Joao

    2018-06-01

    Epigenetic mechanisms have been suggested to play a key role in the pathophysiology of bipolar disorder (BD), among which microRNAs (miRNAs) may be of particular significance according to recent studies. We aimed to summarize miRNA studies in BD to identify consistent findings, limitations, and future directions of this emerging field. We performed a comprehensive search on PUBMED and Medline for studies investigating an association between BD and miRNAs. The included studies report miRNA alterations in postmortem brain tissues and in the periphery, cell culture and preclinical findings, genetic associations, and the effects of medications. Several studies report changes in miRNA expression levels in postmortem brain and in the periphery of patients, although most of the results so far have not been replicated and are not concordant between different populations. Genetic studies also suggest that miRNA genes are located within susceptibility loci of BD, and also a putative role of miRNAs in modulating genes previously shown to confer risk of BD. We did not perform a systematic review of the literature, and miRNAs represent only one facet of the plethora of epigenetic mechanisms that might be involved in BD's pathophysiology. miRNA findings in BD significantly vary between studies, but are consistent to suggest a key role for these molecules in BD's pathophysiology and treatment, particularly miR-34a and miR-137. Accordingly, miRNA might represent important biomarkers of illness to be used in the clinical settings, and potentially also for the development of novel therapeutics for BD in the near future. Copyright © 2017 Elsevier B.V. All rights reserved.

  9. About miRNAs, miRNA seeds, target genes and target pathways.

    Science.gov (United States)

    Kehl, Tim; Backes, Christina; Kern, Fabian; Fehlmann, Tobias; Ludwig, Nicole; Meese, Eckart; Lenhof, Hans-Peter; Keller, Andreas

    2017-12-05

    miRNAs are typically repressing gene expression by binding to the 3' UTR, leading to degradation of the mRNA. This process is dominated by the eight-base seed region of the miRNA. Further, miRNAs are known not only to target genes but also to target significant parts of pathways. A logical line of thoughts is: miRNAs with similar (seed) sequence target similar sets of genes and thus similar sets of pathways. By calculating similarity scores for all 3.25 million pairs of 2,550 human miRNAs, we found that this pattern frequently holds, while we also observed exceptions. Respective results were obtained for both, predicted target genes as well as experimentally validated targets. We note that miRNAs target gene set similarity follows a bimodal distribution, pointing at a set of 282 miRNAs that seems to target genes with very high specificity. Further, we discuss miRNAs with different (seed) sequences that nonetheless regulate similar gene sets or pathways. Most intriguingly, we found miRNA pairs that regulate different gene sets but similar pathways such as miR-6886-5p and miR-3529-5p. These are jointly targeting different parts of the MAPK signaling cascade. The main goal of this study is to provide a general overview on the results, to highlight a selection of relevant results on miRNAs, miRNA seeds, target genes and target pathways and to raise awareness for artifacts in respective comparisons. The full set of information that allows to infer detailed results on each miRNA has been included in miRPathDB, the miRNA target pathway database (https://mpd.bioinf.uni-sb.de).

  10. Diagnostic potential of miR-126, miR-143, miR-145, and miR-652 in malignant pleural mesothelioma

    DEFF Research Database (Denmark)

    Andersen, Morten; Grauslund, Morten; Ravn, Jesper

    2014-01-01

    Malignant pleural mesothelioma (MPM) is difficult to distinguish from reactive mesothelial proliferations (RMPs). It is uncertain whether miRNAs are useful biomarkers for differentiating MPM from RMPs. Thus, we screened with a quantitative RT-PCR (RT-qPCR)-based platform the expression of 742 miR...

  11. Taking a multiple intelligences (MI) perspective.

    Science.gov (United States)

    Gardner, Howard

    2017-01-01

    The theory of multiple intelligences (MI) seeks to describe and encompass the range of human cognitive capacities. In challenging the concept of general intelligence, we can apply an MI perspective that may provide a more useful approach to cognitive differences within and across species.

  12. Miércoles al cine

    OpenAIRE

    Aguado Franco, Juan Carlos

    2014-01-01

    Se analiza un caso concreto de demanda ante una iniciativa empresarial: los miércoles al cine. Se analiza un caso concreto de demanda ante una iniciativa empresarial: los miércoles al cine. Fundamentos del Análisis Económico

  13. miRNAFold: a web server for fast miRNA precursor prediction in genomes.

    Science.gov (United States)

    Tav, Christophe; Tempel, Sébastien; Poligny, Laurent; Tahi, Fariza

    2016-07-08

    Computational methods are required for prediction of non-coding RNAs (ncRNAs), which are involved in many biological processes, especially at post-transcriptional level. Among these ncRNAs, miRNAs have been largely studied and biologists need efficient and fast tools for their identification. In particular, ab initio methods are usually required when predicting novel miRNAs. Here we present a web server dedicated for miRNA precursors identification at a large scale in genomes. It is based on an algorithm called miRNAFold that allows predicting miRNA hairpin structures quickly with high sensitivity. miRNAFold is implemented as a web server with an intuitive and user-friendly interface, as well as a standalone version. The web server is freely available at: http://EvryRNA.ibisc.univ-evry.fr/miRNAFold. © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

  14. The health protection of riparian populations in case of a major oil spill:. The St. Lawrence Vision 2000 Shores project

    International Nuclear Information System (INIS)

    Guerrier, P.; Tardif, G.

    1995-01-01

    Objectives of a research project to protect the health of riparian populations in the event of a major oil spill in the St. Lawrence river were summarized. The project is a part of the health component of the St. Lawrence Vision 2000 (SLV-2000) action plan, which is a product of co-operation among Environment Canada, Health Canada, le ministere de la Sante et des Services sociaux du Quebec, and other government departments, designed to protect and conserve the St. Lawrence river and its environment

  15. Ernest Orlando Lawrence Awards Ceremony for 2011 Award Winners (Presentations, including remarks by Energy Secretary, Dr. Steven Chu)

    International Nuclear Information System (INIS)

    Chu, Steven

    2012-01-01

    The winners for 2011 of the Department of Energy's Ernest Orlando Lawrence Award were recognized in a ceremony held May 21, 2012. Dr. Steven Chu and others spoke of the importance of the accomplishments and the prestigious history of the award. The recipients of the Ernest Orlando Lawrence Award for 2011 are: Riccardo Betti (University of Rochester); Paul C. Canfield (Ames Laboratory); Mark B. Chadwick (Los Alamos National Laboratory); David E. Chavez (Los Alamos National Laboratory); Amit Goyal (Oak Ridge National Laboratory); Thomas P. Guilderson (Lawrence Livermore National Laboratory); Lois Curfman McInnes (Argonne National Laboratory); Bernard Matthew Poelker (Thomas Jeffereson National Accelerator Facility); and Barry F. Smith (Argonne National Laboratory).

  16. Mi oíslo

    Directory of Open Access Journals (Sweden)

    Mitja Skubic

    2006-12-01

    Full Text Available Hablando Sancho en su primer encuentro con don Quijote de su mujer Juana Gutiérrez, llamada en seguida Teresa Panza o simplemente Teresa, la nombra mi oíslo y repite lo mismo otras dos veces. Conviene tener presente que de su mujer Sancho habla siempre respetuosamente y con mucho cariño, con excepción de una conversación que llega a ser un litigio, II, 5. Allí, Sancho expresa su idea de cómo y con quién casar a la hija Sanchica y por fin impone su voluntad. Teresa se le opone vigorosamente y esto induce a Sancho, sobreexcitado, a formular una gradación sorprendente: mujer mía; mirad, Teresa; mujer; calla, boba; bestia y mujer de Barrabás; animalia; mentecata e ignorante.

  17. Bi-national Great Lakes-St. Lawrence Basin climate change and hydrologic scenarios report

    Energy Technology Data Exchange (ETDEWEB)

    Lavender, B.; Smith, J.V.; Koshida, G.; Mortsch, L.D. [eds.

    1998-09-01

    Climate experts in government, industry and academic institutions have put together a national assessment of how climate change will affect Canadians and their social, biological and economic environment over the next century. This volume documents the impacts and implications of climate change on the Great Lakes-St. Lawrence Basin, and provides an analysis and assessment of various climate and hydrologic scenarios used for the Great Lakes - St. Lawrence Basin Project. As part of the analysis and assessment, results from the Canadian Climate Centre second-generation General Circulation Model and four transposition scenarios for both climate and hydrological resources are reviewed. The objective is to provide an indication of sensitivities and vulnerabilities of the region to climate, with a view to improve adaptation to potential climate changes. 25 tabs., 26 figs. figs.

  18. Cleaning priority index for aquatic birds vulnerable to oil spills in the St. Lawrence

    International Nuclear Information System (INIS)

    Daigle, S.; Darveau, M.

    1995-01-01

    A cleaning priority index for 120 species of aquatic birds present in the St. Lawrence River system and vulnerable to the effects of oil spills, was developed. Six criteria were considered in drawing up the index: (1) world population size, (2) productivity, (3) vulnerability, (4) local numbers, (5) economic value, and (6) survival during rehabilitation. Birds were assessed against each of the criteria, using a semi-quantitative ordinal scale ranging from 0 to 5. The index can be used as a decision-making tool for directing oiled-bird capture and cleaning. In the case of individual birds, the physiological condition of the bird in question should be used as an additional point of reference when deciding which bird to clean. Use of the index is not confined to the St. Lawrence River; it is readily adaptable to any other region. 58 refs., 4 tabs

  19. Clinical results of stereotactic hellium-ion radiosurgery of the pituitary gland at Lawrence Berkeley Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Levy, R.P.; Fabrikant, J.I.; Lyman, J.T.; Frankel, K.A.; Phillips, M.H.; Lawrence, J.H.; Tobias, C.A.

    1989-12-01

    The first therapeutic clinical trial using accelerated heavy-charged particles in humans was performed at Lawrence Berkeley Laboratory (LBL) for the treatment of various endocrine and metabolic disorders of the pituitary gland, and as suppressive therapy for adenohypophyseal hormone-responsive carcinomas and diabetic retinopathy. In acromegaly, Cushing's disease, Nelson's syndrome and prolactin-secreting tumors, the therapeutic goal in the 433 patients treated has been to destroy or inhibit the growth of the pituitary tumor and control hormonal hypersecretion, while preserving a functional rim of tissue with normal hormone-secreting capacity, and minimizing neurologic injury. An additional group of 34 patients was treated for nonsecreting chromophobe adenomas. This paper discusses the methods and results of stereotactic helium-ion radiosurgery of the pituitary gland at Lawrence Berkeley Laboratory. 11 refs.

  20. Comparison of fishes in nearshore areas of the St. Lawrence River, New York over 35 years

    Science.gov (United States)

    Carlson, Douglas M.; McKenna, James E.

    2014-01-01

    Fishes of the nearshore waters of the St. Lawrence River provide forage for valuable sport fisheries and are important biological indicators of condition and change. This fish community differs slightly among various reaches of the St. Lawrence River from New York to Quebec (Carlson et al. 2006, Eckert and Hanlon 1977, Kapuscinski 2011, LaViolette et al. 2003, Mandrak et al. 2006, McKenna et al. 2005). Nearshore habitat has been described by McKenna et al. (2012), and others have suggested that there were changes over the last few decades (Clapsadl 1993, Kapuscinski and Farrell 2013). More definitive work needs to be completed on submerged aquatic vegetation habitats. In this paper, changes in the nearshore fish species composition for the New York reach from Cape Vincent to Moses-Saunders Dam are examined through comparison of results from 2009-2010 (McKenna et al. 2012) and 1976 surveys (Eckert and Hanlon 1977).

  1. Clinical results of stereotactic hellium-ion radiosurgery of the pituitary gland at Lawrence Berkeley Laboratory

    International Nuclear Information System (INIS)

    Levy, R.P.; Fabrikant, J.I.; Lyman, J.T.; Frankel, K.A.; Phillips, M.H.; Lawrence, J.H.; Tobias, C.A.

    1989-12-01

    The first therapeutic clinical trial using accelerated heavy-charged particles in humans was performed at Lawrence Berkeley Laboratory (LBL) for the treatment of various endocrine and metabolic disorders of the pituitary gland, and as suppressive therapy for adenohypophyseal hormone-responsive carcinomas and diabetic retinopathy. In acromegaly, Cushing's disease, Nelson's syndrome and prolactin-secreting tumors, the therapeutic goal in the 433 patients treated has been to destroy or inhibit the growth of the pituitary tumor and control hormonal hypersecretion, while preserving a functional rim of tissue with normal hormone-secreting capacity, and minimizing neurologic injury. An additional group of 34 patients was treated for nonsecreting chromophobe adenomas. This paper discusses the methods and results of stereotactic helium-ion radiosurgery of the pituitary gland at Lawrence Berkeley Laboratory. 11 refs

  2. Mobility of Tritium in Engineered and Earth Materials at the NuMI Facility, Fermilab: Progress report for work performed between June 13 and September 30, 2006

    International Nuclear Information System (INIS)

    Pruess, Karsten; Conrad, Mark; Finsterle, Stefan; Kennedy, Mack; Kneafsey, Timothy; Salve, Rohit; Su, Grace; Zhou, Quanlin

    2006-01-01

    This report details the work done between June 13 and September 30, 2006 by Lawrence Berkeley National Laboratory (LBNL) scientists to assist Fermi National Accelerator Laboratory (Fermilab) staff in understanding tritium transport at the Neutrino at the Main Injector (NuMI) facility. As a byproduct of beamline operation, the facility produces (among other components) tritium in engineered materials and the surrounding rock formation. Once the tritium is generated, it may be contained at the source location, migrate to other regions within the facility, or be released to the environment

  3. Radioactive and mixed waste management plan for the Lawrence Berkeley Laboratory Hazardous Waste Handling Facility

    International Nuclear Information System (INIS)

    1995-01-01

    This Radioactive and Mixed Waste Management Plan for the Hazardous Waste Handling Facility at Lawrence Berkeley Laboratory is written to meet the requirements for an annual report of radioactive and mixed waste management activities outlined in DOE Order 5820.2A. Radioactive and mixed waste management activities during FY 1994 listed here include principal regulatory and environmental issues and the degree to which planned activities were accomplished

  4. The value of assessments in Lawrence Livermore National Laboratory's Waste Certification Programs

    International Nuclear Information System (INIS)

    Ryan, E.M.

    1995-05-01

    This paper will discuss the value of assessments in Lawrence Livermore National Laboratory's Waste Certification Programs by: introducing the organization and purpose of the LLNL Waste Certification Programs for transuranic, low-level, and hazardous waste; examining the differences in internal assessment/audit requirements for these programs; discussing the values and costs of assessments in a waste certification program; presenting practical recommendations to maximize the value of your assessment programs; and presenting improvements in LLNL's waste certification processes that resulted from assessments

  5. 2003 Lawrence Livermore National Laboratory Annual Illness and Injury Surveillance Report

    Energy Technology Data Exchange (ETDEWEB)

    U.S. Department of Energy, Office of Health, Safety and Security, Office of Illness and Injury Prevention Programs

    2007-05-23

    Annual Illness and Injury Surveillance Program report for 2003 for Lawrence Livermore National Lab. The U.S. Department of Energy’s (DOE) commitment to assuring the health and safety of its workers includes the conduct of epidemiologic surveillance activities that provide an early warning system for health problems among workers. The IISP monitors illnesses and health conditions that result in an absence of workdays, occupational injuries and illnesses, and disabilities and deaths among current workers.

  6. Nuclear physics and heavy element research at Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Stoyer, Mark A; Ahle, L E; Becker, J A; Bernshein, L A; Bleuel, D L; Burke, J T; Dashdorj, D; Henderson, R A; Hurst, A M; Kenneally, Jacqueline M; Lesher, S R; Moody, K J; Nelson, S L; Norman, E B; Pedretti, M; Scielzo, N D; Shaughnessy, D A; Sheets, S A; Stoeffl, W; Stoyer, N J [Lawrence Livermore National Laboratory, University of California, Livermore (United States)

    2009-12-31

    This paper highlights some of the current basic nuclear physics research at Lawrence Livermore National Laboratory (LLNL). The work at LLNL concentrates on investigating nuclei at the extremes. The Experimental Nuclear Physics Group performs research to improve our understanding of nuclei, nuclear reactions, nuclear decay processes and nuclear astrophysics; an expertise utilized for important laboratory national security programs and for world-class peer-reviewed basic research.

  7. The Oligochaeta (Annelida, Clitellata) of the St. Lawrence Great Lakes region: An update

    Science.gov (United States)

    Spencer, Douglas R.; Hudson, Patrick L.

    2003-01-01

    An updated oligochaete species list for the Great Lakes region is provided. The list was developed through the reexamination of the taxa reported in a previous report in 1980, addition of new taxa or records collected from the region since 1980, and an update of taxonomy commensurate with systematic and nomenclatural changes over the intervening years since the last review. The authors found 74 papers mentioning Great Lakes oligochaete species. The majority of these papers were published in the 1980s. The literature review and additional collections resulted in 15 species being added to the previous list. Nine taxa were removed from the previous list due to misidentification, synonymies, level of identification, or inability to confirm the identity. Based on this review, 101 species of Oligochaeta are now known from the St. Lawrence Great Lakes watershed. Of these, 95 species are known from the St. Lawrence Great Lakes proper, with an additional 6 species recorded from the inland waters of the watershed. The greatest diversity of oligochaete species was found in the inland waters of the region (81) followed by Lake Huron (72), Lake Ontario (65), Lake Erie (64), Lake Superior (63), Lake Michigan (62), St. Marys River (60), Niagara River (49), Saginaw Bay (44), St. Clair River (37), Lake St. Clair (36), St. Lawrence River (27), and the Detroit River (21). Three species are suspected of being introduced, Branchiura sowerbyi, Gianius aquaedulcisand Ripistes parasita, and two are believed to be endemic, Thalassodrilus hallae andTeneridrilus flexus.

  8. Indication of a Lombard vocal response in the St. Lawrence River beluga

    Science.gov (United States)

    Scheifele, P. M.; Andrew, S.; Cooper, R. A.; Darre, M.; Musiek, F. E.; Max, L.

    2005-03-01

    Noise pollution is recognized as a potential danger to marine mammals in general, and to the St. Lawrence beluga in particular. One method of determining the impacts of noise on an animal's communication is to observe a natural and repeatable response of the vocal system to variations in noise level. This is accomplished by observing intensity changes in animal vocalizations in response to environmental noise. One such response observed in humans, songbirds, and some primates is the Lombard vocal response. This response represents a vocal system reaction manifested by changes in vocalization level in direct response to changes in the noise field. In this research, a population of belugas in the St. Lawrence River Estuary was tested to determine whether a Lombard response existed by using hidden Markhov-classified vocalizations as targets for acoustical analyses. Correlation and regression analyses of signals and noise indicated that the phenomenon does exist. Further, results of human subjects experiments [Egan, J. J. (1966), Ph.D. dissertation; Scheifele, P. M. (2003), Ph.D. dissertation], along with previously reported data from other animal species, are similar to those exhibited by the belugas. Overall, findings suggest that typical noise levels in the St. Lawrence River Estuary have a detectable effect on the communication of the beluga. .

  9. Atlas of ecologically and commercially important areas in the southern Gulf of St. Lawrence

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-10-01

    This atlas provides useful baseline information for the environmental assessment of petroleum project proposals relative to sensitive areas and critical periods of fisheries resources in the southern Gulf of St. Lawrence. The southern boundary of the Gulf of St. Lawrence is bounded by the shores of western Cape Breton, southeastern New Brunswick and northern Nova Scotia. It is characterized as a coastal marine ecosystem because of the predominating effects of the rivers, but deeper areas have more in common with the Scotian Shelf and Slope and many common species move seasonally between the Gulf and the open Atlantic Ocean. This report is intended mostly for use by the Canada-Nova Scotia Offshore Petroleum Board (C-NSOPB), and to a lesser degree the Canada-Newfoundland Offshore Petroleum Board. The maps illustrate the known distribution of certain ecosystem components and known harvesting areas. The first part of the report describes the environmental setting of the southern Gulf of St. Lawrence. The report describes commercially important resources such as benthic, pelagic and shellfish species and other minor species, as well as aquaculture leases. It also discuses seals, whales, raptors, shorebirds and coastal waterfowl. The report identifies tourist areas, recreational parks and protected areas. Seasonality and sensitive timing of the resources are also summarized. refs., tabs., figs.

  10. Metal contamination in zebra mussels (Dreissena polymorpha) along the St. Lawrence River.

    Science.gov (United States)

    Kwan, K H Michael; Chan, Hing Man; de Lafontaine, Yves

    2003-01-01

    In order to evaluate the use of zebra mussels as biomonitors for metal bioavailability in the St. Lawrence River, we tested the hypothesis that the concentrations of 11 metals in zebra mussels vary significantly between sites along the river and that the season of collection and body size affect metal bioaccumulation. Mussels were collected at 14 sites during June 1996 and at monthly intervals at one site. Specimens were grouped in three size classes and their soft tissue was analyzed for As, Ca, Cd, Cr, Hg, Mn, Ni, Pb, Se, and Zn. Significant size effects were found for Ca, Cd, Cr, Cu, Ni and Zn. Spatial and seasonal variations in bioconcentration were significant for all metals. Spatial patterns in contamination that corresponded to known point sources of pollution or hydrology of the river were identified by principal component analysis. Seasonal variations can be attributed to the reproductive cycle of mussels and hydrological variability of the river. In comparison with values reported for zebra mussels in other contaminated sites in North America and Europe, levels of metal in the St. Lawrence River are low or intermediate. Our results show that when controlled for size and seasonal effects, zebra mussels represent a useful biomonitor for metal availability in the river and may offer an interesting alternative to native mussels and fish for such a role. Local contamination by some toxic metals is still a cause for concern in the St. Lawrence River.

  11. Paul Monroe e a circulação de uma modalidade de narrativa para se pensar e ensinar as histórias da educação

    OpenAIRE

    José Cláudio Sooma Silva; Marta Regina Gimenez Favaro

    2014-01-01

    O artigo estabelece interlocuções com as reflexões de outros pesquisadores que, igualmente, elegeram a obra norte- americana A brief course in the history of education, elaborada por Paul Monroe em 1907, como objeto de estudo e a sua escrita como foco de investigação. Nessa medida, problematiza o enfoque enciclopédico adotado, os realces conferidos e os silenciamentos produzidos em relação às experiências educacionais orquestradas no passado. A significativa circulação...

  12. Altering β-cell number through stable alteration of miR-21 and miR-34a expression

    DEFF Research Database (Denmark)

    Backe, Marie Balslev; Novotny, Guy Wayne; Christensen, Dan Ploug

    2014-01-01

    RNAs, miR-21 and miR-34a, may be involved in mediating cytokine-induced β-cell dysfunction. Therefore, manipulation of miR-21 and miR-34a levels may potentially be beneficial to β cells. To study the effect of long-term alterations of miR-21 or miR-34a levels upon net β-cell number, we stably overexpressed...

  13. MiMiR: a comprehensive solution for storage, annotation and exchange of microarray data

    Directory of Open Access Journals (Sweden)

    Rahman Fatimah

    2005-11-01

    Full Text Available Abstract Background The generation of large amounts of microarray data presents challenges for data collection, annotation, exchange and analysis. Although there are now widely accepted formats, minimum standards for data content and ontologies for microarray data, only a few groups are using them together to build and populate large-scale databases. Structured environments for data management are crucial for making full use of these data. Description The MiMiR database provides a comprehensive infrastructure for microarray data annotation, storage and exchange and is based on the MAGE format. MiMiR is MIAME-supportive, customised for use with data generated on the Affymetrix platform and includes a tool for data annotation using ontologies. Detailed information on the experiment, methods, reagents and signal intensity data can be captured in a systematic format. Reports screens permit the user to query the database, to view annotation on individual experiments and provide summary statistics. MiMiR has tools for automatic upload of the data from the microarray scanner and export to databases using MAGE-ML. Conclusion MiMiR facilitates microarray data management, annotation and exchange, in line with international guidelines. The database is valuable for underpinning research activities and promotes a systematic approach to data handling. Copies of MiMiR are freely available to academic groups under licence.

  14. Identification of miR-93 as a suitable miR for normalizing miRNA in plasma of tuberculosis patients.

    Science.gov (United States)

    Barry, Simone E; Chan, Brian; Ellis, Magda; Yang, YuRong; Plit, Marshall L; Guan, Guangyu; Wang, Xiaolin; Britton, Warwick J; Saunders, Bernadette M

    2015-07-01

    Tuberculosis (TB) remains a major public health issue. New tests to aid diagnoses and monitor the response to therapy are urgently required. There is growing interest in the use of microRNA (miRNA) profiles as diagnostic, prognostic or predictive markers in a range of clinical and infectious diseases, including Mycobacterium tuberculosis infection, however, challenges exist to accurately normalise miRNA levels in cohorts. This study examined the appropriateness of 12 miRs and RNU6B to normalise circulating plasma miRNA levels in individuals with active TB from 2 different geographical and ethnic regions. Twelve miRs (let-7, miR-16, miR-22, miR-26, miR-93, miR-103, miR-191, miR-192, miR-221, miR-423, miR-425 and miR-451) and RNU6B were selected based on their reported production by lung cells, expression in blood and previous use as a reference miRNA. Expression levels were analysed in the plasma of newly diagnosed TB patients from Australia and China compared with individuals with latent TB infection and healthy volunteers. Analysis with both geNorm and NormFinder software identified miR-93 as the most suitable reference miR in both cohorts, either when analysed separately or collectively. Interestingly, there were large variations in the expression levels of some miRs, in particular miR-192 and let-7, between the two cohorts, independent of disease status. These data identify miR-93 is a suitable reference miR for normalizing miRNA levels in TB patients, and highlight how environmental, and possibly ethnic, factors influence miRNA expression levels, demonstrating the necessity of assessing the suitability of reference miRs within the study population. © 2015 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

  15. miRNA Signatures of Insulin Resistance in Obesity.

    Science.gov (United States)

    Jones, Angela; Danielson, Kirsty M; Benton, Miles C; Ziegler, Olivia; Shah, Ravi; Stubbs, Richard S; Das, Saumya; Macartney-Coxson, Donia

    2017-10-01

    Extracellular microRNAs (miRNAs) represent functional biomarkers for obesity and related disorders; this study investigated plasma miRNAs in insulin resistance phenotypes in obesity. One hundred seventy-five miRNAs were analyzed in females with obesity (insulin sensitivity, n = 11; insulin resistance, n = 19; type 2 diabetes, n = 15) and without obesity (n = 12). Correlations between miRNA level and clinical parameters and levels of 15 miRNAs in a murine obesity model were investigated. One hundred six miRNAs were significantly (adjusted P ≤ 0.05) different between controls and at least one obesity phenotype, including miRNAs with the following attributes: previously reported roles in obesity and altered circulating levels (e.g., miR-122, miR-192); known roles in obesity but no reported changes in circulating levels (e.g., miR-378a); and no current reported role in, or association with, obesity (e.g., miR-28-5p, miR-374b, miR-32). The miRNAs in the latter group were found to be associated with extracellular vesicles. Forty-eight miRNAs showed significant correlations with clinical parameters; stepwise regression retained let-7b, miR-144-5p, miR-34a, and miR-532-5p in a model predictive of insulin resistance (R 2  = 0.57, P = 7.5 × 10 -8 ). Both miR-378a and miR-122 were perturbed in metabolically relevant tissues in a murine model of obesity. This study expands on the role of extracellular miRNAs in insulin-resistant phenotypes of obesity and identifies candidate miRNAs not previously associated with obesity. © 2017 The Obesity Society.

  16. Cointegrating MiDaS Regressions and a MiDaS Test

    OpenAIRE

    J. Isaac Miller

    2011-01-01

    This paper introduces cointegrating mixed data sampling (CoMiDaS) regressions, generalizing nonlinear MiDaS regressions in the extant literature. Under a linear mixed-frequency data-generating process, MiDaS regressions provide a parsimoniously parameterized nonlinear alternative when the linear forecasting model is over-parameterized and may be infeasible. In spite of potential correlation of the error term both serially and with the regressors, I find that nonlinear least squares consistent...

  17. miR-192, miR-194 and miR-215: a convergent microRNA network suppressing tumor progression in renal cell carcinoma.

    Science.gov (United States)

    Khella, H W Z; Bakhet, M; Allo, G; Jewett, M A S; Girgis, A H; Latif, A; Girgis, H; Von Both, I; Bjarnason, G A; Yousef, G M

    2013-10-01

    MicroRNAs (miRNAs) play a crucial role in tumor progression and metastasis. We, and others, recently identified a number of miRNAs that are dysregulated in metastatic renal cell carcinoma compared with primary renal cell carcinoma. Here, we investigated three miRNAs that are significantly downregulated in metastatic tumors: miR-192, miR-194 and miR-215. Gain-of-function analyses showed that restoration of their expression decreases cell migration and invasion in renal cell carcinoma cell line models, whereas knockdown of these miRNAs resulted in enhancing cellular migration and invasion abilities. We identified three targets of these miRNAs with potential role in tumor aggressiveness: murine double minute 2, thymidylate synthase, and Smad Interacting protein 1/zinc finger E-box binding homeobox 2. We observed a convergent effect (the same molecule can be targeted by all three miRNAs) and a divergent effect (the same miRNA can control multiple targets) for these miRNAs. We experimentally validated these miRNA-target interactions using three independent approaches. First, we observed that miRNA overexpression significantly reduces the mRNA and protein levels of their targets. In the second, we observed significant reduction of the luciferase signal of a vector containing the 3'UTR of the target upon miRNA overexpression. Finally, we show the presence of inverse correlation between miRNA changes and the expression levels of their targets in patient specimens. We also examined the prognostic significance of miR-215 in renal cell carcinoma. Lower expression of miR-215 is associated with significantly reduced disease-free survival time. These findings were validated on an independent data set from The Cancer Genome Atlas. These results can pave the way to the clinical use of miRNAs as prognostic markers and therapeutic targets.

  18. The regulatory epicenter of miRNAs

    Indian Academy of Sciences (India)

    Bioresource Technology, Council of Scientific & Industrial Research, Palampur 176 061, HP, India. *Corresponding .... miRNA stem and loop regions, interacting with Drosha for .... a double-stranded element, having one strand from the 5′.

  19. Universal MI definition update for cardiovascular disease.

    Science.gov (United States)

    White, Harvey; Thygesen, Kristian; Alpert, Joseph S; Jaffe, Allan

    2014-01-01

    The new third universal definition of myocardial infarction (MI) is based on troponin elevation together with ischemic symptoms, ischemic ECG changes, and imaging evidence. MIs are classified into five types as to whether they are spontaneous, secondary to imbalance between coronary artery blood supply and demand, related to sudden death, or related to revascularization procedures. The definition is based on a rise and/or fall in troponin levels occurring in a clinical setting. There have been modifications over previous definitions with adding intracoronary thrombus as a criterion, adding a new type of MI type 4c, and raising the cutpoint for the diagnosis of MI related to percutaneous coronary intervention to five times the 99(th) percentile upper reference limit and requiring evidence of ischemia or angiographic complications. In clinical practice, trials, and registries, different definitions are used. There is a need for consistency with regard to the definition of MI and the universal definition should be implemented.

  20. miRNAting control of DNA methylation

    Indian Academy of Sciences (India)

    miRNAting control of DNA methylation. ASHWANI ... function and biological process ... Enrichment analysis of the genes methylated by DRM2 for molecular function and biological ... 39(3), June 2014, 365–380, © Indian Academy of Sciences.

  1. miRNAtools: Advanced Training Using the miRNA Web of Knowledge.

    Science.gov (United States)

    Stępień, Ewa Ł; Costa, Marina C; Enguita, Francisco J

    2018-02-16

    Micro-RNAs (miRNAs) are small non-coding RNAs that act as negative regulators of the genomic output. Their intrinsic importance within cell biology and human disease is well known. Their mechanism of action based on the base pairing binding to their cognate targets have helped the development not only of many computer applications for the prediction of miRNA target recognition but also of specific applications for functional assessment and analysis. Learning about miRNA function requires practical training in the use of specific computer and web-based applications that are complementary to wet-lab studies. In order to guide the learning process about miRNAs, we have created miRNAtools (http://mirnatools.eu), a web repository of miRNA tools and tutorials. This article compiles tools with which miRNAs and their regulatory action can be analyzed and that function to collect and organize information dispersed on the web. The miRNAtools website contains a collection of tutorials that can be used by students and tutors engaged in advanced training courses. The tutorials engage in analyses of the functions of selected miRNAs, starting with their nomenclature and genomic localization and finishing with their involvement in specific cellular functions.

  2. Mi-DISCOVERER: A bioinformatics tool for the detection of mi-RNA in human genome.

    Science.gov (United States)

    Arshad, Saadia; Mumtaz, Asia; Ahmad, Freed; Liaquat, Sadia; Nadeem, Shahid; Mehboob, Shahid; Afzal, Muhammad

    2010-11-27

    MicroRNAs (miRNAs) are 22 nucleotides non-coding RNAs that play pivotal regulatory roles in diverse organisms including the humans and are difficult to be identified due to lack of either sequence features or robust algorithms to efficiently identify. Therefore, we made a tool that is Mi-Discoverer for the detection of miRNAs in human genome. The tools used for the development of software are Microsoft Office Access 2003, the JDK version 1.6.0, BioJava version 1.0, and the NetBeans IDE version 6.0. All already made miRNAs softwares were web based; so the advantage of our project was to make a desktop facility to the user for sequence alignment search with already identified miRNAs of human genome present in the database. The user can also insert and update the newly discovered human miRNA in the database. Mi-Discoverer, a bioinformatics tool successfully identifies human miRNAs based on multiple sequence alignment searches. It's a non redundant database containing a large collection of publicly available human miRNAs.

  3. Circulating miR-1, miR-133a, and miR-206 levels are increased after a half-marathon run.

    Science.gov (United States)

    Gomes, Clarissa P C; Oliveira, Getúlio P; Madrid, Bibiano; Almeida, Jeeser A; Franco, Octávio L; Pereira, Rinaldo W

    2014-11-01

    Circulating miRNAs are potential biomarkers that can be important molecules driving cell-to-cell communication. To investigate circulating muscle-specific miRNAs in recreational athletes. Three miRNAs from whole plasma before and after a half-marathon were analyzed by qPCR. MiR-1, -133a, and -206 significantly increased after the race. Increased levels of miRNAs after exercise point to potential biomarkers and to the possibility of being functional players following endurance training. These miRNAs are potential biomarkers of muscle damage or adaptation to exercise.

  4. miR-20b, miR-98, miR-125b-1*, and let-7e* as new potential diagnostic biomarkers in ulcerative colitis

    DEFF Research Database (Denmark)

    Coskun, Mehmet; Bjerrum, Jacob Tveiten; Seidelin, Jakob Benedict

    2013-01-01

    were obtained endoscopically from patients with active UC or CD, quiescent UC or CD, as well as healthy controls. Total RNA was isolated and miRNA expression assessed using the miRNA microarray Geniom Biochip miRNA Homo sapiens (Febit GmbH, Heidelberg, Germany). Data analysis was carried out...... genes involved in various pathways, such as mitogen-activated protein kinase and cytokine signaling, which are both key signaling pathways in UC. CONCLUSION: The present study provides the first evidence that miR-20b, miR-98, miR-125b-1*, and let-7e* are deregulated in patients with UC. The level...

  5. Evaluation of miR-182/miR-100 Ratio for Diagnosis and Survival Prediction in Bladder Cancer.

    Science.gov (United States)

    Chen, Zhanguo; Wu, Lili; Lin, Qi; Shi, Jing; Lin, Xiangyang; Shi, Liang

    2016-09-01

    Abnormal expression of microRNAs (miRNAs) plays an important role in development of several cancer types, including bladder cancer (BCa). However, the relationship between the ratio of miR-181/miR-100 and the prognosis of BCa has not been studied yet. The aim of this study was to evaluate the expression of miR-182, miR-100 and their clinical significance in BCa. Upregulation of miR-182 and down-regulation of miR-100 were validated in tissue specimens of 134 BCa cases compared with 148 normal bladder epithelia (NBE) specimens  using TaqMan-based real-time reverse transcription quantitative PCR (RT-qPCR). The diagnostic and prognostic evaluation of miR-182, miR-100, and miR-182/miR-100 ratio was also performed. miR-182 was upregulated in BCa and miR-100 was down-regulated in BCa compared with NBE (P ratio increased the diagnostic performance, yielding an AUC of 0.981 (97.01% sensitivity and 90.54% specificity). Moreover, miR-182/miR-100 ratio was associated with pT-stage, histological grade, BCa recurrence and carcinoma in situ (P analysis indicated that miR-182/miR-100 ratio was an independent prognostic factor for overall survival (Hazard ratio: 7.142; 95% CI: 2.106 - 9.891; P analysis revealed that high-level of miR-182/miR-100 ratio was significantly correlated with shortened survival time for BCa patients (P ratio may serve as a novel promising biomarker for diagnosis and survival prediction in BCa. Further studies are needed to elucidate the role of miR-182/miR-100 ratio as a non‑invasive diagnostic tool for BCa.

  6. Knock-down of miR-221 and miR-222 in the radiosensitization of breast cancer cells

    International Nuclear Information System (INIS)

    Zhang Chunzhi; Kang Chunsheng; Cao Yongzhen; Pu Peiyu; Lu Zhonghong; Du Yue

    2009-01-01

    Objective: To investigate the radiosensitizing effect of knock-down of miR-221 miR-222 on MCF-7 human breast cancer cells and explore the possible mechanism. Methods: Antisense oligonucleotides of miR-221 and miR-222 (AS-miR-221 and AS-miR-222), mediated by lipofectamine, were transfected to MCF-7 cells to knock down miR-221 and miR-222, Northern blotting was conducted to detect the expression of miR-221 and miR-222 in transfected cells. The cell apoptosis was detected by flow cytometry and Caspase-3 and Caspase-7 activity assay. Clonogenic assay was used to measure the sensitizing enhancement ratio. Target genes of miR-221 and miR-222 relevant to radio-sensitivity were searched using bioinformatics analysis. The targeted protein expression was determined by Western blot analysis. Results: The expression of miR-221 and miR-222 in the AS-miR-221/222 cells determined by Northern blotting was significantly reduced. Compared with the control group, the cell apoptosis and mitotic cell death after the radiation were significantly higher in AS-miR-221/222 cells. The sensitizing enhancement ratio was 1.87. Based on bioinformatics analysis, PTEN was a target gene of miR-221 and miR-222 which could enhance the radiosensitivity of MCF-7 cells. In AS-miR-221/222 cells, the expression of PTEN was up-regulated while pAkt down-regulated. Conclusions: AS-miR-221 and AS-miR-222 may enhance the radiosensitivity of MCF-7 breast cancer cells by up-regulating the expression of PTEN. (authors)

  7. Cortical Morphogenesis during Embryonic Development Is Regulated by miR-34c and miR-204

    DEFF Research Database (Denmark)

    Veno, Morten T.; Veno, Susanne T.; Rehberg, Kati

    2017-01-01

    The porcine brain closely resembles the human brain in aspects such as development and morphology. Temporal miRNA profiling in the developing embryonic porcine cortex revealed a distinct set of miRNAs, including miR-34c and miR-204, which exhibited a highly specific expression profile across...

  8. Decreased miR-128 and increased miR-21 synergistically cause podocyte injury in sepsis.

    Science.gov (United States)

    Wang, Shanshan; Wang, Jun; Zhang, Zengdi; Miao, Hongjun

    2017-08-01

    Glomerular podocytes are injured in sepsis. We studied, in a sepsis patient, whether microRNAs (miRNAs) play a role in the podocyte injury. Podocytes were cultured and treated with lipopolysaccharide (LPS). Filtration barrier function of podocyte was analyzed with albumin influx assay. Nephrin level was analyzed with reverse transcription polymerase chain reaction (RT-PCR) and western blot. MiRNAs were detected using miRNAs PCR Array and in situ hybridization. MiRNA target sites were evaluated with luciferase reporter assays. LPS impaired the filtration barrier function of podocytes. MiR-128 level was decreased and miR-21 level was increased in podocytes in vitro and in the sepsis patient. The decrease in miR-128 was sufficient to induce the loss of nephrin and the impairment of filtration barrier function, while the increase of miR-21 exacerbated the process. Snail and phosphatase and tensin homolog (PTEN) were identified as the targets of miR-128 and miR-21. Decreased miR-128 induced Snail expression, and the increased miR-21 stabilized Snail by regulating the PTEN/Akt/GSK3β pathway. Supplementation of miR-128 and inhibition of miR-21 suppressed Snail expression and prevented the podocyte injury induced by LPS. Our study suggests that decreased miR-128 and increased miR-21 synergistically cause podocyte injury and are the potential therapeutic targets in sepsis.

  9. Shark predation on migrating adult American eels (Anguilla rostrata in the Gulf of St. Lawrence.

    Directory of Open Access Journals (Sweden)

    Mélanie Béguer-Pon

    Full Text Available In an attempt to document the migratory pathways and the environmental conditions encountered by American eels during their oceanic migration to the Sargasso Sea, we tagged eight silver eels with miniature satellite pop-up tags during their migration from the St. Lawrence River in Québec, Canada. Surprisingly, of the seven tags that successfully transmitted archived data, six were ingested by warm-gutted predators, as observed by a sudden increase in water temperature. Gut temperatures were in the range of 20 to 25°C-too cold for marine mammals but within the range of endothermic fish. In order to identify the eel predators, we compared their vertical migratory behavior with those of satellite-tagged porbeagle shark and bluefin tuna, the only endothermic fishes occurring non-marginally in the Gulf of St. Lawrence. We accurately distinguished between tuna and shark by using the behavioral criteria generated by comparing the diving behavior of these two species with those of our unknown predators. Depth profile characteristics of most eel predators more closely resembled those of sharks than those of tuna. During the first days following tagging, all eels remained in surface waters and did not exhibit diel vertical migrations. Three eels were eaten at this time. Two eels exhibited inverse diel vertical migrations (at surface during the day during several days prior to predation. Four eels were eaten during daytime, whereas the two night-predation events occurred at full moon. Although tagging itself may contribute to increasing the eel's susceptibility to predation, we discuss evidence suggesting that predation of silver-stage American eels by porbeagle sharks may represent a significant source of mortality inside the Gulf of St. Lawrence and raises the possibility that eels may represent a reliable, predictable food resource for porbeagle sharks.

  10. Automation of multiple neutral beam injector controls at Lawrence Livermore Laboratory

    International Nuclear Information System (INIS)

    Pollock, G.G.

    1977-01-01

    The computer control system used on the twelve Neutral Beams of the 2XIIB experiment at the Lawrence Livermore Laboratory (LLL) has evolved over the last three years. It is now in its final form and in regular use. It provides automatic data collection, reduction, and graphics presentation, as well as automatic conditioning, automatic normal operation, and processing of calorimeter data. This paper presents an overview of the capabilities and implementation of the current system, a detailed discussion of the automatic conditioning algorithm, and discusses the future directions for neutral beam automation

  11. Environmental Assessment for the vacuum process laboratory (VPL) relocation at the Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    1992-04-01

    This Environmental Assessment (EA) evaluates the potential environmental impacts of relocating a vacuum process laboratory (VPL) from Building 321 to Building 2231 at Lawrence Livermore National Laboratory (LLNL). The VPL provides the latest technology in the field of vacuum deposition of coatings onto various substrates for several weapons-related and energy-related programs at LLNL. Operations within the VPL at LLNL will not be expanded nor reduced by the relocation. No significant environmental impacts are expected as a result of the relocation of the VPL

  12. Evaluation and recommendations on U.C. Lawrence Livermore Labortory Quality Assurance Program

    International Nuclear Information System (INIS)

    Carpenter, F.D.; Horner, M.H.

    1978-01-01

    A study was conducted of the University of California's Lawrence Livermore Laboratory Quality Assurance Program, which focused on training needs and recommendations tailored to the various on-going programs. Specific attention was directed to an assessment of the quality status for the MFTF facility and the capabilities of assigned quality project engineers. Conclusions and recommendations are presented which not only address the purpose of this study, but extend into other areas to provide insight and needs for a total cost effective application of a quality assurance program

  13. Groundwater quality in the Delaware and St. Lawrence River Basins, New York, 2010

    Science.gov (United States)

    Nystrom, Elizabeth A.

    2012-01-01

    Water samples were collected from 10 production and domestic wells in the Delaware River Basin in New York and from 20 production and domestic wells in the St. Lawrence River Basin in New York from August through November 2010 to characterize groundwater quality in the basins. The samples were collected and processed by standard U.S. Geological Survey procedures and were analyzed for 147 physiochemical properties and constituents, including major ions, nutrients, trace elements, pesticides, volatile organic compounds (VOCs), radionuclides, and indicator bacteria.

  14. Mixed waste certification plan for the Lawrence Berkeley Laboratory Hazardous Waste Handling Facility. Revision 1

    International Nuclear Information System (INIS)

    1995-01-01

    The purpose of this plan is to describe the organization and methodology for the certification of mixed waste handled in the Hazardous Waste Handling Facility (HWHF) at Lawrence Berkeley Laboratory (LBL). This plan is composed to meet the requirements found in the Westinghouse Hanford Company (WHC) Solid Waste Acceptance Criteria (WAC) and follows the suggested outline provided by WHC in the letter of April 26, 1990, to Dr. R.H. Thomas, Occupational Health Division, LBL. Mixed waste is to be transferred to the WHC Hanford Site Central Waste Complex and Burial Grounds in Hanford, Washington

  15. Exploratory Research and Development Fund, FY 1990. Report on Lawrence Berkeley Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    1992-05-01

    The Lawrence Berkeley Laboratory Exploratory R&D Fund FY 1990 report is compiled from annual reports submitted by principal investigators following the close of the fiscal year. This report describes the projects supported and summarizes their accomplishments. It constitutes a part of an Exploratory R&D Fund (ERF) planning and documentation process that includes an annual planning cycle, projection selection, implementation, and review. The research areas covered in this report are: Accelerator and fusion research; applied science; cell and molecular biology; chemical biodynamics; chemical sciences; earth sciences; engineering; information and computing sciences; materials sciences; nuclear science; physics and research medicine and radiation biophysics.

  16. Aerial gama ray and magnetic survey: Lawrence Quadrangle of Kansas and Missouri. Final report

    International Nuclear Information System (INIS)

    1980-11-01

    The Lawrence quadrangle covers approximately 7500 square miles in Kansas and Missouri over the western edge of the Ozark Uplift. Sediments in this area are mostly Pennsylvanian and Permian sandstone, shale, limestone, and coal. As mapped, these are the dominant units in the quadrangle. A search of available literature revealed no known uranium deposits. A total of 94 uranium anomalies were detected and are discussed briefly. Most appear to be related to cultural features. Those associated with coal mine tailings appear to be most significant. Magnetic data appears to relate to complexities in the Precambrian basement

  17. Lawrence Berkeley laboratory neutral-beam engineering test facility power-supply system

    International Nuclear Information System (INIS)

    Lutz, I.C.; Arthur, C.A.; deVries, G.J.; Owren, H.M.

    1981-10-01

    The Lawrence Berkeley Laboratory is upgrading the neutral beam source test facility (NBSTF) into a neutral beam engineering test facility (NBETF) with increased capabilities for the development of neutral beam systems. The NBETF will have an accel power supply capable of 170 kV, 70 A, 30 sec pulse length, 10% duty cycle; and the auxiliary power supplies required for the sources. This paper describes the major components, their ratings and capabilities, and the flexibility designed to accomodate the needs of source development

  18. The LBL [Lawrence Berkeley Laboratory] 1-2 GeV synchrotron radiation source

    International Nuclear Information System (INIS)

    Cornacchia, M.

    1987-03-01

    A description is presented of the conceptual design of the 1 to 2 GeV Synchrotron Radiation Source proposed for construction at Lawrence Berkeley Laboratory. This facility is designed to produce ultraviolet and soft x-ray radiation. The accelerator complex consists of an injection system (linac plus booster synchrotron) and a low-emittance storage ring optimized for insertion devices. Eleven straight sections are available for undulators and wigglers, and up to 48 photon beam lines may ultimately emanate from bending magnets. Design features of the radiation source are the high brightness of the photon beams, the very short pulses (tens of picoseconds), and the tunability of the radiation

  19. Public census data on CD-ROM at Lawrence Berkeley Laboratory. Revision 1

    Energy Technology Data Exchange (ETDEWEB)

    Merrill, D.W.

    1992-07-02

    In connection with the Comprehensive Epidemiologic Data Resource (CEDR) and Populations at Risk to Environmental Pollution (PAREP) projects, of the Information and Computing Sciences Division (ICSD) at Lawrence Berkeley Laboratory (LBL), are using public socioeconomic and geographic data files which are available to CEDR and PAREP collaborators via LBL`s computing network. At this time 67 CD-ROM diskettes (approximately 35 gigabytes) are on line via the Unix file server cedrcd.lbl.gov. Most of the files are from the US Bureau of the Census, and most pertain to the 1990 Census of Population and Housing. This paper contains a list of the CD-ROMs available.

  20. Public census data on CD-ROM at Lawrence Berkeley Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Merrill, D.W.

    1992-07-02

    In connection with the Comprehensive Epidemiologic Data Resource (CEDR) and Populations at Risk to Environmental Pollution (PAREP) projects, of the Information and Computing Sciences Division (ICSD) at Lawrence Berkeley Laboratory (LBL), are using public socioeconomic and geographic data files which are available to CEDR and PAREP collaborators via LBL's computing network. At this time 67 CD-ROM diskettes (approximately 35 gigabytes) are on line via the Unix file server cedrcd.lbl.gov. Most of the files are from the US Bureau of the Census, and most pertain to the 1990 Census of Population and Housing. This paper contains a list of the CD-ROMs available.

  1. Law and the Novel: D.H. Lawrence and Robert Musil

    OpenAIRE

    Bell, Michael

    2014-01-01

    How strange that despite the thousands of times I must have read or written the name “Lawrence” I had never till now noticed that it contains, and indeed commences, with the capitalised word Law. The presence of law, at once asserted and unnoticed, has an ironically iconic value within a name which is itself iconic of a view of life, and of literature. For Lawrence, along with Blake and Nietzsche, is above all one of the great antinomians. But the invoking of all these names immediately sugge...

  2. Inertial fusion research at Lawrence Livermore National Laboratory: program status and future applications

    International Nuclear Information System (INIS)

    Meier, W.R.; Hogan, W.J.

    1986-01-01

    The objectives of the Lawrence Livermore National Laboratory (LLNL) Laser Fusion Program are to understand and develop the science and technology required to utilize inertial confinement fusion (ICF) for both military and commercial applications. The results of recent experiments are described. We point out the progress in our laser studies, where we continue to develop and test the concepts, components, and materials for present and future laser systems. While there are many potential commercial applications of ICF, we limit our discussions to electric power production

  3. Inventory and action plan for greenhouse gas emissions and capture in the Lower Saint Lawrence

    International Nuclear Information System (INIS)

    Granger, F.; Avoine, G.; Michon, P.-Y.; Drainville, L.

    2003-01-01

    The authors reported on a project designed to provide farmers with concrete information based on data from their enterprise to develop an action plan for the reduction of greenhouse gas emissions. This project involved completing an inventory of greenhouse gas emissions and capture for seven farms located in the Lower Saint Lawrence region of Quebec. The authors presented a balance sheet and action plan for the region under study. A total of six priorities were identified. They encompassed measures such as the optimization of nitrogen management in agricultural soils, to increasing the capture rate of carbon dioxide, and reducing the use of fossil fuels. 6 refs., 6 figs

  4. Associated Western Universities summer participant program at the Lawrence Livermore National Laboratory, Summer 1997

    Energy Technology Data Exchange (ETDEWEB)

    Williams, B.

    1997-08-01

    The Associated Western Universities, Inc. (AWU) supports a student summer program at Lawrence Livermore National Laboratory (LLNL). This program is structured so that honors undergraduate students may participate in the Laboratory`s research program under direct supervision of senior Laboratory scientists. Included in this report is a list of the AWU participants for the summer of 1997. All students are required to submit original reports of their summer activities in a format of their own choosing. These unaltered student reports constitute the major portion of this report.

  5. Oceanography and Quaternary geology of the St. Lawrence Estuary and the Saguenay Fjord

    International Nuclear Information System (INIS)

    De Vernal, Anne; St-Onge, Guillaume; Gilbert, Denis

    2011-01-01

    The St. Lawrence Estuary is an environment marked by important freshwater discharge and well stratified water masses, recording large seasonal contrast in surface waters from freezing conditions in winter to temperate conditions in summer due to a very strong seasonal cycle in overlying air temperature. High productivity takes place in the pelagic and benthic environments, where a recent trend toward bottom water hypoxia is observed. The area was profoundly marked by the Quaternary glaciations. Thick glaciomarine sequences dating from the last deglaciation are observed in the Estuary and along the shores, whereas a relatively thin layer (a few meters at most) of hemipelagic mud was deposited during the Holocene.

  6. Signal and Image Processing Research at the Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Roberts, R S; Poyneer, L A; Kegelmeyer, L M; Carrano, C J; Chambers, D H; Candy, J V

    2009-06-29

    Lawrence Livermore National Laboratory is a large, multidisciplinary institution that conducts fundamental and applied research in the physical sciences. Research programs at the Laboratory run the gamut from theoretical investigations, to modeling and simulation, to validation through experiment. Over the years, the Laboratory has developed a substantial research component in the areas of signal and image processing to support these activities. This paper surveys some of the current research in signal and image processing at the Laboratory. Of necessity, the paper does not delve deeply into any one research area, but an extensive citation list is provided for further study of the topics presented.

  7. Differential expression of miR-139, miR-486 and miR-21 in breast cancer patients sub-classified according to lymph node status

    DEFF Research Database (Denmark)

    Rask, Lene; Balslev, Eva; Søkilde, Rolf

    2014-01-01

    PURPOSE: Therapeutic decisions in breast cancer are increasingly guided by prognostic and predictive biomarkers. Non-protein-coding microRNAs (miRNAs) have recently been found to be deregulated in breast cancers and, in addition, to be correlated with several clinico-pathological features. One...... of the most consistently up-regulated miRNAs is miR-21. Here, we specifically searched for differentially expressed miRNAs in high-risk breast cancer patients as compared to low-risk breast cancer patients. In the same patients, we also compared miR-21 expression with the expression of its presumed target...... PTEN. METHODS: Both microarray and RT-qPCR techniques were used to assess miRNA expression levels in lymph node-positive and -negative human invasive ductal carcinoma tissues. Simultaneously, PTEN protein expression levels were assessed using immunohistochemistry. RESULTS: miR-486-5p and miR-139-5p...

  8. miR-29b, miR-205 and miR-221 enhance chemosensitivity to gemcitabine in HuH28 human cholangiocarcinoma cells.

    Directory of Open Access Journals (Sweden)

    Kinya Okamoto

    Full Text Available BACKGROUND AND AIMS: Cholangiocarcinoma (CCA is highly resistant to chemotherapy, including gemcitabine (Gem treatment. MicroRNAs (miRNAs are endogenous, non-coding, short RNAs that can regulate multiple genes expression. Some miRNAs play important roles in the chemosensitivity of tumors. Here, we examined the relationship between miRNA expression and the sensitivity of CCA cells to Gem. METHODS: Microarray analysis was used to determine the miRNA expression profiles of two CCA cell lines, HuH28 and HuCCT1. To determine the effect of candidate miRNAs on Gem sensitivity, expression of each candidate miRNA was modified via either transfection of a miRNA mimic or transfection of an anti-oligonucleotide. Ontology-based programs were used to identify potential target genes of candidate miRNAs that were confirmed to affect the Gem sensitivity of CCA cells. RESULTS: HuCCT1 cells were more sensitive to Gem than were HuH28 cells, and 18 miRNAs were differentially expressed whose ratios over ± 2log2 between HuH28 and HuCCT1. Among these 18 miRNAs, ectopic overexpression of each of three downregulated miRNAs in HuH28 (miR-29b, miR-205, miR-221 restored Gem sensitivity to HuH28. Suppression of one upregulated miRNA in HuH28, miR-125a-5p, inhibited HuH28 cell proliferation independently to Gem treatment. Selective siRNA-mediated downregulation of either of two software-predicted targets, PIK3R1 (target of miR-29b and miR-221 or MMP-2 (target of miR-29b, also conferred Gem sensitivity to HuH28. CONCLUSIONS: miRNA expression profiling was used to identify key miRNAs that regulate Gem sensitivity in CCA cells, and software that predicts miRNA targets was used to identify promising target genes for anti-tumor therapies.

  9. Oligoasthenoteratozoospermia and infertility in mice deficient for miR-34b/c and miR-449 loci.

    Directory of Open Access Journals (Sweden)

    Stefano Comazzetto

    2014-10-01

    Full Text Available Male fertility requires the continuous production of high quality motile spermatozoa in abundance. Alterations in all three metrics cause oligoasthenoteratozoospermia, the leading cause of human sub/infertility. Post-mitotic spermatogenesis inclusive of several meiotic stages and spermiogenesis (terminal spermatozoa differentiation are transcriptionally inert, indicating the potential importance for the post-transcriptional microRNA (miRNA gene-silencing pathway therein. We found the expression of miRNA generating enzyme Dicer within spermatogenesis peaks in meiosis with critical functions in spermatogenesis. In an expression screen we identified two miRNA loci of the miR-34 family (miR-34b/c and miR-449 that are specifically and highly expressed in post-mitotic male germ cells. A reduction in several miRNAs inclusive of miR-34b/c in spermatozoa has been causally associated with reduced fertility in humans. We found that deletion of both miR34b/c and miR-449 loci resulted in oligoasthenoteratozoospermia in mice. MiR-34bc/449-deficiency impairs both meiosis and the final stages of spermatozoa maturation. Analysis of miR-34bc-/-;449-/- pachytene spermatocytes revealed a small cohort of genes deregulated that were highly enriched for miR-34 family target genes. Our results identify the miR-34 family as the first functionally important miRNAs for spermatogenesis whose deregulation is causal to oligoasthenoteratozoospermia and infertility.

  10. Early Sámi visual artists - Western fine art meets Sámi culture

    Directory of Open Access Journals (Sweden)

    Tuija Hautala-Hirvioja

    2014-04-01

    Full Text Available Johan Turi (1854–1936, Nils Nilsson Skum (1872–1951 and John Savio (1902–1938 were among the first Sámi visual artists. The production of their art work occurred between the 1910s and the early 1950s. Sámi aesthetics had its basis in folklore, i.e., handicraft or duodji, which did not follow the principle of art for art’s sake but combined beauty and practicality. Art was part of community life. Not until the 1970s was the word daidda, which is Finnish in origin and which means “art”, adopted into the Sámi language. Turi and Skum became famous through their books. They drew and wrote in order to pass the traditional knowledge of their people on to succeeding generations. They also wanted to introduce Sámi life and culture to non-Sámi people. One typical feature of their work is that they depicted Sáminess in a realistic way and sought to strengthen and preserve the Sámi identity through their art. In Turi and Skum’s work, both the documentation of community life and their own personal expression were strongly present and equally important; for this reason their pictures and texts have both practical and aesthetic dimensions. They did not attend school and were self-taught artists. The third pioneer of Sámi visual arts was John Savio, who, unlike the other two, attended secondary school and studied visual arts both independently and under the guidance of a mentor. He expressively combined Western ways of depiction with Sámi subjects. My article examines what made these early Sámi artists change over from Sámi handicraft, duodji, to Western visual arts, how they used Western pictorial conventions in dealing with their Sámi subjects, and the significance of their art for Sámi identity and culture. They lived and worked under cross pressure: the first few decades of the 20th century were characterized by racial theories that denigrated Sámi people, and the period following World War II was marked by demands for

  11. Evaluation of the contamination of marine algae (seaweed) from the St. Lawrence River and likely to be consumed by humans

    Energy Technology Data Exchange (ETDEWEB)

    Phaneuf, D.; Cote, I.; Dumas, P.; Ferron, L.A.; LeBlanc, A. [CHUQ, Sainte-Foy, Quebec (Canada). Centre de Toxicologie du Quebec

    1999-02-01

    The goal of the study was to assess the contamination of marine algae (seaweeds) growing in the St. Lawrence River estuary and Gulf of St. Lawrence and to evaluate the risks to human health from the consumption of these algae. Algae were collected by hand at low tide. A total of 10 sites on the north and south shores of the St. Lawrence as well as in Baie des Chaleurs were sampled. The most frequently collected species of algae were Fucus vesiculosus, Ascophyllum nodosum, Laminaria Longicruris, Palmaria palmata, Ulva lactuca, and Fucus distichus. Alga samples were analyzed for metals iodine, and organochlorines. A risk assessment was performed using risk factors. In general, concentrations in St. Lawrence algae were not very high. Consequently, health risks associated with these compounds in St. Lawrence algae were very low. Iodine concentration, on the other hand, could be of concern with regard to human health. Regular consumption of algae, especially of Laminaria sp., could result in levels of iodine sufficient to cause thyroid problems. For regular consumers, it would be preferable to choose species with low iodine concentrations, such as U. lactuca and P. palmata, in order to prevent potential problems. Furthermore, it would also be important to assess whether preparation for consumption or cooking affects the iodine content of algae. Algae consumption may also have beneficial health effects. Scientific literature has shown that it is a good source of fiber and vitamins, especially vitamin B{sub 12}.

  12. Hindcast storm events in the Bering Sea for the St. Lawrence Island and Unalakleet Regions, Alaska

    Science.gov (United States)

    Erikson, Li H.; McCall, Robert T.; van Rooijen, Arnold; Norris, Benjamin

    2015-01-01

    This study provides viable estimates of historical storm-induced water levels in the coastal communities of Gambell and Savoonga situated on St. Lawrence Island in the Bering Sea, as well as Unalakleet located at the head of Norton Sound on the western coast of Alaska. Gambell, Savoonga, and Unalakleet are small Native Villages that are regularly impacted by coastal storms but where little quantitative information about these storms exists. The closest continuous water-level gauge is at Nome, located more than 200 kilometers from both St. Lawrence Island and Unalakleet. In this study, storms are identified and quantified using historical atmospheric and sea-ice data and then used as boundary conditions for a suite of numerical models. The work includes storm-surge (temporary rise in water levels due to persistent strong winds and low atmospheric pressures) modeling in the Bering Strait region, as well as modeling of wave runup along specified sections of the coast in Gambell and Unalakleet. Modeled historical water levels are used to develop return periods of storm surge and storm surge plus wave runup at key locations in each community. It is anticipated that the results will fill some of the data void regarding coastal flood data in western Alaska and be used for production of coastal vulnerability maps and community planning efforts.

  13. Phallodrilus hallae, a new tubificid oligochaete from the St. Lawrence Great Lakes

    Science.gov (United States)

    Cook, David G.; Hiltunen, Jarl K.

    1975-01-01

    The predominantly marine tubificid genus Phallodrilus is defined, a key to its nine species constructed, and an illustrated description of Phallodrilus hallae n. sp. from the St. Lawrence Great Lakes presented. The species is distinguished from other members of the genus by its well-developed atrial musculature, extensions of which ensheath the posterior prostatic ducts.Phallodrilus hallae n. sp. is a small worm which is widely distributed in the sublittoral and profundal benthos of Lake Superior; lakewide it occurred in mean densities of 50 individuals per square metre. Available records indicate a more restricted distribution in Lake Huron and Georgian Bay. We suggest that P. hallae n. sp. is either a glaciomarine relict species, or that it entered the Great Lakes system at the time of the marine transgression of the St. Lawrence valley. The apparent restriction of P. hallae n. sp. to waters of high quality suggests that it may be a sensitive oligotrophic indicator species.

  14. Catalog of Research Abstracts, 1993: Partnership opportunities at Lawrence Berkeley Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    1993-09-01

    The 1993 edition of Lawrence Berkeley Laboratory`s Catalog of Research Abstracts is a comprehensive listing of ongoing research projects in LBL`s ten research divisions. Lawrence Berkeley Laboratory (LBL) is a major multi-program national laboratory managed by the University of California for the US Department of Energy (DOE). LBL has more than 3000 employees, including over 1000 scientists and engineers. With an annual budget of approximately $250 million, LBL conducts a wide range of research activities, many that address the long-term needs of American industry and have the potential for a positive impact on US competitiveness. LBL actively seeks to share its expertise with the private sector to increase US competitiveness in world markets. LBL has transferable expertise in conservation and renewable energy, environmental remediation, materials sciences, computing sciences, and biotechnology, which includes fundamental genetic research and nuclear medicine. This catalog gives an excellent overview of LBL`s expertise, and is a good resource for those seeking partnerships with national laboratories. Such partnerships allow private enterprise access to the exceptional scientific and engineering capabilities of the federal laboratory systems. Such arrangements also leverage the research and development resources of the private partner. Most importantly, they are a means of accessing the cutting-edge technologies and innovations being discovered every day in our federal laboratories.

  15. Trophic interactions in the St. Lawrence Estuary (Canada): Must the blue whale compete for krill?

    Science.gov (United States)

    Savenkoff, C.; Comtois, S.; Chabot, D.

    2013-09-01

    Inverse methodology was used to construct a mass-balance model of the Lower St. Lawrence Estuary (LSLE) for the 2008-2010 time period. Our first objective was to make an overall description of community structure, trophic interactions, and the effects of fishing and predation on the vertebrate and invertebrate communities of the ecosystem. A second objective was to identify other important predators of krill, and to assess if these compete with blue whales, listed as endangered under the Canadian Species at Risk Act in 2005 (northwest Atlantic population). The Estuary and the Gulf of St. Lawrence are summer feeding grounds for blue whales and other marine mammals. Blue whales eat only euphausiids (krill) and require dense concentrations of prey to meet their energy requirements, which makes them particularly vulnerable to changes in prey availability. In the LSLE, many species from secondary producers (hyperiid amphipods, other macrozooplankton) to top predators (fish, birds, and marine mammals) consumed euphausiids. Consequently, krill predators were found at all consumer trophic levels. However, our results showed that only about 35% of the estimated euphausiid production was consumed by all predator species combined. Euphausiid did not seem to be a restricted resource in the LSLE ecosystem, at least during the study period. The blue whale did not appear to have to compete for krill in the LSLE.

  16. Immunotoxicity in plaice exposed to marine sediments in Baie des Anglais on the St. Lawrence Estuary

    Energy Technology Data Exchange (ETDEWEB)

    Lacroix, A. [Univ. of Quebec, Montreal, Quebec (Canada); Nagler, J.; Lee, K.; Lebeuf, M.; Cyr, D. [Fisheries and Oceans Canada, Mont-Joli, Quebec (Canada); Fournier, M. [Univ. of Quebec, Montreal, Quebec (Canada)]|[Fisheries and Oceans Canada, Mont-Joli, Quebec (Canada)

    1995-12-31

    The sediments of Baie des Anglais on the St. Lawrence Estuary have a history of environmental contamination. The purpose of the present study was to determine whether or not the immune system of American Plaice (Hippoglossoides Platessoides) could be affected following in-situ exposure at three different sites in and near Baie des Anglais. These sites vary with their proximity to local industry, Sites 1 and 2 (within the bay) being the closest and Site 3 (outside the bay) the furthest away. Fishes placed in cages at each site for three weeks, displayed head kidney cell immune responses (i.e., phagocytosis) modifications indicating that Site 1 was most immunotoxic and site 3 the least. Sediment chemical analysis show a gradient in contaminant concentrations with the highest levels recorded at Site 1, about 10-fold less at Site 2 and 100-fold less at Site 3. Organics predominated (PAHs, PCBs, PCDFs) with heavy metal concentrations low and representative of background levels for the St. Lawrence Estuary. The results obtained indicate that contaminants present in the sediments are bioavailable to fish and significantly affect their immune system.

  17. Lawrence Livermore National Laboratory Experimental Test Site (Site 300) Potable Water System Operations Plan

    Energy Technology Data Exchange (ETDEWEB)

    Ocampo, Ruben P. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Bellah, Wendy [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2016-03-04

    The existing Lawrence Livermore National Laboratory (LLNL) Site 300 drinking water system operation schematic is shown in Figures 1 and 2 below. The sources of water are from two Site 300 wells (Well #18 and Well #20) and San Francisco Public Utilities Commission (SFPUC) Hetch-Hetchy water through the Thomas shaft pumping station. Currently, Well #20 with 300 gallons per minute (gpm) pump capacity is the primary source of well water used during the months of September through July, while Well #18 with 225 gpm pump capacity is the source of well water for the month of August. The well water is chlorinated using sodium hypochlorite to provide required residual chlorine throughout Site 300. Well water chlorination is covered in the Lawrence Livermore National Laboratory Experimental Test Site (Site 300) Chlorination Plan (“the Chlorination Plan”; LLNL-TR-642903; current version dated August 2013). The third source of water is the SFPUC Hetch-Hetchy Water System through the Thomas shaft facility with a 150 gpm pump capacity. At the Thomas shaft station the pumped water is treated through SFPUC-owned and operated ultraviolet (UV) reactor disinfection units on its way to Site 300. The Thomas Shaft Hetch- Hetchy water line is connected to the Site 300 water system through the line common to Well pumps #18 and #20 at valve box #1.

  18. miR482 and Its Isoforms in Plants

    Directory of Open Access Journals (Sweden)

    Abdil Hakan EREN

    2016-09-01

    Full Text Available In plants, miR482 family members are generally 22-nucleotide long, distinguishing from other microRNA (miRNA families by their extraordinary and diverse sequence structures. Studies showed that miRNA482 is related to NBLRR (Nucleotide binding-site leucine-rich repeat genes conferring resistance to disease in plants. There are different coded NB-LRR genes which are considered as the part immune response assisting the recognition of pathogens in plant genomes. NB-LRR proteins are mostly related to effector – triggering immune system against pathogens. The main immune receptors in plants are PRR (Pattern recoginition receptor and R (Resistance proteins. R proteins code for immune system proteins by NB-LRR activity. miR482, miR1448, slmiR2118 and ath-miR472 are disease resistance related miRNAs. In several studies, miR482 was found to be a homolog of miR1448 and phylogenetic analyses showed that miR1448 is formed by tandem duplication of miR482. While suppression of miR482 results in plant susceptibility to pathogens, miR482 was considered to play role in nodulation and mycorrhizal processes of soya roots. Increasing evidences exhibit that miR482 is critical in disease resistance against pathogen attacks.

  19. MDRL lncRNA regulates the processing of miR-484 primary transcript by targeting miR-361.

    Directory of Open Access Journals (Sweden)

    Kun Wang

    2014-07-01

    Full Text Available Long noncoding RNAs (lncRNAs are emerging as new players in gene regulation, but whether lncRNAs operate in the processing of miRNA primary transcript is unclear. Also, whether lncRNAs are involved in the regulation of the mitochondrial network remains to be elucidated. Here, we report that a long noncoding RNA, named mitochondrial dynamic related lncRNA (MDRL, affects the processing of miR-484 primary transcript in nucleus and regulates the mitochondrial network by targeting miR-361 and miR-484. The results showed that miR-361 that predominantly located in nucleus can directly bind to primary transcript of miR-484 (pri-miR-484 and prevent its processing by Drosha into pre-miR-484. miR-361 is able to regulate mitochondrial fission and apoptosis by regulating miR-484 levels. In exploring the underlying molecular mechanism by which miR-361 is regulated, we identified MDRL and demonstrated that it could directly bind to miR-361 and downregulate its expression levels, which promotes the processing of pri-miR-484. MDRL inhibits mitochondrial fission and apoptosis by downregulating miR-361, which in turn relieves inhibition of miR-484 processing by miR-361. Our present study reveals a novel regulating model of mitochondrial fission program which is composed of MDRL, miR-361 and miR-484. Our work not only expands the function of the lncRNA pathway in gene regulation but also establishes a new mechanism for controlling miRNA expression.

  20. Cell type-specific deficiency of c-kit gene expression in mutant mice of mi/mi genotype.

    Science.gov (United States)

    Isozaki, K.; Tsujimura, T.; Nomura, S.; Morii, E.; Koshimizu, U.; Nishimune, Y.; Kitamura, Y.

    1994-01-01

    The mi locus of mice encodes a novel member of the basic-helix-loop-helix-leucine zipper protein family of transcription factors (hereafter called mi factor). In addition to microphthalmus, osteopetrosis, and lack of melanocytes, mice of mi/mi genotype are deficient in mast cells. Since the c-kit receptor tyrosine kinase plays an important role in the development of mast cells, and since the c-kit expression by cultured mast cells from mi/mi mice is deficient in both mRNA and protein levels, the mast cell deficiency of mi/mi mice has been attributed at least in part to the deficient expression of c-kit. However, it remained to be examined whether the c-kit expression was also deficient in tissues of mi/mi mice. In the present study, we examined the c-kit expression by mi/mi skin mast cells using in situ hybridization and immunohistochemistry. Moreover, we examined the c-kit expression by various cells other than mast cells in tissues of mi/mi mice. We found that the c-kit expression was deficient in mast cells but not in erythroid precursors, testicular germ cells, and neurons of mi/mi mice. This suggested that the regulation of the c-kit transcription by the mi factor was dependent on cell types. Mice of mi/mi genotype appeared to be a useful model to analyze the function of transcription factors in the whole-animal level. Images Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 PMID:7524330

  1. FLOODPLAIN, Monroe County, Michigan, USA

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — The Floodplain Mapping/Redelineation study deliverables depict and quantify the flood risks for the study area. The primary risk classifications used are the...

  2. TERRAIN, MONROE COUNTY, Michigan USA

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Terrain data, as defined in FEMA Guidelines and Specifications, Appendix M: Data Capture Standards, describes the digital topographic data that was used to create...

  3. BASEMAP, MONROE COUNTY, KENTUCKY USA

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — FEMA Framework Basemap datasets comprise six of the seven FGDC themes of geospatial data that are used by most GIS applications (Note: the seventh framework theme,...

  4. Characterization of the Merkel Cell Carcinoma miRNome

    Directory of Open Access Journals (Sweden)

    Matthew S. Ning

    2014-01-01

    Full Text Available MicroRNAs have been implicated in various skin cancers, including melanoma, squamous cell carcinoma, and basal cell carcinoma; however, the expression of microRNAs and their role in Merkel cell carcinoma (MCC have yet to be explored in depth. To identify microRNAs specific to MCC (MCC-miRs, next-generation sequencing (NGS of small RNA libraries was performed on different tissue samples including MCCs, other cutaneous tumors, and normal skin. Comparison of the profiles identified several microRNAs upregulated and downregulated in MCC. For validation, their expression was measured via qRT-PCR in a larger group of MCC and in a comparison group of non-MCC cutaneous tumors and normal skin. Eight microRNAs were upregulated in MCC: miR-502-3p, miR-9, miR-7, miR-340, miR-182, miR-190b, miR-873, and miR-183. Three microRNAs were downregulated: miR-3170, miR-125b, and miR-374c. Many of these MCC-miRs, the miR-183/182/96a cistron in particular, have connections to tumorigenic pathways implicated in MCC pathogenesis. In situ hybridization confirmed that the highly expressed MCC-miR, miR-182, is localized within tumor cells. Furthermore, NGS and qRT-PCR reveal that several of these MCC-miRs are highly expressed in the patient-derived MCC cell line, MS-1. These data indicate that we have identified a set of MCC-miRs with important implications for MCC research.

  5. miReg: a resource for microRNA regulation

    Directory of Open Access Journals (Sweden)

    Barh Debmalya

    2010-03-01

    Full Text Available MicroRNAs (miRNAs/miRs are important cellular components that regulate gene expression at posttranscriptional level. Various upstream components regulate miR expression and any deregulation causes disease conditions. Therefore, understanding of miR regulatory network both at upstream and downstream level is crucial and a resource on this aspect will be helpful. Currently available miR databases are mostly related to downstream targets, sequences, or diseases. But as of now, no database is available that provides a complete picture of miR regulation in a specific condition.

  6. Evaluation of miR-21 and miR-375 as prognostic biomarkers in esophageal cancer

    DEFF Research Database (Denmark)

    Winther, Mette; Alsner, Jan; Tramm, Trine

    2015-01-01

    analyses identified miR-21 as an independent prognostic marker for DSS in EAC [HR 3.52 (95% CI 1.06-11.69)]. High miR-375 was not correlated with improved prognosis in either histology. However, Forest plots demonstrated that both miR-21 and miR-375 were of prognostic impact in ESCC. CONCLUSION...... chemotherapy were analyzed. Expression levels of miR-21 and miR-375 were quantified using Affymetrix GeneChip miRNA 1.0 Array. The Cox proportional hazards model was used to assess the correlation of miR-21 and miR-375 with disease-specific survival (DSS) and overall survival (OS). Forest plots were performed...... to evaluate the prognostic impact of miR-21 and miR-375 in the present study and previously published reports. RESULTS: In ESCC, patients with miR-21 expression levels above median showed a trend towards poorer DSS and OS. When dividing miR-21 expression by tertiles, high levels of miR-21 significantly...

  7. miR-371, miR-138, miR-544, miR-145, and miR-214 could modulate Th1/Th2 balance in asthma through the combinatorial regulation of Runx3.

    Science.gov (United States)

    Qiu, Yu-Ying; Zhang, Ying-Wei; Qian, Xiu-Fen; Bian, Tao

    2017-01-01

    Asthma is tightly related to the imbalance of Th1/Th2 cells, and Runx3 plays a pivotal role in the differentiation of T helper cells. The present study aimed to investigate dysregulated microRNAs that may target Runx3 in CD4 + T cells from asthmatic patients and reveal Runx3 function in Th1/Th2 balance regulation. We detected the levels of Th1- and Th2-related cytokines by ELISA and analyzed the differentiation marker gene of T helper cells by qRT-PCR. Results indicated that an imbalance of Th1/Th2 cells was present in our asthmatic subject. Runx3 expression was reduced in the CD4 + T cells from asthmatic patients. Overexpression of Runx3 could restore the Th1/Th2 balance. After performing microRNA microarray assay, we found a series of microRNAs that were considerably altered in the CD4 + T cells from asthmatic patients. Among these upregulated microRNAs, eight microRNAs that may target Runx3 were selected by bioinformatics prediction. Five microRNAs, namely miR-371, miR-138, miR-544, miR-145, and miR-214, were confirmed by qRT-PCR and selected as candidate microRNAs. Luciferase reporter assay showed that these five microRNAs could directly target the 3'-UTR of Runx3. However, only simultaneous inhibition of these five microRNAs could alter the expression of Runx3. Most importantly, only simultaneous inhibition could improve the Th1/Th2 balance. Thus, we suggest that miR-371, miR-138, miR-544, miR-145, and miR-214 can modulate the Th1/Th2 balance in asthma by regulating Runx3 in a combinatorial manner.

  8. A path-based measurement for human miRNA functional similarities using miRNA-disease associations

    Science.gov (United States)

    Ding, Pingjian; Luo, Jiawei; Xiao, Qiu; Chen, Xiangtao

    2016-09-01

    Compared with the sequence and expression similarity, miRNA functional similarity is so important for biology researches and many applications such as miRNA clustering, miRNA function prediction, miRNA synergism identification and disease miRNA prioritization. However, the existing methods always utilized the predicted miRNA target which has high false positive and false negative to calculate the miRNA functional similarity. Meanwhile, it is difficult to achieve high reliability of miRNA functional similarity with miRNA-disease associations. Therefore, it is increasingly needed to improve the measurement of miRNA functional similarity. In this study, we develop a novel path-based calculation method of miRNA functional similarity based on miRNA-disease associations, called MFSP. Compared with other methods, our method obtains higher average functional similarity of intra-family and intra-cluster selected groups. Meanwhile, the lower average functional similarity of inter-family and inter-cluster miRNA pair is obtained. In addition, the smaller p-value is achieved, while applying Wilcoxon rank-sum test and Kruskal-Wallis test to different miRNA groups. The relationship between miRNA functional similarity and other information sources is exhibited. Furthermore, the constructed miRNA functional network based on MFSP is a scale-free and small-world network. Moreover, the higher AUC for miRNA-disease prediction indicates the ability of MFSP uncovering miRNA functional similarity.

  9. "Seed-Milarity" confers to hsa-miR-210 and hsa-miR-147b similar functional activity.

    Directory of Open Access Journals (Sweden)

    Thomas Bertero

    Full Text Available Specificity of interaction between a microRNA (miRNA and its targets crucially depends on the seed region located in its 5'-end. It is often implicitly considered that two miRNAs sharing the same biological activity should display similarity beyond the strict six nucleotide region that forms the seed, in order to form specific complexes with the same mRNA targets. We have found that expression of hsa-miR-147b and hsa-miR-210, though triggered by different stimuli (i.e. lipopolysaccharides and hypoxia, respectively, induce very similar cellular effects in term of proliferation, migration and apoptosis. Hsa-miR-147b only shares a "minimal" 6-nucleotides seed sequence with hsa-miR-210, but is identical with hsa-miR-147a over 20 nucleotides, except for one base located in the seed region. Phenotypic changes induced after heterologous expression of miR-147a strikingly differ from those induced by miR-147b or miR-210. In particular, miR-147a behaves as a potent inhibitor of cell proliferation and migration. These data fit well with the gene expression profiles observed for miR-147b and miR-210, which are very similar, and the gene expression profile of miR-147a, which is distinct from the two others. Bioinformatics analysis of all human miRNA sequences indicates multiple cases of miRNAs from distinct families exhibiting the same kind of similarity that would need to be further characterized in terms of putative functional redundancy. Besides, it implies that functional impact of some miRNAs can be masked by robust expression of miRNAs belonging to distinct families.

  10. EL CIRCO Y MI DILEMA

    Directory of Open Access Journals (Sweden)

    Martalucía Tamayo

    2015-03-01

    habilidades excepcionales, temo que lo que se esté buscando (¿o logrando? es un aplauso lleno de compasión o lástima por su condición de “seres diferentes”; o más bien agradados por haber presenciado “un fenómeno” digno de ser exhibido para la curiosidad morbosa de la gente. Ojalá no haya sido por eso. Tengo el mismo sentimiento de rechazo a los movimientos tipo “Teletón”, que suelen recoger ayuda económica mediante la explotación de un sentimiento de “compasión y lástima”. He luchado toda mi vida para que la sociedad no olvide el valor de la diferencia, el respeto a ella, el derecho a ser y vivir diferente; y hemos trabajado por muchos años para que esas personas “diferentes” sean (seamos “sujetos de derechos” igual que cualquier otro ser humano. Lo triste, y he ahí mi dilema, es que muchas personas del público comentan “pobrecitos, tan tiernos, al menos estos dos tienen empleo y viven de algo” y me digo que es verdad; son de los pocos que quizás logren sobrevivir económicamente bien en un mundo que está lejos de ser fácil para ellos. Pero ahí surge el dilema y el temor de que la sociedad aprenda a ver las cosas de manera equivocada. Y... ¿la dignidad? ¿Dónde queda la concientización o sensibilización de la sociedad? ¿Dónde queda el respeto a la diferencia? ¿A quién podría gustarle ser exhibido como fenómeno? ¿Alguien se ha preguntado el drama que vive el “ser diferente” en un mundo que no está diseñado para todo el mundo?...

  11. miRNAs in Alzheimer Disease - A Therapeutic Perspective.

    Science.gov (United States)

    Gupta, Priya; Bhattacharjee, Surajit; Sharma, Ashish Ranjan; Sharma, Garima; Lee, Sang-Soo; Chakraborty, Chiranjib

    2017-01-01

    Alzheimer's disease is a neurodegenerative disorder which generally affects people who are more than 60 years of age. The disease is clinically characterised by dementia, loss of cognitive functions and massive neurodegeneration. The presence of neurofibrilary tangles and amyloid plaques in the hippocampal region of the brain are the hallmarks of the disease. Current therapeutic approaches for the treatment of Alzheimer's disease are symptomatic and disease modifying, none of which provide any permanent solution or cure for the disease. Dysregulation of miRNAs is one of the major causes of neurodegeneration. In the present review, the roles of different miRNAs such as miR-9, miR-107, miR-29, miR-34, miR-181, miR-106, miR-146a, miR132, miR124a, miR153 has been discussed in detail in the pathogenesis of various neurodegenerative diseases with special focus on AD. The probability of miRNAs as an alternative and more sensitive approach for detection and management of the AD has also been discussed. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

  12. Current perspectives in microRNAs (miRNA)

    CERN Document Server

    Ying, Shao-Yao

    2008-01-01

    In this book, many new perspectives of the miRNA research are reviewed and discussed. These new findings provide significant insight into the various mechanisms of miRNAs and offer a great opportunity in developing new therapeutic interventions.

  13. Circular RNA and miR-7 in Cancer

    DEFF Research Database (Denmark)

    Hansen, Thomas Birkballe; Kjems, Jørgen; Damgaard, Christian Kroun

    2013-01-01

    MicroRNAs (miRNA) play important roles in fine-tuning gene expression and are often deregulated in cancer. The identification of competing endogenous RNA and circular RNA (circRNA) as important regulators of miRNA activity underscores the increasing complexity of ncRNA-mediated regulatory networks....... Particularly, the recently identified circular RNA, ciRS-7, which acts as a designated miR-7 inhibitor/sponge, has conceptually changed the mechanistic understanding of miRNA networks. As miR-7 modulates the expression of several oncogenes, disclosing the regulation of miR-7 activity will likely advance...... the understanding of various cancer etiologies. Here, we review the current knowledge about the ciRS-7/miR-7 axis in cancer-related pathways and discuss possible models explaining the relevance of coexpressing miR-7 along with a circRNA inhibitor....

  14. miR-24 and miR-205 expression is dependent on HPV onco-protein expression in keratinocytes

    Energy Technology Data Exchange (ETDEWEB)

    McKenna, Declan J., E-mail: dj.mckenna@ulster.ac.uk [Biomedical Sciences Research Institute, University of Ulster, Coleraine, Co. Derry BT52 1SA (United Kingdom); Centre for Cancer Research and Cell Biology, School of Medicine, Dentistry and Biomedical Science, Queen' s University Belfast, Belfast BT9 7BL (United Kingdom); Patel, Daksha, E-mail: d.patel@qub.ac.uk [Centre for Cancer Research and Cell Biology, School of Medicine, Dentistry and Biomedical Science, Queen' s University Belfast, Belfast BT9 7BL (United Kingdom); McCance, Dennis J., E-mail: d.mccance@qub.ac.uk [Centre for Cancer Research and Cell Biology, School of Medicine, Dentistry and Biomedical Science, Queen' s University Belfast, Belfast BT9 7BL (United Kingdom)

    2014-01-05

    A screen of microRNA (miRNA) expression following differentiation in human foreskin keratinocytes (HFKs) identified changes in several miRNAs, including miR-24 and miR-205. We investigated how expression of Human Papilloma Virus Type-16 (HPV16) onco-proteins E6 and E7 affected expression of miR-24 and miR-205 during proliferation and differentiation of HFKs. We show that the induction of both miR-24 and miR-205 observed during differentiation of HFKs is lost in HFKs expressing E6 and E7. We demonstrate that the effect on miR-205 is due to E7 activity, as miR-205 expression is dependent on pRb expression. Finally, we provide evidence that miR-24 effects in the cell may be due to targeting of cyclin dependent kinase inhibitor p27. In summary, these results indicate that expression of both miR-24 and miR-205 are impacted by E6 and/or E7 expression, which may be one mechanism by which HPV onco-proteins can disrupt the balance between proliferation and differentiation in keratinocytes. - Highlights: • miR-24 and miR-205 are induced during keratinocyte differentiation. • This induction is lost in keratinocytes expressing HPV onco-proteins E6 and E7. • miR-205 is dependent upon pRb expression. • miR-24 targets p27 in cycling keratinocytes.

  15. miR-24 and miR-205 expression is dependent on HPV onco-protein expression in keratinocytes

    International Nuclear Information System (INIS)

    McKenna, Declan J.; Patel, Daksha; McCance, Dennis J.

    2014-01-01

    A screen of microRNA (miRNA) expression following differentiation in human foreskin keratinocytes (HFKs) identified changes in several miRNAs, including miR-24 and miR-205. We investigated how expression of Human Papilloma Virus Type-16 (HPV16) onco-proteins E6 and E7 affected expression of miR-24 and miR-205 during proliferation and differentiation of HFKs. We show that the induction of both miR-24 and miR-205 observed during differentiation of HFKs is lost in HFKs expressing E6 and E7. We demonstrate that the effect on miR-205 is due to E7 activity, as miR-205 expression is dependent on pRb expression. Finally, we provide evidence that miR-24 effects in the cell may be due to targeting of cyclin dependent kinase inhibitor p27. In summary, these results indicate that expression of both miR-24 and miR-205 are impacted by E6 and/or E7 expression, which may be one mechanism by which HPV onco-proteins can disrupt the balance between proliferation and differentiation in keratinocytes. - Highlights: • miR-24 and miR-205 are induced during keratinocyte differentiation. • This induction is lost in keratinocytes expressing HPV onco-proteins E6 and E7. • miR-205 is dependent upon pRb expression. • miR-24 targets p27 in cycling keratinocytes

  16. Lawrence Berkeley National Laboratory 1995 site environmental report: Volume 2, Data appendix

    International Nuclear Information System (INIS)

    1996-07-01

    Ernest Orlando Lawrence Berkeley National Laboratory presents Volume II, Data Appendix as a reference document to supplement the 1995 Site Environmental Report. Volume II contains the raw environmental monitoring and sampling data used to generate many of the summary results included in the main report. Supplemental data is provided for sitewide activities involving the media of stack and ambient air quality, rainwater, surface water, stormwater, wastewater, and soil and sediment. Volume II also contains supplemental data on the special preoperational monitoring study for the new Hazardous Waste Handling Facility. The Table of Contents provides a cross-reference to the data tables of the main report and this appendix. Data are given in System International (SI) units

  17. Barium fluoride surface preparation, analysis and UV reflective coatings at Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    Wuest, C.R.

    1992-01-01

    Lawrence Livermore National Laboratory (LLNL) has begun a program of study on barium fluoride scintillating crystals for the Barium Fluoride Electromagnetic Calorimeter Collaboration. This program has resulted in a number of significant improvements in the mechanical processing, polishing and coating of barium fluoride crystals. Techniques have been developed using diamond-loaded pitch lapping that can produce 15 angstrom RMS surface finishes over large areas. These lapped surfaces have been shown to be crystalline using Rutherford Back-scattering (RBS). Also, special polishing fixtures have been designed based on mounting technology developed for the 1.1 m diameter optics used in LLNL's Nova Laser. These fixtures allow as many as five 25--50 cm long barium fluoride crystals to be polished and lapped at a time with the necessary tolerances for the 16,000 crystal Barium Fluoride Calorimeter. In addition, results will be presented on coating barium fluoride with UV reflective layers of magnesium fluoride and aluminum

  18. Construction and operation of replacement hazardous waste handling facility at Lawrence Berkeley Laboratory

    International Nuclear Information System (INIS)

    1992-09-01

    The US Department of Energy (DOE) has prepared an environmental assessment (EA), DOE/EA-0423, for the construction and operation of a replacement hazardous waste handling facility (HWHF) and decontamination of the existing HWHF at Lawrence Berkeley Laboratory (LBL), Berkeley, California. The proposed facility would replace several older buildings and cargo containers currently being used for waste handling activities and consolidate the LBL's existing waste handling activities in one location. The nature of the waste handling activities and the waste volume and characteristics would not change as a result of construction of the new facility. Based on the analysis in the EA, DOE has determined that the proposed action would not constitute a major Federal action significantly affecting the quality of the human environment within the meaning of the National Environmental Policy Act (NEPA) of 1969, 42 USC. 4321 et seq. Therefore, an environmental impact statement is not required

  19. Lawrence Livermore National Laboratory low-level waste systems performance assessment

    International Nuclear Information System (INIS)

    1990-11-01

    This Low-Level Radioactive Waste (LLW) Systems Performance Assessment (PA) presents a systematic analysis of the potential risks posed by the Lawrence Livermore National Laboratory (LLNL) waste management system. Potential risks to the public and environment are compared to established performance objectives as required by DOE Order 5820.2A. The report determines the associated maximum individual committed effective dose equivalent (CEDE) to a member of the public from LLW and mixed waste. A maximum annual CEDE of 0.01 mrem could result from routine radioactive liquid effluents. A maximum annual CEDE of 0.003 mrem could result from routine radioactive gaseous effluents. No other pathways for radiation exposure of the public indicated detectable levels of exposure. The dose rate, monitoring, and waste acceptance performance objectives were found to be adequately addressed by the LLNL Program. 88 refs., 3 figs., 17 tabs

  20. Solid modeling research at Lawrence Livermore National Laboratory: 1982-1985

    International Nuclear Information System (INIS)

    Kalibjian, J.R.

    1985-01-01

    The Lawrence Livermore National Laboratory has sponsored solid modeling research for the past four years to assess this new technology and to determine its potential benefits to the Nuclear Weapons Complex. We summarize here the results of five projects implemented during our effort. First, we have installed two solid modeler codes, TIPS-1 (Technical Information Processing System-1) and PADL-2 (Part and Assembly Description Language), on the Laboratory's CRAY-1 computers. Further, we have extended the geometric coverage and have enhanced the graphics capabilities of the TIPS-1 modeler. To enhance solid modeler performance on our OCTOPUS computer system, we have also developed a method to permit future use of the Laboratory's network video system to provide high-resolution, shaded images at users' locations. Finally, we have begun to implement code that will link solid-modeler data bases to finite-element meshing codes

  1. Environmental monitoring at the Lawrence Livermore National Laboratory: 1986 annual report

    International Nuclear Information System (INIS)

    Holland, R.C.; Buddemeier, R.W.; Brekke, D.D.

    1987-04-01

    This report documents the results of the environmental monitoring program at the Lawrence Livermore National Laboratory (LLNL) for 1986. To evaluate the effect of LLNL operations on the local environment, measurements of direct radiation and a variety of radionuclides and chemical pollutants in ambient air, soil, surface water, groundwater, vegetation, milk, foodstuff, and sewage effluents were made at both the Livermore site and nearby Site 300. This report was prepared to meet the requirements of DOE Order 5484.1. Evaluations are made of LLNL's compliance with all applicable guides, standards, and limits for radiological and nonradiological releases to the environment. The data indicate that no releases in excess of the applicable standards were made during 1986, and that LLNL operations had no adverse environmental impact

  2. Shark predation on migrating adult american eels (Anguilla rostrata) in the Gulf of St. Lawrence

    DEFF Research Database (Denmark)

    Béguer-Pon, Mélanie; Benchetrit, José; Castonguay, Martin

    2012-01-01

    to identify the eel predators, we compared their vertical migratory behavior with those of satellite-tagged porbeagle shark and bluefin tuna, the only endothermic fishes occurring non-marginally in the Gulf of St. Lawrence. We accurately distinguished between tuna and shark by using the behavioral criteria....... Surprisingly, of the seven tags that successfully transmitted archived data, six were ingested by warm-gutted predators, as observed by a sudden increase in water temperature. Gut temperatures were in the range of 20 to 25°C--too cold for marine mammals but within the range of endothermic fish. In order...... generated by comparing the diving behavior of these two species with those of our unknown predators. Depth profile characteristics of most eel predators more closely resembled those of sharks than those of tuna. During the first days following tagging, all eels remained in surface waters and did not exhibit...

  3. Title I conceptual design for Pit 6 landfill closure at Lawrence Livermore National Laboratory Site 300

    International Nuclear Information System (INIS)

    MacDonnell, B.A.; Obenauf, K.S.

    1996-08-01

    The objective of this design project is to evaluate and prepare design and construction documents for a closure cover cap for the Pit 6 Landfill located at Lawrence Livermore National Laboratory Site 300. This submittal constitutes the Title I Design (Conceptual Design) for the closure cover of the Pit 6 Landfill. A Title I Design is generally 30 percent of the design effort. Title H Design takes the design to 100 percent complete. Comments and edits to this Title I Design will be addressed in the Title II design submittal. Contents of this report are as follows: project background; design issues and engineering approach; design drawings; calculation packages; construction specifications outline; and construction quality assurance plan outline

  4. Distribution of some radionuclides in the St. Lawrence estuary, Quebec, Canada

    International Nuclear Information System (INIS)

    Serodes, J.B.; Roy, J.C.

    1983-01-01

    The distribution of γ-emitting radionuclides in the St. Lawrence estuary was studied in 1978 and 1979, by means of double sampling and the flocculation and centrifugation of very large volumes of water. Eleven radionuclides were detected, originating from a variety of sources, including soil erosion and nuclear weapons testing. The concentrations measured in 1979 were higher than those of 1978; the 21st Chinese nuclear test could be responsible for the increase of some radionuclides. Concentrations decrease markedly from the freshwater part to the marine region of the estuary. Dilution by oceanic waters, relative affinity with suspended matter and radioactive decay are the principal mechanisms involved in the distribution patterns. Cesium-137, 144 Ce, 226 Ra, 228 Ra and 228 Th are strongly associated with suspended matter, while about two thirds of 7 Be, 106 Ru and 235 U are present in the liquid phase. Results suggest that 235 U is released from sediments in the maximum turbidity zone

  5. Waste minimization activities in the Materials Fabrication Division at Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    Dini, J.W.

    1991-08-01

    The mission of the Materials Fabrication Division (MFD) is to provide fabrication services and technology in support of all programs at Lawrence Livermore National Laboratory (LLNL). MFD involvement is called for when fabrication activity requires levels of expertise, technology, equipment, process development, hazardous processes, security, or scheduling that is typically not commercially available. Customers are encouraged to utilize private industry for fabrication activity requiring routine processing or for production applications. Our waste minimization (WM) program has been directed at source reduction and recycling in concert with the working definition of waste minimization used by EPA. The principal focus of WM activities has been on hazardous wastes as defined by RCRA, however, all pollutant emissions into air, water and land are being considered as part of the program. The incentives include: (1) economics, (2) regulatory conformance, (3) public image and (4) environmental concern. This report discusses the waste minimization program at LLNL

  6. Modernist physics waves, particles, and relativities in the writings of Virginia Woolf and D. H. Lawrence

    CERN Document Server

    Crossland, Rachel

    2018-01-01

    Modernist Physics takes as its focus the ideas associated with three scientific papers published by Albert Einstein in 1905, considering the dissemination of those ideas both within and beyond the scientific field, and exploring the manifestation of similar ideas in the literary works of Virginia Woolf and D. H. Lawrence. Drawing on Gillian Beer's suggestion that literature and science 'share the moment's discourse', Modernist Physics seeks both to combine and to distinguish between the two standard approaches within the field of literature and science: direct influence and the zeitgeist. The book is divided into three parts, each of which focuses on the ideas associated with one of Einstein's papers. Part I considers Woolf in relation to Einstein's paper on light quanta, arguing that questions of duality and complementarity had a wider cultural significance in the early twentieth century than has yet been acknowledged, and suggesting that Woolf can usefully be considered a complementary, rather than a duali...

  7. Environmental monitoring at the Lawrence Livermore National Laboratory: Annual report, 1987

    International Nuclear Information System (INIS)

    Holland, R.C.; Brekke, D.D.

    1988-04-01

    This report documents the results of the Environmental Monitoring Program at the Lawrence Livermore Laboratory (LLNL) for 1987. To evaluate the effect of LLNL operations on the local environment, measurements were made of direct radiation and a variety of radionuclides and chemical pollutants in ambient air, soil, sewage effluents, surface water, groundwater, vegetation, foodstuff, and milk at both the Livermore site and nearby Site 300. Evaluations were made of LLNL's compliance with the applicable guides, standards, and limits for radiological and nonradiological releases to the environment. The data indicates that the only releases in excess of applicable standards were four releases to the sanitary sewer. LLNL operations had no adverse impact on the environment during 1987. 65 refs., 24 figs

  8. Large-scale automation of the Lawrence Livermore Laboratory x-ray analytical facilities

    International Nuclear Information System (INIS)

    Wallace, P.L.; Shimamoto, F.Y.; Quick, T.M.

    1980-01-01

    Lawrence Livermore Laboratory (LLL) has undertaken an ambitious plan to automate its x-ray analytical equipment. This project ultimately will automate 15 x-ray diffraction and 3 x-ray spectrometric systems. All automation is being done by retrofitting existing equipment and combining it with minicomputers to produce smart instruments. Two types of smart instruments have been developed: one that controls an experiment and acquires data and another that analyzes data and communicates with LLL's large computer center. Three of the former type have been built and are operating; seven more will soon be put into service. Only two of the later type are needed, and both are currently in service. We describe the details of our overall plan, the smart instruments, the retrofitting, our current status, and our software

  9. 2002 Small Mammal Inventory at Lawrence Livermore National Laboratory, Site 300

    Energy Technology Data Exchange (ETDEWEB)

    West, E; Woollett, J

    2004-11-16

    To assist the University of California in obtaining biological assessment information for the ''2004 Environmental Impact Statement for Continued Operation of Lawrence Livermore National Laboratory (LLNL)'', Jones & Stokes conducted an inventory of small mammals in six major vegetation communities at Site 300. These communities were annual grassland, native grassland, oak savanna, riparian corridor, coastal scrub, and seep/spring wetlands. The principal objective of this study was to assess the diversity and abundance of small mammal species in these communities, as well as the current status of any special-status small mammal species found in these communities. Surveys in the native grassland community were conducted before and after a controlled fire management burn of the grasslands to qualitatively evaluate any potential effects of fire on small mammals in the area.

  10. Hazardous waste site assessment: Inactive landfill, Site 300, Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    1985-01-01

    This report presents the results of an investigation of an inactive landfill (Pit 6) at Lawrence Livermore National Laboratory's (LLNL) Site 300. The primary objectives were to: collect and review background information pertaining to past waste disposal practices and previous environmental characterization studies; conduct a geophysical survey of the landfill area to locate the buried wastes; conduct a hydrogeologic investigation to provide additional data on the rate and direction of groundwater flow, the extent of any groundwater contamination, and to investigate the connection, if any, of the shallow groundwater beneath the landfill with the local drinking water supply; conduct a risk assessment to identify the degree of threat posed by the landfill to the public health and environment; compile a preliminary list of feasible long-term remedial action alternatives for the landfill; and develop a list of recommendations for any interim measures necessary at the landfill should the long-term remedial action plan be needed.

  11. Construction and operation of replacement hazardous waste handling facility at Lawrence Berkeley Laboratory. Environmental Assessment

    Energy Technology Data Exchange (ETDEWEB)

    1992-09-01

    The US Department of Energy (DOE) has prepared an environmental assessment (EA), DOE/EA-0423, for the construction and operation of a replacement hazardous waste handling facility (HWHF) and decontamination of the existing HWHF at Lawrence Berkeley Laboratory (LBL), Berkeley, California. The proposed facility would replace several older buildings and cargo containers currently being used for waste handling activities and consolidate the LBL`s existing waste handling activities in one location. The nature of the waste handling activities and the waste volume and characteristics would not change as a result of construction of the new facility. Based on the analysis in the EA, DOE has determined that the proposed action would not constitute a major Federal action significantly affecting the quality of the human environment within the meaning of the National Environmental Policy Act (NEPA) of 1969, 42 USC. 4321 et seq. Therefore, an environmental impact statement is not required.

  12. Hazardous waste site assessment: Inactive landfill, Site 300, Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    1985-01-01

    This report presents the results of an investigation of an inactive landfill (Pit 6) at Lawrence Livermore National Laboratory's (LLNL) Site 300. The primary objectives were to: collect and review background information pertaining to past waste disposal practices and previous environmental characterization studies; conduct a geophysical survey of the landfill area to locate the buried wastes; conduct a hydrogeologic investigation to provide additional data on the rate and direction of groundwater flow, the extent of any groundwater contamination, and to investigate the connection, if any, of the shallow groundwater beneath the landfill with the local drinking water supply; conduct a risk assessment to identify the degree of threat posed by the landfill to the public health and environment; compile a preliminary list of feasible long-term remedial action alternatives for the landfill; and develop a list of recommendations for any interim measures necessary at the landfill should the long-term remedial action plan be needed

  13. Federal Facility Compliance Act: Conceptual Site Treatment Plan for Lawrence Livermore National Laboratory, Livermore, California

    International Nuclear Information System (INIS)

    1993-10-01

    The Department of Energy (DOE) is required by section 3021(b) of the Resource Conservation and Recovery Act (RCRA), as amended by the Federal Facility Compliance Act (the Act), to prepare plans describing the development of treatment capacities and technologies for treating mixed waste. The Act requires site treatment plans (STPs or plans) to be developed for each site at which DOE generates or stores mixed waste and submitted to the State or EPA for approval, approval with modification, or disapproval. The Lawrence Livermore National Laboratory (LLNL) Conceptual Site Treatment Plan (CSTP) is the preliminary version of the plan required by the Act and is being provided to California, the US Environmental Protection Agency (EPA), and others for review. A list of the other DOE sites preparing CSTPs is included in Appendix 1.1 of this document. Please note that Appendix 1.1 appears as Appendix A, pages A-1 and A-2 in this document

  14. Contingency plan for the Lawrence Livermore National Laboratory's hazardous-waste operations

    International Nuclear Information System (INIS)

    Roberts, R.S.

    1981-01-01

    The Lawrence Livermore National Laboratory (LLNL) has the necessary equipment and trained personnel to respond to a large number of hazardous material spills and fires or other emergencies resulting from these spills including injured personnel. This response capability is further expanded by the agreements that LLNL has with a number of outside response agencies. The Hazards Control Department at LLNL functions as the central point for coordinating the response of the equipment and personnel. Emergencies involving hazardous waste are also coordinated through the Hazards Control Department, but the equipment and personnel in the Toxic Waste Control Group would be activated for large volume waste pumpouts. Descriptions of response equipment, hazardous waste locations communication systems, and procedures for personnel involved in the emergency are provided

  15. Environmental site characterization and remediation at Lawrence Livermore National Laboratory Site 300

    International Nuclear Information System (INIS)

    Lamarre, A.L.; Ferry, R.A.

    1992-04-01

    Lawrence Livermore National Laboratory (LLNL) is a research and development laboratory owned by the US Department of Energy (DOE) and operated by the University of California. The Laboratory operates its Site 300 test facility in support of DOE's national defense programs. In support of activities, at the 300 Site numerous industrial fluids are used and various process or rinse waters and solid wastes are produced. Some of these materials are hazardous by current standards. HE rinse waters were previously discharged to inlined lagoons; they now are discharged to a permitted Class II surface impoundment Solid wastes have been deposited in nine landfills. Waste HE compounds are destroyed by open burning at a burn pit facility. As a result of these practices, environmental contaminants have been released to the soil and ground water

  16. Computer-aided mapping of stream channels beneath the Lawrence Livermore National Laboratory Super Fund Site

    Energy Technology Data Exchange (ETDEWEB)

    Sick, M. [Lawrence Livermore National Lab., CA (United States)

    1994-12-01

    The Lawrence Livermore National Laboratory (LLNL) site rests upon 300-400 feet of highly heterogeneous braided stream sediments which have been contaminated by a plume of Volatile Organic Compounds (VOCs). The stream channels are filled with highly permeable coarse grained materials that provide quick avenues for contaminant transport. The plume of VOCs has migrated off site in the TFA area, making it the area of greatest concern. I mapped the paleo-stream channels in the TFA area using SLICE an LLNL Auto-CADD routine. SLICE constructed 2D cross sections and sub-horizontal views of chemical, geophysical, and lithologic data sets. I interpreted these 2D views as a braided stream environment, delineating the edges of stream channels. The interpretations were extracted from Auto-CADD and placed into Earth Vision`s 3D modeling and viewing routines. Several 3D correlations have been generated, but no model has yet been chosen as a best fit.

  17. Construction quality assurance closure report, Lawrence Livermore National Laboratory Site 300, Pits 1 and 7

    International Nuclear Information System (INIS)

    1993-02-01

    This document presents the Final Construction Quality Assurance (CQA) report for the closure cover system of two mixed, low-level radioactive and hazardous waste landfills (pits) at Site 300. Site 300, operated by the Lawrence Livermore National Laboratory (LLNL), is located in the Altamont Hills, approximately 15 miles southeast of Livermore, California. The purpose of this report is to document the CQA program established to assure that construction is completed in accordance with the design intent and the approved Closure and Post Closure Plans dated May 1989 and revised January 1990 (EPA ID Number: CA 2890090002). Inclusive within the Closure and Post Closure Plan were the CQA Plan and the Technical Specifications for the final cover system. This report contains a complete narrative with photographic documentation of the construction activities and progress, problems encountered and solutions utilized, and third party testing and monitoring results, thus establishing the verification of compliance with the Quality Assurance Plan for the project

  18. Remedial investigation of the High-Explosives (HE) Process Area, Lawrence Livermore National Laboratory Site 300

    Energy Technology Data Exchange (ETDEWEB)

    Crow, N.B.; Lamarre, A.L.

    1990-08-01

    This report presents the results of a Remedial Investigation (RI) to define the extent of high explosives (HE) compounds and volatile organic compounds (VOCs) found in the soil, rocks, and ground water of the HE Process Area of Lawrence Livermore National Laboratory's (LLNL) Site 300 Facility. The report evaluates potential public health environmental risks associated with these compounds. Hydrogeologic information available before February 15, 1990, is included; however, chemical analyses and water-level data are reported through March 1990. This report is intended to assist the California Regional Water Quality Control Board (RWQCB)--Central Valley Region and the US Environmental Protection Agency (EPA) in evaluating the extent of environmental contamination of the LLNL HE Process Area and ultimately in designing remedial actions. 90 refs., 20 figs., 7 tabs.

  19. High Energy, Short Pulse Fiber Injection Lasers at Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Dawson, J W; Messerly, M J; Phan, H H; Crane, J K; Beach, R J; Siders, C W; Barty, C J

    2008-09-10

    A short pulse fiber injection laser for the Advanced Radiographic Capability (ARC) on the National Ignition Facility (NIF) has been developed at Lawrence Livermore National Laboratory (LLNL). This system produces 100 {micro}J pulses with 5 nm of bandwidth centered at 1053 nm. The pulses are stretched to 2.5 ns and have been recompressed to sub-ps pulse widths. A key feature of the system is that the pre-pulse power contrast ratio exceeds 80 dB. The system can also precisely adjust the final recompressed pulse width and timing and has been designed for reliable, hands free operation. The key challenges in constructing this system were control of the signal to noise ratio, dispersion management and managing the impact of self phase modulation on the chirped pulse.

  20. Hazardous Waste Cerification Plan: Hazardous Waste Handling Facility, Lawrence Berkeley Laboratory

    International Nuclear Information System (INIS)

    1992-02-01

    The purpose of this plan is to describe the organization and methodology for the certification of hazardous waste (HW) handled in the Lawrence Berkeley Laboratory (LBL) Hazardous Waste Handling Facility (HWHF). The plan also incorporates the applicable elements of waste reduction, which include both up-front minimization and end- product treatment to reduce the volume and toxicity of the waste; segregation of the waste as it applies to certification; and executive summary of the Quality Assurance Program Plan (QAPP) for the HWHF and a list of the current and planned implementing procedures used in waste certification. The plan provides guidance from the HWHF to waste generators, waste handlers, and the Systems Group Manager to enable them to conduct their activities and carry out their responsibilities in a manner that complies with several requirements of the Federal Resource Conservation and Resource Recovery Act (RCRA), the Federal Department of Transportation (DOT), and the State of California, Code of Regulations (CCR), Title 22

  1. miR-132 and miR-212 are increased in pancreatic cancer and target the retinoblastoma tumor suppressor

    Energy Technology Data Exchange (ETDEWEB)

    Park, Jong-Kook [College of Pharmacy, Ohio State University, Columbus, OH 43210 (United States); Henry, Jon C. [Department of Surgery, Ohio State University, Columbus, OH 43210 (United States); Jiang, Jinmai [College of Pharmacy, Ohio State University, Columbus, OH 43210 (United States); Esau, Christine [Regulus Therapeutics, Carlsbad, CA (United States); Gusev, Yuriy [Lombardi Cancer Center, Georgetown University, Washington, DC (United States); Lerner, Megan R. [Veterans Affairs Medical Center, Oklahoma City, OK (United States); Postier, Russell G. [Department of Surgery, University of Oklahoma Health Sciences Center, Oklahoma City, OK (United States); Brackett, Daniel J. [Veterans Affairs Medical Center, Oklahoma City, OK (United States); Schmittgen, Thomas D., E-mail: Schmittgen.2@osu.edu [College of Pharmacy, Ohio State University, Columbus, OH 43210 (United States)

    2011-03-25

    Research highlights: {yields} The expression of miR-132 and miR-212 are significantly increased in pancreatic cancer. {yields} miR-132 and miR-212 target the tumor suppressor pRb, resulting in enhanced proliferation. {yields} miR-132 and miR-212 expression is increased by a {beta}2 adrenergic receptor agonist, suggesting a novel mechanism for pancreatic cancer progression. -- Abstract: Numerous microRNAs (miRNAs) are reported as differentially expressed in cancer, however the consequence of miRNA deregulation in cancer is unknown for many miRNAs. We report that two miRNAs located on chromosome 17p13, miR-132 and miR-212, are over-expressed in pancreatic adenocarcinoma (PDAC) tissues. Both miRNAs are predicted to target the retinoblastoma tumor suppressor, Rb1. Validation of this interaction was confirmed by luciferase reporter assay and western blot in a pancreatic cancer cell line transfected with pre-miR-212 and pre-miR-132 oligos. Cell proliferation was enhanced in Panc-1 cells transfected with pre-miR-132/-212 oligos. Conversely, antisense oligos to miR-132/-212 reduced cell proliferation and caused a G{sub 2}/M cell cycle arrest. The mRNA of a number of E2F transcriptional targets were increased in cells over expressing miR-132/-212. Exposing Panc-1 cells to the {beta}2 adrenergic receptor agonist, terbutaline, increased the miR-132 and miR-212 expression by 2- to 4-fold. We report that over-expression of miR-132 and miR-212 result in reduced pRb protein in pancreatic cancer cells and that the increase in cell proliferation from over-expression of these miRNAs is likely due to increased expression of several E2F target genes. The {beta}2 adrenergic pathway may play an important role in this novel mechanism.

  2. miR-132 and miR-212 are increased in pancreatic cancer and target the retinoblastoma tumor suppressor

    International Nuclear Information System (INIS)

    Park, Jong-Kook; Henry, Jon C.; Jiang, Jinmai; Esau, Christine; Gusev, Yuriy; Lerner, Megan R.; Postier, Russell G.; Brackett, Daniel J.; Schmittgen, Thomas D.

    2011-01-01

    Research highlights: → The expression of miR-132 and miR-212 are significantly increased in pancreatic cancer. → miR-132 and miR-212 target the tumor suppressor pRb, resulting in enhanced proliferation. → miR-132 and miR-212 expression is increased by a β2 adrenergic receptor agonist, suggesting a novel mechanism for pancreatic cancer progression. -- Abstract: Numerous microRNAs (miRNAs) are reported as differentially expressed in cancer, however the consequence of miRNA deregulation in cancer is unknown for many miRNAs. We report that two miRNAs located on chromosome 17p13, miR-132 and miR-212, are over-expressed in pancreatic adenocarcinoma (PDAC) tissues. Both miRNAs are predicted to target the retinoblastoma tumor suppressor, Rb1. Validation of this interaction was confirmed by luciferase reporter assay and western blot in a pancreatic cancer cell line transfected with pre-miR-212 and pre-miR-132 oligos. Cell proliferation was enhanced in Panc-1 cells transfected with pre-miR-132/-212 oligos. Conversely, antisense oligos to miR-132/-212 reduced cell proliferation and caused a G 2 /M cell cycle arrest. The mRNA of a number of E2F transcriptional targets were increased in cells over expressing miR-132/-212. Exposing Panc-1 cells to the β2 adrenergic receptor agonist, terbutaline, increased the miR-132 and miR-212 expression by 2- to 4-fold. We report that over-expression of miR-132 and miR-212 result in reduced pRb protein in pancreatic cancer cells and that the increase in cell proliferation from over-expression of these miRNAs is likely due to increased expression of several E2F target genes. The β2 adrenergic pathway may play an important role in this novel mechanism.

  3. Decreased expression of miR-21, miR-26a, miR-29a, and miR-142-3p in CD4⁺ T cells and peripheral blood from tuberculosis patients.

    Science.gov (United States)

    Kleinsteuber, Katja; Heesch, Kerrin; Schattling, Stefanie; Kohns, Malte; Sander-Jülch, Claudia; Walzl, Gerhard; Hesseling, Anneke; Mayatepek, Ertan; Fleischer, Bernhard; Marx, Florian M; Jacobsen, Marc

    2013-01-01

    The vast majority of Mycobacterium tuberculosis (M. tuberculosis) infected individuals are protected from developing tuberculosis and T cells are centrally involved in this process. MicroRNAs (miRNA) regulate T-cell functions and are biomarker candidates of disease susceptibility and treatment efficacy in M. tuberculosis infection. We determined the expression profile of 29 selected miRNAs in CD4(+) T cells from tuberculosis patients and contacts with latent M. tuberculosis infection (LTBI). These analyses showed lower expression of miR-21, miR-26a, miR-29a, and miR-142-3p in CD4(+) T cells from tuberculosis patients. Whole blood miRNA candidate analyses verified decreased expression of miR-26a, miR-29a, and miR-142-3p in children with tuberculosis as compared to healthy children with LTBI. Despite marked variances between individual donor samples, trends of increased miRNA candidate expression during treatment and recovery were observed. Functional in vitro analysis identified increased miR-21 and decreased miR-26a expression after re-stimulation of T cells. In vitro polarized Interleukin-17 positive T-cell clones showed activation-dependent miR-29a up-regulation. In order to characterize the role of miR-29a (a described suppressor of Interferon-γ in tuberculosis), we analyzed M. tuberculosis specific Interferon-γ expressing T cells in children with tuberculosis and healthy contacts but detected no correlation between miR-29a and Interferon-γ expression. Suppression of miR-29a in primary human T cells by antagomirs indicated no effect on Interferon-γ expression after in vitro activation. Finally, classification of miRNA targets revealed only a moderate overlap between the candidates. This may reflect differential roles of miR-21, miR-26a, miR-29a, and miR-142-3p in T-cell immunity against M. tuberculosis infection and disease.

  4. Decreased Expression of miR-21, miR-26a, miR-29a, and miR-142-3p in CD4+ T Cells and Peripheral Blood from Tuberculosis Patients

    Science.gov (United States)

    Schattling, Stefanie; Kohns, Malte; Sander-Jülch, Claudia; Walzl, Gerhard; Hesseling, Anneke; Mayatepek, Ertan; Fleischer, Bernhard; Marx, Florian M.; Jacobsen, Marc

    2013-01-01

    The vast majority of Mycobacterium tuberculosis (M. tuberculosis) infected individuals are protected from developing tuberculosis and T cells are centrally involved in this process. MicroRNAs (miRNA) regulate T-cell functions and are biomarker candidates of disease susceptibility and treatment efficacy in M. tuberculosis infection. We determined the expression profile of 29 selected miRNAs in CD4+ T cells from tuberculosis patients and contacts with latent M. tuberculosis infection (LTBI). These analyses showed lower expression of miR-21, miR-26a, miR-29a, and miR-142-3p in CD4+ T cells from tuberculosis patients. Whole blood miRNA candidate analyses verified decreased expression of miR-26a, miR-29a, and miR-142-3p in children with tuberculosis as compared to healthy children with LTBI. Despite marked variances between individual donor samples, trends of increased miRNA candidate expression during treatment and recovery were observed. Functional in vitro analysis identified increased miR-21 and decreased miR-26a expression after re-stimulation of T cells. In vitro polarized Interleukin-17 positive T-cell clones showed activation-dependent miR-29a up-regulation. In order to characterize the role of miR-29a (a described suppressor of Interferon-γ in tuberculosis), we analyzed M. tuberculosis specific Interferon-γ expressing T cells in children with tuberculosis and healthy contacts but detected no correlation between miR-29a and Interferon-γ expression. Suppression of miR-29a in primary human T cells by antagomirs indicated no effect on Interferon-γ expression after in vitro activation. Finally, classification of miRNA targets revealed only a moderate overlap between the candidates. This may reflect differential roles of miR-21, miR-26a, miR-29a, and miR-142-3p in T-cell immunity against M. tuberculosis infection and disease. PMID:23613882

  5. Feeding by grey seals in the Gulf of St. Lawrence and around Newfoundland

    Directory of Open Access Journals (Sweden)

    M O Hammill

    2007-01-01

    Full Text Available Diet composition of grey seals in the Gulf of St. Lawrence (Gulf and around the coast of Newfoundland, Canada, was examined using identification of otoliths recovered from digestive tracts. Prey were recovered from 632 animals. Twenty-nine different prey taxa were identified. Grey seals sampled in the northern Gulf of St. Lawrence fed mainly on capelin, mackerel, wolffish and lumpfish during the spring, but consumed more cod, sandlance and winter flounder during late summer. Overall, the southern Gulf diet was more diverse, with sandlance, Atlantic cod, cunner, white hake and Atlantic herring dominating the diet. Capelin and winter flounder were the dominant prey in grey seals sampled from the east coast of Newfoundland, while Atlantic cod, flatfish and capelin were the most important prey from the south coast. Animals consumed prey with an average length of 20.4 cm (Range 4.2-99.2 cm. Capelin were the shortest prey (Mean = 13.9 cm, SE = 0.08, N = 1126, while wolffish were the longest with the largest fish having an estimated length of 99.2 cm (Mean = 59.4, SE = 2.8, N = 63. In the early 1990s most cod fisheries in Atlantic Canada were closed because of the collapse of the stocks. Since then they have shown limited sign of recovery. Diet samples from the west coast of Newfoundland indicate a decline in the contribution of cod to the diet from the pre-collapse to the postcollapse period, while samples from the southern Gulf indicate little change in the contribution of cod.

  6. Environmental management assessment of the Lawrence Livermore National Laboratory Livermore, California

    International Nuclear Information System (INIS)

    1994-06-01

    This report documents the results of the Environmental Management Assessment performed at the Lawrence Livermore National Laboratory (LLNL), Livermore, CA. LLNL is operated by the University of California (UC) under contract with the U.S. Department of Energy (DOE). Major programs at LLNL include research, development, and test activities associated with the nuclear design aspects of the nuclear weapons life cycle and related national security tasks; inertial confinement fusion; magnetic fusion energy; biomedical and environmental research; laser isotope separation; energy-related research; beam research physics; and support to a variety of Defense and other Federal agencies. During this assessment, activities and records were reviewed and interviews were conducted with personnel from management and operating contractor, Lawrence Livermore National Laboratory; DOE Oakland Operations Office; and DOE Headquarters Program Offices, including the Office of Defense Programs, Office of Environmental Management, the Office of Nuclear Energy, and the Office of Energy Research. The onsite portion was conducted in June 1994, by the DOE Office of Environmental Audit. The goal of EH-24 is enhancement of environmental protection and minimization of risk to public health and the environment. EH-24 accomplishes its mission using systematic and periodic evaluations of DOE's environmental programs within line organizations, and through use of supplemental activities that strengthen self-assessment and oversight functions within program, field, and contractor organizations. The Environmental Management Assessment of LLNL revealed that LLNL's environmental program is exemplary within the DOE complex and that all levels of LLNL management and staff consistently exhibit a high level of commitment to achieve environmental excellence

  7. LBNE-NuMI Muon Detectors

    Energy Technology Data Exchange (ETDEWEB)

    Andrews, Mike [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); Mills, Geoffrey [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); Marino, Alysia [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); Zimmerman, Eric [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); Lane, Charles [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); Bern, Hans [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States)

    2013-08-15

    This is a technical scope of work (TSW) that concerns Fermi National Laboratory and the experiments of LBNE who have committed to participate in muon detector prototype tests to be carried out in the NuMi alcoves during the 2013-2017 Fermilab Neutrino program.

  8. miRNAs: Small but deadly

    African Journals Online (AJOL)

    Jane

    2011-08-24

    Aug 24, 2011 ... Levels of some miRNAs are found altered in cancers, so we might expect these regulatory ..... males is the prostate cancer (PCa) (Jemal et al., 2008). ..... 1 growth factor receptor family members HER-1, HER-2, and HER-3.

  9. Saginaw Bay, MI LiDAR

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — TASK NAME:(NRCS) Saginaw Bay, MI LiDAR LiDAR Data Acquisition and Processing Production Task USGS Contract No. G10PC00057 Task Order No. G11PD01254 Woolpert Order...

  10. miR-21 Is Linked to Glioma Angiogenesis

    DEFF Research Database (Denmark)

    Hermansen, Simon Kjær; Nielsen, Boye Schnack; Aaberg-Jessen, Charlotte

    2016-01-01

    MicroRNA-21 (miR-21) is the most consistently over-expressed microRNA (miRNA) in malignant gliomas. We have previously reported that miR-21 is upregulated in glioma vessels and subsets of glioma cells. To better understand the role of miR-21 in glioma angiogenesis and to characterize miR-21......-localized with the hypoxia- and angiogenesis-associated markers HIF-1α (p=0.0020) and VEGF (p=0.0096), whereas the putative miR-21 target, PTEN, was expressed independently of miR-21. Expression of stem cell markers Oct4, Sox2 and CD133 was not associated with miR-21. In six glioblastoma cultures, miR-21 did not correlate...... with the six markers. These findings suggest that miR-21 is linked to glioma angiogenesis, that miR-21 is unlikely to regulate PTEN, and that miR-21-positive tumor cells do not possess stem cell characteristics....

  11. Protein-driven inference of miRNA-disease associations

    DEFF Research Database (Denmark)

    Mørk, Søren; Pletscher-Frankild, Sune; Palleja, Albert

    2014-01-01

    MicroRNAs (miRNAs) are a highly abundant class of non-coding RNA genes involved in cellular regulation and thus also diseases. Despite miRNAs being important disease factors, miRNA-disease associations remain low in number and of variable reliability. Furthermore, existing databases and prediction...

  12. miR319, miR390, and miR393 Are Involved in Aluminum Response in Flax (Linum usitatissimum L.).

    Science.gov (United States)

    Dmitriev, Alexey A; Kudryavtseva, Anna V; Bolsheva, Nadezhda L; Zyablitsin, Alexander V; Rozhmina, Tatiana A; Kishlyan, Natalya V; Krasnov, George S; Speranskaya, Anna S; Krinitsina, Anastasia A; Sadritdinova, Asiya F; Snezhkina, Anastasiya V; Fedorova, Maria S; Yurkevich, Olga Yu; Muravenko, Olga V; Belenikin, Maxim S; Melnikova, Nataliya V

    2017-01-01

    Acid soils limit agricultural production worldwide. Major reason of crop losses in acid soils is the toxicity of aluminum (Al). In the present work, we investigated expression alterations of microRNAs in flax ( Linum usitatissimum L.) plants under Al stress. Flax seedlings of resistant (TMP1919 and G1071/4_k) and sensitive (Lira and G1071/4_o) to Al cultivars and lines were exposed to AlCl 3 solution for 4 and 24 hours. Twelve small RNA libraries were constructed and sequenced using Illumina platform. In total, 97 microRNAs from 18 conserved families were identified. miR319, miR390, and miR393 revealed expression alterations associated with Al treatment of flax plants. Moreover, for miR390 and miR393, the alterations were distinct in sensitive and resistant to Al genotypes. Expression level changes of miR319 and miR390 were confirmed using qPCR analysis. In flax, potential targets of miR319 are TCPs, miR390-TAS3 and GRF5, and miR393-AFB2-coding transcripts. TCPs, TAS3, GRF5, and AFB2 participate in regulation of plant growth and development. The involvement of miR319, miR390, and miR393 in response to Al stress in flax was shown here for the first time. We speculate that these microRNAs play an important role in Al response via regulation of growth processes in flax plants.

  13. 33 CFR 207.610 - St. Lawrence River, Cape Vincent Harbor, N.Y.; use, administration, and navigation of the harbor...

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false St. Lawrence River, Cape Vincent Harbor, N.Y.; use, administration, and navigation of the harbor and U.S. breakwater. 207.610 Section 207... NAVIGATION REGULATIONS § 207.610 St. Lawrence River, Cape Vincent Harbor, N.Y.; use, administration, and...

  14. Lawrence Lessig, The architecture of access to scientific knowledge: just how badly we have messed this up

    Directory of Open Access Journals (Sweden)

    Maria Chiara Pievatolo

    2011-06-01

    Full Text Available E’ una conferenza tenuta da Lawrence Lessig al Cern di Ginevra il 18 aprile di quest’anno. Il suo video è disponibile presso il Cern Document Server; a partire da qui si possono trovare i sottotitoli in inglese e in altre lingue, compresa quella italiana. Lessig esordisce proponendo due impressioni. La prima è un effetto chiamato [...

  15. 75 FR 1010 - CSX Transportation, Inc.-Discontinuance of Service Exemption-in Clark, Floyd, Lawrence, Orange...

    Science.gov (United States)

    2010-01-07

    ... DEPARTMENT OF TRANSPORTATION Surface Transportation Board [STB Docket No. AB-55 (Sub-No. 698X)] CSX Transportation, Inc.--Discontinuance of Service Exemption--in Clark, Floyd, Lawrence, Orange, and..., Orange, and Washington Counties, IN.\\1\\ The line traverses United States Postal Service Zip Codes 47150...

  16. A practical MRI grading system for osteoarthritis of the knee: Association with Kellgren–Lawrence radiographic scores

    Energy Technology Data Exchange (ETDEWEB)

    Park, Hee-Jin, E-mail: parkhiji@gmail.com [Department of Radiology, Sungkyunkwan University School of Medicine, Kangbuk Samsung Hospital, #108 Pyung-dong, Jongno-gu, Seoul 110-746 (Korea, Republic of); Department of Radiology, Kangwon National University School of Medicine, Baengnyeong-ro 156, Chuncheon-Si, Gangwon-Do Kangwon National University Hospital 200-722 (Korea, Republic of); Kim, Sam Soo, E-mail: samskim@kangwon.ac.kr [Department of Radiology, Kangwon National University School of Medicine, Baengnyeong-ro 156, Chuncheon-Si, Gangwon-Do Kangwon National University Hospital 200-722 (Korea, Republic of); Lee, So-Yeon, E-mail: parkhiji@kwandong.ac.kr [Department of Radiology, Sungkyunkwan University School of Medicine, Kangbuk Samsung Hospital, #108 Pyung-dong, Jongno-gu, Seoul 110-746 (Korea, Republic of); Park, Noh-Hyuck, E-mail: nhpark904@kwandong.ac.kr [Department of Radiology, Myongji Hospital, Kwandong University, College of Medicine, 697-24 Hwajung-dong, Dukyang-ku, Koyang, Kyunggi 412-270 (Korea, Republic of); Park, Ji-Yeon, E-mail: zzzz3@hanmail.net [Department of Radiology, Myongji Hospital, Kwandong University, College of Medicine, 697-24 Hwajung-dong, Dukyang-ku, Koyang, Kyunggi 412-270 (Korea, Republic of); Choi, Yoon-Jung, E-mail: yoonchoi99@gmail.com [Department of Radiology, Sungkyunkwan University School of Medicine, Kangbuk Samsung Hospital, #108 Pyung-dong, Jongno-gu, Seoul 110-746 (Korea, Republic of); Jeon, Hyun-Jun, E-mail: ostrich-13@hanmail.net [Department of Occupational Medicine, Dongsan Medical Center, Keimyung University School of Medicine, 194 Dongsan-Dong, Jung-ku, Taegu (Korea, Republic of)

    2013-01-15

    Purpose: To propose a reproducible and constant MR grading system for osteoarthritis of the knee joint that provides high interobserver and intraoberver agreement and that does not require complicated calculation procedures. Materials and methods: This retrospective study sample included 44 men and 65 women who underwent both MRI and plain radiography of the knee at our institution. All patients were older than 50 years of age (mean 57.7) and had clinically suspected osteoarthritis of the knee. The standard of 4 grades on the MR grade scale was based mainly on cartilage injury and additional findings. Kellgren–Lawrence grades were assessed for the same patient group. The relationship between the results was determined. Statistical analyses were performed including kappa statistics, categorical regression analysis and nonparametric correlation analysis. Results: The interobserver and intraoberver agreements between the two readers in the grading of osteoarthritis were found to be almost perfect. Interobserver and intraobserver agreements were slightly lower for the MR grading system than for the Kellgren–Lawrence grading scale. The correlation between the MR grade and Kellgren–Lawrence grade was very high and did not differ with patient age. The MR grades were highly correlated with the Kellgren–Lawrence grades and showed excellent interobserver and intraobserver agreements. Conclusion: This new MR grading system for osteoarthritis of the knee joint is reproducible and may be helpful for the grading of osteoarthritis of the knee without requiring reference to plain radiography.

  17. A practical MRI grading system for osteoarthritis of the knee: Association with Kellgren–Lawrence radiographic scores

    International Nuclear Information System (INIS)

    Park, Hee-Jin; Kim, Sam Soo; Lee, So-Yeon; Park, Noh-Hyuck; Park, Ji-Yeon; Choi, Yoon-Jung; Jeon, Hyun-Jun

    2013-01-01

    Purpose: To propose a reproducible and constant MR grading system for osteoarthritis of the knee joint that provides high interobserver and intraoberver agreement and that does not require complicated calculation procedures. Materials and methods: This retrospective study sample included 44 men and 65 women who underwent both MRI and plain radiography of the knee at our institution. All patients were older than 50 years of age (mean 57.7) and had clinically suspected osteoarthritis of the knee. The standard of 4 grades on the MR grade scale was based mainly on cartilage injury and additional findings. Kellgren–Lawrence grades were assessed for the same patient group. The relationship between the results was determined. Statistical analyses were performed including kappa statistics, categorical regression analysis and nonparametric correlation analysis. Results: The interobserver and intraoberver agreements between the two readers in the grading of osteoarthritis were found to be almost perfect. Interobserver and intraobserver agreements were slightly lower for the MR grading system than for the Kellgren–Lawrence grading scale. The correlation between the MR grade and Kellgren–Lawrence grade was very high and did not differ with patient age. The MR grades were highly correlated with the Kellgren–Lawrence grades and showed excellent interobserver and intraobserver agreements. Conclusion: This new MR grading system for osteoarthritis of the knee joint is reproducible and may be helpful for the grading of osteoarthritis of the knee without requiring reference to plain radiography

  18. Identification of novel miRNAs and miRNA dependent developmental shifts of gene expression in Arabidopsis thaliana.

    Directory of Open Access Journals (Sweden)

    Shuhua Zhan

    Full Text Available microRNAs (miRNAs are small, endogenous RNAs of 20 approximately 25 nucleotides, processed from stem-loop regions of longer RNA precursors. Plant miRNAs act as negative regulators of target mRNAs predominately by slicing target transcripts, and a number of miRNAs play important roles in development. We analyzed a number of published datasets from Arabidopsis thaliana to characterize novel miRNAs, novel miRNA targets, and miRNA-regulated developmental changes in gene expression. These data include microarray profiling data and small RNA (sRNA deep sequencing data derived from miRNA biogenesis/transport mutants, microarray profiling data of mRNAs in a developmental series, and computational predictions of conserved genomic stem-loop structures. Our conservative analyses identified five novel mature miRNAs and seven miRNA targets, including one novel target gene. Two complementary miRNAs that target distinct mRNAs were encoded by one gene. We found that genes targeted by known miRNAs, and genes up-regulated or down-regulated in miRNA mutant inflorescences, are highly expressed in the wild type inflorescence. In addition, transcripts upregulated within the mutant inflorescences were abundant in wild type leaves and shoot meristems and low in pollen and seed. Downregulated transcripts were abundant in wild type pollen and seed and low in shoot meristems, roots and leaves. Thus, disrupting miRNA function causes the inflorescence transcriptome to resemble the leaf and meristem and to differ from pollen and seed. Applications of our computational approach to other species and the use of more liberal criteria than reported here will further expand the number of identified miRNAs and miRNA targets. Our findings suggest that miRNAs have a global role in promoting vegetative to reproductive transitions in A. thaliana.

  19. Cloning and characterization of pre-miR159a and pre-miR1123 from ...

    African Journals Online (AJOL)

    Although many miRNA genes are conserved across the plant species, the same gene family varies significantly in size and genomic organization in different species. ... Sequence identity matrix suggests 43-82% variation in precursor of Tae AL pre-miR159a (Tae Agra local pre-miR159a) across the species. On the other ...

  20. miR-10 in development and cancer

    DEFF Research Database (Denmark)

    Lund, Anders Henrik

    2010-01-01

    The microRNA (miRNA) miR-10 family has attracted attention because of its conservation and the position of the miR-10 genes within the Hox clusters of developmental regulators. In several species, miR-10 is coexpressed with a set of Hox genes and has been found to regulate the translation of Hox ...... function to the miRNA repertoire.Cell Death and Differentiation advance online publication, 22 May 2009; doi:10.1038/cdd.2009.58....

  1. Epigenetic architecture and miRNA: reciprocal regulators

    DEFF Research Database (Denmark)

    Wiklund, Erik D; Kjems, Jørgen; Clark, Susan J

    2010-01-01

    Deregulation of epigenetic and microRNA (miRNA) pathways are emerging as key events in carcinogenesis. miRNA genes can be epigenetically regulated and miRNAs can themselves repress key enzymes that drive epigenetic remodeling. Epigenetic and miRNA functions are thus tightly interconnected......RNAs) are considered especially promising in clinical applications, and their biogenesis and function is a subject of active research. In this review, the current status of epigenetic miRNA regulation is summarized and future therapeutic prospects in the field are discussed with a focus on cancer....

  2. Analysis of the hydraulic data from the MI fracture zone at the Grimsel Rock Laboratory, Switzerland

    International Nuclear Information System (INIS)

    Davey, A.; Karasaki, K.; Long, J.C.S.; Landsfeld, M.; Mensch, A.; Martel, S.J.

    1989-10-01

    One of the major problems in analyzing flow and transport in fractured rock is that the flow may be largely confined to a poorly connected network of fractures. In order to overcome some of this problem, Lawrence Berkeley Laboratory (LBL) has been developing a new type of fracture hydrology model called an equivalent discontinuum model. In this model the authors represent the discontinuous nature of the problem through flow on a partially filled lattice. A key component in constructing an equivalent discontinuum model from this lattice is removing some of the conductive elements such that the system is partially connected in the same manner as the fracture network. This is done through a statistical inverse technique called simulated annealing. The fracture network model is annealed by continually modifying a base model, or template such that the modified systems behave more and more like the observed system. In order to see how the simulated annealing algorithm works, the authors have developed a series of synthetic real cases. In these cases, the real system is completely known so that the results of annealing to steady state data can be evaluated absolutely. The effect of the starting configuration has been studied by varying the percent of conducting elements in the initial configuration. Results have shown that the final configurations converge to about the same percentage of conducting elements. An example using Nagra field data from the Migration Experiment (MI) at Grimsel Rock Laboratory in Switzerland is also analyzed. 24 refs., 33 figs., 3 tabs

  3. Exploration of miRNA families for hypotheses generation.

    KAUST Repository

    Kamanu, T.K.

    2013-10-15

    Technological improvements have resulted in increased discovery of new microRNAs (miRNAs) and refinement and enrichment of existing miRNA families. miRNA families are important because they suggest a common sequence or structure configuration in sets of genes that hint to a shared function. Exploratory tools to enhance investigation of characteristics of miRNA families and the functions of family-specific miRNA genes are lacking. We have developed, miRNAVISA, a user-friendly web-based tool that allows customized interrogation and comparisons of miRNA families for hypotheses generation, and comparison of per-species chromosomal distribution of miRNA genes in different families. This study illustrates hypothesis generation using miRNAVISA in seven species. Our results unveil a subclass of miRNAs that may be regulated by genomic imprinting, and also suggest that some miRNA families may be species-specific, as well as chromosome- and/or strand-specific.

  4. Entropy-based model for miRNA isoform analysis.

    Directory of Open Access Journals (Sweden)

    Shengqin Wang

    Full Text Available MiRNAs have been widely studied due to their important post-transcriptional regulatory roles in gene expression. Many reports have demonstrated the evidence of miRNA isoform products (isomiRs in high-throughput small RNA sequencing data. However, the biological function involved in these molecules is still not well investigated. Here, we developed a Shannon entropy-based model to estimate isomiR expression profiles of high-throughput small RNA sequencing data extracted from miRBase webserver. By using the Kolmogorov-Smirnov statistical test (KS test, we demonstrated that the 5p and 3p miRNAs present more variants than the single arm miRNAs. We also found that the isomiR variant, except the 3' isomiR variant, is strongly correlated with Minimum Free Energy (MFE of pre-miRNA, suggesting the intrinsic feature of pre-miRNA should be one of the important factors for the miRNA regulation. The functional enrichment analysis showed that the miRNAs with high variation, particularly the 5' end variation, are enriched in a set of critical functions, supporting these molecules should not be randomly produced. Our results provide a probabilistic framework for miRNA isoforms analysis, and give functional insights into pre-miRNA processing.

  5. Evolutionary relationships between miRNA genes and their activity.

    Science.gov (United States)

    Zhu, Yan; Skogerbø, Geir; Ning, Qianqian; Wang, Zhen; Li, Biqing; Yang, Shuang; Sun, Hong; Li, Yixue

    2012-12-22

    The emergence of vertebrates is characterized by a strong increase in miRNA families. MicroRNAs interact broadly with many transcripts, and the evolution of such a system is intriguing. However, evolutionary questions concerning the origin of miRNA genes and their subsequent evolution remain unexplained. In order to systematically understand the evolutionary relationship between miRNAs gene and their function, we classified human known miRNAs into eight groups based on their evolutionary ages estimated by maximum parsimony method. New miRNA genes with new functional sequences accumulated more dynamically in vertebrates than that observed in Drosophila. Different levels of evolutionary selection were observed over miRNA gene sequences with different time of origin. Most genic miRNAs differ from their host genes in time of origin, there is no particular relationship between the age of a miRNA and the age of its host genes, genic miRNAs are mostly younger than the corresponding host genes. MicroRNAs originated over different time-scales are often predicted/verified to target the same or overlapping sets of genes, opening the possibility of substantial functional redundancy among miRNAs of different ages. Higher degree of tissue specificity and lower expression level was found in young miRNAs. Our data showed that compared with protein coding genes, miRNA genes are more dynamic in terms of emergence and decay. Evolution patterns are quite different between miRNAs of different ages. MicroRNAs activity is under tight control with well-regulated expression increased and targeting decreased over time. Our work calls attention to the study of miRNA activity with a consideration of their origin time.

  6. Role of miRNA-9 in Brain Development

    Directory of Open Access Journals (Sweden)

    Balachandar Radhakrishnan

    2016-01-01

    Full Text Available MicroRNAs (miRNAs are a class of small regulatory RNAs involved in gene regulation. The regulation is effected by either translational inhibition or transcriptional silencing. In vertebrates, the importance of miRNA in development was discovered from mice and zebrafish dicer knockouts. The miRNA-9 (miR-9 is one of the most highly expressed miRNAs in the early and adult vertebrate brain. It has diverse functions within the developing vertebrate brain. In this article, the role of miR-9 in the developing forebrain (telencephalon and diencephalon, midbrain, hindbrain, and spinal cord of vertebrate species is highlighted. In the forebrain, miR-9 is necessary for the proper development of dorsoventral telencephalon by targeting marker genes expressed in the telencephalon. It regulates proliferation in telencephalon by regulating Foxg1, Pax6, Gsh2 , and Meis2 genes. The feedback loop regulation between miR-9 and Nr2e1/Tlx helps in neuronal migration and differentiation. Targeting Foxp1 and Foxp2 , and Map1b by miR-9 regulates the radial migration of neurons and axonal development. In the organizers, miR-9 is inversely regulated by hairy1 and Fgf8 to maintain zona limitans interthalamica and midbrain-hindbrain boundary (MHB. It maintains the MHB by inhibiting Fgf signaling genes and is involved in the neurogenesis of the midbrain-hindbrain by regulating Her genes. In the hindbrain, miR-9 modulates progenitor proliferation and differentiation by regulating Her genes and Elav3. In the spinal cord, miR-9 modulates the regulation of Foxp1 and Onecut1 for motor neuron development. In the forebrain, midbrain, and hindbrain, miR-9 is necessary for proper neuronal progenitor maintenance, neurogenesis, and differentiation. In vertebrate brain development, miR-9 is involved in regulating several region-specific genes in a spatiotemporal pattern.

  7. Prognostic significance of miR-205 in endometrial cancer.

    Directory of Open Access Journals (Sweden)

    Mihriban Karaayvaz

    Full Text Available microRNAs have emerged as key regulators of gene expression, and their altered expression has been associated with tumorigenesis and tumor progression. Thus, microRNAs have potential as both cancer biomarkers and/or potential novel therapeutic targets. Although accumulating evidence suggests the role of aberrant microRNA expression in endometrial carcinogenesis, there are still limited data available about the prognostic significance of microRNAs in endometrial cancer. The goal of this study is to investigate the prognostic value of selected key microRNAs in endometrial cancer by the analysis of archival formalin-fixed paraffin-embedded tissues.Total RNAs were extracted from 48 paired normal and endometrial tumor specimens using Trizol based approach. The expression of miR-26a, let-7g, miR-21, miR-181b, miR-200c, miR-192, miR-215, miR-200c, and miR-205 were quantified by real time qRT-PCR expression analysis. Targets of the differentially expressed miRNAs were quantified using immunohistochemistry. Statistical analysis was performed by GraphPad Prism 5.0.The expression levels of miR-200c (P<0.0001 and miR-205 (P<0.0001 were significantly increased in endometrial tumors compared to normal tissues. Kaplan-Meier survival analysis revealed that high levels of miR-205 expression were associated with poor patient overall survival (hazard ratio, 0.377; Logrank test, P = 0.028. Furthermore, decreased expression of a miR-205 target PTEN was detected in endometrial cancer tissues compared to normal tissues.miR-205 holds a unique potential as a prognostic biomarker in endometrial cancer.

  8. Numerical analysis of temperature distribution due to basement radiogenic heat production, St. Lawrence Lowlands, eastern Canada

    Science.gov (United States)

    Liu, Hejuan; Giroux, Bernard; Harris, Lyal B.; Mansour, John

    2017-04-01

    Although eastern Canada is considered as having a low potential for high-temperature geothermal resources, the possibility for additional localized radioactive heat sources in Mesoproterozoic Grenvillian basement to parts of the Palaeozoic St. Lawrence Lowlands in Quebec, Canada, suggests that this potential should be reassessed. However, such a task remains hard to achieve due to scarcity of heat flow data and ambiguity about the nature of the basement. To get an appraisal, the impact of radiogenic heat production for different Grenville Province crystalline basement units on temperature distribution at depth was simulated using the Underworld Geothermal numerical modelling code. The region south of Trois-Rivières was selected as representative for the St. Lawrence Lowlands. An existing 3D geological model based on well log data, seismic profiles and surface geology was used to build a catalogue of plausible thermal models. Statistical analyses of radiogenic element (U, Th, K) concentrations from neighbouring outcropping Grenville domains indicate that the radiogenic heat production of rocks in the modelled region is in the range of 0.34-3.24 μW/m3, with variations in the range of 0.94-5.83 μW/m3 for the Portneuf-Mauricie (PM) Domain, 0.02-4.13 μW/m3 for the Shawinigan Domain (Morin Terrane), and 0.34-1.96 μW/m3 for the Parc des Laurentides (PDL) Domain. Various scenarios considering basement characteristics similar to the PM domain, Morin Terrane and PDL Domain were modelled. The results show that the temperature difference between the scenarios can be as much as 12 °C at a depth of 5 km. The results also show that the temperature distribution is strongly affected by both the concentration of radiogenic elements and the thermal conductivity of the basement rocks. The thermal conductivity in the basement affects the trend of temperature change between two different geological units, and the spatial extent of thermal anomalies. The validity of the results was

  9. miRSponge: a manually curated database for experimentally supported miRNA sponges and ceRNAs.

    Science.gov (United States)

    Wang, Peng; Zhi, Hui; Zhang, Yunpeng; Liu, Yue; Zhang, Jizhou; Gao, Yue; Guo, Maoni; Ning, Shangwei; Li, Xia

    2015-01-01

    In this study, we describe miRSponge, a manually curated database, which aims at providing an experimentally supported resource for microRNA (miRNA) sponges. Recent evidence suggests that miRNAs are themselves regulated by competing endogenous RNAs (ceRNAs) or 'miRNA sponges' that contain miRNA binding sites. These competitive molecules can sequester miRNAs to prevent them interacting with their natural targets to play critical roles in various biological and pathological processes. It has become increasingly important to develop a high quality database to record and store ceRNA data to support future studies. To this end, we have established the experimentally supported miRSponge database that contains data on 599 miRNA-sponge interactions and 463 ceRNA relationships from 11 species following manual curating from nearly 1200 published articles. Database classes include endogenously generated molecules including coding genes, pseudogenes, long non-coding RNAs and circular RNAs, along with exogenously introduced molecules including viral RNAs and artificial engineered sponges. Approximately 70% of the interactions were identified experimentally in disease states. miRSponge provides a user-friendly interface for convenient browsing, retrieval and downloading of dataset. A submission page is also included to allow researchers to submit newly validated miRNA sponge data. Database URL: http://www.bio-bigdata.net/miRSponge. © The Author(s) 2015. Published by Oxford University Press.

  10. Mycobacterium tuberculosis decreases human macrophage IFN-γ responsiveness through miR-132 and miR-26a.

    Science.gov (United States)

    Ni, Bin; Rajaram, Murugesan V S; Lafuse, William P; Landes, Michelle B; Schlesinger, Larry S

    2014-11-01

    IFN-γ-activated macrophages play an essential role in controlling intracellular pathogens; however, macrophages also serve as the cellular home for the intracellular pathogen Mycobacterium tuberculosis. Based on previous evidence that M. tuberculosis can modulate host microRNA (miRNA) expression, we examined the miRNA expression profile of M. tuberculosis-infected primary human macrophages. We identified 31 differentially expressed miRNAs in primary human macrophages during M. tuberculosis infection by NanoString and confirmed our findings by quantitative real-time RT-PCR. In addition, we determined a role for two miRNAs upregulated upon M. tuberculosis infection, miR-132 and miR-26a, as negative regulators of transcriptional coactivator p300, a component of the IFN-γ signaling cascade. Knockdown expression of miR-132 and miR-26a increased p300 protein levels and improved transcriptional, translational, and functional responses to IFN-γ in human macrophages. Collectively, these data validate p300 as a target of miR-132 and miR-26a, and demonstrate a mechanism by which M. tuberculosis can limit macrophage responses to IFN-γ by altering host miRNA expression. Copyright © 2014 by The American Association of Immunologists, Inc.

  11. Early diagnostic evaluation of miR-122 and miR-224 as biomarkers for hepatocellular carcinoma

    Directory of Open Access Journals (Sweden)

    Khalda S. Amr

    2017-12-01

    Full Text Available Hepatocellular carcinoma (HCC is one of the common lethal types of tumor all over the world. The lethality of HCC accounts for many reasons. One of them, the lack of reliable diagnostic markers at the early stage, in this context, serum miRNAs became promising diagnostic biomarkers. Herein, we aimed to identify the predictive value of two miRNAs (miR-122 and miR-224 in plasma of patients with HCC preceded by chronic HCV infection. Taqman miRNA assays specific for hsa-miR-122 and hsa-miR-224 were used to assess the expression levels of the chosen miRNAs in plasma samples collected from three groups; 40 patients with HCC related to HCV, 40 with CHC patients and 20 healthy volunteers. This study revealed that the mean plasma values of miRNA-122 were significantly lower among HCC group when compared to CHC and control groups (P 1.2 (RQ and (AUC = 0.93, P < 0.001, while the accuracy of AFP to diagnose HCC was (AUC: 0.619; P = 0.06. In conclusion, the expression plasma of miR-122 and miR-224 could be used as noninvasive biomarkers for the early prediction of developing HCC at the early stage.

  12. MiRNA-155 and miRNA-132 as potential diagnostic biomarkers for pulmonary tuberculosis: A preliminary study.

    Science.gov (United States)

    Zheng, Meng-Li; Zhou, Nai-Kang; Luo, Cheng-Hua

    2016-11-01

    In our study, we aimed to profile a panel microRNAs (miRNAs) as potential biomarkers for the early diagnosis of pulmonary tuberculosis (PTB) and to illuminate the molecular mechanisms in the development of PTB. Firstly, gene expression profile of E-GEOD-49951 was downloaded from ArrayExpress database, and quantile-adjusted conditional maximum likelihood method was utilized to identify statistical difference between miRNAs of Mycobacterium tuberculosis (MTB)-infected individuals and healthy subjects. Furthermore, in order to assess the performance of our methodology, random forest (RF) classification model was utilized to identify the top 10 miRNAs with better Area Under The Curve (AUC) using 10-fold cross-validation method. Additionally, Monte Carlo Cross-Validation was repeated 50 times to explore the best miRNAs. In order to learn more about the differentially-expressed miRNAs, the target genes of differentially-expressed miRNAs were retrieved from TargetScan database and Ingenuity Pathways Analysis (IPA) was used to screen out biological pathways where target genes were involved. After normalization, a total of 478 miRNAs with higher than 0.25-fold quantile average across all samples were required. Based on the differential expression analysis, 38 differentially expressed miRNAs were identified when the significance was set as false discovery rate (FDR) < 0.01. Among the top 10 differentially expressed miRNAs, miRNA-155 obtained a highest AUC value 0.976, showing a good performance between PTB and control groups. Similarly, miRNA-449a, miRNA-212 and miRNA-132 revealed also a good performance with AUC values 0.947, 0.931 and 0.930, respectively. Moreover, miRNA-155, miRNA-449a, miRNA-29b-1* and miRNA-132 appeared in 50, 49, 49 and 48 bootstraps. Thus, miRNA-155 and miRNA-132 might be important in the progression of PTB and thereby, might present potential signatures for diagnosis of PTB. Copyright © 2016 Elsevier Ltd. All rights reserved.

  13. Superconducting wire for Lawrence Livermore National Laboratory in U.S.A

    International Nuclear Information System (INIS)

    Inoue, Itaru; Ikeda, Masaru; Tanaka, Yasuzo; Meguro, Shinichiro

    1985-01-01

    In Lawrence Livermore National Laboratory in USA, the development of a mirror type nuclear fusion reactor is carried out, and for plasma confinement, superconducting magnets are used. For the axicell coil generating a 12 T magnetic field in one of these magnets, Nb 3 Sn superconducting wires are to be used, and after the completion, it will be the largest magnet in the world as high magnetic field superconducting magnets. Furukawa Electric Co., Ltd. has completed the delivery of Nb 3 Sn superconducting wires used for this purpose. Since the Nb 3 Sn superconducting wires are very brittle, attention was paid to the manufacture to satisfy the required characteristics, and it was able to obtain the good reputation that the product was highly homogeneous as the superconducting wires of this type. In this paper, the design, manufacture and various characteristics of these superconducting wires are reported. The Nb 3 Sn superconducting wires were manufactured on industrial scale of 8 tons. The features of these Nb 3 Sn wires are the compound structure with semi-hard copper for low temperature stability and strengthening. (Kako, I.)

  14. The Secret Life of Archives: Sally Siddons, Sir Thomas Lawrence, and The Material of Memory

    Directory of Open Access Journals (Sweden)

    Laura Engel

    2014-03-01

    Full Text Available This essay is in two parts, in the first I attempt to map out strategies for considering archival materials through the lens of performance, and in the second I enact or perform some of those strategies through a close reading of a letter from Sally Siddons, daughter of the famous actress Sarah Siddons, to the renown portrait painter and rakish bad boy, Sir Thomas Lawrence. I present a methodology that considers archival researchers as tourists who approach archival objects and images as material for curating a virtual exhibition. I argue that this strategy allows us to recognize and attempt to envision the interdisciplinary relationship amongst archival materials in order to imagine them in spatial, theatrical, and visual proximity to one another. In this way as researchers we are performing a kind of re-enactment, an animation, of the secret life of archives, which attempts to account the embodied traces of the past by providing an accessible thought provoking map for audiences.

  15. Supplement analysis for paleontological excavation at the National Ignition Facility at Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    1997-01-01

    On December 15, 1997, contractor workers supporting the National Ignition Facility (NIF) construction uncovered bones suspected to be of paleontological importance. The NIF workers were excavating a utility trench near the southwest corner of the NIF footprint area, located at the northeast corner of the Lawrence Livermore National Laboratory (LLNL) Livermore Site, and were excavating at a depth of approximately 30 feet. Upon the discovery of bone fragments, the excavation in the immediate vicinity was halted and the LLNL archaeologist was notified. The archaeologist determined that there was no indication of cultural resources. Mark Goodwin, Senior Curator for the University of California Museum of Paleontology at the University of California, Berkeley, was then contacted. Mr. Goodwin visited the site on December 16th and confirmed that the bones consisted of a section of the skull, a portion of the mandible, several teeth, upper palate, and possibly the vertebrae of a mammoth, genus Mammuthus columbi. This supplement analysis evaluates the potential for adverse impacts of excavating skeletal remains, an activity that was only generally assessed by the NIF Project-Specific Analysis in the Final Programmatic Environmental impact Statement for Stockpile Stewardship and Management (SS and M PEIS) published in September 1996 (DOE/EIS-0236) and its Record of Decision published on December 19, 1996. This supplement analysis has been prepared pursuant to the DOE regulations implementing the National Environmental Policy Act (10 CFR 1021.314)

  16. Ernest Orlando Lawrence Berkeley National Laboratory institutional plan, FY 1996--2001

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-11-01

    The FY 1996--2001 Institutional Plan provides an overview of the Ernest Orlando Lawrence Berkeley National Laboratory mission, strategic plan, core business areas, critical success factors, and the resource requirements to fulfill its mission in support of national needs in fundamental science and technology, energy resources, and environmental quality. The Laboratory Strategic Plan section identifies long-range conditions that will influence the Laboratory, as well as potential research trends and management implications. The Core Business Areas section identifies those initiatives that are potential new research programs representing major long-term opportunities for the Laboratory, and the resources required for their implementation. It also summarizes current programs and potential changes in research program activity, science and technology partnerships, and university and science education. The Critical Success Factors section reviews human resources; work force diversity; environment, safety, and health programs; management practices; site and facility needs; and communications and trust. The Resource Projections are estimates of required budgetary authority for the Laboratory`s ongoing research programs. The Institutional Plan is a management report for integration with the Department of Energy`s strategic planning activities, developed through an annual planning process. The plan identifies technical and administrative directions in the context of the national energy policy and research needs and the Department of Energy`s program planning initiatives. Preparation of the plan is coordinated by the Office of Planning and Communications from information contributed by the Laboratory`s scientific and support divisions.

  17. Plasma experiments with 1.06-μm lasers at the Lawrence Livermore Laboratory

    International Nuclear Information System (INIS)

    Ahlstrom, H.G.; Holzrichter, J.F.; Manes, K.R.; Storm, E.K.; Boyle, M.J.; Brooks, K.M.; Haas, R.A.; Phillion, D.W.; Rupert, V.C.

    1976-01-01

    Recent laser fusion experiments at the Lawrence Livermore Laboratory have provided basic data concerning: laser beam propagation and absorption in high temperature plasmas, electron energy transport processes that transfer the absorbed laser energy to the high-density ablation region, the general fluid dynamic expansion and compression of the heated plasma, and the processes responsible for the production of 14-MeV neutrons during implosion experiments. Irradiation experiments were performed with Nd:YAG glass laser systems: the two-beam Janus (less than or equal to40 J/100 ps, approx.0.4 TW) and Argus (less than or equal to140 J, 35 ps, approx.4 TW), and the single beam Cyclops (less than or equal to70 J/100 ps, approx.0.7 TW). Two classes of targets have been used: glass microshells (approx.40 to 120 μm in diameter with approx.0.75-μm-thick walls) filled with an equimolar deuterium-tritium mixture, and disks (approx.160 to 600 μm in diameter and approx. 10 μm thick) of several compositions. The targets were supported in vacuum (pressure less than or equal to10 -5 Torr) by thin glass stalks. This paper reports on results related to the propagation, absorption, and scattering of laser light by both spherical and planar targets

  18. Estimating The Reliability of the Lawrence Livermore National Laboratory (LLNL) Flash X-ray (FXR) Machine

    International Nuclear Information System (INIS)

    Ong, M M; Kihara, R; Zentler, J M; Kreitzer, B R; DeHope, W J

    2007-01-01

    At Lawrence Livermore National Laboratory (LLNL), our flash X-ray accelerator (FXR) is used on multi-million dollar hydrodynamic experiments. Because of the importance of the radiographs, FXR must be ultra-reliable. Flash linear accelerators that can generate a 3 kA beam at 18 MeV are very complex. They have thousands, if not millions, of critical components that could prevent the machine from performing correctly. For the last five years, we have quantified and are tracking component failures. From this data, we have determined that the reliability of the high-voltage gas-switches that initiate the pulses, which drive the accelerator cells, dominates the statistics. The failure mode is a single-switch pre-fire that reduces the energy of the beam and degrades the X-ray spot-size. The unfortunate result is a lower resolution radiograph. FXR is a production machine that allows only a modest number of pulses for testing. Therefore, reliability switch testing that requires thousands of shots is performed on our test stand. Study of representative switches has produced pre-fire statistical information and probability distribution curves. This information is applied to FXR to develop test procedures and determine individual switch reliability using a minimal number of accelerator pulses

  19. 1-2 GeV synchrotron radiation facility at Lawrence Berkeley Laboratory

    International Nuclear Information System (INIS)

    Berkner, K.H.

    1985-10-01

    The Advanced Light Source (ALS), a dedicated synchrotron radiation facility optimized to generate soft x-ray and vacuum ultraviole (XUV) light using magnetic insertion devices, was proposed by the Lawrence Berkeley Laboratory in 1982. It consists of a 1.3-GeV injection system, an electron storage ring optimized at 1.3 GeV (with the capability of 1.9-GeV operation), and a number of photon beamlines emanating from twelve 6-meter-long straight sections, as shown in Fig. 1. In addition, 24 bending-magnet ports will be avialable for development. The ALS was conceived as a research tool whose range and power would stimulate fundamentally new research in fields from biology to materials science (1-4). The conceptual design and associated cost estimate for the ALS have been completed and reviewed by the US Department of Energy (DOE), but preliminary design activities have not yet begun. The focus in this paper is on the history of the ALS as an example of how a technical construction project was conceived, designed, proposed, and validated within the framwork of a national laboratory funded largely by the DOE

  20. Cost-benefit analysis for waste segregation at Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    1992-02-01

    This report presents a cost-benefit analysis for the segregation of mixed, hazardous, and nonhazardous wastes at Lawrence Livermore National Laboratory (LLNL). The cost-benefit analysis was conducted to determine if current waste segregation practices and additional candidates for waste segregation at LLNL might have the potential for significant waste source reduction and annual savings in treatment and disposal costs. In the following cost-benefit analysis, capital costs and recurring costs of waste segregation practices are compared to the economic benefits of savings in treatment and disposal costs. Indirect or overhead costs associated with these wastes are not available and have not been included. Not considered are additional benefits of waste segregation such as decreased potential for liability to LLNL for adverse environmental effects, improved worker safety, and enhanced LLNL image within the community because of environmental improvement. The economic evaluations in this report are presented on a Lab-wide basis. All hazardous wastes generated by a program are turned over to the Hazardous Waste Management (HWM) group, which is responsible for the storage, treatment, or disposal of these wastes and funded funded directly for this work

  1. Summary Report of Summer 2009 NGSI Human Capital Development Efforts at Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Dougan, A; Dreicer, M; Essner, J; Gaffney, A; Reed, J; Williams, R

    2009-11-16

    In 2009, Lawrence Livermore National Laboratory (LLNL) engaged in several activities to support NA-24's Next Generation Safeguards Initiative (NGSI). This report outlines LLNL's efforts to support Human Capital Development (HCD), one of five key components of NGSI managed by Dunbar Lockwood in the Office of International Regimes and Agreements (NA-243). There were five main LLNL summer safeguards HCD efforts sponsored by NGSI: (1) A joint Monterey Institute of International Studies/Center for Nonproliferation Studies-LLNL International Safeguards Policy and Information Analysis Course; (2) A Summer Safeguards Policy Internship Program at LLNL; (3) A Training in Environmental Sample Analysis for IAEA Safeguards Internship; (4) Safeguards Technology Internships; and (5) A joint LLNL-INL Summer Safeguards Lecture Series. In this report, we provide an overview of these five initiatives, an analysis of lessons learned, an update on the NGSI FY09 post-doc, and an update on students who participated in previous NGSI-sponsored LLNL safeguards HCD efforts.

  2. Building an internet-based workflow system - the case of Lawrence Livermore National Laboratories` Zephyr project

    Energy Technology Data Exchange (ETDEWEB)

    Jordan, C. W., LLNL

    1998-04-01

    Lawrence Livermore National Laboratories` Zephyr System provides a showcase for the ways in which emerging technologies can help streamline procurement processes and improve the coordination between participants in engineering projects by allowing collaboration in ways that have not been possible before. The project also shows the success of a highly pragmatic approach that was initiated by the end user community, and that intentionally covered standard situations, rather than aiming at also automating the exceptions. By helping push purchasing responsibilities down to the end user, thereby greatly reducing the involvement of the purchasing department in operational activities, it was possible to streamline the process significantly resulting in time savings of up to 90%, major cost reductions, and improved quality. Left with less day-to- day purchasing operations, the purchasing department has more time for strategic tasks such as selecting and pre-qualifying new suppliers, negotiating blanket orders, or implementing new procurement systems. The case shows once more that the use of information technologies can result in major benefits when aligned with organizational adjustments.

  3. Construction quality assurance for Pit 6 landfill closure, Lawrence Livermore National Laboratory, Site 300

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-10-30

    Golder Construction Services, Inc. (GCS), under contract to the Regents of the University of California, Lawrence Livermore National Laboratory (LLNL), provided the construction quality assurance (CQA) observation and testing during the construction of the Site 300, Pit 6 landfill closure cover. The cap construction was performed as a CERCLA non-time-critical removal action from June 2 to August 29, 1997. the project site is located 18 miles east of Livermore on Tesla Road and approximately 10 miles southwest of Tracy on Corral Hollow Road in San Joaquin County, California. This report certifies that the LLNL, Site 300, Pit 6, Landfill Closure was constructed in accordance with the construction specifications and design drawings. This report documents construction activities and CQA monitoring and testing for construction of the Pit 6 Landfill Closure. Golder Associates, Inc. of Oakland, California was the design engineering firm responsible for preparation of the drawings and specifications. CQA services were provided by GCS, of Roseville, California, under supervision of a California registered civil Engineer.

  4. Current and future health physics research at Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    Hickman, D.P.

    1993-01-01

    Lawrence Livermore National Laboratory has developed several radiation protection instruments and continues to pursue new approaches in this area. Some of the instruments developed include innovative air-monitoring systems; neutron detection and dosimetry systems; specialized calibration materials and structures, such as the LLNL Realistic Torso Phantom; a fast-response detector system to detect stray beams from x-ray fluorescence devices that can be manufactured for less than $600; and a reliable, light weight personnel air-monitoring system that can be incorporated into a security badge/dosimeter package. A multi-disciplinary team of experts at LLNL is developing and testing cleanable/reusable high-efficiency particulate air-filtration systems and highly sensitive instrumentation for differentiating transuranic waste from nontransuranic waste; developing an advanced detector and circuit design for a hand-held neutron spectrometer; developing techniques for detecting neutron sources using CR-39 and for calibrating in-vivo measurement equipment using Magnetic Resonance Imaging and Monte Carlo simulation; and developing a seamless bottle mannequin adsorption (BOMAB) phantom with recessed fill caps, which have no potential for leakage of liquid sources used for calibrating whole-body counters

  5. Quebec region's shoreline segmentation in the St. Lawrence River : response tool for oil spills

    International Nuclear Information System (INIS)

    Laforest, S.; Martin, V.

    2004-01-01

    Environment Canada, the Canadian Coast Guard, and the Eastern Canada Response Corporation are developing and refining pre-spill databases containing information about physical shoreline characteristics. Automated links between these pre-spill shoreline characteristic databases and computerized shoreline assessment tools have also been created using Geographical Information System (GIS) technology. The pre-spill databases can be used for planning shoreline cleanup operations. A training exercise, designed to evaluate a spill management system integrating the Quebec region pre-spill shoreline database and the ShoreAssess R shoreline assessment system was performed by Eastern Canada Response Corporation during an aerial survey where shoreline was segmented into digitized information. The cartography of segmentation covers the fluvial part of the St. Lawrence River. The oil spill-oriented database includes geomorphologic information from the supratidal to the lower intertidal zones. It also includes some statistical information and other requirements for cleanup operations. The computerized shoreline assessment tools made it possible to evaluate the length and type of shoreline that would potentially be impacted by oil. The tools also made it possible to assess the shoreline treatment methods most likely to be used, and evaluate the probable duration of the cleanup operation. The training exercise demonstrated that the integration of the databases is a valuable tool during the early phases of an oil spill response. 9 refs., 3 figs

  6. Final Safety Analysis Document for Building 693 Chemical Waste Storage Building at Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    Salazar, R.J.; Lane, S.

    1992-02-01

    This Safety Analysis Document (SAD) for the Lawrence Livermore National Laboratory (LLNL) Building 693, Chemical Waste Storage Building (desipated as Building 693 Container Storage Unit in the Laboratory's RCRA Part B permit application), provides the necessary information and analyses to conclude that Building 693 can be operated at low risk without unduly endangering the safety of the building operating personnel or adversely affecting the public or the environment. This Building 693 SAD consists of eight sections and supporting appendices. Section 1 presents a summary of the facility designs and operations and Section 2 summarizes the safety analysis method and results. Section 3 describes the site, the facility desip, operations and management structure. Sections 4 and 5 present the safety analysis and operational safety requirements (OSRs). Section 6 reviews Hazardous Waste Management's (HWM) Quality Assurance (QA) program. Section 7 lists the references and background material used in the preparation of this report Section 8 lists acronyms, abbreviations and symbols. Appendices contain supporting analyses, definitions, and descriptions that are referenced in the body of this report

  7. Environmental assessment for the electric utility system distribution, replacements and upgrades at Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    1992-04-01

    This Environmental Assessment evaluates the environmental effects resulting from the distribution of new electrical service, replacement of inadequate or aging equipment, and upgrade of the existing electrical utility system at Lawrence Livermore National Laboratory. The projects assessed herein do not impact cultural or historic resources, sensitive habitats or wetlands and are not a source of air emissions. The potential environmental effects that do result from the action are fugitive dust and noise from construction and the disposal of potentially contaminated soil removed from certain limited areas of the LLNL site as a result of trenching for underground transmission lines. The actions described in this assessment represent an improved safety and reliability to the existing utility system. Inherent in the increased reliability and upgrades is a net increase in electrical capacity, with future expansion reserve. As with any electrical device, the electrical utility system has associated electric and magnetic fields that present a potential source of personnel exposure. The potential is not increased, however, beyond that which already exists for the present electrical utility system

  8. On-line monitoring of toxic materials in sewage at the Lawrence Livermore Laboratory

    International Nuclear Information System (INIS)

    Auyong, M.; Cate, J.L. Jr.; Rueppel, D.W.

    1980-01-01

    It is becoming increasingly important for industry to prevent releases of potentially toxic material to the environment. The Lawrence Livermore Laboratory has developed a system to monitor its sewage effluent on a continuous basis. A representative fraction of the total waste stream leaving the Plant is passed through a detection assembly consisting of an x-ray fluorescence unit which detects high levels of metals, sodium iodide crystal detectors that scan the sewage for the presence of elevated levels of radiation, and an industrial probe for pH monitoring. With the aid of a microprocessor, the data collected is reduced and analyzed to determine whether levels are approaching established environmental limits. Currently, if preset pH or radiation levels are exceeded, a sample of the suspect sewage is automatically collected for further analysis, and an alarm is sent to a station where personnel can be alerted to respond on a 24-hour basis. In the same manner, spectral data from the x-ray fluorescence unit will be routed through the 24-hour alarm system as soon as evaluation of the unit is complete. The design of the system and operational experience is discussed

  9. Fire History of Appalachian Forests of the Lower St-Lawrence Region (Southern Quebec

    Directory of Open Access Journals (Sweden)

    Serge Payette

    2017-04-01

    Full Text Available Sugar maple (Acer saccharum forests are among the main forest types of eastern North America. Sugar maple stands growing on Appalachian soils of the Lower St-Lawrence region are located at the northeastern limit of the northern hardwood forest zone. Given the biogeographical position of these forests at the edge of the boreal biome, we aimed to reconstruct the fire history and document the occurrence of temperate and boreal trees in sugar maple sites during the Holocene based on soil macrocharcoal analysis. Despite having experienced a different number of fire events, the fire history of the maple sites was broadly similar, with two main periods of fire activity, i.e., early- to mid-Holocene and late-Holocene. A long fire-free interval of at least 3500 years separated the two periods from the mid-Holocene to 2000 years ago. The maple sites differ with respect to fire frequency and synchronicity of the last millennia. According to the botanical composition of charcoal, forest vegetation remained relatively homogenous during the Holocene, except recently. Conifer and broadleaf species coexisted in mixed forests during the Holocene, in phase with fire events promoting the regeneration of boreal and temperate tree assemblages including balsam fir (Abies balsamea and sugar maple.

  10. The Advanced Light Source at Lawrence Berkeley Laboratory: a new tool for research in atomic physics

    International Nuclear Information System (INIS)

    Schlachter, A.S.; Robinson, A.L.

    1991-01-01

    The Advanced Light Source, a third-generation national synchrotron-radiation facility now under construction at the Lawrence Berkeley Laboratory, is scheduled to begin serving qualified users across a broad spectrum of research areas in the spring of 1993. Based on a low-emittance electron storage ring optimized to operate at 1.5 GeV, the ALS will have 10 long straight sections available for insertion devices (undulators and wigglers) and 24 high-quality bend-magnet ports. The short pulse width (30-50 ps) will be ideal for time-resolved measurements. Undulators will generate high-brightness partially coherent soft X-ray and ultraviolet (XUV) radiation from below 10 eV to above 2 keV; this radiation is plane polarized. Wigglers and bend magnets will extend the spectrum by generating high fluxes of X-rays to photon energies above 10 keV. The ALS will have an extensive research program in which XUV radiation is used to study matter in allk its varied gaseous, liquid, and solid forms. The high brightness will open new areas of research in the materials sciences, such as spatially resolved spectroscopy (spectromicroscopy), and in biology, such as X-ray microscopy with element-specific sensitivity; the high flux will allow measurements in atomic physics and chemistry to be made with tenuous gas-phase targets. Technological applications could include lithography and nano-fabrication. (orig.)

  11. Lawrence Livermore National Laboratory underground coal gasification data base. [US DOE-supported field tests; data

    Energy Technology Data Exchange (ETDEWEB)

    Cena, R. J.; Thorsness, C. B.

    1981-08-21

    The Department of Energy has sponsored a number of field projects to determine the feasibility of converting the nation's vast coal reserves into a clean efficient energy source via underground coal gasification (UCG). Due to these tests, a significant data base of process information has developed covering a range of coal seams (flat subbituminous, deep flat bituminous and steeply dipping subbituminous) and processing techniques. A summary of all DOE-sponsored tests to data is shown. The development of UCG on a commercial scale requires involvement from both the public and private sectors. However, without detailed process information, accurate assessments of the commercial viability of UCG cannot be determined. To help overcome this problem the DOE has directed the Lawrence Livermore National Laboratory (LLNL) to develop a UCG data base containing raw and reduced process data from all DOE-sponsored field tests. It is our intent to make the data base available upon request to interested parties, to help them assess the true potential of UCG.

  12. A Comparison between Local and Global Spaceborne Chlorophyll Indices in the St. Lawrence Estuary

    Directory of Open Access Journals (Sweden)

    Martin A. Montes-Hugo

    2012-11-01

    Full Text Available Spaceborne chlorophyll indices based on red fluorescence (wavelength = 680 nm and water leaving radiance (Lw in the visible spectrum (i.e., 400–700 nm were evaluated in the St Lawrence Estuary (SLE during September of 2011. Relationships between chlorophyll concentration (chl and fluorescence were constructed based on fluorescence line height (FLH measurements derived from a compact laser-based spectrofluorometer developed by ENEA (CASPER and using spectral bands corresponding to the satellite sensor MERIS (MEdium Resolution Imaging Spectrometer. Chlorophyll concentration as estimated from CASPER (chlCASPER was relatively high NE of the MTZ (upper Estuary, and nearby areas influenced by fronts or freshwater plumes derived from secondary rivers (lower estuary. These findings agree with historical shipboard measurements. In general, global chl products calculated from Lw had large biases (up to 27-fold overestimation and 50-fold underestimation with respect to chlCASPER values. This was attributed to the smaller interference of detritus (mineral + organic non-living particulates and chromophoric dissolved organic matter on chlCASPER estimates. We encourage the use of spectrofluorometry for developing and validating remote sensing models of chl in SLE waters and other coastal environments characterized by relatively low to moderate (<10 g·m−3 concentrations of detritus.

  13. Workplace investigation of increased diagnosis of malignant melanoma among employees of Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Moore, D.H. II; Patterson, H.W.; Hatch, F.; Discher, D.; Schneider, J.S.; Bennett, D.

    1994-08-01

    Based on rates for the surrounding communities, the diagnosis rate of malignant melanoma for employees of Lawrence Livermore National Laboratory (LLNL) during 1972 to 1977 was three to four times higher than expected. In 1984 Austin and Reynolds concluded, as a result of a case-control study, that five occupational factors were {open_quotes}causally associated{close_quotes} with melanoma risk at LLNL. These factors were: (1) exposure to radioactive materials, (2) work at Site 300, (3) exposure to volatile photographic chemicals, (4) presence at the Pacific Test Site, and (5) chemist duties. Subsequent reviews of the Austin and Reynolds report concluded that the methods used were appropriate and correctly carried out. These reports did determine, however, that Austin and Reynolds` conclusion concerning a causal relationship between occupational factors and melanoma among employees was overstated. There is essentially no supporting evidence linking the occupational factors with melanoma from animal studies or human epidemiology. Our report summarizes the results of further investigation of potential occupational factors.

  14. Site safety plan for Lawrence Livermore National Laboratory CERCLA investigations at site 300. Revision 2

    Energy Technology Data Exchange (ETDEWEB)

    Kilmer, J.

    1997-08-01

    Various Department of Energy Orders incorporate by reference, health and safety regulations promulgated by the Occupational Safety and Health Administration (OSHA). One of the OSHA regulations, 29 CFR 1910.120, Hazardous Waste Operations and Emergency Response, requires that site safety plans are written for activities such as those covered by work plans for Site 300 environmental investigations. Based upon available data, this Site Safety Plan (Plan) for environmental restoration has been prepared specifically for the Lawrence Livermore National Laboratory Site 300, located approximately 15 miles east of Livermore, California. As additional facts, monitoring data, or analytical data on hazards are provided, this Plan may need to be modified. It is the responsibility of the Environmental Restoration Program and Division (ERD) Site Safety Officer (SSO), with the assistance of Hazards Control, to evaluate data which may impact health and safety during these activities and to modify the Plan as appropriate. This Plan is not `cast-in-concrete.` The SSO shall have the authority, with the concurrence of Hazards Control, to institute any change to maintain health and safety protection for workers at Site 300.

  15. Public census data on CD-ROM at Lawrence Berkeley Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Merrill, D.W.

    1992-10-01

    The Comprehensive Epidemiologic Data Resource (CEDR) and Populations at Risk to Environmental Pollution (PAREP) projects, of the Information and Computing Sciences Division (ICSD) at Lawrence Berkeley Laboratory (LBL), are using public socio-economic and geographic data files which are available to CEDR and PAREP collaborators via LBL's computing network. At this time 70 CD-ROM diskettes (approximately 36 gigabytes) are on line via the Unix file server cedrcd. lbl. gov. Most of the files are from the US Bureau of the Census, and most pertain to the 1990 Census of Population and Housing. All the CD-ROM diskettes contain documentation in the form of ASCII text files. Printed documentation for most files is available for inspection at University of California Data and Technical Assistance (UC DATA), or the UC Documents Library. Many of the CD-ROM diskettes distributed by the Census Bureau contain software for PC compatible computers, for easily accessing the data. Shared access to the data is maintained through a collaboration among the CEDR and PAREP projects at LBL, and UC DATA, and the UC Documents Library. Via the Sun Network File System (NFS), these data can be exported to Internet computers for direct access by the user's application program(s).

  16. Public census data on CD-ROM at Lawrence Berkeley Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Merrill, D.W.

    1992-10-01

    The Comprehensive Epidemiologic Data Resource (CEDR) and Populations at Risk to Environmental Pollution (PAREP) projects, of the Information and Computing Sciences Division (ICSD) at Lawrence Berkeley Laboratory (LBL), are using public socio-economic and geographic data files which are available to CEDR and PAREP collaborators via LBL`s computing network. At this time 70 CD-ROM diskettes (approximately 36 gigabytes) are on line via the Unix file server cedrcd. lbl. gov. Most of the files are from the US Bureau of the Census, and most pertain to the 1990 Census of Population and Housing. All the CD-ROM diskettes contain documentation in the form of ASCII text files. Printed documentation for most files is available for inspection at University of California Data and Technical Assistance (UC DATA), or the UC Documents Library. Many of the CD-ROM diskettes distributed by the Census Bureau contain software for PC compatible computers, for easily accessing the data. Shared access to the data is maintained through a collaboration among the CEDR and PAREP projects at LBL, and UC DATA, and the UC Documents Library. Via the Sun Network File System (NFS), these data can be exported to Internet computers for direct access by the user`s application program(s).

  17. Assessment and cleanup of the Taxi Strip waste storage area at LLNL [Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    Buerer, A.

    1983-01-01

    In September 1982 the Hazards Control Department of the Lawrence Livermore National Laboratory (LLNL) began a final radiological survey of a former low-level radioactive waste storage area called the Taxi Strip so that the area could be released for construction of an office building. Collection of soil samples at the location of a proposed sewer line led to the discovery of an old disposal pit containing soil contaminated with low-level radioactive waste and organic solvents. The Taxi Strip area was excavated leading to the discovery of three additional small pits. The clean-up of Pit No. 1 is considered to be complete for radioactive contamination. The results from the chlorinated solvent analysis of the borehole samples and the limited number of samples analyzed by gas chromatography/mass spectrometry indicate that solvent clean-up at this pit is complete. This is being verified by gas chromatography/mass spectrometry analysis of a few additional soil samples from the bottom sides and ends of the pit. As a precaution, samples are also being analyzed for metals to determine if further excavation is necessary. Clean-up of Pits No. 2 and No. 3 is considered to be complete for radioactive and solvent contamination. Results of analysis for metals will determine if excavation is complete. Excavation of Pit No. 4 which resulted from surface leakage of radioactive contamination from an evaporation tray is complete

  18. Update of Earthquake Strong-Motion Instrumentation at Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Murray, Robert C. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2013-09-01

    Following the January 1980 earthquake that was felt at Lawrence Livermore National Laboratory (LLNL), a network of strong-motion accelerographs was installed at LLNL. Prior to the 1980 earthquake, there were no accelerographs installed. The ground motion from the 1980 earthquake was estimated from USGS instruments around the Laboratory to be between 0.2 – 0.3 g horizontal peak ground acceleration. These instruments were located at the Veterans Hospital, 5 miles southwest of LLNL, and in San Ramon, about 12 miles west of LLNL. In 2011, the Department of Energy (DOE) requested to know the status of our seismic instruments. We conducted a survey of our instrumentation systems and responded to DOE in a letter. During this survey, it was found that the recorders in Buildings 111 and 332 were not operational. The instruments on Nova had been removed, and only three of the 10 NIF instruments installed in 2005 were operational (two were damaged and five had been removed from operation at the request of the program). After the survey, it was clear that the site seismic instrumentation had degraded substantially and would benefit from an overhaul and more attention to ongoing maintenance. LLNL management decided to update the LLNL seismic instrumentation system. The updated system is documented in this report.

  19. Summary Report of Summer 2009 NGSI Human Capital Development Efforts at Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    Dougan, A.; Dreicer, M.; Essner, J.; Gaffney, A.; Reed, J.; Williams, R.

    2009-01-01

    In 2009, Lawrence Livermore National Laboratory (LLNL) engaged in several activities to support NA-24's Next Generation Safeguards Initiative (NGSI). This report outlines LLNL's efforts to support Human Capital Development (HCD), one of five key components of NGSI managed by Dunbar Lockwood in the Office of International Regimes and Agreements (NA-243). There were five main LLNL summer safeguards HCD efforts sponsored by NGSI: (1) A joint Monterey Institute of International Studies/Center for Nonproliferation Studies-LLNL International Safeguards Policy and Information Analysis Course; (2) A Summer Safeguards Policy Internship Program at LLNL; (3) A Training in Environmental Sample Analysis for IAEA Safeguards Internship; (4) Safeguards Technology Internships; and (5) A joint LLNL-INL Summer Safeguards Lecture Series. In this report, we provide an overview of these five initiatives, an analysis of lessons learned, an update on the NGSI FY09 post-doc, and an update on students who participated in previous NGSI-sponsored LLNL safeguards HCD efforts.

  20. Mixed waste study, Lawrence Livermore National Laboratory Hazardous Waste Management facilities

    International Nuclear Information System (INIS)

    1990-11-01

    This document addresses the generation and storage of mixed waste at Lawrence Livermore National Laboratory (LLNL) from 1984 to 1990. Additionally, an estimate of remaining storage capacity based on the current inventory of low-level mixed waste and an approximation of current generation rates is provided. Section 2 of this study presents a narrative description of Environmental Protection Agency (EPA) and Department of Energy (DOE) requirements as they apply to mixed waste in storage at LLNL's Hazardous Waste Management (HWM) facilities. Based on information collected from the HWM non-TRU radioactive waste database, Section 3 presents a data consolidation -- by year of storage, location, LLNL generator, EPA code, and DHS code -- of the quantities of low-level mixed waste in storage. Related figures provide the distribution of mixed waste according to each of these variables. A historical review follows in Section 4. The trends in type and quantity of mixed waste managed by HWM during the past five years are delineated and graphically illustrated. Section 5 provides an estimate of remaining low-level mixed waste storage capacity at HWM. The estimate of remaining mixed waste storage capacity is based on operational storage capacity of HWM facilities and the volume of all waste currently in storage. An estimate of the time remaining to reach maximum storage capacity is based on waste generation rates inferred from the HWM database and recent HWM documents. 14 refs., 18 figs., 9 tabs

  1. Vadose zone investigations at the Lawrence Livermore National Laboratory Superfund Site: An overview

    International Nuclear Information System (INIS)

    Iovenitti, J.L.; Nitao, J.J.; Bishop, D.J.

    1992-09-01

    Lawrence Livermore National Laboratory (LLNL)is investigating the fate and transport of vadose zone contaminants at their Livermore site in Livermore, California. The principal objectives of this work are to identify potential source areas at the Livermore site which require remediation, to prioritize those areas, and finally, to optimize the remediation process. Primary contaminants of interest for this investigation are volatile organic compounds (VOCs) and tritium. A fully integrated, three-part program, consisting of quantitative modeling, field studies, and laboratory measurements, is in progress. To evaluate and predict vadose zone contaminant migration, quantitative modeling is used. Our modeling capabilities are being enhanced through the development of a multicomponent,three-dimensional,nonaqueous phase liquid-liquid-vapor,nonisothermal flow and transport computer code. This code will be also used to evaluate vadose zone remediation requirements. Field studies to acquire LLNL site-specific soil (sediment) characteristics for computer code calibration and validation include subsurf ace lithologic and contaminant profiling, in situ soil moisture content, ground surface emission flux of VOCs and tritium, transpiration of tritium, and ground surface evapotranspiration of water. Multilevel vadose zone monitoring devices are used to monitor the gaseous and aqueous transport of contaminants

  2. Ernest Orlando Lawrence Berkeley National Laboratory Institutional Plan FY 2000-2004

    Energy Technology Data Exchange (ETDEWEB)

    Chartock, Mike (ed.); Hansen, Todd (ed.)

    1999-08-01

    The FY 2000-2004 Institutional Plan provides an overview of the Ernest Orlando Lawrence Berkeley National Laboratory (Berkeley Lab, the Laboratory) mission, strategic plan, initiatives, and the resources required to fulfill its role in support of national needs in fundamental science and technology, energy resources, and environmental quality. To advance the Department of Energy's ongoing efforts to define the Integrated Laboratory System, the Berkeley Lab Institutional Plan reflects the strategic elements of our planning efforts. The Institutional Plan is a management report that supports the Department of Energy's mission and programs and is an element of the Department of Energy's strategic management planning activities, developed through an annual planning process. The Plan supports the Government Performance and Results Act of 1993 and complements the performance-based contract between the Department of Energy and the Regents of the University of California. It identifies technical and administrative directions in the context of the national energy policy and research needs and the Department of Energy's program planning initiatives. Preparation of the plan is coordinated by the Office of Planning and Communications from information contributed by Berkeley Lab's scientific and support divisions.

  3. Incubation of human blood fractions leads to changes in apparent miRNA abundance

    DEFF Research Database (Denmark)

    Skovgaard, Kerstin; Jørgensen, Stine Thuen; Heegaard, Peter M. H.

    in significant changes in the abundance of miR-21, miR-155, Let-7c and Let 7f in plasma, miR-21, miR-23a and miR-150 in RBC and miR-15b, miR-126, miR155 and Let-7g in PBMC, while no change was seen in PRP and PMN. Interestingly, in the samples incubated with glass beads, no miRNAs were significantly affected...... in plasma, RBC, PBMC and PMN, while expression of miR-25, miR15a, miR-126 and miR223 was significantly changed in PRP. Thus, PRP, as the only blood fraction depended on stimulation to change its miRNA profile upon incubation. For the other fractions, stimulation either leveled out the changes induced...

  4. Characterization of miRNomes in Acute and Chronic Myeloid

    Directory of Open Access Journals (Sweden)

    Qian Xiong

    2014-04-01

    Full Text Available Myeloid leukemias are highly diverse diseases and have been shown to be associated with microRNA (miRNA expression aberrations. The present study involved an in-depth miRNome analysis of two human acute myeloid leukemia (AML cell lines, HL-60 and THP-1, and one human chronic myeloid leukemia (CML cell line, K562, via massively parallel signature sequencing. mRNA expression profiles of these cell lines that were established previously in our lab facilitated an integrative analysis of miRNA and mRNA expression patterns. miRNA expression profiling followed by differential expression analysis and target prediction suggested numerous miRNA signatures in AML and CML cell lines. Some miRNAs may act as either tumor suppressors or oncomiRs in AML and CML by targeting key genes in AML and CML pathways. Expression patterns of cell type-specific miRNAs could partially reflect the characteristics of K562, HL-60 and THP-1 cell lines, such as actin filament-based processes, responsiveness to stimulus and phagocytic activity. miRNAs may also regulate myeloid differentiation, since they usually suppress differentiation regulators. Our study provides a resource to further investigate the employment of miRNAs in human leukemia subtyping, leukemogenesis and myeloid development. In addition, the distinctive miRNA signatures may be potential candidates for the clinical diagnosis, prognosis and treatment of myeloid leukemias.

  5. Expression and evolutionary analyses of three acetylcholinesterase genes (Mi-ace-1, Mi-ace-2, Mi-ace-3) in the root-knot nematode Meloidogyne incognita.

    Science.gov (United States)

    Cui, Ruqiang; Zhang, Lei; Chen, Yuyan; Huang, Wenkun; Fan, Chengming; Wu, Qingsong; Peng, Deliang; da Silva, Washington; Sun, Xiaotang

    2017-05-01

    The full cDNA of Mi-ace-3 encoding an acetylcholinesterase (AChE) in Meloidogyne incognita was cloned and characterized. Mi-ace-3 had an open reading frame of 1875 bp encoding 624 amino acid residues. Key residues essential to AChE structure and function were conserved. The deduced Mi-ACE-3 protein sequence had 72% amino acid similarity with that of Ditylenchus destructor Dd-AChE-3. Phylogenetic analyses using 41 AChEs from 24 species showed that Mi-ACE-3 formed a cluster with 4 other nematode AChEs. Our results revealed that the Mi-ace-3 cloned in this study, which is orthologous to Caenorhabditis elegans AChE, belongs to the nematode ACE-3/4 subgroup. There was a significant reduction in the number of galls in transgenic tobacco roots when Mi-ace-1, Mi-ace-2, and Mi-ace-3 were knocked down simultaneously, whereas little or no effect were observed when only one or two of these genes were knocked down. This is an indication that the functions of these three genes are redundant. Copyright © 2017. Published by Elsevier Inc.

  6. Novel Triazole linked 2-phenyl benzoxazole derivatives induce apoptosis by inhibiting miR-2, miR-13 and miR-14 function in Drosophila melanogaster.

    Science.gov (United States)

    Mondal, Tanmoy; Lavanya, A V S; Mallick, Akash; Dadmala, Tulshiram L; Kumbhare, Ravindra M; Bhadra, Utpal; Bhadra, Manika Pal

    2017-06-01

    Apoptosis is an important phenomenon in multi cellular organisms for maintaining tissue homeostasis and embryonic development. Defect in apoptosis leads to a number of disorders like- autoimmune disorder, immunodeficiency and cancer. 21-22 nucleotides containing micro RNAs (miRNAs/miRs) function as a crucial regulator of apoptosis alike other cellular pathways. Recently, small molecules have been identified as a potent inducer of apoptosis. In this study, we have identified novel Triazole linked 2-phenyl benzoxazole derivatives (13j and 13h) as a negative regulator of apoptosis inhibiting micro RNAs (miR-2, miR-13 and miR-14) in a well established in vivo model Drosophila melanogaster where the process of apoptosis is very similar to human apoptosis. These compounds inhibit miR-2, miR-13 and miR-14 activity at their target sites, which induce an increased caspase activity, and in turn influence the caspase dependent apoptotic pathway. These two compounds also increase the mitochondrial reactive oxygen species (ROS) level to trigger apoptotic cell death.

  7. miR-181a and miR-630 regulate cisplatin-induced cancer cell death.

    Science.gov (United States)

    Galluzzi, Lorenzo; Morselli, Eugenia; Vitale, Ilio; Kepp, Oliver; Senovilla, Laura; Criollo, Alfredo; Servant, Nicolas; Paccard, Caroline; Hupé, Philippe; Robert, Thomas; Ripoche, Hugues; Lazar, Vladimir; Harel-Bellan, Annick; Dessen, Philippe; Barillot, Emmanuel; Kroemer, Guido

    2010-03-01

    MicroRNAs (miRNA) are noncoding RNAs that regulate multiple cellular processes, including proliferation and apoptosis. We used microarray technology to identify miRNAs that were upregulated by non-small cell lung cancer (NSCLC) A549 cells in response to cisplatin (CDDP). The corresponding synthetic miRNA precursors (pre-miRNAs) per se were not lethal when transfected into A549 cells yet affected cell death induction by CDDP, C2-ceramide, cadmium, etoposide, and mitoxantrone in an inducer-specific fashion. Whereas synthetic miRNA inhibitors (anti-miRNAs) targeting miR-181a and miR-630 failed to modulate the response of A549 to CDDP, pre-miR-181a and pre-miR-630 enhanced and reduced CDDP-triggered cell death, respectively. Pre-miR-181a and pre-miR-630 consistently modulated mitochondrial/postmitochondrial steps of the intrinsic pathway of apoptosis, including Bax oligomerization, mitochondrial transmembrane potential dissipation, and the proteolytic maturation of caspase-9 and caspase-3. In addition, pre-miR-630 blocked early manifestations of the DNA damage response, including the phosphorylation of the ataxia-telangiectasia mutated (ATM) kinase and of two ATM substrates, histone H2AX and p53. Pharmacologic and genetic inhibition of p53 corroborated the hypothesis that pre-miR-630 (but not pre-miR-181a) blocks the upstream signaling pathways that are ignited by DNA damage and converge on p53 activation. Pre-miR-630 arrested A549 cells in the G0-G1 phase of the cell cycle, correlating with increased levels of the cell cycle inhibitor p27(Kip1) as well as with reduced proliferation rates and resulting in greatly diminished sensitivity of A549 cells to the late S-G2-M cell cycle arrest mediated by CDDP. Altogether, these results identify miR-181a and miR-630 as novel modulators of the CDDP response in NSCLC.

  8. Assessing potential impacts of climate change and variability on the Great Lakes-St. Lawrence Basin: A binational approach

    International Nuclear Information System (INIS)

    Quinn, F.H.; Mortsch, L.D.

    1997-01-01

    The potential impacts of climate change and variability on the Great Lakes environment are serious and complex. The Great Lakes-St. Lawrence Basin is home to 42.5 million US and Canadian citizens and is the industrial and commercial heartland of both nations. The region is rich in human and natural resources, with diverse economic activities and substantial infrastructure which would be affected by major shifts in climate. For example, water level changes could affect wetland distribution and functioning; reductions in streamflow would alter assimilative capacities while warmer water temperatures would influence spring and fall turnover and incidence of anoxia. A binational program has been initiated to conduct interdisciplinary, integrated impact assessments for the Great Lakes-St. Lawrence River Basin. The goal of this program is to undertake interdisciplinary, integrated studies to improve the understanding of the complex interactions between climate, the environment, and socioeconomic systems in order to develop informed regional adaptation responses

  9. The expression of miR-181a-5p and miR-371b-5p in chondrosarcoma.

    Science.gov (United States)

    Mutlu, S; Mutlu, H; Kirkbes, S; Eroglu, S; Kabukcuoglu, Y S; Kabukcuoglu, F; Duymus, T M; ISık, M; Ulasli, M

    2015-07-01

    Chondrosarcomas are malignant tumors of chondrocytes that affect bones and joints, and it represents the third most common type of primary bone tumors. Chondrosarcoma is difficult to treat because it is relatively resistant to both chemotherapy and radiation. Thus, surgery remains the best available treatment. It is important to find new diagnostic markers and improve treatment options. miRNAs are small non-coding transcripts (19-25 nucleotides) that regulate gene expression via targeting complementary sequences within messenger RNAs (mRNAs). miRNAs have been shown to be involved in regulation of many biochemical pathways. Dysregulated expression of many miRNAs has also been associated with multiple human diseases, such as cancer. 18 surgical chondrosarcoma specimens were obtained from patients. RNA extractions were performed from decalcified paraffin embedded tissues. The aim of this study was to investigate the expression levels of miR-181a and miR-371b in patients with chondrosarcoma by using RT-PCR and to evaluate the relationship between these miRNAs and chondrosarcoma. miR-181a was found to be upregulated in chondrosarcoma specimens whereas no significant alteration was found for miR-371b expression. It has been proposed that miRNA expression studies might be used as diagnostic, prognostic marker in cancer. miRNA expression data produced in our study may contribute future chondrosarcoma diagnosis and therapy.

  10. Integrating miRNA and mRNA Expression Profiling Uncovers miRNAs Underlying Fat Deposition in Sheep

    Directory of Open Access Journals (Sweden)

    Guangxian Zhou

    2017-01-01

    Full Text Available MicroRNAs (miRNAs are endogenous, noncoding RNAs that regulate various biological processes including adipogenesis and fat metabolism. Here, we adopted a deep sequencing approach to determine the identity and abundance of miRNAs involved in fat deposition in adipose tissues from fat-tailed (Kazakhstan sheep, KS and thin-tailed (Tibetan sheep, TS sheep breeds. By comparing HiSeq data of these two breeds, 539 miRNAs were shared in both breeds, whereas 179 and 97 miRNAs were uniquely expressed in KS and TS, respectively. We also identified 35 miRNAs that are considered to be putative novel miRNAs. The integration of miRNA-mRNA analysis revealed that miRNA-associated targets were mainly involved in the gene ontology (GO biological processes concerning cellular process and metabolic process, and miRNAs play critical roles in fat deposition through their ability to regulate fundamental pathways. These pathways included the MAPK signaling pathway, FoxO and Wnt signaling pathway, and focal adhesion. Taken together, our results define miRNA expression signatures that may contribute to fat deposition and lipid metabolism in sheep.

  11. ENDL-84. The Evaluated Nuclear Data Library of the Lawrence Livermore National Laboratory in the ENDF-5 format

    International Nuclear Information System (INIS)

    Cullen, D.E.; McLaughlin, P.K.; Lemmel, H.D.

    1990-09-01

    This document summarizes the contents of the evaluated nuclear data library (ENDL) by the Lawrence Livermore National Laboratory, USA, converted to ENDF-5 format. The library contains evaluated data for all significant neutron reactions in the energy range from 10 -4 eV to 20 MeV for 94 elements or isotopes. The entire library or selective retrievals from it can be obtained on magnetic tape, free of charge, from the IAEA Nuclear Data Section. (author)

  12. Hypoxia in the St. Lawrence Estuary: How a Coding Error Led to the Belief that "Physics Controls Spatial Patterns".

    Directory of Open Access Journals (Sweden)

    Daniel Bourgault

    Full Text Available Two fundamental sign errors were found in a computer code used for studying the oxygen minimum zone (OMZ and hypoxia in the Estuary and Gulf of St. Lawrence. These errors invalidate the conclusions drawn from the model, and call into question a proposed mechanism for generating OMZ that challenges classical understanding. The study in question is being cited frequently, leading the discipline in the wrong direction.

  13. Use of a krypton isotope for rapid ion changeover at the Lawrence Berkeley Laboratory 88-inch cyclotron

    Science.gov (United States)

    Soli, George A.; Nichols, Donald K.

    1989-01-01

    An isotope of krypton, Kr86, has been combined with a mix of Ar, Ne, and N ions at the electron cyclotron resonance (ECR) source, at the Lawrence Berkeley Laboratory cyclotron, to provide rapid ion changeover in Single Event Phenomena (SEP) testing. The new technique has been proved out successfully by a recent Jet Propulsion Laboratory (JPL) test in which it was found that there was no measurable contamination from other isotopes.

  14. A case-control study of malignant melanoma among Lawrence Livermore National Laboratory employees: A critical evaluation

    Energy Technology Data Exchange (ETDEWEB)

    Kupper, L.L.; Setzer, R.W.; Schwartzbaum, J.; Janis, J.

    1987-07-01

    This document reports on a reevaluation of data obtained in a previous report on occupational factors associated with the development of malignant melanomas at Lawrence Livermore National Laboratory. The current report reduces the number of these factors from five to three based on a rigorous statistical analysis of the original data. Recommendations include restructuring the original questionnaire and trying to contact more individuals that worked with volatile photographic chemicals. 17 refs., 7 figs., 22 tabs. (TEM)

  15. A case-control study of malignant melanoma among Lawrence Livermore National Laboratory employees: A critical evaluation

    International Nuclear Information System (INIS)

    Kupper, L.L.; Setzer, R.W.; Schwartzbaum, J.; Janis, J.

    1987-01-01

    This document reports on a reevaluation of data obtained in a previous report on occupational factors associated with the development of malignant melanomas at Lawrence Livermore National Laboratory. The current report reduces the number of these factors from five to three based on a rigorous statistical analysis of the original data. Recommendations include restructuring the original questionnaire and trying to contact more individuals that worked with volatile photographic chemicals. 17 refs., 7 figs., 22 tabs

  16. Lawrence Livermore National Laboratory Experimental Test Site, Site 300, Biological Review, January 1, 2009 through December 31, 2012

    Energy Technology Data Exchange (ETDEWEB)

    Paterson, Lisa E. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Woollett, Jim S. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2014-01-01

    The Lawrence Livermore National Laboratory’s (LLNL’s) Environmental Restoration Department (ERD) is required to conduct an ecological review at least every five years to ensure that biological and contaminant conditions in areas undergoing remediation have not changed such that existing conditions pose an ecological hazard (Dibley et al. 2009a). This biological review is being prepared by the Natural Resources Team within LLNL’s Environmental Functional Area (EFA) to support the 2013 five-year ecological review.

  17. Diet composition and fish consumption of double-crested cormorants from three St. Lawrence River Colonies in 2013

    Science.gov (United States)

    Johnson, James H.; Farquhar, James F.; Mazzocchi, Irene M.; Bendig, Anne

    2014-01-01

    Double-crested Cormorants (Phalacrocorax auritus) were first observed nesting in the upper St. Lawrence River at Strachan Island in 1992. Cormorants now nest at a number of islands in the Thousand Islands section of the river. Griswold, McNair, and Strachan islands are among the largest colonies in the upper river. Until 2011, nest counts had remained relatively stable, ranging from 200 to 603 nests per colony. However, since 2011 the number of nests at McNair Island have exceeded 700 each year. Although the size of cormorant colonies in the upper St. Lawrence River is smaller than those in the eastern basin of Lake Ontario, the close proximity of islands in the upper river that have colonies may cause a cumulative fish consumption effect similar to a larger colony. Because of increasing numbers of Double-crested Cormorants in the upper St. Lawrence River and the possible effects on fish populations, studies were initiated in 1999 to quantify cormorant diet and fish consumption at the three largest colonies. From 1999 to 2012, these studies have shown that cormorants consumed about 128.6 million fish including 37.5 million yellow perch (Perca flavescens), 17.4 million rock bass (Ambloplites rupestris) and 1.0 million smallmouth bass (Micropterus dolemieu) (Johnson et al. 2012). During this same time period fish assessment studies near some of these islands have shown a major decrease in yellow perch populations (Klindt 2007). This occurrence is known as the halo effect and happens when piscivorous birds deplete local fish populations in areas immediately surrounding the colony (Ashmole 1963). This paper describes the diet and fish consumption of cormorants in the upper St. Lawrence River in 2013.

  18. Ungusobaba [you are our father]: The life of an Anglican bishop, Lawrence Bekisisa Zulu (1937-2013†)

    OpenAIRE

    Mbaya, Henry

    2012-01-01

    This study seeks to document the role that Lawrence Bekisisa Zulu played in the Anglican Church in South Africa (ACSA), particularly in the dioceses of Zululand and Swaziland, as a bishop. It records the life story of Zulu as a leader whose gifts as a pastor, teacher and priest enriched the lives of many clergy and lay people. That Zulu was entrusted with leadership positions in three dioceses, also suggests the strength of his moral authority and spirituality. The study demonstrates how the ...

  19. Institute of Geophysics and Planetary Physics (IGPP), Lawrence Livermore National Laboratory (LLNL): Quinquennial report, November 14-15, 1996

    Energy Technology Data Exchange (ETDEWEB)

    Tweed, J.

    1996-10-01

    This Quinquennial Review Report of the Lawrence Livermore National Laboratory (LLNL) branch of the Institute for Geophysics and Planetary Physics (IGPP) provides an overview of IGPP-LLNL, its mission, and research highlights of current scientific activities. This report also presents an overview of the University Collaborative Research Program (UCRP), a summary of the UCRP Fiscal Year 1997 proposal process and the project selection list, a funding summary for 1993-1996, seminars presented, and scientific publications. 2 figs., 3 tabs.

  20. Estimating the mercury exposure dose in a population of migratory bird hunters in the St. Lawrence River region, Quebec, Canada

    International Nuclear Information System (INIS)

    Duchesne, J.-F.; Levesque, B.; Gauvin, Denis; Braune, Birgit; Gingras, Suzanne; Dewailly, E.

    2004-01-01

    St. Lawrence River hunters (Quebec, Canada) are exposed to the pollutants, especially mercury, that contaminate birds and fish. However, the health risks of this have remained unclear because of a lack of information about the hunters' duck, geese, and sportfish consumption habits. A nutritional survey was set up to characterize waterfowl and sportfish consumption in St. Lawrence River duck hunters and to estimate their daily exposure to mercury. During the winter of 2000, 512 hunters selected from the Canadian Wildlife Service database completed a self-administered questionnaire. Daily exposure to contaminants was measured using data from the Canadian Wildlife Service (waterfowl) and available data on St. Lawrence River sportfish. The annual average consumption was 7.5 meals of ducks and geese and 8.7 meals of sportfish. The daily exposure to mercury related to waterfowl consumption was below the Canadian tolerable daily intake (TDI) of 0.47 μg/kg body wt/day for all participants. The daily mercury intake associated with fish consumption was greater than the TDI in 2 duck hunters. The daily exposure to mercury was higher than the TDI in 4 participants when both waterfowl and fish consumption were combined. Our results suggest that fish consumption (especially freshwater fish) represents the main source of exposure to pollutants in duck hunters

  1. Organotins in zebra mussels (Dreissena polymorpha) and sediments of the Quebec City Harbour area of the St. Lawrence River.

    Science.gov (United States)

    Regoli, L; Chan, H M; de Lafontaine, Y; Mikaelian, I

    2001-07-01

    Toxic antifouling agents such as tributyltin (TBT) and triphenyltin (TPT) have been released in aquatic ecosystems through the use of antifouling paint applied to ship hulls, pleasure crafts and fish nets and these compounds can bioaccumulate in aquatic organisms. The purpose of this study was 1) to assess the extent of the distribution of organotins from a contaminated marina to the St. Lawrence River system by measuring organotin concentrations in zebra mussels (Dreissena polymorpha) and in sediments collected from 9 sites along the St. Lawrence River near Quebec City in July 1998, and 2) to examine the histopathological condition of zebra mussel tissues from these sites. TBT concentrations in zebra mussels were between 37 and 1078 ng Sn g(-1) wet weight, with the highest value found in the Bassin Louise marina. Elevated concentrations were also found in two other marinas. The concentrations decreased sharply to background levels just outside the marinas. All butyltins were detected in all sediments analysed, with highest values found in the Bassin Louise marina. Phenyltins were detected in three of the nine sites in low concentrations (zebra mussels. There was a significant correlation between TBT in sediments and mussels. Gonadal development of zebra mussels varied largely between sites, and was negatively associated to TBT levels in mussel tissue. This study shows that TBT contamination remains a problem in localised freshwater sectors of the St. Lawrence River.

  2. The role of miRNAs in endometrial cancer.

    Science.gov (United States)

    Vasilatou, Diamantina; Sioulas, Vasileios D; Pappa, Vasiliki; Papageorgiou, Sotirios G; Vlahos, Nikolaos F

    2015-01-01

    miRNAs are small noncoding RNAs that regulate gene expression at the post-transcriptional level. Since their discovery, miRNAs have been associated with every cell function including malignant transformation and metastasis. Endometrial cancer is the most common gynecologic malignancy. However, improvement should be made in interobserver agreement on histological typing and individualized therapeutic approaches. This article summarizes the role of miRNAs in endometrial cancer pathogenesis and treatment.

  3. Inhibition of 14q32 MicroRNAs miR-329, miR-487b, miR-494, and miR-495 Increases Neovascularization and Blood Flow Recovery After Ischemia

    DEFF Research Database (Denmark)

    Welten, S. M. J.; Bastiaansen, Ajnm; de Jong, R. C. M.

    2014-01-01

    in mice after single femoral artery ligation. Methods and Results: Gene silencing oligonucleotides (GSOs) were used to inhibit 4 14q32 microRNAs, miR-329, miR-487b, miR-494, and miR-495, 1 day before double femoral artery ligation. Blood flow recovery was followed by laser Doppler perfusion imaging. All 4...... GSOs clearly improved blood flow recovery after ischemia. Mice treated with GSO-495 or GSO-329 showed increased perfusion already after 3 days (30% perfusion versus 15% in control), and those treated with GSO-329 showed a full recovery of perfusion after 7 days (versus 60% in control). Increased...

  4. Isolation and Identification of miRNAs in Jatropha curcas

    Science.gov (United States)

    Wang, Chun Ming; Liu, Peng; Sun, Fei; Li, Lei; Liu, Peng; Ye, Jian; Yue, Gen Hua

    2012-01-01

    MicroRNAs (miRNAs) are small noncoding RNAs that play crucial regulatory roles by targeting mRNAs for silencing. To identify miRNAs in Jatropha curcas L, a bioenergy crop, cDNA clones from two small RNA libraries of leaves and seeds were sequenced and analyzed using bioinformatic tools. Fifty-two putative miRNAs were found from the two libraries, among them six were identical to known miRNAs and 46 were novel. Differential expression patterns of 15 miRNAs in root, stem, leave, fruit and seed were detected using quantitative real-time PCR. Ten miRNAs were highly expressed in fruit or seed, implying that they may be involved in seed development or fatty acids synthesis in seed. Moreover, 28 targets of the isolated miRNAs were predicted from a jatropha cDNA library database. The miRNA target genes were predicted to encode a broad range of proteins. Sixteen targets had clear BLASTX hits to the Uniprot database and were associated with genes belonging to the three major gene ontology categories of biological process, cellular component, and molecular function. Four targets were identified for JcumiR004. By silencing JcumiR004 primary miRNA, expressions of the four target genes were up-regulated and oil composition were modulated significantly, indicating diverse functions of JcumiR004. PMID:22419887

  5. Exosomes as miRNA Carriers: Formation–Function–Future

    Science.gov (United States)

    Yu, Xiaojie; Odenthal, Margarete; Fries, Jochen W. U.

    2016-01-01

    Exosomes, which are one of the smallest extracellular vesicles released from cells, have been shown to carry different nucleic acids, including microRNAs (miRNAs). miRNAs significantly regulate cell growth and metabolism by posttranscriptional inhibition of gene expression. The rapidly changing understanding of exosomes’ formation and function in delivering miRNAs from cell to cell has prompted us to review current knowledge in exosomal miRNA secretion mechanisms as well as possible therapeutic applications for personalized medicine. PMID:27918449

  6. Exosomes as miRNA Carriers: Formation–Function–Future

    Directory of Open Access Journals (Sweden)

    Xiaojie Yu

    2016-12-01

    Full Text Available Exosomes, which are one of the smallest extracellular vesicles released from cells, have been shown to carry different nucleic acids, including microRNAs (miRNAs. miRNAs significantly regulate cell growth and metabolism by posttranscriptional inhibition of gene expression. The rapidly changing understanding of exosomes’ formation and function in delivering miRNAs from cell to cell has prompted us to review current knowledge in exosomal miRNA secretion mechanisms as well as possible therapeutic applications for personalized medicine.

  7. Viruses and miRNAs: More Friends than Foes.

    Science.gov (United States)

    Bruscella, Patrice; Bottini, Silvia; Baudesson, Camille; Pawlotsky, Jean-Michel; Feray, Cyrille; Trabucchi, Michele

    2017-01-01

    There is evidence that eukaryotic miRNAs (hereafter called host miRNAs) play a role in the replication and propagation of viruses. Expression or targeting of host miRNAs can be involved in cellular antiviral responses. Most times host miRNAs play a role in viral life-cycles and promote infection through complex regulatory pathways. miRNAs can also be encoded by a viral genome and be expressed in the host cell. Viral miRNAs can share common sequences with host miRNAs or have totally different sequences. They can regulate a variety of biological processes involved in viral infection, including apoptosis, evasion of the immune response, or modulation of viral life-cycle phases. Overall, virus/miRNA pathway interaction is defined by a plethora of complex mechanisms, though not yet fully understood. This article review summarizes recent advances and novel biological concepts related to the understanding of miRNA expression, control and function during viral infections. The article also discusses potential therapeutic applications of this particular host-pathogen interaction.

  8. Assay reproducibility in clinical studies of plasma miRNA.

    Directory of Open Access Journals (Sweden)

    Jonathan Rice

    Full Text Available There are increasing reports of plasma miRNAs as biomarkers of human disease but few standards in methodologic reporting, leading to inconsistent data. We systematically reviewed plasma miRNA studies published between July 2013-June 2014 to assess methodology. Six parameters were investigated: time to plasma extraction, methods of RNA extraction, type of miRNA, quantification, cycle threshold (Ct setting, and methods of statistical analysis. We compared these data with a proposed standard methodologic technique. Beginning with initial screening for 380 miRNAs using microfluidic array technology and validation in an additional cohort of patients, we compared 11 miRNAs that exhibited differential expression between 16 patients with benign colorectal neoplasms (advanced adenomas and 16 patients without any neoplasm (controls. Plasma was isolated immediately, 12, 24, 48, or 72 h following phlebotomy. miRNA was extracted using two different techniques (Trizol LS with pre-amplification or modified miRNeasy. We performed Taqman-based RT-PCR assays for the 11 miRNAs with subsequent analyses using a variable Ct setting or a fixed Ct set at 0.01, 0.03, 0.05, or 0.5. Assays were performed in duplicate by two different operators. RNU6 was the internal reference. Systematic review yielded 74 manuscripts meeting inclusion criteria. One manuscript (1.4% documented all 6 methodological parameters, while < 5% of studies listed Ct setting. In our proposed standard technique, plasma extraction ≤12 h provided consistent ΔCt. miRNeasy extraction yielded higher miRNA concentrations and fewer non-expressed miRNAs compared to Trizol LS (1/704 miRNAs [0.14%] vs 109/704 miRNAs [15%], not expressed, respectively. A fixed Ct bar setting of 0.03 yielded the most reproducible data, provided that <10% miRNA were non-expressed. There was no significant intra-operator variability. There was significant inter-operator variation using Trizol LS extraction, while this was

  9. Koncept oblikovne zasnove multifunkcionalne miške

    OpenAIRE

    Jelenko, Matic

    2016-01-01

    Diplomsko delo opisuje oblikovno zasnovo multifunkcijske miške, katere oblika temelji na združitvi klasične računalniški miške ter 3D miške. Predstavljena je raziskava zgodovine računalniške miške ter proces razvoja od ideje do prototipa. Rezultat je fizični model realne velikosti, narejen z načinom hitre izdelave prototipov. Dodana vrednost je dosežena z implikacijo grafično dekorativne podobe.

  10. Tissue-dependent paired expression of miRNAs

    OpenAIRE

    Ro, Seungil; Park, Chanjae; Young, David; Sanders, Kenton M.; Yan, Wei

    2007-01-01

    It is believed that depending on the thermodynamic stability of the 5′-strand and the 3′-strand in the stem-loop structure of a precursor microRNA (pre-miRNA), cells preferentially select the less stable one (called the miRNA or guide strand) and destroy the other one (called the miRNA* or passenger strand). However, our expression profiling analyses revealed that both strands could be co-accumulated as miRNA pairs in some tissues while being subjected to strand selection in other tissues. Ou...

  11. Methylation of miRNA genes and oncogenesis.

    Science.gov (United States)

    Loginov, V I; Rykov, S V; Fridman, M V; Braga, E A

    2015-02-01

    Interaction between microRNA (miRNA) and messenger RNA of target genes at the posttranscriptional level provides fine-tuned dynamic regulation of cell signaling pathways. Each miRNA can be involved in regulating hundreds of protein-coding genes, and, conversely, a number of different miRNAs usually target a structural gene. Epigenetic gene inactivation associated with methylation of promoter CpG-islands is common to both protein-coding genes and miRNA genes. Here, data on functions of miRNAs in development of tumor-cell phenotype are reviewed. Genomic organization of promoter CpG-islands of the miRNA genes located in inter- and intragenic areas is discussed. The literature and our own results on frequency of CpG-island methylation in miRNA genes from tumors are summarized, and data regarding a link between such modification and changed activity of miRNA genes and, consequently, protein-coding target genes are presented. Moreover, the impact of miRNA gene methylation on key oncogenetic processes as well as affected signaling pathways is discussed.

  12. Supplement analysis for Greenville Gate access to Kirschbaum Field at Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    1997-01-01

    The National Ignition Facility (NIF) Program proposes to provide additional access to the Kirschbaum Field construction laydown area. This additional access would alleviate traffic congestion at the East Gate entrance to Lawrence Livermore National Laboratory (LLNL) from Greenville Road during periods of heavy construction for the NIF. The new access would be located along the northeastern boundary of LLNL, about 305 m (1,000 ft) north of the East Gate entrance. The access road would extend from Greenville Road to the Kirschbaum Field construction laydown area and would traverse an existing storm water drainage channel. Two culverts, side by side, and a compacted road base would be installed across the channel. The security fence that runs parallel to Greenville Road would be modified to accommodate this new entrance and a vehicle gate would be installed at the entrance of Kirschbaum Field. The exiting shoulder along Greenville Road would be converted into a new turn lane for trucks entering the new gate. This analysis evaluates the impacts of constructing the Kirschbaum Field bridge and access gate at a different location than was analyzed in the NIF Project specific Analysis in the Final Programmatic environmental Impact Statement for Stockpile Stewardship and Management (SS and M PEIS) published in September 1996 (DOE/EIS-0236) and the Record of Decision published on December 19, 1996. Issues of concern addressed in this supplement analysis include potential impacts to wetlands downstream of the access bridge, potential impacts to the California red-legged frog (Rana aurora draytonii) listed as threatened on the federal listing pursuant to the Endangered Species Act of 1974, and potential impacts on the 100-yr floodplain along the Arroyo Las Positas

  13. Chemical health risk assessment for hazardous and mixed waste management units at Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    1992-09-01

    The Lawrence Livermore National Laboratory (LLNL) operates three Hazardous Waste Management Facilities with 24 associated waste management units for the treatment and storage of hazardous and mixed wastes. These wastes are generated by research programs and support operations. The storage and treatment units are presently operated under interim status in accordance with the requirements of the US Envirorunental Protection Agency (US EPA) and the Department of Toxic Substances Control (DTSC), a division of the California Envirorunental Protection Agency (Cal/EPA). As required by the California Hazardous Waste Control Act and the Resource Conservation and Recovery Act (RCRA), LLNL ha s applied for a Part B permit to continue operating the storage and waste treatment facilities. As part of this permitting process, LLNL is required to conduct a health risk assessment (HRA) to examine the potential health impacts to the surrounding community from continued storage and treatment of hazardous and mixed wastes. analysis document presents the results of this risk assessment. An analysis of maximum credible chemical accidents is also included in Section 7.0. This HRA was prepared in accordance with procedures set forth by the California Air Pollution Control Officers Association (CAPCOA) ''Air Toxics Assessment Manual,'' CAPCOA guidelines for preparing risk assessments under the Air Toxic ''Hot Spots'' Act (AB 2588) and requirements of the US EPA. By following these procedures, this risk assessment presents a conservative analysis of a hypothetical Maximally Exposed Individual (MEI) using many worst-case assumptions that will not apply to an actual individual. As such, the risk estimates presented should be regarded as a worst-case estimate of any actual risk that may be present

  14. Potential for saturated ground-water system contamination at the Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    Stone, R.; Ruggieri, M.R.; Rogers, L.L.; Emerson, D.O.; Buddemeier, R.W.

    1982-01-01

    A program of hydrogeologic investigation has been carried out to determine the likelihood of contaminant movement to the saturated zone from near the ground surface at Lawrence Livermore National Laboratory (LLNL). A companion survey of potential contaminant sources was also conducted at the LLNL. Water samples from selected LLNL wells were analyzed to test the water quality in the uppermost part of the saturated zone, which is from 14 to 48 m (45 to 158 ft) beneath the surface. Only nitrate and tritium were found in concentrations above natural background. In one well, the nitrate was slightly more concentrated than the drinking water limit. The nitrate source has not been found. The tritium in all ground-water samples from wells was found far less concentrated than the drinking water limit. The extent of infiltration of surface water was traced with environmental tritium. The thickness and stratigraphy of the unsaturated zone beneath the LLNL, and nearby area, was determined with specially constructed wells and boreholes. Well hydrograph analysis indicated where infiltration of surface water reached the saturated ground-water system. The investigation indicates that water infiltrating from the surface, through alluvial deposits, reaches the saturated zone along the course of Arroyo Seco, Arroyo Las Positas, and from the depression near the center of the site where seasonal water accumulates. Several potential contaminant sources were identified, and it is likely that contaminants could move from near the ground surface to the saturated zone beneath LLNL. Additional ground-water sampling and analysis will be performed and ongoing investigations will provide estimates of the speed with which potential contaminants can flow laterally in the saturated zone beneath LLNL. 34 references, 61 figures, 16 tables

  15. A Rose By Other Names: Some General Musings on Lawrence and Colleagues' Hidden Curriculum Scoping Review.

    Science.gov (United States)

    Hafferty, Frederic W; Martimianakis, Maria Athina

    2017-11-07

    In this Commentary, the authors explore the scoping review by Lawrence and colleagues by challenging their conclusion that with over 25 years' worth of "ambiguous and seemingly ubiquitous use" of the hidden curriculum construct in health professions education scholarship, it is time to either move to a more uniform definitional foundation or abandon the term altogether. The commentary authors counter these remedial propositions by foregrounding the importance of theoretical diversity and the conceptual richness afforded when the hidden curriculum construct is used as an entry point for studying the interstitial space between the formal and a range of other-than-formal domains of learning. Further, they document how tightly-delimited scoping strategies fail to capture the wealth of educational scholarship that operates within a hidden curriculum framework, including "hidden" hidden curriculum articles, studies that employ alternative constructs, and investigations that target important tacit socio-cultural influences on learners and faculty without formally deploying the term. They offer examples of how the hidden curriculum construct, while undergoing significant transformation in its application within the field of health professions education, has created the conceptual foundation for the application of a number of critical perspectives that make visible the field's political investments in particular forms of knowing and associated practices. Finally, the commentary authors invite readers to consider the methodological promise afforded by conceptual heterogeneity, particularly strands of scholarship that resituate the hidden curriculum concept within the magically expansive dance of social relationships, social learning, and social life that form the learning environments of health professions education.

  16. Social and technical history of the electrification of the lower Saint Lawrence, 1888-1963

    International Nuclear Information System (INIS)

    Tremblay, Y.

    1993-06-01

    The lower St. Lawrence region of Quebec is a rural area with little industry and does not possess important hydraulic resources. Electrification of this region progressed slowly and in an uneven manner. From 1888 to 1922, many local distributors, private or municipal, gave a mediocre quality of service to a small number of customers. A regional firm, the Compagnie de Pouvoir du Bas St-Laurent, followed in 1922 but its resources remained limited, and in a few years it passed under the control of American capitalists. The most rural sectors of the region were badly provided with electricity even in 1945, and the state, in the form of the Office de l'Electrification Rurale, had to intervene to remedy this situation. The Office took charge of organizing and financing electric power distribution cooperatives which ensured service in the most remote zones of Quebec. At the beginning of the 1960s, the inequalities in electric power rates in the region supplied a weighty argument to those in favor of nationalization of power companies. The Compagnie de Pouvoir was acquired by Hydro-Quebec in 1963 and the region was finally correctly integrated with the provincial grid. The cooperatives were also acquired by the Crown corporation a short time thereafter. Electrification was not only building power plants and distribution networks, but also involved development of consumption of electricity on farms and in homes. This made city comforts universal and modified rural life styles, for example by the introduction of electric appliances. 430 refs., 35 figs., 70 tabs

  17. Visibility of St Lawrence belugas to aerial photography, estimated by direct observation

    Directory of Open Access Journals (Sweden)

    Michael CS Kingsley

    2002-07-01

    Full Text Available The depleted population of belugas (Delphinapterus leucas inhabiting the St Lawrence estuary, Canada, was monitored by periodic photographic aerial surveys. In order to correct counts made on aerial survey film and to obtain an estimate of the true size of the population, the diving behaviour and the visibility from the air of these animals was studied. A Secchi-disk turbidity survey in the belugas’ summer range showed that water clarity varied between 1.5 m and 11.6 m. By studying aerial photographs of sheet-plastic models of belugas that had been sunk to different depths below the surface, we found that models of white adults could be seen down to about the same depth as a Secchi disk, but no deeper. Smaller models of dark-grey juveniles could only be seen down to about 50% of Secchi-disk depth. By observing groups of belugas from a hovering helicopter and recording their disappearances and re-appearances, it was found that they were visible for 44.3% of the time, and that an appropriate correction for single photographs would be to multiply the photographic count by about 222% (SE 20%. For surveys in which there was overlap between adjacent frames, the estimated correction would be 209% (SE 16%. This correction factor was slightly conservative and gave an estimate of the true size of the population, based on a single survey, of 1,202 belugas (SE 189 in 1997. An estimate for 1997 based on smoothing 5 surveys 1988–1997 was 1,238 (SE 119.

  18. Public census data on CD-ROM at Lawrence Berkeley Laboratory. Revision 4

    Energy Technology Data Exchange (ETDEWEB)

    Merrill, D.W.

    1993-03-12

    The Comprehensive Epidemiologic Data Resource (CEDR) and Populations at Risk to Environmental Pollution (PAREP) projects, of the Information and Computing sciences Division (ICSD) at Lawrence Berkeley Laboratory (LBL), are using public socioeconomic and geographic data files which are available to CEDR and PAREP collaborators via LBL`s computing network. At this time 89 CD-ROM diskettes (approximately 45 gigabytes) are on line via the Unix file server cedrcd.lbl.gov. Most of the files are from the US Bureau of the Census, and many of these pertain to the 1990 Census of Population and Housing. All the CD-ROM diskettes contain documentation in the form of ASCII text files. In addition, printed documentation for most files is available for inspection at University of California Data and Technical Assistance (UC DATA), tel. (510) 642-6571, or the UC Documents Library, tel. (510) 642-2569, both located on the UC Berkeley Campus. Many of the CD-ROM diskettes distributed by the Census Bureau contain software for PC compatible computers, for easily accessing the data. Shared access to the data is maintained through a collaboration among the CEDR and PAREP projects at LBL, and UC DATA, and the UC Documents Library. LBL is grateful to UC DATA and the UC Documents Library for the use of their CD-ROM diskettes. Shared access to LBL facilities may be restricted in the future if costs become prohibitive. Via the Sun Network File System (NFS), these data can be exported to Internet computers for direct access by the user`s application program(s). Due to the size of the files, this access method is preferred over File Transfer Protocol (FTP) access.

  19. Public census data on CD-ROM at Lawrence Berkeley Laboratory. Revision 3

    Energy Technology Data Exchange (ETDEWEB)

    Merrill, D.W.

    1993-01-16

    The Comprehensive Epidemiologic Data Resource (CEDR) and Populations at Risk to Environmental Pollution (PAREP) projects, of the Information and Computing Sciences Division (ICSD) at Lawrence Berkeley Laboratory (LBL), are using public socioeconomic and geographic data files which are available to CEDR and PAREP collaborators via LBL`s computing network. At this time 72 CD-ROM diskettes (approximately 37 gigabytes) are on line via the Unix file server ``cedrcd.lbl.gov``. Most of the files are from the US Bureau of the Census, and many of these pertain to the 1990 Census of Population and Housing. All the CD-ROM diskettes contain documentation in the form of ASCII text files. In addition, printed documentation for most files is available for inspection at University of California Data and Technical Assistance (UC DATA), tel. (510) 642-6571, or the UC Documents Library, tel. (510) 642-2569, both located on the UC Berkeley Campus. Many of the CD-ROM diskettes distributed by the Census Bureau contain software for PC compatible computers, for easily accessing the data. Shared access to the data is maintained through a collaboration among the CEDR and PAREP projects at LBL, and UC DATA, and the UC Documents Library. LBL is grateful to UC DATA and the UC Documents Library for the use of their CD-ROM diskettes. Shared access to LBL facilities may be restricted in the future if costs become prohibitive. Via the Sun Network File System (NFS), these data can be exported to Internet computers for direct access by the user`s application program(s). Due to the size of the files, this access method is preferred over File Transfer Protocol (FTP) access. Please contact Deane Merrill (dwmerrill@lbl.gov) if you wish to make use of the data.

  20. Public census data on CD-ROM at Lawrence Berkeley Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Merrill, D.W.

    1993-01-16

    The Comprehensive Epidemiologic Data Resource (CEDR) and Populations at Risk to Environmental Pollution (PAREP) projects, of the Information and Computing Sciences Division (ICSD) at Lawrence Berkeley Laboratory (LBL), are using public socioeconomic and geographic data files which are available to CEDR and PAREP collaborators via LBL's computing network. At this time 72 CD-ROM diskettes (approximately 37 gigabytes) are on line via the Unix file server cedrcd.lbl.gov''. Most of the files are from the US Bureau of the Census, and many of these pertain to the 1990 Census of Population and Housing. All the CD-ROM diskettes contain documentation in the form of ASCII text files. In addition, printed documentation for most files is available for inspection at University of California Data and Technical Assistance (UC DATA), tel. (510) 642-6571, or the UC Documents Library, tel. (510) 642-2569, both located on the UC Berkeley Campus. Many of the CD-ROM diskettes distributed by the Census Bureau contain software for PC compatible computers, for easily accessing the data. Shared access to the data is maintained through a collaboration among the CEDR and PAREP projects at LBL, and UC DATA, and the UC Documents Library. LBL is grateful to UC DATA and the UC Documents Library for the use of their CD-ROM diskettes. Shared access to LBL facilities may be restricted in the future if costs become prohibitive. Via the Sun Network File System (NFS), these data can be exported to Internet computers for direct access by the user's application program(s). Due to the size of the files, this access method is preferred over File Transfer Protocol (FTP) access. Please contact Deane Merrill (dwmerrill lbl.gov) if you wish to make use of the data.

  1. Environmental impact report addendum for the continued operation of Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    Weston, R. F.

    1996-01-01

    An environmental impact statement/environmental impact report (ES/EIR) for the continued operation and management of Lawrence Livermore National Laboratory (LLNL) was prepared jointly by the U.S. Department of Energy (DOE) and the University of California (UC). The scope of the document included near-term (within 5-10 years) proposed projects. The UC Board of Regents, as state lead agency under the California Environmental Quality Act (CEQA), certified and adopted the EIR by issuing a Notice of Determination on November 20, 1992. The DOE, as the lead federal agency under the National Environmental Policy Act (NEPA), adopted a Record of Decision for the ES on January 27, 1993 (58 Federal Register [FR] 6268). The DOE proposed action was to continue operation of the facility, including near-term proposed projects. The specific project evaluated by UC was extension of the contract between UC and DOE for UC's continued operation and management of LLNL (both sites) from October 1, 1992, through September 30, 1997. The 1992 ES/EIR analyzed impacts through the year 2002. The 1992 ES/EIR comprehensively evaluated the potential environmental impacts of operation and management of LLNL within the near-term future. Activities evaluated included programmatic enhancements and modifications of facilities and programs at the LLNL Livermore site and at LLNL's Experimental Test Site (Site 300) in support of research and development missions 2048 established for LLNL by Congress and the President. The evaluation also considered the impacts of infrastructure and building maintenance, minor modifications to buildings, general landscaping, road maintenance, and similar routine support activities

  2. Genome-wide miRNA screening reveals miR-310 family members negatively regulate the immune response in Drosophila melanogaster via co-targeting Drosomycin.

    Science.gov (United States)

    Li, Yao; Li, Shengjie; Li, Ruimin; Xu, Jiao; Jin, Ping; Chen, Liming; Ma, Fei

    2017-03-01

    Although innate immunity mediated by Toll signaling has been extensively studied in Drosophila melanogaster, the role of miRNAs in regulating the Toll-mediated immune response remains largely unknown. In this study, following Gram-positive bacterial challenge, we identified 93 differentially expressed miRNAs via genome-wide miRNA screening. These miRNAs were regarded as immune response related (IRR). Eight miRNAs were confirmed to be involved in the Toll-mediated immune response upon Gram-positive bacterial infection through genetic screening of 41 UAS-miRNA lines covering 60 miRNAs of the 93 IRR miRNAs. Interestingly, four out of these eight miRNAs, miR-310, miR-311, miR-312 and miR-313, are clustered miRNAs and belong to the miR-310 family. These miR-310 family members were shown to target and regulate the expression of Drosomycin, an antimicrobial peptide produced by Toll signaling. Taken together, our study implies important regulatory roles of miRNAs in the Toll-mediated innate immune response of Drosophila upon Gram-positive bacterial infection. Copyright © 2016 Elsevier Ltd. All rights reserved.

  3. Targeting oncomiRNAs and mimicking tumor suppressor miRNAs: New trends in the development of miRNA therapeutic strategies in oncology (Review)

    Science.gov (United States)

    GAMBARI, ROBERTO; BROGNARA, ELEONORA; SPANDIDOS, DEMETRIOS A.; FABBRI, ENRICA

    2016-01-01

    MicroRNA (miRNA or miR) therapeutics in cancer are based on targeting or mimicking miRNAs involved in cancer onset, progression, angiogenesis, epithelial-mesenchymal transition and metastasis. Several studies conclusively have demonstrated that miRNAs are deeply involved in tumor onset and progression, either behaving as tumor-promoting miRNAs (oncomiRNAs and metastamiRNAs) or as tumor suppressor miRNAs. This review focuses on the most promising examples potentially leading to the development of anticancer, miRNA-based therapeutic protocols. The inhibition of miRNA activity can be readily achieved by the use of miRNA inhibitors and oligomers, including RNA, DNA and DNA analogues (miRNA antisense therapy), small molecule inhibitors, miRNA sponges or through miRNA masking. On the contrary, the enhancement of miRNA function (miRNA replacement therapy) can be achieved by the use of modified miRNA mimetics, such as plasmid or lentiviral vectors carrying miRNA sequences. Combination strategies have been recently developed based on the observation that i) the combined administration of different antagomiR molecules induces greater antitumor effects and ii) some anti-miR molecules can sensitize drug-resistant tumor cell lines to therapeutic drugs. In this review, we discuss two additional issues: i) the combination of miRNA replacement therapy with drug administration and ii) the combination of antagomiR and miRNA replacement therapy. One of the solid results emerging from different independent studies is that miRNA replacement therapy can enhance the antitumor effects of the antitumor drugs. The second important conclusion of the reviewed studies is that the combination of anti-miRNA and miRNA replacement strategies may lead to excellent results, in terms of antitumor effects. PMID:27175518

  4. miR398 and miR395 are involved in response to SO2 stress in Arabidopsis thaliana.

    Science.gov (United States)

    Li, Lihong; Yi, Huilan; Xue, Meizhao; Yi, Min

    2017-11-01

    Sulfur dioxide (SO 2 ) is a common air pollutant that has adverse effects on plants. MicroRNAs (miRNAs) are small noncoding RNA that play critical roles in plant development and stress response. In this study, we found that two miRNAs, miR398 and miR395, were differentially expressed in Arabidopsis shoots under SO 2 stress. The expression of miR398 was down-regulated, and the transcript levels of its target genes, Cu/Zn superoxide dismutases (CSD1 and CSD2), were increased during SO 2 exposure. The activity of superoxide dismutase (SOD), one of the major antioxidant enzymes, was enhanced with the increase in the CSD transcript level, suggesting an important role of miR398 in response to SO 2 -induced oxidative stress. Meanwhile, the expression of miR395 was increased, and the transcript levels of its target genes, ATP sulfurylases (APS3 and APS4) and a low-affinity sulfate transporter (SULTR2;1), were decreased in Arabidopsis shoots, showing that miR395 played important roles in the regulation of sulfate assimilation and translocation during SO 2 exposure. The content of glutathione (GSH), an important sulfur-containing antioxidant, was enhanced with the changes in sulfur metabolism in Arabidopsis shoots under SO 2 stress. These results showed that both miR398 and miR395 were involved in protecting plants from oxidative damage during SO 2 exposure. Many stress-responsive cis-elements were found in the promoter regions of MIR398 and MIR395, suggesting that these miRNAs might respond to various environmental conditions, including SO 2 stress. Overall, our study provides an insight into the regulatory roles of miRNAs in response to SO 2 stress in plants, and highlights the molecular mechanisms of plant adaptation to environmental stress.

  5. Expression patterns of miR-146a and miR-146b in mastitis infected dairy cattle.

    Science.gov (United States)

    Wang, Xing Ping; Luoreng, Zhuo Ma; Zan, Lin Sen; Raza, Sayed Haidar Abbas; Li, Feng; Li, Na; Liu, Shuan

    2016-10-01

    This study reports a significant up-regulation of bta-miR-146a and bta-miR-146b expression levels in bovine mammary tissues infected with subclinical, clinical and experimental mastitis. Potential target genes are involved in multiple immunological pathways. These results suggest a regulatory function of both miRNAs for the bovine inflammatory response in mammary tissue. Copyright © 2016 Elsevier Ltd. All rights reserved.

  6. miRNA Expression Profile after Status Epilepticus and Hippocampal Neuroprotection by Targeting miR-132

    Science.gov (United States)

    Jimenez-Mateos, Eva M.; Bray, Isabella; Sanz-Rodriguez, Amaya; Engel, Tobias; McKiernan, Ross C.; Mouri, Genshin; Tanaka, Katsuhiro; Sano, Takanori; Saugstad, Julie A.; Simon, Roger P.; Stallings, Raymond L.; Henshall, David C.

    2011-01-01

    When an otherwise harmful insult to the brain is preceded by a brief, noninjurious stimulus, the brain becomes tolerant, and the resulting damage is reduced. Epileptic tolerance develops when brief seizures precede an episode of prolonged seizures (status epilepticus). MicroRNAs (miRNAs) are small, noncoding RNAs that function as post-transcriptional regulators of gene expression. We investigated how prior seizure preconditioning affects the miRNA response to status epilepticus evoked by intra-amygdalar kainic acid in mice. The miRNA was extracted from the ipsilateral CA3 subfield 24 hours after focal-onset status epilepticus in animals that had previously received either seizure preconditioning (tolerance) or no preconditioning (injury), and mature miRNA levels were measured using TaqMan low-density arrays. Expression of 21 miRNAs was increased, relative to control, after status epilepticus alone, and expression of 12 miRNAs was decreased. Increased miR-132 levels were matched with increased binding to Argonaute-2, a constituent of the RNA-induced silencing complex. In tolerant animals, expression responses of >40% of the injury-group-detected miRNAs differed, being either unchanged relative to control or down-regulated, and this included miR-132. In vivo microinjection of locked nucleic acid-modified oligonucleotides (antagomirs) against miR-132 depleted hippocampal miR-132 levels and reduced seizure-induced neuronal death. Thus, our data strongly suggest that miRNAs are important regulators of seizure-induced neuronal death. PMID:21945804

  7. Post-transcriptional generation of miRNA variants by multiple nucleotidyl transferases contributes to miRNA transcriptome complexity

    OpenAIRE

    Wyman, Stacia K.; Knouf, Emily C.; Parkin, Rachael K.; Fritz, Brian R.; Lin, Daniel W.; Dennis, Lucas M.; Krouse, Michael A.; Webster, Philippa J.; Tewari, Muneesh

    2011-01-01

    Modification of microRNA sequences by the 3′ addition of nucleotides to generate so-called “isomiRs” adds to the complexity of miRNA function, with recent reports showing that 3′ modifications can influence miRNA stability and efficiency of target repression. Here, we show that the 3′ modification of miRNAs is a physiological and common post-transcriptional event that shows selectivity for specific miRNAs and is observed across species ranging from C. elegans to human. The modifications resul...

  8. CID-miRNA: A web server for prediction of novel miRNA precursors in human genome

    International Nuclear Information System (INIS)

    Tyagi, Sonika; Vaz, Candida; Gupta, Vipin; Bhatia, Rohit; Maheshwari, Sachin; Srinivasan, Ashwin; Bhattacharya, Alok

    2008-01-01

    microRNAs (miRNA) are a class of non-protein coding functional RNAs that are thought to regulate expression of target genes by direct interaction with mRNAs. miRNAs have been identified through both experimental and computational methods in a variety of eukaryotic organisms. Though these approaches have been partially successful, there is a need to develop more tools for detection of these RNAs as they are also thought to be present in abundance in many genomes. In this report we describe a tool and a web server, named CID-miRNA, for identification of miRNA precursors in a given DNA sequence, utilising secondary structure-based filtering systems and an algorithm based on stochastic context free grammar trained on human miRNAs. CID-miRNA analyses a given sequence using a web interface, for presence of putative miRNA precursors and the generated output lists all the potential regions that can form miRNA-like structures. It can also scan large genomic sequences for the presence of potential miRNA precursors in its stand-alone form. The web server can be accessed at (http://mirna.jnu.ac.in/cidmirna/)

  9. Measurement of the Single Top Quark Production Cross Section and |<mi>Vmi><mi>tb>| in Events with One Charged Lepton, Large Missing Transverse Energy, and Jets at CDF

    Energy Technology Data Exchange (ETDEWEB)

    Aaltonen, T.; Amerio, S.; Amidei, D.; Anastassov, A.; Annovi, A.; Antos, J.; Apollinari, G.; Appel, J. A.; Arisawa, T.; Artikov, A.; Asaadi, J.; Ashmanskas, W.; Auerbach, B.; Aurisano, A.; Azfar, F.; Badgett, W.; Bae, T.; Barbaro-Galtieri, A.; Barnes, V. E.; Barnett, B. A.; Barria, P.; Bartos, P.; Bauce, M.; Bedeschi, F.; Behari, S.; Bellettini, G.; Bellinger, J.; Benjamin, D.; Beretvas, A.; Bhatti, A.; Bland, K. R.; Blumenfeld, B.; Bocci, A.; Bodek, A.; Bortoletto, D.; Boudreau, J.; Boveia, A.; Brigliadori, L.; Bromberg, C.; Brucken, E.; Budagov, J.; Budd, H. S.; Burkett, K.; Busetto, G.; Bussey, P.; Butti, P.; Buzatu, A.; Calamba, A.; Camarda, S.; Campanelli, M.; Canelli, F.; Carls, B.; Carlsmith, D.; Carosi, R.; Carrillo, S.; Casal, B.; Casarsa, M.; Castro, A.; Catastini, P.; Cauz, D.; Cavaliere, V.; Cerri, A.; Cerrito, L.; Chen, Y. C.; Chertok, M.; Chiarelli, G.; Chlachidze, G.; Cho, K.; Chokheli, D.; Clark, A.; Clarke, C.; Convery, M. E.; Conway, J.; Corbo, M.; Cordelli, M.; Cox, C. A.; Cox, D. J.; Cremonesi, M.; Cruz, D.; Cuevas, J.; Culbertson, R.; d’Ascenzo, N.; Datta, M.; de Barbaro, P.; Demortier, L.; Deninno, M.; D’Errico, M.; Devoto, F.; Di Canto, A.; Di Ruzza, B.; Dittmann, J. R.; Donati, S.; D’Onofrio, M.; Dorigo, M.; Driutti, A.; Ebina, K.; Edgar, R.; Elagin, A.; Erbacher, R.; Errede, S.; Esham, B.; Farrington, S.; Fernández Ramos, J. P.; Field, R.; Flanagan, G.; Forrest, R.; Franklin, M.; Freeman, J. C.; Frisch, H.; Funakoshi, Y.; Galloni, C.; Garfinkel, A. F.; Garosi, P.; Gerberich, H.; Gerchtein, E.; Giagu, S.; Giakoumopoulou, V.; Gibson, K.; Ginsburg, C. M.; Giokaris, N.; Giromini, P.; Glagolev, V.; Glenzinski, D.; Gold, M.; Goldin, D.; Golossanov, A.; Gomez, G.; Gomez-Ceballos, G.; Goncharov, M.; González López, O.; Gorelov, I.; Goshaw, A. T.; Goulianos, K.; Gramellini, E.; Grosso-Pilcher, C.; Group, R. C.; Guimaraes da Costa, J.; Hahn, S. R.; Han, J. Y.; Happacher, F.; Hara, K.; Hare, M.; Harr, R. F.; Harrington-Taber, T.; Hatakeyama, K.; Hays, C.; Heinrich, J.; Herndon, M.; Hirschbuehl, D.; Hocker, A.; Hong, Z.; Hopkins, W.; Hou, S.; Hughes, R. E.; Husemann, U.; Hussein, M.; Huston, J.; Introzzi, G.; Iori, M.; Ivanov, A.; James, E.; Jang, D.; Jayatilaka, B.; Jeon, E. J.; Jindariani, S.; Jones, M.; Joo, K. K.; Jun, S. Y.; Junk, T. R.; Kambeitz, M.; Kamon, T.; Karchin, P. E.; Kasmi, A.; Kato, Y.; Ketchum, W.; Keung, J.; Kilminster, B.; Kim, D. H.; Kim, H. S.; Kim, J. E.; Kim, M. J.; Kim, S. H.; Kim, S. B.; Kim, Y. J.; Kim, Y. K.; Kimura, N.; Kirby, M.; Knoepfel, K.; Kondo, K.; Kong, D. J.; Konigsberg, J.; Kotwal, A. V.; Kreps, M.; Kroll, J.; Kruse, M.; Kuhr, T.; Kurata, M.; Laasanen, A. T.; Lammel, S.; Lancaster, M.; Lannon, K.; Latino, G.; Lee, H. S.; Lee, J. S.; Leo, S.; Leone, S.; Lewis, J. D.; Limosani, A.; Lipeles, E.; Lister, A.; Liu, H.; Liu, Q.; Liu, T.; Lockwitz, S.; Loginov, A.; Lucchesi, D.; Lucà, A.; Lueck, J.; Lujan, P.; Lukens, P.; Lungu, G.; Lys, J.; Lysak, R.; Madrak, R.; Maestro, P.; Malik, S.; Manca, G.; Manousakis-Katsikakis, A.; Marchese, L.; Margaroli, F.; Marino, P.; Matera, K.; Mattson, M. E.; Mazzacane, A.; Mazzanti, P.; McNulty, R.; Mehta, A.; Mehtala, P.; Mesropian, C.; Miao, T.; Mietlicki, D.; Mitra, A.; Miyake, H.; Moed, S.; Moggi, N.; Moon, C. S.; Moore, R.; Morello, M. J.; Mukherjee, A.; Muller, Th.; Murat, P.; Mussini, M.; Nachtman, J.; Nagai, Y.; Naganoma, J.; Nakano, I.; Napier, A.; Nett, J.; Neu, C.; Nigmanov, T.; Nodulman, L.; Noh, S. Y.; Norniella, O.; Oakes, L.; Oh, S. H.; Oh, Y. D.; Oksuzian, I.; Okusawa, T.; Orava, R.; Ortolan, L.; Pagliarone, C.; Palencia, E.; Palni, P.; Papadimitriou, V.; Parker, W.; Pauletta, G.; Paulini, M.; Paus, C.; Phillips, T. J.; Pianori, E.; Pilot, J.; Pitts, K.; Plager, C.; Pondrom, L.; Poprocki, S.; Potamianos, K.; Pranko, A.; Prokoshin, F.; Ptohos, F.; Punzi, G.; Redondo Fernández, I.; Renton, P.; Rescigno, M.; Rimondi, F.; Ristori, L.; Robson, A.; Rodriguez, T.; Rolli, S.; Ronzani, M.; Roser, R.; Rosner, J. L.; Ruffini, F.; Ruiz, A.; Russ, J.; Rusu, V.; Sakumoto, W. K.; Sakurai, Y.; Santi, L.; Sato, K.; Saveliev, V.; Savoy-Navarro, A.; Schlabach, P.; Schmidt, E. E.; Schwarz, T.; Scodellaro, L.; Scuri, F.; Seidel, S.; Seiya, Y.; Semenov, A.; Sforza, F.; Shalhout, S. Z.; Shears, T.; Shepard, P. F.; Shimojima, M.; Shochet, M.; Shreyber-Tecker, I.; Simonenko, A.; Sliwa, K.; Smith, J. R.; Snider, F. D.; Song, H.; Sorin, V.; St. Denis, R.; Stancari, M.; Stentz, D.; Strologas, J.; Sudo, Y.; Sukhanov, A.; Suslov, I.; Takemasa, K.; Takeuchi, Y.; Tang, J.; Tecchio, M.; Teng, P. K.; Thom, J.; Thomson, E.; Thukral, V.; Toback, D.; Tokar, S.; Tollefson, K.; Tomura, T.; Tonelli, D.; Torre, S.; Torretta, D.; Totaro, P.; Trovato, M.; Ukegawa, F.; Uozumi, S.; Vázquez, F.; Velev, G.; Vellidis, C.; Vernieri, C.; Vidal, M.; Vilar, R.; Vizán, J.; Vogel, M.; Volpi, G.; Wagner, P.; Wallny, R.; Wang, S. M.; Waters, D.; Wester, W. C.; Whiteson, D.; Wicklund, A. B.; Wilbur, S.; Williams, H. H.; Wilson, J. S.; Wilson, P.; Winer, B. L.; Wittich, P.; Wolbers, S.; Wolfe, H.; Wright, T.; Wu, X.; Wu, Z.; Yamamoto, K.; Yamato, D.; Yang, T.; Yang, U. K.; Yang, Y. C.; Yao, W. -M.; Yeh, G. P.; Yi, K.; Yoh, J.; Yorita, K.; Yoshida, T.; Yu, G. B.; Yu, I.; Zanetti, A. M.; Zeng, Y.; Zhou, C.; Zucchelli, S.

    2014-12-31

    We report a measurement of single top quark production in proton-antiproton collisions at a center-of-mass energy of mi>smi>=1.96 mi>TeVmi> using a data set corresponding to 7.5 mi>fbmi>-1 of integrated luminosity collected by the Collider Detector at Fermilab. We select events consistent with the single top quark decay process mi>t>mi>Wmi>b>mi>νmi>b> by requiring the presence of an electron or muon, a large imbalance of transverse momentum indicating the presence of a neutrino, and two or three jets including at least one originating from a bottom quark. An artificial neural network is used to discriminate the signal from backgrounds. We measure a single top quark production cross section of 3.04-0.53+0.57 mi>pb> and set a lower limit on the magnitude of the coupling between the top quark and bottom quark |

  10. Dynamics of miRNA biogenesis and nuclear transport

    Directory of Open Access Journals (Sweden)

    Kotipalli Aneesh

    2016-12-01

    Full Text Available MicroRNAs (miRNAs are short noncoding RNA sequences ~22 nucleotides in length that play an important role in gene regulation-transcription and translation. The processing of these miRNAs takes place in both the nucleus and the cytoplasm while the final maturation occurs in the cytoplasm. Some mature miRNAs with nuclear localisation signals (NLS are transported back to the nucleus and some remain in the cytoplasm. The functional roles of these miRNAs are seen in both the nucleus and the cytoplasm. In the nucleus, miRNAs regulate gene expression by binding to the targeted promoter sequences and affect either the transcriptional gene silencing (TGS or transcriptional gene activation (TGA. In the cytoplasm, targeted mRNAs are translationally repressed or cleaved based on the complementarity between the two sequences at the seed region of miRNA and mRNA. The selective transport of mature miRNAs to the nucleus follows the classical nuclear import mechanism. The classical nuclear import mechanism is a highly regulated process, involving exportins and importins. The nuclear pore complex (NPC regulates all these transport events like a gate keeper. The half-life of miRNAs is rather low, so within a short time miRNAs perform their function. Temporal studies of miRNA biogenesis are, therefore, useful. We have carried out simulation studies for important miRNA biogenesis steps and also classical nuclear import mechanism using ordinary differential equation (ODE solver in the Octave software.

  11. miR-193b Regulates Mcl-1 in Melanoma.

    Science.gov (United States)

    Chen, Jiamin; Zhang, Xiao; Lentz, Cindy; Abi-Daoud, Marie; Paré, Geneviève C; Yang, Xiaolong; Feilotter, Harriet E; Tron, Victor A

    2011-11-01

    MicroRNAs play important roles in gene regulation, and their expression is frequently dysregulated in cancer cells. In a previous study, we reported that miR-193b represses cell proliferation and regulates cyclin D1 in melanoma cells, suggesting that miR-193b could act as a tumor suppressor. Herein, we demonstrate that miR-193b also down-regulates myeloid cell leukemia sequence 1 (Mcl-1) in melanoma cells. MicroRNA microarray profiling revealed that miR-193b is expressed at a significantly lower level in malignant melanoma than in benign nevi. Consistent with this, Mcl-1 is detected at a higher level in malignant melanoma than in benign nevi. In a survey of melanoma samples, the level of Mcl-1 is inversely correlated with the level of miR-193b. Overexpression of miR-193b in melanoma cells represses Mcl-1 expression. Previous studies showed that Mcl-1 knockdown cells are hypersensitive to ABT-737, a small-molecule inhibitor of Bcl-2, Bcl-X(L), and Bcl-w. Similarly, overexpression of miR-193b restores ABT-737 sensitivity to ABT-737-resistant cells. Furthermore, the effect of miR-193b on the expression of Mcl-1 seems to be mediated by direct interaction between miR-193b and seed and seedless pairing sequences in the 3' untranslated region of Mcl-1 mRNA. Thus, this study provides evidence that miR-193b directly regulates Mcl-1 and that down-regulation of miR-193b in vivo could be an early event in melanoma progression. Copyright © 2011 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.

  12. The coordinated roles of miR-26a and miR-30c in regulating TGFβ1-induced epithelial-to-mesenchymal transition in diabetic nephropathy

    DEFF Research Database (Denmark)

    Zheng, Zongji; Guan, Meiping; Jia, Yijie

    2016-01-01

    and miR-30c targeted connective tissue growth factor (CTGF); additionally, Snail family zinc finger 1 (Snail1), a potent epithelial-to-mesenchymal transition (EMT) inducer, was targeted by miR-30c. Overexpression of miR-26a and miR-30c coordinately decreased CTGF protein levels and subsequently...

  13. Miíase na topografia de saco lacrimal

    Directory of Open Access Journals (Sweden)

    Simone Haber Duellberg von Faber Bison

    2016-02-01

    Full Text Available RESUMO A miíase é a infestação dos tecidos humanos por larvas Diptera. O comprometimento ocular é raro. Os autores apresentam um caso de miíase na topografia do saco lacrimal e discutem as modalidades terapêuticas para o tratamento desta doença.

  14. Base Composition Characteristics of Mammalian miRNAs

    Directory of Open Access Journals (Sweden)

    Bin Wang

    2013-01-01

    Full Text Available MicroRNAs (miRNAs are short RNA sequences that repress protein synthesis by either inhibiting the translation of messenger RNA (mRNA or increasing mRNA degradation. Endogenous miRNAs have been found in various organisms, including animals, plants, and viruses. Mammalian miRNAs are evolutionarily conserved, are scattered throughout chromosomes, and play an important role in the immune response and the onset of cancer. For this study, the author explored the base composition characteristics of miRNA genes from the six mammalian species that contain the largest number of known miRNAs. It was found that mammalian miRNAs are evolutionarily conserved and GU-rich. Interestingly, in the miRNA sequences investigated, A residues are clearly the most frequent occupants of positions 2 and 3 of the 5′ end of miRNAs. Unlike G and U residues that may pair with C/U and A/G, respectively, A residues can only pair with U residues of target mRNAs, which may augment the recognition specificity of the 5′ seed region.

  15. Expression of MiR-9 promotes proliferation, migration and ...

    African Journals Online (AJOL)

    Purpose: To investigate the effect of miR-9 on the proliferation, differentiation and migration of human neural stem cells (NSCs). Methods: The expression of miR-9 was investigated by quantitative real-time polymerase chain reaction (RT-PCR). Cell proliferation was assessed by cell counting kit-8 (CCK8) assay, while cell ...

  16. miRNAs in Human Subcutaneous Adipose Tissue

    DEFF Research Database (Denmark)

    Kristensen, Malene M.; Davidsen, Peter K.; Vigelso, Andreas

    2017-01-01

    Objective Obesity is central in the development of insulin resistance. However, the underlying mechanisms still need elucidation. Dysregulated microRNAs (miRNAs; post-transcriptional regulators) in adipose tissue may present an important link. Methods The miRNA expression in subcutaneous adipose ...

  17. Exosomal miRNAs as biomarkers for prostate cancer

    Directory of Open Access Journals (Sweden)

    Nina Pettersen Hessvik

    2013-03-01

    Full Text Available miRNAs are small non-coding RNAs that finely regulate gene expression in cells. Alterations in miRNA expression have been associated with development of cancer, and miRNAs are now being investigated as biomarkers for cancer as well as other diseases. Recently, miRNAs have been found outside cells in body fluids. Extracellular miRNAs exist in different forms - associated with Ago2 proteins, loaded into extracellular vesicles (exosomes, microvesicles or apoptotic bodies or into high density lipoprotein particles. These extracellular miRNAs are probably products of distinct cellular processes, and might therefore play different roles. However, their functions in vivo are currently unknown. In spite of this, they are considered as promising, noninvasive diagnostic and prognostic tools. Prostate cancer is the most common cancer in men in the Western world, but the currently used biomarker (prostate specific antigen has low specificity. Therefore, novel biomarkers are highly needed. In this review we will discuss possible biological functions of extracellular miRNAs, as well as the potential use of miRNAs from extracellular vesicles as biomarkers for prostate cancer.

  18. Exploration of miRNA families for hypotheses generation.

    KAUST Repository

    Kamanu, T.K.; Radovanovic, Aleksandar; Archer, John A.C.; Bajic, Vladimir B.

    2013-01-01

    species. Our results unveil a subclass of miRNAs that may be regulated by genomic imprinting, and also suggest that some miRNA families may be species-specific, as well as chromosome- and/or strand-specific.

  19. Miłosz’s Sojourns in Parallel (Translation Universes

    Directory of Open Access Journals (Sweden)

    Ewa Rajewska

    2012-01-01

    Full Text Available The well known interpretation of Miłosz’s work as an attempt to capture fulness, has been most fully formulated by Jan Błoński’s “Miłosz jak świat” [“Miłosz like a World”]. The author of the article provides a more detailed version of the interpretation, presenting Miłosz’s work as a multiplied universe: in translation and in self-translation. Miłosz’s universe has been multiplied through translation: undertaking translation of so many and so various poets, Miłosz, by extension, translated their poetic worlds. In doing so, he had to go beyond the borders of the world of his own idiom and imagination. Miłosz’s attempts at transgression beyond the borders of his own language and imagination, and into a poetic “parallel universe”, are conducted, according to the present author, in two ways: through similarity and through completion. Miłosz translates works which he which he selected on the principle of an exceptional poetic kinship (for example in his Excerpts from Useful Books. Other translations were an opportunity to test himself on an intriguing poetic material, which he himself would not be willing to create (for example in poetry by Anna Świrszczyńska.

  20. IDENTIFICATION AND CHARACTERIZATION OF NEW miRNAs IN ...

    African Journals Online (AJOL)

    Pathmanaban

    2012-09-20

    Sep 20, 2012 ... simplest and rapid method of identification of miRNAs is relied on in silico analysis. ... (NRs), are available for several plant species and can be used for ... Currently, there are 89 miRNAs deposited under. Gossypium at Plant ...

  1. Meta-analysis using a novel database, miRStress, reveals miRNAs that are frequently associated with the radiation and hypoxia stress-responses.

    Directory of Open Access Journals (Sweden)

    Laura Ann Jacobs

    Full Text Available Organisms are often exposed to environmental pressures that affect homeostasis, so it is important to understand the biological basis of stress-response. Various biological mechanisms have evolved to help cells cope with potentially cytotoxic changes in their environment. miRNAs are small non-coding RNAs which are able to regulate mRNA stability. It has been suggested that miRNAs may tip the balance between continued cytorepair and induction of apoptosis in response to stress. There is a wealth of data in the literature showing the effect of environmental stress on miRNAs, but it is scattered in a large number of disparate publications. Meta-analyses of this data would produce added insight into the molecular mechanisms of stress-response. To facilitate this we created and manually curated the miRStress database, which describes the changes in miRNA levels following an array of stress types in eukaryotic cells. Here we describe this database and validate the miRStress tool for analysing miRNAs that are regulated by stress. To validate the database we performed a cross-species analysis to identify miRNAs that respond to radiation. The analysis tool confirms miR-21 and miR-34a as frequently deregulated in response to radiation, but also identifies novel candidates as potentially important players in this stress response, including miR-15b, miR-19b, and miR-106a. Similarly, we used the miRStress tool to analyse hypoxia-responsive miRNAs. The most frequently deregulated miRNAs were miR-210 and miR-21, as expected. Several other miRNAs were also found to be associated with hypoxia, including miR-181b, miR-26a/b, miR-106a, miR-213 and miR-192. Therefore the miRStress tool has identified miRNAs with hitherto unknown or under-appreciated roles in the response to specific stress types. The miRStress tool, which can be used to uncover new insight into the biological roles of miRNAs, and also has the potential to unearth potential biomarkers for

  2. Post-transcriptional generation of miRNA variants by multiple nucleotidyl transferases contributes to miRNA transcriptome complexity.

    Science.gov (United States)

    Wyman, Stacia K; Knouf, Emily C; Parkin, Rachael K; Fritz, Brian R; Lin, Daniel W; Dennis, Lucas M; Krouse, Michael A; Webster, Philippa J; Tewari, Muneesh

    2011-09-01

    Modification of microRNA sequences by the 3' addition of nucleotides to generate so-called "isomiRs" adds to the complexity of miRNA function, with recent reports showing that 3' modifications can influence miRNA stability and efficiency of target repression. Here, we show that the 3' modification of miRNAs is a physiological and common post-transcriptional event that shows selectivity for specific miRNAs and is observed across species ranging from C. elegans to human. The modifications result predominantly from adenylation and uridylation and are seen across tissue types, disease states, and developmental stages. To quantitatively profile 3' nucleotide additions, we developed and validated a novel assay based on NanoString Technologies' nCounter platform. For certain miRNAs, the frequency of modification was altered by processes such as cell differentiation, indicating that 3' modification is a biologically regulated process. To investigate the mechanism of 3' nucleotide additions, we used RNA interference to screen a panel of eight candidate miRNA nucleotidyl transferases for 3' miRNA modification activity in human cells. Multiple enzymes, including MTPAP, PAPD4, PAPD5, ZCCHC6, ZCCHC11, and TUT1, were found to govern 3' nucleotide addition to miRNAs in a miRNA-specific manner. Three of these enzymes-MTPAP, ZCCHC6, and TUT1-have not previously been known to modify miRNAs. Collectively, our results indicate that 3' modification observed in next-generation small RNA sequencing data is a biologically relevant process, and identify enzymatic mechanisms that may lead to new approaches for modulating miRNA activity in vivo.

  3. Repertoire of bovine miRNA and miRNA-like small regulatory RNAs expressed upon viral infection.

    Directory of Open Access Journals (Sweden)

    Evgeny A Glazov

    Full Text Available MicroRNA (miRNA and other types of small regulatory RNAs play a crucial role in the regulation of gene expression in eukaryotes. Several distinct classes of small regulatory RNAs have been discovered in recent years. To extend the repertoire of small RNAs characterized in mammals and to examine relationship between host miRNA expression and viral infection we used Illumina's ultrahigh throughput sequencing approach. We sequenced three small RNA libraries prepared from cell line derived from the adult bovine kidney under normal conditions and upon infection of the cell line with Bovine herpesvirus 1. We used a bioinformatics approach to distinguish authentic mature miRNA sequences from other classes of small RNAs and short RNA fragments represented in the sequencing data. Using this approach we detected 219 out of 356 known bovine miRNAs and 115 respective miRNA* sequences. In addition we identified five new bovine orthologs of known mammalian miRNAs and discovered 268 new cow miRNAs many of which are not identifiable in other mammalian genomes and thus might be specific to the ruminant lineage. In addition we found seven new bovine mirtron candidates. We also discovered 10 small nucleolar RNA (snoRNA loci that give rise to small RNA with possible miRNA-like function. Results presented in this study extend our knowledge of the biology and evolution of small regulatory RNAs in mammals and illuminate mechanisms of small RNA biogenesis and function. New miRNA sequences and the original sequencing data have been submitted to miRNA repository (miRBase and NCBI GEO archive respectively. We envisage that these resources will facilitate functional annotation of the bovine genome and promote further functional and comparative genomics studies of small regulatory RNA in mammals.

  4. A values-based Motivational Interviewing (MI) intervention for pediatric obesity: study design and methods for MI Values.

    Science.gov (United States)

    Bean, Melanie K; Mazzeo, Suzanne E; Stern, Marilyn; Bowen, Deborah; Ingersoll, Karen

    2011-09-01

    To reduce pediatric obesity in clinical settings, multidisciplinary behaviorally-based treatment programs are recommended. High attrition and poor compliance are two difficulties frequently encountered in such programs. A brief, empathic and directive clinical intervention, Motivational Interviewing (MI), might help address these motivational and behavioral issues, ultimately resulting in more positive health outcomes. The efficacy of MI as an adjunct in the treatment of pediatric obesity remains relatively understudied. MI Values was developed to implement within an existing multidisciplinary treatment program for obese, ethnically diverse adolescents, the T.E.E.N.S. Program (Teaching, Encouragement, Exercise, Nutrition, Support). T.E.E.N.S. participants who consent to MI Values are randomized to either MI or an education control condition. At weeks 1 and 10 of T.E.E.N.S. participation, the subset of participants assigned to the MI condition engages in individual MI sessions and control participants view health education videos. All MI sessions are audiotaped and coded to monitor treatment fidelity, which has been satisfactory thus far. Participants complete comprehensive assessments at baseline, 3- and 6-month follow-ups. We hypothesize that MI participants will demonstrate greater reductions in Body Mass Index (BMI) percentile, improved diet and physical activity behaviors, better compliance with T.E.E.N.S., and lower attrition than participants in the control group. We present study design and methods for MI Values as well as data on feasibility of recruitment methods and treatment integrity. At study completion, findings will contribute to the emerging literature examining the efficacy of MI in the treatment of pediatric obesity. Copyright © 2011 Elsevier Inc. All rights reserved.

  5. Regulation of turkey myogenic satellite cell migration by MicroRNAs miR-128 and miR-24.

    Science.gov (United States)

    Velleman, S G; Harding, R L

    2017-06-01

    Myogenic satellite cells are an adult stem cell responsible for all post-hatch muscle growth in poultry. As a stem cell population, satellite cells are highly heterogeneous, but the origin of this heterogeneity remains unclear. Heterogeneity is, in part, regulated by gene expression. One method of endogenous gene regulation that may contribute to heterogeneity is microRNAs (miRNAs). Two miRNAs previously shown to regulate poultry myogenic satellite cell proliferation and differentiation, miR-128 and miR-24, were studied to determine if they also affected satellite cell migration. Satellite cell migration is an essential step for both proliferation and differentiation. During proliferation, satellite cells will migrate and align to form new myofibers or donate their nuclei to existing myofibers leading to muscle fiber hypertrophy or regeneration. Transient transfection of miRNA specific mimics to each miRNA reduced migration of satellite cells following a cell culture scratch at 72 h of proliferation when the cultures were 90 to 100% confluent. However, only the migration in cells transfected with miR-24 mimics at 24 and 30 h following the scratch was significantly reduced (P ≤ 0.05) to around 70% of the distance migrated by controls. Alternately, transfection with inhibitors specific to miR-128 or miR-24 significantly (P ≤ 0.05) increased migration between 147 and 252% compared to their controls between 24 and 48 h following the scratch. These data demonstrate that miR-128 and miR-24 play a role in myogenic satellite cell migration, which will impact muscle development and growth. © 2016 Poultry Science Association Inc.

  6. Heterogeneity of miRNA expression in localized prostate cancer with clinicopathological correlations

    DEFF Research Database (Denmark)

    Zedan, Ahmed Hussein; Blavnsfeldt, Søren Garm; Hansen, Torben Frøstrup

    2017-01-01

    ).RESULTS: Four miRNAs (miRNA-21, miRNA-34a, miRNA-125, and miRNA-126) were significantly upregulated in PCa compared to benign prostatic hyperplasia (BPH), and except for miRNA-21 these miRNAs documented a positive correlation between the expression level in PCa cores and their matched BPH cores, (r > 0......-free survival (p = 0.016).CONCLUSION: The present study documents significant upregulation of the expression of miRNA-21, miRNA-34a, miRNA-125, and miRNA-126 in PCa compared to BPH and suggests a possible prognostic value associated with the expression of miRNA-143. The results, however, document intra...

  7. Heterogeneity of miRNA expression in localized prostate cancer with clinicopathological correlations

    DEFF Research Database (Denmark)

    Zedan, Ahmed Hussein; Blavnsfeldt, Søren Garm; Hansen, Torben Frøstrup

    2017-01-01

    ). RESULTS: Four miRNAs (miRNA-21, miRNA-34a, miRNA-125, and miRNA-126) were significantly upregulated in PCa compared to benign prostatic hyperplasia (BPH), and except for miRNA-21 these miRNAs documented a positive correlation between the expression level in PCa cores and their matched BPH cores, (r > 0......-free survival (p = 0.016). CONCLUSION: The present study documents significant upregulation of the expression of miRNA-21, miRNA-34a, miRNA-125, and miRNA-126 in PCa compared to BPH and suggests a possible prognostic value associated with the expression of miRNA-143. The results, however, document intra...

  8. 78 FR 65380 - Notice of Inventory Completion: University of Michigan, Ann Arbor, MI

    Science.gov (United States)

    2013-10-31

    ... the University of Michigan, Ann Arbor, MI. The human remains were removed from Alpena, Isabella, Grand... removed from the Devil River Mound site (20AL1) in Alpena County, MI. A resident of Ossineke, MI...

  9. Year-class formation of upper St. Lawrence River northern pike

    Science.gov (United States)

    Smith, B.M.; Farrell, J.M.; Underwood, H.B.; Smith, S.J.

    2007-01-01

    Variables associated with year-class formation in upper St. Lawrence River northern pike Esox lucius were examined to explore population trends. A partial least-squares (PLS) regression model (PLS 1) was used to relate a year-class strength index (YCSI; 1974-1997) to explanatory variables associated with spawning and nursery areas (seasonal water level and temperature and their variability, number of ice days, and last day of ice presence). A second model (PLS 2) incorporated four additional ecological variables: potential predators (abundance of double-crested cormorants Phalacrocorax auritus and yellow perch Perca flavescens), female northern pike biomass (as a measure of stock-recruitment effects), and total phosphorus (productivity). Trends in adult northern pike catch revealed a decline (1981-2005), and year-class strength was positively related to catch per unit effort (CPUE; R2 = 0.58). The YCSI exceeded the 23-year mean in only 2 of the last 10 years. Cyclic patterns in the YCSI time series (along with strong year-classes every 4-6 years) were apparent, as was a dampening effect of amplitude beginning around 1990. The PLS 1 model explained over 50% of variation in both explanatory variables and the dependent variable, YCSI first-order moving-average residuals. Variables retained (N = 10; Wold's statistic ??? 0.8) included negative YCSI associations with high summer water levels, high variability in spring and fall water levels, and variability in fall water temperature. The YCSI exhibited positive associations with high spring, summer, and fall water temperature, variability in spring temperature, and high winter and spring water level. The PLS 2 model led to positive YCSI associations with phosphorus and yellow perch CPUE and a negative correlation with double-crested cormorant abundance. Environmental variables (water level and temperature) are hypothesized to regulate northern pike YCSI cycles, and dampening in YCSI magnitude may be related to a

  10. Fate of oil-SPM aggregates in the St-Lawrence estuary

    International Nuclear Information System (INIS)

    Khelifa, A.; Brown, C.

    2009-01-01

    The formation of aggregates of oil-suspended particulate matter (SPM) has been studied extensively over the last 4 decades. This paper presented the results of a full scale numerical study that examined the fate of oil-SPM aggregates (OSAs) formed in the St-Lawrence estuary in both shallow and deep water. OSAs form from the natural heteroaggregation between suspended oil droplets and SPM following an oil spill in the aquatic environment. Until recently, it was believed that this natural process helps clean oiled shorelines by dispersing spilled oil in coastal waters rich in SPM. However, new studies have shown that the density of OSAs may exceed the density of the seawater and their formation may result in the transfer of considerable amounts of oil from the water column to the seafloor. This study was conducted using real hydrodynamic data, recent laboratory data related to the size, density and oil content of OSAs and the commercial CHEMMAP software. Simulations were run for 5 days for each scenario. The concentrations of OSAs in the water column and on the seafloor were tracked in space and time. According to the results, the size and density of OSAs have a strong influence on the fate of OSAs, as do water depth and current density. OSAs of 200 μm in size and higher settled within half a day in shallow water zones, and within 5 days in deep water zones. The resulting oil concentration in bottom sediment showed strong increase with the size of OSA in shallow water, but small increase in deep water. Sedimentation of OSAs with an average size of 200 μm is affected by the effective density. In general, horizontal mixing had a much smaller effect on the sedimentation of OSAs than those obtained with their size and effective density. It was concluded that ideally, the fate of OSAs should be addressed using a dynamic model that relates the size of OSAs to local intensity of turbulence. 20 refs., 1 tab., 10 figs.

  11. Historic Context and Building Assessments for the Lawrence Livermore National Laboratory Built Environment

    Energy Technology Data Exchange (ETDEWEB)

    Ullrich, R. A. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Sullivan, M. A. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2007-09-14

    This document was prepared to support u.s. Department of Energy / National Nuclear Security Agency (DOE/NNSA) compliance with Sections 106 and 110 of the National Historic Preservation Act (NHPA). Lawrence Livermore National Laboratory (LLNL) is a DOE/NNSA laboratory and is engaged in determining the historic status of its properties at both its main site in Livermore, California, and Site 300, its test site located eleven miles from the main site. LLNL contracted with the authors via Sandia National Laboratories (SNL) to prepare a historic context statement for properties at both sites and to provide assessments of those properties of potential historic interest. The report contains an extensive historic context statement and the assessments of individual properties and groups of properties determined, via criteria established in the context statement, to be of potential interest. The historic context statement addresses the four contexts within which LLNL falls: Local History, World War II History (WWII), Cold War History, and Post-Cold War History. Appropriate historic preservation themes relevant to LLNL's history are delineated within each context. In addition, thresholds are identified for historic significance within each of the contexts based on the explication and understanding of the Secretary of the Interior's Guidelines for determining eligibility for the National Register of Historic Places. The report identifies specific research areas and events in LLNL's history that are of interest and the portions of the built environment in which they occurred. Based on that discussion, properties of potential interest are identified and assessments of them are provided. Twenty individual buildings and three areas of potential historic interest were assessed. The final recommendation is that, of these, LLNL has five individual historic buildings, two sets of historic objects, and two historic districts eligible for the National Register. All are

  12. Lawrence Livermore National Laboratory Workshop Characterization of Pathogenicity, Virulence and Host-Pathogen Interactions

    Energy Technology Data Exchange (ETDEWEB)

    Krishnan, A

    2006-08-30

    The threats of bio-terrorism and newly emerging infectious diseases pose serious challenges to the national security infrastructure. Rapid detection and diagnosis of infectious disease in human populations, as well as characterizing pathogen biology, are critical for reducing the morbidity and mortality associated with such threats. One of the key challenges in managing an infectious disease outbreak, whether through natural causes or acts of overt terrorism, is detection early enough to initiate effective countermeasures. Much recent attention has been directed towards the utility of biomarkers or molecular signatures that result from the interaction of the pathogen with the host for improving our ability to diagnose and mitigate the impact of a developing infection during the time window when effective countermeasures can be instituted. Host responses may provide early signals in blood even from localized infections. Multiple innate and adaptive immune molecules, in combination with other biochemical markers, may provide disease-specific information and new targets for countermeasures. The presence of pathogen specific markers and an understanding of the molecular capabilities and adaptations of the pathogen when it interacts with its host may likewise assist in early detection and provide opportunities for targeting countermeasures. An important question that needs to be addressed is whether these molecular-based approaches will prove useful for early diagnosis, complement current methods of direct agent detection, and aid development and use of countermeasures. Lawrence Livermore National Laboratory (LLNL) will host a workshop to explore the utility of host- and pathogen-based molecular diagnostics, prioritize key research issues, and determine the critical steps needed to transition host-pathogen research to tools that can be applied towards a more effective national bio-defense strategy. The workshop will bring together leading researchers/scientists in the

  13. An Overview of the Target Fabrication Operations at Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    Hibbard, R L; Bono, M J

    2005-01-01

    The Target Engineering team at Lawrence Livermore National Laboratory (LLNL) builds precision laser targets for the National Ignition Facility (NIF) and the Omega Laser in Rochester, NY, and other experimental facilities. The physics requirements demand precision in these targets, which creates a constant need for innovative manufacturing processes. As experimental diagnostics improve, there is greater demand for precision in fabrication, assembly, metrology, and documentation of as-built targets. The team specializes in meso-scale fabrication with core competencies in diamond turning, assembly, and metrology. Figure 1 shows a typical diamond turning center. The team builds over 200 laser targets per year in batches of five to fifteen targets. Thus, all are small-lot custom builds, and most are novel designs requiring engineering and process development. Component materials are metals, polymers and low density aerogel foams. Custom fixturing is used to locate parts on the Diamond Turning Machines (DTM) and assembly stations. This ensures parts can be repeatably located during manufacturing operations. Most target builds involve a series of fabricating one surface with features and then relocating the components on another fixture to finish the opposite side of the component. These components are then assembled to complete multiple-component targets. These targets are typically built one at a time. Cost and efficiency are issues with production of targets, and the team is developing batch processing techniques to meet precision target specifications and cost goals. Three example target builds will highlight some of the fabrication and material issues faced at LLNL. A low temperature Rayleigh Taylor target shows how multiple precision targets can be fabricated out of a single large disk. The ignition double shell targets highlight the required manufacturing complexity. A low density aerogel target highlights some material handling and assembly issues. The metrology

  14. The copper-pumped dye laser system at Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    Hackel, R.P.; Warner, B.E.

    1993-01-01

    The Lawrence Livermore National Laboratory's (LLNL) Atomic Vapor Laser Isotope Separation (AVLIS) Program has developed a high-average-power, pulsed, tunable, visible laser system. Testing of this hardware is in progress at industrial scale. The LLNL copper-dye laser system is prototypical of a basic module of a uranium-AVLIS plant. The laser demonstration facility (LDF) system consists of copper vapor lasers arranged in oscillator-amplifier chains providing optical pump power to dye-laser master-oscillator-power-amplifier chains. This system is capable of thousands of watts (average) tunable between 550 and 650 mm. The copper laser system at LLNL consists of 12 chains operating continuously. The copper lasers operate at nominally 4.4 kHz, with 50 ns pulse widths and produce 20 W at near the diffraction limit from oscillators and >250 W from each amplifier. Chains consist of an oscillator and three amplifiers and produce >750 W average, with availabilities >95% (i.e., >8,300 h/y). The total copper laser system power averages ∼9,000 W and has operated at over 10,000 W for extended intervals. The 12 copper laser beams are multiplexed and delivered to the dye laser system where they pump multiple dye laser chains. Each dye chain consists of a master oscillator and three or four power amplifiers. The master oscillator operates at nominally 100 mW with a 50 MHz single mode bandwidth. Amplifiers are designed to efficiently amplify the dye beam with low ASE content and high optical quality. Sustained dye chain powers are up to 1,400 W with dye conversion efficiency >50%, ASE content <5%, and wavefront quality correctable to <λ/10 RMS, using deformable mirrors. Since the timing of the copper laser chains can be offset, the dye laser system is capable of repetition rates which are multiples of 4.4 kHz, up to 26 kHz, limited by the dye pumping system. Development of plant-scale copper and dye laser hardware is progressing in off-line facilities

  15. Expression of miR-15a, miR-145, and miR-182 in granulosa-lutein cells, follicular fluid, and serum of women with polycystic ovary syndrome (PCOS).

    Science.gov (United States)

    Naji, Mohammad; Nekoonam, Saeid; Aleyasin, Ashraf; Arefian, Ehsan; Mahdian, Reza; Azizi, Elham; Shabani Nashtaei, Maryam; Amidi, Fardin

    2018-01-01

    Polycystic ovary syndrome (PCOS) is one of the most common endocrinopathies that affects women in reproductive age. MicroRNAs (miRNAs) play crucial roles in normal function of female reproductive system and folliculogenesis. Deregulated expression of miRNAs in PCOS condition may be significantly implicated in the pathogenesis of PCOS. We determined relative expression of miR-15a, miR-145, and miR-182 in granulosa-lutein cells (GLCs), follicular fluid (FF), and serum of PCOS patients. Human subjects were divided into PCOS (n = 20) and control (n = 21) groups. GLCs, FF, and serum were isolated and stored. RNA isolation was performed and cDNA was reversely transcribed using specific stem-loop RT primers. Relative expression of miRNAs was calculated after normalization against U6 expression. Correlation of miRNAs' expression level with basic clinical features and predictive value of miRNAs in FF and serum were appraised. Relative expression of miR-145 and miR-182 in GLCs was significantly decreased in PCOS, but miR-182 in FF of PCOS patients revealed up-regulated levels. Significant correlations between level of miRNAs in FF and serum and hormonal profile of subjects were observed. MiR-182 in FF showed a significant predictive value with AUC of 0.73, 76.4% sensitivity, and 70.5% specificity which was improved after combination of miR-182 and miR-145. A significant dysregulation of miR-145 and miR-182 in GLCs of PCOS may indicate their involvement in pathogenesis of PCOS. Differential up-regulation of miR-182 in FF of PCOS patients with its promising predictive values for discrimination of PCOS reinforced the importance of studying miRNAs' profile in FF.

  16. miRNAs in Normal and Malignant Hematopoiesis

    Directory of Open Access Journals (Sweden)

    Ryutaro Kotaki

    2017-07-01

    Full Text Available Lineage specification is primarily regulated at the transcriptional level and lineage-specific transcription factors determine cell fates. MicroRNAs (miRNAs are 18–24 nucleotide-long non-coding RNAs that post-transcriptionally decrease the translation of target mRNAs and are essential for many cellular functions. miRNAs also regulate lineage specification during hematopoiesis. This review highlights the roles of miRNAs in B-cell development and malignancies, and discusses how miRNA expression profiles correlate with disease prognoses and phenotypes. We also discuss the potential for miRNAs as therapeutic targets and diagnostic tools for B-cell malignancies.

  17. miRNAs as therapeutic targets in ischemic heart disease.

    Science.gov (United States)

    Frost, Robert J A; van Rooij, Eva

    2010-06-01

    Ischemic heart disease is a form of congestive heart failure that is caused by insufficient blood supply to the heart, resulting in a loss of viable tissue. In response to the injury, the non-ischemic myocardium displays signs of secondary remodeling, like interstitial fibrosis and hypertrophy of cardiac myocytes. This remodeling process further deteriorates pump function and increases susceptibility to arrhythmias. MicroRNAs (miRNAs) are small, non-coding RNAs that regulate gene expression in a sequence-dependent manner. Recently, several groups identified miRNAs as crucial gene regulators in response to myocardial infarction (MI) and during post-MI remodeling. In this review, we discuss how modulation of these miRNAs represents a promising new therapeutic strategy to improve the clinical outcome in ischemic heart disease.

  18. Towards an understanding of miRNA regulation

    DEFF Research Database (Denmark)

    Jensen, Trine Ilsø

    miRNAs are well-known regulators of gene expression. They function post-transcriptionally by binding to complementary sites within the 3´UTR of target mRNAs, which mediates translational repression and destabilization. However, miRNA expression itself is also subjected to regulation. Here, we...... report a new method to investigate and potentially characterize the pri-miRNA transcript. Overexpression of a transdominant Drosha mutant, which is unable to cleave its substrate, enables stabilization of the pri-miRNA transcript. Drosha mutant immunoprecipitation from the nuclear compartment...... is performed followed by high-throughput sequencing (nuclear Drosha Mt2 RIPseq). This method allows for the detection of global pri-miRNA signature and also provides a method to potentially identify new Drosha substrates. Furthermore, data on the identification of a novel endogenous circular RNA sponge (ciRS-7...

  19. Growth inhibitory effects of miR-221 and miR-222 in non-small cell lung cancer cells

    International Nuclear Information System (INIS)

    Yamashita, Ryo; Sato, Mitsuo; Kakumu, Tomohiko; Hase, Tetsunari; Yogo, Naoyuki; Maruyama, Eiichi; Sekido, Yoshitaka; Kondo, Masashi; Hasegawa, Yoshinori

    2015-01-01

    Both pro- and anti-oncogenic roles of miR-221 and miR-222 microRNAs are reported in several types of human cancers. A previous study suggested their oncogenic role in invasiveness in lung cancer, albeit only one cell line (H460) was used. To further evaluate involvement of miR-221 and miR-222 in lung cancer, we investigated the effects of miR-221 and miR-222 overexpression on six lung cancer cell lines, including H460, as well as one immortalized normal human bronchial epithelial cell line, HBEC4. miR-221 and miR-222 induced epithelial-to-mesenchymal transition (EMT)-like changes in a minority of HBEC4 cells but, unexpectedly, both the microRNAs rather suppressed their invasiveness. Consistent with the prior report, miR-221 and miR-222 promoted growth in H460; however, miR-221 suppressed growth in four other cell lines with no effects in one, and miR-222 suppressed growth in three cell lines but promoted growth in two. These are the first results to show tumor-suppressive effects of miR-221 and miR-222 in lung cancer cells, and we focused on clarifying the mechanisms. Cell cycle and apoptosis analyses revealed that growth suppression by miR-221 and miR-222 occurred through intra-S-phase arrest and/or apoptosis. Finally, lung cancer cell lines transfected with miR-221 or miR-222 became more sensitive to the S-phase targeting drugs, possibly due to an increased S-phase population. In conclusion, our data are the first to show tumor-suppressive effects of miR-221 and miR-222 on lung cancer, warranting testing their potential as therapeutics for the disease

  20. Role for DNA methylation in the regulation of miR-200c and miR-141 expression in normal and cancer cells

    Energy Technology Data Exchange (ETDEWEB)

    Vrba, Lukas; Jensen, Taylor J.; Garbe, James C.; Heimark, Ronald L.; Cress, Anne E.; Dickinson, Sally; Stampfer, Martha R.; Futscher, Bernard W.

    2009-12-23

    BACKGROUND: The microRNA-200 family participates in the maintenance of an epithelial phenotype and loss of its expression can result in epithelial to mesenchymal transition (EMT). Furthermore, the loss of expression of miR-200 family members is linked to an aggressive cancer phenotype. Regulation of the miR-200 family expression in normal and cancer cells is not fully understood. METHODOLOGY/ PRINCIPAL FINDINGS: Epigenetic mechanisms participate in the control of miR-200c and miR-141 expression in both normal and cancer cells. A CpG island near the predicted mir-200c/mir-141 transcription start site shows a striking correlation between miR-200c and miR-141 expression and DNA methylation in both normal and cancer cells, as determined by MassARRAY technology. The CpG island is unmethylated in human miR-200/miR-141 expressing epithelial cells and in miR-200c/miR-141 positive tumor cells. The CpG island is heavily methylated in human miR-200c/miR-141 negative fibroblasts and miR-200c/miR-141 negative tumor cells. Mouse cells show a similar inverse correlation between DNA methylation and miR-200c expression. Enrichment of permissive histone modifications, H3 acetylation and H3K4 trimethylation, is seen in normal miR-200c/miR-141-positive epithelial cells, as determined by chromatin immunoprecipitation coupled to real-time PCR. In contrast, repressive H3K9 dimethylation marks are present in normal miR-200c/miR-141-negative fibroblasts and miR-200c/miR-141 negative cancer cells and the permissive histone modifications are absent. The epigenetic modifier drug, 5-aza-2'-deoxycytidine, reactivates miR-200c/miR-141 expression showing that epigenetic mechanisms play a functional role in their transcriptional control. CONCLUSIONS/ SIGNIFICANCE: We report that DNA methylation plays a role in the normal cell type-specific expression of miR-200c and miR-141 and this role appears evolutionarily conserved, since similar results were obtained in mouse. Aberrant DNA methylation

  1. miRNA-target chimeras reveal miRNA 3'-end pairing as a major determinant of Argonaute target specificity

    DEFF Research Database (Denmark)

    Moore, Michael J; Scheel, Troels K H; Luna, Joseph M

    2015-01-01

    microRNAs (miRNAs) act as sequence-specific guides for Argonaute (AGO) proteins, which mediate posttranscriptional silencing of target messenger RNAs. Despite their importance in many biological processes, rules governing AGO-miRNA targeting are only partially understood. Here we report a modifie...

  2. Diagnostic accuracy of serum miR-122 and miR-199a in women with endometriosis.

    Science.gov (United States)

    Maged, Ahmed M; Deeb, Wesam S; El Amir, Azza; Zaki, Sherif S; El Sawah, Heba; Al Mohamady, Maged; Metwally, Ahmed A; Katta, Maha A

    2018-04-01

    To evaluate the value of serum microRNA-122 (miR-122) and miR-199a as reliable noninvasive biomarkers in the diagnosis of endometriosis. During 2015-2016, at a teaching hospital in Egypt, a prospective cohort study was conducted on 45 women with pelvic endometriosis and 35 women who underwent laparoscopy for pelvic pain but were not diagnosed with endometriosis. Blood and peritoneal fluid (PF) samples were collected; interleukin-6 (IL-6) was detected by enzyme-linked immunosorbent assay and miR-122 and miR-199a expression was measured by quantitative real-time polymerase chain reaction. The serum and PF levels of IL-6, miR-122, and miR-199a were significantly higher in women with endometriosis than in controls (Pendometriosis. Serum miR-122 and miR-199a were significantly increased in endometriosis, indicating that these microRNAs might serve as biomarkers for the diagnosis of endometriosis. © 2017 International Federation of Gynecology and Obstetrics.

  3. Data of expression status of miR- 29a and its putative target mitochondrial apoptosis regulatory gene DRP1 upon miR-15a and miR-214 inhibition

    Directory of Open Access Journals (Sweden)

    Muhammad Ishtiaq Jan

    2018-02-01

    Full Text Available Data is about the mitochondrial apoptosis regulatory framework genes PUMA, DRP1 (apoptotic, and ARC (anti-apoptotic analysis after the employment of their controlling miRNAs inhibitors. The data represents putative conserved targeting of seed regions of miR-15a, miR-29a, and miR-214 with respective target genes PUMA, DRP1, and ARC. Data is of cross interference in expression levels of one miRNA family, miR-29a and its putative target DRP1 upon the inhibitory treatment of other miRNAs 15a and 214. Keywords: DRP1, miR-15a, Apoptosis, miRNAs inhibition

  4. Common miR-590 Variant rs6971711 Present Only in African Americans Reduces miR-590 Biogenesis.

    Directory of Open Access Journals (Sweden)

    Xiaoping Lin

    Full Text Available MicroRNAs (miRNAs are recognized as important regulators of cardiac development, hypertrophy and fibrosis. Recent studies have demonstrated that genetic variations which cause alterations in miRNA:target interactions can lead to disease. We hypothesized that genetic variations in miRNAs that regulate cardiac hypertrophy/fibrosis might be involved in generation of the cardiac phenotype in patients diagnosed with hypertrophic cardiomyopathy (HCM. To investigate this question, we Sanger sequenced 18 miRNA genes previously implicated in myocyte hypertrophy/fibrosis and apoptosis, using genomic DNA isolated from the leukocytes of 199 HCM patients. We identified a single nucleotide polymorphism (rs6971711, C57T SNP at the 17th position of mature miR-590-3p (= 57th position of pre-miR-590 that is common in individuals of African ancestry. SNP frequency was higher in African American HCM patients (n = 55 than ethnically-matched controls (n = 100, but the difference was not statistically significant (8.2% vs. 6.5%; p = 0.5. Using a cell culture system, we discovered that presence of this SNP resulted in markedly lower levels of mature miR-590-5p (39 ± 16%, p<0.003 and miR-590-3p (20 ± 2%, p<0.003, when compared with wild-type (WT miR-590, without affecting levels of pri-miR-590 and pre-miR-590. Consistent with this finding, the SNP resulted in reduced target suppression when compared to WT miR-590 (71% suppression by WT vs 60% suppression by SNP, p<0.03. Since miR-590 can regulate TGF-β, Activin A and Akt signaling, SNP-induced reduction in miR-590 biogenesis could influence cardiac phenotype by de-repression of these signaling pathways. Since the SNP is only present in African Americans, population studies in this patient population would be valuable to investigate effects of this SNP on myocyte function and cardiac physiology.

  5. Impact of Type 2 Myocardial Infarction (MI) on Hospital-Level MI Outcomes: Implications for Quality and Public Reporting.

    Science.gov (United States)

    Arora, Sameer; Strassle, Paula D; Qamar, Arman; Wheeler, Evan N; Levine, Alexandra L; Misenheimer, Jacob A; Cavender, Matthew A; Stouffer, George A; Kaul, Prashant

    2018-03-26

    The International Classification of Diseases (ICD) coding system does not recognize type 2 myocardial infarction (MI) as a separate entity; therefore, patients with type 2 MI continue to be categorized under the general umbrella of non-ST-segment-elevation myocardial infarction (NSTEMI). We aim to evaluate the impact of type 2 MI on hospital-level NSTEMI metrics and discuss the implications for quality and public reporting. We conducted a single-center retrospective analysis of 1318 patients discharged with a diagnosis of NSTEMI between July 2013 and October 2014. The Third Universal Definition was used to define type 1 and type 2 MI. Weighted Kaplan-Meier curves were used to analyze risk of mortality and readmission. Overall, 1039 patients met NSTEMI criteria per the Third Universal Definition; of those, 264 (25.4%) had type 2 MI. Patients with type 2 MI were older, were more likely to have chronic kidney disease, and had lower peak troponin levels. Compared with type 1 MI patients, those with type 2 MI had higher inpatient mortality (17.4% versus 4.7%, P <0.0001) and were more likely to die from noncardiovascular causes (71.7% versus 25.0%, P <0.0001). Despite weighting for patient characteristics and discharge medications, patients with type 2 MI had higher mortality at both 30 days (risk ratio: 3.63; 95% confidence interval, 1.67-7.88) and 1 year (risk ratio: 1.98; 95% confidence interval, 1.44-2.73) after discharge. Type 2 MI was also associated with a lower 30-day cardiovascular-related readmission (risk ratio: 0.49; 95% confidence interval, 0.12-2.06). NSTEMI metrics are significantly affected by type 2 MI patients. Type 2 MI patients have distinct etiologies, are managed differently, and have higher mortality compared with patients with type 1 MI. Moving forward, it may be appropriate to exclude type 2 MI data from NSTEMI quality metrics. © 2018 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley.

  6. Spatial trends of co-planar contaminants in lobster digestive gland from the Gulf of St. Lawrence

    Energy Technology Data Exchange (ETDEWEB)

    Fairchild, W.L.; Arsenault, J.T.; Comeau, M. [Department of Fisheries and Oceans, Moncton, NB (Canada)

    1997-05-01

    A study on spatial trends of contaminants in Canadian marine biota was conducted between 1993 and 1995. Lobster digestive glands were sampled from eight locations on the Gulf of St. Lawrence near New Brunswick and Nova Scotia. A total of 15 gland samples were analysed for dioxin, furan and co-planar PCB congeners. The five highest concentrations measured (5.8-12 pg/g wet-weight) were from samples taken from within the barrier island of Miramichi Bay in New Brunswick. This is an area of municipal and industrial development where contamination levels in marine biota is a continuing local concern.

  7. Low-level waste certification plan for the Lawrence Berkeley Laboratory Hazardous Waste Handling Facility. Revision 1

    International Nuclear Information System (INIS)

    1995-01-01

    The purpose of this plan is to describe the organization and methodology for the certification of low-level radioactive waste (LLW) handled in the Hazardous Waste Handling Facility (HWHF) at Lawrence Berkeley Laboratory (LBL). This plan is composed to meet the requirements found in the Westinghouse Hanford Company (WHC) Solid Waste Acceptance Criteria (WAC) and follows the suggested outline provided by WHC in the letter of April 26, 1990, to Dr. R.H. Thomas, Occupational Health Division, LBL. LLW is to be transferred to the WHC Hanford Site Central Waste Complex and Burial Grounds in Hanford, Washington

  8. Environmental assessment for construction and operation of a Human Genome Laboratory at Lawrence Berkeley Laboratory, Berkeley, California

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1994-12-01

    Lawrence Berkeley Laboratory (LBL) proposes to construct and operate a new laboratory for consolidation of current and future activities of the Human Genome Center (HGC). This document addresses the potential direct, indirect, and cumulative environmental and human-health effects from the proposed facility construction and operation. This document was prepared in accordance the National Environmental Policy Act of 1969 (United States Codes 42 USC 4321-4347) (NEPA) and the US Department of Energy`s (DOE) Final Rule for NEPA Implementing Procedures [Code of Federal Regulations 10CFR 1021].

  9. Low-level waste certification plan for the Lawrence Berkeley Laboratory Hazardous Waste Handling Facility. Revision 1

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-01-10

    The purpose of this plan is to describe the organization and methodology for the certification of low-level radioactive waste (LLW) handled in the Hazardous Waste Handling Facility (HWHF) at Lawrence Berkeley Laboratory (LBL). This plan is composed to meet the requirements found in the Westinghouse Hanford Company (WHC) Solid Waste Acceptance Criteria (WAC) and follows the suggested outline provided by WHC in the letter of April 26, 1990, to Dr. R.H. Thomas, Occupational Health Division, LBL. LLW is to be transferred to the WHC Hanford Site Central Waste Complex and Burial Grounds in Hanford, Washington.

  10. Micromanagement of Immune System: Role of miRNAs in Helminthic Infections.

    Science.gov (United States)

    Arora, Naina; Tripathi, Shweta; Singh, Aloukick K; Mondal, Prosenjit; Mishra, Amit; Prasad, Amit

    2017-01-01

    Helminthic infections fall under neglected tropical diseases, although they inflict severe morbidity to human and causes major economic burden on health care system in many developing countries. There is increased effort to understand their immunopathology in recent days due to their immuno-modulatory capabilities. Immune response is primarily controlled at the transcriptional level, however, microRNA-mediated RNA interference is emerging as important regulatory machinery that works at the translation level. In the past decade, microRNA (miRNA/miR) research has advanced with significant momentum. The result is ever increasing list of curated sequences from a broad panel of organisms including helminths. Several miRNAs had been discovered from trematodes, nematodes and cestodes like let-7, miR155, miR-199, miR-134, miR-223, miR-146, and fhe-mir-125a etc., with potential role in immune modulation. These miRs had been associated with TGF-β, MAPK, Toll-like receptor, PI3K/AKT signaling pathways and insulin growth factor regulation. Thus, controlling the immune cells development, survival, proliferation and death. Apart from micromanagement of immune system, they also express certain unique miRNA also like cis- miR-001, cis- miR-2, cis- miR-6, cis- miR-10, cis- miR-18, cis- miR-19, trs-mir-0001, fhe-miR-01, fhe-miR-07, fhe-miR-08, egr-miR-4988, egr-miR-4989 etc. The specific role played by most of these species specific unique miRs are yet to be discovered. However, these newly discovered miRNAs might serve as novel targets for therapeutic intervention or biomarkers for parasitic infections.

  11. Targeting miR-155 to Treat Experimental Scleroderma.

    Science.gov (United States)

    Yan, Qingran; Chen, Jie; Li, Wei; Bao, Chunde; Fu, Qiong

    2016-02-01

    Scleroderma is a refractory autoimmune skin fibrotic disorder. Alterations of microRNAs in lesional skin could be a new approach to treating the disease. Here, we found that expression of miR-155 was up regulated in lesional skin tissue from patients with either systemic or localized scleroderma, and correlated with fibrosis area. Then we demonstrated the potential of miR-155 as a therapeutic target in pre-clinical scleroderma models. MiR-155(-/-) mice were resistant to bleomycin induced skin fibrosis. Moreover, topical antagomiR-155 could effectively treat mice primed with subcutaneous bleomycin. In primary skin fibroblast, miR-155 silencing could inhibit collagen synthesis function, as well as signaling intensity of two pro-fibrotic pathways, Wnt/β-catenin and Akt, simultaneously. We further showed that miR-155 could regulate the two pathways via directly targeting casein kinase 1α (CK1α) and Src homology 2-containing inositol phosphatase-1 (SHIP-1), as previous reports. Mice with miR-155 knockout or topical antagomir-155 treatment showed inhibited Wnt/β-catenin and Akt signaling in skin upon bleomycin challenge. Together, our data suggest the potential of miR-155 silencing as a promising treatment for dermal fibrosis, especially in topical applications.

  12. The Aspiring Adept: Robert Boyle and His Alchemical Quest (Lawrence M. Principe)

    Science.gov (United States)

    Kovac, Jeffrey

    1999-10-01

    Robert Boyle is widely regarded as the Father of Modern Chemistry, who broke once and for all from the irrational, misguided alchemy that preceded him. One of the goals of this carefully researched and argued new book by Lawrence M. Principe, Assistant Professor in the Department of Chemistry and the Institute for the History of Science, Medicine, and Technology at The Johns Hopkins University, is to refute the two errors in this characterization of Boyle and to understand his life, thought, and work in the intellectual and social context of his time. This book is not for the casual reader; it is a detailed scholarly treatise in the history of science, but it provides a fresh and interesting perspective on Boyle and on the development of chemistry in the 17th century. Boyle is usually characterized as a modern scientist and his most famous book, The Skeptical Chymist, as a critique of traditional alchemy. Principe demonstrates that this characterization is based on a selective and sometimes incorrect reading of Boyle's works. Like Newton, Boyle was deeply involved in traditional transmutational alchemy, reading the works of other alchemists, performing experiments, and even witnessing transmutations. Alchemy, however, was not a monolith and Boyle adhered to what Principe tentatively identifies as a uniquely English school of supernatural alchemy. According to Principe, The Skeptical Chymist was mainly a criticism of the Paracelsians interested in chemical medicine rather than a defense of what we would now regard as modern chemistry. To further support his characterization of Boyle and to better reveal Boyle's involvement in alchemyparticularly the transmutation of base metals to gold, termed chrysopoeiaPrincipe has reconstructed from some 20 fragments one of Boyle's alchemical manuscripts, his Dialogue on the Transmutation of Metals. The full text of this lost work is included as Appendix 1. Two other primary sources, Interview Accounts of Transmutations and

  13. Low-Level Plutonium Bioassay Measurements at the Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Hamilton, T; Brown, T; Hickman, D; Marchetti, A; Williams, R; Kehl, S

    2007-06-18

    Plutonium-239 ({sup 239}Pu) and plutonium-240 ({sup 240}Pu) are important alpha emitting radionuclides contained in radioactive debris from nuclear weapons testing. {sup 239}Pu and {sup 240}Pu are long-lived radionuclides with half-lives of 24,400 years and 6580 years, respectively. Concerns over human exposure to plutonium stem from knowledge about the persistence of plutonium isotopes in the environment and the high relative effectiveness of alpha-radiation to cause potential harm to cells once incorporated into the human body. In vitro bioassay tests have been developed to assess uptakes of plutonium based on measured urinary excretion patterns and modeled metabolic behaviors of the absorbed radionuclides. Systemic plutonium absorbed by the deep lung or from the gastrointestinal tract after ingestion is either excreted or distributed to other organs, primarily to the liver and skeleton, where it is retained for biological half-times of around 20 and 50 years, respectively. Dose assessment and atoll rehabilitation programs in the Marshall Islands have historically given special consideration to residual concentrations of plutonium in the environment even though the predicted dose from inhalation and/or ingestion of plutonium accounts for less than 5% of the annual effective dose from exposure to fallout contamination. Scientists from the Lawrence Livermore National Laboratory (LLNL) have developed a state-of-the-art bioassay test to assess urinary excretion rates of plutonium from Marshallese populations. This new heavy-isotope measurement system is based on Accelerator Mass Spectrometry (AMS). The AMS system at LLNL far exceeds the standard measurement requirements established under the latest United States Department of Energy (DOE) regulation, 10CFR 835, for occupational monitoring of plutonium, and offers several advantages over classical as well as competing new technologies for low-level detection and measurement of plutonium isotopes. The United States

  14. Embryonic miRNA profiles of normal and ectopic pregnancies.

    Directory of Open Access Journals (Sweden)

    Francisco Dominguez

    Full Text Available Our objective was to investigate the miRNA profile of embryonic tissues in ectopic pregnancies (EPs and controlled abortions (voluntary termination of pregnancy; VTOP. Twenty-three patients suffering from tubal EP and twenty-nine patients with a normal ongoing pregnancy scheduled for a VTOP were recruited. Embryonic tissue samples were analyzed by miRNA microarray and further validated by real time PCR. Microarray studies showed that four miRNAs were differentially downregulated (hsa-mir-196b, hsa-mir-30a, hsa-mir-873, and hsa-mir-337-3p and three upregulated (hsa-mir-1288, hsa-mir-451, and hsa-mir-223 in EP compared to control tissue samples. Hsa-miR-196, hsa-miR-223, and hsa-miR-451 were further validated by real time PCR in a wider population of EP and control samples. We also performed a computational analysis to identify the gene targets and pathways which might be modulated by these three differentially expressed miRNAs. The most significant pathways found were the mucin type O-glycan biosynthesis and the ECM-receptor-interaction pathways. We also checked that the dysregulation of these three miRNAs was able to alter the expression of the gene targets in the embryonic tissues included in these pathways such as GALNT13 and ITGA2 genes. In conclusion, analysis of miRNAs in ectopic and eutopic embryonic tissues shows different expression patterns that could modify pathways which are critical for correct implantation, providing new insights into the understanding of ectopic implantation in humans.

  15. miR-92a family and their target genes in tumorigenesis and metastasis

    Energy Technology Data Exchange (ETDEWEB)

    Li, Molin, E-mail: molin_li@hotmail.com [Department of Pathophysiology, Basic Medical Science of Dalian Medical University, Dalian 116044 (China); Institute of Cancer Stem Cell, Dalian Medical University Cancer Center, Dalian 116044 (China); Guan, Xingfang; Sun, Yuqiang [Department of Pathophysiology, Basic Medical Science of Dalian Medical University, Dalian 116044 (China); Mi, Jun [Institute of Cancer Stem Cell, Dalian Medical University Cancer Center, Dalian 116044 (China); Shu, Xiaohong [College of Pharmacy, Dalian Medical University Cancer Center, Dalian 116044 (China); Liu, Fang [Department of Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian 116027 (China); Li, Chuangang, E-mail: li_chuangang@sina.com [Department of Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian 116027 (China)

    2014-04-15

    The miR-92a family, including miR-25, miR-92a-1, miR-92a-2 and miR-363, arises from three different paralog clusters miR-17-92, miR-106a-363, and miR-106b-25 that are highly conservative in the process of evolution, and it was thought as a group of microRNAs (miRNAs) correlated with endothelial cells. Aberrant expression of miR-92a family was detected in multiple cancers, and the disturbance of miR-92a family was related with tumorigenesis and tumor development. In this review, the progress on the relationship between miR-92a family and their target genes and malignant tumors will be summarized. - Highlights: • Aberrant expression of miR-92a, miR-25 and miR-363 can be observed in many kinds of malignant tumors. • The expression of miR-92a family is regulated by LOH, epigenetic alteration, transcriptional factors such as SP1, MYC, E2F, wild-type p53 etc. • Roles of miR-92a family in tumorigenesis and development: promoting cell proliferation, invasion and metastasis, inhibiting cell apoptosis.

  16. miR-92a family and their target genes in tumorigenesis and metastasis

    International Nuclear Information System (INIS)

    Li, Molin; Guan, Xingfang; Sun, Yuqiang; Mi, Jun; Shu, Xiaohong; Liu, Fang; Li, Chuangang

    2014-01-01

    The miR-92a family, including miR-25, miR-92a-1, miR-92a-2 and miR-363, arises from three different paralog clusters miR-17-92, miR-106a-363, and miR-106b-25 that are highly conservative in the process of evolution, and it was thought as a group of microRNAs (miRNAs) correlated with endothelial cells. Aberrant expression of miR-92a family was detected in multiple cancers, and the disturbance of miR-92a family was related with tumorigenesis and tumor development. In this review, the progress on the relationship between miR-92a family and their target genes and malignant tumors will be summarized. - Highlights: • Aberrant expression of miR-92a, miR-25 and miR-363 can be observed in many kinds of malignant tumors. • The expression of miR-92a family is regulated by LOH, epigenetic alteration, transcriptional factors such as SP1, MYC, E2F, wild-type p53 etc. • Roles of miR-92a family in tumorigenesis and development: promoting cell proliferation, invasion and metastasis, inhibiting cell apoptosis

  17. Mercury transport between sediments and the overlying water of the St. Lawrence River area of concern near Cornwall, Ontario

    Energy Technology Data Exchange (ETDEWEB)

    Delongchamp, Tania M., E-mail: tdelongchamp@intrinsikscience.co [Department of Biology, University of Ottawa, 150 Louis Pasteur, Ottawa, Ontario K1N 6N5 (Canada); Ridal, Jeffrey J. [St. Lawrence River Institute of Environmental Sciences, 2 Belmont Street, Cornwall, Ontario, K6H 4Z1 (Canada); Lean, David R.S. [Department of Biology, University of Ottawa, 150 Louis Pasteur, Ottawa, Ontario K1N 6N5 (Canada); Poissant, Laurier [Meteorological Service of Canada, Atmospheric Toxic Processes Section, Environment Canada, 105 McGill 7th floor (Youville), Montreal, Quebec H2Y 2E7 (Canada); Blais, Jules M., E-mail: jules.blais@uottawa.c [Department of Biology, University of Ottawa, 150 Louis Pasteur, Ottawa, Ontario K1N 6N5 (Canada)

    2010-05-15

    Contaminated sediments in the St. Lawrence River remain a difficult problem despite decreases in emissions. Here, sediment and pore water phases were analyzed for total mercury (THg) and methyl mercury (MeHg) and diffusion from the sediment to the overlying water was 17.5 +- 10.6 SE ng cm{sup -2} yr{sup -1} for THg and 3.8 +- 1.7 SE ng cm{sup -2} yr{sup -1} for MeHg. These fluxes were very small when compared to the particle-bound mercury flux accumulating in the sediment (183 +- 30 SE ng cm{sup -2} yr{sup -1}). Studies have reported that fish from the westernmost site have higher Hg concentrations than fish collected from the other two sites of the Cornwall Area of Concern, which could not be explained by differences in the Hg flux or THg concentrations in sediments, but the highest concentrations of sediment MeHg, and the greatest proportions of MeHg to THg in both sediment and pore water were observed where fish had highest MeHg concentrations. - Sediments in the St. Lawrence area of concern near Cornwall are a net sink for mercury.

  18. A Monte Carlo Simulation of the in vivo measurement of lung activity in the Lawrence Livermore National Laboratory torso phantom.

    Science.gov (United States)

    Acha, Robert; Brey, Richard; Capello, Kevin

    2013-02-01

    A torso phantom was developed by the Lawrence Livermore National Laboratory (LLNL) that serves as a standard for intercomparison and intercalibration of detector systems used to measure low-energy photons from radionuclides, such as americium deposited in the lungs. DICOM images of the second-generation Human Monitoring Laboratory-Lawrence Livermore National Laboratory (HML-LLNL) torso phantom were segmented and converted into three-dimensional (3D) voxel phantoms to simulate the response of high purity germanium (HPGe) detector systems, as found in the HML new lung counter using a Monte Carlo technique. The photon energies of interest in this study were 17.5, 26.4, 45.4, 59.5, 122, 244, and 344 keV. The detection efficiencies at these photon energies were predicted for different chest wall thicknesses (1.49 to 6.35 cm) and compared to measured values obtained with lungs containing (241)Am (34.8 kBq) and (152)Eu (10.4 kBq). It was observed that no statistically significant differences exist at the 95% confidence level between the mean values of simulated and measured detection efficiencies. Comparisons between the simulated and measured detection efficiencies reveal a variation of 20% at 17.5 keV and 1% at 59.5 keV. It was found that small changes in the formulation of the tissue substitute material caused no significant change in the outcome of Monte Carlo simulations.

  19. Estimate of aircraft crash hit frequencies on to facilities at the Lawrence Livermore National Laboratory (LLNL) Site 200

    International Nuclear Information System (INIS)

    Kimura, C.Y.

    1997-01-01

    Department of Energy (DOE) nuclear facilities are required by DOE Order 5480.23, Section 8.b.(3)(k) to consider external events as initiating events to accidents within the scope of their Safety Analysis Reports (SAR). One of the external initiating events which should be considered within the scope of a SAR is an aircraft accident, i.e., an aircraft crashing into the nuclear facility with the related impact and fire leading to penetration of the facility and to the release of radioactive and/or hazardous materials. This report presents the results of an Aircraft Crash Frequency analysis performed for the Materials Management Area (MMA), and the National Ignition Facility (NIF) at the Lawrence Livermore National Laboratory (LLNL) Site 200. The analysis estimates only the aircraft crash hit frequency on to the analyzed facilities. No initial aircraft crash hit frequency screening structural response calculations of the facilities to the aircraft impact, or consequence analysis of radioactive/hazardous materials released following the aircraft impact are performed. The method used to estimate the aircraft crash hit frequencies on to facilities at the Lawrence Livermore National Laboratory (LLNL) generally follows the procedure given by the DOE Standard 3014-96 on Aircraft Crash Analysis. However, certain adjustments were made to the DOE Standard procedure because of the site specific fight environment or because of facility specific characteristics

  20. Supplement analysis for continued operation of Lawrence Livermore National Laboratory and Sandia National Laboratories, Livermore. Volume 2: Comment response document

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-03-01

    The US Department of Energy (DOE), prepared a draft Supplement Analysis (SA) for Continued Operation of Lawrence Livermore National Laboratory (LLNL) and Sandia National Laboratories, Livermore (SNL-L), in accordance with DOE`s requirements for implementation of the National Environmental Policy Act of 1969 (NEPA) (10 Code of Federal Regulations [CFR] Part 1021.314). It considers whether the Final Environmental Impact Statement and Environmental Impact Report for Continued Operation of Lawrence Livermore National Laboratory and Sandia National Laboratories, Livermore (1992 EIS/EIR) should be supplement3ed, whether a new environmental impact statement (EIS) should be prepared, or no further NEPA documentation is required. The SA examines the current project and program plans and proposals for LLNL and SNL-L, operations to identify new or modified projects or operations or new information for the period from 1998 to 2002 that was not considered in the 1992 EIS/EIR. When such changes, modifications, and information are identified, they are examined to determine whether they could be considered substantial or significant in reference to the 1992 proposed action and the 1993 Record of Decision (ROD). DOE released the draft SA to the public to obtain stakeholder comments and to consider those comments in the preparation of the final SA. DOE distributed copies of the draft SA to those who were known to have an interest in LLNL or SNL-L activities in addition to those who requested a copy. In response to comments received, DOE prepared this Comment Response Document.

  1. Reconnaissance investigation of high-calcium marble in the Beaver Creek area, St. Lawrence County, New York

    Science.gov (United States)

    Brown, C. Ervin

    1978-01-01

    Three belts of marble of the Grenville Series were mapped in the Beaver Creek drainage basin, St. Lawrence County, N.Y. One of these, on the west side of Beaver Creek, consists of coarsely crystalline pure calcitic marble that occurs in a zone at least 10 by 0.8 km in extent. Samples of marble show CaCO3 content to be greater than 93 percent, and some samples contain greater than 96 percent, and only small amounts of MgO and Fe203 are present. Marble in two other belts to the east of Beaver Creek are variable in composition, but locally have high content of calcium carbonate material. The marble deposit west of Beaver Creek has a chemical composition favorable for specialized chemical, industrial, and metallurgical uses. Another favorable aspect of the deposit is its proximity to inexpensive water transportation on the St. Lawrence Seaway only 27.5 km away by road, at Ogdensburg, N.Y.

  2. Assessing condition of macroinvertebrate communities and sediment toxicity in the St. Lawrence River at Massena Area-of-Concern

    Science.gov (United States)

    Duffy, Brian T.; Baldigo, Barry P.; Smith, Alexander J.; George, Scott D.; David, Anthony M.

    2016-01-01

    In 1972, the USA and Canada agreed to restore the chemical, physical, and biological integrity of the Great Lakes ecosystem under the first Great Lakes Water Quality Agreement. In subsequent amendments, part of the St. Lawrence River at Massena, New York and segments of three tributaries, were designated as an Area of Concern (AOC) due to the effects of polychlorinated biphenyls (PCBs), lead and copper contamination, and habitat degradation and resulting impairment to several beneficial uses. Because sediments have been largely remediated, the present study was initiated to evaluate the current status of the benthic macroinvertebrate (benthos) beneficial use impairment (BUI). Benthic macroinvertebrate communities and sediment toxicity tests using Chironomus dilutus were used to test the hypotheses that community condition and sediment toxicity at AOC sites were not significantly different from those of adjacent reference sites. Grain size was found to be the main driver of community composition and macroinvertebrate assemblages, and bioassessment metrics did not differ significantly between AOC and reference sites of the same sediment class. Median growth of C. dilutus and its survival in three of the four river systems did not differ significantly in sediments from AOC and reference sites. Comparable macroinvertebrate assemblages and general lack of toxicity across most AOC and reference sites suggest that the quality of sediments should not significantly impair benthic macroinvertebrate communities in most sites in the St. Lawrence River AOC.

  3. Particle size fractionation of paralytic shellfish toxins (PSTs): seasonal distribution and bacterial production in the St Lawrence estuary, Canada.

    Science.gov (United States)

    Michaud, S; Levasseur, M; Doucette, G; Cantin, G

    2002-10-01

    We determined the seasonal distribution of paralytic shellfish toxins (PSTs) and PST producing bacteria in > 15, 5-15, and 0.22-5 microm size fractions in the St Lawrence. We also measured PSTs in a local population of Mytilus edulis. PST concentrations were determined in each size fraction and in laboratory incubations of sub-samples by high performance liquid chromatography (HPLC), including the rigorous elimination of suspected toxin 'imposter' peaks. Mussel toxin levels were determined by mouse bioassay and HPLC. PSTs were detected in all size fractions during the summer sampling season, with 47% of the water column toxin levels associated with particles smaller than Alexandrium tamarense ( 15 microm size fraction, we estimated that as much as 92% of PSTs could be associated with particles other than A. tamarense. Our results stress the importance of taking into account the potential presence of PSTs in size fractions other than that containing the known algal producer when attempting to model shellfish intoxication, especially during years of low cell abundance. Finally, our HPLC results confirmed the presence of bacteria capable of autonomous PST production in the St Lawrence as well as demonstrating their regular presence and apparent diversity in the plankton. Copyright 2002 Elsevier Science Ltd.

  4. SCR series switch and impulse crowbar at the Lawrence Berkeley Laboratory for CTR neutral beam source development

    International Nuclear Information System (INIS)

    Franck, J.V.; Arthur, A.A.; Brusse, L.A.; Low, W.

    1977-10-01

    The series switch is designed to operate at 120kV and pass 65A for 0.5 sec every 30 sec on the Lawrence Berkeley Laboratory CTR Neutral Beam Source Test Stand IIIB. The series switch consists of 400 individual SCR circuits connected in series and is turned on by a simple system of cascaded pulse transformers with multiple single turn secondaries each driving the individual SCR gates. It is turned off by an SCR impulse crowbar that momentarily shorts the power supply allowing the series switch to recover. The SCR switch has been tested in the impulse crowbar configuration and will reliably commutate up to 90A at 120kV. The series switch and impulse crowbar are now in service in Test Stand IIIB. A series switch and impulse crowbar similar in concept is routinely powering a 10 x 10 cm source at 150kV, 20A, 0.5 sec with a 1% duty cycle on the Lawrence Berkeley Laboratory CTR NSB Test Stand IIIA

  5. Improving regional climate and hydrological forecasting following the record setting flooding across the Lake Ontario - St. Lawrence River system

    Science.gov (United States)

    Gronewold, A.; Seglenieks, F.; Bruxer, J.; Fortin, V.; Noel, J.

    2017-12-01

    In the spring of 2017, water levels across Lake Ontario and the upper St. Lawrence River exceeded record high levels, leading to widespread flooding, damage to property, and controversy over regional dam operating protocols. Only a few years earlier, water levels on Lakes Superior, Michigan, and Huron (upstream of Lake Ontario) had dropped to record low levels leading to speculation that either anthropogenic controls or climate change were leading to chronic water loss from the Great Lakes. The contrast between low water level conditions across Earth's largest lake system from the late 1990s through 2013, and the rapid rise prior to the flooding in early 2017, underscores the challenges of quantifying and forecasting hydrologic impacts of rising regional air and water temperatures (and associated changes in lake evaporation) and persistent increases in long-term precipitation. Here, we assess the hydrologic conditions leading to the recent record flooding across the Lake Ontario - St. Lawrence River system, with a particular emphasis on understanding the extent to which those conditions were consistent with observed and anticipated changes in historical and future climate, and the extent to which those conditions could have been anticipated through improvements in seasonal climate outlooks and hydrological forecasts.

  6. Corrosion of mineral insulated (MI) cables at MAPS-2

    International Nuclear Information System (INIS)

    Bora, J.S.; Babar, A.K.

    1989-01-01

    It has been experimentally verified that the cause of undesirable behaviour of mineral insulated (MI) cables at Madras Atomic Power Station Unit 2 (MAPS-2) is due to corrosion of termination. It is always possible to restore them to their normal condition by heating if degradation is due to absorption of moisture and by cutting and removing the affected portion in case of short or open failures. During extended shutdown, it is advisable to check other MI terminations and take appropriate corrective action in order to prevent failures in future. During installation MI Cables are to be heated before sealing and should never be heated after sealing. (author)

  7. Circulating miR-126 and miR-499 reflect progression of cardiovascular disease; correlations with uric acid and ejection fraction

    Directory of Open Access Journals (Sweden)

    Masoud Khanaghaei

    2016-04-01

    Full Text Available BackgroundThe aim of this study was to assess plasma levels of endothelium- and heart-associated microRNAs (miRNAs miR-126 and miR-499, respectively, using quantitative reverse transcriptase polymerase chain reaction.MethodsA two-step analysis was conducted on 75 patients undergoing off-pomp coronary artery bypass graft (CABG surgery. Five biomarkers of inflammation and cardiac injury were assessed in addition to the above-mentioned miRNAs.ResultsPlasma concentrations of miRNAs were found to be significantly correlated with plasma levels of cardiac troponin I (cTnI (miR-499, r 0.49, p~0.002; miR-126, r = 0.30, p~0.001, indicating cardiac damage. Data analysis revealed that miR-499 had higher sensitivity and specificity for cardiac injury than miR-126, which reflects more endothelial activation. Interestingly, a strong correlation was observed between both miRNAs and uric acid (UA levels with ventricular contractility measured as ejection fraction (EF (miR-499/EF%, r = 0.58, p~0.004; UA/EF%, r = -0.6, p~0.006; UA/miR-499, r = -0.34; UA/miR-126, r = 0.5, p~0.01.ConclusionsIn patients undergoing CABG, circulating miR-126/499 is associated with presentation of traditional risk factors and reflects post-operative response to injury. Plasma pool of miRNAs likely reflects extracellular miRNAs which are proportional to intracellular miRNA levels. Therefore, circulating levels of these miRNAs have prognostic implications in detection of higher risk of future cardiovascular events.

  8. miR-184 and miR-150 promote renal glomerular mesangial cell aging by targeting Rab1a and Rab31.

    Science.gov (United States)

    Liu, Xiujuan; Fu, Bo; Chen, Dapeng; Hong, Quan; Cui, Jing; Li, Jin; Bai, Xueyuan; Chen, Xiangmei

    2015-08-15

    The molecular mechanism of kidney aging is not well understood, but the abnormal expression of miRNAs with aging is considered to be an important contributor. miR-184 and miR-150 were screened using a miRNA microarray and qRT-PCR and found to be significantly upregulated in 24-month-old rats. Rat renal primary glomerular mesangial cells (GMCs) were isolated from 3-month and 24-month-old rats for the in vitro analysis of the roles of miR-184 and miR-150 in kidney aging. Bioinformatics analyses suggested that Rab1a and Rab31, which are associated with cell autophagy, were targeted by both miR-184 and miR-150. miR-184 and miR-150 were increased significantly in aging GMCs versus young cells, while Rab1a and Rab31 were significantly lower in aging cells. Furthermore, dual luciferase reporter assays revealed that miR-184 and miR-150 bound to the 3'-UTR of Rab1a and Rab31 mRNAs. Transfection of miR-184 and miR-150 mimics into young GMCs suppressed the expression of Rab1a and Rab31. Transfected cells showed lower autophagy activities and higher levels of cellular oxidative products, leading to the aging of young GMCs. However, miR-184 and miR-150 inhibitors promoted autophagy and reduced oxidative damage by upregulating Rab1a and Rab31 in old GMCs. In conclusion, miR-184 and miR-150 inhibited autophagy, promoting GMC aging. Copyright © 2015 Elsevier Inc. All rights reserved.

  9. Serum miRNAs miR-23a, 206, and 499 as Potential Biomarkers for Skeletal Muscle Atrophy

    Directory of Open Access Journals (Sweden)

    Fei Wang

    2017-01-01

    Full Text Available Muscle biopsy has long been expected to be replaced by noninvasive biomarkers with diagnostic value and prognostic applications for muscle atrophy. Growing evidence suggests that circulating microRNAs (miRNAs could act as biomarkers for numerous pathophysiological statuses. In the present study, our results showed that the serum levels of six muscle-specific miRNAs (miR-1/23a/133/206/208b/499 were all elevated in unloading induced mice. The medium levels of these six muscle-specific miRNAs were all elevated in starvation induced atrophic C2C12 myotubes. Moreover, the serum levels of miR-23a/206/499 were induced in participants after 45 days of head-down bed rest (HDBR. The levels of miR-23a/206/499 were positively correlated with the ratio of soleus volume loss in HDBR participants, indicating that they might represent the process of muscle loss. In conclusion, our results demonstrated that circulating miRNAs could serve as useful biochemical and molecular indicators for muscle atrophy diagnosis and disease progression.

  10. Circulating miR-765 and miR-149: Potential Noninvasive Diagnostic Biomarkers for Geriatric Coronary Artery Disease Patients

    Directory of Open Access Journals (Sweden)

    Md Sayed Ali Sheikh

    2015-01-01

    Full Text Available The purpose of this study was to evaluate the diagnostic value of circulating miR-765 and miR-149 as noninvasive early biomarkers for geriatric coronary artery disease (CAD patients. A total of 69 angiographically documented CAD patients including 37 stable CAD (72.9 ± 4.2 years and 32 unstable CAD (72.03 ± 4.3 years and 20 healthy subjects (71.7 ± 5.2 years, matched for age, sex, smoking habit, hypertension, and diabetes, were enrolled in this study. Compared with healthy subjects, circulating miR-765 levels were increased by 2.9-fold in stable CAD and 5.8-fold in unstable CAD patients, respectively, while circulating miR-149 levels were downregulated by 3.5-fold in stable CAD and 4.2-fold in unstable CAD patients, respectively. Furthermore, plasma levels of miR-765 were found to be positively correlated with ages within control, stable, and unstable groups. The ROC curves of miR-765 and miR-149 represented significant diagnostic values with an area under curve (AUC of 0.959, 0.972 and 0.938, 0.977 in stable CAD patients and unstable CAD patients as compared with healthy subjects, respectively. Plasma levels of miR-765 and miR-149 might be used as noninvasive biomarkers for the diagnosis of CAD in geriatric people.

  11. Involvement of miR160/miR393 and their targets in cassava responses to anthracnose disease.

    Science.gov (United States)

    Pinweha, Nattaya; Asvarak, Thipa; Viboonjun, Unchera; Narangajavana, Jarunya

    2015-02-01

    Cassava is a starchy root crop for food and industrial applications in many countries around the world. Among the factors that affect cassava production, diseases remain the major cause of yield loss. Cassava anthracnose disease is caused by the fungus Colletotrichum gloeosporioides. Severe anthracnose attacks can cause tip die-backs and stem cankers, which can affect the availability of planting materials especially in large-scale production systems. Recent studies indicate that plants over- or under-express certain microRNAs (miRNAs) to cope with various stresses. Understanding how a disease-resistant plant protects itself from pathogens should help to uncover the role of miRNAs in the plant immune system. In this study, the disease severity assay revealed different response to C. gloeosporioides infection in two cassava cultivars. Quantitative RT-PCR analysis uncovered the differential expression of the two miRNAs and their target genes in the two cassava cultivars that were subjected to fungal infection. The more resistant cultivar revealed the up-regulation of miR160 and miR393, and consequently led to low transcript levels in their targets, ARF10 and TIR1, respectively. The more susceptible cultivar exhibited the opposite pattern. The cis-regulatory elements relevant to defense and stress responsiveness, fungal elicitor responsiveness and hormonal responses were the most prevalent present in the miRNAs gene promoter regions. The possible dual role of these specific miRNAs and their target genes associated with cassava responses to C. gloeosporioides is discussed. This is the first study to address the molecular events by which miRNAs which might play a role in fungal-infected cassava. A better understanding of the functions of miRNAs target genes should greatly increase our knowledge of the mechanism underlying susceptibility and lead to new strategies to enhance disease tolerance in this economically important crop. Copyright © 2014 Elsevier GmbH. All

  12. Preparedness activities regarding the protection of public health in case of a major oil or chemical spill on the St-Lawrence River

    International Nuclear Information System (INIS)

    Guerrier, P.; Manon, P.

    1996-01-01

    A project was initiated to protect the health of people living in the St-Lawrence basin from contaminants associated with an oil or chemical spill. Between 1980 and 1990, more than 240 chemical spills and 300 oil spills were recorded in the region which has prompted concerns regarding the possible contamination of drinking water sources. 45% of Quebec's population relies on the St-Lawrence River as a source of drinking water. Thus far, the project has identified the major chemical and oil products transported on the St-Lawrence River, and the main health risks associated with these products. Computerized dispersion models which can determine the migration of the contaminants in water, are available. Simulation exercises have been carried out to train personnel in the event of an actual spill. 1 ref

  13. Public health risks associated with oil and chemical spills in cold freshwater environments: a simulation exercise involving a phenol and diesel spill in the St. Lawrence River

    International Nuclear Information System (INIS)

    Lefaivre, D.; Jarry, V.; Guerrier, P.; Paul, M.; Colliou, M.

    1997-01-01

    The St. Lawrence River is the source of drinking water for some 45 per cent of the population in the Province of Quebec, hence contamination of the river by oil or chemical spills is a matter of great public health importance. Project SHORES was developed by the Quebec Environmental Health Committee through the St. Lawrence 'Vision 2000' Action Plan. As part of this project, a simulation exercise involving phenol and diesel fuel was carried out. The exercise included development of a computerized dispersion model which was then used to evaluate the migration of phenol in critical areas of the St. Lawrence River. Main public health risks to nearby populations, with emphasis on drinking water contamination, were assessed based on the simulation results. 18 refs., 2 tabs. 1 fig

  14. N6-adenosine methylation in MiRNAs.

    Directory of Open Access Journals (Sweden)

    Tea Berulava

    Full Text Available Methylation of N6-adenosine (m6A has been observed in many different classes of RNA, but its prevalence in microRNAs (miRNAs has not yet been studied. Here we show that a knockdown of the m6A demethylase FTO affects the steady-state levels of several miRNAs. Moreover, RNA immunoprecipitation with an anti-m6A-antibody followed by RNA-seq revealed that a significant fraction of miRNAs contains m6A. By motif searches we have discovered consensus sequences discriminating between methylated and unmethylated miRNAs. The epigenetic modification of an epigenetic modifier as described here adds a new layer to the complexity of the posttranscriptional regulation of gene expression.

  15. Circulating miRNAs as biomarkers for endocrine disorders.

    Science.gov (United States)

    Butz, H; Kinga, N; Racz, K; Patocs, A

    2016-01-01

    Specific, sensitive and non-invasive biomarkers are always needed in endocrine disorders. miRNAs are short, non-coding RNA molecules with well-known role in gene expression regulation. They are frequently dysregulated in metabolic and endocrine diseases. Recently it has been shown that they are secreted into biofluids by nearly all kind of cell types. As they can be taken up by other cells they may have a role in a new kind of paracrine, cell-to-cell communication. Circulating miRNAs are protected by RNA-binding proteins or microvesicles hence they can be attractive candidates as diagnostic or prognostic biomarkers. In this review, we summarize the characteristics of extracellular miRNA's and our knowledge about their origin and potential roles in endocrine and metabolic diseases. Discussions about the technical challenges occurring during identification and measurement of extracellular miRNAs and future perspectives about their roles are also highlighted.

  16. miRNA delivery for skin wound healing.

    Science.gov (United States)

    Meng, Zhao; Zhou, Dezhong; Gao, Yongsheng; Zeng, Ming; Wang, Wenxin

    2017-12-19

    The wound healing has remained a worldwide challenge as one of significant public health problems. Pathological scars and chronic wounds caused by injury, aging or diabetes lead to impaired tissue repair and regeneration. Due to the unique biological wound environment, the wound healing is a highly complicated process, efficient and targeted treatments are still lacking. Hence, research-driven to discover more efficient therapeutics is a highly urgent demand. Recently, the research results have revealed that microRNA (miRNA) is a promising tool in therapeutic and diagnostic fields because miRNA is an essential regulator in cellular physiology and pathology. Therefore, new technologies for wound healing based on miRNA have been developed and miRNA delivery has become a significant research topic in the field of gene delivery. Copyright © 2017. Published by Elsevier B.V.

  17. Operation of the NuMI Beam Monitoring System

    International Nuclear Information System (INIS)

    Zwaska, Robert M.; Indurthy, Dharma; Keisler, Ryan; Kopp, Sacha; Mendoza, Steven; Pavlovich, Zarko; Proga, Marek; Bishai, Mary; Diwan, Milind; Viren, Brett; Harris, Debbie; Marchionni, Alberto; Morfin, Jorge; McDonald, Jeffrey; Naples, Donna; Northacker, David; Erwin, Albert; Ping, Huican; Velissaris, Cristos

    2006-01-01

    The NuMI (Neutrinos at the Main Injector) facility produces an intense neutrino beam for experiments. The NuMI Beam Monitoring system consists of four arrays of ion chambers that measure the intensity and distribution of the remnant hadron and tertiary muon beams produced in association with the neutrinos. The ion chambers operate in an environment of high particle fluxes and high radiation

  18. Badania nad komunikacją międzykulturową

    DEFF Research Database (Denmark)

    Wilczewski, Michał; Søderberg, Anne-Marie

    2017-01-01

    jako badanie narracyjne. Proponujemy, by podejście narracyjne zostało wykorzystane w badaniach nad komunikacją międzykulturową, gdyż oferuje narzędzia dające dostęp do sposobów, w jakie uczestnicy komunikacji opowiadają o swoich międzykulturowych doświadczeniach, do refleksji na ich temat, więc metoda...

  19. Exploring the miRNA regulatory network using evolutionary correlations.

    Directory of Open Access Journals (Sweden)

    Benedikt Obermayer

    2014-10-01

    Full Text Available Post-transcriptional regulation by miRNAs is a widespread and highly conserved phenomenon in metazoans, with several hundreds to thousands of conserved binding sites for each miRNA, and up to two thirds of all genes under miRNA regulation. At the same time, the effect of miRNA regulation on mRNA and protein levels is usually quite modest and associated phenotypes are often weak or subtle. This has given rise to the notion that the highly interconnected miRNA regulatory network exerts its function less through any individual link and more via collective effects that lead to a functional interdependence of network links. We present a Bayesian framework to quantify conservation of miRNA target sites using vertebrate whole-genome alignments. The increased statistical power of our phylogenetic model allows detection of evolutionary correlation in the conservation patterns of site pairs. Such correlations could result from collective functions in the regulatory network. For instance, co-conservation of target site pairs supports a selective benefit of combinatorial regulation by multiple miRNAs. We find that some miRNA families are under pronounced co-targeting constraints, indicating a high connectivity in the regulatory network, while others appear to function in a more isolated way. By analyzing coordinated targeting of different curated gene sets, we observe distinct evolutionary signatures for protein complexes and signaling pathways that could reflect differences in control strategies. Our method is easily scalable to analyze upcoming larger data sets, and readily adaptable to detect high-level selective constraints between other genomic loci. We thus provide a proof-of-principle method to understand regulatory networks from an evolutionary perspective.

  20. The miR-10 microRNA precursor family

    DEFF Research Database (Denmark)

    Tehler, Disa; Høyland-Kroghsbo, Nina Molin; Lund, Anders H

    2011-01-01

    The miR-10 microRNA precursor family encodes a group of short non-coding RNAs involved in gene regulation. The miR-10 family is highly conserved and has sparked the interest of many research groups because of the genomic localization in the vicinity of, coexpression with and regulation of the Hox...... gene developmental regulators. Here, we review the current knowledge of the evolution, physiological function and involvement in cancer of this family of microRNAs....