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Sample records for monroe mi lawrence

  1. Quarry Quest. A Field Trip Guide to the Indiana Limestone District, Monroe and Lawrence Counties, Indiana.

    Science.gov (United States)

    Shewmaker, Sherman N.

    This guide provides information for planning a field trip to the Indiana Limestone District. This district, located in Monroe and Lawrence Counties, Indiana, is responsible for material that has dominated the building-limestone market in the United States for nearly a century. A few of the many well-known buildings using Indiana limestone are the…

  2. BASEMAP, MONROE COUNTY, MI, USA

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — FEMA Framework Basemap datasets comprise six of the seven FGDC themes of geospatial data that are used by most GIS applications (Note: the seventh framework theme,...

  3. FLOODPLAIN, MONROE COUNTY, MI, USA

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — FEMA Framework Basemap datasets comprise six of the seven FGDC themes of geospatial data that are used by most GIS applications (Note: the seventh framework theme,...

  4. Willem de Kooning: "Marilyn Monroe."

    Science.gov (United States)

    Nelson, Karen G.

    1988-01-01

    Presents a lesson plan which introduces students in grades 7-9 to Willem de Kooning and the qualities that make his oil painting "Marilyn Monroe," an example of abstract expressionist style. Includes background information on the artist and the painting as well as instructional strategies, studio activities, and evaluation procedures.…

  5. Fort Monroe Historic Image Report

    Science.gov (United States)

    2010-08-01

    ERDC/CERL SR-10-8 August 2010 Fort Monroe Historic Image Report Adam Smith , Megan Weaver Tooker, Meghan Roller, and Rory Nicholson Construction... Adam Smith was the CERL Project Manager and lead architectural historian, Megan Weaver Tooker was the lead land- scape architect, and Meghan Roller...5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S). Adam Smith , Megan Weaver Tooker, Meghan Roller and Rory Nicholson 5d. PROJECT NUMBER 5e. TASK

  6. 黑色珍珠 Earl Monroe

    Institute of Scientific and Technical Information of China (English)

    chinshen

    2009-01-01

    有人喜欢尊称他为"黑色魔术(Black Magic)",也有人习惯敬呼他"黑色耶稣(Black Jesus)"而略其名,不过蒙罗(Earl Monroe)最著名的外号,还是与他名字发音最接近的,"珍珠(Pearl)"。

  7. 75 FR 33273 - Final Environmental Impact Statement (FEIS) for Disposal and Reuse of Fort Monroe, VA

    Science.gov (United States)

    2010-06-11

    ... Department of the Army Final Environmental Impact Statement (FEIS) for Disposal and Reuse of Fort Monroe, VA... socioeconomic impacts associated with the disposal and reuse of Fort Monroe, Virginia. DATES: The waiting period... reuse of Fort Monroe. The 2005 BRAC Commission Report directed the closure of Fort Monroe and...

  8. Ernest Orlando Lawrence

    Energy Technology Data Exchange (ETDEWEB)

    Alvarez, Luis W.

    1967-02-01

    In his relatively short life of 57 years, Ernest Orlando Lawrence accomplished more than one might believe possible in a life twice as long. The important ingredients of his success were native ingenuity and basic good judgement in science, great stamina, an enthusiastic and outgoing personality, and a sense of integrity that was overwhelming. Many articles on the life and accomplishments of Ernest Lawrence have been published, and George Herbert Childs has written a book-length biography. This biographical memoir, however, has not made use of any sources other than the author's memory of Ernest Lawrence and of things learned from him. A more balanced picture will emerge when Herbert Childs biography is published; this sketch simply shows how Ernest Lawrence looked to one of his many friends.

  9. Cities, Towns and Villages, Published in unknown, Monroe County.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Cities, Towns and Villages dataset as of unknown. Data by this publisher are often provided in NAD_1983_HARN_WISCRS_Monroe_County_Feet coordinate system; in a...

  10. Michael Musto as Lindsay Lohan as Marilyn Monroe in 'The Ultimate Re-Vamping'

    National Research Council Canada - National Science Library

    Musto, Michael

    2008-01-01

    In 2008, actress Lindsay Lohan followed in the steps of her self-proclaimed idol Marilyn Monroe by doing a photo shoot with Bert Stern reminiscent of the shoot Monroe did with Stern just weeks before she died...

  11. 77 FR 60373 - Monroe Mountain Aspen Ecosystems Restoration Project Fishlake National Forest; Sevier and Piute...

    Science.gov (United States)

    2012-10-03

    ... Forest Service Monroe Mountain Aspen Ecosystems Restoration Project Fishlake National Forest; Sevier and... alternatives, within the Monroe Mountain Aspen Ecosystems Restoration Project area. The purpose of the Monroe Mountain Aspen Ecosystems Restoration Project is to implement land management activities that are...

  12. Londoni oksjoni staar oli Monroe teemantsõrmus / Arko Olesk

    Index Scriptorium Estoniae

    Olesk, Arko, 1981-

    2002-01-01

    5. augustil möödub 40 aastat Hollywoodi staari Marilyn Monroe siiani salapäraga ümbritsetud surmast. Seda arvesse võttes tõi Cooper Oweni oksjon taas välja temaga seotud esemed. Lisatud oksjonil kõige kallimalt müüdud esemete nimekiri

  13. Londoni oksjoni staar oli Monroe teemantsõrmus / Arko Olesk

    Index Scriptorium Estoniae

    Olesk, Arko, 1981-

    2002-01-01

    5. augustil möödub 40 aastat Hollywoodi staari Marilyn Monroe siiani salapäraga ümbritsetud surmast. Seda arvesse võttes tõi Cooper Oweni oksjon taas välja temaga seotud esemed. Lisatud oksjonil kõige kallimalt müüdud esemete nimekiri

  14. 76 FR 68625 - Establishment of the Fort Monroe National Monument

    Science.gov (United States)

    2011-11-07

    ...'' by Captain John Smith in 1607 when the first English colonists came to America. It was here that the... generations escaping enslavement. During the Civil War, Fort Monroe stood as a foremost Union outpost in the... freedom for thousands of enslaved people during the Civil War and served as a forerunner of...

  15. Fermi, Heisenberg y Lawrence

    Directory of Open Access Journals (Sweden)

    Ynduráin, Francisco J.

    2002-01-01

    Full Text Available Not available

    Los azares de las onomásticas hacen coincidir en este año el centenario del nacimiento de tres de los más grandes físicos del siglo XX. Dos de ellos, Fermi y Heisenberg, dejaron una marca fundamental en la ciencia (ambos, pero sobre todo el segundo y, el primero, también en la tecnología. Lawrence, indudablemente de un nivel inferior al de los otros dos, estuvo sin embargo en el origen de uno de los desarrollos tecnológicos que han sido básicos para la exploración del universo subnuclear en la segunda mitad del siglo que ha terminado hace poco, el de los aceleradores de partículas.

  16. Endoscopic excision of intraventricular neurocysticercosis blocking foramen of Monro bilaterally

    Science.gov (United States)

    Shah, Harshil Chimanlal; Jain, Kapil; Shah, Jaimin Kiran

    2016-01-01

    Neurocysticercosis (NCC) is a parasitic infestation of the central nervous system. NCC parasitic infestation can be misdiagnosed as hydatid cyst or intraventricular epidermoid cyst that can cause a diagnostic dilemma. A 23-year-old male patient presented with headache and vomiting for 3–4 days and giddiness for 4–5 days. Magnetic resonance imaging with contrast was suggestive of a rim-enhancing lesion at the level of the foramen of Monro. Endoscopic excision of the lesion was done, and the patient had relief of a headache and vomiting immediately after the procedure. He is being followed up regularly. Intraventricular NCC occluding both foramen of Monro is a rare entity. Complete endoscopic surgical excision followed by appropriate drug therapy should be given to achieve a cure. PMID:27057236

  17. Introduction: Lawrence Kohlberg as Mentor.

    Science.gov (United States)

    Boyd, Dwight

    1988-01-01

    Recognizes Lawrence Kohlberg as the foremost contributor to moral education during the twentieth century. Analyzes the mentor-student relationship and discusses Kohlberg's mentor relationship with his students. (KO)

  18. Lawrence Kohlberg: A Personal Tribute.

    Science.gov (United States)

    Mosher, Ralph

    1988-01-01

    Author reflects on his association with psychologist Lawrence Kohlberg and his collaboration with Kohlberg in a project funded by the Danforth Foundation for research and development in moral education. (Author/NB)

  19. Suicidal traits in Marilyn Monroe's Fragments: an LIWC analysis.

    Science.gov (United States)

    Fernández-Cabana, M; García-Caballero, A; Alves-Pérez, M T; García-García, M J; Mateos, R

    2013-01-01

    Linguistic inquiry and word count (LIWC), a computerized method for text analysis, is often used to examine suicide writings in order to characterize the quantitative linguistic features of suicidal texts. To analyze texts compiled in Marilyn Monroe's Fragments using LIWC, in order to explore the use of different linguistic categories in her narrative over the years. Selected texts were grouped into four periods of similar word count and processed with LIWC. Spearman's rank correlation was used to assess changes in language use across the documents over time. The Kruskal-Wallis test was applied to compare means between periods and for each of the 80 LIWC output scores. Significant differences (p Monroe approached the time of her death. The consistently elevated usage of first-person personal singular pronouns and the consistently diminished usage of first-person personal plural pronouns are in line with previous studies linking this pattern with a low level of social integration, which has been related to suicide according to different theories.

  20. LAWRENCE RADIATION LABORATORY COUNTING HANDBOOK

    Energy Technology Data Exchange (ETDEWEB)

    Group, Nuclear Instrumentation

    1966-10-01

    The Counting Handbook is a compilation of operational techniques and performance specifications on counting equipment in use at the Lawrence Radiation Laboratory, Berkeley. Counting notes have been written from the viewpoint of the user rather than that of the designer or maintenance man. The only maintenance instructions that have been included are those that can easily be performed by the experimenter to assure that the equipment is operating properly.

  1. Geophysical study of the Monroe-Red Hill geothermal system

    Energy Technology Data Exchange (ETDEWEB)

    Mase, C.W.; Chapman, D.S.; Ward, S.H.

    1978-10-01

    A detailed geophysical study consisting of heat flow, dipole-dipole resistivity, ground magnetics and gravity was conducted in the vicinity of Monroe, Utah to assess the resource potential of an identified hydrothermal system. The detailed study covered a 40 km/sup 2/ area along the Sevier fault near the Monroe-Red Hill hot springs. Fourteen 100m dipole-dipole resistivity profiles across the system were used to construct a first separation apparent resistivity contour map. The map effectively outlines the trace of the Sevier fault and reveals an elongate zone of low resistivity (< 10 ..cap omega..-m) associated with the hydrothermal system. Similar features are evident on the total magnetic intensity anomaly map. Gravity modeling across the system indicates that the Sevier fault is comprised of three or more nearly vertical en echelon faults. On the basis of geological mapping and surface geophysical surveys a series of eleven shallow boreholes (40 to 90m was drilled on two profiles across the system. Surface geothermal gradients vary from 240/sup 0/C km/sup -1/ to over 1000/sup 0/C km/sup -1/ along the profiles. Heat flow values vary smoothly from 550 mW m/sup -2/ to over 3000 mW m/sup -2/, a significant enhancement over background Basin and Range heat flow of 80 mW m/sup -2/. Heat budget calculations based on conductive heat loss and enthalpy of the discharge waters indicae a net power loss of 7.8 MW.

  2. Neuroendoscopic treatment of idiopathic occlusion of unilateral foramen of Monro presenting as chronic headache

    Directory of Open Access Journals (Sweden)

    Dhaval Shukla

    2016-01-01

    Full Text Available Asymmetric ventriculomegly due to idiopathic occlusion of the foramen of Monro is rare. Such patients present with clinical features of raised intracranial pressure (ICP. Presentation as chronic headache has not been previously described. In the absence of raised ICP, pursuing surgical treatment raises a clinical dilemma as the headache may be a primary headache with no improvement after surgery. A 21-year-old woman presented with chronic headache. She was found to have asymmetric ventriculomegaly due to the occlusion of the foramen of Monro. She underwent endoscopic septostomy and widening of the foramen of Monro. Her headache subsided after surgery. At 15 months of follow-up, she was free from headache without medications. Unilateral occlusion of the foramen of Monro can present with asymmetric ventriculomegaly resulting in chronic headache. Though the symptoms of raised ICP may not be present, still endoscopic relief of ventriculomegaly leads to cure of headache.

  3. Aerial Photography and Imagery, Ortho-Corrected - 2012 Digital Orthophotos - Monroe County

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This metadata describes the digital orthoimagery covering Monroe County, FL. This 1"=100' scale imagery is comprised of natural color orthoimagery with a GSD (Ground...

  4. Aerial Photography and Imagery, Ortho-Corrected - 2012 Digital Orthophotos - Monroe County

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This metadata describes the digital orthoimagery covering the area of the Everglades in Monroe County, FL as defined by the FL DOR. This 1"=100' scale imagery is...

  5. T. E. Lawrence: Theorist and Campaign Planner

    Science.gov (United States)

    1992-05-12

    honors in history, Lawrence’s curiosity lead him to the works of Carl von Clausewitz, Henri Jomini. Karl von Willisen, Rudolf von Caemmerer, Helmut von...113. M. J. Steiner . Inside Pan-Arabia. (Chicago: Herxiricks House, 1947), Chap 7. 114. T. E. Lawrence, "The Evolution of a Revolt," p65. 115. T. E...Unity. New York: Devin-Adair, 1958. Steiner . M. J. Inside Pan-Arabia. Chicago: Hendricks House. 1947. Thomas, Lowell. With Lawrence in Arabia. New York

  6. 75 FR 60495 - Notice of Intent To Rule on Request To Release Airport Property at Monroe Regional Airport...

    Science.gov (United States)

    2010-09-30

    ... Federal Aviation Administration Notice of Intent To Rule on Request To Release Airport Property at Monroe Regional Airport, Monroe, LA AGENCY: Federal Aviation Administration (FAA) DOT. ACTION: Request for Public... Regional Airport under the provisions of Title 49, U.S.C. Section 47153(c). DATES: Comments must...

  7. A Robbins-Monro procedure for estimation in semiparametric regression models

    CERN Document Server

    Bercu, Bernard

    2011-01-01

    This paper is devoted to the parametric estimation of a shift together with the nonparametric estimation of a regression function in a semiparametric regression model. We implement a Robbins-Monro procedure very efficient and easy to handle. On the one hand, we propose a stochastic algorithm similar to that of Robbins-Monro in order to estimate the shift parameter. A preliminary evaluation of the regression function is not necessary for estimating the shift parameter. On the other hand, we make use of a recursive Nadaraya-Watson estimator for the estimation of the regression function. This kernel estimator takes in account the previous estimation of the shift parameter. We establish the almost sure convergence for both Robbins-Monro and Nadaraya-Watson estimators. The asymptotic normality of our estimates is also provided.

  8. Mr Lawrence'ilt best-of

    Index Scriptorium Estoniae

    2007-01-01

    Renee Meriste ja muusikaettevõtte Eesti Artistide Agentuuri poolt välja antavate heliplaatide seas ka 12. dets. ilmuvast rokkansambli Mr. Lawrence kogumikalbumist "Greatest Hits", heliplaatide sarjast "Eesti Rock Antoloogia"

  9. Lawrence Kohlberg: As A Moral Educator

    OpenAIRE

    Çinemre, Arş. Gör. Semra

    2013-01-01

    Lawrence Kohlberg: As A Moral Educator Lawrence Kohlberg (1927-1987) is a scholar who has comprehensive knowledge of many fields especially philosophy, psychology, sociology, anthropology and education, and who is known clearly for cognitive moral development theory. Having looked at the works in national and international scale which are done on Kohlberg, it is seen generally that his moral development theory has been emphasized and that his moral educational works have been neglected. In fa...

  10. On the Historical Significance and Structure of Monroe Beardsley's "Aesthetics": An Appreciation

    Science.gov (United States)

    Carroll, Noel

    2010-01-01

    Monroe C. Beardsley's "Aesthetics: Problems in the Philosophy of Criticism," published in 1958 by Harcourt, Brace and World Inc., was a watershed event in the history of analytic aesthetics--a climax of sorts with respect to what preceded it and, at the same time, the opening of a new, more intricately developed and defended research program in…

  11. 33 CFR 334.350 - Chesapeake Bay off Fort Monroe, Va.; firing range danger zone.

    Science.gov (United States)

    2010-07-01

    ..., Va.; firing range danger zone. 334.350 Section 334.350 Navigation and Navigable Waters CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE DANGER ZONE AND RESTRICTED AREA REGULATIONS § 334.350 Chesapeake Bay off Fort Monroe, Va.; firing range danger zone. (a) The danger zone. All of...

  12. Endoscopic resection of cavernoma of foramen of Monro in a patient with familial multiple cavernomatosis.

    Science.gov (United States)

    Prat, Ricardo; Galeano, Inmaculada

    2008-09-01

    Intraventricular cavernomas are extremely infrequent and only 11 cases of cavernous hemangioma to occur at the foramen of Monro have been reported in the literature. This 56 years old patient was admitted with progressive and intractable headache of 10 days of evolution. He was known to suffer familial multiple cavernomatosis. Magnetic resonance imaging (MRI), revealed obstructive hydrocephalus due to a cavernoma located in the area of the left foramen of Monro. Under neuronavigation guidance, complete endoscopic resection of the cavernoma was performed and normal ventricular size achieved. The patient experienced transient recent memory loss that resolved within a month after surgery. In the literature attempted endoscopic resection is reported to be abandoned due to bleeding and ineffectiveness of piecemeal endoscopic resection. In this case, the multiplicity of the lesions made it advisable to resect the lesion endoscopically, to avoid an open procedure in a patient with multiple potentially surgical lesions. Endoscopic resection was uneventful with easy control of bleeding with irrigation, suction, and bipolar coagulation despite dense vascular appearance of the lesion. During the procedure, precise visualization of the vascular structures around the foramen of Monro allowed complete resection with satisfactory control of the instruments. To the best of the authors' knowledge, this is the first published cavernoma of foramen of Monro successfully resected using an endoscopic approach.

  13. High-Dimensional Exploratory Item Factor Analysis by a Metropolis-Hastings Robbins-Monro Algorithm

    Science.gov (United States)

    Cai, Li

    2010-01-01

    A Metropolis-Hastings Robbins-Monro (MH-RM) algorithm for high-dimensional maximum marginal likelihood exploratory item factor analysis is proposed. The sequence of estimates from the MH-RM algorithm converges with probability one to the maximum likelihood solution. Details on the computer implementation of this algorithm are provided. The…

  14. Metropolis-Hastings Robbins-Monro Algorithm for Confirmatory Item Factor Analysis

    Science.gov (United States)

    Cai, Li

    2010-01-01

    Item factor analysis (IFA), already well established in educational measurement, is increasingly applied to psychological measurement in research settings. However, high-dimensional confirmatory IFA remains a numerical challenge. The current research extends the Metropolis-Hastings Robbins-Monro (MH-RM) algorithm, initially proposed for…

  15. Contrasting Models of Posttraumatic Stress Disorder: Reply to Monroe and Mineka (2008)

    Science.gov (United States)

    Berntsen, Dorthe; Rubin, David C.; Bohni, Malene Klindt

    2008-01-01

    The authors address the 4 main points in S. M. Monroe and S. Mineka's comment. First, the authors show that the Diagnostic and Statistical Manual of Mental Disorders (4th ed., text rev.; American Psychiatric Association, 2000) posttraumatic stress disorder (PTSD) diagnosis includes an etiology and that it is based on a theoretical model with a…

  16. High-Dimensional Exploratory Item Factor Analysis by a Metropolis-Hastings Robbins-Monro Algorithm

    Science.gov (United States)

    Cai, Li

    2010-01-01

    A Metropolis-Hastings Robbins-Monro (MH-RM) algorithm for high-dimensional maximum marginal likelihood exploratory item factor analysis is proposed. The sequence of estimates from the MH-RM algorithm converges with probability one to the maximum likelihood solution. Details on the computer implementation of this algorithm are provided. The…

  17. Metropolis-Hastings Robbins-Monro Algorithm for Confirmatory Item Factor Analysis

    Science.gov (United States)

    Cai, Li

    2010-01-01

    Item factor analysis (IFA), already well established in educational measurement, is increasingly applied to psychological measurement in research settings. However, high-dimensional confirmatory IFA remains a numerical challenge. The current research extends the Metropolis-Hastings Robbins-Monro (MH-RM) algorithm, initially proposed for…

  18. James Monroe High School Proyecto Nuevos Horizontes, 1986-1987. OEA Evaluation Report.

    Science.gov (United States)

    Martinez, Ana L.; And Others

    In its second year of Title VII funding, James Monroe High Schools's Proyecto Nuevos Horizontes (Project New Horizons) served 344 limited-English-speaking recent arrivals from Latin America and the Caribbean, in grades 9 through 12. The program has built on the strengths of the high school's extensive computer-assisted instruction (CAI) program,…

  19. Nuevos Horizontes, James Monroe High School, 1987-1988. Evaluation Section Report. OREA Report.

    Science.gov (United States)

    Berney, Tomi D.; Lista, Carlos

    Proyecto Nuevos Horizontes (Project New Horizons) at James Monroe High School (New York City) served 328 students of limited English proficiency (LEP) in grades 9-12 during the final year of a 3-year funding cycle. The project's purpose was to build on the strengths of the school's extensive computer-assisted instructional program in order to…

  20. James Monroe High School Proyecto Nuevos Horizontes, 1985-1986. OEA Evaluation Report.

    Science.gov (United States)

    New York City Board of Education, Brooklyn. Office of Educational Assessment.

    Proyecto Nuevos Horizontes, a 3-year Title VII-funded bilingual education program, serves 287 Spanish speaking students at James Monroe High School (Bronx, New York). This report evaluates the project's first year of operation, 1985-86. The report contains an introduction describing the school and project goals; information on student…

  1. 78 FR 17468 - Alabama Railroad Co.-Abandonment Exemption-in Monroe County, AL

    Science.gov (United States)

    2013-03-21

    ... Abandonments to abandon approximately 7.42 miles of rail line between milepost 655.20 (east of Route 21 at Tunnel Springs) and milepost 662.62 (west of Main Street in Beatrice), in Monroe County, Ala. The line traverses United States Postal Service Zip Codes 36425 and 36471. ALAB has certified that: (1) No...

  2. Endoscopic management of large multicompartmental intraventricular arachnoid cyst extending from foramen magnum to foramen of Monro

    Directory of Open Access Journals (Sweden)

    Yadav Y

    2010-01-01

    Full Text Available The treatment options for symptomatic arachnoid cysts are shunting, open craniotomy, and endoscopic fenestration. Endoscopic fenestration of large arachnoid cyst is safe and effective. Postoperative subdural hematoma and intraparenchymal hemorrhage can be avoided by endoscopic fenestration. This technique has the additional advantage of identifying and treating ventricular abnormalities, such as foramen of Monro stenosis and cerebral aqueduct occlusion. This report describes endoscopic dual fenestration in a child with large multicompartmental intraventricular arachnoid cyst extending from foramen magnum to foramen of Monro. The child presented with difficulty to hold the neck in upright position, irritability, vomiting, and large head. Follow-up postoperative magnetic resonance imaging at 3 months showed a significant reduction in size of the cyst. Clinically, the patient showed a gradual improvement at 10 months follow-up. Probably this is the first report of this nature.

  3. Uma política para o continente - reinterpretando a Doutrina Monroe

    Directory of Open Access Journals (Sweden)

    Carlos Gustavo Poggio Teixeira

    2014-12-01

    Full Text Available A declaração de novembro de 2013 do Secretário de Estado dos Estados Unidos, John Kerry, de que "a era da Doutrina Monroe terminou" é uma excelente oportunidade para rediscutir essa que é considerada um dos princípios cardeais da política externa norte-americana para a América Latina desde a independência dos Estados latino-americanos. O presente artigo pretende jogar luz em um aspecto frequentemente menosprezado pelas análises acerca da Doutrina Monroe, qual seja, o fato de que tal doutrina, longe de ser uma política concreta para todo o continente, tinha duas facetas bastante distintas tanto do ponto vista retórico como político. Através do exame principalmente de bibliografia produzida nos Estados Unidos, em especial daquelas que tiveram acesso a fontes primárias, o artigo demonstra que a Doutrina Monroe tinha um caráter claramente caribenho - e não latino-americano - a partir do reconhecimento, por parte tanto dos formuladores de política dos Estados Unidos como dos sul-americanos, de que a América do Sul era um sistema separado da América do Norte. Assim, o artigo defende que ao sul do Panamá, a Doutrina Monroe adquiriu um aspecto consideravelmente mais "multilateral", o que foi evidenciado até mesmo pelo seu famoso Corolário Roosevelt.

  4. Controversial neuroendoscopic Monro foraminoplasty in the management of isolated lateral ventricle in an adult

    Directory of Open Access Journals (Sweden)

    Max O. Krucoff

    2015-06-01

    Full Text Available This report describes the clinical presentation, differential diagnosis, imaging characteristics, and current literature regarding the minimally invasive neuroendoscopic treatment of a rare condition: idiopathic unilateral hydrocephalus presenting late in adulthood. This paper also addresses the current controversy regarding septum pellucidotomy and/or Monro foraminoplasty as mono- or dual therapies for UH. A 63 year old African American female presented with headaches, blurry vision, and gait instability for six months. She was referred for neurosurgical consultation after an MRI demonstrated enlargement of the right lateral ventricle with left septal deviation. The patient underwent endoscopic transventricular fenestration of an idiopathic membrane occluding the foramen of Monro, as well as foraminoplasty of the foramen of Monro and septum pellucidotomy. Postoperatively the patient had transient difficulty with short term recall that improved rapidly, and she was discharged home on postoperative day 2. Follow-up one month later demonstrated complete resolution of her headache, blurry vision, and imbalance, as well as continued improvement of her memory. At six months, she had durable resolution of hydrocephalus and no short term memory complaints.

  5. Lawrence Livermore National Laboratory Environmental Report 2014

    Energy Technology Data Exchange (ETDEWEB)

    Jones, H. E. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Bertoldo, N. A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Blake, R. G. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Buscheck, W. M. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Byrne, J. G. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Cerruti, S. J. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Bish, C. B. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Fratanduono, M. E. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Grayson, A. R. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); MacQueen, D. H. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Montemayor, W. E. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Ottaway, H. L. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Paterson, L. E. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Revelli, M. A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Rosene, C. A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Swanson, K. A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Terrill, A. A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Wegrecki, A. M. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Wilson, K. R. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Woollett, J. S. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2015-09-29

    The purposes of the Lawrence Livermore National Laboratory Environmental Report 2014 are to record Lawrence Livermore National Laboratory’s (LLNL’s) compliance with environmental standards and requirements, describe LLNL’s environmental protection and remediation programs, and present the results of environmental monitoring at the two LLNL sites—the Livermore Site and Site 300. The report is prepared for the U.S. Department of Energy (DOE) by LLNL’s Environmental Functional Area. Submittal of the report satisfies requirements under DOE Order 231.1B, “Environment, Safety and Health Reporting,” and DOE Order 458.1, “Radiation Protection of the Public and Environment.”

  6. Lawrence Livermore National Laboratory Environmental Report 2015

    Energy Technology Data Exchange (ETDEWEB)

    Rosene, C. A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Jones, H. E. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2016-09-22

    The purposes of the Lawrence Livermore National Laboratory Environmental Report 2015 are to record Lawrence Livermore National Laboratory’s (LLNL’s) compliance with environmental standards and requirements, describe LLNL’s environmental protection and remediation programs, and present the results of environmental monitoring at the two LLNL sites—the Livermore Site and Site 300. The report is prepared for the U.S. Department of Energy (DOE) by LLNL’s Environmental Functional Area. Submittal of the report satisfies requirements under DOE Order 231.1B, “Environment, Safety and Health Reporting,” and DOE Order 458.1, “Radiation Protection of the Public and Environment.”

  7. Lawrence Berkeley Laboratory 1993 Site Environmental Report

    Energy Technology Data Exchange (ETDEWEB)

    1994-05-01

    This annual Site Environmental Report summarizes Lawrence Berkeley Laboratory`s (LBL`s) environmental activities in calendar year (CY) 1993. The purpose of this report is to characterize site environmental management performance, confirm compliance status with environmental standards and requirements, and highlight significant programs and efforts. Its format and content are consistent with the requirements of the US Department of Energy (DOE) Order 5400.1, General Environmental Protection Program.

  8. Life sciences: Lawrence Berkeley Laboratory, 1988

    Energy Technology Data Exchange (ETDEWEB)

    1989-07-01

    Life Sciences Research at LBL has both a long history and a new visibility. The physics technologies pioneered in the days of Ernest O. Lawrence found almost immediate application in the medical research conducted by Ernest's brother, John Lawrence. And the tradition of nuclear medicine continues today, largely uninterrupted for more than 50 years. Until recently, though, life sciences research has been a secondary force at the Lawrence Berkeley Laboratory (LBL). Today, a true multi-program laboratory has emerged, in which the life sciences participate as a full partner. The LBL Human Genome Center is a contribution to the growing international effort to map the human genome. Its achievements represent LBL divisions, including Engineering, Materials and Chemical Sciences, and Information and Computing Sciences, along with Cell and Molecular Biology and Chemical Biodynamics. The Advanced Light Source Life Sciences Center will comprise not only beamlines and experimental end stations, but also supporting laboratories and office space for scientists from across the US. This effort reflects a confluence of scientific disciplines --- this time represented by individuals from the life sciences divisions and by engineers and physicists associated with the Advanced Light Source project. And finally, this report itself, the first summarizing the efforts of all four life sciences divisions, suggests a new spirit of cooperation. 30 figs.

  9. Obituary: Lawrence Hugh Aller, 1913-2003

    Science.gov (United States)

    Kaler, James B.

    2003-12-01

    The announcement still lies in my inbox: ``Lawrence Aller died last Sunday." On 16 March 2003, one of the world's fine astronomers passed away at the age of 89, leaving behind a legacy that will ripple as long as there are students of the celestial science, one that incorporated observation, theory, education, care, decency, and kindness. Lawrence was born in the humblest of conditions in Tacoma, Washington, on 24 September 1913. His mother, Lella (Belle) Allen, was a homemaker and his father Leslie Aller, was an occassional printer and gold prospector who thought that the use of the mind was a waste of time. With fierce persistence and dedication, Lawrence pulled off a feat that would probably not be possible now: getting into college without having finished high school, the result of being dragged to work in his father's primitive gold mining camp. His interest, sparked by leaflets from the Astronomical Society of the Pacific and by Russell, Dugan, and Stewart's venerable textbook, led him to a correspondence, and finally a meeting, with Donald Menzel of Harvard, who persuaded the admissions director of the University of California at Berkeley to admit him in 1932. From there, Lawrence went on to graduate school at Harvard and the Harvard Society of Fellows, where he studied with Menzel and developed his interest in stellar and nebular astronomy. After working in the War effort, he made his professorial debut at Indiana University, where he stayed until 1948 before leaving for the University of Michigan. Residing there for the next 14 years, he established his research reputation and helped develop the Michigan graduate program. In 1962, the opportunity arose to return to California, to UCLA, where he again was instrumental in founding a PhD program. There he stayed, through his retirement in 1984, doing research right up to the end. Eight other schools received him as visiting professor. Lawrence knew that to make inroads into astronomy, he needed to apply

  10. Lawrence Berkeley Laboratory 1994 site environmental report

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-05-01

    The 1994 Site Environmental Report summarizes environmental activities at Lawrence Berkeley Laboratory (LBL) for the calendar year (CY) 1994. The report strives to present environmental data in a manner that characterizes the performance and compliance status of the Laboratory`s environmental management programs when measured against regulatory standards and DOE requirements. The report also discusses significant highlight and planning efforts of these programs. The format and content of the report are consistent with the requirements of the U.S. Department of Energy (DOE) Order 5400.1, General Environmental Protection Program.

  11. Pressure safety program Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Borzileri, C.; Traini, M.

    1992-10-01

    The Lawrence Livermore National Laboratory (LLNL) is a Research and Development facility. Programs include research in: nuclear weapons, energy, environmental, biomedical, and other DOE funded programs. LLNL is managed by the University of California for the Department of Energy. Many research and development programs require the use of pressurized fluid systems. In the early 1960`s, courses were developed to train personnel to safely work with pressurized systems. These courses served as a foundation for the Pressure Safety Program. The Pressure Safety Program is administered by the Pressure Safety Manager through the Hazards Control Department, and responsibilities include: (1) Pressure Safety course development and training, (2) Equipment documentation, tracking and inspections/retests, (3) Formal and informal review of pressure systems. The program uses accepted codes and standards and closely follows the DOE Pressure Safety Guidelines Manual. This manual was developed for DOE by Lawrence Livermore National Laboratory. The DOE Pressure Safety Guidelines Manual defines five (5) basic elements which constitute this Pressure Safety Program. These elements are: (1) A Pressure Safety Manual, (2) A Safety Committee, (3) Personnel who are trained and qualified, (4) Documentation and accountability for each pressure vessel or system, (5) Control of the selection and the use of high pressure hardware.

  12. Monro-Kellie 2.0: The dynamic vascular and venous pathophysiological components of intracranial pressure

    Science.gov (United States)

    2016-01-01

    For 200 years, the ‘closed box’ analogy of intracranial pressure (ICP) has underpinned neurosurgery and neuro-critical care. Cushing conceptualised the Monro-Kellie doctrine stating that a change in blood, brain or CSF volume resulted in reciprocal changes in one or both of the other two. When not possible, attempts to increase a volume further increase ICP. On this doctrine’s “truth or relative untruth” depends many of the critical procedures in the surgery of the central nervous system. However, each volume component may not deserve the equal weighting this static concept implies. The slow production of CSF (0.35 ml/min) is dwarfed by the dynamic blood in and outflow (∼700 ml/min). Neuro-critical care practice focusing on arterial and ICP regulation has been questioned. Failure of venous efferent flow to precisely match arterial afferent flow will yield immediate and dramatic changes in intracranial blood volume and pressure. Interpreting ICP without interrogating its core drivers may be misleading. Multiple clinical conditions and the cerebral effects of altitude and microgravity relate to imbalances in this dynamic rather than ICP per se. This article reviews the Monro-Kellie doctrine, categorises venous outflow limitation conditions, relates physiological mechanisms to clinical conditions and suggests specific management options. PMID:27174995

  13. Autogrammid, oma aja märk / Mike Lawrence

    Index Scriptorium Estoniae

    Lawrence, Mike

    2004-01-01

    Autogrammide kogumisest, nende ehtsusest, sportlastele kuulunud esemete kollektsioneerimisest. Lisatud: Kollektsionääride maiuspalu. Autor Mike Lawrence on oksjonifirma Bonhams/Brooks konsultant, ajakirjanik

  14. Autogrammid, oma aja märk / Mike Lawrence

    Index Scriptorium Estoniae

    Lawrence, Mike

    2004-01-01

    Autogrammide kogumisest, nende ehtsusest, sportlastele kuulunud esemete kollektsioneerimisest. Lisatud: Kollektsionääride maiuspalu. Autor Mike Lawrence on oksjonifirma Bonhams/Brooks konsultant, ajakirjanik

  15. miRSeqNovel

    DEFF Research Database (Denmark)

    Qian, Kui; Auvinen, Eeva; Greco, Dario

    2012-01-01

    We present miRSeqNovel, an R based workflow for miRNA sequencing data analysis. miRSeqNovel can process both colorspace (SOLiD) and basespace (Illumina/Solexa) data by different mapping algorithms. It finds differentially expressed miRNAs and gives conservative prediction of novel miRNA candidates...... with customized parameters. miRSeqNovel is freely available at http://sourceforge.net/projects/mirseq/files....

  16. Estimation of Contextual Effects through Nonlinear Multilevel Latent Variable Modeling with a Metropolis-Hastings Robbins-Monro Algorithm

    Science.gov (United States)

    Yang, Ji Seung; Cai, Li

    2014-01-01

    The main purpose of this study is to improve estimation efficiency in obtaining maximum marginal likelihood estimates of contextual effects in the framework of nonlinear multilevel latent variable model by adopting the Metropolis-Hastings Robbins-Monro algorithm (MH-RM). Results indicate that the MH-RM algorithm can produce estimates and standard…

  17. Estimation of Contextual Effects through Nonlinear Multilevel Latent Variable Modeling with a Metropolis-Hastings Robbins-Monro Algorithm

    Science.gov (United States)

    Yang, Ji Seung; Cai, Li

    2014-01-01

    The main purpose of this study is to improve estimation efficiency in obtaining maximum marginal likelihood estimates of contextual effects in the framework of nonlinear multilevel latent variable model by adopting the Metropolis-Hastings Robbins-Monro algorithm (MH-RM). Results indicate that the MH-RM algorithm can produce estimates and standard…

  18. 78 FR 57682 - Norfolk Southern Railway Company-Discontinuance of Service Exemption-in Monroe County, Mich.

    Science.gov (United States)

    2013-09-19

    ... service over an approximately 1.7-mile line of railroad extending between milepost XV 0.0 and milepost XV 1.7, in Monroe County, Mich. (the Line). The Line traverses United States Postal Service Zip Code 48161. NSR has certified that: (1) No local traffic has moved over the Line for at least two years;...

  19. miRMaid

    DEFF Research Database (Denmark)

    Jacobsen, Anders; Krogh, Anders; Kauppinen, Sakari;

    2010-01-01

    here as miRMaid, with the goal of integrating miRNA data resources in a uniform web service interface that can be accessed and queried by researchers and, most importantly, by computers. miRMaid is built around data from miRBase and is designed to follow the official miRBase data releases. It exposes...... application. The software framework is freely available under the LGPL open source license for academic and commercial use. CONCLUSION: miRMaid is an intuitive and modular software platform designed to unify miRBase and independent miRNA data resources. It enables miRNA researchers to computationally address...... complex questions involving the multitude of miRNA data resources. Furthermore, miRMaid constitutes a basic framework for further programming in which microRNA-interested bioinformaticians can readily develop their own tools and data sources....

  20. Geologic map of Saint Lawrence Island, Alaska

    Science.gov (United States)

    Patton, William W.; Wilson, Frederic H.; Taylor, Theresa A.

    2011-01-01

    Saint Lawrence Island is located in the northern Bering Sea, 190 km southwest of the tip of the Seward Peninsula, Alaska, and 75 km southeast of the Chukotsk Peninsula, Russia (see index map, map sheet). It lies on a broad, shallow-water continental shelf that extends from western Alaska to northeastern Russia. The island is situated on a northwest-trending structural uplift exposing rocks as old as Paleozoic above sea level. The submerged shelf between the Seward Peninsula and Saint Lawrence Island is covered mainly with Cenozoic deposits (Dundo and Egiazarov, 1982). Northeast of the island, the shelf is underlain by a large structural depression, the Norton Basin, which contains as much as 6.5 km of Cenozoic strata (Grim and McManus, 1970; Fisher and others, 1982). Sparse test-well data indicate that the Cenozoic strata are underlain by Paleozoic and Proterozoic rocks, similar to those exposed on the Seward Peninsula (Turner and others, 1983). Saint Lawrence Island is 160 km long in an east-west direction and from 15 km to 55 km wide in a north-south direction. The east end of the island consists largely of a wave-cut platform, which has been elevated as much as 30 m above sea level. Isolated upland areas composed largely of granitic plutons rise as much as 550 m above the wave-cut platform. The central part of the island is dominated by the Kookooligit Mountains, a large Quaternary shield volcano that extends over an area of 850 km2 and rises to an elevation of 630 m. The west end of the island is composed of the Poovoot Range, a group of barren, rubble-covered hills as high as 450 m that extend from Boxer Bay on the southwest coast to Taphook Mountain on the north coast. The Poovoot Range is flanked on the southeast by the Putgut Plateau, a nearly flat, lake-dotted plain that stands 30?60 m above sea level. The west end of the island is marked by uplands underlain by the Sevuokuk pluton (unit Kg), a long narrow granite body that extends from Gambell on the

  1. Saint Lawrence Seaway Additional Locks Study. Appendices.

    Science.gov (United States)

    1982-07-01

    Lo.e. a 03.334 2 7.490 2 2 64.702 1 .093.700 21.160.800 21.231.000 21.385.000 2 1.1.00 Wet. O...u.7 t.. Wit ?1 100 3 .573.M9 1 $.375.231 7.003.000 2...Benefits - Low Traffic B-63 Forecast (80 Percent Utilization) B17 Summary of Rate Savings Benefits - High Traffic B-65 Forecast B18 Future Fleet...Projection B-37 B16 St. Lawrence River - General Cargo Projection B-38 B16a Conceptual Block Diagram for Lock Capacity Model B-41 B17 Class 10 and 11

  2. Lawrence Berkeley Laboratory Affirmative Action Program. Revised

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-06-01

    The Lawrence Berkeley Laboratory`s Affirmative Action Program (AAP) serves as a working document that describes current policies, practices, and results in the area of affirmative action. It represents the Laboratory`s framework for an affirmative approach to increasing the representation of people of color and women in segments of our work force where they have been underrepresented and taking action to increase the employment of persons with disabilities and special disabled and Vietnam era veterans. The AAP describes the hierarchy of responsibility for Laboratory affirmative action, the mechanisms that exist for full Laboratory participation in the AAP, the policies and procedures governing recruitment at all levels, the Laboratory`s plan for monitoring, reporting, and evaluating affirmative action progress, and a description of special affirmative action programs and plans the Laboratory has used and will use in its efforts to increase the representation and retention of groups historically underrepresented in our work force.

  3. Adaptive Optics at Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Gavel, D T

    2003-03-10

    Adaptive optics enables high resolution imaging through the atmospheric by correcting for the turbulent air's aberrations to the light waves passing through it. The Lawrence Livermore National Laboratory for a number of years has been at the forefront of applying adaptive optics technology to astronomy on the world's largest astronomical telescopes, in particular at the Keck 10-meter telescope on Mauna Kea, Hawaii. The technology includes the development of high-speed electrically driven deformable mirrors, high-speed low-noise CCD sensors, and real-time wavefront reconstruction and control hardware. Adaptive optics finds applications in many other areas where light beams pass through aberrating media and must be corrected to maintain diffraction-limited performance. We describe systems and results in astronomy, medicine (vision science), and horizontal path imaging, all active programs in our group.

  4. Euroopal tuleb leida vastus Vene Monroe-doktriinile / Lennart Meri ; intervjueerinud Olaf Ihlan, Andreas Lorentz

    Index Scriptorium Estoniae

    Meri, Lennart, 1929-2006

    1993-01-01

    Eesti Vabariigi president L. Meri Baltikumist ja suurvene šovinismist. Intervjuu ajakirjale "Der Spiegel", nr. 44, 1. nov. 1993, lk. 200, 202-203, 206, pealkiri: "Dann wird Europa verbluten" : Estlands Präsident Lennart Meri über das Baltikum und den grossrussischen Chauvinismus. Ilmunud ka: Õhtuleht, 8. nov., lk. 5, pealkiri: "Siis voolab Euroopas veri" : Eesti president Lennart Meri Baltikumist ja suurvene šovinismist ; Rahva Hääl, 9. nov., lk., 3 ; Eesti Rada (Köln, 1993) nr. 6, lk. 3, pealkiri: President Lennart Meri intervjuu "Spiegelile" / refereerinud ja tsiteerinud J. P ; Eesti Päevaleht (Stockholm), 24. nov., lk. 2 : ill., pealkiri: President Lennart Meri : "Venemaa laiendab oma huvisfääri" ; Vaba Eestlane (Toronto), 9. dets., lk. 3, 11, pealkiri: "Euroopal tuleb leida vastus Vene - Monroe doktriinile" ; Meie Kodu, 15. dets., lk. 6, pealkiri: Eesti Vabariigi President Lennart Meri Baltikumist ja suurvene šovinismist

  5. Neuroendoscopic surgery for unilateral hydrocephalus due to inflammatory obstruction of the Monro foramen

    Directory of Open Access Journals (Sweden)

    Francisco A. Vaz-Guimarães Filho

    2011-04-01

    Full Text Available OBJECTIVE: Unilateral hydrocephalus (UH is characterized by enlargement of just one lateral ventricle. In this paper, the authors will demonstrate their experiences in the neuroendoscopic management of this uncommon type of hydrocephalus. METHOD: The authors retrospectively reviewed a serie of almost 800 neuroendoscopic procedures performed from September 1995 to July 2010 and selected seven adult patients with UH. Clinical and radiological charts were reviewed and analyzed. RESULTS: Six patients had intraventricular neurocysticercosis and one patient had congenital stenosis of the foramen of Monro. Headaches were the most common symptom. A septostomy restored cerebrospinal fluid circulation. During follow-up period (65.5 months, range 3-109 no patient has presented clinical recurrence as well as no severe complications have been observed. CONCLUSION: UH is a rare condition. A successful treatment can be accomplished through a neuroendoscopic approach avoiding the use of ventricular shunts.

  6. Hydrogeology and simulation of regional ground-water-level declines in Monroe County, Michigan

    Science.gov (United States)

    Reeves, Howard W.; Wright, Kirsten V.; Nicholas, J.R.

    2004-01-01

    Observed ground-water-level declines from 1991 to 2003 in northern Monroe County, Michigan, are consistent with increased ground-water demands in the region. In 1991, the estimated ground-water use in the county was 20 million gallons per day, and 80 percent of this total was from quarry dewatering. In 2001, the estimated ground-water use in the county was 30 million gallons per day, and 75 percent of this total was from quarry dewatering. Prior to approximately 1990, the ground-water demands were met by capturing natural discharge from the area and by inducing leakage through glacial deposits that cover the bedrock aquifer. Increased ground-water demand after 1990 led to declines in ground-water level as the system moves toward a new steady-state. Much of the available natural discharge from the bedrock aquifer had been captured by the 1991 conditions, and the response to additional withdrawals resulted in the observed widespread decline in water levels. The causes of the observed declines were explored through the use of a regional ground-water-flow model. The model area includes portions of Lenawee, Monroe, Washtenaw, and Wayne Counties in Michigan, and portions of Fulton, Henry, and Lucas Counties in Ohio. Factors, including lowered water-table elevations because of below average precipitation during the time period (1991 - 2001) and reduction in water supply to the bedrock aquifer because of land-use changes, were found to affect the regional system, but these factors did not explain the regional decline. Potential ground-water capture for the bedrock aquifer in Monroe County is limited by the low hydraulic conductivity of the overlying glacial deposits and shales and the presence of dense saline water within the bedrock as it dips into the Michigan Basin to the west and north of the county. Hydrogeologic features of the bedrock and the overlying glacial deposits were included in the model design. An important step of characterizing the bedrock aquifer was the

  7. Euroopal tuleb leida vastus Vene Monroe-doktriinile / Lennart Meri ; intervjueerinud Olaf Ihlan, Andreas Lorentz

    Index Scriptorium Estoniae

    Meri, Lennart, 1929-2006

    1993-01-01

    Eesti Vabariigi president L. Meri Baltikumist ja suurvene šovinismist. Intervjuu ajakirjale "Der Spiegel", nr. 44, 1. nov. 1993, lk. 200, 202-203, 206, pealkiri: "Dann wird Europa verbluten" : Estlands Präsident Lennart Meri über das Baltikum und den grossrussischen Chauvinismus. Ilmunud ka: Õhtuleht, 8. nov., lk. 5, pealkiri: "Siis voolab Euroopas veri" : Eesti president Lennart Meri Baltikumist ja suurvene šovinismist ; Rahva Hääl, 9. nov., lk., 3 ; Eesti Rada (Köln, 1993) nr. 6, lk. 3, pealkiri: President Lennart Meri intervjuu "Spiegelile" / refereerinud ja tsiteerinud J. P ; Eesti Päevaleht (Stockholm), 24. nov., lk. 2 : ill., pealkiri: President Lennart Meri : "Venemaa laiendab oma huvisfääri" ; Vaba Eestlane (Toronto), 9. dets., lk. 3, 11, pealkiri: "Euroopal tuleb leida vastus Vene - Monroe doktriinile" ; Meie Kodu, 15. dets., lk. 6, pealkiri: Eesti Vabariigi President Lennart Meri Baltikumist ja suurvene šovinismist

  8. A Robbins-Monro procedure for a class of models of deformation

    CERN Document Server

    Fraysse, Philippe

    2012-01-01

    The paper deals with the statistical analysis of several data sets associated with shape invariant models with different translation, height and scaling parameters. We propose to estimate these parameters together with the common shape function. Our approach extends the recent work of Bercu and Fraysse to multivariate shape invariant models. We propose a very efficient Robbins-Monro procedure for the estimation of the translation parameters and we use these estimates in order to evaluate scale parameters. The main pattern is estimated by a weighted Nadaraya-Watson estimator. We provide almost sure convergence and asymptotic normality for all estimators. Finally, we illustrate the convergence of our estimation procedure on simulated data as well as on real ECG data.

  9. Lawrence Livermore National Laboratory Environmental Report 2010

    Energy Technology Data Exchange (ETDEWEB)

    Jones, H E; Bertoldo, N A; Campbell, C G; Cerruti, S J; Coty, J D; Dibley, V R; Doman, J L; Grayson, A R; MacQueen, D H; Wegrecki, A M; Armstrong, D H; Brigdon, S L; Heidecker, K R; Hollister, R K; Khan, H N; Lee, G S; Nelson, J C; Paterson, L E; Salvo, V J; Schwartz, W W; Terusaki, S H; Wilson, K R; Woods, J M; Yimbo, P O; Gallegos, G M; Terrill, A A; Revelli, M A; Rosene, C A; Blake, R G; Woollett, J S; Kumamoto, G

    2011-09-14

    The purposes of the Lawrence Livermore National Laboratory Environmental Report 2010 are to record Lawrence Livermore National Laboratory's (LLNL's) compliance with environmental standards and requirements, describe LLNL's environmental protection and remediation programs, and present the results of environmental monitoring at the two LLNL sites - the Livermore site and Site 300. The report is prepared for the U.S. Department of Energy (DOE) by LLNL's Environmental Protection Department. Submittal of the report satisfies requirements under DOE Order 231.1A, Environmental Safety and Health Reporting, and DOE Order 5400.5, Radiation Protection of the Public and Environment. The report is distributed electronically and is available at https://saer.llnl.gov/, the website for the LLNL annual environmental report. Previous LLNL annual environmental reports beginning in 1994 are also on the website. Some references in the electronic report text are underlined, which indicates that they are clickable links. Clicking on one of these links will open the related document, data workbook, or website that it refers to. The report begins with an executive summary, which provides the purpose of the report and an overview of LLNL's compliance and monitoring results. The first three chapters provide background information: Chapter 1 is an overview of the location, meteorology, and hydrogeology of the two LLNL sites; Chapter 2 is a summary of LLNL's compliance with environmental regulations; and Chapter 3 is a description of LLNL's environmental programs with an emphasis on the Environmental Management System including pollution prevention. The majority of the report covers LLNL's environmental monitoring programs and monitoring data for 2010: effluent and ambient air (Chapter 4); waters, including wastewater, storm water runoff, surface water, rain, and groundwater (Chapter 5); and terrestrial, including soil, sediment, vegetation, foodstuff

  10. Lawrence Livermore National Laboratory Environmental Report 2012

    Energy Technology Data Exchange (ETDEWEB)

    Jones, Henry E. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Armstrong, Dave [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Blake, Rick G. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Bertoldo, Nicholas A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Cerruti, Steven J. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Fish, Craig [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Dibley, Valerie R. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Doman, Jennifer L. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Grayson, Allen R. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Heidecker, Kelly R. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Hollister, Rod K. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Kumamoto, Gene [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); MacQueen, Donald H. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Nelson, Jennifer C. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Ottaway, Heather L. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Paterson, Lisa E. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Revelli, Michael A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Rosene, Crystal A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Terrill, Alison A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Wegrecki, Anthony M. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Wilson, Kent R. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Woollett, Jim S. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2013-09-19

    Lawrence Livermore National Laboratory (LLNL) is a premier research laboratory that is part of the National Nuclear Security Administration (NNSA) within the U.S. Department of Energy (DOE). As a national security laboratory, LLNL is responsible for ensuring that the nation’s nuclear weapons remain safe, secure, and reliable. The Laboratory also meets other pressing national security needs, including countering the proliferation of weapons of mass destruction and strengthening homeland security, and conducting major research in atmospheric, earth, and energy sciences; bioscience and biotechnology; and engineering, basic science, and advanced technology. The Laboratory is managed and operated by Lawrence Livermore National Security, LLC (LLNS), and serves as a scientific resource to the U.S. government and a partner to industry and academia. LLNL operations have the potential to release a variety of constituents into the environment via atmospheric, surface water, and groundwater pathways. Some of the constituents, such as particles from diesel engines, are common at many types of facilities while others, such as radionuclides, are unique to research facilities like LLNL. All releases are highly regulated and carefully monitored. LLNL strives to maintain a safe, secure and efficient operational environment for its employees and neighboring communities. Experts in environment, safety and health (ES&H) support all Laboratory activities. LLNL’s radiological control program ensures that radiological exposures and releases are reduced to as low as reasonably achievable to protect the health and safety of its employees, contractors, the public, and the environment. LLNL is committed to enhancing its environmental stewardship and managing the impacts its operations may have on the environment through a formal Environmental Management System. The Laboratory encourages the public to participate in matters related to the Laboratory’s environmental impact on the

  11. Lawrence Livermore National Laboratory Environmental Report 2013

    Energy Technology Data Exchange (ETDEWEB)

    Jones, H. E. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Bertoldo, N. A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Blake, R. G. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Cerruti, S. J. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Dibley, V. R. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Doman, J. L. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Fish, C. B. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Grayson, A. R. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Heidecker, K. R. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Kumamoto, G. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); MacQueen, D. H. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Montemayor, W. E. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Ottaway, H. L. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Paterson, L. E. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Revelli, M. A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Rosene, C. A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Terrill, A. A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Wegrecki, A. M. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Wilson, K. R. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Woollett, J. S. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Veseliza, R. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2014-10-01

    Lawrence Livermore National Laboratory (LLNL) is a premier research laboratory that is part of the National Nuclear Security Administration (NNSA) within the U.S. Department of Energy (DOE). As a national security laboratory, LLNL is responsible for ensuring that the nation’s nuclear weapons remain safe, secure, and reliable. The Laboratory also meets other pressing national security needs, including countering the proliferation of weapons of mass destruction and strengthening homeland security, and conducting major research in atmospheric, earth, and energy sciences; bioscience and biotechnology; and engineering, basic science, and advanced technology. The Laboratory is managed and operated by Lawrence Livermore National Security, LLC (LLNS), and serves as a scientific resource to the U.S. government and a partner to industry and academia. LLNL operations have the potential to release a variety of constituents into the environment via atmospheric, surface water, and groundwater pathways. Some of the constituents, such as particles from diesel engines, are common at many types of facilities while others, such as radionuclides, are unique to research facilities like LLNL. All releases are highly regulated and carefully monitored. LLNL strives to maintain a safe, secure and efficient operational environment for its employees and neighboring communities. Experts in environment, safety and health (ES&H) support all Laboratory activities. LLNL’s radiological control program ensures that radiological exposures and releases are reduced to as low as reasonably achievable to protect the health and safety of its employees, contractors, the public, and the environment. LLNL is committed to enhancing its environmental stewardship and managing the impacts its operations may have on the environment through a formal Environmental Management System. The Laboratory encourages the public to participate in matters related to the Laboratory’s environmental impact on the

  12. ¿En defensa de la Doctrina Monroe? Los desencuentros en América Latina entre España y Estados Unidos (1880-1890)

    OpenAIRE

    2016-01-01

    This article analyzes the role played by the Monroe Doctrine in the disagreements that took place between Spain and the United States in Latin America during the 1880s. Based on diplomatic sources preserved in archives in the United States and Spain, the article argues that these apparently peripheral incidents reveal that the Monroe Doctrine was a lasting, although flexible, principle of U.S. foreign policy in Latin America. Sspecifically, the evidence suggests that the motor behind the evol...

  13. Mesocarnivore Surveys on Lawrence Livermore National Laboratory Site 300, Alameda and San Joaquin Counties, California

    Energy Technology Data Exchange (ETDEWEB)

    Clark, H O; Smith, D A; Cypher, B L; Kelly, P A; Woollett, J S

    2004-11-16

    Lawrence Livermore National Laboratory (LLNL), operated under cooperative agreement between the University of California and the U. S. Department of Energy, administers and operates an approximately 11 mi{sup 2} (28 km{sup 2}) test site in the remote hills at the northern end of the South Coast Ranges of Central California (Figure 1). Known as Site 300, this expanse of rolling hills and canyons supports a diverse array of grassland communities typical of lowland central California. The facility serves a variety of functions related to testing non-nuclear explosives, lasers, and weapons subsystems. The primary purpose of this project was to determine the presence of any mesocarnivores on Site 300 that use the property for foraging, denning, and other related activities. The surveys occurred from mid-September to mid-October, 2002.

  14. Implementing a Compressed Air System Leak Management Program at an Automotive Plant (Visteon's Monroe Plant)

    Energy Technology Data Exchange (ETDEWEB)

    None

    2001-01-01

    The energy team at Visteon’s Monroe plant, formerly owned by Ford Motor Company, implemented an ongoing compressed air system leak management program. The team developed an approach that combined a traditional “find and fix” effort with an innovative implementation and marketing program. As a result of the leak management program, compressed air system consumption was reduced by more than 50% on a per production unit basis.

  15. The decline of the Monroe Doctrine? Colombia and Brazil, between a traditional north and a renewed south

    OpenAIRE

    2015-01-01

    Since the formulation of the Monroe Doctrine, United States defined Latin America as his ‘backyard’in the XIXth and XXth centuries. However, the global and regional transformations in the XXIst century have weakened the Doctrine, and promoted a new idea of the space, named South America. This paper analyzes the role of Brazil as a regional power, which leads the creation of this new space. Furthermore, it analyzes the role of Colombia, a United States traditional ally, facing the Brazilian le...

  16. Early days in the Lawrence Laboratory. [Through 1940

    Energy Technology Data Exchange (ETDEWEB)

    McMillan, E.M.

    1976-10-01

    Events at the Lawrence Radiation Laboratory at Berkeley to the end of 1940 are recalled. Radiation detection, discovery of new isotopes and elements, and accelerators are among the subjects included. 29 photographs. (RWR)

  17. Chemical analysis of sediments from the St. Lawrence River

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This report provides the results of a preliminary study of sediment contaminant levels in the St. Lawrence River. Sediment was sampled at 1 6 different locations...

  18. Former Fermilab boss to lead Lawrence Berkeley National Laboratory

    Science.gov (United States)

    Gwynne, Peter

    2016-03-01

    Particle physicist Michael Witherell - current vice-chancellor for research at the University of California, Santa Barbara (UCSB) - has been appointed the next director of the Lawrence Berkeley National Laboratory (LBL).

  19. Lawrence Livermore National Laboratory 2007 Annual Report

    Energy Technology Data Exchange (ETDEWEB)

    Chrzanowski, P; Walter, K

    2008-04-25

    Lawrence Livermore National Laboratory's many outstanding accomplishments in 2007 are a tribute to a dedicated staff, which is shaping the Laboratory's future as we go through a period of transition and transformation. The achievements highlighted in this annual report illustrate our focus on the important problems that affect our nation's security and global stability, our application of breakthrough science and technology to tackle those problems, and our commitment to safe, secure, and efficient operations. In May 2007, the Department of Energy (DOE) awarded Lawrence Livermore National Security, LLC (LLNS), a new public-private partnership, the contract to manage and operate the Laboratory starting in October. Since its inception in 1952, the Laboratory had been managed by the University of California (UC) for the DOE's National Nuclear Security Administration (NNSA) and predecessor organizations. UC is one of the parent organizations that make up LLNS, and UC's presence in the new management entity will help us carry forward our strong tradition of multidisciplinary science and technology. 'Team science' applied to big problems was pioneered by the Laboratory's co-founder and namesake, Ernest O. Lawrence, and has been our hallmark ever since. Transition began fully a year before DOE's announcement. More than 1,600 activities had to be carried out to transition the Laboratory from management by a not-for-profit to a private entity. People, property, and procedures as well as contracts, formal agreements, and liabilities had to be transferred to LLNS. The pre-transition and transition teams did a superb job, and I thank them for their hard work. Transformation is an ongoing process at Livermore. We continually reinvent ourselves as we seek breakthroughs that impact emerging national needs. An example is our development in the late 1990s of a portable instrument that could rapidly detect DNA signatures, research that

  20. Lawrence Livermore National Laboratory 2007 Annual Report

    Energy Technology Data Exchange (ETDEWEB)

    Chrzanowski, P; Walter, K

    2008-04-25

    Lawrence Livermore National Laboratory's many outstanding accomplishments in 2007 are a tribute to a dedicated staff, which is shaping the Laboratory's future as we go through a period of transition and transformation. The achievements highlighted in this annual report illustrate our focus on the important problems that affect our nation's security and global stability, our application of breakthrough science and technology to tackle those problems, and our commitment to safe, secure, and efficient operations. In May 2007, the Department of Energy (DOE) awarded Lawrence Livermore National Security, LLC (LLNS), a new public-private partnership, the contract to manage and operate the Laboratory starting in October. Since its inception in 1952, the Laboratory had been managed by the University of California (UC) for the DOE's National Nuclear Security Administration (NNSA) and predecessor organizations. UC is one of the parent organizations that make up LLNS, and UC's presence in the new management entity will help us carry forward our strong tradition of multidisciplinary science and technology. 'Team science' applied to big problems was pioneered by the Laboratory's co-founder and namesake, Ernest O. Lawrence, and has been our hallmark ever since. Transition began fully a year before DOE's announcement. More than 1,600 activities had to be carried out to transition the Laboratory from management by a not-for-profit to a private entity. People, property, and procedures as well as contracts, formal agreements, and liabilities had to be transferred to LLNS. The pre-transition and transition teams did a superb job, and I thank them for their hard work. Transformation is an ongoing process at Livermore. We continually reinvent ourselves as we seek breakthroughs that impact emerging national needs. An example is our development in the late 1990s of a portable instrument that could rapidly detect DNA signatures, research that

  1. Direct utilization of geothermal resources field experiments at Monroe, Utah. Final report, July 14, 1978-July 13, 1981

    Energy Technology Data Exchange (ETDEWEB)

    Blair, C.K.; Owen, L.B. (eds.)

    1982-12-01

    The City of Monroe, Utah undertook a project to demonstrate the economic and technical viability of utilizing a low temperature geothermal resource to provide space and hot water heating to commercial, municipal, and domestic users within the community. During the course of the project, resource development and assessment, including drilling of a production well, was successfully completed. Upon completion of the field development and assessment phase of the program and of a preliminary design of the district heating system, it was determined that the project as proposed was not economically viable. This was due to: (1) a significant increase in estimated capital equipment costs resulting from the general inflation in construction costs, the large area/low population density in Monroe, and a more remote fluid disposal well site than planned, could not balance increased construction costs, (2) a lower temperature resource than predicted, and (3) due to predicted higher pumping and operating costs. After a thorough investigation of alternatives for utilizing the resource, further project activities were cancelled because the project was no longer economical and an alternative application for the resource could not be found within the constraints of the project. The City of Monroe, Utah is still seeking a beneficial use for the 600 gpm, 164/sup 0/F geothermal well. A summary of project activities included.

  2. Lawrence hydroelectric project: resurrection of a low-head dam

    Energy Technology Data Exchange (ETDEWEB)

    1982-04-01

    The Lawrence Hydroelectric Project, located on the Merrimac River in Lawrence, Mass, is one of the first hydro projects completed since the oil crisis and has set an important precedent for the development of other low-head generation systems in New England. The hydroelectric project is built alongside the old 920-ft-long granite dam. The plant uses two bulb-type Kaplan turbine/generator units, each rated at 8.4 MW.

  3. Annual environmental monitoring report of the Lawrence Berkeley Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Schleimer, G.E.; Pauer, R.O. (eds.)

    1990-08-01

    The Lawrence Berkeley Laboratory (LBL) is a multiprogram national laboratory managed by the University of California (UC) for the US Department of Energy (DOE). LBL's major role is to conduct basic and applied science research that is appropriate for an energy research laboratory. The Environmental Monitoring Program of the Lawrence Berkeley Laboratory is described. Data for 1989 are presented, and general trends are discussed. 17 refs., 12 figs., 23 tabs.

  4. On The Attitude Of D.H. Lawrence Towards Feminism

    Institute of Scientific and Technical Information of China (English)

    贾晓哲

    2013-01-01

    D.H. Lawrence, an assumed advocator of Women’s Liberation Movement, has impressively created, out of his evalua-tion of the male and the female, harmonious relationships between man and woman. But influenced by the conventional ando-centric culture, D.H. Lawrence 's consciousness of male superiority is actually all the way working in his writings;leading him to the creation of alienated women characters that can only find their happiness in female obedience and concession.

  5. Pharmaco-miR

    DEFF Research Database (Denmark)

    Rukov, Jakob Lewin; Wilentzik, Roni; Jaffe, Ishai;

    2014-01-01

    curated from the literature, can be searched and downloaded via Pharmaco-miR and informs on trends and generalities published in the field. Overall, we present examples of how Pharmaco-miR provides possible explanations for previously published observations, including how the cisplatin and 5-fluorouracil...... links miRNA expression and drug function by combining data on miRNA targeting and protein-drug interactions. miRNA targeting information derive from both experimental data and computational predictions, and protein-drug interactions are annotated by the Pharmacogenomics Knowledge base (Pharm...... resistance induced by miR-148a may be caused by miR-148a targeting of the gene KIT. The information is available at www.Pharmaco-miR.org....

  6. Obituary: Fred Lawrence Whipple, 1906-2004

    Science.gov (United States)

    Yeomans, Donald Keith

    2004-12-01

    Fred Whipple, one of the founding fathers of planetary science, died on August 30, 2004 just two months shy of his 98th birthday. The breadth of Fred's published research from 1927 through 2000 is quite extraordinary. Although his collected works were published in two massive volumes in 1972, shortly before his retirement, Fred's research contributions continued for another three decades - and another volume is planned. Fred Lawrence Whipple was born on November 5, 1906 on a farm in Red Oak Iowa. His parents were Harry Lawrence and Celestia (MacFarl) Whipple. At the age of fifteen, the Whipple family moved to California where Fred studied mathematics at Occidental College and the University of California at Los Angeles. As a graduate student at the University of California at Berkeley in 1930, he was one of the first to compute an orbit for the newly discovered planet Pluto. Upon receiving his PhD in 1931, he joined the staff of the Harvard College Observatory. He was Chairman of the Harvard Department of Astronomy (1949 - 1956), Director or the Smithsonian Astrophysical Observatory (1955 - 1973), Phillips Professor of Astronomy (1968 - 1977) and Emeritus Phillips Professor of astronomy (1977 - 2004). In 1928 he married Dorothy Woods and their son, Earle Raymond, survives him. The marriage ended in divorce in 1935. Eleven years later, he married Babette F. Samelson and she too survives him, as do their two daughters Laura and (Dorothy) Sandra. Shortly after arriving at Harvard in the early 1930's, Fred developed a photographic tracking network to determine meteor trajectories from simultaneous observations from two or more stations. The photographic trails, chopped by a rotating shutter, allowed their orbits in space to be determined accurately. With the strong involvement of Richard McCrosky and others, he concluded in the early 1960's that most of these meteors were on comet-like orbits and less than 1% of the naked eye, sporadic meteors could be traced to an

  7. Industrial hygiene walk-through survey report of General Motors Corporation, Fisher Guide Division, Monroe, Louisiana

    Energy Technology Data Exchange (ETDEWEB)

    McCammon, C.S.; Krishnan, E.R.; Goodman, R.J.

    1987-08-17

    A walk-through industrial-hygiene survey was conducted at the General Motors Corporation Fisher Guide manufacturing facility located in Monroe, Louisiana. The investigation centered on possible exposure to acrylates or methacrylates during the manufacture of adhesive-sealed, flame-sealed, or composite headlamps. Methacrylate-based adhesives and acrylated coatings have been used at the facility since 1981. Both the adhesives and coatings were cured by ultraviolet radiation. Ninety-four employees had potential contact with acrylates or methacrylates during the application of adhesives and coatings. Adhesives were used for sealing the lens/reflector area and the bulb location of the terminal area. Base coats were applied to the composite headlamps to provide an adhering surface for the aluminized coating. Top coats were applied as a protective coating for the aluminum coating. Two cases of chemical dermatitis have been reported at the facility since 1981 due to skin contact with acrylates/methacrylates. When an automated system was used to apply adhesive materials during headlamp assembly, there was a lower potential for employee exposure to the hazardous chemicals. The authors recommend that the company move toward complete automation of this part of the production process. Levels of butyl-acetate should be checked as there was a strong smell of this chemical in part of the production line.

  8. A 2-D simulation of hydrocephalus in the Foramens of Monro of the human ventricular system

    Energy Technology Data Exchange (ETDEWEB)

    Ammourah, S.; Aroussi, A. [Univ. of Nottingham, School of Mechanical, Materials, Manufacturing and Management, University Park, Nottingham (United Kingdom)]. E-mail: eaxsaka@nottingham.ac.uk; Vloeberghs, M. [Queen' s Medical Centre, Dept. of Child Health, Nottingham (United Kingdom)

    2004-07-01

    This study investigates the Cerebrospinal fluid (CSF) flow behavior in a two-dimensional plane (2-D) of the human ventricular system when the hydrocephalus in the Foramens of Monro (F.O.M) occurs. In order to understand the CSF behaviour when the hydrocephalus occurs, it is essential to comprehend its normal flow dynamics i.e. the healthy case with no hydrocephalus. This had been done numerically by reconstructing the ventricular system geometry from the MRI scans and then made a 15{sup o} degree cut in the surgical line to obtain the outlines of the 2-D plane. The healthy cases were solved for the CSF actual flow rate, which is between 100-500 ml/day in 100 incremental steps. The unhealthy cases were studied for the average flow rate, which is 300 ml/day but for blockage ratios of the Foramen diameter of 20%, 50% and 75%. These obstructions are designed to simulate the hydrocephalus. The results show that as the flow rate increases the pressure and the velocity values increases, but no changes in the flow pattern occurs. The maximum pressure on the normal cases occurred in the lateral ventricles and the maximum velocity occurred in the aqueduct neck. The results of the hydrocephalus cases show that as the obstruction degree increases the pressure in the lateral ventricle increases accompanied with a velocity increase in the obstructed Foramen, which may cause unwanted stress on the neighboring tissues. (author)

  9. Idiopathic bilateral occlusion of the foramen of Monro: An unusual entity with varied clinical presentations.

    Science.gov (United States)

    Mizrahi, Cezar J; Cohen, José E; Gomori, J M; Shoshan, Yigal; Spektor, Sergey; Moscovici, Samuel

    2016-12-01

    We review our experience with four patients who presented to our Medical Center from 2005-2015 with adult idiopathic occlusion of the foramen of Monro (FM). All patients underwent CT scanning and MRI. Standard MRI was performed in each patient to rule out a secondary cause of obstruction (T1-weighted without- and with gadolinium, T2-weighted, fluid-attenuated inversion recovery [FLAIR] and diffusion-weighted imaging [DWI] protocols). When occlusion of the FM appeared to be idiopathic, further high-resolution MRI with multiplanar reconstructions for evaluation of stenosis or an occluding membrane at the level of the FM was performed (T1-weighted without- and with gadolinium, T2-weighted 3D turbo spin-echo). Occlusion of the FM was due to unilateral stenosis and septum pellucidum deviation in two patients, to an occluding membrane in one, and to bilateral stenosis in one patient. Urgent surgical intervention is mandatory when there are signs of increased intracranial pressure while asymptomatic patients may be managed conservatively. In this patient series, truly bilateral stenotic obstruction of the FM was best managed with ventriculoperitoneal shunt and patients with membranous obstruction or unilateral stenosis with septum deviation were treated endoscopically. Copyright © 2016 Elsevier Ltd. All rights reserved.

  10. Lawrence Livermore National Laboratory Annual Report 2006

    Energy Technology Data Exchange (ETDEWEB)

    Chrzanowski, P; Walter, K

    2007-05-24

    For the Laboratory and staff, 2006 was a year of outstanding achievements. As our many accomplishments in this annual report illustrate, the Laboratory's focus on important problems that affect our nation's security and our researchers breakthroughs in science and technology have led to major successes. As a national laboratory that is part of the Department of Energy's National Nuclear Security Administration (DOE/NNSA), Livermore is a key contributor to the Stockpile Stewardship Program for maintaining the safety, security, and reliability of the nation's nuclear weapons stockpile. The program has been highly successful, and our annual report features some of the Laboratory's significant stockpile stewardship accomplishments in 2006. A notable example is a long-term study with Los Alamos National Laboratory, which found that weapon pit performance will not sharply degrade from the aging effects on plutonium. The conclusion was based on a wide range of nonnuclear experiments, detailed simulations, theoretical advances, and thorough analyses of the results of past nuclear tests. The study was a superb scientific effort. The continuing success of stockpile stewardship enabled NNSA in 2006 to lay out Complex 2030, a vision for a transformed nuclear weapons complex that is more responsive, cost efficient, and highly secure. One of the ways our Laboratory will help lead this transformation is through the design and development of reliable replacement warheads (RRWs). Compared to current designs, these warheads would have enhanced performance margins and security features and would be less costly to manufacture and maintain in a smaller, modernized production complex. In early 2007, NNSA selected Lawrence Livermore and Sandia National Laboratories-California to develop ''RRW-1'' for the U.S. Navy. Design efforts for the RRW, the plutonium aging work, and many other stockpile stewardship accomplishments rely on computer

  11. Ernest Orlando Lawrence (1901-1958), Cyclotron and Medicine

    Energy Technology Data Exchange (ETDEWEB)

    Chu, William T.

    2005-09-01

    On August 8, 2001, Lawrence Berkeley National Laboratory celebrated the centennial of the birth of its founder (and namesake), Ernest Orlando Lawrence. For the occasion, many speeches were given and old speeches were remembered. We recall the words of the late Luis Alvarez, a Nobel Laureate and one of the Lawrence's closest colleagues: ''Lawrence will always be remembered as the inventor of the cyclotron, but more importantly, he should be remembered as the inventor of the modern way of doing science''. J. L. Heilbron and R. W. Seidel, in the introduction of their book, ''Lawrence and His Laboratory'' stated, ''The motives and mechanisms that shaped the growth of the Laboratory helped to force deep changes in the scientific estate and in the wider society. In the entrepreneurship of its founder, Ernest Orlando Lawrence, these motives, mechanisms, and changes came together in a tight focus. He mobilized great and small philanthropists, state and local governments, corporations, and plutocrats, volunteers and virtuosos. The work they supported, from astrophysics and atomic bombs, from radiochemistry to nuclear medicine, shaped the way we observe, control, and manipulate our environment.'' Indeed, all over the civilized world, the ways we do science changed forever after Lawrence built his famed Radiation Laboratory. In this editorial, we epitomize his legacy of changing the way we do medicine, thereby affecting the health and well being of all humanity. This year marks the 75th anniversary of the invention of the cyclotron by Ernest Orlando Lawrence at the University of California at Berkeley. Lawrence conceived the idea of the cyclotron early in 1929 after reading an article by Rolf Wideroe on high-energy accelerators. In the spring of 1930 one of his students, Nels Edlefsen, constructed two crude models of a cyclotron. Later in the fall of the same year, another student, M. Stanley Livingston

  12. Lawrence Livermore National Laboratory Annual Report 2006

    Energy Technology Data Exchange (ETDEWEB)

    Chrzanowski, P; Walter, K

    2007-05-24

    For the Laboratory and staff, 2006 was a year of outstanding achievements. As our many accomplishments in this annual report illustrate, the Laboratory's focus on important problems that affect our nation's security and our researchers breakthroughs in science and technology have led to major successes. As a national laboratory that is part of the Department of Energy's National Nuclear Security Administration (DOE/NNSA), Livermore is a key contributor to the Stockpile Stewardship Program for maintaining the safety, security, and reliability of the nation's nuclear weapons stockpile. The program has been highly successful, and our annual report features some of the Laboratory's significant stockpile stewardship accomplishments in 2006. A notable example is a long-term study with Los Alamos National Laboratory, which found that weapon pit performance will not sharply degrade from the aging effects on plutonium. The conclusion was based on a wide range of nonnuclear experiments, detailed simulations, theoretical advances, and thorough analyses of the results of past nuclear tests. The study was a superb scientific effort. The continuing success of stockpile stewardship enabled NNSA in 2006 to lay out Complex 2030, a vision for a transformed nuclear weapons complex that is more responsive, cost efficient, and highly secure. One of the ways our Laboratory will help lead this transformation is through the design and development of reliable replacement warheads (RRWs). Compared to current designs, these warheads would have enhanced performance margins and security features and would be less costly to manufacture and maintain in a smaller, modernized production complex. In early 2007, NNSA selected Lawrence Livermore and Sandia National Laboratories-California to develop ''RRW-1'' for the U.S. Navy. Design efforts for the RRW, the plutonium aging work, and many other stockpile stewardship accomplishments rely on computer

  13. Storms, hanged pirates, anemia, exsanguination: the contributions of Monro, Kellie and Abercrombie in understanding intracranial blood circulation

    Directory of Open Access Journals (Sweden)

    Pasquale De Bonis

    2014-12-01

    Full Text Available The so-called Monro-Kellie doctrine states that with an intact skull, the sum of the volume of the brain plus the cerebrospinal fluid volume plus the intracranial blood volume is constant. Therefore an increase in one should cause a reduction in one or both of the remaining two. The researcher who expressed the doctrine in such a way was indeed Harvey Cushing in 1925, during a lecture in Edinburgh. The original Monro-Kellie doctrine is the result of observations on autopsies and several animal experiments. What the original doctrine states is a dynamic explanation of the intracranial system, aimed at explaining how a pulsatile and continuous perfusion may occur in a closed-non-expandable and fully filled system. During each cardiac cycle the quantity of blood within the head must be the same: during the systole, the brain arteries dilate, and, in the mean time, a quantity of blood, equal to that which is dilating them, is passing out of the head through the veins. During the succeeding diastole, the quantity which dilated the brain arteries passes into the corresponding veins and, at the same time, as much passes from the sinuses out of the head, as enters into the head from the arteries situated between the heart and the head. Monro implicitly states that the blood coming out from the cerebral veins into the sinuses must be pulsatile and (almost synchronous with the arteries. That is deeply different from the concept of a constant content of a rigid case, as expressed by Cushing.

  14. Colombia y la Doctrina Monroe: el caso del Ferrocarril de Panamá y las intervenciones estadounidenses en el Istmo

    OpenAIRE

    2014-01-01

    La construcción y puesta en marcha del Ferrocarril de Panamá se constituyó como uno de los procesos de inversión extranjera en América Latina durante el siglo XIX. Este ferrocarril, construido como una forma de superar las dificultades de navegación entre el mar Caribe y el Océano Pacífico, permite evidenciar el despliegue de la Doctrina Monroe sobre América Latina y el Caribe, la interrelación entre los intereses de las élites locales y los intereses internacionales, y la forma en que se est...

  15. El dilema de Colombia en las Américas entre el monroísmo y el sudamericanismo

    OpenAIRE

    2013-01-01

    La alineación con Estados Unidos es uno de los rasgos más constantes de la política exterior colombiana. Sin embargo, el paso del orden bipolar a uno multipolar, la emergencia de Brasil como potencia regional, y la llegada de Juan Manuel Santos a la presidencia de Colombia, pueden estar incitando un cambio respecto a este alineamiento incondicional. Uno de los elementos más significativos en este cambio es el debilitamiento de la Doctrina Monroe como patrón de relacionamiento entre Colombia y...

  16. ¿En defensa de la Doctrina Monroe? Los desencuentros en América Latina entre España y Estados Unidos (1880-1890

    Directory of Open Access Journals (Sweden)

    Andrés Sánchez Padilla

    2016-10-01

    Full Text Available This article analyzes the role played by the Monroe Doctrine in the disagreements that took place between Spain and the United States in Latin America during the 1880s. Based on diplomatic sources preserved in archives in the United States and Spain, the article argues that these apparently peripheral incidents reveal that the Monroe Doctrine was a lasting, although flexible, principle of U.S. foreign policy in Latin America. Sspecifically, the evidence suggests that the motor behind the evolution of the doctrine was U.S. domestic policy.

  17. O caso Marylin Monroe: evidências da forclusão do sujeito e de seu ato Marylin Monroe's case: evidences of the subject's Verwerfung and of its act

    Directory of Open Access Journals (Sweden)

    Jéferson Machado Pinto

    2012-06-01

    Full Text Available A objetivação da transferência sustenta uma posição epistemológica característica do discurso da ciência e implica a forclusão do real do sujeito e de sua verdade, excluindo a possibilidade do ato analítico. Para mostrá-lo, o trabalho utiliza dados biográficos e relatos das últimas sessões da análise de Marylin Monroe. Destaca que o masoquismo, vertente real do enlaçamento amoroso da atriz com o Outro, foi elidido em favor da interpretação simbólica do inconsciente e da transferência tomada como simples repetição das relações de objeto, com trágicas consequências para o sujeito.The transfer objectification is based on an epistemological position characteristic of the science discourse and implies the Verwerfung of the subject's real and of its truth, excluding the possibility of the analytical act. To discuss it, this paper uses biographical data and reports of Marylin Monroe's last analytical sessions emphasizing that masochism, the real part of the actress' love engagements with the Other, was ignored in favor of the symbolic interpretation of the unconscious and of the transfer taken as a mere repetition of the object relationships, with tragic consequences on the subject.

  18. Environmental monitoring at the Lawrence Livermore Laboratory. 1979 annual report

    Energy Technology Data Exchange (ETDEWEB)

    Silver, W.J.; Lindeken, C.L.; White, J.H.; Buddemeir, R.W.

    1980-04-25

    Information on monitoring activities is reported in two sections for EDB/ERA/INIS. The first section covers all information reported except Appendix D, which gives details of sampling and analytical procedures for environmental monitoring used at Lawrence Livermore Laboratory. A separate abstract was prepared for Appendix D. (JGB)

  19. Crystal clear the autobiographies of Sir Lawrence and Lady Bragg

    CERN Document Server

    Thomson, Patience

    2015-01-01

    The main body of this book contains the hitherto unpublished autobiographies of both William Lawrence Bragg, an innovative scientist who won the Nobel Prize for Physics in 1915, and his wife, Alice, a Mayor of Cambridge and National Chairman of Marriage Guidance. Their autobiographies give unusual insights into the lives and times of two distinguished people and the real personalities behind their public appearance.

  20. Lawrence Berkeley Laboratory Institutional Plan FY 1995--2000

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1994-12-01

    This report presents the details of the mission and strategic plan for Lawrence Berkeley Laboratory during the fiscal years of 1995--2000. It presents summaries of current programs and potential changes; critical success factors such as human resources; management practices; budgetary allowances; and technical and administrative initiatives.

  1. Waste management study: Process development at Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    1984-12-01

    This report presents the results of an evaluation of the present Toxic Waste Control Operations at the Lawrence Livermore National Laboratory, evaluates the technologies most applicable to the treatment of toxic and hazardous wastes and presents conceptual designs of processes for the installation of a new decontamination and waste treatment facility (DWTF) for future treatment of these wastes.

  2. Jacob Lawrence's "The Migration Series": Art as Narrative History

    Science.gov (United States)

    Laney, James D.

    2007-01-01

    Because art is a reflection of cultural heritage, a natural affinity exists between art and social studies. In Jacob Lawrence's "The Migration Series," art serves as narrative history, with visual images telling the story of the Great Migration, a movement of African American people from the South to the North around World War I. Social studies…

  3. An Interview with Lawrence Greenberg about Attention Deficit and Hyperactivity.

    Science.gov (United States)

    Shaughnessy, Michael F.; Martin, Jeremy; Rivera, Hipolito

    1999-01-01

    Presents an interview with pediatrician and adult and child psychiatrist Dr. Lawrence Greenberg, researcher, clinician, and expert on attention deficit, hyperactivity, impulsivity, and related concerns. Discusses the TOVA (Test of Variables of Attention), a continuous performance test for clinical use developed by Dr. Greenberg; diagnostic…

  4. D.H.Lawrence's Views On European Industrial Civilization

    Institute of Scientific and Technical Information of China (English)

    冯建明

    2001-01-01

    In Women in Love (1921)the reader can easily perceive the author's abhorrence to European industrial civilization T Lawrence, the inescapable fate of this sort of social deveolpment is destruction And he takes it for granted that"Rananim"--the Lawrencian utopia, is a social outlet for human salvation.

  5. Colloid cysts posterior and anterior to the foramen of Monro: Anatomical features and implications for endoscopic excision

    Directory of Open Access Journals (Sweden)

    Waleed A Azab

    2014-01-01

    Full Text Available Background: Colloid cysts are usually located at the rostral part of the third ventricle in proximity to the foramina of Monro. Some third ventricular colloid cysts, however, attain large sizes, reach a very high distance above the roof of the third ventricle, and pose some challenges during endoscopic excision. These features led to the speculation that for such a pattern of growth to take place, the points of origin of these cysts should be at areas away from the foramina of Monro at which some anatomical "windows" exist that are devoid of compact, closely apposed forniceal structures. Methods: A review of the literature on anatomical variations of the structures in the vicinity of the roof of the third ventricle and on reported cases with similar features was conducted. Results: Colloid cysts may grow vertically up past the roof of the third ventricle through anatomical windows devoid of the mechanical restraint of the forniceal structures. Conclusion: Some anatomical variations of the forniceal structures may allow unusually large sizes and superior vector of growth of a retro- or post-foraminal colloid cyst. Careful preoperative planning and knowledge of the pertinent pathoanatomy of these cysts before endoscopic excision is very important to avoid complications.

  6. From "Lawrence Kohlberg's Approach to Moral Education" by F. Clark Power, Ann Higgins, and Lawrence Kohlberg, with Judy Codding (1989)

    Science.gov (United States)

    Schools: Studies in Education, 2011

    2011-01-01

    This article is an excerpt from "Lawrence Kohlberg's Approach to Moral Education." It refers several times to Kohlberg's "six stages of moral development." Stages 3 and 4 belong to the second level of moral development, which Kohlberg calls "conventional." At stage 3, one becomes aware of conventions as one sees what is right in terms of living up…

  7. Estimation of Contextual Effects through Nonlinear Multilevel Latent Variable Modeling with a Metropolis-Hastings Robbins-Monro Algorithm. CRESST Report 833

    Science.gov (United States)

    Yang, Ji Seung; Cai, Li

    2013-01-01

    The main purpose of this study is to improve estimation efficiency in obtaining full-information maximum likelihood (FIML) estimates of contextual effects in the framework of a nonlinear multilevel latent variable model by adopting the Metropolis-Hastings Robbins-Monro algorithm (MH-RM; Cai, 2008, 2010a, 2010b). Results indicate that the MH-RM…

  8. MiNube

    OpenAIRE

    Villanueva Hernández, Yarilo David

    2015-01-01

    [ES]MiNube es una solución de almacenamiento de datos online enfocada en la privacidad, seguridad y control del usuario final. A diferencia de otras plataformas de almacenamiento en la nube en el que se depende de agentes externos, MiNube cede el control de los datos al usuario, permitiéndole configurar su propio entorno y definiendo políticas de encriptación en las que el propio usuario es el único agente capaz de acceder a sus datos. MiNube se compone de tres partes: Un servidor web para s...

  9. DE TIAM MI ESTAS ESPERANTISTO

    Institute of Scientific and Technical Information of China (English)

    Verkis V.Setala

    1996-01-01

    En 1903, iufoje mi vojais de Bukareto alKonstantinpolo, kaj mi prenis sidlokon en larestoracia vagono. Apenaǚ mi sidiis, mividis ke iu eksterlandano penas komprenigi alkelnero kion li deziras. La kelnero uzas sian tu-tan lingvoscion: li parolas rumane, serbe,turke, bulgare kaj ruse, sed vane! La fremd-landano ne komprenas e unu vorton. Mi

  10. Guide to user facilities at the Lawrence Berkeley Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    1984-04-01

    Lawrence Berkeley Laboratories' user facilities are described. Specific facilities include: the National Center for Electron Microscopy; the Bevalac; the SuperHILAC; the Neutral Beam Engineering Test Facility; the National Tritium Labeling Facility; the 88 inch Cyclotron; the Heavy Charged-Particle Treatment Facility; the 2.5 MeV Van de Graaff; the Sky Simulator; the Center for Computational Seismology; and the Low Background Counting Facility. (GHT)

  11. Astronomy applications of adaptive optics at Lawrence Livermore National Laboratory

    Science.gov (United States)

    Bauman, Brian J.; Gavel, Donald T.

    2003-06-01

    Astronomical applications of adaptive optics at Lawrence Livermore National Laboratory (LLNL) has a history that extends from 1984. The program started with the Lick Observatory Adaptive Optics system and has progressed through the years to lever-larger telescopes: Keck, and now the proposed CELT (California Extremely Large Telescope) 30m telescope. LLNL AO continues to be at the forefront of AO development and science.

  12. Astronomy Applications of Adaptive Optics at Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Bauman, B J; Gavel, D T

    2003-04-23

    Astronomical applications of adaptive optics at Lawrence Livermore National Laboratory (LLNL) has a history that extends from 1984. The program started with the Lick Observatory Adaptive Optics system and has progressed through the years to lever-larger telescopes: Keck, and now the proposed CELT (California Extremely Large Telescope) 30m telescope. LLNL AO continues to be at the forefront of AO development and science.

  13. Catalog of research projects at Lawrence Berkeley Laboratory, 1985

    Energy Technology Data Exchange (ETDEWEB)

    1985-01-01

    This Catalog has been created to aid in the transfer of technology from the Lawrence Berkeley Laboratory to potential users in industry, government, universities, and the public. The projects are listed for the following LBL groups: Accelerator and Fusion Research Division, Applied Science Division, Biology and Medicine Division, Center for Advanced Materials, Chemical Biodynamics Division, Computing Division, Earth Sciences Division, Engineering and Technical Services Division, Materials and Molecular Research Division, Nuclear Science Division, and Physics Division.

  14. 'Criticism at the frontier: Lawrence Alloway at the Movies'

    OpenAIRE

    Bradnock, Lucy

    2015-01-01

    This essay examines the role of film viewing and film criticism in the early intellectual formation of the British art critic and curator Lawrence Alloway. Drawing on archival material from the Alloway Papers at the Getty Research Institue, including plans for an early unpublished book on the subject of film, it argues that Alloway's interest in film and film criticism anticipated many of his later views on art, art criticism, and the politics of the art world.

  15. Colombia y la Doctrina Monroe: el caso del Ferrocarril de Panamá y las intervenciones estadounidenses en el Istmo

    Directory of Open Access Journals (Sweden)

    Juan Santiago Correa R

    2014-01-01

    Full Text Available La construcción y puesta en marcha del Ferrocarril de Panamá se constituyó como uno de los procesos de inversión extranjera en América Latina durante el siglo XIX. Este ferrocarril, construido como una forma de superar las dificultades de navegación entre el mar Caribe y el Océano Pacífico, permite evidenciar el despliegue de la Doctrina Monroe sobre América Latina y el Caribe, la interrelación entre los intereses de las élites locales y los intereses internacionales, y la forma en que se estaba definiendo el concepto mismo de nación durante el siglo XIX.

  16. Mi Pueblo Food Center

    Directory of Open Access Journals (Sweden)

    Alexis A. Babb

    2016-04-01

    Full Text Available This case describes a current growth opportunity for Mi Pueblo Food Center, a Hispanic grocery chain with locations throughout the Bay Area, California. The CEO of Mi Pueblo is contemplating opening a new store location in East Palo Alto, CA, which has been without a local, full-service grocery store for over 20 years. Case objectives are for students to develop an understanding of how the grocery industry operates, the risks and opportunities associated with opening a new grocery store location, and the impact on social, environmental, and economic sustainability. The SWOT (Strengths, Weaknesses, Opportunities, Threats framework is used to analyze whether or not it is feasible for Mi Pueblo to open a new location in East Palo Alto. This case may be used with students in graduate and advanced undergraduate courses.

  17. MiDAS

    DEFF Research Database (Denmark)

    McIlroy, Simon Jon; Saunders, Aaron Marc; Albertsen, Mads

    2015-01-01

    The Microbial Database for Activated Sludge (MiDAS) field guide is a freely available online resource linking the identity of abundant and process critical microorganisms in activated sludge wastewater treatment systems to available data related to their functional importance. Phenotypic properties...... communities. The taxonomy can be used to classify unknown sequences, and the online MiDAS field guide links the identity to the available information about their morphology, diversity, physiology and distribution. The use of a common taxonomy across the field will provide a solid foundation for the study...... of microbial ecology of the activated sludge process and related treatment processes. The online MiDAS field guide is a collaborative workspace intended to facilitate a better understanding of the ecology of activated sludge and related treatment processes—knowledge that will be an invaluable resource...

  18. USING DOE-2.1 AT LAWRENCE BERKELEY LABORATORY

    Energy Technology Data Exchange (ETDEWEB)

    Building Energy Analysis Group.; Authors, Various

    1980-09-01

    The purpose of this manual is to assist the DOE-2 user to run DOE-2 and its utility programs at Lawrence Berkeley Laboratory (LBL). It is organized to reflect the facts that every DOE-2 job run at LBL requires certain steps, and that there are options related to DOE-2 job runs available to any DOE-2 user. The standard steps for running a DOE-2 job are as follows: 1. Prepare a job deck 2. Process a job deck 3. Obtain standard output reports.

  19. Technical Safety Appraisal of the Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    1990-12-01

    This report documents the results of the Technical Safety Appraisal (TSA) of the Lawrence Livermore National Laboratory (LLNL) (including the Site 300 area), Livermore, California, conducted from February 26 to April 5, 1990. The purpose of the assessment was to provide the Secretary of Energy with the status of Environment, Safety and Health (ES H) Programs at LLNL. LLNL is operated by the University of California for the Department of Energy (DOE), and is a multi-program, mission-oriented institution engaged in fundamental and applied research programs that require a multidisciplinary approach. 1 fig.

  20. Lawrence Berkeley National Laboratory 1995 site environmental report

    Energy Technology Data Exchange (ETDEWEB)

    Balgobin, D.; Javandel, I.; Lackner, G.; Smith, C.; Thorson, P.; Tran, H.

    1996-07-01

    The 1995 Site Environmental Report summarizes environmental activities at the Ernest Orlando Lawrence Berkeley National Laboratory (LBNL) for the 1995 calendar year. The report strives to present environmental data in a manner that characterizes the performance and compliance status of the environmental management programs. The report also discusses significant highlights and plans of these programs. Topics discussed include: environmental monitoring, environmental compliance programs, air quality, water quality, ground water protection, sanitary sewer monitoring, soil and sediment quality, vegetation and foodstuffs monitoring, and special studies which include preoperational monitoring of building 85 and 1995 sampling results, radiological dose assessment, and quality assessment.

  1. Optical Design Capabilities at Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Lawson, J K

    2002-12-30

    Optical design capabilities continue to play the same strong role at Lawrence Livermore National Laboratory (LLNL) that they have played in the past. From defense applications to the solid-state laser programs to the Atomic Vapor Laser Isotope Separation (AVLIS), members of the optical design group played critical roles in producing effective system designs and are actively continuing this tradition. This talk will explain the role optical design plays at LLNL, outline current capabilities and summarize a few activities in which the optical design team has been recently participating.

  2. Status of the belugas of the St Lawrence estuary, Canada

    OpenAIRE

    Michael CS Kingsley

    2002-01-01

    A population of belugas (Delphinapterus leucas) inhabiting the estuary of the St Lawrence river in Quebec, Canada, was depleted by unregulated hunting, not closed until 1979. Surveys in 1977 showed only a few hundred in the population. Surveys since then have produced increasing estimates of population indices. An estimate of the population, fully corrected for diving animals, was 1,238 (SE 119) in September 1997. The population was estimated to have increased from 1988 through 1997 by 31.4 b...

  3. True hermaphroditism in a St. Lawrence beluga whale (Delphinapterus leucas).

    Science.gov (United States)

    De Guise, S; Lagacé, A; Béland, P

    1994-04-01

    A hermaphrodite beluga whale (Delphinapterus leucas) was found in the St. Lawrence Estuary, Québec, Canada. This animal had two testicles, two separate ovaries, and the complete ducts of each sex; cervix, vagina and vulva were absent. Mature spermatozoa were found in the lumen of seminiferous tubules in the testicles, and numerous involuted corpora lutea were recognized in the ovaries. This represents the first case of true hermaphroditism in a cetacean, and is the fourth hermaphrodite mammal with two testicles and two separate ovaries.

  4. 75 FR 34711 - Suspension of Nationwide Permit 21

    Science.gov (United States)

    2010-06-18

    ..., Laurel, Lawrence, Lee, Leslie, Letcher, Lewis, Lincoln, McCreary, Madison, Magoffin, Martin, Menifee, Metcalfe, Monroe, Montgomery, Morgan, Nicholas, Owsley, Perry, Pike, Powell, Pulaski, Robertson, Rockcastle..., Lawrence, Mahoning, Meigs, Monroe, Morgan, Muskingum, Noble, Perry, Pike, Ross, Scioto,...

  5. 多视角看爱丽丝·门罗小说的文体特征--以门罗短篇小说《逃离》为例%Stylistic features of Monroe Alice's novels from multiple perspectives--Taking Monroe's short story "escape" as an example

    Institute of Scientific and Technical Information of China (English)

    尉艳英

    2016-01-01

    The novel has its own special style and way of expression, as wel as their own special cultural environment. Different writers have their own creation and writing style, the Alice Monroe's short story "escape", for example, from the point of view of Monroe's language features, narrative technique to explore the stylistic features of the novel Monroe to prove the Monroe novels of psychological realism color, Gothic style, and the feminist and aesthetic reflected.%小说有自己特殊的文体表达规范和方式,还有各自特殊的文化环境。不同的小说作者,又有各自创作和写作风格,本文以爱丽丝·门罗的短篇小说《逃离》为例,从门罗的语言特征、叙事手法等角度,对门罗小说的文体特征进行探究,来印证门罗小说的心理现实主义色彩,哥特式风格,以及其女性主义和美学体现。

  6. Treasury Offset Program (TOP) MI

    Data.gov (United States)

    Social Security Administration — The TOP MI helps OPSOS coordinate TOP case processing in the regions. The MI also helped communicate our progress and findings to BFQM and ORDP, as well as the ACOSS.

  7. Electronic Services Monthly MI Report

    Data.gov (United States)

    Social Security Administration — This electronic services monthly MI report contains monthly MI data for most public facing online online applications such as iClaim, electronic access, Mobile wage...

  8. Tumors in St. Lawrence beluga whales (Delphinapterus leucas)

    Science.gov (United States)

    De Guise, S; Lagacé, A; Béland, P

    1994-07-01

    A population of 450-500 belugas (Delphinapterus leucas) resides in the polluted estuary of the St. Lawrence River. Stranded carcasses of this endangered population were recovered and necropsied. High concentrations of organochlorines, heavy metals, and benzo-a-pyrene exposure were demonstrated in tissues of these whales. Between 1988 and 1990, 21 tumors were found in 12 out of 24 carcasses. Among these tumors, six were malignant and 15 were benign. The animals were between 1.5 and > 29 years of age, and the ages of animals with and without tumors did not differ when two juvenile animals (1.5 and 3.5 years of age) were excluded. Seven other neoplasms had been reported previously in six out of 21 well-preserved carcasses examined in the same laboratory between 1982 and 1987. Overall, 28 of the 75 confirmed tumors reported so far in cetaceans (37%) were from this small population of beluga whales in the St. Lawrence Estuary. Such a high prevalence of tumors would suggest an influence of contaminants through a direct carcinogenic effect and/or a decreased resistance to the development of tumors in this population.

  9. Painter Liu Mi

    Institute of Scientific and Technical Information of China (English)

    1996-01-01

    LIU Mi graduated from the Department of Oil Painting at the Sichuan Academy of Fine Arts. In following years, she worked in stage and wardrobe design for the military song and dance ensemble. She later worked for the Chongqing Foreign Trade Company where her packaging design achievements won

  10. MI 4010 Thermoelectric Modules.

    Science.gov (United States)

    The report covers the design justification, physical specification and characterization of the MI 4010 module . The purpose of the contract was to...demonstrate the capability to fabricate pieceparts, process into assemblies, and test thermoelectric modules equivalent to the module used in the Hand...Held Thermal Viewer. The completed modules were also subjected to limited demonstration tests of reliability and useful life.

  11. MI 6040 Thermoelectric Modules.

    Science.gov (United States)

    The report covers the design justification, physical specification and characterization of the MI 6040 module . The purpose of the thermoelectric... modules is the cooling of infrared detector arrays to temperature of 170K or colder. The completed modules were also subjected to limited demonstration tests of reliability and useful life.

  12. 近二十年来孟禄研究述评%A Review of Monroe Research for the Past Two Decades

    Institute of Scientific and Technical Information of China (English)

    徐浩真

    2014-01-01

    Monroe is an important founder of the historiography of American education, and has greatly promoted the develop-ment and prosperity of the subject. From the 1910s to the 1940s, Monroe came to China for more than 10 times, conducting activi-ties of education survey, discussion and academic lectures. In particular, his visit in 1921 greatly promoted the promulgation of Renxu School System, the reform of secondary education and vocational education, and the development of science education. The paper summarizes and comments on Monroe research for the past two decades to draw scholars' attention to the research and further related studies.%孟禄是20世纪美国教育史学的重要奠基人。他十余次来华进行教育调查、座谈讨论、学术讲演,特别是1921年访华对我国壬戌学制的颁布,中学教育、职业教育的改革,科学教育的发展等起着巨大的推动作用。对近二十年来孟禄研究作一总结与述评,以期引起学者对孟禄研究的重视并推动相关研究的开展。

  13. MiRTargetLink--miRNAs, Genes and Interaction Networks.

    Science.gov (United States)

    Hamberg, Maarten; Backes, Christina; Fehlmann, Tobias; Hart, Martin; Meder, Benjamin; Meese, Eckart; Keller, Andreas

    2016-04-14

    Information on miRNA targeting genes is growing rapidly. For high-throughput experiments, but also for targeted analyses of few genes or miRNAs, easy analysis with concise representation of results facilitates the work of life scientists. We developed miRTargetLink, a tool for automating respective analysis procedures that are frequently applied. Input of the web-based solution is either a single gene or single miRNA, but also sets of genes or miRNAs, can be entered. Validated and predicted targets are extracted from databases and an interaction network is presented. Users can select whether predicted targets, experimentally validated targets with strong or weak evidence, or combinations of those are considered. Central genes or miRNAs are highlighted and users can navigate through the network interactively. To discover the most relevant biochemical processes influenced by the target network, gene set analysis and miRNA set analysis are integrated. As a showcase for miRTargetLink, we analyze targets of five cardiac miRNAs. miRTargetLink is freely available without restrictions at www.ccb.uni-saarland.de/mirtargetlink.

  14. Mi-spillet

    DEFF Research Database (Denmark)

    Larsen, Lea Lund; Hejlesen, Stine

    2003-01-01

    MI-spillet er et undervisningsspil til folkeskolens mellemtrin og udskolingen. Spillet omformer Howard Gardners teori om de mange intelligenser til et praktisk og håndgribeligt værktøj til brug i folkeskolen. Spillet indeholder et undervisningsmateriale bestående af lærervejledning og kopimappe...... emnebaseret eller tværfagligt arbejde. Alt materialet ligger samlet på en cd-rom, hvorfra materialet printes. Skolen kan derfor ved køb af én cd-rom printe og producere et ubegrænset antal spil. Cd-rommen indeholder: 1. Lærervejledning 2. MI-spillet * Gulvpladerne * Spørgsmål til spillet * Bilag til...

  15. Mi-spillet

    DEFF Research Database (Denmark)

    Larsen, Lea Lund; Hejlesen, Stine

    2003-01-01

    emnebaseret eller tværfagligt arbejde. Alt materialet ligger samlet på en cd-rom, hvorfra materialet printes. Skolen kan derfor ved køb af én cd-rom printe og producere et ubegrænset antal spil. Cd-rommen indeholder: 1. Lærervejledning 2. MI-spillet * Gulvpladerne * Spørgsmål til spillet * Bilag til...

  16. Evaluation of borehole geophysical and video logs, at Butz Landfill Superfund Site, Jackson Township, Monroe County, Pennsylvania

    Science.gov (United States)

    Low, D.J.; Conger, R.W.

    2001-01-01

    Between February 1996 and November 2000, geophysical logging was conducted in 27 open borehole wells in and adjacent to the Butz Landfill Superfund Site, Jackson Township, Monroe County, Pa., to determine casing depth and depths of water-producing zones, water-receiving zones, and zones of vertical borehole flow. The wells range in depth from 57 to 319 feet below land surface. The geophysical logging determined the placement of well screens and packers, which allow monitoring and sampling of water-bearing zones in the fractured bedrock so that the horizontal and vertical distribution of contaminated ground water migrating from known sources could be determined. Geophysical logging included collection of caliper, natural-gamma, single-point-resistance, fluid-resistivity, fluid-temperature, and video logs. Caliper and video logs were used to locate fractures, joints, and weathered zones. Inflections on single-point-resistance, fluid-temperature, and fluid-resistivity logs indicated possible water-bearing fractures, and heatpulse-flowmeter measurements verified these locations. Natural-gamma logs provided information on stratigraphy.

  17. Estonia's defence dollars spent wisely? / Tony Lawrence, Kaarel Kaas ; interv. Joel Alas

    Index Scriptorium Estoniae

    Lawrence, Tony

    2007-01-01

    Rahvusvahelise Kaitseuuringute Keskuse teadurid Tony Lawrence ja Kaarel Kaas kommenteerivad Eestis Suurbritannia kaitseatasheena töötanud kolonelleitnant Glen Granti kriitikat kaitsejõudude efektiivsuse osas, Eesti kaitsepoliitikat, küberrünnakut Eestile, kahe Eesti rahukaitseväelase surma missioonil Afganistanis ning üldsuse suhtumist Eesti osalemisele rahvusvahelistel missioonidel. Lisa: Tony Lawrence; Kaarel Kaas

  18. Estonia's defence dollars spent wisely? / Tony Lawrence, Kaarel Kaas ; interv. Joel Alas

    Index Scriptorium Estoniae

    Lawrence, Tony

    2007-01-01

    Rahvusvahelise Kaitseuuringute Keskuse teadurid Tony Lawrence ja Kaarel Kaas kommenteerivad Eestis Suurbritannia kaitseatasheena töötanud kolonelleitnant Glen Granti kriitikat kaitsejõudude efektiivsuse osas, Eesti kaitsepoliitikat, küberrünnakut Eestile, kahe Eesti rahukaitseväelase surma missioonil Afganistanis ning üldsuse suhtumist Eesti osalemisele rahvusvahelistel missioonidel. Lisa: Tony Lawrence; Kaarel Kaas

  19. Late Quaternary vegetation and glacial history in the St. Lawrence Lowlands, Canada

    NARCIS (Netherlands)

    Lasalle, Pierre

    1966-01-01

    This paper presents data of various kinds concerning the Quaternary geology of the St. Lawrence Lowlands: pollen diagrams, C14 dates, and diatom floras. These data show that the highest parts of the St. Lawrence Lowlands were already deglaciated more than 12,000 years ago, as appears from the existe

  20. Lawrence Kohlberg, una obra en permanente construcción Lawrence Kohlberg, a Work in Permanent Construction

    Directory of Open Access Journals (Sweden)

    Jaime Yánez-Canal

    2012-06-01

    Full Text Available Resumen Este artículo, resultado de investigación documental del grupo Estudios sobre el Desarrollo Sociomoral, presenta un análisis sobre la historia y la evolución intelectual de Lawrece Kohlberg, pionero de la Psicología del Desarrollo Moral. Específicamente, el análisis de su obra gira alrededor de tres tópicos: la filosofía moral, la psicología del desarrollo y la pedagogía.Abstract This paper, documental research outcome of the ''Estudios sobre el Desarrollo Sociomoral'' research group, presents an analysis of the history and intellectual evolution of Lawrence Kohlberg, pioneer of Moral Development Psychology. Specifically, the analysis of his work revolves around three issues: moral Philosophy, developmental Psychology and Pedagogy.

  1. Measurement of the direct <mi>CP> -violating parameter <mi>Ami><mi>CP> in the decay <mi>D>+<mi>Kmi>-<mimi>+<mi>π>+

    Energy Technology Data Exchange (ETDEWEB)

    Abazov, V. M.; Abbott, B.; Acharya, B. S.; Adams, M.; Adams, T.; Agnew, J. P.; Alexeev, G. D.; Alkhazov, G.; Alton, A.; Askew, A.; Atkins, S.; Augsten, K.; Avila, C.; Badaud, F.; Bagby, L.; Baldin, B.; Bandurin, D. V.; Banerjee, S.; Barberis, E.; Baringer, P.; Bartlett, J. F.; Bassler, U.; Bazterra, V.; Bean, A.; Begalli, M.; Bellantoni, L.; Beri, S. B.; Bernardi, G.; Bernhard, R.; Bertram, I.; Besançon, M.; Beuselinck, R.; Bhat, P. C.; Bhatia, S.; Bhatnagar, V.; Blazey, G.; Blessing, S.; Bloom, K.; Boehnlein, A.; Boline, D.; Boos, E. E.; Borissov, G.; Borysova, M.; Brandt, A.; Brandt, O.; Brock, R.; Bross, A.; Brown, D.; Bu, X. B.; Buehler, M.; Buescher, V.; Bunichev, V.; Burdin, S.; Buszello, C. P.; Camacho-Pérez, E.; Casey, B. C. K.; Castilla-Valdez, H.; Caughron, S.; Chakrabarti, S.; Chan, K. M.; Chandra, A.; Chapon, E.; Chen, G.; Cho, S. W.; Choi, S.; Choudhary, B.; Cihangir, S.; Claes, D.; Clutter, J.; Cooke, M.; Cooper, W. E.; Corcoran, M.; Couderc, F.; Cousinou, M. -C.; Cutts, D.; Das, A.; Davies, G.; de Jong, S. J.; De La Cruz-Burelo, E.; Déliot, F.; Demina, R.; Denisov, D.; Denisov, S. P.; Desai, S.; Deterre, C.; DeVaughan, K.; Diehl, H. T.; Diesburg, M.; Ding, P. F.; Dominguez, A.; Dubey, A.; Dudko, L. V.; Duperrin, A.; Dutt, S.; Eads, M.; Edmunds, D.; Ellison, J.; Elvira, V. D.; Enari, Y.; Evans, H.; Evdokimov, V. N.; Fauré, A.; Feng, L.; Ferbel, T.; Fiedler, F.; Filthaut, F.; Fisher, W.; Fisk, H. E.; Fortner, M.; Fox, H.; Fuess, S.; Garbincius, P. H.; Garcia-Bellido, A.; García-González, J. A.; Gavrilov, V.; Geng, W.; Gerber, C. E.; Gershtein, Y.; Ginther, G.; Gogota, O.; Golovanov, G.; Grannis, P. D.; Greder, S.; Greenlee, H.; Grenier, G.; Gris, Ph.; Grivaz, J. -F.; Grohsjean, A.; Grünendahl, S.; Grünewald, M. W.; Guillemin, T.; Gutierrez, G.; Gutierrez, P.; Haley, J.; Han, L.; Harder, K.; Harel, A.; Hauptman, J. M.; Hays, J.; Head, T.; Hebbeker, T.; Hedin, D.; Hegab, H.; Heinson, A. P.; Heintz, U.; Hensel, C.; Heredia-De La Cruz, I.; Herner, K.; Hesketh, G.; Hildreth, M. D.; Hirosky, R.; Hoang, T.; Hobbs, J. D.; Hoeneisen, B.; Hogan, J.; Hohlfeld, M.; Holzbauer, J. L.; Howley, I.; Hubacek, Z.; Hynek, V.; Iashvili, I.; Ilchenko, Y.; Illingworth, R.; Ito, A. S.; Jabeen, S.; Jaffré, M.; Jayasinghe, A.; Jeong, M. S.; Jesik, R.; Jiang, P.; Johns, K.; Johnson, E.; Johnson, M.; Jonckheere, A.; Jonsson, P.; Joshi, J.; Jung, A. W.; Juste, A.; Kajfasz, E.; Karmanov, D.; Katsanos, I.; Kaur, M.; Kehoe, R.; Kermiche, S.; Khalatyan, N.; Khanov, A.; Kharchilava, A.; Kharzheev, Y. N.; Kiselevich, I.; Kohli, J. M.; Kozelov, A. V.; Kraus, J.; Kumar, A.; Kupco, A.; Kurča, T.; Kuzmin, V. A.; Lammers, S.; Lebrun, P.; Lee, H. S.; Lee, S. W.; Lee, W. M.; Lei, X.; Lellouch, J.; Li, D.; Li, H.; Li, L.; Li, Q. Z.; Lim, J. K.; Lincoln, D.; Linnemann, J.; Lipaev, V. V.; Lipton, R.; Liu, H.; Liu, Y.; Lobodenko, A.; Lokajicek, M.; Lopes de Sa, R.; Luna-Garcia, R.; Lyon, A. L.; Maciel, A. K. A.; Madar, R.; Magaña-Villalba, R.; Malik, S.; Malyshev, V. L.; Mansour, J.; Martínez-Ortega, J.; McCarthy, R.; McGivern, C. L.; Meijer, M. M.; Melnitchouk, A.; Menezes, D.; Mercadante, P. G.; Merkin, M.; Meyer, A.; Meyer, J.; Miconi, F.; Mondal, N. K.; Mulhearn, M.; Nagy, E.; Narain, M.; Nayyar, R.; Neal, H. A.; Negret, J. P.; Neustroev, P.; Nguyen, H. T.; Nunnemann, T.; Orduna, J.; Osman, N.; Osta, J.; Pal, A.; Parashar, N.; Parihar, V.; Park, S. K.; Partridge, R.; Parua, N.; Patwa, A.; Penning, B.; Perfilov, M.; Peters, Y.; Petridis, K.; Petrillo, G.; Pétroff, P.; Pleier, M. -A.; Podstavkov, V. M.; Popov, A. V.; Prewitt, M.; Price, D.; Prokopenko, N.; Qian, J.; Quadt, A.; Quinn, B.; Ratoff, P. N.; Razumov, I.; Ripp-Baudot, I.; Rizatdinova, F.; Rominsky, M.; Ross, A.; Royon, C.; Rubinov, P.; Ruchti, R.; Sajot, G.; Sánchez-Hernández, A.; Sanders, M. P.; Santos, A. S.; Savage, G.; Savitskyi, M.; Sawyer, L.; Scanlon, T.; Schamberger, R. D.; Scheglov, Y.; Schellman, H.; Schwanenberger, C.; Schwienhorst, R.; Sekaric, J.; Severini, H.; Shabalina, E.; Shary, V.; Shaw, S.; Shchukin, A. A.; Simak, V.; Skubic, P.; Slattery, P.; Smirnov, D.; Snow, G. R.; Snow, J.; Snyder, S.; Söldner-Rembold, S.; Sonnenschein, L.; Soustruznik, K.; Stark, J.; Stoyanova, D. A.; Strauss, M.; Suter, L.; Svoisky, P.; Titov, M.; Tokmenin, V. V.; Tsai, Y. -T.; Tsybychev, D.; Tuchming, B.; Tully, C.; Uvarov, L.; Uvarov, S.; Uzunyan, S.; Van Kooten, R.; van Leeuwen, W. M.; Varelas, N.; Varnes, E. W.; Vasilyev, I. A.; Verkheev, A. Y.; Vertogradov, L. S.; Verzocchi, M.; Vesterinen, M.; Vilanova, D.; Vokac, P.; Wahl, H. D.; Wang, M. H. L. S.; Warchol, J.; Watts, G.; Wayne, M.; Weichert, J.; Welty-Rieger, L.; Williams, M. R. J.; Wilson, G. W.; Wobisch, M.; Wood, D. R.; Wyatt, T. R.; Xie, Y.; Yamada, R.; Yang, S.; Yasuda, T.; Yatsunenko, Y. A.; Ye, W.; Ye, Z.; Yin, H.; Yip, K.; Youn, S. W.; Yu, J. M.; Zennamo, J.; Zhao, T. G.; Zhou, B.; Zhu, J.; Zielinski, M.; Zieminska, D.; Zivkovic, L.

    2014-12-01

    We measure the direct mi>Cmi>mi>P>-violating parameter mi>Ami>mi>Cmi>mi>Pmi> for the decay of the charged charm meson, mi>Dmi>+mi>Kmi>-mi>πmi>+mi>πmi>+ (and charge conjugate), using the full 10.4 mi>fbmi>-1 sample of mi>p>mi>p>¯ collisions at mi>smi>=1.96 mi>TeVmi> collected by the D0 detector at the Fermilab Tevatron collider. We extract the raw reconstructed charge asymmetry by fitting the invariant mass distributions for the sum and difference of charge-specific samples. This quantity is then corrected for detector-related asymmetries using data-driven methods and for possible physics asymmetries (from mi>B>mi>D

  2. Measurement of the direct <mi>CP> -violating parameter <mi>Ami><mi>CP> in the decay <mi>D>+<mi>Kmi>-<mimi>+<mi>π>+

    Energy Technology Data Exchange (ETDEWEB)

    Abazov, V. M.; Abbott, B.; Acharya, B. S.; Adams, M.; Adams, T.; Agnew, J. P.; Alexeev, G. D.; Alkhazov, G.; Alton, A.; Askew, A.; Atkins, S.; Augsten, K.; Avila, C.; Badaud, F.; Bagby, L.; Baldin, B.; Bandurin, D. V.; Banerjee, S.; Barberis, E.; Baringer, P.; Bartlett, J. F.; Bassler, U.; Bazterra, V.; Bean, A.; Begalli, M.; Bellantoni, L.; Beri, S. B.; Bernardi, G.; Bernhard, R.; Bertram, I.; Besançon, M.; Beuselinck, R.; Bhat, P. C.; Bhatia, S.; Bhatnagar, V.; Blazey, G.; Blessing, S.; Bloom, K.; Boehnlein, A.; Boline, D.; Boos, E. E.; Borissov, G.; Borysova, M.; Brandt, A.; Brandt, O.; Brock, R.; Bross, A.; Brown, D.; Bu, X. B.; Buehler, M.; Buescher, V.; Bunichev, V.; Burdin, S.; Buszello, C. P.; Camacho-Pérez, E.; Casey, B. C. K.; Castilla-Valdez, H.; Caughron, S.; Chakrabarti, S.; Chan, K. M.; Chandra, A.; Chapon, E.; Chen, G.; Cho, S. W.; Choi, S.; Choudhary, B.; Cihangir, S.; Claes, D.; Clutter, J.; Cooke, M.; Cooper, W. E.; Corcoran, M.; Couderc, F.; Cousinou, M. -C.; Cutts, D.; Das, A.; Davies, G.; de Jong, S. J.; De La Cruz-Burelo, E.; Déliot, F.; Demina, R.; Denisov, D.; Denisov, S. P.; Desai, S.; Deterre, C.; DeVaughan, K.; Diehl, H. T.; Diesburg, M.; Ding, P. F.; Dominguez, A.; Dubey, A.; Dudko, L. V.; Duperrin, A.; Dutt, S.; Eads, M.; Edmunds, D.; Ellison, J.; Elvira, V. D.; Enari, Y.; Evans, H.; Evdokimov, V. N.; Fauré, A.; Feng, L.; Ferbel, T.; Fiedler, F.; Filthaut, F.; Fisher, W.; Fisk, H. E.; Fortner, M.; Fox, H.; Fuess, S.; Garbincius, P. H.; Garcia-Bellido, A.; García-González, J. A.; Gavrilov, V.; Geng, W.; Gerber, C. E.; Gershtein, Y.; Ginther, G.; Gogota, O.; Golovanov, G.; Grannis, P. D.; Greder, S.; Greenlee, H.; Grenier, G.; Gris, Ph.; Grivaz, J. -F.; Grohsjean, A.; Grünendahl, S.; Grünewald, M. W.; Guillemin, T.; Gutierrez, G.; Gutierrez, P.; Haley, J.; Han, L.; Harder, K.; Harel, A.; Hauptman, J. M.; Hays, J.; Head, T.; Hebbeker, T.; Hedin, D.; Hegab, H.; Heinson, A. P.; Heintz, U.; Hensel, C.; Heredia-De La Cruz, I.; Herner, K.; Hesketh, G.; Hildreth, M. D.; Hirosky, R.; Hoang, T.; Hobbs, J. D.; Hoeneisen, B.; Hogan, J.; Hohlfeld, M.; Holzbauer, J. L.; Howley, I.; Hubacek, Z.; Hynek, V.; Iashvili, I.; Ilchenko, Y.; Illingworth, R.; Ito, A. S.; Jabeen, S.; Jaffré, M.; Jayasinghe, A.; Jeong, M. S.; Jesik, R.; Jiang, P.; Johns, K.; Johnson, E.; Johnson, M.; Jonckheere, A.; Jonsson, P.; Joshi, J.; Jung, A. W.; Juste, A.; Kajfasz, E.; Karmanov, D.; Katsanos, I.; Kaur, M.; Kehoe, R.; Kermiche, S.; Khalatyan, N.; Khanov, A.; Kharchilava, A.; Kharzheev, Y. N.; Kiselevich, I.; Kohli, J. M.; Kozelov, A. V.; Kraus, J.; Kumar, A.; Kupco, A.; Kurča, T.; Kuzmin, V. A.; Lammers, S.; Lebrun, P.; Lee, H. S.; Lee, S. W.; Lee, W. M.; Lei, X.; Lellouch, J.; Li, D.; Li, H.; Li, L.; Li, Q. Z.; Lim, J. K.; Lincoln, D.; Linnemann, J.; Lipaev, V. V.; Lipton, R.; Liu, H.; Liu, Y.; Lobodenko, A.; Lokajicek, M.; Lopes de Sa, R.; Luna-Garcia, R.; Lyon, A. L.; Maciel, A. K. A.; Madar, R.; Magaña-Villalba, R.; Malik, S.; Malyshev, V. L.; Mansour, J.; Martínez-Ortega, J.; McCarthy, R.; McGivern, C. L.; Meijer, M. M.; Melnitchouk, A.; Menezes, D.; Mercadante, P. G.; Merkin, M.; Meyer, A.; Meyer, J.; Miconi, F.; Mondal, N. K.; Mulhearn, M.; Nagy, E.; Narain, M.; Nayyar, R.; Neal, H. A.; Negret, J. P.; Neustroev, P.; Nguyen, H. T.; Nunnemann, T.; Orduna, J.; Osman, N.; Osta, J.; Pal, A.; Parashar, N.; Parihar, V.; Park, S. K.; Partridge, R.; Parua, N.; Patwa, A.; Penning, B.; Perfilov, M.; Peters, Y.; Petridis, K.; Petrillo, G.; Pétroff, P.; Pleier, M. -A.; Podstavkov, V. M.; Popov, A. V.; Prewitt, M.; Price, D.; Prokopenko, N.; Qian, J.; Quadt, A.; Quinn, B.; Ratoff, P. N.; Razumov, I.; Ripp-Baudot, I.; Rizatdinova, F.; Rominsky, M.; Ross, A.; Royon, C.; Rubinov, P.; Ruchti, R.; Sajot, G.; Sánchez-Hernández, A.; Sanders, M. P.; Santos, A. S.; Savage, G.; Savitskyi, M.; Sawyer, L.; Scanlon, T.; Schamberger, R. D.; Scheglov, Y.; Schellman, H.; Schwanenberger, C.; Schwienhorst, R.; Sekaric, J.; Severini, H.; Shabalina, E.; Shary, V.; Shaw, S.; Shchukin, A. A.; Simak, V.; Skubic, P.; Slattery, P.; Smirnov, D.; Snow, G. R.; Snow, J.; Snyder, S.; Söldner-Rembold, S.; Sonnenschein, L.; Soustruznik, K.; Stark, J.; Stoyanova, D. A.; Strauss, M.; Suter, L.; Svoisky, P.; Titov, M.; Tokmenin, V. V.; Tsai, Y. -T.; Tsybychev, D.; Tuchming, B.; Tully, C.; Uvarov, L.; Uvarov, S.; Uzunyan, S.; Van Kooten, R.; van Leeuwen, W. M.; Varelas, N.; Varnes, E. W.; Vasilyev, I. A.; Verkheev, A. Y.; Vertogradov, L. S.; Verzocchi, M.; Vesterinen, M.; Vilanova, D.; Vokac, P.; Wahl, H. D.; Wang, M. H. L. S.; Warchol, J.; Watts, G.; Wayne, M.; Weichert, J.; Welty-Rieger, L.; Williams, M. R. J.; Wilson, G. W.; Wobisch, M.; Wood, D. R.; Wyatt, T. R.; Xie, Y.; Yamada, R.; Yang, S.; Yasuda, T.; Yatsunenko, Y. A.; Ye, W.; Ye, Z.

    2014-12-01

    We measure the direct mi>Cmi>mi>P>-violating parameter mi>Ami>mi>Cmi>mi>Pmi> for the decay of the charged charm meson, mi>Dmi>+mi>Kmi>-mi>πmi>+mi>πmi>+ (and charge conjugate), using the full 10.4 mi>fbmi>-1 sample of mi>p>mi>p>¯ collisions at mi>smi>=1.96 mi>TeVmi> collected by the D0 detector at the Fermilab Tevatron collider. We extract the raw reconstructed charge asymmetry by fitting the invariant mass distributions for the sum and difference of charge-specific samples. This quantity is then corrected for detector-related asymmetries using data-driven methods and for possible physics asymmetries (from mi>B>mi>D

  3. Berkeley Lab's Saul Perlmutter wins E.O. Lawrence Award; scientist's work on supernovae reveals accelerating Universe

    CERN Multimedia

    2002-01-01

    Saul Perlmutter, from Lawrence Berkeley National Laboratory Physics Division and leader of the Supernova Cosmology Project based there, has won the DOE's 2002 E.O. Lawrence Award in the physics category (2 pages).

  4. MiDAS

    DEFF Research Database (Denmark)

    McIlroy, Simon Jon; Kirkegaard, Rasmus Hansen; McIlroy, Bianca

    A deep understanding of the microbial communities and dynamics in wastewater treatment systems is a powerful tool for process optimization and design (Rittmann et al., 2006). With the advent of amplicon sequencing of the 16S rRNA gene, the diversity within the microbial communities can now...... web platform about the microbes in activated sludge and their associated ADs. The MiDAS taxonomy proposes putative names for each genus-level-taxon that can be used as a common vocabulary for all researchers in the field....

  5. Lawrence Livermore National Laboratory environmental report for 1990

    Energy Technology Data Exchange (ETDEWEB)

    Sims, J.M.; Surano, K.A.; Lamson, K.C.; Balke, B.K.; Steenhoven, J.C.; Schwoegler, D.R. (eds.)

    1990-01-01

    This report documents the results of the Environmental Monitoring Program at the Lawrence Livermore National Laboratory (LLNL) and presents summary information about environmental compliance for 1990. To evaluate the effect of LLNL operations on the local environment, measurements of direct radiation and a variety of radionuclides and chemical compounds in ambient air, soil, sewage effluent surface water, groundwater, vegetation, and foodstuff were made at both the Livermore site and at Site 300 nearly. LLNL's compliance with all applicable guides, standards, and limits for radiological and nonradiological emissions to the environment was evaluated. Aside from an August 13 observation of silver concentrations slightly above guidelines for discharges to the sanitary sewer, all the monitoring data demonstrated LLNL compliance with environmental laws and regulations governing emission and discharge of materials to the environment. In addition, the monitoring data demonstrated that the environmental impacts of LLNL are minimal and pose no threat to the public to or to the environment. 114 refs., 46 figs., 79 tabs.

  6. Environmental Survey preliminary report, Lawrence Livermore National Laboratory, Livermore, California

    Energy Technology Data Exchange (ETDEWEB)

    1987-12-01

    This report presents the preliminary findings from the first phase of the Environmental Survey of the Department of Energy (DOE) Lawrence Livermore National Laboratory (LLNL), conducted December 1 through 19, 1986. The Survey is being conducted by an interdisciplinary team of environmental specialists, led and managed by the Office of Environment, Safety and Health's Office of Environmental Audit. Individual team components are being supplied by a private contractor. The objective of the Survey is to identify environmental problems and areas of environmental risk associated with LLNL. The Survey covers all environmental media all areas of environmental regulation. It is being performed in accordance with the DOE Environmental Survey Manual. This phase of the Survey involves the review of existing site environmental data, observations of the operations performed at LLNL, and interviews with site personnel. A Sampling and Analysis Plan was developed to assist in further assessing certain of the environmental problems identified during performance of on-site activities. The Sampling and Analysis Plan will be executed by a DOE National Laboratory. When completed, the results will be incorporated into the LLNL Environmental Survey Interim Report. The Interim Report will reflect the final determinations of the LLNL Survey. 70 refs., 58 figs., 52 tabs.,

  7. Environmental Survey preliminary report, Lawrence Berkeley Laboratory, Berkeley, California

    Energy Technology Data Exchange (ETDEWEB)

    1988-07-01

    The purpose of this report is to present the preliminary findings made during the Environmental Survey, February 22--29, 1988, at the US Department of Energy (DOE) Lawrence Berkeley Laboratory (LBL) in Berkeley, California. The University of California operates the LBL facility for DOE. The LBL Survey is part of the larger DOE-wide Environmental Survey announced by Secretary John S. Herrington on September 18, 1985. The purpose of this effort is to identify, via no fault'' baseline Surveys, existing environmental problems and areas of environmental risk at DOE facilities, and to rank them on a DOE wide basis. This ranking will enable DOE to more effectively establish priorities for addressing environmental problems and allocate the resources necessary to correct them. Because the Survey is no fault'' and is not an audit,'' it is not designed to identify specific isolated incidents of noncompliance or to analyze environmental management practices. Such incidents and/or management practices will, however, be used in the Survey as a means of identifying existing and potential environmental problems. The LBL Survey was conducted by a multidisciplinary team of technical specialists headed and managed by a Team Leader and Assistant Team Leader from DOE's Office of Environmental Audit. A complete list of the LBL Survey participants and their affiliations is provided in Appendix A. 80 refs., 27 figs., 37 tabs.

  8. Waste characterization activities at the Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Roberson, G.P.; Martz, H.E.; Haskins, J.J. [and others

    1995-06-28

    Radioactive and hazardous wastes are generated at many national laboratories, military sites, fuel fabrication and enrichment plants, reactors, and many other facilities. At all of these sites, wastes must be separated, categorized, possibly treated, and packed into containers for shipment to waste-storage or disposal sites. Prior to treatment, storage or, shipment, the containers must be characterized to determine the ultimate disposition of the contained waste. Comprehensive and accurate nondestructive evaluation (NDE) and nondestructive assay (NDA) methods can be used to characterize most waste containers in a safe and cost-effective manner without opening them. The Lawrence Livermore National Laboratory (LLNL) is investigating and developing the application of x-ray and {gamma}-ray methods to nonintrusively characterize waste containers and/or items. X-ray NDE methods are being investigated to determine whether they can be used to identify hazardous and nonconforming materials. A {gamma}-ray NDA method is used to identify the radioactive sources within a container and to accurately quantify their strength. In this paper we describe five waste characterization projects being conducted at LLNL that apply both the NDE and NDA methods and present results.

  9. Lawrence Livermore National Laboratory Working Reference Material Production Pla

    Energy Technology Data Exchange (ETDEWEB)

    Wong, Amy; Thronas, Denise; Marshall, Robert

    1998-11-04

    This Lawrence Livermore National Laboratory (LLNL) Working Reference Material Production Plan was written for LLNL by the Los Alamos National Laboratory to address key elements of producing seven Pu-diatomaceous earth NDA Working Reference Materials (WRMS). These WRMS contain low burnup Pu ranging in mass from 0.1 grams to 68 grams. The composite Pu mass of the seven WRMS was designed to approximate the maximum TRU allowable loading of 200 grams Pu. This document serves two purposes: first, it defines all the operations required to meet the LLNL Statement of Work quality objectives, and second, it provides a record of the production and certification of the WRMS. Guidance provided in ASTM Standard Guide C1128-89 was used to ensure that this Plan addressed all the required elements for producing and certifying Working Reference Materials. The Production Plan was written to provide a general description of the processes, steps, files, quality control, and certification measures that were taken to produce the WRMS. The Plan identifies the files where detailed procedures, data, quality control, and certification documentation and forms are retained. The Production Plan is organized into three parts: a) an initial section describing the preparation and characterization of the Pu02 and diatomaceous earth materials, b) middle sections describing the loading, encapsulation, and measurement on the encapsulated WRMS, and c) final sections describing the calculations of the Pu, Am, and alpha activity for the WRMS and the uncertainties associated with these quantities.

  10. Tiger Team assessment of the Lawrence Berkeley Laboratory, Washington, DC

    Energy Technology Data Exchange (ETDEWEB)

    1991-02-01

    This report documents the results of the Department of Energy's (DOE's) Tiger Team Assessment of the Lawrence Berkeley Laboratory (LBL) conducted from January 14 through February 15, 1991. The purpose of the assessment was to provide the Secretary of Energy with the status of environment, safety, and health (ES H) programs at LBL. The Tiger Team concluded that curtailment of cessation of any operations at LBL is not warranted. However, the number and breadth of findings and concerns from this assessment reflect a serious condition at this site. In spite of its late start, LBL has recently made progress in increasing ES H awareness at all staff levels and in identifying ES H deficiencies. Corrective action plans are inadequate, however, many compensatory actions are underway. Also, LBL does not have the technical expertise or training programs nor the tracking and followup to effectively direct and control sitewide guidance and oversight by DOE of ES H activities at LBL. As a result of these deficiencies, the Tiger Team has reservations about LBL's ability to implement effective actions in a timely manner and, thereby, achieve excellence in their ES H program. 4 figs., 24 tabs.

  11. Comparison of St. Lawrence blue whale vocalizations with field observations

    Science.gov (United States)

    Berchok, Catherine; Bradley, David; Gabrielson, Thomas; Sears, Richard

    2003-04-01

    During four field seasons from 1998-2001, vocalizations were recorded in the presence of St. Lawrence blue whales using a single omni-directional hydrophone. Both long duration infrasonic calls (~18 Hz, 5-20 s) as well as short duration higher frequency calls (85-25 Hz, ~2 s) were detected and compared with field observations. Two trends were noted. First, the long infrasonic call series were concentrated primarily in the deep (300 m) channel. These call series appear to compare well with blue whale vocalizations recorded by others in the deep open ocean. Second, the shorter audible calls were more evenly distributed over bathymetry and seem to be a form of short distance communication with at least one case occurring during an agonistic interaction. A comparison of these calls with biological parameters such as density of whales in the area, percentages of paired versus single whales, and numbers of males versus females will also be discussed. [Project supported by ARL/PSU, NSF, and the American Museum of Natural History.

  12. Lawrence Berkeley National Laboratory 2015 Annual Financial Report

    Energy Technology Data Exchange (ETDEWEB)

    Williams, Kim, P

    2017-08-11

    FY2015 financial results reflect a year of significant scientific, operational and financial achievement for Lawrence Berkeley National Laboratory. Complementing many scientific accomplishments, Berkeley Lab completed construction of four new research facilities: the General Purpose Laboratory, Chu Hall, Wang Hall and the Flexlab Building Efficiency Testbed. These state-of-the-art facilities allow for program growth and enhanced collaboration, in part by enabling programs to return to the Lab’s Hill Campus from offsite locations. Detailed planning began for the new Integrative Genomics Building (IGB) that will house another major program currently located offsite. Existing site infrastructure was another key focus area. The Lab prioritized and increased investments in deferred maintenance in alignment with the Berkeley Lab Infrastructure Plan, which was developed under the leadership of the DOE Office of Science. With the expiration of American Recovery and Reinvestment Act (ARRA) funds, we completed the close-out of all of our 134 ARRA projects, recording total costs of $331M over the FY2009-2015 period. Download the report to read more.

  13. 2020 Foresight Forging the Future of Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Chrzanowski, P.

    2000-01-01

    The Lawrence Livermore National Laboratory (LLNL) of 2020 will look much different from the LLNL of today and vastly different from how it looked twenty years ago. We, the members of the Long-Range Strategy Project, envision a Laboratory not defined by one program--nuclear weapons research--but by several core programs related to or synergistic with LLNL's national security mission. We expect the Laboratory to be fully engaged with sponsors and the local community and closely partnering with other research and development (R&D) organizations and academia. Unclassified work will be a vital part of the Laboratory of 2020 and will visibly demonstrate LLNL's international science and technology strengths. We firmly believe that there will be a critical and continuing role for the Laboratory. As a dynamic and versatile multipurpose laboratory with a national security focus, LLNL will be applying its capabilities in science and technology to meet the needs of the nation in the 21st century. With strategic investments in science, outstanding technical capabilities, and effective relationships, the Laboratory will, we believe, continue to play a key role in securing the nation's future.

  14. STS-114 Crew Interviews: 1. Eileen Collins 2. Wendy Lawrence

    Science.gov (United States)

    2005-01-01

    1) STS-114 Commander Eileen Collins emphasized her love for teaching, respect for teachers, and her plan to go back to teaching again someday. Her solid background in Math and Science, focus on her interests, with great support from her family, and great training and support during her career with the Air Force gave her confidence in pursuing her dream to become an astronaut. Commander Collins shares her thoughts on the Columbia, details the various flight operations and crew tasks that will take place during the mission and the importance of Shuttle missions to the International Space Station and space exploration. 2) STS-114 Mission Specialist Wendy Lawrence first dreamed of becoming an astronaut when she watched Neil Armstrong walk on the moon from their black and white TV set. She majored in Engineering and became a Navy pilot. She shares her thoughts on the Columbia, details her major role as the crew in charge of all the transfer operations; getting the MPLM unpacked and repacked; and the importance of Shuttle missions to the International Space Station and space exploration.

  15. Hydraulic Evaluation of Culvert Valves at Eisenhower and Snell Locks, St. Lawrence Seaway

    Science.gov (United States)

    2015-06-01

    ER D C/ CH L TR -1 5- 7 Hydraulic Evaluation of Culvert Valves at Eisenhower and Snell Locks, St. Lawrence Seaway Co as ta l a nd H...client/default. ERDC/CHL TR-15-7 June 2015 Hydraulic Evaluation of Culvert Valves at Eisenhower and Snell Locks, St. Lawrence Seaway...ERDC/CHL TR-15-7 ii Abstract The aged, double-skin-plate valves of the Eisenhower and Snell Locks on the St. Lawrence Seaway are being replaced

  16. Beyond Lawrence v. Texas: crafting a fundamental right to sexual privacy.

    Science.gov (United States)

    Fasullo, Kristin

    2009-05-01

    After the watershed 2003 U.S. Supreme Court decision Lawrence v.Texas, courts are faced with the daunting task of navigating the bounds of sexual privacy in light of Lawrence's sweeping language and unconventional structure. This Note focuses on the specific issue of state governments regulating sexual device distribution. Evaluating the substantive due process rights of sexual device retailers and users, this Note ultimately argues that the privacy interest identified in Lawrence is sufficiently broad to protect intimate decisions to engage in adult consensual sexual behavior, including the liberty to sell, purchase, and use a sexual device.

  17. THE CONTRIBUTION MADE BY T.E. LAWRENCE TO THE THEORY OF REVOLUTIONARY WARFARE

    Directory of Open Access Journals (Sweden)

    G.K.B. Barron

    2012-02-01

    Full Text Available Lawrence was basically an academic thrown into the hurly-burly of leading an Arab revolt against Turkish domination. It could be said that the war in the Middle East was a sideshow of the First World War and Lawrence's part was a ' ... sideshow to the sideshow'l Why then has Lawrence been remembered when greater military men have been forgotten? The romanticism of his exploits are surely the reason, and yet the fact that he is the first modern theorist and possibly the "father" of modern revolutionary warfare, tends to be forgotten.

  18. "A Prison for the Infinite": D. H. Lawrence and Bertrand Russell on the War

    OpenAIRE

    Ferretter, Luke

    2015-01-01

    In his recent book War Trauma and English Modernism, Carl Krockel argues that Lawrence suffered from "war trauma," throughout not only the war years but for almost the entire remainder of his life. He is right to say so. The war smashed Lawrence, as an artist and as a man, and I would disagree with Krockel's thesis only insofar as he sees the beginning of a healing process at work in the final draft of Lady Chatterley's Lover (153-4). Lawrence responded to the war in many ways at the time – t...

  19. Impact of the January-February 1980 earthquake sequence on various structures at the Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Murray, R.C.; Nelson, T.A.; Coats, D.W.; Ng, D.S.; Weaver, H.J.

    1981-02-04

    On January 24, 1980, California's Livermore Valley was rocked by a moderate earthquake that caused some damage to the Lawrence Livermore National Laboratory (LLNL). The earthquake, which measured 5.5 on the Richter scale and was centered about 20 km (12 mi) northwest of the Laboratory, produced estimated peak horizontal ground acceleration at LLNL of between 0.15 and 0.3 g. The earthquake was part of a sequence that included two sharp aftershocks (magnitudes 5.2 and 4.2) within 1.5 minutes of the initial event. A second earthquake (magnitude 5.8) struck on January 26, and several lesser earthquakes occurred during the next few weeks. This paper describes the damage impact of the January 24 earthquake, including: background information on LLNL, discussion of pre-earthquake seismic safety philosophy, and description of the impact of the January 24 earthquake, including a description of the seismic setting of the Laboratory, a discussion of the ground motion, and a summary of damage. This paper also describes a data gathering and reduction effort at LLNL in the aftermath of the January earthquakes.

  20. Role of pri-miRNA tertiary structure in miR-17~92 miRNA biogenesis.

    Science.gov (United States)

    Chaulk, Steven G; Thede, Gina L; Kent, Oliver A; Xu, Zhizhong; Gesner, Emily M; Veldhoen, Richard A; Khanna, Suneil K; Goping, Ing Swie; MacMillan, Andrew M; Mendell, Joshua T; Young, Howard S; Fahlman, Richard P; Glover, J N Mark

    2011-01-01

    MicroRNAs (miRNAs) regulate gene expression in a variety of biological pathways such as development and tumourigenesis. miRNAs are initially expressed as long primary transcripts (pri-miRNAs) that undergo sequential processing by Drosha and then Dicer to yield mature miRNAs. miR-17~92 is a miRNA cluster that encodes 6 miRNAs and while it is essential for development it also has reported oncogenic activity. To date, the role of RNA structure in miRNA biogenesis has only been considered in terms of the secondary structural elements required for processing of pri-miRNAs by Drosha. Here we report that the miR-17~92 cluster has a compact globular tertiary structure where miRNAs internalized within the core of the folded structure are processed less efficiently than miRNAs on the surface of the structure. Increased miR-92 expression resulting from disruption of the compact miR-17~92 structure results in increased repression of integrin α5 mRNA, a known target of miR-92a. In summary, we describe the first example of pri-miRNA structure modulating differential expression of constituent miRNAs.

  1. Processing Gulf of St. Lawrence pink shrimps at Eagle Fisheries Co. Ltd., Shippegan, N.B

    National Research Council Canada - National Science Library

    Gionet, W

    1969-01-01

    ... in the development of a shrimp fishery during the 1968 season in the Gulf of St. Lawrence. Specialized equipment necessary for catching and holding shrimp as well as for processing was designed and constructed...

  2. St. Lawrence blue whale vocalizations revisited: characterization of calls detected from 1998 to 2001.

    Science.gov (United States)

    Berchok, Catherine L; Bradley, David L; Gabrielson, Thomas B

    2006-10-01

    From 1998 to 2001, 115 h of acoustic recordings were made in the presence of the well-studied St. Lawrence population of blue whales, using a calibrated omnidirectional hydrophone [flat (+/- 3 dB) response from 5 to 800 Hz] suspended at 50 m depth from a surface isolation buoy. The primary field site for this study was the estuary region of the St. Lawrence River (Québec, Canada), with most recordings made between mid-August and late October. During the recordings, detailed field notes were taken on all cetaceans within sight. Characterization of the more than 1000 blue whale calls detected during this study revealed that the St. Lawrence repertoire is much more extensive than previously reported. Three infrasonic (whales. Although St. Lawrence blue whale call characteristics are similar to those of the North Atlantic, comparisons of phrase composition and spacing among studies suggest the possibility of population dialects within the North Atlantic.

  3. Polygons Representing Sensitivity of Ground Water to Contamination in Lawrence County, SD

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This data set includes 956 polygons labeled with a sensitivity-unit code that represents the sensitivity of ground water to contamination in Lawrence County, SD....

  4. Lawrence Berkeley Laboratory, Institutional Plan FY 1994--1999

    Energy Technology Data Exchange (ETDEWEB)

    1993-09-01

    The Institutional Plan provides an overview of the Lawrence Berkeley Laboratory mission, strategic plan, scientific initiatives, research programs, environment and safety program plans, educational and technology transfer efforts, human resources, and facilities needs. For FY 1994-1999 the Institutional Plan reflects significant revisions based on the Laboratory`s strategic planning process. The Strategic Plan section identifies long-range conditions that will influence the Laboratory, as well as potential research trends and management implications. The Initiatives section identifies potential new research programs that represent major long-term opportunities for the Laboratory, and the resources required for their implementation. The Scientific and Technical Programs section summarizes current programs and potential changes in research program activity. The Environment, Safety, and Health section describes the management systems and programs underway at the Laboratory to protect the environment, the public, and the employees. The Technology Transfer and Education programs section describes current and planned programs to enhance the nation`s scientific literacy and human infrastructure and to improve economic competitiveness. The Human Resources section identifies LBL staff diversity and development program. The section on Site and Facilities discusses resources required to sustain and improve the physical plant and its equipment. The new section on Information Resources reflects the importance of computing and communication resources to the Laboratory. The Resource Projections are estimates of required budgetary authority for the Laboratory`s ongoing research programs. The Institutional Plan is a management report for integration with the Department of Energy`s strategic planning activities, developed through an annual planning process.

  5. Community Relations Plan for Lawrence Berkeley Laboratory. Environmental Restoration Program

    Energy Technology Data Exchange (ETDEWEB)

    1993-07-01

    The Lawrence Berkeley Laboratory (LBL) has applied to the California Environmental Protection Agency, Department of Toxic Substances Control (DTSC), for renewal of its Hazardous Waste Handling Facility Permit. A permit is required under Resource Conservation and Recovery Act (RCRA) regulations. The permit will allow LBL to continue using its current hazardous waste handling facility, upgrade the existing facility, and construct a replacement facility. The new facility is scheduled for completion in 1995. The existing facility will be closed under RCRA guidelines by 1996. As part of the permitting process, LBL is required to investigate areas of soil and groundwater contamination at its main site in the Berkeley Hills. The investigations are being conducted by LBL`s Environmental Restoration Program and are overseen by a number of regulatory agencies. The regulatory agencies working with LBL include the California Environmental Protection Agency`s Department of Toxic Substances Control, the California Regional Water Quality Control Board, the Bay Area Air Quality Management District, the East Bay Municipal Utilities District, and the Berkeley Department of Environmental Health. RCRA requires that the public be informed of LBL`s investigations and site cleanup, and that opportunities be available for the public to participate in making decisions about how LBL will address contamination issues. LBL has prepared this Community Relations Plan (CRP) to describe activities that LBL will use to keep the community informed of environmental restoration progress and to provide for an open dialogue with the public on issues of importance. The CRP documents the community`s current concerns about LBL`s Environmental Restoration Program. Interviews conducted between February and April 1993 with elected officials, agency staff, environmental organizations, businesses, site neighbors, and LBL employees form the basis for the information contained in this document.

  6. Lawrence Berkeley Laboratory Institutional Plan, FY 1993--1998

    Energy Technology Data Exchange (ETDEWEB)

    1992-10-01

    The FY 1993--1998 Institutional Plan provides an overview of the Lawrence Berkeley Laboratory mission, strategic plan, scientific initiatives, research programs, environment and safety program plans, educational and technology transfer efforts, human resources, and facilities needs. The Strategic Plan section identifies long-range conditions that can influence the Laboratory, potential research trends, and several management implications. The Initiatives section identifies potential new research programs that represent major long-term opportunities for the Laboratory and the resources required for their implementation. The Scientific and Technical Programs section summarizes current programs and potential changes in research program activity. The Environment, Safety, and Health section describes the management systems and programs underway at the Laboratory to protect the environment, the public, and the employees. The Technology Transfer and Education programs section describes current and planned programs to enhance the nation's scientific literacy and human infrastructure and to improve economic competitiveness. The Human Resources section identifies LBL staff composition and development programs. The section on Site and Facilities discusses resources required to sustain and improve the physical plant and its equipment. The Resource Projections are estimates of required budgetary authority for the Laboratory's ongoing research programs. The plan is an institutional management report for integration with the Department of Energy's strategic planning activities that is developed through an annual planning process. The plan identifies technical and administrative directions in the context of the National Energy Strategy and the Department of Energy's program planning initiatives. Preparation of the plan is coordinated by the Office for Planning and Development from information contributed by the Laboratory's scientific and support divisions.

  7. Topectomy versus leukotomy: J. Lawrence Pool's contribution to psychosurgery.

    Science.gov (United States)

    Holland, Ryan; Kopel, David; Carmel, Peter W; Prestigiacomo, Charles J

    2017-09-01

    Surgery of the mind has a rather checkered past. Though its history begins with the prehistoric trephination of skulls to allow "evil spirits" to escape, the early- to mid-20th century saw a surge in the popularity of psychosurgery. The 2 prevailing operations were topectomy and leukotomy for the treatment of certain mental illnesses. Although they were modified and refined by several of their main practitioners, the effectiveness of and the ethics involved with these operations remained controversial. In 1947, Dr. J. Lawrence Pool and the Columbia-Greystone Associates sought to rigorously investigate the outcomes of specific psychosurgical procedures. Pool along with R. G. Heath and John Weber believed that nonexcessive bifrontal cortical ablation could successfully treat certain mental illnesses without the undesired consequences of irreversible personality changes. They conducted this investigation at the psychiatric hospital at Greystone Park near Morristown, New Jersey. Despite several encouraging findings of the Columbia-Greystone project, psychosurgery practices began to decline significantly in the 1950s. The uncertainty of results and ethical debates related to side effects made these procedures unpopular. Further, groups such as the National Association for the Advancement of Colored People and the American Civil Liberties Union condemned the use of psychosurgery, believing it to be an inhumane form of treatment. Today, there are strict guidelines that must be adhered to when evaluating a patient for psychosurgery procedures. It is imperative for the neurosurgery community to remember the history of psychosurgery to provide the best possible current treatment and to search for better future treatments for a particularly vulnerable patient population.

  8. Status of the belugas of the St Lawrence estuary, Canada

    Directory of Open Access Journals (Sweden)

    Michael CS Kingsley

    2002-07-01

    Full Text Available A population of belugas (Delphinapterus leucas inhabiting the estuary of the St Lawrence river in Quebec, Canada, was depleted by unregulated hunting, not closed until 1979. Surveys in 1977 showed only a few hundred in the population. Surveys since then have produced increasing estimates of population indices. An estimate of the population, fully corrected for diving animals, was 1,238 (SE 119 in September 1997. The population was estimated to have increased from 1988 through 1997 by 31.4 belugas/yr (SE 13.1. Observations of population age structure, as well as data on age at death obtained from beach-cast carcasses, do not indicate serious problems at the population level, although there are indications that mortality of the oldest animals may be elevated. Few animals appear to live much over 30 years. From examination of beach-cast carcasses, it appears that most deaths are due to old age and disease; hunting is illegal, ship strikes and entrapments in fishing gear are rare, ice entrapments and predation are unknown. Among beach-cast carcasses recovered and necropsied, about 23% of the adults have malignant cancers, while most of the juveniles have pneumonia; other pathological conditions are diverse. No factors are known to be limiting numbers of this population. Habitat quality factors, including persistent contaminants, boat traffic and harassment, may affect the population’s rate of increase, but these effects have not been quantitatively evaluated. Comprehensive legislation exists with powers to protect the population and the environment of which it is a component, but application and enforcement of the laws is not without problems.

  9. Exploring Viral Genomics at Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Kilpatrick, K; Hiddessen, A

    2007-08-22

    This summer I had the privilege of working at Lawrence Livermore National Laboratory under the Nonproliferation, Homeland and International Security Directorate in the Chemical and Biological Countermeasures Division. I worked exclusively on the Viral Identification and Characterization Initiative (VICI) project focusing on the development of multiplexed polymerase chain reaction (PCR) assays. The goal of VICI is to combine several disciplines such as molecular biology, microfluidics, and bioinformatics in order to detect viruses and identify them in order to effectively and quickly counter infectious disease, natural or engineered. The difficulty in such a countermeasure is that little is known about viral diversity due to the ever changing nature of these organisms. In response, VICI is developing a new microfluidic bioanalytical platform to detect known and unknown viruses by analyzing every virus in a sample by isolating them into picoliter sized droplets on a microchip and individually analyzing them. The sample will be injected into a channel of oil to form droplets that will contain viral nucleic acids that will be amplified using PCR. The multiplexed PCR assay will produce a series of amplicons for a particular virus genome that provides an identifying signature. A device will then detect whether or not DNA is present in the droplet and will sort the empty droplets from the rest. From this point, the amplified DNA is released from the droplets and analyzed using capillary gel electrophoresis in order to read out the series of amplicons and thereby determine the identity of each virus. The following figure depicts the microfluidic process. For the abovementioned microfluidic process to work, a method for detecting amplification of target viral nucleic acids that does not interfere with the multiplexed biochemical reaction is required for downstream sorting and analysis. In this report, the successful development of a multiplexed PCR assay using SYBR Green I

  10. Lawrence Berkeley Laboratory Institutional Plan, FY 1993--1998

    Energy Technology Data Exchange (ETDEWEB)

    Chew, Joseph T.; Stroh, Suzanne C.; Maio, Linda R.; Olson, Karl R.; Grether, Donald F.; Clary, Mary M.; Smith, Brian M.; Stevens, David F.; Ross, Loren; Alper, Mark D.; Dairiki, Janis M.; Fong, Pauline L.; Bartholomew, James C.

    1992-10-01

    The FY 1993--1998 Institutional Plan provides an overview of the Lawrence Berkeley Laboratory mission, strategic plan, scientific initiatives, research programs, environment and safety program plans, educational and technology transfer efforts, human resources, and facilities needs. The Strategic Plan section identifies long-range conditions that can influence the Laboratory, potential research trends, and several management implications. The Initiatives section identifies potential new research programs that represent major long-term opportunities for the Laboratory and the resources required for their implementation. The Scientific and Technical Programs section summarizes current programs and potential changes in research program activity. The Environment, Safety, and Health section describes the management systems and programs underway at the Laboratory to protect the environment, the public, and the employees. The Technology Transfer and Education programs section describes current and planned programs to enhance the nation`s scientific literacy and human infrastructure and to improve economic competitiveness. The Human Resources section identifies LBL staff composition and development programs. The section on Site and Facilities discusses resources required to sustain and improve the physical plant and its equipment. The Resource Projections are estimates of required budgetary authority for the Laboratory`s ongoing research programs. The plan is an institutional management report for integration with the Department of Energy`s strategic planning activities that is developed through an annual planning process. The plan identifies technical and administrative directions in the context of the National Energy Strategy and the Department of Energy`s program planning initiatives. Preparation of the plan is coordinated by the Office for Planning and Development from information contributed by the Laboratory`s scientific and support divisions.

  11. Study of the <mi>Zc>+ channel using lattice QCD

    Energy Technology Data Exchange (ETDEWEB)

    Prelovsek, Sasa; Lang, C. B.; Leskovec, Luka; Mohler, Daniel

    2015-01-01

    Recently experimentalists have discovered several charged charmoniumlike hadrons mi>Zmi>mi>cmi>+ with unconventional quark content mi>c>¯mi>c>mi>d>¯mi>u>. We perform a search for mi>Zmi>mi>cmi>+ with mass below 4.2 GeV in the channel mi>Imi>mi>G>(mi>Jmi>mi>Pmi>mi>C>)=1+(1+-) using lattice QCD. The major challenge is presented by the two-meson states mi>Jmi>/mi>ψmi>π>, mi>ψ>2mi>Smi>mi>π>, mi>ψ>1mi>Dmi>

  12. La “nueva” Doctrina Monroe de 1895 y sus implicaciones para el Caribe español: algunas interpretaciones coetáneas españolas

    Directory of Open Access Journals (Sweden)

    Hilton, Sylvia L.

    1998-06-01

    Full Text Available Not available.

    El análisis de una selección de periódicos españoles de diversas ideologías políticas demuestra que la mayoría de los comentaristas relacionaban la política de Cleveland en la disputa anglo-venezolana en 1895 con la de McKinley en la crisis colonial española de 1895-1898, interpretando ambas intervenciones como una función de la Doctrina Monroe. Sin embargo, al contrario que el presidente Cleveland, McKinley no apeló a esta Doctrina como justificación de su política exterior iberoamericana o de su actitud ante la guerra hispano- cubana, por lo que se interpreta que las referencias a ella en el discurso periodístico peninsular de estos años obedecen a móviles más relacionados con los resortes y objetivos de la propia retórica periodística, dentro del contexto de la política española, tanto doméstica y colonial como internacional. Esto explica las contradicciones y diferencias interpretativas en torno a la Doctrina Monroe que se reflejan en la prensa española.

  13. Toxic compounds and health and reproductive effects in St. Lawrence Beluga Whales

    Energy Technology Data Exchange (ETDEWEB)

    Beland, P.; Michaud, R. (St. Lawrence National Inst. of Ecotoxicology, Montreal, Quebec (Canada)); DeGuise, S. (St. Lawrence National Inst. of Ecotoxicology, Montreal, Quebec (Canada) Faculte de Medecine Veterinaire, St-Hyacinthe, Quebec (Canada)); Girard, C.; Lagace, A. (Faculte de Medecine Veterinaire, St-Hyacinthe, Quebec (Canada)); Martineau, D. (St. Lawrence National Inst. of Ecotoxicology, Montreal, Quebec (Canada) Cornell Univ., Ithaca, NY (United States)); Muir, D.C.G. (Freshwater Inst., Winnipeg, Manitoba (Canada)); Norstorm, R.J. (National Wildlife Research Center, Hull, Quebec (Canada)); Pelletier, E. (INRS-Oceanologie, Rimouski, Quebec (Canada)); Ray, S. (Fisheries and Oceans Canada, St. John' s, Newfoundland (Canada)) (and others)

    1993-01-01

    An epidemiologic study was carried out over a period of 9 years on an isolated population of beluga whales (Delphinapterus leucas) residing in the St. Lawrence estuary (Quebec, Canada). More than 100 individual deaths were aged, and/or autopsied and analyzed for toxic compounds, and the population was surveyed for size and structure. Arctic belugas and other species of whales and seals from the St. Lawrence were used for comparison. Population dynamics: Population size appeared to be stable and modeling showed this stable pattern to result from low calf production and/or low survival to adulthood. Toxicology: St. Lawrence belugas had higher or much higher levels of mercury, lead, PCBs, DDT, Mirex, benzo[a]pyrene metabolites, equivalent levels of dioxins, furans, and PAH metabolites, and much lower levels of cadmium than Arctic belugas. In other St. Lawrence cetaceans, levels of PCBs and DDT were inversely related to body size, as resulting from differences in metabolic rate, diet, and trophic position, compounded by length of residence in the St. Lawrence basin. St. Lawrence belugas had much higher levels than predicted from body size alone; levels increased with age in both sexes, although unloading by females through the placenta and/or lactation was evidenced by overall lower levels in females and very high burdens in some calves. 45 refs., 4 figs., 5 tabs.

  14. Inspection Report "Personal Property Management at Lawrence Livermore National Laboratory"

    Energy Technology Data Exchange (ETDEWEB)

    None

    2009-05-01

    The Department of Energy's (DOE's) Lawrence Livermore National Laboratory (Livermore) is a premier research and development institution for science and technology supporting the core mission of national security. According to Livermore, as of November 2008 the Laboratory managed 64,933 items of Government personal property valued at about $1 billion. At the beginning of Fiscal Year 2008, Livermore reported 249 DOE property items valued at about $1.3 million that were missing, unaccounted for, or stolen during Fiscal Year 2007. Livermore centrally tracks property utilizing the Sunflower Assets system (Sunflower), which reflects the cradle to grave history of each property item. Changes in the custodianship and/or location of a property item must be timely reported by the custodian to the respective property center representative for updating in Sunflower. In Fiscal Year 2008, over 2,000 individuals were terminated as a result of workforce reduction at Livermore, of which about 750 received a final notification of termination on the same day that they were required to depart the facility. All of these terminations potentially necessitated updates to the property database, but the involuntary terminations had the potential to pose particular challenges because of the immediacy of individuals departures. The objective of our inspection was to evaluate the adequacy of Livermore's internal controls over Government property. Based upon the results of our preliminary field work, we particularly focused on personal property assigned to terminated individuals and stolen laptop computers. We concluded that Livermore's internal controls over property could be improved, which could help to reduce the number of missing, unaccounted for, or stolen property items. Specifically, we found that: (1) The location and/or custodian of approximately 18 percent of the property items in our sample, which was drawn from the property assigned to individuals terminated on

  15. Lawrence Berkeley Laboratory Institutional Plan FY 1987-1992

    Energy Technology Data Exchange (ETDEWEB)

    Various

    1986-12-01

    The Lawrence Berkeley Laboratory, operated by the University of California for the Department of Energy, provides national scientific leadership and supports technological innovation through its mission to: (1) Perform leading multidisciplinary research in general sciences and energy sciences; (2) Develop and operate unique national experimental facilities for use by qualified investigators; (3) Educate and train future generations of scientists and engineers; and (4) Foster productive relationships between LBL research programs and industry. The following areas of research excellence implement this mission and provide current focus for achieving DOE goals. GENERAL SCIENCES--(1) Accelerator and Fusion Research--accelerator design and operation, advanced accelerator technology development, accelerator and ion source research for heavy-ion fusion and magnetic fusion, and x-ray optics; (2) Nuclear Science--relativistic heavy-ion physics, medium- and low-energy nuclear physics, nuclear theory, nuclear astrophysics, nuclear chemistry, transuranium elements studies, nuclear data evaluation, and detector development; (3) Physics--experimental and theoretical particle physics, detector development, astrophysics, and applied mathematics. ENERGY SCIENCES--(1) Applied Science--building energy efficiency, solar for building systems, fossil energy conversion, energy storage, and atmospheric effects of combustion; (2) Biology and Medicine--molecular and cellular biology, diagnostic imaging, radiation biophysics, therapy and radiosurgery, mutagenesis and carcinogenesis, lipoproteins, cardiovascular disease, and hemopoiesis research; (3) Center for Advanced Materials--catalysts, electronic materials, ceramic and metal interfaces, polymer research, instrumentation, and metallic alloys; (4) Chemical Biodynamics--molecular biology of nucleic acids and proteins, genetics of photosynthesis, and photochemistry; (5) Earth Sciences--continental lithosphere properties, structures and

  16. Generation of miRNA sponge constructs

    NARCIS (Netherlands)

    Kluiver, Joost; Slezak-Prochazka, Izabella; Smigielska-Czepiel, Katarzyna; Halsema, Nancy; Kroesen, Bart-Jan; van den Berg, Anke

    2012-01-01

    MicroRNA (miRNA) sponges are RNA molecules with repeated miRNA antisense sequences that can sequester miRNAs from their endogenous targets and thus serve as a decoy. Stably expressed miRNA sponges are especially valuable for long-term loss-of-function studies and can be used in vitro and in vivo. We

  17. Interactions of miR-323/miR-326/miR-329 and miR-130a/miR-155/miR-210 as prognostic indicators for clinical outcome of glioblastoma patients

    Directory of Open Access Journals (Sweden)

    Qiu Shuwei

    2013-01-01

    Full Text Available Abstract Background Glioblastoma multiforme (GBM is the most common and aggressive brain tumor with poor clinical outcome. Identification and development of new markers could be beneficial for the diagnosis and prognosis of GBM patients. Deregulation of microRNAs (miRNAs or miRs is involved in GBM. Therefore, we attempted to identify and develop specific miRNAs as prognostic and predictive markers for GBM patient survival. Methods Expression profiles of miRNAs and genes and the corresponding clinical information of 480 GBM samples from The Cancer Genome Atlas (TCGA dataset were downloaded and interested miRNAs were identified. Patients’ overall survival (OS and progression-free survival (PFS associated with interested miRNAs and miRNA-interactions were performed by Kaplan-Meier survival analysis. The impacts of miRNA expressions and miRNA-interactions on survival were evaluated by Cox proportional hazard regression model. Biological processes and network of putative and validated targets of miRNAs were analyzed by bioinformatics. Results In this study, 6 interested miRNAs were identified. Survival analysis showed that high levels of miR-326/miR-130a and low levels of miR-323/miR-329/miR-155/miR-210 were significantly associated with long OS of GBM patients, and also showed that high miR-326/miR-130a and low miR-155/miR-210 were related with extended PFS. Moreover, miRNA-323 and miRNA-329 were found to be increased in patients with no-recurrence or long time to progression (TTP. More notably, our analysis revealed miRNA-interactions were more specific and accurate to discriminate and predict OS and PFS. This interaction stratified OS and PFS related with different miRNA levels more detailed, and could obtain longer span of mean survival in comparison to that of one single miRNA. Moreover, miR-326, miR-130a, miR-155, miR-210 and 4 miRNA-interactions were confirmed for the first time as independent predictors for survival by Cox regression model

  18. Down-regulated miR-9 and miR-433 in human gastric carcinoma

    Directory of Open Access Journals (Sweden)

    Nie Na

    2009-06-01

    Full Text Available Abstract Background MircoRNAs(miRNAs are short, endogenously non-coding RNAs. The abnormal expression of miRNAs may be valuable for the diagnosis and treatment of tumors. Methods To screening the special miRNAs in gastric carcinoma, expression level of miRNAs in gastric carcinoma and normal gaster samples were detected by miRNA gene chip. Then, the expressions of miR-9 and miR-433 in gastric carcinoma tissue and SGC7901 cell line were validated by qRT-PCR. GRB2 and RAB34, targets of miR-433 and miR-9 respectively, were detected by Western blot. Results We found 19 miRNAs and 7 miRNAs were down-regulated and up-regulated respectively. Compared with normal gaster samples, our data showed that miR-9 and miR-433 were down-regulated in gastric carcinoma. Meanwhile, we also found that miR-433 and miR-9 regulated the expression levels of GRB2 and RAB34 respectively. Conclusion Our data show miR-9 and miR-433 was down-regulated in gastric carcinoma. The targets of miR-433 and miR-9 were tumor-associated proteins GRB2 and RAB34 respectively. This result provided the related information of miRNAs in gastric carcinoma.

  19. DHS-STEM Internship at Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Feldman, B

    2008-08-18

    This summer I had the fortunate opportunity through the DHS-STEM program to attend Lawrence Livermore National Laboratories (LLNL) to work with Tom Slezak on the bioinformatics team. The bioinformatics team, among other things, helps to develop TaqMan and microarray probes for the identification of pathogens. My main project at the laboratory was to test such probe identification capabilities against metagenomic (unsequenced) data from around the world. Using various sequence analysis tools (Vmatch and Blastall) and several we developed ourselves, about 120 metagenomic sequencing projects were compared against a collection of all completely sequenced genomes and Lawrence Livermore National Laboratory's (LLNL) current probe database. For the probes, the Blastall algorithms compared each individual metagenomic project using various parameters allowing for the natural ambiguities of in vitro hybridization (mismatches, deletions, insertions, hairpinning, etc.). A low level cutoff was used to eliminate poor sequence matches, and to leave a large variety of higher quality matches for future research into the hybridization of sequences with mutations and variations. Any hits with at least 80% base pair conservation over 80% of the length of the match. Because of the size of our whole genome database, we utilized the exact match algorithm of Vmatch to quickly search and compare genomes for exact matches with varying lower level limits on sequence length. I also provided preliminary feasibility analyses to support a potential industry-funded project to develop a multiplex assay on several genera and species. Each genus and species was evaluated based on the amount of sequenced genomes, amount of near neighbor sequenced genomes, presence of identifying genes--metabolistic or antibiotic resistant genes--and the availability of research on the identification of the specific genera or species. Utilizing the bioinformatic team's software, I was able to develop and

  20. Review of miRNA Technology

    Institute of Scientific and Technical Information of China (English)

    Zhang Tangyao

    2015-01-01

    miRNAs are non-coding RNA molecules exist in eukaryotic with 22 nucleotides. The abnormal expression of miRNAs are also lead to somedisease. The monitoring of cancer related miRNAs, oncomiRs, will help diagnose caners. The main methods to analyzing the profile of miRNA expression fordiagnosing cancer are microarray test and real-time PCR. The the studies on miRomics will bring revolutionary breakthrough to medicine and carcinobiology.

  1. miRNAs Related to Skeletal Diseases.

    Science.gov (United States)

    Seeliger, Claudine; Balmayor, Elizabeth R; van Griensven, Martijn

    2016-09-01

    miRNAs as non-coding, short, double-stranded RNA segments are important for cellular biological functions, such as proliferation, differentiation, and apoptosis. miRNAs mainly contribute to the inhibition of important protein translations through their cleavage or direct repression of target messenger RNAs expressions. In the last decade, miRNAs got in the focus of interest with new publications on miRNAs in the context of different diseases. For many types of cancer or myocardial damage, typical signatures of local or systemically circulating miRNAs have already been described. However, little is known about miRNA expressions and their molecular effect in skeletal diseases. An overview of published studies reporting miRNAs detection linked with skeletal diseases was conducted. All regulated miRNAs were summarized and their molecular interactions were illustrated. This review summarizes the involvement and interaction of miRNAs in different skeletal diseases. Thereby, 59 miRNAs were described to be deregulated in tissue, cells, or in the circulation of osteoarthritis (OA), 23 miRNAs deregulated in osteoporosis, and 107 miRNAs deregulated in osteosarcoma (OS). The molecular influences of miRNAs regarding OA, osteoporosis, and OS were illustrated. Specific miRNA signatures for skeletal diseases are described in the literature. Some overlapped, but also unique ones for each disease exist. These miRNAs may present useful targets for the development of new therapeutic approaches and are candidates for diagnostic evaluations.

  2. Lawrence Livermore National Laboratory Surface Water Protection: A Watershed Approach

    Energy Technology Data Exchange (ETDEWEB)

    Coty, J

    2009-03-16

    This surface water protection plan (plan) provides an overview of the management efforts implemented at Lawrence Livermore National Laboratory (LLNL) that support a watershed approach to protect surface water. This plan fulfills a requirement in the Department of Energy (DOE) Order 450.1A to demonstrate a watershed approach for surface water protection that protects the environment and public health. This plan describes the use of a watershed approach within which the Laboratory's current surface water management and protections efforts have been structured and coordinated. With more than 800 million acres of land in the U.S. under federal management and stewardship, a unified approach across agencies provides enhanced resource protection and cost-effectiveness. The DOE adopted, along with other federal agencies, the Unified Federal Policy for a Watershed Approach to Federal Land and Resource Management (UFP) with a goal to protect water quality and aquatic ecosystems on federal lands. This policy intends to prevent and/or reduce water pollution from federal activities while fostering a cost-effective watershed approach to federal land and resource management. The UFP also intends to enhance the implementation of existing laws (e.g., the Clean Water Act [CWA] and National Environmental Policy Act [NEPA]) and regulations. In addition, this provides an opportunity for the federal government to serve as a model for water quality stewardship using a watershed approach for federal land and resource activities that potentially impact surface water and its uses. As a federal land manager, the Laboratory is responsible for a small but important part of those 800 million acres of land. Diverse land uses are required to support the Laboratory's mission and provide an appropriate work environment for its staff. The Laboratory comprises two sites: its main site in Livermore, California, and the Experimental Test Site (Site 300), near Tracy, California. The main site

  3. Genetic variation of the St. Lawrence beluga whale population assessed by DNA fingerprinting.

    Science.gov (United States)

    Patenaude, N J; Quinn, J S; Beland, P; Kingsley, M; White, B N

    1994-08-01

    Recent surveys suggest that the endangered St. Lawrence beluga (Delphinapterus leucas) population is not recovering significantly despite 20 years of protection. Dead individuals that have been autopsied show high levels of tumours and infections. This situation could be a result of pollution, loss of genetic variation, inbreeding depression or a combination of these factors. Analyses of DNA fingerprints from St. Lawrence belugas with three minisatellite probes (Jeffreys 33.6, 33.15 and M13) indicate a reduced level of genetic variation compared to Beaufort Sea animals. The average band-sharing between individuals of the St. Lawrence beluga population for the three probes (0.534, 0.573 and 0.478, respectively) was significantly higher than that of the Beaufort Sea beluga population (0.343, 0.424, 0.314, respectively). Higher levels of mean allele frequency in the St. Lawrence belugas (0.33 vs. 0.21) suggest that this population is composed of individuals which are related. Inbreeding depression could therefore be a factor in the lack of recovery of the St. Lawrence beluga population.

  4. Circulating microRNAs, miR-939, miR-595, miR-519d and miR-494, Identify Cirrhotic Patients with HCC.

    Directory of Open Access Journals (Sweden)

    Francesca Fornari

    Full Text Available The performance of circulating biomarkers for the diagnosis of hepatocellular carcinoma (HCC is sub-optimal. In this study we tested circulating microRNAs as biomarkers for HCC in cirrhotic patients by performing a two stage study: a discovery phase conducted by microarray and a validation phase performed by qRT-PCR in an independent series of 118 patients. Beside miRNAs emerged from the discovery phase, miR-21, miR-221, miR-519d were also tested in the validation setting on the basis of literary and tissue findings. Deregulated microRNAs were assayed in HCC-derived cells in the intracellular compartment, cell culture supernatant and exosomal fraction. Serum and tissue microRNA levels were compared in 14 patients surgically treated for HCC. From the discovery study, it emerged that seven circulating microRNAs were differentially expressed in cirrhotic patients with and without HCC. In the validation set, miR-939, miR-595 and miR-519d were shown to differentiate cirrhotic patients with and without HCC. MiR-939 and miR-595 are independent factors for HCC. ROC curves of miR-939, miR-595 and miR-519d displayed that AUC was higher than AFP. An exosomal secretion of miR-519d, miR-21, miR-221 and miR-1228 and a correlation between circulating and tissue levels of miR-519d, miR-494 and miR-21 were found in HCC patients. Therefore, we show that circulating microRNAs deserve attention as non-invasive biomarkers in the diagnostic setting of HCC and that exosomal secretion contributes to discharging a subset of microRNAs into the extracellular compartment.

  5. Circulating microRNAs, miR-939, miR-595, miR-519d and miR-494, Identify Cirrhotic Patients with HCC

    Science.gov (United States)

    Fornari, Francesca; Ferracin, Manuela; Trerè, Davide; Milazzo, Maddalena; Marinelli, Sara; Galassi, Marzia; Venerandi, Laura; Pollutri, Daniela; Patrizi, Clarissa; Borghi, Alberto; Foschi, Francesco G.; Stefanini, Giuseppe F.; Negrini, Massimo; Bolondi, Luigi; Gramantieri, Laura

    2015-01-01

    The performance of circulating biomarkers for the diagnosis of hepatocellular carcinoma (HCC) is sub-optimal. In this study we tested circulating microRNAs as biomarkers for HCC in cirrhotic patients by performing a two stage study: a discovery phase conducted by microarray and a validation phase performed by qRT-PCR in an independent series of 118 patients. Beside miRNAs emerged from the discovery phase, miR-21, miR-221, miR-519d were also tested in the validation setting on the basis of literary and tissue findings. Deregulated microRNAs were assayed in HCC-derived cells in the intracellular compartment, cell culture supernatant and exosomal fraction. Serum and tissue microRNA levels were compared in 14 patients surgically treated for HCC. From the discovery study, it emerged that seven circulating microRNAs were differentially expressed in cirrhotic patients with and without HCC. In the validation set, miR-939, miR-595 and miR-519d were shown to differentiate cirrhotic patients with and without HCC. MiR-939 and miR-595 are independent factors for HCC. ROC curves of miR-939, miR-595 and miR-519d displayed that AUC was higher than AFP. An exosomal secretion of miR-519d, miR-21, miR-221 and miR-1228 and a correlation between circulating and tissue levels of miR-519d, miR-494 and miR-21 were found in HCC patients. Therefore, we show that circulating microRNAs deserve attention as non-invasive biomarkers in the diagnostic setting of HCC and that exosomal secretion contributes to discharging a subset of microRNAs into the extracellular compartment. PMID:26509672

  6. miRConnect: identifying effector genes of miRNAs and miRNA families in cancer cells.

    Directory of Open Access Journals (Sweden)

    Youjia Hua

    Full Text Available micro(miRNAs are small non-coding RNAs that negatively regulate expression of most mRNAs. They are powerful regulators of various differentiation stages, and the expression of genes that either negatively or positively correlate with expressed miRNAs is expected to hold information on the biological state of the cell and, hence, of the function of the expressed miRNAs. We have compared the large amount of available gene array data on the steady state system of the NCI60 cell lines to two different data sets containing information on the expression of 583 individual miRNAs. In addition, we have generated custom data sets containing expression information of 54 miRNA families sharing the same seed match. We have developed a novel strategy for correlating miRNAs with individual genes based on a summed Pearson Correlation Coefficient (sPCC that mimics an in silico titration experiment. By focusing on the genes that correlate with the expression of miRNAs without necessarily being direct targets of miRNAs, we have clustered miRNAs into different functional groups. This has resulted in the identification of three novel miRNAs that are linked to the epithelial-to-mesenchymal transition (EMT in addition to the known EMT regulators of the miR-200 miRNA family. In addition, an analysis of gene signatures associated with EMT, c-MYC activity, and ribosomal protein gene expression allowed us to assign different activities to each of the functional clusters of miRNAs. All correlation data are available via a web interface that allows investigators to identify genes whose expression correlates with the expression of single miRNAs or entire miRNA families. miRConnect.org will aid in identifying pathways regulated by miRNAs without requiring specific knowledge of miRNA targets.

  7. miRConnect: identifying effector genes of miRNAs and miRNA families in cancer cells.

    Science.gov (United States)

    Hua, Youjia; Duan, Shiwei; Murmann, Andrea E; Larsen, Niels; Kjems, Jørgen; Lund, Anders H; Peter, Marcus E

    2011-01-01

    micro(mi)RNAs are small non-coding RNAs that negatively regulate expression of most mRNAs. They are powerful regulators of various differentiation stages, and the expression of genes that either negatively or positively correlate with expressed miRNAs is expected to hold information on the biological state of the cell and, hence, of the function of the expressed miRNAs. We have compared the large amount of available gene array data on the steady state system of the NCI60 cell lines to two different data sets containing information on the expression of 583 individual miRNAs. In addition, we have generated custom data sets containing expression information of 54 miRNA families sharing the same seed match. We have developed a novel strategy for correlating miRNAs with individual genes based on a summed Pearson Correlation Coefficient (sPCC) that mimics an in silico titration experiment. By focusing on the genes that correlate with the expression of miRNAs without necessarily being direct targets of miRNAs, we have clustered miRNAs into different functional groups. This has resulted in the identification of three novel miRNAs that are linked to the epithelial-to-mesenchymal transition (EMT) in addition to the known EMT regulators of the miR-200 miRNA family. In addition, an analysis of gene signatures associated with EMT, c-MYC activity, and ribosomal protein gene expression allowed us to assign different activities to each of the functional clusters of miRNAs. All correlation data are available via a web interface that allows investigators to identify genes whose expression correlates with the expression of single miRNAs or entire miRNA families. miRConnect.org will aid in identifying pathways regulated by miRNAs without requiring specific knowledge of miRNA targets.

  8. Fort Monroe Historic Viewsheds

    Science.gov (United States)

    2010-08-01

    of North America.2 These groups also appear to have had a strong in- terest in high quality , lithic material found farther west in the state. During...very large multi-band camps through smaller band camps to very small micro-band foraging sites. In matters of subsistence there may be differences...show evidence of maize agriculture. It was the groups of the Late Woodland who would encounter the European explorers in the late sixteenth and

  9. Merilin Monro : Posledneje intervju

    Index Scriptorium Estoniae

    2002-01-01

    Studio Universal esitles dokumentaalfilmi "Marilyn - viimane intervjuu", mille aluseks on ajakirja "Life" ajakirjaniku 8-tunnine intervjuu näitlejatariga, tehtud paar päeva enne viimase siiani salapärast surma

  10. Merilin Monro : Posledneje intervju

    Index Scriptorium Estoniae

    2002-01-01

    Studio Universal esitles dokumentaalfilmi "Marilyn - viimane intervjuu", mille aluseks on ajakirja "Life" ajakirjaniku 8-tunnine intervjuu näitlejatariga, tehtud paar päeva enne viimase siiani salapärast surma

  11. HYDROLOGY, MONROE COUNTY, MISSISSIPPI

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Hydrology data include spatial datasets and data tables necessary for documenting the hydrologic procedures for estimating flood discharges for a flood insurance...

  12. Reference miRNAs for miRNAome analysis of urothelial carcinomas.

    Directory of Open Access Journals (Sweden)

    Nadine Ratert

    Full Text Available BACKGROUND/OBJECTIVE: Reverse transcription quantitative real-time PCR (RT-qPCR is widely used in microRNA (miRNA expression studies on cancer. To compensate for the analytical variability produced by the multiple steps of the method, relative quantification of the measured miRNAs is required, which is based on normalization to endogenous reference genes. No study has been performed so far on reference miRNAs for normalization of miRNA expression in urothelial carcinoma. The aim of this study was to identify suitable reference miRNAs for miRNA expression studies by RT-qPCR in urothelial carcinoma. METHODS: Candidate reference miRNAs were selected from 24 urothelial carcinoma and normal bladder tissue samples by miRNA microarrays. The usefulness of these candidate reference miRNAs together with the commonly for normalization purposes used small nuclear RNAs RNU6B, RNU48, and Z30 were thereafter validated by RT-qPCR in 58 tissue samples and analyzed by the algorithms geNorm, NormFinder, and BestKeeper. PRINCIPAL FINDINGS: Based on the miRNA microarray data, a total of 16 miRNAs were identified as putative reference genes. After validation by RT-qPCR, miR-101, miR-125a-5p, miR-148b, miR-151-5p, miR-181a, miR-181b, miR-29c, miR-324-3p, miR-424, miR-874, RNU6B, RNU48, and Z30 were used for geNorm, NormFinder, and BestKeeper analyses that gave different combinations of recommended reference genes for normalization. CONCLUSIONS: The present study provided the first systematic analysis for identifying suitable reference miRNAs for miRNA expression studies of urothelial carcinoma by RT-qPCR. Different combinations of reference genes resulted in reliable expression data for both strongly and less strongly altered miRNAs. Notably, RNU6B, which is the most frequently used reference gene for miRNA studies, gave inaccurate normalization. The combination of four (miR-101, miR-125a-5p, miR-148b, and miR-151-5p or three (miR-148b, miR-181b, and miR-874

  13. Supplement Analysis for the Transmission System Vegetation Management Program FEIS (DOE/EIS-0285/SA-106)SnoKing Tap to Monroe-Samamish Line

    Energy Technology Data Exchange (ETDEWEB)

    Martin, Mark A. [Bonneville Power Administration, Portland, OR (United States)

    2002-09-30

    Vegetation Management along the SnoKing Tap to the Monroe-Samamish transmission line from structure 8/1 through structure 20/6. BPA proposes to remove unwanted vegetation along the right-of-way, access roads and around tower structures along the subject transmission line corridor. The right-of-way will be treated using selective and non-selective methods that include hand cutting, mowing and herbicide treatments. Approximately 12 miles of access roads will be cleared using selective and non-selective methods that include hand cutting, mowing and herbicide treatments. Approximately 65 tower sites will be treated using selective and non-selective methods that include hand cutting, mowing and herbicide treatments. Vegetation management is required for unimpeded operation and maintenance of the subject transmission line. See Section 1of the attached checklist for a complete description of the proposal.

  14. La “nueva” Doctrina Monroe de 1895 y sus implicaciones para el Caribe español: algunas interpretaciones coetáneas españolas

    OpenAIRE

    1998-01-01

    Not available.

    El análisis de una selección de periódicos españoles de diversas ideologías políticas demuestra que la mayoría de los comentaristas relacionaban la política de Cleveland en la disputa anglo-venezolana en 1895 con la de McKinley en la crisis colonial española de 1895-1898, interpretando ambas intervenciones como una función de la Doctrina Monroe. Sin embargo, al contrario que el presidente Cleveland, McKinley no apeló a esta Doctrina como justificación de su p...

  15. The Tumor Cytosol miRNAs, Fluid miRNAs and Exosome miRNAs in Lung Cancer

    Directory of Open Access Journals (Sweden)

    Xin eQin

    2015-01-01

    Full Text Available The focus of this review is to provide an update on the progress of microRNAs (miRNAs as potential biomarkers for lung cancer. miRNAs are single-stranded, small noncoding RNAs that regulate gene expression and show tissue-specific signatures. Accumulating evidence indicates that miRNA expression patterns represent the in vivo status in physiology and disease. Moreover, miRNAs are stable in serum and other clinically convenient and available tissue sources, so they are being developed as biomarkers for cancer and other diseases. Cancer is currently the primary driver of the field, but miRNA biomarkers are being developed for many other diseases such as cardiovascular and central nervous system diseases. Here we examine the framework and scope of the miRNA landscape as it specifically relates to the translation of miRNA expression patterns/signatures into biomarkers for developing diagnostics for lung cancer. We focus on examining tumor cytosol miRNAs, fluid miRNAs, and exosome miRNAs in lung cancer, the connections among these miRNAs, and the potential of miRNA biomarkers for the development of diagnostics. In lung cancer, miRNAs have been studied in both cell populations and in the circulation. However, a major challenge is to develop biomarkers to monitor cancer development and to identify circulating miRNAs that are linked to cancer stage. Importantly, the fact that miRNAs can be successfully harvested from biological fluids allows for the development of biofluid biopsies, in which miRNAs as circulating biomarkers can be captured and analyzed ex vivo. Our hope is that these minimally invasive entities provide a window to the in vivo milieu of the patients without the need for costly, complex invasive procedures, rapidly moving miRNAs from research to the clinic.

  16. Mi ángel protector

    OpenAIRE

    Alvarado Calvache, Marian Seinef; Pontificia Universidad Javeriana, Cali

    2013-01-01

    Un día, una hora, un minuto o quizás un segundo. Cuando se trata de tomar decisiones, si no te das prisa puedes arrepentirte el resto de tu vida. ¿Una prueba de esto? Bueno, aquí está mi historia. Tengo vagos recuerdos de lo que pasó ese día. Pero el sentimiento que me invadió cuando desperté sola, en un hospital y con mi ropa ensangrentada, es difícil de olvidar aun cuando solo tenía ocho años. 22 de Abril de 2002, 7:00 p.m. – Chao mami, le dije, mientras me subía al carro junto con mi padre...

  17. Interpretation of D. H. Lawrence,s Human Ecological View%D.H劳伦斯的人文生态观解读

    Institute of Scientific and Technical Information of China (English)

    马巧正

    2012-01-01

    D. H. Lawrence was one of the rather controversial writers in Britain and even in the world. In his works, Lawrence extolled the pure natural beauty and criticized the "degeneration" of the environment and human civilization brought about by the industrial revolution. This paper, from four aspects, interprets D. H. Lawrence human ecological view: Man and Nature should keep a harmonious and balanced relationship, k is hoped that peo- ple ecological awareness can be aroused in order to realize the harmonious and perfect ecological balance between Man and Nature.%劳伦斯是20世纪英国乃至世界文坛颇具争议的作家之一。劳伦斯在他的作品中,以独特的创作视角,歌颂纯粹的自然美,批判现代工业文明对环境和人类文明的"污浊"。从三个方面解读劳伦斯的人文生态观:人与自然应该保持和谐共处、息息相关的平衡关系。希望能够唤起人们的生态意识,以期实现人类与自然界和谐完美的生态平衡。

  18. Cenas de um casamento: o corpo erótico em D. H. Lawrence

    Directory of Open Access Journals (Sweden)

    Maria Conceição Monteiro

    2010-01-01

    Full Text Available In Lady Chatterley’s Lover, D. H. Lawrence had in mind to eliminate both the extremes of an apparent Victorian modesty and the decadence and the modern mechanization of his time, showing the necessity of a revolutionary change with reference to sexual attitudes. For Lawrence, sexuality does not simply belong to the physical body, but also to a complex of fantasies and symbolizations that determine identity.My reading of the novel analyses the body as agent and object of desire, desire that if on the one hand is sexual, on the other is a desire for knowledge.

  19. Identification of miRNAs and miRNA-mediated regulatory pathways in Carica papaya.

    Science.gov (United States)

    Liang, Gang; Li, Yang; He, Hua; Wang, Fang; Yu, Diqiu

    2013-10-01

    Plant microRNAs (miRNAs) post-transcriptionally regulate target gene expression to modulate growth and development and biotic and abiotic stress responses. By analyzing small RNA deep sequencing data in combination with the genome sequence, we identified 75 conserved miRNAs and 11 novel miRNAs. Their target genes were also predicted. For most conserved miRNAs, the miRNA-target pairs were conserved across plant species. In addition to these conserved miRNA-target pairs, we also identified some papaya-specific miRNA-target regulatory pathways. Both miR168 and miR530 target the Argonaute 1 gene, indicating a second autoregulatory mechanism for miRNA regulation. A non-conserved miRNA was mapped within an intron of Dicer-like 1 (DCL1), suggesting a conserved homeostatic autoregulatory mechanism for DCL1 expression. A 21-nt miRNA triggers secondary siRNA production from its target genes, nucleotide-binding site leucine-rich repeat protein genes. Certain phased-miRNAs were processed from their conserved miRNA precursors, indicating a putative miRNA evolution mechanism. In addition, we identified a Carica papaya-specific miRNA that targets an ethylene receptor gene, implying its function in the ethylene signaling pathway. This work will also advance our understanding of miRNA functions and evolution in plants.

  20. New miRNAs cloned from neuroblastoma

    Directory of Open Access Journals (Sweden)

    Glatting Karl-Heinz

    2008-01-01

    Full Text Available Abstract Background MicroRNAs (miRNAs are a novel class of gene expression regulators implicated in cancer biology. Neuroblastoma (NB is an embryonal tumour consisting of neural crest-derived undifferentiated cells and is characterised by variable clinical courses ranging from spontaneous regression to therapy-resistant progression. Recent advances identified a subset of miRNAs with putative function in NB biology. However, the full repertoire of miRNAs expressed in NBs is not available. Results We describe miRNA profiles of 13 NB specimens and 2 NB cell lines as determined by miRNA cloning. A total of 3153 sequences were sequenced and analysed by a miRNA prediction tool (miRpredict. Our library covered 27% miRNAs known to date. 39 reads corresponding to 25 individual sequences were classified as novel miRNAs, including miRNA* species of 10 known miRNAs. Expression of 5 new miRNA* forms and 8 individual sequences was supported by Northern blotting. Most of the novel miRNA genes are not related to each other and do not share homology with the annotated sequences in the public miRNA database, but they are conserved within mammals or have close homologues in primates genomes. Conclusion We provide evidence for 29 new miRNA and miRNA-like sequences (24 novel sequences and 5 miRNAs discovered initially in other species. Some of these newly identified sequences reside within frequently altered chromosomal regions in NB tumours and may play a role in NB biology.

  1. 75 FR 4822 - Decision To Evaluate a Petition To Designate a Class of Employees for the Lawrence Livermore...

    Science.gov (United States)

    2010-01-29

    ... warranted by the evaluation, is as follows: Facility: Lawrence Livermore National Laboratory. Location... HUMAN SERVICES Decision To Evaluate a Petition To Designate a Class of Employees for the Lawrence...: Notice. SUMMARY: HHS gives notice as required by 42 CFR 83.12(e) of a decision to evaluate a petition to...

  2. Berkeley Lab's Saul Perlmutter wins E.O. Lawrence Award scientist's work on supernovae reveals accelerating universe

    CERN Multimedia

    2002-01-01

    "Saul Perlmutter, a member of Lawrence Berkeley National Laboratory's Physics Division and leader of the international Supernova Cosmology Project based there, has won the Department of Energy's 2002 E.O. Lawrence Award in the physics category" (1/2 page).

  3. Mr. Lawrence tuleb Rabarockiks kokku! Peer Günt uues kuues. Pervert ja ämma unistus

    Index Scriptorium Estoniae

    2007-01-01

    Rockansamblist Mr. Lawrence, albumitest "Mr. Lawrence", "Swing", "Sitandspin". Soome hardrock'i ansamblist Peer Günt, albumist "Backseat". Etno-folkrockansamblist Dagö (kontsert 15. juunil Rabarockil), albumitest "Toiduklubi", "Hiired tuules", "Joonistatud mees". Info festivalist: www.rabarock.delfi.ee / www.rabarock.ee

  4. Mr. Lawrence tuleb Rabarockiks kokku! Peer Günt uues kuues. Pervert ja ämma unistus

    Index Scriptorium Estoniae

    2007-01-01

    Rockansamblist Mr. Lawrence, albumitest "Mr. Lawrence", "Swing", "Sitandspin". Soome hardrock'i ansamblist Peer Günt, albumist "Backseat". Etno-folkrockansamblist Dagö (kontsert 15. juunil Rabarockil), albumitest "Toiduklubi", "Hiired tuules", "Joonistatud mees". Info festivalist: www.rabarock.delfi.ee / www.rabarock.ee

  5. 46 CFR 401.405 - Basic rates and charges on the St. Lawrence River and Lake Ontario.

    Science.gov (United States)

    2010-10-01

    ... Lake Ontario. 401.405 Section 401.405 Shipping COAST GUARD (GREAT LAKES PILOTAGE), DEPARTMENT OF... § 401.405 Basic rates and charges on the St. Lawrence River and Lake Ontario. Except as provided in.... registered pilots in the St. Lawrence River and Lake Ontario. (a) Area 1 (Designated Waters): Service...

  6. Final Report Bald and Golden Eagle Territory Surveys for the Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Fratanduono, M. L. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2014-11-25

    Garcia and Associates (GANDA) was contracted by the Lawrence Livermore National Laboratory (LLNL) to conduct surveys for bald eagles (Haliaeetus leucocephalus) and golden eagles (Aquila chrysaetos) at Site 300 and in the surrounding area out to 10-miles. The survey effort was intended to document the boundaries of eagle territories by careful observation of eagle behavior from selected viewing locations throughout the study area.

  7. Moral Maturity and Autonomy: Appreciating the Significance of Lawrence Kolhberg's Just Community

    Science.gov (United States)

    McDonough, Graham P.

    2005-01-01

    Lawrence Kohlberg's Just Community program of moral education has conceptual significance to his theoretical work in the field of moral development. This argument contends that a perspective recognizing the Just Community as conceptually significant provides a more comprehensive picture of Kohlberg's work than do critical perspectives that limit…

  8. The Legacy of Lawrence Kohlberg: Implications for Counseling and Human Development.

    Science.gov (United States)

    Hayes, Richard L.

    1994-01-01

    Notes that, despite great contributions by Lawrence Kohlberg to understanding of moral development, counselors are only beginning to appreciate fully implications of his developmental psychology for practice of counseling and human development. Draws on collective body of Kohlberg's work to show how seven assumptions have direct relevance for…

  9. Unequivocal Acceptance--Lessons from the Stephen Lawrence Inquiry for Education.

    Science.gov (United States)

    Richardson, Robin

    1999-01-01

    Explores the question of "institutional racism" in England in the context of the Stephen Lawrence murder investigation and considers the implications of the racist charges leveled at the police after this investigation for the educational system. Calls for attention to institutional racism in the British schools. (SLD)

  10. Remedial investigation and feasibility study for the Lawrence Livermore National Laboratory Site 300 Pit 7 Complex

    Energy Technology Data Exchange (ETDEWEB)

    Taffet, M.J. (Lawrence Livermore National Lab., CA (USA)); Oberdorfer, J.A. (San Jose State Univ., CA (USA)); McIlvride, W.A. (Weiss Associates, Oakland, CA (USA))

    1989-10-01

    This report summarizes the results and conclusions of the investigation of tritium and other compounds in ground water in the vicinity of landfills at the Lawrence Livermore National Laboratory (LLNL) Site 300 Pit 7 Complex. 91 refs., 110 figs., 43 tabs.

  11. 76 FR 2413 - Notice of Realty Action: Direct Sale of Public Land in Lawrence County, SD

    Science.gov (United States)

    2011-01-13

    ... Bureau of Land Management Notice of Realty Action: Direct Sale of Public Land in Lawrence County, SD... public land is being proposed for direct sale to Keith Sauls in accordance with Sections 203 and 209 of... proposing a direct sale to the homeowner, in accordance with 43 CFR ] 2711.3-3, to resolve...

  12. Lawrence Augustus Oxley: The Beginnings of Social Work among Blacks in North Carolina Counties.

    Science.gov (United States)

    Bell, John L.

    1991-01-01

    Details Lawrence Oxley's work as director of the Division of Work among Blacks from 1925 to 1934. Discusses racial attitudes and legal injustices toward Blacks. Oxley addressed social problems of Blacks such as unfair capital punishment, juvenile delinquency, and lack of social services. (KS)

  13. Differences and Similarities in Lawrence and Zhang Ailing’s Views on Female

    Institute of Scientific and Technical Information of China (English)

    冉一鸣

    2014-01-01

    D.H.Lawrence and Zhang Ailing both pay great attention to female in their novel writing. This thesis focus on the comparative study of their views on female. Through comparing their views, the author find the similarities and differences in them.

  14. Lawrence Livermore National Laboratory selects Intel Itanium 2 processors for world's most powerful Linux cluster

    CERN Multimedia

    2003-01-01

    "Intel Corporation, system manufacturer California Digital and the University of California at Lawrence Livermore National Laboratory (LLNL) today announced they are building one of the world's most powerful supercomputers. The supercomputer project, codenamed "Thunder," uses nearly 4,000 Intel® Itanium® 2 processors... is expected to be complete in January 2004" (1 page).

  15. A Theoretical Exploration of Lawrence of Arabia’s Inner Meanings on Guerrilla Warfare

    Science.gov (United States)

    2011-07-05

    reason to believe that, even more than Sun Tzu, Lawrence has for many years been discreetly plagiarized by Mao Zedong and his associates. 27 What...organized communications, for irregular war is fairly Willisen‟s definition of strategy, „the study of communication,‟ in its extreme degree, of

  16. William and Lawrence Bragg father and son the most extraordinary collaboration in science

    CERN Document Server

    Jenkin, John

    2008-01-01

    This joint biography is of William and Lawrence Bragg, who changed all of science in the 20th-century with the development of X-ray crystallography, and by mentoring the mid-century discovery of the structure of DNA. Their stories are vivid examples of science teaching and research in a colonial setting (Australia).

  17. Challenges in the miRNA research

    DEFF Research Database (Denmark)

    Singh, Tiratha Raj; Gupta, Arun; Suravajhala, Prashanth

    2013-01-01

    While it is known that the human genes are regulated by microRNAs (miRNAs), recent links with cancer and other diseases have widely caught interest. With several bioinformatics platforms and approaches on rise that has led to discovery of human miRNAs, validation and need for understanding miRNAs...

  18. Possible mechanisms of action of environmental contaminants on St. Lawrence beluga whales (Delphinapterus leucas).

    Science.gov (United States)

    De Guise, S; Martineau, D; Béland, P; Fournier, M

    1995-05-01

    A small isolated population of beluga whales (Delphinapterus leucas) that are highly contaminated by pollutants, mostly of industrial origin, resides in the St. Lawrence estuary, Québec, Canada. Overhunting in the first half of the century was the probable cause for this population to dwindle from several thousand animals to the current estimate of 500. The failure of the population to recover might be due to contamination by organochlorine compounds, which are known to lead to reproductive failure and immunosuppression in domestic and laboratory animals and seals. Functional and morphological changes have been demonstrated in thyroid gland and adrenal cortex in many species exposed to organochlorinated compounds, including seals. Morphological lesions, although different, were also found in belugas. Functional evaluation of thyroid and adrenal glands of contaminated (St. Lawrence) versus much less contaminated (Arctic) belugas is currently under way. Necropsy of St. Lawrence belugas showed numerous severe and disseminated infections with rather mildly pathogenic bacteria, which suggests immunosuppression. Organochlorine compounds and other contaminants found in beluga whales cause immunosuppression in a variety of animal species including seals. Thirty-seven percent of all the tumors reported in cetaceans were observed in St. Lawrence beluga whales. This could be explained by two different mechanisms: high exposure to environmental carcinogens and suppression of immunosurveillance against tumors. Overall, St. Lawrence belugas might well represent the risk associated with long-term exposure to pollutants present in their environment and might be a good model to predict health problems that could emerge in highly exposed human populations over time.

  19. miRNAs in brain development

    Energy Technology Data Exchange (ETDEWEB)

    Petri, Rebecca; Malmevik, Josephine; Fasching, Liana; Åkerblom, Malin; Jakobsson, Johan, E-mail: johan.jakobsson@med.lu.se

    2014-02-01

    MicroRNAs (miRNAs) are small, non-coding RNAs that negatively regulate gene expression at the post-transcriptional level. In the brain, a large number of miRNAs are expressed and there is a growing body of evidence demonstrating that miRNAs are essential for brain development and neuronal function. Conditional knockout studies of the core components in the miRNA biogenesis pathway, such as Dicer and DGCR8, have demonstrated a crucial role for miRNAs during the development of the central nervous system. Furthermore, mice deleted for specific miRNAs and miRNA-clusters demonstrate diverse functional roles for different miRNAs during the development of different brain structures. miRNAs have been proposed to regulate cellular functions such as differentiation, proliferation and fate-determination of neural progenitors. In this review we summarise the findings from recent studies that highlight the importance of miRNAs in brain development with a focus on the mouse model. We also discuss the technical limitations of current miRNA studies that still limit our understanding of this family of non-coding RNAs and propose the use of novel and refined technologies that are needed in order to fully determine the impact of specific miRNAs in brain development. - Highlights: • miRNAs are essential for brain development and neuronal function. • KO of Dicer is embryonically lethal. • Conditional Dicer KO results in defective proliferation or increased apoptosis. • KO of individual miRNAs or miRNA families is necessary to determine function.

  20. Challenges in the miRNA research

    DEFF Research Database (Denmark)

    Singh, Tiratha Raj; Gupta, Arun; Suravajhala, Prashanth

    2013-01-01

    RNAs from their progenitor messenger RNAs (mRNAs) have arisen. Furthermore, the miRNAs are known to have synergism involving regulation of their condition-specific target genes (mRNAs). In this review, we provide a bioinformatics approach of the miRNAs and their challenges with respect to annotation......While it is known that the human genes are regulated by microRNAs (miRNAs), recent links with cancer and other diseases have widely caught interest. With several bioinformatics platforms and approaches on rise that has led to discovery of human miRNAs, validation and need for understanding mi...

  1. Measurement of target and double-spin asymmetries for the <mi>e><mi>pmi><mi>emi><mimi>+(<mi>n>) reaction in the nucleon resonance region at low <mi>Q>2

    Energy Technology Data Exchange (ETDEWEB)

    Zheng, X.; Adhikari, K. P.; Bosted, P.; Deur, A.; Drozdov, V.; El Fassi, L.; Kang, Hyekoo; Kovacs, K.; Kuhn, S.; Long, E.; Phillips, S. K.; Ripani, M.; Slifer, K.; Smith, L. C.; Adikaram, D.; Akbar, Z.; Amaryan, M. J.; Anefalos Pereira, S.; Asryan, G.; Avakian, H.; Badui, R. A.; Ball, J.; Baltzell, N. A.; Battaglieri, M.; Batourine, V.; Bedlinskiy, I.; Biselli, A. S.; Briscoe, W. J.; Bültmann, S.; Burkert, V. D.; Carman, D. S.; Celentano, A.; Chandavar, S.; Charles, G.; Chen, J. -P.; Chetry, T.; Choi, Seonho; Ciullo, G.; Clark, L.; Colaneri, L.; Cole, P. L.; Compton, N.; Contalbrigo, M.; Crede, V.; D' Angelo, A.; Dashyan, N.; De Vita, R.; De Sanctis, E.; Djalali, C.; Dodge, G. E.; Dupre, R.; Egiyan, H.; El Alaoui, A.; Elouadrhiri, L.; Eugenio, P.; Fanchini, E.; Fedotov, G.; Fersch, R.; Filippi, A.; Fleming, J. A.; Gevorgyan, N.; Ghandilyan, Y.; Gilfoyle, G. P.; Giovanetti, K. L.; Girod, F. X.; Gleason, C.; Golovach, E.; Gothe, R. W.; Griffioen, K. A.; Guidal, M.; Guler, N.; Guo, L.; Hanretty, C.; Harrison, N.; Hattawy, M.; Hicks, K.; Holtrop, M.; Hughes, S. M.; Ilieva, Y.; Ireland, D. G.; Ishkhanov, B. S.; Isupov, E. L.; Jenkins, D.; Jiang, H.; Jo, H. S.; Joosten, S.; Keller, D.; Khachatryan, G.; Khandaker, M.; Kim, A.; Kim, W.; Klein, F. J.; Kubarovsky, V.; Lanza, L.; Lenisa, P.; Livingston, K.; MacGregor, I. J. D.; Markov, N.; McKinnon, B.; Mirazita, M.; Mokeev, V.; Movsisyan, A.; Munevar, E.; Munoz Camacho, C.; Murdoch, G.; Nadel-Turonski, P.; Net, L. A.; Ni, A.; Niccolai, S.; Niculescu, G.; Niculescu, I.; Osipenko, M.; Ostrovidov, A. I.; Paolone, M.; Paremuzyan, R.; Park, K.; Pasyuk, E.; Peng, P.; Pisano, S.; Pogorelko, O.; Price, J. W.; Puckett, A. J. R.; Raue, B. A.; Rizzo, A.; Rosner, G.; Rossi, P.; Roy, P.; Sabatié, F.; Salgado, C.; Schumacher, R. A.; Sharabian, Y. G.; Skorodumina, Iu.; Smith, G. D.; Sokhan, D.; Sparveris, N.; Stankovic, I.; Strakovsky, I. I.; Strauch, S.; Taiuti, M.; Tian, Ye; Ungaro, M.; Voskanyan, H.; Voutier, E.; Walford, N. K.; Watts, D. P.; Wei, X.; Weinstein, L. B.; Wood, M. H.; Zachariou, N.; Zhang, J.; Zonta, I.

    2016-10-01

    We report measurements of target- and double-spin asymmetries for the exclusive channel <mi>e><mi>pmi><mi>emi><mimi>+(<mi>n>) in the nucleon resonance region at Jefferson Lab using the CEBAF Large Acceptance Spectrometer (CLAS). These asymmetries were extracted from data obtained using a longitudinally polarized NH3 target and a longitudinally polarized electron beam with energies 1.1, 1.3, 2.0, 2.3, and 3.0 GeV. The new results are consistent with previous CLAS publications but are extended to a low Q2 range from 0.0065 to 0.35 (GeV/c)2. The Q2 access was made possible by a custom-built Cherenkov detector that allowed the detection of electrons for scattering angles as low as 6 degrees. These results are compared with the unitary isobar models JANR and MAID, the partial-wave analysis prediction from SAID, and the dynamic model DMT. In many kinematic regions our results, in particular results on the target asymmetry, help to constrain the polarization-dependent components of these models.

  2. Dollar Summary of Prime Contract Awards by State, County, Contractor and Place. Part 3 (Midland-Monroe)

    Science.gov (United States)

    1990-01-01

    co .(4 4N c- td o0-40 YV oC)wV oi C V) M0 in C2C N 0 La4 LID Co. 10 Ili " CO ---.- -& 0 .4C n0 0 CO4- 40 0 e CC SOwV 0 C In qt in. n 0 so cy I I 4 4 I...f*0 OI a* C N O (. I’ P. -4q n Y 1 1J cm P ONO Cn IsmlP .-4 .44O CV) P.O 10In Z0 o IIC C24 02 n r HZJ I W MI I.-I Iz I. U 0 0 -C-440 I. co 1.- WI00 0...an -4 COX CIO a- L-a N C’) C N a Z a C) C.= td ~ 4> I C’P a 4- ’ aCL OL. =2-e 20 0UaM 0a 0=C𔃾C r MC1 L -EEcEC.o ,Et a % 00 U a -(a a a a a)> 0 U a

  3. The regulatory epicenter of miRNAs

    Indian Academy of Sciences (India)

    Ashwani Jha; Mrigaya Mehra; Ravi Shankar

    2011-09-01

    miRNAs are small non-coding RNAs with average length of ∼21 bp. miRNA formation seems to be dependent upon multiple factors besides Drosha and Dicer, in a tissue/stage-specific manner, with interplay of several specific binding factors. In the present study, we have investigated transcription factor binding sites in and around the genomic sequences of precursor miRNAs and RNA-binding protein (RBP) sites in miRNA precursor sequences, analysed and tested in comprehensive manner. Here, we report that miRNA precursor regions are positionally enriched for binding of transcription factors as well as RBPs around the 3′ end of mature miRNA region in 5′ arm. The pattern and distribution of such regulatory sites appears to be a characteristic of precursor miRNA sequences when compared with non-miRNA sequences as negative dataset and tested statistically. When compared with 1 kb upstreamregions, a sudden sharp peak for binding sites arises in the enriched zone near the mature miRNA region. An expression-data-based correlation analysis was performed between such miRNAs and their corresponding transcription factors and RBPs for this region. Some specific groups of binding factors and associated miRNAs were identified. We also identified some of the overrepresented transcription factors and associated miRNAs with high expression correlation values which could be useful in cancer-related studies. The highly correlated groups were found to host experimentally validated composite regulatory modules, in which Lmo2-GATA1 appeared as the predominant one. For many of RBP–miRNAs associations, co-expression similarity was also evident among the associated miRNA common to given RBPs, supporting the Regulon model, suggesting a common role and common control of these miRNAs by the associated RBPs. Based on our findings, we propose that the observed characteristic distribution of regulatory sites in precursor miRNA sequence regions could be critical inmiRNA transcription, processing

  4. miRConnect:Identifying effector genes of miRNAs and miRNA families in cancer cells

    DEFF Research Database (Denmark)

    Hua, Youjia; Duan, Shiwei; Murmann, Andrea E

    2011-01-01

    micro(mi)RNAs are small non-coding RNAs that negatively regulate expression of most mRNAs. They are powerful regulators of various differentiation stages, and the expression of genes that either negatively or positively correlate with expressed miRNAs is expected to hold information on the biolog......micro(mi)RNAs are small non-coding RNAs that negatively regulate expression of most mRNAs. They are powerful regulators of various differentiation stages, and the expression of genes that either negatively or positively correlate with expressed miRNAs is expected to hold information...... have generated custom data sets containing expression information of 54 miRNA families sharing the same seed match. We have developed a novel strategy for correlating miRNAs with individual genes based on a summed Pearson Correlation Coefficient (sPCC) that mimics an in silico titration experiment...

  5. miRNA-based drought regulation in wheat.

    Science.gov (United States)

    Akdogan, Guray; Tufekci, Ebru Derelli; Uranbey, Serkan; Unver, Turgay

    2016-05-01

    MicroRNAs (miRNAs) are a class of small non-coding regulatory RNAs that regulate gene expression by guiding target mRNA cleavage or translational inhibition. Drought is a common environmental stress influencing crop growth and development. To date, it has been reported that a number of plant miRNA are involved in drought stress response. In this study, we comparatively investigated drought stress-responsive miRNAs in the root and leaf of bread wheat (Triticum aestivum cv. Sivas 111/33) by miRNA microarray screening. miRNA microarray analysis showed that 285 miRNAs (207 upregulated and 78 downregulated) and 244 miRNAs (115 upregulated and 129 downregulated) were differentially expressed in leaf and root tissues, respectively. Among the differentially expressed miRNAs, 23 miRNAs were only expressed in the leaf and 26 miRNAs were only expressed in the root of wheat growth under drought stress. Upon drought treatment, expression of miR159, miR160, miR166, miR169, miR172, miR395, miR396, miR408, miR472, miR477, miR482, miR1858, miR2118, and miR5049 were found to be significantly differentiated in bread wheat. The regulatory network analysis showed that miR395 has connections with a number of target transcripts, and miR159 and miR319 share a number of target genes. Drought-tolerant and drought-sensitive wheat cultivars showed altered expression pattern upon drought stress in terms of investigated miRNA and their target transcript expression level.

  6. Clinical results of stereotactic hellium-ion radiosurgery of the pituitary gland at Lawrence Berkeley Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Levy, R.P.; Fabrikant, J.I.; Lyman, J.T.; Frankel, K.A.; Phillips, M.H.; Lawrence, J.H.; Tobias, C.A.

    1989-12-01

    The first therapeutic clinical trial using accelerated heavy-charged particles in humans was performed at Lawrence Berkeley Laboratory (LBL) for the treatment of various endocrine and metabolic disorders of the pituitary gland, and as suppressive therapy for adenohypophyseal hormone-responsive carcinomas and diabetic retinopathy. In acromegaly, Cushing's disease, Nelson's syndrome and prolactin-secreting tumors, the therapeutic goal in the 433 patients treated has been to destroy or inhibit the growth of the pituitary tumor and control hormonal hypersecretion, while preserving a functional rim of tissue with normal hormone-secreting capacity, and minimizing neurologic injury. An additional group of 34 patients was treated for nonsecreting chromophobe adenomas. This paper discusses the methods and results of stereotactic helium-ion radiosurgery of the pituitary gland at Lawrence Berkeley Laboratory. 11 refs.

  7. Shark predation on migrating adult american eels (Anguilla rostrata) in the Gulf of St. Lawrence

    DEFF Research Database (Denmark)

    Béguer-Pon, Mélanie; Benchetrit, José; Castonguay, Martin;

    2012-01-01

    to identify the eel predators, we compared their vertical migratory behavior with those of satellite-tagged porbeagle shark and bluefin tuna, the only endothermic fishes occurring non-marginally in the Gulf of St. Lawrence. We accurately distinguished between tuna and shark by using the behavioral criteria...... generated by comparing the diving behavior of these two species with those of our unknown predators. Depth profile characteristics of most eel predators more closely resembled those of sharks than those of tuna. During the first days following tagging, all eels remained in surface waters and did not exhibit...... itself may contribute to increasing the eel's susceptibility to predation, we discuss evidence suggesting that predation of silver-stage American eels by porbeagle sharks may represent a significant source of mortality inside the Gulf of St. Lawrence and raises the possibility that eels may represent...

  8. A one-dimensional, steady-state, dissolved-oxygen model and waste-load assimilation study for Clear Creek, Monroe County, Indiana

    Science.gov (United States)

    Wilber, William G.; Crawford, Charles G.; Peters, J.G.; Girardi, F.P.

    1979-01-01

    A digital model calibrated to conditions in Clear Creek, Monroe County, IN, was used to develop alternatives for future waste loadings that would be compatible with Indiana stream water-quality standards defined for two critical hydrologic conditions, summer and winter low flows. The Winston Thomas wastewater-treatment facility is the only point-source waste load affecting the modeled reach of Clear Creek. A new waste-water-treatment facility under construction at Dillman Road (river mile 13.78) will replace the Winston Thomas wastewater-treatment facility (river mile 16.96) in 1980. Natural streamflow during the summer and annual 7-day, 10-year low flow is zero, so no benefit from dilution is provided. The model indicates that ammonia-nitrogen toxicity is the most significant factor affecting the stream water quality during summer and winter low flows. The ammonia-nitrogen concentration of the wastewater effluent exceeds the maximum total ammonia-nitrogen concentration of 2.5 milligrams per liter for summer months (June through August) and 4.0 milligrams per liter for winter months (November through March) required for Indiana streams. Nitrification, benthic-oxygen demand, and algal respiration were the most significant factors affecting the dissolved-oxygen concentration in Clear Creek during the model calibration. Nitrification should not significantly affect the dissolved-oxygen concentration in Clear Creek during summer low flows when the ammonia-nitrogen toxicity standards are met. (USGS)

  9. Cardio-miRNAs and onco-miRNAs: circulating miRNA-based diagnostics for non-cancerous and cancerous diseases

    Directory of Open Access Journals (Sweden)

    Masaru eKatoh

    2014-10-01

    Full Text Available Cardiovascular diseases and cancers are the leading causes of morbidity and mortality in the world. MicroRNAs (miRNAs are short non-coding RNAs that primarily repress target mRNAs. Here, miR-24, miR-125b, miR-195 and miR-214 were selected as representative cardio-miRs that are upregulated in human heart failure. To bridge the gap between miRNA studies in cardiology and oncology, the targets and functions of these miRNAs in cardiovascular diseases and cancers will be reviewed. ACVR1B, BCL2, BIM, eNOS, FGFR3, JPH2, MEN1, MYC, p16 and ST7L are miR-24 targets that have been experimentally validated in human cells. ARID3B, BAK1, BCL2, BMPR1B, ERBB2, FGFR2, IL6R, MUC1, SITR7, Smoothened, STAT3, TET2 and TP53 are representative miR-125b targets. ACVR2A, BCL2, CCND1, E2F3, GLUT3, MYB, RAF1, VEGF, WEE1 and WNT7A are representative miR-195 targets. BCL2L2, ß-catenin, BIM, CADM1, EZH2, FGFR1, NRAS, PTEN, TP53 and TWIST1 are representative miR-214 targets. miR-125b is a good cardio-miR that protects cardiomyocytes; miR-195 is a bad cardio-miR that elicits cardiomyopathy and heart failure; miR-24 and miR-214 are bi-functional cardio-miRs. By contrast, miR-24, miR-125b, miR-195 and miR-214 function as oncogenic or tumor suppressor miRNAs in a cancer (subtype-dependent manner. Circulating miR-24 is elevated in diabetes, breast cancer and lung cancer. Circulating miR-195 is elevated in acute myocardial infarction, breast cancer, prostate cancer and colorectal adenoma. Circulating miR-125b and miR-214 are elevated in some cancers. Cardio-miRs and onco-miRs bear some similarities in functions and circulation profiles. Because circulating miRNA profiles are modulated by genetic and environmental factors and are dysregulated by genetic and epigenetic alterations in somatic cells, circulating miRNA association studies (CMASs within several thousands of cases each for common non-cancerous diseases and major cancers are necessary for miRNA-based diagnostics.

  10. Fire History of Appalachian Forests of the Lower St-Lawrence Region (Southern Quebec)

    OpenAIRE

    Serge Payette; Vanessa Pilon; Pierre-Luc Couillard; Jason Laflamme

    2017-01-01

    Sugar maple (Acer saccharum) forests are among the main forest types of eastern North America. Sugar maple stands growing on Appalachian soils of the Lower St-Lawrence region are located at the northeastern limit of the northern hardwood forest zone. Given the biogeographical position of these forests at the edge of the boreal biome, we aimed to reconstruct the fire history and document the occurrence of temperate and boreal trees in sugar maple sites during the Holocene based on soil macroch...

  11. Possible mechanisms of action of environmental contaminants on St. Lawrence beluga whales (Delphinapterus leucas).

    OpenAIRE

    de Guise, S.; Martineau, D.; Béland, P; Fournier, M.

    1995-01-01

    A small isolated population of beluga whales (Delphinapterus leucas) that are highly contaminated by pollutants, mostly of industrial origin, resides in the St. Lawrence estuary, Québec, Canada. Overhunting in the first half of the century was the probable cause for this population to dwindle from several thousand animals to the current estimate of 500. The failure of the population to recover might be due to contamination by organochlorine compounds, which are known to lead to reproductive f...

  12. Dermatophilus-like infection in beluga whales, Delphinapterus leucas, from the St. Lawrence estuary.

    Science.gov (United States)

    Mikaelian, I; Lapointe, J M; Labelle, P; Higgins, R; Paradis, M; Martineau, D

    2001-02-01

    Six beluga whales (Delphinapterus leucas) found dead on the shores of the St. Lawrence estuary had multiple slightly depressed greyish round areas randomly distributed over the whole body. Histologically, the surface of these areas was covered with a thick layer of Dermatophilus-like organisms which invaded the stratum corneum. The underlying stratum spinosum had marked spongiosis and vacuolar degeneration. Minimal neutrophilic infiltration was present within the underlying dermal papillae. To the authors' knowledge, dermatophilosis in cetaceans has not been reported previously.

  13. Development of a Novel Depleted Uranium Treatment Process at Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Gates-Anderson, D; Bowers, J; Laue, C; Fitch, T

    2007-01-22

    A three-stage process was developed at Lawrence Livermore National Laboratory to treat potentially pyrophoric depleted uranium metal wastes. The three-stage process includes waste sorting/rinsing, acid dissolution of the waste metal with a hydrochloric and phosphoric acid solution, and solidification of the neutralized residuals from the second stage with clay. The final product is a solid waste form that can be transported to and disposed of at a permitted low-level radioactive waste disposal site.

  14. 2003 Lawrence Livermore National Laboratory Annual Illness and Injury Surveillance Report

    Energy Technology Data Exchange (ETDEWEB)

    U.S. Department of Energy, Office of Health, Safety and Security, Office of Illness and Injury Prevention Programs

    2007-05-23

    Annual Illness and Injury Surveillance Program report for 2003 for Lawrence Livermore National Lab. The U.S. Department of Energy’s (DOE) commitment to assuring the health and safety of its workers includes the conduct of epidemiologic surveillance activities that provide an early warning system for health problems among workers. The IISP monitors illnesses and health conditions that result in an absence of workdays, occupational injuries and illnesses, and disabilities and deaths among current workers.

  15. Tertiary structure mapping of the pri-miRNA miR-17~92.

    Science.gov (United States)

    Chaulk, Steven G; Fahlman, Richard P

    2014-01-01

    The understanding of RNA in regulating gene expression has exploded over the past 15 years. MicroRNAs (miRNAs) have vastly expanded the role of RNA in gene regulation beyond spliceosomal, ribosomal, and messenger RNAs. Approximately one half of miRNAs are polycistronic, where two or more miRNAs are encoded on a single pri-miRNA transcript, termed a miRNA cluster. The six miRNAs of the miR-17~92 cluster are contained within a ~800 nucleotide region within intron 3 of the cl13orf25 ~7 kb pri-miRNA transcript. We recently reported on the tertiary structured domain of miR-17~92 and its role in modulating miRNA biogenesis. The key finding was that the cluster structure explained the differential processing of the miRNA hairpins by Drosha. This work demonstrated the need to consider pri-miRNA tertiary structure in miRNA biogenesis. Since biochemical structure probing is typically performed on relatively short RNAs (≤200 nucleotides), we had to adapt these methodologies for application on large RNAs (~800 nucleotide miR-17~92 pri-miRNA). We present here our adaptation of a protection footprinting method using ribonucleases to probe the structure of the ~800 nucleotide miR-17~92 pri-miRNA. We outline the technical difficulties involved in probing large RNAs and data visualization using denaturing polyacrylamide gel electrophoresis and how we adapted the existing approaches to probe large RNAs. The methodology outlined here is generally applicable to large RNAs including long noncoding RNAs (lncRNA).

  16. Indication of a Lombard vocal response in the St. Lawrence River beluga

    Science.gov (United States)

    Scheifele, P. M.; Andrew, S.; Cooper, R. A.; Darre, M.; Musiek, F. E.; Max, L.

    2005-03-01

    Noise pollution is recognized as a potential danger to marine mammals in general, and to the St. Lawrence beluga in particular. One method of determining the impacts of noise on an animal's communication is to observe a natural and repeatable response of the vocal system to variations in noise level. This is accomplished by observing intensity changes in animal vocalizations in response to environmental noise. One such response observed in humans, songbirds, and some primates is the Lombard vocal response. This response represents a vocal system reaction manifested by changes in vocalization level in direct response to changes in the noise field. In this research, a population of belugas in the St. Lawrence River Estuary was tested to determine whether a Lombard response existed by using hidden Markhov-classified vocalizations as targets for acoustical analyses. Correlation and regression analyses of signals and noise indicated that the phenomenon does exist. Further, results of human subjects experiments [Egan, J. J. (1966), Ph.D. dissertation; Scheifele, P. M. (2003), Ph.D. dissertation], along with previously reported data from other animal species, are similar to those exhibited by the belugas. Overall, findings suggest that typical noise levels in the St. Lawrence River Estuary have a detectable effect on the communication of the beluga. .

  17. Diagnostic potential of miR-126, miR-143, miR-145, and miR-652 in malignant pleural mesothelioma

    DEFF Research Database (Denmark)

    Andersen, Morten; Grauslund, Morten; Ravn, Jesper;

    2014-01-01

    RNAs in formalin-fixed, paraffin-embedded, preoperative diagnostic biopsy samples, surgically resected MPM specimens previously treated with chemotherapy, and corresponding non-neoplastic pleura (NNP), from five patients. miR-126, miR-143, miR-145, and miR-652 were significantly down-regulated (≥twofold.......88-1.00). The level of miR-126 in MPM was inversely correlated with that of the known target, the large neutral amino acid transporter, small subunit 1 (r = -0.38; 95% CI, -0.63 to -0.06). Overall, these results indicate that these four miRNAs may be suitable biomarkers for distinguishing MPM from RMPs....

  18. The miRNome of bipolar disorder.

    Science.gov (United States)

    Fries, Gabriel R; Carvalho, Andre F; Quevedo, Joao

    2017-09-23

    Epigenetic mechanisms have been suggested to play a key role in the pathophysiology of bipolar disorder (BD), among which microRNAs (miRNAs) may be of particular significance according to recent studies. We aimed to summarize miRNA studies in BD to identify consistent findings, limitations, and future directions of this emerging field. We performed a comprehensive search on PUBMED and Medline for studies investigating an association between BD and miRNAs. The included studies report miRNA alterations in postmortem brain tissues and in the periphery, cell culture and preclinical findings, genetic associations, and the effects of medications. Several studies report changes in miRNA expression levels in postmortem brain and in the periphery of patients, although most of the results so far have not been replicated and are not concordant between different populations. Genetic studies also suggest that miRNA genes are located within susceptibility loci of BD, and also a putative role of miRNAs in modulating genes previously shown to confer risk of BD. We did not perform a systematic review of the literature, and miRNAs represent only one facet of the plethora of epigenetic mechanisms that might be involved in BD's pathophysiology. miRNA findings in BD significantly vary between studies, but are consistent to suggest a key role for these molecules in BD's pathophysiology and treatment, particularly miR-34a and miR-137. Accordingly, miRNA might represent important biomarkers of illness to be used in the clinical settings, and potentially also for the development of novel therapeutics for BD in the near future. Copyright © 2017 Elsevier B.V. All rights reserved.

  19. Circulating miRNA and cancer diagnosis

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    miRNAs are a class of small RNA molecules with regulatory function, and play an important role in tumor development and progression. It has been demonstrated that tumor-derived miRNAs exist in the circulating nucleic acids of cancer patients. This phenomenon implies that detection of the circulating miRNA may be an effective method for non-invasive diagnosis of cancer. In this review, we summarize the applications of the circulating miRNA as biomarkers in cancer diagnosis, as well as the latest research progress in this area.

  20. Racism and cultural identity: the reflections of two Black trainee teachers' engagement with the Stephen Lawrence Symposium

    OpenAIRE

    Edwards, Martyn

    2015-01-01

    The Stephen Lawrence Symposium held in London in 2013 provided an opportunity for academics and educators to reflect on changes in education and wider society resulting from the legacy of Stephen Lawrence over the 20 years since his racist murder. A Black African trainee teacher and a Black Caribbean trainee teacher in post-16 teacher training at a large university in the North of England participated in a series of lunchtime discussion groups as part of their university-based training. This ...

  1. miRTex: A Text Mining System for miRNA-Gene Relation Extraction.

    Science.gov (United States)

    Li, Gang; Ross, Karen E; Arighi, Cecilia N; Peng, Yifan; Wu, Cathy H; Vijay-Shanker, K

    2015-01-01

    MicroRNAs (miRNAs) regulate a wide range of cellular and developmental processes through gene expression suppression or mRNA degradation. Experimentally validated miRNA gene targets are often reported in the literature. In this paper, we describe miRTex, a text mining system that extracts miRNA-target relations, as well as miRNA-gene and gene-miRNA regulation relations. The system achieves good precision and recall when evaluated on a literature corpus of 150 abstracts with F-scores close to 0.90 on the three different types of relations. We conducted full-scale text mining using miRTex to process all the Medline abstracts and all the full-length articles in the PubMed Central Open Access Subset. The results for all the Medline abstracts are stored in a database for interactive query and file download via the website at http://proteininformationresource.org/mirtex. Using miRTex, we identified genes potentially regulated by miRNAs in Triple Negative Breast Cancer, as well as miRNA-gene relations that, in conjunction with kinase-substrate relations, regulate the response to abiotic stress in Arabidopsis thaliana. These two use cases demonstrate the usefulness of miRTex text mining in the analysis of miRNA-regulated biological processes.

  2. Evolutionary and Expression Analysis of miR-#-5p and miR-#-3p at the miRNAs/isomiRs Levels

    Directory of Open Access Journals (Sweden)

    Li Guo

    2015-01-01

    Full Text Available We mainly discussed miR-#-5p and miR-#-3p under three aspects: (1 primary evolutionary analysis of human miRNAs; (2 evolutionary analysis of miRNAs from different arms across the typical 10 vertebrates; (3 expression pattern analysis of miRNAs at the miRNA/isomiR levels using public small RNA sequencing datasets. We found that no bias can be detected between the numbers of 5p-miRNA and 3p-miRNA, while miRNAs from miR-#-5p and miR-#-3p show variable nucleotide compositions. IsomiR expression profiles from the two arms are always stable, but isomiR expressions in diseased samples are prone to show larger degree of dispersion. miR-#-5p and miR-#-3p have relative independent evolution/expression patterns and datasets of target mRNAs, which might also contribute to the phenomena of arm selection and/or arm switching. Simultaneously, miRNA/isomiR expression profiles may be regulated via arm selection and/or arm switching, and the dynamic miRNAome and isomiRome will adapt to functional and/or evolutionary pressures. A comprehensive analysis and further experimental study at the miRNA/isomiR levels are quite necessary for miRNA study.

  3. miRNA-mediated deregulation in leukemia

    Directory of Open Access Journals (Sweden)

    Carmela eDell'Aversana

    2012-11-01

    Full Text Available MicroRNAs (miRNAs are small non-coding RNAs 18 to 22 nucleotides (nt long able to fine-tune post-transcriptional gene expression. Extensive investigation intobiogenesis, mechanism of action and functions of miRNAs has clearly revealed their prompt control in developmental timing, differentiation, proliferation, cell death and metabolism. Deregulationof miRNA-mediated pathways may contribute to pathological conditions such as tumors, including hematological cancer, thus suggesting that miRNAs act both as tumor-suppressor genes (TSG and oncogenes (OG.Here, we provide an overview of the current understanding of the aberration of miRNA biogenesis, activity and post-transcriptional control in leukemogenesis.

  4. MiRNA Biogenesis and Intersecting Pathways

    DEFF Research Database (Denmark)

    Ben Chaabane, Samir

    MicroRNAs (miRNAs) are small non-coding RNAs that function as guide molecules in RNA silencing. Plant miRNAs are critical for plant growth, development and stress response, and are processed in Arabidopsis from primary miRNA transcripts (pri-miRNAs) by the endonuclease activity of the DICER-LIKE1...... of action and turnover. During my PhD period we have shown that the STA1 protein, a factor for pre-mRNA splicing and mRNA stability, is specifically involved in the splicing of pri-miRNAs and in the modulation of DCL1 transcript levels. Also, we established a novel and essential regulatory network in which...

  5. miRNA deregulation in multiple myeloma

    Institute of Scientific and Technical Information of China (English)

    BI Chong Lei; CHNG Wee Joo

    2011-01-01

    Multiple myeloma (MM) is an incurable plasma cell malignancy and is the second most common hematological cancer. It is characterized by complex, recurrent genetic and epigenetic abnormalities. Recent publications have linked miRNAs, a novel class of gene regulators to cancer including MM. miRNAs are about 20 nucleotide, single strand, non-coding RNAs that repress gene expression by mRNA degradation or translational repression. Aberrant miRNA expression profiles have been described in MM, and their functional roles in MM pathogenesis are being increasingly recognized. This review summarizes the current literature on the role of miRNAs in MM and offers perspectives on future research and utilization of miRNAs in MM management.

  6. Shark predation on migrating adult American eels (Anguilla rostrata in the Gulf of St. Lawrence.

    Directory of Open Access Journals (Sweden)

    Mélanie Béguer-Pon

    Full Text Available In an attempt to document the migratory pathways and the environmental conditions encountered by American eels during their oceanic migration to the Sargasso Sea, we tagged eight silver eels with miniature satellite pop-up tags during their migration from the St. Lawrence River in Québec, Canada. Surprisingly, of the seven tags that successfully transmitted archived data, six were ingested by warm-gutted predators, as observed by a sudden increase in water temperature. Gut temperatures were in the range of 20 to 25°C-too cold for marine mammals but within the range of endothermic fish. In order to identify the eel predators, we compared their vertical migratory behavior with those of satellite-tagged porbeagle shark and bluefin tuna, the only endothermic fishes occurring non-marginally in the Gulf of St. Lawrence. We accurately distinguished between tuna and shark by using the behavioral criteria generated by comparing the diving behavior of these two species with those of our unknown predators. Depth profile characteristics of most eel predators more closely resembled those of sharks than those of tuna. During the first days following tagging, all eels remained in surface waters and did not exhibit diel vertical migrations. Three eels were eaten at this time. Two eels exhibited inverse diel vertical migrations (at surface during the day during several days prior to predation. Four eels were eaten during daytime, whereas the two night-predation events occurred at full moon. Although tagging itself may contribute to increasing the eel's susceptibility to predation, we discuss evidence suggesting that predation of silver-stage American eels by porbeagle sharks may represent a significant source of mortality inside the Gulf of St. Lawrence and raises the possibility that eels may represent a reliable, predictable food resource for porbeagle sharks.

  7. Mi oíslo

    Directory of Open Access Journals (Sweden)

    Mitja Skubic

    2006-12-01

    Full Text Available Hablando Sancho en su primer encuentro con don Quijote de su mujer Juana Gutiérrez, llamada en seguida Teresa Panza o simplemente Teresa, la nombra mi oíslo y repite lo mismo otras dos veces. Conviene tener presente que de su mujer Sancho habla siempre respetuosamente y con mucho cariño, con excepción de una conversación que llega a ser un litigio, II, 5. Allí, Sancho expresa su idea de cómo y con quién casar a la hija Sanchica y por fin impone su voluntad. Teresa se le opone vigorosamente y esto induce a Sancho, sobreexcitado, a formular una gradación sorprendente: mujer mía; mirad, Teresa; mujer; calla, boba; bestia y mujer de Barrabás; animalia; mentecata e ignorante.

  8. Vasopressin-regulated miRNAs and AQP2-targeting miRNAs in kidney collecting duct cells.

    Science.gov (United States)

    Kim, Jae-Eun; Jung, Hyun Jun; Lee, Yu-Jung; Kwon, Tae-Hwan

    2015-04-01

    Mature microRNA (miRNA) acts as an important posttranscriptional regulator. We aimed to profile vasopressin-responsive miRNAs in kidney inner medullary collecting duct cells and to identify aquaporin-2 (AQP2)-targeting miRNAs. Microarray chip assay was carried out in inner medullary collecting duct tubule suspensions from rat kidneys in the absence or presence of desmopressin (dDAVP) stimulation (10(-9) M, 2 h). The results demonstrated 19 miRNAs, including both precursor and mature miRNAs, as potential candidates that showed significant changes in expression after dDAVP stimulation (P 1.3-fold changes in expression on the microarray (miR-127, miR-1, miR-873, miR-16, miR-206, miR-678, miR-496, miR-298, and miR-463) were further examined by quantitative real-time PCR, and target genes of the selected miRNAs were predicted. Next, to identify AQP2-targeting miRNAs, in silico analysis was performed. Four miRNAs (miR-32, miR-137, miR-216a, and miR-216b) target the 3'-untranslated region of rat AQP2 mRNA. Target seed regions of miR-32 and miR-137 were also conserved in the 3'-untranslated region of mouse AQP2 mRNA. Quantitative real-time PCR and immunoblot analysis demonstrated that dDAVP-induced AQP2 expression was significantly attenuated in mpkCCDc14 cells when cells were transfected with miRNA mimics of miR-32 or miR-137. Moreover, luciferase reporter assay demonstrated a significant decrease of AQP2 translation in mpkCCDc14 cells transfected with miRNA mimics of miR-32 or miR-137. The present study provides novel insights into the regulation of AQP2 by RNA interference; however, vasopressin-regulated miRNAs did not include miR-32 or miR-137, indicating that the interaction of miRNAs with the AQP2 regulatory pathway requires further analysis. Copyright © 2015 the American Physiological Society.

  9. Signal and Image Processing Research at the Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Roberts, R S; Poyneer, L A; Kegelmeyer, L M; Carrano, C J; Chambers, D H; Candy, J V

    2009-06-29

    Lawrence Livermore National Laboratory is a large, multidisciplinary institution that conducts fundamental and applied research in the physical sciences. Research programs at the Laboratory run the gamut from theoretical investigations, to modeling and simulation, to validation through experiment. Over the years, the Laboratory has developed a substantial research component in the areas of signal and image processing to support these activities. This paper surveys some of the current research in signal and image processing at the Laboratory. Of necessity, the paper does not delve deeply into any one research area, but an extensive citation list is provided for further study of the topics presented.

  10. Lawrence Livermore National Laboratory Response to the Federal Communication Commission Notice of Proposed Rulemaking

    Energy Technology Data Exchange (ETDEWEB)

    Dowls, F

    2000-08-01

    Lawrence Livermore National Laboratory (LLNL) has been conducting UWB research for the past few decades and submits a general comments section and a paragraph by paragraph response for consideration. In general, the proposed rules look very sound and encouraging for promoting use of the spectrum by both government and commercial users. General comments include recommending minimum frequency spreading specification, possibly clarifying the peak power limits and keeping the high frequency limit as high as possible. LLNL finds that minimum interference occurs by keeping wideband wide and narrowband narrow and keeping the middle ground clear.

  11. Design of an information system for the Security Department of Lawrence Livermore Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Reid, W.R.

    1978-12-01

    The main objective of this project is to show the development and design of an information system to meet the needs and requirements of the Security Department of Lawrence Livermore Laboratory (LLL). The information system is designed to use data collected by the CAIN Access Control System and to provide Security with reliable and useful reports. These reports are designed to increase the efficiency of the Security Department in performing its functions as well as to automate several manual procedures. The project design is created to be implemented using computer facilities available at LLL and adhering to standards of the Data Processing Services Department.

  12. Exploratory Research and Development Fund, FY 1990. Report on Lawrence Berkeley Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    1992-05-01

    The Lawrence Berkeley Laboratory Exploratory R&D Fund FY 1990 report is compiled from annual reports submitted by principal investigators following the close of the fiscal year. This report describes the projects supported and summarizes their accomplishments. It constitutes a part of an Exploratory R&D Fund (ERF) planning and documentation process that includes an annual planning cycle, projection selection, implementation, and review. The research areas covered in this report are: Accelerator and fusion research; applied science; cell and molecular biology; chemical biodynamics; chemical sciences; earth sciences; engineering; information and computing sciences; materials sciences; nuclear science; physics and research medicine and radiation biophysics.

  13. Signal and Image Processing Research at the Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Roberts, R S; Poyneer, L A; Kegelmeyer, L M; Carrano, C J; Chambers, D H; Candy, J V

    2009-06-29

    Lawrence Livermore National Laboratory is a large, multidisciplinary institution that conducts fundamental and applied research in the physical sciences. Research programs at the Laboratory run the gamut from theoretical investigations, to modeling and simulation, to validation through experiment. Over the years, the Laboratory has developed a substantial research component in the areas of signal and image processing to support these activities. This paper surveys some of the current research in signal and image processing at the Laboratory. Of necessity, the paper does not delve deeply into any one research area, but an extensive citation list is provided for further study of the topics presented.

  14. Associated Western Universities summer participant program at the Lawrence Livermore National Laboratory, Summer 1997

    Energy Technology Data Exchange (ETDEWEB)

    Williams, B.

    1997-08-01

    The Associated Western Universities, Inc. (AWU) supports a student summer program at Lawrence Livermore National Laboratory (LLNL). This program is structured so that honors undergraduate students may participate in the Laboratory`s research program under direct supervision of senior Laboratory scientists. Included in this report is a list of the AWU participants for the summer of 1997. All students are required to submit original reports of their summer activities in a format of their own choosing. These unaltered student reports constitute the major portion of this report.

  15. Evaluation and recommendations on U. C. Lawrence Livermore Labortory Quality Assurance Program

    Energy Technology Data Exchange (ETDEWEB)

    Carpenter, F.D.; Horner, M.H.

    1978-04-12

    A study was conducted of the University of California's Lawrence Livermore Laboratory Quality Assurance Program, which focused on training needs and recommendations tailored to the various on-going programs. Specific attention was directed to an assessment of the quality status for the MFTF facility and the capabilities of assigned quality project engineers. Conclusions and recommendations are presented which not only address the purpose of this study, but extend into other areas to provide insight and needs for a total cost effective application of a quality assurance program.

  16. Visibility of St Lawrence belugas to aerial photography, estimated by direct observation

    OpenAIRE

    Michael CS Kingsley; Isabelle Gauthier

    2002-01-01

    The depleted population of belugas (Delphinapterus leucas) inhabiting the St Lawrence estuary, Canada, was monitored by periodic photographic aerial surveys. In order to correct counts made on aerial survey film and to obtain an estimate of the true size of the population, the diving behaviour and the visibility from the air of these animals was studied. A Secchi-disk turbidity survey in the belugas’ summer range showed that water clarity varied between 1.5 m and 11.6 m. By studying aerial ph...

  17. Groundwater quality in the Delaware and St. Lawrence River Basins, New York, 2010

    Science.gov (United States)

    Nystrom, Elizabeth A.

    2012-01-01

    Water samples were collected from 10 production and domestic wells in the Delaware River Basin in New York and from 20 production and domestic wells in the St. Lawrence River Basin in New York from August through November 2010 to characterize groundwater quality in the basins. The samples were collected and processed by standard U.S. Geological Survey procedures and were analyzed for 147 physiochemical properties and constituents, including major ions, nutrients, trace elements, pesticides, volatile organic compounds (VOCs), radionuclides, and indicator bacteria.

  18. Greenspan, Vasey and Worthen : D.H. Lawrence: Studies in Classic American Literature

    Directory of Open Access Journals (Sweden)

    Neil ROBERTS

    2004-10-01

    Full Text Available This book is, among other things, a reminder of how large a part chance plays in the emergence of classic works of literature. The book that was eventually published as Studies in Classic American Literature in 1923 was the outcome of a longer period of  gestation and drafting than any book of Lawrence’s other than Women in Love—about seven years in all. For some of that time, at least, Lawrence considered it (or one of the forms that it passed through as his most important work after The Ra...

  19. Public census data on CD-ROM at Lawrence Berkeley Laboratory. Revision 1

    Energy Technology Data Exchange (ETDEWEB)

    Merrill, D.W.

    1992-07-02

    In connection with the Comprehensive Epidemiologic Data Resource (CEDR) and Populations at Risk to Environmental Pollution (PAREP) projects, of the Information and Computing Sciences Division (ICSD) at Lawrence Berkeley Laboratory (LBL), are using public socioeconomic and geographic data files which are available to CEDR and PAREP collaborators via LBL`s computing network. At this time 67 CD-ROM diskettes (approximately 35 gigabytes) are on line via the Unix file server cedrcd.lbl.gov. Most of the files are from the US Bureau of the Census, and most pertain to the 1990 Census of Population and Housing. This paper contains a list of the CD-ROMs available.

  20. Public census data on CD-ROM at Lawrence Berkeley Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Merrill, D.W.

    1992-07-02

    In connection with the Comprehensive Epidemiologic Data Resource (CEDR) and Populations at Risk to Environmental Pollution (PAREP) projects, of the Information and Computing Sciences Division (ICSD) at Lawrence Berkeley Laboratory (LBL), are using public socioeconomic and geographic data files which are available to CEDR and PAREP collaborators via LBL's computing network. At this time 67 CD-ROM diskettes (approximately 35 gigabytes) are on line via the Unix file server cedrcd.lbl.gov. Most of the files are from the US Bureau of the Census, and most pertain to the 1990 Census of Population and Housing. This paper contains a list of the CD-ROMs available.

  1. Lawrence de Arabia narrada desde la pauta de sus fundidos encadenados

    OpenAIRE

    Roldán Castro, Ismael

    1999-01-01

    Existen diferentes métodos para la lectura rápida de textos escritos; sin embargo, está escasamente analizada la investigación que un método similar pueda ofrecer para la lectura de las producciones audiovisuales y películas cinematográficas. Partiendo de la consideración de las unidades de información fílmica como cada uno de los fundidos encadenados, se presenta el estudio realizado sobre la película: «Lawrence de Arabia» de David Lean, con unas interesantes conclusiones.

  2. miRNA array screening reveals cooperative MGMT-regulation between miR-181d-5p and miR-409-3p in glioblastoma.

    Science.gov (United States)

    Khalil, Susanna; Fabbri, Enrica; Santangelo, Alessandra; Bezzerri, Valentino; Cantù, Cinzia; Di Gennaro, Gianfranco; Finotti, Alessia; Ghimenton, Claudio; Eccher, Albino; Dechecchi, Maria; Scarpa, Aldo; Hirshman, Brian; Chen, Clark; Ferracin, Manuela; Negrini, Massimo; Gambari, Roberto; Cabrini, Giulio

    2016-05-10

    The levels of expression of O6-methylguanine-DNA methyltransferase (MGMT) are relevant in predicting the response to the alkylating chemotherapy in patients affected by glioblastoma. MGMT promoter methylation and the published MGMT regulating microRNAs (miRNAs) do not completely explain the expression pattern of MGMT in clinical glioblastoma specimens. Here we used a genome-wide microarray-based approach to identify MGMT regulating miRNAs. Our screen unveiled three novel MGMT regulating miRNAs, miR-127-3p, miR-409-3p, and miR-124-3p, in addition to the previously identified miR-181d-5p. Transfection of these three novel miRNAs into the T98G glioblastoma cell line suppressed MGMT mRNA and protein expression. However, their MGMT- suppressive effects are 30-50% relative that seen with miR-181d-5p transfection. In silico analyses of The Cancer Genome Atlas (TCGA) and Chinese Glioma Genome Atlas (CGGA) revealed that miR-181d-5p is the only miRNA that consistently exhibited inverse correlation with MGMT mRNA expression. However, statistical models incorporating both miR-181d-5p and miR-409-3p expression better predict MGMT expression relative to models involving either miRNA alone. Our results confirmed miR-181d-5p as the key MGMT-regulating miRNA. Other MGMT regulating miRNAs, including the miR-409-3p identified in this report, modify the effect of miR-181d-5p on MGMT expression. MGMT expression is, thus, regulated by cooperative interaction between key MGMT-regulating miRNAs.

  3. Expression of serum microRNAs( miR-222,miR-181,miR-216 ) in human hepatocellular carcinoma and its clinical significance

    Institute of Scientific and Technical Information of China (English)

    占美晓

    2013-01-01

    Objective To investigate the abnormal expression of microRNAs (miR-216,miR-222,miR-181) in the serum of patients with hepatocellular carcinoma (HCC) and its clinical significance.Methods Serum miRNAs expression was investigated in 49 patients with HCC and 25healthy normal controls by using real-time PCR technique,and then correlations between miRNAs expression

  4. A novel panel of serum miR-21/miR-155/miR-365 as a potential diagnostic biomarker for breast cancer

    Science.gov (United States)

    Han, Ji-Guang; Jiang, Yong-Dong; Zhang, Chun-Hui; Yang, Yan-Mei; Pang, Da; Song, Yan-Ni

    2017-01-01

    Purpose Insufficient sensitivity and specificity prevent the use of most existing biomarkers for early detection of breast cancer. Recently, it was reported that serum microRNAs (miRNAs) may be potential biomarkers in many cancer diseases. In this study, we investigated whether serum levels of 5 miRNAs including miR-21, miR-125b, miR-145, miR-155, and miR-365 could discriminate breast cancer patients and healthy controls. Methods Serum levels of miRNAs were measured by using quantitative real-time polymerase chain reaction in 99 breast cancer patients and 21 healthy controls. The abundance change of serum miRNAs were also evaluated following surgical resection in 20 breast cancer patients. Receiver operating characteristic (ROC) curve analysis was performed to assess the sensitivity and specificity of miRNAs as diagnostic biomarkers. Results Serum levels of miR-21 and miR-155 was significantly higher, while miR-365 was significantly lower in breast cancer as compared with healthy controls. The serum levels of miR-21 and miR-155 significantly decreased following surgical resection. Additionally, the serum level of miR-155 at stages I and II was significantly higher compared to stage III. The serum miR-145 level was remarkably higher in progesterone receptor (PR)-positive patients than PR-negative. The positivity of miR-21, miR-155, and miR-365 was high compared to CA 153 and CEA in breast cancer. ROC curve analyses of a combination of miR-21, miR-155, and miR-365 yielded much higher area under curve and enhanced sensitivity and specificity in comparison to each miRNA alone. Conclusion The combination of serum miR-21/miR-155/miR-365 may potentially serve as a sensitive and specific biomarker that enables differentiation of breast cancer from healthy controls. PMID:28203552

  5. 强直性脊柱炎患者外周血中 miR-17、miR-181、miR-106、miR-30和miR-495表达的分析%miR-17,miR-181,miR-106,miR-30 and miR-495 level differences in peripheral blood of patients with ankylosing spondylitis

    Institute of Scientific and Technical Information of China (English)

    刘鑫; 魏军; 杨芝红; 周林林; 郑锡铭; 徐广贤

    2014-01-01

    目的:为了探讨microRNA在强直性脊柱炎患者发病过程中的作用,我们通过检测强直性脊柱炎患者外周血液中miR-17、miR-181、miR-106、miR-30和miR-495的表达情况,从而为研究microRNA在强直性脊柱炎发病机制中的调控作用和临床诊断价值提供新的思路。方法:收集强直性脊柱炎患者及正常人的外周血液,对外周血液中单个核细胞进行分离,并提取PBMC small RNA,用特异性茎环引物反转录成cDNA,并构建成熟的miR-17、miR-181、miR-106、miR-30和miR-495的T载体,制作标准曲线,利用stem-loop法通过Real-time PCR技术,检测miR-17、miR-181、miR-106、miR-30和miR-495的表达量。结果:本实验共收集临床样本92例,其中AS患者61例,正常人31例。通过Real-time PCR检测发现,与正常人相比,在强直性脊柱炎患者外周血中表达上调的有miR-106(P>0.05)与miR-30(P>0.05);表达下调的有miR-181(P<0.05)、 miR-495(P<0.05)和miR-17(P>0.05),其中miR-181和miR-495有统计学意义。结论:与正常人相比在强直性脊柱炎患者外周血中miR-181和miR-495的表达量存在差异,它们可以靶向调控TLR-4,HLA-B,DVL和GSK/3β等基因,这一发现可能为强直性脊柱炎发病机制的研究提供新的思路,成为临床诊断中新的标志物。%Objective:In order to investigate the role of microRNA in the pathogenesis of ankylosing spondylitis patients,we detected the peripheral blood of patients with ankylosing spondylitis in miR-17,miR-181,miR-106,miR-30 and miR-495 expression, thereby to study the role of microRNA regulation of clinical and diagnostic value in the pathogenesis of ankylosing spondylitis provide new ideas.Methods:Collection of patients with ankylosing spondylitis and normal peripheral blood,peripheral blood mononuclear cells were isolated and extracted PBMC small RNA,using primers specific stem-loop reverse transcribed into c

  6. miRNA signature identification of retinoblastoma and the correlations between differentially expressed miRNAs during retinoblastoma progression.

    Science.gov (United States)

    Yang, Yang; Mei, Qi

    2015-01-01

    Retinoblastoma (RB) is a common pediatric cancer. The study aimed to uncover the mechanisms of RB progression and identify novel therapeutic biomarkers. The miRNA expression profile GSE7072, which includes three RB samples and three healthy retina samples, was used. After data normalization using the preprocessCore package, differentially expressed miRNAs (DE-miRs) were selected by the limma package. The targets of the DE-miRs were predicted based on two databases, followed by construction of the miRNA-target network. Pathway enrichment analysis was conducted for the targets of the DE-miRNAs using DAVID. The CTD database was used to predict RB-related genes, followed by clustering analysis using the pvclust package. The correlation network of DE-miRs was established. MiRNA expression was validated in another data set, GSE41321. In total, 24 DE-miRs were identified whose targets were correlated with the cell cycle pathway. Among them, hsa-miR-373, hsa-miR-125b, and hsa-miR-181a were highlighted in the miRNA-target regulatory network; 14 DE-miRs, including hsa-miR-373, hsa-miR-125b, hsa-miR-18a, hsa-miR-25, hsa-miR-20a, and hsa-let-7 (a, b, c), were shown to distinguish RB from healthy tissue. In addition, hsa-miR-25, hsa-miR-18a, and hsa-miR-20a shared the common target BCL2L11; hsa-let-7b and hsa-miR-125b targeted the genes CDC25A, CDK6, and LIN28A. Expression of three miRNAs in GSE41321 was consistent with that in GSE7072. Several critical miRNAs were identified in RB progression. Hsa-miR-373 might regulate RB invasion and metastasis, hsa-miR-181a might involve in the CDKN1B-mediated cell cycle pathway, and hsa-miR-125b and hsa-let-7b might serve as tumor suppressors by coregulating CDK6, CDC25A, and LIN28A. The miRNAs hsa-miR-25, hsa-miR-18a, and hsa-miR-20a might exert their function by coregulating BCL2L1.

  7. miR-200 family and its mechanism%miR-200及其作用机制

    Institute of Scientific and Technical Information of China (English)

    苏娟; 张庆瑜

    2011-01-01

    miR-200是一簇与上皮间质转化(EMT)密切相关的微RNA(miRNA)家族,其家族成员包括miR-200a、miR-200b、miR-200c、miR-141及miR-429.miR-200是EMT的主要调节分子并可维持细胞的上皮表型,在EMT的发生和肿瘤的进展过程中发挥重要的作用.%MiR-200 family is a cluster of miRNAs closely linked to EMT. The miR-200 family contains miR-200a, miR-200b, miR-200c, miR-141 and miR-429. Many studies have shown that miR-200 family are key regulators of EMT and determine the epithelial phenotype. They are important to both EMT development and tumor progression. This paper reviews the advancement in the research of miR-200 family and its mechanism.

  8. MiR-221 and miR-130a regulate lung airway and vascular development.

    Directory of Open Access Journals (Sweden)

    Sana Mujahid

    Full Text Available Epithelial-mesenchymal interactions play a crucial role in branching morphogenesis, but very little is known about how endothelial cells contribute to this process. Here, we examined how anti-angiogenic miR-221 and pro-angiogenic miR-130a affect airway and vascular development in the fetal lungs. Lung-specific effects of miR-130a and miR-221 were studied in mouse E14 whole lungs cultured for 48 hours with anti-miRs or mimics to miR-130a and miR-221. Anti-miR 221 treated lungs had more distal branch generations with increased Hoxb5 and VEGFR2 around airways. Conversely, mimic 221 treated lungs had reduced airway branching, dilated airway tips and decreased Hoxb5 and VEGFR2 in mesenchyme. Anti-miR 130a treatment led to reduced airway branching with increased Hoxa5 and decreased VEGFR2 in the mesenchyme. Conversely, mimic 130a treated lungs had numerous finely arborized branches extending into central lung regions with diffusely localized Hoxa5 and increased VEGFR2 in the mesenchyme. Vascular morphology was analyzed by GSL-B4 (endothelial cell-specific lectin immunofluorescence. Observed changes in airway morphology following miR-221 inhibition and miR-130a enhancement were mirrored by changes in vascular plexus formation around the terminal airways. Mouse fetal lung endothelial cells (MFLM-91U were used to study microvascular cell behavior. Mimic 221 treatment resulted in reduced tube formation and cell migration, where as the reverse was observed with mimic 130a treatment. From these data, we conclude that miR-221 and miR-130a have opposing effects on airway and vascular morphogenesis of the developing lung.

  9. Identification of novel targets for miR-29a using miRNA proteomics.

    Directory of Open Access Journals (Sweden)

    Rhishikesh Bargaje

    Full Text Available MicroRNAs (miRNAs are short regulatory RNA molecules that interfere with the expression of target mRNA by binding to complementary sequences. Currently, the most common method for identification of targets of miRNAs is computational prediction based on free energy change calculations, target site accessibility and conservation. Such algorithms predict hundreds of targets for each miRNA, necessitating tedious experimentation to identify the few functional targets. Here we explore the utility of miRNA-proteomics as an approach to identifying functional miRNA targets. We used Stable Isotope Labeling by amino acids in cell culture (SILAC based proteomics to detect differences in protein expression induced by the over-expression of miR-34a and miR-29a. Over-expression of miR-29a, a miRNA expressed in the brain and in cells of the blood lineage, resulted in the differential expression of a set of proteins. Gene Ontology based classification showed that a significant sub-set of these targets, including Voltage Dependent Anion Channel 1 and 2 (VDAC1 and VDAC2 and ATP synthetase, were mitochondrial proteins involved in apoptosis. Using reporter assays, we established that miR-29a targets the 3' Untranslated Regions (3' UTR of VDAC1 and VDAC2. However, due to the limited number of proteins identified using this approach and the inability to differentiate between primary and secondary effects we conclude that miRNA-proteomics is of limited utility as a high-throughput alternative for sensitive and unbiased miRNA target identification. However, this approach was valuable for rapid assessment of the impact of the miRNAs on the cellular proteome and its biological role in apoptosis.

  10. Geology and Stratigraphy of the East and West Firing Areas Lawrence Livermore National Laboratory Site 300

    Energy Technology Data Exchange (ETDEWEB)

    Ehman, K D

    2006-05-10

    The purpose of this project is to gain a better understanding of the stratigraphy and geologic structure of the East and West Firing Areas, at Lawrence Livermore National Laboratory Site 300 (Figure 1). This analysis is designed to help better delineate hydrostratigraphic units (HSUs) in order to enhance the understanding of the fate and transport of contaminants in the subsurface. Specific objectives of the investigation include: (1) Evaluation of the stratigraphic relationships between the units that contain tritium in ground water that originates from Pit 7 and the Building 850 area in the vicinity of Doall Ravine; (2) The correlation of these units across the Elk Ravine Fault Zone; and (3) The correlation of these units between the Building 865, Pit 1, Pit 2, and Building 812 areas. These issues were raised by regulators at the Regional Water Quality Control Board in the review of the Pit 7 RI/FS (Taffet and others, 2005). The results of this investigation will assist Lawrence Livermore National Laboratory (LLNL) hydrogeologists to conduct work in a more focused and cost-effective manner. This document is submitted to fulfill contract obligations for subcontract B539658.

  11. Lawrence Livermore National Laboratory Emergency Response Capability 2009 Baseline Needs Assessment Performance Assessment

    Energy Technology Data Exchange (ETDEWEB)

    Sharry, J A

    2009-12-30

    This document was prepared by John A. Sharry, LLNL Fire Marshal and Division Leader for Fire Protection and was reviewed by Sandia/CA Fire Marshal, Martin Gresho. This document is the second of a two-part analysis of Emergency Response Capabilities of Lawrence Livermore National Laboratory. The first part, 2009 Baseline Needs Assessment Requirements Document established the minimum performance criteria necessary to meet mandatory requirements. This second part analyses the performance of Lawrence Livermore Laboratory Emergency Management Department to the contents of the Requirements Document. The document was prepared based on an extensive review of information contained in the 2004 BNA, a review of Emergency Planning Hazards Assessments, a review of building construction, occupancy, fire protection features, dispatch records, LLNL alarm system records, fire department training records, and fire department policies and procedures. On October 1, 2007, LLNL contracted with the Alameda County Fire Department to provide emergency response services. The level of service called for in that contract is the same level of service as was provided by the LLNL Fire Department prior to that date. This Compliance Assessment will evaluate fire department services beginning October 1, 2008 as provided by the Alameda County Fire Department.

  12. Trophic interactions in the St. Lawrence Estuary (Canada): Must the blue whale compete for krill?

    Science.gov (United States)

    Savenkoff, C.; Comtois, S.; Chabot, D.

    2013-09-01

    Inverse methodology was used to construct a mass-balance model of the Lower St. Lawrence Estuary (LSLE) for the 2008-2010 time period. Our first objective was to make an overall description of community structure, trophic interactions, and the effects of fishing and predation on the vertebrate and invertebrate communities of the ecosystem. A second objective was to identify other important predators of krill, and to assess if these compete with blue whales, listed as endangered under the Canadian Species at Risk Act in 2005 (northwest Atlantic population). The Estuary and the Gulf of St. Lawrence are summer feeding grounds for blue whales and other marine mammals. Blue whales eat only euphausiids (krill) and require dense concentrations of prey to meet their energy requirements, which makes them particularly vulnerable to changes in prey availability. In the LSLE, many species from secondary producers (hyperiid amphipods, other macrozooplankton) to top predators (fish, birds, and marine mammals) consumed euphausiids. Consequently, krill predators were found at all consumer trophic levels. However, our results showed that only about 35% of the estimated euphausiid production was consumed by all predator species combined. Euphausiid did not seem to be a restricted resource in the LSLE ecosystem, at least during the study period. The blue whale did not appear to have to compete for krill in the LSLE.

  13. Lawrence Livermore National Laboratory Experimental Test Site (Site 300) Potable Water System Operations Plan

    Energy Technology Data Exchange (ETDEWEB)

    Ocampo, Ruben P. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Bellah, Wendy [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2016-03-04

    The existing Lawrence Livermore National Laboratory (LLNL) Site 300 drinking water system operation schematic is shown in Figures 1 and 2 below. The sources of water are from two Site 300 wells (Well #18 and Well #20) and San Francisco Public Utilities Commission (SFPUC) Hetch-Hetchy water through the Thomas shaft pumping station. Currently, Well #20 with 300 gallons per minute (gpm) pump capacity is the primary source of well water used during the months of September through July, while Well #18 with 225 gpm pump capacity is the source of well water for the month of August. The well water is chlorinated using sodium hypochlorite to provide required residual chlorine throughout Site 300. Well water chlorination is covered in the Lawrence Livermore National Laboratory Experimental Test Site (Site 300) Chlorination Plan (“the Chlorination Plan”; LLNL-TR-642903; current version dated August 2013). The third source of water is the SFPUC Hetch-Hetchy Water System through the Thomas shaft facility with a 150 gpm pump capacity. At the Thomas shaft station the pumped water is treated through SFPUC-owned and operated ultraviolet (UV) reactor disinfection units on its way to Site 300. The Thomas Shaft Hetch- Hetchy water line is connected to the Site 300 water system through the line common to Well pumps #18 and #20 at valve box #1.

  14. Catalog of Research Abstracts, 1993: Partnership opportunities at Lawrence Berkeley Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    1993-09-01

    The 1993 edition of Lawrence Berkeley Laboratory`s Catalog of Research Abstracts is a comprehensive listing of ongoing research projects in LBL`s ten research divisions. Lawrence Berkeley Laboratory (LBL) is a major multi-program national laboratory managed by the University of California for the US Department of Energy (DOE). LBL has more than 3000 employees, including over 1000 scientists and engineers. With an annual budget of approximately $250 million, LBL conducts a wide range of research activities, many that address the long-term needs of American industry and have the potential for a positive impact on US competitiveness. LBL actively seeks to share its expertise with the private sector to increase US competitiveness in world markets. LBL has transferable expertise in conservation and renewable energy, environmental remediation, materials sciences, computing sciences, and biotechnology, which includes fundamental genetic research and nuclear medicine. This catalog gives an excellent overview of LBL`s expertise, and is a good resource for those seeking partnerships with national laboratories. Such partnerships allow private enterprise access to the exceptional scientific and engineering capabilities of the federal laboratory systems. Such arrangements also leverage the research and development resources of the private partner. Most importantly, they are a means of accessing the cutting-edge technologies and innovations being discovered every day in our federal laboratories.

  15. MiBoard: Multiplayer Interactive Board Game

    CERN Document Server

    Dempsey, Kyle B; Jackson, G Tanner; Boonthum, Chutima; Levinstein, Irwin B; McNamara, Danielle S

    2010-01-01

    Serious games have recently emerged as an avenue for curriculum delivery. Serious games incorporate motivation and entertainment while providing pointed curriculum for the user. This paper presents a serious game, called MiBoard, currently being developed from the iSTART Intelligent Tutoring System. MiBoard incorporates a multiplayer interaction that iSTART was previously unable to provide. This multiplayer interaction produces a wide variation across game trials, while also increasing the repeat playability for users. This paper presents a demonstration of the MiBoard system and the expectations for its application.

  16. Foreword: can Glucksberg survive Lawrence? Another look at the end of life and personal autonomy.

    Science.gov (United States)

    Kamisar, Yale

    2008-06-01

    In Washington v. Glucksberg, the Court declined to find a right to physician-assisted suicide ("PAS") in the Constitution. Not a single Justice dissented. One would expect such a ruling to be quite secure. But Lawrence v. Texas, holding that a state cannot make consensual homosexual conduct a crime, is not easy to reconcile with Glucksberg. Lawrence certainly takes a much more expansive view of substantive due process than did Glucksberg. It is conceivable that the five Justices who made up the Lawrence majority--all of whom still sit on the Court--might overrule Glucksberg. For various reasons, however, this seems improbable. Unlike the situation with respect to the pre-Lawrence era, Glucksberg does not stigmatize any politically vulnerable group. When there is no democratic defect in the political process, there is much to be said for courts deferring to reasonable legislative judgments. Moreover, unlike the developments preceding Lawrence, there has been no emerging awareness of a right or liberty to enlist the assistance of a physician in committing suicide. No state supreme court has found a right to PAS in its own state constitution. Nor, in the decade since Glucksberg, has any state legislature legalized PAS. And attempts have been made to do so in some twenty states. In addition, various considerations might cause a court to balk at constitutionalizing PAS for the terminally ill. Such a right is not easily cabined. If personal autonomy extends to the time and manner of one's death, why doesn't it also apply whenever a competent person believes that death is better than continued life? Once the right to PAS is grounded on self-determination or personal autonomy in controlling ones own life and death, it no longer seems plausible to limit it to the terminally ill. Why should people who have to endure pain, suffering, or indignity for a much longer time than the terminally ill (often defined as those with six months or less to live) be denied this right? The

  17. Inference of Gene Regulation via miRNAs During ES Cell Differentiation Using MiRaGE Method

    Directory of Open Access Journals (Sweden)

    Jun Yasuda

    2011-12-01

    Full Text Available MicroRNA (miRNA is a critical regulator of cell growth, differentiation, and development. To identify important miRNAs in a biological process, many bioinformatical tools have been developed. We have developed MiRaGE (MiRNA Ranking by Gene Expression method to infer the regulation of gene expression by miRNAs from changes of gene expression profiles. The method does not require precedent array normalization. We applied the method to elucidate possibly important miRNAs during embryonic stem (ES cell differentiation to neuronal cells and we infer that certain miRNAs, including miR-200 family, miR-429, miR-302 family, and miR-17-92 cluster members may be important to the maintenance of undifferentiated status in ES cells.

  18. Using miRNA-Analyzer for the Analysis of miRNA Data

    Science.gov (United States)

    Guzzi, Pietro Hiram; Tradigo, Giuseppe; Veltri, Pierangelo

    2016-01-01

    MicroRNAs (miRNAs) are small biological molecules that play an important role during the mechanisms of protein formation. Recent findings have demonstrated that they act as both positive and negative regulators of protein formation. Thus, the investigation of miRNAs, i.e., the determination of their level of expression, has developed a huge interest in the scientific community. One of the leading technologies for extracting miRNA data from biological samples is the miRNA Affymetrix platform. It provides the quantification of the level of expression of the miRNA in a sample, thus enabling the accumulation of data and allowing the determination of relationships among miRNA, genes, and diseases. Unfortunately, there is a lack of a comprehensive platform able to provide all the functions needed for the extraction of information from miRNA data. We here present miRNA-Analyzer, a complete software tool providing primary functionalities for miRNA data analysis. The current version of miRNA-Analyzer wraps the Affymetrix QCTool for the preprocessing of binary data files, and then provides feature selection (the filtering by species and by the associated p-value of preprocessed files). Finally, preprocessed and filtered data are analyzed by the Multiple Experiment Viewer (T-MEV) and Short Time Series Expression Miner (STEM) tools, which are also wrapped into miRNA-Analyzer, thus providing a unique environment for miRNA data analysis. The tool offers a plug-in interface so it is easily extensible by adding other algorithms as plug-ins. Users may download the tool freely for academic use at https://sites.google.com/site/mirnaanalyserproject/d. PMID:27983673

  19. MicroRNAs, macrocontrol : Regulation of miRNA processing

    NARCIS (Netherlands)

    Slezak-Prochazka, Izabella; Durmus, Selvi; Kroesen, Bart-Jan; van den Berg, Anke

    2010-01-01

    MicroRNAs (miRNAs) are a set of small, non-protein-coding RNAs that regulate gene expression at the post-transcriptional level. Maturation of miRNAs comprises several regulated steps resulting in similar to 22-nucleotide single-stranded mature miRNAs. Regulation of miRNA expression can occur both at

  20. 78 FR 15796 - Michigan Disaster #MI-00038.

    Science.gov (United States)

    2013-03-12

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Michigan Disaster MI-00038. AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY... adversely affected by the disaster: Primary Counties: Mecosta. Contiguous Counties: Michigan:...

  1. Lo que mi abuelo me cuenta

    OpenAIRE

    Benavides Muhamad, Amal; Pontificia Universidad Javeriana Cali

    2014-01-01

    En el estado de Palestina, ubicado en oriente medio, hace más de 57 años en Kofor malek –un pequeño pueblo– vivía una familia muy numerosa. Era la familia de mi abuelo que estaba conformada por once personas incluyendo a sus padres. Como la describe el, su familia la integraban: “Mi madre, una mujer muy cariñosa, excelente cocinera y muy dedicada a su hogar; la madre típica de ese entonces que casi nunca salía del hogar. Su vida giraba en torno a mi padre y a nosotros, sus hijos. Mi padre un ...

  2. The NuMI Neutrino Beam

    CERN Document Server

    Adamson, P; Andrews, M; Andrews, R; Anghel, I; Augustine, D; Aurisano, A; Avvakumov, S; Ayres, D S; Baller, B; Barish, B; Barr, G; Barrett, W L; Bernstein, R H; Biggs, J; Bishai, M; Blake, A; Bocean, V; Bock, G J; Boehnlein, D J; Bogert, D; Bourkland, K; Cao, S V; Castromonte, C M; Childress, S; Choudhary, B C; Coelho, J A B; Cobb, J H; Corwin, L; Crane, D; Cravens, J P; Cronin-Hennessy, D; Ducar, R J; de Jong, J K; Devan, A V; Devenish, N E; Diwan, M V; Erwin, A R; Escobar, C O; Evans, J J; Falk, E; Feldman, G J; Fields, T H; Ford, R; Frohne, M V; Gallagher, H R; Garkusha, V; Gomes, R A; Goodman, M C; Gouffon, P; Graf, N; Gran, R; Grossman, N; Grzelak, K; Habig, A; Hahn, S R; Harding, D; Harris, D; Harris, P G; Hartnell, J; Hatcher, R; Hays, S; Heller, K; Holin, A; Huang, J; Hylen, J; Ibrahim, A; Indurthy, D; Irwin, G M; Isvan, Z; Jaffe, D E; James, C; Jensen, D; Johnstone, J; Kafka, T; Kasahara, S M S; Koizumi, G; Kopp, S; Kordosky, M; Kreymer, A; Lang, K; Laughton, C; Lefeuvre, G; Ling, J; Litchfield, P J; Loiacono, L; Lucas, P; Mann, W A; Marchionni, A; Marshak, M L; Mayer, N; McGivern, C; Medeiros, M M; Mehdiyev, R; Meier, J R; Messier, M D; Michael, D G; Milburn, R H; Miller, J L; Miller, W H; Mishra, S R; Sher, S Moed; Moore, C D; Morfin, J; Mualem, L; Mufson, S; Murgia, S; Murtagh, M; Musser, J; Naples, D; Nelson, J K; Newman, H B; Nichol, R J; Nowak, J A; Connor, J O; Oliver, W P; Olsen, M; Orchanian, M; Osprey, S; Pahlka, R B; Paley, J; Para, A; Patterson, R B; Patzak, T; Pavlovic, Z; Pawloski, G; Perch, A; Peterson, E A; Petyt, D A; Pfutzner, M; Phan-Budd, S; Plunkett, R K; Poonthottathil, N; Prieto, P; Pushka, D; Qiu, X; Radovic, A; Rameika, R A; Ratchford, J; Rebel, B; Reilly, R; Rosenfeld, C; Rubin, H A; Ruddick, K; Sanchez, M C; Saoulidou, N; Sauer, L; Schneps, J; Schoo, D; Schreckenberger, A; Schreiner, P; Shanahan, P; Sharma, R; Smart, W; Smith, C; Sousa, A; Stefanik, A; Tagg, N; Talaga, R L; Tassotto, G; Thomas, J; Thompson, J; Thomson, M A; Tian, X; Timmons, A; Tinsley, D; Tognini, S C; Toner, R; Torretta, D; Trostin, I; Tzanakos, G; Urheim, J; Vahle, P; Vaziri, K; Villegas, E; Viren, B; Vogel, G; Webber, R C; Weber, A; Webb, R C; Wehmann, A; White, C; Whitehead, L; Whitehead, L H; Wojcicki, S G; Wong-Squires, M L; Yang, T; Yumiceva, F X; Zarucheisky, V; Zwaska, R

    2015-01-01

    This paper describes the hardware and operations of the Neutrinos at the Main Injector (NuMI) beam at Fermilab. It elaborates on the design considerations for the beam as a whole and for individual elements. The most important design details of individual components are described. Beam monitoring systems and procedures, including the tuning and alignment of the beam and NuMI long-term performance, are also discussed.

  3. The NuMI neutrino beam

    Energy Technology Data Exchange (ETDEWEB)

    Adamson, P.; Anderson, K.; Andrews, M.; Andrews, R. [Fermi National Accelerator Laboratory, Batavia, IL 60510 (United States); Anghel, I. [Department of Physics and Astronomy, Iowa State University, Ames, IA 50011 (United States); Argonne National Laboratory, Argonne, IL 60439 (United States); Augustine, D. [Fermi National Accelerator Laboratory, Batavia, IL 60510 (United States); Aurisano, A. [Department of Physics, University of Cincinnati, Cincinnati, OH 45221 (United States); Avvakumov, S. [Department of Physics, Stanford University, Stanford, CA 94305 (United States); Ayres, D.S. [Argonne National Laboratory, Argonne, IL 60439 (United States); Baller, B. [Fermi National Accelerator Laboratory, Batavia, IL 60510 (United States); Barish, B. [Lauritsen Laboratory, California Institute of Technology, Pasadena, CA 91125 (United States); Barr, G. [Subdepartment of Particle Physics, University of Oxford, Oxford OX1 3RH (United Kingdom); Barrett, W.L. [Physics Department, Western Washington University, Bellingham, WA 98225 (United States); Bernstein, R.H.; Biggs, J. [Fermi National Accelerator Laboratory, Batavia, IL 60510 (United States); Bishai, M. [Brookhaven National Laboratory, Upton, NY 11973 (United States); Blake, A. [Cavendish Laboratory, University of Cambridge, Madingley Road, Cambridge CB3 0HE (United Kingdom); Bocean, V.; Bock, G.J.; Boehnlein, D.J. [Fermi National Accelerator Laboratory, Batavia, IL 60510 (United States); and others

    2016-01-11

    This paper describes the hardware and operations of the Neutrinos at the Main Injector (NuMI) beam at Fermilab. It elaborates on the design considerations for the beam as a whole and for individual elements. The most important design details of individual components are described. Beam monitoring systems and procedures, including the tuning and alignment of the beam and NuMI long-term performance, are also discussed.

  4. 劳伦斯小说中的生命哲学书写%Life philosophy in Lawrence's novel

    Institute of Scientific and Technical Information of China (English)

    褚金月

    2015-01-01

    Lawrence became famous after his death, he was influenced by Nietzsche's philosophy of life, the novel focuses on the tragedy, sex and rebellion of religion and theology, these aspects have important value on research Lawrence novels.%劳伦斯死后才声名鹊起,其生前深受尼采生命哲学影响,小说专注于对悲剧、性爱和对宗教神学的反叛,从这些方面出发,对研究劳伦斯小说有着重要价值和作用.

  5. Characterization of St. Lawrence blue whale vocalizations and their correlation with field observations

    Science.gov (United States)

    Berchok, Catherine L.

    During four field seasons from 1998--2001, 115 hours of acoustic recordings were made in the presence of the well-studied St. Lawrence population of blue whales. The primary field site for this study was the estuary region of the St. Lawrence River (Quebec, Canada) with most recordings made between mid-August and late October. Effort was concentrated in the daylight hours, although occasionally extending past nightfall. An inexpensive and portable recording system was built that was easy to deploy and provided quality recordings in a variety of sea conditions. It consisted of a calibrated omni-directional hydrophone with a flat (+/-3dB) response from 5Hz to 800Hz; and a surface isolation buoy to minimize the vertical movement of the sensor. During the recording sessions detailed field notes were taken on all blue whales within sight, with individual identities confirmed through photo-identification work between sessions. Notes were also taken on all other species sighted during the recording sessions. Characterization of the more than one-thousand blue whale calls detected during this study revealed that the St. Lawrence repertoire is much more extensive than previously reported. Three infrasonic (<20Hz) and four audible range (30--200Hz) call types were detected in this study, with much time/frequency variation seen within each type. The infrasonic calls were long (5--30s) in duration and arranged into regularly patterned series. These calls were similar in call characteristics and spacing to those detected in the North Atlantic, but had much shorter and more variable patterned series. The audible call types were much shorter (1--4s), and occurred singly or in irregularly spaced clusters, although a special patterning was seen that contained both regular and irregular spaced components. Comparison of the daily, seasonal, and spatial distributions of calling behavior with those of several biological parameters revealed interesting differences between the three call

  6. A conserved gene structure and expression regulation of miR-433 and miR-127 in mammals.

    Directory of Open Access Journals (Sweden)

    Guisheng Song

    Full Text Available MicroRNAs play essential roles in many cellular processes. However, limited information is available regarding the gene structure and transcriptional regulation of miRNAs. We explored the gene cluster encoding miR-433/127 in mammalian species using bioinformatics and in vitro "gene" expression approaches. Multiple sequence alignments (MSA showed that the precursors of miR-433 and of miR-127 exhibited 95% and 100% similarity, respectively, in human, chimpanzee, horse, dog, monkey, rat, cow, and mouse. MSA of the promoter sequences of miR-433 and of miR-127 revealed lower sequence similarity among these mammalian species. However, the distance between miR-433 and miR-127 was strikingly similar, which was between 986 and 1007 bp and the position of transcription factor (TF binding motifs, including estrogen related receptor response element (ERRE, was well conserved. Transient transfection assays showed that promoters of miR-433 and of miR-127 from human, rat, and dog were activated by estrogen related receptor gamma (ERRgamma and inhibited by small heterodimer partner (SHP. ChIP assays confirmed the physical association of ERRgamma with the endogenous promoters of miR-433 and miR-127. In vitro over-expression of the human, rat, or dog miR-433/127 loci in cells, using an expression vector containing miR-433/127 and their promoter regions, markedly induced a differential expression of both primary and mature miR-433 and miR-127, indicating that miR-433 and miR-127 were possessed from their independent promoters. Our studies for the first time demonstrate a conserved gene structure and transcriptional regulation of miR-433 and miR-127 in mammals. The data suggest that the miR-433/127 loci may have evolved from a common gene of origin.

  7. Coordinated epigenetic repression of the miR-200 family and miR-205 in invasive bladder cancer

    DEFF Research Database (Denmark)

    Wiklund, Erik D; Bramsen, Jesper B; Hulf, Toby

    2011-01-01

    MicroRNAs (miRNA) are small noncoding RNAs commonly deregulated in cancer. The miR-200 family (miR-200a, -200b, -200c, -141 and -429) and miR-205 are frequently silenced in advanced cancer and have been implicated in epithelial to mesenchymal transition (EMT) and tumor invasion by targeting the t...

  8. Coordinated epigenetic repression of the miR-200 family and miR-205 in invasive bladder cancer

    DEFF Research Database (Denmark)

    Wiklund, Erik D; Bramsen, Jesper B; Hulf, Toby

    2011-01-01

    MicroRNAs (miRNA) are small noncoding RNAs commonly deregulated in cancer. The miR-200 family (miR-200a, -200b, -200c, -141 and -429) and miR-205 are frequently silenced in advanced cancer and have been implicated in epithelial to mesenchymal transition (EMT) and tumor invasion by targeting...

  9. Role of miR-1 and miR-133a in myocardial ischemic postconditioning

    Directory of Open Access Journals (Sweden)

    Xie Bing

    2011-03-01

    Full Text Available Abstract Background Ischemic postconditioning (IPost has aroused much attention since 2003 when it was firstly reported. The role of microRNAs (miRNAs or miRs in IPost has rarely been reported. The present study was undertaken to investigate whether miRNAs were involved in the protective effect of IPost against myocardial ischemia-reperfusion (IR injury and the probable mechanisms involved. Methods Thirty SD rats weighing 250-300 g were equally randomized to three groups: Control group, where the rats were treated with thoracotomy only; IR group, where the rats were treated with ischemia for 60 min and reperfusion for 180 min; and IPost group, where the rats were treated with 3 cycles of transient IR just before reperfusion. The extent of myocardial infarction, LDH and CK activities were measured immediately after treatment. Myocardial apoptosis was detected by TUNEL assay. The myocardial tissue was collected after IR or IPost stimulation to evaluate the miRNAs expression level by miRNA-microarray and quantitative real-time RT-PCR. Real-time PCR was conducted to identify changes in mRNA expression of apoptosis-related genes such as Bcl-2, Bax and Caspase-9 (CASP9, and Western blot was used to compare the protein expression level of CASP9 in the three groups. The miRNA mimics and anti-miRNA oligonucleotides (AMO were transferred into the cultured neonatal cardiomyocytes and myocardium before they were treated with IR. The effect of miRNAs on apoptosis was determined by flow cytometry and TUNEL assay. CASP9, as one of the candidate target of miR-133a, was compared during IR after the miR-133a mimic or AMO-133a was transferred into the myocardium. Results IPost reduced the IR-induced infarct size of the left ventricle, and decreased CK and LDH levels. TUNEL assay showed that myocardial apoptosis was attenuated by IPost compared with IR. MiRNA-microarray and RT-PCR showed that myocardial-specific miR-1 and miR-133a were down-regulated by IR, and up

  10. MiRNATIP: a SOM-based miRNA-target interactions predictor.

    Science.gov (United States)

    Fiannaca, Antonino; Rosa, Massimo La; Paglia, Laura La; Rizzo, Riccardo; Urso, Alfonso

    2016-09-22

    MicroRNAs (miRNAs) are small non-coding RNA sequences with regulatory functions to post-transcriptional level for several biological processes, such as cell disease progression and metastasis. MiRNAs interact with target messenger RNA (mRNA) genes by base pairing. Experimental identification of miRNA target is one of the major challenges in cancer biology because miRNAs can act as tumour suppressors or oncogenes by targeting different type of targets. The use of machine learning methods for the prediction of the target genes is considered a valid support to investigate miRNA functions and to guide related wet-lab experiments. In this paper we propose the miRNA Target Interaction Predictor (miRNATIP) algorithm, a Self-Organizing Map (SOM) based method for the miRNA target prediction. SOM is trained with the seed region of the miRNA sequences and then the mRNA sequences are projected into the SOM lattice in order to find putative interactions with miRNAs. These interactions will be filtered considering the remaining part of the miRNA sequences and estimating the free-energy necessary for duplex stability. We tested the proposed method by predicting the miRNA target interactions of both the Homo sapiens and the Caenorhbditis elegans species; then, taking into account validated target (positive) and non-target (negative) interactions, we compared our results with other target predictors, namely miRanda, PITA, PicTar, mirSOM, TargetScan and DIANA-microT, in terms of the most used statistical measures. We demonstrate that our method produces the greatest number of predictions with respect to the other ones, exhibiting good results for both species, reaching the for example the highest percentage of sensitivity of 31 and 30.5 %, respectively for Homo sapiens and for C. elegans. All the predicted interaction are freely available at the following url: http://tblab.pa.icar.cnr.it/public/miRNATIP/ . Results state miRNATIP outperforms or is comparable to the other six state

  11. Analyzing miRNA co-expression networks to explore TF-miRNA regulation

    Directory of Open Access Journals (Sweden)

    Bhattacharyya Malay

    2009-05-01

    Full Text Available Abstract Background Current microRNA (miRNA research in progress has engendered rapid accumulation of expression data evolving from microarray experiments. Such experiments are generally performed over different tissues belonging to a specific species of metazoan. For disease diagnosis, microarray probes are also prepared with tissues taken from similar organs of different candidates of an organism. Expression data of miRNAs are frequently mapped to co-expression networks to study the functions of miRNAs, their regulation on genes and to explore the complex regulatory network that might exist between Transcription Factors (TFs, genes and miRNAs. These directions of research relating miRNAs are still not fully explored, and therefore, construction of reliable and compatible methods for mining miRNA co-expression networks has become an emerging area. This paper introduces a novel method for mining the miRNA co-expression networks in order to obtain co-expressed miRNAs under the hypothesis that these might be regulated by common TFs. Results Three co-expression networks, configured from one patient-specific, one tissue-specific and a stem cell-based miRNA expression data, are studied for analyzing the proposed methodology. A novel compactness measure is introduced. The results establish the statistical significance of the sets of miRNAs evolved and the efficacy of the self-pruning phase employed by the proposed method. All these datasets yield similar network patterns and produce coherent groups of miRNAs. The existence of common TFs, regulating these groups of miRNAs, is empirically tested. The results found are very promising. A novel visual validation method is also proposed that reflects the homogeneity as well as statistical properties of the grouped miRNAs. This visual validation method provides a promising and statistically significant graphical tool for expression analysis. Conclusion A heuristic mining methodology that resembles a

  12. Computing environment for the ASSIST data warehouse at Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Shuk, K.

    1995-11-01

    The current computing environment for the ASSIST data warehouse at Lawrence Livermore National Laboratory is that of a central server that is accessed by a terminal or terminal emulator. The initiative to move to a client/server environment is strong, backed by desktop machines becoming more and more powerful. The desktop machines can now take on parts of tasks once run entirely on the central server, making the whole environment computationally more efficient as a result. Services are tasks that are repeated throughout the environment such that it makes sense to share them; tasks such as email, user authentication and file transfer are services. The new client/;server environment needs to determine which services must be included in the environment for basic functionality. These services then unify the computing environment, not only for the forthcoming ASSIST+, but for Administrative Information Systems as a whole, joining various server platforms with heterogeneous desktop computing platforms.

  13. Computer-aided mapping of stream channels beneath the Lawrence Livermore National Laboratory Super Fund Site

    Energy Technology Data Exchange (ETDEWEB)

    Sick, M. [Lawrence Livermore National Lab., CA (United States)

    1994-12-01

    The Lawrence Livermore National Laboratory (LLNL) site rests upon 300-400 feet of highly heterogeneous braided stream sediments which have been contaminated by a plume of Volatile Organic Compounds (VOCs). The stream channels are filled with highly permeable coarse grained materials that provide quick avenues for contaminant transport. The plume of VOCs has migrated off site in the TFA area, making it the area of greatest concern. I mapped the paleo-stream channels in the TFA area using SLICE an LLNL Auto-CADD routine. SLICE constructed 2D cross sections and sub-horizontal views of chemical, geophysical, and lithologic data sets. I interpreted these 2D views as a braided stream environment, delineating the edges of stream channels. The interpretations were extracted from Auto-CADD and placed into Earth Vision`s 3D modeling and viewing routines. Several 3D correlations have been generated, but no model has yet been chosen as a best fit.

  14. Safety analysis report for the Heavy-Element Facility (Building 251), Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Kvam, D.J.

    1982-10-11

    A comprehensive safety analysis was performed on the Lawrence Livermore National Laboratory's Heavy Element Facility, Building 251. The purpose of the analysis was to evaluate the building and its operations in order to inform LLNL and the Department of Energy of the risks they assume at Building 251. This was done by examining all of the energy sources and matching them with the physical and administrative barriers that control, prevent, or mitigate their hazards. Risk was evaluated for each source under both normal and catastrophic circumstances such as fire, flood, high wind, lighting, earthquake, and criticality. No significant safety deficiencies were found; it is concluded that the operation of the facility presents no unacceptable risk.

  15. Hazardous waste site assessment: Inactive landfill, Site 300, Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    1985-01-01

    This report presents the results of an investigation of an inactive landfill (Pit 6) at Lawrence Livermore National Laboratory's (LLNL) Site 300. The primary objectives were to: collect and review background information pertaining to past waste disposal practices and previous environmental characterization studies; conduct a geophysical survey of the landfill area to locate the buried wastes; conduct a hydrogeologic investigation to provide additional data on the rate and direction of groundwater flow, the extent of any groundwater contamination, and to investigate the connection, if any, of the shallow groundwater beneath the landfill with the local drinking water supply; conduct a risk assessment to identify the degree of threat posed by the landfill to the public health and environment; compile a preliminary list of feasible long-term remedial action alternatives for the landfill; and develop a list of recommendations for any interim measures necessary at the landfill should the long-term remedial action plan be needed.

  16. Fifty Years of Progress, 1937-1987 [Lawrence Berkeley Laboratory (LBL, LBNL)

    Science.gov (United States)

    Budinger, T. F. (ed.)

    1987-01-01

    This booklet was prepared for the 50th anniversary of medical and biological research at the Donner Laboratory and the Lawrence Berkeley Laboratory of the University of California. The intent is to present historical facts and to highlight important facets of fifty years of accomplishments in medical and biological sciences. A list of selected scientific publications from 1937 to 1960 is included to demonstrate the character and lasting importance of early pioneering work. The organizational concept is to show the research themes starting with the history, then discoveries of medically important radionuclides, then the use of accelerated charged particles in therapy, next human physiology studies then sequentially studies of biology from tissues to macromolecules; and finally studies of the genetic code.

  17. Federal Facility Compliance Act: Conceptual Site Treatment Plan for Lawrence Livermore National Laboratory, Livermore, California

    Energy Technology Data Exchange (ETDEWEB)

    1993-10-01

    The Department of Energy (DOE) is required by section 3021(b) of the Resource Conservation and Recovery Act (RCRA), as amended by the Federal Facility Compliance Act (the Act), to prepare plans describing the development of treatment capacities and technologies for treating mixed waste. The Act requires site treatment plans (STPs or plans) to be developed for each site at which DOE generates or stores mixed waste and submitted to the State or EPA for approval, approval with modification, or disapproval. The Lawrence Livermore National Laboratory (LLNL) Conceptual Site Treatment Plan (CSTP) is the preliminary version of the plan required by the Act and is being provided to California, the US Environmental Protection Agency (EPA), and others for review. A list of the other DOE sites preparing CSTPs is included in Appendix 1.1 of this document. Please note that Appendix 1.1 appears as Appendix A, pages A-1 and A-2 in this document.

  18. Remedial investigation of the High-Explosives (HE) Process Area, Lawrence Livermore National Laboratory Site 300

    Energy Technology Data Exchange (ETDEWEB)

    Crow, N.B.; Lamarre, A.L.

    1990-08-01

    This report presents the results of a Remedial Investigation (RI) to define the extent of high explosives (HE) compounds and volatile organic compounds (VOCs) found in the soil, rocks, and ground water of the HE Process Area of Lawrence Livermore National Laboratory's (LLNL) Site 300 Facility. The report evaluates potential public health environmental risks associated with these compounds. Hydrogeologic information available before February 15, 1990, is included; however, chemical analyses and water-level data are reported through March 1990. This report is intended to assist the California Regional Water Quality Control Board (RWQCB)--Central Valley Region and the US Environmental Protection Agency (EPA) in evaluating the extent of environmental contamination of the LLNL HE Process Area and ultimately in designing remedial actions. 90 refs., 20 figs., 7 tabs.

  19. Lawrence Berkeley Laboratory, Berkeley, California solar energy system performance evaluation, July 1980-June 1981

    Energy Technology Data Exchange (ETDEWEB)

    Wetzel, P.E.

    1981-01-01

    The Lawrence Berkeley Laboratory site is an office building in California with an active solar energy system designed to supply from 23 to 33% of the space heating load and part of the hot water load. The solar heating system is equipped with 1428 square feet of flat-plate collectors, a 2000-gallon water storage tank, and two gas-fired boilers to supply auxiliary heat for both space heating and domestic hot water. Poor performance is reported, with the solar fraction being only 4%. Also given are the solar savings ratio, conventional fuel savings, system performance factor, and the coefficient of performance. The performance data are given for the collector, storage, solar water heating and solar space heating subsystems as well as the total system. Typical system operation and solar energy utilization are briefly described. The system design, performance evaluation techniques, weather data, and sensor technology are presented. (LEW)

  20. Lawrence Livermore National Laboratory low-level waste systems performance assessment

    Energy Technology Data Exchange (ETDEWEB)

    1990-11-01

    This Low-Level Radioactive Waste (LLW) Systems Performance Assessment (PA) presents a systematic analysis of the potential risks posed by the Lawrence Livermore National Laboratory (LLNL) waste management system. Potential risks to the public and environment are compared to established performance objectives as required by DOE Order 5820.2A. The report determines the associated maximum individual committed effective dose equivalent (CEDE) to a member of the public from LLW and mixed waste. A maximum annual CEDE of 0.01 mrem could result from routine radioactive liquid effluents. A maximum annual CEDE of 0.003 mrem could result from routine radioactive gaseous effluents. No other pathways for radiation exposure of the public indicated detectable levels of exposure. The dose rate, monitoring, and waste acceptance performance objectives were found to be adequately addressed by the LLNL Program. 88 refs., 3 figs., 17 tabs.

  1. Environmental monitoring at the Lawrence Livermore National Laboratory: 1986 annual report

    Energy Technology Data Exchange (ETDEWEB)

    Holland, R.C.; Buddemeier, R.W.; Brekke, D.D.

    1987-04-01

    This report documents the results of the environmental monitoring program at the Lawrence Livermore National Laboratory (LLNL) for 1986. To evaluate the effect of LLNL operations on the local environment, measurements of direct radiation and a variety of radionuclides and chemical pollutants in ambient air, soil, surface water, groundwater, vegetation, milk, foodstuff, and sewage effluents were made at both the Livermore site and nearby Site 300. This report was prepared to meet the requirements of DOE Order 5484.1. Evaluations are made of LLNL's compliance with all applicable guides, standards, and limits for radiological and nonradiological releases to the environment. The data indicate that no releases in excess of the applicable standards were made during 1986, and that LLNL operations had no adverse environmental impact.

  2. Overview of Tabletop X-ray Laser Development at the Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Dunn, J; Shlyaptsev, V; Nilsen, J; Smith, R; Keenan, R; Moon, S; Filevich, J; Rocca, J; Nelson, A; Hunter, J; Marconi, M; Li, Y; Osterheld, A; Shepherd, R; Fiedorowicz, H; Bartnik, A; Faenov, A Y; Pikuz, T; Zeitoun, P; Hubert, S; Jacquemot, S; Fajardo, M

    2006-11-03

    It is almost a decade since the first tabletop x-ray laser experiments were implemented at the Lawrence Livermore National Laboratory (LLNL). The decision to pursue the picosecond-driven schemes at LLNL was largely based around the early demonstration of the tabletop Ne-like Ti x-ray laser at the Max Born Institute (MBI) as well as the established robustness of collisional excitation schemes. These picosecond x-ray lasers have been a strong growth area for x-ray laser research. Rapid progress in source development and characterization has achieved ultrahigh peak brightness rivaling the previous activities on the larger facilities. Various picosecond soft-x-ray based applications have benefited from the increased repetition rates. We will describe the activities at LLNL in this area.

  3. Environmental monitoring at the Lawrence Livermore National Laboratory: Annual report, 1987

    Energy Technology Data Exchange (ETDEWEB)

    Holland, R.C.; Brekke, D.D.

    1988-04-01

    This report documents the results of the Environmental Monitoring Program at the Lawrence Livermore Laboratory (LLNL) for 1987. To evaluate the effect of LLNL operations on the local environment, measurements were made of direct radiation and a variety of radionuclides and chemical pollutants in ambient air, soil, sewage effluents, surface water, groundwater, vegetation, foodstuff, and milk at both the Livermore site and nearby Site 300. Evaluations were made of LLNL's compliance with the applicable guides, standards, and limits for radiological and nonradiological releases to the environment. The data indicates that the only releases in excess of applicable standards were four releases to the sanitary sewer. LLNL operations had no adverse impact on the environment during 1987. 65 refs., 24 figs.

  4. Physical growth of St. Lawrence Island Eskimos: body size, proportion, and composition.

    Science.gov (United States)

    Johnston, F E; Laughlin, W S; Harper, A B; Ensroth, A E

    1982-08-01

    Growth patterns of body size, proportion, and composition were analyzed in 57 male and 56 female Eskimos from St. Lawrence Island in the Bering Sea, ranging in age from 1.23 through 19.82 years. Age-groups means for whites and blacks of the U.S. Health Examination Survey served as reference data. Relative to HES data, the Eskimo sample were shorter with lower values for leg length, while there were no differences from the reference values for sitting height. The Eskimos also had higher values of Quetelet's Index, the sitting height/height ratio, and the upper arm muscle circumference, while there were no differences in body weight or triceps skinfold thickness. Differences from the reference data were more pronounced in males than in females. The growth patterns for size and body proportion are in conformity with known relationships between morphology and climate.

  5. High Energy, Short Pulse Fiber Injection Lasers at Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Dawson, J W; Messerly, M J; Phan, H H; Crane, J K; Beach, R J; Siders, C W; Barty, C J

    2008-09-10

    A short pulse fiber injection laser for the Advanced Radiographic Capability (ARC) on the National Ignition Facility (NIF) has been developed at Lawrence Livermore National Laboratory (LLNL). This system produces 100 {micro}J pulses with 5 nm of bandwidth centered at 1053 nm. The pulses are stretched to 2.5 ns and have been recompressed to sub-ps pulse widths. A key feature of the system is that the pre-pulse power contrast ratio exceeds 80 dB. The system can also precisely adjust the final recompressed pulse width and timing and has been designed for reliable, hands free operation. The key challenges in constructing this system were control of the signal to noise ratio, dispersion management and managing the impact of self phase modulation on the chirped pulse.

  6. Assessment of Eligibility to National Register of Historic Places Building 431 Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Sullivan, M A; Ullrich, R A

    2003-05-07

    Lawrence Livermore National Laboratory (LLNL) proposes to demolish the original sections of Building 431 at its main site in Livermore, California. As this action will constitute an undertaking within the regulatory constraints of the National Historic Preservation Act, LLNL arranged for an assessment of the building's historic significance. This report provides a brief history of the magnetic fusion energy research activities housed in Building 431 and a historic assessment of the building. The final recommendation of the report is that, although Building 431 housed some significant breakthroughs in accelerator technology and magnetic mirror plasma confinement, it lacks integrity for the periods of significance of those developments. It is, therefore, not eligible to the National Register of Historic Places.

  7. Construction and operation of replacement hazardous waste handling facility at Lawrence Berkeley Laboratory. Environmental Assessment

    Energy Technology Data Exchange (ETDEWEB)

    1992-09-01

    The US Department of Energy (DOE) has prepared an environmental assessment (EA), DOE/EA-0423, for the construction and operation of a replacement hazardous waste handling facility (HWHF) and decontamination of the existing HWHF at Lawrence Berkeley Laboratory (LBL), Berkeley, California. The proposed facility would replace several older buildings and cargo containers currently being used for waste handling activities and consolidate the LBL`s existing waste handling activities in one location. The nature of the waste handling activities and the waste volume and characteristics would not change as a result of construction of the new facility. Based on the analysis in the EA, DOE has determined that the proposed action would not constitute a major Federal action significantly affecting the quality of the human environment within the meaning of the National Environmental Policy Act (NEPA) of 1969, 42 USC. 4321 et seq. Therefore, an environmental impact statement is not required.

  8. The Long-Term Corrosion Test Facility at the Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Fix, D V; Rebak, R B

    2007-03-21

    The long-term corrosion test facility (LTCTF) at the Lawrence Livermore National Laboratory (LLNL) consisted of 22 vessels that housed more than 7,000 corrosion test specimens from carbon steels to highly corrosion resistant materials such Alloy 22 and Ti Grade 7. The specimens from LTCTF range from standard weight-loss coupons to U-bend specimens for testing susceptibility to environmentally assisted cracking. Each vessel contained approximately 1000 liters of concentrated brines at 60 C or 90 C. The LTCTF started its operations in late 1996. The thousands of specimens from the LTCTF were removed in August-September 2006. The specimens are being catalogued and stored for future characterization. Previously removed specimens (e.g. 1 and 5 years) are also archived for further studies.

  9. Packaging design for Lawrence Berkeley National Laboratory high-resistivity CCDs

    Science.gov (United States)

    Stover, Richard J.; Brown, William E.; Robinson, Lloyd B.; Gilmore, D. K.; Wei, Mingzhi; Lockwood, Christopher

    2004-09-01

    The Lawrence Berkeley National Laboratory has been developing fully-depleted high resistivity CCDs. These CCDs exhibit very high red quantum efficiency, no red fringing, and very low lateral charge diffusion, making them good candidates for astronomical applications that require better red response or better point spread function than can typically be achieved with standard thinned CCDs. For the LBNL 2Kx4K CCD we have developed a four-side mosaic package fabricated from aluminum nitride. Our objectives have been to achieve a flatness of less than 10 micrometers peak-to-valley and a consistent final package thickness variation of 10 micrometers or less in a light-weight package. We have achieved the flatness objective, and we are working toward the thickness variation objective.

  10. Solid modeling research at Lawrence Livermore National Laboratory: 1982-1985

    Energy Technology Data Exchange (ETDEWEB)

    Kalibjian, J.R.

    1985-09-01

    The Lawrence Livermore National Laboratory has sponsored solid modeling research for the past four years to assess this new technology and to determine its potential benefits to the Nuclear Weapons Complex. We summarize here the results of five projects implemented during our effort. First, we have installed two solid modeler codes, TIPS-1 (Technical Information Processing System-1) and PADL-2 (Part and Assembly Description Language), on the Laboratory's CRAY-1 computers. Further, we have extended the geometric coverage and have enhanced the graphics capabilities of the TIPS-1 modeler. To enhance solid modeler performance on our OCTOPUS computer system, we have also developed a method to permit future use of the Laboratory's network video system to provide high-resolution, shaded images at users' locations. Finally, we have begun to implement code that will link solid-modeler data bases to finite-element meshing codes.

  11. Assessment of Eligibility to National Register of Historic Places Building 431 Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Sullivan, M A; Ullrich, R A

    2003-05-07

    Lawrence Livermore National Laboratory (LLNL) proposes to demolish the original sections of Building 431 at its main site in Livermore, California. As this action will constitute an undertaking within the regulatory constraints of the National Historic Preservation Act, LLNL arranged for an assessment of the building's historic significance. This report provides a brief history of the magnetic fusion energy research activities housed in Building 431 and a historic assessment of the building. The final recommendation of the report is that, although Building 431 housed some significant breakthroughs in accelerator technology and magnetic mirror plasma confinement, it lacks integrity for the periods of significance of those developments. It is, therefore, not eligible to the National Register of Historic Places.

  12. Title I conceptual design for Pit 6 landfill closure at Lawrence Livermore National Laboratory Site 300

    Energy Technology Data Exchange (ETDEWEB)

    MacDonnell, B.A.; Obenauf, K.S. [Golder Associates, Inc., Alameda, CA (United States)

    1996-08-01

    The objective of this design project is to evaluate and prepare design and construction documents for a closure cover cap for the Pit 6 Landfill located at Lawrence Livermore National Laboratory Site 300. This submittal constitutes the Title I Design (Conceptual Design) for the closure cover of the Pit 6 Landfill. A Title I Design is generally 30 percent of the design effort. Title H Design takes the design to 100 percent complete. Comments and edits to this Title I Design will be addressed in the Title II design submittal. Contents of this report are as follows: project background; design issues and engineering approach; design drawings; calculation packages; construction specifications outline; and construction quality assurance plan outline.

  13. Differential Expression and Clinical Significance of miR-328, miR-22 and miR-147 in Coronary Heart Disease Patients%miR-328、miR-147和miR-22在冠心病患者中的表达变化及临床意义

    Institute of Scientific and Technical Information of China (English)

    冯荣; 傅广; 黄树斌; 石顺华; 汤华; 王爱平

    2015-01-01

    目的 探讨冠心病患者血浆及外周血单个核细胞(PBMC) miR-328、miR-22、miR-147的表达变化及其临床意义.方法 筛选100例冠心病患者及相对健康对照组,采用实时荧光定量PCR技术检测各组血浆及PBMC中miR-328、miR-147、miR-22的表达水平.结果 冠心病患者血浆中miR-328、miR-22的表达较非冠心病患者明显升高,而miR-147的表达明显降低;在冠心病患者PBMC中,miR-328表达没有明显变化,miR-22表达略微增加,miR-147表达明显下降.在心肌梗死患者中,血浆中miR-328和miR-22表达水平明显增加,而miR-147表达明显下降;心肌梗死患者PBMC中miR-328表达略降低,miR-22表达增加,miR-147表达水平略有下降.结论 miR-328、miR-22及miR-147在冠心病患者中的差异表达,有望作为冠心病疾病中一种新的标记物或基因治疗靶点.

  14. 子宫内膜异位症中miR-143、miR-145和miR-126的差异表达%Differential expression of miR-143,miR-145 and miR-126 in endometriosis

    Institute of Scientific and Technical Information of China (English)

    郑冰冰; 徐超逸; 赵益萍; 韩灵芝; 虞楼超; 段萍

    2016-01-01

    目的 探索子宫内膜异位症(EMs)中miR-143、miR-145和miR-126的差异表达,以及不同月经周期的影响.方法 利用real time RT-PCR技术,比较miR-143、miR-145和miR-126在EMs患者在位内膜(EU,2例)、异位内膜(EC,14例)、配对EU+EC(14例),与非EMs患者内膜(EN,28例)中的表达差异;并分析EN和EC组不同月经周期对miR-143、miR-145和miR-126表达的影响.结果 不同月经周期的影响分析,发现仅EN标本中miR-143增生期表达量高于分泌期,差异有统计学意义(P<0.05).与EN相比,miR-143和miR-145在EU中均表达上调(P<0.05),miR-143、miR-145和miR-126在EC中均表达上调(P<0.01).配对EU-EC中,miR-143、miR-145和miR-126在EC中均表达上调(P<0.01).结论 miR-143、miR-145和miR-126在EMs中表达高于非EMs,在EC中表达高于EU.

  15. Haplotype distribution and evolutionary pattern of miR-17 and miR-124 families based on population analysis.

    Directory of Open Access Journals (Sweden)

    Li Guo

    Full Text Available BACKGROUND: MicroRNAs (miRNAs are small, endogenously expressed non-coding RNAs that regulate mRNAs post-transcriptionally. Previous studies have explored miRNA evolutionary trend, but evolutionary history and pattern in the miRNA world are still not fully clear. In the paper, we intended to analyze miRNA haplotype distribution and evolutionary network by analyzing miRNA sequences of miR-17 and miR-124 families across animal species as special populations. PRINCIPAL FINDINGS: 31 haplotypes were detected in miR-17 family while only 9 haplotypes were defined in miR-124 family. The complex miR-17 family was mainly distributed in vertebrates, but miR-124 was shared by more animal species from Caenorhabditis to Homo and had a wide distribution spectrum. Some haplotypes of the two miRNA families appeared discontinuous distributions across animals. Compared with a simple phylogenetic network in miR-124 family, miR-17 family indicated a complex network with some median vectors that might be lost ancestral or potential miRNA haplotypes. By analyzing different miRNAs across 12 animal species, we found these small RNAs showed different haplotype diversities, haplotype distributions and phylogenetic networks. CONCLUSIONS: Different miRNAs had quite different haplotype distributions and evolutionary patterns. Discontinuous distributions of miRNAs and median vectors in phylogenetic networks implied more members in the miRNA world. miRNA may be an excellent phylogenetic marker to discover its evolutionary history and pattern across the animal kingdom.

  16. Analysis of miR-205 and miR-155 expression in the blood of breast cancer patients

    Institute of Scientific and Technical Information of China (English)

    Jingjing Liu; Qixin Mao; Yan Liu; Xiaomeng Hao; Sheng Zhang; Jin Zhang

    2013-01-01

    The purpose of this study was to identify and validate circulating microRNAs (miRNAs) in human plasma for use as breast cancer (BC) biomarkers and to analyze their relationship to clinicopathologic features and its preliminary biological function.Genome-wide expression profiling of miRNAs in BC was investigated by microarray analysis.nmiR-155 was up-regulated greater than two-fold in BC compared with Normal Adjacent Tissue (NAT),whereas let-7b,miR-381,miR-10b,miR-125a-Sp,miR-335,miR-205 and miR-145 were down-regulated greater than two-fold.Our hypothesis was that circulating miRNAs are also present and differentially expressed in the serum of BC patients compared to controls.Using real-time PCR (RT-PCR),we analyzed miR-205 and miR-155 in archived serum from 30 participants,20 with breast cancer and 10 healthy people.miR-205 was down-regulated in BC patient serum while miR-155 was up-regulated.Furthermore,we analyzed the relationship between the expression levels of these two miRNAs and the clinicopathologic parameters of BC patients.High expression of miR155 was associated with clinical stage,molecular type,Ki-67 and p53 in BC patients (P<0.05).By contrast,we found no significant correlation between miR-205 and BC patient clinicopathologic parameters.Functional analysis showed that ectopic expression of miR-205 significantly inhibits cell proliferation and promotes apoptosis.miR-205 was down-regulated and miR-155 was up-regulated in BC patient serum.miR-155 was positive correlated with clinical stage and ki-67 and negatively correlated with p53 status.

  17. Differential expression of miR-139, miR-486 and miR-21 in breast cancer patients sub-classified according to lymph node status

    DEFF Research Database (Denmark)

    Rask, Lene; Balslev, Eva; Søkilde, Rolf;

    2014-01-01

    PURPOSE: Therapeutic decisions in breast cancer are increasingly guided by prognostic and predictive biomarkers. Non-protein-coding microRNAs (miRNAs) have recently been found to be deregulated in breast cancers and, in addition, to be correlated with several clinico-pathological features. One...... of the most consistently up-regulated miRNAs is miR-21. Here, we specifically searched for differentially expressed miRNAs in high-risk breast cancer patients as compared to low-risk breast cancer patients. In the same patients, we also compared miR-21 expression with the expression of its presumed target...... PTEN. METHODS: Both microarray and RT-qPCR techniques were used to assess miRNA expression levels in lymph node-positive and -negative human invasive ductal carcinoma tissues. Simultaneously, PTEN protein expression levels were assessed using immunohistochemistry. RESULTS: miR-486-5p and miR-139-5p...

  18. miR-221和miR-222在胃癌中的表达及临床意义%Expression of miR-221 and miR-222 in Gastric Cancer and its Clinical Significance

    Institute of Scientific and Technical Information of China (English)

    金晶; 闫竞一; 薛向阳; 武文一; 黄颖鹏; 朱冠保; 沈贤

    2011-01-01

    [Purpose] To investigate the expression of miR-221 and miR-222 in gastric cancer and its relationship with clinicopathologic features. [ Method ] The expression of miR-221 and miR-222 in 32 cases of gastric cancer tissues and their matched non-tumor adjacent tissues was examined by stem-loop RT-PCR. And the correlation with clinicopathologic features was investigated. [ Resuits] The expression of miR-221 and miR-222 in gastric cancer tissues was significantly higher than those in adjacent tissues (P<0.05). The ratio of miR-221/miR-222 expression had significant difference (P=0.05). The expression of miR-222 was related to differentiation (P=0.046); the expression of miR-221 was related to lymph node metastasis(P=0.013). [Conclusion] The expressions of miR-221 and miR-222 in gastric cancer markedly up regulation,they are important markers for gastric cancer,and they may play different effect with different stages in gastric cancer.%[目的]探讨miR-221和miR-222在胃癌中的表达及与临床病理特征的关系.[方法]应用茎环RT-PCR方法检测32例胃癌及癌旁胃黏膜组织中miR-221和miR-222表达,分析miR-221和miR-222表达与胃癌临床病理指标的关系.[结果]胃癌组织miR-221和miR-222表达明显高于对应的正常胃黏膜组织(P<0.05),且胃癌与正常胃黏膜组织中miR-221和miR-222比值也有显著性差异(P=0.05).miR-222表达与胃癌分化程度相关(P=0.046);miR-221表达与淋巴结转移相关(P=0.013).[结论]miR-221和miR-222在胃癌中表达明显上调是胃癌的重要标志.但两者在胃癌发生发展的不同阶段,可能发挥不同的效应.

  19. Reprogramming of miRNA networks in cancer and leukemia

    Science.gov (United States)

    Volinia, Stefano; Galasso, Marco; Costinean, Stefan; Tagliavini, Luca; Gamberoni, Giacomo; Drusco, Alessandra; Marchesini, Jlenia; Mascellani, Nicoletta; Sana, Maria Elena; Abu Jarour, Ramzey; Desponts, Caroline; Teitell, Michael; Baffa, Raffaele; Aqeilan, Rami; Iorio, Marilena V.; Taccioli, Cristian; Garzon, Ramiro; Di Leva, Gianpiero; Fabbri, Muller; Catozzi, Marco; Previati, Maurizio; Ambs, Stefan; Palumbo, Tiziana; Garofalo, Michela; Veronese, Angelo; Bottoni, Arianna; Gasparini, Pierluigi; Harris, Curtis C.; Visone, Rosa; Pekarsky, Yuri; de la Chapelle, Albert; Bloomston, Mark; Dillhoff, Mary; Rassenti, Laura Z.; Kipps, Thomas J.; Huebner, Kay; Pichiorri, Flavia; Lenze, Dido; Cairo, Stefano; Buendia, Marie-Annick; Pineau, Pascal; Dejean, Anne; Zanesi, Nicola; Rossi, Simona; Calin, George A.; Liu, Chang-Gong; Palatini, Jeff; Negrini, Massimo; Vecchione, Andrea; Rosenberg, Anne; Croce, Carlo M.

    2010-01-01

    We studied miRNA profiles in 4419 human samples (3312 neoplastic, 1107 nonmalignant), corresponding to 50 normal tissues and 51 cancer types. The complexity of our database enabled us to perform a detailed analysis of microRNA (miRNA) activities. We inferred genetic networks from miRNA expression in normal tissues and cancer. We also built, for the first time, specialized miRNA networks for solid tumors and leukemias. Nonmalignant tissues and cancer networks displayed a change in hubs, the most connected miRNAs. hsa-miR-103/106 were downgraded in cancer, whereas hsa-miR-30 became most prominent. Cancer networks appeared as built from disjointed subnetworks, as opposed to normal tissues. A comparison of these nets allowed us to identify key miRNA cliques in cancer. We also investigated miRNA copy number alterations in 744 cancer samples, at a resolution of 150 kb. Members of miRNA families should be similarly deleted or amplified, since they repress the same cellular targets and are thus expected to have similar impacts on oncogenesis. We correctly identified hsa-miR-17/92 family as amplified and the hsa-miR-143/145 cluster as deleted. Other miRNAs, such as hsa-miR-30 and hsa-miR-204, were found to be physically altered at the DNA copy number level as well. By combining differential expression, genetic networks, and DNA copy number alterations, we confirmed, or discovered, miRNAs with comprehensive roles in cancer. Finally, we experimentally validated the miRNA network with acute lymphocytic leukemia originated in Mir155 transgenic mice. Most of miRNAs deregulated in these transgenic mice were located close to hsa-miR-155 in the cancer network. PMID:20439436

  20. Effects of polycystic ovary syndrome (PCOS), sex hormones, and obesity on circulating miRNA-21, miRNA-27b, miRNA-103, and miRNA-155 expression.

    Science.gov (United States)

    Murri, Mora; Insenser, María; Fernández-Durán, Elena; San-Millán, José L; Escobar-Morreale, Héctor F

    2013-11-01

    MicroRNAs (miRNAs) are small, noncoding RNA sequences that negatively regulate gene expression at the post-transcriptional level. miRNA-21, miRNA-27b, miRNA-103, and miRNA-155 have been associated with metabolic disorders such as obesity and diabetes, which are also associated with polycystic ovary syndrome (PCOS). We aimed to evaluate the effects of sex, sex hormones, and PCOS and their interactions with obesity on the expression in the circulation of these miRNAs. This was a case-control study. The setting was an academic hospital. We included 12 control women, 12 patients with PCOS, and 12 men selected as to have similar body mass index (BMI) and age. Six subjects per group had normal weight (BMI obese (BMI ≥ 30 kg/m(2)). Blood samples were collected early in the morning after a 12-hour fast. We measured whole blood expression of miRNA-21, miRNA-27b, miRNA-103, and miRNA-155. Obesity significantly reduced the expression of miRNA-21, miRNA-27b, and miRNA-103. However, there was a significant interaction between obesity and the group of subjects in the expression of miRNA-21, miRNA-27b, miRNA-103, and miRNA-155 consisting of obesity reducing the expression of these miRNAs in control woman and men, but tending to increase their expression in women with PCOS. These differences paralleled those observed in serum T concentrations. The present results suggest that miRNAs that play an important role in metabolic and immune system processes are influenced by obesity and circulating androgen concentrations.

  1. miR-29b, miR-205 and miR-221 enhance chemosensitivity to gemcitabine in HuH28 human cholangiocarcinoma cells.

    Directory of Open Access Journals (Sweden)

    Kinya Okamoto

    Full Text Available BACKGROUND AND AIMS: Cholangiocarcinoma (CCA is highly resistant to chemotherapy, including gemcitabine (Gem treatment. MicroRNAs (miRNAs are endogenous, non-coding, short RNAs that can regulate multiple genes expression. Some miRNAs play important roles in the chemosensitivity of tumors. Here, we examined the relationship between miRNA expression and the sensitivity of CCA cells to Gem. METHODS: Microarray analysis was used to determine the miRNA expression profiles of two CCA cell lines, HuH28 and HuCCT1. To determine the effect of candidate miRNAs on Gem sensitivity, expression of each candidate miRNA was modified via either transfection of a miRNA mimic or transfection of an anti-oligonucleotide. Ontology-based programs were used to identify potential target genes of candidate miRNAs that were confirmed to affect the Gem sensitivity of CCA cells. RESULTS: HuCCT1 cells were more sensitive to Gem than were HuH28 cells, and 18 miRNAs were differentially expressed whose ratios over ± 2log2 between HuH28 and HuCCT1. Among these 18 miRNAs, ectopic overexpression of each of three downregulated miRNAs in HuH28 (miR-29b, miR-205, miR-221 restored Gem sensitivity to HuH28. Suppression of one upregulated miRNA in HuH28, miR-125a-5p, inhibited HuH28 cell proliferation independently to Gem treatment. Selective siRNA-mediated downregulation of either of two software-predicted targets, PIK3R1 (target of miR-29b and miR-221 or MMP-2 (target of miR-29b, also conferred Gem sensitivity to HuH28. CONCLUSIONS: miRNA expression profiling was used to identify key miRNAs that regulate Gem sensitivity in CCA cells, and software that predicts miRNA targets was used to identify promising target genes for anti-tumor therapies.

  2. miReader: Discovering Novel miRNAs in Species without Sequenced Genome.

    Directory of Open Access Journals (Sweden)

    Ashwani Jha

    Full Text Available Along with computational approaches, NGS led technologies have caused a major impact upon the discoveries made in the area of miRNA biology, including novel miRNAs identification. However, to this date all microRNA discovery tools compulsorily depend upon the availability of reference or genomic sequences. Here, for the first time a novel approach, miReader, has been introduced which could discover novel miRNAs without any dependence upon genomic/reference sequences. The approach used NGS read data to build highly accurate miRNA models, molded through a Multi-boosting algorithm with Best-First Tree as its base classifier. It was comprehensively tested over large amount of experimental data from wide range of species including human, plants, nematode, zebrafish and fruit fly, performing consistently with >90% accuracy. Using the same tool over Illumina read data for Miscanthus, a plant whose genome is not sequenced; the study reported 21 novel mature miRNA duplex candidates. Considering the fact that miRNA discovery requires handling of high throughput data, the entire approach has been implemented in a standalone parallel architecture. This work is expected to cause a positive impact over the area of miRNA discovery in majority of species, where genomic sequence availability would not be a compulsion any more.

  3. Oligoasthenoteratozoospermia and infertility in mice deficient for miR-34b/c and miR-449 loci.

    Directory of Open Access Journals (Sweden)

    Stefano Comazzetto

    2014-10-01

    Full Text Available Male fertility requires the continuous production of high quality motile spermatozoa in abundance. Alterations in all three metrics cause oligoasthenoteratozoospermia, the leading cause of human sub/infertility. Post-mitotic spermatogenesis inclusive of several meiotic stages and spermiogenesis (terminal spermatozoa differentiation are transcriptionally inert, indicating the potential importance for the post-transcriptional microRNA (miRNA gene-silencing pathway therein. We found the expression of miRNA generating enzyme Dicer within spermatogenesis peaks in meiosis with critical functions in spermatogenesis. In an expression screen we identified two miRNA loci of the miR-34 family (miR-34b/c and miR-449 that are specifically and highly expressed in post-mitotic male germ cells. A reduction in several miRNAs inclusive of miR-34b/c in spermatozoa has been causally associated with reduced fertility in humans. We found that deletion of both miR34b/c and miR-449 loci resulted in oligoasthenoteratozoospermia in mice. MiR-34bc/449-deficiency impairs both meiosis and the final stages of spermatozoa maturation. Analysis of miR-34bc-/-;449-/- pachytene spermatocytes revealed a small cohort of genes deregulated that were highly enriched for miR-34 family target genes. Our results identify the miR-34 family as the first functionally important miRNAs for spermatogenesis whose deregulation is causal to oligoasthenoteratozoospermia and infertility.

  4. Value of combined detection of serum miR-21,miR-195 and miR-222 in the diagnosis of early breast cancer

    Institute of Scientific and Technical Information of China (English)

    Wen-Zhao Zhang; Ying Li; De-Xiang Wu

    2016-01-01

    Objective:To study level of miRNA in the serum of early breast carcinoma patients and to evaluate the clinical diagnostic value of combined detection of early breast carcinoma. Methods:A total of 54 cases of early breast carcinoma, 58 cases of benign breast diseases and 70 cases of healthy physical examination women were selected as the research subjects, to analysis serum miR-21, miR-195 and miR-222 levels by fluorescence quantitative PCR method and to analyze the diagnostic value of single and combined detection in early breast cancer by receiver operating characteristic curve. Results:The relative expression levels of miR-21, miR-222 and miR-195 in early breast carcinoma patients were significantly higher than those in benign breast disease and healthy controls;there was no significant difference in the relative expression of miR-21, miR-222 and miR-195 in the benign breast disease group and healthy control group;receiver operating characteristic curve analysis showed that AUC of miR-21, miR-195 and miR-222 in the diagnosis of early breast carcinoma were 0.805, 0.86 and 0.848 respectively, the sensitivity were 63.3%, 70.0%and 70.0%, and the specificity were 86.7%, 93.3%and 90.0%;AUC, sensitivity and specificity of the combined detection were 0.974, 93.3%and 96.7%respectively. Conclusion:miR-21, miR-195 and miR-222 levels in serum of patients with early breast carcinoma rise, the combined detection of the 3 indicators have a high diagnostic value for early breast carcinoma, and contribute to early breast carcinoma screening and diagnosis.

  5. Role of miR-27a, miR-181a and miR-20b in gastric cancer hypoxia-induced chemoresistance

    Science.gov (United States)

    Danza, Katia; Silvestris, Nicola; Simone, Giovanni; Signorile, Michele; Saragoni, Luca; Brunetti, Oronzo; Monti, Manlio; Mazzotta, Annalisa; De Summa, Simona; Mangia, Anita; Tommasi, Stefania

    2016-01-01

    ABSTRACT Despite the search for new therapeutic strategies for gastric cancer (GC), there is much evidence of progression due to resistance to chemotherapy. Multidrug resistance (MDR) is the ability of cancer cells to survive after exposure to chemotherapeutic agents. The involvement of miRNAs in the development of MDR has been well described but miRNAs able to modulate the sensitivity to chemotherapy by regulating hypoxia signaling pathways have not yet been fully addressed in GC. Our aim was to analyze miR-20b, miR-27a and miR-181a expression with respect to (epirubicin/oxaliplatin/capecitabine (EOX)) chemotherapy regimen in a set of GC patients, in order to investigate whether miRNAs deregulation may influence GC MDR also via hypoxia signaling modulation. Cancer biopsy were obtained from 21 untreated HER2 negative advanced GC patients, retrospectively analyzed. All patients received a first-line chemotherapy (EOX) regimen. MirWalk database was used to identify miR-27a, miR-181a and miR-20b target genes. The expression of miRNAs and of HIPK2, HIF1A and MDR1 genes were detected by real-time PCR. HIPK2 localization was assessed by immunohistochemistry. Our data showed the down-regulation of miR-20b, miR-27a, miR-181a concomitantly to higher levels of MDR1, HIF1A and HIPK2 genes in GC patients with a progressive disease respect to those with a disease control rate. Moreover, immunohistochemistry assay highlighted a higher cytoplasmic HIPK2 staining, suggesting a different role for it. We showed that aberrant expression of miR-20b, miR27a and miR-181a was associated with chemotherapeutic response in GC through HIF1A, MDR1 and HIPK2 genes modulation, suggesting a possible novel therapeutic strategy. PMID:26793992

  6. Role of miR-27a, miR-181a and miR-20b in gastric cancer hypoxia-induced chemoresistance.

    Science.gov (United States)

    Danza, Katia; Silvestris, Nicola; Simone, Giovanni; Signorile, Michele; Saragoni, Luca; Brunetti, Oronzo; Monti, Manlio; Mazzotta, Annalisa; De Summa, Simona; Mangia, Anita; Tommasi, Stefania

    2016-04-02

    Despite the search for new therapeutic strategies for gastric cancer (GC), there is much evidence of progression due to resistance to chemotherapy. Multidrug resistance (MDR) is the ability of cancer cells to survive after exposure to chemotherapeutic agents. The involvement of miRNAs in the development of MDR has been well described but miRNAs able to modulate the sensitivity to chemotherapy by regulating hypoxia signaling pathways have not yet been fully addressed in GC. Our aim was to analyze miR-20b, miR-27a and miR-181a expression with respect to (epirubicin/oxaliplatin/capecitabine (EOX)) chemotherapy regimen in a set of GC patients, in order to investigate whether miRNAs deregulation may influence GC MDR also via hypoxia signaling modulation. Cancer biopsy were obtained from 21 untreated HER2 negative advanced GC patients, retrospectively analyzed. All patients received a first-line chemotherapy (EOX) regimen. MirWalk database was used to identify miR-27a, miR-181a and miR-20b target genes. The expression of miRNAs and of HIPK2, HIF1A and MDR1 genes were detected by real-time PCR. HIPK2 localization was assessed by immunohistochemistry. Our data showed the down-regulation of miR-20b, miR-27a, miR-181a concomitantly to higher levels of MDR1, HIF1A and HIPK2 genes in GC patients with a progressive disease respect to those with a disease control rate. Moreover, immunohistochemistry assay highlighted a higher cytoplasmic HIPK2 staining, suggesting a different role for it. We showed that aberrant expression of miR-20b, miR27a and miR-181a was associated with chemotherapeutic response in GC through HIF1A, MDR1 and HIPK2 genes modulation, suggesting a possible novel therapeutic strategy.

  7. Early Sámi visual artists - Western fine art meets Sámi culture

    Directory of Open Access Journals (Sweden)

    Tuija Hautala-Hirvioja

    2014-04-01

    Full Text Available Johan Turi (1854–1936, Nils Nilsson Skum (1872–1951 and John Savio (1902–1938 were among the first Sámi visual artists. The production of their art work occurred between the 1910s and the early 1950s. Sámi aesthetics had its basis in folklore, i.e., handicraft or duodji, which did not follow the principle of art for art’s sake but combined beauty and practicality. Art was part of community life. Not until the 1970s was the word daidda, which is Finnish in origin and which means “art”, adopted into the Sámi language. Turi and Skum became famous through their books. They drew and wrote in order to pass the traditional knowledge of their people on to succeeding generations. They also wanted to introduce Sámi life and culture to non-Sámi people. One typical feature of their work is that they depicted Sáminess in a realistic way and sought to strengthen and preserve the Sámi identity through their art. In Turi and Skum’s work, both the documentation of community life and their own personal expression were strongly present and equally important; for this reason their pictures and texts have both practical and aesthetic dimensions. They did not attend school and were self-taught artists. The third pioneer of Sámi visual arts was John Savio, who, unlike the other two, attended secondary school and studied visual arts both independently and under the guidance of a mentor. He expressively combined Western ways of depiction with Sámi subjects. My article examines what made these early Sámi artists change over from Sámi handicraft, duodji, to Western visual arts, how they used Western pictorial conventions in dealing with their Sámi subjects, and the significance of their art for Sámi identity and culture. They lived and worked under cross pressure: the first few decades of the 20th century were characterized by racial theories that denigrated Sámi people, and the period following World War II was marked by demands for

  8. A Medical Revolution That Could...: The Work of the PROMIS Laboratory and Lawrence L. Weed, M.D.

    Science.gov (United States)

    Cook, Gordon

    The problem-oriented medical record, developed by Lawrence L. Weed, M.D., involves an interactive computer system and has been operational on two wards at the University Hospital in Burlington, Vermont, a teaching hospital. The Problem-Oriented Medical Information System (PROMIS) consists of terminals that feed into the central memory units; the…

  9. A spatiotemporal model for snow crab (Chionoecetes opilio) stock size in the southern Gulf of St. Lawrence

    DEFF Research Database (Denmark)

    Cadigan, Noel G.; Wade, Elmer; Nielsen, Anders

    2017-01-01

    We develop a high-resolution spatiotemporal model of stock size and harvest rates for snow crab (Chionoecetes opilio) in the southern Gulf of St. Lawrence, which supports an economically important fishery off the east coast of Canada. It is a spatial and weekly model during 1997–2014 that utilize...

  10. A practical MRI grading system for osteoarthritis of the knee: Association with Kellgren–Lawrence radiographic scores

    Energy Technology Data Exchange (ETDEWEB)

    Park, Hee-Jin, E-mail: parkhiji@gmail.com [Department of Radiology, Sungkyunkwan University School of Medicine, Kangbuk Samsung Hospital, #108 Pyung-dong, Jongno-gu, Seoul 110-746 (Korea, Republic of); Department of Radiology, Kangwon National University School of Medicine, Baengnyeong-ro 156, Chuncheon-Si, Gangwon-Do Kangwon National University Hospital 200-722 (Korea, Republic of); Kim, Sam Soo, E-mail: samskim@kangwon.ac.kr [Department of Radiology, Kangwon National University School of Medicine, Baengnyeong-ro 156, Chuncheon-Si, Gangwon-Do Kangwon National University Hospital 200-722 (Korea, Republic of); Lee, So-Yeon, E-mail: parkhiji@kwandong.ac.kr [Department of Radiology, Sungkyunkwan University School of Medicine, Kangbuk Samsung Hospital, #108 Pyung-dong, Jongno-gu, Seoul 110-746 (Korea, Republic of); Park, Noh-Hyuck, E-mail: nhpark904@kwandong.ac.kr [Department of Radiology, Myongji Hospital, Kwandong University, College of Medicine, 697-24 Hwajung-dong, Dukyang-ku, Koyang, Kyunggi 412-270 (Korea, Republic of); Park, Ji-Yeon, E-mail: zzzz3@hanmail.net [Department of Radiology, Myongji Hospital, Kwandong University, College of Medicine, 697-24 Hwajung-dong, Dukyang-ku, Koyang, Kyunggi 412-270 (Korea, Republic of); Choi, Yoon-Jung, E-mail: yoonchoi99@gmail.com [Department of Radiology, Sungkyunkwan University School of Medicine, Kangbuk Samsung Hospital, #108 Pyung-dong, Jongno-gu, Seoul 110-746 (Korea, Republic of); Jeon, Hyun-Jun, E-mail: ostrich-13@hanmail.net [Department of Occupational Medicine, Dongsan Medical Center, Keimyung University School of Medicine, 194 Dongsan-Dong, Jung-ku, Taegu (Korea, Republic of)

    2013-01-15

    Purpose: To propose a reproducible and constant MR grading system for osteoarthritis of the knee joint that provides high interobserver and intraoberver agreement and that does not require complicated calculation procedures. Materials and methods: This retrospective study sample included 44 men and 65 women who underwent both MRI and plain radiography of the knee at our institution. All patients were older than 50 years of age (mean 57.7) and had clinically suspected osteoarthritis of the knee. The standard of 4 grades on the MR grade scale was based mainly on cartilage injury and additional findings. Kellgren–Lawrence grades were assessed for the same patient group. The relationship between the results was determined. Statistical analyses were performed including kappa statistics, categorical regression analysis and nonparametric correlation analysis. Results: The interobserver and intraoberver agreements between the two readers in the grading of osteoarthritis were found to be almost perfect. Interobserver and intraobserver agreements were slightly lower for the MR grading system than for the Kellgren–Lawrence grading scale. The correlation between the MR grade and Kellgren–Lawrence grade was very high and did not differ with patient age. The MR grades were highly correlated with the Kellgren–Lawrence grades and showed excellent interobserver and intraobserver agreements. Conclusion: This new MR grading system for osteoarthritis of the knee joint is reproducible and may be helpful for the grading of osteoarthritis of the knee without requiring reference to plain radiography.

  11. 78 FR 40260 - International Joint Commission: Public Comment on a Proposal for Lake Ontario and St. Lawrence...

    Science.gov (United States)

    2013-07-03

    ... International Joint Commission: Public Comment on a Proposal for Lake Ontario and St. Lawrence River Regulation The International Joint Commission (IJC) is inviting the public to comment on a proposal for managing... mail to either of the following addresses: International Joint Commission Secretary, U.S. Section,...

  12. 75 FR 8428 - The Indiana Rail Road Company-Abandonment Exemption-in Martin and Lawrence Counties, IN; CSX...

    Science.gov (United States)

    2010-02-24

    ... Mayor; Douglas England, City of New Albany Mayor; David Branneman, Lawrence County Tourism Commission... posted on the Board's Web site. This action will not significantly affect either the quality of the human environment or the conservation of energy resources. Dated: February 18, 2010. By the Board, Rachel...

  13. Lawrence Lessig, The architecture of access to scientific knowledge: just how badly we have messed this up

    Directory of Open Access Journals (Sweden)

    Maria Chiara Pievatolo

    2011-06-01

    Full Text Available E’ una conferenza tenuta da Lawrence Lessig al Cern di Ginevra il 18 aprile di quest’anno. Il suo video è disponibile presso il Cern Document Server; a partire da qui si possono trovare i sottotitoli in inglese e in altre lingue, compresa quella italiana. Lessig esordisce proponendo due impressioni. La prima è un effetto chiamato [...

  14. 75 FR 1010 - CSX Transportation, Inc.-Discontinuance of Service Exemption-in Clark, Floyd, Lawrence, Orange...

    Science.gov (United States)

    2010-01-07

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF TRANSPORTATION Surface Transportation Board CSX Transportation, Inc.--Discontinuance of Service Exemption--in Clark, Floyd, Lawrence, Orange, and Washington Counties, IN On December 18, 2009, CSX Transportation, Inc. (CSXT) filed with the Board a petition under...

  15. Radiographic evaluation of posttraumatic osteoarthritis of the ankle : The Kellgren-Lawrence scale is reliable and correlates with clinical symptoms

    NARCIS (Netherlands)

    Holzer, N.; Salvo, D.; Marijnissen, A. C A; Vincken, K. L.; Ahmad, A. C.; Serra, E.; Hoffmeyer, P.; Stern, R.; Lübbeke, A.; Assal, M.

    2015-01-01

    Objective: To assess reliability and construct validity of the Kellgren-Lawrence (K&L) scale in posttraumatic ankle osteoarthritis (OA); additionally evaluate the validity of including tibiotalar tilting in the scale. Method: One-hundred and fifty ankle radiographs (75 patients, unilateral malleolar

  16. Immune functions in the Fisher rat fed beluga whale (Delphinapterus leucas) blubber from the contaminated St. Lawrence estuary

    Energy Technology Data Exchange (ETDEWEB)

    Lapierre, P.; Guise, S. De [Univ. du Quebec, Montreal, Quebec (Canada). Dept. des Sciences Biologiques; Muir, D.C.G. [Freshwater Inst., Winnipeg, Manitoba (Canada); Norstrom, R. [Environment Canada, Hull, Quebec (Canada); Beland, P. [St. Lawrence National Inst. of Ecotoxicology, Montreal, Quebec (Canada); Fournier, M. [Univ. du Quebec, Montreal, Quebec (Canada). Dept. des Sciences Biologiques]|[INRS-Sante, Pointe-Claire, Quebec (Canada)]|[IML, Mont Joli, Quebec (Canada). Dept. of Fisheries and Oceans

    1999-02-01

    In order to assess the immunotoxic potential of food naturally contaminated with PCBs and other organohalogens, Fisher rats were fed a diet in which the lipids originated from the blubber of either a highly polluted St. Lawrence beluga or a relatively uncontaminated Arctic beluga. After a period of 2 months, different immune functions were evaluated, including lymphoblastic transformation, natural killer cell activity, plaque-forming cells, phagocytosis, oxidative burst, and immunophenotyping. For all assays, rats fed at St. Lawrence beluga blubber diet or a mixture of Arctic and St. Lawrence beluga blubber diet were not different from control rats fed a diet containing Arctic beluga blubber. These results are inconsistent with the well-known immunosuppressive effects of organochlorines in numerous species and with the lesions suggestive of organochlorine-related immunosuppression that are observed in St. Lawrence belugas. The lack of observable immunotoxic effects in rats fed contaminated beluga blubber might be explained by antagonistic effects in the organohalogen mixture, by a response specific to the rat, by a strain-related lack of sensitivity to organochlorines, or by insufficient dose due to the shortness of the exposure period or the route of exposure.

  17. Review Essay: They Had No Voice by Denny Abbott and Working for Peace and Justice by Lawrence S. Wittner

    Directory of Open Access Journals (Sweden)

    Jyl Lynn Felman

    2014-02-01

    Full Text Available Book Review comparing and contrasting the memoirs They Had No Voice by Denny Abbott and Working For Peace and Justice by Lawrence S. Wittner. Topics discussed include how the personal becomes political; working for social justice locally and globally; the disarmament movement, 1960's activism, and the omission of the feminist movement from both memoirs.

  18. Expression profiles of miRNAs and involvement of miR-100 and miR-34 in regulation of cell cycle arrest in Artemia.

    Science.gov (United States)

    Zhao, Ling-Ling; Jin, Feng; Ye, Xiang; Zhu, Lin; Yang, Jin-Shu; Yang, Wei-Jun

    2015-09-01

    Regulation of the cell cycle is complex but critical for proper development, reproduction and stress resistance. To survive unfavourable environmental conditions, the crustacean Artemia produces diapause embryos whose metabolism is maintained at extremely low levels. In the present study, the expression profiles of miRNAs during Artemia diapause entry and termination were characterized using high-throughput sequencing. A total of 13 unclassified miRNAs and 370 miRNAs belonging to 87 families were identified; among them, 107 were differentially expressed during diapause entry and termination. We focused on the roles of two of these miRNAs, miR-100 and miR-34, in regulating cell cycle progression; during the various stages of diapause entry, these miRNAs displayed opposing patterns of expression. A functional analysis revealed that miR-100 and miR-34 regulate the cell cycle during diapause entry by targeting polo-like kinase 1 (PLK1), leading to activation of the mitogen-activated protein kinase kinase-extracellular signal-regulated kinase-ribosomal S6 kinase 2 (MEK-ERK-RSK2) pathway and cyclin K, leading to suppression of RNA polymerase II (RNAP II) activity respectively. The findings presented in the present study provide insights into the functions of miR-100 and miR-34 and suggest that the expression profiles of miRNAs in Artemia can be used to characterize their functions in cell cycle regulation.

  19. Discovery of miRNAs and Their Corresponding miRNA Genes in Atlantic Cod (Gadus morhua: Use of Stable miRNAs as Reference Genes Reveals Subgroups of miRNAs That Are Highly Expressed in Particular Organs.

    Directory of Open Access Journals (Sweden)

    Rune Andreassen

    Full Text Available Atlantic cod (Gadus morhua is among the economically most important species in the northern Atlantic Ocean and a model species for studying development of the immune system in vertebrates. MicroRNAs (miRNAs are an abundant class of small RNA molecules that regulate fundamental biological processes at the post-transcriptional level. Detailed knowledge about a species miRNA repertoire is necessary to study how the miRNA transcriptome modulate gene expression. We have therefore discovered and characterized mature miRNAs and their corresponding miRNA genes in Atlantic cod. We have also performed a validation study to identify suitable reference genes for RT-qPCR analysis of miRNA expression in Atlantic cod. Finally, we utilized the newly characterized miRNA repertoire and the dedicated RT-qPCR method to reveal miRNAs that are highly expressed in certain organs.The discovery analysis revealed 490 mature miRNAs (401 unique sequences along with precursor sequences and genomic location of the miRNA genes. Twenty six of these were novel miRNA genes. Validation studies ranked gmo-miR-17-1-5p or the two-gene combination gmo-miR25-3p and gmo-miR210-5p as most suitable qPCR reference genes. Analysis by RT-qPCR revealed 45 miRNAs with significantly higher expression in tissues from one or a few organs. Comparisons to other vertebrates indicate that some of these miRNAs may regulate processes like growth, lipid metabolism, immune response to microbial infections and scar damage repair. Three teleost-specific and three novel Atlantic cod miRNAs were among the differentially expressed miRNAs.The number of known mature miRNAs was considerably increased by our identification of miRNAs and miRNA genes in Atlantic cod. This will benefit further functional studies of miRNA expression using deep sequencing methods. The validation study showed that stable miRNAs are suitable reference genes for RT-qPCR analysis of miRNA expression. Applying RT-qPCR we have identified

  20. MI Gap Clearing Kicker Magnet Design Review

    Energy Technology Data Exchange (ETDEWEB)

    Jensen, Chris; /Fermilab

    2008-10-01

    The kicker system requirements were originally conceived for the NOvA project. NOvA is a neutrino experiment located in Minnesota. To achieve the desired neutrino flux several upgrades are required to the accelerator complex. The Recycler will be used as a proton pre-injector for the Main Injector (MI). As the Recycler is the same size as the MI, it is possible to do a single turn fill ({approx}11 {micro}sec), minimizing the proton injection time in the MI cycle and maximizing the protons on target. The Recycler can then be filled with beam while the MI is ramping to extract beam to the target. To do this requires two new transfer lines. The existing Recycler injection line was designed for 10{pi} pbar beams, not the 20{pi} proton beams we anticipate from the Booster. The existing Recycler extraction line allows for proton injection through the MI, while we want direct injection from the Booster. These two lines will be decommissioned. The new injection line from the MI8 line into the Recycler will start at 848 and end with injection kickers at RR104. The new extraction line in the RR30 straight section will start with a new extraction kicker at RR232 and end with new MI injection kickers at MI308. Finally, to reduce beam loss activation in the enclosure, a new gap clearing kicker will be used to extract uncaptured beam created during the slip stack injection process down the existing dump line. It was suggested that the MI could benefit from this type of system immediately. This led to the early installation of the gap clearing system in the MI, followed by moving the system to Recycler during NOvA. The specifications also changed during this process. Initially the rise and fall time requirements were 38 ns and the field stability was {+-}1%. The 38 ns is based on having a gap of 2 RF buckets between injections. (There are 84 RF buckets that can be filled from the Booster for each injection, but 82 would be filled with beam. MI and Recycler contain 588 RF buckets

  1. Identification of ovarian cancer metastatic miRNAs.

    Directory of Open Access Journals (Sweden)

    Souriya Vang

    Full Text Available Serous epithelial ovarian cancer (EOC patients often succumb to aggressive metastatic disease, yet little is known about the behavior and genetics of ovarian cancer metastasis. Here, we aim to understand how omental metastases differ from primary tumors and how these differences may influence chemotherapy. We analyzed the miRNA expression profiles of primary EOC tumors and their respective omental metastases from 9 patients using miRNA Taqman qPCR arrays. We find 17 miRNAs with differential expression in omental lesions compared to primary tumors. miR-21, miR-150, and miR-146a have low expression in most primary tumors with significantly increased expression in omental lesions, with concomitant decreased expression of predicted mRNA targets based on mRNA expression. We find that miR-150 and miR-146a mediate spheroid size. Both miR-146a and miR-150 increase the number of residual surviving cells by 2-4 fold when challenged with lethal cisplatin concentrations. These observations suggest that at least two of the miRNAs, miR-146a and miR-150, up-regulated in omental lesions, stimulate survival and increase drug tolerance. Our observations suggest that cancer cells in omental tumors express key miRNAs differently than primary tumors, and that at least some of these microRNAs may be critical regulators of the emergence of drug resistant disease.

  2. MDRL lncRNA regulates the processing of miR-484 primary transcript by targeting miR-361.

    Directory of Open Access Journals (Sweden)

    Kun Wang

    2014-07-01

    Full Text Available Long noncoding RNAs (lncRNAs are emerging as new players in gene regulation, but whether lncRNAs operate in the processing of miRNA primary transcript is unclear. Also, whether lncRNAs are involved in the regulation of the mitochondrial network remains to be elucidated. Here, we report that a long noncoding RNA, named mitochondrial dynamic related lncRNA (MDRL, affects the processing of miR-484 primary transcript in nucleus and regulates the mitochondrial network by targeting miR-361 and miR-484. The results showed that miR-361 that predominantly located in nucleus can directly bind to primary transcript of miR-484 (pri-miR-484 and prevent its processing by Drosha into pre-miR-484. miR-361 is able to regulate mitochondrial fission and apoptosis by regulating miR-484 levels. In exploring the underlying molecular mechanism by which miR-361 is regulated, we identified MDRL and demonstrated that it could directly bind to miR-361 and downregulate its expression levels, which promotes the processing of pri-miR-484. MDRL inhibits mitochondrial fission and apoptosis by downregulating miR-361, which in turn relieves inhibition of miR-484 processing by miR-361. Our present study reveals a novel regulating model of mitochondrial fission program which is composed of MDRL, miR-361 and miR-484. Our work not only expands the function of the lncRNA pathway in gene regulation but also establishes a new mechanism for controlling miRNA expression.

  3. miR-10b, miR-26a, miR-146a And miR-153 Expression in Triple Negative Vs Non Triple Negative Breast Cancer: Potential Biomarkers.

    Science.gov (United States)

    Fkih M'hamed, Insaf; Privat, Maud; Trimeche, Mounir; Penault-Llorca, Frédérique; Bignon, Yves-Jean; Kenani, Abderraouf

    2017-01-18

    MicroRNAs (miRNAs) are small non-coding RNAs composed of 18-25 nucleotides that can post-transcriptionally regulate gene expression and have key regulatory roles in cancer, acting as both oncogenes and tumor suppressors. About 1000 genes in humans encode miRNAs, which account for approximately 3% of the human genome, and up to 30% of human protein coding genes may be regulated by miRNAs. The objective of this article is to evaluate the expression profile of four miRNAs previously implicated in triple negative breast cancer: miR-10b, miR-26a, miR-146a and miR-153, and to determine their possible interaction in triple negative and non triple negative breast cancer based on clinical outcome and the expression of BRCA1. 24 triple-negative and 13 non triple negative breast cancer cases, were studied by q-RT-PCR and immunohistochemistry to determine the expression of the four studied miRNAs and the BRCA1 protein, respectively. We observed that the BRCA1 protein was absent in 62.5% of the triple negative cases. Besides, the miR-146a and miR-26a were over expressed in triple negative breast cancer. These two miRNAs, miR-10b and miR-153 were significantly associated to lymph node metastases occurrence in triple negative breast carcinoma. All the analyzed microRNAs were not associated with the expression of BRCA1 in our conditions. Our work provides evidence that miR-146a, miR-26a, miR-10b and miR-153 could be defined as biomarkers in triple negative breast cancer to predict lymph node metastases (LNM).

  4. Differentially expressed miRNAs in trisomy 21 placentas.

    Science.gov (United States)

    Svobodová, Iveta; Korabečná, Marie; Calda, Pavel; Břešťák, Miroslav; Pazourková, Eva; Pospíšilová, Šárka; Krkavcová, Miroslava; Novotná, Michaela; Hořínek, Aleš

    2016-08-01

    Molecular pathogenesis of Down syndrome (DS) is still incompletely understood. Epigenetic mechanisms, including miRNAs gene expression regulation, belong to potential influencing factors. The aims of this study were to compare miRNAs expressions in placentas with normal and trisomic karyotype and to associate differentially expressed miRNAs with concrete biological pathways. A total of 80 CVS samples - 41 with trisomy 21 and 39 with normal karyotype - were included in our study. Results obtained in the pilot study using real-time PCR technology and TaqMan Human miRNA Array Cards were subsequently validated on different samples using individual TaqMan miRNA Assays. Seven miRNAs were verified as upregulated in DS placentas (miR-99a, miR-542-5p, miR-10b, miR-125b, miR-615, let-7c and miR-654); three of these miRNAs are located on chromosome 21 (miR-99a, miR-125b and let-7c). Many essential biological processes, transcriptional regulation or apoptosis, were identified as being potentially influenced by altered miRNA levels. Moreover, miRNAs overexpressed in DS placenta apparently regulate genes involved in placenta development (GJA1, CDH11, EGF, ERVW-1, ERVFRD-1, LEP or INHA). These findings suggest the possible participation of miRNAs in Down syndrome impaired placentation and connected pregnancy pathologies. © 2016 John Wiley & Sons, Ltd. © 2016 John Wiley & Sons, Ltd.

  5. TERRAIN, MONROE COUNTY, TN, USA

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Terrain data, as defined in FEMA Guidelines and Specifications, Appendix N: Data Capture Standards, describes the digital topographic data that was used to create...

  6. FLOODPLAIN, MONROE COUNTY, PENNSYLVANIA, USA

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — The Floodplain Mapping/Redelineation study deliverables depict and quantify the flood risks for the study area. The primary risk classifications used are the...

  7. FLOODPLAIN, Monroe County, Michigan, USA

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — The Floodplain Mapping/Redelineation study deliverables depict and quantify the flood risks for the study area. The primary risk classifications used are the...

  8. The regulation roles of miR-125b, miR-221 and miR-27b in porcine Salmonella infection signalling pathway.

    Science.gov (United States)

    Yao, Min; Gao, Weihua; Yang, Jun; Liang, Xiongyan; Luo, Jingbo; Huang, Tinghua

    2016-08-01

    miRNAs are non-coding RNA molecules typically 18-22 nucleotides long that can suppress the expression of their target genes. Several laboratories have attempted to identify miRNAs from the pig that are involved in Salmonella infection. These bioinformatics strategies using the newly available genomic sequence are generally successful. Here, we report an in silico identification of miRNAs in pig focusing on the Salmonella infection pathway, and further investigated the differential expression of those miRNAs by quantitative real-time PCR during pre- and post-natal stage of Salmonella inoculation from the peripheral blood of commercially breed pigs. We identified 29 miRNAs that have predicted targets in the Salmonella infection pathway and nine of them were not yet described in pig. In addition, the expression of nine selected miRNAs was validated in the peripheral blood by northern blotting. Through expression analyses, differences were found between pre- and post-natal stages of Salmonella inoculation for miR-221, miR-125b and miR-27b-all of them were suppressed 2 days after Salmonella inoculation. The predicted targets of those three miRNAs were validated by luciferase reporter assays. We show that FOS is a direct target of miR-221, miR-125b can suppress MAPK14, and miR-27b can target IFNG. These findings will be helpful in understanding the function and processing of these miRNAs in Salmonella infection. The miRNA differentially expressed in the peripheral blood of commercial breed pigs suggest that it can be used as genetic markers for salmonella infection resistance in pigs.

  9. Identification of miR414 and expression analysis of conserved miRNAs from Stevia rebaudiana.

    Science.gov (United States)

    Guleria, Praveen; Yadav, Sudesh Kumar

    2011-12-01

    MicroRNAs (miRNAs) usually contain 19-24 nucleotides and have been identified as important eukaryotic gene regulators. Applications of various computational approaches have simplified the task by predicting miRNAs from available sequence data sources. In this study, we identified a conserved miR414 from a computational analysis of EST sequence data available from Stevia rebaudiana. In addition, we also identified six conserved miRNAs namely miR169, miR319, miR414, miR164, miR167 and miR398 using stem-loop RT-PCR analysis. Hence, miR414 was commonly identified using both methods. The expression analysis of these miRNAs documented their roles in growth and development of Stevia. Furthermore, the detected miRNAs were found to target genes involved in plant growth, development, metabolism and signal transduction. This is the first study reporting these conserved miRNAs and their expression in Stevia.

  10. Fluoxetine Increases the Expression of miR-572 and miR-663a in Human Neuroblastoma Cell Lines

    Science.gov (United States)

    Mundalil Vasu, Mahesh; Anitha, Ayyappan; Takahashi, Taro; Thanseem, Ismail; Iwata, Keiko; Asakawa, Tetsuya; Suzuki, Katsuaki

    2016-01-01

    Evidence suggests neuroprotective effects of fluoxetine, a selective serotonin reuptake inhibitor (SSRI), on the developed neurons in the adult brain. In contrast, the drug may be deleterious to immature or undifferentiated neural cells, although the mechanism is unclear. Recent investigations have suggested that microRNAs (miRNA) may be critical for effectiveness of psychotropic drugs including SSRI. We investigated whether fluoxetine could modulate expressions of neurologically relevant miRNAs in two neuroblastoma SK-N-SH and SH-SY5Y cell lines. Initial screening results revealed that three (miR-489, miR-572 and miR-663a) and four (miR-320a, miR-489, miR-572 and miR-663a) miRNAs were up-regulated in SK-N-SH cells and SH-SY5Y cells, respectively, after 24 hours treatment of fluoxetine (1–25 μM). Cell viability was reduced according to the dose of fluoxetine. The upregulation of miR-572 and miR-663a was consistent in both the SH-SY5Y and SK-N-SH cells, confirmed by a larger scale culture condition. Our data is the first in vitro evidence that fluoxetine could increase the expression of miRNAs in undifferentiated neural cells, and that putative target genes of those miRNAs have been shown to be involved in fundamental neurodevelopmental processes. PMID:27716787

  11. Use of Mature miRNA Strand Selection in miRNAs Families in Cervical Cancer Development

    Directory of Open Access Journals (Sweden)

    Angelica Judith Granados-López

    2017-02-01

    Full Text Available Aberrant miRNA expression is well recognized as a cancer hallmark, nevertheless miRNA function and expression does not always correlate in patients tissues and cell lines studies. In addition to this issue, miRNA strand usage conduces to increased cell signaling pathways modulation diversifying cellular processes regulation. In cervical cancer, 20 miRNA families are involved in carcinogenesis induction and development to this moment. These families have 5p and 3p strands with different nucleotide (nt chain sizes. In general, mature 5p strands are larger: two miRNAs of 24 nt, 24 miRNAs of 23 nt, 35 miRNAs of 22 nt and three miRNAs of 21 nt. On the other hand, the 3p strands lengths observed are: seven miRNAs of 23 nt, 50 miRNAs of 22 nt, six miRNAs of 21 nt and four miRNAs of 20 nt. Based on the analysis of the 20 miRNA families associated with cervical cancer, 67 3p strands and 65 5p strands are selected suggesting selectivity and specificity mechanisms regulating cell processes like proliferation, apoptosis, migration, invasion, metabolism and Warburg effect. The insight reviewed here could be used in the miRNA based therapy, diagnosis and prognosis approaches.

  12. Use of Mature miRNA Strand Selection in miRNAs Families in Cervical Cancer Development

    Science.gov (United States)

    Granados-López, Angelica Judith; Ruiz-Carrillo, José Luis; Servín-González, Luis Steven; Martínez-Rodríguez, José Luis; Reyes-Estrada, Claudia Araceli; Gutiérrez-Hernández, Rosalinda; López, Jesús Adrián

    2017-01-01

    Aberrant miRNA expression is well recognized as a cancer hallmark, nevertheless miRNA function and expression does not always correlate in patients tissues and cell lines studies. In addition to this issue, miRNA strand usage conduces to increased cell signaling pathways modulation diversifying cellular processes regulation. In cervical cancer, 20 miRNA families are involved in carcinogenesis induction and development to this moment. These families have 5p and 3p strands with different nucleotide (nt) chain sizes. In general, mature 5p strands are larger: two miRNAs of 24 nt, 24 miRNAs of 23 nt, 35 miRNAs of 22 nt and three miRNAs of 21 nt. On the other hand, the 3p strands lengths observed are: seven miRNAs of 23 nt, 50 miRNAs of 22 nt, six miRNAs of 21 nt and four miRNAs of 20 nt. Based on the analysis of the 20 miRNA families associated with cervical cancer, 67 3p strands and 65 5p strands are selected suggesting selectivity and specificity mechanisms regulating cell processes like proliferation, apoptosis, migration, invasion, metabolism and Warburg effect. The insight reviewed here could be used in the miRNA based therapy, diagnosis and prognosis approaches. PMID:28216603

  13. "Seed-Milarity" confers to hsa-miR-210 and hsa-miR-147b similar functional activity.

    Directory of Open Access Journals (Sweden)

    Thomas Bertero

    Full Text Available Specificity of interaction between a microRNA (miRNA and its targets crucially depends on the seed region located in its 5'-end. It is often implicitly considered that two miRNAs sharing the same biological activity should display similarity beyond the strict six nucleotide region that forms the seed, in order to form specific complexes with the same mRNA targets. We have found that expression of hsa-miR-147b and hsa-miR-210, though triggered by different stimuli (i.e. lipopolysaccharides and hypoxia, respectively, induce very similar cellular effects in term of proliferation, migration and apoptosis. Hsa-miR-147b only shares a "minimal" 6-nucleotides seed sequence with hsa-miR-210, but is identical with hsa-miR-147a over 20 nucleotides, except for one base located in the seed region. Phenotypic changes induced after heterologous expression of miR-147a strikingly differ from those induced by miR-147b or miR-210. In particular, miR-147a behaves as a potent inhibitor of cell proliferation and migration. These data fit well with the gene expression profiles observed for miR-147b and miR-210, which are very similar, and the gene expression profile of miR-147a, which is distinct from the two others. Bioinformatics analysis of all human miRNA sequences indicates multiple cases of miRNAs from distinct families exhibiting the same kind of similarity that would need to be further characterized in terms of putative functional redundancy. Besides, it implies that functional impact of some miRNAs can be masked by robust expression of miRNAs belonging to distinct families.

  14. A path-based measurement for human miRNA functional similarities using miRNA-disease associations

    Science.gov (United States)

    Ding, Pingjian; Luo, Jiawei; Xiao, Qiu; Chen, Xiangtao

    2016-09-01

    Compared with the sequence and expression similarity, miRNA functional similarity is so important for biology researches and many applications such as miRNA clustering, miRNA function prediction, miRNA synergism identification and disease miRNA prioritization. However, the existing methods always utilized the predicted miRNA target which has high false positive and false negative to calculate the miRNA functional similarity. Meanwhile, it is difficult to achieve high reliability of miRNA functional similarity with miRNA-disease associations. Therefore, it is increasingly needed to improve the measurement of miRNA functional similarity. In this study, we develop a novel path-based calculation method of miRNA functional similarity based on miRNA-disease associations, called MFSP. Compared with other methods, our method obtains higher average functional similarity of intra-family and intra-cluster selected groups. Meanwhile, the lower average functional similarity of inter-family and inter-cluster miRNA pair is obtained. In addition, the smaller p-value is achieved, while applying Wilcoxon rank-sum test and Kruskal-Wallis test to different miRNA groups. The relationship between miRNA functional similarity and other information sources is exhibited. Furthermore, the constructed miRNA functional network based on MFSP is a scale-free and small-world network. Moreover, the higher AUC for miRNA-disease prediction indicates the ability of MFSP uncovering miRNA functional similarity.

  15. Dynamic expression of miR-132, miR-212, and miR-146 in the brain of different hosts infected with Angiostrongylus cantonensis.

    Science.gov (United States)

    Yu, Liping; Liao, Qi; Chen, Xiaoguang; Xu, Lian; Zeng, Xin; Lv, Zhiyue; Sun, Xi; Zhen, Huanqin; Wu, Zhongdao

    2014-01-01

    Increasing evidence shows that microRNAs (miRNAs) are a family of regulatory molecules involved in many physiological processes, including the inflammation in central nervous system (CNS) and neurological disorders. Angiostrongylus cantonensis (A. cantonensis) is the major cause of human infectious eosinophilic meningitis and can induce CNS injury. In the present study, we investigated the expression of miRNAs involved in neuronal functions such as miR-132-3p/212-3p, and miR-146a-5p, inflammation-related miRNA, in the modulation of inflammation of CNS of mice and rats induced by A. cantonensis. The functions of differentially expressed miRNAs were analyzed through bioinformatics methods, and the expression of chosen target genes were investigated by quantitative reverse transcription polymerase chain reaction. The results showed that miR-146a-5p upregulated in the brain of rats after 21 days; A. cantonensis infection and the expression of miR-132-3p and miR-146a-5p upregulated in the brain of mice model infected by A. cantonensis. The expression of the target genes of mmu-miR-146a-5p such as Irak1 and Traf6 downregulated in 14 days and 21 days after A. cantonensis infection. Our results supply more information about the involvement of the miRNAs in the regulation of inflammation of CNS induced by A. cantonensis.

  16. EL CIRCO Y MI DILEMA

    Directory of Open Access Journals (Sweden)

    Martalucía Tamayo

    2015-03-01

    habilidades excepcionales, temo que lo que se esté buscando (¿o logrando? es un aplauso lleno de compasión o lástima por su condición de “seres diferentes”; o más bien agradados por haber presenciado “un fenómeno” digno de ser exhibido para la curiosidad morbosa de la gente. Ojalá no haya sido por eso. Tengo el mismo sentimiento de rechazo a los movimientos tipo “Teletón”, que suelen recoger ayuda económica mediante la explotación de un sentimiento de “compasión y lástima”. He luchado toda mi vida para que la sociedad no olvide el valor de la diferencia, el respeto a ella, el derecho a ser y vivir diferente; y hemos trabajado por muchos años para que esas personas “diferentes” sean (seamos “sujetos de derechos” igual que cualquier otro ser humano. Lo triste, y he ahí mi dilema, es que muchas personas del público comentan “pobrecitos, tan tiernos, al menos estos dos tienen empleo y viven de algo” y me digo que es verdad; son de los pocos que quizás logren sobrevivir económicamente bien en un mundo que está lejos de ser fácil para ellos. Pero ahí surge el dilema y el temor de que la sociedad aprenda a ver las cosas de manera equivocada. Y... ¿la dignidad? ¿Dónde queda la concientización o sensibilización de la sociedad? ¿Dónde queda el respeto a la diferencia? ¿A quién podría gustarle ser exhibido como fenómeno? ¿Alguien se ha preguntado el drama que vive el “ser diferente” en un mundo que no está diseñado para todo el mundo?...

  17. Dissection of miRNA pathways using arabidopsis mesophyll protoplasts.

    Science.gov (United States)

    Martinho, Cláudia; Confraria, Ana; Elias, Carlos Alexandre; Crozet, Pierre; Rubio-Somoza, Ignacio; Weigel, Detlef; Baena-González, Elena

    2015-02-01

    MicroRNAs (miRNAs) control gene expression mostly post-transcriptionally by guiding transcript cleavage and/or translational repression of complementary mRNA targets, thereby regulating developmental processes and stress responses. Despite the remarkable expansion of the field, the mechanisms underlying miRNA activity are not fully understood. In this article, we describe a transient expression system in Arabidopsis mesophyll protoplasts, which is highly amenable for the dissection of miRNA pathways. We show that by transiently overexpressing primary miRNAs and target mimics, we can manipulate miRNA levels and consequently impact on their targets. Furthermore, we developed a set of luciferase-based sensors for quantifying miRNA activity that respond specifically to both endogenous and overexpressed miRNAs and target mimics. We demonstrate that these miRNA sensors can be used to test the impact of putative components of the miRNA pathway on miRNA activity, as well as the impact of specific mutations, by either overexpression or the use of protoplasts from the corresponding mutants. We further show that our miRNA sensors can be used for investigating the effect of chemicals on miRNA activity. Our cell-based transient expression system is fast and easy to set up, and generates quantitative results, being a powerful tool for assaying miRNA activity in vivo.

  18. miEAA: microRNA enrichment analysis and annotation.

    Science.gov (United States)

    Backes, Christina; Khaleeq, Qurratulain T; Meese, Eckart; Keller, Andreas

    2016-07-08

    Similar to the development of gene set enrichment and gene regulatory network analysis tools over a decade ago, microRNA enrichment tools are currently gaining importance. Building on our experience with the gene set analysis toolkit GeneTrail, we implemented the miRNA Enrichment Analysis and Annotation tool (miEAA). MiEAA is a web-based application that offers a variety of commonly applied statistical tests such as over-representation analysis and miRNA set enrichment analysis, which is similar to Gene Set Enrichment Analysis. Besides the different statistical tests, miEAA also provides rich functionality in terms of miRNA categories. Altogether, over 14 000 miRNA sets have been added, including pathways, diseases, organs and target genes. Importantly, our tool can be applied for miRNA precursors as well as mature miRNAs. To make the tool as useful as possible we additionally implemented supporting tools such as converters between different miRBase versions and converters from miRNA names to precursor names. We evaluated the performance of miEAA on two sets of miRNAs that are affected in lung adenocarcinomas and have been detected by array analysis. The web-based application is freely accessible at: http://www.ccb.uni-saarland.de/mieaa_tool/.

  19. miRNA expression during prickly pear cactus fruit development.

    Science.gov (United States)

    Rosas-Cárdenas, Flor de Fátima; Caballero-Pérez, Juan; Gutiérrez-Ramos, Ximena; Marsch-Martínez, Nayelli; Cruz-Hernández, Andrés; de Folter, Stefan

    2015-02-01

    miRNAs are a class of small non-coding RNAs that regulate gene expression. They are involved in the control of many developmental processes, including fruit development. The increasing amount of information on miRNAs, on their expression, abundance, and conservation between various species, provides a new opportunity to study the role of miRNAs in non-model plant species. In this work, we used a combination of Northern blot and tissue print hybridization analysis to identify conserved miRNAs expressed during prickly pear cactus (Opuntia ficus indica) fruit development. Comparative profiling detected the expression of 34 miRNAs, which were clustered in three different groups that were associated with the different phases of fruit development. Variation in the level of miRNA expression was observed. Gradual expression increase of several miRNAs was observed during fruit development, including miR164. miR164 was selected for stem-loop RT-PCR and for a detailed spatial-temporal expression analysis. At early floral stages, miR164 was mainly localized in meristematic tissues, boundaries and fusion zones, while it was more homogenously expressed in fruit tissues. Our results provide the first evidence of miRNA expression in the prickly pear cactus and provide the basis for future research on miRNAs in Opuntia. Moreover, our analyses suggest that miR164 plays different roles during prickly pear cactus fruit development.

  20. Final Report for the Arroyo Las Positas Maintenance Impact Study, Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    van Hattem, M; Paterson, L

    2006-01-12

    In 2000, the Lawrence Livermore National Laboratory's (LLNL) Environmental Protection Department, in coordination with Plant Engineering (PE), began dredging sections of the Arroyo Las Positas (ALP) to alleviate concerns about flooding of sensitive facilities within the mainsite of Lawrence Livermore National Laboratory. In order to reduce potential impacts on the federally threatened California red-legged frog (Rana aurora draytonii), LLNL proposed to dredge sections of the ALP in a ''checkerboard pattern'', resulting in a mosaic of open water habitat and vegetated sections (Figure 1). The Arroyo Las Positas Management Plan (Plan) was coordinated with both state and federal agencies including the U.S. Fish and Wildlife Service (USFWS), California Department of Fish and Game (CDF&G), San Francisco Regional Water Quality Control Board (SFRWQCB), and the Army Corp of Engineers (ACOE). Water Discharge Requirements (WDRs) were issued for this project on December 30, 1999 (Order No. 99-086) by the SFRWQCB. Provision 19 of the WDRs outlined a five-year (2000 through 2004) Maintenance Impact Study (MIS) that LLNL began in coordination with dredging work that was conducted as part of the Arroyo Las Positas Management Plan. Provision 20 of these WDRs requires LLNL to submit a final report of the results of the Maintenance Impact Study for this project to the SFRWQCB. The purpose of this report is to present the results of the Maintenance Impact Study for Arroyo Las Positas and meet the requirements of Provision 20. A description of the annual monitoring included in this Maintenance Impact Study is included in the methods section of this report. Initially the Plan called for dredging the entire length of the Arroyo Las Positas (approximately 6,981 linear feet) over a 5-year period to minimize temporal impacts on the California red-legged frog. Dredging occurred in 2000 ({approx}1,300 ft.), 2001 ({approx}800 ft.), and 2002 ({approx}1,200 ft.), which

  1. Differential Expression of miR-21, miR-126, miR-143, miR-373 in Normal Cervical Tissue, Cervical Cancer Tissue and Hela Cell%miR-21、miR-126、miR-143和miR-373在正常宫颈组织、宫颈癌组织及Hela细胞中的表达差异

    Institute of Scientific and Technical Information of China (English)

    刘琳; 王月玲; 王江芬

    2012-01-01

    目的 研究miR-21、miR-126、miR-143和miR-373在正常宫颈组织、宫颈癌组织及Hela细胞中的表达差异,探讨microRNAs(miRNAs)对宫颈癌发生的调控作用.方法 荧光实时定量检测20例良性肿瘤患者的正常宫颈组织,20例宫颈癌组织及Hela细胞中miR-21、miR-126、miR-143和miR-373的表达.结果 m iR-21在宫颈癌组织及Hela细胞系中高表达,在正常宫颈标本中低表达,在宫颈癌组织中相对定量为正常宫颈组织的11.3196倍(P<0.05);miR-143、miR-373在宫颈癌组织及Hela细胞中均低表达,在正常宫颈标本中高表达,miR-143、miR-373在宫颈癌组织中相对定量分别为正常宫颈组织的0.1553倍和0.4907倍(P<0.05);miR-126的表达无明显变化.结论 miRNAs与宫颈癌的发生和调控密切相关.在宫颈癌组织和Hela细胞中,miR-21表达上调,可能在宫颈癌的发生过程中发挥癌基因的作用;miR-143、miR-373表达下调可能发挥抑癌基因的作用;miR-126表达无差异,与宫颈癌无明显关系.%Objective To investigate the differential expression of miR-21 ,miR-126 ,miR-143 and miR-373 in normal cervical tissue, cervical cancer tissue and Hela cell.Methods The expressions of miR-21, miR-126, miR-143 and miR-373 were detected by real-time PCR in cervical cancer tissue,cervical tissue of benign uterine tumor and Hela cell. Results High expression of miR-21 was observed in cervical cancer and Hela cell, while low expression was observed in normal cervical tissue. The relative quantification of miR-21 in cerveical cancer was 11.3196 times that of miR-21 in normal cervical tissue (P0. 05). Conclusion miRNAs are closely related to the occurrence and regulation of cervical cancer. The high expression of miR-21 in cervical cancer and Hela cell indicate that it may play a possible role of oncogenes, while miR-143 and miR-373 with low expression may play the role of tumor suppressor genes.

  2. 淋巴瘤患者血浆中miR-21、miR-155、miR-210的表达及临床意义%The expression and clinical significance of miR-21,miR-155,miR-210 of plasma lymphoma patients

    Institute of Scientific and Technical Information of China (English)

    曹慧琴; 韦玮

    2016-01-01

    目的:探讨 miR-21、miR-155、miR-210的临床表达意义及其与淋巴瘤病理特征的相关性。方法采用RT-PCR 技术及免疫组化法测定60例行淋巴瘤患者血清中 miR-21、miR-155、miR-210表达量及 BCL-6蛋白水平,并对其与淋巴瘤病理特征相关性进行分析。结果与正常对照组相比,淋巴瘤患者 miR-21、miR-155、miR-210表达量显著提高,差异有统计学意义(P <0.05)。经相关分析可知,miR-21、miR-155表达量与免疫分型、MYC 基因、BCL-6蛋白有关,而 miR-210表达量则与免疫分型、MYC 基因有关。结论miR-21、miR-155、miR-210可能与淋巴瘤发生及进展相关。通过测定 miR-21、miR-155、miR-210表达情况可有效预测淋巴瘤病情的进展,对临床淋巴瘤诊治具有重要的指导意义。%Objective To investigate the expression and clinical significance of miR-21,miR-155,miR-210 of plasma lymphoma patients.Methods The expression levels of miR-21,miR-155,miR-210 and BCL-6 protein of 60 cases of plasma lymphoma patients were measured with RT-PCR and immunohistochemical staining,the features associated with lymphoma were analyzed.Results The levels of miR-21,miR-155,miR-210 of plasma lymphoma patients were higher than control group(P <0.05).The expression of miR-21,miR-155 were relationship with immune typing,MYC gene, BCL-6 protein,whereas the expression of miR-210 were relationship with immune typing,MYC genes.Conclusion miR-21,miR-155,miR-210 may occur and progress associated with lymphoma.By measuring miR-21,miR-155,miR-210 expression can predict disease progression of lymphoma,has important significance for clinical diagnosis and treatment of lymphoma.

  3. Allelic and haplotype variation of major histocompatibility complex class II DRB1 and DQB loci in the St Lawrence beluga (Delphinapterus leucas).

    Science.gov (United States)

    1999-07-01

    In order to assess levels of major histocompatibility complex (Mhc) variation within the St Lawrence beluga (Delphinapterus leucas) the variation at the beluga Mhc DRB1 class II locus was assessed by single-strand conformation polymorphism (SSCP) analysis of the peptide-binding region for 313 whales collected from 13 sampling locations across North America. In addition, samples from west Greenland and the St Lawrence were also typed at the DQB locus, allowing comparison to a previous study and assessment of linkage disequilibrium of alleles at the two loci. Comparisons of DRB1 and DQB allele frequencies among all sampling locations indicated genetic structure (alpha St Lawrence, Hudson Strait, Bering Sea, Cunningham Inlet, and Davis Strait (minus Cunningham Inlet), except the St Lawrence and Hudson Strait for the DQB locus. In the St Lawrence population, six of the eight DRB1 alleles are present representing all five known allelic lineages. Evidence of linkage disequilibrium between the DRB1 and DQB is present in two sampling locations, the St Lawrence and Nuussuaq (alpha = 0.05). Analysis of probable DRB1-DQB haplotypes among groups of beluga suggests a haplotype reduction in the St Lawrence.

  4. Inhibition of 14q32 MicroRNAs miR-329, miR-487b, miR-494, and miR-495 Increases Neovascularization and Blood Flow Recovery After Ischemia

    DEFF Research Database (Denmark)

    Welten, S. M. J.; Bastiaansen, Ajnm; de Jong, R. C. M.;

    2014-01-01

    in mice after single femoral artery ligation. Methods and Results: Gene silencing oligonucleotides (GSOs) were used to inhibit 4 14q32 microRNAs, miR-329, miR-487b, miR-494, and miR-495, 1 day before double femoral artery ligation. Blood flow recovery was followed by laser Doppler perfusion imaging. All 4......, as well as to increased arterial myofibroblast proliferation. Conclusions: The 14q32 microRNA gene cluster is highly involved in neovascularization. Inhibition of 14q32 microRNAs miR-329, miR-487b, miR-494, and miR-495 provides a promising tool for future therapeutic neovascularization....

  5. Expression of miR-143 and miR-145 and their functional study in gastric carcinoma%miR-143和miR-145在胃癌中的表达及功能研究

    Institute of Scientific and Technical Information of China (English)

    邢晓芳; 李子禹

    2015-01-01

    Objective To investigate the expressions of miR-143 and miR-145 in gastric cancer tissues and their biological functions. Methods A set of gastric cancer metastasis-related miRNAs was screened by comparing the miRNA profiles between the primary gastric cancer and matched liver metastasis. Among these miRNAs, expressions of miR-143 and miR-145 were further validated. The influence on metastasis was analyzed by transwell assay. Results Expressions of miR-143 and miR-145 down-regulated significantly in gastric cancer tissues compared to adjacent normal tissues (miR-143, 0.028 ±0.005 vs. 0.052 ±0.014, P=0.058; miR-145, 0.922 ±0.135 vs. 1.722 ±0.285, P=0.007), and decreased significantly in the metastatic lesion compared with the primary lesion (miR-143, 0.059±0.025 vs. 0.182±0.045, P=0.021; miR-145, 0.164±0.076 vs. 0.594±0.283, P=0.042). Expressions of miR-143 and miR-145 were significantly correlated (r=0.400, P=0.000), and they synergistically inhibited gastric cancer cell migration. Conclusion miR-143 and miR-145 may take part in the progression of gastric cancer metastasis, and they may have synergetic effects.%目的:探讨miR-143和miR-145在胃癌组织中的表达情况,观察其生物学功能。方法通过高通量芯片检测比较11对胃癌原发灶及其配对肝转移灶的miRNA表达谱,采用Real-time PCR方法观察miR-145和miR-143在55例胃癌组织中的表达情况。同时使用Transwell方法观察其对细胞转移能力的影响。结果 miR-143和miR-145在胃癌组织中的表达明显低于癌旁正常组织(miR-143:0.028±0.005比0.052±0.014,P=0.058;miR-145:0.922±0.135比1.772±0.285,P=0.007),在转移灶中的表达明显低于原发灶(miR-143:0.059±0.025比0.182±0.045,P=0.021;miR-145:0.164±0.076比0.594±0.283,P=0.042),相关性分析显示,miR-143和miR-145表达具有显著相关性(r=0.400, P=0.000)。体外实验显

  6. Circular RNA and miR-7 in Cancer

    DEFF Research Database (Denmark)

    Hansen, Thomas Birkballe; Kjems, Jørgen; Damgaard, Christian Kroun

    2013-01-01

    MicroRNAs (miRNA) play important roles in fine-tuning gene expression and are often deregulated in cancer. The identification of competing endogenous RNA and circular RNA (circRNA) as important regulators of miRNA activity underscores the increasing complexity of ncRNA-mediated regulatory networks....... Particularly, the recently identified circular RNA, ciRS-7, which acts as a designated miR-7 inhibitor/sponge, has conceptually changed the mechanistic understanding of miRNA networks. As miR-7 modulates the expression of several oncogenes, disclosing the regulation of miR-7 activity will likely advance...... the understanding of various cancer etiologies. Here, we review the current knowledge about the ciRS-7/miR-7 axis in cancer-related pathways and discuss possible models explaining the relevance of coexpressing miR-7 along with a circRNA inhibitor....

  7. miR-24 and miR-205 expression is dependent on HPV onco-protein expression in keratinocytes

    Energy Technology Data Exchange (ETDEWEB)

    McKenna, Declan J., E-mail: dj.mckenna@ulster.ac.uk [Biomedical Sciences Research Institute, University of Ulster, Coleraine, Co. Derry BT52 1SA (United Kingdom); Centre for Cancer Research and Cell Biology, School of Medicine, Dentistry and Biomedical Science, Queen' s University Belfast, Belfast BT9 7BL (United Kingdom); Patel, Daksha, E-mail: d.patel@qub.ac.uk [Centre for Cancer Research and Cell Biology, School of Medicine, Dentistry and Biomedical Science, Queen' s University Belfast, Belfast BT9 7BL (United Kingdom); McCance, Dennis J., E-mail: d.mccance@qub.ac.uk [Centre for Cancer Research and Cell Biology, School of Medicine, Dentistry and Biomedical Science, Queen' s University Belfast, Belfast BT9 7BL (United Kingdom)

    2014-01-05

    A screen of microRNA (miRNA) expression following differentiation in human foreskin keratinocytes (HFKs) identified changes in several miRNAs, including miR-24 and miR-205. We investigated how expression of Human Papilloma Virus Type-16 (HPV16) onco-proteins E6 and E7 affected expression of miR-24 and miR-205 during proliferation and differentiation of HFKs. We show that the induction of both miR-24 and miR-205 observed during differentiation of HFKs is lost in HFKs expressing E6 and E7. We demonstrate that the effect on miR-205 is due to E7 activity, as miR-205 expression is dependent on pRb expression. Finally, we provide evidence that miR-24 effects in the cell may be due to targeting of cyclin dependent kinase inhibitor p27. In summary, these results indicate that expression of both miR-24 and miR-205 are impacted by E6 and/or E7 expression, which may be one mechanism by which HPV onco-proteins can disrupt the balance between proliferation and differentiation in keratinocytes. - Highlights: • miR-24 and miR-205 are induced during keratinocyte differentiation. • This induction is lost in keratinocytes expressing HPV onco-proteins E6 and E7. • miR-205 is dependent upon pRb expression. • miR-24 targets p27 in cycling keratinocytes.

  8. Overexpression of miR-484 and miR-744 in Vero cells alters Dengue virus replication

    Science.gov (United States)

    Castrillón-Betancur, Juan Camilo; Urcuqui-Inchima, Silvio

    2017-01-01

    BACKGROUND Dengue is considered one of the world’s most important mosquito-borne diseases. MicroRNAs (miRNAs) are small non-coding single-stranded RNAs that play an important role in the regulation of gene expression in eukaryotes. Although miRNAs possess antiviral activity against many mammalian-infecting viruses, their involvement in Dengue virus (DENV) replication remains poorly understood. OBJECTIVE To determine the role of miR-484 and miR-744 in DENV infection and to examine whether DENV infection alters the expression of both miRNAs. METHODS We used bioinformatics tools to explore the relationship between DENV and cellular miRNAs. We then overexpressed miR-484 or miR-744 in Vero cells to examine their role in DENV replication using flow cytometry, reverse transcriptase quantitative polymerase chain reaction (RT-qPCR), and western blotting. FINDINGS We found several cellular miRNAs that target a conserved region within the 3′ untranslated region (3′ UTR) of the genome of the four DENV serotypes and found that overexpression of miR-484 or miR-744 inhibits infection by DENV-1 to DENV-4. Furthermore, we observed that DENV RNA might be involved in the downregulation of endogenous miR-484 and miR-744. CONCLUSION Our study identifies miR-484 and miR-744 as two possible restriction host factors against DENV infection. However, further studies are needed to directly verify whether miR-484 and miR-744 both have an anti-DENV effect in vivo. PMID:28327787

  9. A miRNA-responsive cell-free translation system facilitates isolation of hepatitis C virus miRNP complexes

    Science.gov (United States)

    Bradrick, Shelton S.; Nagyal, Simardeep; Novatt, Hilary

    2013-01-01

    Micro(mi)RNAs are 21- to 23-nt RNAs that regulate multiple biological processes. In association with Argonaute (Ago) proteins and other factors that form the RNA-induced silencing complex (RISC), miRNAs typically bind mRNA 3′ untranslated regions (UTRs) and repress protein production through antagonizing translation and transcript stability. For a given mRNA–miRNA interaction, cis-acting RNA elements and trans-acting RNA-binding proteins (RBPs) may influence mRNA fate. This is particularly true of the hepatitis C virus (HCV) genome which interacts with miR-122, an abundant liver miRNA. miR-122 binding to HCV RNA considerably stimulates virus replication in cultured cells and primates, but the mechanism(s) and associated host factors required for enhancement of HCV replication have not been fully elucidated. We recapitulated miR-122–HCV RNA interactions in a cell-free translation system derived from cells that express miR-122. Specifically, lysates produced from HEK-293 cells that inducibly transcribe and process pri-miR-122 were characterized alongside those from isogenic cells lacking miR-122 expression. We observed a stimulatory effect of miR-122 on HCV reporter mRNAs in a manner that depended on expression of miR-122 and intact target sites within the HCV 5′ UTR. We took advantage of this system to affinity-purify miR-122-HCV RNP complexes. Similar to functional assays, we found that association of immobilized HCV internal ribosome entry site (IRES) RNA with endogenous Ago2 requires both miR-122 expression and intact miR-122 target sites in cis. This combined approach may be generalizable to affinity purification of miRNP complexes for selected target mRNAs, allowing identification of miRNP components and RBPs that may contribute to regulation. PMID:23793894

  10. miR-132 and miR-212 are increased in pancreatic cancer and target the retinoblastoma tumor suppressor.

    Science.gov (United States)

    Park, Jong-Kook; Henry, Jon C; Jiang, Jinmai; Esau, Christine; Gusev, Yuriy; Lerner, Megan R; Postier, Russell G; Brackett, Daniel J; Schmittgen, Thomas D

    2011-03-25

    Numerous microRNAs (miRNAs) are reported as differentially expressed in cancer, however the consequence of miRNA deregulation in cancer is unknown for many miRNAs. We report that two miRNAs located on chromosome 17p13, miR-132 and miR-212, are over-expressed in pancreatic adenocarcinoma (PDAC) tissues. Both miRNAs are predicted to target the retinoblastoma tumor suppressor, Rb1. Validation of this interaction was confirmed by luciferase reporter assay and western blot in a pancreatic cancer cell line transfected with pre-miR-212 and pre-miR-132 oligos. Cell proliferation was enhanced in Panc-1 cells transfected with pre-miR-132/-212 oligos. Conversely, antisense oligos to miR-132/-212 reduced cell proliferation and caused a G(2)/M cell cycle arrest. The mRNA of a number of E2F transcriptional targets were increased in cells over expressing miR-132/-212. Exposing Panc-1 cells to the β2 adrenergic receptor agonist, terbutaline, increased the miR-132 and miR-212 expression by 2- to 4-fold. We report that over-expression of miR-132 and miR-212 result in reduced pRb protein in pancreatic cancer cells and that the increase in cell proliferation from over-expression of these miRNAs is likely due to increased expression of several E2F target genes. The β2 adrenergic pathway may play an important role in this novel mechanism. Copyright © 2011 Elsevier Inc. All rights reserved.

  11. Real-time PCR in detection of plasmic miRNA in patients with esophageal cancer%实时荧光定量PCR检测食管癌循环miRNA的研究

    Institute of Scientific and Technical Information of China (English)

    彭学宏; 陈暖; 李克; 郑邦希

    2014-01-01

    Objective To investigate the differential expression of miR-424,miR-373,miR-34c,miR-129,miR-130b,miR-203,miR-9,miR-15b,miR-100,miR-145,miR-21 in serum of esophageal cancer.Methods The expression levels of miR-424,miR-373,miR-34c,miR-129,miR-130b,miR-203,miR-9,miR-15b,miR-100,miR-145,miR-21 were detected by real-time PCR in serum of 20 human esophageal cancer and 20 normal person.Results Over-expression of miR-424,miR-373,miR-34c,miR-203,miR-15b and low-expression of miR-100 were observed in serum of esophageal cancer.Conclusions miRNAs in the serum maybe a new biological indicator for diagnosis of human esophageal cancer.%目的 研究miR-424、miR-373、miR-34c、miR-129、miR-130b、miR-203、miR-9、miR-15b、miR-100、miR-145、miR-21在食管癌患者外周血的差异表达.方法 采用Real-time PCR定量检测方法分析比较20例食管癌患者血液与健康对照者血液之间miR-424、miR-373、miR-34c、miR-129、miR-130b、miR-203、miR-9、miR-15b、miR-100、miR-145、miR-21表达差异情况.结果 miR-424、miR-373、miR-34c、miR-203、miR-15b表达上调,miR-100表达下调.结论 外周血miRNA可能成为食管癌患者早期诊断新的生物学指标.

  12. miR-132 and miR-212 are increased in pancreatic cancer and target the retinoblastoma tumor suppressor

    Energy Technology Data Exchange (ETDEWEB)

    Park, Jong-Kook [College of Pharmacy, Ohio State University, Columbus, OH 43210 (United States); Henry, Jon C. [Department of Surgery, Ohio State University, Columbus, OH 43210 (United States); Jiang, Jinmai [College of Pharmacy, Ohio State University, Columbus, OH 43210 (United States); Esau, Christine [Regulus Therapeutics, Carlsbad, CA (United States); Gusev, Yuriy [Lombardi Cancer Center, Georgetown University, Washington, DC (United States); Lerner, Megan R. [Veterans Affairs Medical Center, Oklahoma City, OK (United States); Postier, Russell G. [Department of Surgery, University of Oklahoma Health Sciences Center, Oklahoma City, OK (United States); Brackett, Daniel J. [Veterans Affairs Medical Center, Oklahoma City, OK (United States); Schmittgen, Thomas D., E-mail: Schmittgen.2@osu.edu [College of Pharmacy, Ohio State University, Columbus, OH 43210 (United States)

    2011-03-25

    Research highlights: {yields} The expression of miR-132 and miR-212 are significantly increased in pancreatic cancer. {yields} miR-132 and miR-212 target the tumor suppressor pRb, resulting in enhanced proliferation. {yields} miR-132 and miR-212 expression is increased by a {beta}2 adrenergic receptor agonist, suggesting a novel mechanism for pancreatic cancer progression. -- Abstract: Numerous microRNAs (miRNAs) are reported as differentially expressed in cancer, however the consequence of miRNA deregulation in cancer is unknown for many miRNAs. We report that two miRNAs located on chromosome 17p13, miR-132 and miR-212, are over-expressed in pancreatic adenocarcinoma (PDAC) tissues. Both miRNAs are predicted to target the retinoblastoma tumor suppressor, Rb1. Validation of this interaction was confirmed by luciferase reporter assay and western blot in a pancreatic cancer cell line transfected with pre-miR-212 and pre-miR-132 oligos. Cell proliferation was enhanced in Panc-1 cells transfected with pre-miR-132/-212 oligos. Conversely, antisense oligos to miR-132/-212 reduced cell proliferation and caused a G{sub 2}/M cell cycle arrest. The mRNA of a number of E2F transcriptional targets were increased in cells over expressing miR-132/-212. Exposing Panc-1 cells to the {beta}2 adrenergic receptor agonist, terbutaline, increased the miR-132 and miR-212 expression by 2- to 4-fold. We report that over-expression of miR-132 and miR-212 result in reduced pRb protein in pancreatic cancer cells and that the increase in cell proliferation from over-expression of these miRNAs is likely due to increased expression of several E2F target genes. The {beta}2 adrenergic pathway may play an important role in this novel mechanism.

  13. Exosomes as miRNA Carriers: Formation-Function-Future.

    Science.gov (United States)

    Yu, Xiaojie; Odenthal, Margarete; Fries, Jochen W U

    2016-12-02

    Exosomes, which are one of the smallest extracellular vesicles released from cells, have been shown to carry different nucleic acids, including microRNAs (miRNAs). miRNAs significantly regulate cell growth and metabolism by posttranscriptional inhibition of gene expression. The rapidly changing understanding of exosomes' formation and function in delivering miRNAs from cell to cell has prompted us to review current knowledge in exosomal miRNA secretion mechanisms as well as possible therapeutic applications for personalized medicine.

  14. Construction quality assurance for Pit 6 landfill closure, Lawrence Livermore National Laboratory, Site 300

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-10-30

    Golder Construction Services, Inc. (GCS), under contract to the Regents of the University of California, Lawrence Livermore National Laboratory (LLNL), provided the construction quality assurance (CQA) observation and testing during the construction of the Site 300, Pit 6 landfill closure cover. The cap construction was performed as a CERCLA non-time-critical removal action from June 2 to August 29, 1997. the project site is located 18 miles east of Livermore on Tesla Road and approximately 10 miles southwest of Tracy on Corral Hollow Road in San Joaquin County, California. This report certifies that the LLNL, Site 300, Pit 6, Landfill Closure was constructed in accordance with the construction specifications and design drawings. This report documents construction activities and CQA monitoring and testing for construction of the Pit 6 Landfill Closure. Golder Associates, Inc. of Oakland, California was the design engineering firm responsible for preparation of the drawings and specifications. CQA services were provided by GCS, of Roseville, California, under supervision of a California registered civil Engineer.

  15. Screening Program Reduced Melanoma Mortality at the Lawrence Livermore National Laboratory, 1984-1996

    Energy Technology Data Exchange (ETDEWEB)

    Schneider, MD, J S; II, PhD, D; MD, PhD, M

    2006-10-12

    Worldwide incidence of cutaneous malignant melanoma has increased substantially, and no screening program has yet demonstrated reduction in mortality. We evaluated the education, self examination and targeted screening campaign at the Lawrence Livermore National Laboratory (LLNL) from its beginning in July 1984 through 1996. The thickness and crude incidence of melanoma from the years before the campaign were compared to those obtained during the 13 years of screening. Melanoma mortality during the 13-year period was based on a National Death Index search. Expected yearly deaths from melanoma among LLNL employees were calculated by using California mortality data matched by age, sex, and race/ethnicity and adjusted to exclude deaths from melanoma diagnosed before the program began or before employment at LLNL. After the program began, crude incidence of melanoma thicker than 0.75 mm decreased from 18 to 4 cases per 100,000 person-years (p = 0.02), while melanoma less than 0.75mm remained stable and in situ melanoma increased substantially. No eligible melanoma deaths occurred among LLNL employees during the screening period compared with a calculated 3.39 expected deaths (p = 0.034). Education, self examination and selective screening for melanoma at LLNL significantly decreased incidence of melanoma thicker than 0.75 mm and reduced the melanoma-related mortality rate to zero. This significant decrease in mortality rate persisted for at least 3 yr after employees retired or otherwise left the laboratory.

  16. Summary Report of Summer 2009 NGSI Human Capital Development Efforts at Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Dougan, A; Dreicer, M; Essner, J; Gaffney, A; Reed, J; Williams, R

    2009-11-16

    In 2009, Lawrence Livermore National Laboratory (LLNL) engaged in several activities to support NA-24's Next Generation Safeguards Initiative (NGSI). This report outlines LLNL's efforts to support Human Capital Development (HCD), one of five key components of NGSI managed by Dunbar Lockwood in the Office of International Regimes and Agreements (NA-243). There were five main LLNL summer safeguards HCD efforts sponsored by NGSI: (1) A joint Monterey Institute of International Studies/Center for Nonproliferation Studies-LLNL International Safeguards Policy and Information Analysis Course; (2) A Summer Safeguards Policy Internship Program at LLNL; (3) A Training in Environmental Sample Analysis for IAEA Safeguards Internship; (4) Safeguards Technology Internships; and (5) A joint LLNL-INL Summer Safeguards Lecture Series. In this report, we provide an overview of these five initiatives, an analysis of lessons learned, an update on the NGSI FY09 post-doc, and an update on students who participated in previous NGSI-sponsored LLNL safeguards HCD efforts.

  17. Ernest Orlando Lawrence Berkeley National Laboratory Institutional Plan FY 2000-2004

    Energy Technology Data Exchange (ETDEWEB)

    Chartock, Mike (ed.); Hansen, Todd (ed.)

    1999-08-01

    The FY 2000-2004 Institutional Plan provides an overview of the Ernest Orlando Lawrence Berkeley National Laboratory (Berkeley Lab, the Laboratory) mission, strategic plan, initiatives, and the resources required to fulfill its role in support of national needs in fundamental science and technology, energy resources, and environmental quality. To advance the Department of Energy's ongoing efforts to define the Integrated Laboratory System, the Berkeley Lab Institutional Plan reflects the strategic elements of our planning efforts. The Institutional Plan is a management report that supports the Department of Energy's mission and programs and is an element of the Department of Energy's strategic management planning activities, developed through an annual planning process. The Plan supports the Government Performance and Results Act of 1993 and complements the performance-based contract between the Department of Energy and the Regents of the University of California. It identifies technical and administrative directions in the context of the national energy policy and research needs and the Department of Energy's program planning initiatives. Preparation of the plan is coordinated by the Office of Planning and Communications from information contributed by Berkeley Lab's scientific and support divisions.

  18. Special-Status Plant Species Surveys and Vegetation Mapping at Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Preston, R E

    2006-10-03

    This report presents the results of Jones & Stokes special-status plant surveys and vegetation mapping for the University of California, Lawrence Livermore National Laboratory (LLNL). Special-status plant surveys were conducted at Site 300 in April to May 1997 and in March to April 2002. Eight special-status plants were identified at Site 300: large-flowered fiddleneck, big tarplant, diamond-petaled poppy, round-leaved filaree, gypsum-loving larkspur, California androsace, stinkbells, and hogwallow starfish. Maps identifying the locations of these species, a discussion of the occurrence of these species at Site 300, and a checklist of the flora of Site 300 are presented. A reconnaissance survey of the LLNL Livermore Site was conducted in June 2002. This survey concluded that no special-status plants occur at the Livermore Site. Vegetation mapping was conducted in 2001 at Site 300 to update a previous vegetation study done in 1986. The purpose of the vegetation mapping was to update and to delineate more precisely the boundaries between vegetation types and to map vegetation types that previously were not mapped. The vegetation map is presented with a discussion of the vegetation classification used.

  19. Delineation of Waters of the United States for Lawrence Livermore National Laboratory, Site 300

    Energy Technology Data Exchange (ETDEWEB)

    Preston, R E

    2006-09-25

    This report presents the results of a delineation of waters of the United States, including wetlands, for Lawrence Livermore National Laboratory's Site 300 in Alameda and San Joaquin Counties, California. Jones & Stokes mapped vegetation at Site 300 in August, 2001, using Global Positioning System (GPS) data recorders to collect point locations and to record linear features and map unit polygons. We identified wetlands boundaries in the field on the basis of the plant community present. We returned to collect additional information on wetland soils on July 3, 2002. Forty-six wetlands were identified, with a total area of 3.482 hectares (8.605 acres). The wetlands include vernal pools, freshwater seeps, and seasonal ponds. Wetlands appearing to meet the criteria for federal jurisdictional total 1.776 hectares (4.388 acres). A delineation map is presented and a table is provided with information on the type, size, characteristic plant species of each wetland, and a preliminary jurisdictional assessment.

  20. Environmental factors as predictors of epibenthic assemblage biomass in the St. Lawrence system

    Science.gov (United States)

    Bourget, E.; Ardisson, P.-L.; Lapointe, L.; Daigle, G.

    2003-07-01

    The distribution of epibenthic invertebrate biomass in relation to environmental factors was examined in the St. Lawrence system. Biomass estimates for epibenthos sampled yearly for 9 years on 102 suspended collectors (navigation buoys), were related to environmental data from the literature (surface water temperature, water salinity, water transparency, current velocity, chlorophyll a and primary production) using a weighted multiple linear regression analysis. Regression models were generated for total biomass and the biomass of the single dominant sessile species: Mytilus edulis, Semibalanus balanoides, Balanus crenatus, Obelia longissima and Hiatella arctica. Water temperature and water transparency, as well as some biogeographic groups of buoys represented by dummy variables, collectively explained 90.6% of the variance in total biomass. Water temperature, water transparency, biogeographic groups and, to a lesser degree, primary production, were the variables having a significant influence on the biomass of individual species. The lognormal weighted multiple regression model explained up to 84.5% of the variance in M. edulis biomass data and 67.9, 70.0, 71.6 and 38.9%, respectively, of the variance in S. balanoides, O. longissima, B. crenatus and H. arctica biomass data. The need to consider simultaneous biological and environmental sampling at the relevant temporal and spatial scales to model large marine coastal ecosystems is discussed.

  1. Ernest Orlando Lawrence Berkeley National Laboratory institutional plan, FY 1996--2001

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-11-01

    The FY 1996--2001 Institutional Plan provides an overview of the Ernest Orlando Lawrence Berkeley National Laboratory mission, strategic plan, core business areas, critical success factors, and the resource requirements to fulfill its mission in support of national needs in fundamental science and technology, energy resources, and environmental quality. The Laboratory Strategic Plan section identifies long-range conditions that will influence the Laboratory, as well as potential research trends and management implications. The Core Business Areas section identifies those initiatives that are potential new research programs representing major long-term opportunities for the Laboratory, and the resources required for their implementation. It also summarizes current programs and potential changes in research program activity, science and technology partnerships, and university and science education. The Critical Success Factors section reviews human resources; work force diversity; environment, safety, and health programs; management practices; site and facility needs; and communications and trust. The Resource Projections are estimates of required budgetary authority for the Laboratory`s ongoing research programs. The Institutional Plan is a management report for integration with the Department of Energy`s strategic planning activities, developed through an annual planning process. The plan identifies technical and administrative directions in the context of the national energy policy and research needs and the Department of Energy`s program planning initiatives. Preparation of the plan is coordinated by the Office of Planning and Communications from information contributed by the Laboratory`s scientific and support divisions.

  2. Fire History of Appalachian Forests of the Lower St-Lawrence Region (Southern Quebec

    Directory of Open Access Journals (Sweden)

    Serge Payette

    2017-04-01

    Full Text Available Sugar maple (Acer saccharum forests are among the main forest types of eastern North America. Sugar maple stands growing on Appalachian soils of the Lower St-Lawrence region are located at the northeastern limit of the northern hardwood forest zone. Given the biogeographical position of these forests at the edge of the boreal biome, we aimed to reconstruct the fire history and document the occurrence of temperate and boreal trees in sugar maple sites during the Holocene based on soil macrocharcoal analysis. Despite having experienced a different number of fire events, the fire history of the maple sites was broadly similar, with two main periods of fire activity, i.e., early- to mid-Holocene and late-Holocene. A long fire-free interval of at least 3500 years separated the two periods from the mid-Holocene to 2000 years ago. The maple sites differ with respect to fire frequency and synchronicity of the last millennia. According to the botanical composition of charcoal, forest vegetation remained relatively homogenous during the Holocene, except recently. Conifer and broadleaf species coexisted in mixed forests during the Holocene, in phase with fire events promoting the regeneration of boreal and temperate tree assemblages including balsam fir (Abies balsamea and sugar maple.

  3. Site safety plan for Lawrence Livermore National Laboratory CERCLA investigations at site 300. Revision 2

    Energy Technology Data Exchange (ETDEWEB)

    Kilmer, J.

    1997-08-01

    Various Department of Energy Orders incorporate by reference, health and safety regulations promulgated by the Occupational Safety and Health Administration (OSHA). One of the OSHA regulations, 29 CFR 1910.120, Hazardous Waste Operations and Emergency Response, requires that site safety plans are written for activities such as those covered by work plans for Site 300 environmental investigations. Based upon available data, this Site Safety Plan (Plan) for environmental restoration has been prepared specifically for the Lawrence Livermore National Laboratory Site 300, located approximately 15 miles east of Livermore, California. As additional facts, monitoring data, or analytical data on hazards are provided, this Plan may need to be modified. It is the responsibility of the Environmental Restoration Program and Division (ERD) Site Safety Officer (SSO), with the assistance of Hazards Control, to evaluate data which may impact health and safety during these activities and to modify the Plan as appropriate. This Plan is not `cast-in-concrete.` The SSO shall have the authority, with the concurrence of Hazards Control, to institute any change to maintain health and safety protection for workers at Site 300.

  4. Update of Earthquake Strong-Motion Instrumentation at Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Murray, Robert C. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2013-09-01

    Following the January 1980 earthquake that was felt at Lawrence Livermore National Laboratory (LLNL), a network of strong-motion accelerographs was installed at LLNL. Prior to the 1980 earthquake, there were no accelerographs installed. The ground motion from the 1980 earthquake was estimated from USGS instruments around the Laboratory to be between 0.2 – 0.3 g horizontal peak ground acceleration. These instruments were located at the Veterans Hospital, 5 miles southwest of LLNL, and in San Ramon, about 12 miles west of LLNL. In 2011, the Department of Energy (DOE) requested to know the status of our seismic instruments. We conducted a survey of our instrumentation systems and responded to DOE in a letter. During this survey, it was found that the recorders in Buildings 111 and 332 were not operational. The instruments on Nova had been removed, and only three of the 10 NIF instruments installed in 2005 were operational (two were damaged and five had been removed from operation at the request of the program). After the survey, it was clear that the site seismic instrumentation had degraded substantially and would benefit from an overhaul and more attention to ongoing maintenance. LLNL management decided to update the LLNL seismic instrumentation system. The updated system is documented in this report.

  5. Proposal for FY86 Lawrence Livermore National Laboratory particle beam research

    Science.gov (United States)

    Smith, A. C.

    1990-03-01

    This document represents a combination FY 85 annual report plus the FY 96 proposal for Charged Particle Beam (CPB) research by the Lawrence Livermore National Laboratory's (LLNL's) Beam Research Program. Our report on FY 85 activities focuses on a set of experiments in which the ATA electron beam was propagated through a 1-foot-diameter, 8.5-m-long tank at pressures ranging from the ion-focused regime up to 500 Torr of dry, synthetic air. Based on the optimistic results from this first set of experiments, we propose in FY 86 to extend the tank length to 20 m. We will operate ATA at higher beam current to improve our understanding of intra-accelerator transport physics and to resolve, to our satisfaction, lead pulse stability issues centering around the beam's robustness against the hose instability as a function of beam conditioning. We expect these experiments to yield a wealth of detailed experimental propagation data which will help us prepare for propagating the ATA beam into the open air, an event planned for the end of FY 86. The principal elements are ATA operating time, constructing a flexible lead pulse/conductivity channel tracking facility, and preparing the atmospheric air line for the open air propagation tests. We believe our plans reflect and intelligent and practical symbiotic relationship with SDIO-sponsored Free Electron Laser experiments, also planned on ATA for FY 86, but with very different electron beam current and emittance requirements.

  6. Final Clean Closure Report Site 300 Surface Impoundments Closure Lawrence Livermore National Laboratory Livermore, California

    Energy Technology Data Exchange (ETDEWEB)

    Haskell, K

    2006-02-14

    Lawrence Livermore National Laboratory operated two Class II surface impoundments that stored wastewater that was discharged from a number of buildings located on the Site 300 Facility (Site 300). The wastewater was the by-product of explosives processing. Reduction in the volume of water discharged from these buildings over the past several years significantly reduced the wastewater storage needs. In addition, the impoundments were constructed in 1984, and the high-density polyethylene (HDPE) geomembrane liners were nearing the end of their service life. The purpose of this project was to clean close the surface impoundments and provide new wastewater storage using above ground storage tanks at six locations. The tanks were installed and put into service prior to closure of the impoundments. This Clean Closure Report (Closure Report) complies with State Water Resources Control Board (SWRCB) Section 21400 of the California Code of Regulations Title 27 (27 CCR section 21400). As required by these regulations and guidance, this Closure Report provides the following information: (1) a brief site description; (2) the regulatory requirements relevant to clean closure of the impoundments; (3) the closure procedures; and (4) the findings and documentation of clean closure.

  7. Estimating The Reliability of the Lawrence Livermore National Laboratory (LLNL) Flash X-ray (FXR) Machine

    Energy Technology Data Exchange (ETDEWEB)

    Ong, M M; Kihara, R; Zentler, J M; Kreitzer, B R; DeHope, W J

    2007-06-27

    At Lawrence Livermore National Laboratory (LLNL), our flash X-ray accelerator (FXR) is used on multi-million dollar hydrodynamic experiments. Because of the importance of the radiographs, FXR must be ultra-reliable. Flash linear accelerators that can generate a 3 kA beam at 18 MeV are very complex. They have thousands, if not millions, of critical components that could prevent the machine from performing correctly. For the last five years, we have quantified and are tracking component failures. From this data, we have determined that the reliability of the high-voltage gas-switches that initiate the pulses, which drive the accelerator cells, dominates the statistics. The failure mode is a single-switch pre-fire that reduces the energy of the beam and degrades the X-ray spot-size. The unfortunate result is a lower resolution radiograph. FXR is a production machine that allows only a modest number of pulses for testing. Therefore, reliability switch testing that requires thousands of shots is performed on our test stand. Study of representative switches has produced pre-fire statistical information and probability distribution curves. This information is applied to FXR to develop test procedures and determine individual switch reliability using a minimal number of accelerator pulses.

  8. Building an internet-based workflow system - the case of Lawrence Livermore National Laboratories` Zephyr project

    Energy Technology Data Exchange (ETDEWEB)

    Jordan, C. W., LLNL

    1998-04-01

    Lawrence Livermore National Laboratories` Zephyr System provides a showcase for the ways in which emerging technologies can help streamline procurement processes and improve the coordination between participants in engineering projects by allowing collaboration in ways that have not been possible before. The project also shows the success of a highly pragmatic approach that was initiated by the end user community, and that intentionally covered standard situations, rather than aiming at also automating the exceptions. By helping push purchasing responsibilities down to the end user, thereby greatly reducing the involvement of the purchasing department in operational activities, it was possible to streamline the process significantly resulting in time savings of up to 90%, major cost reductions, and improved quality. Left with less day-to- day purchasing operations, the purchasing department has more time for strategic tasks such as selecting and pre-qualifying new suppliers, negotiating blanket orders, or implementing new procurement systems. The case shows once more that the use of information technologies can result in major benefits when aligned with organizational adjustments.

  9. Novel brominated flame retardants and dechloranes in three fish species from the St. Lawrence River, Canada.

    Science.gov (United States)

    Houde, Magali; Berryman, David; de Lafontaine, Yves; Verreault, Jonathan

    2014-05-01

    Restrictions in the utilization of polybrominated diphenyl ether (PBDE) mixtures have led to the increased usage of alternative flame retardant additives in a wide range of commercial applications. The present study examined the occurrence of established and emerging flame retardants (FRs) in fish from a densely-populated urbanized sector of the St. Lawrence River (Montreal, Quebec, Canada). Thirty-eight PBDE congeners and sixteen emerging FRs were determined in fish belonging to three predatory species (yellow perch, northern pike, and muskellunge). The ∑PBDE in fish were up to 24,115 ng/g lipid weight (l.w.) in the apex predator muskellunge. Twelve emerging FRs including bis(2-ethylhexyl)-tetrabromophthalate (BEHTBP), pentabromoethylbenzene (PBEB), Dechlorane Plus (anti and syn), dechloranes (Dec) 602, Dec 604, Dec 604 Compound B (Dec 604 CB), and Chlordene Plus (CP) were detected (>0.01 ng/gl.w.) in the liver of muskellunge and northern pike but not in yellow perch homogenates. This is the first report of Dec 604 CB in any fish species. The bioavailability of these FRs in human-impacted aquatic ecosystems warrants further environmental assessment and toxicity testing.

  10. Climate systems modeling on massively parallel processing computers at Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Wehner, W.F.; Mirin, A.A.; Bolstad, J.H. [and others

    1996-09-01

    A comprehensive climate system model is under development at Lawrence Livermore National Laboratory. The basis for this model is a consistent coupling of multiple complex subsystem models, each describing a major component of the Earth`s climate. Among these are general circulation models of the atmosphere and ocean, a dynamic and thermodynamic sea ice model, and models of the chemical processes occurring in the air, sea water, and near-surface land. The computational resources necessary to carry out simulations at adequate spatial resolutions for durations of climatic time scales exceed those currently available. Distributed memory massively parallel processing (MPP) computers promise to affordably scale to the computational rates required by directing large numbers of relatively inexpensive processors onto a single problem. We have developed a suite of routines designed to exploit current generation MPP architectures via domain and functional decomposition strategies. These message passing techniques have been implemented in each of the component models and in their coupling interfaces. Production runs of the atmospheric and oceanic components performed on the National Environmental Supercomputing Center (NESC) Cray T3D are described.

  11. Lawrence Livermore National Laboratory East Avenue/Emergency Response Planning Traffic Study

    Energy Technology Data Exchange (ETDEWEB)

    Schmiegel, T

    2002-02-15

    The Lawrence Livermore National Laboratory (LLNL) and the Sandia National Laboratory (SNL) are located at the eastern end of the City of Livermore. Recently the research facilities have been placed on heightened security alerts due to the events of September 11. To respond to the security concerns, LLNL and SNL have proposed to place East Avenue between South Vasco Road and Greenville Road under administrative control. This type of control would require security check points at both ends of this segment of East Avenue, including a truck inspection facility west of the Greenville Road intersection. In this configuration, East Avenue would be closed to general public traffic. The purpose of this traffic study is to determine the potential traffic impact of placing East Avenue under administrative control. The primary focus of the traffic study is to evaluate the proposed modifications to the geometry and operation of East Avenue between South Vasco Road and Greenville Road. The study also includes a review of a traffic study prepared for the closure of East Avenue in 1989 to determine if the assumptions and conclusions of that study remain valid.

  12. Cancer risks from soil emissions of volatile organic compounds at the Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Dibley, V. R., LLNL

    1998-02-01

    The emission isolation flux chamber (EIFC) methodology was applied to Superfund investigations at the Lawrence Livermore National Laboratory Site 300 to determine if on-site workers were exposed to VOCs volatilizing from the subsurface and what, if any, health risks could be attributed to the inhalation of the VOCs volatilizing from the subsurface. During July and August of 1996, twenty, eighteen, and twenty six VOC soil vapor flux samples were collected in the Building 830, 832, and 854 areas, respectively using EIFCS. The VOC concentrations in the vapor samples were used to calculate soil flux rates which were used as input into an air dispersion model to calculate ambient air exposure-point concentrations. The exposure-point concentrations were compared to EPA Region IX Preliminary Remediation Goals (PRGs). Buildings 830 and 832 exposure-point concentrations were less then the PRGs therefore no cancer risks were calculated. The cancer risks for Building 854 ranged from 1.6 x 10{sup -7} to 2.1 x 10{sup -6}. The resultant inhalation cancer risks were all within the acceptable range, implying that on-site workers were not exposed to VOC vapors volatilizing from the subsurface soil that could have significant cancer risks. Therefore remediation in these areas would not be necessary.

  13. Lawrence Livermore National Laboratory underground coal gasification data base. [US DOE-supported field tests; data

    Energy Technology Data Exchange (ETDEWEB)

    Cena, R. J.; Thorsness, C. B.

    1981-08-21

    The Department of Energy has sponsored a number of field projects to determine the feasibility of converting the nation's vast coal reserves into a clean efficient energy source via underground coal gasification (UCG). Due to these tests, a significant data base of process information has developed covering a range of coal seams (flat subbituminous, deep flat bituminous and steeply dipping subbituminous) and processing techniques. A summary of all DOE-sponsored tests to data is shown. The development of UCG on a commercial scale requires involvement from both the public and private sectors. However, without detailed process information, accurate assessments of the commercial viability of UCG cannot be determined. To help overcome this problem the DOE has directed the Lawrence Livermore National Laboratory (LLNL) to develop a UCG data base containing raw and reduced process data from all DOE-sponsored field tests. It is our intent to make the data base available upon request to interested parties, to help them assess the true potential of UCG.

  14. A Comparison between Local and Global Spaceborne Chlorophyll Indices in the St. Lawrence Estuary

    Directory of Open Access Journals (Sweden)

    Martin A. Montes-Hugo

    2012-11-01

    Full Text Available Spaceborne chlorophyll indices based on red fluorescence (wavelength = 680 nm and water leaving radiance (Lw in the visible spectrum (i.e., 400–700 nm were evaluated in the St Lawrence Estuary (SLE during September of 2011. Relationships between chlorophyll concentration (chl and fluorescence were constructed based on fluorescence line height (FLH measurements derived from a compact laser-based spectrofluorometer developed by ENEA (CASPER and using spectral bands corresponding to the satellite sensor MERIS (MEdium Resolution Imaging Spectrometer. Chlorophyll concentration as estimated from CASPER (chlCASPER was relatively high NE of the MTZ (upper Estuary, and nearby areas influenced by fronts or freshwater plumes derived from secondary rivers (lower estuary. These findings agree with historical shipboard measurements. In general, global chl products calculated from Lw had large biases (up to 27-fold overestimation and 50-fold underestimation with respect to chlCASPER values. This was attributed to the smaller interference of detritus (mineral + organic non-living particulates and chromophoric dissolved organic matter on chlCASPER estimates. We encourage the use of spectrofluorometry for developing and validating remote sensing models of chl in SLE waters and other coastal environments characterized by relatively low to moderate (<10 g·m−3 concentrations of detritus.

  15. Holocene development of parabolic dunes in the central St. Lawrence Lowland, Québec

    Science.gov (United States)

    Filion, Louise

    1987-09-01

    Stabilized parabolic dunes in the central St. Lawrence Lowland are oriented NE-SW, in the postulated direction of dune-building winds coming from anticyclonic air circulation induced by the retreating Laurentide Ice Sheet about 10,000 yr ago. The eolian chronology reconstructed from several sections in mixed dune-peatland environments indicates that postglacial plant colonization, characterized by a fortuitous assemblage of arctic-subarctic and boreal elements, preceded dune formation during Champlain Sea regression around 10,000 yr B.P. Confined peatlands and small forests were buried by eolian sands between 10,000 and 7500 yr B.P. under dry and temperate conditions. This eolian episode lasted about 2500 14C yr and ended when cyclonic air circulation similar to the present humid climatic regime was established following the breakup and disappearance of the Laurentide Ice Sheet over Hudson Bay and peripheral areas. Dune stabilization, through paludification of well-drained eolian sands about 7500 yr B.P., suggests a major shift in climate toward wetter conditions that have been characteristic during most of the Holocene in eastern North America. Minor eolian erosion induced by wildfire was recorded during late Holocene time (about 1250 yr B.P.). Anthropogenic perturbation (logging and agriculture practice) was also responsible for recent very local eolian activity.

  16. National strategic challenges and the role of Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Al-Ayat, R.A.; Chrzanowski, P.L.; Werne, R.W.

    1995-01-01

    The end of the Cold War was a water-shed event in history--an event that calls for re-evaluation of the basic assumptions and priorities of US national security that have gone essentially unchallenged for nearly 50 years. Central to this re-evaluation are the changing needs for federal Science and Technology (S and T) investment to underpin national and economic security and the role of the Department of Energy (DOE) national laboratories in fulfilling those needs. The three nuclear weapons laboratories-Los Alamos National Laboratory (LANL), Lawrence Livermore National Laboratory (LLNL), and Sandia National Laboratory (SNL)-are major constituents of DOE`s national laboratory system. They helped win the Cold War, and will undoubtedly continue to support US security S and T requirements. This paper discusses of the role these three laboratories, and LLNL in particular, can play in supporting the nation`s S and T priorities. The paper also highlights some of the changes that are necessary for the laboratories to effectively support the national S and T and economic competitiveness agenda. These issues are important to DOE and laboratory managers responsible for the development of strategic direction and implementation plans.

  17. Mitigation Monitoring Program at Lawrence Livermore National Laboratory FY00 Annual Report

    Energy Technology Data Exchange (ETDEWEB)

    Mcguff, R R

    2003-12-01

    Lawrence Livermore National Laboratory (LLNL) has completed eight years of implementing the mitigation measures from the Final Environmental Impact Statement/Environmental Impact Report (EIS/EIR) for the Continued Operation of LLNL and Sandia National Laboratories (SNL), Livermore. This eighth annual report documents LLNL's implementation of the mitigation measures during the fiscal year ending September 30, 2000 (FY00). It provides background information on the mitigation measures, describes activities undertaken during FY00, and documents changes in the monitoring program. Table 1 on page 12, provides a numerical listing of each mitigation measure, the department responsible for implementing it, and the location within this report where the status is discussed. The discussion of the mitigation measures is organized by the University of California (UC)'s three categories of approaches to implementation: project-specific, service-level and administrative. Table 2 on page 19, Table 6 on page 55, and Table 7 on page 63 provide a detailed discussion of each mitigation measure, including LLNL's implementation strategy and the status as of the end of the fiscal year. Table 3 on page 37, Table 4 on page 46, and Table 5 on page 47 list each construction project undertaken in FY00 and the mitigation measures implemented.

  18. Workplace investigation of increased diagnosis of malignant melanoma among employees of Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Moore, D.H. II; Patterson, H.W.; Hatch, F.; Discher, D.; Schneider, J.S.; Bennett, D.

    1994-08-01

    Based on rates for the surrounding communities, the diagnosis rate of malignant melanoma for employees of Lawrence Livermore National Laboratory (LLNL) during 1972 to 1977 was three to four times higher than expected. In 1984 Austin and Reynolds concluded, as a result of a case-control study, that five occupational factors were {open_quotes}causally associated{close_quotes} with melanoma risk at LLNL. These factors were: (1) exposure to radioactive materials, (2) work at Site 300, (3) exposure to volatile photographic chemicals, (4) presence at the Pacific Test Site, and (5) chemist duties. Subsequent reviews of the Austin and Reynolds report concluded that the methods used were appropriate and correctly carried out. These reports did determine, however, that Austin and Reynolds` conclusion concerning a causal relationship between occupational factors and melanoma among employees was overstated. There is essentially no supporting evidence linking the occupational factors with melanoma from animal studies or human epidemiology. Our report summarizes the results of further investigation of potential occupational factors.

  19. Public census data on CD-ROM at Lawrence Berkeley Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Merrill, D.W.

    1992-10-01

    The Comprehensive Epidemiologic Data Resource (CEDR) and Populations at Risk to Environmental Pollution (PAREP) projects, of the Information and Computing Sciences Division (ICSD) at Lawrence Berkeley Laboratory (LBL), are using public socio-economic and geographic data files which are available to CEDR and PAREP collaborators via LBL`s computing network. At this time 70 CD-ROM diskettes (approximately 36 gigabytes) are on line via the Unix file server cedrcd. lbl. gov. Most of the files are from the US Bureau of the Census, and most pertain to the 1990 Census of Population and Housing. All the CD-ROM diskettes contain documentation in the form of ASCII text files. Printed documentation for most files is available for inspection at University of California Data and Technical Assistance (UC DATA), or the UC Documents Library. Many of the CD-ROM diskettes distributed by the Census Bureau contain software for PC compatible computers, for easily accessing the data. Shared access to the data is maintained through a collaboration among the CEDR and PAREP projects at LBL, and UC DATA, and the UC Documents Library. Via the Sun Network File System (NFS), these data can be exported to Internet computers for direct access by the user`s application program(s).

  20. Public census data on CD-ROM at Lawrence Berkeley Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Merrill, D.W.

    1992-10-01

    The Comprehensive Epidemiologic Data Resource (CEDR) and Populations at Risk to Environmental Pollution (PAREP) projects, of the Information and Computing Sciences Division (ICSD) at Lawrence Berkeley Laboratory (LBL), are using public socio-economic and geographic data files which are available to CEDR and PAREP collaborators via LBL's computing network. At this time 70 CD-ROM diskettes (approximately 36 gigabytes) are on line via the Unix file server cedrcd. lbl. gov. Most of the files are from the US Bureau of the Census, and most pertain to the 1990 Census of Population and Housing. All the CD-ROM diskettes contain documentation in the form of ASCII text files. Printed documentation for most files is available for inspection at University of California Data and Technical Assistance (UC DATA), or the UC Documents Library. Many of the CD-ROM diskettes distributed by the Census Bureau contain software for PC compatible computers, for easily accessing the data. Shared access to the data is maintained through a collaboration among the CEDR and PAREP projects at LBL, and UC DATA, and the UC Documents Library. Via the Sun Network File System (NFS), these data can be exported to Internet computers for direct access by the user's application program(s).

  1. Arroyo Mocho Boulder Removal Project: Lawrence Livermore National Laboratory Hetch Hetchy Pump Station

    Energy Technology Data Exchange (ETDEWEB)

    Burkholder, L; Kato, T; Van Hattem, M

    2007-06-28

    The purpose of this biological assessment is to review the proposed Arroyo Mocho Boulder Removal Project in sufficient detail to determine to what extent the proposed action may affect any of the threatened, endangered, proposed, or sensitive species and designated or proposed critical habitats listed below. In addition, the following information is provided to comply with statutory requirements to use the best scientific and commercial information available when assessing the risks posed to listed and/or proposed species and designated and/or proposed critical habitat by proposed federal actions. This biological assessment is prepared in accordance with legal requirements set forth under regulations implementing Section 7 of the Endangered Species Act (50 CFR 402; 16 U.S.C 1536 (c)). It is our desire for the Arroyo Mocho Boulder Removal Project to receive incidental take coverage for listed species and critical habitat within the greater project area by means of amending the previous formal Section 7 consultation (1-1-04-F-0086) conducted a few hundred meters downstream by Lawrence Livermore National Laboratory (LLNL) in 2002. All conservation measures, terms and conditions, and reporting requirements from the previous Biological Opinion (1-1-04-F-0086) have been adopted for this Biological Assessment and/or amendment.

  2. miRNAting control of DNA methylation

    Indian Academy of Sciences (India)

    Ashwani Jha; Ravi Shankar

    2014-06-01

    DNA methylation is a type of epigenetic modification where a methyl group is added to the cytosine or adenine residue of a given DNA sequence. It has been observed that DNA methylation is achieved by some collaborative agglomeration of certain proteins and non-coding RNAs. The assembly of IDN2 and its homologous proteins with siRNAs recruits the enzyme DRM2, which adds a methyl group at certain cytosine residues within the DNA sequence. In this study, it was found that de novo DNA methylation might be regulated by miRNAs through systematic targeting of the genes involved in DNA methylation. A comprehensive genome-wide and system-level study of miRNA targeting, transcription factors, DNA-methylation-causing genes and their target genes has provided a clear picture of an interconnected relationship of all these factors which regulate DNA methylation in Arabidopsis. The study has identified a DNA methylation system that is controlled by four different genes: IDN2, IDNl1, IDNl2 and DRM2. These four genes along with various critical transcription factors appear to be controlled by five different miRNAs. Altogether, DNA methylation appears to be a finely tuned process of opposite control systems of DNA-methylation-causing genes and certain miRNAs pitted against each other.

  3. Saginaw Bay, MI LiDAR

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — TASK NAME:(NRCS) Saginaw Bay, MI LiDAR LiDAR Data Acquisition and Processing Production Task USGS Contract No. G10PC00057 Task Order No. G11PD01254 Woolpert Order...

  4. 78 FR 36631 - Michigan Disaster #MI-00039

    Science.gov (United States)

    2013-06-18

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Michigan Disaster MI-00039 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY... INFORMATION CONTACT: A. Escobar, Office of Disaster Assistance, U.S. Small Business Administration, 409...

  5. Mini Networked Screens (MiNeS)

    NARCIS (Netherlands)

    Prins, C.A.; Maris, M.; Breen, P.C.; Versteeg, N.; Terwisga, P.F. van; Ort, C.M.; Blok, J.J.

    2005-01-01

    The forward areas for an LPD in littoral waters can be full of surprises. A novel concept is presented for a networked screen consisting of elements of increasing capability to provide a progressive response to the threat. This MiNeS concept substantially improves the capability of the LPD as an aut

  6. Altered Expressions of miR-1238-3p, miR-494, miR-6069, and miR-139-3p in the Formation of Chronic Brucellosis

    Science.gov (United States)

    Budak, Ferah; Bal, Salih Haldun; Tezcan, Gulcin; Akalın, Halis; Goral, Guher

    2016-01-01

    Brucellosis is a zoonotic disease that is still endemic in developing countries. Despite early diagnosis and treatment of patients, chronic infections are seen in 10–30% of patients. In this study, we aimed to investigate the immunological factors that play roles in the transition of brucellosis from acute infection into chronic infection. Here, more than 2000 miRNAs were screened in peripheral blood mononuclear cells (PBMCs) of patients with acute or chronic brucellosis and healthy controls by using miRNA array, and the results of the miRNA array were validated through qRT-PCR. Findings were evaluated using GeneSpring GX (Agilent) 13.0 software and KEGG pathway analysis. Four miRNAs were expressed in the chronic group but were not expressed in acute and control groups. Among these miRNAs, the expression level of miR-1238-3p was increased while miR-494, miR-6069, and miR-139-3p were decreased (p 2). These miRNAs have the potential to be markers for chronic cases. The differentially expressed miRNAs and their predicted target genes involved in endocytosis, regulation of actin cytoskeleton, MAPK signaling pathway, and cytokine-cytokine receptor interaction and its chemokine signaling pathway indicate their potential roles in chronic brucellosis and its progression. It is the first study of miRNA expression analysis of human PBMC to clarify the mechanism of inveteracy in brucellosis. PMID:27722176

  7. Altered Expressions of miR-1238-3p, miR-494, miR-6069, and miR-139-3p in the Formation of Chronic Brucellosis.

    Science.gov (United States)

    Budak, Ferah; Bal, Salih Haldun; Tezcan, Gulcin; Akalın, Halis; Goral, Guher; Oral, Haluk Barbaros

    2016-01-01

    Brucellosis is a zoonotic disease that is still endemic in developing countries. Despite early diagnosis and treatment of patients, chronic infections are seen in 10-30% of patients. In this study, we aimed to investigate the immunological factors that play roles in the transition of brucellosis from acute infection into chronic infection. Here, more than 2000 miRNAs were screened in peripheral blood mononuclear cells (PBMCs) of patients with acute or chronic brucellosis and healthy controls by using miRNA array, and the results of the miRNA array were validated through qRT-PCR. Findings were evaluated using GeneSpring GX (Agilent) 13.0 software and KEGG pathway analysis. Four miRNAs were expressed in the chronic group but were not expressed in acute and control groups. Among these miRNAs, the expression level of miR-1238-3p was increased while miR-494, miR-6069, and miR-139-3p were decreased (p 2). These miRNAs have the potential to be markers for chronic cases. The differentially expressed miRNAs and their predicted target genes involved in endocytosis, regulation of actin cytoskeleton, MAPK signaling pathway, and cytokine-cytokine receptor interaction and its chemokine signaling pathway indicate their potential roles in chronic brucellosis and its progression. It is the first study of miRNA expression analysis of human PBMC to clarify the mechanism of inveteracy in brucellosis.

  8. Inga Lindströmi edu saladus / Jaanus Noormets

    Index Scriptorium Estoniae

    Noormets, Jaanus

    2009-01-01

    2004. aastal alustas Saksamaa telekanal ZDF romantiliste telefilmide "Inga Lindströmi armastuslood" filmide näitamist. Inga Lindströmi pseudonüümi kasutab stsenarist Christiane Sadlo. Seriaal on teinud suure töö Rootsi reklaamimisel reisisihina

  9. Cortical Morphogenesis during Embryonic Development Is Regulated by miR-34c and miR-204

    Science.gov (United States)

    Venø, Morten T.; Venø, Susanne T.; Rehberg, Kati; van Asperen, Jessy V.; Clausen, Bettina H.; Holm, Ida E.; Pasterkamp, R. Jeroen; Finsen, Bente; Kjems, Jørgen

    2017-01-01

    The porcine brain closely resembles the human brain in aspects such as development and morphology. Temporal miRNA profiling in the developing embryonic porcine cortex revealed a distinct set of miRNAs, including miR-34c and miR-204, which exhibited a highly specific expression profile across the time of cortical folding. These miRNAs were found to target Doublecortin (DCX), known to be involved in neuron migration during cortical folding of gyrencephalic brains. In vivo modulation of miRNA expression in mouse embryos confirmed that miR-34c and miR-204 can control neuronal migration and cortical morphogenesis, presumably by posttranscriptional regulation of DCX. PMID:28232790

  10. 小鼠着床前胚胎miR-125a,miR-295和miR-181b的表达%Expression of miR-125a, miR-295 and miR-181b in mouse preimplantation embryos

    Institute of Scientific and Technical Information of China (English)

    张平; 杨艳红; 王晓红; 王珺; 姜峰; 罗亚宁; 姚元庆

    2008-01-01

    目的:研究小鼠着床前胚胎发育过程中miRNA表达水平的变化,探讨miRNA对着床前胚胎发育过程的作用. 方法:建立小鼠超排、交配系统,收集着床前胚胎,提取small RNA;采用miRNA特异RT引物进行反转录,采用SYBR Green实时定量PCR技术,研究miR-125a, miR-295和miR-181b在小鼠着床前胚胎发育过程中表达水平的变化. 结果:着床前胚胎发育的各阶段均表达miR-125a, miR-295和miR-181b;随着胚胎发育进程,miR-295的表达呈逐渐上升的趋势;miR-125a在卵母细胞到2细胞发育阶段呈下降趋势,以后随发育进程呈逐渐上升趋势. 结论:小鼠着床前胚胎中表达miRNA,着床前胚胎的发育和分化过程可能受到miRNA的调控.

  11. miR319, miR390, and miR393 Are Involved in Aluminum Response in Flax (Linum usitatissimum L.)

    Science.gov (United States)

    Zyablitsin, Alexander V.; Rozhmina, Tatiana A.; Speranskaya, Anna S.; Sadritdinova, Asiya F.

    2017-01-01

    Acid soils limit agricultural production worldwide. Major reason of crop losses in acid soils is the toxicity of aluminum (Al). In the present work, we investigated expression alterations of microRNAs in flax (Linum usitatissimum L.) plants under Al stress. Flax seedlings of resistant (TMP1919 and G1071/4_k) and sensitive (Lira and G1071/4_o) to Al cultivars and lines were exposed to AlCl3 solution for 4 and 24 hours. Twelve small RNA libraries were constructed and sequenced using Illumina platform. In total, 97 microRNAs from 18 conserved families were identified. miR319, miR390, and miR393 revealed expression alterations associated with Al treatment of flax plants. Moreover, for miR390 and miR393, the alterations were distinct in sensitive and resistant to Al genotypes. Expression level changes of miR319 and miR390 were confirmed using qPCR analysis. In flax, potential targets of miR319 are TCPs, miR390–TAS3 and GRF5, and miR393–AFB2-coding transcripts. TCPs, TAS3, GRF5, and AFB2 participate in regulation of plant growth and development. The involvement of miR319, miR390, and miR393 in response to Al stress in flax was shown here for the first time. We speculate that these microRNAs play an important role in Al response via regulation of growth processes in flax plants.

  12. Relationship between arterial atheromatous plaque morphology and platelet-associated miR-126 and miR-223 expressions

    Institute of Scientific and Technical Information of China (English)

    Heng-Song Tian; Qing-Guo Zhou; Fang Shao

    2015-01-01

    Objective: To study the expression of miR-126 and miR-223 in platelet of rabbit arterial plaque models, and explore its correlation with plaque morphology. Methods: Rabbit arterial plaque models were established, peripheral blood of models and control animals was collected. Plaque morphologies were divided into type Ⅰ, type Ⅱ and type Ⅲ based on angiography plaque morphology and Ambrose method. Platelet isolation kit was applied to isolate and purify peripheral blood platelets, CD45 immunomagnetic beads were used to remove the residual white blood cells. The miRNAs of platelets was extracted by miRNA Isolation Kit, and expressions of miR-126 and miR-223 of the platelets samples were detected by Real-time PCR. The correlation between plaque morphology and platelet-associated miR-126 and miR-223 expressions were analyzed. Expressions of target gene VCAM-1 and P2Y12 receptors of miR-126 and miR-223 in the atherosclerosis plaque of rabbit model were detected by Western blot. Results: Relative expression levels of miR-126 and miR-223 in the model group were 0.27±0.10 and 0.71±0.14, respectively. Plaque morphology was divided into types Ⅰ, Ⅱ and Ⅲ;and miR-126 and miR-223 expression levels were detected in each type. Expression levels of miR-126 in each type were 0.42±0.07, 0.17±0.11 and 0.22±0.15, respectively; and expression levels of miR-223 in each type are 0.68±0.02, 0.57±0.06 and 0.88±0.10, respectively. Relative to the control group, miR-126 and miR-223 known target genes in VCAM-1 and P2Y12 receptors increased platelets in rabbit atherosclerotic plaque models (P<0.05). Conclusions:Relative to normal control animals, miR-126 and miR-223 platelets were reduced in the rabbit atherosclerotic plaque model group (P<0.05). In the type Ⅱ plaque morphology group, miR-126 was greatly reduced; and there is no significant correlation between miR-223 and plaque morphology.

  13. Analysis of the hydraulic data from the MI fracture zone at the Grimsel Rock Laboratory, Switzerland

    Energy Technology Data Exchange (ETDEWEB)

    Davey, A.; Karasaki, K.; Long, J.C.S.; Landsfeld, M.; Mensch, A.; Martel, S.J.

    1989-10-01

    One of the major problems in analyzing flow and transport in fractured rock is that the flow may be largely confined to a poorly connected network of fractures. In order to overcome some of this problem, Lawrence Berkeley Laboratory (LBL) has been developing a new type of fracture hydrology model called an equivalent discontinuum model. In this model the authors represent the discontinuous nature of the problem through flow on a partially filled lattice. A key component in constructing an equivalent discontinuum model from this lattice is removing some of the conductive elements such that the system is partially connected in the same manner as the fracture network. This is done through a statistical inverse technique called simulated annealing. The fracture network model is annealed by continually modifying a base model, or template such that the modified systems behave more and more like the observed system. In order to see how the simulated annealing algorithm works, the authors have developed a series of synthetic real cases. In these cases, the real system is completely known so that the results of annealing to steady state data can be evaluated absolutely. The effect of the starting configuration has been studied by varying the percent of conducting elements in the initial configuration. Results have shown that the final configurations converge to about the same percentage of conducting elements. An example using Nagra field data from the Migration Experiment (MI) at Grimsel Rock Laboratory in Switzerland is also analyzed. 24 refs., 33 figs., 3 tabs.

  14. Polarimetry of comets 67P/Churyumov-Gerasimenko, 74P/Smirnova-Chernykh, and 152P/Helin-Lawrence

    CERN Document Server

    Stinson, A; Tozzi, G P; Boehnhardt, H; Protopapa, S; Kolokolova, L; Muinonen, K; Jones, G H

    2016-01-01

    Aims. Polarimetric characteristics of comets at large heliocentric distances is a relatively unexplored area; we extend the idea by adding and analysing the data for three Jupiter family comets (JFCs). Methods. With the FORS2 instrument of the ESO VLT, we performed quasi-simultaneous photometry and polarimetry of three active JFCs 67P/Churyumov-Gerasimenko, 74P/Smirnova-Chernykh, and 152P/Helin-Lawrence. Results. We obtained in total 23 polarimetric measurements at different epochs, covering a phase-angle range ~1 -16 degrees and heliocentric distances from 3 to 4.6 au. From our observations we obtained both colour and polarimetric maps to look for structures in the comae and tails of the comets. Conclusions. 74P/Smirnova-Chernykh and 152P/Helin-Lawrence exhibit enough activity at large heliocentric distances to be detectable in polarimetric measurements. Polarimetric and colour maps indicate no evidence of dust particle

  15. Toxoplasmosis in beluga whales (Delphinapterus leucas) from the St Lawrence estuary: two case reports and a serological survey.

    Science.gov (United States)

    Mikaelian, I; Boisclair, J; Dubey, J P; Kennedy, S; Martineau, D

    2000-01-01

    Toxoplasmosis was diagnosed in two free-ranging beluga whales from the St Lawrence estuary, Quebec, Canada, in 1988 and 1998. Histologically, tachyzoites and bradyzoites were present in the brain, spleen, lymph nodes, adrenals and lungs of both animals, and in the thymus of one. These organisms were readily labelled by an indirect immunohistochemical method for Toxoplasma gondii antigens. In the lymph nodes, spleen and lungs the organisms were associated with histiocytic infiltration. In the brain of one animal they were associated with mild multifocal gliosis and haemorrhages. There was no evidence of concomitant morbillivirus infection. Serum samples were collected from 22 beluga whales stranded between 1995 and 1998 on the shores of the St Lawrence Estuary and examined for antibodies to T. gondii by the modified agglutination test. Antibody titres of s25 were found in six (27%) of the animals. This is the first confirmed report of toxoplasmosis in beluga whales.

  16. Stable isotope evidence for glacial lake drainage through the St. Lawrence Estuary, eastern Canada, ~13.1-12.9 ka

    Science.gov (United States)

    Cronin, T. M.; Rayburn, J.A.; Guilbault, J.-P.; Thunell, R.; Franzi, D.A.

    2012-01-01

    Postglacial varved and rhythmically-laminated clays deposited during the transition from glacial Lake Vermont (LV) to the Champlain Sea (CS) record hydrological changes in the Champlain-St. Lawrence Valley (CSLV) at the onset of the Younger Dryas ∼13.1–12.9 ka linked to glacial lake drainage events. Oxygen isotope (δ18O) records of three species of benthic foraminifera (Cassidulina reniforme, Haynesina orbiculare, Islandiella helenae) from six sediment cores and the freshwater ostracode Candona from one core were studied. Results show six large isotope excursions (∼0.5 to >2‰) in C. reniforme δ18O values, five excursions in H. orbiculare (glacial Lake Agassiz, perhaps in a series of floods, ultimately draining out the St. Lawrence Estuary.

  17. Identification of novel miRNAs and miRNA dependent developmental shifts of gene expression in Arabidopsis thaliana.

    Directory of Open Access Journals (Sweden)

    Shuhua Zhan

    Full Text Available microRNAs (miRNAs are small, endogenous RNAs of 20 approximately 25 nucleotides, processed from stem-loop regions of longer RNA precursors. Plant miRNAs act as negative regulators of target mRNAs predominately by slicing target transcripts, and a number of miRNAs play important roles in development. We analyzed a number of published datasets from Arabidopsis thaliana to characterize novel miRNAs, novel miRNA targets, and miRNA-regulated developmental changes in gene expression. These data include microarray profiling data and small RNA (sRNA deep sequencing data derived from miRNA biogenesis/transport mutants, microarray profiling data of mRNAs in a developmental series, and computational predictions of conserved genomic stem-loop structures. Our conservative analyses identified five novel mature miRNAs and seven miRNA targets, including one novel target gene. Two complementary miRNAs that target distinct mRNAs were encoded by one gene. We found that genes targeted by known miRNAs, and genes up-regulated or down-regulated in miRNA mutant inflorescences, are highly expressed in the wild type inflorescence. In addition, transcripts upregulated within the mutant inflorescences were abundant in wild type leaves and shoot meristems and low in pollen and seed. Downregulated transcripts were abundant in wild type pollen and seed and low in shoot meristems, roots and leaves. Thus, disrupting miRNA function causes the inflorescence transcriptome to resemble the leaf and meristem and to differ from pollen and seed. Applications of our computational approach to other species and the use of more liberal criteria than reported here will further expand the number of identified miRNAs and miRNA targets. Our findings suggest that miRNAs have a global role in promoting vegetative to reproductive transitions in A. thaliana.

  18. Plasma levels of miR-10b, miR-122, miR-16 and miR-183 in patients with non-small-cell lung cancer and their relationships with the overall survival%非小细胞肺癌患者血浆miR-10b、miR-122、miR-16和miR-183水平及其与总生存期的关系

    Institute of Scientific and Technical Information of China (English)

    王富民; 张寰; 曲金力; 钱碧云

    2016-01-01

    Objective To observe the levels of miR-10b, miR-122, miR-16 and miR-183 in patients with non-small-cell lung cancer (NSCLC) and to explore their relationships with the overall survival .Methods According to recurrence, 40 NSCLC patients were divided into two groups:non-recurrence group (n=8) and recurrence group (n=32).The ex-pression levels of 4 miRNAs were detected by real time quantitative PCR .NSCLC patients were divided into the high miR-NA expression group and low expression group according to the mean value of miRNAs expression .Kaplan-Meier survival a-nalysis and Cox regression were performed to determine the associations between different levels of miRNAs expression and overall survival ( OS) .Results The expression of miR-183 was higher in the non-recurrence group as compared with that of the recurrence group (P0.05).Cox proportional hazard regression showed that miR-183 was the independent risk factor for the overall survival of NSCLC patients (HR=2.854, 95%CI=1.154-7.055, P0.05. miR-183低水平者OS高于高水平者(P0.05. Cox比例风险回归模型分析显示,miR-183是NSCLC患者OS的独立影响因素( HR=2.854,95%CI为1.154~7.055,P<0.05). 结论 miR-183可能是NSCLC患者OS的独立影响因素,而miR-10b、miR-122和miR-16与患者OS无关.

  19. miR-106b、miR-20a和miR-221在早期胃癌中表达的研究%A study on miR-106b, miR-20a and miR-221 expressions in early gastric cancer

    Institute of Scientific and Technical Information of China (English)

    蔡辉; 袁媛; 韦雪; 魏丽; 王晓鹏; 郝云飞; 张迎梅; 郭天康

    2014-01-01

    Objective To study the exprssion of miR-106b,miR-20a and miR-221 in early development of gastric cancer.Methods The plasma expression of 15 selected microRNAs (miR-1,miR-106a,miR-106b,miR-17-5p,miR-20a,miR-21,miR-221,miR-27a,miR-34,miR-376c,miR-378,miR-423-5p,miR-451,miR-486,miR-744) in 30 gastric cancer (GC) patients and 30 age-and gendermatched healthy controls were measured and then the differences of those micro-RNAs expressions in other 60 GC patients and 60 matched controls were evaluated by using quantitative reverse transcription polymerase chain reaction.The areas under the receiver operating characteristic (ROC) curves were used to test the sensitivity and specificity of GC diagnosis using these identified plasma microRNAs.Results Three plasma microRNAs miR-106b,miR-20a,and miR-221,were significantly elevated in GC patients than in healthy controls (P < 0.05).Furthermore,the areas under the ROC curves using miR-106b,miR-20a,and miR-221 for GC diagnosis were 0.773 (95% CI 0.776-0.841),0.859 (95% CI 0.805-0.914),and 0.796 (95% CI 0.726-0.866),respectively.These three microRNAs were on a statistically significant elevation in GC patients compared with healthy controls at each of the four stages.However,there were no significant differences in the plasma levels of the three microRNAs among the four TNM stages (P > 0.05).Conclusions Plasma miR-106b,miR-20a,and miR-221 may be used as biomarkers for the detection of early GC.%目的 探讨血浆微小RNA在早期胃癌中的表达情况,研究其在胃癌发生发展中的意义.方法 本研究首先对30例胃癌患者和30例健康对照人群血浆中的15种微小RNA(miR-1、miR-106a、miR-106b、miR-17-5p、miR-20a、miR-21、miR-221、miR-27a、miR-34、miR-376c、miR-378、miR-423-5p、miR-451、miR-486、miR-744)进行检测,然后通过定量逆转录聚合酶链反应来确定另外60例胃癌患者和60例健康对照人群的血浆微小RNA是否存在差异.通过受试者工作特征

  20. Institute of Geophysics and Planetary Physics (IGPP), Lawrence Livermore National Laboratory (LLNL): Quinquennial report, November 14-15, 1996

    Energy Technology Data Exchange (ETDEWEB)

    Tweed, J.

    1996-10-01

    This Quinquennial Review Report of the Lawrence Livermore National Laboratory (LLNL) branch of the Institute for Geophysics and Planetary Physics (IGPP) provides an overview of IGPP-LLNL, its mission, and research highlights of current scientific activities. This report also presents an overview of the University Collaborative Research Program (UCRP), a summary of the UCRP Fiscal Year 1997 proposal process and the project selection list, a funding summary for 1993-1996, seminars presented, and scientific publications. 2 figs., 3 tabs.

  1. Lawrence Livermore National Laboratory Experimental Test Site, Site 300, Biological Review, January 1, 2009 through December 31, 2012

    Energy Technology Data Exchange (ETDEWEB)

    Paterson, Lisa E. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Woollett, Jim S. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2014-01-01

    The Lawrence Livermore National Laboratory’s (LLNL’s) Environmental Restoration Department (ERD) is required to conduct an ecological review at least every five years to ensure that biological and contaminant conditions in areas undergoing remediation have not changed such that existing conditions pose an ecological hazard (Dibley et al. 2009a). This biological review is being prepared by the Natural Resources Team within LLNL’s Environmental Functional Area (EFA) to support the 2013 five-year ecological review.

  2. A case-control study of malignant melanoma among Lawrence Livermore National Laboratory employees: A critical evaluation

    Energy Technology Data Exchange (ETDEWEB)

    Kupper, L.L.; Setzer, R.W.; Schwartzbaum, J.; Janis, J.

    1987-07-01

    This document reports on a reevaluation of data obtained in a previous report on occupational factors associated with the development of malignant melanomas at Lawrence Livermore National Laboratory. The current report reduces the number of these factors from five to three based on a rigorous statistical analysis of the original data. Recommendations include restructuring the original questionnaire and trying to contact more individuals that worked with volatile photographic chemicals. 17 refs., 7 figs., 22 tabs. (TEM)

  3. Benthic nutrient fluxes along the Laurentian Channel: Impacts on the N budget of the St. Lawrence marine system

    Science.gov (United States)

    Thibodeau, Benoît; Lehmann, Moritz F.; Kowarzyk, Jacqueline; Mucci, Alfonso; Gélinas, Yves; Gilbert, Denis; Maranger, Roxane; Alkhatib, Mohammad

    2010-12-01

    Water column concentrations and benthic fluxes of dissolved inorganic nitrogen (DIN) and oxygen (DO) were measured in the Gulf of St. Lawrence and the Upper and Lower St. Lawrence Estuary (USLE and LSLE, respectively) to assess the nitrogen (N) budget in the St. Lawrence (SL) system, as well as to elucidate the impact of bottom water hypoxia on fixed-N removal in the LSLE. A severe nitrate deficit, with respect to ambient phosphate concentrations (N*˜-10 μmol L -1), was observed within and in the vicinity of the hypoxic bottom water of the LSLE. Given that DO concentrations in the water column have remained above 50 μmol L -1, nitrate reduction in suboxic sediments, rather than in the water column, is most likely responsible for the removal of fixed N from the SL system. Net nitrate fluxes into the sediments, derived from pore water nitrate concentration gradients, ranged from 190 μmol m -2 d -1 in the hypoxic western LSLE to 100 μmol m -2 d -1 in the Gulf. The average total benthic nitrate reduction rate for the Laurentian Channel (LC) is on the order of 690 μmol m -2 d -1, with coupled nitrification-nitrate reduction accounting for more than 70%. Using average nitrate reduction rates derived from the observed water column nitrate deficit, the annual fixed-N elimination within the three main channels of the Gulf of St. Lawrence and LSLE was estimated at 411 × 10 6 t N, yielding an almost balanced N budget for the SL marine system.

  4. Pathology and toxicology of beluga whales from the St. Lawrence Estuary, Quebec, Canada. Past, present and future.

    Science.gov (United States)

    Martineau, D; De Guise, S; Fournier, M; Shugart, L; Girard, C; Lagacé, A; Béland, P

    1994-09-16

    An indigenous population of 450-500 beluga whales (Delphinapterus leucas) inhabiting the St. Lawrence Estuary has been exposed chronically for more than 50 years to a complex mixture of industrial pollutants including organochlorinated compounds (OC), polycyclic aromatic hydrocarbons (PAH) and heavy metals. From 1983 to 1990, we have necropsied 45 well preserved carcasses out of a total of 120 beluga whales reported dead over this period. Of these 45 animals, nine were affected by 10 malignant neoplasms. Fifteen animals (33%) were affected by pneumonia. Milk production was compromised in eight of 17 mature females (41%), by inflammatory changes (seven animals) and cancer (one animal) which affected the mammary glands. Opportunistic bacteria were found in pure culture, and/or in significant amounts in at least two organs in 20 belugas (44%). The concentrations of both total PCBs and highly chlorinated PCB congeners were much higher in St. Lawrence animals than in Arctic beluga whales. OC-induced immunosuppression has been repeatedly demonstrated in a wide variety of animal species. Therefore, it is probable that the immune functions of St. Lawrence beluga whales are impaired. Benzo[a]pyrene adducts were detected in 10 of the 11 St. Lawrence beluga whales of which tissues (six livers, 10/11 brains) were analyzed by a method based on HPLC. No such adducts were found in four Arctic animals. Since benzo[alpha]pyrene is one of the most potent chemical carcinogens known to man, these compounds might be responsible for some of the cancers observed in that population. Overall, our findings contrast vividly with those of others who found that cancers are exceedingly rare in free-ranging odontocete populations and that the major causes for mortalities in these populations are bacteria, parasites, and trauma.

  5. Cancer in wildlife, a case study: beluga from the St. Lawrence estuary, Québec, Canada.

    OpenAIRE

    Martineau, Daniel; Lemberger, Karin; Dallaire, André; Labelle, Philippe; Lipscomb, Thomas P; Michel, Pascal; Mikaelian, Igor

    2002-01-01

    A population of approximately 650 beluga (Delphinapterus leucas) inhabits a short segment of the St. Lawrence estuary (SLE). Over 17 years (1983-1999), we have examined 129 (or 49%) of 263 SLE beluga carcasses reported stranded. The major primary causes of death were respiratory and gastrointestinal infections with metazoan parasites (22%), cancer (18%), and bacterial, viral, and protozoan infections (17%). We observed cancer in 27% of examined adult animals found dead, a percentage similar t...

  6. miRSeq: A User-Friendly Standalone Toolkit for Sequencing Quality Evaluation and miRNA Profiling

    Directory of Open Access Journals (Sweden)

    Cheng-Tsung Pan

    2014-01-01

    Full Text Available MicroRNAs (miRNAs present diverse regulatory functions in a wide range of biological activities. Studies on miRNA functions generally depend on determining miRNA expression profiles between libraries by using a next-generation sequencing (NGS platform. Currently, several online web services are developed to provide small RNA NGS data analysis. However, the submission of large amounts of NGS data, conversion of data format, and limited availability of species bring problems. In this study, we developed miRSeq to provide alternatives. To test the performance, we had small RNA NGS data from four species, including human, rat, fly, and nematode, analyzed with miRSeq. The alignments results indicate that miRSeq can precisely evaluate the sequencing quality of samples regarding percentage of self-ligation read, read length distribution, and read category. miRSeq is a user-friendly standalone toolkit featuring a graphical user interface (GUI. After a simple installation, users can easily operate miRSeq on a PC or laptop by using a mouse. Within minutes, miRSeq yields useful miRNA data, including miRNA expression profiles, 3′ end modification patterns, and isomiR forms. Moreover, miRSeq supports the analysis of up to 105 animal species, providing higher flexibility.

  7. miR-375 ameliorates sepsis by downregulating miR-21 level via inhibiting JAK2-STAT3 signaling.

    Science.gov (United States)

    Sheng, Bo; Zhao, Lei; Zang, Xuefeng; Zhen, Jie; Chen, Wei

    2017-02-01

    Accumulating evidences have confirmed that miRNAs have important roles in sepsis. Myeloid-derived suppressor cells (MDSCs) enhance late sepsis development through immunosuppression in mice. Here, the functions and mechanisms of miR-375 in sepsis were revealed. We found that miR-375 level was downregulated but miR-21 level was upregulated in sepsis patients and that their levels were correlated negatively. Importantly, ectopic expression of miR-375 could decrease the number of sepsis Gr1+CD11b+ MDSCs in mice. Mechanistically, miR-375 could target Janus kinase 2 (JAK2) and further impaired signal transducer and activator of transcription 3 (STAT3) in sepsis Gr1+CD11b+ MDSC. Gain and loss of function of experiments showed that upregulation or downregulation of miR-375 level could decrease or increase miR-21 level. Moreover, pretreatment of JAK2 overexpressing vector could abolish the effects of miR-375 on miR-21 level and the amount of sepsis Gr1+CD11b+ MDSCs. Therefore, our results demonstrate that miR-375 could block JAK2-STAT3 pathway and thus modulate miR-21 level, which is involved in regulation of late sepsis. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  8. Altering β-cell number through stable alteration of miR-21 and miR-34a expression

    DEFF Research Database (Denmark)

    Backe, Marie Balslev; Novotny, Guy Wayne; Christensen, Dan Ploug;

    2014-01-01

    RNAs, miR-21 and miR-34a, may be involved in mediating cytokine-induced β-cell dysfunction. Therefore, manipulation of miR-21 and miR-34a levels may potentially be beneficial to β cells. To study the effect of long-term alterations of miR-21 or miR-34a levels upon net β-cell number, we stably overexpressed...... miR-21 and knocked down miR-34a, and investigated essential cellular processes. Materials and Methods: miRNA expression was manipulated using Lentiviral transduction of the β-cell line INS-1. Stable cell lines were generated, and cell death, NO synthesis, proliferation, and total cell number were...... monitored in the absence or presence of cytokines. Results: Overexpression of miR-21 decreased net β-cell number in the absence of cytokines, and increased apoptosis and NO synthesis in the absence and presence of cytokines. Proliferation was increased upon miR-21 overexpression. Knockdown of miR-34a...

  9. Polarimetry of comets 67P/Churyumov-Gerasimenko, 74P/Smirnova-Chernykh, and 152P/Helin-Lawrence

    Science.gov (United States)

    Stinson, A.; Bagnulo, S.; Tozzi, G. P.; Boehnhardt, H.; Protopapa, S.; Kolokolova, L.; Muinonen, K.; Jones, G. H.

    2016-10-01

    Aims: Polarimetric characteristics of comets at large heliocentric distances is a relatively unexplored area; we extend the idea by adding and analysing the data for three Jupiter family comets (JFCs). Methods: With the FORS2 instrument of the ESO VLT, we performed quasi-simultaneous photometry and polarimetry of three active JFCs 67P/Churyumov-Gerasimenko, 74P/Smirnova-Chernykh, and 152P/Helin-Lawrence. Results: We obtained in total 23 polarimetric measurements at different epochs, covering a phase-angle range ~1°-16° and heliocentric distances from 3 to 4.6 au. From our observations we obtained both colour and polarimetric maps to look for structures in the comae and tails of the comets. Conclusions: 74P/Smirnova-Chernykh and 152P/Helin-Lawrence exhibit enough activity at large heliocentric distances to be detectable in polarimetric measurements. Polarimetric and colour maps indicate no evidence of dust particle evolution in the coma. From near-infrared spectra we find no evidence of water ice in the coma of comet 152P/Helin-Lawrence. Based on observations collected at the European Southern Observatory, Chile, under Programme IDs:089.C-0619 (PI = Tozzi), and 384.C-0115 (PI = Boehnhardt).

  10. Towards a National Hydrological Forecasting system for Canada : Lessons Learned from the Great Lakes and St. Lawrence Prediction System

    Science.gov (United States)

    Fortin, V.; Durnford, D.; Gaborit, E.; Davison, B.; Dimitrijevic, M.; Matte, P.

    2016-12-01

    Environment and Climate Change Canada has recently deployed a water cycle prediction system for the Great Lakes and St. Lawrence River. The model domain includes both the Canadian and US portions of the watershed. It provides 84-h forecasts of weather elements, lake level, lake ice cover and surface currents based on two-way coupling of the GEM numerical weather prediction (NWP) model with the NEMO ocean model. Streamflow of all the major tributaries of the Great Lakes and St. Lawrence River are estimated by the WATROUTE routing model, which routes the surface runoff forecasted by GEM's land-surface scheme and assimilates streamflow observations where available. Streamflow forecasts are updated twice daily and are disseminated through an OGC compliant web map service (WMS) and a web feature service (WFS). In this presentation, in addition to describing the system and documenting its forecast skill, we show how it is being used by clients for various environmental prediction applications. We then discuss the importance of two-way coupling, land-surface and hillslope modelling and the impact of horizontal resolution on hydrological prediction skill. In the second portion of the talk, we discuss plans for implementing a similar system at the national scale, using what we have learned in the Great Lakes and St. Lawrence watershed. Early results obtained for the headwaters of the Saskatchewan River as well as for the whole Nelson-Churchill watershed are presented.

  11. miRNA Isolation from FFPET Specimen: A Technical Comparison of miRNA and Total RNA Isolation Methods.

    Science.gov (United States)

    Nagy, Zsófia Brigitta; Wichmann, Barnabás; Kalmár, Alexandra; Barták, Barbara Kinga; Tulassay, Zsolt; Molnár, Béla

    2016-07-01

    MiRNA remain stable for detection and PCR-based amplification in FFPE tissue samples. Several miRNA extraction kits are available, however miRNA fraction, as part of total RNA can be isolated using total RNA purification methods, as well. Our primary aim was to compare four different miRNA and total RNA isolation methods from FFPE tissues. Further purposes were to evaluate quantitatively and qualitatively the yield of the isolated miRNA. MiRNAs were isolated from normal colorectal cancer FFPE specimens from the same patients. Two miRNA isolation kits (High Pure miRNA Isolation Kit, miRCURY™ RNA Isolation Kit) and two total RNA isolation kits were compared (High Pure RNA Paraffin Kit, MagNA Pure 96 Cellular RNA LV Kit). Quantity and quality were determined, expression analysis was performed by real-time PCR using qPCR Human Panel I + II (Exiqon) method detecting 742 human miRNAs in parallel. The yield of total RNA was found to be higher than miRNA purification protocols (in CRC: Ex: 0203 ± 0021 μg; HPm: 1,45 ± 0,8 μg; HPp: 21,36 ± 4,98 μg; MP: 8,6 ± 5,1 μg). MiRNAs were detected in lower relative quantity of total RNA compared to the miRNA kits. Higher number of miRNAs could be detected by the miRNA isolation kits in comparison to the total RNA isolation methods. (Ex: 497 ± 16; HPm: 542 ± 11; HPp: 332 ± 36; MP: 295 ± 74). Colon specific miRNAs (miR-21-5p;-34-5p) give satisfying results by miRNA isolation kits. Although miRNA can be detected also after total RNA isolation methods, for reliable and reproducible miRNA expression profiling the use of miRNA isolation kits are more suitable.

  12. Identification of miRNAs contributing to neuroblastoma chemoresistance

    Directory of Open Access Journals (Sweden)

    Duncan Ayers

    2015-01-01

    Conclusions: Based on the initial miRNA findings, this study elucidates the dys-regulation of four miRNAs in three separate NB chemoresistant cell line models, spanning two cell lines (SH-SY5Y and UKF-NB-3 and two chemotherapeutic agents (doxorubicin and etoposide. These miRNAs may thus be possibly linked to chemoresistance induction in NB. Such miRNAs are good candidates to be novel drug targets for future miRNA based therapies against aggressive tumours that are not responding to conventional chemotherapy.

  13. miR-10 in development and cancer

    DEFF Research Database (Denmark)

    Lund, Anders Henrik

    2010-01-01

    The microRNA (miRNA) miR-10 family has attracted attention because of its conservation and the position of the miR-10 genes within the Hox clusters of developmental regulators. In several species, miR-10 is coexpressed with a set of Hox genes and has been found to regulate the translation of Hox ...... function to the miRNA repertoire.Cell Death and Differentiation advance online publication, 22 May 2009; doi:10.1038/cdd.2009.58....

  14. Epigenetic architecture and miRNA: reciprocal regulators

    DEFF Research Database (Denmark)

    Wiklund, Erik Digman; Kjems, Jørgen; Clark, Susan

    2010-01-01

    Deregulation of epigenetic and microRNA (miRNA) pathways are emerging as key events in carcinogenesis. miRNA genes can be epigenetically regulated and miRNAs can themselves repress key enzymes that drive epigenetic remodeling. Epigenetic and miRNA functions are thus tightly interconnected......RNAs) are considered especially promising in clinical applications, and their biogenesis and function is a subject of active research. In this review, the current status of epigenetic miRNA regulation is summarized and future therapeutic prospects in the field are discussed with a focus on cancer....

  15. Epigenetic architecture and miRNA: reciprocal regulators

    DEFF Research Database (Denmark)

    Wiklund, Erik Digman; Kjems, Jørgen; Clark, Susan

    2010-01-01

    Deregulation of epigenetic and microRNA (miRNA) pathways are emerging as key events in carcinogenesis. miRNA genes can be epigenetically regulated and miRNAs can themselves repress key enzymes that drive epigenetic remodeling. Epigenetic and miRNA functions are thus tightly interconnected......RNAs) are considered especially promising in clinical applications, and their biogenesis and function is a subject of active research. In this review, the current status of epigenetic miRNA regulation is summarized and future therapeutic prospects in the field are discussed with a focus on cancer....

  16. Ground-Water Quality in the St. Lawrence River Basin, New York, 2005-06

    Science.gov (United States)

    Nystrom, Elizabeth A.

    2007-01-01

    The Federal Clean Water Act requires that States monitor and report on the quality of ground water and surface water. To satisfy part of these requirements, the U.S. Geological Survey and New York State Department of Environmental Conservation have developed a program in which ground-water quality is assessed in 2 to 3 of New York State's 14 major river basins each year. To characterize the quality of ground water in the St. Lawrence River Basin in northern New York, water samples were collected from 14 domestic and 11 production wells between August 2005 and January 2006. Eight of the wells were finished in sand and gravel and 17 wells were finished in bedrock. Ground-water samples were collected and processed using standard U.S. Geological Survey procedures and were analyzed for 229 constituents and physical properties, including inorganic constituents, nutrients, trace elements, radon-222, pesticides and pesticide degradates, volatile organic compounds, and bacteria. Sixty-six constituents were detected above laboratory reporting levels. Concentrations of most compounds at most sites were within drinking water standards established by the U.S. Environmental Protection Agency and New York State Department of Health, but a few compounds exceeded drinking water standards at some sites. Water in the basin is generally hard to very hard (hardness equal to 121 mg/L as CaCO3 or greater); hardness and alkalinity were generally higher in the St. Lawrence Valley than in the Adirondack Mountains. The cation with the highest median concentration was calcium; the anion with the highest median concentration was bicarbonate. The concentration of chloride in one sample exceeded the 250 milligrams per liter U.S. Environmental Protection Agency Secondary Drinking Water Standard; the concentration of sulfate in one sample also exceeded the 250 milligrams per liter U.S. Environmental Protection Agency Secondary Drinking Water Standard. Nitrate was the predominant nutrient detected

  17. Institute of Geophysics and Planetary Physics, Lawrence Livermore National Laboratory, 1996 Annual Report

    Energy Technology Data Exchange (ETDEWEB)

    Ryerson, F. J., Institute of Geophysics and Planetary Physics

    1998-03-23

    The Institute of Geophysics and Planetary Physics (IGPP) is a Multicampus Research Unit of the University of California (UC). IGPP was founded in 1946 at UC Los Angeles with a charter to further research in the earth and planetary sciences and in related fields. The Institute now has branches at UC campuses in Los Angeles, San Diego, and Riverside, and at Los Alamos and Lawrence Livermore national laboratories. The University-wide IGPP has played an important role in establishing interdisciplinary research in the earth and planetary sciences. For example, IGPP was instrumental in founding the fields of physical oceanography and space physics, which at the time fell between the cracks of established university departments. Because of its multicampus orientation, IGPP has sponsored important interinstitutional consortia in the earth and planetary sciences. Each of the five branches has a somewhat different intellectual emphasis as a result of the interplay between strengths of campus departments and Laboratory programs. The IGPP branch at Lawrence Livermore National Laboratory (LLNL) was approved by the Regents of the University of California in 1982. IGPP-LLNL emphasizes research in seismology, geochemistry, cosmochemistry, and astrophysics. It provides a venue for studying the fundamental aspects of these fields, thereby complementing LLNL programs that pursue applications of these disciplines in national security and energy research. IGPP-LLNL is directed by Charles Alcock and was originally organized into three centers: Geosciences, stressing seismology; High-Pressure Physics, stressing experiments using the two-stage light-gas gun at LLNL; and Astrophysics, stressing theoretical and computational astrophysics. In 1994, the activities of the Center for High-Pressure Physics were merged with those of the Center for Geosciences. The Center for Geosciences, headed by Frederick Ryerson, focuses on research in geophysics and geochemistry. The Astrophysics Research

  18. Institute of Geophysics and Planetary Physics, Lawrence Livermore National Laboratory, 1996 Annual Report

    Energy Technology Data Exchange (ETDEWEB)

    Ryerson, F. J., Institute of Geophysics and Planetary Physics

    1998-03-23

    The Institute of Geophysics and Planetary Physics (IGPP) is a Multicampus Research Unit of the University of California (UC). IGPP was founded in 1946 at UC Los Angeles with a charter to further research in the earth and planetary sciences and in related fields. The Institute now has branches at UC campuses in Los Angeles, San Diego, and Riverside, and at Los Alamos and Lawrence Livermore national laboratories. The University-wide IGPP has played an important role in establishing interdisciplinary research in the earth and planetary sciences. For example, IGPP was instrumental in founding the fields of physical oceanography and space physics, which at the time fell between the cracks of established university departments. Because of its multicampus orientation, IGPP has sponsored important interinstitutional consortia in the earth and planetary sciences. Each of the five branches has a somewhat different intellectual emphasis as a result of the interplay between strengths of campus departments and Laboratory programs. The IGPP branch at Lawrence Livermore National Laboratory (LLNL) was approved by the Regents of the University of California in 1982. IGPP-LLNL emphasizes research in seismology, geochemistry, cosmochemistry, and astrophysics. It provides a venue for studying the fundamental aspects of these fields, thereby complementing LLNL programs that pursue applications of these disciplines in national security and energy research. IGPP-LLNL is directed by Charles Alcock and was originally organized into three centers: Geosciences, stressing seismology; High-Pressure Physics, stressing experiments using the two-stage light-gas gun at LLNL; and Astrophysics, stressing theoretical and computational astrophysics. In 1994, the activities of the Center for High-Pressure Physics were merged with those of the Center for Geosciences. The Center for Geosciences, headed by Frederick Ryerson, focuses on research in geophysics and geochemistry. The Astrophysics Research

  19. Possible effects of changes in CIL temperature and thickness on population dynamics of snow crab, Chionoecetes opilio, in the Gulf of Saint Lawrence

    National Research Council Canada - National Science Library

    Sainte-Marie, B; Gilbert, D

    1998-01-01

    The cold intermediate layer (CIL) of the Gulf of St. Lawrence is subject to interannual changes in core temperature and thickness, which are superimposed on persistent west-east and north-south gradients for these same variables...

  20. miR-21, miR-221 and miR-222 expression and prostate cancer recurrence among obese and non-obese cases

    Institute of Scientific and Technical Information of China (English)

    Ernest K Amankwah; Evelyn Anegbe; Hyun Park; Julio Pow-Sang; Ardeshir Hakam; Jong Y Park

    2013-01-01

    Recent evidence shows that certain microRNAs (miRNAs) play a role in both obesity and prostate cancer recurrence,but the association between the expression of these miRNAs and obesity in prostate cancer recurrence is unknown.In this study,we examined the effect of the interaction between obesity and miR-21,miR-221 or miR-222 expression on prostate cancer recurrence among 28 recurrent and 37 non-recurrent prostate cancer cases,miRNA expression was determined using quantitative real-time polymerase chain reaction.Cox proportional hazard models adjusting for age at diagnosis,clinical stage and Gleason score were used to estimate hazard ratios (HR) and 95% confidence intervals (95% CI) for recurrence free survival.A significantly (P=0.014) higher proportion of recurrent cases (78.6%) than non-recurrent cases (48.6%) had a low expression of miR-21 and the difference was more prominent in obese than non-obese patients.Multivariate analysis showed that the expression of miR-21 was an independent risk factor for recurrence in obese (HR=6.15,95% CI=1.04-36.48,P=0.045),but not in non-obese (HR=1.28,95% CI=0.30-5.49,P=0.74) cases.A significant association with recurrence was not observed for the expression of miR-221 and miR-222.In summary,our findings show that miR-21 is associated with prostate cancer recurrence after radical prostatectomy and suggest that the differential expression of miR-21 is more prominent in obese than in non-obese cases.Future larger studies are warranted to confirm these initial findings and to elucidate the mechanisms involved.

  1. miRNA control of tissue repair and regeneration.

    Science.gov (United States)

    Sen, Chandan K; Ghatak, Subhadip

    2015-10-01

    Tissue repair and regeneration rely on the function of miRNA, molecular silencers that enact post-transcriptional gene silencing of coding genes. Disruption of miRNA homeostasis is developmentally lethal, indicating that fetal tissue development is tightly controlled by miRNAs. Multiple critical facets of adult tissue repair are subject to control by miRNAs, as well. Sources of cell pool for tissue repair and regeneration are diverse and provided by processes including cellular dedifferentiation, transdifferentiation, and reprogramming. Each of these processes is regulated by miRNAs. Furthermore, induced pluripotency may be achieved by miRNA-based strategies independent of transcription factor manipulation. The observation that miRNA does not integrate into the genome makes miRNA-based therapeutic strategies translationally valuable. Tools to manipulate cellular and tissue miRNA levels include mimics and inhibitors that may be specifically targeted to cells of interest at the injury site. Here, we discuss the extraordinary importance of miRNAs in tissue repair and regeneration based on emergent reports and rapid advances in miRNA-based therapeutics.

  2. Microprocessor activity controls differential miRNA biogenesis In Vivo.

    Science.gov (United States)

    Conrad, Thomas; Marsico, Annalisa; Gehre, Maja; Orom, Ulf Andersson

    2014-10-23

    In miRNA biogenesis, pri-miRNA transcripts are converted into pre-miRNA hairpins. The in vivo properties of this process remain enigmatic. Here, we determine in vivo transcriptome-wide pri-miRNA processing using next-generation sequencing of chromatin-associated pri-miRNAs. We identify a distinctive Microprocessor signature in the transcriptome profile from which efficiency of the endogenous processing event can be accurately quantified. This analysis reveals differential susceptibility to Microprocessor cleavage as a key regulatory step in miRNA biogenesis. Processing is highly variable among pri-miRNAs and a better predictor of miRNA abundance than primary transcription itself. Processing is also largely stable across three cell lines, suggesting a major contribution of sequence determinants. On the basis of differential processing efficiencies, we define functionality for short sequence features adjacent to the pre-miRNA hairpin. In conclusion, we identify Microprocessor as the main hub for diversified miRNA output and suggest a role for uncoupling miRNA biogenesis from host gene expression.

  3. Entropy-based model for miRNA isoform analysis.

    Directory of Open Access Journals (Sweden)

    Shengqin Wang

    Full Text Available MiRNAs have been widely studied due to their important post-transcriptional regulatory roles in gene expression. Many reports have demonstrated the evidence of miRNA isoform products (isomiRs in high-throughput small RNA sequencing data. However, the biological function involved in these molecules is still not well investigated. Here, we developed a Shannon entropy-based model to estimate isomiR expression profiles of high-throughput small RNA sequencing data extracted from miRBase webserver. By using the Kolmogorov-Smirnov statistical test (KS test, we demonstrated that the 5p and 3p miRNAs present more variants than the single arm miRNAs. We also found that the isomiR variant, except the 3' isomiR variant, is strongly correlated with Minimum Free Energy (MFE of pre-miRNA, suggesting the intrinsic feature of pre-miRNA should be one of the important factors for the miRNA regulation. The functional enrichment analysis showed that the miRNAs with high variation, particularly the 5' end variation, are enriched in a set of critical functions, supporting these molecules should not be randomly produced. Our results provide a probabilistic framework for miRNA isoforms analysis, and give functional insights into pre-miRNA processing.

  4. Exploration of miRNA families for hypotheses generation.

    KAUST Repository

    Kamanu, T.K.

    2013-10-15

    Technological improvements have resulted in increased discovery of new microRNAs (miRNAs) and refinement and enrichment of existing miRNA families. miRNA families are important because they suggest a common sequence or structure configuration in sets of genes that hint to a shared function. Exploratory tools to enhance investigation of characteristics of miRNA families and the functions of family-specific miRNA genes are lacking. We have developed, miRNAVISA, a user-friendly web-based tool that allows customized interrogation and comparisons of miRNA families for hypotheses generation, and comparison of per-species chromosomal distribution of miRNA genes in different families. This study illustrates hypothesis generation using miRNAVISA in seven species. Our results unveil a subclass of miRNAs that may be regulated by genomic imprinting, and also suggest that some miRNA families may be species-specific, as well as chromosome- and/or strand-specific.

  5. Neuronal Activity Regulates Hippocampal miRNA Expression

    Science.gov (United States)

    Eacker, Stephen M.; Keuss, Matthew J.; Berezikov, Eugene; Dawson, Valina L.; Dawson, Ted M.

    2011-01-01

    Neuronal activity regulates a broad range of processes in the hippocampus, including the precise regulation of translation. Disruptions in proper translational control in the nervous system are associated with a variety of disorders that fall in the autistic spectrum. MicroRNA (miRNA) represent a relatively recently discovered player in the regulation of translation in the nervous system. We have conducted an in depth analysis of how neuronal activity regulates miRNA expression in the hippocampus. Using deep sequencing we exhaustively identify all miRNAs, including 15 novel miRNAs, expressed in hippocampus of the adult mouse. We identified 119 miRNAs documented in miRBase but less than half of these miRNA were expressed at a level greater than 0.1% of total miRNA. Expression profiling following induction of neuronal activity by electroconvulsive shock demonstrates that most miRNA show a biphasic pattern of expression: rapid induction of specific mature miRNA expression followed by a decline in expression. These results have important implications into how miRNAs influence activity-dependent translational control. PMID:21984899

  6. Neuronal activity regulates hippocampal miRNA expression.

    Directory of Open Access Journals (Sweden)

    Stephen M Eacker

    Full Text Available Neuronal activity regulates a broad range of processes in the hippocampus, including the precise regulation of translation. Disruptions in proper translational control in the nervous system are associated with a variety of disorders that fall in the autistic spectrum. MicroRNA (miRNA represent a relatively recently discovered player in the regulation of translation in the nervous system. We have conducted an in depth analysis of how neuronal activity regulates miRNA expression in the hippocampus. Using deep sequencing we exhaustively identify all miRNAs, including 15 novel miRNAs, expressed in hippocampus of the adult mouse. We identified 119 miRNAs documented in miRBase but less than half of these miRNA were expressed at a level greater than 0.1% of total miRNA. Expression profiling following induction of neuronal activity by electroconvulsive shock demonstrates that most miRNA show a biphasic pattern of expression: rapid induction of specific mature miRNA expression followed by a decline in expression. These results have important implications into how miRNAs influence activity-dependent translational control.

  7. Exosomal miRNAs as cancer biomarkers and therapeutic targets

    Directory of Open Access Journals (Sweden)

    Arron Thind

    2016-07-01

    Full Text Available Intercommunication between cancer cells and with their surrounding and distant environments is key to the survival, progression and metastasis of the tumour. Exosomes play a role in this communication process. MicroRNA (miRNA expression is frequently dysregulated in tumour cells and can be reflected by distinct exosomal miRNA (ex-miRNA profiles isolated from the bodily fluids of cancer patients. Here, the potential of ex-miRNA as a cancer biomarker and therapeutic target is critically analysed. Exosomes are a stable source of miRNA in bodily fluids but, despite a number of methods for exosome extraction and miRNA quantification, their suitability for diagnostics in a clinical setting is questionable. Furthermore, exosomally transferred miRNAs can alter the behaviour of recipient tumour and stromal cells to promote oncogenesis, highlighting a role in cell communication in cancer. However, our incomplete understanding of exosome biogenesis and miRNA loading mechanisms means that strategies to target exosomes or their transferred miRNAs are limited and not specific to tumour cells. Therefore, if ex-miRNA is to be employed in novel non-invasive diagnostic approaches and as a therapeutic target in cancer, two further advances are necessary: in methods to isolate and detect ex-miRNA, and a better understanding of their biogenesis and functions in tumour-cell communication.

  8. Seasonal variability of denitrification efficiency in northern salt marshes: an example from the St. Lawrence Estuary.

    Science.gov (United States)

    Poulin, Patrick; Pelletier, Emilien; Saint-Louis, Richard

    2007-06-01

    In coastal ecosystems, denitrification is a key process in removing excess dissolved nitrogen oxides and participating in the control of eutrophication process. Little is known about the role of salt marshes on nitrogen budgets in cold weather coastal areas. Although coastal salt marshes are important sites for organic matter degradation and nutrient regeneration, bacterial-mediated nitrogen cycling processes, such as denitrification, remain unknown in northern and sub-arctic regions, especially under winter conditions. Using labelled nitrogen (15N), denitrification rates were measured in an eastern Canadian salt marsh in August, October and December 2005. Freshly sampled undisturbed sediment cores were incubated over 8h and maintained at their sampling temperatures to evaluate the influence of low temperatures on the denitrification rate. From 2 to 12 degrees C, average denitrification rate and dissolved oxygen consumption increased from 9.6 to 25.5 micromol N2 m-2 h-1 and from 1.3 to 1.8 mmol O2 m-2 h-1, respectively, with no statistical dependence of temperature (p>0.05). Nitrification has been identified as the major nitrate source for denitrification, supplying more than 80% of the nitrate demand. Because no more than 31% of the nitrate removed by sediment is estimated to be denitrified, the presence of a major nitrate sink in sediment is suspected. Among possible nitrate consumption mechanisms, dissimilatory reduction of nitrate to ammonium, metal and organic matter oxidation processes are discussed. Providing the first measurements of denitrification rate in a St. Lawrence Estuary salt marsh, this study evidences the necessity of preserving and restoring marshes. They constitute an efficient geochemical filter against an excess of nitrate dispersion to coastal waters even under cold northern conditions.

  9. Molecular and spectroscopic analysis of non-hydrolyzable sedimentary organic matter from the St. Lawrence Estuary

    Science.gov (United States)

    Ibrahim, M.; Simpson, A. J.; Gelinas, Y.

    2011-12-01

    Sediments are the ultimate long-term sink for organic carbon (OC) on Earth, thus playing an important role in the global cycles of O2 and CO2. Estuaries and river deltas are major conduits for terrestrial organic matter (OM) into marine systems, where it is mixed with locally produced OM and is eventually deposited and buried in the sediment bed. About 45% of global OC burial occurs along these river deltas and estuaries (Hedges and Keil, 1995), therefore it is of interest to follow OM deposition and preservation in these terrestrial to marine transition zones. We chemically fractionated bulk OM from a series of sediments from the St. Lawrence Estuary and Gulf into distinct reactivity classes. We define three such OM fractions based on pioneering work by Hedges and Keil (1995) who classified OM based on chemical reactivities: labile (degradable at similar rate under oxic and anoxic conditions), non-hydrolyzable (degraded primarily under oxic conditions), and refractory (preserved on long time scales independently of redox conditions). Here we present data on the elemental (C and N), isotopic (δ13C and δ15N) and spectroscopic (FTIR, HR-MAS 1H/13C NMR) characterization of the different fractions, focusing primarily on the non-hydrolyzable fraction because of its importance in the long-term burial of OC below the seafloor. We used mild oxidation methods (cupric oxide and ruthenium tetroxide oxidation) followed by chromatographic analysis of the oxidation products. Combining results from the bulk and molecular analytical techniques provides insights into the composition and cycling of non-hydrolyzable OM in transitional system, from which OM preservation can be better understood. Hedges, J. I., Keil, Richard G. (1995). "Sedimentary organic matter preservation: an assessment and speculative synthesis." Marine Chemistry 49(2-3): 81-115.

  10. Progress in safety and environmental aspects of inertial fusion energy at Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Latkowski, J F; Reyes, S; Meier, W R

    2000-06-01

    Lawrence Livermore National Laboratory (LLNL) is making significant progress in several areas related to the safety and environmental (S and E) aspects of inertial fusion energy (IFE). A detailed accident analysis has been completed for the HYLIFE-II power plant design. Additional accident analyses are underway for both the HYLIFE-II and Sombrero designs. Other S and E work at LLNL has addressed the issue of the driver-chamber interface and its importance for both heavy-ion and laser-driven IFE. Radiation doses and fluences have been calculated for final focusing mirrors and magnets and shielding optimization is underway to extend the anticipated lifetimes for key components. Target designers/fabrication specialists have been provided with ranking information related to the S and E characteristics of candidate target materials (e.g., ability to recycle, accident consequences, and waste management). Ongoing work in this area will help guide research directions and the selection of target materials. Published and continuing work on fast ignition has demonstrated some of the potentially attractive S and E features of such designs. In addition to reducing total driver energies, fast ignition may ease target fabrication requirements, reduce radiation damage rates, and enable the practical use of advanced (e.g., tritium-lean) labels with significantly reduced neutron production rates, the possibility of self-breeding targets, and dramatically increased flexibility in blanket design. Domestic and international collaborations are key to success in the above areas. A brief summary of each area is given and plans for future work are outlined.

  11. Environmental impact report addendum for the continued operation of Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Weston, R. F. [Roy F. Weston, Inc. (United States)

    1996-10-01

    An environmental impact statement/environmental impact report (ES/EIR) for the continued operation and management of Lawrence Livermore National Laboratory (LLNL) was prepared jointly by the U.S. Department of Energy (DOE) and the University of California (UC). The scope of the document included near-term (within 5-10 years) proposed projects. The UC Board of Regents, as state lead agency under the California Environmental Quality Act (CEQA), certified and adopted the EIR by issuing a Notice of Determination on November 20, 1992. The DOE, as the lead federal agency under the National Environmental Policy Act (NEPA), adopted a Record of Decision for the ES on January 27, 1993 (58 Federal Register [FR] 6268). The DOE proposed action was to continue operation of the facility, including near-term proposed projects. The specific project evaluated by UC was extension of the contract between UC and DOE for UC`s continued operation and management of LLNL (both sites) from October 1, 1992, through September 30, 1997. The 1992 ES/EIR analyzed impacts through the year 2002. The 1992 ES/EIR comprehensively evaluated the potential environmental impacts of operation and management of LLNL within the near-term future. Activities evaluated included programmatic enhancements and modifications of facilities and programs at the LLNL Livermore site and at LLNL`s Experimental Test Site (Site 300) in support of research and development missions 2048 established for LLNL by Congress and the President. The evaluation also considered the impacts of infrastructure and building maintenance, minor modifications to buildings, general landscaping, road maintenance, and similar routine support activities.

  12. An Alternative Paradigm for Evidence-Based Medicine: Revisiting Lawrence Weed, MD’s Systems Approach

    Science.gov (United States)

    Shukor, Ali Rafik

    2017-01-01

    Lawrence Weed, MD, is renowned for being the father of the Problem-Oriented Medical Record (POMR), the medical care standard for collecting, managing, and contextualizing patient data in medical records. What have been consistently overlooked are his teachings on knowledge coupling, which refers to matching patient data with associated medical knowledge. Together, the POMR standard and knowledge coupling are meant to form the basis of a systems approach that enables individualized evidence-based decision making within the context of multimorbidity and patient complexity. The POMR and knowledge coupling tools operationalize a problem-oriented model that reflects a sophisticated general systems theoretical approach to knowledge. This paradigm transcends reductionist approaches to knowledge by depicting how the meaning of specific entities (eg, disease constructs) and their associated probabilities can only be understood within their respective spatiotemporal and biopsychosocial relational contexts. Rigorous POMRs therefore require knowledge inputs from a network of interconnections among specific entities, which Dr Weed enabled through development of the Knowledge Net standard. The Knowledge Net’s relational structure determines the applicability of knowledge within specific patient contexts. To enable the linkage of unique combinations of data in individual patient POMRs with existing medical knowledge structured in Knowledge Nets, Dr Weed developed the Knowledge Coupling standard. Dr Weed’s standards for record keeping and knowledge coupling form the basis of a combinatorial approach to evidence-based medicine that fulfills Stange’s call for a science of connectedness. Ensuing individualized processes of care become the dynamo powering a learning health care system that enables a co-construction of health premised on empowerment and intelligent human decision making, rather than promoting the artificial intelligence of tools. If the value of Engel

  13. Visibility of St Lawrence belugas to aerial photography, estimated by direct observation

    Directory of Open Access Journals (Sweden)

    Michael CS Kingsley

    2002-07-01

    Full Text Available The depleted population of belugas (Delphinapterus leucas inhabiting the St Lawrence estuary, Canada, was monitored by periodic photographic aerial surveys. In order to correct counts made on aerial survey film and to obtain an estimate of the true size of the population, the diving behaviour and the visibility from the air of these animals was studied. A Secchi-disk turbidity survey in the belugas’ summer range showed that water clarity varied between 1.5 m and 11.6 m. By studying aerial photographs of sheet-plastic models of belugas that had been sunk to different depths below the surface, we found that models of white adults could be seen down to about the same depth as a Secchi disk, but no deeper. Smaller models of dark-grey juveniles could only be seen down to about 50% of Secchi-disk depth. By observing groups of belugas from a hovering helicopter and recording their disappearances and re-appearances, it was found that they were visible for 44.3% of the time, and that an appropriate correction for single photographs would be to multiply the photographic count by about 222% (SE 20%. For surveys in which there was overlap between adjacent frames, the estimated correction would be 209% (SE 16%. This correction factor was slightly conservative and gave an estimate of the true size of the population, based on a single survey, of 1,202 belugas (SE 189 in 1997. An estimate for 1997 based on smoothing 5 surveys 1988–1997 was 1,238 (SE 119.

  14. Hydrogeology and tritium transport in Chicken Creek Canyon,Lawrence Berkeley National Laboratory, Berkeley, California

    Energy Technology Data Exchange (ETDEWEB)

    Jordan, Preston D.; Javandel, Iraj

    2007-10-31

    This study of the hydrogeology of Chicken Creek Canyon wasconducted by the Environmental Restoration Program (ERP) at LawrenceBerkeley National Laboratory (LBNL). This canyon extends downhill fromBuilding 31 at LBNL to Centennial Road below. The leading edge of agroundwater tritium plume at LBNL is located at the top of the canyon.Tritium activities measured in this portion of the plume during thisstudy were approximately 3,000 picocuries/liter (pCi/L), which issignificantly less than the maximum contaminant level (MCL) for drinkingwaterof 20,000 pCi/L established by the Environmental ProtectionAgency.There are three main pathways for tritium migration beyond theLaboratory s boundary: air, surface water and groundwater flow. Thepurpose of this report is to evaluate the groundwater pathway.Hydrogeologic investigation commenced with review of historicalgeotechnical reports including 35 bore logs and 27 test pit/trench logsas well as existing ERP information from 9 bore logs. This was followedby field mapping of bedrock outcrops along Chicken Creek as well asbedrock exposures in road cuts on the north and east walls of the canyon.Water levels and tritium activities from 6 wells were also considered.Electrical-resistivity profiles and cone penetration test (CPT) data werecollected to investigate the extent of an interpreted alluvial sandencountered in one of the wells drilled in this area. Subsequent loggingof 7 additional borings indicated that this sand was actually anunusually well-sorted and typically deeply weathered sandstone of theOrinda Formation. Wells were installed in 6 of the new borings to allowwater level measurement and analysis of groundwater tritium activity. Aslug test and pumping tests were also performed in the wellfield.

  15. Environmental assessment for the Explosive Waste Treatment Facility at Site 300, Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-11-01

    Lawrence Livermore National Laboratory proposes to build, permit, and operate the Explosive Waste Treatment Facility (EWTF) to treat explosive waste at LLNL`s Experimental Test Site, Site 300. It is also proposed to close the EWTF at the end of its useful life in accordance with the regulations. The facility would replace the existing Building 829 Open Burn Facility (B829) and would treat explosive waste generated at the LLNL Livermore Site and at Site 300 either by open burning or open detonation, depending on the type of waste. The alternatives addressed in the 1992 sitewide EIS/EIR are reexamined in this EA. These alternatives included: (1) the no-action alternative which would continue open burning operations at B829; (2) continuation of only open burning at a new facility (no open detonation); (3) termination of open burning operations with shipment of explosive waste offsite; and (4) the application of alternative treatment technologies. This EA examines the impact of construction, operation, and closure of the EWTF. Construction of the EWTF would result in the clearing of a small amount of previously disturbed ground. No adverse impact is expected to any state or federal special status plant or animal species (special status species are classified as threatened, endangered, or candidate species by either state or federal legislation). Operation of the EWTF is expected to result in a reduced threat to involved workers and the public because the proposed facility would relocate existing open burning operations to a more remote area and would incorporate design features to reduce the amount of potentially harmful emissions. No adverse impacts were identified for activities necessary to close the EWTF at the end of its useful life.

  16. Public census data on CD-ROM at Lawrence Berkeley Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Merrill, D.W.

    1993-01-16

    The Comprehensive Epidemiologic Data Resource (CEDR) and Populations at Risk to Environmental Pollution (PAREP) projects, of the Information and Computing Sciences Division (ICSD) at Lawrence Berkeley Laboratory (LBL), are using public socioeconomic and geographic data files which are available to CEDR and PAREP collaborators via LBL's computing network. At this time 72 CD-ROM diskettes (approximately 37 gigabytes) are on line via the Unix file server cedrcd.lbl.gov''. Most of the files are from the US Bureau of the Census, and many of these pertain to the 1990 Census of Population and Housing. All the CD-ROM diskettes contain documentation in the form of ASCII text files. In addition, printed documentation for most files is available for inspection at University of California Data and Technical Assistance (UC DATA), tel. (510) 642-6571, or the UC Documents Library, tel. (510) 642-2569, both located on the UC Berkeley Campus. Many of the CD-ROM diskettes distributed by the Census Bureau contain software for PC compatible computers, for easily accessing the data. Shared access to the data is maintained through a collaboration among the CEDR and PAREP projects at LBL, and UC DATA, and the UC Documents Library. LBL is grateful to UC DATA and the UC Documents Library for the use of their CD-ROM diskettes. Shared access to LBL facilities may be restricted in the future if costs become prohibitive. Via the Sun Network File System (NFS), these data can be exported to Internet computers for direct access by the user's application program(s). Due to the size of the files, this access method is preferred over File Transfer Protocol (FTP) access. Please contact Deane Merrill (dwmerrill lbl.gov) if you wish to make use of the data.

  17. Public census data on CD-ROM at Lawrence Berkeley Laboratory. Revision 4

    Energy Technology Data Exchange (ETDEWEB)

    Merrill, D.W.

    1993-03-12

    The Comprehensive Epidemiologic Data Resource (CEDR) and Populations at Risk to Environmental Pollution (PAREP) projects, of the Information and Computing sciences Division (ICSD) at Lawrence Berkeley Laboratory (LBL), are using public socioeconomic and geographic data files which are available to CEDR and PAREP collaborators via LBL`s computing network. At this time 89 CD-ROM diskettes (approximately 45 gigabytes) are on line via the Unix file server cedrcd.lbl.gov. Most of the files are from the US Bureau of the Census, and many of these pertain to the 1990 Census of Population and Housing. All the CD-ROM diskettes contain documentation in the form of ASCII text files. In addition, printed documentation for most files is available for inspection at University of California Data and Technical Assistance (UC DATA), tel. (510) 642-6571, or the UC Documents Library, tel. (510) 642-2569, both located on the UC Berkeley Campus. Many of the CD-ROM diskettes distributed by the Census Bureau contain software for PC compatible computers, for easily accessing the data. Shared access to the data is maintained through a collaboration among the CEDR and PAREP projects at LBL, and UC DATA, and the UC Documents Library. LBL is grateful to UC DATA and the UC Documents Library for the use of their CD-ROM diskettes. Shared access to LBL facilities may be restricted in the future if costs become prohibitive. Via the Sun Network File System (NFS), these data can be exported to Internet computers for direct access by the user`s application program(s). Due to the size of the files, this access method is preferred over File Transfer Protocol (FTP) access.

  18. Public census data on CD-ROM at Lawrence Berkeley Laboratory. Revision 3

    Energy Technology Data Exchange (ETDEWEB)

    Merrill, D.W.

    1993-01-16

    The Comprehensive Epidemiologic Data Resource (CEDR) and Populations at Risk to Environmental Pollution (PAREP) projects, of the Information and Computing Sciences Division (ICSD) at Lawrence Berkeley Laboratory (LBL), are using public socioeconomic and geographic data files which are available to CEDR and PAREP collaborators via LBL`s computing network. At this time 72 CD-ROM diskettes (approximately 37 gigabytes) are on line via the Unix file server ``cedrcd.lbl.gov``. Most of the files are from the US Bureau of the Census, and many of these pertain to the 1990 Census of Population and Housing. All the CD-ROM diskettes contain documentation in the form of ASCII text files. In addition, printed documentation for most files is available for inspection at University of California Data and Technical Assistance (UC DATA), tel. (510) 642-6571, or the UC Documents Library, tel. (510) 642-2569, both located on the UC Berkeley Campus. Many of the CD-ROM diskettes distributed by the Census Bureau contain software for PC compatible computers, for easily accessing the data. Shared access to the data is maintained through a collaboration among the CEDR and PAREP projects at LBL, and UC DATA, and the UC Documents Library. LBL is grateful to UC DATA and the UC Documents Library for the use of their CD-ROM diskettes. Shared access to LBL facilities may be restricted in the future if costs become prohibitive. Via the Sun Network File System (NFS), these data can be exported to Internet computers for direct access by the user`s application program(s). Due to the size of the files, this access method is preferred over File Transfer Protocol (FTP) access. Please contact Deane Merrill (dwmerrill@lbl.gov) if you wish to make use of the data.

  19. Lawrence Livermore National Laboratory offsite hazardous waste shipment data validation report

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-09-01

    The U.S. Department of Energy Headquarters requested this report to verify that Lawrence Livermore National Laboratory (LLNL) properly categorized hazardous waste shipped offsite from 1984 to 1991. LLNL categorized the waste shipments by the new guidelines provided on the definition of radioactive waste. For this validation, waste that has had no radioactivity added by DOE operations is nonradioactive. Waste to which DOE operations has added or concentrated any radioactivity is radioactive. This report documents findings from the review of available LLNL hazardous waste shipment information and summarizes the data validation strategy. The report discusses administrative and radiological control procedures in place at LLNL during the data validation period. It also describes sampling and analysis and surface survey procedures used in determining radionuclide concentrations for offsite release of hazardous waste shipments. The evaluation team reviewed individual items on offsite hazardous waste shipments and classified them, using the DOE-HQ waste category definitions. LLNL relied primarily on generator knowledge to classify wastes. Very little radioanalytical information exists on hazardous wastes shipped from LLNL. Slightly greater than one-half of those hazardous waste items for which the documentation included radioanalytical data showed concentrations of radioactivity higher than the LLNL release criteria used from 1989 to 1991. Based on this small amount of available radioanalytical data, very little (less than one percent) of the hazardous waste generated at the LLNL main site can be shown to contain DOE added radioactivity. LLNL based the criteria on the limit of analytical sensitivity for gross alpha and gross beta measurements and the background levels of tritium. Findings in this report are based on information and documentation on the waste handling procedures in place before the start of the hazardous waste shipping moratorium in May 1991.

  20. Oleic Acid Induces MiR-7 Processing through Remodeling of Pri-MiR-7/Protein Complex.

    Science.gov (United States)

    Kumar, Santosh; Downie Ruiz Velasco, Angela; Michlewski, Gracjan

    2017-06-02

    MicroRNAs (miRs) play a vital role in governing cell function, with their levels tightly controlled at transcriptional and post-transcriptional levels. Different sets of RNA-binding proteins interact with primary miRs (pri-miRs) and precursor-miR transcripts (pre-miRs), controlling their biogenesis post-transcriptionally. The Hu antigen R (HuR)-mediated binding of Musashi homolog2 (MSI2) to the conserved terminal loop of pri-miR-7 regulates the levels of brain-enriched miR-7 formation in a tissue-specific manner. Here, we show that oleic acid (OA) inhibits the binding of proteins containing RNA recognition motifs (RRM) to the conserved terminal loop of pri-miR-7. Using electrophoretic mobility shift assays in HeLa cell extracts, we show that OA treatment disrupts pre-miR/protein complexes. Furthermore, OA rescues in vitro processing of pri-miR-7, which is otherwise blocked by HuR and MSI2 proteins. On the contrary, pri-miR-16 shows reduced processing in the presence of OA. This indicates that OA may inhibit the binding of other RRM-containing protein/s necessary for miR-16 processing. Finally, we demonstrate that OA induces mature miR-7 production in HeLa cells. Together, our results demonstrate that OA can regulate the processing of pri-miRs by remodeling their protein complexes. This provides a new tool to study RNA processing and a potential lead for small molecules that target the miR-7 biogenesis pathway. Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.

  1. 冠心病患者血清同型半胱氨酸、miR-1、miR-126和 miR-208的相关性分析%Correlation analysis of Hcy,miR-1,miR-126 and miR-208 in coronary heart disease patients

    Institute of Scientific and Technical Information of China (English)

    金洁; 王俊; 王磊; 蔡晓敏; 王立军; 宫剑滨

    2014-01-01

    Objective To study the correlation between miRNA (miR-1,miR-126,miR-208) and homocysteine (Hcy) con-bining on the severity of stenosis of coronary artery .Methods One hundred and two patients undergoing quantitative coronary angiogra -phy were involved in this study .Patients were categorized into control group and coronary artery disease ( CAD) group.The concentra-tion of Hcy and miRNA (miR-1,miR-126,miR-208) were measured.The severity scale of coronary artery stenosis was quantitatively assessed according to coronary angiography by Gensini score .The correlation analysis and linear regression were used to show the asso-ciation of Hcy,miRNA (miR-1,miR-126 and miR-208) and Gensini score.Results The concentration of Hcy and miRNA (miR-1, miR-126 and miR-208) were higher in CAD group than those in control group .In the correlation analysis ,Gensini score was positively associated with Hcy,miR-1 and miR-126 (r=0.575,0.649,and 0.499,P<0.01) and the positive correlation between them remained significant after revising partial correlation analysis (r'=0.693,0.621,and 0.532,P<0.05).Hcy was influenced by miR-1 and miR-126 both in the Spearson correlation analysis (r=0.513,0.56,P<0.01) and partial correlation analysis (r'=0.599,0.614,P<0.05),but not by miR-208.Conclusion Hcy,miRNA (miR-1,miR-126 and miR-208) are independent risk factor for coronary heart disease and are increased along with stenosis of coronary arteries .%目的:结合冠状动脉狭窄程度,探讨冠心病患者血清同型半胱氨酸( Hcy)与微小RNA( miR-1、miR-126及miR-208)之间的相互关系。方法选取行冠脉造影术的患者共102名,根据造影结果分为对照组和观察组,检测Hcy、miR-1、miR-126及miR-208水平,同时对冠脉造影结果进行评价并计算Gensini积分,应用相关及线性回归分析Hcy、miRNA( miR-1、miR-126、miR-208)以及Gensini积分的相互关系。结果观察组血清Hcy、miRNA(miR-1、miR-126、miR-208)水平均

  2. Temporal Variability of Monthly Daily Extreme Water Levels in the St. Lawrence River at the Sorel Station from 1912 to 2010

    OpenAIRE

    2014-01-01

    Although climate models predict that the impacts of climate change on the temporal variability of water levels in the St. Lawrence River will be seasonally-dependent, such a seasonal effect on the current variability of extreme water levels has never been analyzed. To address this, we analyzed the temporal variability of three hydrological variables (monthly daily maximums and minimums, as well as their ratio) of water levels in the St. Lawrence River measured at the Sorel station since 1912,...

  3. Prognostic significance of miR-205 in endometrial cancer.

    Directory of Open Access Journals (Sweden)

    Mihriban Karaayvaz

    Full Text Available PURPOSE: microRNAs have emerged as key regulators of gene expression, and their altered expression has been associated with tumorigenesis and tumor progression. Thus, microRNAs have potential as both cancer biomarkers and/or potential novel therapeutic targets. Although accumulating evidence suggests the role of aberrant microRNA expression in endometrial carcinogenesis, there are still limited data available about the prognostic significance of microRNAs in endometrial cancer. The goal of this study is to investigate the prognostic value of selected key microRNAs in endometrial cancer by the analysis of archival formalin-fixed paraffin-embedded tissues. EXPERIMENTAL DESIGN: Total RNAs were extracted from 48 paired normal and endometrial tumor specimens using Trizol based approach. The expression of miR-26a, let-7g, miR-21, miR-181b, miR-200c, miR-192, miR-215, miR-200c, and miR-205 were quantified by real time qRT-PCR expression analysis. Targets of the differentially expressed miRNAs were quantified using immunohistochemistry. Statistical analysis was performed by GraphPad Prism 5.0. RESULTS: The expression levels of miR-200c (P<0.0001 and miR-205 (P<0.0001 were significantly increased in endometrial tumors compared to normal tissues. Kaplan-Meier survival analysis revealed that high levels of miR-205 expression were associated with poor patient overall survival (hazard ratio, 0.377; Logrank test, P = 0.028. Furthermore, decreased expression of a miR-205 target PTEN was detected in endometrial cancer tissues compared to normal tissues. CONCLUSION: miR-205 holds a unique potential as a prognostic biomarker in endometrial cancer.

  4. Role of miRNA-9 in Brain Development

    Science.gov (United States)

    Radhakrishnan, Balachandar; Alwin Prem Anand, A.

    2016-01-01

    MicroRNAs (miRNAs) are a class of small regulatory RNAs involved in gene regulation. The regulation is effected by either translational inhibition or transcriptional silencing. In vertebrates, the importance of miRNA in development was discovered from mice and zebrafish dicer knockouts. The miRNA-9 (miR-9) is one of the most highly expressed miRNAs in the early and adult vertebrate brain. It has diverse functions within the developing vertebrate brain. In this article, the role of miR-9 in the developing forebrain (telencephalon and diencephalon), midbrain, hindbrain, and spinal cord of vertebrate species is highlighted. In the forebrain, miR-9 is necessary for the proper development of dorsoventral telencephalon by targeting marker genes expressed in the telencephalon. It regulates proliferation in telencephalon by regulating Foxg1, Pax6, Gsh2, and Meis2 genes. The feedback loop regulation between miR-9 and Nr2e1/Tlx helps in neuronal migration and differentiation. Targeting Foxp1 and Foxp2, and Map1b by miR-9 regulates the radial migration of neurons and axonal development. In the organizers, miR-9 is inversely regulated by hairy1 and Fgf8 to maintain zona limitans interthalamica and midbrain–hindbrain boundary (MHB). It maintains the MHB by inhibiting Fgf signaling genes and is involved in the neurogenesis of the midbrain–hindbrain by regulating Her genes. In the hindbrain, miR-9 modulates progenitor proliferation and differentiation by regulating Her genes and Elav3. In the spinal cord, miR-9 modulates the regulation of Foxp1 and Onecut1 for motor neuron development. In the forebrain, midbrain, and hindbrain, miR-9 is necessary for proper neuronal progenitor maintenance, neurogenesis, and differentiation. In vertebrate brain development, miR-9 is involved in regulating several region-specific genes in a spatiotemporal pattern. PMID:27721656

  5. Evolutionary relationships between miRNA genes and their activity.

    Science.gov (United States)

    Zhu, Yan; Skogerbø, Geir; Ning, Qianqian; Wang, Zhen; Li, Biqing; Yang, Shuang; Sun, Hong; Li, Yixue

    2012-12-22

    The emergence of vertebrates is characterized by a strong increase in miRNA families. MicroRNAs interact broadly with many transcripts, and the evolution of such a system is intriguing. However, evolutionary questions concerning the origin of miRNA genes and their subsequent evolution remain unexplained. In order to systematically understand the evolutionary relationship between miRNAs gene and their function, we classified human known miRNAs into eight groups based on their evolutionary ages estimated by maximum parsimony method. New miRNA genes with new functional sequences accumulated more dynamically in vertebrates than that observed in Drosophila. Different levels of evolutionary selection were observed over miRNA gene sequences with different time of origin. Most genic miRNAs differ from their host genes in time of origin, there is no particular relationship between the age of a miRNA and the age of its host genes, genic miRNAs are mostly younger than the corresponding host genes. MicroRNAs originated over different time-scales are often predicted/verified to target the same or overlapping sets of genes, opening the possibility of substantial functional redundancy among miRNAs of different ages. Higher degree of tissue specificity and lower expression level was found in young miRNAs. Our data showed that compared with protein coding genes, miRNA genes are more dynamic in terms of emergence and decay. Evolution patterns are quite different between miRNAs of different ages. MicroRNAs activity is under tight control with well-regulated expression increased and targeting decreased over time. Our work calls attention to the study of miRNA activity with a consideration of their origin time.

  6. 非小细胞肺癌血浆miR-223、miR-93和miR-218的表达及其临床意义%Expression and clinical value analysis of plasma miR-223, miR-93 and miR-218 in non-small lung cancer

    Institute of Scientific and Technical Information of China (English)

    蔡静清; 王宁; 张敏

    2014-01-01

    Objective To explore the levels of plasma miR⁃223, miR⁃93 and miR⁃218 in non⁃small lung cancer( NSCLC) and analysis the relationship between the 3 indicators and clinicopathological parameters of NSCLC. Methods Plasma samples from 85 NSCLC patients before treatment were collected as NSCLC group. The real⁃time quantitative RT⁃PCR( qRT⁃PCR) was used to detect the levels of miR⁃223, miR⁃93 and miR⁃218 and the clinicopathological parameters of NSCLC patients were collected. The clinicopatho⁃logical parameters of patients with different levels of miR⁃223, miR⁃93 and miR⁃218 were compared. The receiver operating characteris⁃tic curve( ROC) was employed to analysis the clinical value of plasma miR⁃223, miR⁃93 and miR⁃218 in the diagnosis of NSCLC. Meanwhile, the plasma samples from 90 healthy volunteers at the same time were selected as control( control group) . Results There were higher levels of miR⁃223 and miR⁃93 but lower miR⁃218 in NSCLC group compaired with control group with significant difference ( P<0�05) . The level of miR⁃223 was related with TNM stage and tumor size, and miR⁃93 was related with histological type and lymph node metastasis, and miR⁃218 was related with tumor size and degree of differentiation, showing with significant difference ( P all<0�05) . The plasma level of miR⁃223 was positively correlated with miR⁃93( r=0�411) , but miR⁃223 and miR⁃93 were negatively cor⁃related with miR⁃218(r=-0�361 and r=-0�451)with statistically significance(P<0�05). The AUC, sensitivity and specificity of plasma miR⁃223, miR⁃93 and miR⁃218 in the diagnosis of NSCLC were 0�926, 95�2% and 87�1%, 0�912, 88�4% and 92�5%, and 0�941, 92�7% and 84�4%, higher than 0�774, 75�1% and 66�2% of carcinoembryonic antigen( CEA) . The combined efficiency of miR⁃223, miR⁃93 and miR⁃218 in NSCLC was superior to that alone. Conclusion There are higher expression of miR⁃223 and

  7. miRSponge: a manually curated database for experimentally supported miRNA sponges and ceRNAs.

    Science.gov (United States)

    Wang, Peng; Zhi, Hui; Zhang, Yunpeng; Liu, Yue; Zhang, Jizhou; Gao, Yue; Guo, Maoni; Ning, Shangwei; Li, Xia

    2015-01-01

    In this study, we describe miRSponge, a manually curated database, which aims at providing an experimentally supported resource for microRNA (miRNA) sponges. Recent evidence suggests that miRNAs are themselves regulated by competing endogenous RNAs (ceRNAs) or 'miRNA sponges' that contain miRNA binding sites. These competitive molecules can sequester miRNAs to prevent them interacting with their natural targets to play critical roles in various biological and pathological processes. It has become increasingly important to develop a high quality database to record and store ceRNA data to support future studies. To this end, we have established the experimentally supported miRSponge database that contains data on 599 miRNA-sponge interactions and 463 ceRNA relationships from 11 species following manual curating from nearly 1200 published articles. Database classes include endogenously generated molecules including coding genes, pseudogenes, long non-coding RNAs and circular RNAs, along with exogenously introduced molecules including viral RNAs and artificial engineered sponges. Approximately 70% of the interactions were identified experimentally in disease states. miRSponge provides a user-friendly interface for convenient browsing, retrieval and downloading of dataset. A submission page is also included to allow researchers to submit newly validated miRNA sponge data. Database URL: http://www.bio-bigdata.net/miRSponge.

  8. Identification and analysis of miRNAs and their targets in ginger using bioinformatics approach.

    Science.gov (United States)

    Singh, Noopur; Srivastava, Swati; Sharma, Ashok

    2016-01-10

    MicroRNAs (miRNAs) are a large family of endogenous small RNAs derived from the non-protein coding genes. miRNA regulates the gene expression at the post-transcriptional level and plays an important role in plant development. Zingiber officinale is an important medicinal plant having numerous therapeutic properties. Its bioactive compound gingerol and essential oil posses important pharmacological and physiological activities. In this study, we used a homology search based computational approach for identifying miRNAs in Z. officinale. A total of 16 potential miRNA families (miR167, miR407, miR414, miR5015, miR5021, miR5644, miR5645, miR5656, miR5658, miR5664, miR827, miR838, miR847, miR854, miR862 and miR864) were predicted in ginger. Phylogenetic and conserved analyses were performed for predicted miRNAs. Thirteen miRNA families were found to regulate 300 target transcripts and play an important role in cell signaling, reproduction, metabolic process and stress. To understand the miRNA mediated gene regulatory control and to validate miRNA target predictions, a biological network was also constructed. Gene ontology and pathway analyses were also done. miR5015 was observed to regulate the biosynthesis of gingerol by inhibiting phenyl ammonia lyase (PAL), a precursor enzyme in the biosynthesis of gingerol. Our results revealed that most of the predicted miRNAs were involved in the regulation of rhizome development. miR5021, miR854 and miR838 were identified to regulate the rhizome development and the essential oil biosynthesis in ginger. Copyright © 2015 Elsevier B.V. All rights reserved.

  9. Study of miR-125b, miR-29a and miR-155-5p as biomarkers of tuberculous meningitis%miR-125b、miR-29a和miR-155-5p作为诊断结核性脑膜炎生物标志的研究

    Institute of Scientific and Technical Information of China (English)

    柴璐璐; 田宋新; 袁俐; 王宏

    2015-01-01

    Objective To investigate the expression levels of microRNA(miR)-125b, miR-29a and miR-155-5p in ce⁃rebrospinal fluid (CSF) and plasma from the patients with tuberculous meningitis and their clinic significance. Methods Cerebrospinal fluid and plasma samples were collected from 20 patients with tuberculous meningitis (tuberculous meningitis group) and 20 patients suffered from primary headache (control group). The total RNAs were extracted.The levels of miR-125b, miR-29a and miR-155-5p were determined by real-time quantitative PCR (q-PCR). Results Levels of miR-29a and miR-125b in both CSF and plasma of patients in tuberculous meningitis group were significantly higher than those in pa⁃tients from control group with statistical significance (P<0.01). Mean time, the level of miR-155-5p in plasma but not in CSF of patients in tuberculous meningitis group was higher than those in control group with statistical significance ( P<0.01). Conclusion Expression of miR-125b, miR-29a and miR-155-5p may participate in regulating the occurrence and development of tuberculous meningitis. And miR-125b and miR-29a may be used as potential biomarkers for diagnosing tu⁃berculous meningitis.%目的:探讨结核性脑膜炎患者脑脊液及血浆中microRNA (miR)-125b、miR-29a和miR-155-5p的表达水平及其临床意义。方法收集20例结核性脑膜炎患者(结脑组)和20例原发性头痛患者(对照组)的脑脊液和血浆,分别提取总RNA,使用实时定量PCR方法分别检测脑脊液和血浆中miR-125b、miR-29a及miR-155-5p的表达水平。结果与对照组相比,结脑组脑脊液、血浆中miR-29a、miR-125b表达水平明显上调,结脑组血浆中miR-155-5p表达水平明显上调,差异均有统计学意义(均P<0.01),而2组脑脊液中miR-155-5p表达水平比较差异无统计学意义。结论 miR-125b、miR-29a和miR-155-5p可能参与了调节结核性脑膜炎的发生、发展过程,miR-125b和miR-29a有可能

  10. MiR-578 and miR-573 as potential players in BRCA-related breast cancer angiogenesis.

    Science.gov (United States)

    Danza, Katia; De Summa, Simona; Pinto, Rosamaria; Pilato, Brunella; Palumbo, Orazio; Merla, Giuseppe; Simone, Gianni; Tommasi, Stefania

    2015-01-01

    The involvement of microRNA (miRNAs), a new class of small RNA molecules, in governing angiogenesis has been well described. Our aim was to investigate miRNA-mediated regulation of angiogenesis in a series of familial breast cancers stratified by BRCA1/2 mutational status in BRCA carriers and BRCA non-carriers (BRCAX). Affymetrix GeneChip miRNA Arrays were used to perform miRNA expression analysis on 43 formalin-fixed paraffin-embedded (FFPE) tumour tissue familial breast cancers (22 BRCA 1/2-related and 21 BRCAX). Pathway enrichment analysis was carried out with the DIANA miRPath v2.0 web-based computational tool, and the miRWalk database was used to identify target genes of deregulated miRNAs. An independent set of 8 BRCA 1/2-related and 11 BRCAX breast tumors was used for validation by Real-Time PCR. In vitro analysis on HEK293, MCF-7 and SUM149PT cells were performed to best-clarify miR-573 and miR-578 role. A set of 16 miRNAs differentially expressed between BRCA 1/2-related and BRCAX breast tumors emerged from the profile analysis. Among these, miR-578 and miR-573 were found to be down-regulated in BRCA 1/2-related breast cancer and associated to the Focal adhesion, Vascular Endothelial Growth Factor (VEGF) and Hypoxia Inducible Factor-1 (HIF-1) signaling pathways. Our data highlight the role of miR-578 and miR-573 in controlling BRCA 1/2-related angiogenesis by targeting key regulators of Focal adhesion, VEGF and HIF-1 signaling pathways.

  11. A Biogenesis Step Upstream of Microprocessor Controls miR-17∼92 Expression.

    Science.gov (United States)

    Du, Peng; Wang, Longfei; Sliz, Piotr; Gregory, Richard I

    2015-08-13

    The precise control of miR-17∼92 microRNA (miRNA) is essential for normal development, and overexpression of certain miRNAs from this cluster is oncogenic. Here, we find that the relative expression of the six miRNAs processed from the primary (pri-miR-17∼92) transcript is dynamically regulated during embryonic stem cell (ESC) differentiation. Pri-miR-17∼92 is processed to a biogenesis intermediate, termed "progenitor-miRNA" (pro-miRNA). Pro-miRNA is an efficient substrate for Microprocessor and is required to selectively license production of pre-miR-17, pre-miR-18a, pre-miR-19a, pre-miR-20a, and pre-miR-19b from this cluster. Two complementary cis-regulatory repression domains within pri-miR-17∼92 are required for the blockade of miRNA processing through the formation of an autoinhibitory RNA conformation. The endonuclease CPSF3 (CPSF73) and the spliceosome-associated ISY1 are responsible for pro-miRNA biogenesis and expression of all miRNAs within the cluster except miR-92. Thus, developmentally regulated pro-miRNA processing is a key step controlling miRNA expression and explains the posttranscriptional control of miR-17∼92 expression in development.

  12. miRFam: an effective automatic miRNA classification method based on n-grams and a multiclass SVM

    Directory of Open Access Journals (Sweden)

    Zhou Shuigeng

    2011-05-01

    Full Text Available Abstract Background MicroRNAs (miRNAs are ~22 nt long integral elements responsible for post-transcriptional control of gene expressions. After the identification of thousands of miRNAs, the challenge is now to explore their specific biological functions. To this end, it will be greatly helpful to construct a reasonable organization of these miRNAs according to their homologous relationships. Given an established miRNA family system (e.g. the miRBase family organization, this paper addresses the problem of automatically and accurately classifying newly found miRNAs to their corresponding families by supervised learning techniques. Concretely, we propose an effective method, miRFam, which uses only primary information of pre-miRNAs or mature miRNAs and a multiclass SVM, to automatically classify miRNA genes. Results An existing miRNA family system prepared by miRBase was downloaded online. We first employed n-grams to extract features from known precursor sequences, and then trained a multiclass SVM classifier to classify new miRNAs (i.e. their families are unknown. Comparing with miRBase's sequence alignment and manual modification, our study shows that the application of machine learning techniques to miRNA family classification is a general and more effective approach. When the testing dataset contains more than 300 families (each of which holds no less than 5 members, the classification accuracy is around 98%. Even with the entire miRBase15 (1056 families and more than 650 of them hold less than 5 samples, the accuracy surprisingly reaches 90%. Conclusions Based on experimental results, we argue that miRFam is suitable for application as an automated method of family classification, and it is an important supplementary tool to the existing alignment-based small non-coding RNA (sncRNA classification methods, since it only requires primary sequence information. Availability The source code of miRFam, written in C++, is freely and publicly

  13. Expression and clinical value analysis of plasma miR-205, miR-212 and miR-429 in ovarian cancer%卵巢癌患者血浆miR-205、miR-212及miR-429的水平分析及意义∗

    Institute of Scientific and Technical Information of China (English)

    穆鹏; 李联崑; 贾海清; 王晓彬

    2015-01-01

    Objective To explore the levels of plasma miR⁃205, miR⁃212 and miR⁃429 in ovarian cancer and analyze the re⁃lationship between the above indicators and clinical pathology parameters of ovarian cancer. Methods Plasma samples from 71 ovarian cancer patients before treatment were collected. The real⁃time quantitative RT⁃PCR ( qRT⁃PCR) was used to detect the levels of miR⁃205, miR⁃212 and miR⁃429, and the clinical pathology parameters of ovarian cancer ( age, clinical stage, degree of differentiation, histological type and lymph node metastasis) were collected. The clinical pathology parameters of patients with different levels of miR⁃205, miR⁃212 and miR⁃429 were compared. The receiver operating characteristic curve ( ROC) was employed to analyze the clinical value of plasma miR⁃205, miR⁃212 and miR⁃429 in the diagnosis of ovarian cancer. Meanwhile, the plasma samples from 68 healthy volunteers (healthy control group) and 66 patients with benign ovarian tumor (benign group) were collected as control. Results There were higher levels of miR⁃205 and but lower miR⁃212 and miR⁃429 in ovarian cancer group versus other two groups with signifi⁃cant difference ( P all<0�05) . In ovarian cancer, the level of miR⁃205 was related with age, clinical stage, degree of differentiation and lymph node metastasis, and miR⁃212 was related with clinical stage and degree of differentiation, and miR⁃429 was related with clinical stage and lymph node metastasis ( P all<0�05) . The plasma level of miR⁃205 was negatively correlated with miR⁃212 and miR⁃429 ( r=-0�572、-0�325) , and miR⁃212 was positively correlated with miR⁃429 with statistically significance ( r=0�473,P<0�05) . The AUC, sensitivity and specificity of plasma miR⁃205, miR⁃212 and miR⁃429 in the diagnosis of ovarian cancer were 0�915, 92�1%and 86�2%, 0�944,87�3% and 91�0%, and 0�905, 88�5% and 83�6%. Conclusion There was higher

  14. Analytical Study of Hexapod miRNAs using Phylogenetic Methods

    CERN Document Server

    Mishra, A K

    2012-01-01

    MicroRNAs (miRNAs) are a class of non-coding RNAs that regulate gene expression. Identification of total number of miRNAs even in completely sequenced organisms is still an open problem. However, researchers have been using techniques that can predict limited number of miRNA in an organism. In this paper, we have used homology based approach for comparative analysis of miRNA of hexapoda group .We have used Apis mellifera, Bombyx mori, Anopholes gambiae and Drosophila melanogaster miRNA datasets from miRBase repository. We have done pair wise as well as multiple alignments for the available miRNAs in the repository to identify and analyse conserved regions among related species. Unfortunately, to the best of our knowledge, miRNA related literature does not provide in depth analysis of hexapods. We have made an attempt to derive the commonality among the miRNAs and to identify the conserved regions which are still not available in miRNA repositories. The results are good approximation with a small number of mis...

  15. Comparative analysis of known miRNAs across platyhelminths.

    Science.gov (United States)

    Jin, Xiaoliang; Lu, Lixia; Su, Hailong; Lou, Zhongzi; Wang, Fang; Zheng, Yadong; Xu, Guo-Tong

    2013-08-01

    MicroRNAs (miRNAs) are a subtype of small regulatory RNAs that are involved in numerous biological processes through small RNA-induced silencing networks. In an attempt to explore the phylogeny of miRNAs across five platyhelminths, we integrated annotated miRNAs and their full genomes. We identified conserved miRNA clusters and, in particular, miR-71/2 was conserved from planarian to parasitic flatworms and was expanded in free-living Schmidtea mediterranea. Analysis of 22 miRNA loci provided compelling evidence that most known miRNAs are conserved across platyhelminths. Meanwhile, we also observed alterations of known protein-coding genes flanking miRNA(s), such as transcriptional direction conversion and locus relocation, in around ~ 41% of 22 known miRNA loci. Compared with Echinococcus multilocularis, the majority of these events occurred in evolution-distant Hymenolepis microstoma, Schistosoma japonicum or/and S. mediterranea. These results imply rearrangement events occurred near the known miRNA loci.

  16. Characterization of miRNomes in Acute and Chronic Myeloid

    Directory of Open Access Journals (Sweden)

    Qian Xiong

    2014-04-01

    Full Text Available Myeloid leukemias are highly diverse diseases and have been shown to be associated with microRNA (miRNA expression aberrations. The present study involved an in-depth miRNome analysis of two human acute myeloid leukemia (AML cell lines, HL-60 and THP-1, and one human chronic myeloid leukemia (CML cell line, K562, via massively parallel signature sequencing. mRNA expression profiles of these cell lines that were established previously in our lab facilitated an integrative analysis of miRNA and mRNA expression patterns. miRNA expression profiling followed by differential expression analysis and target prediction suggested numerous miRNA signatures in AML and CML cell lines. Some miRNAs may act as either tumor suppressors or oncomiRs in AML and CML by targeting key genes in AML and CML pathways. Expression patterns of cell type-specific miRNAs could partially reflect the characteristics of K562, HL-60 and THP-1 cell lines, such as actin filament-based processes, responsiveness to stimulus and phagocytic activity. miRNAs may also regulate myeloid differentiation, since they usually suppress differentiation regulators. Our study provides a resource to further investigate the employment of miRNAs in human leukemia subtyping, leukemogenesis and myeloid development. In addition, the distinctive miRNA signatures may be potential candidates for the clinical diagnosis, prognosis and treatment of myeloid leukemias.

  17. miR-148 regulates Mitf in melanoma cells.

    Directory of Open Access Journals (Sweden)

    Benedikta S Haflidadóttir

    Full Text Available The Microphthalmia associated transcription factor (Mitf is an important regulator in melanocyte development and has been shown to be involved in melanoma progression. The current model for the role of Mitf in melanoma assumes that the total activity of the protein is tightly regulated in order to secure cell proliferation. Previous research has shown that regulation of Mitf is complex and involves regulation of expression, splicing, protein stability and post-translational modifications. Here we show that microRNAs (miRNAs are also involved in regulating Mitf in melanoma cells. Sequence analysis revealed conserved binding sites for several miRNAs in the Mitf 3'UTR sequence. Furthermore, miR-148 was shown to affect Mitf mRNA expression in melanoma cells through a conserved binding site in the 3'UTR sequence of mouse and human Mitf. In addition we confirm the previously reported effects of miR-137 on Mitf. Other miRNAs, miR-27a, miR-32 and miR-124 which all have conserved binding sites in the Mitf 3'UTR sequence did not have effects on Mitf. Our data show that miR-148 and miR-137 present an additional level of regulating Mitf expression in melanocytes and melanoma cells. Loss of this regulation, either by mutations or by shortening of the 3'UTR sequence, is therefore a likely factor in melanoma formation and/or progression.

  18. miR-375 and miR-30d in the Effect of Chromium-Containing Chinese Medicine Moderating Glucose Metabolism

    Directory of Open Access Journals (Sweden)

    Qian Zhang

    2014-01-01

    Full Text Available In China, TianMai Xiaoke tablet (TM is used to treat type 2 diabetes. However, the exact mechanism of TM is not clear. This study is to investigate the effect of TM on glucose metabolism in diabetic rats and to identify whether TM takes a direct action through microRNAs on islet. Rats were divided into control group, diabetic group, low dose of TM group (TML, and high dose of TM group (TMH. Pancreas samples were analyzed using microRNA array and Q-PCR. Eight-week treatment with TM significantly decreased fasting blood glucose. The blood glucose was significantly reduced in TM-treated groups before and after oral glucose administration. Fasting insulin and HOMA-IR were suppressed in TM-treated groups. miR-448, let-7b, miR-540, miR-296, miR-880, miR-200a, miR-500, miR-10b, miR-336, miR-30d, miR-208, let-7e, miR-142-5p, miR-874, miR-375, miR-879, miR-501, and miR-188 were upregulated, while miR-301b, miR-134, and miR-652 were downregulated in TMH group. Through target gene analysis and real-time PCR verification, we found that these miRNAs, especially miR-375 and miR-30d, can stimulate insulin secretion in islet. Our data suggest that TM can improve blood glucose in diabetic rats which involved increasing the expression of miR-375 and miR-30d to activate insulin synthesis in islet.

  19. Heterogeneity of miRNA expression in localized prostate cancer with clinicopathological correlations

    DEFF Research Database (Denmark)

    Zedan, Ahmed Hussein; Blavnsfeldt, Søren Garm; Hansen, Torben Frøstrup

    2017-01-01

    curatively intended surgery for localized PCa, were investigated with a panel of 6 miRNAs (miRNA-21, miRNA-34a, miRNA-125b, miRNA-126, miRNA-143, and miRNA-145) using tissue micro-array (TMA) and in situ hybridization (ISH). Inter- and intra-patient variation was assessed using intra-class correlation (ICC...

  20. Historic Context and Building Assessments for the Lawrence Livermore National Laboratory Built Environment

    Energy Technology Data Exchange (ETDEWEB)

    Ullrich, R. A. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Sullivan, M. A. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2007-09-14

    This document was prepared to support u.s. Department of Energy / National Nuclear Security Agency (DOE/NNSA) compliance with Sections 106 and 110 of the National Historic Preservation Act (NHPA). Lawrence Livermore National Laboratory (LLNL) is a DOE/NNSA laboratory and is engaged in determining the historic status of its properties at both its main site in Livermore, California, and Site 300, its test site located eleven miles from the main site. LLNL contracted with the authors via Sandia National Laboratories (SNL) to prepare a historic context statement for properties at both sites and to provide assessments of those properties of potential historic interest. The report contains an extensive historic context statement and the assessments of individual properties and groups of properties determined, via criteria established in the context statement, to be of potential interest. The historic context statement addresses the four contexts within which LLNL falls: Local History, World War II History (WWII), Cold War History, and Post-Cold War History. Appropriate historic preservation themes relevant to LLNL's history are delineated within each context. In addition, thresholds are identified for historic significance within each of the contexts based on the explication and understanding of the Secretary of the Interior's Guidelines for determining eligibility for the National Register of Historic Places. The report identifies specific research areas and events in LLNL's history that are of interest and the portions of the built environment in which they occurred. Based on that discussion, properties of potential interest are identified and assessments of them are provided. Twenty individual buildings and three areas of potential historic interest were assessed. The final recommendation is that, of these, LLNL has five individual historic buildings, two sets of historic objects, and two historic districts eligible for the National Register. All are

  1. Impact of Recent Constraints on Intellectual Freedom on Science and Technology at Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Wadsworth, J

    2000-11-12

    The Lawrence Livermore National Laboratory (LLNL) was created in 1952 to meet the nation's need for an expanded nuclear weapons research and development (R&D) capability. LLNL quickly grew to become a full-fledged nuclear weapons design laboratory with a broad range of technical capabilities similar to those of our sister laboratory--Los Alamos--with which we shared mission responsibilities. By its very nature, nuclear weapons R&D requires some of the most advanced science and technology (S&T). Accordingly, there is an obvious need for careful attention to ensure that appropriate security measures exist to deal with the sensitive aspects of nuclear weapons development. The trade-off between advancing S&T at the Laboratory and the need for security is a complex issue that has always been with us, As Edward Teller noted in a recent commentary in a May, 1999 editorial in the New York Times: ''The reaction of President Harry Truman to the leaking of information is well known. He imposed no additional measures for security. Instead, we have clear knowledge that the disclosures by (Klaus) Fuchs caused Truman to call for accelerated work on all aspects of nuclear weapons. The right prescription for safety is not reaction to dangers that are arising, but rather action leading to more knowledge and, one hopes, toward positive interaction between nations.'' To explore the issue of intellectual freedom at a national security laboratory such as LLNL, one must understand the type of activities we pursue and how our research portfolio has evolved since the Laboratory was established. Our mission affects the workforce skills, capabilities, and security measures that the Laboratory requires. The national security needs of the US have evolved, along with the S&T community in which the Laboratory resides and to which it contributes. These factors give rise to a greater need for the Laboratory to interact with universities, industry, and other national

  2. Year-class formation of upper St. Lawrence River northern pike

    Science.gov (United States)

    Smith, B.M.; Farrell, J.M.; Underwood, H.B.; Smith, S.J.

    2007-01-01

    Variables associated with year-class formation in upper St. Lawrence River northern pike Esox lucius were examined to explore population trends. A partial least-squares (PLS) regression model (PLS 1) was used to relate a year-class strength index (YCSI; 1974-1997) to explanatory variables associated with spawning and nursery areas (seasonal water level and temperature and their variability, number of ice days, and last day of ice presence). A second model (PLS 2) incorporated four additional ecological variables: potential predators (abundance of double-crested cormorants Phalacrocorax auritus and yellow perch Perca flavescens), female northern pike biomass (as a measure of stock-recruitment effects), and total phosphorus (productivity). Trends in adult northern pike catch revealed a decline (1981-2005), and year-class strength was positively related to catch per unit effort (CPUE; R2 = 0.58). The YCSI exceeded the 23-year mean in only 2 of the last 10 years. Cyclic patterns in the YCSI time series (along with strong year-classes every 4-6 years) were apparent, as was a dampening effect of amplitude beginning around 1990. The PLS 1 model explained over 50% of variation in both explanatory variables and the dependent variable, YCSI first-order moving-average residuals. Variables retained (N = 10; Wold's statistic ??? 0.8) included negative YCSI associations with high summer water levels, high variability in spring and fall water levels, and variability in fall water temperature. The YCSI exhibited positive associations with high spring, summer, and fall water temperature, variability in spring temperature, and high winter and spring water level. The PLS 2 model led to positive YCSI associations with phosphorus and yellow perch CPUE and a negative correlation with double-crested cormorant abundance. Environmental variables (water level and temperature) are hypothesized to regulate northern pike YCSI cycles, and dampening in YCSI magnitude may be related to a

  3. Heavy ion facilities and heavy ion research at Lawrence Berkeley Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1973-10-01

    Lawrence Berkeley Laboratory has been heavily involved since 1956 in the construction and adaptation of particle accelerators for the acceleration of heavy ions. At the present time it has the most extensive group of accelerators with heavy-ion capability in the United States: The SuperHILAC, the 88-Inch Cyclotron, and the Bevatron/Bevalac. An extensive heavy-ion program in nuclear and particle physics, in nuclear chemistry, and in the study of biological effects of heavy-ion irradiations has been supported in the past; and the Laboratory has a strong interest in expanding both its capabilities for heavy-ion acceleration and its participation in heavy-ion science. The first heavy-ion accelerator at LBL was the HILAC, which began operation in 1957. A vigorous program of research with ion beams of masses 4 through 40 began at that time and continued until the machine was shut down for modifications in February 1971. At that time, a grant of $3 M had been received from the AEC for a total reconstruction of the HILAC, to turn it into an upgraded accelerator, the SuperHILAC. This new machine is designed for the acceleration of all ions through uranium to an energy of 8.5 MeV/u. The SuperHILAC is equipped with two injectors. The lower energy injector, a 750-kV Cockcroft-Walton machine, was put into service in late 1972 for acceleration of ions up through {sup 40}Ar. By spring of 1973, operation of the SuperHILAC with this injector exceeded the performance of the original HILAC. The second injector, a 2.5-MV Dynamitron, was originally designed for the Omnitron project and built with $1 M of Omnitron R and D funds. Commissioning of this injector began in 1973 and proceeded to the point where nanoampere beams of krypton were available for a series of research studies in May and June. The first publishable new results with beams heavier than {sup 40}Ar were obtained at that time. Debugging and injector improvement projects will continue in FY 74.

  4. Final Progress Report for the NASA Inductrack Model Rocket Launcher at the Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Tung, L S; Post, R F; Martinez-Frias, J

    2001-06-27

    The Inductrack magnetic levitation system, developed at the Lawrence Livermore National Laboratory, was studied for its possible use for launching rockets. Under NASA sponsorship, a small model system was constructed at the Laboratory to pursue key technical aspects of this proposed application. The Inductrack is a passive magnetic levitation system employing special arrays of high-field permanent magnets (Halbach arrays) on the levitating cradle, moving above a ''track'' consisting of a close-packed array of shorted coils with which are interleaved with special drive coils. Halbach arrays produce a strong spatially periodic magnetic field on the front surface of the arrays, while canceling the field on their back surface. Relative motion between the Halbach arrays and the track coils induces currents in those coils. These currents levitate the cradle by interacting with the horizontal component of the magnetic field. Pulsed currents in the drive coils, synchronized with the motion of the carrier, interact with the vertical component of the magnetic field to provide acceleration forces. Motional stability, including resistance to both vertical and lateral aerodynamic forces, is provided by having Halbach arrays that interact with both the upper and the lower sides of the track coils. At present, a 7.8 meter track composed of drive and levitation coils has been built and the electronic drive circuitry performs as designed. A 9 kg cradle that carries the Halbach array of permanent magnets has been built. A mechanical launcher is nearly complete which will provide an initial cradle velocity of 9 m/s into the electronic drive section. We have found that the drag forces from the levitation coils were higher than in our original design. However, measurements of drag force at velocities less than 1 m/s are exactly as predicted by theory. Provided here are recommended design changes to improve the track's performance so that a final velocity of 40

  5. Lawrence Livermore National Laboratory Workshop Characterization of Pathogenicity, Virulence and Host-Pathogen Interactions

    Energy Technology Data Exchange (ETDEWEB)

    Krishnan, A

    2006-08-30

    The threats of bio-terrorism and newly emerging infectious diseases pose serious challenges to the national security infrastructure. Rapid detection and diagnosis of infectious disease in human populations, as well as characterizing pathogen biology, are critical for reducing the morbidity and mortality associated with such threats. One of the key challenges in managing an infectious disease outbreak, whether through natural causes or acts of overt terrorism, is detection early enough to initiate effective countermeasures. Much recent attention has been directed towards the utility of biomarkers or molecular signatures that result from the interaction of the pathogen with the host for improving our ability to diagnose and mitigate the impact of a developing infection during the time window when effective countermeasures can be instituted. Host responses may provide early signals in blood even from localized infections. Multiple innate and adaptive immune molecules, in combination with other biochemical markers, may provide disease-specific information and new targets for countermeasures. The presence of pathogen specific markers and an understanding of the molecular capabilities and adaptations of the pathogen when it interacts with its host may likewise assist in early detection and provide opportunities for targeting countermeasures. An important question that needs to be addressed is whether these molecular-based approaches will prove useful for early diagnosis, complement current methods of direct agent detection, and aid development and use of countermeasures. Lawrence Livermore National Laboratory (LLNL) will host a workshop to explore the utility of host- and pathogen-based molecular diagnostics, prioritize key research issues, and determine the critical steps needed to transition host-pathogen research to tools that can be applied towards a more effective national bio-defense strategy. The workshop will bring together leading researchers/scientists in the

  6. Evaluation of historical beryllium abundance in soils, airborne particulates and facilities at Lawrence Livermore National Laboratory.

    Science.gov (United States)

    Sutton, Mark; Bibby, Richard K; Eppich, Gary R; Lee, Steven; Lindvall, Rachel E; Wilson, Kent; Esser, Bradley K

    2012-10-15

    Beryllium has been historically machined, handled and stored in facilities at Lawrence Livermore National Laboratory (LLNL) since the 1950s. Additionally, outdoor testing of beryllium-containing components has been performed at LLNL's Site 300 facility. Beryllium levels in local soils and atmospheric particulates have been measured over three decades and are comparable to those found elsewhere in the natural environment. While localized areas of beryllium contamination have been identified, laboratory operations do not appear to have increased the concentration of beryllium in local air or water. Variation in airborne beryllium correlates to local weather patterns, PM10 levels, normal sources (such as resuspension of soil and emissions from coal power stations) but not to LLNL activities. Regional and national atmospheric beryllium levels have decreased since the implementation of the EPA's 1990 Clean-Air-Act. Multi-element analysis of local soil and air samples allowed for the determination of comparative ratios for beryllium with over 50 other metals to distinguish between natural beryllium and process-induced contamination. Ten comparative elemental markers (Al, Cs, Eu, Gd, La, Nd, Pr, Sm, Th and Tl) that were selected to ensure background variations in other metals did not collectively interfere with the determination of beryllium sources in work-place samples at LLNL. Multi-element analysis and comparative evaluation are recommended for all workplace and environmental samples suspected of beryllium contamination. The multi-element analyses of soils and surface dusts were helpful in differentiating between beryllium of environmental origin and beryllium from laboratory operations. Some surfaces can act as "sinks" for particulate matter, including carpet, which retains entrained insoluble material even after liquid based cleaning. At LLNL, most facility carpets had beryllium concentrations at or below the upper tolerance limit determined by sampling facilities

  7. The Roles of miR-26, miR-29, and miR-203 in the Silencing of the Epigenetic Machinery during Melanocyte Transformation.

    Science.gov (United States)

    Gasque Schoof, Cláudia Regina; Izzotti, Alberto; Jasiulionis, Miriam Galvonas; Vasques, Luciana Dos Reis

    2015-01-01

    The epigenetic marks located throughout the genome exhibit great variation between normal and transformed cancer cells. While normal cells contain hypomethylated CpG islands near gene promoters and hypermethylated repetitive DNA, the opposite pattern is observed in cancer cells. Recently, it has been reported that alteration in the microenvironment of melanocyte cells, such as substrate adhesion blockade, results in the selection of anoikis-resistant cells, which have tumorigenic characteristics. Melanoma cells obtained through this model show an altered epigenetic pattern, which represents one of the first events during the melanocytes malignant transformation. Because microRNAs are involved in controlling components of the epigenetic machinery, the aim of this work was to evaluate the potential association between the expression of miR-203, miR-26, and miR-29 family members and the genes Dnmt3a, Dnmt3b, Mecp2, and Ezh2 during cells transformation. Our results show that microRNAs and their validated or predicted targets are inversely expressed, indicating that these molecules are involved in epigenetic reprogramming. We also show that miR-203 downregulates Dnmt3b in mouse melanocyte cells. In addition, treatment with 5-aza-CdR promotes the expression of miR-26 and miR-29 in a nonmetastatic melanoma cell line. Considering the occurrence of CpG islands near the miR-26 and miR-29 promoters, these data suggest that they might be epigenetically regulated in cancer.

  8. 慢性心力衰竭患者血浆miRNA表达谱分析%Profiling of plasma miRNA in chronic heart failure patients

    Institute of Scientific and Technical Information of China (English)

    李璐; 陈群蓉; 纪玲

    2016-01-01

    目的:对慢性心力衰竭(CHF)患者的血浆miRNA进行二代测序,探讨CHF患者血浆中miRNA的表达差异。方法收集2013年7月至2015年6月间在北京大学深圳医院治疗的40例CHF患者和10例健康对照个体,应用第二代高通量测序技术对其血浆miRNA进行测序,寻找差异表达的miRNA。另收集同期CHF患者和健康对照个体各96例,用逆转录-实时荧光定量聚合酶链反应对差异表达的miRNA进行验证。结果与健康对照者相比,高通量测序显示在CHF患者的血浆中miR-184、miR-651-5p、miR-130b-5p、miR-203a-3p、miR-548e-3p和miR-106a-5p的表达水平存在显著上调,miR-31-5p和miR-5091的表达水平存在下调,逆转录-实时荧光定量聚合酶链反应检测血浆miRNA的表达差异与高通量测序的结果相符。结论 CHF患者的血浆miRNA与健康个体的表达谱存在差异,差异表达的miR-184、miR-651-5p、miR-130b-5p、miR-203a-3p、miR-548e-3p、miR-106a-5p、miR-31-5p和miR-5091或许与CHF的发生有关。%Objective To search for differentially expressed plasma miRNAs between patients with chronic heart failure (CHF) and healthy controls by next-generation small RNA sequencing. Methods Forty CHF patients and 10 healthy volunteers were recruited to search for differentially expressed plasma miRNAs in next-generation small RNA sequencing study from Peking University Shenzhen Hospital between July 2013 and June 2015. miRNAs expres-sion were validated in another 96 patients and 96 controls by RT-PCR analysis during the same period. Results Com-paring to healthy group, miRNA sequencing showed that miR-184, miR-651-5p, miR-130b-5p, miR-203a-3p, miR-548e-3p, and miR-106a-5p were upregulated and miR-31-5p and miR-5091 were downregulated in CHF pa-tients. The levels of miRNA measured by RT-PCR were consistent with the results obtained from miRNA sequencing technology. Conclusion A differentially expressed miRNA profiling was seen between

  9. miR-181a and miR-630 regulate cisplatin-induced cancer cell death.

    Science.gov (United States)

    Galluzzi, Lorenzo; Morselli, Eugenia; Vitale, Ilio; Kepp, Oliver; Senovilla, Laura; Criollo, Alfredo; Servant, Nicolas; Paccard, Caroline; Hupé, Philippe; Robert, Thomas; Ripoche, Hugues; Lazar, Vladimir; Harel-Bellan, Annick; Dessen, Philippe; Barillot, Emmanuel; Kroemer, Guido

    2010-03-01

    MicroRNAs (miRNA) are noncoding RNAs that regulate multiple cellular processes, including proliferation and apoptosis. We used microarray technology to identify miRNAs that were upregulated by non-small cell lung cancer (NSCLC) A549 cells in response to cisplatin (CDDP). The corresponding synthetic miRNA precursors (pre-miRNAs) per se were not lethal when transfected into A549 cells yet affected cell death induction by CDDP, C2-ceramide, cadmium, etoposide, and mitoxantrone in an inducer-specific fashion. Whereas synthetic miRNA inhibitors (anti-miRNAs) targeting miR-181a and miR-630 failed to modulate the response of A549 to CDDP, pre-miR-181a and pre-miR-630 enhanced and reduced CDDP-triggered cell death, respectively. Pre-miR-181a and pre-miR-630 consistently modulated mitochondrial/postmitochondrial steps of the intrinsic pathway of apoptosis, including Bax oligomerization, mitochondrial transmembrane potential dissipation, and the proteolytic maturation of caspase-9 and caspase-3. In addition, pre-miR-630 blocked early manifestations of the DNA damage response, including the phosphorylation of the ataxia-telangiectasia mutated (ATM) kinase and of two ATM substrates, histone H2AX and p53. Pharmacologic and genetic inhibition of p53 corroborated the hypothesis that pre-miR-630 (but not pre-miR-181a) blocks the upstream signaling pathways that are ignited by DNA damage and converge on p53 activation. Pre-miR-630 arrested A549 cells in the G0-G1 phase of the cell cycle, correlating with increased levels of the cell cycle inhibitor p27(Kip1) as well as with reduced proliferation rates and resulting in greatly diminished sensitivity of A549 cells to the late S-G2-M cell cycle arrest mediated by CDDP. Altogether, these results identify miR-181a and miR-630 as novel modulators of the CDDP response in NSCLC.

  10. MiPS (Mi Prostate Score Urine test) — EDRN Public Portal

    Science.gov (United States)

    The MiPS assay is a multiplex analysis of T2-ERG gene fusion, PCA3, and serum PSA (KLK3). It is commercially available through the University of Michigan MLabs. The MiPS assay tests for the presence of two prostate cancer biomarkers: a piece of RNA made from the PCA3 gene, found to be overactive in 95 percent of all prostate cancers, and another RNA marker that is found only when TMPRSS2 and ERG abnormally fuse. TMPRSS2:ERG, or T2-ERG, is a strong indicator of prostate cancer.

  11. Expression and clinical significance of miR-29c, miR-133b and miR-1 in ovarian cancer tissue%卵巢癌组织中miR-29c、miR-133b和miR-1的水平分析及临床意义

    Institute of Scientific and Technical Information of China (English)

    穆鹏; 张菁如

    2015-01-01

    Objective To explore the expressions of miR⁃29c, miR⁃133b and miR⁃1 in ovarian cancer tissue and analyze the relationship between the above indicators and clinical pathology parameters of ovarian cancer. Methods Cancer tissue from 65 patients with ovarian cancer were collected as cancer group. The real⁃time quantitative PCR ( qRT⁃PCR) was used to detect the expressions of miR⁃29c, miR⁃133b and miR⁃1 and the clinical pathology parameters of ovarian cancer ( age, clinical stage, degree of differentiation, histological type and lymph node metastasis) were collected. Meanwhile, 32 cases of normal ovarian epithelial tissues ( normal group) and 39 cases of benign ovarian cyst tissue ( benign group) were taken as control. The average level of three indices of normal group were chosen as boundary value, and then the patients were categorized into high⁃level group (>boundary value) and low⁃level group (≤boundary value) . The clinical pathology parameters of patients with different levels of miR⁃29c, miR⁃133b and miR⁃1 were com⁃pared. The relationships among miR⁃29c, miR⁃133b and miR⁃1 were investigated in cancer group. Results There were higher levels of miR⁃29c and but lower miR⁃133b and miR⁃1 in ovarian cancer group versus other two groups with significant difference ( P<0�05) . In ovarian cancer, the levels of three miRNAs were related with degree of differentiation, and miR⁃29c was related with clinical stage and lymph node metastasis, and miR⁃133b was related with lymph node metastasis (P<0�05). The level of miR⁃29c was negatively correlated with miR⁃133b and miR⁃1 ( r=-0�541, r=-0�361) , and miR⁃133b was positively correlated with miR⁃1 with statistically significance ( P<0�05) . Conclusion There were higher expression of miR⁃29c but lower expression of miR⁃133b and miR⁃1. The lev⁃els of the above miRNAs were related with clinical pathology parameters in ovarian cancer tissue, showing a certain

  12. Expression of miR-155 and miR-126 in situ in cutaneous T-cell lymphoma

    DEFF Research Database (Denmark)

    Kopp, Katharina L; Ralfkiaer, Ulrik; Nielsen, Boye S;

    2013-01-01

    Recently, miR-155 has been implicated in cutaneous T-cell lymphoma (CTCL). Thus, elevated levels of miR-155 were observed in skin lesions from CTCL patients as judged from qPCR and micro-array analysis and aberrant, high miR-155 expression was associated with severe disease. Moreover, miR-155...... promoted proliferation of malignant T cells in vitro. Little is, however, known about which cell types express miR-155 in vivo in CTCL skin lesions. Here, we study miR-155 expression using in situ hybridization (ISH) with a miR-155 probe, a negative control (scrambled), and a miR-126 probe as a positive...... control in nine patients with mycosis fungoides, the most frequent subtype of CTCL. We provide evidence that both malignant and non-malignant T cells stain weakly to moderately positive with the miR-155 probe, but generally negative with the miR-126 and negative control probes. Reversely, endothelial...

  13. miR-29 and human diseases%微RNA miR-29与人类疾病

    Institute of Scientific and Technical Information of China (English)

    黄昌发; 刘小珊

    2010-01-01

    人类微RNA miR-29包括miR-29a、miR-29b1、miR-29b2和miR-29c.miR-29在胚胎期组织不表达或低表达,而在生物体成熟组织细胞中广泛表达.实验证实的miR-29作用的靶点包括DNMT3A/3B、YY1、Mcl-1、BACE1以及HIV-1病毒等.miR-29与人类疾病密切相关,miR-29在多种肿瘤细胞、心肌梗塞与散发性阿尔茨海默病等病变组织细胞中表达受抑.本文就miR-29基因结构,基因表达与调控、生物学功能以及与疾病发生相关性作一综述.

  14. Quantitative proteomic analysis of gene regulation by miR-34a and miR-34c.

    Directory of Open Access Journals (Sweden)

    Olivia A Ebner

    Full Text Available microRNAs (miRNAs repress target genes by destabilizing mRNAs and/or by inhibiting translation. The best known factor for target recognition is the so called seed--a short continuous region of Watson-Crick base pairing between nucleotides 2-7 of the miRNA and complementary sequences in 3' untranslated regions of target mRNAs. The miR-34 family consists of three conserved members with important tumor suppressor functions linked to the p53 pathway. The family members share the same seed, raising the question if they also have the same targets. Here, we analyse the effect of miR-34a and miR-34c on protein synthesis by pSILAC. Despite significant overlap, we observe that the impact of both family members on protein synthesis differs. The ability to identify specific targets of a family member is complicated by the occurrence of * strand mediated repression. Transfection of miR-34 chimeras indicates that the 3'end of the miRNA might be responsible for differential regulation in case of targets without a perfect seed site. Pathway analysis of regulated proteins indicates overlapping functions related to cell cycle and the p53 pathway and preferential targeting of several anti-apoptotic proteins by miR-34a. We used luciferase assays to confirm that Vcl and Fkbp8, an important anti-apoptotic protein, are specifically repressed by miR-34a. In summary, we find that miR-34a and miR-34c down-regulate distinct subsets of targets which might mediate different cellular outcomes. Our data provides a rich resource of miR-34 targets that might be relevant for clinical trials that want to implement the miR-34 family in cancer therapy.

  15. MicroRNA Detection Using a Double Molecular Beacon Approach: Distinguishing Between miRNA and Pre-miRNA.

    Science.gov (United States)

    James, Amanda Marie; Baker, Meredith B; Bao, Gang; Searles, Charles D

    2017-01-01

    MicroRNAs (miRNAs) are small, noncoding RNAs that post-transcriptionally regulate gene expression and are recognized for their roles both as modulators of disease progression and as biomarkers of disease activity, including neurological diseases, cancer, and cardiovascular disease (CVD). Commonly, miRNA abundance is assessed using quantitative real-time PCR (qRT-PCR), however, qRT-PCR for miRNA can be labor intensive, time consuming, and may lack specificity for detection of mature versus precursor forms of miRNA. Here, we describe a novel double molecular beacon approach to miRNA assessment that can distinguish and quantify mature versus precursor forms of miRNA in a single assay, an essential feature for use of miRNAs as biomarkers for disease. Using this approach, we found that molecular beacons with DNA or combined locked nucleic acid (LNA)-DNA backbones can detect mature and precursor miRNAs (pre-miRNAs) of low (beacon assay was accurate in assessing miRNA abundance in a sample containing a mixed population of mature and precursor miRNAs. In contrast, qRT-PCR and the single molecular beacon assay overestimated miRNA abundance. Additionally, the double molecular beacon assay was less labor intensive than traditional qRT-PCR and had 10-25% increased specificity. Our data suggest that the double molecular beacon-based approach is more precise and specific than previous methods, and has the promise of being the standard for assessing miRNA levels in biological samples.

  16. miR-203 and miR-205 expression patterns identify subgroups of prognosis in cutaneous squamous cell carcinoma.

    Science.gov (United States)

    Cañueto, J; Cardeñoso-Álvarez, E; García-Hernández, J L; Galindo-Villardón, P; Vicente-Galindo, P; Vicente-Villardón, J L; Alonso-López, D; De Las Rivas, J; Valero, J; Moyano-Sáez, E; Fernández-López, E; Mao, J H; Castellanos-Martín, A; Román-Curto, C; Pérez-Losada, J

    2016-12-11

    Cutaneous squamous cell carcinoma is the second most widespread cancer in humans and its incidence is rising. These tumours can evolve as poor-prognosis diseases, and therefore it is important to identify new markers to better predict its clinical evolution. Here, we identified the expression pattern of miRNAs at different stages of skin cancer progression in a panel of murine skin cancer cell lines. We determined that miR-203 and miR-205 are differentially expressed in this panel, and evaluated their potential use as biomarkers of prognosis in human tumours. MiR-205 was expressed in tumours with pathological features recognized as indicators of poor prognosis such as desmoplasia, perineural invasion and infiltrative growth pattern. MiR-205 was mainly expressed in undifferentiated areas and in the invasion front, and was associated with both local recurrence and the development of general clinical events of poor evolution. MiR-205 expression was an independent variable selected to predict events of poor clinical evolution using the multinomial logistic regression model described in this study. In contrast, miR-203 was mainly expressed in tumours exhibiting the characteristics associated with a good prognosis, was mainly present in well-differentiated zones, and rarely expressed in the invasion front. Therefore, the expression and associations of miR-205 and miR-203 were mostly mutually exclusive. Finally, using a logistic biplot we identified three clusters of patients with differential prognosis based on miR-203 and miR-205 expression, and pathological tumour features. This work highlights the utility of miRNA-205 and miRNA-203 as prognostic markers in cutaneous squamous cell carcinoma. This article is protected by copyright. All rights reserved.

  17. Identification of targets of miRNA-221 and miRNA-222 in fulvestrant-resistant breast cancer

    Science.gov (United States)

    Liu, Pengfei; Sun, Manna; Jiang, Wenhua; Zhao, Jinkun; Liang, Chunyong; Zhang, Huilai

    2016-01-01

    The present study aimed to identify the differentially expressed genes (DEGs) regulated by microRNA (miRNA)-221 and miRNA-222 that are associated with the resistance of breast cancer to fulvestrant. The GSE19777 transcription profile was downloaded from the Gene Expression Omnibus database, and includes data from three samples of antisense miRNA-221-transfected fulvestrant-resistant MCF7-FR breast cancer cells, three samples of antisense miRNA-222-transfected fulvestrant-resistant MCF7-FR cells and three samples of control inhibitor (green fluorescent protein)-treated fulvestrant-resistant MCF7-FR cells. The linear models for microarray data package in R/Bioconductor was employed to screen for DEGs in the miRNA-transfected cells, and the pheatmap package in R was used to perform two-way clustering. Pathway enrichment was conducted using the Gene Set Enrichment Analysis tool. Furthermore, a miRNA-messenger (m) RNA regulatory network depicting interactions between miRNA-targeted upregulated DEGs was constructed and visualized using Cytoscape. In total, 492 and 404 DEGs were identified for the antisense miRNA-221-transfected MCF7-FR cells and the antisense miRNA-222-transfected MCF7-FR cells, respectively. Genes of the pentose phosphate pathway (PPP) were significantly enriched in the antisense miRNA-221-transfected MCF7-FR cells. In addition, components of the Wnt signaling pathway and cell adhesion molecules (CAMs) were significantly enriched in the antisense miRNA-222-transfected MCF7-FR cells. In the miRNA-mRNA regulatory network, miRNA-222 was demonstrated to target protocadherin 10 (PCDH10). The results of the present study suggested that the PPP and Wnt signaling pathways, as well as CAMs and PCDH10, may be associated with the resistance of breast cancer to fulvestrant. PMID:27895744

  18. 先天性心脏病相关性肺动脉高压患者血miR-18a、miR-27b、miR-130a、miR-204水平研究%The changes of plasma miR-18a, miR-27b, miR-130a, miR-204 in patients with pulmonary arterial hypertension due to congenital heart disease

    Institute of Scientific and Technical Information of China (English)

    吕宁; 戴海龙; 尹小龙

    2014-01-01

    目的 探索先天性心性脏病相关性肺动脉高压(PAH)患者血清中miR-18a、miR-27b、miR-130a和miR-204的表达水平,以及与PAH的相关性.方法 收集昆明医科大学附属延安医院心血管内科2012年4~12月被确诊为先天性心脏病的患者78例,其中肺动脉压正常组37例,合并轻、中度肺动脉高压组25例,重度肺动脉高压组16例;另设正常对照组20例.应用实时荧光定量PCR (Taqman探针法)检测miR-18a、miR-27b、miR-130a、miR-204在血液中的表达水平,分析先天性心脏病相关性肺动脉高压与miR-NA表达水平之间的关系.结果 先天性心脏病相关肺动脉高压患者血miR-18a、miR-27b、miR-130a表达水平显著上调,miR-204表达水平显著下调.随着肺动脉压力的不断增高,血miR-18a、miR-27b、miR-130a表达量明显升高,miR-204表达量显著下调.直线相关分析显示,先天性心脏病继发性肺动脉高压患者血miR-18a、miR-27b、miR-130a表达水平与肺动脉压力存在高度正相关关系(r=0.927,r=0.927,r=0.933,P<0.01),miR-204表达水平与肺动脉压力存在高度负相关关系(r=-0.773,P<0.01).结论 血miR-18a、miR-27b、miR-130a、miR-204水平可作为判断先天性心脏病合并肺动脉高压较好的临床血清学指标,预判价值较高.

  19. 脑胶质瘤患者血清miR-21、miR-221、miR-222和miR-10b的相对表达量及临床意义%The Relative Expression of Serum miR-21 ,miR-221 ,miR-222 and miR-10b in Patients with Glioma and Its Clinical Significance

    Institute of Scientific and Technical Information of China (English)

    朱建军; 李春生

    2015-01-01

    目的 探讨血清中miR-21、miR-221、miR-222和miR-10b对脑胶质瘤恶性程度的鉴别价值.方法 采用Real-timePCR技术检测34例低级别脑胶质瘤患者和50例高级别脑胶质瘤患者miR-21、miR-221、miR-222和miR-10b的相对表达量,使用受试者工作特征曲线(receiver operating characteristic curve,ROC)评价其诊断价值.结果 与低级别脑胶质瘤组相比,高级别脑胶质瘤组血清miR-10b、miR-21和miR-221的相对表达量均显著升高,且差异具有统计学意义,miR-222相对表达量的差异无统计学意义.ROC曲线评价血清miR-10b、miR-21和miR-221对于脑胶质瘤恶性程度的诊断价值,miR-10b的曲线下面积(area under curve,AUC)值最大,为0.722(0.694,0.751),对于低级别脑胶质瘤的诊断敏感性和特异性分别为79.41%和78.00%.使用二元Logistic回归分析评价血清miR-10b、miR-21和miR-221联合检测对于脑胶质瘤恶性程度的诊断价值,其曲线下面积为0.915(0.855,0.977),对于低级别脑胶质瘤的诊断敏感性和特异性分别为85.29%和88.00%.与miR-10b、miR-21和miR-221单独检测的诊断价值相比,其曲线下面积均有显著提高(P=0.026、P=0.012、P=0.008).结论 miR-10b、miR-21和miR-221联合诊断可以为临床脑胶质瘤患者的鉴别诊断提供一种潜在的辅助诊断方法.

  20. Goldin ja Katz meeste ning naiste palgalõhest / Claudia Goldin, Lawrence Katz ; toimetanud Steven D. Levitt, Stephen Dubner

    Index Scriptorium Estoniae

    Goldin, Claudia

    2010-01-01

    Harvardi ülikooli tööturu uurimisele spetsialiseerunud majandusteadlased ning meeste ja naiste palgalõhe teema spetsialistid Claudia Goldin ja Lawrence Katz vastavad küsimustele, mis puudutavad võrdse haridusega meeste ja naiste tegelikku palgaerinevust, palgalõhet meeste ja naiste vahel siis, kui mitte arvestada tööaega, naiste suutlikkust pidada palgaläbirääkimisi ning tegevusalasi, kuhu peaksid mehed ja naised pürgima või mida vältima