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Sample records for monodon tata box-binding

  1. Differential stability of TATA box binding proteins from archaea with different optimal growth temperatures

    Science.gov (United States)

    Kopitz, Annette; Soppa, Jörg; Krejtschi, Carsten; Hauser, Karin

    2009-09-01

    The TATA box binding protein (TBP) is involved in promoter recognition, the first step of transcription initiation. TBP is universally conserved and essential in archaea and eukaryotes. In archaea, TBPs have to be stable and to function in species that cover an extremely wide range of optimal growth temperatures (OGTs), from below 0 °C to more than 100 °C. Thus, the archaeal TBP family is ideally suited to study the evolutionary adaptation of proteins to an extremely wide range of temperatures. We characterized the thermostability of one mesophilic and one thermophilic TBP by infrared spectroscopy. Transition temperatures ( Tms) of thermal unfolding have been determined using TBPs from Methanosarcina mazei (OGT 37 °C) and from Methanothermobacter thermautotrophicus (OGT 65 °C). Furthermore, the influence of protein and salt concentration on thermostability has been characterized. Together with previous studies, our results reveal that the Tms of archaeal TBPs are closely correlated with the OGTs of the respective species. Noteworthy, this is also true for the TBP from M. mazei representing the first characterized TBP from a mesophilic archaeon. In contrast, the only characterized eukaryotic TBP of the mesophilic plant Arabidopsis thaliana has a Tm more than 40 °C above the OGT.

  2. Molecular Cloning of a cDNA Encoding for Taenia solium TATA-Box Binding Protein 1 (TsTBP1) and Study of Its Interactions with the TATA-Box of Actin 5 and Typical 2-Cys Peroxiredoxin Genes.

    Science.gov (United States)

    Rodríguez-Lima, Oscar; García-Gutierrez, Ponciano; Jiménez, Lucía; Zarain-Herzberg, Ángel; Lazzarini, Roberto; Landa, Abraham

    2015-01-01

    TATA-box binding protein (TBP) is an essential regulatory transcription factor for the TATA-box and TATA-box-less gene promoters. We report the cloning and characterization of a full-length cDNA that encodes a Taenia solium TATA-box binding protein 1 (TsTBP1). Deduced amino acid composition from its nucleotide sequence revealed that encodes a protein of 238 residues with a predicted molecular weight of 26.7 kDa, and a theoretical pI of 10.6. The NH2-terminal domain shows no conservation when compared with to pig and human TBP1s. However, it shows high conservation in size and amino acid identity with taeniids TBP1s. In contrast, the TsTBP1 COOH-terminal domain is highly conserved among organisms, and contains the amino acids involved in interactions with the TATA-box, as well as with TFIIA and TFIIB. In silico TsTBP1 modeling reveals that the COOH-terminal domain forms the classical saddle structure of the TBP family, with one α-helix at the end, not present in pig and human. Native TsTBP1 was detected in T. solium cysticerci´s nuclear extract by western blot using rabbit antibodies generated against two synthetic peptides located in the NH2 and COOH-terminal domains of TsTBP1. These antibodies, through immunofluorescence technique, identified the TBP1 in the nucleus of cells that form the bladder wall of cysticerci of Taenia crassiceps, an organism close related to T. solium. Electrophoretic mobility shift assays using nuclear extracts from T. solium cysticerci and antibodies against the NH2-terminal domain of TsTBP1 showed the interaction of native TsTBP1 with the TATA-box present in T. solium actin 5 (pAT5) and 2-Cys peroxiredoxin (Ts2-CysPrx) gene promoters; in contrast, when antibodies against the anti-COOH-terminal domain of TsTBP1 were used, they inhibited the binding of TsTBP1 to the TATA-box of the pAT5 promoter gene.

  3. Regulation of activity of the yeast TATA-binding protein through intra ...

    Indian Academy of Sciences (India)

    Unknown

    Abbreviations used: BMH, Bismaleimidohexane; TBP, TATA-binding protein; yTBP, yeast TBP. J. Biosci. | Vol. ... Therefore for full-length TBP, intra-molecular interactions can regulate its activity via a similar ..... simulations (Miaskeiwicz and Ornstein 1996). .... box binding protein (TBP): A molecular dynamics computa-.

  4. TATA-binding protein and the retinoblastoma gene product bind to overlapping epitopes on c-Myc and adenovirus E1A protein

    NARCIS (Netherlands)

    Hateboer, G.; Timmers, H.T.M.; Rustgi, A.K.; Billaud, Marc; Veer, L.J. Van 't; Bernards, R.A.

    1993-01-01

    Using a protein binding assay, we show that the amino-teminal 204 amino acids of the c-Myc protein interact di y with a key component of the basal p tdon factor TFID, the TATA box-binding protein (TBP). Essentialy the same region of the c-Myc protein alo binds the product of the retinoblatoma

  5. TAF4 nucleates a core subcomplex of TFIID and mediates activated transcription from a TATA-less promoter

    OpenAIRE

    Wright, Kevin J.; Marr, Michael T.; Tjian, Robert

    2006-01-01

    Activator-dependent recruitment of TFIID initiates formation of the transcriptional preinitiation complex. TFIID binds core promoter DNA elements and directs the assembly of other general transcription factors, leading to binding of RNA polymerase II and activation of RNA synthesis. How TATA box-binding protein (TBP) and the TBP-associated factors (TAFs) are assembled into a functional TFIID complex with promoter recognition and coactivator activities in vivo remains unknown. Here, we use RNA...

  6. Dynamics of TBP binding to the TATA box

    Science.gov (United States)

    Schluesche, Peter; Heiss, Gregor; Meisterernst, Michael; Lamb, Don C.

    2008-02-01

    Gene expression is highly controlled and regulated in living cells. One of the first steps in gene transcription is recognition of the promoter site by the TATA box Binding Protein (TBP). TBP recruits other transcriptions factors and eventually the RNA polymerase II to transcribe the DNA in mRNA. We developed a single pair Förster Resonance Energy Transfer (spFRET) assay to investigate the mechanism of gene regulation. Here, we apply this assay to investigate the initial binding process of TBP to the adenovirus major late (AdML) promoter site. From the spFRET measurements, we were able to identify two conformations of the TBP-DNA complex that correspond to TBP bound in the correct and the opposite orientation. Increased incubation times or the presence of the transcription factor TFIIA improved the alignment of TBP on the promoter site. Binding of TBP to the TATA box shows a rich dynamics with abrupt transitions between multiple FRET states. A frame-wise histogram analysis revealed the presence of at least six discrete states, showing that TBP binding is more complicated than previously thought. Hence, the spFRET assay is very sensitive to the conformation of the TBP-DNA complex and is very promising tool for investigating the pathway of TBP binding in detail.

  7. The Role of Y-Box Binding Protein 1 in Kidney Injury: Friend or Foe?

    Science.gov (United States)

    Ke, Ben; Fan, Chuqiao; Tu, Weiping; Fang, Xiangdong

    2018-01-01

    Y-box-binding protein 1 (YB-1) is a multifunctional protein involved in various cellular processes via the transcriptional and translational regulation of target gene expression. YB-1 promotes acute or chronic kidney injury through multiple molecular pathways; however, accumulating evidence suggests that significantly increased YB-1 levels are of great importance in renoprotection. In addition, YB-1 may contribute to obesity-related kidney disease by promoting adipogenesis. Thus, the role of YB-1 in kidney injury is complicated, and no comprehensive review is currently available. In this review, we summarise recent progress in our understanding of the function of YB-1 in kidney injury and provide an overview of the dual role of YB-1 in kidney disease. Moreover, we propose that YB-1 is a potential therapeutic target to restrict kidney disease. © 2018 The Author(s). Published by S. Karger AG, Basel.

  8. Trust Ownership of the Tata Group

    DEFF Research Database (Denmark)

    Thomsen, Steen

    The Indian Tata Group is one of the largest and most admired business groups in the world. It has 28 listed subsidiaries and more than 80 operating businesses. It has shown strong financial performance and social responsibility for decades. Interestingly, it has a unique ownership structure......: the main holding company Tata Sons Limited is majority-owned by charitable trusts. We examine the governance of this remarkable entity. The Trusts own 66% of Tata Sons, the main holding company of the Group, while members of the founding Tata family are very small minority shareholders. The governance...... structure is characterized by managerial distance between trusts and Group companies. The Trusts are almost exclusively concerned with philanthropy, and according to the Articles of Association of Tata Sons Limited, their governance role is limited to nominating two members to the Selection Committee which...

  9. The Y-Box Binding Protein 1 Suppresses Alzheimer's Disease Progression in Two Animal Models.

    Directory of Open Access Journals (Sweden)

    N V Bobkova

    Full Text Available The Y-box binding protein 1 (YB-1 is a member of the family of DNA- and RNA binding proteins. It is involved in a wide variety of DNA/RNA-dependent events including cell proliferation and differentiation, stress response, and malignant cell transformation. Previously, YB-1 was detected in neurons of the neocortex and hippocampus, but its precise role in the brain remains undefined. Here we show that subchronic intranasal injections of recombinant YB-1, as well as its fragment YB-11-219, suppress impairment of spatial memory in olfactory bulbectomized (OBX mice with Alzheimer's type degeneration and improve learning in transgenic 5XFAD mice used as a model of cerebral amyloidosis. YB-1-treated OBX and 5XFAD mice showed a decreased level of brain β-amyloid. In OBX animals, an improved morphological state of neurons was revealed in the neocortex and hippocampus; in 5XFAD mice, a delay in amyloid plaque progression was observed. Intranasally administered YB-1 penetrated into the brain and could enter neurons. In vitro co-incubation of YB-1 with monomeric β-amyloid (1-42 inhibited formation of β-amyloid fibrils, as confirmed by electron microscopy. This suggests that YB-1 interaction with β-amyloid prevents formation of filaments that are responsible for neurotoxicity and neuronal death. Our data are the first evidence for a potential therapeutic benefit of YB-1 for treatment of Alzheimer's disease.

  10. Spliced X-box binding protein 1 couples the unfolded protein response to hexosamine biosynthetic pathway.

    Science.gov (United States)

    Wang, Zhao V; Deng, Yingfeng; Gao, Ningguo; Pedrozo, Zully; Li, Dan L; Morales, Cyndi R; Criollo, Alfredo; Luo, Xiang; Tan, Wei; Jiang, Nan; Lehrman, Mark A; Rothermel, Beverly A; Lee, Ann-Hwee; Lavandero, Sergio; Mammen, Pradeep P A; Ferdous, Anwarul; Gillette, Thomas G; Scherer, Philipp E; Hill, Joseph A

    2014-03-13

    The hexosamine biosynthetic pathway (HBP) generates uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) for glycan synthesis and O-linked GlcNAc (O-GlcNAc) protein modifications. Despite the established role of the HBP in metabolism and multiple diseases, regulation of the HBP remains largely undefined. Here, we show that spliced X-box binding protein 1 (Xbp1s), the most conserved signal transducer of the unfolded protein response (UPR), is a direct transcriptional activator of the HBP. We demonstrate that the UPR triggers HBP activation via Xbp1s-dependent transcription of genes coding for key, rate-limiting enzymes. We further establish that this previously unrecognized UPR-HBP axis is triggered in a variety of stress conditions. Finally, we demonstrate a physiologic role for the UPR-HBP axis by showing that acute stimulation of Xbp1s in heart by ischemia/reperfusion confers robust cardioprotection in part through induction of the HBP. Collectively, these studies reveal that Xbp1s couples the UPR to the HBP to protect cells under stress. Copyright © 2014 Elsevier Inc. All rights reserved.

  11. [Roles of Y box-binding protein 1 in SK-BR-3 breast cancer proliferation].

    Science.gov (United States)

    Shi, Jianhong; Lü, Xinrui; Wang, Bing; Daudan, Lin; Yanan, Wang; Yuhui, Bu; Zhenfeng, Ma

    2014-09-30

    To explore the roles of Y box-binding protein 1 (YB-1) in breast cancer cell proliferation. Twenty cases of surgical removal of breast cancer tissue (diagnosed with invasive ductal carcinoma, stage II, by postoperative paraffin pathology) and normal breast tissues adjacent to carcinoma were collected during June 2013 to August 2013.Quantitative real-time PCR (qRT-PCR) was performed to detect the YB1 mRNA levels. Cultured mammary epithelial cells (HBL-100) and breast cancer cells (MCF7, MDA-MB-231 & SK-BR-3 cells) were harvested and qRT-PCR was performed to detect the YB1 mRNA levels.SK-BR-3 cells were stimulated with various concentrations of PDGF-BB and YB1 expression levels were detected by qRT-PCR. Down-regulation or over-expression of YB1 by si-YB1 or Ad-GFP-YB1 was detected in SK-BR-3 cells. And MTS cell proliferation assay kit was used to detect cell proliferation. YB1 mRNA levels were significantly higher in breast cancer tissues and MDA-MB-231 and SK-BR-3 breast cancer cell lines than that in adjacent normal breast tissues and HBL-100 mammary epithelial cells respectively (P BR-3 cells in a dose-dependent manner. A down-regulation of endogenous YB1 decreases and an over-expression of exogenous YB1 promotes the proliferation activity in SK-BR-3 cells.

  12. Mutations on the DNA binding surface of TBP discriminate between yeast TATA and TATA-less gene transcription.

    Science.gov (United States)

    Kamenova, Ivanka; Warfield, Linda; Hahn, Steven

    2014-08-01

    Most RNA polymerase (Pol) II promoters lack a TATA element, yet nearly all Pol II transcription requires TATA binding protein (TBP). While the TBP-TATA interaction is critical for transcription at TATA-containing promoters, it has been unclear whether TBP sequence-specific DNA contacts are required for transcription at TATA-less genes. Transcription factor IID (TFIID), the TBP-containing coactivator that functions at most TATA-less genes, recognizes short sequence-specific promoter elements in metazoans, but analogous promoter elements have not been identified in Saccharomyces cerevisiae. We generated a set of mutations in the yeast TBP DNA binding surface and found that most support growth of yeast. Both in vivo and in vitro, many of these mutations are specifically defective for transcription of two TATA-containing genes with only minor defects in transcription of two TATA-less, TFIID-dependent genes. TBP binds several TATA-less promoters with apparent high affinity, but our results suggest that this binding is not important for transcription activity. Our results are consistent with the model that sequence-specific TBP-DNA contacts are not important at yeast TATA-less genes and suggest that other general transcription factors or coactivator subunits are responsible for recognition of TATA-less promoters. Our results also explain why yeast TBP derivatives defective for TATA binding appear defective in activated transcription. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

  13. DESAIN, TATA LETAK, DAN KONSTRUKSI TAMBAK

    Directory of Open Access Journals (Sweden)

    Akhmad Mustafa

    2008-12-01

    Full Text Available Komoditas yang umum dibudidayakan di tambak Indonesia adalah udang dan ikan bandeng yang menjadi komoditas unggulan untuk dikembangkan. Salah satu faktor penting yang sangat menentukan keberhasilan usaha budidaya tambak adalah rekayasa tambak yang mencakup disain, tata letak, dan konstruksi tambak. Secara umum, desain petakan tambak merupakan perencanaan bentuk tambak yang meliputi: ukuran panjang dan lebar petakan, kedalaman, ukuran pematang, ukuran berm, dan ukuran saluran keliling, serta ukuran dan letak pintu air. Tata letak suatu unit tambak harus memenuhi tujuan seperti: menjamin kelancaran mobilitas operasional sehari-hari, menjamin kelancaran dan keamanan pasok air, serta pembuangannya, dapat menekan biaya konstruksi tanpa mengurangi fungsi teknis dari unit tambak yang dibangun dan mempertahankan kelestarian lingkungan. Konstruksi tambak yang menggambarkan proses pengerjaan tambak harus disesuaikan dengan desain dan tata letak yang telah ada. Rekayasa tambak diarahkan pada kemampuan untuk menciptakan kondisi yang sesuai dengan keadaan alami yang dituntut oleh organisme akuatik yang dibudidayakan sehingga produktivitas tambak meningkat, efisien secara ekonomis, dan berkelanjutan.

  14. Menanamkan Konsep Tata Krama pada Anak melalui Perancangan Game Edukasi

    Directory of Open Access Journals (Sweden)

    Dea Faustina Shaula

    2017-09-01

    Full Text Available Tata krama sangat berperan penting untuk mepermudah manusia untuk diterima di lingkungan masyarakat sekitar. Peran orang tua sangatlah penting dalam pembentukan karakter anak terutama dalam menerapkan sikap tata krama atau sopan santun anak. . Saat ini perilaku anak lebih cenderung kehilangan etika dan sopan santun. Berdasarkan obeservasi yang dilakukan di beberapa sekolah dasar di Semarang ditemui beberapa sikap yang mencerminkan kurangnya tata krama siswa di sekolah. Perlu adanya media yang kreatif untuk menanmkan tata krama kepada anak dengan melibatkan perkembangan teknologi, salah satunya melalui perancanan game edukasi berbasis mobile. Pada penelitian ini,metode yang digunakan adalah metode pengembangan multimedia yang meliputi membuat konsep (concept, merancang desain (design, mengumpulkan bahan (material collecting, pembuatan (assembly, melakukan pengujian (testing, dan evaluasi produk (evaluating. Desain game dibuat desain vektor dengan style karakter chibi yang memiliki ciri kepala besar dan badan mungil atau kecil untuk membuat karakter lebih terlihat lucu. Hasil perancangan ini adalah sebuah game edukasi untuk menanamkan tata krama pada anak.

  15. Expression of zinc finger E-box-binding homeobox factor 1 in epithelial ovarian cancer: A clinicopathological analysis of 238 patients

    OpenAIRE

    LI, XIUFANG; HUANG, RUIXIA; LI, RUTH HOLM; TROPE, CLAES G.; NESLAND, JAHN M.; SUO, ZHENHE

    2015-01-01

    A growing body of evidence indicates that aberrant activation of epithelial-to-mesenchymal transition (EMT) plays a key role in tumor cell invasion and metastasis. Zinc finger E-box-binding homeobox factor 1 (ZEB1), as a crucial mediator of EMT, contributes to the malignant progression of various epithelial tumors. To determine whether ZEB1 is involved in the progression of ovarian cancer, we immunohistochemically evaluated the expression of ZEB1 in 238 cases of epithelial ovarian cancer (EOC...

  16. In silico studies on structure-function of DNA GCC- box binding domain of brassica napus DREB1 protein

    International Nuclear Information System (INIS)

    Qamarunnisa, S.; Hussain, M.

    2012-01-01

    DREB1 is a transcriptional factor, which selectively binds with the promoters of the genes involved in stress response in the plants. Homology of DREB protein and its binding element have been detected in the genome of many plants. However, only a few reports exist that discusses the binding properties of this protein with the gene (s) promoter. In the present study, we have undertaken studies exploring the structure-function relationship of Brassica napus DREB1. Multiple sequence alignment, protein homology modeling and intermolecular docking of GCC-box binding domain (GBD) of the said protein was carried out using atomic coordinates of GBD from Arabdiopsis thaliana and GCC-box containing DNA respectively. Similarities and/or identities in multiple, sequence alignment, particularly at the functionally important amino acids, strongly suggested the binding specificity of B. napus DREB1 to GCC-box. Similarly, despite 56% sequence homology, tertiary structures of both template and modeled protein were found to be extremely similar as indicated by root mean square deviation of 0.34 A. More similarities were established between GBD of both A. thaliana and B. napus DREB1 by conducting protein docking with the DNA containing GCC-box. It appears that both proteins interact through their beta-sheet with the major DNA groove including both nitrogen bases and phosphate and sugar moieties. Additionally, in most cases the interacting residues were also found to be identical. Briefly, this study attempts to elucidate the molecular basis of DREB1 interaction with its target sequence in the promoter. (author)

  17. Functional characterization of the ER stress induced X-box-binding protein-1 (Xbp-1 in the porcine system

    Directory of Open Access Journals (Sweden)

    Jin Dong-Il

    2011-05-01

    Full Text Available Abstract Background The unfolded protein response (UPR is an evolutionary conserved adaptive reaction for increasing cell survival under endoplasmic reticulum (ER stress conditions. X-box-binding protein-1 (Xbp1 is a key transcription factor of UPR that activates genes involved in protein folding, secretion, and degradation to restore ER function. The UPR induced by ER stress was extensively studied in diseases linked to protein misfolding and aggregations. However, in the porcine system, genes in the UPR pathway were not investigated. In this study, we isolated and characterized the porcine Xbp1 (pXbp1 gene in ER stress using porcine embryonic fibroblast (PEF cells and porcine organs. ER stress was induced by the treatment of tunicamycin and cell viability was investigated by the MTT assay. For cloning and analyzing the expression pattern of pXbp1, RT-PCR analysis and Western blot were used. Knock-down of pXbp1 was performed by the siRNA-mediated gene silencing. Results We found that the pXbp1 mRNA was the subject of the IRE1α-mediated unconventional splicing by ER stress. Knock-down of pXbp1 enhanced ER stress-mediated cell death in PEF cells. In adult organs, pXbp1 mRNA and protein were expressed and the spliced forms were detected. Conclusions It was first found that the UPR mechanisms and the function of pXbp1 in the porcine system. These results indicate that pXbp1 plays an important role during the ER stress response like other animal systems and open a new opportunity for examining the UPR pathway in the porcine model system.

  18. The zinc finger E-box-binding homeobox 1 (Zeb1) promotes the conversion of mouse fibroblasts into functional neurons.

    Science.gov (United States)

    Yan, Long; Li, Yue; Shi, Zixiao; Lu, Xiaoyin; Ma, Jiao; Hu, Baoyang; Jiao, Jianwei; Wang, Hongmei

    2017-08-04

    The zinc finger E-box-binding transcription factor Zeb1 plays a pivotal role in the epithelial-mesenchymal transition. Numerous studies have focused on the molecular mechanisms by which Zeb1 contributes to this process. However, the functions of Zeb1 beyond the epithelial-mesenchymal transition remain largely elusive. Using a transdifferentiation system to convert mouse embryonic fibroblasts (MEFs) into functional neurons via the neuronal transcription factors achaete-scute family bHLH (basic helix-loop-helix) transcription factor1 ( Ascl1 ), POU class 3 homeobox 2 (POU3F2/ Brn2 ), and neurogenin 2 (Neurog2, Ngn2 ) (ABN), we found that Zeb1 was up-regulated during the early stages of transdifferentiation. Knocking down Zeb1 dramatically attenuated the transdifferentiation efficiency, whereas Zeb1 overexpression obviously increased the efficiency of transdifferentiation from MEFs to neurons. Interestingly, Zeb1 improved the transdifferentiation efficiency induced by even a single transcription factor ( e.g. Asc1 or Ngn2 ). Zeb1 also rapidly promoted the maturation of induced neuron cells to functional neurons and improved the formation of neuronal patterns and electrophysiological characteristics. Induced neuron cells could form functional synapse in vivo after transplantation. Genome-wide RNA arrays showed that Zeb1 overexpression up-regulated the expression of neuron-specific genes and down-regulated the expression of epithelial-specific genes during conversion. Taken together, our results reveal a new role for Zeb1 in the transdifferentiation of MEFs into neurons. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

  19. Building the Method to Determine the Rate of Freezing Water in Penaeus monodon of the Freezing Process

    OpenAIRE

    Nguyen Tan Dzung; Trinh Van Dzung; Tran Duc Ba

    2012-01-01

    The method of determination the rate of freezing water in Penaeus monodon of freezing process was established on base the equation of energy balance in warming up process Penaeus monodon after freezing to determine specific heat of Penaeus monodon. The result obtained was built the mathematical model (19) to determine the rate of freezing water according to the freezing temperature of Penaeus monodon. The results indicated that when water was completely frozen (ω = 1 or 100%), the optimal fre...

  20. Homi Bhabha Centre for Science Education, Tata Institute of ...

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 22; Issue 2. Homi Bhabha Centre for Science Education, Tata Institute of Fundamental Research (A Deemed University). Information and Announcements Volume 22 Issue 2 February 2017 pp 189-189 ...

  1. Homi Bhabha Centre for Science Education Tata Institute of ...

    Indian Academy of Sciences (India)

    Tata Institute of Fundamental Research (A Deemed University) ... level • Innovative curricula, laboratories, teaching and assessment methods • Development ... subject), M Tech or a Master's degree (MA/MSW) in any of the social/ behavioural.

  2. Influence of vaccination on vibriosis resistance of the giant black tiger shrimp Peneaus monodon (Fabricius).

    NARCIS (Netherlands)

    Teunissen, O.S.P.; Faber, R.; Booms, G.H.R.; Latscha, T.; Boon, J.H.

    1998-01-01

    The study aims to test the influence of vaccination with polyvalent vaccine prototypes on the vibriosis resistance of Penaeus monodon. Vaccinated P. monodon post-larvae were challenged with a virulent Vibrio alginolyticus strain 10, 20, and 30 days post-vaccination. Results showed that vaccination

  3. Effects of crushed conspecifics on growth and survival of Penaeus monodon Fabricius post larvae

    NARCIS (Netherlands)

    Nga, B.T.; Lürling, M.F.L.L.W.; Peeters, E.T.H.M.; Roijackers, R.M.M.; Scheffer, M.; Nghia, T.T.

    2006-01-01

    In the present study, the hypothesis tested was that Penaeus monodon post-larvae (PL) experience lower growth when exposed to crushed conspecifics, which was achieved by exposing individual P. monodon PL with abundant food for 4 weeks to a gradient from 0 to 100 crushed conspecific PL L1. Both dry

  4. Structural basis for TatA oligomerization: an NMR study of Escherichia coli TatA dimeric structure.

    Directory of Open Access Journals (Sweden)

    Yi Zhang

    Full Text Available Many proteins are transported across lipid membranes by protein translocation systems in living cells. The twin-arginine transport (Tat system identified in bacteria and plant chloroplasts is a unique system that transports proteins across membranes in their fully-folded states. Up to date, the detailed molecular mechanism of this process remains largely unclear. The Escherichia coli Tat system consists of three essential transmembrane proteins: TatA, TatB and TatC. Among them, TatB and TatC form a tight complex and function in substrate recognition. The major component TatA contains a single transmembrane helix followed by an amphipathic helix, and is suggested to form the translocation pore via self-oligomerization. Since the TatA oligomer has to accommodate substrate proteins of various sizes and shapes, the process of its assembly stands essential for understanding the translocation mechanism. A structure model of TatA oligomer was recently proposed based on NMR and EPR observations, revealing contacts between the transmembrane helices from adjacent subunits. Herein we report the construction and stabilization of a dimeric TatA, as well as the structure determination by solution NMR spectroscopy. In addition to more extensive inter-subunit contacts between the transmembrane helices, we were also able to observe interactions between neighbouring amphipathic helices. The side-by-side packing of the amphipathic helices extends the solvent-exposed hydrophilic surface of the protein, which might be favourable for interactions with substrate proteins. The dimeric TatA structure offers more detailed information of TatA oligomeric interface and provides new insights on Tat translocation mechanism.

  5. Tata võidujooks Jaguarile ja Roverile / Gail Edmondson, David Welch

    Index Scriptorium Estoniae

    Edmondson, Gail

    2007-01-01

    India autotöösturile Ratan N. Tata kuuluv Tata Motors soovib osta kahjumis olevad Jaguar Carsi ja Land Roveri, mille Ford Motor on enampakkumisele pannud. Kaasautor Nandini Lakshman. Diagramm: Briti brändidest kaugel ees

  6. Aspek Budaya Dalam Keistimewaan Tata Ruang Kota Yogyakarta

    Directory of Open Access Journals (Sweden)

    Suryanto .

    2015-09-01

    Full Text Available Dengan ditetapkannya budaya dan tata ruang kota sebagai penanda keistimewaan Yogyakarta dalam UU No. 13 tahun 2012 tentang Keistimewaan Yogyakarta, maka Yogyakarta merupakan kasus spesifik dalam Penataan Ruang, karena aspek Tata Ruang menjadi salah satu penanda Keistimewaannya. Apa yang istimewa. Apanya yang istimewa dang mengapa istimewa; apakah penanda (tata ruang kota, petanda (konsep budaya atau makna/pesan dari hubungan antara petanda dengan penandanya yang tersirat dalam wujud tata ruang kota Yogya. Penelitian ini berupaya untuk mengenali dan memahami hubungan antara kebudayaan, tata kota dan keistimewaan Yogyakarta. Dari bukti-bukti empiris, kajian tentang kebudayan dan tata ruang kota memerlukan rentang waktu panjang, karena akan menyangkut data longitudinal (diakronik dan lateral (sinkronik. Oleh karena itu studi ini perlu didudukkan dalam bingkai sejarah dan budaya, untuk membaca peristiwa sepanjang perkembangan kota Yogyakarta, dari HB I sampai HB IX. Kemudian untuk memahami makna kaitan antar penanda dan petanda sepanjang perjalanan perkembangan kota, maka digunakan metoda hermeneutika, khususnya Hermeneutik Paul Ricoeur. Dari hasil kajian terhadap obyek tata ruang kota yang dianggap istimewa, maka budaya yang mewujud dalam keistimewaan tata ruang kota Yogyakarta bisa dilihat dari komponen ruang kotanya maupun konfigurasi fungsi ruang kotanya. Kesimpulan penting dari penelitian ini adalah konsep budaya yang mewujud dalam tata ruang kota, yaitu monumental dan pertahanan, yang tidak ditemui di kota manapun di Indonesia. Kemudian dari sisi makna, terjadi perbedaan makna simbol-simbol tata ruang kota di era HB I dan HB IX.Kata kunci. Kebudayaan, tata ruang kota, budaya, Yogyakarta. Since Yogyakarta’s culture and spatiality were proclaimed as special features of Yogyakarta as stated in Law No. 13, 2012 on the Special Region of Yogyakarta, Yogyakarta is recognized as a specific case in spatial planning, because its spatiality is one of

  7. Current status of iron and steelmaking technology at Tata Steel

    Energy Technology Data Exchange (ETDEWEB)

    Pandey, B.D.; Poddar, M.N.; Chandra, S. [Tata Steel, Jamshedpur (India)

    2002-07-01

    Tata Steel was set up in the early years of the 20th century and over the years the plant has grown into one of the most modern steel plants in the world. The philosophy of phase-wise modernisation on a continuing basis was adopted by Tata Steel with great advantage for the modernisation of the two million tonne Jamshedpur Steel Works. Four phases of the modernisation programme have already been successfully completed and their gains consolidated. Adoption and absorption of the latest technologies, fundamental changes in the operating philosophy and setting of stretch performance targets have brought about this remarkable transformation. The recently commissioned state of the art 1.2 Mtpa Cold Rolling Mill Complex is an example of Tata Steel remaining in harmony with times. Another is the use of pulverised coal injection in blast furnaces. The paper highlights some of the important technological developments in integrated steel plants, particularly those being practiced at Tata Steel, in the areas of ironmaking, steelmaking, casting and rolling for retaining its competitive position in the global market with regard to cost, customer and change. 9 refs., 21 figs., 2 tabs.

  8. molecular interactions of the TATA-binding protein

    Indian Academy of Sciences (India)

    Unknown

    the recruitment and delivery of TBP on to the promoter, a step shown to be ..... giving an increase in protection in the presence of GAL4-. VP16 (figure 2B,C). ...... minor groove surface of the TATA box unwound by TBP;. Cell 94 573–583.

  9. Length and weight relationship of laboratory reared penaeid prawn Penaeus monodon (Fabricius) (Crustacea: Penaeidae)

    Digital Repository Service at National Institute of Oceanography (India)

    Saldanha, C.M.; Chatterji, A.

    A parabolic relationship was obtained while studying the length-weight relationship of the postlarvae of Penaeus monodon. The b value (multiplying constant) showed that in the laboratory reared penaeid prawns, length-weight relationship strictly...

  10. Fungal infection in the tiger prawn (Penaeus monodon) and in other crustaceans from the Cochin Backwaters

    Digital Repository Service at National Institute of Oceanography (India)

    Gopalan, U.K.; Meenakshikunjamma, P.P.; Purushan, K.S.

    Infection of the two species of fungi (Phycomycetes) in the tiger prawn (Penaeus monodon) and three species in amphipods (Gammarus sp.) and tanaeidaceans (Apseudes sp.) was observed. Of the two pathogens in the shrimp, Leptolegnia marina produced...

  11. The integrated endoplasmic reticulum stress response in Leishmania amazonensis macrophage infection: the role of X-box binding protein 1 transcription factor.

    Science.gov (United States)

    Dias-Teixeira, Karina Luiza; Calegari-Silva, Teresa Cristina; dos Santos, Guilherme R R M; Vitorino Dos Santos, José; Lima, Carolina; Medina, Jorge Mansur; Aktas, Bertal Huseyin; Lopes, Ulisses G

    2016-04-01

    Endoplasmic reticulum (ER) stress triggers the integrated ER-stress response (IERSR) that ensures cellular survival of ER stress and represents a primordial form of innate immunity. We investigated the role of IERSR duringLeishmania amazonensisinfection. Treatment of RAW 264.7 infected macrophages with the ER stress-inducing agent thapsigargin (TG; 1 μM) increasedL. amazonensisinfectivity in an IFN1-α receptor (IFNAR)-dependent manner. In Western blot assays, we showed thatL. amazonensisactivates the inositol-requiring enzyme (IRE1)/ X-box binding protein (XBP)-1-splicing arms of the IERSR in host cells. In chromatin immunoprecipitation (ChIP) assays, we showed an increased occupancy of enhancer and promoter sequences for theIfnbgene by XBP1 in infected RAW 264.7 cells. Knocking down XBP1 expression by transducing RAW 264.7 cells with the short hairpin XBP1 lentiviral vector significantly reduced the parasite proliferation associated with impaired translocation of phosphorylated IFN regulatory transcription factor (IRF)-3 to the nucleus and a decrease in IFN1-β expression. Knocking down XBP1 expression also increased NO concentration, as determined by Griess reaction and reduced the expression of antioxidant genes, such as heme oxygenase (HO)-1, that protect parasites from oxidative stress. We conclude thatL. amazonensisactivation of XBP1 plays a critical role in infection by protecting the parasites from oxidative stress and increasing IFN1-β expression.-Dias-Teixeira, K. L., Calegari-Silva, T. C., Dos Santos, G. R. R. M., Vitorino dos Santos, J., Lima, C., Medina, J. M., Aktas, B. H., Lopes, U. G. The integrated endoplasmic reticulum stress response inLeishmania amazonensismacrophage infection: the role of X-box binding protein 1 transcription factor. © FASEB.

  12. Life history parameters of narwhals (Monodon monoceros) from Greenland

    DEFF Research Database (Denmark)

    Garde, Eva; Hansen, Steen H; Ditlevsen, Susanne

    2015-01-01

    Life history parameters for narwhals (Monodon monoceros) were estimated based on age estimates from aspartic acid racemization of eye lens nuclei. Eyes, reproductive organs, and measures of body lengths were collected from 282 narwhals in East and West Greenland in the years 1993, 2004, and 2007–...... projection matrix was parameterized with the data on age structure and fertility rates. The annual rate of increase of narwhals in East Greenland was estimated to be 3.8% while narwhals in West Greenland had a rate of increase at 2.6%. Key words:......Life history parameters for narwhals (Monodon monoceros) were estimated based on age estimates from aspartic acid racemization of eye lens nuclei. Eyes, reproductive organs, and measures of body lengths were collected from 282 narwhals in East and West Greenland in the years 1993, 2004, and 2007......–2010. Age estimates were based on the racemization of L-aspartic acid to D-aspartic acid in the nucleus of the eye lens. The ratio of D- and L-enantiomers was measured using high-performance liquid chromatography. The age equation used, 420.32X − 24.02·year where X is the D/L ratio, was determined from data...

  13. Haldia complex in doubt as Tata pulls out

    International Nuclear Information System (INIS)

    Alperowicz, N.

    1993-01-01

    The Tata Tea group (Calcutta) intends to pull out of Haldia Petrochemicals, casting a shadow on India's second major petrochemical project. Late last year Shell withdrew from National Organic Chemical Industries (Nocil; Bombay), throwing Nocil's plans into disarray. Tata - copromoting Halida with the West Bengal government - cites escalating costs as the main reason for its decision. The Haldia project was revised in 1990 to have annual capacities for 300,000 m.t. of ethylene, 100,000 m.t. of high-density polyethylene (HDPE), 160,000 m.t. of linear low-density polyethylene, and 150,000 m.t. of polypropylene (PP). The cost was frozen at Rs30 billion, but the exchange rate shifted and the petrochemical markets suffered a downturn. Lummus Crest has been shortlisted to supply the ethylene plant, with Mitsui for HDPE and Himont for PP

  14. Medical applications of accelerators at Tata Memorial Centre

    International Nuclear Information System (INIS)

    Dinshaw, K.A.

    2003-01-01

    The Tata Memorial Centre constitutes the national comprehensive cancer centre for the prevention, diagnosis, treatment and research on cancer. It is well equipped with sophisticated state-of-the-art equipment capable of delivering External Beam Radiotherapy (Ebert) and Brachytherapy. Nearly 400 patients receive Ebert daily at the institute from a team of highly skilled and dedicated radiation oncologists, medical physicists and technologists, making it one of the busiest centres in the country

  15. Tata Laksana Non Imunosupresan Sindrom Nefrotik pada Anak

    Directory of Open Access Journals (Sweden)

    Sudung Oloan Pardede

    2017-08-01

    Full Text Available Sindrom nefrotik adalah keadaan klinis yang terdiri atas proteinuria masif, hipo­albuminemia (< 2,5 g/dL, edema, dan hiperkolesterolemia. Terapi utama sindrom nefrotik adalah imunosupresan terutama kortikosteroid. Pada sindrom nefrotik relaps sering atau dependen steroid, dan sindrom nefrotik resisten steroid, selain steroid diberikan juga imunosupresan lain seperti siklofosfamid, siklosporin, mikofenolat mofetil, takrolimus, atau pun levamisol. Selain pemberian imunosupresan diperlukan terapi suportif, yang meliputi terapi diitetik,  tata laksana edema, hipertensi, hipovolemia, trombosis, hiperlipidemia, dan infeksi. Tata laksana diitetik terdiri atas kalori yang adekuat, protein sesuai recommended daily allowance, lemak low saturated, dan rendah garam. Komposisi zat gizi yang dianjurkan terdiri atas 10-14% protein; 40-50% lemak poly- dan monounsaturated, 40-50% karbohidrat. Tata laksana  edema terdiri atas restriksi cairan, pemberian diuretik, dan infus albumin jika perlu. Infeksi yang sering terjadi pada sindrom nefrotik adalah selulitis peritonitis, dan pneumonia yang diterapi dengan antibiotik sefotaksim, seftriakson, ko-amoksiklav. Antihipertensi yang digunakan pada anak umumnya diuretik, angiotensin converting enzyme inhibitors, angiotensin receptor blockers, calcium channel blockers, (amblodipin, nifedipin, isradipin, alpha-symphatetic agents, beta blockers dan vasodilator. Aktivitas fisik tidak perlu dibatasi, dan pada edema ringan atau tidak berat tidak perlu dilarang pergi ke sekolah

  16. Nuclear factor ETF specifically stimulates transcription from promoters without a TATA box.

    Science.gov (United States)

    Kageyama, R; Merlino, G T; Pastan, I

    1989-09-15

    Transcription factor ETF stimulates the expression of the epidermal growth factor receptor (EGFR) gene which does not have a TATA box in the promoter region. Here, we show that ETF recognizes various GC-rich sequences including stretches of deoxycytidine or deoxyguanosine residues and GC boxes with similar affinities. ETF also binds to TATA boxes but with a lower affinity. ETF stimulated in vitro transcription from several promoters without TATA boxes but had little or no effect on TATA box-containing promoters even though they had strong ETF-binding sites. These inactive ETF-binding sites became functional when placed upstream of the EGFR promoter whose own ETF-binding sites were removed. Furthermore, when a TATA box was introduced into the EGFR promoter, the responsiveness to ETF was abolished. These results indicate that ETF is a specific transcription factor for promoters which do not contain TATA elements.

  17. Monoclonal antibodies against haemocyte molecules of Penaeus monodon shrimp react with haemolymph components of other crustaceans and disparate taxa

    NARCIS (Netherlands)

    Braak, van de C.B.T.; Botterblom, M.H.A.; Taverne, N.; Knaap, van der W.P.W.; Rombout, J.H.W.M.

    2001-01-01

    In a previous study, monoclonal antibodies (mAbs) against different haemolymph molecules of the marine shrimp Penaeus monodon were produced and characterised. It was suggested that these mAbs could be used in studying haemocyte differentiation, behaviour and function in P. monodon. In the present

  18. Efektifitas Dukungan Sosial Dokter kepada Orangtua dalam Tata Laksana Anak Asma

    Directory of Open Access Journals (Sweden)

    Fx. Wikan Indrarto

    2016-11-01

    Kesimpulan. Pemberian dukungan sosial dokter tidak berhubungan dengan keberhasilan tata laksana anak asma. Kepuasan orangtua akan pemberian dukungan sosial dokter, terutama diperoleh dari jenis dukungan penghargaan.

  19. ANALISIS KUALITAS PELAYANAN PADA BAGIAN TATA USAHA BERDASARKAN TINGKAT KEPUASAN MAHASISWA

    Directory of Open Access Journals (Sweden)

    Muzakiyah Muzakiyah

    2011-12-01

    Full Text Available Kepuasan pelanggan merupakan hasil (outcome yang dirasakan atas penggunanya produk atau jasa, sama atau melebihi harapan yang diinginkan. Pelayanan di bagian Tata Usaha idealnya lebih bersahabat, cepat dan akurat. Ini berarti orientasi layanan di bagian Tata Usaha harus didasarkan pada kebutuhan mahasiswa. Dalam penelitian ini metode yang digunakan dengan menggunakan Pendekatan Lean ServPerf (Lean Service dan Service Performance. Service Performance digunakan untuk menganalisis tingkat instrumen kinerja pelayanan bagian Tata Usaha. Lean Service digunakan untuk menghilangkan waste aktivitas yang tidak bernilai tambah. Hasil dari penelitian ini, terdapat 14 atribut pelayanan yang perlu dilakukan perbaikan untuk meningkatkan kualitas pelayanan di bagian Tata Usaha Fakultas.

  20. Inhibition of Y-box binding protein-1 slows the growth of glioblastoma multiforme and sensitizes to temozolomide independent O6-methylguanine-DNA methyltransferase.

    Science.gov (United States)

    Gao, Yuanyuan; Fotovati, Abbas; Lee, Cathy; Wang, Michelle; Cote, Gilbert; Guns, Emma; Toyota, Brian; Faury, Damien; Jabado, Nada; Dunn, Sandra E

    2009-12-01

    Glioblastoma multiforme (GBM) is an aggressive type of brain tumor where 5 years. In adults, GBM is the most common type of brain tumor. It is rarer in children, where it constitutes approximately 15% of all brain tumors diagnosed. These tumors are often invasive, making surgical resection difficult. Further, they can be refractory to current therapies such as temozolomide. The current dogma is that temozolomide resistance rests on the expression of O6-methylguanine-DNA methyltransferase (MGMT) because it cleaves methylated DNA adducts formed by the drug. Our laboratory recently reported that another drug resistance gene known as the Y-box binding protein-1 (YB-1) is highly expressed in primary GBM but not in normal brain tissues based on the evaluation of primary tumors. We therefore questioned whether GBM depend on YB-1 for growth and/or response to temozolomide. Herein, we report that YB-1 inhibition reduced tumor cell invasion and growth in monolayer as well as in soft agar. Moreover, blocking this protein ultimately delayed tumor onset in mice. Importantly, inhibiting YB-1 enhanced temozolomide sensitivity in a manner that was independent of MGMT in models of adult and pediatric GBM. In conclusion, inhibiting YB-1 may be a novel way to improve the treatment of GBM.

  1. YB1/p32, a nuclear Y-box binding protein 1, is a novel regulator of myoblast differentiation that interacts with Msx1 homeoprotein

    Energy Technology Data Exchange (ETDEWEB)

    Song, Young Joon [Department of Biological Sciences, College of Natural Science, Inha University, 253 Yonghyun-dong, Nam-Gu, Incheon, Korea, 402-751 (Korea, Republic of); Lee, Hansol, E-mail: hlee@inha.ac.kr [Department of Biological Sciences, College of Natural Science, Inha University, 253 Yonghyun-dong, Nam-Gu, Incheon, Korea, 402-751 (Korea, Republic of)

    2010-02-15

    Precisely controlled cellular differentiation is essential for the proper development of vertebrate embryo and deregulated differentiation is a major cause of many human congenital diseases as well as cancer. Msx1 is a member of the homeoprotein family implicated in these processes, which inhibits the differentiation of skeletal muscle and other cell types, presumably by regulating transcription of target genes through interaction with other cellular factors. We presently show that YB1/p32, a nuclear Y-box binding protein 1, interacts with Msx1 homeoprotein and functions as a regulator of C2C12 myoblast differentiation. We demonstrate that YB1/p32 functionally interacts with Msx1 through its N-terminal region and colocalizes with Msx1 at the nuclear periphery. Moreover, we find that YB1/p32 is competent for inhibition of C2C12 myoblast differentiation, which is correlated with its activity as a negative regulator of MyoD gene expression and binding to the MyoD core enhancer region (CER). Furthermore, YB1/p32 cooperates with Msx1 in transcriptional repression and knocking down the expression of endogenous YB1 attenuates the effects of Msx1. Taken together, our study has uncovered a new function of YB1/p32, a regulator of skeletal muscle differentiation.

  2. Y-box Binding Protein-1 Enhances Oncogenic Transforming Growth Factor β Signaling in Breast Cancer Cells via Triggering Phospho-Activation of Smad2.

    Science.gov (United States)

    Stope, Matthias B; Weiss, Martin; Koensgen, Dominique; Popp, Simone L; Joffroy, Christian; Mustea, Alexander; Buck, Miriam B; Knabbe, Cornelius

    2017-12-01

    Transforming growth factor β (TGFβ) plays a role in diverse oncogenic pathways including cell proliferation and cell motility and is regulated by the pleiotropic factor Y-box binding protein-1 (YB-1). In breast cancer, Sma/Mad related protein 2 (Smad2) represents the most common downstream transducer in TGFβ signaling. Here, YB-1's impact on Smad2 phospho-activation was characterized by incubation of the breast cancer cell line MCF-7 with or without TGFβ1 in the absence or presence of overexpressed YB-1 protein. The phospho-status of Smad2 was assessed via western blotting. Analysis of MCF-7 cells revealed no induction of total Smad2 neither in the presence of TGFβ1, nor during YB-1 overexpression. In contrast, incubation with TGFβ1 led to an increase of phosphorylated Smad2 forms which was significantly amplified by simultaneously overexpressed YB-1 (2.8±0.2-fold). Oncogenic YB-1 indirectly enhances TGFβ signaling cascades via Smad2 phospho-activation and may represent a promising factor for future diagnosis and therapy of breast cancer. Copyright© 2017, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

  3. YB1/p32, a nuclear Y-box binding protein 1, is a novel regulator of myoblast differentiation that interacts with Msx1 homeoprotein

    International Nuclear Information System (INIS)

    Song, Young Joon; Lee, Hansol

    2010-01-01

    Precisely controlled cellular differentiation is essential for the proper development of vertebrate embryo and deregulated differentiation is a major cause of many human congenital diseases as well as cancer. Msx1 is a member of the homeoprotein family implicated in these processes, which inhibits the differentiation of skeletal muscle and other cell types, presumably by regulating transcription of target genes through interaction with other cellular factors. We presently show that YB1/p32, a nuclear Y-box binding protein 1, interacts with Msx1 homeoprotein and functions as a regulator of C2C12 myoblast differentiation. We demonstrate that YB1/p32 functionally interacts with Msx1 through its N-terminal region and colocalizes with Msx1 at the nuclear periphery. Moreover, we find that YB1/p32 is competent for inhibition of C2C12 myoblast differentiation, which is correlated with its activity as a negative regulator of MyoD gene expression and binding to the MyoD core enhancer region (CER). Furthermore, YB1/p32 cooperates with Msx1 in transcriptional repression and knocking down the expression of endogenous YB1 attenuates the effects of Msx1. Taken together, our study has uncovered a new function of YB1/p32, a regulator of skeletal muscle differentiation.

  4. Induction of fibroblast growth factor 21 does not require activation of the hepatic X-box binding protein 1 in mice

    Directory of Open Access Journals (Sweden)

    Shantel Olivares

    2017-12-01

    Full Text Available Objective: Fibroblast growth factor 21 (FGF21, a key regulator of the metabolic response to fasting, is highly induced by endoplasmic reticulum (ER stress. The X-box binding protein 1 (Xbp1 is one of several ER stress proteins that has been shown to directly activate the FGF21 promoter. We aimed to determine whether hepatic Xbp1 is required for induction of hepatic FGF21 in vivo. Methods: Mice bearing a hepatocyte-specific deletion of Xbp1 (Xbp1LKO were subjected to fasting, pharmacologic ER stress, or a ketogenic diet, all potent stimuli of Fgf21 expression. Results: Hepatocyte-specific Xbp1 knockout mice demonstrated normal induction of FGF21 in response to fasting or pharmacologic ER stress and enhanced induction of FGF21 in response to a ketogenic diet. Consistent with preserved induction of FGF21, Xbp1LKO mice exhibited normal induction of FGF21 target genes and normal ketogenesis in response to fasting or a ketogenic diet. Conclusion: Hepatic Xbp1 is not required for induction of FGF21 under physiologic or pathophysiologic conditions in vivo. Keywords: Unfolded protein response, Endoplasmic reticulum stress, Fasting, Fatty acid oxidation, Ketogenic diet

  5. Y-box-binding protein 1 interacts with hepatitis C virus NS3/4A and influences the equilibrium between viral RNA replication and infectious particle production.

    Science.gov (United States)

    Chatel-Chaix, Laurent; Melançon, Pierre; Racine, Marie-Ève; Baril, Martin; Lamarre, Daniel

    2011-11-01

    The hepatitis C virus (HCV) NS3/4A protein has several essential roles in the virus life cycle, most probably through dynamic interactions with host factors. To discover cellular cofactors that are co-opted by HCV for its replication, we elucidated the NS3/4A interactome using mass spectrometry and identified Y-box-binding protein 1 (YB-1) as an interacting partner of NS3/4A protein and HCV genomic RNA. Importantly, silencing YB-1 expression decreased viral RNA replication and severely impaired the propagation of the infectious HCV molecular clone JFH-1. Immunofluorescence studies further revealed a drastic HCV-dependent redistribution of YB-1 to the surface of the lipid droplets, an important organelle for HCV assembly. Core and NS3 protein-dependent polyprotein maturation were shown to be required for YB-1 relocalization. Unexpectedly, YB-1 knockdown cells showed the increased production of viral infectious particles while HCV RNA replication was impaired. Our data support that HCV hijacks YB-1-containing ribonucleoparticles and that YB-1-NS3/4A-HCV RNA complexes regulate the equilibrium between HCV RNA replication and viral particle production.

  6. Tata Kelola Publik Dan Kinerja Keuangan Pemerintah Daerah Di Indonesia

    Directory of Open Access Journals (Sweden)

    Handoko A Hasthoro

    2016-08-01

      Tujuan penelitian ini adalah untuk menguji pengaruh penerapan tata kelola publik terhadap kinerja keuangan pemerintah daerah di Indonesia. Pelaksanaan pemerintahan umum diukur dengan menggunakan transparansi pemerintah daerah, akuntabilitas pemerintah daerah, budaya hukum, dan partisipasi masyarakat. Sejumlah lima puluh pemerintah daerah yang disurvei oleh Tansparency International Indonesia pada tahun 2010 digunakan sebagai sampel. Hasil dari analisis regresi berganda menunjukkan bahwa budaya hukum yang ditunjukkan oleh indeks persepsi korupsi, dan partisipasi masyarakat yang diwakili oleh jumlah pemilih pemilu memiliki pengaruh signifikan positif pada kinerja keuangan pemerintah daerah di Indonesia. Hasil penelitian menunjukkan bahwa penegakan hukum dan pemilu yang adil mendorong kepercayaan masyarakat untuk membayar pajak yang meningkatkan pendapatan asli.

  7. VISUALISASI SISTEM TATA SURYA MENGGUNAKAN ANIMASI 3D

    Directory of Open Access Journals (Sweden)

    Achmad Nur Hamid

    2008-02-01

    Full Text Available Multimedia berkembang pesat seiring dengan perkembangan teknologi. Salah satu aspek yang dipengaruhi adalah dunia pendidikan. Dalam dunia pendidikan multimedia digunakan sebagai media pembelajaran. Dengan aplikasi pembelajaran berbasis multimedia, materi yang disajikan diharapkan lebih menarik dan mudah dipahami. Tujuan dari penulisan skripsi ini adalah untuk membuat aplikasi system tatasurya menggunakan animasi 3 dimensi untuk menunjang sarana belajar yang sudah ada seperti buku. Pengumpulan data dalam pembuatan aplikasi ini dilakukan dengan cara mendayagunakan sumber informasi yang terdapat pada perpustakaan dan sumber informasi lain seperti internet. Aplikasi ini dikembangkan dengan metode pengembangan multimedia menurut Luther yaitu concept, design, material collecting, assembly, testing dan distribution. Dalam pembuatan aplikasi ini menggunakan 3DS Max 2009 sebagai software untuk membuat objek-objek yang terdapat pada system tata surya dengan bentuk 3 dimensi, Adobe Premiere Pro sebagai software untuk menggabungkan suara (audio dan video hasil renderan dari 3DS Max, Adobe Flash CS4 Professional di gunakan sebagai penggabungan semua objek multimedia yang telah dibuat oleh perangkat lunak lainnya. Dengan aplikasi ini siswa dapat memahami materi tentang sistem tata surya dan membuat suasana belajar lebih menyenangkan.

  8. X-box binding protein 1 is essential for the anti-oxidant defense and cell survival in the retinal pigment epithelium.

    Directory of Open Access Journals (Sweden)

    Yimin Zhong

    Full Text Available Damage to the retinal pigment epithelium (RPE is an early event in the pathogenesis of age-related macular degeneration (AMD. X-box binding protein 1 (XBP1 is a key transcription factor that regulates endoplasmic reticulum (ER homeostasis and cell survival. This study aimed to delineate the role of endogenous XBP1 in the RPE. Our results show that in a rat model of light-induced retinal degeneration, XBP1 activation was suppressed in the RPE/choroid complex, accompanied by decreased anti-oxidant genes and increased oxidative stress. Knockdown of XBP1 by siRNA resulted in reduced expression of SOD1, SOD2, catalase, and glutathione synthase and sensitized RPE cells to oxidative damage. Using Cre/LoxP system, we generated a mouse line that lacks XBP1 only in RPE cells. Compared to wildtype littermates, RPE-XBP1 KO mice expressed less SOD1, SOD2, and catalase in the RPE, and had increased oxidative stress. At age 3 months and older, these mice exhibited apoptosis of RPE cells, decreased number of cone photoreceptors, shortened photoreceptor outer segment, reduced ONL thickness, and deficit in retinal function. Electron microscopy showed abnormal ultrastructure, Bruch's membrane thickening, and disrupted basal membrane infolding in XBP1-deficient RPE. These results indicate that XBP1 is an important gene involved in regulation of the anti-oxidant defense in the RPE, and that impaired activation of XBP1 may contribute to RPE dysfunction and cell death during retinal degeneration and AMD.

  9. Biological activity of Penaeus monodon GILT in shrimp pathogen protection

    Directory of Open Access Journals (Sweden)

    Aekkaraj Nualla-ong

    2017-04-01

    Full Text Available Gamma-interferon-inducible lysosomal thiol reductase (GILT contains a CXXC active site motif that possesses thiol reductase activity by catalyzing the disulfide bond reduction of exogenous antigens. Mutating the active site of human GILT to change the cysteine residues to serine residues eliminates this property. Our previous study reported that Penaeus monodon GILT (PmGILT contained a CXXS active site motif. Therefore, we assessed the enzymatic activity of PmGILT and demonstrated that it displayed identical thiol reductase activity at an acidic pH. In addition, the biological activity of PmGILT against shrimp pathogens, including white spot syndrome virus (WSSV and Gram-negative bacteria, was investigated. The neutralization of WSSV with PmGILT indicated the inhibition of WSSV invasion into shrimp hemocyte cells. Moreover, the relative percentage survival of shrimp injected with PmGILT-treated virus solution was 75%. Finally, the antimicrobial activity of PmGILT was confirmed by the growth inhibition of Vibrio harveyi. These results establish the role of PmGILT in the inhibition of the virulence of two major shrimp pathogens.

  10. Effects of decomposing Rhizophora apiculata leaves on larvae of the shrimp Penaeus monodon

    NARCIS (Netherlands)

    Roijackers, R.M.M.; Nghia, T.T.; Ut, V.N.; Scheffer, M.

    2006-01-01

    We studied the effects of different concentrations of decomposing Rhizophora apiculata leaves and their leachates on larvae of the shrimp Penaeus monodon under laboratory conditions. Shrimp mortality was highly dependent on the concentration of oxygen in the water, which in turn was strongly

  11. Protection of Penaeus monodon against white spot syndrome virus using a WSSV subunit vaccine

    NARCIS (Netherlands)

    Witteveldt, J.; Vlak, J.M.; Hulten, van M.C.W.

    2004-01-01

    Although invertebrates lack a true adaptive immune response, the potential to vaccinate Penaeus monodon shrimp against white spot syndrome virus (WSSV) using the WSSV envelope proteins VP19 and VP28 was evaluated. Both structural WSSV proteins were N-terminally fused to the maltose binding protein

  12. Elimination of Chloramphenicol by Tiger Shrimp (Penaeus monodon and White Shrimp (Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    Heny Suseno

    2016-07-01

    Full Text Available Chloramphenicol (CAP has been illegally used in many shrimp farms in South East Asia, including Indonesia. We performed an experiment of elimination simulation of CAP in tiger shrimp (Penaeus monodon and white shrimp (Litopenaeus vannamei. After 5 days of depuration process, the concentration of CAP in P. monodon decreased to 94.85% (muscle, 97.98% (cephalothoraxes, and 90.30% (exoskeleton. The elimination half-life of CAP in P monodon was 0.596 day in the muscle, 0.716 day in cephalothorax, and 0.437 day in exoskeleton. On the other hand, concentrations of CAP in L. vannamei decreased to 97.74% (muscle, 90.30% (cephalothoraxes, and 97.63% (exoskeleton. The elimination half-life of CAP in L. vannamei was 0.6624 day (muscle, 0.859 day (cephalothorax, and 0.796 day (exoskeleton. CAP was retained better by P. monodon compared to L. vannamei.

  13. Conducta de forrajeo del gastrópodo Acanthina monodon Pallas, 1774 (Gastropoda: Muricidae en el intermareal rocoso de Chile central Foraging behavior of the gastropod Acanthina monodon Pallas, 1774 (Gastropoda: Muricidae in the intertidal rocky shores of central Chile

    Directory of Open Access Journals (Sweden)

    RUBÉN E. SOTO

    2004-03-01

    Full Text Available En el presente trabajo investigamos aspectos de la ecología y conducta de forrajeo de Acanthina monodon, un gastrópodo murícido que habita en el intermareal rocoso de Chile central. En terreno, estudiamos las variaciones temporales en su distribución, densidad y dieta. En el laboratorio, cuantificamos la tasa de consumo, las preferencias alimentarias, el tiempo de ingestión y la rentabilidad energética obtenida con distintos tipos de presas mediante experimentos y registros en video. Las mayores densidades de individuos de A. monodon fueron observadas en la franja intermareal cercana al nivel cero de marea. En terreno, A. monodon realiza sus actividades de forrajeo principalmente durante la noche y su dieta consistió principalmente de mitílidos (95 % y cirripedios (5 %. La composición de la dieta de A. monodon en terreno presentó variaciones temporales las cuales dependerían principalmente de cambios en la oferta de los distintos tipos de mitílidos presentes en terreno durante los dos años de muestreo. En el laboratorio, los individuos de Acanthina presentaron preferencias alimentarias significativas por el mitílido Semimytilus algosus. En general, A. monodon bajo condiciones de laboratorio presentó una conducta de forrajeo en la cual maximizó la ganancia neta de energía, mediante la selección de las especies y tamaños de presas que le retribuyen la mayor rentabilidad energéticaWe investigated the ecology and foraging behavior of Acanthina monodon, a muricid gastropod that inhabits in the intertidal rocky shores of central Chile. In the field, we studied temporal variation of their spatial distribution, density, and diet composition. While in the laboratory, we quantified the consumption rate, alimentary preferences, ingestion times and energy profitability obtained with different types of prey using experiments and video recording. High densities of A. monodon individuals were observed in the intertidal fringe near at the

  14. MiRNA-205 modulates cellular invasion and migration via regulating zinc finger E-box binding homeobox 2 expression in esophageal squamous cell carcinoma cells

    Directory of Open Access Journals (Sweden)

    Yamashita Shunichi

    2011-03-01

    Full Text Available Abstract Background Esophageal squamous cell carcinoma (ESCC is often diagnosed at later stages until they are incurable. MicroRNA (miR is a small, non-coding RNA that negatively regulates gene expression mainly via translational repression. Accumulating evidence indicates that deregulation of miR is associated with human malignancies including ESCC. The aim of this study was to identify miR that could be specifically expressed and exert distinct biological actions in ESCC. Methods Total RNA was extracted from ESCC cell lines, OE21 and TE10, and a non-malignant human esophageal squamous cell line, Het-1A, and subjected to microarray analysis. Expression levels of miR that showed significant differences between the 2 ESCC and Het-1A cells based on the comprehensive analysis were analyzed by the quantitative reverse transcriptase (RT-PCR method. Then, functional analyses, including cellular proliferation, apoptosis and Matrigel invasion and the wound healing assay, for the specific miR were conducted. Using ESCC tumor samples and paired surrounding non-cancerous tissue obtained endoscopically, the association with histopathological differentiation was examined with quantitative RT-PCR. Results Based on the miR microarray analysis, there were 14 miRs that showed significant differences (more than 2-fold in expression between the 2 ESCC cells and non-malignant Het-1A. Among the significantly altered miRs, miR-205 expression levels were exclusively higher in 5 ESCC cell lines examined than any other types of malignant cell lines and Het-1A. Thus, miR-205 could be a specific miR in ESCC. Modulation of miR-205 expression by transfection with its precursor or anti-miR-205 inhibitor did not affect ESCC cell proliferation and apoptosis, but miR-205 was found to be involved in cell invasion and migration. Western blot revealed that knockdown of miR-205 expression in ESCC cells substantially enhanced expression of zinc finger E-box binding homeobox 2

  15. Nucleonic gauging: a tool for process and quality control at Tata Steel

    International Nuclear Information System (INIS)

    Pandey, J.C.; Manish Raj; Panda, B.N.

    1998-01-01

    In recent years, there has been much progress in utilizing nuclear sources for process and quality control in Integrated Steel Plants. Tata Steel has also taken interest in implementing these gauging techniques and at present 92 such gauges are in use for measuring level, thickness, density, moisture and ash content at various locations of Tata Steel. These gauges function on line and non contact with the material to be inspected and are designed to withstand the hostile Steel Plant Environment. The paper highlights use of different gauges at Tata Steel. (author)

  16. STUDY ON FRY PERFORMANCE OF BLACK TIGER SHRIMP Penaeus monodon WITH SPECIAL REFERENCE TO ITS MORPHOLOGY AND RNA/DNA RATIO ANALYSIS

    OpenAIRE

    Haryanti Haryanti; Ketut Mahardika; Sari Budi Moria; I Gusti Ngurah Permana

    2006-01-01

    Standard method to asses the performance of black tiger shrimp (Penaeus monodon) fry was needed for successful shrimp culture. The main purpose of this study was to determine standard method fry performance of P. monodon assesment based on its morphology and molecular RNA/DNA ratio analysis. Samples of P. monodon fry were collected from hatcheries in Bali, six hatcheries in East Java, three hatcheries in Central Java and six hatcheries in South Sulawesi. Each hatchery gave 25 appropriate size...

  17. Interpreting SCAMPI for a People CMM Appraisal at Tata Consultancy Services

    National Research Council Canada - National Science Library

    Radice, Ron; Hefley, William E; Curtis, Bill; Ferguson, Jack; Hayes, Will

    2005-01-01

    Tata Consultancy Services (TCS) is a large information technology consulting, services, and business process outsourcing organization interested in reducing the costs of conducting process improvement appraisals at its multiple locations...

  18. Penerapan Framework COBIT untuk Identifikasi Tingkat Kematangan Tata Kelola Teknologi Informasi: Studi Kasus di Fasilkom Unwidha

    Directory of Open Access Journals (Sweden)

    Agustinus Suradi

    2017-07-01

    Full Text Available Perkembangan IT sebagai media komunikasi data hingga saat ini berkembang sangat pesat. Peranan teknologi informasi dalam suatu lembaga sangat krusial, namun  masalah  yang sering terjadi di lembaga/ instansi adalah penggunaan teknologi informasi yang ternyata tidak sesuai dengan harapan, oleh karena itu diperlukan tata kelola terhadap penggunaan teknologi informasi. Permasalahan yang dihadapi di Fasilkom Unwidha adalah adanya ketidak puasan layanan IT yang diberikan, terdapatnya gangguan layanan yang tidak segera diatasi, adanya koordinasi maintenance infrastruktur IT yang kurang optimal. Sehingga perlu adanya evaluasi terhadap tata kelola teknologi informasi yang diberikan untuk penyelarasan dengan tujuan organisasi agar visi yang diharapkan dapat tercapai. Penelitian ini membahas tentang kondisi tata kelola teknologi informasi di Fasilkom Unwidha, dan sejauh mana telah menerapkan tata kelola teknologi informasi yang baik. Metode analisis yang digunakan dalam penelitian ini adalah dengan menggunakan  framework Control Objectives for Information and Related Technology (COBIT. Hasil dari analisis ini berupa tingkat kematangan tata kelola  teknologi informasi  yang  mencerminkan  kondisi  tata  kelola  teknologi informasi  di  Fakultas Ilmu Komputer Unwidha. Tingkat kematangan dengan mengacu pada maturity level yang disediakan kerangka kerja COBIT, diperoleh nilai  kematangan 2,84 yaitu pada level Defined Process. Sistem memiliki ruang lingkup Business Goals sebanyak 13, IT Goals sebanyak 18, IT Process sebanyak 30 proses dan 4 Domain.

  19. Annual report 1988-89: Tata Institute of Fundamental Research

    International Nuclear Information System (INIS)

    Isloor, J.D.

    1989-01-01

    The annual report surveys the work of the Tata Institute of Fundamental Research (TIFR), Bombay, during the fiscal year 1988-89. Most of the research activities are organised and carried out in two schools of the Institute, namely, the School of Mathematics and the School of Physics. In the School of Mathematics, active research is carried out in almost every branch of pure mathematics. The School of Physics is engaged in research activities of both theoretical and experimental nature in high energy physics, astrophysics, cosmic rays, space physics, astronomy, nuclear and atomic physics, condensed matter physics, molecular biology, computer science and communication and microwave engineering. TIFR has a Basic Dental Research Unit which carries out intervention studies on oral cancer and precancerous lesions. Its Homi Bhabha Centre for Science Education (HBCSE) carries out programmes for improvement of science education at all levels in the country. TIFR outstation units are: (1) TIFR Centre at Bangalore which in collaboration with the Indian Institute of Science, Bangalore has a programme in Applications of Mathematics (2) Radio Astronomy Centre, Ooty, (3) Baloon Facility, Hyderabad and (4) National Image Processing Facility, Ooty. The academic and research activities of the schools, new technique and instruments and experimental facilities developed and fabricated by various units of TIFR are briefly described. Faculty and section wise list of staff members is given. Lists of publications by the members and other activities such as colloquia, seminars, conferences etc. are also given. (M.G.B.)

  20. Tata Institute of Fundamental Research School of Physics

    International Nuclear Information System (INIS)

    Daniel, R.R.

    1975-01-01

    The diverse activities currently in progress in the School of Physics at Tata Institute of Fundamental Research, Bombay are reported in detail. The activities involving theoretical and experimental research are grouped under the following areas: (1) pure physics (2) astronomy and space science (3) chemical and biological studies and (4) applied research. In pure physics, studies are in progress in nuclear physics, high energy physics and solid state physics. In astronomy and space science, the fields of investigation comprise: cosmic ray physics, theoretical astrophysics and radio-astronomy. In chemical physics, structure of a variety of systems have been investigated using NMR and Moessbauer techniques. In molecular biology, basic biological processes have been studied in terms of structure and properties of biomolecules. In addition to these areas of pure research, considerable advances have been made in computer science and technology, solid state electronics, microwave engineering and hydrogy. The work done in each one of these areas is briefly summarized. A number of supporting research facilities are mentioned. A brief mention has also been made on the existing education and training programmes. (A.K.)

  1. SISTEM Tata niaga KEDELAI DI DESA CIPEUYEUM, KECAMATAN HAURWANGI, KABUPATEN CIANJUR

    Directory of Open Access Journals (Sweden)

    Aldha Hermianty Alang

    2014-09-01

    Full Text Available ABSTRACTThe objectives of this research were 1 identify the channel, institution, and trade system function of soy in Cipeuyeum Village, Haurwangi District, Cianjur, 2 Analyze the structure and the behavior of soy market in Cipeuyeum Village, Haurwangi District, Cianjur, also 3 Analyze the efficiency of soy trade system at each channel with trade channel margin approach, farmers’ share and profit-cost ratio (π/c. Qualitative and quantitative analysis were used to analyze the data. Qualitative analysis includes: channel and institution of the trade system, analysis of farmers and institutions functions involved in the trade system, analysis of market structure and behavior, trade system margin analysis, farmers’ share analysis, and analysis of π/c ratio.  There are four channels of marketing system in this village with five institutions that identified with the snowball method. The institutions within the trade systems perform their own function and face diverse market structures. Quantitative analysis showed that the most efficient channel is the one with Rp917 for the trade margin, 85,89% for the farmer’s share value, and 7,06 for profit-cost ratio. Therefore, Soybean Farmers should not sell the entire stock system directly to large traders without going through the small traders as it will increase the share of the farmers.Keywords: soy agribusiness, Cipeuyeum village, demand for soy, trade system of soy, soyABSTRAKTujuan dari penelitian ini adalah 1 mengidentifikasi saluran, lembaga, dan fungsi tata niaga kedelai di Desa Cipeuyeum, Kecamatan Haurwangi, Kabupaten Cianjur, 2 menganalisis struktur dan perilaku pasar kedelai di Desa Cipeuyeum, Kecamatan Haurwangi, Kabupaten Cianjur, dan 3 menganlisis efisiensi tata niaga kedelai pada setiap saluran yang ada dengan pendekatan margin tata niaga, farmer’s share, serta rasio keuntungan terhadap biaya (π/c. Analisis data dilakukan secara kualitatif dan kuantitatif. Analisis kualitatif

  2. BIOACCUMULATION AND DEPURATION STUDIES OF LEAD NITRATE ON ECONOMICALLY IMPORTANT SHRIMP PENAEOUS MONODON

    OpenAIRE

    S.Palani Kumar; A.S.Sharadhamma

    2013-01-01

    Current study of Bioaccumulation and Depuration of Lead nitrate on marine shrimp Penaeous monodon shows Lead nitrate accumulation more in hepatopancreas compared to muscle and the depuration level of heavy metal Lead nitrate was more through hepatopancreas comparing to muscle tissues, when comparing to the control animals.This shows the major role of Hepatopacreas in Bioaccumulation and depuration in this study on muscle tissues and hepatopancreas tissues

  3. Effects of extraction parameters on physicochemical and functional characteristics of chitosan from Penaeus monodon shell

    Directory of Open Access Journals (Sweden)

    Jubril Olayinka Akolade

    2016-11-01

    Full Text Available Objective: To investigate the effect of extraction parameters with particular interest during the microwave deacetylation process on the characteristics of chitosan produced from Penaeus monodon (P. monodon sourced within the coastal region of Lagos, Nigeria for applications of controlled release systems for pharmaceutical industries. Methods: Chitosan was extracted from shrimp (P. monodon shell and evaluated as a controlled release system for curcumin. Effects of relevant processing parameters on physicochemical and functional characteristics of the extracted chitosan were assessed. The crude chitosan was purified and used to prepare controlled release formulations for curcumin via ionic gelation with tripolyphosphate. Results: Data from the study showed that increasing time and temperature during deproteinization significantly improved the removal of protein bound to the shell matrix. Also, the ratio of the weight of the deproteinized sample to the volume of HCl used for demineralization influenced the process. During microwave-assisted production of chitosan from chitin, increase in the concentration of the deacetylating medium significantly increased solubility, viscosity and degree of deacetylation, whereas increasing temperature and time during deacetylation of chitin degraded the biopolymer to give low molecular weight chitosan. Optimized extraction and purification process yielded absolutely soluble medium to low molecular weight chitosan. The encapsulation efficiency, loading capacity, percentage yield, release efficiencies in simulated gastric and intestinal fluids of curcumin loaded in the formulations of chitosan from P. monodon were compared favorably to encapsulation and release characteristics of the encapsulated curcumin in commercially available chitosan used as the reference. Conclusions: Valorization of shrimp waste into pharmaceutically graded medium molecular weight chitosan was achieved. The chitosan obtained can be used as

  4. Transferable chloramphenicol resistance determinant in luminous Vibrio harveyi from penaeid shrimp Penaeus monodon larvae

    Directory of Open Access Journals (Sweden)

    Thangapalam Jawahar Abraham

    2016-12-01

    Full Text Available Antibiotic-resistant luminous Vibrio harveyi strains isolated from Penaeus monodon larvae were screened for the possession of transferable resistance determinants. All the strains were resistant to chloramphenicol and the determinant coding for chloramphenicol resistance was transferred to Escherichia coli at frequencies of 9.50x10-4 to 4.20x10-4. The results probably suggest the excessive use of chloramphenicol in shrimp hatcheries to combat luminous vibriosis.

  5. GENETIC DIVERSITY OF Vibrio harveyi ISOLATED FROM TIGER PRAWN Penaeus monodon HATCHERIES AND GROW OUT PONDS

    OpenAIRE

    Muliani Muliani; Ince Ayu Khairana Kadriah; Andi Parenrengi; Sulaeman Sulaeman

    2006-01-01

    ibrio harveyi is known as one among the most harmful bacteria infecting tiger prawn at every stage of its life’s. The present research was aimed to reveal the genetic diversity of Vibrio harveyi isolated from tiger prawn (Penaeus monodon) culture. The samples of bacteria were collected from hatchery (brood-stock, larvae, natural feed, artemia, and larval rearing water) and grow-out (juveniles, water, shrimp, sediment, plankton, crab, mollusc, microalgae, and wild fish).  The taxonomic identif...

  6. Effect of Probiotics on the Hatchery Seed Production of Black Tiger Shrimp, Penaeus monodon (Fabricius)

    OpenAIRE

    P. Soundarapandian; R. Babu

    2010-01-01

    In recent years, the diseases of shrimps hindered the development of shrimp culture. Hence, the use of probiotic bacteria in aquaculture has tremendous scope and the study of the application of probiotics in aquaculture has a glorious future. In the present study, the probiotics was applied (experimental) for the larval rearing of P. monodon which is compared with control tanks (without probiotics). The temperature and alkalinity of both control and experimental tanks were more of less same. ...

  7. STUDY OF PROBIOTICS ON THE SEED PRODUCTION OF BLACK TIGER SHRIMP Penaeus monodon

    OpenAIRE

    M. Abdul Kader; Sheikh Aftab Uddin; M. Nurul Azim Sikder; M. Abdul Hakim; M. Mahbub Alam; Ashraful Haque Azad; Chowdhury Kamrul Hasan

    2013-01-01

    Currently, antibiotics are widely used in shrimp hatcheries to control bacterial infections. Appearance of antibiotic resistant pathogens and restriction on the use of antibiotics have led to the development of alternatives to antibiotics in hatchery systems. In light of this, an attempt was undertaken to investigate the effects of probiotics on the larval rearing of Penaeus monodon, compared with control tanks (without probiotics). The results showed that several issues significantly improve...

  8. Immunomodulatory and Growth-Promoting Potential of Lowcost Probiotic Product in Penaeus Monodon Culture System

    Directory of Open Access Journals (Sweden)

    Chandran Manohar Navin

    2017-06-01

    Full Text Available The capability of a low-cost probiotic product was evaluated in Penaeus monodon reared under an outdoor culture system for a period of 90 days. The low-cost probiotic product was formulated by mixing the broth culture of Bacillus cereus along with commercial chalk powder in 1:1 ratio. The prepared probiotic product as a powdered supplement was incorporated along with the shrimp diet at various concentrations (1 to 5%. The viability of B. cereus in the experimental diet was tested once in 30 days up to 90 days during the experimentation. After 90 days of feeding experiments, the growth performance of shrimp was determined with a maximum production of 11.98 ± 0.367 g, SGR of 5.030 ± 0.171% and FCE of 86.63 ± 1.300% in P. monodon fed D4 diet (4% probiotic supplement. The immunological parameters determined at the end of the culture experiment showed variation among diets. The total haemocyte count (273.66 ± 3.09 x 105 cells/ml, phenoloxidase activity (0.132 ± 0.007 OD and respiratory burst activity (0.291 ± 0.020 O.D were high in P. monodon fed D4 diet. Similar effects were observed for plasma protein concentration (68.00 ± 1.41 mg/ ml, lysozyme activity (0.483 ± 0.014 U/ml and bactericidal activity (81.0 ± 1.33% in shrimp fed D4 diet. The results of the present investigation conclude that the probiotic product at a concentration of 4% was effective in elevating the growth and immune performances in shrimp P. monodon.

  9. Analisis Tata Kelola Teknologi Informasi Menggunakan Kerangka Kerja COBIT 4.1 pada Fakultas Teknik Undip

    Directory of Open Access Journals (Sweden)

    Arini Arumana

    2014-06-01

    Full Text Available Peran teknologi informasi dalam suatu instansi sudah tidak dapat dielakkan lagi, mengingat perkembangannya yang begitu pesat seiring berjalannya waktu. Namun masalah yang sering terjadi di instansi adalah penggunaan teknologi informasi yang kadang tidak sesuai dengan harapan. Oleh karena itu diperlukan tata kelola terhadap penggunaan teknologi informasi yang biasa disebut dengan IT Governance. Fakultas teknik UNDIP adalah salah satu fakultas yang dalam operasionalnya sangat mengandalkan teknologi informasi, baik dalam aktifitas belajar mengajar maupun dalam menjalankan operasional bisnis. Kondisi sekarang di Fakultas Teknik sendiri tidak terdapat suatu indikator yang dapat menyatakan bahwa kinerja TI yang berjalan telah sesuai dengan visi dan misi Fakultas Teknik, oleh karena itu perlu dilakukan analisis tata kelola teknologi informasi, guna mengetahui performa TI sekarang sesuai dengan yang diharapkan oleh managemen atau tidak. Hasil dari analisis ini berupa tingkat kematangan tata kelola TI yang mecerminkan kondisi tata kelola TI di Fakultas Teknik dengan mengacu pada maturity level yang disediakan kerangka kerja COBIT 4.1, yakni dari level 0 (non-existent sampai 5 (optimized. Berdasarkan analisis yang dilakukan, secara garis besar kondisi kematangan tata kelola TI Fakultas Teknik berada pada level 2 yakni repeatable but intuitive. Kondisi ini mengacu pada beberapa kelemahan dalam proses-proses TI yang berjalan, diantaranya penetapan dan dokumentasi tindakan, kebijakan dan prosedur yang minim, serta tidak tersedianya service level yang disetujui bersama.

  10. Invasion of Asian tiger shrimp, Penaeus monodon Fabricius, 1798, in the western north Atlantic and Gulf of Mexico

    Science.gov (United States)

    Fuller, Pam L.; Knott, David M.; Kingsley-Smith, Peter R.; Morris, James A.; Buckel, Christine A.; Hunter, Margaret E.; Hartman, Leslie D.

    2014-01-01

    After going unreported in the northwestern Atlantic Ocean for 18 years (1988 to 2006), the Asian tiger shrimp, Penaeus monodon, has recently reappeared in the South Atlantic Bight and, for the first time ever, in the Gulf of Mexico. Potential vectors and sources of this recent invader include: 1) discharged ballast water from its native range in Asia or other areas where it has become established; 2) transport of larvae from established non-native populations in the Caribbean or South America via ocean currents; or 3) escape and subsequent migration from active aquaculture facilities in the western Atlantic. This paper documents recent collections of P. monodon from the South Atlantic Bight and the Gulf of Mexico, reporting demographic and preliminary phylogenetic information for specimens collected between North Carolina and Texas from 2006 through 2012. The increased number of reports in 2011 and 2012, ranging from 102 mm to 298 mm total length, indicates that an adult population is present in densities sufficient for breeding, which is indicative of incipient establishment. Based on these reports of P. monodon, its successful invasion elsewhere, and its life history, we believe that this species will become common in the South Atlantic Bight and Gulf of Mexico in less than 10 years. Penaeus monodon is an aggressive predator in its native range and, if established, may prey on native shrimps, crabs, and bivalves. The impacts of an established P. monodon population are potentially widespread (e.g., alterations in local commercial fisheries, direct and indirect pressures on native shrimp, crab and bivalve populations, and subsequent impacts on the populations of other predators of those organisms) and should be considered by resource managers. The impacts of P. monodon on native fauna and the source(s) or vector(s) of the invasion, however, remain unknown at this time.

  11. Effective Governance in the Era of Disruptive Changes in Business Illustrated by the Tata Capital Framework

    DEFF Research Database (Denmark)

    Rose, Caspar; Sherman, David; Haraszuk, Anni

    2015-01-01

    Identifying, assessing, managing, and containing risks is a fundamental responsibility of business and ‘business as usual’ in 2015. The question is: How can businesses be effectively governed in an era of disruptive changes while managing risks of business? This article explores – through the case...... example of Tata Capital, part of the Indian, global conglomerate Tata Group – how to effectively govern in an environment of disruptive forces impacting business while managing risk seen from an audit committee and internal audit perspective. Effective governance through regular evaluation of internal...

  12. Tata Kelola Sistem Informasi pada Perusahaan Pelayaran Lepas Pantai

    Directory of Open Access Journals (Sweden)

    Bimo Andono

    2017-05-01

    peningkatan tingkat kematangan Sistem Informasi (SI PT BRL menggunakan kerangka kerja COBIT. Dari hasil penelitian ini dapat diketahui arah yang diinginkan manajemen PT BRL serta dapat disusun beberapa rekomendasi untuk dapat mewujudkannya. Tata kelola TI adalah konsep penting yang berkembang di perusahaan TI dan bertindak sebagai patokan untuk mengukur kematangan organisasi TI (Ayat et al. 2011. Secara keseluruhan proses perencanaan dan implementasi Sistem Informasi (SI di PT BRL sudah mengikuti kaidah-kaidah sesuai dengan kerangka kerja COBIT dan Maturity Level-nya berada pada level: 3 – Defined dimana prosedur kerja telah distandarisasi dan didokumentasikan serta dikomunikasikan melalui proses pelatihan. Telah dimandatkan pula bahwa prosedur ini wajib dipatuhi, namun bagaimanapun juga masih ada deviasi prosedur dalam pelaksanaannya yang belum diakomodasi. Sudah ada prosedur yang memiliki standar dan didokumentasikan dengan baik, sudah ada pelatihan formal untuk mengkomunikasikan prosedur dan kebijakan yang dibuat. Namun pada tahap implementasinya masih tergantung pada individu apakah mau melakukan prosedur yang ditetapkan atau tidak. Prosedur yang dibuat masih terbatas pada bentuk formalisasi dari praktik yang ada.Kata kunci: Audit, COBIT, CSF, KPI, KGI

  13. Characterization of intestinal bacteria in wild and domesticated adult black tiger shrimp (Penaeus monodon.

    Directory of Open Access Journals (Sweden)

    Wanilada Rungrassamee

    Full Text Available The black tiger shrimp (Penaeus monodon is a marine crustacean of economic importance in the world market. To ensure sustainability of the shrimp industry, production capacity and disease outbreak prevention must be improved. Understanding healthy microbial balance inside the shrimp intestine can provide an initial step toward better farming practice and probiotic applications. In this study, we employed a barcode pyrosequencing analysis of V3-4 regions of 16S rRNA genes to examine intestinal bacteria communities in wild-caught and domesticated P. monodon broodstock. Shrimp faeces were removed from intestines prior to further analysis in attempt to identify mucosal bacterial population. Five phyla, Actinobacteria, Fusobacteria, Proteobacteria, Firmicutes and Bacteroidetes, were found in all shrimp from both wild and domesticated environments. The operational taxonomic unit (OTU was assigned at 97% sequence identity, and our pyrosequencing results identified 18 OTUs commonly found in both groups. Sequences of the shared OTUs were similar to bacteria in three phyla, namely i Proteobacteria (Vibrio, Photobacterium, Novosphingobium, Pseudomonas, Sphingomonas and Undibacterium, ii Firmicutes (Fusibacter, and iii Bacteroidetes (Cloacibacterium. The shared bacterial members in P. monodon from two different habitats provide evidence that the internal environments within the host shrimp also exerts selective pressure on bacterial members. Intestinal bacterial profiles were compared using denaturing gradient gel electrophoresis (DGGE. The sequences from DGGE bands were similar to those of Vibrio and Photobacterium in all shrimp, consistent with pyrosequencing results. This work provides the first comprehensive report on bacterial populations in the intestine of adult black tiger shrimp and reveals some similar bacterial members between the intestine of wild-caught and domesticated shrimp.

  14. RESPON UDANG WINDU ( Penaeus monodon Fabr. TERHADAP ANTIGEN WSSV YANG DIINAKTIVASI DENGAN FORMALDEHID

    Directory of Open Access Journals (Sweden)

    Melta Rini Fahmi

    2016-11-01

    Full Text Available Penelitian ini bertujuan untuk mengetahui respon udang windu (Penaeus monodon terhadap pemberian antigen virus WSSV (White Spot Syndrome Virus yang diinaktifkan dengan menggunakan formaldehid. Penelitian dibagi menjadi dua tahapan, tahapan pertama yaitu menentukan nilai VID50 (Virus Infective Doze dengan Rancangan Acak Lengkap, tahap kedua untuk melihat pengaruh pemberian virus WSSV yang diinaktifkan dengan formaldehid terhadap respon imunitas, tingkah laku, dan tingkat sintasan. Pada tahap kedua penelitian dilakukan secara faktorial, faktor yang digunakan adalah konsentrasi virus terdiri atas 2 level serta konsentrasi formaldehid yang terdiri atas 3 level. Masing-masing kombinasi di atas dibuat sebanyak 6 kali, yang digunakan untuk 3 kelompok penelitian yaitu kelompok tanpa diuji tantang, diuji tantang dilakukan setelah 14 hari, dan uji tantang dilakukan setelah 21 hari, dilaksanakan sebanyak 2 kali ulangan. Untuk semua kelompok percobaan respons udang paling sensitif berupa berenang ke permukaan terjadi setelah 1 jam perlakuan diberikan, diikuti oleh penurunan aktivitas dan penurunan nafsu makan. Tingkat kerusakan organ paling tinggi terdapat pada kelompok penelitian ke-2 (uji tantang setelah 14 hari. Untuk kelompok 1 (divaksinasi kondisi organ hampir normal, hal ini menandakan virus berhasil dilemahkan dan mampu memacu timbulnya antibodi. Tingkat sintasan udang lebih tinggi setelah diuji tantang dibandingkan yang tidak divaksinasi. The purpose of the research was to determine response of black tiger shrimp (Penaeus monodon toward formaldehyde inactivated White Spot Syndrome Virus (WSSV.The study was divided into two phases, the first phase was to determine the VID50 (Virus Infective Doze using Completely Randomize Design, the second phase was to determine of effectiveness of inactive WSSV antigen on the immune response of Penaeus monodon, behavior and pathological respond of Penaeus monodon larvae. VID50 value determined during research was

  15. Ultrastructural and pathogenesis of Monodon baculovirus in SPF shrimp, Litopenaeus vannamei imported to Iran

    OpenAIRE

    Bahari-Meimandi, S.A.; Afsharnasab, M.; Motallebi Moghanjoghi, A.A.; Azaritakami, G.; Sharifrohani, M.

    2014-01-01

    Viral pathogens are major causes of outbreaks in shrimp farms throughout the world. Monodon baculovirus has been known to be invasive in 85-100% of the shrimp hatcheries, in early or late stages of shrimp. Three-hundred and sixty juvenile of Litopenaeus vannamei with average (±SD) size of 7.99±0.54 g and 3600 post larvae 10-15 were prepared from Shrimp Research Station located in Helleh and 3 hatcheries from Bushehr Province, southern part of Iran, respectively. They were allocated to 9 glass...

  16. KARAKTERISTIK SEKUEN cDNA PENGKODE GEN ANTI VIRUS DARI UDANG WINDU, Penaeus monodon

    Directory of Open Access Journals (Sweden)

    Andi Parenrengi

    2016-11-01

    Full Text Available Transgenesis pada ikan merupakan sebuah teknik modern yang berpotensi besar dalam menghasilkan organisme yang memiliki karakter lebih baik melalui rekombinan DNA gen target termasuk gen anti virus dalam peningkatan resistensi pada udang. Gen anti virus PmAV (Penaeus monodon Anti Viral gene merupakan salah satu gen pengkode anti virus yang berasal dari spesies krustase. Penelitian ini dilakukan untuk mengetahui karakteristik gen anti virus yang diisolasi dari udang windu, Penaeus monodon. Isolasi gen anti virus menggunakan metode Polymerase Chain Reaction (PCR dan selanjutnya dipurifikasi untuk sekuensing. Data yang dihasilkan dianalisis dengan program Genetyx Versi 7 dan basic local alignment search tool (BLAST. Hasil penelitian menunjukkan bahwa gen anti virus PmAV yang berhasil diisolasi dari cDNA udang windu dengan panjang sekuen 520 bp yang mengkodekan 170 asam amino. BLAST-N menunjukkan tingkat similaritas yang sangat tinggi (100% dengan gen anti virus yang ada di GeneBank. Komposisi asam amino penyusun gen anti virus yang paling besar adalah serin (10,00%, sedangkan yang terkecil adalah asam amino prolin dan lisin masing-masing 1,76%. Analisis sekuen gen dan deduksi asam amino (BLAST-P memperlihatkan adanya C-type lectin-like domain (CTLD yang memiliki kemiripan dengan gen C-type lectin yang diisolasi dari beberapa spesies krustase. Transgenic fish technology is a potential modern technique in producing better character organism through DNA recombinant of target genes including anti viral gene for improvement of shrimp immunity. PmAV (Penaeus monodon Anti Viral gene is one of anti viral genes isolated from crustacean species. The research was conducted to analyze the characteristics anti viral gene isolated from tiger prawn, Penaeus monodon. Anti viral gene was isolated using Polymerase Chain Reaction (PCR technique and then purified for sequencing. Data obtained were analyzed using Genetyx Version 7 software and basic local alignment

  17. Urea as a Nitrogen Source in a Black Tiger Shrimp (Penaeus monodon) Closed Culture System

    OpenAIRE

    Supannee SUWANPAKDEE; Sataporn DIREKBUSARAKOM; Piyapong CHOTIPUNTU; Putth SONGSANGJINDA

    2010-01-01

    Urea [(NH2)2CO] is an organic compound that serves an important role in the metabolism of nitrogen-containing compound by animals. Urea is widely used in aquaculture systems. This study investigated the effects of urea on growth of Penaeus monodon. Shrimp were reared in 500 l fiber tanks. There was no exchange of water throughout the experiment. Shrimp with an average body weight of 10.99 ± 0.19 g were stocked at a density of 32 shrimp/m2 in 20 ppt diluted seawater and fed with 38 % protein d...

  18. Moult-inhibiting fusion protein augments while polyclonal antisera attenuate moult stages and duration in Penaeus monodon

    Digital Repository Service at National Institute of Oceanography (India)

    Vrinda, S.; Jasmin, C.; Sivakumar, K.C.; Jose, B.; Philip, R; BrightSingh, I

    thioredoxin-fused mature MIH I protein (mf-PmMIH I) of P. monodon in a bacterial system and its use as antigen to raise polyclonal antiserum (anti-mf-PmMIH I). The mature MIH I gene of 231 bp, that codes for 77 amino acids, was cloned into the Escherichia coli...

  19. Chemical and physical effects of crowding on growth and survival of Penaeus monodon Fabricius post-larvae

    NARCIS (Netherlands)

    Nga, B.T.; Lürling, M.F.L.L.W.; Peeters, E.T.H.M.; Roijackers, R.M.M.; Scheffer, M.; Nghia, T.T.

    2005-01-01

    The hypothesis that crowding effects through physical and/or chemical interference may be an important factor in lowering the chance of survival and reducing growth of Penaeus monodon post-larvae under high stocking densities was tested. To separate physical interference from chemically-exerted

  20. Occurrence of viral pathogens in Penaeus monodon post-larvae from aquaculture hatcheries

    Directory of Open Access Journals (Sweden)

    Toms C. Joseph

    2015-09-01

    Full Text Available Viral pathogens appear to exert the most significant constraints on the growth and survival of crustaceans under culture conditions. The prevalence of viral pathogens White Spot Syndrome Virus (WSSV, Hepatopancreatic Parvo Virus (HPV, Monodon Baculo Virus (MBV and Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV in Penaeus monodon post-larvae was studied. Samples collected from different hatcheries and also samples submitted by farmers from Kerala were analyzed. Out of 104 samples collected, WSSV was detected in 12.5% of the post-larvae samples. Prevalence of concurrent infections by HPV, MBV and WSSV (either dual or triple infection was present in 60.6% of the total post-larvae tested. Out of the 51 double positives, 98% showed either HPV or IHHNV infection. HPV or IHHNV was detected in 11 post-larval samples showing triple viral infection. This is the first report of IHHNV from India. Result of this study reveals the lack of efficient screening strategies to eradicate viruses in hatchery reared post-larvae.

  1. STUDY OF PROBIOTICS ON THE SEED PRODUCTION OF BLACK TIGER SHRIMP Penaeus monodon

    Directory of Open Access Journals (Sweden)

    M. Abdul Kader

    2013-10-01

    Full Text Available Currently, antibiotics are widely used in shrimp hatcheries to control bacterial infections. Appearance of antibiotic resistant pathogens and restriction on the use of antibiotics have led to the development of alternatives to antibiotics in hatchery systems. In light of this, an attempt was undertaken to investigate the effects of probiotics on the larval rearing of Penaeus monodon, compared with control tanks (without probiotics. The results showed that several issues significantly improved with administering probiotics in the experimental tanks compared with the tanks without probiotics. For example, the concentration of ammonia was estimated to be 1.25 mg/L that was less than half of what was measured in the control tanks. The size variation was observed more in the control tanks than in the experimental tanks. Moreover, the muscle gut ratio of PL15 was about 85 to 92% in the probiotic treated tank and 70 to 80% in the control tank during the eight cycles of production. The fouling organisms were more in the control tank compared to the experimental tanks. The average length of PL15 was maximum when reared in the experimental tanks compared to the control tanks. The final survival rate of PL15 from the control and experimental tank was 35 and 52%, respectively. The present investigation indicated that probiotics played an important role in the growth, survival and health status of P. monodon larvae.

  2. Microbiome analysis and detection of pathogenic bacteria of Penaeus monodon from Jakarta Bay and Bali.

    Science.gov (United States)

    Oetama, Vincensius S P; Hennersdorf, Philipp; Abdul-Aziz, Muslihudeen A; Mrotzek, Grit; Haryanti, Haryanti; Saluz, Hans Peter

    2016-09-30

    Penaeus monodon, the Asian black tiger shrimp is one of the most widely consumed marine crustaceans worldwide. In this study, we examine and compare the fecal microbiota of P. monodon from highly polluted waters around Jakarta Bay, with those of less polluted waters of Bali. Using next generation sequencing techniques, we identified potential bacterial pathogens and common viral diseases of shrimp. Proteobacteria (96.08%) was found to be the most predominant phylum, followed by Bacteriodetes (2.32%), Fusobacteria (0.96%), and Firmicutes (0.53%). On the order level, Vibrionales (66.20%) and Pseudoaltermonadales (24.81%) were detected as predominant taxa. qPCR profiling was used as a confirmatory step and further revealed Vibrio alginolyticus and Photobacterium damselae as two potential pathogenic species present in most of the samples. In addition, viral diseases for shrimp were discovered among the samples, WSSV in Jakarta free-living samples, YHV in Bali free-living samples and IHHNV in both. Copyright © 2016 Elsevier Ltd. All rights reserved.

  3. Efficiency of probiotics (Ecoforce in the growth and survival of Peneaus monodon

    Directory of Open Access Journals (Sweden)

    V. Elumalai

    2013-12-01

    Full Text Available Probiotic supplementation of live microorganisms in aquaculture aids in preventing disease, thereby increasing production and decreasing economic loss. Application of probiotics bacteria in aquaculture systems plays significant role that determines the fate and success rate of culture. The present study was carried out to evaluate the performance of commercially available probiotics (The major active ingredients include Streptococcus faecalis, Streptococcus faecium, Bacillus mesentericus, Bacillus subtilis, Bacillus natto, Clostridium butyricum, Saccharomyces cerevivisiae, Alkaline Protease and Lipase in the growth and survival of Peneaus monodon in grow-out conditions. At the end of the culture, the survival rate of Pond I (treatment pond was 76%, whereas for Pond II (control pond it was only 59%. Pond I (29.2g where as shrimps in the Pond II showed the least average body weight of 22.6g. It is evident that application of probiotics has improved the growth and survival of P. monodon and which in turn paved way to reap better profit for the farmers. This investigation showed that administration of mixed Bacillus probiotics significantly improved growth and survival of shrimp, increased beneficial bacteria in shrimp culture and enhanced water quality for parameter., it seems likely that the use of probiotics will gradually increase in the days to come that will open new vistas in the arena of aquaculture.

  4. PERENCANAAN TATA GUNA LAHAN DAS WAY SEPUTIH HULU LAMPUNG TENGAH MENGGUNAKAN MODEL TATA AIR (Landuse Planning for Way Seputih Watershed at Central Lampung by Water Management Model

    Directory of Open Access Journals (Sweden)

    Mohammad Amin

    2008-11-01

    Full Text Available ABSTRAK Das Way Seputih Hulu merupakan sungai yang terletak di Kabupaten Larnpung Tengah, merniliki luas 175,28 km2. Kejadian banjir di Desa Segalarninder hilir Way Seputih Hulu terjadi setiap datang musim penghujan. Hasil penelitian bempa software program tata-air.exe, konsep dasar rnodel menggunakan neraca air (waterbalance yang dibuat dengart program delphi versi 7. Hasil uji t-tes antara tebal aliran model simulasi dengan pengukuran lapangan menunjukkan nilai t-hitung (0,14 lebih besar dari t-tabel (2,51, sehingga dikatakan model dapat digunakan untuk melakukan simulasi berbagai alternatif penggunaan lahan. Hasil simulasi program tata-air.exe menunjukkan bahwa keseluruhan alternatif untuk eksperimentasi model menghasilkan nilai rasio tebal aliran di bawah angka 30. Kondisi rasio tebal aliran air Way Seputih Hulu memiliki nilai berkisar antara 2,84 sanrpai 3,40, sehingga dapat dikatakan dalam kondisi masih wajar bahkan dapat dikatakan mempunvai nilai yang cukup bagus. Alternatif penggunaan lahan yang mengarah kepada bentuk lahan kebun campuran akan memberikan nilai rasio paling kecil dan menghasilkan produksi air yang kecil pula.   ABSTRACT  Watersheed of Way Seputih Upstream represent river which located in Kabupaten Lampung Tengah, oing wide of 175,28 km2. Occurence of floods in Countryside of Segalaminder go downstream Way Seputih Upstream happened in every coming rain season. Result of the research namely tata-air.exe, base concept of modeling uses water balance was made by using Delphi 7th version. Result of t-test value between modeled discharge flow and field measurement result shows t-counting value (0.14 higher than t-table (2.51, hence the model is can be used for simulating various landuse alternative. The simulation result using tata-air.exe shows that entire alternatives for model experimentation resulted discharge ratio value was under 30. Condition of Way Seputih Hulu River discharge was in the range between 2.84 until 3

  5. Prognostic implications of the nuclear localization of Y-box-binding protein-1 and CXCR4 expression in ovarian cancer: their correlation with activated Akt, LRP/MVP and P-glycoprotein expression.

    Science.gov (United States)

    Oda, Yoshinao; Ohishi, Yoshihiro; Basaki, Yuji; Kobayashi, Hiroaki; Hirakawa, Toshio; Wake, Norio; Ono, Mayumi; Nishio, Kazuto; Kuwano, Michihiko; Tsuneyoshi, Masazumi

    2007-07-01

    The nuclear localization of Y-box-binding protein-1 (YB-1) is known to be a poor prognostic factor in several human malignancies, including ovarian carcinoma. Following on from our basic study dealing with microarray analyses of YB-1-associated gene expression in ovarian cancer cells, we examined whether nuclear localization of YB-1 is associated with the expression of CXCR4, a vault protein named lung resistance-related vault protein (LRP/MVP), phosphorylated Akt (p-Akt) or P-glycoprotein (P-gp) in human ovarian carcinoma. Fifty-three surgically resected ovarian carcinomas treated with paclitaxel and carboplatin were examined immunohistochemically for nuclear YB-1 expression and intrinsic expression of p-Akt, P-gp, LRP/MVP and CXCR4. Nuclear expression of YB-1 demonstrated significant correlation with p-Akt, P-gp and LRP expression, but no relationship with CXCR4 expression. By multivariate analysis, only YB-1 nuclear expression and CXCR4 expression were independent prognostic factors with regard to overall survival. These results indicate that YB-1 nuclear expression and CXCR4 expression are important prognostic factors in ovarian carcinoma.

  6. Molecular characterization and expression pattern of X box-binding protein-1 (XBP1) in common carp (Cyprinus carpio L.): Indications for a role of XBP1 in antibacterial and antiviral immunity.

    Science.gov (United States)

    Li, Ting; Li, Hua; Peng, Shaoqing; Zhang, Fumiao; An, Liguo; Yang, Guiwen

    2017-08-01

    X box-binding protein-1 (XBP1) is a transcription factor that is essential for the unfolded protein response (UPR) and the differentiation of plasma cells, and some findings have also uncovered its function in innate immunity. XBP1 typically has two different transcripts, un-spliced (XBP1u) and spliced forms (XBP1s), but XBP1s is an active transcription factor in the regulation of target genes. To date, there is no evidence about the identification and function of XBP1 in common carp. Moreover, no data are currently available regarding the role of fish XBP1 in innate immunity. Thus, to determine whether XBP1 is involved in innate immune response in common carp, we cloned CcXBP1s and examined the expression of XBP1s and a XBP1s stimulated gene (IL-6) after Aeromonas hydrophila (A. hydrophila) and polyinosinic-polycytidylic acid (polyI:C) challenges. The results imply that CcXBP1s, as an active transcription factor, might play regulation roles in the antibacterial and antiviral innate immune responses of common carp. This allows us to gain new insights into the immunological function of XBP1 in fish innate immunity and the evolution of this important class of genes across vertebrates. Copyright © 2017 Elsevier Ltd. All rights reserved.

  7. USULAN PERANCANGAN TATA LETAK PENYIMPANAN KOMPONEN BERDASARKAN KRITERIA KOMODITI KOMPONEN (Studi Kasus di PT Triangle Motorindo Semarang

    Directory of Open Access Journals (Sweden)

    Sri Hartini

    2012-02-01

    Full Text Available Kurang lancarnya suplai komponen ke bagian produksi dapat menyebabkan menurunnya produktivitas dari bagian produksi. Untuk itu perlu dilakukan perancangan tata letak penyimpanan komponen yang lebih baik sehingga dapat mengurangi waktu mencari komponen, mengurangi jarak perjalanan operator dalam pengambilan dan pengiriman komponen, dan meningkatkan pemanfaatan kapasitas gudang.                 PT. Triangle Motorindo merupakan perusahaan yang bergerak dalam usaha perakitan sepeda motor. Secara garis besar, perusahaan dapat dibagi menjadi dua bagian yaitu bagian gudang dan bagian produksi dimana bagian gudang menjadi pendukung dari bagian produksi. Bagian gudang terdiri dari gudang sortir, gudang rangka, dan gudang mesin. Dalam mengalokasikan komponen yang disimpan, perusahaan menggunakan kebijakan penyimpanan random/acak yang berakibat tidak standarnya waktu yang dibutuhkan untuk mencari komponen dan bertambahnya waktu perjalanan operator dalam menyuplai komponen. Penelitian ini memberikan alternatif perancangan tata letak penyimpanan komponen yang dilakukan dengan memperhatikan komponen itu sendiri, dalam hal ini disebut faktor komoditi yang terdiri atas popularity, similarity, characteristic, dan size. Selain berdasarkan komponen yang disimpan, perancangan tata letak penyimpanan ini juga memperhatikan kondisi ruangan yang tersedia. Hasil pengolahan data dan analisis yang dilakukan menunjukkan bahwa tata letak penyimpanan komponen yang terbaik adalah berdasarkan kriteria process similarity. Tata letak penyimpanan komponen berdasarkan kriteria process similarity ini merupakan tata letak penyimpanan komponen terbaik. Kata Kunci : komponen, faktor komoditi, lost time, waktu perjalanan

  8. Perencanaan Tata Kelola Teknologi Informasi Berdasarkan Framework CobiT (Studi Kasus Pada Direktorat Metrologi)

    OpenAIRE

    Falahah, Falahah

    2006-01-01

    Tatakelola Teknologi Informasi (TI) merupakan salah satu aspek penting dari tatakelola Perusahaan secara keseluruhan. Pengelolaan TI yang baik akan menjamin efisiensi dan pencapaian kualitas layanan yang baik bagi tujuan bisnis Perusahaan. Penerapan tata kelola ini harus direncanakan dengan baik agar dapat diimplementasikan sesuai dengan kondisi dan kemampuan Perusahaan.Salah satu kerangka kerja tatakelola TI adalah CobiT. Dalam dokumentasi resminya CobiT juga disertai dengan serangkaian pedo...

  9. PENERAPAN METODE TUTOR SEBAYA UNTUK MENINGKATKAN KETERAMPILANPREPARE DANTABLE SET UP DALAM MATA KULIAH TATA HIDANG

    Directory of Open Access Journals (Sweden)

    Wahyuningsih Wahyuningsih

    2011-02-01

    Full Text Available This learning teching research was intended to improve the learning outcomes of the students practicing in the subject of prepare and table set up, the main subject of Tata Hidang I (Servicing Arragements I. The subject for this research consisted of 18 S1 students of the learning group number 02 for the study Program for PKK, concentrating on Tata Boga, Departement of Service & Production Technology, the Technical Faculty of Semarang State University for the academic period of 2009-2010. This research was conducted in the gasal semester of the academic year of 2009-2010. The needed data were collected by means of tests and obsevation on the learning outcomes of the students practicing in the sub-subject of Prepare and Table Set up. The data collection used grading sheets for practices in Tata Hidang as its instrument. The data were then analized using the method of descriptive presentation. This was a classroom-action research planned in three cycles. The second and third cycles were implemented based on reflections on the first cycle and on improvements that should be reached. Every cycle consisted of two face to face encounters and of 4 activities: (1planning, (2 implementations, (3 observations, (4 reflections. The results of this research showed that the use of peeer tutorials for the learning-teching pocesses had positive effects both on the students and the lecturers that it might improve the quality of lecturing for the Tata Hidang I. The grades obtained by the students from practicing in the sub-subject of prepare and table set up increased from the lowest grade C (46.7% BC and B (60% and AB (26.6 %, and AB (26.6%.. Trough the three cycles, the number of BC grade decreased from 66% to only 20%. Based on the results of this research, it is recommended that peer tutorial for the subject of Tata Hidang I and Servicing in Food & Beverage should be continued in order that the educational serveices provided to the practicing students might be

  10. Crustin protein Amk1 from black tiger shrimp (Penaeus monodon inhibits Vibrio harveyi and Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Moltira Tonganunt1*

    2008-05-01

    Full Text Available A crustin gene (Amk1 was identified from a haemocyte library of the black tiger shrimp, Penaeus monodon. The full-length cDNA consists of 411 bp encoding a deduced precursor of 136 amino acids with a signal peptide of 17 aminoacids. Amk1 contains a hydrophobic and a Gly-rich region at the N-terminus and a 12 conserved cysteine domain (6-DSC at the C-terminus. Transcripts of Amk1 are mainly detected in haemocytes and gills by RT-PCR analysis. A recombinant Amk1was overexpressed and purified from Escherichia coli. This has a molecular mass of 43.66 kDa with a predicted pI of 8.23. Antibacterial assays demonstrated that recombinant Amk1 exhibited antibacterial activity against Gram-positive and Gramnegativebacteria with strong inhibition against Staphylococcus aureus and Vibrio harveyi.

  11. Past and present population dynamics of narwhals Monodon monoceros

    DEFF Research Database (Denmark)

    Garde, Eva

    The narwhal, Monodon monoceros, is a medium-sized odontocete whale, endemic to the Atlantic sector of the Arctic and sub-Arctic. In both Canada and Greenland, subsistence hunting of narwhals by the Inuit has existed for centuries and still today the narwhals are hunted for their precious tusks...... of both marine and terrestrial mammals, although the technique still requires both optimization and further validation. In addition, the thesis includes a study of past population dynamics in narwhals. We have investigated the genetic response to climate change and the emographic history of the narwhal...... as 50.000 years. The results are compared with results from the beluga whale, Delphinapterus leucas, revealing two very different demographic histories....

  12. The effect of gamma irradiation on physical and nutritional quality of Penaeus Monodon shrimps

    International Nuclear Information System (INIS)

    Lacroix, M.L.; Jobin, M.; Latreille, B.; Nouchpramool, K.; Gagnon, M.

    1995-01-01

    Two lots of frozen precooked shrimps from Thailand ''PENAEUS MONODON'' Black tiger variety were irradiated at 1.8 to 3.6 kGy. This way, it was hoped to compare the effects of gamma irradiation on the chemical and physical qualities of frozen precooked shrimps after transportation from Thailand to Canada. The results indicated that the color measurement expressed in terms of lightness (L*) showed a relative stability during storage of processed shrimps. However, based on the value (θ) a more intense red color was observed for the control and the reference. The cooked irradiated and nonirradiated shrimps presented a significant decrease (p ≤ 0.01) of 22% in firmness during the first week of storage. Thus, irradiation did not impair the firmness of shrimps after treatment or during storage. Also, based on the statistical analysis, the irradiation treatment did not affect the content of amino acids of precooked shrimps. (Author)

  13. Isolation and molecular identification of planctomycete bacteria from postlarvae of the giant tiger prawn, Penaeus monodon.

    Science.gov (United States)

    Fuerst, J A; Gwilliam, H G; Lindsay, M; Lichanska, A; Belcher, C; Vickers, J E; Hugenholtz, P

    1997-01-01

    Bacteria phenotypically resembling members of the phylogenetically distinct planctomycete group of the domain Bacteria were isolated from postlarvae of the giant tiger prawn, Penaeus monodon. A selective medium designed in the light of planctomycete antibiotic resistance characteristics was used for this isolation. Planctomycetes were isolated from both healthy and monodon baculovirus-infected prawn postlarvae. The predominant colony type recovered from postlarvae regardless of viral infection status was nonpigmented. Other, less commonly observed types were pink or orange pigmented. A planctomycete-specific 16S rRNA-directed probe was designed and used to screen the isolates for their identity as planctomycetes prior to molecular phylogenetic characterization. 16S rRNA genes from nine prawn isolates together with two planctomycete reference strains (Planctomyces brasiliensis and Gemmata obscuriglobus) were sequenced and compared with reference sequences from the planctomycetes and other members of the domain Bacteria. Phylogenetic analyses and sequence signatures of the 16S rRNA genes demonstrated that the prawn isolates were members of the planctomycete group. Five representatives of the predominant nonpigmented colony type were members of the Pirellula group within the planctomycetes, as were three pink-pigmented colony type representatives. Homology values and tree topology indicated that representatives of the nonpigmented and pink-pigmented colony types formed two discrete clusters within the Pirellula group, not identical to any known Pirellula species. A sole representative of the orange colony type was a member of the Planctomyces group, virtually identical in 16S rDNA sequence to P. brasiliensis, and exhibited distinctive morphology. PMID:8979353

  14. Moult-inhibiting fusion protein augments while polyclonal antisera attenuate moult stages and duration in Penaeus monodon.

    Science.gov (United States)

    Vrinda, S; Jasmin, C; Sivakumar, K C; Jose, Blessy; Philip, Rosamma; Bright Singh, I S

    2016-07-01

    Moulting in crustaceans is regulated by moult-inhibiting hormone (MIH) of the CHH family neuropeptides. The inhibitory functions of MIH have pivotal roles in growth and reproduction of Penaeus monodon. In this study, we report the expression of a thioredoxin-fused mature MIH I protein (mf-PmMIH I) of P. monodon in a bacterial system and its use as antigen to raise polyclonal antiserum (anti-mf-PmMIH I). The mature MIH I gene of 231bp, that codes for 77 amino acids, was cloned into the Escherichia coli thioredoxin gene fusion expression system. The translation expression vector construct (mf-PmMIH I+pET32a+) upon induction produced 29.85kDa mature MIH I fusion protein (mf-PmMIH I). The purified fusion protein was used as exogenous MIH I and as antigen to raise polyclonal antisera. When fusion protein (mf-PmMIH I) was injected into D2 and D3 stages of juvenile shrimp, the moult cycle duration was extended significantly to 16.67±1.03 and 14.67±1.03days respectively compared to that of 11.67±1.03days in controls. Moult duration was further reduced to 8.33±0.82days when polyclonal antiserum (anti-mf-PmMIH I - 1:500 dilutions) was injected. Anti-mf-PmMIH I immunolocalized MIH I producing neurosecretory cells in the eyestalk of P. monodon. In short, the present manuscript reports an innovative means of moult regulation in P. monodon with thioredoxin fused MIH I and antisera developed. Copyright © 2016 Elsevier Inc. All rights reserved.

  15. Abundance and composition of benthic fauna in Penaeus monodon Fabricius culture pond on the west coast of Malaysia peninsular

    OpenAIRE

    Abu Hena, M.K.; Hishamuddin, O.; Misri, K.

    2006-01-01

    This paper reports a study on the benthic faunal abundance and diversity of tiger shrimp P. monodon culture ponds in Perak, west coast of Malaysia Peninsular. Sampling was carried out at three weeks interval throughout the 116 days culture period. In addition, water temperature, dissolved oxygen, salinity, transparency, pH and organic matter of soil were also measured. Results showed that the major groups of macro-benthos comprised of gastropod, foraminifera, polychaetes, bivalve and insects;...

  16. Comparison of ovarian maturation and spawning after unilateral eyestalk ablation of wild-caught and pond-reared Penaeus monodon

    Energy Technology Data Exchange (ETDEWEB)

    Wen, W.; Yang, Q.; Ma, Z.; Jiang, S.; Qiu, L.; Huang, J.; Zhou, F.; Qin, J.G.

    2015-07-01

    The present study compares the efficiency of ovarian maturation and spawning success between wild-caught and pond-reared Penaeus monodon females after unilateral eyestalk ablation. The earliest spawning time after eyestalk ablation was 5.9 days in wild-caught females, which is significantly shorter than the spawning time in pond-reared females (10.5 days). Both wild-caught and pond-reared females repeatedly spawned after eyestalk ablation. On average, each wild-caught female spawned 2.94 times while each pond-reared female spawned only 1.09 times. The spawning induction rate, egg hatching rate, and the number of eggs per spawning were significantly greater in wild-caught females than in pond-reared females. However, the egg size was not significantly different between wild-caught and pond-reared females. Four shrimp sizes (60, 80, 100 and 120 (± 1.0) g) were tested in this study and body weight significantly affected ovarian induction in pond-reared females but not in wild-caught females. Within the same body-weight class, the egg number per spawn in wild-caught females was significantly greater than that in pond-reared females. The egg production per spawn of the pond-reared females in the 120-g size group was two times higher than that in the pond-reared females in the 80-g size group. In conclusion, the fecundity of wild-caught P. monodon females is significantly higher than that of pond-reared P. monodon females. In breeding pond-reared P. monodon, the recommended minimum body weight of females is over 80 g, and the desirable body weight is over 100 g. (Author)

  17. Persistence of Penaeus stylirostris densovirus delays mortality caused by white spot syndrome virus infection in black tiger shrimp (Penaeus monodon)

    Science.gov (United States)

    2013-01-01

    Background Persistent infection of Penaeus stylirostris densovirus (PstDNV) (also called IHHNV) and its non-infectious inserts in the black tiger shrimp, Penaeus monodon (P. monodon) genome are commonly found without apparent disease. Here, we introduced the method of multiplex PCR in order to differentiate shrimp with viral inserts from ones with the infectious virus. The method allowed us to study the effect of pre-infection of IHHNV, in comparison to IHHNV inserts, on WSSV resistance in P. monodon. Results A multiplex PCR system was developed to amplify the entire IHHNV genome, ensuring the accurate diagnosis. Field samples containing IHHNV DNA templates as low as 20 pg or equivalent 150 viral copies can be detected by this method. By challenging the two groups of diagnosed shrimp with WSSV, we found that shrimp with IHHNV infection and those with viral inserts responded to WSSV differently. Considering cumulative mortality, average time to death of shrimp in IHHNV-infected group (day 14) was significantly delayed relative to that (day 10) of IHHNV-inserted group. Real-time PCR analysis of WSSV copy number indicated the lower amount of WSSV in the IHHNV-infected group than the virus-inserted group. The ratio of IHHNV: WSSV copy number in all determined IHHNV-infected samples ranged from approximately 4 to 300-fold. Conclusion The multiplex PCR assay developed herein proved optimal for convenient differentiation of shrimp specimens with real IHHNV infection and those with insert types. Diagnosed shrimp were also found to exhibit different WSSV tolerance. After exposed to WSSV, the naturally pre-infected IHHNV P. monodon were less susceptible to WSSV and, consequently, survived longer than the IHHNV-inserted shrimp. PMID:23414329

  18. Antimicrobial effects of essential oils of Cinnamosma fragrans on the bacterial communities in the rearing water of Penaeus monodon larvae.

    Science.gov (United States)

    Sarter, Samira; Randrianarivelo, Roger; Ruez, Philippe; Raherimandimby, Marson; Danthu, Pascal

    2011-04-01

    Farmed shrimps are vectors of various Vibrio species that are considered a potential health hazard. Previous study has shown that Vibrio parahaemolyticus, Vibrio vulnificus, and Vibrio alginolyticus dominated in the water and larval samples of shrimp hatchery (Randrianarivelo et al. 2010 ). The effects of two essential oils (EOs) of Cinnamosma fragrans, an endemic plant to Madagascar (B8: linalool-type and B143: 1,8-cineole-type), were determined on the total heterotrophic aerobic bacteria and the Vibrio concentrations in the rearing water of Penaeus monodon hatchery. The assays took place in OSO Farming's shrimp hatchery in Madagascar. EOs were directly added to the water tank. The bacterial concentrations of water tank were assessed on marine agar and thiosulfate citrate bile sucrose agar. The larvae culture corresponded to four replicates each of B8, B143, erythromycin (E), and control (oil and antibiotic free). The bacterial concentration of the rearing water in B8, B143, and antibiotic (E) tanks were significantly lower (p  0.05) between the three treatments B8, B143, and E. This study demonstrated that both EOs of C. fragrans, like antibiotic, inhibited bacterial growth in the rearing water of P. monodon larvae. The potential of C. fragrans EO to control the bacterial load in in vivo conditions of P. monodon hatchery makes it a relevant option for producers to minimize risk of Vibrio growth in the rearing water of larvae, which is the primary source of colonization of shrimp larvae.

  19. XBP1 (X-Box-Binding Protein-1)-Dependent O-GlcNAcylation Is Neuroprotective in Ischemic Stroke in Young Mice and Its Impairment in Aged Mice Is Rescued by Thiamet-G.

    Science.gov (United States)

    Jiang, Meng; Yu, Shu; Yu, Zhui; Sheng, Huaxin; Li, Ying; Liu, Shuai; Warner, David S; Paschen, Wulf; Yang, Wei

    2017-06-01

    Impaired protein homeostasis induced by endoplasmic reticulum dysfunction is a key feature of a variety of age-related brain diseases including stroke. To restore endoplasmic reticulum function impaired by stress, the unfolded protein response is activated. A key unfolded protein response prosurvival pathway is controlled by the endoplasmic reticulum stress sensor (inositol-requiring enzyme-1), XBP1 (downstream X-box-binding protein-1), and O-GlcNAc (O-linked β-N-acetylglucosamine) modification of proteins (O-GlcNAcylation). Stroke impairs endoplasmic reticulum function, which activates unfolded protein response. The rationale of this study was to explore the potentials of the IRE1/XBP1/O-GlcNAc axis as a target for neuroprotection in ischemic stroke. Mice with Xbp1 loss and gain of function in neurons were generated. Stroke was induced by transient or permanent occlusion of the middle cerebral artery in young and aged mice. Thiamet-G was used to increase O-GlcNAcylation. Deletion of Xbp1 worsened outcome after transient and permanent middle cerebral artery occlusion. After stroke, O-GlcNAcylation was activated in neurons of the stroke penumbra in young mice, which was largely Xbp1 dependent. This activation of O-GlcNAcylation was impaired in aged mice. Pharmacological increase of O-GlcNAcylation before or after stroke improved outcome in both young and aged mice. Our study indicates a critical role for the IRE1/XBP1 unfolded protein response branch in stroke outcome. O-GlcNAcylation is a prosurvival pathway that is activated in the stroke penumbra in young mice but impaired in aged mice. Boosting prosurvival pathways to counterbalance the age-related decline in the brain's self-healing capacity could be a promising strategy to improve ischemic stroke outcome in aged brains. © 2017 American Heart Association, Inc.

  20. PENERAPAN STANDAR PELAYANAN MINIMAL PEGAWAI TATA USAHA DI SMP NEGERI SE-KOTA SALATIGA

    Directory of Open Access Journals (Sweden)

    Aan Ikhsananto

    2016-06-01

    Full Text Available Pegawai tata usaha memiliki tugas-tugas pelayanan yang berhubungan dengan banyak orang di lingkungan sekolah. Observasi awal yang dilakukan peneliti menunjukkan bahwa penerapan Standar Pelayanan Minimal (SPM masih kurang. Melalui peningkatan kompetensi pegawai diharapkan dapat memenuhi standar pelayanan minimal pegawai tata usaha di sekolah. Pemanfaatan fasilitas kantor juga diharapkan dapat menunjang standar pelayanan minimal. Selain itu ketersediaan teknologi informasi diharapkan penerapan standar pelayanan minimal dapat semakin meningkat. Tujuan dari penelitian ini adalah untuk mengetahui apakah kompetensi pegawai, fasilitas kantor, dan teknologi infomasi berpengaruh terhadap penerapan SPM pegawai tata usaha.Variabel dalam penelitian ini adalah kompetensi pegawai (X1, fasilitas kantor (X2 dan teknologi informasi (X3 sebagai variabel bebas serta standar pelayanan minimal (Y sebagai variabel terikatnya. Sampel dalam penelitian ini adalah seluruh pegawai tata usaha (TU di SMP Negeri Se-Kota Salatiga, berjumlah 54 pegawai. Metode pengumpulan data yang digunakan adalah metode dokumentasi, wawancara, dan kuesioner. Teknik analisis data dapat menggunakan regresi berganda bantuan SPSS, asumsi klasik, dan analisis deskriptif persentase. Hasil penelitian ini dapat disimpulkan bahwa kompetensi pegawai, fasilitas kantor, dan teknologi informasi berpengaruh positif dan signifikan terhadap penerapan standar pelayanan minimal pegawai. Clerical employee has service tasks associated with many people in the school environment. Preliminary observations conducted by researchers showed that the application of the Minimum Service Standards (SPM is still lacking. By improving the competence of employees is expected to meet minimum service standards in school administration employees. The usefullness of office facilities are also expected to support the minimum service standards. Besides the availability of information technology is expected implementation of

  1. Proses Penanganan Tata Kearsipan Daerah pada Kantor Perpustakaan Arsip dan Dokumentasi Deli Serdang

    Directory of Open Access Journals (Sweden)

    Lisnawati Lisnawati

    2014-06-01

    Full Text Available Kebutuhan manusia akan informasi begitu penting, beragamnya jenis media saluran informasi turut menambah kemudahan bagi siapa saja untuk mencari informasi tidak terkecuali bagi pemerintahan daerah sebagai penyelenggara pemerintahan memiliki peran penting untuk menyediakan sistem pengarsipan dalam mendukung kinerja. Tujuan penelitian ini untuk melihat bagaimanakah proses penanganan tata kearsipan daerah pada kantor perpustakaan arsip dan dokumentasi Kabupaten Deli Serdang. Metode penelitian yang digunakan adalah berjenis kualitatif dengan pendekatan deskriptif dimana pencarian data menggunakan data primer dan data skunder. Hasil penelitian memperlihatkan peralatan penyimpanan arsip masih mengunakan peralatan sederhana dan belum berbasis teknologi.

  2. The enrichment of TATA box and the scarcity of depleted proximal nucleosome in the promoters of duplicated yeast genes.

    Science.gov (United States)

    Kim, Yuseob; Lee, Jang H; Babbitt, Gregory A

    2010-01-01

    Population genetic theory of gene duplication suggests that the preservation of duplicate copies requires functional divergence upon duplication. Genes that can be readily modified to produce new gene expression patterns may thus be duplicated often. In yeast, genes exhibit dichotomous expression patterns based on their promoter architectures. The expression of genes that contain TATA box or occupied proximal nucleosome (OPN) tends to be variable and respond to external signals. On the other hand, genes without TATA box or with depleted proximal nucleosome (DPN) are expressed constitutively. We find that recent duplicates in the yeast genome are heavily biased to be TATA box containing genes and not to be DPN genes. This suggests that variably expressed genes, due to the functional organization in their promoters, have higher duplicability than constitutively expressed genes.

  3. QTL for white spot syndrome virus resistance and the sex-determining locus in the Indian black tiger shrimp (Penaeus monodon).

    Science.gov (United States)

    Robinson, Nicholas A; Gopikrishna, Gopalapillay; Baranski, Matthew; Katneni, Vinaya Kumar; Shekhar, Mudagandur S; Shanmugakarthik, Jayakani; Jothivel, Sarangapani; Gopal, Chavali; Ravichandran, Pitchaiyappan; Gitterle, Thomas; Ponniah, Alphis G

    2014-08-28

    Shrimp culture is a fast growing aquaculture sector, but in recent years there has been a shift away from tiger shrimp Penaeus monodon to other species. This is largely due to the susceptibility of P. monodon to white spot syndrome virus disease (Whispovirus sp.) which has impacted production around the world. As female penaeid shrimp grow more rapidly than males, mono-sex production would be advantageous, however little is known about genes controlling or markers associated with sex determination in shrimp. In this study, a mapped set of 3959 transcribed single nucleotide polymorphisms were used to scan the P. monodon genome for loci associated with resistance to white-spot syndrome virus and sex in seven full-sibling tiger shrimp families challenged with white spot syndrome virus. Linkage groups 2, 3, 5, 6, 17, 18, 19, 22, 27 and 43 were found to contain quantitative trait loci significantly associated with hours of survival after white spot syndrome virus infection (P shrimp.

  4. Method of quantitative x-ray diffractometric analysis of Ta-Ta2C system

    International Nuclear Information System (INIS)

    Gavrish, A.A.; Glazunov, M.P.; Korolev, Yu.M.; Spitsyn, V.I.; Fedoseev, G.K.

    1976-01-01

    The syste86 Ta-Ta 2 C has beemonsidered because of specific features of diffraction patterns of the components, namely, overlapping of the most intensive reflexes of both phases. The method of standard binary system has been used for quantitative analysis. Because of overlapping of the intensive reflexes dsub(1/01)=2.36(Ta 2 C) and dsub(110)=2.33(Ta), the other, most intensive, reflexes have been used for quantitative determination of Ta 2 C and Ta: dsub(103)=1.404 A for tantalum subcarbide and dsub(211)=1.35A for tantalum. Besides, the TaTa 2 C phases have been determined quantitatively with the use of another pair of reflexes: dsub(102)=1.82 A for Ta 2 C and dsub(200)=1.65 A for tantalum. The agreement between the results obtained while performing the quantitative phase analysis is good. To increase reliability and accuracy of the quantitative determination of Ta and Ta 2 C, it is expedient to carry out the analysis with the use of two above-mentioned pairs of reflexes located in different regions of the diffraction spectrum. Thus, the procedure of quantitative analysis of Ta and Ta 2 C in different ratios has been developed taking into account the specific features of the diffraction patterns of these components as well as the ability of Ta 2 C to texture in the process of preparation

  5. Environmental control measures in sponge iron industry with particular reference to Tata Sponge Iron Limited

    Energy Technology Data Exchange (ETDEWEB)

    Sarangi, B.M.; Kesav, B.; Sheshadri, M.K.

    2002-07-01

    Direct reduced iron or sponge iron technology was developed as an alternate route for steel making and is considered as a clean technology. The waste generation and gas emissions from this route of steel making are far less when compared to the conventional blast furnace route. The paper details the efforts of Tata Sponge Iron Limited to make the process a clean technology without affecting its surroundings and natural resources. The paper describes the system of bag filters made from filter glass fabric for collecting hot waste gases and for dedusting of product and raw material handling circuits. Design of the plant for waste gas cleaning by scrubbers and by electrostatic precipitators is described. Major pollution control equipment installed at Tata Sponge Iron Ltd., described in the article are: a wet gas cleaning plant (in 1986), a second gas cleaning plant with electrostatic precipitator (in 1998) and dust extraction and dust suppression systems. Water is sprayed around the plant to control fugitive dust and trees have been grown around it. 13 figs.

  6. Effect of guava leaves on growth and the non-specific immune response of Penaeus monodon.

    Science.gov (United States)

    Yin, Xiao-Li; Li, Zhuo-Jia; Yang, Keng; Lin, Hei-Zhao; Guo, Zhi-Xun

    2014-09-01

    Guava (Psidium guajava L.) leaf extracts have antiviral and antibacterial activity against shrimp pathogens such as yellow-head virus (YHV), white spot syndrome virus (WSSV), and Vibrio harveyi, which make it a potential water disinfectant for use in shrimp culture. In this study, the safety of guava leaf supplementation in shrimp was evaluated by studying its influence on growth and the non-specific immune response of Penaeus monodon. Six diets containing different levels of guava leaves (0% [basal diet], 0.025% [G1], 0.05% [G2], 0.1% [G3], 0.2% [G4], and 0.4% [G5]) were fed to groups of shrimp (1.576 ± 0.011 g body weight) in triplicate for 56 days. Growth performance (final body weight, WG, PWG, SGR) of shrimp fed guava leaf diets was significantly higher (P 0.05) were found. Dietary supplementation with guava leaf improved the activities of prophenoloxidase (PO) and nitric oxide synthase (NOS) in serum, and of superoxide dismutase (SOD), acid phosphatase (ACP), alkaline phosphatase (AKP), and lysozyme (LSZ) both in serum and hepatopancreas of shrimp. In the experimental groups, the activities of these enzymes followed a similar pattern of change; they increased initially at low levels of dietary supplementation and then decreased with increasing concentrations of dietary guava leaf. Serum PO and SOD activities in shrimp fed the G1 diet reached 7.50 U ml(-1) and 178.33 U ml(-1), respectively, with PO activity being significantly higher than in controls. In shrimp fed the G1 diet, SOD, ACP, and AKP activities in hepatopancreas were significantly higher than in the controls, reaching 57.32 U g(-1), 23.28 U g(-1), and 19.35 U g(-1) protein, respectively. The highest activities of serum ACP, AKP, LSZ, and of hepatopancreas LSZ, were observed in the G3 diet group. Total nitric oxide synthase (TNOS) activity was highest (64.80 U ml(-1)) in the G4 diet group, which was significantly higher than that observed in the control group. These results suggest that dietary

  7. El tratamiento de la metáfora en la traducción de Historias de Tata Mundo (The Treatment of the Metaphor in the Translation of Historias de Tata Mundo

    Directory of Open Access Journals (Sweden)

    Jacqueline Araya Ríos

    2016-09-01

    Full Text Available Fabián Dobles plantea mediante hechos característicos y regionalismos una imagen del campesino nacional de mediados de siglo xx en su obra Historias de Tata Mundo. Joan Henry lo traduce al inglés en 1998 y lo titula The Stories of Tata Mundo, transportando al campesino costarricense a la escena de habla inglesa. Se analizan las decisiones de la traductora sobre el tratamiento de las metáforas, teniendo en cuenta el lenguaje figurado como una herramienta empleada por el autor para caracterizar la vida rural del campesinado costarricense. Using traditional activities and regionalisms, Fabián Dobles reconstructed the image of the Costa Rican country folk of the mid-twentieth century in his literary work Historias de Tata Mundo. Joan Henry translated it in 1998, with the title The Stories of Tata Mundo, transporting the local peasant to the English-speaking scenario. The translator’s decisions are analyzed here regarding the translation of metaphors, taking into account figurative speech as a tool used by the author to characterize and depict the rural life of Costa Rican country folk.

  8. Gamma radiation effects on bacterial contamination and organoleptic characteristics of frozen Ponaeus Monodon

    International Nuclear Information System (INIS)

    Motamedi Sedeh, F.; Afsharian, R.; Zolfagarieh, H.; Nikbakht, M.; Shafaee, S. K.; Ayazi, M.; Fatolahi, H.; Moharami, A.; Babaee, M.

    2010-01-01

    The aim of this study was the application of irradiation process to decrease bacterial contamination of the penaeus monodon. The shrimp samples were obtained from Hormozgan and were sent to the microbiological laboratory. Bacterial contamination of shrimp were determined by counting the aerobic mesophil bacteria, Staphylococcus areus, Coliforms, Vibrio. parahaemolyticus and Salmonella. The shrimp samples were irradiated at different doses of gamma ray. Finally, the optimum dose of the gamma ray for bacterial decontamination of shrimp, especially of Vibrio Parahaemolyticus, was obtained to be 2 kGy. Also, the chemical factors of irradiated and non-irradiated samples such as Protein, Fat, total volatile Nitrogen, Non Protein Nitrogen, Peroxide Value and Amino Acids were measured. There were not any important difference among them. Also, there were not any significant difference between total volatile Nitrogen and Peroxide Value (P>0.05) for the irradiated and non-irradiated shrimp samples. Study of bacterial contaminations of the irradiated and non irradiated samples after 12 months showed that irradiation by 2 kGy can control the microbial contaminations. Four types of films for packaging: Pet.pe, Pp.pe, Bopp, and Pe.20 were used for storage of the irradiated and non irradiated shrimp samples in-18 d egree C during 12 months. All of the packages were studied on the aspects of color, odor, tissue and taste of shrimp samples and elasticity and pressing of packages. For the irradiated and non irradiated shrimp Pet.pe and Pp.pe were preferred, respectively.

  9. Acute Toxicity and Neurotoxicity of Chlorpyrifos in Black Tiger Shrimp, Penaeus monodon

    Directory of Open Access Journals (Sweden)

    Tassanee Eamkamon

    2012-01-01

    Full Text Available Acute toxicity and neurotoxicity of chlorpyrifos were determined in black tiger shrimp, P. monodon. LC50 values after 24 to 96 h of exposure were between 149.55 and 59.16 nmol/L. To determine the neurotoxicity of chlorpyrifos, the inhibition of acetylcholinesterase was monitored in the gill of the shrimps exposed to lethal (0.019, 0.194, and 1.942 µmol/L and sub-lethal (0.019, 0.194, and 1.942 nmol/L concentrations of chlorpyrifos. In lethal dose exposure, the AChE activities observed in shrimp exposed to 0.194, and 1.942 µmol/L of chlorpyrifos were significantly lower (1.7 and 3.3 times than that of control shrimp after 30 min of exposure (p<0.05. In sub-lethal exposure tests, the AChE activity of shrimp was significantly lower (1.9 times than that of control shrimp after exposure to 1.942 nmol/L of chlorpyrifos for 72 h (p<0.05. The sensitive reduction of AChE activity at the sub-lethal concentration, which was 30 times lower than 96 h LC50 value found in this study, indicates the potential use as a biomarker of chlorpyrifos exposure.

  10. Induction of ovarian maturation in Penaeus monodon by molecular signal interventional approach.

    Science.gov (United States)

    Devaraj, Halagowder; Saravanakumar, Marimuthu; Thiyagu, Mani

    2012-11-01

    Vitellogenin (VTG) synthesis in the hepatopancreas and ovary is negatively regulated by vitellogenesis-inhibiting hormone (VIH) produced in the neurosecretory cell of X-organ/sinus gland complex of the eyestalks of penaeid shrimp. Eyestalk ablation is used commercially to induce ovarian maturation in shrimps which leads to an eventual loss of the spawner. The aim of the present study was to understand the molecular mechanism of VIH regulation in ovarian development and its inhibition of VTG gene expression by using a MEK-specific inhibitor (U0126). The real-time quantitative PCR results showed VTG mRNA level was progressively increased in the ovary and hepatopancreas of unilateral eyestalk-ablated and inhibitor-treated shrimps. Western blot analysis also showed that phosphoMEK was detected only in the unilateral eyestalk-ablated and control shrimp, whereas phospho-MEK was not detected in inhibitor-treated shrimp. DAX-1, SF-1, and StAR expression correlated with changes in VIH mRNA and altered phospho-ERK levels. This is consistent with the hypothesis that suppression of DAX-1 results in SF-1-mediated StAR protein upregulation of estradiol that is implicated in vitellogenesis. This is the first report that demonstrates the molecular mechanism of VIH suppression via MEK pathway to induce ovarian maturation in female Penaeus monodon by molecular signal intervention, a less-invasive method than traditional eyestalk ablation. Copyright © 2012 Wiley Periodicals, Inc.

  11. Histological and three dimensional organizations of lymphoid tubules in normal lymphoid organ of Penaeus monodon.

    Science.gov (United States)

    Duangsuwan, Pornsawan; Phoungpetchara, Ittipon; Tinikul, Yotsawan; Poljaroen, Jaruwan; Wanichanon, Chaitip; Sobhon, Prasert

    2008-04-01

    The normal lymphoid organ of Penaeus monodon (which tested negative for WSSV and YHV) was composed of two parts: lymphoid tubules and interstitial spaces, which were permeated with haemal sinuses filled with large numbers of haemocytes. There were three permanent types of cells present in the wall of lymphoid tubules: endothelial, stromal and capsular cells. Haemocytes penetrated the endothelium of the lymphoid tubule's wall to reside among the fixed cells. The outermost layer of the lymphoid tubule was covered by a network of fibers embedded in a PAS-positive extracellular matrix, which corresponded to a basket-like network that covered all the lymphoid tubules as visualized by a scanning electron microscope (SEM). Argyrophilic reticular fibers surrounded haemal sinuses and lymphoid tubules. Together they formed the scaffold that supported the lymphoid tubule. Using vascular cast and SEM, the three dimensional structure of the subgastric artery that supplies each lobe of the lymphoid organ was reconstructed. This artery branched into highly convoluted and blind-ending terminal capillaries, each forming the lumen of a lymphoid tubule around which haemocytes and other cells aggregated to form a cuff-like wall. Stromal cells which form part of the tubular scaffold were immunostained for vimentin. Examination of the whole-mounted lymphoid organ, immunostained for vimentin, by confocal microscopy exhibited the highly branching and convoluted lymphoid tubules matching the pattern of the vascular cast observed in SEM.

  12. PENGARUH PAKAN ALAMI Chaetoceros spp. TERHADAP PERKEMBANGAN DAN SINTASAN LARVA UDANG WINDU, Penaeus monodon

    Directory of Open Access Journals (Sweden)

    Samuel Lante

    2015-09-01

    Full Text Available Pakan alami berupa mikroalga merupakan salah satu faktor pendukung dalam keberhasilan usaha pembenihan udang windu, Penaeus monodon. Penelitian ini bertujuan untuk mengetahui jenis pakan alami, Chaetoceros spp., yang dapat meningkatkan pertumbuhan dan sintasan larva udang windu. Penelitian ini menggunakan 15 buah bak fiber berukuran 60 cm x 40 cm x 50 cm dengan volume 120 liter yang disi air sebanyak 100 liter. Kepadatan naupli adalah 90 ekor/liter atau 9.000 ekor/bak dan lima jenis pakan alami Chaetoceros spp. yang diuji yaitu: Chaetoceros simplex, Chaetoceros ceratosporum, Chaetoceros calcitrans, Chaetoceros amami, dan Chaetoceros gracilis. Hasil penelitian menunjukkan bahwa aplikasi kelima jenis pakan alami memberikan laju perkembangan larva yang relatif sama sampai stadia PL-1. Aplikasi Chaetoceros gracilis memberikan sintasan larva udang windu tertinggi yaitu 41,48% dan dengan Chaetoceros simplex memberikan sintasan larva terendah (28,02%. Hasil penelitian ini mengindikasikan bahwa aplikasi Chaetoceros spp. yang berbeda pada pemeliharaan larva berpengaruh terhadap sintasan larva udang windu, namun tidak berpengaruh terhadap laju metamorfosis larva. Aplikasi Chaetoceros gracilis sebagai pakan alami adalah terbaik untuk sintasan larva udang windu.

  13. Synthesis of silver nanoparticles by coastal plant Prosopis chilensis (L.) and their efficacy in controlling vibriosis in shrimp Penaeus monodon

    Science.gov (United States)

    Kandasamy, Kathiresan; Alikunhi, Nabeel M.; Manickaswami, Gayathridevi; Nabikhan, Asmathunisha; Ayyavu, Gopalakrishnan

    2013-02-01

    The present work investigated the effect of leaf extract from coastal plant Prosopis chilensis on synthesis of silver nanoparticles using AgNO3 as a substrate and to find their antibacterial potential on pathogenic Vibrio species in the shrimp, Penaeus monodon. The leaf extract could be able to produce silver nanoparticles, as evident by gradual change in colour of the reaction mixture consisted of the extract and 1 mM AgNO3 to dark brown. The silver nanoparticles exhibited 2 θ values corresponding to the presence of silver nanocrystal, as evident by X-ray diffraction spectrum. The peaks corresponding to flavanones and terpenoids were found to be stabilizing agents of the nanoparticles, as revealed by Fourier transform infrared spectroscopy. The size of silver nanoparticles ranged from 5 to 25 nm with an average of 11.3 ± 2.1 nm and was mostly of spherical in shape, as confirmed by transmission electron microscopy. The silver nanoparticles were found to inhibit Vibrio pathogens viz., Vibrio cholerae, V. harveyi, and V. parahaemolyticus and this antibacterial effect was better than that of leaf extract, as proved by disc diffusion assay. The nanoparticles were then tested in the shrimp Penaeus monodon challenged with the four species of Vibrio pathogens for 30 days. The shrimps fed with silver nanoparticles exhibited higher survival, associated with immunomodulation in terms of higher haemocyte counts, phenoloxidase and antibacterial activities of haemolymph of P. monodon which is on par with that of control. Thus, the present study proved the possibility of using silver nanoparticles produced by coastal Prosopis chilensis as antibacterial agent in controlling vibriosis.

  14. Prinsip Kehati-Hatian di Bidang Pengelolaan Lingkungan Hidup dalam Peraturan Daerah Rencana Tata Ruang Wilayah Provinsi Maluku

    Directory of Open Access Journals (Sweden)

    La Ode Angga

    2017-06-01

    Full Text Available Prinsip Kehati-Hatian di Bidang Pengelolaan Lingkungan Hidup dalamPeraturan Daerah Rencana Tata Ruang Wilayah Provinsi Maluku bertujuan untuk mengetahui hakekat prinsip kehati-hatian dalam Rencana Tata Ruang Wilayah di Provinsi Maluku.Penelitian ini merupakan penelitian yuridis normatif, yaitu penelitian yang dilakukan terhadap azas-azas hukum, kaedah-kaedah hukum dalam arti nilai (norma peraturan hukum konkrit dan sistem hukum.Pendekatan yang digunakan dalam penelitian adalah pendekaan filosofis (philosophicalapproach, pendekatan konseptual (conseptual approach, pendekatan perundang-undangan (statute approach. Hasil yang diperoleh bahwa prinsip kehati-hatian dalam Perda RTRW Provinsi Maluku No. 16 Tahun 2013 dilakukan pencegahan secara dini, pencegahan antisipatif, kehati-hatian (Prudential Principles terhadap perlindungan dan pengelolaan sumber daya alam dan lingkungan hidup serta penataan ruang. Filosofis pengaturan prinsip kehati-hatian dalam Perda RTRW Provinsi Maluku bahwa mengingat fungsi ruang sebagai tempat manusia dan makhluk hidup lain, melakukan kegiatan, dan memelihara kelangsungan hidupnya, pada dasarnya ketersediaannya tidak tak terbatas. Berkaitan itu untuk mewujudkan rencana tata ruang wilayah Provinsi Maluku yang aman, nyaman, produktif, dan berkelanjutan perlu dilakukan rencana tata ruang wilayah yang dapat mengharmonisasi lingkungan alam dan lingkungan buatan  mampu mewujudkan keterpaduan penggunaan sumber daya alam dan sumber daya buatan, serta dapat memberikan perlindungan terhadap fungsi ruang dan pencegahan dampak negatif terhadap lingkungan hidup akibat pemanfaatan ruang. 

  15. Regulating gonad inhibition and vitellogenin/vitellin induction in Penaeus monodon using mature GIH fusion protein and polyclonal antisera

    Digital Repository Service at National Institute of Oceanography (India)

    Vrinda, S.; Jasmin, C.; Sivakumar, K.C.; Jose, S.; Jose, B.; Philip, R; BrightSingh, I

    September 2016 Available online 15 September 2016 Keywords: Gonad-inhibiting hormone (GIH) Fusion/chimeric proteinaa r t i c l e i n f o b s t r a c tRegulating gonad inhibition and vitellogen Penaeus monodon using mature GIH fusion polyclonal antisera... restriction site overhangs to match with that of the expression vector; pET32a+. The forward primer (GIH-F- 5′-GAAT TCAACATCCTGGACAGCAAATGCAGGGGTGC-3′) contained EcoR I site (italics) and 29 nucleotide residues encoding the N terminal amino acid residues...

  16. Pengaruh getah pepaya (Carica papaya) terhadap sintasan tokolan udang windu (Panaeus monodon) pada kepadatan yang berbeda selama pengangkutan

    OpenAIRE

    Sofyatuddin Karina; Wahyu Tursina

    2013-01-01

    The objective of present study was to evaluate the possibility of papaya’s latex (Carica papaya) as anti stress or larvaside for tiger shrimp (Panaeus monodon) post larvae Pl 14-30 during transportation. The tiger shrimp post larvae was collected from BBAP Ujung Batee, Aceh Besar on September, 2012. Reseach method used the completely random design with two factors, density of post larvae with three treatments (1000; 1500 dan 2000 ind/l) and concentration of papaya’s latex with four treatments...

  17. Aspartic acid racemization rate in narwhal (Monodon monoceros) eye lens nuclei estimated by counting of growth layers in tusks

    DEFF Research Database (Denmark)

    Garde, Eva; Heide-Jørgensen, Mads Peter; Ditlevsen, Susanne

    2012-01-01

    Ages of marine mammals have traditionally been estimated by counting dentinal growth layers in teeth. However, this method is difficult to use on narwhals (Monodon monoceros) because of their special tooth structures. Alternative methods are therefore needed. The aspartic acid racemization (AAR......) technique has been used in age estimation studies of cetaceans, including narwhals. The purpose of this study was to estimate a species-specific racemization rate for narwhals by regressing aspartic acid D/L ratios in eye lens nuclei against growth layer groups in tusks (n=9). Two racemization rates were...

  18. 'iMonitor' - bringing ironmaking online at Tata Steel

    Energy Technology Data Exchange (ETDEWEB)

    Pande, M.; Kumar, A. [Tata Steel, Jamshedpur (India)

    2004-07-01

    This paper explains the integration of data related to iron making in Tata Steel. The entire data is captured in real time which means as and when it is generated. The data is archived in a central database, which uses Oracle 9i. It covers all the processes in the iron making area and useful parameters from laboratories and out-located departments of raw materials area. The data is presented using user friendly visualisation which is deployed on the web by the Oracle Application Server. Some select clients are also distributed an in-house developed software which shows real-time graphical displays in Microsoft Excel. The system named iMonitor thus provides a common platform to view the iron making process as a whole and also provides an opportunity for process monitoring and control through real-time analysis, process models and expert systems.

  19. EFEKTIVITAS MODEL PEMBELAJARAN BERBASIS KOMPETENSI DENGAN PENDEKATAN DUNIA KERJA PADA PROGRAM D3 TATA BOGA

    Directory of Open Access Journals (Sweden)

    Rina Febriana

    2017-02-01

    Full Text Available Abstrak: Penelitian bertujuan untuk menghasilkan model pembelajaran berbasis kompetensi dengan pendekatan dunia kerja (model PKBK, rumusan kompetensi mahasiswa D3 Tata Boga, rancangan pembelajaran berbasis kompetensi, dan rubrik penilaian. Penelitian ini merupakan penelitian dan pengembangan yang mengadopsi model prosedural Borg & Gall.Subjek penelitian adalah praktisi industri perhotelan, mahasiswa proram D3 Tata Boga dan dosen matakuliah praktik. Teknik pengumpulan data lewat FGD dan pemberian angket, sedang teknik analisis data dilakukan secara deskriptif kualitatitif dan statistik deskriptif. Hasil penelitian ini adalah (1 model PKBK memiliki komponen: profil lulusan, learning outcomes, rumusan kompetensi, pengalaman pembelajaran, rancangan pembelajaran, dan evaluasi kompetensi, (2 rumusan kompetensi untuk lulusan terbagi menjadi 2 kelompok yaitu technical skills dan employability skills, (3 rancangan pembelajaran pada model PKBK terdiri dari: struktur kurikulum, materi dan bahan pembelajaran, diagram pencapaian kompetensi dan dokumen kurikulum, (4 rubrik penilaian berisi penilaian terhadap employability dan technical skills. THE EFFECTIVENESS OF A COMPETENCY-BASED LEARNING MODEL USING WORLD OF WORK APPROACH AT D3 FOOD AND NUTRITION PROGRAMME Abstract: This research aims to produce a model of competence-based learning using the world of work approach (model PKBK, to formulate students’ competence, to design competence-based learning, and to develop evaluation rubric. This research adopted Research and Development model developed by Borg and Gall. The research subjects were hotel practitioners, students of D3 Food and Nutrition, and lechturers of practice courses. Data were collected through FGDs and questionnaires and analysed using descriptive qualitative and descriptive stastitics tecniques. The findings show that (1 the PKBK model comprises graduates’ profile, learning outcomes, competence formulation, learning design, and competence

  20. Full trans-activation mediated by the immediate-early protein of equine herpesvirus 1 requires a consensus TATA box, but not its cognate binding sequence.

    Science.gov (United States)

    Kim, Seong K; Shakya, Akhalesh K; O'Callaghan, Dennis J

    2016-01-04

    The immediate-early protein (IEP) of equine herpesvirus 1 (EHV-1) has extensive homology to the IEP of alphaherpesviruses and possesses domains essential for trans-activation, including an acidic trans-activation domain (TAD) and binding domains for DNA, TFIIB, and TBP. Our data showed that the IEP directly interacted with transcription factor TFIIA, which is known to stabilize the binding of TBP and TFIID to the TATA box of core promoters. When the TATA box of the EICP0 promoter was mutated to a nonfunctional TATA box, IEP-mediated trans-activation was reduced from 22-fold to 7-fold. The IEP trans-activated the viral promoters in a TATA motif-dependent manner. Our previous data showed that the IEP is able to repress its own promoter when the IEP-binding sequence (IEBS) is located within 26-bp from the TATA box. When the IEBS was located at 100 bp upstream of the TATA box, IEP-mediated trans-activation was very similar to that of the minimal IE(nt -89 to +73) promoter lacking the IEBS. As the distance from the IEBS to the TATA box decreased, IEP-mediated trans-activation progressively decreased, indicating that the IEBS located within 100 bp from the TATA box sequence functions as a distance-dependent repressive element. These results indicated that IEP-mediated full trans-activation requires a consensus TATA box of core promoters, but not its binding to the cognate sequence (IEBS). Copyright © 2015 Elsevier B.V. All rights reserved.

  1. Full trans–activation mediated by the immediate–early protein of equine herpesvirus 1 requires a consensus TATA box, but not its cognate binding sequence

    Science.gov (United States)

    Kim, Seong K.; Shakya, Akhalesh K.; O'Callaghan, Dennis J.

    2015-01-01

    The immediate-early protein (IEP) of equine herpesvirus 1 (EHV-1) has extensive homology to the IEP of alphaherpesviruses and possesses domains essential for trans-activation, including an acidic trans-activation domain (TAD) and binding domains for DNA, TFIIB, and TBP. Our data showed that the IEP directly interacted with transcription factor TFIIA, which is known to stabilize the binding of TBP and TFIID to the TATA box of core promoters. When the TATA box of the EICP0 promoter was mutated to a nonfunctional TATA box, IEP-mediated trans-activation was reduced from 22-fold to 7-fold. The IEP trans-activated the viral promoters in a TATA motif-dependent manner. Our previous data showed that the IEP is able to repress its own promoter when the IEP-binding sequence (IEBS) is located within 26-bp from the TATA box. When the IEBS was located at 100 bp upstream of the TATA box, IEP-mediated trans-activation was very similar to that of the minimal IE(nt −89 to +73) promoter lacking the IEBS. As the distance from the IEBS to the TATA box decreased, IEP-mediated trans-activation progressively decreased, indicating that the IEBS located within 100 bp from the TATA box sequence functions as a distance-dependent repressive element. These results indicated that IEP-mediated full trans-activation requires a consensus TATA box of core promoters, but not its binding to the cognate sequence (IEBS). PMID:26541315

  2. Homologous genetic recombination in the yellow head complex of nidoviruses infecting Penaeus monodon shrimp.

    Science.gov (United States)

    Wijegoonawardane, Priyanjalie K M; Sittidilokratna, Nusra; Petchampai, Natthida; Cowley, Jeff A; Gudkovs, Nicholas; Walker, Peter J

    2009-07-20

    Yellow head virus (YHV) is a highly virulent pathogen of Penaeus monodon shrimp. It is one of six known genotypes in the yellow head complex of nidoviruses which also includes mildly pathogenic gill-associated virus (GAV, genotype 2) and four other genotypes (genotypes 3-6) that have been detected only in healthy shrimp. In this study, comparative phylogenetic analyses conducted on replicase- (ORF1b) and glycoprotein- (ORF3) gene amplicons identified 10 putative natural recombinants amongst 28 viruses representing all six genotypes from across the Indo-Pacific region. The approximately 4.6 kb genomic region spanning the two amplicons was sequenced for three putative recombinant viruses from Vietnam (genotype 3/5), the Philippines (genotype 5/2) and Indonesia (genotype 3/2). SimPlot analysis using these and representative parental virus sequences confirmed that each was a recombinant genotype and identified a recombination hotspot in a region just upstream of the ORF1b C-terminus. Maximum-likelihood breakpoint analysis predicted identical crossover positions in the Vietnamese and Indonesian recombinants, and a crossover position 12 nt upstream in the Philippine recombinant. Homologous genetic recombination in the same genome region was also demonstrated in recombinants generated experimentally in shrimp co-infected with YHV and GAV. The high frequency with which natural recombinants were identified indicates that genetic exchange amongst genotypes is occurring commonly in Asia and playing a significant role in expanding the genetic diversity in the yellow head complex. This is the first evidence of genetic recombination in viruses infecting crustaceans and has significant implications for the pathogenesis of infection and diagnosis of these newly emerging invertebrate pathogens.

  3. MBV infection in various stages of black tiger shrimp (Penaeus monodon

    Directory of Open Access Journals (Sweden)

    Supamattaya, K.

    2005-02-01

    Full Text Available MBV infection in various stages of Penaeus monodon were studied. In hatcheries, MBV infection was detected early in nauplii stage using PCR technique, whereas rates of infection of 11.15-49.50% were observed in PL1 using histological technique, rising up to 15.26-100% in PL 10. In earthen ponds, the infection in PL15 was initially in the range of 68.68-96.00%. The infection was decreased toward the end of the first, second and third month of rearing period ranging between 13.63-54.83%. The laboratory trial showed that types of feed might affect the rate of MBV infection of larvae. Postlarva fed with artemia showed lowest infection rate at 29.41±7.98%, whereas the infection rates of shrimp fed with minced cockle flesh and commercial feed were 39.09±12.08% and 52.81±11.91, respectively. In stress test trial, a significant MBV infection was detected in the group of larvae that were raised with 25ºC and 34ºC and the salinity at 6 ppt and 18 ppt for 12 hours then rearing in normal condition for 3 days. In the 24 hour-stress trial, and transferred to normal condition for 7 day, the groups that were exposed to stress conditions had significantly higher rates of infection than the control group (p<0.05. The 24 hour - transportationcondition resulted in highest MBV infection rate (73.61±1.25%. From the present study, it was concluded that MBV infection in larvae from hatcheries increases with period of rearing and stress exposure, but the infection tended to decreased with rearing period in earthen pond condition. Proper feeding management and prevention of stress conditions could reduce of MBV infection in black tiger shrimp.

  4. Bacterial population in intestines of the black tiger shrimp (Penaeus monodon under different growth stages.

    Directory of Open Access Journals (Sweden)

    Wanilada Rungrassamee

    Full Text Available Intestinal bacterial communities in aquaculture have been drawn to attention due to potential benefit to their hosts. To identify core intestinal bacteria in the black tiger shrimp (Penaeus monodon, bacterial populations of disease-free shrimp were characterized from intestines of four developmental stages (15-day-old post larvae (PL15, 1- (J1, 2- (J2, and 3-month-old (J3 juveniles using pyrosequencing, real-time PCR and denaturing gradient gel electrophoresis (DGGE approaches. A total of 25,121 pyrosequencing reads (reading length = 442±24 bases were obtained, which were categorized by barcode for PL15 (7,045 sequences, J1 (3,055 sequences, J2 (13,130 sequences and J3 (1,890 sequences. Bacteria in the phyla Bacteroides, Firmicutes and Proteobacteria were found in intestines at all four growth stages. There were 88, 14, 27, and 20 bacterial genera associated with the intestinal tract of PL15, J1, J2 and J3, respectively. Pyrosequencing analysis revealed that Proteobacteria (class Gammaproteobacteria was a dominant bacteria group with a relative abundance of 89% for PL15 and 99% for J1, J2 and J3. Real-time PCR assay also confirmed that Gammaproteobacteria had the highest relative abundance in intestines from all growth stages. Intestinal bacterial communities from the three juvenile stages were more similar to each other than that of the PL shrimp based on PCA analyses of pyrosequencing results and their DGGE profiles. This study provides descriptive bacterial communities associated to the black tiger shrimp intestines during these growth development stages in rearing facilities.

  5. GENETIC DIVERSITY OF Vibrio harveyi ISOLATED FROM TIGER PRAWN Penaeus monodon HATCHERIES AND GROW OUT PONDS

    Directory of Open Access Journals (Sweden)

    Muliani Muliani

    2006-06-01

    Full Text Available ibrio harveyi is known as one among the most harmful bacteria infecting tiger prawn at every stage of its life’s. The present research was aimed to reveal the genetic diversity of Vibrio harveyi isolated from tiger prawn (Penaeus monodon culture. The samples of bacteria were collected from hatchery (brood-stock, larvae, natural feed, artemia, and larval rearing water and grow-out (juveniles, water, shrimp, sediment, plankton, crab, mollusc, microalgae, and wild fish.  The taxonomic identification of Vibrio spp.was performed based on the physiological and biochemical characteristic following the isolation by Thiosulphate Citrate Bile-salt Sucrose Agar (TCBSA media. Amplified Ribosomoal DNA Restriction Analysis (ARDRA for 16S-rRNA analysis and Macrorestriction Fragment Length Polymorphism (MLFP analysis using Pulsed Field Gel Electrophoresis (PFGE were applied to reveal the genetic diversity of V. harveyi. According to the taxonomic identification, of 361 isolates of Vibrio spp., 129 isolates (35.7% were identified as V. harveyi. The result of ARDRA analysis showed that the 16S-rRNA gene of V. harveyi digested by RsaI and HhaI enzyme, each generated three and four identical fragments respectively for the all samples. These meaned that ARDRA could not reveal any genetic variation on V. harveyi. The size range of all DNA fragment was less than 500 bp. This result indicated that the high genetic diversity of V. harveyi was revealed by MFLP-PFGE analysis. DNA fragment of V. harveyi was digested by NotI enzyme.

  6. Bacterial population in intestines of the black tiger shrimp (Penaeus monodon) under different growth stages.

    Science.gov (United States)

    Rungrassamee, Wanilada; Klanchui, Amornpan; Chaiyapechara, Sage; Maibunkaew, Sawarot; Tangphatsornruang, Sithichoke; Jiravanichpaisal, Pikul; Karoonuthaisiri, Nitsara

    2013-01-01

    Intestinal bacterial communities in aquaculture have been drawn to attention due to potential benefit to their hosts. To identify core intestinal bacteria in the black tiger shrimp (Penaeus monodon), bacterial populations of disease-free shrimp were characterized from intestines of four developmental stages (15-day-old post larvae (PL15), 1- (J1), 2- (J2), and 3-month-old (J3) juveniles) using pyrosequencing, real-time PCR and denaturing gradient gel electrophoresis (DGGE) approaches. A total of 25,121 pyrosequencing reads (reading length = 442±24 bases) were obtained, which were categorized by barcode for PL15 (7,045 sequences), J1 (3,055 sequences), J2 (13,130 sequences) and J3 (1,890 sequences). Bacteria in the phyla Bacteroides, Firmicutes and Proteobacteria were found in intestines at all four growth stages. There were 88, 14, 27, and 20 bacterial genera associated with the intestinal tract of PL15, J1, J2 and J3, respectively. Pyrosequencing analysis revealed that Proteobacteria (class Gammaproteobacteria) was a dominant bacteria group with a relative abundance of 89% for PL15 and 99% for J1, J2 and J3. Real-time PCR assay also confirmed that Gammaproteobacteria had the highest relative abundance in intestines from all growth stages. Intestinal bacterial communities from the three juvenile stages were more similar to each other than that of the PL shrimp based on PCA analyses of pyrosequencing results and their DGGE profiles. This study provides descriptive bacterial communities associated to the black tiger shrimp intestines during these growth development stages in rearing facilities.

  7. Application of Irradiated Pro biotic Microorganism in Black Tiger Shrimp (Penaeus monodon Fabricius) Culture

    International Nuclear Information System (INIS)

    Areechon, Nontawith; Purivrojkul, Watchariya; Srisapoome, Prapansak; Piadiang, Nattaya

    2006-09-01

    Marine shrimp culture in Thailand has been developed continuously for the past two decades. This development will ensure the highest level of shrimp quality that will be suitable for the consumption of the people in the country and also aboard. The trend of culture system emphasizes on disease prevention more than treatment which will consequently limit the application of drug and chemicals. Application of pro biotic has been one means of this prevention that are commonly practiced by shrimp farmers. This research was conducted to compare the efficacy of normal Bacillus subtilis isolate from shrimp intestine and an irradiated B. subtilis as a pro biotic in shrimp feed. It was found that overall results were quite the same. These included the broth Co-culture assay. Effects on immune functions were conducted with Penaeus monodon with initial average weight of 17 gms by feeding with 3 gms/kg feed of spore of these two pro biotic for two mouths. The results indicated that both pro biotic caused significant improvement on percent phagocytosis only at the forth week of feeding trial and the overall enhancement of bactericidal activity. However, total haemocyte count and phenoloxidase activity were not altered. Total bacterial count in shrimp intestine was also conducted during the two month trial. the results indicated significant reduction of Vibrio spp. of both pro biotic groups when compared with the control. Number of Bacillus spp. in intestine were continuously high even after pro biotic treatment had been stopped Growth rate of experiment and control shrimp was not significantly different.

  8. ANALYSIS OF IMMUNE RESPONSES ON TRANSGENIC TIGER SHRIMP (Penaeus monodon AGAINST PATHOGENIC BACTERIUM Vibrio harveyi

    Directory of Open Access Journals (Sweden)

    Andi Parenrengi

    2014-06-01

    Full Text Available Vibriosis is one of main diseases of the black tiger shrimp Penaeus monodon infected by pathogenic bioluminous bacterium Vibrio harveyi that can cause mass mortalities in shrimp culture. The bacteria can also trigger the disease white spot syndrome virus (WSSV. An effort to produce shrimp disease-resistant strains has been done through transgenesis technology with antiviral gene transfection. By this technology, it is expected an increase in the immune response of shrimp in a variety of diseasecausing pathogens. This study aimed to determine the immune responses (total haemocytes, haemocyte differentiation, and phenoloxydase activity of transgenic tiger shrimp against pathogenic bacterium V. harveyi. Research using completely randomized design, which consists of two treatments and three replications. Test animals being used were transgenic and non-transgenic shrimp with size, weight 3.93±1.25 g and a total length of 7.59±0.87 cm. Treatments being tested were the injection of bacterium V. harveyi (density of 5x106 cfu/mL of 0.1 mL/individual on transgenic (A and non-transgenic shrimp (B. Immune response parameters such as total haemocytes, haemocyte differentiation, and phenoloxydase activity were observed on day 1, 3, and 6 days after challenging. Data were analyzed using t-test by SPSS software. The results showed that the total haemocyte of transgenic shrimp was not significantly different (P>0.05 from non-transgenic shrimp, but haemocyte differentiation and phenoloxydase activity were significantly different (P<0.05 especially on sixth days after being exposed to the bioluminescent bacteria. The study results implied that transgenic shrimp has a better immune response compared than non-transgenic shrimp.

  9. Pembuatan Tata Kelola Keamanan Informasi Kontrol Akses Berbasis ISO/IEC 27001:2005 Pada Kantor Pelayanan Perbendaharaan Surabaya I

    Directory of Open Access Journals (Sweden)

    Margo Utomo

    2012-09-01

    Full Text Available Perkembangan teknologi informasi yang pesat saat ini turut berimbas kepada penggunaan teknologi informasi di lingkungan pemerintahan. KPPN Surabaya I sebagai instansi vertikal dari Direktorat Jenderal Perbendaharaan juga menerapkan teknologi informasi dalam untuk mendukung kegiatan pelayanan terhadap satuan kerja yang berada di lingkup bayarnya. Sayangnya masalah keamanan informasi yang merupakan bagian penting dari teknologi informasi sering kali kurang mendapatkan perhatian. Tidak dapat dipungkiri bahwa munculnya ancaman ataupun kelemahan dalam teknologi informasi dapat menggangu jalannya kegiatan pelayanan yang menggunakan teknologi informasi. Oleh karena itu diperlukan pengelolaan teknologi informasi berbasis risiko yang dituangkan dalam tata kelola untuk mengelola ancaman ataupun kelemahan yang muncul. ISO/IEC 27001:2005 merupakan framework sistem manajemen keamanan informasi yang dapat dijadikan dasar dalam pengelolaan keamanan informasi. Tata kelola keamanan informasi yang dibuat ini menitikberatkan pada kontrol akses yang merupakan salah satu kotrol keamanan dari ISO/IEC 27001:2005

  10. PERANCANGAN CASING DAN TATA LETAK KOMPONEN OZONIZER PENGAWET MAKANAN MENGGUNAKAN METODE QUALITY FUNCTION DEPLOYMENT (QFD

    Directory of Open Access Journals (Sweden)

    Zainal Fanani

    2012-02-01

    Full Text Available Penelitian ini berisi tentang perancangan casing dan tata letak komponen ozonizer pengawet makanan. Proses perancangan produk dilakukan dengan menggunakan metode Quality Function Deployment (QFD pada fase perencanaan produk (Product Planning dan fase perencanaan disain (Design Planning. Pada fase perencanaan produk, dilakukan identifikasi kebutuhan konsumen terhadap ozonizer pengawet makanan. Pengumpulan data mengenai kebutuhan konsumen diperoleh dari penyebaran angket (kuisioner kepada pengguna produk, yaitu rumah makan dan industri makanan. Setelah diperoleh daftar kebutuhan konsumen, kebutuhan-kebutuhan tersebut kemudian diolah menggunakan metode QFD pada fase perencanaan produk hingga diperoleh spestifikasi teknis produk dan House of Quality (HOQ. Spesifikasi teknis produk ini akan digunakan sebagai acuan dalam menyusun fase kedua proses QFD, yaitu fase perencanaan disain. Pada fase perencanaan disain, dihasilkan beberapa sketsa alternatif solusi. Alternatif solusi disain tersebut kemudian dievaluasi dan dilakukan penilaian untuk memilih solusi disain produk ozonizer pengawet makanan. Setelah solusi disain produk ozonizer dipilih, alternatif solusi tersebut kemudian dirancang secara detail, dan kemudian dibuat prototype produk. Prototype produk  yang dibuat adalah prototype visual, yakni gambar disain yang dianimasi untuk melihat proses perakitan, penyusunan tata letak komponen penyusun produk dan penyusunan display dan panel operasi produk. Kata Kunci   :   Kebutuhan konsumen, Quality Function Deployment, Spesifikasi Teknis Produk, Solusi Disain, Prototype Disain Produk     This research contains about casing project and ozonizer component layout of food preservative. The project is practiced using Quality Function Deployment (QFD method on product planning and design planning phase. In product planning phase, there is identification about consumer needs in ozonizer food preservative. Collecting data about consumer needs are received

  11. CMS delegation for MTB55 held at Tata Institute in Mumbai (Bombay) during CMS week 14 December 2000.

    CERN Multimedia

    A. Payn

    2000-01-01

    CMS delegation at MTB55 in Tata Institue Mumbai on 14 December 2000. From left to right Bertrand Blau/ETHZ, Stefania Farinon/INFN-Genova, Alain Hervé/CERN, Lucien Veillet/CERN, Jean-Paul Grillet/CERN, Yves Pabot/Saclay, Bruno Levesy/Saclay, Alain Payn/Saclay, François Kircher/Saclay, Benoît Curé/CERN.

  12. PROSPEK OMBUDSMAN REPUBLIK INDONESIA DALAM RANGKA MEMPERKUAT PELAKSANAAN EKSEKUSI PUTUSAN PERADILAN TATA USAHA NEGARA

    Directory of Open Access Journals (Sweden)

    Enrico Simanjuntak

    2014-07-01

    Full Text Available Posisi dan fungsi Peradilan Tata Usaha Negara (PERATUN masih belum optimal sebagaimana ditandai banyaknya putusan yang diabaikan. Mengacu kepada pendekatan sistem hukum, di mana antara PERATUN dan Ombudsman Republik Indonesia (ORI sama-sama bertujuan untuk mendorong terwujudnya good governance serta menjamin perlindungan hukum bagi masyarakat dari tindakan hukum publik administrasi pemerintahan, maka ORI sangat memungkinkan untuk diserahkan tugas dan tanggung jawab sebagai eksekutor independen putusan PERATUN. Dalam pada itu, ORI perlu diberi kewenangan untuk mendorong, mengawasi dan menjatuhkan sanksi terhadap pihak Tergugat yang tidak bersedia melaksanakan putusan PERATUN maupun terhadap pihak lain yang menghalang-halangi pelaksanaan putusan PERATUN.   The position and function of Administrative Court so weak that the verdict is much neglected. Referring to the approach of the legal system, where the Administrative Court and Ombudsmen of Republic of Indonesia (ORI equally aims to promote good governance and ensure the legal protection of the public from the actions of public administration law, the ORI is possible to be handed the duties and responsibilities as independent executor PERATUN decision. In the meantime, the Ombudsmen should be given the authority to promote, supervise and impose sanctions against the defendant who is not willing to implement the decision PERATUN or against other parties who obstruct the implementation of the decision PERATUN.

  13. Operation of Cryogenic Facility in e-way at Tata Institute of Fundamental Research, Mumbai, India

    International Nuclear Information System (INIS)

    Srinivasan, K V

    2012-01-01

    In an attempt towards the development of modern, model and paperless cryogenic facility, the Low Temperature Facility of Tata Institute of Fundamental Research, at Mumbai, India; carried out many automation works using programmable logic controller (PLC) and other modern electronic tools, with the objective of bringing the entire plant operation to your palm whenever and wherever you are. Efficiency in the plant operation by keeping a watch on the plant healthiness, advance indication about the possible plant problem by means of pre-warning alarms, so that the remedial action can be taken well prior to the actual failure affects the plant operation, reduction in plant down time were achieved by the automation works. Large size in our cryogen production, controlling the complicated helium liquefier, meeting the uninterrupted supply of cryogen to the users on “any time availability basis,” safety in handling cryogens and high pressure gas, effective usage of limited skilled manpower etc., all these requirements call for the definite need of modern electronic gears and gadgets. This paper will describe in details about the automation works carried out at our cryogenic facility at TIFR.

  14. PENGEMBANGAN BUKU AJAR MATA PELAJARAN TATA HIDANG PAKET KEAHLIAN JASA BOGA SEKOLAH MENENGAH KEJURUAN

    Directory of Open Access Journals (Sweden)

    Yuli Astuti

    2016-01-01

    Full Text Available Textbooks are the ingredients or the subject matter is arranged systematically used by students in the learning process. The purpose of this study was to develop teaching materials such as textbooks on the subjects of governance hidang class X SMK in Magetan consisting of 3 KD, namely, (1 The scope of food and beverage service, (2 Operating equipment food and beverage service, and (3 providing food and beverage service at the restaurant. The results show that the development of an overall assessment of the completeness of the presentation of the textbook of the development of criteria that are in "good", which means it does not need to be revised textbooks. Buku ajar adalah bahan-bahan atau materi pelajaran yang disusun secara sistematis yang digunakan oleh siswa dalam proses belajar mengajar. Tujuan dari penelitian ini adalah untuk mengembangkan bahan ajar berupa buku ajar pada mata pelajaran tata hidang kelas X SMK di Kabupaten Magetan yang terdiri dari 3 KD yaitu, (1 Ruang lingkup pelayanan makanan dan minuman, (2 Mengoperasikan peralatan layanan makanan dan minuman, dan (3 menyediakan layanan makanan dan minuman di restoran. Hasil pengembangan menunjukkan bahwa secara keseluruhan penilaian tentang kelengkapan penyajian terhadap buku ajar hasil pengembangan berada pada kriteria “baik”, yang berarti buku ajar tidak perlu direvisi.

  15. PENGUNGKAPAN RISIKO FINANSIAL DAN TATA KELOLA PERUSAHAAN: STUDI EMPIRIS PERBANKAN INDONESIA

    Directory of Open Access Journals (Sweden)

    Djoko Suhardjanto

    2017-03-01

    Full Text Available The purpose of this study was to examine the effect of corporate governance to financial risk disclosures ofIndonesian banks. Corporate governance was identified as the board size, the number of board meetings, theproportion of independent commissioners, the proportion of independent audit committee members and numberof audit committee meetings. This study also used leverage and profitability as control variable. The levelof financial risk disclosure was measured based on identified items of a circular enclosure of Bank IndonesiaNo.5/21/DPNP/2003. Under purposive sampling, secondary data of 60 annual reports year 2007-2009 ofbanks in Indonesian Stock Exchange were selected. The average level of financial risk disclosures was at46.50%. It indicated that Indonesian’s banks were not fully compliance to regulations since financial riskdisclosures were as mandatory matters. The result of multiple regression showed that corporate governanceaffected the level of financial risk disclosure through the variable board size and the number of board meetings.The important role in implementing tata kelola perusahaan(company management was at the board of commissionerswho served as supervisors of activities and performance of banks as well as advisory directors inensuring that companies implemented good corporate governance, including financial risk disclosures (KomiteNasional Kebijakan Governance, 2006.

  16. Setting up a Tissue Bank in India: The Tata Memorial Hospital Experience.

    Science.gov (United States)

    Gajiwala, A L

    2003-01-01

    In India, the procurement of tissues for transplantation is governed by the Transplantation of Human Organs Act, 1994. However, although this law exists, it is primarily applied to organ transplantation and rules and regulations that are specific to tissue banking have yet to be developed.The Tata Memorial Hospital (TMH) Tissue Bank was started in 1988 as part of an International Atomic Energy Agency (IAEA) programme to promote the use of ionising radiation for the sterilisation of biological tissues. It represents the Government of India within this project and was the first such facility in the country. It is registered with the Health Services Maharashtra State and provides lyophilised amnion, dura mater, skin and bone that have been terminally sterilised with exposure to 25 kGy of gamma radiation from a Cobalt 60 source. These are obtained either from cadavers or live donors.To date the TMH Tissue Bank has provided 6328 allografts for use as biological dressings or in various reconstructive procedures.The TMH Tissue Bank has helped initiate a Tissue Bank at the Defence Laboratory (DL), Jodhpur. At present these are the only two Banks in the country using radiation for terminal sterilisation of banked tissues.The availability of safe, clinically useful and cost effective grafts have resulted in changes in surgical treatment with a concomitant increase in demand for grafts and an interest in developing more tissue banks. The availability of donor tissue however, continues to be a major limitation.

  17. Evaluasi penggunaan tepung cangkang rajungan sebagai bahan baku pakan juwana udang windu Penaeus monodon

    Directory of Open Access Journals (Sweden)

    Agus Kurnia

    2016-12-01

    Full Text Available ABSTRACT Feeding trial was conducted to determine the optimum dietary level of the crab shell meal (CSM for replacement of fish meal (FM for growth and survival rate of black tiger shrimp larvae. The shrimp (initial weight: 0,0134±0,02 g were fed with six experimental diet for six weeks which were formulated to replace FM protein by with CSM at various substitution levels: diet A (0% CSM substitution level, diet B (25% CSM substitution level, diet C (50% CSM substitution level, diet D (75% CSM substitution level, diet E (100% CSM substitution level, diet F (commercial diet. Results from the feeding trial indicates that the shrimp fed with all treatment diet were not significantly different in weight gain and FCR. However, survival rate on the shrimp fed with diet F was significantly different to the other groups. The present study conclude that CSM could be used as protein ingredient in the diet of monodon shrimp juvenile. Keywords: replacement, crabs shell meal, fish meal, black tiger shrimp, survival rate  ABSTRAK Penelitian pakan dilakukan untuk menentukan dosis optimum tepung cangkang rajungan (TCR untuk mengganti tepung ikan (TI dalam pakan terhadap pertumbuhan dan kelangsungan hidup larva udang windu. Udang windu (berat awal: 0,00134±0,02 g diberi pakan uji selama enam minggu yang diformulasi untuk mengganti TI dengan tingkat persentasi penggantian TCR dengan desain formulasi pakan A (0% substitusi TCR, pakan B (25% substitusi TCR, pakan C (50% substitusi TCR, pakan D (75% substitusi TCR, pakan E (100% substitusi TCR, dan pakan F (pakan komersial sebagai pakan kontrol. Hasil dari penelitian ini menunjukkan bahwa udang yang diberi pakan untuk semua perlakuan tidak memberikan pengaruh nyata terhadap pertumbuhan dan laju pertumbuhan harian. Akan tetapi kelompok udang yang diberi pakan F berpengaruh nyata terhadap kelangsungan hidup dibanding dengan perlakuan lainnya. Hasil penelitian ini menyimpulkan bahwa tepung cangkang rajungan

  18. Copper accumulation and transport in a marine food chain composed of Platymonas subcordiformis, brachionus plicatilis and Penaeus monodon

    Science.gov (United States)

    Cai, A.-Gen; Chen, Wei-Qi; Li, Wen-Quan

    1997-09-01

    Accumulation, transport and toxicity of Cu in the food chain consisting of Platymonas subcordiformis, Brachionus plicatilis and Penaeus monodon were studied. Effects of Cu on the growth of organisms in the food chain were investigated and the inhibiting effect concentration (EC50) of Cu was then determined according to the dynamics of the relative number of cells or total individuals of organisms, expressed in percentages with reference to the controlled system, under different culture conditions. On the basis of the variations in accumulation and percentages of accumulation of Cu in the biological phase, the relationship between the accumulation of Cu in organisms and its toxicity was analyzed and the main approach for determining the transport of Cu in the food chain was then discussed.

  19. Effect of dietary pigment on growth performance and disease resistance in black tiger shrimp post larva (Penaeus monodon, Fabricius

    Directory of Open Access Journals (Sweden)

    Boonyaratpalin, M.

    2005-02-01

    Full Text Available Effects of dietary pigment on survival and disease (white spot syndrome virus: WSSV resistance in black tiger shrimp post larva (Penaeus monodon, Fabricius (PL15 for a 30-day period were studied. The results showed that not only was mean survival of black tiger shrimp (PL15 fed with supplementation of Lucarotene or Betatene at 125 mg/kg diet significantly higher (P<0.05 but also the body color was increased. There were no effects of dietary pigment on mean weight, percent weight gain and WSSV resistance. However, mean WSSV resistance of black tiger shrimp (PL15 fed diet containing Lucantin pink 50 mg/kg diet, Spirulina 30 g/kg diet or Betatene 125 mg/kg diet was higher than that of control.

  20. Poly-β-hydroxybutyrate (PHB) accumulating Bacillus spp. improve the survival, growth and robustness of Penaeus monodon (Fabricius, 1798) postlarvae.

    Science.gov (United States)

    Laranja, Joseph Leopoldo Q; Ludevese-Pascual, Gladys L; Amar, Edgar C; Sorgeloos, Patrick; Bossier, Peter; De Schryver, Peter

    2014-10-10

    Low larval survival resulting from suboptimal culture conditions and luminous vibriosis poses a major problem for the larviculture of penaeid shrimp. In this study, a poly-β-hydroxybutyrate (PHB) accumulating mixed bacterial culture (mBC; 48.5% PHB on cell dry weight) and two PHB accumulating bacterial isolates, Bacillus sp. JL47 (54.7% PHB on cell dry weight) and Bacillus sp. JL1 (45.5% PHB on cell dry weight), were obtained from a Philippine shrimp culture pond and investigated for their capacity to improve growth, survival and robustness of Penaeus monodon postlarvae (PL). Shrimp PL1 and shrimp PL30 were provided with the PHB containing bacterial cultures in the feed for 30 days followed by, respectively, a challenge with pathogenic Vibrio campbellii and exposure to a lethal dose of ammonia. Prior to the pathogenic challenge or ammonia stress, growth and survival were higher for shrimp receiving the PHB accumulating bacteria as compared to shrimp receiving diets without bacterial additions. After exposure to the pathogenic challenge the shrimp fed PHB accumulating bacteria showed a higher survival as compared to non-treated shrimp, suggesting an increase in robustness for the shrimp. Similar effects were observed when shrimp PL30 were provided with the PHB accumulating bacterial cultures during a challenge with pathogenic V. campbellii through the water. The survival of shrimp exposed to lethal ammonia stress showed no significant difference between PHB accumulating bacteria-fed shrimp and non-PHB treated shrimp. The data illustrate that bacilli capable of accumulating PHB can provide beneficial effects to P. monodon post-larvae during culture in terms of growth performance, survival and resistance against pathogenic infection and ammonia stress. Further investigations are required to verify the PHB effect of the bacterial cultures on the shrimp. Copyright © 2014 Elsevier B.V. All rights reserved.

  1. Effect of Adding the White Legged Shrimp (Penaeus vannamei on Growth and Survival of Tiger Shrimp (Penaeus monodon in Intensive Farming

    Directory of Open Access Journals (Sweden)

    . Tarsim

    2007-12-01

    Full Text Available This experiment was carried out in the framework of a project to develop a viable shrimp polyculture technology under intensive farming. The objectives were to assess the effect of adding white legged shrimp (Penaeus vannamei on growth and survival of tiger shrimp (Penaeus monodon in intensive Farming. Tiger shrimp were stocked in 2800-3400 m2 earthen ponds at 40 individuals/m2 and reared for 133 days. White legged shrimp added at 70 day at 8±2 individuals/m2. Growth rate and total yield affected by addition of this species. Final  affected by white legged shrimp addition. No adding effects were found on the water quality parameters. The results indicate that, at densities tested, white legged shrimp addition to the intensive farming of tiger shrimp polyculture is viable as species increase crop production Key words: Penaeus vannamei, P. monodon, growth, survival, polyculture

  2. A cDNA microarray, UniShrimpChip, for identification of genes relevant to testicular development in the black tiger shrimp (Penaeus monodon

    Directory of Open Access Journals (Sweden)

    Klinbunga Sirawut

    2011-04-01

    Full Text Available Abstract Background Poor reproductive maturation in captive male broodstock of the black tiger shrimp (Penaeus monodon is one of the serious problems to the farming industries. Without genome sequence, EST libraries of P. monodon were previously constructed to identify transcripts with important biological functions. In this study, a new version of cDNA microarray, UniShrimpChip, was constructed from the Peneaus monodon EST libraries of 12 tissues, containing 5,568 non-redundant cDNA clones from 10,536 unique cDNA in the P. monodon EST database. UniShrimpChip was used to study testicular development by comparing gene expression levels of wild brooders from the West and East coasts of Thailand and domesticated brooders with different ages (10-, 14-, 18-month-old. Results The overall gene expression patterns from the microarray experiments revealed distinct transcriptomic patterns between the wild and domesticated groups. Moreover, differentially expressed genes from the microarray comparisons were identified, and the expression patterns of eight selected transcripts were subsequently confirmed by reverse-transcriptase quantitative PCR (RT-qPCR. Among these, expression levels of six subunits (CSN2, 4, 5, 6, 7a, and 8 of the COP9 signalosome (CSN gene family in wild and different ages of domesticated brooders were examined by RT-qPCR. Among the six subunits, CSN5 and CSN6 were most highly expressed in wild brooders and least expressed in the 18-month-old domesticated group; therefore, their full-length cDNA sequences were characterized. Conclusions This study is the first report to employ cDNA microarray to study testicular development in the black tiger shrimp. We show that there are obvious differences between the wild and domesticated shrimp at the transcriptomic level. Furthermore, our study is the first to investigate the feasibility that the CSN gene family might have involved in reproduction and development of this economically important

  3. MARINE SPONGE Aaptos suberitoides, IT’S POTENTIAL SOURCE OF NATURAL ANTIBACTERIAL FOR CONTROLLING Vibrio harveyi ON TIGER SHRIMP (Penaeus monodon) CULTURE

    OpenAIRE

    Rosmiati Rosmiati; Andi Parenrengi; Emma Suryati

    2015-01-01

    The study aims to isolate and identify the natural antibacterial compounds potential from Aaptos suberitoides for Vibrio harveyi control on tiger shrimp (Penaeus monodon) culture. The agar diffusion method using paper discs was used to determine the antibacterial activity of extracts (diethyl ether (DEE), butanol (BUE) and aqueous (HOE) and compounds successfully isolated against Vibrio harveyi. Findings showed that the antibacterial activity was concentrated in BUE with the inhibition zone o...

  4. EVALUASI PENERAPAN ICT DALAM MENDUKUNG KETERAMPILAN SAINTIFIK PADA PEMBELAJARAN TATA SURYA

    Directory of Open Access Journals (Sweden)

    Septiana Indri Hapsari

    2017-02-01

    Full Text Available Abstrak ___________________________________________________________________ Tujuan penelitian ini untuk mengetahui persentase penerapan ICT dalam mendukung keterampilan saintifik pada pembelajaran IPA tema tata surya. Pemilihan sampel dengan cara purpossive sampling, diperoleh guru IPA dan peserta didik kelas VIII di SMPN 5, 8, 15 Yogyakarta dan SMP Pangudi Luhur 1 Yogyakarta. Penelitian ini merupakan penelitian Evaluasi dengan model yang digunakan adalah Countenance Stake. Instrumen yang digunakan adalah angket peserta didik, angket guru, serta observasi. Data diolah dengan menggunakan deskriptif.  Hasil penelitian ini adalah 77,54% peserta didik mengaku ICT membantu kegiatan mengamati, 57,97% ICT membantu kegiatan menanya, 75,36% ICT membantu kegiatan mengumpulkan informasi, 57,00% ICT membantu kegiatan mengasosiasi, dan 42,75% ICT membantu kegiatan mengkomunikasikan. Dengan demikian penerapan ICT cukup mendukung keterampilan saintifik, namun harus tetap perlu ditingkatkan. Abstract ___________________________________________________________________ The purpose of this study to determine the percentage of the application of ICT in supporting scientific skills in learning science theme of the solar system. Election sample by purposive sampling, obtained a science teacher and students in class VIII SMPN 5, 8, 15 and SMP Pangudi Luhur Yogyakarta 1 Yogyakarta. This study is evaluation of the model used is the Countenance Stake. The instrument used was a questionnaire the learner, teacher questionnaire, and observation. The data was processed using descriptive. The results of this study are 77,54% of students admitted to ICT helps the activity observed, 57,97% ICT help activities ask, 75,36% ICT help in gathering information, 57,00% ICT assist the associates, and 42,75% ICT help communicate the activities. Thus the application of ICT support enough scientific skills, but must still be improved.

  5. POTENSI PERKEMBANGAN WILAYAH DAN KAITANNYA DENGAN TATA RUANG DI KAWASAN LERENG MERAPI PROPINSI DAERAH ISTIMEWA YOGYAKARTA

    Directory of Open Access Journals (Sweden)

    Luthfi Muta'ali

    2016-10-01

    Full Text Available ABSTRAK Penelitian ini bertujuan untuk mengkaji potensi dan perkembangan wilayah kawasan lereng Merapi yang dikalikan dengan peruntukan fungsi tata ruang. Lingkup wilayah dan unit analisis adalah seluruh desa di kawasan pengembangan Lereng Merapi, yaitu sejumlah 206 desa yang tersebar di Kabupaten Sleman, Kota Yogyakarta, dan Kabupaten Banta Penelitian menggunakan pendekatan deskriptif analitis, dengan menggunakan data sekunder. Potensi perkembangan wilayah diidentifikasi dengan indikator demografis, karakteristik social ekonomi, penggunaan lahan dan infrastruktur, dan aksesibilitas. Sedangkan data peruntukan ruang dikelompokkan dalam peruntukan fungsi kawasan lindung dan kawasan budidaya. Teknik analisis yang digunakan antara lain stalistik deskriptip, penentuan tipologi wilayah, analisis deskriminan, shift analysis, dan pemetaan. Hasil penelitian menunjukkan, pola perkembangan wilayah di kawasan lereng Merapi terkonsentrasi di bagian tengah (kota Yogyakarta dan pinggirannya dan menuju kearah lereng atas (Kabupaten Sleman. Pada fungsi budidaya, sebagian besar wilayah bertipe 1: (besar tumbuh berada di daerah perkotaan dan perluasannya, sedangkan wilayah ripe 1l (kecil tumbuh urnumnya berfungsi sebagai daerah pertanian lahan basah. Pada peruntukan fungsi lindung, khususnya lindung bawahan (resapan air, terdapat 36,7% (18 desa yang berpotensi berkembang pesat. Analisis basis ekonomi juga menunjukkan bahwa potensi perkembangan wilayah tinggi, akan diiringi oleh pergeseran menguatnya peran sektor non pertanian. Tipologi wilayah menurut fungsi kawasan dapat digunakan sebagai dasar dalam determinasi perkembangan wilayah, karena memiliki tingkat perbedaan yang signifikan antara fungsi lindung dan budidaya. Gejala potensi perkembangan wilayah yang tinggi di lereng bagian tengah dan atas yang berfungsi sebagai kawasan lindung dan resapan tidak menguntungkan dart sisi ekologis, dapat mengakibatkan kerusakan lingkungan. Oleh karena itu penelitian ini

  6. Penilaian Tingkat Kemapanan Tata Kelola Teknologi Informasi Berbasis COBIT 4.1 Pada Layanan Portal Akademik Online (Studi Kasus : UIN Maulana Malik Ibrahim Malang

    Directory of Open Access Journals (Sweden)

    Fachrul Kurniawan

    2014-09-01

    Full Text Available Pemanfaatan TI dalam dunia pendidikan merupakan hal yang sangat penting untuk menunjang kegiatan akademiknya. Termasuk juga di Universitas Islam Negeri Maulana Malik Ibrahim Malang yang terkenal dengan sistem administrasi akademik online atau biasa disebut siakad. Namun dalam pemanfatan tersebut belum bisa diketahui sejauh mana TI yang digunakan bisa mendukung proses bisnis yang ada di universitas, khususnya yang terkait dengan layanan akademik online. Oleh karena itu perlu adanya penilaian resmi dan prosedural terkait kualitas tata kelola TI pada layanan portal akademik online. Proses penilaian tata kelola TI ini menggunakan framework COBIT 4.1. Penelitian ini dilakukan di Lembaga Pengembangan Sistem Informasi (LPSI. Dalam proses penilaian tata kelola TI tersebut COBIT 4.1 menggunakan sistem pemetaan proses bisnis sehingga terpilih proses TI terkait COBIT atau dengan diagram value chain. Hasil dari pemetaan proses bisnis berdasarkan COBIT 4.1 terpilih proses TI DS1 Define and manage service levels dan DS4 Ensure continuous service sebagai acuan penilaian dengan hasil perhitungan tingkat kemapanan kedua proses TI pada level 3 (Defined. Namun LPSI memiliki keinginan dan harapan besar dalam meningkatkan tingkat kemapanan TI yang semakin baik, yaitu tata kelola yang optimal. Hal ini ditunjukkan dari hasil perhitungan tingkat kemapanan yang diharapkan (to-be , yaitu berada pada level 5 (Optimised. Kata kunci : tingkat kemapanan, tata kelola ti, COBIT, portal akademik online.

  7. Optimizations of siRNA design for the activation of gene transcription by targeting the TATA-box motif.

    Directory of Open Access Journals (Sweden)

    Miaomiao Fan

    Full Text Available Small interfering RNAs (siRNAs are widely used to repress gene expression by targeting mRNAs. Some reports reveal that siRNAs can also activate or inhibit gene expression through targeting the gene promoters. Our group has found that microRNAs (miRNAs could activate gene transcription via interaction with the TATA-box motif in gene promoters. To investigate whether siRNA targeting the same region could upregulate the promoter activity, we test the activating efficiency of siRNAs targeting the TATA-box motif of 16 genes and perform a systematic analysis to identify the common features of the functional siRNAs for effective activation of gene promoters. Further, we try various modifications to improve the activating efficiency of siRNAs and find that it is quite useful to design the promoter-targeting activating siRNA by following several rules such as (a complementary to the TATA-box-centered region; (b UA usage at the first two bases of the antisense strand; (c twenty-three nucleotides (nts in length; (d 2'-O-Methyl (2'-OMe modification at the 3' terminus of the antisense strand; (e avoiding mismatches at the 3' end of the antisense strand. The optimized activating siRNAs potently enhance the expression of interleukin-2 (IL-2 gene in human and mouse primary CD4+ T cells with a long-time effect. Taken together, our study provides a guideline for rational design the promoter-targeting siRNA to sequence-specifically enhance gene expression.

  8. International Conference on Analytic and Algebraic Geometry held at the Tata Institute of Fundamental Research and the University of Hyderabad

    CERN Document Server

    Biswas, Indranil; Morye, Archana; Parameswaran, A

    2017-01-01

    This volume is an outcome of the International conference held in Tata Institute of Fundamental Research and the University of Hyderabad. There are fifteen articles in this volume. The main purpose of the articles is to introduce recent and advanced techniques in the area of analytic and algebraic geometry. This volume attempts to give recent developments in the area to target mainly young researchers who are new to this area. Also, some research articles have been added to give examples of how to use these techniques to prove new results.

  9. Dinamika Tata Kelola Kebijakan Industri Penyiaran dan Telekomunikasi Indonesia Menuju Konvergensi

    Directory of Open Access Journals (Sweden)

    Vience Mutiara Rumata

    2016-03-01

    Full Text Available Abstrak Konvergensi antara penyiaran dan telekomunikasi di Indonesia tidak terhindarkan seiring dengan perkembangan teknologi informasi dan komunikasi (TIK serta ketatnya persaingan dalam industri keduanya. Kedua industri telah mengintegrasi TIK sebagai nilai tambah dari layanan ataupun produk yang ditawarkannya. Meski demikian, tata kelola kedua industri ini masih terpisah yakni melalui UU Penyiaran tahun 2002 dan UU Telekomunikasi tahun 1999. Di saat RUU konvergensi telematika belum menemukan titik terang, regulator berupaya merevisi kedua UU tersebut. Studi ini bertujuan untuk memahami relasi kekuasaan serta pandangan para pemangku kebijakan dalam mengatur industri penyiaran dan telekomunikasi di era konvergensi. Teknik pengumpulan data dilakukan dengan cara wawancara mendalam kepada narasumber yang dipilih secara purposif yakni Kemkominfo, KPI, BRTI, dan DPR, selama Januari-Februari 2015. Setiap institusi memiliki path dependence yang berbeda antar satu dengan yang lain, sehingga membentuk pola distribusi kekuasan berbeda dalam prosedur pembuatan kebijakan. Proposal revisi UU baik dari Kemkominfo dan KPI tidak mengatur hal-hal yang berkaitan dengan konvergensi. Akan tetapi, perebutan otoritas antar kedua institusi tersebut justru menjadi fokus dalam proposal tersebut. Kemkominfo, sebagai inisiator UU Telekomunikasi, berencana untuk mengubah revisi UU tersebut menjadi UU konvergensi.     Abstract The convergence between broadcasting and telecommunication in Indonesia is inevitable as the growing information and communication technology and competition within both industries. Nevertheless, the policy governance of these industries remains distinctively separated under the 2002 Broadcasting Law and the 1999 Telecommunication Law. Whilst the regulators failed to enact the convergence bill, the only way is to synergize the revision of both laws.. This study aims to understand the point of views as well as power relation among regulators in

  10. PENGEMBANGAN BAHAN AJAR POLA DASAR BUSANA WANITA PADA PROGRAM STUDI D3 TATA BUSANA

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    Idah Hadijah

    2014-05-01

    Full Text Available Teaching Materials Development of the Basic Pattern of Women's Clothing on Clothing Design Program. The purpose of this development is to pro­­duce teaching materials of archetypal body of woman fashion, whichis equipped with a Power Po­int in order to assist faculty and students on conducting learning ac­ti­vi­ty. The research development me­thod used was adapted from the instructional de­sign model of Dick and Carey. The results of the study in the form of assess­ment/feedback showed that the mean score reached 86.00%, which meant that the teaching materials categorized as well qualify­ed/ decent, the media has a very good Power Point as it re­ached 91.00%, the lecturers gui­de reached 92.00% and catehorized as ex­cell­ent/ve­ry decent, and the students guide achieved 83.00% and categorized as good/de­cent. Thus, the teaching material is feasible to be used as the instructional materials for learning on Clothing Design Program.   Tujuan pengembangan ini un­­­tuk menghasilkan produk bahan ajar po­­la dasar ba­dan busana wanita dilengkapi de­ngan Power Point, sehingga dapat membantu dosen dan mahasiswa dalam me­lak­sa­na­kan pembelajaran. Metode penelitian pengembangan yang digunakan diadaptasi dari ran­­­cangan pembelajaran model Dick dan Carey. Hasil pe­nelitian berupa penilaian/tanggapan menunjukan rerata skor bah­­wa bahan ajar mencapai 86,00% termasuk ke da­lam kualifikasi baik/layak, media Po­­wer Point termasuk sangat baik karena men­capai 91,00%, pan­­duan dosen mencapai 92,00% termasuk ke dalam kualifikasi sangat ba­ik/sa­­­­ngat layak, dan panduan ma­ha­siswa mencapai 83,00% termasuk kua­lifikasi ba­ik/la­yak, dengan demikian bahan ajar ter­­sebut layak digunakan untuk pem­­b­e­lajaran pada Program Studi D3Tata Busana.

  11. TAF(II)170 interacts with the concave surface of TATA-binding protein to inhibit its DNA binding activity.

    Science.gov (United States)

    Pereira, L A; van der Knaap, J A; van den Boom, V; van den Heuvel, F A; Timmers, H T

    2001-11-01

    The human RNA polymerase II transcription factor B-TFIID consists of TATA-binding protein (TBP) and the TBP-associated factor (TAF) TAF(II)170 and can rapidly redistribute over promoter DNA. Here we report the identification of human TBP-binding regions in human TAF(II)170. We have defined the TBP interaction domain of TAF(II)170 within three amino-terminal regions: residues 2 to 137, 290 to 381, and 380 to 460. Each region contains a pair of Huntington-elongation-A subunit-Tor repeats and exhibits species-specific interactions with TBP family members. Remarkably, the altered-specificity TBP mutant (TBP(AS)) containing a triple mutation in the concave surface is defective for binding the TAF(II)170 amino-terminal region of residues 1 to 504. Furthermore, within this region the TAF(II)170 residues 290 to 381 can inhibit the interaction between Drosophila TAF(II)230 (residues 2 to 81) and TBP through competition for the concave surface of TBP. Biochemical analyses of TBP binding to the TATA box indicated that TAF(II)170 region 290-381 inhibits TBP-DNA complex formation. Importantly, the TBP(AS) mutant is less sensitive to TAF(II)170 inhibition. Collectively, our results support a mechanism in which TAF(II)170 induces high-mobility DNA binding by TBP through reversible interactions with its concave DNA binding surface.

  12. PERANCANGAN TATA LETAK DAN FASILITAS HUNIAN RUKOST BERDASARKAN KEBUTUHAN KONSUMEN UNTUK MENINGKATKAN PANGSA PASAR DENGAN MENGGUNAKAN ASPEK ERGONOMI

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    Leola Dewiyani

    2018-04-01

    Full Text Available Saat ini Karawang dikenal sebagai daerah industri yang paling berpotensi di wilayah Jawa Barat. Hal tersebut dibuktikan dengan banyaknya pabrik yang didirikan di tanah Pasundan ini. Dengan banyaknya usaha baik berupa pabrik maupun yang lainnya, Karawang menjanjikan aneka macam peluang di berbagai bidang usaha yang bisa mendatangkan keuntungan. Perusahaan ini tentu membutuhkan tenaga kerja yang cukup banyak, tidak hanya buruh lebih dari itu tenaga kerja yang dibutuhkan juga berasal dari level menengah ke atas hingga staf profesional, hal ini yang menjadi daya tarik bagi pengembang untuk membangun bisnis properti di Karawang yaitu RuKost (rumah kos. Akan tetapi Tata Letak interior kamar yang ada di RuKost saat ini berdasarkan penelitian pendahuluan yang dilakukan peneliti belum memenuhi standar dan juga belum memperhatikan kenyamanan, kepuasan, keselamatan dan kesehatan karena desain layout interior yang ada pada kamar RuKost saat ini kurang memperhatikan prinsip-prinsip ergonomik. Berdasarkan kondisi di atas maka akan dilakukan suatu redesign terhadap tata letak interior kamar RuKost. Metode perancangan yang akan digunakan dalam penelitian ini adalah antropometri dan physiological performance.

  13. Penapisan Bakteri yang Diisolasi dari Tambak Udang sebagai Kandidat Probiotik pada Budi daya Udang Windu, Penaeus monodon

    Directory of Open Access Journals (Sweden)

    Muliani Muliani

    2016-11-01

    Full Text Available Penelitian ini bertujuan mendapatkan bakteri probiotik untuk budi daya udang windu P. monodon. Penelitian meliputi beberapa tahapan yaitu (1 isolasi bakteri dari tambak udang; (2 uji daya hambat terhadap Vibrio  harveyi; (3 karakterisasi secara fisiologi dan biokimia; (4 pertumbuhan bakteri pada beberapa konsentrasi NaCl; (5 pertumbuhan bakteri pada beberapa tingkat salinitas; (6 uji patogenisitas bakteri terhadap pascalarva udang windu; (7 uji tantang dengan V. harveyi dalam wadah pemeliharaan pascalarva udang windu; dan (8 analisis gen 16S-rRNA bakteri yang diisolasi dari tambak. Sedikitnya 14 isolat dari 2.228 isolat bakteri yang diisolasi dari tambak, potensial dijadikan probiotik pada budi daya udang windu. Sintasan udang windu tertinggi pada perlakuan yang menggunakan isolat BN2067. Isolat BT950 dan BT95 paling potensial menghambat pertumbuhan V. harveyi baik secara In vitro maupun In vivo. Hasil analisis gen 16Sr-RNA menunjukkan bahwa BT950 dan BT951 termasuk dalam kelompok Brevibacillus sp., sedangkan BN2067 termasuk dalam kelompok Vibrio vulnificus CMCP6 chr. This experiment was aimed for finding-out probiotic bacteria on tiger shrimp P. monodon culture. The research included several steps i.e. 1 isolation of bacteria from tiger shrimp pond; 2 inhibition test of  bacteria against V. harveyi; 3 biochemical and physiological characterization; 4 growth of bacteria at different concentration of NaCl; (5 growth of bacteria at different salinities; (6 pathogenicity test of bacteria to tiger shrimp post larvae, (7 challenge test of bacteria against V. harveyi in tiger shrimp culture media; (8 16S-rRNA gene analysis of  bacteria isolated from shrimp pond. Fourteen isolates of 2,228 isolates of bacteria isolated from tiger shrimp pond were potential for probiotic bacteria on tiger shrimp culture. The highest survival rate of tiger shrimp was obtained from those treated with BN2067 isolate. The potential isolates to inhibit V. harveyi both In

  14. Improving the quality of tiger shrimp Penaeus monodon through dietary incorporation of algae as a source of natural pigment

    Directory of Open Access Journals (Sweden)

    Kunal Mondal

    2015-01-01

    Full Text Available Tiger shrimp is one of the major candidate species for export oriented aquaculture which dominates the seafood market in regions of European Union, Japan and USA. Carotenoid content in seafood has now become one of the important criteria in determining the quality of edible product. Recent trends in supplementing fish diets with natural pigment source are an alternative to the utilization of expensive synthetic pigments. In this context, green algae Enteromorpha intestinalis was selected as a source of natural pigment for inclusion in the diet of tiger shrimp Penaeus monodon. Astaxanthin being an important category of carotenoid pigment was monitored in shrimp muscle tissue during the feeding trial. Significant variation (p<0.05 was observed between the experimental groups as confirmed through ANOVA thus exhibiting higher astaxanthin content of shrimps (18.70 Å} 4.48 ppm fed with E. intestinalis incorporated diet as compared to control (15.80 Å} 2.33 ppm. The present programme therefore emphasizes on the quality improvement of aquaculture product by dietary inclusion of algae as a natural pigment source.

  15. Inhibition of luminescence and virulence in the black tiger prawn (Penaeus monodon) pathogen Vibrio harveyi by intercellular signal antagonists.

    Science.gov (United States)

    Manefield, M; Harris, L; Rice, S A; de Nys, R; Kjelleberg, S

    2000-05-01

    Expression of luminescence in the Penaeus monodon pathogen Vibrio harveyi is regulated by an intercellular quorum sensing mechanism involving the synthesis and detection of two signaling molecules, one of which is N-hydroxy butanoyl-L-homoserine lactone and the other of which is uncharacterized. Indirect evidence has suggested that virulence, associated with a toxic extracellular protein, and luminescence in V. harveyi are coregulated. In this study the effects of an acylated homoserine lactone antagonist produced by the marine alga Delisea pulchra on luminescence and toxin production in a virulent strain of V. harveyi were analyzed. Luminescence and toxin production were both inhibited by the signal antagonist at concentrations that had no impact on growth. Toxin production was found to be prematurely induced in V. harveyi cultures incubated in a 10% conditioned medium. Additionally, a significant reduction in the toxicity of concentrated supernatant extracts from V. harveyi cultures incubated in the presence of the signal antagonist, as measured by in vivo toxicity assays in mice and prawns, was observed. These results suggest that intercellular signaling antagonists have potential utility in the control of V. harveyi prawn infections.

  16. Inhibition of Luminescence and Virulence in the Black Tiger Prawn (Penaeus monodon) Pathogen Vibrio harveyi by Intercellular Signal Antagonists

    Science.gov (United States)

    Manefield, Michael; Harris, Lachlan; Rice, Scott A.; de Nys, Rocky; Kjelleberg, Staffan

    2000-01-01

    Expression of luminescence in the Penaeus monodon pathogen Vibrio harveyi is regulated by an intercellular quorum sensing mechanism involving the synthesis and detection of two signaling molecules, one of which is N-hydroxy butanoyl-l-homoserine lactone and the other of which is uncharacterized. Indirect evidence has suggested that virulence, associated with a toxic extracellular protein, and luminescence in V. harveyi are coregulated. In this study the effects of an acylated homoserine lactone antagonist produced by the marine alga Delisea pulchra on luminescence and toxin production in a virulent strain of V. harveyi were analyzed. Luminescence and toxin production were both inhibited by the signal antagonist at concentrations that had no impact on growth. Toxin production was found to be prematurely induced in V. harveyi cultures incubated in a 10% conditioned medium. Additionally, a significant reduction in the toxicity of concentrated supernatant extracts from V. harveyi cultures incubated in the presence of the signal antagonist, as measured by in vivo toxicity assays in mice and prawns, was observed. These results suggest that intercellular signaling antagonists have potential utility in the control of V. harveyi prawn infections. PMID:10788385

  17. A probiotic Bacillus strain containing amorphous poly-beta-hydroxybutyrate (PHB) stimulates the innate immune response of Penaeus monodon postlarvae.

    Science.gov (United States)

    Laranja, Joseph Leopoldo Q; Amar, Edgar C; Ludevese-Pascual, Gladys L; Niu, Yufeng; Geaga, Mary Joy; De Schryver, Peter; Bossier, Peter

    2017-09-01

    In this study, the PHB-accumulating Bacillus sp. JL47 strain (capable of accumulating 55% PHB on cell dry weight) was investigated for its effects on the immune response of giant tiger shrimp (Penaeus monodon) postlarvae (PL) before and after the Vibrio campbellii challenge. Briefly, shrimp PL were cultured and fed with Artemia nauplii enriched with Bacillus sp. JL47. Shrimp receiving the Artemia nauplii without JL47 enrichment were used as control. After 15 days of feeding, the shrimp were challenged with pathogenic V. campbellii LMG 21363 at 10 6  cells mL -1 by immersion. Relative expression of the immune related genes encoding for prophenoloxidase (proPO), transglutaminase (TGase) and heat shock protein 70 (Hsp70) in the shrimp were measured before (0 h) and after (3, 6, 9, 12, 24 h) the Vibrio challenge by quantitative real-time PCR using β-actin as the reference gene. The expressions of TGase and proPO were significantly up-regulated (p PHB-accumulating Bacillus sp. JL47 in shrimp was due to its capacity to stimulate the innate immune related genes of the shrimp, specifically the proPO and TGase genes. The application of probiotic Bacillus species, capable of accumulating a significant amount of PHB, is suggested as potential immunostimulatory strategy for aquaculture. Copyright © 2017 Elsevier Ltd. All rights reserved.

  18. The effect of gamma irradiation and frozen storage time on the quality of tiger shrimps (Penaeus Monodon)

    International Nuclear Information System (INIS)

    Mohamad Khan Ayob; Azizah Ali; Jalil Kadir

    1986-01-01

    The effect of gamma irradiation (0, 1, 2, 4 and kGy) and frozen storage (-10 0 C) of 6 months on the microbiological, chemical and organoleptic qualities of whole (WSI) and peeled (PS2) tiger shrimps (Peneaus monodon) were studied. The qualities of the products were compared with blanched shrimps (PSI) ordinarily prepared at a factory. The irradiation dose up to 6 kGy reduced the total bacterial count (TBC) by at least 3 log cycles with D value = 2.13 kGy. The survival of pathogenic microbes was effectively retarded. Although blanching eliminated the pathogenic microbes, the TBC of PSI before storage is still in a range of 10 5 /g. There was an increase in TBC of 1-2 log cycles during storage in all samples. Peeling effectively reduced the TBC of the shrimps. Gamma irradiation and shorter period of storage time did not influence the pH and protein contents of the products. Indole production was slowed down by irradiation at 1-4 kGy, which is comparable to blanching treatment. Organoleptic qualities (colour, ordour and texture) of the products were not effected by irradiation and storage time except for samples irradiated at 6 kGy in which the pink colour faded. There was no significant difference in the organoleptic qualities of irradiated (2 kGy) samples as compared to blanched products. (author)

  19. Dinamika Tata Kelola Kebijakan Industri Penyiaran dan Telekomunikasi Indonesia Menuju Konvergensi: Sebuah Studi Paradigma Interpretatif

    Directory of Open Access Journals (Sweden)

    Vience Mutiara Rumata

    2017-03-01

    Konvergensi antara penyiaran dan telekomunikasi di Indonesia tidak terhindarkan seiring dengan perkembangan teknologi informasi dan komunikasi (TIK serta ketatnya persaingan dalam industri keduanya. Kedua industri telah mengintegrasi TIK sebagai nilai tambah dari layanan ataupun produk yang ditawarkannya. Meski demikian, tata kelola kedua industri ini masih terpisah yakni melalui UU Penyiaran tahun 2002 dan UU Telekomunikasi tahun 1999. Di saat RUU konvergensi telematika belum menemukan titik terang, regulator berupaya merevisi kedua UU tersebut. Studi ini merupakan penelitian deskriptif kualitatif dengan kerangka paradigma interpretatif dengan berlandaskan teori neo-institusionalisme, yakni historical institutionalism. Studi ini bertujuan untuk memahami relasi kekuasaan serta pandangan para pemangku kebijakan dalam mengatur industri penyiaran dan telekomunikasi di era konvergensi. Teknik pengumpulan data dilakukan dengan cara wawancara mendalam kepada narasumber yang dipilih secara purposif yakni Kemkominfo, KPI, BRTI, dan DPR, selama Januari-Februari 2015. Setiap institusi memiliki path dependence yang berbeda antar satu dengan yang lain, sehingga membentuk pola distribusi kekuasan berbeda dalam prosedur pembuatan kebijakan. Proposal revisi UU baik dari Kemkominfo dan KPI tidak mengatur hal-hal yang berkaitan dengan konvergensi. Akan tetapi, perebutan otoritas antar kedua institusi tersebut justru menjadi fokus dalam proposal tersebut. Kemkominfo, sebagai inisiator UU Telekomunikasi, berencana untuk mengubah revisi UU tersebut menjadi UU konvergensi. Fokus utama revisi tersebut adalah upaya untuk memfasilitasi interkoneksi dan internetworking dalam infrastruktur digital sebagai fondasi ekosistem konvergensi.     Abstract The convergence between broadcasting and telecommunication in Indonesia is envitable as the growing information and communication technology and the growing competition in both industries. These industries have integrated ICT as part of their

  20. The efficacy of Poly-β-Hydroxy Butyrate (PHB)/biosurfactant derived from Staphylococcus hominis against White Spot Syndrome Virus (WSSV) in Penaeus monodon.

    Science.gov (United States)

    Monica, M; Priyanka, T; Akshaya, Murugesan; Rajeswari, V; Sivakumar, Lingappa; Somasundaram, S T; Shenbhagarathai, R

    2017-12-01

    White Spot Syndrome Virus (WSSV) is one of the most important causative agents of Penaeid shrimps diseases that incur heavy losses to the shrimp aquaculture. It has severe impact on the sustainability and the production of Penaeus monodon. Hence, the present study focussed on the investigation of Poly-β-hydroxybutyrate/biosurfactant as immunostimulants against WSSV infected shrimps. Infection of WSSV was periodically checked in all the experimental shrimps using PCR diagnostic kit. After ensuring all shrimps were free of viral infection, experiments were carried out to analyze the nonspecific immune responses (prophenol oxidase, nitro blue tetrazolium reduction assay and total haemocyte count) both in control and experimental group. Further, gills and muscles of Penaeus monodon were subjected to proteome analysis after treated it with PHB/biosurfactant independently in the concentration of 2% and 5% each. Increase in the level of haemocytes was observed in both PHB (26 ± 2 × 10⁴ cells)/biosurfactant (28 ± 2 × 10 4  cells) treated shrimps, when compared with control (17 ± 2 × 10⁴ cells). proPhenolOxidase (proPO) activity was also enhanced in treated groups compared to WSSV infected shrimps. Less production of superoxide anion was observed in control and treated groups. Differences in the protein expression was analyzed in muscle tissue of control, WSSV infected and PHB/biosurfactant treated shrimps. Our finding suggested that partial substitution of feed with 2% PHB and biosurfactant showed increased rate on the survival of WSSV infected P. monodon which might be due to either the over expression/down regulation of proteins that play a vital role in enhancing the immune system/the progression of the disease respectively. Copyright © 2017 Elsevier Ltd. All rights reserved.

  1. MANAJEMEN PERPUSTAKAAN SEKOLAH BERBASIS SISTEM INFORMASI GUNA MENINGKATKAN KEMANDIRIAN DAN OPTIMALISASI TATA KELOLA PERPUSTAKAAN SEKOLAH DASAR SE-KECAMATAN JATINANGOR

    Directory of Open Access Journals (Sweden)

    Tine Silvana

    2014-11-01

    ABSTRAK Kegiatan Pengabdian Masyarakat ini dilaksanakan di Kecamatan Jatinangor dengan mengambil tempat pelaksanaan di Aula Kantor UPTD TK, SD dan PNF Kecamatan Jatinangor dan di Ruang Pertemuan GERKOPIN Kecamatan Jatinangor. Sebagai Masyarakat Mitra dalam PKM ini yakni Unit Pengelola Teknis Dinas TK, SD dan PNF Kecamatan Jatinangor. Pencapaian Sasaran PKM ini adalah para Pengelola Perpustakaan Sekolah Dasar Se-Kecamatan Jatinangor. Kegiatan PKM yang telah dilaksanakan yakni dimulai dari kegiatan FGD untuk mengetahui kebutuhan pencapaian sasaran, kemudian Seminar dan Workshop yang diikuti oleh para Kepala Sekolah Dasar serta para Pengelola Perpustakaan dari 29 SD Negeri maupun Swasta. Berikutnya kegiatan Monitoring dan Evaluasi Akhir. Tujuan dari Program PKM ini adalah agar pencapaian sasaran dapat memiliki pengetahuan dan keterampilan teknis dalam mengelola dan mengolah koleksi bahan pustaka maupun layanan perpustakaan sekolah dengan menggunakan sistem informasi perpustakaan yang lebih mudah digunakan. Dalam kegiatan FGD diketahui bahwa hampir semua pengelola perpustakaan sekolah belum dapat menangani pengolahan koleksi secara sistematis. Meskipun sebagian diantara mereka sudah mengetahui adanya sistem informasi yang dapat digunakan untuk mengolah dan mengelola koleksi, mereka belum mengetahui dan belum dapat memanfaatkan dan menggunakannya. Dalam sesi Seminar, para Kepala Sekolah mendapatkan pemahaman lebih lanjut tentang pentingnya mengelola perpustakaan sekolah secara lebih profesional. Terlebih lagi bahwa mereka saat ini mulai dituntut untuk selalu siap menyediakan data dan informasi tentang tata kelola perpustakaan baik untuk pelaporan dan evaluasi maupun untuk kepentingan akreditasi sekolah. Dalam sesi Workshop, para peserta yang umumnya para pelaksana pengelola perpustakaan mendapatkan pengetahuan dan keterampilan teknis tentang tata cara pengolahan koleksi yang terdiri dari inventarisasi, klasifikasi, katalogisasi, pelayanan serta administrasi perpustakaan

  2. PENGARUH DISIPLIN KERJA, MOTIVASI KERJA, DAN TATA RUANG KANTOR TERHADAP KINERJA PEGAWAI KELURAHAN KECAMATAN PURBALINGGA KABUPATEN PURBALINGGA

    Directory of Open Access Journals (Sweden)

    Masasti Suci

    2015-11-01

    Full Text Available Kinerja adalah suatu hasil kerja baik secara kualitas maupun kuantitas yang dicapai oleh seseorang untuk mencapai tujuan tertentu. Kinerja yang baik tentunya dipengaruhi oleh beberapa faktor, seperti disiplin kerja, motivasi kerja, dan tata ruang kantor yang ada di kantor Kelurahan Kecamatan Purbalingga Kabupaten Purbalingga. Permasalahan dalam penelitian ini yaitu kedisiplinan pegawai dalam jam kerja yang kurang baik, rendahnya semangat pegawai untuk memberikan pelayanan kepada masyarakat dengan maksimal, masih terdapat ketidakteraturan dalam hal penataan ruang kantor dan lingkungan fisik. Metode pengumpulan data yang digunakan adalah metode angket dan dokumentasi. Uji keberartian persamaan regresi dilihat dari uji Fhitung = 49,993 dengan probabilitas sebesar 0,000 < 0,05 sehingga diperoleh hasil analisis regresi linear berganda dengan persamaan Y = 0,281 + 0,357X1 + 0,252X2 + 0,222X3. Besarnya pengaruh secara simultan antara disiplin kerja, motivasi kerja, dan tata ruang kantor terhadap kinerja pegawai yaitu 59,2%. Variabel yang memberikan pengaruh paling besar terhadap kinerja pegawai secara parsial adalah disiplin kerja yaitu sebesar 21,34%, sedangkan untuk motivasi kerja, dan tata ruang kantor berpengaruh lebih kecil yaitu masing-masing sebesar 19,53%, dan 9,18%. A performance is the work result either qualitatively or quantitatively achieved by a person to reach the certain goal. The good performance is influenced by some factors such as work discipline, work motivation, and office room arrangement in the village office. The problems of this reserach were the low disipline of civil servants’ in the work time, the low motivation of the civil servants to give the service for the guests, and the bad ordered office room arrangement and the physical environment. The data were colleted by questionnaire and documentation. The significance test of regression was found out from F test = 49.993 with probability was 0.000 < 0.05, therefore the

  3. Effect of SKT-b Vibrio probiotic bacteria addition at different developmental stages on tiger shrimp Penaeus monodon larvae survival rate

    Directory of Open Access Journals (Sweden)

    . Widanarni

    2009-07-01

    Full Text Available This experiment was conducted to study the effect of probiotic bacteria SKT-b Vibrio addition at different developmental stages on survival rates of tiger shrimp Penaeus monodon larva.  Main activity of this experiment consisted of tiger shrimp rearing started from nauplius stage until Pl10 and addition of 106 CFU/ml SKT-b Vibrio probiotic bacteria at various developmental stages namely at early nauplius stage, early zoea zoea stage, early mysis stage, early postlarva (Pl stage, every developmental stage changes, everyday, and control (without probiotic bacteria addition. Results showed that survival rates of shrimp larva ranged at 24,17%-35,83% with the higest value in the treatment of probiotic bacteria addition at every developmental stage changes namely 35,83%, whereas the lowest was found at control (without probiotic bacteria addition namely 24,17%.  No significantly different was found in term of shrimp growth rate among control and treatment. Growth rate in length of tiger shrimp larva ranged 18,64%-19,09% for SKT-b Vibrio addition and 18,47% for control. Key word: Probiotic bacteria, SKT-b Vibrio, Penaeus monodon, shrimp larvae stages   ABSTRAK Penelitian ini dilakukan untuk mempelajari pengaruh pemberian bakteri probiotik Vibrio SKT-b pada stadia yang berbeda terhadap kelangsungan hidup larva udang windu Penaeus monodon. Kegiatan utama dari penelitian ini adalah pemeliharaan udang windu yang dimulai dari stadia nauplius sampai Pl10 dan diberi bakteri probiotik Vibrio SKT-b dengan dosis 106 CFU/ml pada waktu yang berbeda yaitu pada awal stadia nauplius, awal stadia zoea, awal stadia mysis, awal stadia postlarva (Pl, setiap pergantian stadia, setiap hari, dan kontrol (tanpa pemberian bakteri probiotik. Hasil penelitian yang diperoleh menunjukkan bahwa tingkat kelangsungan hidup larva udang berkisar antara 24,17%-35,83% dengan nilai tertinggi terdapat pada perlakuan pemberian bakteri probiotik pada setiap pergantian stadia yaitu

  4. Effect of Adding the White Legged Shrimp (Penaeus vannamei) on Growth and Survival of Tiger Shrimp (Penaeus monodon) in Intensive Farming

    OpenAIRE

    . Tarsim

    2007-01-01

    This experiment was carried out in the framework of a project to develop a viable shrimp polyculture technology under intensive farming. The objectives were to assess the effect of adding white legged shrimp (Penaeus vannamei) on growth and survival of tiger shrimp (Penaeus monodon) in intensive Farming. Tiger shrimp were stocked in 2800-3400 m2 earthen ponds at 40 individuals/m2 and reared for 133 days. White legged shrimp added at 70 day at 8±2 individuals/m2. Growth rate and total yield af...

  5. PEMAHAMAN, SIKAP, DAN UNJUK KERJA HIGIENE-SANITASI SISWA DALAM PENGOLAHAN DAN PENYAJIAN MAKANAN DI SMK BIDANG KEAHLIAN TATA BOGA

    Directory of Open Access Journals (Sweden)

    Luh Masdarini

    2012-08-01

    Full Text Available The gastronomy students’ hygiene-sanitation under standing, attitude and performance in SMK. This study aims at describing the gastronomy students’ hygiene-sanitation understanding, attitude and performance. This research is a des­crip­tive quantitative one with research subjects are the gastronomy students of SMKs in Bali with the total number of 198 students. The instruments used are skill test, questionnaire and observation sheet. Then, the data are analyzed descriptively by using percentage formula. The data analysis shows that: (1 the gastronomy students’ understanding over the hygiene-sanitation 82,89% is very good, (2 the gas­tronomy stu­dents’ attitude over the hygiene-sanitation 84,36% is very good, (3 the gastronomy students’ performance over the hygiene-sanitation 90,20% is very good. Pemahaman, Sikap, dan Unjuk Kerja Higiene-sanitasi Siswa dalam Peng­olahan dan Penyajian Makanan di SMK Bidang Keahlian Tata Boga. Tujuan penelitian ini untuk mendeskripsikan pemahaman, sikap, dan unjuk kerja higiene-sani­tasi siswa dalam pengolahan dan penyajian makanan. Penelitian ini menggunakan rancangan penelitian deskriptif kuantitatif dengan subjek penelitian siswa di SMK Bidang Keahlian Tata Boga di Bali yang berjumlah 198 orang. Instrumen penelitian yang digunakan adalah tes pengetahuan, angket, dan lembar observasi. Selanjutnya data dianalisis dengan teknik deskriptif dengan menggunakan formula persentase. Hasil penelitian ini menunjukkan bahwa: (1 pemahaman higiene-sanitasi siswa dalam pengolahan dan penyajian makanan 82,89% adalah sangat baik, (2 Sikap higiene-sanitasi siswa dalam pengolahan dan penyajian makanan 84,36% adalah sangat baik, (3 Unjuk kerja higiene-sanitasi siswa dalam pengolahan dan penyajian makanan 90,20% adalah sangat baik.

  6. White spot syndrome virus isolates of tiger shrimp Penaeus monodon (Fabricious) in India are similar to exotic isolates as revealed by polymerase chain reaction and electron microscopy.

    Science.gov (United States)

    Mishra, S S; Shekhar, M S

    2005-07-01

    Microbiological analysis of samples collected from cases of white spot disease outbreaks in cultured shrimp in different farms located in three regions along East Coast of India viz. Chidambram (Tamil Nadu), Nellore (Andhra Pradesh) and Balasore (Orissa), revealed presence of Vibrio alginolyticus, Vibrio parahaemolyticus, and Aeromonas spp. but experimental infection trials in Penaeus monodon with these isolates did not induce any acute mortality or formation of white spots on carapace. Infection trials using filtered tissue extracts by oral and injection method induced mortality in healthy P. monodon with all samples and 100% mortality was noted by the end of 7 day post-inoculation. Histopathological analysis demonstrated degenerated cells characterized by hypertrophied nuclei in gills, hepatopancreas and lymphoid organ with presence of intranuclear basophilic or eosino-basophilic bodies in tubular cells and intercellular spaces. Analysis of samples using 3 different primer sets as used by other for detection of white spot syndrome virus (WSSV) generated 643, 1447 and 520bp amplified DNA products in all samples except in one instance. Variable size virions with mean size in the range of 110 x 320 +/- 20 nm were observed under electron microscope. It could be concluded that the viral isolates in India involved with white spot syndrome in cultured shrimp are similar to RV-PJ and SEMBV in Japan, WSBV in Taiwan and WSSV in Malaysia, Indonesia, Thailand, China and Japan.

  7. Novel alternative to antibiotics in shrimp hatchery: effects of the essential oil of Cinnamosma fragrans on survival and bacterial concentration of Penaeus monodon larvae.

    Science.gov (United States)

    Randrianarivelo, R; Danthu, P; Benoit, C; Ruez, P; Raherimandimby, M; Sarter, S

    2010-08-01

    The activity of two essential oils (EOs) of Cinnamosma fragrans, an endemic plant to Madagascar (B8: linalool-type and B143: 1,8-cineole-type), against bacterial isolates from a shrimp hatchery of Penaeus monodon and their effects on the survival and bacterial concentration of larvae were determined. Minimum inhibitory concentrations were determined using a broth dilution technique. The bacterial concentrations of both larvae and water tank were assessed on Marine agar and Thiosulfate Citrate Bile Sucrose agar. The assays took place in OSO Farming's shrimp hatchery in Madagascar. EOs were directly added to the water tank. Regarding the survival, the assays in larval culture (four replicates each of B8, B143, E and control) showed that B8 oil had a similar effect (P > 0.05) as the antibiotic (Erythromycin) and was more active than B143 (P larvae for all assays. Both C. fragrans essential oils, as antibiotic, exhibited significantly higher survival rates and lower bacterial concentrations of the larvae than the control (oil and antibiotic free). The potential of C. fragrans essential oil to control the bacterial load in in vivo conditions, thereby enhancing survival rate of P. monodon larvae, makes it a relevant option for developing a novel alternative to antibiotics in shrimp hatchery culture.

  8. Bioaccumulation and public health implications of trace metals in edible tissues of the crustaceans Scylla serrata and Penaeus monodon from the Tanzanian coast.

    Science.gov (United States)

    Rumisha, Cyrus; Leermakers, Martine; Mdegela, Robinson H; Kochzius, Marc; Elskens, Marc

    2017-09-30

    The coastal population in East Africa is growing rapidly but sewage treatment and recycling facilities in major cities and towns are poorly developed. Since estuarine mangroves are the main hotspots for pollutants, there is a potential for contaminants to accumulate in edible fauna and threaten public health. This study analysed trace metals in muscle tissues of the giant mud crabs (Scylla serrata) and the giant tiger prawns (Penaeus monodon) from the Tanzanian coast, in order to determine the extent of bioaccumulation and public health risks. A total of 180 samples of muscle tissues of S. serrata and 80 of P. monodon were collected from nine sites along the coast. Both species showed high levels of trace metals in the wet season and significant bioaccumulation of As, Cu and Zn. Due to their burrowing and feeding habits, mud crabs were more contaminated compared to tiger prawns sampled from the same sites. Apart from that, the measured levels of Cd, Cr and Pb did not exceed maximum limits for human consumption. Based on the current trend of fish consumption in Tanzania (7.7 kg/person/year), the measured elements (As, Cd, Co, Cu, Mn, Pb and Zn) are not likely to present health risks to shellfish consumers. Nevertheless, potential risks of As and Cu cannot be ruled out if the average per capita consumption is exceeded. This calls for strengthened waste management systems and pollution control measures.

  9. PmVRP15, a Novel Viral Responsive Protein from the Black Tiger Shrimp, Penaeus monodon, Promoted White Spot Syndrome Virus Replication

    Science.gov (United States)

    Vatanavicharn, Tipachai; Prapavorarat, Adisak; Jaree, Phattarunda; Somboonwiwat, Kunlaya; Tassanakajon, Anchalee

    2014-01-01

    Suppression subtractive hybridization of Penaeus monodon hemocytes challenged with white spot syndrome virus (WSSV) has identified the viral responsive gene, PmVRP15, as the highest up-regulated gene ever reported in shrimps. Expression analysis by quantitative real time RT-PCR revealed 9410–fold up-regulated level at 48 h post WSSV injection. Tissue distribution analysis showed that PmVRP15 transcript was mainly expressed in the hemocytes of shrimp. The full-length cDNA of PmVRP15 transcript was obtained and showed no significant similarity to any known gene in the GenBank database. The predicted open reading frame of PmVRP15 encodes for a deduced 137 amino acid protein containing a putative transmembrane helix. Immunofluorescent localization of the PmVRP15 protein revealed it accumulated around the nuclear membrane in all three types of shrimp hemocytes and that the protein was highly up-regulated in WSSV-infected shrimps. Double-stranded RNA interference-mediated gene silencing of PmVRP15 in P. monodon significantly decreased WSSV propagation compared to the control shrimps (injected with GFP dsRNA). The significant decrease in cumulative mortality rate of WSSV-infected shrimp following PmVRP15 knockdown was observed. These results suggest that PmVRP15 is likely to be a nuclear membrane protein and that it acts as a part of WSSV propagation pathway. PMID:24637711

  10. Revealing localization and regulation of GTPase PmRab7 in lymphoid cells of Penaeus monodon after WSSV infection

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    Amrendra Kumar

    2016-10-01

    Full Text Available Objective: To identify white spot syndrome virus (WSSV entry into the host-cells of the cultured shrimp Penaeus monodon, we have attempted to localize PmRab7 (Ras-related in brain which is playing a vital role in the WSSV internalization. Methods: In this study, we have cloned PmRab7 and expressed in Escherichia coli, further purified rPmRab7 was used for antibody production, isolation of lysosomal sub-cellular fractions and western blot against lysosomal protein. Moreover, high fold-change in PmRab7 regulation with increasing copy number of WSSV has been studied by using real-time PCR. Results: 651 bp amplicon size gene was successfully amplified, ligated amplicon with pTZ T-tail vector confirmed by colony PCR and retriction enzyme digestion on agarose gel. Subcloned (pRSET-B 651 bp gene transformed successfully in Rosetta and after 6 h of induction expressed rPmRab7 was on SDS page, furthermore soluble fraction of rPmRab7 (26 kDa was purified by ni-NTA column. AntiPmRab7 antibody was received by Merk Pvt. Ltd., and western blot analysis revealed that PmRab7 is present in the lysosomal sub-cellular fraction. Copy number of WSSV was increased 5 fold in 24 h and 20 fold in 72 h of infection and subsequently transcrtipt of PmRab7 was Ct = 1.0 to Ct = 8.5. Conclusions: Presence of PmRab7 on lysosome clearly indicating PmRab7 participating in lysosomal maturation, other hand WSSV may follow the same route of entry. WSSV internalization has directly linked with regulation of PmRab7.

  11. Attempts on producing lymphoid cell line from Penaeus monodon by induction with SV40-T and 12S EIA oncogenes.

    Science.gov (United States)

    Puthumana, Jayesh; Prabhakaran, Priyaja; Philip, Rosamma; Singh, I S Bright

    2015-12-01

    In an attempt of in vitro transformation, transfection mediated expression of Simian virus-40 (T) antigen (SV40-T) and transduction mediated expression of Adenovirus type 12 early region 1A (12S E1A) oncogene were performed in Penaeus monodon lymphoid cells. pSV3-neo vector encoding SV40-T oncogene and a recombinant baculovirus BacP2-12S E1A-GFP encoding 12S E1A oncogene under the control of hybrid promoters were used. Electroporation and lipofection mediated transformation of SV40-T in lymphoid cells confirmed the transgene expression by phenotypic variation and the expression of GFP in co-transfection experiment. The cells transfected by lipofection (≥ 5%) survived for 14 days with lower toxicity (30%), whilst on electroporation, most of the cells succumbed to death (60%) and survived cells lived up to 7 days. Transduction efficiency in primary lymphoid cells was more than 80% within 14 days of post-transduction, however, an incubation period of 7 days post-transduction was observed without detectable expression of 12S E1A. High level of oncogenic 12S E1A expression were observed after 14 day post-transduction and the proliferating cells survived for more than 90 days with GFP expression, however, without in vitro transformation and immortalization. The study put forth the requirement of transduction mediated 'specific' oncogene expression along with telomerase activation and epigenetic induction for the immortalization and establishment of shrimp cell line. Copyright © 2015. Published by Elsevier Ltd.

  12. Gene expression profiling in gill tissues of White spot syndrome virus infected black tiger shrimp Penaeus monodon by DNA microarray.

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    Shekhar, M S; Gomathi, A; Gopikrishna, G; Ponniah, A G

    2015-06-01

    White spot syndrome virus (WSSV) continues to be the most devastating viral pathogen infecting penaeid shrimp the world over. The genome of WSSV has been deciphered and characterized from three geographical isolates and significant progress has been made in developing various molecular diagnostic methods to detect the virus. However, the information on host immune gene response to WSSV pathogenesis is limited. Microarray analysis was carried out as an approach to analyse the gene expression in black tiger shrimp Penaeus monodon in response to WSSV infection. Gill tissues collected from the WSSV infected shrimp at 6, 24, 48 h and moribund stage were analysed for differential gene expression. Shrimp cDNAs of 40,059 unique sequences were considered for designing the microarray chip. The Cy3-labeled cRNA derived from healthy and WSSV-infected shrimp was subjected to hybridization with all the DNA spots in the microarray which revealed 8,633 and 11,147 as up- and down-regulated genes respectively at different time intervals post infection. The altered expression of these numerous genes represented diverse functions such as immune response, osmoregulation, apoptosis, nucleic acid binding, energy and metabolism, signal transduction, stress response and molting. The changes in gene expression profiles observed by microarray analysis provides molecular insights and framework of genes which are up- and down-regulated at different time intervals during WSSV infection in shrimp. The microarray data was validated by Real Time analysis of four differentially expressed genes involved in apoptosis (translationally controlled tumor protein, inhibitor of apoptosis protein, ubiquitin conjugated enzyme E2 and caspase) for gene expression levels. The role of apoptosis related genes in WSSV infected shrimp is discussed herein.

  13. OPTIMISATION OF SUPERCRITICAL FLUID EXTRACTION OF ASTAXANTHIN FROM PENAEUS MONODON WASTE USING ETHANOL-MODIFIED CARBON DIOXIDE

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    SHAZANA A. RADZALI

    2016-05-01

    Full Text Available Some studies demonstrated that astaxanthin surpasses the antioxidant benefits of beta-carotene, zeaxanthin, canthaxanthin, vitamin C, and vitamin E. Penaeus monodon (Tiger shrimp is one of the most valuable traded crustacean products in which astaxanthin can be found in its by-products. The extraction of thermolabile compound like carotenoids at lower temperatures through supercritical carbon dioxide (SC-CO2 can reduce the potential isomerization and degradation of the extraction product. In this study, astaxanthin had been extracted using SC-CO2 with 15% (v/v ethanol as an entrainer and the recovered astaxanthin was analyzed using High performance liquid chromatography (HPLC. A central composite design (CCD was employed to study the effect of three SC-CO2 parameters namely temperature (X1 from 40 to 80°C, pressure (X2 from 150 to 250 bar and extraction flow rate (X3 from 1 to 3 ml/min on the astaxanthin complex yield, (Y1 and free astaxanthin content, (Y2. The nonlinear regression equations were significantly (p0.9261, which had no indication of lack of fit. The results indicated that a combined set of values of temperature (56.88°C, pressure (215.68 bar and extraction flow rate (1.89 ml/min was predicted to provide the optimum region in terms of astaxanthin complex yield, (58.50 ± 2.62 µg/g and free astaxanthin content (12.20 ± 4.16 µg/g studied.

  14. PENGARUH KEPEMIMPINAN SENIOR, TATA KELOLA DAN TANGGUNG JAWAB SOSIAL TERHADAP KINERJA KEPALA RUANG RAWAT INAP RUMAH SAKIT KARYA BHAKTI KOTA BOGOR TAHUN 2008

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    Adila Kasni Astiena

    2010-09-01

    Full Text Available AbstrakPenelitian ini bertujuan untuk mengetahui pengaruh Kepemimpinan Senior, Tata Kelola dan Tanggung Jawab Sosial Terhadap Kinerja Kepala Ruang Rawat Inap Rumah Sakit Karya Bhakti (RSKB Bogor Tahun 2008. Kerangka teori dari penelitian ini diambil dari Malcolm Baldrige Criteria for Performance Excellence (MBCfPE bagi institusi kesehatan dalam Hertz (2008. Kriteria MBCfPE yang diambil adalah kepemimpinan (leadership yang dijabarkan menjadi variabel Kepemimpinan Senior, Tata Kelola Dan Tanggung Jawab Sosial. Penelitian ini merupakan penelitian survei dengan pendekatan kuantitatif. Data yang dikumpulkan adalah data primer dengan menggunakan kuesioner dan diolah dengan menggunakan metode analisis jalur (Path Analysis. Responden penelitian ini adalah semua perawat ruang rawat inap Dahlia Anyelir RSKB tahun 2008. Hasil penelitian ditemukan bahwa Kepemimpinan Senior, Tata Kelola dan Tanggung Jawab Sosial mempengaruhi Kinerja Kepala Ruang sebesar 57.59% sedangkan sisanya 42.41% dipengaruhi oleh variabel yang tidak diteliti. Variabel yang paling besar mempengaruhi kinerja kepala ruang adalah kepemimpinan senior (30.44% disusul oleh variabel tata kelola (22.96% dan Tanggung Jawab Sosial (4.18%. Tanggung Jawab Sosial mempunyai koefisen jalur yang tidak bermakna dan sangat kecil, namun tetap dipertahankan dalam model akhir karena secara substantif, penting dalam menentukan kinerja kepala ruang. Berdasarkan penelitian ini disarankan untuk lebih memperhatikan dan meningkatkan kepemimpinan senior, tata kelola dan tanggung jawab sosial guna meningkatkan kinerja kepala ruang dengan cara (1 melakukan pembinaan dalam hal kepemimpinan mencakup kemampuan (ability, keterampilan (skill dan perilaku (behaviour. (2 Menciptakan kebijakan guna terciptanya kondisi peningkatan kemampuan kepemimpinan senior, tata kelola dan tanggung jawab sosial, termasuk memberikan kesempatan untuk menambah pengetahuan (3 Dalam pemilihan kepala ruang disarankan untuk memperhatikan

  15. Interaction between TATA-Binding Protein (TBP and Multiprotein Bridging Factor-1 (MBF1 from the Filamentous Insect Pathogenic Fungus Beauveria bassiana.

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    Chi Song

    Full Text Available TATA-binding protein (TBP is a ubiquitous component of eukaryotic transcription factors that acts to nucleate assembly and position pre-initiation complexes. Multiprotein bridging factor 1 (MBF1 is thought to interconnect TBP with gene specific transcriptional activators, modulating transcriptional networks in response to specific signal and developmental programs. The insect pathogen, Beauveria bassiana, is a cosmopolitan fungus found in most ecosystems where it acts as an important regulator of insect populations and can form intimate associations with certain plants. In order to gain a better understanding of the function of MBF1 in filamentous fungi, its interaction with TBP was demonstrated. The MBF1 and TBP homologs in B. bassiana were cloned and purified from a heterologous E. coli expression system. Whereas purified BbTBP was shown to be able to bind oligonucleotide sequences containing the TATA-motif (Kd ≈ 1.3 nM including sequences derived from the promoters of the B. bassiana chitinase and protease genes. In contrast, BbMBF1 was unable to bind to these same target sequences. However, the formation of a ternary complex between BbMBF1, BbTBP, and a TATA-containing target DNA sequence was seen in agarose gel electrophoretic mobility shift assays (EMSA. These data indicate that BbMBF1 forms direct interactions with BbTBP, and that the complex is capable of binding to DNA sequences containing TATA-motifs, confirming that BbTBP can link BbMBF1 to target sequences as part of the RNA transcriptional machinery in fungi.

  16. The cultivation of medicinal, aromatic and spice plants in South Marocco. Relation to a trip to the regions between Agadir - Marrakech - Ouarzazate and Tata

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    Ait Chitt, M.

    1985-01-01

    Full Text Available Studies about aromatic, medicinal and seasoning plants are integrated into activities of Department of "Vegetables Cultures" of the Section of Agadir (Agronomical and Veterinary Institute Hassan II. An explanatory mission has been carried out in the provinces of Agadir and Marrakech in semi-barren areas and of Ouarzazate and Tata in Saharan regions. The mean cultivated species are listed. A technical and economical approach is considered.

  17. Development of SYBR Green and TaqMan quantitative real-time PCR assays for hepatopancreatic parvovirus (HPV) infecting Penaeus monodon in India.

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    Yadav, Reena; Paria, Anutosh; Mankame, Smruti; Makesh, M; Chaudhari, Aparna; Rajendran, K V

    2015-12-01

    Hepatopancreatic parvovirus (HPV) infects Penaeus monodon and causes mortality in the larval stages. Further, it has been implicated in the growth retardation in cultured P. monodon. Though different geographical isolates of HPV show large sequence variations, a sensitive PCR assay specific to Indian isolate has not yet been reported. Here, we developed a sensitive SYBR Green-based and TaqMan real-time PCR for the detection and quantification of the virus. A 441-bp PCR amplicon was cloned in pTZ57 R/T vector and the plasmid copy number was estimated. A 10-fold serial dilution of the plasmid DNA from 1 × 10(9) copies to 1 copy was prepared and used as the standard. The primers were tested initially using the standard on a conventional PCR format to determine the linearity of detection. The standards were further tested on real-time PCR format using SYBR Green and TaqMan chemistry and standard curves were generated based on the Ct values from three well replicates for each dilution. The assays were found to be sensitive, specific and reproducible with a wide dynamic range (1 × 10(9) to 10 copies) with coefficient of regression (R(2)) > 0.99, calculated average slope -3.196 for SYBR Green assay whereas, for TaqMan assay it was >0.99 and -3.367, respectively. The intra- and inter-assay variance of the Ct values ranged from 0.26% to 0.94% and 0.12% to 0.81%, respectively, for SYBR Green assay, and the inter-assay variance of the Ct values for TaqMan assay ranged from 0.07% to 1.93%. The specificity of the assays was proved by testing other DNA viruses of shrimp such as WSSV, IHHNV and MBV. Standardized assays were further tested to detect and quantify HPV in the post-larvae of P. monodon. The result was further compared with conventional PCR to test the reproducibility of the test. The assay was also used to screen Litopeneaus vannamei, Macrobrachium rosenbergii and Scylla serrata for HPV. Copyright © 2015 Elsevier Ltd. All rights reserved.

  18. Democracy leadership (study approach of bureaucrats leadership at sub bagian tata usaha badan perencanaan pembangunan daerah, East Java province)

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    Lestari, Y.; Rosdiana, W.; Noviyanti

    2018-01-01

    The main key to organizational success depends on the success of a leadership. Each organization’s progress will require the ability of a leader to transformed the organization. The emergence of the democratic-leadership is one of the most humane style of leadership. Democratic leadership positioned people as the most important factor in the leadership exercised by the orientation and emphasis on relationships with members of the organization. This study raised that the democratic-leadership in government agencies to study the leadership approach of bureaucrats at Sub Bagian Tata Usaha Badan Perencanaan Pembangunan Daerah East Java Province. The data collection techniques used descriptive research with qualitative approach, then the techniques were interviews, observation and documentation. While, the research data analysis used interactive analysis model approach of Miles and Huberman, which includes: (1) data reduction; (2) the presentation of the data; and (3) conclusion. Based on Nawawi theory’s [1], this study showed that (1) Leaders are very obedient to the rules/procedures work, (2) Leaders look more autocratic, (3) leaders make familiarity with subordinates, (4) leaders develop kinship situation and teamwork, (5) the leaders seem monotonous work and do not like a modification, (6) Leaders seem slow in decision making, and (7) leaders are accustomed to low-risk jobs with less survival trends.

  19. Mediator, TATA-binding Protein, and RNA Polymerase II Contribute to Low Histone Occupancy at Active Gene Promoters in Yeast*

    Science.gov (United States)

    Ansari, Suraiya A.; Paul, Emily; Sommer, Sebastian; Lieleg, Corinna; He, Qiye; Daly, Alexandre Z.; Rode, Kara A.; Barber, Wesley T.; Ellis, Laura C.; LaPorta, Erika; Orzechowski, Amanda M.; Taylor, Emily; Reeb, Tanner; Wong, Jason; Korber, Philipp; Morse, Randall H.

    2014-01-01

    Transcription by RNA polymerase II (Pol II) in eukaryotes requires the Mediator complex, and often involves chromatin remodeling and histone eviction at active promoters. Here we address the role of Mediator in recruitment of the Swi/Snf chromatin remodeling complex and its role, along with components of the preinitiation complex (PIC), in histone eviction at inducible and constitutively active promoters in the budding yeast Saccharomyces cerevisiae. We show that recruitment of the Swi/Snf chromatin remodeling complex to the induced CHA1 promoter, as well as its association with several constitutively active promoters, depends on the Mediator complex but is independent of Mediator at the induced MET2 and MET6 genes. Although transcriptional activation and histone eviction at CHA1 depends on Swi/Snf, Swi/Snf recruitment is not sufficient for histone eviction at the induced CHA1 promoter. Loss of Swi/Snf activity does not affect histone occupancy of several constitutively active promoters; in contrast, higher histone occupancy is seen at these promoters in Mediator and PIC component mutants. We propose that an initial activator-dependent, nucleosome remodeling step allows PIC components to outcompete histones for occupancy of promoter sequences. We also observe reduced promoter association of Mediator and TATA-binding protein in a Pol II (rpb1-1) mutant, indicating mutually cooperative binding of these components of the transcription machinery and indicating that it is the PIC as a whole whose binding results in stable histone eviction. PMID:24727477

  20. The effects of temperature and oxygen availability on intracapsular development of Acanthina monodon (Gastropoda: Muricidae El efecto de la temperatura y la disponibilidad de oxígeno sobre el desarrollo intracapsular de Acanthina monodon (Gastropoda: Muricidae

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    MIRIAM FERNÁNDEZ

    2006-06-01

    Full Text Available Freshwater and marine organisms show similar models of parental care and are faced with similar constraints to brood, which suggest that comparable environmental limits drive the evolution of parental care in aquatic systems. In fact, the low diffusion coefficient and solubility of oxygen in aquatic environments affect oxygen acquisition and therefore the capacity to aggregate embryos. The effect of other critical environmental variables, such as temperature, is less clear. We assessed the effects of temperature and oxygen availability on (1 the number of developed and undeveloped encapsulated embryos, (2 the proportion of embryos reaching advanced stages during intracapsular development (counting not only developed and undeveloped embryos but also abnormal embryos, (3 asynchrony in development (estimated only in capsules in which development occurred, and (4 final embryo size, as the first step toward identifying the main factors constraining parental care in the ocean. We used the gastropod Acanthina monodon as a model because it has an extended latitudinal range of distribution and exhibits feeding larvae during intracapsular development. The latter factor is relevant because previous studies have suggested that sibling cannibalism could be triggered by intracapsular competition for oxygen. Freshly laid egg capsules were collected and incubated until embryos hatched under different experimental temperatures (7, 11, 15 and 19 °C and oxygen conditions (hypoxia: 50-60 % air saturation; normoxia; and hyperoxia: 150-160 %. More embryos remained in early stages at the end of the experimental period under hypoxia and at the highest experimental temperature. The mean number of developed embryos was significantly lower under hypoxia conditions than under normoxia and hyperoxia, but was not influenced by temperature. However, temperature negatively affected embryo size of developed embryos and the level of asynchrony (number of different developmental

  1. The novel white spot syndrome virus-induced gene, PmERP15, encodes an ER stress-responsive protein in black tiger shrimp, Penaeus monodon.

    Science.gov (United States)

    Leu, Jiann-Horng; Liu, Kuan-Fu; Chen, Kuan-Yu; Chen, Shu-Hwa; Wang, Yu-Bin; Lin, Chung-Yen; Lo, Chu-Fang

    2015-04-01

    By microarray screening, we identified a white spot syndrome virus (WSSV)-strongly induced novel gene in gills of Penaeus monodon. The gene, PmERP15, encodes a putative transmembrane protein of 15 kDa, which only showed some degree of similarity (54-59%) to several unknown insect proteins, but had no hits to shrimp proteins. RT-PCR showed that PmERP15 was highly expressed in the hemocytes, heart and lymphoid organs, and that WSSV-induced strong expression of PmERP15 was evident in all tissues examined. Western blot analysis likewise showed that WSSV strongly up-regulated PmERP15 protein levels. In WSSV-infected hemocytes, immunofluorescence staining showed that PmERP15 protein was colocalized with an ER enzyme, protein disulfide isomerase, and in Sf9 insect cells, PmERP15-EGFP fusion protein colocalized with ER -Tracker™ Red dye as well. GRP78, an ER stress marker, was found to be up-regulated in WSSV-infected P. monodon, and both PmERP15 and GRP78 were up-regulated in shrimp injected with ER stress inducers tunicamycin and dithiothreitol. Silencing experiments showed that although PmERP15 dsRNA-injected shrimp succumbed to WSSV infection more rapidly, the WSSV copy number had no significant changes. These results suggest that PmERP15 is an ER stress-induced, ER resident protein, and its induction in WSSV-infected shrimp is caused by the ER stress triggered by WSSV infection. Furthermore, although PmERP15 has no role in WSSV multiplication, its presence is essential for the survival of WSSV-infected shrimp. Copyright © 2014 Elsevier Ltd. All rights reserved.

  2. GENETIC STATUS, SOURCE AND ESTABLISHMENT RISK OF THE GIANT TIGER SHRIMP (PENAEIDAE: Penaeus monodon, AN INVASIVE SPECIES IN COLOMBIAN CARIBBEAN WATERS

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    J.C. AGUIRRE-PABÓN

    2015-01-01

    El camarón tigre (Penaeus monodon es una especie del Indo-Pacífico. Su producción mundial entre 1970 y 1980 superó todas las otras especies de camarón, lo que favoreció su introducción y cultivo fuera del área de distribución natural en varios países de África, Europa, EE.UU. y América del Sur. Actualmente se encuentra en la costa del Océano Atlántico (México, Estados Unidos, Puerto Rico, Brasil, Guyana, Venezuela y Colombia. A pesar del riesgo que implica, no se han realizado estudios para evaluar su impacto como posible especie invasora y su condición genética. Este estudio evaluó el estado genético y el origen de la población de P. monodon en el norte del Caribe colombiano, analizando la región control del ADN mitocondrial (ADNmt-CR. 16 individuos fueron recolectados al azar del Golfo de Salamanca y 342 secuencias originales de muestras del Indo- Pacífico fueron obtenidas de GenBank. Se analizaron los parámetros de diversidad genética y las relaciones genéticas. Se analizaron un total de 358 secuencias y se identificaron 303 haplotipos. En la población de Colombia se identificaron tres haplotipos, mostrando una baja diversidad genética en comparación con las poblaciones del Indo-Pacífico. Estos haplotipos se encontraron cercanamente relacionados con secuencias obtenidas de muestras de Filipinas y Taiwán, principalmente. Se discute la necesidad de crear una red regional para caracterizar las poblaciones establecidas en el Gran Caribe, con el propósito de inferir los procesos de colonización y el establecimiento de medidas de manejo

  3. Facies volcánicas del depósito de avalancha de detritos del volcán Tata Sabaya, Andes Centrales Volcanic facies of the debris avalanche deposit of Tata Sabaya Volcano, Central Andes

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    Benigno Godoy

    2012-09-01

    Full Text Available Las avalanchas de detritos, asociadas a colapsos parciales de edificios volcánicos, son fenómenos comunes en la evolución de un volcán. Este tipo de flujos son por inestabilidades, que pueden deberse a factores tales como la existencia de zonas afectadas por alteración hidrotermal, cambios climáticos, terremotos, intrusión de magmas en zonas superficiales (criptodomos y/o diques y/o movimiento de fallas bajo el edificio volcánico o cercanas a él. El producto final de estos flujos -denominado depósito de avalancha de detritos (DAD- presenta morfologías típicas de cerrillos y drenajes cerrados. En los Andes Centrales se han reconocido, al menos, 14 centros volcánicos con depósitos de avalancha asociados, entre los que está el volcán Tata Sabaya (Bolivia. El colapso que ha dado origen a este depósito podría haberse generado por una combinación de actividad sísmica y magmática en el volcán. El depósito asociado al colapso parcial de este volcán se distribuye sobre la parte baja del flanco sur del volcán y sobre la parte noroccidental de la cuenca del salar de Coipasa. Cubre una superficie de más de 230 km² y tiene un volumen estimado de 6±1 km³. Sobre la base de las composiciones litológicas, se ha establecido que el depósito está constituido por 6 tipos distintos de cerrillos, los cuales son: lávicos, piroclásticos, sedimentarios mixtos, brecha piroclástica y andesítico-basálticos. Considerando el tipo predominante de cerrillos y su distribución espacial dentro del depósito, se ha definido 6 facies diferentes (Facies de Bloques Toreva, Facies de Cerrillos Volcánicos, Facies Central, Facies de Cerrillos Sedimentarios, Facies Mixta y Facies de Cerrillos de Brecha Piroclástica. Tomando en cuenta la distribución espacial de estas facies, se propone la estructura del paleovolcán previa a su colapso parcial.Debris avalanches associated with partial sector collapse of volcanic edifices are common phenomena in the

  4. TATA-binding protein-associated factor 7 regulates polyamine transport activity and polyamine analog-induced apoptosis.

    Science.gov (United States)

    Fukuchi, Junichi; Hiipakka, Richard A; Kokontis, John M; Nishimura, Kazuhiro; Igarashi, Kazuei; Liao, Shutsung

    2004-07-16

    Identification of the polyamine transporter gene will be useful for modulating polyamine accumulation in cells and should be a good target for controlling cell proliferation. Polyamine transport activity in mammalian cells is critical for accumulation of the polyamine analog methylglyoxal bis(guanylhydrazone) (MGBG) that induces apoptosis, although a gene responsible for transport activity has not been identified. Using a retroviral gene trap screen, we generated MGBG-resistant Chinese hamster ovary (CHO) cells to identify genes involved in polyamine transport activity. One gene identified by the method encodes TATA-binding protein-associated factor 7 (TAF7), which functions not only as one of the TAFs, but also a coactivator for c-Jun. TAF7-deficient cells had decreased capacity for polyamine uptake (20% of CHO cells), decreased AP-1 activation, as well as resistance to MGBG-induced apoptosis. Stable expression of TAF7 in TAF7-deficient cells restored transport activity (55% of CHO cells), AP-1 gene transactivation (100% of CHO cells), and sensitivity to MGBG-induced apoptosis. Overexpression of TAF7 in CHO cells did not increase transport activity, suggesting that TAF7 may be involved in the maintenance of basal activity. c-Jun NH2-terminal kinase inhibitors blocked MGBG-induced apoptosis without alteration of polyamine transport. Decreased TAF7 expression, by RNA interference, in androgen-independent human prostate cancer LN-CaP104-R1 cells resulted in lower polyamine transport activity (25% of control) and resistance to MGBG-induced growth arrest. Taken together, these results reveal a physiological function of TAF7 as a basal regulator for mammalian polyamine transport activity and MGBG-induced apoptosis.

  5. Mediator, TATA-binding protein, and RNA polymerase II contribute to low histone occupancy at active gene promoters in yeast.

    Science.gov (United States)

    Ansari, Suraiya A; Paul, Emily; Sommer, Sebastian; Lieleg, Corinna; He, Qiye; Daly, Alexandre Z; Rode, Kara A; Barber, Wesley T; Ellis, Laura C; LaPorta, Erika; Orzechowski, Amanda M; Taylor, Emily; Reeb, Tanner; Wong, Jason; Korber, Philipp; Morse, Randall H

    2014-05-23

    Transcription by RNA polymerase II (Pol II) in eukaryotes requires the Mediator complex, and often involves chromatin remodeling and histone eviction at active promoters. Here we address the role of Mediator in recruitment of the Swi/Snf chromatin remodeling complex and its role, along with components of the preinitiation complex (PIC), in histone eviction at inducible and constitutively active promoters in the budding yeast Saccharomyces cerevisiae. We show that recruitment of the Swi/Snf chromatin remodeling complex to the induced CHA1 promoter, as well as its association with several constitutively active promoters, depends on the Mediator complex but is independent of Mediator at the induced MET2 and MET6 genes. Although transcriptional activation and histone eviction at CHA1 depends on Swi/Snf, Swi/Snf recruitment is not sufficient for histone eviction at the induced CHA1 promoter. Loss of Swi/Snf activity does not affect histone occupancy of several constitutively active promoters; in contrast, higher histone occupancy is seen at these promoters in Mediator and PIC component mutants. We propose that an initial activator-dependent, nucleosome remodeling step allows PIC components to outcompete histones for occupancy of promoter sequences. We also observe reduced promoter association of Mediator and TATA-binding protein in a Pol II (rpb1-1) mutant, indicating mutually cooperative binding of these components of the transcription machinery and indicating that it is the PIC as a whole whose binding results in stable histone eviction. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

  6. Peluang dan Tantangan Undang-undang Desa dalam Upaya Demokratisasi Tata Kelola Sumber Daya Alam Desa: Perspektif Agraria Kritis

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    Mohamad Shohibuddin

    2016-11-01

    Full Text Available Undang-undang Nomor 6 Tahun 2014 tentang Desa (UU Desa-terlepas dari terobosan politiknya dalam menggulirkan demokratisasi relasi negara-desa-memiliki keterbatasan mendasar terkait isu sumber daya alam di desa mengingat krisis agraria dan krisis ekologi yang terjadi di pedesaan. Selain tidak banyak mengelaborasi aspek-aspek penting dari isu sumber daya alam, UU Desa juga hanya memberikan kewenangan yang minim terhadap swakelola sumber daya alam desa oleh pemerintah desa serta tidak menyentuh ketimpangan akses warga desa terhadap sumber daya alam setempat. Dihadapkan pada tantangan struktural demikian, perjuangan "otonomi desa" akan sulit mendorong transformasi sosial yang berarti tanpa melibatkan upaya penataan sumber daya alam yang berkeadilan dan berkelanjutan. Pada saat yang sama, perjuangan "keadilan sosial-ekologis" akan sulit tampil sebagai agenda kolektif desa tanpa mengupayakan demokratisasi yang lebih dalam di internal desa sendiri. Tulisan ini menawarkan kerangka perjuangan "demokratisasi tata kelola sumber daya alam desa" sebagai konvergensi strategis dari dua perjuangan sebelumnya: "otonomi desa" dan "keadilan sosial-ekologis". Hal ini diupayakan melalui tiga agenda konkret yang saling terkait: penguatan kewenangan desa atas sumber daya alam setempat, demokratisasi relasi-relasi sosio-agraria di desa, dan pembalikan krisis pedesaan untuk merevitalisasi basis-basis produksi desa.Law Number 6 of 2014 on Village-apart from its political contribution in democratizing state-village relation-has a fundamental limitation on natural resource issues in the village in the light of agrarian and ecological crises. This Law offers a minor elaboration on natural resource issues and provides limited authority to the village on this field, while no reference is made to the problem of inequality in community's access to local natural resources. Confronted with such structural challenges, it is argued that "struggle for village autonomy" will

  7. Preparations of prokaryotic expression system, standard protein and antiserum, of human Y- box binding protein 1%人YB-1的高效原核表达及其标准蛋白与抗血清的制备

    Institute of Scientific and Technical Information of China (English)

    李朴; 史静; 郭变琴; 钟梁; 梁勤东; 涂植光

    2011-01-01

    Aim: To construct a GST- expression system of human Y - box binding protein 1 (YB - 1), prepare the YB - 1 standard protein and its antiserum. Methods : The code sequence of YB - 1 was subcloned to the expression vector pGEX - 6P - 1. The recombinant vector was transformed into E. coli BL21 to express fusion protein GST- YB1. SDS- PAGE was applied to analyze the expression level and form of fusion protein. Then, YB- 1 standard protein was obtained by the means of GST - affinity chromatography and column - protease - digestion. The rabbit was immunized with YB - 1 protein to prepare the anti - YB1 polyclonal antibody. Results: The recombinant expression vector was constructed. SDS - PAGE and Western blot results showed that the GST - fusion protein was high- level expressed with soluble - form, and YB - 1 standard protein and its antisermn were obtained successfully. Conclusions: An economical, rapid method to prepare YB - 1 standard protein is established, and further obtained the high titer and affinity YB - 1 polyclonal antibody, which lay foundations for preparation of YB - 1 monoclonal antibody and development of YB - 1 quantitative analysis methods.%目的:构建YB1-GST表达系统,建立经济高效YB-1蛋白制备方法并制备其多抗。方法:将YB-1编码序列亚克隆至表达载体pGEX-6P-1;转化表达菌并确定可溶性表达最佳条件;采用GST亲和层析与层析柱上PSP酶切融合蛋白获取无标签蛋白的YB-1,经超滤浓缩及Western blot鉴定后,进-步真空冷冻于燥,制备YB-1标准蛋白;采用大剂量YB-1长程免疫方案免疫家兔以制备其抗体。结果:成功构建了表达载体pGEX—YB1;SDS—PAGE结果显示,YB1-GST融合蛋白以可溶性表达为主;Western blot结果证实,表达产物经GST亲和层析、PSP酶切以及真空冷冻干燥后获得了纯度较高的YB-1标准蛋白,将该蛋白免疫家兔获得了较高效价与特异性的

  8. Screening of probiotics bacteria from coral reef using co-culture method for controlling vibriosis in tiger shrimp (Penaeus monodon larvae

    Directory of Open Access Journals (Sweden)

    Ade Dwi Sasanti

    2015-04-01

    Full Text Available ABSTRACTThis study was carried out to obtain bacteria isolates from coral reef using co-culture method which potentially inhibit Vibrio harveyi growth. A total of 110 isolates were isolated from Acropora sp., Merulina sp., Hystrix sp., Poecillophora sp., Porites sp., and Haliophora sp., and were screened for their antagonistic activity against V. harveyi in in vitro and in vivo test. Five candidate probiotics (5H1 candidate probiotics isolated from Acropora sp., 11I and 11G isolates isolated from Hystrix sp. and 13B and 13G1 isolates isolated from Poecillophora sp., was able to inhibit growth of V. harveyi MR5339 RFR up to 101‒102 cfu/mL. Two isolates (13B and 13G1 were not pathogenic at concentration 106 cfu/mL bacteria and could increase of survival rate of tiger shrimp (Penaeus monodon larvae in in vivo test. Survival rate of tiger shrimp larvae that treatment with 13B and 13G1 were 86,67% and 88,33%, and have a significant different with positive control (61,67%. Partial sequencing of 16S-rRNA showed that 13G1 isolate was similar to V. alginolyticus.Keywords: vibriosis, Vibrio harveyi, tiger shrimp, probiotic, coral reefABSTRAKPenelitian ini bertujuan untuk mendapatkan bakteri probiotik asal terumbu karang dengan metode kultur bersama untuk pengendalian penyakit vibriosis pada larva udang windu (Penaeus monodon. Sebanyak 110 isolat berhasil diisolasi dari Acropora sp., Merulina sp., Hystrix sp., Poecillophora sp., Porites sp., dan Heliophora sp. dan dilakukan penapisan untuk melihat aktivitas kemampuannya melawan Vibrio harveyi MR 5339 RfR dalam uji in vitro dan in vivo. Sebanyak 56 isolat menghasilkan daya hambat terhadap V. harveyi MR5339 RfR pada metode kultur bersama. Lima isolat kandidat probiotik (isolate 5H1 diisolasi dari Acropora sp., isolat 11I dan 11G diisolasi dari Hystrix sp., serta isolat 13B dan 13G1 yang diisolasi dari Poecillophora sp., mampu menekan pertumbuhan V. harveyi MR5339 RfR hingga 101–102 cfu/mL. Kedua isolat

  9. Prevention of White Spot Syndrome Virus Infection on Penaeus monodon by Immersion in CEPM Extract of Avicennia sp. and Sonneratia sp.

    Directory of Open Access Journals (Sweden)

    D. Wahjuningrum

    2007-01-01

    Full Text Available The quality and survival rate are still being the problem that hampers the productivity of black tiger shrimp, Penaeus monodon culture.  Impaired quality of larval shrimp and environmental conditions can confer shrimp be infected by diseases, including viruses such as white spot syndrome virus (WSSV.  Prevention of disease infection using chemicals can offer negative impacts on water, pathogen resistance and consumers.  This study was conducted to examine the efficacy of an alternative prevention compound as liquid mangrove extract (CEPM from Avicennia sp. and Sonneratia sp. By immersion in different dose of CEPM, i.e. 250, 500, 750 and 1000 ppm, the patogenicity of WSSV was found to be different.  Patogenicity of WSSV decreased after treatment by CEPM, hence this could be used to induce shrimp immunity.  Optimum dose of CEPM was 250 ppm, which could increased survival rate of shrimp after challenging by WSSV, up to 98.4% shrimp survived. Keywrods: WSSV, black tiger shrimp, extract, Avicennia sp., Sonneratia sp.   ABSTRAK Kualitas dan kelangsungan hidup merupakan masalah yang masih membatasi produktivitas budidaya udang windu Penaeus monodon. Kondisi udang dan kualitas lingkungan yang kurang baik dapat menyebabkan udang terserang penyakit, termasuk yang disebabkan oleh virus termasuk white spot syndrome virus (WSSV. Upaya pengendaliannya menggunakan bahan kimia secara berlebih dapat menimbulkan dampak negatif bagi lingkungan perairan, kesehatan konsumen dan menimbulkan resistensi patogen. Penelitian ini dilakukan untuk mengetahui efektivitas bahan alternatif berupa cairan ekstrak pohon mangrove (CEPM dari jenis Avicennia sp. dan Sonneratia sp. sebagai upaya pencegahan. Dengan perendaman beberapa konsentrasi yang berbeda (250, 500, 750 dan 1000 ppm penggunaan cairan ekstrak pohon mangrove (CEPM Avicennia sp. dan Sonneratia sp., memberikan pengaruh yang berbeda terhadap patogenitas WSSV dan udang uji pada setiap perlakuan. Tingkat

  10. Application of Spirulina platensis for prevention of white spot syndrome virus in post larvae and juvenile black tiger shrimp (Penaeus monodon

    Directory of Open Access Journals (Sweden)

    Hemtanon, P.

    2005-02-01

    Full Text Available In this study, the extract of Spirulina platensis were examined in vitro to inhibit white spot syndrome virus (WSSV and application of dry S. platensis in diet for prevention of white spot syndrome (WSS in post larvae and juvenile black tiger shrimp (Penaeus monodon. The results showed that the lowest concentration of the extract for inhibiting WSSV was 0.01 mg/ml, while the optimum concentration was found to be 0.1 mg/ ml in which the mortality rate of the shrimp was 4 percents and infection was not detected from survivalshrimp by the immunohistochemistry method.Furthermore, The results showed that the survival rate of the post larvae fed on steamed egg containing dry S. platensis 5 g/kg of diet was higher than that of the control (p<0.05 when challenged with WSSV and no WSSV infected shrimp examine by polymerase chain reaction (PCR assay. In the case of juvenile shrimp, the survival rate of shrimp fed pellets containing dry S. platensis 10 g/kg of diet was higher than that of the control group (p<0.05 after challenging with WSSV. Moreover percent of WSSV infection in the survival shrimp using the immunohistochemistry method was lower than that of the control group.

  11. MARINE SPONGE Aaptos suberitoides, IT’S POTENTIAL SOURCE OF NATURAL ANTIBACTERIAL FOR CONTROLLING Vibrio harveyi ON TIGER SHRIMP (Penaeus monodon CULTURE

    Directory of Open Access Journals (Sweden)

    Rosmiati Rosmiati

    2015-06-01

    Full Text Available The study aims to isolate and identify the natural antibacterial compounds potential from Aaptos suberitoides for Vibrio harveyi control on tiger shrimp (Penaeus monodon culture. The agar diffusion method using paper discs was used to determine the antibacterial activity of extracts (diethyl ether (DEE, butanol (BUE and aqueous (HOE and compounds successfully isolated against Vibrio harveyi. Findings showed that the antibacterial activity was concentrated in BUE with the inhibition zone of 17.2±0.1 mm. Meanwhile, two other extracts (DEE and HOE did not exhibit any antibacterial activity against V. harveyi. From the active BUE, it was successfully isolated two compounds giving a strong anti-vibrio activity with the inhibition zone of 22±0.1 mm. The IR, 1H, 13C, COSY, HMQC, HMBC, and MS spectrum analysis indicated that both active compounds identified as aaptamine (1 and 9-demethyloxyaaptamine (2. The study suggested that marine sponge A. suberitoides may have potential compounds source for controlling of V. harveyi on tiger shrimp culture.

  12. Expression of Toll-like receptors (TLR, in lymphoid organ of black tiger shrimp (Penaeus monodon in response to Vibrio harveyi infection

    Directory of Open Access Journals (Sweden)

    Mundanda Muthappa Dechamma

    2015-05-01

    Full Text Available The Toll-like receptors (TLR, being pattern recognition molecules, are a powerful first line of defense in response to pathogen invasion. They are known to play a crucial role in detecting and binding to the microbial molecule and triggering a non-specific immune response. Quantitative real time PCR (qPCR expression of the TLR gene was studied in healthy and Vibrio harveyi infected black tiger shrimp (Penaeus monodon. Lymphoid tissue expression of TLR in V. harveyi infected animals 24 h post injection showed statistically significant up regulation of the gene as compared to the control animals sham injected with phosphate buffered saline (PBS. The qPCR expression pattern of TLR at different time points in shrimp administered with the immunostimulant glucan for 6 days by oral feeding followed by challenge with V. harveyi showed statistically significant level at 48 h post bacterial challenge as compared to the control (immunostimulant treated animals sham injected with PBS. The novelty of the study is that it elicits the role of TLRs as important response proteins of the innate immune system in the shrimp.

  13. Model Polikultur Udang Windu (Penaeus monodon Fab, Ikan Bandeng (Chanos-chanos Forskal dan Rumput Laut (Gracillaria Sp. Secara Tradisional

    Directory of Open Access Journals (Sweden)

    Murachman - -

    2012-05-01

    Full Text Available Abstrak Polikultur merupakan salah satu alternatif untuk mengatasi permasalahan air yang mengakibatkan penurunan produksi ikan di kolam. Penelitian ini bertujuan untuk mengetahui menyusun pola manajemen polikultur udang windu (Penaeus monodon Fab., ikan bandeng (Chanos-chanos Forskal dan rumput laut (Gracillaria sp.. Penelitian dilakukan dengan metode studi kasus di Dusun Tanjung Sari, Desa Kupang, Kecamatan Jabon, Kabupaten Sidoarjo. Sampel penelitian diambil pada 18 lokasi polikultur dari tiga komoditas tersebut (udang windu, ikan bandeng dan rumput laut dan 20 lokasi polikultur dari dua komunitas (udang windu dan ikan bandeng. Variabel yang dipergunakan pada penelitian ini adalah Variabel penelitian meliputi lingkungan makro tambak, karakteristik pembudidaya, cara pengelolaan tambak dan perlakuan–perlakuan yang diberikan, padat tebar, kualitas air, kesuburan air, produksi tambak, keuntungan pembudidaya polikultur dan model budidaya polikultur tiga komoditas. Model budidaya polikultur terdiri dari enam komponen yaitu penentuan lokasi tambak, persiapan tambak, pemeliharaan, panen, kelembagaan sosial dan kelembagaan ekonomi. Masing-masing komponne tersebut saling berhubungan. Berdasarkan hasil penelitian diketahui bahwa terdapat tiga faktor yang mendukung penentuan lokasi kolam, yaitu jenis tanah di atas kolam, sumber air tawar, sumber air laut, dan keberadaan hutan mangrove. Kualitas dan kesuburan air cukup baik dan berada pada kisaran standard kualitas air untuk tambak. Tidak terdapat perbedaan yang signifikan antara padat tebar untuk udang windu dan ikan bandeng pada tipe polikultur tiga komoditas dan polikultur dua komoditas. Padat tebar rumput laut pada polikultur tiga komoditas adalah 975 kgha-1. Keberadaan rumput laut pada polikultur tiga komoditas dapat meningkatkan kualitas air menjadi lebih baik dibandingkan pada polikultur dua komoditas. Kandungan oksigen terlarut pada tambak adalah 165 mgl-1, tingkat kejernihan air 50.875 cm, NH3 0

  14. Comparison of Penaeus monodon (Crustacea,Penaeidae) growth between commercial feed vscommercial shrimp feed supplemented with Kappaphycus alvarezii (Rhodophyta, Solieriaceae)seaweed sap

    OpenAIRE

    Kotiya Anil S.; Gunalan Balakrishanan; Jetani Kanji L.; Solanki Jitesh B.; Ramchandran Kumaran

    2011-01-01

    The consumer of seafood relates the potential development of animals to the constant use of commercial growth promoters. Nevertheless, nowadays with the development of non antibiotic feed additives, cultured shrimps performance has improved to great level. Present experiment was carried conducted from 27th May 2009 to 11th October 2009 in shrimp grow out ponds at Diu (U.T) region of Saurashtra coast. The stocking density of tiger shrimp Penaeus monodon Fabricius, 1798 was 7.5 no/m2 in both th...

  15. ANALISIS TATA KELOLA OPTIMALISASI SUMBER DAYA SISTEM INFORMASI MANAJEMEN JEMBATAN TIMBANG (EDM04 BERDASARKAN KERANGKA KERJA COBIT 5 PADA DINAS PERHUBUNGAN KOMUNIKASI DAN INFORMATIKA PROVINSI JAWA TENGAH

    Directory of Open Access Journals (Sweden)

    Yudistira Dian Hastiti

    2016-06-01

    Full Text Available Dinas Perhubungan Komunikasi dan Informatika (Dinhubkominfo Provinsi Jawa tengah sebagai perumus dan pelaksana kebijakan teknis, fasilitator, dan evaluator terkait penyelengaraan kegiatan penimbangan kendaraan bermotor melalui 16 jembatan timbang yang tersebar di wilayah provinsi jawa tengah telah mengimplementasikan sebuah system yang dipergunakan untuk kemudahan dalam menimbang kendaraan dengan mendata setiap kendaraan pada suatu system yang disebut Sistem Informasi Manajemen Jembatan Timbang (SIM JT. Masalah yang ditemukan saat ini yaitu, melihat padatnya antrian panjang kendaraan yang akan ditimbang disebabkan karena SIM JT yang belum memiliki database identitas kendaraan dan tidak dapat terpantau secara real time yang terjadi saat local server dalam keadaan down. Berdasarkan hal tersebut Dinhubkominfo berupaya mengoptimalkan kinerja SIM JT baik dari segi sumber daya manajemen manusia, TI, serta keuangan untuk meminimalkan kesalahan serta meningkatkan efektifitas pelayanan. Dari hasil studi dokumen, wawancara, dan kuesioner  berdasarkan COBIT 5 menghasilkan tingkat kapabilitas tata kelola proses optimalisasi sumer daya (EDM04 pada Dinas Perhubungan Komunikasi dan Informatika Provinsi Jawa Tengah saat ini berada di level 3 dengan status pencapaian Largely Achieved sebesar 80,18% setara dengan 3,80 dimana level 0, 1, dan 2 mencapai status Fully Achieved. Hal ini menunjukan telah mengelola dengan baik proses optimalisasi sumber daya dan diimplementasikan untuk mendukung pengerjaan proses standar dan efektif. Untuk mencapai tingkat target, Dinhubkominfo dapat melakukan strategi perbaikan dengan memperhatikan secara bertahap dari proses atribut level 1 sampai 4 Kata Kunci: Analisis Tata Kelola TI, COBIT 5, Sistem Informasi Manajemen Jembatan Timbang, Analisis Tingkat Kapabilitas, Analisis Kesenjangan.

  16. Oxidative addition of diphenyl disulfide across a Ta=Ta bond. Preparation and characterization of [TaCl3(Me2S)]2(μ-SPh)2

    International Nuclear Information System (INIS)

    Campbell, G.C.; Canich, J.A.M; Cotton, F.A.; Duraj, S.A.; Haw, J.F.

    1986-01-01

    The tantalum complex (SMe 2 )Cl 2 Ta(μ-Cl) 2 (μ-SMe 2 )TaCl 2 (SMe 2 ), possessing a sigma 2 π 2 Ta=Ta double bond, reacts readily with PhSSPh to give (SMe 2 )Cl 3 Ta(μ-SPh) 2 TaCl 2 (SMe 2 ). In this reaction, the starting material loses the bridging SMe 2 ligand and two chloride bridges are broken while only two new SPh bridges are formed in the final product. This oxidative-addition reaction of the S-S single bond to the Ta=Ta double bond converts the face-sharing bioctahedron structure of the starting compound to an edge-sharing bioctahedron structure in the final dimer, with concomitant change of the oxidation state of tantalum from III to IV. The product is the first example of a d 1 -d 1 ditantalum thiolate-bridged dimer. Important structural data for (SMe 2 )Cl 3 Ta(μ-SPh) 2 TaCl 3 (SMe 2 ), which has an inversion center, are determined. The new compound crystallizes in the monoclinic space group C2/c with a = 17.934 (5) A, b = 12.445 (4) A, c = 11.705 (4) A, β = 92.50 (3) 0 , V = 2610 (2) A 3 , and Z = 4. Solid-state 13 C NMR spectroscopy with cross polarization and magic-angle spinning (CP/MAS) at 28 and -103 0 C provides evidence that this compound is diamagnetic. 12 references, 3 figures, 4 tables

  17. High-mobility group (HMG) protein HMG-1 and TATA-binding protein-associated factor TAF(II)30 affect estrogen receptor-mediated transcriptional activation.

    Science.gov (United States)

    Verrier, C S; Roodi, N; Yee, C J; Bailey, L R; Jensen, R A; Bustin, M; Parl, F F

    1997-07-01

    The estrogen receptor (ER) belongs to a family of ligand-inducible nuclear receptors that exert their effects by binding to cis-acting DNA elements in the regulatory region of target genes. The detailed mechanisms by which ER interacts with the estrogen response element (ERE) and affects transcription still remain to be elucidated. To study the ER-ERE interaction and transcription initiation, we employed purified recombinant ER expressed in both the baculovirus-Sf9 and his-tagged bacterial systems. The effect of high-mobility group (HMG) protein HMG-1 and purified recombinant TATA-binding protein-associated factor TAF(II)30 on ER-ERE binding and transcription initiation were assessed by electrophoretic mobility shift assay and in vitro transcription from an ERE-containing template (pERE2LovTATA), respectively. We find that purified, recombinant ER fails to bind to ERE in spite of high ligand-binding activity and electrophoretic and immunological properties identical to ER in MCF-7 breast cancer cells. HMG-1 interacts with ER and promotes ER-ERE binding in a concentration- and time-dependent manner. The effectiveness of HMG-1 to stimulate ER-ERE binding in the electrophoretic mobility shift assay depends on the sequence flanking the ERE consensus as well as the position of the latter in the oligonucleotide. We find that TAF(II)30 has no effect on ER-ERE binding either alone or in combination with ER and HMG-1. Although HMG-1 promotes ER-ERE binding, it fails to stimulate transcription initiation either in the presence or absence of hormone. In contrast, TAF(II)30, while not affecting ER-ERE binding, stimulates transcription initiation 20-fold in the presence of HMG-1. These results indicate that HMG-1 and TAF(II)30 act in sequence, the former acting to promote ER-ERE binding followed by the latter to stimulate transcription initiation.

  18. Effect of molases addition on survival and growth of tiger shrimp Penaeus monodon Fab. larva treated with SKT-b Vibrio probiotic bacteria

    Directory of Open Access Journals (Sweden)

    , Widanarni

    2015-03-01

    Full Text Available  ABSTRACTProbiotic bacteria SKT-b Vibrio that belong to heterotrophic bacteria have been tested can suppress the growth of pathogenic Vibrio harveyi in tiger shrimp larvae. One of the most important energy sources of heterotrophic bacteria is organic carbon such as sucrose and glucose found in molasses. The objective of this experiment was to study the effect of molasses addition on the survival and growth rate of tiger shrimp Penaeus monodon larvae treated with SKT-b Vibrio probiotic bacteria. This experiment was done both in vitro and in vivo. In vitro tests performed by growing bacteria in media containing molasses, fish meal and premix. Whereas in vivo tests performed with the addition of molasses to shrimp larva culture media inoculated with SKT-b Vibrio bacteria which included five treatments: the control treatment without bacteria and molasses addition (K, the addition of bacteria without molasses (B, the addition of molasses 1 ppm and bacteria (MB1, the addition of molasses 3 ppm and bacteria (MB3, and the addition of molasses 5 ppm and bacteria (MB5. The result of in vitro tests showed the SKT-b Vibrio bacteria can grow on molasses media with a population reached 0,41 × 108 CFU/ml. In vivo test results showed that treatment MB3 gave the highest survival (93.3% and growth weight (35.94%, but were not significantly different from control result in the survival and growth weight that respectively were 83.3% and 30.38%.Keywords: probiotic bacteria, SKT-b Vibrio, molasses, tiger shrimp larvae ABSTRAKBakteri probiotik Vibrio SKT-b yang tergolong bakteri heterotrof telah diuji dapat menekan pertumbuhan Vibrio harveyi yang bersifat patogen pada larva udang windu. Salah satu sumber energi yang paling penting bagi bakteri heterotrof adalah karbon organik seperti sukrosa dan glukosa yang terdapat pada molase. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh penambahan molase terhadap kelangsungan hidup dan pertumbuhan larva udang

  19. Immunostimulation and yellow head virus (YHV) disease resistance induced by a lignin-based pulping by-product in black tiger shrimp (Penaeus monodon Linn.).

    Science.gov (United States)

    Srisapoome, Prapansak; Hamano, Kaoru; Tsutsui, Isao; Iiyama, Kenji

    2018-01-01

    Yellow head virus (YHV) is classified as one of the most serious pathogens causing a harmful disease in many penaeids, especially black tiger shrimp (Penaeus monodon), with high economic loss. To determine a potent and practical prophylactic strategy for controlling this disease, the toxicity of the by-product kraft lignin and its ability to control severe YHV infection were investigated in juvenile black tiger shrimp (15.9 ± 1.2 g body weight). The median lethal dosage at 96 h (96-hrs LD 50 ) of lignin in shrimp was 297 mg/L. Lignin was further added to shrimp diets via top-dressing to assess its ability to elicit immune stimulation activity. At 14 days after feeding, shrimp fed 1, 3, 5 and 10 g of lignin/kg of diet exhibited significantly higher levels of phagocytic activity (PA) than the control group (P  0.05). Additionally, lignin supplementation at 1-10 g/kg for 14 days failed to protect experimental shrimp against YHV infection. The antiviral activity of lignin against YHV in black tiger shrimp was notable in vitro because compared to control shrimp (96.7 ± 5.8%; P by-product kraft lignin efficiently inhibits YHV infection in black tiger shrimp. This information will facilitate the development of practical methods to control yellow head disease in the marine shrimp culture industry. Copyright © 2017 Elsevier Ltd. All rights reserved.

  20. Type I and type II crustins from Penaeus monodon, genetic variation and antimicrobial activity of the most abundant crustinPm4.

    Science.gov (United States)

    Donpudsa, Suchao; Visetnan, Suwattana; Supungul, Premruethai; Tang, Sureerat; Tassanakajon, Anchalee; Rimphanitchayakit, Vichien

    2014-11-01

    An antimicrobial protein, crustin, is involved in the innate immunity of crustacean by defending the host directly against the microbial pathogens. By data mining the Penaeus monodon EST database, two type I crustins, carcininPm1 and 2, and ten type II crustins, crustinPm1-10, were identified. The abundant crustins were crustinPm1, 4 and 7, each with variation in the length of Gly-rich repeat among its members. A few crustinPm1, 4 and 7 with deletion in the Cys-rich region were also observed. Furthermore, the crustinPm4 with the longest N-terminal Gly-rich region was characterized. The crustinPm4 allelic genes were expressed mainly from the hemocytes. Its expression was up-regulated readily by WSSV infection and gradually decreased to normal level afterwards. The recombinant crustinPm4-1 (rcrustinPm4-1) isoform was produced using the Escherichia coli expression system and tested for its antimicrobial activity. The rcrustinPm4-1 was able to inhibit the growth of a Gram-positive bacterium, Bacillus megaterium but not Bacillus subtilis, Micrococcus luteus and Staphylococcus aureus. It also inhibited the growth of two Gram-negative bacteria, E. coli 363 and Vibrio harveyi 639 at lower potency. The rcrustinPm4-1 affected the WSSV infection because the expression of an intermediate early gene ie1 in WSSV-infected hemocyte cell culture was reduced. It was shown further that the rcrustinPm4-1 could delay by about one and a half days the manifestation of disease by WSSV. Copyright © 2014 Elsevier Ltd. All rights reserved.

  1. Effects of carotenoid sources on growth performance, blood parameters, disease resistance and stress tolerance in black tiger shrimp (Penaeus monodon Fabricius

    Directory of Open Access Journals (Sweden)

    Supamattaya, K.

    2005-02-01

    Full Text Available Two feeding trial were conducted to determine the effects of various sources of carotenoid on growth performance, disease resistance, blood parameters, stress tolerance and pigmentation in juvenile black tiger shrimp (Penaeus monodon. Trial I was performed in small shrimp (1 g average body weight. The shrimp were fed with control diet without carotenoid (diet 1 while diets 2 to 6 contained 50 mg/kg astaxanthin (Lucanthin Pink®, 125 mg/kg β-carotene (Lucarotin®, 200 mg/kg β-carotene (Lucarotin®, 125 mg/kg Betatene® extracted from Dunaliella and 3% dried Spirulina respectively. There was an improvement in color in all groups of shrimp fed caroteniod supplemented diets, but no significant differences in weight gain or survival among the shrimps fed each test diet (p>0.05. Resistance to white spot syndrome virus (WSSV infection and stress tolerance (salinity stress, were not significantly different among treatments. Trial II was performed in juvenile shrimp (10 g average body weight fed test diets containing 100 ppm astaxanthin (Lucanthin pink®, 125 mg/kg β-carotene (Lucarotin®, 250 mg/kg β-carotene (Lucarotin®, 250 mg/kg Betatene® and 3% dried Spirulina compared with those fed control diet without carotenoid. At the end of 6 weeks feeding period, shrimp fed control diet as well as astaxanthin and dried Spirulina supplemented diets had higher levels of total hemocyte counts than those of all β-carotene supplemented diets feeding group. However, phenoloxidase activity and clearance of pathogenic vibrio from the hemolymphwere not significantly different among the treatments (p>0.05. Astaxanthin levels were highest in the shrimp fed all carotenoid-supplemented diets. In conclusion, a natural carotenoid i.e. dried Spirulina and carotenoid extracted from Dunaliella which have a lower production cost than analytical carotenoid showed beneficial effects on shrimp feed supplement.

  2. Transcription factor IID in the Archaea: sequences in the Thermococcus celer genome would encode a product closely related to the TATA-binding protein of eukaryotes

    Science.gov (United States)

    Marsh, T. L.; Reich, C. I.; Whitelock, R. B.; Olsen, G. J.; Woese, C. R. (Principal Investigator)

    1994-01-01

    The first step in transcription initiation in eukaryotes is mediated by the TATA-binding protein, a subunit of the transcription factor IID complex. We have cloned and sequenced the gene for a presumptive homolog of this eukaryotic protein from Thermococcus celer, a member of the Archaea (formerly archaebacteria). The protein encoded by the archaeal gene is a tandem repeat of a conserved domain, corresponding to the repeated domain in its eukaryotic counterparts. Molecular phylogenetic analyses of the two halves of the repeat are consistent with the duplication occurring before the divergence of the archael and eukaryotic domains. In conjunction with previous observations of similarity in RNA polymerase subunit composition and sequences and the finding of a transcription factor IIB-like sequence in Pyrococcus woesei (a relative of T. celer) it appears that major features of the eukaryotic transcription apparatus were well-established before the origin of eukaryotic cellular organization. The divergence between the two halves of the archael protein is less than that between the halves of the individual eukaryotic sequences, indicating that the average rate of sequence change in the archael protein has been less than in its eukaryotic counterparts. To the extent that this lower rate applies to the genome as a whole, a clearer picture of the early genes (and gene families) that gave rise to present-day genomes is more apt to emerge from the study of sequences from the Archaea than from the corresponding sequences from eukaryotes.

  3. Analisa Ketelitian Geometrik Citra Pleiades 1A dan Worldview-2 untuk Pembuatan Peta Dasar Rencana Detail Tata Ruang Perkotaan (Studi Kasus: Surabaya Pusat

    Directory of Open Access Journals (Sweden)

    Ricko Buana Surya

    2017-01-01

    Full Text Available Perkembangan teknologi penginderaan jauh mulai banyak diterapkan untuk berbagai keperluan, misalnya pembuatan peta Rencana Detail Tata Ruang (RDTR dengan skala 1:5000. Saat ini, ada beberapa satelit yang dapat menghasilkan citra resolusi tinggi dengan resolusi spasial kurang dari 1-2 m. Namun data citra yang diperoleh dari satelit-satelit tersebut harus diolah sedemikian rupa untuk meminimalisasi adanya kesalahan, terutama kesalahan geometrik. Data citra Pleiades 1A tahun 2015 dan citra Worldview-2 tahun 2012. Kedua citra tersebut dikoreksi secara geometrik menggunakan dua metode transformasi yaitu affine dan polynomial orde-2. Proses koreksi geometrik dengan menggunakan 8 titik kontrol tanah (GCP tersebar merata pada keseluruhan area studi yaitu Surabaya Pusat. Koordinat GCP diukur langsung di lapangan menggunakan GPS geodetik dengan metode statik dan lama pengamatan untuk setiap titik sekitar 40 menit. Kemudian citra terkoreksi dilakukan uji ketelitian koordinat dengan menggunakan ICP sebanyak 12 titik. Hasil penelitian ini menunjukkan bahwa nilai RMSE menggunakan metode transformasi polynomial orde-2 lebih baik dibandingkan dengan metode affine. Citra Worldview-2 menghasilkan nilai RMSE lebih kecil dibandingkan dengan citra Pleiades 1A pada kedua metode transformasi yang digunakan. Untuk kelayakan citra sebagai peta dasar, citra Pleiades 1A memenuhi syarat peta skala 1:5000 dan citra Worldview-2 memenuhi syarat peta skala 1:5000.

  4. Studies on the immunomodulatory effect of polysaccharide gel extracted from Durio zibethinus in Penaeus monodon shrimp against Vibrio harveyi and WSSV.

    Science.gov (United States)

    Pholdaeng, Komsil; Pongsamart, Sunanta

    2010-04-01

    Oral administration of polysaccharide gel (PG) in shrimp diets revealed immunostimulating potential and disease resistance in Penaeus monodon (black tiger shrimp). PG from the fruit-rind of Durio zibethinus has been characterized to be a pectic polysaccharide with immunomodulating and antibacterial activities. PG inhibited growth of the shrimp bacterial pathogen, Vibrio harveyi 1526, by agar diffusion and broth microdilution tests. Clear inhibition zones on agar plates were observed at the lowest PG concentration of 3.1 mg/ml, where minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values for PG were 6.3 and 12.5 mg/ml, respectively. Each group of juvenile shrimps, initial mean body weight 0.29 +/- 0.04 g, was housed in a closed-recirculating treated water system and was fed with PG-supplemented diets containing 1, 2 and 3% PG or shrimp basal diet in the control group for 8 and 12 weeks. PG-supplemented diets did not contribute to the overall growth of black tiger shrimp. The immune response was evaluated by analysis of prophenoloxidase activity and total hemocyte count in the shrimp fed PG-supplemented diets for 12 weeks. Prophenoloxidase activity in shrimp fed the 1, 2 and 3% PG-supplemented diet and total hemocyte count in shrimp fed the 1 and 2% PG-supplemented diet were higher (P < 0.05) than those of the control group. The percent survival was higher in groups fed the 1-3% PG-supplemented diets in challenge tests with either white spot syndrome virus (WSSV) or the bacterium V. harveyi 1526 than that of the control group. Relative percent survival (RPS) values in groups fed the 2% PG-supplemented diet showed the highest RPS value for disease resistance of 100% (at Day 6) and 36% (at Day 4) in treated shrimp against viral and bacterial infection, respectively. Mortality of PG-supplemented diets in treated shrimps against WSSV infection was also found to be much lower (P < 0.05) than that of the control group. Copyright 2009

  5. Archaeal promoter architecture and mechanism of gene activation

    DEFF Research Database (Denmark)

    Peng, Nan; Ao, Xiang; Liang, Yun Xiang

    2011-01-01

    element named ara box directing arabinose-inducible expression and the basal promoter element TATA, serving as the binding site for the TATA-binding protein. Strikingly, these promoters possess a modular structure that allows an essentially inactive basal promoter to be strongly activated. The invoked...... mechanisms include TFB (transcription factor B) recruitment by the ara-box-binding factor to activate gene expression and modulation of TFB recruitment efficiency to yield differential gene expression....

  6. Evidence for multiple major histocompatibility class II X-box binding proteins.

    OpenAIRE

    Celada, A; Maki, R

    1989-01-01

    The X box is a loosely conserved DNA sequence that is located upstream of all major histocompatibility class II genes and is one of the cis-acting regulatory elements. Despite the similarity between all X-box sequences, each promoter-proximal X box in the mouse appears to bind a separate nuclear factor.

  7. Reference: 439 [Arabidopsis Phenome Database[Archive

    Lifescience Database Archive (English)

    Full Text Available or IID (TFIID) complex. Overexpression of atTAF10 under the control of the 35S promoter in Arabidopsis impro...is TATA box-binding protein (TBP)-associated factor 10 (atTAF10), which constitutes the transcriptional fact

  8. Cisplatin- and UV-damaged DNA lure the basal transcription factor TFIID/TBP.

    NARCIS (Netherlands)

    P. Vichi; F. Coin (Frédéric); J-P. Renaud (Jean-Paul); W. Vermeulen (Wim); J.H.J. Hoeijmakers (Jan); D. Moras; J-M. Egly (Jean-Marc)

    1997-01-01

    textabstractA connection between transcription and DNA repair was demonstrated previously through the characterization of TFIIH. Using filter binding as well as in vitro transcription challenge competition assays, we now show that the promoter recognition factor TATA box-binding protein (TBP)/TFIID

  9. B1-induced caspase-independent apoptosis in MCF-7 cells is mediated by down-regulation of Bcl-2 via p53 binding to P2 promoter TATA box

    International Nuclear Information System (INIS)

    Liang Xin; Xu Ke; Xu Yufang; Liu Jianwen; Qian Xuhong

    2011-01-01

    The Bcl-2 family contains a panel of proteins which are conserved regulators of apoptosis in mammalian cells, like the anti-apoptotic protein Bcl-2. According to its significant role in altering susceptibility to apoptosis, the deciphering of the mechanism of Bcl-2 expression modulation may be crucial for identifying therapeutics strategies for cancer. Treatment with naphthalimide-based DNA intercalators, including M2-A and R16, generally leads to a decrease in Bcl-2 intracellular amounts. Whereas the interest for these chemotherapeutics is accompanied by advances in the fundamental understanding of their anticancer properties, the molecular mechanism underlying changes in Bcl-2 expression remains poorly understood. We report here that p53 contributes to Bcl-2 down-regulation induced by B1, a novel naphthalimide-based DNA intercalating agent. Indeed, the decrease in Bcl-2 protein levels observed during B1-induced apoptosis was correlated to the decrease in mRNA levels, as a result of the inhibition of Bcl-2 transcription and promoter activity. In this context, we evaluated p53 contribution in the Bcl-2 transcriptional down-regulation. We found a significant increase of p53 binding to P 2 promoter TATA box in MCF7 cells by chromatin immunoprecipitation. These data suggest that B1-induced caspase-independent apoptosis in MCF-7 cells is associated with the activation of p53 and the down-regulation of Bcl-2. Our study strengthens the links between p53 and Bcl-2 at a transcriptional level, upon naphthalimide-based DNA intercalator treatment. - Research highlights: → B1 induced apoptosis in MCF-7 cells, following a transcriptional decrease in Bcl-2. → B1 treatment triggered p53 activation and leads to a p53-dependent down-regulation of Bcl-2. → B1 induced significant increase of p53 binding to Bcl-2 P 2 promoter TATA box.

  10. Candidate SNP markers of aggressiveness-related complications and comorbidities of genetic diseases are predicted by a significant change in the affinity of TATA-binding protein for human gene promoters.

    Science.gov (United States)

    Chadaeva, Irina V; Ponomarenko, Mikhail P; Rasskazov, Dmitry A; Sharypova, Ekaterina B; Kashina, Elena V; Matveeva, Marina Yu; Arshinova, Tatjana V; Ponomarenko, Petr M; Arkova, Olga V; Bondar, Natalia P; Savinkova, Ludmila K; Kolchanov, Nikolay A

    2016-12-28

    Aggressiveness in humans is a hereditary behavioral trait that mobilizes all systems of the body-first of all, the nervous and endocrine systems, and then the respiratory, vascular, muscular, and others-e.g., for the defense of oneself, children, family, shelter, territory, and other possessions as well as personal interests. The level of aggressiveness of a person determines many other characteristics of quality of life and lifespan, acting as a stress factor. Aggressive behavior depends on many parameters such as age, gender, diseases and treatment, diet, and environmental conditions. Among them, genetic factors are believed to be the main parameters that are well-studied at the factual level, but in actuality, genome-wide studies of aggressive behavior appeared relatively recently. One of the biggest projects of the modern science-1000 Genomes-involves identification of single nucleotide polymorphisms (SNPs), i.e., differences of individual genomes from the reference genome. SNPs can be associated with hereditary diseases, their complications, comorbidities, and responses to stress or a drug. Clinical comparisons between cohorts of patients and healthy volunteers (as a control) allow for identifying SNPs whose allele frequencies significantly separate them from one another as markers of the above conditions. Computer-based preliminary analysis of millions of SNPs detected by the 1000 Genomes project can accelerate clinical search for SNP markers due to preliminary whole-genome search for the most meaningful candidate SNP markers and discarding of neutral and poorly substantiated SNPs. Here, we combine two computer-based search methods for SNPs (that alter gene expression) {i} Web service SNP_TATA_Comparator (DNA sequence analysis) and {ii} PubMed-based manual search for articles on aggressiveness using heuristic keywords. Near the known binding sites for TATA-binding protein (TBP) in human gene promoters, we found aggressiveness-related candidate SNP markers

  11. CAROTENOID-ENRICHED DIET FOR PRE-MATURATION STAGE OF POND-REARED TIGER SHRIMP, Penaeus monodon PART I. THE EFFECTS ON GROWTH, PIGMENTATION AND WHOLE BODY NUTRIENT CONTENT

    Directory of Open Access Journals (Sweden)

    Asda Laining

    2017-12-01

    Full Text Available Carotenoids, besides as a natural pigment, may have vital roles in the growth of crustacean. The aim of this study was to clarify the influence of combined carotenoids given since pre-maturation stage on the growth performances, pigmentation and biochemical composition of the whole body of pond-reared tiger shrimp, Penaeus monodon. Two experimental diets were supplemented with or without carotenoid mixture consisting of astaxanthin, canthaxanthin and other carotenoids contained in Spirulina. The carotenoid mixture was supplemented in the commercial diet normally used as a starter feed for tiger shrimp, re-pelleted and fed to tiger shrimp with an initial body weight of 31.7±1.3 g. Shrimp were stocked in four 1,000 m2 concrete ponds with a density of 1 shrimp/m2 and fed until the shrimp reached maturation stage (broodstock size. Variables observed were growth performances and pigmentation properties during the pre-maturation stage and total carotenoid content in several tissues of the female broodstock after being fed with the diets until maturation stage. After 16 weeks, shrimp fed with carotenoid-enriched diet (PC diet produced significantly (P<0.05 higher biomass than the diet without the enrichment (PO. The color of raw shrimp fed with PC diet was darker with greenish-brown compared to shrimp fed PO diet which was greenish blue. The visual appearances of 3-min steamed shrimp produced the color of red-orange for shrimp fed carotenoid compared to orange-yellow for control PO. The total carotenoid content in the whole body of shrimp fed PC diet were significantly (P<0.05 enhanced compared to control PO diet which was 42.8 ±5.8 and 55.8± 5.1 µg/g for PO and PC diet, respectively. Supplemental carotenoid in the pre-maturation diet increased the biomass production from 23.1±1.9 kg to 30.2 ±0.1 kg and enhanced the color of the shrimp which was in line with carotenoid content in the whole body of pond-reared tiger shrimp.

  12. [open quotes]Cryptic[close quotes] repeating triplets of purines and pyrimidines (cRRY(i)) are frequent and polymorphic: Analysis of coding cRRY(i) in the proopiomelanocortin (POMC) and TATA-binding protein (TBP) genes

    Energy Technology Data Exchange (ETDEWEB)

    Gostout, B.; Qiang Liu; Sommer, S.S. (Mayo Clinic/Foundation, Rochester, MN (United States))

    1993-06-01

    Triplets of the form of purine, purine, pyrimidine (RRY(i)) are enhanced in frequency in the genomes of primates, rodents, and bacteria. Some RRY(i) are [open quotes]cryptic[close quotes] repeats (cRRY(i)) in which no one tandem run of a trinucleotide predominates. A search of human GenBank sequence revealed that the sequences of cRRY(i) are highly nonrandom. Three randomly chosen human cRRY(i) were sequenced in search of polymorphic alleles. Multiple polymorphic alleles were found in cRRY(i) in the coding regions of the genes for proopiomelanocortin (POMC) and TATA-binding protein (TBP). The highly polymorphic TBP cRRY(i) was characterized in detail. Direct sequencing of 157 unrelated human alleles demonstrated the presence of 20 different alleles which resulted in 29--40 consecutive glutamines in the amino-terminal region of TBP. These alleles are differently distributed among the races. PCR was used to screen 1,846 additional alleles in order to characterize more fully the range of variation in the population. Three additional alleles were discovered, but there was no example of a substantial sequence amplification as is seen in the repeat sequences associated with X-linked spinal and bulbar muscular atrophy, myotonic dystrophy, or the fragile-X syndrome. The structure of the TBP cRRY(i) is conserved in the five monkey species examined. In the chimpanzee, examination of four individuals revealed that the cRRY(i) was highly polymorphic, but the pattern of polymorphism differed from that in humans. The TBP cRRY(i) displays both similarities with and differences from the previously described RRY(i) in the coding sequence of the androgen receptor. The data suggest how simple tandem repeats could evolve from cryptic repeats. 18 refs., 3 figs., 6 tabs.

  13. Transcriptional autorepression of Msx1 gene is mediated by interactions of Msx1 protein with a multi-protein transcriptional complex containing TATA-binding protein, Sp1 and cAMP-response-element-binding protein-binding protein (CBP/p300).

    OpenAIRE

    Shetty, S; Takahashi, T; Matsui, H; Ayengar, R; Raghow, R

    1999-01-01

    The TATA-less murine Msx1 promoter contains two Msx1-binding motifs, located at -568 to -573 and +25 to +30, and is subject to potent autorepression [Takahashi, Guron, Shetty, Matsui and Raghow (1997) J. Biol. Chem. 272, 22667-22678]. To investigate the molecular mechanism by which Msx1 represses the activity of its own promoter, we transfected C2C12 myoblasts with Msx1-promoter-luciferase constructs and assessed reporter gene activity, with and without the exogenous expression of Msx1. We de...

  14. OPTIMASI TATA GUNA LAHAN DAN PENERAPAN REKAYASA TEKNIK DALAM ANALISA BANJIR DI DAERAH ALIRAN SUNGAI: STUDI KASUS DAERAH ALIRAN SUNGAI CILIWUNG HULU DI BENDUNG KATULAMPA (The Optimization of Land Use and the Application of Engineering Treatment in Flood

    Directory of Open Access Journals (Sweden)

    Yohana Lilis Handayani

    2005-07-01

    Full Text Available ABSTRAK Peningkatan aliran puncak dan volume runoff dari flood hydrograph dapat disebabkan oleh konversi penggunaan lahan. Fenomena ini terjadi di cekungan hulu sungai Ciliwung sebagai daerah konservasi. DAS ini memiliki peran penting dalam memelihara ketersediaan air di cekungan Ciliwung dan untuk pengendalian banjir di daerah hilir. Berdasarkan data yang dicatat dari 1993 sampai dengan 1996, 14,6% kejadian banjir di daerah hilir disebabkan oleh banjir kiriman. Evaluasi konversi penggunaan lahan di daerah hulu Ciliwung dilakukan dengan membandingkan penggunaan lahan tahun 1989 dan 1998. Optimasi tata guna lahan dilakukan dengan optimasi linier untuk meminimasi nilai koefisien composite runoff. Pendekatan teknis dan penerapan rekayasa teknik digunakan untuk simulasi penurunan aliran puncak dan volume runoff dari flood hydrograph. Perlakuan ini meliputi terracing dan normalisasi kolam detensi (detention pond. Simulasi dilakukan untuk periode banjir 10 tahunan. Hasil analisis menunjukkan bahwa dalam periode 10 tahun (1989-1998 penggunaan lahan dikonversi secara signifikan. Konversi ini menyebabkan peningkatan aliran puncak dan volume runoff masing-masing 18,97% dan 18,87%.   ABSTRACT The risk of peak flow and runoff volume of a flood hydrograph may be caused by land use conversion. This phenomenon had happened in upstream of Ciliwung basin. As a conversation area, this catchment has an important role in maintaining the water availability of Ciliwung basin and for flood control in downstream area. Based on the collected flood data recorded from 1993 to 1996, 14.6% of flood events in downstream of Ciliwung basin were caused by delivery flood from upstream area. Evaluation of land use conversion in upstream of Ciliwung basin was carried out by comparing land use in 1989 and in 1998. Land use optimization was done using linear optimization to minimize the value of composite runoff coefficient. Technical approach of engineering treatment was used to

  15. DESAIN VEGETASI BERNILAI KONSERVASI DAN EKONOMI PADA KAWASAN PENYANGGA SISTEM TATA AIR DAS BOLANGO (Designing of Vegetation which Conservation and Economic Values in the Buffer Area of Water System at the Bolango Watershed

    Directory of Open Access Journals (Sweden)

    Danang Wahyu Purnomo

    2016-02-01

    Full Text Available ABSTRAK Perencanaan pembangunan arboretum di DAS Bolango dengan konsep konservasi dan ekonomi perlu dilakukan karena DAS ini memiliki peranan yang penting dalam kehidupan masyarakat sekitar. Tujuan penelitian ini adalah memberikan rekomendasi tentang komposisi dan struktur vegetasi penyusun hutan pada kawasan arboretum sebagai pemelihara mata air Sungai Bolango. Penelitian dimulai dengan mengidentifikasi sumber mata air, tanah, dan kondisi vegetasi eksisting. Metode wawancara kepada masyarakat setempat dilakukan untuk mendukung data etnobotani. Kajian lahan dilakukan antara lain tata guna, kelas kemampuan, konsep pengelolaan, kesesuaian lahan, dan penentuan vegetasinya. Hasil identifikasi sumber mata air menunjukkan bahwa terdapat 3 lokasi yang potensial dibangun arboretum, yaitu Desa Meranti Kecamatan Tapa Kabupaten Bone Bolango, Desa Dulamayo Selatan Kecamatan Telaga Kabupaten Gorontalo, dan Desa Mongiilo Kecamatan Bolango Ulu Kabupaten Bone Bolango. Berdasarkan data kualitas tanah, ketiga lokasi memiliki media perakaran yang cukup baik untuk tanaman budi daya. Secara umum, semua jenis tanaman budi daya sesuai untuk ditanam di ketiga lokasi arboretum. Perlu dilakukan pembuatan teras dan penerapan pola tanam konservasi yang mengaplikasikan tanaman penutup tanah (cover crop, tanaman budi daya, dan pohon penyusun hutan. Selain itu, perlu pemberian pupuk organik berupa kompos dan pupuk kandang.   ABSTRACT Arboretum development planning in Bolango Watershed using concept of conservation and economy is conducted because the watershed has an important role in people's lives around. This study aims to provide recommendations about composition and structure of forest vegetation in the arboretum area for conserving of Bolango River’s water springs. The study began by identifying the source of the springs, soil, and the existing vegetation. Interview to local communities was conducted to support the data of ethnobotany. Land observation was studied

  16. DIAGNOSIS DAN TATA LAKSANA ENSEFALITIS HERPES SIMPLEKS

    Directory of Open Access Journals (Sweden)

    Tri Yuliantini

    2014-12-01

    Full Text Available Infeksi Herpes simpleks pada susunan saraf pusat (SSP merupakan infeksi SSP yang paling beratdan sering berakibat fatal. Angka kejadiannya diperkirakan 1 kasus per 250 000 sampai 500 000orang per tahun, sepertiganya terjadi pada anak-anak. Gejala dan tanda klinis pada fase awal sangattidak khas. Pemberian terapi yang terlambat membawa dampak terjadinya kecacatan permanen.Deteksi virus Herpes simpleks (VHS di dalam cairan serebrospinal dengan polymerase chain reactionmerupakan modalitas pilihan untuk diagnosis ensefalitis herpes simpleks (EHS. Asiklovir intravenamerupakan obat pilihan pertama. Pengobatan segera diberikan kepada pasien yang dicurigai menderitaEHS, kemudian pengobatan dapat dilanjutkan atau dihentikan sesuai konfirmasi laboratorium atauhasil biopsi otak. Pasien yang tidak diberikan antivirus atau pengobatannya terlambat angkakematiannya cukup tinggi.

  17. TATA BANGUNAN FASADE DI KORIDOR LEGIAN, KUTA

    Directory of Open Access Journals (Sweden)

    Widiastuti -

    2015-07-01

    Full Text Available This study, which takes place along Legian Corridor in Kuta-Bali, is conducted to achieve threemain goals. The first is to identify forms of facade exhibited by various structures existing alongthis Corridor. Second, to evaluate the conformance of buildings. The key legislation here is LocalRegulation No 5 year 2005 regarding Building Codes and Architectural Requirements. Third, inthe process to investigate inefficiencies in land use based on building heights. So this studyfocuses on basic building forms, architectural styles, building heights, and the use of buildingmaterials. Descriptive qualitative research methods are used, supported with photographicdocumentation of all the 129 units being surveyed. Research findings demonstrate that 62 % ofthese building units do not incorporate Balinese architectural elements as is required; 61 % usevarious fabricated building materials; and 65.8 % of the units are single storey structures. Theseconditions indicate violations of building codes and regulations enforced across the Island, as wellas inefficient land use within the Legian Corridor where land price is relatively very high.

  18. TATA KELOLA TEKNOLOGI INFORMASI (IT GOVERNANCE

    Directory of Open Access Journals (Sweden)

    Ita Ernala Kaban

    2009-05-01

    Full Text Available Technology advances offer many advantages for information and data storage, delivery, access and processing. Technology advances have also placed information as one of the most prominent resources requiring good and proper management. Information technology management in enterprise, although has been implemented, yet has not been managed using structured approach, thus, it is difficult to measure the roles of Information Technology in supporting business process for achieving enterprise objectives. In that way, the aim of this research is to analyze standard model for IT Governance. From the study, it can be concluded that COBIT has a better compromise in its horizontal and vertical dimension compared to the other standards. COBIT has a wider and more detailed IT process spectrum.Keywords: IT Governance, enterprise, business process

  19. Bank Indonesia dalam Tata Pemerintahan Indonesia

    OpenAIRE

    Ismail, Maqdir

    2010-01-01

    This research focuses on problems as follows: First, position and role of Bank Indonesia as a state body in Indonesia; Second, independency of central bank in performing its monetary function. This research is a juridical normative research using primary legal source which is legislation concerning central bank. Data is collected through document study and literary study. This research concludes that position of Bank Indonesia as a legal entity should have been included in the explanation of ...

  20. Bank Indonesia Dalam Tata Pemerintahan Indonesia

    OpenAIRE

    Ismail, Maqdir

    2010-01-01

    This research focuses on problems as follows: First, position and role of Bank Indonesia as a state body in Indonesia; Second, independency of central bank in performing its monetary function. This research is a juridical normative research using primary legal source which is legislation concerning central bank. Data is collected through document study and literary study. This research concludes that position of Bank Indonesia as a legal entity should have been included in the explanation of ...

  1. DETERMINAN EFISIENSI USAHA BERDASARKAN TATA KELOLA KORPORAT

    Directory of Open Access Journals (Sweden)

    Intan Lifinda Ayuning Putri

    2016-12-01

    Full Text Available Abstract: Business Efficiency Determinant Constructed by Corporate Governance and Business. This study aims to examine the effect of corporate governance on the technical efficiency moderated by ownership structure and capital structure used tobit regression model and hierarchical regression analysis. This research in measured by DEA, around 30% manufacturing companies are efficient in 2013-2015. The result shows that there is positive effect of corporate governance on the technical efficiency indentified by proportion of independent board of commissioner. It is not found that another proxy has an influence. The result also shows that ownership structure has effected on the relationship between corporate governace and technical efficiency while capital structure has no influence.

  2. Adipocyte spliced form of X-box-binding protein 1 promotes adiponectin multimerization and systemic glucose homeostasis

    NARCIS (Netherlands)

    Sha, H.; Yang, L.; Liu, M.; Xia, S.; Liu, Y.; Liu, F.; Kersten, A.H.; Qi, L.

    2014-01-01

    The physiological role of the spliced form of X-box–binding protein 1 (XBP1s), a key transcription factor of the endoplasmic reticulum (ER) stress response, in adipose tissue remains largely unknown. In this study, we show that overexpression of XBP1s promotes adiponectin multimerization in

  3. MicroRNA-141 inhibits migration of gastric cancer by targeting zinc finger E-box-binding homeobox 2.

    Science.gov (United States)

    Du, Ying; Wang, Lingfei; Wu, Honghai; Zhang, Yiyin; Wang, Kan; Wu, Dingting

    2015-09-01

    Human microRNA (miR)-141 is a member of the miR‑200 family, which has been reported to be downregulated in gastric cancer, and involved in the proliferation of gastric cancer cells. However, little is currently known regarding its role in the migration of gastric cancer. The present study investigated the function of miR‑141 in gastric cancer cell migration, and evaluated the contribution of zinc finger E‑box‑binding homeobox 1 and 2 (ZEB1/2) in miR‑141 mediated migration of gastric cancer cells. The expression levels of miR‑141 and its potential ZEB1/2 targets were examined by quantitative polymerase chain reaction (qPCR) and western blotting, respectively. The migration of SGC‑7901 and HGC‑27 gastric cancer cells, which had been transfected with an miRNA precursor, was examined by cell migration and wound healing assays. A luciferase activity assay was used to validate whether ZEB1/2 was a direct target of miR‑141. The results demonstrated that overexpression of miR‑141 markedly inhibited the migration of gastric cancer cells in vitro. Forced overexpression of miR‑141 significantly reduced the luciferase activity of the 3'‑untranslated region of ZEB2 in gastric cancer cells. Furthermore, the mRNA and protein expression levels of ZEB2 were reduced in cells overexpressing miR‑141, whereas the protein expression levels of E‑cadherin were increased. In gastric tumor samples the expression levels of ZEB2 were inversely correlated with the expression of miR‑141. These results suggest that miR‑141 may be involved in the inhibition of gastric cancer cell migration, and that ZEB2 is a target gene of miR-141.

  4. TAF9b (Formerly TAF9L) Is a Bona Fide TAF That Has Unique and Overlapping Roles with TAF9

    OpenAIRE

    Frontini, Mattia; Soutoglou, Evi; Argentini, Manuela; Bole-Feysot, Christine; Jost, Bernard; Scheer, Elisabeth; Tora, Làszlò

    2005-01-01

    TFIID plays a key role in transcription initiation of RNA polymerase II preinitiation complex assembly. TFIID is comprised of the TATA box binding protein (TBP) and 14 TBP-associated factors (TAFs). A second set of transcriptional regulatory multiprotein complexes containing TAFs has been described (called SAGA, TFTC, STAGA, and PCAF/GCN5). Using matrix-assisted laser desorption ionization mass spectrometry, we identified a novel TFTC subunit, human TAF9Like, encoded by a TAF9 paralogue gene....

  5. Les structures de la couverture Néoprotérozoïque terminal et Paléozoïque de la région de Tata, Anti-Atlas centre-occidental, Maroc: déformation polyphasée, ou interactions socle/couverture pendant l'orogenèse hercynienne?The structures of the Late Neoproterozoic and Early Palæozoic cover of the Tata area, western Anti-Atlas, Morocco: polyphased deformation or basement/cover interactions during the Variscan orogeny?

    Science.gov (United States)

    Faik, F.; Belfoul, M. A.; Bouabdelli, M.; Hassenforder, B.

    2001-05-01

    The western Anti-Atlas was formed by a Precambrian basement in the core of anticlines, surrounded by a Neoproterozoic and Palæozoic cover. The structural study of the Tata regional rocks shows a heterogeneous deformation, characterised especially by two types of folds in two orthogonal directions: north-south to north-northeast-south-southwest-trending and east-west-trending. The north-south structures are present in all of the Palæozoic cover and belong to the major Variscan compression of Late Carboniferous age by a comparison of the other domains of the western Anti-Atlas. Alternatively, east-west folding is assigned only to the lower part of the cover and consists of a ductile heterogeneous deformation, especially marked at the basement-cover interface. These folds are associated with a subhorizontal cleavage, indicating a southern vergence of the structures. A discussion of the age and the tectonic style of these structures is proposed, as well as their significance within the Variscan belt along the northern margin of the West African Craton.

  6. Y-box-binding protein-1 (YB-1) promotes cell proliferation, adhesion and drug resistance in diffuse large B-cell lymphoma

    Energy Technology Data Exchange (ETDEWEB)

    Miao, Xiaobing; Wu, Yaxun [Department of Pathology, Affiliated Cancer Hospital of Nantong University, Nantong 226361, Jiangsu (China); Wang, Yuchan [Department of Pathogen, Medical College, Nantong University, Nantong 226001, Jiangsu (China); Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong 226001, Jiangsu (China); Zhu, Xinghua; Yin, Haibing [Department of Pathology, Affiliated Cancer Hospital of Nantong University, Nantong 226361, Jiangsu (China); He, Yunhua [Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong 226001, Jiangsu (China); Li, Chunsun; Liu, Yushan; Lu, Xiaoyun; Chen, Yali; Shen, Rong [Department of Pathology, Affiliated Cancer Hospital of Nantong University, Nantong 226361, Jiangsu (China); Xu, Xiaohong, E-mail: xuxiaohongnantong@126.com [Department of Oncology, Affiliated Cancer Hospital of Nantong University, Nantong 226361, Jiangsu (China); He, Song, E-mail: hesongnt@126.com [Department of Pathology, Affiliated Cancer Hospital of Nantong University, Nantong 226361, Jiangsu (China)

    2016-08-15

    YB-1 is a multifunctional protein, which has been shown to correlate with resistance to treatment of various tumor types. This study investigated the expression and biologic function of YB-1 in diffuse large B-cell lymphoma (DLBCL). Immunohistochemical analysis showed that the expression statuses of YB-1 and pYB-1{sup S102} were reversely correlated with the clinical outcomes of DLBCL patients. In addition, we found that YB-1 could promote the proliferation of DLBCL cells by accelerating the G1/S transition. Ectopic expression of YB-1 could markedly increase the expression of cell cycle regulators cyclin D1 and cyclin E. Furthermore, we found that adhesion of DLBCL cells to fibronectin (FN) could increase YB-1 phosphorylation at Ser102 and pYB-1{sup S102} nuclear translocation. In addition, overexpression of YB-1 could increase the adhesion of DLBCL cells to FN. Intriguingly, we found that YB-1 overexpression could confer drug resistance through cell-adhesion dependent and independent mechanisms in DLBCL. Silencing of YB-1 could sensitize DLBCL cells to mitoxantrone and overcome cell adhesion-mediated drug resistance (CAM-DR) phenotype in an AKT-dependent manner. - Highlights: • The expression statuses of YB-1 and pYB-1{sup S102} are reversely correlated with outcomes of DLBCL patients. • YB-1 promotes cell proliferation by accelerating G1/S transition in DLBCL. • YB-1 confers drug resistance to mitoxantrone in DLBCL.

  7. Selection of reference genes for gene expression studies in pig tissues using SYBR green qPCR

    DEFF Research Database (Denmark)

    Hillig, Ann-Britt Nygaard; Jørgensen, Claus Bøttcher; Cirera, Susanna

    2007-01-01

    -microglobulin (B2M), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), hydroxymethylbilane synthase (HMBS), hypoxanthine phosphoribosyltransferase I (HPRT I), ribosomal protein L4 (RPL4), succinate dehydrogenase complex subunit A (SDHA), TATA box binding protein (TPB) and tyrosine 3-monooxygenase/tryptophan 5......-monooxygenase activation protein zeta polypeptide (YWHAZ). The stability of these reference genes in different pig tissues was investigated using the geNorm application. The range of expression stability in the genes analysed was (from the most stable to the least stable): ACTB/RPL4, TBP, HPRT, HMBS, YWHAZ...

  8. Complete regeneration of ablated eyestalk in penaeid prawn, Penaeus monodon

    Digital Repository Service at National Institute of Oceanography (India)

    Desai, U.M.; Achuthankutty, C

    regenerated in less than 6 months and assumed the shape, size, structure and pigmentation of the unablated eye. Significance of this observation in the context of captive broodstock development and the need for detailed examination of the regeneration process...

  9. Levels of PAHs in shrimps, Penaeus monodon from Jones Creek ...

    African Journals Online (AJOL)

    High concentrations were recorded during the rainy season and during the first quarter of the year. The investigated samples were classified as minimally contaminated when compared with FDA levels of PAHs in shrimp samples. Distribution patterns showed that PAHs with 3 and 4 rings dominated confirming the pyrogenic ...

  10. IMPROVED PRODUCTION OF TIGER SHRIMP (Penaeus monodon THROUGH PROBIOTICS APPLICATION

    Directory of Open Access Journals (Sweden)

    Irsyaphiani Insan

    2015-12-01

    Full Text Available The study was carried out in Brebes District, the North coast of Java. Tiger shrimp farming in Indonesia, particularly in this area faced some problems which caused by improper pond preparation, disease, and low seed quality. Probiotic was applied in pond to solve this problem. The aim of this study was to evaluate the production of tiger shrimp in ponds with probiotic applications. Six experimental ponds (each measuring 0.5 ha were selected of which three were probiotic ponds and three were controlled. Tiger shrimp postlarvae (PL-30 were stocked at density of four shrimps/m2. Tiger shrimps were reared for three months. Shrimps were fed by commercial pellet. In the first month, shrimp were fed about 7%-5% of the total biomass; in the second months, 3.5%-3% of the total biomass; and in the third month, 2.5%-2% of the total biomass. The treatments in this study were the application of probiotics with concentration of 3 mg/L that were given every five days and control (without probiotics. The results showed the rearing period was 92 ± 6 days in probiotic ponds and 76 ± 16 days in controlled pond. The shrimp in controlled pond should be harvest earlier caused by the high mortality. The average final weight was 16.2 ± 0.7 g in probiotic pond and 15.6 ± 1.9 g in controlled pond. The survival rate was 64.13 ± 12.63% in probiotic pond and 44.17 ± 14.15% in controlled pond. Production was 208 ± 46 kg/pond/cycle in probiotic pond and 123 ± 6 kg/pond/cycle in controlled pond. The result showed that probiotic plays an important role in maintaining water quality parameters and health management as well as increases the survival of shrimp.

  11. Haemocytic defence in black tiger shrimp (Penaeus monodon)

    NARCIS (Netherlands)

    Braak, van de K.

    2002-01-01

    Tropical shrimp culture is one of the fastest growing aquaculture sectors in the world. Since this production sector is highly affected by infectious pathogens, disease control is nowadays a priority. Effective prevention methods can be developed more efficiently when quantitative assays

  12. Immune defence White Spot Syndrome Virus infected shrimp, Penaeus monodon

    NARCIS (Netherlands)

    Arts, J.A.J.

    2006-01-01

    White spot syndrome virus (WSSV) is the most important viral pathogen of cultured penaeid shrimp worldwide. Since the initial discovery of the virus inTaiwanin 1992, it has spread to shrimp farming regions in Southeast Asia, the

  13. White Spot Syndrome Virus infection in Penaeus monodon is ...

    Indian Academy of Sciences (India)

    White Spot Syndrome Virus (WSSV) is a major pathogen in shrimp aquaculture, and its rampant spread has resulted in great economic loss. Identification of host cellular proteins interacting with WSSV will help in unravelling the repertoire of host proteins involved in WSSV infection. In this study, we have employed ...

  14. White Spot Syndrome Virus infection in Penaeus monodon is ...

    Indian Academy of Sciences (India)

    2013-11-06

    assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) to identify the host ..... and early feature of cell death in different cell types triggered to die with different suicidal stimuli (Ucker et al. 2012).

  15. Aplikasi Virtual Reality Media Pembelajaran Sistem Tata Surya

    Directory of Open Access Journals (Sweden)

    I Putu Astya Prayudha

    2017-08-01

    Full Text Available Learning of the solar system is taught since primary school. The learning materials of the solar system are generally explained through blackboards and using books, causing a less of visualization of the solar system that becomes an obstacle in the learning process of the solar system. Develop Virtual Reality Application Learning Media of Solar System in this study aims to facilitate learning with the addition of visualization of the delivery of materials related to the solar system. The application is designed to combine entertainment and knowledge where users interact with virtual environments and see the existence of planets and planets information in the solar system with virtually mode. Application are developed with the delivery of text and voice learning to provide knowledge to users such as the distance the planet to the Sun, diameter, layers, and the constituents of the planet. Users agree Virtual Reality Application Learning Media of Solar System effectively facilitate learning related to the solar system system as evidenced by the questionnaire of 60% for agree value on the content aspect.

  16. ANALISIS TATA KELOLA SISTEM KEUANGAN DI INDONESIA: SEBUAH PERSPEKTIF HISTORIS

    Directory of Open Access Journals (Sweden)

    Lokot Zein Nasution

    2015-07-01

    Full Text Available This study aims to analyze the governance of the financial system in Indonesia. This study uses descriptive analysis by reviewing the literature and analysis of secondary data obtained from the Ministry of Finance, Bank Indonesia (BI, the Central Statistics Agency (BPS. The results of the analysis of this study showed that based on historical experience, to mini-mize internal and external obstacles have to do with governance through improved investment and productive credit. This governance is done by way of coordination between the actors of the financial system, the community, financiers, and government. This model is expected to be a proliferation of governance oriented to minimize the risk of the financial system in the future.

  17. HIPOTESIS SAPIR-WHORF DAN TATA PERGAULAN GENERASI MUDA

    Directory of Open Access Journals (Sweden)

    Diana Hardiyanti

    2011-03-01

    Full Text Available This paper deals with language, culture and thought of the young generation in the society. How they interact among others. Is the eastern culture of Indonesian’s heritage still remaining in the youth?  Interrelationship between language, culture, and thought becomes the basic idea of the Sapir and Whorf hypothesis. The paper will analyze the song lyrics that famous among the youth. How this hypothesis works in the analysis of song lyrics. And can this hypothesis be used to determine the correlation between language, culture and thought of the youth that were written in the lyrics. Keywords: Saphir-Whorf hypothesis, song lyrics, the youth, language, culture and thought.

  18. Kelangsungan dan Perlawanan Tata Pemerintahan yang Baik di Tingkat Desa

    Directory of Open Access Journals (Sweden)

    Christianti Oktiarini

    2010-08-01

    Full Text Available This paper is based on the study of political decentralization and the resistance is carried out in three areas, West Sumatra, Javanese and Batak. Obtained from the three research areas each of these karkteristik peralawanan and sustainability of the local nature of the resistance. There was found to give each region a religious response in the context of regional autonomy that has been ongoing since 1999 in Indonesia. Society against its own way that occasionally showed intensity that it seems it never ceases to deal with the conditions faced daily. While the country is also facing the community in its own way so it does not collide with each other mutual support.

  19. Homi Bhabha Centre for Science Education Tata Institute of ...

    Indian Academy of Sciences (India)

    2018-03-30

    Critical and analytical skills • Commitment to improve education. Science and ... Application submission deadline: March 30, 2018 • Written test: May 13, 2018 • Interview: June. 3rd week ... Those who qualify will be called for an interview.

  20. Pelaksanaan Rencana Tata Bangunan Dan Lingkungan Kawasan Kota Lama Semarang

    OpenAIRE

    Jati, Ardian; Widayati, Wiwik; Astuti, Puji

    2015-01-01

    City of Semarang, has a valuable asset in the form of the Old City, which is a visual image of the splendor of European architecture in the past. The existence of the building and the old building by the Dutch in the Old Town area to be conserved and managed by the Government as stipulated in Law No.11 of 2010 on Heritage and Building Management Plan and Environmental ( RTBL ). The method used in this study is a qualitative research, research that is empirical ( observable by the senses corre...

  1. Tata Kelola Mahasiswa Terhadap Integritas Akademik Dan Plagiarisme

    Directory of Open Access Journals (Sweden)

    Abid Rohmanu

    2016-10-01

    Full Text Available Abstract: This research would like to see how the instituion of Postgraduate Program (PPs STAIN Ponorogo to manage about the scholars academic integrity and plagiarism, and how ideal the respond of PPs STAIN Ponorogo about that scholars’ attitude is. This research is quite important because of many things. First, the scientific writing in short paper (makalah is the entry point of education and teaching in PT. Second, in other side, PPs STAIN Ponorogo is immediately charged to address and respond an indication of plagiarism di the scholars level, and it needs the research as its basis. This research basically is survey quantitatively toward active scholars in Sharia Economic Study amount of 37 scholars. Instrument used is questionnaire with analysis technique of descriptive statistic. This research concludes that as many as 53% of respondents accustomed to cite exactly what words, phrase, sentence say from reference even though not in the context of direct citation (word for word plagiarism. According to it, so the implication of policy can be taken is socialization about academic integrity in any kind and forum, integrating strict rule about plagiarism in the academic guide, shaping an institution “Language Service (Layanan Bahasa”, and “ Assembly of Academic Consideration (Majelis Pertimbangan Akademik” which concern about plagiarism and software of plagiarism detector, and no less important is building academic culture which is full of integrity holistically. Abstrak: Penelitian ini ingin melihat bagaimana pengelolaan mahasiswa PPs STAIN Ponorogo terhadap integritas akademik dan plagiarisme, dan bagaimana idealnya respon kebijakan PPs STAIN Ponorogo terhadap sikap mahasiswa tersebut. Penelitian ini dirasa cukup penting karena beberapa hal. Pertama, penulisan karya ilmiah dalam bentuk makalah merupakan ujung tombak pendidikan dan pengajaran PT. Kedua, di sisi yang lain, PPs STAIN Ponorogo dituntut untuk segera menyikapi dan merespon gejala plagiarisme di tingkat mahasiswa, dan ini membutuhkan penelitian sebagai basisnya. Penelitian ini pada dasarnya adalah penelitian survey yang bersifat kuantitatif terhadap mahasiswa aktif Program Studi Ekonomi Syariah yang berjumlah 37 mahasiswa. Instrumen yang dipakai adalah angket dengan teknik analisis statistik deskriptif. Penelitian ini menyimpulkan bahwa sebanyak 53% dari responden terbiasa untuk mengutip secara persis kata, frase dan kalimat dari sumber rujukan walaupun bukan dalam konteks kutipan langsung (word for word plagiarism. Berdasar hal tersebut, maka implikasi kebijakan yang bisa diambil adalah sosialisasi tentang integritas akademik dalam berbagai bentuk dan forum, mengintegrasikan aturan tegas tantang plagiarisme dalam pedoman akademik, membentuk lembaga “Layanan Bahasa”, dan “Majelis Pertimbangan Akademik” yang konsen pada persoalan plagiarisme dan penyediaan software pendeteksi plagiarisme, dan yang tidak kalah penting adalah membangun kultur akademik yang penuh integritas secara holistik.

  2. HARMONISASI JALINAN DAKWAH DAN POLITIK HUKUM TATA NEGARA

    Directory of Open Access Journals (Sweden)

    Paisol Burlian

    2015-07-01

    Relationship between the two, it was shown that the political ideal function becomes a tool to carry out the mission of preaching. On the other hand Dakwah must also be able to provide an understanding of the political importance of the State constitutional law for the advancement of religion and Muslims, and not vice versa ie away from politics because of the perception that one or manipulate dakwah for political purposes of constitutional law. To ensure that the political activities of the State constitutional law is not lost, it should be understood that in the politics of the State constitutional law is no law that must be followed, while the law is no political aspects of constitutional law which should be implemented. It will allow the synergy of political dakwah and constitutional law for the realization of the glory of Islam in national and international rankings.

  3. Problem Diplomasi Perbatasan dalam Tata Kelola Perbatasan Indonesia-Malaysia

    OpenAIRE

    Fauzan, -; Rachmawati, Iva

    2012-01-01

    The border diplomacy which is intended as tool for gaining national interest should be built on 3 foundations. Those fondations are law, social and economic development and also institution building. This article will highlight the importance of institution building in border diplomacy. In order to manage the cross border relation with neighbour country, the government needs certain institution to holds the task. Related to our case, we will discuss about KK Sosek Malindo as the main institut...

  4. PERANCANGAN ULANG TATA LETAK FASILITAS DENGAN PENDEKATAN LEAN MANUFACTURING

    Directory of Open Access Journals (Sweden)

    Alexander Prasetya

    2016-01-01

    Full Text Available One of the big investment in a business is facility design. It is a long-term investment due to great value. In its development, PT. Dwi Putra Sakti faced some problems related to facility layout. Problems that can be identified, such as work in process that has accumulated on the production floor, as well as the anorganizad facility layout. Therefore, it is necessary to redesign the layout for the production process more effective and efficient. This study uses a lean manufacturing approach to redesign facility layout. It used value stream mapping, seven waste, cellular manufacturing and 5S principle. Analysis of the implementation result is used to design the layout of the new facility. Level layout that will be examined are the macro-and micro-layout layout. Results of macro-layout design is decreasing production cycle time of trousers. While the micro-layout design is decreasing in material handling displacement.

  5. Problem Diplomasi Perbatasan dalam Tata Kelola Perbatasan Indonesia-Malaysia

    Directory of Open Access Journals (Sweden)

    Iva Rachmawati

    2012-11-01

    Full Text Available The border diplomacy which is intended as tool for gaining national interest should be built on 3 foundations. Those fondations are law, social and economic development and also institution building. This article will highlight the importance of institution building in border diplomacy. In order to manage the cross border relation with neighbour country, the government needs certain institution to holds the task. Related to our case, we will discuss about KK Sosek Malindo as the main institution which has been managing the border issues between Indonesia and Malaysia. KK Sosek Malindo routinely meets to solve several problems arise between both countries in the border. Since 1985, they’ve already agreed various cooperations related to border management and border area development. Unfortunately, this institution became useless when cannot implement those agreements and contribute more because distracted by inapropriate structure of KK Sosek Malindo. This article also tend to convince that restructuritation of KK Sosek Malindo should be done in order to optimalize the function of KK Sosek Malindo for better bilateral relation and border area development.

  6. Penerapan Algoritma Genetika Dalam Penentuan Tata Letak Ruang

    OpenAIRE

    Yanda, Rizky

    2014-01-01

    During the making of the basic plan of the home often have difficulty, especially for estimating the layout of each room. Genetic Algorithm can produce a layout that is considered based on space needs. With this system, the ground-plan of making a home can be more convenient and practical. In general, the system can provide a layout results in a land space as expected 081402024

  7. Tata Laksana SAR Minor untuk Mengurangi Rekurensi dan Keparahan

    Directory of Open Access Journals (Sweden)

    Endah Ayu Tri Wulandari

    2013-06-01

    Full Text Available Recurrent aphthous stomatitis (RAS is an inflammatory condition of oral mucosa and is very common that occur in 20% of population, in which 80% of all cases are minor type. Etiology remains unknown but there are factors that can precipitate the attack, known as predisposing factors, which are haematinic deficiency, food hypersensitivity, bacterial or viral infection, hormonal changes, psychological stress, drugs and local trauma. Management of minor RAS are symptomatic and also discovering all predisposing factors which may play role in order to give the appropriate treatments to all predisposing factors. The appropriate treatment for all predisposing factors is to prevent recurrency or reduce the severity of RAS.This paper presents 2 cases of minor RAS which have bacterial infection from poor oral hygiene; local trauma from radices, irritating tooth cusp and rough surface of calculus; haematinic deficiency; physical / psychological stress and food hypersensitivity, as predisposing factors. The management of those cases are scaling, grinding irritating cusp, giving advice of proper and healthy diet including vitamin supplementation, finding any stress factor and also overcome it. We concluded that finding and eliminating predisposing factors can reduce recurrency and severity of RAS.DOI: 10.14693/jdi.v15i2.72

  8. RELASI ISLAM, NEGARA, DAN PANCASILA DALAM PERSPEKTIF TATA HUKUM INDONESIA

    Directory of Open Access Journals (Sweden)

    Hamdan Zoelva

    2012-12-01

    Full Text Available There aredifferences of opinion in looking at the relationship between religion and state. Some proposed that the state should be based on religion (Islam and others have argued the opposite, the state should be separated from the religion (secular nationalism. The debate is considered complete after the Jakarta Charter formula was agreed, although it was annulled after the legalization of the Constitution on August 18, 1945. History shows that Muslims sacrifice for this country is invaluable both in terms of physical struggle (body and soul and ideological (Islamic values. So that, returning authority to the Muslim community to determine the direction of development of the country has become a necessity. Through this article, the author would like to express a new form of moral calling on Muslims to contribute to this country, some of them with sincerity impose Islamic lawinto national law. Terdapat perbedaan pendapat dalam memandang hubungan agama dan negara. Ada yang berpendapat negara  harus  berdasarkan  pada  agama  (Islam  dan  ada  yang  berpendapat sebaliknya,  bahwa negara  harus  dipisah  dari  agama  (nasionalisme  sekuler.  Perdebatan dianggap selesai setelah disepakati rumusan Piagam Jakarta, meskipun kemudian dianulir setelah  disahkannya  UUD  tanggal  18  Agustus  1945.  Sejarah  ini  menunjukkan  bahwa pengorbanan umat Islam untuk negeri ini tidak ternilai harganya baik ditinjau dari segi perjuangan fisik (jiwa raga maupun ideologis (nilai-nilai keislaman. Dari pengorbanan tersebut,  mengembalikan  kewenangan  kepada  umat Islam  untuk  menentukan  arah pembangunan negara ini menjadi keniscayaan. Karena itu melalui tema ini, penulis ingin mengungkapkan bentuk baru panggilan moral umat Islam untuk memberikan konstribusi terhadap negeri ini, yaitu diantaranya melalui keikhlasan memberlakukan hukum Islam ke dalam hukum nasional.

  9. TRF2 and the evolution of the bilateria.

    Science.gov (United States)

    Duttke, Sascha H C; Doolittle, Russell F; Wang, Yuan-Liang; Kadonaga, James T

    2014-10-01

    The development of a complex body plan requires a diversity of regulatory networks. Here we consider the concept of TATA-box-binding protein (TBP) family proteins as "system factors" that each supports a distinct set of transcriptional programs. For instance, TBP activates TATA-box-dependent core promoters, whereas TBP-related factor 2 (TRF2) activates TATA-less core promoters that are dependent on a TCT or downstream core promoter element (DPE) motif. These findings led us to investigate the evolution of TRF2. TBP occurs in Archaea and eukaryotes, but TRF2 evolved prior to the emergence of the bilateria and subsequent to the evolutionary split between bilaterians and nonbilaterian animals. Unlike TBP, TRF2 does not bind to the TATA box and could thus function as a new system factor that is largely independent of TBP. We postulate that this TRF2-based system served as the foundation for new transcriptional programs, such as those involved in triploblasty and body plan development, that facilitated the evolution of bilateria. © 2014 Duttke et al.; Published by Cold Spring Harbor Laboratory Press.

  10. Mechanism of selective recruitment of RNA polymerases II and III to snRNA gene promoters.

    Science.gov (United States)

    Dergai, Oleksandr; Cousin, Pascal; Gouge, Jerome; Satia, Karishma; Praz, Viviane; Kuhlman, Tracy; Lhôte, Philippe; Vannini, Alessandro; Hernandez, Nouria

    2018-05-01

    RNA polymerase II (Pol II) small nuclear RNA (snRNA) promoters and type 3 Pol III promoters have highly similar structures; both contain an interchangeable enhancer and "proximal sequence element" (PSE), which recruits the SNAP complex (SNAPc). The main distinguishing feature is the presence, in the type 3 promoters only, of a TATA box, which determines Pol III specificity. To understand the mechanism by which the absence or presence of a TATA box results in specific Pol recruitment, we examined how SNAPc and general transcription factors required for Pol II or Pol III transcription of SNAPc-dependent genes (i.e., TATA-box-binding protein [TBP], TFIIB, and TFIIA for Pol II transcription and TBP and BRF2 for Pol III transcription) assemble to ensure specific Pol recruitment. TFIIB and BRF2 could each, in a mutually exclusive fashion, be recruited to SNAPc. In contrast, TBP-TFIIB and TBP-BRF2 complexes were not recruited unless a TATA box was present, which allowed selective and efficient recruitment of the TBP-BRF2 complex. Thus, TBP both prevented BRF2 recruitment to Pol II promoters and enhanced BRF2 recruitment to Pol III promoters. On Pol II promoters, TBP recruitment was separate from TFIIB recruitment and enhanced by TFIIA. Our results provide a model for specific Pol recruitment at SNAPc-dependent promoters. © 2018 Dergai et al.; Published by Cold Spring Harbor Laboratory Press.

  11. Cancer Drug-Resistance and a Look at Specific Proteins: Rho GDP-Dissociation Inhibitor 2, Y-Box Binding Protein 1, and HSP70/90 Organizing Protein in Proteomics Clinical Application

    Czech Academy of Sciences Publication Activity Database

    Skalníková, Helena; Martinková, Jiřina; Hrabáková, Rita; Halada, Petr; Dziechciarková, M.; Hajdúch, M.; Gadher, S. J.; Hammar, A.; Enetoft, D.; Ekefjard, A.; Forsstrom-Olsson, O.; Kovářová, Hana

    2011-01-01

    Roč. 10, č. 2 (2011), s. 404-415 ISSN 1535-3893 R&D Projects: GA MŠk LC07017; GA ČR GA301/08/1649 Grant - others:Operational Program Research and Development for Innovations(CZ) CZ.1.05/2.1.00/01.0030 Institutional research plan: CEZ:AV0Z50450515; CEZ:AV0Z50200510 Keywords : cancer * anti-cancer drugs * drug resistance Subject RIV: CE - Biochemistry Impact factor: 5.113, year: 2011

  12. Estimating absolute configurational entropies of macromolecules: the minimally coupled subspace approach.

    Directory of Open Access Journals (Sweden)

    Ulf Hensen

    Full Text Available We develop a general minimally coupled subspace approach (MCSA to compute absolute entropies of macromolecules, such as proteins, from computer generated canonical ensembles. Our approach overcomes limitations of current estimates such as the quasi-harmonic approximation which neglects non-linear and higher-order correlations as well as multi-minima characteristics of protein energy landscapes. Here, Full Correlation Analysis, adaptive kernel density estimation, and mutual information expansions are combined and high accuracy is demonstrated for a number of test systems ranging from alkanes to a 14 residue peptide. We further computed the configurational entropy for the full 67-residue cofactor of the TATA box binding protein illustrating that MCSA yields improved results also for large macromolecular systems.

  13. Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections

    Directory of Open Access Journals (Sweden)

    Müller Marcel A

    2005-02-01

    Full Text Available Abstract Ten potential reference genes were compared for their use in experiments investigating cellular mRNA expression of virus infected cells. Human cell lines were infected with Cytomegalovirus, Human Herpesvirus-6, Camelpox virus, SARS coronavirus or Yellow fever virus. The expression levels of these genes and the viral replication were determined by real-time PCR. Genes were ranked by the BestKeeper tool, the GeNorm tool and by criteria we reported previously. Ranking lists of the genes tested were tool dependent. However, over all, β-actin is an unsuitable as reference gene, whereas TATA-Box binding protein and peptidyl-prolyl-isomerase A are stable reference genes for expression studies in virus infected cells.

  14. Identification of a conserved archaeal RNA polymerase subunit contacted by the basal transcription factor TFB.

    Science.gov (United States)

    Magill, C P; Jackson, S P; Bell, S D

    2001-12-14

    Archaea possess two general transcription factors that are required to recruit RNA polymerase (RNAP) to promoters in vitro. These are TBP, the TATA-box-binding protein and TFB, the archaeal homologue of TFIIB. Thus, the archaeal and eucaryal transcription machineries are fundamentally related. In both RNAP II and archaeal transcription systems, direct contacts between TFB/TFIIB and the RNAP have been demonstrated to mediate recruitment of the polymerase to the promoter. However the subunit(s) directly contacted by these factors has not been identified. Using systematic yeast two-hybrid and biochemical analyses we have identified an interaction between the N-terminal domain of TFB and an evolutionarily conserved subunit of the RNA polymerase, RpoK. Intriguingly, homologues of RpoK are found in all three nuclear RNA polymerases (Rpb6) and also in the bacterial RNA polymerase (omega-subunit).

  15. Activation of silenced cytokine gene promoters by the synergistic effect of TBP-TALE and VP64-TALE activators.

    Science.gov (United States)

    Anthony, Kim; More, Abhijit; Zhang, Xiaoliu

    2014-01-01

    Recent work has shown that the combinatorial use of multiple TALE activators can selectively activate certain cellular genes in inaccessible chromatin regions. In this study, we aimed to interrogate the activation potential of TALEs upon transcriptionally silenced immune genes in the context of non-immune cells. We designed a unique strategy, in which a single TALE fused to the TATA-box binding protein (TBP-TALE) is coupled with multiple VP64-TALE activators. We found that our strategy is significantly more potent than multiple TALE activators alone in activating expression of IL-2 and GM-CSF in diverse cell origins in which both genes are otherwise completely silenced. Chromatin analysis revealed that the gene activation was due in part to displacement of a distinctly positioned nucleosome. These studies provide a novel epigenetic mechanism for artificial gene induction and have important implications for targeted cancer immunotherapy, DNA vaccine development, as well as rational design of TALE activators.

  16. Body Temperature Cycles Control Rhythmic Alternative Splicing in Mammals.

    Science.gov (United States)

    Preußner, Marco; Goldammer, Gesine; Neumann, Alexander; Haltenhof, Tom; Rautenstrauch, Pia; Müller-McNicoll, Michaela; Heyd, Florian

    2017-08-03

    The core body temperature of all mammals oscillates with the time of the day. However, direct molecular consequences of small, physiological changes in body temperature remain largely elusive. Here we show that body temperature cycles drive rhythmic SR protein phosphorylation to control an alternative splicing (AS) program. A temperature change of 1°C is sufficient to induce a concerted splicing switch in a large group of functionally related genes, rendering this splicing-based thermometer much more sensitive than previously described temperature-sensing mechanisms. AS of two exons in the 5' UTR of the TATA-box binding protein (Tbp) highlights the general impact of this mechanism, as it results in rhythmic TBP protein levels with implications for global gene expression in vivo. Together our data establish body temperature-driven AS as a core clock-independent oscillator in mammalian peripheral clocks. Copyright © 2017 Elsevier Inc. All rights reserved.

  17. Indole and synthetic derivative activate chaperone expression to reduce polyQ aggregation in SCA17 neuronal cell and slice culture models

    Directory of Open Access Journals (Sweden)

    Kung PJ

    2014-10-01

    Full Text Available Pin-Jui Kung,1,* Yu-Chen Tao,1,* Ho-Chiang Hsu,1 Wan-Ling Chen,1 Te-Hsien Lin,1 Donala Janreddy,2 Ching-Fa Yao,2 Kuo-Hsuan Chang,3 Jung-Yaw Lin,1 Ming-Tsan Su,1 Chung-Hsin Wu,1 Guey-Jen Lee-Chen,1 Hsiu-Mei Hsieh-Li1 1Department of Life Science, 2Department of Chemistry, National Taiwan Normal University, Taipei, Taiwan; 3Department of Neurology, Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Taipei, Taiwan *These authors contributed equally to this work Abstract: In spinocerebellar ataxia type 17 (SCA17, the expansion of a translated CAG repeat in the TATA box binding protein (TBP gene results in a long polyglutamine (polyQ tract in the TBP protein, leading to intracellular accumulation of aggregated TBP and cell death. The molecular chaperones act in preventing protein aggregation to ameliorate downstream harmful events. In this study, we used Tet-On SH-SY5Y cells with inducible SCA17 TBP/Q79-green fluorescent protein (GFP expression to test indole and synthetic derivative NC001-8 for neuroprotection. We found that indole and NC001-8 up-regulated chaperone expression to reduce polyQ aggregation in neuronal differentiated TBP/Q79 cells. The effects on promoting neurite outgrowth and on reduction of aggregation on Purkinje cells were also confirmed with cerebellar primary and slice cultures of SCA17 transgenic mice. Our results demonstrate how indole and derivative NC001-8 reduce polyQ aggregation to support their therapeutic potentials in SCA17 treatment. Keywords: spinocerebellar ataxia type 17, TATA box binding protein, polyQ aggregation, indole and derivative, therapeutics

  18. Deep-diving by narwhals Monodon monoceros: differences in foraging behavior between wintering areas?

    DEFF Research Database (Denmark)

    Laidre, K. L.; Heide-Jørgensen, M. P.; Dietz, R.

    2003-01-01

    Variation in resource selection among sub-populations may elucidate differences in fitness and life history strategies. Specifically for top marine predators, differences in movements and behavior may result from responses to variation in a patchy, dynamic environment. Satellite-linked time...

  19. TRANSFER GEN ANTIVIRUS PADA EMBRIO UDANG WINDU, Penaeus monodon DALAM BERBAGAI KONSENTRASI DEOXYRIBO NUCLEIC ACID

    Directory of Open Access Journals (Sweden)

    Andi Parenrengi

    2011-12-01

    Full Text Available Teknologi transgenesis khususnya rekayasa genetik untuk menghasilkan udang windu resisten penyakit merupakan salah satu strategi yang dapat dilakukan dalam upaya pemecahan masalah penyakit yang menimpa budidaya udang windu. Teknologi transgenesis khususnya transfer gen antivirus pada udang windu telah berhasil dilakukan melalui teknik transfeksi. Meskipun demikian optimalisasi komponen teknologi tersebut masih perlu dilakukan. Konsentrasi DNA gen merupakan salah satu komponen teknologi transgenesis yang harus dioptimalkan untuk mendapatkan efisiensi dalam transfer gen. Penelitian bertujuan untuk mengetahui konsentrasi DNA gen antivirus yang optimal sebagai bahan transfer gen ke embrio menggunakan metode transfeksi. Embrio udang windu yang diperoleh dari hasil pemijahan induk asal Aceh, dikoleksi 5-10 menit setelah memijah dengan kepadatan 625 telur/2 mL. Transfeksi dilakukan dengan menggunakan media larutan transfeksi jetPEI dengan konsentrasi DNA gen antivirus sebagai perlakuan, yakni: 5, 10, dan 15 µg serta kontrol positif (tanpa plasmid DNA dan negatif (tanpa plasmid DNA dan larutan transfeksi, masing-masing 3 ulangan. Embrio hasil transfeksi ditetaskan pada stoples berisi air laut sebanyak 2 L yang diletakkan pada waterbath. Hasil penelitian menunjukkan bahwa gen antivirus telah berhasil diintroduksi ke embrio udang windu. Hasil analisis ragam menunjukkan bahwa perbedaan konsentrasi DNA (5-15 µg tidak berpengaruh nyata (P>0,05 terhadap daya tetas embrio udang windu. Analisis ekspresi gen pada larva udang windu juga menunjukkan adanya aktivitas ekspresi gen antivirus pada semua perlakuan konsentrasi DNA, di mana ekspresi gen antivirus pada larva transgenik lebih tinggi dibandingkan dengan kontrol (tanpa transfeksi. Sintasan pasca-larva PL-1 yang didapatkan pada penelitian ini adalah 12,0%; 10,0%; 10,6%; 12,3%; dan 14,2% masing-masing untuk perlakuan konsentrasi plasmid DNA 5 µg, 10 µg, 15 µg, kontrol positif dan negatif, di mana konsentrasi DNA yang berbeda tidak memperlihatkan pengaruh nyata (P>0,05 terhadap sintasan larva PL-1. Hasil penelitian ini berimplikasi bahwa untuk alasan efisiensi, konsentrasi DNA 5 µg disarankan untuk digunakan dalam transfer gen pada embrio udang windu.

  20. EXPRESSION OF ANTIVIRAL GENE ON TIGER SHRIMP Penaeus monodon AT DIFFERENT TISSUE AND BODY SIZE

    Directory of Open Access Journals (Sweden)

    Andi Parenrengi

    2012-12-01

    Full Text Available The role of tiger shrimp defense against invading pathogen on molecular level such antiviral gene expression is limited to be reported. Gene expression is a process which codes information of genes that is converted to the protein as a phenotype. Distribution of PmAV antivirus gene, that has been reported as an important gene on non-specific response immune, is needed to be observed to several organs/tissues and size of tiger shrimp. The aim of this study is to determine the distribution of gene antiviral expression at several organ/tissue and size of shrimp. The organs/tissues observed in this study were: gill, hepatopancres, muscle tissue, eyes, heart, stomach, gonad, and intestine. While the size of shrimp consisted of three groups, those are: (A 10-20 g/ind., (B 30-40 g/ind., and (C 60-70 g/ind. Analysis of antiviral gene expression was performed by RNA extraction, followed by the cDNA syntesis, and amplification of gene expression by semi-quantitative PCR. The result of PCR optimation showed the optimal concentration of cDNA and primer was 1 μL and 50 mol, respectively for PCR final volume of 25 μL. Antiviral gene was expressed on the hepatopancreas and stomach in percentage of 50.0% and 16.7%, respectively. While the highest percentage of individual expressing the antiviral gene was observed in the shrimp size of C (66.7%, followed by B (50.0% and A (16.7%. The result of study implied that the hepatopancreas has importantly involed in tiger shrimp defense mechanism on viral infection.

  1. Efficiency of probiotics (Ecoforce) in the growth and survival of Peneaus monodon

    OpenAIRE

    V. Elumalai; G. Ramesh; D. Mohan, et al.

    2013-01-01

    Probiotic supplementation of live microorganisms in aquaculture aids in preventing disease, thereby increasing production and decreasing economic loss. Application of probiotics bacteria in aquaculture systems plays significant role that determines the fate and success rate of culture. The present study was carried out to evaluate the performance of commercially available probiotics (The major active ingredients include Streptococcus faecalis, Streptococcus faecium, Bacillus mesentericus, Bac...

  2. Paradoxical escape responses by narwhals (Monodon monoceros)

    DEFF Research Database (Denmark)

    Williams, Terrie M.; Blackwell, Susanna B.; Richter, Beau

    2017-01-01

    -regulation (extreme bradycardia with heart rate ≤4 beats per minute) superimposed on exercise up-regulation (stroke frequency >25 strokes per minute and energetic costs three to six times the resting rate of energy expenditure) that rapidly depleted onboard oxygen stores. We attribute this unusual reaction...

  3. Vibrio harveyi modulated gene expression in Penaeus monodon and Fenneropenaeus indicus

    Digital Repository Service at National Institute of Oceanography (India)

    Nayak, S.

    : Gammaproteobacteria Order : Vibrionales Family : Vibrionaceae Genus : Vibrio Species : harveyi Vibrio harveyi is a Gram-negative bioluminescent marine bacterium. It is both a free- living, as well as a symbiont with many... marine animals. In free-living form, it can be found throughout the water column, in the sediment as well as on the exterior surfaces of marine organisms. It causes systemic infection resulting in mortalities in larvae and post-larvae, which sometimes...

  4. Effect of new aeration technology on the bacteriology of shrimp ponds growing Penaeus monodon

    Digital Repository Service at National Institute of Oceanography (India)

    Karekar, S.V.; Sreepada, R.A.; Shirodkar, R.R.; Kulkarni, S.; Kumar, P.; LokaBharathi, P.A.; Bergheim, A.; Vogelsang, C.

    in the recent past arid are mentioned for their principle, use and performance (Boyd, 1998) in aquaculture practices. HOBAS technology developed by HOBAS Water Engineering. Norway mechanically mixes air and water in order to replace oxygen deficits... and competition from other user community of natural resources. Intensification of farming practices can have an adverse affect on the health of the farm as a result of stressed conditions, which may result in the outbreak of disease (Bachere. 2000; Otta et a...

  5. PENGARUH PEMBERIAN EKSTRAK PAKIS SEBAGAI MOULTING STIMULAN PADA INDUK UDANG WINDU (Penaeus monodon. Fab DI HATCHERY

    Directory of Open Access Journals (Sweden)

    Emma Suryati

    2013-08-01

    kandungan senyawa steroid yaitu fitoekdisteroid dalam bentuk 20-Hydroxyecdyson atau Ecdysteron berfungsi sebagai moulting stimulan pada krustase. Pada umumnya ecdysteron ditemukan pada krustase baik yang ada di darat maupun yang berada di dalam air seperti kepiting, udang, dan krustase lainnya yang ditemukan secara alami dan berfungsi sebagai pengatur proses penggantian kulit dan mengontrol pembentukan exoskeleton baru untuk menggantikan exoskeleton yang lama. Selain ablasi proses moulting pada udang dapat diinduksi melalui penambahan 20-hydroksi ecdysteron (20 E pada hemolim sehingga fase premoulting dapat diatur sesuai dengan kebutuhan. 20-hydroksiecdysteron dapat diperoleh dari ekstrak tumbuhan di antaranya bayam, asparagus, pakis, dan lain-lain melalui pemisahan dengan ekstraksi, fraksinasi, dan pemurnian dengan HPLC dilanjutkan dengan elusidasi struktur. Pemberian ekstrak pakis pada induk udang windu untuk memacu terjadinya pergantian kulit dilakukan melalui dengan beberapa konsentrasi menggunakan desain Rancangan Acak Lengkap (RAL. Hasil analisis memperlihatkan bahwa kandungan phytoecdysteron pada pakis perkisar 230-730 mg/L dari larutan ekstrak yang setara dengan 20 g bahan segar. Konsentrasi ECD 25 mg/L, memperlihatkan respon yang paling baik sebagai moulting stimulan.

  6. Identification of upregulated immune-related genes in Vibrio harveyi challenged Penaeus monodon postlarvae

    Digital Repository Service at National Institute of Oceanography (India)

    Nayak, S.; Singh, S.K.; Ramaiah, N.; Sreepada, R.A.

    at the early life-stages of shrimps are of great significance. An understanding therefore, of molecular responses and defense mechanism(s) in shrimps against bacterial pathogens is essential. Besides production of antimicrobial biomolecules, the innate... of this strain grown for 24h in seawater nutrient broth were used for the experimental challenge. Cells were harvested by centrifugation and re-suspended in sterilized seawater and the PL were ‘bath’ challenged by adding the harvested cells to attain 2.5- 3...

  7. Low-input Modified Extensive Shrimp Culture System for Penaeus monodon Restrian Vibriosis

    Digital Repository Service at National Institute of Oceanography (India)

    Fernandes, S.O.; Sreepada, R.A.; Kulkarni, S.S.; Karekar. S.V.; Shirodkar, R.R.; Vogelsang, C.; LokaBharathi, P.A.

    by the polymerase chain reaction test were procured from a local hatchery. Larvae were actively moving around with no visible signs of disease or morbidity and were transferred into the two production ponds at a stocking density of 5 PL m-2. No water exchange... and Kitao (1991). Additional biochemical tests were performed as per standard methods and compared with standard reactions for confirmation (Alsina and Blanch, 1994; Holt et al., 1994). Species level identification was carried out wherever possible...

  8. Penaeus monodon post-larvae and their interaction with Rhizophora apiculata

    NARCIS (Netherlands)

    Nga, B.T.

    2004-01-01

    In recent years, expansion of shrimp aquaculture in Vietnam has brought considerable financial benefits to farmers and local communities. In the coastal provinces in the Mekong Delta, brackish shrimp aquaculture is the major economy activity. Extensive shrimp-mangrove culture systems are popularly

  9. Penaeus monodon post-larvae and their interaction with Rhizophora apiculata

    OpenAIRE

    Nga, B.T.

    2004-01-01

    In recent years, expansion of shrimp aquaculture in Vietnam has brought considerable financial benefits to farmers and local communities. In the coastal provinces in the Mekong Delta, brackish shrimp aquaculture is the major economy activity. Extensive shrimp-mangrove culture systems are popularly practiced here. Although the average shrimp production is low, due to over-exploitation and destruction of mangrove forests and salt marshes, these systems are of special interest in view of the pro...

  10. POTENCY OF VIBRIO ISOLATES FOR BIOCONTROL OF VIBRIOSIS IN TIGER SHRIMP (PENAEUS MONODON LARVAE

    Directory of Open Access Journals (Sweden)

    B. W. LAY

    2003-01-01

    Full Text Available This study was carried out to obtain Vibrio isolates able to function as biocontr ol of vibriosis in shrimp hatchery. Thirty one Vibrio isolates were isolated from tiger shrimp larvae and hatchery environments, i.e. Labuan, Pangandaran, and Lampung, Indonesia. Pathogenic V. harveyi MR5339 was obtained from Maros, South-Sulawesi and was made as a rifampicin resistant mutant (RFR to screen for those 31 Vibrio isolates in in vitro assays and to allow us to monitor their presence in shrimp larvae and larval rearing water. Almost all Vibrio isolates could inhibit the growth of pathogenic V. harveyi MR5339 RFR. SKT-b isolate from Skeletonema was the most effective to inhibit the growth of V. harveyi MR5339 Rf* and significantly reduced larval mortality in pathogen challenge assays. These prospective biocontrol bacteria, at concentration of 10" CFU/ml, did not show pathogenicity to shrimp larvae. SKT-b was Gram negative, short rod-shape, exhibited yellow colonies on TCBS and swarming on SWC-agar media, motile, u tilized glucose and sucrose but not lactose: produced extra-cellular protease and amylase, but did not produce chitmase. Partial sequencing of 16S-rRNA gene SKT-b showed SKT-b similarity to Vibrio alginofyticus.

  11. PENTOKOLAN UDANG WINDU, Penaeus monodon DENGAN KEPADATAN BERBEDA DALAM KERAMBA JARING APUNG (KJA DI LAUT

    Directory of Open Access Journals (Sweden)

    Syarifuddin Tonnek

    2016-11-01

    Full Text Available Produksi tokolan udang windu PL 54 menggunakan hapa pada unit keramba jaring apung (KJA di laut telah dilakukan di Teluk Labuange, Kabupaten Barru dari bulan Mei—Juli 2002. Penelitian ini bertujuan mendapatkan padat penebaran terbaik untuk pendederan benur udang windu dalam KJA di laut. Perlakuan yang dicobakan adalah padat penebaran benur PL 12 sebanyak padat tebar 2.000 ekor/m2 (A, 3.000 ekor/m2 (B, dan 4.000 ekor/m2 (C masing-masing 3 kali ulangan. Hasil penelitian menunjukkan bahwa pertumbuhan tokolan pada perlakuan 2.000 dan 3.000 ekor/m2 tidak berbeda nyata (0,57—0,65 g/ekor (P>0,05, tetapi berbeda nyata pada padat penebaran 4.000 ekor/m2 (0,39 g/ekor (P0.05, but significantly different with stocking density of 4,000 pcs/m2 (P<0.05. Nevertheless average survival rate of juvenile shrimps was not significant on stocking density 2,000 pcs and 3,000 pcs/m2, but significant different with stocking density of 4,000 pcs/me.Therefore, for juvenill production in floating net cages, the best (maximum stocking density of potensial 12 is 3,000 pcs/m2.

  12. PENYEBARAN DAN PREVALENSI WHITE SPOT SYNDROME VIRUS (WSSV PADA BUDI DAYA UDANG WINDU (Penaeus monodon

    Directory of Open Access Journals (Sweden)

    Muliani Muliani

    2016-11-01

    The aims of this experiment was to know the distribution and prevalences of WSSV on tiger shrimp. Sample collection and WSSV detection conducted with PCR method was carried out during April 2004 to November 2006. Tiger shrimp broodstock samples were collected from Central Java, South Sulawesi, Gorontalo, Kalimantan, and Timika waters, and the other samples (tiger shrimp post larvae, juveniles, and cultured shrimp were collected from several region in South Sulawesi. The pleopod, pereiopod, eye stalk, carapax, gill, tail, muscle of broodstock, juveniles, and cultured shrimp, and 30 pcs of postlarvae were extracted using lysis buffer to collect genomic DNA. WSSV DNA amplification was carried out using first and nested PCR technique by specific sequence amplification kit (IQ2000TM  Detection and Prevention system. The WSSV DNA was visualized by gell documentation system. The result showed that more than 33% of broodstock resources of Indonesia waters and 90% of shrimp culture area of South Sulawesi were contaminated by WSSV. WSSV was also infected tiger shrimp broodstock, postlarvae, juveniles, and tiger shrimp cultured with the highest prevalence (40.4% was on tiger shrimp cultured and the lowest prevalence (5.4% was on postlarvae.

  13. Isolation of probiotics bacterium from coral reef for controlling vibriosis in tiger shrimp (Penaeus monodon larvae

    Directory of Open Access Journals (Sweden)

    Ade Dwi Sasanti

    2013-03-01

    Full Text Available Pathogenic Vibrio, especially luminous Vibrio harveyi, could cause mass mortality in tiger shrimp culture. One of the technique to work against luminous Vibrio is, using probiotic bacteria to inhibit the luminous Vibrio growth. This study was carried out to obtain bacteria isolates from coral reef which potentially inhibit V. harveyi growth. A total of 110 isolates were isolated from Acropora sp, Merulina sp, Hystrix sp., Poecillophora sp, Porites sp and Haliophora sp., and have probiotic activity against V. harveyi in in vitro and in vivo test.Of the total 110 isolates, 54 isolates show the inhibiting zone. Two isolates(8A and 1Cwere not pathogenic and have the most effective activity in inhibiting growth of V. harveyi and significantly reduced larval mortality in in vitro and in vivo test. Treatment using probiotics candidate have significant different survival rate (83.33% compared with positive control (61.67%. The growth rate of lenght of larvae treatment with isolate of 8A (5.25% and 1C (5.06% show the significant different compared with positive control (3.54%. The growth rate of weight of larvae treatment withisolate of 8A (17.51% and 1C (17.61% show significant different compared with negative (15.27% and positive control (14.69%.Key word: coral reef, probiotic, tiger shrimp, vibriosis, V. harveyi. ABSTRAKVibrio patogen, khususnya Vibrio harveyi berpendar, dapat menyebabkan kematian massal pada budidaya udang windu. Salah satu alternatif untuk menghambat Vibrio harveyi berpendar adalah dengan menggunakan bakteri probiotik yang dapat menekan pertumbuhan Vibrio tersebut. Penelitian ini bertujuan untuk mendapatkan isolat bakteri dari terumbu karang yang potensial menghambat pertumbuhan V. harveyi. Total 110 isolat diisolasi dari Acropora sp, Merulina sp., Hystrix sp., Poecillophora sp., Porites sp. dan Haliophora sp, dilakukan penapisan untuk melihat aktivitas kemampuannya melawan V. harveyi MR 5339 RfR dalam uji in vitro dan uji in vivo. Sebanyak 54 isolat teridentifikasi mampu menghasilkan zona hambat. Dua isolat (8A dan 1C terbukti tidak bersifat patogen dan memiliki aktivitas probiotik melawan V. harveyi MR 5339 RfR pada uji in vitro dan in vivo. Tingkat kelangsungan hidup larva pada perlakuan yang diberi kandidat probiotik dari kedua isolat tersebut adalah 83,33%, dan menunjukkan hasil yang berbeda nyata dengan kontrol positif (61,67%. Laju pertumbuhan panjang larva yang diberi isolat 8A (5,25% dan 1C (5,06%menunjukkan hasil yang berbeda nyatajika dibandingkan dengan kontrolpositif (3,54%. Laju pertumbuhan bobot yang diberi isolat 8A (17,51% dan 1C (17,61% menunjukkan hasil yang berbeda nyata terhadap kontrol negative (15,27% dan kontrol positif (14,69%.Kata kunci: probiotik, terumbu karang,udang windu, V. harveyi, vibriosis.

  14. Detection of Vibrio harveyi using hemolysin primer in tiger shrimp Penaeus monodon

    Directory of Open Access Journals (Sweden)

    Irma Suriyani

    2015-05-01

    Full Text Available ABSTRACT This study was aimed to analyze the sensitivity and ability of primer hemolysin in detecting pathogenetic Vibrio on tiger shrimp post-larvae (PL exposed under different exposure times in media inoculated with Vibrio harveyi. The PL of tiger shrimp were infected with 106 cfu/mL of V. harveyi by immersion method for three, six, 12, 24, 48 and 72 hours. The presence of hemolisin genes was detected by PCR techniques. The electrophoresis detected narrow hemolysin genes after PL were exposed for three and six hours. Clear visible bands of DNA Vibrio were observed for 12 hours of exposure. In contrast, no detected hemolysin gene of Vibrio was observed for PL exposed within 24, 48, and 72 hours. The rapid detection on Vibrio pathogenic for tiger shrimp PL should be conducted within three to 12 hours of exposure. No recommendation in utilizing this rapid detection for tiger shrimp PL exposed beyond 12 hours of V. harveyi. Keywords: specific primer, luminous Vibrio bacteria, pathogenic, PCR method, hemolysin gene  ABSTRAK Penelitian ini bertujuan untuk mengetahui kemampuan atau sensitivitas primer hemolisin dalam mendeteksi Vibrio patogen dengan lama pemaparan berbeda. Penelitian ini dilakukan dengan menginfeksikan Vibrio harveyi pada benur udang dengan metode perendaman pada konsentrasi 106 cfu/mL. Pengambilan sampel dilakukan pada waktu tiga, enam, 12, 24, 48, dan 72 jam pascainfeksi. Keberadaan gen hemolisin pada bakteri V. harveyi dideteksi menggunakan teknik polymerase chain reaction (PCR. Hasil elektroforesis memperlihatkan bahwa pada pemaparan tiga dan enam jam keberadaan gen hemolisin dari bakteri Vibrio patogen yang diinfeksikan sudah dapat terdeteksi pada benur walaupun masih terlihat tipis. Pada pemaparan 12 jam terlihat sangat jelas pita-pita DNA dari bakteri patogen. Sedangkan pada pemaparan 24, 48, dan 72 jam sudah tidak terdeteksi lagi gen hemolisin dari bakteri Vibrio. Hal ini diduga disebabkan terjadinya penurunan populasi bakteri Vibrio yang hidup dalam tubuh dan media pemeliharaan udang. Pentingnya deteksi cepat diawal udang terinfeksi bakteri (3–12 jam karena setelah 12 jam infeksi sudah sulit untuk mendeteksi keberadaan bakteri patogen di dalam tubuh udang. Kata kunci: primer spesifik, bakteri Vibrio berpendar, patogenik, metode PCR, gen hemolisin 

  15. QUALITY IMPROVEMENT OF DISQUALIFIED TIGER PRAWN (PENAEUS MONODON FAB. BROODSTOCK USING DOPAMINE

    Directory of Open Access Journals (Sweden)

    ETTY RIANI

    2010-01-01

    Full Text Available Disqualified tiger prawn broodstock has undergone ablation after two breeding periods, which causes high production cost, pollution of environment and difficulties in providing the broodstock continuously with enough quantity at an exact time. The purpose of this study was to find the proper dosage of dopamine to improve the quality for breeding of the disqualified tiger prawn, because dopamine inhibits maturation, so there will be enough time for maturation and improvement of the eggs quality.This experiment used 15 pairs of the twice breeding tiger prawn, which was collected from -9Perigi (Central Java. The samples were treated with different dosages of dopamine (dosage 10 , -8 -7 -6 10 , 10 , 10 moll and control/injected with aquabidest. The parameters identified in this experiment were duration (days for eggs maturation, fecundity, fertilizing rate, hatching rate, egg diameter and phototaxis response of the nauplii.The research concluded that the average duration for eggs maturation on the broodstock -9 -8 -7 -6that were injected with dopamine, dosage 10 , 10 , 10 , 10 moll and control, were 14, 12, 4, 2, 5 days, respectively. Fecundities were 561.489, 503.000, 384.205, 230.850 and 150.034. Fertilizing rates were 89.8%, 82.1%, 56.0%, 46.3%, and 23.2%. Hatching rates were 79.53%, 72.53%, 47.75%, 12.27%, and 36.6%. Diameters were 0.29; 0.29; 0.29; 0.27; and 0.27 mm. The nauplii -9 -8 -7produced by injecting broodstock with dopamine dosages of 10 , 10 , 10 , have positive -6phototaxis response, while for dosage 10 and control the responses were weak.

  16. Advantages of liquid nitrogen freezing of Penaeus monodon over conventional plate freezing

    OpenAIRE

    Chakrabarti, R.; Chaudhury, D.R.

    1987-01-01

    Liquid nitrogen frozen products are biochemically and organoleptically superior to conventional plate frozen products but beneficial effect of liquid nitrogen freezing over conventional plate freezing can exist only up to 59 days at a commercial storage temperature of -18°C.

  17. Studies on luminous, Vibrio harveyi associated with shrimp culture system rearing Panaeus monodon

    Digital Repository Service at National Institute of Oceanography (India)

    Kannapiran, E.; Ravindran, J.; Chandrasekar, R.; Kalaiarasi, A.

    feed, fertilizers and vitamins. This observation reconciles with the earlier findings that the artificial feed and other organic additions in the aquaculture farms enhanced the heterotrophic bacterial population (MacLean et al., 1994; Sharmila et al... the pond bottom preparation and low level of organic matter in the pond. As culture period progresses, accumulation of organic wastes in the shrimp pond takes placed due to the application of organic manure and fertilizers, feed waste, fecal matter...

  18. SELEKSI BAKTERI PROBIOTIK UNTUK BIOKONTROL VIBRIOSIS PADA LARVA UDANG WINDU, Penaeus monodon MENGGUNAKAN CARA KULTUR BERSAMA

    Directory of Open Access Journals (Sweden)

    Widanarni Widanarni

    2009-04-01

    Full Text Available Penelitian ini bertujuan untuk memperoleh bakteri probiotik yang mampu menghambat pertumbuhan Vibrio harveyi mengggunakan metode kultur bersama. Sebanyak 51 isolat kandidat probiotik berhasil diisolasi dari larva udang dan lingkungan pemeliharaannya di Balai Pengembangan Benih Ikan Air Payau dan Udang (BPBILAPU, Pangandaran serta hatcheri udang PT Biru Laut Khatulistiwa dan tambak udang intensif di Lampung. Dari total isolat tersebut setelah diseleksi secara in vitro menggunakan metode kultur bersama dipilih 3 isolat kandidat probiotik yang paling potensial dalam menekan atau menghambat pertumbuhan V. harveyi MR 5399 RfR yakni 1Ub, P20Bf, dan 10a. Ketiga isolat tersebut selanjutnya digunakan pada uji patogenisitas dan uji tantang pada larva udang windu. Hasil uji patogenisitas dengan konsentrasi bakteri 106 CFU/mL menunjukkan bahwa ketiga isolat tersebut tidak bersifat patogen pada larva udang windu. Hasil uji tantang pada larva udang juga menunjukkan bahwa ketiga isolat tersebut mampu meningkatkan sintasan larva udang windu. Nilai sintasan larva pada perlakuan yang selain diinfeksi dengan V. harveyi MR5399 RfR juga ditambah probiotik 1Ub, P20Bf, dan 10a masing-masing adalah 90,0%; 86,7%; dan 78,3% sedangkan pada perlakuan yang hanya diinfeksi dengan V. harveyi MR5399 RfR tanpa probiotik nilai sintasannya hanya mencapai 73,3%. Populasi bakteri V. harveyi pada perlakuan dengan penambahan bakteri probiotik lebih rendah dibanding perlakuan tanpa probiotik, hal ini menunjukkan kemungkinan adanya kompetisi antara bakteri V. harveyi dengan 1Ub. This research was aimed to obtain probiotic bacteria that can be used to inhibit the growth of Vibrio harveyi using co-culture method. This method succeeded in isolating 51 probiotic bacteria candidates from shrimp larva and their rearing environment in Balai Pengembangan Benih Ikan Laut Payau dan Udang (BPBILAPU, Pangandaran and shrimp hatchery of PT Biru Laut Khatulistiwa and intensively managed shrimp pond in Lampung. After in vitro selection of the total isolates using co-culture method, three most potential probiotic bacteria candidates in inhibiting or suppressing growth of V. harveyi MR 5399 RfR bacteria were chosen. The three isolates were then used in pathogenicity and challenge test in tiger shrimp larva. Results of pathogenicity test at the concentration of 106 CFU/mL bacteria showed that the three isolates were not pathogen to tiger shrimp larvae. Challenge test results in shrimp larvae also showed that the three isolates could increase survival rates of tiger shrimp larva. Larva survival rate value of treatment using V. harveyi MR5399 RfR with 1Ub, P20Bf, dan 10a probiotic were 90.0%, 86.7% dan 78.3%, respectively; whereas infection treatment merely using V. harveyi MR5399 RfR without probiotic only gave 73.3% survival rate. V. harveyi population in treatment with addition of probiotic bacteria were lower than that of without probiotic. This suggested the existence of possible competition between V. harveyi and 1Ub bacteria.

  19. Studies on luminous, Vibrio harveyi associated with shrimp culture system rearing Penaeus monodon.

    Science.gov (United States)

    Kannapiran, E; Ravindran, J; Chandrasekar, R; Kalaiarasi, A

    2009-09-01

    Microbiological studies in a modified extensive shrimp culture system at Nambuthalai, southeast coast of India were carried out fora period of 120 days. Population dynamics and distribution profile of luminous bacteria and total heterotrophic bacteria in the water sediment and animal samples were monitored. Luminous bacteria associated with exoskeleton, gills and gut were isolated and quantified. The total heterotrophic bacterial counts ranged from 1.3 x 10(4) to 25.3 x 10(4) CFU ml(-1) in water and 1.5 x 10(6) to 26.2 x 10(6) CFU g(-1) in sediment. The V. harveyi population density varied between 0.6 x 10(4) and 8.8 x 10(4) LCFU ml(-1) in water and from 1.2 x 10(6) to 10.4 x 10(6) LCFU g(-1) sediment respectively. The gut of the animal was found to harbor high density of V. harveyi than gills and exoskeleton. The total heterotrophic bacteria and V. harveyi population density showed increasing trend during the culture period. The high V harveyi density observed in this study at the end of the culture period correlated with the outbreak of white spot disease.

  20. Protection of Penaeus monodon against White Spot Syndrome Virus by oral vaccination

    NARCIS (Netherlands)

    Witteveldt, J.; Cifuentes, C.; Vlak, J.M.; Hulten, van M.C.W.

    2004-01-01

    White spot syndrome virus (WSSV) occurs worldwide and causes high mortality and considerable economic damage to the shrimp farming industry. No adequate treatments against this virus are available. It is generally accepted that invertebrates such as shrimp do not have an adaptive immune response

  1. APLIKASI INSEMINASI BUATAN PADA UDANG WINDU, Penaeus monodon ALAM MENGGUNAKAN SUMBER DAN JUMLAH SPERMATOFOR YANG BERBEDA

    Directory of Open Access Journals (Sweden)

    Samuel Lante

    2017-01-01

    Full Text Available Salah satu kendala utama dalam domestikasi udang windu adalah rendahnya tingkat perkawinan secara alami dalam wadah budidaya. Hal yang sama terjadi pada udang windu alam yang digunakan di unit pembenihan. Salah satu upaya untuk mendapatkan telur fertil adalah melalui inseminasi buatan (IB. Inseminasi buatan merupakan teknik mentransfer spermatofor dari induk jantan dengan cara memasukkannya ke dalam telikum udang betina. Penelitian ini bertujuan untuk mengetahui performa reproduksi udang windu betina alam pasca-inseminasi menggunakan sumber dan jumlah spermatofor induk jantan alam yang berbeda. Penelitian dilakukan dua tahap yaitu 1 IB menggunakan spermatofor induk jantan dari perairan Sulawesi Selatan (SS dan spermatofor induk jantan dari Aceh (SA dan 2 IB menggunakan jumlah spermatofor berbeda yaitu satu spermatofor (S-1 dan dua spermatofor (S-2 pada udang windu betina alam. Inseminasi spermatofor dilakukan pada induk udang windu betina setelah dua hari moulting. Hasil yang diperoleh pada IB tahap pertama menunjukkan bahwa daya tetas telur udang windu betina alam lokal tidak dipengaruhi oleh sumber (lokasi asal udang jantan, di mana daya tetas telur relatif sama pada kedua perlakuan, yaitu 61,6% pada SS dan 61,7% pada SA. IB pada tahap kedua menunjukkan bahwa daya tetas telur fertil yang diperoleh pada S-2 sebesar 40,5%; lebih rendah dari S-1 sebesar 44%. One of the main constraints in the domestication of black tiger shrimp is very low natural mating in the tank. Similar condition have been happened in commercial hatcheries. An effort to improve the eggs fertility is through artificial insemination (AI. This study aimed to know reproductive performance of wild black tiger shrimp after insemination with different sources and numbers of spermatophore. This study consisted of two trials.The first one was AI using spermatophores of wild male obtained from two different locations, namely from South Sulawesi (SS and Aceh (SA. The second trial was AI using different numbers of spermatophore namely one spermatophore (S-1 and two spermatophores (S-2. AI was applied to the females at two days post-moulting. The results of the first trial showed that the hatching rate (HR was not affected by the source of the male which was 61.6% for SS and 61.7% for SA. The second trial indicated that female inseminated S-2 had lower HR than S-1 (40.5% vs 44%.

  2. TATA CARA PELAKSANAAN PENANGGUHAN PENAHANAN TERHADAP TERSANGKA DI LEMBAGA KEPOLISIAN BERDASARKAN KUHAP DAN PERATURAN PELAKSANAANNYA

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    Umi Enggarsasi

    2005-10-01

    Full Text Available Detention pursuant to KUHAP is location or certain defendant in place by Investigator or public prosecutor or Judge with its stipulating, while reason to source the detention as arranged in Section 21 KUHAP mentioned that detention can be with the consideration for example, defendant felt concerned about to break away defendant felt concerned about will destroy eliminating evidence goods, and, defendant felt concerned about will again doing an injustice. Defendant can ask the detention deferment based on Section 31 KUHAP to police detention with the guarantee of money or people or on trust pursuant to condition determined, while such condition determined that is obliged to report, do not go out the house or town, and a period to detention deferment from defendant do not the inclusive of a period to prisoner status as arranged in KUHAP.

  3. Tata Laksana Kasus Perempuan dengan Pure Gonadal Dysgenesis 46, XY (Sindrom Swyer

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    Kanadi Sumapradja

    2016-06-01

    Full Text Available Disorders of sex development (DSD adalah kelainan medis yang dikaitkan dengan ketidakcocokan antara kromosom, gonad dan fenotip. Sindrom Swyer adalah kelainan berupa disgenesis gonad 46,XY dan diikuti dengan tidak terjadinya pelepasan anti-mullerian hormone (AMH dari gonad yang mengalami disgenesis sehingga mengakibatkan berkembangnya duktus muller menjadi uterus. Keadaan tersebut mengakibatkan pasien mengeluh amenorea primer dan organ seks sekunder tidak berkembang. Orientasi gender umumnya adalah sebagai perempuan, karena kurangnya paparan hormon androgen terhadap otak. Tatalaksana kasus Disorders of sex development (DSD sangat mementingkan pasien (patient-centered sehingga tatalaksana medikamentosa maupun pembedahan harus berdasarkan pemahaman pasien terhadap orientasi gendernya. Pada makalah ini dilaporkan kasus sindrom swyer pada seorang perempuan usia 29 tahun dengan keluhan amenorea primer. Hasil analisis kromosom 46,XY (20 metafase dengan hormon follicle stimulating hormone (FSH 31.5miu/ml, luteinizing hormone (LH 10.8miu/ml, estradiol (E2 <5pg/ml, testosteron total (T <0.0025ng/ml. Tatalaksana medikamentosa adalah memberikan terapi hormon estrogen untuk membantu proses feminisasi. Karena risiko tinggi terjadinya tumor pada kasus disgenesis gonad intra-abdomen dengan kromosom Y, maka direncanakan tindakan gonadektomi. Kata kunci: Tatalaksana, Sindrom Swyer, pure gonadal dysgenesis Case Management of Pure Gonadal Dysgenesis 46, XY (Sindrom Swyer Abstract Disorders of sex development (DSD are medical conditions in which the development of chromosomal, gonadal or anatomic sex varies from normal and may be incongruent with each other. Swyer syndrome is a condition caused by pure gonadal dysgenesis 46,XY, which followed by inadequate anti-mullerian (AMH production results in maintenance and further development of mullerian duct into uterus. Therefore, many patients who suffer from this condition initially come with chief complaint of primary amenorrhea with insufficient development of secondary sexual characteristics. Patient’s gender orientation commonly is a female, since her brain was not sufficiently exposed with androgen hormone. Management of DSD patients should be based on patient-centered approach. Therefore, overall management of DSD patients should follow according to patient’s perception regarding to her gender orientation. Herein, we reported a case of Swyer syndrome in female aged 29 years with chief complain primary amenorrhea. Chromosomal analysis was 46,XY (20 mataphase, FSH level was 31.5miu/ml, LH 10.8miu/ml, estradiol (E2 <5pg/ml, testosteron total (T <0.0025ng/ml. Medical management for this patient has the purpose to drive her feminization process in order to improve her physical appearance. However, since there is a great tisk for having gonadal tumor development from intra-abdominal dysgenetic gonad with Y chromosome, this patient has been strongly suggested to have bilateral gonadectomy. Keywords: Management, Swyer syndrome, pure gonadal dysgenesis Normal 0 false false false IN X-NONE X-NONE Analisis Tata Kelola Pajak Bumi Bangunan Perdesaan Dan Perkotaan Di Kabupaten Bolaang Mongondow

    OpenAIRE

    Monoarfa, Lydiastuti; Karamoy, Herman; Ilat, Ventje

    2017-01-01

    . The property taxes in rural and urban areas of tax collection (PBB-P2) are delegated to local government by the Indonesian government. This study aims to analize how the governance of PBB-P2 in Bolaang Mongondow regency. This study aims to analyze how the governance of PBB-P2 in Bolaang Mongondow regency. This study is a qualitative research by using descriptive phenomenology and the Field of Tax and Retribution in DPKKAD as the research object. Data were obtained by in-depth interviews, ob...

  4. Tata Laksana Kasus Perempuan dengan Pure Gonadal Dysgenesis 46, XY (Sindrom Swyer)

    OpenAIRE

    Kanadi Sumapradja; Mila Maidarti; Achmad K. Harzif; Budi Wiweko; Gita Pratama; Muharam Natadisastra; Andon Hestiantoro

    2016-01-01

    Disorders of sex development (DSD) adalah kelainan medis yang dikaitkan dengan ketidakcocokan antara kromosom, gonad dan fenotip. Sindrom Swyer adalah kelainan berupa disgenesis gonad 46,XY dan diikuti dengan tidak terjadinya pelepasan anti-mullerian hormone (AMH) dari gonad yang mengalami disgenesis sehingga mengakibatkan berkembangnya duktus muller menjadi uterus. Keadaan tersebut mengakibatkan pasien mengeluh amenorea primer dan organ seks sekunder tidak berkembang. Orientasi gender umumn...

  5. STRATEGI PENGGUNAAN KOSAKATA DAN TATA BAHASA DALAM BERITA HARIAN UMUM INDEPENDEN SINGGALANG

    Directory of Open Access Journals (Sweden)

    Dina Ramadhanti

    2015-04-01

    Full Text Available This article  aims to describe the strategy of the use of vocabulary and grammer in general daily news i Singgalang newspaper. The method used is  descriptive method to descripe the use of vocabulary and grammer in writing news writer. The  discusiion carried out in accordance with the opinion expressed by Roger Flower, etal. Vocabulary  and grammer in general daily news in singgalang newspaper tend to marginalize the victim because writers to write descriptions of the perpetrator, while the victim semed to be ignored. Vocabulary used tend vocabulary and grammer marginalizations tend syntax used passive or passivation and nominalition resulting in the removal of the perpetrator in the news. Victims tend to be the main core of the problem

  6. Desain Tata Letak Sirkuit Terpadu sebagai Hak Kekayaan Intelektual dalam Hukum Indonesia

    Directory of Open Access Journals (Sweden)

    Sanusi Bintang

    2018-04-01

    Design of integrated circuits as blue prints for integrated circuit used in various products of information technology, such as computer, cellular phone, and telecomunication media, has its own characteristics which is not fit to be put under the previous intellectual property law regimes, therefore, it needs to be regulate under a specific legislation (sui generis. This research utilizes doctrinal legal research method by applying statute approach and comparative approach. Indonesia has enacted laws on Design of Integrated Circuits, but the law has certain limitations. The limitations is regarding the adequacy of subject matter, the quality of norms, and the legal enforcement. The limitations is not only from the aspect of technical legal drafting, but also rooted on legal culture.

  7. Kajian Perencanaan Tata Ruang untuk Memfasilitasi Kegiatan Non-Pertanian di Kecamatan Sukorejo, Kabupaten Kendal

    OpenAIRE

    Ratika Tulus Wahyuhana; Agung Sugiri

    2014-01-01

    Rural non-farm sector is potential to alleviate poverty and improve rural people’s welfare; however, its development is less encouraged by the government policy, especially the regional spatial plans in Indonesia (Sugiri, et al. 2011). The situation also occurs in Sukorejo District (Kecamatan) of Kendal Regency (Kabupaten) as a potential region for developing rural non-farm sector. This study is aimed at answering the research question of: "How can spatial planning facilitate better the devel...

  8. Evaluasi Tata Kelola Sistem Informasi Business Intelligence pada Perusahaan Minuman Ringan

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    Yanis Aulia Rizki

    2016-12-01

    Full Text Available Abstract: Implementation of Business Intelligence (BI can fulfill complete information needs, qualified, and suitable for each business unit levels to improve the quality of management decision-making. This study aimed to evaluate the governance of BI on one of soft drinks company in Indonesia (PMRI. The evaluation consists of investigation resources information system with descriptive qualitative method, analysis level of maturity by using maturity models COBIT framework 4.1 and analysis of system quality, information quality and service quality which the variable adapted from DeLone and McClane Model and analyzed with Importance Performance Analyst (IPA analysis. Based on the results of investigation resources information system already supports the implementation of BI, but it needs to improve. Based on the result of overall measurement maturity process is already followed the correct rules with COBIT framework and level Maturity is between level: 2 - Repeatable and level: 4 - Managed. Furthermore, based on IPA analysis, low-performance quality system and become priorities for the improvement of PMRI is reliability, accessibility, and efficiency, and for the low performance in the quality of information is attributed accuracy and timeliness, and for the low performance in the quality of service is an attribute of reliability and responsiveness. Improvements focus on enhancement of BI performance, planning, and management. Keywords: Soft Drinks Company, IT Governance, Business Intelligence, COBIT Framework 4.1, Maturity Level, Importance-Performance Analysis (IPA

  9. PENGARUH TATA RUANG DAN KOMUNIKASI INTERN TERHADAP EFEKTIVITAS KERJA GURU DI SMK NEGERI 9 SEMARANG

    Directory of Open Access Journals (Sweden)

    Ade Rustiana

    2016-01-01

    Full Text Available Office layout is a method to formulate spatial, tools, layout, and their physical environment that aims to provide a means for teachers and internal communications are an important means to unify the direction and the views and thoughts among teachers and between teachers with the Head of School, so create cohesion in the work. This research aims: 1 to determine whether there is any spatial effect on the effectiveness of teachers 'work; 2 to determine whether there is an internal communication influence the effectiveness of teachers' work; 3 to know how big influence of spatial planning and internal communication on the effectiveness of working collectively same. The population that became the subject of this study was the teacher of SMK Negeri 9 Semarang all 44 people who made the research sample because this study represents a population study. In this study, the independent variables are spatial and internal communications, while the dependent variable is the employment effectiveness of teachers. Data collection was performed using a questionnaire technique, documentation and observation. Data analysis technique used is descriptive analysis percentages, multiple regression analysis, simultaneous test, partial test, coefficient of determination and classical assumption. Based on the results of regression analysis obtained F value of 13.357 with a probability of 0.000 <0.05, which means that there is significant influence between spatial planning and internal communication on the effectiveness of teacher working SMK Negeri 9 Semarang. The amount of influence between spatial planning and internal communication on the effectiveness of teacher working SMK Negeri 9 for 39.5% of Semarang. Based on this research, the authors suggest that: in drafting desk should be more loose and in placing the furniture and other equipment should be to take advantage of the space available, so the staff room and classrooms and teachers more impressed with a neat move freely when doing academic activities; head schools should pay more attention to another teacher performance by giving instructions clearly did not even hesitate to reprimand if proven negligent in completing the job, as an effort to improve the quality of education, school principals should be willing to open ourselves to receive all complaints, ideas, or ideas from teachers.

  10. PENGARUH GAYA KEPEMIMPINAN, BUDAYA ORGANISASI, DAN TATA RUANG KANTOR TERHADAP KINERJA PEGAWAI

    Directory of Open Access Journals (Sweden)

    Melinda Puspitasari

    2018-03-01

    Full Text Available The purpose of this study is to determine whether there is influence of archival management and competence on the quality of archival services in the Department of Education and Culture of Demak Regency. The population in this study was all employees in DinsosP2PA Demak which were 76 employees with saturation sampling technique. Data collection methods used in this study were questionnaires, observation, documentation, and interviews. Data analysis using multiple linier regression analysis method, classical test assumption analysis, descriptive percentage analysis, and hypothesis test analysis with SPSS For Windows versi 21.0 program. The results of multiple linear regression analysis obtained equation Y = -3,880 + 0,434 X1 + 0,292 X2 + 0,219 X3. The significance test of regression equation with F test, obtained Fcount = 29,520 with significance 0,000 and less than 0,05. The influence of simultaneous or joint from the leadership style, organizational culture, and office layout towards the employee performance is equal to 53,3%.

  11. Tata Kelola Pada Perguruan Tinggi Menggunakan Control Objective for Information & Related Technology (COBIT) 5

    OpenAIRE

    asriyanik, asriyanik; Hendayun, Mokhamad

    2017-01-01

    A College needs a good governance in implementation of information technology, especially which related with information security because information is an important asset. Framework which can be used for the preparation of information security governance is COBIT 5 from ISACA. The first step to start the preparation of the governance using COBIT is mapping between the goal of college with enterprise goals (EG), IT Related Goals and domain process COBIT 5. From 15 colleges that have been acc...

  12. STRATEGI SISTEM AUDIT INTERNAL PEMERINTAH DALAM RANGKA TATA KELOLA YANG BAIK

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    Maliki Heru Santosa

    2016-10-01

    Full Text Available The problems related to governance system such as management irregularities or fraud, unaccountable finan-cial practices, and poor public service deliveries have raised some questions to the effectiveness of Units of Government Internal Audit (GIA. The complexity of role and function of GIA, which are both directly and indirectly influenced by the stakeholders and relevant environment, need an appropriate research using sys-temic context. The objective of this study was to identify and establish priority strategy in developing effective internal audit system. This research was based on qualitative approaches with systems methodology which was started with literature study, in-depth interview and focus group discussion (FGD. Then, the study was continued with analytical hierarchy process (AHP with experts representing the stakeholders. The study showed that to enhance the effectiveness of GIA, it is important to have priority strategy to strengthen the governance system, risk management, and control. The role of the stakeholders and management maturity are the key requirements to successful and an effective of GIA System.

  13. Analisis Tata Kelola Teknologi Informasi Menggunakan Kerangka Kerja COBIT 5 Pada AMIK JTC Semarang

    OpenAIRE

    Hilmawan, Hadi; Nurhayati, Oky Dwi; Windasari, Ike Pertiwi

    2015-01-01

    Informations technology has been important elements in an organisation but getting higher information technology implementation cost requires any company or organization to implement an effective operation of information technology. Information technology was expected to help organization in achieveing their business goals and providing not only financial profit but also non-financial profit. To achieve their business goals and ensuring process of information technology works effectively and ...

  14. Analisis Tata Kelola Teknologi Informasi menggunakan Kerangka Kerja COBIT 5 pada AMIK JTC Semarang

    OpenAIRE

    Hadi Hilmawan; Oky Dwi Nurhayati; Ike Pertiwi Windasari

    2015-01-01

    Informations technology has been important elements in an organisation but getting higher information technology implementation cost requires any company or organization to implement an effective operation of information technology. Information technology was expected to help organization in achieveing their business goals and providing not only financial profit but also non-financial profit. To achieve their business goals and ensuring process of information technology works effectively and ...

  15. Pre and Post Merger Valuation- A Study of Tata Corus Merger Deal

    OpenAIRE

    Sumeet Gupta

    2009-01-01

    Indian Economy being characterized as Global Economy leading to inorganic growth through Mergers, Acquisitions, Takeovers and Joint Ventures. Mergers would affect the company’s inflow and outflow of various variables like sales, expenses and cash position etc. It becomes imperative to evaluate the pre merger and post merger conditions of the firms so that successful deal can be carried out. The deal would also affect the shareholders wealth. The prediction of future forecasted sales and other...

  16. Pengaruh Tata Kelola Perusahaan dan Penghindaran Pajak Terhadap Manajemen Laba Pada Perusahaan Manufaktur di Indonesia

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    Juoro Larastomo

    2016-05-01

    Full Text Available The Impact of Corporate Governance and Tax Avoidance on Earning Management at Indonesian Manufacturing CompaniesThe objective of this study is to determine the impact of corporate governance and tax avoidance on earning management by Indonesian manufacturing companies listed on IDX in 2010-204. This study used board of commissioner, independent commissioner, audit committee, and managerial ownership as corporate governance’s variabel. Population of this study is Indonesian manufacturing companies listed on IDX in 2010-2014. Sample of this study are 21 companies that generated using purposive sampling method. The multiple regression analysis test showed that board of commissioner, independent commissioner and tax avoidance have positive impact on earning management. But, managerial ownership has positive impact on earning management and audit committee has no impact on earning managementDOI: 10.15408/ess.v6i1.3121

  17. Pedoman Tata Kelola Teknologi Informasi Menggunakan It Governance Design Frame Work (Cobit Pada PT. X

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    I Ketut Adi Purnawan

    2015-12-01

    Full Text Available Implementation of  Information Technology (IT in an organization require significant costs with high risk  of  failure [3]. Managing data is  a matter that must be done continuously by the organization and accompanied by monitoring and measurement of achievement that has been done as to meet the aspect of integrity, availablility. In this study using COBIT as a frame work in preparing the guidelines for information technology governance at PT.  X  on  DS11,  which  focuses  on  management of  data  about  the  level  of  concern  for management (management awareness and  maturity level (maturity level.  The study and analysis indicates that the level of concern for management (management awareness PT. X already on a fairly level and maturity level for the current maturity level (as is at level 3 (defined process and to the expected level of maturity located at level 5 (optimized. From the overall study results showed that PT. X has recognized that the data is an important organizational asset.

  18. LINGKUNGAN "PECINAN" DALAM TATA RUANG KOTA DI JAWA PADA MASA KOLONIAL

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    Handinoto Handinoto

    1999-01-01

    Full Text Available Pecinan (Chinese Camp area is never absent in the town of Java. Although the specific characteristics of this mileu is not so strong any more at the present as it was in the past, its presency in diverse smaller towns in Java is still felt as something different. The specific atmosphere of the area, centered on the klenteng as the place of workship, its social environment, included the specific style of house construction, are easy to be recognized. In some world cities like San Fransisco and Manila, the socalled China Towns are just stimulated for its existence. It is even so far, that theyare recomended as tourist destination objects. During the rule of the New Order (1965-1998, Pecinan in the towns in Indonesia are systimatically abolished, because of sicio political considerations. This paper tries to trace back the history of those Chinese Camps in the older towns of Java, to have a certain picture of its existence in the past. Abstract in Bahasa Indonesia : Lingkungan "Pecinan" selalu ada di hampir semua kota-kota di Jawa. Meskipun sekarang lingkungan ini sudah semakin kabur, tapi di beberapa kota kecil di Jawa bekas kehadirannya masih sangat terasa sekali. Atmosfir lingkungannya yang khas, diperkuat dengan kehadiran kelenteng sebagai pusat ibadah dan sosial, serta bentuk-bentuk bangunan yang khas pula sangat mudah untuk ditengarai. Di beberapa kota di dunia seperti San Fransisco, Manila dan sebagainya daerah Pecinan ini justru di perkuat kehadirannya. Bahkan daerah tersebut bisa dijadikan sebagai daerah tujuan wisata kota. Selama Orde baru, karena alasan sosial dan politik, kehadiran Pecinan di kota-kota Indonesia, mulai dihapuskan. Tulisan ini mencoba untuk menelusuri sejarah kehadiran daerah "Pecinan" pada kota-kota di Jawa pada masa lampau. Kata kunci : Kota di Jawa jaman kolonial, Pecinan.

  19. TATA LETAK GUDANG RAW MATERIAL CHEMICAL MENGGUNAKAN METODE SHARED STORAGE DAN REL SPACE

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    Indramawan Hadi Kuswoyo

    2016-04-01

    Full Text Available Storage system in the manufacturing industry in this modern era is a very important role especially international scale company, may not directly produce or distribute all of the work unit. this led to the need for raw materials warehouse, warehouse storage systems should not be large in size because if supported by a good inventory of the warehouse to the maximum utilization of the problems faced by the company occurred in the warehouse of raw materials (raw materials. deficiencies in the arrangement of items in the warehouse procedures cause problems in the warehouse, so the warehouse impressed narrow and less structured cause inefficiencies time retrieval and storage of materials, as well as complicate the operator in handling the placement process raw materials. In the method of shared storage and rail space (relationchip chart for the relationship between activity is indicated by activity relationship approach, which shows each activity as a single activity model in the form of a diagram. ARD basic idea of the link between patterns of flow of goods and location of service activities related to production activities. ARD is the development of ARC (activity relationship chart.

  1. Rancang Bangun Tata Kelola Kinerja Perguruan Tinggi Menggunakan IT Balanced Scorecard

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    Achmad Solechan

    2016-05-01

    Full Text Available Budgeting most colleges are not based on good governance and conceptual, but based on short-term needs in each college. Other problems are related to the use of information technology in both hardware, software and brain ware (user in the management of information technology is still not well-thought in its development. This research aims to design a wake-up governance by using the Information Technology IT Balanced Scorecard framework is a system of management, measurement and control quickly, accurately and comprehensively can provide insight to the Leadership of the portrait and the development of information technology in institution. Information system development using SDLC (system development life cycle. College performance measurement method using IT balanced scorecard framework. The results showed that the use of information systems governance college performance using IT Balanced Scorecard help the efficiency and effectiveness in making an evaluation report IT governance, helping the presentation of information and error reduction calculations, the transaction data maintained intact his security, was able to complete the reporting process with fast, speed up the calculation process and the conclusion of the assessment of the performance of the college.

  2. Jejak Kuasa Atas Tata Ruang (Studi Kasus Kebijakan Revitalisasi Hutan Kota Malabar

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    H.B. Habibi Subandi

    2017-10-01

    Full Text Available Problem revitalisasi hutan kota dan upaya penyediaan Ruang Terbuka Hijau (RTH oleh Pemerintah di wilayah perkotaan pada dasarnya merupakan satu kajian baru di bidang politik lingkungan di Indonesia. Penelitian ini secara khusus mengkaji kebijakan revitalisasi Hutan Kota Malabar di Kota Malang dengan pisau analisis anthroposentrisme dan ekosentrisme. Dengan menggunakan metode focus group discussion (FGD dan dokumentasi, penulis melacak jejak kuasa dalam kebijakan lingkungan di wilayah-wilayah perkotaan. Berdasarkan analisis fakta di lapangan ditemukan sebuah trade-off kepentingan dalam skema kebijakan revitalisasi hutan kota Malabar tahun 2015. Penggunaan dana Tanggung Jawab Sosial Lingkungan (TJSL dengan skema build-transfer-operate (BTO dalam revitalisasi hutan menunjukkan terjadinya penetrasi modal di balik inisiatif penyediaan RTH yang melibatkan pihak swasta di Kota Malang. Di satu sisi, kebijakan ini dapat mempermudah pemerintah dalam pembangunan RTH. Namun di sisi lain kebijakan ini dapat merugikan anggaran pemerintah yang hanya berfungsi sebagai operator RTH.

  3. Analisis Tata Kelola Teknologi Informasi menggunakan Kerangka Kerja COBIT 5 pada AMIK JTC Semarang

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    Hadi Hilmawan

    2015-04-01

    Full Text Available Informations technology has been important elements in an organisation but getting higher information technology implementation cost requires any company or organization to implement an effective operation of information technology. Information technology was expected to help organization in achieveing their business goals and providing not only financial profit but also non-financial profit. To achieve their business goals and ensuring process of information technology works effectively and efficiently, a continuously audit and evaluation of our IT process need to introduced in organization. It will help organization aligning their business process with IT process. One of IT governance framework is COBIT 5. COBIT 5 is a comprehensive framewok that helps organizations to create optimal value from IT by maintaining a balance between realizing benefits and optimizing risk levels and resources use. COBIT 5 enables information and related technology to be governed and managed in a holistic manner for the whole organizations, taking full end to end business and functional area of responsibility, considering the IT-related intererst of internal and external stakeholders.

  4. Penerapan Pakta Integritas pada Pengadaan Barang/Jasa untuk Mewujudkan Tata Kelola Pemerintah yang Bersih

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    Muhammad Insa Ansari

    2016-12-01

    ABSTRACT. Integrity Pact is an affidavit which contains a pledge to prevent and not collusion, corruption and nepotism in the Procurement of Goods / Services. Integrity Pact on Procurement of Goods / Services introduced in Presidential Decree Number 80 Year 2003 concerning Procurement of Government Goods / Services. After the Presidential Decree Number 80 Year 2003 concerning Procurement of Government Goods / Services revoked by Indonesian Presidential Regulation Number 54 Year 2010 concerning Procurement of Government Goods / Services, the integrity pact is still maintained in the presidential decree. Setting the integrity pact in procurement of government goods / services is one of the efforts to achieve good governance and clean government. But more important is the application of the material and does not pledge to prevent collusion, corruption and nepotism as stated in the integrity pact. The implementation of integrity pacts in procurement of government goods / services is one of the government's efforts in order to realize Good Governance and Clean Government.

  5. Penyusunan Tata Kelola Teknologi Informasi Bagi Pemerintah Daerah Menggunakan Framework Cobit 5 (Studi Kasus: Kabupaten Cilacap

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    Annas Wahyu Purwanto

    2013-12-01

    ABSTRACT The failure of the e -government implementation in Indonesia according to Junseok Hwang and Irfan Syamsudin, who were doing research in South Sulawesi, are from the government, not from the people.  Meanwhile in Cilacap District,  Long Term Development Plan for the period 2005-2025 mentioned that e-government in Cilacap already implemented but not optimal yet. Cole methodology used in this study consists of 4 (four stages: problem identification , intervention , evaluation and reflection and learning. Conduct a gap analysis and risk analysis to determine the priorities that need to be carried out in Cilacap Kabupaten. To pursue a level of capability gaps that occur and mitigate the risks that may arise, Cilacap necessary to establish an organizational structure of information technology (IT, convey the purpose and management direction to stakeholders, understanding the direction of government development, develop a vision of the IT architecture governance, maintain the availability of the IT staff, identify IT personnel key, maintain the skills and competency of IT personnel, evaluate the performance achievement of IT personnel and also planning  and mapping of IT human resources .

  6. PRAKTIK TATA KELOLA PERUSAHAAN (CORPORATE GOVERNANCE DAN USEFULNESS INFORMASI AKUNTANSI (Telaah Teoritis Dan Empiris

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    Muhammad Miqdad

    2012-01-01

    Full Text Available Accounting researchs on corporate governance and earnings management refers to the positive accounting theory and agency theory. Most of the result empirical studies showed that implementing of good corporate governance (GCG negatively affect to earnings management. Excessive earnings management practices will affect the credibility of the financial statements in the user’s perception namely investors, creditors and other stakeholders. Corporate governance and earnings management associated with behavioral problems, therefore qualitative research method as an alternative to explore further of the behavioral problems.

  7. Academic Integrity and Plagiarism Prevention at Tata Institute of Social Sciences, Mumbai: A case Study

    OpenAIRE

    Bandi, Shekappa; Pothare, Devyani; Angadi, Mallikarjun; Jange, Suresh

    2016-01-01

    Plagiarism is not always a black and white issue. The boundary between plagiarism and research is often unclear. Learning to recognize the various forms of plagiarism, especially the more ambiguous ones, is an important step towards effective prevention. The study overview the concept and types of plagiarism and it`s benefits, Plagiarism Policies in India, and also discussed turnitin and its workflow process of the TISS comparison of the Turnitin and iThenticate plagiarism tools and other rel...

  8. PENENTU BESARAN TRANSAKSI PIHAK BERELASI: TATA KELOLA, TINGKAT PENGUNGKAPAN, DAN STRUKTUR KEPEMILIKAN

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    Cynthia Afriani Utama

    2015-06-01

    Full Text Available Indonesian firms are characterized by conglomeration that tends to conduct related party transaction (RPT. Extant academic literature provides two competing views on RPTs: the efficient transaction hypothesis and the conflict of interest hypothesis. The purpose of this study is to investigate RPT from the point of view of the conflict of interest hypothesis. Specifically, this study examines the size of RPT which is performed by majority shareholders to expropriate minority shareholders. The size of RPT measures the direct influence of RPT on shareholders’ wealth. In this study, the size of RPT is measured by RPT transactions of assets plus liabilities (RPTAL and sales plus expenses (RPTSE relative to book value of equity. Furthermore, this study investigates whether RPTAL and RPSE are determined by CG practices, disclosure of RPT, and ownership structure. This study cannot find the influence of CG on size of RPTAL and RPTSE. The results of the study also show that only disclosure of RPT and ownership structure that have positive impact on size of RPTSE. Disclosure of RPT increases more efficient RPTSE than abusive RPTSE. This study find that the relationship between the disclosure and RPTAL is insignificant as efficient RPTAL does not dominate abusive RPTAL, while concentrated ownership has a positive impact on abusive RPTSE.

  9. Perwujudan/Implementasi Konsep Interaksi Aerotropolis Berbasis Tata Ruang di Indonesia

    OpenAIRE

    Jermanto Setia Kurniawan

    2016-01-01

    Banyak kegiatan USAha atau jasa komersial di sekitar kawasan bandar udara menjadi dasar terbentuknya konsep/model kota bandara (airport city) yang merupakan embrio terbentuknya konsep aerotropolis. Konsep ini berkembang secara organik dan dimulai dari bandar udara itu sendiri, dimana bandar udara tak hanya sebatas sebagai pendukung kegiatan penerbangan. Lebih dari itu, ada berbagai jenis kegiatan bisnis lainnya, sehingga bandar udara dan wilayah sekitarnya merupakan kawasan komersial yang ter...

  10. PEMODELAN BANGKITAN PERGERAKAN PADA TATA GUNA LAHAN SEKOLAH DASAR SWASTA DI SURABAYA

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    Harry Patmadjaja

    2002-01-01

    Full Text Available Elementary School is a common location for traffic congestion especially every morning. This condition can be avoided if the elementary school planner can predict the trip generation of that school. The aim of this research is to make a mathematical model of trip generation for elementary school land use at Surabaya. Research has been done to eight private elementary school using Pearson Correlation Analysis, Simple Regression Analysis, and Stepwise Regression Analysis to obtain the most significant trip generation model. Arrival rate and service rate distribution was analyzed using Chi-Square Distribution Fitting method to obtain arrival rate and service rate characteristic. From the analysis, the most significant trip attraction model is Y = -867,9 + 194.Log X1 + 274.Log X8 + 177.Log X5, in which Y is number of student vehicle, X1 is number of elementary student, X8 is total area of classroom, and X5 is classroom capacity. The most significant trip production model is Y= -797,2 + 311.Log X1 + 164.Log X8 +79.Log X15, in which Y is number of student vehicle, X1 is number of elementary student, X8 is total area of classroom, and X15 is ratio between number of teacher and classroom. Several elementary school characteristics that influence traffic congestion are: number of student, number of queue lane, number of entrance, and vehicle occupancy. Abstract in Bahasa Indonesia : Sekolah dasar merupakan salah satu lokasi yang sering mengalami masalah kemacetan lalulintas. Hal tersebut bisa dicegah bila pemilihan lokasi sekolah dilakukan dengan mempertimbangkan perkiraan bangkitan pergerakan yang akan terjadi. Tujuan dari penelitian ini adalah untuk membuat model bangkitan pergerakan (trip generation kendaraan roda empat (pribadi dan antar jemput akibat pembangunan sekolah dasar swasta di Surabaya. Survey dilakukan pada delapan sekolah dasar swasta di Surabaya. Hasil survey dianalisis dengan metode Pearson Correlation, Simple Linear Regression, dan Stepwise Regression untuk mendapatkan model persamaan matematis yang paling signifikan. Distribusi laju kedatangan dan laju pelayanan dianalisa dengan metode chi square distribution fitting untuk mengetahui karakteristik laju kedatangan dan laju pelayanan kendaraan pengantar siswa. Hasil analisa menunjukkan model terbaik untuk meramalkan pergerakan yang tertarik menuju ke sekolah dasar swasta (trip attraction adalah Y = -867,9 + 194.Log X1 + 274.Log X8 + 177.Log X5, dimana Y adalah jumlah kendaraan pengantar, X1 adalah jumlah siswa sekolah dasar, X8 adalah luas total kelas, dan X5 adalah kapasitas kelas. Sedangkan model terbaik untuk meramalkan pergerakan yang berasal dari sekolah dasar swasta (trip production adalah Y= -797,2 + 311.Log X1 + 164.Log X8 +79.Log X15, dimana Y adalah jumlah kendaraan penjemput, X1 adalah jumlah siswa sekolah dasar, X8 adalah luas total kelas, dan X15 adalah jumlah guru dibagi jumlah kelas. Beberapa karakteristik sekolah dasar yang mempengaruhi kelancaran lalulintas adalah: jumlah siswa, jumlah lajur antrian, jumlah pintu masuk, dan okupansi kendaraan.

  11. PENGARUH PENERAPAN E-GOVERNMENT TERHADAP PELAKSANAAN TATA KELOLA PEMERINTAH DI PEMERINTAH KABUPATEN CIANJUR

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    Toni Heryana

    2013-04-01

    Full Text Available This research has purposes to know how the implementation of e-government, implementation of the Government Governance and e-government influence on the implementation of the Government Governance in the Government of Cianjur. The population in this research is 58 (Fifty eight OPD in the Government environment Cianjur. Based on purposive sampling is then used 26 (twenty six OPD in the Government environment of Cianjur. Data collection was done through the dissemination of a questionnaire using the semantic defferential scale. To test the effect of e-government (X with respect to the implementation of the governance of the Government (Y, then in this study used a simple linear regression. The final results of the research done indicates that the implementation of e-government on most of the Department, Agencies and Offices in Cianjur influence of 78,5% with respect to the implementation of the governance of the Department, Agencies and Offices in Cianjur. While the rest 21,5% is the influence of another factors outside of e-government.

  12. Hubungan Pengetahuan Gizi dengan Konsumsi Kfc Mahasiswa Pendidikan Tata Boga Universitas Negeri Padang

    OpenAIRE

    Lindra, Maifo; Fridayati, Lucy; Holinesti, Rahmi

    2016-01-01

    This study was aimed to describe nutrition a knowledge and KFC consumption and analyze the relation between nutrition a knowledge with KFC consumption Students Culinary Education UNP. This study was a correlational, with a population in this research was 151 people, and all of them sample. The data were collection techniques for nutrition a knowledge by using a questionnaire enclosed in the form multiple choise as well as KFC consumption by using open questionnaire in the form essays. The res...

  13. BIMBINGAN KELOMPOK BERBASIS TATA NILAI BUDAYA LAMPUNG UNTUK MENINGKATKAN TINGKAH LAKU PROSOSIAL SISWA

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    Nita Fitria

    2015-10-01

    Full Text Available AbstractProsocial behavior is a concern which is described by the willingness to help others, empathy and generosity. To develop this attitude, it needsguidance and counseling service with a strong social values and culture. This study aims to know the effectiveness of group guidance based on Lampung cultural values to increase the prosocial behavior. These samples included 15 that randomized from 76 students. The research method that writer used is the pre-experimental designs. Based on the data analysis of prosocial behavior scores before of the students following group guidance based on Lampung value cultural services in 1929, then after following the service the scores increased to 2631. So this means that Null Hypothesis (Ho is rejected and Alternative Hypothesis (Ha is accepted. It can be included that the group guidance model based on Lampung cultural values is effective to improve the student prosocial behavior.  Keyword : group guidance, prosocial behavior, cultural values 

  14. PENGARUH LIPUTAN MEDIA, KEPEKAAN INDUSTRI, DAN STRUKTUR TATA KELOLA PERUSAHAAN TERHADAP KUALITAS PENGUNGKAPAN LINGKUNGAN

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    Badingatus Solikhah

    2016-06-01

    Full Text Available This study aims to examine the influence of media coverage, industry sensitivity and corporate governance structure on the quality of environmental disclosure. The sample of this study is taken from six industries (mining, energy, chemicals, pharmaceuticals, cosmetics, food and beverages from Indonesia Stock Exchange during 2011, 2012 and 2013. This study uses panel data regression analysis and employs 128 listed companies as the samples of the study. The results show that industry sensitivity, multiple directorship, board size, firm size and ROA positively affect the quality of environmental disclosure. The results also show a negative effect of independent commissionaire on environmental disclosure. This study also provides evidence that media, gender and institutional ownership have no effect on environmental disclosure.

  15. Penerapan Aplikasi Fruity Loops sebagai Media Pembelajaran Penciptaan Komposisi dan Aransemen Tata Suara

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    Yunanto Tri Laksono

    2017-11-01

    Full Text Available The software is one of the essential tools in the learning process used in the modern era today, both for the improvement of technology and as a medium of learning. Fruity Loops is one of the learning media based on digital technology in the form of software used in writing and creating works or arrangements on the art of music in the way of partitions and audio. The structure of the creation of compositions and arrangements is an essential part of the art of music, mostly the value of harmonization created and felt in developing creativity in the art of music. Several things play a crucial role in the planning of the arrangement and composition, namely: (a the form of musical composition, (b primary ideas, (c concepts, (d patterns and methods of arrangement, (e musical works. DIV Computer Multimedia at Stikom Surabaya which is the object of this research is one of the study programs which focus on film, television, and animation, so the purpose of learning through Fruity Loops is to develop the concept of sound in animation and film, and videos are original and do not violate copyright ethics, and serve as an introduction to learning based on Communication Technology.

  16. Pendidikan Tata Rias Pengantin (Studi Situs Pengelolaan pada LKP Moncar Surakarta

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    Wahyu Ari Indriastuti

    2015-07-01

    Full Text Available This research aimed to describe characteristics of (1 education planning; (2 education organizing; (3 education implementation; (4 education evaluation; and (5 supporting and inhibiting factors for education bridal makeup at LKP Mon- car. Type of research is a qualitative with an ethnographic approach. Implementation research in LKP Moncar Surakarta. Informants were chosen are the owner of LKP Moncar, learners, instructure. The validity of the data using source triangulation tech- niques and triangulation method. The conclusions of this research are characteristics of education bridal makeup in LKP Moncar Surakarta (1 education planning through ideas, acceptance of new students, and preparing educational bridal makeup materials are arranged in the form of Teaching Education (Unit Satuan Ajar Pendidikan/ SAP; education organizing done by placing instructors to handle incoming new students and handling facility management skills institutions, infrastructure, media learning and personnel and the distribution of skill levels of learners to all instructors and organize training agenda and organize training agenda and competition schedules based on each competition to the instructor; (3 education implementation in are procurement, usage, maintenance, and removal of owned skills institutions and achievement social- ization to new students also move instructors to carry out activities to achieve the target graduation; (4 education evaluation is done through planning and implementa- tion of admission new students and refers to skills attainment, graduation rates, and acquisition grades of student while monitoring the performance of team success; and (5 Factors supporting education at LKP Moncar is the support of family (husband and children, cost of teaching and learning needs is low, learning facilities bridal makeup does not always use the media and certain brands, and inhibiting factor for education is the difficulty of obtaining student.

  17. Eredoctoraat voor kapitalistische Ratan Tata schaadt geloofwaardigheid van UvA

    NARCIS (Netherlands)

    Bala, S.

    2013-01-01

    De UvA kent - in navolging van andere universitaire instellingen - een eredoctoraat toe aan de CEO van een megabedrijf dat zwaar wordt bekritiseerd omwille van haar steeds groeiende bedrijfsmonopolie. De Amsterdamse assistent-professor Sruti Bala en twintig collega's schreven een protestbrief.

  18. Selection of reference genes for expression analysis using quantitative real-time PCR in the pea aphid, Acyrthosiphon pisum (Harris (Hemiptera, Aphidiae.

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    Chunxiao Yang

    Full Text Available To facilitate gene expression study and obtain accurate qRT-PCR analysis, normalization relative to stable expressed housekeeping genes is required. In this study, expression profiles of 11 candidate reference genes, including actin (Actin, elongation factor 1 α (EF1A, TATA-box-binding protein (TATA, ribosomal protein L12 (RPL12, β-tubulin (Tubulin, NADH dehydrogenase (NADH, vacuolar-type H+-ATPase (v-ATPase, succinate dehydrogenase B (SDHB, 28S ribosomal RNA (28S, 16S ribosomal RNA (16S, and 18S ribosomal RNA (18S from the pea aphid Acyrthosiphon pisum, under different developmental stages and temperature conditions, were investigated. A total of four analytical tools, geNorm, Normfinder, BestKeeper, and the ΔCt method, were used to evaluate the suitability of these genes as endogenous controls. According to RefFinder, a web-based software tool which integrates all four above-mentioned algorithms to compare and rank the reference genes, SDHB, 16S, and NADH were the three most stable house-keeping genes under different developmental stages and temperatures. This work is intended to establish a standardized qRT-PCR protocol in pea aphid and serves as a starting point for the genomics and functional genomics research in this emerging insect model.

  19. Modelling the mechanical behaviour of heterogeneous Ta/TA6V welded joints: behaviour and failure criteria; Modelisation du comportement mecanique des liaisons soudees heterogenes Ta/TA6V: comportement et critere de rupture

    Energy Technology Data Exchange (ETDEWEB)

    Paris, Th

    2008-12-15

    As laser welding of two different materials (heterogeneous welding) leads to a joint having a characteristic size close to the millimetre, i.e. much smaller than that of a structure, and as such a junction displays completely different mechanical properties because of the metallurgical transformations induced by intense thermal loading, the aim of this research thesis is to develop a behaviour model, flexible and robust enough, to represent all together the mechanical behaviours of the Ta, the TA6V and the melted zone. This model must be able to take plasticity and visco-plasticity into account, and also to provide a failure criterion through damage mechanics and its coupling with the behaviour. The author first reports the experimental characterization of the base materials (Ta and TA6V) by using tensile tests under different strain rates and different directions, relaxation tests and fatigue shear tests. He also characterizes the melted zone by describing the influence of a thermal treatment (induced by welding) on the formation of the melted zone, and by using different tests: four point bending on notched specimens, nano-indentation test, and longitudinal tensile test. In a second part, the author develops the model within the framework of continuum thermodynamics, and explores the numerical issues. The last part deals with the validation of the model for the concerned materials (Ta and TA6V) and melted zone.

  20. CLUSTER MODEL FOR EXTENSIVE GIANT TIGER SHRIMP (Penaeus monodon Fab. TO PREVENT TRANSMISSION OF WHITE SPOT SYNDROME VIRUS

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    Arief Taslihan

    2015-06-01

    Full Text Available White spot syndrome virus (WSSV has become epidemic in Indonesia and affecting shrimp aquaculture interm of its production. White spot syndrome virus is transmitted from one to other ponds, through crustacean, included planktonic copepode as carrier for WSSV and through water from affected shrimp pond. A cluster model, consist of shrimp grow out ponds surrounded by non-shrimp pond as a role of biosecurity has been developed. The model aimed to prevent white spot virus transmission in extensive giant tiger shrimp pond. The study was conducted in two sites at Demak District, Central Java Province. As the treatment, a cluster consist of three shrimp ponds in site I, and two shrimp ponds in site II, each was surrounded by buffer ponds rearing only finfish. As the control, five extensive shrimp grow out ponds in site I and three shrimp grow out ponds in site II, with shrimp pond has neither applied biosecurity nor surrounded by non-shrimp pond as biosecurity as well considered as control ponds. The results found that treatment of cluster shrimp ponds surrounded by non-shrimp ponds could hold shrimp at duration of culture in the grow out pond (DOC 105.6±4.5 days significantly much longer than that of control that harvested at 60.9±16.0 days due to WSSV outbreak. Survival rate in trial ponds was 77.6±3.6%, significantly higher than that of control at 22.6±15.8%. Shrimp production in treatment ponds has total production of 425.1±146.6 kg/ha significantly higher than that of control that could only produced 54.5±47.6 kg/ha. Implementation of Better Management Practices (BMP by arranging shrimp ponds in cluster and surrounding by non-shrimp ponds proven effectively prevent WSSV transmission from traditional shrimp ponds in surrounding area.

  1. The development of a high density linkage map for black tiger shrimp (Penaeus monodon based on cSNPs.

    Directory of Open Access Journals (Sweden)

    Matthew Baranski

    Full Text Available Transcriptome sequencing using Illumina RNA-seq was performed on populations of black tiger shrimp from India. Samples were collected from (i four landing centres around the east coastline (EC of India, (ii survivors of a severe WSSV infection during pond culture (SUR and (iii the Andaman Islands (AI in the Bay of Bengal. Equal quantities of purified total RNA from homogenates of hepatopancreas, muscle, nervous tissue, intestinal tract, heart, gonad, gills, pleopod and lymphoid organs were combined to create AI, EC and SUR pools for RNA sequencing. De novo transcriptome assembly resulted in 136,223 contigs (minimum size 100 base pairs, bp with a total length 61 Mb, an average length of 446 bp and an average coverage of 163× across all pools. Approximately 16% of contigs were annotated with BLAST hit information and gene ontology annotations. A total of 473,620 putative SNPs/indels were identified. An Illumina iSelect genotyping array containing 6,000 SNPs was developed and used to genotype 1024 offspring belonging to seven full-sibling families. A total of 3959 SNPs were mapped to 44 linkage groups. The linkage groups consisted of between 16-129 and 13-130 markers, of length between 139-10.8 and 109.1-10.5 cM and with intervals averaging between 1.2 and 0.9 cM for the female and male maps respectively. The female map was 28% longer than the male map (4060 and 2917 cM respectively with a 1.6 higher recombination rate observed for female compared to male meioses. This approach has substantially increased expressed sequence and DNA marker resources for tiger shrimp and is a useful resource for QTL mapping and association studies for evolutionarily and commercially important traits.

  2. MANAGEMENT STRATEGY FOR SHRIMP (PENAEUS MONODON, FABRICIUS FARMING AT BHAGBANPUR REGION IN PURBA MEDINIPUR DISTRICT OF WEST BENGAL, INDIA

    Directory of Open Access Journals (Sweden)

    S. Sahu

    2013-06-01

    Full Text Available Shrimp culture can give miracles towards socio-economic development for a large number of rural people. Its sustainable development can be achieved through the application of scientific methodology, input of technical knowledge at the crisis time, quality control mechanism targeted to marketing strategy, sufficient supply of institutional credit and Government involvement in the marketing strategy. An optimum output cannot be assured even after taking the risk of huge investment. The export policy promotion and distinct quality measures are the major thrust area for the recent days. Government efforts in implementing a proper planning and management oriented marketing strategies are required for the sustainability of the industry.

  3. The potential effect of greenwater technology on water quality in the pond culture of Penaeus monodon Fabricius

    NARCIS (Netherlands)

    Tendencia, E.A.; Bosma, R.H.; Verdegem, M.C.J.; Verreth, J.A.J.

    2015-01-01

    Whitespot syndrome virus (WSSV) has caused severe production drops in the shrimp industry. Numerous scientific manuscripts deal with WSSV epidemiology, but reports on minimizing disease outbreaks through ecological means are rare. Industry stakeholders resorted to various innovative techniques to

  4. Improving the quality of tiger shrimp Penaeus monodon through dietary incorporation of algae as a source of natural pigment

    OpenAIRE

    Kunal Mondal; Subhra Bikash Bhattacharyya; Abhijit Mitra

    2015-01-01

    Tiger shrimp is one of the major candidate species for export oriented aquaculture which dominates the seafood market in regions of European Union, Japan and USA. Carotenoid content in seafood has now become one of the important criteria in determining the quality of edible product. Recent trends in supplementing fish diets with natural pigment source are an alternative to the utilization of expensive synthetic pigments. In this context, green algae Enteromorpha intestinalis was selected as a...

  5. The changes of astaxanthin content and chemical characteristics of tiger prawn (Penaeus monodon) due to processing: boiling, smoking and frying

    Science.gov (United States)

    Swastawati, F.

    2018-03-01

    Food processing using high temperatures can cause changes in pigment color and chemical characteristics in food stuffs, including prawn. The aim of this research was to evaluate the changes in pigment and chemical characteristics of tiger prawn caused by boiling, smoking and frying. Ten kg of tiger prawn was boiled, smoked and fried at the temperature of ± 100 °C for ± 10 min. The results showed that boiling, smoking and frying gave a significant effect (P < 0.05) on the astaxanthin pigment, pH, moisture, protein, salt content, Aw and color. The content of astaxanthin pigments in fresh prawn, boiled prawn, smoked prawn and fried prawn was: 132.79 ± 1.5 μg·g-1 82.89 ± 0.92 μg·g-1 78.28 ± 0.1 μg·g-1 and 91.35 ± 2.59 μg·g-1, respectively. The value of °Hue on fresh prawn, boiled prawn, smoked prawn and fried prawn was: 87.85° 52.5° 55.94° and 53.98°. The tiger prawn processed by the smoking method has preferable by panelist rather than processed by boiling and frying.

  6. PENGARUH APLIKASI SUMBER C- KARBOHIDRAT (TEPUNG TAPIOKA DAN FERMENTASI PROBIOTIK PADA BUDIDAYA UDANG WINDU, Penaeus monodon POLA INTENSIF DI TAMBAK

    Directory of Open Access Journals (Sweden)

    Gunarto Gunarto

    2016-11-01

    Full Text Available Penelitian bertujuan untuk membandingkan pengaruh penambahan sumber C- karbohidrat (tepung tapioka dan fermentasi probiotik pada budidaya udang windu dengan pola intensif di tambak terutama melihat efeknya terhadap perbaikan kualitas air, pertumbuhan, sintasan, dan produksi udang windu. Enam petak tambak masing-masing ukuran sekitar 4.000 m2, setelah selesai tahap persiapan tambak (pengeringan, pembalikan tanah dasar, pengapuran, pengisian air, dan pemupukan, kemudian tambak ditebari tokolan udang windu PL-25 dengan padat tebar 20 ekor/m2. Tiga perlakuan diuji yaitu A. Penambahan tepung tapioka  ke air tambak dengan dosis 62% dari total pakan yang diberikan per hari dan diberikan dalam selang waktu lima hari sekali selama masa pemeliharaan pada bulan pertama dan kemudian dengan selang waktu tiga hari sekali selama masa pemeliharaan bulan kedua hingga menjelang panen; B. Pemberian fermentasi probiotik ke air tambak sebanyak 5 mg/L/minggu; dan C. Pemberian fermentasi probiotik ke air tambak sebanyak 10 mg/L/minggu. Masing-masing perlakuan dengan dua ulangan. Sampling pertumbuhan, kualitas air, dan bakteri dilakukan setiap dua minggu sekali. Sintasan, produksi, dan nilai konversi pakan dihitung setelah udang dipanen. Hasil penelitian menunjukkan bahwa pemberian tepung tapioka menyebabkan konsentrasi amoniak relatif lebih rendah di perlakuan A daripada di perlakuan B dan C, namun menunjukkan perbedaan yang tidak nyata (P>0,05 di antara ketiga perlakuan tersebut. Bahan Organik Total (BOT pada hari ke-112 di perlakuan C paling rendah dan menunjukkan perbedaan yang nyata (P0,05 di antara ketiga perlakuan yang diuji. The objective of the research was to compare the effect of addition of carbohydrate source (starch flour and probiotics fermentation to the water quality and the growth, survival, and production of tiger shrimp in intensive brackishwater pond system. Six pond compartments each sized approximately of 4,000 m2, went through preparation stages (pond drying, ploughing, liming, filling the pond with sea water and fertilyzing. Then the ponds were stocked with tiger shrimp post larvae day 25 at stocking density of 20 ind./m2. Three treatments were tested, A. the addition of starch flour in pond water column at a dosage of 62% of the total given feed per day, and applied every five days during the first month of shrimp culture, and then every three days from the second month to harvest time; B. the addition of probiotic fermentation to the pond water column and was given at 5 mg/L/week; and C. the addition of probiotic fermentation to the pond water column and was given at 10 mg/L/week. Result of the research showed that the addition of starch flour was able to decrease the ammonia concentration in treatment A, but there was no significant difference (P>0.05 with the ammonia concentration compared to the treatment B and C. Total Organic Matter (TOM at day 112 in treatment C was the lowest and significantly different (P0.05 among those treatments.

  7. Adherence And Pathogenicity Assay Of Vibrio Harveyi In Tiger Shrimp (Penaeus Monodon) Larvae For Screening Biocontrol Agent

    OpenAIRE

    SUWANTO, ANTONIUS; HALA, YUSMINAH; AFFANDI, RIDWAN; Jr., MUHAMMAD ZAIRIN

    2002-01-01

    Rifampicin-resistant marker was employed as a reporter to detect the adherence and colonization of V. harveyi in shrimp larvae. Vibrio harveyi P1B and YA32.2 were isolated from dead shrimp larvae in Besuki, Northern Coast of East Java, while V. harveyi HB3, was isolated from pristine sea water in Pacitan, Southern Coast of East Java. Vibrio metschnikovii used as biocontrol agent was isolated from healthy shrimp larvae in Serang, West Java. Spontaneous mutation was conducted to generate V. h...

  8. Strategy of Quality Improvement of Pond Shrimp Post Harvest Management (Penaeus monodon Fabricius in Mahakam Delta (Case Study

    Directory of Open Access Journals (Sweden)

    Andi Noor Asikin

    2012-10-01

    Full Text Available Mahakam Delta area, which is situated in Kutai Kartanegara Regency, has become a pond center that gives a significant contribution to shrimp export of East Kalimantan Province. Pond-produced shrimps, however, do not always accord to the intention of cold storage companies at expected price. The companies even sometimes reject the request due to poor quality of the product. The decreasing shrimp quality may be due to maintenance process by the pond farmers as well as the improper collectors. In the other hand, importing countries have decided more and more restricted requirements for the imported fishing products. This study was held in Muara Jawa, Anggana, and Muara Badak Districts using techniques of data collection of in-depth interview with twelve respondents. In order to improve shrimp quality, farmers or the producers have to formulate a strategy towards the improvement of the post harvest shrimps from the pond using an Analytical Hierarchy Process (AHP. Results of the AHP consist of as follows: (1 application of pond shrimp post harvest technology and improvement of facilities and accommodation that support the quality improvement of the pond shrimps, (2 human resource quality improvement of pond farmers, collectors, and field instructors, (3 development of pond culture by applying best practices principle and local policies in order to improve the quality of the pond shrimps, and (4 improvement of interagency coordination, monitoring, and evaluation to enhance the pond shrimp quality

  9. Effects of γ-irradiation and cooking on vitamins B6 and B12 in grass prawns (Penaeus monodon)

    International Nuclear Information System (INIS)

    Hau, L.-B.; Liew, M.-S.

    1993-01-01

    The effects of radiation doses, irradiation temperature and a combined treatment of irradiation and cooking on the vitamin B 6 and B 12 contents of grass prawns have been studied. Grass prawns were irradiated at refrigerated (4 o C) or frozen (-20 o C) temperatures with different doses. A domestic cooling procedure was followed after irradiation. The changes in vitamins B 6 and B 12 of both raw and cooked grass prawns were evaluated. Results showed no significant changes of vitamin B 6 and B 12 in grass prawns with a radiation dose up to 7 kGy at either 4 o C or -20 o C. Irradiation at 4 o C caused more destruction of vitamin B 12 but not vitamin B 6 than did irradiation at -20 o C in grass prawns. There was significant destruction of both vitamins B 6 and B 12 in unirradiated samples during cooking. The introduction of the irradiation process before cooking had no effect on either vitamin. These results indicate that the loss of vitamins B 6 and B 12 in the combined treatments was caused mainly by thermal destruction. (author)

  10. Hepatic and renal histology and mercury concentrations of North West and North East Greenland narwhals (Monodon monoceros)

    DEFF Research Database (Denmark)

    Sonne, Christian; Leifsson, Pall S.; Sondergaard, Jens

    2018-01-01

    subadult females, 8 adult females, 3 subadult males, and 1 adult male) and compare data with previous observations of 12 North West Greenland specimens (1 subadult female, 4 adult females, 1 subadult male, and 6 adult males). The zoological length was significantly larger and hepatic and renal...... of glomerular lesions (capillary dilatation [0–100%], membrane thickening [0–100%], dilatation of space [0–80%], and sclerosis [0–70%]) and two tubular lesions (atrophy/hyalinization [0–40%] and casts [0–60%]). Hepatic tissues exhibited three portal lesions (fibrosis [0–33%], cell infiltrates [0–40%], and bile...... duct proliferation [0–40%]) and two parenchymal lesions (steatosis [83–100%] and lipid-filled Ito cells [0–33%]). All 12 lesions were found in adult whales from North West Greenland while eight were present in adult whales from North East Greenland. Six lesions were detected in subadults from North...

  11. Pathogenesis and virulence of Vibrio harveyi from southern part of Thailand in black tiger shrimp, Penaeus monodon Fabricius

    Directory of Open Access Journals (Sweden)

    Pecharat, J.

    2004-01-01

    Full Text Available Ten isolates of bacteria were performed from diseased black tiger shrimp collected from farms in Nakhon Si Thammarat, Patthalung, Songkhla, Satun and Patthani. All isolates belonged to Vibrio harveyi, forming rounded colonies with smooth periphery and hemolysis of red blood cells. The colonies appearedgreen and yellow on TCBS selective media and indeeded both luminescent and non-luminescent groups. To support good growth as cell proliferation, an addition of 2-8% salt to the culture media was required. Sensitivity tests against antibiotics showed that all ten isolates were sensitive to chloramphenicol, norfloxacin, trimethoprim-sulfamethoxazole, oxolinic acid, oxytetracyclin and sarafloxacin at 80, 60, 50, 40, 40 and 30%, respectively. When 12-15 g juvenile shrimp were tested for the virulence by injection, the isolates were highly virulent with OD at 640 nm in the range 0.007-0.139. The bacteria concentration of 1.60×106 - 7.27×107 CFU/ ml caused 50% juvenile mortality in ten days. The physiological changes after susceptibility included a decline in the blood cells (p<0.05, higher plasma pH (p<0.05, decrease in serum protein within 24 h (p<0.05, and a return to normal levels within 48 h. There were no differences in blood sugar levels and phenoloxidase activity between the healthy and the infected individuals. Histological study showed that at an early period of susceptibility, there were swollen tubular lumen, minor cell degeneration of the hepatic tubules and lymphoid organs and aggregation of blood cells around the degenerating cells. During 7-day susceptibility period, there was large scale cell degeneration of the hepatopancreas, lymphoid organs, gills and blood formingorgans with a great extent of blood cell aggregation and eventually mortalities occurred. During 14 day susceptibility period, there was almost entire degeneration of cells in hepatic tubules and lymphoid organs causing hepatopancreatic tubular necrosis. During this period, juveniles did not accept feed and total mortality occurred.

  12. Inhibitory Mechanism of Robiotic Bacteria on The Growth of Vibrio harveyi in Tiger Shrimp (Penaeus monodon Larvae

    Directory of Open Access Journals (Sweden)

    . Widanarni

    2008-07-01

    Full Text Available Three probiotics named SKT-b, 1Ub, and Ua had inhibitory activity against the growth of Vibrio harveyi. These strains were mutated by rifampicin resistant. The inhibitory effect of SKT-b,1Ub, and Ua on the growth of V. harveyi was investigated by concomitant incubation of the two bacteria in a culture shrimp larvae. Colony forming unit of V. harveyi, probiotic and total of bacteria in dead, live larvae and water culture was monitored, and survival rate of larvae was investigated. Shrimp inoculated probiotic previously had survival rate higher than control (without probiotic. Number of V. harveyi in treatment without probiotic inoculation also higher compared to treatment with probiotic inoculation in dead, live larvae and water culture.  It demonstrated possible inhibition of probiotic bacteria on V. harveyi through competition for adherence sites or nutrition source. Partial sequencing of 16S-rRNA gene showed that 1Ub was similar to Pseudoalteromonas piscicida, whereas SKT-b and Ua were similar to Vibrio alginolyticus. Keywords: probiotic bacteria, inhibitory mechanism, V. harveyi, tiger shrimp   ABSTRAK Tiga isolat bakteri probiotik yaitu 1Ub, SKT-b dan Ua telah diuji memiliki aktivitas penghambatan terhadap pertumbuhan V. harveyi secara in vitro. Ketiga isolat ini kemudian diberi penanda resisten rifampisin (RfR melalui mutasi spontan untuk mempelajari mekanisme penghambatannya pada larva udang windu.  Efek penghambatan dari 1Ub, SKT-b dan Ua terhadap pertumbuhan V. harveyi diamati melalui pemberian secara bersamaan antara bakteri probiotik dan V. harveyi tersebut dalam air pemeliharaan larva udang.  Jumlah sel bakteri probiotik, V. harveyi dan total bakteri baik pada larva mati, larva hidup dan air pemeliharaan diamati dan kelangsungan hidup larva dihitung.  Nilai kelangsungan hidup udang pada perlakuan yang diinokulasi bakteri probiotik lebih tinggi daripada kontrol (tanpa penambahan bakteri probiotik. Jumlah sel V. harveyi pada perlakuan tanpa penambahan probiotik juga lebih tinggi, dibanding pada perlakuan dengan penambahan probiotik baik pada larva mati, larva hidup maupun air media pemeliharaan. Hal ini menunjukkan adanya penghambatan bakteri probiotik terhadap V. harveyi yang kemungkinan melalui kompetisi tempat pelekatan atau sumber nutrisi. Hasil analisis sekuen sebagian gen 16-rRNA menunjukkan bahwa isolat 1Ub termasuk spesies Pseudoalteromonas piscicida, sedangkan SKT-b dan Ua termasuk spesies Vibrio alginolyticus.  Kata kunci: bakteri probiotik, mekanisme penghambatan, V. harveyi, udang windu

  13. Localization of VP28 on the baculovirus envelope and its immunogenicity against white spot syndrome virus in Penaeus monodon

    International Nuclear Information System (INIS)

    Syed Musthaq, S.; Madhan, Selvaraj; Sahul Hameed, A.S.; Kwang, Jimmy

    2009-01-01

    White spot syndrome virus (WSSV) is a large dsDNA virus responsible for white spot disease in shrimp and other crustaceans. VP28 is one of the major envelope proteins of WSSV and plays a crucial role in viral infection. In an effort to develop a vaccine against WSSV, we have constructed a recombinant baculovirus with an immediate early promoter 1 which expresses VP28 at an early stage of infection in insect cells. Baculovirus expressed rVP28 was able to maintain its structural and antigenic conformity as indicated by immunofluorescence assay and western blot analysis. Interestingly, our results with confocal microscopy revealed that rVP28 was able to localize on the plasma membrane of insect cells infected with recombinant baculovirus. In addition, we demonstrated with transmission electron microscopy that baculovirus successfully acquired rVP28 from the insect cell membrane via the budding process. Using this baculovirus displaying VP28 as a vaccine against WSSV, we observed a significantly higher survival rate of 86.3% and 73.5% of WSSV-infected shrimp at 3 and 15 days post vaccination respectively. Quantitative real-time PCR also indicated that the WSSV viral load in vaccinated shrimp was significantly reduced at 7 days post challenge. Furthermore, our RT-PCR and immunohistochemistry results demonstrated that the recombinant baculovirus was able to express VP28 in vivo in shrimp tissues. This study will be of considerable significance in elucidating the morphogenesis of WSSV and will pave the way for new generation vaccines against WSSV.

  14. Horizontal transmission dynamics of White spot syndrome virus by cohabitation trials in juvenile Penaeus monodon and P. vannamei

    NARCIS (Netherlands)

    Ngo Xuan, T.; Verreth, J.A.J.; Vlak, J.M.; Jong, de M.C.M.

    2014-01-01

    White spot syndrome virus (WSSV), a rod-shaped double-stranded DNA virus, is an infectious agent causing fatal disease in shrimp farming around the globe. Within shrimp populations WSSV is transmitted very fast, however, the modes and dynamics of transmission of this virus are not well understood.

  15. Identification of Stressors that Affect White Spot Syndrome Virus (WSSV) Infection and Outbreak in Pond Cultured Penaeus monodon

    NARCIS (Netherlands)

    Tendencia Alapide, E.; Verreth, J.A.J.

    2011-01-01

    White spot syndrome virus (WSSV) has been a big problem to the worldwide shrimp industry. Exposure to stressors related to physicochemical water parameters affect WSSV infection but not all WSSV infections result in outbreaks. This paper describes a detailed monitoring of important physicochemical

  16. siRNA injection induces sequence-independent protection in Penaeus monodon against white spot syndrome virus

    NARCIS (Netherlands)

    Westenberg, M.; Heinhuis, B.; Zuidema, D.; Vlak, J.M.

    2005-01-01

    White spot syndrome virus (WSSV) is a major disease in crustaceans, particularly shrimp, due to the current intensity of aquaculture practices. Novel strategies including vaccination to control this virus would be highly desirable. However, invertebrates lack a true adaptive immune response system

  17. Regional and inter annual patterns of heavy metals, organochlorines and stable isotopes in narwhals (Monodon monoceros) from West Greenland

    DEFF Research Database (Denmark)

    Dietz, R.; Riget, F.; Hobson, K.A.

    2004-01-01

    Samples of 150 narwhals obtained in different years from two West Greenland areas, Avanersuaq and Uummannaq, were compared for concentrations of and regional differences in heavy metals and organochlorines and stable-carbon and nitrogen isotopes. Cadmium, Hg, and Se concentrations increased....../age composition of the data. PCB and DDT concentrations in West Greenland narwhals were half those found in East Greenland and Svalbard. Stable-carbon isotope ratios in muscle of 150 narwhals showed a decreasing trend in the first year when they gradually reduced their dependency on mother's milk, after which...... between stable isotope ratios and metal and OC concentrations....

  18. Reproductive performances of wild male tiger shrimp Penaeus monodon post-injection of oocyte developer without eyestalk ablation

    Directory of Open Access Journals (Sweden)

    Dony Prasetyo

    2017-07-01

    Full Text Available ABSTRACT The aim of this study was to evaluate the effect of oodev on the gonadal maturation and characteristic of spermatophore and spermatozoa produced by the wild male black tiger shrimp against eyestalk ablation. The treatments were two doses of oodev injection at 0.5 (OD0.5 and 1 (OD1.0 mL/kg of body weight and a control was eyestalk ablation (AB. The male stock of tiger shrimp used was from wild with body weight ranged from 55‒85 g, stocked into three of 10 tonnages concrete tanks with density of 25 males/tank. Oodev injection was applied for two times with one week interval. Tiger shrimp of OD0.5 group produced the highest number of gonadal maturing which was 84% followed by AB (68% and OD1.0 (64%. Oodev injection was able to shorten the time required for spermatophore maturation at maturation phase which happened simultaneously within 7 days post injection compared to that of ablated males. Positive correlation (R2=0.612 was detected between the shrimp weight and weight of spermatophore of the tiger shrimp while correlation between spermatophore weight and number of spermatozoa was relatively low (R2=0.415. Total fatty acid tented to be higher in males injected with oodev compared to ablated males. Concentration of arachidonic acid in the muscle of male stock in OD1.0 group was extremely low of 0.0037% of total lipid compared to AB (0.3190% and OD0.5 (0.2806%. Oodev injection at the dose of 0.5 mL/kg of tiger shrimp could improve the number of males stock producing spermatophore compared to eyestalk ablation. Simultaneously matured-spermatophore of wild male tiger shrimp within short time could be achieved through oodev injection. Keywords: oodev, spermatophore, reproduction, tiger shrimp  ABSTRAK Tujuan penelitian ini adalah mengevaluasi efek hormon oodev terhadap pematangan gonad dan karakter spermatofor dan spermatozoa yang dihasilkan oleh induk udang windu jantan alam dibandingkan dengan teknik ablasi tangkai mata. Perlakuan yang dicobakan adalah dua dosis injeksi oodev yaitu 0,5 (OD0,5 dan 1 (OD1.0 mL/kg bobot tubuh dan kontrol yaitu ablasi tangkai mata. Induk udang windu jantan yang digunakan berasal dari alam berbobot antara 55‒85 g, ditebar dalam tiga bak pematangan gonad berkapasitas 10 ton dengan kepadatan 25 ekor/bak. Injeksi oodev dilakukan dua kali dengan interval satu minggu. Induk udang windu pada OD0,5 menghasilkan jumlah induk matang gonad tertinggi yaitu 84%, diikuti oleh AB (68% dan OD1 (64%. Injeksi oodev mampu mempersingkat masa pematangan spermatofor pada fase maturasi dan pematangan tersebut terjadi secara simultan  setelah 7 hari pascainjeksi dibandingkan dengan induk yang di ablasi. Korelasi positif (R2=0,612 dideteksi antara bobot tubuh dan bobot spermatofor induk udang windu, sedangkan korelasi antara bobot spermatofor dan jumlah spermatozoa relatif lemah (R2=0,415. Total asam lemak daging cenderung lebih besar pada induk udang windu jantan yang diinjeksi oodev dibandingkan yang diablasi. Konsentrasi arachidonic acid dalam daging induk yang diinjeksi OD1 sangat rendah sebesar 0,0037% dari lemak  dibandingkan AB (0,3190% dan OD0,5 (0,2806%. Injeksi oodev pada dosis 0,5 mL/kg udang windu dapat meningkatkan jumlah induk yang menghasilkan spermatofor dibandingkan ablasi tangkai mata. Pematangan gonad udang windu jantan alam secara simultan dan singkat dapat dilakukan melalui injeksi oodev.   Kata kunci: oodev, spermatofor, reproduksi, udang windu

  19. INDUKSI PEMATANGAN GONAD DAN PENINGKATAN TINGKAT PEMBUAHAN TELUR INDUK UDANG WINDU, Penaeus monodon MELALUI RANGSANGAN HORMONAL TANPA ABLASI MATA

    Directory of Open Access Journals (Sweden)

    Asda Laining

    2015-03-01

    Full Text Available Penelitian ini bertujuan untuk mengetahui pengaruh rangsangan hormon gonadotropin (GTH dan antidopamin (AD terhadap performa induk betina alam sebagai pengganti ablasi. Dua perlakuan yang dicobakan adalah: 1 rangsangan hormon GTH dan AD pada betina udang windu alam tanpa ablasi; 2 kontrol berupa ablasi induk udang windu tanpa rangsangan hormon. Hasil yang diperoleh menunjukkan bahwa pemijahan pertama terjadi setelah rangsangan ke-2 yaitu 4 ekor induk (23,5%. Pascainjeksi ke-3, jumlah induk yang memijah meningkat menjadi 92,7% atau sebanyak 11 ekor dari 12 ekor yang diinjeksi. Setelah empat kali rangsangan, dari 17 ekor yang diinjeksi ternyata 14 ekor dapat memijah (82% dan 3 ekor induk tidak memijah hingga akhir pengamatan. Kontrol menghasilkan 6 ekor induk memijah dari 10 ekor yang diablasi (60%. Fekunditas telur (fertil dan tidak fertil dari induk yang dirangsang GTH+AD adalah 219.313 butir/induk lebih tinggi dibandingkan induk yang diablasi yaitu 182.848 butir/induk. Tingkat pembuahan pada kontrol adalah 54%, lebih rendah jika dibandingkan dengan jumlah telur yang fertil dari induk yang dirangsang GTH+AD yaitu 76%. Meskipun tingkat pembuahan telur yang dihasilkan oleh induk pascainjeksi lebih tinggi, ternyata tingkat penetasan telurnya hanya 39%, lebih rendah dari induk yang diablasi yaitu 52%. Diameter telur dari induk yang mendapat rangsangan AD+GTH adalah 29,3 μm ± 0,8 (rerata ± SD lebih besar dari diameter telur induk yang diablasi (25,1 μm ± 0,3. Total asam amino karkas induk udang yang dirangsang GTH+AD adalah 61,4% relatif lebih tinggi dibandingkan kontrol. Berdasarkan jumlah induk yang matang gonad dan fekunditas telur yang dihasilkan, aplikasi AD+GTH secara injeksi cukup dilakukan 3x pada dosis 0,3 mL/100 g udang.

  20. PROGRAM APRESIASI BAGI PEMERINTAH DAERAH (PEMDA DALAM MELAKSANAKAN TATA KELOLA (GOOD GOVERNANCE GUNA MEMAJUKAN KEPARIWISATAAN DI DAERAH

    Directory of Open Access Journals (Sweden)

    Ismayanti Ismayanti

    2015-09-01

    Full Text Available The success of region is largely determined by the commitment of the local government in the development of tourism. Good governance will certainly provide prosperity for the community. This first year multi-disciplinary research has a purpose: to design blueprint appreciation program for local governments that are committed in developing regions of Tourism through good governance and delivering award of appreciation to the government. This study examines several aspects of appreciation  through commitment identification and governance survey using analytical methods of AHP. Tourism appreciation blueprint is implemented with program testing. The results achieved is the publication and implementation of the blueprint of appreciation in cooperation with the Eljohn Publishing through Travel Club Tourism Award 2012 from the magazine Travel Club. The results is 40 nominees from the provincial governments, district and city governments. From the nominees, it is determined the winner of the provincial government consisting of 3 province, 9 city and 13 district government. Positive implications occurs after administration of appreciation and local prideness increases when each winner received awars and regional leaders gave speech in front of live audience and broadcasting live all over Indonesia. Besides these, dignity of each government showed in local and national publications in the electronic media and print media. Of these positive implications, it is recommended to conduct a similar program on a regular basis and measured the impact of the annual tribute to the development of tourism in the area so that the program becomes one measure the progress of regional tourismDOI: 10.15408/ess.v4i1.1962

  1. Perancangan Tata Kelola Teknologi Informasi di BAPAPSI Pemkab Bandung Menggunakan framework COBIT 5 Pada Domain EDM dan DSS

    Directory of Open Access Journals (Sweden)

    Rati Amanda Fajrin

    2016-10-01

    Full Text Available Information technology has an important role to support the implementation of public information. Bandung regency government has one of the bodies, named BAPAPSI. The existing problems in BAPAPSI, especially in PPI are uneven internet network in all districts, bad internet connection, bad handling of the incident, and the procurement of servers that do not support the use of the application in BAPAPSI. Therefore, 7 lifecycle COBIT 5 methodology is used to solve the problems in BAPAPSI. COBIT 5 provides complete, comprehensive guidelines in helping organizations to achieve the effective use of IT. The first stage in this research is assess capability level, then the next stage are mapping governance objective, enterprise goals, IT Related Goals and IT Process on COBIT 5. So, the results are the priority process on EDM04 and DSS01 domain to be analyzed and to design procedure IT governance using COBIT 5. This research also recommends the target of organizational structure in accordance with the needs of the Bandung regency government.

  2. Presence of radioactive minerals in the blast furnace slag of Tata steel and their bioaccumulation by some naturally growing plants

    Energy Technology Data Exchange (ETDEWEB)

    Singh, V K; Tej, P P [Dept. of Botany, Post Graduate Centre, Jamshedpur Co-Operative College, Jamshedpur (India)

    2005-07-01

    Twelve species of plants were sampled from the slag dumping area of Jamshedpur. Chemical analysis of soil as well as vegetation sample was carried out to detect the presence of {sup 238}U and {sup 226}Ra. The concentration of these two radionuclides was found to be elevated over the background sample. Commelina benghalensis, Parthenium hysterophorus, Andrographis paniculata, registered higher values of these radionuclides in their different organs. Such plants can be gainfully exploited for eco-restoration programme through phyto-remediation of the contaminated substratum. (author)

  3. Presence of radioactive minerals in the blast furnace slag of Tata steel and their bioaccumulation by some naturally growing plants

    International Nuclear Information System (INIS)

    Singh, V.K.; Tej, P.P.

    2005-01-01

    Twelve species of plants were sampled from the slag dumping area of Jamshedpur. Chemical analysis of soil as well as vegetation sample was carried out to detect the presence of 238 U and 226 Ra. The concentration of these two radionuclides was found to be elevated over the background sample. Commelina benghalensis, Parthenium hysterophorus, Andrographis paniculata, registered higher values of these radionuclides in their different organs. Such plants can be gainfully exploited for eco-restoration programme through phyto-remediation of the contaminated substratum. (author)

  4. ANALISIS TINGKAT KEMATANGAN PENYEDIAAN TATA KELOLA TEKNOLOGI INFORMASI DI PDII LIPI MENGGUNAKAN FRAMEWORK COBIT 4.1

    Directory of Open Access Journals (Sweden)

    Rima Octavia

    2014-12-01

    Full Text Available Centre for Scientific Documentation and Information – Indonesian Institute of Science (PDII LIPI began to developbusiness objectives towards a digital library. To support this goal should be held IT governance while PDII LIPI hasno specific IT governance. This research aims to study the condition of the IT management and determine the levelof maturity of the provision of IT governance framework based on COBIT 4.1. COBIT is one of the internationalstandard for IT governance. COBIT is used in this study were able to measure the COBIT 4.1 for organizations thatdo not use IT governance. Analysis of the level of maturity of the provision of IT governance is based on aquestionnaire that is processed by the COBIT 4.1 framework and compared with the actual conditions in the ITmanagement in PDII LIPI. The results of the calculation of the maturity level of to provided IT governance in PDIILIPI based on framework COBIT 4.1 shows that PDII-LIPI already at level 3 (Defined Process. This is consistent withthe actual conditions in PDII-LIPI. COBIT 4.1 is considered quite capable, reliable and easy to implement in PDII LIPI. 

  5. Modelling the mechanical behaviour of heterogeneous Ta/TA6V welded joints: behaviour and failure criteria

    International Nuclear Information System (INIS)

    Paris, Th.

    2008-12-01

    As laser welding of two different materials (heterogeneous welding) leads to a joint having a characteristic size close to the millimetre, i.e. much smaller than that of a structure, and as such a junction displays completely different mechanical properties because of the metallurgical transformations induced by intense thermal loading, the aim of this research thesis is to develop a behaviour model, flexible and robust enough, to represent all together the mechanical behaviours of the Ta, the TA6V and the melted zone. This model must be able to take plasticity and visco-plasticity into account, and also to provide a failure criterion through damage mechanics and its coupling with the behaviour. The author first reports the experimental characterization of the base materials (Ta and TA6V) by using tensile tests under different strain rates and different directions, relaxation tests and fatigue shear tests. He also characterizes the melted zone by describing the influence of a thermal treatment (induced by welding) on the formation of the melted zone, and by using different tests: four point bending on notched specimens, nano-indentation test, and longitudinal tensile test. In a second part, the author develops the model within the framework of continuum thermodynamics, and explores the numerical issues. The last part deals with the validation of the model for the concerned materials (Ta and TA6V) and melted zone

  6. A national competitiveness-based portfolio approach for international strategic management: illustrated with the case of the TATA industries

    NARCIS (Netherlands)

    Schiele, Holger; Harms, Rainer; Banerjee, Shrestho

    2014-01-01

    International firms with multiple business units are dealing with the question of how to balance the international dimension of their business portfolio. Which business units should go international and where should they contract or expand their international activities? Previous research lacks

  7. STRUKTUR KEPEMILIKAN, MEKANISME TATA KELOLA PERUSAHAAN, DAN BIAYA KEAGENAN DI INDONESIA (Studi Empirik pada Perusahaan di Bursa Efek Indonesia

    Directory of Open Access Journals (Sweden)

    Paulus Basuki Hadiprajitno

    2013-05-01

    Full Text Available This study is to examine the effect of corporate governance (the owners’s character and thegovernance mechanism on agency cost. This research shows that family ownership, financialinstitution ownership, government ownership, and foreign ownership have negative effects onagency cost that is proxied as managerial operation cost and or as assets turnover, compared topublic ownership. Furthermore, ownership concentration of at least 5 percent each is not provento effect the agency cost. Results of other examination show that the proportion of independentboard and the number of board meetings have negative effects to the agency cost that is proxiedas assets turnover. However, these two variables and the number of audit committee meetinghave the opposite effect to the agency cost when it is proxied as managerial operation cost.Theseinconsistencies are caused by the ineffective implementation of corporate governance mechanismin Indonesia even after 10 years of implementation.

  8. PENERAPAN SISTEM PENGENDALIAN INTERN UNTUK MENGANTISIPASI PRAKTEK-PRAKTEK YANG TIDAK SEHAT DALAM TATA KELOLA KEUANGAN KOPERASI DI KABUPATEN GIANYAR

    Directory of Open Access Journals (Sweden)

    I D. N. Wiratmaja

    2012-10-01

    Full Text Available Social function inherent in the cooperative as a business entities and weak internal control is the trigger high levels of fraud in the cooperatives. Fraud carried out by unscrupulous managers is the main cause of the high level of bankruptcies in the cooperative. As the concern over the condition academic community of Udayana University through community service agency LPM organizes a lecture to introduce internal control for cooperation in Gianyar Regency. Lecturing was carried out on Saturday 27 September 2009 in Tampaksiring involving 56 participants from 19 cooperations throughout the Gianyar Regency. The evaluation result showed that the response of participants was positive and hoped that similar activities can be conducted on an ongoing basis. The main topics proposed by participants to the sustainability of the program is, training on the preparation of financial statements, training of computerized transaction processing, entrepreneurship and taxation.

  9. Perancangan Tata Kelola Layanan Teknologi Informasi Menggunakan ITIL versi 3 Domain Service Transition Dan Service Operation Di Pemerintah Kota Bandung

    Directory of Open Access Journals (Sweden)

    Luki Aisha Kusuma Wardani

    2016-10-01

    Full Text Available Nowadays, Information Technology plays an important role in an organization, and not only as a support equipment. Bandung City Government, as a government agency, make IT into one of the visions that need to be achieved. To achieve good governance based on Minister Regulation Number 41 in 2007, IT Governance is needed to ensure that the investment of IT can add value and align with institution’s objective. Bandung City Government currently doesn’t have policies and procedure that regulate about IT Service Management. Therefore, this study was conducted with the aim to create a guidance for Office of Communications and Information Technology of Bandung (Diskominfo to optimize the service provided. This study also aims to make Service Management governance so that the management of IT service have certain quality and meet the needs of business customers, and improve the quality as the agreement with the customers. This study was conducted using ITIL v3 with Service Operation and Service Transition domain. ITIL is IT Service Management best practice to improve the efficiency of IT operational. ISO 15504 was conducted to asses Capability Level of ITIL Process. Capability Level of Service Transition is 24.5% and Capability Level of Service Operation is 23%. Because of the inadequacy of the primary data, the assessment was also carried out by mapping the objective of institution with the risks related to the lack of ITIL processes. The results of the design are the governance policies and procedures related to the ITIL processes needed by the institution.

  10. Analisa Ketelitian Orthorektifikasi Citra Pleiades untuk Pembuatan Peta Rencana Detail Tata Ruang Terbuka Hijau (Studi Kasus: Kota Surabaya

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    Meika Sumarsono

    2017-01-01

    Full Text Available Surabaya merupakan salah satu kota besar yang mempunyai banyak penduduk sehingga perlu adanya pengembangan RTH yang cukup dalam suatu wilayah agar kondisi lingkungan tetap nyaman salah satunya dengan pembuatan peta RDTR RTH menggunakan citra satelit resolusi tinggi. Namun citra satelit resolusi tinggi perlu dikoreksi secara geometrik dengan orthorektifikasi untuk memposisikan kembali citra sesuai lokasi sebenarnya karena pada saat peliputan data terjadi pergeseran posisi. Penelitian ini mengkaji ketelitian orthorektifikasi citra Pleiades 1A dengan menggunakan metode RPC sebagai peta dasar untuk rekomendasi dalam pembuatan peta RDTR RTH di wilayah Surabaya bagian pusat. Data yang digunakan dalam penelitian ini yaitu citra Pleiades 1A dengan resolusi spasial 0,5 m pankromatik dan 2 m multispektral, DEM ALOS PALSAR dengan ketelitian 12,5 m, Ground Control Point (GCP hasil dari pengukuran di lapangan. Ketelitian geometrik menggunakan metode RPC di mana metode ini menggunakan informasi parameter orientasi dalam yang terdapat di metadata serta dibantu dengan titik kontrol tanah dan DEM. Hasil proses orthorektifikasi dengan menggunakan 8 titik GCP berupa RMSE sebesar 0,36 piksel dan 0,18 m yang memenuhi syarat untuk pembuatan peta dasar skala 1:5000. Hasil orthorektifikasi ini diuji dengan cara tumpang tindih (overlay terhadap peta skala 1:25.000.

  11. PENGARUH KOMUNIKASI NON FORMAL, DISIPLIN KERJA, KOMPETENSI PEGAWAI DAN EMPATI PEGAWAI TATA USAHA TERHADAP PELAYANAN SISWA SMK PALEBON SEMARANG

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    Ary Fidayatul Ikhsani

    2017-02-01

    Full Text Available Services students` is one important component in supporting the learning process. If the service provided is poor then the students will find it difficult to take care of all administrative and obtain the information needed. Based on data from interviews with five vocational students obtained Palebon Semarang is still a gap between expectations and the reality of the service given to vocational students Administrative Palebon Semarang. Based on the background of the above problems can be obtained formulation of the problem is there any influence of non-formal communication, work discipline, employee competence and empathy clerical employees with the services of vocational students Palebon Semarang ?. This study aims to determine whether there is influence of non-formal communication, work discipline, employee competence and empathy towards employees Administrative Services Vocational students Palebon Semarang. , This study includes quantitative research. Methods of data collection using the questionnaire. Methods of data analysis using multiple linear regression, the classic assumption test, test hypotheses and descriptive analysis. Y = 1.775 + 0.277 + 0.123 X1 + 0.285 X2 X3 + X4 + 0.39 e. The test results obtained Fhitung 57 601 simultaneous with significance 0.000 <0.05 .. The amount of influence simultaneously the influence of non-formal communication, work discipline, employee competence and empathy employees with the services of 61.3%. Influence in partial non formal communication that is 12.5%, which is 3.8% of labor discipline, the competence of employees is 13.2%, while empathy is 16.6%

  12. Efektivitas Kelembagaan Pemerintah dalam Integrasi Pola Pengelolaan Sumber Daya Air dalam Rencana Tata Ruang Wilayah (Studi Kasus: Provinsi Jawa Tengah

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    Silviani Junita

    2016-04-01

    Full Text Available The effectiveness of integrating water resources management into spatial planning (RTRW can be achieved if there is a harmonious coordination between central government and local government. This research aims to analyze the role of and coordinating pattern of inter-sectoral institutions related to water resource planning and management in spatial planning. The research is conducted by using qualitative approach through the analysis of the role of and coordination in inter-sectoral institutions and the effectiveness of the successful integration of water resources management in spatial planning. The findings show that the integration of water resources management pattern in the spatial planning is ineffective indicated by the ineffectiveness of government institution as the policy maker and the lack of coordination between institutions regarding the content of water resource management in spatial planning. Therefore, it is necessary to make a coordination forum to accommodate the interests of water resources and spatial planning.

  13. PEMBERDAYAAN EKONOMI UMMAT MELALUI ZAKAT PRODUKTIF (Studi Kritik Atas Tata Kelola Badan Amil Zakat Nasional [BAZNAS] Kabupaten Nganjuk

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    Mukhamat Saini

    2016-09-01

    Full Text Available The concept of zakat economy performed in Nganjuk, has a fairly wide spectrum. It is not just the caricature and religious rituals, but more empowerment. Zakat is not only as a form of worship to God or moral obligation for Muslims, but also has function as fiscal alternative policy instruments to achieve the equal distribution of income among Muslims. Provision of social assistance from the rich to the poor or to other mustahiq. Zakat is expected to uplift the poor and helping the way out of the difficulties of life. Helping the problems solving faced by mustahiq, get rid of niggardly nature and tighten the brotherhood among Muslims. Normatively, Zakat practiced in the lives of people in Nganjuk is the worship of socio-economics patterned, which can be used to prevent the accumulation of wealth in the minority of people and narrow the economic gaps in society. On the other hand, it also becomes a kind of social security act for "the loser" in the development process, and as social safety nets (certainty of the fulfillment of minimum rights the poor that has as a control against the human nature which tends to be happy to the wealth accumulation. We can collect data on the level of the real needs of mustahiq zakat groups, zakat funds capabilities, and mustahik conditions thus leading to increase in the human welfare in Nganjuk. Empowerment in this context implies giving zakat to mustahiq productively with the aim that zakat will bring results and benefits. The distribution of zakat seen from its form can be done in two ways, namely: First, the instantaneous form and mustahiq zakat empowerment form productively, mustahiq in this category is mustahik of eight ashnaf that have ability, potential and power to work. Second, the distribution of empowerment form is the distribution of zakat which accompanied the target of changing mustahiq conditions into muzakki categories.

  14. Targeting TBP-associated factors in ovarian cancer

    Directory of Open Access Journals (Sweden)

    Jennifer R Ribeiro

    2014-03-01

    Full Text Available As ovarian tumors progress, they undergo a process of dedifferentiation, allowing adaptive changes in growth and morphology that promote metastasis and chemoresistance. Herein, we outline a hypothesis that TATA-box binding protein (TBP associated factors (TAFs, which compose the RNA Polymerase II initiation factor, TFIID, contribute to regulation of dedifferentiation states in ovarian cancer. Numerous studies demonstrate that TAFs regulate differentiation and proliferation states; their expression is typically high in pluripotent cells and reduced upon differentiation. Strikingly, TAF2 exhibits copy number increases or mRNA overexpression in 73% of high grade serous ovarian cancers (HGSC. At the biochemical level, TAF2 directs TFIID to TATA-less promoters by contact with an Initiator element, which may lead to the deregulation of the transcriptional output of these tumor cells. TAF4, which is altered in 66% of HGSC, is crucial for the stability of the TFIID complex and helps drive dedifferentiation of mouse embryonic fibroblasts to induced pluripotent stem cells. Its ovary-enriched paralog, TAF4B, is altered in 26% of HGSC. Here, we show that Taf4b mRNA correlates with Cyclin D2 mRNA expression in human granulosa cell tumors. TAF4B may also contribute to regulation of tumor microenvironment due to its estrogen-responsiveness and ability to act as a cofactor for NFκB. Conversely, TAF9, a cofactor for p53 in regulating apoptosis, may act as a tumor suppressor in ovarian cancer, since it is downregulated or deleted in 98% of HGSC. We conclude that a greater understanding of mechanisms of transcriptional regulation that execute signals from oncogenic signaling cascades is needed in order to expand our understanding of the etiology and progression of ovarian cancer, and most importantly to identify novel targets for therapeutic intervention.

  15. TAF(II)250: a transcription toolbox.

    Science.gov (United States)

    Wassarman, D A; Sauer, F

    2001-08-01

    Activation of RNA-polymerase-II-dependent transcription involves conversion of signals provided by gene-specific activator proteins into the synthesis of messenger RNA. This conversion requires dynamic structural changes in chromatin and assembly of general transcription factors (GTFs) and RNA polymerase II at core promoter sequence elements surrounding the transcription start site of genes. One hallmark of transcriptional activation is the interaction of DNA-bound activators with coactivators such as the TATA-box binding protein (TBP)-associated factors (TAF(II)s) within the GTF TFIID. TAF(II)250 possesses a variety of activities that are likely to contribute to the initial steps of RNA polymerase II transcription. TAF(II)250 is a scaffold for assembly of other TAF(II)s and TBP into TFIID, TAF(II)250 binds activators to recruit TFIID to particular promoters, TAF(II)250 regulates binding of TBP to DNA, TAF(II)250 binds core promoter initiator elements, TAF(II)250 binds acetylated lysine residues in core histones, and TAF(II)250 possesses protein kinase, ubiquitin-activating/conjugating and acetylase activities that modify histones and GTFs. We speculate that these activities achieve two goals--(1) they aid in positioning and stabilizing TFIID at particular promoters, and (2) they alter chromatin structure at the promoter to allow assembly of GTFs--and we propose a model for how TAF(II)250 converts activation signals into active transcription.

  16. Non-canonical TAF complexes regulate active promoters in human embryonic stem cells.

    Science.gov (United States)

    Maston, Glenn A; Zhu, Lihua Julie; Chamberlain, Lynn; Lin, Ling; Fang, Minggang; Green, Michael R

    2012-11-13

    The general transcription factor TFIID comprises the TATA-box-binding protein (TBP) and approximately 14 TBP-associated factors (TAFs). Here we find, unexpectedly, that undifferentiated human embryonic stem cells (hESCs) contain only six TAFs (TAFs 2, 3, 5, 6, 7 and 11), whereas following differentiation all TAFs are expressed. Directed and global chromatin immunoprecipitation analyses reveal an unprecedented promoter occupancy pattern: most active genes are bound by only TAFs 3 and 5 along with TBP, whereas the remaining active genes are bound by TBP and all six hESC TAFs. Consistent with these results, hESCs contain a previously undescribed complex comprising TAFs 2, 6, 7, 11 and TBP. Altering the composition of hESC TAFs, either by depleting TAFs that are present or ectopically expressing TAFs that are absent, results in misregulated expression of pluripotency genes and induction of differentiation. Thus, the selective expression and use of TAFs underlies the ability of hESCs to self-renew.DOI:http://dx.doi.org/10.7554/eLife.00068.001.

  17. Identification of a domain within human TAF(I)48, a subunit of Selectivity Factor 1, that interacts with helix 2 of TBP.

    Science.gov (United States)

    Xu, Shuping; Hori, Roderick T

    2004-09-01

    RNA polymerase I transcription in human cells requires Selectivity Factor 1, a multisubunit complex composed of the TATA-box-binding protein (TBP) and three TBP-associated factors (TAFs) called TAF(I)48, TAF(I)63 and TAF(I)110. Each of the Selectivity Factor 1 subunits binds directly to the other three components, but these interactions have not been characterized. This study is the initial identification and analysis of a TBP-binding domain within a Selectivity Factor 1 TAF. The interaction between human TBP and human TAF(I)48 was initially examined using the yeast two-hybrid assay, and a TBP-binding domain was identified in the carboxyl-terminus of human (h)TAF(I)48. Consistent with this result, the hTAF(I)48 carboxyl-terminus was able to bind directly to TBP in protein-protein interaction assays. When mutations were introduced into the hTAF(I)48 carboxyl-terminus, we identified changes in uncharged and positive residues that affect its interaction with TBP. By examining TBP mutants, residues within and adjacent to helix 2 of TBP, previously demonstrated to interact with subunits of other TBP-containing complexes [Transcription Factor IID (TFIID) and TFIIIB] were also found to diminish its affinity for the carboxyl-terminus of hTAF(I)48. The regions of hTAF(I)48 and TBP that interact are compared to those identified within other complexes containing TBP.

  18. Drosophila TRF2 and TAF9 regulate lipid droplet size and phospholipid fatty acid composition.

    Science.gov (United States)

    Fan, Wei; Lam, Sin Man; Xin, Jingxue; Yang, Xiao; Liu, Zhonghua; Liu, Yuan; Wang, Yong; Shui, Guanghou; Huang, Xun

    2017-03-01

    The general transcription factor TBP (TATA-box binding protein) and its associated factors (TAFs) together form the TFIID complex, which directs transcription initiation. Through RNAi and mutant analysis, we identified a specific TBP family protein, TRF2, and a set of TAFs that regulate lipid droplet (LD) size in the Drosophila larval fat body. Among the three Drosophila TBP genes, trf2, tbp and trf1, only loss of function of trf2 results in increased LD size. Moreover, TRF2 and TAF9 regulate fatty acid composition of several classes of phospholipids. Through RNA profiling, we found that TRF2 and TAF9 affects the transcription of a common set of genes, including peroxisomal fatty acid β-oxidation-related genes that affect phospholipid fatty acid composition. We also found that knockdown of several TRF2 and TAF9 target genes results in large LDs, a phenotype which is similar to that of trf2 mutants. Together, these findings provide new insights into the specific role of the general transcription machinery in lipid homeostasis.

  19. Identification of appropriate reference genes for human mesenchymal stem cell analysis by quantitative real-time PCR.

    Science.gov (United States)

    Li, Xiuying; Yang, Qiwei; Bai, Jinping; Xuan, Yali; Wang, Yimin

    2015-01-01

    Normalization to a reference gene is the method of choice for quantitative reverse transcription-PCR (RT-qPCR) analysis. The stability of reference genes is critical for accurate experimental results and conclusions. We have evaluated the expression stability of eight commonly used reference genes found in four different human mesenchymal stem cells (MSC). Using geNorm, NormFinder and BestKeeper algorithms, we show that beta-2-microglobulin and peptidyl-prolylisomerase A were the optimal reference genes for normalizing RT-qPCR data obtained from MSC, whereas the TATA box binding protein was not suitable due to its extensive variability in expression. Our findings emphasize the significance of validating reference genes for qPCR analyses. We offer a short list of reference genes to use for normalization and recommend some commercially-available software programs as a rapid approach to validate reference genes. We also demonstrate that the two reference genes, β-actin and glyceraldehyde-3-phosphate dehydrogenase, are frequently used are not always successful in many cases.

  20. Identification of stable reference genes for quantitative PCR in cells derived from chicken lymphoid organs.

    Science.gov (United States)

    Borowska, D; Rothwell, L; Bailey, R A; Watson, K; Kaiser, P

    2016-02-01

    Quantitative polymerase chain reaction (qPCR) is a powerful technique for quantification of gene expression, especially genes involved in immune responses. Although qPCR is a very efficient and sensitive tool, variations in the enzymatic efficiency, quality of RNA and the presence of inhibitors can lead to errors. Therefore, qPCR needs to be normalised to obtain reliable results and allow comparison. The most common approach is to use reference genes as internal controls in qPCR analyses. In this study, expression of seven genes, including β-actin (ACTB), β-2-microglobulin (B2M), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), β-glucuronidase (GUSB), TATA box binding protein (TBP), α-tubulin (TUBAT) and 28S ribosomal RNA (r28S), was determined in cells isolated from chicken lymphoid tissues and stimulated with three different mitogens. The stability of the genes was measured using geNorm, NormFinder and BestKeeper software. The results from both geNorm and NormFinder were that the three most stably expressed genes in this panel were TBP, GAPDH and r28S. BestKeeper did not generate clear answers because of the highly heterogeneous sample set. Based on these data we will include TBP in future qPCR normalisation. The study shows the importance of appropriate reference gene normalisation in other tissues before qPCR analysis. Copyright © 2016 Elsevier B.V. All rights reserved.

  1. Transcription initiation complex structures elucidate DNA opening.

    Science.gov (United States)

    Plaschka, C; Hantsche, M; Dienemann, C; Burzinski, C; Plitzko, J; Cramer, P

    2016-05-19

    Transcription of eukaryotic protein-coding genes begins with assembly of the RNA polymerase (Pol) II initiation complex and promoter DNA opening. Here we report cryo-electron microscopy (cryo-EM) structures of yeast initiation complexes containing closed and open DNA at resolutions of 8.8 Å and 3.6 Å, respectively. DNA is positioned and retained over the Pol II cleft by a network of interactions between the TATA-box-binding protein TBP and transcription factors TFIIA, TFIIB, TFIIE, and TFIIF. DNA opening occurs around the tip of the Pol II clamp and the TFIIE 'extended winged helix' domain, and can occur in the absence of TFIIH. Loading of the DNA template strand into the active centre may be facilitated by movements of obstructing protein elements triggered by allosteric binding of the TFIIE 'E-ribbon' domain. The results suggest a unified model for transcription initiation with a key event, the trapping of open promoter DNA by extended protein-protein and protein-DNA contacts.

  2. [Divergence of paralogous growth-hormone-encoding genes and their promoters in Salmonidae].

    Science.gov (United States)

    Kamenskaya, D N; Pankova, M V; Atopkin, D M; Brykov, V A

    2017-01-01

    In many fish species, including salmonids, the growth-hormone is encoded by two duplicated paralogous genes, gh1 and gh2. Both genes were already in place at the time of divergence of species in this group. A comparison of the entire sequence of these genes of salmonids has shown that their conserved regions are associated with exons, while their most variable regions correspond to introns. Introns C and D include putative regulatory elements (sites Pit-1, CRE, and ERE), that are also conserved. In chars, the degree of polymorphism of gh2 gene is 2-3 times as large as that in gh1 gene. However, a comparison across all Salmonidae species would not extent this observation to other species. In both these chars' genes, the promoters are conserved mainly because they correspond to putative regulatory sequences (TATA box, binding sites for the pituitary transcription factor Pit-1 (F1-F4), CRE, GRE and RAR/RXR elements). The promoter of gh2 gene has a greater degree of polymorphism compared with gh1 gene promoter in all investigated species of salmonids. The observed differences in the rates of accumulation of changes in growth hormone encoding paralogs could be explained by differences in the intensity of selection.

  3. Large Polyglutamine Repeats Cause Muscle Degeneration in SCA17 Mice

    Directory of Open Access Journals (Sweden)

    Shanshan Huang

    2015-10-01

    Full Text Available In polyglutamine (polyQ diseases, large polyQ repeats cause juvenile cases with different symptoms than those of adult-onset patients, who carry smaller expanded polyQ repeats. The mechanisms behind the differential pathology mediated by different polyQ repeat lengths remain unknown. By studying knockin mouse models of spinal cerebellar ataxia-17 (SCA17, we found that a large polyQ (105 glutamines in the TATA-box-binding protein (TBP preferentially causes muscle degeneration and reduces the expression of muscle-specific genes. Direct expression of TBP with different polyQ repeats in mouse muscle revealed that muscle degeneration is mediated only by the large polyQ repeats. Different polyQ repeats differentially alter TBP’s interaction with neuronal and muscle-specific transcription factors. As a result, the large polyQ repeat decreases the association of MyoD with TBP and DNA promoters. Our findings suggest that specific alterations in protein interactions by large polyQ repeats may account for the unique pathology in juvenile polyQ diseases.

  4. Large Polyglutamine Repeats Cause Muscle Degeneration in SCA17 Mice

    Science.gov (United States)

    Huang, Shanshan; Yang, Su; Guo, Jifeng; Yan, Sen; Gaertig, Marta A.; Li, Shihua; Li, Xiao-Jiang

    2015-01-01

    SUMMARY In polyglutamine (polyQ) diseases, large polyQ repeats cause juvenile cases with different symptoms than adult-onset patients, who carry smaller expanded polyQ repeats. The mechanisms behind the differential pathology mediated by different polyQ repeat lengths remain unknown. By studying knock-in mouse models of spinal cerebellar ataxia-17 (SCA17), we found that a large polyQ (105 glutamines) in the TATA box-binding protein (TBP) preferentially causes muscle degeneration and reduces the expression of muscle-specific genes. Direct expression of TBP with different polyQ repeats in mouse muscle revealed that muscle degeneration is mediated only by the large polyQ repeats. Different polyQ repeats differentially alter TBP’s interaction with neuronal and muscle-specific transcription factors. As a result, the large polyQ repeat decreases the association of MyoD with TBP and DNA promoters. Our findings suggest that specific alterations in protein interactions by large polyQ repeats may account for the unique pathology in juvenile polyQ diseases. PMID:26387956

  5. Predator-induced defences in Daphnia pulex: Selection and evaluation of internal reference genes for gene expression studies with real-time PCR

    Directory of Open Access Journals (Sweden)

    Gilbert Don

    2010-06-01

    Full Text Available Abstract Background The planktonic microcrustacean Daphnia pulex is among the best-studied animals in ecological, toxicological and evolutionary research. One aspect that has sustained interest in the study system is the ability of D. pulex to develop inducible defence structures when exposed to predators, such as the phantom midge larvae Chaoborus. The available draft genome sequence for D. pulex is accelerating research to identify genes that confer plastic phenotypes that are regularly cued by environmental stimuli. Yet for quantifying gene expression levels, no experimentally validated set of internal control genes exists for the accurate normalization of qRT-PCR data. Results In this study, we tested six candidate reference genes for normalizing transcription levels of D. pulex genes; alpha tubulin (aTub, glyceraldehyde-3-phosphate dehydrogenase (GAPDH, TATA box binding protein (Tbp syntaxin 16 (Stx16, X-box binding protein 1 (Xbp1 and CAPON, a protein associated with the neuronal nitric oxide synthase, were selected on the basis of an earlier study and from microarray studies. One additional gene, a matrix metalloproteinase (MMP, was tested to validate its transcriptional response to Chaoborus, which was earlier observed in a microarray study. The transcription profiles of these seven genes were assessed by qRT-PCR from RNA of juvenile D. pulex that showed induced defences in comparison to untreated control animals. We tested the individual suitability of genes for expression normalization using the programs geNorm, NormFinder and BestKeeper. Intriguingly, Xbp1, Tbp, CAPON and Stx16 were selected as ideal reference genes. Analyses on the relative expression level using the software REST showed that both classical housekeeping candidate genes (aTub and GAPDH were significantly downregulated, whereas the MMP gene was shown to be significantly upregulated, as predicted. aTub is a particularly ill suited reference gene because five copies are

  6. Effect of different mangrove-to-pond area ratios on influent water quality and WSSV occurence in panaeus monodon Semi-intensive farms using the greenwater culture technique

    NARCIS (Netherlands)

    Tendencia, E.A.; Bosma, R.H.; Primavera, J.H.; Verreth, J.A.J.

    2012-01-01

    White spot syndrome virus (WSSV) has been affecting the shrimp industry worldwide for two decades now. It continues to bring economic losses to affected farms. Despite the many studies on its epidemiology, there is no proven treatment or control measure. Diseases, like the WSSV, results from the

  7. Effects of Thai medicinal plants on pathogenic bacterial, growth performance, health condition and disease resistance in black tiger shrimp (Penaeus monodon Fabricius

    Directory of Open Access Journals (Sweden)

    Klowkliang, T.

    2005-02-01

    Full Text Available Chemical analysis of turmeric (Curcuma longa extracts using TLC/densitometry, showed an extract contain 21.57%w/w of three important curcuminoids: curcumin, desmethoxycurcumin and bisdesmethoxycurcumin. GC and MS were used to analyze volatile oils. Aromatic turmerone, α-turmerone and zingiberene were also obtained. Qualitative and quantitative analyses alcoholic extract of Andrographis paniculata using TLC, revealed that the extracts contain three important compounds in total lactone of 30.49% w/w. There are andrographolide,14-deoxy-11-12-didehydroandrographolide and neoandrographolide. TLC-chromatogram of Clinacanthus nutans extract after reacted with anisaldehyde/sulfuric acid showed a 9 key compounds, while preliminary neutralization test of the compounds revealed that there were active compounds against HSV-1 virus. In vitro efficacy test revealed that Curcuma longa and Andrographis paniculata extracts at 250 and 1,500 mg/L could eradicate 15 isolates of Vibrio spp. which were isolated from infected shrimps. Effects of medicinal plant extracts incorporated into the diet on shrimp immune responses were investigated. Shrimp fed diet containing Clinacanthus nutans extract at 20 mg/kg of diet had good growth, FCR and immune responses. The shrimp that were fed diet containing Curcuma longa extracts at 25 mg/kg of diet for 7-14 days showed high resistance to Vibrio harveyi. Likewise, the shrimp fed Andrographis paniculata extract at 25 mg/ kg of diet for 14 days had a higher resistance to WSSV. Incorporating the medicinal extracts at higher levels resulted in reduction in diet palatability which consequently had an effect on a decrease in growth, immune responses and resistance to bacterial and WSSV infection.

  8. Effect of three innovative culture systems on water quality and whitespot syndrome virus (WSSV) viral load in WSSV-fed Penaeus monodon cultured in indoor tanks

    NARCIS (Netherlands)

    Alapide-Tendencia, E.V.; Bosma, R.H.; Rose Sorio, L.

    2012-01-01

    White spot syndrome virus is the most important among the shrimp diseases. It has been devastating the shrimp industry for more than 3 decades. Previous studies reported that greater percentage of yellow colonies on thiosulfate citrate bile salt sucrose agar (yellow vibrios) in the rearing water,

  9. PERBAIKAN KUALITAS PETIS KEPALA UDANG WINDU (Penaeus monodon DENGAN PENAMBAHAN TEPUNG ARANG KAYU GALAM (Melaleuca cajuputi powell, SEKAM PADI (Oryza sativa L DAN TEMPURUNG KELAPA (Cocos nucifera

    Directory of Open Access Journals (Sweden)

    Juhana Suhanda

    2016-06-01

    Full Text Available Penelitian ini dilaksanakan dengan waktu selama 4 bulan yaitu meliputi  pelaksanaan penelitian, analisis data, penyusunan laporan. Penelitian ini bertujuan untuk membuat petis sari kepala udang Windu yang berkualitas dengan penambahan tepung arang kayu galam, sekam padi, dan tempurung kelapa. Sedangkan kegunaannya adalah untuk menghasilkan petis sari kepala udang Windu yang dapat diterima dan disukai oleh konsumen. Penelitian ini menggunakan metode eksperimental dan dilakukan berdasarkan pola Rancangan Acak Lengkap (RAL dengan empat perlakuan yaitu A (tanpa penambahan tepung arang, perlakuan B (penambahan tepung arang kayu Galam 0,05%, perlakuan C (penambahan tepung arang sekam padi 0,05% dan perlakuan D (penambahan tepung arang tempurung kelapa 0,05%. Masing-masing perlakuan dilakukan 3 kali ulangan. Parameter yang diuji meliputi Uji Kadar Protein, Uji Kadar Air, Uji Kadar Abu, Uji Organoleptik yaitu rasa, warna, aroma dan tekstur. Penerimaan atau penolakan terhadap hipotesis didasarkan pada hasil Uji F (Analisis Sidik Ragam Dari hasil pengujian dan analisis keempat perlakuan tersebut untuk uji kadar protein yang tertinggi adalah perlakuan D (26,60%, uji kadar air terendah adalah pada perlakuan D (19,28% dan uji kadar abu terendah adalah perlakuan A (7,77%, tetapi antar perlakuan pada kadar abu tidak berbeda nyata sehingga dapat diartikan kadar abu pada tiap perlakuan tidak berbeda (tidak signifikan, sedangkan uji organoleptik untuk rasa, warna, aroma dan tekstur yang tertinggi adalah perlakuan D yaitu rasa (6,89, warna (7,31, aroma (6,48 dan tekstur (6,635. This research was conducted with time for 4 months which include the conduct of research, data analysis, report preparation. This study aims to make a paste shrimp head juice Windu quality with the addition of flour Galam wood charcoal, rice husk and coconut shell. While its use is to produce a paste shrimp head juice Windu acceptable and preferred by consumers. This study used an experimental method based on the pattern and made completely randomized design ( CRD with four treatments, namely A ( without the addition of charcoal powder , treatment B ( addition of charcoal powder Galam 0.05 %, treatment C ( addition of rice husk flour 0 , 05 % and treatment D ( addition of coconut shell charcoal powder 0.05 % . Each treatment was done 3 times repetition. The parameters tested include Protein Levels Test, Test Water Content, Content Test Abu, Organoleptic Test is taste, color, aroma and texture. Acceptance or rejection of the hypothesis based on the results of the F test ( analysis of variance From the results of the testing and analysis of the four treatments for the highest levels of the protein test is treatment D ( 26.60 % , the lowest moisture content test is in treatment D ( 19 , 28 % and lowest ash content test is treatment A (7.77 % , but among the treatments on ash content was not significantly different so that could mean ash content in each treatment did not differ    ( not significant , whereas organoleptic test for taste , color , flavor  and texture which is the highest treatment D is a sense of taste ( 6.89 , color (7.31 , flavor (6.48 and texture (6.635.

  10. Evaluation of an immunodot test to manage white spot syndrome virus (WSSV) during cultivation of the giant tiger shrimp Penaeus monodon

    Digital Repository Service at National Institute of Oceanography (India)

    Patil, R.; Palaksha, K.J.; Anil, T.M.; Guruchannabasavanna; Patil, P.; Shankar, K.M.; Mohan, C.V.; Sreepada, R.A.

    A monoclonal antibody-based immunodot test was compared to a polymerase chain reaction (PCR) assay for managing white spot syndrome virus (WSSV) on shrimp farms at Kundapur and Kumta situated in Udupi and Uttar Kannada Districts, respectively...

  11. ADHERENCE AND PA THOGENICITY ASSA Y OF VIBRIO HARVE YI IN TIGER SHRIMP (PENAEUS MONODON LARVAE FOR SC REENING BIOCONTROL AGE NT

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    YUSMINAH H ALA

    2002-01-01

    Full Text Available Rifampicin-resistant marker w as empl oyed as a repor ter t o detect the ad here nce and colonization of V. harve yi in s hrimp larvae. Vibri o h arve yi P1B and YA3 2. 2 were isolated f rom dead s hrimp la rv ae i n Besuki, Norther n C oast of East Jav a, while V. harveyi HB3, was isolated from pris tine sea water in Pacita n, S outhe rn Coast of E ast Ja va . Vi brio metsc hnikovii used a s bioc ontr ol a gent was i solated fr om healthy shrimp larvae in Serang, We st Java. Spontane ous mutation was c onducted to generate V. harveyi P1B, YA32.2 and HB3 resistant to rifampi cin. These mutants exhibit ed similar survival ability to t heir parent al (wild type strai ns. Signifi cant larval mortalit y was o bserv ed i n s hrimp l arv ae i noculated with YA32.2 than that of larvae ino culated with P1B. Larvae inoculated with H B 3 showed the lo w est mortality. Bacterial cell c ount of Vi brio Rf* in dead larvae w ere 103-104 cells/larvae. Isolates of Vi brio metschnikovii Z an d M as biocontrol candid ates effectively r educed the growth a nd a dherence ability of YA32.2 t o shrimp larvae. Larval mort ality in rearing water inoculat ed simultaneousl y w ith YA32.2 and V. metschnikovii was lower than the one inoculated with YA32.2 alone. Therefore, Vibrio metschnikovii Z or M could be develope d as an effective pro biotic or biocontrol agent for V. harvey i in shrimp hatcheri es.

  12. Development of a monoclonal antibody-based flow-through immunoassay (FTA) for detection of white spot syndrome virus (WSSV) in black tiger shrimp Penaeus monodon.

    Science.gov (United States)

    Patil, R; Shankar, K M; Kumar, B T N; Kulkarni, A; Patil, P; Moger, N

    2013-09-01

    A flow-through immunoassay (FTA), an improved version of immunodot, was developed using a nitrocellulose membrane baked onto adsorbent pads enclosed in a plastic cassette to detect white spot syndrome virus (WSSV) in shrimp. Sharp purple dots developed with WSSV against the white background of the nitrocellulose membrane. The detection limits of WSSV by the FTA and immunodot were 0.312 and 1.2 μg mL(-1) crude WSSV protein, respectively. The FTA could be completed in 8-10 min compared with 90 min for immunodot. The FTA was 100 times more sensitive than 1-step polymerase chain reaction (PCR) and in between that of the 1- and 2-step PCR protocol recommended by the Office of International Epizootics (OIE). In experimental, orally infected shrimp post-larvae, WSSV was first detected 14, 16 and 18 h post-infection (hpi) by FTA, immunodot and one-step PCR, respectively. The FTA detected WSSV 2 and 4 h earlier than immunodot and one-step PCR, respectively. The FTA was more sensitive (25/27) than one-step PCR (23/27) and immunodot (23/27) for the detection of WSSV from white spot disease outbreak ponds. The reagent components of the FTA were stable giving expected results for 6 m at 4-8 °C. The FTA is available as a rapid test kit called 'RapiDot' for the early detection of WSSV under field conditions. © 2013 John Wiley & Sons Ltd.

  13. Effects of different doses of skt-b vibrio probiotic bacteria addition on survival and growth rate of tiger shrimp (Penaeus monodon larva

    Directory of Open Access Journals (Sweden)

    . Widanarni

    2010-01-01

    Full Text Available Probiotic bacteria has been widely used as biocontrol agents in tiger shrimp hatcheries.  Vibrio SKT-b is one of the probiotic bacteria candidates that could suppressed the growth of pathogenic bacteria Vibrio harveyi and could increase survival rate of tiger shrimp larva. This experiment was carried out to study the effects of probiotic bacteria SKT-b Vibrio addition at different doses on survival and growth rate of tiger shrimp larva.  Experiment was conducted with five treatments and three replications, consisted of SKT-b Vibrio probiotic bacteria addition at the doses of 103 CFU/ml, 104 CFU/ml, 105 CFU/ml, and 106 CFU/ml and control (0 CFU/ml.  Results showed that optimum dose of probiotic bacteria for tiger shrimp was 104 CFU/ml with a survival rate of 94.67%. However, the addition of probiotic bacteria at this particular dose did not significantly increase shrimp growth rate as compared with control. Key words: Probiotic bacteria, SKT-b Vibrio, doses, tiger shrimp larva   ABSTRAK Bakteri probiotik telah banyak digunakan sebagai agen biokontrol dalam pembenihan udang windu.  Vibrio SKT-b merupakan salah satu jenis bakteri kandidat probiotik yang telah diuji dapat menekan pertumbuhan bakteri patogen Vibrio harveyi dan dapat meningkatkan kelangsungan hidup larva udang windu.  Tujuan dari penelitian ini adalah untuk mengetahui pengaruh pemberian bakteri probiotik Vibrio SKT-b dengan dosis yang berbeda terhadap kelangsungan hidup dan pertumbuhan larva udang windu. Penelitian ini dilakukan dalam 5 perlakuan dengan masing-masing 3 ulangan, yaitu penambahan bakteri probiotik Vibrio SKT-b dengan dosis 103 CFU/ml, 104 CFU/ml, 105 CFU/ml, dan 106 CFU/ml dan kontrol (0 CFU/ml.  Hasil penelitian menunjukkan bahwa dosis optimal untuk larva udang windu adalah 104 CFU/ml dengan nilai kelangsungan hidup 94,67%. Namun, pemberian bakteri probiotik tersebut belum menghasilkan pertumbuhan yang berbeda nyata dengan kontrol. Kata kunci: Bakteri probiotik, Vibrio SKT-b, dosis, larva udang windu

  14. Profitability and technical efficiency of Black tiger shrimp (Penaeus Monodon) culture and White leg shrimp (Penaeus Vannamei) culture in Song Song Cau district, Phu Yen province, Vietnam

    OpenAIRE

    Nguyen, Thi Hoai An

    2012-01-01

    The research measure the profitability and technical efficiency of Black tiger shrimp farms and White leg shrimp farms in Song Cau district, Phu Yen province, Vietnam. Cross-sectional data of 62 Black tiger shrimp samples and 88 White leg shrimp samples were used for comparison two production systems. The profitability analysis shows that White leg shrimp farms achieved an average profit per hectare of 78,883,209 VND ($3,944.16), which was approximately 4 times as much as Black tiger shrimp f...

  15. INDUCTION OF GONADAL MATURATION OF POND CULTURED MALE TIGER SHRIMP, Penaeus monodon WITH DIFFERENT DOSAGES OF GONADOTROPIN RELEASING HORMONE ANALOGUE AGAINST EYE STALK ABLATION

    Directory of Open Access Journals (Sweden)

    Asda Laining

    2016-12-01

    Full Text Available Very low naturally mating rate of pond-reared tiger shrimp broodstock is probably due to the slow maturation of the male stock. The aim of this study was to evaluate the salmon gonadotrophin releasing hormone analoque (sGnRHa in stimulating the gonadal maturation of male stock of pond-reared tiger shrimp. The treatments were three dosages of sGnRHa at 0.1 (OV-1, 0.2 (OV-2, and 0.3 (OV-3 mL/kg of shrimp weight and control was eye stalk ablation (AB. The sGnRHa was administered via injection three times with one week interval. Male stocks with average initial body weight of 82.1 g were randomly distributed into four of 10 m3 concrete tanks, 26 males for each tank. Variables observed were performances of spermatophores and profiles of amino acid and fatty acid of muscle of the male stocks. After induction, number of male maturing indicated by spermatophores releasing from terminal ampullas was higher in shrimp induced with OV-1 (80.8% compared to control which was only 46.1%. Furthermore, shrimp treated OV-2 had the highest spermatophore weight of 0.16 g compared to control (0.11 g and other two groups. Amino acid profiles improved as the dose of sGnRHa increased up to 0.2 mL/kg from 61.23% for ablated male becoming 71.27% for OV-2. Total fatty acid also tended to improve by increasing the dose of hormone injection, however, the ablated male had higher total fatty acid content than that of OV-1. The present finding demonstrated that the dose of sGnRHa to stimulate the gonadal maturation of pond-reared male tiger shrimp could be applied at range between 0.1-0.2 mL/kg of shrimp weight.

  16. WSSV risk factors related to water physico-chemical properties and microflora in semi-intensive P .monodon culture ponds in the Philippines

    NARCIS (Netherlands)

    Tendencia Alapide, E.; Bosma, R.H.; Verreth, J.A.J.

    2010-01-01

    Whitespot syndrome virus, WSSV, is the most important among the shrimp diseases. One of the suggested WSSV risk factors is the occurrence of stress since stressors could compromise the shrimp defence system thus increasing the risk of WSSV infection. Stressors are usually related to the

  17. Pengaruh Agency Cost of Free Cash Flow Terhadap Tingkat Konservatisme dan Pengujian Efek Moderasi Kebijakan Hutang, Pendistribusian Kas, Persistensi Kas, dan Tata Kelola Perusahaan

    Directory of Open Access Journals (Sweden)

    Hendro Hendro

    2015-01-01

    Full Text Available This research investigates whether the J-type firm (high agency cost of free cash flow provide more conservative financial statements than non J-type firm. Besides, this research also aim to examine the moderating effects of debt, dividend, stock repurchase, cash persistency, and corporate governance on the relationship between the level of agency cost of free cash flow and conservatism level of financial statements. This research uses two measurement of conservatism, namely accrual conservatism and market value conservatism. Research sample includes manufacturing companies listed in the Indonesian Stock Exchange for the year 2007, 2008 and 2010. The result proves that there is a positive and significant relationship between the level of agency cost of free cash flow and the two measurement of conservatism level of financial stataments. However, this research shows that there is no effect of the moderating variables on that relationship.

  18. EFEKTIVITAS SANKSI PIDANA PAJAK DALAM UNDANG-UNDANG NOMOR 28 TAHUN 2007 TENTANG KETENTUAN UMUM DAN TATA CARA PERPAJAKAN (Studi di Pengadilan Pajak Jakarta

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    Tri Wibowo

    2009-09-01

    Full Text Available One effort of realizing independence of nation in defrayal of development is find the source of fund that coming from tax. But, there are behavior of tax evasion that manipulated by legal subject and object of tax for to get thrift of tax by doing contempt of court (unlawful, and manipulate the tax is coherent virus (inherent in each tax system that applying in every jurisdiction. Based on research result, applying of sanction in the case of manipulate of tax is not yet effective, because not all that order in the norm did, for example bookkeeping’s not true and not yet had NPWP. Therefore, the policy formulation of crime sanctions in Law Taxation and also there must be support society and from government enforcers, so application of sanction of crime in taxation area can effectively did it.

  19. TATA KELOLA PENANGGULANGAN BENCANA ALAM Suatu Deskripsi Inter-Relasi dan Kesiapan Para Pihak dalam rangka Rehabilitasi-Rekonstruksi Rumah Warga Terdampak Pascagempa di Kabupaten Tanah Datar

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    Erwin -

    2015-03-01

    Full Text Available At the time of the earthquake affecting substantial losses due to the damage they cause, usually very high expectations of the people to the government for rehabilitation and reconstruction (rehabilitation and reconstruction of homes affected. Therefore readiness to undertake the rehabilitation of earthquake remains an important concern by the parties in the response to natural disasters, both government and society.  This study is a qualitative study to describe the experience of the parties in order to direct the rehabilitation of houses affected by the earthquake of 2007 in Tanah Datar, West Sumatra province. The results of this study found the application of the principles of transparency, participation and accountability in the interrelation between the government and society in the process of preparation and implementation of the rehabilitation of earthquake-affected neighborhoods. Open governance practices have proven quite successful even minimize complaints and public protests or other social conflict that impacts are not uncommon in post-disaster management in the region. Experiences of good practice organizing the rehabilitation of houses affected by the earthquake in Tanah Datar this would even be used as a pilot, and learning resources of many parties in implementing the governance of disaster management in the various regions

  20. ANALISIS SURVIVAL PENINGKATAN DENSITAS MINERAL TULANG PASIEN PEREMPUAN YANG MENDERITA OSTEOPOROSIS PRIMER DENGAN TERAPI SESUAI TATA LAKSANA KLINIK MTIE (MAKMAL TERPADU IMMUNOENDOKRINOLOGI

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    Halimah Halimah

    2012-11-01

    Full Text Available Background: Life expectacy in Indonesia was increasing every year as impact of access to health services. On 2004 number of elderly people is 16.5 million, 52.6% is female. The most health problem facing by female elderly was osteoporosis that it proved by incidence of osteoporosis among female was higher than male. In fact of that one out of three female tends to have osteoporosis; meanwhile the incidence among male is one out of seven. The objective of this study was to know the length of time for bone development in order to increase the mineral bone density up to 1.5% and 3 percentages in three measurement locations (lumbal, femur, radius. Method: The study has probed as well as the influence factors of bone growth among the osteoporosis patients who were examinated their bone at Klinik Makmal Terpadu Imunoendokrinologi FK UI. This is a longitudinal study with scope in clinical area which include the secondary data analysis form medical record data. The total sample was 52 osteoporosis patients. Analysis survival application is performed for data analysis by using variable time and event from January 2004 to December 2007. The analysis in this study is univariate, bivariate,Kaplan-Meier method, and multivariate with double regresi cox. The factors related with time of remodeling bone were medication standard operating procedure (SOP, and body mass index (BMI. Results: Medication SOP in Klinik Makmal has faster time of remodeling bone and significant result comparing with SOP in other policlinic; on lumbal (event 1.5% and 3% and femur (event 1.5%. Patients with BMI <25 has faster time remodeling bone than patients BMI >25 on femur 1.5%. Contraception group and patient’s age have not enough provided the different time of remodeling bone in those measurement. SOP hazard ratio on lumbal was 3.359, it means patient who receive therapy in Klinik Makmal has 3.36 times chance to have lumbal remodeling bone up to 1.5%. Meanwhile, medication SOP hazard ratio on femur event was 1.5% is 2.182, means patient who receives medication SOP in Klinik Makmal has chance 2.18 times to have femur bone development 1.5% (Radius bone are medication SOP and contraception on development 3% and SOP and age on development 1.5%. However, multivariate result does not show statistic significant on radius bone. Key words: the mineral bone density, osteoporosis, MTIE

  1. EFEKTIVITAS SANKSI PIDANA PAJAK DALAM UNDANG-UNDANG NOMOR 28 TAHUN 2007 TENTANG KETENTUAN UMUM DAN TATA CARA PERPAJAKAN (Studi di Pengadilan Pajak Jakarta

    Directory of Open Access Journals (Sweden)

    Tri Wibowo

    2009-09-01

    Full Text Available One effort of realizing independence of nation in defrayal of development is find the source of fund that coming from tax. But, there are behavior of tax evasion that manipulated by legal subject and object of tax for to get thrift of tax by doing contempt of court (unlawful, and manipulate the tax is coherent virus (inherent in each tax system that applying in every jurisdiction.  Based on research result, applying of sanction in the case of manipulate of tax is not yet effective, because not all that order in the norm did, for example bookkeeping’s not true and not yet had NPWP. Therefore, the policy formulation of crime sanctions in Law Taxation and also there must be support society and from government enforcers, so application of sanction of crime in taxation area can effectively did it.   Kata Kunci : Pajak, Penggelapan Pajak, efektivitas sanksi pidana

  2. Human Mediator Enhances Activator-Facilitated Recruitment of RNA Polymerase II and Promoter Recognition by TATA-Binding Protein (TBP) Independently of TBP-Associated Factors

    OpenAIRE

    Wu, Shwu-Yuan; Zhou, Tianyuan; Chiang, Cheng-Ming

    2003-01-01

    Mediator is a general cofactor implicated in the functions of many transcriptional activators. Although Mediator with different protein compositions has been isolated, it remains unclear how Mediator facilitates activator-dependent transcription, independent of its general stimulation of basal transcription. To define the mechanisms of Mediator function, we isolated two forms of human Mediator complexes (Mediator-P.5 and Mediator-P.85) and demonstrated that Mediator-P.5 clearly functions by e...

  3. MEMPERKUAT FUNGSI DAN PERAN PENGAWASAN BADAN PERMUSYAWARATAN DESA (BPD MELALUI ADVOKASI HAK WARGA ATAS INFORMASI PUBLIK UNTUK MEMBANGUN TATA KELOLA PEMERINTAHAN DESA

    Directory of Open Access Journals (Sweden)

    Rochmad Effendy

    2017-04-01

    Full Text Available Unlike during the New Order era which saw village as an extension of the central government without adequate authority to run the village government, village now has recently become self -governing community equipped with broad authority to manage their own affairs for the sake of realizing people’s welfare. It is an entity of community combined with government. It is community who possesses special governmental organizational structure with unique and vast authority to carry out development programs. In doing so, they handle themselves autonomously based on their own cultural values, their initiatives. As such, the central government has nothing but to respect and concede the uniqueness of its each respective village across the nation. In addition, central government has to allocate state budget to realize social and economic justice for more than 17,000 villages. This is the way the Village Law No 6 2014 has designed which differs dramatically from the previous village law. It has adopted recognition and subsidiarity principle in managing village development. In order this to materialize, Village Council whose membership consists elected representatives from among the villagers prominent figures has been empowered its political roles by means of annulling its inherent legislative authorities. To pave the way, it is positioned outside the formal village government organizational structure which makes it fit to function as representative of villagers civil organization. Given this setting, the Council’s main duty is to fight to people’s interest by preventing themselves from village government vested political interest. To make things better, the law has authorized it to organize a strategic villagers gathering held annually namely Village Meeting. As the key actor of the meeting, the council members has to show off their capacities in political communication as well as public communication to run village democratic governance whose operating values are transparency and accountability. For this to occur, advocating people’s information rights is necessary requirement. By so doing, those values will institutionalize within village government daily operations by establishing Public Information and Archives Operator as required by Public Information Transparency Law No 11 2008 which will bring about good village governance.

  4. Impaired Uptake and/or Utilization of Leucine by Saccharomyces cerevisiae Is Suppressed by the SPT15-300 Allele of the TATA-Binding Protein Gene

    DEFF Research Database (Denmark)

    Baerends, RJ; Qiu, Jin-Long; Rasmussen, Simon

    2009-01-01

    Successful fermentations to produce ethanol require microbial strains that have a high tolerance to glucose and ethanol. Enhanced glucose/ethanol tolerance of the laboratory yeast Saccharomyces cerevisiae strain BY4741 under certain growth conditions as a consequence of the expression of a dominant...... us to examine the effect of expression of the SPT15-300 allele in various yeast species of industrial importance. Expression of SPT15-300 in leucine-prototrophic strains of S. cerevisiae, Saccharomyces bayanus, or Saccharomyces pastorianus (lager brewing yeast), however, did not improve tolerance...... to ethanol on complex rich medium (yeast extract-peptone-dextrose). The enhanced growth of the laboratory yeast strain BY4741 expressing the SPT15-300 mutant allele was seen only on defined media with low concentrations of leucine, indicating that the apparent improved growth in the presence of ethanol...

  5. THE LOST-CITY DAN LOST-SPACE KARENA PERKEMBANGAN PENGEMBANGAN TATA-RUANG KOTA Kasus Koridor Komersial Jalan Tunjungan Kotamadya Surabaya

    Directory of Open Access Journals (Sweden)

    Benny Poerbantanoe

    1999-01-01

    Full Text Available Every town hss its own architectural performance. And architecture is not merely a physical landscape of buildings in a town,, as seen by the observer, but it has a deeper mening, which displays the art of structures and constructions. It is a sequence of buildings along the course of its history (Aldo Rossi, 1980. A Town is a complete work of art, performed by men of urban knowledge. Tehe concept of a town or urban workmanship arises from time to time as a work of art in multiple variations, together and according to spirit of the time, included its aspects of belief and religion. The urban work of art or urban artefact is always related to location, historical events and the spesific urban look. Generally a town has particular rythm and dynamics, at least it does not remain static. Therefore it is quite right, when we say, that a town represents the course of its history and technology during the period of its existence. But when we scrutinise alongthe historical and visual systems, it turns out that so many physical and sociqal spaces have been altered with regard to their quality and quantity, as result mis management ot the physical and social spaces by less careful urban reformists. they may bring about certain inbalancy in the system, destroying public imagination and memory about the identity and message conceived at the time of their construction. At the this may affect a condition, commonly called the lost space. Surabaya is one of the larger towns in Indonesia, which afrtime possessed a number of urban artefacts and workmanship. One of which is the Tunjungan Street, designed and developed by the Gemeente Administration of the Duutch as comercial corriodor of the town, with all its advantages and shortcomings. In spite of the fact that it has never been touched by the hands of the prominent urban planner Mr. Thomas C. Karsten, who has prepared and worked out the architecture of the bigger towns of the former Netherlands Indies like Medan, Jakarta, Bandung, Semarang, Malang and Ujung Pandang with their respective spesific colonial character impression. For the aging generation of the town, Surabaya is called with much regret as the lost city, because of the declining quality of some of its urban artefacts, which once has been their pride and their joy. The abovementioned commercial corridor of Jalan Tunjungan is an example of a lost space Abstract in Bahasa Indonesia : Kota adalah arsitektur. Arsitektur yang bukan sekedar gambar (wujud fisik-visual dari kota yang bisa dilihat saja, melainkan juga sebagai suatu konstruksi. Yaitu konstruksi dari kota sepanjang waktu (Aldo Rossi, 1980. Kota merupakan karya seni yang sempurna, yang dibuat hanya oleh orang-orang yang benar-benar mengerti tentang urban. Konsep kota atau tepatnya urban-artefak sebagai karya seni selalu muncul dan diketemukan dalam bentuk-bentuk yang bervariasi ; dalam segala jaman dan kehidupan sosial-religius. Urban-artefak selalu berkaitan dengan tempat, peristiwa dan wujud-kota. Kota pada umumnya mempunyai sifat dinamis, alias tidak statis. Oleh karena itu tidaklah berlebihan apabila terdapat pernyataan umum yang menyebutkan bahwa; kota itu adalah lambang perjalanan sejarah, teknologi dan jamannya. Namun jika disimak dari sistem-sejarah maupun sistem-visual. Banyaknya ruang fisik dan sosial telah berubah baik secara kwalitas maupun kwantitas, sebagai konsekwensi logis adanya pertumbuhan (perkembangan dan pengembangan dari ruang fisik dan sosial, yang belum dikelola secara benar dan baik. Bisa mengganggu keseimbangan , serta merusak kesan dan memori publik tentang identitas dan citra. Yang akhirnya akan dapat melahirkan apa yang disebut dengan lost space. Surabaya adalah salah satu kota besar di Indonesia, yang memiliki beberapa artefak-urban yang spesifik. Salah satunya yaitu jalan Tunjungan; yang pernah didesain serta dikembangkan pada masa pemerintahan Gemeente, sebagai "koridor komersial" Belanda dengan segala kelebihan dan kekurangannya. Walaupun tidak sampai tersentuh oleh tangan Thomas C. Karsten, seorang planolog Belanda yang berkarakteristik untuk desain kota-kota kolonial di Indonesia sebagaimana kota-kota besar lainnya, seperti; Medan, Jakarta, Bandung, Semarang, Malang, Ujung-pandang. Bagi generasi tua, Surabaya kini kemudian terkenal dengan dengan julukan the lost city, karena menurunnya beberapa kwalitas artefak-urban yang dimilikinya. Dimana salah satunya adalah koridor komersial jalan Tunjungan tersebut diatas. Kata kunci: Koridor jalan Tunjungan, Kotamadya Surabaya, Kota yang hilang (Lost-City, Ruang luar yang mubasir (Lost-space

  6. Exposure of mouse cumulus cell nuclei to porcine ooplasmic extract eliminates TATA box protein binding to chromatin, but has no effect on DNA methylation

    OpenAIRE

    Tong, Guo Qing; Heng, Boon Chin; Ng, Soon Chye

    2006-01-01

    Purpose: The low cloning efficiency with SCNT is due to incomplete or partial reprogramming of the donor somatic cell nuclei after microinjection into the enucleated oocyte. A possible solution may be to initiate nuclear reprogramming prior to SCNT.

  7. TINGKAT PENCAHAYAAN ALAMI PADA TATA LETAK INTERIOR AREA BACA PERPUSTAKAAN Studi Kasus : Ruang Layanan Referensi Perpustakaan DaerahProvinsi Jawa Tengah

    Directory of Open Access Journals (Sweden)

    Sutrati Melissa Malik

    2015-07-01

    Full Text Available pace and light are elements that need to be considered in an interior design library. Space Reference Service Central Java Provincial Library during opening hours turn on the lights as an additional light to illuminate the room, this was done because of the condition of the room to maximize the natural light into the room was minimal. This minimal conditions also influenced the layout of the furniture arrangement bookshelf is in the range of openings so that the incoming light is reduced. The purpose of this study was to measure and analyze Level Natural Lighting Interior Layout In Area Read Space Reference Service. The discussion and analysis in this study is quantitative descriptive. Method of Measuring the level of natural lighting in the room is done on a point of reference services Main Measure that is based on the position where the lamp. (Code TU, namely at point Measure Side opening window light in space and outer space, on each plan space services. (Code TUD and TUL and the Supplementary Measure point in the field of reading the reading table in each room service. (Code TUB. Measuring point is taken at a flat field which is located at the height of 0.75 m or 75 cm above the floor. This flat surface called the working field (Frick, 2008 The results of this study the intensity level of natural light in the space can be further enhanced by optimizing the openings on several sides of the building are not obstructed, Placement Area Read in the reference services optimized by the arrangement of the furniture layout in order to light who entered the area can read more leverage and a standardized 225 lux, furniture layout pattern of structuring a strong influence on a bright light into the space and visual comfort of the space. Results of measurement are mostly located below the minimum threshold standards library reading area that is 225 lux occurs because perletakkan furniture is placed on the side of the light source..

  8. Overexpression screens identify conserved dosage chromosome instability genes in yeast and human cancer

    Science.gov (United States)

    Duffy, Supipi; Fam, Hok Khim; Wang, Yi Kan; Styles, Erin B.; Kim, Jung-Hyun; Ang, J. Sidney; Singh, Tejomayee; Larionov, Vladimir; Shah, Sohrab P.; Andrews, Brenda; Boerkoel, Cornelius F.; Hieter, Philip

    2016-01-01

    Somatic copy number amplification and gene overexpression are common features of many cancers. To determine the role of gene overexpression on chromosome instability (CIN), we performed genome-wide screens in the budding yeast for yeast genes that cause CIN when overexpressed, a phenotype we refer to as dosage CIN (dCIN), and identified 245 dCIN genes. This catalog of genes reveals human orthologs known to be recurrently overexpressed and/or amplified in tumors. We show that two genes, TDP1, a tyrosyl-DNA-phosphdiesterase, and TAF12, an RNA polymerase II TATA-box binding factor, cause CIN when overexpressed in human cells. Rhabdomyosarcoma lines with elevated human Tdp1 levels also exhibit CIN that can be partially rescued by siRNA-mediated knockdown of TDP1. Overexpression of dCIN genes represents a genetic vulnerability that could be leveraged for selective killing of cancer cells through targeting of an unlinked synthetic dosage lethal (SDL) partner. Using SDL screens in yeast, we identified a set of genes that when deleted specifically kill cells with high levels of Tdp1. One gene was the histone deacetylase RPD3, for which there are known inhibitors. Both HT1080 cells overexpressing hTDP1 and rhabdomyosarcoma cells with elevated levels of hTdp1 were more sensitive to histone deacetylase inhibitors valproic acid (VPA) and trichostatin A (TSA), recapitulating the SDL interaction in human cells and suggesting VPA and TSA as potential therapeutic agents for tumors with elevated levels of hTdp1. The catalog of dCIN genes presented here provides a candidate list to identify genes that cause CIN when overexpressed in cancer, which can then be leveraged through SDL to selectively target tumors. PMID:27551064

  9. Identification of a small TAF complex and its role in the assembly of TAF-containing complexes.

    Science.gov (United States)

    Demény, Màté A; Soutoglou, Evi; Nagy, Zita; Scheer, Elisabeth; Jànoshàzi, Agnes; Richardot, Magalie; Argentini, Manuela; Kessler, Pascal; Tora, Laszlo

    2007-03-21

    TFIID plays a role in nucleating RNA polymerase II preinitiation complex assembly on protein-coding genes. TFIID is a multisubunit complex comprised of the TATA box binding protein (TBP) and 14 TBP-associated factors (TAFs). Another class of multiprotein transcriptional regulatory complexes having histone acetyl transferase (HAT) activity, and containing TAFs, includes TFTC, STAGA and the PCAF/GCN5 complex. Looking for as yet undiscovered subunits by a proteomic approach, we had identified TAF8 and SPT7L in human TFTC preparations. Subsequently, however, we demonstrated that TAF8 was not a stable component of TFTC, but that it is present in a small TAF complex (SMAT), containing TAF8, TAF10 and SPT7L, that co-purified with TFTC. Thus, TAF8 is a subunit of both TFIID and SMAT. The latter has to be involved in a pathway of complex formation distinct from the other known TAF complexes, since these three histone fold (HF)-containing proteins (TAF8, TAF10 and SPT7L) can never be found together either in TFIID or in STAGA/TFTC HAT complexes. Here we show that TAF8 is absolutely necessary for the integration of TAF10 in a higher order TFIID core complex containing seven TAFs. TAF8 forms a heterodimer with TAF10 through its HF and proline rich domains, and also interacts with SPT7L through its C-terminal region, and the three proteins form a complex in vitro and in vivo. Thus, the TAF8-TAF10 and TAF10-SPT7L HF pairs, and also the SMAT complex, seem to be important regulators of the composition of different TFIID and/or STAGA/TFTC complexes in the nucleus and consequently may play a role in gene regulation.

  10. Dysregulation of gene expression in the striatum of BACHD rats expressing full-length mutant huntingtin and associated abnormalities on molecular and protein levels.

    Science.gov (United States)

    Yu-Taeger, Libo; Bonin, Michael; Stricker-Shaver, Janice; Riess, Olaf; Nguyen, Hoa Huu Phuc

    2017-05-01

    Huntington disease (HD) is an autosomal dominantly inherited neurodegenerative disorder caused by a CAG repeat expansion in the gene coding for the huntingtin protein (HTT). Mutant HTT (mHTT) has been proposed to cause neuronal dysfunction and neuronal loss through multiple mechanisms. Transcriptional changes may be a core pathogenic feature of HD. Utilizing the Affymetrix platform we performed a genome-wide RNA expression analysis in two BACHD transgenic rat lines (TG5 and TG9) at 12 months of age, both of which carry full-length human mHTT but with different expression levels. By defining the threshold of significance at p < 0.01, we found 1608 genes and 871 genes differentially expressed in both TG5 and TG9 rats when compared to the wild type littermates, respectively. We only chose the highly up-/down-regulated genes for further analysis by setting an additional threshold of 1.5 fold change. Comparing gene expression profiles of human HD brains and BACHD rats revealed a high concordance in both functional and IPA (Ingenuity Pathway Analysis) canonical pathways relevant to HD. In addition, we investigated the causes leading to gene expression changes at molecular and protein levels in BACHD rats including the involvement of polyQ-containing transcription factors TATA box-binding protein (TBP), Sp1 and CBP as well as the chromatin structure. We demonstrate that the BACHD rat model recapitulates the gene expression changes of the human disease supporting its role as a preclinical research animal model. We also show for the first time that TFIID complex formation is reduced, while soluble TBP is increased in an HD model. This finding suggests that mHTT is a competitor instead of a recruiter of polyQ-containing transcription factors in the transcription process in HD. Copyright © 2017 Elsevier Ltd. All rights reserved.

  11. Reference gene validation for qPCR in rat carotid body during postnatal development

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    Carroll John L

    2011-10-01

    Full Text Available Abstract Background The carotid bodies are the main arterial oxygen chemoreceptors in mammals. Afferent neural output from the carotid bodies to brainstem respiratory and cardiovascular nuclei provides tonic input and mediates important protective responses to acute and chronic hypoxia. It is widely accepted that the selection of reference genes for mRNA normalization in quantitative real-time PCR must be validated for a given tissue and set of conditions. This is particularly important for studies in carotid body during early postnatal maturation as the arterial oxygen tension undergoes major changes from fetal to postnatal life, which may affect reference gene expression. In order to determine the most stable and suitable reference genes for the study of rat carotid body during development, six commonly used reference genes, β-actin, RPII (RNA polymerase II, PPIA (peptidyl-proyl-isomerase A, TBP (TATA-box binding protein, GAPDH, and 18s rRNA, were evaluated in two age groups (P0-1 and P14-16 under three environmental oxygen conditions (normoxia, chronic hypoxia and chronic hyperoxia using the three most commonly used software programs, geNorm, NormFinder and BestKeeper. Findings The three programs produced similar results but the reference gene rankings were not identical between programs or experimental conditions. Overall, 18s rRNA was the least stable reference gene for carotid body and, when hyperoxia and/or hypoxia conditions were included, actin was similarly unstable. Conclusions Reference or housekeeping gene expression for qPCR studies of carotid body during postnatal development may vary with developmental stage and environmental conditions. Selection of the best reference gene or combination of reference genes for carotid body development studies should take environmental conditions into account. Two commonly used reference genes, 18s rRNA and actin, may be unsuitable for studies of carotid body maturation, especially if the study

  12. Sustained expression of a neuron-specific isoform of the Taf1 gene in development stages and aging in mice

    International Nuclear Information System (INIS)

    Jambaldorj, Jamiyansuren; Makino, Satoshi; Munkhbat, Batmunkh; Tamiya, Gen

    2012-01-01

    Highlights: ► We identified the mouse homologue of neuron-specific TAF1 (N-Taf1). ► Taf1 mRNA was expressed in most tissues and cell lines. ► N-Taf1 mRNA was expressed in the brain and Neuroblastoma N2a cell lines. ► Taf1 and N-Taf1 showed different expression profile in development stage and aging. -- Abstract: TATA-box binding protein associated factor 1 (TAF1) protein is the largest and the essential component of the TFIID complex in the pathway of RNA polymerase II–mediated gene transcription, and it regulates transcription of a large number of genes related to cell division. The neuron-specific isoform of the TAF1 gene (N-TAF1), which we reported previously, may have an essential role in neurons through transcriptional regulation of many neuron-specific genes. In the present study, we cloned the full-length cDNA that encodes the mouse homologue of N-TAF1 (N-Taf1) protein. By carrying out of real time RT-PCR, we investigated the expression analysis of the N-Taf1 mRNA in mouse tissues and cell lines. As well as the human N-TAF1, the N-Taf1 showed limited expression in the brain and neuroblastoma, whereas Taf1 expressed elsewhere. Furthermore, in mouse embryo head or mouse brain, mRNA expression of TAF1 changes dramatically during development but N-Taf1 showed sustained expression. Our result suggests that the N-Taf1 gene has an important role in non-dividing neuronal cell rather than in cell division and proliferation during neurogenesis.

  13. Identification of valid endogenous control genes for determining gene expression in C6 glioma cell line treated with conditioned medium from adipose-derived stem cell.

    Science.gov (United States)

    Iser, I C; de Campos, R P; Bertoni, A P S; Wink, M R

    2015-10-01

    There is growing evidence that mesenchymal stem cells (MSCs) can be important players in the tumor microenvironment. They can affect the glioma progression through the modulation of different genes. This modulation can be evaluated through a very useful model, treating the tumor cells with MSC-conditioned medium. However, for an accurate and reliable gene expression analysis, normalization of gene expression data against reference genes is a prerequisite. We performed a systematic review in an attempt to find a reference gene to use when analyzing gene expression in C6 glioma cells lines. Considering that we were not able to find a reference gene originated by an appropriate validation, in this study we evaluated candidate genes to be used as reference gene in C6 cells under different treatments with adipose-derived stem cells conditioned medium (CM-ADSCs). β-actin (ACTB); glyceraldehyde-3-phosphate dehydrogenase (GAPDH); hypoxanthine-guanine phosphoribosyltransferase I (HPRT-1); TATA box binding protein (TBP) and beta-2-microglobulin (B2M) were evaluated by real-time reverse transcription PCR (RT-qPCR). The mean Cq, the maximum fold change (MFC) and NormFinder software were used for reference gene evaluation and selection. The GAPDH and ACTB genes have been the most widely used reference genes to normalize among the different investigated genes in our review, however, controversially these genes underwent a substantial variability among the genes evaluated in the present work. Individually, TBP gene was more stable when compared with other genes analyzed and the combination of TBP and HPRT-1 was even more stable. These results evidence the importance of appropriate validation of reference genes before performing qPCR experiments. Besides, our data will contribute with researchers that work analyzing the role of ADSCs in glioma microenvironment through gene expression. Copyright © 2015 Elsevier Masson SAS. All rights reserved.

  14. Sustained expression of a neuron-specific isoform of the Taf1 gene in development stages and aging in mice

    Energy Technology Data Exchange (ETDEWEB)

    Jambaldorj, Jamiyansuren [Department of Pharmacology, Institute of Health Biosciences, Graduate School, The University of Tokushima, Tokushima 770-8503 (Japan); Advanced Molecular Epidemiology Research Institute, Yamagata University Faculty of Medicine, Yamagata 990-9585 (Japan); Central Scientific Research Laboratory, Institute of Medical Sciences, Ulaanbaatar (Mongolia); Makino, Satoshi, E-mail: smakino@genetix-h.com [Molecular Neuroscience Research Center, Shiga University of Medical Science, Otsu 520-2192 (Japan); Munkhbat, Batmunkh [Central Scientific Research Laboratory, Institute of Medical Sciences, Ulaanbaatar (Mongolia); Tamiya, Gen [Advanced Molecular Epidemiology Research Institute, Yamagata University Faculty of Medicine, Yamagata 990-9585 (Japan)

    2012-08-24

    Highlights: Black-Right-Pointing-Pointer We identified the mouse homologue of neuron-specific TAF1 (N-Taf1). Black-Right-Pointing-Pointer Taf1 mRNA was expressed in most tissues and cell lines. Black-Right-Pointing-Pointer N-Taf1 mRNA was expressed in the brain and Neuroblastoma N2a cell lines. Black-Right-Pointing-Pointer Taf1 and N-Taf1 showed different expression profile in development stage and aging. -- Abstract: TATA-box binding protein associated factor 1 (TAF1) protein is the largest and the essential component of the TFIID complex in the pathway of RNA polymerase II-mediated gene transcription, and it regulates transcription of a large number of genes related to cell division. The neuron-specific isoform of the TAF1 gene (N-TAF1), which we reported previously, may have an essential role in neurons through transcriptional regulation of many neuron-specific genes. In the present study, we cloned the full-length cDNA that encodes the mouse homologue of N-TAF1 (N-Taf1) protein. By carrying out of real time RT-PCR, we investigated the expression analysis of the N-Taf1 mRNA in mouse tissues and cell lines. As well as the human N-TAF1, the N-Taf1 showed limited expression in the brain and neuroblastoma, whereas Taf1 expressed elsewhere. Furthermore, in mouse embryo head or mouse brain, mRNA expression of TAF1 changes dramatically during development but N-Taf1 showed sustained expression. Our result suggests that the N-Taf1 gene has an important role in non-dividing neuronal cell rather than in cell division and proliferation during neurogenesis.

  15. Identification of valid reference genes for the normalization of RT qPCR gene expression data in human brain tissue

    Directory of Open Access Journals (Sweden)

    Ravid Rivka

    2008-05-01

    Full Text Available Abstract Background Studies of gene expression in post mortem human brain can contribute to understanding of the pathophysiology of neurodegenerative diseases, including Alzheimer's disease (AD, Parkinson's disease (PD and dementia with Lewy bodies (DLB. Quantitative real-time PCR (RT qPCR is often used to analyse gene expression. The validity of results obtained using RT qPCR is reliant on accurate data normalization. Reference genes are generally used to normalize RT qPCR data. Given that expression of some commonly used reference genes is altered in certain conditions, this study aimed to establish which reference genes were stably expressed in post mortem brain tissue from individuals with AD, PD or DLB. Results The present study investigated the expression stability of 8 candidate reference genes, (ubiquitin C [UBC], tyrosine-3-monooxygenase [YWHAZ], RNA polymerase II polypeptide [RP II], hydroxymethylbilane synthase [HMBS], TATA box binding protein [TBP], β-2-microglobulin [B2M], glyceraldehyde-3-phosphate dehydrogenase [GAPDH], and succinate dehydrogenase complex-subunit A, [SDHA] in cerebellum and medial temporal gyrus of 6 AD, 6 PD, 6 DLB subjects, along with 5 matched controls using RT qPCR (TaqMan® Gene Expression Assays. Gene expression stability was analysed using geNorm to rank the candidate genes in order of decreasing stability in each disease group. The optimal number of genes recommended for accurate data normalization in each disease state was determined by pairwise variation analysis. Conclusion This study identified validated sets of mRNAs which would be appropriate for the normalization of RT qPCR data when studying gene expression in brain tissue of AD, PD, DLB and control subjects.

  16. Gene expression results in lipopolysaccharide-stimulated monocytes depend significantly on the choice of reference genes

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    Øvstebø Reidun

    2010-05-01

    Full Text Available Abstract Background Gene expression in lipopolysaccharide (LPS-stimulated monocytes is mainly studied by quantitative real-time reverse transcription PCR (RT-qPCR using GAPDH (glyceraldehyde 3-phosphate dehydrogenase or ACTB (beta-actin as reference gene for normalization. Expression of traditional reference genes has been shown to vary substantially under certain conditions leading to invalid results. To investigate whether traditional reference genes are stably expressed in LPS-stimulated monocytes or if RT-qPCR results are dependent on the choice of reference genes, we have assessed and evaluated gene expression stability of twelve candidate reference genes in this model system. Results Twelve candidate reference genes were quantified by RT-qPCR in LPS-stimulated, human monocytes and evaluated using the programs geNorm, Normfinder and BestKeeper. geNorm ranked PPIB (cyclophilin B, B2M (beta-2-microglobulin and PPIA (cyclophilin A as the best combination for gene expression normalization in LPS-stimulated monocytes. Normfinder suggested TBP (TATA-box binding protein and B2M as the best combination. Compared to these combinations, normalization using GAPDH alone resulted in significantly higher changes of TNF-α (tumor necrosis factor-alpha and IL10 (interleukin 10 expression. Moreover, a significant difference in TNF-α expression between monocytes stimulated with equimolar concentrations of LPS from N. meningitides and E. coli, respectively, was identified when using the suggested combinations of reference genes for normalization, but stayed unrecognized when employing a single reference gene, ACTB or GAPDH. Conclusions Gene expression levels in LPS-stimulated monocytes based on RT-qPCR results differ significantly when normalized to a single gene or a combination of stably expressed reference genes. Proper evaluation of reference gene stabiliy is therefore mandatory before reporting RT-qPCR results in LPS-stimulated monocytes.

  17. In search of suitable reference genes for gene expression studies of human renal cell carcinoma by real-time PCR

    Directory of Open Access Journals (Sweden)

    Kristiansen Glen

    2007-06-01

    Full Text Available Abstract Background Housekeeping genes are commonly used as endogenous reference genes for the relative quantification of target genes in gene expression studies. No conclusive systematic study comparing the suitability of different candidate reference genes in clear cell renal cell carcinoma has been published to date. To remedy this situation, 10 housekeeping genes for normalizing purposes of RT-PCR measurements already recommended in various studies were examined with regard to their usefulness as reference genes. Results The expression of the potential reference genes was examined in matched malignant and non-malignant tissue specimens from 25 patients with clear cell renal cell carcinoma. Quality assessment of isolated RNA performed with a 2100 Agilent Bioanalyzer showed a mean RNA integrity number of 8.7 for all samples. The between-run variations related to the crossing points of PCR reactions of a control material ranged from 0.17% to 0.38%. The expression of all genes did not depend on age, sex, and tumour stage. Except the genes TATA box binding protein (TBP and peptidylprolyl isomerase A (PPIA, all genes showed significant differences in expression between malignant and non-malignant pairs. The expression stability of the candidate reference genes was additionally controlled using the software programs geNorm and NormFinder. TBP and PPIA were validated as suitable reference genes by normalizing the target gene ADAM9 using these two most stably expressed genes in comparison with up- and down-regulated housekeeping genes of the panel. Conclusion Our study demonstrated the suitability of the two housekeeping genes PPIA and TBP as endogenous reference genes when comparing malignant tissue samples with adjacent normal tissue samples from clear cell renal cell carcinoma. Both genes are recommended as reference genes for relative gene quantification in gene profiling studies either as single gene or preferably in combination.

  18. Stability of Reference Genes for Messenger RNA Quantification by Real-Time PCR in Mouse Dextran Sodium Sulfate Experimental Colitis.

    Directory of Open Access Journals (Sweden)

    Nour Eissa

    Full Text Available Many animal models have been developed to characterize the complexity of colonic inflammation. In dextran sodium sulfate (DSS experimental colitis in mice the choice of reference genes is critical for accurate quantification of target genes using quantitative real time PCR (RT-qPCR. No studies have addressed the performance of reference genes in mice DSS-experimental colitis. This study aimed to determine the stability of reference genes expression (RGE in DSS-experimental murine colitis.Colitis was induced in male C57BL/6 mice using DSS5% for 5 days, control group received water. RNA was extracted from inflamed and non-inflamed colon. Using RT-qPCR, comparative analysis of 13 RGE was performed according to predefined criteria and relative colonic TNF-α and IL-1β gene expression was determined by calculating the difference in the threshold cycle.Colitis significantly altered the stability of mucosal RGE. Commonly used glyceraldehyde-3-phosphate dehydrogenase (Gapdh, β-actin (Actb, or β2-microglobulin (β2m showed the highest variability within the inflamed and control groups. Conversely, TATA-box-binding protein (Tbp and eukaryotic translation elongation factor 2 (Eef2 were not affected by inflammation and were the most stable genes. Normalization of colonic TNF-α and IL-1β mRNA levels was dependent on the reference gene used. Depending on the genes used to normalize the data, statistical significance varied from significant when TBP / Eef2 were used to non-significant when Gapdh, Actb or β2m were used.This study highlights the appropriate choice of RGE to ensure adequate normalization of RT-qPCR data when using this model. Suboptimal RGE may explain controversial results from published studies. We recommend using Tbp and Eef2 instead of Gapdh, Actb or β2m as reference genes.

  19. Low-pass sequencing for microbial comparative genomics

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    Kennedy Sean

    2004-01-01

    Full Text Available Abstract Background We studied four extremely halophilic archaea by low-pass shotgun sequencing: (1 the metabolically versatile Haloarcula marismortui; (2 the non-pigmented Natrialba asiatica; (3 the psychrophile Halorubrum lacusprofundi and (4 the Dead Sea isolate Halobaculum gomorrense. Approximately one thousand single pass genomic sequences per genome were obtained. The data were analyzed by comparative genomic analyses using the completed Halobacterium sp. NRC-1 genome as a reference. Low-pass shotgun sequencing is a simple, inexpensive, and rapid approach that can readily be performed on any cultured microbe. Results As expected, the four archaeal halophiles analyzed exhibit both bacterial and eukaryotic characteristics as well as uniquely archaeal traits. All five halophiles exhibit greater than sixty percent GC content and low isoelectric points (pI for their predicted proteins. Multiple insertion sequence (IS elements, often involved in genome rearrangements, were identified in H. lacusprofundi and H. marismortui. The core biological functions that govern cellular and genetic mechanisms of H. sp. NRC-1 appear to be conserved in these four other halophiles. Multiple TATA box binding protein (TBP and transcription factor IIB (TFB homologs were identified from most of the four shotgunned halophiles. The reconstructed molecular tree of all five halophiles shows a large divergence between these species, but with the closest relationship being between H. sp. NRC-1 and H. lacusprofundi. Conclusion Despite the diverse habitats of these species, all five halophiles share (1 high GC content and (2 low protein isoelectric points, which are characteristics associated with environmental exposure to UV radiation and hypersalinity, respectively. Identification of multiple IS elements in the genome of H. lacusprofundi and H. marismortui suggest that genome structure and dynamic genome reorganization might be similar to that previously observed in the

  20. Effect of obesity and exercise on the expression of the novel myokines, Myonectin and Fibronectin type III domain containing 5

    Directory of Open Access Journals (Sweden)

    Jonathan M. Peterson

    2014-09-01

    obesity and/or exercise and would have skewed the results of this study if used to normalize gene expression data. The unstable reference genes include: beta-Actin, beta-2-microglobulin, Non-POU domain containing, octamer-binding, Peptidylprolyl isomerase H, 18S ribosomal RNA, TATA box binding protein and Transferrin receptor.

  1. A hierarchical analysis of transcriptome alterations in intrauterine growth restriction (IUGR) reveals common pathophysiological pathways in mammals.

    Science.gov (United States)

    Buffat, C; Mondon, F; Rigourd, V; Boubred, F; Bessières, B; Fayol, L; Feuerstein, J-M; Gamerre, M; Jammes, H; Rebourcet, R; Miralles, F; Courbières, B; Basire, A; Dignat-Georges, F; Carbonne, B; Simeoni, U; Vaiman, D

    2007-11-01

    Intra-uterine growth restriction (IUGR) is a frequent disease, affecting up to 10% of human pregnancies and responsible for increased perinatal morbidity and mortality. Moreover, low birth weight is an important cause of the metabolic syndrome in the adult. Protein depletion during the gestation of rat females has been widely used as a model for human IUGR. By transcriptome analysis of control and protein-deprived rat placentas, we were able to identify 2543 transcripts modified more than 2.5 fold (1347 induced and 1196 repressed). Automatic functional classification enabled us to identify clusters of induced genes affecting chromosome structure, transcription, intracellular transport, protein modifications and apoptosis. In particular, we suggest the existence of a complex balance regulating apoptosis. Among repressed genes, we noted several groups of genes involved in immunity, signalling and degradation of noxious chemicals. These observations suggest that IUGR placentas have a decreased resistance to external aggression. The promoters of the most induced and most repressed genes were contrasted for their composition in putative transcription factor binding sites. There was an over-representation of Zn finger (ZNF) proteins and Pdx1 (pancreatic and duodenal homeobox protein 1) putative binding sites. Consistently, Pdx1 and a high proportion of ZNF genes were induced at the transcriptional level. A similar analysis of ZNF promoters showed an increased presence of putative binding sites for the Tata box binding protein (Tbp). Consistently again, we showed that the Tbp and TBP-associated factors (Tafs) were up-regulated in IUGR placentas. Also, samples of human IUGR and control placentas showed that human orthologous ZNFs and PDX1 were transcriptionally induced, especially in non-vascular IUGR. Immunohistochemistry revealed increased expression of PDX1 in IUGR human placentas. In conclusion, our approach permitted the proposition of hypotheses on a hierarchy of

  2. Selection of reference genes for qPCR in hairy root cultures of peanut

    Directory of Open Access Journals (Sweden)

    Medrano Giuliana

    2011-10-01

    Full Text Available Abstract Background Hairy root cultures produced via Agrobacterium rhizogenes-mediated transformation have emerged as practical biological models to elucidate the biosynthesis of specialized metabolites. To effectively understand the expression patterns of the genes involved in the metabolic pathways of these compounds, reference genes need to be systematically validated under specific experimental conditions as established by the MIQE (Minimum Information for Publication of Quantitative Real-Time PCR Experiments guidelines. In the present report we describe the first validation of reference genes for RT-qPCR in hairy root cultures of peanut which produce stilbenoids upon elicitor treatments. Results A total of 21 candidate reference genes were evaluated. Nineteen genes were selected based on previous qPCR studies in plants and two were from the T-DNAs transferred from A. rhizogenes. Nucleotide sequences of peanut candidate genes were obtained using their homologous sequences in Arabidopsis. To identify the suitable primers, calibration curves were obtained for each candidate reference gene. After data analysis, 12 candidate genes meeting standard efficiency criteria were selected. The expression stability of these genes was analyzed using geNorm and NormFinder algorithms and a ranking was established based on expression stability of the genes. Candidate reference gene expression was shown to have less variation in methyl jasmonate (MeJA treated root cultures than those treated with sodium acetate (NaOAc. Conclusions This work constitutes the first effort to validate reference genes for RT-qPCR in hairy roots. While these genes were selected under conditions of NaOAc and MeJA treatment, we anticipate these genes to provide good targets for reference genes for hairy roots under a variety of stress conditions. The lead reference genes were a gene encoding for a TATA box binding protein (TBP2 and a gene encoding a ribosomal protein (RPL8C. A

  3. Characterization of housekeeping genes in zebrafish: male-female differences and effects of tissue type, developmental stage and chemical treatment

    Directory of Open Access Journals (Sweden)

    Callard Gloria V

    2008-11-01

    Full Text Available Abstract Background Research using the zebrafish model has experienced a rapid growth in recent years. Although real-time reverse transcription PCR (QPCR, normalized to an internal reference ("housekeeping" gene, is a frequently used method for quantifying gene expression changes in zebrafish, many commonly used housekeeping genes are known to vary with experimental conditions. To identify housekeeping genes that are stably expressed under different experimental conditions, and thus suitable as normalizers for QPCR in zebrafish, the present study evaluated the expression of eight commonly used housekeeping genes as a function of stage and hormone/toxicant exposure during development, and by tissue type and sex in adult fish. Results QPCR analysis was used to quantify mRNA levels of bactin1, tubulin alpha 1(tuba1, glyceraldehyde-3-phosphate dehydrogenase (gapdh, glucose-6-phosphate dehydrogenase (g6pd, TATA-box binding protein (tbp, beta-2-microglobulin (b2m, elongation factor 1 alpha (elfa, and 18s ribosomal RNA (18s during development (2 – 120 hr postfertilization, hpf; in different tissue types (brain, eye, liver, heart, muscle, gonads of adult males and females; and after treatment of embryos/larvae (24 – 96 hpf with commonly used vehicles for administration and agents that represent known environmental endocrine disruptors. All genes were found to have some degree of variability under the conditions tested here. Rank ordering of expression stability using geNorm analysis identified 18s, b2m, and elfa as most stable during development and across tissue types, while gapdh, tuba1, and tpb were the most variable. Following chemical treatment, tuba1, bactin1, and elfa were the most stably expressed whereas tbp, 18s, and b2m were the least stable. Data also revealed sex differences that are gene- and tissue-specific, and treatment effects that are gene-, vehicle- and ligand-specific. When the accuracy of QPCR analysis was tested using

  4. Journal of Biosciences | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    2 Manda C Purandare3 A N Bhisey1. Cancer Research Institute, Parel, Mumbai 400 012, India; Tata Memorial Hospital, Tata Memorial Centre, Parel, Mumbai 400 012, India; Nowrosjee Wadia Maternity Hospital, Parel, Mumbai 400 012, India ...

  5. Prevalence of Intestinal Parasites in Primary School Children of ...

    African Journals Online (AJOL)

    Research, and Education in Cancer,. Tata Memorial ... in Cancer, Tata Memorial Center, Navi Mumbai, India .... parasites and socioeconomic status determined using the Chi ..... The impact of helminths on the response ... Municipality, Kenya.

  6. Proses Komunikasi Antar Pribadi Dalam Keluarga Ibu Bekerja (Studi Deskriptif Kualitatif mengenai Proses Komunikasi Antar Pribadi Ibu Bekerja pada Subbagian Tata Laksana dan Kepegawaian, Biro Umum, Sekjen Kemendikbud Jakarta dengan Suami dan Anak dalam Hubungan Harmonisasi)

    OpenAIRE

    Putri, Melati Wanda

    2016-01-01

    This study titled Interpersonal Communication Process in the Mother's Work Family. A qualitative descriptive study aimed to determine the process of interpersonal communication that takes place between mother worked with her husband and children in the harmonization of the relationship as well as to find out the scheme in family relationships. The theory used in this study is the Theory of Communication, Interpersonal Communication Theory, Theory of Self-Disclosure, and Family Communication. ...

  7. Kajian Tentang Perilaku Dysfungsional Dalam Keterkaitan Dengan Anggaran, Struktur Organisasi Dan Tata Kerja, Dan Budaya Nasional Dengan Tiga Dimensi (Power Distance, Materialism Dan Femininism) (Penelitian Pada Satuan Kerja Perangkat Daerah Pemerintah Dae

    OpenAIRE

    Lili Sugeng Wiyantoro; Agus Solikhan Yulianto

    2012-01-01

    This study examines the effect of budgetary participation, reliance on accounting performance measures, standard operating procedure of local government toward dysfunctional behavior, to examines the effect of national culture toward dysfunctional behavior and to examines three dimensions of national cultures (power distance, materialism, and feminism) as moderating variable that effect of relationship between budgetary participation, reliance on accounting performance measures, and standard ...

  8. TATA CARA PELAKSANAAN SHALAT JUM'AT (Studi Naskah “Sulûk Al-Jâddah Fî Bayân Al-Jum’ah” Karya Syeikh Nawawi al-Bantani

    Directory of Open Access Journals (Sweden)

    Masrukhin Muhsin

    2012-07-01

    Full Text Available Makalah ini merupakan hasil penelitian naskah “Sulûk al-Jâddah Fî Bayân al-Jum’ah” Karya Syeikh Nawawi al-Bantani. Penelitian ini menggunakan pendekatan filologi. Dalam penelitian filologi dikenal dua perlakuan terhadap naskah. Pertama, memperlakukan satu naskah sebagai bagian dari naskah-naskah lainnya yang sejudul. Dalam hal ini semua naskah yang sejudul dikumpulkan di manapun adanya, dengan tujuan mendapatkan naskah asli atau dianggap paling mendekati asli. Kedua, memperlakukan naskah sebagai naskah tunggal. Dalam hal ini peneliti mengesampingkan naskah lain yang kemungkinan ada di tempat lain. Dari dua model tersebut, penelitian ini menggunakan model kedua. Alasannya, naskah Sulûk al-Jâddah fî Bayân al- Jum’ah untuk sementara dinyatakan sebagai naskah tunggal dengan indikasi tidak ditemukan naskah lain. Untuk menganalisa data naskah, dilakukan pembacaan dua tahap, heuristik dan hermeneutik. Adapun pokok-pokok bahasan yang ada dalam naskah Sulûk al-Jâddah fî Bayân al-Jum’ah adalah berisi tentang masalah shalat Jumát dan permasalahan-perasalahan yang dihadapi oleh masyarakat seputar shalat Jum’at dan shalat Jum’at yang diulang.

  9. PEMBATASAN HAK KASASI DAN KONSEKUENSI HUKUM BAGI PENCARI KEADILAN DALAM SISTEM PERADILAN TATA USAHA NEGARA DI INDONESIA / The Restriction of Cassation Right and the Consequence for Justice Seeker in Indonesian Administrative Justice System

    Directory of Open Access Journals (Sweden)

    Agus Budi Susilo

    2016-07-01

    There were appeal legal effort, cassation and judicial review on Administrative Court. After the Supreme Court Act article 45A paragraph (2 letter c was applied, it was determined that not all administrative settlement dispute can be filled to cassation legal effort. The setting restriction poses legal problems to justice seekers. This article aims to study the solution of cassation rights setting restrictions so that it can be mutual for administrative justice seekers. Based on the analysis that has been done it can be concluded that the regulation on Supreme Court Act article 45A paragraph (2 letter c Act number 5 2014 was not clear in procedures and substantive. Thus the setting restriction in cassation legal effort has to consider the aspect of quality and cases type.

  10. Evolutionarily Conserved, Growth Plate Zone-Specific Regulation of the Matrilin-1 Promoter: L-Sox5/Sox6 and Nfi Factors Bound near TATA Finely Tune Activation by Sox9 ▿

    Science.gov (United States)

    Nagy, Andrea; Kénesi, Erzsébet; Rentsendorj, Otgonchimeg; Molnár, Annamária; Szénási, Tibor; Sinkó, Ildikó; Zvara, Ágnes; Thottathil Oommen, Sajit; Barta, Endre; Puskás, László G.; Lefebvre, Veronique; Kiss, Ibolya

    2011-01-01

    To help uncover the mechanisms underlying the staggered expression of cartilage-specific genes in the growth plate, we dissected the transcriptional mechanisms driving expression of the matrilin-1 gene (Matn1). We show that a unique assembly of evolutionarily conserved cis-acting elements in the Matn1 proximal promoter restricts expression to the proliferative and prehypertrophic zones of the growth plate. These elements functionally interact with distal elements and likewise are capable of restricting the domain of activity of a pancartilaginous Col2a1 enhancer. The proximal elements include a Pe1 element binding the chondrogenic L-Sox5, Sox6, and Sox9 proteins, a SI element binding Nfi proteins, and an initiator Ine element binding the Sox trio and other factors. Sox9 binding to Pe1 is indispensable for functional interaction with the distal promoter. Binding of L-Sox5/Sox6 to Ine and Nfib to SI modulates Sox9 transactivation in a protein dose-dependent manner, possibly to enhance Sox9 activity in early stages of chondrogenesis and repress it at later stages. Hence, our data suggest a novel model whereby Sox and Nfi proteins bind to conserved Matn1 proximal elements and functionally interact with each other to finely tune gene expression in specific zones of the cartilage growth plate. PMID:21173167

  11. Penerapan e-Procurement Dalam Proses Pengadaan Barang Dan Jasa Pemerintah Guna Mendukung Ketahanan Tata Pemerintahan Daerah (Studi pada Unit Layanan Pengadaan Barang dan Jasa Pemerintah Kabupaten Penajam Paser Utara Provinsi Kalimantan Timur)

    OpenAIRE

    Hidayat, Rahmat

    2015-01-01

    ABSTRACTThe purpose of this study was to analyzed the application of the principle of e-Procurement in ProcurementServices Unit in the Government District Penajam Paser Utara, examines the obstacles faced by the ProcurementServices Unit in the process procurement of goods and services by the government through e-procurement process andto formulated strategies that were done overcome by Procurement Services Unit to the obstacles in implementation of e-procurement to supported the resilience of...

  12. Fulltext PDF

    Indian Academy of Sciences (India)

    White Spot Syndrome Virus infection in Penaeus monodon is facilitated by .... Down-regulation of human cytomegalovirus UL138, a novel latency-associated .... Effect of normabaric hyperoxia treatment on neuronal damage following fluid ...

  13. Efficacy of micro algae and cyanobacteria as a live feed for juveniles ...

    African Journals Online (AJOL)

    Administrator

    2011-09-21

    Sep 21, 2011 ... of feeding the juvenile with micro algal diets and cyanobacterial diets at the same concentrations. ... Key words: Penaeus monodon, micro algae, Synechococcus, Phormidium, ..... developments in cultivation and utilization.

  14. Virus diseases risk-factors associated with shrimp farming practices in rice-shrimp and intensive culture systems in Mekong Delta Viet Nam

    NARCIS (Netherlands)

    Duc, P.M.; Tuyet Hoa, T.T.; Nguyen Thanh Phuong,; Bosma, R.H.; Huynh V., Hien; Tran N., Tuan

    2015-01-01

    In Mekong Delta, viral infection, including white spot syndrome virus (WSSV), monodon baculovirus (MBV), heptopancreatic parvovirus (HPV), infectious hypodermal and hematopoietic necrosis virus (IHHNV) and gill-associated nidovirus (GAV) frequently infect cultured shrimp starting at the postlarvae

  15. 2633-IJBCS-Article-Luc Gangbe

    African Journals Online (AJOL)

    hp

    Biology and breeding prospect of giant fresh water shrimp Macrobrachium vollenhovenii (Herklots, 1857) ... give the hatch small larvae called nauplius that evolves zoe and mysis. After 15 larval stages, ...... monodon, Litopenaeus vannamei et.

  16. Biochemical evaluation of aestivation and starvation in two snail ...

    African Journals Online (AJOL)

    hope&shola

    2010-11-08

    Nov 8, 2010 ... ned by drying up of surface water from fresh water bodies either regularly in ... transferases; LDL, low-density lipoprotein; BUN, blood urea nitrogen; UUN ..... white spot syndrome virus infection in penaeus monodon (fabricius).

  17. Main: 1QN6 [RPSD[Archive

    Lifescience Database Archive (English)

    Full Text Available itiation Factor Tfiid-1; Chain: A, B; Synonym: Tata-Box Factor 1, Tata Sequence-Binding Protein 1, Tbp1; Eng...ineered: Yes; Molecule: Dna (5'-D(*Gp*Cp*Tp*Ap*Tp*Ap*Ap*Tp*Ap*Gp*Gp*Gp*Cp*A)-3'); Chai...n: C, E; Molecule: Dna (5'-D(*Tp*Gp*Cp*Cp*Cp*Tp*Ap*Tp*Tp*Ap*Tp*Ap*Gp*C)-3'); Chain: D, F Tata Box-Bindi

  18. Main: 1QNB [RPSD[Archive

    Lifescience Database Archive (English)

    Full Text Available itiation Factor Tfiid-1; Chain: A, B; Synonym: Tata-Box Factor 1, Tata Sequence-Binding Protein 1, Tbp1; Eng...ineered: Yes; Molecule: Dna (5'-D(*Gp*Cp*Tp*Ap*Tp*Ap*Ap*Ap*Tp*Gp*Gp*Gp*Cp*A)-3'); Chai...n: C, E; Molecule: Dna (5'-D(*Tp*Gp*Cp*Cp*Cp*Ap*Tp*Tp*Tp*Ap*Tp*Ap*Gp*C)-3'); Chain: D, F Tata Box-Bindi

  19. Main: 1QN5 [RPSD[Archive

    Lifescience Database Archive (English)

    Full Text Available itiation Factor Tfiid-1; Chain: A, B; Synonym: Tata-Box Factor 1, Tata Sequence-Binding Protein 1, Tbp1; Eng...ineered: Yes; Molecule: Dna (5'-D(*Gp*Cp*Tp*Ap*Tp*Ap*Ap*Gp*Ap*Gp*Gp*Gp*Cp*A)-3'); Chai...n: C, E; Molecule: Dna (5'-D(*Tp*Gp*Cp*Cp*Cp*Tp*Cp*Tp*Tp*Ap*Tp*Ap*Gp*C)-3'); Chain: D, F Tata Box-Bindi

  20. Main: 1QNA [RPSD[Archive

    Lifescience Database Archive (English)

    Full Text Available itiation Factor Tfiid-1; Chain: A, B; Synonym: Tata-Box Factor 1, Tata Sequence-Binding Protein 1, Tbp1; Eng...ineered: Yes; Molecule: Dna (5'-D(*Gp*Cp*Tp*Tp*Tp*Ap*Ap*Ap*Ap*Gp*Gp*Gp*Cp*A)-3'); Chai...n: C, E; Molecule: Dna (5'-D(*Tp*Gp*Cp*Cp*Cp*Tp*Tp*Tp*Tp*Ap*Ap*Ap*Gp*C)-3'); Chain: D, F Tata Box-Bindi

  1. 77 FR 47882 - Notice Pursuant to the National Cooperative Research and Production Act of 1993-Cooperative...

    Science.gov (United States)

    2012-08-10

    ...; Navistar, Inc., Melrose Park, IL; Tata Motors, Ltd., Mumbai, INDIA; Toyota Motor Corp., Shizuoka, JAPAN..., Inc., Torrance, CA; Isuzu Motors Limited, Fujisawa, JAPAN; Jacobs Vehicle Systems, Bloomfield, CT...

  2. The Relationship Between Library Users' Perception And Satisfaction On Library Services At The Center Of Information And Library Resources (Cisral Of Padjadjaran University

    Directory of Open Access Journals (Sweden)

    - Damayanti

    2016-11-01

    tentang fasilitas dan tata ruang layanan referensi dengan kepuasan pemustaka pada bagian layanan referensi di CISRAL Unpad berhubungan sangat kuat. Kata Kunci: persepsi pemustaka, kepuasan pemustaka, layanan perpustakaan.

  3. Cryptococcus

    Indian Academy of Sciences (India)

    Vikas Yadav

    The Molecular Mycology Laboratory. CSIR, DBT, SERB,. CEFIPRA (Indo-French), IJSPS (Indo-Japan). JNCASR (intramural). TATA innovation Fellowship. Rahul Siddharthan. DD Dubey. German Larriba. Joseph Heitman.

  4. Perencanaan dan Implementasi Information Security Management System Menggunakan Framework ISO/IEC 20071

    Directory of Open Access Journals (Sweden)

    Anggi Anugraha Putra

    2016-01-01

    Full Text Available Penerapan tata kelola Teknologi Informasi saat ini sudah menjadi kebutuhan dan tuntutan di setiap instansi penyelenggara pelayanan publik mengingat peran TI yang semakin penting bagi upaya peningkatan kualitas layanan sebagai salah satu realisasi dari tata kelola pemerintahan yang baik (Good Corporate Governance. Dalam penyelenggaraan tata kelola TI, faktor keamanan informasi merupakan aspek yang sangat penting diperhatikan mengingat kinerja tata kelola TI akan terganggu jika informasi sebagai salah satu objek utama tata kelola TI mengalami masalah keamanan informasi yang menyangkut kerahasiaan (confidentiality, keutuhan (integrity dan ketersediaan (availability. Information Security Management System (ISMS adalah seperangkat kebijakan berkaitan dengan manajemen keamanan informasi atau terkait dengan risiko TI. Prinsip yang mengatur di balik ISMS adalah bahwa organisasi harus merancang, menerapkan dan memelihara seperangkat kebijakan, proses dan sistem untuk mengelola risiko aset informasi mereka, sehingga memastikan tingkat risiko keamanan informasi yang dapat diterima. Dari perencanaan dan implementasi sistem manajemen keamanan informasi ini, dihasilkan daftar nilai risiko akhir aset- aset kritikal dan dokumen-dokumen tata kelola penunjang ISMS. Metode penelitian yang digunakan adalah studi kasus yang didalam hal ini, merupakan penelitian kualitatif. Adapun proses yang digunakan untuk mengukur tingkat kematangan dari tata kelola keamanan sistem informasi ini berdasarkan kerangka kerja ISO/IEC 27001. Dari kerangka tersebut kemudian dilakukan evaluasi terhadap objek kontrol yang dimiliki ISO/IEC 27001. Hasil yang didapat adalah peningkatan terhadap tata kelola keamanan sistem informasi. Kesimpulan dari penelitian ini adalah dibutuhkannya tata kelola keamanan sistem informasi agar IT dapat diandalkan untuk mencapai tujuan bisnis.

  5. Tole, Dr Shubha

    Indian Academy of Sciences (India)

    . Date of birth: 31 August 1967. Specialization: Neuroscience, Developmental Biology Address: Professor, Department of Biological Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400 005, Maharashtra

  6. Regulation of methane genes and genome expression

    Energy Technology Data Exchange (ETDEWEB)

    John N. Reeve

    2009-09-09

    , designated TFE, that had sequences in common with the eukaryotic general transcription factor TFIIE, stimulated archaeal transcription initiation and that the archaeal TATA-box binding protein (TBP) remained attached to the promoter region whereas the transcription factor TFB dissociated from the template DNA following initiation. DNA sequences that directed the localized assembly of archaeal histones into archaeal nucleosomes were identified, and we established that transcription by an archaeal RNA polymerase was slowed but not blocked by archaeal nucleosomes. We developed a new protocol to purify archaeal RNA polymerases and with this enzyme and additional improvements to the in vitro transcription system, we established the template requirements for archaeal transcription termination, investigated the activities of proteins predicted to be methane gene regulators, and established how TrpY, a novel archaeal regulator of expression of the tryptophan biosynthetic operon functions in M. thermautotrophicus. This also resulted in the discovery that almost all M. thermautotrophicus mutants isolated as spontaneously resistant to 5-methyl tryptophan (5MTR) had mutations in trpY and were therefore 5MTR through de-repressed trp operon expression. This established a very simple, practical procedure to determine and quantify the DNA sequence changes that result from exposure of this Archaeon to any experimental mutagenesis protocol. Following the discovery that the Thermococcus kodakaraensis was amenable to genetic manipulation, we established this technology at OSU and subsequently added plasmid expression, a reporter system and additional genetic selections to the T. kodakaraensis genetic toolbox. We established that transcription and translation are coupled in this Archaeon, and by combining in vitro transcription and in vivo genetics, we documented that both TFB1 and TFB2 support transcription initiation in T. kodakaraensis. We quantified the roles of ribosome binding sequences

  7. Yeast Interacting Proteins Database: YGL237C, YOR047C [Yeast Interacting Proteins Database

    Lifescience Database Archive (English)

    Full Text Available ene expression; interacts with protein kinase Snf1p, glucose sensors Snf3p and Rgt2p, and TATA-binding prote... expression; interacts with protein kinase Snf1p, glucose sensors Snf3p and Rgt2p, and TATA-binding protein

  8. Yeast Interacting Proteins Database: YOR358W, YOR047C [Yeast Interacting Proteins Database

    Lifescience Database Archive (English)

    Full Text Available ; interacts with protein kinase Snf1p, glucose sensors Snf3p and Rgt2p, and TATA-binding protein Spt15p; act...rotein kinase Snf1p, glucose sensors Snf3p and Rgt2p, and TATA-binding protein Spt15p; acts as a regulator o

  9. Yeast Interacting Proteins Database: YKL002W, YOR047C [Yeast Interacting Proteins Database

    Lifescience Database Archive (English)

    Full Text Available ene expression; interacts with protein kinase Snf1p, glucose sensors Snf3p and Rgt2p, and TATA-binding prote...xpression; interacts with protein kinase Snf1p, glucose sensors Snf3p and Rgt2p, and TATA-binding protein Sp

  10. Yeast Interacting Proteins Database: YLR447C, YOR047C [Yeast Interacting Proteins Database

    Lifescience Database Archive (English)

    Full Text Available xpression; interacts with protein kinase Snf1p, glucose sensors Snf3p and Rgt2p, and TATA-binding protein Sp...; interacts with protein kinase Snf1p, glucose sensors Snf3p and Rgt2p, and TATA-binding protein Spt15p; act

  11. Fulltext PDF

    Indian Academy of Sciences (India)

    Arora B M, Tata Institute of Fundamental Research, Mumbai. Awana V P S, National Physical Laboratory, New Delhi. Banerjee S, Bhabha Atomic Research Centre, Mumbai. Bhattacharya S, Tata Institute of Fundamental Research, Mumbai. Chaddah P, UGC-DAE CSR, Indore. Chaplot S L, Bhabha Atomic Research Centre, ...

  12. Proceedings – Mathematical Sciences | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Author Affiliations. Indranil Biswas1 N Raghavendra1 2. School of Mathematics, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400 005, India; Advanced Technology Centre, Tata Consultancy Services, K.L.K. Estate, Fateh Maidan Road, Hyderabad 500 001, India ...

  13. Triazatriangulene as binding group for molecular electronics

    DEFF Research Database (Denmark)

    Wei, Zhongming; Wang, Xintai; Borges, Anders

    2014-01-01

    The triazatriangulene (TATA) ring system was investigated as a binding group for tunnel junctions of molecular wires on gold surfaces. Self-assembled monolayers (SAMs) of TATA platforms with three different lengths of phenylene wires were fabricated, and their electrical conductance was recorded ...... with its high stability and directionality make this binding group very attractive for molecular electronic measurements and devices. (Figure Presented)....

  14. ANALISIS PENGARUH PENATAAN RUANG TERHADAP KINERJA PEMBANGUNAN WILAYAH DI KABUPATEN WAROPEN PAPUA INDONESIA

    OpenAIRE

    Lazarus Ramandey

    2017-01-01

    Berbagai permasalahan penataan ruang di Kabupaten Waropen Papua menunjukkan bahwa Rencana Tata Ruang Wilayah (RTRW ) Kabupaten Waropen Papua yang disusun tahun 2012 belum memiliki kontribusi positif terhadap penyelesaian permasalahan tata ruang. Hal ini kemungkinan disebabkan karena terjadi inkonsistensi dalam penataan ruang. Penelitian ini mencoba untuk melihat konsistensi penataan ruang serta kaitannya dengan kinerja pembangunan wilayah. Metode yang digunakan u...

  15. ACCURACY ORDER OF THE GRAMMATICAL MORPHEMES IN THE ORAL PRODUCTION OF PRESCHOOL PUPILS

    Directory of Open Access Journals (Sweden)

    Jessie Barrot

    2014-01-01

    KEAKURATAN URUTAN MORFEM TATA BAHASA DALAM PRODUKSI LISAN SISWA PRA-SEKOLAH Abstrak: Ada banyak kajian mengenai urutan pemerolehan morfem-morfem tata bahasa, akan tetapi sepengetahuan penulis tidak ada makalah yang telah menggali keakuratan urutan dan pemerolehan morfem tata bahasa diantara anak-anak Filipina. Kajian ini menyelidiki keakuratan urutan dari empat belas morfem tata bahasa Inggris diantara siswa pra-sekolah Filipina. Khususnya, makalah ini berusaha untuk menentukan morfem-morfem tata bahasa yang memiliki tingkat keakuratan tertinggi dan terendah diantara siswa pra-sekolah Filipina. Kajian ini juga berusaha untuk melihat apakah ada hubungan antara urutan pemerolehan morfem tata bahasa para siswa pra-sekolah Filipina dengan urutan morfem tata bahasa dimana Inggris sebagai bahasa pertama dan kedua. Para peserta yang terlibat dalam kajian ini adalah 18 siswa pra-sekolah yang berumur antara tiga sampai lima tahun. Para peserta ini dibagi ke dalam dua kelompok berdasarkan latar belakang bahasa dan geografisnya. Temuan-temuan menunjukkan bahwa kata-kata kerja jamak dan progresif (sedang berlangsung memiliki keakuratan paling tinggi, sementara kata depan dan kata-kata kerja tidak beraturan lampau memiliki keakuratan yang paling rendah. Mengenai hubungan antara urutan pemerolehan, hasilnya mengungkapkan bahwa kajian ini menunjukkan urutan yang berbeda dibanding dengan hasil kajian Dulay dan Burt (1973 serta Brown (1973 melalui koefisien keselaran (coefficient of concordance Kendall dan Korelasi Urutan Ranking (Rank Order Correlation Spearman.   Katakunci: Keakuratan urutan, pemerolehan bahasa kedua, urutan pemerolehan, morfem tata bahasa

  16. 南中国斑节对虾养殖中控制白斑综合症病的理论和策略%Theory and Strategies for Controlling White Spot Syndrome (WSS) of Cultured Penaeus monodon in South China

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    @@ White spot syndrome virus (WSSV) is the causative agent of white spot syndrome (WSS) of cultured penaeid shrimps. WSS breaks out and prevails in cultured penaeid shrimps in many countries and regions of the world, especially in southeast Asia. WSSV is the virus most severe damaging to the cultured penaeid shrimps in the world. At the present time, the control of the outbreaks of WSSV will recover and develop the penaeid shrimp cultures in China and even in the world.

  17. Class switch recombination in selective IgA-deficient subjects

    DEFF Research Database (Denmark)

    Jensen, Lone Hummelshøj; Ryder, L P; Nielsen, L K

    2006-01-01

    -beta in combination, induced IgA production, albeit lower than found in B cells from controls. The B cells from the IgA-deficient subjects were less effective in differentiating into CD138(+) X-box binding protein 1 (XBP-1)(+) plasma cells when stimulated with TGF-beta, IFN-gamma or IL-10. Interestingly, when adding...

  18. Myeloid-Derived Suppressor Cells in Checkpoint Protein Inhibition for Melanoma

    Science.gov (United States)

    2017-09-01

    9 5. Changes/Problems ------------------------------------------------------------------------------- 11 6. Products ...reverse transcription polymerase chain reaction endoplasmic reticulum inositol-requiring enzyme 1 x-box binding protein-1 osteoprotegerin TNF...interests, and values ; and guides them through creating an IDP that includes both research and career goals. A written plan is created, and a

  19. Xbp1s in Pomc neurons connects ER stress with energy balance and glucose homeostasis

    Science.gov (United States)

    The molecular mechanisms underlying neuronal leptin and insulin resistance in obesity and diabetes remain unclear. Here we show that induction ofthe unfolded protein response transcription factor spliced X-box binding protein 1(Xbp1s) in pro-opio-melanocortin (Pomc) neurons alone is sufficient to pr...

  20. Current status of viral diseases in Indian shrimp aquaculture.

    Science.gov (United States)

    Tandel, G M; John, K Riji; Rosalind George, M; Prince Jeyaseelan, M J

    The intensification of aquaculture has been unique in showing the overwhelming changes in global food production in the last 100 years. Presently, it is playing a vital role in the economies of several countries. Conversely, it is also to be noted that the progression of aquaculture has been the foundation of anthropogenic alteration of a gigantic hierarchy and hence not astonishingly, it resulted in spread and emergence of an increasing group of new unknown diseases. In India, Penaeus monodon, black tiger shrimp was previously the foremost-cultivated shrimp species. Subsequently in 2008, the American white leg shrimp Litopenaeus vannamei has effectively replaced it. The change in dominant species has affected disease concerns in India as well as in world shrimp aquaculture. White spot syndrome virus (WSSV) is the most deleterious for both species. Hepatopancreatic parvovirus (HPV), Monodon baculovirus (MBV) and Infectious hypodermal and hematopoietic necrosis virus (IHHNV) are the other significant infectious agents of P. monodon and L. vannamei. An emerging disease of loose shell syndrome (LSS) was already reported from India during late 1998. A more recent disease of L. vannamei in India is monodon slow growth syndrome (MSGS), a component of which seems to be Laem-Singh virus (LSNV). Thus, most of the information in this review relates to new emerging pathogens that threaten the cultivation shrimp industry in India.

  1. Role of women in Indian shrimp farming

    OpenAIRE

    Gopalakrishnan, A.

    1996-01-01

    Women in India are involved in various facets of shrimp (Penaeus monodon; Penaeus indicus) farming, including pond construction, seed collection, collection of feed materials and preparation of feeds, pond maintenance and post-harvest handling. This study indicates that 40% of laborers involved in shrimp farm construction are women. The various roles of women in shrimp farming are also described.

  2. Food selection preference of different ages and sizes of black tiger ...

    African Journals Online (AJOL)

    Yomi

    2012-03-15

    Mar 15, 2012 ... Key words: Aquaculture, natural food, Penaeus monodon, Malaysia. INTRODUCTION ... Many researchers who reported on the diet of Penaeus species in ..... matter, annelida, mollusca and small fish in estuarine water. .... planktonic and benthic communities to fertilizer and feed application in shrimp ...

  3. Genetic diversity of intensive cultured and wild tiger shrimp Penaeus ...

    African Journals Online (AJOL)

    The objective of this study was to assess the genetic diversity of intensive cultured and wild tiger shrimp Penaeus monodon (Fabricius) in Malaysia using six microsatellite markers (CSCUPmo1, CSCUPmo2, CSCUPmo3, CSCUPmo4, CSCUPmo6 and CSCUPmo7). The mean numbers of allele, observed heterozygosis, ...

  4. Impact of converison of mangrove ecosystem for aquaculture purposes

    Digital Repository Service at National Institute of Oceanography (India)

    Untawale, A.G.; Wafar, S.

    ,000 hectare are produced. The species cultivated are mugil cephalus, Chanon-Chanos, Penaeus Monodon, P. indicus, Macrobrachium, etc. About 32.13% of this area is along west coast and the yield rate is about 500-600 kg/ha/yr. Some of the impacts of conversion...

  5. Studies on the water quality and biota of a tidal eutrophic creek ...

    African Journals Online (AJOL)

    ... E.monodon, Navicula gracilis, N. mutica, Ulnariaulna, Nitzschia palea, Pinnularia braunii, P. gibba, Microcystis aeruginosa and Spirulina platensis. Of the 15 mudflat algae Nitzschia palea, Pinnularia braunii, P. gibba and Spirulina platensis were environmentally important species. The population of the benthic fauna was ...

  6. Food selection preference of different ages and sizes of black tiger ...

    African Journals Online (AJOL)

    However, it was found that the food selection preference of P. monodon is dependent on the availability of food items in the pond bottom. The tendency to prefer natural food by shrimps was observed when the food was available. The benthic organisms declined at the end of the culture period indicating that the culture ...

  7. Ghosh, Prof. Anish

    Indian Academy of Sciences (India)

    Brandeis). Date of birth: 25 December 1979. Specialization: Ergodic Theory, Lie Groups, Number Theory Address: School of Mathematics, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400 005, Maharashtra Contact:

  8. Fulltext PDF

    Indian Academy of Sciences (India)

    user

    SINCE 1955, the Computer Section of the Tata Institute of Fundamental ... briefly the main features of the system and engineering design of this digital computer. In ... rigid acceptance test to check their performance under extreme operating ...

  9. Development of a superconducting LINAC booster for the Pelletron ...

    Indian Academy of Sciences (India)

    Department of Nuclear and Atomic Physics, Tata Institute of Fundamental Research, ... is carried out using periodic quadrupole doublet magnets operating at room ... Superconducting cavity resonator; cryogenic distribution system; pelletron ...

  10. Gavai, Prof. Rajiv Vasantrao

    Indian Academy of Sciences (India)

    Specialization: High Energy Physics, Quantum Field Theory, Lattice Field Theories, Quark-Gluon Plasma, and Relativistic Heavy Ion Collisions and Early Universe Address: Senior Professor, Theoretical Physics Department, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400 005, Maharashtra

  11. 79th Annual Meeting | Annual Meetings | Events | Indian Academy of ...

    Indian Academy of Sciences (India)

    View presentation. 12.20. SOURENDU GUPTA, Tata Institute of Fundamental Research, Mumbai ... Demographic dividend or ticking time bomb? View presentation ... Plant genome analysis for accelerated gene discovery. View presentation.

  12. INDeGenIUS, a new method for high-throughput identification of ...

    Indian Academy of Sciences (India)

    Bio-Sciences Division, Innovation Labs, Tata Consultancy Services, 1 Software Units Layout,. Hyderabad 500 ...... symbionts and pathogens in their quest for interaction with ... vaccines and treatment for a number of human pathogens. Thus ...

  13. Journal of Astrophysics and Astronomy | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Author Affiliations. S. Naik1 A. R. Rao1 Sandip K. Chakrabarti2. Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai, 400 005, India. S. N. Bose National Center for Basic Sciences, Salt Lake, Calcutta, 700 091, India.

  14. Laser Plasmas

    Indian Academy of Sciences (India)

    -focusing in a plasma ... Center for Energy Studies, Indian Institute of Technology, New Delhi 110 016, India; Tata Consultancy Services, Gurgaon, India; Ideal Institute of Technology, Ghaziabad, India; Center for Research in Cognitive, ...

  15. Higgs physics at the Large Hadron Collider

    Indian Academy of Sciences (India)

    lation of higher-order corrections to exclusive distributions is very important. ... bounds from the Tevatron, shown in figure 1, may be dependent on the ..... H Baer and X Tata, Weak scale supersymmetry: From superfields to scattering events.

  16. Fulltext PDF

    Indian Academy of Sciences (India)

    Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400 ... in nature, string theory provides a natural setting to understand gauge field theories. ... way, it is often the case that a continuum picture, even qualitative, can be a ...

  17. Percolation systems away from the critical point

    Indian Academy of Sciences (India)

    DEEPAK DHAR. Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400 005, India ... There is more to percolation theory than the critical exponents. Of course, an experi- .... simple qualitative arguments. In the summation ...

  18. People, places, and infrastructure: Lessons from Mumbai, Rio, and ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    2016-12-13

    Dec 13, 2016 ... ... Policy and Governance at the Tata Institute for Social Sciences in Mumbai. ... profiles and case studies, they explored the trajectory of social and spatial ... including shared values and identity, feelings of belonging, civic pa.

  19. Comparison of thermomagnetic history effects in weakly pinned ...

    Indian Academy of Sciences (India)

    1Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400 005, ... systems, where the characteristic internal structures span the entire PE regime. .... financial support in the form of Kanwal Rekhi Career Development Award.

  20. Proceedings – Mathematical Sciences | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Author Affiliations. Saurav Bhaumik1 Vikram Mehta2. School of Mathematics, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400 005, India; Department of Mathematics, Indian Institute of Technology Bombay, Powai, Mumbai 400 076, India ...

  1. Proceedings – Mathematical Sciences | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Author Affiliations. Hassan Azad1 Indranil Biswas2. Department of Mathematics and Statistics, King Fahd University, Saudi Arabia; School of Mathematics, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400 005, India ...

  2. Journal of Biosciences | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    rkaur@cdfd.org.in) Renu Khanna-Chopra, Indian Agricultural Research Institute, New Delhi (renu_wtc@rediffmail.com) Jitendra P Khurana, University of Delhi, New Delhi (khuranaj@genomeindia.org) Ullas Kolthur, Tata Institute of Fundamental ...

  3. Mandal, Prof. Gautam

    Indian Academy of Sciences (India)

    ), FNA. Date of birth: 15 December 1962. Specialization: Quantum Field Theory, Gravity, String Theory Address: Department of Theoretical Physics, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400 005, Maharashtra

  4. The accidental astronomer

    Indian Academy of Sciences (India)

    Lawrence

    Research Centre (BARC), Tata Institute of Fundamental Research ... to make my Ph.D. a stressful, lengthy process, but I persisted and ... An American graduate student, as- ... prospects of getting a job in Bhubaneswar or its vicinity, as as-.

  5. to view fulltext PDF

    Indian Academy of Sciences (India)

    , USA bass@phy.duke.edu. Basu Madhumita, Physics Department, Bangabasi College, Kolkata 700 009. Bearden Ian, Niels Bohr Institute, Blegdamsvej 17, 2100 Kobenhavn, Denmark bearden@nbi.dk. Bhalerao Rajeev, Tata Institute of ...

  6. Associateship | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Home; Fellowship; Associateship. Associate Profile. Period: 1989–1993. Chakrabarti, Dr S K . Date of birth: 15 November 1958. Specialization: Theoretical Astrophysics Address during Associateship: Theoretical Astrophysics Group, Tata Inst. of ...

  7. 5539 Volume 11 No. 7 December 2011 STUDIES ON THERMAL ...

    African Journals Online (AJOL)

    A R VIJAY

    2011-12-07

    Dec 7, 2011 ... Research Scholar, Dept. of Fish Processing Technology, College of Fisheries .... was tightly screwed into the can body with a rubber gasket so as to ..... Ranganna S Handbook of Canning and Aseptic Packaging, Tata McGraw ...

  8. 76th Annual Meeting | Annual Meetings | Events | Indian Academy of ...

    Indian Academy of Sciences (India)

    Structural materials for the future: The case of bulk metallic glasses ... Cell therapy for ocular surface: A successful model of regenerative medicine. 11.45. SHUBHA TOLE, Tata ... Business Meeting of Fellows (Seminar Hall). 17.30 - 18.00.

  9. Odour avoidance learning in the larva of Drosophila melanogaster

    Indian Academy of Sciences (India)

    Prakash

    Recent experiments with larvae have employed appetitive ... Tata Institute of Fundamental Research, National Centre for Biological Sciences, Bangalore 560 065, India ..... psychology: event timing turns punishment to reward; Nature.

  10. Journal of Biosciences | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    2008-12-11

    Dec 11, 2008 ... Author Affiliations. Sukant Khurana1 Mohammed Bin AbuBaker1 Obaid Siddiqi1. Tata Institute of Fundamental Research, National Centre for Biological Sciences, Bangalore 560 065, India ...

  11. Serotonergic neurons of the Drosophila air-puff-stimulated flight circuit

    Indian Academy of Sciences (India)

    2014-07-07

    Jul 7, 2014 ... Tata Institute of Fundamental Research, Bellary Road,. Bangalore, India ... that aid in the study of behaviour by this paradigm have grown, and here we .... Confocal analysis. 576 ..... (movies were recorded at 25 frames per s).

  12. Fulltext PDF

    Indian Academy of Sciences (India)

    Nuclear Physics, Kolkata. The Institute of Mathematical Sciences, Chennai. Tata Institute of Fundamental Research, Mumbai. Advisory Board. Mustansir Barma, Mumbai. Jayanta Kumar Bhattacharjee, Kolkata. Indrani Bose, Kolkata. Bikas K Chakrabarti, Kolkata. Chandan Dasgupta, Bangalore. Deepak Dhar, Mumbai.

  13. Associateship | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Hosur, Prof. R V . Date of birth: 16 May 1953. Specialization: Conformation of Biomolecules, Magnetic Resonance Address during Associateship: Chemical Physics Group, Tata Institute of Fundamental Res., Homi Bhabha Road, Mumbai 400 005.

  14. Sampathkumaran, Prof. Echur Varadadesikan

    Indian Academy of Sciences (India)

    Specialization: Spin-Chain Behaviour, Kondo Lattices, Strong Electron Correlations, Multiferroics, Magnetism, Superconductivity, Geometrical Frustration Address: Distinguished Professor, Department of Condensed Matter Physics and Materials Science, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai ...

  15. New Fellows and Honorary Fellow

    Indian Academy of Sciences (India)

    Specialization: Superconductivity, Magnetism, Low Temperature Scanning, Tunnelling Microscopy/Spectroscopy, High Frequency Electrodynamics of Superconductors Address: Department of Condensed Matter Physics & Materials Science, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400 005, ...

  16. Lie-optics, geometrical phase and nonlinear dynamics of self ...

    Indian Academy of Sciences (India)

    Home; Journals; Pramana – Journal of Physics; Volume 55; Issue 5-6 ... This latter symmetry, however, needs care in its implementation because the ... India; Tata Consultancy Services, Gurgaon, India; Ideal Institute of Technology, Ghaziabad ...

  17. Quark-gluon plasma: Status of heavy ion physics

    Indian Academy of Sciences (India)

    Department of Theoretical Physics, Tata Institute of Fundamental Research, ... such as quark confinement and chiral symmetry breaking, and quantitative details ... attempts have been made, and are being made, to address these issues.

  18. Line bundles and flat connections

    Indian Academy of Sciences (India)

    1School of Mathematics, Tata Institute of Fundamental Research, Homi Bhabha Road, .... sequence for complex analytic bundles, Appendix to Topological Methods ... Society of Japan 15 (1987) (Iwanami Shoten Publishers and Princeton ...

  19. Mallik, Prof. Roop

    Indian Academy of Sciences (India)

    Specialization: Molecular Motors, Phagosome Dynamics, Lipid Droplets, Triglyceride Secretion from Liver Address: Department of Biological Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400 005, Maharashtra Contact: Office: (022) 2278 2702. Residence: (022) 2280 4703. Fax: (022) ...

  20. TRANES analysis of the fluorescence of nile red in organized ...

    Indian Academy of Sciences (India)

    Unknown

    Department of Chemical Sciences, Tata Institute of Fundamental Research, ... Construction of time-resolved emission spectra (TRES) is a useful method 1. If the ..... 1992–2000 Topics in fluorescence spectroscopy (New York: Plenum) vol. 1–5 ...

  1. Journal of Astrophysics and Astronomy | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Author Affiliations. Pankaj Jain1 S. Sarala2. Physics Department, Indian Institute of Technology, Kanpur 208 016, India. National Centre for Radio Astrophysics, Tata Institute of Fundamental Research, Pune University Campus, Post Bag 3, Ganeshkhind, Pune 411 007, India.

  2. Publications | Page 13 | IDRC - International Development Research ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    2016-10-07

    Results 121 - 130 of 6341 ... Exploring non-violent male identities ... In this 2014 profile, researchers with the Tata Institute of Social Sciences ... At an October 7, 2016 project launch, prominent advocates from the Women's Learning Partnership ...

  3. Structural studies and Tc dependence in La2−xDyxCayBa2Cu4+ ...

    Indian Academy of Sciences (India)

    3Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400 005, India. ∗ ... A new series of mixed oxide superconductors with the stoichiometric ... with least square method and they clearly indicate the single phase and ...

  4. Superconducting LINAC booster for the Mumbai pelletron

    Indian Academy of Sciences (India)

    LINAC), to boost the energy of heavy ion beams from the 14UD Pelletron accelerator, at Tata Institute of Fundamental Research, Mumbai. The accelerating structures in the LINAC are quarter wave resonators (QWR) coated with lead which is ...

  5. Mid Year Meetings

    Indian Academy of Sciences (India)

    10.30, M. KRISHNAMURTHY, Tata Institute of Fundamental Research, Mumbai Making a ... Averaging operations on matrices, View PDF file (220 kbytes) ... Food and bio-process engineering - research contributions, View presentation.

  6. Genotype–phenotype relationship of F7 R353Q polymorphism and ...

    Indian Academy of Sciences (India)

    morphism in the F7 gene in 139 Indian families with CAD, comprising of 222 affected subjects, 105 unaffected .... drugs (statins: 65% vs 3%), anti-hypertensive agents (ACE ..... support extended by the Tata Social Welfare Trust, Garry and Mary.

  7. Vegetative propagation of twelve fodder tree species indigenous to ...

    African Journals Online (AJOL)

    Vegetative propagation of twelve fodder tree species indigenous to the Sahel, West Africa. Catherine Ky-Dembele, Jules Bayala, Antoine Kalinganire, Fatoumata Tata Traoré, Bréhima Koné, Alain Olivier ...

  8. Examining the best-fit paradigm for FEM at element level

    Indian Academy of Sciences (India)

    R. Narasimhan (Krishtel eMaging) 1461 1996 Oct 15 13:05:22

    mathematical physics that are not always amenable to analytical methods. ... Invoking the virtual work principle, one can use an approximate ...... Reddy J N 2003 An introduction to the finite element method (New Delhi: Tata McGraw-Hill).

  9. Validation of liquid biopsy: plasma cell-free DNA testing in clinical management of advanced non-small cell lung cancer

    OpenAIRE

    Veldore,Vidya; Choughule,Anuradha; Routhu,Tejaswi; Mandloi,Nitin; Noronha,Vanita; Joshi,Amit; Dutt,Amit; Gupta,Ravi; Vedam,Ramprasad; Prabhash,Kumar

    2018-01-01

    Vidya H Veldore,1,* Anuradha Choughule,2,* Tejaswi Routhu,1 Nitin Mandloi,1 Vanita Noronha,2 Amit Joshi,2 Amit Dutt,3 Ravi Gupta,1 Ramprasad Vedam,1 Kumar Prabhash2 1MedGenome Labs Private Ltd,, Bangalore, India; 2Tata Memorial Centre, Parel, Mumbai, India; 3The Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Center, Kharghar, Navi Mumbai, Maharashtra, India *These authors contributed equally to this work Abstract: Plasma cell-free tumor DNA, or circulating tumo...

  10. Yeast Interacting Proteins Database: YOR047C, YKL038W [Yeast Interacting Proteins Database

    Lifescience Database Archive (English)

    Full Text Available racts with protein kinase Snf1p, glucose sensors Snf3p and Rgt2p, and TATA-binding protein Spt15p; acts as a...Bait description Protein involved in control of glucose-regulated gene expression; interacts with protein kinase Snf1p, glucose senso...rs Snf3p and Rgt2p, and TATA-binding protein Spt15p; acts as a regulator of the tra

  11. Yeast Interacting Proteins Database: YFR049W, YOR047C [Yeast Interacting Proteins Database

    Lifescience Database Archive (English)

    Full Text Available protein kinase Snf1p, glucose sensors Snf3p and Rgt2p, and TATA-binding protein Spt15p; acts as a regulator... (0) YOR047C STD1 Protein involved in control of glucose-regulated gene expression; interacts with protein kinase Snf1p, glucose sens...ors Snf3p and Rgt2p, and TATA-binding protein Spt15p; ac

  12. Rapport fra 2nd Supply Chain Leaders Forum 2013

    DEFF Research Database (Denmark)

    Knak, Henrik; Hald, Kim Sundtoft

    2013-01-01

    Dette års udgave af Supply Chain Leaders Forum diskede op med innovation, inspiration og ny indsigt i outsourcing. Konferencen var arrangeret i et samarbejde mellem Copenhagen Business School og Tata Consultancy Services. (Artiklen er på engelsk).......Dette års udgave af Supply Chain Leaders Forum diskede op med innovation, inspiration og ny indsigt i outsourcing. Konferencen var arrangeret i et samarbejde mellem Copenhagen Business School og Tata Consultancy Services. (Artiklen er på engelsk)....

  13. A Novel Strategy for Controlling the Metastatic Phenotype: Targeting the SNAG Repression Domain in the SNAIL Zinc-Finger Protein

    Science.gov (United States)

    2007-07-01

    Kingdom; anti– pan -cadherin and anti–"-SMA antibodies were from The Journal of Clinical Investigation http://www.jci.org Volume 117 Number 2...23. Lie-Venema, H., et al. 2003. Ets-1 and Ets-2 tran- scription factors are essential for normal coronary and myocardial development in chicken ...transcription. J. Biol. Chem. 273:18881–18890. 38. Weisman-Shomer, P., Cohen, E., and Fry , M. 2002. Distinct domains in the CArG-box binding fac- tor

  14. Self-Organization, Urban Transformation, and Spatial Planning in Greater Jakarta, Indonesia

    Directory of Open Access Journals (Sweden)

    Yovi Dzulhijjah Rahmawati

    2015-09-01

    Full Text Available Penelitian ini bertujuan untuk mengidentifikasi peran sistem perencanaan tata ruang dalam menghadapi proses perorganisasian diri, sebagaimana dibuktikan oleh transformasi perkotaan yang kompleks di wilayah Jakarta. Wilayah Jakarta adalah salah satu wilayah megapolitan di Asia Tenggara yang mengalami proses transformasi yang cepat. Sangat disayangkan bahwa sistem perencanaan tata ruang yang ada sekarang di wilayah Jakarta belum mampu merespon transformasi kota secara non-linier. Kekurangan ini terbukti dari ketidaksinkronan antara dokumen-dokumen perencanaan tata ruang dan perubahan tata guna lahan perkotaan yang diperkuat dengan proses pengorganisasian diri. Perbedaan antara situasi empiris dan dokumen-dokumen perencanaan tata ruang yang ada telah menghasilkan ketidakcocokan antara sistem perencanaan tata ruang dengan sistem tata ruang di wilayah Jakarta. Ketidakcocokan ini terjadi karena sistem perencanaan tata ruang saat ini tidak mempertimbangkan ketidakpastian di masa depan. Situasi ini mengindikasikan adanya ‘fuzziness’ dalam implementasi sistem dan proses perencanaan tata ruang, sementara transformasi perkotaan telah berkembang sedemikian kompleksnya dan membutuhkan respon yang cepat dan tepat. Untuk dapat merespon ketidakcocokan ini, sistem perencanaan tata ruang di wilayah Jakarta harus lebih memperhatikan sistem perkotaan yang berkembang dalam proses yang tidak linear.Kata kunci. Pengorganisasian diri, transformasi perkotaan, ketidaklinieran, sistem perencanaan, Megapolitan Jakarta. This study aimed to identify the role of spatial planning in facing self-organizing processes as evidenced by a complex urban transformation in Greater Jakarta. Greater Jakarta is one of the mega urban-regions in Southeast Asia that are undergoing a rapid urban transformation process. This urban transformation has been developing through a non-linear transition. Unfortunately, the current spatial planning system in Greater Jakarta is not yet adequately

  15. Antibacterial marine bacterium deter luminous vibriosis in shrimp larvae

    OpenAIRE

    Abraham, T.J.

    2004-01-01

    Inhibitory activity of a marine pigmented bacterium - Alteromonas sp. - isolated from Penaeus monodon Fabricius larva against pathogenic and environmental isolates of Vibrio harveyi was studied. All the isolates were inhibited to varying degrees by Alteromonas sp. in vitro. The antibacterial substance produced by the Alteromonas sp. was soluble in organic solvent and closely bound to the external surface of bacterial cells. The antibacterial Alteromonas sp., when allowed to colonize on shrimp...

  16. Phenotypic and molecular typing of Vibrio harveyi isolates and their pathogenicity to tiger shrimp larvae.

    Science.gov (United States)

    Alavandi, S V; Manoranjita, V; Vijayan, K K; Kalaimani, N; Santiago, T C

    2006-11-01

    The objective of the present study was to identify the biotype(s) and molecular type(s) of Vibrio harveyi associated with pathogenicity in tiger shrimp (Penaeus monodon) larvae. Five luminescent and four nonluminescent V. harveyi isolates were subjected to phenotyping and random amplified polymorphic DNA (RAPD) fingerprinting, and pathogenicity testing to P. monodon mysis. Four isolates induced 34-41% mortality of P. monodon mysis when challenged at the rate of 10(6) CFU ml(-1) within 60 h. Sucrose-fermenting biotypes of V. harveyi appeared to be associated with pathogenicity to larval shrimp. Higher temperature and salinity appeared to play a role on the onset of vibriosis and mortality in the challenged larval shrimp. Pathogenic isolates of V. harveyi could be demarcated as revealed by their clustering in the dendrogram constructed based on the RAPD fingerprints. Nonluminescent V. harveyi also appear to be important aetiological agents of vibriosis of shrimp larvae. Sucrose-fermenting biotypes are likely to be pathogenic. High temperature may trigger onset of vibriosis. Biotyping of V. harveyi isolates and looking for traits, such as ability to ferment sucrose may be helpful in identifying the pathogenic forms, and such approach requires to be investigated further with larger number of isolates.

  17. Penataan Artistik Pada Festival “Gunung Kromong Palimanan”

    Directory of Open Access Journals (Sweden)

    Yayat Hadiyat K

    2015-11-01

    Full Text Available Festival “Gunung Kromong Palimanan” merupakan festival seni budaya sebagai wujud aksi sosial budaya dan  pelestarian seni rakyat yang bermuara pada kepedulian lingkungan. Metode yang digunakan adalah metode riset pengabdian masyarakat dengan mengadakan aksi revitalisasi aset budaya, mengkoordinasi generasi muda, mengadakan festival seni budaya. Melalui metode tersebut, Festival seni budaya dapat terwujud dalam mengatasi perubahan sosial budaya masyarakat dengan berpegang pada nilai-nilai budaya yang berlaku dalam lingkungan masyarakat setempat. Konsep artistik Festival Gunung Kromong menggunakan konsep pertunjukan yang disajikan dengan menggunakan tata panggung realis dan tata pentas prosenium. Kegiatan seni pertunjukan adalah konsep pertunjukan seni tradisional kegiatan yang multi disiplin ilmu, mulai dari penata gerak (koregrafer, tata make up, tata suara (seni musik, seni dekorasi tata panggung (lighting, back droop atau backround. Hasil dari riset pengabdian kepada masyarakat ini berupa festival seni budaya yang terdiri dari arak-arakan (pawai budaya dan sajian seni pertunjukan yang dikemas dalam ‘Gunung Kromong Performing Arts’. Kata kunci: Festival, lighting, back droop, backround, Gunung Kromong Performing art.

  18. Reproductive patterns in demersal crustaceans from the upper boundary of the OMZ off north-central Chile

    Science.gov (United States)

    Gallardo, María de los Ángeles; González López, Andrés E.; Ramos, Marcel; Mujica, Armando; Muñoz, Praxedes; Sellanes, Javier; Yannicelli, Beatriz

    2017-06-01

    Pleuroncodes monodon (Crustacea: Munididae) supports one of the main trawling fisheries over the continental shelf off Chile between 25°S and 37°S within the upper boundary of the oxygen minimum zone (OMZ). Although the reproductive cycle of P. monodon has been described, the relationship between this key biological process and the variability of the OMZ has not been comprehensibly addressed neither for P. monodon nor for other OMZ resident species. In this study a set of 14 quasi-monthly oceanographic cruises carried out between June 2010 and November 2011 were conducted over the continental shelf off Coquimbo (30°S) to investigate the temporal variability of: i) dissolved oxygen concentration, temperature and chlorophyll-a at relevant depths ii) the presence and proportion of occurrence of P. monodon ovigerous females and juveniles from benthic trawls; iii) the presence of different stage larvae in the plankton, and iv) similar biological data for other species from the OMZ and shallower depths crustaceans. During summer months oxygen levels and bottom temperature were lower than in winter, while chlorophyll-a concentration was maximum in summer coinciding with an active (but not maximum) upwelling season. P. monodon maximum egg carrying occurred in winter during periods of increased oxygenation. Egg carrying females were never found at depths where oxygen concentration was below 0.5 ml L-1, while over 50% of the autumn and spring cohorts of juveniles occurred at oxygen concentrations below that level. The depth range occupied by ovigerous females was more restricted than the rest of the population and their depth of occurrence followed the variability of the upper OMZ. The larval release period of OMZ resident species extends over late winter and spring, and its main peak precedes that of coastal species (spring) and the spring-summer chlorophyll-a maximum. We propose that for OMZ resident species, brood carrying during warmer and more oxygenated conditions

  19. Berpikir Sistemik Menggunakan Analisis Soft Systems Methodology dalam Manajemen Data dan Information Governance Pemerintah Daerah DIY

    Directory of Open Access Journals (Sweden)

    RADEN MUHAMMAD AGUNG HARIMURTI PURNOMOJATI

    2016-10-01

    Full Text Available Penelitian ini berjudul “Berpikir Sistemik menggunakan analisis Soft Systems Methodology dalam manajemen data dan tata kelola informasi Pemda DIY”. Penelitian ini bertujuan membuat model konseptual manajemen data dan tatakelola informasi Pemda DIY yang terkoneksi. Metode yang digunakan dalam penelitian ini adalah Metode Sistem Lunak. Penelitian ini menggunakan pendekatan kualitatif. Sementara teknik pengumpulan data yang dipakai adalah wawancara, FGD, dan observasi lapangan. Hasil penelitian ini menunjukan manajemen data dan tata kelola informasi di Pemda DIY menunjukkan ciri-ciri tidak sistemik dan ciri-ciri organisasi yang mengalami ketidak mampuan belajar. Untuk itu perlu perubahan cara berpikir lama (old mindset stakeholder ke cara berpikir baru (new mindset dalam manajemen  data dan tata kelola informasi Pemda DIY

  20. Large area, soft crystalline thin films of N,N' ,N'' -trialkyltriazatriangulenium salts with homeotropic alignment of the discotic cores in a lamellar lattice

    DEFF Research Database (Denmark)

    Just Sørensen, Thomas; Hildebrandt, Christoffer B.; Elm, Jonas

    2012-01-01

    techniques confirm that the 15–30 nm thick films maintain the lamellar structure of the bulk crystals, are flat on macroscopic length scales, and are completely ordered relative to the substrate. The triazatriangulenium molecules are electroactive dyes, and in the highly anisotropic polycrystalline thin film......N,N' ,N'' -Trialkyltriazatriangulenium (R-TATA+) tetrafluoroborate (BF4) salts form highly ordered thin films directly when spin-cast onto rotating substrates. The homogeneous and crystalline thin films show macroscopic order over centimetres. The crystal structures of the R-TATA$BF4 salts...... are investigated and compared for the propyl, 3-methylpentyl and octyl derivatives. In all cases the molecules pack in hexagonally ordered bilayers, with segregation of the ionic groups and the alkyl chains. This is a rare packing motif with the rigid discotic TATA+ cores organized co-planarly in sheets separated...