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Sample records for model ip r-10

  1. IP Network Management Model Based on NGOSS

    Institute of Scientific and Technical Information of China (English)

    ZHANG Jin-yu; LI Hong-hui; LIU Feng

    2004-01-01

    This paper addresses a management model for IP network based on Next Generation Operation Support System (NGOSS). It makes the network management on the base of all the operation actions of ISP, It provides QoS to user service through the whole path by providing end-to-end Service Level Agreements (SLA) management through whole path. Based on web and coordination technology, this paper gives an implement architecture of this model.

  2. An attacker-defender model for IP-based networks

    OpenAIRE

    Barkley, Timothy R.

    2008-01-01

    The Internet Protocol (IP) has emerged as the dominant technology for determining how data is routed across the Internet. Because IP flows are defined essentially in terms of origin-destination (O-D) pairs, we represent IP traffic engineering as a multi-commodity flow problem in which each O-D pair is treated as a separate commodity. We account for the diversity in IP routing by modeling opposite extremes of traffic engineering: "naive" traffic engineering where the IP routes data between...

  3. IP Telephony Interconnection Reference Challenges, Models, and Engineering

    CERN Document Server

    Boucadair, Mohamed; Neves, Pedro Miguel; Einarsson, Olafur Pall

    2011-01-01

    Addressing the growth of IP telephony service offerings within the corporate and residential realm, IP Telephony Interconnection Reference: Challenges, Models, and Engineering examines the technical and regulatory issues related to IP telephony interconnection at the large scale. It describes business and interconnection models, reviews emerging architectures such as IMS and TISPAN, identifies commonly-encountered issues, and supplies solutions to technical issues. The authors offer a detailed overview of SPEERMINT activity and proposed architecture, the current work undertaken in i3 Forum, an

  4. TCP-IP Model in Data Communication and Networking

    OpenAIRE

    Pranab Bandhu Nath; Md.Mofiz Uddin

    2015-01-01

    The Internet protocol suite is the computer networking model and set of communications protocols used on the Internet and similar computer networks. It is commonly known as TCP/IP, because it’s most important protocols, the Transmission Control Protocol (TCP) and the Internet Protocol (IP), were the first networking protocols defined in this standard. Often also called the Internet model, it was originally also known as the DoD model, because the development of the networking mode...

  5. MACE: model based analysis of ChIP-exo.

    Science.gov (United States)

    Wang, Liguo; Chen, Junsheng; Wang, Chen; Uusküla-Reimand, Liis; Chen, Kaifu; Medina-Rivera, Alejandra; Young, Edwin J; Zimmermann, Michael T; Yan, Huihuang; Sun, Zhifu; Zhang, Yuji; Wu, Stephen T; Huang, Haojie; Wilson, Michael D; Kocher, Jean-Pierre A; Li, Wei

    2014-11-10

    Understanding the role of a given transcription factor (TF) in regulating gene expression requires precise mapping of its binding sites in the genome. Chromatin immunoprecipitation-exo, an emerging technique using λ exonuclease to digest TF unbound DNA after ChIP, is designed to reveal transcription factor binding site (TFBS) boundaries with near-single nucleotide resolution. Although ChIP-exo promises deeper insights into transcription regulation, no dedicated bioinformatics tool exists to leverage its advantages. Most ChIP-seq and ChIP-chip analytic methods are not tailored for ChIP-exo, and thus cannot take full advantage of high-resolution ChIP-exo data. Here we describe a novel analysis framework, termed MACE (model-based analysis of ChIP-exo) dedicated to ChIP-exo data analysis. The MACE workflow consists of four steps: (i) sequencing data normalization and bias correction; (ii) signal consolidation and noise reduction; (iii) single-nucleotide resolution border peak detection using the Chebyshev Inequality and (iv) border matching using the Gale-Shapley stable matching algorithm. When applied to published human CTCF, yeast Reb1 and our own mouse ONECUT1/HNF6 ChIP-exo data, MACE is able to define TFBSs with high sensitivity, specificity and spatial resolution, as evidenced by multiple criteria including motif enrichment, sequence conservation, direct sequence pileup, nucleosome positioning and open chromatin states. In addition, we show that the fundamental advance of MACE is the identification of two boundaries of a TFBS with high resolution, whereas other methods only report a single location of the same event. The two boundaries help elucidate the in vivo binding structure of a given TF, e.g. whether the TF may bind as dimers or in a complex with other co-factors. © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

  6. Modeling ChIP sequencing in silico with applications.

    Directory of Open Access Journals (Sweden)

    Zhengdong D Zhang

    Full Text Available ChIP sequencing (ChIP-seq is a new method for genomewide mapping of protein binding sites on DNA. It has generated much excitement in functional genomics. To score data and determine adequate sequencing depth, both the genomic background and the binding sites must be properly modeled. To develop a computational foundation to tackle these issues, we first performed a study to characterize the observed statistical nature of this new type of high-throughput data. By linking sequence tags into clusters, we show that there are two components to the distribution of tag counts observed in a number of recent experiments: an initial power-law distribution and a subsequent long right tail. Then we develop in silico ChIP-seq, a computational method to simulate the experimental outcome by placing tags onto the genome according to particular assumed distributions for the actual binding sites and for the background genomic sequence. In contrast to current assumptions, our results show that both the background and the binding sites need to have a markedly nonuniform distribution in order to correctly model the observed ChIP-seq data, with, for instance, the background tag counts modeled by a gamma distribution. On the basis of these results, we extend an existing scoring approach by using a more realistic genomic-background model. This enables us to identify transcription-factor binding sites in ChIP-seq data in a statistically rigorous fashion.

  7. TCP-IP Model in Data Communication and Networking

    Directory of Open Access Journals (Sweden)

    Pranab Bandhu Nath

    2015-10-01

    Full Text Available The Internet protocol suite is the computer networking model and set of communications protocols used on the Internet and similar computer networks. It is commonly known as TCP/IP, because it’s most important protocols, the Transmission Control Protocol (TCP and the Internet Protocol (IP, were the first networking protocols defined in this standard. Often also called the Internet model, it was originally also known as the DoD model, because the development of the networking model was funded by DARPA, an agency of the United States Department of Defense. TCP/IP provides end-to-end connectivity specifying how data should be packetized, addressed, transmitted, routed and received at the destination. This functionality is organized into four abstraction layers which are used to sort all related protocols according to the scope of networking involved. From lowest to highest, the layers are the link layer, containing communication technologies for a single network segment (link; the internet layer, connecting hosts across independent networks, thus establishing internetworking; the transport layer handling host-to-host communication; and the application layer, which provides process-to-process application data exchange. Our aim is describe operation & models of TCP-IP suite in data communication networking

  8. Performance analysis of IP QoS provision model

    Institute of Scientific and Technical Information of China (English)

    SUN Danning; Moonsik Kang

    2006-01-01

    The Performance of a heterogeneous IP QoS provision service model was analyzed. This model utilized RSVP technique to set up dynamic resource reservation interface between the user and the network, meanwhile, DiffServ technique was utilized to transmit class-based packets in different per hop behaviors. Furthermore, accordingly queue management and packets scheduling mechanisms were presented for end-to-end QoS guarantees and appropriate cooperation of network elements.

  9. Service Model for Multi-Provider IP Service Management

    Institute of Scientific and Technical Information of China (English)

    YU Cheng-zhi; SONG Han-tao; LIU Li

    2005-01-01

    In order to solve the problems associated with Internet IP services management, a generic service model for multi-provider IP service management is proposed, which is based on a generalization of the bandwidth broker idea introduced in the differentiated services (DiffServ) environment. This model consists of a hierarchy of service brokers, which makes it fit into providing end-to-end Internet services with QoS support. A simple and scalable mechanism is used to communicate with other cooperative domains to enable customers to dynamically setup services connections over multiple DiffServ domains. The simulation results show that the proposed model is real-time, which can deal with many flow requests in a short period of time, so that it is fit for the service management in a reasonably large network.

  10. Joint modeling of ChIP-seq data via a Markov random field model

    NARCIS (Netherlands)

    Bao, Yanchun; Vinciotti, Veronica; Wit, Ernst; 't Hoen, Peter A C

    2014-01-01

    Chromatin ImmunoPrecipitation-sequencing (ChIP-seq) experiments have now become routine in biology for the detection of protein-binding sites. In this paper, we present a Markov random field model for the joint analysis of multiple ChIP-seq experiments. The proposed model naturally accounts for spat

  11. IP-Sat: Impact-Parameter dependent Saturation model; revised

    CERN Document Server

    Rezaeian, Amir H; Van de Klundert, Merijn; Venugopalan, Raju

    2013-01-01

    In this talk, we present a global analysis of available small-x data on inclusive DIS and exclusive diffractive processes, including the latest data from the combined HERA analysis on reduced cross sections within the Impact-Parameter dependent Saturation (IP-Sat) Model. The impact-parameter dependence of dipole amplitude is crucial in order to have a unified description of both inclusive and exclusive diffractive processes. With the parameters of model fixed via a fit to the high-precision reduced cross-section, we compare model predictions to data for the structure functions, the longitudinal structure function, the charm structure function, exclusive vector mesons production and Deeply Virtual Compton Scattering (DVCS). Excellent agreement is obtained for the processes considered at small x in a wide range of Q^2.

  12. Impact of time-domain IP pulse length on measured data and inverted models

    DEFF Research Database (Denmark)

    Olsson, P. I.; Fiandaca, G.; Dahlin, T.;

    2015-01-01

    The duration of time domain (TD) induced polarization (IP) current injections has significant impact on the acquired IP data as well as on the inversion models, if the standard evaluation procedure is followed. However, it is still possible to retrieve similar inversion models if the waveform...... of the injected current and the IP response waveform are included in the inversion. The on-time also generally affects the signal-tonoise ratio (SNR) where an increased on-time gives higher SNR for the IP data....

  13. The elite and stochastic model for iPS cell generation: multilineage-differentiating stress enduring (Muse) cells are readily reprogrammable into iPS cells.

    Science.gov (United States)

    Wakao, Shohei; Kitada, Masaaki; Dezawa, Mari

    2013-01-01

    Induced pluripotent stem (iPS) cells have attracted a great deal of attention, although the mechanism by which they are generated is still not fully understood. Currently, two theories, the stochastic and elite models, have been proposed. Some reports provide theoretical support for the stochastic model. Other reports, however, support the elite model. For example, some human fibroblasts, such as Multilineage-differentiating stress enduring (Muse) cells, are reported to be pluripotent and a primary source of iPS cells. Thus, the mechanism of iPS cell generation continues to be debated. In this review, we discuss the properties of the original cell source, such as the components of the original populations and the potential of each population to become iPS cells, and further discuss the implications of the two theories for iPS cell research.

  14. Credibility and validation of simulation models for tactical IP networks

    NARCIS (Netherlands)

    Boltjes, B.; Thiele, F.; Diaz, I.F.

    2007-01-01

    The task of TNO is to provide predictions of the scalability and performance of the new all-IP tactical networks of the Royal Netherlands Army (RNLA) that are likely to be fielded. The inherent properties of fielded tactical networks, such as low bandwidth and Quality of Service (QoS) policies

  15. Determination of the Parameter Sets for the Best Performance of IPS-driven ENLIL Model

    Science.gov (United States)

    Yun, Jongyeon; Choi, Kyu-Cheol; Yi, Jonghyuk; Kim, Jaehun; Odstrcil, Dusan

    2016-12-01

    Interplanetary scintillation-driven (IPS-driven) ENLIL model was jointly developed by University of California, San Diego (UCSD) and National Aeronaucics and Space Administration/Goddard Space Flight Center (NASA/GSFC). The model has been in operation by Korean Space Weather Cetner (KSWC) since 2014. IPS-driven ENLIL model has a variety of ambient solar wind parameters and the results of the model depend on the combination of these parameters. We have conducted researches to determine the best combination of parameters to improve the performance of the IPS-driven ENLIL model. The model results with input of 1,440 combinations of parameters are compared with the Advanced Composition Explorer (ACE) observation data. In this way, the top 10 parameter sets showing best performance were determined. Finally, the characteristics of the parameter sets were analyzed and application of the results to IPS-driven ENLIL model was discussed.

  16. Bayesian Modeling of ChIP-chip Data Through a High-Order Ising Model

    KAUST Repository

    Mo, Qianxing

    2010-01-29

    ChIP-chip experiments are procedures that combine chromatin immunoprecipitation (ChIP) and DNA microarray (chip) technology to study a variety of biological problems, including protein-DNA interaction, histone modification, and DNA methylation. The most important feature of ChIP-chip data is that the intensity measurements of probes are spatially correlated because the DNA fragments are hybridized to neighboring probes in the experiments. We propose a simple, but powerful Bayesian hierarchical approach to ChIP-chip data through an Ising model with high-order interactions. The proposed method naturally takes into account the intrinsic spatial structure of the data and can be used to analyze data from multiple platforms with different genomic resolutions. The model parameters are estimated using the Gibbs sampler. The proposed method is illustrated using two publicly available data sets from Affymetrix and Agilent platforms, and compared with three alternative Bayesian methods, namely, Bayesian hierarchical model, hierarchical gamma mixture model, and Tilemap hidden Markov model. The numerical results indicate that the proposed method performs as well as the other three methods for the data from Affymetrix tiling arrays, but significantly outperforms the other three methods for the data from Agilent promoter arrays. In addition, we find that the proposed method has better operating characteristics in terms of sensitivities and false discovery rates under various scenarios. © 2010, The International Biometric Society.

  17. Application of Model Reference Adaptive Control System to Instrument Pointing System /IPS/

    Science.gov (United States)

    Waites, H. B.

    1979-01-01

    A Model Reference Adaptive Controller (MRAC) is derived for a Shuttle payload called the Instrument Pointing System (IPS). The unique features of this MRAC design are that total state feedback is not required, that the internal structure of the model is independent of the internal structure of the IPS, and that the model input is of bounded variation and not required a priori. An application of Liapunov's stability theorems is used to synthesize a control signal which assures MRAC asymptotic stability. Exponential observers are used to obtain the necessary state information to implement the control synthesis. Results are presented which show how effectively the MRAC can maneuver the IPS.

  18. Model Based System Engineering Approach of a Lightweight Embedded TCP/IP

    Directory of Open Access Journals (Sweden)

    M. Z. Rashed

    2011-04-01

    Full Text Available The use of embedded software is growing very rapidly. Accessing the internet is a necessaryservice which has large range of applications in many fields. The Internet is based on TCP/IPwhich is a very important stack. Although TCP/IP is very important there is not a softwareengineering model describing it. The common method in modeling and describing TCP/IP is RFCswhich is not sufficient for software engineer and developers. Therefore there is a need for softwareengineering approach to help engineers and developers to customize their own web basedapplications for embedded systems.This research presents a model based system engineering approach of lightweight TCP/IP. Themodel contains the necessary phases for developing a lightweight TCP/IP for embedded systems.The proposed model is based on SysML as a model based system engineering language.

  19. ChIP-BIT: Bayesian inference of target genes using a novel joint probabilistic model of ChIP-seq profiles.

    Science.gov (United States)

    Chen, Xi; Jung, Jin-Gyoung; Shajahan-Haq, Ayesha N; Clarke, Robert; Shih, Ie-Ming; Wang, Yue; Magnani, Luca; Wang, Tian-Li; Xuan, Jianhua

    2016-04-20

    Chromatin immunoprecipitation with massively parallel DNA sequencing (ChIP-seq) has greatly improved the reliability with which transcription factor binding sites (TFBSs) can be identified from genome-wide profiling studies. Many computational tools are developed to detect binding events or peaks, however the robust detection of weak binding events remains a challenge for current peak calling tools. We have developed a novel Bayesian approach (ChIP-BIT) to reliably detect TFBSs and their target genes by jointly modeling binding signal intensities and binding locations of TFBSs. Specifically, a Gaussian mixture model is used to capture both binding and background signals in sample data. As a unique feature of ChIP-BIT, background signals are modeled by a local Gaussian distribution that is accurately estimated from the input data. Extensive simulation studies showed a significantly improved performance of ChIP-BIT in target gene prediction, particularly for detecting weak binding signals at gene promoter regions. We applied ChIP-BIT to find target genes from NOTCH3 and PBX1 ChIP-seq data acquired from MCF-7 breast cancer cells. TF knockdown experiments have initially validated about 30% of co-regulated target genes identified by ChIP-BIT as being differentially expressed in MCF-7 cells. Functional analysis on these genes further revealed the existence of crosstalk between Notch and Wnt signaling pathways.

  20. IP Models to Orchestrate Innovation Ecosystems: IMEC, A PUBLIC RESEARCH INSTITUTE IN NANO-ELECTRONICS

    OpenAIRE

    Leten, Bart; Vanhaverbeke, Wim; Roijakkers, Nadine; Clerix, André; Van Helleputte, Johan

    2013-01-01

    Companies increasingly organize innovation activities within innovation ecosystems. This study illustrates the central role of the IP-model that an orchestrator develops for the innovation ecosystem partners. The governance of IP is instrumental for the success of innovation ecosystems as it determines the value appropriation potential for the ecosystem partners and positively influences the success of innovation ecosystems. The insights are based on a case study of IMEC, a public research in...

  1. Modeling and performance analysis of IP access interface in optical transmission networks with packet switching

    OpenAIRE

    Lackovic, Marko; Bungarzeanu, Cristian

    2006-01-01

    The article analyzes the influence of the Internet Protocol (IP) access interface on the packet loss probability and delay times in the optical packet switched network. The network and node model have been proposed, and the structure of the IP access interface, including assembler and holder, have been included in the analysis. It has been shown that the increase of the maximum optical packet sizes, as well as use of the holding feature as contention resolution mechanism, decrease the packet ...

  2. IP cores design from specifications to production modeling, verification, optimization, and protection

    CERN Document Server

    Mohamed, Khaled Salah

    2016-01-01

    This book describes the life cycle process of IP cores, from specification to production, including IP modeling, verification, optimization, and protection. Various trade-offs in the design process are discussed, including  those associated with many of the most common memory cores, controller IPs  and system-on-chip (SoC) buses. Readers will also benefit from the author’s practical coverage of new verification methodologies. such as bug localization, UVM, and scan-chain.  A SoC case study is presented to compare traditional verification with the new verification methodologies. ·         Discusses the entire life cycle process of IP cores, from specification to production, including IP modeling, verification, optimization, and protection; ·         Introduce a deep introduction for Verilog for both implementation and verification point of view.  ·         Demonstrates how to use IP in applications such as memory controllers and SoC buses. ·         Describes a new ver...

  3. A kinetic model for type I and II IP3R accounting for mode changes.

    Science.gov (United States)

    Siekmann, Ivo; Wagner, Larry E; Yule, David; Crampin, Edmund J; Sneyd, James

    2012-08-22

    Based upon an extensive single-channel data set, a Markov model for types I and II inositol trisphosphate receptors (IP(3)R) is developed. The model aims to represent accurately the kinetics of both receptor types of IP(3)R depending on the concentrations of inositol trisphosphate (IP(3)), adenosine trisphosphate (ATP), and intracellular calcium (Ca(2+)). In particular, the model takes into account that for some combinations of ligands the IP(3)R switches between extended periods of inactivity alternating with intervals of bursting activity (mode changes). In a first step, the inactive and active modes are modeled separately. It is found that, within modes, both receptor types are ligand-independent. In a second step, the submodels are connected by transition rates. Ligand-dependent regulation of the channel activity is achieved by modulating these transitions between active and inactive modes. As a result, a compact representation of the IP(3)R is obtained that accurately captures stochastic single-channel dynamics including mode changes in a model with six states and 10 rate constants, only two of which are ligand-dependent.

  4. Inflammation and Atrophy Precede Prostate Neoplasia in PhIP Induced Rat Model

    Energy Technology Data Exchange (ETDEWEB)

    Borowsky, A D; Dingley, K; Ubick, E; Turteltaub, K; Cardiff, R D; DeVere-White, R

    2006-06-01

    2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine (PhIP) has been implicated as a major mutagenic heterocyclic amine in the human diet and is carcinogenic in the rat prostate. In order to validate PhIP induced rat prostate neoplasia as a model of human prostate cancer progression, we sought to study the earliest histologic and morphologic changes in the prostate and to follow the progressive changes over time. We fed 67 male Fischer F344 5 week old rats with PhIP (400 PPM) or control diets for 20 weeks, and then sacrificed animals for histomorphologic examination at age 25 weeks, 45 weeks, and 65 weeks. Animals treated with PhIP showed significantly more inflammation (P=.002 (25wk), >.001(45wk), .016(65wk)) and atrophy (P=.003(25wk), >.001(45wk), .006 (65wk)) in their prostate glands relative to controls. Prostatic intraepithelial neoplasia (PIN) occurred only in PhIP treated rats. PIN lesions arose in areas of glandular atrophy, most often in the ventral prostate. Atypical cells in areas of atrophy show loss of glutathione S-transferase pi immunostaining preceding development of PIN. None of the animals in this study developed invasive carcinomas differing from previous reports. Overall, these findings suggest that the pathogenesis of prostatic neoplasia in the PhIP treated rat prostate proceeds from inflammation to post-inflammatory proliferative atrophy to PIN.

  5. Inflammation and Atrophy Precede Prostatic Neoplasia in a PhIP-Induced Rat Model

    Directory of Open Access Journals (Sweden)

    Alexander D. Borowsky

    2006-09-01

    Full Text Available 2-Amino-1-methyl-6-phenylimidazo(4,5-bpyridine (PhIP has been implicated as a major mutagenic heterocyclicamine in the human diet and is carcinogenic in the rat prostate. To validate PhIP-induced rat prostatic neoplasia as a model of human prostate cancer progression, we sought to study the earliest histologic and morphologic changes in the prostate and to follow progressive changes over time. We fed sixty-seven 5-week-old male Fischer F344 rats with PhIP (400 ppm or control diets for 20 weeks, and then sacrificed animals for histomorphologic examination at the ages of 25, 45, and 65 weeks. Animals treated with PhIP showed significantly more inflammation (P = .002, > .001, and .016 for 25, 45, and 65 weeks, respectively and atrophy (P = .003, > .001, and .006 for 25, 45, and 65 weeks, respectively in their prostate glands relative to controls. Prostatic intraepithelial neoplasia (PIN occurred only in PhIP-treated rats. PIN lesions arose in areas of glandular atrophy, most often in the ventral prostate. Atypical cells in areas of atrophy show loss of glutathione S-transferase π immunostaining preceding the development of PIN.None of the animals in this study developed invasive carcinomas, differing from those in previous reports. Overall, these findings suggest that the pathogenesis of prostatic neoplasia in the PhIP-treated rat prostate proceeds from inflammation to postinflammatory proliferative atrophy to PIN.

  6. LegumeIP: an integrative database for comparative genomics and transcriptomics of model legumes.

    Science.gov (United States)

    Li, Jun; Dai, Xinbin; Liu, Tingsong; Zhao, Patrick Xuechun

    2012-01-01

    Legumes play a vital role in maintaining the nitrogen cycle of the biosphere. They conduct symbiotic nitrogen fixation through endosymbiotic relationships with bacteria in root nodules. However, this and other characteristics of legumes, including mycorrhization, compound leaf development and profuse secondary metabolism, are absent in the typical model plant Arabidopsis thaliana. We present LegumeIP (http://plantgrn.noble.org/LegumeIP/), an integrative database for comparative genomics and transcriptomics of model legumes, for studying gene function and genome evolution in legumes. LegumeIP compiles gene and gene family information, syntenic and phylogenetic context and tissue-specific transcriptomic profiles. The database holds the genomic sequences of three model legumes, Medicago truncatula, Glycine max and Lotus japonicus plus two reference plant species, A. thaliana and Populus trichocarpa, with annotations based on UniProt, InterProScan, Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes databases. LegumeIP also contains large-scale microarray and RNA-Seq-based gene expression data. Our new database is capable of systematic synteny analysis across M. truncatula, G. max, L. japonicas and A. thaliana, as well as construction and phylogenetic analysis of gene families across the five hosted species. Finally, LegumeIP provides comprehensive search and visualization tools that enable flexible queries based on gene annotation, gene family, synteny and relative gene expression.

  7. Modeling Alzheimer's disease with human induced pluripotent stem (iPS) cells.

    Science.gov (United States)

    Mungenast, Alison E; Siegert, Sandra; Tsai, Li-Huei

    2016-06-01

    In the last decade, induced pluripotent stem (iPS) cells have revolutionized the utility of human in vitro models of neurological disease. The iPS-derived and differentiated cells allow researchers to study the impact of a distinct cell type in health and disease as well as performing therapeutic drug screens on a human genetic background. In particular, clinical trials for Alzheimer's disease (AD) have been failing. Two of the potential reasons are first, the species gap involved in proceeding from initial discoveries in rodent models to human studies, and second, an unsatisfying patient stratification, meaning subgrouping patients based on the disease severity due to the lack of phenotypic and genetic markers. iPS cells overcome this obstacles and will improve our understanding of disease subtypes in AD. They allow researchers conducting in depth characterization of neural cells from both familial and sporadic AD patients as well as preclinical screens on human cells. In this review, we briefly outline the status quo of iPS cell research in neurological diseases along with the general advantages and pitfalls of these models. We summarize how genome-editing techniques such as CRISPR/Cas9 will allow researchers to reduce the problem of genomic variability inherent to human studies, followed by recent iPS cell studies relevant to AD. We then focus on current techniques for the differentiation of iPS cells into neural cell types that are relevant to AD research. Finally, we discuss how the generation of three-dimensional cell culture systems will be important for understanding AD phenotypes in a complex cellular milieu, and how both two- and three-dimensional iPS cell models can provide platforms for drug discovery and translational studies into the treatment of AD.

  8. GenoGAM: genome-wide generalized additive models for ChIP-Seq analysis.

    Science.gov (United States)

    Stricker, Georg; Engelhardt, Alexander; Schulz, Daniel; Schmid, Matthias; Tresch, Achim; Gagneur, Julien

    2017-08-01

    Chromatin immunoprecipitation followed by deep sequencing (ChIP-Seq) is a widely used approach to study protein-DNA interactions. Often, the quantities of interest are the differential occupancies relative to controls, between genetic backgrounds, treatments, or combinations thereof. Current methods for differential occupancy of ChIP-Seq data rely however on binning or sliding window techniques, for which the choice of the window and bin sizes are subjective. Here, we present GenoGAM (Genome-wide Generalized Additive Model), which brings the well-established and flexible generalized additive models framework to genomic applications using a data parallelism strategy. We model ChIP-Seq read count frequencies as products of smooth functions along chromosomes. Smoothing parameters are objectively estimated from the data by cross-validation, eliminating ad hoc binning and windowing needed by current approaches. GenoGAM provides base-level and region-level significance testing for full factorial designs. Application to a ChIP-Seq dataset in yeast showed increased sensitivity over existing differential occupancy methods while controlling for type I error rate. By analyzing a set of DNA methylation data and illustrating an extension to a peak caller, we further demonstrate the potential of GenoGAM as a generic statistical modeling tool for genome-wide assays. Software is available from Bioconductor: https://www.bioconductor.org/packages/release/bioc/html/GenoGAM.html . gagneur@in.tum.de. Supplementary information is available at Bioinformatics online.

  9. Approaches in highly parameterized inversion - GENIE, a general model-independent TCP/IP run manager

    Science.gov (United States)

    Muffels, Christopher T.; Schreuder, Willem A.; Doherty, John E.; Karanovic, Marinko; Tonkin, Matthew J.; Hunt, Randall J.; Welter, David E.

    2012-01-01

    GENIE is a model-independent suite of programs that can be used to generally distribute, manage, and execute multiple model runs via the TCP/IP infrastructure. The suite consists of a file distribution interface, a run manage, a run executer, and a routine that can be compiled as part of a program and used to exchange model runs with the run manager. Because communication is via a standard protocol (TCP/IP), any computer connected to the Internet can serve in any of the capacities offered by this suite. Model independence is consistent with the existing template and instruction file protocols of the widely used PEST parameter estimation program. This report describes (1) the problem addressed; (2) the approach used by GENIE to queue, distribute, and retrieve model runs; and (3) user instructions, classes, and functions developed. It also includes (4) an example to illustrate the linking of GENIE with Parallel PEST using the interface routine.

  10. PENERAPAN MODEL PEMBELAJARAN INQUIRI TERHADAP HASIL BELAJAR SISWA SEKOLAH DASAR PADA MATA PELAJARAN IPS

    Directory of Open Access Journals (Sweden)

    Damanhuri Damanhuri

    2016-09-01

    Full Text Available Abstrak. Tujuan Penelitian ini adalah untuk meningkatkan hasil belajar siswa dalam pembelajaran IPS materi Proklamsi Kemerdekaan Indonesia menggunakan model pembelajaran inquiri pada siswa kelas V SDN Anyar II Kecamatan Anyar Kabupaten Serang. Hipotesis tindakan dalam penelitian ini adalah mengguankan model pembelajaran Inquiri  dapat meningkatkan hasil belajar siswa pada pembelajaran IPS kelas V SDN Anyar II Kecamatan Anyar Kabupaten Serang Tahun pelajaran 2016/2017. Jenis penelitian yang digunakan adalah penelitian Kuantitatif. Subjek dalam penelitian ini adalah siswa kelas V SDN Anyar II Kecamatan Anyar Kabupaten Serang yang berjumlah 70 siswa yang terdiri 35 siswa kelas A dan 35 kelas B.Data hasil penelitian diperoleh dari pretest, postest, dokumentasi, untuk analisi data kuantitatif tersebut diolah dengan rumus-rumus statistika. Berdasrkan hasil analisis data penelitian dan pengujian hipotesis berdasarkan hasil analisis data penelitian dan pengujian hipotesis Terdapat perbedaan yang signifikan pada mata pelajaran IPS terhadap hasil belajar siswa menggunakan Model Pembelajaran Inquiri, dengan siswa yang menggunakan model pembelajaran langsung. Kata Kunci: Hasil Belajar IPS, Inquiri, Penelitian Kuantitatif  Abstract. The Purpose of this research is to improve to improve the learning outcomes of students in Social Science learning material Indonesia proclamation of independence using a model learning enquiries on grade V SDN Anyer Anyer Sub-District II of the Serang Regency. The hypothesis in this study is the Act of using a Model learning Enquiries can improve student learning outcomes in learning IPS class V SDN Anyer Anyer Sub-District II of the Serang Regency Year 2016/2017 lessons. This type of research is quantitative research. The subject in this study is the grade V SDN Anyer Anyer Sub-District II of Serang district 70 students composed 35 students of class A and class B 35. Data research results obtained from the pretest

  11. Evaluation of IP Portfolios

    DEFF Research Database (Denmark)

    Søberg, Peder Veng

    2009-01-01

    As a result of an inquiry concerning how to evaluate IP (intellectual property) portfolios in order to enable the best possible use of IP resources within organizations, an IP evaluation approach primarily applicable for patents and utility models is developed. The developed approach is useful...

  12. A hidden Ising model for ChIP-chip data analysis

    KAUST Repository

    Mo, Q.

    2010-01-28

    Motivation: Chromatin immunoprecipitation (ChIP) coupled with tiling microarray (chip) experiments have been used in a wide range of biological studies such as identification of transcription factor binding sites and investigation of DNA methylation and histone modification. Hidden Markov models are widely used to model the spatial dependency of ChIP-chip data. However, parameter estimation for these models is typically either heuristic or suboptimal, leading to inconsistencies in their applications. To overcome this limitation and to develop an efficient software, we propose a hidden ferromagnetic Ising model for ChIP-chip data analysis. Results: We have developed a simple, but powerful Bayesian hierarchical model for ChIP-chip data via a hidden Ising model. Metropolis within Gibbs sampling algorithm is used to simulate from the posterior distribution of the model parameters. The proposed model naturally incorporates the spatial dependency of the data, and can be used to analyze data with various genomic resolutions and sample sizes. We illustrate the method using three publicly available datasets and various simulated datasets, and compare it with three closely related methods, namely TileMap HMM, tileHMM and BAC. We find that our method performs as well as TileMap HMM and BAC for the high-resolution data from Affymetrix platform, but significantly outperforms the other three methods for the low-resolution data from Agilent platform. Compared with the BAC method which also involves MCMC simulations, our method is computationally much more efficient. Availability: A software called iChip is freely available at http://www.bioconductor.org/. Contact: moq@mskcc.org. © The Author 2010. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org.

  13. Expected IPS variations due to a disturbance described by a 3-D MHD model

    Science.gov (United States)

    Tappin, S. J.; Dryer, M.; Han, S. M.; Wu, S. T.

    1988-01-01

    The variations of interplanetary scintillation due to a disturbance described by a three-dimensional, time-dependent, MHD model of the interplanetary medium are calculated. The resulting simulated IPS maps are compared with observations of real disturbances and it is found that there is some qualitative agreement. It is concluded that the MHD model with a more realistic choice of input conditions would probably provide a useful description of many interplanetary disturbances.

  14. Bayesian modeling of ChIP-chip data using latent variables.

    KAUST Repository

    Wu, Mingqi

    2009-10-26

    BACKGROUND: The ChIP-chip technology has been used in a wide range of biomedical studies, such as identification of human transcription factor binding sites, investigation of DNA methylation, and investigation of histone modifications in animals and plants. Various methods have been proposed in the literature for analyzing the ChIP-chip data, such as the sliding window methods, the hidden Markov model-based methods, and Bayesian methods. Although, due to the integrated consideration of uncertainty of the models and model parameters, Bayesian methods can potentially work better than the other two classes of methods, the existing Bayesian methods do not perform satisfactorily. They usually require multiple replicates or some extra experimental information to parametrize the model, and long CPU time due to involving of MCMC simulations. RESULTS: In this paper, we propose a Bayesian latent model for the ChIP-chip data. The new model mainly differs from the existing Bayesian models, such as the joint deconvolution model, the hierarchical gamma mixture model, and the Bayesian hierarchical model, in two respects. Firstly, it works on the difference between the averaged treatment and control samples. This enables the use of a simple model for the data, which avoids the probe-specific effect and the sample (control/treatment) effect. As a consequence, this enables an efficient MCMC simulation of the posterior distribution of the model, and also makes the model more robust to the outliers. Secondly, it models the neighboring dependence of probes by introducing a latent indicator vector. A truncated Poisson prior distribution is assumed for the latent indicator variable, with the rationale being justified at length. CONCLUSION: The Bayesian latent method is successfully applied to real and ten simulated datasets, with comparisons with some of the existing Bayesian methods, hidden Markov model methods, and sliding window methods. The numerical results indicate that the

  15. Protocol: Chromatin immunoprecipitation (ChIP methodology to investigate histone modifications in two model diatom species

    Directory of Open Access Journals (Sweden)

    Lin Xin

    2012-12-01

    Full Text Available Abstract In this report we describe a chromatin immunoprecipitation (ChIP protocol for two fully sequenced model diatom species Phaeodactylum tricornutum and Thalassiosira pseudonana. This protocol allows the extraction of satisfactory amounts of chromatin and gives reproducible results. We coupled the ChIP assay with real time quantitative PCR. Our results reveal that the two major histone marks H3K4me2 and H3K9me2 exist in P. tricornutum and T. pseudonana. As in other eukaryotes, H3K4me2 marks active genes whereas H3K9me2 marks transcriptionally inactive transposable elements. Unexpectedly however, T. pseudonana housekeeping genes also show a relative enrichment of H3K9me2. We also discuss optimization of the procedure, including growth conditions, cross linking and sonication. Validation of the protocol provides a set of genes and transposable elements that can be used as controls for studies using ChIP in each diatom species. This protocol can be easily adapted to other diatoms and eukaryotic phytoplankton species for genetic and biochemical studies.

  16. Advanced Models and Algorithms for Self-Similar IP Network Traffic Simulation and Performance Analysis

    Science.gov (United States)

    Radev, Dimitar; Lokshina, Izabella

    2010-11-01

    The paper examines self-similar (or fractal) properties of real communication network traffic data over a wide range of time scales. These self-similar properties are very different from the properties of traditional models based on Poisson and Markov-modulated Poisson processes. Advanced fractal models of sequentional generators and fixed-length sequence generators, and efficient algorithms that are used to simulate self-similar behavior of IP network traffic data are developed and applied. Numerical examples are provided; and simulation results are obtained and analyzed.

  17. Energetic Neutral Atom Imaging with the POLAR CEPPAD/ IPS Instrument : Initial Forward Modeling Results

    Science.gov (United States)

    Henderson, M. G.; Reeves, G. D.; Moore, K. R.; Spence, H. E.; Jorgensen, A. M.; Fennell, J. F.; Blake, J. B.; Roelof, E. C.

    1999-01-01

    Although the primary function of the CEPPAD/IPS instrument on Polar is the measurement of energetic ions in-situ, it has also proven to be a very capable Energetic Neutral Atom (ENA) imager. Raw ENA images are currently being constructed on a routine basis with a temporal resolution of minutes during both active and quiet times. However, while analyses of these images by themselves provide much information on the spatial distribution and dynamics of the energetic ion population in the ring current. detailed modeling is required to extract the actual ion distributions. In this paper. we present the initial results of forward modeling an IPS ENA image obtained during a small geo-magnetic storm on June 9, 1997. The equatorial ion distribution inferred with this technique reproduces the expected large noon/midnight and dawn/dusk asymmetries. The limitations of the model are discussed and a number of modifications to the basic forward modeling technique are proposed which should significantly improve its performance in future studies.

  18. Modeling TCP/IP software implementation performance and its application for software routers

    OpenAIRE

    Lepe Aldama, Oscar Iván

    2002-01-01

    Existen numerosos trabajos que estudian o tratan la realización software de los protocolos de comunicaciones para el acceso a la Internet-TCP/IP. Sin embargo, no conocemos ninguno que modele de forma general y precisa la ejecución de este software.La presente tesis aporta una caracterización detallada de la ejecución de la realización software de los mencionados protocolos sobre un computador personal y bajo un sistema operativo UNIX. Esta caracterización muestra cómo varía el rendimiento del...

  19. In silico modeling of Itk activation kinetics in thymocytes suggests competing positive and negative IP4 mediated feedbacks increase robustness.

    Directory of Open Access Journals (Sweden)

    Sayak Mukherjee

    Full Text Available The inositol-phosphate messenger inositol(1,3,4,5tetrakisphosphate (IP4 is essential for thymocyte positive selection by regulating plasma-membrane association of the protein tyrosine kinase Itk downstream of the T cell receptor (TCR. IP4 can act as a soluble analog of the phosphoinositide 3-kinase (PI3K membrane lipid product phosphatidylinositol(3,4,5trisphosphate (PIP3. PIP3 recruits signaling proteins such as Itk to cellular membranes by binding to PH and other domains. In thymocytes, low-dose IP4 binding to the Itk PH domain surprisingly promoted and high-dose IP4 inhibited PIP3 binding of Itk PH domains. However, the mechanisms that underlie the regulation of membrane recruitment of Itk by IP4 and PIP3 remain unclear. The distinct Itk PH domain ability to oligomerize is consistent with a cooperative-allosteric mode of IP4 action. However, other possibilities cannot be ruled out due to difficulties in quantitatively measuring the interactions between Itk, IP4 and PIP3, and in generating non-oligomerizing Itk PH domain mutants. This has hindered a full mechanistic understanding of how IP4 controls Itk function. By combining experimentally measured kinetics of PLCγ1 phosphorylation by Itk with in silico modeling of multiple Itk signaling circuits and a maximum entropy (MaxEnt based computational approach, we show that those in silico models which are most robust against variations of protein and lipid expression levels and kinetic rates at the single cell level share a cooperative-allosteric mode of Itk regulation by IP4 involving oligomeric Itk PH domains at the plasma membrane. This identifies MaxEnt as an excellent tool for quantifying robustness for complex TCR signaling circuits and provides testable predictions to further elucidate a controversial mechanism of PIP3 signaling.

  20. A Bayesian hidden Markov model for motif discovery through joint modeling of genomic sequence and ChIP-chip data.

    Science.gov (United States)

    Gelfond, Jonathan A L; Gupta, Mayetri; Ibrahim, Joseph G

    2009-12-01

    We propose a unified framework for the analysis of chromatin (Ch) immunoprecipitation (IP) microarray (ChIP-chip) data for detecting transcription factor binding sites (TFBSs) or motifs. ChIP-chip assays are used to focus the genome-wide search for TFBSs by isolating a sample of DNA fragments with TFBSs and applying this sample to a microarray with probes corresponding to tiled segments across the genome. Present analytical methods use a two-step approach: (i) analyze array data to estimate IP-enrichment peaks then (ii) analyze the corresponding sequences independently of intensity information. The proposed model integrates peak finding and motif discovery through a unified Bayesian hidden Markov model (HMM) framework that accommodates the inherent uncertainty in both measurements. A Markov chain Monte Carlo algorithm is formulated for parameter estimation, adapting recursive techniques used for HMMs. In simulations and applications to a yeast RAP1 dataset, the proposed method has favorable TFBS discovery performance compared to currently available two-stage procedures in terms of both sensitivity and specificity.

  1. Model Pembelajaran CTL (Contextual Teaching and Learning dalam Meningkatkan Hasil Belajar Mahasiswa PGSD Pada MataKuliah Konsep IPS Dasar

    Directory of Open Access Journals (Sweden)

    Wahyu Susiloningsih

    2016-03-01

    Full Text Available Hasil belajar adalah suatu proses perubahan tingkah laku dalam pengetahuan, sikap, dan ketrampilan yang diperoleh dalam jangka waktu yang lama.Mata kuliah Konsep IPS Dasar merupakan mata kuliah yang memberikan pemahaman kepada mahasiswa PGSD tentang konsep dasar IPS sebagai landasan kajian yang bahannya bersumber dari kehidupan manusia di masyarakat, yang aspek-aspeknya meliputi social science (ilmu sosial, social studies (studi sosial, Ilmu Pengetahuan Sosial (IPS. IPS merupakan pembelajaran pada tingkat sekolah yang berperan mengfungsionalkan ilmu-ilmu sosial yang bersifat teoritik dalam kehidupan nyata dalam masyarakat. Model pembelajaran CTL adalah model pembelajaran yang menuntut kreatifitas guru dalam mengaitkan subject matter dengan kehidupan nyata mahasiswa guna membantu mahasiswa untuk lebih mudah memaknai materi tersebut.

  2. IPS - a System for Real-Time Navigation and 3d Modeling

    Science.gov (United States)

    Grießbach, D.; Baumbach, D.; Börner, A.; Buder, M.; Ernst, I.; Funk, E.; Wohlfeil, J.; Zuev, S.

    2012-07-01

    fdaReliable navigation and 3D modeling is a necessary requirement for any autonomous system in real world scenarios. German Aerospace Center (DLR) developed a system providing precise information about local position and orientation of a mobile platform as well as three-dimensional information about its environment in real-time. This system, called Integral Positioning System (IPS) can be applied for indoor environments and outdoor environments. To achieve high precision, reliability, integrity and availability a multi-sensor approach was chosen. The important role of sensor data synchronization, system calibration and spatial referencing is emphasized because the data from several sensors has to be fused using a Kalman filter. A hardware operating system (HW-OS) is presented, that facilitates the low-level integration of different interfaces. The benefit of this approach is an increased precision of synchronization at the expense of additional engineering costs. It will be shown that the additional effort is leveraged by the new design concept since the HW-OS methodology allows a proven, flexible and fast design process, a high re-usability of common components and consequently a higher reliability within the low-level sensor fusion. Another main focus of the paper is on IPS software. The DLR developed, implemented and tested a flexible and extensible software concept for data grabbing, efficient data handling, data preprocessing (e.g. image rectification) being essential for thematic data processing. Standard outputs of IPS are a trajectory of the moving platform and a high density 3D point cloud of the current environment. This information is provided in real-time. Based on these results, information processing on more abstract levels can be executed.

  3. Hidden Markov Model-based Packet Loss Concealment for Voice over IP

    DEFF Research Database (Denmark)

    Rødbro, Christoffer A.; Murthi, Manohar N.; Andersen, Søren Vang

    2006-01-01

    As voice over IP proliferates, packet loss concealment (PLC) at the receiver has emerged as an important factor in determining voice quality of service. Through the use of heuristic variations of signal and parameter repetition and overlap-add interpolation to handle packet loss, conventional PLC...... systems largely ignore the dynamics of the statistical evolution of the speech signal, possibly leading to perceptually annoying artifacts. To address this problem, we propose the use of hidden Markov models for PLC. With a hidden Markov model (HMM) tracking the evolution of speech signal parameters, we...... demonstrate how PLC is performed within a statistical signal processing framework. Moreover, we show how the HMM is used to index a specially designed PLC module for the particular signal context, leading to signal-contingent PLC. Simulation examples, objective tests, and subjective listening tests...

  4. PENGEMBANGAN MODEL KOLABORASI JIGSAW ROLE PLAYING SEBAGAI UPAYA PENINGKATAN KEMAMPUAN BEKERJASAMA SISWA KELAS V SD PADA PELAJARAN IPS

    Directory of Open Access Journals (Sweden)

    Ika Ari Pratiwi

    2015-11-01

    Full Text Available Penelitian bertujuan untuk mengembangkan model kolaborasi jigsaw, role playing untuk meningkatkan kemampuan bekerjasama siswa yang valid, efektif dan praktis. Metode penelitian adalah penelitian dan pengembangan (R&D. Tahap uji coba pengembangan terdiri atas uji coba ahli, uji coba skala terbatas dan uji coba skala luas. Keefektifan model kolaborasi jigsaw role playing  diperoleh rata-rata 51,83 dalam kategori baik diterapkan dalam pelajaran IPS, peningkatan kemampuan bekerjasama siswa hasil N-gain = 0,56 dengan kategori sedang, peningkatan hasil belajar IPS N-gain = 0,50 dengan kategori sedang dan hasil ketuntasan klasikal pembelajaran IPS 97,14%.  Hasil respon guru dan siswa terhadap model yang digunakan adalah berkriteria baik. Model final penelitian ini menghasilkan model kolaborasi jigsaw role playing yang dikemas dalam suatu buku pedoman.

  5. PENINGKATAN HASIL DAN AKTIVITAS BELAJAR IPS MODEL PROBLEM-BASED LEARNING BERBANTUAN MEDIA SMPN 2 KAWUNGANTEN

    Directory of Open Access Journals (Sweden)

    Suharto Suharto

    2016-12-01

    Full Text Available Penelitian ini bertujuan untuk (1 meningkatkan hasil dan (2 Aktivitas belajar IPS siswa kelas VIII C SMP Negeri 2 Kawunganten Cilacap. Penelitian ini merupakan penelitian tindakan kelas (PTK yang terdiri atas empat pertemuan dalam dua siklus, menggunakan desain Kemmis & Taggart. Subjek penelitian adalah 35 siswa kelas VIII C SMP Negeri 2 Kawunganten Cilacap. Teknik pengumpulan data yang digunakan adalah observasi, tes hasil belajar, angket, dokumentasi, dan catatan lapangan. Teknik analisis data menggunakan analisis deskriptif kuantitatif. Hasil penelitian adalah sebagai berikut: (1 Melalui model pembelajaran Problem-based learning berbantuan media dalam pembelajaran IPS dapat meningkatkan hasil belajar. (2 Ada peningkatan ketuntasan hasil belajar ranah kognitif. Pada siklus I ketuntasan belajar siswa 60,00%, dan siklus II menjadi 85,71%. (3 Ada peningkatan nilai sikap siswa (hasil belajar ranah afektif. Rata-rata nilai sikap siswa 62,20 pada siklus I menjadi 67,97 pada siklus II atau meningkat 5,77 poin. (4 Ada peningkatan aktivitas belajar siswa; siklus I rata-rata aktivitas belajar siswa 3,01 menjadi 3,37 pada siklus II, atau meningkat 0,36 poin.   IMPROVING LEARNING OUTCOME AND ACTIVITIES IN SOCIAL STUDIES OF THE MEDIA-AIDED PROBLEM- BASED LEARNING INSTRUCTIONAL MODEL SMPN 2 KAWUNGANTEN Abstract This study aims to: (1 improve the learning outcome and (2 activities of class VIII C, SMP Negeri 2 Kawunganten Cilacap. This study is classroom action research (CAR consisting of two cycles, each of which consisted of two meetings, using Kemmis & Taggart design. The subject is 35 students of class VIII C, SMP Negeri 2 Kawunganten Cilacap. The data collection techniques used were observation, achievement test, questionnaire, documentation, and field note. The  data analysis used the quantitative descriptive analysis. The results are as follows (1 The through of media-aided Problem-Based Learning instructional model in social studies teaching can

  6. Voice over IP Security

    CERN Document Server

    Keromytis, Angelos D

    2011-01-01

    Voice over IP (VoIP) and Internet Multimedia Subsystem technologies (IMS) are rapidly being adopted by consumers, enterprises, governments and militaries. These technologies offer higher flexibility and more features than traditional telephony (PSTN) infrastructures, as well as the potential for lower cost through equipment consolidation and, for the consumer market, new business models. However, VoIP systems also represent a higher complexity in terms of architecture, protocols and implementation, with a corresponding increase in the potential for misuse. In this book, the authors examine the

  7. Mobile IP

    NARCIS (Netherlands)

    Heijenk, Geert; Sallent, S.; Pras, A.

    1999-01-01

    The Internet is growing exponentially, both in the amount of traffic carried, and in the amount of hosts connected. IP technology is becoming more and more important, in company networks (Intranets), and also in the core networks for the next generation mobile networks. Further, wireless access to I

  8. Mobile IP

    NARCIS (Netherlands)

    Heijenk, Gerhard J.; Sallent, S.; Pras, Aiko

    1999-01-01

    The Internet is growing exponentially, both in the amount of traffic carried, and in the amount of hosts connected. IP technology is becoming more and more important, in company networks (Intranets), and also in the core networks for the next generation mobile networks. Further, wireless access to

  9. Analisa Karakteristik Teori Antrian pada Jaringan IP Multimedia Subsystem (IMS Menggunakan OPNET Modeler 14.5

    Directory of Open Access Journals (Sweden)

    Eko Fajar Cahyadi

    2015-05-01

    Full Text Available Perkembangan teknologi telekomunikasi yang semakin pesat, membuat para operator dan vendor telekomunikasi harus dapat menyediakan layanan telekomunikasi yang aman, cepat, dan multi guna, untuk mendorong terciptanya ekosistem layanan telekomunikasi yang beragam bagi para penggunanya. Next Generation Network (NGN merupakan sebuah teknologi masa depan yang mendukung beberapa layanan terintegrasi seperti voice, data, dan multimedia. Hal utama dari konsep NGN adalah konvergensi dan layanan yang berbasis IP sehingga diperlukan sebuah teknologi yang dikenal dengan IP Multimedia Subsystem (IMS. Agar pengiriman data dalam layanan IMS menjadi lebih efektif dan efisien maka diperlukan sebuah teori antrian yang tepat untuk digunakan sebagai pengatur aliran paket data. Penelitian ini melakukan pengamatan terhadap beberapa layanan pada jaringan IMS dengan menggunakan tiga teori antrian yang berbeda yaitu First In First Out (FIFO, Priority Queuing (PQ, dan Weighted-Fair Queuing (WFQ. Pengamatan dilakukan terhadap layanan Video Conference dan VoIP. Dari hasil simulasi dapat diketahui bahwa teori antrian WFQ pada layanan video conference menghasilkan rata-rata delay sebesar 9.22 ms , delay variation sebesar 0.036 ms dan packet loss sebesar 0.88%. Sedangkan untuk layanan VoIP menghasilkan rata-rata delay sebesar 144 ms, delay variation sebesar 0.021 ms, dan packet loss sebesar 0.05% serta menghasilkan rata-rata throughput sebesar 7.052 Mbps, sehingga teori antrian yang tepat digunakan dalam jaringan IMS dalam penelitian ini adalah WFQ.

  10. Integrative analysis of histone ChIP-seq and transcription data using Bayesian mixture models

    DEFF Research Database (Denmark)

    Klein, Hans-Ulrich; Schäfer, Martin; Porse, Bo T;

    2014-01-01

    Histone modifications are a key epigenetic mechanism to activate or repress the transcription of genes. Datasets of matched transcription data and histone modification data obtained by ChIP-seq exist, but methods for integrative analysis of both data types are still rare. Here, we present a novel...

  11. Testing Brain Overgrowth and Synaptic Models of Autism Using NPC’s and Neurons from Patient-Derived IPS Cells

    Science.gov (United States)

    2014-10-01

    interaction, and limited and repetitive interests and behavior. Recent studies have led to two major hypotheses for autism pathogenesis : early brain...interests and behavior. Recent studies have led to two major hypotheses for autism pathogenesis . First, early brain overgrowth appears to be a critical...Models of Autism Using NPC’s and Neurons from Patient- Derived IPS Cells PRINCIPAL INVESTIGATOR: Fred H. Gage, Ph.D

  12. Running TCP/IP over ATM Networks.

    Science.gov (United States)

    Witt, Michael

    1995-01-01

    Discusses Internet protocol (IP) and subnets and describes how IP may operate over asynchronous transfer mode (ATM). Topics include TCP (transmission control protocol), ATM cells and adaptation layers, a basic architectural model for IP over ATM, address resolution, mapping IP to a subnet technology, and connection management strategy. (LRW)

  13. La gestion des reseaux IP basee sur les technologies Web et le modele push

    OpenAIRE

    1999-01-01

    La gestion des rseaux IP est actuellement fonde sur le protocole SNMP, ainsi que sur des plateformes de gestion coteuses implmentant le paradigme gestionnaire-agent du cadre de gestion SNMP. Cette gestion repose sur deux types de transfert de donnes: un protocole de type requte/rponse, pour recueillir des donnes statistiques et pour surveiller le rseau, et du push non sollicit, pour envoyer les notifications. Ce modle conceptuel souffre de plusieurs carences, notamment en ce qui concerne le t...

  14. miR-10 in development and cancer

    DEFF Research Database (Denmark)

    Lund, Anders Henrik

    2010-01-01

    The microRNA (miRNA) miR-10 family has attracted attention because of its conservation and the position of the miR-10 genes within the Hox clusters of developmental regulators. In several species, miR-10 is coexpressed with a set of Hox genes and has been found to regulate the translation of Hox ...... function to the miRNA repertoire.Cell Death and Differentiation advance online publication, 22 May 2009; doi:10.1038/cdd.2009.58....

  15. Modulation of elementary calcium release mediates a transition from puffs to waves in an IP3R cluster model.

    Directory of Open Access Journals (Sweden)

    Martin Rückl

    2015-01-01

    Full Text Available The oscillating concentration of intracellular calcium is one of the most important examples for collective dynamics in cell biology. Localized releases of calcium through clusters of inositol 1,4,5-trisphosphate receptor channels constitute elementary signals called calcium puffs. Coupling by diffusing calcium leads to global releases and waves, but the exact mechanism of inter-cluster coupling and triggering of waves is unknown. To elucidate the relation of puffs and waves, we here model a cluster of IP3R channels using a gating scheme with variable non-equilibrium IP3 binding. Hybrid stochastic and deterministic simulations show that puffs are not stereotyped events of constant duration but are sensitive to stimulation strength and residual calcium. For increasing IP3 concentration, the release events become modulated at a timescale of minutes, with repetitive wave-like releases interspersed with several puffs. This modulation is consistent with experimental observations we present, including refractoriness and increase of puff frequency during the inter-wave interval. Our results suggest that waves are established by a random but time-modulated appearance of sustained release events, which have a high potential to trigger and synchronize activity throughout the cell.

  16. Business innovation symposium ‘At what price? IP-related thoughts on new business models for space information’

    Science.gov (United States)

    Smith, Lesley Jane

    2011-09-01

    Spatial data and imagery generators are set to become tomorrow's key players in the information society. This is why satellite owners and operators are examining new revenue-producing models for developing space-related products and services. The use and availability of broadband internet width and satellite data-based services will continue to increase in the future. With the capacity to deliver real time precision downstream data, space agencies and the satellite industry can respond to the demand for high resolution digital space information which, with the appropriate technology, can be integrated into a variety of web-based applications. At a time when the traditional roles of space agencies are becoming more hybrid, largely as a result of the greater drive towards commercial markets, new value-added markets for space-related information products are continuing to attract attention. This paper discusses whether traditional data policies on space data access and IP licensing schemes stand to remain the feasible prototype for distributing and marketing space data, and how this growth market might benefit from looking at an 'up and running' global IP management system already operating to manage end user digital demand. PrefaceThe terminology describing the various types of spatial data and space-based information is not uniformly used within the various principles, laws and policies that govern space data. For convenience only this paper refers to primary or raw data gathered by the space-based industry as spatial or raw data, and the data as processed and sold on or distributed by ground-based companies as space information products and services. In practise, spatial data range from generic to specific data sets, digital topography, through to pictures and imagery services at various resolutions, with 3-D perspectives underway. The paper addresses general IP considerations relating to spatial data, with some reference to remote sensing itself. Exact IP details

  17. Modeling the downward transport of {sup 210}Pb in Peatlands: Initial Penetration‐Constant Rate of Supply (IP-CRS) model

    Energy Technology Data Exchange (ETDEWEB)

    Olid, Carolina, E-mail: olid.carolina@gmail.com [Department of Ecology and Environmental Science, Umeå University, SE-90187, Umeå (Sweden); Diego, David [Department of Earth Science, University of Bergen, NO-5020 Bergen (Norway); Garcia-Orellana, Jordi [Departament de Física, Universitat Autònoma de Barcelona, E-08193 Bellaterra (Spain); Institut de Ciència i Tecnologia Ambientals (ICTA), Universitat Autònoma de Barcelona, E-08193 Bellaterra (Spain); Cortizas, Antonio Martínez [Departamento de Edafoloxía e Química Agrícola, Universidade de Santiago de Compostela, E-15782 Santiago de Compostela (Spain); Klaminder, Jonatan [Department of Ecology and Environmental Science, Umeå University, SE-90187, Umeå (Sweden)

    2016-01-15

    The vertical distribution of {sup 210}Pb is commonly used to date peat deposits accumulated over the last 100–150 years. However, several studies have questioned this method because of an apparent post-depositional mobility of {sup 210}Pb within some peat profiles. In this study, we introduce the Initial Penetration–Constant Rate of Supply (IP-CRS) model for calculating ages derived from {sup 210}Pb profiles that are altered by an initial migration of the radionuclide. This new, two-phased, model describes the distribution of atmospheric-derived {sup 210}Pb ({sup 210}Pb{sub xs}) in peat taking into account both incorporation of {sup 210}Pb into the accumulating peat matrix as well as an initial flushing of {sup 210}Pb through the uppermost peat layers. The validity of the IP-CRS model is tested in four anomalous {sup 210}Pb peat records that showed some deviations from the typical exponential decay profile not explained by variations in peat accumulation rates. Unlike the most commonly used {sup 210}Pb-dating model (Constant Rate of Supply (CRS)), the IP-CRS model estimates peat accumulation rates consistent with typical growth rates for peatlands from the same areas. Confidence in the IP-CRS chronology is also provided by the good agreement with independent chronological markers (i.e. {sup 241}Am and {sup 137}Cs). Our results showed that the IP-CRS can provide chronologies from peat records where {sup 210}Pb mobility is evident, being a valuable tool for studies reconstructing past environmental changes using peat archives during the Anthropocene. - Highlights: • Accurate age dating of peat and sediment cores is critical for evaluating change. • A new {sup 210}Pb dating model that includes vertical transport of {sup 210}Pb was developed. • The IP-CRS model provided consistent peat accumulation rates. • The IP-CRS ages were consistent with independent chronological markers. • The IP-CRS model derives peat ages where downward {sup 210}Pb transport is

  18. Foundations of hardware IP protection

    CERN Document Server

    Torres, Lionel

    2017-01-01

    This book provides a comprehensive and up-to-date guide to the design of security-hardened, hardware intellectual property (IP). Readers will learn how IP can be threatened, as well as protected, by using means such as hardware obfuscation/camouflaging, watermarking, fingerprinting (PUF), functional locking, remote activation, hidden transmission of data, hardware Trojan detection, protection against hardware Trojan, use of secure element, ultra-lightweight cryptography, and digital rights management. This book serves as a single-source reference to design space exploration of hardware security and IP protection. · Provides readers with a comprehensive overview of hardware intellectual property (IP) security, describing threat models and presenting means of protection, from integrated circuit layout to digital rights management of IP; · Enables readers to transpose techniques fundamental to digital rights management (DRM) to the realm of hardware IP security; · Introduce designers to the concept of salutar...

  19. The miR-10 microRNA precursor family

    DEFF Research Database (Denmark)

    Tehler, Disa; Høyland-Kroghsbo, Nina Molin; Lund, Anders H

    2011-01-01

    The miR-10 microRNA precursor family encodes a group of short non-coding RNAs involved in gene regulation. The miR-10 family is highly conserved and has sparked the interest of many research groups because of the genomic localization in the vicinity of, coexpression with and regulation of the Hox...... gene developmental regulators. Here, we review the current knowledge of the evolution, physiological function and involvement in cancer of this family of microRNAs....

  20. An integrated model of multiple-condition ChIP-Seq data reveals predeterminants of Cdx2 binding.

    Directory of Open Access Journals (Sweden)

    Shaun Mahony

    2014-03-01

    Full Text Available Regulatory proteins can bind to different sets of genomic targets in various cell types or conditions. To reliably characterize such condition-specific regulatory binding we introduce MultiGPS, an integrated machine learning approach for the analysis of multiple related ChIP-seq experiments. MultiGPS is based on a generalized Expectation Maximization framework that shares information across multiple experiments for binding event discovery. We demonstrate that our framework enables the simultaneous modeling of sparse condition-specific binding changes, sequence dependence, and replicate-specific noise sources. MultiGPS encourages consistency in reported binding event locations across multiple-condition ChIP-seq datasets and provides accurate estimation of ChIP enrichment levels at each event. MultiGPS's multi-experiment modeling approach thus provides a reliable platform for detecting differential binding enrichment across experimental conditions. We demonstrate the advantages of MultiGPS with an analysis of Cdx2 binding in three distinct developmental contexts. By accurately characterizing condition-specific Cdx2 binding, MultiGPS enables novel insight into the mechanistic basis of Cdx2 site selectivity. Specifically, the condition-specific Cdx2 sites characterized by MultiGPS are highly associated with pre-existing genomic context, suggesting that such sites are pre-determined by cell-specific regulatory architecture. However, MultiGPS-defined condition-independent sites are not predicted by pre-existing regulatory signals, suggesting that Cdx2 can bind to a subset of locations regardless of genomic environment. A summary of this paper appears in the proceedings of the RECOMB 2014 conference, April 2-5.

  1. DNA adducts induced by food mutagen PhIP in a mouse model expressing human sulfotransferases 1A1 and 1A2.

    Science.gov (United States)

    Høie, Anja Hortemo; Monien, Bernhard Hans; Glatt, Hansruedi; Hjertholm, Hege; Husøy, Trine

    2016-04-25

    Food processing contaminant 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) has previously been shown to induce formation of DNA adducts in vivo. In a previous study the adduct levels were found to increase in a mouse model expressing human (h) sulfotransferases (SULTs) 1A1 and 1A2 after PhIP exposure, detected by (32)P-postlabelling. Isotope dilution ultra performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS) is emerging as the method of choice for selective and reproducible detection of known DNA adducts. In the present study we investigated the level and distribution of PhIP induced DNA adducts in male FVB mice 9-11 weeks of age with hSULT mice or wild-type mice (wt) using UPLC-MS/MS. Mice received a single administration of 75 mg/kg bw PhIP by oral gavage, and DNA was analysed 3h after exposure. C8-(2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine- N(2)-yl)-2'-deoxyguanosine (C8-PhIP-dG) adduct levels are significantly higher in PhIP exposed hSULT mice compared with PhIP exposed wt mice. The liver was the least affected organ in wild-type mice, whereas it was the most affected organ in hSULT mice with a 14-fold higher adduct level.

  2. Dbp5, Gle1-IP6 and Nup159: a working model for mRNP export.

    Science.gov (United States)

    Folkmann, Andrew W; Noble, Kristen N; Cole, Charles N; Wente, Susan R

    2011-01-01

    Gene expression is a stepwise process involving distinct cellular processes including transcription, mRNA (mRNA) processing, mRNA export, and translation. As mRNAs are being synthesized, proteins associate with the RNA to form messenger ribonucleoprotein particles (mRNPs). Previous studies have demonstrated that the RNA-binding protein composition of these mRNPs is dynamic, changing as the mRNP moves through the different steps of gene expression, and playing a critical role in these events. An important step during this maturation process occurs at the cytoplasmic face of the nuclear pore complex (NPC) where the export protein Gle1 bound to inositol hexakisphosphate (IP 6) spatially activates the ATP-hydrolysis and mRNP-remodeling activity of the DEAD-box protein Dbp5. Recent work from our laboratory and others has provided important insights into the function and regulation of Dbp5. These include a more detailed explanation of the mechanism of Dbp5 RNP remodeling, the role of Gle1-IP6 in stimulating Dbp5 ATPase activity, and the identification of a novel paradigm for regulation of Dbp5 by Nup159. Based on in vitro biochemical assays, X-ray crystallography, and corresponding in vivo phenotypes, we propose here an updated model of the Dbp5 cycle during mRNP export through the NPC. This takes into account all available data and provides a platform for future studies.

  3. Imprvoing QoS of all-IP Generation of Pre-WiMax Networks Using Delay-Jitter Model

    CERN Document Server

    Dahmouni, H; Bonacci, D; Sanso, B; Girard, A

    2010-01-01

    The topic of this paper is the evaluation of QoS parameters in live Pre-Wimax environments. The main contribution is the validation of an analytical delay-jitter behavior model. These models can be used in optimization algorithms in order to provide opportunistic and reliable all-IP networks. It allows understanding the impact of the jitter constraints on the throughput and packet loss in wireless systems. However, we show that the real-time QoS requirements of real-time and interactive services can be avoided to a large degree by controlling only the packet delay-jitter in a fixed and mobile environment. The QoS metrics have been computed from live measurements in a Pre-Wimax realistic environment (Toulouse/Blagnac Airport).

  4. 基于 IP 的集中式会议系统设计与实现%Centralized Conference System Model Based on IP and Its Realization

    Institute of Scientific and Technical Information of China (English)

    马彦彬; 王南洋

    2014-01-01

    This paper identifies the requirements for conference control and presents a centralized conference system model on the base of some scholar research works. The model employs SIP as singling control protocol and comprises some components such as conference configuration, user management, media control, application session management and net-work management. To implement conference control, message mechanism should be employed. This paper adopts a popular solution in which XMLRPC is used as conference command and SIP event framework as conference notification. The mod-el proposed in this paper has been successfully applied to a SIP-based real-time multimedia conference system. The confe-rence control results demonstrate that this model is applicable and effective.%在前人研究的基础上提出了一种独立于应用的、适用于集中式会议的会议控制模型。系统实现的集中的会议管理平台,可以有效的管理各种会议资源,方便各种软硬件媒体资源的接入。采用了 SIP 协议作为会话控制协议。会议控制消息分为命令和通知两种。会议命令使用 XMLRPC 协议实现,会议消息采用了 SIP 事件通知机制。该会议控制模型被成功地应用于基于 SIP 的实时多媒体视频会议系统。实践表明,该会议控制模型是合理的、有效的。

  5. Modelling of pressurised hybrid systems based on integrated planar solid oxide fuel cell (IP-SOFC) technology

    Energy Technology Data Exchange (ETDEWEB)

    Magistri, L.; Traverso, A.; Massardo, A.F. [TPG-DIMSET, University of Genoa, Via Montallegro 1, 16145 Genova (Italy); Cerutti, F.; Costamagna, P. [TPG-DICHEP, University of Genoa, Via Opera Pia 15, 16145 Genova (Italy); Bozzolo, M. [Rolls-Royce Fuel Cell Systems Ltd, PO Box 31, Derby DE24 8BJ (United Kingdom)

    2005-02-01

    This work describes different models, developed by the Thermochemical Power Group at the University of Genoa (Italy), for the simulation of solid oxide fuel cell and gas turbine hybrid systems. The paper focuses on both ''cores'' of the system: the fuel cell stack on the one hand and the turbomachinery and the auxiliaries on the other hand. Therefore, in the first part of the paper the models developed for the analysis of the Rolls-Royce Integrated Planar SOFC cells are presented; the results are compared to experimental data, and carefully analysed and discussed. In the second part of the paper, design and off design models of IP-SOFC pressurised hybrid systems in the range 250 kW-20 MW are presented; the hybrid performance results are presented and discussed, also taking ambient condition effects and a possible control strategy into account. Finally, using an in-house general purpose transient system analysis code (TRANSEO code), where chemical composition, heat transfer, and fluid dynamic influences vs. time are considered in detail, a preliminary time dependent investigation of a pressurised hybrid system behaviour is presented. (Abstract Copyright [2005], Wiley Periodicals, Inc.)

  6. Mobile IP: Security & application

    NARCIS (Netherlands)

    Tuquerres, Gloria; Salvador, Marcos Rogério; Sprenkels, Ron

    1999-01-01

    As required in the TGS Mobile IP Advanced Module, this paper presents a survey of common security threats which mobile IP networks are exposed to as well as some proposed solutions to deal with such threats.

  7. A model for the parabolic slices Per1(e2πip/q) in moduli space of quadratic rational maps

    DEFF Research Database (Denmark)

    Uhre, Eva

    2010-01-01

    The notion of relatedness loci in the parabolic slices Per1(e2πip/q) in moduli space of quadratic rational maps is introduced. They are counterparts of the disconnectedness or escape locus in the slice of quadratic polynomials. A model for these loci is presented, and a strategy of proof of the f...

  8. Theoretical mean-variance relationship of IP network traffic based on ON/OFF model

    Institute of Scientific and Technical Information of China (English)

    JIN Yi; ZHOU Gang; JIANG DongChen; YUAN Shuai; WANG LiLi; CAO JianTing

    2009-01-01

    Mean-variance relationship (MVR), nowadays agreed in power law form, is an important function. It Is currently used by traffic matrix estimation as a basic statistical assumption. Because all the existing papers obtain MVR only through empirical ways, they cannot provide theoretical support to power law MVR or the definition of its power exponent. Furthermore, because of the lack of theoretical model, all traffic matrix estimation methods based on MVR have not been theoretically supported yet. By observ-ing both our laboratory and campus network for more than one year, we find that such an empirical MVR is not sufficient to describe actual network traffic. In this paper, we derive a theoretical MVR from ON/OFF model. Then we prove that current empirical power law MVR is generally reasonable by the fact that it is an approximate form of theoretical MVR under specific precondition, which can theoretically support those traffic matrix estimation algorithms of using MVR. Through verifying our MVR by actual observation and public DECPKT traces, we verify that our theoretical MVR Is valid and more capable of describing actual network traffic than power law MVR.

  9. Ensuring Software IP Cleanliness

    Directory of Open Access Journals (Sweden)

    Mahshad Koohgoli

    2007-12-01

    Full Text Available At many points in the life of a software enterprise, determination of intellectual property (IP cleanliness becomes critical. The value of an enterprise that develops and sells software may depend on how clean the software is from the IP perspective. This article examines various methods of ensuring software IP cleanliness and discusses some of the benefits and shortcomings of current solutions.

  10. iPS Cells for Modelling and Treatment of Retinal Diseases

    Directory of Open Access Journals (Sweden)

    Fred K. Chen

    2014-12-01

    Full Text Available For many decades, we have relied on immortalised retinal cell lines, histology of enucleated human eyes, animal models, clinical observation, genetic studies and human clinical trials to learn more about the pathogenesis of retinal diseases and explore treatment options. The recent availability of patient-specific induced pluripotent stem cells (iPSC for deriving retinal lineages has added a powerful alternative tool for discovering new disease-causing mutations, studying genotype-phenotype relationships, performing therapeutics-toxicity screening and developing personalised cell therapy. This review article provides a clinical perspective on the current and potential benefits of iPSC for managing the most common blinding diseases of the eye: inherited retinal diseases and age-related macular degeneration.

  11. IMPLEMENTASI MODEL PEMBELAJARAN BERBASIS MASALAH LOKAL DALAM MENGEMBANGKAN KOMPETENSI EKOLOGIS PADA PEMBELAJARAN IPS

    Directory of Open Access Journals (Sweden)

    Muhaimin Muhaimin

    2015-11-01

    Full Text Available Human and the environment is an integrated system to form ecosystems in which humans are physically and socially dependent. One concern is the content of social studies related to the ecological interactions between humans and the environment. In the factual context, ecological competence developed in social studies only at the level of knowlegde ecological, moral, and behavior, which has not been valued and embodied in the form of concrete to protect and sustain the environment. The results showed no differences in ecological competence for aspects of knowledge, attitudes, and skills between the pre-test and post-test. The study recommends that the implementation of learning social science education in a broader scope based on local environmental issues are found in everyday life. Schools cultivate ecological competence through the formation of habits, role modeling, and school behavior and culture form a joint motion which is in line with the curriculum green and green living. Permalink/DOI: http://dx.doi.org/10.15408/sd.v2i1.1409

  12. Landscape-scale Ips typographus attack dynamics: from monitoring plots to GIS-based disturbance models

    Directory of Open Access Journals (Sweden)

    Schlyter F

    2011-12-01

    Full Text Available In natural spruce stands, a change of generation is usually initiated by wind or bark beetle disturbances. We combined semi-temporary monitoring plots, remote sensing, and GIS in order to understand and model these processes. Sub plots, called “active”, were located in areas with a high probability of bark beetle or wind disturbances. The optimal location of these plots is usually at an active forest edge, i.e., the zone of maximal change in bark beetle abundance over time, corresponding to the border between wind-damaged or bark beetle-attacked parts and undisturbed parts of a forest stand. The key variable investigated was tree mortality caused by bark beetles. Other variables were similar to those recorded in traditional forest monitoring. Tree defense indicators (resin flow, phenolic compounds and reaction of a tree to bark beetle inoculation were measured. Terrestrial data were then combined with remote sensing data. Time series of satellite images were analyzed in order to define the pattern of wind and bark beetle damages. Weather monitoring data were used for predicting bark beetle and water stress development. All of the information was integrated in a GIS-based system and future bark beetle infestations were predicted. In this paper, we review previous studies and conclude that: (1 the hypotheses of habitat selection (non-host volatiles and semiochemical diversity and location of moderately-stressed host trees are confirmed, although further work about olfactory orientation and host resistance is needed;(2 reactions of trees to bark beetle attack can be predicted by monitoring several parameters, e.g., air temperature and tree physiology; (3 data from ground monitoring can be integrated with GIS and remote sensing systems for bark beetle prognosis and management at the habitat and landscape levels.

  13. Model of intrusion prevention system based on dynamic IP blacklist%基于动态IP黑名单的入侵防御系统模型

    Institute of Scientific and Technical Information of China (English)

    卢先锋; 杨频; 梁刚

    2011-01-01

    通过分析网络入侵防御系统(network intrusion prevention system,NIPS)在处理超大网络攻击流量时存在的不足和性能瓶颈,提出并建立一种基于动态IP黑名单技术的NIPS模型,使NIPS维护一个存储攻击主机IP地址和其威胁度信息的哈希表,通过威胁度评估算法周期计算攻击源IP的威胁度并更新到哈希表内,预过滤模块将根据IP黑名单和过滤策略来对整个网络数据包进行预先过滤.实验结果表明,该模型在处理超大网络攻击流量时比传统NIPS更加快速高效,并且能更好地保护NIPS身后的网络.%NIPS (network intrusion prevention systems) often has a high loss rate and low-performance when handling the huge attack traffic.A model of NIPS based on dynamic IP blacklist is proposed to solve this problems.NIPS maintain a hash table (IP blacklist)while keep the attacker's IP address and their threat values.Through the attacker's IP's threat evaluation algorithm, NIPS will periodically compute the attacker's threat values and save them into the hash table.While huge network flow reaches, NIPS can rapidly filter the flow beforehand by means ofusing the IP blacklist hash table.The experiments showed that using this model can improve the performance of NIPS and make the protected network safer.

  14. iPS cell modeling of Best disease: insights into the pathophysiology of an inherited macular degeneration.

    Science.gov (United States)

    Singh, Ruchira; Shen, Wei; Kuai, David; Martin, Jessica M; Guo, Xiangrong; Smith, Molly A; Perez, Enio T; Phillips, M Joseph; Simonett, Joseph M; Wallace, Kyle A; Verhoeven, Amelia D; Capowski, Elizabeth E; Zhang, Xiaoqing; Yin, Yingnan; Halbach, Patrick J; Fishman, Gerald A; Wright, Lynda S; Pattnaik, Bikash R; Gamm, David M

    2013-02-01

    Best disease (BD) is an inherited degenerative disease of the human macula that results in progressive and irreversible central vision loss. It is caused by mutations in the retinal pigment epithelium (RPE) gene BESTROPHIN1 (BEST1), which, through mechanism(s) that remain unclear, lead to the accumulation of subretinal fluid and autofluorescent waste products from shed photoreceptor outer segments (POSs). We employed human iPS cell (hiPSC) technology to generate RPE from BD patients and unaffected siblings in order to examine the cellular and molecular processes underlying this disease. Consistent with the clinical phenotype of BD, RPE from mutant hiPSCs displayed disrupted fluid flux and increased accrual of autofluorescent material after long-term POS feeding when compared with hiPSC-RPE from unaffected siblings. On a molecular level, RHODOPSIN degradation after POS feeding was delayed in BD hiPSC-RPE relative to unaffected sibling hiPSC-RPE, directly implicating impaired POS handling in the pathophysiology of the disease. In addition, stimulated calcium responses differed between BD and normal sibling hiPSC-RPE, as did oxidative stress levels after chronic POS feeding. Subcellular localization, fractionation and co-immunoprecipitation experiments in hiPSC-RPE and human prenatal RPE further linked BEST1 to the regulation and release of endoplasmic reticulum calcium stores. Since calcium signaling and oxidative stress are critical regulators of fluid flow and protein degradation, these findings likely contribute to the clinical picture of BD. In a larger context, this report demonstrates the potential to use patient-specific hiPSCs to model and study maculopathies, an important class of blinding disorders in humans.

  15. Integrative Analysis of PRKAG2 Cardiomyopathy iPS and Microtissue Models Identifies AMPK as a Regulator of Metabolism, Survival, and Fibrosis

    Directory of Open Access Journals (Sweden)

    J. Travis Hinson

    2016-12-01

    Full Text Available AMP-activated protein kinase (AMPK is a metabolic enzyme that can be activated by nutrient stress or genetic mutations. Missense mutations in the regulatory subunit, PRKAG2, activate AMPK and cause left ventricular hypertrophy, glycogen accumulation, and ventricular pre-excitation. Using human iPS cell models combined with three-dimensional cardiac microtissues, we show that activating PRKAG2 mutations increase microtissue twitch force by enhancing myocyte survival. Integrating RNA sequencing with metabolomics, PRKAG2 mutations that activate AMPK remodeled global metabolism by regulating RNA transcripts to favor glycogen storage and oxidative metabolism instead of glycolysis. As in patients with PRKAG2 cardiomyopathy, iPS cell and mouse models are protected from cardiac fibrosis, and we define a crosstalk between AMPK and post-transcriptional regulation of TGFβ isoform signaling that has implications in fibrotic forms of cardiomyopathy. Our results establish critical connections among metabolic sensing, myocyte survival, and TGFβ signaling.

  16. Affordable Window Insulation with R-10/inch Rating

    Energy Technology Data Exchange (ETDEWEB)

    Jenifer Marchesi Redouane Begag; Je Kyun Lee; Danny Ou; Jong Ho Sonn; George Gould; Wendell Rhine

    2004-10-15

    During the performance of contract DE-FC26-00-NT40998, entitled ''Affordable Window Insulation with R-10/inch Value'', research was conducted at Aspen Aerogels, Inc. to develop new transparent aerogel materials suitable for window insulation applications. The project requirements were to develop a formulation or multiple formulations that have high transparency (85-90%) in the visible region, are hydrophobic (will not opacify with exposure to water vapor or liquid), and have at least 2% resiliency (interpreted as recoverable 2% strain and better than 5% strain to failure in compression). Results from an unrelated project showed that silica aerogels covalently bonded to organic polymers exhibit excellent mechanical properties. At the outset of this project, we believed that such a route is the best to improve mechanical properties. We have applied Design of Experiment (DOE) techniques to optimize formulations including both silica aerogels and organically modified silica aerogels (''Ormosils''). We used these DOE results to optimize formulations around the local/global optimization points. This report documents that we succeeded in developing a number of formulations that meet all of the stated criteria. We successfully developed formulations utilizing a two-step approach where the first step involves acid catalyzed hydrolysis and the second step involves base catalyzed condensation to make the gels. The gels were dried using supercritical CO{sub 2} and we were able to make 1 foot x 1 foot x 0.5 inch panels that met the criteria established.

  17. IP Routing Protocols

    Science.gov (United States)

    Nolasco Pinto, Armando

    2002-03-01

    Uyless Black is a widely known expert in computer networks and data communications. He is author of more than ten books in the communication technologies field, which puts him in a good position to address this topic. In IP Routing Protocols he starts by providing the background and concepts required for understanding TCP/IP technology. This is done clearly and assumes little prior knowledge of the area. As might be expected, he emphasizes the IP route discovery problem. Later he details several routing protocols.

  18. miR-10a在肿瘤中的研究进展%Research progress of miR-10a in tumors

    Institute of Scientific and Technical Information of China (English)

    支勇金

    2014-01-01

    miR-10a,as one member of miRNA family,belongs to the fannily of miR-10.It locates between HOXB4 and HOXB5 genes on the short arm of chromosome 17.miR-10a is abnormal expressed in a variety of human tumors and is closely related to the occurrence,development and prognosis of tumor.The relationship of miR-10a with a variety of tumors is reviewed in this paper.%miR-10a作为微小RNA(miRNA)家族的成员之一,属于miR-10家族,定位于17号染色体短臂的HOXB4与HOXB5基因之间.miR-10a在多种人体肿瘤中异常表达,与肿瘤的发生、发展及预后密切相关.文章就miR-10a与多种肿瘤的关系进行综述.

  19. miR-10b promotes invasion by targeting KLF4 in osteosarcoma cells.

    Science.gov (United States)

    Wang, Jing; Wang, Bing; Chen, Ling-Qiang; Yang, Jin; Gong, Zhi-Qiang; Zhao, Xue-Ling; Zhang, Chun-Qiang; Du, Kai-Li

    2016-12-01

    Osteosarcoma is a common malignancy with high rate of metastasis. miR-10b has been reported to be expressed in many types of tumors abnormally and be associated with cancer carcinogenesis and progression. But the function of miR-10b in osteosarcoma is still unknown. So this study was aimed to investigate the role of miR-10b in osteosarcoma development. miR-10b expression in osteosarcoma tissues and osteosarcoma cells were detected using real time PCR. The effects of miR-10b on osteosarcoma cells proliferation, apoptosis, migration and invasion were detected using CCK-8 assay, flow cytometry, wound-healing assay and transwell assay, respectively. The relationship between miR-10b and KLF4 was evaluated using dual-luciferase assay, correlation analysis. miR-10b was highly expressed in osteosarcoma tissues and osteosarcoma cells. Furthermore, inhibition of miR-10b in osteosarcoma cells depressed the cells proliferation, migration and invasion but promoted cells apoptosis. In addition, KLF4 was down-regulated by miR-10b and miR-10b expression was negatively related to KLF4 expression in osteosarcoma tissue, miR-10b participated in the process of osteosarcoma cells invasion by regulating KLF4 expression. miR-10b is overexpressed in osteosarcoma and KLF4 is the direct target gene of miR-10b. Furthermore, miR-10b promotes osteosarcoma cells progression by downregulating KLF4 expression. These results suggest that miR-10b functions as an oncomiR and play an important role in osteosarcoma cellular processes at least partially through regulating KLF4; miR-10b may be a therapeutic target for osteosarcoma treatment. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  20. Voice over IP

    OpenAIRE

    Mantula, Juha

    2006-01-01

    Tämä opinnäytetyö käsittelee Voice over Internet Protocol -tekniikkaa ja sen tuomia mahdollisuuksia yrityselämässä. Teoriaosa käsittelee VoIP:n kannalta tärkeitä pro-tokollia ja standardeja, VoIP:n ominaisuuksia sekä esittelee erilaisia puheohjelmia, jotka käyttävät VoIP-tekniikkaa hyväkseen. Empiirinen osuus tutkii Viestintä Ky Pitkärannan Skype-ohjelman käyttöä. Työn tarkoituksena on selvittää VoIP:n hyviä ja huonoja puolia ja sitä kuinka tek-niikkaa voidaan käyttää hyväksi päivittäisessä ...

  1. Wireless IP Telephony

    CERN Document Server

    Gerami, Mohsen

    2010-01-01

    The convergence of traditional telecommunications and the Internet is creating new network-based service delivery opportunities for telecommunications companies carriers, service providers, and network equipment providers. Voice over Wireless IP is one of the most exciting new developments emerging within the telephony market. It is set to revolutionize the delivery of mobile voice Services and provide exciting new opportunities for operators and service providers alike. This survey discusses principal of Wireless IP Telephony.

  2. PENGELOLAAN PEMBELAJARAN PRAKTEK AKUNTANSI MENGGUNAKAN MODEL KONTEKTUAL UNTUK MENINGKATKAN KOMPETENSI SISWA PADA MATA PELAJARAN AKUNTANSI DI KELAS XI IPS 1 SMA NEGERI 7 BOGOR

    Directory of Open Access Journals (Sweden)

    Tuti Herawati

    2010-08-01

    Full Text Available This research aim to develop management of study of practice of accountancy applies contextual model which can be exploited in process of study to increase student interest at accountancy subject. This research done in class XI IPS1 SMAN 7 Bogor applies Classroom Action Research contextual model consisted of two cycles to refer to Model Kurt Lewin its (the activity covering: (1 planning, (2 action of execution, (3 observation, and ( 4 reflection. Based on finding result of research indicates that: (1 In executing management of study of practice of teacher accountancy have been able to apply contextual model to increase attainment of student learning interest; this thing is visible result of first cycle interest test result has not shown Criteria Complete Minimal (KKM, while second cycle interest test result hardly gladdens because result of acquirement above indicator Criteria Complete Minimal (KKM what specified. (2 Approach study plan of contextual correctly can be exploited in class XI IPS SMAN 7 that is management of study of practice of accountancy applies contextual model and student spread sheet as study guidance referring to study indicator. (3 Study activity is done not only in class but for study activity of accountancy computer can apply computer laboratory. (4 Percentage of enthusiastic and involvement of student learning at showing second cycle is improvement, this thing is a real good result mean attention of student to study and can entangle itself in study.

  3. Beyond iPS!

    Directory of Open Access Journals (Sweden)

    Editorial

    2012-01-01

    Full Text Available It’s undoubtedly a jubilant moment for scientists and clinicians working in the stem cell arena as Prof. Gurdon and Prof. Shinya Yamanaka have been chosen for the Nobel Prize in Physiology & Medicine this year. The mystery of cell biology is something unfathomable and probably the work of this duo as well as the other scientists, who have put their hands on in- vitro de-differentiation have opened our eyes to a new window or a new paradigm in cell biology. The iPS invention has brought a lot of hope in terms of potential direct benefits to treat several diseases, which have no definite options at the moment. But, we envisage that several spin-offs could come out of this invention and one very significant spin-off finding recently witnessed is the finding by Prof. Masaharu Seno and his team of researchers at the Okayama University, Japan (Chen L, et al. 2012, PLoS ONE 7(4:e33544.doi:10.1371/journal.pone.0033544. According to Prof. Seno, mouse iPS cells (miPS when cultured in the conditioned medium derived from cancer cell lines, differentiate into cancer stem cells (CSCs. While differentiating into CSCs, they do retain the potential to develop endothelial progenitor cells. Several questions arise here: 1.Are these miPS derived CSCs really pluripotent, even if the terminal differentiation destined to specific phenotypes? 2.Shouldn’t the Cancer Stem Cells be termed as cancer progenitor cells, as till date they are considered to be producing only cancer cells but not pluripotent to yield other types of normal tissues? The spin-offs could be infinite as the process of differentiation and de-differentiation happening due to trillions of signals and pathways, most still remaining not-so-well understood. A special mention should be made to Prof. Shinya Yamanaka as he has several sterling qualities to be a role-model for budding scientists. Apart from his passion for science, which made him shift his career from orthopedics to a cell biologist, his

  4. Inhibitory effects of selected dietary flavonoids on the formation of total heterocyclic amines and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in roast beef patties and in chemical models.

    Science.gov (United States)

    Zhu, Qin; Zhang, Shuang; Wang, Mingfu; Chen, Jie; Zheng, Zong-Ping

    2016-02-01

    In this study, the inhibitory effects of eight kinds of dietary flavonoids on the formation of heterocyclic amines (HAs) were investigated in roast beef patties. The results showed that most of them exhibited significant inhibition on both total HAs and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), one of the most abundant HAs. Among the studied flavonoids, phlorizin, epigallocatechin gallate (EGCG), and quercetin were found to be the most effective in both the reductions of total HAs (55-70%) and PhIP (60-80%). The reaction activity between the flavonoid and phenylacetaldehyde, a key intermediate in PhIP formation, showed a good correlation with the inhibition of PhIP formation in an aqueous model system (R(2) = 0.8904) and a di(ethylene) glycol reaction system (R(2) = 0.6514). However, no significant correlation was found between the flavonoid antioxidant capacity and PhIP formation (R(2) = 0.2359). The postulated adducts of flavonoids-phenylacetaldehyde were further confirmed by LC-MS analysis in the chemical models. Since phenylacetaldehyde is the chief intermediate in PhIP formation, these results suggest that the inhibitory effects of flavonoids on PhIP formation are mainly dependent on their abilities to trap phenylacetaldehyde as opposed to their antioxidant capacities.

  5. Loss of miR-10a activates lpo and collaborates with activated Wnt signaling in inducing intestinal neoplasia in female mice.

    Directory of Open Access Journals (Sweden)

    Gustavo Stadthagen

    2013-10-01

    Full Text Available miRNAs are small regulatory RNAs that, due to their considerable potential to target a wide range of mRNAs, are implicated in essentially all biological process, including cancer. miR-10a is particularly interesting considering its conserved location in the Hox cluster of developmental regulators. A role for this microRNA has been described in developmental regulation as well as for various cancers. However, previous miR-10a studies are exclusively based on transient knockdowns of this miRNA and to extensively study miR-10a loss we have generated a miR-10a knock out mouse. Here we show that, in the Apc(min mouse model of intestinal neoplasia, female miR-10a deficient mice develop significantly more adenomas than miR-10(+/+ and male controls. We further found that Lpo is extensively upregulated in the intestinal epithelium of mice deprived of miR-10a. Using in vitro assays, we demonstrate that the primary miR-10a target KLF4 can upregulate transcription of Lpo, whereas siRNA knockdown of KLF4 reduces LPO levels in HCT-116 cells. Furthermore, Klf4 is upregulated in the intestines of miR-10a knockout mice. Lpo has previously been shown to have the capacity to oxidize estrogens into potent depurinating mutagens, creating an instable genomic environment that can cause initiation of cancer. Therefore, we postulate that Lpo upregulation in the intestinal epithelium of miR-10a deficient mice together with the predominant abundance of estrogens in female animals mainly accounts for the sex-related cancer phenotype we observed. This suggests that miR-10a could be used as a potent diagnostic marker for discovering groups of women that are at high risk of developing colorectal carcinoma, which today is one of the leading causes of cancer-related deaths.

  6. Gene expression profiling upon (212) Pb-TCMC-trastuzumab treatment in the LS-174T i.p. xenograft model.

    Science.gov (United States)

    Yong, Kwon J; Milenic, Diane E; Baidoo, Kwamena E; Kim, Young-Seung; Brechbiel, Martin W

    2013-10-01

    Recent studies have demonstrated that therapy with (212) Pb-TCMC-trastuzumab resulted in (1) induction of apoptosis, (2) G2/M arrest, and (3) blockage of double-strand DNA damage repair in LS-174T i.p. (intraperitoneal) xenografts. To further understand the molecular basis of the cell killing efficacy of (212) Pb-TCMC-trastuzumab, gene expression profiling was performed with LS-174T xenografts 24 h after exposure to (212) Pb-TCMC-trastuzumab. DNA damage response genes (84) were screened using a quantitative real-time polymerase chain reaction array (qRT-PCR array). Differentially regulated genes were identified following exposure to (212) Pb-TCMC-trastuzumab. These included genes involved in apoptosis (ABL, GADD45α, GADD45γ, PCBP4, and p73), cell cycle (ATM, DDIT3, GADD45α, GTSE1, MKK6, PCBP4, and SESN1), and damaged DNA binding (DDB) and repair (ATM and BTG2). The stressful growth arrest conditions provoked by (212) Pb-TCMC-trastuzumab were found to induce genes involved in apoptosis and cell cycle arrest in the G2/M phase. The expression of genes involved in DDB and single-strand DNA breaks was also enhanced by (212) Pb-TCMC-trastuzumab while no modulation of genes involved in double-strand break repair was apparent. Furthermore, the p73/GADD45 signaling pathway mediated by p38 kinase signaling may be involved in the cellular response, as evidenced by the enhanced expression of genes and proteins of this pathway. These results further support the previously described cell killing mechanism by (212) Pb-TCMC-trastuzumab in the same LS-174T i.p. xenograft. Insight into these mechanisms could lead to improved strategies for rational application of radioimmunotherapy using α-particle emitters.

  7. IP over DWDM

    Science.gov (United States)

    Allen, Mark E.

    2003-04-01

    The industry has been full of many lively debates about exactly how the functions of layer 1 and 2 (physical layer and data-link layer) will be orchestrated to support the growing amount of IP traffic. In effect, this book must simultaneously address layers 1 through 3. Two main strengths of the book are in describing current state-of-the-art research in very-high-speed IP networks and in detailing the standards activities related to net-work management, software, and signaling.

  8. PERBEDAAN HASIL BELAJAR EKONOMI SISWA YANG MENGGUNAKAN MODEL PEMBELAJARANKOOPERATIFTIPETHINK PAIR SHARE (TPS DENGAN TIPE STUDENT TEAMS ACHIEVEMENT DIVISION(STADSISWA KELAS XI IPS SMAN 11 PADANG

    Directory of Open Access Journals (Sweden)

    Dina Sofianti

    2013-04-01

    Full Text Available The research aims to analyze the difference of learning points by using the cooperative learning models between Think Pair Share (TPS and Student Teams Achievement Division (STAD types of the XI-grade students of IPS SMAN 11 Padang. It is an experimental research. The population of this research is all XI-grade students of IPS SMAN 11 Padang, who were registered in the year 2013-2014. Two samples were selected by using the technique of Purposive Sampling method. The data of this research is primary data, and the data analyses are descriptive and inductive, conducted through t-test by first making tests of normality and homogeneity of both sample variants. Based on the data analyses, it is obtained that the learning points for the experimental class 1 is 76.80 and the experimental class 2 is 65.64 on the average, meaning that Sig 0.018 is smaller than α = 0.05. From the hypothesis tests, tcalc.is 2.444 and ttable is 1.67, so that H0 is rejected, meaning that there is the difference between TPS and STAD. From the results, it is expected that this could provide a solution for the problem of lowering learning points especially in economics subject. Finally, for improving the learning points of economical students, it is suggested for teachers to consider the learning models from Think Pair Share TPS and STAD.

  9. MiR-10 represses HoxB1a and HoxB3a in zebrafish.

    Directory of Open Access Journals (Sweden)

    Joost M Woltering

    Full Text Available BACKGROUND: The Hox genes are involved in patterning the anterior-posterior axis. In addition to the protein coding Hox genes, the miR-10, miR-196 and miR-615 families of microRNA genes are conserved within the vertebrate Hox clusters. The members of the miR-10 family are located at positions associated with Hox-4 paralogues. No function is yet known for this microRNA family but the genomic positions of its members suggest a role in anterior-posterior patterning. METHODOLOGY/PRINCIPAL FINDINGS: Using sensor constructs, overexpression and morpholino knockdown, we show in Zebrafish that miR-10 targets HoxB1a and HoxB3a and synergizes with HoxB4 in the repression of these target genes. Overexpression of miR-10 also induces specific phenotypes related to the loss of function of these targets. HoxB1a and HoxB3a have a dominant hindbrain expression domain anterior to that of miR-10 but overlap in a weaker expression domain in the spinal cord. In this latter domain, miR-10 knockdown results in upregulation of the target genes. In the case of a HoxB3a splice variant that includes miR-10c within its primary transcript, we show that the microRNA acts in an autoregulatory fashion. CONCLUSIONS/SIGNIFICANCE: We find that miR-10 acts to repress HoxB1a and HoxB3a within the spinal cord and show that this repression works cooperatively with HoxB4. As with the previously described interactions between miR-196 and HoxA7 and Hox-8 paralogues, the target genes are located in close proximity to the microRNA. We present a model in which we postulate a link between the clustering of Hox genes and post-transcriptional gene regulation. We speculate that the high density of transcription units and enhancers within the Hox clusters places constraints on the precision of the transcriptional control that can be achieved within these clusters and requires the involvement of post-transcriptional gene silencing to define functional domains of genes appropriately.

  10. Safeguarding nonhuman primate iPS cells with suicide genes.

    Science.gov (United States)

    Zhong, Bonan; Watts, Korashon L; Gori, Jennifer L; Wohlfahrt, Martin E; Enssle, Joerg; Adair, Jennifer E; Kiem, Hans-Peter

    2011-09-01

    The development of technology to generate induced pluripotent stem (iPS) cells constitutes one of the most exciting scientific breakthroughs because of the enormous potential for regenerative medicine. However, the safety of iPS cell-related products is a major concern for clinical translation. Insertional mutagenesis, possible oncogenic transformation of iPS cells or their derivatives, or the contamination of differentiated iPS cells with undifferentiated cells, resulting in the formation of teratomas, have remained considerable obstacles. Here, we demonstrate the utility of suicide genes to safeguard iPS cells and their derivatives. We found suicide genes can control the cell fate of iPS cells in vitro and in vivo without interfering with their pluripotency and self-renewal capacity. This study will be useful to evaluate the safety of iPS cell technology in a clinically highly relevant, large animal model and further benefit the clinical use of human iPS cells.

  11. Mobile IP and protocol authentication extension

    CERN Document Server

    Nguyen, Phuc V

    2011-01-01

    Mobile IP is an open standard, defined by the Internet Engineering Task Force (IETF) RFC 3220. By using Mobile IP, you can keep the same IP address, stay connected, and maintain ongoing applications while roaming between IP networks. Mobile IP is scalable for the Internet because it is based on IP - any media that can support IP can support Mobile IP.

  12. Digital signage sobre IP

    OpenAIRE

    Casademont Filella, Albert

    2012-01-01

    Projecte que versa sobre el disseny i la implementació d'un sistema de Digital Signage (Cartelleria Digital) sobre IP, creant un gestor que permeti controlar de forma remota tot un conjunt de petits dispositius connectats a pantalles que emeten continguts multimèdia com vídeos, imatges, feeds rss...

  13. On Principles of IP Phone Technology and Its Future%IP Phone的技术原理及展望

    Institute of Scientific and Technical Information of China (English)

    张祖良; 杨佩林; 陈刚

    2001-01-01

    This paper introduces principles of IP Phone technology. The basic communication model of IP Phone and the framework of connecting PSTN and Internet are presented. It also describes the hardware and software of an IP Phone gateway as well as protocols involving IP Phone core technology. Finally, it makes an analysis on the prospects.%本文介绍了IP Phone技术的发展及技术原理。IP Phone的基本通信模式,与现存的电话网络及国际互联网的连接框架结构。介绍了IP网关的构成所涉及的软硬件技术以及IP Phone的核心技术及涉及的协议规范。最后对IP Phone技术的发展前景进行了科学的分析。

  14. Combining ChIP-chip and expression profiling to model the MoCRZ1 mediated circuit for Ca/calcineurin signaling in the rice blast fungus.

    Directory of Open Access Journals (Sweden)

    Soonok Kim

    2010-05-01

    Full Text Available Significant progress has been made in defining the central signaling networks in many organisms, but collectively we know little about the downstream targets of these networks and the genes they regulate. To reconstruct the regulatory circuit of calcineurin signal transduction via MoCRZ1, a Magnaporthe oryzae C2H2 transcription factor activated by calcineurin dephosphorylation, we used a combined approach of chromatin immunoprecipitation - chip (ChIP-chip, coupled with microarray expression studies. One hundred forty genes were identified as being both a direct target of MoCRZ1 and having expression concurrently differentially regulated in a calcium/calcineurin/MoCRZ1 dependent manner. Highly represented were genes involved in calcium signaling, small molecule transport, ion homeostasis, cell wall synthesis/maintenance, and fungal virulence. Of particular note, genes involved in vesicle mediated secretion necessary for establishing host associations, were also found. MoCRZ1 itself was a target, suggesting a previously unreported autoregulation control point. The data also implicated a previously unreported feedback regulation mechanism of calcineurin activity. We propose that calcium/calcineurin regulated signal transduction circuits controlling development and pathogenicity manifest through multiple layers of regulation. We present results from the ChIP-chip and expression analysis along with a refined model of calcium/calcineurin signaling in this important plant pathogen.

  15. PENGARUH MODEL PEMBELAJARAN AKTIF DENGAN METODE QUESTION STUDENTS HAVE TERHADAP HASIL BELAJAR EKONOMI SISWA KELAS XI IPS DI SMA NEGERI 7 DAN SMA NEGERI 8 PADANG

    Directory of Open Access Journals (Sweden)

    Jimi Ronald

    2015-07-01

    Full Text Available This research was aimed at finding the effect of using the ‘Question students Have’ method to the result of the learning between using the conventional method toward the students’ learning result in the subject of Economy. The research was a quasi-experiment whose population was grade XI IPS students of SMA negeri 7 Padang dan SMA Negeri 8 Padang. The sampling technique used was the purposive sampling. The experimental group was the grade XI IPS students of SMA Negeri 8 and the control group was Grade students of SMA Negeri 7 Padang. The data of the research consisted of two kinds : primary and secondary data. The data were analyzed by using a descriptive method and inductive analysis in the form of the ANOVA. The findings of the research were The students taught with the Question Students Have scored significantly higher than those taught with the conventional method with the significance index (Sig = 0.000 smaller than the value of 0.05 (sig. a. Based on the findings, it is suggested that the teachers use this method or model in the teaching of Economy and always motivate the students.

  16. 26 CFR 1.414(r)-10 - Separate application of section 129(d)(8). [Reserved

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 5 2010-04-01 2010-04-01 false Separate application of section 129(d)(8). 1.414(r)-10 Section 1.414(r)-10 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES Pension, Profit-Sharing, Stock Bonus Plans, Etc. §...

  17. IP and ATM Integration

    OpenAIRE

    Mannie, E.; Corridoni, J.; Cremonese, P.; Giordano, Silvia

    1999-01-01

    The performance limits of the current Internet make the integration of IP with ATM a hotly debated issue in the networking arena, leading to various competing approaches and products. Legitimate technical and market issues are, however, often intertwined with biased views and hype, with vendors competing in the standards arena as well as on the markets. Together with the speed of technical evolution, this causes confusion for purchasers of networking equipment who, usually preferring a single...

  18. Comparative calibration of IP scanning equipment

    Science.gov (United States)

    Ingenito, F.; Andreoli, P.; Batani, D.; Boutoux, G.; Cipriani, M.; Consoli, F.; Cristofari, G.; Curcio, A.; De Angelis, R.; Di Giorgio, G.; Ducret, J.; Forestier-Colleoni, P.; Hulin, S.; Jakubowska, K.; Rabhi, N.

    2016-05-01

    Imaging Plates (IP) are diagnostic devices which contain a photostimulable phosphor layer that stores the incident radiation dose as a latent image. The image is read with a scanner which stimulates the decay of electrons, previously excited by the incident radiation, by exposition to a laser beam. This results in emitted light, which is detected by photomultiplier tubes; so the latent image is reconstructed. IPs have the interesting feature that can be reused many times, after erasing stored information. Algorithms to convert signals stored in the detector to Photostimulated luminescence (PSL) counts depend on the scanner and are not available on every model. A comparative cross-calibration of the IP scanner Dürr CR35 BIO, used in ABC laboratory, was performed, using the Fujifilm FLA 7000 scanner as a reference, to find the equivalence between grey-scale values given by the Dürr scanner to PSL counts. Using an IP and a 55Fe β-source, we produced pairs of samples with the same exposition times, which were analysed by both scanners, placing particular attention to fading times of the image stored on IPs. Data analysis led us to the determine a conversion formula which can be used to compare data of experiments obtained in different laboratories and to use IP calibrations available, till now, only for Fujifilm scanners.

  19. IP Phone 综述

    Institute of Scientific and Technical Information of China (English)

    夏华; 陈常嘉

    2000-01-01

    IP Phone代表下一代电话技术,是目前的热点,它通过分组交换网络来传输话音,是开放式分组业务的一种。本文从IP Phone的出现谈起,简要介绍了IP Phone的优势,工作原理和相关标准,着重分析了IP Phone面临的挑战与机遇,并展望了IP Phone的发展前景。

  20. TCP/IP foundations

    CERN Document Server

    Blank, Andrew G

    2006-01-01

    The world of IT is always evolving, but in every area there are stable, core concepts that anyone just setting out needed to know last year, needs to know this year, and will still need to know next year. The purpose of the Foundations series is to identify these concepts and present them in a way that gives you the strongest possible starting point, no matter what your endeavor. TCP/IP Foundations provides essential knowledge about the two protocols that form the basis for the Internet, as well as many other networks. What you learn here will benefit you in the short term, as you acquire and

  1. A comparative analysis of transcription factor binding models learned from PBM, HT-SELEX and ChIP data.

    Science.gov (United States)

    Orenstein, Yaron; Shamir, Ron

    2014-04-01

    Understanding gene regulation is a key challenge in today's biology. The new technologies of protein-binding microarrays (PBMs) and high-throughput SELEX (HT-SELEX) allow measurement of the binding intensities of one transcription factor (TF) to numerous synthetic double-stranded DNA sequences in a single experiment. Recently, Jolma et al. reported the results of 547 HT-SELEX experiments covering human and mouse TFs. Because 162 of these TFs were also covered by PBM technology, for the first time, a large-scale comparison between implementations of these two in vitro technologies is possible. Here we assessed the similarities and differences between binding models, represented as position weight matrices, inferred from PBM and HT-SELEX, and also measured how well these models predict in vivo binding. Our results show that HT-SELEX- and PBM-derived models agree for most TFs. For some TFs, the HT-SELEX-derived models are longer versions of the PBM-derived models, whereas for other TFs, the HT-SELEX models match the secondary PBM-derived models. Remarkably, PBM-based 8-mer ranking is more accurate than that of HT-SELEX, but models derived from HT-SELEX predict in vivo binding better. In addition, we reveal several biases in HT-SELEX data including nucleotide frequency bias, enrichment of C-rich k-mers and oligos and underrepresentation of palindromes.

  2. MODEL PENANAMAN PENDIDIKAN KARAKTER BERBASIS NILAI-NILAI KEHIDUPAN SOSIAL PADA MATA PELAJARAN IPS DI SEKOLAH AGAMA

    Directory of Open Access Journals (Sweden)

    Siti Nurindah Sari

    2015-08-01

    Full Text Available Tujuan penelitian ini adalah mengembangan model penanaman pendidikan karakter berbasis nilai kehidupan sosial di Madrasah Tsanawiyah. Metode penelitian yang digunakan dalam penelitian ini adalah metode Research and development (R&D. Dengan lokasi penelitian  adalah Madrasah Tsanawiyah di Kabupaten Kudus. Simpulan penelitian ini menyatakan bahwa penanaman pendidikan karakter di MTs Manba’ul ulum menerapkan pendidikan karakter pada nilai religius, hal ini dikarenakan MTs Manba’ul Ulum merupakan sekolahan berbasis agama. Nilai keagamaan meliputi solat dhuha, solat duhur berjama’ah pesantren kilat dan solat idul adha. Penggunaan model penanaman pendidikan karakter berbasis nilai sosial belum pernah dilaksanakan karena kurangnya pedoman pelaksanan model bagi guru. Model penanaman pendidikan karakter berbasis nilai-nilai kehidupan sosial adalah model penanaman yang diawali dengan melakukan pengamatan pendidikan karakter yang ada dalam lokasi penelitian, kemudian mengidentifikasi permasalahan yang ada, dilanjutkan dengan melengkapi data untuk memecahkan permasalahan The purpose of this research is developing a model-based character education planting value of social life in the Islamic. The method used in this research is the method Research and development (R & D. With the study site is Islamic in Kudus. The conclusions of this study stated that the cultivation of character education in MTs Manba'ul uluum implement character education in religious values, this is because the MTs Manba'ul Ulum is faith-based schools. Religious values include Duha prayer, prayer in congregation duhur boarding lightning and Eid al-Adha prayers. The use of a model-based character education planting social values have not been implemented due to lack of guidelines for the conduct of a model for teachers.Model-based character education planting values of social life is a model of planting begins by observing the character education in the study site, then identify

  3. Bioengineering a 3D integumentary organ system from iPS cells using an in vivo transplantation model.

    Science.gov (United States)

    Takagi, Ryoji; Ishimaru, Junko; Sugawara, Ayaka; Toyoshima, Koh-Ei; Ishida, Kentaro; Ogawa, Miho; Sakakibara, Kei; Asakawa, Kyosuke; Kashiwakura, Akitoshi; Oshima, Masamitsu; Minamide, Ryohei; Sato, Akio; Yoshitake, Toshihiro; Takeda, Akira; Egusa, Hiroshi; Tsuji, Takashi

    2016-04-01

    The integumentary organ system is a complex system that plays important roles in waterproofing, cushioning, protecting deeper tissues, excreting waste, and thermoregulation. We developed a novel in vivo transplantation model designated as a clustering-dependent embryoid body transplantation method and generated a bioengineered three-dimensional (3D) integumentary organ system, including appendage organs such as hair follicles and sebaceous glands, from induced pluripotent stem cells. This bioengineered 3D integumentary organ system was fully functional following transplantation into nude mice and could be properly connected to surrounding host tissues, such as the epidermis, arrector pili muscles, and nerve fibers, without tumorigenesis. The bioengineered hair follicles in the 3D integumentary organ system also showed proper hair eruption and hair cycles, including the rearrangement of follicular stem cells and their niches. Potential applications of the 3D integumentary organ system include an in vitro assay system, an animal model alternative, and a bioengineered organ replacement therapy.

  4. iPS cell technologies and their prospect for bone regeneration and disease modeling: A mini review

    Directory of Open Access Journals (Sweden)

    Maria Csobonyeiova

    2017-07-01

    Full Text Available Bone disorders are a group of varied acute and chronic traumatic, degenerative, malignant or congenital conditions affecting the musculoskeletal system. They are prevalent in society and, with an ageing population, the incidence and impact on the population’s health is growing. Severe persisting pain and limited mobility are the major symptoms of the disorder that impair the quality of life in affected patients. Current therapies only partially treat the disorders, offering management of symptoms, or temporary replacement with inert materials. However, during the last few years, the options for the treatment of bone disorders have greatly expanded, thanks to the advent of regenerative medicine. Skeletal cell-based regeneration medicine offers promising reparative therapies for patients. Mesenchymal stem (stromal cells from different tissues have been gradually translated into clinical practice; however, there are a number of limitations. The introduction of reprogramming methods and the subsequent production of induced pluripotent stem cells provides a possibility to create human-specific models of bone disorders. Furthermore, human-induced pluripotent stem cell-based autologous transplantation is considered to be future breakthrough in the field of regenerative medicine. The main goal of the present paper is to review recent applications of induced pluripotent stem cells in bone disease modeling and to discuss possible future therapy options. The present article contributes to the dissemination of scientific and pre-clinical results between physicians, mainly orthopedist and thus supports the translation to clinical practice.

  5. Efficient and Reproducible Myogenic Differentiation from Human iPS Cells: Prospects for Modeling Miyoshi Myopathy In Vitro

    Science.gov (United States)

    Tanaka, Akihito; Woltjen, Knut; Miyake, Katsuya; Hotta, Akitsu; Ikeya, Makoto; Yamamoto, Takuya; Nishino, Tokiko; Shoji, Emi; Sehara-Fujisawa, Atsuko; Manabe, Yasuko; Fujii, Nobuharu; Hanaoka, Kazunori; Era, Takumi; Yamashita, Satoshi; Isobe, Ken-ichi; Kimura, En; Sakurai, Hidetoshi

    2013-01-01

    The establishment of human induced pluripotent stem cells (hiPSCs) has enabled the production of in vitro, patient-specific cell models of human disease. In vitro recreation of disease pathology from patient-derived hiPSCs depends on efficient differentiation protocols producing relevant adult cell types. However, myogenic differentiation of hiPSCs has faced obstacles, namely, low efficiency and/or poor reproducibility. Here, we report the rapid, efficient, and reproducible differentiation of hiPSCs into mature myocytes. We demonstrated that inducible expression of myogenic differentiation1 (MYOD1) in immature hiPSCs for at least 5 days drives cells along the myogenic lineage, with efficiencies reaching 70–90%. Myogenic differentiation driven by MYOD1 occurred even in immature, almost completely undifferentiated hiPSCs, without mesodermal transition. Myocytes induced in this manner reach maturity within 2 weeks of differentiation as assessed by marker gene expression and functional properties, including in vitro and in vivo cell fusion and twitching in response to electrical stimulation. Miyoshi Myopathy (MM) is a congenital distal myopathy caused by defective muscle membrane repair due to mutations in DYSFERLIN. Using our induced differentiation technique, we successfully recreated the pathological condition of MM in vitro, demonstrating defective membrane repair in hiPSC-derived myotubes from an MM patient and phenotypic rescue by expression of full-length DYSFERLIN (DYSF). These findings not only facilitate the pathological investigation of MM, but could potentially be applied in modeling of other human muscular diseases by using patient-derived hiPSCs. PMID:23626698

  6. Introduction à TCP/IP

    OpenAIRE

    Archimbaud, Jean-Luc

    1993-01-01

    • Ce qu'il faut savoir sur les réseaux • Ce qu'il faut savoir sur Ethernet • Généralités sur TCP/IPIP et consort • TCP et consort • Le routage • Interconnexion de réseaux Ethernet et IP • Nommage • Exemples d'applications • Enchaînement des opérations • Daemons et commandes Unix • Glossaire; Engineering school; Cours d'introduction à TCP/IP

  7. Effect of intracerebroventricular continuous infusion of valproic acid versus single i.p. and i.c.v. injections in the amygdala kindling epilepsy model.

    Science.gov (United States)

    Serralta, Alfonso; Barcia, Juan A; Ortiz, Pedro; Durán, Carmen; Hernández, M Eugenia; Alós, Manuel

    2006-07-01

    Two protocols were tested to assess anticonvulsant efficacy and drug concentrations after intracerebroventricular (i.c.v.) continuous valproic acid (VPA) infusion, as compared with acute injections in the kindling epilepsy model. Protocol 1: amygdala-kindled rats were injected via intraperitoneal (i.p.) and i.c.v. routes with varying doses of VPA and tested for seizure intensity, afterdischarge and seizure duration, ataxia and sedation. Concentrations of VPA were determined by immunofluorescence in the brain, plasma, cerebrospinal fluid (CSF) and liver in matching rats. Protocol 2: amygdala-kindled rats were implanted with osmotic minipumps containing a VPA solution in saline and connected to intraventricular catheters for 7 days. Seizure threshold, latency and duration, afterdischarge duration, ataxia and sedation were recorded daily before, during, and until 5 days after VPA infusion. In matching animals, CSF, brain, plasma and liver VPA concentration was determined. Acute i.c.v. VPA injection suppressed seizures with a remarkable ataxia and sedation. However, continuous i.c.v. infusion controlled generalised and even focal seizures without producing important side effects, high plasma levels or hepatic drug concentrations. In conclusion, continuous i.c.v. VPA infusion may protect against kindled seizures by minimising ataxia and sedation, and achieving suitable intracerebral, yet low plasma or hepatic drug concentrations, thus avoiding potential systemic toxicity.

  8. 15/16 ips Operation of the Precision Instrument Company Model P15100 tape recorder to record the standard (30 Hz) NCER seismic data multiplex system

    Science.gov (United States)

    Eaton, Jerry P.

    1976-01-01

    In recent months the need has arisen to record special seismic networks consisting of a dozen or more standard NCER seismic systems telemetered to a central collection point on a reliable, portable, low-power tape recorder. Because of its simplicity and the ease with which it can be adapted for the purpose, the PI 5100 field recorder should be considered for such use. In the tests described here, a PI 5100 was speeded up to run at 15/16 inches per second (ips) and signals from the standard multiplex system test modulator bank were recorded on one tape track by means of a simple, improvised AM record amplifier. The results of these tests are extremely encouraging: the dynamic range of the system when played back on the Bell and Howell Model 3700 B reproduce machine, with subtractive compensation, is nearly as high as for the system employing the B&H 3700 B for recording. These notes indicate the principle employed to speed up the recorder, outline the circuit required to drive the tape heads in the AM record mode, and describe the tests carried out to evaluate the system's performance.

  9. Identification of a major IP5 kinase in Cryptococcus neoformans confirms that PP-IP5/IP7, not IP6, is essential for virulence.

    Science.gov (United States)

    Li, Cecilia; Lev, Sophie; Saiardi, Adolfo; Desmarini, Desmarini; Sorrell, Tania C; Djordjevic, Julianne T

    2016-04-01

    Fungal inositol polyphosphate (IP) kinases catalyse phosphorylation of IP3 to inositol pyrophosphate, PP-IP5/IP7, which is essential for virulence of Cryptococcus neoformans. Cryptococcal Kcs1 converts IP6 to PP-IP5/IP7, but the kinase converting IP5 to IP6 is unknown. Deletion of a putative IP5 kinase-encoding gene (IPK1) alone (ipk1Δ), and in combination with KCS1 (ipk1Δkcs1Δ), profoundly reduced virulence in mice. However, deletion of KCS1 and IPK1 had a greater impact on virulence attenuation than that of IPK1 alone. ipk1Δkcs1Δ and kcs1Δ lung burdens were also lower than those of ipk1Δ. Unlike ipk1Δ, ipk1Δkcs1Δ and kcs1Δ failed to disseminate to the brain. IP profiling confirmed Ipk1 as the major IP5 kinase in C. neoformans: ipk1Δ produced no IP6 or PP-IP5/IP7 and, in contrast to ipk1Δkcs1Δ, accumulated IP5 and its pyrophosphorylated PP-IP4 derivative. Kcs1 is therefore a dual specificity (IP5 and IP6) kinase producing PP-IP4 and PP-IP5/IP7. All mutants were similarly attenuated in virulence phenotypes including laccase, urease and growth under oxidative/nitrosative stress. Alternative carbon source utilisation was also reduced significantly in all mutants except ipk1Δ, suggesting that PP-IP4 partially compensates for absent PP-IP5/IP7 in ipk1Δ grown under this condition. In conclusion, PP-IP5/IP7, not IP6, is essential for fungal virulence.

  10. Optimization of OSPF Routing in IP Networks

    Science.gov (United States)

    Bley, Andreas; Fortz, Bernard; Gourdin, Eric; Holmberg, Kaj; Klopfenstein, Olivier; Pióro, Michał; Tomaszewski, Artur; Ümit, Hakan

    The Internet is a huge world-wide packet switching network comprised of more than 13,000 distinct subnetworks, referred to as Autonomous Systems (ASs) autonomous system AS . They all rely on the Internet Protocol (IP) internet protocol IP for transport of packets across the network. And most of them use shortest path routing protocols shortest path routing!protocols , such as OSPF or IS-IS, to control the routing of IP packets routing!of IP packets within an AS. The idea of the routing is extremely simple — every packet is forwarded on IP links along the shortest route between its source and destination nodes of the AS. The AS network administrator can manage the routing of packets in the AS by supplying the so-called administrative weights of IP links, which specify the link lengths that are used by the routing protocols for their shortest path computations. The main advantage of the shortest path routing policy is its simplicity, allowing for little administrative overhead. From the network engineering perspective, however, shortest path routing can pose problems in achieving satisfactory traffic handling efficiency. As all routing paths depend on the same routing metric routing!metric , it is not possible to configure the routing paths for the communication demands between different pairs of nodes explicitly or individually; the routing can be controlled only indirectly and only as a whole by modifying the routing metric. Thus, one of the main tasks when planning such networks is to find administrative link weights that induce a globally efficient traffic routing traffic!routing configuration of an AS. It turns out that this task leads to very difficult mathematical optimization problems. In this chapter, we discuss and describe exact integer programming models and solution approaches as well as practically efficient smart heuristics for such shortest path routing problems shortest path routing!problems .

  11. Research study on IPS digital controller design

    Science.gov (United States)

    Kuo, B. C.; Folkerts, C.

    1976-01-01

    The performance is investigated of the simplified continuous-data model of the Instrument Pointing System (IPS). Although the ultimate objective is to study the digital model of the system, knowledge on the performance of the continuous-data model is important in the sense that the characteristics of the digital system should approach those of the continuous-data system as the sampling period approaches zero.

  12. Evolutionary Programming for IP/MIP Problems with Linear Constraints

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    In this paper, we propose a modified evolutionary programming with dynamic domain for solving nonlinear IP/MIP problems with linear constraints, without involving penalty function or any transformation for the problem to a linear model or others. The numerical results show that the new algorithm gives a satisfactory performance in which it works of high speed and accuracy in IP/MIP problems.

  13. Expression and significance of MMP-9, MMP-12 and IP-10 in the two emphysema models%两种肺气肿模型中MMP-9、MMP-12及IP-10的表达及意义

    Institute of Scientific and Technical Information of China (English)

    屈晓娜; 吴世满; 陈辉

    2012-01-01

    Objective To make emphysema models by active immunization and cigarette smoking using guinea pigs,then observe the expression of MMP-9,MMP-12,IP-10 and the destruction of pulmonary tissue and myocardial tissue in each model,to explore the role of the MMP-9,MMP-12,IP-10in the emphysema which induced by active immunization.Methods The guinea pigs were randomly divided into three groups,involving active immunization group,smoking group and control group.The active immunization group used the method of subcutaneous injection of elastin at multiply points and the smoking group used the traditional method,while the control group performed nothing.To observe the morphosis of pulmonary tissue and myocardial tissue and the expression of MMP-9,MMP -12,IP-10 in the pulmonary tissue and myocardial tissue of every model were observed by immunohistochemical staining,Results The expression of MMP-9,MMP-12,IP -10 in the pulmonary tissue and myocardial tissue has both significantly increased in the active immunization group and the smoking group,compared with the control group ( P <0.05).The expression of MMP-9,MMP-12 in the pulmonary tissue in the active immunization group compared with the smoking group,and there is no obvious difference ( P >0.05).The expression of matrix MMP 9,MMP-12 in the myocardial tissue in the active immunization group,compared with the smoking group ( P < 0.05).Conclusions Emphysema model of guinea pig can be established by active immunization.MMP-9,MMP-12,IP- 10 has important positive influence on emphysema which induced by active immunization.%目的 通过弹性蛋白主动免疫及熏烟制造豚鼠肺气肿模型,观察各模型中肺组织和心肌组织基质金属蛋白酶9(MMP-9)、MMP - 12及干扰素介导蛋白10(IP-10)的表达情况及组织破坏情况,探究MMP-9 、MMP- 12及IP- 10在自体免疫反应所致肺气肿形成过程中的作用.方法 将24只雄性豚鼠随机分为3组:主动免疫组、熏烟组、对照组.主动

  14. A model for peak and width of signaling windows: Ips duplicatus and Chilo partellus pheromone component proportions--does response have a wider window than production?

    Science.gov (United States)

    Schlyter, F; Svensson, M; Zhang, Q H; Knízek, M; Krokene, P; Ivarsson, P; Birgersson, G

    2001-07-01

    Pheromone communication systems have a reliable signal with a restricted window of amounts and ratios released and perceived. We propose a model based on a Gaussian response profile that allows a quantification of the response peak (location of optimum) and a measure of the peak width (response window). Interpreting the Gaussian curve, fitted by nonlinear regression (NLR), as a standard normal distribution, the peak location equals the mean (it) and the window width equals 2 x the standard deviation (2sigma). The NLR procedure can provide an objective measure for both peak location and width for a wide range of data sets. Four empirical data sets as well as 10 literature data sets were analyzed. The double-spined spruce engraver, Ips duplicatus, was field tested in four populations to find the optimum proportion for attraction to the two male aggregation pheromone components, ipsdienol (Id) and (E)-myrcenol(EM), ranging from 0 to 100% of Id. Tests in Norway and the Czech Republic confirmed the preference of western populations for a blend between 50 and 90% Id. A population in Inner Mongolia showed a preference for traps with the 10 and 50% Id baits. The NLR fitted values for response peak and width (mu; 2sigma) were: Norway 0.64, 0.73; Czech Republic 0.53, 0.73; NE China 0.77, 0.29; and Inner Mongolia 0.33, 0.50. The signal produced by Norwegian field-collected males had a narrower window width (2sigma = 0.12). Males of the maize stem borer, Chilo partellus, were tested in a flight tunnel for their response to variation in the two major female sex pheromone gland components, (Z)- l1-hexadecenal and the corresponding alcohol (OH). Variation of the alcohol in seven levels from 2 to 29% OH showed the highest male response for 17% OH. For all behavioral steps, the peak of male response was near mu = 0.14, while the window width fell from 2sigma = 0.5 to 0.2 for eight sequential behavioral steps from take-off to copulation. Female production had a similar peak location

  15. 77 FR 58410 - Commerce in Explosives; List of Explosive Materials (2012R-10T)

    Science.gov (United States)

    2012-09-20

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF JUSTICE Bureau of Alcohol, Tobacco, Firearms, and Explosives Commerce in Explosives; List of Explosive Materials (2012R-10T) AGENCY: Bureau of Alcohol, Tobacco, Firearms, and Explosives (ATF), Department of...

  16. Gevaar VoIP voor telecomsector overdreven

    NARCIS (Netherlands)

    Deventer, M.O. van; Wegberg, M. van

    2004-01-01

    Het is een hype Voice-over-IP (VoIP) voor te stellen als een ontwrichtende technologie die de telecomindustrie ingrijpend zal veranderen. Maar hoe ontwrichtend is VoIP eigenlijk? Oskar van Deventer en Marc van Wegberg analyseren drie vormen van VoIP en laten zien dat alleen ‘VoIP-chat’ potentieel

  17. Dynamic IP Reputation from DNS

    Science.gov (United States)

    2016-06-21

    management systems for Internet principals and services – E.g., IP addresses, DNS domains/servers, BGP/AS, etc. – Avoid connections to/from malicious...Operational; data sources for dynamic trust management – Dynamic IP reputation using DNS data 11/4/2009 1 Overview and Motivation • Dynamic Domain...it hosts a few number of bad domains • Ultimate goal: – An on-line dynamic trust/reputation service for IP/Domain 11/4/2009 19 Credits and

  18. Rhinovirus-16 induced release of IP-10 and IL-8 is augmented by Th2 cytokines in a pediatric bronchial epithelial cell model.

    Directory of Open Access Journals (Sweden)

    Julie A Cakebread

    Full Text Available BACKGROUND: In response to viral infection, bronchial epithelial cells increase inflammatory cytokine release to activate the immune response and curtail viral replication. In atopic asthma, enhanced expression of Th2 cytokines is observed and we postulated that Th2 cytokines may augment the effects of rhinovirus-induced inflammation. METHODS: Primary bronchial epithelial cell cultures from pediatric subjects were treated with Th2 cytokines for 24 h before infection with RV16. Release of IL-8, IP-10 and GM-CSF was measured by ELISA. Infection was quantified using RTqPCR and TCID50. Phosphatidyl inositol 3-kinase (PI3K and P38 mitogen activated protein kinase (MAPK inhibitors and dexamethasone were used to investigate differences in signaling pathways. RESULTS: The presence of Th2 cytokines did not affect RV replication or viral titre, yet there was a synergistic increase in IP-10 release from virally infected cells in the presence of Th2 cytokines. Release of IL-8 and GM-CSF was also augmented. IP-10 release was blocked by a PI3K inhibitor and IL-8 by dexamethasone. CONCLUSION: Th2 cytokines increase release of inflammatory cytokines in the presence of rhinovirus infection. This increase is independent of effects of virus replication. Inhibition of the PI3K pathway inhibits IP-10 expression.

  19. IP validation in remote microelectronics testing

    Science.gov (United States)

    Osseiran, Adam; Eshraghian, Kamran; Lachowicz, Stefan; Zhao, Xiaoli; Jeffery, Roger; Robins, Michael

    2004-03-01

    This paper presents the test and validation of FPGA based IP using the concept of remote testing. It demonstrates how a virtual tester environment based on a powerful, networked Integrated Circuit testing facility, aimed to complement the emerging Australian microelectronics based research and development, can be employed to perform the tasks beyond the standard IC test. IC testing in production consists in verifying the tested products and eliminating defective parts. Defects could have a number of different causes, including process defects, process migration and IP design and implementation errors. One of the challenges in semiconductor testing is that while current fault models are used to represent likely faults (stuck-at, delay, etc.) in a global context, they do not account for all possible defects. Research in this field keeps growing but the high cost of ATE is preventing a large community from accessing test and verification equipment to validate innovative IP designs. For these reasons a world class networked IC teletest facility has been established in Australia under the support of the Commonwealth government. The facility is based on a state-of-the-art semiconductor tester operating as a virtual centre spanning Australia and accessible internationally. Through a novel approach the teletest network provides virtual access to the tester on which the DUT has previously been placed. The tester software is then accessible as if the designer is sitting next to the tester. This paper presents the approach used to test and validate FPGA based IPs using this remote test approach.

  20. Effects of flavonoid mixtures on the transport of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) through Caco-2 monolayers: an in vitro and a kinetic modeling approach to predict the combined effects on transporter inhibition

    NARCIS (Netherlands)

    Schutte, M.E.; Boersma, M.G.; Verhallen, D.A.M.; Groten, J.P.; Rietjens, I.M.C.M.

    2008-01-01

    This study describes and kinetically models the effect of flavonoid mixtures on PhIP transport through Caco-2 monolayers. Previously it was shown that quercetin, luteolin, naringenin and myricetin increase the apical to basolateral PhIP transport in Caco-2 monolayers. In this study, apigenin was sho

  1. Qos and Voice Over IP

    Directory of Open Access Journals (Sweden)

    Adrian GHENCEA

    Full Text Available As Voice over Internet Protocol (VoIP technology matures, companies are increasingly adopting it to cut costs, improve efficiency and enhance customer service. Using the Internet as an existing network for integrating data and telecom systems through intelligent VoIP, a range of benefits results: lower long distance costs, cost cuts in cabling processes and more flexible telephony management. However, as voice over IP services grow in popularity, major threats arise: this rapid growth leads to traffic congestion, security is jeopardizedand the poor quality of calls affects communication. The objective of this article is to present all the elements that can affect voicequality in a VoIP network and to provide methods for solving them. A detailed analysis to minimize the impact of implementation of QoS will be made, and at the end solutions to management strategies will be proposed.

  2. Hardware IP security and trust

    CERN Document Server

    Bhunia, Swarup; Tehranipoor, Mark

    2017-01-01

    This book provides an overview of current Intellectual Property (IP) based System-on-Chip (SoC) design methodology and highlights how security of IP can be compromised at various stages in the overall SoC design-fabrication-deployment cycle. Readers will gain a comprehensive understanding of the security vulnerabilities of different types of IPs. This book would enable readers to overcome these vulnerabilities through an efficient combination of proactive countermeasures and design-for-security solutions, as well as a wide variety of IP security and trust assessment and validation techniques. This book serves as a single-source of reference for system designers and practitioners for designing secure, reliable and trustworthy SoCs.

  3. Telomere reprogramming and maintenance in porcine iPS cells.

    Science.gov (United States)

    Ji, Guangzhen; Ruan, Weimin; Liu, Kai; Wang, Fang; Sakellariou, Despoina; Chen, Jijun; Yang, Yang; Okuka, Maja; Han, Jianyong; Liu, Zhonghua; Lai, Liangxue; Gagos, Sarantis; Xiao, Lei; Deng, Hongkui; Li, Ning; Liu, Lin

    2013-01-01

    Telomere reprogramming and silencing of exogenous genes have been demonstrated in mouse and human induced pluripotent stem cells (iPS cells). Pigs have the potential to provide xenotransplant for humans, and to model and test human diseases. We investigated the telomere length and maintenance in porcine iPS cells generated and cultured under various conditions. Telomere lengths vary among different porcine iPS cell lines, some with telomere elongation and maintenance, and others telomere shortening. Porcine iPS cells with sufficient telomere length maintenance show the ability to differentiate in vivo by teratoma formation test. IPS cells with short or dysfunctional telomeres exhibit reduced ability to form teratomas. Moreover, insufficient telomerase and incomplete telomere reprogramming and/or maintenance link to sustained activation of exogenous genes in porcine iPS cells. In contrast, porcine iPS cells with reduced expression of exogenous genes or partial exogene silencing exhibit insufficient activation of endogenous pluripotent genes and telomerase genes, accompanied by telomere shortening with increasing passages. Moreover, telomere doublets, telomere sister chromatid exchanges and t-circles that presumably are involved in telomere lengthening by recombination also are found in porcine iPS cells. These data suggest that both telomerase-dependent and telomerase-independent mechanisms are involved in telomere reprogramming during induction and passages of porcine iPS cells, but these are insufficient, resulting in increased telomere damage and shortening, and chromosomal instability. Active exogenes might compensate for insufficient activation of endogenous genes and incomplete telomere reprogramming and maintenance of porcine iPS cells. Further understanding of telomere reprogramming and maintenance may help improve the quality of porcine iPS cells.

  4. Protection against cancer by dietary IP6 and inositol.

    Science.gov (United States)

    Vucenik, Ivana; Shamsuddin, AbulKalam M

    2006-01-01

    Inositol hexaphosphate (IP(6)) is a naturally occurring polyphosphorylated carbohydrate, abundantly present in many plant sources and in certain high-fiber diets, such as cereals and legumes. In addition to being found in plants, IP(6) is contained in almost all mammalian cells, although in much smaller amounts, where it is important in regulating vital cellular functions such as signal transduction, cell proliferation, and differentiation. For a long time IP(6) has been recognized as a natural antioxidant. Recently IP(6) has received much attention for its role in cancer prevention and control of experimental tumor growth, progression, and metastasis. In addition, IP(6) possesses other significant benefits for human health, such as the ability to enhance immune system, prevent pathological calcification and kidney stone formation, lower elevated serum cholesterol, and reduce pathological platelet activity. In this review we show the efficacy and discuss some of the molecular mechanisms that govern the action of this dietary agent. Exogenously administered IP(6) is rapidly taken up into cells and dephosphorylated to lower inositol phosphates, which further affect signal transduction pathways resulting in cell cycle arrest. A striking anticancer action of IP(6) was demonstrated in different experimental models. In addition to reducing cell proliferation, IP(6) also induces differentiation of malignant cells. Enhanced immunity and antioxidant properties also contribute to tumor cell destruction. Preliminary studies in humans show that IP(6) and inositol, the precursor molecule of IP(6), appear to enhance the anticancer effect of conventional chemotherapy, control cancer metastases, and improve quality of life. Because it is abundantly present in regular diet, efficiently absorbed from the gastrointestinal tract, and safe, IP(6) + inositol holds great promise in our strategies for cancer prevention and therapy. There is clearly enough evidence to justify the

  5. VoIP Forensic Analyzer

    Directory of Open Access Journals (Sweden)

    M Mohemmed Sha

    2016-01-01

    Full Text Available People have been utilizing Voice over Internet Protocol (VoIP in most of the conventional communication facilities which has been of assistance in the enormous attenuation of operating costs, as well as the promotion of next- generation communication services-based IP. As an intimidating upshot, cyber criminals have correspondingly started interjecting the environment and creating new challenges for the law enforcement system in any Country. This paper presents an idea of a framework for the forensic analysis of the VoIP traffic over the network. This forensic activity includes spotting and scrutinizing the network patterns of VoIP-SIP stream, which is used to initiate a session for the communication, and regenerate the content from VoIP-RTP stream, which is employed to convey the data. Proposed network forensic investigation framework also accentuates on developing an efficient packet restructuring algorithm for tracing the depraved users involved in a conversation. Network forensics is the basis of proposed work, and performs packet level surveillance of VoIP followed by reconstruction of original malicious content or network session between users for their prosecution in the court.

  6. Complete genome sequence of Rhodothermus marinus type strain (R-10T)

    Energy Technology Data Exchange (ETDEWEB)

    Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Gronow, Sabine [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Lapidus, Alla L. [U.S. Department of Energy, Joint Genome Institute; Ivanova, N [U.S. Department of Energy, Joint Genome Institute; Copeland, A [U.S. Department of Energy, Joint Genome Institute; Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Glavina Del Rio, Tijana [U.S. Department of Energy, Joint Genome Institute; Chen, Feng [U.S. Department of Energy, Joint Genome Institute; Tice, Hope [U.S. Department of Energy, Joint Genome Institute; Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Cheng, Jan-Fang [U.S. Department of Energy, Joint Genome Institute; Sims, David [Los Alamos National Laboratory (LANL); Meincke, Linda [Los Alamos National Laboratory (LANL); Bruce, David [Los Alamos National Laboratory (LANL); Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Han, Cliff [Los Alamos National Laboratory (LANL); Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Ovchinnikova, Galina [U.S. Department of Energy, Joint Genome Institute; Pati, Amrita [U.S. Department of Energy, Joint Genome Institute; Mavromatis, K [U.S. Department of Energy, Joint Genome Institute; Mikhailova, Natalia [U.S. Department of Energy, Joint Genome Institute; Chen, Amy [U.S. Department of Energy, Joint Genome Institute; Palaniappan, Krishna [U.S. Department of Energy, Joint Genome Institute; Land, Miriam L [ORNL; Hauser, Loren John [ORNL; Chang, Yun-Juan [ORNL; Jeffries, Cynthia [Oak Ridge National Laboratory (ORNL); Rohde, Manfred [HZI - Helmholtz Centre for Infection Research, Braunschweig, Germany; Sproer, Cathrin [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Goker, Markus [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Bristow, James [U.S. Department of Energy, Joint Genome Institute; Eisen, Jonathan [U.S. Department of Energy, Joint Genome Institute; Markowitz, Victor [U.S. Department of Energy, Joint Genome Institute; Hugenholtz, Philip [U.S. Department of Energy, Joint Genome Institute; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute; Klenk, Hans-Peter [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Chain, Patrick S. G. [Lawrence Livermore National Laboratory (LLNL)

    2009-01-01

    Rhodothermus marinus Alfredsson et al. 1995 is the type species of the genus and is of phylogenetic interest because the Rhodothermaceae represent the deepest lineage in the phylum Bacteroidetes. R. marinus R-10T is a Gram-negative, non-motile, non-spore-forming bacterium isolated from marine hot springs off the coast of Iceland. Strain R-10T is strictly aerobic and requires slightly halophilic conditions for growth. Here we describe the features of this organism, together with the complete genome sequence, and annotation. This is the first complete genome sequence of the genus Rhodothermus, and only the second sequence from members of the family Rhodothermaceae. The 3,386,737 bp genome (including a 125 kb plasmid) with its 2914 protein-coding and 48 RNA genes is part of the Genomic Encyclopedia of Bacteria and Archaea project.

  7. LTE/LTE-Advanced R10标准化进展

    Institute of Scientific and Technical Information of China (English)

    严文发

    2011-01-01

    介绍了LTE/LTE-Advanced标准发展概况,详细分析了近期LTE-Advanced R10版本技术标准化情况及可行性阶段技术的进展,同时展望了LTE-Advanced R11版本的发展方向,最后分析了TD-LTE现网测试情况.

  8. iPS cells and personalized medicine

    OpenAIRE

    2014-01-01

    iPS cells and personalized medicine Personalized medicine uses patient's genotype and/or level of gene expression for selection of medication, therapy or preventative measures. One of the best approaches for developing patient specific therapy is to use patient specific cells as in vitro drug testing model. Furthermore, patent specific cells in vitro enriched and modified can be used for autologous cell transplantation for treating numerous degenerative conditions.

  9. A decentralized software bus based on IP multicas ting

    Science.gov (United States)

    Callahan, John R.; Montgomery, Todd

    1995-01-01

    We describe decentralized reconfigurable implementation of a conference management system based on the low-level Internet Protocol (IP) multicasting protocol. IP multicasting allows low-cost, world-wide, two-way transmission of data between large numbers of conferencing participants through the Multicasting Backbone (MBone). Each conference is structured as a software bus -- a messaging system that provides a run-time interconnection model that acts as a separate agent (i.e., the bus) for routing, queuing, and delivering messages between distributed programs. Unlike the client-server interconnection model, the software bus model provides a level of indirection that enhances the flexibility and reconfigurability of a distributed system. Current software bus implementations like POLYLITH, however, rely on a centralized bus process and point-to-point protocols (i.e., TCP/IP) to route, queue, and deliver messages. We implement a software bus called the MULTIBUS that relies on a separate process only for routing and uses a reliable IP multicasting protocol for delivery of messages. The use of multicasting means that interconnections are independent of IP machine addresses. This approach allows reconfiguration of bus participants during system execution without notifying other participants of new IP addresses. The use of IP multicasting also permits an economy of scale in the number of participants. We describe the MULITIBUS protocol elements and show how our implementation performs better than centralized bus implementations.

  10. Anti-angiogenic activity of inositol hexaphosphate (IP6).

    Science.gov (United States)

    Vucenik, Ivana; Passaniti, Antonino; Vitolo, Michele I; Tantivejkul, Kwanchanit; Eggleton, Paul; Shamsuddin, Abulkalam M

    2004-11-01

    A significant anticancer activity of the naturally occurring carbohydrate inositol hexaphosphate (IP(6)) has been reported against numerous cancer models. Since tumors require angiogenesis for growth and metastasis, we hypothesize that IP(6) reduces tumor growth by inhibiting angiogenesis. Because angiogenesis depends on the interaction between endothelial and tumor cells, we investigated the effect of IP(6) on both. IP(6) inhibited the proliferation and induced the differentiation of endothelial cells in vitro; the growth of bovine aortic endothelial cells (BAECs) evaluated by MTT proliferation assay was inhibited in a dose-dependent manner (IC(50) = 0.74 mM). The combination of IP(6) and vasostatin, a calreticulin fragment with anti-angiogenic activity, was synergistically superior in growth inhibition than either compound. IP(6) inhibited human umbilical vein endothelial cell (HUVEC) tube formation (in vitro capillary differentiation) on a reconstituted extracellular matrix, Matrigel, and disrupted pre-formed tubes. IP(6) significantly reduced basic fibroblast growth factor (bFGF)-induced vessel formation (P < 0.01) in vivo in Matrigel plug assay. Exposure of HepG2, a human hepatoma cell line, to IP(6) for 8 h, resulted in a dose-dependent decrease in the mRNA levels of vascular endothelial growth factor (VEGF), as assessed by RT-PCR. IP(6) treatment of HepG2 cells for 24 h also significantly reduced the VEGF protein levels in conditioned medium, in a concentration-dependent manner (P = 0.012). Thus, IP(6) has an inhibitory effect on induced angiogenesis.

  11. Mobility management in mobile IP

    Science.gov (United States)

    Medidi, Sirisha; Golshani, Forouzan

    2002-07-01

    There is an emerging interest in integrating mobile wireless communication with the Internet based on the Ipv6 technology. Many issues introduced by the mobility of users arise when such an integration is attempted. This paper addresses the problem of mobility management, i.e., that of tracking the current IP addresses of mobile terminals and sustaining active IP connections as mobiles move. The paper presents some architectural and mobility management options for integrating wireless access to the Internet. We then present performance results for Mobile IPv4, route optimization and Mobile IPv6.

  12. Security Risks in IP Telephony

    Directory of Open Access Journals (Sweden)

    Filip Rezac

    2010-01-01

    Full Text Available This paper deals with VoIP communication security and various techniques of VoIP attacks. We divided these threats in several categories according to their specific behaviour and their impact on the affected system. We also tried to find effective methods to prevent or mitigate these attacks. We focused our work on Spam over Internet Telephony (SPIT as a real threat for the future. We have developed both a tool generating SPIT attacks and AntiSPIT tool defending communication systems against SPIT attacks. AntiSPIT represents an effective protection based on statistical blacklist and works without participation of the called party which is a significant advantage.

  13. Combining miR10b-targeted nanotherapy with low-dose doxorubicin elicits durable regressions of metastatic breast cancer

    Science.gov (United States)

    Yoo, Byunghee; Kavishwar, Amol; Ross, Alana; Wang, Ping; Tabassum, Doris P.; Polyak, Kornelia; Barteneva, Natalia; Petkova, Victoria; Pantazopoulos, Pamela; Tena, Aseda; Moore, Anna; Medarova, Zdravka

    2015-01-01

    The therapeutic promise of microRNA in cancer has yet to be realized. In this study, we identified and therapeutically exploited a new role for miR-10b at the metastatic site, which links its overexpression to tumor cell viability and proliferation. In the protocol developed, we combined a miR-10b-inhibitory nanodrug with low-dose anthracycline to achieve complete durable regressions of metastatic disease in a murine model of metastatic breast cancer. Mechanistic investigations suggested a potent anti-proliferative, pro-apoptotic effect of the nanodrug in the metastatic cells, potentiated by a cell-cycle arrest produced by administration of the low-dose anthracycline. miR-10b was overexpressed specifically in cells with high metastatic potential, suggesting a role for this miRNA as a metastasis-specific therapeutic target. Taken together, our results implied the existence of pathways that regulate the viability and proliferation of tumor cells only after they have acquired the ability to grow at distant metastatic sites. As illustrated by miR-10b targeting, such metastasis-dependent apoptotic pathways would offer attractive targets for further therapeutic exploration. PMID:26359455

  14. A brief review of the reflood closure package optimization efforts performed within TRAC 5.4.25R10

    Energy Technology Data Exchange (ETDEWEB)

    Pimentel, D.A.; Nelson, R.A.

    1997-10-01

    This report summarizes the implementation of tools within Version 5.4.25R10 of the Transient Reactor Analysis Code (TRAC); this implementation allows the semiautomated optimization of the reflood constitutive package. The tools included a software package external to TRAC that used a line search method to minimize a generic function value given the function`s partial derivative vector with respect to a set of closure coefficients used within TRAC`s reflood model. Within TRAC, the generic function was a normalized penalty function dependent on time averaged calculated values of vapor temperature, vapor void fraction, wall to a fluid heat transfer rate (or wall temperature), and the respective steady state data. The penalty function was implemented only for a one dimensional vessel configuration because the available reflood data were taken primarily from postcritical heat flux tube experiments.

  15. PENGARUH PENGGUNAAN MODEL COOPERATIVE LEARNING TIPE GROUP INVESTIGATION (GI TERHADAP HASIL BELAJAR IPS TERPADU KELAS VIII SEMESTER GENAP SMPYPI 1 BANDAR MATARAM LAMPUNG TENGAH T.P 2015/2016

    Directory of Open Access Journals (Sweden)

    Desi Fatmawati Maryatun

    2016-05-01

    Full Text Available Metode cooperative learning tipe group investigation merupakan model pembelajaran kooperatif yang dapat melibatkan peserta didik secara aktif dalam kegiatan pembelajaran mulai dari merencanakan topik-topik yang akan dipelajari, bagaimana melaksanakan investigasinya, hingga melakukan presentasi kelompok dan evaluasi. Tujuan penelitian ini adalah untuk mengetahui adanya pengaruh penggunaan model pembelajaran cooperative learning tipe Group Investigation terhadap hasil belajar IPS Terpadu peserta didik kelas VIII semester genap SMP YPI 1 Bandar Mataram  Lampung Tengah tahun pelajaran 2015/2016. Hipotesis yang penulis ajukan adalah “Ada pengaruh yang positif pada model pembelajaran cooperative learning tipe Group Investigation terhadap hasil belajar IPS Terpadu peserta didik kelas VIII semester genap SMP YPI 1 Bandar Mataram  Lampung Tengah tahun pelajaran 2015/2016. Populasi dalam penelitian ini adalah siswa kelas VIII SMP YPI 1 Bandar Mataram Lampung Tengah Tahun Pelajaran 2015/2016 yaitu berjumlah 48 orang siswa dan diantaranya terdiri dari 2 kelas. Dan yang menjadi sampel dalam penelitian ini adalah kelas VIIIa dan VIIIb. Kelas VIIIa sebagai kelas eksperimen dan kelas VIIIb sebagai kelas control, sampel diambil menggunakan teknik purposive sampling, Eksperimen dilaksanakan pada siswa kelas VIIIa Semester Genap SMP YPI 1 Bandar Mataram Lampung Tengah Tahun Pelajaran 2015/2016 yang berjumlah 24 peserta didik. Data penelitian ini dikumpulkan dengan menggunakan  metode observasi, wawancara, dokumentasi, dan tes. Sedangkan untuk mengetahui tingkat validitas dan reliabilitas penulis menggunakan rumus K-R 20. Kemudian untuk menguji/membuktikan hipotesis digunakan rumus Regresi Linier Sederhana yaitu  Ŷ = a + bx. Nilai Ŷ = 73,33+ 0,5 X yang dilanjutkan dengan rumus thitung > ttabel.pada daftar signifikan 5% yaitu 4 > 1,72  dan pada taraf signifikan 1% yaitu 4 > 2,52. Dengan demikian hipotesisnya diterima karena ada pengaruh yang positif

  16. IP3 receptors regulate vascular smooth muscle contractility and hypertension

    Science.gov (United States)

    Lin, Qingsong; Zhao, Guiling; Fang, Xi; Peng, Xiaohong; Tang, Huayuan; Wang, Hong; Jing, Ran; Liu, Jie; Ouyang, Kunfu

    2016-01-01

    Inositol 1, 4, 5-trisphosphate receptor–mediated (IP3R-mediated) calcium (Ca2+) release has been proposed to play an important role in regulating vascular smooth muscle cell (VSMC) contraction for decades. However, whether and how IP3R regulates blood pressure in vivo remains unclear. To address these questions, we have generated a smooth muscle–specific IP3R triple-knockout (smTKO) mouse model using a tamoxifen-inducible system. In this study, the role of IP3R-mediated Ca2+ release in adult VSMCs on aortic vascular contractility and blood pressure was assessed following tamoxifen induction. We demonstrated that deletion of IP3Rs significantly reduced aortic contractile responses to vasoconstrictors, including phenylephrine, U46619, serotonin, and endothelin 1. Deletion of IP3Rs also dramatically reduced the phosphorylation of MLC20 and MYPT1 induced by U46619. Furthermore, although the basal blood pressure of smTKO mice remained similar to that of wild-type controls, the increase in systolic blood pressure upon chronic infusion of angiotensin II was significantly attenuated in smTKO mice. Taken together, our results demonstrate an important role for IP3R-mediated Ca2+ release in VSMCs in regulating vascular contractility and hypertension.

  17. Continuously Connected With Mobile IP

    Science.gov (United States)

    2002-01-01

    Cisco Systems developed Cisco Mobile Networks, making IP devices mobile. With this innovation, a Cisco router and its connected IP devices can roam across network boundaries and connection types. Because a mobile user is able to keep the same IP address while roaming, a live IP connection can be maintained without interruption. Glenn Research Center jointly tested the technology with Cisco, and is working to use it on low-earth-orbiting research craft. With Cisco's Mobile Networks functionality now available in Cisco IOS Software release 12.2(4)T, the commercial advantages and benefits are numerous. The technology can be applied to public safety, military/homeland security, emergency management services, railroad and shipping systems, and the automotive industry. It will allow ambulances, police, firemen, and the U.S. Coast Guard to stay connected to their networks while on the move. In the wireless battlefield, the technology will provide rapid infrastructure deployment for U.S. national defense. Airline, train, and cruise passengers utilizing Cisco Mobile Networks can fly all around the world with a continuous Internet connection. Cisco IOS(R) Software is a registered trademark of Cisco Systems.

  18. Introduction to IP address management

    CERN Document Server

    Rooney, Tim

    2010-01-01

    "The book begins with a basic overview of IP networking, followed by chapters describing each of the three core IPAM technologies: IPv4 and IPv6 addressing, DHCP, and DNS. The next three chapters describe IPAM management techniques and practice, followed by chapters on IPv4-IPv6 co-existence, security and the IPAM business case"--

  19. Controlling IP Falsifying Using Realistic Simulation

    Directory of Open Access Journals (Sweden)

    Govindavaram Madhusri

    2013-07-01

    Full Text Available The study of Internet-scale events such as worm proliferation, distributed denial-of-service attacks (DDoS,flash crowds, routing volatilities, and DNS attacks depend on the formation of all the networks thatgenerate or forward valid and malevolent traffic,The Distributed Denial of Services (DDoS attack is aserious threat to the valid use of the Internet. Forestalling mechanisms are disappointed by the ability ofattackers to steal, or spoof, the source addresses in IP packets. IP falsifying is still widespread in networkscanning and investigates, as well as denial of service floods.IDPFs can limit the falsifying capability ofattackers. Moreover, it works on a small number of candidate networks easily traceable, thus simplifyingthe reactive IP trace back process. However, this technique does not allow large number of networks,which is a common misapprehension for those unfamiliar with the practice. Current network simulatorscannot be used to study Internet-scale events. They are general-purpose, packet-level simulators thatreproduce too many details of network communication, which limits scalability. We propose to develop adistributed Internet simulator, with the following novel features. It will provide a built-in Internet model,including the topology, routing, link bandwidths and delays, Instead of being a general-purpose simulator,it will provide a common simulation core for traffic generation and message passing, on top of which wewill build separate modules that customize messages and level of simulation details for the event of interest.Customization modules will ensure that all and only the relevant details of the event of interest aresimulated, cutting down the simulation time. We will also provide an interface for new modulespecification, and for existing module modification, this will bring the Internet event simulation at thefingertips of all interested researchers. The simulator will promote research in worm detection and defense,IP

  20. Chemoenzymatic asymmetric total syntheses of antitumor agents (3R,9R,10R)- and (3S,9R,10R)-Panaxytriol and (R)- and (S)-Falcarinol from Panax ginseng using an enantioconvergent enzyme-triggered cascade reaction.

    Science.gov (United States)

    Mayer, Sandra F; Steinreiber, Andreas; Orru, Romano V A; Faber, Kurt

    2002-12-27

    Total asymmetric synthesis of two components of Panax ginseng showing antitumor activity, i.e., (3R,9R,10R)- and (3S,9R,10R)-Panaxytriol and of both enantiomers of Falcarinol was accomplished. Due to the fact that the synthetic strategy was based on enantioconvergent biotransformations, the occurrence of any undesired stereoisomer was entirely avoided. The absolute configuration of naturally occurring Panaxytriol was confirmed to be (3R,9R,10R) on the basis of optical rotation values. It was shown that enzyme-triggered cascade reactions represent a valuable tool for the synthesis of natural products.

  1. On using Mobile IP Protocols

    Directory of Open Access Journals (Sweden)

    Fayza A. Nada

    2006-01-01

    Full Text Available The recent advances in wireless communication technology and the unprecedented growth of the Internet have paved the way for wireless networking and IP mobility. Mobile Internet protocol has been designed within the IETF to support the mobility of users who wish to connect to the Internet and maintain communications as they move from place to place. This study describes and summarizes the current Internet draft for mobile IP (MIPv4 with its major components: agent discovery, registration and tunneling. In addition, we outline the available encapsulation techniques and route optimization procedure. In the end, we describe the design of the new protocol for transparent routing of IPv6 packets to mobile IPv6 nodes operating in the Internet.

  2. Security in VoIP

    Directory of Open Access Journals (Sweden)

    Floriana GEREA

    2012-04-01

    Full Text Available VoIP relies on packet switching, similar to the way that e-mails are sent over the Internet. The technology breaks down a voice call into bite-size information packets. Instead of keeping the switch open all the time, the information is sent and received as needed, allowing excess line capacity to be used to carry other data. When the voice data arrives at its destination, it's reassembled into a voice call. As voice over IP services grow in popularity, the potential for viruses, worms and other security threats aimed at the technology also will grow. The current paper's purpose is presenting several security solutions and applying them to integrated systems at an economical and social level.

  3. Internetworking with TCP/IP

    CERN Document Server

    Comer, Douglas E

    2014-01-01

    An internationally best-selling, conceptual introduction to the TCP/IP protocols and Internetworking, this book interweaves a clear discussion of fundamentals and scientific principles with details and examples drawn from the latest technologies. Leading author Douglas Comer covers layering and packet formats for all the Internet protocols, includingTCP, IPv4, IPv6, DHCP, and DNS. In addition, the text explains new trends in Internet systems, including packet classification, Software Defined Networking (SDN), and mesh protocols used in The Internet of Things. The text is appropriate for individuals interested in learning more about TCP/IP protocols, Internet architecture, and current networking technologies, as well as engineers who build network systems. It is suitable for junior to graduate-level courses in Computer Networks, Data Networks, Network Protocols, and Internetworking.

  4. OSI and TCP/IP

    Science.gov (United States)

    Randolph, Lynwood P.

    1994-01-01

    The Open Systems Interconnection Transmission Control Protocol/Internet Protocol (OSI TCP/IP) and the Government Open Systems Interconnection Profile (GOSIP) are compared and described in terms of Federal internetworking. The organization and functions of the Federal Internetworking Requirements Panel (FIRP) are discussed and the panel's conclusions and recommendations with respect to the standards and implementation of the National Information Infrastructure (NII) are presented.

  5. IP Rights and Technological Platforms

    OpenAIRE

    Robert P. Merges

    2008-01-01

    This paper is about intellectual property rights (IPRs) and platform technologies. After a brief introduction explaining some basics of networks, standards and platforms, I turn to three policy issues. The first is the role of IP in what might be termed platform policies, the decisions by courts and regulators concerning whether and how to promote multi-party access to important digital platforms such as media player hardware, cell phones, PCs, and the like. I argue that for the most part the...

  6. Comparison of gating dynamics of different IP3R channels with immune algorithm searching for channel parameter distributions

    Science.gov (United States)

    Cai, Xiuhong; Li, Xiang; Qi, Hong; Wei, Fang; Chen, Jianyong; Shuai, Jianwei

    2016-10-01

    The gating properties of the inositol 1, 4, 5-trisphosphate (IP3) receptor (IP3R) are determined by the binding and unbinding capability of Ca2+ ions and IP3 messengers. With the patch clamp experiments, the stationary properties have been discussed for Xenopus oocyte type-1 IP3R (Oo-IP3R1), type-3 IP3R (Oo-IP3R3) and Spodoptera frugiperda IP3R (Sf-IP3R). In this paper, in order to provide insights about the relation between the observed gating characteristics and the gating parameters in different IP3Rs, we apply the immune algorithm to fit the parameters of a modified DeYoung-Keizer model. By comparing the fitting parameter distributions of three IP3Rs, we suggest that the three types of IP3Rs have the similar open sensitivity in responding to IP3. The Oo-IP3R3 channel is easy to open in responding to low Ca2+ concentration, while Sf-IP3R channel is easily inhibited in responding to high Ca2+ concentration. We also show that the IP3 binding rate is not a sensitive parameter for stationary gating dynamics for three IP3Rs, but the inhibitory Ca2+ binding/unbinding rates are sensitive parameters for gating dynamics for both Oo-IP3R1 and Oo-IP3R3 channels. Such differences may be important in generating the spatially and temporally complex Ca2+ oscillations in cells. Our study also demonstrates that the immune algorithm can be applied for model parameter searching in biological systems.

  7. Principle analysis of IP wavelength router

    Institute of Scientific and Technical Information of China (English)

    王勇; 殷洪玺; 徐安士; 吴德明

    2001-01-01

    Combining IP with WDM is an attractive direction for research. WDM will play an important role in IP network in future. Now, an urgent problem is how to introduce wavelength routing in an IP network. We solve this problem by designing IP wavelength router, implementing DPDP (default path and dedicated path) method. We prove the reasonableness and feasibility of this design by a principle experiment. A lot of problems related to this design are also discussed.

  8. IP and Competitiveness of Chinese Telecom Firms

    Institute of Scientific and Technical Information of China (English)

    ZENG Jian-qiu

    2004-01-01

    Based on the influence, this paper discusses the perspectives of IP technology. It illustrates that IP is changing the service structure of China Telecom and IP is striking traditional telecom networks and changing the competitive structure of telecom markets. IP is one of core technologies in future telecom networks and there is an opportunity for Chinese telecom firms to quicken their network innovation and improve the competitiveness for their sustainable development.

  9. A Distributed IP-Based Telecommunication System using SIP

    Directory of Open Access Journals (Sweden)

    Carlton Andre Thompson

    2013-11-01

    Full Text Available Voice over Internet Protocol (VoIP technologies are integral to modern telecommunications because oftheir advanced features, flexibility, and economicbenefits. Internet Service Providers initially promotedthese technologies by providing low cost local andinternational calling. At present, there is also agreatdeal of interest in using IP-based technologies toreplace traditional small and large office telephonesystems that use traditional PBX’s (Private BrancheXchange. Unfortunately, the large majority of theemerging VoIP based office telephone systems have followed the centralized design of traditional publicand private telephone systems in which all the intelligence in the system is at the core, with quite expensivehardware and software components and appropriate redundancy for adequate levels of reliability. In thispaper, it is argued that a centralized model for anIP-based telecommunications system fails to exploit thefull capabilities of Internet-inspired communications and that, very simple, inexpensive, elegant andflexible solutions are possible by deliberately avoiding the centralized approach. This paper describes thedesign, philosophy and implementation of a prototype for a fully distributed IP-based TelecommunicationSystem (IPTS that provides the essential feature set for office and home telecommunications, including IP-based long-distance and local calling, and with thesupport for video as well as data and text. Theprototype system was implemented with an Internet-inspired distributed design using open source software,with appropriate customizations and configurations.

  10. Towards Multi-Stage Intrusion Detection using IP Flow Records

    Directory of Open Access Journals (Sweden)

    Muhammad Fahad Umer

    2016-10-01

    Full Text Available Traditional network-based intrusion detection sys-tems using deep packet inspection are not feasible for modern high-speed networks due to slow processing and inability to read encrypted packet content. As an alternative to packet-based intrusion detection, researchers have focused on flow-based intrusion detection techniques. Flow-based intrusion detection systems analyze IP flow records for attack detection. IP flow records contain summarized traffic information. However, flow data is very large in high-speed networks and cannot be processed in real-time by the intrusion detection system. In this paper, an efficient multi-stage model for intrusion detection using IP flows records is proposed. The first stage in the model classifies the traffic as normal or malicious. The malicious flows are further analyzed by a second stage. The second stage associates an attack type with malicious IP flows. The proposed multi-stage model is efficient because the majority of IP flows are discarded in the first stage and only malicious flows are examined in detail. We also describe the implementation of our model using machine learning techniques.

  11. Challenges Regarding IP Core Functional Reliability

    Science.gov (United States)

    Berg, Melanie D.; LaBel, Kenneth A.

    2017-01-01

    For many years, intellectual property (IP) cores have been incorporated into field programmable gate array (FPGA) and application specific integrated circuit (ASIC) design flows. However, the usage of large complex IP cores were limited within products that required a high level of reliability. This is no longer the case. IP core insertion has become mainstream including their use in highly reliable products. Due to limited visibility and control, challenges exist when using IP cores and subsequently compromise product reliability. We discuss challenges and suggest potential solutions to critical application IP insertion.

  12. Comparative study of myocytes from normal and mdx mice iPS cells.

    Science.gov (United States)

    Chen, Fei; Cao, Jiqing; Liu, Qiang; Qin, Jie; Kong, Jie; Wang, Yanyun; Li, Yaqin; Geng, Jia; Li, Qiuling; Yang, Liqing; Xiang, Andy Peng; Zhang, Cheng

    2012-02-01

    Recently, induced pluripotent stem cells (iPS cells) have been derived from various techniques and show great potential for therapy of human diseases. Furthermore, the iPS technique can be used to provide cell models to explore pathological mechanisms of many human diseases in vitro, such as Duchenne muscular dystrophy (DMD), which is a severe recessive X-linked form of muscular dystrophy without effective treatment. In this study, we try to determine whether there are different characteristics of myocytes from mdx iPS cells and C57BL/10 iPS cells. Our results showed that both of mdx and C57BL/10 cells could be induced into iPS cells in vitro, whereas colony-forming ability of mdx iPS cells was much weaker than that of C57BL/10 iPS cells. Meanwhile, mdx iPS cells could be induced to differentiate into myocytes, whereas their differentiation efficiency was much lower than that of C57BL/10 iPS cells. And, the number of apoptotic cells in differentiated myocytes from mdx iPS cells was significantly higher than that from C57BL/10 iPS cells. More importantly, treatment of a pan-caspase inhibitor (Z-VAD) produced a significant decrease in apoptotic cells. This study might add some insight to the biology study of dystrophin gene.

  13. TCP/IP Network Administration

    CERN Document Server

    Hunt, Craig

    2002-01-01

    This complete guide to setting up and running a TCP/IP network is essential for network administrators, and invaluable for users of home systems that access the Internet. The book starts with the fundamentals -- what protocols do and how they work, how addresses and routing are used to move data through the network, how to set up your network connection -- and then covers, in detail, everything you need to know to exchange information via the Internet. Included are discussions on advanced routing protocols (RIPv2, OSPF, and BGP) and the gated software package that implements them, a tutorial

  14. Live Video and IP-TV

    Science.gov (United States)

    Merani, Maria Luisa; Saladino, Daniela

    This Chapter aims at providing a comprehensive insight into the most recent advances in the field of P2P architectures for video broadcasting, focusing on live video streaming P2P live video streaming . After introducing a classification of P2P video solutions, the first part of the Chapter provides an overview of the most interesting P2P IP-TV P2P IP-TV systems currently available over the Internet. It also concentrates on the process of data diffusion within the P2P overlay and complements this view with some measurements that highlight the most salient features of P2P architectures. The second part of the Chapter completes the view, bringing up the modeling efforts to capture the main characteristics and limits of P2P streaming systems, both analytically and numerically. The Chapter is closed by a pristine look at some challenging, open questions, with a specific emphasis on the adoption of network coding in P2P streaming solutions.

  15. Implementasi Sistem IP PBX menggunakan Briker

    Directory of Open Access Journals (Sweden)

    DWI ARYANTA

    2017-06-01

    Full Text Available Abstrak VoIP (Voice over Internet Protocol adalah komunikasi suara jarak jauh yang digunakan melalui jaringan IP. Pada penelitian ini dirancang sistem IP PBX dengan menggunakan teknologi berbasis VoIP. IP PBX adalah perangkat switching komunikasi telepon dan data berbasis teknologi Internet Protocol (IP yang mengendalikan ekstension telepon analog maupun ekstension IP Phone. Software VirtualBox digunakan dengan tujuan agar lebih memudahkan dalam sistem pengoperasian Linux yang dimana program untuk membuat IP PBX adalah menggunakan Briker yang bekerja pada Operating System Linux 2.6. Setelah proses penginstalan Briker pada Virtualbox dilakukan implementasi jaringan IP PBX. Setelah mengimplementasikan jaringan IP PBX sesuai dengan topologi, kemudian melakukan pengujian success call rate dan analisis Quality of Service (QoS. Pengukuran QoS menggunakan parameter jitter, delay, dan packet loss yang dihasilkan dalam sistem IP PBX ini. Nilai jitter sesama user Briker (baik pada smartphone maupun komputer mempunyai rata-rata berada pada nilai 16,77 ms. Sedangkan nilai packetloss yang didapat pada saat terdapat pada saat user 1 sebagai pemanggil telepon adalah 0%. Sedangkan persentase packet loss pada saat user 1 sebagai penerima telepon adalah 0,01%. Nilai delay pada saat berkomunikasi antar user berada pada 11,75 ms. Secara keseluruhan nilai yang didapatkan melalui penelitian ini, dimana hasil pengujian parameter-parameter QOS sesuai dengan standar yang telah direkomendasikan oleh ITU dan didapatkan nilai QoS dengan hasil “baik”. Kata Kunci : Briker, VoIP, QoS, IP PBX, Smartphone. Abstract VoIP (Voice over Internet Protocol is a long-distance voice communications over IP networks are used. In this study, IP PBX systems designed using VoIP -based technologies. IP PBX is a telephone switching device and data communication technology-based Internet Protocol (IP which controls the analog phone extensions and IP Phone extensions. VirtualBox software is

  16. Vaccines and IP Rights: A Multifaceted Relationship.

    Science.gov (United States)

    Durell, Karen

    2016-01-01

    Just as there are many forms of vaccines and components to vaccines-particular compositions, delivery systems, components, and distribution networks-there are a variety of intellectual property (IP) protections applicable for vaccines. IP rights such as patent, copyright, trademarks, plant breeders' rights, and trade secrets may all be applicable to vaccines. Thus, discussion of IP rights and vaccines should not begin and end with the application of one IP right to a vaccine. The discussion should engage considerations of multiple IP rights applicable to a vaccine and how these can be utilized in an integrated manner in a strategy aimed at supporting the development and distribution of the vaccine. Such an approach to IP rights to vaccines allows for the integrated rights to be considered in light of the justifications for protecting vaccines with IP rights, as well as the issues relating to specific IP rights for vaccines, such as compulsory license regimes, available humanitarian purpose IP credits, etc. To view vaccines as the subject of multiple IP protections involves a refocusing, but the outcome can provide significant benefits for vaccine development and distribution.

  17. Applying a gaming approach to IP strategy.

    Science.gov (United States)

    Gasnier, Arnaud; Vandamme, Luc

    2010-02-01

    Adopting an appropriate IP strategy is an important but complex area, particularly in the pharmaceutical and biotechnology sectors, in which aspects such as regulatory submissions, high competitive activity, and public health and safety information requirements limit the amount of information that can be protected effectively through secrecy. As a result, and considering the existing time limits for patent protection, decisions on how to approach IP in these sectors must be made with knowledge of the options and consequences of IP positioning. Because of the specialized nature of IP, it is necessary to impart knowledge regarding the options and impact of IP to decision-makers, whether at the level of inventors, marketers or strategic business managers. This feature review provides some insight on IP strategy, with a focus on the use of a new 'gaming' approach for transferring the skills and understanding needed to make informed IP-related decisions; the game Patentopolis is discussed as an example of such an approach. Patentopolis involves interactive activities with IP-related business decisions, including the exploitation and enforcement of IP rights, and can be used to gain knowledge on the impact of adopting different IP strategies.

  18. Analysis of Handoff Mechanisms in Mobile IP

    Science.gov (United States)

    Jayaraj, Maria Nadine Simonel; Issac, Biju; Haldar, Manas Kumar

    2011-06-01

    One of the most important challenges in mobile Internet Protocol (IP) is to provide service for a mobile node to maintain its connectivity to network when it moves from one domain to another. IP is responsible for routing packets across network. The first major version of IP is the Internet Protocol version 4 (IPv4). It is one of the dominant protocols relevant to wireless network. Later a newer version of IP called the IPv6 was proposed. Mobile IPv6 is mainly introduced for the purpose of mobility. Mobility management enables network to locate roaming nodes in order to deliver packets and maintain connections with them when moving into new domains. Handoff occurs when a mobile node moves from one network to another. It is a key factor of mobility because a mobile node can trigger several handoffs during a session. This paper briefly explains on mobile IP and its handoff issues, along with the drawbacks of mobile IP.

  19. [iPS cells in dentistry].

    Science.gov (United States)

    Egusa, Hiroshi

    2012-01-01

    The discovery of the induced pluripotent stem (iPS) cell technology, which enables us to produce pluripotent stem cells by introducing a few genetic factors, commands considerable attention in the field of dentistry. These iPS cells may be of particular importance for developing innovative technologies to regenerate missing jaw bones and lost teeth, and there are expectations that several types of tissue stem cells and mucosal cells in the oral area can be used as an ideal iPS cell source. We previously reported that the gingiva, which is often resected during general dental treatments and treated as biomedical waste, is a promising source of iPS cells. In this review, the current trends in iPS cell research in dentistry are outlined, and future aspects of potential applications of the iPS cell technologies to dental treatments will be discussed.

  20. Evaluation and Investigation of the Delay in VoIP Networks

    Directory of Open Access Journals (Sweden)

    V. Janata

    2011-06-01

    Full Text Available The paper is focused mainly on the delay problems, which considerably influence the final quality of connections in VoIP (Voice over IP networks. The paper provides a detailed exploration of the nature and mechanisms of the delay. The main purpose of the investigation was an attempt to formulate a mathematical model of delay in the VoIP network and its subsequent analysis by laboratory data.

  1. ChIP-PIT: Enhancing the Analysis of ChIP-Seq Data Using Convex-Relaxed Pair-Wise Interaction Tensor Decomposition.

    Science.gov (United States)

    Zhu, Lin; Guo, Wei-Li; Deng, Su-Ping; Huang, De-Shuang

    2016-01-01

    In recent years, thanks to the efforts of individual scientists and research consortiums, a huge amount of chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) experimental data have been accumulated. Instead of investigating them independently, several recent studies have convincingly demonstrated that a wealth of scientific insights can be gained by integrative analysis of these ChIP-seq data. However, when used for the purpose of integrative analysis, a serious drawback of current ChIP-seq technique is that it is still expensive and time-consuming to generate ChIP-seq datasets of high standard. Most researchers are therefore unable to obtain complete ChIP-seq data for several TFs in a wide variety of cell lines, which considerably limits the understanding of transcriptional regulation pattern. In this paper, we propose a novel method called ChIP-PIT to overcome the aforementioned limitation. In ChIP-PIT, ChIP-seq data corresponding to a diverse collection of cell types, TFs and genes are fused together using the three-mode pair-wise interaction tensor (PIT) model, and the prediction of unperformed ChIP-seq experimental results is formulated as a tensor completion problem. Computationally, we propose efficient first-order method based on extensions of coordinate descent method to learn the optimal solution of ChIP-PIT, which makes it particularly suitable for the analysis of massive scale ChIP-seq data. Experimental evaluation the ENCODE data illustrate the usefulness of the proposed model.

  2. SISTEMI IPTV NA IP MULTIMEDIJSKEM PODSISTEMU

    OpenAIRE

    2013-01-01

    Diplomsko delo obravnava arhitekture sistemov IPTV na IP multimedijskem podsistemu ter pripadajoče storitve in funkcije IPTV na IP multimedijskem podsistemu. Predstavljene arhitekture prinašajo veliko novih funkcionalnosti, ki so podrobneje predstavljene po posameznih enotah. V nalogi je tudi podrobneje opisana arhitektura IP multimedijskega podsistema z ravninami in protokoli. Naloga se zaključuje s storitvami na takšnih arhitekturah.

  3. Security of IP Telephony in Ecuador: Online Analysis

    Directory of Open Access Journals (Sweden)

    José Estrada

    2016-06-01

    Full Text Available Telephony is a global service and thus telephone networks have been a coveted target for criminals. Now that voice can be transported over IP and that multiple services are integrated in a convergent model through Internet, there are more incentives to attack and more attackers. Moreover, the development of open source telephone applications has encouraged the massive use of IP telephony, but not an increased awareness about embedded security risks. Due to the current and intensive adoption of IP telephony systems in Ecuador, we conducted an exploration based on public information to obtain statistics about telephone systems connected to Internet in Ecuador. Additionally, using a deliberately vulnerable IP telephony system, we collected more data to do a preliminary analysis of threats to such systems. We found that hundreds of telephone systems were publicly available on the Internet and using outdated versions of Asterisk-based applications. We also found thousands of malicious interactions on the IP telephony system we deployed on the Internet.

  4. Universal Space IP Transparent Proxy Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Communications applications are strategically moving toward Internet Protocol-based architectures and technologies. Despite IP's huge potential, (e.g. cost...

  5. Network Resource Provisioning for IP over Multi-Granular Optical Networks

    Institute of Scientific and Technical Information of China (English)

    SUN Jian-wei; POO Gee-Swee

    2007-01-01

    In the internet protocol(IP) over multi-granular optical switch network (IP/MG-OXC), the network node is a typical multilayer switch comprising several layers, the IP packet switching (PXC) layer, wavelength switching (WXC) layer and fiber switching (FXC) layer.This network is capable of both IP layer grooming and wavelength grooming in a hierarchical manner.Resource provisioning in the multi-granular network paradigm is called hierarchical grooming problem.An integer linear programming (ILP) model is proposed to formulate the problem.An iterative heuristic approach is developed for solving the problem in large networks.Case study shows that IP/MG-OXC network is much more extendible and can significantly save the overall network cost as compared with IP over wavelength division multiplexing network.

  6. Analysis of two competing TCP/IP connections

    NARCIS (Netherlands)

    Altman, E.; Jiménez, T.; Núñez Queija, R.

    2001-01-01

    Many mathematical models exist for describing the behavior of TCP/IP under an exogenous loss process that does not depend on the window size. The goal of this paper is to present a mathematical analysis of two asymmetric competing TCP connections where loss probabilities are directly related to thei

  7. Design and implementation of a differentiated service based qos model for real-time interactive traffic on constrained bandwidth ip networks

    CERN Document Server

    Chaudhuri, Sruti Gan

    2012-01-01

    In this thesis work, a QoS model for real-time interactive traffic on a real network with constrained bandwidth and real-time traffic has been proposed. The model supports tight guarantees of QoS to real-time interactive traffic without over provisioning of bandwidth. A dynamic scheduling model which is adaptive to input data rate of traffic has been proposed. In this model, A Differentiated Service (DiffServ) based approach is proposed for QoS provisioning. The packets are classified and distributed among finite number of queues with limited buffer based on different priorities and total available bandwidth. The model proposes a mechanism to derive the weighted service rates and queue length distribution so as to meet the requirement of low packet loss and delay for real time interactive traffic in the QoS engineered network. An adaptive queuing strategy is proposed so that minimum bandwidth in used for real time traffic. This ensures maximizing availability to best effort traffic. The model assumes constrai...

  8. Registration of two confection sunflower germplasm Lines, HA-R10 and HA-R11, Resistant to sunflower rust

    Science.gov (United States)

    Two confection sunflower (Helianthus annuus L.) germplasm lines, HA-R10 (Reg. No.xxx, PI670043) and HA-R11 (Reg. No.xxx, PI670044) were developed by the USDA-ARS Sunflower and Plant Biology Research Unit in collaboration with the North Dakota Agricultural Experiment Station and released December, 20...

  9. Comparing genome-wide chromatin profiles using ChIP-chip or ChIP-seq

    NARCIS (Netherlands)

    Johannes, Frank; Wardenaar, Rene; Colomé Tatché, Maria; Mousson, Florence; de Graaf, Petra; Mokry, Michal; Guryev, Victor; Timmers, H. Th. Marc; Cuppen, Edwin; Jansen, Ritsert C.; Bateman, Alex

    2010-01-01

    Motivation: ChIP-chip and ChIP-seq technologies provide genomewide measurements of various types of chromatin marks at an unprecedented resolution. With ChIP samples collected from different tissue types and/ or individuals, we can now begin to characterize stochastic or systematic changes in epigen

  10. Comparing genome-wide chromatin profiles using ChIP-chip or ChIP-seq

    NARCIS (Netherlands)

    Johannes, F.; Wardenaar, R.; Colome-Tatche, M.; Mousson, F.; de Graaf, P.; Mokry, M.; Guryev, V.; Timmers, H.T.; Cuppen, E.; Jansen, R.

    2010-01-01

    MOTIVATION: ChIP-chip and ChIP-seq technologies provide genome-wide measurements of various types of chromatin marks at an unprecedented resolution. With ChIP samples collected from different tissue types and/or individuals, we can now begin to characterize stochastic or systematic changes in epigen

  11. 75 FR 13235 - IP-Enabled Services

    Science.gov (United States)

    2010-03-19

    ... interconnected Voice over Internet Protocol (VoIP) service the discontinuance obligations that apply to domestic... From the Federal Register Online via the Government Publishing Office FEDERAL COMMUNICATIONS COMMISSION 47 CFR Part 63 IP-Enabled Services AGENCY: Federal Communications Commission ACTION: Final rule...

  12. Performance of VoIP on HSDPA

    DEFF Research Database (Denmark)

    Wang, Bang; Pedersen, Klaus I.; Kolding, Troels E.

    2005-01-01

    scheduler design incorporating VoIP packet aggregation and user multiplexing is proposed and the VoIP capacity is studied for a macro-cellular environment. Results are obtained for different delay budgets and packet scheduling settings, using either blind round robin or a slightly modified version...

  13. Antiplatelet activity of inositol hexaphosphate (IP6).

    Science.gov (United States)

    Vucenik, I; Podczasy, J J; Shamsuddin, A M

    1999-01-01

    Platelet adhesion to endothelial cells, their aggregation and subsequent release of platelet-derived mediators are key steps in the pathogenesis of thrombosis and atherosclerosis. Using impedance technology the effect of inositol hexaphosphate (IP6) on platelet aggregation and adenosine triphosphate (ATP) release were simultaneously measured in whole blood obtained from healthy volunteers (n = 10). The platelets were activated with adenosine diphosphate (ADP) (10 microM), collagen (2 micrograms/mL), or thrombin (1 U/mL) in the presence or absence of IP6. IP6 significantly inhibited platelet aggregation induced with all agonists in a dose-response manner (p IP6 strongly and significantly reduced agonist-induced ATP release (p = 0.00247 for ADP; p = 0.0074 for collagen; p = 0.0069 for thrombin). These data demonstrate that IP6 effectively inhibits human platelet aggregation in vitro, suggesting its potential in reducing the risk for cardiovascular disease.

  14. Using the Social Enterprise Intervention (SEI) and Individual Placement and Support (IPS) models to improve employment and clinical outcomes of homeless youth with mental illness1

    Science.gov (United States)

    Ferguson, Kristin M.

    2013-01-01

    Prior research reveals high unemployment rates among homeless youth. The literature offers many examples of using evidence-informed and evidence-based supported employment models with vulnerable populations to assist them in obtaining and maintaining employment and concurrently addressing mental health challenges. However, there are few examples to date of these models with homeless youth with mental illness. The purpose of this article was thus to describe a methodology for establishing a university-agency research partnership to design, implement, evaluate, and replicate evidence-informed and evidence-based interventions with homeless youth with mental illness to enhance their employment, mental health, and functional outcomes. Data from two studies are used to illustrate the relationship between vocational skill-building/employment and mental health among homeless youth. The article concludes with a discussion of the implications of conducting community-based participatory employment and clinical intervention research. The author highlights the opportunities and tensions associated with this approach. PMID:24294127

  15. Using the Social Enterprise Intervention (SEI) and Individual Placement and Support (IPS) models to improve employment and clinical outcomes of homeless youth with mental illness.

    Science.gov (United States)

    Ferguson, Kristin M

    2013-09-01

    Prior research reveals high unemployment rates among homeless youth. The literature offers many examples of using evidence-informed and evidence-based supported employment models with vulnerable populations to assist them in obtaining and maintaining employment and concurrently addressing mental health challenges. However, there are few examples to date of these models with homeless youth with mental illness. The purpose of this article was thus to describe a methodology for establishing a university-agency research partnership to design, implement, evaluate, and replicate evidence-informed and evidence-based interventions with homeless youth with mental illness to enhance their employment, mental health, and functional outcomes. Data from two studies are used to illustrate the relationship between vocational skill-building/employment and mental health among homeless youth. The article concludes with a discussion of the implications of conducting community-based participatory employment and clinical intervention research. The author highlights the opportunities and tensions associated with this approach.

  16. Myricetin stimulates the absorption of the pro-carcinogen PhIP

    NARCIS (Netherlands)

    Schutte, M.E.; Sandt, J.J.M. van de; Alink, G.M.; Groten, J.P.; Rietjens, I.M.C.M.

    2006-01-01

    The effect of the flavonoid myricetin on the transport of the pro-carcinogen 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) through differentiated Caco-2 monolayers, a model for the intestinal epithelium, is described. Myricetin causes an increase of the transport of PhIP from the apical to

  17. Securing VoIP keeping your VoIP network safe

    CERN Document Server

    (Bud) Bates, Regis J Jr

    2015-01-01

    Securing VoIP: Keeping Your VoIP Network Safe will show you how to take the initiative to prevent hackers from recording and exploiting your company's secrets. Drawing upon years of practical experience and using numerous examples and case studies, technology guru Bud Bates discusses the business realities that necessitate VoIP system security and the threats to VoIP over both wire and wireless networks. He also provides essential guidance on how to conduct system security audits and how to integrate your existing IT security plan with your VoIP system and security plans, helping you prevent

  18. Space Network IP Services (SNIS): An Architecture for Supporting Low Earth Orbiting IP Satellite Missions

    Science.gov (United States)

    Israel, David J.

    2005-01-01

    The NASA Space Network (SN) supports a variety of missions using the Tracking and Data Relay Satellite System (TDRSS), which includes ground stations in White Sands, New Mexico and Guam. A Space Network IP Services (SNIS) architecture is being developed to support future users with requirements for end-to-end Internet Protocol (IP) communications. This architecture will support all IP protocols, including Mobile IP, over TDRSS Single Access, Multiple Access, and Demand Access Radio Frequency (RF) links. This paper will describe this architecture and how it can enable Low Earth Orbiting IP satellite missions.

  19. Fungal Inositol Pyrophosphate IP7 Is Crucial for Metabolic Adaptation to the Host Environment and Pathogenicity

    Science.gov (United States)

    Lev, Sophie; Li, Cecilia; Desmarini, Desmarini; Saiardi, Adolfo; Fewings, Nicole L.; Schibeci, Stephen D.; Sharma, Raghwa; Sorrell, Tania C.

    2015-01-01

    ABSTRACT Inositol pyrophosphates (PP-IPs) comprising inositol, phosphate, and pyrophosphate (PP) are essential for multiple functions in eukaryotes. Their role in fungal pathogens has never been addressed. Cryptococcus neoformans is a model pathogenic fungus causing life-threatening meningoencephalitis. We investigate the cryptococcal kinases responsible for the production of PP-IPs (IP7/IP8) and the hierarchy of PP-IP importance in pathogenicity. Using gene deletion and inositol polyphosphate profiling, we identified Kcs1 as the major IP6 kinase (producing IP7) and Asp1 as an IP7 kinase (producing IP8). We show that Kcs1-derived IP7 is the most crucial PP-IP for cryptococcal drug susceptibility and the production of virulence determinants. In particular, Kcs1 kinase activity is essential for cryptococcal infection of mouse lungs, as reduced fungal burdens were observed in the absence of Kcs1 or when Kcs1 was catalytically inactive. Transcriptome and carbon source utilization analysis suggested that compromised growth of the KCS1 deletion strain (Δkcs1 mutant) in the low-glucose environment of the host lung is due to its inability to utilize alternative carbon sources. Despite this metabolic defect, the Δkcs1 mutant established persistent, low-level asymptomatic pulmonary infection but failed to elicit a strong immune response in vivo and in vitro and was not readily phagocytosed by primary or immortalized monocytes. Reduced recognition of the Δkcs1 cells by monocytes correlated with reduced exposure of mannoproteins on the Δkcs1 mutant cell surface. We conclude that IP7 is essential for fungal metabolic adaptation to the host environment, immune recognition, and pathogenicity. PMID:26037119

  20. Split degenerate states and stable p+ip phases from holography

    Energy Technology Data Exchange (ETDEWEB)

    Nie, Zhang-Yu; Zeng, Hui [Kunming University of Science and Technology, Kunming (China); Key Laboratory of Theoretical Physics, Institute of Theoretical Physics, Chinese Academy of Sciences, Beijing (China); Pan, Qiyuan [Hunan Normal Univ., Key Lab. of Low Dimensional Quantum Structures and Quantum Control of Ministry of Education, and Synergetic Innovation Center for Quantum Effects and Applications, Dept. of Physics, Changsha (China); Zeng, Hua-Bi [Yangzhou University, College of Physics Science and Technology, Yangzhou, Jiangsu (China); National Central University, Department of Physics, Chungli (China)

    2017-02-15

    In this paper, we investigate the p+ip superfluid phases in the complex vector field holographic p-wave model. We find that in the probe limit, the p+ip phase and the p-wave phase are equally stable, hence the p and ip orders can be mixed with an arbitrary ratio to form more general p+λip phases, which are also equally stable with the p-wave and p+ip phases. As a result, the system possesses a degenerate thermal state in the superfluid region. We further study the case on considering the back-reaction on the metric, and we find that the degenerate ground states will be separated into p-wave and p+ip phases, and the p-wave phase is more stable. Finally, due to the different critical temperature of the zeroth order phase transitions from p-wave and p+ip phases to the normal phase, there is a temperature region where the p+ip phase exists but the p-wave phase does not. In this region we find the stable holographic p+ip phase for the first time. (orig.)

  1. Cancer inhibition by inositol hexaphosphate (IP6) and inositol: from laboratory to clinic.

    Science.gov (United States)

    Vucenik, Ivana; Shamsuddin, AbulKalam M

    2003-11-01

    Inositol hexaphosphate (IP6) is a naturally occurring polyphosphorylated carbohydrate that is present in substantial amounts in almost all plant and mammalian cells. It was recently recognized to possess multiple biological functions. A striking anticancer effect of IP6 was demonstrated in different experimental models. Inositol is also a natural constituent possessing moderate anticancer activity. The most consistent and best anticancer results were obtained from the combination of IP6 plus inositol. In addition to reducing cell proliferation, IP6 increases differentiation of malignant cells, often resulting in a reversion to normal phenotype. Exogenously administered IP6 is rapidly taken into the cells and dephosphorylated to lower-phosphate inositol phosphates, which further interfere with signal transduction pathways and cell cycle arrest. Enhanced immunity and antioxidant properties can also contribute to tumor cell destruction. However, the molecular mechanisms underlying this anticancer action are not fully understood. Because it is abundantly present in regular diet, efficiently absorbed from the gastrointestinal tract, and safe, IP6 holds great promise in our strategies for the prevention and treatment of cancer. IP6 plus inositol enhances the anticancer effect of conventional chemotherapy, controls cancer metastases, and improves the quality of life, as shown in a pilot clinical trial. The data strongly argue for the use of IP6 plus inositol in our strategies for cancer prevention and treatment. However, the effectiveness and safety of IP6 plus inositol at therapeutic doses needs to be determined in phase I and phase II clinical trials in humans.

  2. PeakXus: comprehensive transcription factor binding site discovery from ChIP-Nexus and ChIP-Exo experiments.

    Science.gov (United States)

    Hartonen, Tuomo; Sahu, Biswajyoti; Dave, Kashyap; Kivioja, Teemu; Taipale, Jussi

    2016-09-01

    Transcription factor (TF) binding can be studied accurately in vivo with ChIP-exo and ChIP-Nexus experiments. Only fraction of TF binding mechanisms are yet fully understood and accurate knowledge of binding locations and patterns of TFs is key to understanding binding that is not explained by simple positional weight matrix models. ChIP-exo/Nexus experiments can also offer insight on the effect of single nucleotide polymorphism (SNP) at TF binding sites on expression of the target genes. This is an important mechanism of action for disease-causing SNPs at non-coding genomic regions. We describe a peak caller PeakXus that is specifically designed to leverage the increased resolution of ChIP-exo/Nexus and developed with the aim of making as few assumptions of the data as possible to allow discoveries of novel binding patterns. We apply PeakXus to ChIP-Nexus and ChIP-exo experiments performed both in Homo sapiens and in Drosophila melanogaster cell lines. We show that PeakXus consistently finds more peaks overlapping with a TF-specific recognition sequence than published methods. As an application example we demonstrate how PeakXus can be coupled with unique molecular identifiers (UMIs) to measure the effect of a SNP overlapping with a TF binding site on the in vivo binding of the TF. Source code of PeakXus is available at https://github.com/hartonen/PeakXus tuomo.hartonen@helsinki.fi or jussi.taipale@ki.se. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  3. Modeling of drug-mediated CYP3A4 induction by using human iPS cell-derived enterocyte-like cells

    Energy Technology Data Exchange (ETDEWEB)

    Negoro, Ryosuke [Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka 565-0871 (Japan); Takayama, Kazuo [Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka 565-0871 (Japan); The Keihanshin Consortium for Fostering the Next Generation of Global Leaders in Research (K-CONNEX), Kyoto University, Kyoto 606-8302 (Japan); Laboratory of Hepatocyte Regulation, National Institute of Biomedical Innovation, Health and Nutrition, Osaka 567-0085 (Japan); Nagamoto, Yasuhito [Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka 565-0871 (Japan); Laboratory of Hepatocyte Regulation, National Institute of Biomedical Innovation, Health and Nutrition, Osaka 567-0085 (Japan); Sakurai, Fuminori [Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka 565-0871 (Japan); Laboratory of Regulatory Sciences for Oligonucleotide Therapeutics, Clinical Drug Development Project, Graduate School of Pharmaceutical Sciences, Osaka University Osaka 565-0871 (Japan); Tachibana, Masashi [Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka 565-0871 (Japan); Mizuguchi, Hiroyuki, E-mail: mizuguch@phs.osaka-u.ac.jp [Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka 565-0871 (Japan); Laboratory of Hepatocyte Regulation, National Institute of Biomedical Innovation, Health and Nutrition, Osaka 567-0085 (Japan); Global Center for Medical Engineering and Informatics, Osaka University, Osaka 565-0871 (Japan)

    2016-04-15

    Many drugs have potential to induce the expression of drug-metabolizing enzymes, particularly cytochrome P450 3A4 (CYP3A4), in small intestinal enterocytes. Therefore, a model that can accurately evaluate drug-mediated CYP3A4 induction is urgently needed. In this study, we overlaid Matrigel on the human induced pluripotent stem cells-derived enterocyte-like cells (hiPS-ELCs) to generate the mature hiPS-ELCs that could be applied to drug-mediated CYP3A4 induction test. By overlaying Matrigel in the maturation process of enterocyte-like cells, the gene expression levels of intestinal markers (VILLIN, sucrase-isomaltase, intestine-specific homeobox, caudal type homeobox 2, and intestinal fatty acid-binding protein) were enhanced suggesting that the enterocyte-like cells were maturated by Matrigel overlay. The percentage of VILLIN-positive cells in the hiPS-ELCs found to be approximately 55.6%. To examine the CYP3A4 induction potential, the hiPS-ELCs were treated with various drugs. Treatment with dexamethasone, phenobarbital, rifampicin, or 1α,25-dihydroxyvitamin D3 resulted in 5.8-fold, 13.4-fold, 9.8-fold, or 95.0-fold induction of CYP3A4 expression relative to that in the untreated controls, respectively. These results suggest that our hiPS-ELCs would be a useful model for CYP3A4 induction test. - Highlights: • The hiPS-ELCs were matured by Matrigel overlay. • The hiPS-ELCs expressed intestinal nuclear receptors, such as PXR, GR and VDR. • The hiPS-ELC is a useful model for the drug-mediated CYP3A4 induction test.

  4. Comprehensive Comparison of VoIP SIP Protocol Problems and Cisco VoIP System

    Directory of Open Access Journals (Sweden)

    TALAL AL-KHAROBI

    2012-08-01

    Full Text Available Voice over IP (VoIP, use of the packet switched internet for telephony, has improved substantially in the past few years. On the other hand, VoIP has many challenges that do not exist in the public switched telephone network (PSTN, a circuit switched system. VoIP is an application running on the internet, and therefore inherits the internet’s security issues. It is important to realise that VoIP is a relatively young technology, and with any new technology, security typically improves with maturity. This paper provides a comprehensive comparison of a VoIP SIP protocol and CISCO VoIP system. The comparison involves the investigation of the vulnerabilities that target both systems and how secure each system is. With this comparison we present our conclusion on which system is more secure.

  5. A Science and Business Equation for Collaborative Corporate Innovation. Business Strategy, IP Strategy, RandD Strategy: an all-in-one Business Model. A review with a Bio-Technology and Green Chemistry Focus

    OpenAIRE

    Serge Rebouillat

    2013-01-01

    Not all the best people work for you... This is likely one of the motivator for open-innovation in terms of future business development. IP, RandD and Business strategies are becoming one. The adjacent technology analysis, ATA©, goes beyond prior art and freedom to operate traditional approaches providing a new role to the IP strategist still preventing excessive lawyering. Coaching and mediating turn out to be part of this role given the broad interdisciplinary and multi-science skills requi...

  6. Active Network Supports for Mobile IP

    Institute of Scientific and Technical Information of China (English)

    LU Yueming; QIAN Depei; XU Bin; WANG Lei

    2001-01-01

    The basic mobile IP protocol is difficult to implement on the traditional IP network and not flexible and efficient under certain conditions. For example, firewalls or boundary routers may drop packets sent by mobile nodes for security reasons. Traditional networking devices such as routers cannot dynamically load and unload extended services to achieve different qualities of services. In this paper, a new scheme of using the active network to support the mobile IP is presented. The Softnet, a prototype of active networks based on mobile agents,is introduced. The active network is characterized by the programmability of its intermediatenodes and therefore presents dynamic and flexible behaviors. Special services can be dynamically deployed onto the active nodes in the Softnet. This property is definitely required in implementing the mobile IP protocols. The Softnet supports not only the basic mobile IP protocol but also other extended mobile IP protocols. Virtual networks for mobile IP services are dynamically formed by mobile agents in the Softnet to provide different qualities of services.

  7. What are suspicious VoIP delays?

    CERN Document Server

    Mazurczyk, Wojciech; Szczypiorski, Krzysztof

    2010-01-01

    Voice over IP (VoIP) is unquestionably the most popular real-time service in IP networks today. Recent studies have shown that it is also a suitable carrier for information hiding. Hidden communication may pose security concerns as it can lead to confidential information leakage. In VoIP, RTP (Real-time Transport Protocol) in particular, which provides the means for the successful transport of voice packets through IP networks, is suitable for steganographic purposes. It is characterised by a high packet rate compared to other protocols used in IP telephony, resulting in a potentially high steganographic bandwidth. The modification of an RTP packet stream provides many opportunities for hidden communication as the packets may be delayed, reordered or intentionally lost. In this paper, to enable the detection of steganographic exchanges in VoIP, we examined real RTP traffic traces to answer the questions, what do the "normal" delays in RTP packet streams look like? and, is it possible to detect the use of know...

  8. Computational insights into the selectivity mechanism of APP-IP over matrix metalloproteinases

    Science.gov (United States)

    Geng, Lingling; Gao, Jian; Cui, Wei; Tang, Yancheng; Ji, Mingjuan; Chen, Bozhen

    2012-12-01

    In this work, selectivity mechanism of APP-IP inhibitor (β-amyloid precursor protein-derived inhibitory peptide) over matrix metalloproteinases (MMPs including MMP-2, MMP-7, MMP-9 and MMP-14) was investigated by molecular modeling methods. Among MMPs, MMP-2 is the most favorable one for APP-IP interacting based on our calculations. The predicted binding affinities can give a good explanation of the activity difference of inhibitor APP-IP. In Comparison with MMP-2/APP-IP complex, the side chain of Tyr214MMP-7 makes the binding pocket so shallow that the whole side chain of Tyr3APP-IP can not be fully embraced, thus unfavorable for the N-terminal of APP-IP binding to MMP-7. The poor selectivity of APP-IP toward MMP-9 is mainly related with the decrease of interaction between the APP-IP C-terminal and MMP-9 due to the bulky side chains of Pro193 and Gln199, which is in agreement with experiment. The mutations at residues P193A and Q199G of MMP-9 alternate the binding pattern of the C-terminal of APP-IP by forming two new hydrogen bonds and hydrophobic interactions with MMP-9. The mutants favor the binding affinity of MMP-9 largely. For MMP-14/APP-IP, the large steric effect of Phe204MMP-14 and the weak contributions of the polar residues Asn231MMP-14 and Thr190MMP-14 could explain why MMP-14 is non-selective for APP-IP interacting. Here, the molecular modeling methods were successfully employed to explore the selective inhibitor of MMPs, and our work gives valuable information for future rational design of selective peptide inhibitors toward individual MMP.

  9. UVEDBA IP TELEFONIJE V MEDNARODNO PODJETJE

    OpenAIRE

    Goršič, Matej

    2009-01-01

    Diploma opisuje postopen prehod iz klasičnega tipa telefonije na IP (Internet Protocol) telefonijo. To je korak, ki so ga mnoga podjetja v svetu že opravila, veliko jih pa še čaka. Predstavili bomo ozadje klasične in IP telefonije ter osnovne gradnike. Poskušali bomo pojasniti, zakaj je zdaj pravi trenutek za ta prehod ter kaj potrebujemo, morda pa bomo tudi koga opogumili za podoben poseg. Opisan je postopni prehod na IP telefonijo. To pomeni, da v prvem koraku še obdržimo klasično centr...

  10. Voice over IP in Wireless Heterogeneous Networks

    DEFF Research Database (Denmark)

    Fathi, Hanane; Chakraborty, Shyam; Prasad, Ramjee

    The convergence of different types of traffic has preceded the convergence of systems and services in a wireless heterogeneous network. Voice and data traffic are usually treated separate in both 2G and 2.5G wireless networks. With advances in packet switching technology and especially......IP communications are difficult to achieve in a time-varying environment due to channel errors and traffic congestion and across different systems. The provision of VoIP in wireless heterogeneous networks requires a set of time-efficient control mechanisms to support a VoIP session with acceptable quality...

  11. IP Address Management Principles and Practice

    CERN Document Server

    Rooney, Timothy

    2010-01-01

    This book will be the first covering the subject of IP address management (IPAM). The practice of IPAM includes the application of network management disciplines to IP address space and associated network services, namely DHCP (Dynamic Host Configuration Protocol) and DNS (Domain Name System). The consequence of inaccurately configuring DHCP is that end users may not be able to obtain IP addresses to access the network. Without proper DNS configuration, usability of the network will greatly suffer as the name-to-address lookup process may fail. Imagine having to navigate to a website or send a

  12. COMPARISON OF THE EFFECTS OF AGING AND IL-6 ON THE HEPATIC INFLAMMATORY RESPONSE IN TWO MODELS OF SYSTEMIC INJURY: SCALD INJURY VERSUS I.P. LPS ADMINISTRATION

    Science.gov (United States)

    Gomez, Christian R.; Nomellini, Vanessa; Baila, Horea; Oshima, Kiyoko; Kovacs, Elizabeth J.

    2011-01-01

    Regardless of age, a marked elevation in circulating IL-6 levels correlates with increased mortality after injury or an inflammatory challenge. We previously reported that aged IL-6 knockout mice given LPS have improved survival and reduced inflammatory response than LPS-treated aged wild type (WT) mice. Herein, we analyzed the effects of aging and IL-6 on the hepatic inflammatory response in two models of systemic injury: dorsal scald (burn) injury versus intraperitoneal LPS administration. At 24 h after burn injury, circulating alanine aminotransferase and hepatic neutrophil accumulation were comparable regardless of age or IL-6 deficiency. However, at this same time point, these indicators of liver damage, in addition to hepatic levels of KC, a neutrophil chemoattractant, were increased in aged WT mice given LPS relative to young WT mice given LPS. The hepatic injury was drastically reduced in aged IL-6 knockout mice given LPS as compared with LPS-exposed aged WT mice. Our results suggest that the nature of the insult will determine the degree of remote injury in aged animals. In addition, the role of IL-6 as a contributing factor of tissue injury may be insult specific. PMID:18636046

  13. Comparison of the effects of aging and IL-6 on the hepatic inflammatory response in two models of systemic injury: scald injury versus i.p. LPS administration.

    Science.gov (United States)

    Gomez, Christian R; Nomellini, Vanessa; Baila, Horea; Oshima, Kiyoko; Kovacs, Elizabeth J

    2009-02-01

    Regardless of age, a marked elevation in circulating IL-6 levels correlates with increased mortality after injury or an inflammatory challenge. We previously reported that aged IL-6 knockout mice given LPS have improved survival and reduced inflammatory response than LPS-treated aged wild type (WT) mice. Herein, we analyzed the effects of aging and IL-6 on the hepatic inflammatory response in two models of systemic injury: dorsal scald (burn) injury versus intraperitoneal LPS administration. At 24 h after burn injury, circulating alanine aminotransferase and hepatic neutrophil accumulation were comparable regardless of age or IL-6 deficiency. However, at this same time point, these indicators of liver damage, in addition to hepatic levels of KC, a neutrophil chemoattractant, were increased in aged WT mice given LPS relative to young WT mice given LPS. The hepatic injury was drastically reduced in aged IL-6 knockout mice given LPS as compared with LPS-exposed aged WT mice. Our results suggest that the nature of the insult will determine the degree of remote injury in aged animals. In addition, the role of IL-6 as a contributing factor of tissue injury may be insult specific.

  14. A SNP in pri-miR-10a is associated with recurrent spontaneous abortion in a Han-Chinese population.

    Science.gov (United States)

    Li, Ying; Wang, Xue-Qin; Zhang, Lu; Lv, Xiao-Dan; Su, Xing; Tian, Shi; Liu, Chun-Mei; Ma, Xu; Xia, Hong-Fei

    2016-02-16

    MicroRNA-10a (miR-10a) has a wide range of functions in nearly all mammalian tissues and is involved in the occurrence of many diseases. However, it remains unknown whether miR-10a is associated with human recurrent spontaneous abortion (RSA). In this study, we found that rs3809783 A > T in miR-10a coding region was significantly associated with the increase of the risk of human unexplained RSA (URSA) acquisition in a Han-Chinese population. The T allele of rs3809783 hindered the production of mature miR-10a. A to T substitution in miR-10a rs3809783 repressed cell proliferation and migratory capacity. Further investigation discovered that Bcl-2-interacting mediator (Bim) was the functional target of miR-10a and inversely regulated Bim expression. Dual-luciferase assay indicated that A allele in miR-10a rs3809783 could more effectively suppress Bim expression than T allele. In addition, A to T substitution in miR-10a rs3809783 attenuated the sensibility of cells to progesterone and its antagonist mifepristone. Collectively, our data suggest that rs3809783 A > T in pri-miR-10a may be conductive to the genetic predisposition to RSA by disrupting the production of mature miR-10a and reinforcing the expression of Bim.

  15. A Voice Priority Queue (VPQ Fair Scheduler for the VoIP over WLANs

    Directory of Open Access Journals (Sweden)

    Kashif Nisar

    2011-02-01

    Full Text Available Transmission of VoIP over packet switching networks is one of the rapidly emerging real-time Internet Protocol. The real-time application of the Voice over Internet Protocol (VoIP is growing rapidly for it is more flexible than the traditional Public Switched Telephone Networks systems (PSTN. Meanwhile, the VoIP deployment on Wireless Local Area Networks (WLANs, which is based on IEEE 802.11 standards, is increasing. Currently, many schedulers have been introduced such as Weighted Fair Queueing (WFQ, Strict Priority (SP General processor sharing (GPS, Deficit Round Robin (DRR, andContention-Aware Temporally fair Scheduling (CATS. Unfortunately, the current scheduling techniques have some drawbacks on real-time applications and therefore will not be able to handle the VoIP packetsin a proper way. The objective of this research is to propose a new scheduler system model for the VoIP application named Voice Priority Queue (VPQ scheduler. The scheduler system model is to ensureefficiency by producing a higher throughput and fairness for VoIP packets. In this paper, only the First Stage of the VPQ packet scheduler and its algorithm are presented. Simulation topologies for VoIP traffic were implemented and analyzed using the Network Simulator (NS-2. The results show that this method can achieve a better and more accurate VoIP quality throughput and fairness index.

  16. Heterogeneous IP Ecosystem enabling Reuse (HIER)

    Science.gov (United States)

    2017-03-22

    lot • For even a 90% yield, per‐chip  costs  would at least be   $2.2M / (48,000 * 0.90) = $51 – Chiplet  price  would be based on this  cost   plus  some...There are not industry‐wide  pricing  models for IP, e.g. • CHIPS users will need to determine fabrication  costs • But also will need to determine ‘value...incidental  management  costs   plus  some markup to incentivize DoD contractors to  participate – The markup value is key to the model maintaining

  17. Congested Link Inference Algorithms in Dynamic Routing IP Network

    Directory of Open Access Journals (Sweden)

    Yu Chen

    2017-01-01

    Full Text Available The performance descending of current congested link inference algorithms is obviously in dynamic routing IP network, such as the most classical algorithm CLINK. To overcome this problem, based on the assumptions of Markov property and time homogeneity, we build a kind of Variable Structure Discrete Dynamic Bayesian (VSDDB network simplified model of dynamic routing IP network. Under the simplified VSDDB model, based on the Bayesian Maximum A Posteriori (BMAP and Rest Bayesian Network Model (RBNM, we proposed an Improved CLINK (ICLINK algorithm. Considering the concurrent phenomenon of multiple link congestion usually happens, we also proposed algorithm CLILRS (Congested Link Inference algorithm based on Lagrangian Relaxation Subgradient to infer the set of congested links. We validated our results by the experiments of analogy, simulation, and actual Internet.

  18. LACK - a VoIP Steganographic Method

    CERN Document Server

    Mazurczyk, Wojciech

    2008-01-01

    The paper presents a new steganographic method called LACK (Lost Audio PaCKets Steganography) which is intended mainly for VoIP. The method is presented in a broader context of network steganography and of VoIP steganography in particular. The analytical results presented in the paper concern the influence of LACK's hidden data insertion procedure on the method's impact on quality of voice transmission and its resistance to steganalysis.

  19. An Implementation of IP-Phone Gateway

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    To implement voice service over packet-based network (Internet) and TDM-based network (PSTN Public Switched Telephone Network), an IP phone gateway is necessary to transform media stream and convert signaling protocols used over both the two networks. In this article, the architecture of the IP-Phone gateway is described firstly. Then the communications mechanism between functional blocks and multi-thread consideration are presented.

  20. Analyzing ChIP-seq data: preprocessing, normalization, differential identification, and binding pattern characterization.

    Science.gov (United States)

    Taslim, Cenny; Huang, Kun; Huang, Tim; Lin, Shili

    2012-01-01

    Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is a high-throughput antibody-based method to study genome-wide protein-DNA binding interactions. ChIP-seq technology allows scientist to obtain more accurate data providing genome-wide coverage with less starting material and in shorter time compared to older ChIP-chip experiments. Herein we describe a step-by-step guideline in analyzing ChIP-seq data including data preprocessing, nonlinear normalization to enable comparison between different samples and experiments, statistical-based method to identify differential binding sites using mixture modeling and local false discovery rates (fdrs), and binding pattern characterization. In addition, we provide a sample analysis of ChIP-seq data using the steps provided in the guideline.

  1. Data exploration, quality control and statistical analysis of ChIP-exo/nexus experiments.

    Science.gov (United States)

    Welch, Rene; Chung, Dongjun; Grass, Jeffrey; Landick, Robert; Keles, Sündüz

    2017-09-06

    ChIP-exo/nexus experiments rely on innovative modifications of the commonly used ChIP-seq protocol for high resolution mapping of transcription factor binding sites. Although many aspects of the ChIP-exo data analysis are similar to those of ChIP-seq, these high throughput experiments pose a number of unique quality control and analysis challenges. We develop a novel statistical quality control pipeline and accompanying R/Bioconductor package, ChIPexoQual, to enable exploration and analysis of ChIP-exo and related experiments. ChIPexoQual evaluates a number of key issues including strand imbalance, library complexity, and signal enrichment of data. Assessment of these features are facilitated through diagnostic plots and summary statistics computed over regions of the genome with varying levels of coverage. We evaluated our QC pipeline with both large collections of public ChIP-exo/nexus data and multiple, new ChIP-exo datasets from Escherichia coli. ChIPexoQual analysis of these datasets resulted in guidelines for using these QC metrics across a wide range of sequencing depths and provided further insights for modelling ChIP-exo data. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

  2. IPS in Europe: the EQOLISE trial.

    Science.gov (United States)

    Burns, Tom; Catty, Jocelyn

    2008-01-01

    IPS has been demonstrated to increase return to open employment significantly in individuals with mental health problems in the US. Previous experience (e.g. with ACT) has demonstrated the sensitivity of complex community mental health interventions to local social and healthcare cultures. Europe has conditions of generally greater employment security than the US and varying (generally higher) unemployment rates and welfare benefits. Evidence of the effectiveness of IPS in these conditions, and its potential variation across them, would guide local policy and provide possible insights into its mechanism. We conducted an RCT of IPS versus high-quality train-and-place vocational rehabilitation in six European centers with very different labor market and health and social care conditions. A sample of 312 individuals with psychotic illness was randomly allocated (50 per site). Inclusion criteria were a minimum of two years' illness duration, with at least one year of continuous unemployment and six months contact with their current mental health services. Follow-up was 18 months. The primary outcome was any open employment, and secondary outcomes included time to employment, duration of employment and hospital admission. IPS was more effective than the Vocational Services for all vocational outcomes. 85 IPS patients (54.5%) worked for at least one day compared to 43 Vocational Service patients (27.6). They were significantly less likely to be rehospitalized. Local unemployment rates explained a significant amount of the variation in IPS effectiveness and both national economic growth and welfare systems influenced overall employment rates in both services. IPS doubles the access to work of people with psychotic illnesses, without any evidence of increased relapse. Its effectiveness is not independent of external circumstances, particularly local unemployment rates.

  3. AN OPTIMIZED SCHEME FOR FAST HANDOFF IN IP-BASED CDMA WIRELESS COMMUNICATION NETWORKS

    Institute of Scientific and Technical Information of China (English)

    段世平; 徐友云; 宋文涛; 罗汉文

    2002-01-01

    This paper proposed an optimized fast handoff scheme for real-time applications in next generation IPbased CDMA wireless networks. The idea is to utilize optimized IP multicasting handoff (based on PIM-SM),which is triggered by CDMA layer-2 functionality. An IP-based cellular network model with WCDMA FDD air interface and IP-based packet traffic is adopted. No special network entities and signaling for handoff are added in our network model. The simulation results show that low delay and low packet-lost-rate can be obtained.

  4. RelationshipbetweentheexpressionofIP-10and IP-10mRNAinperipheralbloodandHBVDNA levelinpatientswithcirrhosis

    Institute of Scientific and Technical Information of China (English)

    Jian Wang; Ping-Ping Wang; Gui-Ju Xiang; Xiao-Bin Hu

    2010-01-01

    BACKGROUND:Post-hepatitic cirrhosis is regarded as common and severe form of liver damage. Interferonγ-inducible protein 10 (IP-10), a member of the non-ELR (glutamic-leucine-arginine) motif CXC chemokine family, has recently been shown to recruit and activate speciifc subsets of leukocytes to sites of inlfammation or an immune response during the development of hepatic cirrhosis. However, the effects of IP-10 and IP-10 mRNA on inlfammatory inifltration at local sites and in the peripheral blood of patients with post-hepatitic cirrhosis as well as their relationship with viral load are still poorly deifned. This study aimed to detect the relationship between the expression of IP-10 in serum, IP-10 mRNA in peripheral blood mononuclear cells (PBMCs), and the levels of HBV DNA in the serum of patients, and to explore their role in the pathogenesis of cirrhosis. METHODS:Typical patients with cirrhosis after HBV infection were selected, and their serum IP-10 concentrations were evaluated with ELISA, the content of IP-10 mRNA in PBMCs was measured by real-time PCR, and the load of HBV DNA in serum and PBMCs was assessed by semi-quantitative analysis of gel imaging. RESULTS:The levels of IP-10 in serum and IP-10 mRNA in PBMCs of patients with cirrhosis were 299.9±77.2 pg/ml and 0.7500±0.1495, respectively. They were higher than those of controls (P CONCLUSIONS:The levels of IP-10 and IP-10 mRNA in the peripheral blood of patients with cirrhosis increase are closely correlated with the load of HBV DNA in serum, and play a key role in the progression of post-hepatitic cirrhosis.

  5. Voice over IP in Wireless Heterogeneous Networks

    DEFF Research Database (Denmark)

    Fathi, Hanane; Chakraborty, Shyam; Prasad, Ramjee

    The convergence of different types of traffic has preceded the convergence of systems and services in a wireless heterogeneous network. Voice and data traffic are usually treated separate in both 2G and 2.5G wireless networks. With advances in packet switching technology and especially with the d...... and to the discruption caused by the user mobility during the session. Voice over IP in Wireless Hetetrogeneous Networks thus investigates and proposes cross-layer techniques for realizing time-efficient control mechanisms for VoIP: signaling, mobility and security.......The convergence of different types of traffic has preceded the convergence of systems and services in a wireless heterogeneous network. Voice and data traffic are usually treated separate in both 2G and 2.5G wireless networks. With advances in packet switching technology and especially....... The focus of Voice over IP in Wierless Heterogeneous Networks is on mechanisms that affect the VoIP user satisfaction  while not explicitly involved in the media session. This relates to the extra delays introduced by the security and the signaling protocols used to set up an authorized VoIP session...

  6. [IPS an ethical paradigm for biomedical research].

    Science.gov (United States)

    Gámez Escalona, José Antonio

    2013-01-01

    One of the greatest advances in molecular and cell biology was the discovery of the Induced Pluripotent Stem cells (iPS) in mice, by Shinya Yamanka and his team in 2006. The possibility that these cells can be generated also in humans opens up unexpected ways of development for biomedicine. Its main contribution is the creation of a strong protocol that takes into account three major advances in biology such as; nuclear transfer techniques, the discovery of transcription factors associated with pluripotency and the isolation of mouse embryonic stem cells. A protocol that can be easily replicated in other laboratories to have the oportunity to design tests that allow modeling of many incurable diseases, drug testing for human cells or explore the possibilities of autologous transplants of tissues or organs. Yamanaka ethical motivation to find an alternative to embryonic stem cells (ES) and prevent the destruction of embryos produced by In Vitro Fertilization techniques (IVF), has proved to be a research model, in which the intuition of the ethical principles and its application in advanced biotechnology projects, has meant the opening of a whole new way of understanding the biology of embryonic development. It is clear that development, biologically understood (puede ser también ″treated″; tratado), is not a one-way street. The possibilities to deepen into the foundations of molecular biology and genetics, along with the expectations of its clinical applications have earned Yamanka the Nobel Prize in Medicine 2012, along with another great scholar Sir John Gurdon, discoverer of nuclear transfer techniques.

  7. Myocardial KChIP2 Expression in Guinea Pig Resolves an Expanded Electrophysiologic Role.

    Science.gov (United States)

    Nassal, Drew M; Wan, Xiaoping; Liu, Haiyan; Deschênes, Isabelle

    2016-01-01

    Cardiac ion channels and their respective accessory subunits are critical in maintaining proper electrical activity of the heart. Studies have indicated that the K+ channel interacting protein 2 (KChIP2), originally identified as an auxiliary subunit for the channel Kv4, a component of the transient outward K+ channel (Ito), is a Ca2+ binding protein whose regulatory function does not appear restricted to Kv4 modulation. Indeed, the guinea pig myocardium does not express Kv4, yet we show that it still maintains expression of KChIP2, suggesting roles for KChIP2 beyond this canonical auxiliary interaction with Kv4 to modulate Ito. In this study, we capitalize on the guinea pig as a system for investigating how KChIP2 influences the cardiac action potential, independent of effects otherwise attributed to Ito, given the endogenous absence of the current in this species. By performing whole cell patch clamp recordings on isolated adult guinea pig myocytes, we observe that knock down of KChIP2 significantly prolongs the cardiac action potential. This prolongation was not attributed to compromised repolarizing currents, as IKr and IKs were unchanged, but was the result of enhanced L-type Ca2+ current due to an increase in Cav1.2 protein. In addition, cells with reduced KChIP2 also displayed lowered INa from reduced Nav1.5 protein. Historically, rodent models have been used to investigate the role of KChIP2, where dramatic changes to the primary repolarizing current Ito may mask more subtle effects of KChIP2. Evaluation in the guinea pig where Ito is absent, has unveiled additional functions for KChIP2 beyond its canonical regulation of Ito, which defines KChIP2 as a master regulator of cardiac repolarization and depolarization.

  8. Legal Protection on IP Cores for System-on-Chip Designs

    Science.gov (United States)

    Kinoshita, Takahiko

    The current semiconductor industry has shifted from vertical integrated model to horizontal specialization model in term of integrated circuit manufacturing. In this circumstance, IP cores as solutions for System-on-Chip (SoC) have become increasingly important for semiconductor business. This paper examines to what extent IP cores of SoC effectively can be protected by current intellectual property system including integrated circuit layout design law, patent law, design law, copyright law and unfair competition prevention act.

  9. WE-MQS-VoIP Priority: An enhanced LTE Downlink Scheduler for voice services with the integration of VoIP priority mode

    Directory of Open Access Journals (Sweden)

    Duy-Huy Nguyen

    2016-07-01

    Full Text Available The Long Term Evolution (LTE is a high data rates and fully All-IP network. It is developed to support well to multimedia services such as Video, VoIP, Gaming, etc. So that, the real-time services such as VoIP, video, etc. need to be optimized. Nevertheless, the deployment of such live stream services having many challenges. Scheduling and allocating radio resource are very important in LTE network, especially with multimedia services such as VoIP. When voice service transmitted over LTE network, it is affected by many network impairments where there are three main factors including packet loss, delay, and jitter. This study proposes a new scheduler which is based on VoIP priority mode,Wideband (WB E-model, QoS- and Channel-Aware (called WE-MQS-VoIP Priority scheduler for voice services in LTE downlink direction. The proposed scheduling scheme is built based on the WB E-model and Maximum Queue Size (MQS. In addition, we integrate the VoIP priority mode into our scheduling scheme. Since the proposed scheduler considers the VoIP priority mode and user perception, thus, it improves significantly the system performance. The results demonstrate that the proposed scheduler not only meets QoS demands of voice calls but also outperforms Modified Largest Weighted Delay First (M-LWDF in terms of delay, Packet Loss Rate (PLR for all number of user (NU and excepting NU equals 30, respectively. For Fairness Index (FI, cell throughput, and Spectral Efficiency (SE, the difference among the packet schedulers is not significant. The performance evaluation is compared in terms of Delay, PLR, Throughput, FI, and SE.

  10. How to Do a Better IP Commercialization in China?

    Institute of Scientific and Technical Information of China (English)

    WU Ming

    2011-01-01

    1. Status of IP commercialization in China A sound social ecosystem for IP (intellectual property) commercialization will not be established in China overnight because the training of IP professionals and the introducing of IP into management are both a process that takes time.

  11. Simulink based VoIP Analysis

    CERN Document Server

    Singh, Hardeep; Mian, M

    2010-01-01

    Voice communication over internet not be possible without a reliable data network, this was first available when distributed network topologies were used in conjunction with data packets. Early network used single centre node network in which a single workstation (Server) is responsible for the communication. This posed problems as if there was a fault with the centre node, (workstation) nothing would work. This problem was solved by the distributed system in which reliability increases by spreading the load between many nodes. The idea of packet switching & distributed network were combined, this combination were increased reliability, speed & responsible for voice communication over internet, Voice-over-IP (VoIP)These data packets travel through a packet-switched network such as the Internet and arrive at their destination where they are decompressed using a compatible Codec (audio coder/decoder) and converted back to analogue audio. This paper deals with the Simulink architecture for VoIP network.

  12. How to Do a Better IP Commercialization in China?

    Institute of Scientific and Technical Information of China (English)

    WU Ming

    2011-01-01

    @@ 1.Status of IP commercialization in China A sound social ecosystem for IP (intellectual property) commercialization will not be established in China overnight because the training of IP professionals and the introducing of IP into management are both a process that takes time. In recent years, the CAS has conducted a series of training programs concerning IP management and service as well as IP team building.Over the past three years, more than 70 senior staffs from CAS institutes have received certificates of IP Specialists from the CAS headquarters after successfully completing a training program.

  13. Real-time services in IP network architectures

    Science.gov (United States)

    Gilardi, Antonella

    1996-12-01

    The worldwide internet system seems to be the success key for the provision of real time multimedia services to both residential and business users and someone says that in such a way broadband networks will have a reason to exist. This new class of applications that use multiple media (voice, video and data) impose constraints to the global network nowadays consisting of subnets with various data links. The attention will be focused on the interconnection of IP non ATM and ATM networks. IETF and ATM forum are currently involved in the developing specifications suited to adapt the connectionless IP protocol to the connection oriented ATM protocol. First of all the link between the ATM and the IP service model has to be set in order to match the QoS and traffic requirements defined in the relative environment. A further significant topic is represented by the mapping of IP resource reservation model onto the ATM signalling and in the end it is necessary to define how the routing works when there are QoS parameters associated. This paper, considering only unicast applications, will examine the above issues taking as a starting point the situation where an host launches as call set up request with the relevant QoS and traffic descriptor and at some point a router at the edge of the ATM network has to decide how forwarding and request in order to establish an end to end link with the right capabilities. The aim is to compare the proposals emerging from different standard bodies to point out convergency or incompatibility.

  14. Encapsulado de servicios IP sobre redes DVB

    OpenAIRE

    Chamero Hortelano, Adrián

    2006-01-01

    La evolución de los servicios IP hacia las aplicaciones de transporte de contenidos en modo de difusión es un campo de explotación e investigación muy activo. Por otro lado, la convergencia de las diferentes redes de transmisión de datos para soportar dichos servicios provenientes de redes IP es un hecho constatable en muchos ejemplos actualmente de los que las redes DVB, inicialmente concebidas para la dfusión de contenidos audiovisuales, forman parte. Las características ofrecidas ...

  15. Secure IP mobility management for VANET

    CERN Document Server

    Taha, Sanaa

    2013-01-01

    This brief presents the challenges and solutions for VANETs' security and privacy problems occurring in mobility management protocols including Mobile IPv6 (MIPv6), Proxy MIPv6 (PMIPv6), and Network Mobility (NEMO). The authors give an overview of the concept of the vehicular IP-address configurations as the prerequisite step to achieve mobility management for VANETs, and review the current security and privacy schemes applied in the three mobility management protocols. Throughout the brief, the authors propose new schemes and protocols to increase the security of IP addresses within VANETs in

  16. Space-Based Voice over IP Networks

    Science.gov (United States)

    Nguyen, Sam P.; Okino, Clayton; Walsh, William; Clare, Loren

    2007-01-01

    In human space exploration missions (e.g. a return to the Moon and for future missions to Mars), there will be a need to provide voice communications services. In this work we focus on the performance of Voice over IP (VoIP) techniques applied to space networks, where long range latencies, simplex links, and significant bit error rates occur. Link layer and network layer overhead issues are examined. Finally, we provide some discussion on issues related to voice conferencing in the space network environment.

  17. Analysis list: Terf2ip [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Terf2ip Embryonic fibroblast + mm9 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Terf...2ip.1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Terf2ip.5.tsv http://dbarchive.bio...sciencedbc.jp/kyushu-u/mm9/target/Terf2ip.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Terf2ip

  18. Deployment of VoIP Technology: QoS Concerns

    OpenAIRE

    Lazzez, Amor; Slimani, Thabet

    2013-01-01

    Voice over IP (VoIP) is an emerging communication service allowing voice transmission over a private or a public IP network. VoIP allows significant benefits for customers and service providers including cost savings, phone and service portability, mobility, and the integration with other applications. Nevertheless, the deployment of the VoIP technology encounters many challenges such as interoperability issues, security issues, and QoS concerns. Among these disadvantages, QoS issues are cons...

  19. 77 FR 33227 - Assessment Questionnaire-IP Sector Specific Agency Risk Self Assessment Tool (IP-SSARSAT)

    Science.gov (United States)

    2012-06-05

    ... SECURITY Assessment Questionnaire--IP Sector Specific Agency Risk Self Assessment Tool (IP-SSARSAT) AGENCY...--Assessment Questionnaire--IP Sector Specific Agency Risk Self Assessment Tool (IP-SSARSAT). DHS previously...-operators and/or security managers often volunteer to conduct an automated self risk assessment....

  20. 76 FR 81955 - Assessment Questionnaire-IP Sector Specific Agency Risk Self Assessment Tool (IP-SSARSAT)

    Science.gov (United States)

    2011-12-29

    ... SECURITY Assessment Questionnaire--IP Sector Specific Agency Risk Self Assessment Tool (IP-SSARSAT) AGENCY... managers often volunteer to conduct an automated self risk assessment. The requested questionnaire...: Assessment Questionnaire--IP Sector Specific Agency Risk Self Assessment Tool (IP-SSARSAT). OMB Number:...

  1. 基于QoS的IP VPN的研究%A QoS-Based IP VPN Architecture Research

    Institute of Scientific and Technical Information of China (English)

    李振东; 谢立

    2003-01-01

    Constructing Virtual Private Network (VPN) over public network infrastructure (Internet) is becoming the foundation of the next generation network service. At present, VPN mainly focuses on the security property, while it is lack of consideration of QoS. On the basis of analyzing the Differentiated Services (Diffserv) and IPsec, we put forward a QoS-based IP VPN Architecture Model which is a tri-tier model that is composed of ESN, ISN and VPN node. It combines encrypted channel mechanism of IPsec and the edge-and-core-model of Diffserv. It provides end-to-end QoS while assures security.

  2. Presence in the IP Multimedia Subsystem

    Directory of Open Access Journals (Sweden)

    Ling Lin

    2007-01-01

    Full Text Available With an ever increasing penetration of Internet Protocol (IP technologies, the wireless industry is evolving the mobile core network towards all-IP network. The IP Multimedia Subsystem (IMS is a standardised Next Generation Network (NGN architectural framework defined by the 3rd Generation Partnership Project (3GPP to bridge the gap between circuit-switched and packet-switched networks and consolidate both sides into on single all-IP network for all services. In this paper, we provide an insight into the limitation of the presence service, one of the fundamental building blocks of the IMS. Our prototype-based study is unique of its kind and helps identifying the factors which limit the scalability of the current version of the presence service (3GPP TS 23.141 version 7.2.0 Release 7 [1], which will in turn dramatically limit the performance of advanced IMS services. We argue that the client-server paradigm behind the current IMS architecture does not suite the requirements of the IMS system, which defies the very purpose of its introduction. We finally elaborate on possible avenues for addressing this problem.

  3. Tracing the spiral arms in IP Pegasi

    NARCIS (Netherlands)

    Baptista, R.; Morales-Rueda, L.; Harlaftis, E.T.; Marsh, T.R.; Steeghs, D.

    2005-01-01

    We report the analysis of time-resolved spectroscopy of IP Pegasi in outburst with eclipse mapping techniques to investigate the location and geometry of the observed spiral structures. We were able to obtain an improved view of the spiral structures with the aid of light curves extracted in

  4. ChIP-chip versus ChIP-seq: Lessons for experimental design and data analysis

    Directory of Open Access Journals (Sweden)

    Karchenko Peter V

    2011-02-01

    Full Text Available Abstract Background Chromatin immunoprecipitation (ChIP followed by microarray hybridization (ChIP-chip or high-throughput sequencing (ChIP-seq allows genome-wide discovery of protein-DNA interactions such as transcription factor bindings and histone modifications. Previous reports only compared a small number of profiles, and little has been done to compare histone modification profiles generated by the two technologies or to assess the impact of input DNA libraries in ChIP-seq analysis. Here, we performed a systematic analysis of a modENCODE dataset consisting of 31 pairs of ChIP-chip/ChIP-seq profiles of the coactivator CBP, RNA polymerase II (RNA PolII, and six histone modifications across four developmental stages of Drosophila melanogaster. Results Both technologies produce highly reproducible profiles within each platform, ChIP-seq generally produces profiles with a better signal-to-noise ratio, and allows detection of more peaks and narrower peaks. The set of peaks identified by the two technologies can be significantly different, but the extent to which they differ varies depending on the factor and the analysis algorithm. Importantly, we found that there is a significant variation among multiple sequencing profiles of input DNA libraries and that this variation most likely arises from both differences in experimental condition and sequencing depth. We further show that using an inappropriate input DNA profile can impact the average signal profiles around genomic features and peak calling results, highlighting the importance of having high quality input DNA data for normalization in ChIP-seq analysis. Conclusions Our findings highlight the biases present in each of the platforms, show the variability that can arise from both technology and analysis methods, and emphasize the importance of obtaining high quality and deeply sequenced input DNA libraries for ChIP-seq analysis.

  5. KONSTRUKSI SOSIAL GURU IPS TENTANG PEMBELAJARAN IPS DI MTS KECAMATAN KOTA SUMENEP

    Directory of Open Access Journals (Sweden)

    Hamidi Rasyid

    2015-08-01

    Full Text Available Tujuan penelitian ini mendeskripsikan tentang (1 mengetahui konstruksi sosial guru IPS, (2 Implementasi pembelajaran guru di Kelas, (3 Paradigma kepala sekolah tentang pembelajaran IPS.Metode penelitian menggunakan kualitatif, tujuan utama dari penelitian ini adalah untuk menggali substansi mendasar dibalik fakta yang terjadi pada guru IPS. Hasil penelitian menunjukkan bahwa guru IPS mengkonstruksikan IPS menjadi empat konstruksi, (1 IPS adalah ilmu pengetahuan sosial yang peserta didik diharuskan bisa bersikap sosial yang baik sebagai perwujudan dari ilmu pengetahuan sosial, (2 IPS adalah mengkaji kehidupan sehari-hari dan masalah sosial yang ada dalam masyarakat, (3 IPS adalah mengkaji tentang hubungan manusia baik individu dengan individu, individu dengan kelompok maupun kelompok dengan kelompok, (4 IPS adalah mata pelajaran yang di dalamnya ada mata pelajaran sejarah, geografi, sosiologi dan ekonomi. This study describes the purpose of (1 determine the social construction of social studies teachers, (2 Implementation of teacher learning in class, (3 the principal of the learning paradigm of social studies. Using qualitative research methods, the main purpose of this study was to explore the fundamental substance behind the facts that occurred in social studies teacher. The results showed that teachers of social studies constructs into four construction, (1 social studies is a social studies that students are required to be good social as the embodiment of social studies, (2 social studies is reviewing everyday life and social problems that exist in the community, (3 social studies is to examine whether individual human relationships with individuals, groups of individuals and groups with the group, (4 social studiess are subjects in which there is the eye the lessons of history, geography, sociology and economics

  6. A Novel Protocol for IP Traceback to Detect DDoS Attack

    Directory of Open Access Journals (Sweden)

    Yogesh Kumar Meena

    2012-07-01

    Full Text Available Distributed Denial of Service (DDoS Attacks continues to pose major threats to the internet. There are so many protocol designed to trace the attackers address. In order to trace back attack source (i.e., IP addresses, we studied the tradeoff between different existing IP Trace back techniques. We developed a Novel protocol to trace the IP address of DDoS attack. The novel protocol is designed by using response 1, Nonce of secure- neighbor as the parameters. We developed a sample network model. We simulate the network model by applying secure-neighbor protocol in qualnet. Through secure-neighbor we retrieve the basic parameter value (Response 1, Nonce and applies the decryption function on Nonce and value of neighbor-timeout to find the attackers IP address. We studied different internet topologies and aspect of DDoS attacks, used internet power low for simulation of internet.

  7. Shrimp miR-10a Is Co-opted by White Spot Syndrome Virus to Increase Viral Gene Expression and Viral Replication

    Science.gov (United States)

    Huang, Jiun-Yan; Kang, Shih-Ting; Chen, I-Tung; Chang, Li-Kwan; Lin, Shih-Shun; Kou, Guang-Hsiung; Chu, Chia-Ying; Lo, Chu-Fang

    2017-01-01

    Members of the microRNA miR-10 family are highly conserved and play many important roles in diverse biological mechanisms, including immune-related responses and cancer-related processes in certain types of cancer. In this study, we found the most highly upregulated shrimp microRNA from Penaeus vannamei during white spot syndrome virus (WSSV) infection was miR-10a. After confirming the expression level of miR-10a by northern blot and quantitative RT-PCR, an in vivo experiment showed that the viral copy number was decreased in miR-10a-inhibited shrimp. We found that miR-10a targeted the 5′ untranslated region (UTR) of at least three viral genes (vp26, vp28, and wssv102), and plasmids that were controlled by the 5′ UTR of these genes produced enhanced luciferase signals in transfected SF9 cells. These results suggest a previously unreported role for shrimp miR-10a and even a new type of host–virus interaction, whereby a co-opts the key cellular regulator miR-10a to globally enhance the translation of viral proteins. PMID:28932224

  8. Tailored Nanoparticle Codelivery of antimiR-21 and antimiR-10b Augments Glioblastoma Cell Kill by Temozolomide: Toward a "Personalized" Anti-microRNA Therapy.

    Science.gov (United States)

    Ananta, Jeyarama S; Paulmurugan, Ramasamy; Massoud, Tarik F

    2016-09-01

    Glioblastoma remains an aggressive brain malignancy with poor prognosis despite advances in multimodal therapy that include standard use of Temozolomide. MicroRNA-21 (miR-21) and microRNA-10b (miR-10b) are oncomiRs overexpressed in glioblastoma, promoting many aspects of cancer biology. We hypothesized that PLGA nanoparticles carrying antisense miR-21 (antimiR-21) and antisense miR-10b (antimiR-10b) might beneficially knockdown endogenous miR-21 and miR-10b function and reprogram cells prior to Temozolomide treatment. PLGA nanoparticles were effective in intracellular delivery of encapsulated oligonucleotides. Concentrations of delivered antimiR-21 and antimiR-10b were optimized and specifically tailored to copy numbers of intracellular endogenous microRNAs. Coinhibition of miR-21 and miR-10b significantly reduced the number of viable cells (by 24%; p < 0.01) and increased (2.9-fold) cell cycle arrest at G2/M phase upon Temozolomide treatment in U87 MG cells. Cell-tailored nanoparticle-assisted concurrent silencing of miR-21 and miR-10b prior to Temozolomide treatment is an effective molecular therapeutic strategy in cell culture, warranting the need for further studies prior to future in vivo "personalized" medicine applications.

  9. PICS: probabilistic inference for ChIP-seq.

    Science.gov (United States)

    Zhang, Xuekui; Robertson, Gordon; Krzywinski, Martin; Ning, Kaida; Droit, Arnaud; Jones, Steven; Gottardo, Raphael

    2011-03-01

    ChIP-seq combines chromatin immunoprecipitation with massively parallel short-read sequencing. While it can profile genome-wide in vivo transcription factor-DNA association with higher sensitivity, specificity, and spatial resolution than ChIP-chip, it poses new challenges for statistical analysis that derive from the complexity of the biological systems characterized and from variability and biases in its sequence data. We propose a method called PICS (Probabilistic Inference for ChIP-seq) for identifying regions bound by transcription factors from aligned reads. PICS identifies binding event locations by modeling local concentrations of directional reads, and uses DNA fragment length prior information to discriminate closely adjacent binding events via a Bayesian hierarchical t-mixture model. It uses precalculated, whole-genome read mappability profiles and a truncated t-distribution to adjust binding event models for reads that are missing due to local genome repetitiveness. It estimates uncertainties in model parameters that can be used to define confidence regions on binding event locations and to filter estimates. Finally, PICS calculates a per-event enrichment score relative to a control sample, and can use a control sample to estimate a false discovery rate. Using published GABP and FOXA1 data from human cell lines, we show that PICS' predicted binding sites were more consistent with computationally predicted binding motifs than the alternative methods MACS, QuEST, CisGenome, and USeq. We then use a simulation study to confirm that PICS compares favorably to these methods and is robust to model misspecification.

  10. The first reported generation of human induced pluripotent stem cells (iPS cells) and iPS cell-derived cardiomyocytes in the Netherlands.

    Science.gov (United States)

    Freund, C; Davis, R P; Gkatzis, K; Ward-van Oostwaard, D; Mummery, C L

    2010-01-01

    One of the recent breakthroughs in stem cell research has been the reprogramming of human somatic cells to an embryonic stem cell (ESC)-like state (induced pluripotent stem cells, iPS cells). Similar to ESCs, iPS cells can differentiate into derivatives of the three germ layers, for example cardiomyocytes, pancreatic cells or neurons. This technique offers a new approach to investigating disease pathogenesis and to the development of novel therapies. It may now be possible to generate iPS cells from somatic cells of patients who suffer from vascular genetic diseases, such as hereditary haemorrhagic telangiectasia (HHT). The iPS cells will have a similar genotype to that of the patient and can be differentiated in vitro into the cell type(s) that are affected in the patient. Thus they will serve as excellent models for a better understanding of mechanisms underlying the disease. This, together with the ability to test new drugs, could potentially lead to novel therapeutic concepts in the near future. Here we report the first derivation of three human iPS cell lines from two healthy individuals and one HHT patient in the Netherlands. The iPS cells resembled ESCs in morphology and expressed typical ESC markers. In vitro, iPS cells could be differentiated into cells of the three germ layers, including beating cardiomyocytes and vascular cells. With this technique it will be possible to establish human cardiovascular disease models from patient biopsies provided by the principal hospitals in the Netherlands. (Neth Heart J 2010;18:51-4.).

  11. IP-Switch网络通信业务%Communication Services of IP-Switch Network

    Institute of Scientific and Technical Information of China (English)

    马泽旭; 邢兆宏

    2005-01-01

    IP-Switch网是基于IP网络、采用软交换体系架构的、新一代网络通信平台,具有强大的业务提供能力及良好的业务扩展能力,给企业带来很大的收益.介绍IP-Switch网的业务种类、接入方式和实现方法.

  12. Opportunities for IP  in Communications Beyond 3G

    DEFF Research Database (Denmark)

    Nielsen, Thomas Toftegaard; Jacobsen, Rune H.

    2005-01-01

    Future communication will be based on TCP/IP as common network and transport layers to provide global connectivity to users and applications. IP is used to provide ubiquitous access across different access networks and exploits the benefits of a common connectivity layer while reducing the cost...... of operation and maintenance of the network. This paper discusses the opportunities for IP in the evolution towards a future broadband, all-IP mobile communication network. In particular, we argue for three opportunities for the future: Interworking access technologies over IP, IP layer transparency...

  13. Stimulation of inositol 1,4,5-trisphosphate (IP3 receptor subtypes by analogues of IP3.

    Directory of Open Access Journals (Sweden)

    Huma Saleem

    Full Text Available Most animal cells express mixtures of the three subtypes of inositol 1,4,5-trisphosphate receptor (IP(3R encoded by vertebrate genomes. Activation of each subtype by different agonists has not hitherto been examined in cells expressing defined homogenous populations of IP(3R. Here we measure Ca(2+ release evoked by synthetic analogues of IP(3 using a Ca(2+ indicator within the lumen of the endoplasmic reticulum of permeabilized DT40 cells stably expressing single subtypes of mammalian IP(3R. Phosphorylation of (1,4,5IP(3 to (1,3,4,5IP(4 reduced potency by ~100-fold. Relative to (1,4,5IP(3, the potencies of IP(3 analogues modified at the 1-position (malachite green (1,4,5IP(3, 2-position (2-deoxy(1,4,5IP(3 or 3-position (3-deoxy(1,4,5IP(3, (1,3,4,5IP(4 were similar for each IP(3R subtype. The potency of an analogue, (1,4,6IP(3, in which the orientations of the 2- and 3-hydroxyl groups were inverted, was also reduced similarly for all three IP(3R subtypes. Most analogues of IP(3 interact similarly with the three IP(3R subtypes, but the decrease in potency accompanying removal of the 1-phosphate from (1,4,5IP(3 was least for IP(3R3. Addition of a large chromophore (malachite green to the 1-phosphate of (1,4,5IP(3 only modestly reduced potency suggesting that similar analogues could be used to measure (1,4,5IP(3 binding optically. These data provide the first structure-activity analyses of key IP(3 analogues using homogenous populations of each mammalian IP(3R subtype. They demonstrate broadly similar structure-activity relationships for all mammalian IP(3R subtypes and establish the potential utility of (1,4,5IP(3 analogues with chromophores attached to the 1-position.

  14. Thymic derived iPs cells can be differentiated into cardiomyocytes.

    Science.gov (United States)

    Li, Jian; Cao, Yin-yin; Ma, Xiao-jing; Liu, Fang; Li, Shuo-lin; Zhang, Jing; Gao, Yan; Wang, Hui-jun; Yuan, Yuan; Ma, Duan; Huang, Guo-ying

    2015-06-01

    Ventricular septal defect (VSD) is one of the common congenital heart malformations. Several factors lead to the development of VSD, including familial causes, exposure to certain drugs, infectious agents, and maternal metabolic disturbances. We considered that induced pluripotent stem (iPS) cells derived from VSD patients can be used to study the origin and pathogenesis of the VSD. Here, we show generation and cardiomyocyte differentiation potential of iPS cells from thymic epithelial cells of a patient with VSD (TECs-VSD) by overexpressing the four factors: OCT4, SOX2, NANOG, and LIN28 with lentiviral vectors. The self-renewal and pluripotency of the VSD-iPS cells was verified in iPS cells by in vitro expression of pluripotency markers and formation of teratoma in vivo. iPS cell lines from VSD patients differentiated into functional cardiomyocytes can serve as a model system for studying the pathophysiology and identifying etiology of VSD.

  15. A Traffic Forecasting Method with Function to Control Residual Error Distribution for IP Access Networks

    Science.gov (United States)

    Kitahara, Takeshi; Furuya, Hiroki; Nakamura, Hajime

    Since traffic in IP access networks is less aggregated than in backbone networks, its variance could be significant and its distribution may be long-tailed rather than Gaussian in nature. Such characteristics make it difficult to forecast traffic volume in IP access networks for appropriate capacity planning. This paper proposes a traffic forecasting method that includes a function to control residual error distribution in IP access networks. The objective of the proposed method is to grasp the statistical characteristics of peak traffic variations, while conventional methods focus on average rather than peak values. In the proposed method, a neural network model is built recursively while weighting residual errors around the peaks. This enables network operators to control the trade-off between underestimation and overestimation errors according to their planning policy. Evaluation with a total of 136 daily traffic volume data sequences measured in actual IP access networks demonstrates the performance of the proposed method.

  16. Adjusting the IP $\\beta$ Functions in RHIC

    CERN Document Server

    Wittmer, W; Pilat, F; Ptitsyn, V; Van Zeijts, J

    2004-01-01

    The beta functions at the IP can be adjusted without perturbation of other optics functions via several approaches. In this paper we describe a scheme based on a vector knob, which assigns fixed values to the different tuning quadrupoles and scales them by a common multiplier. The values for the knob vector were calculated for a lattice without any errors using MADX. Previous studies for the LHC [1] have shown that this approach can meet the design goals. A specific feature of the RHIC lattice is the nested power supply system. To cope with the resulting problems a detailed response matrix analysis has been carried out and different sets of knobs were calculated and compared. The knobs were tested at RHIC during the 2004 run and preliminary results are discussed. Simultaneously a new approach to measure the beam sizes of both colliding beams at the IP, based on the tunability provided by the knobs, was developed and tested.

  17. Steganography of VoIP streams

    CERN Document Server

    Mazurczyk, Wojciech

    2008-01-01

    In this paper, we circumscribe available steganographic techniques that can be used for creating covert channels for VoIP (Voice over Internet Protocol) streams. Apart from characterizing existing steganographic methods we provide new insights by presenting two new techniques. First one is network steganography solution and exploits free/unused fields of the RTCP (Real-Time Control Protocol) and RTP (Real-Time Transport Protocol) protocols. The second method provides hybrid storage-timing covert channel by utilizing delayed audio packets. The results of the experiment, that was performed, regardless of steganalysis, to estimate a total amount of data that can be covertly transferred in VoIP RTP stream during the typical call, are also included in this article.

  18. Automatic Classification of Attacks on IP Telephony

    Directory of Open Access Journals (Sweden)

    Jakub Safarik

    2013-01-01

    Full Text Available This article proposes an algorithm for automatic analysis of attack data in IP telephony network with a neural network. Data for the analysis is gathered from variable monitoring application running in the network. These monitoring systems are a typical part of nowadays network. Information from them is usually used after attack. It is possible to use an automatic classification of IP telephony attacks for nearly real-time classification and counter attack or mitigation of potential attacks. The classification use proposed neural network, and the article covers design of a neural network and its practical implementation. It contains also methods for neural network learning and data gathering functions from honeypot application.

  19. Fast Echo Canceller in IP Telephony Gateway

    Institute of Scientific and Technical Information of China (English)

    黄永峰; 李星

    2003-01-01

    The length of the echo path in the IP telephony system is very long. Generally, the echo canceller is implemented on the IP telephony gateway which needs to perform concurrently multi-channel echo cancellation and voice compression. Hence, the most key technique to design the echo canceller is to reduce greatly the computational requirement. For this reason a number of innovative features to implement a fast echo canceller are presented. The key components of this canceller include: the separation of adaptive and cancel filters, non-real-time adaptation and real-time cancellation, sharing VAD algorithms with the speech codec, the incorporation of delay indexing with zero coefficients, and windowing the adaptive filter coefficients to reduce the cost of DSP during the cancellation. Finally, the performance of the echo canceller is summarized; the results of evaluation show that the performance gains for echo cancellation are significant.

  20. Content Distribution over IP : Developments and Challenges

    OpenAIRE

    Popescu, Adrian; Kouvatsos, Demetres; Remondo, David; Giordano, Stefano

    2006-01-01

    This chapter focuses on the multimedia distribution over Internet IP under the auspices of the NoE Euro-NGI research project "Routing in Overlay Networks (ROVER)". The multimedia distribution is supported by several components such as services, content distribution chain, protocols and standards whilst Internet is used for content, acquisition, management and delivery as well as an Internet; Protocol Television (IPTV) infrastructure with QoS facilities. As the convergence between fi...

  1. A native IP satellite communications system

    Science.gov (United States)

    Koudelka, O.; Schmidt, M.; Ebert, J.; Schlemmer, H.; Kastner-Puschl, S.; Riedler, W.

    2004-08-01

    ≪ In the framework of ESA's ARTES-5 program the Institute of Applied Systems Technology (Joanneum Research) in cooperation with the Department of Communications and Wave Propagation has developed a novel meshed satellite communications system which is optimised for Internet traffic and applications (L*IP—Local Network Interconnection via Satellite Systems Using the IP Protocol Suite). Both symmetrical and asymmetrical connections are supported. Bandwidth on demand and guaranteed quality of service are key features of the system. A novel multi-frequency TDMA access scheme utilises efficient methods of IP encapsulation. In contrast to other solutions it avoids legacy transport network techniques. While the DVB-RCS standard is based on ATM or MPEG transport cells, the solution of the L*IP system uses variable-length cells which reduces the overhead significantly. A flexible and programmable platform based on Linux machines was chosen to allow the easy implementation and adaptation to different standards. This offers the possibility to apply the system not only to satellite communications, but provides seamless integration with terrestrial fixed broadcast wireless access systems. The platform is also an ideal test-bed for a variety of interactive broadband communications systems. The paper describes the system architecture and the key features of the system.

  2. IP MULTICAST GROUP MANAGEMENT FOR BROADCAST LANS DISTRIBUTION

    Institute of Scientific and Technical Information of China (English)

    阿姆贾德

    2001-01-01

    Problems of the current IGMP mechanism were identified, such as unnecessary periodic probing of hosts and leave latency, which wastes bandwidth and cause more traffic and overhead. Alternative mechanism that preserve the IP multicast model but employ join/leave messages to track local group membership for broadcast LANs (shared medium LANs) was proposed in this paper. We describe the implementation requirements of the new mechanism and compare it to existing one, demonstrating that join/leave approach is uniformly superior for the environment of broadcast LANs.

  3. The CASCAM network control system based on TCP/IP

    Science.gov (United States)

    Ren, S. B.; Yao, Y. Q.; Yao, D. Z.

    2004-05-01

    The paper presents the CASCAM network control system based on the TCP/IP protocol. In order to realize the Client/Server model in network communication between the workstation and personal computer efficiently, two C++ classes, client and server, were developed in the system. The script language Tcl/Tk was used in programming the system control pane in the Client. With loading the dynamic library, the Client sends the request to the Server through the network, then the Server analyzes the request and controls the camera system.

  4. miR-10b*, a master inhibitor of the cell cycle, is down-regulated in human breast tumours.

    Science.gov (United States)

    Biagioni, Francesca; Bossel Ben-Moshe, Noa; Fontemaggi, Giulia; Canu, Valeria; Mori, Federica; Antoniani, Barbara; Di Benedetto, Anna; Santoro, Raffaela; Germoni, Sabrina; De Angelis, Fernanda; Cambria, Anna; Avraham, Roi; Grasso, Giuseppe; Strano, Sabrina; Muti, Paola; Mottolese, Marcella; Yarden, Yosef; Domany, Eytan; Blandino, Giovanni

    2012-11-01

    Deregulated proliferation is a hallmark of cancer cells. Here, we show that microRNA-10b* is a master regulator of breast cancer cell proliferation and is downregulated in tumoural samples versus matched peritumoural counterparts. Two canonical CpG islands (5 kb) upstream from the precursor sequence are hypermethylated in the analysed breast cancer tissues. Ectopic delivery of synthetic microRNA-10b* in breast cancer cell lines or into xenograft mouse breast tumours inhibits cell proliferation and impairs tumour growth in vivo, respectively. We identified and validated in vitro and in vivo three novel target mRNAs of miR-10b* (BUB1, PLK1 and CCNA2), which play a remarkable role in cell cycle regulation and whose high expression in breast cancer patients is associated with reduced disease-free survival, relapse-free survival and metastasis-free survival when compared to patients with low expression. This also suggests that restoration of microRNA-10b* expression might have therapeutic promise.

  5. miR-10b*, a master inhibitor of the cell cycle, is down-regulated in human breast tumours

    Science.gov (United States)

    Biagioni, Francesca; Bossel Ben-Moshe, Noa; Fontemaggi, Giulia; Canu, Valeria; Mori, Federica; Antoniani, Barbara; Di Benedetto, Anna; Santoro, Raffaela; Germoni, Sabrina; De Angelis, Fernanda; Cambria, Anna; Avraham, Roi; Grasso, Giuseppe; Strano, Sabrina; Muti, Paola; Mottolese, Marcella; Yarden, Yosef; Domany, Eytan; Blandino, Giovanni

    2012-01-01

    Deregulated proliferation is a hallmark of cancer cells. Here, we show that microRNA-10b* is a master regulator of breast cancer cell proliferation and is downregulated in tumoural samples versus matched peritumoural counterparts. Two canonical CpG islands (5 kb) upstream from the precursor sequence are hypermethylated in the analysed breast cancer tissues. Ectopic delivery of synthetic microRNA-10b* in breast cancer cell lines or into xenograft mouse breast tumours inhibits cell proliferation and impairs tumour growth in vivo, respectively. We identified and validated in vitro and in vivo three novel target mRNAs of miR-10b* (BUB1, PLK1 and CCNA2), which play a remarkable role in cell cycle regulation and whose high expression in breast cancer patients is associated with reduced disease-free survival, relapse-free survival and metastasis-free survival when compared to patients with low expression. This also suggests that restoration of microRNA-10b* expression might have therapeutic promise. PMID:23125021

  6. ChIP-Enrich: gene set enrichment testing for ChIP-seq data.

    Science.gov (United States)

    Welch, Ryan P; Lee, Chee; Imbriano, Paul M; Patil, Snehal; Weymouth, Terry E; Smith, R Alex; Scott, Laura J; Sartor, Maureen A

    2014-07-01

    Gene set enrichment testing can enhance the biological interpretation of ChIP-seq data. Here, we develop a method, ChIP-Enrich, for this analysis which empirically adjusts for gene locus length (the length of the gene body and its surrounding non-coding sequence). Adjustment for gene locus length is necessary because it is often positively associated with the presence of one or more peaks and because many biologically defined gene sets have an excess of genes with longer or shorter gene locus lengths. Unlike alternative methods, ChIP-Enrich can account for the wide range of gene locus length-to-peak presence relationships (observed in ENCODE ChIP-seq data sets). We show that ChIP-Enrich has a well-calibrated type I error rate using permuted ENCODE ChIP-seq data sets; in contrast, two commonly used gene set enrichment methods, Fisher's exact test and the binomial test implemented in Genomic Regions Enrichment of Annotations Tool (GREAT), can have highly inflated type I error rates and biases in ranking. We identify DNA-binding proteins, including CTCF, JunD and glucocorticoid receptor α (GRα), that show different enrichment patterns for peaks closer to versus further from transcription start sites. We also identify known and potential new biological functions of GRα. ChIP-Enrich is available as a web interface (http://chip-enrich.med.umich.edu) and Bioconductor package. © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

  7. Generation of iPS Cells from Granulosa Cells.

    Science.gov (United States)

    Mao, Jian; Liu, Lin

    2016-01-01

    Various types of somatic cells can be reprogrammed to induced pluripotent stem (iPS) cells. Somatic stem cells may generate iPS cells more efficiently than do differentiated cells. We show that granulosa cells exhibit characteristic of somatic stem cells and can be reprogrammed to iPS cells more efficiently or with few factors. Here, we describe generation of mouse and pig iPS cells from granulosa cells with high efficiency.

  8. The current status of iPS cells in cardiac research and their potential for tissue engineering and regenerative medicine.

    Science.gov (United States)

    Martins, Ana M; Vunjak-Novakovic, Gordana; Reis, Rui L

    2014-04-01

    The recent availability of human cardiomyocytes derived from induced pluripotent stem (iPS) cells opens new opportunities to build in vitro models of cardiac disease, screening for new drugs, and patient-specific cardiac therapy. Notably, the use of iPS cells enables studies in the wide pool of genotypes and phenotypes. We describe progress in reprogramming of induced pluripotent stem (iPS) cells towards the cardiac lineage/differentiation. The focus is on challenges of cardiac disease modeling using iPS cells and their potential to produce safe, effective and affordable therapies/applications with the emphasis of cardiac tissue engineering. We also discuss implications of human iPS cells to biological research and some of the future needs.

  9. Dynamic Mobile IP routers in ad hoc networks

    NARCIS (Netherlands)

    Kock, B.A.; Schmidt, J.R.

    2005-01-01

    This paper describes a concept combining mobile IP and ad hoc routing to create a robust mobile network. In this network all nodes are mobile and globally and locally reachable under the same IP address. Essential for implementing this network are the dynamic mobile IP routers. They act as gateways

  10. A Survey of Voice over IP Security Research

    Science.gov (United States)

    Keromytis, Angelos D.

    We present a survey of Voice over IP security research. Our goal is to provide a roadmap for researchers seeking to understand existing capabilities and, and to identify gaps in addressing the numerous threats and vulnerabilities present in VoIP systems. We also briefly discuss the implications of our findings with respect to actual vulnerabilities reported in a variety VoIP products.

  11. Covert Channels in SIP for VoIP Signalling

    Science.gov (United States)

    Mazurczyk, Wojciech; Szczypiorski, Krzysztof

    In this paper, we evaluate available steganographic techniques for SIP (Session Initiation Protocol) that can be used for creating covert channels during signaling phase of VoIP (Voice over IP) call. Apart from characterizing existing steganographic methods we provide new insights by introducing new techniques. We also estimate amount of data that can be transferred in signalling messages for typical IP telephony call.

  12. A Conjoint Analysis of Voice Over IP Attributes.

    Science.gov (United States)

    Zubey, Michael L.; Wagner, William; Otto, James R.

    2002-01-01

    Managers need to understand the tradeoffs associated with voice over Internet protocol (VoIP) networks as compared to the Public Switched Telephone Network (PSTN). This article measures the preference structures between IP telephony and PSTN services using conjoint analysis. The purpose is to suggest VoIP technology attributes that best meet…

  13. Dynamic Mobile IP routers in ad hoc networks

    NARCIS (Netherlands)

    Kock, B.A.; Schmidt, J.R.

    2005-01-01

    This paper describes a concept combining mobile IP and ad hoc routing to create a robust mobile network. In this network all nodes are mobile and globally and locally reachable under the same IP address. Essential for implementing this network are the dynamic mobile IP routers. They act as gateways

  14. Covert Channels in SIP for VoIP signalling

    CERN Document Server

    Mazurczyk, Wojciech

    2008-01-01

    In this paper, we evaluate available steganographic techniques for SIP (Session Initiation Protocol) that can be used for creating covert channels during signaling phase of VoIP (Voice over IP) call. Apart from characterizing existing steganographic methods we provide new insights by introducing new techniques. We also estimate amount of data that can be transferred in signalling messages for typical IP telephony call.

  15. Iconographic Professional Interests Inventory (3IP): A New Validation Study.

    Science.gov (United States)

    Boerchi, Diego; Magnano, Paola

    2015-11-01

    Interests have been a central focus of counselling psychology (and vocational psychology in particular) for over 100 years. The awareness of professional interests increases self-knowledge and provides occupational information. In career counselling, vocational interests are assessed more frequently than any other vocational construct, though early evaluations (before 13 years old) of professional interests are very rare. The aim of this research is to examine the 3IP construct (Iconographic Professional Interests Inventory; an inventory composed of 65 stylised pictures that represent people in the act of performing a job) in depth, testing more models in addition to the 19 vocational areas proposed in the 3IP manual. Results show that most of the vocational areas can be grouped into 4 second-level areas ("things", "people", "leisure", and "culture"). Moreover, Holland's RIASEC model is tested; an accurate selection of items reveals that this model works well using 24 specific jobs. The research concludes that the inventory has good psychometric qualities which can grow further by mean of the increasing, in a targeted way, of the number of jobs.

  16. Iconographic Professional Interests Inventory (3IP: A New Validation Study

    Directory of Open Access Journals (Sweden)

    Diego Boerchi

    2015-11-01

    Full Text Available Interests have been a central focus of counselling psychology (and vocational psychology in particular for over 100 years. The awareness of professional interests increases self-knowledge and provides occupational information. In career counselling, vocational interests are assessed more frequently than any other vocational construct, though early evaluations (before 13 years old of professional interests are very rare. The aim of this research is to examine the 3IP construct (Iconographic Professional Interests Inventory; an inventory composed of 65 stylised pictures that represent people in the act of performing a job in depth, testing more models in addition to the 19 vocational areas proposed in the 3IP manual. Results show that most of the vocational areas can be grouped into 4 second-level areas (“things”, “people”, “leisure”, and “culture”. Moreover, Holland’s RIASEC model is tested; an accurate selection of items reveals that this model works well using 24 specific jobs. The research concludes that the inventory has good psychometric qualities which can grow further by mean of the increasing, in a targeted way, of the number of jobs.

  17. Pipeline for the Analysis of ChIP-seq Data and New Motif Ranking Procedure

    KAUST Repository

    Ashoor, Haitham

    2011-06-01

    This thesis presents a computational methodology for ab-initio identification of transcription factor binding sites based on ChIP-seq data. This method consists of three main steps, namely ChIP-seq data processing, motif discovery and models selection. A novel method for ranking the models of motifs identified in this process is proposed. This method combines multiple factors in order to rank the provided candidate motifs. It combines the model coverage of the ChIP-seq fragments that contain motifs from which that model is built, the suitable background data made up of shuffled ChIP-seq fragments, and the p-value that resulted from evaluating the model on actual and background data. Two ChIP-seq datasets retrieved from ENCODE project are used to evaluate and demonstrate the ability of the method to predict correct TFBSs with high precision. The first dataset relates to neuron-restrictive silencer factor, NRSF, while the second one corresponds to growth-associated binding protein, GABP. The pipeline system shows high precision prediction for both datasets, as in both cases the top ranked motif closely resembles the known motifs for the respective transcription factors.

  18. Impact of genome assembly status on ChIP-Seq and ChIP-PET data mapping

    Directory of Open Access Journals (Sweden)

    Sachs Laurent

    2009-12-01

    Full Text Available Abstract Background ChIP-Seq and ChIP-PET can potentially be used with any genome for genome wide profiling of protein-DNA interaction sites. Unfortunately, it is probable that most genome assemblies will never reach the quality of the human genome assembly. Therefore, it remains to be determined whether ChIP-Seq and ChIP-PET are practicable with genome sequences other than a few (e.g. human and mouse. Findings Here, we used in silico simulations to assess the impact of completeness or fragmentation of genome assemblies on ChIP-Seq and ChIP-PET data mapping. Conclusions Most currently published genome assemblies are suitable for mapping the short sequence tags produced by ChIP-Seq or ChIP-PET.

  19. Quantifying the impact of inter-site heterogeneity on the distribution of ChIP-seq data.

    Science.gov (United States)

    Cairns, Jonathan; Lynch, Andy G; Tavaré, Simon

    2014-01-01

    Chromatin Immunoprecipitation followed by sequencing (ChIP-seq) is a valuable tool for epigenetic studies. Analysis of the data arising from ChIP-seq experiments often requires implicit or explicit statistical modeling of the read counts. The simple Poisson model is attractive, but does not provide a good fit to observed ChIP-seq data. Researchers therefore often either extend to a more general model (e.g., the Negative Binomial), and/or exclude regions of the genome that do not conform to the model. Since many modeling strategies employed for ChIP-seq data reduce to fitting a mixture of Poisson distributions, we explore the problem of inferring the optimal mixing distribution. We apply the Constrained Newton Method (CNM), which suggests the Negative Binomial - Negative Binomial (NB-NB) mixture model as a candidate for modeling ChIP-seq data. We illustrate fitting the NB-NB model with an accelerated EM algorithm on four data sets from three species. Zero-inflated models have been suggested as an approach to improve model fit for ChIP-seq data. We show that the NB-NB mixture model requires no zero-inflation and suggest that in some cases the need for zero inflation is driven by the model's inability to cope with both artifactual large read counts and the frequently observed very low read counts. We see that the CNM-based approach is a useful diagnostic for the assessment of model fit and inference in ChIP-seq data and beyond. Use of the suggested NB-NB mixture model will be of value not only when calling peaks or otherwise modeling ChIP-seq data, but also when simulating data or constructing blacklists de novo.

  20. [Research and Application of iPS Cells in Blood System].

    Science.gov (United States)

    Zhou, Li-Xia; Ye, Jie-Yu; Lian, Qi-Zhou; Yang, Mo

    2015-04-01

    Induced pluripotent stem cells (iPS cells) were first constructed by Takahshi and et al in 2006. They converted the mouse fibroblasts into ES-like cells via viral transduction with four transcription factors (Oct4, Sox2, Klf4 and c-Myc). Since, the significant progress has been made and many researchers have succeeded in inducing iPS cells from other human somatic cells by some novel approaches, such as combining transcriptional factors and small chemicals. IPS cells have significant prospect in clinical application. IPS cells derived from patient somatic cells can be used as a model in studying the pathogenesis of genetic hematological disease and applied in therapeutic screenings. Recent studies suggested that iPS cells can differentiate into red blood cells and platelets in vitro, which may make up a big blood bank for transfusion in future. In this review, current understanding of both recombinant technology of iPS cells and the research progress in hematology are summarized.

  1. KOMPETENSI GURU IPS DALAM PEMBELAJARAN BERBASIS PEDIDIKAN KARAKTER DI SMP MUHAMMADIYAH KOTA TERNATE

    Directory of Open Access Journals (Sweden)

    Suardi Kader

    2014-03-01

    Full Text Available Penelitian ini merupakan penelitian deskriptif kualitatif. Subjek penelitian terdiri atas: semua guru, kepala sekolah, dan siswa sebagai responden. Objek penelitian ini adalah kompetensi guru dalam menerapkan berbagai metode untuk pendidikan karakter  pada pembelajaran IPS yang terintegrasi. Pengumpulan data dilakukan melalui wawancara, dokumentasi dan observasi. Teknik analisis data yang digunakan adalah teknik analisis kualitatif model induktif. Hasil penelitian adalah sebagai berikut: (1 Kompetensi guru IPS dalam pedidikan karakter di SMP Muhammadiyah 1 dan 2 Kota Ternate belum memuaskan. Hal ini disebabkan oleh guru IPS masih kurang memiliki wawasan dasar keilmuan karakter. Di samping itu juga, guru belum aktif dan kreatif, serta memiliki kemampuan untuk mengembangkan wawasan pendidikan karakter ke dalam mata pelajaran IPS. (2 Metode pembelajaran IPS di SMP Muhammadiyah 1 dan SMP Muhammadiyah 2 Kota Ternate masih cenderung bersifat konvensional, yaitu ceramah. (3 Kendala-kendala yang ditemukan  dalam pembelajaran pendidikan karakter di SMP Muhammadiyah 1 dan SMP Muhammadiyah 2 Kota Ternate yaitu berasal dari guru, sarana, dan prasarana yang belum memadai, lemahnya kesadaran peserta didik, dana dan pembiayaan yang masih kurang, program pembelajaran, pembinaan karakter karena masih kurangnya pemahaman dari pembina atau guru, serta buku-buku penunjang yang digunakan untuk kepentingan proses pembelajaran. Kata kunci: kompetensi guru IPS, pembelajaran, dan pendidikan karakter

  2. Efficient genomic correction methods in human iPS cells using CRISPR-Cas9 system.

    Science.gov (United States)

    Li, Hongmei Lisa; Gee, Peter; Ishida, Kentaro; Hotta, Akitsu

    2016-05-15

    Precise gene correction using the CRISPR-Cas9 system in human iPS cells holds great promise for various applications, such as the study of gene functions, disease modeling, and gene therapy. In this review article, we summarize methods for effective editing of genomic sequences of iPS cells based on our experiences correcting dystrophin gene mutations with the CRISPR-Cas9 system. Designing specific sgRNAs as well as having efficient transfection methods and proper detection assays to assess genomic cleavage activities are critical for successful genome editing in iPS cells. In addition, because iPS cells are fragile by nature when dissociated into single cells, a step-by-step confirmation during the cell recovery process is recommended to obtain an adequate number of genome-edited iPS cell clones. We hope that the techniques described here will be useful for researchers from diverse backgrounds who would like to perform genome editing in iPS cells.

  3. How can we integrate IP strategy with corporate strategy? Combining IP strategy with management strategy making process

    OpenAIRE

    田中, 義敏; Tanaka, Yoshitoshi

    2013-01-01

    Intellectual property systems have been introduced and used in manycountries as a global standard. However, its practical utilisation is still not yetmature because of the lack of IP strategy. Each enterprise has to define aunique IP strategy and be differentiated by other enterprises because the outsideenvironment and internal resource are different. IP people have to havebetter understandings on strategy making process, which is fundamental to themanagement field. In addition, IP strategy s...

  4. Stochastic TCO minimization for Video Transmission over IP Networks

    CERN Document Server

    Goudarzi, Pejman

    2012-01-01

    From the viewpoint of service operators the Total Cost of Ownership (TCO) for developing a communication service comprises from two parts; CAPital EXpenditure (CAPEX) and OPerational EXpenditure (OPEX). These two types of costs are interrelated and affect any service provider's deployment strategy. In many traditional methods, selection of critical elements of a new service is performed in a heuristic manner aimed at reducing only the OPEX part of the TCO which is not necessarily optimal. Furthermore, exact cost modeling for such services is not always possible and contains some uncertainties. In the current work, after cost modeling of each video streaming element by capturing the effect of the model uncertainties, the TCO optimization problem for video streaming over IP networks is formulated as a stochastic optimization problem. The solution of the proposed optimization problem can cope with the cost modeling uncertainties and track the dynamism in the TCO and lead to a time-varying optimal solution. Numer...

  5. n Vitro Immunomodulatory Effect of R10 Fraction of Garlic on Viability and Production of TNF-? in CD8+ T Cells

    Directory of Open Access Journals (Sweden)

    T. Ghazanfari

    2014-01-01

    Full Text Available Introduction & Objective: -cells, especially CD8+ T lymphocytes are the most important cells in anti-tumor response. Previously R10 fraction of garlic extract was reported as an immuno-modulator which induced an effective cellular immunity and Th1 responses. In this study the in vitro immunomodulatory effect of R10 on CD8+ T cells viability and production of TNF-? were evaluated. Materials & Methods: In this experimental study, using monoclonal antibodies attached to magnetic beads with isolating columns by magnetic bead method, CD8+ T cells from spleen cells of Balb/C mice were isolated. R10 fraction based on molecular weight was prepared using Ultra filtration. MTT assay was used to evaluate cell viability. TNF-? level was meas-ured in the supernatant of culture of CD8+ T cells by ELISA. Obtained data was compared and analyzed using Nonparametric Test and Keraskel & Wanny's Test tests.. Results: The findings indicate that all dilutions of R10 fraction increased cell viability of CD8+ T cells in comparison with the negative control group and in the presence of ConA with dilution of 1:50 of R10 fraction significantly increased cell viability of CD8+ T Cells com-pared to ConA alone. Secretion of TNF-? significantly increased by all dilutions of R10 frac-tion. Conclusion: These findings suggest that R10 fraction of garlic can be used as an Immuno-modulator drug candidate for induction of cellular Immunity in tumor therapy. Sci J Hamadan Univ Med Sci 2014; 20 (4:273-279

  6. IP Broadcasting Network Communications Security%浅谈 IP 广电网络的通信安全

    Institute of Scientific and Technical Information of China (English)

    吴永伟

    2015-01-01

    The IP digital broadcasting system is a fully digital audio-broadcasting system based on the IP net-works.Its physical structure completely syncretize with the IP networks,it breaks through the function limit of the traditional broadcasting.The IP digital broadcasting system has the security problems of communication,as it base on the IP networks.This paper discusses the security issues about the IP digital broadcasting system.%IP 数字广电网络是一套基于 IP 网络的纯数字化广播系统,基于 IP 网络的数字化广播、直播、点播,推送功能强大,灵活性强,并突破了传统模拟广播系统的局限性。但 IP 数字广电网络系统也继承了IP 网络安全的问题,主要分析 IP 广电网络所面对的安全问题,并提出解决方法。

  7. Impact of artefact removal on ChIP quality metrics in ChIP-seq and ChIP-exo data.

    Directory of Open Access Journals (Sweden)

    Thomas Samuel Carroll

    2014-04-01

    Full Text Available With the advent of ChIP-seq multiplexing technologies and the subsequent increase in ChIP-seq throughput, the development of working standards for the quality assessment of ChIP-seq studies has received significant attention. The ENCODE consortium’s large scale analysis of transcription factor binding and epigenetic marks as well as concordant work on ChIP-seq by other laboratories has established a new generation of ChIP-seq quality control measures. The use of these metrics alongside common processing steps has however not been evaluated. In this study, we investigate the effects of blacklisting and removal of duplicated reads on established metrics of ChIP-seq quality and show that the interpretation of these metrics is highly dependent on the ChIP-seq preprocessing steps applied. Further to this we perform the first investigation of the use of these metrics for ChIP-exo data and make recommendations for the adaptation of the NSC statistic to allow for the assessment of ChIP-exo efficiency.

  8. Impact of artifact removal on ChIP quality metrics in ChIP-seq and ChIP-exo data.

    Science.gov (United States)

    Carroll, Thomas S; Liang, Ziwei; Salama, Rafik; Stark, Rory; de Santiago, Ines

    2014-01-01

    With the advent of ChIP-seq multiplexing technologies and the subsequent increase in ChIP-seq throughput, the development of working standards for the quality assessment of ChIP-seq studies has received significant attention. The ENCODE consortium's large scale analysis of transcription factor binding and epigenetic marks as well as concordant work on ChIP-seq by other laboratories has established a new generation of ChIP-seq quality control measures. The use of these metrics alongside common processing steps has however not been evaluated. In this study, we investigate the effects of blacklisting and removal of duplicated reads on established metrics of ChIP-seq quality and show that the interpretation of these metrics is highly dependent on the ChIP-seq preprocessing steps applied. Further to this we perform the first investigation of the use of these metrics for ChIP-exo data and make recommendations for the adaptation of the NSC statistic to allow for the assessment of ChIP-exo efficiency.

  9. Analysis list: ATF7IP [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ATF7IP Epidermis + hg19 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/target/ATF7IP....1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/target/ATF7IP.5.tsv http://dbarchive.biosciencedbc....jp/kyushu-u/hg19/target/ATF7IP.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/ATF7IP.Epidermis.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/Epidermis.gml ...

  10. Analysis list: Atf7ip [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Atf7ip Pluripotent stem cell + mm9 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Atf7ip....1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Atf7ip.5.tsv http://dbarchive.biosc...iencedbc.jp/kyushu-u/mm9/target/Atf7ip.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Atf7ip.Plurip...otent_stem_cell.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Pluripotent_stem_cell.gml ...

  11. IP-I0 BASED IMMUNOLOGICAL MONITORING

    DEFF Research Database (Denmark)

    2008-01-01

    The present invention relates to an immunological method and, more particularly, a method for measuring cell-mediated immune reactivity (CMI) in mammals based on the production of IP-10.The invention further discloses an assay and a kit for measuring CMI to an antigen using whole blood or other...... suitable biological samples. The methods of the present invention are useful in therapeutic and diagnostic protocols for human, livestock and veterinary and wild life applications, thus the invention further relates to a method for diagnosing an infection in a mammal....

  12. NEW ARCHITECTURE OF IP QOS MANAGEMENT SYSTEM

    Institute of Scientific and Technical Information of China (English)

    Jiao Li; Cui Yidong; Lin Yu; Wang Wendong; Cheng Shiduan

    2006-01-01

    Based on the analysis of current Quality of Service (QoS) management on IP network, new generation of QoS architecture QoSAF is proposed. QoSAF is divided into three layers: resource control layer, network service layer and business layer. QoS management is accomplished by interactivity of layers. In this architecture, mechanism of feedback control enhances the functions of resource management and system monitoring. The principle of design and logical architecture of system is discussed in detail.

  13. Cell Reprogramming, IPS Limitations, and Overcoming Strategies in Dental Bioengineering

    Directory of Open Access Journals (Sweden)

    Gaskon Ibarretxe

    2012-01-01

    Full Text Available The procurement of induced pluripotent stem cells, or IPS cells, from adult differentiated animal cells has the potential to revolutionize future medicine, where reprogrammed IPS cells may be used to repair disease-affected tissues on demand. The potential of IPS cell technology is tremendous, but it will be essential to improve the methodologies for IPS cell generation and to precisely evaluate each clone and subclone of IPS cells for their safety and efficacy. Additionally, the current state of knowledge on IPS cells advises that research on their regenerative properties is carried out in appropriate tissue and organ systems that permit a safe assessment of the long-term behavior of these reprogrammed cells. In the present paper, we discuss the mechanisms of cell reprogramming, current technical limitations of IPS cells for their use in human tissue engineering, and possibilities to overcome them in the particular case of dental regeneration.

  14. Polymer nanoparticles mediated codelivery of antimiR-10b and antimiR-21 for achieving triple negative breast cancer therapy.

    Science.gov (United States)

    Devulapally, Rammohan; Sekar, Narayana M; Sekar, Thillai V; Foygel, Kira; Massoud, Tarik F; Willmann, Jürgen K; Paulmurugan, Ramasamy

    2015-03-24

    The current study shows the therapeutic outcome achieved in triple negative breast cancer (TNBC) by simultaneously antagonizing miR-21-induced antiapoptosis and miR-10b-induced metastasis, using antisense-miR-21-PS and antisense-miR-10b-PS delivered by polymer nanoparticles (NPs). We synthesized the antisense-miR-21 and antisense-miR-10b loaded PLGA-b-PEG polymer NPs and evaluated their cellular uptake, serum stability, release profile, and the subsequent synchronous blocking of endogenous miR-21 and miR-10b function in TNBC cells in culture, and tumor xenografts in living animals using molecular imaging. Results show that multitarget antagonization of endogenous miRNAs could be an efficient strategy for targeting metastasis and antiapoptosis in the treatment of metastatic cancer. Targeted delivery of antisense-miR-21 and antisense-miR-10b coloaded urokinase plasminogen activator receptor (uPAR) targeted polymer NPs treated mice showed substantial reduction in tumor growth at very low dose of 0.15 mg/kg, compared to the control NPs treated mice and 40% reduction in tumor growth compared to scramble peptide conjugated NPs treated mice, thus demonstrating a potential new therapeutic option for TNBC.

  15. A Review on Hot-IP Finding Methods and Its Application in Early DDoS Target Detection

    Directory of Open Access Journals (Sweden)

    Xuan Dau Hoang

    2016-10-01

    Full Text Available On the high-speed connections of the Internet or computer networks, the IP (Internet Protocol packet traffic passing through the network is extremely high, and that makes it difficult for network monitoring and attack detection applications. This paper reviews methods to find the high-occurrence-frequency elements in the data stream and applies the most efficient methods to find Hot-IPs that are high-frequency IP addresses of IP packets passing through the network. Fast finding of Hot-IPs in the IP packet stream can be effectively used in early detection of DDoS (Distributed Denial of Service attack targets and spreading sources of network worms. Research results show that the Count-Min method gives the best overall performance for Hot-IP detection thanks to its low computational complexity, low space requirement and fast processing speed. We also propose an early detection model of DDoS attack targets based on Hot-IP finding, which can be deployed on the target network routers.

  16. Sexual Dimorphism in a Reciprocal Interaction of Ryanodine and IP3 Receptors in the Induction of Hyperalgesic Priming.

    Science.gov (United States)

    Khomula, Eugen V; Ferrari, Luiz F; Araldi, Dionéia; Levine, Jon D

    2017-02-22

    Hyperalgesic priming, a model of pain chronification in the rat, is mediated by ryanodine receptor-dependent calcium release. Although ryanodine induces priming in both sexes, females are 5 orders of magnitude more sensitive, by an estrogen receptor α (EsRα)-dependent mechanism. An inositol 1,4,5-triphosphate (IP3) receptor inhibitor prevented the induction of priming by ryanodine. For IP3 induced priming, females were also more sensitive. IP3-induced priming was prevented by pretreatment with inhibitors of the sarcoendoplasmic reticulum calcium ATPase and ryanodine receptor. Antisense to EsRα prevented the induction of priming by low-dose IP3 in females. The induction of priming by an EsRα agonist was ryanodine receptor-dependent and prevented by the IP3 antagonist. Thus, an EsRα-dependent bidirectional interaction between endoplasmic reticulum IP3 and ryanodine receptor-mediated calcium signaling is present in the induction of hyperalgesic priming, in females. In cultured male DRG neurons, IP3 (100 μm) potentiated depolarization-induced transients produced by extracellular application of high-potassium solution (20 mm, K20), in nociceptors incubated with β-estradiol. This potentiation of depolarization-induced calcium transients was blocked by the IP3 antagonist, and not observed in the absence of IP3 IP3 potentiation was also blocked by ryanodine receptor antagonist. The application of ryanodine (2 nm), instead of IP3, also potentiated K20-induced calcium transients in the presence of β-estradiol, in an IP3 receptor-dependent manner. Our results point to an EsRα-dependent, reciprocal interaction between IP3 and ryanodine receptors that contributes to sex differences in hyperalgesic priming.SIGNIFICANCE STATEMENT The present study demonstrates a mechanism that plays a role in the marked sexual dimorphism observed in a model of the transition to chronic pain, hyperalgesic priming. This mechanism involves a reciprocal interaction between the endoplasmic

  17. Discovering Attack Path Oriented-IP Traceback

    Institute of Scientific and Technical Information of China (English)

    Fu Jianming(傅建明); Zhu Fuxi; Peng Guojun

    2003-01-01

    The technique of IP traceback may effectively block DOS (Denial Of Service) and meet the requirement of the computer forensic, but its accuracy depends upon that condition that each node in the Internet must support IP packet marking or detected agents. So far, this requirement is not satisfied. On the basis of traditional traceroute,this paper investigates the efficiency of discovering path methods from aspects of the size and order of detecting packets, and the length of paths.It points out that the size of padding in probed packets has a slight effect on discovering latency, and the latency with the method of bulk sending-receiving is much smaller than one with the traditional traceroute. Moreover, the loss rate of packets with the technique of TTL (Time To Live) which increases monotonously is less than that with the technique of TTL which decreases monotonously. Lastly,OS (Operating System) passive fingerprint is used as heuristic to predict the length of the discovered path so as to reduce disturbance in network traffic.

  18. IPS - a vision aided navigation system

    Science.gov (United States)

    Börner, Anko; Baumbach, Dirk; Buder, Maximilian; Choinowski, Andre; Ernst, Ines; Funk, Eugen; Grießbach, Denis; Schischmanow, Adrian; Wohlfeil, Jürgen; Zuev, Sergey

    2017-04-01

    Ego localization is an important prerequisite for several scientific, commercial, and statutory tasks. Only by knowing one's own position, can guidance be provided, inspections be executed, and autonomous vehicles be operated. Localization becomes challenging if satellite-based navigation systems are not available, or data quality is not sufficient. To overcome this problem, a team of the German Aerospace Center (DLR) developed a multi-sensor system based on the human head and its navigation sensors - the eyes and the vestibular system. This system is called integrated positioning system (IPS) and contains a stereo camera and an inertial measurement unit for determining an ego pose in six degrees of freedom in a local coordinate system. IPS is able to operate in real time and can be applied for indoor and outdoor scenarios without any external reference or prior knowledge. In this paper, the system and its key hardware and software components are introduced. The main issues during the development of such complex multi-sensor measurement systems are identified and discussed, and the performance of this technology is demonstrated. The developer team started from scratch and transfers this technology into a commercial product right now. The paper finishes with an outlook.

  19. Relationship between Interplanetary (IP) Parameters and Geomagnetic Indices during IP Shock Events of 2005

    Indian Academy of Sciences (India)

    Jatin Rathod; Girija Rajaram; Radharani Alyana; A. Chandrasekhar Reddy; D. S. Misra; C. G. Patil; M. Y. S. Prasad; A. G. Ananth

    2008-03-01

    In the present study, we investigate the possible relationship of IP parameters of solar wind and interplanetary magnetic field with ground-based geomagnetic indices. To carry out the study, we take all the IP shock events listed by Proton Monitor onboard Solar and Heliospheric Observatory (SOHO) during 2005, and plot the time variations of all the IP parameters and geomagnetic parameters (±5 days), centered at the shock arrival time. Next, we obtain scatter plots of absolute values of solar wind parameters such as Vsw, Nsw and Interplanetary Magnetic Field (IMF) components Bx, By, Bz and total B with the values of geomagnetic parameters such as Dst, Kp indices, dayside Magnetopause (MP) distance and Cosmic-Ray Neutron Monitor count (CRNM). The scatter plots show that before the IP shock, the pattern is random with no clear relationship. Following the shock, a clear pattern emerges with a type of relationship being seen – clear for SHARP shocks and less clear for DIFFUSE shocks. A total of 10 shock events for 2005 have been studied. Typical examples of this behaviour are the shock events of January 21, 2005 and May 15, 2005. Our study suggests a definite correlation between changes in the solar wind and interplanetary magnetic field parameters and ground-based geomagnetic response. We are trying to obtain quantitative relationships between these for shock events of 2005.

  20. BUDAYA BANYUMAS SEBAGAI SUMBER BELAJAR IPS DI KABUPATEN BANYUMAS

    Directory of Open Access Journals (Sweden)

    Amin Hidayat

    2011-10-01

    Full Text Available Banyumas culture can be used as a source of learning for junior high students in the region Banyumas. In addition to contributing significantly to the maintenance and preservation of local culture as an integral part of national culture, learning the IPS will be more meaningful for students. Banyumas have the kinds of art, unique cultural traditions, and historical sites with very high philosophical and educational value.The types of art, tradition and history as a component or element of Banyumas Culture which is prominent and important to be appointed are: History of Banyumas, Tradition of Banyumas, Arts and Heritage of Banyumas . Various alternative learning strategies can be chosen and applied social studies teacher in utilizing Banyumas Culture as a source of learning, one of them is, by applying contextual lear-ning model. It is possible to still refer to the applicable curriculum, namely KTSP.   Key words: culture, banyumas, IPS, SMP  

  1. Evaluation of impact of backbone outages in IP networks

    Science.gov (United States)

    Kogan, Yaakov; Choudhury, Gagan L.; Tarapore, Percy

    2004-09-01

    Nationwide IP networks typically include nodes in major cities and the following elements: customer equipment, access routers, backbone routers, peering routers, access links connecting customer equipment to access routers, access routers to backbone routers, and backbone links interconnecting backbone routers. The part of this network consisting of backbone routers and related interconnecting links is referred to as the "backbone". We develop a new approach for accurately computing the Availability measure of IP networks by directly simulating each type of backbone outage event and its impact on traffic loss. We use this approach to quantify availability improvement as a result of introducing various technological changes in the network such as IGP tuning, high availability router architecture, MPLS-TE and Fast Reroute. A situation, where operational backbone links do not have enough spare capacity to carry additional traffic during the outage time, is referred to as bandwidth loss. We concentrate on one unidirectional backbone link and derive asymptotic approximations for the expected bandwidth loss in the framework of generalized Erlang and Engset models when the total number of resource units and request arrival rates are proportionally large. Simulation results demonstrate good accuracy of the approximations.

  2. iPS cells: a source of cardiac regeneration.

    Science.gov (United States)

    Yoshida, Yoshinori; Yamanaka, Shinya

    2011-02-01

    For the treatment of heart failure, a new strategy to improve cardiac function and inhibit cardiac remodeling needs to be established. Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) are pluripotent cells that can differentiate into cell types from all three germ layers both in vitro and in vivo. The therapeutic effect of ES/iPS cell-derived progeny was reported in animal model. Mouse and human somatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs) by the transduction of four transcription factors, Oct 3/4, Sox2, Klf4, and c-Myc. However, the low induction efficiency hinders the clinical application of iPS technology, and efforts have been made to improve the reprogramming efficiency. There are variations in the characteristics in ES/iPS cell lines, and the further understanding is necessary for the applications of ES/iPS cell technology. Some improvements were also made in the methods to induce cardiomyocytes from ES/iPS cells efficiently. This review article is focused on generation of iPS cells, cardiomyocyte differentiation from ES/iPS cells, and transplantation of derived cardiomyocytes.This article is part of a special issue entitled, "Cardiovascular Stem Cells Revisited".

  3. Dbp5, Gle1-IP6 and Nup159

    Science.gov (United States)

    Folkmann, Andrew W.; Noble, Kristen N.; Cole, Charles N.

    2011-01-01

    Gene expression is a stepwise process involving distinct cellular processes including transcription, mRNA (mRNA) processing, mRNA export, and translation. As mRNAs are being synthesized, proteins associate with the RNA to form messenger ribonucleoprotein particles (mRNPs). Previous studies have demonstrated that the RNA-binding protein composition of these mRNPs is dynamic, changing as the mRNP moves through the different steps of gene expression, and playing a critical role in these events. An important step during this maturation process occurs at the cytoplasmic face of the nuclear pore complex (NPC) where the export protein Gle1 bound to inositol hexakisphosphate (IP6) spatially activates the ATP-hydrolysis and mRNP-remodeling activity of the DEAD-box protein Dbp5. Recent work from our laboratory and others has provided important insights into the function and regulation of Dbp5. These include a more detailed explanation of the mechanism of Dbp5 RNP remodeling, the role of Gle1-IP6 in stimulating Dbp5 ATPase activity, and the identification of a novel paradigm for regulation of Dbp5 by Nup159. Based on in vitro biochemical assays, X-ray crystallography, and corresponding in vivo phenotypes, we propose here an updated model of the Dbp5 cycle during mRNP export through the NPC. This takes into account all available data and provides a platform for future studies. PMID:22064466

  4. Low latency IP mobility management: protocol and analysis

    Directory of Open Access Journals (Sweden)

    Guo Xiaobing

    2011-01-01

    Full Text Available Abstract Mobile IP is one of the dominating protocols that enable a mobile node to remain reachable while moving around in the Internet. However, it suffers from long handoff latency and route inefficiency. In this article, we present a novel distributed mobility management architecture, ADA (Asymmetric Double-Agents, which introduces double mobility agents to serve one end-to-end communication. One mobility agent is located close to the MN and the other close to the CN. ADA can achieve both low handoff latency and low transmission latency, which is crucial for improvement of user perceived QoS. It also provides an easy-to-use mechanism for MNs to manage and control each traffic session with a different policy and provide specific QoS support. We apply ADA to MIPv6 communications and present a detailed protocol design. Subsequently, we propose an analytical framework for systematic and thorough performance evaluation of mobile IP-based mobility management protocols. Equipped with this model, we analyze the handoff latency, single interaction delay and total time cost under the bidirectional tunneling mode and the route optimization mode for MIPv6, HMIPv6, CNLP, and ADA. Through both quantitative analysis and NS2-based simulations, we show that ADA significantly outperforms the existing mobility management protocols.

  5. Chromatin immunoprecipitation (ChIP) of plant transcription factors followed by sequencing (ChIP-SEQ) or hybridization to whole genome arrays (ChIP-CHIP)

    NARCIS (Netherlands)

    Kaufmann, K.; Muiño, J.M.; Østerås, M.; Farinelli, L.; Krajewski, P.; Angenent, G.C.

    2010-01-01

    Chromatin immunoprecipitation (ChIP) is a powerful technique to study interactions between transcription factors (TFs) and DNA in vivo. For genome-wide de novo discovery of TF-binding sites, the DNA that is obtained in ChIP experiments needs to be processed for sequence identification. The sequences

  6. Performance Study of Objective Speech Quality Measurement for Modern Wireless-VoIP Communications

    Directory of Open Access Journals (Sweden)

    Chan Wai-Yip

    2009-01-01

    Full Text Available Wireless-VoIP communications introduce perceptual degradations that are not present with traditional VoIP communications. This paper investigates the effects of such degradations on the performance of three state-of-the-art standard objective quality measurement algorithms—PESQ, P.563, and an "extended" E-model. The comparative study suggests that measurement performance is significantly affected by acoustic background noise type and level as well as speech codec and packet loss concealment strategy. On our data, PESQ attains superior overall performance and P.563 and E-model attain comparable performance figures.

  7. In vitro synthesis and purification of PhIP-deoxyguanosine and PhIP-DNA oligomer covalent complexes

    Energy Technology Data Exchange (ETDEWEB)

    Freeman, J.

    1994-12-01

    2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is a heterocyclic amine compound formed when meats are cooked at high temperatures. PhIP damages DNA by forming covalent complexes with DNA carcinogen. In an effort to understand how the binding of PhIP to DNA may cause cancer, it is important to characterize the structures of PhIP-damaged DNA molecules. Our HPLC data support fluorescence and {sup 32}P Post-labeling studies which indicate the formation of several species of 2{prime}deoxyguanosine-(dG) or oligodeoxynucleotide-PhIP adducts. The reaction of PhIP with dG resulted in a reddish precipitate that was likely the major adduct, N-(deoxyguanosin-8-yl)-PhIP (dG-C8-PhIP) adduct, with a more polar adduct fraction remaining in the supernatant. Reversed-phase HPLC analysis of the adducts in the supernatant revealed the existence of species of much shorter retention times than the dG-C8-PhIP adduct, confirming that these species are more polar than dG-C8-PhIP. At least four adducts were formed in the reaction of PhIP with DNA oligomer. HPLC analysis of the PhIP-DNA oligomer supernatant after butanol extractions revealed four unresolved peaks which spectra had maximum wavelengths between 340 and 360 nm. Though adduct peaks were not completely resolved, there was {approximately}3 minutes interval between the DNA oligomer peak and the adduct peaks. Furthermore, fluorescence emission data of the DNA oligomer-PhIP adduct solution show heterogeneous binding. The more polar PhIP adducts were fraction-collected and their structures will be solved by nuclear magnetic resonance or x-ray crystallography.

  8. Loss of miR-10a Activates Lpo and Collaborates with Activated Wnt Signaling in Inducing Intestinal Neoplasia in Female Mice

    DEFF Research Database (Denmark)

    Stadthagen, Gustavo; Tehler, Disa Elisabet; Høyland-Kroghsbo, Nina Molin;

    2013-01-01

    miRNAs are small regulatory RNAs that, due to their considerable potential to target a wide range of mRNAs, are implicated in essentially all biological process, including cancer. miR-10a is particularly interesting considering its conserved location in the Hox cluster of developmental regulators......-related cancer phenotype we observed. This suggests that miR-10a could be used as a potent diagnostic marker for discovering groups of women that are at high risk of developing colorectal carcinoma, which today is one of the leading causes of cancer-related deaths....

  9. Connexin expression and gap-junctional intercellular communication in ES cells and iPS cells.

    Science.gov (United States)

    Oyamada, Masahito; Takebe, Kumiko; Endo, Aya; Hara, Sachiko; Oyamada, Yumiko

    2013-01-01

    Pluripotent stem cells, i.e., embryonic stem (ES) and induced pluripotent stem (iPS) cells, can indefinitely proliferate without commitment and differentiate into all cell lineages. ES cells are derived from the inner cell mass of the preimplantation blastocyst, whereas iPS cells are generated from somatic cells by overexpression of a few transcription factors. Many studies have demonstrated that mouse and human iPS cells are highly similar but not identical to their respective ES cell counterparts. The potential to generate basically any differentiated cell types from these cells offers the possibility to establish new models of mammalian development and to create new sources of cells for regenerative medicine. ES cells and iPS cells also provide useful models to study connexin expression and gap-junctional intercellular communication (GJIC) during cell differentiation and reprogramming. In 1996, we reported connexin expression and GJIC in mouse ES cells. Because a substantial number of papers on these subjects have been published since our report, this Mini Review summarizes currently available data on connexin expression and GJIC in ES cells and iPS cells during undifferentiated state, differentiation, and reprogramming.

  10. Connexin expression and gap-junctional intercellular communication in ES cells and iPS cells

    Directory of Open Access Journals (Sweden)

    Masahito eOyamada

    2013-07-01

    Full Text Available Pluripotent stem cells, i.e., embryonic stem (ES and induced pluripotent stem (iPS cells, can indefinitely proliferate without commitment and differentiate into all cell lineages. ES cells are derived from the inner cell mass of the preimplantation blastocyst, whereas iPS cells are generated from somatic cells by overexpression of a few transcription factors. Many studies have demonstrated that mouse and human iPS cells are highly similar but not identical to their respective ES cell counterparts. The potential to generate basically any differentiated cell types from these cells offers the possibility to establish new models of mammalian development and to create new sources of cells for regenerative medicine. ES cells and iPS cells also provide useful models to study connexin expression and gap-junctional intercellular communication (GJIC during cell differentiation and reprogramming. In 1996, we reported connexin expression and GJIC in mouse ES cells. Because a substantial number of papers on these subjects have been published since our report, this Mini Review summarizes currently available data on connexin expression and GJIC in ES cells and iPS cells during undifferentiated state, differentiation, and reprogramming.

  11. Predictive IP controller for robust position control of linear servo system.

    Science.gov (United States)

    Lu, Shaowu; Zhou, Fengxing; Ma, Yajie; Tang, Xiaoqi

    2016-07-01

    Position control is a typical application of linear servo system. In this paper, to reduce the system overshoot, an integral plus proportional (IP) controller is used in the position control implementation. To further improve the control performance, a gain-tuning IP controller based on a generalized predictive control (GPC) law is proposed. Firstly, to represent the dynamics of the position loop, a second-order linear model is used and its model parameters are estimated on-line by using a recursive least squares method. Secondly, based on the GPC law, an optimal control sequence is obtained by using receding horizon, then directly supplies the IP controller with the corresponding control parameters in the real operations. Finally, simulation and experimental results are presented to show the efficiency of proposed scheme.

  12. Packet flow analysis in IP networks via abstract interpretation

    CERN Document Server

    Komondoor, Raghavan; Seetharam, Deva P; Balodia, Sudha

    2011-01-01

    Static analysis (aka offline analysis) of a model of an IP network is useful for understanding, debugging, and verifying packet flow properties of the network. There have been static analysis approaches proposed in the literature for networks based on model checking as well as graph reachability. Abstract interpretation is a method that has typically been applied to static analysis of programs. We propose a new, abstract-interpretation based approach for analysis of networks. We formalize our approach, mention its correctness guarantee, and demonstrate its flexibility in addressing multiple network-analysis problems that have been previously solved via tailor-made approaches. Finally, we investigate an application of our analysis to a novel problem -- inferring a high-level policy for the network -- which has been addressed in the past only in the restricted single-router setting.

  13. KAJIAN INDUCED PLURIPOTENT STEMCELL (iPS (HARAPAN DAN TANTANGAN

    Directory of Open Access Journals (Sweden)

    Masagus Zainuri

    2014-05-01

    Full Text Available AbstractInduced Pluripotent Stemcell (iPS are adult cells which the genetic information in the nucleus of those cells being reprogrammed (reprogram by inserting exogenous pluripotential genes. The exogenous gene transduction is using vectors, such as lentivirus, retrovirus, or adenovirus, which suppressed the gene expression of the original cells, so they will express the transduced exogenous gene. Viral vectors are then used to reprogramming and producing iPS clones that are pluripotent. iPS derived from adult cells of patient with certain diseases will be used as a tool to study the mechanisms of those specific diseases and the effects of selected drugs against the diseases. Several previous studies have shown that iPS clones developed from specific genetic disease have its original genotype and retain the character of the response to the drug that similar as the original adult cells. Opportunities for the utilization of autologous iPS cell therapy in the future is wide open as expected iPS transplant will not be rejected when transplanted back to the patient. Behind all its potential, iPS production is still facing some problems to be applicable clinically. The use of viruses as vectors may cause problems due to virus gene sequences may be integrated into the genome of the DNA donor cell, thereby causing mutations of the iPS clones. Several subsequent studies have succeeded in replacing the use of viruses as vectors, but the level of efficiency obtained is still very low. Another problem that arises is that epigenetic changes may occur in iPS cultures. Many advanced research related to iPS may be developed in Indonesia and is necessary to improve the production efficiency of iPS and solve iPS clones epigenetic changes problems in the future.Keywords: iPS, pluripotency, transduction, transfection.AbstrakInduced Pluripotent Stemcell (iPS adalah sel somatic dewasa yang informasi genetika dalam inti selnyadiprogram ulang (reprogram dengan cara

  14. Dietary chemoprevention of PhIP induced carcinogenesis in male Fischer 344 rats with tomato and broccoli.

    Directory of Open Access Journals (Sweden)

    Kirstie Canene-Adams

    Full Text Available The heterocyclic amine, 2-amino-1-methyl-6-phenylimidazo[4,5-B]pyridine (PhIP, found in meats cooked at high temperatures, has been implicated in epidemiological and rodent studies for causing breast, prostate, and colorectal cancers. A previous animal study using a xenograft model has shown that whole tomato and broccoli, when eaten in combination, exhibit a marked effect on tumor reduction compared to when eaten alone. Our aim was to determine if PhIP-induced carcinogenesis can be prevented by dietary consumption of whole tomato + broccoli powders. Male Fischer 344 rats (n = 45 were randomized into the following treatment groups: control (AIN93G diet, PhIP (200 ppm in AIN93G diet for the first 20 weeks of the study, or tomato + broccoli + PhIP (mixed in AIN93G diet at 10% each and fed with PhIP for 20 weeks, and then without PhIP for 32 weeks. Study animals were monitored for 52 weeks and were euthanized as necessary based on a set of criteria for health status and tumor burden. Although there appeared to be some hepatic and intestinal toxicity due to the combination of PhIP and tomato + broccoli, these rodents had improved survival and reduced incidence and/or severity of PhIP-induced neoplastic lesions compared to the PhIP-alone treated group. Rats eating tomato + broccoli exhibited a marked decrease in the number and size of cribiform prostatic intraepitheilial neoplasia/carcinoma in situ (cribiform PIN/CIS lesions and in the incidence of invasive intestinal adenocarcinomas and skin carcinomas. Although the apparent toxic effects of combined PhIP and tomato + broccoli need additional study, the results of this study support the hypothesis that a diet rich in tomato and broccoli can reduce or prevent dietary carcinogen-induced cancers.

  15. Contribution of N- and C-terminal Kv4.2 channel domains to KChIP interaction [corrected].

    Science.gov (United States)

    Callsen, Britta; Isbrandt, Dirk; Sauter, Kathrin; Hartmann, L Sven; Pongs, Olaf; Bähring, Robert

    2005-10-15

    Association of Shal gene-related voltage-gated potassium (Kv4) channels with cytoplasmic Kv channel interacting proteins (KChIPs) influences inactivation gating and surface expression. We investigated both functional and biochemical consequences of mutations in cytoplasmic N and C-terminal Kv4.2 domains to characterize structural determinants for KChIP interaction. We performed a lysine-scanning mutagenesis within the proximal 40 amino acid portion and a structure-based mutagenesis in the tetramerization 1 (T1) domain of Kv4.2. In addition, the cytoplasmic Kv4.2 C-terminus was truncated at various positions. Wild-type and mutant Kv4.2 channels were coexpressed with KChIP2 isoforms in mammalian cell lines. The KChIP2-induced modulation of Kv4.2 currents was studied with whole-cell patch clamp and the binding of KChIP2 isoforms to Kv4.2 channels with coimmunoprecipitation experiments. Our results define one major interaction site for KChIPs, including amino acids in the proximal N-terminus between residues 11 and 23, where binding and functional modulation are essentially equivalent. A further interaction site includes residues in the T1 domain. Notably, C-terminal deletions also had marked effects on KChIP2-dependent gating modulation and KChIP2 binding, revealing a previously unknown involvement of domains within the cytoplasmic Kv4.2 C-terminus in KChIP interaction. Less coincidence of binding and functional modulation indicates a more loose 'anchoring' at T1- and C-terminal interaction sites. Our results refine and extend previously proposed structural models for Kv4.2/KChIP complex formation.

  16. Optimal Filtering of Malicious IP Sources

    CERN Document Server

    Soldo, Fabio; Argyraki, Katerina

    2008-01-01

    How can we protect the network infrastructure from malicious traffic, such as scanning, malicious code propagation, and distributed denial-of-service (DDoS) attacks? One mechanism for blocking malicious traffic is filtering: access control lists (ACLs) can selectively block traffic based on fields of the IP header. Filters (ACLs) are already available in the routers today but are a scarce resource because they are stored in the expensive ternary content addressable memory (TCAM). In this paper, we develop, for the first time, a framework for studying filter selection as a resource allocation problem. Within this framework, we study five practical cases of source address/prefix filtering, which correspond to different attack scenarios and operator's policies. We show that filter selection optimization leads to novel variations of the multidimensional knapsack problem and we design optimal, yet computationally efficient, algorithms to solve them. We also evaluate our approach using data from Dshield.org and dem...

  17. 解密家用VoIP

    Institute of Scientific and Technical Information of China (English)

    陈荆花

    2006-01-01

    对一个计算机从业者,人们经常期望他了解所有VoIP技术——如同期望他能恢复硬盘、杀除病毒、组建家庭网络、让用不同语言写的程序兼容运行等等。而事实上,尽管一位计算机从业者可能读过许多行业杂志,或在实验室研究过Asterisk(开放源代码的软件VoIP PBX系统)和SIP网关之类的东西,但真正要在家庭动手实践时,那些理论不一定能解决问题。

  18. TCP/IP Over SpaceWire

    Science.gov (United States)

    Mills, S.; Parkes, S.

    The SpaceWire standard defines a network designed for handling payload data and control information onboard a spacecraft. Among the goals of SpaceWire are re-usability and reliability. The use of network protocols on top of SpaceWire is expected to enhance the already rich re-usability and reliability characteristics of SpaceWire. The Space Systems Research Group at the University of Dundee have developed software to allow data to be sent over SpaceWire using standard network protocols such as TCP/IP, as part of a program of work looking at network protocols for SpaceWire. This paper describes network protocols and their relationship to SpaceWire, introduces software written to allow network protocols to be layered on to SpaceWire, describes the research behind the development of this software, and gives results of some tests and observations made using the software.

  19. Implementation of a Prototype VoIP System

    Institute of Scientific and Technical Information of China (English)

    刘喜成; 李仲同

    2000-01-01

    VoIP (Voice over IP) is a rapidly growing area with great market potential. To promote it for both commercial and research purposes, a prototype VoIP system based on state-of-the-art Motorola communication techniques has been developed. It is a gateway system integrating a PBX and a VoIP module. All com ponents that H.323 defines to support VoIP are implemented in the VoIP module, though in a simplified manner. As an embedded system, the system features real timeness and task distributiveness. A number of additional techniques are used to improve the performance, including noise suppression, zero copy, and buffer structure optimization. When refined in interoperability, the system will also readily serve as a product.

  20. Improvement of CPU time of Linear Discriminant Function based on MNM criterion by IP

    Directory of Open Access Journals (Sweden)

    Shuichi Shinmura

    2014-05-01

    Full Text Available Revised IP-OLDF (optimal linear discriminant function by integer programming is a linear discriminant function to minimize the number of misclassifications (NM of training samples by integer programming (IP. However, IP requires large computation (CPU time. In this paper, it is proposed how to reduce CPU time by using linear programming (LP. In the first phase, Revised LP-OLDF is applied to all cases, and all cases are categorized into two groups: those that are classified correctly or those that are not classified by support vectors (SVs. In the second phase, Revised IP-OLDF is applied to the misclassified cases by SVs. This method is called Revised IPLP-OLDF.In this research, it is evaluated whether NM of Revised IPLP-OLDF is good estimate of the minimum number of misclassifications (MNM by Revised IP-OLDF. Four kinds of the real data—Iris data, Swiss bank note data, student data, and CPD data—are used as training samples. Four kinds of 20,000 re-sampling cases generated from these data are used as the evaluation samples. There are a total of 149 models of all combinations of independent variables by these data. NMs and CPU times of the 149 models are compared with Revised IPLP-OLDF and Revised IP-OLDF. The following results are obtained: 1 Revised IPLP-OLDF significantly improves CPU time. 2 In the case of training samples, all 149 NMs of Revised IPLP-OLDF are equal to the MNM of Revised IP-OLDF. 3 In the case of evaluation samples, most NMs of Revised IPLP-OLDF are equal to NM of Revised IP-OLDF. 4 Generalization abilities of both discriminant functions are concluded to be high, because the difference between the error rates of training and evaluation samples are almost within 2%.   Therefore, Revised IPLP-OLDF is recommended for the analysis of big data instead of Revised IP-OLDF. Next, Revised IPLP-OLDF is compared with LDF and logistic regression by 100-fold cross validation using 100 re-sampling samples. Means of error rates of

  1. 26 CFR 11.404(a)(6)-1 - Time when contributions to “H.R. 10” plans considered made.

    Science.gov (United States)

    2010-04-01

    ...(b) of the Employee Retirement Income Security Act of 1974 (prior to its amendment by the Tax... OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) TEMPORARY INCOME TAX REGULATIONS UNDER THE EMPLOYEE RETIREMENT INCOME SECURITY ACT OF 1974 § 11.404(a)(6)-1 Time when contributions to “H.R. 10” plans...

  2. ATF2 Ultra-Low IP Betas Proposal

    CERN Document Server

    Bambade, P; Bai, S; Braun, H; Delahaye, J P; Marin, E; Schulte, D; Tomás, R; Zimmermann, F; Gao, J; Wang, D; Zhu, XW; Honda, Y; Kuroda, S; Okugi, T; Tauchi, T; Terunuma, N; Urakawa, J; Seryi, A; White, G; Woodley, M; Angal-Kalinin, D; Jones, J; Scarfe, A

    2010-01-01

    The CLIC Final Focus System has considerably larger chromaticity than those of ILC and its scaled test machine ATF2. We propose to reduce the IP betas of ATF2 to reach a CLIC-like chromaticity. This would also allow to study the FFS tuning difficulty as function of the IP beam spot size. Both the ILC and CLIC projects will largely benefit from the ATF2 experience at these ultra-low IP betas.

  3. Mobility and QoS Support in Mobile IP Networks

    Institute of Scientific and Technical Information of China (English)

    DUAN Lian; WU Wei-ling

    2004-01-01

    This paper presents the state of the art of the Quality of Service (QoS) and mobility support mechanisms for mobile IP networks, which includes the issues and challenges in QoS support, an overview of the Mobile IP protocol, a general description of the QoS and Mobility framework, and the End-To-End QoS architecture in the next-generation all-IP mobile network.

  4. 一种基于邻近距离的分布式入侵防御系统模型%Distributed IPS Model Based on Near Neighbor Distance

    Institute of Scientific and Technical Information of China (English)

    张焕; 曹万华; 冯力; 张剑

    2009-01-01

    分析了现有入侵防御系统的体系结构及存在的主要问题;根据入侵防御系统的特点,提出了一种基于邻近距离的分布式入侵防御系统(Intrusion Prevention System,IPS)模型.模型定义了系统中的消息类型,采用基于消息的协作方式可增强系统部署的灵活性,通过计算节点间的邻近距离优化通信范围,并给出相应的消息转发策略,以减少系统的消息量.实验表明,模型可以显著减少分布式入侵防御系统的网络负载.

  5. Motif-based analysis of large nucleotide data sets using MEME-ChIP.

    Science.gov (United States)

    Ma, Wenxiu; Noble, William S; Bailey, Timothy L

    2014-01-01

    MEME-ChIP is a web-based tool for analyzing motifs in large DNA or RNA data sets. It can analyze peak regions identified by ChIP-seq, cross-linking sites identified by CLIP-seq and related assays, as well as sets of genomic regions selected using other criteria. MEME-ChIP performs de novo motif discovery, motif enrichment analysis, motif location analysis and motif clustering, providing a comprehensive picture of the DNA or RNA motifs that are enriched in the input sequences. MEME-ChIP performs two complementary types of de novo motif discovery: weight matrix-based discovery for high accuracy; and word-based discovery for high sensitivity. Motif enrichment analysis using DNA or RNA motifs from human, mouse, worm, fly and other model organisms provides even greater sensitivity. MEME-ChIP's interactive HTML output groups and aligns significant motifs to ease interpretation. This protocol takes less than 3 h, and it provides motif discovery approaches that are distinct and complementary to other online methods.

  6. Identification and characterization of small molecule modulators of KChIP/Kv4 function.

    Science.gov (United States)

    Bowlby, Mark R; Chanda, Pranab; Edris, Wade; Hinson, Joseph; Jow, Flora; Katz, Alan H; Kennedy, Jeffrey; Krishnamurthy, Girija; Pitts, Keith; Ryan, Kevin; Zhang, Howard; Greenblatt, Lynne

    2005-11-15

    Potassium channels and their associated subunits are important contributors to electrical excitability in many cell types. In this study, a yeast two-hybrid assay was used to identify inhibitors such as a diaryl-urea compound (CL-888) that binds to and modulates the formation of the Kv4/KChIP complex. CL-888 altered the apparent affinity of KChIP1 to Kv4.3-N in a Biacore assay, but did not dissociate the two proteins in size-exclusion chromatography experiments. Kv4.2/KChIP1 current amplitude and kinetics were altered with compound exposure, supporting the hypothesis of a compound-induced conformational change in the protein complex. Fluorescence spectroscopy of a unique tryptophan residue in KChIP1 was consistent with compound binding to the protein. Molecular modeling using the KChIP1 crystal structure indicates that compound binding may occur in a small tryptophan-containing binding pocket located on the hydrophilic side of the protein.

  7. Enhancement of arsenic trioxide-mediated changes in human induced pluripotent stem cells (IPS).

    Science.gov (United States)

    Graham, Barbara; Stevens, Jacqueline; Wells, Phatia; Sims, Jennifer; Rogers, Christian; Leggett, Sophia S; Ekunwe, Stephen; Ndebele, Kenneth

    2014-07-22

    Induced pluripotent stem cells (IPS) are an artificially derived type of pluripotent stem cell, showing many of the same characteristics as natural pluripotent stem cells. IPS are a hopeful therapeutic model; however there is a critical need to determine their response to environmental toxins. Effects of arsenic on cells have been studied extensively; however, its effect on IPS is yet to be elucidated. Arsenic trioxide (ATO) has been shown to inhibit cell proliferation, induce apoptosis and genotoxicity in many cells. Based on ATOs action in other cells, we hypothesize that it will induce alterations in morphology, inhibit cell viability and induce a genotoxic effect on IPS. Cells were treated for 24 hours with ATO (0-9 µg/mL). Cell morphology, viability and DNA damage were documented. Results indicated sufficient changes in morphology of cell colonies mainly in cell ability to maintain grouping and ability to remain adherent. Cell viability decreased in a dose dependent manner. There were significant increases in tail length and moment as well as destruction of intact DNA as concentration increased. Exposure to ATO resulted in a reproducible dose dependent sequence of events marked by changes in morphology, decrease of cell viability, and induction of genotoxicity in IPS.

  8. CtIP is required to initiate replication-dependent interstrand crosslink repair.

    Directory of Open Access Journals (Sweden)

    Michelle L Duquette

    Full Text Available DNA interstrand crosslinks (ICLs are toxic lesions that block the progression of replication and transcription. CtIP is a conserved DNA repair protein that facilitates DNA end resection in the double-strand break (DSB repair pathway. Here we show that CtIP plays a critical role during initiation of ICL processing in replicating human cells that is distinct from its role in DSB repair. CtIP depletion sensitizes human cells to ICL inducing agents and significantly impairs the accumulation of DNA damage response proteins RPA, ATR, FANCD2, γH2AX, and phosphorylated ATM at sites of laser generated ICLs. In contrast, the appearance of γH2AX and phosphorylated ATM at sites of laser generated double strand breaks (DSBs is CtIP-independent. We present a model in which CtIP functions early in ICL repair in a BRCA1- and FANCM-dependent manner prior to generation of DSB repair intermediates.

  9. Inositol hexaphosphate (IP6) as an anti-neoplastic and lipid-lowering agent.

    Science.gov (United States)

    Jariwalla, R J

    1999-01-01

    IP6, a major dietary source of inositol phosphates, is a physiological antioxidant with potential to form complexes with cations linked to cell proliferation and hypercholesterolemia. Accordingly, we have examined the action of IP6 on dietary modulation of neoplasia and hyperlipidemia in a Fischer rat model (1, 2). Two studies were conducted on the effects of naturally-derived IP6, administered as purified phytate, a salt form of phytic acid (inositol hexaphosphoric acid). One study examined the effect on the growth of tumors promoted in syngeneic rats transplanted with a viral oncogene-transformed cell line. Increases in tumor incidence and growth rate of fibrosarcomas seen following administration of a special diet (containing 5% saturated fatty acids and 1.2% magnesium oxide) were completely mitigated by supplementation of the same diet with purified potassium-magnesium phytate (8.9% phytic acid by weight). The other study examined the IP6 effect on serum lipid and mineral levels in animals fed a cholesterol-enriched or standard diet. Elevated levels of serum total cholesterol, triglycerides and zinc/copper ratio associated with administration of the cholesterol-enriched diet were significantly lowered by supplementation of this diet with monopotassium phytate. Addition of monopotassium phytate to the standard diet also reduced serum lipid levels but did not significantly affect the zinc/copper ratio. These studies support a role for IP6 as a potential therapeutic agent in the treatment of cancer and hyperlipidemia.

  10. Improvement on QoS of VoIP

    Institute of Scientific and Technical Information of China (English)

    HUANGYongfeng; LIXing; RUANFang

    2003-01-01

    This paper first introduces some factors affecting QoS of VoIP System, and presents some strategies to improve VoIP speech quality. Studies are concentrated on an adaptive algorithms based on IP telephony gateway, which includes adopting buffer to smooth the jitter, low bit rate redundancy packet sending mechanism to recover the packet loss, a delay and loss measurement mechanism based on RTP (Real-time transmit protocol), and an adaptive scheme algorithm according to the loss ratio and end to end delay. Finally, the results of testing have denoted that these adaptive mechanisms applied in gateway can improve greatly QoS of VoIP.

  11. Strategies Towords Next Generation IP Over Optical Networks

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    A consensus is emerging in industry on utilizing an IP-Centric control plane within optical networks to support dynamic provisioning and restoration of lightpaths. At the same time, there are divergent views of how IP routers interact with optical core networks to achieve end-to-end connectivity. This paper describes the strategies of optical communication's future development towards next generation IP over Optical Networks. The desirable extent of network transparency in advanced all-optical network architecture is studied. Architectural alternatives for interconnecting IP routers over optical networks, and the concerned routing and signaling issues are described.

  12. Implementation of direct routing mobile IP for solving triangular routing

    Science.gov (United States)

    Jang, Jongwook; Jang, SeongHo; Park, Miri; Lee, Dae-bong

    2001-10-01

    Mobility support on the network layer is of special importance, as the network layer holds together the huge Internet with common protocol IP. Although based on possibility different wireless or wired technologies, all nodes of the network should be able to communicate. Therefore, mobile IP(an extension of the classical IP) has been designed which enablemobility in the Internet without changing existing wired systems. However, mobile IP leaves some points unsolved. Especially, if it comes to security, efficiency of the packet flow that is called triangular routing. Especially triangular routing can cause unnecessary overhead for the network. Furthermore latency can increase dramatically. This is particularly unfortunate if two communicating hosts are separated by transatlantic links. In order to this problem, many methods like IPv6 and ROMIP are proposed. But these methods have limitation. In other words, they have not compatibility because of needing modification of original IP scheme. Especially ROMIP is very complex and the overhead of control message and processing are serious. The problem of inconsistency of Binding caches may occur too. We therefore propose and analyze the performance of the DRMIP (Direct Routing Mobile IP) which do not modify IP source needed in the sender, is compatible with IP and Mobile IP.

  13. Design and performance evaluation of direct routing mobile IP

    Science.gov (United States)

    Jang, Jongwook; Jang, SeongHo; Kim, Kilyeun; Lee, Jung-Tae

    2001-07-01

    Mobility support on the network layer is of special importance, as the network layer holds together the huge Internet with common protocol IP. Although based on possibility different wireless or wired technologies, all nodes of the network should be able to communicate. Therefore, mobile IP (an extension of the classical IP) has been designed which enables mobility in the Internet without changing existing wired systems. However, mobile IP leaves some points unsolved. Especially, if it comes to security, efficient of the packet flow that is called triangular routing. Especially triangular routing can cause unnecessary overhead for the network. Furthermore latency can increase dramatically. This is particularly unfortunate if two communicating hosts are separated by transatlantic links. In order to this problem, many methods like IPv6 and ROMIP are proposed. But these methods have limitation. In other words, they have not compatibility because of needing modification or original IP scheme. Especially ROMIP is very complex and the overhead of control message and processing are serious. The problem of inconsistency of Binding caches may occur tool. We therefore propose and analyze the DRMIP (Direct Routing Mobile IP) which do not modify IP source needed in the sender, is compatible with IP and Mobile IP.

  14. Distinct iPS Cells Show Different Cardiac Differentiation Efficiency.

    Science.gov (United States)

    Ohno, Yohei; Yuasa, Shinsuke; Egashira, Toru; Seki, Tomohisa; Hashimoto, Hisayuki; Tohyama, Shugo; Saito, Yuki; Kunitomi, Akira; Shimoji, Kenichiro; Onizuka, Takeshi; Kageyama, Toshimi; Yae, Kojiro; Tanaka, Tomofumi; Kaneda, Ruri; Hattori, Fumiyuki; Murata, Mitsushige; Kimura, Kensuke; Fukuda, Keiichi

    2013-01-01

    Patient-specific induced pluripotent stem (iPS) cells can be generated by introducing transcription factors that are highly expressed in embryonic stem (ES) cells into somatic cells. This opens up new possibilities for cell transplantation-based regenerative medicine by overcoming the ethical issues and immunological problems associated with ES cells. Despite the development of various methods for the generation of iPS cells that have resulted in increased efficiency, safety, and general versatility, it remains unknown which types of iPS cells are suitable for clinical use. Therefore, the aims of the present study were to assess (1) the differentiation potential, time course, and efficiency of different types of iPS cell lines to differentiate into cardiomyocytes in vitro and (2) the properties of the iPS cell-derived cardiomyocytes. We found that high-quality iPS cells exhibited better cardiomyocyte differentiation in terms of the time course and efficiency of differentiation than low-quality iPS cells, which hardly ever differentiated into cardiomyocytes. Because of the different properties of the various iPS cell lines such as cardiac differentiation efficiency and potential safety hazards, newly established iPS cell lines must be characterized prior to their use in cardiac regenerative medicine.

  15. Inositol hexaphosphate (IP6): a novel treatment for pancreatic cancer.

    Science.gov (United States)

    Somasundar, Ponnandai; Riggs, Dale R; Jackson, Barbara J; Cunningham, Cynthia; Vona-Davis, Linda; McFadden, David W

    2005-06-15

    Inositol hexaphosphate (IP6) is a naturally occurring polyphosphorylated carbohydrate found in food sources high in fiber content. IP6 has been reported to have significant inhibitory effects against a variety of primary tumors including breast and colon. The effects of IP6 have not been evaluated in pancreatic cancer. We hypothesized that IP6 would significantly inhibit cell growth and increase the apoptotic rate of pancreatic cancer in vitro. Two pancreatic cancer cell lines (MIAPACA and PANC1) were cultured using standard techniques and treated with IP6 at doses of 0.5, 1.0, and 5.0 mm. Cell viability was measured by MTT at 24 and 72 h. Apoptosis was evaluated by Annexin V-FITC and results calculated using FACS analysis. Statistical analysis was performed by ANOVA. Significant reductions (P IP6 concentrations tested in both cell lines and at both time points. Reductions in cell proliferation ranged from 37.1 to 91.5%. IP6 increased early and late apoptotic activity (P IP6 significantly decreased cellular growth and increased apoptosis. Our findings suggest that IP6 has the potential to become an effective adjunct for pancreatic cancer treatment. Further in vivo and human studies are needed to evaluate safety and clinical utility of this agent in patients with pancreatic cancer.

  16. Inositol hexaphosphate (IP6) inhibits cellular proliferation in melanoma.

    Science.gov (United States)

    Rizvi, Irfan; Riggs, Dale R; Jackson, Barbara J; Ng, Alex; Cunningham, Cynthia; McFadden, David W

    2006-06-01

    Inositol Hexaphosphate (IP6) is a naturally occurring polyphosphorylated carbohydrate found in food sources high in fiber content. We have previously reported IP6 to have significant inhibitory effects against pancreatic cancer in vitro. We hypothesized that the IP6 would significantly inhibit cell growth of cutaneous melanoma in vitro. The melanoma line HTB68 was cultured using standard techniques and treated with IP6 at doses ranging from 0.2 to 1.0 mM/well. Cell viability was measured by MTT at 72 h. VEGF production was measured in the cell supernatants by ELISA. Apoptosis was evaluated by Annexin V-FITC and results calculated using FACS analysis. Statistical analysis was performed by ANOVA. Significant reductions (P IP6. Overall, IP6 exhibited a mean inhibition of cell growth of 52.1 +/- 11.5% (range, 1.6-83.0%) at 72 h of incubation. VEGF production was significantly reduced (P IP6 (7.5 pg/ml) compared to control (40.9 pg/ml). IP6 significantly increased (P = 0.029) late apoptosis from 5.3 to 7.0% gated events. No changes in necrosis or early apoptosis were observed. Adjuvant treatment of melanoma continues to challenge clinicians and patients. Our findings that IP6 significantly decreased cellular growth, VEGF production and increased late apoptosis in melanoma suggest its potential therapeutic value. Further in vivo studies are planned to evaluate safety and clinical utility of this agent.

  17. Diffusion of IP Telephony in Undergraduate Private Colleges

    Directory of Open Access Journals (Sweden)

    Patrick C. Olson, PhD

    2007-02-01

    Full Text Available Over the summer of 2000 Menlo College implemented enterprise wide Internet Protocol (IP Telephony (Voice over Internet Protocol [VoIP]. More than five years have passed, and analysts are predicting that in the near future the only available Private Branch Exchange (PBX solutions will be VoIP [4]. In view of this market trend, the diffusion of this technology in these institutions seems slow. The Menlo College implementation is very successful, but has the concept diffused to other institutions? What factors influence the diffusion of this technology to other institutions? This paper examines the status of VoIP at these institutions.

  18. Distinct iPS Cells Show Different Cardiac Differentiation Efficiency

    Directory of Open Access Journals (Sweden)

    Yohei Ohno

    2013-01-01

    Full Text Available Patient-specific induced pluripotent stem (iPS cells can be generated by introducing transcription factors that are highly expressed in embryonic stem (ES cells into somatic cells. This opens up new possibilities for cell transplantation-based regenerative medicine by overcoming the ethical issues and immunological problems associated with ES cells. Despite the development of various methods for the generation of iPS cells that have resulted in increased efficiency, safety, and general versatility, it remains unknown which types of iPS cells are suitable for clinical use. Therefore, the aims of the present study were to assess (1 the differentiation potential, time course, and efficiency of different types of iPS cell lines to differentiate into cardiomyocytes in vitro and (2 the properties of the iPS cell-derived cardiomyocytes. We found that high-quality iPS cells exhibited better cardiomyocyte differentiation in terms of the time course and efficiency of differentiation than low-quality iPS cells, which hardly ever differentiated into cardiomyocytes. Because of the different properties of the various iPS cell lines such as cardiac differentiation efficiency and potential safety hazards, newly established iPS cell lines must be characterized prior to their use in cardiac regenerative medicine.

  19. Maintaining high-quality IP audio services in lossy IP network environments

    Science.gov (United States)

    Barton, Robert J., III; Chodura, Hartmut

    2000-07-01

    In this paper we present our research activities in the area of digital audio processing and transmission. Today's available teleconference audio solutions are lacking in flexibility, robustness and fidelity. There was a need for enhancing the quality of audio for IP-based applications to guarantee optimal services under varying conditions. Multiple tests and user evaluations have shown that a reliable audio communication toolkit is essential for any teleconference application. This paper summarizes our research activities and gives an overview of developed applications. In a first step the parameters, which influence the audio quality, were evaluated. All of these parameters have to be optimized in order to result into the best achievable quality. Therefore it was necessary to enhance existing schemes or develop new methods. Applications were developed for Internet-Telephony, broadcast of live music and spatial audio for Virtual Reality environments. This paper describes these applications and issues of delivering high quality digital audio services over lossy IP networks.

  20. Traffic Measurement for Traffic Engineering in IP/MPLS Based Telecom Core Networks

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Telecommunication networks will become IP-based networks with all voice, data and multimedia traffic carried as packets with appropriate quality and class of service for each traffic type. It is very important for service provider to apply Traffic Engineering (TE) with the dramatic growth of networks traffic. In this paper, we discuss traffic engineering based on IP/MPLS and emphasize traffic measurement in telecom core networks. We specify the technology and scientific principles of the measurement, modeling, characterization, and the traffic flow matrix. The paper concludes with a preliminary analysis of TE measurement and a discussion about the development of TE measurement.

  1. An Accurate Approach to Large-Scale IP Traffic Matrix Estimation

    Science.gov (United States)

    Jiang, Dingde; Hu, Guangmin

    This letter proposes a novel method of large-scale IP traffic matrix (TM) estimation, called algebraic reconstruction technique inference (ARTI), which is based on the partial flow measurement and Fratar model. In contrast to previous methods, ARTI can accurately capture the spatio-temporal correlations of TM. Moreover, ARTI is computationally simple since it uses the algebraic reconstruction technique. We use the real data from the Abilene network to validate ARTI. Simulation results show that ARTI can accurately estimate large-scale IP TM and track its dynamics.

  2. An integrable case of the p + ip pairing Hamiltonian interacting with its environment

    Science.gov (United States)

    Lukyanenko, Inna; Isaac, Phillip S.; Links, Jon

    2016-02-01

    We consider a generalization of the p + ip pairing Hamiltonian, with external interaction terms of a particular form. These terms allow for the exchange of particles between the system and its environment. As a result the {u}(1) symmetry associated with conservation of particle number, present in the p + ip Hamiltonian, is broken. Nonetheless the generalized model is integrable. We establish integrability using the boundary quantum inverse scattering method, with one of the reflection matrices chosen to be non-diagonal. We also derive the corresponding Bethe ansatz equations, the roots of which parametrize the exact solution for the energy spectrum.

  3. Considerations on VoIP Throughput in 802.11 Networks

    Directory of Open Access Journals (Sweden)

    POTORAC, A. D.

    2009-10-01

    Full Text Available Voice data packets have to arrive at the destination in time, with a defined cadence and with low and constant delay in order to allow the real time voice reconstruction. From this point of view, transmitting voice over IP networks is the most sensitive category of applications, especially when wireless medium is involved. The paper discusses the possibilities of transmitting the maximum number of simultaneous voice streams over 802.11 wireless networks considering the main factors which impact with VoIP throughput, in a basic scenario. Starting from a proposed communication model, the number of simultaneous possible VoIP sessions is calculated, taking into consideration the contribution of the protocol overheads, the security overheads, the PHY level timings and the CODEC proprieties. Numerical results are generated and compared.

  4. Pheromone-Based Ant Routing System for IP Networks

    Institute of Scientific and Technical Information of China (English)

    张林; 任勇; 山秀明

    2004-01-01

    The pheromone-based ant routing algorithm is a distributed routing algorithm with good scalability and robustness. A 2-D cellular automata (CA) model of the computer network was presented to analyze the algorithm. The results show that the procedure of establishing a stable route is self-organized towards the attractive peculiar state, and the duration of time for the routing establishment is power-law distributed. A practical ant routing protocol over an IP network was also presented, and two simulations were done to compare the performance dynamic and the load balancing performance between this protocol and the open shortest path first (OSPF) protocol. The results show that the ant routing protocol out-performs OSPF in these aspects.

  5. Optimal design of an IP/MPLS over DWDM network

    Directory of Open Access Journals (Sweden)

    Eduardo Canale

    2014-04-01

    Full Text Available Different approaches for deploying resilient optical networks of low cost constitute a traditional group of NP-Hard problems that have been widely studied. Most of them are based on the construction of low cost networks that fulfill connectivity constraints. However, recent trends to virtualize optical networks over the legacy fiber infrastructure, modified the nature of network design problems and turned inappropriate many of these models and algorithms. In this paper we study a design problem arising from the deployment of an IP/MPLS network over an existing DWDM infrastructure. Besides cost and resiliency, this problem integrates traffic and capacity constraints. We present: an integer programming formulation for the problem, theoretical results, and describe how several metaheuristics were applied in order to find good quality solutions, for a real application case of a telecommunications company.

  6. Matching references with MEDLINE via TCP/IP.

    Science.gov (United States)

    Guidi, J N

    1993-01-01

    Bibliographic references are an important part of databases and information resources used by clinicians and biomedical researchers. In addition to the obvious clerical advantages of standard references, bibliographic references can also be used as links to related items in different data sets. This paper describes an effort that involved matching references from a variety of disparate databases to canonical MEDLINE references. The references matched were those involved in a database unification project which is part of the Mouse Genome Informatics effort at The Jackson Laboratory. Software was developed to take advantage of a commercially available retrieval engine which accesses MEDLINE on CD-ROM disks. The software permits client programs on UNIX/C, and potentially other environments, to access unabridged MEDLINE via networks supporting the TCP/IP protocols. The matching process described can be used as a model for similar efforts with different research or clinical data sets, as well as different hardware or software environments.

  7. Computational Methods for ChIP-seq Data Analysis and Applications

    KAUST Repository

    Ashoor, Haitham

    2017-04-25

    integrates several experimental data including ChIP-seq data for TF binding sites. Finally, I present an extensive computational comparison of different ab-initio motif identification methods based on TF ChIP-seq data. The comparison included 10 different methods over 159 different TF datasets. Recommendations of this comparison indicate that the usage of simple methods outperforms the usage of high order models.

  8. Macrocell Builder: IP-Block-Based Design Environment for High-Throughput VLSI Dedicated Digital Signal Processing Systems

    Directory of Open Access Journals (Sweden)

    Urard Pascal

    2006-01-01

    Full Text Available We propose an efficient IP-block-based design environment for high-throughput VLSI systems. The flow generates SystemC register-transfer-level (RTL architecture, starting from a Matlab functional model described as a netlist of functional IP. The refinement model inserts automatically control structures to manage delays induced by the use of RTL IPs. It also inserts a control structure to coordinate the execution of parallel clocked IP. The delays may be managed by registers or by counters included in the control structure. The flow has been used successfully in three real-world DSP systems. The experimentations show that the approach can produce efficient RTL architecture and allows to save huge amount of time.

  9. A Reactive and Cycle-True IP Emulator for MPSoC Exploration

    DEFF Research Database (Denmark)

    Mahadevan, Shankar; Angiolini, Federico; Sparsø, Jens

    2008-01-01

    The design of MultiProcessor Systems-on-Chip (MPSoC) emphasizes intellectual-property (IP)-based communication-centric approaches. Therefore, for the optimization of the MPSoC interconnect, the designer must develop traffic models that realistically capture the application behavior as executing o...... of the RIPE in a design-space-exploration context....

  10. Working with mental health problems : clients' experiences of IPS, vocational rehabilitation and employment

    NARCIS (Netherlands)

    Koletsi, Marsha; Niersman, Astrid; van Busschbach, Jooske T.; Catty, Jocelyn; Becker, Thomas; Burns, Tom; Fioritti, Angelo; Kalkan, Rana; Lauber, Christoph; Roessler, Wulf; Tomov, Toma; Wiersma, Durk

    2009-01-01

    Background Although the effectiveness of individual placement and support (IPS) has been well established, little is known about clients' perceptions of the model compared to usual vocational rehabilitation, nor about their experiences of searching for and returning to work with this kind of

  11. Working with mental health problems : clients' experiences of IPS, vocational rehabilitation and employment

    NARCIS (Netherlands)

    Koletsi, Marsha; Niersman, Astrid; van Busschbach, Jooske T.; Catty, Jocelyn; Becker, Thomas; Burns, Tom; Fioritti, Angelo; Kalkan, Rana; Lauber, Christoph; Roessler, Wulf; Tomov, Toma; Wiersma, Durk

    2009-01-01

    Background Although the effectiveness of individual placement and support (IPS) has been well established, little is known about clients' perceptions of the model compared to usual vocational rehabilitation, nor about their experiences of searching for and returning to work with this kind of support

  12. [Analysis of Musculoskeletal Systems and Their Diseases. Use of iPS cells in regenerative therapy and drug discovery for cartilage diseases].

    Science.gov (United States)

    Tsumaki, Noriyuki

    2015-08-01

    Induced pluripotent stem (iPS) cells can be generated by transiently expressing defined factors in somatic cells such as dermal fibroblasts and blood cells and culturing them in specific medium. iPS cells are expected to provide new tools for research and development of therapies for various diseases because of two important properties : 1) they can be differentiated into any type of somatic cell (pluripotency) and 2) they can be expanded infinitely (self renew). Research for the transplantation of iPS cell-derived cartilage/chondrocytes and drug discovery by iPS cell-based disease modeling are ongoing.

  13. PENGEMBANGAN PERANGKAT PEMBELAJARAN IPS DENGAN PENDEKATAN INQUIRY

    Directory of Open Access Journals (Sweden)

    Lisna Handayani

    2015-08-01

    Full Text Available Tujuan  penelitian adalah menghasilkan perangkat pembelajaran IPS dengan Pendekatan Inquiry. Penelitian pengembangan ini menggunakan langkah-langkah Borg and Gall yang terdiri dari tiga tahapan utama yaitu pendahuluan, pengembangan dan validasi. Data yang diperoleh adalah hasil belajar, aktivitas diskusi dan respon peserta didik. Data dianalisis menggunakan analisis deskriptif dan uji t. Penggunaan pendekatan inquiry sebagai salah satau strategi pembelajaran IPS dapat dilakukan dengan mempersiapkan terlebih dahulu perangkat pembelajarannya. Hasil pengembangan divalidasi oleh pakar, diujicobakan pada kelas terbatas dan selanjutnya dilakukan ujicoba pada kelas eksperimen yang dibandingkan hasilnya dengan kelas kontrol. Perangkat pembelajaran tergolong efektif untuk meningkatkan hasil belajar peserta didik di kelas VII SMP Negeri 2 Jepara, terbukti dari adanya peningkatan yang nyata terbukti dari hasil uji paired sampel t-test dengan nilai signifikansi 0,000 <0,05, dan terjadi peningkatan yang tinggi (gain > 0,7, serta hasil belajar yang dicapai berbeda secara signifikan dengan kelompok kontrol. Di samping hasil belajar, pembelajaran yang dilakukan juga berdampak pada proses belajar yang membawa tingginya aktivitas peserta didik dalam memberikan gagasan, kerjasama, inisiatif, keaktifan dan kedisiplinan. The purpose of this research is creating Social Lesson Material with Inquiry Approach.This developing of the research is used of  the steps of Borg and Gall. They are Introducing, developing and evaluating. Learning materials are developed and validity by expert. Datas are gooten by study result, discussion activity and students, respone. Analizing datas are used descriptive analize and t- try out. The use of inquiry approach as one of learning strategy on Social Lesson Material can be done by preparing those. The result of  developing are being validity by expert,  try out on limited group and experiment group ad compare with control group

  14. Special rights for the development of Indigenous Peoples (IPS) in ...

    African Journals Online (AJOL)

    Special rights for the development of Indigenous Peoples (IPS) in Africa: Any need in a democratic society with ... Journal Home > Vol 4 (2013) > ... Globalization and efforts to link human, cultural and social rights to the IPs remain blurred and ...

  15. Field Test Results on Natural Field IP Method

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    In this paper the authors propose the natural field induced polarization (IP) method and present the way to pick up IP effect. The relations between the object and anomaly are studied by taking field experiments as examples. The effectiveness and usability of the method are testified.

  16. Fair Scheduling for Delay-Sensitive VoIP Traffic

    Science.gov (United States)

    Ahmed, Shawish; Jiang, Xiaohong; Horiguchi, Susumu

    With the wide expansion of voice services over the IP networks (VoIP), the volume of this delay sensitive traffic is steadily growing. The current packet schedulers for IP networks meet the delay constraint of VoIP traffic by simply assigning its packets the highest priority. This technique is acceptable as long as the amount of VoIP traffic is relatively very small compared to other non-voice traffic. With the notable expansion of VoIP applications, however, the current packet schedulers will significantly sacrifice the fairness deserved by the non-voice traffic. In this paper, we extend the conventional Deficit Round-Robin (DRR) scheduler by including a packet classifier, a Token Bucket and a resource reservation scheme and propose an integrated packet scheduler architecture for the growing VoIP traffic. We demonstrate through both theoretical analysis and extensive simulation that the new architecture makes it possible for us to significantly improve the fairness to non-voice traffic while still meeting the tight delay requirement of VoIP applications.

  17. In silico pooling of ChIP-seq control experiments.

    Science.gov (United States)

    Sun, Guannan; Srinivasan, Rajini; Lopez-Anido, Camila; Hung, Holly A; Svaren, John; Keleş, Sündüz

    2014-01-01

    As next generation sequencing technologies are becoming more economical, large-scale ChIP-seq studies are enabling the investigation of the roles of transcription factor binding and epigenome on phenotypic variation. Studying such variation requires individual level ChIP-seq experiments. Standard designs for ChIP-seq experiments employ a paired control per ChIP-seq sample. Genomic coverage for control experiments is often sacrificed to increase the resources for ChIP samples. However, the quality of ChIP-enriched regions identifiable from a ChIP-seq experiment depends on the quality and the coverage of the control experiments. Insufficient coverage leads to loss of power in detecting enrichment. We investigate the effect of in silico pooling of control samples within multiple biological replicates, multiple treatment conditions, and multiple cell lines and tissues across multiple datasets with varying levels of genomic coverage. Our computational studies suggest guidelines for performing in silico pooling of control experiments. Using vast amounts of ENCODE data, we show that pairwise correlations between control samples originating from multiple biological replicates, treatments, and cell lines/tissues can be grouped into two classes representing whether or not in silico pooling leads to power gain in detecting enrichment between the ChIP and the control samples. Our findings have important implications for multiplexing samples.

  18. Last Tribute to Professor Zheng Chengsi, Renowned IP Law Scholar

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    On 10 September 2006, Professor Zheng Chengsi, the renowned Chinese IP law scholar, Chairman of the IP Law Society of the China Law Society, Vice-President of the Copyright Society of China, and member of the Chinese Academy of Social Sciences, passed away in Beijing at the age of 62.

  19. IP-10 release assays in the diagnosis of tuberculosis infection

    DEFF Research Database (Denmark)

    Ruhwald, Morten; Aabye, Martine G; Ravn, Pernille

    2012-01-01

    The current state-of-the-art tests for infection with Mycobacterium tuberculosis - the IFN-γ release assays - rely on accurate measurement of the cytokine IFN-γ. Many other potential biomarkers are expressed in concert with IFN-γ, and IP-10 in particular has shown promising results. IP-10...

  20. Probabilistic Route Selection Algorithm for IP Traceback

    Science.gov (United States)

    Yim, Hong-Bin; Jung, Jae-Il

    DoS(Denial of Service) or DDoS(Distributed DoS) attack is a major threaten and the most difficult problem to solve among many attacks. Moreover, it is very difficult to find a real origin of attackers because DoS/DDoS attacker uses spoofed IP addresses. To solve this problem, we propose a probabilistic route selection traceback algorithm, namely PRST, to trace the attacker's real origin. This algorithm uses two types of packets such as an agent packet and a reply agent packet. The agent packet is in use to find the attacker's real origin and the reply agent packet is in use to notify to a victim that the agent packet is reached the edge router of the attacker. After attacks occur, the victim generates the agent packet and sends it to a victim's edge router. The attacker's edge router received the agent packet generates the reply agent packet and send it to the victim. The agent packet and the reply agent packet is forwarded refer to probabilistic packet forwarding table (PPFT) by routers. The PRST algorithm runs on the distributed routers and PPFT is stored and managed by routers. We validate PRST algorithm by using mathematical approach based on Poisson distribution.

  1. Performance Comparison of IP-Networked Storage

    Institute of Scientific and Technical Information of China (English)

    JU Dapeng; LIU Chuanyi; WANG Dongsheng; LIU Hong; TANG Zhizhong

    2009-01-01

    Dramatically increasing amounts of digital data are placing huge requirements on storage systems.IP-networked storage systems,such as the network file system (NFS)-based network-attached storage (NAS) systems and the iSCSI-storage area network (SAN) systems,have become increasingly common in today's local area network (LAN) environments.The emergence of new storage techniques,such as object-based storage (OBS) and content aware storage (CAS),significantly improves the functionality of storage devices to meet further needs for storage sub-systems.However,these may impact system performance.This paper compares the performance of NFS,iSCSI storage,object-based storage devices (OSDs),and CAS-based storage systems in an environment with no data sharing across host machines.A gigabit ethernet network is used as the storage network.Test results demonstrate that the performances of these systems are comparable with CAS being much better than the others for write operations.The performance bottlenecks in these systems are analyzed to provide insight into how future storage systems may be improved and possible optimization methods.The analysis shows how the I/O interfaces in these systems affect the application performance and that network-based storage systems require optimized I/O latency and reduced network and buffer processing in the servers.

  2. Perfection of Recent Attacks using IP

    Directory of Open Access Journals (Sweden)

    A. RENGARAJAN

    2012-02-01

    Full Text Available The Internet threat monitoring (ITM systems have been deployed to detect widespread attacks on the Internet in recent years. However, the effectiveness of ITM systems critically depends on the confidentiality of the location of their monitors. If adversaries learn the monitor locations of an ITM system, they can bypass the monitors and focus on the uncovered IP address space without being detected. In this paper, we study a new class of attacks, the invisible LOCalization (iLOC attack. The iLOC attack can accurately and invisibly localize monitors of ITM systems. In the iLOC attack, the attacker launches low-rate port-scan traffic, encoded with a selected pseudo noise code (PN-code, to targeted networks. While the secret PN-code is invisible to others, the attacker can accurately determine the existence of monitors in the targeted networks based on whether the PN-code is embedded in the report data queried from the data center of the ITM system. We formally analyze the impact of various parameters on attack effectiveness. We implement the iLOC attack and conduct the performance evaluation on a real-world ITM system to demonstrate the possibility of such attacks. We also conduct extensive simulations on the iLOC attack using real-world traces. Our data show that the iLOC attack can accurately identify monitors while being invisible to ITM systems. Finally, we present a set of guidelines to counteract the iLOC attack.

  3. Quantification of histone modification ChIP-seq enrichment for data mining and machine learning applications

    Directory of Open Access Journals (Sweden)

    Bekiranov Stefan

    2011-08-01

    Full Text Available Abstract Background The advent of ChIP-seq technology has made the investigation of epigenetic regulatory networks a computationally tractable problem. Several groups have applied statistical computing methods to ChIP-seq datasets to gain insight into the epigenetic regulation of transcription. However, methods for estimating enrichment levels in ChIP-seq data for these computational studies are understudied and variable. Since the conclusions drawn from these data mining and machine learning applications strongly depend on the enrichment level inputs, a comparison of estimation methods with respect to the performance of statistical models should be made. Results Various methods were used to estimate the gene-wise ChIP-seq enrichment levels for 20 histone methylations and the histone variant H2A.Z. The Multivariate Adaptive Regression Splines (MARS algorithm was applied for each estimation method using the estimation of enrichment levels as predictors and gene expression levels as responses. The methods used to estimate enrichment levels included tag counting and model-based methods that were applied to whole genes and specific gene regions. These methods were also applied to various sizes of estimation windows. The MARS model performance was assessed with the Generalized Cross-Validation Score (GCV. We determined that model-based methods of enrichment estimation that spatially weight enrichment based on average patterns provided an improvement over tag counting methods. Also, methods that included information across the entire gene body provided improvement over methods that focus on a specific sub-region of the gene (e.g., the 5' or 3' region. Conclusion The performance of data mining and machine learning methods when applied to histone modification ChIP-seq data can be improved by using data across the entire gene body, and incorporating the spatial distribution of enrichment. Refinement of enrichment estimation ultimately improved accuracy

  4. EFFICIENT DEFENSE SYSTEM FOR IP SPOOFING IN NETWORKS

    Directory of Open Access Journals (Sweden)

    Emil Kuriakose John

    2012-10-01

    Full Text Available In this age of gigabit Ethernet and broadband internet, network security has been the top priority for most of the researchers. Technology advancements have advantages as well as disadvantages. Most of the communication of present world, the e-world, takes place online, through the internet. Thus the context of network intrusions and attacks to hack into servers also came into existence. A technique to perform this activity is made possible by preventing the discovery of the sender’s identity through IP Spoofing [7]. Many popular internet sites have been hacked and attackers try to forge or spoof the source addresses in IP packets. Using spoofing detection technique, the user can retrieve the list of IP addresses and able to identify the malicious IP addresses.Hence mechanisms must be designed to prevent hacking. This paper proposes a novel technique to detect IP spoofing based on traffic verification and filtering.

  5. Audio CAPTCHA for SIP-Based VoIP

    Science.gov (United States)

    Soupionis, Yannis; Tountas, George; Gritzalis, Dimitris

    Voice over IP (VoIP) introduces new ways of communication, while utilizing existing data networks to provide inexpensive voice communications worldwide as a promising alternative to the traditional PSTN telephony. SPam over Internet Telephony (SPIT) is one potential source of future annoyance in VoIP. A common way to launch a SPIT attack is the use of an automated procedure (bot), which generates calls and produces audio advertisements. In this paper, our goal is to design appropriate CAPTCHA to fight such bots. We focus on and develop audio CAPTCHA, as the audio format is more suitable for VoIP environments and we implement it in a SIP-based VoIP environment. Furthermore, we suggest and evaluate the specific attributes that audio CAPTCHA should incorporate in order to be effective, and test it against an open source bot implementation.

  6. COPAR: A ChIP-Seq Optimal Peak Analyzer

    Directory of Open Access Journals (Sweden)

    Binhua Tang

    2017-01-01

    Full Text Available Sequencing data quality and peak alignment efficiency of ChIP-sequencing profiles are directly related to the reliability and reproducibility of NGS experiments. Till now, there is no tool specifically designed for optimal peak alignment estimation and quality-related genomic feature extraction for ChIP-sequencing profiles. We developed open-sourced COPAR, a user-friendly package, to statistically investigate, quantify, and visualize the optimal peak alignment and inherent genomic features using ChIP-seq data from NGS experiments. It provides a versatile perspective for biologists to perform quality-check for high-throughput experiments and optimize their experiment design. The package COPAR can process mapped ChIP-seq read file in BED format and output statistically sound results for multiple high-throughput experiments. Together with three public ChIP-seq data sets verified with the developed package, we have deposited COPAR on GitHub under a GNU GPL license.

  7. Inositol hexakisphosphate (IP6) generated by IP5K mediates cullin-COP9 signalosome interactions and CRL function.

    Science.gov (United States)

    Scherer, Paul C; Ding, Yan; Liu, Zhiqing; Xu, Jing; Mao, Haibin; Barrow, James C; Wei, Ning; Zheng, Ning; Snyder, Solomon H; Rao, Feng

    2016-03-29

    The family of cullin-RING E3 Ligases (CRLs) and the constitutive photomorphogenesis 9 (COP9) signalosome (CSN) form dynamic complexes that mediate ubiquitylation of 20% of the proteome, yet regulation of their assembly/disassembly remains poorly understood. Inositol polyphosphates are highly conserved signaling molecules implicated in diverse cellular processes. We now report that inositol hexakisphosphate (IP6) is a major physiologic determinant of the CRL-CSN interface, which includes a hitherto unidentified electrostatic interaction between the N-terminal acidic tail of CSN subunit 2 (CSN2) and a conserved basic canyon on cullins. IP6, with an EC50 of 20 nM, acts as an intermolecular "glue," increasing cullin-CSN2 binding affinity by 30-fold, thereby promoting assembly of the inactive CRL-CSN complexes. The IP6 synthase, Ins(1,3,4,5,6)P5 2-kinase (IPPK/IP5K) binds to cullins. Depleting IP5K increases the percentage of neddylated, active Cul1 and Cul4A, and decreases levels of the Cul1/4A substrates p27 and p21. Besides dysregulating CRL-mediated cell proliferation and UV-induced apoptosis, IP5K depletion potentiates by 28-fold the cytotoxic effect of the neddylation inhibitor MLN4924. Thus, IP5K and IP6 are evolutionarily conserved components of the CRL-CSN system and are potential targets for cancer therapy in conjunction with MLN4924.

  8. Electric conductivity for laboratory and field monitoring of induced partial saturation (IPS) in sands

    Science.gov (United States)

    Kazemiroodsari, Hadi

    implemented in the prepared specimen to validate the numerical simulation model and explore the use of conductivity probes to detect the transport of chemical solution, estimate degree of saturation achieved due to injection of chemical solution, and evaluate final zone of partial saturation. The conductivity probe and the simulation results agreed well. To study the effect of IPS on liquefaction response of the sand specimen, IPS was implemented in a large (2-story high) sand specimen prepared in the laminar box of NEES Buffalo and then the specimen was subjected to harmonic shaking. Electric conductivity probes were used in the specimen treatment by controlling the duration and spacing of injection of the chemical solution, in monitoring the transport of chemical solution, in the estimation of zone of partial saturation achieved, and in the estimation of degree of saturation achieved due to implementation of IPS. The conductivity probes indicated partial saturation of the specimen. The shaking tests results confirmed the partial saturation state of the sand specimen. In addition, to the laboratory works, electric conductivity probes were used in field implementation of IPS in a pilot test at the Wildlife Liquefaction Array (WLA) of NEES UCSB site. The conductivity probes in the field test helped decide the optimum injection pressure, the injection tube spacing, and the degree of saturation that could be achieved in the field. The various laboratory and field tests confirmed that electric conductivity and the probes devised and used can be invaluable in the implementation of IPS, by providing information regarding transport of the chemical solution, the spacing of injection tubes, duration of injection, and the zone and degree of partial saturation caused by IPS.

  9. Quercetin increases the bioavailability of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in rats

    NARCIS (Netherlands)

    Schutte, M.E.; Alink, G.M.; Freidig, A.P.; Spenkelink, A.; Vaessen, J.; Sandt, van de J.J.M.; Groten, J.P.; Rietjens, I.M.C.M.

    2008-01-01

    This study investigates whether the previous observation that quercetin increases the transport of PhIP through Caco-2 monolayers in vitro could be confirmed in an in vivo rat model. Co-administration of 1.45 micromol PhIP/kg bw and 30 micromol quercetin/kg bw significantly increased the blood AUC(0

  10. SignalSpider: Probabilistic pattern discovery on multiple normalized ChIP-Seq signal profiles

    KAUST Repository

    Wong, Kachun

    2014-09-05

    Motivation: Chromatin immunoprecipitation (ChIP) followed by high-throughput sequencing (ChIP-Seq) measures the genome-wide occupancy of transcription factors in vivo. Different combinations of DNA-binding protein occupancies may result in a gene being expressed in different tissues or at different developmental stages. To fully understand the functions of genes, it is essential to develop probabilistic models on multiple ChIP-Seq profiles to decipher the combinatorial regulatory mechanisms by multiple transcription factors. Results: In this work, we describe a probabilistic model (SignalSpider) to decipher the combinatorial binding events of multiple transcription factors. Comparing with similar existing methods, we found SignalSpider performs better in clustering promoter and enhancer regions. Notably, SignalSpider can learn higher-order combinatorial patterns from multiple ChIP-Seq profiles. We have applied SignalSpider on the normalized ChIP-Seq profiles from the ENCODE consortium and learned model instances. We observed different higher-order enrichment and depletion patterns across sets of proteins. Those clustering patterns are supported by Gene Ontology (GO) enrichment, evolutionary conservation and chromatin interaction enrichment, offering biological insights for further focused studies. We also proposed a specific enrichment map visualization method to reveal the genome-wide transcription factor combinatorial patterns from the models built, which extend our existing fine-scale knowledge on gene regulation to a genome-wide level. Availability and implementation: The matrix-algebra-optimized executables and source codes are available at the authors\\' websites: http://www.cs.toronto.edu/∼wkc/SignalSpider. Contact: Supplementary information: Supplementary data are available at Bioinformatics online.

  11. DAB2IP 基因的研究进展%Progress on DAB2IP gene

    Institute of Scientific and Technical Information of China (English)

    袁妍; 陈海滨

    2011-01-01

    Human DAB2 interaction protein (DAB2IP) is a novel member of Ras GTPase-activating protein family. It interacts directly with disabled-2 protein (DAB2/DOC2) which suppresses growth of cancers derived from different tissues, including mammary, prostate and ovarian cancers. DAB2IP was identified as an immediate downstream effector mediated by DAB2/DOC2. DAB2IP and DAB2/DOC2 form a unique protein complex that has a negative regulatory effect on the Ras-mediated signal pathway. It is demonstrated that DAB2IP is a tumor suppressor gene inactivated by methylation in several cancers. This article reviews the structure and biological functions of DAB2IP gene as well as its potential roles in carcinogenesis and evolution.

  12. IP VPN及Internet VPN分类研究%A methodology of IP and internet virtual private network classification

    Institute of Scientific and Technical Information of China (English)

    王桐

    2009-01-01

    关于IP VPN的分类目前已经有很多研究,但目前还没有一个统一的说法,而对于Inter-net VPN的分类研究目前还没有见到.因此,PN和VPN,以及IP VPN和Internet VPN的区别和联系很容易被混淆,本文尝试给出了VPN、IP VPN及Internet VPN的一个准确定义,并在定义的基础上对IP VPN和Internet VPN提出了一种分类方法,最后给出了IP VPN和Internet VPN的比较及研究结果.

  13. IP-10 is a potential biomarker of cystic fibrosis acute pulmonary exacerbations.

    Directory of Open Access Journals (Sweden)

    George M Solomon

    Full Text Available BACKGROUND: Cystic fibrosis (CF is characterized by acute pulmonary exacerbations (APE. The CF nasal airway exhibits a similar ion transport defect as the lung, and colonization, infection, and inflammation within the nasal passages are common among CF patients. Nasal lavage fluid (NLF is a minimally invasive means to collect upper airway samples. METHODS: We collected NLF at the onset and resolution of CF APE and compared a 27-plex cytokine profile to stable CF outpatients and normal controls. We also tested IP-10 levels in the bronchoalveolar lavage fluid (BALF of CF patients. Well-differentiated murine sinonasal monolayers were exposed to bacterial stimulus, and IP-10 levels were measured to test epithelial secretion. RESULTS: Subjects hospitalized for APE had elevated IP-10 (2582 pg/mL [95% CL of mean: 818,8165], N=13 which significantly decreased (647 pg/mL [357,1174], P<0.05, N =13 following antimicrobial therapy. Stable CF outpatients exhibited intermediately elevated levels (680 pg/mL [281,1644], N=13 that were less than CF inpatients upon admission (P=0.056 but not significantly different than normal controls (342 pg/mL [110,1061]; P=0.3, N=10. IP-10 was significantly increased in CF BALF (2673 pg/mL [1306,5458], N=10 compared to healthy post-lung transplant patients (8.4 pg/mL [0.03,2172], N=5, P<0.001. IP-10 levels from well-differentiated CF murine nasal epithelial monolayers exposed to Pseudomonas PAO-1 bacteria-free prep or LPS (100 nM apically for 24 hours were significantly elevated (1159 ± 147, P<0.001 for PAO-1; 1373 ± 191, P<0.001 for LPS vs. 305 ± 68 for vehicle controls. Human sino-nasal epithelial cells derived from CF patients had a similar response to LPS (34% increase, P<0.05, N=6. CONCLUSIONS: IP-10 is elevated in the nasal lavage of CF patients with APE and responds to antimicrobial therapy. IP-10 is induced by airway epithelia following stimulation with bacterial pathogens in a murine model. Additional research

  14. HPeak: an HMM-based algorithm for defining read-enriched regions in ChIP-Seq data

    Directory of Open Access Journals (Sweden)

    Maher Christopher A

    2010-07-01

    Full Text Available Abstract Background Protein-DNA interaction constitutes a basic mechanism for the genetic regulation of target gene expression. Deciphering this mechanism has been a daunting task due to the difficulty in characterizing protein-bound DNA on a large scale. A powerful technique has recently emerged that couples chromatin immunoprecipitation (ChIP with next-generation sequencing, (ChIP-Seq. This technique provides a direct survey of the cistrom of transcription factors and other chromatin-associated proteins. In order to realize the full potential of this technique, increasingly sophisticated statistical algorithms have been developed to analyze the massive amount of data generated by this method. Results Here we introduce HPeak, a Hidden Markov model (HMM-based Peak-finding algorithm for analyzing ChIP-Seq data to identify protein-interacting genomic regions. In contrast to the majority of available ChIP-Seq analysis software packages, HPeak is a model-based approach allowing for rigorous statistical inference. This approach enables HPeak to accurately infer genomic regions enriched with sequence reads by assuming realistic probability distributions, in conjunction with a novel weighting scheme on the sequencing read coverage. Conclusions Using biologically relevant data collections, we found that HPeak showed a higher prevalence of the expected transcription factor binding motifs in ChIP-enriched sequences relative to the control sequences when compared to other currently available ChIP-Seq analysis approaches. Additionally, in comparison to the ChIP-chip assay, ChIP-Seq provides higher resolution along with improved sensitivity and specificity of binding site detection. Additional file and the HPeak program are freely available at http://www.sph.umich.edu/csg/qin/HPeak.

  15. THE CYTOKINE IP-10 IN CHRONIC HBV AND HCV INFECTION

    Directory of Open Access Journals (Sweden)

    Nina S. Nikolova

    2013-08-01

    Full Text Available Introduction: IP-10 it has been studied as a predictor of treatment response in chronic HCV infected patients. The data for the HBV infection are not enough.Aim: To compare IP-10 levels in patients with chronic HBV /CHB/ and HCV infection /CHC/ and their relation to liver disease and treatment response. Material and methods: 20 patients - with CHC genotype 1 infection /on standard bi-therapy/ and 32 patients with CHB /21 pts - NUC; 11 pts - IFN/. Results: The IP-10 did not correlate with sex, age, ALT and liver fibrosis. The basal IP-10 were lower in patients with CHB (p=0,017. There was a difference in IP-10 baseline levels among the HCV patients with or without RVR (p=0,007. A negative correlation was found between basal IP-10 and RVR (r= -0,508; p=0,008. Conclusion: IP-10 could predict virological response in patients with CHC on standard bi-therapy, but not in HBV infected patients on standard therapy.

  16. Reviewing ChIPS, The Chandra Imaging and Plotting System

    Science.gov (United States)

    Miller, J.; Burke, D. J.; Evans, I. N.; Evans, J. D.; McLaughlin, W.

    2015-09-01

    The Chandra Imaging and Plotting System (ChIPS) is a 2D plotting system designed to allow users to easily create, manipulate, and produce publication quality visualizations. ChIPS has a simple but very powerful interactive interface that allows users to dynamically modify the contents and layout of their plots quickly and efficiently, with the results of any changes being immediately visible. ChIPS allows users to construct their plots fully interactively, and then save the final plot commands as a Python script. This bypasses the need to iteratively edit and rerun the script when developing the plot. Features such as undo and redo commands allow users to easily step backwards and forwards through previous commands, while the ability so save ChIPS sessions in a platform-independent state file allows the session to be restored at any time, even on another machine. Because ChIPS offers a Python interface, users can analyze their data using the broad array of modules offered in Python, and visualize the information in ChIPS at the same time. In this paper we explore the design decisions behind the development of ChIPS and some of the lessons learned along the way.

  17. MEME-ChIP: motif analysis of large DNA datasets.

    Science.gov (United States)

    Machanick, Philip; Bailey, Timothy L

    2011-06-15

    Advances in high-throughput sequencing have resulted in rapid growth in large, high-quality datasets including those arising from transcription factor (TF) ChIP-seq experiments. While there are many existing tools for discovering TF binding site motifs in such datasets, most web-based tools cannot directly process such large datasets. The MEME-ChIP web service is designed to analyze ChIP-seq 'peak regions'--short genomic regions surrounding declared ChIP-seq 'peaks'. Given a set of genomic regions, it performs (i) ab initio motif discovery, (ii) motif enrichment analysis, (iii) motif visualization, (iv) binding affinity analysis and (v) motif identification. It runs two complementary motif discovery algorithms on the input data--MEME and DREME--and uses the motifs they discover in subsequent visualization, binding affinity and identification steps. MEME-ChIP also performs motif enrichment analysis using the AME algorithm, which can detect very low levels of enrichment of binding sites for TFs with known DNA-binding motifs. Importantly, unlike with the MEME web service, there is no restriction on the size or number of uploaded sequences, allowing very large ChIP-seq datasets to be analyzed. The analyses performed by MEME-ChIP provide the user with a varied view of the binding and regulatory activity of the ChIP-ed TF, as well as the possible involvement of other DNA-binding TFs. MEME-ChIP is available as part of the MEME Suite at http://meme.nbcr.net.

  18. On recovering distributed IP information from inductive source time domain electromagnetic data

    Science.gov (United States)

    Kang, Seogi; Oldenburg, Douglas W.

    2016-10-01

    We develop a procedure to invert time domain induced polarization (IP) data for inductive sources. Our approach is based upon the inversion methodology in conventional electrical IP (EIP), which uses a sensitivity function that is independent of time. However, significant modifications are required for inductive source IP (ISIP) because electric fields in the ground do not achieve a steady state. The time-history for these fields needs to be evaluated and then used to define approximate IP currents. The resultant data, either a magnetic field or its derivative, are evaluated through the Biot-Savart law. This forms the desired linear relationship between data and pseudo-chargeability. Our inversion procedure has three steps: (1) Obtain a 3-D background conductivity model. We advocate, where possible, that this be obtained by inverting early-time data that do not suffer significantly from IP effects. (2) Decouple IP responses embedded in the observations by forward modelling the TEM data due to a background conductivity and subtracting these from the observations. (3) Use the linearized sensitivity function to invert data at each time channel and recover pseudo-chargeability. Post-interpretation of the recovered pseudo-chargeabilities at multiple times allows recovery of intrinsic Cole-Cole parameters such as time constant and chargeability. The procedure is applicable to all inductive source survey geometries but we focus upon airborne time domain EM (ATEM) data with a coincident-loop configuration because of the distinctive negative IP signal that is observed over a chargeable body. Several assumptions are adopted to generate our linearized modelling but we systematically test the capability and accuracy of the linearization for ISIP responses arising from different conductivity structures. On test examples we show: (1) our decoupling procedure enhances the ability to extract information about existence and location of chargeable targets directly from the data maps

  19. On recovering distributed IP information from inductive source time domain electromagnetic data

    Science.gov (United States)

    Kang, Seogi; Oldenburg, Douglas W.

    2016-07-01

    We develop a procedure to invert time domain induced polarization (IP) data for inductive sources. Our approach is based upon the inversion methodology in conventional electrical IP (EIP), which uses a sensitivity function that is independent of time. However, significant modifications are required for inductive source IP (ISIP) because electric fields in the ground do not achieve a steady state. The time-history for these fields needs to be evaluated and then used to define approximate IP currents. The resultant data, either a magnetic field or its derivative, are evaluated through the Biot-Savart law. This forms the desired linear relationship between data and pseudo-chargeability. Our inversion procedure has three steps: 1) Obtain a 3D background conductivity model. We advocate, where possible, that this be obtained by inverting early-time data that do not suffer significantly from IP effects. 2) Decouple IP responses embedded in the observations by forward modelling the TEM data due to a background conductivity and subtracting these from the observations. 3) Use the linearized sensitivity function to invert data at each time channel and recover pseudo-chargeability. Post-interpretation of the recovered pseudo-chargeabilities at multiple times allows recovery of intrinsic Cole-Cole parameters such as time constant and chargeability. The procedure is applicable to all inductive source survey geometries but we focus upon airborne time domain EM (ATEM) data with a coincident-loop configuration because of the distinctive negative IP signal that is observed over a chargeable body. Several assumptions are adopted to generate our linearized modelling but we systematically test the capability and accuracy of the linearization for ISIP responses arising from different conductivity structures. On test examples we show: (a) our decoupling procedure enhances the ability to extract information about existence and location of chargeable targets directly from the data maps; (b

  20. Architecture of fast IP forwarding engine in gigabit ethernet system

    Science.gov (United States)

    Do, Han C.; Lee, Hyeong H.; Cha, Kyoon Hyun

    1999-11-01

    In recent years, Internet traffic has been increased rapidly as a result of the Internet which accommodates multimedia traffic such as IP telephony and video conference. Gigabit routing technology is one possible approach to handle such internet traffic. This paper presents an efficient IP forwarding architecture adequate for Gigabit Ethernet switching system. The presented IP forwarding architecture is based upon distributed and pipelined process, which can effectively facilitate searching, editing, traffic classification, forwarding, and traffic management in parallel. Additionally, it can also process packets at full wire-speed in the ASIC level.

  1. IP ADDRESS AUTOCONFIGURATION FOR WIRELESS AD HOC NETWORKS

    Institute of Scientific and Technical Information of China (English)

    阿姆贾德

    2002-01-01

    A novel mechanism was specified by which a node in ad hoc network may autoconfigure an IP address which is unique throughout the mobile ad hoc network. This new algorithm imposes less and constant overhead and delay in obtaining an IP address, and fully utilizes the available addresses space of an ad hoc network, and independent of the existing routing protocol, and less prone to security threats. Moreover, a new Join/Leave mechanism was proposed as an enhancement to the new IP address autoconfiguration algorithm, to support the overall operation of the existing routing protocol of wireless ad hoc networks.

  2. UTN VoIP test bed (Voice over Internet Protocol)

    OpenAIRE

    Clérigo, Patricia; Mercado, Gustavo; Lima, Armando; Gosetto, Gerardo

    2009-01-01

    La tecnología Voice over IP permite transmitir paquetes de voz usando los protocolos de Internet. Esto es el basamento de la convergencia de video, voz y datos en una sola red y bajo el mismo protocolo; metodología que promete confiabilidad, accesibilidad y por sobre todo bajos costos. La UTN VoIP es un servicio de Telefonía que usa los protocolos VoIP de Internet y es montado sobre la Red Universitaria Tecnológica II. Cuando esté implementada conectará todas las Facultades Regionales y la Un...

  3. A Novel Smart Meter Controlling System with Dynamic IP Addresses

    DEFF Research Database (Denmark)

    Manembu, Pinrolinvic; Welang, Brammy; Kalua Lapu, Aditya

    2017-01-01

    Smart meters are the electronic devices for measuring energy consumption in real time. Usually, static public IP addresses are allocated to realize the point-to-point (P2P) communication and remote controlling for smart metering systems. This, however, restricts the wide deployment of smart meters......, due to the deficiency of public IP resources. This paper proposes a novel subscription-based communication architecture for the support of dynamic IP addresses and group controlling of smart meters. The paper evaluates the proposed architecture by comparing the traditional P2P architecture......, and validate its effectiveness to interact with smart meters....

  4. Estimating the geoeffectiveness of halo CMEs from associated solar and IP parameters using neural networks

    Directory of Open Access Journals (Sweden)

    J. Uwamahoro

    2012-06-01

    Full Text Available Estimating the geoeffectiveness of solar events is of significant importance for space weather modelling and prediction. This paper describes the development of a neural network-based model for estimating the probability occurrence of geomagnetic storms following halo coronal mass ejection (CME and related interplanetary (IP events. This model incorporates both solar and IP variable inputs that characterize geoeffective halo CMEs. Solar inputs include numeric values of the halo CME angular width (AW, the CME speed (Vcme, and the comprehensive flare index (cfi, which represents the flaring activity associated with halo CMEs. IP parameters used as inputs are the numeric peak values of the solar wind speed (Vsw and the southward Z-component of the interplanetary magnetic field (IMF or Bs. IP inputs were considered within a 5-day time window after a halo CME eruption. The neural network (NN model training and testing data sets were constructed based on 1202 halo CMEs (both full and partial halo and their properties observed between 1997 and 2006. The performance of the developed NN model was tested using a validation data set (not part of the training data set covering the years 2000 and 2005. Under the condition of halo CME occurrence, this model could capture 100% of the subsequent intense geomagnetic storms (Dst ≤ −100 nT. For moderate storms (−100 < Dst ≤ −50, the model is successful up to 75%. This model's estimate of the storm occurrence rate from halo CMEs is estimated at a probability of 86%.

  5. A New Wireless VoIP Signaling Device Supporting SIP and H.323 Protocols

    Directory of Open Access Journals (Sweden)

    Salma Rattal

    2014-01-01

    Full Text Available Nowadays, VoIP is a technology with a great demand and wireless networks are increasingly deployed. Each of these has its own technology constraints. For VoIP, it is very important to take into consideration the need to provide a high quality service according to well-defined standard transmission (jitter, end-to-end delay, MOS, and packet loss. However, wireless networks (IEEE 802.11 are based on radio which undergoes a number of technical constraints to achieve theoretical transmission rates; among these constraints the number of users of the networks, the distance between the client and the access, and the amount of data transmitted point are included. In this term, a study is made by simulating wireless network in OPNET Modeler with a fairly large number of VoIPs (15 users whose signaling is handled via a new node that was created specifically to manage the signaling tasks under SIP and H.323 in order to minimize the number of nodes in the network and avoid the congestion. In this paper, two scenarios are compared; the first contains a number of VoIP users with SIP and H.323 signaling handled by the new created device; the second scenario is similar to the first except that the distance between the stations is remarkably lower.

  6. COMPARATIVE ANALYISIS ON SOME POSSIBLE PARTNERSHIP SCHEMES OF GLOBAL IP EXCHANGE PROVIDERS

    Directory of Open Access Journals (Sweden)

    David Gunawan

    2014-03-01

    Full Text Available IPX (IP eXchange is GSMA’s proposal for IP interconnection model which supports multi services to offer end-to-end QoS, security, interoperability, SLAs through a dedicated connection. It provides a commercial and technical solution to manage IP traffic and follows the GSMA’s 4 key IP interworking principle such as openness, quality, cascading payments, and efficient connectivity. In order to get global IPX reachability, it is possible for an IPX provider to build partnership with other global IPX providers in business and network configuration. There are some possible partnership schemes between IPX providers such as peering mode, semi-hosted mode, full-hosted mode, or combination between these modes. The implementation of the schemes will be case-by-case basis with some considerations based on (but not limited to IPX Provider’s network asset & coverage, services & features offer, commercial offer, and customers. For an IPX provider to become competitive in IPX business and become a global IPX hub, some value added should be considered such as cost efficiency and great network performance. To achieve it, an IPX provider could implement some strategies such as build network sinergy between them and partners to develop IPX Service as single offering, offer their customers with bundled access network and services. An IPX provider should also consider their existing customer-based that can be a benefit to their bargaining position to other potential IPX provider partners to determine price and business scheme for partnership.

  7. PEMBELAJARAN IPS DALAM REALITA DI ERA KTSP: STUDI EKSPLORASI PELAKSANAAN PEMBELAJARAN IPS PADA JENJANG SMP DI KABUPATEN PATI

    Directory of Open Access Journals (Sweden)

    Edi Sutrisna

    2011-10-01

    Full Text Available Application of KTSP as a curriculum based on competency requires the implementation of strategies and methods that can deliver a number of learners achieving a particular competence. IPS as a subject who has a noble purpose, namely to prepare students to be good citizens, should be taught to students through appropriate strategies and methods by utilizing various media sources and learning. Most social studies teachers still promote the use of expository strategies in presenting lessons of Social Science education and the use of resources and learning media are minimal. Environment, as a laboratory of IPS is not utilized properly.The study shows that most teachers still tend to use expository teaching strategies, use of resources and learning media that are less varied, and integrated approaches to teaching social studies can not be realized by the teachers due to various constraints.   Keywords: learning, IPS, junior school, KTSP   Penerapan KTSP sebagai kurikulum berbasis kompetensi membutuhkan penerapan strategi dan metode yang dapat memberikan sejumlah peserta didik mencapai kompetensi tertentu. IPS sebagai subjek yang memiliki tujuan mulia, yaitu untuk mempersiapkan siswa untuk menjadi warga negara yang baik, harus diajarkan kepada siswa melalui strategi yang tepat dan metode dengan memanfaatkan berbagai sumber media dan pembelajaran. Kebanyakan guru IPS masih menggunakan strategi ekspositori dalam menyajikan meteri pelajaran IPS dengan menggunakan sumber daya dan media pembelajaran yang minimal. Lingkungan sekitar, sebagai laboratorium IPS tidak digunakan menunjukkan pembelajaran yang baik. Kebanyakan guru masih cenderung untuk menggunakan strategi pengajaran ekspositori, penggunaan sumber daya dan media pembelajaran yang kurang bervariasi, dan pendekatan terpadu untuk mengajar IPS, sehingga tidak dapat direalisasikan oleh para guru karena berbagai kendala.   Kata kunci: pembelajaran, IPS, SMP, KTSP  

  8. Non-viral generation of marmoset monkey iPS cells by a six-factor-in-one-vector approach.

    Directory of Open Access Journals (Sweden)

    Katharina Debowski

    Full Text Available Groundbreaking studies showed that differentiated somatic cells of mouse and human origin could be reverted to a stable pluripotent state by the ectopic expression of only four proteins. The resulting pluripotent cells, called induced pluripotent stem (iPS cells, could be an alternative to embryonic stem cells, which are under continuous ethical debate. Hence, iPS cell-derived functional cells such as neurons may become the key for an effective treatment of currently incurable degenerative diseases. However, besides the requirement of efficacy testing of the therapy also its long-term safety needs to be carefully evaluated in settings mirroring the clinical situation in an optimal way. In this context, we chose the long-lived common marmoset monkey (Callithrix jacchus as a non-human primate species to generate iPS cells. The marmoset monkey is frequently used in biomedical research and is gaining more and more preclinical relevance due to the increasing number of disease models. Here, we describe, to our knowledge, the first-time generation of marmoset monkey iPS cells from postnatal skin fibroblasts by non-viral means. We used the transposon-based, fully reversible piggyback system. We cloned the marmoset monkey reprogramming factors and established robust and reproducible reprogramming protocols with a six-factor-in-one-construct approach. We generated six individual iPS cell lines and characterized them in comparison with marmoset monkey embryonic stem cells. The generated iPS cells are morphologically indistinguishable from marmoset ES cells. The iPS cells are fully reprogrammed as demonstrated by differentiation assays, pluripotency marker expression and transcriptome analysis. They are stable for numerous passages (more than 80 and exhibit euploidy. In summary, we have established efficient non-viral reprogramming protocols for the derivation of stable marmoset monkey iPS cells, which can be used to develop and test cell replacement

  9. Non-viral generation of marmoset monkey iPS cells by a six-factor-in-one-vector approach.

    Science.gov (United States)

    Debowski, Katharina; Warthemann, Rita; Lentes, Jana; Salinas-Riester, Gabriela; Dressel, Ralf; Langenstroth, Daniel; Gromoll, Jörg; Sasaki, Erika; Behr, Rüdiger

    2015-01-01

    Groundbreaking studies showed that differentiated somatic cells of mouse and human origin could be reverted to a stable pluripotent state by the ectopic expression of only four proteins. The resulting pluripotent cells, called induced pluripotent stem (iPS) cells, could be an alternative to embryonic stem cells, which are under continuous ethical debate. Hence, iPS cell-derived functional cells such as neurons may become the key for an effective treatment of currently incurable degenerative diseases. However, besides the requirement of efficacy testing of the therapy also its long-term safety needs to be carefully evaluated in settings mirroring the clinical situation in an optimal way. In this context, we chose the long-lived common marmoset monkey (Callithrix jacchus) as a non-human primate species to generate iPS cells. The marmoset monkey is frequently used in biomedical research and is gaining more and more preclinical relevance due to the increasing number of disease models. Here, we describe, to our knowledge, the first-time generation of marmoset monkey iPS cells from postnatal skin fibroblasts by non-viral means. We used the transposon-based, fully reversible piggyback system. We cloned the marmoset monkey reprogramming factors and established robust and reproducible reprogramming protocols with a six-factor-in-one-construct approach. We generated six individual iPS cell lines and characterized them in comparison with marmoset monkey embryonic stem cells. The generated iPS cells are morphologically indistinguishable from marmoset ES cells. The iPS cells are fully reprogrammed as demonstrated by differentiation assays, pluripotency marker expression and transcriptome analysis. They are stable for numerous passages (more than 80) and exhibit euploidy. In summary, we have established efficient non-viral reprogramming protocols for the derivation of stable marmoset monkey iPS cells, which can be used to develop and test cell replacement therapies in

  10. MOBE-ChIP: a large-scale chromatin immunoprecipitation assay for cell type-specific studies.

    Science.gov (United States)

    Lau, On Sun; Bergmann, Dominique C

    2015-10-01

    Cell type-specific transcriptional regulators play critical roles in the generation and maintenance of multicellularity. As they are often expressed at low levels, in vivo DNA-binding studies of these regulators by standard chromatin immunoprecipitation (ChIP) assays are technically challenging. We describe here an optimized ChIP protocol termed Maximized Objects for Better Enrichment (MOBE)-ChIP, which enhances the sensitivity of ChIP assays for detecting cell type-specific signals. The protocol, which is based on the disproportional increase of target signals over background at higher scales, uses substantially greater volume of starting materials than conventional ChIPs to achieve high signal enrichment. This technique can capture weak binding events that are ambiguous in standard ChIP assays, and is useful both in gene-specific and whole-genome analysis. This protocol has been optimized for Arabidopsis, but should be applicable to other model systems with minor modifications. The full procedure can be completed within 3 days.

  11. Efficient Integration of Pipelined IP Blocks into Automatically Compiled Datapaths

    Directory of Open Access Journals (Sweden)

    Andreas Koch

    2006-12-01

    Full Text Available Compilers for reconfigurable computers aim to generate problem-specific optimized datapaths for kernels extracted from an input language. In many cases, however, judicious use of preexisting manually optimized IP blocks within these datapaths could improve the compute performance even further. The integration of IP blocks into the compiled datapaths poses a different set of problems than stitching together IPs to form a system-on-chip; though, instead of the loose coupling using standard busses employed by SoCs, the one between datapath and IP block must be much tighter. To this end, we propose a concise language that can be efficiently synthesized using a template-based approach for automatically generating lightweight data and control interfaces at the datapath level.

  12. Efficient Integration of Pipelined IP Blocks into Automatically Compiled Datapaths

    Directory of Open Access Journals (Sweden)

    Koch Andreas

    2007-01-01

    Full Text Available Compilers for reconfigurable computers aim to generate problem-specific optimized datapaths for kernels extracted from an input language. In many cases, however, judicious use of preexisting manually optimized IP blocks within these datapaths could improve the compute performance even further. The integration of IP blocks into the compiled datapaths poses a different set of problems than stitching together IPs to form a system-on-chip; though, instead of the loose coupling using standard busses employed by SoCs, the one between datapath and IP block must be much tighter. To this end, we propose a concise language that can be efficiently synthesized using a template-based approach for automatically generating lightweight data and control interfaces at the datapath level.

  13. HST/FOS Eclipse mapping of IP Pegasi in outburst

    CERN Document Server

    Saitô, R; Horne, K

    2004-01-01

    We report the results of a time-resolved eclipse mapping of the dwarf nova IP Pegasi during the decline of its May 1993 outburst from HST/FOS fast spectroscopy covering 3 eclipses in the ultraviolet spectral range.

  14. Apolipoprotein E gene knock-out and high-fat diet on IP3 and IP3R-1 expression in neurons of mice hippocampal CA1 and CA3%载脂蛋白E基因敲除及高脂饮食小鼠海马CA1和CA3区神经元内IP3及IP3R-1表达的变化

    Institute of Scientific and Technical Information of China (English)

    周祎; 刘娟; 黄大可; 桂丽; 汪渊; 贾雪梅

    2011-01-01

    Objective To observe the change of IP3 and IP3R-1's expression in neurons of mice's hippocampal CA1 and CA3 , which had been treated by Apolipoprotein E gene knock-out( ApoE KO ) and high-fat diet. Methods 30 C57BL/6J mice were divided int0 3 groups: the control group ( C group ), ApoE KO group ( KO group ),ApoE KO high-fat diet group ( KO-HF group ). After mice model established, weight and plasma lipid of these mice were measured. The brain tissues of the mice were observed by HE staining, immunohistochemistry staining,and computer image analysis. Results The weight, total cholesterol, triglyceride . low-density lipoprotein cholesterol of the KO and KO-HF groups were all higher than those in the control group( P < 0. 05 ). The H-E staining showed that, in the KO and KO-HF groups, the pyramidal cell layers ranged sparse and cell body were relatively small. Compared with C group, The average optical density of IP3 and IP3R-1 in neurons of hippocampal CA1 and CA3 in the KO group reduced. The average optical density in the KO-HF group reduced obviously( P < 0. 05 ).Conclusion ApoE KO and high-fat diet can decrease the expression level of IP3 and IP3 R-1 in neurons of hippocampal CA1 and CA3. These two proteins ( IP3 and IP3R-1 ) might take participate in the pathologic process in Alzheimer disease which caused by the abnormal ApoE.%目的 观察载脂蛋白E(ApoE)基因敲除(KO)及高脂饮食小鼠海马CA1和CA3区神经元内三磷酸肌醇(IP3)和三磷酸肌醇受体-Ⅰ(IP3R-1)表达的变化.方法 将30只C57BL/6J小鼠分为对照组(C组)、ApoE KO组(KO组)、ApoE KO高脂饮食组(KO-HF组).小鼠造模成功后称重;取血检测血脂;取小鼠脑组织分别进行HE染色、免疫组织化学染色和计算机图像分析.结果 与C组比较,KO、KO-HF组体重、总胆固醇、甘油三酯及低密度脂蛋白胆固醇含量明显升高(P<0.05).HE染色观察到,KO和KO-HF组小鼠海马锥体细胞排列较

  15. Novel Node Structures for IP over WDM Networks

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    IP over WDM is being envisioned as one of the most attractive architectures for the next generation Internet. This paper introduces two novel backbone node structures for IP over WDM networks. These node structures can be used as improvement approaches to DPDP (default path and dedicated path) method. The flow classifiers, which are the main components of these node structures, and the performance metrics of these structures, are also discussed.

  16. IPS Classic. Science, art and nature: a case report.

    Science.gov (United States)

    Culp, L; Roach, R R

    1995-01-01

    The ceramo-metal restoration still forms the backbone of modern restorative dentistry, despite many new systems. This article discusses a unique ceramo-metal system, its advantages, and clinical and technical applications; teamwork between the dentist and the technician is emphasized. IPS Classic (Ivoclar Williams) is a ceramic system with several exclusive features. It encompasses Color Visions, a computer-generated shade system, and the IPS Impulse modifier system allows the ceramist unlimited creativity in color development.

  17. Towards Multi-Stage Intrusion Detection using IP Flow Records

    OpenAIRE

    Muhammad Fahad Umer; Muhammad Sher; Imran Khan

    2016-01-01

    Traditional network-based intrusion detection sys-tems using deep packet inspection are not feasible for modern high-speed networks due to slow processing and inability to read encrypted packet content. As an alternative to packet-based intrusion detection, researchers have focused on flow-based intrusion detection techniques. Flow-based intrusion detection systems analyze IP flow records for attack detection. IP flow records contain summarized traffic information. However, flow data is very ...

  18. Identifying IP Blocks with Spamming Bots by Spatial Distribution

    Science.gov (United States)

    Yun, Sangki; Kim, Byungseung; Bahk, Saewoong; Kim, Hyogon

    In this letter, we develop a behavioral metric with which spamming botnets can be quickly identified with respect to their residing IP blocks. Our method aims at line-speed operation without deep inspection, so only TCP/IP header fields of the passing packets are examined. However, the proposed metric yields a high-quality receiver operating characteristics (ROC), with high detection rates and low false positive rates.

  19. Survivable IP/MPLS-Over-WSON Multilayer Network Optimization

    OpenAIRE

    Ruiz M.; Pedrola O.; Velasco L.; Careglio D.; Fernandez-Palacios J.; Junyent G.

    2011-01-01

    Network operators are facing the problem of dimensioning their networks for the expected huge IP traffic volumes while keeping constant or even reducing the connectivity prices. Therefore, new architectural solutions able to cope with the expected traffic increase in a more cost-effective way are needed. In this work, we study the survivable IP/multi-protocol label switching (MPLS) over wavelength switched optical network (WSON) multilayer network problem as a capital expenditure (CAPEX...

  20. miR-10b、miR-18b及miR-130b在肺癌中的表达研究%Expression of miR-10b、miR-18b and miR-130b in lung cancer

    Institute of Scientific and Technical Information of China (English)

    曾龙剑; 吴锡南; 杨凯云; 李苹; 徐俪烜; 姚树祥; 何越峰

    2014-01-01

    目的 探讨miR-10b、miR-18b及miR-130b在肺癌中的表达特征及其在肺癌发生发展过程中的作用.方法 收集47例肺癌住院患者的癌组织和癌旁组织,利用Trizol提取总RNA,实时荧光定量PCR检测基因相对表达量.结果 肺癌组织与癌旁组织中miR-10b(t=2.83,P<0.05)、miR-18b(t=2.88,P<0.05)及miR-130b(t =2.46,P<0.05)表达量均差异存在显著性.比较鳞癌、腺癌和癌旁组织中miR-10b(F =7.16,P<0.05)、miR-18b(F =4.11,P<0.05)及miR-130b(F =3.43,P<0.05)的表达量也有显著性.癌组织中miR-10b与pri-miR-10b表达量存在负相关关系(r=-0.50,P<0.05) . 癌组织与临床指标之间的关系中miR-18b、miR-130b与KI67呈正相关(P<0.05),pri-miR-10b与TOPOⅡ、pri-miR-130b与MRP呈负相关(P<0.05).结论 miR-10b、miR-18b及miR-130b在癌组织中表达量上调,可能具有促进肺癌发生发展的作用.

  1. [Application for Lifestyle disease by iPS cells technologies].

    Science.gov (United States)

    Takashima, Yasuhiro

    2016-03-01

    Currently it is less advanced to understand the pathology of lifestyle disease by using iPS cells because there is partly less direct connection between life style disease and iPS cells. So much more scientists focus on regenerative medicine such as beta cells therapy using iPS cells technologies. It will be indeed a powerful tool to generate beta cells from iPS cells as even in type2 diabetes patients, hyposecretion of insulin from beta cells in pancreas is one of causes. Another reason is complexity of the pathology of life style disease. There are a lot of reasons to cause lifestyle disease. Lifestyle diseases include cancer, chronic liver disease, Type 2 diabetes, heart disease, metabolic syndrome, chronic renal failure, stroke, and obesity. Since obesity is one of major causes of lifestyle diseases, we want to focus on adipogenesis from iPS cells in this review. We analysed and established the differentiation protocol into adipocytes from mouse ES cells and human iPS cells. The other point in this review is the starting pluripotent cells for differentiation. Quality of pluripotent stem cells are one of most critical factors to succeed in getting well-differentiated cells. Recently, we have developed new naive human pluripotent stem cells (PSC),"Reset cells". Naive PSC have more similar to human epibast cells than conventional human PSC. They will be more ideal cells for differentiation because of their hypomethylated status and earlier stage of development.

  2. CATCHprofiles: clustering and alignment tool for ChIP profiles.

    Directory of Open Access Journals (Sweden)

    Fiona G G Nielsen

    Full Text Available Chromatin Immuno Precipitation (ChIP profiling detects in vivo protein-DNA binding, and has revealed a large combinatorial complexity in the binding of chromatin associated proteins and their post-translational modifications. To fully explore the spatial and combinatorial patterns in ChIP-profiling data and detect potentially meaningful patterns, the areas of enrichment must be aligned and clustered, which is an algorithmically and computationally challenging task. We have developed CATCHprofiles, a novel tool for exhaustive pattern detection in ChIP profiling data. CATCHprofiles is built upon a computationally efficient implementation for the exhaustive alignment and hierarchical clustering of ChIP profiling data. The tool features a graphical interface for examination and browsing of the clustering results. CATCHprofiles requires no prior knowledge about functional sites, detects known binding patterns "ab initio", and enables the detection of new patterns from ChIP data at a high resolution, exemplified by the detection of asymmetric histone and histone modification patterns around H2A.Z-enriched sites. CATCHprofiles' capability for exhaustive analysis combined with its ease-of-use makes it an invaluable tool for explorative research based on ChIP profiling data. CATCHprofiles and the CATCH algorithm run on all platforms and is available for free through the CATCH website: http://catch.cmbi.ru.nl/. User support is available by subscribing to the mailing list catch-users@bioinformatics.org.

  3. CATCHprofiles: Clustering and Alignment Tool for ChIP Profiles

    DEFF Research Database (Denmark)

    G. G. Nielsen, Fiona; Galschiøt Markus, Kasper; Møllegaard Friborg, Rune

    2012-01-01

    Chromatin Immuno Precipitation (ChIP) profiling detects in vivo protein-DNA binding, and has revealed a large combinatorial complexity in the binding of chromatin associated proteins and their post-translational modifications. To fully explore the spatial and combinatorial patterns in ChIP-profil......Chromatin Immuno Precipitation (ChIP) profiling detects in vivo protein-DNA binding, and has revealed a large combinatorial complexity in the binding of chromatin associated proteins and their post-translational modifications. To fully explore the spatial and combinatorial patterns in Ch......IP-profiling data and detect potentially meaningful patterns, the areas of enrichment must be aligned and clustered, which is an algorithmically and computationally challenging task. We have developed CATCHprofiles, a novel tool for exhaustive pattern detection in ChIP profiling data. CATCHprofiles is built upon...... a computationally efficient implementation for the exhaustive alignment and hierarchical clustering of ChIP profiling data. The tool features a graphical interface for examination and browsing of the clustering results. CATCHprofiles requires no prior knowledge about functional sites, detects known binding patterns...

  4. Inositol hexakisphosphate (IP6) generated by IP5K mediates cullin-COP9 signalosome interactions and CRL function

    National Research Council Canada - National Science Library

    Paul C. Scherer; Yan Ding; Zhiqing Liu; Jing Xu; Haibin Mao; James C. Barrow; Ning Wei; Ning Zheng; Solomon H. Snyder; Feng Rao

    2016-01-01

    .... We now report that inositol hexakisphosphate (IP6) is a major physiologic determinant of the CRL-CSN interface, which includes a hitherto unidentified electrostatic interaction between the N-terminal acidic tail of CSN subunit 2 (CSN2...

  5. ChIPpeakAnno: a Bioconductor package to annotate ChIP-seq and ChIP-chip data

    Directory of Open Access Journals (Sweden)

    Pagès Hervé

    2010-05-01

    Full Text Available Abstract Background Chromatin immunoprecipitation (ChIP followed by high-throughput sequencing (ChIP-seq or ChIP followed by genome tiling array analysis (ChIP-chip have become standard technologies for genome-wide identification of DNA-binding protein target sites. A number of algorithms have been developed in parallel that allow identification of binding sites from ChIP-seq or ChIP-chip datasets and subsequent visualization in the University of California Santa Cruz (UCSC Genome Browser as custom annotation tracks. However, summarizing these tracks can be a daunting task, particularly if there are a large number of binding sites or the binding sites are distributed widely across the genome. Results We have developed ChIPpeakAnno as a Bioconductor package within the statistical programming environment R to facilitate batch annotation of enriched peaks identified from ChIP-seq, ChIP-chip, cap analysis of gene expression (CAGE or any experiments resulting in a large number of enriched genomic regions. The binding sites annotated with ChIPpeakAnno can be viewed easily as a table, a pie chart or plotted in histogram form, i.e., the distribution of distances to the nearest genes for each set of peaks. In addition, we have implemented functionalities for determining the significance of overlap between replicates or binding sites among transcription factors within a complex, and for drawing Venn diagrams to visualize the extent of the overlap between replicates. Furthermore, the package includes functionalities to retrieve sequences flanking putative binding sites for PCR amplification, cloning, or motif discovery, and to identify Gene Ontology (GO terms associated with adjacent genes. Conclusions ChIPpeakAnno enables batch annotation of the binding sites identified from ChIP-seq, ChIP-chip, CAGE or any technology that results in a large number of enriched genomic regions within the statistical programming environment R. Allowing users to pass their

  6. PENINGKATAN HASIL BELAJAR IPS MELALUI METODE SIMULASI DENGAN REINFORCEMENT DI SMPN 2 JETIS BANTUL

    Directory of Open Access Journals (Sweden)

    Ratna Wulandari

    2016-12-01

    Full Text Available Penelitian ini bertujuan untuk meningkatkan aktivitas dan hasil belajar IPS di kelas IX C SMPN 2 Jetis Bantul setelah diterapkan metode simulasi dengan reinforcement. Penelitian ini merupakan penelitian tindakan kelas (classrrom action research menggunakan desain Kemmis & Taggart. Subyek penelitian adalah siswa kelas IX C SMPN 2 Jetis Bantul tahun pelajaran 2013/2014. Tindakan yang diterapkan adalah penerapan metode simulasi dengan reinforcement untuk dalam pembelajaran IPS. Teknik pengumpulan data yang digunakan adalah  observasi dan tes. Teknik observasi digunakan untuk mengetahui aktivitas siswa dalam pembelajaran IPS dan tes digunakan untuk menunjukkan hasil belajar siswa. Penelitian ini terlaksana dalam dua siklus. Siklus I mengunakan model simulasi dengan reinforcement verbal dan siklus II menggunakan model simulasi dengan reinforcement non verbal.  Hasil penelitian menunjukkan bahwa penggunaan model simulasi dengan reinforcement meningkatkan aktivitas belajar IPS dari 14,28% pada siklus I menjadi 87,49% pada siklus II, hasil belajar IPS meningkat dari rata-rata kelas 81,07 pada siklus I menjadi 81,25 pada siklus II. Dengan demikian metode simulasi dengan reinforcement dapat meningkatkan hasil belajar IPS siswa kelas IX C SMPN 2 Jetis Bantul.   IMPROVING THE SOCIAL STUDIES LEARNING RESULT THROUGH SIMULATION METHODS WITH REINFORCEMENT AT SMPN 2 JETIS BANTUL Abstract This research aims to improve the learning activity and social studies learning result at year IX C of  SMPN 2 Jetis Bantul  after appliying Simulation Methods with reinforcement. This research was a classrrom action research study using Kemmis & Taggart design. The subjects in this research were year IX C students SMPN 2 Jetis Bantul in the academic year of 2013/2014. Treatment that applied is simulation methods with reinforcement to improve the activity and social studies learning result. The data were collected through observation and tests. Observation technique was

  7. dPeak: high resolution identification of transcription factor binding sites from PET and SET ChIP-Seq data.

    Directory of Open Access Journals (Sweden)

    Dongjun Chung

    Full Text Available Chromatin immunoprecipitation followed by high throughput sequencing (ChIP-Seq has been successfully used for genome-wide profiling of transcription factor binding sites, histone modifications, and nucleosome occupancy in many model organisms and humans. Because the compact genomes of prokaryotes harbor many binding sites separated by only few base pairs, applications of ChIP-Seq in this domain have not reached their full potential. Applications in prokaryotic genomes are further hampered by the fact that well studied data analysis methods for ChIP-Seq do not result in a resolution required for deciphering the locations of nearby binding events. We generated single-end tag (SET and paired-end tag (PET ChIP-Seq data for σ⁷⁰ factor in Escherichia coli (E. coli. Direct comparison of these datasets revealed that although PET assay enables higher resolution identification of binding events, standard ChIP-Seq analysis methods are not equipped to utilize PET-specific features of the data. To address this problem, we developed dPeak as a high resolution binding site identification (deconvolution algorithm. dPeak implements a probabilistic model that accurately describes ChIP-Seq data generation process for both the SET and PET assays. For SET data, dPeak outperforms or performs comparably to the state-of-the-art high-resolution ChIP-Seq peak deconvolution algorithms such as PICS, GPS, and GEM. When coupled with PET data, dPeak significantly outperforms SET-based analysis with any of the current state-of-the-art methods. Experimental validations of a subset of dPeak predictions from σ⁷⁰ PET ChIP-Seq data indicate that dPeak can estimate locations of binding events with as high as 2 to 21 bp resolution. Applications of dPeak to σ⁷⁰ ChIP-Seq data in E. coli under aerobic and anaerobic conditions reveal closely located promoters that are differentially occupied and further illustrate the importance of high resolution analysis of ChIP-Seq data.

  8. Intelligent Advanced Communications IP Telephony Feasibility for the U.S. Navy. Volume 1

    Science.gov (United States)

    2007-10-01

    the vocal tract and the vibration of the vocal chord . If the model of the vocal tract and its vibrations is good, then the error between the actual...from Department of Computer Science at the Columbia University, University of Maryland Advanced Computer Studies, Cisco Systems Inc., and Vocal ...for several open source VoIP software packages, with a special focus of VOCAL , Vovida’s own open communication application library. While VOCAL is the

  9. 时域激电数据的Cole - Cole模型参数反演与应用%Cole-Cole Model Inversion Based on IP Data in Time-Domain

    Institute of Scientific and Technical Information of China (English)

    蓝常斌; 罗润林; 王兆龙; 罗天涯; 黄正杰; 周文; 董超; 邓文志

    2012-01-01

    The spectrum parameters of Cole -Cole model can be used to distinguish the polarization bodies and explore deep ore deposit by induced polarization abnormalities. Cole - Cole Model parameters can be mainly in-versed by spectral induced polarization (S[P) data. While SIP data is observed by many frequencies, the working efficiency in field is very low. However,the time-domain induced polarization(TDIP) method is applied widely. In order to abstract Cole - Cole model parameters from TDIP,a least square method is presented to inverse Cole -Cole medel parameters based on TDIP data in this paper. Because of the interconversion between SIP and TDIP, when different time-lapse apparent chargeabilities are enough, Cole - Cole medel parameters could be calculated with TDIP data. This method is simple and the inverted result is reliable. The practice indicates that Cole - Cole Model parameters can enrich the information of TDIP method and distinguish some abnormalities which apparent resistivity and apparent chargbilities could not. So this method could improve the exploration ability of TDIP.%Cole - Cole模型的频谱参数可以用于区分引起激电异常的极化体和寻找深部矿(化)体.Cole - Cole模型参数以往由频谱激电数据求得,但它需要观测多个数据,野外工作效率低,而时间域数据的使用却是十分普遍.据此,提出了一种基于时间域激电数据的Cole - Cole模型参数的最小二乘反演方法.该方法根据频谱激电和时间域激电可以相互转换的理论,在时间域激电中,用几个不同延时段的视充电率计算出Cole - Cole模型参数.实例表明:利用Cole - Cole模型参数极大地丰富了时间域激发极化法的有效信息,可以区分一些常规激电法所不能区分的异常体,提高了时间域激电勘探能力.

  10. Voice Quality Estimation in Combined Radio-VoIP Networks for Dispatching Systems

    Directory of Open Access Journals (Sweden)

    Jiri Vodrazka

    2016-01-01

    Full Text Available The voice quality modelling assessment and planning field is deeply and widely theoretically and practically mastered for common voice communication systems, especially for the public fixed and mobile telephone networks including Next Generation Networks (NGN - internet protocol based networks. This article seeks to contribute voice quality modelling assessment and planning for dispatching communication systems based on Internet Protocol (IP and private radio networks. The network plan, correction in E-model calculation and default values for the model are presented and discussed.

  11. Design and Implementation of a VoIP Broadcasting Service over Embedded Systems in a Heterogeneous Network Environment

    Directory of Open Access Journals (Sweden)

    Jenq-Shiou Leu

    2014-01-01

    Full Text Available As the digitization is integrated into daily life, media including video and audio are heavily transferred over the Internet nowadays. Voice-over-Internet Protocol (VoIP, the most popular and mature technology, becomes the focus attracting many researches and investments. However, most of the existing studies focused on a one-to-one communication model in a homogeneous network, instead of one-to-many broadcasting model among diverse embedded devices in a heterogeneous network. In this paper, we present the implementation of a VoIP broadcasting service on the open source—Linphone—in a heterogeneous network environment, including WiFi, 3G, and LAN networks. The proposed system featuring VoIP broadcasting over heterogeneous networks can be integrated with heterogeneous agile devices, such as embedded devices or mobile phones. VoIP broadcasting over heterogeneous networks can be integrated into modern smartphones or other embedded devices; thus when users run in a traditional AM/FM signal unreachable area, they still can receive the broadcast voice through the IP network. Also, comprehensive evaluations are conducted to verify the effectiveness of the proposed implementation.

  12. LegumeIP 2.0--a platform for the study of gene function and genome evolution in legumes.

    Science.gov (United States)

    Li, Jun; Dai, Xinbin; Zhuang, Zhaohong; Zhao, Patrick X

    2016-01-04

    The LegumeIP 2.0 database hosts large-scale genomics and transcriptomics data and provides integrative bioinformatics tools for the study of gene function and evolution in legumes. Our recent updates in LegumeIP 2.0 include gene and protein sequences, gene models and annotations, syntenic regions, protein families and phylogenetic trees for six legume species: Medicago truncatula, Glycine max (soybean), Lotus japonicus, Phaseolus vulgaris (common bean), Cicer arietinum (chickpea) and Cajanus cajan (pigeon pea) and two outgroup reference species: Arabidopsis thaliana and Poplar trichocarpa. Moreover, the LegumeIP 2.0 features the following new data resources and bioinformatics tools: (i) an integrative gene expression atlas for four model legumes that include 550 array hybridizations from M. truncatula, 962 gene expression profiles of G. max, 276 array hybridizations from L. japonicas and 56 RNA-Seq-based gene expression profiles for C. arietinum. These datasets were manually curated and hierarchically organized based on Experimental Ontology and Plant Ontology so that users can browse, search, and retrieve data for their selected experiments. (ii) New functions/analytical tools to query, mine and visualize large-scale gene sequences, annotations and transcriptome profiles. Users may select a subset of expression experiments and visualize and compare expression profiles for multiple genes. The LegumeIP 2.0 database is freely available to the public at http://plantgrn.noble.org/LegumeIP/.

  13. Design and Implementation of a VoIP Broadcasting Service over Embedded Systems in a Heterogeneous Network Environment

    Science.gov (United States)

    Lin, Wei-Hsiang; Hsieh, Wen-Bin; Lo, Chien-Chih

    2014-01-01

    As the digitization is integrated into daily life, media including video and audio are heavily transferred over the Internet nowadays. Voice-over-Internet Protocol (VoIP), the most popular and mature technology, becomes the focus attracting many researches and investments. However, most of the existing studies focused on a one-to-one communication model in a homogeneous network, instead of one-to-many broadcasting model among diverse embedded devices in a heterogeneous network. In this paper, we present the implementation of a VoIP broadcasting service on the open source—Linphone—in a heterogeneous network environment, including WiFi, 3G, and LAN networks. The proposed system featuring VoIP broadcasting over heterogeneous networks can be integrated with heterogeneous agile devices, such as embedded devices or mobile phones. VoIP broadcasting over heterogeneous networks can be integrated into modern smartphones or other embedded devices; thus when users run in a traditional AM/FM signal unreachable area, they still can receive the broadcast voice through the IP network. Also, comprehensive evaluations are conducted to verify the effectiveness of the proposed implementation. PMID:25300280

  14. Design and implementation of a VoIP broadcasting service over embedded systems in a heterogeneous network environment.

    Science.gov (United States)

    Leu, Jenq-Shiou; Lin, Wei-Hsiang; Hsieh, Wen-Bin; Lo, Chien-Chih

    2014-01-01

    As the digitization is integrated into daily life, media including video and audio are heavily transferred over the Internet nowadays. Voice-over-Internet Protocol (VoIP), the most popular and mature technology, becomes the focus attracting many researches and investments. However, most of the existing studies focused on a one-to-one communication model in a homogeneous network, instead of one-to-many broadcasting model among diverse embedded devices in a heterogeneous network. In this paper, we present the implementation of a VoIP broadcasting service on the open source-Linphone-in a heterogeneous network environment, including WiFi, 3G, and LAN networks. The proposed system featuring VoIP broadcasting over heterogeneous networks can be integrated with heterogeneous agile devices, such as embedded devices or mobile phones. VoIP broadcasting over heterogeneous networks can be integrated into modern smartphones or other embedded devices; thus when users run in a traditional AM/FM signal unreachable area, they still can receive the broadcast voice through the IP network. Also, comprehensive evaluations are conducted to verify the effectiveness of the proposed implementation.

  15. Induced polarization (IP) imaging for the improved characterization of clay-rich landslides

    Science.gov (United States)

    Gallistl, Jakob; Flores-Orozco, Adrián; Ottowitz, David; Gautier, Stéphanie; Malet, Jean-Philippe

    2017-04-01

    Landslides pose a significant socio-economical natural hazard, in particular those developed in clay-rich environments due to their suddenness, volumes and propagations. Typically induced by meteorological phenomena (i.e. strong precipitations) the understanding of water circulation in clayey landslides is critical for an adequate hydromechanical modeling and the development of accurate early warning systems. In recent years, electrical resistivity tomography (ERT) has been widely applied to characterize the structure of landslides and monitoring of hydrogeological changes, aiming at an improved understanding of the water flow patterns. However, clay-rich sediments limit the applicability of ERT due the low contrast in the electrical signatures of clay minerals and saturated soil. Taking into account the strong induced polarization (IP) response in the presence of clay minerals, we propose the application of the IP imaging method to improve the delineation of structures and heterogeneities controlling water flow circulation in clayey landslides. To better evaluate the IP method at different geological conditions, here we discuss IP imaging results for data collected at two different landslides located in France (La Valette in the South East Alps, and Lodève located in the southern border of the Massif Central Massif) as well as two sites in Austria. These sites were selected due to the extensive non-geophysical information available and the ERT monitoring data measured over several years. IP measurements have been collected in both time- and frequency-domain to further assess the advantages of the different techniques in particular towards the quantification of hydrogeological parameters, such as dominating grain size and hydraulic conductivity. Imaging results demonstrate an improved lithological characterization, permitting the delineation of the sliding plane as well as a better discrimination of clay lenses with enhanced resolution. Nevertheless the clay

  16. Proposal of Secure VoIP System Using Attribute Certificate

    Science.gov (United States)

    Kim, Jin-Mook; Jeong, Young-Ae; Hong, Seong-Sik

    VoIP is a service that changes the analogue audio signal into a digital signal and then transfers the audio information to the users after configuring it as a packet; and it has an advantage of lower price than the existing voice call service and better extensibility. However, VoIP service has a system structure that, compared to the existing PSTN (Public Switched Telephone Network), has poor call quality and is vulnerable in the security aspect. To make up these problems, TLS service was introduced to enhance the security. In practical system, however, since QoS problem occurs, it is necessary to develop the VoIP security system that can satisfy QoS at the same time in the security aspect. In this paper, a user authentication VoIP system that can provide a service according to the security and the user through providing a differential service according to the approach of the users by adding AA server at the step of configuring the existing VoIP session is suggested. It was found that the proposed system of this study provides a quicker QoS than the TLS-added system at a similar level of security. Also, it is able to provide a variety of additional services by the different users.

  17. An effective dynamic reconfiguration algorithm for IP over WDM networks

    Science.gov (United States)

    Yu, Hongfang; Zhou, Tao; Wang, Sheng; Li, Lemin

    2005-02-01

    WDM (Wavelength Division Multiplexing) technology can provide multiple wavelengths on a fiber. IP directly over WDM (or IP over WDM) has become the hot topic of industry. A promising approach for building an IP over WDM network is that a logical network consisting of the wavelength channels (lightpaths) is built on the physical WDM network. Then, IP traffic is carried on the logical topology, by utilizing the MPLS (Multiple Protocol Label Switching) or GMPLS (Generalized MPLS). When the traffic demand pattern changes in the IP layer, the network performance may become poor. In order to improve the network performance, the virtual topology can be reconfigured to suit the changing traffic patterns. In this paper, dynamic slowly-adaptation scheme (e.g. tearing down a lightpath that is lightly loaded or setting up a new lightpath when congestion occurs) is adopted. How to select the source and the destination nodes of the new lightpath to be added and the underutilized lightpath to be deleted if it is necessary to do so is our key issue. Four selection ways are developed. These ways are evaluated through detail simulations and various performances are investigated.

  18. Immunopotentiator from Pantoea agglomerans 1 (IP-PA1) Promotes Murine Hair Growth and Human Dermal Papilla Cell Gene Expression.

    Science.gov (United States)

    Wakame, Koji; Okawa, Hiroshi; Komatsu, Ken-Ich; Nakata, Akifumi; Sato, Keisuke; Ingawa, Hiroyuki; Kohchi, Chie; Nishizawa, Takashi; Soma, Gen-Ichiro

    2016-07-01

    The lipopolysaccharide (LPS)-like compound derived from Pantoea agglomerans (immunopotentiator from Pantoea agglomerans 1 (IP-PA1)) has been used not only as dietary supplement or cosmetic for humans, but also by Japanese veterinarians as an anti-tumor, anti-allergy, "keep a fine coat of fur" and hair growth-promoting functional food for dogs and cats. In the present study, we focused on the hair growth-promoting effects of IP-PA1 on a hair-shaved animal model and its mechanism of action. We also investigated its potential on gene expression after stimulating human dermal papilla cells with IP-PA1. The hair on the back of a C3H/HeN mouse was shaved and IP-PA1 was orally administered or applied to the skin. The status of hair growth was observed and recorded for 14 days. Skin was collected and histological tissue examination was performed with respect to hair growth status using hematoxylin and eosin staining. After IP-PA1 administration (2 and 10 μg/ml) to human dermal papilla cell culture system for 24 h, fibroblast growth factor-7 (FGF-7) and vascular endothelial growth factor (VEGF) mRNA expression were measured using real-time polymerase chain reaction (PCR) analysis. IP-PA1, when given orally, showed a tendency to promote hair growth in mice. In addition, skin application also significantly promoted hair growth, while histopathological examinations further demonstrated hair elongation from dermal papilla cells. In the human dermal papilla cell culture system, significant FGF-7 and VEGF mRNA expressions were observed (p<0.05). An underlying mechanism of gene expression by which IP-PA1 promotes hair growth was suggested to be different from that of medicine and traditional hair tonics, such as minoxidil and adenosine. Copyright© 2016 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.

  19. Generation of male germ cells from induced pluripotent stem cells (iPS cells): an in vitro and in vivo study.

    Science.gov (United States)

    Zhu, Yong; Hu, Hong-Liang; Li, Peng; Yang, Shi; Zhang, Wei; Ding, Hui; Tian, Ru-Hui; Ning, Ye; Zhang, Ling-Ling; Guo, Xi-Zhi; Shi, Zhan-Ping; Li, Zheng; He, Zuping

    2012-07-01

    Recent studies have reported that induced pluripotent stem (iPS) cells from mice and humans can differentiate into primordial germ cells. However, whether iPS cells are capable of producing male germ cells is not known. The objective of this study was to investigate the differentiation potential of mouse iPS cells into spermatogonial stem cells and late-stage male germ cells. We used an approach that combines in vitro differentiation and in vivo transplantation. Embryoid bodies (EBs) were obtained from iPS cells using leukaemia inhibitor factor (LIF)-free medium. Quantitative PCR revealed a decrease in Oct4 expression and an increase in Stra8 and Vasa mRNA in the EBs derived from iPS cells. iPS cell-derived EBs were induced by retinoic acid to differentiate into spermatogonial stem cells (SSCs), as evidenced by their expression of VASA, as well as CDH1 and GFRα1, which are markers of SSCs. Furthermore, these germ cells derived from iPS cells were transplanted into recipient testes of mice that had been pre-treated with busulfan. Notably, iPS cell-derived SSCs were able to differentiate into male germ cells ranging from spermatogonia to round spermatids, as shown by VASA and SCP3 expression. This study demonstrates that iPS cells have the potential to differentiate into late-stage male germ cells. The derivation of male germ cells from iPS cells has potential applications in the treatment of male infertility and provides a model for uncovering the molecular mechanisms underlying male germ cell development.

  20. Chromatin immunoprecipitation and multiplex sequencing (ChIP-Seq) to identify global transcription factor binding sites in the nematode Caenorhabditis elegans.

    Science.gov (United States)

    Brdlik, Cathleen M; Niu, Wei; Snyder, Michael

    2014-01-01

    The global identification of transcription factor (TF) binding sites is a critical step in the elucidation of the functional elements of the genome. Several methods have been developed that map TF binding in human cells, yeast, and other model organisms. These methods make use of chromatin immunoprecipitation, or ChIP, and take advantage of the fact that formaldehyde fixation of living cells can be used to cross-link DNA sequences to the TFs that bind them in vivo. In ChIP, the cross-linked TF-DNA complexes are sheared by sonication, size fractionated, and incubated with antibody specific to the TF of interest to generate a library of TF-bound DNA sequences. ChIP-chip was the first technology developed to globally identify TF-bound DNA sequences and involves subsequent hybridization of the ChIP DNA to oligonucleotide microarrays. However, ChIP-chip proved to be costly, labor-intensive, and limited by the fixed number of probes available on the microarray chip. ChIP-Seq combines ChIP with massively parallel high-throughput sequencing (see Explanatory Chapter: Next Generation Sequencing) and has demonstrated vast improvement over ChIP-chip with respect to time and cost, signal-to-noise ratio, and resolution. In particular, multiplex sequencing can be used to achieve a higher throughput in ChIP-Seq analyses involving organisms with genomes of lower complexity than that of human (Lefrançois et al., 2009) and thereby reduce the cost and amount of time needed for each result. The multiplex ChIP-Seq method described in this section has been developed for Caenorhabditis elegans, but is easily adaptable for other organisms.

  1. Examination of a Viral Infection Mimetic Model in Human iPS Cell-Derived Insulin-Producing Cells and the Anti-Apoptotic Effect of GLP-1 Analogue.

    Directory of Open Access Journals (Sweden)

    Megu Yamaguchi Baden

    Full Text Available Viral infection is associated with pancreatic beta cell destruction in fulminant type 1 diabetes mellitus. The aim of this study was to investigate the acceleration and protective mechanisms of beta cell destruction by establishing a model of viral infection in pancreatic beta cells.Polyinosinic:polycytidylic acid was transfected into MIN6 cells and insulin-producing cells differentiated from human induced pluripotent stem cells via small molecule applications. Gene expression was analyzed by real-time PCR, and apoptosis was evaluated by caspase-3 activity and TUNEL staining. The anti-apoptotic effect of Exendin-4 was also evaluated.Polyinosinic:polycytidylic acid transfection led to elevated expression of the genes encoding IFNα, IFNβ, CXCL10, Fas, viral receptors, and IFN-inducible antiviral effectors in MIN6 cells. Exendin-4 treatment suppressed the elevated gene expression levels and reduced polyinosinic:polycytidylic acid-induced apoptosis both in MIN6 cells and in insulin-producing cells from human induced pluripotent stem cells. Glucagon-like peptide-1 receptor, protein kinase A, and phosphatidylinositol-3 kinase inhibitors counteracted the anti-apoptotic effect of Exendin-4.Polyinosinic:polycytidylic acid transfection can mimic viral infection, and Exendin-4 exerted an anti-apoptotic effect both in MIN6 and insulin-producing cells from human induced pluripotent stem cells.

  2. Research on Performance between IP and Vector Forwarding

    Directory of Open Access Journals (Sweden)

    Aqun Zhao

    2013-03-01

    Full Text Available With the rapid development of network size and great increase of Internet traffic, it has become a crucial work to improve the forwarding performance of the core devices of Internet or the routers. In this study, the analysis and comparison of instruction cycles occupied by the machine in the process of route lookup in IP forwarding and vector forwarding were carried out which may determine the forwarding performance of a router. The simulation experiments were also made to study the forwarding efficiency of IP forwarding and vector forwarding. The theoretical and experimental results prove that the vector forwarding method is more efficient than IP forwarding which can provide powerful evidence to the application of vector network in the Internet.

  3. Mobile location services over the next generation IP core network

    DEFF Research Database (Denmark)

    Thongthammachart, Saowanee; Olesen, Henning

    2003-01-01

    network is changing from circuit-switched to packet-switched technology and evolving to an IP core network based on IPv6. The IP core network will allow all IP devices to be connected seamlessly. Due to the movement detection mechanism of Mobile IPv6, mobile terminals will periodically update......Mobile communication networks are evolving towards smaller cells, higher throughput, better security and provision of better services. Wireless short-range technologies, such as the WLAN 802.11 standards family and Bluetooth, are expected to play a major role in future networks. The mobile core...... their current point of attachment to the network and hence provide the current location of the mobile user automatically. The convergence of wireless short-range networks, mobile networks and Internet technology will provide the mobile user's location without any add-in equipment for location measurement...

  4. Firewall for Dynamic IP Address in Mobile IPv6

    Science.gov (United States)

    Qiu, Ying; Bao, Feng; Zhou, Jianying

    Mobile communication is becoming the mainstream with the rapid growth of mobile devices penetrating our daily life. More and more mobile devices such as mobile phones, personal digital assistants, notebooks etc, are capable of Internet access. Mobile devices frequently change their communication IP addresses in mobile IPv6 network following its current attached domain. This raises a big challenge for building firewall for mobile devices. The conventional firewalls are primarily based on IPv4 networks where the security criteria are specified only to the fixed IP addresses or subnets, which apparently do not apply to mobile IPv6. In this paper we propose three solutions for mobile IPv6 firewall. Our approaches make the firewall adaptive to dynamic IP addresses in mobile IPv6 network. They have different expense and weight corresponding to different degree of universality. The paper focuses the study more from practical aspect.

  5. Congestion Avoidance in IP Based CDMA Radio Access Network

    Directory of Open Access Journals (Sweden)

    Syed Shakeel Hashmi

    2011-02-01

    Full Text Available CDMA is an important air interface technologies for cellular wireless networks. As CDMAbasedcellular networks mature, the current point-to-point links will evolve to an IP-based Radio AccessNetwork (RAN. mechanisms must be designed to control the IP Radio Access Network congestion.This Paper implements a congestion control mechanism using Router control and channelcontrol method for IP-RAN on CDMA cellular network. The Router control mechanism uses the featuresof CDMA networks using active Queue Management technique to reduce delay and to minimize thecorrelated losses. The Random Early Detection Active Queue Management scheme (REDAQM is to berealized for the router control for data transmission over the radio network using routers as the channel.The channel control mechanism control the congestion by bifurcating the access channel into multiplelayer namely RACH, BCCH and DCH for data accessing. The proposed paper work is realized usingMatlab platform.

  6. Capturing value from Intellectual Property (IP) in a global environment

    DEFF Research Database (Denmark)

    Beukel, Karin; Cassiman, Bruno

    2017-01-01

    Globalization should provide firms with an opportunity to leverage their know-how and reputation across countries to create value. However, it remains challenging for them to actually capture that value using traditional Intellectual Property (IP) tools. In this paper, we document the strong growth...... are causing backlogs and delays in numerous Patent and Trademarks Offices and litigation over IP rights is expensive, with an uncertain outcome. Moreover, local governments can succeed in transferring value to local firms and influencing global market positions by using IP laws and other regulations...... discuss in our concluding section. Global companies will need to organize cross-functional value capture teams focused on appropriating value from their know-how and reputation by combining different institutional, market, and nonmarket tools, depending on the institutional and business environment...

  7. Load characterization and anomaly detection for voice over IP traffic.

    Science.gov (United States)

    Mandjes, Michel; Saniee, Iraj; Stolyar, Alexander L

    2005-09-01

    We consider the problem of traffic anomaly detection in IP networks. Traffic anomalies typically arise when there is focused overload or when a network element fails and it is desired to infer these purely from the measured traffic. We derive new general formulae for the variance of the cumulative traffic over a fixed time interval and show how the derived analytical expression simplifies for the case of voice over IP traffic, the focus of this paper. To detect load anomalies, we show it is sufficient to consider cumulative traffic over relatively long intervals such as 5 min. We also propose simple anomaly detection tests including detection of over/underload. This approach substantially extends the current practice in IP network management where only the first-order statistics and fixed thresholds are used to identify abnormal behavior. We conclude with the application of the scheme to field data from an operational network.

  8. Generation and application of human iPS cells

    Institute of Scientific and Technical Information of China (English)

    CUI Ghun; RAO LingJun; CHENG LinZhao; XIAO Lei

    2009-01-01

    Human embryonic stem (ES) cells are capable of unlimited proliferation and maintenance of pluripo-tency in vitro; these properties may lead to potential applications in regenerative medicine.However,immune rejection hampers the allogenic application of human ES cells.Over-expression of several specific transcription factors has been used to reprogram human adult cells into induced pluripotent stem (iPS) cells,which are similar to hESCs in many aspects.The iPS technique makes it possible to produce patient-specific pluripotent stem cells for transplantation therapy without immune rejection.However,some challenges remain,including viral vector integration into the genome,the existence of exogenous oncogenic factors,and low induction efficiency.Here,we review recent advances in human iPS methodology,as well as remaining challenges and its potential applications.

  9. Integrated Differentiated Survivability in IP over WDM Networks

    Institute of Scientific and Technical Information of China (English)

    Wei Wei; Qing-Ji Zeng

    2004-01-01

    The problem of differentiated Multi-Layer Integrated Survivability (MLIS) in IP over WDM networks is studied, which is decomposed into three sub-problems: survivable strategies design (SSD), spare capacity dimensioning (SCD), and dynamic survivable routing (DSR). A related work of network survivability in IP over WDM networks is firstly provided, and adaptive survivable strategies are also designed. A new Integrated Shared Pool (ISP) approach for SCD is then proposed, which is formulated by using integer-programming theory. Moreover, a novel survivable routing scheme called Differentiated Integrated Survivability Algorithm (DISA) for DSR is developed. Simulation results show that the proposed integrated survivability scheme performs much better than other solutions (e.g., "highest layer recovery" and "lowest layer recovery" schemes) in terms of traffic blocking ratio, spare resource requirement, and average traffic recovery ratio in IP over WDM networks.

  10. Capturing value from IP in a global environment

    DEFF Research Database (Denmark)

    Alcacer, Juan; Beukel, Karin; Cassiman, Bruno

    of patenting globally. For trademarks and industrial designs, globalization has created more potential infringers and an increase in piracy, as evidenced by a significant increase in customs seizures. The problems with IP even go beyond individual firms, as when governments use IP policies to favor local firms...... section. Global companies will need to organize cross-functional value capture teams focused on appropriating value from their know-how and reputation by combining different institutional, market, and non-market tools, depending on the institutional and business environment in a particular region.......This paper documents the strong growth in tools used by firms to protect their intellectual property (IP), develop their know-how, and build and maintain their reputation globally. We focus on three tools that have become increasingly important in the last several decades: patents, trademarks...

  11. A Novel Framework for IP DiffServ over Optical Burst Switching Networks

    Institute of Scientific and Technical Information of China (English)

    Ke-Ping Long; Yun Li; Rodney S.Tucker; Chong-Gang Wang

    2004-01-01

    This paper presents a novel framework for IP Differentiated Services (DiffServ) over optical burst switching (OBS), namely, DS-OBS. The network architecture, functional model of edge nodes and core nodes, the control packet format, a novel burst assembly scheme at ingress nodes and scheduling algorithm of core nodes are presented. The basic idea is to apply DiffServ capable burst assembly at ingress nodes and perform different per hop behavior (PHB) electronic treatments for control packets of different QoS class services at core nodes. Simulation results show that the proposed schemes can provide the best differentiated service for expedited forwarding (EF), assured forwarding (AF) and best effort (BE) services in terms of end-to-end deay, throughput and IP packet loss probability.

  12. Aplicación del modelo polinomial en la evaluación de la uniformidad de riego del aspersor autocompensado Nelson R10

    Directory of Open Access Journals (Sweden)

    Fanny del Rocío Rodríguez-Jarama

    2015-01-01

    Full Text Available Los métodos de evaluación de la uniformidad de riego estiman las condiciones hidráulicas de funcionamiento del sistema de riego y su influencia en la aplicación de láminas de agua, con base en las necesidades hídricas de un cultivo. El modelo de evaluación utilizado en la presente investigación fue el polinomial. El ensayo evaluó el funcionamiento del sistema de riego con 4 presiones de operación distintas, medidas a la salida de la válvula del módulo de riego, y por cada presión de operación se tomaron 16 datos de caudales, en 5 módulos de riego instalado con el aspersor autocompensado Nelson R10. Como resultado se obtuvo el coeficiente de uniformidad, el coeficiente de distribución, el área adecuadamente irrigada y el factor de adecuación del riego. Uno de los principales resultados fue la no existencia de diferencia significativa entre las presiones mientras esta se mantengan en el rango de autocompensación; por otro lado, en el coeficiente de distribución sí existen diferencias significativas entre los tratamientos, a pesar de que todos dan valores mayores al 90%, en contraste con los porcentajes del área relativa adecuadamente regada, que no superan el 50% en ninguno de los tratamientos. Con lo anterior se pudo validar y verificar que el aspersor Nelson R10 autocompensado, una vez instalado en campo, cumple con las características técnicas de operación expuestas en el catálogo del fabricante, sin embargo, sí se debe manejar y controlar la lámina de riego aplicada.

  13. Predicting Persistent Back Symptoms by Psychosocial Risk Factors: Validity Criteria for the OMPSQ and the HKF-R 10 in Germany.

    Directory of Open Access Journals (Sweden)

    E Riewe

    Full Text Available 10% of all individuals in Germany develop persistent symptoms due to nonspecific back pain (NSBP causing up to 90% of direct and indirect expenses for health care systems. Evidence indicates a strong relationship between chronic nonspecific back pain and psychosocial risk factors. The Örebro Musculoskeletal Pain Screening Questionnaire (ÖMPSQ and the German Heidelberger Kurzfragebogen Rückenschmerz (HKF-R 10 are deemed valid in prediction of persistent pain, functional loss or amount of sick leave. This study provides and discusses validity criteria for these questionnaires using ROC-curve analyses. Quality measurements included sensitivity and specificity, likelihood-ratio related test-efficiencies and clinical utility in regard to predictive values.265 patients recruited from primary and secondary care units completed both questionnaires during the same timeframe. From the total, 133 patients returned a 6-month follow-up questionnaire to assess the validity criteria for outcomes of pain, function and sick leave.Based on heterogeneous cut-offs for the ÖMPSQ, sensitivity and specificity were moderate for outcome of pain (72%/75%. Very high sensitivity was observed for function (97%/57% and high specificity for sick leave (63%/85%. The latter also applied to the HKF-R 10 (pain 50%/84%. Proportions between sensitivity and specificity were unbalanced except for the ÖMPSQ outcome of pain. Likelihood-ratios and positive predictive values ranged from low to moderate.Although the ÖMPSQ may be considered useful in identification of long-term functional loss or pain, over- and underestimation of patients at risk of chronic noncspecific back pain led to limited test-efficiencies and clinical utility for both questionnaires. Further studies are required to quantify the predictive validity of both questionnaires in Germany.

  14. Optimizing Low Speed VoIP Network for Rural Next Generation Network (R-NGN

    Directory of Open Access Journals (Sweden)

    Yoanes Bandung

    2007-11-01

    Full Text Available In this research, we propose an optimization method based-on E-Model for designing an efficient low speed VoIP network for Rural Next Generation Network (R-NGN. We are choosing 128 kbps and 256 kbps bandwidth as the typical community link to be used in the designing of R-NGN infrastructure. The method is based on selection of some VoIP network parameters such as voice coder, communication protocol, packet loss level, network utilization and resource allocation. We draw analytic approach for achieving rating value (R of E-model that represent level of quality of service. In this approach, we focus on delay and packet loss calculation to find the rating value. We state the rating value = 70 as minimum level of quality of service for each call, equivalent to 3.6 of Mean Opinion Score (MOS. In our experiments, either G.723.1 5.3 kbps or G.729 is chosen for maximizing the number of VoIP calls, it depends on link utilization and level of packet loss.

  15. AN EFFICIENT PACKET SCHEDULING ALGORITHM FOR 4G IP-BASED MOBILE NETWORKS

    Directory of Open Access Journals (Sweden)

    Hussaim Mohammed

    2010-09-01

    Full Text Available Next generation mobile networks are expected to provide seamless personal mobile communication and quality of service (QoS. Lossless handoff is a key issue for providing the QoS. This paper presents 4G node B Architecture, a two-layer downlink queuing model and proposes a scheduling mechanism for providing lossless handoff and QoS in mobile networks, which exploit IP as a transport technology for transferring datagrams between base stations and the high-speed downlink packet access (HSDPA at the radio layer. In order to reduce handoff packet dropping rate at the radio layer and packet forwarding rate at the IP layer and to provide high system performance, new scheduling algorithms are performed at both IP and radio layer, which exploit handoff priority scheduling principles and take into account buffer occupancy and channel conditions. Performance results obtained by computer simulation show that, by exploiting the downlink queuing model and scheduling algorithms, the system is able to provide low handoff packet dropping rate, low packet forwarding rate, and high downlink throughput.

  16. [The genetic safety of induced pluripotent stem (iPS) cells].

    Science.gov (United States)

    Chen, Qian; Shi, Qing-Hua

    2012-03-01

    Since Takahashi and Yamanaka first generated induced pluripotent stem (iPS) cells from mouse fibroblasts successfully in 2006, iPS cells have rapidly become a new hotspot in the field of stem cells research because of their broad potential application prospects. Meanwhile, more and more attentions are paid to the genetic safety of iPS cells. This article summarizes recent findings on genetic safety of iPS cells and reviews the possible causes leading to genetic instability of iPS cells. Hopefully, this review is helpful to improve the induction of iPS cells and obtain genetically safe iPS cells.

  17. The Verb Always Leaves IP in V2 Clauses

    DEFF Research Database (Denmark)

    Schwartz, Bonnie D.; Vikner, Sten

    2007-01-01

    The verb second (V2) phenomenon, as it is found in the Germanic languages, has been the focus of much attention within recent syntactic research. In most of the literature on V2, it is assumed that the verb in all V2 clauses has moved to a head position outside IP, e.g. Cº. In Schwartz & Vikner...... analysis in which the verb moves into an Xº which is the sister of IP may be compatible with what we say here. Various alternatives to this analysis have been explored in the literature, and here we will address two in particular: One alternative is that there is an asymmetry between subject...

  18. VoIP Steganography and Its Detection - A Survey

    CERN Document Server

    Mazurczyk, Wojciech

    2012-01-01

    Steganography is an ancient art that encompasses various techniques of information hiding, the aim of which is to secret information into a carrier message. Steganographic methods are usually aimed at hiding the very existence of the communication. Due to the rise in popularity of IP telephony, together with the large volume of data and variety of protocols involved, it is currently attracting the attention of the research community as a perfect carrier for steganographic purposes. This paper is a survey of the existing VoIP steganography (steganophony) methods and their countermeasures.

  19. IP Lookup as a Critical Functionality of Packet Processors

    Directory of Open Access Journals (Sweden)

    Z. G. Čiča

    2013-06-01

    Full Text Available Packet processing represents the most significant part of a router’s data plane and has a large impact on router’s scalability. Packet processing consists of many functions and some of them can become critical for the future router’s scalability. In this paper, the IP lookup as one of the most critical packet processing functions is analyzed to determine its impact on the Internet router’s scalability. Also, in this paper we propose a new modification of our previously proposed IP lookup algorithm BPFL.

  20. Voice over IP for Sony Ericsson Cellular Phones

    OpenAIRE

    Theander, Petter; Hultgren, Thomas

    2005-01-01

    This report presents an investigation of the possibilities to implement voice over IP (VoIP) in Sony Ericsson cellular phones. The results from this investigation show that it is partially possible to implement such a solution. The best option for doing so is to make use of the support for the Session Initiation Protocol and the Real-time Transport Protocol offered by the architecture. Another goal is to evaluate if Bluetooth is able to handle the requirements needed for the solution. The who...

  1. USB Dual-Mode Function IP Core Development

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    This paper describes the specification and implement of a Universal Serial Bus (USB) dual-mode function IP core used for embedded system. Controlled by micro controller/CPU, the novel IP core can function as a USB host controller or USB peripheral controller. When configured as a USB host controller, it supports all USB 1.1 transaction types; supports automatic preamble insertion, and automatic SOF generation and transmission. Otherwise, when it is configured as a USB device by a microprocessor, it operates as a USB peripheral controller compliant with USB2.0 specification.

  2. Interfacing the Controllogics PLC over Ethernet/IP.

    Energy Technology Data Exchange (ETDEWEB)

    Kasemir, K. U. (Kay-Uwe); Dalesio, L. R. (Leo R.)

    2001-01-01

    The Allen-Bradley ControlLogix [1] line of programmable logic controllers (PLCs) offers several interfaces: Ethernet, ControlNet, DeviceNet, RS-232 and others. The ControlLogix Ethernet interface module 1756-ENET uses EtherNet/IP, the ControlNet protocol [2], encapsulated in Ethernet packages, with specific service codes [3]. A driver for the Experimental Physics and Industrial Control System (EPICS) has been developed that utilizes this EtherNet/IP protocol for controllers running the vxWorks RTOS as well as a Win32 and Unix/Linux test program. Features, performance and limitations of this interface are presented.

  3. 浅析IP-SAN和FC-SAN解决方案

    Institute of Scientific and Technical Information of China (English)

    林文栋

    2010-01-01

    存储技术发展日新月异,SAN可说是DAS网络化发展趋势下的产物.本文简介绍IP SAN和FC SAN,对IP SAN和FC SAN的架构以及构建成本、可扩展性、易用性、兼容性、稳定性和速度等的比较,认为各有千秋,两者之间应取长补短,互相共存.

  4. Research of differentiated QoS routing in GMPLS-based IP/WDM networks

    Science.gov (United States)

    Wang, YiYun; Zeng, QingJi; Cao, JunWen

    2004-04-01

    At this point in technology's evolution, the simplicity, elegance, extensibility, and broad compatibility of the Internet protocol suite has made it the automatic choice for most forms of communication. The attempts at resolution of this apparent dichotomy consist of a collection of technologies and philosophies known as Quality of Service. In an IP network, QoS defines the ability to compensate for traffic characteristics without compromising average throughput. Clearly, optimizing QoS performance for all traffic types on an IP network presents a daunting challenge. To partially address this challenge, several Internet Engineering Task Force groups have been working on standardized approaches for IP-based QoS technologies. The IETF"s approaches fall into four categories: prioritization using differentiated services, reservation using integrated services, label switching using multi-protocol label switching, bandwidth management using the subnet bandwidth manager. Differentiated services classify per-hop behaviors on the basis of a Diffserv code point attached to the type of service byte in each packet"s IP header. This DSCP approach represents a form of soft QoS that rather coarsely classifies services through packet marking. The differentiated QoS routing in GMPLS-based IP/WDM Networks are a promising candidate for the next generation optical Internet networks. By using a unified control plane, such networks make more efficient usage of network resources both at the IP layer and the WDM optical layer. In this paper, we consider prioritized routing of bandwidth-guaranteed Label Switched paths (LSPs) providing service differentiation between classes of high and normal priority traffic. The QoS delay requirements are assumed to be translated into bandwidth and O-E-O conversion requirements. We present a graphical representation of the integrated network state which is different from other conventional graphical representations in that it models the cost of usage of

  5. Internet Connection of a Broadband Wireless IP Network Based on a Fast Mobile IP Algorithm with Link Layer Support

    Institute of Scientific and Technical Information of China (English)

    XIONGYan; MIAOFuyou; HUABei; WANGXingfu

    2003-01-01

    Commercialized wireless LANs (local area networks) are emerging one after another. Because a lot of useful information and resources are residing in the wired network, Internet, the strong requirement to access In-ternet for mobile hosts in wireless LANs is growing more and more. In this paper, we simply describe the topoi-ogy and feature of a broadband wireless Internet protocol network (BWIPN) in which data transmitted on wireless link is encapsulated within Internet protocol (IP) packets.To connect to Internet, there is an Internet connecting server (ICS) which is also a router within every BWIPN.A fast mobile IP hand-offalgorithm with link layer supportto reduce handoff overhead has been proposed to providemobility support for wireless terminals (WTs, i.e. note-book computers) roaming among BWIPNs without chang-ing their IP addresses. An indirect TCP (transfer control protocol) scheme is used to improve the performance ofwireless TCP. To match data transmission rate on the wire-less link with that on the wired link, an adaptive wireless congestion control algorithms are added at IP layers in the WT and the ICS. Both performance analysis and experi-mental result have shown that the fast mobile IP hand-off algorithm reduces the hand-off delay by about 10 times and TCP disconnection times is reduced greatly, compared with the three known mobile IP hand-off algorithms. By our work, users on WTs can explore Internet using Web browser, File Transfer Protocol, Telnet and Ping utilities while moving among different BWIPNs very well.

  6. G0/G1 arrest and S phase inhibition of human cancer cell lines by inositol hexaphosphate (IP6).

    Science.gov (United States)

    El-Sherbiny, Y M; Cox, M C; Ismail, Z A; Shamsuddin, A M; Vucenik, I

    2001-01-01

    Inositol hexaphosphate (InsP6 or IP6) has shown a striking anti-cancer activity in both in vivo and in vitro models. In an attempt to elucidate the mechanism(s) underlying the anti-neoplastic potential of IP6, we investigated its effect on cell cycle progression of MCF-7 estrogen receptor (ER)-positive and MDA-MB 231 ER-negative human breast cancer cell lines and HT-29 human colon cancer cells. The anti-proliferative effect of IP6 was evaluated using dual-parameter flow cytometric measurements of DNA content, versus the incorporation of 5-bromo-2-deoxyuridine (BrdU) to determine cells actively synthesizing DNA. Combined analysis of the expression of cell cycle-related proteins, proliferation marker Ki-67 and proliferating cell nuclear antigen (PCNA) versus DNA content were used to determine the amount of proliferating cells in each phase, engaged in cell cycle transit. After 3 days of treatment with 5 mM IP6, S-phase, as estimated by BrdU uptake, was significantly decreased in all three cell lines (p = 0.002). MCF-7 and HT-29 cells accumulated in the G0/G1 range of DNA contents (p = 0.002 and p = 0.001, respectively). MDA MB-231 cells transiently accumulated in G0/G1 only after 2 days (p = 0.01). There was a significant decrease in the percentage of Ki-67 expression in IP6-treated cells, from 82.8+/-3.0% to 66.8+/-4.2% in MCF-7 (p = 0.007), from 93.4+/-4.6% to 71.7+/-3.3% in MDA-MB 231 (p = 0.004), and from 95.2+/-1.2% to 73.5+/-2.5% in HT-29 cells (p = 0.002) respectively. PCNA expression levels were also significantly decreased by IP6 in all three cell lines (MCF-7 p = 0.0007; MDA-MB 231 p = 0.0006; HT-29 p = 0.0001). These results show that IP6 controls the progression of cells through the cycle by decreasing S- phase and arresting cells in the G0/G1-phase of the cell cycle. A significant decrease in the expression of proliferation markers indicated that IP6 disengaged cells from actively cycling. Further investigations of cell cycle regulators may lead us to a

  7. Inositol hexaphosphate (IP6) enhances the anti-proliferative effects of adriamycin and tamoxifen in breast cancer.

    Science.gov (United States)

    Tantivejkul, Kwanchanit; Vucenik, Ivana; Eiseman, Julie; Shamsuddin, AbulKalam M

    2003-06-01

    The current treatment of breast carcinomas recognizes the importance of combination therapy in order to increase efficacy and decrease side effects of conventional chemotherapy. Inositol hexaphosphate (IP6), a naturally occurring polyphosphorylated carbohydrate, has shown a significant anti-cancer effect in various in vivo and in vitro models, including breast cancer. In this study, we investigated the in vitro growth inhibitory activity of IP6 in combination with adriamycin or tamoxifen, against three human breast cancer cell lines: estrogen receptor (ER) alpha-positive MCF-7, ER alpha-negative MDA-MB 231 and adriamycin-resistant MCF-7 (MCF-7/Adr) using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Much lower concentrations of IP6 were required after 96 h of treatment to inhibit the growth of MCF-7/Adr cells than MCF-7 cells; the IC50 for MCF-7/Adr cells was 1.26 mM compared to 4.18 mM for MCF-7 cells. The ER-negative MDA-MB 231 cells were also highly sensitive to IP6 with IC50 being 1.32 mM. To determine the effects of IP6 in combination with either adriamycin or tamoxifen, the median effect principle and Webb's fraction method were used to determine the combination index (CI) and the statistical differences. Growth suppression was markedly increased when IP6 was administered prior to the addition of adriamycin, especially against MCF-7 cells (CI = 0.175 and p IP6 was administered after tamoxifen in all three cell lines studied (CI = 0.343, 0.701 and 0.819; p IP6 with LC50 values ranging from 0.91 to 5.75 mM (n = 10). Our data not only confirm that IP6 alone inhibits the growth of breast cancer cells; but it also acts synergistically with adriamycin or tamoxifen, being particularly effective against ER alpha-negative cells and adriamycin-resistant cell lines.

  8. Modeling and Detection of Camouflaging Worm using IP Traceback

    Directory of Open Access Journals (Sweden)

    S Preetha

    2012-04-01

    Full Text Available Active worms pose major security threats to the Internet. This is due to the ability of active worms to propagate in an automated fashion as they continuously compromise computers on the Internet. Active worms evolve during their propagation, and thus, pose great challenges to defend against them. A new class of active worms, referred to as Camouflaging Worm (C-Worm in short. The C-Worm is different from traditional worms because of its ability to intelligently manipulate its scan traffic volume over time. Thereby, the C-Worm camouflages its propagation from existing worm detection systems based on analyzing the propagation traffic generated by worms. The characteristics of the C-Worm and conduct a comprehensive comparison between its traffic and non-worm traffic (background traffic. The two types of traffic are barely distinguishable in the time domain. However, their distinction is clear in the frequency domain, due to the recurring manipulative nature of the C-Worm. Motivated by observations, designed a novel spectrum-based scheme to detect the C-Worm. The Power Spectral Density (PSD distribution of the scan traffic volume and its corresponding Spectral Flatness Measure (SFM to distinguish the C-Worm traffic from background traffic. Using a comprehensive set of detection metrics and real-world traces as background traffic, the extensive performance evaluations on proposed spectrum-based detection scheme. The performance data clearly demonstrates that our scheme can effectively detect the C-Worm propagation. Furthermore, show the generality of spectrum-based scheme in effectively detecting not only y the C-Worm, but traditional worms as well

  9. A New Luminous Outburst from SN 2009ip

    Science.gov (United States)

    Drake, A. J.; Howerton, S.; McNaught, R.; Djorgovski, S. G.; Mahabal, A. A.; Graham, M. J.; Williams, R.; Prieto, J.; Catelan, M.; Christensen, E.; Larson, S.

    2012-08-01

    Further to Drake et al. (2010, Atel#2897), we report the discovery of a new bright outburst from spectroscopically confirmed LBV (Supernova Impostor) SN 2009ip (Maza et al. 2009; CBET#1928, Berger et al. 2009, ATEL#2184; Smith et al. 2010; Foley et al. 2011) in images taken by the Siding Spring Survey (rmn/>SSS).

  10. An Overview of the IP Enforcement System in China

    Institute of Scientific and Technical Information of China (English)

    Wu Yuhe

    2005-01-01

    @@ For more than two decades after the Trademark Law of the People's Republic of China entered into force in 1983,China has formulated a series of IP-related laws and regulations, including among other things, the Patent Law, Trademark Law, Copyright Law and Unfair Competition Law. Besides, it has acceded to many international IP treaties and established an adequate system for IP protection. In particular, China has drawn more on the mature practices of the Western countries with respect to the conditions and requirements for grant of the intellectual property rights, which has made the legal system for the protection of the intellectual property rights in China readily understandable and acceptable to foreign interested parties. By contrast, China has mostly followed the existing civil, administrative and criminal liability system in its construction of the IP-related enforcement system, thus rendering it more "characteristic of China". In fact, this "unique"system provides interested parties with more opportunities and approaches for them to enforce their rights.

  11. IPS Space Weather Research: Korea-Japan-UCSD

    Science.gov (United States)

    2015-04-27

    systems. c) The potential for using heliospheric Faraday rotation for measuring Bz remotely. To make headway on these items in the allotted time...workshop where this information will be presented. To make headway on the analysis of IPS g-level data as a proxy for heliospheric density, we wish

  12. PERCEPTIVE APPROACH FOR ROUTE OPTIMIZATION IN MOBILE IP

    Directory of Open Access Journals (Sweden)

    Vinay Kumar Nigam

    2010-12-01

    Full Text Available The recent advances in wireless communication technology and the unprecedented growth of the Internet have paved the way for wireless networking and IP mobility. Mobile Internet protocol[1,2] has been designed within the IETF to support the mobility[2] of users who wish to connect to the internet and maintain communications as they move from place to place. Mobile IPV6 allows a mobile node to talk directly to its peers while retaining the ability to move around and change the currently used IP addresses. This mode of operation is called Route Optimization[7,10].In this approach, the correspondent node learns a binding between the Mobile nodes permanent home address and its current temporary care-of-address. This introduces several security vulnerabilities to Mobile IP, among them the most important one is the authentication and authorization of binding updates. This paper describes the Route optimization by the introduction of mobility. In this paper , we proposed a new efficient technique for route optimization in mobile IP for smoothly communication while MN moving from one network domain to other without losing the connection. Our technique will also be improve the path in intra-network communication[9].

  13. Crosslayer Survivability in Overlay-IP-WDM Networks

    Science.gov (United States)

    Pacharintanakul, Peera

    2010-01-01

    As the Internet moves towards a three-layer architecture consisting of overlay networks on top of the IP network layer on top of WDM-based physical networks, incorporating the interaction between and among network layers is crucial for efficient and effective implementation of survivability. This dissertation has four major foci as follows:…

  14. IP-Based TV Technologies, Services and Multidisciplinary Applications

    NARCIS (Netherlands)

    Bonastre, O.M.; Montpetit, M.J.; Cesar Garcia, P.S.

    2013-01-01

    The move to Internet Protocol (IP)-based content delivery services has challenged the television industry by allowing high-quality television content to be delivered using the Internet, wired and wireless, private and public. The new convergence paradigm is already playing out its disruptive role as

  15. Performance Comparison and Analysis of IP and MPLS Networks

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    With the increasing need for Quality of Service(QoS),the traditional IP network is becoming less competitive. In order to give readers quantitative and convictive performance comparisons between IP and MPLS network,we choose Berkeleys NS-2 simulator[1] in our research on network throughput and end-to-end TCP behavior. Using NS-2 simulator,we analyze the performance of a network with MPLS Traffic Engineering(MPLS-TE).Specifically,the throughput and TCP end-to-end delay time under two different queuing algorithms are obtained and compared between the MPLS network and traditional IP network. We find that in the IP network using SFQ queuing algorithm will help traffic balance compared to the simple FIFO scheme,but it still can not utilize the available bandwidth,which in turn leads to the poor performance as in the FIFO case.With MPLS-TE, the throughput will be higher and the end-to-end TCP delay time remains almost the same even in heavy load case.We notice that if flows are isolated partially,using SFQ scheme will have a comparable performance as the fully isolation case using FIFO scheme in a MPLS network,and that if flows are isolated fully for FIFO and SFQ schemes,both will have the same result.Finally,we conclude that a better queuing algorithm will help reduce the label space requirement.

  16. Performance Comparison and Analysis of IP and MPLS Networks

    Institute of Scientific and Technical Information of China (English)

    LIUEr-wu; CAOMing-cui; 等

    2001-01-01

    With the increasing need for Quality of Service(QoS), the traditional IP network is becoming less com-petitive.In order to give readers quantitative and convictive performance comparisons between IP and MPLS net-work, we choose Berkeley's NS-2 simulator[1] in our research on network throughput and end-to-end TCP behavior.Using NS-2 simulator,we analyze the performance of a network with MPLS Traffic Engineering (MPLS-TE).Specifically, the throughput and TCP end-to-end delay time under two different queuing algorithms are obtained and compared between the MPLS network and traditional IP network .We find that in the IP network using SFQ queuing algorithm will help traffic balance compared to the simple FIFO scheme,but it still can ont utilize the available bandwidth,which in turn leads to the poor performance as in the FIFO case.With MPLS-TE,the throughput will be higher and the end-to-end TCP delay time remains almost the same even in heavy load case.We notice that if flows are isolated partially,using SFQ scheme will have a comparable performance as the fully isola-tion case using FIFO scheme in a MPLS network, and that if flows are isolated fully for FIFO and SFQ schemes, both will have the same result.Finally,we conclude that a better queuing algorithm will help reduce the label space requirement.

  17. Capturing value from IP in a global environment

    DEFF Research Database (Denmark)

    Alcacer, Juan; Beukel, Karin; Cassiman, Bruno

    will need to combine different institutional, market and non-market mechanisms. The precise combination of tools will depend on the local and regional institutional and market conditions. Abstract: This paper documents the strong growth in tools used by firms to protect their intellectual property (IP...

  18. VoIP makes voice heard--functionality is up.

    Science.gov (United States)

    Wade, Phil; Wakelam, Mathew

    2008-05-01

    Phil Wade, sales and marketing director, and Mathew Wakelam, VoIP product manager, at alarm and communication specialist Static Systems Group, discuss wireless VoLP's potential in healthcare establishments and examine how, in particular, the technology can be successfully integrated with nurse call systems.

  19. Managing wireless IP-Connectivity Experience as Mobile Social Media

    NARCIS (Netherlands)

    Rigolin Ferreira Lopes, R.; van Beijnum, Bernhard J.F.; dos Santos Moreira, Edson

    2010-01-01

    Social media is the meaningful digital content that results from interaction or collaboration between users through web-based applications, e.g. blogs and virtual communities. While on the move, people can create or consume mobile social media using portable devices with IP-connectivity. Our

  20. IP over optical multicasting for large-scale video delivery

    Science.gov (United States)

    Jin, Yaohui; Hu, Weisheng; Sun, Weiqiang; Guo, Wei

    2007-11-01

    In the IPTV systems, multicasting will play a crucial role in the delivery of high-quality video services, which can significantly improve bandwidth efficiency. However, the scalability and the signal quality of current IPTV can barely compete with the existing broadcast digital TV systems since it is difficult to implement large-scale multicasting with end-to-end guaranteed quality of service (QoS) in packet-switched IP network. China 3TNet project aimed to build a high performance broadband trial network to support large-scale concurrent streaming media and interactive multimedia services. The innovative idea of 3TNet is that an automatic switched optical networks (ASON) with the capability of dynamic point-to-multipoint (P2MP) connections replaces the conventional IP multicasting network in the transport core, while the edge remains an IP multicasting network. In this paper, we will introduce the network architecture and discuss challenges in such IP over Optical multicasting for video delivery.

  1. Load characterization and anomaly detection for voice over IP traffic

    NARCIS (Netherlands)

    M.R.H. Mandjes (Michel); I. Saniee; A. Stolyar

    2005-01-01

    textabstractWe consider the problem of traffic anomaly detection in IP networks. Traffic anomalies typically arise when there is focused overload or when a network element fails and it is desired to infer these purely from the measured traffic. We derive new general formulae for the variance of the

  2. Voice over IP - Eine Einführung

    OpenAIRE

    Fey, Marcus

    2006-01-01

    Eine kurze Einführung zu "Voice over IP" (dem Telefonieren über Datennetze). Es wird ein Überblick über technische Anforderungen und Lösungen geben. Behandelte Gebiete sind Audio-Codecs, das Transportprotokoll RTP sowie die Signalisierungsdienste SIP und H.323.

  3. Pine Engraver, Ips pini, in the Western United States (FIDL)

    Science.gov (United States)

    Sandra J. Kegley; R. Ladd Livingston; Kenneth E. Gibson

    1997-01-01

    The pine engraver, Ips pini (Say), is one of the most common and widely distributed bark beetles in North America. It occurs from southern Appalachia north to Maine and Quebec, westward across the northern United States and Canada, into the interior of Alaska, throughout the Pacific Coast States and the Rocky Mountain region, to northern Mexico. In the western United...

  4. Multicast and IP Multicast Support in Wireless Sensor Networks

    Directory of Open Access Journals (Sweden)

    Jorge Sá Silva

    2008-03-01

    Full Text Available Multicast potentially optimises bandwidth consumption and node resources, when several users simultaneously participate in a communication session. Nevertheless, contrary to the expectations, IP multicast has not experienced widespread deployment, with the exception of IPTV. On the other hand, emerging Wireless Sensor Network (WSN applications could greatly benefit from multicast and constitute another field where multicast can be an effective and efficient technique. The questions are: do multicast advantages hold in WSN scenarios? Can we use IP Multicast functionality in WSNs? This paper discusses and evaluates the use of multicast in WSNs. Specifically, we evaluate the use of Source-Specific Multicast, as it is one of the most promising paradigms for IP networking, considering both IPv4 and IPv6 in WSNs. A sensor platform with IP and multicast support that is being developed in our lab is presented. Concurrently, simulation studies were performed in order to assess the usefulness of multicast in WSNs. The results clearly point to the benefits of the use of this technique in processing and energy-restricted environments such as this one.

  5. Extending Tactical Fleet Communications Through VoIP

    Science.gov (United States)

    2014-09-01

    configurations and led to the following list of configuration recommendations: change to the least used wireless channel in your operating area; find a space...31 a. ADNS QoS ...............................................................................31 b. ADNS UDP and...VoIP QoS .....................................................31 c. ADNS QoS between LANs ......................................................32 d

  6. Strategies for analyzing highly enriched IP-chip datasets

    Directory of Open Access Journals (Sweden)

    Tavaré Simon

    2009-09-01

    Full Text Available Abstract Background Chromatin immunoprecipitation on tiling arrays (ChIP-chip has been employed to examine features such as protein binding and histone modifications on a genome-wide scale in a variety of cell types. Array data from the latter studies typically have a high proportion of enriched probes whose signals vary considerably (due to heterogeneity in the cell population, and this makes their normalization and downstream analysis difficult. Results Here we present strategies for analyzing such experiments, focusing our discussion on the analysis of Bromodeoxyruridine (BrdU immunoprecipitation on tiling array (BrdU-IP-chip datasets. BrdU-IP-chip experiments map large, recently replicated genomic regions and have similar characteristics to histone modification/location data. To prepare such data for downstream analysis we employ a dynamic programming algorithm that identifies a set of putative unenriched probes, which we use for both within-array and between-array normalization. We also introduce a second dynamic programming algorithm that incorporates a priori knowledge to identify and quantify positive signals in these datasets. Conclusion Highly enriched IP-chip datasets are often difficult to analyze with traditional array normalization and analysis strategies. Here we present and test a set of analytical tools for their normalization and quantification that allows for accurate identification and analysis of enriched regions.

  7. Crosslayer Survivability in Overlay-IP-WDM Networks

    Science.gov (United States)

    Pacharintanakul, Peera

    2010-01-01

    As the Internet moves towards a three-layer architecture consisting of overlay networks on top of the IP network layer on top of WDM-based physical networks, incorporating the interaction between and among network layers is crucial for efficient and effective implementation of survivability. This dissertation has four major foci as follows:…

  8. Construction of a nuclear data server using TCP/IP

    Energy Technology Data Exchange (ETDEWEB)

    Kawano, Toshihiko; Sakai, Osamu [Kyushu Univ., Fukuoka (Japan)

    1997-03-01

    We construct a nuclear data server which provides data in the evaluated nuclear data library through the network by means of TCP/IP. The client is not necessarily a user but a computer program. Two examples with a prototype server program are demonstrated, the first is data transfer from the server to a user, and the second is to a computer program. (author)

  9. Displaying digital video over ethernet TCP-IP

    Science.gov (United States)

    Foster, John; Jackson, Paul

    1994-04-01

    Transmission and playback of digital audio and video via TCP/IP has been successfully completed with the use of typical low-end UNIX workstations. In addition, attempts have been made to increase the frame rate by sending only sections of a frame that varied the most.

  10. VLBI data transmission system using multiple IP streams

    Science.gov (United States)

    Uose, Hisao

    We have developed an IP-based data transmission system which can directly replace K4 (ID1) VLBI data recorder. It employs multiple TCP data steams and individual buffers to cope with variable usable bandwidth we encouter when we use shared academic networks. This paper describes the outline of the system and preliminary testing results.

  11. Voice over IP phone calls from your smartphone

    CERN Multimedia

    2014-01-01

    All CERN users do have a Lync account (see here) and can use Instant Messaging, presence and other features. In addition, if your number is activated on Lync IP Phone(1) system then you can make standard phone calls from your computer (Windows/Mac).   Recently, we upgraded the infrastructure to Lync 2013. One of the major features is the possibility to make Voice over IP phone calls from a smartphone using your CERN standard phone number (not mobile!). Install Lync 2013 on iPhone/iPad, Android or Windows Phone, connect to WiFi network and make phone calls as if you were in your office. There will be no roaming charges because you will be using WiFi to connect to CERN phone system(2). Register here to the presentation on Tuesday 29 April at 11 a.m. in the Technical Training Center and see the most exciting features of Lync 2013.   Looking forward to seeing you! The Lync team (1) How to register on Lync IP Phone system: http://information-technology.web.cern.ch/book/lync-ip-phone-serv...

  12. Generation and In Vitro Expansion of Hepatic Progenitor Cells from Human iPS Cells.

    Science.gov (United States)

    Yanagida, Ayaka; Nakauchi, Hiromitsu; Kamiya, Akihide

    2016-01-01

    Stem cells have the unique properties of self-renewal and multipotency (producing progeny belonging to two or more lineages). Induced pluripotent stem (iPS) cells can be generated from somatic cells by simultaneous expression of pluripotent factors (Oct3/4, Klf4, Sox2, and c-Myc). They share the same properties as embryonic stem (ES) cells and can differentiate into several tissue cells, i.e., neurons, hematopoietic cells, and liver cells. Therefore, iPS cells are suitable candidate cells for regenerative medicine and analyses of disease mechanisms.The liver is the major organ that regulates a multitude of metabolic functions. Hepatocytes are the major cell type populating the liver parenchyma and express several metabolic enzymes that are necessary for liver functions. Although hepatocytes are essential for maintaining homeostasis, it is difficult to alter artificial and transplanted cells because of their multifunctionality, donor shortage, and immunorejection risk. During liver development, hepatic progenitor cells in the fetal liver differentiate into both mature hepatocytes and cholangiocytes. As hepatic progenitor cells have bipotency and high proliferation ability, they could present a potential source for generating transplantable cells or as a liver study model. Here we describe the induction and purification of hepatic progenitor cells derived from human iPS cells. These cells can proliferate for a long term under suitable culture conditions.

  13. Inorganic SnIP-type double helices in main group chemistry.

    Science.gov (United States)

    Baumgartner, Maximilian; Weihrich, Richard; Nilges, Tom

    2017-03-01

    Inspired by the synthesis of the first atomic-scale double helix semiconductor SnIP this study deals with the question if more atomistic, inorganic double helix compounds are accessible. With the aid of quantum chemical calculations we have identified 31 candidates by a homoatomic substitution in MXPn, varying the group 4 M-element from Si to Pb, the group 7 X-element from F to I and replacing the pnictide (Pn) phosphorus by arsenic. The double-helical structure of SnIP has been used as the starting model for all candidates and the electronic structure and vibrational spectra were determined within the Framework of Density Functional Theory (DFT). Varying the outer MX or the inner Pn helix led to the conclusion that iodide and bromide containing MXPn compounds show similar structures to SnIP. Here, the calculations indicate interesting effects for electronic band gap tuning. For the highly polarized fluorides a segregation of the helices to more complex MX substructures is predicted.

  14. HMCan: A method for detecting chromatin modifications in cancer samples using ChIP-seq data

    KAUST Repository

    Ashoor, Haitham

    2013-09-09

    Motivation: Cancer cells are often characterized by epigenetic changes, which include aberrant histone modifications. In particular, local or regional epigenetic silencing is a common mechanism in cancer for silencing expression of tumor suppressor genes. Though several tools have been created to enable detection of histone marks in ChIP-seq data from normal samples, it is unclear whether these tools can be efficiently applied to ChIP-seq data generated from cancer samples. Indeed, cancer genomes are often characterized by frequent copy number alterations: gains and losses of large regions of chromosomal material. Copy number alterations may create a substantial statistical bias in the evaluation of histone mark signal enrichment and result in underdetection of the signal in the regions of loss and overdetection of the signal in the regions of gain. Results: We present HMCan (Histone modifications in cancer), a tool specially designed to analyze histone modification ChIP-seq data produced from cancer genomes. HMCan corrects for the GC-content and copy number bias and then applies Hidden Markov Models to detect the signal from the corrected data. On simulated data, HMCan outperformed several commonly used tools developed to analyze histone modification data produced from genomes without copy number alterations. HMCan also showed superior results on a ChIP-seq dataset generated for the repressive histone mark H3K27me3 in a bladder cancer cell line. HMCan predictions matched well with experimental data (qPCR validated regions) and included, for example, the previously detected H3K27me3 mark in the promoter of the DLEC1 gene, missed by other tools we tested. The Author 2013. Published by Oxford University Press. All rights reserved.

  15. The solar wind at solar maximum: comparisons of EISCAT IPS and in situ observations

    Directory of Open Access Journals (Sweden)

    A. R. Breen

    Full Text Available The solar maximum solar wind is highly structured in latitude, longitude and in time. Coronal measurements show a very high degree of variability, with large variations that are less apparent within in situ spacecraft measurements. Interplanetary scintillation (IPS observations from EISCAT, covering distances from 20 to 100 solar radii (RS, are an ideal source of information on the inner solar wind and can be used, therefore, to cast light on its evolution with distance from the Sun. Earlier comparisons of in situ and IPS measurements under solar minimum conditions showed good large-scale agreement, particularly in the fast wind. In this study we attempt a quantitative comparison of measurements made over solar maximum by EISCAT (20–100 RS and the Wind and Ulysses spacecraft (at 215 RS and 300–1000 RS, respectively. The intervals studied were August–September 1999, May 2000, September 2000 and May 2001, the last-named being the period of the second Ulysses fast latitude scan. Both ballistic and – when possible – MHD/ballistic hybrid models were used to relate the data sets, and we compare the results obtained from these two mapping methods. The results of this study suggest that solar wind velocities measured in situ were less variable than those estimated from IPS measurements closer to the Sun, with the greatest divergence between IPS velocities and in situ measurements occurring in regions where steep longitudinal velocity gradients were seen in situ. We suggest that the interaction between streams of solar wind with different velocities leads to "smoothing" of solar wind velocities between 30–60 RS and 1 AU, and that this process continues at greater distances from the Sun.

    Key words. Interplanetary physics (solar wind plasma; sources of the solar wind; instruments and techniques

  16. Zerone: a ChIP-seq discretizer for multiple replicates with built-in quality control

    Science.gov (United States)

    Cuscó, Pol; Filion, Guillaume J.

    2016-01-01

    Motivation: Chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) is the standard method to investigate chromatin protein composition. As the number of community-available ChIP-seq profiles increases, it becomes more common to use data from different sources, which makes joint analysis challenging. Issues such as lack of reproducibility, heterogeneous quality and conflicts between replicates become evident when comparing datasets, especially when they are produced by different laboratories. Results: Here, we present Zerone, a ChIP-seq discretizer with built-in quality control. Zerone is powered by a Hidden Markov Model with zero-inflated negative multinomial emissions, which allows it to merge several replicates into a single discretized profile. To identify low quality or irreproducible data, we trained a Support Vector Machine and integrated it as part of the discretization process. The result is a classifier reaching 95% accuracy in detecting low quality profiles. We also introduce a graphical representation to compare discretization quality and we show that Zerone achieves outstanding accuracy. Finally, on current hardware, Zerone discretizes a ChIP-seq experiment on mammalian genomes in about 5 min using less than 700 MB of memory. Availability and Implementation: Zerone is available as a command line tool and as an R package. The C source code and R scripts can be downloaded from https://github.com/nanakiksc/zerone. The information to reproduce the benchmark and the figures is stored in a public Docker image that can be downloaded from https://hub.docker.com/r/nanakiksc/zerone/. Contact: guillaume.filion@gmail.com Supplementary information: Supplementary data are available at Bioinformatics online. PMID:27288492

  17. A VoIP Privacy Mechanism and its Application in VoIP Peering for Voice Service Provider Topology and Identity Hiding

    CERN Document Server

    Shen, Charles

    2008-01-01

    Voice Service Providers (VSPs) participating in VoIP peering frequently want to withhold their identity and related privacy-sensitive information from other parties during the VoIP communication. A number of existing documents on VoIP privacy exist, but most of them focus on end user privacy. By summarizing and extending existing work, we present a unified privacy mechanism for both VoIP users and service providers. We also show a case study on how VSPs can use this mechanism for identity and topology hiding in VoIP peering.

  18. The backup and technology research base on IP-SAN%基于IP-SAN的备份及技术研究

    Institute of Scientific and Technical Information of China (English)

    黄世权

    2009-01-01

    IP-SAN利用IP网络为传输介质,基于TCP/IP协议构建的存储区域网络.IP-SAN远程备份基于iSCSI技术.发挥了IP网络和IP-SAN的优势.实现了数据的异地备份,确保了数据的安全性.文章分析了IP-SAN备份的方法.备份策略,并重点分析IP-SAN备份的主要关键技术.

  19. CtIP Mutations Cause Seckel and Jawad Syndromes.

    Directory of Open Access Journals (Sweden)

    Per Qvist

    2011-10-01

    Full Text Available Seckel syndrome is a recessively inherited dwarfism disorder characterized by microcephaly and a unique head profile. Genetically, it constitutes a heterogeneous condition, with several loci mapped (SCKL1-5 but only three disease genes identified: the ATR, CENPJ, and CEP152 genes that control cellular responses to DNA damage. We previously mapped a Seckel syndrome locus to chromosome 18p11.31-q11.2 (SCKL2. Here, we report two mutations in the CtIP (RBBP8 gene within this locus that result in expression of C-terminally truncated forms of CtIP. We propose that these mutations are the molecular cause of the disease observed in the previously described SCKL2 family and in an additional unrelated family diagnosed with a similar form of congenital microcephaly termed Jawad syndrome. While an exonic frameshift mutation was found in the Jawad family, the SCKL2 family carries a splicing mutation that yields a dominant-negative form of CtIP. Further characterization of cell lines derived from the SCKL2 family revealed defective DNA damage induced formation of single-stranded DNA, a critical co-factor for ATR activation. Accordingly, SCKL2 cells present a lowered apoptopic threshold and hypersensitivity to DNA damage. Notably, over-expression of a comparable truncated CtIP variant in non-Seckel cells recapitulates SCKL2 cellular phenotypes in a dose-dependent manner. This work thus identifies CtIP as a disease gene for Seckel and Jawad syndromes and defines a new type of genetic disease mechanism in which a dominant negative mutation yields a recessively inherited disorder.

  20. CtIP Mutations Cause Seckel and Jawad Syndromes.

    Directory of Open Access Journals (Sweden)

    Per Qvist

    2011-10-01

    Full Text Available Seckel syndrome is a recessively inherited dwarfism disorder characterized by microcephaly and a unique head profile. Genetically, it constitutes a heterogeneous condition, with several loci mapped (SCKL1-5 but only three disease genes identified: the ATR, CENPJ, and CEP152 genes that control cellular responses to DNA damage. We previously mapped a Seckel syndrome locus to chromosome 18p11.31-q11.2 (SCKL2. Here, we report two mutations in the CtIP (RBBP8 gene within this locus that result in expression of C-terminally truncated forms of CtIP. We propose that these mutations are the molecular cause of the disease observed in the previously described SCKL2 family and in an additional unrelated family diagnosed with a similar form of congenital microcephaly termed Jawad syndrome. While an exonic frameshift mutation was found in the Jawad family, the SCKL2 family carries a splicing mutation that yields a dominant-negative form of CtIP. Further characterization of cell lines derived from the SCKL2 family revealed defective DNA damage induced formation of single-stranded DNA, a critical co-factor for ATR activation. Accordingly, SCKL2 cells present a lowered apoptopic threshold and hypersensitivity to DNA damage. Notably, over-expression of a comparable truncated CtIP variant in non-Seckel cells recapitulates SCKL2 cellular phenotypes in a dose-dependent manner. This work thus identifies CtIP as a disease gene for Seckel and Jawad syndromes and defines a new type of genetic disease mechanism in which a dominant negative mutation yields a recessively inherited disorder.

  1. Binding specificity of R-10G and TRA-1-60/81, and substrate specificity of keratanase II studied with chemically synthesized oligosaccharides.

    Science.gov (United States)

    Nakao, Hiromi; Nagai, Yuko; Kojima, Aya; Toyoda, Hidenao; Kawasaki, Nobuko; Kawasaki, Toshisuke

    2017-03-14

    Recently, we established a mouse monoclonal antibody specific to hiPS/ hES cells, R-10G, which recognizes a type of keratan sulfate. Keratan sulfates (KS) comprise a family of glycosaminoglycans consisting of the repeating unit of [Gal-GlcNAc(6S)]. However, there is a diversity in the degree of sulfation at Gal and GlcNAc residues, and also in the mode of linkage, Galβ1 - 3GlcNAc (type 1) or Galβ1 - 4GlcNAc (type 2). To gain more insight into the binding specificity of R-10G, we carried out an ELISA test on avidin-coated plates using polyethylene glycol (PEG)3-biotinylated derivatives of a series of N-acetyllactosamine tetrasaccharides (keratan sulfates (KSs)). The results suggested that the minimum epitope structure is Galβ1 - 4GlcNAc(6S)β1 - 3Galβ1 - 4GlcNAc(6S)β1 (type 2- type 2 keratan sulfate). Removal of sulfate from GlcNAc(6S) or addition of sulfate to Gal abolished the binding activity almost completely. We also examined the binding specificity of TRA-1-60/81 in the same assay system. The minimum epitope structure was shown to be Galβ1 - 3GlcNAcβ1 - 3Galβ1 - 4GlcNAcβ1 in agreement with the previous study involving glycan arrays (Natunen et al., Glycobiology, 21, 1125-1130 (2011)). Interestingly, however, TRA-1-60/81 was shown to bind to Galβ1 - 3GlcNAc(6S)β1 - 3Galβ1 - 4GlcNAc(6S)β1 (type 1- type 2 keratan sulfate) dose-dependently, being more than one-third the binding activity toward Galβ1 - 3GlcNAcβ1 - 3Galβ1 - 4GlcNAcβ1 than in the case of TRA-1-60. In addition, a substrate specificity study on keratanase II revealed that keratanase II degraded not only "type 2-type 2 keratan sulfate" but also "type 1-type 2 keratan sulfate", significantly.

  2. Acarbose reduces blood glucose by activating miR-10a-5p and miR-664 in diabetic rats.

    Directory of Open Access Journals (Sweden)

    Qian Zhang

    Full Text Available MicroRNAs (miRNAs are non-coding RNA molecules involved in the post-transcriptional regulation of a large number of genes, including those involved in glucose metabolism. Acarbose is an α-glucosidase inhibitor that improves glycemic control by decreasing the intestinal absorption of glucose, thereby decreasing the elevation of postprandial blood glucose. However, acarbose is poorly absorbed into the blood stream from the gut. Therefore, the exact mechanisms by which acarbose affects glucose metabolism are unclear. This study investigated the effect of acarbose on glucose metabolism in diabetic rats and tested the hypothesis that acarbose acts directly through miRNA-regulated expression in the intestinal epithelium. Rats were divided into four groups: a control group, a diabetic group (DM, a low dose of acarbose group (AcarL and a high dose of acarbose group (AcarH. Ileum samples were analyzed using miRCURY LNA™ microRNA Array, qPCR and immunohistochemistry. We found that 8-week treatment with acarbose significantly decreased fasting blood glucose. Oral glucose tolerance tests (OGTT showed that blood glucose was significantly reduced in the AcarL and AcarH groups at 30 min, 60 min and 120 min after oral glucose administration. We found that miR-151*, miR-10a-5p, miR-205, miR-17-5p, miR-145 and miR-664 were up-regulated in the AcarH group, while miR-541 and miR-135b were down-regulated. Through target gene analysis, real time PCR and immunohistochemistry verification, we found that these miRNAs suppressed the expression of proinflammatory cytokines [IL6 (interleukin 6 and TNF (tumor necrosis factor] and mitogen activated protein kinase 1 (MAPK1. Our data suggest that acarbose can improve blood glucose in diabetic rats through the MAPK pathway and can down-regulate proinflammatory factors by activating miR-10a-5p and miR-664 in the ileum.

  3. Anti-cancer stemness and anti-invasive activity of bitter taste receptors, TAS2R8 and TAS2R10, in human neuroblastoma cells.

    Science.gov (United States)

    Seo, Yoona; Kim, Yoo-Sun; Lee, Kyung Eun; Park, Tai Hyun; Kim, Yuri

    2017-01-01

    Neuroblastoma (NB) originates from immature neuronal cells and currently has a poor clinical outcome. NB cells possess cancer stem cells (CSCs) characteristics that facilitate the initiation of a tumor, as well as its metastasis. Human bitter taste receptors, referred to as TAS2Rs, are one of five types of basic taste receptors and they belong to a family of G-protein coupled receptors. The recent finding that taste receptors are expressed in non-gustatory tissues suggest that they mediate additional functions distinct from taste perception. While it is generally admitted that the recognition of bitter tastes may be associated with a self-defense system to prevent the ingestion of poisonous food compounds, this recognition may also serve as a disease-related function in the human body. In particular, the anti-cancer stemness and invasion effects of TAS2Rs on NB cells remain poorly understood. In the present study, endogenous expression of TAS2R8 and TAS2R10 in SK-N-BE(2)C and SH-SY5Y cells was examined. In addition, higher levels of TAS2R8 and TAS2R10 expression were investigated in more differentiated SY5Y cells. Both TAS2Rs were up-regulated following the induction of neuronal cell differentiation by retinoic acid. In addition, ectopic transfection of the two TAS2Rs induced neurite elongation in the BE(2)C cells, and down-regulated CSCs markers (including DLK1, CD133, Notch1, and Sox2), and suppressed self-renewal characteristics. In particular, TAS2RS inhibited tumorigenicity. Furthermore, when TAS2Rs was over-expressed, cell migration, cell invasion, and matrix metalloproteinases activity were inhibited. Expression levels of hypoxia-inducible factor-1α, a well-known regulator of tumor metastasis, as well as its downstream targets, vascular endothelial growth factor and glucose transporter-1, were also suppressed by TAS2Rs. Taken together, these novel findings suggest that TAS2Rs targets CSCs by suppressing cancer stemness characteristics and NB cell invasion

  4. Quercetin increases the bioavailability of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in rats

    NARCIS (Netherlands)

    Schutte, M.E.; Alink, G.M.; Freidig, A.P.; Spenkelink, B.; Vaessen, J.C.H.; Sandt, J.J.M. van de; Groten, J.P.; Rietjens, I.M.C.M.

    2008-01-01

    This study investigates whether the previous observation that quercetin increases the transport of PhIP through Caco-2 monolayers in vitro could be confirmed in an in vivo rat model. Co-administration of 1.45 μmol PhIP/kg bw and 30 μmol quercetin/kg bw significantly increased the blood AUC(0-8 h) of

  5. 中小型IP PBX系统结构的研究%Research on Medium and Small IP PBX Architecture

    Institute of Scientific and Technical Information of China (English)

    章仁龙; 周宇

    2005-01-01

    IP PBX(PBX over IP)是VoIP(Voice over IP)领域内的一个研究热点.IP PBX将企业内部现有的数据网与电话网融合,是企业与公共交换电话网PSTN(Public Switched Telephone Network)、IP网等外部网络通信的桥梁.本文基于现有的多个IP PBX系统抽象出两种最常见的模型,并在此基础上改进后,提出了一种适合中小型IP PBX系统的结构模型.

  6. NOVEL APPROACH TO RESOLVE NETWORK SECURITY ISSUES IN IP-PBX IN CONVERGED ARCHITECTURE

    Directory of Open Access Journals (Sweden)

    MUNIR B. SAYYAD,

    2010-10-01

    Full Text Available This paper aims to examine the security issues in the IP-PBX (IP Private Branch Exchange .The Traces are taken on the live environment using Vendor ‘X’ CDMA MSC. The traces are also taken at SBC (Session Boarder Controller. The PBX services can be provided over IP via a SIP (session initiation protocol trunk provided by network operator. The SIP trunk is connected to a switch i.e. MSC. Thus IP connectivity is available at IP-PBX end. Now calls can be originated from IP-PBX which is terminating on a mobile handset registered with MSC. The problem arises when a dummy/blank/fake CLI (caller line identification is configured at IP-PBX. As MSC isdoing only part of routing depending upon called party number, such calls with fake CLI pass through MSC without any intervention. So called party and even MSC are unaware of real number of calling party. Similar security issue arises when IP-PBX sends dummy IP addresses of IP phones connected to IP-PBX. Thus conflict of IP addresses and or called party numbers creates a major security concern. These are important issues for interfacing IP with traditional wire line or wireless network. Such a security issue can be resolved by registering IP-PBX and its extension numbers with MSC. This paper describes the probable methods to resolve above issues.

  7. Detection of a rare BCR-ABL tyrosine kinase fusion protein in H929 multiple myeloma cells using immunoprecipitation (IP)-tandem mass spectrometry (MS/MS).

    Science.gov (United States)

    Breitkopf, Susanne B; Yuan, Min; Pihan, German A; Asara, John M

    2012-10-02

    Hypothesis directed proteomics offers higher throughput over global analyses. We show that immunoprecipitation (IP)-tandem mass spectrometry (LC-MS/MS) in H929 multiple myeloma (MM) cancer cells led to the discovery of a rare and unexpected BCR-ABL fusion, informing a therapeutic intervention using imatinib (Gleevec). BCR-ABL is the driving mutation in chronic myeloid leukemia (CML) and is uncommon to other cancers. Three different IP-MS experiments central to cell signaling pathways were sufficient to discover a BCR-ABL fusion in H929 cells: phosphotyrosine (pY) peptide IP, p85 regulatory subunit of phosphoinositide-3-kinase (PI3K) IP, and the GRB2 adaptor IP. The pY peptides inform tyrosine kinase activity, p85 IP informs the activating adaptors and receptor tyrosine kinases (RTKs) involved in AKT activation and GRB2 IP identifies RTKs and adaptors leading to ERK activation. Integration of the bait-prey data from the three separate experiments identified the BCR-ABL protein complex, which was confirmed by biochemistry, cytogenetic methods, and DNA sequencing revealed the e14a2 fusion transcript. The tyrosine phosphatase SHP2 and the GAB2 adaptor protein, important for MAPK signaling, were common to all three IP-MS experiments. The comparative treatment of tyrosine kinase inhibitor (TKI) drugs revealed only imatinib, the standard of care in CML, was inhibitory to BCR-ABL leading to down-regulation of pERK and pS6K and inhibiting cell proliferation. These data suggest a model for directed proteomics from patient tumor samples for selecting the appropriate TKI drug(s) based on IP and LC-MS/MS. The data also suggest that MM patients, in addition to CML patients, may benefit from BCR-ABL diagnostic screening.

  8. Predicting Packet Transmission Data over IP Networks Using Adaptive Neuro-Fuzzy Inference Systems

    Directory of Open Access Journals (Sweden)

    Samira Chabaa

    2009-01-01

    Full Text Available Problem statement: The statistical modeling for predicting network traffic has now become a major tool used for network and is of significant interest in many domains: Adaptive application, congestion and admission control, wireless, network management and network anomalies. To comprehend the properties of IP-network traffic and system conditions, many kinds of reports based on measured network traffic data have been reported by several researchers. The goal of the present contribution was to complement these previous researches by predicting network traffic data. Approach: The Adaptive Neuro-Fuzzy Inference System (ANFIS was realized by an appropriate combination of fuzzy systems and neural networks. It was applied in different applications which have been increased in recent years and have multidisciplinary in several domains with a high accuracy. For this reason, we used a set of input and output data of packet transmission over Internet Protocol (IP networks as input and output of ANFIS to develop a model for predicting data. Results: ANFIS was compared with some existing model based on Volterra system with Laguerre functions. The obtained results demonstrate that the sequences of generated values have the same statistical characteristics as those really observed. Furthermore, the relative error using ANFIS model was better than this obtained by Volterra system model. Conclusion: The developed model fits well real data and can be used for predicting purpose with a high accuracy.

  9. b-CGC versus IP-Sat and high precision combined HERA data

    CERN Document Server

    Rezaeian, Amir H

    2014-01-01

    The Impact-Parameter dependent Color Glass Condensate (b-CGC) and Saturation (IP-Sat) dipole models incorporate key features of small-x physics properties and match smoothly to the perturbative QCD regime at large $Q^2$ for a given $x$. Although both models include saturation effects and depend on impact-parameter, the former is based on the non-linear BK equation, while the latter is based on DGLAP evolution. After confronting the models to the recently released high precision combined HERA data, we show that in both models, the typical impact-parameter probed in the total $\\gamma^{*}p$ cross-section is about $b\\approx 2\\div 3\\,\\text{GeV}^{-1}$ and the proton saturation scale is $Q_S<1$ GeV in HERA kinematics. We show that most features of inclusive DIS and exclusive diffractive data at HERA are correctly reproduced in both models. Nevertheless, the b-CGC and the IP-Sat models give significantly different predictions beyond the current HERA kinematics for the structure functions at very low x and high vir...

  10. Recent Research Progress in Green IP over WDM Network%绿色IP over WDM网络研究进展

    Institute of Scientific and Technical Information of China (English)

    郭爱煌; 薛琳

    2012-01-01

    近年来光网络朝着高速率、大带宽的方向迅猛发展,设备数目快速增加导致网络能耗急剧增长.为了降低网络能耗,提高能量利用率,绿色IP over WDM网络的概念被提出,并成为光网络领域的最新研究热点之一.对现有光网络节能方法的研究状况进行综述,从网络设备入手,讨论业务负载和设备能耗的关系,建立了两种能耗模型.基于IP over WDM网络结构,利用数学表达式描述了网络能耗最小化问题.对当前网络级的节能方法进行分类,分析了其基本原理和节能效果,并选取代表性的节能方法进行比较,得出了现有方法存在的局限性和不足,对进一步的研究方向提出了建议.%In recent years, optical network is developing towards high rate and wide bandwidth. As the number of network equipments in optical network increases, the network power consumption has a sharp rising. In order to decrease the energy consumption and improve the energy efficiency, the concept of green IP over WDM network has been proposed and it has become a hotspot for research in the area of optical network. The existing research status for energy-saving approaches in optical network is reviewed. Specifically, starting with network equipment, the relationship between traffic load and energy consumption of network equipments is discussed and two power consumption models are established. Then a mathematical expression of minimized energy consumption for IP over WDM network is introduced based on the network structure. Subsequently, the methods to reduce the energy consumption at network level are divided into different categories, whose fundamental principles and energy saving effects are summarized, and several typical energy-saving approaches are compared as well. Finally, the limitations of existing approaches are analyzed and some suggestions are provided for the further research directions.

  11. KSR-Based Medium Improves the Generation of High-Quality Mouse iPS Cells

    OpenAIRE

    2014-01-01

    Induced pluripotent stem (iPS) cells from somatic cells have great potential for regenerative medicine. The efficiency in generation of iPS cells has been significantly improved in recent years. However, the generation of high-quality iPS cells remains of high interest. Consistently, we demonstrate that knockout serum replacement (KSR)-based medium accelerates iPS cell induction and improves the quality of iPS cells, as confirmed by generation of chimeras and all iPS cell-derived offspring wi...

  12. [Retinal Cell Therapy Using iPS Cells].

    Science.gov (United States)

    Takahashi, Masayo

    2016-03-01

    Progress in basic research, starting with the work on neural stem cells in the middle 1990's to embryonic stem (ES) cells and induced pluripotent stem (iPS) cells at present, will lead the cell therapy (regenerative medicine) of various organs, including the central nervous system to a big medical field in the future. The author's group transplanted iPS cell-derived retinal pigment epithelial (RPE) cell sheets to the eye of a patient with exudative age-related macular degeneration (AMD) in 2014 as a clinical research. Replacement of the RPE with the patient's own iPS cell-derived young healthy cell sheet will be one new radical treatment of AMD that is caused by cellular senescence of RPE cells. Since it was the first clinical study using iPS cell-derived cells, the primary endpoint was safety judged by the outcome one year after surgery. The safety of the cell sheet has been confirmed by repeated tumorigenisity tests using immunodeficient mice, as well as purity of the cells, karyotype and genetic analysis. It is, however, also necessary to prove the safety by clinical studies. Following this start, a good strategy considering cost and benefit is needed to make regenerative medicine a standard treatment in the future. Scientifically, the best choice is the autologous RPE cell sheet, but autologous cell are expensive and sheet transplantation involves a risky part of surgical procedure. We should consider human leukocyte antigen (HLA) matched allogeneic transplantation using the HLA 6 loci homozyous iPS cell stock that Prof. Yamanaka of Kyoto University is working on. As the required forms of donor cells will be different depending on types and stages of the target diseases, regenerative medicine will be accomplished in a totally different manner from the present small molecule drugs. Proof of concept (POC) of photoreceptor transplantation in mouse is close to being accomplished using iPS cell-derived photoreceptor cells. The shortest possible course for treatment

  13. An in vitro and in silico study on the flavonoid-mediated modulation of the transport of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) through Caco-2 monolayers

    NARCIS (Netherlands)

    Schutte, M.E.; Freidig, A.P.; Sandt, J.J.M. van de; Alink, G.M.; Rietjens, I.M.C.M.; Groten, J.P.

    2006-01-01

    The present study describes the effect of different flavonoids on the absorption of the pro-carcinogen PhIP through Caco-2 monolayers and the development of an in silico model describing this process taking into account passive diffusion and active transport of PhIP. Various flavonoids stimulated th

  14. An in vitro and in silico study on the flavonoid mediated modulation of the transport of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) through Caco-2 monolayers

    NARCIS (Netherlands)

    Schutte, M.E.; Freidig, A.P.; Sandt, van de J.J.M.; Alink, G.M.; Rietjens, I.M.C.M.; Groten, J.P.

    2006-01-01

    The present study describes the effect of different flavonoids on the absorption of the pro-carcinogen PhIP through Caco-2 monolayers and the development of an in silico model describing this process taking into account passive diffusion and active transport of PhIP. Various flavonoids stimulated th

  15. Satellite communication on pipeline supervision using TCP-IP Protocol; Comunicacao via satelite na supervisao de dutos usando o protocolo TCP-IP

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Agliberto Pessoa da [PETROBRAS S.A., Rio de Janeiro, RJ (Brazil); Brunette, Sergio Henrique de Moraes

    2003-07-01

    Bit transparent type or X-25 protocols have been used in VSAT satellite communication by PETROBRAS pipeline Supervisory Control and Data Acquisition System (SCADA) as access protocol. Both solutions have inconveniences. By the first one, difficulties exist for analysis and diagnosis of connection flaws, and therefore, for a ready identification by the system management stations of abnormality location. On the other hand, the usage of the X-25 brings an additional over-head in the communication since this is not an available option in most of the equipment that compose the SCADA. The access to VSAT through Ethernet, in the opposite, makes available all networks management tools of the TCP/IP platform and it allows a direct connection to the field devices, since the main models of the several makers of Programmable Controllers and Remote Terminal Units have Ethernet port. An additional earnings is the possibility of standardization that this solution allows. This paper describes a series of communication tests between two Programmable Controllers communicating through the satellite, using a protocol over Ethernet/TCP/IP. (author)

  16. Prediction of permeability of cement-admixed soft clay using resistivity and time-domain IP measurements

    Science.gov (United States)

    Latt, Khin M. M.; Giao, P. H.

    2017-02-01

    Permeability is one of the most important petrophysical parameters, which unfortunately is quite difficult to be tested and estimated, particularly for the fine-grained soils and mixed soils. Prediction of permeability based on geophysical measurements is currently one of the most challenging issues in petrophysics. There have been recently reported some empirical relationships between permeability, resistivity and spectral induced polarization (SIP) parameters for a porous medium. However, the disadvantage of this approach is the very scarcity of SIP data as most of practical measurements are time-domain IP. In this study, a detailed overview of permeability prediction models using resistivity and spectral IP data was made. More than that, an innovative approach using resistivity and time-domain IP measurements to predict permeability of cement-admixed Bangkok clay was proposed and successfully applied for tested samples based on measurements of resistivity and time-domain IP data. A good amount of geotechnical and geophysical tests was conducted to investigate the time-dependent development of strength, porosity, and permeability of cement-mixed Bangkok soft clay samples during a 28-day curing process. The permeability predicted by resistivity and chargeability model matched well with permeability measured by consolidation testing. In addition, a series of correlations between unconfined compressive strength, porosity and permeability as measured by geotechnical testing and resistivity and chargeability as measured by geophysical testing were found.

  17. Effectiveness of Intrusion Prevention Systems (IPS) in Fast Networks

    CERN Document Server

    Shafi, Muhammad Imran; Hayat, Sikandar; Sohail, Imran

    2010-01-01

    Computer systems are facing biggest threat in the form of malicious data which causing denial of service, information theft, financial and credibility loss etc. No defense technique has been proved successful in handling these threats. Intrusion Detection and Prevention Systems (IDPSs) being best of available solutions. These techniques are getting more and more attention. Although Intrusion Prevention Systems (IPSs) show a good level of success in detecting and preventing intrusion attempts to networks, they show a visible deficiency in their performance when they are employed on fast networks. In this paper we have presented a design including quantitative and qualitative methods to identify improvement areas in IPSs. Focus group is used for qualitative analysis and experiment is used for quantitative analysis. This paper also describes how to reduce the responding time for IPS when an intrusion occurs on network, and how can IPS be made to perform its tasks successfully without effecting network speed nega...

  18. Topology-aware Overlay Multicast over IP Multicast Networks

    Institute of Scientific and Technical Information of China (English)

    CHEN Xiao; SHAO Hua-gang; WANG Wei-nong

    2008-01-01

    Most existing overlay multicast approaches refuse to consider any network layer support no matter whether it is available or not. This design principle greatly increases the complexity of the routing algorithms and makes the overlay topologies incompatible with the underlying network. To address these issues, topology-aware overlay multicast over IP multicast networks (TOMIMN) was proposed as a novel overlay multicast protocol, which exploits the cooperation between end-hosts and IP multicast routers to construct a topology-aware overlay tree. Through a little modification to protocol independent multicast sparse mode (PIM-SM),a multicast router is able to receive registration from nearby group members and redirect passing-by join re-quests to them. Due to the multicast router's support, TOMIMN organizes its group members into an overlay multicast tree efficiently, which matches the physical network topology well.

  19. Chaos Based Secure IP Communications over Satellite DVB

    Science.gov (United States)

    Caragata, Daniel; El Assad, Safwan; Tutanescu, Ion; Sofron, Emil

    2010-06-01

    The Digital Video Broadcasting—Satellite (DVB-S) standard was originally conceived for TV and radio broadcasting. Later, it became possible to send IP packets using encapsulation methods such as Multi Protocol Encapsulation, MPE, or Unidirectional Lightweight Encapsulation, ULE. This paper proposes a chaos based security system for IP communications over DVB-S with ULE encapsulation. The proposed security system satisfies all the security requirements while respecting the characteristics of satellite links, such as the importance of efficient bandwidth utilization and high latency time. It uses chaotic functions to generate the keys and to encrypt the data. The key management is realized using a multi-layer architecture. A theoretical analysis of the system and a simulation of FTP and HTTP traffic are presented and discussed to show the cost of the security enhancement and to provide the necessary tools for security parameters setup.

  20. Ionospheric Disturbances and their Impact on IPS Using MEXART Observations

    CERN Document Server

    Rodríguez-Martínez, Mario; Carrillo-Vargas, Armando; López-Montes, Rebeca; Araujo-Pradere, Eduardo A; Casillas-Pérez, Gilberto A; Cruz-Abeyro, José A L

    2014-01-01

    We study the impact of ionospheric disturbances on the Earth's environment caused by the solar events that occurred from 20 April to 31 May 2010, using observations from the Mexican Array Radio Telescope (MEXART). During this period of time, several astronomical sources presented fluctuations in their radio signals. Wavelet analysis, together with complementary information such as the vertical total electron content (vTEC) and the Dst index, were used to identify and understand when the interplanetary scintillation (IPS) could be contaminated by ionospheric disturbances (IOND). We find that radio signal perturbations were sometimes associated with IOND and/or IPS fluctuations; however, in some cases, it was not possible to clearly identify their origin. Our Fourier and wavelet analyses showed that these fluctuations had frequencies in the range $\\approx$ 0.01 Hz -- $\\approx$ 1.0 Hz (periodicities of 100 s to 1 s).

  1. Fault Tolerant Distributed and Fixed Hierarchical Mobile IP

    Directory of Open Access Journals (Sweden)

    Paramesh C. Upadhyay

    2010-04-01

    Full Text Available To several mobility management protocols proposed for IP-based mobile networks, faulttolerance aspect of mobility agents is a primary requirement to sustain continuous service availability to themobile hosts. For a localized or micro- mobility management solution, the local mobility agent i.e. gateway isa single point of failure because it is responsible for enforcing the signaling and data packets in its domain.Such failures may severely disrupt the communications among the failure-affected users. The problembecomes even more severe for mobility agents in a distributed mobility management scheme with overlappingregistration areas.This paper proposes a fault tolerance scheme for Distributed and Fixed Hierarchical Mobile IP(DFHMIP and evaluates its performance in terms of data transmission cost and blocking probability.

  2. Generation and Characterization of a MYF5 Reporter Human iPS Cell Line Using CRISPR/Cas9 Mediated Homologous Recombination.

    Science.gov (United States)

    Wu, Jianbo; Hunt, Samuel D; Xue, Haipeng; Liu, Ying; Darabi, Radbod

    2016-01-05

    Human iPS cells hold great promise for disease modeling and treatment of degenerative disorders including muscular dystrophies. Although a few research groups have used them for skeletal muscle differentiation, most were based on gene over-expression or long-term mesenchymal differentiation and retrospective identification of myogenic cells. Therefore, this study was aimed to generate a knock-in reporter human iPS cell line for MYF5, as an early myogenic specification gene, to allow prospective identification and purification of myogenic progenitors from human iPS cells. By using a CRISPR/Cas9 double nickase strategy, a 2A-GFP reporter was inserted before the stop codon of the MYF5 gene using homologous recombination. This approach allowed for highly efficient in-frame targeting of MYF5 in human iPS cells. Furthermore, in order to prove the reporter function, endogenous MYF5 expression was induced using a novel dead Cas9-VP160 transcriptional activator. Induced clones demonstrated appropriate MYF5-GFP co-expression. Finally, to confirm the differentiation potential, reporter human iPS clones were differentiated through embryoid body method and MYF5-GFP(+) myogenic cells were sorted and characterized. These data provides valuable guidelines for generation of knock-in reporter human iPS cell lines for myogenic genes which can be used for disease modeling, drug screening, gene correction and future in vivo applications.

  3. A Multimedia over IP Integrated System for Military Communications

    Science.gov (United States)

    2006-12-01

    Alessandro Cignoni, Carlo Roatta *Istituto per le Telecomunicazioni e l’Elettronica “Giancarlo Vallauri” Telecommunications Department Viale Italia n°72...for a real battlefield scenario. Cignoni, A.; Garroppo, R.G.; Martucci, A.; Roatta , C. (2006) A Multimedia over IP Integrated System for Military...October 2003. Rome, 18-19 April 2005 RTO-IST-054 Paper 14 Alessandro CIGNONI, Rosario G. GARROPPO, Alessandro MARTUCCI, Carlo ROATTA A Multimedia

  4. Implementation of Cloud Computing into VoIP

    Directory of Open Access Journals (Sweden)

    Floriana GEREA

    2012-08-01

    Full Text Available This article defines Cloud Computing and highlights key concepts, the benefits of using virtualization, its weaknesses and ways of combining it with classical VoIP technologies applied to large scale businesses. The analysis takes into consideration management strategies and resources for better customer orientation and risk management all for sustaining the Service Level Agreement (SLA. An important issue in cloud computing can be security and for this reason there are several security solution presented.

  5. At-Speed Logic BIST for IP Cores

    CERN Document Server

    Cheon, B; Wang, L -T; Wen, X; Hsu, P; Cho, J; Park, J; Chao, H; Wu, S

    2011-01-01

    This paper describes a flexible logic BIST scheme that features high fault coverage achieved by fault-simulation guided test point insertion, real at-speed test capability for multi-clock designs without clock frequency manipulation, and easy physical implementation due to the use of a low-speed SE signal. Application results of this scheme to two widely used IP cores are also reported.

  6. TCP/IP Security%TCP/IP协议安全

    Institute of Scientific and Technical Information of China (English)

    张少中; 唐毅谦; 赵悦; 聂铁铸; 刘丽华

    2000-01-01

    TCP/IP协议是Internet上网络互连的基础,TCP/IP 协议的安全直接关系到网络和系统的安全.本文通过分析TCP/IP的工作机制,讨论了网络入侵的基本途径,并阐述了一些改进和提高TCP/IP协议安全性的方法.

  7. TCP/IP Sockets in C# Practical Guide for Programmers

    CERN Document Server

    Makofske, David; Calvert, Kenneth L

    2004-01-01

    "TCP/IP sockets in C# is an excellent book for anyone interested in writing network applications using Microsoft .Net frameworks. It is a unique combination of well written concise text and rich carefully selected set of working examples. For the beginner of network programming, it''s a good starting book; on the other hand professionals could also take advantage of excellent handy sample code snippets and material on topics like message parsing and asynchronous programming."Adarsh Khare, SDT, .Net Frameworks Team, Microsoft CorporationThe popularity of the C# language and the .NET framework is ever rising due to its ease of use, the extensive class libraries available in the .NET Framework, and the ubiquity of the Microsoft Windows operating system, to name a few advantages. TCP/IP Sockets in C# focuses on the Sockets API, the de facto standard for writing network applications in any programming language. Starting with simple client and server programs that use TCP/IP (the Internet protocol suite), students ...

  8. A Framework for an IP-Based DVB Transmission Network

    Directory of Open Access Journals (Sweden)

    Nimbe L. Ewald-Arostegui

    2010-01-01

    Full Text Available One of the most important challenges for next generation all-IP networks is the convergence and interaction of wireless and wired networks in a smooth and efficient manner. This challenge will need to be faced if broadcast transmission networks are to converge with IP infrastructure. The 2nd generation of DVB standards supports the Generic Stream, allowing the direct transmission of IP-based content using the Generic Stream Encapsulation (GSE, in addition to the native Transport Stream (TS. However, the current signalling framework is based on MPEG-2 Tables that rely upon the TS. This paper examines the feasibility of providing a GSE signalling framework, eliminating the need for the TS. The requirements and potential benefits of this new approach are described. It reviews prospective methods that may be suitable for network discovery and selection and analyses different options for the transport and syntax of this signalling metadata. It is anticipated that the design of a GSE-only signalling system will enable DVB networks to function as a part of the Internet.

  9. IP Pegasi in outburst: Echelle spectroscopy & Modulation Doppler Tomography

    CERN Document Server

    Papadaki, C; Steeghs, D

    2008-01-01

    We analyse a unique set of time-resolved echelle spectra of the dwarf nova IP Peg, obtained at ESO's NTT with EMMI. The dataset covers the wavelength range of 4000-7500A and shows Balmer, HeI, HeII and heavier elements in emission. IP Peg was observed one day after the peak of an outburst. The trailed spectra, spectrograms and Doppler maps show characteristics typical of IP Pegasi during the early stages of its outburst. The high-ionisation line of HeII 4686A is the most centrally located line and has the greatest radial extension compared to the HeI lines. The Balmer lines extend from close to the white dwarf up to approximately 0.45 times R_L, with the outer radius gradually increasing when moving from H delta to H alpha. The application, for the first time, of the modulation Doppler tomography technique, maps any harmonically varying components present in the system configuration. We find, as expected, that part of the strong secondary star emission in Balmer and HeI lines is modulated predominantly with t...

  10. Induction of iPS cells and of cancer stem cells: the stem cell or reprogramming hypothesis of cancer?

    Science.gov (United States)

    Trosko, James E

    2014-01-01

    This article as designed to examine whether the "stoichiometric" or "elite models" of the origin of the "induced pluripotent stem" (iPS) cells fits some experiment facts from the developmental biology of adult stem cells and from the field of cancer research. In brief, since the evidence presented to support the stoichiometric model failed to recognize the factual existence of adult organ specific stem cells, the model has not been rigorously tested. In addition, the demonstration of a subset of cells (MUSE cells) in normal primary in vitro cultures of human fibroblasts (the usual source of iPS cells) seems to be the origin of the iPS cells. Moreover, from the field of carcinogenesis, the "stem cell" versus "de-differentiation" or "reprogramming" hypotheses were examined. Again, using the role of glycolysis, known to be associated with the Warburg effect in cancer cells, a list of experiments showing that (a) normal stem cells, which have few mitochondria, metabolize via glycolysis; (b) the stem cells are targets for "initiation" or "immortalization" or the blockage of differentiation and apoptosis of the stem cells by "immortalizing viruses"; (c) Lactate dehydrogenase A (LDHA), when expressed, is associated with glycolysis and therefore, must be expressed in normal adult stem cells, as well as in cancer cells; and (d) p53, depleted or rendered dysfunctional by SV40 Large T antigen, is associated with the reduction of mitochondrial function and mass and is associated with the Warburg effect. Together, these observations from the iPS and "cancer stem cell" fields support the idea that both iPS cells and cancer stem cell are derived from adult organ-specific stem cells that do not restore or switch their metabolism of glucose from oxidative metabolism to glycolysis but, rather, in both cases, the adult stem cell, which metabolizes by glycolysis, is prevented from differentiation or from metabolizing by oxidative phosphorylation.

  11. Probe into and Analysis of Ways of Resolving IP Right Conflicts

    Institute of Scientific and Technical Information of China (English)

    Li; Yonghong

    2005-01-01

      The conflict of the IP rights has become an unavoid able contradiction. To duly settle the various contradictions, account should be taken not only of the balance of interests among different rightholders and between the rightholders and the public, but also of factors, such as implementability. To this writer, in respect of some models of resolution under the current law, reexamination should be conducted from the perspective of the attribute of the rights, rationality of the public policy and implementability of law, so as to seek more reasonable approach to conflict resolution.……

  12. Probe into and Analysis of Ways of Resolving IP Right Conflicts

    Institute of Scientific and Technical Information of China (English)

    Li Yonghong

    2005-01-01

    @@ The conflict of the IP rights has become an unavoid able contradiction. To duly settle the various contradictions, account should be taken not only of the balance of interests among different rightholders and between the rightholders and the public, but also of factors, such as implementability. To this writer, in respect of some models of resolution under the current law, reexamination should be conducted from the perspective of the attribute of the rights, rationality of the public policy and implementability of law, so as to seek more reasonable approach to conflict resolution.

  13. On the implementation of IP protection using biometrics based information hiding and firewall

    Science.gov (United States)

    Basu, Abhishek; Nandy, Kingshuk; Banerjee, Avishek; Giri, Supratick; Sarkar, Souvik; Sarkar, Subir Kumar

    2016-02-01

    System-on-chip-based design style creates a revolution in very large scale integration industry with design efficiency, operating speed and development time. To support this process, reuse and exchange of components are essential in electronic form called intellectual property (IP). This, however, increases the possibility of encroachment of IP of the design. So copyright protection of IP against piracy is the most important concern for IP vendors. The existing solutions for IP protection are still not secure enough with flexibility, cost, etc. This paper proposes an information-hiding-based solution for IP protection by embedding a biometric copyright information and firewall inside an IP in the form of a finite state machine with unique configuration. The scheme first introduces biometric signature-based copyright as ownership proof. Second, firewall interrupts the normal functionality of IP at the end of the user time period. The experimental outcomes of field-programmable-gate-array implementation illustrate the efficiency of the proposed method.

  14. Download - ChIP-Atlas | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available switchLanguage; BLAST Search Image Search Home About Archive Update History Data List Contact us ChIP-Atla...tory of This Database Site Policy | Contact Us Download - ChIP-Atlas | LSDB Archive ...

  15. A Study of the Next Generation Intelligent Networks Interworking with IP Networks

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    In the past three years, the author has participated in several projects involved in Intelligent Network (IN). In 1997, we started our research on the project “The Integration of IN and B-ISDN” supported by National Science Foundation. Since 1999, the author has engaged in the project “the Next Generation IN Based Service Platform Interworking with IP Networks” supported by the National 863 Project. While working on the project, the author made a deep research on the architecture and the key technology of the next generation IN defined by ITU-T IN CS3 and CS4. In the following parts. 1. I conclude the research work and the innovation in my dissertation. The early research on the interworking of IN and Internet, despite the shortage of materials, the interworking architecture by means of IAF is analyzed. Some key techniques like triggering intelligent service and reporting dynamic IP address are solved. Some new SIBs and INAPs are added, and the dial-up software of users is enhanced, too. The incoming call notification service is designed. What we have done paves the way for the future research. 2. The gateway based architecture for the interworking of IN and Internet is described. Considering the research results from ITU, PINT work group and TIPHON work group, we emphasize that the interworking scenario through MG,SCG and C/BG is promising. Based on the CIN software developed by ourselves, we provide the scenario to enhance the function entities and signaling protocol. We address the function of entities and the interaction of signaling with an example like ICW service. 3. With the introduction of mobile agent technology, the author proposes the distributed IN which can be integrated with IP networks. According to the drawbacks of the centralized IN, we address the architecture and conceptual model of distributed IN to support advanced service in IP Telephony. We try to illustrate the role and relationship between function entities. We also present a

  16. DNA-binding proteins regulating pIP501 transfer and replication

    Directory of Open Access Journals (Sweden)

    Elisabeth Grohmann

    2016-08-01

    Full Text Available pIP501 is a Gram-positive broad-host-range model plasmid intensively used for studying plasmid replication and conjugative transfer. It is a multiple antibiotic resistance plasmid frequently found in clinical Enterococcus faecalis and Enterococcus faecium isolates. Replication of pIP501 proceeds unidirectionally by a theta mechanism. The minimal replicon of pIP501 is composed of the repR gene encoding the essential rate-limiting replication initiator protein RepR and the origin of replication, oriR, located downstream of repR. RepR is similar to RepE of related streptococcal plasmid pAMβ1, which has been shown to possess RNase activity cleaving free RNA molecules in close proximity of the initiation site of DNA synthesis. Replication of pIP501 is controlled by the concerted action of a small protein, CopR, and an antisense RNA, RNAIII. CopR has a dual role: It acts as transcriptional repressor at the repR promoter and prevents convergent transcription of RNAIII and repR mRNA (RNAII, thereby indirectly increasing RNAIII synthesis. CopR binds asymmetrically as a dimer at two consecutive binding sites upstream of and overlapping with the repR promoter. RNAIII induces transcriptional attenuation within the leader region of the repR mRNA (RNAII. Deletion of either control component causes a 10- to 20-fold increase of plasmid copy number, while simultaneous deletions have no additional effect. Conjugative transfer of pIP501 depends on a type IV secretion system (T4SS encoded in a single operon. Its transfer host-range is considerably broad, as it has been transferred to virtually all Gram-positive bacteria including filamentous streptomycetes and even the Gram-negative Escherichia coli. Expression of the 15 genes encoding the T4SS is tightly controlled by binding of the relaxase TraA, the transfer initiator protein, to the operon promoter, which overlaps with the origin of transfer (oriT. The T4SS operon encodes the DNA-binding proteins TraJ (VirD4

  17. DNA-Binding Proteins Regulating pIP501 Transfer and Replication

    Science.gov (United States)

    Grohmann, Elisabeth; Goessweiner-Mohr, Nikolaus; Brantl, Sabine

    2016-01-01

    pIP501 is a Gram-positive broad-host-range model plasmid intensively used for studying plasmid replication and conjugative transfer. It is a multiple antibiotic resistance plasmid frequently detected in clinical Enterococcus faecalis and Enterococcus faecium strains. Replication of pIP501 proceeds unidirectionally by a theta mechanism. The minimal replicon of pIP501 is composed of the repR gene encoding the essential rate-limiting replication initiator protein RepR and the origin of replication, oriR, located downstream of repR. RepR is similar to RepE of related streptococcal plasmid pAMβ1, which has been shown to possess RNase activity cleaving free RNA molecules in close proximity of the initiation site of DNA synthesis. Replication of pIP501 is controlled by the concerted action of a small protein, CopR, and an antisense RNA, RNAIII. CopR has a dual function: It acts as transcriptional repressor at the repR promoter and, in addition, prevents convergent transcription of RNAIII and repR mRNA (RNAII), which indirectly increases RNAIII synthesis. CopR binds asymmetrically as a dimer at two consecutive binding sites upstream of and overlapping with the repR promoter. RNAIII induces transcriptional attenuation within the leader region of the repR mRNA (RNAII). Deletion of either control component causes a 10- to 20-fold increase of plasmid copy number, while simultaneous deletions have no additional effect. Conjugative transfer of pIP501 depends on a type IV secretion system (T4SS) encoded in a single operon. Its transfer host-range is considerably broad, as it has been transferred to virtually all Gram-positive bacteria including Streptomyces and even the Gram-negative Escherichia coli. Expression of the 15 genes encoding the T4SS is tightly controlled by binding of the relaxase TraA, the transfer initiator protein, to the operon promoter overlapping with the origin of transfer (oriT). The T4SS operon encodes the DNA-binding proteins TraJ (VirD4-like coupling

  18. Annotation of gene promoters by integrative data-mining of ChIP-seq Pol-II enrichment data.

    Science.gov (United States)

    Gupta, Ravi; Wikramasinghe, Priyankara; Bhattacharyya, Anirban; Perez, Francisco A; Pal, Sharmistha; Davuluri, Ramana V

    2010-01-18

    Use of alternative gene promoters that drive widespread cell-type, tissue-type or developmental gene regulation in mammalian genomes is a common phenomenon. Chromatin immunoprecipitation methods coupled with DNA microarray (ChIP-chip) or massive parallel sequencing (ChIP-seq) are enabling genome-wide identification of active promoters in different cellular conditions using antibodies against Pol-II. However, these methods produce enrichment not only near the gene promoters but also inside the genes and other genomic regions due to the non-specificity of the antibodies used in ChIP. Further, the use of these methods is limited by their high cost and strong dependence on cellular type and context. We trained and tested different state-of-art ensemble and meta classification methods for identification of Pol-II enriched promoter and Pol-II enriched non-promoter sequences, each of length 500 bp. The classification models were trained and tested on a bench-mark dataset, using a set of 39 different feature variables that are based on chromatin modification signatures and various DNA sequence features. The best performing model was applied on seven published ChIP-seq Pol-II datasets to provide genome wide annotation of mouse gene promoters. We present a novel algorithm based on supervised learning methods to discriminate promoter associated Pol-II enrichment from enrichment elsewhere in the genome in ChIP-chip/seq profiles. We accumulated a dataset of 11,773 promoter and 46,167 non-promoter sequences, each of length 500 bp, generated from RNA Pol-II ChIP-seq data of five tissues (Brain, Kidney, Liver, Lung and Spleen). We evaluated the classification models in building the best predictor and found that Bagging and Random Forest based approaches give the best accuracy. We implemented the algorithm on seven different published ChIP-seq datasets to provide a comprehensive set of promoter annotations for both protein-coding and non-coding genes in the mouse genome. The

  19. PARP1 genomics: chromatin immunoprecipitation approach using anti-PARP1 antibody (ChIP and ChIP-seq).

    Science.gov (United States)

    Lodhi, Niraj; Tulin, Alexei V

    2011-01-01

    Poly(ADP-ribose) polymerase1 (PARP1) is a global regulator of different cellular mechanisms, ranging from DNA damage repair to control of gene expression. Since PARP1 protein and pADPr have been shown to persist in chromatin through cell cycle, they may both act as epigenetic markers. However, it is not known how many loci are occupied by PARP1 protein during mitosis genome-wide. To reveal the genome-wide PARP1 binding sites, we used the ChIP-seq approach, an emerging technique to study genome-wide PARP1 protein interaction with chromatin. Here, we describe how to perform ChIP-seq in the context of PARP1 binding sites identification in chromatin, using human embryonic kidney cell lines.

  20. Genomic imprinting is variably lost during reprogramming of mouse iPS cells

    OpenAIRE

    2013-01-01

    Derivation of induced pluripotent stem (iPS) cells is mainly an epigenetic reprogramming process. It is still quite controversial how genomic imprinting is reprogrammed in iPS cells. Thus, we derived multiple iPS clones from genetically identical mouse somatic cells. We found that parentally inherited imprint was variably lost among these iPS clones. Concurrent with the loss of DNA methylation imprint at the corresponding Snrpn and Peg3 imprinted regions, parental origin-specific expression o...