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Sample records for model dentin adhesives

  1. Improved degree of conversion of model self-etching adhesives through their interaction with dentin

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    Zhang, Ying; Wang, Yong

    2011-01-01

    Objective To investigate the correlation of the chemical interaction between model self-etching adhesives and dentin with the degree of conversion (DC) of the adhesives. Methods The model self-etching adhesives contained bis[2-methacryloyloxy)ethyl] phosphate (2MP) and 2-hydroxyethyl methacrylate (HEMA) with a mass ratio of 1/1, and 0-40% water contents, respectively. The adhesives were applied either onto the prepared dentin surface or unreactive substrates (such as glass slides), agitated for 15s, then light-cured for 40s. The DCs of the adhesives were determined using micro-Raman spectral and mapping analysis. Results The DCs of the adhesives cured on the dentin substrate were found to be significantly higher than those on the unreactive glass substrate. Moreover, the DCs of the adhesives displayed a decreasing trend as the distance from the dentin surface became greater. The chemical interaction of the acidic 2MP/HEMA adhesives with the mineral apatite in dentin was proposed to play a significant role for the observations. The chemical interaction could be validated by the spectral comparison in the phosphate regions of 1100 cm−1 and 960 cm−1 in the Raman spectra. The results also revealed a notable influence of water content on the DC of adhesives. The DCs of the adhesive at 10% water content exhibited the highest DC level for both substrates. Conclusions Interaction with dentin dramatically improved the degree of conversion of self-etching adhesives. Our ability to chemically characterize the a/d interface including in situ detection of the DC distribution is very important in understanding self-etching adhesive bonding under in vivo conditions. PMID:22024375

  2. Improved degree of conversion of model self-etching adhesives through their interaction with dentine.

    Science.gov (United States)

    Zhang, Ying; Wang, Yong

    2012-01-01

    To investigate the correlation of the chemical interaction between model self-etching adhesives and dentine with the degree of conversion (DC) of the adhesives. The model self-etching adhesives contained bis[2-methacryloyloxy)ethyl] phosphate (2MP) and 2-hydroxyethyl methacrylate (HEMA) with a mass ratio of 1/1, and 0-40% water contents, respectively. The adhesives were applied either onto the prepared dentine surface or unreactive substrates (such as glass slides), agitated for 15s, then light-cured for 40s. The DCs of the adhesives were determined using micro-Raman spectral and mapping analysis. The DCs of the adhesives cured on the dentine substrate were found to be significantly higher than those on the unreactive glass substrate. Moreover, the DCs of the adhesives displayed a decreasing trend as the distance from the dentine surface became greater. The chemical interaction of the acidic 2MP/HEMA adhesives with the mineral apatite in dentine was proposed to play a significant role for the observations. The chemical interaction could be validated by the spectral comparison in the phosphate regions of 1100 cm(-1) and 960 cm(-1) in the Raman spectra. The results also revealed a notable influence of water content on the DC of adhesives. The DCs of the adhesive at 10% water content exhibited the highest DC level for both substrates. Interaction with dentine dramatically improved the degree of conversion of self-etching adhesives. Our ability to chemically characterise the a/d interface including in situ detection of the DC distribution is very important in understanding self-etching adhesive bonding under in vivo conditions. Copyright © 2011 Elsevier Ltd. All rights reserved.

  3. Polymer Nanocarriers for Dentin Adhesion

    Science.gov (United States)

    Osorio, R.; Osorio, E.; Medina-Castillo, A.L.; Toledano, M.

    2014-01-01

    To obtain more durable adhesion to dentin, and to protect collagen fibrils of the dentin matrix from degradation, calcium- and phosphate-releasing particles have been incorporated into the dental adhesive procedure. The aim of the present study was to incorporate zinc-loaded polymeric nanocarriers into a dental adhesive system to facilitate inhibition of matrix metalloproteinases (MMPs)-mediated collagen degradation and to provide calcium ions for mineral deposition within the resin-dentin bonded interface. PolymP-nActive nanoparticles (nanoMyP) were zinc-loaded through 30-minute ZnCl2 immersion and tested for bioactivity by means of 7 days’ immersion in simulated body fluid solution (the Kokubo test). Zinc-loading and calcium phosphate depositions were examined by scanning and transmission electron microscopy, elemental analysis, and x-ray diffraction. Nanoparticles in ethanol solution infiltrated into phosphoric-acid-etched human dentin and Single Bond (3M/ESPE) were applied to determine whether the nanoparticles interfered with bonding. Debonded sticks were analyzed by scanning electron microscopy. A metalloproteinase collagen degradation assay was also performed in resin-infiltrated dentin with and without nanoparticles, measuring C-terminal telopeptide of type I collagen (ICTP) concentration in supernatants, after 4 wk of immersion in artificial saliva. Numerical data were analyzed by analysis of variance (ANOVA) and Student-Newman-Keuls multiple comparisons tests (p calcium regardless of zinc incorporation. Nanoparticles failed to infiltrate demineralized intertubular dentin and remained on top of the hybrid layer, without altering bond strength. Calcium and phosphorus were found covering nanoparticles at the hybrid layer, after 24 h. Nanoparticle application in etched dentin also reduced MMP-mediated collagen degradation. Tested nanoparticles may be incorporated into dental adhesive systems to provide the appropriate environment in which dentin MMP

  4. Quantitative analysis of aqueous phase composition of model dentin adhesives experiencing phase separation

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    Ye, Qiang; Park, Jonggu; Parthasarathy, Ranganathan; Pamatmat, Francis; Misra, Anil; Laurence, Jennifer S.; Marangos, Orestes; Spencer, Paulette

    2013-01-01

    There have been reports of the sensitivity of our current dentin adhesives to excess moisture, for example, water-blisters in adhesives placed on over-wet surfaces, and phase separation with concomitant limited infiltration of the critical dimethacrylate component into the demineralized dentin matrix. To determine quantitatively the hydrophobic/hydrophilic components in the aqueous phase when exposed to over-wet environments, model adhesives were mixed with 16, 33, and 50 wt % water to yield well-separated phases. Based upon high-performance liquid chromatography coupled with photodiode array detection, it was found that the amounts of hydrophobic BisGMA and hydrophobic initiators are less than 0.1 wt % in the aqueous phase. The amount of these compounds decreased with an increase in the initial water content. The major components of the aqueous phase were hydroxyethyl methacrylate (HEMA) and water, and the HEMA content ranged from 18.3 to 14.7 wt %. Different BisGMA homologues and the relative content of these homologues in the aqueous phase have been identified; however, the amount of crosslinkable BisGMA was minimal and, thus, could not help in the formation of a crosslinked polymer network in the aqueous phase. Without the protection afforded by a strong crosslinked network, the poorly photoreactive compounds of this aqueous phase could be leached easily. These results suggest that adhesive formulations should be designed to include hydrophilic multimethacrylate monomers and water compatible initiators. PMID:22331596

  5. Prevention of root caries with dentin adhesives.

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    Grogono, A L; Mayo, J A

    1994-04-01

    This in vitro investigation determined the feasibility of using dentin adhesives to protect root surfaces against caries. The roots of 22 recently extracted human teeth were all painted with a protective lacquer leaving two unprotected small windows. On each specimen, one window (control) was left untreated and the other window (experimental) was treated using a dentin adhesive (Scotchbond Multi-Purpose). The roots were then immersed in an in vitro acetate/calcium/phosphate demineralization model at pH 4.3. After 70 days, the samples were removed and sectioned through the windows. The undecalcified ground sections were examined under transmitted and polarized light. Lesions characteristic of natural root caries were seen in the untreated control windows. No such lesions were apparent in the experimental windows. The results of this preliminary study suggest that dentin adhesives may provide protection against root caries.

  6. Bonding stability of adhesive systems to eroded dentin

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    Janaina Barros CRUZ

    2015-01-01

    Full Text Available This in vitro study evaluated the immediate and 6 months microshear bond strength (µSBS of different adhesive systems to sound and eroded dentin. Sixty bovine incisors were embedded in acrylic resin and ground to obtain flat buccal dentin surfaces. Specimens were randomly allocated into two groups: sound dentin (immersion in artificial saliva and eroded dentin (erosive challenge following a pH cycling model comprising 4 ×/day Sprite Light® drink for 10 days. Then, specimens were reassigned according to the adhesive system: etch-and-rinse adhesive (Adper Single Bond, two-step self-etch system (Clearfil SE Bond, or one-step self-etch adhesive (Adper Easy One. Polyethylene tubes with an internal diameter of 0.76 mm were placed over pre-treated dentin and filled with resin composite (Z250. Half of the specimens were evaluated by the µSBS test after 24 h, and the other half 6 months later, after water storage at 37°C. Failure mode was evaluated using a stereomicroscope (400×. Data were analyzed by three-way repeated measures analysis of variance and Tukey’s post hoc tests (α = 0.05. After 6 months of water aging, marked reductions in µSBS values were observed, irrespective of the substrate. The µSBS values for eroded dentin were lower than those obtained for sound dentin. No difference in bonding effectiveness was observed among adhesive systems. For all groups, adhesive/mixed failure was observed. In conclusion, eroded dentin compromises the bonding quality of adhesive systems over time.

  7. Eroded dentin does not jeopardize the bond strength of adhesive restorative materials

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    Janaina Barros Cruz

    2012-08-01

    Full Text Available This in vitro study evaluated the bond strength of adhesive restorative materials to sound and eroded dentin. Thirty-six bovine incisors were embedded in acrylic resin and ground to obtain flat buccal dentin surfaces. Specimens were randomly allocated in 2 groups: sound dentin (immersion in artificial saliva and eroded dentin (pH cycling model - 3× / cola drink for 7 days. Specimens were then reassigned according to restorative material: glass ionomer cement (KetacTM Molar Easy Mix, resin-modified glass ionomer cement (VitremerTM or adhesive system with resin composite (Adper Single Bond 2 + Filtek Z250. Polyethylene tubes with an internal diameter of 0.76 mm were placed over the dentin and filled with the material. The microshear bond test was performed after 24 h of water storage at 37ºC. The failure mode was evaluated using a stereomicroscope (400×. Bond strength data were analyzed with two-way ANOVA and Tukey's post hoc tests (α = 0.05. Eroded dentin showed bond strength values similar to those for sound dentin for all materials. The adhesive system showed the highest bond strength values, regardless of the substrate (p < 0.0001. For all groups, the adhesive/mixed failure prevailed. In conclusion, adhesive materials may be used in eroded dentin without jeopardizing the bonding quality. It is preferable to use an etch-and-rinse adhesive system because it shows the highest bond strength values compared with the glass ionomer cements tested.

  8. Adhesion of resin composite core materials to dentin.

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    O'Keefe, K L; Powers, J M

    2001-01-01

    This study determined (1) the effect of polymerization mode of resin composite core materials and dental adhesives on the bond strength to dentin, and (2) if dental adhesives perform as well to dentin etched with phosphoric acid as to dentin etched with self-etching primer. Human third molars were sectioned 2 mm from the highest pulp horn and polished. Three core materials (Fluorocore [dual cured], Core Paste [self-cured], and Clearfil Photo Core [light cured]) and two adhesives (Prime & Bond NT Dual Cure and Clearfil SE Bond [light cured]) were bonded to dentin using two dentin etching conditions. After storage, specimens were debonded in microtension and bond strengths were calculated. Scanning electron micrographs of representative bonding interfaces were analyzed. Analysis showed differences among core materials, adhesives, and etching conditions. Among core materials, dual-cured Fluorocore had the highest bond strengths. There were incompatibilities between self-cured Core Paste and Prime & Bond NT in both etched (0 MPa) and nonetched (3.0 MPa) dentin. Among adhesives, in most cases Clearfil SE Bond had higher bond strengths than Prime & Bond NT and bond strengths were higher to self-etched than to phosphoric acid-etched dentin. Scanning electron micrographs did not show a relationship between resin tags and bond strengths. There were incompatibilities between a self-cured core material and a dual-cured adhesive. All other combinations of core materials and adhesives produced strong in vitro bond strengths both in the self-etched and phosphoric acid-etched conditions.

  9. Adhesive sealing of dentin surfaces in vitro: A review

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    Abu-Nawareg, Manar M; Zidan, Ahmed Z; Zhou, Jianfeng; Agee, Kelli; Chiba, Ayaka; Tagami, Jungi; Pashley, David H

    2016-01-01

    Purpose The purpose of this review is to describe the evolution of the use of dental adhesives to form a tight seal of freshly prepared dentin to protect the pulp from bacterial products, during the time between crown preparation and final cementum of full crowns. The evolution of these “immediate dentin sealants” follows the evolution of dental adhesives, in general. That is, they began with multiple-step, etch-and-rinse adhesives, and then switched to the use of simplified adhesives. Methods Literature was reviewed for evidence that bacteria or bacterial products diffusing across dentin can irritate pulpal tissues before and after smear layer removal. Smear layers can be solubilized by plaque organisms within 7–10 days if they are directly exposed to oral fluids. It is likely that smear layers covered by temporary restorations may last more than one month. As long as smear layers remain in place, they can partially seal dentin. Thus, many in vitro studies evaluating the sealing ability of adhesive resins use smear layer-covered dentin as a reference condition. Surprisingly, many adhesives do not seal dentin as well as do smear layers. Results Both in vitro and in vivo studies show that resin-covered dentin allows dentinal fluid to cross polymerized resins. The use of simplified single bottle adhesives to seal dentin was a step backwards. Currently, most authorities use either 3-step adhesives such as Scotchbond Multi-Purposea or OptiBond FLb or two-step self-etching primer adhesives, such as Clearfil SEc, Unifil Bondd or AdheSEe, respectfully. PMID:26846037

  10. Effects of endodontic tri-antibiotic paste on bond strengths of dentin adhesives to coronal dentin

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    Parvin Mirzakoucheki

    2015-05-01

    Full Text Available Objectives The aim of this study was to evaluate the effects of tri-antibiotic paste (TAP on microtensile bond strengths (MTBS of dental adhesives to dentin. Materials and Methods Sixty extracted molars had their occlusal surfaces flattened to expose dentin. They were divided into two groups, i.e., control group with no dentin treatment and experimental group with dentin treatment with TAP. After 10 days, specimens were bonded using self-etch (Filtek P90 adhesive or etch-and-rinse (Adper Single Bond Plus adhesives and restored with composite resin. Teeth were sectioned into beams, and the specimens were subjected to MTBS test. Data were analyzed using two-way ANOVA and post hoc Tukey tests. Results There was a statistically significant interaction between dentin treatment and adhesive on MTBS to coronal dentin (p = 0.003. Despite a trend towards worse MTBS being noticed in the experimental groups, TAP application showed no significant effect on MTBS (p = 0.064. Conclusions The etch-and-rinse adhesive Adper Single Bond Plus presented higher mean bond strengths than the self-etch adhesive Filtek P90, irrespective of the group. The superior bond performance for Adper Single Bond when compared to Filtek P90 adhesive was confirmed by a fewer number of adhesive failures. The influence of TAP in bond strength is insignificant.

  11. Polymerization contraction stress in dentin adhesives bonded to dentin and enamel

    NARCIS (Netherlands)

    Hashimoto, M.; de Gee, A.J.; Feilzer, A.J.

    2008-01-01

    Objective In a previous study on of polymerization contraction stress determinations of adhesives bonded to dentin a continuous decline of stress was observed after the adhesives had been light-cured. The decline was ascribed to stress relief caused by diffusion into the adhesive layer of water

  12. Effects of matrix metallproteinases on dentin bonding and strategies to increase durability of dentin adhesion

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    Jung-Hyun Lee

    2012-02-01

    Full Text Available The limited durability of resin-dentin bonds severely compromises the longevity of composite resin restorations. Resin-dentin bond degradation might occur via degradation of water-rich and resin sparse collagen matrices by host-derived matrix metalloproteinases (MMPs. This review article provides overview of current knowledge of the role of MMPs in dentin matrix degradation and four experimental strategies for extending the longevity of resin-dentin bonds. They include: (1 the use of broad-spectrum inhibitors of MMPs, (2 the use of cross-linking agents for silencing the activities of MMPs, (3 ethanol wet-bonding with hydrophobic resin, (4 biomimetic remineralization of water-filled collagen matrix. A combination of these strategies will be able to overcome the limitations in resin-dentin adhesion.

  13. The effect of air thinning on dentin adhesive bond strength.

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    Hilton, T J; Schwartz, R S

    1995-01-01

    The purpose of this study was to determine if air thinning three dentin adhesives would affect bond strength to dentin. Ninety human molars were mounted in acrylic and the occlusal surfaces ground to expose a flat dentin surface. Thirty teeth were randomly assigned to one of the following dentin bonding agent/composite combinations: A) Universal Bond 3/TPH (Caulk), B) All-Bond 2/Bis-Fil-P (Bisco), and C) Scotchbond Multi-Purpose/Z-100 (3m). The primers were applied following the manufacturers' instructions. The adhesives were applied by two methods. A thin layer of adhesive was applied with a brush to 15 specimens in each group and light cured. Adhesive was brushed on to the remaining 15 teeth in the group, air thinned for 3 seconds, and then polymerized. The appropriate composite was applied in 2 mm increments and light cured utilizing a 5 mm-in-diameter split Teflon mold. Following 3 months of water storage, all groups were shear tested to failure on an Instron Universal Testing Machine. Bond strength was significantly higher in all groups when the dentin bonding agent was painted on without being air thinned. Scotchbond Multi-Purpose had significantly higher bond strength than All-Bond 2, which had significantly higher bond strength than Universal Bond 3.

  14. Adhesion of multimode adhesives to enamel and dentin after one year of water storage.

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    Vermelho, Paulo Moreira; Reis, André Figueiredo; Ambrosano, Glaucia Maria Bovi; Giannini, Marcelo

    2017-06-01

    This study aimed to evaluate the ultramorphological characteristics of tooth-resin interfaces and the bond strength (BS) of multimode adhesive systems to enamel and dentin. Multimode adhesives (Scotchbond Universal (SBU) and All-Bond Universal) were tested in both self-etch and etch-and-rinse modes and compared to control groups (Optibond FL and Clearfil SE Bond (CSB)). Adhesives were applied to human molars and composite blocks were incrementally built up. Teeth were sectioned to obtain specimens for microtensile BS and TEM analysis. Specimens were tested after storage for either 24 h or 1 year. SEM analyses were performed to classify the failure pattern of beam specimens after BS testing. Etching increased the enamel BS of multimode adhesives; however, BS decreased after storage for 1 year. No significant differences in dentin BS were noted between multimode and control in either evaluation period. Storage for 1 year only reduced the dentin BS for SBU in self-etch mode. TEM analysis identified hybridization and interaction zones in dentin and enamel for all adhesives. Silver impregnation was detected on dentin-resin interfaces after storage of specimens for 1 year only with the SBU and CSB. Storage for 1 year reduced enamel BS when adhesives are applied on etched surface; however, BS of multimode adhesives did not differ from those of the control group. In dentin, no significant difference was noted between the multimode and control group adhesives, regardless of etching mode. In general, multimode adhesives showed similar behavior when compared to traditional adhesive techniques. Multimode adhesives are one-step self-etching adhesives that can also be used after enamel/dentin phosphoric acid etching, but each product may work better in specific conditions.

  15. Shear bond strength of amalgam to dentin using different dentin adhesive systems

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    Farimah Sardari

    2012-01-01

    Full Text Available Background and Aims: The aim of this in vitro study was to assess the shear bond strength of amalgam to dentin using four dentin adhesive systems.Materials and Methods: One hundred human molars were selected. After enamel removal, a dentin cylinder with 3 mm thickness was prepared. Eighty specimens were resorted with amalgam and four dentin adhesive systems as follows (n=20: group 1, Scotch Bond Multi-Purpose; group 2, One Coat Bond; group 3, PQ1; and group 4, Panavia-F. In group 5, 20 specimens were resorted with amalgam and varnish as control group. The specimens were incubated at 37°C for 24 h. The shear bond strengths were then measured by using push out method. The data were analyzed by one-way ANOVA and post hoc Duncan's tests.Results: Mean values for bond strengths of test groups were as follows: group 1=21.03±8.9, group 2=23.47±9, group 3=13.16±8.8, group 4=20.07±8.9 and group 5=14.15±8.7 MPa±SD. One-way ANOVA showed the statistically significant difference between the bond strengths of five groups (P=0.001. Post hoc Duncan's test showed significant difference between groups 1and 3 (P=0.008, groups 1 and 5 (P=0.019, groups 2 and 5 (P=0.0008, groups 4 and 5 (P=0.042, and groups 3 and 4 (P=0.018.Conclusion: Results of this study showed that the bond strength of amalgam to dentin using One Coat Bond as dentin adhesive system was higher than that observed in other dentin adhesive systems.

  16. Effect of Nanofiller Addition to an Experimental Dentin Adhesive on Microtensile Bond Strength to Human Dentin

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    SH. Kasraei

    2009-06-01

    Full Text Available Objective: The purpose of the study was to evaluate the influence of adding nanofiller particles to a dentin bonding agent on resin-dentin bond strength.Materials and Methods: Fifty-four human intact premolar teeth were divided in to 6 groups of nine. The teeth were ground on occlusal surfaces and polished with 320 and then 600 grit silicon carbide papers. An experimental bonding system based on acetone/alcoholsolvent was provided with filler contents of 0.0, 0.5, 1.0, 2.5, 5.0, and 10.0 weight percent fumed silica nanofiller. After dentin surface etching, rinsing and blot drying, the experimentalbonding agents were applied to dentin surface. A composite resin was, then,bonded to the dentin on the bonding agent. The specimens were thermocycled for 500 cycles and sectioned in stick form. After two week of storage in distilled water, resin-dentin microtensile bond strength of the specimens was measured. Data were analyzed by one way ANOVA and DunnettT3 tests.Results: Bond strength to dentin was significantly affected by the filler level. Minimum and maximum resin-microtensile bond strength was in the experimental bonding agent with no filler (5.88 MPa and with filler level of 1.0 weight percent (15.15 MPa, respectively,and decreased with the increase of filler content down to 8.95 MPa for the filler level of 10.0 weight percent.Conclusion: Filler content seems to be one of the important factors influencing the bond strength of dental adhesives. Maximum dentin bond strength was obtained with 1% silanized nanofiller silica added to experimental adhesive system.

  17. Longevity of Self-etch Dentin Bonding Adhesives Compared to Etch-and-rinse Dentin Bonding Adhesives: A Systematic Review.

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    Masarwa, Nader; Mohamed, Ahmed; Abou-Rabii, Iyad; Abu Zaghlan, Rawan; Steier, Liviu

    2016-06-01

    A systematic review and meta-analysis were performed to compare longevity of Self-Etch Dentin Bonding Adhesives to Etch-and-Rinse Dentin Bonding Adhesives. The following databases were searched for PubMed, MEDLINE, Web of Science, CINAHL, the Cochrane Library complemented by a manual search of the Journal of Adhesive Dentistry. The MESH keywords used were: "etch and rinse," "total etch," "self-etch," "dentin bonding agent," "bond durability," and "bond degradation." Included were in-vitro experimental studies performed on human dental tissues of sound tooth structure origin. The examined Self-Etch Bonds were of two subtypes; Two Steps and One Step Self-Etch Bonds, while Etch-and-Rinse Bonds were of two subtypes; Two Steps and Three Steps. The included studies measured micro tensile bond strength (μTBs) to evaluate bond strength and possible longevity of both types of dental adhesives at different times. The selected studies depended on water storage as the aging technique. Statistical analysis was performed for outcome measurements compared at 24 h, 3 months, 6 months and 12 months of water storage. After 24 hours (p-value = 0.051), 3 months (p-value = 0.756), 6 months (p-value=0.267), 12 months (p-value=0.785) of water storage self-etch adhesives showed lower μTBs when compared to the etch-and-rinse adhesives, but the comparisons were statistically insignificant. In this study, longevity of Dentin Bonds was related to the measured μTBs. Although Etch-and-Rinse bonds showed higher values at all times, the meta-analysis found no difference in longevity of the two types of bonds at the examined aging times. Copyright © 2016 Elsevier Inc. All rights reserved.

  18. Bond strength and microleakage of current dentin adhesives.

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    Fortin, D; Swift, E J; Denehy, G E; Reinhardt, J W

    1994-07-01

    The purpose of this in vitro study was to evaluate shear bond strengths and microleakage of seven current-generation dentin adhesive systems. Standard box-type Class V cavity preparations were made at the cemento-enamel junction on the buccal surfaces of eighty extracted human molars. These preparations were restored using a microfill composite following application of either All-Bond 2 (Bisco), Clearfil Liner Bond (Kuraray), Gluma 2000 (Miles), Imperva Bond (Shofu), OptiBond (Kerr), Prisma Universal Bond 3 (Caulk), Scotchbond Multi-Purpose (3M), or Scotchbond Dual-Cure (3M) (control). Lingual dentin of these same teeth was exposed and polished to 600-grit. Adhesives were applied and composite was bonded to the dentin using a gelatin capsule technique. Specimens were thermocycled 500 times. Shear bond strengths were determined using a universal testing machine, and microleakage was evaluated using a standard silver nitrate staining technique. Clearfill Liner Bond and OptiBond, adhesive systems that include low-viscosity, low-modulus intermediate resins, had the highest shear bond strengths (13.3 +/- 2.3 MPa and 12.9 +/- 1.5 MPa, respectively). Along with Prisma Universal Bond 3, they also had the least microleakage at dentin margins of Class V restorations. No statistically significant correlation between shear bond strength and microleakage was observed in this study. Adhesive systems that include a low-viscosity intermediate resin produced the high bond strengths and low microleakage. Similarly, two materials with bond strengths in the intermediate range had significantly increased microleakage, and one material with a bond strength in the low end of the spectrum exhibited microleakage that was statistically greater. Thus, despite the lack of statistical correlation, there were observable trends.

  19. Tensile and bending fatigue of the adhesive interface to dentin.

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    Belli, Renan; Baratieri, Luiz Narciso; Braem, Marc; Petschelt, Anselm; Lohbauer, Ulrich

    2010-12-01

    The aim of this study was to evaluate the fatigue limits of the dentin-composite interfaces established either with an etch-and-rinse or an one-step self-etch adhesive systems under tensile and bending configurations. Flat specimens (1.2 mm×5 mm×35 mm) were prepared using a plexiglass mold where dentin sections from human third molars were bonded to a resin composite, exhibiting the interface centrally located. Syntac Classic and G-Bond were used as adhesives and applied according to the manufacturer's instructions. The fluorochrome Rhodamine B was added to the adhesives to allow for fractographic evaluation. Tensile strength was measured in an universal testing machine and the bending strength (n=15) in a Flex machine (Flex, University of Antwerp, Belgium), respectively. Tensile (TFL) and bending fatigue limits (BFL) (n=25) were determined under wet conditions for 10(4) cycles following a staircase approach. Interface morphology and fracture mechanisms were observed using light, confocal laser scanning and scanning electron microscopy. Statistical analysis was performed using three-way ANOVA (mod LSD test, pTensile and bending characteristic strengths at 63.2% failure probability for Syntac were 23.8 MPa and 71.5 MPa, and 24.7 MPa and 72.3 MPa for G-Bond, respectively. Regarding the applied methods, no significant differences were detected between adhesives. However, fatigue limits for G-Bond (TFL=5.9 MPa; BFL=36.2 MPa) were significantly reduced when compared to Syntac (TFL=12.6 MPa; BFL=49.7 MPa). Fracture modes of Syntac were generally of adhesive nature, between the adhesive resin and dentin, while G-Bond showed fracture planes involving the adhesive-dentin interface and the adhesive resin. Cyclic loading under tensile and bending configurations led to a significant strength degradation, with a more pronounced fatigue limit decrease for G-Bond. The greater decrease in fracture strength was observed in the tensile configuration. Copyright © 2010 Academy of

  20. Fracture toughness of dentin/resin-composite adhesive interfaces.

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    Tam, L E; Pilliar, R M

    1993-05-01

    The reliability and validity of tensile and shear bond strength determinations of dentin-bonded interfaces have been questioned. The fracture toughness value (KIC) reflects the ability of a material to resist crack initiation and unstable propagation. When applied to an adhesive interface, it should account for both interfacial bond strength and inherent defects at or near the interface, and should therefore be more appropriate for characterization of interface fracture resistance. This study introduced a fracture toughness test for the assessment of dentin/resin-composite bonded interfaces. The miniature short-rod specimen geometry was used for fracture toughness testing. Each specimen contained a tooth slice, sectioned from a bovine incisor, to form the bonded interface. The fracture toughness of an enamel-bonded interface was assessed in addition to the dentin-bonded interfaces. Tensile bond strength specimens were also prepared from the dentin surfaces of the cut bovine incisors. A minimum of ten specimens was fabricated for each group of materials tested. After the specimens were aged for 24 h in distilled water at 37 degrees C, the specimens were loaded to failure in an Instron universal testing machine. There were significant differences (p adhesives tested. Generally, both the fracture toughness and tensile bond strength measurements were highest for AllBond 2, intermediate for 3M MultiPurpose, and lowest for Scotchbond 2. Scanning electron microscopy of the fractured specimen halves confirmed that crack propagation occurred along the bond interface during the fracture toughness test. It was therefore concluded that the mini-short-rod fracture toughness test provided a valid method for characterization of the fracture resistance of the dentin-resin composite interface.

  1. Dentine bond strength and antimicrobial activity evaluation of adhesive systems.

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    André, Carolina Bosso; Gomes, Brenda Paula Figueiredo Almeida; Duque, Thais Mageste; Stipp, Rafael Nobrega; Chan, Daniel Chi Ngai; Ambrosano, Glaucia Maria Bovi; Giannini, Marcelo

    2015-04-01

    This study evaluated the dentine bond strength (BS) and the antibacterial activity (AA) of six adhesives against strict anaerobic and facultative bacteria. Three adhesives containing antibacterial components (Gluma 2Bond (glutaraldehyde)/G2B, Clearfil SE Protect (MDPB)/CSP and Peak Universal Bond (PUB)/chlorhexidine) and the same adhesive versions without antibacterial agents (Gluma Comfort Bond/GCB, Clearfil SE Bond/CSB and Peak LC Bond/PLB) were tested. The AA of adhesives and control groups was evaluated by direct contact method against four strict anaerobic and four facultative bacteria. After incubation, according to the appropriate periods of time for each microorganism, the time to kill microorganisms was measured. For BS, the adhesives were applied according to manufacturers' recommendations and teeth restored with composite. Teeth (n=10) were sectioned to obtain bonded beams specimens, which were tested after artificial saliva storage for one week and one year. BS data were analyzed using two-way ANOVA and Tukey test. Saliva storage for one year reduces the BS only for GCB. In general G2B and GCB required at least 24h for killing microorganisms. PUB and PLB killed only strict anaerobic microorganisms after 24h. For CSP the average time to eliminate the Streptococcus mutans and strict anaerobic oral pathogens was 30 min. CSB showed no AA against facultative bacteria, but had AA against some strict anaerobic microorganisms. Storage time had no effect on the BS for most of the adhesives. The time required to kill bacteria depended on the type of adhesive and never was less than 10 min. Most of the adhesives showed stable bond strength after one year and the Clearfil SE Protect may be a good alternative in restorative procedures performed on dentine, considering its adequate bond strength and better antibacterial activity. Copyright © 2015 Elsevier Ltd. All rights reserved.

  2. Post-bleaching application of an antioxidant on dentin bond strength of three dental adhesives

    Directory of Open Access Journals (Sweden)

    Maryam Khoroushi

    2012-01-01

    Conclusions: The findings suggest that bond strength of resin to bleached dentin may be affected with the adhesive system. Reduced SBS to bleached dentin can be amended by the use of SA as an antioxidizing agent. However, the amount of reversed bond strength subsequent to applying antioxidant might be related to the kind of dental adhesive.

  3. Microtensile bond strength of three simplified adhesive systems to caries-affected dentin.

    Science.gov (United States)

    Scholtanus, J D; Purwanta, Kenny; Dogan, Nilgun; Kleverlaan, Cees J; Feilzer, Albert J

    2010-08-01

    The purpose of the study was to determine the microtensile bond strength of three different simplified adhesive systems to caries-affected dentin. Fifteen extracted human molars with primary carious lesions were ground flat until dentin was exposed. Soft caries-infected dentin was excavated with the help of caries detector dye. On the remaining hard dentin, a standardized smear layer was created by polishing with 600-grit SiC paper. Teeth were divided into three groups and treated with one of the three tested adhesives: Adper Scotchbond 1 XT (3M ESPE), a 2-step etch-andrinse adhesive, Clearfil S3 Bond (Kuraray), a 1-step self-etching or all-in-one adhesive, and Clearfil SE Bond (Kuraray), a 2-step self-etching adhesive. Five-mm-thick composite buildups (Z-250, 3M ESPE) were built and light cured. After water storage for 24 h at 37ºC, the bonded specimens were sectioned into bars (1.0 x 1.0 mm; n = 20 to 30). Microtensile bond strength of normal dentin specimens and caries-affected dentin specimens was measured in a universal testing machine (crosshead speed = 1 mm/min). Data were analyzed using two-way ANOVA and Tukey's post-hoc test (p adhesives were found. Adper Scotchbond 1 XT and Clearfil S3 Bond showed significantly lower bond strength values to caries-affected dentin. For Clearfil SE Bond, bond strength values to normal and caries-affected dentin were not significantly different. All the tested simplified adhesives showed similar bond strength values to normal dentin. For the tested 2-step etch-and-rinse adhesive and the all-in-one adhesive, the bond strength values to caries-affected dentin were lower than to normal dentin.

  4. What's new in dentine bonding? Self-etch adhesives.

    Science.gov (United States)

    Burke, F J Trevor

    2004-12-01

    Bonding to dentine is an integral part of contemporary restorative dentistry, but early systems were not user-friendly. The introduction of new systems which have a reduced number of steps--the self-etch adhesives--could therefore be an advantage to clinicians, provided that they are as effective as previous adhesives. These new self-etch materials appear to form hybrid layers as did the previous generation of materials. However, there is a need for further clinical research on these new materials. Advantages of self-etch systems include, no need to etch and rinse, reduced post-operative sensitivity and low technique sensitivity. Disadvantages include, the inhibition of set of self- or dual-cure resin materials and the need to roughen untreated enamel surfaces prior to bonding.

  5. Failure in a composite resin-dentin adhesive bond

    Energy Technology Data Exchange (ETDEWEB)

    Rezgui, B. (Dept. de Genie Mecanique, Ecole Nationale d' Ingenieurs de Monastir, Monastir (Tunisia)); Abdennagi, H. (Dept. de Genie Mecanique, Ecole Nationale d' Ingenieurs de Monastir, Monastir (Tunisia)); Sahtout, S. (Dept. de Genie Mecanique, Ecole Nationale d' Ingenieurs de Monastir, Monastir (Tunisia) Dept. d' Odontologie, Faculte de Chirurgie Dentaire de Monastir (Tunisia)); Belkhir, M.S. (Dept. de Genie Mecanique, Ecole Nationale d' Ingenieurs de Monastir, Monastir (Tunisia) Dept. d' Odontologie, Faculte de Chirurgie Dentaire de Monastir (Tunisia))

    1993-11-01

    Composites are drawing more and more attention as preferred materials for teeth restoration. The success of teeth restoration has been generally limited by the Composite Resin-Dentin bond strength. A testing device has been developped to allow a satisfactory testing method for evaluating bonding strength in tension and shear, which led to reproducible results. A comparaison between different bond systems has shown no significant difference in the tensile and the shear strength as well as in the fracture behavior. Moreover, results showed difference between tensile and shear strength, when considering one same bond system. Failure mode examination turned out to be, either cohesive (composite rupture), or adhesive (interface rupture) or both (mixed rupture). (orig.).

  6. Effects of solvent evaporation time on immediate adhesive properties of universal adhesives to dentin.

    Science.gov (United States)

    Luque-Martinez, Issis V; Perdigão, Jorge; Muñoz, Miguel A; Sezinando, Ana; Reis, Alessandra; Loguercio, Alessandro D

    2014-10-01

    To evaluate the microtensile bond strengths (μTBS) and nanoleakage (NL) of three universal or multi-mode adhesives, applied with increasing solvent evaporation times. One-hundred and forty caries-free extracted third molars were divided into 20 groups for bond strength testing, according to three factors: (1) Adhesive - All-Bond Universal (ABU, Bisco, Inc.), Prime&Bond Elect (PBE, Dentsply), and Scotchbond Universal Adhesive (SBU, 3M ESPE); (2) Bonding strategy - self-etch (SE) or etch-and-rinse (ER); and (3) Adhesive solvent evaporation time - 5s, 15s, and 25s. Two extra groups were prepared with ABU because the respective manufacturer recommends a solvent evaporation time of 10s. After restorations were constructed, specimens were stored in water (37°C/24h). Resin-dentin beams (0.8mm(2)) were tested at 0.5mm/min (μTBS). For NL, forty extracted molars were randomly assigned to each of the 20 groups. Dentin disks were restored, immersed in ammoniacal silver nitrate, sectioned and processed for evaluation under a FESEM in backscattered mode. Data from μTBS were analyzed using two-way ANOVA (adhesive vs. drying time) for each strategy, and Tukey's test (α=0.05). NL data were computed with non-parametric tests (Kruskal-Wallis and Mann-Whitney tests, α=0.05). Increasing solvent evaporation time from 5s to 25s resulted in statistically higher mean μTBS for all adhesives when used in ER mode. Regarding NL, ER resulted in greater NL than SE for each of the evaporation times regardless of the adhesive used. A solvent evaporation time of 25s resulted in the lowest NL for SBU-ER. Residual water and/or solvent may compromise the performance of universal adhesives, which may be improved with extended evaporation times. Copyright © 2014 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

  7. Effect of Dentin Wetness on the Bond Strength of Universal Adhesives

    Directory of Open Access Journals (Sweden)

    An-Na Choi

    2017-10-01

    Full Text Available The effects of dentin wetness on the bond strength and adhesive interface morphology of universal adhesives have been investigated using micro-tensile bond strength (μTBS testing and confocal laser scanning microscopy (CLSM. Seventy-two human third molars were wet ground to expose flat dentin surfaces. They were divided into three groups according to the air-drying time of the dentin surfaces: 0 (without air drying, 5, and 10 s. The dentin surfaces were then treated with three universal adhesives: G-Premio Bond, Single Bond Universal, and All-Bond Universal in self-etch or etch-and-rinse mode. After composite build up, a μTBS test was performed. One additional tooth was prepared for each group by staining the adhesives with 0.01 wt % of Rhodamine B fluorescent dye for CLSM analysis. The data were analyzed statistically using ANOVA and Tukey’s post hoc tests (α = 0.05. Two-way ANOVA showed significant differences among the adhesive systems and dentin moisture conditions. An interaction effect was also observed (p < 0.05. One-way ANOVA showed that All-Bond Universal was the only material influenced by the wetness of the dentin surfaces. Wetness of the dentin surface is a factor influencing the micro-tensile bond strength of universal adhesives.

  8. Influence of previous acid etching on bond strength of universal adhesives to enamel and dentin.

    Science.gov (United States)

    Torres, Carlos Rocha Gomes; Zanatta, Rayssa Ferreira; Silva, Tatiane Josefa; Huhtala, Maria Filomena Rocha Lima; Borges, Alessandra Bühler

    2017-01-01

    The objective of this study was to evaluate the effect of acid pretreatment on the bond strength of composite resin bonded to enamel and dentin with 2 different universal self-etching adhesives. The null hypothesis was that the acid treatment performed prior to adhesive application would not significantly change the bond strength to enamel or dentin for either universal adhesive tested. A sample of 112 bovine incisors were selected and embedded in acrylic resin. Half were ground until a flat enamel surface was obtained, and the other half were polished until a 6 × 6-mm area of dentin was exposed, resulting into 2 groups (n = 56). The enamel and dentin groups were divided into 2 subgroups according to the adhesive system applied: Futurabond U or Scotchbond Universal. Each of these subgroups was divided into 2 additional subgroups (n = 14); 1 subgroup received phosphoric acid pretreatment, and 1 subgroup did not. The bond strength was assessed with a microtensile test. Data from enamel and dentin specimens were analyzed separately using 1-way analysis of variance. The acid pretreatment did not significantly change the bond strength of the adhesives tested, either to enamel (P = 0.4161) or to dentin (P = 0.4857). The acid etching pretreatment did not affect the bond strength to dentin and enamel when the tested universal multipurpose adhesive systems were used.

  9. Microtensile bond strength of three simplified adhesive systems to caries-affected dentin

    NARCIS (Netherlands)

    Scholtanus, J.D.; Purwanta, K.; Dogan, N.; Kleverlaan, C.J.; Feilzer, A.J.

    2010-01-01

    Purpose: The purpose of the study was to determine the microtensile bond strength of three different simplified adhesive systems to caries-affected dentin. Materials and Methods: Fifteen extracted human molars with primary carious lesions were ground flat until dentin was exposed. Soft

  10. Microtensile Bond Strength of Three Simplified Adhesive Systems to Caries-affected Dentin

    NARCIS (Netherlands)

    Scholtanus, Johannes; Purwanta, Kenny; Dogan, Nilgun; Kleverlaan, Cees J.; Feilzer, Albert J.

    Purpose: The purpose of the study was to determine the microtensile bond strength of three different simplified adhesive systems to caries-affected dentin. Materials and Methods: Fifteen extracted human molars with primary carious lesions were ground flat until dentin was exposed. Soft

  11. Solvent composition of one-step self-etch adhesives and dentine wettability.

    Science.gov (United States)

    Grégoire, Geneviève; Dabsie, Firas; Dieng-Sarr, Farimata; Akon, Bernadette; Sharrock, Patrick

    2011-01-01

    Our aim was to determine the wettability of dentine by four commercial self-etch adhesives and evaluate their spreading rate on the dentine surface. Any correlation with chemical composition was sought, particularly with the amount of solvent or HEMA present in the adhesive. The adhesives used were AdheSE One, Optibond All.In.One, Adper Easy Bond and XenoV. Chemical compositions were determined by proton nuclear magnetic resonance (NMR) spectroscopy of the adhesives dissolved in dimethylsulfoxide. Apparent contact angles for sessile drops of adhesives were measured on dentine slices as a function of time for up to 180s. The water contact angles were determined for fully polymerised adhesives. All adhesives were water-based with total solvent contents ranging from 27% to 73% for HEMA-free adhesives, and averaging 45% for HEMA containing adhesives. The contents in hydrophobic groups decreased as water contents increased. No differences were found in the adhesive contact angles after 180s even though the spreading rates were different for the products tested. Water contact angles differed significantly but were not correlated with HEMA or solvent presence. Manufacturers use different approaches to stabilise acid co-monomer ingredients in self-etch adhesives. Co-solvents, HEMA, or acrylamides without co-solvents are used to simultaneously etch and infiltrate dentine. A large proportion of water is necessary for decalcification action. Copyright © 2010 Elsevier Ltd. All rights reserved.

  12. Dentin bond strength and degree of conversion evaluation of experimental self-etch adhesive systems

    OpenAIRE

    Yazdi, Fatemeh-Maleknejad; Moosavi, Horieh; Atai, Mohammad; Zeynali, Mahsa

    2015-01-01

    Background The aim of this study was to investigate the effect of different concentrations of 10-methacryloyloxydecyl dihydrogen phosphate (10-MDP) monomer in one-step self-etch experimental adhesives on dentinal microshear bond strength (?SBS), their degree of conversion and bonded micro structure. Material and Methods Composite resin cylinders (Clearfil AP-X) were bonded on human sound molar dentinal surfaces by using five experimental one-step self-etching adhesives (1-SEAs) containing 0% ...

  13. The effect of dentine surface preparation and reduced application time of adhesive on bonding strength.

    Science.gov (United States)

    Saikaew, Pipop; Chowdhury, A F M Almas; Fukuyama, Mai; Kakuda, Shinichi; Carvalho, Ricardo M; Sano, Hidehiko

    2016-04-01

    This study evaluated the effects of surface preparation and the application time of adhesives on the resin-dentine bond strengths with universal adhesives. Sixty molars were cut to exposed mid-coronal dentine and divided into 12 groups (n=5) based on three factors; (1) adhesive: G-Premio Bond (GP, GC Corp., Tokyo, Japan), Clearfil Universal Bond (CU, Kuraray Noritake Dental Inc., Okayama, Japan) and Scotchbond Universal Adhesive (SB, 3M ESPE, St. Paul, MN, USA); (2) smear layer preparation: SiC paper ground dentine or bur-cut dentine; (3) application time: shortened time or as manufacturer's instruction. Fifteen resin-dentine sticks per group were processed for microtensile bond strength test (μTBS) according to non-trimming technique (1mm(2)) after storage in distilled water (37 °C) for 24h. Data were analyzed by three-way ANOVA and Dunnett T3 tests (α=0.05). Fractured surfaces were observed under scanning electron microscope (SEM). Another 12 teeth were prepared and cut into slices for SEM examination of bonded interfaces. μTBS were higher when bonded to SiC-ground dentine according to manufacturer's instruction. Bonding to bur-cut dentine resulted in significantly lower μTBS (padhesive resin interface. This was more pronounced when adhesives were bonded with a reduced application time and on bur cut dentine. The performance of universal adhesives can be compromised on bur cut dentine and when applied with a reduced application time. Copyright © 2016 Elsevier Ltd. All rights reserved.

  14. Does Shortened Application Time Affect Long-Term Bond Strength of Universal Adhesives to Dentin?

    Science.gov (United States)

    Saikaew, P; Matsumoto, M; Chowdhury, Afma; Carvalho, R M; Sano, H

    2018-04-09

    This study evaluated the effect of shortened application time on long-term bond strength with universal adhesives. Three universal adhesives were used: Clearfil Universal Bond (CU, Kuraray Noritake Dental Inc, Tokyo, Japan), Scotchbond Universal Adhesive (SB, 3M ESPE, St Paul, MN, USA) or G-Premio Bond (GP, GC Corp, Tokyo, Japan). Sixty molars were cut to expose midcoronal dentin and prepared with a regular diamond bur. Each adhesive was applied either according to the manufacturer's instruction or with shortened time. Specimens were stored in distilled water at 37°C for 24 hours and then cut into resin-dentin sticks. Microtensile bond strength (μTBS) was tested after either 24 hours or 1 year of water storage. Data were analyzed by the three-way ANOVA and Duncan tests ( α=0.05). Fracture modes were analyzed under a scanning electron microscope (SEM). One dentin stick per group was selected after fracture mode analysis and further observed using transmission electron microscopy (TEM). Six additional dentin discs were prepared and conditioned with each adhesive under the different application time to observe the adhesive-smear layer interaction by SEM. Shortened application time affected the μTBS ( puniversal adhesives to bur-cut dentin. The performance of universal adhesives can be compromised when applied using a shortened application time.

  15. Bonding effectiveness of self-etch adhesives to dentin after 24 h water storage.

    Science.gov (United States)

    Sarr, Mouhamed; Benoist, Fatou Leye; Bane, Khaly; Aidara, Adjaratou Wakha; Seck, Anta; Toure, Babacar

    2018-01-01

    This study evaluated the immediate bonding effectiveness of five self-etch adhesive systems bonded to dentin. The microtensile bond strength of five self-etch adhesives systems, including one two-step and four one-step self-etch adhesives to dentin, was measured. Human third molars had their superficial dentin surface exposed, after which a standardized smear layer was produced using a medium-grit diamond bur. The selected adhesives were applied according to their respective manufacturer's instructions for μTBS measurement after storage in water at 37°C for 24 h. The μTBS varied from 11.1 to 44.3 MPa; the highest bond strength was obtained with the two-step self-etch adhesive Clearfil SE Bond and the lowest with the one-step self-etch adhesive Adper Prompt L-Pop. Pretesting failures mainly occurring during sectioning with the slow-speed diamond saw were observed only with the one-step self-etch adhesive Adper Prompt L-Pop (4 out of 18). When bonded to dentin, the self-etch adhesives with simplified application procedures (one-step self-etch adhesives) still underperform as compared to the two-step self-etch adhesive Clearfil SE Bond.

  16. Effect of glutaraldehyde and ferric sulfate on shear bond strength of adhesives to primary dentin

    Directory of Open Access Journals (Sweden)

    Prabhakar A

    2008-12-01

    Full Text Available Aim: The present study was undertaken to evaluate the effect of alternative pulpotomy agents such as glutaraldehyde and ferric sulfate on the shear bond strength of self-etch adhesive systems to dentin of primary teeth. Materials and Methods: Eighty human primary molar teeth were sectioned in a mesiodistal direction and divided into experimental and control groups. Lingual dentin specimens in experimental groups were treated with glutaraldehyde and ferric sulfate. Buccal surfaces soaked in water served as control group. Each group was then divided into two groups based on the adhesive system used: Clearfil SE Bond and Adper Prompt L-Pop. A teflon mold was used to build the composite (Filtek Z-250 cylinders on the dentinal surface of all the specimens. Shear bond strength was tested for all the specimens with an Instron Universal Testing Machine. The failure mode analysis was performed with a Scanning Electron Microscope (SEM. Results: The results revealed that glutaraldehyde and ferric sulfate significantly reduced the shear bond strength of the tested adhesive systems to primary dentin. Clearfil SE Bond showed much higher shear bond strength than Adper Prompt L Pop to primary dentin. SEM analysis revealed a predominant cohesive failure mode for both adhesive systems. Conclusion: This study revealed that the pulpotomy medicaments glutaraldehyde and ferric sulfate adversely affected the bonding of self-etch adhesive systems to primary dentin.

  17. Long-term In Vitro Adhesion of Polyalkenoate-based Adhesives to Dentin.

    Science.gov (United States)

    Sezinando, Ana; Perdigão, Jorge; Ceballos, Laura

    2017-01-01

    To study the influence of a polyalkenoate copolymer (VCP) on the immediate (24 h) and 6-month dentin bonding stability of VCP-based adhesives, using microtensile bond strength (μTBS), nanoleakage (NL), and ultramorphological analyses (FE-SEM). Eighty-four caries-free molars were randomly assigned to seven adhesives: Clearfil SE Bond (CSE, Kuraray Noritake); Adper Single Bond Plus (SB, 3M ESPE); SB without VCP (SBnoVCP, 3M ESPE); Scotchbond Universal Adhesive applied as a etch-and-rinse adhesive (SBU_ER); SBU without VCP applied as an etch-and-rinse adhesive (SBUnoVCP_ER); SBU applied as a self-etch adhesive (SBU_SE, 3M ESPE); SBU without VCP applied as a self-etch adhesive (SBUnoVCP_SE, 3M ESPE). Half of the beams were tested after 24 h, and the other half was aged in water for 6 months prior to testing. For each tooth/evaluation time, two beams were randomly selected for NL analysis. Statistical analyses of µTBS results were performed using two-way ANOVA, Tukey's post-hoc tests, and Student's t-test for paired data (α = 0.05). Nanoleakage was statistically analyzed using the Kruskal-Wallis and Mann-Whitney tests, with Wilcoxon's test for paired data. For FE-SEM, four caries-free molars were assigned to each of the seven groups. Dentin disks were restored and cross sectioned into halves. One half was observed at 24 h, and the other at 6 months. The highest 6-month mean μTBS was obtained with SBU_SE/SBUnoVCP_SE and SBUnoVCP_ER. SBUnoVCP_SE resulted in greater silver deposition at 6 months. FE-SEM observations showed that CSE and SBU_SE specimens resulted in a submicron hybrid layer without signs of degradation at 6 months. VCP may contribute to the long-term bonding stability of VCP-based adhesives.

  18. Micromorphological characterization of adhesive interface of sound dentin and total-etch and self-etch adhesives.

    Science.gov (United States)

    Drobac, Milan; Stojanac, Igor; Ramić, Bojana; Premović, Milica; Petrović, Ljubomir

    2015-01-01

    The ultimate goal in restorative dentistry has always been to achieve strong and permanent bond between the dental tissues and filling materials. It is not easy to achieve this task because the bonding process is different for enamel and dentin-dentin is more humid and more organic than enamel. It is moisture and organic nature of dentin that make this hard tissue very complex to achieve adhesive bond. One of the first and most widely used tools for examining the adhesive bond between hard dental tissues and composite restorative materials is scanning electron microscopy. The aim of this study was scanning electron microscopy analyzes the interfacial micro morphology of total-etch and self-etch adhesives. Micro morphological characteristics of interface between total-etch adhesive (Prime & Bond NT) in combination with the corresponding composite (Ceram X Mono) were compared with those of self-etching adhesive (AdheSE One) in, combination with the corresponding composite (Tetric EvoCeram). The specimens were observed under 1000 x magnification of scanning electron microscopy (JEOL, JSM-6460 Low Vacuum). Measurement of the thickness of the hybrid layer of the examined com posite systems was performed with the software of the device used (NIH Image Analyser). Micromorphological analysis of interface showed that the hybrid layer in sound dentin was well formed, its average thickness being 2.68 microm, with a large number of resin tags and a large amount of lateral branches for specimens with a composite system Prime & Bond NT-Ceram X Mono. However, the specimens' with composite systems Adhese One-Tetric EvoCeram did not show the presence of hybrid layer and the resin tags were poorly represented. The results of this study suggest that total-etch adhesives bond better with sound dentin than self-etch adhesive.

  19. Effect of Fluoride-Releasing Adhesive Systems on the Mechanical Properties of Eroded Dentin.

    Science.gov (United States)

    Guedes, Ana Paula Albuquerque; Moda, Mariana Dias; Suzuki, Thaís Yumi Umeda; Godas, André Gustavo de Lima; Sundfeld, Renato Herman; Briso, André Luiz Fraga; Santos, Paulo Henrique dos

    2016-01-01

    The aim of the study was to evaluate the effect of erosive pH cycling with solutions that simulate dental erosion on Martens hardness (HMV) and elastic modulus (Eit) of dentin restored with fluoride-releasing adhesive systems. Twenty-seven bovine dentin slabs were restored with three adhesive systems: Adper Single Bond 2 total-etch adhesive system, One Up Bond F and Clearfil SE Protect fluoride-containing self-etching adhesive systems. The restorations were made with Filtek Z250. The HMV and Eit values at distances of 10, 30, 50 and 70 µm from the interface were evaluated using a dynamic ultra microhardness tester before and after immersion in deionized water, citric acid and hydrochloric acid (n=9). Data were submitted to repeated-measures ANOVA and Fisher's PLSD tests (=0.05). After erosive cycling, HMV values of dentin decreased in all groups. For dentin restored with Adper Single Bond 2, the lowest values were found closer to the hybrid layer, while for One Up Bond F and Clearfil SE Protect, the values remained unaltered at all distances. For dentin restored with fluoride-releasing adhesive systems, a decrease in Eit was found, but after 30 µm this difference was not significant. The acid substances were able to alter HMV and Eit of the underlying dentin. For fluoride-releasing adhesives, the greater the distance from bonded interface, the lower the Eit values. The fluoride in One Up Bond F and Clearfil SE Protect was able to protect the underlying dentin closer to the materials. In this way, the fluoride from adhesive systems could have some positive effect in the early stages of erosive lesions.

  20. Effect of Dentin Wetness on the Bond Strength of Universal Adhesives

    Science.gov (United States)

    Lee, Ji-Hye; Son, Sung-Ae; Jung, Kyoung-Hwa; Kwon, Yong Hoon

    2017-01-01

    The effects of dentin wetness on the bond strength and adhesive interface morphology of universal adhesives have been investigated using micro-tensile bond strength (μTBS) testing and confocal laser scanning microscopy (CLSM). Seventy-two human third molars were wet ground to expose flat dentin surfaces. They were divided into three groups according to the air-drying time of the dentin surfaces: 0 (without air drying), 5, and 10 s. The dentin surfaces were then treated with three universal adhesives: G-Premio Bond, Single Bond Universal, and All-Bond Universal in self-etch or etch-and-rinse mode. After composite build up, a μTBS test was performed. One additional tooth was prepared for each group by staining the adhesives with 0.01 wt % of Rhodamine B fluorescent dye for CLSM analysis. The data were analyzed statistically using ANOVA and Tukey’s post hoc tests (α = 0.05). Two-way ANOVA showed significant differences among the adhesive systems and dentin moisture conditions. An interaction effect was also observed (p adhesives. PMID:29068404

  1. Influence of laser etching on enamel and dentin bond strength of Silorane System Adhesive.

    Science.gov (United States)

    Ustunkol, Ildem; Yazici, A Ruya; Gorucu, Jale; Dayangac, Berrin

    2015-02-01

    The aim of this in vitro study was to evaluate the shear bond strength (SBS) of Silorane System Adhesive to enamel and dentin surfaces that had been etched with different procedures. Ninety freshly extracted human third molars were used for the study. After the teeth were embedded with buccal surfaces facing up, they were randomly divided into two groups. In group I, specimens were polished with a 600-grit silicon carbide (SiC) paper to obtain flat exposed enamel. In group II, the overlying enamel layer was removed and exposed dentin surfaces were polished with a 600-grit SiC paper. Then, the teeth in each group were randomly divided into three subgroups according to etching procedures: etched with erbium, chromium:yttrium-scandium-gallium-garnet laser (a), etched with 35% phosphoric acid (b), and non-etched (c, control). Silorane System Adhesive was used to bond silorane restorative to both enamel and dentin. After 24-h storage in distilled water at room temperature, a SBS test was performed using a universal testing machine at a crosshead speed of 1 mm/min. The data were analyzed using two-way ANOVA and Bonferroni tests (p enamel and dentin (p > 0.05). The SBS of self-etch adhesive to dentin was not statistically different from enamel (p > 0.05). Phosphoric acid treatment seems the most promising surface treatment for increasing the enamel and dentin bond strength of Silorane System Adhesive.

  2. Curing mode affects bond strength of adhesively luted composite CAD/CAM restorations to dentin.

    Science.gov (United States)

    Lührs, Anne-Katrin; Pongprueksa, Pong; De Munck, Jan; Geurtsen, Werner; Van Meerbeek, Bart

    2014-03-01

    To determine the effect of curing mode and restoration-surface pre-treatment on the micro-tensile bond strength (μTBS) to dentin. Sandblasted CAD/CAM composite blocks (LAVA Ultimate, 3M ESPE) were cemented to bur-cut dentin using either the etch & rinse composite cement Nexus 3 ('NX3', Kerr) with Optibond XTR ('XTR', Kerr), or the self-etch composite cement RelyX Ultimate ('RXU', 3M ESPE) with Scotchbond Universal ('SBU', 3M ESPE). All experimental groups included different 'curing modes' (light-curing of adhesive and cement ('LL'), light-curing of adhesive and auto-cure of cement ('LA'), co-cure of adhesive through light-curing of cement ('AL'), or complete auto-cure ('AA')) and different 'restoration-surface pre-treatments' of the composite block (NX3: either a silane primer (Kerr), or the XTR adhesive; RXU: either silane primer (RelyX Ceramic Primer, 3M ESPE) and SBU, or solely SBU). After water-storage (7 days, 37°C), the μTBS was measured. Additionally, the degree of conversion (DC) of both cements was measured after 10min and after 1 week, either auto-cured (21°C/37°C) or light-cured (directly/through 3-mm CAD/CAM composite). The linear mixed-effects model (α=0.05) revealed a significant influence of the factors 'curing mode' and 'composite cement', and a less significant effect of the factor 'restoration-surface pre-treatment'. Light-curing 'LL' revealed the highest μTBS, which decreased significantly for all other curing modes. For curing modes 'AA' and 'AL', the lowest μTBS and a high percentage of pre-testing failures were reported. Overall, DC increased with light-curing and incubation time. The curing mode is decisive for the bonding effectiveness of adhesively luted composite CAD/CAM restorations to dentin. Copyright © 2013 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

  3. Enzyme-catalyzed hydrolysis of dentin adhesives containing a new urethane-based trimethacrylate monomer

    Science.gov (United States)

    Park, Jong-Gu; Ye, Qiang; Topp, Elizabeth M.; Spencer, Paulette

    2009-01-01

    A new trimethacrylate monomer with urethane-linked groups, 1,1,1-tri-[4-(methacryloxyethylamino-carbonyloxy)-phenyl]ethane (MPE), was synthesized, characterized, and used as a co-monomer in dentin adhesives. Dentin adhesives containing 2-hydroxyethyl methacrylate (HEMA, 45% w/w) and 2,2-bis[4(2-hydroxy-3-methacryloyloxy-propyloxy)-phenyl] propane (BisGMA, 30% w/w) in addition to MPE (25% w/w) were formulated with H2O at 0 (MPE0), 8 (MPE8) and 16 wt % water (MPE16) to simulate the wet demineralized dentin matrix and compared with controls [HEMA/BisGMA, 45/55 w/w, at 0 (C0), 8 (C8) and 16 wt% water (C16)]. The new adhesive showed a degree of double bond conversion and mechanical properties comparable with control, with good penetration into the dentin surface and a uniform adhesive/dentin interface. On exposure to porcine liver esterase, the net cumulative methacrylic acid (MAA) release from the new adhesives was dramatically (P < 0.05) decreased relative to the control, suggesting that the new monomer improves esterase resistance. PMID:19582843

  4. PLGA nanoparticles as chlorhexidine-delivery carrier to resin-dentin adhesive interface.

    Science.gov (United States)

    Priyadarshini, Balasankar Meera; Mitali, Kakran; Lu, Thong Beng; Handral, Harish K; Dubey, Nileshkumar; Fawzy, Amr S

    2017-07-01

    To characterize and deliver fabricated CHX-loaded PLGA-nanoparticles inside micron-sized dentinal-tubules of demineralized dentin-substrates and resin-dentin interface. Nanoparticles fabricated by emulsion evaporation were assessed in-vitro by different techniques. Delivery of drug-loaded nanoparticles to demineralized dentin substrates, interaction with collagen matrix, and ex-vivo CHX-release profiles using extracted teeth connected to experimental setup simulating pulpal hydrostatic pressure were investigated. Furthermore, nanoparticles association/interaction with a commercial dentin-adhesive applied to demineralized dentin substrates were examined. The results showed that the formulated nanoparticles demonstrated attractive physicochemical properties, low cytotoxicity, potent antibacterial efficacy, and slow degradation and gradual CHX release profiles. Nanoparticles delivered efficiently inside dentinal-tubules structure to sufficient depth (>10μm) against the simulated upward pulpal hydrostatic-pressure, even after bonding-resins infiltration and were attached/retained on collagen-fibrils. These results verified the potential significance of this newly introduced drug-delivery therapeutic strategy for future clinical applications and promote for a new era of future dental research. This innovative drug-delivery strategy has proven to be a reliable method for delivering treatments that could be elaborated for other clinical applications in adhesive and restorative dentistry. Copyright © 2017 The Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

  5. Effect of adhesive resin cements on bond strength of ceramic core materials to dentin.

    Science.gov (United States)

    Gundogdu, M; Aladag, L I

    2018-03-01

    The aim of the present study was to evaluate the effects of self-etch and self-adhesive resin cements on the shear bond strength of ceramic core materials bonded to dentin. Extracted, caries-free, human central maxillary incisor teeth were selected, and the vestibule surfaces were cut flat to obtain dentin surfaces. Ceramic core materials (IPS e.max Press and Prettau Zirconia) were luted to the dentin surfaces using three self-etch adhesive systems (Duo-Link, Panavia F 2.0, and RelyX Ultimate Clicker) and two self-adhesive resin systems (RelyX U200 Automix and Maxcem Elite). A shear bond strength test was performed using a universal testing machine. Failure modes were observed under a stereomicroscope, and bonding interfaces between the adhesive resin cements and the teeth were evaluated with a scanning electron microscope. Data were analyzed with Student's t-test and one-way analysis of variance followed by Tukey's test (α = 0.05). The type of adhesive resin cement significantly affected the shear bond strengths of ceramic core materials bonded to dentin (P materials when the specimens were luted with self-adhesive resin cements (P materials.

  6. Paucity of Nanolayering in Resin-Dentin Interfaces of MDP-based Adhesives.

    Science.gov (United States)

    Tian, F; Zhou, L; Zhang, Z; Niu, L; Zhang, L; Chen, C; Zhou, J; Yang, H; Wang, X; Fu, B; Huang, C; Pashley, D H; Tay, F R

    2016-04-01

    Self-assembled nanolayering structures have been reported in resin-dentin interfaces created by adhesives that contain 10-methacryloyloxydecyl dihydrogen phosphate (10-MDP). These structures have been hypothesized to contribute to bond durability. The objective of the present study was to determine the extent of nanolayering in resin-dentin interfaces after application of commercialized 10-MDP-containing self-etch and universal adhesives to human dentin. Seven commercialized adhesives were examined: Adhese Universal (Ivoclar-Vivadent), All-Bond Universal (Bisco, Inc.), Clearfil SE Bond 2, Clearfil S3 Bond Plus, Clearfil Universal Bond (all from Kuraray Noritake Dental Inc.), G-Premio Bond (GC Corp.), and Scotchbond Universal (3M ESPE). Each adhesive was applied in the self-etch mode on midcoronal dentin according to the respective manufacturer's instructions. Bonded specimens (n = 6) were covered with flowable resin composite, processed for transmission electron microscopy, and examined at 30 random sites without staining. Thin-film glancing angle X-ray diffraction (XRD) was used to detect the characteristic peaks exhibited by nanolayering (n = 4). The control consisted of 15%wt, 10%wt, and 5%wt 10-MDP (DM Healthcare Products, Inc.) dissolved in a mixed solvent (ethanol and water weight ratio 9:8, with photoinitiators). Experimental primers were applied to dentin for 20 s, covered with hydrophobic resin layer, and examined in the same manner. Profuse nanolayering with highly ordered periodicity (~3.7 nm wide) was observed adjacent to partially dissolved apatite crystallites in dentin treated with the 15% 10-MDP primer. Three peaks in the 2θ range of 2.40° (3.68 nm), 4.78° (1.85 nm), and 7.18° (1.23 nm) were identified from thin-film XRD. Reduction in the extent of nanolayering was observed in the 10% and 5% 10-MDP experimental primer-dentin interface along with lower intensity XRD peaks. Nanolayering and characteristic XRD peaks were rarely observed in

  7. Shear bond strength of a new one-bottle dentin adhesive.

    Science.gov (United States)

    Swift, E J; Bayne, S C

    1997-08-01

    To test the shear bond strength of a new adhesive, 3M Single Bond, to dentin surfaces containing different degrees of moisture. Two commercially available one-bottle adhesives (Prime & Bond, One-Step) and a conventional three-step system (Scotchbond Multi-Purpose Plus) were included for comparison. 120 bovine teeth were embedded in acrylic and the labial surfaces were polished to 600 grit to create standardized dentin surfaces for testing. Resin composite was bonded to dentin using a gelatin capsule technique. Four adhesive systems were evaluated with three different degrees of surface moisture (moist, wet, and overwet). Shear bond strengths of adhesives to dentin were determined using a universal testing machine and analyzed by ANOVA and Tukey's post hoc tests. Single Bond had mean shear bond strengths of 19.2, 23.2 and 20.3 MPa to moist, wet, and overwet dentin, respectively. Bond strengths of the three-component system Scotchbond Multi-Purpose Plus ranged from 23.1 to 25.3 MPa, but were not significantly higher than the values for Single Bond. Prime & Bond had bond strengths similar to those of Single Bond, but One-Step had significantly lower bond strengths (P < 0.05) in the wet and overwet conditions.

  8. Effectiveness of immediate bonding of etch-and-rinse adhesives to simplified ethanol-saturated dentin

    Directory of Open Access Journals (Sweden)

    Leandro Afonso Guimarães

    2012-04-01

    Full Text Available This study examined the immediate bond strength of etch-and-rinse adhesives to demineralized dentin saturated with either water or absolute ethanol. The research hypothesis was that there would be no difference in bond strength to dentin between water or ethanol wet-bonding techniques. The medium dentin of 20 third molars was exposed (n = 5. The dentin surface was then acid-etched, left moist and randomly assigned to be saturated via either water wet-bonding (WBT or absolute ethanol wet-bonding (EBT. The specimens were then treated with one of the following etch-and-rinse adhesive systems: a 3-step, water-based system (Adper Scotchbond Multipurpose, or SBMP or a 2-step, ethanol/water-based system (Adper Single Bond 2, or SB. Resin composite build-ups were then incrementally constructed. After water storage for 24 h at 37°C, the tensile strength of the specimens was tested in a universal testing machine (0.5 mm/min. Data were analyzed by two-way ANOVA and Tukey's test (a = 5%. The failure modes were verified using a stereomicroscope (40'. For both adhesives, no significant difference in bond strength was observed between WBT and EBT (p > 0.05. The highest bond strength was observed for SB, regardless of the bonding technique (p < 0.05. No significant interaction between adhesives and bonding techniques was noticed (p = 0.597. There was a predominance of adhesive failures for all tested groups. The EBT and WBT displayed similar immediate bond strength means for both adhesives. The SB adhesive exhibited higher means for all conditions tested. Further investigations are needed to evaluate long-term bonding to dentin mediated by commercial etch-and-rinse adhesives using the EBT approach.

  9. Microtensile bond strength of contemporary adhesives to primary enamel and dentin.

    Science.gov (United States)

    Marquezan, Marcela; da Silveira, Bruno Lopes; Burnett, Luiz Henrique; Rodrigues, Célia Regina Martins Delgado; Kramer, Paulo Floriani

    2008-01-01

    The purpose of this study was to assess bond strength of three self-etching and two total-etch adhesive systems bonded to primary tooth enamel and dentin. Forty extracted primary human molars were selected and abraded in order to create flat buccal enamel and occlusal dentin surfaces. Teeth were assigned to one of the adhesive systems: Adper Scotch Bond Multi Purpose, Adper Single Bond 2, Adper Prompt L-Pop, Clearfil SE Bond and AdheSE. Immediately to adhesive application, a composite resin (Filtek Z250) block was built up. After 3 months of water storage, each sample was sequentially sectioned in order to obtain sticks with a square cross-sectional area of about 0.72 mm2. The specimens were fixed lengthways to a microtensile device and tested using a universal testing machine with a 50-N load cell at a crosshead speed of 0.5 mm/min. Microtensile bond strength values were recorded in MPa and compared by Analysis of Variance and the post hoc Tukey test (a = 0.05). In enamel, Clearfil SE Bond presented the highest values, followed by Adper Single Bond 2, AdheSE and Adper Scotch Bond Multi Purpose, without significant difference. The highest values in dentin were obtained with Adper Scotch Bond Multi Purpose and all other adhesives did not present significant different values from that, except Adper Prompt L-Pop that achieved the lowest bond strength in both substrates. Adper Scotch Bond Multi Purpose and Adper Single Bond 2 presented significantly lower values in enamel than in dentin although all other adhesives presented similar results in both substrates. contemporary adhesive systems present similar behaviors when bonded to primary teeth, with the exception of the one-step self-etching system; and self-etching systems can achieve bond strength values as good in enamel as in dentin of primary teeth.

  10. Effect of chlorhexidine on the adhesion of dental cements in dentin

    International Nuclear Information System (INIS)

    Carvajal Villalobos, Marco Vinicio; Torres Montoya, Jeimy; Vindas Ramirez, Cinthya Carolina

    2013-01-01

    The effect of pretreatment with 0.2% chlorhexidine was evaluated in the adhesion strength in the dentin shear of three self-adhesive cements after one week of storage. 48 healthy teeth (premolars and molars) extracted were used. The occlusal surface was removed, exposing the dentin. Self-adhesive cements RelyX U100 (3M ESPE), SpeedCEM (Ivoclar) and BisCem (Bisco) were placed in an Ultradent cylindrical abutment (2mm diameter) in dentin pretreated with 0.2% chlorhexidine for 60 seconds and in dentin without treatment for 40 seconds. Removed the cylinder all the samples were photocured an additional 40 seconds. After one week, the adhesion force was in eight groups on a universal test machine at 0.01 cm/min. The ANOVA tests were used to analyze the data. The use of chlorhexidine at 0.2% did not affect the average strength of adhesion in shear, while the type of cement if the affection [es

  11. Adhesive permeability affects coupling of resin cements that utilise self-etching primers to dentine.

    Science.gov (United States)

    Carvalho, R M; Pegoraro, T A; Tay, F R; Pegoraro, L F; Silva, N R F A; Pashley, D H

    2004-01-01

    To examine the effects of an experimental bonding technique that reduces the permeability of the adhesive layer on the coupling of resin cements to dentine. Extracted human third molars had their mid to deep dentin surface exposed flat by transversally sectioning the crowns. Resin composite overlays were constructed and cemented to the surfaces using either Panavia F (Kuraray) or Bistite II DC (Tokuyama) resin cements mediated by their respective one-step or two-step self-etch adhesives. Experimental groups were prepared in the same way, except that the additional layer of a low-viscosity bonding resin (LVBR, Scotchbond Multi-Purpose Plus, 3M ESPE) was placed on the bonded dentine surface before luting the overlays with the respective resin cements. The bonded assemblies were stored for 24 h in water at 37 degrees C and subsequently prepared for microtensile bond strength testing. Beams of approximately 0.8 mm(2) were tested in tension at 0.5 mm/min in a universal tester. Fractured surfaces were examined under scanning electron microscopy (SEM). Additional specimens were prepared and examined with TEM using a silver nitrate-staining technique. Two-way ANOVA showed significant interactions between materials and bonding protocols (p0.05). SEM observation of the fractured surfaces in Panavia F showed rosette-like features that were exclusive for specimens bonded according to manufacturer's directions. Such features corresponded well with the ultrastructure of the interfaces that showed more nanoleakage associated with the more permeable adhesive interface. The application of the additional layer of the LVBR reduced the amount of silver impregnation for both adhesives suggesting that reduced permeability of the adhesives resulted in improved coupling of the resin cements to dentin. Placement of an intermediate layer of a LVBR between the bonded dentine surface and the resin cements resulted in improved coupling of Panavia F to dentine.

  12. Smear layer-deproteinizing improves bonding of one-step self-etch adhesives to dentin.

    Science.gov (United States)

    Thanatvarakorn, Ornnicha; Prasansuttiporn, Taweesak; Thittaweerat, Suppason; Foxton, Richard M; Ichinose, Shizuko; Tagami, Junji; Hosaka, Keiichi; Nakajima, Masatoshi

    2018-03-01

    Smear layer deproteinizing was proved to reduce the organic phase of smear layer covered on dentin surface. It was shown to eliminate hybridized smear layer and nanoleakage expression in resin-dentin bonding interface of two-step self-etch adhesive. This study aimed to investigate those effects on various one-step self-etch adhesives. Four different one-step self-etch adhesives were used in this study; SE One (SE), Scotchbond™ Universal (SU), BeautiBond Multi (BB), and Bond Force (BF). Flat human dentin surfaces with standardized smear layer were prepared. Smear layer deproteinizing was carried out by the application of 50ppm hypochlorous acid (HOCl) on dentin surface for 15s followed by Accel ® (p-toluenesulfinic acid salt) for 5s prior to adhesive application. No surface pretreatment was used as control. Microtensile bond strength (μTBS) and nanoleakage under TEM observation were investigated. The data were analyzed by two-way ANOVA and Tukey's post-hoc test and t-test at the significant level of 0.05. Smear layer deproteinizing significantly improved μTBS of SE, SU, and BB (player observed in control groups of SE, BB, and BF, and reticular nanoleakage presented throughout the hybridized complex in control groups of BB and BF were eliminated upon the smear layer deproteinizing. Smear layer deproteinizing by HOCl and Accel ® application could enhance the quality of dentin for bonding to one-step self-etch adhesives, resulting in the improving μTBS, eliminating hybridized smear layer and preventing reticular nanoleakage formation in resin-dentin bonding interface. Copyright © 2018 The Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

  13. Shear bond strength of three adhesive systems to enamel and dentin of permanent teeth

    Directory of Open Access Journals (Sweden)

    Niloofar Shadman

    2012-01-01

    Full Text Available Background and Aims: The purpose of this experimental study was to investigate the shear bond strength of three new adhesive systems to enamel and dentin of permanent human teeth using three new etch and rinse and self-etch adhesive systems.Materials and Methods: Sixty intact caries-free third molars were selected and randomly divided into 6 groups. Flat buccal and lingual enamel and dentin surfaces were prepared and mounted in the acrylic resin perpendicular to the plan of the horizon. Adhesives used in this study were Tetric N-Bond, AdheSE and AdheSE-One F (Ivoclar/Vivadent, Schaan, Liechtenstein. The adhesives were applied on the surfaces and cured with quartz tungsten halogen curing unit (600 mW/cm2 intensity for 20 s. After attaching composite to the surfaces and thermocycling (500 cycles, 5-55ºC, shear bond strength was measured using a universal testing machine at a crosshead speed of 0.5 mm/min. The failure modes were examined under a stereomicroscope. The data were statistically analyzed using T-test, one-way ANOVA, Tukey and Fisher's exact tests.Results: In enamel, Tetric N-Bond (28.57±4.58 MPa and AdheSE (21.97±7.6 MPa had significantly higher bond strength than AdheSE-One F (7.16±2.09 MPa (P0.05.Conclusion: Shear bond strength to dentin in Tetric N-Bond (etch and rinse system( was higher than self-etch adhesives (AdheSE and AdheSE-One F. The bond strength to enamel and dentin in two-step self-etch (AdheSE was higher than one-step self-etch (AdheSE-One F.

  14. Long-term bond strength of adhesive systems applied to etched and deproteinized dentin

    Directory of Open Access Journals (Sweden)

    Ninoshka Uceda-Gómez

    2007-12-01

    Full Text Available The aim of this study was to evaluate the early and 12-month bond strength of two adhesive systems (Single Bond-SB and One Step-OS applied to demineralized dentin (WH and demineralized/NaOCl-treated dentin (H. Twenty flat dentin surfaces were exposed, etched, rinsed and slightly dried. For the H groups, a solution of 10% NaOCl was applied for 60 s, rinsed (15 s and slightly dried. The adhesives were applied according to the manufacturer's instructions and composite resin crowns were incrementally constructed. After 24 h (water-37ºC, the specimens was sectioned in order to obtain resin-dentin sticks (0.8 mm². The specimens were tested in microtensile (0.5 mm/min immediately (IM or after 12 months of water storage (12M. The data (MPa were subjected to ANOVA and Tukey's test (a=0.05. Only the main factors adhesive and time were significant (p=0.004 and p=0.003, respectively. SB (42.3±9.1 showed higher bond strengths than OS (33.6±11.6. The mean bond strength for IM-group (42.5±8.7 was statistically superior to 12M (33.3±11.8. The use of 10% NaOCl, after acid etching, did not improve the immediate and the long-term resin-dentin bond strength.

  15. Four-year water degradation of a total-etch and two self-etching adhesives bonded to dentin

    NARCIS (Netherlands)

    Abdalla, A.I.; Feilzer, A.J.

    2008-01-01

    Objectives: To evaluate effect of direct and indirect water storage on the microtensile dentin bond strength of one total-etch and two self-etching adhesives. Methods: The adhesive materials were: one total-etch adhesive; ‘Admira Bond’ and two selfetch adhesives; ‘Clearfil SE Bond’ and ‘Hybrid

  16. Enamel and dentin bond strengths of a new self-etch adhesive system.

    Science.gov (United States)

    Walter, Ricardo; Swift, Edward J; Boushell, Lee W; Braswell, Krista

    2011-12-01

    statement of problem:  Self-etch adhesives typically are mildly acidic and therefore less effective than etch-and-rinse adhesives for bonding to enamel.   The purpose of this study was to evaluate the enamel and dentin shear bond strengths of a new two-step self-etch adhesive system, OptiBond XTR (Kerr Corporation, Orange, CA, USA).   The labial surfaces of 80 bovine teeth were ground to create flat, 600-grit enamel or dentin surfaces. Composite was bonded to enamel or dentin using the new two-step self-etch system or a three-step etch-and-rinse (OptiBond FL, Kerr), two-step self-etch (Clearfil SE Bond, Kuraray America, Houston, TX, USA), or one-step self-etch adhesive (Xeno IV, Dentsply Caulk, Milford, DE, USA). Following storage in water for 24 hours, shear bond strengths were determined using a universal testing machine. The enamel and dentin data sets were subjected to separate analysis of variance and Tukey's tests. Scanning electron microscopy was used to evaluate the effects of each system on enamel.   Mean shear bond strengths to enamel ranged from 18.1 MPa for Xeno IV to 41.0 MPa for OptiBond FL. On dentin, the means ranged from 33.3 MPa for OptiBond FL to 47.1 MPa for Clearfil SE Bond. OptiBond XTR performed as well as Clearfil SE Bond on dentin and as well as OptiBond FL on enamel. Field emission scanning electron microscope revealed that OptiBond XTR produced an enamel etch pattern that was less defined than that of OptiBond FL (37.5% phosphoric acid) but more defined than that of Clearfil SE Bond or Xeno IV.   The new two-step self-etch adhesive system formed excellent bonds to enamel and dentin in vitro. OptiBond XTR, a new two-step self-etch adhesive system, is a promising material for bonding to enamel as well as to dentin. © 2011 Wiley Periodicals, Inc.

  17. Synthesis and evaluation of novel siloxane-methacrylate monomers used as dentin adhesives.

    Science.gov (United States)

    Ge, Xueping; Ye, Qiang; Song, Linyong; Misra, Anil; Spencer, Paulette

    2014-09-01

    The objectives of this study were to synthesize two new siloxane-methacrylate (SM) monomers for application in dentin adhesives and to investigate the influence of different functionality of the siloxane-containing monomers on the adhesive photopolymerization, water sorption, and mechanical properties. Two siloxane-methacrylate monomers (SM1 and SM2) with four and eight methacrylate groups were synthesized. Dentin adhesives containing BisGMA, HEMA and the siloxane-methacrylate monomers were photo-polymerized. The experimental adhesives were compared with the control adhesive (HEMA/BisGMA, 45/55, w/w) and characterized with regard to degree of conversion (DC), water miscibility of the liquid resin, water sorption and dynamic mechanical analysis (DMA). The experimental adhesives exhibited improved water miscibility as compared to the control. When cured in the presence of 12 wt% water to simulate the wet environment of the mouth, the SM-containing adhesives showed DC comparable to the control. The experimental adhesives showed higher rubbery modulus than the control under dry conditions. Under wet conditions, the mechanical properties of the formulations containing SM monomer with increased functionality were comparable with the control, even with more water sorption. The concentration and functionality of the newly synthesized siloxane-methacrylate monomers affected the water miscibility, water sorption and mechanical properties of the adhesives. The experimental adhesives show improved water compatibility compared with the control. The mechanical properties were enhanced with an increase of the functionality of the siloxane-containing monomers. The results provide critical structure/property relationships and important information for future development of durable, versatile siloxane-containing dentin adhesives. Published by Elsevier Ltd.

  18. Does hybridized dentin affect bond strength of self-adhesive resin cement?

    OpenAIRE

    Pamato, Saulo; do Valle, Acc?cio-Lins; de Andrade, Gustavo-Henrique-Barbosa; Vidotti, Hugo-Alberto; S?, Marcus-Vin?cius-Reis; Pereira, Jefferson-Ricardo

    2016-01-01

    Background Evaluate the influence of different hybridization bonding techniques of a self-adhesive resin cement. Material and Methods 30 human health molars were divided into six groups (n=10). The specimens received three longitudinal sections, allowing insertion of central cuts in PVC matrices. Each group received a different dentin pretreatment according to the manufacturer?s recommendations, except the control group (G1), as follows. G2 - a 3-step total-etch adhesive system (Optibond? FL,...

  19. Sealing ability and bond strength of four contemporary adhesives to enamel and to dentine.

    Science.gov (United States)

    Atash, R; Vanden Abbeele, A

    2005-12-01

    To compare the shear bond strength and microleakage of four adhesive systems to the enamel and dentine of primary bovine teeth. 120 bovine primary mandibular incisors were collected and stored in an aqueous 1% chloramine solution at room temperature for no longer than 3 months after extraction (80 for shear bond testing and 40 for microleakage evaluation). The adhesives tested were Clearfil SE bond (SE), Adper Prompt L Pop (LP), Xeno III (XE), and Prime and Bond NT (PB). For shear bond strength testing the specimens were wet ground to 600 grit SiC paper to expose a flat enamel or dentine surface. After bonding and restoration with Dyract AP (DAP), the teeth were subjected to shear stress using a universal testing machine. For microleakage evaluation, facial class V cavities were prepared half in enamel and half in cementum. All cavities were restored with DAP. After thermocycling and immersion in 2% methylene blue, the dye penetration was evaluated under a stereomicroscope. All data were analysed by Chi-square tests or Fisher's tests when adapted in order to determine the significant differences between groups. Results were considered as significant for p enamel 11.06 to 5.34, in decreasing order SE, LP, XE and PB and on dentine 10.47 to 4.74, in decreasing order SE, XE, LP and PB. Differences in bond strengths between the four systems on enamel and dentine were all statistically significant, excepted for XE vs LP (shear bond at dentine). No significant differences were recorded in the microleakage degree between the four adhesive systems on enamel and on dentine (p > 0.0.5). The highest shear bond strength was achieved by Clearfil SE bond and the lowest by Prime and Bond NT. There was no significant difference concerning the sealing ability of the four adhesive systems.

  20. Adhesive/Dentin Interface: The Weak Link in the Composite Restoration

    Science.gov (United States)

    Spencer, Paulette; Ye, Qiang; Park, Jonggu; Topp, Elizabeth M.; Misra, Anil; Marangos, Orestes; Wang, Yong; Bohaty, Brenda S.; Singh, Viraj; Sene, Fabio; Eslick, John; Camarda, Kyle; Katz, J. Lawrence

    2010-01-01

    Results from clinical studies suggest that more than half of the 166 million dental restorations that were placed in the United States in 2005 were replacements for failed restorations. This emphasis on replacement therapy is expected to grow as dentists use composite as opposed to dental amalgam to restore moderate to large posterior lesions. Composite restorations have higher failure rates, more recurrent caries, and increased frequency of replacement as compared to amalgam. Penetration of bacterial enzymes, oral fluids, and bacteria into the crevices between the tooth and composite undermines the restoration and leads to recurrent decay and premature failure. Under in vivo conditions the bond formed at the adhesive/dentin interface can be the first defense against these noxious, damaging substances. The intent of this article is to review structural aspects of the clinical substrate that impact bond formation at the adhesive/dentin interface; to examine physico-chemical factors that affect the integrity and durability of the adhesive/dentin interfacial bond; and to explore how these factors act synergistically with mechanical forces to undermine the composite restoration. The article will examine the various avenues that have been pursued to address these problems and it will explore how alterations in material chemistry could address the detrimental impact of physico-chemical stresses on the bond formed at the adhesive/dentin interface. PMID:20195761

  1. Hybridization quality and bond strength of adhesive systems according to interaction with dentin

    Science.gov (United States)

    Salvio, Luciana Andrea; Hipólito, Vinicius Di; Martins, Adriano Luis; de Goes, Mario Fernando

    2013-01-01

    Objective: To evaluate the hybridization quality and bond strength of adhesives to dentin. Materials and Methods: Ten human molars were ground to expose the dentin and then sectioned in four tooth-quarters. They were randomly divided into 5 groups according to the adhesive used: Two single-step self-etch adhesives – Adper Prompt (ADP) and Xeno III (XE), two two-step self-etching primer systems – Clearfil SE Bond (SE) and Adhe SE (ADSE), and one one-step etch-and-rinse system – Adper Single Bond (SB). Resin composite (Filtek Z250) crown buildups were made on the bonded surfaces and incrementally light-cured for 20 s. The restored tooth-quarters were stored in water at 37°C for 24 h and then sectioned into beams (0.8 mm2 in cross-section). Maximal microtensile bond strength (μ-TBS) was recorded (0.5 mm/min in crosshead speed). The results were submitted to one-way ANOVA and Tukey's test (α = 0.05). Thirty additional teeth were used to investigate the hybridization quality by SEM using silver methenamine or ammoniacal silver nitrate dyes. Results: SE reached significantly higher μ-TBS (P 0.05), and between SB and ADP (P > 0.05); ADSE and XE were significantly higher than SB and ADP (P adhesives with dentin. The hybridization quality was essential to improve the immediate μ-TBS to dentin. PMID:24926212

  2. Shear Bond Strengths and Morphological Evaluation of Filled and Unfilled Adhesive Interfaces to Enamel and Dentine

    Science.gov (United States)

    Mortazavi, Vajihesadat; Fathi, Mohammadhosein; Ataei, Ebrahim; Khodaeian, Niloufar; Askari, Navid

    2012-01-01

    In this laboratory study shear bond strengths of three filled and one unfilled adhesive systems to enamel and dentine were compared. Forty-eight extracted intact noncarious human mandibular molars were randomly assigned to two groups of 24 one for bonding to enamel and the other for bonding to dentine. Buccal and lingual surfaces of each tooth were randomly assigned for application of each one of filled (Prime & Bond NT (PBNT), Optibond Solo Plus (OBSP), and Clearfil SE Bond (CSEB)) and unfilled (Single Bond (SB)) adhesive systems (n = 12). A universal resin composite was placed into the translucent plastic cylinders (3 mm in diameter and 2 mm in length) and seated against the enamel and dentine surfaces and polymerized for 40 seconds. Shear bond strength was determined using a universal testing machine, and the results were statistically analyzed using two-way ANOVA, one-way ANOVA, t-test, and Tukey HSD post hoc test with a 5% level of significance.There were no statistically significant differences in bond strength between the adhesive systems in enamel, but CSEB and SB exhibited significantly higher and lower bond strength to dentine, respectively, than the other tested adhesive systems while there were no statistically significant differences between PBNT and OBSP. PMID:23209471

  3. Shear Bond Strengths and Morphological Evaluation of Filled and Unfilled Adhesive Interfaces to Enamel and Dentine

    Directory of Open Access Journals (Sweden)

    Vajihesadat Mortazavi

    2012-01-01

    Full Text Available In this laboratory study shear bond strengths of three filled and one unfilled adhesive systems to enamel and dentine were compared. Forty-eight extracted intact noncarious human mandibular molars were randomly assigned to two groups of 24 one for bonding to enamel and the other for bonding to dentine. Buccal and lingual surfaces of each tooth were randomly assigned for application of each one of filled (Prime & Bond NT (PBNT, Optibond Solo Plus (OBSP, and Clearfil SE Bond (CSEB and unfilled (Single Bond (SB adhesive systems (n=12. A universal resin composite was placed into the translucent plastic cylinders (3 mm in diameter and 2 mm in length and seated against the enamel and dentine surfaces and polymerized for 40 seconds. Shear bond strength was determined using a universal testing machine, and the results were statistically analyzed using two-way ANOVA, one-way ANOVA, t-test, and Tukey HSD post hoc test with a 5% level of significance.There were no statistically significant differences in bond strength between the adhesive systems in enamel, but CSEB and SB exhibited significantly higher and lower bond strength to dentine, respectively, than the other tested adhesive systems while there were no statistically significant differences between PBNT and OBSP.

  4. Amalgam shear bond strength to dentin using single-bottle primer/adhesive systems.

    Science.gov (United States)

    Cobb, D S; Denehy, G E; Vargas, M A

    1999-10-01

    To evaluate the in vitro shear bond strengths (SBS) of a spherical amalgam alloy (Tytin) to dentin using several single-bottle primer/adhesive systems both alone: Single Bond (SB), OptiBond Solo (Sol), Prime & Bond 2.1 (PB), One-Step (OS) and in combination with the manufacturer's supplemental amalgam bonding agent: Single Bond w/3M RelyX ARC (SBX) and Prime & Bond 2.1 w/Amalgam Bonding Accessory Kit (PBA). Two, three-component adhesive systems, Scotchbond Multi-Purpose (SBMP) and Scotchbond Multi-Purpose Plus w/light curing (S + V) and w/o light curing (S+) were used for comparison. One hundred eight extracted human third molars were mounted lengthwise in phenolic rings with acrylic resin. The proximal surfaces were ground to expose a flat dentin surface, then polished to 600 grit silicon carbide paper. The teeth were randomly assigned to 9 groups (n = 12), and dentin surfaces in each group were treated with an adhesive system according to the manufacturer's instructions, except for S + V specimens, where the adhesive was light cured for 10 s before placing the amalgam. Specimens were then secured in a split Teflon mold, having a 3 mm diameter opening and amalgam was triturated and condensed onto the treated dentin surfaces. Twenty minutes after condensation, the split mold was separated. Specimens were placed in distilled water for 24 hrs, then thermocycled (300 cycles, between 5 degrees C and 55 degrees C, with 12 s dwell time). All specimens were stored in 37 degrees C distilled water for 7 days, prior to shear strength testing using a Zwick Universal Testing Machine at a cross-head speed of 0.5 mm/min. The highest to the lowest mean dentin shear bond strength values (MPa) for the adhesive systems tested were: S + V (10.3 +/- 2.3), SBX (10.2 +/- 3.5), PBA, (6.4 +/- 3.6), SOL (5.8 +/- 2.5), SBMP (5.7 +/- 1.8), S+ (4.8 +/- 2.3), PB (2.7 +/- 2.6), SB (2.7 +/- 1.1) and OS (2.5 +/- 1.8). One-way ANOVA and Duncan's Multiple Range Test indicated significant

  5. Halloysite nanotube incorporation into adhesive systems—effect on bond strength to human dentin.

    Science.gov (United States)

    Alkatheeri, Mohammed S; Palasuk, Jadesada; Eckert, George J; Platt, Jeffrey A; Bottino, Marco C

    2015-11-01

    This study aimed to evaluate the effect of Halloysite® aluminosilicate clay nanotube (HNT) incorporation into a two-step etch-and-rinse (ER) and a one-step self-etch (SE) adhesive on human dentin shear bond strength (SBS). Ten groups (n = 12) were prepared according to the adhesive system (i.e., ER or SE) and amount of HNT incorporated (5-20%, w/v), as follows: commercial control (i.e., the adhesive was used as purchased, 0% HNT); experimental control (i.e., the adhesive was processed through mixing/stirring and sonication similarly to the HNT-incorporated experimental groups, but without HNT); and 5, 10, and 20% HNT. SBS testing was performed after 24 h of storage in deionized water at 37 °C. Failure modes were examined using a stereomicroscope (×40). Scanning electron microscopy (SEM) of the resin-dentin interface of selected specimens was carried out. Two-way ANOVA revealed that incorporation of HNT up to 20% (w/v) in ER and up to 10% (w/v) in SE demonstrated an increased SBS compared to their experimental controls. Compared to the commercial control, SBS of HNT-modified dentin adhesives was not significantly different for ER adhesives (p > 0.05) but was significantly higher with 5% HNT in the SE adhesive (p < 0.05). Failure modes were predominantly adhesive and mixed failures. SEM micrographs of resin-dentin interfaces for ER-commercial control and ER-10% showed a similar morphology. A thicker adhesive layer and the presence of agglomerated HNT on the resin tags were seen in ER-10%. An increased number of short resin tags in SE-5% compared with SE-commercial control were observed. HNT addition up to 20% in ER and up to 10 % in SE showed increased SBS to dentin compared with the experimental control. HNT can be used not only to reinforce adhesive resins but also hold potential for the development of bioactive adhesives by the encapsulation of matrix metalloproteinase (MMP) inhibitors or anticariogenic agents.

  6. [The durability of three self-etch adhesives bonded to dentin].

    Science.gov (United States)

    Tian, Fu-Cong; Wang, Xiao-Yan; Gao, Xue-Jun

    2013-04-01

    To investigate the durability of self-etch adhesives bonded to dentin in vitro. Forty-two extracted human molars were selected and occlusal dentin surfaces were exposed. The teeth were randomly distributed into three groups based on adhesives applied. The one-step self-etch adhesive B(Adper Prompt) and C(G-Bond) and two-step self-etch adhesive A (Clearfil SE bond) were used. After application of the adhesives to the dentin surfaces, composite crowns were built up, after 24 h water storage, the teeth were sectioned longitudinally into sticks (1.0 mm×1.0 mm bonding area) for microtensile testing or slabs (1 mm thick) for scanning electron microscopec (SEM) observation. Bonding strength (mTBS) and nano-leakage were evaluated immediately after cutting or after 6 months in water. The mTBS was analyzed using one-way ANOVA (SPSS 13.0). The nanoleakage was observed by SEM with a backscattered electron detector. Both adhesives and water storage time affected the mTBS. All adhesives showed decreased bond strength after six-month water aging [A dropped from (40.60 ± 5.76) MPa to (36.04 ± 3.15) MPa; B dropped from (19.06 ± 1.50) MPa to (11.19 ± 1.97) MPa; C dropped from (17.75 ± 1.10) MPa to (9.14 ± 1.15) MPa] (P adhesives tested were probably influenced by water aging, however, the two-step adhesive showed better durability than the one-step adhesives.

  7. Effects of Different Radiation Doses on the Bond Strengths of Two Different Adhesive Systems to Enamel and Dentin.

    Science.gov (United States)

    da Cunha, Sandra Ribeiro de Barros; Ramos, Pedro Augusto Minorin Mendes; Haddad, Cecília Maria Kalil; da Silva, João Luis Fernandes; Fregnani, Eduardo Rodrigues; Aranha, Ana Cecília Corrêa

    2016-01-01

    To evaluate the effects of three different radiation doses on the bond strengths of two different adhesive systems to enamel and dentin. Eighty human third molars were randomly divided into four groups (n = 20) according to the radiation dose (control/no radiation, 20 Gy, 40 Gy, and 70 Gy). The teeth were sagittally sectioned into three slices: one mesial and one distal section containing enamel and one middle section containing dentin. The sections were then placed in the enamel and dentin groups, which were further divided into two subgroups (n = 10) according to the adhesive used. Three restorations were performed in each tooth (one per section) using Adper Single Bond 2 (3M ESPE) or Universal Single Bond (3M ESPE) adhesive system and Filtek Z350 XT (3M ESPE) resin composite and subjected to the microshear bond test. Data were analyzed using a two-way ANOVA followed by Tukey's test. Failure modes were examined under a stereoscopic loupe. Radiotherapy did not affect the bond strengths of the adhesives to either enamel or dentin. In dentin, the Universal Single Bond adhesive system showed higher bond strength values when compared with the Adper Single Bond adhesive system. More adhesive failures were observed in the enamel for all radiation doses and adhesives. Radiotherapy did not influence the bond strength to enamel or dentin, irrespective of the adhesive or radiation dose used.

  8. Effect of Adhesive Cementation Strategies on the Bonding of Y-TZP to Human Dentin.

    Science.gov (United States)

    Alves, Mll; Campos, F; Bergoli, C D; Bottino, M A; Özcan, M; Souza, Roa

    2016-01-01

    This study evaluated the effects of different adhesive strategies on the adhesion of zirconia to dentin using conventional and self-adhesive cements and their corresponding adhesive resins. The occlusal parts of human molars (N=80) were sectioned, exposing the dentin. The teeth and zirconia cylinders (N=80) (diameter=3.4 mm; height=4 mm) were randomly divided into eight groups according to the factors "surface conditioning" and "cement type" (n=10 per group). One conventional cement (CC: RelyX ARC, 3M ESPE) and one self-adhesive cement (SA: RelyX U200, 3M ESPE) and their corresponding adhesive resin (for CC, Adper Single Bond Plus; for SA, Scotchbond Universal Adhesive-SU) were applied on dentin. Zirconia specimens were conditioned either using chairside (CJ: CoJet, 30 μm, 2.5 bar, four seconds), laboratory silica coating (RC: Rocatec, 110 μm, 2.5 bar, four seconds), or universal primer (Single Bond Universal-UP). Nonconditioned groups for both cements acted as the control (C). Specimens were stored in water (37°C, 30 days) and subjected to shear bond strength (SBS) testing (1 mm/min). Data (MPa) were analyzed using two-way analysis of variance and a Tukey test (α=0.05). While surface conditioning significantly affected the SBS values (p=0.0001) (Cadhesive. Air-abrasion and the use of the universal primer improved the bond strength of zirconia to dentin compared to the control group, regardless of the type of resin cement used.

  9. Bonding of universal adhesives to dentine--Old wine in new bottles?

    Science.gov (United States)

    Chen, C; Niu, L-N; Xie, H; Zhang, Z-Y; Zhou, L-Q; Jiao, K; Chen, J-H; Pashley, D H; Tay, F R

    2015-05-01

    Multi-mode universal adhesives offer clinicians the choice of using the etch-and-rinse technique, selective enamel etch technique or self-etch technique to bond to tooth substrates. The present study examined the short-term in vitro performance of five universal adhesives bonded to human coronal dentine. Two hundred non-carious human third molars were assigned to five groups based on the type of the universal adhesives (Prime&Bond Elect, Scotchbond Universal, All-Bond Universal, Clearfil Universal Bond and Futurabond U). Two bonding modes (etch-and-rinse and self-etch) were employed for each adhesive group. Bonded specimens were stored in deionized water for 24h or underwent a 10,000-cycle thermocycling ageing process prior to testing (N=10). Microtensile bond testing (μTBS), transmission electron microscopy (TEM) of resin-dentine interfaces in non-thermocycled specimens and scanning electron microscopy (SEM) of tracer-infused water-rich zones within hybrid layers of thermocycled specimens were performed. Both adhesive type and testing condition (with/without thermocycling) have significant influences on μTBS. The use of each adhesive in either the etch-and-rinse or self-etch application mode did not result in significantly different μTBS to dentine. Hybrid layers created by these adhesives in the etch-and-rinse bonding mode and self-etch bonding mode were ∼5μm and ≤0.5μm thick respectively. Tracer-infused regions could be identified within the resin-dentine interface from all the specimens prepared. The increase in versatility of universal adhesives is not accompanied by technological advances for overcoming the challenges associated with previous generations of adhesives. Therapeutic adhesives with bio-protective and bio-promoting effects are still lacking in commercialized adhesives. Universal adhesives represent manufacturers' attempt to introduce versatility in product design via adaptation of a single-bottle self-etch adhesive for other application

  10. The influence of salivary contamination on shear bond strength of dentin adhesive systems.

    Science.gov (United States)

    Park, Jeong-won; Lee, Kyung Chae

    2004-01-01

    This study evaluated the influence of salivary contamination during dentin bonding procedures on shear bond strength and investigated the effect of contaminant-removing treatments on the recovery of bond strength for two dentin bonding agents. One hundred and ten human molars were embedded in cylindrical molds with self-curing acrylic resin. The occlusal dentin surface was exposed by wet grinding with #800 silicon carbide abrasive paper. The teeth were divided into five groups for One-step (OS) (BISCO, Inc) and six groups for Clearfil SE Bond (SE) (Kuraray Co, Ltd, Osaka, Japan). For One-step, the grinding surface was treated with 32% phosphoric acid; BAC (BISCO Inc) and divided into five groups: OS control group (uncontaminated), OS I (salivary contamination, blot dried), OS II (salivary contamination, completely dried), OS III (salivary contamination, wash and blot dried) and OS IV (salivary contamination, re-etching for 10 seconds, wash and blot dried). For SE bond, the following surface treatments were done: SE control group (primer applied to the fresh dentin surface), SE I (after salivary contamination, primer applied), SE II (primer, salivary contamination, dried), SE III (primer, salivary contamination, wash and dried), SE IV (after procedure of SE II, re-application of primer) and SE V (after procedure of SE III, re-application of primer). Each bonding agent was applied and light cured for 10 seconds. Clearfil AP-X (Kuraray Co, Ltd) composite was packed into the Ultradent mount jig mold and light cured for 40 seconds. The bonded specimens were stored for 24 hours in a 37 degrees C waterbath. The shear bond strengths were measured using an Instron testing machine (Model 4202, Instron Corp). The data for each group were subjected to one-way ANOVA followed by the Newman-Keuls test to make comparisons among the groups. The results were as follows: In the One-step groups, the OS II group showed statistically significant lower shear bond strength than the OS

  11. Mini-interfacial Fracture Toughness of a Multimode Adhesive Bonded to Plasma-treated Dentin.

    Science.gov (United States)

    Ayres, Ana Paula Almeida; Pongprueksa, Pong; De Munck, Jan; Gré, Cristina Parise; Nascimento, Fábio Dupart; Giannini, Marcelo; Van Meerbeek, Bart

    2017-01-01

    To investigate the bonding efficacy of a multimode adhesive to plasma-treated and -untreated (control) dentin using a mini-interfacial fracture toughness (mini-iFT) test. Twenty human molars were used in a split-tooth design (n = 10). The adhesive Scotchbond Universal (SBU; 3M ESPE) was applied in etch-and-rinse (E&R) and self-etch (SE) modes. Mid-coronal dentin was exposed and covered with a standardized smear layer ground to 320 grit. One half of each dentin surface received 15 s of non-thermal atmospheric plasma (NTAP), while the other half was covered with a metallic barrier and kept untreated. Following the E&R mode, dentin was plasma treated immediately after phosphoric acid etching. SBU and a resin-based composite were applied to dentin following the manufacturer's instructions. Six mini-iFT specimens were prepared per tooth (1.5 x 2.0 x 16 to 18 mm), and a single notch was prepared at the adhesive-dentin interface using a 150-μm diamond blade under water cooling. Half of the mini-iFT specimens were immediately loaded until failure in a 4-point bending test, while the other half were first stored in distilled water for 6 months. After testing, the exact dimensions of the notch were measured with a measuring optical microscope, from which ΚIc was determined. Three-way ANOVA revealed higher mini-iFT for SBU applied in E&R than SE mode for both storage times, irrespective of NTAP treatment. Overall, mini-iFT did not decrease for any of the experimental groups upon 6-month aging, while plasma treatment did not show a direct beneficial effect on mini-iFT of SBU applied in either E&R or SE mode.

  12. Influence of degradation conditions on dentin bonding durability of three universal adhesives.

    Science.gov (United States)

    Sai, Keiichi; Shimamura, Yutaka; Takamizawa, Toshiki; Tsujimoto, Akimasa; Imai, Arisa; Endo, Hajime; Barkmeier, Wayne W; Latta, Mark A; Miyazaki, Masashi

    2016-11-01

    This study aims to determine dentin bonding durability of universal adhesives using shear bond strength (SBS) tests under various degradation conditions. G-Premio Bond (GP, GC), Scotchbond Universal (SU, 3M ESPE) and All Bond Universal (AB, Bisco) were compared with conventional two-step self-etch adhesive Clearfil SE Bond (SE, Kuraray Noritake Dental). Bonded specimens were divided into three groups of ten, and SBSs with bovine dentin were determined after the following treatments: 1) Storage in distilled water at 37°C for 24h followed by 3000, 10,000, 20,000 or 30,000 thermal cycles (TC group), 2) Storage in distilled water at 37°C for 3 months, 6 months or 1year (water storage, WS group) and 3) Storage in distilled water at 37°C for 24h (control). SE bonded specimens showed significantly higher SBSs than universal adhesives, regardless of TC or storage periods, although AB specimens showed significantly increased SBSs after 30,000 thermal cycles. In comparisons of universal adhesives under control and degradation conditions, SBS was only reduced in SU after 1year of WS. Following exposure of various adhesive systems to degradation conditions of thermal cycling and long term storage, SBS values of adhesive systems varied primarily with degradation period. Although universal adhesives have lower SBSs than the two-step self-etch adhesive SE, the present data indicate that the dentin bonding durability of universal adhesives in self-etch mode is sufficient for clinical use. Copyright © 2016 Elsevier Ltd. All rights reserved.

  13. Dentin bond strength and nanoleakage of the adhesive interface after intracoronal bleaching.

    Science.gov (United States)

    Cavalli, Vanessa; Sebold, Maicon; Shinohara, Mirela Sanae; Pereira, Patrícia Nóbrega Rodrigues; Giannini, Marcelo

    2018-04-01

    This study evaluated dentin bond strength (BS) and nanoleakage of non- and pre-etched dentin immediately (T 0 ,), 7 days (T 7 ), and 14 days (T 14 ) after bleaching. Bovine incisors (150) were selected and half of them submitted to intrapulpal dentin etching (e). Non- and pre-etched dentin were subjected to the following (n = 15): no bleaching/control (C); 35% carbamide peroxide (CP); 35% hydrogen peroxide (35% HP); 25% hydrogen peroxide (25% HP); and sodium perborate (SP). Bleaching agents were applied to the pulp chamber four times within a 72-h interval. Afterwards, pulp chamber dentin was prepared for the BS test at different evaluation times (n = 5): T 0 , T 7 , and T 14 . Composite blocks were built on pulp chamber and sectioned in slices. Slices were reduced to an hour-glass shape with a cross-sectional area of 0.8 mm 2 and submitted to microtensile BS test. Two additional specimens for each group were prepared for nanoleakage evaluation by transmission electron microscopy (TEM). Results were analyzed by ANOVA (two-way) and Dunnett's test (p bleaching for both sound and pre-etched dentin (p bleached dentin increased for all groups, whereas the pre-etched SPe group presented BS similar to the Ce. Nanoleakage within the hybrid layer was perceptible immediately after bleaching, although a decrease in nanoleakage was observed for all groups at T 14 . Adhesive restorations should be performed 7-14 days after bleaching, according to the bleaching agent used. Intracoronal bleaching should be performed preferably with sodium perborate if previous dentin etching is applied. © 2018 Wiley Periodicals, Inc.

  14. Fracture toughness versus micro-tensile bond strength testing of adhesive-dentin interfaces.

    Science.gov (United States)

    De Munck, Jan; Luehrs, Anne-Katrin; Poitevin, André; Van Ende, Annelies; Van Meerbeek, Bart

    2013-06-01

    To assess interfacial fracture toughness of different adhesive approaches and compare to a standard micro-tensile bond-strength (μTBS) test. Chevron-notched beam fracture toughness (CNB) was measured following a modified ISO 24370 standard. Composite bars with dimensions of 3.0×4.0×25 mm were prepared, with the adhesive-dentin interface in the middle. At the adhesive-dentin interface, a chevron notch was prepared using a 0.15 mm thin diamond blade mounted in a water-cooled diamond saw. Each specimen was loaded until failure in a 4-point bend test setup and the fracture toughness was calculated according to the ISO specifications. Similarly, adhesive-dentin micro-specimens (1.0×1.0×8-10 mm) were stressed in tensile until failure to determine the μTBS. A positive correlation (r(2)=0.64) was observed between CNB and μTBS, which however was only nearly statistically significant, mainly due to the dissimilar outcome of Scotchbond Universal (3M ESPE). While few μTBS specimens failed at the adhesive-dentin interface, almost all CNB specimens failed interfacially at the notch tip. Weibull moduli for interfacial fracture toughness were much higher than for μTBS (3.8-11.5 versus 2.7-4.8, respectively), especially relevant with regard to early failures. Although the ranking of the adhesives on their bonding effectiveness tested using CNB and μTBS corresponded well, the outcome of CNB appeared more reliable and less variable. Fracture toughness measurement is however more laborious and requires specific equipment. The μTBS nevertheless appeared to remain a valid method to assess bonding effectiveness in a versatile way. Copyright © 2013 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

  15. Effect of adhesive system and application strategy on reduction of dentin permeability

    Directory of Open Access Journals (Sweden)

    Adriana Oliveira Carvalho

    2012-10-01

    Full Text Available This study evaluated the effect of adhesive systems and application strategies on dentin hydraulic conductance (HC. The buccal enamel was removed from bovine incisors to simulate laminate cavity preparations. After removing the roots and the coronal pulp, the buccal dentin was treated with EDTA solution (0.5 M for 5 minutes, rinsed, ultrasonicated for 12 minutes and connected to a permeability device. HC of the specimens was measured at 10 psi (n = 5. Permeability was measured before and after bonding procedures using G-Bond (GB, Clearfil Tri-S Bond (CTS, Hybrid Coat (HY, Bond Force (BF, Adper Easy Bond (AEB Silorane (SI, Clearfil SE Bond (CSE and Adper Scotchbond Multi-Purpose (SMP adhesives systems, which were applied following three strategies: 1 according to the manufacturers' instructions; 2 two coats of all-in-one self-etching adhesives (GB, CTS, HY, BF, AEB or priming step plus two coats of bonding resin for the other systems (SI, CSE and SMP; and 3 a thin layer of a flowable composite applied over one coat of all-in-one self-etching adhesives or primed surface for SI, CSE and SMP adhesives. No significant difference was observed among the application modes concerning their ability to reduce HC. None of the adhesives showed complete sealing (100% of the bovine tooth dentin. SI exhibited lower HC than SMP, however, they were not significantly different from the other systems. The results suggest that all systems tested result in an HC reduction of more than 90%. The wet bonding technique seemed to be more sensitive for dentin sealing.

  16. Effect of dentin biomodifiers on the immediate and long-term bond strengths of a simplified etch and rinse adhesive to dentin.

    Science.gov (United States)

    Singh, Payal; Nagpal, Rajni; Singh, Udai Pratap

    2017-08-01

    This in vitro study evaluated the effect of dentin biomodifiers on the immediate and long-term bond strengths of a simplified etch and rinse adhesive to dentin. Flat coronal dentin surfaces were prepared in 120 extracted human molars. Teeth were randomly divided into 5 groups ( n = 24) according to 5 different surface pre-treatments: No pre-treatment (control); 1M carbodiimide (EDC); 0.1% epigallocatechin-3-gallate (EGCG); 2% minocycline (MI); 10% sodium ascorbate (SA). After surface pre-treatment, adhesive (Adper Single Bond 2 [SB], 3M ESPE) was applied. Composite was applied into transparent plastic tubes (2.5 mm in diameter), which was placed over the bonded dentin surface. From each group, 10 samples were subjected to shear bond strength (SBS) evaluation at 24 hours (immediate) and remaining 10 samples were tested after 6 months (delayed). Additionally, 4 samples per group were subjected to scanning electron microscopic analysis for observation of resin-dentin interface. The data were statistically analysed with Shaperio‑Wilk W test, 2-way analysis of variance (ANOVA), and post hoc Tukey's test. At 24 hours, SBS of all surface pre-treatment groups were comparable with the control group, with significant differences found between EDC and SA groups only ( p = 0.009). After 6 months storage, EDC, EGCG, and MI pre-treatments preserved the resin-dentin bond strength with no significant fall. Dentin pre-treatment with all the dentin biomodifiers except SA resulted in significant preservation of resin-dentin bond over 6 months storage period, without negatively affecting the immediate bond strength of the etch and rinse adhesive tested.

  17. Effect of dentin biomodifiers on the immediate and long-term bond strengths of a simplified etch and rinse adhesive to dentin

    Directory of Open Access Journals (Sweden)

    Payal Singh

    2017-07-01

    Full Text Available Objectives This in vitro study evaluated the effect of dentin biomodifiers on the immediate and long-term bond strengths of a simplified etch and rinse adhesive to dentin. Materials and Methods Flat coronal dentin surfaces were prepared in 120 extracted human molars. Teeth were randomly divided into 5 groups (n = 24 according to 5 different surface pre-treatments: No pre-treatment (control; 1M carbodiimide (EDC; 0.1% epigallocatechin-3-gallate (EGCG; 2% minocycline (MI; 10% sodium ascorbate (SA. After surface pre-treatment, adhesive (Adper Single Bond 2 [SB], 3M ESPE was applied. Composite was applied into transparent plastic tubes (2.5 mm in diameter, which was placed over the bonded dentin surface. From each group, 10 samples were subjected to shear bond strength (SBS evaluation at 24 hours (immediate and remaining 10 samples were tested after 6 months (delayed. Additionally, 4 samples per group were subjected to scanning electron microscopic analysis for observation of resin-dentin interface. The data were statistically analysed with Shaperio‑Wilk W test, 2-way analysis of variance (ANOVA, and post hoc Tukey's test. Results At 24 hours, SBS of all surface pre-treatment groups were comparable with the control group, with significant differences found between EDC and SA groups only (p = 0.009. After 6 months storage, EDC, EGCG, and MI pre-treatments preserved the resin-dentin bond strength with no significant fall. Conclusions Dentin pre-treatment with all the dentin biomodifiers except SA resulted in significant preservation of resin-dentin bond over 6 months storage period, without negatively affecting the immediate bond strength of the etch and rinse adhesive tested.

  18. Influence of blood contamination during multimode adhesive application on the microtensile bond strength to dentin.

    Science.gov (United States)

    Kucukyilmaz, E; Celik, E U; Akcay, M; Yasa, B

    2017-12-01

    The present study evaluated the effects of blood contamination performed at different steps of bonding on the microtensile bond strength (μTBS) of multimode adhesives to dentin when using the self-etch approach. Seventy-five molars were randomly assigned to three adhesive groups comprising 25 specimens each: two multimode adhesives [Single Bond Universal (SBU) and All-Bond Universal (ABU)] and a conventional one-step self-etch adhesive [Clearfil S3 Bond Plus (CSBP)]. Each group was subdivided as follows: (1) uncontaminated (control): bonding application/light curing as a positive control; (2) contamination-1 (cont-1): bonding application/light curing/blood contamination/dry as a negative control; (3) contamination-2 (cont-2): bonding application/light curing/blood contamination/rinse/dry; (4) contamination-3 (cont-3): bonding application/blood contamination/dry/bonding re-application/light curing; and (5) contamination-4 (cont-4): bonding application/blood contamination/rinse/dry/bonding re-application/light curing. Dentin specimens were prepared for μTBS testing after the composite resin application. Data were analyzed with two-way ANOVA and post-hoc tests (α = 0.05). μTBS values were similar in cont-3 groups, and ABU/cont-4 and corresponding control groups, but were significantly lower in the other groups than in their control groups (P groups showed the lowest μTBS values (P blood contaminants and reapplying the adhesive may regain the dentin adhesion when contamination occurs before light curing. Alternatively, rinsing and drying contaminants followed by adhesive re-application may be effective depending on adhesive type.

  19. Tensile Bond Strengths of Two Adhesives on Irradiated and Nonirradiated Human Dentin

    Directory of Open Access Journals (Sweden)

    Cécile Bernard

    2015-01-01

    Full Text Available The aim of this study was to assess the effect of radiotherapy on bond efficiency of two different adhesive systems using tensile bond strength test. Twenty extracted teeth after radiotherapy and twenty nonirradiated extracted teeth were used. The irradiation was applied in vivo to a minimal dose of 50 Gy. The specimens of each group were randomly assigned to two subgroups to test two different adhesive systems. A three-step/etch-and-rinse adhesive system (Optibond FL and a two-steps/self-etch adhesive system (Optibond XTR were used. Composite buildups were performed with a nanohybrid composite (Herculite XTR. All specimens were submitted to thermocycling ageing (10000 cycles. The specimens were sectioned in 1 mm2 sticks. Microtensile bond strength tests were measured. Nonparametric statistical analyses were performed due to nonnormality of data. Optibond XTR on irradiated and nonirradiated teeth did not show any significant differences. However, Optibond FL bond strength was more effective on nonirradiated teeth than on irradiated teeth. Within the limitations of an in vitro study, it can be concluded that radiotherapy had a significant detrimental effect on bond strength to human dentin. However, it seems that adhesive choice could be adapted to the substrata. According to the present study, the two-steps/self-etch (Optibond XTR adhesive system tested could be more effective on irradiated dentin compared to three-steps/etch-and-rinse adhesive system (Optibond FL.

  20. Immediate adhesive properties to dentin and enamel of a universal adhesive associated with a hydrophobic resin coat.

    Science.gov (United States)

    Perdigão, J; Muñoz, M A; Sezinando, A; Luque-Martinez, I V; Staichak, R; Reis, A; Loguercio, A D

    2014-01-01

    To evaluate the effect of acid etching and application of a hydrophobic resin coat on the enamel/dentin bond strengths and degree of conversion (DC) within the hybrid layer of a universal adhesive system (G-Bond Plus [GB]). A total of 60 extracted third molars were divided into four groups for bond-strength testing, according to the adhesive strategy: GB applied as a one-step self-etch adhesive (1-stepSE); GB applied as in 1-stepSE followed by one coat of the hydrophobic resin Heliobond (2-stepSE); GB applied as a two-step etch-and-rinse adhesive (2-stepER); GB applied as in 2-stepER followed by one coat of the hydrophobic resin Heliobond (3-stepER). There were 40 teeth used for enamel microshear bond strength (μSBS) and DC; and 20 teeth used for dentin microtensile bond strength (μTBS) and DC. After restorations were constructed, specimens were stored in water (37°C/24 h) and then tested at 0.5 mm/min (μTBS) or 1.0 mm/min (μSBS). Enamel-resin and dentin-resin interfaces from each group were evaluated for DC using micro-Raman spectroscopy. Data were analyzed with two-way analysis of variance for each substrate and the Tukey test (α=0.05). For enamel, the use of a hydrophobic resin coat resulted in statistically significant higher mean enamel μSBS only for the ER strategy (3-stepER vs 2-stepER, penamel etching technique, because it improves bond strengths to enamel when applied with the ER strategy and to dentin when used with the SE adhesion strategy. The application of a hydrophobic resin coat may improve DC in resin-dentin interfaces formed with either the SE or the ER strategy. On enamel, DC may benefit from the application of a hydrophobic resin coat over 1-stepSE adhesives.

  1. Adhesive interfaces of enamel and dentin prepared by air-abrasion at different distances

    International Nuclear Information System (INIS)

    Chinelatti, Michelle Alexandra; Andreolli do Amaral, Thais Helena; Borsatto, Maria Cristina; Palma-Dibb, Regina Guenka; Corona, Silmara Aparecida Milori

    2007-01-01

    The purpose of this study was to analyse, by scanning electron microscopy (SEM), the morphology of enamel and dentin/adhesive interfaces in cavities prepared by air-abrasion at different working distances. Thirty sound third human molars were selected and, on both their buccal and lingual surfaces, class V cavities were prepared by air-abrasion, at 2-, 4-, 6-, 8- and 10-mm working distances, or high-speed bur (control group). After preparation, all cavities were etched with 35% phosphoric acid gel and restored with Single Bond/Filtek Z-250. Buccal and lingual surfaces were separated and restorations sectioned in a buccolingual direction, providing two sections of each cavity, which were analysed by scanning electron microscopy. It was observed that the distances of 6 and 8 mm promoted more homogeneous dentin/adhesive interfaces, with tags formation, and more uniform for enamel, which were similar to the control group. It may be concluded that the air-abrasion working distance can influence the morphology of enamel and dentin/adhesive interfaces, and the intermediate distances provided better adhesive interfaces

  2. [Influence of thermalcycling on bonding durability of self-etch adhesives with dentin].

    Science.gov (United States)

    Tian, Fu-cong; Wang, Xiao-yan; Gao, Xue-jun

    2014-04-18

    To investigate influence of thermalcycling on the bonding durability of two one-step products [Adper Prompt (AP) and G-bond (GB)] and one two-step self-etching adhesive [Clearfil SE bond (SE)] with dentin in vitro. Forty-two extracted human molars were selected. The superficial dentin was exposed by grinding off the enamel. The teeth were randomly distributed into six groups with varied bonding protocols. The adhesives were applied to the dentin surface. Composite crowns were built up, then the samples were cut longitudinally into sticks with 1.0 mm×1.0 mm bonding area [for microtensile bond strength (MTBS) testing] or 1.0 mm thick slabs (for nanoleakage observation). Bonding performance was evaluated with or without thermalcyling. For the MTBS testing, the strength values were statistically analysed using One-Way ANOVA. Four slabs in each group were observed for nanoleakage by SEM with a backscattered electron detector. Thermalcycling procedures affected MTBS. In the two one-step groups, the MTBS decreased significantly (Padhesives showed lower MTBS than two-step bonding system after aging.For AP and GB, continuous nanoleakage appearance was notable and more obvious than for SE. Thermalcycling can affect the bonding performance of self-etch adhesives including decrease of bond strength and nanoleakage pattern. one-step self-etch adhesives showed more obvious change compared with their two-step counterparts.

  3. Investigation of the shear bond strength to dentin of universal adhesives applied with two different techniques

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    Elif Yaşa

    2017-09-01

    Full Text Available Objective: The aim of this study was to evaluate the shear bond strength of universal adhesives applied with self-etch and etch&rinse techniques to dentin. Materials and Method: Fourty-eight sound extracted human third molars were used in this study. Occlusal enamel was removed in order to expose the dentinal surface, and the surface was flattened. Specimens were randomly divided into four groups and were sectioned vestibulo-lingually using a diamond disc. The universal adhesives: All Bond Universal (Group 1a and 1b, Gluma Bond Universal (Group 2a and 2b and Single Bond Universal (Group 3a and 3b were applied onto the tooth specimens either with self-etch technique (a or with etch&rinse technique (b according to the manufacturers’ instructions. Clearfil SE Bond (Group 4a; self-etch and Optibond FL (Group 4b; etch&rinse were used as control groups. Then the specimens were restored with a nanohybrid composite resin (Filtek Z550. After thermocycling, shear bond strength test was performed with a universal test machine at a crosshead speed of 0.5 mm/min. Fracture analysis was done under a stereomicroscope (×40 magnification. Data were analyzed using two-way ANOVA and post-hoc Tukey tests. Results: Statistical analysis showed significant differences in shear bond strength values between the universal adhesives (p<0.05. Significantly higher bond strength values were observed in self-etch groups (a in comparison to etch&rinse groups (b (p<0.05. Among all groups, Single Bond Universal showed the greatest shear bond strength values, whereas All Bond Universal showed the lowest shear bond strength values with both application techniques. Conclusion: Dentin bonding strengths of universal adhesives applied with different techniques may vary depending on the adhesive material. For the universal bonding agents tested in this study, the etch&rinse technique negatively affected the bond strength to dentin.

  4. Effects of chlorhexidine-containing adhesives on the durability of resin-dentine interfaces.

    Science.gov (United States)

    Stanislawczuk, Rodrigo; Pereira, Fabiane; Muñoz, Miguel Angel; Luque, Issis; Farago, Paulo Vitor; Reis, Alessandra; Loguercio, Alessandro D

    2014-01-01

    This study evaluated the effect of addition of diacetate CHX in different concentrations into two simplified etch-and-rinse (ER) adhesive systems (XP Bond [XP] and Ambar {AM}) on the ultimate tensile strength (UTS), degree of conversion (DC), 60-day cumulative water sorption (WS), solubility (SO) and CHX release (CR) as well as the immediate (IM) and 1-year (1Y) resin-dentine bond strength (μTBS) and nanoleakage (NL). Ten experimental adhesive systems were formulated according to the addition of CHX diacetate (0 [control], 0.01, 0.05, 0.1 and 0.2%) in the two ER. For UTS and DC, specimens were constructed and tested after 24h. For WS, SO and CR, after specimens build-up, they were stored in water and the properties measured after 60 days. The occlusal enamel of fifty molars was removed and the adhesives were applied in dentine surface after 37% phosphoric acid etching. After composite resin build-ups, specimens were longitudinally sectioned to obtain resin-dentine bonded sticks (0.8mm(2)). Specimens were tested in tension at 0.5mm/min in the IM or 1Y. For NL, 2 bonded sticks from each tooth were prepared and analyzed under SEM. The data were submitted to appropriate statistical analysis (α=0.05). The addition of CHX did not influence UTS, DC, WS and SO (padhesives with higher concentration of CHX (padhesives or it was less pronounced than the control (XP) regardless of the CHX concentration. The addition of CHX diacetate in concentrations until 0.2% in the simplified ER adhesive systems may be an alternative to increase the long-term stability of resin-dentine interfaces, without jeopardizing the adhesives' mechanical properties evaluated. Copyright © 2013. Published by Elsevier Ltd.

  5. Effect of ethanol-wet bonding with hydrophobic adhesive on caries-affected dentine.

    Science.gov (United States)

    Huang, Xueqing; Li, Li; Huang, Cui; Du, Xijin

    2011-08-01

    Frequently encountered in clinical practice, caries-affected dentine (CAD) is the most challenging bonding substrate. This study evaluated the effect of ethanol-wet bonding with hydrophobic adhesive to sound dentine and to CAD. In the control groups, prepared sound dentine and CAD were bonded with Adper Single Bond 2 using a traditional water-wet bonding technique. In the experimental groups, the specimens were treated as follows: Group 1, rinsed with stepwise ethanol dehydration; Group 2, immersion in 100% ethanol, three times, for 20 s each time; and Group 3, immersion in 100% ethanol for 20 s. Microtensile bond strength (μTBS) testing was used to evaluate the effects of the different protocols on bonding. The microhardness of debonded dentine surfaces was measured to ensure the presence of CAD. Interfacial nanoleakage was evaluated by field-emission scanning electron microscopy. Treatment significantly improved the μTBS in CAD in Groups 1 and 2, but had no effect on Group 3. Conversely, treatment significantly reduced the μTBS in sound dentine in Groups 2 and 3, but had no effect in Group 1. The presence of nanoleakage varied with the ethanol-wet protocol used. In conclusion, ethanol-wet bonding can potentially improve bond efficacy to CAD when an appropriate protocol is used. © 2011 Eur J Oral Sci.

  6. Effect of Enamel and Dentin Surface Treatment on the Self-Adhesive Resin Cement Bond Strength.

    Science.gov (United States)

    Mushashe, Amanda Mahmmad; Gonzaga, Carla Castiglia; Cunha, Leonardo Fernandes da; Furuse, Adilson Yoshio; Moro, Alexandre; Correr, Gisele Maria

    2016-01-01

    The aim of this study was to evaluate the effect of enamel and dentin surface treatment on the micro-shear bond strength of self-adhesive cement. Seventy-two extracted third molars had their crowns embedded in acrylic resin and worn to obtain a flat enamel or dentin surface. The enamel and dentin specimens were randomly assigned to 8 groups (n=12) that were based on surface treatment (11.5% polyacrylic acid solution or no treatment), substrate condition (wet or dry) and storage period (1 day or 90 days), and treated accordingly. Cylinders (1 × 1 mm) were fabricated using self-adhesive resin cement (RelyX U200) following the manufacturer's instructions. The specimens were stored in distilled water at 37 °C for either 1 day or 90 days and subjected to micro-shear bond strength test (EMIC DL 2000 at 0.5 mm/min). After this, the failure type of the specimens was determined. Data were subjected to statistical analysis (a=0.05). According to the results, the 11.5% polyacrylic acid application decreased the bond strength in both enamel and dentin samples. The moist groups showed higher bond strength than the dry ones, regardless of the substrate and surface treatment. Storage period did not influence bond strength. In conclusion, surface treatment with 11.5% polyacrylic acid and absence of moisture decreased the bond strength of the resin-cement (RelyU200), regardless of the storage period.

  7. Resin-dentin Bond Stability of Experimental 4-META-based Etch-and-rinse Adhesives Solvated by Ethanol or Acetone.

    Science.gov (United States)

    Amaral, Cristiane Mariote; Diniz, Alice Marques; Arantes, Eugênio Braz Rodrigues; Dos Santos, Glauco Botelho; Noronha-Filho, Jaime Dutra; da Silva, Eduardo Moreira

    To investigate the influence of 4-META concentration and type of solvent on the degree of conversion (DC%) and resin-dentin bond stability of experimental etch-and-rinse adhesives. Four different concentrations of 4-META (12 wt%, 20 wt%, 30 wt%, 40 wt%) were added to a model adhesive system consisting of TEG-DMA (25 wt%), UDMA (20 wt%), HEMA (30 wt%), water (4 wt%), camphorquinone (0.5 wt%), and tertiary amine (0.5 wt%) dissolved in 20% acetone (A12, A20, A30 and A40) or 20% ethanol (E12, E20, E30 and E40). DC% was evaluated by FT-IR spectroscopy. Human molars were wet ground until the occlusal dentin was exposed, the adhesive systems were applied after 37% phosphoric acid etching, and resin composite buildups were incrementally constructed. After storage in distilled water at 37°C for 24 h, the teeth were cut into resin-dentin beams (cross-sectional area 1 mm2). Microtensile bond strength (μTBS) was evaluated after 24 h, 6 months, and 1 year of water storage at 37°C. The failure mode was categorized as adhesive, mixed, or cohesive. Data were analyzed using ANOVA and Tukey's HSD test (α = 0.05). A12 presented the lowest DC% (p 0.05). All adhesive systems maintained resin-dentin bond stability after 6 months of water storage, while only A40 and E40 maintained it after 1 year. Irrespective of the type of organic solvent, the incorporation of high concentrations of 4-META (40 wt%) improved the resin-dentin bond stability of the experimental etch-and-rinse adhesive systems over a period of 1 year.

  8. Hybridization quality and bond strength of adhesive systems according to interaction with dentin.

    Science.gov (United States)

    Salvio, Luciana Andrea; Hipólito, Vinicius Di; Martins, Adriano Luis; de Goes, Mario Fernando

    2013-07-01

    To evaluate the hybridization quality and bond strength of adhesives to dentin. Ten human molars were ground to expose the dentin and then sectioned in four tooth-quarters. They were randomly divided into 5 groups according to the adhesive used: Two single-step self-etch adhesives - Adper Prompt (ADP) and Xeno III (XE), two two-step self-etching primer systems - Clearfil SE Bond (SE) and Adhe SE (ADSE), and one one-step etch-and-rinse system - Adper Single Bond (SB). Resin composite (Filtek Z250) crown buildups were made on the bonded surfaces and incrementally light-cured for 20 s. The restored tooth-quarters were stored in water at 37°C for 24 h and then sectioned into beams (0.8 mm(2) in cross-section). Maximal microtensile bond strength (μ-TBS) was recorded (0.5 mm/min in crosshead speed). The results were submitted to one-way ANOVA and Tukey's test (α = 0.05). Thirty additional teeth were used to investigate the hybridization quality by SEM using silver methenamine or ammoniacal silver nitrate dyes. SE reached significantly higher μ-TBS (P 0.05), and between SB and ADP (P > 0.05); ADSE and XE were significantly higher than SB and ADP (P quality than that observed for ADP and XE. The bond strength and hybridization quality were affected by the interaction form of the adhesives with dentin. The hybridization quality was essential to improve the immediate μ-TBS to dentin.

  9. Durable bonds at the adhesive/dentin interface: an impossible mission or simply a moving target?

    Science.gov (United States)

    SPENCER, Paulette; Jonggu PARK, Qiang YE; MISRA, Anil; BOHATY, Brenda S.; SINGH, Viraj; PARTHASARATHY, Ranga; SENE, Fábio; de Paiva GONÇALVES, Sérgio Eduardo; LAURENCE, Jennifer

    2013-01-01

    Composite restorations have higher failure rates, more recurrent caries and increased frequency of replacement as compared to dental amalgam. Penetration of bacterial enzymes, oral fluids, and bacteria into the crevices between the tooth and composite undermines the restoration and leads to recurrent decay and failure. The gingival margin of composite restora tions is particularly vulnerable to decay and at this margin, the adhesive and its seal to dentin provides the primary barrier between the prepared tooth and the environment. The intent of this article is to examine physico-chemical factors that affect the integrity and durability of the adhesive/dentin interfacial bond; and to explore how these factors act synergistically with mechanical forces to undermine the composite restoration. The article will examine the various avenues that have been pursued to address these problems and it will explore how alterations in material chemistry could address the detrimental impact of physico-chemical stresses on the bond formed at the adhesive/dentin interface. PMID:24855586

  10. Effect of tetracycline on the bond performance of etch-and-rinse adhesives to dentin

    Directory of Open Access Journals (Sweden)

    Rodrigo Stanislawczuk

    2011-10-01

    Full Text Available This study evaluated the effect of modified tetracycline on the resin-dentin bond strength (µTBS, silver nitrate uptake (SNU and solution homogeneity (SH of two adhesives. Dentin surfaces were treated with phosphoric acid, rinsed off and either rewetted with water (control group - CO, 2% minocycline (MI, 2% doxycyline (DO or 2% chlorhexidine (CH. Adhesive systems (Adper Single Bond 2 and Prime Bond NT and composite were applied and light-polymerized. Specimens were sectioned to obtain bonded sticks (0.8 mm² to test under tension at 0.5 mm/min. For SNU, specimens were immersed in silver nitrate and analyzed by EDX-SEM. SH was qualitatively analyzed after mixing the adhesives with different solvent-based solutions containing MI, DO and CH. Lower µTBS values were observed in the DO group compared with MI and CH (p = 0.01. Lower SNU was observed for MI and CH. The lowest µTBS for both adhesives was observed for the DO group (p = 0.01. Signs of phase separation were observed for DO with both adhesives. MI or CH used as rewetting solutions after acid etching did not affect the µTBS and hybrid layer quality.

  11. Influence of temporary cement contamination on the surface free energy and dentine bond strength of self-adhesive cements.

    Science.gov (United States)

    Takimoto, Masayuki; Ishii, Ryo; Iino, Masayoshi; Shimizu, Yusuke; Tsujimoto, Akimasa; Takamizawa, Toshiki; Ando, Susumu; Miyazaki, Masashi

    2012-02-01

    The surface free energy and dentine bond strength of self-adhesive cements were examined after the removal of temporary cements. The labial dentine surfaces of bovine mandibular incisors were wet ground with #600-grit SiC paper. Acrylic resin blocks were luted to the prepared dentine surfaces using HY Bond Temporary Cement Hard (HY), IP Temp Cement (IP), Fuji TEMP (FT) or Freegenol Temporary Cement (TC), and stored for 1 week. After removal of the temporary cements with an ultrasonic tip, the contact angle values of five specimens per test group were determined for the three test liquids, and the surface-energy parameters of the dentine surfaces were calculated. The dentine bond strengths of the self-adhesive cements were measured after removal of the temporary cements in a shear mode at a crosshead speed of 1.0mm/min. The data were subjected to one-way analysis of variance (ANOVA) followed by Tukey's HSD test. For all surfaces, the value of the estimated surface tension component γ(S)(d) (dispersion) was relatively constant at 41.7-43.3 mJm(-2). After removal of the temporary cements, the value of the γ(S)(h) (hydrogen-bonding) component decreased, particularly with FT and TC. The dentine bond strength of the self-adhesive cements was significantly higher for those without temporary cement contamination (8.2-10.6 MPa) than for those with temporary cement contamination (4.3-7.1 MPa). The γ(S) values decreased due to the decrease of γ(S)(h) values for the temporary cement-contaminated dentine. Contamination with temporary cements led to lower dentine bond strength. The presence of temporary cement interferes with the bonding performance of self-adhesive cements to dentine. Care should be taken in the methods of removal of temporary cement when using self-adhesive cements. Copyright © 2011 Elsevier Ltd. All rights reserved.

  12. Bond Stability of a Universal Adhesive System to Eroded/Abraded Dentin After Deproteinization.

    Science.gov (United States)

    Augusto, M G; Torres, Crg; Pucci, C R; Schlueter, N; Borges, A B

    Erosive/abrasive challenges can potentially compromise bonding to dentin. Aiming to improve the quality and stability of bonding to this substrate, this study investigated the combined effect of erosion and toothbrush abrasion on the microtensile bond strength (μTBS) stability to dentin using a universal adhesive system in total and self-etching modes, associated or not associated with deproteinization. Bovine dentin specimens were divided into five groups according to the organic matrix condition (n=20): control (C); erosion (E); erosion + abrasion (EA); erosion + sodium hypochlorite (EH); erosion + abrasion + sodium hypochlorite (EAH). The groups were further divided (n=10) according to the mode of application (total or self-etching) of a universal adhesive. After the bonding procedure, composite blocks were built up, and the samples were cut to obtain sticks for μTBS testing. For each specimen, one-half of the sticks was immediately tested, and the other one-half was tested after artificial aging (5000 thermocycles, 5°C and 55°C). Three-way analysis of variance (α=5%) showed a significant difference for the triple interaction ( p=0.0007). Higher μTBS means were obtained for the EH and EAH groups compared with the E and EA groups. The control group showed immediate μTBS values similar to that of the E and EA groups for both bond strategies. Erosion and erosion/abrasion did not significantly influence the immediate μTBS to dentin. Artificial aging reduced μTBS values for the groups C, E, and EA using the total-etching mode. Deproteinization maintained the bond stability to artificially aged eroded and eroded/abraded dentin.

  13. Degradation of Multimode Adhesive System Bond Strength to Artificial Caries-Affected Dentin Due to Water Storage.

    Science.gov (United States)

    Follak, A C; Miotti, L L; Lenzi, T L; Rocha, R O; Soares, F Z

    The purpose of this study was to evaluate the influence of water storage on bond strength of multimode adhesive systems to artificially induced caries-affected dentin. One hundred twelve sound bovine incisors were randomly assigned to 16 groups (n=7) according to the dentin condition (sound; SND, artificially induced caries-affected dentin; CAD, cariogenic challenge by pH cycling for 14 days); the adhesive system (SU, Scotchbond Universal Adhesive; AB, All-Bond Universal; PB, Prime & Bond Elect; SB, Adper Single Bond 2; and CS, Clearfil SE Bond), and the etching strategy (etch-and-rinse and self-etch). All adhesive systems were applied under manufacturer's instructions to flat dentin surfaces, and a composite block was built up on each dentin surface. After 24 hours of water storage, the specimens were sectioned into stick-shaped specimens (0.8 mm 2 ) and submitted to a microtensile test immediately (24 hours) or after six months of water storage. Bond strength data (MPa) were analyzed using three-way repeated-measures analysis of variance and post hoc Tukey test (α=5%), considering each substrate separately (SND and CAD). The etching strategy did not influence the bond strength of multimode adhesives, irrespective of the dentin condition. Water storage only reduced significantly the bond strength to CAD. The degradation of bond strength due to water storage was more pronounced in CAD, regardless of the etching strategy.

  14. Bonding performance of self-adhesive flowable composites to enamel, dentin and a nano-hybrid composite.

    Science.gov (United States)

    Peterson, Jana; Rizk, Marta; Hoch, Monika; Wiegand, Annette

    2018-04-01

    This study aimed to analyze bond strengths of self-adhesive flowable composites on enamel, dentin and nano-hybrid composite. Enamel, dentin and nano-hybrid composite (Venus Diamond, Heraeus Kulzer, Germany) specimens were prepared. Three self-adhesive composites (Constic, DMG, Germany; Fusio Liquid Dentin, Pentron Clinical, USA; Vertise Flow, Kerr Dental, Italy) or a conventional flowable composite (Venus Diamond Flow, Heraeus Kulzer, Germany, etch&rinse technique) were applied to enamel and dentin. Nano-hybrid composite specimens were initially aged by thermal cycling (5000 cycles, 5-55 °C). Surfaces were left untreated or pretreated by mechanical roughening, Al 2 O 3 air abrasion or silica coating/silanization. In half of the composite specimens, an adhesive (Optibond FL, Kerr Dental, Italy) was used prior to the application of the flowable composites. Following thermal cycling (5000 cycles, 5-55 °C) of all specimens, shear bond strengths (SBS) and failure modes were analyzed (each subgroup n = 16). Statistical analysis was performed by ANOVAs/Bonferroni post hoc tests, Weibull statistics and χ 2 -tests (p composites on enamel and dentin were significantly lower (enamel: composite (enamel: 13.0 ± 5.1, dentin: 11.2 ± 6.3), and merely adhesive failures could be observed. On the nano-hybrid composite, SBS were significantly related to the pretreatment. Adhesive application improved SBS of the conventional, but not of the self-adhesive composites. The self-adhesive composite groups showed less cohesive failures than the reference group; the occurence of cohesive failures increased after surface pretreatment. Bonding of self-adhesive flowable composites to enamel and dentin is lower than bonding to a nano-hybrid composite.

  15. Influence of dentinal regions on bond strengths of different adhesive systems.

    Science.gov (United States)

    Ozer, F; Unlü, N; Sengun, A

    2003-06-01

    This in vitro study assessed comparatively the shear bond strengths of three composite resins, 3M Valux Plus (3MVP), Herculite (H), Clearfil AP-X (CAP-X), a polyacid modified composite resin Dyract (D), and a resin modified glass-ionomer materials Vitremer (V), to cervical and buccal dentine regions of extracted human molar teeth. Four different bonding systems, 3M ScotchBond Multipurpose (SB), Clearfil Liner Bond 2 (LB2), Opti Bond (OB), and Prime & Bond 2.1 (PB 2.1) were used with the manufacturer's respective composite and compomer materials. One hundred freshly extracted mandibular molar teeth were selected for this study. Flat buccal dentine surfaces were created on 50 teeth and cylindrical rods of the five materials were bonded to the dentine surfaces. For assessment of cervical bond strengths, the materials were bonded to mesial and distal enamel bordered occlusal dentinal surfaces of the remaining 50 teeth. The five groups of restorative procedures were applied as follows; Group 1: SB + 3MVP, Group 2: LB2 + CAP-X, Group 3: OB + H, Group 4: PB2.1 + D, Group 5: Vitremer primer (VP) VP + V. Each restorative procedure thus had 20 specimens (10 buccal + 10 cervical). After 24 h of water storage (37 degrees C), the specimens were tested on a Universal Testing machine in shear with a cross head speed of 0.5 mm min-1. The bond strength values were calculated in MPa and the results were evaluated statistically using Kruskal-Wallis one-way/anova and Mann-Whitney U-tests. It was found that the bond strengths of SB + 3MVP, LB2 + CAP-X and VP + V to buccal dentine surfaces were significantly stronger (P 0.05). Vitremer was found the least successful adhesive material in terms of shear bond strength on both buccal and occluso-cervical dentine surfaces.

  16. Shear bond strength of one-step self-etch adhesives to dentin: Evaluation of NaOCl pretreatment.

    Science.gov (United States)

    Colombo, Marco; Beltrami, Riccardo; Chiesa, Marco; Poggio, Claudio; Scribante, Andrea

    2018-02-01

    The aim of this study was to evaluate the influence of dentin pretreatment with NaOCl on shear bond strength of four one-step self-etch adhesives with different pH values. Bovine permanent incisors were used. Four one-step self-etch adhesives were tested: Adper™ Easy Bond, Futurabond NR, G-aenial Bond, Clearfil S3 Bond. One two-step self-etch adhesive (Clearfil SE Bond) was used as control. Group 1- no pretreatment; group 2- pretratment with 5,25 % NaOCl; group 3- pretreatment with 37 % H3PO4 etching and 5,25 % NaOCl. A hybrid composite resin was inserted into the dentin surface. The specimens were tested in a universal testing machine. The examiners evaluated the fractured surfaces in optical microscope to determine failure modes, quantified with adhesive remnant index (ARI). Dentin pretreatment variably influenced bond strength values of the different adhesive systems. When no dentin pretreatment was applied, no significant differences were found ( P >.05) among four adhesives tested. No significant differences were recorded when comparing NaOCl pretreatment with H3PO4 + NaOCl pretreatment for all adhesive tested ( P >.05) except Clearfil S3 Bond that showed higher shear bond strength values when H3PO4 was applied. Frequencies of ARI scores were calculated. The influence of dentin pretreatment with NaOCl depends on the composition of each adhesive system used. There was no difference in bond strength values among self-etch adhesives with different pH values. Key words: Dentin, pretreatment, self-etch adhesives.

  17. BOND STRENGTH OF SELF-ETCH ADHESIVES WITH PRIMARY AND PERMANENT TEETH DENTIN – IN VITRO STUDY.

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    Natalia Gateva

    2012-03-01

    Full Text Available Objective: The aim of this study was to compare dentin bond strength of primary and permanent teeth with self-etching adhesive systems. Methods: On 40 intact specimens of primary and permanent teeth was created flat dentin surfaces. The patterns were divided in 4 groups. Two different self-etching adhesive systems were used – one two steps (AdheSE, VivaDent and one one step (AdheSE One, VivaDent. Resin composite build-ups were constructed by means of convetional copper ring after applying the adhesive. The specimens were stored in water for 72 h at room temperature. After that specimens were tested for macrotensile bond strength. Debonded surfaces were analyzed by SEM.Conclusions: The measured values of dentin bond strength after applying self-etching adhesives are statistically significant in group of permanent teeth in comparison with group of primary teeth, and for both adhesive generations. Two steps self-etching adhesive provide significant stronger dentin bond strength with both dentitions in comparison with one step self-etching adhesive.

  18. Effect of etching on bonding of a self-etch adhesive to dentine affected by amelogenesis imperfecta.

    Science.gov (United States)

    Epasinghe, Don Jeevanie; Yiu, Cynthia Kar Yung

    2018-02-01

    Dentine affected by amelogenesis imperfecta (AI) is histologically altered due to loss of hypoplastic enamel and becomes hypermineralized. In the present study, we examined the effect of additional acid etching on microtensile bond strength of a self-etch adhesive to AI-affected dentine. Flat coronal dentine obtained from extracted AI-affected and non-carious permanent molars were allocated to two groups: (a) Clearfil SE Bond (control); and (b) Clearfil SE Bond and additional etching with 34% phosphoric acid for 15 seconds. The bonded teeth were sectioned into .8-mm 2 beams for microtensile bond strength testing, and stressed to failure under tension. The bond strength data were analyzed using two-way analysis of variance (dentine type and etching step) and Student-Newman-Keuls multiple comparison test (P<.05). Representative fractured beams from each group were examined under scanning electron microscopy. Both factors, dentine substrate (P<.001) and etching step (P<.05), and their interactions (P<.001), were statistically significant. Additional etching had an adverse effect on the bond strength of Clearfil SE Bond to normal dentine (P<.005), and no significant improvement was found for AI-affected dentine (P=.479). Additional acid etching does not improve the bond strength of a self-etch adhesive to AI-affected dentine. © 2017 John Wiley & Sons Australia, Ltd.

  19. Pre-treating dentin with chlorhexadine and CPP-ACP: self-etching and universal adhesive systems.

    Science.gov (United States)

    Dos Santos, Ricardo Alves; de Lima, Eliane Alves; Montes, Marcos Antônio Japiassu Resende; Braz, Rodivan

    2016-12-01

    Objective: The aim of the present study was to compare the effect of pre-treating dentin with chlorhexidine, at concentrations of 0.2% and 2%, and remineralizing paste containing CPP-ACP (MI Paste - GC) on the bond strength of adhesive systems. Material and methods: In total, 80 slides of dentin were used. These slides were 2 mm thick and were obtained from bovine incisors. Standard cavities were created using diamond bur number 3131. In the control groups, a Scotchbond Universal Adhesive (SUA) self-etching adhesive system of 3M ESPE and a Clearfil SE Bond (CSE) adhesive system of Kuraray were applied, following the manufacturer's instructions. In the other groups, dentin was pretreated with chlorhexidine (0.2% and 2%) for 1 min and with MI Paste for 3 min. The cavities were restored with Z350 XT resin (3M ESPE). After 24 h of storage, the push-out test was applied at a speed of 0.5 mm/min. Results: The different dentin pretreatment techniques did not affect the intra-adhesive bond strength. There was a difference between treatment with MI Paste and chlorhexidine 0.2% in favor of the SUA, with values of 15.22 and 20.25 Mpa, respectively. Conclusions: The different pretreatment methods did not alter the immediate bond strength to dentin. Differences were only recorded when comparing the adhesives.

  20. Influence of intrapulpal pressure simulation on the bond strength of adhesive systems to dentin

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    Marcio Vivan Cardoso

    2008-06-01

    Full Text Available The purpose of this study was to evaluate the influence of intrapulpal pressure simulation on the bonding effectiveness of etch & rinse and self-etch adhesives to dentin. Eighty sound human molars were distributed into eight groups, according to the permeability level of each sample, measured by an apparatus to assess hydraulic conductance (Lp. Thus, a similar mean permeability was achieved in each group. Three etch & rinse adhesives (Prime & Bond NT - PB, Single Bond -SB, and Excite - EX and one self-etch system (Clearfil SE Bond - SE were employed, varying the presence or absence of an intrapulpal pressure (IPP simulation of 15 cmH2O. After adhesive and restorative procedures were carried out, the samples were stored in distilled water for 24 hours at 37°C, and taken for tensile bond strength (TBS testing. Fracture analysis was performed using a light microscope at 40 X magnification. The data, obtained in MPa, were then submitted to the Kruskal-Wallis test ( a = 0.05. The results revealed that the TBS of SB and EX was significantly reduced under IPP simulation, differing from the TBS of PB and SE. Moreover, SE obtained the highest bond strength values in the presence of IPP. It could be concluded that IPP simulation can influence the bond strength of certain adhesive systems to dentin and should be considered when in vitro studies are conducted.

  1. Six-month storage-time evaluation of one-bottle adhesive systems to dentin.

    Science.gov (United States)

    Giannini, Marcelo; Seixas, Carla Alessandra Marcelino; Reis, Andre Figueiredo; Pimenta, Luiz André Freire

    2003-01-01

    The goal of this study was to evaluate the 1-week, 3-month, and 6-month performance of eight commercially available one-bottle adhesive systems to dentin. Lingual and buccal surfaces from human third molars were ground wet on 600-grit SiC paper to obtain a flat dentinal surface. The specimens were randomly divided into 24 groups (n = 10), which were established to measure the shear bond strengths of Bond-1 (B1), ONE-STEP (OS), OptiBond SOLO (OP), Prime & Bond 2.1 (PB), Single Bond (SB), STAE (ST), Syntac Sprint (SS), and Tenure Quick (TQ) after 1-week, 3-month, and 6-month water storage at 37 degrees C. One-bottle adhesives were applied according to manufacturers' instructions and Z100 composite cylinders were applied on the bonded dentinal surfaces. The 3-month water-storage groups were thermocycled for 1500 cycles at 5 degrees C and 55 degrees C and 6-month groups for 3000 cycles. After storage periods, specimens were tested in shear in a universal testing machine (0.5 mm/min). were statistically analyzed by analysis of variance and Tukey test. Results: The changes in shear bond strengths were not uniform over time. Over the test period, OS, PB, SB, and SS exhibited bond strength stability, however, SS presented low bond strengths on all tested periods. A significant decrease in bond strength was observed for B1, OP, ST, and TQ after the 6-month storage period.

  2. Shear bond strength of different adhesive systems to normal and caries-affected dentin

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    Niloofar Shadman

    2015-10-01

    Full Text Available BACKGROUND AND AIM: According to the effect of the adhesive and substrate type on the bond strength, examination of the adhesive is required in all aspects. The aim of this study was to evaluate the shear bond strength of different adhesive systems to normal dentin (ND and caries affected dentin (CAD in permanent teeth. METHODS: Thirty extracted molars with small occlusal caries were selected. After preparation and determination of ND and CAD by caries detector, teeth were divided into three groups and treated with one of the two tested adhesives: Single Bond 2 (SB2, Scotchbond Universal with etch (SBU-ER, and Scotchbond Universal without etch (SBU-SE. Then composite (Filtek Z-250 XT were attached to the surfaces and cured. After water storage (24 hours and thermocycling (500 cycles 5-55 °C, bond strength was calculated and failure modes were determined by stereomicroscope. The data were analyzed by one-way ANOVA and post-hoc test [Tukey HSD (honest significant difference] and with P ˂ 0.050 as the level of significance. RESULTS: Only SBU-ER had significantly higher shear bond strength than SBU-SE in ND (P = 0.027 and CAD (P = 0.046. Bond strength in SBU-ER the highest and in SBU-SE had the lowest amounts in CAD and ND. There was no significant difference in each group between ND and CAD. CONCLUSION: The 2-step etch-and-rinse adhesive (SBU-ER had higher bond strength to ND and CAD than the selfetch adhesive (SBU-SE.

  3. Influence of different etching modes on bond strength and fatigue strength to dentin using universal adhesive systems.

    Science.gov (United States)

    Takamizawa, Toshiki; Barkmeier, Wayne W; Tsujimoto, Akimasa; Berry, Thomas P; Watanabe, Hedehiko; Erickson, Robert L; Latta, Mark A; Miyazaki, Masashi

    2016-02-01

    The purpose of this study was to determine the dentin bonding ability of three new universal adhesive systems under different etching modes using fatigue testing. Prime & Bond elect [PE] (DENTSPLY Caulk), Scotchbond Universal [SU] (3M ESPE), and All Bond Universal [AU] (Bisco) were used in this study. A conventional single-step self-etch adhesive, Clearfil Bond SE ONE [CS] (Kuraray Noritake Dental) was also included as a control. Shear bond strengths (SBS) and shear fatigue strength (SFS) to human dentin were obtained in the total-etch mode and self-etch modes. For each test condition, 15 specimens were prepared for the SBS and 30 specimens for SFS. SEM was used to examine representative de-bonded specimens, treated dentin surfaces and the resin/dentin interface for each test condition. Among the universal adhesives, PE in total-etch mode showed significantly higher SBS and SFS values than in self-etch mode. SU and AU did not show any significant difference in SBS and SFS between the total-etch mode and self-etch mode. However, the single-step self-etch adhesive CS showed significantly lower SBS and SFS values in the etch-and-rinse mode when compared to the self-etch mode. Examining the ratio of SFS/SBS, for PE and AU, the etch-and-rinse mode groups showed higher ratios than the self-etch mode groups. The influence of different etching modes on dentin bond quality of universal adhesives was dependent on the adhesive material. However, for the universal adhesives, using the total-etch mode did not have a negative impact on dentin bond quality. Copyright © 2015 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

  4. Effect of Storage Time on Bond Strength and Nanoleakage Expression of Universal Adhesives Bonded to Dentin and Etched Enamel.

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    Makishi, P; André, C B; Ayres, Apa; Martins, A L; Giannini, M

    2016-01-01

    To investigate bond strength and nanoleakage expression of universal adhesives (UA) bonded to dentin and etched enamel. Extracted human third molars were sectioned and ground to obtain flat surfaces of dentin (n = 36) and enamel (n = 48). Dentin and etched enamel surfaces were bonded with one of two UAs, All-Bond Universal (ABU) or Scotchbond Universal (SBU); or a two-step self-etching adhesive, Clearfil SE Bond (CSEB). A hydrophobic bonding resin, Adper Scotchbond Multi-Purpose Bond (ASMP Bond) was applied only on etched enamel. Following each bonding procedure, resin composite blocks were built up incrementally. The specimens were sectioned and subjected to microtensile bond strength (MTBS) testing after 24 hours or one year water storage, or immersed into ammoniacal silver nitrate solution after aging with 10,000 thermocycles and observed using scanning electron microscopy. The percentage distribution of silver particles at the adhesive/tooth interface was calculated using digital image-analysis software. The MTBS (CSEB = SBU > ABU, for dentin; and CSEB > ABU = SBU = ASMP Bond, for etched enamel) differed significantly between the adhesives after 24 hours. After one year, MTBS values were reduced significantly within the same adhesive for both substrates (analysis of variance, Bonferroni post hoc, padhesives for etched enamel. Silver particles could be detected within the adhesive/dentin interface of all specimens tested. Kruskal-Wallis mean ranks for nanoleakage in ABU, SBU, and CSEB were 16.9, 18.5 and 11, respectively (p>0.05). In the short term, MTBS values were material and dental-substrate dependent. After aging, a decrease in bonding effectiveness was observed in all materials, with nanoleakage at the adhesive/dentin interface. The bonding of the UAs was equal or inferior to that of the conventional restorative systems when applied to either substrate and after either storage period.

  5. Influence of dentin contamination by temporary cements on the bond strength of adhesive systems

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    Josimeri Hebling

    2009-01-01

    Full Text Available Objective: The aim of this study was to assess the bond strength of adhesive systems to dentin contaminated by temporary cements with or without eugenol. Method: Flat dentin surfaces were obtained from twenty-four human third molars. With exception of the control group (n=8, the surfaces were covered with Interim Restorative Material (Caulk Dentsplay, Milford, DE, USA or Cavit (3M ESPE, St. Paul, MN, USA and kept in an oven at 37oC for seven days. After removing the cements, the adhesive systems Adper Single Bond (3M ESPE, St. Paul, MN, USA or Clearfil SE Bond (Kuraray Co. Ltd., Osaka, Japan were applied in accordance with the manufacturers’ recommendations, and then the crowns were constructed in of resin composite. The teeth were sectioned into specimens with a cross-sectional bond area of 0.81mm2, which were sub mitted to microtensile testing in a mechanical test machine at an actuator speed of 0.5mm/min. The data were analyzed by t- and ANOVA tests, complemented by Tukey tests (α=0.05. Results: For Adper Single Bond (3M ESPE, St. Paul, MN, USA, bond strength did not differ statistically (p>0.05 for all the experimental conditions. For Clearfil SE Bond (Kuraray Co. Ltd., Osaka, Japan, only the Interim Restorative Material (Caulk Dentsplay, Milford, DE, USA Group showed significantly lower bond strength (30.1±13.8 MPa in comparison with the other groups; control (38.9±13.5 MPa and Cavit (3M ESPE, St. Paul, MN, USA (42.1±11.0 MPa, which showed no significant difference between them.Conclusion: It was concluded that the previous covering of dentin with temporary cement containing eugenol had a deleterious effect on the adhesive performance of the self-etching system only.

  6. Bond strength of an adhesive system irradiated with Nd:YAG laser in dentin treated with Er:YAG laser

    International Nuclear Information System (INIS)

    Malta, D A M P; De Andrade, M F; Costa, M M; Lizarelli, R F Z; Pelino, J E P

    2008-01-01

    The purpose of this in vitro study was to verify through micro tensile bond test the bond strength of an adhesive system irradiated with Nd:YAG laser in dentine previously treated with Er:YAG laser. Twenty caries free extracted human third molars were used. The teeth were divided in four experimental groups (n = 5): (G1) control group; (G2) irradiation of the adhesive system with the Nd:YAG laser; (G3) dentin treatment with Er:YAG laser; (G4) dentin treatment with Er:YAG laser followed by the irradiation of the adhesive system with Nd:YAG laser. The Er:YAG laser fluency parameter for the dentin treatment was of 60 J/cm 2 . The adhesive system was irradiated with the Nd:YAG laser with fluency of 100 J/cm 2 . Dental restorations were performed with Adper Single Bond 2/Z250. One tooth from each group was prepared for the evaluation of the adhesive interface under SEM and bond failure tests were also performed and evaluated. The statistical analysis showed statistical significant difference between the groups G1 and G3, G1 and G4, G2 and G3, and G2 and G4; and similarity between the groups G1 and G2, and G3 and G4. The adhesive failures were predominant in all the experimental groups. The SEM analysis showed an adhesive interface with features confirming the results of the mechanical tests. The Nd:YAG laser on the adhesive system did not influence the bond strength in dentin treated or not with the Er:YAG laser

  7. A study on the compatibility between one-bottle dentin adhesives and composite resins using micro-shear bond strength.

    Science.gov (United States)

    Song, Minju; Shin, Yooseok; Park, Jeong-Won; Roh, Byoung-Duck

    2015-02-01

    This study was performed to determine whether the combined use of one-bottle self-etch adhesives and composite resins from same manufacturers have better bond strengths than combinations of adhesive and resins from different manufacturers. 25 experimental micro-shear bond test groups were made from combinations of five dentin adhesives and five composite resins with extracted human molars stored in saline for 24 hr. Testing was performed using the wire-loop method and a universal testing machine. Bond strength data was statistically analyzed using two way analysis of variance (ANOVA) and Tukey's post hoc test. Two way ANOVA revealed significant differences for the factors of dentin adhesives and composite resins, and significant interaction effect (p composite resin (p composite resin than other manufacturer's composite resin. Not all combinations of adhesive and composite resin by same manufacturers failed to show significantly higher bond strengths than mixed manufacturer combinations.

  8. Effect of antioxidants on the dentin interface bond stability of adhesives exposed to hydrolytic degradation.

    Science.gov (United States)

    Gotti, Valéria B; Feitosa, Victor P; Sauro, Salvatore; Correr-Sobrinho, Lourenço; Leal, Fernanda B; Stansbury, Jeffrey W; Correr, Américo B

    2015-02-01

    This study assessed the effect of antioxidants vitamin C (Vit. C), vitamin E (Vit. E) and quercetin (Querc) on the dentin bonding performance, degree of conversion, and rate of polymerization of three commercial adhesive systems (Adper Single Bond 2 [SB], Clearfil SE Bond [CSE], Adper Easy Bond [EB]). Human premolars were restored using antioxidant-doped adhesives. The samples were stored for 24 h in distilled water or 6 months under simulated pulpal pressure. Teeth were cut into sticks and the microtensile bond strength (μTBS) to dentin was tested in a universal testing machine. Qualitative nanoleakage analysis was performed from a central stick of each restored tooth. Degree of conversion and rate of polymerization of adhesive systems were evaluated in triplicate using real-time FT-IR. Although the inclusion of the antioxidants negatively affected the μTBS over 24 h, the antioxidant-doped adhesives maintained (SB-Vit. C, SB-Vit. E, CSE-Vit. C, EB-Querc) or increased (SB-Querc, CSE-Vit. E, CSE-Querc, EB-Vit. E, and EB-Vit. C) their μTBS during 6 months of storage. Only the μTBS of Adper Single Bond 2 dropped significantly after 6 months among the control groups. Slight changes in the nanoleakage pattern after aging were observed in all groups, except for the EB-control group, which showed a noteworthy increase in nanoleakage after 6 months, and for EB-Vit. C, which presented a remarkable decrease. A lower degree of conversion was obtained with all antioxidants in SB and EB, except for the EB-Vit. E group. Similar degrees of conversion were attained in control and experimental groups for CSE. The rate of polymerization was reduced in antioxidant-doped adhesives. The performance of antioxidants changed according to the adhesive system to which they were added, and antioxidant-doped adhesives appear to have a positive effect on the adhesive interface durability, since their bond strength obtained after 24 h was maintained or increased over time.

  9. Tensile bond strength of indirect composites luted with three new self-adhesive resin cements to dentin

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    Cafer Türkmen

    2011-08-01

    Full Text Available OBJECTIVE: The aims of this study were to evaluate the tensile bond strengths between indirect composites and dentin of 3 recently developed self-adhesive resin cements and to determine mode of failure by SEM. MATERIAL AND METHODS: Exposed dentin surfaces of 70 mandibular third molars were used. Teeth were randomly divided into 7 groups: Group 1 (control group: direct composite resin restoration (Alert with etch-and-rinse adhesive system (Bond 1 primer/adhesive, Group 2: indirect composite restoration (Estenia luted with a resin cement (Cement-It combined with the same etch-and-rinse adhesive, Group 3: direct composite resin restoration with self-etch adhesive system (Nano-Bond, Group 4: indirect composite restoration luted with the resin cement combined with the same self-etch adhesive, Groups 5-7: indirect composite restoration luted with self-adhesive resin cements (RelyX Unicem, Maxcem, and Embrace WetBond, respectively onto the non-pretreated dentin surfaces. Tensile bond strengths of groups were tested with a universal testing machine at a constant speed of 1 mm/min using a 50 kgf load cell. Results were statistically analyzed by the Student's t-test. The failure modes of all groups were also evaluated. RESULTS: The indirect composite restorations luted with the self-adhesive resin cements (groups 5-7 showed better results compared to the other groups (p0.05. The surfaces of all debonded specimens showed evidence of both adhesive and cohesive failure. CONCLUSION: The new universal self-adhesive resins may be considered an alternative for luting indirect composite restorations onto non-pretreated dentin surfaces.

  10. The effect of organic solvents on one-bottle adhesives' bond strength to enamel and dentin.

    Science.gov (United States)

    Reis, André Figueiredo; Oliveira, Marcelo Tavares; Giannini, Marcelo; De Goes, Mário Fernando; Rueggeberg, Frederick A

    2003-01-01

    This study evaluated the microtensile bond strength (pTBS) of ethanol/water- and acetone-based, one-bottle adhesive systems to enamel (E) and dentin (D) in the presence (P) or absence (A) of their respective solvents. Thirty-two freshly extracted third molars were flattened with 600-grit SiC paper and restored with Single Bond (SB) or Prime&Bond 2.1 (PB) according to the manufacturers' instructions and after full solvent elimination. The molars were divided into eight test groups (n = 4): G1-SB-E-P, G2-SB-E-A, G3-PBE-P, G4-PB-E-A, G5-SB-D-P, G6-SB-D-A, G7-PB-D-P and G8-PB-D-A. After applying the adhesive resins, composite crowns of approximately 8 mm were built up with TPH Spectrum composite. After 24-hour water storage, the specimens were serially sectioned bucco-lingually to obtain 0.8 mm slabs that were trimmed to an hourglass shape, approximately 0.8 mm2 at the bonded interface. The specimens were tested in tension using a universal testing machine (0.5 mm/minute). The results were statistically analyzed by ANOVA and Tukey test. The frequency of fracture mode was compared using the Kruskal-Wallis test. There were no statistically significant differences in mean bond strength among the groups restored with or without solvent for enamel. However, the results were significantly different for the dentin groups (MPa): G5-26.2 +/- 8.6a; G7-23.6 +/- 11.3ab; G6-12.8 +/- 2.1bc; G8-6.2 +/- 3.1c. SEM examination indicated that the dentin group failure modes were significantly different from the enamel groups. The results suggest that the presence of organic solvents does not influence microTBS to enamel. However, microTBS to dentin was significantly affected by the absence of solvents in the adhesive system.

  11. Adhesion to pulp chamber dentin: Effect of ethanol-wet bonding technique and proanthocyanidins application

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    Pallavi Sharma

    2015-01-01

    Full Text Available Aim: To evaluate the microleakage of a simplified etch-and-rinse adhesive bonded to pulp chamber dentin with water-wet bonding (WWB or ethanol-wet bonding (EWB with and without proanthocyanidins (PA application. Materials and Methods: Total 88 non-carious extracted human molar teeth were sectioned horizontally to expose the pulp chambers 1.5 mm coronal to the cemento-enamel junction. After the pulp tissue extirpation, canal orifices were enlarged and the root ends were sealed. The samples were randomly divided equally into following four groups according to the four bonding techniques performed using Adper Single Bond 2 [SB] adhesive (1 WWB; (2 EWB; (3 WWB and PA application [WWB + PA]; (4 EWB and PA application [EWB + PA]. Composite resin restorations were performed in all the pulp chambers. Total 20 samples from each group were subjected to microleakage evaluation, and two samples per group were assessed under scanning electron microscope for interfacial micromorphology. Results: The least microleakage score was observed in group 2 (EWB with similar results seen in group 4 (EWB + PA (P = 0.918. Group 2 (EWB showed significantly less microleakage than group 1 (WWB; P = 0.002 and group 3 (WWB + PA; P = 0.009. Group 4 (EWB + PA also depicted significantly reduced microleakage as compared with group 1 (WWB; P = 0.001 and group 3 (WWB + PA; P = 0.003. Conclusion: The use of EWB technique in a clinically relevant simplified dehydration protocol significantly reduced microleakage in simplified etch-and-rinse adhesive, Adper Single Bond 2, bonded to pulp chamber dentin. Application of PA had no significant effect on the microleakage of the adhesive bonded with either WWB or EWB.

  12. Effect of simulated pulpal pressure on all-in-one adhesive bond strengths to dentine.

    Science.gov (United States)

    Hosaka, Keiichi; Nakajima, Masatoshi; Yamauti, Monica; Aksornmuang, Juthatip; Ikeda, Masaomi; Foxton, Richard M; Pashley, David H; Tagami, Junji

    2007-03-01

    To evaluate the durability of all-in-one adhesive systems bonded to dentine with and without simulated hydrostatic pulpal pressure (PP). Flat dentine surfaces of extracted human molars were prepared. Two all-in-one adhesive systems, One-Up Bond F (OBF) (Tokuyama Corp., Tokyo, Japan), and Fluoro Bond Shake One (FBS) (Shofu Co., Kyoto, Japan) were applied to the dentine surfaces under either a PP of 0 or 15cm H(2)O. Then, resin composite build-ups were made. The specimens bonded under pressure were stored in 37 degrees C water for 24h, 1 and 3 months under 15cm H(2)O PP. Specimens not bonded under pressure were stored under zero PP. After storage, the specimens were sectioned into slabs that were trimmed to hourglass shapes and subjected to micro-tensile bond testing (muTBS). The data were analysed using two-way ANOVA and Holm-Sidak HSD multiple comparison tests (alpha=0.05). The muTBS of OBF fell significantly (phydrostatic pressure storage fell significantly over the 3 months period, the decrease was less than half as much as specimens stored under PP. In FBS bonded specimens, although there was no significant difference between the muTBS with and without hydrostatic pulpal pressure at 24h, by 1 and 3 months of storage under PP, significant reductions were seen compared with the control group without PP. The muTBS of OBF bonded specimens was lowered more by simulated PP than by storage time; specimens bonded with FBS were not sensitive to storage time in the absence of PP, but showed lower bond strengths at 1 and 3 months in the presence of PP.

  13. Influence of different pre-etching times on fatigue strength of self-etch adhesives to dentin.

    Science.gov (United States)

    Takamizawa, Toshiki; Barkmeier, Wayne W; Tsujimoto, Akimasa; Suzuki, Takayuki; Scheidel, Donal D; Erickson, Robert L; Latta, Mark A; Miyazaki, Masashi

    2016-04-01

    The purpose of this study was to use shear bond strength (SBS) and shear fatigue strength (SFS) testing to determine the influence on dentin bonding of phosphoric acid pre-etching times before the application of self-etch adhesives. Two single-step self-etch universal adhesives [Prime & Bond Elect (EL) and Scotchbond Universal (SU)], a conventional single-step self-etch adhesive [G-aenial Bond (GB)], and a two-step self-etch adhesive [OptiBond XTR (OX)] were used. The SBS and SFS values were obtained with phosphoric acid pre-etching times of 3, 10, or 15 s before application of the adhesives, and for a control without pre-etching. For groups with 3 s of pre-etching, SU and EL showed higher SBS values than control groups. No significant difference was observed for GB among the 3 s, 10 s, and control groups, but the 15 s pre-etching group showed significantly lower SBS and SFS values than the control group. No significant difference was found for OX among the pre-etching groups. Reducing phosphoric acid pre-etching time can minimize the adverse effect on dentin bonding durability for the conventional self-etch adhesives. Furthermore, a short phosphoric acid pre-etching time enhances the dentin bonding performance of universal adhesives. © 2016 Eur J Oral Sci.

  14. The difference of tensile bond strength between total and self etch adhesive systems in dentin

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    Selly Yusalina

    2010-03-01

    Full Text Available Total etch adhesive system has been widely used in teeth conservation area as an adhesive agent before implicating composite resin restoration agent. The aim of this research is to prove the difference of tensile bond strength between total etch (Single Bond and self etch adhesive system (Adper prompt L-Pop on dentin surface in vitro. The extracted and non carries maxillary premolar teeth were used in this research and were divided into 2 groups. The first group comprised 15 specimen teeth etched in phosphoric acid and was applicated with the Single Bond adhesive agent. The second group comprised 15 specimen teeth, applicated with the Adper Prompt-L-Pop. The composite resin (Z 350, 3M was applied incrementally and each of the layers was rayed for 20 seconds. The specimens were stored in physiologic solution before they were tested. Tensile bond strength was measured by LRX Plus Lloyd Instrument, with 1 N load and 1 mm/minute speed, and the measurement result was in Mpa unit. The result was evaluated statistically by the Student t-test with α = 0.05. Single Bond (the 5th generation showed a better bond strength compared to the Adper Prompt-L-Pop (the 6th generation.

  15. Interfacial fracture of dentin adhesively bonded to quartz-fiber reinforced composite

    International Nuclear Information System (INIS)

    Melo, Renata M.; Rahbar, Nima; Soboyejo, Wole

    2011-01-01

    The paper presents the results of an experimental study of interfacial failure in a multilayered structure consisting of a dentin/resin cement/quartz-fiber reinforced composite (FRC). Slices of dentin close to the pulp chamber were sandwiched by two half-circle discs made of a quartz-fiber reinforced composite, bonded with bonding agent (All-bond 2, BISCO, Schaumburg) and resin cement (Duo-link, BISCO, Schaumburg) to make Brazil-nut sandwich specimens for interfacial toughness testing. Interfacial fracture toughness (strain energy release rate, G) was measured as a function of mode mixity by changing loading angles from 0 deg. to 15 deg. The interfacial fracture surfaces were then examined using Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray Spectroscopy (EDX) to determine the failure modes when loading angles changed. A computational model was also developed to calculate the driving forces, stress intensity factors and mode mixities. Interfacial toughness increased from ∼ 1.5 to 3.2 J/m 2 when the loading angle increases from ∼ 0 to 15 deg. The hybridized dentin/cement interface appeared to be tougher than the resin cement/quartz-fiber reinforced epoxy. The Brazil-nut sandwich specimen was a suitable method to investigate the mechanical integrity of dentin/cement/FRC interfaces.

  16. Evaluation of bond strength of self-etching adhesives having different pH on primary and permanent teeth dentin.

    Science.gov (United States)

    Ozmen, Bilal; Koyuturk, Alp Erdin; Tokay, Ugur; Cortcu, Murat; Sari, Mustafa Erhan

    2015-10-16

    The purpose of this in vitro study was to evaluate the dentin shear bond strength of 4 self-etching adhesives having a different pH on primary and permanent teeth dentin. The occlusal enamel was removed from 60 freshly extracted third molar and 60 primary second molar human teeth, which were randomly separated into 4 groups (n = 15). Four adhesive systems were applied: G-Bond (GC Corporation, Tokyo, Japan, pH: 1.5), Futura Bond M (Voco, Cuxhaver, Germany, pH: 1.4), Adper Prompt L-Pop (3M/ESPE, St Paul, MN, USA, pH: 0.8), and Clearfil S(3) Bond (Kuraray Medical, Tokyo, Japan, pH: 2.7) according to the manufacturer's instructions. After the application of dentin bonding agents, a composite resin material (Z250 Restorative A2, 3M ESPE, St. Paul, MN, USA) for permanent teeth and a compomer resin material (Dyract Extra A2, Dentsply, Konstanz, Germany) for primary teeth was applied onto the prepared dentin surfaces. The data were obtained by using a universal test machine at a crosshead speed of 1 mm/min. The mean values were compared using Tukey's multiple comparison test. Although there was no difference between adhesives on the permanent teeth, Clearfil S3 adhesive showed higher bond (18.07 ± 0.58 MPa) (P>0.05). Lower bond strength values were obtained from primary teeth and especially G-Bond adhesive (9.36 ± 0.48 MPa) (Padhesives with different pH and solvent types can be used successfully for permanent teeth dentin but adhesives with low pH did not provide greater shear bond strength values.

  17. Bond Strength of Resin Composite to Dentin with Different Adhesive Systems: Influence of Relative Humidity and Application Time.

    Science.gov (United States)

    Amsler, Fabienne; Peutzfeldt, Anne; Lussi, Adrian; Flury, Simon

    2015-06-01

    To investigate the influence of relative humidity and application time on bond strength to dentin of different classes of adhesive systems. A total of 360 extracted human molars were ground to mid-coronal dentin. The dentin specimens were treated with one of six adhesive systems (Syntac Classic, OptiBond FL, Clearfil SE Bond, AdheSE, Xeno Select, or Scotchbond Universal), and resin composite (Filtek Z250) was applied to the treated dentin surface under four experimental conditions (45% relative humidity/application time according to manufacturers' instructions; 45% relative humidity/reduced application time; 85% relative humidity/application time according to manufacturers' instructions; 85% relative humidity/reduced application time). After storage (37°C, 100% humidity, 24 h), shear bond strength (SBS) was measured and data analyzed with nonparametric ANOVA followed by Kruskal-Wallis tests and Mann-Whitney U-tests with Bonferroni-Holm correction for multiple testing (level of significance: α = 0.05). Increased relative humidity and reduced application time had no effect on SBS for Clearfil SE Bond and Scotchbond Universal (p = 1.00). For Syntac Classic, OptiBond FL, AdheSE, and Xeno Select there was no effect on SBS of reduced application time of the adhesive system (p ≥ 0.403). However, increased relative humidity significantly reduced SBS for Syntac Classic, OptiBond FL, and Xeno Select irrespective of application time (p ≤ 0.003), whereas for AdheSE, increased relative humidity significantly reduced SBS at recommended application time only (p = 0.002). Generally, increased relative humidity had a detrimental effect on SBS to dentin, but reduced application time had no effect.

  18. Effect of Numbers of Load Cycling on the Micro Tensile Bond Strength of Total Etch Adhesives to Dentin

    Directory of Open Access Journals (Sweden)

    AR Daneshkazemi

    2013-06-01

    Full Text Available Introduction: Today load cycling is used for similarity of invitro and invivo studies, though different results were reported in different studies. Therefore, this study aimed to investigate the effect of load cycling on micro tensile bond strength of two total etch adhesives to dentin. Methods: Enamel of 48 molar teeth were removed to expose the superficial dentin. The teeth were randomly divided into two equal groups, and were restored with Single bond (SB, ExciTE and Synergy composite. Then the teeth of each group were divided to 4 equal sub groups. Moreover, load cycling of 0, 50, 100, 200 k load cycle with 50 newton load was used. In each sub group, 12 hour glass slabs with 1mm2 thickness were made. Then the samples were loaded by Dartec testing machine (Model HC/10 with 1 mm/min cross head speed to make the fracture occur. Data were analyzed by ANOVA, t-test, Bonferroni tests. Results: The most micro tensile bond strength belonged to ExciTE without load cycling and lowest refered to SB with 200 k. There was a significant difference between the groups (p ExciTE= 0.0001, p SB = 0.001. Micro tensile bond strength in SB group was significantly lower than ExciTE (p= 0.001. Moreover, load cycling had negative effect on micro tensile bond strength. Conclusion: By increasing load cycling, micro tensile bond strength of both bondings decreased significantly

  19. EFFECT OF AN ADDITIONAL HYDROPHILIC VERSUS HYDROPHOBIC COAT ON THE QUALITY OF DENTINAL SEALING PROVIDED BY TWO-STEP ETCH-AND-RINSE ADHESIVES

    Science.gov (United States)

    Silva, Safira Marques de Andrade; Carrilho, Marcela Rocha de Oliveira; Marquezini, Luiz; Garcia, Fernanda Cristina Pimentel; Manso, Adriana Pigozzo; Alves, Marcelo Corrêa; de Carvalho, Ricardo Marins

    2009-01-01

    Objective: To test the hypothesis that the quality of the dentinal sealing provided by two-step etch-and-rinse adhesives cannot be altered by the addition of an extra layer of the respective adhesive or the application of a more hydrophobic, non-solvated resin. Material and Methods: full-crown preparations were acid-etched with phosphoric acid for 15 s and bonded with Adper Single Bond (3M ESPE), Excite DSC (Ivoclar/Vivadent) or Prime & Bond NT (Dentsply). The adhesives were used according to the manufacturers' instructions (control groups) or after application to dentin they were a) covered with an extra coat of each respective system or b) coated with a non-solvated bonding agent (Adper Scotchbond Multi-Purpose Adhesive, 3M ESPE). Fluid flow rate was measured before and after dentin surfaces were acid-etched and bonded with adhesives. Results: None of the adhesives or experimental treatments was capable to block completely the fluid transudation across the treated dentin. Application of an extra coat of the adhesive did not reduce the fluid flow rate of adhesive-bonded dentin (p>0.05). Conversely, the application of a more hydrophobic non-solvated resin resulted in significant reductions in the fluid flow rate (padhesives. Conclusions: The quality of the dentinal sealing provided by etch-and-rinse adhesives can be significantly improved by the application of a more hydrophobic, non-solvated bonding agent. PMID:19466248

  20. The effect of Er:YAG laser irradiation on the bond stability of self-etch adhesives at different dentin depths.

    Science.gov (United States)

    Karadas, Muhammet; Çağlar, İpek

    2017-07-01

    The aim of this study was to evaluate the effect of Er:YAG laser irradiation on the micro-shear bond strength of self-etch adhesives to the superficial dentin and the deep dentin before and after thermocycling. Superficial dentin and deep dentin surfaces were prepared by flattening of the occlusal surfaces of extracted human third molars. The deep or superficial dentin specimens were randomized into three groups according to the following surface treatments: group I (control group), group II (Er:YAG laser; 1.2 W), and group III (Er:YAG laser; 0.5 W). Clearfil SE Bond or Clearfil S 3 Bond was applied to each group's dentin surfaces. After construction of the composite blocks on the dentin surface, the micro-shear bond testing of each adhesive was performed at 24 h or after 15,000 thermal cycles. The data were analyzed using a univariate analysis of variance and Tukey's test (p  0.05). However, deep-dentin specimens irradiated with laser showed significantly higher bond strengths than did control specimens after thermocycling (p adhesives may be altered by the dentin depth. Regardless of the applied surface treatment, deep dentin showed significant bond degradation.

  1. Experimental etch-and-rinse adhesive systems containing MMP-inhibitors: Physicochemical characterization and resin-dentin bonding stability.

    Science.gov (United States)

    da Silva, Eduardo Moreira; de Sá Rodrigues, Carolina Ullmann Fernandes; de Oliveira Matos, Marcos Paulo; de Carvalho, Thais Rodrigues; dos Santos, Glauco Botelho; Amaral, Cristiane Mariote

    2015-12-01

    To evaluate the degree of conversion (DC%), water sorption (WS), solubility (SO) and the resin-dentin bonding stability (μTBS) of experimental (EXP) etch-and-rinse adhesive systems containing MMP-inhibitors: Galardin-GAL, Batimastat-BAT, GM1489-GM1 and chlorhexidine diacetate-CHX. DC% was measured using FT-IR spectroscopy, while WS and SO were calculated based on ISO4049. Thirty-six human molars were wet ground until the occlusal dentin was exposed. The adhesive systems were applied and resin composite buildups were incrementally constructed. After 24 h immersion in distilled water at 37 °C, the specimens were cut into resin-dentin beams with a cross-sectional area of 1 mm(2). The μTBS was evaluated after 24 h, 6 months and 12 months of water storage at 37 °C. Adper Single Bond 2 (SB2) was used as a commercial control. The data were analyzed using ANOVA and Tukey's HSD test. SB2 presented the highest DC% (p0.05). SO was found to be not significant (p>0.05). All adhesive systems maintained μTBS stability after 6 months of water storage. Only BAT, GM1 and CHX maintained μTBs stability after 12 months of water storage. The experimental adhesive systems with GM1489 and chlorhexidine diacetate presented the best physicochemical properties and preserved resin-dentin bonding stability after 12 months of water storage. GM1489 could be suitable for inclusion as an MMP-inhibitor in etch-and-rinse adhesive systems to maintain resin-dentin bonding stability over time. Copyright © 2015. Published by Elsevier Ltd.

  2. Effects of type I collagen degradation on the durability of three adhesive systems in the early phase of dentin bonding.

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    Lin Hu

    Full Text Available This study was designed to evaluate the effects of type I collagen degradation on the durability of three adhesive systems in the early phase of dentin bonding.Bonded dentin specimens were prepared using three different types of adhesive systems. Micro-tensile bond strength and degradation of collagen were tested before, and after 1 month or 4 months of aging in artificial saliva. The relationship between micro-tensile bond strength and collagen degradation was analyzed by calculating their Pearson's correlation coefficient.Aging induced time-dependent reduction in micro-tensile bond strengths for all the tested adhesive systems, although such reduction for the single-step self-etching adhesive G-Bond (GB was not statistically significant. The bond strength of the two-step self-etching primer adhesive system Clearfil SE Bond (SEB was similar to that of the two-step etch-and-rinse self-priming adhesive system Single Bond 2 (SB, and they were both significantly reduced after one or four months of aging. A negative correlation was found between the degree of collagen degradation and magnitude of micro-tensile bond strength (r = -0.65, p = 0.003. The Pearson's correlation coefficient was 0.426, indicating that 42.6% of the aging-induced reduction in bond strength can be explained by the degradation of collagen.In the early phase of dentin bonding, there was a negative correlation between the degree of collagen degradation and the magnitude of micro-tensile bond strength. The reduction of bond strength was accompanied by the degradation of collagen. These results provide evidence for the causative relationship between the degradation of collagen and the deterioration of dentin-adhesive interface.

  3. [Adhesion of sealer cements to dentin with and without smear layer].

    Science.gov (United States)

    Gettleman, B H; Messer, H H; ElDeeb, M E

    1991-01-01

    The influence of a smear layer on the adhesion of sealer cements to dentin was assessed in recently extracted human anterior teeth. A total of 120 samples was tested, 40 per sealer; 20 each with and without the smear layer. The teeth were split longitudinally, and the internal surfaces were ground flat. One-half of each tooth was left with the smear layer intact, while the other half had the smear removed by washing for 3 min with 17% EDTA followed by 5.25% NaOCI. Evidence of the ability to remove the smear layer was verified by scanning electron microscopy. Using a specially designed jig, the sealer was placed into a 4-mm wide x 4 mm deep well which was then set onto the tooth.

  4. Effect of Self-Adhesive and Separate Etch Adhesive Dual Cure Resin Cements on the Bond Strength of Fiber Post to Dentin at Different Parts of the Root

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    Ehsan Mohamadian Amiri

    2017-10-01

    Full Text Available Objectives: Bonding of fiber posts to intracanal dentin is challenging in the clinical setting. This study aimed to compare the effect of self-adhesive and separate etch adhesive dual cure resin cements on the bond strength of fiber post to dentin at different parts of the root.Materials and Methods: This in-vitro experimental study was conducted on 20 single-rooted premolars. The teeth were decoronated at 1mm coronal to the cementoenamel junction (CEJ, and the roots underwent root canal treatment. Post space was prepared in the roots. Afterwards, the samples were randomly divided into two groups. In group 1, the fiber posts were cemented using Rely X Unicem cement, while in group 2, the fiber posts were cemented using Duo-Link cement, according to the manufacturer's instructions. The intracanal post in each root was sectioned into three segments of coronal, middle, and apical, and each cross-section was subjected to push-out bond strength test at a crosshead speed of 1mm/minute until failure. Push-out bond strength data were analyzed using independent t-test and repeated measures ANOVA.Results: The bond strength at the middle and coronal segments in separate etch adhesive cement group was higher than that in self-adhesive cement group. However, the bond strength at the apical segment was higher in self-adhesive cement group compared to that in the other group. Overall, the bond strength in separate etch adhesive cement group was significantly higher than that in self-adhesive cement group (P<0.001.Conclusions: Bond strength of fiber post to intracanal dentin is higher after the use of separate etch adhesive cement compared to self-adhesive cement.

  5. BOND STRENGTH DURABILITY OF SELF-ETCHING ADHESIVES AND RESIN CEMENTS TO DENTIN

    Science.gov (United States)

    Chaves, Carolina de Andrade Lima; de Melo, Renata Marques; Passos, Sheila Pestana; Camargo, Fernanda Pelógia; Bottino, Marco Antonio; Balducci, Ivan

    2009-01-01

    Objectives: To evaluate the microtensile bond strength (μTBS) of one- (Xeno III, Dentsply) and two-step (Tyrian-One Step Plus, Bisco) self-etching adhesive systems bonded to dentin and cemented to chemically cured (C&B Metabond) or light-cured paste of a dual-cure resin cement (Variolink II, Ivoclar) within a short (24 h) and long period of evaluation (90 days). Material and Methods: Forty recently extracted human molars had their roots removed and their occlusal dentin exposed and ground wet with 600-grit SiC paper. After application of one of the adhesives, the resin cement was applied to the bonded surface and a composite resin block was incrementally built up to a height of 5 mm (n=10). The restored teeth were stored in distilled water at 37°C for 7 days. The teeth were then cut along two axes (x and y), producing beam-shaped specimens with 0.8 mm2 cross-sectional area, which were subjected to μTBS testing at a crosshead speed of 0.05 mm/min and stressed to failure after 24 h or 90 days of storage in water. The μTBS data in MPa were subjected to three-way analysis of variance and Tukey's test (α= 0.05). Results: The interaction effect for all three factors was statistically significant (three-way ANOVA, padhesive combination that provided the most promising bond strength after 90 days of storage in water. PMID:19466243

  6. Effect of dentin dehydration and composite resin polymerization mode on bond strength of two self-etch adhesives

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    Pooran Samimi

    2016-01-01

    Full Text Available Background: Dual-cured composite resins are similar to self-cured composite resins in some of their clinical applications due to inadequate irradiation, lack of irradiation, or delayed irradiation. Therefore, incompatibility with self-etch adhesives (SEAs should be taken into account with their use. On the other, the extent of dentin dehydration has a great role in the quality of adhesion of these resin materials to dentin. The aim of this study was to investigate the effect of dentin dehydration and composite resin polymerization mode on bond strength of two SEAs. Materials and Methods: A total of 120 dentinal specimens were prepared from extracted intact third molars. Half of the samples were dehydrated in ethanol with increasing concentrations. Then Clearfil SE Bond (CSEB and Prompt L-Pop (PLP adhesives were applied in the two groups. Cylindrical composite resin specimens were cured using three polymerization modes: (1 Immediate light-curing, (2 delayed light-curing after 20 min, and (3 self-curing. Bond strength was measured using universal testing machine at a crosshead speed of 1 mm/min. Data were analyzed with two-way ANOVA and Duncan post hoc tests. Statistical significance was defined at P 0.05. PLP showed significant differences between subgroups with the lowest bond strength in hydrated dentin with delayed light-curing and self-cured mode of polymerization. Conclusion: Within the limitations of this study, a delay in composite resin light-curing or using chemically cured composite resin had a deleterious effect on dentin bond strength of single-step SEAs used in the study.

  7. A micromechanics model of the elastic properties of human dentine

    Energy Technology Data Exchange (ETDEWEB)

    Kinney, J. H. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Balooch, M. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Marshall, G. W. [Univ. of California, San Francisco, CA (United States). Dept. of Restorative Dentistry; Marshall, S. J. [Univ. of California, San Francisco, CA (United States). Dept. of Restorative Dentistry

    1999-10-01

    A generalized self-consistent model of cylindrical inclusions in a homogeneous and isotropic matrix phase was used to study the effects of tubule orientation on the elastic properties of dentin. Closed form expressions for the five independent elastic constants of dentin were derived in terms of tubule concentration, and the Young's moduli and Poisson ratios of peri- and intertubular dentin. An atomic force microscope (AFM) indentation technique determined the Young's moduli of the peri- and intertubular dentin as approximately 30 GPa and 15 GPa, respectively. Over the natural variation in tubule density found in dentin, there was only a slight variation in the axial and transverse shear moduli with position in the tooth, and there was no measurable effect of tubule orientation. We conclude that tubule orientation has no appreciable effect on the elastic behavior of normal dentin, and that the elastic properties of healthy dentin can be modeled as an isotropic continuum with a Young's modulus of approximately 16 GPa and a shear modulus of 6.2 GPa.

  8. Influence of CVD diamond tips and Er:YAG laser irradiation on bonding of different adhesive systems to dentin

    OpenAIRE

    da Silva, Melissa Aline [UNESP; Nicolo, Rebeca Di [UNESP; Barcellos, Daphne Camara [UNESP; Batista, Graziela Ribeiro [UNESP; Pucci, Cesar Rogerio [UNESP; Torres, Carlos Rocha Gomes [UNESP; Borges, Alessandra Bühler [UNESP

    2013-01-01

    Aim: The aim of this study was to compare the microtensile bond strength of three adhesive systems, using different methods of dentin preparation. Materials and methods: A hundred and eight bovine teeth were used. The dentin from buccal face was exposed and prepared with three different methods, divided in 3 groups: Group 1 (DT)- diamond tip on a high-speed handpiece; Group 2 (CVD)-CVD tip on a ultrasonic handpiece; Group 3 (LA)-Er: YAG laser. The teeth were divided into 3 subgroups, accordin...

  9. The shear bond strength of self-adhesive resin cements to dentin and enamel: an in vitro study.

    Science.gov (United States)

    Rodrigues, Raphaela F; Ramos, Carla M; Francisconi, Paulo A S; Borges, Ana Flávia S

    2015-03-01

    Clinicians continue to search for ways to simplify bonding procedures without compromising clinical efficacy. The purpose of this study was to evaluate the shear strength of self-adhesive cements RelyX U100 and RelyX U200, and conventional resin cement RelyX ARC to enamel and dentin after different surface treatments. The crowns of 120 bovine incisor teeth were separated from the roots and embedded in epoxy resin in polyvinyl chloride tubes. In each tooth, the area to be cemented was delimited with central holed adhesive tape. The teeth were distributed into 12 groups (n=10) according to the substrate; etched or not with 37% phosphoric acid; and cement type of enamel-U100, enamel-phosphoric acid-U100, enamel-U200, enamel-phosphoric acid-U200, enamel-ARC, enamel-phosphoric acid-ARC, dentin-U100, dentin-phosphoric acid-U100, dentin-U200, dentin-phosphoric acid-U200, dentin-ARC, and dentin-phosphoric acid-ARC. After 7 days of storage in artificial saliva, shear strength tests were performed by using a universal testing machine (0.5 mm/min). The data were analyzed with 3-way ANOVA and the Tukey test (α=.05). Fracture analysis was performed with a light microscope. Two specimens from each group were analyzed with a scanning electron microscope. In enamel, ARC (9.96 MPa) had higher shear strength (P=.038) than U100 (5.14 MPa); however, after surface etching, U100 (17.81 MPa) and U200 (17.52 MPa) had higher shear strength (Padhesive type. U200 self-adhesive cement had similar bond strength to the ARC in enamel, but the combination with phosphoric acid had the best bond strength. For dentin, self-adhesive resin cements are equally effective alternatives to conventional resin cement. Copyright © 2015 Editorial Council for the Journal of Prosthetic Dentistry. Published by Elsevier Inc. All rights reserved.

  10. Shear bond strength of one-step self-etch adhesives to dentin : evaluation of NaOCl pretreatment

    OpenAIRE

    Colombo, Marco; Beltrami, Riccardo; Chiesa, Marco; Poggio, Claudio; Scribante, Andrea

    2018-01-01

    The aim of this study was to evaluate the influence of dentin pretreatment with NaOCl on shear bond strength of four one-step self-etch adhesives with different pH values. Bovine permanent incisors were used. Four one-step self-etch adhesives were tested: Adper? Easy Bond, Futurabond NR, G-aenial Bond, Clearfil S3 Bond. One two-step self-etch adhesive (Clearfil SE Bond) was used as control. Group 1- no pretreatment; group 2- pretratment with 5,25 % NaOCl; group 3- pretreatment with 37 % H3PO4...

  11. Effect of air-drying time of single-application self-etch adhesives on dentin bond strength.

    Science.gov (United States)

    Chiba, Yasushi; Yamaguchi, Kanako; Miyazaki, Masashi; Tsubota, Keishi; Takamizawa, Toshiki; Moore, B Keith

    2006-01-01

    This study examined the effect of air-drying time of adhesives on the dentin bond strength of several single-application self-etch adhesive systems. The adhesive/resin composite combinations used were: Adper Prompt L-Pop/Filtek Z250 (AP), Clearfil Tri-S Bond/Clearfil AP-X (CT), Fluoro Bond Shake One/Beautifil (FB), G-Bond/Gradia Direct (GB) and One-Up Bond F Plus/Palfique Estelite (OF). Bovine mandibular incisors were mounted in self-curing resin and wet ground with #600 SiC to expose labial dentin. Adhesives were applied according to each manufacturer's instructions followed by air-drying time for 0 (without air-drying), 5 and 10 seconds. After light irradiation of the adhesives, the resin composites were condensed into a mold (phi4x2 mm) and polymerized. Ten samples per test group were stored in 37 degrees C distilled water for 24 hours; they were then shear tested at a crosshead speed of 1.0 mm/minute. One-way ANOVA followed by Tukey's HSD tests (alpha = 0.05) were done. FE-SEM observations of the resin/dentin interface were also conducted. Dentin bond strength varied with the different air drying times and ranged from 5.8 +/- 2.4 to 13.9 +/- 2.8 MPa for AP, 4.9 +/- 1.5 to 17.1 +/- 2.3 MPa for CT, 7.9 +/- 2.8 to 13.8 +/- 2.4 MPa for FB, 3.7 +/- 1.4 to 13.4 +/- 1.2 MPa for GB and 4.6 +/- 2.1 to 13.7 +/- 2.6 MPa for OF. With longer air drying of adhesives, no significant changes in bond strengths were found for the systems used except for OF. Significantly lower bond strengths were obtained for the 10-second air-drying group for OF. From FE-SEM observations, gaps between the cured adhesive and resin composites were observed for the specimens without the air drying of adhesives except for OF. The data suggests that, with four of the single-application self-etch adhesive systems, air drying is essential to obtain adequate dentin bond strengths, but increased drying time does not significantly influence bond strength. For the other system studied, the bond strength

  12. Shear bond strength of porcelain laminate veneers to enamel, dentine and enamel-dentine complex bonded with different adhesive luting systems.

    Science.gov (United States)

    Öztürk, Elif; Bolay, Şükran; Hickel, Reinhard; Ilie, Nicoleta

    2013-02-01

    The aim of this study was to evaluate the shear bond strength of porcelain laminate veneers to 3 different surfaces by means of enamel, dentine, and enamel-dentine complex. One hundred thirty-five extracted human maxillary central teeth were used, and the teeth were randomly divided into 9 groups (n=15). The teeth were prepared with 3 different levels for bonding surfaces of enamel (E), dentine (D), and enamel-dentine complex (E-D). Porcelain discs (IPS e.max Press, Ivoclar Vivadent) of 2mm in thickness and 4mm in diameter were luted to the tooth surfaces by using 2 light-curing (RelyX Veneer [RV], 3M ESPE; Variolink Veneer [VV], Ivoclar Vivadent) and a dual-curing (Variolink II [V2], Ivoclar Vivadent) adhesive systems according to the manufacturers' instructions. Shear bond strength test was performed in a universal testing machine at 0.5mm/min until bonding failure. Failure modes were determined under a stereomicroscope, and fracture surfaces were evaluated with a scanning electron microscope. The data were statistically analysed (SPSS 17.0) (p=0.05). Group RV-D exhibited the lowest bond strength value (5.42±6.6MPa). There was statistically no difference among RV-D, V2-D (13.78±8.8MPa) and VV-D (13.84±6.2MPa) groups (p>0.05). Group VV-E exhibited the highest bond strength value (24.76±8.8MPa). The type of tooth structure affected the shear bond strength of the porcelain laminate veneers to the 3 different types of tooth structures (enamel, dentine, and enamel-dentine complex). When dentine exposure is necessary during preparation, enough sound enamel must be protected as much as possible to maintain a good bonding; to obtain maximum bond strength, preparation margins should be on sound enamel. Copyright © 2012 Elsevier Ltd. All rights reserved.

  13. [The influence of the chemo-mechanical removal of the smear-layer and the use of a dentin adhesive on microleakage of composite resin restorations].

    Science.gov (United States)

    de la Macorra García, J C; Gómez Martínez, A; Gutiérrez Argumosa, B

    1989-02-01

    We present an "in vitro" study of microfiltration in composite resin restorations with a perimetral seal placed totally in cement. We compare the sealing capability of a dentin adhesive (ScotchBond I) used in two ways: habitual, without conditioning dentin and conditioning it by means of the Caridex system. This produced no increasing of sealing capability under the study conditions.

  14. Bond strength durability of self-etching adhesives and resin cements to dentin

    Directory of Open Access Journals (Sweden)

    Carolina de Andrade Lima Chaves

    2009-06-01

    Full Text Available OBJECTIVES: To evaluate the microtensile bond strength (µTBS of one- (Xeno III, Dentsply and two-step (Tyrian-One Step Plus, Bisco self-etching adhesive systems bonded to dentin and cemented to chemically cured (C&B Metabond or light-cured paste of a dual-cure resin cement (Variolink II, Ivoclar within a short (24 h and long period of evaluation (90 days. MATERIAL AND METHODS: Forty recently extracted human molars had their roots removed and their occlusal dentin exposed and ground wet with 600-grit SiC paper. After application of one of the adhesives, the resin cement was applied to the bonded surface and a composite resin block was incrementally built up to a height of 5 mm (n=10. The restored teeth were stored in distilled water at 37ºC for 7 days. The teeth were then cut along two axes (x and y, producing beam-shaped specimens with 0.8 mm² cross-sectional area, which were subjected to µTBS testing at a crosshead speed of 0.05 mm/min and stressed to failure after 24 h or 90 days of storage in water. The µTBS data in MPa were subjected to three-way analysis of variance and Tukey's test (α= 0.05. RESULTS: The interaction effect for all three factors was statistically significant (three-way ANOVA, p<0.001. All eight experimental means (MPa were compared by the Tukey's test (p<0.05 and the following results were obtained: Tyrian-One Step Plus /C&B/24 h (22.4±7.3; Tyrian-One Step Plus /Variolink II/24 h (39.4±11.6; Xeno III/C&B/24 h (40.3±12.9; Xeno III/Variolink II/24 h (25.8±10.5; Tyrian-One Step Plus /C&B/90 d (22.1±12.8 Tyrian-One Step Plus/VariolinkII/90 d (24.2±14.2; Xeno III/C&B/90 d (27.0±13.5; Xeno III/Variolink II/90 d (33.0±8.9. CONCLUSIONS: Xeno III/Variolink II was the luting agent/adhesive combination that provided the most promising bond strength after 90 days of storage in water.

  15. Effect of Re-Application of Microbrush on Micro Tensile Bond Strength of an Adhesive to Dentin

    Directory of Open Access Journals (Sweden)

    Seied Majid Mosavi Nasab

    2013-02-01

    Full Text Available Background and Aims: Re-application of microbrush may affect the micro tensile bond strength of adhesives to dentin. The aim of this study was to evaluate the effect of re-application of microbrushes on the micro tensile bond strength of an adhesive to dentin.Materials and Methods: Thirty freshly extracted molars teeth were collected and enamel of occlusal surface were removed to expose superficial dentin. Then superficial dentin was etched, washed and partially air dried.According to the times of application of microbrush, teeth were divided into two test groups. In group 1, newmicrobrushs were used, but in group 2, the ones that were already used for twice were included. Ambar dentin bonding agent (FGM/Brazil was applied to the etched dentin with microbrushes according to the manufacturer’s instructions. Then the crown of teeth was built up with LLiss (FGM/Brazil composite resin. The teeth were sectioned in buccolingual direction to obtain 1mm slabs. Then 50 hourglass- shape samples were made from 30 teeth (25 Specimens per group. The microtensile bond strength of the specimens was tested using MTD500 (SD Mechatronik, Germany. The data were statistically analyzed by T-test.Results: The mean values for the microtensile bond strength were 30.49±7.18 and 23.61±9.06 MPa±SD for the first and second groups, respectively. There was significant difference between the groups (P=0.005.Conclusion: Microbrushes should not be used for more than one cavity preparation.

  16. Bond strength of composite to dentin: effect of acid etching and laser irradiation through an uncured self-etch adhesive system

    International Nuclear Information System (INIS)

    Castro, F L A; Carvalho, J G; Andrade, M F; Saad, J R C; Hebling, J; Lizarelli, R F Z

    2014-01-01

    This study evaluated the effect on micro-tensile bond strength (µ-TBS) of laser irradiation of etched/unetched dentin through an uncured self-etching adhesive. Dentinal surfaces were treated with Clearfil SE Bond Adhesive (CSE) either according to the manufacturer’s instructions (CSE) or without applying the primer (CSE/NP). The dentin was irradiated through the uncured adhesive, using an Nd:YAG laser at 0.75 or 1 W power settings. The adhesive was cured, composite crowns were built up, and the teeth were sectioned into beams (0.49 mm 2 ) to be stressed under tension. Data were analyzed using one-way ANOVA and Tukey statistics (α = 5%). Dentin of the fractured specimens and the interfaces of untested beams were observed under scanning electron microscopy (SEM). The results showed that non-etched irradiated surfaces presented higher µ-TBS than etched and irradiated surfaces (p < 0.05). Laser irradiation alone did not lead to differences in µ-TBS (p > 0.05). SEM showed solidification globules on the surfaces of the specimens. The interfaces were similar on irradiated and non-irradiated surfaces. Laser irradiation of dentin through the uncured adhesive did not lead to higher µ-TBS when compared to the suggested manufacturer’s technique. However, this treatment brought benefits when performed on unetched dentin, since bond strengths were higher when compared to etched dentin. (paper)

  17. Effect of different surface treatments on adhesion of In-Ceram Zirconia to enamel and dentin substrates.

    Science.gov (United States)

    Saker, Samah; Ibrahim, Fatma; Ozcan, Mutlu

    2013-08-01

    Resin bonding of In-Ceram Zirconia (ICZ) ceramics is still a challenge, especially for minimally invasive applications. This study evaluated the adhesion of ICZ to enamel and dentin after different surface treatments of the ceramic. ICZ ceramic specimens (diameter: 6 mm; thickness: 2 mm) (N = 100) were fabricated following the manufacturer's instructions and randomly assigned to 5 groups (n = 20), according to the surface treatment methods applied. The groups were as follows: group C: no treatment; group SB: sandblasting; group SCS-S: CoJet+silane; group SCS-P: CoJet+Alloy Primer; group GE-S: glaze+ hydrofluoric acid etching (9.6%) for 60 s+silane. Each group was randomly divided into two subgroups to be bonded to either enamel or dentin (n = 10 per group) using MDP-based resin cement (Panavia F2.0). All the specimens were subjected to thermocycling (5000x, 5°C-55°C). The specimens were mounted in a universal testing machine and tensile force was applied to the ceramic/cement interface until failure occurred (1 mm/min). After evaluating all the debonded specimens under SEM, the failure types were defined as either "adhesive" with no cement left on the ceramic surface (score 0) or "mixed" with less than 1/2 of the cement left adhered to the surface with no cohesive failure of the substrate (score 1). The data were statistically evaluated using 2-way ANOVA and Tukey's tests (α = 0.05). The highest tensile bond strength for the enamel surfaces was obtained in group GE-S (18.1 ± 2 MPa) and the lowest in group SB (7.1 ± 1.4 MPa). Regarding dentin, group CSC-P showed the highest (12 ± 1.3 MPa) and SB the lowest tensile bond strength (5.7 ± 0.4 MPa). Groups SB, CSC-S, CSC-P, and GE-S did not show significant differences between the different surface treatments on either enamel or dentin surfaces (p enamel and dentin substrates (p enamel substrates, exclusively adhesive failures from ICZ (score 0) were found, on dentin exclusively mixed failures were observed (score

  18. Effect of surface treatments on the flexural properties and adhesion of glass fiber-reinforced composite post to self-adhesive luting agent and radicular dentin.

    Science.gov (United States)

    Elnaghy, Amr M; Elsaka, Shaymaa E

    2016-01-01

    This study evaluated the effect of different surface treatments on the flexural properties and adhesion of glass fiber post to self-adhesive luting agent and radicular dentin. Seventy-five single-rooted human teeth were prepared to receive a glass fiber post (Reblida). The posts were divided into five groups according to the surface treatment: Gr C (control; no treatment), Gr S (silanization for 60 s), Gr AP (airborne-particle abrasion), Gr HF (etching with 9 % hydrofluoric acid for 1 min), and Gr M10 (etching with CH2Cl2 for 10 min). Dual-cure self-adhesive luting agent (Rely X Unicem) was applied to each group for testing the adhesion using micropush-out test. Failure types were examined with stereomicroscope and surface morphology of the posts was characterized using a scanning electron microscopy (SEM). Flexural properties of posts were assessed using a three-point bending test. Data were analyzed using ANOVA and Tukey's HSD test. Statistical significance was set at the 0.05 probability level. Groups treated with M10 showed significantly higher bond strength than those obtained with other surface treatments (P C > S > AP > HF. Most failure modes were adhesive type of failures between dentin and luting agent (48.2%). SEM analysis revealed that the fiber post surfaces were modified after surface treatments. The surface treatments did not compromise the flexural properties of fiber posts. Application of M10 to the fiber post surfaces enhanced the adhesion to self-adhesive luting agent and radicular dentin.

  19. Influence of CVD diamond tips and Er:YAG laser irradiation on bonding of different adhesive systems to dentin.

    Science.gov (United States)

    da Silva, Melissa Aline; Di Nicolo, Rebeca; Barcellos, Daphne Camara; Batista, Graziela Ribeiro; Pucci, Cesar Rogerio; Rocha Gomes Torres, Carlos; Borges, Alessandra Bühler

    2013-01-01

    The aim of this study was to compare the microtensile bond strength of three adhesive systems, using different methods of dentin preparation. A hundred and eight bovine teeth were used. The dentin from buccal face was exposed and prepared with three different methods, divided in 3 groups: Group 1 (DT)- diamond tip on a high-speed handpiece; Group 2 (CVD)-CVD tip on a ultrasonic handpiece; Group 3 (LA)-Er: YAG laser. The teeth were divided into 3 subgroups, according adhesive systems used: Subgroup 1-Adper Single Bond Plus/3M ESPE (SB) total-etch adhesive; Subgroup 2-Adper Scotchbond SE/3M ESPE (AS) selfetching adhesive; Subgroup 3-Clearfil SE Bond/Kuraray (CS) selfetching adhesive. Blocks of composite (Filtek Z250-3M ESPE) 4 mm high were built up and specimens were stored in deionized water for 24 hours at 37°C. Serial mesiodistal and buccolingual cuts were made and stick-like specimens were obtained, with transversal section of 1.0 mm(2). The samples were submitted to microtensile test at 1 mm/min and load of 10 kg in a universal testing machine. Data (MPa) were subjected to ANOVA and Tukey's tests (p adhesive produced significantly lower bond strength values compared to other groups. Surface treatment with Er: YAG laser associated with Single Bond Plus or Clearfil SE Bond adhesives and surface treatment with CVD tip associated with Adper Scotchbond SE adhesive produced significantly lower bond strength values compared to surface treatment with diamond or CVD tips associated with Single Bond Plus or Adper Scotchbond SE adhesives. Interactions between laser and the CVD tip technologies and the different adhesive systems can produce a satisfactory bonding strength result, so that these associations may be beneficial and enhance the clinical outcomes.

  20. Sealing of adhesive systems in ferric sulfate-contaminated dentinal margins in class V composite resin restorations

    Directory of Open Access Journals (Sweden)

    Niloofar Shadman

    2016-03-01

    Full Text Available Background. Hemostatic agents are applied to prepare an isolated bleeding-free condition during dental treatments and can influence adhesive restorations. This study evaluated the effect of a hemostatic agent (ViscoStat on microleakage of contaminated dentinal margin of class V composite resin restorations with three adhesives. Methods. Sixty freshly extracted human molars were selected and class V cavities (3×3×1.5 mm were prepared on buccal and lingual surfaces. Gingival margins of the cavities were placed below the cementoenamel junction. The teeth were divided into six groups randomly. The adhesives were Excite, AdheSE and AdheSE One. In three groups, the gingival walls of the cavities were contaminated with ViscoStat and then rinsed. The cavities were restored with composite resin and light-cured. After storage in distilled water (37°C for 24 hours and polishing, the samples were thermocycled and sealed with nail varnish. Then they were stored in 1% basic fuchsin for 24 hours, rinsed and mounted in self-cured acryl resin, followed by sectioning buccolingually. Dye penetration was observed under a stereomicroscope and scored. Data were statistically analyzed with Kruskal-Wallis and Mann-Whitney U tests. P 0.05. In the contaminated groups, Excite had significantly less microleakage than the others (P = 0.003. AdheSE and AdheSE One did not exhibit significant difference in microleakage (P > 0.05. Conclusion. ViscoStat hemostatic agent increased dentinal microleakage in AdheSE and AdheSE One adhesives with no effect on Excite.

  1. Micro-CT evaluation of internal adaptation in resin fillings with different dentin adhesives

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    Seung-Hoon Han

    2014-02-01

    Full Text Available Objectives The purpose of present study was to evaluate the internal adaptation of composite restorations using different adhesive systems. Materials and Methods Typical class I cavities were prepared in 32 human third molars. The teeth were divided into the following four groups: 3-step etch-and-rinse, 2-step etch-and-rinse, 2-step self-etch and 1-step self-etch system were used. After the dentin adhesives were applied, composite resins were filled and light-cured in two layers. Then, silver nitrate solution was infiltrated, and all of the samples were scanned by micro-CT before and after thermo-mechanical load cycling. For each image, the length to which silver nitrate infiltrated, as a percentage of the whole pulpal floor length, was calculated (%SP. To evaluate the internal adaptation using conventional method, the samples were cut into 3 pieces by two sectioning at an interval of 1 mm in the middle of the cavity and they were dyed with Rhodamine-B. The cross sections of the specimens were examined by stereomicroscope. The lengths of the parts where actual leakage was shown were measured and calculated as a percentage of real leakage (%RP. The values for %SP and %RP were compared. Results After thermo-mechanical loading, all specimens showed significantly increased %SP compared to before thermo-mechanical loading and 1-step self-etch system had the highest %SP (p < 0.05. There was a tendency for %SP and %RP to show similar microleakage percentage depending on its sectioning. Conclusions After thermo-mechanical load cycling, there were differences in internal adaptation among the groups using different adhesive systems.

  2. Effect of air-blowing duration on the bond strength of current one-step adhesives to dentin.

    Science.gov (United States)

    Fu, Jiale; Saikaew, Pipop; Kawano, Shimpei; Carvalho, Ricardo M; Hannig, Matthias; Sano, Hidehiko; Selimovic, Denis

    2017-08-01

    To evaluate the influence of different air-blowing durations on the micro-tensile bond strength (μTBS) of five current one-step adhesive systems to dentin. One hundred and five caries-free human molars and five current one-step adhesive systems were used: ABU (All Bond Universal, Bisco, Inc.), CUB (CLEARFIL™ Universal Bond, Kuraray), GPB (G-Premio BOND, GC), OBA (OptiBond All-in-one, Kerr) and SBU (Scotchbond Universal, 3M ESPE). The adhesives were applied to 600 SiC paper-flat dentin surfaces according to each manufacturer's instructions and were air-dried with standard, oil-free air pressure of 0.25MPa for either 0s, 5s, 15s or 30s before light-curing. Bond strength to dentin was determined by using μTBS test after 24h of water storage. The fracture pattern on the dentin surface was analyzed by SEM. The resin-dentin interface of untested specimens was visualized by panoramic SEM image. Data from μTBS were analyzed using two-way ANOVA (adhesive vs. air-blowing time), and Games-Howell (a=0.05). Two-way ANOVA revealed a significant effect of materials (p=0.000) and air-blowing time (p=0.000) on bond strength to dentin. The interaction between factors was also significantly different (p=0.000). Maximum bond strength for each system were recorded, OBA/15s (76.34±19.15MPa), SBU/15s (75.18±12.83MPa), CUB/15s (68.23±16.36MPa), GPB/30s (55.82±12.99MPa) and ABU/15s (44.75±8.95MPa). The maximum bond strength of OBA and SUB were significantly higher than that of GPB and ABU (padhesive systems is material-dependent (p=0.000), and was influenced by air-blowing duration (p=0.000). For the current one-step adhesive systems, higher bond strengths could be achieved with prolonged air-blowing duration between 15-30s. Copyright © 2017 The Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

  3. Effect of Er,Cr:YSGG laser on the microtensile bond strength of two different adhesives to the sound and caries-affected dentin.

    Science.gov (United States)

    Ergücü, Z; Celik, E U; Unlü, N; Türkün, M; Ozer, F

    2009-01-01

    This study examined the effect of Er,Cr:YSGG laser irradiation on the microtensile bond strength (microTBS) of a three-step etch-and-rinse and a two-step self-etch adhesive to sound and caries-affected dentin. Sixteen freshly extracted human molars with occlusal dentin caries were used. The caries lesion was removed by one of the following methods: conventional treatment with burs or Er,Cr:YSGG laser (Waterlase MD, Biolase). The adhesive systems (AdheSE, Ivoclar Vivadent and Scotchbond Multi Purpose, 3M ESPE) were applied to the entire tooth surface according to the manufacturers' instructions. Resin composites were applied to the adhesive-treated dentin surfaces and light-cured. Each tooth was sectioned into multiple beams with the "non-trimming" version of the microtensile test. The specimens were subjected to microtensile forces (BISCO Microtensile Tester, BISCO). The data was analyzed by three-way ANOVA and independent t-tests (p=0.05). Er,Cr:YSGG laser irradiation exhibited similar microTBS values compared to that of conventional bur treatment, regardless of the adhesive system and type of treated dentin. The self-etch system revealed lower microTBS values, both with conventional and laser treatment techniques, compared to the etch-and-rinse adhesive in sound and caries-affected dentin (padhesive systems to sound and caries-affected dentin.

  4. Effect of functional monomers in all-in-one adhesive systems on formation of enamel/dentin acid-base resistant zone.

    Science.gov (United States)

    Nikaido, Toru; Ichikawa, Chiaki; Li, Na; Takagaki, Tomohiro; Sadr, Alireza; Yoshida, Yasuhiro; Suzuki, Kazuomi; Tagami, Junji

    2011-01-01

    This study aimed at evaluating the effect of functional monomers in all-in-one adhesive systems on formation of acid-base resistant zone (ABRZ) in enamel and dentin. Experimental adhesive systems containing one of three functional monomers; MDP, 3D-SR and 4-META were applied to enamel or dentin surface and light-cured. A universal resin composite was then placed. The specimens were subjected to a demineralizing solution (pH 4.5) and 5% NaClO for acid-base challenge and then observed by SEM. The ABRZ was clearly observed in both enamel and dentin interfaces. However, enamel ABRZ was thinner than dentin ABRZ in all adhesives. Morphology of the ABRZ was different between enamel and dentin, and also among the adhesives. Funnel-shaped erosion was observed only in the enamel specimen with the 4-META adhesive. The formation of enamel/dentin ABRZ was confirmed in all adhesives, but the morphology was influenced by the functional monomers.

  5. Effect of saliva contamination on the microshear bond strength of one-step self-etching adhesive systems to dentin.

    Science.gov (United States)

    Yoo, H M; Oh, T S; Pereira, P N R

    2006-01-01

    This study evaluated the effect of saliva contamination and decontamination methods on the dentin bond strength of one-step self-etching adhesive systems. Three commercially available "all-in-one" adhesives (One Up Bond F, Xeno III and Adper Prompt) and one resin composite (Filtek Z-250) were used. Third molars stored in distilled water with 0.5% thymol at 4 degrees C were ground with #600 SiC paper under running water to produce a standardized smear layer. The specimens were randomly divided into groups according to contamination methods: no contamination, which was the control (C); contamination of the adhesive surface with fresh saliva before light curing (A) and contamination of the adhesive surface with fresh saliva after light curing (B). Each contamination group was further subdivided into three subgroups according to the decontamination method: A1-Saliva was removed by a gentle air blast and the adhesive was light-cured; A2-Saliva was rinsed for 10 seconds, gently air-dried and the was adhesive light-cured; A3-Saliva was rinsed and dried as in A2, then the adhesive was re-applied to the dentin surface and light-cured; B1-Saliva was removed with a gentle air blast; B2-Saliva was rinsed and dried; B3-Saliva was rinsed, dried and the adhesive was re-applied and light cured. Tygon tubes filled with resin composite were placed on each surface and light cured. All specimens were stored in distilled water at 37 degrees C for 24 hours. Microshear bond strength was measured using a universal testing machine (EZ test), and data were analyzed by one-way ANOVA followed by the Duncan test to make comparisons among the groups (p0.05). Bond strengths of all B groups were significantly lower compared to the controls (pcontamination after adhesive curing. There was no statistically significant difference among the control groups (p>0.05).

  6. A comparison between new dentinal adhesives (fifth generation and traditional varnish in microleakage reduction of amalgam restorations in primary teeth

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    Mortazavi M. Associate Professor

    2003-06-01

    Full Text Available Statement of Problem: Microleakage presents the major cause for restorations failure in the oral cavity resulting in postoperative sensitivity, pulp irritation and secondary caries formation."nAim: The aim of this study was to compare two dentinal adhesive systems of fifth generation and copalite varnish in reducing microleakage of amalgam restorations in primary teeth."nMaterials and Methods: In this in-vitro study, 100 class V amalgam restorations were prepared on the buccal or lingual surfaces of primary molar and canine teeth. Samples were randomly divided into four groups (25 samples each. No liner was used for the first group and the second group restorations were lined with copalite varnish. Two dentin adhesives, called Syntac C and Single Bond, were used for the third and fourth groups, respectively. At the next stage, the samples were immersed in 5% fuschin solution for 24 hours, then sectioned buccolingually, and examined under a stereomicroscope for microleakage evaluation."nResults: There was a significant difference between four groups statistically (PO.000I, Comparing"nfour groups, the first and fourth ones, demonstrated the most and the least microleakage, respectively."nConclusion: The present study showed that new dentinal adhesive systems caused microleakage"nreduction in amalgam restorations of primary teeth.

  7. [Evaluation of shear bond strengths of self-etching and total-etching dental adhesives to enamel and dentin].

    Science.gov (United States)

    Yu, Ling; Liu, Jing-Ming; Wang, Xiao-Yan; Gao, Xue-Jun

    2009-03-01

    To evaluate the shear bond strengths of four dental adhesives in vitro. The facial surfaces of 20 human maxillary incisors were prepared to expose fresh enamel and randomly divided into four groups, in each group 5 teeth were bonded with one adhesives: group A (Clearfil Protect Bond, self-etching two steps), group B (Adper( Prompt, self-etching one step), group C (SwissTEC SL Bond, total-etching two steps), group D (Single Bond, total-etching two steps). Shear bond strengths were determined using an universal testing machine after being stored in distilled water for 24 h at 37 degrees C. The bond strengths to enamel and dentin were (25.33 +/- 2.84) and (26.07 +/- 5.56) MPa in group A, (17.08 +/- 5.13) and (17.93 +/- 4.70) MPa in group B, (33.14 +/- 6.05) and (41.92 +/- 6.25) MPa in group C, (22.51 +/- 6.25) and (21.45 +/- 7.34) MPa in group D. Group C showed the highest and group B the lowest shear bond strength to enamel and dentin among the four groups. The two-step self-etching adhesive showed comparable shear bond strength to some of the total-etching adhesives and higher shear bond strength than one-step self-etching adhesive.

  8. Effect of Different Protocols in Preconditioning With EDTA in Sclerotic Dentin and Enamel Before Universal Adhesives Applied in Self-etch Mode.

    Science.gov (United States)

    Martini, E C; Parreiras, S O; Gutierrez, M F; Loguercio, A D; Reis, A

    The aim of this study was to investigate the effect of different protocols of 17% ethylene diamine tetra-acetic acid (EDTA) conditioning on the etching pattern and immediate bond strength of universal adhesives to enamel and sclerotic dentin. Forty bovine teeth with sclerotic dentin and 20 human third molars were randomly divided into eight groups resulting from the combination of the main factors surface treatment (none, two-minute EDTA conditioning manual application, 30-second EDTA manual application, 30-second EDTA sonic application) and adhesives systems (Scotchbond Universal Adhesive [SBU] and Prime & Bond Elect [PBE]). Resin-dentin and enamel-dentin bond specimens were prepared and tested under the microtensile bond strength (μTBS) and microshear bond strength (μSBS) tests, respectively. The etching pattern produced on the unground enamel and the sclerotic dentin surfaces under the different protocols and adhesive systems was evaluated under scanning electron microscopy. For enamel, only the main factor adhesive was significant (padhesives in the self-etch mode on sclerotic dentin, mainly when applied for 30 seconds with the aid of a sonic device. EDTA pretreatment also improves the retentive etching pattern of enamel, but it does not result in higher enamel bond strength.

  9. Evaluation of the tensile bond strength of an adhesive system self-etching in dentin irradiated with Er:YAG laser

    International Nuclear Information System (INIS)

    Mello, Andrea Malluf Dabul de

    2000-01-01

    Since Buonocore (1955), several researchers have been seeking for the best adhesive system and treatment for the enamel and dentin surfaces. The use of the acid has been presented as one of the best techniques of dentin conditioning , because this promotes the removal of the 'smear layer and exhibition of dentinal structure, for a best penetration and micro- retention of the adhesive system. However, some conditioning methods have been appearing in the literature, for the substitution or interaction with the acid substances, as the laser. The objective of this work is to evaluate the tensile bond strength of the adhesive system self-etching' associated to a composed resin, in dentin surfaces conditioned with the Er:YAG laser. For this study, freshly extracted human teeth were used and in each one the dentinal surfaces , which were treated with three sandpapers of different granulations (120,400,600), to obtain a standard of the smear layer, before the irradiation of the laser and of the restoring procedure. After these procedures the specimens were storage in distilled water at 37 deg C for 24 hours. Soon after, they were submitted to the tensile strength test .After analyzing the results, we can concluded that the use of the Er:YAG laser can substitute the drill without the need of conditioning, when using the adhesive system 'self-etching' in the dentinal surfaces because there was a decline in the strength of adhesion in the groups conditioned with the laser. (author)

  10. Effect of bur-cut dentin on bond strength using two all-in-one and one two-step adhesive systems.

    Science.gov (United States)

    Koase, Kaori; Inoue, Satoshi; Noda, Mamoru; Tanaka, Toru; Kawamoto, Chiharu; Takahashi, Akiko; Nakaoki, Yasuko; Sano, Hidehiko

    2004-01-01

    To compare the microtensile bond strength (MTBS) of two all-in-one adhesive systems and one experimental two-step self-etching adhesive system to two types of bur-cut dentin. Using one of the three adhesives, Xeno CF Bond (Xeno), Prompt L-Pop (PL), or the experimental two-step system ABF (ABF), resin composite was bonded to flat buccal and root dentin surfaces of eight extracted human premolars. These surfaces were produced using either regular-grit or superfine-grit diamond burs. After storage overnight in 37 degrees C water, the bonded specimens were sectioned into six or seven slices approximately 0.7 mm thick perpendicular to the bonded surface. They were then subjected to microtensile testing. The surfaces of the fractured specimens were observed microscopically to determine the failure mode. In addition, to observe the effect of conditioning, the two types of bur-cut dentin surfaces were conditioned with the adhesives, rinsed with acetone, and observed with SEM. When Xeno and PL were bonded to dentin cut with a regular-grit diamond bur, MTBS values were lower than to superfine bur-cut dentin, and failures occurred adhesively at the interface, whereas the experimental two-step adhesive showed no significant difference in microtensile bond strength between two differently cut surfaces. The all-in-one adhesives tested here improved bond strengths when bonded to superfine bur-cut dentin as a substrate, whereas the experimental two-step adhesive system showed unchanged bonding to both regular and superfine bur-cut dentin surfaces.

  11. New strategy to create “Super Dentin” using adhesive technology: Reinforcement of adhesive–dentin interface and protection of tooth structures

    Directory of Open Access Journals (Sweden)

    Toru Nikaido

    2011-02-01

    Full Text Available Dentin bonding systems have been dramatically simplified and improved during the recent decades. Monomer penetration into dentin and its polymerization in situ creates a hybrid layer, which is essential to obtain good bonding to dentin. Moreover, the presence of an acid–base resistant zone below the hybrid layer has been documented with self-etching adhesive systems in an artificial secondary caries attack. When ultrastructure of the acid–base resistant zone is assessed by SEM and TEM observations, formation of the acid–base resistant zone is considered to be due to the monomer penetration potential and fluoride release in the adhesive systems. Natural dentin has a limited potential to resist an acid attack of secondary caries; however, the acid–base resistant zone does not purely consist of dentin in morphology, it is rather a combination of dentin and the adjacent hybrid layer. Therefore, the reinforced dentin has been called “Super Dentin” bearing the ability to prevent primary and secondary caries. Prospectively, the great potential of adhesive technology in creation of the “Super Dentin” would lead to the development of new materials for mechanical, chemical and biological protection of the dental structures.

  12. Effect of salivary contamination and decontamination on bond strength of two one-step self-etching adhesives to dentin of primary and permanent teeth.

    Science.gov (United States)

    Santschi, Katharina; Peutzfeldt, Anne; Lussi, Adrian; Flury, Simon

    2015-02-01

    To evaluate the effects of human saliva contamination and two decontamination procedures at different stages of the bonding procedure on the bond strength of two one-step self-etching adhesives to primary and permanent dentin. Extracted human primary and permanent molars (210 of each) were ground to mid-coronal dentin. The dentin specimens were randomly divided into 7 groups (n = 15/group/molar type) for each adhesive (Xeno V+ and Scotchbond Universal): no saliva contamination (control); saliva contamination before or after light curing of the adhesives followed by air drying, rinsing with water spray/air drying, or by rinsing with water spray/air drying/reapplication of the adhesives. Resin composite (Filtek Z250) was applied on the treated dentin surfaces. The specimens were stored at 37°C and 100% humidity for 24 h. After storage, shear bond strength (SBS) was measured and data analyzed with nonparametric ANOVA followed by exact Wilcoxon rank sum tests. Xeno V+ generated significantly higher SBS than Scotchbond Universal when no saliva contamination occurred. Saliva contamination reduced SBS of Xeno V+, with the reduction being more pronounced when contamination occurred before light curing than after. In both situations, decontamination involving reapplication of the adhesive restored SBS. Saliva contamination had no significant effect on Scotchbond Universal. There were no differences in SBS between primary and permanent teeth. Rinsing with water and air drying followed by reapplication of the adhesive restored bond strength to saliva-contaminated dentin.

  13. Short communication: pre- and co-curing effect of adhesives on shear bond strengths of composite resins to primary enamel and dentine: an in vitro study.

    Science.gov (United States)

    Viswanathan, R; Shashibhushan, K K; Subba Reddy, V V

    2011-12-01

    To evaluate and compare shear bond strengths of composite resins to primary enamel and dentine when the adhesives are pre-cured (light cured before the application of the resin) or co-cured (adhesive and the resin light cured together). Buccal surfaces of 80 caries-free primary molars were wet ground to create bonding surfaces on enamel and dentine and specimens mounted on acrylic blocks. Two bonding agents (Prime and Bond NT® and Xeno III®) were applied to either enamel or dentine as per manufacturer's instructions. In 40 specimens, the bonding agent was light cured immediately after the application (pre-cured). The other 40 specimens were not light cured until the composite resin application (co-cured). Resin composite cylinders were made incrementally using acrylic moulds over the adhesives and light cured. Specimens were stored in deionised water for 24 hours at room temperature. Shear bond strength was measured using an Instron universal testing machine (in MPa) and was analysed with Student's unpaired t test. Light curing the adhesive separately produced significantly higher bond strengths to primary dentine than co-curing (padhesive separately did not produce significantly different bond strengths to primary enamel (p>0.05). Curing sequence had no significant effect on shear bond strength of adhesives on the primary enamel. Pre-curing adhesives before curing composite resins produced greater shear bond strength to primary dentine.

  14. Effect of double-layer application on dentin bond durability of one-step self-etch adhesives.

    Science.gov (United States)

    Taschner, M; Kümmerling, M; Lohbauer, U; Breschi, L; Petschelt, A; Frankenberger, R

    2014-01-01

    The aim of this in vitro study was 1) to analyze the influence of a double-layer application technique of four one-step self-etch adhesive systems on dentin and 2) to determine its effect on the stability of the adhesive interfaces stored under different conditions. Four different one-step self-etch adhesives were selected for the study (iBondSE, Clearfil S(3) Bond, XenoV(+), and Scotchbond Universal). Adhesives were applied according to manufacturers' instructions or with a double-layer application technique (without light curing of the first layer). After bonding, resin-dentin specimens were sectioned for microtensile bond strength testing in accordance with the nontrimming technique and divided into 3 subgroups of storage: a) 24 hours (immediate bond strength, T0), b) six months (T6) in artificial saliva at 37°C, or c) five hours in 10 % NaOCl at room temperature. After storage, specimens were stressed to failure. Fracture mode was assessed under a light microscope. At T0, iBond SE showed a significant increase in microtensile bond strength when the double-application technique was applied. All adhesive systems showed reduced bond strengths after six months of storage in artificial saliva and after storage in 10% NaOCl for five hours; however at T6, iBond SE, Clearfil S(3) Bond, and XenoV(+) showed significantly higher microtensile bond strength results for the double-application technique compared with the single-application technique. Scotchbond Universal showed no difference between single- or double-application, irrespective of the storage conditions. The results of this study show that improvements in bond strength of one-step self-etch adhesives by using the double-application technique are adhesive dependent.

  15. Effect of repeated use on dentin bond strength of two adhesive systems: All-in-one and one-bottle

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    Shafiei Fershteh

    2009-01-01

    Full Text Available Aims: To compare the effects of repeated use of two one-bottle adhesives with that of two all- in- one adhesives (with acetone solvent on bond strength to dentin. Materials and Methods: A flat dentin surface was prepared on 120 bovine incisors using 600- grit abrasive pape. The teeth were randomly assigned into 12 equal groups. The four adhesive systems [Prime and Bond NT (P&B NT, One-Step Plus (OS, iBond (iB, and G-Bond (GB] were used at baseline, after the lid of the container had been opened 30 times, and after it had been opened 60 times. Before each use of the adhesives, the lids of the containers were left open for 1 min. The resin composites were applied on the dentin in a cylindrical split mold. After thermocycling, shear bond strength test was performed with a universal testing machine at 1 mm/min. We used Kruskal-Wallis and Dunn tests for statistical analysis. Results: There was no statistically significant difference among bond strength (MPa of the groups of P&B NT (31.9 ± 4.6, 31.8 ± 6.5, 26.1 ± 6.7 and OS (33.2 ± 5.1, 30.9 ± 7, 29.3 ± 5.9, respectively (P > 0.05. The mean of the bond strength of iB and GB after 60 times (15.3 ± 4.1 and 12.2 ± 3.9, respectively was significantly lower than that of iB and GB at baseline (23.5 ± 4.8 and 22.2 ± 4.5, respectively (P < 0.05. Conclusions: Repeated use (60 times of the all-in-one adhesive led to a decline in the dentin bond strength. To avoid this problem it would be advisable to have containers with smaller amounts of adhesive or perhaps those with only a singe dose.

  16. Combined effect of smear layer characteristics and hydrostatic pulpal pressure on dentine bond strength of HEMA-free and HEMA-containing adhesives.

    Science.gov (United States)

    Mahdan, Mohd Haidil Akmal; Nakajima, Masatoshi; Foxton, Richard M; Tagami, Junji

    2013-10-01

    This study evaluated the combined effect of smear layer characteristics with hydrostatic pulpal pressure (PP) on bond strength and nanoleakage expression of HEMA-free and -containing self-etch adhesives. Flat dentine surfaces were obtained from extracted human molars. Smear layers were created by grinding with #180- or #600-SiC paper. Three HEMA-free adhesives (Xeno V, G Bond Plus, Beautibond Multi) and two HEMA-containing adhesives (Bond Force, Tri-S Bond) were applied to the dentine surfaces under hydrostatic PP or none. Dentine bond strengths were determined using the microtensile bond test (μTBS). Data were statistically analyzed using three- and two-way ANOVA with Tukey post hoc comparison test. Nanoleakage evaluation was carried out under a scanning electron microscope (SEM). Coarse smear layer preparation and hydrostatic PP negatively affected the μTBS of HEMA-free and -containing adhesives, but there were no significant differences. The combined experimental condition significantly reduced μTBS of the HEMA-free adhesives, while the HEMA-containing adhesives exhibited no significant differences. Two-way ANOVA indicated that for HEMA-free adhesives, there were significant interactions in μTBS between smear layer characteristics and pulpal pressure, while for HEMA-containing adhesives, there were no significant interactions between them. Nanoleakage formation within the adhesive layers of both adhesive systems distinctly increased in the combined experimental group. The combined effect of coarse smear layer preparation with hydrostatic PP significantly reduced the μTBS of HEMA-free adhesives, while in HEMA-containing adhesives, these effects were not obvious. Smear layer characteristics and hydrostatic PP would additively compromise dentine bonding of self-etch adhesives, especially HEMA-free adhesives. Copyright © 2013 Elsevier Ltd. All rights reserved.

  17. Fourier transform infrared photoacoustic spectroscopy study of physicochemical interaction between human dentin and etch-&-rinse adhesives in a simulated moist bond technique

    DEFF Research Database (Denmark)

    Ubaldini, Adriana L M; Baesso, Mauro L; Sehn, Elizandra

    2012-01-01

    systems: (a) 2-hydroxyethylmethacrylate (HEMA) and 4-methacryloxyethyl trimellitate anhydrate (4-META), and (b) HEMA. The Fourier transform infrared photoacoustic spectroscopy was performed before and after dentin treatment with 37% phosphoric acid, with adhesive systems and also for the adhesive systems...

  18. Effect of blood contamination with 1-step self-etching adhesives on microtensile bond strength to dentin.

    Science.gov (United States)

    Yoo, H M; Pereira, P N R

    2006-01-01

    This study evaluated the effect of blood contamination and decontamination methods on the microtensile bond strength of 1-step self-etching adhesive systems to dentin contaminated after adhesive application and light curing. Three commercially available "all-in-one" adhesives (One Up Bond F, Xeno III and Adper Prompt L-Pop) and 1 resin composite (Clearfil AP-X) were used. Third molars that had been stored in distilled water with 0.5% thymol at 4 degrees C were ground with #600 SiC paper under running water to produce a standardized smear layer. The specimens were randomly divided into groups according to the 3 adhesive systems. The adhesive systems were used under 3 conditions: no contamination, which was the control (C); contamination of the light-cured adhesive surface with blood and reapplication of adhesive (Contamination 1) and contamination of the light-cured adhesive surface with blood, then washing, drying and reapplication of the adhesive (Contamination 2). Following light curing of the adhesive, the resin composite was placed in 3 increments up to a 5-mm-thick layer on the bonded surface. All specimens were stored in distilled water at 37 degrees C for 24 hours. The microtensile bond strength was measured using a universal testing machine (EZ test), and data were analyzed by 1-way ANOVA followed by the Duncan test to make comparisons among the groups (p=0.05). After debonding, 5 specimens were selected from each group and examined in a scanning electron microscope to evaluate the modes of fracture. For all adhesives, contamination groups showed lower bond strength than the control (p0.05). For Xeno III and Adper Prompt L-Pop, contamination group #2 showed the lowest bond strength among the groups (pcontamination group #2 showed higher bond strength than contamination group #1 but showed no statistical significance between them (p>0.05).

  19. Influence of laboratory degradation methods and bonding application parameters on microTBS of self-etch adhesives to dentin.

    Science.gov (United States)

    Erhardt, Maria Carolina G; Pisani-Proença, Jatyr; Osorio, Estrella; Aguilera, Fátima S; Toledano, Manuel; Osorio, Raquel

    2011-04-01

    To evaluate the laboratory resistance to degradation and the use of different bonding treatments on resin-dentin bonds formed with three self-etching adhesive systems. Flat, mid-coronal dentin surfaces from extracted human molars were bonded according to manufacturer's directions and submitted to two challenging regimens: (A) chemical degradation with 10% NaOC1 immersion for 5 hours; and (B) fatigue loading at 90 N using 50,000 cycles at 3.0 Hz. Additional dentin surfaces were bonded following four different bonding application protocols: (1) according to manufacturer's directions; (2) acid-etched with 36% phosphoric acid (H3PO4) for 15 seconds; (3) 10% sodium hypochlorite (NaOClaq) treated for 2 minutes, after H3PO4-etching; and (4) doubling the application time of the adhesives. Two one-step self-etch adhesives (an acetone-based: Futurabond/FUT and an ethanol-based: Futurabond NR/FNR) and a two-step self-etch primer system (Clearfil SE Bond/CSE) were examined. Specimens were sectioned into beams and tested for microtensile bond strength (microTBS). Selected debonded specimens were observed under scanning electron microscopy (SEM). Data (MPa) were analyzed by ANOVA and multiple comparisons tests (alpha= 0.05). microTBS significantly decreased after chemical and mechanical challenges (Padhesive systems, regardless the bonding protocol. FUT attained the highest microTBS after doubling the application time. H3PO4 and H3PO4 + NaOCl pretreatments significantly decreased bonding efficacy of the adhesives.

  20. Influence of the number of cycles on shear fatigue strength of resin composite bonded to enamel and dentin using dental adhesives in self-etching mode.

    Science.gov (United States)

    Tsujimoto, Akimasa; Barkmeier, Wayne W; Erickson, Robert L; Takamizawa, Toshiki; Latta, Mark A; Miyazaki, Masashi

    2018-01-30

    The influence of the number of cycles on shear fatigue strength to enamel and dentin using dental adhesives in self-etch mode was investigated. A two-step self-etch adhesive and two universal adhesives were used to bond to enamel and dentin in self-etch mode. Initial shear bond strength and shear fatigue strength to enamel and dentin using the adhesive in self-etch mode were determined. Fatigue testing was used with 20 Hz frequency and cycling periods of 50,000, 100,000 and 1,000,000 cycles, or until failure occurred. For each of the cycling periods, there was no significant difference in shear fatigue strength across the cycling periods for the individual adhesives. Differences in shear fatigue strength were found between the adhesives within the cycling periods. Regardless of the adhesive used in self-etch mode for bonding to enamel or dentin, shear fatigue strength was not influenced by the number of cycles used for shear fatigue strength testing.

  1. Microtensile bond strength of etch-and-rinse and self-etching adhesives to intrapulpal dentin after endodontic irrigation and setting of root canal sealer.

    Science.gov (United States)

    Wattanawongpitak, Nipaporn; Nakajima, Masatoshi; Ikeda, Masaomi; Foxton, Richard M; Tagami, Junji

    2009-02-01

    To evaluate the effect of endodontic irrigation regimens and calcium hydroxide root canal sealer (Sealapex) on the microtensile bond strengths (muTBS) of dual-curing resin composite (Clearfil DC Core Automix) to the intrapulpal dentin. Forty standardized coronal-half root canal dentin specimens obtained from human premolars were divided into 4 groups: group A, no treatment (control); group B, Sealapex; group C, NaOCl/Sealapex; group D, EDTA/NaOCl/Sealapex. After 7 days of storage in 100% relative humidity, Sealapex was removed. Dentin surfaces were bonded with adhesives, either etch-and-rinse (Single Bond) or self-etching (Clearfil SE Bond), and built up with resin composite. The bonded specimens were trimmed into an hourglass shape with a 1-mm2 cross-sectional area for microtensile testing (n = 20). The muTBS to intrapulpal dentin was analyzed using two-way ANOVA and Dunnett's TC test. Two teeth of each group were prepared for micromorphological analysis of dentin surface. The root canal sealer with or without endodontic irrigation significantly affected the bond strengths of resin composite to intrapulpal dentin compared with the control group (p 0.05). The dentin surface was covered with a mud-like material after sealer application for 7 days. The root canal sealer reduced the muTBS of dual-curing resin composite with etch-and-rinse and self-etching adhesive systems to intrapulpal dentin. Treatment with EDTA followed by NaOCI prior to obturation caused an additional reduction in muTBS of both adhesive systems to intrapulpal dentin.

  2. Effect of smear layer deproteinization on bonding of self-etch adhesives to dentin: a systematic review and meta-analysis

    Science.gov (United States)

    Alshaikh, Khaldoan H.; Mahmoud, Salah H.

    2018-01-01

    Objectives The aim of this systematic review was to critically analyze previously published studies of the effects of dentin surface pretreatment with deproteinizing agents on the bonding of self-etch (SE) adhesives to dentin. Additionally, a meta-analysis was conducted to quantify the effects of the above-mentioned surface pretreatment methods on the bonding of SE adhesives to dentin. Materials and Methods An electronic search was performed using the following databases: Scopus, PubMed and ScienceDirect. The online search was performed using the following keywords: ‘dentin’ or ‘hypochlorous acid’ or ‘sodium hypochlorite’ and ‘self-etch adhesive.’ The following categories were excluded during the assessment process: non-English articles, randomized clinical trials, case reports, animal studies, and review articles. The reviewed studies were subjected to meta-analysis to quantify the effect of the application time and concentration of sodium hypochlorite (NaOCl) and hypochlorous acid (HOCl) deproteinizing agents on bonding to dentin. Results Only 9 laboratory studies fit the inclusion criteria of this systematic review. The results of the meta-analysis revealed that the pooled average microtensile bond strength values to dentin pre-treated with deproteinizing agents (15.71 MPa) was significantly lower than those of the non-treated control group (20.94 MPa). Conclusions In light of the currently available scientific evidence, dentin surface pretreatment with deproteinizing agents does not enhance the bonding of SE adhesives to dentin. The HOCl deproteinizing agent exhibited minimal adverse effects on bonding to dentin in comparison with NaOCl solutions. PMID:29765895

  3. Efficacy of Hydrophobic Layer On Sealing Ability of Dentin Adhesive Systems in Class V Composite Resin Restorations

    Directory of Open Access Journals (Sweden)

    Fatemeh Maleknejad

    2011-03-01

    Full Text Available Background and aims. Adhesive permeability is hindered by application of an additional layer of hydrophobic resin, which increases its concentration within the hydrophilic layer, reduces its affinity to water, and enhances its physical properties. The aim of the present study was to evaluate the effect of a hydrophobic layer on the microleakage of class V composite restorations using different adhesives. Materials and methods. The adhesives including total-etch Scotchbond MP and Single Bond, and the self-etch Clearfil SE Bond and Clearfil S3 Bond were applied to 80 class V cavities in vitro on the buccal surface in CEJ and then were followed by hydrophobic resin (Margin Bond in half of the cavities in each group (n=10. After restoration with microhybrid composite, Z100 and immersion in fuchsine, the degree of microleakage was assessed. Data were analyzed using the Kruskal-Wallis, Man-Whitney, and Wilcoxon tests. Results. The hydrophobic layer significantly reduced the microleakage of Clearfil SE Bond and Clearfil S3 Bond only in dentin (p0.05. Conclusion. Within the limitation of this study, only Clearfil S3 Bond could demonstrate the identical values of microleakage in enamel and dentinal margins.

  4. Correlation between degree of conversion, resin-dentin bond strength and nanoleakage of simplified etch-and-rinse adhesives.

    Science.gov (United States)

    Hass, Viviane; Dobrovolski, Max; Zander-Grande, Christiana; Martins, Gislaine Cristine; Gordillo, Luís Alfonso Arana; Rodrigues Accorinte, Maria de Lourdes; Gomes, Osnara Maria Mongruel; Loguercio, Alessandro Dourado; Reis, Alessandra

    2013-09-01

    The aim of this study was to correlate the degree of conversion measured inside the hybrid layer (DC) with the microtensile resin-dentin bond strength (μTBS) and silver nitrate uptake or nanoleakage (SNU) for five simplified etch-and-rinse adhesive systems. Fifty-five caries free extracted molars were used in this study. Thirty teeth were used for μTBS/SNU [n=6] and 25 teeth for DC [n=5]. The dentin surfaces were bonded with the following adhesives: Adper Single Bond 2 (SB), Ambar (AB), XP Bond (XP), Tetric N-Bond (TE) and Stae (ST) followed by composite resin build-ups. For μTBS and SNU test, bonded teeth were sectioned in order to obtain stick-shaped specimens (0.8mm(2)), which were tested under tensile stress (0.5mm/min). Three bonded sticks, from each tooth, were not tested in tensile stress and they were immersed in 50% silver nitrate, photo-developed and analyzed by scanning electron microscopy. Longitudinal 1-mm thick sections were prepared for the teeth assigned for DC measurement and evaluated by micro-Raman spectroscopy. ST showed lowest DC, μTBS, and higher SNU (p0.05), except for TE which showed an intermediate SNU level. The DC was positively correlated with μTBS and negatively correlated with SNU (p<0.05). SNU was also negatively correlated with μTBS (p<0.05). The measurement of DC inside the hybrid layer can provide some information about bonding performance of adhesive systems since this property showed a good correlation with resin-dentin bond strength and SNU values. Copyright © 2013 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

  5. Effect of ferric sulfate contamination on the bonding effectiveness of etch-and-rinse and self-etch adhesives to superficial dentin

    OpenAIRE

    Shahram Farzin Ebrahimi; Niloofar Shadman; Arezoo Abrishami

    2013-01-01

    Aim: This study investigated the effect of one hemostatic agent on the shear bond strength of self-etch and etch-and-rinse adhesive systems. Materials and Methods: Sixty extracted third molars were selected. After preparing a flat surface of superficial dentin, they were randomly divided into six groups. Adhesives were Tetric N-Bond, AdheSE, and AdheSE One F. Before applying adhesives, surfaces were contaminated with ViscoStat for 60 s in three groups and rinsed. Then composite were attached ...

  6. Effects of solvent volatilization time on the bond strength of etch-and-rinse adhesive to dentin using conventional or deproteinization bonding techniques.

    Science.gov (United States)

    de Sousa Júnior, José Aginaldo; Carregosa Santana, Márcia Luciana; de Figueiredo, Fabricio Eneas Diniz; Faria-E-Silva, André Luis

    2015-08-01

    This study determined the effect of the air-stream application time and the bonding technique on the dentin bond strength of adhesives with different solvents. Furthermore, the content and volatilization rate of the solvents contained in the adhesives were also evaluated. Three adhesive systems with different solvents (Stae, SDI, acetone; XP Bond, Dentsply De Trey, butanol; Ambar, FGM, ethanol) were evaluated. The concentrations and evaporation rates of each adhesive were measured using an analytical balance. After acid-etching and rinsing, medium occlusal dentin surfaces of human molars were kept moist (conventional) or were treated with 10% sodium hypochlorite for deproteinization. After applying adhesives over the dentin, slight air-stream was applied for 10, 30 or 60 sec. Composite cylinders were built up and submitted to shear testing. The data were submitted to ANOVA and Tukey's test (α = 0.05). Stae showed the highest solvent content and Ambar the lowest. Acetone presented the highest evaporation rate, followed by butanol. Shear bond strengths were significantly affected only by the factors of 'adhesive' and 'bonding technique' (p Stae showed the lowest bond strength values (p < 0.05), while no significant difference was observed between XP Bond and Ambar. Despite the differences in content and evaporation rate of the solvents, the duration of air-stream application did not affect the bond strength to dentin irrespective of the bonding technique.

  7. Long-term dentin retention of etch-and-rinse and self-etch adhesives and a resin-modified glass ionomer cement in non-carious cervical lesions

    DEFF Research Database (Denmark)

    Dijken, J.W.V. van; Pallesen, U.

    2008-01-01

    The aim of this study was to evaluate the clinical long-term retention to dentin of seven adhesive systems.......The aim of this study was to evaluate the clinical long-term retention to dentin of seven adhesive systems....

  8. In vitro evaluation of influence of salivary contamination on the dentin bond strength of one-bottle adhesive systems

    Directory of Open Access Journals (Sweden)

    Nujella B.P Suryakumari

    2011-01-01

    Full Text Available Aim: To evaluate the effect of salivary contamination on the bond strength of one-bottle adhesive systems - (the V generation at various stages during the bonding procedure and to investigate the effect of the contaminant removing treatments on the recovery of bond strengths. Materials and Methods: In this study the V generation one-bottle system - (Adper Single Bond was tested. Fifty caries-free human molars with flat dentin surfaces were randomly divided into five groups of ten teeth each: Group I had 15 second etching with 35% Ortho Phosphoric acid, 15 second rinse and blot dried (Uncontaminated; Group II contaminated and blot dried; Group III contaminated and completely dried; Group IV contaminated, washed, blot dried; Group V contaminated, retched washed, and blot dried. The bonding agent was applied and resin composite (Z-100 3M ESPE was bonded to the treated surfaces using the Teflon mold. The specimens in each group were then subjected to shear bond strength testing in an Instron Universal testing machine at a crosshead speed of 1 mm / minute and the data were subjected to one way ANOVA for comparison among the groups (P<0.05. Results: There was a significant difference between the group that was dried with strong oil-free air after contamination (Group III and the other groups. When the etched surface was contaminated by saliva, there was no statistical difference between the just blot dry, wash, or the re-etching groups (Groups II, IV, V if the dentin surface was kept wet before priming. When the etched dentin surface was dried (Group III the shear bond strength decreased considerably. Conclusion: The bond strengths to the tooth structure of the recent dentin bonding agents are less sensitive to common forms of contamination than assumed. Re-etching without additional mechanical preparation is sufficient to provide or achieve the expected bond strength.

  9. Influence of a hydrophobic resin coating on the immediate and 6-month dentin bonding of three universal adhesives.

    Science.gov (United States)

    Sezinando, Ana; Luque-Martinez, Issis; Muñoz, Miguel Angel; Reis, Alessandra; Loguercio, Alessandro D; Perdigão, Jorge

    2015-10-01

    To test the influence of a hydrophobic resin coating (HC) on the immediate (24h) and 6-month (6m) microtensile dentin bond strengths (μTBS) and nanoleakage (NL) of three universal adhesives applied in self-etch (SE) or in etch-and-rinse (ER) mode. Sixty caries-free extracted third molars were assigned to 12 experimental groups resulting from the combination of the factors "adhesive system" (Scotchbond Universal Adhesive [SBU], 3M ESPE; All-Bond Universal [ABU], Bisco Inc.; and G-Bond Plus [GBP], GC Corporation); "adhesive strategy" (SE or ER); "hydrophobic resin coating" [HC] (with or without Heliobond, Ivoclar Vivadent); and "storage time" (24h or 6m). Specimens were prepared for μTBS testing - (24h) half of the beams were immediately tested under tension; and (6m) the other half was stored in distilled water (37°C) for 6m prior to testing. For each tooth, two beams were randomly selected for NL evaluation for both evaluation times. Data were analyzed for each adhesive system using three-way ANOVA and Tukey's post-hoc test (α=0.05). μTBS: (24h): In SE mode, HC resulted in statistically greater mean μTBS for all adhesives. (6m): When HC was not used the mean μTBS for SBU/ER, ABU/ER, GBP/ER and SBU/SE decreased significantly. NL: (24h): SBU/ER, ABU/ER and GBP/SE resulted in a significant reduction in NL when HC was applied. (6m): No significant reduction was observed for SBU/ER or for SBU/SE regardless of the use of HC. The application of a hydrophobic resin coating improved the 24h and the 6m performances of all three adhesives systems in SE mode. Copyright © 2015 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

  10. Effects of solvent volatilization time on the bond strength of etch-and-rinse adhesive to dentin using conventional or deproteinization bonding techniques

    Directory of Open Access Journals (Sweden)

    José Aginaldo de Sousa Júnior

    2015-08-01

    Full Text Available Objectives This study determined the effect of the air-stream application time and the bonding technique on the dentin bond strength of adhesives with different solvents. Furthermore, the content and volatilization rate of the solvents contained in the adhesives were also evaluated. Materials and Methods Three adhesive systems with different solvents (Stae, SDI, acetone; XP Bond, Dentsply De Trey, butanol; Ambar, FGM, ethanol were evaluated. The concentrations and evaporation rates of each adhesive were measured using an analytical balance. After acid-etching and rinsing, medium occlusal dentin surfaces of human molars were kept moist (conventional or were treated with 10% sodium hypochlorite for deproteinization. After applying adhesives over the dentin, slight air-stream was applied for 10, 30 or 60 sec. Composite cylinders were built up and submitted to shear testing. The data were submitted to ANOVA and Tukey's test (α = 0.05. Results Stae showed the highest solvent content and Ambar the lowest. Acetone presented the highest evaporation rate, followed by butanol. Shear bond strengths were significantly affected only by the factors of 'adhesive' and 'bonding technique' (p < 0.05, while the factor 'duration of air-stream' was not significant. Deproteinization of dentin increased the bond strength (p < 0.05. Stae showed the lowest bond strength values (p < 0.05, while no significant difference was observed between XP Bond and Ambar. Conclusions Despite the differences in content and evaporation rate of the solvents, the duration of air-stream application did not affect the bond strength to dentin irrespective of the bonding technique.

  11. Initial and long-term bond strengths of one-step self-etch adhesives with silane coupling agent to enamel-dentin-composite in combined situation.

    Science.gov (United States)

    Mamanee, Teerapong; Takahashi, Masahiro; Nakajima, Masatoshi; Foxton, Richard M; Tagami, Junji

    2015-01-01

    This study evaluated the effect of adding silane coupling agent on initial and long-term bond strengths of one-step self-etch adhesives to enamel-dentin-composite in combined situation. Cervical cavities were prepared on extracted molars and filled with Clearfil AP-X. After water-storage for one-week, the filled teeth were sectioned in halves to expose enamel, dentin and composite surfaces and then enamel-dentin-composite surface was totally applied with one of adhesive treatments (Clearfil SE One, Clearfil SE One with Clearfil Porcelain Bond Activator, Beautibond Multi, Beautibond Multi with Beautibond Multi PR Plus and Scotchbond Universal). After designed period, micro-shear bond strengths (µSBSs) to each substrate were determined. For each period of water-storage, additive silane treatments significantly increased µSBS to composite (penamel (p>0.05). Moreover, the stability of µSBS was depended on materials and substrates used.

  12. Comparison of shear bond strength of self-etch and self-adhesive cements bonded to lithium disilicate, enamel and dentin.

    Science.gov (United States)

    Naranjo, Jennifer; Ali, Mohsin; Belles, Donald

    2015-11-01

    Comparison of shear bond strength of self-etch and self-adhesive cements bonded to lithium disilicate, enamel and dentin. With several self-adhesive resin cements currently available, there is confusion about which product and technique is optimal for bonding ceramic restorations to teeth. The objective of this study was to compare the shear bond strength of lithium disilicate cemented to enamel and dentin using 5 adhesive cements. 100 lithium disilicate rods were pretreated with 5% hydrofluoric acid, silane, and cemented to 50 enamel and 50 dentin surfaces using five test cements: Variolink II (etch-and-rinse) control group, Clearfil Esthetic (two step self-etch), RelyX Unicem, SpeedCEM, and BifixSE (self-adhesive). All specimens were stored (37 degrees C, 100% humidity) for 24 hours before testing their shear bond strength using a universal testing machine (Instron). Debonded surfaces were observed under a low-power microscope to assess the location and type of failure. The highest bond strength for both enamel and dentin were recorded for Variolink II, 15.1MPa and 20.4MPa respectively, and the lowest were recorded for BifixSE, 0.6MPa and 0.9MPa respectively. Generally, higher bond strengths were found for dentin (7.4MPa) than enamel (5.3MPa). Tukey's post hoc test showed no significant difference between Clearfil Esthetic and SpeedCem (p = 0.059), Unicem and SpeedCem (p = 0.88), and Unicem and BifixSE (p = 0.092). All cements bonded better to lithium disilicate than to enamel or dentin, as all bond failures occurred at the tooth/adhesive interface except for Variolink II. Bond strengths recorded for self-adhesive cements were very low compared to the control "etch and rinse" and self-etch systems. Further improvements are apparently needed in self-adhesive cements for them to replace multistep adhesive systems. The use of conventional etch and rinse cements such as Veriolink II should be preferred for cementing all ceramic restorations over self-adhesive cements

  13. In vivo model for microbial invasion of tooth root dentinal tubules

    Directory of Open Access Journals (Sweden)

    Jane L. BRITTAN

    2016-04-01

    Full Text Available ABSTRACT Objective Bacterial penetration of dentinal tubules via exposed dentine can lead to root caries and promote infections of the pulp and root canal system. The aim of this work was to develop a new experimental model for studying bacterial invasion of dentinal tubules within the human oral cavity. Material and Methods Sections of human root dentine were mounted into lower oral appliances that were worn by four human subjects for 15 d. Roots were then fixed, sectioned, stained and examined microscopically for evidence of bacterial invasion. Levels of invasion were expressed as Tubule Invasion Factor (TIF. DNA was extracted from root samples, subjected to polymerase chain reaction amplification of 16S rRNA genes, and invading bacteria were identified by comparison of sequences with GenBank database. Results All root dentine samples with patent tubules showed evidence of bacterial cell invasion (TIF value range from 5.7 to 9.0 to depths of 200 mm or more. A spectrum of Gram-positive and Gram-negative cell morphotypes were visualized, and molecular typing identified species of Granulicatella, Streptococcus, Klebsiella, Enterobacter, Acinetobacter, and Pseudomonas as dentinal tubule residents. Conclusion A novel in vivo model is described, which provides for human root dentine to be efficiently infected by oral microorganisms. A range of bacteria were able to initially invade dentinal tubules within exposed dentine. The model will be useful for testing the effectiveness of antiseptics, irrigants, and potential tubule occluding agents in preventing bacterial invasion of dentine.

  14. Laboratory evaluation of the effect of unfilled resin after the use of self-etch and total-etch dentin adhesives on the Shear Bond Strength of composite to dentin.

    Science.gov (United States)

    Nasseri, Ehsan Baradaran; Majidinia, Sara; Sharbaf, Davood Aghasizadeh

    2017-05-01

    Based on the frequent application of composite resins as tooth-colored fillings, this method is considered a viable alternative to dental amalgam. However, this method has the low bond strength of the composite to dentin. To solve this issue, various dental adhesive systems with different characteristics have been developed by dentistry experts. To assess the effect of an additional layer of unfilled resin in self-etch and total-etch dentin adhesives on the shear bond strength (SBS) of composite to dentin. Moreover, we assessed the effects of sample storage in artificial saliva on the SBS of composite to dentin. Methods: This experimental study was conducted on 160 freshly extracted human first or second premolar teeth, which were randomly divided into 16 groups. The teeth were prepared from Mashhad University of Medical Sciences, Mashhad, Iran (2008-2009). Scotchbond Multi-purpose (SBMP), single bond (SB), Clearfil SE Bond, and Clearfil S3 Bond were applied to dentin surface with or without the placement of hydrophobic resin (Margin Bond) in accordance with the instructions of the manufacturers. To expose the coronal dentin, the teeth were abraded with 600 grit SiC paper. Immediately after restoration, half of the samples were tested in terms of SBS, while the other samples were evaluated in terms of SBS after three months of storage in artificial saliva. SBS rates of dental composites evaluated by universal testing machine and samples were studied by optical stereomicroscopy to verify the failure type. Data analysis was performed in SPSS V.16 using Kolmogorov-Smirnov test, independent-samples t-test, ANOVA, and Duncan's logistic regression test. In this study, a significant reduction was observed in the SBS rates of SB and S3 bond adhesive systems after storage with and without hydrophobic resin (p>0.000). Without storage in normal saline, a significant increase was observed in the SBS rate of the SE bond (p=0.013). In addition, SBS rate of SBMP significantly

  15. Influence of Air Abrasion and Sonic Technique on Microtensile Bond Strength of One-Step Self-Etch Adhesive on Human Dentin

    Directory of Open Access Journals (Sweden)

    Baraba Anja

    2015-01-01

    Full Text Available The purpose of this in vitro study was to evaluate the microtensile bond strength of one-step self-etch adhesive to human dentin surface modified with air abrasion and sonic technique and to assess the morphological characteristics of the pretreated dentin surface. The occlusal enamel was removed to obtain a flat dentin surface for thirty-six human molar teeth. The teeth were randomly divided into three experimental groups (n = 12 per group, according to the pretreatment of the dentin: (1 control group, (2 air abrasion group, and (3 sonic preparation group. Microtensile bond strength test was performed on a universal testing machine. Two specimens from each experimental group were subjected to SEM examination. There was no statistically significant difference in bond strength between the three experimental groups (P > 0.05. Mean microtensile bond strength (MPa values were 35.3 ± 12.8 for control group, 35.8 ± 13.5 for air abrasion group, and 37.7 ± 12.0 for sonic preparation group. The use of air abrasion and sonic preparation with one-step self-etch adhesive does not appear to enhance or impair microtensile bond strength in dentin.

  16. Evaluating the shear bond strength of enamel and dentin with or without etching: A comparative study between dimethacrylate-based and silorane-based adhesives

    Science.gov (United States)

    Hajizadeh, Hila; Nasseh, Atefeh; Rahmanpour, Naim

    2015-01-01

    Background Silorane-based composites and their specific self-etch adhesive were introduced to conquest the polymerization shrinkage of methacrylate-based composites. It has been shown that additional etching of enamel and dentin can improve the bond strength of self-etch methacrylate-based adhesives but this claim is not apparent about silorane-based adhesives. Our objective was to compare the shear bond strength (SBS) of enamel and dentin between silorane-based adhesive resin and a methacrylate-based resin with or without additional etching. Material and Methods 40 sound human premolars were prepared and divided into two groups: 1- Filtek P60 composite and Clearfil SE Bond adhesive; 2- Filtek P90 composite and Silorane adhesive. Each group divided into two subgroups: with or without additional etching. For additional etching, 37% acid phosphoric was applied before bonding procedure. A cylinder of the composite was bonded to the surface. After 24 hours storage and 500 thermo cycling between 5-55°C, shear bond strength was assessed with the cross head speed of 0.5 mm/min. Then, bonded surfaces were observed under stereomicroscope to determine the failure mode. Data were analyzed with two-way ANOVA and Fischer exact test. Results Shear bond strength of Filtek P60 composite was significantly higher than Filtek P90 composite both in enamel and dentin surfaces (Penamel or dentin for each of the composites (P>0.05). There was no interaction between composite type and additional etching (P>0.05). Failure pattern was mainly adhesive and no significant correlation was found between failure and composite type or additional etching (P>0.05). Conclusions Shear bond strength of methacrylate-based composite was significantly higher than silorane-based composite both in enamel and dentin surfaces and additional etching had no significant effect on shear bond strength in enamel or dentin for each of the composites. The mode of failure had no meaningful relation to the type of

  17. Comparison of the shear bond strength of self-adhesive resin cements to enamel and dentin with different protocol of application.

    Science.gov (United States)

    Moghaddas, Mohammad Javad; Hossainipour, Zahra; Majidinia, Sara; Ojrati, Najmeh

    2017-08-01

    The aim of the present study was to determine the shear bond strength of self-adhesive resin cements to enamel and dentin with and without surface treatments, and compare them with conventional resin cement as the control group. In this experimental study, buccal and lingual surface of the thirty sound human premolars were polished in order to obtain a flat surface of enamel (E) in buccal, and dentin (D) in lingual. Sixty feldspathic ceramic blocks (2×3×3 mm) were prepared and randomly divided into six groups (n=10). Each block was cemented to the prepared surface (30 enamel and 30 dentin surface) according to different protocol: E1 and D1; RelyX ARC as control group, E2, D2; RelyX Unicem, E3, D3; acid etching +RelyX Unicem. The specimens were termocycled and subjected to shear forces by a universal testing machine at a cross head speed of 0.5 mm/min. The mode of fracture were evaluated by stereomicroscope. Data were analyzed with descriptive statistical methods using SPSS version 15. One-way ANOVA, and post hoc Tukey tests were used to compare bond strengths between the groups with different adhesives at α=0.05. Statistical analysis showed no significant differences within the enamel subgroups, but there were significant differences within the dentinal subgroups, and statistically significant differences were found between the groups D1and D3 (p=0.02). Comparison between similar enamel and dentinal subgroups showed that there was a significant difference just between the subgroups E3 and D3 (p=0.01). Elective etching of enamel did not lead to significant increase in the shear bond strength of RelyX Unicem in comparison to RelyX ARC. On the other hand, elective etching of dentin reduces the bond strength of RelyX Unicem with the dentin.

  18. The role of copper nanoparticles in an etch-and-rinse adhesive on antimicrobial activity, mechanical properties and the durability of resin-dentine interfaces.

    Science.gov (United States)

    Gutiérrez, Mario F; Malaquias, Pamela; Hass, Viviane; Matos, Thalita P; Lourenço, Lucas; Reis, Alessandra; Loguercio, Alessandro D; Farago, Paulo Vitor

    2017-06-01

    To evaluate the effect of addition of copper nanoparticles at different concentrations into an etch-and-rinse adhesive (ER) on antimicrobial activity, Knoop microhardness (KHN), in vitro and in situ degree of conversion (DC), as well as the immediate (IM) and 2-year (2Y) resin-dentine bond strength (μTBS) and nanoleakage (NL). Seven experimental ER adhesives were formulated according to the amount of copper nanoparticles incorporated into the adhesives (0 [control], 0.0075 to 1wt.%). We tested the antimicrobial activity of adhesives against Streptococcus mutans using agar diffusion assay after IM and 2Y. The Knoop microhardness and in vitro DC were tested after IM and 2Y. The adhesives were applied to flat occlusal dentine surfaces after acid etching. After resin build-ups, specimens were longitudinally sectioned to obtain beam-like resin-dentine specimens (0.8mm 2 ), which were used for evaluation of μTBS and nanoleakage at the IM and 2Y periods. In situ DC was evaluated at the IM period in these beam-like specimens. Data were submitted to appropriate statistical analyses (α=0.05). The addition of copper nanoparticles provided antimicrobial activity to the adhesives only in the IM evaluation and slightly reduced the KHN, the in vitro and in situ DC (copper concentrations of 1wt.%). However, KHN increase for all concentrations after 2Y. After 2Y, no significant reductions of μTBS (0.06 to 1% wt.%) and increases of nanoleakage were observed for copper containing adhesives compared to the control group. Copper nanoparticles addition up to 0.5wt.% may provide antimicrobial properties to ER adhesives and prevent the degradation of the adhesive interface, without reducing the mechanical properties of the formulations. Copyright © 2017 Elsevier Ltd. All rights reserved.

  19. Multivariate analysis of attenuated total reflection Fourier transform infrared (ATR FT-IR) spectroscopic data to confirm phase partitioning in methacrylate-based dentin adhesive.

    Science.gov (United States)

    Ye, Qiang; Parthasarathy, Ranganathan; Abedin, Farhana; Laurence, Jennifer S; Misra, Anil; Spencer, Paulette

    2013-12-01

    Water is ubiquitous in the mouths of healthy individuals and is a major interfering factor in the development of a durable seal between the tooth and composite restoration. Water leads to the formation of a variety of defects in dentin adhesives; these defects undermine the tooth-composite bond. Our group recently analyzed phase partitioning of dentin adhesives using high-performance liquid chromatography (HPLC). The concentration measurements provided by HPLC offered a more thorough representation of current adhesive performance and elucidated directions to be taken for further improvement. The sample preparation and instrument analysis using HPLC are, however, time-consuming and labor-intensive. The objective of this work was to develop a methodology for rapid, reliable, and accurate quantitative analysis of near-equilibrium phase partitioning in adhesives exposed to conditions simulating the wet oral environment. Analysis by Fourier transform infrared (FT-IR) spectroscopy in combination with multivariate statistical methods, including partial least squares (PLS) regression and principal component regression (PCR), were used for multivariate calibration to quantify the compositions in separated phases. Excellent predictions were achieved when either the hydrophobic-rich phase or the hydrophilic-rich phase mixtures were analyzed. These results indicate that FT-IR spectroscopy has excellent potential as a rapid method of detection and quantification of dentin adhesives that experience phase separation under conditions that simulate the wet oral environment.

  20. Six-month evaluation of adhesives interface created by a hydrophobic adhesive to acid-etched ethanol-wet bonded dentine with simplified dehydration protocols.

    Science.gov (United States)

    Sadek, Fernanda T; Mazzoni, Annalisa; Breschi, Lorenzo; Tay, Franklin R; Braga, Roberto R

    2010-04-01

    To evaluate the efficacy of simplified dehydration protocols, in the absence of tubular occlusion, on bond strength and interfacial nanoleakage of a hydrophobic experimental adhesive blend to acid-etched, ethanol-dehydrated dentine immediately and after 6 months. Molars were randomly assigned to 6 treatment groups (n=5). Under pulpal pressure simulation, dentine crowns were acid-etched with 35% H(3)PO(4) and rinsed with water. Adper Scotchbond Multi-Purpose was used for the control group. The remaining groups had their dentine surface dehydrated with ethanol solutions: group 1=50%, 70%, 80%, 95% and 3x100%, 30s for each application; group 2 the same ethanol sequence with 15s for each solution; groups 3, 4 and 5 used 100% ethanol only, applied in seven, three or one 30s step, respectively. After dehydration, a primer (50% BisGMA+TEGDMA, 50% ethanol) was used, followed by the neat comonomer adhesive application. Resin composite build-ups were then prepared using an incremental technique. Specimens were stored for 24h, sectioned into beams and stressed to failure after 24h or after 6 months of artificial ageing. Interfacial silver leakage evaluation was performed for both storage periods (n=5 per subgroup). Group 1 showed higher bond strengths at 24h or after 6 months of ageing (45.6+/-5.9(a)/43.1+/-3.2(a)MPa) and lower silver impregnation. Bond strength results were statistically similar to control group (41.2+/-3.3(ab)/38.3+/-4.0(ab)MPa), group 2 (40.0+/-3.1(ab)/38.6+/-3.2(ab)MPa), and group 3 at 24h (35.5+/-4.3(ab)MPa). Groups 4 (34.6+/-5.7(bc)/25.9+/-4.1(c)MPa) and 5 (24.7+/-4.9(c)/18.2+/-4.2(c)MPa) resulted in lower bond strengths, extensive interfacial nanoleakage and more prominent reductions (up to 25%) in bond strengths after 6 months of ageing. Simplified dehydration protocols using one or three 100% ethanol applications should be avoided for the ethanol-wet bonding technique in the absence of tubular occlusion, as they showed decreased bond strength, more

  1. Influence of chlorhexidine and/or ethanol treatment on bond strength of an etch-and-rinse adhesive to dentin: an in vitro and in situ study.

    Science.gov (United States)

    Simões, D M S; Basting, R T; Amaral, F L B; Turssi, C P; França, F M G

    2014-01-01

    The aim of this study was to evaluate the effect of a chlorhexidine and/or ethanol application on the bond strength of an etch-and-rinse, hydrophobic adhesive system either under in vitro aging or in situ cariogenic challenge. The dentin surface of 36 human third molars were flattened and allocated into four groups to be treated with chlorhexidine, ethanol, or chlorhexidine + ethanol or left unexposed to any solution (control) (n=9). Then, a resin composite restoration was made on the dentin surface and longitudinal sticks were obtained. Sticks from each tooth were assigned to three test conditions: stored in water in vitro for 24 hours, stored in water in vitro for 6 months, or worn in situ for 14 days. During in situ wear time, a high-cariogenic challenge condition was simulated. Specimens were tested for microtensile bond strength (μTBS). Multivariate analysis of variance and Tukey's test showed that chlorhexidine, ethanol, or chlorhexidine + ethanol did not affect the μTBS. The in vitro μTBS values were significantly lower for the specimens stored for 6 months than for those stored for 24 hours. Intermediate μTBS values were shown by the specimens worn in situ. Thus, use of chlorhexidine and/or ethanol was incapable of containing the degradation at the bond interface in the in vitro model. The in situ model was capable of reducing bond strength similarly to the in vitro/6 months model. Despite this, the in situ bond strength was still similar to that of the in vitro/24-hour model.

  2. Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin

    Directory of Open Access Journals (Sweden)

    Carolina CARVALHO

    Full Text Available ABSTRACT Objective Green tea extract has been advocated as a matrix metalloproteinase (MMP inhibitor; however, its effect on bond durability to caries-affected dentin has never been reported. Thus, the aim of this in vitro study was to evaluate the effect of two MMP inhibitors (2% chlorhexidine and 2% green tea extract, applied after acid etching, on bond durability of an etch-and-rinse adhesive system to caries-affected dentin. Material and Methods Occlusal enamel was removed from third molars to expose the dentin surface, and the molars were submitted to a caries induction protocol for 15 days. After removal of infected dentin, specimens were conditioned with 37% phosphoric acid (15 seconds and randomly divided into three groups, according to the type of dentin pretreatment (n=10: NT: no treatment; GT: 2% green tea extract; CLX: 2% chlorhexidine. The etch-and-rinse adhesive system (Adper™ Single Bond 2, 3M ESPE, St. Paul, MN, USA was applied according to the manufacturer's instructions, and composite resin restorations were built on the dentin. After 24 hours, at 37°C, the resin-tooth blocks were sectioned perpendicularly to the adhesive interface in the form of sticks (0.8 mm2 of adhesive area and randomly subdivided into two groups according to when they were to be submitted to microtensile bond strength (μTBS testing: immediately or 6 months after storage in distilled water. Data were reported in MPa and submitted to two-way ANOVA for completely randomized blocks, followed by Tukey’s test (α=0.05. Results After 24 hours, there was no significant difference in the μTBS of the groups. After 6 months, the GT group had significantly higher μTBS values. Conclusion It was concluded that the application of 2% green tea extract was able to increase bond durability of the etch-and-rinse system to dentin. Neither the application of chlorhexidine nor non-treatment (NT - control had any effect on bond strength after water storage.

  3. Adaptation of adhesive post and cores to dentin after in vitro occlusal loading: evaluation of post material influence.

    Science.gov (United States)

    Dietschi, Dider; Ardu, Stefano; Rossier-Gerber, Anne; Krejci, Ivo

    2006-12-01

    Fatigue resistance of post and cores is critical to the long term behavior of restored nonvital teeth. The purpose of this in vitro trial was to evaluate the influence of the post material's physical properties on the adaptation of adhesive post and core restorations after cyclic mechanical loading. Composite post and cores were made on endodontically treated deciduous bovine teeth using 3 anisotropic posts (made of carbon, quartz, or quartz-and-carbon fibers) and 3 isotropic posts (zirconium, stainless steel, titanium). Specimens were submitted to 3 successive loading phases--250,000 cycles at 50 N, 250,000 at 75 N, and 500,000 at 100 N--at a rate of 1.5 Hz. Restoration adaptation was evaluated under SEM, before and during loading (margins) and after test completion (margins and internal interfaces). Six additional samples were fabricated for the characterization of interface micromorphology using confocal microscopy. Mechanical loading increased the proportion of marginal gaps in all groups; carbon fiber posts presented the lowest final gap proportion (7.11%) compared to other stiffer metal-ceramic or softer fiber posts (11.0% to 19.1%). For internal adaptation, proportions of debonding between dentin and core or cement varied from 21.69% (carbon post) to 47.37% (stainless steel post). Debonding at the post-cement interface occurred only with isotropic materials. Confocal microscopy observation revealed that gaps were generally associated with an incomplete hybrid layer and reduced resin tags. Regardless of their rigidity, metal and ceramic isotropic posts proved less effective than fiber posts at stabilizing the post and core structure in the absence of the ferrule effect, due to the development of more interfacial defects with either composite or dentin.

  4. In vivo Dentin Caries Model using Rat Molars

    OpenAIRE

    Takashi, HIGASHI; Junji, TAGAMI; Nobuhiro, HANADA; Cariology and Operative Dentistry, Department of Restorative Sciences, Tokyo Medical and Dental University; Cariology and Operative Dentistry, Department of Restorative Sciences, Tokyo Medical and Dental University; Department of Oral Science The National Institute of Infectious Diseases(NIID)

    2000-01-01

    Experimental dentinal caries was induced in rat molars which were inoculated orally with Streptococcus mutans and maintained on a carionenic diet 2000. After 30 days on the diet, the rats were sacrificed. The caries lesions were confirmed with a caries detector, then nanohardness determination of caries dentin were measured with nanoindentation. After hardness measurement, the lesion was examined by SEM and EDS. Dentin caries in sixteen fissures was induced among 20 fissures in the mandibular...

  5. In vivo evaluation of microleakage from composites with new dentine adhesives.

    Science.gov (United States)

    Koliniotou-Koumpia, E; Dionysopoulos, P; Koumpia, E

    2004-10-01

    The purpose of this study was to investigate microleakage in V class composite restorations with total etch versus self-etching adhesives. Experiments were conducted in vivo and were also evaluated interfacial micromorphology. Forty class V cavities were prepared on the buccal surfaces of teeth in three healthy dogs. The cavities were randomly assigned into five groups of eight teeth and restored with one of the following adhesive systems: Etch and Prime 3.0/Definite flow/Definite, Prompt L-pop/Filtek flow/P-60, Admira bond/Admira Flow /Admira, Bond 1/Flow it/Alert, Stae/Wave/Glacier. After 60 days the animals were killed and the teeth were extracted. The teeth were then immersed in a solution of 0.5% basic fuchsin dye for 24 h, sectioned and scored for microleakage. Features of the tooth/restoration interfaces were also examined using Scanning electron microscopy. Student-Newman-Keuls and one-way anova revealed significance differences among the groups for overall wall scores (P < 0.05). The total-etch adhesives revealed significantly less microleakage scores than the self-etching adhesive systems tested. Wilcoxon Signed Ranks Test revealed significant differences between the occlusal and gingival microleakage scores for each adhesive restorative system, with the exception of Admira (P < 0.05).

  6. Effect of Enamel and Dentin Irradiation by Gamma Rays on the Bond Strength of Two Adhesive Restorations

    International Nuclear Information System (INIS)

    Zein, N.S.A.

    2014-01-01

    and neck cancer patients subjected to radiotherapy represents a challenge for restorative dentistry. This is due to the high rates of caries found and the reduction in restoration longevity by restorative material deterioration and failure events that tend to occur rapidly(7). The use of adhesive restorative materials is recommended for the treatment of radiation caries. It has been reported that these tooth-colored restorations survive longer when they are provided with a good seal and protection against secondary caries (8). Unfortunately, the influence of radiotherapy, alone or followed by application of a desensitizing agent, on the bond strengths of these restorations has not been extensively evaluated. Therefore, the current study has been conducted to investigate the effect of enamel and dentin irradiation by gamma rays on the bond strength of two adhesive restorations and to investigate the effect of application of an amorphous calcium phosphate (ACP) desensitizing agent after irradiation and before restoration on the bond strength of the adhesive restorations

  7. Histological evaluation of direct pulp capping of rat pulp with experimentally developed low-viscosity adhesives containing reparative dentin-promoting agents.

    Science.gov (United States)

    Suzuki, Masaya; Taira, Yoshihisa; Kato, Chikage; Shinkai, Koichi; Katoh, Yoshiroh

    2016-01-01

    This study examines the wound healing process in exposed rat pulp when capped with experimental adhesive resin systems. Experimental adhesive resin system for direct pulp capping was composed of primer-I (PI), -II (PII), and -III (PIII) and an experimental bonding agent (EBA). PI was Clearfil(®) SE Bond(®)/Primer (CSP) containing 5.0 wt% CaCl2, PII was PI containing 10 wt% nanofiller (Aerosil(®) 380), and PIII was CSP containing 5.0 wt% of compounds of equal moles of synthetic peptides (pA and pB) derived from dentin matrix protein 1. EBA was Clearfil(®) SE Bond(®)/Bond (CSB) containing 10 wt% hydroxyapatite powders. Three experimental groups were designed. PI was assigned to experimental Groups 1 and 3. PII was assigned to experimental Groups 2 and 3. PIII and EBA were assigned to all experimental adhesive groups. Control teeth were capped with calcium hydroxide preparation (Dycal(®)), and CSP and CSB were applied to the cavity. The rats were sacrificed after each observation period (14, 28, 56, and 112 days). The following parameters were evaluated: pulp tissue disorganization, inflammatory cell infiltration, reparative dentin formation (RDF), and bacterial penetration. There were no significant differences among all the groups for all parameters and all observation periods (p>0.05, Kruskal-Wallis test). All groups showed initial RDF at 14 days postoperatively and extensive RDF until 112 days postoperatively. Groups 2 and 3 demonstrated higher quantity of mineralized dentin bridge formation compared with Group 1. Addition of nanofillers to the primer was effective in promoting high-density RDF. Experimentally developed adhesive resin systems induce the exposed pulp to produce almost the same quantity of reparative dentin as calcium hydroxide. However, we need further studies to elucidate whether the same results could be obtained in humans. Copyright © 2015 Elsevier Ltd. All rights reserved.

  8. Modeling of Sylgard Adhesive Strength

    Energy Technology Data Exchange (ETDEWEB)

    Stevens, Ralph Robert [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2015-02-03

    Sylgard is the name of a silicone elastomeric potting material manufactured by Dow Corning Corporation.1 Although the manufacturer cites its low adhesive strength as a feature of this product, thin layers of Sylgard do in fact have a non-negligible strength, which has been measured in recent tensile and shear debonding tests. The adhesive strength of thin layers of Sylgard potting material can be important in applications in which components having signi cantly di erent thermal expansion properties are potted together, and the potted assembly is subjected to temperature changes. The tensile and shear tractions developed on the potted surfaces of the components can cause signi cant internal stresses, particularly for components made of low-strength materials with a high area-to-volume ratio. This report is organized as follows: recent Sylgard debonding tests are rst brie y summarized, with particular attention to the adhesion between Sylgard and PBX 9501, and also between Sylgard and aluminum. Next, the type of numerical model that will be used to simulate the debonding behavior exhibited in these tests is described. Then the calibration of the debonding model will be illustrated. Finally, the method by which the model parameters are adjusted (scaled) to be applicable to other, non- tested bond thicknesses is summarized, and all parameters of the model (scaled and unscaled) are presented so that other investigators can reproduce all of the simulations described in this report as well as simulations of the application of interest.

  9. Evaluation of microtensile bond strength of total-etch, self-etch, and glass ionomer adhesive to human dentin: An in vitro study

    Directory of Open Access Journals (Sweden)

    Neelima Lakshmi

    2008-01-01

    Full Text Available Aim: To evaluate the microtensile bond strength of Single Bond, AdheSE, and Fuji Bond LC to human dentin. Fifteen non-carious third molars were selected for the study. The teeth were randomly divided into three groups of five teeth each. Each group was given a different bonding treatment. Group I was treated with Single Bond (3M, ESPE, group II with AdheSE (Ivoclar, Vivadent, and group III was treated with Fuji Bond LC (GC America. A T-band metal matrix was placed and composite resin bonded on to the tooth surface using appropriate bonding agents. The composite resin was packed in increments and light cured. Each tooth was sectioned to obtain 1 mm x 1 mm beams of dentin-resin samples. Tensile bond testing was done using a universal testing machine (Instron at a cross-head speed of 0.5 mm/min. Results: The mean bond strength of Single Bond (35.5 MPa was significantly higher than that of AdheSE (32.8 MPa and Fuji Bond LC (32.6 MPa. The difference between the microtensile bond strength values of AdheSE and Fuji Bond LC was statistically insignificant. Inference: Though the bond strength of AdheSE and Fuji Bond LC was above 30 MPa, it was less than that of Single Bond as evaluated by testing of microtensile bond strength.

  10. Characterization of Dentine to Assess Bond Strength of Dental Composites

    Directory of Open Access Journals (Sweden)

    Saad Liaqat

    2015-04-01

    Full Text Available This study was performed to develop alternating dentine adhesion models that could help in the evaluation of a self-bonding dental composite. For this purpose dentine from human and ivory was characterized chemically and microscopically before and after acid etching using Raman and SEM. Mechanical properties of dentine were determined using 3 point bend test. Composite bonding to dentine, with and without use of acid pre-treatment and/or the adhesive, were assessed using a shear bond test. Furthermore, micro gap formation after restoration of 3 mm diameter cavities in dentine was assessed by SEM. Initial hydroxyapatite level in ivory was half that in human dentine. Surface hydroxyapatites decreased by approximately half with every 23 s of acid etch. The human dentine strength (56 MPa was approximately double that of ivory, while the modulus was almost comparable to that of ivory. With adhesive use, average shear bond strengths were 30 and 26 MPa with and without acid etching. With no adhesive, average bond strength was 6 MPa for conventional composites. This, however, increased to 14 MPa with a commercial flowable “self–bonding” composite or upon addition of low levels of an acidic monomer to the experimental composite. The acidic monomer additionally reduced micro-gap formation with the experimental composite. Improved bonding and mechanical properties should reduce composite failures due to recurrent caries or fracture respectively.

  11. Adhesion of Porphyromonas gingivalis and Tannerella forsythia to dentin and titanium with sandblasted and acid etched surface coated with serum and serum proteins - An in vitro study.

    Science.gov (United States)

    Eick, Sigrun; Kindblom, Christian; Mizgalska, Danuta; Magdoń, Anna; Jurczyk, Karolina; Sculean, Anton; Stavropoulos, Andreas

    2017-03-01

    To evaluate the adhesion of selected bacterial strains incl. expression of important virulence factors at dentin and titanium SLA surfaces coated with layers of serum proteins. Dentin- and moderately rough SLA titanium-discs were coated overnight with human serum, or IgG, or human serum albumin (HSA). Thereafter, Porphyromonas gingivalis, Tannerella forsythia, or a six-species mixture were added for 4h and 24h. The number of adhered bacteria (colony forming units; CFU) was determined. Arg-gingipain activity of P. gingivalis and mRNA expressions of P. gingivalis and T. forsythia proteases and T. forsythia protease inhibitor were measured. Coating specimens never resulted in differences exceeding 1.1 log10 CFU, comparing to controls, irrespective the substrate. Counts of T. forsythia were statistically significantly higher at titanium than dentin, the difference was up to 3.7 log10 CFU after 24h (p=0.002). No statistically significant variation regarding adhesion of the mixed culture was detected between surfaces or among coatings. Arg-gingipain activity of P. gingivalis was associated with log10 CFU but not with the surface or the coating. Titanium negatively influenced mRNA expression of T. forsythia protease inhibitor at 24h (p=0.026 uncoated, p=0.009 with serum). The present findings indicate that: a) single bacterial species (T. forsythia) can adhere more readily to titanium SLA than to dentin, b) low expression of T. forsythia protease inhibitor may influence the virulence of the species on titanium SLA surfaces in comparison with teeth, and c) surface properties (e.g. material and/or protein layers) do not appear to significantly influence multi-species adhesion. Copyright © 2016 Elsevier Ltd. All rights reserved.

  12. The mechanical properties of human dentin for 3-D finite element modeling: Numerical and analytical evaluation.

    Science.gov (United States)

    Grzebieluch, Wojciech; Będziński, Romuald; Czapliński, Tomasz; Kaczmarek, Urszula

    2017-07-01

    The FEM is often used in investigations of dentin loading conditions; however, its anisotropy is mostly neglected. The purpose of the study was to evaluate the anisotropy and the elastic properties of an equivalent homogenous material model of human dentin as well as to compare isotropic and anisotropic dentin FE-models. Analytical and numerical dentin homogenization according to Luciano and Barbero was performed and E-modulus (E), Poisson's ratios (v) G-modulus (G) were calculated. The E-modulus of the dentin matrix was 28.0 GPa, Poisson's ratio (v) was 0.3; finite element models of orthotropic and isotropic dentin were created, loaded and compared using Ansys® 14.5 and CodeAster® 11.2 software. Anisotropy of the dentin ranged from 6.9 to 35.2%. E-modulus and G-modulus were as follows: E1 = 22.0-26.0 GPa, E2/E3 = 15.7-23.0 GPa; G12/G13 = 6.96-9.35 GPa and G23 = 6.08-8.09 GPa (highest values in the superficial layer). In FEM analysis of the displacement values were higher in the isotropic than in the orthotropic model, reaching up to 16% by shear load, 37% by compression and 23% in the case of shear with bending. Strain values were higher in the isotropic model, up to 35% for the shear load, 31% for compression and 35% in the case of shear with bending. The decrease in the volumetric fraction and diameter of tubules increased the G and E values. Anisotropy of the dentin applied during FEM analysis decreased the displacements and strain values. The numerical and analytical homogenization of dentin showed similar results.

  13. Comparative evaluation of shear bond strength of two self-etching adhesives (sixth and seventh generation on dentin of primary and permanent teeth: An in vitro study

    Directory of Open Access Journals (Sweden)

    Yaseen S

    2009-03-01

    Full Text Available Aim: The present study was undertaken to compare and evaluate shear bond strength of two self-etching adhesives (sixth and seventh generation on dentin of primary and permanent teeth. Materials and Methods: Flat dentin surface of 64 human anterior teeth (32 primary and 32 permanent divided into four groups of 16 each. Groups A and C were treated with Contax (sixth generation, while groups B and D were treated with Clearfil S3 (seventh generation. A teflon mold was used to build the composite (Filtek Z-350 cylinders on the dentinal surface of all the specimens. Shear bond strength was tested for all the specimens with an Instron Universal Testing Machine. Data were statistically analyzed using one-way ANOVA for multiple group comparison, followed by student′s unpaired ′t′ test for group-wise comparison. Results: There was no statistically significant difference in shear bond strength among the study groups except that primary teeth bonded with Contax exhibited significantly lesser shear bond strength than permanent teeth bonded with Clearfil S3. Conclusion: This study revealed that Clearfil S3 could be of greater advantage in pediatric dentistry than Contax because of its fewer steps and better shear bond strength in dentin of both primary and permanent teeth.

  14. Evaluation of bond strength of silorane and methacrylate based restorative systems to dentin using different cavity models

    Directory of Open Access Journals (Sweden)

    Stephano Zerlottini Isaac

    2013-09-01

    Full Text Available OBJECTIVE: The aim of this in vitro study was to evaluate the microtensile bond strength (µTBS to dentin of two different restorative systems: silorane-based (P90, and methacrylate-based (P60, using two cavity models. MATERIAL AND METHODS: Occlusal enamel of 40 human third molars was removed to expose flat dentin surface. Class I cavities with 4 mm mesial-distal width, 3 mm buccal-lingual width and 3 mm depth (C-factor=4.5 were prepared in 20 teeth, which were divided into two groups (n=10 restored with P60 and P90, bulk-filled after dentin treatment according to manufacturer's instructions. Flat buccal dentin surfaces were prepared in the 20 remaining teeth (C-factor=0.2 and restored with resin blocks measuring 4x3x3 mm using the two restorative systems (n=10. The teeth were sectioned into samples with area between 0.85 and 1.25 mm2 that were submitted to µTBS testing, using a universal testing machine (EMIC at speed of 0.5 mm/min. Fractured specimens were analyzed under stereomicroscope and categorized according to fracture pattern. Data were analyzed using ANOVA and Tukey Kramer tests. RESULTS: For flat surfaces, P60 obtained higher bond strength values compared with P90. However, for Class I cavities, P60 showed significant reduction in bond strength (p0.05, or between Class I Cavity and Flat Surface group, considering P90 restorative system (p>0.05. Regarding fracture pattern, there was no statistical difference among groups (p=0.0713 and 56.3% of the fractures were adhesive. CONCLUSION: It was concluded that methacrylate-based composite µTBS was influenced by cavity models, and the use of silorane-based composite led to similar bond strength values compared to the methacrylate-based composite in cavities with high C-factor.

  15. Effect of polymerization mode of two adhesive systems on push-out bond strength of fiber post to different regions of root canal dentin

    Directory of Open Access Journals (Sweden)

    Shahram Farzin Ebrahimi

    2014-01-01

    Full Text Available Background: A few studies have investigated the effect of the activation mode of adhesive systems on bond strength of fiber posts to root canal dentin. This study investigated the push-out bond strengths of a glass fiber post to different root canal regions with the use of two adhesives with light- and dual-cure polymerization modes. Materials and Methods: In this in vitro study, 40 maxillary central incisors were decoronated at cement-enamel junction with 15 ± 1 mm root length. After root canal therapy and post space preparations, they were randomly divided into four groups. Post spaces were treated with four different adhesives: Excite, Excite Dual cure Single Component (DSC, self-etch adhesive (AdheSE, and AdheSE dual-cure. Then the fiber-reinforced composite (FRC post, Postec Plus, was cemented with dual-cure resin cement, Variolink II. The roots were cut into three 2-mm-thick slices. Push-out tests were performed with a universal testing machine at a crosshead speed of 0.5 mm/min. The mode of failures was determined under a stereomicroscope. Data were analyzed by three-way analysis of variance (ANOVA and Tukey test was conducted to compare post hoc with P < 0.05 as the level of significance. Results: The highest bond strength was obtained for AdheSE dual-cure (15.54 ± 6.90 MPa and the lowest was obtained for Excite light-cure (10.07 ± 7.45 MPa and only the bond strength between these two adhesives had significant difference (P = 0.02. Bond strength decreased from the coronal to the apical in all groups and this was significant in Excite (group 1 and AdheSE (group 3 (P < 0.001. In apical regions, bond strength of dual-cure adhesives was significantly higher than light-cure adhesives (P < 0.001. Conclusion: Push-out bond strength of fiber post to different regions of root canal dentin was affected by both adhesive systems and their polymerization modes.

  16. The measurement of enamel and dentine abrasion by tooth whitening products using an in situ model.

    Science.gov (United States)

    Joiner, A; Collins, L Z; Cox, T F; Pickles, M J; Weader, E; Liscombe, C; Holt, J S

    2005-01-01

    To determine the enamel and dentine wear of two whitening toothpastes using an in situ model with ex vivo brushing. Human enamel/dentine (approximately 50:50) blocks (approximately 4 x 4mm) were placed in the upper buccal aspects of full or partial dentures of a group of 25 subjects. Subjects brushed the specimens ex vivo with either a calcium carbonate/perlite or silica containing whitening toothpaste under exaggerated conditions as compared to normal for 30 s, twice per day. Specimens were removed after 4, 8 and 12 weeks and the wear to the enamel and dentine was determined. Enamel wear was determined by change in Knoop indent length and dentine wear was determined from the enamel-dentine step height, measured using optical profilometry. The mean wear after 12 weeks was for enamel 0.27 and 0.19 microns, and for dentine 34.3 and 61.1 microns, for the calcium carbonate/perlite and silica toothpastes respectively. There were no significant differences between products after 12 weeks. The rate of wear was found to decrease throughout the duration of the study. There were no significant differences between the two whitening toothpastes in terms of enamel and dentine wear after 12 weeks brushing.

  17. Comparative bonding ability to dentin of a universal adhesive system and monomer conversion as functions of extended light curing times and storage.

    Science.gov (United States)

    Sampaio, Paula Costa Pinheiro; Kruly, Paula de Castro; Ribeiro, Clara Cabral; Hilgert, Leandro Augusto; Pereira, Patrícia Nóbrega Rodrigues; Scaffa, Polliana Mendes Candia; Di Hipólito, Vinicius; D'Alpino, Paulo Henrique Perlatti; Garcia, Fernanda Cristina Pimentel

    2017-11-01

    The purpose of this in vitro study was to evaluate the bonding ability and monomer conversion of a universal adhesive system applied to dentin as functions of different curing times and storage. The results were compared among a variety of commercial adhesives. Flat superficial dentin surfaces were exposed on human molars and assigned into one of the following adhesives (n = 15): total-etch Adper Single Bond 2 (SB) and Optibond Solo Plus (OS), self-etch Optibond All in One (OA) and Clearfil SE Bond (CSE), and Scotchbond Universal Adhesive in self-etch mode (SU). The adhesives were applied following the manufacturers' instructions and cured for 10, 20, or 40s. Specimens were processed for the microtensile bond strength (µTBS) test in accordance with the non-trimming technique and tested after 24h and 2 years. The fractured specimens were classified under scanning electron microscopy (SEM). Infrared (IR) spectra were obtained and monomer conversion (%) was calculated by comparing the aliphatic-to-aromatic IR absorption peak ratio before and after polymerization (n=5). Data were analyzed by 2-way ANOVA/Tukey's tests (α = 0.05). At 24-h evaluation, OA and CSE presented similar bond strength means irrespective of the curing time, whereas SB and SU exhibited significantly higher means when cured for 40s as did OS when cured for 20 or 40s (p storage, only the self-etching adhesive Optibond All-In-One exhibited the same bonding ability when cured for longer periods of time. Copyright © 2017 Elsevier Ltd. All rights reserved.

  18. Effect of Water Storage on the Micro-shear Bond Strength of Two Self-etch Adhesives to Enamel and Dentin

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    Z. Jaberi Ansari

    2007-06-01

    Full Text Available Objective: This study evaluated the influence of storage time on micro-shear bond strength of two self-etching materials to enamel and dentin.Materials and Methods: Human third molar teeth were sectioned to 1.5 mm thick beams and randomly divided into 2 groups. In group I, SE Bond and in group II, Tri-S Bond was used to bond a composite rod (AP-X to each treated surface. Specimens were prepared according to manufacturer instructions. Each group was further divided into three subgroups according to water storage time; 1 day, 6 and 12 months. Microshear bond strengths were determined under a crosshead speed of 1mm/min using a universal testing machine and expressed in MPa. Data was statistically analyzed by ANOVA and Dunnett post hoc test.Results: Micro-shear bond strength of two adhesives to enamel and dentin showed a slight but not significant decrease over time (P>0.5. After one day, the mean bond strength of enamel in groups I and II were 39.47 and 34.65 MPa and in dentin were 45.20 and 36.0 MPa respectively. There was no statistically significant differencebetween two materials (P=0.190, P=0.082. After six months the bond strength in group I and II was 35.93 and 35.18 MPa for enamel, and 38.27and 35.19 MPa for dentin respectively, these differences was not statistically significant (P=0.520, P=0.179.After one year, the bond strength of enamel in groups I and II, were 34.47and 29.91MPa, and in dentin were 33.86 and 32.53 MPa respectively which was not statistically significant (P=0.609, P=0.991.Conclusion: The micro-shear bond strength of both adhesives to enamel and dentin decreased slightly over time; however these decreases were not statistically significant.

  19. The effect of warm air-blowing on the microtensile bond strength of one-step self-etch adhesives to root canal dentin.

    Science.gov (United States)

    Taguchi, Keita; Hosaka, Keiichi; Ikeda, Masaomi; Kishikawa, Ryuzo; Foxton, Richard; Nakajima, Masatoshi; Tagami, Junji

    2018-02-01

    The use of warm air-blowing to evaporate solvents of one-step self-etch adhesive systems (1-SEAs) has been reported to be a useful method. The purpose of this study was to evaluate the effect of warm air-blowing on root canal dentin. Four 1-SEAs (Clearfil Bond SE ONE, Unifil Core EM self-etch bond, Estelink, BeautiDualbond EX) were used. Each 1-SEA was applied to root canal dentin according to the manufacturers' instructions. After the adhesives were applied, solvent was evaporated using either normal air (23±1°C) or warm air (80±1°C) for 20s, and resin composite was placed in the post spaces. The air from the dryer, which could be used in normal- or hot-air-mode, was applied at a distance of 5cm above the root canal cavity in the direction of tooth axis. The temperature of the stream of air from the dryer in the hot-air-mode was 80±1°C, and in the normal mode, 23±1°C. After water storage of the specimens for 24h, the μTBS were evaluated at the coronal and apical regions. The μTBSs were statistically analyzed using three-way ANOVA and Student's t-test with Bonferroni correction (α=0.05). The warm air-blowing significantly increased the μTBS of all 1-SEAs at the apical regions, and also significantly increased the μTBS of two adhesives (Estelink and BeautiDualBond EX) at coronal regions. The μTBS of 1-SEAs to root canal dentin was improved by using warm air-blowing. Copyright © 2017 Japan Prosthodontic Society. Published by Elsevier Ltd. All rights reserved.

  20. Conventional dual-cure versus self-adhesive resin cements in dentin bond integrity

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    Renata Andreza Talaveira da Silva

    2011-08-01

    Full Text Available During post preparation, the root canal is exposed to the oral cavity, and endodontic treatment may fail because of coronal leakage, bacterial infection and sealing inability of the luting cement. OBJECTIVE: this study quantified the interfacial continuity produced with conventional dual-cure and self-adhesive resin cements in the cervical (C, medium (M and apical (A thirds of the root. MATERIAL AND METHODS: Forty single-rooted human teeth were restored using Reforpost # 01 conical glass-fiber posts and different materials (N=10 per group: group AC=Adper™ ScotchBond™ Multi-purpose Plus + AllCem; group ARC=Adper™ ScotchBond™ Multi-purpose Plus + RelyX ARC; group U100=RelyX U100; and group MXC=Maxcem Elite. After being kept in 100% humidity at 37°C for 72 hours, the samples were sectioned parallel to their longitudinal axis and positive epoxy resin replicas were made. The scanning electron micrographs of each third section of the teeth were combined using Image Analyst software and measured with AutoCAD-2002. We obtained percentage values of the interfacial continuity. RESULTS: Interfacial continuity was similar in the apical, medium and cervical thirds of the roots within the groups (Friedman test, p>0.05. Comparison of the different cements in a same root third showed that interfacial continuity was lower in MXC (C=45.5%; M=48.5%; A=47.3% than in AC (C=85.9%, M=81.8% and A=76.0%, ARC (C=83.8%, M=82.4% and A=75.0% and U100 (C=84.1%, M=82.4% and A=77.3% (Kruskal-Wallis test, p<0.05. CONCLUSIONS: Allcem, Rely X ARC and U100 provide the best cementation; cementation was similar among root portions; in practical terms, U100 is the best resin because it combines good cementation and easy application and none of the cements provides complete interfacial continuity.

  1. Conventional dual-cure versus self-adhesive resin cements in dentin bond integrity

    Science.gov (United States)

    da SILVA, Renata Andreza Talaveira; COUTINHO, Margareth; CARDOZO, Pedro Igor; da SILVA, Larissa Alves; ZORZATTO, José Roberto

    2011-01-01

    During post preparation, the root canal is exposed to the oral cavity, and endodontic treatment may fail because of coronal leakage, bacterial infection and sealing inability of the luting cement. Objective this study quantified the interfacial continuity produced with conventional dual-cure and self-adhesive resin cements in the cervical (C), medium (M) and apical (A) thirds of the root. Material and methods Forty single-rooted human teeth were restored using Reforpost # 01 conical glass-fiber posts and different materials (N=10 per group): group AC=Adper™ ScotchBond™ Multi-purpose Plus + AllCem; group ARC=Adper™ ScotchBond™ Multi-purpose Plus + RelyX ARC; group U100=RelyX U100; and group MXC=Maxcem Elite. After being kept in 100% humidity at 37ºC for 72 hours, the samples were sectioned parallel to their longitudinal axis and positive epoxy resin replicas were made. The scanning electron micrographs of each third section of the teeth were combined using Image Analyst software and measured with AutoCAD-2002. We obtained percentage values of the interfacial continuity. Results Interfacial continuity was similar in the apical, medium and cervical thirds of the roots within the groups (Friedman test, p>0.05). Comparison of the different cements in a same root third showed that interfacial continuity was lower in MXC (C=45.5%; M=48.5%; A=47.3%) than in AC (C=85.9%, M=81.8% and A=76.0%), ARC (C=83.8%, M=82.4% and A=75.0%) and U100 (C=84.1%, M=82.4% and A=77.3%) (Kruskal-Wallis test, p<0.05). Conclusions Allcem, Rely X ARC and U100 provide the best cementation; cementation was similar among root portions; in practical terms, U100 is the best resin because it combines good cementation and easy application and none of the cements provides complete interfacial continuity. PMID:21710099

  2. Liquid nitrogen-treated autogenous dentin as bone substitute: an experimental study in a rabbit model.

    Science.gov (United States)

    Atiya, Basim K; Shanmuhasuntharam, Palasuntharam; Huat, Siar; Abdulrazzak, Shurooq; Oon, Ha

    2014-01-01

    Different forms of dentin, including untreated, undemineralized, demineralized, boiled, or mixed with other materials, have been evaluated for efficacy as bone substitutes. However, the effects of application of liquid nitrogen-treated dentin for bone grafting remain unknown. The objective of this study was to chronologically evaluate bone healing following grafting with liquid nitrogen-treated dentin in a rabbit model. Autogenous dentin treated with liquid nitrogen at -196°C for 20 minutes was used. In 16 New Zealand White rabbits, a bone defect (5 mm in diameter) was created in each femur and randomly grafted with either autogenous dentin (experimental group) or autogenous bone grafts (positive control). In another four rabbits (negative control), a similar defect in each femur was left empty. The rabbits were sacrificed at 2, 4, 8, and 12 weeks. Explants of grafted sites were harvested for histologic and histomorphometric analysis. At 2 and 4 weeks in both the experimental and positive control groups, accelerated formation of new bone was observed, which was undergoing remodeling at 8 and 12 weeks. The mean new bone score was higher in the experimental than in the negative control groups, but this was not statistically significant. The present results demonstrated that liquid nitrogen-treated autogenous dentin has both osteoconductive and osteoinductive properties and therefore has potential as a bone substitute.

  3. Tensile bond strength of different adhesive systems to primary dentin treated by Er:YAG laser and conventional high-speed drill

    Science.gov (United States)

    Marques, Barbara A.; Navarro, Ricardo S.; Silvestre, Fellipe D.; Pinheiro, Sergio L.; Freitas, Patricia M.; Imparato, Jose Carlos P.; Oda, Margareth

    2005-03-01

    The aim of this study was to evaluate the tensile strength of different adhesive systems to primary tooth dentin prepared by high-speed drill and Er:YAG laser (2.94μm). Buccal surfaces of 38 primary canines were ground and flattened with sand paper disks (#120-600 grit) and distributed into five groups (n=15): G1: diamond bur in high-speed drill (HD)+ 35% phosphoric acid (PA)+Single Bond (SB); G2: HD+self-etching One Up Bond F (OUB);G3: Er:YAG laser (KaVo 3- LELO-FOUSP)(4Hz, 80mJ, 25,72J/cm2) (L)+PA+SB, G4: L+SB, G5: L+OUB. The inverted truncated cone samples built with Z-100 composite resin after storage in water (37°C/24h) were submitted to tensile bond strength test on Mini Instron 4442 (0.5mm/min, 500N). The data were analyzed with ANOVA and Tukey Test (pHD+PA+SB and HD+OUB (p=0.000), L+SB showed higher values than L+PA+SB and L+OUB (p=0.0311). Er:YAG laser radiation promoted significant increase of bond strength of different adhesive systems evaluated in the dentin of primary teeth.

  4. Thin and thick layers of resin-based sealer cement bonded to root dentine compared: Adhesive behaviour.

    Science.gov (United States)

    Pane, Epita S; Palamara, Joseph E A; Messer, Harold H

    2015-12-01

    This study aims to evaluate tensile and shear bond strengths of one epoxy (AH) and two methacrylate resin-based sealers (EZ and RS) in thin and thick layers bonded to root dentine. An alignment device was prepared for accurate positioning of 20 root dentine cylinders in a predefined gap of 0.1 or 1 mm. Sealer was placed in the interface. Bond strength tests were conducted. Mode of failures and representative surfaces were evaluated. Data were analysed using anova and post-hoc tests, with P thick layer of sealer produced higher bond strength, except for the shear bond strength of EZ. Significant differences between thin and thick layers were found only in tensile bond strengths of AH and RS. Mixed type of failure was constantly found with all sealers. Bond strengths of thick layers of resin-based sealers to root dentine tended to be higher than with thin layers. © 2015 Australian Society of Endodontology.

  5. Measurements and modelling of the influence of dentine colour and enamel on tooth colour.

    Science.gov (United States)

    Battersby, Paul D; Battersby, Stephen J

    2015-03-01

    We provide a quantitative predictive model for the extent to which coloured dentine, visible through the enamel, contributes to tooth colour. Our model uses (L(*),a(*),b(*)) measurements rather than spectral measurements. We have used a model system, composed of a slice of bovine enamel placed on top of coloured paper. We have measured the colour of the enamel-paper combination, as an analogue for a tooth, and have related this to the colour of the paper, as an analogue for dentine. By changing the paper colour, we have been able to explore how the colour of dentine determines tooth colour, according to our model system. We have also compared hydrated and desiccated samples. In qualitative terms, superimposing the enamel on top of the paper increases the "lightness" for all colours tested except white while simultaneously reducing the chromaticity, a measure of the extent to which the colour differs from grey. Desiccated enamel is much more effective at increasing the lightness and reducing the chromaticity than hydrated enamel. Quantitatively, our measurements are reproduced by the mathematical model we have developed to within 2% in "lightness" and about 8% in chromaticity. We are able to predict the colour of an analogue for a tooth, composed of bovine enamel and coloured paper, from the colour of an analogue for the dentine, the coloured paper alone, with good accuracy. This understanding provides insights into the role of dentine colour in determining tooth colour. Our work helps quantify the importance of dentine colour, compared to other, extrinsic causes of colour, such as staining, in determining the visible colour of teeth. Our predicted colours represent a baseline to which extrinsic sources will add. Copyright © 2015. Published by Elsevier Ltd.

  6. Effect of chlorhexidine on bonding durability of two self-etching adhesives with and without antibacterial agent to dentin

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    Fereshteh Shafiei

    2013-01-01

    Conclusion: Chlorhexidine was capable of diminishing the loss of BS of these adhesives over time. However, considering the negative effect of chlorhexidine on the initial BS, the benefits of chlorhexidine associated with these adhesives cannot possibly be used.

  7. Resistência adesiva de resinas compostas à dentina Adhesive strength between composite resin and dentin substract

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    Joel BIANCHI

    1999-01-01

    Full Text Available As pesquisas relacionadas com adesão à dentina, em virtude da ampla aplicabilidade, são de grande interesse para a Odontologia. O objetivo deste estudo foi avaliar, através de ensaios de tração, a resistência adesiva ao substrato dentinário, em função de dois sistemas adesivos (3M e Kerr, dois níveis de rugosidade do substrato dentinário (lixas 220 e 600, duas áreas de colagem (3,14 mm2 - 12,56 mm2, e duas condições de armazenagem (inicial e final. A análise dos resultados evidenciou que: 1 houve diferença estatisticamente significativa entre os dois sistemas adesivos, sendo que o da Kerr apresentou os valores mais elevados de resistência adesiva; 2 diferenças significativas não ficaram evidentes na resistência da adesão entre os dois níveis de rugosidade dentinária; 3 as colagens em áreas menores apresentaram, em nível altamente significativo, maior resistência adesiva; 4 a condição de armazenagem influiu nos resultados dependendo da área e do sistema adesivo.There is a wealthy literature on bonding strength of composites to dentine. One of the reasons for the large number of studies is the continuous introduction of new bonding agents in combination with accumulated knowledge about the dentine substrate conditions. The aim of the present study was to compare the "in vitro" tensile bond strengths of two dentine bonding systems (3M and Kerr applied to different dentine roughness (220 and 600 gritand two sizes of bonding areas (3,14 mm2 and 12,56 mm2. Different storage conditions (initial and final were also considered. One hundred and sixty human teeth were randomly allocated in 4 groups. The Kerr system groups and the smallest bonding area groups showed the highest tensile strengths. The storage condition and the substrate roughness did not display a significant influence on bond strengths.

  8. Hydroxyapatite induces spontaneous polymerization of model self-etch dental adhesives.

    Science.gov (United States)

    Zhang, Ying; Wu, Ningjing; Bai, Xinyan; Xu, Changqi; Liu, Yi; Wang, Yong

    2013-10-01

    The objective of this study is to report for the first time the spontaneous polymerization phenomenon of self-etch dental adhesives induced by hydroxylapatite (HAp). Model self-etch adhesives were prepared by using a monomer mixture of bis[2-(methacryloyloxy)ethyl] phosphate (2MP) with 2-hydroxyethyl methacrylate (HEMA). The initiator system consisted of camphorquinone (CQ, 0.022 mmol/g) and ethyl 4-dimethylaminobenzoate (4E, 0.022-0.088 mmol/g). HAp (2-8 wt.%) was added to the neat model adhesive. In a dark environment, the polymerization was monitored in-situ using ATR/FT-IR, and the mechanical properties of the polymerized adhesives were evaluated using nanoindentation technique. Results indicated that spontaneous polymerization was not observed in the absence of HAp. However, as different amounts of HAp were incorporated into the adhesives, spontaneous polymerization was induced. Higher HAp content led to higher degree of conversion (DC), higher rate of polymerization (RP) and shorter induction period (IP). In addition, higher 4E content also elevated DC and RP and reduced IP of the adhesives. Nanoindentation result suggested that the Young's modulus of the polymerized adhesives showed similar dependence on HAp and 4E contents. In summary, interaction with HAp could induce spontaneous polymerization of the model self-etch adhesives. This result provides important information for understanding the initiation mechanism of the self-etch adhesives, and may be of clinical significance to strengthen the adhesive/dentin interface based on the finding. Copyright © 2013 Elsevier B.V. All rights reserved.

  9. Pull-out bond strength of a self-adhesive resin cement to NaOCl-treated root dentin: effect of antioxidizing agents

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    Maryam Khoroushi

    2014-05-01

    Full Text Available Objectives This study evaluated the effect of three antioxidizing agents on pull-out bond strengths of dentin treated with sodium hypochlorite. Materials and Methods Root canals of 75 single-rooted human teeth were prepared. Fifteen teeth were irrigated with normal saline for a negative control group, and the remaining 60 teeth (groups 2 - 5 with 2.5% NaOCl. The teeth in group 2 served as a positive control. Prior to post cementation, the root canals in groups 3 - 5 were irrigated with three antioxidizing agents including 10% rosmarinic acid (RA, Baridge essence, 10% hesperidin (HPN, Sigma, and 10% sodium ascorbate hydrogel (SA, AppliChem. Seventy-five spreaders (#55, taper .02, Produits Dentaires S.A were coated with silica and silanized with the Rocatec system and ceramic bond. All the prepared spreaders were cemented with a self-adhesive resin cement (Bifix SE, Voco Gmbh in the prepared canals. After storage in distilled water (24 h/37℃, the spreaders were pulled out in a universal testing machine at a crosshead speed of 1.0 mm/min. Pull-out strength values were analyzed by one-way ANOVA and Tukey's HSD test (α = 0.05. Results There were significant differences between study groups (p = 0.016. The highest pull-out strength was related to the SA group. The lowest strength was obtained in the positive control group. Conclusions Irrigation with NaOCl during canal preparation decreased bond strength of resin cement to root dentin. Amongst the antioxidants tested, SA had superior results in reversing the diminishing effect of NaOCl irrigation on the bond strength to root dentin.

  10. Comparing the effect of a desensitizing material and a self-etch adhesive on dentin sensitivity after periodontal surgery: a randomized clinical trial

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    Hila Hajizadeh

    2017-07-01

    Full Text Available Objectives This double-blind randomized placebo-controlled clinical trial evaluated the ability of a desensitizing agent and a self-etch adhesive on cervical dentin sensitivity (CDS after periodontal surgery. Materials and Methods Ninety hypersensitive teeth of 13 subjects were included in the study. After periodontal surgery, the teeth of each posterior sextant treated with one of the following materials: G1: Clearfil S3 Bond (Kuraray Dental, G2: Gluma Desensitizer (Heraeus Kulzer, and G3: placebo (water. The sensitivity was assessed using evaporative stimuli before treatment (baseline, T0, 1 day after treatment (T1, after 1 week (T2, and after 1 month (T3 according to visual analog scale (VAS. Results Following the treatment, all the 3 groups showed significant reduction of CDS in T1 compared to T0. Reduction of CDS between T1 and T2 was observed only in G1 but there was no significant difference between T2 and T3 in this group. Although we observed a significant difference in T3 compared to T1 and T2 in G2 and G3, comparison of treatment groups in each assessment time showed a significant difference only in T3. According to paired comparison, this was due to the difference between G2 and G3. Conclusions Dentin sensitivity following periodontal surgery will decrease spontaneously over time, but treating the sensitive teeth with Gluma Desensitizer and Clearfil S3 Bond can have some benefits.

  11. Two Models of Adhesive Debonding of Sylgard

    Energy Technology Data Exchange (ETDEWEB)

    Stevens, Ralph Robert [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2017-03-14

    This report begins with a brief summary of the range of modeling methods used to simulate adhesive debonding. Then the mechanical simulation of the blister debonding test, and the thermomechanical simulation of the potted hemisphere problem are described. For both simulations, details of the chosen modeling techniques, and the reasons for choosing them (and rejecting alternate modeling approaches) will be discussed.

  12. Influence of dental restorations and mastication loadings on dentine fatigue behaviour: Image-based modelling approach.

    Science.gov (United States)

    Vukicevic, Arso M; Zelic, Ksenija; Jovicic, Gordana; Djuric, Marija; Filipovic, Nenad

    2015-05-01

    The aim of this study was to use Finite Element Analysis (FEA) to estimate the influence of various mastication loads and different tooth treatments (composite restoration and endodontic treatment) on dentine fatigue. The analysis of fatigue behaviour of human dentine in intact and composite restored teeth with root-canal-treatment using FEA and fatigue theory was performed. Dentine fatigue behaviour was analysed in three virtual models: intact, composite-restored and endodontically-treated tooth. Volumetric change during the polymerization of composite was modelled by thermal expansion in a heat transfer analysis. Low and high shrinkage stresses were obtained by varying the linear shrinkage of composite. Mastication forces were applied occlusally with the load of 100, 150 and 200N. Assuming one million cycles, Fatigue Failure Index (FFI) was determined using Goodman's criterion while residual fatigue lifetime assessment was performed using Paris-power law. The analysis of the Goodman diagram gave both maximal allowed crack size and maximal number of cycles for the given stress ratio. The size of cracks was measured on virtual models. For the given conditions, fatigue-failure is not likely to happen neither in the intact tooth nor in treated teeth with low shrinkage stress. In the cases of high shrinkage stress, crack length was much larger than the maximal allowed crack and failure occurred with 150 and 200N loads. The maximal allowed crack size was slightly lower in the tooth with root canal treatment which induced somewhat higher FFI than in the case of tooth with only composite restoration. Main factors that lead to dentine fatigue are levels of occlusal load and polymerization stress. However, root canal treatment has small influence on dentine fatigue. The methodology proposed in this study provides a new insight into the fatigue behaviour of teeth after dental treatments. Furthermore, it estimates maximal allowed crack size and maximal number of cycles for a

  13. Resistência de união à dentina de quatro sistemas adesivos Bond strength of four adhesive systems to dentin

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    Marcela Rocha de Oliveira Carrilho

    2002-09-01

    Full Text Available O objetivo do presente estudo foi avaliar a resistência adesiva de quatro sistemas adesivos, composicionalmente diferentes, aplicados à dentina humana. Doze dentes terceiros molares humanos tiveram o esmalte oclusal removido para exposição de uma superfície plana de dentina, na qual foram realizados os procedimentos de adesão. Os dentes foram aleatoriamente divididos em quatro grupos, considerando-se o sistema adesivo e a resina composta a serem empregados: Grupo 1 - Single Bond + P60 (SB; Grupo 2 - Bond 1 + Surefil (B1; Grupo 3 - Prime & Bond NT + Alert (NT e Grupo 4 - Prime & Bond 2.1 + TPH (2.1. Após 24 h de armazenagem em água destilada a 37ºC, os dentes foram seccionados, longitudinalmente, em cortes perpendiculares entre si, para que fossem obtidos espécimes em formato de um paralelogramo com secção transversal retangular de 0,8 mm² de área e 10 mm de comprimento, em média. Os espécimes foram submetidos ao teste de microtração. A análise de variância (alfa = 0,05 demonstrou não haver diferença significante entre os valores médios de resistência obtidos pelos quatro adesivos, embora a análise dos espécimes que sofreram fratura precoce tenha evidenciado menor sensibilidade para o sistema SB.The purpose of the present study was to evaluate the bond strength of four adhesive systems to dentin. Twelve human third molars had their occlusal enamel removed in order to expose a flat dentinal surface, on which the adhesive procedures were carried out. The teeth were divided into four groups, according to the employed adhesive system and composite resin: Group 1 - Single Bond + P60 (SB; Group 2 - Bond 1 + Surefil (B1; Group 3 - Prime & Bond NT + Alert (NT; and Group 4 - Prime & Bond 2.1 + TPH (2.1. After 24 h in distilled water at 37ºC, the teeth were longitudinally sectioned in two perpendicular directions in order to obtain parallelogram-shaped specimens with a cross-sectional area of 0.8 mm² and 10 mm of length, on the

  14. Regulation of Reactionary Dentine Formation.

    Science.gov (United States)

    Neves, V C M; Sharpe, P T

    2018-04-01

    During the treatment of dental caries that has not penetrated the tooth pulp, maintenance of as much unaffected dentine as possible is a major goal during the physical removal of decayed mineral. Damage to dentine leads to release of fossilized factors (transforming growth factor-β [TGF-β] and bone morphogenic protein [BMP]) in the dentine that are believed to stimulate odontoblasts to secrete new "tertiary" dentine (reactionary dentine). This is formed on the pulpal surface of existing dentine and rethickens the dentine. We have previously shown that activation of Wnt/β-catenin signaling is pivotal for tooth repair in exposed pulp injury, and the pathway can be activated by small-molecule GSK-3 antagonists, resulting in enhanced reparative dentine formation. Here, we use a nonexposed pulp injury model to investigate the mechanisms of reactionary dentine formation in vivo, using small molecules to modulate the Wnt/β-catenin, TGF-β, and BMP pathways. We found that a local increase of Wnt activation at the injury site enhances reactionary dentine secretion. In addition, inhibition of TGF-β, BMP, or Wnt pathways does not impede reactionary dentine formation, although inhibition of TGF-β and/or BMP signaling does result in more disorganized, nontubular reactionary dentine. This suggests that Wnt/β-catenin signaling plays no major role in the formation of reactionary dentine, but in common with reparative dentine formation, exogenous elevation of Wnt/β-catenin signaling can enhance tertiary dentine formation. Release of latent TGF-β or BMPs from dentine is not required for the deposition of mineral to form reactionary dentine but does play a role in its organization.

  15. In vitro evaluation of marginal microleakage in class V restorations with composite resin in bovine teeth. Laser irradiation influences and the adhesive system in the dentin pre-treatment

    International Nuclear Information System (INIS)

    Carvalho, Wendell Lima de

    2003-01-01

    Microleakage is one of the most important reasons to restorations failure, it is the responsible for marginal colors changing, new caries, hipersensibility and pulpar diseases. Several techniques and materials have been studied to eliminate or, at least, to decrease microleakage. The cavities preparation with Er:YAG laser and autoconditioning adhesive are some of these techniques and materials. This research has the objective to compare, in vitro, microleakage in class V cavities, prepared with high rotation (conventional treatment), Er:YAG laser (Enamel-400 mj/2 Hz/128,38 J/Cm 2 , Dentin 250 mJ/ 2 Hz/ 80,24 J/Cm 2 ) and the treatment made at dentin with autoconditioning adhesive (Clerafil SE Bond) using Er:YAG laser (with water or not water) or not using Er:YAG laser. It was used 48 bovines teeth with cavities prepared in vestibular face and gingival wall on cement enamel junction and oclusal wall on enamel. The materials used were autoconditioning adhesive (Clerafil SE Bond) and composite resin Z250. Teeth were divided into four groups of twelve samples each one, according to dentin treatment. Group 1 - Conventional cavity and autoconditioning adhesive. Group 2- Cavity prepared with Er: YAG laser and autoconditioning adhesive. Group 3 - Cavity prepared with Er:YAG laser and dentin conditioning with Er:YAG laser associated to water and autoconditioning adhesive. Group 4 - Cavity prepared with Er:YAG laser and dentin conditioning with Er: YAG laser without water and associated to autoconditioning adhesive. Teeth were restored and stocked at 37 deg C, thermocycled and placed into a 50% silver nitrate solution. Right after, teeth were sliced and evaluated on a stereo microscopic magnifying glass in order to see microleakage degree trying to follow a score from 0 to 3. The findings were submitted to Fisher, Anderson-Darling tests and to the not parametric Sen and Puri test. The results indicated that in gingival edge, the Group 2 showed less microleakage than others

  16. Effect of Cigarette Smoke on Resin Composite Bond Strength to Enamel and Dentin Using Different Adhesive Systems.

    Science.gov (United States)

    Theobaldo, J D; Catelan, A; Rodrigues-Filho, U; Marchi, G M; Lima, Danl; Aguiar, Fhb

    2016-01-01

    To evaluate the microshear bond strength of composite resin restorations in dental blocks with or without exposure to cigarette smoke. Eighty bovine dental blocks were divided into eight groups (n=10) according to the type of adhesive (Scotchbond Multi-Purpose, 3M ESPE, St Paul, MN, USA [SBMP]; Single Bond 2, 3M ESPE [SB]; Clearfil SE Bond, Kuraray Medical Inc, Okayama, Japan [CSEB]; Single Bond Universal, 3M ESPE [SBU]) and exposure to smoke (no exposure; exposure for five days/20 cigarettes per day). The adhesive systems were applied to the tooth structure, and the blocks received a composite restoration made using a matrix of perforated pasta. Data were statistically analyzed using analysis of variance and Tukey test (αadhesive systems (padhesives, but no differences were noted in enamel.

  17. Dentin-Composite Bond Strength Measurement Using the Brazilian Disk Test

    Science.gov (United States)

    Carrera, Carola A.; Chen, Yung-Chung; Li, Yuping; Rudney, Joel; Aparicio, Conrado; Fok, Alex

    2016-01-01

    Objectives This study presents a variant of the Brazilian disk test (BDT) for assessing the bond strength between composite resins and dentin. Methods Dentin-composite disks (φ 5 mm × 2 mm) were prepared using either Z100 or Z250 (3M ESPE) in combination with one of three adhesives, Adper Easy Bond (EB), Adper Scotchbond Multi-Purpose (MP) and Adper Single Bond (SB), and tested under diametral compression. Acoustic emission (AE) and digital image correlation (DIC) were used to monitor debonding of the composite from the dentin ring. A finite element (FE) model was created to calculate the bond strengths using the failure loads. Fracture modes were examined by scanning electron microscopy (SEM). Results Most specimens fractured along the dentin-resin composite interface. DIC and AE confirmed interfacial debonding immediately before fracture of the dentin ring. Results showed that the mean bond strength with EB (14.9±1.9 MPa) was significantly higher than with MP (13.2±2.4 MPa) or SB (12.9±3.0 MPa) (p0.05). Z100 (14.5±2.3 MPa) showed higher bond strength than Z250 (12.7±2.5 MPa) (padhesive failure mode. EB failed mostly at the dentin-adhesive interface, whereas MP at the composite-adhesive interface; specimens with SB failed at the composite-adhesive interface and cohesively in the adhesive. Conclusions The BDT variant showed to be a suitable alternative for measuring the bond strength between dentin and composite, with zero premature failure, reduced variability in the measurements, and consistent failure at the dentin-composite interface. PMID:27395367

  18. Evaluation of Osteoconductive and Osteogenic Potential of a Dentin-Based Bone Substitute Using a Calvarial Defect Model

    Directory of Open Access Journals (Sweden)

    Ibrahim Hussain

    2012-01-01

    Full Text Available The aim of this study was to assess the osteoconductive and osteogenic properties of processed bovine dentin using a robust rabbit calvarial defect model. In total, 16 New Zealand White rabbits were operated to create three circular defects in the calvaria. One defect was left unfilled, one filled with collected autogenous bone, and the third defect was filled with the dentin-based bone substitute. Following surgery and after a healing period of either 1 or 6 weeks, a CT scan was obtained. Following sacrificing, the tissues were processed for histological examination. The CT data showed the density in the area grafted with the dentin-based material was higher than the surrounding bone and the areas grafted with autologous bone after 1 week and 6 weeks of healing. The area left unfilled remained an empty defect after 1 week and 6 weeks. Histological examination of the defects filled with the dentin product after 6 weeks showed soft tissue encapsulation around the dentin particles. It can be concluded that the rabbit calvarial model used in this study is a robust model for the assessment of bone materials. Bovine dentin is a biostable material; however, it may not be suitable for repairing large 4-wall defects.

  19. Implant-based oral rehabilitation of a variant model of type I dentinal dysplasia: A rare case report

    Directory of Open Access Journals (Sweden)

    Sowmya Nettem

    2014-01-01

    Full Text Available Dentin dysplasia is an exceptionally rare, autosomal-dominant, hereditary condition, primarily characterized by defective dentin formation affecting both the deciduous and permanent dentitions. The etiology remains imprecise to date, in spite of the numerous hypotheses put forward and the constant updates on this condition. This case report of type I dentin dysplasia exhibits radiographic findings that are unique and diverse from the classical findings of various subtypes of this disease reported to date. This article also depicts the implant-based oral rehabilitation of the young patient diagnosed with this variant model of dentin dysplasia type I. Early diagnosis and implementation of this preventive and curative therapy is vital for avoiding premature exfoliation of deciduous and permanent dentition and the associated residual ridge resorption, thereby overcoming functional and esthetic deficits and ensuring protection of the remaining dentition from further harm.

  20. Evaluation of the tensile bond strength of an adhesive system self-etching in dentin irradiated with Er:YAG laser; Avaliacao da resistencia a tracao de um sistema adesivo self-etching em dentina irradiada com Er:YAG laser

    Energy Technology Data Exchange (ETDEWEB)

    Mello, Andrea Malluf Dabul de

    2000-07-01

    Since Buonocore (1955), several researchers have been seeking for the best adhesive system and treatment for the enamel and dentin surfaces. The use of the acid has been presented as one of the best techniques of dentin conditioning , because this promotes the removal of the 'smear layer and exhibition of dentinal structure, for a best penetration and micro- retention of the adhesive system. However, some conditioning methods have been appearing in the literature, for the substitution or interaction with the acid substances, as the laser. The objective of this work is to evaluate the tensile bond strength of the adhesive system self-etching' associated to a composed resin, in dentin surfaces conditioned with the Er:YAG laser. For this study, freshly extracted human teeth were used and in each one the dentinal surfaces , which were treated with three sandpapers of different granulations (120,400,600), to obtain a standard of the smear layer, before the irradiation of the laser and of the restoring procedure. After these procedures the specimens were storage in distilled water at 37 deg C for 24 hours. Soon after, they were submitted to the tensile strength test .After analyzing the results, we can concluded that the use of the Er:YAG laser can substitute the drill without the need of conditioning, when using the adhesive system 'self-etching' in the dentinal surfaces because there was a decline in the strength of adhesion in the groups conditioned with the laser. (author)

  1. A Miniature Swine Model for Stem Cell-Based De Novo Regeneration of Dental Pulp and Dentin-Like Tissue.

    Science.gov (United States)

    Zhu, Xiaofei; Liu, Jie; Yu, Zongdong; Chen, Chao-An; Aksel, Hacer; Azim, Adham A; Huang, George T-J

    2018-02-01

    The goal of this study was to establish mini-swine as a large animal model for stem cell-based pulp regeneration studies. Swine dental pulp stem cells (sDPSCs) were isolated from mini-swine and characterized in vitro. For in vivo studies, we first employed both ectopic and semi-orthotopic study models using severe combined immunodeficiency mice. One is hydroxyapatite-tricalcium phosphate (HA/TCP) model for pulp-dentin complex formation, and the other is tooth fragment model for complete pulp regeneration with new dentin depositing along the canal walls. We found that sDPSCs are similar to their human counterparts exhibiting mesenchymal stem cell characteristics with ability to form colony forming unit-fibroblastic and odontogenic differentiation potential. sDPSCs formed pulp-dentin complex in the HA/TCP model and showed pulp regeneration capacity in the tooth fragment model. We then tested orthotopic pulp regeneration on mini-swine including the use of multi-rooted teeth. Using autologous sDPSCs carried by hydrogel and transplanted into the mini-swine root canal space, we observed regeneration of vascularized pulp-like tissue with a layer of newly deposited dentin-like (rD) tissue or osteodentin along the canal walls. In some cases, dentin bridge-like structure was observed. Immunohistochemical analysis detected the expression of nestin, dentin sialophosphoprotein, dentin matrix protein 1, and bone sialoprotein in odontoblast-like cells lining against the produced rD. We also tested the use of allogeneic sDPSCs for the same procedures. Similar findings were observed in allogeneic transplantation. This study is the first to show an establishment of mini-swine as a suitable large animal model utilizing multi-rooted teeth for further cell-based pulp regeneration studies.

  2. Adhesive contact: from atomistic model to continuum model

    International Nuclear Information System (INIS)

    Fan Kang-Qi; Jia Jian-Yuan; Zhu Ying-Min; Zhang Xiu-Yan

    2011-01-01

    Two types of Lennard-Jones potential are widely used in modeling adhesive contacts. However, the relationships between the parameters of the two types of Lennard-Jones potential are not well defined. This paper employs a self-consistent method to derive the Lennard-Jones surface force law from the interatomic Lennard-Jones potential with emphasis on the relationships between the parameters. The effect of using correct parameters in the adhesion models is demonstrated in single sphere-flat contact via continuum models and an atomistic model. Furthermore, the adhesion hysteresis behaviour is investigated, and the S-shaped force-distance relation is revealed by the atomistic model. It shows that the adhesion hysteresis loop is generated by the jump-to-contact and jump-off-contact, which are illustrated by the S-shaped force-distance curve. (atomic and molecular physics)

  3. Adhesion

    Science.gov (United States)

    ... Supplements Videos & Tools Español You Are Here: Home → Medical Encyclopedia → Adhesion URL of this page: //medlineplus.gov/ency/article/001493.htm Adhesion To use the sharing features on this page, please enable JavaScript. Adhesions are bands of scar-like tissue that form between two ...

  4. Understanding Surface Adhesion in Nature: A Peeling Model.

    Science.gov (United States)

    Gu, Zhen; Li, Siheng; Zhang, Feilong; Wang, Shutao

    2016-07-01

    Nature often exhibits various interesting and unique adhesive surfaces. The attempt to understand the natural adhesion phenomena can continuously guide the design of artificial adhesive surfaces by proposing simplified models of surface adhesion. Among those models, a peeling model can often effectively reflect the adhesive property between two surfaces during their attachment and detachment processes. In the context, this review summarizes the recent advances about the peeling model in understanding unique adhesive properties on natural and artificial surfaces. It mainly includes four parts: a brief introduction to natural surface adhesion, the theoretical basis and progress of the peeling model, application of the peeling model, and finally, conclusions. It is believed that this review is helpful to various fields, such as surface engineering, biomedicine, microelectronics, and so on.

  5. Calibration of a lactic-acid model for simulating biofilm-induced degradation of the dentin-composite interface.

    Science.gov (United States)

    Zhu, Laikuan; Li, Yuping; Carrera, Carola A; Chen, Yung-Chung; Li, Mingyu; Fok, Alex

    2017-11-01

    To verify and calibrate a chemical model for simulating the degradation of the dentin-composite interface induced by multi-species oral biofilms in vitro. Dentin-composite disks (5-mm dia.×2-mm thick) were made from bovine incisor roots and filled with either Z100™ (Z100) or Filtek™ LS (LS) composite. The disks, which were covered with nail varnish, but with one of the dentin-composite margins exposed, were immersed in lactic acid solution at pH 4.5 for up to 48h. Diametral compression was performed to measure the reduction in bond strength of the dentin-composite disks following acid challenge. Scanning electron microscopy (SEM) was used to examine decalcification of dentin and fracture modes of the disks. To better understand the degradation process, micro-computed tomography, in combination with a radiopaque dye (AgNO 3 ), was used to assess interfacial leakage in 3D longitudinally, while SEM was used to determine the path of leakage. One-way analysis of variance (ANOVA) was used to analyze the results, with the level of statistical significance set at pcomposite interface, instead. The degree of dentin demineralization, the reduction in debonding load and the modes of failure observed were very similar to those induced by multi-species oral biofilms found in the previous work. Leakage of AgNO 3 occurred mainly along the hybrid layer. The specimens filled with Z100 had a thicker hybrid layer (∼6.5μm), which exhibited more interfacial leakage than those filled with LS. The chemical model with lactic acid used in this study can induce degradation to the dentin-composite interface similar to those produced by multi-species biofilms. With appropriate calibration, this could provide an effective in vitro method for ageing composite restorations in assessing their potential clinical performance. Copyright © 2017 The Academy of Dental Materials. All rights reserved.

  6. Microshear bonding effectiveness of different dentin regions

    Directory of Open Access Journals (Sweden)

    Anelise F Montagner

    2015-01-01

    Full Text Available Aim: The aim of this in vitro study was to evaluate the influence of dentin surfaces with different tubule orientation on bond strength of a multimode adhesive system. Materials and Methods: Twenty human molars were selected and prepared in different ways in order to produce different dentin regions. The teeth were randomized (n = 5 according to the application modes of a multimode adhesive system (etch-and-rinse and self-etching and to the dentin region (occlusal and proximal - 1.5 mm depth from dentinoenamel junction. Cylindrical restorations were performed on dentin with a starch tube. The samples remained immersed in distilled water for 24 h and the microshear bond strength (μSBS test was performed. The μSBS values were expressed in MPa and analyzed with ANOVA and Tukey′s test (P < 0.05. Results: There was no significant difference in the bond strength values between the application modes of the adhesive system (P = 0.19; however, the dentin regions (P < 0.05 significantly affected the μSBS. The proximal dentin presented higher bond strength values than occlusal dentin. Conclusion: The dentin surfaces with different tubule orientation influenced the bonding effectiveness of the adhesive system tested.

  7. In-vitro transdentinal diffusion of monomers from adhesives.

    Science.gov (United States)

    Putzeys, Eveline; Duca, Radu Corneliu; Coppens, Lieve; Vanoirbeek, Jeroen; Godderis, Lode; Van Meerbeek, Bart; Van Landuyt, Kirsten L

    2018-06-01

    Biocompatibility of adhesives is important since adhesives may be applied on dentin near the pulp. Accurate knowledge of the quantity of monomers reaching the pulp is important to determine potential side effects. The aim of this study was to assess the transdentinal diffusion of residual monomers from dental adhesive systems using an in-vitro pulp chamber model. Dentin disks with a thickness of 300 µm were produced from human third molars. These disks were fixed between two open-ended glass tubes, representing an in-vitro pulp chamber. The etch-and-rinse adhesive OptiBond FL and the self-etch adhesive Clearfil SE Bond were applied to the dentin side of the disks, while on in the pulpal side, the glass tube was filled with 600 µL water. The transdentinal diffusion of different monomers was quantified using ultra-performance liquid chromatography-tandem mass spectrometry. The monomers HEMA, CQ, BisGMA, GPDM, 10-MDP and UDMA eluted from the dental materials and were able to diffuse through the dentin disks to a certain extent. Compounds with a lower molecular weight (uncured group: HEMA 7850 nmol and CQ 78.2 nmol) were more likely to elute and diffuse compared to monomers with a higher molecular weight (uncured group: BisGMA 0.42 nmol). When the adhesives were left uncured, diffusion was up to 10 times higher compared to the cured conditions. This in-vitro research resulted in the quantification of various monomers able to diffuse through dentin and therefore contributes to a more detailed understanding about the potential exposure of the dental pulp to monomers from dental adhesives. Biocompatibility of adhesives is important since adhesives may be applied on dentin near the pulp, where tubular density and diameter are greatest. Copyright © 2018. Published by Elsevier Ltd.

  8. Microleakage of two self-adhesive cements in the enamel and dentin after 24 hours and two months.

    Directory of Open Access Journals (Sweden)

    Zahra Jaberi Ansari

    2014-08-01

    Full Text Available Microleakage is a main cause of restorative treatment failure. In this study, we compared occlusal and cervical microleakage of two self-adhesive cements after 24 hours and two months.In this in-vitro experimental study, class II inlay cavities were prepared on 60 sound human third molars. Composite inlays were fabricated with Z100 composite resin. The teeth were randomly assigned to six groups. RelyX-Arc (control, RelyX-Unicem and Maxcem were used for the first three groups and specimens were stored in distilled water at 37°C for 24 hours. The same cements were used for the remaining three groups, but the specimens were stored for 2 months. The teeth were subjected to 500 thermal cycles (5°C and 55°C and immersed in 0.5% basic fuchsin for 24 hours and then sectioned mesiodistally and dye penetration was evaluated in a class II cavity with occlusal and cervical margins using X20 magnification stereomicroscope. Data were analyzed using Kruskal Wallis and Mann-Whitney U tests.After 24 hours, cements had significant differences only in cervical margin microleakage (P=0.0001 and microleakage of RelyX-Unicem and Maxcem was significantly more than that of RelyX-Arc (both P=0.0001. Cervical microleakage in RelyX-Unicem and Maxcem was greater than occlusal (P=0.0001 and P=0.001, respectively. Microleakage was not significantly different between the occlusal and cervical margins after 2 months.Cervical microleakage was greater than occlusal in RelyX-Unicem and Maxcem after 24h. The greatest microleakage was reported for the cervical margin of RelyX-Unicem after 24 hours.

  9. Tensile bond strength of self-etching versus total-etching adhesive systems under different dentinal substrate conditions Resistência de união à tração de sistemas adesivos autocondicionantes versus de condicionamento total, em diferentes condições de substrato dentinário

    Directory of Open Access Journals (Sweden)

    Alexandre Henrique Susin

    2007-03-01

    Full Text Available The use of acid etchants to produce surface demineralization and collagen network exposure, allowing adhesive monomers interdiffusion and consequently the formation of a hybrid layer, has been considered the most efficient mechanism of dentin bonding. The aim of this study was to compare the tensile bond strength to dentin of three adhesive systems, two self-etching ones (Clearfil SE Bond - CSEB and One Up Bond F - OUBF and one total-etching one (Single Bond - SB, under three dentinal substrate conditions (wet, dry and re-wet. Ninety human, freshly extracted third molars were sectioned at the occlusal surface to remove enamel and to form a flat dentin wall. The specimens were restored with composite resin (Filtek Z250 and submitted to tensile bond strength testing (TBS in an MTS 810. The data were submitted to two-way ANOVA and Tukey's test (p = 0.05. Wet dentin presented the highest TBS values for SB and CSEB. Dry dentin and re-wet produced significantly lower TBS values when using SB. OUBF was not affected by the different conditions of the dentin substrate, producing similar TBS values regardless of the surface pretreatments.O uso de condicionadores ácidos para desmineralizar a superfície dental e expor a rede de fibras colágenas para interdifusão dos monômeros adesivos e conseqüente formação da camada híbrida tem sido considerado o mais eficiente mecanismo de adesão dos agentes de união. O objetivo deste estudo foi comparar a resistência de união à dentina de três sistemas adesivos, dois autocondicionantes (Clearfil SE Bond - CSEB e One Up Bond F - OUBF e um de condicionamento total (Single Bond - SB, sob três diferentes condições de substrato dentinário (úmido, seco e reidratado. Noventa terceiros molares humanos recém-extraídos foram cortados na superfície oclusal, para se remover o esmalte e formar uma parede plana de dentina. Os espécimes foram restaurados com resina composta (Filtek Z250 e submetidos ao teste de

  10. Buffering action of human dentin in vitro.

    Science.gov (United States)

    Camps, J; Pashley, D H

    2000-01-01

    The purpose of this study was to determine the relative contributions of the mineral and organic phases of dentin to its total buffering capacity and to compare the buffering abilities of normal and caries-affected dentin for acids used in adhesive dentistry. Disks of normal and caries-affected human coronal dentin 0.6 mm thick were prepared. Fifty microL of various acids were applied to the surface of mineralized or completely demineralized dentin for varying lengths of time. They were collected from the surface and combined with water rinses to permit titration of the total amount of acid applied, the amount recovered, the total amount that was taken up by the dentin, and the amount that diffused across dentin into 1 mL of water. Equal volumes of acids were applied to mineralized or demineralized dentin powder or hydroxyapatite powder. About 88% to 90% of applied acid was recovered from the surface; only 10% to 12% of the acid was taken up by dentin. Of the H+ that was taken up, only 1% to 2% actually diffused across 0.6 mm of dentin. Increasing the application time of 37% phosphoric acid did not increase the amount of H+ that diffused across dentin. Increasing the concentration of phosphoric acid from 10% to 65% produced only slight increases in H+ diffusion across dentin. There was no difference in the buffering capacity of normal vs caries-affected dentin disks. Almost all of the buffering capacity of dentin is due to its mineral phase. The high buffering capacity of dentin and the high reactivity of H+ insure that little H+ diffuses through dentin more than 0.6 mm thick.

  11. Microtensile bond strength and scanning electron microscopic evaluation of zirconia bonded to dentin using two self-adhesive resin cements; effect of airborne abrasion and aging

    Directory of Open Access Journals (Sweden)

    Reem Gamal

    2017-12-01

    Conclusions: Airborne abrasion-surface treatment of zirconia significantly enhanced the μTBS of both cements adhered to dentin while aging had an adverse effect. MS showed higher insignificant μTBS.

  12. Monomer-to-polymer conversion and micro-tensile bond strength to dentine of experimental and commercial adhesives containing diphenyl(2,4,6-trimethylbenzoyl)phosphine oxide or a camphorquinone/amine photo-initiator system.

    Science.gov (United States)

    Miletic, Vesna; Pongprueksa, Pong; De Munck, Jan; Brooks, Neil R; Van Meerbeek, Bart

    2013-10-01

    To compare the degree of conversion (DC) of adhesives initiated by diphenyl(2,4,6-trimethylbenzoyl)phosphine oxide (TPO) or a camphorquinone/tertiary amine system (CQ/Amine) as well as their 'immediate' micro-tensile bond strength (μTBS) to bur-cut dentine. DC of Scotchbond Universal ('SBU'; 3M ESPE), its experimental counterpart containing TPO as photo-initiator system, an experimental G-aenial Bond ('Ga-B'; GC) adhesive formulation, and an experimental LUB-102 adhesive formulation ('LUB', Kuraray Noritake), containing as photo-initiatior system either 2wt% CQ along with 2wt% tertiary amine ('SBU_CQ/Amine'; 'Ga-B_CQ/Amine'; 'LUB_CQ/Amine'), or 2wt% TPO ('SBU_TPO'; 'Ga-B_TPO'; 'LUB_TPO'), was determined using Fourier-transform infrared spectroscopy (FTIR), after being cured with a dual-wavelength light-curing unit (bluephase 20i, Ivoclar Vivadent). The same adhesive formulations were applied to bur-cut mid-coronal dentine of intact human molars, and subjected to a μTBS test after 1-week water storage. Besides being applied following a self-etch (SE) application mode, the adhesive formulations SBU_CQ/Amine and SBU_TPO were also applied following an etch-and-rinse (E&R) mode, this both for DS and μTBS measurement. No significant difference in DC was found for any of the adhesive formulations, except for SBU_CQ/Amine_SE and SBU_TPO_SE. For both SBU formulations, a significantly higher DC was reached for the E&R than the SE approach. Regarding μTBS, no significant differences were recorded, except for the significantly higher μTBS measured for SBU_CQ/Amine_E&R and SBU_TPO_E&R. In self-etch adhesives, the photo-initiator TPO may be used instead of CQ/Amine. The curing and 'immediate' bonding efficiency depended on the application protocol (E&R versus SE), but not on the photo-initiator system. The photo-initiator TPO may be used in self-etch adhesives instead of CQ/Amine with similar curing and 'immediate' bonding efficiency. Copyright © 2013 Elsevier Ltd. All

  13. Bond strength of etch-and-rinse and self-etch adhesive systems to enamel and dentin irradiated with a novel CO2 9.3 μm short-pulsed laser for dental restorative procedures.

    Science.gov (United States)

    Rechmann, Peter; Bartolome, N; Kinsel, R; Vaderhobli, R; Rechmann, B M T

    2017-12-01

    The objective of this study was to evaluate the influence of CO 2 9.3 μm short-pulsed laser irradiation on the shear bond strength of composite resin to enamel and dentin. Two hundred enamel and 210 dentin samples were irradiated with a 9.3 µm carbon dioxide laser (Solea, Convergent Dental, Inc., Natick, MA) with energies which either enhanced caries resistance or were effective for ablation. OptiBond Solo Plus [OptiBondTE] (Kerr Corporation, Orange, CA) and Peak Universal Bond light-cured adhesive [PeakTE] (Ultradent Products, South Jordan, UT) were used. In addition, Scotchbond Universal [ScotchbondSE] (3M ESPE, St. Paul, MN) and Peak SE self-etching primer with Peak Universal Bond light-cured adhesive [PeakSE] (Ultradent Products) were tested. Clearfil APX (Kuraray, New York, NY) was bonded to the samples. After 24 h, a single plane shear bond test was performed. Using the caries preventive setting on enamel resulted in increased shear bond strength for all bonding agents except for self-etch PeakSE. The highest overall bond strength was seen with PeakTE (41.29 ± 6.04 MPa). Etch-and-rinse systems achieved higher bond strength values to ablated enamel than the self-etch systems did. PeakTE showed the highest shear bond strength with 35.22 ± 4.40 MPa. OptiBondTE reached 93.8% of its control value. The self-etch system PeakSE presented significantly lower bond strength. The shear bond strength to dentin ranged between 19.15 ± 3.49 MPa for OptiBondTE and 43.94 ± 6.47 MPa for PeakSE. Etch-and-rinse systems had consistently higher bond strength to CO 2 9.3 µm laser-ablated enamel. Using the maximum recommended energy for dentin ablation, the self-etch system PeakSE reached the highest bond strength (43.9 ± 6.5 MPa).

  14. Reflections about Adhesive Systems

    OpenAIRE

    de Freitas Borges, Marciano; Diesel, Pâmela Gutheil; Corrêa, Fernanda Gomez; Bernardi, Eledana; Fernandes Montagner, Anelise; Skupien, Jovito Adiel; Susin, Alexandre Henrique

    2010-01-01

    The adhesive systems are responsible for an efficient union between teeth and resin, resulting in a longevity restoration. They are organic molecules di or multifunctional that contain reactive groups that interact with dentin and with the resin monomer of composite resin. The adhesive systems are characterized by wet adhesion, which is a result of presence of hidrophylics radicals in their compositions, to promote a better bond and the best properties of the adhesion. Adhesive systems may us...

  15. Effect of immediate and delayed dentin sealing on the fracture strength, failure type and Weilbull characteristics of lithiumdisilicate laminate veneers

    NARCIS (Netherlands)

    Gresnigt, Marco M.M.; Cune, Marco S.; de Roos, Joanne G.; Özcan, Mutlu

    OBJECTIVES: Adhesion on dentin is less reliable than on enamel, which could affect the durability of laminate veneers (LV). Immediate dentin sealing (IDS) is suggested instead of delayed dentin sealing (DDS) to overcome hypersensitivity and prevent debonding from dentin. This study evaluated the

  16. Adhesion of perfume-filled microcapsules to model fabric surfaces.

    Science.gov (United States)

    He, Yanping; Bowen, James; Andrews, James W; Liu, Min; Smets, Johan; Zhang, Zhibing

    2014-01-01

    The retention and adhesion of melamine formaldehyde (MF) microcapsules on a model fabric surface in aqueous solution were investigated using a customised flow chamber technique and atomic force microscopy (AFM). A cellulose film was employed as a model fabric surface. Modification of the cellulose with chitosan was found to increase the retention and adhesion of microcapsules on the model fabric surface. The AFM force-displacement data reveal that bridging forces resulting from the extension of cellulose chains dominate the adhesion between the microcapsule and the unmodified cellulose film, whereas electrostatic attraction helps the microcapsules adhere to the chitosan-modified cellulose film. The correlation between results obtained using these two complementary techniques suggests that the flow chamber device can be potentially used for rapid screening of the effect of chemical modification on the adhesion of microparticles to surfaces, reducing the time required to achieve an optimal formulation.

  17. Attenuation of postoperative adhesions using a modeled manual therapy.

    Directory of Open Access Journals (Sweden)

    Geoffrey M Bove

    Full Text Available Postoperative adhesions are pathological attachments that develop between abdominopelvic structures following surgery. Considered unavoidable and ubiquitous, postoperative adhesions lead to bowel obstructions, infertility, pain, and reoperations. As such, they represent a substantial health care challenge. Despite over a century of research, no preventive treatment exists. We hypothesized that postoperative adhesions develop from a lack of movement of the abdominopelvic organs in the immediate postoperative period while rendered immobile by surgery and opiates, and tested whether manual therapy would prevent their development. In a modified rat cecal abrasion model, rats were allocated to receive treatment with manual therapy or not, and their resulting adhesions were quantified. We also characterized macrophage phenotype. In separate experiments we tested the safety of the treatment on a strictureplasty model, and also the efficacy of the treatment following adhesiolysis. We show that the treatment led to reduced frequency and size of cohesive adhesions, but not other types of adhesions, such as those involving intraperitoneal fatty structures. This effect was associated with a delay in the appearance of trophic macrophages. The treatment did not inhibit healing or induce undesirable complications following strictureplasty. Our results support that that maintained movements of damaged structures in the immediate postoperative period has potential to act as an effective preventive for attenuating cohesive postoperative adhesion development. Our findings lay the groundwork for further research, including mechanical and pharmacologic approaches to maintain movements during healing.

  18. Attenuation of postoperative adhesions using a modeled manual therapy.

    Science.gov (United States)

    Bove, Geoffrey M; Chapelle, Susan L; Hanlon, Katherine E; Diamond, Michael P; Mokler, David J

    2017-01-01

    Postoperative adhesions are pathological attachments that develop between abdominopelvic structures following surgery. Considered unavoidable and ubiquitous, postoperative adhesions lead to bowel obstructions, infertility, pain, and reoperations. As such, they represent a substantial health care challenge. Despite over a century of research, no preventive treatment exists. We hypothesized that postoperative adhesions develop from a lack of movement of the abdominopelvic organs in the immediate postoperative period while rendered immobile by surgery and opiates, and tested whether manual therapy would prevent their development. In a modified rat cecal abrasion model, rats were allocated to receive treatment with manual therapy or not, and their resulting adhesions were quantified. We also characterized macrophage phenotype. In separate experiments we tested the safety of the treatment on a strictureplasty model, and also the efficacy of the treatment following adhesiolysis. We show that the treatment led to reduced frequency and size of cohesive adhesions, but not other types of adhesions, such as those involving intraperitoneal fatty structures. This effect was associated with a delay in the appearance of trophic macrophages. The treatment did not inhibit healing or induce undesirable complications following strictureplasty. Our results support that that maintained movements of damaged structures in the immediate postoperative period has potential to act as an effective preventive for attenuating cohesive postoperative adhesion development. Our findings lay the groundwork for further research, including mechanical and pharmacologic approaches to maintain movements during healing.

  19. Dentin-composite bond strength measurement using the Brazilian disk test.

    Science.gov (United States)

    Carrera, Carola A; Chen, Yung-Chung; Li, Yuping; Rudney, Joel; Aparicio, Conrado; Fok, Alex

    2016-09-01

    This study presents a variant of the Brazilian disk test (BDT) for assessing the bond strength between composite resins and dentin. Dentin-composite disks (ϕ 5mm×2mm) were prepared using either Z100 or Z250 (3M ESPE) in combination with one of three adhesives, Adper Easy Bond (EB), Adper Scotchbond Multi-Purpose (MP) and Adper Single Bond (SB), and tested under diametral compression. Acoustic emission (AE) and digital image correlation (DIC) were used to monitor debonding of the composite from the dentin ring. A finite element (FE) model was created to calculate the bond strengths using the failure loads. Fracture modes were examined by scanning electron microscopy (SEM). Most specimens fractured along the dentin-resin composite interface. DIC and AE confirmed interfacial debonding immediately before fracture of the dentin ring. Results showed that the mean bond strength with EB (14.9±1.9MPa) was significantly higher than with MP (13.2±2.4MPa) or SB (12.9±3.0MPa) (p0.05). Z100 (14.5±2.3MPa) showed higher bond strength than Z250 (12.7±2.5MPa) (pstrength between dentin and composite, with zero premature failure, reduced variability in the measurements, and consistent failure at the dentin-composite interface. The new test could help to predict the clinical performance of adhesive systems more effectively and consistently by reducing the coefficient of variation in the measured bond strength. Copyright © 2016 Elsevier Ltd. All rights reserved.

  20. Bio-reconstruction of root canal using dentin post

    Directory of Open Access Journals (Sweden)

    Navin Mishra

    2013-01-01

    Full Text Available This case report describes the successful management of complicated crown fracture of left maxillary central incisor (#21 of 23-year-old male using dentin (biological post made from human tooth. Endodontic treatment was initiated and sectional obturation was done using ProTaper gutta-percha and AH Plus sealer. The coronal space was modified to receive a human dentin post. A two-step procedure comprising direct and indirect technique of post fabrication was done to achieve the accuracy of biological post. A maxillary cuspid from an institutional tooth bank was taken, sectioned mesiodistally using a diamond disc simulating the length and thickness of mock post. Dentin post was first verified on the plaster model and then cemented in tooth #21. Core build up was done with composite and porcelain fused to metal (PFM crown was luted. On the follow-up visits, patient was asymptomatic and radiographic evaluation revealed normal periradicular architecture. Biological posts may be good alternatives to conventional post systems as they preserve internal dentin walls, provide excellent adhesion, and resilience similar to natural tooth structure.

  1. A Model System for Predicting Drug Penetration thru Decayed Dentin into Inflamed Pulps

    Science.gov (United States)

    1991-01-09

    INTRODUCTION Patients seeking emergency treatment for non-traumatic pain of odonto - genic origin are frequently manifesting the symptoms of "acute" pulpitis...products in this research does not reflect Government endorsement. 21 REFERENCES BRANNSTROM, H. and GARBEROGLIO, R. (1972): The Dentinal Tubules and Odonto

  2. Model of SNARE-mediated membrane adhesion kinetics.

    Directory of Open Access Journals (Sweden)

    Jason M Warner

    Full Text Available SNARE proteins are conserved components of the core fusion machinery driving diverse membrane adhesion and fusion processes in the cell. In many cases micron-sized membranes adhere over large areas before fusion. Reconstituted in vitro assays have helped isolate SNARE mechanisms in small membrane adhesion-fusion and are emerging as powerful tools to study large membrane systems by use of giant unilamellar vesicles (GUVs. Here we model SNARE-mediated adhesion kinetics in SNARE-reconstituted GUV-GUV or GUV-supported bilayer experiments. Adhesion involves many SNAREs whose complexation pulls apposing membranes into contact. The contact region is a tightly bound rapidly expanding patch whose growth velocity v(patch increases with SNARE density Gamma(snare. We find three patch expansion regimes: slow, intermediate, fast. Typical experiments belong to the fast regime where v(patch ~ (Gamma(snare(2/3 depends on SNARE diffusivities and complexation binding constant. The model predicts growth velocities ~10 - 300 microm/s. The patch may provide a close contact region where SNAREs can trigger fusion. Extending the model to a simple description of fusion, a broad distribution of fusion times is predicted. Increasing SNARE density accelerates fusion by boosting the patch growth velocity, thereby providing more complexes to participate in fusion. This quantifies the notion of SNAREs as dual adhesion-fusion agents.

  3. Wood-adhesive bonding failure : modeling and simulation

    Science.gov (United States)

    Zhiyong Cai

    2010-01-01

    The mechanism of wood bonding failure when exposed to wet conditions or wet/dry cycles is not fully understood and the role of the resulting internal stresses exerted upon the wood-adhesive bondline has yet to be quantitatively determined. Unlike previous modeling this study has developed a new two-dimensional internal-stress model on the basis of the mechanics of...

  4. Mechanical and microbiological properties and drug release modeling of an etch-and-rinse adhesive containing copper nanoparticles.

    Science.gov (United States)

    Gutiérrez, M F; Malaquias, P; Matos, T P; Szesz, A; Souza, S; Bermudez, J; Reis, A; Loguercio, A D; Farago, P V

    2017-03-01

    To evaluate the effect of addition of copper nanoparticles (CN) at different concentrations into a two-step etch-and-rinse (2-ER) adhesive on antimicrobial activity (AMA), copper release (CR), ultimate tensile strength (UTS), degree of conversion (DC), water sorption (WS), solubility (SO), as well as the immediate (IM) and 1-year resin-dentin bond strength (μTBS) and nanoleakage (NL). Seven adhesives were formulated according to the addition of CN (0, 0.0075, 0.015, 0.06, 0.1, 0.5 and 1wt%) in adhesive. The AMA was evaluated against Streptococcus mutans using agar diffusion assay. For CR, WS and SO, specimens were constructed and tested for 28 days. For UTS, specimens were tested after 24h and 28 days. For DC, specimens were constructed and tested after 24h by FTIR. After enamel removal, the ER was applied to dentin. After composite resin build-ups, specimens were sectioned to obtain resin-dentin sticks. For μTBS and NL, specimens were tested after 24h and 1-year periods. All data were submitted to statistical analysis (α=0.05). The addition of CN provided AMA to the adhesives at all concentrations. Higher CR was observed in adhesives with higher concentration of CN. UTS, DC, WS and SO were not influenced. For μTBS an increase was observed in 0.1 and 0.5% copper group. For NL, a significant decrease was observed in all groups in comparison with control group. After 1-year, no significant reductions of μTBS and no significant increases of NL were observed for copper containing adhesives compared to the control group. The addition of CN in concentrations up to 1wt% in the 2-ER adhesive may be an alternative to provide AMA and preserve the bonding to dentin, without reducing adhesives' mechanical properties evaluated. Copyright © 2016 The Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

  5. Influence of Blood Contamination During Multimode Adhesive ...

    African Journals Online (AJOL)

    2018-01-30

    Jan 30, 2018 ... (μTBS) of multimode adhesives to dentin when using the self‑etch approach. Materials and Methods: ... adhesion, the collagen fibers collapse during the. Introduction ..... The failure mode was determined using an optical.

  6. Carbodiimide Inactivation of MMPs and Effect on Dentin Bonding

    Science.gov (United States)

    Mazzoni, A.; Apolonio, F.M.; Saboia, V.P.A.; Santi, S.; Angeloni, V.; Checchi, V.; Curci, R.; Di Lenarda, R.; Tay, F.R.; Pashley, D.H.; Breschi, L.

    2014-01-01

    The use of protein cross-linking agents during bonding procedures has been recently proposed to improve bond durability. This study aimed to use zymography and in situ zymography techniques to evaluate the ability of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) cross-linker to inhibit matrix metalloproteinase (MMP) activity. The hypotheses tested were that: (1) bonding procedures increase dentin gelatinolytic activity and (2) EDC pre-treatment prevents this enzymatic activity. The zymographic assay was performed on protein extracts obtained from dentin powder treated with Optibond FL or Scotchbond 1XT with or without 0.3M EDC pre-treatment. For in situ zymography, adhesive/dentin interfaces were created with the same adhesives applied to acid-etched dentin slabs pre-treated or not with EDC conditioner. Zymograms revealed increased expression of dentin endogenous MMP-2 and -9 after adhesive application, while the use of EDC as a primer inactivated dentin gelatinases. Results of in situ zymograpy showed that hybrid layers of tested adhesives exhibited intense collagenolytic activity, while almost no fluorescence signal was detected when specimens were pre-treated with EDC. The correlative analysis used in this study demonstrated that EDC could contribute to inactivate endogenous dentin MMPs within the hybrid layer created by etch-and-rinse adhesives. PMID:24334409

  7. In vitro evaluation of marginal microleakage in class V restorations with composite resin in bovine teeth. Laser irradiation influences and the adhesive system in the dentin pre-treatment; Avaliacao in vitro da microinfiltracao marginal em restauracoes de classe V com resina composta em dentes bovinos. Influencia da irradiacao laser e sistema adesivo no pre-tratamento dentinario

    Energy Technology Data Exchange (ETDEWEB)

    Carvalho, Wendell Lima de

    2003-07-01

    Microleakage is one of the most important reasons to restorations failure, it is the responsible for marginal colors changing, new caries, hipersensibility and pulpar diseases. Several techniques and materials have been studied to eliminate or, at least, to decrease microleakage. The cavities preparation with Er:YAG laser and autoconditioning adhesive are some of these techniques and materials. This research has the objective to compare, in vitro, microleakage in class V cavities, prepared with high rotation (conventional treatment), Er:YAG laser (Enamel-400 mj/2 Hz/128,38 J/Cm{sup 2}, Dentin 250 mJ/ 2 Hz/ 80,24 J/Cm{sup 2}) and the treatment made at dentin with autoconditioning adhesive (Clerafil SE Bond) using Er:YAG laser (with water or not water) or not using Er:YAG laser. It was used 48 bovines teeth with cavities prepared in vestibular face and gingival wall on cement enamel junction and oclusal wall on enamel. The materials used were autoconditioning adhesive (Clerafil SE Bond) and composite resin Z250. Teeth were divided into four groups of twelve samples each one, according to dentin treatment. Group 1 - Conventional cavity and autoconditioning adhesive. Group 2- Cavity prepared with Er: YAG laser and autoconditioning adhesive. Group 3 - Cavity prepared with Er:YAG laser and dentin conditioning with Er:YAG laser associated to water and autoconditioning adhesive. Group 4 - Cavity prepared with Er:YAG laser and dentin conditioning with Er: YAG laser without water and associated to autoconditioning adhesive. Teeth were restored and stocked at 37 deg C, thermocycled and placed into a 50% silver nitrate solution. Right after, teeth were sliced and evaluated on a stereo microscopic magnifying glass in order to see microleakage degree trying to follow a score from 0 to 3. The findings were submitted to Fisher, Anderson-Darling tests and to the not parametric Sen and Puri test. The results indicated that in gingival edge, the Group 2 showed less microleakage than

  8. Percolation Model of Adhesion at Polymer Interfaces

    Science.gov (United States)

    Wool, Richard P.

    1998-03-01

    Adhesion at polymer interfaces is treated as a percolation problem, where an areal density of chains Σ, of length L, contribute a number of entanglements to the interface of thickness X. The fracture energy G, is determined by the fraction of entanglements P, fractured or disentangled in the deformation zone preceding the crack tip, via G ~ P-P_c, where Pc is the percolation threshold, given by Pc = 1- M_e/Mc . For incompatible A/B interfaces reinforced with Σ diblocks or random A-B copolymers of effective length L'(L' ~ 0 for brushes and strongly adsorbed chains), we obtain P ~ ΣL/X, Pc ~ Σ _cL/X, such that G = K(Σ - Σ _c)+ G_o, where K and Go ~ 1 J/m^2 are constants. Note that Log G vs Log Σ will have an apparent slope of about 2, incorrectly suggesting that G ~ Σ ^2. For cohesive fracture, disentanglement dominates at M M*, G = G*[1-M_c/M]. For fatigue crack propagation da/dN, at welding interfaces, we obtain da/dN ~ M-5/2(t/Tr)-5/4, where t is the welding time and Tr is the reptation time. For polymer-solid interfaces, G ~ (X/R)^2. where X is the conformational width of the first layer of chains of random coil size R. The fractal nature of the percolation process is relevant to the fracture mechanism and fractography.

  9. Functional remineralization of carious dentin

    Science.gov (United States)

    Pugach, Megan Kardon

    A primary goal of dental tissue engineering is the biological reconstruction of tooth substrate destroyed by caries or other diseases affecting tooth mineralization. Traditionally, dentists treat caries by using invasive techniques to remove the diseased dental tissue and restore the lesion, ideally preventing further progression of decay. Success in strategies associated with remineralization of enamel and root caries have contributed to the less invasive prospect of remineralization of dentinal carious lesions. The central hypothesis of this dissertation is that carious dentin lesions can be remineralized if the lesions contain residual mineral. Caries Detector (CD) stained zones (pink, light pink, transparent and normal) of arrested carious dentin lesions were characterized according to microstructure by atomic force microscopy (AFM) imaging, mineral content by digital transverse microradiography, and nanomechanical properties by AFM-based nanoindentation. CD-stained and unstained zones had significantly different microstructure, mineral content and nanomechanical properties. Furthermore, the most demineralized carious zone contained residual mineral. To obtain reproducible, standardized dentin caries lesions, we characterized the lesions from an artificial carious dentin lesion model using a 0.05M acetate demineralization buffer. The artificial caries-like lesions produced by the buffer had similar mineral content and nanomechanical properties in the stained and unstained zones as natural dentin lesions. Both natural and artificial lesions had significant correlations between mineral content and nanomechanical properties. Mineral crystallite size and shape was examined by small angle x-ray scattering. Both natural and artificial carious dentin had different mineral sizes than normal dentin. Collagen in natural and artificial carious dentin lesions was examined by trichrome stain, AFM high-resolution imaging, and UV resonance Raman spectroscopy, to determine if

  10. Mechanisms of Probe Tack Adhesion of Model Acrylic Elastomers

    Science.gov (United States)

    Lakrout, Hamed; Creton, Costantino; Ahn, Dongchan; Shull, Kenneth R.

    1997-03-01

    The adhesion mechanisms of model acrylate homopolymers and copolymers are studied with an instrumented probe tack test. A video camera positioned under the transparent glass substrate records the bonding and debonding process while the force displacement curve is acquired. This setup allows to couple the observation of the cavitation and fibrillation mechanisms, occurring during the debonding of the film from the stainless steel probe, with the mechanical measurement of stress and strain. The transitions between different debonding mechanisms are critically dicussed in terms of the bulk and surface properties of the adhesive and its molecular structure.

  11. Avaliação da interação entre resina composta e diferentes adesivos dentinários Evaluation of the interaction between composite resin and different dentin adhesives

    Directory of Open Access Journals (Sweden)

    Luciana Lourenço RIBEIRO

    1999-01-01

    Full Text Available O objetivo deste estudo foi avaliar a resistência à tração de quatro diferentes sistemas adesivos. Scotchbond Multi Purpose Plus, 3M (Grupo 1, Prime & Bond 2.0, Dentsply (Grupo 2 ProBOND, Dentsply (Grupo 3, PAAMA 2, (Grupo 4 foram usados com a resina composta Glacier (SDI. Um grupo sem a utilização de qualquer sistema adesivo serviu como controle (Grupo 5. Cinqüenta espécimes foram divididos em cinco grupos com dez espécimes cada. Uma matriz de aço inoxidável com 6,0 mm de diâmetro e 1,0 mm de profundidade foi usada para se obterem dois discos de resina composta. A resina composta foi inserida em uma metade da matriz em pequenas porções e fotopolimerizada por 40 segundos. Os adesivos foram então aplicados na superfície dos discos de resina, seguindo a instrução dos fabricantes. A segunda parte da matriz foi colocada em posição e preenchida com a resina composta. Após uma hora, a matriz foi adaptada em um dispositivo especial na máquina de ensaios Kratos para determinar a resistência de união, a uma velocidade de 0,05 mm/min. Os resultados, expressos em kgf, foram: Grupo 1 (3,99 ± 1,47, Grupo 2 (4,24 ± 2,00, Grupo 3 (3,84 ± 0,88, Grupo 4 (4,33 ± 1,23 e Grupo 5 (4,21 ± 1,38. Os resultados foram analisados pelo teste estatístico ANOVA a um critério. Não houve diferença estatisticamente significante (p The purpose of this study was to evaluate the tensile bond strength of four different adhesive systems. Scotchbond Multi-Purpose Plus, 3M (Group 1, Prime & Bond 2.0, Dentsply (Group 2, ProBOND, Dentsply (Group 3, PAAMA 2, SDI (Group 4 were used with GLACIER (SDI composite resin. One group without any adhesive was used as control (Group 5. Fifty specimens were divided into 5 groups of 10 each. A stainless steel split matrix with 6.00 mm diameter and 1.00 mm depth was used to obtain two discs of composite resin. The composite resin was applied into one half of the matrix in small portions and light cured for 40 seconds

  12. Piecewise-Constant-Model-Based Interior Tomography Applied to Dentin Tubules

    Directory of Open Access Journals (Sweden)

    Peng He

    2013-01-01

    Full Text Available Dentin is a hierarchically structured biomineralized composite material, and dentin’s tubules are difficult to study in situ. Nano-CT provides the requisite resolution, but the field of view typically contains only a few tubules. Using a plate-like specimen allows reconstruction of a volume containing specific tubules from a number of truncated projections typically collected over an angular range of about 140°, which is practically accessible. Classical computed tomography (CT theory cannot exactly reconstruct an object only from truncated projections, needless to say a limited angular range. Recently, interior tomography was developed to reconstruct a region-of-interest (ROI from truncated data in a theoretically exact fashion via the total variation (TV minimization under the condition that the ROI is piecewise constant. In this paper, we employ a TV minimization interior tomography algorithm to reconstruct interior microstructures in dentin from truncated projections over a limited angular range. Compared to the filtered backprojection (FBP reconstruction, our reconstruction method reduces noise and suppresses artifacts. Volume rendering confirms the merits of our method in terms of preserving the interior microstructure of the dentin specimen.

  13. Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice.

    Science.gov (United States)

    Song, Minju; Kim, Sol; Kim, Terresa; Park, Sil; Shin, Ki-Hyuk; Kang, Mo; Park, No-Hee; Kim, Reuben

    2017-01-12

    Dental pulp is a vital organ of a tooth fully protected by enamel and dentin. When the pulp is exposed due to cariogenic or iatrogenic injuries, it is often capped with biocompatible materials in order to expedite pulpal wound healing. The ultimate goal is to regenerate reparative dentin, a physical barrier that functions as a "biological seal" and protects the underlying pulp tissue. Although this direct pulp-capping procedure has long been used in dentistry, the underlying molecular mechanism of pulpal wound healing and reparative dentin formation is still poorly understood. To induce reparative dentin, pulp capping has been performed experimentally in large animals, but less so in mice, presumably due to their small sizes and the ensuing technical difficulties. Here, we present a detailed, step-by-step method of performing a pulp-capping procedure in mice, including the preparation of a Class-I-like cavity, the placement of pulp-capping materials, and the restoration procedure using dental composite. Our pulp-capping mouse model will be instrumental in investigating the fundamental molecular mechanisms of pulpal wound healing in the context of reparative dentin in vivo by enabling the use of transgenic or knockout mice that are widely available in the research community.

  14. Penetration of amalgam constituents into dentine

    NARCIS (Netherlands)

    Scholtanus, Johannes D.; Ozcan, Mutlu; Huysmans, Marie-Charlotte D. N. J. M.

    Objectives: Amalgam restorations are replaced by adhesively placed composite resin restorations at an increasing rate. After the removal of amalgam dentine often shows marked dark discoloration that is attributed to the penetration of corrosion products from overlying amalgams. it is questioned

  15. Penetration of amalgam constituents into dentine.

    NARCIS (Netherlands)

    Scholtanus, J.D.; Ozcan, M.; Huysmans, M.C.D.N.J.M.

    2009-01-01

    OBJECTIVES: Amalgam restorations are replaced by adhesively placed composite resin restorations at an increasing rate. After the removal of amalgam dentine often shows marked dark discoloration that is attributed to the penetration of corrosion products from overlying amalgams. It is questioned

  16. New perspectives about molecular arrangement of primary and permanent dentin

    Energy Technology Data Exchange (ETDEWEB)

    Sanches Borges, Ana Flavia [Department of Dental Materials at Piracicaba Dental School, State University of Campinas, Piracicaba, SP (Brazil); Andrade Bitar, Renata [Biomedical Engineering, Valley of Paraiba University (UNIVAP) (Brazil); Kantovitz, Kamila Rosamilia [Department of Pediatric Dentistry at Piracicaba Dental School, State University of Campinas, Piracicaba, SP (Brazil); Bortollazo Correr, Americo [Department of Dental Materials at Piracicaba Dental School, State University of Campinas, Piracicaba, SP (Brazil); Martin, Airton Abrahao [Biomedical Engineering Post Graduation, Valley of Paraiba University (UNIVAP) (Brazil); Puppin-Rontani, Regina Maria [Department of Pediatric Dentistry at Piracicaba Dental School, State University of Campinas, Piracicaba, SP (Brazil)], E-mail: rmpuppin@fop.unicamp.br

    2007-12-30

    The dentin quality of primary and permanent teeth was inspected by Fourier transformed Raman spectroscopy (FT-Raman); scanning electron microscopy/energy-dispersive spectroscopy (SEM/EDS) and hardness test. Middle dentin of crowns were reached by carbide bur abrading providing a uniform smear layer. Phosphoric acid was applied in order to simulate the etching of total etching adhesive systems. The groups were (n = 10): G1 (primary dentin smear layer); G2 (35% phosphoric acid etched primary dentin); G3 (permanent dentin smear layer); G4 (35% phosphoric acid etched permanent dentin). FT-Raman results were subjected to cluster analysis. SEM/EDS were made in order to add the data obtained by FT-Raman. The hardness data were subjected to ANOVA and Tukey test. FT-Raman showed differences among groups, either to organic or inorganic content. For the organic content, primary and permanent dentin became similar after the etching; conversely, the inorganic content showed differences for the two substrates. Hardness test showed no significant differences between primary and permanent dentin, before or after etching, but the etching decreased these values. The mineral content arrangement of primary dentin is different from permanent dentin, independently of the etching. The substrate type did no influence the hardness, but the etching decreased it.

  17. Effect of sonic application mode on the resin-dentin bond strength and dentin permeability of self-etching systems.

    Science.gov (United States)

    Mena-Serrano, Alexandra; Costa, Thays Regina Ferreira da; Patzlaff, Rafael Tiago; Loguercio, Alessandro Dourado; Reis, Alessandra

    2014-10-01

    To compare manual and sonic adhesive application modes in terms of the permeability and microtensile bond strength of a self-etching adhesive applied in the one-step or two-step protocol. Self-etching All Bond SE (Bisco) was applied as a one- or a two-step adhesive under manual or sonic vibration modes on flat occlusal dentin surfaces of 64 human molars. Half of the teeth were used to measure the hydraulic conductance of dentin at 200 cm H₂O hydrostatic pressure for 5 min immediately after the adhesive application. In the other half, composite buildups (Opallis) were constructed incrementally to create resin-dentin sticks with a cross-sectional area of 0.8 mm² to be tested in tension (0.5 mm/min) immediately after restoration placement. Data were analyzed using a two-way ANOVA and Tukey's test (α = 0.05). The fluid conductance of dentin was significantly reduced by the sonic vibration mode for both adhesives, but no effect on the bond strength values was observed for either adhesive. The sonic application mode at an oscillating frequency of 170 Hz can reduce the fluid conductance of the one- and two-step All Bond SE adhesive when applied on dentin.

  18. Advances in modeling and design of adhesively bonded systems

    CERN Document Server

    Kumar, S

    2013-01-01

    The book comprehensively charts a way for industry to employ adhesively bonded joints to make systems more efficient and cost-effective Adhesively bonded systems have found applications in a wide spectrum of industries (e.g., aerospace, electronics, construction, ship building, biomedical, etc.) for a variety of purposes. Emerging adhesive materials with improved mechanical properties have allowed adhesion strength approaching that of the bonded materials themselves. Due to advances in adhesive materials and the many potential merits that adhesive bonding offers, adhesive bonding has replac

  19. A mechanical model of biomimetic adhesive pads with tilted and hierarchical structures.

    Science.gov (United States)

    Schargott, M

    2009-06-01

    A 3D model for hierarchical biomimetic adhesive pads is constructed. It is based on the main principles of the adhesive pads of the Tokay gecko and consists of hierarchical layers of vertical or tilted beams, where each layer is constructed in such a way that no cohesion between adjacent beams can occur. The elastic and adhesive properties are calculated analytically and numerically. For the adhesive contact on stochastically rough surfaces, the maximum adhesion force increases with increasing number of hierarchical layers. Additional calculations show that the adhesion force also depends on the height spectrum of the rough surface.

  20. A mechanical model of biomimetic adhesive pads with tilted and hierarchical structures

    Energy Technology Data Exchange (ETDEWEB)

    Schargott, M [Institute of Mechanics, Technische Universitaet Berlin, Strd 17 Juni 135, 10623 Berlin (Germany)], E-mail: martin.schargott@tu-berlin.de

    2009-06-01

    A 3D model for hierarchical biomimetic adhesive pads is constructed. It is based on the main principles of the adhesive pads of the Tokay gecko and consists of hierarchical layers of vertical or tilted beams, where each layer is constructed in such a way that no cohesion between adjacent beams can occur. The elastic and adhesive properties are calculated analytically and numerically. For the adhesive contact on stochastically rough surfaces, the maximum adhesion force increases with increasing number of hierarchical layers. Additional calculations show that the adhesion force also depends on the height spectrum of the rough surface.

  1. A mechanical model of biomimetic adhesive pads with tilted and hierarchical structures

    International Nuclear Information System (INIS)

    Schargott, M

    2009-01-01

    A 3D model for hierarchical biomimetic adhesive pads is constructed. It is based on the main principles of the adhesive pads of the Tokay gecko and consists of hierarchical layers of vertical or tilted beams, where each layer is constructed in such a way that no cohesion between adjacent beams can occur. The elastic and adhesive properties are calculated analytically and numerically. For the adhesive contact on stochastically rough surfaces, the maximum adhesion force increases with increasing number of hierarchical layers. Additional calculations show that the adhesion force also depends on the height spectrum of the rough surface

  2. Bond strength of compomers to dentin using acidic primers.

    Science.gov (United States)

    Tate, W H; You, C; Powers, J M

    1999-10-01

    To determine the in vitro bond strengths of seven compomer/bonding agent restorative systems to human dentin. Seven compomer/bonding agents were bonded to human dentin, stored in water at 37 degrees C for 24 hours, and debonded in tension. Bonding conditions were with and without phosphoric acid etching, with and without the use of combined primer/bonding agents, and under moist and wet bond interfaces. Without phosphoric acid etching, F2000/F2000 Compomer Primer/Adhesive and F2000/Single Bond Dental Adhesive System were less sensitive to dentin wetness. With moist dentin, bond strengths of Dyract/Prime & Bond 2.1, Dyract AP/Prime & Bond 2.1, Hytac/OSB light-curing, one-component bonding agent, F2000/Single Bond, and Freedom/STAE single component light-cured dentin/enamel adhesive system, were improved with phosphoric acid etching. Also, with moist dentin, the bond strength of F2000/F2000 Compomer Primer/Adhesive in the 3M Clicker dispensing system was higher without phosphoric acid etching, whereas bonds of Compoglass/Syntac Single-component were not affected by phosphoric acid etching. Bonding did not occur without primer/bonding agent, regardless of surface condition or use of phosphoric acid etching.

  3. Peritubular dentin lacks piezoelectricity.

    Science.gov (United States)

    Habelitz, S; Rodriguez, B J; Marshall, S J; Marshall, G W; Kalinin, S V; Gruverman, A

    2007-09-01

    Dentin is a mesenchymal tissue, and, as such, is based on a collagenous matrix that is reinforced by apatite mineral. Collagen fibrils show piezoelectricity, a phenomenon that is used by piezoresponse force microscopy (PFM) to obtain high-resolution images. We applied PFM to image human dentin with 10-nm resolution, and to test the hypothesis that zones of piezoactivity, indicating the presence of collagen fibrils, can be distinguished in dentin. Piezoelectricity was observed by PFM in the dentin intertubular matrix, while the peritubular dentin remained without response. High-resolution imaging of chemically treated intertubular dentin attributed the piezoelectric effect to individual collagen fibrils that differed in the signal strength, depending on the fibril orientation. This study supports the hypothesis that peritubular dentin is a non-collagenous tissue and is thus an exception among mineralized tissues that derive from the mesenchyme.

  4. Comparative study of etched enamel and dentin for the adhesion of composite resins with the Er:YAG 2,94 μm laser and CO2 9,6 μm laser: morphological (SEM) and tensile bond strength analysis

    International Nuclear Information System (INIS)

    Marraccini, Tarso Mugnai

    2002-01-01

    The aim of this study was to evaluate and compare the tensile bond strength of a composite resin adhered to the enamel and dentin which have received superficial irradiation with an Er:YAG laser (2.94 μm) or with CO 2 laser ( 9.6 μm) and later on etched with the phosphoric acid at 35%. After the use of the adhesive system, resin cones were made on the etched surfaces by both lasers and tensile bond strength tests were performed. All samples were observed at the SEM - there was an increase of the degree of fusion and resolidification in the irradiated enamel and dentin samples with the CO 2 laser (9.6 μm), creating a vitrified layer with tiny craters. With the Er:YAG laser (2.94 μm) there were typical morphological explosive microablation with the exposition of the tubules in the dentin.The surface acquired by the association of the CO 2 laser ( 9.6 μm) plus acid etching no longer presented the aspect of fusion being this layer completely removed. There were statistical significant differences among ali three methods of etching in the treatment of the enamel and dentin surface. The tensile bond strength test showed that etching of these enamel and dentin surfaces with acid exclusively (control group) presented great values, surpassing the values of the etching acquired with the Er:YAG laser (2.94 μ) plus acid or the CO 2 laser (9.6 μm) plus acid. With the parameters used in this experiment the Er:YAG laser (2.94 μm) showed to be more effective than the CO 2 laser (9.6 μm) for the hard dental surfaces etching procedure. (author)

  5. Tensile bond srength between composite resin using different adhesive systems Avaliação da resistência à ruptura por tração entre resina composta e diversos adesivos dentinários

    Directory of Open Access Journals (Sweden)

    Patrícia Dias

    2002-11-01

    Full Text Available The aim of this study was evaluate the tensile bond strength (TBS among nine adhesive systems and one composite resin. The groups were made as follows: Single Bond/3M (G1, Etch & Prime 3.0 /Degussa (G2, Bond 1/Jeneric/Pentron (G3, Prime & Bond 2.1/Dentsply (G4, OptiBond FL/Kerr (G5, Stae/SDI (G6, Snap Bond/ Copalite-Cooley & Cooley (G7, Prime & Bond NT/Dentsply (G8, Scotchbond Multi Purpose Plus/3M (G9. The control group (G10 was made only with the composite resin (Z100/3M. One hundred specimens were made, 10 for each group. There were significant differences on TBS among groups. G3 showed the hightest TBS in comparison to other tested groups. G10 presented higher TBS than all groups. O objetivo desta pesquisa foi investigar in vitro a resistência de união entre uma resina composta e nove sistemas adesivos dentinários. Os adesivos estudados foram assim agrupados: Single Bond/3M (G1, Etch & Prime 3.0/ Degussa (G2, Bond 1/Jeneric/Pentron (G3, Prime & Bond 2.1/Dentsply (G4, OptiBond FL/Kerr (G5, Stae/SDI (G6, Snap Bond/Copalite (G7, Prime & Bond NT/Dentsply (G 8, Scotchbond Multi Purpose Plus/3M (G9. O Grupo controle (G10. foi confeccionado somente com a resina composta (Z100/3M. Foram confeccionados 100 espécimes, 10 para cada grupo. Houve diferenças estatísticas significantes entre os grupos. O grupo 3 foi o que mostrou a mais alta resistência em comparação aos nove testados. O grupo controle (G10 apresentou a mais alta resistência entre todos os Grupos.  

  6. Morphological Analysis of Dentin Surface after Conditioning with Two Different methods: Chemical and Mechanical.

    Science.gov (United States)

    Rafael, Caroline Freitas; Quinelato, Valquíria; Morsch, Carolina Schaffer; DeDeus, Gustavo; Reis, Claudia Mendonca

    2016-01-01

    Alternative pretreatment strategies of dentin and adhesionare constantly being developed and studied with the goal of improving the adhesion of resin restorative materials with this tissue. The objectives of the present study were to evaluate the ability of airborne-particle abrasion (APA) with aluminum oxide on dentin to remove the smear layer and the effects produced on the dentin microstructure. The phosphoric acid (PA) was used for a comparison. For that, 20 human third molars were randomly allocated into two experimental groups, according to the dentin pretreatment method used: G1 (N = 10) - PA, G2 (N = 10) -APA. For dentin surface analyses, an environmental scanning electron microscope (ESEM) was employed to observe dentin surfaces before and after the procedures. Before pretreatment, the specimens of both groups were smear covered. After pretreatment, the G1 images revealed dentin tubule orifices opened, enlarged and some erosive effects. (G2) exposed tubule orifices without enlargement, but crack-like alterations were observed on the surfaces. In this way, APA with aluminum oxide was able to remove the smear layer. The influences of the dentin roughness on adhesion and the consequences on dentin integrity and hardness need further investigations. A good conditioning of the dentin before cementation is necessary in order to obtain a satisfactory rehabilitation in adhesive dentistry. So, it is necessary to know all methods to do it.

  7. Impact of combined CO2 laser irradiation and fluoride on enamel and dentin biofilm-induced mineral loss.

    Science.gov (United States)

    Esteves-Oliveira, Marcella; El-Sayed, Karim Fawzy; Dörfer, Christof; Schwendicke, Falk

    2017-05-01

    The caries-protective effects of CO 2 laser irradiation on dental enamel have been demonstrated using chemical demineralization models. We compared the effect of CO 2 laser irradiation, sodium fluoride, or both on biofilm-induced mineral loss (∆Z) and Streptococcus mutans adhesion to enamel and dentin in vitro. Ground, polished bovine enamel, and dentin samples were allocated to four groups (n = 12/group): no treatment (C); single 22,600-ppm fluoride (F) varnish (5 % NaF) application; single CO 2 laser treatment (L) with short pulses (5 μs/λ = 10.6 μm); and laser and subsequent fluoride treatment (LF). Samples were sterilized and submitted to an automated mono-species S. mutans biofilm model. Brain heart infusion plus 5 % sucrose medium was provided eight times daily, followed by rinses with artificial saliva. After 10 days, bacterial numbers in biofilms were enumerated as colony-forming units/ml (CFU/ml) (n = 7/group). ∆Z was assessed using transversal microradiography (n = 12/group). Univariate ANOVA with post hoc Tukey honestly-significant-difference test was used for statistical analysis. Bacterial numbers were significantly higher on dentin than enamel (p  0.05). In dentin, only LF (163/227) significantly reduced ∆Z (p fluoride application was required to protect dentin.

  8. Bond strength of universal adhesives: A systematic review and meta-analysis.

    Science.gov (United States)

    Rosa, Wellington Luiz de Oliveira da; Piva, Evandro; Silva, Adriana Fernandes da

    2015-07-01

    A systematic review was conducted to determine whether the etch-and-rinse or self-etching mode is the best protocol for dentin and enamel adhesion by universal adhesives. This report followed the PRISMA Statement. A total of 10 articles were included in the meta-analysis. Two reviewers performed a literature search up to October 2014 in eight databases: PubMed, Web of Science, Scopus, BBO, SciELO, LILACS, IBECS and The Cochrane Library. In vitro studies evaluating the bond strength of universal adhesives to dentin and/or enamel by the etch-and-rinse and self-etch strategies were eligible to be selected. Statistical analyses were conducted using RevMan 5.1 (The Cochrane Collaboration, Copenhagen, Denmark). A global comparison was performed with random-effects models at a significance level of puniversal adhesives (p≥0.05). However, for the ultra-mild All-Bond Universal adhesive, the etch-and-rinse strategy was significantly different than the self-etch mode in terms of dentin micro-tensile bond strength, as well as in the global analysis of enamel micro-tensile and micro-shear bond strength (p≤0.05). The enamel bond strength of universal adhesives is improved with prior phosphoric acid etching. However, this effect was not evident for dentin with the use of mild universal adhesives with the etch-and-rinse strategy. Selective enamel etching prior to the application of a mild universal adhesive is an advisable strategy for optimizing bonding. Copyright © 2015 Elsevier Ltd. All rights reserved.

  9. Dental Stem Cell Migration on Pulp Ceiling Cavities Filled with MTA, Dentin Chips, or Bio-Oss

    Directory of Open Access Journals (Sweden)

    Stefania Lymperi

    2015-01-01

    Full Text Available MTA, Bio-Oss, and dentin chips have been successfully used in endodontics. The aim of this study was to assess the adhesion and migration of dental stem cells on human pulp ceiling cavities filled with these endodontic materials in an experimental model, which mimics the clinical conditions of regenerative endodontics. Cavities were formed, by a homemade mold, on untouched third molars, filled with endodontic materials, and observed with electron microscopy. Cells were seeded on cavities’ surface and their morphology and number were analysed. The phenomenon of tropism was assessed in a migration assay. All three materials demonstrated appropriate microstructures for cell attachment. Cells grew on all reagents, but they showed a differential morphology. Moreover, variations were observed when comparing cells numbers on cavity’s filling versus the surrounding dentine disc. The highest number of cells was recorded on dentin chips whereas the opposite was true for Bio-Oss. This was confirmed in the migration assay where a statistically significant lower number of cells migrated towards Bio-Oss as compared to MTA and dentin chips. This study highlights that MTA and dentin chips have a greater potential compared to Bio-Oss regarding the attraction of dental stem cells and are good candidates for bioengineered pulp regeneration.

  10. Enamel and dentine demineralization by a combination of starch and sucrose in a biofilm – caries model

    Directory of Open Access Journals (Sweden)

    Juliana Nunes BOTELHO

    2016-01-01

    Full Text Available Abstract Sucrose is the most cariogenic dietary carbohydrate and starch is considered non-cariogenic for enamel and moderately cariogenic for dentine. However, the cariogenicity of the combination of starch and sucrose remains unclear. The aim of this study was to evaluate the effect of this combination on Streptococcus mutans biofilm composition and enamel and dentine demineralization. Biofilms of S. mutans UA159 were grown on saliva-coated enamel and dentine slabs in culture medium containing 10% saliva. They were exposed (8 times/day to one of the following treatments: 0.9% NaCl (negative control, 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose. To simulate the effect of human salivary amylase on the starch metabolization, the biofilms were pretreated with saliva before each treatment and saliva was also added to the culture medium. Acidogenicity of the biofilm was estimated by evaluating (2 times/day the culture medium pH. After 4 (dentine or 5 (enamel days of growth, biofilms (n = 9 were individually collected, and the biomass, viable microorganism count, and polysaccharide content were quantified. Dentine and enamel demineralization was assessed by determining the percentage of surface hardness loss. Biofilms exposed to starch + sucrose were more acidogenic and caused higher demineralization (p < 0.0001 on either enamel or dentine than those exposed to each carbohydrate alone. The findings suggest that starch increases the cariogenic potential of sucrose.

  11. Enamel and dentin bond strength following gaseous ozone application.

    Science.gov (United States)

    Cadenaro, Milena; Delise, Chiara; Antoniollo, Francesca; Navarra, Ottavia Chiara; Di Lenarda, Roberto; Breschi, Lorenzo

    2009-08-01

    To evaluate the effects of gaseous ozone application on enamel and dentin bond strength produced by two self-etching adhesive systems. The shear bond strength test was conducted to assess adhesion on enamel (protocol 1), while the microtensile bond strength test was performed on dentin (protocol 2). Protocol 1: 96 bovine incisors were randomly divided into 4 groups, and enamel surfaces were bonded in accordance with the following treatments: (1E) ozone + Clearfil Protect Bond; (2E) Clearfil Protect Bond (control); (3E) ozone + Xeno III; (4E) Xeno III (control). Ozone gas was applied for 80 s. Shear bond strength was measured with a universal testing machine. Protocol 2: 40 noncarious human molars were selected. Middle/deep dentin was exposed and bonded in accordance with the following treatments: (1D) ozone+Clearfil Protect Bond; (2D) Clearfil Protect Bond (control); (3D) ozone+Xeno III (4D) Xeno III (control). Four-mm-thick buildups were built on the adhesives, then specimens were sectioned in accordance with the nontrimming technique. Specimens were stressed until failure occurred, and failure modes were analyzed. Shear bond and microtensile bond strength data were analyzed using two-way ANOVA and Tukey's post-hoc test. No statistical differences were found between ozone treated specimens and controls, neither on enamel nor on dentin irrespective of the tested adhesive. Clearfil Protect Bond showed higher bond strength to enamel than Xeno III, irrespective of the ozone treatment (p enamel and dentin bond strength.

  12. Concomitant caries and calculus formation from in situ dentin caries model [v1; ref status: indexed, http://f1000r.es/VnGedU

    Directory of Open Access Journals (Sweden)

    Frederico B de Sousa

    2013-01-01

    Full Text Available The aim of this study was to test the possibility of the concomitant formation of calculus deposits and caries from in situ dentin caries model for short time periods. Six volunteers wore palatal removal appliances with four polished dentin specimens protected from intra-oral mechanical forces for up to 14 days. Each volunteer applied a 50% sucrose solution (four times a day on the specimens and performed a daily mouthwash with 0.05% NaF. Samples were removed after 2, 5, 9 and 14 days in situ. Demineralization was analyzed by stereomicroscopy and SEM (secondary electrons and backscattered electrons modes and calculus was analyzed by energy dispersive spectroscopy and fluorescence spectroscopy. Seventeen samples, at least one sample from each volunteer, presented dental calculus on both carious and non-carious ones, detected in all time intervals. Ca/P ratios of dental calculus ranged from 1.1 to 1.7. Some large calculus deposits on carious surfaces were confirmed by fluorescence. In conclusion, concomitant caries and calculus formation can be found in dentin caries formed in situ. This has important repercussions on the study of surface phenomena on the interface between hard dental tissues and dental plaque.

  13. Optimized Baxter model of protein solutions : Electrostatics versus adhesion

    NARCIS (Netherlands)

    Prinsen, P.; Odijk, T.

    2004-01-01

    A theory is set up of spherical proteins interacting by screened electrostatics and constant adhesion, in which the effective adhesion parameter is optimized by a variational principle for the free energy. An analytical approach to the second virial coefficient is first outlined by balancing the

  14. A rat hysteropexy model for evaluating adhesion formation and comparison of two different structured meshes.

    Science.gov (United States)

    Gokmen-Karasu, Ayse Filiz; Aydin, Serdar; Sonmez, Fatma Cavide; Adanir, Ilknur; Ilhan, Gulsah; Ates, Seda

    2017-11-01

    Peritonization of mesh during sacrohysteropexy is generally advocated to prevent adhesions to the viscera; however, randomized clinical trials are lacking, and peritonization may not be completely possible in a laparoscopic hysteropexy procedure. Our main objective was to describe a basic experimental rat sacrohysteropexy model. We hypothesized that even when peritoneal closure was omitted, using composite mesh would result in less adhesions to the viscera. Twenty in-bred female virgin Wistar Hannover rats were used in this study. Standardized hysteropexy procedure and adhesion model is described step by step with two different mesh materials: polypropylene and a composite polyester. Mesh was anchored between the posterior cervix and anterior longitudinal ligament of the lumbar vertebrae. Macroscopic adhesion scores and histopathological tissue reaction was investigated. Macroscopically, the surface area involved in adhesions was similar between groups. However, adhesions in the polypropylene group were more dense, required sharp dissection for lysis, and yielded higher total macroscopic adhesion scores (p < 0.001). Histologically, a more pronounced host inflammatory response was encountered in the polyester group (p < 0.001). We describe a rat hysteropexy model and a previously established uterine adhesion model. Adhesion scores in the composite mesh group were lower, and bowel involvement was not seen. Our findings are promising, and further research investigating antiadhesive composite mesh use for hysterosacropexy would be appropriate, especially when peritoneal closure is omitted.

  15. An innovative wheel–rail contact model for railway vehicles under degraded adhesion conditions

    International Nuclear Information System (INIS)

    Meli, E.; Ridolfi, A.

    2015-01-01

    The accurate modelling of the wheel–rail contact plays a fundamental role in the railway field since the contact forces heavily affect the vehicle dynamics, the wear of the contact surfaces and the vehicle safety. Concerning the wheel–rail contact, an important open problem is represented by the degraded adhesion. A realistic adhesion model is quite difficult to obtain because of the complex and highly non-linear behaviour of the adhesion coefficient and the presence of external unknown contaminants (the third body); this is especially true when degraded adhesion and large sliding between the wheel and rail contact surfaces occur.In this work the authors present an adhesion model particularly developed to describe degraded adhesion conditions. The new approach will have to be suitable to be employed within the wheel–rail contact models typical of the multibody applications. In other words, the contact model, comprising the new adhesion model, will have to guarantee a good accuracy and, at the same time, a high numerical efficiency to be implemented directly online inside the general multibody model of the vehicles (e.g. in Matlab-Simulink or Simpack environments) ( www.mathworks.com http://www.mathworks.com , 2012; www.simpack.com http://www.simpack.com , 2012).The model analysed in the paper is based on some of the main phenomena characterising the degraded adhesion, such as large sliding at the contact interface, high energy dissipation, the consequent cleaning effect on the contact surfaces and the final adhesion recovery due to the removal of external unknown contaminants.The adhesion model has been validated because of the experimental data provided by Trenitalia S.p.A. coming from on-track tests performed in Velim (Czech Republic). The tests have been carried out on a straight railway track under degraded adhesion conditions with the railway vehicle UIC-Z1 equipped with a fully-working Wheel Slide Protection (WSP) system.The validation highlighted the

  16. An innovative wheel–rail contact model for railway vehicles under degraded adhesion conditions

    Energy Technology Data Exchange (ETDEWEB)

    Meli, E., E-mail: enrico.meli@unifi.it; Ridolfi, A., E-mail: a.ridolfi@unifi.it [University of Florence, Department of Industrial Engineering (Italy)

    2015-03-15

    The accurate modelling of the wheel–rail contact plays a fundamental role in the railway field since the contact forces heavily affect the vehicle dynamics, the wear of the contact surfaces and the vehicle safety. Concerning the wheel–rail contact, an important open problem is represented by the degraded adhesion. A realistic adhesion model is quite difficult to obtain because of the complex and highly non-linear behaviour of the adhesion coefficient and the presence of external unknown contaminants (the third body); this is especially true when degraded adhesion and large sliding between the wheel and rail contact surfaces occur.In this work the authors present an adhesion model particularly developed to describe degraded adhesion conditions. The new approach will have to be suitable to be employed within the wheel–rail contact models typical of the multibody applications. In other words, the contact model, comprising the new adhesion model, will have to guarantee a good accuracy and, at the same time, a high numerical efficiency to be implemented directly online inside the general multibody model of the vehicles (e.g. in Matlab-Simulink or Simpack environments) ( www.mathworks.com http://www.mathworks.com , 2012; www.simpack.com http://www.simpack.com , 2012).The model analysed in the paper is based on some of the main phenomena characterising the degraded adhesion, such as large sliding at the contact interface, high energy dissipation, the consequent cleaning effect on the contact surfaces and the final adhesion recovery due to the removal of external unknown contaminants.The adhesion model has been validated because of the experimental data provided by Trenitalia S.p.A. coming from on-track tests performed in Velim (Czech Republic). The tests have been carried out on a straight railway track under degraded adhesion conditions with the railway vehicle UIC-Z1 equipped with a fully-working Wheel Slide Protection (WSP) system.The validation highlighted the

  17. Effect of desensitizing agents on dentin permeability.

    Science.gov (United States)

    Ishihata, Hiroshi; Kanehira, Masafumi; Nagai, Tomoko; Finger, Werner J; Shimauchi, Hidetoshi; Komatsu, Masashi

    2009-06-01

    To investigate the in vitro efficacy of two dentin desensitizing products at reducing liquid permeability through human dentin discs. The tested hypothesis was that the products, in spite of different chemical mechanisms were not different at reducing or eliminating flow through dentin discs. Dentin slices (1 mm thick) were prepared from 16 extracted human third molars and their permeability was indirectly recorded in a split chamber model, using a chemiluminescence technique, after EDTA treatment (control), after soaking with albumin, and after desensitizer application. Two products were studied: MS Coat, a self-curing resin-containing oxalate product, and Gluma Desensitizer, a glutaraldehyde/HEMA-based agent without initiator. The dentin slices were mounted between an upper chamber, filled with an aqueous solution of 1% potassium ferricyanide and 0.3% hydrogen peroxide, and a lower chamber filled with 1% sodium hydroxide solution and 0.02% luminol. The upper solution was pressurized, and upon contact with the luminol solution a photochemical signal was generated and recorded as a measure of permeability throughout two consecutive pressurizing cycles at 2.5 and 13 kPa (26 and 133 cm H2O), respectively. The permeability of the control and albumin-soaked samples was similarly high. After application of the desensitizing agents, dentin permeability was reduced to virtually zero at both pressure levels (P < 0.001).

  18. Modification of dentin surface to enamel-like structure: A potential strategy for improving dentin bonding durability, desensitizing and self-repairing

    Directory of Open Access Journals (Sweden)

    Hongye Yang

    2014-01-01

    Full Text Available Introduction: Current theories of dentin bonding are based on the concept of "hybrid layer". However, the histological complexity of dentin, as well as the vulnerability of the hybrid layer, goes against the long-term effect of dentin bonding. At the same time, post-operative sensitivity is more likely to occur after traditional adhesive restoration. The Hypothesis: Compared to dentin bonding, enamel bonding exhibits a more optimal immediate and long-term performance, owing to its higher degree of mineralization, well-arranged enamel crystals and the porous structure after etching. Moreover, "enamel hypersensitivity" is never going to happen due to the lack of tubules existing in dentin. In light of this phenomenon, we brought up the concept and the proposal method to form an "enamel-like" dentin, simulating enamel structure to achieve satisfying durability of dentin bonding and obtain good performance for preventing post-operative sensitivity. With the application of mesoporous silicon bi-directionally binding to hydroxyapatite of dentin itself and hydroxyapatite nanorods synthetized in vitro, we may be able to form an enamel-like "functional layer" via ion-regulating self-assembly. Evaluation of Hypothesis: This paper explains how to achieve dentin enamel-like modification by chemical methods, especially, details the strategies and possible mechanisms of the hypothesis. Validation of the hypothesis is more likely to eliminate the adverse effect of dentinal fluid, improve long-term performance of dentin bonding, offer strategies for desensitizing treatment and self-repairing carious-affected dentin, and furthermore, provide the possibility to introduce new theories of dentin bonding.

  19. Creep simulation of adhesively bonded joints using modified generalized time hardening model

    Energy Technology Data Exchange (ETDEWEB)

    Sadigh, Mohammad Ali Saeimi [Azarbaijan Shahid Madani University, Tabriz (Iran, Islamic Republic of)

    2016-04-15

    Creep behavior of double lap adhesively bonded joints was investigated using experimental tests and numerical analysis. Firstly, uniaxial creep tests were carried out to obtain the creep characteristics and constitutive parameters of the adhesive at different stress and temperature levels. Generalized time hardening model was used to predict the creep behavior of the adhesive. This model was modified to simulate the creep behavior at different stress and temperature levels. Secondly, the developed model was used to simulate the creep behavior of bonded joints using finite element based numerical analysis. Creep deformations of the joints were measured experimentally and good agreement was observed in comparison with the results obtained using numerical simulation. Afterward, stress redistribution due to the creep along the adhesively bonded joint was obtained numerically. It was observed that temperature level had a significant effect on the stress redistribution along the adhesive thickness.

  20. Influence of voids in the hybrid layer based on self-etching adhesive systems: a 3-D FE analysis

    Directory of Open Access Journals (Sweden)

    Ana Paula Martini

    2009-01-01

    Full Text Available The presence of porosities at the dentin/adhesive interface has been observed with the use of new generation dentin bonding systems. These porosities tend to contradict the concept that etching and hybridization processes occur equally and simultaneously. Therefore, the aim of this study was to evaluate the micromechanical behavior of the hybrid layer (HL with voids based on a self-etching adhesive system using 3-D finite element (FE analysis. MATERIAL AND METHODS: Three FE models (Mr were built: Mr, dentin specimen (41x41x82 μm with a regular and perfect (i.e. pore-free HL based on a self-etching adhesive system, restored with composite resin; Mp, similar to M, but containing 25% (v/v voids in the HL; Mpp, similar to Mr, but containing 50% (v/v voids in the HL. A tensile load (0.03N was applied on top of the composite resin. The stress field was obtained by using Ansys Workbench 10.0. The nodes of the base of the specimen were constrained in the x, y and z axes. The maximum principal stress (σmax was obtained for all structures at the dentin/adhesive interface. RESULTS: The Mpp showed the highest peak of σmax in the HL (32.2 MPa, followed by Mp (30 MPa and Mr (28.4 MPa. The stress concentration in the peritubular dentin was high in all models (120 MPa. All other structures positioned far from voids showed similar increase of stress. CONCLUSION: Voids incorporated into the HL raised the σmax in this region by 13.5%. This behavior might be responsible for lower bond strengths of self-etching and single-bottle adhesives, as reported in the literature.

  1. Modeling cell adhesion and proliferation: a cellular-automata based approach.

    Science.gov (United States)

    Vivas, J; Garzón-Alvarado, D; Cerrolaza, M

    Cell adhesion is a process that involves the interaction between the cell membrane and another surface, either a cell or a substrate. Unlike experimental tests, computer models can simulate processes and study the result of experiments in a shorter time and lower costs. One of the tools used to simulate biological processes is the cellular automata, which is a dynamic system that is discrete both in space and time. This work describes a computer model based on cellular automata for the adhesion process and cell proliferation to predict the behavior of a cell population in suspension and adhered to a substrate. The values of the simulated system were obtained through experimental tests on fibroblast monolayer cultures. The results allow us to estimate the cells settling time in culture as well as the adhesion and proliferation time. The change in the cells morphology as the adhesion over the contact surface progress was also observed. The formation of the initial link between cell and the substrate of the adhesion was observed after 100 min where the cell on the substrate retains its spherical morphology during the simulation. The cellular automata model developed is, however, a simplified representation of the steps in the adhesion process and the subsequent proliferation. A combined framework of experimental and computational simulation based on cellular automata was proposed to represent the fibroblast adhesion on substrates and changes in a macro-scale observed in the cell during the adhesion process. The approach showed to be simple and efficient.

  2. Amalgam stained dentin: a proper substrate for bonding resin composite?

    NARCIS (Netherlands)

    Scholtanus, J.D.

    2016-01-01

    Nowadays the use of dental amalgam is mostly abandoned and substituted by tooth colored resin composites that can be bonded to teeth tissues by adhesive techniques. The aim of this thesis was to find out whether dark stained dentin, as often observed after removal of amalgam restorations and

  3. Evaluation of polyethylene glycol/polylactic acid films in the prevention of adhesions in the rabbit adhesion formation and reformation sidewall models.

    Science.gov (United States)

    Rodgers, K; Cohn, D; Hotovely, A; Pines, E; Diamond, M P; diZerega, G

    1998-03-01

    To assess the efficacy of bioresorbable films consisting of various polyethylene glycol 6000 and polylactic acid block copolymers on the formation and reformation of adhesions in rabbit models of adhesion development between the sidewall to the adjacent cecum and bowel. The composition of the different polymers was expressed by the number of monomeric units in the block, namely, ethylene oxide (EO) and lactic acid (LA), respectively. Studies of the efficacy of EO/LA films were conducted in rabbit sidewall adhesion formation studies in the presence and absence of blood and in rabbit adhesion reformation studies. REPEL (Life Medical Sciences, Edison, NJ), a film of EO/LA ratio 3.0 manufactured under commercial conditions, was also tested in these animal models. University-based laboratory. New Zealand white rabbits. Placement of films of various EO/LA ratios at the site of injury to the parietal peritoneum. Adhesion formation and reformation. Films of various EO/LA ratios, Seprafilm (Genzyme, Cambridge, MA) and Interceed (Johnson and Johnson Medical, Arlington, TX) placed over an area of excised sidewall at the time of initial injury were highly efficacious in the prevention of adhesion formation. A film of EO/LA ratio 3.7, in contrast with Interceed, was also shown to maintain maximal efficacy in the reduction of adhesion formation in the presence of blood. Further, a film of EO/LA ratio 3.0 produced under commercial conditions, REPEL, was highly efficacious in reducing adhesion development in the rabbit models of adhesion and reformation. These studies suggest that bioresorbable EO/LA films reduced adhesion development in rabbit models of adhesion formation and reformation.

  4. Current dental adhesives systems. A narrative review.

    Science.gov (United States)

    Milia, Egle; Cumbo, Enzo; Cardoso, Rielson Jose A; Gallina, Giuseppe

    2012-01-01

    Adhesive dentistry is based on the development of materials which establish an effective bond with the tooth tissues. In this context, adhesive systems have attracted considerable research interest in recent years. Successful adhesive bonding depends on the chemistry of the adhesive, on appropriate clinical handling of the material as well as on the knowledge of the morphological changes caused on dental tissue by different bonding procedures. This paper outlines the status of contemporary adhesive systems, with particular emphasis on chemical characteristics and mode of interaction of the adhesives with enamel and dentinal tissues. Dental adhesives are used for several clinical applications and they can be classified based on the clinical regimen in "etch-and-rinse adhesives" and "self-etch adhesives". Other important considerations concern the different anatomical characteristics of enamel and dentine which are involved in the bonding procedures that have also implications for the technique used as well as for the quality of the bond. Etch-and-rinse adhesive systems generally perform better on enamel than self-etching systems which may be more suitable for bonding to dentine. In order to avoid a possible loss of the restoration, secondary caries or pulp damage due to bacteria penetration or due to cytotoxicity effects of eluted adhesive components, careful consideration of several factors is essential in selecting the suitable bonding procedure and adhesive system for the individual patient situation.

  5. Adhesive behaviour of gecko-inspired nanofibrillar arrays: combination of experiments and finite element modelling

    International Nuclear Information System (INIS)

    Wang Zhengzhi; Xu Yun; Gu Ping

    2012-01-01

    A polypropylene nanofibrillar array was successfully fabricated by template-assisted nanofabrication strategy. Adhesion properties of this gecko-inspired structure were studied through two parallel and independent approaches: experiments and finite element simulations. Experimental results show relatively good normal adhesion, but accompanied by high preloads. The interfacial adhesion was modelled by effective spring elements with piecewise-linear constitution. The effective elasticity of the fibre-array system was originally calculated from our measured elasticity of single nanowire. Comparisons of the experimental and simulative results reveal quantitative agreement except for some explainable deviations, which suggests the potential applicability of the present models and applied theories. (fast track communication)

  6. Hyaluronate acid and oxidized regenerated cellulose prevent adhesion reformation after adhesiolysis in rat models

    Directory of Open Access Journals (Sweden)

    Zhang Y

    2016-10-01

    Full Text Available Yan Zhang, Qin Liu, Ning Yang, Xuegang Zhang Department of Gynecology, Kunshan Hospital Affiliated to Jiangsu University, Kunshan, Jiangsu, People’s Republic of China Abstract: Postsurgical adhesion formation is the most common complication in abdominal and pelvic surgery. Adhesiolysis is the most commonly applied treatment for adhesion formation but is often followed by adhesion reformation. Therefore, an efficient strategy should be adopted to solve these problems. This study aimed to explore whether hyaluronic acid and oxidized regenerated cellulose (ORC could prevent adhesion formation and reformation. Thirty female Sprague Dawley rats were randomly divided into three groups (n=10 each and subjected to different treatments during the first and second surgery. The control group was treated with isotonic sodium chloride, the ORC group was treated with ORC (1.5×1 cm, and the medical sodium hyaluronate (MSH group was treated with 1% MSH (0.5 mL. At 2 weeks after the first surgery, adhesion scores in the MSH group (1.90±0.99 and the ORC group (1.40±0.97 were significantly lower than those in the control group (3.00±0.82 (P=0.005. Similarly, 2 weeks after the second surgery, adhesion scores in the MSH group (2.00±0.82 and the ORC group (1.50±1.27 were significantly lower than those in the control group (3.50±0.53 (P=0.001. In addition, body weights in the MSH group and the ORC group did not change significantly, whereas the control group showed a consistent decrease in body weight during the experiment. Histological examination revealed that inflammatory infiltration was involved in both adhesion formation and reformation. In conclusion, hyaluronic acid and ORC were both efficient in reducing adhesion formation and reformation in the rat model. Keywords: hyaluronic acid, oxidized regenerated cellulose, adhesion formation, adhesion reformation, rat model 

  7. Static friction in elastic adhesive MEMS contacts, models and experiment

    NARCIS (Netherlands)

    Tas, Niels Roelof; Gui, C.; Elwenspoek, Michael Curt

    2000-01-01

    Static friction in shearing mode can be expressed as the product of the shear strength of the interface and the real contact area. The influence of roughness on friction in elastic adhesive contact is analyzed. Special attention is paid to low loading conditions, in which the number of contact

  8. Influence of Blood Contamination During Multimode Adhesive ...

    African Journals Online (AJOL)

    Objectives: The present study evaluated the effects of blood contamination performed at different steps of bonding on the microtensile bond strength (μTBS) of multimode adhesives to dentin when using the self-etch approach. Materials and Methods: Seventy-five molars were randomly assigned to three adhesive groups ...

  9. Strontium effects on root dentin tubule occlusion and nanomechanical properties.

    Science.gov (United States)

    Saeki, Kuniko; Marshall, Grayson W; Gansky, Stuart A; Parkinson, Charles R; Marshall, Sally J

    2016-02-01

    Dentin hypersensitivity often is treated by promotion of dentin tubule occlusion. In this in vitro study we evaluated nanomechanical properties and degree of tubule occlusion conferred to sound and demineralized human root dentin following treatment with a 10% (w/w) strontium acetate solution and its relation to the treatment duration and delivery method. 24 human cervical root dentin disks (8 groups of 3) were polished through 0.25 μm. 12 disks were subjected to an acid challenge (1% citric acid, pH 3.8) for 2 min. The specimens were incubated in artificial saliva, treated by soaking or brushing with deionized (DI) water or a solution of 10% strontium acetate for 2 min twice a day for 28 days. The occlusion percent and nanomechanical properties were determined at the baseline, 5, 14 and 28 days. Cross-sectioned specimens were prepared to evaluate the depth affected by strontium acetate / dentin interaction by SEM. Statistical analysis was performed using linear mixed effects models. A 10% strontium acetate treatment over 5-28 days significantly increased tubule occlusion for normal root dentin and to a lesser extent for demineralized dentin and increased the AFM based nanomechanical properties of demineralized dentin. Brushing was more effective than soaking in recovery of properties of demineralized dentin when treated with strontium. No difference in tubuleocclusion was found between the two delivery methods. Strontium acetate itself proved to have the ability to occlude dentin tubules and result in small changes in the mechanical properties of dentin. Copyright © 2015 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

  10. Gallic Acid Attenuates Postoperative Intra-Abdominal Adhesion by Inhibiting Inflammatory Reaction in a Rat Model

    Science.gov (United States)

    Wei, Guangbing; Wu, Yunhua; Gao, Qi; Shen, Cong; Chen, Zilu; Wang, Kang; Yu, Junhui

    2018-01-01

    Background Intra-abdominal adhesion is one of the most common complications after abdominal surgery. The efficacy of current treatments for intra-abdominal adhesion is unsatisfactory. In this study, we investigated the effect of gallic acid on the prevention and treatment of intra-abdominal adhesions after abdominal surgery using an intra-abdominal adhesion rat model. Material/Methods The experimental rats were randomly divided into the sham operation group, the control group, the chitosan group, and 3 gallic acid groups of different concentrations. All rats except those in the sham operation group received cecal abrasion to induce adhesion. From the first postoperative day, the rats in the gallic acid groups were administered different concentrations of gallic acid in a 2-ml gavage daily. All rats were sacrificed on postoperative day 7, and the degree of intra-abdominal adhesion was evaluated by the naked eye. The amount of collagen deposited between the injured peritoneal tissues was assessed by Sirius red staining. Serum levels of interleukin-6 (IL-6), tumor necrosis factor (TNF-α), and transforming growth factor-β (TGF-β) were measured by ELISA. Western blot was used to detect the level of NF-κB phosphorylation in the injured peritoneal or adhesion tissues of the rats. Results Compared with the control group, the scores of intra-abdominal adhesions in the rats treated with larger doses of gallic acid were significantly decreased, and the degree of inflammation and fibrosis was also significantly decreased. Gallic acid significantly reduced IL-6, TNF-α, and TGF-β1 serum levels. NF-κB phosphorylation in the higher gallic acid groups was significantly reduced. Conclusions Gallic acid inhibits the formation of postoperative intra-abdominal adhesions in rats by inhibiting the inflammatory reaction and fibrogenesis. Gallic acid is a promising drug for preventing intra-abdominal adhesions. PMID:29429982

  11. Probing cellular mechanoadaptation using cell-substrate de-adhesion dynamics: experiments and model.

    Science.gov (United States)

    S S, Soumya; Sthanam, Lakshmi Kavitha; Padinhateeri, Ranjith; Inamdar, Mandar M; Sen, Shamik

    2014-01-01

    Physical properties of the extracellular matrix (ECM) are known to regulate cellular processes ranging from spreading to differentiation, with alterations in cell phenotype closely associated with changes in physical properties of cells themselves. When plated on substrates of varying stiffness, fibroblasts have been shown to exhibit stiffness matching property, wherein cell cortical stiffness increases in proportion to substrate stiffness up to 5 kPa, and subsequently saturates. Similar mechanoadaptation responses have also been observed in other cell types. Trypsin de-adhesion represents a simple experimental framework for probing the contractile mechanics of adherent cells, with de-adhesion timescales shown to scale inversely with cortical stiffness values. In this study, we combine experiments and computation in deciphering the influence of substrate properties in regulating de-adhesion dynamics of adherent cells. We first show that NIH 3T3 fibroblasts cultured on collagen-coated polyacrylamide hydrogels de-adhere faster on stiffer substrates. Using a simple computational model, we qualitatively show how substrate stiffness and cell-substrate bond breakage rate collectively influence de-adhesion timescales, and also obtain analytical expressions of de-adhesion timescales in certain regimes of the parameter space. Finally, by comparing stiffness-dependent experimental and computational de-adhesion responses, we show that faster de-adhesion on stiffer substrates arises due to force-dependent breakage of cell-matrix adhesions. In addition to illustrating the utility of employing trypsin de-adhesion as a biophysical tool for probing mechanoadaptation, our computational results highlight the collective interplay of substrate properties and bond breakage rate in setting de-adhesion timescales.

  12. Combined modeling of cell aggregation and adhesion mediated by receptor–ligand interactions under shear flow

    Directory of Open Access Journals (Sweden)

    Yu Du

    2015-11-01

    Full Text Available Blood cell aggregation and adhesion to endothelial cells under shear flow are crucial to many biological processes such as thrombi formation, inflammatory cascade, and tumor metastasis, in which these cellular interactions are mainly mediated by the underlying receptor–ligand bindings. While theoretical modeling of aggregation dynamics and adhesion kinetics of interacting cells have been well studied separately, how to couple these two processes remains unclear. Here we develop a combined model that couples cellular aggregation dynamics and adhesion kinetics under shear flow. The impacts of shear rate (or shear stress and molecular binding affinity were elucidated. This study provides a unified model where the action of a fluid flow drives cell aggregation and adhesion under the modulations of the mechanical shear flow and receptor–ligand interaction kinetics. It offers an insight into understanding the relevant biological processes and functions.

  13. Cytotoxicity, interaction with dentine and efficacy on multispecies biofilms of a modified salt solution intended for endodontic disinfection in a new in vitro biofilm model.

    Science.gov (United States)

    van der Waal, S V; Scheres, N; de Soet, J J; Wesselink, P R; Crielaard, W

    2015-02-01

    To investigate the cytotoxicity of a modified salt solution (MSS) and evaluate the antimicrobial properties of MSS on in vitro biofilm models. In a metabolic assay, fibroblasts derived from periodontal ligaments (PDL) of human extracted teeth were cultured and challenged with MSS or controls. Then, in active attachment biofilm models, the efficacy of MSS in the presence of dentine powder and in eliminating mature biofilms was investigated. In the dentine assay, a biofilm of Enterococcus faecalis was employed. For the final assay, microorganisms were retrieved from infected root canals and cultured to produce biofilms. After the treatments with MSS or the controls, the biofilms were collected, serially diluted and plated. The colony-forming units were counted. One-way anova was used to analyse the differences between the groups. A P 0.05). In endodontic biofilms, the culturable bacteria were equally reduced by MSS, 2% chlorhexidine (CHX) or 2% sodium hypochlorite (NaOCl) (P > 0.05). Modified salt solution is noncytotoxic in vitro and has good antimicrobial properties equal to CHX and NaOCl. Although the results are promising, ex vivo and in vivo studies are needed before its use as an interappointment root canal dressing can be considered. © 2014 International Endodontic Journal. Published by John Wiley & Sons Ltd.

  14. Papain-based gel for biochemical caries removal: influence on microtensile bond strength to dentin

    Directory of Open Access Journals (Sweden)

    Evandro Piva

    2008-12-01

    Full Text Available This study investigated the influence of a papain-based gel (Papacárie for chemo-mechanical caries removal on bond strength to dentin. Human molars were assigned to the following groups: Group 1: sound teeth were flattened to expose dentin; Group 2: after flattening of surfaces, the papain-based gel was applied on the sound dentin; Group 3: overlying enamel from carious teeth was removed and mechanical excavation of dentin was conducted; Group 4: chemo-mechanical excavation of carious dentin was conducted using the papain-based gel. The Prime&Bond NT or Clearfil SE Bond adhesive systems were used for restorative procedures. A microtensile bond strength test was performed, and the modes of failure were determined under SEM. The data were submitted to two-way ANOVA and Tukey's test (p < 0.05. No significant differences were observed between the sound dentin groups. For both excavation methods, Clearfil presented a significantly higher bond strength than Prime&Bond NT. Also, for Clearfil, the mechanically excavated samples disclosed a significantly higher bond strength than the chemo-mechanically ones. For Prime&Bond NT, no significant differences were detected between the excavation methods. Predominance of mixed failures for the sound substrate and of adhesive failures for the carious dentin one was detected. The bond strength to carious dentin of the self-etching system was negatively affected by chemo-mechanical excavation using the papain-based gel.

  15. Effect of a retention groove on the shear bond strength of dentin-bonded restorations

    NARCIS (Netherlands)

    de Kok, P.; de Jager, N.; Veerman, I.A.M.; Hafeez, N.; Kleverlaan, C.J.; Roeters, J.F.M.

    2016-01-01

    Statement of problem. With the increasing use of minimally invasive restorations, effective adhesion becomes more important. Applying mechanical retention to a flat dentin surface might improve the adhesion of ceramic and composite resin restorations. Purpose. The purpose of this in vitro study was

  16. Coupling biochemistry and mechanics in cell adhesion: a model for inhomogeneous stress fiber contraction

    International Nuclear Information System (INIS)

    Besser, Achim; Schwarz, Ulrich S

    2007-01-01

    Biochemistry and mechanics are closely coupled in cell adhesion. At sites of cell-matrix adhesion, mechanical force triggers signaling through the Rho-pathway, which leads to structural reinforcement and increased contractility in the actin cytoskeleton. The resulting force acts back to the sites of adhesion, resulting in a positive feedback loop for mature adhesion. Here, we model this biochemical-mechanical feedback loop for the special case when the actin cytoskeleton is organized in stress fibers, which are contractile bundles of actin filaments. Activation of myosin II molecular motors through the Rho-pathway is described by a system of reaction-diffusion equations, which are coupled into a viscoelastic model for a contractile actin bundle. We find strong spatial gradients in the activation of contractility and in the corresponding deformation pattern of the stress fiber, in good agreement with experimental findings

  17. Therapeutically Targeting the Inflammasome Product in a Chimeric Model of Endometriosis-Related Surgical Adhesions.

    Science.gov (United States)

    Stocks, Meredith M; Crispens, Marta A; Ding, Tianbing; Mokshagundam, Shilpa; Bruner-Tran, Kaylon L; Osteen, Kevin G

    2017-08-01

    Development of adhesions commonly occurs in association with surgery for endometriosis. Even in the absence of surgery, women with endometriosis appear to be at an enhanced risk of developing adhesions. In the current study, we utilized a chimeric mouse model of experimental endometriosis in order to examine the role of inflammasome activation in the development of postsurgical adhesions. Mice were randomized to receive peritoneal injections of human endometrial tissue fragments or endometrial tissue conditioned media (CM) from women with or without endometriosis 16 hours after ovariectomy and placement of an estradiol-releasing silastic capsule. A subset of mice receiving CM was also treated with interleukin (IL) 1 receptor antagonist (IL-1ra). Our studies demonstrate that peritoneal injection of endometrial tissue fragments near the time of surgery resulted in extensive adhesive disease regardless of tissue origin. However, adhesion scores were significantly higher in mice receiving CM from tissues acquired from patients with endometriosis compared to control tissue CM ( P = .0001). Cytokine bead array analysis of endometrial CM revealed enhanced expression of IL-1β from patients with endometriosis compared to controls ( P endometriosis as a potential causal factor in their increased susceptibility of developing postsurgical adhesions. Thus, targeting inflammasome activation may be an effective strategy for the prevention of surgical adhesions in patients with endometriosis.

  18. Dentin surface treatment using a non-thermal argon plasma brush for interfacial bonding improvement in composite restoration

    Science.gov (United States)

    Ritts, Andy Charles; Li, Hao; Yu, Qingsong; Xu, Changqi; Yao, Xiaomei; Hong, Liang; Wang, Yong

    2010-01-01

    The objective of this study is to investigate the treatment effects of non-thermal atmospheric gas plasmas on dentin surfaces for composite restoration. Extracted unerupted human third molars were used by removing the crowns and etching the exposed dentin surfaces with 35% phosphoric acid gel. The dentin surfaces were treated by using a non-thermal atmospheric argon plasma brush for various durations. The molecular changes of the dentin surfaces were analyzed using FTIR/ATR and an increase in carbonyl groups on dentin surfaces was detected with plasma treated dentin. Adper Single Bond Plus adhesive and Filtek Z250 dental composite were applied as directed. To evaluate the dentin/composite interfacial bonding, the teeth thus prepared were sectioned into micro-bars as the specimens for tensile test. Student Newman Keuls tests showed that the bonding strength of the composite restoration to peripheral dentin was significantly increased (by 64%) after 30 s plasma treatment. However, the bonding strength to plasma treated inner dentin did not show any improvement. It was found that plasma treatment of peripheral dentin surface up to 100 s gave an increase in interfacial bonding strength, while a prolong plasma treatment of dentin surfaces, e.g., 5 min treatments, showed a decrease in interfacial bonding strength. PMID:20831586

  19. Doxycycline-loaded nanotube-modified adhesives inhibit MMP in a dose-dependent fashion.

    Science.gov (United States)

    Palasuk, Jadesada; Windsor, L Jack; Platt, Jeffrey A; Lvov, Yuri; Geraldeli, Saulo; Bottino, Marco C

    2018-04-01

    This article evaluated the drug loading, release kinetics, and matrix metalloproteinase (MMP) inhibition of doxycycline (DOX) released from DOX-loaded nanotube-modified adhesives. DOX was chosen as the model drug, since it is the only MMP inhibitor approved by the U.S. Food and Drug Administration. Drug loading into the nanotubes was accomplished using DOX solution at distinct concentrations. Increased concentrations of DOX significantly improved the amount of loaded DOX. The modified adhesives were fabricated by incorporating DOX-loaded nanotubes into the adhesive resin of a commercial product. The degree of conversion (DC), Knoop microhardness, DOX release kinetics, antimicrobial, cytocompatibility, and anti-MMP activity of the modified adhesives were investigated. Incorporation of DOX-loaded nanotubes did not compromise DC, Knoop microhardness, or cell compatibility. Higher concentrations of DOX led to an increase in DOX release in a concentration-dependent manner from the modified adhesives. DOX released from the modified adhesives did not inhibit the growth of caries-related bacteria, but more importantly, it did inhibit MMP-1 activity. The loading of DOX into the nanotube-modified adhesives did not compromise the physicochemical properties of the adhesives and the released levels of DOX were able to inhibit MMP activity without cytotoxicity. Doxycycline released from the nanotube-modified adhesives inhibited MMP activity in a concentration-dependent fashion. Therefore, the proposed nanotube-modified adhesive may hold clinical potential as a strategy to preserve resin/dentin bond stability.

  20. A simple beam model to analyse the durability of adhesively bonded tile floorings in presence of shrinkage

    Directory of Open Access Journals (Sweden)

    S. de Miranda

    2014-07-01

    Full Text Available A simple beam model for the evaluation of tile debonding due to substrate shrinkage is presented. The tile-adhesive-substrate package is modeled as an Euler-Bernoulli beam laying on a two-layer elastic foundation. An effective discrete model for inter-tile grouting is introduced with the aim of modelling workmanship defects due to partial filled groutings. The model is validated using the results of a 2D FE model. Different defect configurations and adhesive typologies are analysed, focusing the attention on the prediction of normal stresses in the adhesive layer under the assumption of Mode I failure of the adhesive.

  1. Shear bond strength of two bonding systems on dentin surfaces prepared with Er:YAG laser

    International Nuclear Information System (INIS)

    Dall'Magro, Eduardo

    2001-01-01

    The purpose of this study was to examine the shear bond strength of two bonding dentin systems, one 'one step' (Single Bond - 3M) and one 'self-etching' (Prompt-L-ESPE), when applied on dentin surfaces prepared with Er:YAG laser (2,94μm) that underwent ar not, acid etched. Forty one human molars just extracted were selected and after the cut with diamond disc and included in acrylic resin, resulting in 81 specimens (hemi crowns). After, the specimens were divided in one group treated with sand paper and another two groups treated with Er:YAG laser with 200 mJ and 250 mJ of energy and 2 Hz of frequency. Next, the prepared surfaces received three treatments with following application: 1) acid + Single Bond + Z 250 resin, 2) prompt-L-Pop + Z 250 resin, and 3) acid without, Single Bond + Z 250 resin. The Z 250 resin was applied and photopolymerized in increments on a Teflon matrix that belonged to an apparatus called 'Assembly Apparatus' machine producing cylinders of 3,5 mm of diameter and 5 mm of height. After these specimens were submitted to thermo cycling during 1 minute the 55 deg C and during 1 minute with 5 deg C with a total of 500 cycles for specimen, and the measures of shear bond strength were abstained using EMIC model DL 2000 rehearsed machine, with speed of 0,5 mm/min, measuring the final rupture tension (Mpa). The results showed an statistic superiority of 5% of probability level in dentin flattened with sandpaper and with laser using 200 mJ of energy with aspect to the ones flattened with laser using 250 mJ of energy. It was observed that using 'Single Bond' bonding dentin system the marks were statistically superior at 5% of probability with reference to the use of the Prompt-L-Pop adhesive system. So, it was concluded that Er:YAG Laser with 200 mJ of energy produced similar dentin cavity prepare than sandpaper and Single Bond seemed the best bonding agent system between restorative material and dentin. (author)

  2. Mixed-mode fatigue fracture of adhesive joints in harsh environments and nonlinear viscoelastic modeling of the adhesive

    Science.gov (United States)

    Arzoumanidis, Alexis Gerasimos

    A four point bend, mixed-mode, reinforced, cracked lap shear specimen experimentally simulated adhesive joints between load bearing composite parts in automotive components. The experiments accounted for fatigue, solvent and temperature effects on a swirled glass fiber composite adherend/urethane adhesive system. Crack length measurements based on compliance facilitated determination of da/dN curves. A digital image processing technique was also utilized to monitor crack growth from in situ images of the side of the specimen. Linear elastic fracture mechanics and finite elements were used to determine energy release rate and mode-mix as a function of crack length for this specimen. Experiments were conducted in air and in a salt water bath at 10, 26 and 90°C. Joints tested in the solvent were fully saturated. In air, both increasing and decreasing temperature relative to 26°C accelerated crack growth rates. In salt water, crack growth rates increased with increasing temperature. Threshold energy release rate is shown to be the most appropriate design criteria for joints of this system. In addition, path of the crack is discussed and fracture surfaces are examined on three length scales. Three linear viscoelastic properties were measured for the neat urethane adhesive. Dynamic tensile compliance (D*) was found using a novel extensometer and results were considerably more accurate and precise than standard DMTA testing. Dynamic shear compliance (J*) was determined using an Arcan specimen. Dynamic Poisson's ratio (nu*) was extracted from strain gage data analyzed to include gage reinforcement. Experiments spanned three frequency decades and isothermal data was shifted by time-temperature superposition to create master curves spanning thirty decades. Master curves were fit to time domain Prony series. Shear compliance inferred from D* and nu* compared well with measured J*, forming a basis for finding the complete time dependent material property matrix for this

  3. No-waiting dentine self-etch concept-Merit or hype.

    Science.gov (United States)

    Huang, Xue-Qing; Pucci, César R; Luo, Tao; Breschi, Lorenzo; Pashley, David H; Niu, Li-Na; Tay, Franklin R

    2017-07-01

    A recently-launched universal adhesive, G-Premio Bond, provides clinicians with the alternative to use the self-etch technique for bonding to dentine without waiting for the adhesive to interact with the bonding substrate (no-waiting self-etch; Japanese brochure), or after leaving the adhesive undisturbed for 10s (10-s self-etch; international brochure). The present study was performed to examine in vitro performance of this new universal adhesive bonded to human coronal dentine using the two alternative self-etch modes. One hundred and ten specimens were bonded using two self-etch application modes and examined with or without thermomechanical cycling (10,000 thermal cycles and 240,000 mechanical cycles) to simulate one year of intraoral functioning. The bonded specimens were sectioned for microtensile bond testing, ultrastructural and nanoleakage examination using transmission electron microscopy. Changes in the composition of mineralised dentine after adhesive application were examined using Fourier transform infrared spectroscopy. Both reduced application time and thermomechanical cycling resulted in significantly lower bond strengths, thinner hybrid layers, and significantly more extensive nanoleakage after thermomechanical cycling. Using the conventional 10-s application time improved bonding performance when compared with the no-waiting self-etch technique. Nevertheless, nanoleakage was generally extensive under all testing parameters employed for examining the adhesive. Although sufficient bond strength to dentine may be achieved using the present universal adhesive in the no-waiting self-etch mode that does not require clinicians to wait prior to polymerisation of the adhesive, this self-etch concept requires further technological refinement before it can be recommended as a clinical technique. Although the surge for cutting application time to increase user friendliness remains the most frequently sought conduit for advancement of dentine bonding

  4. Bond strength of adhesive resin cement with different adhesive systems

    OpenAIRE

    Lorenzoni e Silva, Fabrizio; Pamato, Saulo; Kuga, Milton-Carlos; S?, Marcus-Vinicius-Reis; Pereira, Jefferson-Ricardo

    2017-01-01

    Background To assess the immediate bond strength of a dual-cure adhesive resin cement to the hybridized dentin with different bonding systems. Material and Methods Fifty-six healthy human molars were randomly divided into 7 groups (n=8). After 3 longitudinal sections, the central cuts were included in PVC matrix and were submitted to dentin hybridization according to the groups: G1 - etch & rinse system with 3-step (Apder? Scotchbond? Multi-Purpose, 3M ESPE), G2 - etch & rinse system with 3-s...

  5. A simplified model for dynamics of cell rolling and cell-surface adhesion

    International Nuclear Information System (INIS)

    Cimrák, Ivan

    2015-01-01

    We propose a three dimensional model for the adhesion and rolling of biological cells on surfaces. We study cells moving in shear flow above a wall to which they can adhere via specific receptor-ligand bonds based on receptors from selectin as well as integrin family. The computational fluid dynamics are governed by the lattice-Boltzmann method. The movement and the deformation of the cells is described by the immersed boundary method. Both methods are fully coupled by implementing a two-way fluid-structure interaction. The adhesion mechanism is modelled by adhesive bonds including stochastic rules for their creation and rupture. We explore a simplified model with dissociation rate independent of the length of the bonds. We demonstrate that this model is able to resemble the mesoscopic properties, such as velocity of rolling cells

  6. Continuum-level modelling of cellular adhesion and matrix production in aggregates.

    Science.gov (United States)

    Geris, Liesbet; Ashbourn, Joanna M A; Clarke, Tim

    2011-05-01

    Key regulators in tissue-engineering processes such as cell culture and cellular organisation are the cell-cell and cell-matrix interactions. As mathematical models are increasingly applied to investigate biological phenomena in the biomedical field, it is important, for some applications, that these models incorporate an adequate description of cell adhesion. This study describes the development of a continuum model that represents a cell-in-gel culture system used in bone-tissue engineering, namely that of a cell aggregate embedded in a hydrogel. Cell adhesion is modelled through the use of non-local (integral) terms in the partial differential equations. The simulation results demonstrate that the effects of cell-cell and cell-matrix adhesion are particularly important for the survival and growth of the cell population and the production of extracellular matrix by the cells, concurring with experimental observations in the literature.

  7. Hyaluronic acid and oxidized regenerated cellulose prevent adhesion reformation after adhesiolysis in rat models

    Science.gov (United States)

    Zhang, Yan; Liu, Qin; Yang, Ning; Zhang, Xuegang

    2016-01-01

    Postsurgical adhesion formation is the most common complication in abdominal and pelvic surgery. Adhesiolysis is the most commonly applied treatment for adhesion formation but is often followed by adhesion reformation. Therefore, an efficient strategy should be adopted to solve these problems. This study aimed to explore whether hyaluronic acid and oxidized regenerated cellulose (ORC) could prevent adhesion formation and reformation. Thirty female Sprague Dawley rats were randomly divided into three groups (n=10 each) and subjected to different treatments during the first and second surgery. The control group was treated with isotonic sodium chloride, the ORC group was treated with ORC (1.5×1 cm), and the medical sodium hyaluronate (MSH) group was treated with 1% MSH (0.5 mL). At 2 weeks after the first surgery, adhesion scores in the MSH group (1.90±0.99) and the ORC group (1.40±0.97) were significantly lower than those in the control group (3.00±0.82) (P=0.005). Similarly, 2 weeks after the second surgery, adhesion scores in the MSH group (2.00±0.82) and the ORC group (1.50±1.27) were significantly lower than those in the control group (3.50±0.53) (P=0.001). In addition, body weights in the MSH group and the ORC group did not change significantly, whereas the control group showed a consistent decrease in body weight during the experiment. Histological examination revealed that inflammatory infiltration was involved in both adhesion formation and reformation. In conclusion, hyaluronic acid and ORC were both efficient in reducing adhesion formation and reformation in the rat model. PMID:27822014

  8. Adaptive Shape Functions and Internal Mesh Adaptation for Modelling Progressive Failure in Adhesively Bonded Joints

    Science.gov (United States)

    Stapleton, Scott; Gries, Thomas; Waas, Anthony M.; Pineda, Evan J.

    2014-01-01

    Enhanced finite elements are elements with an embedded analytical solution that can capture detailed local fields, enabling more efficient, mesh independent finite element analysis. The shape functions are determined based on the analytical model rather than prescribed. This method was applied to adhesively bonded joints to model joint behavior with one element through the thickness. This study demonstrates two methods of maintaining the fidelity of such elements during adhesive non-linearity and cracking without increasing the mesh needed for an accurate solution. The first method uses adaptive shape functions, where the shape functions are recalculated at each load step based on the softening of the adhesive. The second method is internal mesh adaption, where cracking of the adhesive within an element is captured by further discretizing the element internally to represent the partially cracked geometry. By keeping mesh adaptations within an element, a finer mesh can be used during the analysis without affecting the global finite element model mesh. Examples are shown which highlight when each method is most effective in reducing the number of elements needed to capture adhesive nonlinearity and cracking. These methods are validated against analogous finite element models utilizing cohesive zone elements.

  9. Microstructure and wettability of root canal dentine and root canal filling materials after different chemical irrigation

    Energy Technology Data Exchange (ETDEWEB)

    Antonijevic, Djordje; Milovanovic, Petar [Laboratory for Anthropology, Institute for Anatomy, Faculty of Medicine, University of Belgrade, Belgrade (Serbia); Brajkovic, Denis [Department of Dentistry, Faculty of Medical Sciences, University of Kragujevac, Kragujevac (Serbia); Ilic, Dragan [Department of Restorative Medicine, School of Dental Medicine, University of Belgrade, Belgrade (Serbia); Hahn, Michael; Amling, Michael [Department of Osteology and Biomechanics (IOBM), University Medical Center Hamburg-Eppendorf, Lottestr. 55A, 22529 Hamburg (Germany); Rakocevic, Zlatko [Laboratory for Atomic Physics, Institute for Nuclear Science “Vinca”, University of Belgrade, Belgrade (Serbia); Djuric, Marija [Laboratory for Anthropology, Institute for Anatomy, Faculty of Medicine, University of Belgrade, Belgrade (Serbia); Busse, Björn, E-mail: b.busse@uke.uni-hamburg.de [Department of Osteology and Biomechanics (IOBM), University Medical Center Hamburg-Eppendorf, Lottestr. 55A, 22529 Hamburg (Germany)

    2015-11-15

    Graphical abstract: - Highlights: • Different irrigation solutions and disinfectants were used for treatment of root canal dentine and gutta-percha points. • Materials surface characteristics were assessed using quantitative backscattered electron imaging, reference point indentation, and contact angle analyzer. • The most significant differences in mineralization, indentation, and adhesive outcomes were observed after ethylenediaminetetraacetic acid treatment. • Irrigation solutions confer to superior sealing ability of endodontic filling materials. • Micromechanical characteristics of dentine after irrigation are considerable reduced. - Abstract: The objective of this study was to determine the effects of various irrigation solutions on root canal dentine and gutta-percha surface properties. In addition, the effects of disinfectant chemicals on the wettability and surface morphological properties of the filling materials were evaluated. Ethylenediaminetetraacetic acid (EDTA), citric acid, and ozone were employed as irrigation solutions for dentine and gutta-percha treatment. Thereafter, the samples’ microstructure, degree of mineralization, and mechanical properties were assessed by means of quantitative backscattered electron imaging (qBEI) and reference point indentation (RPI). A contact angle analyzer was used to measure adhesion on the tested materials. Here, EDTA had the most significant affect on both the mechanical properties and the adhesive behavior of dentine. Citric acid did not affect dentine wettability, whereas the indentation properties and the mineralization were reduced. Similar effects were observed when ozone was used. The dentinal tubules were significantly widened in citric acid compared to the ozone group. EDTA causes considerable micromechanical surface alteration of dentine and gutta-percha, but represents the best option in clinical cases where a high adhesiveness of the filling materials is desired.

  10. Microstructure and wettability of root canal dentine and root canal filling materials after different chemical irrigation

    International Nuclear Information System (INIS)

    Antonijevic, Djordje; Milovanovic, Petar; Brajkovic, Denis; Ilic, Dragan; Hahn, Michael; Amling, Michael; Rakocevic, Zlatko; Djuric, Marija; Busse, Björn

    2015-01-01

    Graphical abstract: - Highlights: • Different irrigation solutions and disinfectants were used for treatment of root canal dentine and gutta-percha points. • Materials surface characteristics were assessed using quantitative backscattered electron imaging, reference point indentation, and contact angle analyzer. • The most significant differences in mineralization, indentation, and adhesive outcomes were observed after ethylenediaminetetraacetic acid treatment. • Irrigation solutions confer to superior sealing ability of endodontic filling materials. • Micromechanical characteristics of dentine after irrigation are considerable reduced. - Abstract: The objective of this study was to determine the effects of various irrigation solutions on root canal dentine and gutta-percha surface properties. In addition, the effects of disinfectant chemicals on the wettability and surface morphological properties of the filling materials were evaluated. Ethylenediaminetetraacetic acid (EDTA), citric acid, and ozone were employed as irrigation solutions for dentine and gutta-percha treatment. Thereafter, the samples’ microstructure, degree of mineralization, and mechanical properties were assessed by means of quantitative backscattered electron imaging (qBEI) and reference point indentation (RPI). A contact angle analyzer was used to measure adhesion on the tested materials. Here, EDTA had the most significant affect on both the mechanical properties and the adhesive behavior of dentine. Citric acid did not affect dentine wettability, whereas the indentation properties and the mineralization were reduced. Similar effects were observed when ozone was used. The dentinal tubules were significantly widened in citric acid compared to the ozone group. EDTA causes considerable micromechanical surface alteration of dentine and gutta-percha, but represents the best option in clinical cases where a high adhesiveness of the filling materials is desired.

  11. Measurement of conformability and adhesion energy of polymeric ultrathin film to skin model

    Science.gov (United States)

    Sugano, Junki; Fujie, Toshinori; Iwata, Hiroyasu; Iwase, Eiji

    2018-06-01

    We measured the conformability and adhesion energy of a polymeric ultrathin film “nanosheet” with hundreds of nanometer thickness to a skin model with epidermal depressions. To compare the confirmability of the nanosheets with different thicknesses and/or under different attaching conditions, we proposed a measurement method using skin models with the same surface profile and defined the surface strain εS as the quantified value of the conformability. Then, we measured the adhesion energy of the nanosheet at each conformability through a vertical tensile test. Experimental results indicate that the adhesion energy does not depend on the liquid used in wetting the nanosheet before attaching to the skin model and increases monotonously as the surface strain εS increases.

  12. Numerical analysis of single particle impact in the context of Cold Spray: a new adhesion model

    Science.gov (United States)

    Profizi, P.; Combescure, A.; Ogawa, K.

    2016-03-01

    A new adhesion model for numerical simulation of single particle impact in the context of Cold Spray is introduced. As in other studies, cohesive forces are put between the particle and substrate to account for adhesion. In this study however, the forces are put only when a local physical criterion is met. The physical phenomenon most often attributed to Cold Spray adhesion is a shear stress instability. The Johnson-Cook material law is used with a shear damage softening law to enable strong localization at the interface without the need for an extremely fine mesh. This localization is then detected as a drop in local yield stress value by the algorithm, which then implements a local cohesive force. The evolution of this cohesive force is defined by an energy dissipative cohesive model, using a surface adhesion energy as a material parameter. Each cohesive link is broken once all its associated surface energy is dissipated. A criterion on the damage value is also used to break a cohesive bond prematurely, to account for the effect of erosion at higher speeds. This model is found to reproduce the Cold Spray-like adhesion behavior with observed critical and maximum speeds.

  13. Bonding strategies for MIH-affected enamel and dentin.

    Science.gov (United States)

    Krämer, Norbert; Bui Khac, Ngoc-Han Nana; Lücker, Susanne; Stachniss, Vitus; Frankenberger, Roland

    2018-02-01

    Aim of the present study was to evaluate resin composite adhesion to dental hard tissues affected by molar incisor hypomineralisation (MIH). 94 freshly extracted human molars and incisors (53 suffering MIH) were used. 68 teeth (35 with MIH) were used for μ-TBS tests in enamel and dentin, 26 (18 with MIH) for qualitative evaluation. Specimens were bonded with Clearfil SE Bond, Scotchbond Universal, and OptiBond FL. For MIH affected enamel, additional OptiBond FL groups with NaOCl and NaOCl+Icon were investigated. Beside fractographic analysis, also qualitative evaluations were performed using SEM at different magnifications as well as histological sectioning. Highest μ-TBS values were recorded with dentin specimens (ANOVA, mod. LSD, p0.05). Pre-test failures did not occur in dentin specimens. Sound enamel specimens exhibited significantly higher μ-TBS values than MIH enamel (p0.05), however, it caused less pre-test failures (pMIH enamel is the limiting factor in adhesion to MIH teeth. MIH-affected dentin may be bonded conventionally. Copyright © 2017 The Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

  14. Universal adhesives: the next evolution in adhesive dentistry?

    Science.gov (United States)

    Alex, Gary

    2015-01-01

    Every so often a new material, technique, or technological breakthrough spurs a paradigm shift in the way dentistry is practiced. The development and evolution of reliable enamel and dentin bonding agents is one such example. Indeed, the so-called "cosmetic revolution" in dentistry blossomed in large part due to dramatic advances in adhesive technology. It is the ability to bond various materials in a reasonably predictable fashion to both enamel and dentin substrates that enables dentists to routinely place porcelain veneers, direct and indirect composites, and a plethora of other restorative and esthetic materials. In fact, the longevity and predictability of many (if not most) current restorative procedures is wholly predicated on the dentist's ability to bond various materials to tooth tissues. Adhesive systems have progressed from the largely ineffective systems of the 1970s and early 1980s to the relatively successful total- and self-etching systems of today. The latest players in the adhesive marketplace are the so-called "universal adhesives." In theory, these systems have the potential to significantly simplify and expedite adhesive protocols and may indeed represent the next evolution in adhesive dentistry. But what defines a universal system, and are all these new systems truly "universal" and everything they are claimed to be? This article will examine the origin, chemistry, strengths, weaknesses, and clinical relevance of this new genre of dental adhesives.

  15. A phenomenological model of coating/substrate adhesion and interfacial bimetallic peeling stress in composite mirrors

    Science.gov (United States)

    Mcelroy, Paul M.; Lawson, Daniel D.

    1990-01-01

    Adhesion and interfacial stress between metal films and structural composite material substrates is discussed. A theoretical and conceptual basis for selecting coating materials for composite mirror substrates is described. A phenomenological model that interrelates cohesive tensile strength of thin film coatings and interfacial peeling stresses is presented. The model serves as a basis in determining gradiated materials response and compatibility of composite substrate and coating combinations. Parametric evaluation of material properties and geometrical factors such as coating thickness are used to determine the threshold stress levels for maintaining adhesion at the different interfaces.

  16. Elastic-plastic adhesive contact of rough surfaces using n-point asperity model

    International Nuclear Information System (INIS)

    Sahoo, Prasanta; Mitra, Anirban; Saha, Kashinath

    2009-01-01

    This study considers an analysis of the elastic-plastic contact of rough surfaces in the presence of adhesion using an n-point asperity model. The multiple-point asperity model, developed by Hariri et al (2006 Trans ASME: J. Tribol. 128 505-14) is integrated into the elastic-plastic adhesive contact model developed by Roy Chowdhury and Ghosh (1994 Wear 174 9-19). This n-point asperity model differs from the conventional Greenwood and Williamson model (1966 Proc. R. Soc. Lond. A 295 300-19) in considering the asperities not as fixed entities but as those that change through the contact process, and hence it represents the asperities in a more realistic manner. The newly defined adhesion index and plasticity index defined for the n-point asperity model are used to consider the different conditions that arise because of varying load, surface and material parameters. A comparison between the load-separation behaviour of the new model and the conventional one shows a significant difference between the two depending on combinations of mean separation, adhesion index and plasticity index.

  17. Mechanisms of Staphylococcus epidermidis adhesion to model biomaterial surfaces: Establising a link between thrombosis and infection

    Science.gov (United States)

    Higashi, Julie Miyo

    Infections involving Staphylococcus epidermidis remain a life threatening complication associated with the use of polymer based cardiovascular devices. One of the critical steps in infection pathogenesis is the adhesion of the bacteria to the device surface. Currently, mechanisms of S. epidermidis adhesion are incompletely understood, but are thought to involve interactions between bacteria, device surface, and host blood elements in the form of adsorbed plasma proteins and surface adherent platelets. Our central hypothesis is that elements participating in thrombosis also promote S. epidermidis adhesion by specifically binding to the bacterial surface. The adhesion kinetics of S. epidermidis RP62A to host modified model biomaterial surface octadecyltrichlorosilane (OTS) under hydrodynamic shear conditions were characterized. Steady state adhesion to adsorbed proteins and surface adherent platelets was achieved at 90-120 minutes and 60-90 minutes, respectively. A dose response curve of S. epidermidis adhesion in the concentration range of 10sp7{-}10sp9 bac/mL resembled a multilayer adsorption isotherm. Increasing shear stress was found to LTA, and other LTA blocking agents significantly decreased S. epidermidis adhesion to the fibrin-platelet clots, suggesting that this interaction between S. epidermidis and fibrin-platelet clots is specific. Studies evaluated the adhesion of S. epidermidis to polymer immobilized heparin report conflicting results. Paulsson et al., showed that coagulase negative staphylococci adhered in comparable numbers to both immobilized heparin and nonheparinized surfaces, while exhibiting significantly greater adhesion to both surfaces than S. aureus. Preadsorption of the surfaces with specific heparin binding plasma proteins vitronectin, fibronectin, laminin, and collagen significantly increased adhesion. It was postulated that immobilized heparin contained binding sites for the plasma proteins, exposing bacteria binding domains of the

  18. The effect of impaired dentin formation on dental caries:an experimental study in the molars of growing rats

    OpenAIRE

    Huumonen, S. (Sisko)

    1999-01-01

    Abstract The effects of dietary sucrose and systemic glucocorticoid treatment on the response of the pulpodentinal complex to dental caries were examined in an experimental rat model. The possible role of dentinal caries on dentin formation was also examined. After 5-6 weeks of a dietary and/or medication period, the areas of dentin formation and dentinal caries were quantified in the molars of growing animals. Also the number and severity of caries lesions were estimated. Th...

  19. Potential Role of Dentin Sialoprotein by Inducing Dental Pulp Mesenchymal Stem Cell Differentiation and Mineralization for Dental Tissue Repair

    OpenAIRE

    Yuan, Guo-Hua; Yang, Guo-Bin; Wu, Li-An; Chen, Zhi; Chen, Shuo

    2010-01-01

    Introduction: Dentin sialoprotein (DSP) is a dentin extracellular matrix protein, a unique marker of dentinogenesis and plays a vital role in odontoblast differentiation and dentin mineralization. Recently, studies have shown that DSP induces differentiation and mineralization of periodontal ligament stem cells and dental papilla mesenchymal cells in vitro and rescues dentin deficiency and increases enamel mineralization in animal models.The hypothesis: DSP as a nature therapeutic agent stimu...

  20. Human and Bovine Dentin Composition and Its Hybridization Mechanism Assessed by FT-Raman Spectroscopy

    Directory of Open Access Journals (Sweden)

    L. E. S. Soares

    2013-01-01

    Full Text Available FT-Raman spectroscopy was used to study the human and bovine dentin and their interactions with adhesive systems. Ten human (H molars and ten bovine (B teeth were prepared exposing the dentin and then each specimen was divided into two parts. The resulted forty dentin segments were treated either with the total-etch one bottle adhesive (Prime & Bond 2.1, PB or with the single-step self-etching adhesive (Xeno III, X and divided into four groups: HPB (control, HX, BPB, and BX. Each group was analyzed by FT-Raman spectroscopy before and after the adhesive treatment. Six regions of the Raman spectrum were analyzed and the integrated areas of organic and inorganic peaks were calculated. Bovine untreated specimens showed higher peak area of PO4 3−ν2  content than in human specimens. Human untreated specimens had higher peak areas of PO4 3−ν4 and CO3 2−ν1  contents than in bovine specimens. The peak areas of amide III, CH2, and amide I contents were higher in human than in bovine specimens (before treatments. Treated dentin showed no significant statistical differences between the adhesives for both inorganic and organic contents considering the same substrate. However, the differences found between human and bovine specimens after adhesives application show a reduced accuracy of these substrates as a substitute to the human specimens.

  1. Wood : adhesives

    Science.gov (United States)

    A.H. Conner

    2001-01-01

    This chapter on wood adhesives includes: 1) Classification of wood adhesives 2) Thermosetting wood adhesives 3) Thermoplastic adhesives, 4) Wood adhesives based on natural sources 5) Nonconventional bonding of wood 6) Wood bonding.

  2. Influence of irrigation regimens on the adherence of Enterococcus faecalis to root canal dentin.

    Science.gov (United States)

    Kishen, Anil; Sum, Chee-Peng; Mathew, Shibi; Lim, Chwee-Teck

    2008-07-01

    Enterococcus faecalis is frequently associated with post-treatment endodontic infections. Because adherence of bacteria to a substrate is the earliest stage in biofilm formation, eliciting the factors that links adherence of this bacterium to dentin would help in understanding its association with treatment-failed root canals. This investigation aimed to study the effects of endodontic irrigants on the adherence of E. faecalis to dentin. The bacteria adherence assay was conducted by using fluorescence microscopy, and the adhesion force was measured by using atomic force microscopy. There were significant increases in adherence and adhesion force after irrigation of dentin with ethylenediaminetetraacetic acid (EDTA), whereas sodium hypochlorite (NaOCl) reduced it. With the use of chlorhexidine (CHX), the force of adhesion increased, but the adherence assay showed a reduction in the number of adhering bacteria. The irrigation regimen of EDTA, NaOCl, and CHX resulted in the least number of adhering E. faecalis cells. This study highlighted that chemicals that alter the physicochemical properties of dentin will influence the nature of adherence, adhesion force, and subsequent biofilm formation of E. faecalis to dentin.

  3. Comparison of shear bond strength of amalgam bonded to primary and permanent dentin

    Directory of Open Access Journals (Sweden)

    Mahdi S

    2008-06-01

    Full Text Available Amalgam′s non-adhesive characteristics necessitate cavity preparations incorporating retentive features, which often require the removal of non-carious tooth structure. Use of adhesives beneath amalgam restorations, would be helpful to overcome this disadvantage. This study was undertaken to compare the mean shear bond strength of amalgam bonded to primary and permanent dentin, to evaluate the efficacy of amalgam adhesives in pediatric dentistry.27 primary and 28 permanent posterior teeth with intact buccal or lingual surfaces were grounded to expose dentin and wet-polished with 400-grit silicone carbide paper. Scotchbond Multi Purpose Plus adhesive system was applied to the dentin surfaces and light cured. Amalgam was condensed onto the treated dentin through a plastic mold.shear bond strength testing was done using an Instron Universal testing machine, at a crosshead speed of 0.5 mm/min.The data were analyzed by independent samples t-test The difference among the two groups was not statistically significant (p>0.05 Bonded amalgam showed the same level of bond strength to primary and permanent dentin; so, application of amalgam bonding agents in pediatric dentistry can be recommended.

  4. ON THE DURABILITY OF RESIN-DENTIN BONDS: IDENTIFYING THE WEAKEST LINKS

    Science.gov (United States)

    Zhang, Zihou; Beitzel, Dylan; Mutluay, Mustafa; Tay, Franklin R.; Pashley, David H.; Arola, Dwayne

    2015-01-01

    Fatigue of resin-dentin adhesive bonds is critical to the longevity of resin composite restorations. Objectives The objectives were to characterize the fatigue and fatigue crack growth resistance of resin-dentin bonds achieved using two different commercial adhesives and to identify apparent “weak-links”. Methods Bonded interface specimens were prepared using Adper Single Bond Plus (SB) or Adper Scotchbond Multi-Purpose (SBMP) adhesives and 3M Z100 resin composite according to the manufacturers instructions. The stress-life fatigue behavior was evaluated using the twin bonded interface approach and the fatigue crack growth resistance was examined using bonded interface Compact Tension (CT) specimens. Fatigue properties of the interfaces were compared to those of the resin-adhesive, resin composite and coronal dentin. Results The fatigue strength of the SBMP interface was significantly greater than that achieved by SB (p≤0.01). Both bonded interfaces exhibited significantly lower fatigue strength than that of the Z100 and dentin. Regarding the fatigue crack growth resistance, the stress intensity threshold (ΔKth) of the SB interface was significantly greater (p≤0.01) than that of the SBMP, whereas the ΔKth of the interfaces was more than twice that of the parent adhesives. Significance Collagen fibril reinforcement of the resin adhesive is essential to the fatigue crack growth resistance of resin-dentin bonds. Resin tags that are not well hybridized into the surrounding intertubular dentin and/or poor collagen integrity are detrimental to the bonded interface durability. PMID:26169318

  5. Methylene blue 1% solution on the prevention of intraperitoneal adhesion formation in a dog model

    Directory of Open Access Journals (Sweden)

    Marco Augusto Machado Silva

    Full Text Available Intraperitoneal adhesions usually are formed after abdominal surgeries and may cause technical difficulties during surgical intervention, chronic abdominal pain and severe obstructions of the gastrointestinal tract. The current study aimed to evaluate the efficacy of methylene blue (MB 1% solution on the prevention of intraperitoneal postsurgical adhesion formation in a canine surgical trauma model. Twenty bitches were submitted to falciform ligament resection, omentectomy, ovariohysterectomy and scarification of a colonic segment. Prior to abdominal closure, 10 bitches received 1mg kg-1 MB intraperitoneally (MB group and 10 bitches received no treatment (control group, CT. On the 15th postoperative day the bitches were submitted to laparoscopy to assess adhesions. The mean adhesion scores were 13.9 (±5.6 for MB group and 20.5 (±6.4 for the CT group (P=0,043. In conclusion, the 1% MB solution was efficient on the prevention of intraperitoneal postoperative adhesion formation in bitches, especially those involving the colonic serosa.

  6. Modeling cell-substrate de-adhesion dynamics under fluid shear

    Science.gov (United States)

    Maan, Renu; Rani, Garima; Menon, Gautam I.; Pullarkat, Pramod A.

    2018-07-01

    Changes in cell-substrate adhesion are believed to signal the onset of cancer metastasis, but such changes must be quantified against background levels of intrinsic heterogeneity between cells. Variations in cell-substrate adhesion strengths can be probed through biophysical measurements of cell detachment from substrates upon the application of an external force. Here, we investigate, theoretically and experimentally, the detachment of cells adhered to substrates when these cells are subjected to fluid shear. We present a theoretical framework within which we calculate the fraction of detached cells as a function of shear stress for fast ramps as well as the decay in this fraction at fixed shear stress as a function of time. Using HEK and 3T3 fibroblast cells as experimental model systems, we extract characteristic force scales for cell adhesion as well as characteristic detachment times. We estimate force-scales of  ∼500 pN associated to a single focal contact, and characteristic time-scales of s representing cell-spread-area dependent mean first passage times to the detached state at intermediate values of the shear stress. Variations in adhesion across cell types are especially prominent when cell detachment is probed by applying a time-varying shear stress. These methods can be applied to characterizing changes in cell adhesion in a variety of contexts, including metastasis.

  7. Influence of EDC on Dentin-Resin Shear Bond Strength and Demineralized Dentin Thermal Properties

    Directory of Open Access Journals (Sweden)

    Lin Tang

    2016-11-01

    Full Text Available This study aimed to evaluate the bonding strength and thermal properties of demineralized dentin with and without EDC treatment. Sound human molars were randomly divided into seven treatment groups (n = 20: control, 80% ethanol, and five EDC ethanol solutions (0.01–1.0 M. In each group, 16 samples were used for bond strength assessment and 4 samples were used for scanning electron microscopy (SEM analysis. A further 70 intact molars were used to obtain a fine demineralized dentin powder, treated with the same solutions and were evaluated the crosslink degree by ninhydrin test and denaturation temperature (Td by differential scanning calorimetry. EDC-treated specimens (<1.0 M had a higher bond strength, especially 0.3 and 0.5 M group, than the control counterpart. There was a significant drop in bond strength of 1.0 M EDC group. SEM revealed a homogeneous and regular interface under all treatments. EDC treatment significantly increased the demineralized dentin cross-link degree and Td compared with the control and ethanol treatments. The 0.3 and 0.5 M treatments showed the highest cross-link degree and Td. In terms of mechnical and theramal properties consideration, 0.3 and 0.5 M EDC solutions may be favorable for when applied with etch-and-rinse adhesives, but it is still needed further long-term study.

  8. Identification of parameters of cohesive elements for modeling of adhesively bonded joints of epoxy composites

    Directory of Open Access Journals (Sweden)

    Kottner R.

    2013-12-01

    Full Text Available Adhesively bonded joints can be numerically simulated using the cohesive crack model. The critical strain energy release rate and the critical opening displacement are the parameters which must be known when cohesive elements in MSC.Marc software are used. In this work, the parameters of two industrial adhesives Hunstman Araldite 2021 and Gurit Spabond 345 for bonding of epoxy composites are identified. Double Cantilever Beam (DCB and End Notched Flexure (ENF test data were used for the identification. The critical opening displacements were identified using an optimization algorithm where the tests and their numerical simulations were compared.

  9. Modeling cytoskeletal flow over adhesion sites: competition between stochastic bond dynamics and intracellular relaxation

    International Nuclear Information System (INIS)

    Sabass, Benedikt; Schwarz, Ulrich S

    2010-01-01

    In migrating cells, retrograde flow of the actin cytoskeleton is related to traction at adhesion sites located at the base of the lamellipodium. The coupling between the moving cytoskeleton and the stationary adhesions is mediated by the continuous association and dissociation of molecular bonds. We introduce a simple model for the competition between the stochastic dynamics of elastic bonds at the moving interface and relaxation within the moving actin cytoskeleton represented by an internal viscous friction coefficient. Using exact stochastic simulations and an analytical mean field theory, we show that the stochastic bond dynamics lead to biphasic friction laws as observed experimentally. At low internal dissipation, stochastic bond dynamics lead to a regime of irregular stick-and-slip motion. High internal dissipation effectively suppresses cooperative effects among bonds and hence stabilizes the adhesion.

  10. A Histopathological Study of Direct Pulp Capping with Adhesive Resins

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    J. Salhenejad

    2004-12-01

    Full Text Available Statement of Problem: Recently, it has been proposed that different adhesive materials can be used for direct pulp capping. Previous studies have demonstrated that multi steps dentin adhesives could form reparative dentin similar to calcium hydroxide (CH.Purpose: The aim of this study was to evaluate the histological pulp response of ninety mechanically exposed cat pulps to two adhesive resins (Scotch Bond MP and Single Bond 3M were compared with a calcium hydroxide cement (Dycal, Dentsply.Materials and Methods : Class V facial cavities with similar pulpal exposures were prepared in canines. In the experimental groups phosphoric acid was used to etch the enamel and dentin and pulp exposure, and after it dentin adhesives was applied. The exposure point of the control group was capped with Dycal then the remainder of the cavities was etched and a dentin adhesive (single bond was applied. All of the cavities were restored with a composite resin (Z 100 in usual manner. The animals were scarified after 7, 30 and 60 days (n=30, and the pulp evaluated histologically, statistical analysis was carried out with Kruskal- Wallis test (a=0.05.Results: The data showed that most of the cases had mild inflammation of pulp tissue.There was no significant difference in inflammatory reaction of pulp by Dycal and two adhesive systems, severe inflammatory reaction of pulp was observed only in most of the 30- day Single Bond group. Soft tissue organization of dentin bridge was less than ScotchBond and Dycal groups, the differentiation of dentin bridge was less than Scotch Bond group after 7 days.Conclusion: Slight inflammatory cell infiltration was the main reaction of exposed pulp when two commercially available adhesive resins were placed directly on the exposed pulp.There was no significant difference in inflammatory reaction of pulp between Dycal and two adhesive systems after 7 days and 60 days. After 7 days most of the specimens showed an amount of predentin

  11. Adhesion of Model Molecules to Metallic Surfaces, the Implications for Corrosion Protection

    International Nuclear Information System (INIS)

    De Wit, J. H. W.; Van den Brand, J.; De Wit, F. M.; Mol, J. M. C.

    2008-01-01

    The majority of the described experimental results deal with relatively pure aluminium. Variations were made in the pretreatment of the aluminum substrates and an investigation was performed on the resulting changes in oxide layer composition and chemistry. Subsequently, the bonding behavior of the surfaces was investigated by using model adhesion molecules. These molecules were chosen to represent the bonding functionality of an organic polymer. They were applied onto the pretreated surfaces as a monolayer and the bonding behavior was studied using infrared reflection absorption spectroscopy. A direct and clear relation was found between the hydroxyl fraction on the oxide surfaces and the amount of molecules that subsequently bonded to the surface. Moreover, it was found that most bonds between the oxide surface and organic functional groups are not stable in the presence of water. The best performance was obtained using molecules, which are capable of chemisorption with the oxide surface. Finally, it was found that freshly prepared relatively pure aluminum substrates, which are left in air, rapidly lose their bonding capacity towards organic functional groups. This can be attributed to the adsorption of contamination and water to the oxide surface. in addition the adhesion of a typical epoxy-coated aluminum system was investigated during exposure to water at different temperatures. The coating was found to quite rapidly lose its adhesion upon exposure to water. This rapid loss of adhesion corresponds well with the data where it was demonstrated that the studied epoxy coating only bonds through physisorptive hydrogen bonding, these bonds not being stable in the presence of water. After the initial loss the adhesion of the coating was however found to recover again and even exceeded the adhesion prior to exposure. The improvement could be ascribed to the growth of a thin oxyhydroxide layer on the aluminum substrate, which forms a new, water-stable and stronger bond

  12. Ions-modified nanoparticles affect functional remineralization and energy dissipation through the resin-dentin interface.

    Science.gov (United States)

    Toledano, Manuel; Osorio, Raquel; Osorio, Estrella; Medina-Castillo, Antonio Luis; Toledano-Osorio, Manuel; Aguilera, Fátima S

    2017-04-01

    The aim of this study was to evaluate changes in the mechanical and chemical behavior, and bonding ability at dentin interfaces infiltrated with polymeric nanoparticlesstandard deviations and modes of failure are (NPs) prior to resin application. Dentin surfaces were treated with 37% phosphoric acid followed by application of an ethanol suspension of NPs, Zn-NPs or Ca-NPs followed by the application of an adhesive, Single Bond (SB). Bonded interfaces were stored for 24h, submitted to microtensile bond strength test, and evaluated by scanning electron microscopy. After 24h and 21 d of storage, the whole resin-dentin interface adhesive was evaluated using a Nano-DMA. Complex modulus, storage modulus and tan delta (δ) were assessed. AFM imaging and Raman analysis were performed. Bond strength was not affected by NPs infiltration. After 21 d of storage, tan δ generally decreased at Zn-NPs/resin-dentin interface, and augmented when Ca-NPs or non-doped NPs were used. When both Zn-NPs and Ca-NPs were employed, the storage modulus and complex modulus decreased, though both moduli increased at the adhesive and at peritubular dentin after Zn-NPs infiltration. The phosphate and the carbonate peaks, and carbonate substitution, augmented more at interfaces promoted with Ca-NPs than with Zn-NPs after 21 d of storage, but crystallinity did not differ at created interfaces with both ions-doped NPs. Crosslinking of collagen and the secondary structure of collagen improved with Zn-NPs resin-dentin infiltration. Ca-NPs-resin dentin infiltration produced a favorable dissipation of energy with minimal stress concentration trough the crystalline remineralized resin-dentin interface, causing minor damage at this structure. Copyright © 2017 Elsevier Ltd. All rights reserved.

  13. Effect of solvent content on resin hybridization in wet dentin bonding.

    Science.gov (United States)

    Wang, Yong; Spencer, Paulette; Yao, Xiaomei; Brenda, Bohaty

    2007-09-15

    With wet bonding techniques, the channels between the demineralized dentin collagen fibrils are filled with debris, solvent, and water. Commercial adhesives include solvents such as ethanol or acetone to facilitate resin-infiltration into this wet substrate. Under in vivo conditions, the solvent may be diluted because of repeated exposure of the material to the atmosphere, or concentrated because of separation of the bonding liquids into layers within the bottle. The purpose of this study was to investigate the effect of different concentrations of ethanol (10-50%) on infiltration of the adhesive resin and collagen fibril encapsulation in the adhesive/dentin interface using light microscopy, micro-Raman spectroscopy, and scanning electron microscopy. The results indicated that under wet bonding conditions the hybridization process was highly sensitive to the initial solvent concentration in the adhesive system. The staining and scanning electron microscopy results showed that the quality of the interfacial hybrid layer was poor at the lower (10%) or higher (50%) ethanol content. Micro-Raman analysis indicated that there was a distinct difference in the degree of adhesive penetration among adhesives containing different concentrations of ethanol. Adhesives containing 10 or 50% ethanol did not realize effective penetration; the penetration of the adhesive monomers increased dramatically when the initial ethanol content was 30%. The amount of solvents are essential for achieving effective bonding to dentin. Copyright 2007 Wiley Periodicals, Inc.

  14. Localized Modeling of Biochemical and Flow Interactions during Cancer Cell Adhesion.

    Directory of Open Access Journals (Sweden)

    Julie Behr

    Full Text Available This work focuses on one component of a larger research effort to develop a simulation tool to model populations of flowing cells. Specifically, in this study a local model of the biochemical interactions between circulating melanoma tumor cells (TC and substrate adherent polymorphonuclear neutrophils (PMN is developed. This model provides realistic three-dimensional distributions of bond formation and attendant attraction and repulsion forces that are consistent with the time dependent Computational Fluid Dynamics (CFD framework of the full system model which accounts local pressure, shear and repulsion forces. The resulting full dynamics model enables exploration of TC adhesion to adherent PMNs, which is a known participating mechanism in melanoma cell metastasis. The model defines the adhesion molecules present on the TC and PMN cell surfaces, and calculates their interactions as the melanoma cell flows past the PMN. Biochemical rates of reactions between individual molecules are determined based on their local properties. The melanoma cell in the model expresses ICAM-1 molecules on its surface, and the PMN expresses the β-2 integrins LFA-1 and Mac-1. In this work the PMN is fixed to the substrate and is assumed fully rigid and of a prescribed shear-rate dependent shape obtained from micro-PIV experiments. The melanoma cell is transported with full six-degrees-of-freedom dynamics. Adhesion models, which represent the ability of molecules to bond and adhere the cells to each other, and repulsion models, which represent the various physical mechanisms of cellular repulsion, are incorporated with the CFD solver. All models are general enough to allow for future extensions, including arbitrary adhesion molecule types, and the ability to redefine the values of parameters to represent various cell types. The model presented in this study will be part of a clinical tool for development of personalized medical treatment programs.

  15. Localized Modeling of Biochemical and Flow Interactions during Cancer Cell Adhesion.

    Science.gov (United States)

    Behr, Julie; Gaskin, Byron; Fu, Changliang; Dong, Cheng; Kunz, Robert

    2015-01-01

    This work focuses on one component of a larger research effort to develop a simulation tool to model populations of flowing cells. Specifically, in this study a local model of the biochemical interactions between circulating melanoma tumor cells (TC) and substrate adherent polymorphonuclear neutrophils (PMN) is developed. This model provides realistic three-dimensional distributions of bond formation and attendant attraction and repulsion forces that are consistent with the time dependent Computational Fluid Dynamics (CFD) framework of the full system model which accounts local pressure, shear and repulsion forces. The resulting full dynamics model enables exploration of TC adhesion to adherent PMNs, which is a known participating mechanism in melanoma cell metastasis. The model defines the adhesion molecules present on the TC and PMN cell surfaces, and calculates their interactions as the melanoma cell flows past the PMN. Biochemical rates of reactions between individual molecules are determined based on their local properties. The melanoma cell in the model expresses ICAM-1 molecules on its surface, and the PMN expresses the β-2 integrins LFA-1 and Mac-1. In this work the PMN is fixed to the substrate and is assumed fully rigid and of a prescribed shear-rate dependent shape obtained from micro-PIV experiments. The melanoma cell is transported with full six-degrees-of-freedom dynamics. Adhesion models, which represent the ability of molecules to bond and adhere the cells to each other, and repulsion models, which represent the various physical mechanisms of cellular repulsion, are incorporated with the CFD solver. All models are general enough to allow for future extensions, including arbitrary adhesion molecule types, and the ability to redefine the values of parameters to represent various cell types. The model presented in this study will be part of a clinical tool for development of personalized medical treatment programs.

  16. Systemic EP4 Inhibition Increases Adhesion Formation in a Murine Model of Flexor Tendon Repair.

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    Michael B Geary

    Full Text Available Flexor tendon injuries are a common clinical problem, and repairs are frequently complicated by post-operative adhesions forming between the tendon and surrounding soft tissue. Prostaglandin E2 and the EP4 receptor have been implicated in this process following tendon injury; thus, we hypothesized that inhibiting EP4 after tendon injury would attenuate adhesion formation. A model of flexor tendon laceration and repair was utilized in C57BL/6J female mice to evaluate the effects of EP4 inhibition on adhesion formation and matrix deposition during flexor tendon repair. Systemic EP4 antagonist or vehicle control was given by intraperitoneal injection during the late proliferative phase of healing, and outcomes were analyzed for range of motion, biomechanics, histology, and genetic changes. Repairs treated with an EP4 antagonist demonstrated significant decreases in range of motion with increased resistance to gliding within the first three weeks after injury, suggesting greater adhesion formation. Histologic analysis of the repair site revealed a more robust granulation zone in the EP4 antagonist treated repairs, with early polarization for type III collagen by picrosirius red staining, findings consistent with functional outcomes. RT-PCR analysis demonstrated accelerated peaks in F4/80 and type III collagen (Col3a1 expression in the antagonist group, along with decreases in type I collagen (Col1a1. Mmp9 expression was significantly increased after discontinuing the antagonist, consistent with its role in mediating adhesion formation. Mmp2, which contributes to repair site remodeling, increases steadily between 10 and 28 days post-repair in the EP4 antagonist group, consistent with the increased matrix and granulation zones requiring remodeling in these repairs. These findings suggest that systemic EP4 antagonism leads to increased adhesion formation and matrix deposition during flexor tendon healing. Counter to our hypothesis that EP4 antagonism

  17. In vivo remineralization of dentin using an agarose hydrogel biomimetic mineralization system

    Science.gov (United States)

    Han, Min; Li, Quan-Li; Cao, Ying; Fang, Hui; Xia, Rong; Zhang, Zhi-Hong

    2017-02-01

    A novel agarose hydrogel biomimetic mineralization system loaded with calcium and phosphate was used to remineralize dentin and induce the oriented densely parallel packed HA layer on defective dentin surface in vivo in a rabbit model. Firstly, the enamel of the labial surface of rabbits’ incisor was removed and the dentin was exposed to oral environment. Secondly, the hydrogel biomimetic mineralization system was applied to the exposed dentin surface by using a custom tray. Finally, the teeth were extracted and evaluated by scanning electron microscopy, X-ray diffraction, and nanoindentation test after a certain time of mineralization intervals. The regenerated tissue on the dentin surface was composed of highly organised HA crystals. Densely packed along the c axis, these newly precipitated HA crystals were perpendicular to the underlying dental surface with a tight bond. The demineralized dentin was remineralized and dentinal tubules were occluded by the grown HA crystals. The nanohardness and elastic modulus of the regenerated tissue were similar to natural dentin. The results indicated a potential clinical use for repairing dentin-exposed related diseases, such as erosion, wear, and dentin hypersensitivity.

  18. In vitro marginal adaptation of high-viscosity resin composite restorations bonded to dentin cavities.

    Science.gov (United States)

    Rahiotis, Christos; Tzoutzas, John; Kakaboura, Afrodite

    2004-01-01

    The aim of this study was to evaluate the marginal adaptation of high-viscosity resin composite restoratives bonded to dentin in a cylindrical cavity model. The buccal enamel of 64 human premolars was removed and cylindrical cavities 3 mm in diameter and 1.3 mm in depth were prepared on each dentin surface. The cavities were divided into 8 groups of 8 cavities each and restored according to the manufacturers' instructions with the following adhesive/composite systems: Bond 1/Alert, Stae/Glacier, OptiBond Solo/Prodigy Condensable, One-Step/Pyramid, Solidbond/Solitaire, Prime&Bond NT/Surefil, One Coat Bond/Synergy, and Scotchbond 1/Z250. The composite surfaces were pressed against mylar strips, covered with cover slips, and photopolymerized in a single increment for 40 s. The restorations were polished with wet SiC papers of 320 to 1000 grit size to expose dentin margins. The marginal adaptation was evaluated immediately after photopolymerization and again after 1 week of storage in water at 37 +/- 1 degrees C. Evaluation was performed under a metallographic microscope at 200X magnification by recording the frequency of gap-free restorations (GF), the percentage length of the debonded margins relative to the cavity periphery (DM), the width of the maximum marginal gap (MG), and the marginal index (MI = MG x DM / 100). The results were statistically analyzed with one-way ANOVA and the Mann-Whitney U-test at alpha = 0.05. No incidence of gaps was found in 62.5% of One Coat Bond/Synergy and 37.5% of OptiBond Solo/Prodigy Condensable restorations. All the other restorative systems exhibited restorations with gaps. One Coat Bond/Synergy, Scotchbond 1/Z250, and OptiBond Solo/Prodigy Condensable were the groups with the lowest DM values, while Stae/Glacier showed the highest DM values. One Coat Bond/Synergy and OptiBond Solo/Prodigy Condensable revealed the lowest MI values and Stae/Glacier the highest. No statistically significant differences were recorded between

  19. Green tea polyphenol tailors cell adhesivity of RGD displaying surfaces: multicomponent models monitored optically.

    Science.gov (United States)

    Peter, Beatrix; Farkas, Eniko; Forgacs, Eniko; Saftics, Andras; Kovacs, Boglarka; Kurunczi, Sandor; Szekacs, Inna; Csampai, Antal; Bosze, Szilvia; Horvath, Robert

    2017-02-10

    The interaction of the anti-adhesive coating, poly(L-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG) and its Arg-Gly-Asp (RGD) functionalized form, PLL-g-PEG-RGD, with the green tea polyphenol, epigallocatechin-gallate (EGCg) was in situ monitored. After, the kinetics of cellular adhesion on the EGCg exposed coatings were recorded in real-time. The employed plate-based waveguide biosensor is applicable to monitor small molecule binding and sensitive to sub-nanometer scale changes in cell membrane position and cell mass distribution; while detecting the signals of thousands of adhering cells. The combination of this remarkable sensitivity and throughput opens up new avenues in testing complicated models of cell-surface interactions. The systematic studies revealed that, despite the reported excellent antifouling properties of the coatings, EGCg strongly interacted with them, and affected their cell adhesivity in a concentration dependent manner. Moreover, the differences between the effects of the fresh and oxidized EGCg solutions were first demonstrated. Using a semiempirical quantumchemical method we showed that EGCg binds to the PEG chains of PLL-g-PEG-RGD and effectively blocks the RGD sites by hydrogen bonds. The calculations supported the experimental finding that the binding is stronger for the oxidative products. Our work lead to a new model of polyphenol action on cell adhesion ligand accessibility and matrix rigidity.

  20. The effect of dentin on the pulp tissue dissolution capacity of sodium hypochlorite and calcium hydroxide.

    Science.gov (United States)

    Slutzky-Goldberg, Iris; Hanut, Aiham; Matalon, Shlomo; Baev, Valery; Slutzky, Hagay

    2013-08-01

    Sodium hypochlorite (NaOCl) and calcium hydroxide (Ca[OH]2) have tissue dissolution capacity. The aim of this study was to evaluate the potential effect of dentin on their tissue dissolution capacity in a novel dentin model. Dentin models were prepared from 25 freshly extracted human molar teeth; the crowns were separated from the roots, and a rectangular inner shape was prepared. Pulp tissue samples adjusted to similar weights of 6.5 ± 0.2 mg were randomly divided into 6 groups: NaOCl groups in test tubes or dentin models for 1 hour, Ca(OH)2 groups in test tubes or dentin models for 1 week, and control groups saline in test tubes or dentin models for 1 week. The final weights after the experimental period were checked and compared with the initial weights. The differences were statistically analyzed. The tissue dissolution capacity of Ca(OH)2 was affected by the presence of dentin. Similarly, NaOCl lost its effect on the pulp tissue after incubation in dentin. Comparison between all test groups showed highly significant differences (P interactions between local endodontic medicaments, dentin, and pulp tissue. Copyright © 2013 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

  1. Morphology and thickness of the diffusion of resin through demineralized or unconditioned dentinal matrix

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    Arrais César Augusto Galvão

    2002-01-01

    Full Text Available The formation of a hybrid layer is the main bonding mechanism of current dentin-bonding systems. This study evaluated the morphology and thickness of the resin-infiltrated dentinal layer after the application of adhesive systems. The dentin-bonding agents were evaluated on flat dentinal preparations confected on the occlusal surfaces of human teeth. The test specimens were prepared and inspected under scanning electron microscopy at a magnification of X 2,000. The adhesive systems were responsible for different hybrid layer thicknesses (p < 0.05, and the mean values were: for Scotchbond MP Plus (SM, 7.41 ± 1.24mum; for Single Bond (SB, 5.55 ± 0.82mum; for Etch & Prime 3.0 (EP, 3.86 ± 1.17mum; and for Clearfil SE Bond (CB, 1.22 ± 0.45mum. The results suggest that the conventional three-step adhesive system (SM was responsible for the thickest hybrid layer, followed by the one-bottle adhesive (SB. The self-etching adhesives, EP and CB, produced the formation of the thinnest hybrid layers.

  2. Evaluation of matrix metalloproteinase and cysteine cathepsin activity in dentin hybrid layer by gelatin zymography.

    Science.gov (United States)

    Mahalaxmi, Sekar; Madhubala, Manavalan Madhana; Jayaraman, Mahendran; Sathyakumar, Shanmugasundaram

    2016-01-01

    The aim of this study was to comparatively assess the gelatinolytic activity of matrix metalloproteinases(MMPs) and Cysteine Cathepsins (CCs) in the adhesive interface using etch and rinse adhesive at different time intervals using zymographic technique. Twenty freshly extracted non-carious human third molars were used in this study. Occlusal surfaces were ground flat and 1mm thick horizontal dentin slabs were obtained from each tooth using a diamond disc. The dentin surface was polished with 600-grit silicon-carbide paper. Five out of 20 samples were directly pulverized. In the remaining fifteen samples, the dentin was etched and adhesive was applied and light cured according to the manufacturer's instructions. A 1mm thick flowable composite was build up and light cured. Bonded specimens were cut vertically into 3 to 4 dentin slabs by means of diamond disc to expose the adhesive/dentin interfaces. These were then ground down to 500 µm thick resin-dentin interface using a hard tissue microtome. These sections were then pulverised into powder. Following this, every five samples were subjected to zymographic analysis after 1 day, 7 days and 21 days. Zymograms showed clear, thicker bands on all three isoforms in the etched samples compared to control samples at 1st and 7th day intervals and became inactive at 21st day for all three isoforms. MMP 9 activity was relatively higher when compared to CCs and MMP 2. Etch and rinse adhesive activated MMPs and CCs within the hybrid layer that remained active till 7th day and no gelatinolytic activity was found on 21st day and MMPs are more active compared to CCs and MMP-2.

  3. In vitro evaluation of marginal microleakage of class II bonded amalgam restorations using a dentin adhesive and a glass ionomer cement Avaliação in vitro da microinfiltração marginal em restaurações de amálgama tipo classe II usando adesivo dentinário e cimento de ionômero de vidro

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    Edmêr Silvestre PEREIRA JÚNIOR

    1999-04-01

    Full Text Available The purpose of this study was to evaluate in vitro the effectiveness of the dentin bonding system All Bond 2 associated with Resinomer (Bisco, and of Vitrebond (3M glass ionomer cement fresh-mixed, both used in the bonded amalgam technique, to prevent short-term microleakage in class II cavities restored with Dispersalloy (Dentsply, an admixed alloy. The control group utilized the Copalite (Cooley & Cooley varnish. Forty five sound human extracted premolars were used. Class II cavity preparations were made on the mesial and distal surfaces of non-carious teeth, with the gingival margins wall established 1mm under the cementum enamel junction. The specimens were divided randomly into three groups with thirty cavities in each group. The teeth were stored in distilled water for 24 hours and were thermocyled through 500 cycles in distilled water between 5°C and 55°C with a dwell time of 15 seconds. The apices and roots of the teeth were sealed. They were placed in a 37°C bath of 0.5% basic fuchsin dye for 24 hours. The teeth were washed in tap water for 24 hours and cut. The microleakage scores per restoration were averaged and three values of various test groups were subjected to the Kruskal-Wallis and Dunn test at a significance level of p O objetivo deste estudo foi avaliar in vitro a efetividade na prevenção da microinfiltração do sistema adesivo All Bond 2 associado ao Resinomer (Bisco, e do cimento de ionômero de vidro Vitrebond (3M, sem polimerização, em amálgama adesivo classe II, restauradas com Dispersalloy (Dentsply. No grupo controle utilizou-se o verniz cavitário Copalite (Cooley & Cooley. Para tanto, 45 pré-molares humanos íntegros e extraídos, com finalidade ortodôntica, receberam cavidades classe II, sendo uma na face mesial e outra na face distal de cada dente, com a parede cervical localizada a 1mm além da junção cemento-esmalte, sendo 30 cavidades em cada grupo. Após as restaurações os dentes foram estocados

  4. Modeling the formation of cell-matrix adhesions on a single 3D matrix fiber.

    Science.gov (United States)

    Escribano, J; Sánchez, M T; García-Aznar, J M

    2015-11-07

    Cell-matrix adhesions are crucial in different biological processes like tissue morphogenesis, cell motility, and extracellular matrix remodeling. These interactions that link cell cytoskeleton and matrix fibers are built through protein clutches, generally known as adhesion complexes. The adhesion formation process has been deeply studied in two-dimensional (2D) cases; however, the knowledge is limited for three-dimensional (3D) cases. In this work, we simulate different local extracellular matrix properties in order to unravel the fundamental mechanisms that regulate the formation of cell-matrix adhesions in 3D. We aim to study the mechanical interaction of these biological structures through a three dimensional discrete approach, reproducing the transmission pattern force between the cytoskeleton and a single extracellular matrix fiber. This numerical model provides a discrete analysis of the proteins involved including spatial distribution, interaction between them, and study of the different phenomena, such as protein clutches unbinding or protein unfolding. Copyright © 2015 Elsevier Ltd. All rights reserved.

  5. Modelling and Laboratory Studies on the Adhesion Fatigue Performance for Thin-Film Asphalt and Aggregate System

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    Dongsheng Wang

    2014-01-01

    Full Text Available Adhesion between asphalt and aggregate plays an important role in the performance of asphalt mixtures. A low-frequency adhesion fatigue test was proposed in this paper to study the effect of environment on the asphalt-aggregate adhesion system. The stress-based fatigue model had been utilized to describe the fatigue behavior of thin-film asphalt and aggregate system. The factors influencing the adhesion fatigue performance were also investigated. Experiment results show that asphalt has more important effect on the adhesion performance comparing with aggregate. Basalt, which is regarded as hydrophobic aggregates with low silica content, has better adhesion performance to asphalt binder when compared with granite. The effects of aging on the adhesion fatigue performance are different for PG64-22 and rubber asphalt. Long-term aging is found to reduce the adhesion fatigue lives for rubber asphalt and aggregate system, while the effect of long-term aging for aggregate and PG64-22 binder system is positive. Generally the increased stress amplitude and test temperature could induce greater damage and lead to less fatigue lives for adhesion test system.

  6. Predictive modelling of a novel anti-adhesion therapy to combat bacterial colonisation of burn wounds.

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    Paul A Roberts

    2018-05-01

    Full Text Available As the development of new classes of antibiotics slows, bacterial resistance to existing antibiotics is becoming an increasing problem. A potential solution is to develop treatment strategies with an alternative mode of action. We consider one such strategy: anti-adhesion therapy. Whereas antibiotics act directly upon bacteria, either killing them or inhibiting their growth, anti-adhesion therapy impedes the binding of bacteria to host cells. This prevents bacteria from deploying their arsenal of virulence mechanisms, while simultaneously rendering them more susceptible to natural and artificial clearance. In this paper, we consider a particular form of anti-adhesion therapy, involving biomimetic multivalent adhesion molecule 7 coupled polystyrene microbeads, which competitively inhibit the binding of bacteria to host cells. We develop a mathematical model, formulated as a system of ordinary differential equations, to describe inhibitor treatment of a Pseudomonas aeruginosa burn wound infection in the rat. Benchmarking our model against in vivo data from an ongoing experimental programme, we use the model to explain bacteria population dynamics and to predict the efficacy of a range of treatment strategies, with the aim of improving treatment outcome. The model consists of two physical compartments: the host cells and the exudate. It is found that, when effective in reducing the bacterial burden, inhibitor treatment operates both by preventing bacteria from binding to the host cells and by reducing the flux of daughter cells from the host cells into the exudate. Our model predicts that inhibitor treatment cannot eliminate the bacterial burden when used in isolation; however, when combined with regular or continuous debridement of the exudate, elimination is theoretically possible. Lastly, we present ways to improve therapeutic efficacy, as predicted by our mathematical model.

  7. Predictive modelling of a novel anti-adhesion therapy to combat bacterial colonisation of burn wounds.

    Science.gov (United States)

    Roberts, Paul A; Huebinger, Ryan M; Keen, Emma; Krachler, Anne-Marie; Jabbari, Sara

    2018-05-01

    As the development of new classes of antibiotics slows, bacterial resistance to existing antibiotics is becoming an increasing problem. A potential solution is to develop treatment strategies with an alternative mode of action. We consider one such strategy: anti-adhesion therapy. Whereas antibiotics act directly upon bacteria, either killing them or inhibiting their growth, anti-adhesion therapy impedes the binding of bacteria to host cells. This prevents bacteria from deploying their arsenal of virulence mechanisms, while simultaneously rendering them more susceptible to natural and artificial clearance. In this paper, we consider a particular form of anti-adhesion therapy, involving biomimetic multivalent adhesion molecule 7 coupled polystyrene microbeads, which competitively inhibit the binding of bacteria to host cells. We develop a mathematical model, formulated as a system of ordinary differential equations, to describe inhibitor treatment of a Pseudomonas aeruginosa burn wound infection in the rat. Benchmarking our model against in vivo data from an ongoing experimental programme, we use the model to explain bacteria population dynamics and to predict the efficacy of a range of treatment strategies, with the aim of improving treatment outcome. The model consists of two physical compartments: the host cells and the exudate. It is found that, when effective in reducing the bacterial burden, inhibitor treatment operates both by preventing bacteria from binding to the host cells and by reducing the flux of daughter cells from the host cells into the exudate. Our model predicts that inhibitor treatment cannot eliminate the bacterial burden when used in isolation; however, when combined with regular or continuous debridement of the exudate, elimination is theoretically possible. Lastly, we present ways to improve therapeutic efficacy, as predicted by our mathematical model.

  8. Microtensile dentin bond strength of fifth with five seventh-generation dentin bonding agents after thermocycling: An in vitro study

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    Bruhvi Poptani

    2012-01-01

    Full Text Available Objectives: The objective of this in vitro study was to compare the microtensile dentin bond strength (μTBS of five seventh-generation dentin bonding agents (DBA with fifth-generation DBA before and after thermocycling. Materials and Methods: Ten extracted teeth were assigned to fifth generation control group (optibond solo and each of the five experimental groups namely, Group I (G-Bond ,Group II (S 3 Clearfil, Group III (One Coat 7.0, Group IV (Xeno V, and Group V (Optibond all in one. The crown portions of the teeth were horizontally sectioned below the central groove to expose the dentin. The adhesive resins from all groups were bonded to the teeth with their respective composites. Specimens of sizes 1 × 1 × 6 mm 3 were obtained. Fifty specimens that bonded to dentin from each group were selected. Twenty-five of the specimens were tested for debonding without thermocycling and the remaining were subjected to thermocycling followed by μTBS testing. The data were analyzed with one-way ANOVA and Dunnett′s-test for comparison with the reference group(Vth Generation. Results: There was no significant difference (P > 0.05 between the fifth- and seventh-generation adhesives before and after thermocycling. The results of our study showed significantly higher value (P < 0.05 of μTBS of seventh-generation Group II (Clearfil S 3 compared to the fifth-generation before and after thermocycling. Conclusion: The study demonstrated that the Clearfil S 3 bond had the highest μTBS values. In addition, of the five tested seventh-generation adhesive resins were comparable to the fifth-generation DBA.

  9. COHESIVE STRENGTH OF DENTIN RESISTÊNCIA COESIVA DA DENTINA

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    Flávio Fernando DEMARCO

    1997-07-01

    Full Text Available The bond strength of dentin adhesives to dentin has increased after each generation. Although dentin substratum is part of the bonding process, little importance has been given to measure dentin cohesive strength. The aim of this study was to evaluate the cohesive strength of dentin in human canines. Seventeen non carious canines were selected. All of them had been extracted for more than one year. The teeth were ground until dentin square samples with approximately 2 X 2 mm were obtained. They were embedded in acrylic resin and subjected to shear stress, in a Wolpert Machine, at a crosshead speed of 0.5 mm/min. The mean cohesive strength of dentin in shear mode was 33.95 (+-9.72 MPa. The fracture surfaces were observed under a X40 magnification. A finite element analysis was performed to observe the stress distribution as related to the shear test. The failure pattern was compatible with the shear test and also with the stress distribution in the finite element analysisA resistência de união dos adesivos dentinários tem sido aumentada com o desenvolvimento de cada nova geração. Pouca importância tem sido dada à resistência coesiva da dentina. A proposta deste estudo foi avaliar a resistência coesiva da dentina. Dezessete caninos humanos hígidos, os quais tinham sido extraídos há mais de um ano, foram usados. Os dentes foram desgastados até a obtenção de corpos-de-prova em dentina, de formato quadrangular, com tamanho aproximado de 2 X 2 mm. Os dentes foram incluídos em resina acrílica e, então, submetidos ao teste de cisalhamento em uma máquina de ensaios universais Wolpert, com uma velocidade de 0,5 mm/min. A resistência coesiva média da dentina no teste de cisalhamento foi de 33,95 (+- 9,72 MPa. O tipo de fratura foi analisado com um aumento de 40X. Foi realizada uma análise com elemento finito, para observar a distribuição do estresse relacionada com o teste de cisalhamento. O padrão de fratura encontrado foi compat

  10. PVA gel as a potential adhesion barrier: a safety study in a large animal model of intestinal surgery.

    Science.gov (United States)

    Renz, Bernhard W; Leitner, Kurt; Odermatt, Erich; Worthley, Daniel L; Angele, Martin K; Jauch, Karl-Walter; Lang, Reinhold A

    2014-03-01

    Intra-abdominal adhesions following surgery are a major source of morbidity and mortality including abdominal pain and small bowel obstruction. This study evaluated the safety of PVA gel (polyvinyl alcohol and carboxymethylated cellulose gel) on intestinal anastomoses and its potential effectiveness in preventing adhesions in a clinically relevant large animal model. Experiments were performed in a pig model with median laparotomy and intestinal anastomosis following small bowel resection. The primary endpoint was the safety of PVA on small intestinal anastomoses. We also measured the incidence of postoperative adhesions in PVA vs. control groups: group A (eight pigs): stapled anastomosis with PVA gel compared to group B (eight pigs), which had no PVA gel; group C (eight pigs): hand-sewn anastomosis with PVA gel compared to group B (eight pigs), which had no anti-adhesive barrier. Animals were sacrificed 14 days after surgery and analyzed. All anastomoses had a patent lumen without any stenosis. No anastomoses leaked at an intraluminal pressure of 40 cmH2O. Thus, anastomoses healed very well in both groups, regardless of whether PVA was administered. PVA-treated animals, however, had significantly fewer adhesions in the area of stapled anastomoses. The hand-sewn PVA group also had weaker adhesions and trended towards fewer adhesions to adjacent organs. These results suggest that PVA gel does not jeopardize the integrity of intestinal anastomoses. However, larger trials are needed to investigate the potential of PVA gel to prevent adhesions in gastrointestinal surgery.

  11. A review: Biodegradation of resin–dentin bonds

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    Masanori Hashimoto

    2011-02-01

    Full Text Available Resin–dentin bonding was first achieved through mechanical hybridization between resin and collagen fibrils using a functional monomer containing resin system. In the last decade, new adhesive resin systems were frequently released onto the market within a short-period of time. Before and after commercialization, the bond integrity has been tested by bond tests, and leakage evaluation by researchers, but it is very difficult for clinicians to obtain a comprehensive, up-to-date understanding of their nature and degradation. Although newly developed adhesive resins have attempted to improve the bond strength at least in the first 24 h after bonding, the long-term durability of the bonds has not yet been established analytically. However, numerous recent studies have shown micromorphological evidence of biodegradation of resin–dentin bonds, due to hydrolysis of the resin and collagen fibrils within the bonds. This review mainly summarizes the most recent work in biodegradation of resin–dentin bonds based on micromorphological analyses of data obtained by scanning and transmission electron microscopy.

  12. An In Vitro Evaluation of Leakage of Two Etch and Rinse and Two Self-Etch Adhesives after Thermocycling

    Science.gov (United States)

    Geerts, Sabine; Bolette, Amandine; Seidel, Laurence; Guéders, Audrey

    2012-01-01

    Our experiment evaluated the microleakage in resin composite restorations bonded to dental tissues with different adhesive systems. 40 class V cavities were prepared on the facial and lingual surfaces of each tooth with coronal margins in enamel and apical margins in cementum (root dentin). The teeth were restored with Z100 resin composite bonded with different adhesive systems: Scotchbond Multipurpose (SBMP), a 3-step Etch and Rinse adhesive, Adper Scotchbond 1 XT (SB1), a 2-step Etch and Rinse adhesive, AdheSE One (ADSE-1), a 1-step Self-Etch adhesive, and AdheSE (ADSE), a 2-step Self-Etch adhesive. Teeth were thermocycled and immersed in 50% silver nitrate solution. When both interfaces were considered, SBMP has exhibited significantly less microleakage than other adhesive systems (resp., for SB1, ADSE-1 and ADSE, P = 0.0007, P adhesives, microleakage was found greater at enamel than at dentin interfaces (for ADSE, P = 0.024 and for ADSE-1, P adhesive systems, there was no significant difference between enamel and dentin interfaces; (3) SBMP was found significantly better than other adhesives both at enamel and dentin interfaces. In our experiment Etch and Rinse adhesives remain better than Self-Etch adhesives at enamel interface. In addition, there was no statistical difference between 1-step (ADSE-1) and 2-step (ADSE) Self-Etch adhesives. PMID:22675358

  13. Adhesive performance of a multi-mode adhesive system: 1-year in vitro study.

    Science.gov (United States)

    Marchesi, Giulio; Frassetto, Andrea; Mazzoni, Annalisa; Apolonio, Fabianni; Diolosà, Marina; Cadenaro, Milena; Di Lenarda, Roberto; Pashley, David H; Tay, Franklin; Breschi, Lorenzo

    2014-05-01

    The aim of this study was to investigate the adhesive stability over time of a multi-mode one-step adhesive applied using different bonding techniques on human coronal dentine. The hypotheses tested were that microtensile bond strength (μTBS), interfacial nanoleakage expression and matrix metalloproteinases (MMPs) activation are not affected by the adhesive application mode (following the use of self-etch technique or with the etch-and-rinse technique on dry or wet dentine) or by ageing for 24h, 6 months and 1year in artificial saliva. Human molars were cut to expose middle/deep dentine and assigned to one of the following bonding systems (N=15): (1) Scotchbond Universal (3M ESPE) self-etch mode, (2) Scotchbond Universal etch-and-rinse technique on wet dentine, (3) Scotchbond Universal etch-and-rinse technique on dry dentine, and (4) Prime&Bond NT (Dentsply De Trey) etch-and-rinse technique on wet dentine (control). Specimens were processed for μTBS test in accordance with the non-trimming technique and stressed to failure after 24h, 6 months or 1 year. Additional specimens were processed and examined to assay interfacial nanoleakage and MMP expression. At baseline, no differences between groups were found. After 1 year of storage, Scotchbond Universal applied in the self-etch mode and Prime&Bond NT showed higher μTBS compared to the other groups. The lowest nanoleakage expression was found for Scotchbond Universal applied in the self-etch mode, both at baseline and after storage. MMPs activation was found after application of each tested adhesive. The results of this study support the use of the self-etch approach for bonding the tested multi-mode adhesive system to dentine due to improved stability over time. Improved bonding effectiveness of the tested universal adhesive system on dentine may be obtained if the adhesive is applied with the self-etch approach. Copyright © 2014 Elsevier Ltd. All rights reserved.

  14. Shear Bond Strength of Composite to Nd-YAG Lased Dentin with and without Dye

    Directory of Open Access Journals (Sweden)

    H. Kermanshah

    2004-06-01

    Full Text Available Statement of Problem: The achievement of a good and durable dentin/composite resin bond is an important task in restorative dentistry. The application of acid conditioners and dentin bonding agents is an accepted method to enhance this bond strength. Pretreating of dentin surface by laser irradiation seems to be a supplemental way to obtain better results,since lased dentin is more roughened and has a widest surface area to interact with acidconditioner.Purpose: In this study, the effect of dentin surface pretreating by Nd-YAG laser on dentin/composite shear bond strength was examined. Moreover, the effect of Chinese ink as a surface energy absorber on this value was investigated.Methods and Materials: Thirty-nine freshly extracted human teeth without dentinal caries were collected and their occlusal dentins were exposed using a diamond disk. The collected samples were divided into three identical groups. The dentin surface of the first group was lased by an Nd-YAG pulsed laser (100 mJ, 20 Hz through a 320 mm fiber optic in a swiping movement. In the second group, 10% solution of Chinese ink was applied on the dentinal surface before lasing. The samples of the third group were not lased at all. Thedentinal surface prepared by 35% phosphoric acid and Scotchbond MP primer and adhesive. Then, composite resin was cured on dentinal surface. After incubation, in water at 37°C for 24 hours, the samples were tested by Digital Tritest ELE machine.Results: The values of bond strength were 20.83±3.96 MPa, 17.83±3.63 MPa and 19.38±4.88 MPa for the lased, unlased and dye-enhanced groups, respectively. The results were not significant by ANOVA test (a=0.05. Although in the Weiboul modulus, the lased group offered better bond strength.Conclusion: Further studies are required to determine whether chemical as well as physical alterations to the dentin surface are induced by laser etching, and whether these influence the performance of the range of dentin

  15. In vitro Comparative Evaluation of Tensile Bond Strength of 6(th), 7(th) and 8(th) Generation Dentin Bonding Agents.

    Science.gov (United States)

    Kamble, Suresh S; Kandasamy, Baburajan; Thillaigovindan, Ranjani; Goyal, Nitin Kumar; Talukdar, Pratim; Seal, Mukut

    2015-05-01

    Newer dentin bonding agents were developed to improve the quality of composite restoration and to reduce time consumption in its application. The aim of the present study was to evaluate tensile bond strength of 6(th), 7(th) and 8(th) generation bonding agents by in vitro method. Selected 60 permanent teeth were assigned into 20 in each group (Group I: 6(th) generation bonding agent-Adper SE plus 3M ESPE, Group II: 7(th) generation bonding agent-G-Bond GC Corp Japan and Group III: 8(th) generation dentin adhesives-FuturaBond, DC, Voco, Germany). With high-speed diamond disc, coronal dentin was exposed, and selected dentin bonding agents were applied, followed by composite restoration. All samples were saved in saline for 24 h and tensile bond strength testing was done using a universal testing machine. The obtained data were tabulated and statistically analyzed using ANOVA test. The tensile bond strength readings for 6(th) generation bonding agent was 32.2465, for 7(th) generation was 31.6734, and for 8(th)-generation dentine bonding agent was 34.74431. The highest tensile bond strength was seen in 8(th) generation bonding agent compared to 6(th) and 7(th) generation bonding agents. From the present study it can be conclude that 8(th) generation dentine adhesive (Futura DC, Voco, Germany) resulted in highest tensile bond strength compared to 6(th) (Adper SE plus, 3M ESPE) and 7(th) generation (G-Bond) dentin bonding agents.

  16. Marginal adaptation of composite resins under two adhesive techniques.

    Science.gov (United States)

    Dačić, Stefan; Veselinović, Aleksandar M; Mitić, Aleksandar; Nikolić, Marija; Cenić, Milica; Dačić-Simonović, Dragica

    2016-11-01

    In the present research, different adhesive techniques were used to set up fillings with composite resins. After the application of etch and rinse or self etch adhesive technique, marginal adaptation of composite fillings was estimated by the length of margins without gaps, and by the microretention of resin in enamel and dentin. The study material consisted of 40 extracted teeth. Twenty Class V cavities were treated with 35% phosphorous acid and restored after rinsing by Adper Single Bond 2 and Filtek Ultimate-ASB/FU 3M ESPE composite system. The remaining 20 cavities were restored by Adper Easy One-AEO/FU 3M ESPE composite system. Marginal adaptation of composite fillings was examined using a scanning electron microscope (SEM). The etch and rinse adhesive technique showed a significantly higher percentage of margin length without gaps (in enamel: 92.5%, in dentin: 57.3%), compared with the self-etch technique with lower percentage of margin length without gaps, in enamel 70.4% (p resin tugs in interprismatic spaces of enamel, while the dentin microretention was composed of adhesive and hybrid layers with resin tugs in dentin canals. In the second technique, resin tugs were rarely seen and a microgap was dominant along the border of restoration margins. The SEM analysis showed a better marginal adaptation of composite resin to enamel and dentin with better microretention when the etch and rinse adhesive procedure was applied. © 2016 Wiley Periodicals, Inc.

  17. Shear bond strength of self-etch and total-etch bonding systems at different dentin depths

    Directory of Open Access Journals (Sweden)

    Ana Carolina Maito Villela-Rosa

    2011-04-01

    Full Text Available The purpose of this study was to evaluate the dentin shear bond strength of four adhesive systems (Adper Single Bond 2, Adper Prompt L-Pop, Magic Bond DE and Self Etch Bond in regards to buccal and lingual surfaces and dentin depth. Forty extracted third molars had roots removed and crowns bisected in the mesiodistal direction. The buccal and lingual surfaces were fixed in a PVC/acrylic resin ring and were divided into buccal and lingual groups assigned to each selected adhesive. The same specimens prepared for the evaluation of superficial dentin shear resistance were used to evaluate the different depths of dentin. The specimens were identified and abraded at depths of 0.5, 1.0, 1.5 and 2.0 mm. Each depth was evaluated by ISO TR 11405 using an EMIC-2000 machine regulated at 0.5 mm/min with a 200 Kgf load cell. We performed statistical analyses on the results (ANOVA, Tukey and Scheffé tests. Data revealed statistical differences (p < 0.01 in the adhesive and depth variation as well as adhesive/depth interactions. The Adper Single Bond 2 demonstrated the highest mean values of shear bond strength. The Prompt L-Pop product, a self-etching adhesive, revealed higher mean values compared with Magic Bond DE and Self Etch Bond adhesives, a total and self-etching adhesive respectively. It may be concluded that the shear bond strength of dentin is dependent on material (adhesive system, substrate depth and adhesive/depth interaction.

  18. Bond strength of dental adhesive systems irradiated with ionizing radiation.

    Science.gov (United States)

    Dibo da Cruz, Adriana; Goncalves, Luciano de Souza; Rastelli, Alessandra Nara de Souza; Correr-Sobrinho, Lorenco; Bagnato, Vanderlei Salvador; Boscolo, Frab Norberto

    2010-04-01

    The aim of the present paper was to determine the effect of different types of ionizing radiation on the bond strength of three different dentin adhesive systems. One hundred twenty specimens of 60 human teeth (protocol number: 032/2007) sectioned mesiodistally were divided into 3 groups according to the adhesives systems used: SB (Adper Single Bond Plus), CB (Clearfil SE Bond) and AP (Adper Prompt Self-Etch). The adhesives were applied on dentin and photo-activated using LED (Lec 1000, MMoptics, 1000 mW/cm2). Customized elastomer molds (0.5 mm thickness) with three orifices of 1.2 mm diameter were placed onto the bonding areas and filled with composite resin (Filtek Z-250), which was photo-activated for 20 s. Each group was subdivided into 4 subgroups for application of the different types of ionizing radiation: ultraviolet radiation (UV), diagnostic x-ray radiation (DX), therapeutic x-ray radiation (TX) and without irradiation (control group, CG). Microshear tests were carried out (Instron, model 4411), and afterwards the modes of failure were evaluated by optical and scanning electron microscope and classified using 5 scores: adhesive failure, mixed failures with 3 significance levels, and cohesive failure. The results of the shear bond strength test were submitted to ANOVA with Tukey's test and Dunnett's test, and the data from the failure pattern evaluation were analyzed with the Mann Whitney test (p = 0.05). No change in bond strength of CB and AP was observed after application of the different radiation types, only SB showed increase in bond strength after UV (p = 0.0267) irradiation. The UV also changed the failure patterns of SB (p = 0.0001). The radio-induced changes did not cause degradation of the restorations, which means that they can be exposed to these types of ionizing radiation without weakening the bond strength.

  19. Modeling and Adhesive Tool Wear in Dry Drilling of Aluminum Alloys

    International Nuclear Information System (INIS)

    Girot, F.; Gutierrez-Orrantia, M. E.; Calamaz, M.; Coupard, D.

    2011-01-01

    One of the challenges in aeronautic drilling operations is the elimination of cutting fluids while maintaining the quality of drilled parts. This paper therefore aims to increase the tool life and process quality by working on relationships existing between drilling parameters (cutting speed and feed rate), coatings and tool geometry. In dry drilling, the phenomenon of Built-Up Layer is the predominant damage mechanism. A model fitting the axial force with the cutting parameters and the damage has been developed. The burr thickness and its dispersion decrease with the feed rate. The current diamond coatings which exhibit a strong adhesion to the carbide substrate can limit this adhesive layer phenomenon. A relatively smooth nano-structured coating strongly limits the development of this layer.

  20. Comparative study of etched enamel and dentin for the adhesion of composite resins with the Er:YAG 2,94 {mu}m laser and CO{sub 2} 9,6 {mu}m laser: morphological (SEM) and tensile bond strength analysis; Estudo comparativo do condicionamento do esmalte e dentina para a adesao de resinas compostas com os lasers Er:YAG 2,94 {mu}m e com o laser CO{sub 2} de 9,6 {mu}m: analise morfologica e de resistencia a tracao

    Energy Technology Data Exchange (ETDEWEB)

    Marraccini, Tarso Mugnai

    2002-07-01

    The aim of this study was to evaluate and compare the tensile bond strength of a composite resin adhered to the enamel and dentin which have received superficial irradiation with an Er:YAG laser (2.94 {mu}m) or with CO{sub 2} laser ( 9.6 {mu}m) and later on etched with the phosphoric acid at 35%. After the use of the adhesive system, resin cones were made on the etched surfaces by both lasers and tensile bond strength tests were performed. All samples were observed at the SEM - there was an increase of the degree of fusion and resolidification in the irradiated enamel and dentin samples with the CO{sub 2} laser (9.6 {mu}m), creating a vitrified layer with tiny craters. With the Er:YAG laser (2.94 {mu}m) there were typical morphological explosive microablation with the exposition of the tubules in the dentin.The surface acquired by the association of the CO{sub 2} laser ( 9.6 {mu}m) plus acid etching no longer presented the aspect of fusion being this layer completely removed. There were statistical significant differences among ali three methods of etching in the treatment of the enamel and dentin surface. The tensile bond strength test showed that etching of these enamel and dentin surfaces with acid exclusively (control group) presented great values, surpassing the values of the etching acquired with the Er:YAG laser (2.94 {mu}) plus acid or the CO{sub 2} laser (9.6 {mu}m) plus acid. With the parameters used in this experiment the Er:YAG laser (2.94 {mu}m) showed to be more effective than the CO{sub 2} laser (9.6 {mu}m) for the hard dental surfaces etching procedure. (author)

  1. The effect of storage and type of adhesive resin on microleakage of ...

    African Journals Online (AJOL)

    Yomi

    2012-01-16

    Jan 16, 2012 ... Dentin adhesive showed a better durability of the bond to enamel when compared to enamel ... been introduced to improve the bond strength, facilitate and simplify bonding ..... are usually alkenoic acid. This chemical agent.

  2. Dentin-bonding agents

    Directory of Open Access Journals (Sweden)

    João Carlos Gomes

    2008-01-01

    Full Text Available New dental restorative materials have been developed to meet not only the functional demands, but esthetics as well, and in the last few years an enormous range of new materials has appeared for use in dentistry. Among them, several adhesive systems, and different operative techniques for each group materials. Therefore, is indispensable for the professional to know about the properties, characteristics, and association of these materials with the dental structures, in order to select and use them correctly. Should conventional self-etching adhesive systems be used? This question encouraged this literature review to be conducted, with the aim of comparing the conventional adhesive systems with the self-etching systems and to look for scientific data that would help professionals to choose which adhesive system to use. When compared to conventional systems, it was noted that the self-etching systems show less sensitivity to technique, especially as regards errors the operator could commit. The self-etching systems, particularly the 2-step type, have shown equivalent values of bond strength, marginal microleakage and performance, therefore, will be an option for direct composite resin restorations in posterior teeth.

  3. A model-adaptivity method for the solution of Lennard-Jones based adhesive contact problems

    Science.gov (United States)

    Ben Dhia, Hachmi; Du, Shuimiao

    2018-05-01

    The surface micro-interaction model of Lennard-Jones (LJ) is used for adhesive contact problems (ACP). To address theoretical and numerical pitfalls of this model, a sequence of partitions of contact models is adaptively constructed to both extend and approximate the LJ model. It is formed by a combination of the LJ model with a sequence of shifted-Signorini (or, alternatively, -Linearized-LJ) models, indexed by a shift parameter field. For each model of this sequence, a weak formulation of the associated local ACP is developed. To track critical localized adhesive areas, a two-step strategy is developed: firstly, a macroscopic frictionless (as first approach) linear-elastic contact problem is solved once to detect contact separation zones. Secondly, at each shift-adaptive iteration, a micro-macro ACP is re-formulated and solved within the multiscale Arlequin framework, with significant reduction of computational costs. Comparison of our results with available analytical and numerical solutions shows the effectiveness of our global strategy.

  4. Extension of One-Dimensional Models for Hyperelastic String Structures under Coulomb Friction with Adhesion

    Directory of Open Access Journals (Sweden)

    Vladimir Shiryaev

    2018-04-01

    Full Text Available A stretching behavior of knitted and woven textiles is modeled. In our work, the yarns are modeled as one-dimensional hyperelastic strings with frictional contact. Capstan law known for Coulomb’s friction of yarns is extended to an additional adhesion due to gluing of filaments on the yarn surface or some chemical reaction. Two-step Newton’s method is applied for the solution of the large stretching with sliding evolution in the contact nodes. The approach is illustrated on a hysteresis of knitted textile and on the force-strain curve for a woven pattern and both compared with experimental effective curves.

  5. Microleakage after Thermocycling of Three Self-Etch Adhesives under Resin-Modified Glass-Ionomer Cement Restorations

    Directory of Open Access Journals (Sweden)

    Sabine O. Geerts

    2010-01-01

    Full Text Available This study was designed to evaluate microleakage that appeared on Resin-Modified Glass-Ionomer Cement (RMGIC restorations. Sixty class V cavities (h×w×l=2mm×2mm×3mm were cut on thirty extracted third molars, which were randomly allocated to three experimental groups. All the buccal cavities were pretreated with polyacrylic acid, whereas the lingual cavities were treated with three one-step Self-Etch adhesives, respectively, Xeno III (Dentsply Detrey GmbH, Konstanz, Germany, iBond exp (Heraeus Kulzer gmbH & Co. KG, Hanau, Germany, and Adper Prompt-L-Pop (3M ESPE AG, Dental products Seefeld, Germany. All cavities were completely filled with RMGIC, teeth were thermocycled for 800 cycles, and leakage was evaluated. Results were expressed as means ± standard deviations (SDs. Microleakage scores were analysed by means of generalized linear mixed models (GLMMs assuming an ordinal logistic link function. All results were considered to be significant at the 5% critical level (<.05. The results showed that bonding RMGIC to dentin with a Self-Etch adhesive rather than using polyacrylic acid did not influence microleakage scores (=.091, except for one tested Self-Etch adhesive, namely, Xeno III (<.0001. Nevertheless, our results did not show any significant difference between the three tested Self-Etch adhesive systems. In conclusion, the pretreatment of dentin with Self-Etch adhesive system, before RMGIC filling, seems to be an alternative to the conventional Dentin Conditioner for the clinicians as suggested by our results (thermocycling and others (microtensile tests.

  6. Dentin Permeability of Carious Primary Teeth

    African Journals Online (AJOL)

    primary dental pulp make it difficult to determine which modality offers the best ... The most common pathology of the dentine is dental caries. ... to evaluate dentine permeability is to calculate its hydraulic conductance (Lp) using fluid filtration ...

  7. Improved reactive nanoparticles to treat dentin hypersensitivity.

    Science.gov (United States)

    Toledano-Osorio, Manuel; Osorio, Estrella; Aguilera, Fátima S; Luis Medina-Castillo, Antonio; Toledano, Manuel; Osorio, Raquel

    2018-05-01

    The aim of this study was to evaluate the effectiveness of different nanoparticles-based solutions for dentin permeability reduction and to determine the viscoelastic performance of cervical dentin after their application. Four experimental nanoparticle solutions based on zinc, calcium or doxycycline-loaded polymeric nanoparticles (NPs) were applied on citric acid etched dentin, to facilitate the occlusion and the reduction of the fluid flow at the dentinal tubules. After 24 h and 7 d of storage, cervical dentin was evaluated for fluid filtration. Field emission scanning electron microscopy, energy dispersive analysis, AFM and Nano-DMA analysis were also performed. Complex, storage, loss modulus and tan delta (δ) were assessed. Doxycycline-loaded NPs impaired tubule occlusion and fluid flow reduction trough dentin. Tubules were 100% occluded in dentin treated with calcium-loaded NPs or zinc-loaded NPs, analyzed at 7 d. Dentin treated with both zinc-NPs and calcium-NPs attained the highest reduction of dentinal fluid flow. Moreover, when treating dentin with zinc-NPs, complex modulus values attained at intertubular and peritubular dentin were higher than those obtained after applying calcium-NPs. Zinc-NPs are then supposed to fasten active dentin remodeling, with increased maturity and high mechanical properties. Zinc-based nanoparticles are then proposed for effective dentin remineralization and tubular occlusion. Further research to finally prove for clinical benefits in patients with dentin hypersensitivity using Zn-doped nanoparticles is encouraged. Erosion from acids provokes dentin hypersensitivity (DH) which presents with intense pain of short duration. Open dentinal tubules and demineralization favor DH. Nanogels based on Ca-nanoparticles and Zn-nanoparticles produced an efficient reduction of fluid flow. Dentinal tubules were filled by precipitation of induced calcium-phosphate deposits. When treating dentin with Zn-nanoparticles, complex modulus

  8. Effect of EDTA Conditioning and Carbodiimide Pretreatment on the Bonding Performance of All-in-One Self-Etch Adhesives

    Directory of Open Access Journals (Sweden)

    Shipra Singh

    2015-01-01

    Full Text Available Objective. This study evaluated the effect of ethylenediaminetetraacetic acid (EDTA conditioning and carbodiimide (EDC pretreatment on the shear bond strength of two all-in-one self-etch adhesives to dentin. Methods. Flat coronal dentin surfaces were prepared on one hundred and sixty extracted human molars. Teeth were randomly divided into eight groups according to two different self-etch adhesives used [G-Bond and OptiBond-All-In-One] and four different surface pretreatments: (a adhesive applied following manufacturer’s instructions; (b dentin conditioning with 24% EDTA gel prior to application of adhesive; (c EDC pretreatment followed by application of adhesive; (d application of EDC on EDTA conditioned dentin surface followed by application of adhesive. Composite restorations were placed in all the samples. Ten samples from each group were subjected to immediate and delayed (6-month storage in artificial saliva shear bond strength evaluation. Data collected was subjected to statistical analysis using three-way ANOVA and post hoc Tukey’s test at a significance level of p<0.05.  Results and Conclusion. EDTA preconditioning as well as EDC pretreatment alone had no significant effect on the immediate and delayed bond strengths of either of the adhesives. However, EDC pretreatment on EDTA conditioned dentin surface resulted in preservation of resin-dentin bond strength of both adhesives with no significant fall over six months.

  9. The Effect of Phospholipids (Surfactant on Adhesion and Biomechanical Properties of Tendon: A Rat Achilles Tendon Repair Model

    Directory of Open Access Journals (Sweden)

    T. Kursat Dabak

    2015-01-01

    Full Text Available Adhesion of the tendon is a major challenge for the orthopedic surgeon during tendon repair. Manipulation of biological environment is one of the concepts to prevent adhesion. Lots of biochemicals have been studied for this purpose. We aimed to determine the effect of phospholipids on adhesion and biomechanical properties of tendon in an animal tendon repair model. Seventy-two Wistar rats were divided into 4 groups. Achilles tendons of rats were cut and repaired. Phospholipids were applied at two different dosages. Tendon adhesion was determined histopathologically and biomechanical test was performed. At macroscopic evaluation of adhesion, there are statistically significant differences between multiple-dose phospholipid injection group and Control group and also hyaluronic acid group and Control group (p0.008. Ultimate strength was highest at hyaluronic acid injection group and lowest at multiple-dose phospholipid injection group. Single-dose phospholipids (surfactant application may have a beneficial effect on the tendon adhesion. Although multiple applications of phospholipids seem the most effective regime to reduce the tendon adhesion among groups, it deteriorated the biomechanical properties of tendon.

  10. Denture Adhesives

    Science.gov (United States)

    ... Devices Products and Medical Procedures Dental Devices Denture Adhesives Share Tweet Linkedin Pin it More sharing options ... Wearers Reporting Problems to the FDA Background Denture adhesives are pastes, powders or adhesive pads that may ...

  11. Inactivation of local root canal medicaments by dentine: an in vitro study.

    Science.gov (United States)

    Haapasalo, H K; Sirén, E K; Waltimo, T M; Ørstavik, D; Haapasalo, M P

    2000-03-01

    The aim of the study was to investigate the inactivation by dentine of the antibacterial activity of various commonly used local root canal medicaments. The medicaments tested were saturated calcium hydroxide solution, 1% sodium hypochlorite, 0.5% and 0.05% chlorhexidine acetate, and 2/4% and 0.2/0.4% iodine potassium iodide. Dentine was sterilized by autoclaving and crushed into powder with a particle size of 0.2-20 microns. Aliquots of dentine suspension were incubated with the medicaments in sealed test tubes at 37 degrees C for 24 h or 1 h before adding the bacteria. In some experiments bacteria were added simultaneously with dentine powder and the medicament. Enterococcus faecalis A197A was used as a test organism. Samples for bacterial culturing were taken from the suspensions at 5 min, 1 h and 24 h after adding the bacteria. Dentine powder had an inhibitory effect on all medicaments tested. The effect was dependent on the concentration of the medicament as well as on the length of the time the medicament was preincubated with dentine powder before adding the bacteria. The effect of calcium hydroxide on E. faecalis was totally abolished by the presence of dentine powder. Similarly, 0.2/0.4% iodine potassium iodide lost its effect after preincubation for 1 h with dentine before adding the bacteria. The effect of 0.05% chlorhexidine and 1% sodium hypochlorite on E. faecalis was reduced but not totally eliminated by the presence of dentine. No inhibition could be measured when full strength solutions of chlorhexidine and iodine potassium iodide were used in killing E. faecalis. The dentine powder model appears to be an efficient tool for the study of interactions between local endodontic medicaments, dentine, and microbes.

  12. Optimization of the etch-and-rinse technique: New perspectives to improve resin-dentin bonding and hybrid layer integrity by reducing residual water using dimethyl sulfoxide pretreatments.

    Science.gov (United States)

    Stape, Thiago Henrique Scarabello; Tjäderhane, Leo; Abuna, Gabriel; Sinhoreti, Mário Alexandre Coelho; Martins, Luís Roberto Marcondes; Tezvergil-Mutluay, Arzu

    2018-04-13

    To determine whether bonding effectiveness and hybrid layer integrity on acid-etched dehydrated dentin would be comparable to the conventional wet-bonding technique through new dentin biomodification approaches using dimethyl sulfoxide (DMSO). Etched dentin surfaces from extracted sound molars were randomly bonded in wet or dry conditions (30s air drying) with DMSO/ethanol or DMSO/H 2 O as pretreatments using a simplified (Scotchbond Universal Adhesive, 3M ESPE: SU) and a multi-step (Adper Scotchbond Multi-Purpose, 3M ESPE: SBMP) etch-and-rinse adhesives. Untreated dentin surfaces served as control. Bonded teeth (n=8) were stored in distilled water for 24h and sectioned into resin-dentin beams (0.8mm 2 ) for microtensile bond strength test and quantitative interfacial nanoleakage analysis (n=8) under SEM. Additional teeth (n=2) were prepared for micropermeability assessment by CFLSM under simulated pulpar pressure (20cm H 2 O) using 5mM fluorescein as a tracer. Microtensile data was analyzed by 3-way ANOVA followed by Tukey Test and nanoleakage by Kruskal-Wallis and Dunn-Bonferroni multiple comparison test (α=0.05). While dry-bonding of SBMP produced significantly lower bond strengths than wet-bonding (padhesives to demineralized air-dried dentin beyond conventional wet-bonding. Less porous resin-dentin interfaces with higher bond strengths on air-dried etched dentin were achieved; nonetheless, overall efficiency varied according to DMSO's co-solvent and adhesive type. DMSO pretreatments permit etched dentin to be air-dried before hybridization facilitating residual water removal and thus improving bonding effectiveness. This challenges the current paradigm of wet-bonding requirement for the etch-and-rinse approach creating new possibilities to enhance the clinical longevity of resin-dentin interfaces. Copyright © 2018 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved.

  13. The fracture properties and toughening mechanisms of bone and dentin

    Science.gov (United States)

    Koester, Kurt John

    The mechanical properties of bone and dentin and in particular their fracture properties, are the subject of intense research. The relevance of these properties is increasing as our population ages and fracture incidence impacts the lives of a greater portion of the population. A robust framework is needed to understand the fracture properties of bone and dentin to guide researchers as they attempt to characterize the effects of aging, disease, and pharmaceutical treatments on the properties of these mineralized tissues. In the present work, this framework is provided and applied to human bone, human dentin, and animal bone. In situ electron microscopy was also used to identify the salient toughening mechanisms in bone and dentin. It was found that bone and dentin are extrinsically toughened materials and consequently their fracture properties are best characterized utilizing a crack-growth resistance approach. A description of the different mechanical measurements commonly employed when using small animal models (rats and mice) to evaluate the influence of drug therapies on bone fragility is provided. A study where these properties were measured for a large population of wild-type rats and mice was also conducted. Given my findings, it was determined that for the most complete understanding of small animal bone it was necessary to measure strength and toughness. Strength measurements probe the flaw distribution and toughness measurements to evaluate the resistance to facture in the presence of a single dominant worst-case flaw.

  14. Evaluation of inactivation of intracanal antiseptics by dentin, demineralized dentin, dentin matrix and mineral component of dentin

    Directory of Open Access Journals (Sweden)

    Razmi H

    2007-01-01

    Full Text Available Background and Aim: Many studies have shown that microorganisms are the main cause of pulpal diseases and the main purpose of root canal therapy is their elimination from the root canal system. Antiseptic agents are used to reduce bacteria but their antibacterial activities differ from in vivo to in vitro studies and might be inactivated by dentin and its components in root canal space. This study was designed to investigate the effect of dentin on antibacterial activity of different antimicrobial agents. Materials and Methods: In this experimental study, two antibacterial agents (sodium hypochlorite and chlorhexidine with different concentrations were used in four experimental groups: Group 1: dentin, Group 2: demineralized dentin with EDTA, Group 3: dentin matrix and Group 4: dentin mineral component. The species used in this study was Entrococcus faecalis. Different concentration of agents were added to mixture of each experimental group and bacteria. At the baseline and after one and 24 hours, samples were collected and cultured. After incubation period, colonies were counted. Data were analyzed by Tukey test with p<0.05 as the limit of significance. Results: 2% and 0.2% chlorhexidine, and 5% sodium hypochlorite solutions at the three studied times eliminated Entrococcus faecalis completely. 1% sodium hypochlorite eliminated all bacteria in 1h and 24 hs. Statistical analysis showed significant differences between experimental and control groups (P<0.05. Sodium 1% hypochlorite at time 0, could reduce bacteria significantly (P<0.05 but didn’t eliminate them completely. Conclusion: Inactivation of intracanal antiseptics was not observed in this study. As elimination of bacteria occurred, application of these antibacterial agents are recommended in endodontic treatment. Further investigations on other antibacterial agents, other concentrations and shorter time intervals are recommended.

  15. Femtosecond laser ablation of dentin

    International Nuclear Information System (INIS)

    Alves, S; Vilar, R; Oliveira, V

    2012-01-01

    The surface morphology, structure and composition of human dentin treated with a femtosecond infrared laser (pulse duration 500 fs, wavelength 1030 nm, fluences ranging from 1 to 3 J cm -2 ) was studied by scanning electron microscopy, x-ray diffraction, x-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy. The average dentin ablation threshold under these conditions was 0.6 ± 0.2 J cm -2 and the ablation rate achieved in the range 1 to 2 µm/pulse for an average fluence of 3 J cm -2 . The ablation surfaces present an irregular and rugged appearance, with no significant traces of melting, deformation, cracking or carbonization. The smear layer was entirely removed by the laser treatment. For fluences only slightly higher than the ablation threshold the morphology of the laser-treated surfaces was very similar to the dentin fracture surfaces and the dentinal tubules remained open. For higher fluences, the surface was more porous and the dentin structure was partially concealed by ablation debris and a few resolidified droplets. Independently on the laser processing parameters and laser processing method used no sub-superficial cracking was observed. The dentin constitution and chemical composition was not significantly modified by the laser treatment in the processing parameter range used. In particular, the organic matter is not preferentially removed from the surface and no traces of high temperature phosphates, such as the β-tricalcium phosphate, were observed. The achieved results are compatible with an electrostatic ablation mechanism. In conclusion, the high beam quality and short pulse duration of the ultrafast laser used should allow the accurate preparation of cavities, with negligible damage of the underlying material. (paper)

  16. Large deformation analysis of adhesive by Eulerian method with new material model

    International Nuclear Information System (INIS)

    Maeda, K; Nishiguchi, K; Iwamoto, T; Okazawa, S

    2010-01-01

    The material model to describe large deformation of a pressure sensitive adhesive (PSA) is presented. A relationship between stress and strain of PSA includes viscoelasticity and rubber-elasticity. Therefore, we propose the material model for describing viscoelasticity and rubber-elasticity, and extend the presented material model to the rate form for three dimensional finite element analysis. After proposing the material model for PSA, we formulate the Eulerian method to simulate large deformation behavior. In the Eulerian calculation, the Piecewise Linear Interface Calculation (PLIC) method for capturing material surface is employed. By using PLIC method, we can impose dynamic and kinematic boundary conditions on captured material surface. The representative two computational examples are calculated to check validity of the present methods.

  17. Effect of Collagen Matrix Saturation on the Surface Free Energy of Dentin using Different Agents.

    Science.gov (United States)

    de Almeida, Leopoldina de Fátima Dantas; Souza, Samilly Evangelista; Sampaio, Aline Araújo; Cavalcanti, Yuri Wanderley; da Silva, Wander José; Del Bel Cur, Altair A; Hebling, Josimeri

    2015-07-01

    The surface free energy of conditioned-dentin is one of the factors that interfere with monomeric infiltration of the interfibrillar spaces. Saturation of the tooth matrix with different substances may modulate this energy and, consequently, the wettability of the dentin. To evaluate the influence of different substances used to saturate conditioned-dentin on surface free energy (SFE) of this substrate. Dentin blocks (4 × 7 × 1 mm, n = 6/ group), obtained from the roots of bovine incisors, were etched using phosphoric acid for 15 seconds, rinsed and gently dried. The surfaces were treated for 60 seconds with: ultra-purified water (H20-control); ethanol (EtOH), acetone (ACT), chlorhexidine (CHX), ethylenediaminetetraacetic acid (EDTA); or sodium hypochlorite (NaOCl). The tooth surfaces were once again dried with absorbent paper and prepared for SFE evaluation using three standards: water, formamide and bromonaphthalene. Analysis of variance (ANOVA) and Dunnet's tests (a = 0.05) were applied to the data. Ethylenediaminetetraacetic acid was the only substance that caused a change to the contact angle for the standards water and formamide, while only EtOH influenced the angles formed between formamide and the dentin surface. None of the substances exerted a significant effect for bromonaphtha-lene. In comparison to the control, only EDTA and NaOCl altered both polar components of the SFE. Total SFE was increased by saturation of the collagen matrix by EDTA and reduced when NaOCl was used. Saturation of the collagen matrix by EDTA and EtOH changed the surface free energy of the dentin. In addition, the use of NaOCl negatively interfered with the properties evaluated. The increase of surface free energy and wettability of the dentin surface would allow higher penetration of the the adhesive system, which would be of importance to the clinical success of resin-dentin union.

  18. Efficacy of calcium oxide and calcium hydroxide nanoparticles on the elimination of Enterococcus faecalis in human root dentin.

    Science.gov (United States)

    Louwakul, Phumisak; Saelo, Attapon; Khemaleelakul, Saengusa

    2017-04-01

    The objective of this study was to compare the antibacterial effect of calcium oxide nanoparticles (CONPs) and calcium hydroxide nanoparticles (CHNPs) against Enterococcus faecalis in a dentinal block model. E. faecalis strain JCM 7783 was introduced into dentinal tubules of semicylindrical dentin specimens by centrifugation and incubated for 1 week. Fifty microliters of CONPs or CHNPs was placed on the root canal side of the infected dentin specimens. The specimens were then incubated in aerobic condition at 37 °C and 100 % relative humidity for 1 week. The treated dentin specimens were subjected to fluorescent staining and confocal laser scanning microscopy (CLSM) to analyze the proportions of non-vital and vital bacterial cells inside the dentinal tubules. Scanning electron microscopy (SEM) was used to confirm the effect of the medicaments on the bacteria in the dentinal tubules. Calcium oxide (CO) and calcium hydroxide (CH) were used as controls. Based on the CLSM and SEM analyses, CHNPs were more efficient than CONPs in the elimination of the bacteria in the dentinal tubules. CONPs significantly killed more E. faecalis than CO and CH (P < .05). Neither CO nor CH was able to kill the bacteria. CHNPs were more effective than CONPs in the elimination of E. faecalis in dentinal tubules. CHNPs are effective nanoparticles in killing endodontic bacteria present in dentinal tubules. They have potential as an intracanal medicament, which may be beneficial in root canal therapy.

  19. Studies on Mathematical Models of Wet Adhesion and Lifetime Prediction of Organic Coating/Steel by Grey System Theory.

    Science.gov (United States)

    Meng, Fandi; Liu, Ying; Liu, Li; Li, Ying; Wang, Fuhui

    2017-06-28

    A rapid degradation of wet adhesion is the key factor controlling coating lifetime, for the organic coatings under marine hydrostatic pressure. The mathematical models of wet adhesion have been studied by Grey System Theory (GST). Grey models (GM) (1, 1) of epoxy varnish (EV) coating/steel and epoxy glass flake (EGF) coating/steel have been established, and a lifetime prediction formula has been proposed on the basis of these models. The precision assessments indicate that the established models are accurate, and the prediction formula is capable of making precise lifetime forecasting of the coatings.

  20. Model for adhesion clutch explains biphasic relationship between actin flow and traction at the cell leading edge

    Science.gov (United States)

    Craig, Erin M.; Stricker, Jonathan; Gardel, Margaret L.; Mogilner, Alex

    2015-01-01

    Cell motility relies on the continuous reorganization of a dynamic actin-myosin-adhesion network at the leading edge of the cell, in order to generate protrusion at the leading edge and traction between the cell and its external environment. We analyze experimentally measured spatial distributions of actin flow, traction force, myosin density, and adhesion density in control and pharmacologically perturbed epithelial cells in order to develop a mechanical model of the actin-adhesion-myosin self-organization at the leading edge. A model in which the F-actin network is treated as a viscous gel, and adhesion clutch engagement is strengthened by myosin but weakened by actin flow, can explain the measured molecular distributions and correctly predict the spatial distributions of the actin flow and traction stress. We test the model by comparing its predictions with measurements of the actin flow and traction stress in cells with fast and slow actin polymerization rates. The model predicts how the location of the lamellipodium-lamellum boundary depends on the actin viscosity and adhesion strength. The model further predicts that the location of the lamellipodium-lamellum boundary is not very sensitive to the level of myosin contraction. PMID:25969948

  1. Effect of desensitizing toothpastes on dentin

    Directory of Open Access Journals (Sweden)

    Shelon Cristina Souza Pinto

    2012-10-01

    Full Text Available The objective of this study was to analyze the effects of toothbrushing with desensitizing toothpastes on dentin permeability and dentinal tubule occlusion. Fifty rats provided two hundred incisor teeth divided into five groups: DW, brushed with distilled water (control; FT, brushed with fluoride toothpaste; SCT, brushed with strontium chloride toothpaste; PCT, brushed with potassium citrate toothpaste; and PNT, brushed with potassium nitrate toothpaste. Cavities were prepared to expose the dentinal tubules, and the incisor teeth were brushed using the experimental agents. After each treatment, Evans blue dye solution was applied to the teeth. Dentin permeability was analyzed using scanning electron microscopy and energy-dispersive X-rays (EDX. There were significant differences (p < 0.0001, ANOVA among the groups regarding dentin permeability, number of dentinal tubules, diameter of dentinal tubules, and opened tubular area. In the SCT, PCT and PNT groups, opened and partially occluded tubules, deposits, and a few smear layers were observed. In the DW and FT groups, most of the dentinal tubules were open, with no deposits or smear layers on the dentin. EDX revealed peaks of calcium and phosphorus in all of the groups, as well as traces of strontium in the SCT group and of potassium in the PCT and PNT groups. Desensitizing toothpaste decreased dentin permeability, although it produced only partial dentin tubule occlusion.

  2. Ex Vivo and In Vivo Mice Models to Study Blastocystis spp. Adhesion, Colonization and Pathology: Closer to Proving Koch's Postulates.

    Directory of Open Access Journals (Sweden)

    Sitara S R Ajjampur

    Full Text Available Blastocystis spp. are widely prevalent extra cellular, non-motile anerobic protists that inhabit the gastrointestinal tract. Although Blastocystis spp. have been associated with gastrointestinal symptoms, irritable bowel syndrome and urticaria, their clinical significance has remained controversial. We established an ex vivo mouse explant model to characterize adhesion in the context of tissue architecture and presence of the mucin layer. Using confocal microscopy with tissue whole mounts and two axenic isolates of Blastocystis spp., subtype 7 with notable differences in adhesion to intestinal epithelial cells (IEC, isolate B (ST7-B and isolate H (more adhesive, ST7-H, we showed that adhesion is both isolate dependent and tissue trophic. The more adhesive isolate, ST7-H was found to bind preferentially to the colon tissue than caecum and terminal ileum. Both isolates were also found to have mucinolytic effects. We then adapted a DSS colitis mouse model as a susceptible model to study colonization and acute infection by intra-caecal inoculation of trophic Blastocystis spp.cells. We found that the more adhesive isolate ST7-H was also a better colonizer with more mice shedding parasites and for a longer duration than ST7-B. Adhesion and colonization was also associated with increased virulence as ST7-H infected mice showed greater tissue damage than ST7-B. Both the ex vivo and in vivo models used in this study showed that Blastocystis spp. remain luminal and predominantly associated with mucin. This was further confirmed using colonic loop experiments. We were also successfully able to re-infect a second batch of mice with ST7-H isolates obtained from fecal cultures and demonstrated similar histopathological findings and tissue damage thereby coming closer to proving Koch's postulates for this parasite.

  3. Effect of evaporation on the shelf life of a universal adhesive.

    Science.gov (United States)

    Pongprueksa, P; Miletic, V; De Munck, J; Brooks, N R; Meersman, F; Nies, E; Van Meerbeek, B; Van Landuyt, K L

    2014-01-01

    The purpose of this study was to evaluate how evaporation affects the shelf life of a one-bottle universal adhesive. Three different versions of Scotchbond Universal (SBU, 3M ESPE, Seefeld, Germany) were prepared using a weight-loss technique. SBU0 was left open to the air until maximal weight loss was obtained, whereas SBU50 was left open until 50% of evaporation occurred. In contrast, SBU100 was kept closed and was assumed to contain the maximum concentration of all ingredients. The degree of conversion (DC) was determined by using Fourier transform infrared spectroscopy on different substrates (on dentin or glass plate and mixed with dentin powder); ultimate microtensile strength and microtensile bond strength to dentin were measured as well. DC of the 100% solvent-containing adhesive (SBU100) was higher than that of the 50% (SBU50) and 0% (SBU0) solvent-containing adhesives for all substrates. DC of the adhesive applied onto glass and dehydrated dentin was higher than that applied onto dentin. Even though the ultimate microtensile strength of SBU0 was much higher than that of SBU50 and SBU100, its bond strength to dentin was significantly lower. Evaporation of adhesive ingredients may jeopardize the shelf life of a one-bottle universal system by reducing the degree of conversion and impairing bond strength. However, negative effects only became evident after more than 50% evaporation.

  4. Dentin-cement Interfacial Interaction

    Science.gov (United States)

    Atmeh, A.R.; Chong, E.Z.; Richard, G.; Festy, F.; Watson, T.F.

    2012-01-01

    The interfacial properties of a new calcium-silicate-based coronal restorative material (Biodentine™) and a glass-ionomer cement (GIC) with dentin have been studied by confocal laser scanning microscopy (CLSM), scanning electron microscopy (SEM), micro-Raman spectroscopy, and two-photon auto-fluorescence and second-harmonic-generation (SHG) imaging. Results indicate the formation of tag-like structures alongside an interfacial layer called the “mineral infiltration zone”, where the alkaline caustic effect of the calcium silicate cement’s hydration products degrades the collagenous component of the interfacial dentin. This degradation leads to the formation of a porous structure which facilitates the permeation of high concentrations of Ca2+, OH-, and CO32- ions, leading to increased mineralization in this region. Comparison of the dentin-restorative interfaces shows that there is a dentin-mineral infiltration with the Biodentine, whereas polyacrylic and tartaric acids and their salts characterize the penetration of the GIC. A new type of interfacial interaction, “the mineral infiltration zone”, is suggested for these calcium-silicate-based cements. PMID:22436906

  5. Non-contact adhesion to self-affine surfaces: A theoretical model

    Energy Technology Data Exchange (ETDEWEB)

    Makeev, Maxim A., E-mail: makeev@umich.edu

    2013-11-22

    Strength of adhesion between materials is known to be strongly influenced by interface irregularities. In this work, I devise a perturbative approach to describe the effect of self-affine roughness on non-contact adhesive interactions. The hierarchy of the obtained analytical solutions is the following. First, analytical formulae are deduced to describe roughness corrections to the van der Waals interaction energies between a hemi-space adherend, bounded by a self-affine surface, and a point-like adherent. Second, the problem of two hemi-spaces, one of which has a planar surface, and the other is bounded by a self-affine surface, is solved analytically. In the latter case, a numerical analysis is performed to delineate the behavior of the roughness corrections as a function of the parameters, characterizing self-affine fractal surface roughness. The problem of two hemi-spaces, both bounded by self-affine fractal surfaces, is also addressed in this work. The model's predictions are compared with previously reported theoretical results and available experimental data.

  6. Extrafibrillar collagen demineralization-based chelate-and-rinse technique bridges the gap between wet and dry dentin bonding.

    Science.gov (United States)

    Mai, Sui; Wei, Chin-Chuan; Gu, Li-Sha; Tian, Fu-Cong; Arola, Dwayne D; Chen, Ji-Hua; Jiao, Yang; Pashley, David H; Niu, Li-Na; Tay, Franklin R

    2017-07-15

    Limitations associated with wet-bonding led to the recent development of a selective demineralization strategy in which dentin was etched with a reduced concentration of phosphoric acid to create exclusive extrafibrillar demineralization of the collagen matrix. However, the use of acidic conditioners removes calcium via diffusion of very small hydronium ions into the intrafibrillar collagen water compartments. This defeats the purpose of limiting the conditioner to the extrafibrillar space to create a collagen matrix containing only intrafibrillar minerals to prevent collapse of the collagen matrix. The present work examined the use of polymeric chelators (the sodium salt of polyacrylic acid) of different molecular weights to selectively demineralize extrafibrillar dentin. These polymeric chelators exhibit different affinities for calcium ions (isothermal titration calorimetry), penetrated intrafibrillar dentin collagen to different extents based on their molecular sizes (modified size-exclusion chromatography), and preserve the dynamic mechanical properties of mineralized dentin more favorably compared with completely demineralized phosphoric acid-etched dentin (nanoscopical dynamic mechanical analysis). Scanning and transmission electron microscopy provided evidence for retention of intrafibrillar minerals in dentin surfaces conditioned with polymeric chelators. Microtensile bond strengths to wet-bonded and dry-bonded dentin conditioned with these polymeric chelators showed that the use of sodium salts of polyacrylic acid for chelating dentin prior to bonding did not result in significant decline in resin-dentin bond strength. Taken together, the findings led to the conclusion that a chelate-and-rinse conditioning technique based on extrafibrillar collagen demineralization bridges the gap between wet and dry dentin bonding. The chelate-and-rinse dental adhesive bonding concept differentiates from previous research in that it is based on the size

  7. Sealing properties of a self-etching primer system to normal caries-affected and caries-infected dentin.

    Science.gov (United States)

    Lee, Kwang-Won; Son, H-H; Yoshiyama, Masatoshi; Tay, Franklin R; Carvalho, Ricardo M; Pashley, David H

    2003-09-01

    To compare the ability of an experimental antibacterial self-etching primer adhesive system to seal exposure sites in normal, caries-affected and caries-infected human dentin. 30 extracted human third molars were used within 1 month of extraction. 10 intact normal teeth comprised the normal group. 20 teeth with occlusal caries that radiographically extended halfway to the pulp were excavated using caries-detector solution (CDS) and a #4 round carbide bur in a slowspeed handpiece. Half of those teeth were fully excavated free of CDS-stained material without exposing the pulp, and were designated as the caries-affected dentin group. The remaining 10 teeth were excavated as close to the pulp as possible without obtaining an exposure, but whose dentin continued to stain red with CDS; this group was designated as the caries-infected dentin group. The remaining dentin thickness in all of the specimens in the other two groups was then reduced to the same extent as the caries-infected group. Direct exposures of the pulp chamber were made with a 1/4 round bur in the normal dentin or a 25 gauge needle in the other two groups. After measuring the fluid flow through the exposure, the sites were then sealed with an experimental antibacterial fluoride-containing self-etching primer adhesive systems (ABF). Fluid conductance was remeasured every week for 16 weeks. The fluid conductance through the exposure fell 99% in all groups following resin sealing. The seals of normal and caries-affected dentin remained relatively stable over the 16 weeks, while the seals of caries-infected dentin gradually deteriorated, reaching significance at 8 weeks. TEM examination revealed very thin (ca. 0.5 mm) hybrid layers in normal dentin, 3-4 microm thick hybrid layers in caries-affected dentin and 40 microm thick hybrid layers in caries-infected dentin. The tubules of caries-infected dentin were enlarged and filled with bacteria. Resin tags passed around these bacteria in the top 20-40 microm

  8. Effect of Different Saliva Decontamination Procedures on Bond Strength to Dentin in Single Bottle Systems

    Directory of Open Access Journals (Sweden)

    M. Ghavam

    2004-09-01

    Full Text Available Statement of Problem: Following the increasing use of composites in restoring anterior and posterior teeth, problems due to its technique sensitivity have become a major concern.One of these problems is the possibility of contamination of dentin with saliva, blood and/or gingival fluid in different stages of bonding procedure, even with application of different methods of isolation. However, by introduction of Single-bottle dentin adhesives,the contamination possibility reduced to two stages. Scientific documents show that saliva contamination reduces bond strength of composites to dentin. Application of simple and efficient methods for reducing or eliminating saliva contamination enables clinicians to carry out dental treatment without any concern about deterioration of clinical longevity of restoration.Purpose: This study was designed to compare the effect of different decontamination methods on the shear bond strength of composite to dentin using a “Single-bottle” adhesive.Materials and Methods: Seventy-two extracted sound human molars and premolars were selected. Enamel of buccal surface was ground flat to expose dentin. The teeth were divided into 9 groups of 8 each. In control group (1 the adhesive “Excite” was used according tothe manufacturer, without any contamination. Conditioned and saliva contaminated dentin was (2 rinsed and blot dried, (3 rinsed, dried and re-etched. In groups 4, 5, 6 uncured adhesive was saliva contaminated and then: (4 only blot dried (5 rinsed, blot dried with adhesive reapplication and (6 resurfaced with bur, rinsed, dried and followed by repeating the whole process. In groups 7, 8, 9 cured adhesive was contaminated with saliva and then:(7 rinsed and dried (8 rinsed, blot dried with adhesive reapplication (9 same as group (6.Then “Tetric Ceram” composite cylinders were bonded to dentin surfaces. Samples were thermo cycled in 5°C and 55°C water, 30 seconds in each bath with a dowel time of 10

  9. Scanning electron microscopic observations of fibrous structure of cemento-dentinal junction in healthy teeth.

    Science.gov (United States)

    Pratebha, B; Jaikumar, N D; Sudhakar, R

    2014-01-01

    The cemento-dentinal junction (CDJ) is a structural and biologic link between cementum and dentin present in the roots of teeth. Conflicting reports about the origin, structure and composition of this layer are present in literature. The width of this junctional tissue is reported to be about 2-4 μm with adhesion of cementum and dentin by proteoglycans and by collagen fiber intermingling. The objective of this study is to observe and report the fibrous architecture of the CDJ of healthy tooth roots. A total of 15 healthy teeth samples were collected, sectioned into halves, demineralized in 5% ethylenediaminetetraacetic acid, processed using NaOH maceration technique and observed under a scanning electron microscope. The CDJ appeared to be a fibril poor groove with a width of 2-4 µm. Few areas of collagen fiber intermingling could be appreciated. A detailed observation of these tissues has been presented.

  10. Mathematical modeling of cell adhesion in shear flow: application to targeted drug delivery in inflammation and cancer metastasis.

    Science.gov (United States)

    Jadhav, Sameer; Eggleton, Charles D; Konstantopoulos, Konstantinos

    2007-01-01

    Cell adhesion plays a pivotal role in diverse biological processes that occur in the dynamic setting of the vasculature, including inflammation and cancer metastasis. Although complex, the naturally occurring processes that have evolved to allow for cell adhesion in the vasculature can be exploited to direct drug carriers to targeted cells and tissues. Fluid (blood) flow influences cell adhesion at the mesoscale by affecting the mechanical response of cell membrane, the intercellular contact area and collisional frequency, and at the nanoscale level by modulating the kinetics and mechanics of receptor-ligand interactions. Consequently, elucidating the molecular and biophysical nature of cell adhesion requires a multidisciplinary approach involving the synthesis of fundamentals from hydrodynamic flow, molecular kinetics and cell mechanics with biochemistry/molecular cell biology. To date, significant advances have been made in the identification and characterization of the critical cell adhesion molecules involved in inflammatory disorders, and, to a lesser degree, in cancer metastasis. Experimental work at the nanoscale level to determine the lifetime, interaction distance and strain responses of adhesion receptor-ligand bonds has been spurred by the advent of atomic force microscopy and biomolecular force probes, although our current knowledge in this area is far from complete. Micropipette aspiration assays along with theoretical frameworks have provided vital information on cell mechanics. Progress in each of the aforementioned research areas is key to the development of mathematical models of cell adhesion that incorporate the appropriate biological, kinetic and mechanical parameters that would lead to reliable qualitative and quantitative predictions. These multiscale mathematical models can be employed to predict optimal drug carrier-cell binding through isolated parameter studies and engineering optimization schemes, which will be essential for developing

  11. The effects of pH on N-methacryloyl glycine primer on bond strength to acid-etched dentin.

    Science.gov (United States)

    Nishiyama, N; Suzuki, K; Asakura, T; Nakai, H; Yasuda, S; Nemoto, K

    1996-07-01

    To develop a more effective adhesive primer, it is imperative to understand the adhesion mechanisms of the resin to the demineralized dentin through a dentin primer. When the bonding agent was directly applied to the dentin etched by 40 wt % phosphoric acid without a primer pretreatment, the bond strength of the resin to the dentin was 5 MPa. Conversely, when the demineralized dentin was pretreated with the N-methacryloyl glycine (NM alpha A) primer solution with a pH value of 1.5, the bond strength increased considerably to 15 MPa. However, the bond strength dropped dramatically from 15 to 3 MPa when the sodium salt of NM alpha A was added, thereby increasing the pH value of the NM alpha A primer solution from 3.2 to 5.0. When the pH value was increased above 3.5 (pKa value), the number of ionized NM alpha A species increased in the solution. As a result, the bond strength of the resin fell to approximately 3 MPa. This result was obtained despite the 5-micron-thick hybrid layer that was created in the subsurface of the intertubular dentin. The number of unionized NM alpha A species increased by lowering the pH value below 3.5. As a result, the NM alpha A primer provided a higher bond strength of the resin to the demineralized dentin. In contrast, when 10 wt % citric acid containing 3 wt % ferric chloride was applied to the dentin, maximum bond strength was obtained when the pH value of the NM alpha A primer solution was 3.5. The pH dependency of the bond strength obtained following 10 wt % citric acid containing 3 wt % ferric chloride etching is different from the results obtained from 40 wt % phosphoric acid etching. This can be attributed to the difference in the characteristics of the demineralized collageous layer.

  12. Analysis on Adhesively-Bonded Joints of FRP-steel Composite Bridge under Combined Loading: Arcan Test Study and Numerical Modeling

    Directory of Open Access Journals (Sweden)

    Xu Jiang

    2016-01-01

    Full Text Available The research presented in this paper is an experimental study and numerical analysis on mechanical behavior of the adhesively-bonded joint between FRP sandwich bridge deck and steel girder. Generally, there are three typical stress states in the adhesively-bonded joint: shear stress, tensile stress, and combination of both. To realize these stress states in the adhesively-bonded joint during tests, a specific loading device is developed with the capacity of providing six different loading angles, which are 0°(pure tension, 18°, 36°, 54°, 72° and 90°(pure shear. Failure modes of adhesively-bonded joints are investigated. It indicates that, for the pure shear loading, the failure mode is the cohesive failure (near the interface between the adhesive layer and the steel support in the adhesive layer. For the pure tensile and combined loading conditions, the failure mode is the combination of fiber breaking, FRP delamination and interfacial adhesion failure between the FRP sandwich deck and the adhesive layer. The load-bearing capacities of adhesive joints under combined loading are much lower than those of the pure tensile and pure shear loading conditions. According to the test results of six angle loading conditions, a tensile/shear failure criterion of the adhesively-bonded joint is obtained. By using Finite Element (FE modeling method, linear elastic simulations are performed to characterize the stress distribution throughout the adhesively-bonded joint.

  13. Hyaluronic acid and oxidized regenerated cellulose prevent adhesion reformation after adhesiolysis in rat models

    OpenAIRE

    Zhang, Yan; Liu, Qin; Yang, Ning; Zhang, Xuegang

    2016-01-01

    Yan Zhang, Qin Liu, Ning Yang, Xuegang Zhang Department of Gynecology, Kunshan Hospital Affiliated to Jiangsu University, Kunshan, Jiangsu, People’s Republic of China Abstract: Postsurgical adhesion formation is the most common complication in abdominal and pelvic surgery. Adhesiolysis is the most commonly applied treatment for adhesion formation but is often followed by adhesion reformation. Therefore, an efficient strategy should be adopted to solve these problems. This study ai...

  14. In vitro evaluation of benzalkonium chloride in the preservation of adhesive interfaces.

    Science.gov (United States)

    Sabatini, C; Kim, J H; Ortiz Alias, P

    2014-01-01

    Inhibition of endogenous dentin matrix metalloproteinases (MMPs) by benzalkonium chloride (BAC) decreases collagen solubilization and may help improve resin-dentin bond stability. This study evaluated the resin-dentin bond stability of experimental adhesive blends containing BAC and the stability of dentin matrices by assessing the mass loss and collagen solubilization from dentin beams pretreated with BAC. Twenty-five healthy molars were used for the bond strength evaluation of a two-step etch-and-rinse adhesive (Adper Single Bond Plus, SB) modified with BAC or not. The following groups were tested: 1) SB with no inhibitor (control); 2) topical 2.0% chlorhexidine + SB; 3) 1.0% BAC etchant + SB; 4) 0.5% BAC-SB; and 5) 1.0% BAC-SB. Microtensile bond strength (μTBS) and failure mode distribution under standard error of the mean were evaluated after 24 hours and six months of storage in artificial saliva (AS). A two-way analysis of variance and Tukey test with a significance level of preduction in dentin bond strength was observed after six months (p<0.05). Less mass loss and HYP release was seen for dentin matrices pretreated with BAC relative to the control pretreated with DW (p<0.05). This in vitro study demonstrates that BAC contributes to the preservation of resin-dentin bonds by reducing collagen degradation.

  15. Comparison of the effects of meloxicam and dexketoprofen on postoperative adhesion formation in a rat uterine horn surgical model.

    Science.gov (United States)

    Keskin, H Levent; Akkus, S Mehmet; Sirin, Y Sinan; Ustuner, Isık; Keles, Hikmet; Ide, Tayfun; Avsar, A Filiz

    2013-01-01

    To compare the effects of 2 nonsteroidal antiinflammatory drugs of different chemical classes (meloxicam and dexketoprofen) on postoperative intraabdominal adhesion formation in a rat model. Experimental study (Canadian Task Force classification I). Center for research and development. Thirty female Wistar albino rats. The animals were randomly assigned to 1 of 3 groups (10 rats per group) and received intramuscular injections of 0.5 mg/kg dexketoprofen (group 1), 0.5 mg/kg meloxicam (group 2), or 1 mL sterile saline solution (control; group 3) daily for 2 days. Laparotomy was performed, and 1 of the uterine horns was damaged via monopolar electrocautery, whereas an incision was made in the other horn using a scalpel and was sutured to promote adhesion formation. The surgeons were blinded to the treatment method. Drug administration was continued for 5 days. The animals were euthanized at 14 days after surgery. Intraperitoneal macroscopic and microscopic adhesions were assessed using standard adhesion scoring systems. Macroscopic adhesion scores were similar among the 3 groups in each horn (p > .50). The total histologic score was significantly lower in the meloxicam group than in the control group (8.0 vs 15.5; p = .006). Dexketoprofen did not significantly affect the total histologic score (11.0 vs 15.5; p = .09) or individual items (i.e., inflammation, fibroblastic activity, foreign body reaction, collagen formation, and vascular proliferation) compared with the control group (p > .02). Meloxicam significantly inhibited inflammation and collagen formation compared with the control group (p dexketoprofen in reducing inflammation (p = .006). Although meloxicam did not affect clinical adhesion formation, it significantly decreased histologic scores compared with those of the control group. Therefore, meloxicam may be suitable in reducing postoperative intraabdominal adhesion formation. Copyright © 2013 AAGL. Published by Elsevier Inc. All rights

  16. Effects of potassium oxalate on knoop hardness of etch-and-rinse adhesives.

    Science.gov (United States)

    Silva, S M A; Malacarne-Zanon, J; Carvalho, R M; Alves, M C; De Goes, M F; Anido-Anido, A; Carrilho, M R

    2012-01-01

    The objective of this study was to determine whether the hardness of etch-and-rinse adhesives may be affected by the pretreatment of acid-etched dentin with potassium oxalate desensitizer. Unerupted human third molars were cut into crown segments by removing the occlusal enamel and roots. The pulp chamber of these crown segments was connected to a syringe barrel filled with phosphate-buffered saline so that the moisture of dentin was maintained during the bonding procedures. Three etch-and-rinse adhesives-two two-step systems (Adper Single Bond 2 [SB], One-Step [OS]) and one three-step system (Adper Scotchbond Multi-Purpose [MP])-were applied to acid-etched dentin that had been treated (experimental groups) or not (control groups) with potassium oxalate (BisBlock). The Knoop hardness (KHN) of adhesives was taken at different sites of the outer surface of the adhesive-bonded dentin. The KHN of the three tested adhesives applied to acid-etched dentin treated with potassium oxalate was significantly lower than that exhibited by the respective controls (not treated with oxalate; padhesive, the treatment with potassium oxalate reduced the adhesives' KHN (psystem exhibiting the lowest KHN compared with the MP and SB systems.

  17. Fluorine analysis of human dentin surrounding resin composite after fluoride application by μ-PIGE/PIXE analysis

    International Nuclear Information System (INIS)

    Okuyama, Katsushi; Komatsu, Hisanori; Yamamoto, Hiroko; Pereira, Patricia N.R.; Bedran-Russo, Ana K.; Nomachi, Masaharu; Sato, Takahiro; Sano, Hidehiko

    2011-01-01

    The use of fluoride for the prevention of caries is based on the transformation of hydroxylapatite to fluoroapatite in the presence of fluoride ions, thereby strengthening tooth structure. Adhesion of dentin and resin composite (tooth-colored restoration material) requires a dentin bonding system, since resin composite is not able to adhere to dentin directly. Demineralization of dentin by acid etching is an important step in the dentin bonding system, however, demineralization also introduces weaknesses in tooth structure. If the demineralized dentin could be strengthened by the application of fluoride, then the dentin-resin composite bond strength might also improve. To test this hypothesis, the present study evaluated the influence of fluoride applications on the strength of the dentin-resin composite bond by (1) tensile strength testing analyses, (2) SEM analyses of tooth structure, and (3) detection of calcium (Ca) and fluorine (F) distribution patterns by micro proton-induced X-ray emission (μ-PIXE) and micro proton-induced gamma-ray emission (μ-PIGE) analyses conducted at the Takasaki Ion Accelerators for Advanced Radiation Application (TIARA) at the Takasaki Advanced Radiation Research Institute (TARRI). In this study, the dentin in extracted human molars was exposed by grinding and the dentin was etched with 35% phosphoric acid. Fluoride was applied at two concentrations, 0.022% (100 ppm F) and 2.21% (10,000 ppm F) NaF solution, for two time periods, 30 and 60 s, prior to bonding the resin composite with the treated dentin. Controls were prepared in the same manner, but without the fluoride application. Bond strength was measured with a micro-tensile testing unit, and the fluorine and calcium distributions at the interface between dentin and resin composite were detected by μ-PIGE and μ-PIXE analysis, respectively. Results indicate that the 10,000 ppm F applications resulted in higher bond strengths than observed in either the 100 ppm F applications or

  18. Fluorine analysis of human dentin surrounding resin composite after fluoride application by μ-PIGE/PIXE analysis

    Science.gov (United States)

    Okuyama, Katsushi; Komatsu, Hisanori; Yamamoto, Hiroko; Pereira, Patricia N. R.; Bedran-Russo, Ana K.; Nomachi, Masaharu; Sato, Takahiro; Sano, Hidehiko

    2011-10-01

    The use of fluoride for the prevention of caries is based on the transformation of hydroxylapatite to fluoroapatite in the presence of fluoride ions, thereby strengthening tooth structure. Adhesion of dentin and resin composite (tooth-colored restoration material) requires a dentin bonding system, since resin composite is not able to adhere to dentin directly. Demineralization of dentin by acid etching is an important step in the dentin bonding system, however, demineralization also introduces weaknesses in tooth structure. If the demineralized dentin could be strengthened by the application of fluoride, then the dentin-resin composite bond strength might also improve. To test this hypothesis, the present study evaluated the influence of fluoride applications on the strength of the dentin-resin composite bond by (1) tensile strength testing analyses, (2) SEM analyses of tooth structure, and (3) detection of calcium (Ca) and fluorine (F) distribution patterns by micro proton-induced X-ray emission (μ-PIXE) and micro proton-induced gamma-ray emission (μ-PIGE) analyses conducted at the Takasaki Ion Accelerators for Advanced Radiation Application (TIARA) at the Takasaki Advanced Radiation Research Institute (TARRI). In this study, the dentin in extracted human molars was exposed by grinding and the dentin was etched with 35% phosphoric acid. Fluoride was applied at two concentrations, 0.022% (100 ppm F) and 2.21% (10,000 ppm F) NaF solution, for two time periods, 30 and 60 s, prior to bonding the resin composite with the treated dentin. Controls were prepared in the same manner, but without the fluoride application. Bond strength was measured with a micro-tensile testing unit, and the fluorine and calcium distributions at the interface between dentin and resin composite were detected by μ-PIGE and μ-PIXE analysis, respectively. Results indicate that the 10,000 ppm F applications resulted in higher bond strengths than observed in either the 100 ppm F applications or

  19. Fluorine analysis of human dentin surrounding resin composite after fluoride application by {mu}-PIGE/PIXE analysis

    Energy Technology Data Exchange (ETDEWEB)

    Okuyama, Katsushi, E-mail: katsu@den.hokudai.ac.jp [Graduate School of Dental Medicine, Hokkaido University, Department of Restorative Dentistry, Kita-13, Nishi-7, Kita-ku, Hokkaido, Sapporo 060-8586 (Japan) and School of Dentistry, University of North Carolina, Department of Operative Dentistry, 302 Brauer, CB 7450, Chapel Hill, NC 27599-7450 (United States); Komatsu, Hisanori [Graduate School of Dental Medicine, Hokkaido University, Department of Restorative Dentistry, Kita-13, Nishi-7, Kita-ku, Hokkaido, Sapporo 060-8586 (Japan); Yamamoto, Hiroko [Graduate School of Dentistry, Osaka University, 1-8 Yamada-Oka, Osaka, Suita 565-0871 (Japan); Pereira, Patricia N.R. [School of Dentistry, University of North Carolina, Department of Operative Dentistry, 302 Brauer, CB 7450, Chapel Hill, NC 27599-7450 (United States); Bedran-Russo, Ana K. [University of Illinois at Chicago, College of Dentistry, Department of Restorative Dentistry, 801 S. Paulina St., Chicago, IL 60612 (United States); Nomachi, Masaharu [Graduate School of Science, Osaka University, 1-1 Machikaneyama, Osaka, Toyonaka 560-0043 (Japan); Sato, Takahiro [TARRI, JAEA, Advanced Radiation Technology, 1233 Watanuki-machi, Gunma, Takasaki 370-1292 (Japan); Sano, Hidehiko [Graduate School of Dental Medicine, Hokkaido University, Department of Restorative Dentistry, Kita-13, Nishi-7, Kita-ku, Hokkaido, Sapporo 060-8586 (Japan)

    2011-10-15

    The use of fluoride for the prevention of caries is based on the transformation of hydroxylapatite to fluoroapatite in the presence of fluoride ions, thereby strengthening tooth structure. Adhesion of dentin and resin composite (tooth-colored restoration material) requires a dentin bonding system, since resin composite is not able to adhere to dentin directly. Demineralization of dentin by acid etching is an important step in the dentin bonding system, however, demineralization also introduces weaknesses in tooth structure. If the demineralized dentin could be strengthened by the application of fluoride, then the dentin-resin composite bond strength might also improve. To test this hypothesis, the present study evaluated the influence of fluoride applications on the strength of the dentin-resin composite bond by (1) tensile strength testing analyses, (2) SEM analyses of tooth structure, and (3) detection of calcium (Ca) and fluorine (F) distribution patterns by micro proton-induced X-ray emission ({mu}-PIXE) and micro proton-induced gamma-ray emission ({mu}-PIGE) analyses conducted at the Takasaki Ion Accelerators for Advanced Radiation Application (TIARA) at the Takasaki Advanced Radiation Research Institute (TARRI). In this study, the dentin in extracted human molars was exposed by grinding and the dentin was etched with 35% phosphoric acid. Fluoride was applied at two concentrations, 0.022% (100 ppm F) and 2.21% (10,000 ppm F) NaF solution, for two time periods, 30 and 60 s, prior to bonding the resin composite with the treated dentin. Controls were prepared in the same manner, but without the fluoride application. Bond strength was measured with a micro-tensile testing unit, and the fluorine and calcium distributions at the interface between dentin and resin composite were detected by {mu}-PIGE and {mu}-PIXE analysis, respectively. Results indicate that the 10,000 ppm F applications resulted in higher bond strengths than observed in either the 100 ppm F

  20. Effect of composite resin placement techniques on the microleakage of two self-etching dentin-bonding agents.

    NARCIS (Netherlands)

    Santini, A.; Plasschaert, A.J.M.; Mitchell, S.M.

    2001-01-01

    PURPOSE: To evaluate microleakage of Class V resin-based composites (RBC) bonded with two self-etching dentin adhesive systems. Class V cavities were restored with either one or three increments of RBC to determine whether the restorative method affected microleakage. MATERIALS AND METHODS: 60

  1. Application of 10% Ascorbic Acid Improves Resin Shear Bond Stregth in Bleached Dentin

    Directory of Open Access Journals (Sweden)

    Kamizar Kamizar

    2014-10-01

    Full Text Available Normal 0 false false false IN X-NONE X-NONE MicrosoftInternetExplorer4 Restoration of the teeth immediately after bleaching with H2O2 35% is contraindicated due to the remnants of free radical that will stay inside dentin for 2-3 weeks which will compromise the adhesiveness of composite resin. Objective: The aim of this study was to evaluate the influence of 10% ascorbic acid on shear bond strength of composite placed on bleached dentin. Methods:Twenty seven samples were divided equally into three groups. Group 1: dentin was etched with 35% phosphoric acid; Group 2: dentin was bleached with 35% H2O2 followed by etching with 35% phosphoric acid; Group 3: dentin was bleached with 35% H2O2, followed by application of 10% ascorbic acid and etched with 35% phosphoric acid. All samples were then stored at 370C for 24 hours. The Universal Testing Machine was used to measure shear bond strength and the results were analyzed with Kruskal Wallis and Mann Whitney test. Results: After nine independent experiments, 10% ascorbic acid application on bleached dentin resulted in highest increased in bond stregth (56.04±11.06MPa compared to Group 2 (29.09±7.63MPa and Group 1 (25.55±2.22MPa and the difference was statistically significant (p<0.05. Conclusion: Application of 10% ascorbic acid to the bleached dentin improved the shear bond strength of resin composite.

  2. Sodium Hypochlorite Irrigation and Its Effect on Bond Strength to Dentin

    Directory of Open Access Journals (Sweden)

    Tariq S. Abuhaimed

    2017-01-01

    Full Text Available Effective shaping and cleaning of root canals are essential for the success of endodontic treatment. Due to the complex anatomy of root canal spaces, the use of various instrumentation techniques alone is not effective in producing bacteria-free root canal spaces. Irrigation, disinfectants, rinses, and intervisit medications are used in conjunction with the mechanical instrumentation to ensure the success of endodontic treatment. Sodium hypochlorite (NaOCl, a halogenated compound, is routinely used to irrigate the root canal during endodontic treatments. NaOCl has been known for its antibacterial action, proteolytic and dissolution capacity, and debridement properties. NaOCl, however, can alter the composition of dentin and hence its interaction with the adhesive resins used to bond the restorative materials to treated dentin. This review therefore covers in depth the action of NaOCl on dentin-adhesive resin bond strength including both enhancement and reduction, then mechanisms proposed for such action, and finally how the adverse action of NaOCl on dentin can be reversed.

  3. Sodium Hypochlorite Irrigation and Its Effect on Bond Strength to Dentin.

    Science.gov (United States)

    Abuhaimed, Tariq S; Abou Neel, Ensanya A

    2017-01-01

    Effective shaping and cleaning of root canals are essential for the success of endodontic treatment. Due to the complex anatomy of root canal spaces, the use of various instrumentation techniques alone is not effective in producing bacteria-free root canal spaces. Irrigation, disinfectants, rinses, and intervisit medications are used in conjunction with the mechanical instrumentation to ensure the success of endodontic treatment. Sodium hypochlorite (NaOCl), a halogenated compound, is routinely used to irrigate the root canal during endodontic treatments. NaOCl has been known for its antibacterial action, proteolytic and dissolution capacity, and debridement properties. NaOCl, however, can alter the composition of dentin and hence its interaction with the adhesive resins used to bond the restorative materials to treated dentin. This review therefore covers in depth the action of NaOCl on dentin-adhesive resin bond strength including both enhancement and reduction, then mechanisms proposed for such action, and finally how the adverse action of NaOCl on dentin can be reversed.

  4. Assessing potential peptide targeting ligands by quantification of cellular adhesion of model nanoparticles under flow conditions.

    Science.gov (United States)

    Broda, Ellen; Mickler, Frauke Martina; Lächelt, Ulrich; Morys, Stephan; Wagner, Ernst; Bräuchle, Christoph

    2015-09-10

    Sophisticated drug delivery systems are coated with targeting ligands to improve the specific adhesion to surface receptors on diseased cells. In our study, we developed a method with which we assessed the potential of peptide ligands to specifically bind to receptor overexpressing target cells. Therefore, a microfluidic setup was used where the cellular adhesion of nanoparticles with ligand and of control nanoparticles was observed in parallel under the same experimental conditions. The effect of the ligand on cellular binding was quantified by counting the number of adhered nanoparticles with ligand and differently labeled control nanoparticles on single cells after incubation under flow conditions. To provide easy-to-synthesize, stable and reproducible nanoparticles which mimic the surface characteristics of drug delivery systems and meet the requirements for quantitative analysis, latex beads based on amine-modified polystyrene were used as model nanoparticles. Two short peptides were tested to serve as targeting ligand on the beads by increasing the specific binding to HuH7 cells. The c-Met binding peptide cMBP2 was used for hepatocyte growth factor receptor (c-Met) targeting and the peptide B6 for transferrin receptor (TfR) targeting. The impact of the targeting peptide on binding was investigated by comparing the beads with ligand to different internal control beads: 1) without ligand and tailored surface charge (electrostatic control) and 2) with scrambled peptide and similar surface charge, but a different amino acid sequence (specificity control). Our results demonstrate that the method is very useful to select suitable targeting ligands for specific nanoparticle binding to receptor overexpressing tumor cells. We show that the cMBP2 ligand specifically enhances nanoparticle adhesion to target cells, whereas the B6 peptide mediates binding to tumor cells mainly by nonspecific interactions. All together, we suggest that cMBP2 is a suitable choice for

  5. Dry adhesives with sensing features

    International Nuclear Information System (INIS)

    Krahn, J; Menon, C

    2013-01-01

    Geckos are capable of detecting detachment of their feet. Inspired by this basic observation, a novel functional dry adhesive is proposed, which can be used to measure the instantaneous forces and torques acting on an adhesive pad. Such a novel sensing dry adhesive could potentially be used by climbing robots to quickly realize and respond appropriately to catastrophic detachment conditions. The proposed torque and force sensing dry adhesive was fabricated by mixing Carbon Black (CB) and Polydimethylsiloxane (PDMS) to form a functionalized adhesive with mushroom caps. The addition of CB to PDMS resulted in conductive PDMS which, when under compression, tension or torque, resulted in a change in the resistance across the adhesive patch terminals. The proposed design of the functionalized dry adhesive enables distinguishing an applied torque from a compressive force in a single adhesive pad. A model based on beam theory was used to predict the change in resistance across the terminals as either a torque or compressive force was applied to the adhesive patch. Under a compressive force, the sensing dry adhesive was capable of measuring compression stresses from 0.11 Pa to 20.9 kPa. The torque measured by the adhesive patch ranged from 2.6 to 10 mN m, at which point the dry adhesives became detached. The adhesive strength was 1.75 kPa under an applied preload of 1.65 kPa for an adhesive patch with an adhesive contact area of 7.07 cm 2 . (paper)

  6. Dry adhesives with sensing features

    Science.gov (United States)

    Krahn, J.; Menon, C.

    2013-08-01

    Geckos are capable of detecting detachment of their feet. Inspired by this basic observation, a novel functional dry adhesive is proposed, which can be used to measure the instantaneous forces and torques acting on an adhesive pad. Such a novel sensing dry adhesive could potentially be used by climbing robots to quickly realize and respond appropriately to catastrophic detachment conditions. The proposed torque and force sensing dry adhesive was fabricated by mixing Carbon Black (CB) and Polydimethylsiloxane (PDMS) to form a functionalized adhesive with mushroom caps. The addition of CB to PDMS resulted in conductive PDMS which, when under compression, tension or torque, resulted in a change in the resistance across the adhesive patch terminals. The proposed design of the functionalized dry adhesive enables distinguishing an applied torque from a compressive force in a single adhesive pad. A model based on beam theory was used to predict the change in resistance across the terminals as either a torque or compressive force was applied to the adhesive patch. Under a compressive force, the sensing dry adhesive was capable of measuring compression stresses from 0.11 Pa to 20.9 kPa. The torque measured by the adhesive patch ranged from 2.6 to 10 mN m, at which point the dry adhesives became detached. The adhesive strength was 1.75 kPa under an applied preload of 1.65 kPa for an adhesive patch with an adhesive contact area of 7.07 cm2.

  7. Hereditary dentine disorders: dentinogenesis imperfecta and dentine dysplasia

    Directory of Open Access Journals (Sweden)

    MacKie Iain

    2008-11-01

    Full Text Available Abstract The hereditary dentine disorders, dentinogenesis imperfecta (DGI and dentine dysplasia (DD, comprise a group of autosomal dominant genetic conditions characterised by abnormal dentine structure affecting either the primary or both the primary and secondary dentitions. DGI is reported to have an incidence of 1 in 6,000 to 1 in 8,000, whereas that of DD type 1 is 1 in 100,000. Clinically, the teeth are discoloured and show structural defects such as bulbous crowns and small pulp chambers radiographically. The underlying defect of mineralisation often results in shearing of the overlying enamel leaving exposed weakened dentine which is prone to wear. Currently, three sub-types of DGI and two sub-types of DD are recognised but this categorisation may change when other causative mutations are found. DGI type I is inherited with osteogenesis imperfecta and recent genetic studies have shown that mutations in the genes encoding collagen type 1, COL1A1 and COL1A2, underlie this condition. All other forms of DGI and DD, except DD-1, appear to result from mutations in the gene encoding dentine sialophosphoprotein (DSPP, suggesting that these conditions are allelic. Diagnosis is based on family history, pedigree construction and detailed clinical examination, while genetic diagnosis may become useful in the future once sufficient disease-causing mutations have been discovered. Differential diagnoses include hypocalcified forms of amelogenesis imperfecta, congenital erythropoietic porphyria, conditions leading to early tooth loss (Kostmann's disease, cyclic neutropenia, Chediak-Hegashi syndrome, histiocytosis X, Papillon-Lefevre syndrome, permanent teeth discolouration due to tetracyclines, Vitamin D-dependent and vitamin D-resistant rickets. Treatment involves removal of sources of infection or pain, improvement of aesthetics and protection of the posterior teeth from wear. Beginning in infancy, treatment usually continues into adulthood with a

  8. Influence of application methods of one-step self-etching adhesives on microtensile bond strength

    OpenAIRE

    Chul-Kyu Choi,; Sung-Ae Son; Jin-Hee Ha; Bock Hur; Hyeon-Cheol Kim; Yong-Hun Kwon; Jeong-Kil Park

    2011-01-01

    Objectives The purpose of this study was to evaluate the effect of various application methods of one-step self-etch adhesives to microtensile resin-dentin bond strength. Materials and Methods Thirty-six extracted human molars were used. The teeth were assigned randomly to twelve groups (n = 15), according to the three different adhesive systems (Clearfil Tri-S Bond, Adper Prompt L-Pop, G-Bond) and application methods. The adhesive systems were applied on the dentin as follows: 1) T...

  9. Effect of universal adhesive etching modes on bond strength to dual-polymerizing composite resins.

    Science.gov (United States)

    Michaud, Pierre-Luc; Brown, Matthew

    2018-04-01

    Information is lacking as to the effect on bond strength of the etching modes of universal adhesives when they are used to bond dual-polymerizing composite resins to dentin. The purpose of this in vitro study was to investigate the bonding of dual-polymerizing foundation composite resins to dentin when universal bonding agents are used in self-etch or etch-and-rinse modes. Sixty caries-free, extracted third molar teeth were sectioned transversely in the apical third of the crown and allocated to 12 groups (n=5). Three different bonding agents (Scotchbond Universal, OptiBond XTR, All-Bond Universal) were used to bond 2 different dual-polymerizing composite resins (CompCore AF or CoreFlo DC) to dentin, using 2 different etching approaches (etch-and-rinse or self-etch). The specimens were sectioned into sticks (1×1×8 mm) with a precision saw. The bond strength of the specimens was tested under microtensile force at a crosshead speed of 0.5 mm/min. The data were analyzed using a 3-way ANOVA, a Games-Howell post hoc comparisons model, and Student t tests with Bonferroni corrections (α=.05). In the overall model, the composite resin used had no effect on bond strength (P=.830). The etching protocol by itself also did not have a significant effect (P=.059), although a trend was present. The bonding agent, however, did have an effect (Pcomposite resins to dentin, no single etching protocol is better than another. Depending on which bonding agent is being used, one etching mode may perform better. Copyright © 2017 Editorial Council for the Journal of Prosthetic Dentistry. Published by Elsevier Inc. All rights reserved.

  10. Histatin 1 Enhances Cell Adhesion to Titanium in an Implant Integration Model.

    Science.gov (United States)

    van Dijk, I A; Beker, A F; Jellema, W; Nazmi, K; Wu, G; Wismeijer, D; Krawczyk, P M; Bolscher, J G M; Veerman, E C I; Stap, J

    2017-04-01

    Cellular adhesion is essential for successful integration of dental implants. Rapid soft tissue integration is important to create a seal around the implant and prevent infections, which commonly cause implant failure and can result in bone loss. In addition, soft tissue management is important to obtain good dental aesthetics. We previously demonstrated that the salivary peptide histatin 1 (Hst1) causes a more than 2-fold increase in the ability of human adherent cells to attach and spread on a glass surface. Cells treated with Hst1 attached more rapidly and firmly to the substrate and to each other. In the current study, we examine the potential application of Hst1 for promotion of dental implant integration. Our results show that Hst1 enhances the attachment and spreading of soft tissue cell types (oral epithelial cells and fibroblasts) to titanium (Ti) and hydroxyapatite (HAP), biomaterials that have found wide applications as implant material in dentistry and orthopedics. For improved visualization of cell adhesion to Ti, we developed a novel technique that uses sputtering to deposit a thin, transparent layer of Ti onto glass slides. This approach allows detailed, high-resolution analysis of cell adherence to Ti in real time. Furthermore, our results suggest that Hst1 has no negative effects on cell survival. Given its natural occurrence in the oral cavity, Hst1 could be an attractive agent for clinical application. Importantly, even though Hst1 is specific for saliva of humans and higher primates, it stimulated the attachment and spreading of canine cells, paving the way for preclinical studies in canine models.

  11. Protein adhesives

    Science.gov (United States)

    Charles R. Frihart; Linda F. Lorenz

    2018-01-01

    Nature uses a wide variety of chemicals for providing adhesion internally (e.g., cell to cell) and externally (e.g., mussels to ships and piers). This adhesive bonding is chemically and mechanically complex, involving a variety of proteins, carbohydrates, and other compounds.Consequently,the effect of protein structures on adhesive properties is only partially...

  12. Microshear bond strength of preheated silorane- and methacrylate-based composite resins to dentin.

    Science.gov (United States)

    Demirbuga, Sezer; Ucar, Faruk Izzet; Cayabatmaz, Muhammed; Zorba, Yahya Orcun; Cantekin, Kenan; Topçuoğlu, Hüseyin Sinan; Kilinc, Halil Ibrahim

    2016-01-01

    The aim of this study was to investigate the effect of preheating on microshear bond strength (MSBS) of silorane and methacrylate-based composite resins to human dentin. The teeth were randomly divided into three main groups: (1) composite resins were heated upto 68 °C; (2) cooled to 4 °C; and (3) control [room temperature (RT)]. Each group was then randomly subdivided into four subgroups according to adhesive system used [Solobond M (Voco), All Bond SE (Bisco), Clearfil SE Bond (CSE) (Kuraray), Silorane adhesive system (SAS) (3M ESPE)]. Resin composite cylinders were formed (0.9 mm diameter × 0.7 mm length) and MSBS of each specimen was tested. The preheated groups exhibited the highest MSBS (p composite resins may be an alternative way to increase the MSBS of composites on dentin. © Wiley Periodicals, Inc.

  13. Cyclophilin B Deficiency Causes Abnormal Dentin Collagen Matrix.

    Science.gov (United States)

    Terajima, Masahiko; Taga, Yuki; Cabral, Wayne A; Nagasawa, Masako; Sumida, Noriko; Hattori, Shunji; Marini, Joan C; Yamauchi, Mitsuo

    2017-08-04

    Cyclophilin B (CypB) is an endoplasmic reticulum-resident protein that regulates collagen folding, and also contributes to prolyl 3-hydroxylation (P3H) and lysine (Lys) hydroxylation of collagen. In this study, we characterized dentin type I collagen in CypB null (KO) mice, a model of recessive osteogenesis imperfecta type IX, and compared to those of wild-type (WT) and heterozygous (Het) mice. Mass spectrometric analysis demonstrated that the extent of P3H in KO collagen was significantly diminished compared to WT/Het. Lys hydroxylation in KO was significantly diminished at the helical cross-linking sites, α1/α2(I) Lys-87 and α1(I) Lys-930, leading to a significant increase in the under-hydroxylated cross-links and a decrease in fully hydroxylated cross-links. The extent of glycosylation of hydroxylysine residues was, except α1(I) Lys-87, generally higher in KO than WT/Het. Some of these molecular phenotypes were distinct from other KO tissues reported previously, indicating the dentin-specific control mechanism through CypB. Histological analysis revealed that the width of predentin was greater and irregular, and collagen fibrils were sparse and significantly smaller in KO than WT/Het. These results indicate a critical role of CypB in dentin matrix formation, suggesting a possible association between recessive osteogenesis imperfecta and dentin defects that have not been clinically detected.

  14. Evaluation of the bond strength of resin cements used to lute ceramics on laser-etched dentin.

    Science.gov (United States)

    Giray, Figen Eren; Duzdar, Lale; Oksuz, Mustafa; Tanboga, Ilknur

    2014-07-01

    The purpose of this study was to investigate the shear bond strength (SBS) of two different adhesive resin cements used to lute ceramics on laser-etched dentin. Erbium, chromium: yttrium, scandium, gallium, garnet (Er,Cr:YSGG) laser irradiation has been claimed to improve the adhesive properties of dentin, but results to date have been controversial, and its compatibility with existing adhesive resin cements has not been conclusively determined. Two adhesive cements, one "etch-and-rinse" [Variolink II (V)] and one "self-etch" [Clearfil Esthetic Cement (C)] luting cement, were used to lute ceramic blocks (Vita Celay Blanks, Vita) onto dentin surfaces. In total, 80 dentin specimens were distributed randomly into eight experimental groups according to the dentin surface-etching technique used Er,Cr:YSGG laser and Er:YAG laser: (1) 37% orthophosphoric acid+V (control group), (2) Er,Cr:YSGG laser+V, (3) Er,Cr:YSGG laser+acid+V, (4) Er:YAG laser+V, (5) Er:YAG laser+acid+V, (6) C, (7) Er,Cr:YSGG laser+C, and (8) Er:YAG laser+C. Following these applications, the ceramic discs were bonded to prepared surfaces and were shear loaded in a universal testing machine until fracture. SBS was recorded for each group in MPa. Shear test values were evaluated statistically using the Mann-Whitney U test. No statistically significant differences were evident between the control group and the other groups (p>0.05). The Er,Cr:YSGG laser+A+V group demonstrated significantly higher SBS than did the Er,Cr:YSGG laser+V group (p=0.034). The Er,Cr:YSGG laser+C and Er:YAG laser+C groups demonstrated significantly lower SBS than did the C group (pceramic bond strengths, depending upon the adhesive cement used.

  15. A space-jump derivation for non-local models of cell-cell adhesion and non-local chemotaxis.

    Science.gov (United States)

    Buttenschön, Andreas; Hillen, Thomas; Gerisch, Alf; Painter, Kevin J

    2018-01-01

    Cellular adhesion provides one of the fundamental forms of biological interaction between cells and their surroundings, yet the continuum modelling of cellular adhesion has remained mathematically challenging. In 2006, Armstrong et al. proposed a mathematical model in the form of an integro-partial differential equation. Although successful in applications, a derivation from an underlying stochastic random walk has remained elusive. In this work we develop a framework by which non-local models can be derived from a space-jump process. We show how the notions of motility and a cell polarization vector can be naturally included. With this derivation we are able to include microscopic biological properties into the model. We show that particular choices yield the original Armstrong model, while others lead to more general models, including a doubly non-local adhesion model and non-local chemotaxis models. Finally, we use random walk simulations to confirm that the corresponding continuum model represents the mean field behaviour of the stochastic random walk.

  16. Reconstructing the life-time lead exposure in children using dentine in deciduous teeth

    Energy Technology Data Exchange (ETDEWEB)

    Shepherd, Thomas J., E-mail: shepherdtj@aol.com [School of Earth and Environment, University of Leeds, Leeds LS2 9JT (United Kingdom); Dirks, Wendy [Centre for Oral Health Research, School of Dental Sciences, Newcastle University, Newcastle upon Tyne NE2 4BW (United Kingdom); Manmee, Charuwan; Hodgson, Susan [Institute of Health and Society, Newcastle University, Newcastle upon Tyne NE2 4AX (United Kingdom); Banks, David A. [School of Earth and Environment, University of Leeds, Leeds LS2 9JT (United Kingdom); Averley, Paul [Centre for Oral Health Research, School of Dental Sciences, Newcastle University, Newcastle upon Tyne NE2 4BW (United Kingdom); Queensway Dental Practice, 170 Queensway, Billingham, Teesside TS23 2NT (United Kingdom); Pless-Mulloli, Tanja [Institute of Health and Society, Newcastle University, Newcastle upon Tyne NE2 4AX (United Kingdom); Newcastle Institute for Research on Sustainability, Newcastle University, Newcastle upon Tyne NE1 7RU (United Kingdom)

    2012-05-15

    Data are presented to demonstrate that the circumpulpal dentine of deciduous teeth can be used to reconstruct a detailed record of childhood exposure to lead. By combining high spatial resolution laser ablation ICP-MS with dental histology, information was acquired on the concentration of lead in dentine from in utero to several years after birth, using a true time template of dentine growth. Time corrected lead analyses for pairs of deciduous molars confirmed that between-tooth variation for the same child was negligible and that meaningful exposure histories can be obtained from a single, multi-point ablation transect on longitudinal sections of individual teeth. For a laser beam of 100 {mu}m diameter, the lead signal for each ablation point represented a time span of 42 days. Simultaneous analyses for Sr, Zn and Mg suggest that the incorporation of Pb into dentine (carbonated apatite) is most likely controlled by nanocrystal growth mechanisms. The study also highlights the importance of discriminating between primary and secondary dentine and the dangers of translating lead analyses into blood lead estimates without determining the age or duration of dentine sampled. Further work is in progress to validate deciduous teeth as blood lead biomarkers. - Highlights: Black-Right-Pointing-Pointer Reconstruction of childhood exposure history to Pb using deciduous tooth dentine. Black-Right-Pointing-Pointer Pb analyses acquired for dentine growth increments of 42 days. Black-Right-Pointing-Pointer Highly correlated Pb concentration profiles for pairs of deciduous molars. Black-Right-Pointing-Pointer Data for Sr, Zn and Mg provide a model for the incorporation of Pb into dentine.

  17. Reconstructing the life-time lead exposure in children using dentine in deciduous teeth

    International Nuclear Information System (INIS)

    Shepherd, Thomas J.; Dirks, Wendy; Manmee, Charuwan; Hodgson, Susan; Banks, David A.; Averley, Paul; Pless-Mulloli, Tanja

    2012-01-01

    Data are presented to demonstrate that the circumpulpal dentine of deciduous teeth can be used to reconstruct a detailed record of childhood exposure to lead. By combining high spatial resolution laser ablation ICP-MS with dental histology, information was acquired on the concentration of lead in dentine from in utero to several years after birth, using a true time template of dentine growth. Time corrected lead analyses for pairs of deciduous molars confirmed that between-tooth variation for the same child was negligible and that meaningful exposure histories can be obtained from a single, multi-point ablation transect on longitudinal sections of individual teeth. For a laser beam of 100 μm diameter, the lead signal for each ablation point represented a time span of 42 days. Simultaneous analyses for Sr, Zn and Mg suggest that the incorporation of Pb into dentine (carbonated apatite) is most likely controlled by nanocrystal growth mechanisms. The study also highlights the importance of discriminating between primary and secondary dentine and the dangers of translating lead analyses into blood lead estimates without determining the age or duration of dentine sampled. Further work is in progress to validate deciduous teeth as blood lead biomarkers. - Highlights: ► Reconstruction of childhood exposure history to Pb using deciduous tooth dentine. ► Pb analyses acquired for dentine growth increments of 42 days. ► Highly correlated Pb concentration profiles for pairs of deciduous molars. ► Data for Sr, Zn and Mg provide a model for the incorporation of Pb into dentine.

  18. The comparison between two irrigation regimens on the dentine wettability for an epoxy resin based sealer by measuring its contact angle formed to the irrigated dentine.

    Science.gov (United States)

    Mohan, Rayapudi Phani; Pai, Annappa Raghavendra Vivekananda

    2015-01-01

    The aim was to assess the influence of two irrigation regimens having ethylenediaminetetraacetic acid (EDTA) and ethylenediaminetetraacetic acid with cetrimide (EDTAC) as final irrigants, respectively, on the dentine wettability for AH Plus sealer by comparing its contact angle formed to the irrigated dentine. Study samples were divided into two groups (n = 10). The groups were irrigated with 3% sodium hypochlorite (NaOCl) solution followed by either 17% EDTA or 17% EDTAC solution. AH Plus was mixed, and controlled volume droplet (0.1 mL) of the sealer was placed on the dried samples. The contact angle was measured using a Dynamic Contact Angle Analyzer and results were analyzed using SPSS 21.0 and 2 sample t-test. There was a significant difference in the contact angle of AH Plus formed to the dentine irrigated with the above two regimens. AH Plus showed significantly lower contact angle with the regimen having EDTAC as a final irrigant than the one with EDTA (P contact angle of a sealer. EDTAC as a final irrigant facilitates better dentin wettability than EDTA for AH Plus to promote its better flow and adhesion.

  19. Modeling and design optimization of adhesion between surfaces at the microscale.

    Energy Technology Data Exchange (ETDEWEB)

    Sylves, Kevin T. (University of Colorado, Boulder, CO)

    2008-08-01

    This research applies design optimization techniques to structures in adhesive contact where the dominant adhesive mechanism is the van der Waals force. Interface finite elements are developed for domains discretized by beam elements, quadrilateral elements or triangular shell elements. Example analysis problems comparing finite element results to analytical solutions are presented. These examples are then optimized, where the objective is matching a force-displacement relationship and the optimization variables are the interface element energy of adhesion or the width of beam elements in the structure. Several parameter studies are conducted and discussed.

  20. Cell adhesion and spreading at a charged interface: Insight into the mechanism using surface techniques and mathematical modelling

    International Nuclear Information System (INIS)

    DeNardis, Nadica Ivošević; Ilić, Jadranka Pečar; Ružić, Ivica; Pletikapić, Galja

    2015-01-01

    Highlights: • Kinetics of adhesion and spreading of the algal cell at a charged interface is explored. • Amperometric signals are analyzed using extended methodology and the reaction kinetics model. • The model reconstructs and quantifies individual states of the three-step adhesion process. • Adhesion kinetics of the algal cell is slower than that of its plasma membrane vesicle. • Slow spreading of organic film at the interface could be due to the attenuated effect of the potential. - Abstract: We study the kinetics of adhesion and spreading of an algal cell and its plasma membrane vesicle at the charged interface. A simple system of an isolated plasma membrane vesicle without internal content has been developed and characterized by atomic force microscopy (AFM). We extend the methodology based on the reaction kinetics model and empirical fitting for the analysis of amperometric signals, and demonstrate its validity and pertinence in a wide range of surface charge densities. Adhesion kinetics of the algal cell is slower than that of its plasma membrane vesicle. Isolated plasma membrane contributes about one quarter to the cell contact area. The model reconstructs and quantifies individual states of the three-step adhesion process of the algal cell and makes it possible to associate them with various features of amperometric signal. At the time of current amplitude, the ruptured state predominates and the cell spread contact area is larger than its initial area as well as the contact area of the plasma membrane vesicle. These results suggest that a major structural disruption of the cell membrane, collapse of cytoskeleton and leakage of intracellular material could appear close to the time of current amplitude. Further, slow kinetics of the organic film spreading at the interface to its maximal extent is considered as the rate determining step, which could be a consequence of the attenuated effect of potential at the modified interface, stronger

  1. The effect of the physical properties of the substrate on the kinetics of cell adhesion and crawling studied by an axisymmetric diffusion-energy balance coupled model.

    Science.gov (United States)

    Samadi-Dooki, Aref; Shodja, Hossein M; Malekmotiei, Leila

    2015-05-14

    In this paper an analytical approach to study the effect of the substrate physical properties on the kinetics of adhesion and motility behavior of cells is presented. Cell adhesion is mediated by the binding of cell wall receptors and substrate's complementary ligands, and tight adhesion is accomplished by the recruitment of the cell wall binders to the adhesion zone. The binders' movement is modeled as their axisymmetric diffusion in the fluid-like cell membrane. In order to preserve the thermodynamic consistency, the energy balance for the cell-substrate interaction is imposed on the diffusion equation. Solving the axisymmetric diffusion-energy balance coupled equations, it turns out that the physical properties of the substrate (substrate's ligand spacing and stiffness) have considerable effects on the cell adhesion and motility kinetics. For a rigid substrate with uniform distribution of immobile ligands, the maximum ligand spacing which does not interrupt adhesion growth is found to be about 57 nm. It is also found that as a consequence of the reduction in the energy dissipation in the isolated adhesion system, cell adhesion is facilitated by increasing substrate's stiffness. Moreover, the directional movement of cells on a substrate with gradients in mechanical compliance is explored with an extension of the adhesion formulation. It is shown that cells tend to move from soft to stiff regions of the substrate, but their movement is decelerated as the stiffness of the substrate increases. These findings based on the proposed theoretical model are in excellent agreement with the previous experimental observations.

  2. Effect of moisture and drying time on the bond strength of the one-step self-etching adhesive system

    Directory of Open Access Journals (Sweden)

    Yoon Lee

    2012-08-01

    Full Text Available Objectives To investigate the effect of dentin moisture degree and air-drying time on dentin-bond strength of two different one-step self-etching adhesive systems. Materials and Methods Twenty-four human third molars were used for microtensile bond strength testing of G-Bond and Clearfil S3 Bond. The dentin surface was either blot-dried or air-dried before applying these adhesive agents. After application of the adhesive agent, three different air drying times were evaluated: 1, 5, and 10 sec. Composite resin was build up to 4 mm thickness and light cured for 40 sec with 2 separate layers. Then the tooth was sectioned and trimmed to measure the microtensile bond strength using a universal testing machine. The measured bond strengths were analyzed with three-way ANOVA and regression analysis was done (p = 0.05. Results All three factors, materials, dentin wetness and air drying time, showed significant effect on the microtensile bond strength. Clearfil S3 Bond, dry dentin surface and 10 sec air drying time showed higher bond strength. Conclusions Within the limitation of this experiment, air drying time after the application of the one-step self-etching adhesive agent was the most significant factor affecting the bond strength, followed by the material difference and dentin moisture before applying the adhesive agent.

  3. Unicystic ameloblastoma with the presence of dentin

    Directory of Open Access Journals (Sweden)

    Sivapathasundharam B

    2007-01-01

    Full Text Available We present a case of unicystic ameloblastoma of the posterior mandible in a 28 year-old female, histologically showing luminal and intramural plexiform epithelial proliferation with typical dentin in the connective tissue capsule. The characteristics of hard tissue formation in ameloblastomas reported in existing literature and the possible origin of the dentin mass seen in our case are discussed.

  4. Raman spectra of human dentin mineral

    NARCIS (Netherlands)

    Tsuda, H; Ruben, J; Arends, J

    Human dentin mineral has been investigated by using micro-Raman spectroscopy. Fluorescence and thermal problems were largely avoided by preparing dentin samples by grinding and ultrasonic agitation in acetone. The Raman spectral features were consistent with those of impure hydroxyapatite containing

  5. Effect of hyaluronic acid on postoperative intraperitoneal adhesion formation in the rat model

    Energy Technology Data Exchange (ETDEWEB)

    Urman, B.; Gomel, V.; Jetha, N. (Department of Obstetrics and Gynecology, University of British Columbia, Vancouver (Canada))

    1991-09-01

    The aim of this study was to determine the effectiveness of hyaluronic acid solution in preventing intraperitoneal (IP) adhesions. The study design was prospective, randomized and blinded and involved 83 rats. Measured serosal injury was inflicted using a CO2 laser on the right uterine horn of the rat. Animals randomized to groups 1 and 2 received either 0.4% hyaluronic acid or its diluent phosphate-buffered saline (PBS) intraperitoneally before and after the injury. In groups 3 and 4, the same solutions were used only after the injury. Postoperative adhesions were assessed at second-look laparotomy. Histologic assessment of the fresh laser injury was carried out on uteri pretreated with hyaluronic acid, PBS, or nothing. Pretreatment with hyaluronic acid was associated with a significant reduction in postoperative adhesions and a significantly decreased crater depth. Hyaluronic acid appears to reduce postoperative IP adhesion formation by coating the serosal surfaces and decreasing the extent of initial tissue injury.

  6. Comparison of honey and dextrose solution on post-operative peritoneal adhesion in rat model.

    Science.gov (United States)

    Rahimi, Vafa Baradaran; Shirazinia, Reza; Fereydouni, Narges; Zamani, Parvin; Darroudi, Sousan; Sahebkar, Amir Hossein; Askari, Vahid Reza

    2017-08-01

    Peritoneal adhesion between abdominal organs is a complication of surgery. It causes major complications like pain, bowel obstruction, infertility and increases risk of death. Honey is known to have anti-inflammatory and antioxidant properties potentially relevant for adhesive protection. Thirty rats were divided into five groups: negative control without any surgical procedure (normal group), control group treated with normal saline, experimental group I treated with 1ml of 10% honey, experimental group II treated with honey at half concentration of group I (honey0.5), and positive control group receiving 1ml of dextrose 5%. Inflammatory, growth and angiogenesis factors (TNF-α, Il-6, IL-1β, TGF-β1 and VEGF) of the adhesion tissue were assessed using ELISA. Antioxidant factors (NO, GSH and MDA) were also assessed using biochemical procedures. The difference between peritoneal adhesion scores, TNF-α, IL-1β, IL-6, TGF-β1, VEGF, NO, GSH and MDA value of all groups was strongly significant (p<0.001). We showed that honey can decrease peritoneal adhesion (p<0.001), TNF-α (p<0.001), IL-1β (p<0.001), IL-6 (p<0.001), TGF-β1 (p<0.001), VEGF (p<0.001), NO (p<0.001), MDA (p<0.001) and increase GSH (p<0.001) compared with control group. Honey 0.5 also significantly decreased peritoneal adhesion (p<0.001), TNF-α (p<0.001), IL-1β (p<0.01), IL-6 (p<0.001), VEGF (p<0.001), NO (p<0.001), MDA (p<0.01) and increase GSH (p<0.001) compared with control group. We find that honey can decrease inflammatory, growth and angiogenesis factors which can advance peritoneal adhesion and increase antioxidant factors. Honey could serve as a protective agent for peritoneal adhesion. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  7. Effect of hyaluronic acid on postoperative intraperitoneal adhesion formation and reformation in the rat model

    Energy Technology Data Exchange (ETDEWEB)

    Urman, B.; Gomel, V. (Department of Obstetrics and Gynecology, University of British Columbia, Vancouver (Canada))

    1991-09-01

    The local application of 0.25% or 0.4% HA before the induction of a measured laser injury on the rat uterine horn was associated with a significant reduction (P less than 0.05) in postoperative IP adhesions when compared with the group of animals pretreated with the diluent vehicle PBS or received no pretreatment. However, 0.4% HA, when applied in a similar manner, was ineffective in reducing reformation of adhesions after microsurgical adhesiolysis.

  8. Effect of intercellular adhesion molecule 1 expression in radiation otitis media murine model

    International Nuclear Information System (INIS)

    Wang Shengzi; Cheng Qingfang; Lu Shenbin; Liu Jianping; Wang Shuyi

    2003-01-01

    Objective: To characterize the dose- and time-dependent changes in intercellular adhesion molecule 1 (ICAM-1) expression and the role of this molecule as a mediator of middle ear inflammation induced by radiation. Methods: Radiation-induced otitis media animal models were established by using guinea pigs after 60 Co irradiation with 3 Gy/fraction per day, 5 times per week to a total dose of 15, 30, 45 Gy. The expression of ICAM-1 was studied by SP immunohistochemistry with the relation between radiation dose and infiltration of leukocytes investigated. Results: ICAM-1 was not expressed in the normal epithelium of the middle ear mucosa. Mucosal epithelium strongly expressed ICAM-1 after having been administered with 45 Gy of irradiation showing a significant correlation between the expression of ICAM-1 and the infiltration of leukocytes. Conclusions: Irradiation increases the expression of ICAM-1 in t