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Sample records for mimosa scabrella benth

  1. bracatinga (mimosa scabrella bentham

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    Fernando José Fabrowski

    2005-01-01

    Full Text Available The main objective of this research was to compare anatomical wood characteristics of the popular varieties of Mimosa scabrella Bentham known as bracatinga-branca, bracatinga-vermelha and bracatingaargentina. The material used in this work was collected from 6 to 7 years old trees of the popular varieties from areas located in Colombo municipality, State of Paraná, owned by Embrapa Florestas. Botanic for anatomical characterization of wood material was identified and described. Results did not show differences of anatomical characteristics between the bracatinga-branca and bracatinga-vermelha. The bracatingabranca and bracatinga-vermelha showed statistically differences in relation to bracatinga-argentina to average values in pores tangential diameter, pores quantity per square mm, vessel elements individual length, axial parenchyma cells diameter, unisseriate and multisseriate rays width. In conclusion, the observations contribute to a better understanding of the differences showed by the popular varieties.

  2. Modelagem do volume do povoamento para Mimosa scabrella Benth. na regição metropolitana de Curitiba Stand volume modelling of Mimosa scabrella benth. from Curitiba metropolitan region

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    Sebastião do Amaral Machado

    2008-06-01

    Full Text Available O objetivo desta pesquisa foi modelar o volume total com casca do povoamento da bracatinga (Mimosa scabrella Benth.. Coletaram-se dados em bracatingais com regeneração induzida pelo fogo, em 14 municípios da Região Metropolitana de Curitiba (RMC, Estado do Paraná - Brasil. Utilizou-se uma base de dados constituída por 229 unidades amostrais de 100 a 400 m². Em cada parcela, mensurou-se o diâmetro à altura do peito, bem como a altura total de todas as árvores, em bracatingais de 3 a 18 anos de idade. Para a estimativa do volume total com casca, testaram-se vários modelos de regressão selecionados da literatura, o que gerou uma matriz com 58 variáveis independentes, visando construir modelos por meio do método Stepwise. As equações de melhor ajuste foram validadas utilizando-se o teste Qui-quadrado, a análise gráfica de resíduos e o erro-padrão da estimativa. Várias equações tiveram bom desempenho, apresentaram valores de coeficiente de determinação ajustados superiores a 0,995, erro-padrão da estimativa inferior a 3% e adequada distribuição de resíduos. As duas equações de melhor desempenho foram validadas, apresentando erro-padrão da estimativa de 2,37% e 2,13%, valor não-significativo para o teste do Qui-quadrado e equilibrada distribuição dos resíduos.The objective of this research was to model the stand volume outside bark of Mimosa scabrella Benth. stands, from the Curitiba Metropolitan Region (CMR, Parana State - Brazil. To reach this aim, a database of 229 permanent and temporary sample plots measuring from 100 to 400 m² each, from 14 municipalities in the CMR, was used. In each sample plot, the diameter at breast height and the total height of all 3- to 18-year-old bracatinga trees were measured. To estimate the total volume, regression models selected from the literature were tested. A set of 58 independent variables was generated also to construct models by the Stepwise method. The equations of best

  3. Comparison of the susceptibility of two hardwood species, Mimosa scabrella Benth and Eucalyptus viminalis labill, to steam explosion and enzymatic hydrolysis

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    L. P. Ramos

    2000-01-01

    Full Text Available Steam explosion of two hardwood species was carried out with and without addition of sulfuric acid (H2SO4 0.5%, p/v as a pretreatment catalyst. In general, wood chips of Eucalyptus viminalis Labill were shown to be more amenable to pretreatment than chips derived from bolds of Mimosa scabrella Benth (bracatinga. This was apparent from all pretreatment parameters tested including the overall recovery yields of pretreated fractions, carbohydrates (pentoses and hexoses recovered as water-solubles, yield of dehydration by-products and lignin susceptibility to acid hydrolysis. There was no evidence for complete deacetylation of both wood species during pretreatment and lignin appeared to undergo extensive acid hydrolysis at higher pretreatment severities. Steam treatment at 205ºC for 5 min without addition of an acid catalyst was shown to be uncapable of removing the hemicellulose component from bracatinga chips, as determined by chemical analysis of the steam-treated water-insoluble fractions. Nearly 30% of the hemicellulose (xylan found in bracatinga remained unhydrolysed after pretreatment, whereas more than 90% of this component could be removed from eucalypt chips under the same pretreatment conditions. Likewise, pretreatment of eucalypt chips resulted in a more extensive solubilization of glucans (cellulose by acid hydrolysis. Addition of dilute H2SO4 as a pretreatment catalyst generally increased the recovery yield of fermentable sugars in the water-soluble fractions and this effect was more pronounced for the pretreatment of bracatinga chips. Steam-treated substrates produced from bracatinga were also less accessible to enzymatic hydrolysis than those produced from eucalypt chips, regardless of the use of an acid catalyst.Duas espécies de angiospermas foram comparadas em relação à suas susceptibilidades ao pré-tratamento por explosão a vapor. De um modo geral, cavacos industriais de Eucalyptus viminalis Labill apresentaram-se mais

  4. Dinâmica da distribuição diamétrica de bracatingais na região metropolitana de Curitiba Dinamic of the diameter distribuition of Mimosa Scabrella Benth stands in the metropolitam region of Curitiba

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    Sebastião do Amaral Machado

    2006-10-01

    Full Text Available O objetivo desta pesquisa foi estudar a dinâmica da distribuição diamétrica com relação à idade, em povoamentos nativos de bracatinga (Mimosa scabrella Benth. localizados nos municípios da região metropolitana de Curitiba. Os dados utilizados provieram de um experimento de densidades iniciais, delineado em blocos ao acaso, constituído de cinco blocos e quatro tratamentos, perfazendo um total de 20 parcelas de 325 m², as quais foram remedidas às idades aproximadas de 4, 5, 6 e 7,5 anos. A densidade inicial sofreu redução para 2.000, 4.000 e 8.000 árvores por hectare quando o povoamento tinha 1 ano de idade. Deixou-se também uma parcela-testemunha em cada bloco, as quais não sofreram nenhum raleamento e tinham em média 25.000 árvores por hectare. Desses cinco blocos, um estava localizado na classe de sítio I, 2 na classe de sítio II e 2 blocos na classe de sítio III. Paralelamente, foram medidos os diâmetros e as alturas de todas as árvores em 124 parcelas temporárias distribuídas nos vários municípios da região metropolitana de Curitiba, representativos de povoamentos de bracatinga sob regime tradicional de cultivo e manejo, cobrindo idades de 3 a 18 anos, e as três classes de sítio. Dentre as funções de densidade de probabilidade testadas por Bartoszeck (2000, a S B de Johnson foi a de melhor desempenho, sendo, portanto, utilizada para a estimativa do número de árvores por classe diamétrica e por hectare e conseqüente construção das curvas de distribuição diamétrica para as inúmeras combinações de idade, sítio e densidades disponíveis. Através da observação visual das curvas sobrepostas, evidenciou-se que essas apresentaram assimetria para a direita e achatamento (curtose, com o aumento da idade. As curvas foram, então, comparadas analiticamente entre si, pelo teste qui-quadrado, o qual revelou a existência de diferenças significativas entre as curvas de distribuição diamétrica em

  5. ANATOMICAL COMPARISON OF POPULAR BRACATINGA (Mimosa scabrella Bentham) VARIETIES OF WOOD

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    Fernando José Fabrowski; Graciela Inés Bolzon de Muñiz; Maria Cristina M. Mazza; Tomoe Nakashima; Umberto Klock; João Carlos Possamai; Silvana Nisgoski

    2010-01-01

    The main objective of this research was to compare anatomical wood characteristics of the popular varieties of Mimosa scabrella Bentham known as bracatinga-branca, bracatinga-vermelha and bracatinga-argentina. The material  used in this work was collected from 6 to 7 years old trees of the popular varieties from areas located in Colombo municipality, State of Paraná, owned by Embrapa Florestas. Botanic for anatomical characterization of wood material was identified and described. Results did ...

  6. Anatomia da madeira de Mimosa eriocarpa Benth.

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    Celso Carnieletto

    2009-09-01

    Full Text Available Normal 0 21 false false false MicrosoftInternetExplorer4 São descritos os caracteres anatômicos da madeira de Mimosa eriocarpa Benth. e fornecidos 31 dados quantitativos de sua estrutura xilemática. A descrição é ilustrada com fotomicrografias tomadas nos três planos anatômicos. A estrutura da madeira é comparada com referências da literatura para espécies afins, em uma análise de caráter taxonômico-filogenética.

  7. ANATOMICAL COMPARISON OF POPULAR BRACATINGA (Mimosa scabrella Bentham VARIETIES OF WOOD

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    Fernando José Fabrowski

    2010-08-01

    Full Text Available The main objective of this research was to compare anatomical wood characteristics of the popular varieties of Mimosa scabrella Bentham known as bracatinga-branca, bracatinga-vermelha and bracatinga-argentina. The material  used in this work was collected from 6 to 7 years old trees of the popular varieties from areas located in Colombo municipality, State of Paraná, owned by Embrapa Florestas. Botanic for anatomical characterization of wood material was identified and described. Results did not show differences of anatomical characteristics between the bracatinga-branca and bracatinga-vermelha. The bracatinga-branca and bracatinga-vermelha showed statistically differences in relation to bracatinga-argentina to average values in pores tangential diameter, pores quantity per square mm, vessel elements individual length, axial parenchyma cells diameter, unisseriate and multisseriate rays width. In conclusion, the observations contribute to a better understanding of the differences showed by the popular varieties.

  8. Overcome dormancy and in vitro germination of seeds of Mimosa scabrella BenthamSuperação da dormência e germinação in vitro de sementes de bracatinga (Mimosa scabrella Bentham

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    Marlove Fátima Brião Muniz

    2012-06-01

    Full Text Available Mimosa scabrella Bentham is a tree native to Brazil of ecological and economic importance, whose production of quality seedlings can be performed via tissue culture. This work aimed to select a method to overcome dormancy and to establish the concentration of salts in the MS nutritive medium best suited for in vitro germination of seeds of M. scabrella. Were evaluated four treatments to overcome dormancy: mechanical scarification; immersion in water at room temperature for 24 hours; immersion in hot water (80°C for 5 minutes, and control, arranged in a randomized design with four replicates of 50 seeds. After seven days, we evaluated the germination and at 14 days, abnormal seedlings, dead and firm seeds. In one the second assay, after disinfestation and mechanical scarification, seeds were cultivated in MS nutritive medium in four concentrations of salts, according to treatment: 1/8, 1/4, 1/2 or full (100%, arranged in completely randomized design with 25 replicates, each with three seeds. At 25 days of culture, we evaluated the in vitro germination of seeds. Among the tested methods to overcome dormancy, mechanical scarification was the most efficient. The maximum in vitro germination was observed in the medium 1/4 MS. The maximum in vitro germination was observed in the medium 1/4 MS. The mechanical scarification with sandpaper provides the highest germination rate, while the maximum in vitro germination is observed using ¼ MS medium. A bracatinga, Mimosa scabrella Bentham, é uma árvore nativa do Brasil de importância ecológica e econômica, cuja produção de mudas de qualidade pode ser efetuada via cultura de tecidos. Este trabalho objetivou selecionar um método para a superação de dormência, bem como estabelecer a concentração de sais do meio nutritivo MS mais adequada para a germinação in vitro das sementes de bracatinga. Foram avaliados quatro tratamentos para superação: escarificação mecânica; imersão em água

  9. EFFECTIVENESS OF A OIL RESIDUE FROM PETROLEUM ON DIMENSIONAL STABILITY OF Pinus sp. (Pinus AND Mimosa scabrella Bentham (Bracatinga WOOD

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    Alexandre Florian da Costa

    2010-08-01

    Full Text Available In search for alternative uses of a petroliferous oily residue known as “LCO” (Liquid Cycle Oil, its capacity to improve the dimensional stability of the wood was evaluated using Pinus sp. (pinus and Mimosa scabrella Bentham (bracatinga. The LCO was tested in its original composition and also diluted in different proportions into a commercial kerosene. The dimensional stability in the three anatomic axis, volumetric changes, shrinkage and anisotropy coefficients, basic density and 12% relative humidity density changes were evaluated, in different stages during the investigation process. The results show no significant differences in maximum swelling and shrinkage between treated and untreated wood blocks at 5% probability level for both species. However, a reduction in the shrinkage coefficient was observed with the increase of LCO concentration, for both species. In general, pinus showed lowest values in all parameter evaluated. The shrinkage and anisotropy coefficient were higher for bracatinga than pinus, nevertheless, no significant differences were observed between treated and untreated wood blocks. These results indicated that changes between tangential and radial faces were minimal, possible due to a poor penetration of LCO into the cell walls. This result, as a consequence, could be associated with a weak performance of the tested chemical, which could not improve the dimensional stability of wood for both species. The increasing LCO concentrations increased the density of both species at 12% relative humidity. This result could be associated to the presence of part of the chemical remaining in the walls of the cellular lumen and the resiniferous channels. Besides, the external aspect of the treated wood was also other restrictive factor to the use of LCO.

  10. Estudo anatômico do xilema secundário de Mimosa trachycarpa Benth Anatomy of the secondary xylem of Mimosa trachycarpa Benth

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    José Newton Cardoso Marchiori

    1997-06-01

    Full Text Available São descritos os caracteres microscópicos da madeira de Mimosa trachycarpa Benth., fornecidos dados quantitativos, determinações estereológicas e fotomicrografias de sua estrutura anatômica. Foram encontrados raios muito finos, parênquima paratraqueal, fibras libriformes não septadas, pontoações ornamentadas e poros agrupados em cachos, com arranjo tendente a diagonal. A estrutura da madeira é comparada com referências da literatura para a família Leguminosae e gênero Mimosa, em análise filogenética, taxonômica e ecológica.The wood anatomy of Mimosa trachycarpa Benth. is described. Photomicrographs as well as quantitative and stereological data of its minute structure were furnished. Very fine rays, paratracheal parenchyma, libriform and not septated fibres, vestured pits and pores in clusters, tending to a diagonal pattern, were found. The wood anatomy was checked with literature of the Leguminosae family and genus Mimosa, considering its phylogenetical, taxonomical and ecological aspects.

  11. Anatomia do xilema secundário de Mimosa incana (Spreng. Benth.

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    José Newton Cardoso Marchiori

    2009-09-01

    Full Text Available Normal 0 21 false false false MicrosoftInternetExplorer4 São descritos os caracteres anatômicos do xilema secundário de Mimosa incana (Spreng. Benth. e fornecidas 29 medições de sua estrutura. A descrição é ilustrada com fotomicrografias. A estrutura anatômica indica um alto grau de especialização filogenética e reúne diversos caracteres indicativos da família Leguminosae, sub-família Mimosoideae e série Lepidotae Benth. Normal 0 21 false false false MicrosoftInternetExplorer4

  12. Índice de diversidade para entomofauna da bracatinga (Mimosa scabrella Benth..

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    Ervandil Corrêa Costa

    2009-09-01

    Full Text Available Normal 0 21 false false false MicrosoftInternetExplorer4 No período de abril de 1983 a setembro de 1984, efetuou-se o levantamento da entomofauna da bracatinga, em Almirante Tamandaré, PR. Os levantamentos foram efetuados quinzenalmente em copas de talhões com 8, 42 e 60 meses de idade, através da rede de copa e, de seus respectivos sub-bosques com rede de varredura. Para o cálculo do índice de diversidade utilizou-se a fórmula  alfa = (S-1/log N. Para a tabulação dos dados, optou-se pelas ordens Homoptera, Hemíptera e Coleóptera que apresentaram maior consistência quali-quantitativa. De acordo com os resultados obtidos ficou evidenciado que com o aumento da idade da planta e de seu respectivo sub-bosque ocorre um aumento concomitante e gradativo da diversidade das espécies. Com relação aos dois ecossistemas estudados o sub-bosque apresentou um valor  maior  para  o  índice de diversidade e no que se refere a diversidade das espécies entre famílias, separadamente, Chrysomelidae destacou-se com o mais alto  índice para o ecossistema formado pelas copas e, entre os diferentes sub-bosques destacou-se a família Curculionidae.

  13. Efectos centrales de los constituyentes de Mimosa opthalmocentra Mart. ex Benth

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    Batista,Leônia Maria; Nóbrega de Almeida, Reinaldo

    1997-01-01

    La fracción conteniendo los alcaloides totales (FTA) obtenidos de Mimosa opthalmocentra Mart. ex Benth. en dosis de 50 y 100 mg/kg por vía intraperitoneal (v. ip.) y N, N- Dimetiltriptamina (DMT) en dosis de 32,64 y 128 mg/kg (v. ip.) produjo, en ratones, el "síndrome serotonérgico". En el caso de hordenina, el otro compuesto obtenido de M. Opthalmocentra, no mostró diferencias significativas en relación a los animales del grupo utilizado como control. En los ratones tratados con quetanserina...

  14. Topical anti-inflammatory activity of a monofloral honey of Mimosa scabrella provided by Melipona marginata during winter in southern Brazil.

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    Borsato, Débora M; Prudente, Arthur S; Döll-Boscardin, Patrícia M; Borsato, Aurélio V; Luz, Cynthia F P; Maia, Beatriz H L N S; Cabrini, Daniela A; Otuki, Michel F; Miguel, Marilis D; Farago, Paulo V; Miguel, Obdulio G

    2014-07-01

    Melipona marginata is an endangered species of stingless bee from Brazil that produces honey with particular physicochemical features and a remarkable exotic flavor. To the best of our knowledge, this is the first report devoted to exploring the medicinal potential of this honey. Thus, the aim of this paper was to investigate the potential anti-inflammatory activity of honey extract from M. marginata on skin inflammation. The honey sample was classified as a monofloral honey of Mimosa scabrella. The presence of 11 phenolic compounds as kaempferol and caffeic acid was detected using the high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-UV-ESI-MS) method. The anti-inflammatory activity was measured using a 12-O-tetradecanoylphorbol-13-acetate-induced ear edema model of inflammation in mice. The topical application of the M. marginata honey extract (1.0 mg/ear) was able to reduce ear edema with an inhibitory effect of 54 ± 5%. This extract decreased the myeloperoxidase activity in 75 ± 3%, which suggests a lower leucocyte infiltration that was confirmed by histological analysis. This extract also provided a reduction of 55 ± 14% in the production of reactive oxygen species. This anti-inflammatory activity could be due to a synergic effect of the phenolic compounds identified in the honey sample. Taken together, these results open up new possibilities for the use of M. marginata honey extract in skin disorders.

  15. Estudo comparativo da madeira de Mimosa ophthalmocentra Mart. ex Benth e Mimosa tenuiflora (Willd.) Poir. (Fabaceae-Mimosoideae) na caatinga nordestina Comparative study of Mimosa ophthalmocentra Mart. ex Benth and Mimosa tenuiflora (Willd.) Poir. (Fabaceae-Mimosoideae) wood in the caatinga of Northeast Brazil

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    Lazaro Benedito da Silva; Francisco de Assis Ribeiro dos Santos; Peter Gasson; David Cutler

    2011-01-01

    Pela importância econômica da madeira de algumas espécies do gênero Mimosa L. ocorrentes na caatinga nordestina e pelo fato de Mimosa ophthalmocentra Mart. ex Benth. (jurema-de-imbira), às vezes, ser confundida com Mimosa tenuiflora (Willd.) Poir. (jurema-preta) ou vice-versa, este trabalho objetivou apresentar novos dados referentes à anatomia e densidade básica da madeira das duas espécies, visando oferecer subsídio para identificação das duas espécies, identificar caracteres anatômicos da ...

  16. A estrutura do sub-bosque de povoamentos homogêneos de Mimosa scabrella bentham, em área minerada, em Poços de Caldas, MG

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    Mauro Eloi Nappo

    2000-01-01

    Full Text Available A floristic and structural survey of the natural regeneration of shrubby and arboreal species of the understory of an established homogeneous plantation of Mimosa scabrella Bentham was corried out viewing, the reclamation of a mined area, in Poços de Caldas, Minas Gerais State, Brazil. Nineteen plots of 50 m2 (5 m × 10 m were used and the shrubby and arboreal individuals measuring equal or above 30 cm were sampled and 1,946 individuals belonging to 63 botanic families were found. Soil samples were collected at a depth of 0 to 20 cm, in each of the 19 plots, and the contents of sand, silt,clay, organic matters, pH nitrogen, phosphorus, potassium, calcium and magnesium were analysed. The influence of the soil variables upon the density of the sampled species was analysed using the Canonical Correspondence Analysis (CCA. A significative correlation was verified among these variables at a level of 5% of probability using the Monte Carlo test. The species Miconia sellowiana, Miconia pepericarpa, Cestrum amictum, Alchornea triplinervia, Cordia superba and Casearia sylvestris presented a close to indifferent behaviour in relation to the soil variables studied, and these species largely surpassed the other species in relation to the floristic and the strutured parameters. This behaviour reinforces the indication of the species, made by NAPPO (1999, as having a potential for use in mixed and enrichment plantation in condition similar to those of the studied area. The identification and mensuration of the enviromental variables and of the history of the area are important pieces for the understanding of the process of population dynamics, and particularly, for degraded area getting to the reclamation phase.

  17. THE STRUCTURE OF THE UNDERSTOREY OF PURE STAND OF Mimosa scabrella Bentham IN MINED AREA, IN POÇOS DE CALDAS, BRAZIL

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    Mauro Eloi Nappo

    2009-10-01

    Full Text Available A floristic and structural survey of the natural regeneration of shrubby and arboreal species of the understory of an established homogeneous plantation of Mimosa scabrella Bentham was corried out  viewing, the  reclamation  of  a  mined  area,  in  Poços de  Caldas, Minas  Gerais State, Brazil. Nineteen  plots of 50 m2 (5 m × 10 m were used and the shrubby and arboreal individuals measuring equal or above 30 cm were sampled and 1,946 individuals belonging to 63 botanic families were found. Soil samples were collected at a depth of 0 to 20 cm, in each of the 19 plots, and the contents of sand, silt,clay, organic matters, pH nitrogen, phosphorus, potassium, calcium and magnesium were analysed. The influence of the soil variables upon the density of the sampled species was analysed using the Canonical Correspondence Analysis (CCA. A significative correlation was verified among these variables at a level of 5% of probability using the Monte Carlo test. The species Miconia sellowiana, Miconia pepericarpa, Cestrum amictum, Alchornea triplinervia, Cordia superba and Casearia sylvestris presented a close to indifferent behaviour in relation to the soil variables studied, and these species largely surpassed the other species in relation to the floristic and the strutured parameters. This behaviour reinforces the indication of the species, made by NAPPO (1999, as having a potential for use in mixed and enrichment plantation in condition similar to those of the studied area. The identification and mensuration of the enviromental variables and of the history of the area are important pieces for the understanding of the process of population dynamics, and particularly, for degraded area getting to the reclamation phase.

  18. Chemical constituents and toxicological studies of leaves from Mimosa caesalpiniifolia Benth., a Brazilian honey plant

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    Monção, Nayana Bruna Nery; Costa, Luciana Muratori; Arcanjo, Daniel Dias Rufino; Araújo, Bruno Quirino; Lustosa, Maria do Carmo Gomes; Rodrigues, Klinger Antônio da França; Carvalho, Fernando Aécio de Amorim; Costa, Amilton Paulo Raposo; Lopes Citó, Antônia Maria das Graças

    2014-01-01

    Background: Mimosa caesalpiniifolia Benth. (Leguminosae) is widely found in the Brazilian Northeast region and markedly contributes to production of pollen and honey, being considered an important honey plant in this region. Objective: To investigate the chemical composition of the ethanol extract of leaves from M. caesalpiniifolia by GC-MS after derivatization (silylation), as well as to evaluate the in vitro and in vivo toxicological effects and androgenic activity in rats. Materials and Methods: The ethanol extract of leaves from Mimosa caesalpiniifolia was submitted to derivatization by silylation and analyzed by gas chromatography-mass spectrometry (GC-MS) to identification of chemical constituents. In vitro toxicological evaluation was performed by MTT assay in murine macrophages and by Artemia salina lethality assay, and the in vivo acute oral toxicity and androgenic evaluation in rats. Results: Totally, 32 components were detected: Phytol-TMS (11.66%), lactic acid-2TMS (9.16%), α-tocopherol-TMS (7.34%) and β-sitosterol-TMS (6.80%) were the major constituents. At the concentrations analyzed, the ethanol extract showed low cytotoxicity against brine shrimp (Artemia salina) and murine macrophages. In addition, the extract did not exhibit any toxicological effect or androgenic activity in rats. Conclusions: The derivatization by silylation allowed a rapid identification of chemical compounds from the M. caesalpiniifolia leaves extract. Besides, this species presents a good safety profile as observed in toxicological studies, and possess a great potential in the production of herbal medicines or as for food consumption. PMID:25298660

  19. A ESTRUTURA DO SUB-BOSQUE DE POVOAMENTOS HOMOGÊNEOS DE Mimosa scabrella Bentham, EM ÁREA MINERADA, EM POÇOS DE CALDAS, MG

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    Mauro Eloi Nappo

    2000-07-01

    Full Text Available Foi realizado um inventário florístico-estrutural da regeneração natural de espécies arbustivo-arbóreas do sub-bosque de um plantio homogêneo de Mimosa scabrella Bentham implantado, visando à reabilitação de área minerada, em Poços de Caldas. Foram utilizadas dezenove parcelas de 50 m2 (5 m × 10 m e amostrados os indivíduos arbustivo-arbóreos com altura igual ou superior a 30cm, tendo sido encontrados 1.946 indivíduos, pertencentes a 63 famílias botânicas. Amostras de solo foram coletadas à profundidade de 0 cm a 20 cm, em cada uma das dezenove parcelas e analisados os teores de areia, silte, argila, matéria orgânica, pH, nitrogênio, fósforo, potássio, cálcio e magnésio. Foi analisada a influência de variáveis edáficas sobre a densidade das espécies amostradas, utilizando Análise de Correspondência Canônica ("Canonical Correspondence Analysis" - CCA. Foi verificada correlação significativa entre elas a 5% de probabilidade pelo teste de Monte-Carlo. As espécies Miconia sellowiana, Miconia pepericarpa, Cestrum amictum, Alchornea triplinervia, Cordia superba e Casearia sylvestris apresentaram comportamento próximo ao indiferente em relação às variáveis edáficas estudadas, sendo que estas se destacam de forma superior em relação às demais espécies quanto aos parâmetros florístico-estruturais. Esse comportamento reforça a indicação de tais espécies, feita por NAPPO (1999, como de potencial para uso em plantios mistos e de enriquecimento em condições similares às da área estudada. A identificação e mensuração de outras variáveis ambientais e do histórico da área são peças importantes para o entendimento dos processos de dinâmica de povoamentos e, em particular, para áreas degradadas em fase de reabilitação.

  20. Dinâmica da estrutura fitossociológica da regeneração natural em sub-bosque de Mimosa scabrella Bentham em área minerada, em Poços de Caldas, MG Phytosociology structure dynamics of natural regeneration in understory of Mimosa scabrella Bentham in mined area, Poços de Caldas, Brazil

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    Mauro Eloi Nappo

    2004-12-01

    Full Text Available Como estratégia de reabilitação de área minerada foram realizados, no ano agrícola 1982/1983, hidrossemeadura de gramíneas e leguminosa e o plantio puro de Mimosa scabrella Bentham em Poços de Caldas, MG. Em 1997 foi implantado um conjunto de 19 parcelas permanentes de 50 m² nessa área, para caracterização inicial do processo de regeneração natural. Em 2000, foi realizado o segundo inventário nas parcelas, para caracterização do processo de dinâmica da regeneração natural, que é o objeto deste trabalho. O processo de dinâmica da regeneração natural foi caracterizado mediante análises quantitativas e qualitativas da composição florística e da estrutura horizontal e vertical. O povoamento florestal do Retiro-Branco está sobre intensa atividade de estruturação, caracterizando o estágio inicial do processo de sucessão. O declínio do povoamento puro de Mimosa escabrella está modificando a ordem anteriormente estabelecida para o processo de sucessão da área, provocando a diversificação de condições de sítio e, assim, selecionando a ocupação deste em função dos grupos ecológicos, sendo as espécies pioneiras as mais favorecidas. As espécies secundárias são as de maior dominância nas maiores classes de altura e de diâmetro, sendo as principais responsáveis pela edificação do estrato superior, em especial a espécie Miconia sellowiana. As espécies que apresentaram melhor desempenho na colonização e estruturação da regeneração natural do Retiro-Branco, nos dois inventários, foram Miconia sellowiana, Psychotria sessilis, Leandra melastomoides, Clethra scabra, Myrsine umbellata, Miconia pepericarpa, Tibouchina candolleana, Cordia superba, Cestrum amictum, Alchornea triplinervia, Casearia sylvestris, Blepharocalyx salicifolius, Myrcia rostrata e Schinus terebinthifolius, sendo indicadas como espécies para uso nos programas de reabilitação de áreas mineradas em condições semelhantes sobre a

  1. BEHAVIOR OF BRACATINGA (Mimosa scabrella AND SESBANIA (Sesbania sesban IN DEGRADED AREAS BY DISPOSAL RESIDUE FROM BAUXITE PROCESSING = COMPORTAMENTO DE BRACATINGA (Mimosa scabrella E SESBÂNIA (Sesbania sesban EM ÁREAS DEGRADADAS PELA DISPOSIÇÃO DE RESÍDUOS PROVENIENTES DO PROCESSAMENTO DA BAUXITA

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    Maria Madalena Ferreira Chaves

    2002-01-01

    Full Text Available Topsoil return in mining activities is a common and extensively usedprocedure. But, for some companies this operation may represent around 70-80% of all costs to reclaim degraded areas. This study evaluated the contribution of two leguminous species to improve the initial colonization of a damaged land by bauxite residue disposalafter processing in Poços de Caldas-MG. Plants of Mimosa scabrella ("bracatinga" e Sesbania sesban ("sesban" were planted in January of 1999. Plants were spaced in 2 x 1,5 m and each plot received 25 plants. The statistical alignment was of randomized blocks in the factorial proportion (3 x 4 x 2, with three of the gypsum dosage, four for the thickness layer of superficial soil laid over the residue and two species. Four repetitions were done, with five plants each. Measurements of total height were made and mortality rates were calculated. The results showed that the residue presented similarproperties to a sodium-salt soil, with high pH value and electric conductivity. It was also observed that the gypsum doses, the layer thickness, and the species has significant influences in the average values registered for the variables studied. An inversely proportional relation was observed between the mortality rate and the variable gypsum dosage and layer thickness of the utilized soil, in which the increase of gypsum dosage and layer width corresponded to decreased mortality rate for the species. For all the variables studied, "sesban" showed better performance than "bracatinga". = O retorno de horizontes superficiais do solo para áreas degradadas poratividade de disposição de resíduos tem sido prática rotineira e largamente utilizada. Para algumas empresas, no entanto, essa operação tem representado cerca de 70 a 80% dos custos finais de recuperação de áreas mineradas. O presente trabalho teve como objetivoavaliar a contribuição de duas espécies leguminosas na colonização inicial de um solo degradado pela

  2. RESISTÊNCIA NATURAL DA MADEIRA DE SABIÁ (Mimosa caesalpiniifolia Benth. A CUPINS SUBTERRÂNEOS

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    FRANCISCO HUGO HERMÓGENES DE ALENCAR

    2011-01-01

    Full Text Available The objective of this research was to evaluate the wood natural resistance of Mimosa caesalpiniifolia Benth. of phenotypes (plants with and without prickles to subterranean termite (Nasutitermes corniger Motsch. in forced feeding and feeding preference assays, under laboratory conditions. Wood test samples measuring 2.54 x 1.50 x 0.64 cm (forced feeding and 10.00 x 1.50 x 0.64 cm (feeding preference, with the largest measurement in the fiber direction, were obtained from three positions from pith to bark direction. The samples were exposed for 28 days (forced feeding and 45 days (feeding preference to Nasutitermes corniger Motsch. termites. In forced feeding assay the termites caused superficial attack in wood and lived during 8 to 10 days, thus the wood was classified as resistant. In forced feeding assay more mass loss and attack in wood of external positions to both phenotypes was observed. To the waste and survival time of termites were similar to both types. In feeding preference a larger mass loss and waste to inner position in both phenotypes was observed. In general, the plants with prickles lost more mass than the one without prickles. Therefore, the wood of plants without prickles is more suitable to be use in construction of fences, sheepfolds and other similar uses where the wood shall be subject to attack by termites.

  3. GERMINAÇÃO DE SEMENTES DE SABIÁ (MIMOSA CAESALPINIAEFOLIA BENTH. E ALGAROBA (PROSOPIS JULIFLORA (SW DC

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    Salvador Barros Torres

    1994-12-01

    Full Text Available Estudou-se o efeito da temperatura e do substrato sobre a germinação de sementes de sabiá (Mimosa caesalpiniaefolia Benth. e algaroba (Prosopis juliflora (SW DC em condições de laboratório. Foram testadas as temperaturas de 25°C e 30°C constantes e 20-30°C alternadas em substratos de papel toalha, papel mata-borrão e areia. O melhor resultado de germinação para as sementes de sabiá foi obtido com a temperatura de 20-30°C em substrato de papel mata-borrão e a mesma temperatura em substrato de areia, para sementes de algaroba.

  4. A jurema preta (Mimosa hostilis, Benth) como fonte energetica do semi-arido do nordeste - carvão

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    Faria, Washington Luiz Fonseca

    2013-01-01

    A situação sócio-econômica da região semi-árida do nordeste e a restrita utilização do potencial da vegetação da caátinga, devido ao pequeno porte, associado as necessidades de se buscar fontes alternativas de energia, despertou a necessidade de conhecimentos sobre a espécie Jurema Preta (Mimosa hostilis, .Benth) na produção de carvão vegetal. A carbonização foi conduzida em retorta elétrica, escala de laboratório, variando-se as condições de carbonização. Foi utilizado as temperaturas de 400...

  5. Estudo comparativo da madeira de Mimosa ophthalmocentra Mart. ex Benth e Mimosa tenuiflora (Willd. Poir. (Fabaceae-Mimosoideae na caatinga nordestina Comparative study of Mimosa ophthalmocentra Mart. ex Benth and Mimosa tenuiflora (Willd. Poir. (Fabaceae-Mimosoideae wood in the caatinga of Northeast Brazil

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    Lazaro Benedito da Silva

    2011-06-01

    Full Text Available Pela importância econômica da madeira de algumas espécies do gênero Mimosa L. ocorrentes na caatinga nordestina e pelo fato de Mimosa ophthalmocentra Mart. ex Benth. (jurema-de-imbira, às vezes, ser confundida com Mimosa tenuiflora (Willd. Poir. (jurema-preta ou vice-versa, este trabalho objetivou apresentar novos dados referentes à anatomia e densidade básica da madeira das duas espécies, visando oferecer subsídio para identificação das duas espécies, identificar caracteres anatômicos da madeira relacionando-os com o ambiente caatinga e apresentar o potencial energético que sua madeira possui. O estudo anatômico e a determinação da densidade da madeira foram realizados com amostras ao nível do peito (1,30 m acima do solo e em dois galhos com diferentes diâmetros, de espécimes ocorrentes nos municípios de Serra Talhada e Sertânia, Pernambuco, Brasil. As espécies apresentam distinção expressa através do tipo de casca, coloração do cerne e do alburno, além das características peculiares da madeira. Mimosa ophthalmocentra apresenta camadas de crescimento distintas, constituídas por linhas de parênquima axial contendo cristais, parênquima axial escasso e menor quantidade de raios por mm². Já Mimosa tenuiflora apresenta camadas de crescimento distintas, porém sem cristais, parênquima axial vasicêntrico, em faixas ou aliforme confluente, e maior percentagem de raios. Ambas apresentam algumas estruturas da madeira com as características anatômicas comuns às diversas espécies do gênero Mimosa, contribuindo assim para uma melhor caracterização do gênero. Pelos parâmetros anatômicos da madeira e pela elevada densidade básica (>0,84 g/cm³, concluiu-se que as duas espécies apresentam perspectivas seguras para a produção de álcool combustível e carvão vegetal desde a fase de lenho juvenil. Mimosa ophthalmocentra apresenta maior potencial energético, pela maior percentagem de fibras e por possuir par

  6. Arsenic toxicity in Acacia mangium willd. and mimosa Caesalpiniaefolia benth. seedlings

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    Henrique Nery Cipriani

    2013-10-01

    Full Text Available Acacia mangium and Mimosa caesalpiniaefolia are fast-growing woody fabaceous species that might be suitable for phytoremediation of arsenic (As-contaminated sites. To date, few studies on their tolerance to As toxicity have been published. Therefore, this study assessed As toxicity symptoms in A. mangium and M. caesalpiniaefolia seedlings under As stress in a greenhouse. Seedlings of Acacia mangium and M. caesalpiniaefolia were grown for 120 d in an Oxisol-sand mixture with 0, 50, 100, 200, and 400 mg kg-1 As, in four replications in four randomized blocks. The plants were assessed for visible toxicity symptoms, dry matter production, shoot/root ratio, root anatomy and As uptake. Analyses of variance and regression showed that the growth of A. mangium and M. caesalpiniaefolia was severely hindered by As, with a reduction in dry matter production of more than 80 % at the highest As rate. The root/shoot ratio increased with increasing As rates. At a rate of 400 mg kg-1 As, whitish chlorosis appeared on Mimosa caesalpiniaefolia seedlings. The root anatomy of both species was altered, resulting in cell collapse, death of root buds and accumulation of phenolic compounds. Arsenic concentration was several times greater in roots than in shoots, with more than 150 and 350 mg kg-1 in M. caesalpiniaefolia and A. mangium roots, respectively. These species could be suitable for phytostabilization of As-contaminated sites, but growth-stimulating measures should be used.

  7. Emergence and initial development of Mimosa caesalpiniifolia Benth. seedlings for different substrates = Emergência e desenvolvimento inicial de plântulas de Mimosa caesalpiniifolia Benth. em função de diferentes substratos

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    Rômulo Magno Oliveira de Freitas

    2012-04-01

    Full Text Available The present study assessed the effects of different substrates on the emergence and initial development of Mimosa caesalpiniifolia Benth seedlings. For this purpose an experiment was done in a greenhouse of the Department of Plant Sciences of Universidade Federal Rural do Semi-Árido (UFERSA, Mossoró-RN. The statistical design was completely randomized (CRD, where treatments were composed of nine substrates (T1: vermiculite, T2: coconut fiber, T3: trade hortimix®, T4: vermiculite, coconut fiber and compost (1: 1:1, T5: coconut fiber and compost (1:1, T6: coconut fiber and compost (1:2,T7: vermiculite and compost (1:1, T8, vermiculite and compost organic (1:2 and T9: organic compost. All treatments were represented by four replications containing 25 seeds. The following variables were evaluated: emergency percentage, emergence rate index, length of shoot and root, stem diameter, dry leaves, stem, root and total leaf area, specific leaf area, leaf area ratio, and leaf weight ratio. Data were submitted to the Tukey test at 5% probability. The coconut fiber substrates, commercial hortimix® and vermiculite proved superior promoted good emergence and early development of seedlings. The mixture of vermiculite, coconut fiber and compost (1:1:1, coconut fiber and compost (1:1, coconut fiber and compost (1:2 and organic compost and vermiculite (1: 1 was not shown to be adequate for the cultivation of seedlings. There was no emergency in the pure organic compound in the mixture of compost and vermiculite (1:2.Key words - Vermiculite. Organic compost. Coconut fiber. Sabiá. Substrates. = Objetivou-se com o presente trabalho avaliar o efeito de diferentes substratos na emergência e desenvolvimento inicial de plântulas de sabiá (Mimosa caesalpiniifolia Benth.. Para isso, foi instalado um experimento em casa de vegetação do Departamento de Ciências Vegetais da Universidade Federal Rural do Semi-árido (UFERSA, Mossoró-RN. O delineamento estat

  8. Abordagem fitoquímica, composição bromatológica e atividade antibacteriana de Mimosa tenuiflora (Wild Poiret E Piptadenia stipulacea (Benth Ducke =Phytochemical approach, bromatologic composition and antibacterial activity of Mimosa tenuiflora (Wild Poiret and Piptadenia stipulacea (Benth Ducke

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    Denise Aline Casimiro Bezerra

    2011-01-01

    Full Text Available A necessidade de encontrar novas drogas eficazes no combate microbiano tem aumentado a cada dia e estimulado a busca de novos compostos naturais com atividades biológicas. Neste trabalho, realizaram-se estudo fitoquímico e análises microbiológicas com os extratos etanólicos das espécies (jurema-preta Mimosa tenuiflora (Wild Poiret e (juremabranca Piptadenia stipulacea (Benth Ducke, frente a linhagens de bactérias patogênicas. O pó da casca do caule de ambas as espécies foi submetido à avaliação bromatológica e determinados os teores de Matéria Seca, Matéria Mineral, Proteína Bruta, Fibra em Detergente Neutro e Energia Bruta. Os resultados para a prospecção química indicaram a presença de taninos e outros compostos fenólicos, bem como a presença de saponinas em ambos os extratos. Os extratos das duas espécies demonstraram que mais de uma parte das plantas possui atividade antimicrobiana. A composição bromatológica da casca do caule de jurema-preta e jurema-branca apresentou teores diferenciados para as variáveis avaliadas.The need to find new efficient drugs to combat microbes has increasedthe search for new natural compounds with biological activities. In this work, phytochemical studies and microbiological analysis were carried out with the ethanol extracts of Mimosa tenuiflora (Wild Poiret and Piptadenia stipulacea (Benth Ducke on pathogenic bacteria strains. The bark powders of both species were submitted to bromatologic evaluation and the levels of dry matter, mineral matter, crude protein, neutral detergent fiber (NDF and crude energy were determined. The results of the chemical search chemical showed the presence of tannins and other phenolic compounds as well as the presence of saponins in both extracts. The microbiologic evaluation of the extracts of both species showed that more than one part of the plants had antimicrobial activity. The bromatologic composition of the bark powder of Mimosa tenuiflora (Wild

  9. Avaliação da atividade antioxidante e antimicrobiana dos extratos e frações orgânicas de Mimosa caesalpiniifolia Benth. (Mimosaceae

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    Marcelo José Dias Silva

    2012-04-01

    Full Text Available A espécie Mimosa caesalpiniifolia Benth. (Mimosaceae, conhecida popularmente como sabiá e cerva viva, é uma planta arbórea encontrada na caatinga nordestina brasileira, amplamente utilizada pela população na forma de infusões para o tratamento de feridas, bronquites e anti-inflamatório. Diante do exposto, os objetivos deste estudo, foram determinar as atividades antioxidantes e antimicrobianas do extrato etanólico das folhas (EHM, caules (EHL, cascas do caule (EHC, raízes (EHR e frações obtidas das folhas de M. caesalpiniifolia Benth. A atividade antioxidante foi avaliada através do método de captação do radical DPPH, enquanto a atividade antimicrobiana foi avaliada pelo método de microdiluição em caldo, sobre leveduras, bactérias Gram-positivas e Gram-negativas. A capacidade antioxidante mostrou que a fração acetato de etila (Fr-EtOAc foi diretamente proporcional ao teor de polifenóis totais com IC50 de 20,08 ± 0,10 µg/ mL e 721,29±0,60 mg de EAG (equivalentes de ácido gálico por g de extrato. Na atividade antimicrobiana, todos os extratos e frações exibiram atividade inibitória de crescimento frente aos micro-organismos microrganismos avaliados e em concentrações variando de 5 a 1000 μg/mL. A Fr-EtOAc apresentou valores promissores de inibição de crescimento frente a fungos, como Candida glabrata (ATCC 90030 e Candida krusei (ATCC 6258, com concentrações de 20 e 40 μg/mL, respectivamente. Estes resultados são importantes, pois são os primeiros a serem realizados com a espécie M. caesalpiniifolia.

  10. Dinâmica da estrutura diamétrica da regeneração natural de espécies arbóreas e arbustivas no sub-bosque de povoamento puro de Mimosa scabrella Bentham, em área minerada, em Poços de Caldas, MG Diametric struture dynamics for tree and shrub natural regeneration understory within pure stands of Mimosa scabrella Bentham planted on a mined-out site at Poços de Caldas, Minas Gerais state

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    Mauro Eloi Nappo

    2005-02-01

    Full Text Available A área do Retiro-Branco, de propriedade da Companhia Geral de Minas, subsidiária da ALCOA Alumínio S.A., foi minerada para a extração de bauxita de 1978 até 1981. Em 1982/1983, essa área teve seu processo de reabilitação implementado mediante a hidrossemeadura de gramíneas e leguminosas e o plantio puro de Mimosa scabrella Bentham. Em 1997 foi realizado o primeiro inventário da regeneração natural do Retiro-Branco, aravés da implantação de 19 parcelas permanentes para caracterização inicial do processo de sucessão natural. E em 2000 foi realizado o segundo inventário dessas parcelas para caracterizar o processo de dinâmica da regeneração, o objeto deste trabalho. O processo de dinâmica da regeneração natural foi caracterizado mediante análises quantitativas e qualitativas da distribuição diamétrica. Os estudos indicaram que o monitoramento mediante inventário florestal contínuo, com parcelas permanentes setorizadas, é uma ferramenta viável para avaliações da dinâmica da regeneração natural, rumo à compreensão dos caminhos da sucessão. Foi caracterizado o processo de estratificação da regeneração natural, em que as espécies pioneiras e clímax foram as principais componentes do estrato inferior e as secundárias, as principais componentes do estrato superior. O povoamento florestal do Retiro-Branco está sobre intensa atividade de estruturação, caracterizando o estágio inicial do processo de sucessão. As espécies secundárias são as de maior dominância nas maiores classes de altura e de diâmetro, sendo as principais responsáveis pela edificação do estrato superior. A prognose da distribuição diamétrica, realizada mediante o emprego da Cadeia de Markov, foi uma ferramenta de fácil implementação e que permitiu prever o caminho do processo de sucessão para o povoamento todo e para os grupos ecológicos de espécies, ampliando o entendimento dos mecanismos que regem o comportamento

  11. Efeito de 6-benzilaminopurina sobre a propagação in vitro de Mimosa caesalpiniifolia Benth. (Fabaceae

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    Rafaela Maria de França Bezerra

    2014-10-01

    Full Text Available A Mimosa caesalpiniifolia, popularmente conhecida como sabiá, é uma espécie nativa da Caatinga que, em razão de suas inúmeras potencialidades, tem enfrentado um processo de exaustiva exploração, tornando iminente a necessidade de se utilizar alternativas sustentáveis que permitam a sua reposição em ambiente natural e a conservação de seu genótipo. A micropropagação tem sido considerada técnica promissora nesse sentido, pois viabiliza a produção de mudas em larga escala e com elevada sanidade. Citocininas como a 6-benzilaminopurina (BAP são importantes nesse processo, pois influenciam consideravelmente o crescimento e a morfogênese in vitro e permitem a formação de bancos de germoplasma in vitro. O objetivo deste trabalho foi verificar a influência de concentrações de BAP sobre a indução de brotações in vitro em M. caesalpiniifolia. Segmentos cotiledonares obtidos de plântulas germinadas in vitro foram inoculados em meio de cultura WPM suplementados com seis concentrações de BAP: 0,0; 4,44; 8,88; 17,76; 26,64; e 35,52 µmol/L. O delineamento experimental utilizado foi o inteiramente casualizado com seis tratamentos, três repetições e cada repetição composta por 10 unidades experimentais. Aos 30 dias, foram avaliados o número de explantes responsivos, o número de brotos por explante e a presença de calos, oxidação e contaminação. Os dados foram avaliados estatisticamente através da Análise de Correspondência e mediante o ajuste de Equações de Regressão. Verificou-se que a concentração de 17,76 µmol/L apresentou-se mais responsiva em relação à taxa de multiplicação e ao número de brotações, sendo, portanto, a concentração mais indicada para a propagação in vitro de M. caesalpiniifolia.

  12. Abordagem fitoquímica, composição bromatológica e atividade antibacteriana de Mimosa tenuiflora (Wild Poiret E Piptadenia stipulacea (Benth Ducke - doi: 10.4025/actascibiolsci.v33i1.5366 Phytochemical approach, bromatologic composition and antibacterial activity of Mimosa tenuiflora (Wild Poiret and Piptadenia stipulacea (Benth Ducke - doi: 10.4025/actascibiolsci.v33i1.5366

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    Onaldo Guedes Rodrigues

    2011-02-01

    Full Text Available A necessidade de encontrar novas drogas eficazes no combate microbiano tem aumentado a cada dia e estimulado a busca de novos compostos naturais com atividades biológicas. Neste trabalho, realizaram-se estudo fitoquímico e análises microbiológicas com os extratos etanólicos das espécies (jurema-preta Mimosa tenuiflora (Wild Poiret e (jurema-branca Piptadenia stipulacea (Benth Ducke, frente a linhagens de bactérias patogênicas. O pó da casca do caule de ambas as espécies foi submetido à avaliação bromatológica e determinados os teores de Matéria Seca, Matéria Mineral, Proteína Bruta, Fibra em Detergente Neutro e Energia Bruta. Os resultados para a prospecção química indicaram a presença de taninos e outros compostos fenólicos, bem como a presença de saponinas em ambos os extratos. Os extratos das duas espécies demonstraram que mais de uma parte das plantas possui atividade antimicrobiana. A composição bromatológica da casca do caule de jurema-preta e jurema-branca apresentou teores diferenciados para as variáveis avaliadas.The need to find new efficient drugs to combat microbes has increased the search for new natural compounds with biological activities. In this work, phytochemical studies and microbiological analysis were carried out with the ethanol extracts of Mimosa tenuiflora (Wild Poiret and Piptadenia stipulacea (Benth Ducke on pathogenic bacteria strains. The bark powders of both species were submitted to bromatologic evaluation and the levels of dry matter, mineral matter, crude protein, neutral detergent fiber (NDF and crude energy were determined. The results of the chemical search chemical showed the presence of tannins and other phenolic compounds as well as the presence of saponins in both extracts. The microbiologic evaluation of the extracts of both species showed that more than one part of the plants had antimicrobial activity. The bromatologic composition of the bark powder of Mimosa tenuiflora (Wild

  13. Deposição e acúmulo de matéria seca e nutrientes em serapilheira em um bosque de sabiá (Mimosa caesalpiniifolia Benth. Litter dry mass and nutrient deposition and accumulation in a sabiá (Mimosa caesalpiniifolia Benth. woodlot

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    Rinaldo Luiz Caraciolo Ferreira

    2007-02-01

    Full Text Available A quantificação da deposição de serapilheira e o acúmulo de manta orgânica são etapas importantes dos estudos de ciclagem de nutrientes. Com esse fim, objetivou-se quantificar a deposição de serapilheira e o acúmulo de manta orgânica em um povoamento de Sabiá (Mimosa caesalpiniifolia Benth., no campo experimental pertencente à Empresa Pernambucana de Pesquisa Agropecuária - IPA, Itambé, PE. Para quantificação da serapilheira foram utilizados 20 coletores de madeira de 0,50 x 0,50 x 15 cm, à distância do solo de 30 cm, distribuídos sistematicamente na área. O material depositado foi coletado mensalmente no período de outubro/2000 a setembro/2001. O material colhido em cada coletor foi separado em folhas, galhos, flores, frutos e miscelânea. A manta orgânica não decomposta sobre o solo foi amostrada, ao acaso, nas proximidades da caixa coletora, obtendo-se 20 amostras mensais. De cada amostra foi retirado o material contido numa área de 30 x 30 cm. O material colhido foi separado em folhas, flores, frutos, miscelânea e galhos. As amostras foram levadas à estufa a 60 º C no período de 48 horas até peso constante. A deposição mensal de serapilheira e frações folha e legume ocorreu em outubro, provavelmente devido ao início do período seco. A deposição e o estoque de serapilheira foram estimados em 7830,44 kg.ha-1.ano-1 e 8906.9, kg.ha-1, respectivamente, com a fração folha dominando. A fração folha apresentou os maiores teores de nutrientes.Quantification of litter deposition and organic mantle accumulation are important steps on nutrient cycling studies. This study aimed to quantify litter deposition and organic mantle in a sabiá (Mimosa caesalpiniifolia Benth woodlot in an experimental farm in the Brazilian northeast region. Twenty 0.50 x 0.50 x 0.15 m wood collectors, to quantify litter deposition, were systematically distributed over the area elevated 30 cm above the soil. Deposited material was

  14. Composição química de forrageiras e seletividade de bovinos em bosque-de-sabiá (Mimosa caesalpiniifolia Benth. nos períodos chuvoso e seco Chemical composition of forage and selectivity by bovines of "sabiá" (Mimosa caesalpiniaefolia Benth. in the rainy and dry seasons

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    Ednéia de Lucena Vieira

    2005-10-01

    Full Text Available A pesquisa foi desenvolvida no período de junho de 1999 a março de 2000, com o objetivo de determinar, nos períodos chuvoso e seco, a composição química de sabiá (Mimosa caesalpiniifolia Benth., com acúleo e sem acúleo, na dieta de bovinos em condições de pastejo e a composição botânica do bosque de sabiá e da dieta selecionada. Não foi observada diferença significativa quanto à composição química entre os sabiás com e sem acúleo, obtendo-se valores médios, no período chuvoso, de 26,45% de matéria seca (MS, 27,63% de proteína bruta (PB, 44,39% de fibra em detergente neutro (FDN, 25,80% de fibra em detergente ácido (FDA, 1,24% de cálcio (Ca, 0,22% de fósforo (P, 1,63% de potássio (K e 1,12% de sódio (Na. No período seco, os valores médios observados foram de 42,39% de MS; 19,30% de PB; 39,05% de FDN; 21,83% de FDA; 1,25% de Ca; 0,15% de P; 1,63% de K e 0,27% de Na. Para a composição botânica da dieta, observou-se que, durante o período chuvoso, as amostras de extrusa apresentaram maior participação de sabiá (83,1% que no período seco ( 12,3%. Os resultados obtidos indicaram que, durante o período chuvoso, os animais selecionaram sabiá em maior quantidade para sua dieta e que a coleta manual, como realizada, superestimou a fração fibrosa da dieta calculada a partir da composição química da extrusa.This trial was carried out from June 1999 to March 2000 to evaluate the chemical composition of samples of sabiá legume (Mimosa caesalpiniaefolia Benth., with and without thorns, collected in the rainy and dry seasons and to compare the botanical composition of sabiá pasture with that of the diet selected by the grazing animals. No significant difference was observed in chemical composition between samples of sabiá with and without thorns. The mean values obtained in the rainy samples were: 26.45% drymatter (DM, 27.63% crude protein (CP, 44.39% neutral detergent fiber (NDF, 25.80% acid detergent fiber

  15. Efeito alelopático do extrato aquoso de sabiá na germinação de sementes de fava Allelopathic effect of aqueous extract of Mimosa caesalpiniaefolia Benth. in seed germination of Phaseolus lunatus

    Directory of Open Access Journals (Sweden)

    Elane Grazielle Borba de Sousa Ferreira

    2010-09-01

    Full Text Available O sabiá (Mimosa caesalpiniaefolia Benth. é uma planta medicinal que pode ser utilizada em sistemas agroflorestais. É empregada na composição de pastagens arbóreas, em faixas entre plantações, para enriquecer capoeiras e ainda pode ser empregada como cerca viva. A fava (Phaseolus lunatus L. é uma das quatro espécies do gênero Phaseolus exploradas comercialmente, seu consumo é preferencialmente na forma de grãos verdes cozidos ou na forma de conserva. O presente trabalho teve como objetivo verificar o efeito alelopático do extrato aquoso do sabiá sobre a germinação de sementes e crescimento inicial de plântulas de fava. As sementes de fava foram postas para germinar em caixas plásticas, onde foram semeadas entre o substrato vermiculita, e em seguida colocados em germinador a 25 ºC e sob luz contínua. O substrato foi umedecido, com o extrato aquoso de folhas jovens de sabiá, nas concentrações de 25; 50; 75 e 100%, além da testemunha umedecida apenas com água destilada. As variáveis avaliadas foram: porcentagem, primeira contagem e índice de velocidade de germinação além do comprimento da raiz primária. O delineamento experimental utilizado foi o inteiramente casualizado, com quatro repetições de 25 sementes cada. Os dados obtidos foram submetidos à análise de variância e de regressão polinomial. No estudo da regressão polinomial foi empregada a equação que melhor se ajustou aos dados. Os valores em porcentagem foram transformados em arc sen (n/1000,5. Conclui-se que as diferentes concentrações do extrato de folhas jovens de sabiá utilizadas não prejudicaram a germinação das sementes de fava.The Mimosa caesalpiniaefolia Benth. is a medicinal plant that can be used in agroforestry systems, is also employed in the composition of pasture trees in strips between fields, to enrich brush fields and as a hedge. The Phaseolus lunatus L. is one of four species of the genus Phaseolus exploited commercially; its

  16. Two new species of Lopesia Rübsaamen (Diptera, Cecidomyiidae) associated with Mimosa hostilis (Mimosaceae) in Brazil

    OpenAIRE

    Valéria Cid Maia; G. Wilson Fernandes; Henrique Magalhães; Jean Carlos Santos

    2010-01-01

    Two new species of Lopesia Rübsaamen (Diptera, Cecidomyiidae) associated with Mimosa hostilis (Mimosaceae) in Brazil Two new species of Lopesia Rübsaamen (Diptera, Cecidomyiidae, Lopesiini), L. mimosae Maia, sp. nov. and L. pernambucensis Maia, sp. nov., that induce galls on Mimosa hostilis Benth. (Mimosaceae) are described and illustrated based on material from "caatinga" in Pernambuco, Brazil.

  17. Two new species of Lopesia Rübsaamen (Diptera, Cecidomyiidae associated with Mimosa hostilis (Mimosaceae in Brazil

    Directory of Open Access Journals (Sweden)

    Valéria Cid Maia

    2010-01-01

    Full Text Available Two new species of Lopesia Rübsaamen (Diptera, Cecidomyiidae associated with Mimosa hostilis (Mimosaceae in Brazil Two new species of Lopesia Rübsaamen (Diptera, Cecidomyiidae, Lopesiini, L. mimosae Maia, sp. nov. and L. pernambucensis Maia, sp. nov., that induce galls on Mimosa hostilis Benth. (Mimosaceae are described and illustrated based on material from "caatinga" in Pernambuco, Brazil.

  18. Aporte e decomposição de serapilheira em áreas de floresta secundária, plantio de sabiá (Mimosa caesalpiniaefolia Benth. e andiroba (Carapa guianensis Aubl. na Flona Mário Xavier, RJ.

    Directory of Open Access Journals (Sweden)

    Milton Marques Fernandes

    2010-08-01

    Full Text Available Este trabalho teve como objetivo avaliar o aporte e a decomposição da serapilheira em áreas de floresta secundária espontânea, plantio de sabiá (Mimosa caesalpiniaefolia Benth. e plantio de andiroba (Carapa guianensis Aubl.. Para avaliação do aporte de serapilheira, foram instalados dez coletores cônicos sendo o aporte avaliado durante o período de outubro de 2003 a setembro de 2004. Posteirormente, foi feita a quantificação dos macronutrientes (N, P e K. A avaliação da taxa de decomposição foi realizada utilizando-se o método dos litter bags. A área de plantio de andiroba aportou a maior quantidade de serrapilheira 9,20 Mg ha-1, sendo seguida pelo plantio de sabiá com 9,06 Mg ha-1 e pela floresta secundária espontânea 7,63 Mg ha-1 por ano. A serapilheira das áreas de plantio de sabiá e do plantio de andiroba apresentou maiores valores de nitrogênio e fósforo que a área de floresta secundária. A velocidade de decomposição da serapilheira foi maior nas áreas de plantio de sabiá e andiroba, podendo tal comportamento ter sido influenciado pelo maior conteúdo de nitrogênio na serapilheira. O fósforo foi o nutriente que apresentou maior velocidade de liberação.

  19. Influence of Removal of a Non-native Tree Species Mimosa caesalpiniifolia Benth. on the Regenerating Plant Communities in a Tropical Semideciduous Forest Under Restoration in Brazil

    Science.gov (United States)

    Podadera, Diego S.; Engel, Vera L.; Parrotta, John A.; Machado, Deivid L.; Sato, Luciane M.; Durigan, Giselda

    2015-11-01

    Exotic species are used to trigger facilitation in restoration plantings, but this positive effect may not be permanent and these species may have negative effects later on. Since such species can provide a marketable product (firewood), their harvest may represent an advantageous strategy to achieve both ecological and economic benefits. In this study, we looked at the effect of removal of a non-native tree species ( Mimosa caesalpiniifolia) on the understory of a semideciduous forest undergoing restoration. We assessed two 14-year-old plantation systems (modified "taungya" agroforestry system; and mixed plantation using commercial timber and firewood tree species) established at two sites with contrasting soil properties in São Paulo state, Brazil. The experimental design included randomized blocks with split plots. The natural regeneration of woody species (height ≥0.2 m) was compared between managed (all M. caesalpiniifolia trees removed) and unmanaged plots during the first year after the intervention. The removal of M. caesalpiniifolia increased species diversity but decreased stand basal area. Nevertheless, the basal area loss was recovered after 1 year. The management treatment affected tree species regeneration differently between species groups. The results of this study suggest that removal of M. caesalpiniifolia benefited the understory and possibly accelerated the succession process. Further monitoring studies are needed to evaluate the longer term effects on stand structure and composition. The lack of negative effects of tree removal on the natural regeneration indicates that such interventions can be recommended, especially considering the expectations of economic revenues from tree harvesting in restoration plantings.

  20. Germinação de sementes de Mimosa caesalpiniifolia Benth. (sansão-do-campo sob diferentes condições de temperatura, luz e umidade. Seed germination of Mimosa caesalpiniifolia Benth. Under different conditions of temperature, light and moisture.

    Directory of Open Access Journals (Sweden)

    Márcia Balistiero FIGLIOLIA

    2008-12-01

    Full Text Available Mimosa caesalpiniifolia (Fabaceae éuma espécie arbórea característica da caatinga,nativa da região Nordeste brasileira. Neste trabalhoforam realizados dois experimentos. No primeiroexperimento foi estudado o efeito da coloração dassementes (verde e marrom e da temperatura para agerminação (25, 30 e 20-30 ºC. No segundoexperimento foi estudado o efeito do volume deágua destilada adicionado ao substrato (45, 60 e75 mL e da qualidade de luz (ausência de luz e luzdos espectros branco, vermelho e vermelho-extremo,na germinação das sementes de coloração verdesubmetidas a 30 ºC. Os testes de germinação foramconduzidos sob fotoperíodo de 8 h, utilizando 30 gde vermiculita como substrato, e tiveram a duraçãode 14 dias. Foram avaliados a porcentagem final eo índice de velocidade de germinação dassementes. Os resultados obtidos no primeiroexperimento mostraram que as sementes decoloração verde são de melhor qualidade fisiológicae que a temperatura de 30 ºC foi a mais adequadapara a germinação. Os resultados obtidos no segundoexperimento mostraram que: (a as sementes decoloração verde são fotoblásticas positivaspreferenciais; (b o teste de germinação pode serconduzido sob luz branca, em substrato umedecidocom 75 mL de água, e (c os resultados obtidospermitem supor que, em condições naturais, assementes sejam capazes de germinar tanto a plenosol (luz do espectro vermelho como sob luzfiltrada pela vegetação (espectro vermelho-extremo.Mimosa caesalpiniifolia (Fabaceae is animportant arboreal species native from the caatingavegetation of the northeastern of Brazil. In thiswork two experiments were carried out. In the firstexperiment, the effect of seed color (green andbrown and temperature for germination (25, 30,and 20-30 ºC was studied. In the second experiment,the effect of distilled water added to substrate(45, 60, and 75 mL and light quality (darkness andwhite, red, and far-red light on germination at30 ºC of

  1. Electronic Mimosa

    Science.gov (United States)

    Gupta, Sanjay; Datta, Meera S.; Rana, Vivek; Grover, Shailesh

    1996-04-01

    This paper describes the design of a low-cost, 2D, electromagnetic tracking device for personal computers. This interface makes use of the well-known principle of electromagnetic induction to locate the position of a transmitter in an x-y plane. This device has a ring which is worn by the user on the index finger. The computer monitor is overlaid with a transparent screen equipped with tuned electromagnetic sensors. These sensors pick up the signals transmitted by the transmitter coil on the finger. The receiver circuit extracts the envelope of the received signal and digitizes it. These digitized values of x and y axis signals are read by the computer through the standard parallel port. The system software running on the computer calculates the x and y co-ordinates of the transmitter coil and displays a cursor at that location. The transmitter also has a button which can be used like a mouse button. This keypress information is also transmitted by the electromagnetic means. The device driver for this tracker replaces the standard mouse driver. Hence most applications which use a mouse can also use this tracker. Its name `Mimosa' indicates that the user need not touch the screen (Mimosa Pudica is the Latin name of a plant whose leaves wilt when touched). Presently work is on to achieve uniform sensitivity over the entire screen and reducing transmitter power consumption. In order to demonstrate its working, a small, 3D game was written. The player has to reach a pre-defined location after traversing through a maze. The paper describes the interface electronics, system software, mechanical design and the sample application.

  2. The typification of Mimosa senegal

    Directory of Open Access Journals (Sweden)

    J. H. Ross

    1975-12-01

    Full Text Available Analysis of the protologue of  Mimosa senegal L. in Species Plantarum ed. I: 521 (1753 indicated that it embraced two different elements. I he absence ol a type specimen or the existence of an illustration from which Linnaeus could have drawn up his diagnostic phrase-name necessitated the selection of a neotype to preserve the application of the name  M. senegal.

  3. Biochemical and immunohistochemical characterization of Mimosa annexin.

    Science.gov (United States)

    Hoshino, Daisuke; Hayashi, Asami; Temmei, Yusuke; Kanzawa, Nobuyuki; Tsuchiya, Takahide

    2004-09-01

    To characterize the biochemical properties of plant annexin, we isolated annexin from Mimosa pudica L. and analyzed the biochemical properties conserved between Mimosa annexin and animal annexins, e.g. the ability to bind phospholipid and F-actin in the presence of calcium. We show that Mimosa annexin is distributed in a wide variety of tissues. Immunoblot analysis also revealed that the amount of annexin is developmentally regulated. To identify novel functions of Mimosa annexin, we examined the pattern of distribution and the regulation of its expression in the pulvinus. The amount of annexin in the pulvinus increased at night and was sensitive to abscisic acid; however, there was no detectable induction of annexin by cold or mechanical stimulus. Annexin distribution in the cell periphery during the daytime was changed to a cytoplasmic distribution at night, indicating that Mimosa annexin may contribute to the nyctinastic movement in the pulvinus.

  4. Mimosa pudica, Dionaea muscipula and anesthetics.

    Science.gov (United States)

    De Luccia, Thiago Paes de Barros

    2012-09-01

    Some studies showed that anesthetics reduce the response of physical stimuli in Mimosa pudica and in Venus Flytrap (Dionaea muscipula), peculiar plants that have the ability to respond to touch stimuli. In this research we tested the effects of ketamine, lidocaine, diethyl ether, and amlodipine on the movements of Mimosa pudica and Venus Flytrap. With a literature review, we tried to bring elements to theorize about the interaction of these substances with these plants. The angular displacement in Mimosa´s petiole and in Dionaea leaves is what was measured to compare the drugs group with control groups.

  5. Boolean function applied to Mimosa pudica movements.

    Science.gov (United States)

    De Luccia, Thiago Paes de Barros; Friedman, Pedro

    2011-09-01

    Seismonastic or thigmonastic movements of Mimosa pudica L. is mostly because of the fast loss of water from swollen motor cells, resulting in temporary collapse of cells and quick curvature in the parts where these cells are located. Because of this, the plant has been much studied since the 18th century, leading us to think about the classical binomial stimulus-response (action-reaction) when compared to animals. Mechanic and electrical stimuli were used to investigate the analogy of mimosa branch with an artificial neuron model and to observe the action potential propagation through the mimosa branch. Boolean function applied to the mimosa branch in analogy with an artificial neuron model is one of the peculiarities of our hypothesis.

  6. Mimosa 32-ter Chip Characterisation

    CERN Document Server

    Behera, Arabinda

    2013-01-01

    The Inner Tracking System (ITS) is a very important part of the ALICE detector. Present ITS is made up of 6 coaxial layers of silicon detectors (2 SPD, 2 SDD and 2 SSD). This system has a lot of limitations. Its tracking efficiency and resolution is low. Its readout rate is also very slow. Its not possible to study charm and beauty baryons and mesons with this system. So the upcoming ITS Upgrade plan will try to overcome these shortcomings. A very crucial part in this plan is to replace the existing hybrid sensors by new and advanced Monolithic Active Pixel Sensors(MAPS). In MAPS the sensor and the electronics are embedded in the same chip. The MAPS will reduce the material budget, increase the readout rate and enhance the tracking efficiency and momentum resolution. In this report I will present the results for the Characterisation of MIMOSA 32-ter chip, which is a MAPS. My main aim is to calculate the Charge Collection Efficiency of different sectors of the chip and compare between them. And most importa...

  7. Triterpenos de Hyptis fasciculata Benth.

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    D.Q. Falcão

    2003-01-01

    Full Text Available Partes aéreas do arbusto Hyptis fasciculata Benth. foram estudadas do ponto de vista químico. Após vários processos cromatográficos em coluna de gel de sílica, foi possível isolar um triterpeno livre (ácido betulínico, além de triterpenos em mistura (ácido ursólico com ácido betulínico e ácido ursólico com ácido oleanólico, esteróides em mistura (sitosterol e estigmasterol e misturas de estigmasterol com ácido caféico e sitosterol com lignana. Tais substâncias foram identificadas por ressonância magnética nuclear de 1H e 13C.The species Hyptis fasciculata Benth. is a shrub that is still unknown by the chemical point of view. This species has been studied in our group using their aerial parts. After many cromatographic processes over silica gel column, it was possible to isolate free triterpene (betulinic acid and triterpenes in mixture (ursolic acid with betulinic acid and ursolic acid with oleanolic acid, one steroid mixture (sitosterol and stigmasterol and a mixture of steroid with cafeic acid (stigmasterol and cafeic acid and with lignan (sitosterol and sesamin. These compounds were identified by nuclear magnetic ressonance 1H and 13C.

  8. Anticonvulsant activity of Mimosa pudica decoction.

    Science.gov (United States)

    Ngo Bum, E; Dawack, D L; Schmutz, M; Rakotonirina, A; Rakotonirina, S V; Portet, C; Jeker, A; Olpe, H-R; Herrling, P

    2004-06-01

    The decoction of Mimosa pudica leaves given intraperitoneally at dose of 1000-4000 mg/kg protected mice against pentylentetrazol and strychnine-induced seizures. M. pudica had no effect against picrotoxin-induced seizures It also antagonized N-methyl-D-aspartate- induced turning behavior. These properties could explain its use in African traditional medicine. Copyright 2004 Elsevier B.V.

  9. Memory elements in the electrical network of Mimosa pudica L

    National Research Council Canada - National Science Library

    Volkov, Alexander G; Reedus, Jada; Mitchell, Colee M; Tuckett, Clayton; Volkova, Maya I; Markin, Vladislav S; Chua, Leon

    2014-01-01

    .... Here we found that memristors exist in vivo. The electrostimulation of the Mimosa pudica by bipolar sinusoidal or triangle periodic waves induce electrical responses with fingerprints of memristors...

  10. Molecular electronics in pinnae of Mimosa pudica.

    Science.gov (United States)

    Volkov, Alexander G; Foster, Justin C; Markin, Vladislav S

    2010-07-01

    Bioelectrochemical circuits operate in all plants including the sensitive plant Mimosa pudica Linn. The activation of biologically closed circuits with voltage gated ion channels can lead to various mechanical, hydrodynamical, physiological, biochemical, and biophysical responses. Here the biologically closed electrochemical circuit in pinnae of Mimosa pudica is analyzed using the charged capacitor method for electrostimulation at different voltages. Also the equivalent electrical scheme of electrical signal transduction inside the plant's pinna is evaluated. These circuits remain linear at small potentials not exceeding 0.5 V. At higher potentials the circuits become strongly non-linear pointing to the opening of ion channels in plant tissues. Changing the polarity of electrodes leads to a strong rectification effect and to different kinetics of a capacitor. These effects can be caused by a redistribution of K(+), Cl(-), Ca(2+), and H(+) ions through voltage gated ion channels. The electrical properties of Mimosa pudica were investigated and equivalent electrical circuits within the pinnae were proposed to explain the experimental data.

  11. Ionic Liquid-Based Microwave-Assisted Extraction of Flavonoids from Bauhinia championii (Benth. Benth.

    Directory of Open Access Journals (Sweden)

    Wei Xu

    2012-12-01

    Full Text Available An ionic liquids (IL-based microwave-assisted approach for extraction and determination of flavonoids from Bauhinia championii (Benth. Benth. was proposed for the first time. Several ILs with different cations and anions and the microwave-assisted extraction (MAE conditions, including sample particle size, extraction time and liquid-solid ratio, were investigated. Two M 1-butyl-3-methylimidazolium bromide ([bmim] Br solution with 0.80 M HCl was selected as the optimal solvent. Meanwhile the optimized conditions a ratio of liquid to material of 30:1, and the extraction for 10 min at 70 °C. Compared with conventional heat-reflux extraction (CHRE and the regular MAE, IL-MAE exhibited a higher extraction yield and shorter extraction time (from 1.5 h to 10 min. The optimized extraction samples were analysed by LC-MS/MS. IL extracts of Bauhinia championii (Benth.Benth consisted mainly of flavonoids, among which myricetin, quercetin and kaempferol, β-sitosterol, triacontane and hexacontane were identified. The study indicated that IL-MAE was an efficient and rapid method with simple sample preparation. LC-MS/MS was also used to determine the chemical composition of the ethyl acetate/MAE extract of Bauhinia championii (Benth. Benth, and it maybe become a rapid method to determine the composition of new plant extracts.

  12. Effect of iron deficiency stress on leaves movements and electrical potentials in mimosa (Mimosa pudica L.

    Directory of Open Access Journals (Sweden)

    Edward Ślesak

    2014-01-01

    Full Text Available The aim of the studies was to trace the motorical and electrical activity of the mimosa (Mimosa pudica L. grown under conditions of iron deficiency. The speed of leaf folding was measured (motorical activity and the action potential induced with thermic and light stimuli and turgorin (electrical activity was recorded. It was found that the iron deficiency caused acidification of medium and the maximum of the process coincided with the period when the young leaves were turning green. Chlorotic mimosa leaves, not detached from the plant, showed an increased motorical activity. Motorical and electrical activity of the leaves were inhibited by an inhibitor of the plasmalemma redox systems - quinacrine, and stimulated by blue light. Leaf movement factor - turgorin - caused a hypersensitivity of chlorotic plants. It follows from the studies that the observed effects resulted from the adaptation of mimosa to the iron stress. The adaptation was a result of formation of new plasmalemma redox systems (turbo-reductase, responsible for maintaining high energy levels in the cells.

  13. Production and photosynthetic activity of Mimosa Verde and Mimosa Roxa lettuce in two farming systems

    Directory of Open Access Journals (Sweden)

    Aline Mabel Rosa

    2014-08-01

    Full Text Available Lettuce (Lactuca sativa L. is the most commonly consumed leaf vegetable in the Brazilian diet, and it is a good source of vitamins and minerals. It is widely grown in the conventional farming system. However, the hydroponic farming system has been gaining importance in the market, wining confidence from consumers, who are becoming increasingly more demanding on food quality. The objective of this study was to evaluate the performance of two lettuce cultivars on hydroponic and conventional farming systems for the production of fresh mass (FM and dry mass (DM, photosynthesis, contents of chlorophyll and anthocyanin. The following two experiments were carried out: hydroponics farming (HF and conventional farming (CF, performed in protect and unprotect environments, respectively, in Florianópolis, SC. Mimosa Verde cultivar (MV showed greater fresh mass than Mimosa Roxa (MR, in both farming systems and the two cultivars presented better performance in the hydroponic system (287.7 g MV and 139.1 g MR than the conventional system (129.7 g MV and 111.8 g MR. Mimosa Verde cultivar presented lower average contents of total chlorophyll (7.7 mg g-¹ FM than Mimosa Roxa (11.8 mg g-¹FM, and both cultivars displayed higher means for this variable in the hydroponic farming system. Mimosa Roxa presented higher contents of anthocyanin in the conventional system (88.24 mg g-¹ FM than the ones in the hydroponic system (36.89 mg g-¹ FM. The best results for CO2 net assimilation rate regarded to photosyntheticaly active photon flux density were found in the hydroponic system, for both lettuce cultivars. Variation in the contents of chlorophyll were also found. Those variations were higher in the protected system than in the hydroponic system and contents of anthocyanin were higher in the conventional system.

  14. Antibacterial efficacy of Mimosa Pudica (Lajavanti against streptococcus mutans

    Directory of Open Access Journals (Sweden)

    Swati V Balsaraf

    2014-01-01

    Full Text Available Introduction: The Mimosa pudica plant also known as "touch me not" plant, "shame" plant or laajvanti is a widely grown perennial herb as a show plant because of the mysterious action of its leaves which closes when touched. Mimosa pudica is also known for various medicinal purposes such as treatment of depression, piles, insomnia and many more. It has also been used for dental purposes. Literature shows evidence of its use for dental decay, bleeding gums throughout India and worldwide. Materials and Methods: Mimosa pudica plant whole aqueous extract in 10%, 20% and 5% concentration, chlorhexidine mouth wash as control, Streptococcus mutans (Microbial Type Culture Collection. Using agar well method the efficacy of Mimosa pudica extract was tested. Results: The plant extract couldn′t show efficacy as the microorganisms didn′t show any growth. Conclusion: The potential of this drug needs further investigation.

  15. MimoSA: a system for minimotif annotation

    Directory of Open Access Journals (Sweden)

    Kundeti Vamsi

    2010-06-01

    Full Text Available Abstract Background Minimotifs are short peptide sequences within one protein, which are recognized by other proteins or molecules. While there are now several minimotif databases, they are incomplete. There are reports of many minimotifs in the primary literature, which have yet to be annotated, while entirely novel minimotifs continue to be published on a weekly basis. Our recently proposed function and sequence syntax for minimotifs enables us to build a general tool that will facilitate structured annotation and management of minimotif data from the biomedical literature. Results We have built the MimoSA application for minimotif annotation. The application supports management of the Minimotif Miner database, literature tracking, and annotation of new minimotifs. MimoSA enables the visualization, organization, selection and editing functions of minimotifs and their attributes in the MnM database. For the literature components, Mimosa provides paper status tracking and scoring of papers for annotation through a freely available machine learning approach, which is based on word correlation. The paper scoring algorithm is also available as a separate program, TextMine. Form-driven annotation of minimotif attributes enables entry of new minimotifs into the MnM database. Several supporting features increase the efficiency of annotation. The layered architecture of MimoSA allows for extensibility by separating the functions of paper scoring, minimotif visualization, and database management. MimoSA is readily adaptable to other annotation efforts that manually curate literature into a MySQL database. Conclusions MimoSA is an extensible application that facilitates minimotif annotation and integrates with the Minimotif Miner database. We have built MimoSA as an application that integrates dynamic abstract scoring with a high performance relational model of minimotif syntax. MimoSA's TextMine, an efficient paper-scoring algorithm, can be used to

  16. Learning in Plants: Lessons from Mimosa pudica

    Science.gov (United States)

    Abramson, Charles I.; Chicas-Mosier, Ana M.

    2016-01-01

    This article provides an overview of the early Mimosa pudica literature; much of which is in journals not easily accessible to the reader. In contrast to the contemporary plant learning literature which is conducted primarily by plant biologists, this early literature was conducted by comparative psychologists whose goal was to search for the generality of learning phenomena such as habituation, and classical conditioning using experimental designs based on animal conditioning studies. In addition to reviewing the early literature, we hope to encourage collaborations between plant biologists and comparative psychologists by familiarizing the reader with issues in the study of learning faced by those working with animals. These issues include no consistent definition of learning phenomena and an overreliance on the use of cognition. We suggested that greater collaborative efforts be made between plant biologists and comparative psychologists if the study of plant learning is to be fully intergraded into the mainstream behavior theory. PMID:27065905

  17. Mimosa pudica L. (Laajvanti): An overview

    Science.gov (United States)

    Ahmad, Hafsa; Sehgal, Sakshi; Mishra, Anurag; Gupta, Rajiv

    2012-01-01

    Mimosa pudica L. (Mimosaceae) also referred to as touch me not, live and die, shame plant and humble plant is a prostrate or semi-erect subshrub of tropical America and Australia, also found in India heavily armed with recurved thorns and having sensitive soft grey green leaflets that fold and droop at night or when touched and cooled. These unique bending movements have earned it a status of ‘curiosity plant’. It appears to be a promising herbal candidate to undergo further exploration as evident from its pharmacological profile. It majorly possesses antibacterial, antivenom, antifertility, anticonvulsant, antidepressant, aphrodisiac, and various other pharmacological activities. The herb has been used traditionally for ages, in the treatment of urogenital disorders, piles, dysentery, sinus, and also applied on wounds. This work is an attempt to explore and compile the different pharmacognostic aspects of the action plant M. pudica reported till date. PMID:23055637

  18. Mimosa pudica L. (Laajvanti: An overview

    Directory of Open Access Journals (Sweden)

    Hafsa Ahmad

    2012-01-01

    Full Text Available Mimosa pudica L. (Mimosaceae also referred to as touch me not, live and die, shame plant and humble plant is a prostrate or semi-erect subshrub of tropical America and Australia, also found in India heavily armed with recurved thorns and having sensitive soft grey green leaflets that fold and droop at night or when touched and cooled. These unique bending movements have earned it a status of ′curiosity plant′. It appears to be a promising herbal candidate to undergo further exploration as evident from its pharmacological profile. It majorly possesses antibacterial, antivenom, antifertility, anticonvulsant, antidepressant, aphrodisiac, and various other pharmacological activities. The herb has been used traditionally for ages, in the treatment of urogenital disorders, piles, dysentery, sinus, and also applied on wounds. This work is an attempt to explore and compile the different pharmacognostic aspects of the action plant M. pudica reported till date.

  19. Mimosa pudica L. (Laajvanti): An overview.

    Science.gov (United States)

    Ahmad, Hafsa; Sehgal, Sakshi; Mishra, Anurag; Gupta, Rajiv

    2012-07-01

    Mimosa pudica L. (Mimosaceae) also referred to as touch me not, live and die, shame plant and humble plant is a prostrate or semi-erect subshrub of tropical America and Australia, also found in India heavily armed with recurved thorns and having sensitive soft grey green leaflets that fold and droop at night or when touched and cooled. These unique bending movements have earned it a status of 'curiosity plant'. It appears to be a promising herbal candidate to undergo further exploration as evident from its pharmacological profile. It majorly possesses antibacterial, antivenom, antifertility, anticonvulsant, antidepressant, aphrodisiac, and various other pharmacological activities. The herb has been used traditionally for ages, in the treatment of urogenital disorders, piles, dysentery, sinus, and also applied on wounds. This work is an attempt to explore and compile the different pharmacognostic aspects of the action plant M. pudica reported till date.

  20. Learning in Plants: Lessons from Mimosa pudica

    Directory of Open Access Journals (Sweden)

    Charles Ira Abramson

    2016-03-01

    Full Text Available This article provides an overview of the early Mimosa pudica literature; much of which is in journals not easily accessible to the reader. In contrast to the contemporary plant learning literature which is conducted primarily by plant biologists, this early literature was conducted by comparative psychologists whose goal was to search for the generality of learning phenomena such as habituation, and classical conditioning using experimental designs based on animal conditioning studies. In addition to reviewing the early literature, we hope to encourage collaborations between plant biologists and comparative psychologists by familiarizing the reader with issues in the study of learning faced by those working with animals. These issues include no consistent definition of learning phenomena and an overreliance on the use of cognition. We suggested that greater collaborative efforts be made between plant biologists and comparative psychologists if the study of plant learning is to be fully intergraded into the mainstream behavior theory.

  1. Learning in Plants: Lessons from Mimosa pudica.

    Science.gov (United States)

    Abramson, Charles I; Chicas-Mosier, Ana M

    2016-01-01

    This article provides an overview of the early Mimosa pudica literature; much of which is in journals not easily accessible to the reader. In contrast to the contemporary plant learning literature which is conducted primarily by plant biologists, this early literature was conducted by comparative psychologists whose goal was to search for the generality of learning phenomena such as habituation, and classical conditioning using experimental designs based on animal conditioning studies. In addition to reviewing the early literature, we hope to encourage collaborations between plant biologists and comparative psychologists by familiarizing the reader with issues in the study of learning faced by those working with animals. These issues include no consistent definition of learning phenomena and an overreliance on the use of cognition. We suggested that greater collaborative efforts be made between plant biologists and comparative psychologists if the study of plant learning is to be fully intergraded into the mainstream behavior theory.

  2. Bukholderia strains promote Mimosa spp. growth but not Macroptilium atropurpureum

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    Kaliane Sírio Araújo

    Full Text Available ABSTRACT The aim of this study was to evaluate the relationship and symbiotic efficiency of 14 strains of Burkholderia isolated from rupestrian grasslands, using M. atropurpureum and Mimosa tenuiflora as trap plants, with the species M. atropurpureum, Mimosa bimucronata and M. foliolosa. For the nodulation and symbiotic efficiency test in M. atropurpureum, long-neck bottles containing nutrient solution were used. The experiments with Mimosa spp. were carried out in tubes containing vermiculite (160 cm3 and sand (80 cm3 (2:1. The parameters under evaluation were number of nodules, nodules dry matter production, shoots dry matter, roots dry matter, and total dry matter production for all the species analyzed; and plant height, diameter, and the Dickson quality index for Mimosa species. Of the 14 tested strains, two nodulated M. atropurpureum; however, they were ineffective in promoting plant growth. All the tested strains established symbiosis with M. bimucronata, and 12 strains nodulated M. foliolosa. Of these, six promoted growth in M. bimucronata, and seven presented symbiotic efficiency in M. foliolosa. The strains UFLA 01-739, UFLA 01-748 and UFLA 01-751, isolated from M. tenuiflora, and UFLA 04-260 and UFLA 04-405, isolated from M. atropurpureum, stood out as potential inoculants for the Mimosa species evaluated in this study.

  3. Mechanical and electrical anisotropy in Mimosa pudica pulvini.

    Science.gov (United States)

    Volkov, Alexander G; Foster, Justin C; Baker, Kara D; Markin, Vladislav S

    2010-10-01

    Thigmonastic or seismonastic movements in Mimosa pudica, such as the response to touch, appear to be regulated by electrical, hydrodynamical, and chemical signal transduction. The pulvinus of Mimosa pudica shows elastic properties, and we found that electrically or mechanically induced movements of the petiole were accompanied by a change of the pulvinus shape. As the petiole falls, the volume of the lower part of the pulvinus decreases and the volume of the upper part increases due to the redistribution of water between the upper and lower parts of the pulvinus. This hydroelastic process is reversible. During the relaxation of the petiole, the volume of the lower part of the pulvinus increases and the volume of the upper part decreases. Redistribution of ions between the upper and lower parts of a pulvinus causes fast transport of water through aquaporins and causes a fast change in the volume of the motor cells. Here, the biologically closed electrochemical circuits in electrically and mechanically anisotropic pulvini of Mimosa pudica are analyzed using the charged capacitor method for electrostimulation at different voltages. Changing the polarity of electrodes leads to a strong rectification effect in a pulvinus and to different kinetics of a capacitor discharge if the applied initial voltage is 0.5 V or higher. The electrical properties of Mimosa pudica's pulvini were investigated and the equivalent electrical circuit within the pulvinus was proposed to explain the experimental data. The detailed mechanism of seismonastic movements in Mimosa pudica is discussed. © 2010 Landes Bioscience

  4. Hypolipidemic Activity of Chloroform Extract of Mimosa pudica Leaves.

    Science.gov (United States)

    Rajendran, Rekha; Krishnakumar, Ekambaram

    2010-10-01

    Mimosa pudica Lin., known as chue Mue, is a stout straggling prostrate shrubby plant, with spinous stipules and globose pinkish flower heads, and grows as weed in almost all parts of the country. It is traditionally used for its various properties and hence in the present study, chloroform extract of Mimosapudica leaves has been screened for its hypolipidemic activity. Hypolipidemic activity is screened by inducing hyperlipidemia with the help of atherogenic diet in wistar albino rats and serum levels of various biochemical parameters such as total cholesterol, triglycerides, LDL, VLDL and HDL cholesterol were determined. Atherogenic index shows the measure of the athero-genic potential of the drugs. Chloroform extract showed significant (p Mimosa pudica, may be responsible for the significant hypolipidemic activity and the results justify the use of Mimosa pudica as a significant hypolipidemic agent.

  5. Structural investigation of Mimosa pudica Linn fibre

    Science.gov (United States)

    Patra, S. R.; Pattojoshi, P.; Tiwari, T. N.; Mallick, B.

    2016-12-01

    Sensitive plant (Mimosa pudica Linn.) fibre is a natural fibre with electrically conductive property. Because of its electro-active sensing nature, it has been found very interesting among physicists, chemists, biologists, material scientists and technologists. So far as our knowledge is concerned; there is no report on the X-ray structure of M. pudica fibre using diffraction technique. In the present report, the M. pudica fibre has been extracted from the stem of the herb by sinking the stem in 10% NaOH solution for one week. The diffraction pattern of the fibre is found out to be cellulose-I. The effect of the fibre structure and its orientation due to different mounting have been investigated using X-ray diffraction technique. The I max of cellulose-I has been observed along (002) and (10overline{1)} for the perpendicular and parallel mounting of the native-fibre, respectively. Full width at half maxima of the diffraction profile turns out to be decreased with fibre orientation. Dimension of crystallite size D hkl estimated in the perpendicular mounting D_{hkl}^{ bot } is more as compared to that of the parallel mounting D_{hkl}^{{^{allel } }} . The smallest crystallite sizes observed in both parallel and perpendicular mounting are 18.78 and 30.78 Å respectively. It is expected that the present study may help to analyse the X-ray diffraction of fibre materials in general and natural fibres in particular.

  6. Structural investigation of Mimosa pudica Linn fibre

    Science.gov (United States)

    Patra, S. R.; Pattojoshi, P.; Tiwari, T. N.; Mallick, B.

    2017-04-01

    Sensitive plant ( Mimosa pudica Linn.) fibre is a natural fibre with electrically conductive property. Because of its electro-active sensing nature, it has been found very interesting among physicists, chemists, biologists, material scientists and technologists. So far as our knowledge is concerned; there is no report on the X-ray structure of M. pudica fibre using diffraction technique. In the present report, the M. pudica fibre has been extracted from the stem of the herb by sinking the stem in 10% NaOH solution for one week. The diffraction pattern of the fibre is found out to be cellulose-I. The effect of the fibre structure and its orientation due to different mounting have been investigated using X-ray diffraction technique. The I max of cellulose-I has been observed along (002) and (10\\overline{1)} for the perpendicular and parallel mounting of the native-fibre, respectively. Full width at half maxima of the diffraction profile turns out to be decreased with fibre orientation. Dimension of crystallite size D hkl estimated in the perpendicular mounting D_{hkl}^{ \\bot } is more as compared to that of the parallel mounting D_{hkl}^{{^{allel } }}. The smallest crystallite sizes observed in both parallel and perpendicular mounting are 18.78 and 30.78 Å respectively. It is expected that the present study may help to analyse the X-ray diffraction of fibre materials in general and natural fibres in particular.

  7. Efecto del extracto alcohólico de Mimosa pudica (mimosa sobre la fertilidad en ratas

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    Jorge Arroyo

    2010-12-01

    Full Text Available Objetivos: Determinar si la administración por vía oral del extracto etanólico de las hojas de Mimosa pudica (mimosa modifica la fertilidad en ratas normales. Diseño: Estudio experimental. Institución: Instituto de Investigaciones Clínicas, Facultad de Medicina, Facultad de Farmacia, Universidad Nacional Mayor de San Marcos y Hospital Nacional Hipólito Unanue, Lima, Perú. Material biológico: Ratas albinas, y hojas de Mimosa pudica. Métodos: Cuarentiocho animales fueron divididos aleatoriamente en grupos de seis animales cada uno. El primero fue control, con solución de suero fisiológico 5 mL/kg, y los siguientes recibieron extracto vía oral 50, 250 y 500 mg/kg, durante 21 días. Cada grupo consideró 6 hembras y 6 machos juntos. Los animales fueron sacrificados para observar la presencia de fetos en el útero. A las hembras se les extrajo muestra de sangre, para conocer el nivel de FSH, estradiol y progesterona, expresándose en µg/dL. El dosaje hormonal se realizó por el método de electroquimioluminiscencia. En el estudio de antiimplantación se usó 2 grupos de 5 ratas hembras grávidas; un grupo recibió agua y el otro recibió el extracto de la planta, en dosis de 600 mg/ kg, durante 10 días. Principales medidas de resultados: Disminución del número de fetos y niveles hormonales. Resultados: Los flavonoides, compuestos fenólicos y taninos estuvieron en mayor cantidad en el extracto etanólico. Las ratas que recibieron 250 mg/kg presentaron mayor número de fetos, seguidas por las de 50 mg/kg; en tanto que con la dosis de 500 mg/kg disminuyó el número de fetos comparativamente con las ratas que no recibieron la planta; hubo incremento de FSH y de progesterona. Conclusiones: En condiciones experimentales se muestra que en dosis de hasta 250 mg/kg aumenta la fertilidad, pero a 500 mg se reduce la fertilidad en ratas normales.

  8. Chemical Constituents of Phacellaria compressa Benth.

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Two new compounds, 1-(3-methoxy-4-hydroxyphenyl)-3-(3, 5-dimethoxy-4-hydroxyphenyl)-propane (1) and 5, 7,3'-trimethyoxyflavan-4'-O-β-D-glucopyranoside (2) were isolated from the ethanol extract of the dried aerial parts of Phacellaria compressa Benth., together with 2,3-bis[(4-hydroxy-3,5-dimethoxyphenyl)-methyl]-1,4-butanediol (3), ethyl 3,4,5-trihydroxybenzoate (4), methyl 3, 4, 5-trihydroxybenzoate (5), β-sitosterol (6), 5, 7, 3', 4'-tetrahydroxyflavan (7), lupeol (8), zhebeiresinol (9), quercetin-3-O-α-L-rhamnopyranoside (10), (+)-catechin (11),betulin (12), β-daucosterol (13), (+)-syringaresinol (14), scopoletin (15), and proximadiol (16). The structures of these compounds were determined by spectral evidence or by comparing them with authentic samples.Compound 9 showed α-amylase inhibitory activity of 57.55% at a concentration of 50 μg/mL.

  9. Two diterpene rhamnosides, mimosasides B and C, from Mimosa hostilis.

    Science.gov (United States)

    Ohsaki, Ayumi; Yokoyama, Ritsuko; Miyatake, Haruka; Fukuyama, Yoshiyasu

    2006-12-01

    Two new diterpene rhamnosides, mimosasides B and C (1, 2) were isolated together with mimosaside A (3), a known diterpene rhamnoside (4), four known flavones (5-8), five known flavanones (9-13), and four known chalcones (14-17) from the leaves and twigs of a Brazilian medicinal plant, Mimosa hostilis.

  10. Occurrence and structure of extrafloral nectariesin Pterodon pubescens Benth. and Pterodon polygalaeflorus Benth.

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    Paiva Élder Antônio Sousa e

    2001-01-01

    Full Text Available Extrafloral nectaries (EFNs are structurally variable and widely spread among the angiosperms. The occurrence of EFNs in leaves of Pterodon polygalaeflorus Benth. and Pterodon pubescens Benth. (Fabaceae: Papilionoideae were detected in adult specimens, at the time of production of new buds and flowers. The goals of the present study are to register the occurrence of the EFNs in P. pubescens and P. polygalaeflorus, and provide comparative data on the anatomical structures. The EFNs occur in the rachis and are located under the insertion of each petiolule. Each nectary consists of a small elevation whose apical portion is deeply invaginated, resulting in a depression (secretory pole, a common characteristic of both species. Unicellular, nonglandular trichomes occur along the rachis, being less numerous in P. polygalaeflorus while in P. pubescens they cover the EFNs. The secretory tissue consists of parenchyma cells with dense cytoplasm compactly arranged. The nectar reaches the surface of the EFNs by rupturing the thin cuticle which covers the secretory pole, since both species lack stomata or any other interruption at the epidermis. The basic difference between the two species, in relation to the EFNs, is the density of the pubescence, which is always greater in P. pubescens. Structural and dimensional modifications may be observed, even between basal and apical nectaries in the same rachis, so it does not constitute a taxonomical tool.

  11. Extraction of hyperoside and quercitrin from mimosa (Albizia julibrissin) foliage.

    Science.gov (United States)

    Ekenseair, Adam K; Duan, Lijan; Carrier, Danielle Julie; Bransby, David I; Clausen, Edgar C

    2006-01-01

    Mimosa, an excellent energy crop candidate because of its high growth yield, also contains, on a dry basis, 0.83% hyperoside and 0.90% quercitrin. Hyperoside has been documented as having anti-inflammatory and diurectic properties, whereas quercitrin may play a role in intestinal repair following chronic mucosal injury. Thus, mimosa might first be extracted for important antioxidant compounds and then used as a feedstock for energy production. This article presents results from studies aimed at determining the effect of three extraction parameters (temperature, solvent composition, and time) on the yield of these important quercetin compounds. Conditions are sought which maximize yield and concentration, whereas complementing subsequent biomass pretreatment, hydrolysis and fermentation.

  12. Mechanoreceptor Cells on the Tertiary Pulvini of Mimosa pudica L.

    Science.gov (United States)

    Visnovitz, Tamás; Világi, Ildikó; Varró, Petra; Kristóf, Zoltán

    2007-11-01

    Special red cells were found on the adaxial surface of tertiary pulvini of Mimosa pudica and experiments performed to determine the origin and function of these cells. Using anatomical (light, scanning electron and transmission electron microscopy) and electrophysiological techniques, we have demonstrated that these red cells are real mechanoreceptor cells. They can generate receptor potential following mechanical stimuli and they are in connection with excitable motor cells (through plasmodesmata). We also provide evidence that these red cells are derived from stomatal subsidiary cells and not guard cells. As histochemical studies show red cells contain tannin, which is important in development of action potentials and movements of plants. These cells could be one of unidentified mechanoreceptors of mimosa.

  13. Memory elements in the electrical network of Mimosa pudica L.

    Science.gov (United States)

    Volkov, Alexander G; Reedus, Jada; Mitchell, Colee M; Tuckett, Clayton; Volkova, Maya I; Markin, Vladislav S; Chua, Leon

    2014-01-01

    The fourth basic circuit element, a memristor, is a resistor with memory that was postulated by Chua in 1971. Here we found that memristors exist in vivo. The electrostimulation of the Mimosa pudica by bipolar sinusoidal or triangle periodic waves induce electrical responses with fingerprints of memristors. Uncouplers carbonylcyanide-3-chlorophenylhydrazone and carbonylcyanide-4-trifluoromethoxy-phenyl hydrazone decrease the amplitude of electrical responses at low and high frequencies of bipolar sinusoidal or triangle periodic electrostimulating waves. Memristive behavior of an electrical network in the Mimosa pudica is linked to the properties of voltage gated ion channels: the channel blocker TEACl reduces the electric response to a conventional resistor. Our results demonstrate that a voltage gated K(+) channel in the excitable tissue of plants has properties of a memristor. The discovery of memristors in plants creates a new direction in the modeling and understanding of electrical phenomena in plants.

  14. Antioxidant Flavonoids from Nepeta floccosa Benth.

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    Liaqat Ali

    2015-06-01

    Full Text Available Antioxidants are the substances which significantly delay or prevent the development of age-related degenerative and chronic diseases caused by oxidative damage. Due to this, there is a considerable interest in the identification of lead molecules, which can inhibit the oxidation process or slow it down considerably. Based on the spectrofluorimetric analysis of superoxide and diphenylpicrylhydrazyl radical scavenging ability, the bio-assay guided isolation of methanolic extract of Nepeta floccosa Benth. was carried out. As a result of this study, one new flavanonol named nepetavanol (1 and four known flavonoids; dihydrokaempferol (2, naringenin (3, caulesnarinside (4, and poncirin (5 have been isolated. The structure of the new flavanonol was assigned on the basis of 1H- and 13C-NMR spectra including two-dimensional NMR techniques such as COSY, HMQC, and HMBC experiments and FABMS, EIMS, HR-MS data. Compound 1 , 2, and 3 showed significant activity whereas compounds 4 and 5 showed moderate activity against antioxidant assays.

  15. Evaluation of wound healing activity of root of Mimosa pudica.

    Science.gov (United States)

    Kokane, Dnyaneshwar D; More, Rahul Y; Kale, Mandar B; Nehete, Minakshi N; Mehendale, Prachi C; Gadgoli, Chhaya H

    2009-07-15

    Mimosa pudica, commonly known as touch-me-not, is used in folklore medicine in arresting bleeding and in skin diseases. There was no scientific evidence justifying the use of Mimosa pudica, therefore the present study was aimed at evaluation of wound healing activity of the plant. In the present study the roots of Mimosa pudica were studied for wound healing activity by incorporating the methanolic and the total aqueous extract in simple ointment base B.P. in concentration of 0.5% (w/w), 1% (w/w) and 2% (w/w). Wound healing activity was studied in three types of model in rats viz. excision, incision and estimation of biochemical parameter. In case of the excision wound model wound contraction and period of epithelization was studied while in incision wound model was evaluated by determining tensile strength and hydroxyproline content in the scab. Treatment of wound with ointment containing 2% (w/w) the methanolic and 2% (w/w) the total aqueous extract exhibited significant (P<0.001) wound healing activity. The methanolic and total aqueous extracts were analyzed for total phenols content equivalent to Gallic acid. The content of total phenols was 11% (w/w) and 17% (w/w) in methanolic and total aqueous extract respectively. The methanolic extract exhibited good wound healing activity probably due to phenols constituents.

  16. [Banana tree pests attacking Heliconia latispatha Benth. (Heliconiaceae)].

    Science.gov (United States)

    Watanabe, Maria A

    2007-01-01

    In mid-May 2005, the caterpillars Antichloris eriphia (Fabr.) (Lepidoptera: Arctiidae) and Calligo illioneus (Cramer) (Lepidoptera: Nymphalidae) which are banana tree pests, were found attacking six-month old stalks of Heliconia latispatha Benth., planted near a banana tree plantation in Jaguariuna, SP, Brazil. The attack by C. illioneus is observed by the first time in Brazil.

  17. Design and Implementation of a Bionic Mimosa Robot with Delicate Leaf Swing Behavior

    Directory of Open Access Journals (Sweden)

    Chung-Liang Chang

    2014-12-01

    Full Text Available This study designed and developed a bionic mimosa robot with delicate leaf swing behaviors. For different swing behaviors, this study developed a variety of situations, in which the bionic mimosa robot would display different postures. The core technologies used were Shape Memory Alloys (SMAs, plastic material, and an intelligent control device. The technology particularly focused on the SMAs memory processing bend mode, directional guidance, and the position of SMAs installed inside the plastic material. Performance analysis and evaluation were conducted using two SMAs for mimosa opening/closing behaviors. Finally, by controlling the mimosa behavior with a micro-controller, the optimal strain swing behavior was realized through fuzzy logic control in order to display the different postures of mimosa under different situations. The proposed method is applicable to micro-bionic robot systems, entertainment robots, biomedical engineering, and architectural aesthetics-related fields in the future.

  18. Nodulation and nitrogen fixation by Mimosa spp. in the Cerrado and Caatinga biomes of Brazil.

    Science.gov (United States)

    dos Reis, Fábio Bueno; Simon, Marcelo F; Gross, Eduardo; Boddey, Robert M; Elliott, Geoffrey N; Neto, Nicolau E; Loureiro, M de Fatima; de Queiroz, Luciano P; Scotti, Maria Rita; Chen, Wen-Ming; Norén, Agneta; Rubio, Maria C; de Faria, Sergio M; Bontemps, Cyril; Goi, Silvia R; Young, J Peter W; Sprent, Janet I; James, Euan K

    2010-06-01

    *An extensive survey of nodulation in the legume genus Mimosa was undertaken in two major biomes in Brazil, the Cerrado and the Caatinga, in both of which there are high degrees of endemicity of the genus. *Nodules were collected from 67 of the 70 Mimosa spp. found. Thirteen of the species were newly reported as nodulating. Nodules were examined by light and electron microscopy, and all except for M. gatesiae had a structure typical of effective Mimosa nodules. The endosymbiotic bacteria in nodules from all of the Mimosa spp. were identified as Burkholderia via immunolabelling with an antibody against Burkholderia phymatum STM815. *Twenty of the 23 Mimosa nodules tested were shown to contain nitrogenase by immunolabelling with an antibody to the nitrogenase Fe- (nifH) protein, and using the delta(15)N ((15)N natural abundance) technique, contributions by biological N(2) fixation of up to 60% of total plant N were calculated for Caatinga Mimosa spp. *It is concluded that nodulation in Mimosa is a generic character, and that the preferred symbionts of Brazilian species are Burkholderia. This is the first study to demonstrate N(2) fixation by beta-rhizobial symbioses in the field.

  19. Mimosa diversiplla var. subglabriseta (MimosoIdeae, Leguminosae, a new record for the flora of Paraguay

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    Javier A Luna

    2012-12-01

    Full Text Available The presence of Mimosa diversipila M. Micheli var. subglabriseta Barneby & Fortunato is now reported for first time in Paraguay. A revision of Barneby's initial description of the variety and photographs of one of the Paraguayan specimens are given in this paper.Mimosa diversipila var. subglabriseta (Mimosoideae, Leguminosae, una nueva cita para la flora de Paraguay. Reportamos por primera vez la presencia de Mimosa diversipila M. Micheli var. subglabriseta Barneby & Fortunato en Paraguay. Una revisión de la descripción inicial de Barneby y fotografías de uno de los ejemplares de Paraguay se incluyen en este documento.

  20. Evaluation of diuretic activity of different extracts of Mimosa pudica Linn.

    Science.gov (United States)

    Baghel, A; Rathore, D S; Gupta, V

    2013-10-15

    In that study, Mimosa pudica linn was tested for diuretic activity using the lipschitz test. The ethanolic and aqoues extract of Mimosa pudica Linn. was studied at two dose level 100 and 200 mg kg(-1) b.wt. Furosemide (20 mg kg(-1) b.wt.) was used as standard drug in a 0.9% saline solution. Urine volumes were measured for all the groups up to 5 h. The ethanolic extract of Mimosa pudica linn was exhibited significant diuretic activity at doses of 100 and 200 mg kg(-1) b.wt. by increasing total urine volume and ion concentration of Na+ k+ and Cl-.

  1. Dormancy break in Pithecellobium dulce (Roxb. Benth. seeds

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    Francisco Elder Carlos Bezerra Pereira

    2015-02-01

    Full Text Available This research aimed to evaluate different methods for breaking dormancy in seeds of Pithecellobium dulce (Roxb. Benth., arboreal species of multiple use in the semiarid region of Northeast Brazil. The treatments were: control – intact seeds (T1; immersion in water until it reaches 80 °C (T2, and 100 °C (T3, concentrated sulfuric acid for five minutes (T4, 10 minutes (T5 , and 15 minutes (T6, mechanical scarification with sandpaper number 80 (T7, mechanical scarification with sandpaper number 80 + immersion in water for six hours (T8, and for 12 hours (T9, soaking in water for 24 hours (T10, and for 48 hours (T11. The characteristics evaluated were such: emergency, mean emergency time, shoot length, root length, number of leaves, leaf area and total dry matter. The best method for breaking dormancy of seeds of Pithecellobium dulce (Roxb. Benth. is mechanical scarification with sandpaper number 80.

  2. Volatile Components of Oiti Fruit (Licania tomentosa Benth.

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    Maria A. C. Kaplan

    2010-10-01

    Full Text Available The fresh fruits from Licania tomentosa Benth. (Chrysobalanaceae , were submitted to hydrodistillation in a modified Clevenger-type apparatus. Analysis of volatile oil were performed by GC and GC-MS. Comparison of results with literature data allowed the chemical characterization of 83% of compounds of mixture. Thirteen compounds were identified, three monoterpenes, five aliphatic esters, two alcohols, two ketones and one aldehyde. The alcohols were the major compounds (35.6%, but the esters showed great diversity.

  3. The genus Isodon (Schrad. ex Benth. Spach in Africa and a new genus Rabdosiella Codd (Lamiaceae

    Directory of Open Access Journals (Sweden)

    L. E. Codd

    1984-12-01

    Full Text Available The typification of the genus Isodon (Schrad. ex Benth. Spach and its occurrence in Africa are discussed; an allied genus Rabdosiella Codd is described and the combinations R. calycina (Benth. Codd and R. ternifolia (D.Don Codd (the latter an Indian species are effected.

  4. The genus Isodon (Schrad. ex Benth. Spach in Africa and a new genus Rabdosiella Codd (Lamiaceae

    Directory of Open Access Journals (Sweden)

    L. E. Codd

    1984-12-01

    Full Text Available The typification of the genus Isodon (Schrad. ex Benth. Spach and its occurrence in Africa are discussed; an allied genus Rabdosiella Codd is described and the combinations R. calycina (Benth. Codd and R. ternifolia (D.Don Codd (the latter an Indian species are effected.

  5. Espectro polínico de amostras de mel de Apis mellifera L., coletadas na Bahia Pollen spectrum of samples of Apis mellifera L., honey collected in Bahia State, Brazil

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    AUGUSTA CAROLINA DE CAMARGO CARMELLO MORETI

    2000-01-01

    Full Text Available O espectro polínico encontrado em amostras de mel provenientes de seis municípios do Estado da Bahia foi analisado com o objetivo de contribuir para o conhecimento da flora visitada por Apis mellifera L. 1758 (Hymenoptera: ApidaeA identificação dos tipos polínicos foi feita por meio de literatura especializada e de informações de campo. Foram encontrados 43 tipos de pólen, sendo consideradas como dominantes as espécies Eucalyptus sp. (Myrtaceae, Mimosa verrucosa Benth. (Mimosaceae, M. scabrella Benth. (Mimosaceae e Bauhinia sp. (Caesalpiniaceae. Os tipos acessórios foram Alternanthera ficoidea R.Br. (Amaranthaceae, tipo Compositae (Asteraceae e Cecropia sp. (Moraceae. Destacou-se a participação de Mimosa sp. (Mimosaceae e de outras espécies silvestres na composição dos méis nos municípios considerados, registrando-se ainda a espécie Eucalyptus sp. (Myrtaceae como uma das principais fontes de alimento das abelhas dessa região.Pollen spectrum found in honey samples from six localities of Bahia State, Brazil, was analyzed with the objective to contribute for the knowledge of the plants used by Apis mellifera L., 1758 (Hymenoptera: Apidae. The identification of the pollen types was made by specialized literature and by field information. Two hundred pollen grains were studied in order to determine the percentage and the occurrence of each type. Forty three pollen types were identified, being considered as the predominant types Eucalyptus sp. (Myrtaceae, Mimosa verrucosa Benth. (Mimosaceae, M. scabrella Benth. (Mimosaceae and Bauhinia sp. (Caesalpiniaceae. The accessory pollen types were Alternanthera ficoidea R.Br. (Amaranthaceae, Compositae type (Asteraceae and Cecropia sp. (Moraceae. It is intense the participation of Mimosa sp. (Mimosaceae and other wild species in the honey composition of the six localities considered. Eucalyptus sp. (Myrtaceae is one of the dominant sources of bee food in some regions of Bahia State.

  6. Hyaluronidase and protease activities from Indian snake venoms: neutralization by Mimosa pudica root extract.

    Science.gov (United States)

    Girish, K S; Mohanakumari, H P; Nagaraju, S; Vishwanath, B S; Kemparaju, K

    2004-06-01

    The aqueous root extract of Mimosa pudica dose dependently inhibited the hyaluronidase and protease activities of Indian snakes (Naja naja, Vipera russelii and Echis carinatus) venom. Copyright 2004 Elsevier B.V.

  7. Inhalational and local anesthetics reduce tactile and thermal responses in mimosa pudica.

    Science.gov (United States)

    Milne, A; Beamish, T

    1999-03-01

    In reaction to a variety of stimuli, the sensitive plant mimosa pudica closes its leaflets and drops its stems. The objective was to investigate the effects of anaesthetics on the reaction of mimosa pudica to a variety of stimuli. The ability of the plants leaflets to close (n=4, Q5min.) was tested after exposure to halothane 4% and 6L x min(-1) O2, or 6L x min(-1) O2. Lidocaine 2% or vehicle were administered through the roots, cut stems, or sprayed on the leaves (n=4). The test consisted of stimulating the leaves, by burning their tips (lidocaine experiments) or touching them with a metal rod (halothane); the closing of the leaflets, or the lack thereof was then observed. After 15 min exposure to halothane, the mimosas had slow and incomplete reactions to tactile stimulation. Following 20 min exposure, the plants had no visible reaction to touch, a stimulus which would normally cause the collapse of the entire leaf. After one minute, mimosa leaves sprayed with lidocaine had no reaction to a lit match being touched to the tips of the leaves, contrary to the control in which the leaves collapsed completely. After exposure to lidocaine 2% through the roots and cut stems, the mimosa's reactivity gradually decreased; after four hours the leaves were insensitive or had slow reaction to tactile stimuli. Inhalational and local anesthetics inhibited motor mechanisms of the mimosa plant. This may be a model to elucidate the mechanisms of action of anesthetics.

  8. Total Flavonoids from Mimosa Pudica Protects Carbon Tetrachloride -Induced Acute Liver Injury in Mice

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    Zhen-qin QIU

    2015-03-01

    Full Text Available Objective: To observe the protective effect of total flavonoids from Mimosa pudica on carbon tetrachloride (CCl4-induced acute liver injury in mice. Methods: CCl4-induced acute liver injury model in mice was established. The activity of ALT and AST, the content of serum albumin (Alb and total antioxidant capacity (T-AOC were determined. The content of malondiadehyde (MDA was measured and the activity of superoxide dismutase (SOD was determined. The histopathological changes of liver were observed.Results: Compared with CCl4 modle group, each dose group of total flavonouida from Mimosa pudica couldreduced the activity of ALT and AST in mice obviously (P<0.01, indicating they had remarkably protective effect on CCl4-induced acute liver injury in mice. high and middle dose groups of total flavonouida from Mimosa pudica couldincrease the content of Alb in mice (P<0.01. Each dose group of total flavonouida from Mimosa pudica could enhance the level of T-AOC (P<0.01. each dose group of total flavonouida from Mimosa pudica could lower the content of liver homogenate MDA but enhance the activity of SOD in a dose-depended manner (P<0.01. Conclusion: Total flavones from Mimosa Pudica have obvious protective effect on CCl4-induced acute liver injury in mice.

  9. Tannins quantification in barks of Mimosa tenuiflora and Acacia mearnsii

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    Leandro Calegari

    2016-03-01

    Full Text Available Due to its chemical complexity, there are several methodologies for vegetable tannins quantification. Thus, this work aims at quantifying both tannin and non-tannin substances present in the barks of Mimosa tenuiflora and Acacia mearnsii by two different methods. From bark particles of both species, analytical solutions were produced by using a steam-jacketed extractor. The solution was analyzed by Stiasny and hide-powder (no chromed methods. For both species, tannin levels were superior when analyzed by hide-powder method, reaching 47.8% and 24.1% for A. mearnsii and M. tenuiflora, respectively. By Stiasny method, the tannins levels considered were 39.0% for A. mearnsii, and 15.5% for M. tenuiflora. Despite the best results presented by A. mearnsii, the bark of M. tenuiflora also showed great potential due to its considerable amount of tannin and the availability of the species at Caatinga biome.

  10. Signal transduction in Mimosa pudica: biologically closed electrical circuits.

    Science.gov (United States)

    Volkov, Alexander G; Foster, Justin C; Markin, Vladislav S

    2010-05-01

    Biologically closed electrical circuits operate over large distances in biological tissues. The activation of such circuits can lead to various physiological and biophysical responses. Here, we analyse the biologically closed electrical circuits of the sensitive plant Mimosa pudica Linn. using electrostimulation of a petiole or pulvinus by the charged capacitor method, and evaluate the equivalent electrical scheme of electrical signal transduction inside the plant. The discharge of a 100 microF capacitor in the pulvinus resulted in the downward fall of the petiole in a few seconds, if the capacitor was charged beforehand by a 1.5 V power supply. Upon disconnection of the capacitor from Ag/AgCl electrodes, the petiole slowly relaxed to the initial position. The electrical properties of the M. pudica were investigated, and an equivalent electrical circuit was proposed that explains the experimental data.

  11. Effect of carbon monoxide on plants. [Mimosa pudica

    Energy Technology Data Exchange (ETDEWEB)

    Zimmerman, P.W.; Crocker, W.; Hitchcock, A.E.

    1933-01-01

    Of 108 species of plants treated with one per cent carbon monoxide, 45 showed epinastic growth of leaves. Several species showed hyponasty which caused upward curling of leaves. Other effects included: retarded stem elongation; abnormally small new leaves; abnormal yellowing of the leaves, beginning with the oldest; abscission of leaves usually associated with yellowing; and hypertrophied tissues on stems and roots. During recovery an abnormally large number of side shoots arose from latent buds of many species. Motion pictures of Mimosa pudica showed a loss of correlation, normal equilibrium position to gravity, and sensitiveness to contact or heat stimuli; however, the leaves moved about more rapidly than those of controls. Since carbon monoxide causes growth rigor and loss of sensitiveness to external stimuli, it is here considered as an anesthetic.

  12. Mimosa pudica: Electrical and mechanical stimulation of plant movements.

    Science.gov (United States)

    Volkov, Alexander G; Foster, Justin C; Ashby, Talitha A; Walker, Ronald K; Johnson, Jon A; Markin, Vladislav S

    2010-02-01

    Thigmonastic movements in the sensitive plant Mimosa pudica L., associated with fast responses to environmental stimuli, appear to be regulated through electrical and chemical signal transductions. The thigmonastic responses of M. pudica can be considered in three stages: stimulus perception, electrical signal transmission and induction of mechanical, hydrodynamical and biochemical responses. We investigated the mechanical movements of the pinnae and petioles in M. pudica induced by the electrical stimulation of a pulvinus, petiole, secondary pulvinus or pinna by a low electrical voltage and charge. The threshold value was 1.3-1.5 V of applied voltage and 2 to 10 microC of charge for the closing of the pinnules. Both voltage and electrical charge are responsible for the electro-stimulated closing of a leaf. The mechanism behind closing the leaf in M. pudica is discussed. The hydroelastic curvature mechanism closely describes the kinetics of M. pudica leaf movements.

  13. Real-time imaging of pulvinus bending in Mimosa pudica.

    Science.gov (United States)

    Song, Kahye; Yeom, Eunseop; Lee, Sang Joon

    2014-09-25

    Mimosa pudica is a plant that rapidly shrinks its body in response to external stimuli. M. pudica does not perform merely simple movements, but exhibits a variety of movements that quickly change depending on the type of stimuli. Previous studies have investigated the motile mechanism of the plants from a biochemical perspective. However, an interdisciplinary study on the structural characteristics of M. pudica should be accompanied by biophysical research to explain the principles underlying such movements. In this study, the structural characteristics and seismonastic reactions of M. pudica were experimentally investigated using advanced bio-imaging techniques. The results show that the key factors for the flexible movements by the pulvinus are the following: bendable xylem bundle, expandable/shrinkable epidermis, tiny wrinkles for surface modification, and a xylem vessel network for efficient water transport. This study provides new insight for better understanding the M. pudica motile mechanism through structural modification.

  14. [Chemical constituents of C-glycosylflavones from Mimosa pudica].

    Science.gov (United States)

    Yuan, Ke; Lü, Jie-Li; Yin, Ming-Wen

    2006-05-01

    To study chemical constituents of Mimosa pudica of Hainan province. The constituents were separated and purified by column chromatography with macroporous adsorption resin Diaion HP-20, Sephadex LH-20, Toyopearl HW-40, MCI Gel CHP-20, RP-18 and normal phase silica gel. Their structures were identified on the basis of physical and spectral data. Four compounds were isolated and identified as: 7, 8, 3', 4'-tetrahydroxyl-6-C-[alpha-L-rhamnopyranosyl-(1 --> 2)]-beta-D-glucopyranosyl flavone (I); 5, 7, 4'-trihydroxyl-8-C-[alpha-L-rhamnopyranosyl-( --> 2)]-beta-D-glucopyranosyl flavone (II); 5, 7, 3', 4'-tetrahydroxyl-6-C-[alpha-L-rhamnopyranosyl-(1 --> 2)]-beta-D-glucopyranosyl flavone (III); catcher (IV). Compound I is a new compound and componuds II - IV were isolated from this plant for the first time.

  15. Biocompatibility of chitosan/Mimosa tenuiflora scaffolds for tissue engineering

    Energy Technology Data Exchange (ETDEWEB)

    Martel-Estrada, Santos Adriana [Instituto de arquitectura diseño y arte, Universidad Autónoma de Ciudad Juárez, Ave. Del Charro #610 norte, Col. Partido Romero, C.P. 32320 Cd. Juárez, Chihuahua (Mexico); Rodríguez-Espinoza, Brenda [Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, Anillo envolvente del PRONAF y Estocolmo, C.P. 32320 Cd. Juárez, Chihuahua (Mexico); Santos-Rodríguez, Elí [ICTP Meso-American Centre for Theoretical Physics (ICTP-MCTP)/Universidad Autónoma de Chiapas, Ciudad Universitaria, Carretera Zapata Km. 4, Real del Bosque (Terán), C.P. 29040 Tuxtla Gutiérrez, Chiapas (Mexico); Jiménez-Vega, Florinda [Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, Anillo envolvente del PRONAF y Estocolmo, C.P. 32320 Cd. Juárez, Chihuahua (Mexico); García-Casillas, Perla E.; Martínez-Pérez, Carlos A. [Instituto de Ingeniería y Tecnología, Universidad Autónoma de Ciudad Juárez, Ave. Del Charro #610 norte, Col. Partido Romero, C.P. 32320 Cd. Juárez, Chihuahua (Mexico); and others

    2015-09-15

    Highlights: • The porosity of the composites allow biological processes for the cell adaptation on the scaffolds. • The composites improve the viability and proliferation of cells. • Composition of the scaffold plays an important role in the biocompatibility. • The results indicate that Mimosa Tenuiflora can induce the differentiation of osteoblast cells. - Abstract: In search of a plant that exhibits osteogenic activity, Mimosa tenuiflora (M. tenuiflora) cortex represents the opportunity to create a biomaterial that, together with the chitosan, is osteoconductive and promote better and rapid regeneration of bone tissue. Thus, the composite of chitosan/M. tenuiflora cortex fabricated will have properties of biocompatibility and allow the osteoblast proliferation. Composites were developed with different concentrations of chitosan/M. tenuiflora cortex (w/w) using thermally induced phase separation technique (TIPS). To analyze the effects of composite on osteoblasts, primary cultures, each sample was collected on days 1, 3 and 7 after seeding. The evaluation of composites consisted of viability and proliferation tests in which we observed the metabolic activity of the cells using MTT reagent and determined the DNA concentration by means of fluorescence. The expression of the marker alkaline phosphatase (ALP) using p-nitrophenyl phosphate was examined, allowing the observation to the activity of proliferation and differentiation of osteoblastic cells. Moreover, an analysis of biomineralization was performed using scanning electron microscopy (SEM), energy dispersive spectroscopy, infrared spectroscopy and X-ray diffraction. The results showed that 80/20 chitosan/M. tenuiflora cortex biocomposite has the best performance with osteoblasts compared to biomaterials 100/0 and 70/30 chitosan/M. tenuiflora composites. Finally, it was determined that the composite of chitosan/M. tenuiflora cortex presents no cytotoxicity and increases the capacity of the osteoblasts

  16. Efeitos potencialmente alelopáticos dos óleos essenciais de Piper hispidinervium C. DC. e Pogostemon heyneanus Benth sobre plantas daninhas Potentially allelopathic effects of the essential oils of Piper hispidinervium C. DC. and Pogostemon heyneanus (Benth on weeds

    Directory of Open Access Journals (Sweden)

    Antônio Pedro da Silva Souza Filho

    2009-01-01

    Full Text Available Este trabalho teve por objetivo caracterizar a atividade potencialmente alelopática de óleos essenciais de pimenta longa (Piper hispidinervium C. DC. e oriza (Pogostemon heyneanus Benth e analisar, comparativamente, seus efeitos alelopáticos. Óleos essenciais obtidos foram preparados em concentrações de 0,25, 0,5 e 1,0%, tendo como eluente o éter metílico, e testados sobre a germinação de sementes, desenvolvimento da radícula e do hipocótilo das plantas daninhas de área de pastagens cultivadas, malícia (Mimosa pudica e mata-pasto (Senna obtusifolia. Os óleos das duas espécies evidenciaram atividade alelopática em intensidades que variaram em função da concentração do óleo, da espécie doadora, da planta receptora e do fator da planta analisado. A germinação das sementes foi o fator mais intensamente inibido pelos óleos. As intensidades das inibições estiveram positivamente associadas à concentração, com inibições máximas verificadas a 1,0%. Malícia foi à espécie receptora mais sensível aos efeitos do óleo. Comparativamente, o óleo essencial da pimenta longa revelou maior potencial para inibir a germinação e o desenvolvimento das duas plantas receptoras, notadamente em relação à germinação de sementes, quando as diferenças foram mais marcantes. Os resultados foram atribuídos à composição química dos óleos, especialmente em relação à presença de monoterpenos, monoterpenos oxigenados e sesquiterpenos.This study aimed to characterize the potential allelopathic activity of essential oils of pimenta longa (Piper hispidinervium C. DC. and oriza (Pogostemon heyneanus Benth, and to examine, comparatively, their allelopathic effects. Essential oils obtained were prepared at concentrations of 0.25%, 0.5% and 1.0%, with ether methanol as eluent, and tested upon the germination of seeds and the development of the radicle and the hypocotyl of the weeds of cultivated grassland areas, malícia (Mimosa

  17. Phytochemical and Bioactivity Evaluation of Scrophularia amplexicaulis Benth.

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    Ardalan Pasdaran

    2016-01-01

    Full Text Available Scrophularia amplexicaulis Benth. is an Iranian endemic species of the genus Scrophularia, which comprises ca. 200 medicinally important herbaceous flowering plants . Phytochemical investigation of the methanol extract of the aerial parts of this species afforded two iridoid glycosides, scropolioside D (1 and scrophuloside B 4 (2, and two phenylalkanoid glycosides, salidroside (3 and verbascoside (4. S tructures of these compounds were determined by comprehensive spectroscopic analyses. Free-radical-scavenging activity, potential antimalarial property, and contact toxicity as well as general toxicity of the extract and fractions were assessed.

  18. Diploflavone, a New Flavonoid from Diplotropis ferruginea Benth. (Fabaceae)

    Energy Technology Data Exchange (ETDEWEB)

    Almeida, Jackson Roberto G.S. [Universidade Federal do Vale do Sao Francisco, Petrolina, PE (Brazil); Barbosa-Filho, Jose Maria; Cabral, Analucia G.S.; Agra, Maria de Fatima; Silva, Marcelo S. da [Universidade Federal da Paraiba, Joao Pessoa, PB (Brazil). Lab. de Tecnologia Farmaceutica]. E-mail: jbarbosa@ltf.ufpb.br; Da Cunha, Emidio V. Leitao [Universidade Estadual da Paraiba, Campina Grande, PB (Brazil). Dept. de Farmacia; Nascimento, Silene C. do [Universidade Federal de Pernambuco, Recife, PE (Brazil). Dept. de Antibioticos; Braz-Filho, Raimundo [Universidade Estadual do Norte Fluminense (UENF), Campos dos Goytacases, RJ (Brazil). Setor de Quimica de Produtos Naturais. Lab. de Ciencias Quimicas

    2005-11-15

    The chemical examination of Diplotropis ferruginea Benth. resulted in the isolation of a new 3-methoxyflavone, 3-methoxy-6-O-prenyl-6,6-dimethylchromene-(7,8,2,3)-flavone, to which was given the trivial name diploflavone (1); as well as the known 3,6-dimethoxy-6,6- dimethylchromene-(7,8,2,3)-flavone (2). The structure of the new compound was established by spectral analyses. Cytotoxic activity of the isolated compounds was tested against the cells NClH292 (lung carcinoma), HEp-2 (larynx carcinoma) and KB (oral epidermoid carcinoma). The cells HEp-2 were the most affected by the substances tested. (author)

  19. Chemical Constituents of the Roots of Vernonia cumingiana Benth.

    Institute of Scientific and Technical Information of China (English)

    Qing-Hua LIU; Jing-Quan YUAN; Mao-Rong SUO; Jun-Shan YANG

    2005-01-01

    To search for new and bioactive constituents from traditional Chinese medicines, a new steroidal saponin, named vernonioside G (1), was isolated from the roots of Vernonia cumingiana Benth. (Compositae).The structure of vernonioside G was elucidated using spectral methods, particularly two-dimensional nuclear magnetic resonance analysis. Together with the new compound, eight known compounds were also isolated and identified from the roots of V. cumingiana, among which, VE-1 (2) and 24-methylenelanost-9(11)-en-3β-ol acetate (3) were assigned NMR data for the first time and compound 3 was obtained as a natural product from a plant for the first time.

  20. EVALUATION OF LEATHER QUALITY AND ECOTOXICITY IN SIMULATED TANNERY WASTEWATERS USING MIMOSA TANNIN

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    ÇELİK Cem

    2016-05-01

    Full Text Available The leather tanning industry is characterized by the production of different kinds of effluents, generated in each step of leather processing. These effluents have various chemical compounds which may cause toxicity and endocrine disruption and are thus known as endocrine disrupting chemicals (EDC. Tanning stabilizes the protein structure of the hide and imparts heat stability, enhanced tensile properties, and resistance to microbial degradation. Currently most high quality leather is "chrome-tanned," produced by treatment of the hide with salts of the mineral chromium. In this study, the wastewater characteristics and ecotoxicity before and after tanning and retanning processes using mimosa tannin are assessed. Vegetable leather production procedure was followed using one dose mimosa tannin. Leather quality was evaluated according to standard methods. Wastewater characteristics showed that mimosa contributed high organic content to the wastewater. Although vegetable tannin was used the effluent toxicity was observed in tanning and retanning effluents. The preliminary results also showed that leather quality tests failed or at minimum level to comply with the standard values indicating that there is still a need to optimize the procedure including mimosa dose. This study was designed to produce eco-friendly leather using mimosa in tanning and retanning processes. Leather quality and the ecotoxicity of each process during leather production was assessed according to standard methods.

  1. Chitosan/Mimosa tenuiflora films as potential cellular patch for skin regeneration.

    Science.gov (United States)

    Valencia-Gómez, Laura Elizabeth; Martel-Estrada, Santos Adriana; Vargas-Requena, Claudia; Rivera-Armenta, José Luis; Alba-Baena, Noe; Rodríguez-González, Claudia; Olivas-Armendáriz, Imelda

    2016-12-01

    Bio-composites films were prepared by casting and drying of aqueous solutions containing different weight ratios of chitosan and bark of Mimosa tenuiflora. The physico-chemical and functional properties of the films were characterized by scanning electron microscopy, dynamical mechanical analysis, wettability, cytotoxicity and in vitro antibacterial activities. The morphology studies confirmed that the presence of Mimosa tenuiflora change the surface of films. Moreover, the incorporation of Mimosa tenuiflora improved the thermal stability of the films, as it was indicated by the changes in the glass temperatures obtained. Water-uptake ability changed in relation to polymeric composition of film. This property increased by the addition of Mimosa tenuiflora to the film. Improved antibacterial properties were measured against Escherichia Coli and Micrococcus lysodeikticus or luteus. Finally, cytotoxicity was studied by MTT assay and the films were non-toxic. These preliminary results provide a cheap way to prepare chitosan/Mimosa tenuiflora films for wound healing and skin regeneration. Copyright © 2016 Elsevier B.V. All rights reserved.

  2. EVALUATION OF ANTIBACTERIAL ACTIVITY OF ETHANOLIC EXTRACTS OF MIMOSA PUDICA LEAVES

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    M.SUNIL

    2016-07-01

    Full Text Available Objective: This study was carried out with an objective to investigate the antibacterial activity of ethanolic extracts of mimosa pudica. Methods: In the present study, the anti microbial activity of ethanolic extracts of Mimosa pudica was evaluated against medically important bacterial strains, two Gram-positive—Staphylococcus aureus and Streptococcus pyogenes and two Gram-negative—Escherichia coli and Pseudomonas aeruginosa. The antimicrobial activity was determined with the extract using agar disc diffusion method. The antibacterial activities of extracts (5, 25, 50, 100, 250 μgm/ml of Mimosa pudica were tested against human pathogenic bacteria. Zone of inhibition of extracts were compared with the standard drug ampicillin for antibacterial activity. Results: The results showed that the Mimosa pudica has the antibacterial activity. Conclusion: The antimicrobial activity of Mimosa pudica was due to the presence of various secondary metabolites. Hence, these plants can be used to discover bioactive natural products that may serve as leads in the development of new pharmaceuticals research activities.

  3. Coexistence of Burkholderia, Cupriavidus, and Rhizobium sp. nodule bacteria on two Mimosa spp. in Costa Rica.

    Science.gov (United States)

    Barrett, Craig F; Parker, Matthew A

    2006-02-01

    rRNA gene sequencing and PCR assays indicated that 215 isolates of root nodule bacteria from two Mimosa species at three sites in Costa Rica belonged to the genera Burkholderia, Cupriavidus, and Rhizobium. This is the first report of Cupriavidus sp. nodule symbionts for Mimosa populations within their native geographic range in the neotropics. Burkholderia spp. predominated among samples from Mimosa pigra (86% of isolates), while there was a more even distribution of Cupriavidus, Burkholderia, and Rhizobium spp. on Mimosa pudica (38, 37, and 25% of isolates, respectively). All Cupriavidus and Burkholderia genotypes tested formed root nodules and fixed nitrogen on both M. pigra and M. pudica, and sequencing of rRNA genes in strains reisolated from nodules verified identity with inoculant strains. Inoculation tests further indicated that both Cupriavidus and Burkholderia spp. resulted in significantly higher plant growth and nodule nitrogenase activity (as measured by acetylene reduction assays) relative to plant performance with strains of Rhizobium. Given the prevalence of Burkholderia and Cupriavidus spp. on these Mimosa legumes and the widespread distribution of these plants both within and outside the neotropics, it is likely that both beta-proteobacterial genera are more ubiquitous as root nodule symbionts than previously believed.

  4. QUANTITATIVE DETERMINATION OF THE AMOUNT OF FLAVONOIDS IN THE HERB OF SALVIA FARINACEA BENTH.

    Directory of Open Access Journals (Sweden)

    O. I. Popova

    2016-01-01

    Full Text Available Chromatographic researches conducted have allowed determination of the flavonoids presence in raw materials of Salvia farinacea Benth. They were rutin, quercetin, hyperoside, and 5 phenol carbonic acids: caffeic, p-coumaric, ferulic, gallic, and cinnamic acids. The quantitative content of the sum of flavonoids in Salvia farinacea Benth. was conducted by the method of differential spectrophotometry, and it amounted to 0.55-0.60% in terms of rutin. The research conducted made it possible to expand the scientific data of phenolic compounds of Salvia farinacea Benth. and to offer the possibility of recycling, monitoring procurement and targeted use of Salvia farinacea Benth. herb in pharmacy and medicine.

  5. The Jurema tree (Mimosa hostilis) as an energy source for the Brazilian arid northeast region; A Jurema preta (Mimosa hostilis, Benth) como fonte energetica do semi-arido do nordeste-carvao

    Energy Technology Data Exchange (ETDEWEB)

    Faria, Washington Luiz Fonseca

    1984-12-31

    This study was carried out to evaluate the charcoal production using `Jurema` wood and also to determine its potential as an alternative energy source for Paraiba state, northeast Brazil. Charcoal was produced in a laboratory electrical retort at temperatures of 400, 550 and 700 deg C. The following properties were analyzed: yield (condensed liquids and non-condensable gases), superior calorific value, specific gravity, volatile materials, fixed carbon and ash content. (author). 18 figs., 17 tabs., 47 refs

  6. Assessment of mutagenic, antimutagenic and genotoxicity effects of Mimosa tenuiflora

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    Viviane A. Silva

    2013-04-01

    Full Text Available Genotoxic effects of Mimosa tenuiflora (Willd. Poir, Fabaceae, were investigated by using both micronucleus test and bacterial reverse mutation assay in Salmonella typhimurium TA97, TA98, TA100, TA102 respectively. In respect of Ames test results show that the extract does not induce mutations in any strains of Salmonella typhimurium tested since the mutagenicity index is less than 2. In the antimutagenic effect was observed that the extract at the concentrations tested significantly decreased the mutagenicity index of all strains tested which characterized the extract as antimutagenic in these conditions. In the micronucleus test in vivo, we observed that the concentrations used did not induce an increase in the frequency of micronucleus in normochromatic erythrocytes of mice. Therefore, we concluded that the extract of M. tenuiflora is not mutagenic in the absence of exogenous metabolizing system and does not induce an increase in the frequency of the micronucleus characterized as an agent not mutagenic in these conditions. Further studies of toxicity need to be made to the use of this plant in the treatment of diseases to be stimulated.

  7. An invasive Mimosa in India does not adopt the symbionts of its native relatives.

    Science.gov (United States)

    Gehlot, Hukam Singh; Tak, Nisha; Kaushik, Muskan; Mitra, Shubhajit; Chen, Wen-Ming; Poweleit, Nicole; Panwar, Dheeren; Poonar, Neetu; Parihar, Rashmita; Tak, Alkesh; Sankhla, Indu Singh; Ojha, Archana; Rao, Satyawada Rama; Simon, Marcelo F; Reis Junior, Fabio Bueno Dos; Perigolo, Natalia; Tripathi, Anil K; Sprent, Janet I; Young, J Peter W; James, Euan K; Gyaneshwar, Prasad

    2013-07-01

    The large monophyletic genus Mimosa comprises approx. 500 species, most of which are native to the New World, with Central Brazil being the main centre of radiation. All Brazilian Mimosa spp. so far examined are nodulated by rhizobia in the betaproteobacterial genus Burkholderia. Approximately 10 Mya, transoceanic dispersal resulted in the Indian subcontinent hosting up to six endemic Mimosa spp. The nodulation ability and rhizobial symbionts of two of these, M. hamata and M. himalayana, both from north-west India, are here examined, and compared with those of M. pudica, an invasive species. Nodules were collected from several locations, and examined by light and electron microscopy. Rhizobia isolated from them were characterized in terms of their abilities to nodulate the three Mimosa hosts. The molecular phylogenetic relationships of the rhizobia were determined by analysis of 16S rRNA, nifH and nodA gene sequences. Both native Indian Mimosa spp. nodulated effectively in their respective rhizosphere soils. Based on 16S rRNA, nifH and nodA sequences, their symbionts were identified as belonging to the alphaproteobacterial genus Ensifer, and were closest to the 'Old World' Ensifer saheli, E. kostiensis and E. arboris. In contrast, the invasive M. pudica was predominantly nodulated by Betaproteobacteria in the genera Cupriavidus and Burkholderia. All rhizobial strains tested effectively nodulated their original hosts, but the symbionts of the native species could not nodulate M. pudica. The native Mimosa spp. in India are not nodulated by the Burkholderia symbionts of their South American relatives, but by a unique group of alpha-rhizobial microsymbionts that are closely related to the 'local' Old World Ensifer symbionts of other mimosoid legumes in north-west India. They appear not to share symbionts with the invasive M. pudica, symbionts of which are mostly beta-rhizobial.

  8. Novel Cupriavidus Strains Isolated from Root Nodules of Native Uruguayan Mimosa Species.

    Science.gov (United States)

    Platero, Raúl; James, Euan K; Rios, Cecilia; Iriarte, Andrés; Sandes, Laura; Zabaleta, María; Battistoni, Federico; Fabiano, Elena

    2016-06-01

    The large legume genus Mimosa is known to be associated with both alphaproteobacterial and betaproteobacterial symbionts, depending on environment and plant taxonomy, e.g., Brazilian species are preferentially nodulated by Burkholderia, whereas those in Mexico are associated with alphaproteobacterial symbionts. Little is known, however, about the symbiotic preferences of Mimosa spp. at the southern subtropical limits of the genus. In the present study, rhizobia were isolated from field-collected nodules from Mimosa species that are native to a region in southern Uruguay. Phylogenetic analyses of sequences of the 16S rRNA, recA, and gyrB core genome and the nifH and nodA symbiosis-essential loci confirmed that all the isolates belonged to the genus Cupriavidus However, none were in the well-described symbiotic species C. taiwanensis, but instead they were closely related to other species, such as C. necator, and to species not previously known to be symbiotic (or diazotrophic), such as C. basilensis and C. pinatubonensis Selection of these novel Cupriavidus symbionts by Uruguayan Mimosa spp. is most likely due to their geographical separation from their Brazilian cousins and to the characteristics of the soils in which they were found. With the aim of exploring the diversity of rhizobia associated with native Mimosa species, symbionts were isolated from root nodules on five Mimosa species that are native to a region in southern Uruguay, Sierra del Abra de Zabaleta. In contrast to data obtained in the major centers of diversification of the genus Mimosa, Brazil and Mexico, where it is mainly associated with Burkholderia and Rhizobium/Ensifer, respectively, the present study has shown that all the isolated symbiotic bacteria belonged to the genus Cupriavidus Interestingly, none of nodules contained bacteria belonging to the well-described symbiotic species C. taiwanensis, but instead they were related to other Cupriavidus species such as C. necator and C

  9. Novel Cupriavidus Strains Isolated from Root Nodules of Native Uruguayan Mimosa Species

    Science.gov (United States)

    James, Euan K.; Rios, Cecilia; Iriarte, Andrés; Sandes, Laura; Zabaleta, María; Battistoni, Federico; Fabiano, Elena

    2016-01-01

    ABSTRACT The large legume genus Mimosa is known to be associated with both alphaproteobacterial and betaproteobacterial symbionts, depending on environment and plant taxonomy, e.g., Brazilian species are preferentially nodulated by Burkholderia, whereas those in Mexico are associated with alphaproteobacterial symbionts. Little is known, however, about the symbiotic preferences of Mimosa spp. at the southern subtropical limits of the genus. In the present study, rhizobia were isolated from field-collected nodules from Mimosa species that are native to a region in southern Uruguay. Phylogenetic analyses of sequences of the 16S rRNA, recA, and gyrB core genome and the nifH and nodA symbiosis-essential loci confirmed that all the isolates belonged to the genus Cupriavidus. However, none were in the well-described symbiotic species C. taiwanensis, but instead they were closely related to other species, such as C. necator, and to species not previously known to be symbiotic (or diazotrophic), such as C. basilensis and C. pinatubonensis. Selection of these novel Cupriavidus symbionts by Uruguayan Mimosa spp. is most likely due to their geographical separation from their Brazilian cousins and to the characteristics of the soils in which they were found. IMPORTANCE With the aim of exploring the diversity of rhizobia associated with native Mimosa species, symbionts were isolated from root nodules on five Mimosa species that are native to a region in southern Uruguay, Sierra del Abra de Zabaleta. In contrast to data obtained in the major centers of diversification of the genus Mimosa, Brazil and Mexico, where it is mainly associated with Burkholderia and Rhizobium/Ensifer, respectively, the present study has shown that all the isolated symbiotic bacteria belonged to the genus Cupriavidus. Interestingly, none of nodules contained bacteria belonging to the well-described symbiotic species C. taiwanensis, but instead they were related to other Cupriavidus species such as C

  10. Propagação vegetativa de Hyptis leucocephala Mart. ex Benth. e Hyptis platanifolia Mart. ex Benth. (Lamiaceae Vegetative propagation of Hyptis leucocephala Mart. ex Benth. and Hyptis platanifolia Mart. ex Benth

    Directory of Open Access Journals (Sweden)

    L.M Oliveira

    2011-01-01

    Full Text Available O gênero Hyptis (Lamiaceae apresenta metabolismo especial de notável variabilidade, possuindo predominância de óleos essenciais, que têm muito valor junto a diversas comunidades que utilizam essas plantas pelas propriedades terapêuticas. Diversos estudos com esse gênero têm demonstrado que os óleos essenciais apresentam propriedades antimicrobiana, antifúngica, citotóxica, antiinflamatória, anti-HIV e inseticida, entretanto, poucos são os estudos voltados para a exploração sustentável dessas espécies. O objetivo desse trabalho foi avaliar o efeito de diferentes tipos de estaca e diferentes concentrações de ácido indolbutírico (AIB sobre a taxa de sobrevivência, crescimento e enraizamento de estacas de Hyptis leucocephala Mart. ex Benth. e Hyptis platanifolia Mart. ex Benth., espécies endêmicas do semi-árido nordestino cujo valor medicinal tem sido amplamente comprovado. O experimento teve duração de quatro meses. Foram utilizadas estacas apicais, médio-apicais, médio-basais e basais tratadas com soluções de 0, 1000, 2000 e 4000 mg L-1 de ácido indolbutírico (AIB, durante 5 minutos. A utilização de estacas apicais, médio/apicais e médio/basais induzidas com 2000 e 4000 mg L-1 de AIB possibilitaram melhores resultados para propagação vegetativa de Hyptis leucocephala. Para Hyptis platanifolia os melhores resultados foram obtidos com a utilização de estacas médio/basais e basais tratadas com AIB na concentração de 2000 e 4000 mg L-1.The genus Hyptis (Lamiaceae has a special metabolism with remarkable variability and predominance of essential oils of great value to the various communities that use these plants due to their therapeutic properties. A number of studies on this genus have demonstrated that its essential oils present antimicrobial, antifungal, cytotoxic, anti-inflammatory, anti-HIV and insecticidal activities. However, few studies have focused on the sustainable exploitation of these species

  11. Biometria de frutos e sementes e germinação de Plathymenia reticulata benth. e Plathymenia foliolosa benth. (Fabaceae - mimosoideae Biometry of fruits and seeds and germination of Plathymenia reticulata benth. and Plathymenia foliolosa benth. (Fabaceae - mimosoideae

    Directory of Open Access Journals (Sweden)

    Renata de Melo Ferreira Lopes

    2010-10-01

    Full Text Available Este estudo objetivou comparar características biométricas de frutos e sementes e o efeito de diferentes escarificações, temperaturas e luz na germinação de Plathymenia reticulata Benth. e Plathymenia foliolosa Benth. Foram registrados comprimento, largura, espessura, massa da matéria seca e fresca de frutos (n = 100 e sementes (n = 100 de cada espécie. Os diferentes tratamentos foram escarificações mecânica e química e temperaturas (fotoperíodo/nictoperíodo de 20, 30 e 35/15 °C (12/12 e 0/24 e 25 e 35 °C (12/12. Os frutos de P. foliolosa mostraram-se mais largos, espessos e pesados e as sementes, mais compridas e espessas do que as de P. reticulata. As sementes de ambas as espécies não apresentaram fotoblastismo. A escarificação ácida não aumentou significativamente a germinabilidade das sementes em relação ao grupo-controle, enquanto a escarificação mecânica incrementou significativamente a germinabilidade apenas de P. foliolosa. As germinabilidades a 25 °C das sementes de P. reticulata intactas, escarificadas com ácido e lixa foram, respectivamente, de 55%, 60% e 89%. Para as sementes de P. foliolosa esses valores foram 48%, 37,5% e 83%, respectivamente.Esses resultados apontam limitações na germinação de P. foliolosa impostas pelo tegumento, entretanto o efeito deste restringindo a germinação das sementes intactas decresceu com a elevação da temperatura.This study aimed to compare the biometry of fruits and seeds, as well as the effect of different scarification, temperature and light conditions on the germination of Plathymenia reticulata Benth. and Plathymenia foliolosa Benth. We measured the length, width, thickness, and fresh mass of fruits (n= 100 and seeds (n= 100 of each specie. The different treatments were mechanic and acid scarification, temperatures (photoperiod/nictoperiod of 20, 30 and 35/15 °C (12/12 and 0/24 and 25 and 35 °C (12/12. P. foliolosa fruits were wider, thickener and heavier

  12. Mimosa pudica may possess antidepressant actions in the rat.

    Science.gov (United States)

    Molina, M; Contreras, C M; Tellez-Alcantara, P

    1999-11-01

    In Mexico, aqueous extracts from dried leaves of Mimosa puolica are employed to alleviate depression. In this study, the behavioral actions of aqueous extracts of M. pudica at various concentrations were tested. Rats having received saline (0.9%; 0.30 ml; I.P.), clomipramine, desipramine or several dosages of aqueous extracts from M. pudica (ml = 2.0 mg/kg; m2 = 4.0 mg/kg; m3 = 6.0 mg/kg; m4 = 8.0 mg/kg) during a 30-day period were submitted to the forced swimming test and to the test for differential reinforcement of low rates of response at 72 sec (DRL-72s). Any possible anxiolytic action resulting from several doses (ml = 2.0 mg/kg; m2 = 4.0 mg/kg; m3 = 6.0 mg/kg; m4 = 8.0 mg/kg) of extracts of M. pudica were compared with those caused by diazepam (1.3 mg/kg, I.P.) in the elevated plus-maze test. Results showed that clomipramine (1.25 mg/kg, I.P.), desipramine (2.14 mg/kg, I.P.) and M. pudica (6.0 mg/kg and 8.0 mg/kg, I.P.) reduced immobility in the forced swimming test and increased the rate of reinforcers received in the DRL-72s test; these data suggest that M. pudica produces antidepressant effects in the rat. Diazepam increased the open-arms exploration time in the elevated plus-maze test, but M. pudica did not show any comparable action at any tested dose. M. pudica therefore produced an antide-pressant-like profile similar to two tricyclic antidepressants.

  13. Lesões traumáticas de pele causadas pelos espinhos de Mimosa pudica e Mimosa debilis em equídeos

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    Alessandra S. Belo Reis

    2011-09-01

    Full Text Available Foram realizadas pesquisas sobre a natureza e causa de lesões de pele em equídeos em uma propriedade no município de Castanhal, região Nordeste do Estado do Pará. Foram realizadas visitas técnicas, estudos epidemiológicos, coletas de sangue, biópsias de pele afetada e a inspeção da pastagem. O estudo incluiu 25 equídeos, dos quais 14 machos e 11 fêmeas, de seis meses e oito anos de idade. Os animais apresentaram lesões ulcerativas, de bordos irregulares, na cabeça (narinas, focinho, lábios superiores e inferiores e chanfro, na cavidade oral (vestíbulo bucal e gengiva e nos membros (boletos, metacarpos e metatarsos e articulação escápulo-umeral. No exame histopatológico foram observados focos de erosões cutâneas, caracterizados por perda e necrose da epiderme, com espongiose, degeneração vesicular da epiderme remanescente e leve infiltrado inflamatório na derme subjacente, constituído predominantemente por macrófagos e, em menor grau, eosinófilos. Na inspeção da pastagem, constituída de Brachiaria humidicola, foi constatada grande invasão de duas plantas providas de espinhos, Mimosa pudica e Mimosa debilis, ambas da família Leg. Mimosoideae. Concluiu-se, que as lesões de pele foram causadas pela ação traumática dos espinhos de Mimosa pudica e Mimosa debilis.

  14. Total Flavonoids from Mimosa Pudica Protects Carbon Tetrachloride-Induced Acute Liver Injur y in Mice

    Institute of Scientific and Technical Information of China (English)

    QIU Zhen-qin; CAI Lei; CHEN Da-shuai

    2015-01-01

    Objective:To observe the protective effect of total lfavonoids from Mimosa pudica on carbon tetrachloride (CCl4)-induced acute liver injury in mice. Methods:CCl4-induced acute liver injury model in mice was established. The activity of ALT and AST, the content of serum albumin (Alb) and total antioxidant capacity (T-AOC) were determined. The content of malondiadehyde (MDA) was measured and the activity of superoxide dismutase (SOD) was determined. The histopathological changes of liver were observed. Results:Compared with CCl4 model group, each dose group of total lfavonouida from Mimosa pudica could reduced the activity of ALT and AST in mice obviously (P<0.01), indicating they had remarkably protective effect on CCl4-induced acute liver injury in mice. High and middle dose groups of total lfavonouida from Mimosa pudica could increase the content of Alb in mice (P<0.01). Each dose group of total lfavonouida from Mimosa pudica could enhance the level of T-AOC (P<0.01), and lower the content of liver homogenate MDA, but enhance the activity of SOD in a dose-depended manner (P<0.01).

  15. Inhibition of fungal colonization on the rhizoplane of the CS2 - producing plant, Mimosa pudica L.

    Science.gov (United States)

    Z. Feng; P.G. Hartel; R.W. Roncadori; Shi-Jean S. Sung

    1998-01-01

    Carbon disulfide (CS2) is a colorless, volatile, foul-smelling, fungicidal liquid that is produced by some plants. The authors determined the ability of a model CS2-producing plant, Mimosa pudica, to affect the rhizoplane colonization of six species of soil fungi. Tomato (Lycopersicon esculentum...

  16. Mimosa tenuiflora as a Cause of Malformations in Ruminants in the Northeastern Brazilian Semiarid Rangelands

    Science.gov (United States)

    Craniofacial anomalies, eye malformations, and permanent flexures of the forelimbs are common malformations seen in ruminants grazing semiarid rangelands of Northeastern Brazil. To investigate the cause of these malformations, we fed 2 suspected plants, Mimosa tenuiflora or Prosopis juliflora, to gr...

  17. Mimosa-inspired design of a flexible pressure sensor with touch sensitivity.

    Science.gov (United States)

    Su, Bin; Gong, Shu; Ma, Zheng; Yap, Lim Wei; Cheng, Wenlong

    2015-04-24

    A bio-inspired flexible pressure sensor is generated with high sensitivity (50.17 kPa(-1)), quick responding time (sensor substrates results from the direct molding of natural mimosa leaves, presenting a simple, environment-friendly and easy scale-up fabrication process for these flexible pressure sensors.

  18. Cupriavidus taiwanensis bacteroids in Mimosa pudica Indeterminate nodules are not terminally differentiated.

    Science.gov (United States)

    Marchetti, Marta; Catrice, Olivier; Batut, Jacques; Masson-Boivin, Catherine

    2011-03-01

    The beta-rhizobium Cupriavidus taiwanensis forms indeterminate nodules on Mimosa pudica. C. taiwanensis bacteroids resemble free-living bacteria in terms of genomic DNA content, cell size, membrane permeability, and viability, in contrast to bacteroids in indeterminate nodules of the galegoid clade. Bacteroid differentiation is thus unrelated to nodule ontogeny.

  19. Burkholderia and Cupriavidus spp. are the preferred symbionts of Mimosa spp. in southern China.

    Science.gov (United States)

    Liu, XiaoYun; Wei, Shuang; Wang, Fang; James, Euan K; Guo, XiaoYe; Zagar, Catherine; Xia, Liu Gui; Dong, Xin; Wang, Yi Peng

    2012-05-01

    Rhizobia were isolated from invasive Mimosa spp. (M. diplotricha and M. pudica) in Dehong district of the province of Yunnan in subtropical southern China. Almost all of the 98 isolates were β-rhizobia in the genera Burkholderia and Cupriavidus. These strains were analysed for their distribution characteristics together with strains from a previous study from Sishuangbanna. The proportion of nodules containing each β-rhizobial genus varied between Mimosa species, with Cupriavidus being predominant in M. diplotricha nodules (63.3% compared to 36.7% occupation with Burkholderia), but with M. pudica showing a slight preference for Burkholderia over Cupriavidus, with them occupying 56.5% and 43.5% of nodules, respectively. The symbiosis-essential genes nodA and nifH were present in all the Burkholderia and Cupriavidus strains tested, and their phylogenies indicated that these Mimosa symbionts share symbiotic genes with native South American rhizobia. The evolutionary discrepancies among 16S rRNA genes, nodA and nifH of Mimosa spp. symbionts, suggests that the nod and nif genes of β-rhizobia evolved independently. © 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

  20. Beta-rhizobia from Mimosa pigra, a newly discovered invasive plant in Taiwan.

    Science.gov (United States)

    Chen, Wen-Ming; James, Euan K; Chou, Jui-Hsing; Sheu, Shih-Yi; Yang, Sheng-Zehn; Sprent, Janet I

    2005-12-01

    A total of 191 rhizobial isolates from the root nodules of three geographically separate populations of the invasive plant Mimosa pigra in Taiwan were examined using amplified rDNA restriction analysis, 16S rDNA sequences, protein profiles and ELISA. Of these, 96% were identified as Burkholderia and 4% as Cupriavidus taiwanensis. The symbiosis-essential genes nodA and nifH were present in two strains of Burkholderia (PAS44 and PTK47), and in one of C. taiwanensis (PAS15). All three could nodulate M. pigra. Light and electron microscopy studies with a green fluorescent protein transconjugant variant of strain PAS44 showed the presence of fluorescent bacteroids in M. pigra nodules. These bacteroids expressed the nifH protein, hence this is the first confirmation that Burkholderia is a genuine symbiont of legume nodules. The predominance of Burkholderia in Taiwanese M. pigra suggests that this species may have brought its symbionts from its native South America, rather than entering into association with the Taiwanese Mimosa symbiont C. taiwanensis which so successfully nodulates Mimosa pudica and Mimosa diplotricha.

  1. Nodulation of Mimosa spp. by the beta-proteobacterium Ralstonia taiwanensis.

    Science.gov (United States)

    Chen, Wen-Ming; James, Euan K; Prescott, Alan R; Kierans, Martin; Sprent, Janet I

    2003-12-01

    Several beta-proteobacteria have been isolated from legume root nodules and some of these are thought to be capable of nodulating and fixing N2. However, in no case has there been detailed studies confirming that they are the active symbionts. Here, Ralstonia taiwanensis LMG19424, which was originally isolated from Mimosa pudica nodules, was transformed to carry the green fluorescent protein (gfp) reporter gene before being used to inoculate axenically-grown seedlings of M. pudica and M. diplotricha. Plants were harvested at various intervals for 56 days after inoculation, then examined for evidence of infection and nodule formation. Nodulation of both Mimosa spp. was abundant, and acetylene reduction assays confirmed that nodules had nitrogenase activity. Confocal laser scanning microscopy (CLSM) showed that fresh M. pudica nodules with nitrogenase activity had infected cells containing bacteroids expressing gfp. In parallel, fixed and embedded nodules from both Mimosa spp. were sectioned for light and electron microscopy, followed by immunogold labeling with antibodies raised against gfp and nitrogenase Fe (nifH) protein. Significant immunolabeling with these antibodies confirmed that R. taiwanensis LMG19424 is an effective N2-fixing symbiont of Mimosa spp. Both species were infected via root hairs and, in all respects, the nodule ontogeny and development was similar to that described for other mimosoid legumes. The nodules were indeterminate with a persistent meristem, an invasion zone containing host cells being invaded via prominent infection threads, and an N2-fixing zone with infected cells containing membrane-bound symbiosomes.

  2. Cupriavidus taiwanensis Bacteroids in Mimosa pudica Indeterminate Nodules Are Not Terminally Differentiated ▿

    Science.gov (United States)

    Marchetti, Marta; Catrice, Olivier; Batut, Jacques; Masson-Boivin, Catherine

    2011-01-01

    The beta-rhizobium Cupriavidus taiwanensis forms indeterminate nodules on Mimosa pudica. C. taiwanensis bacteroids resemble free-living bacteria in terms of genomic DNA content, cell size, membrane permeability, and viability, in contrast to bacteroids in indeterminate nodules of the galegoid clade. Bacteroid differentiation is thus unrelated to nodule ontogeny. PMID:21257807

  3. Inhibition of Asthma in OVA Sensitized Mice Model by a Traditional Uygur Herb Nepeta bracteata Benth.

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    Jing Wang

    2016-01-01

    Full Text Available Asthma is a chronic lung inflammation which affects many people. As current therapies for asthma mainly rely on administration of glucocorticoids and have many side effects, new therapy is needed. In this study, we investigated Nepeta bracteata Benth., a traditional Uygur Herb, for its therapeutics effect in OVA induced asthmatic mice model. Treatment of OVA sensitized asthma mice with extract from Nepeta bracteata Benth. demonstrated improved lung pathology, as well as reduced infiltration of eosinophil and neutrophil. Nepeta bracteata Benth. extract also contributed to the rebalance of Th17/Treg cell via decreasing the Th17 cell and increasing the Treg, which was corresponding with the inhibited Th17 cytokine response and increased IL-10 level. Moreover, the reduced TGF-β level and Smad2/3 protein level also suggested that Nepeta bracteata Benth. extract could inhibit TGF-β mediated airway remodelling as well. Taken together, these data suggested that Nepeta bracteata Benth. may be a novel candidate for future antiasthma drug development.

  4. High-quality draft genome sequence of Rhizobium mesoamericanum strain STM6155, a Mimosa pudica microsymbiont from New Caledonia

    National Research Council Canada - National Science Library

    Klonowska, Agnieszka; López-López, Aline; Moulin, Lionel; Ardley, Julie; Gollagher, Margaret; Marinova, Dora; Tian, Rui; Huntemann, Marcel; Reddy, T.B.K; Varghese, Neha; Woyke, Tanja; Markowitz, Victor; Ivanova, Natalia; Seshadri, Rekha; Baeshen, Mohamed N; Baeshen, Nabih A; Kyrpides, Nikos; Reeve, Wayne

    2017-01-01

      Rhizobium mesoamericanum STM6155 (INSCD = ATYY01000000) is an aerobic, motile, Gram-negative, non-spore-forming rod that can exist as a soil saprophyte or as an effective nitrogen fixing microsymbiont of the legume Mimosa pudica L...

  5. Pharmacological evaluation of antihypertensive effect of aerial parts of Thymus linearis benth.

    Science.gov (United States)

    Alamgeer; Akhtar, Muhammad Shoaib; Jabeen, Qaiser; Khan, Hafeez Ullah; Maheen, Safirah; Haroon-Ur-Rash; Karim, Sabeha; Rasool, Shahid; Malik, Muhammad Nasir Hayat; Khan, Kifayatullah; Mushtaq, Muhammad Naveed; Latif, Fouzia; Tabassum, Nazia; Khan, Abdul Qayyum; Ahsan, Haseeb; Khan, Wasim

    2014-01-01

    Traditionally Thymus linearis Benth. have been used for treatment of various diseases including hypertension. The present study was conducted to evaluate the hypotensive and antihypertensive effect of aqueous methanolic extract of aerial parts of Thymus linearis Benth. in normotensive and hypertensive rats. Acute and subchronic studies were also conducted. The aqueous methanolic extract produced a significant decrease in SBP, DBP, MBP and heart rate of both normotensive and hypertensive rats. LDv, of the extract was found to be 3000 mg/kg. The extract also exhibited a reduction in serum ALT, AST, ALP, cholesterol, triglycerides and LDL levels, while a significant increase in HDL level was observed. It is conceivable therefore, that Thymus linearis Benth. contains certain active compound(s) that are possibly responsible for the observed antihypertensive activity. Moreover, these findings further authenticate the traditional use of this plant in folklore medicine.

  6. Terpenes From the Root of Salvia hypoleuca Benth

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    Saeidnia Soodabeh

    2012-10-01

    Full Text Available Abstract Background The genus Salvia, with nearly 900 species, is one of the largest members of Lamiaceae family. In the Flora of Iran, the genus Salvia is represented by 58 species of which 17 species are endemic. Salvia hypoleuca Benth., is one of these species growing wildly in northern and central parts of Iran. Salvia species are well known in folk medicine and widely used for therapeutic purposes. Literature review shows that there is no report on phytochemical investigation of the roots of S. hypoleuca. Results The separation and purification process were carried out using various chromatographic methods. Structural elucidation was on the basis of NMR and MS data, in comparison with those reported in the literature. The isolated compounds were identified as sitosteryl oleate (1, β-sitosterol (2, stigmasterol (3, manool (4, 7α-acetoxy royleanone (5, ursolic acid (6, oleanolic acid (7, 3-epicorosolic acid (8, 3-epimaslinic acid (9 and coleonolic acid (10. Conclusions In the present study, three sterols, two diterpenes and five triterpenes were isolated from the ethyl acetate extract of the roots of S. hypoleuca. As the chemotaxonomic significance, some of the isolated compounds (1–7, 9 have not been previously reported from the species S. hypoleuca, while the triterpenes 8 and 10 are now documented from Salvia genus for the first time.

  7. Terpenes From the Root of Salvia Hypoleuca Benth

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    Ahmad R Gohari

    2012-10-01

    Full Text Available Background:The genus Salvia, with nearly 900 species, is one of the largest members of Lamiaceae family. In the Flora of Iran, the genus Salvia is represented by 58 species of which 17 species are endemic. Salvia hypoleuca Benth., is one of these species growing wildly in northern and central parts of Iran. Salvia species are well known in folk medicine and widely used for therapeutic purposes. Literature review shows that there is no report on phytochemical investigation of the roots of S. hypoleuca.Results:The separation and purification process were carried out using various chromatographic methods. Structural elucidation was on the basis of NMR and MS data, in comparison with those reported in the literature. The isolated compounds were identified as sitosteryl oleate (1, β-sitosterol (2, stigmasterol (3, manool (4, 7α-acetoxy royleanone (5, ursolic acid (6,oleanolic acid (7, 3-epicorosolic acid (8, 3-epimaslinic acid (9 and coleonolic acid (10.Conclusions:In the present study, three sterols, two diterpenes and five triterpenes were isolated from the ethyl acetate extract of the roots of S. hypoleuca. As the chemotaxonomic significance, someof the isolated compounds (1–7, 9 have not been previously reported from the species S.hypoleuca, while the triterpenes 8 and 10 are now documented from Salvia genus for the first time.

  8. STUDIUL COMPLEX Al ULEIULUI VOLATIL DIN SPECIA PEROVSCHIA ATRIPLICIFOLIA BENTH.

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    Maricica COLŢUN

    2017-07-01

    Full Text Available În lucrare sunt prezentate rezultatele unui studiu vast privind biologia, compoziția chimică, fitochimia cantitativă și calitativă a uleiului volatil din specia Perovskia atriplicifolia, care se comportă ca plantă perenă, erbacee din familia Lamiaceae. În R. Moldova nu este cercetată ca plantă aromatică şi medicinală. Are o plasticitate ecologică mare, nu are cerinţe deosebite faţă de factorii climaterici. Se utilizează pentru proprietăţile sale febrifuge, în medicină – pentru scăderea febrei, deține proprietăți culinare dar şi decorative ori de purificare a aerului.THE COMPLEX STUDY OF THE ESSENTIAL OIL OF THE SPECIESPEROVSCHIA ATRIPLICIFOLIA BENTH.The paper presents the results of a vast study on the biology, chemical composition and qualitative phytochemistry of the essential oil of the species Perovskia atriplicifolia, which behaves like a perennial, herbaceous plant in the Lamiaceae family. In theRepublic ofMoldova, it has not been investigated as an aromatic and medicinal plant. It has a high ecological plasticity and no special requirements to climatic factors. It has febrifuge properties and is used in medicine to reduce fever. Besides, this plant is used for culinary, ornamental purposes and for air purification.

  9. Treatment with aqueous extract from Croton cajucara Benth reduces hepatic oxidative stress in streptozotocin-diabetic rats.

    Science.gov (United States)

    Rodrigues, Graziella Ramos; Di Naso, Fábio Cangeri; Porawski, Marilene; Marcolin, Eder; Kretzmann, Nélson Alexandre; Ferraz, Alexandre de Barros Falcão; Richter, Marc Francois; Marroni, Cláudio Augusto; Marroni, Norma Possa

    2012-01-01

    Croton cajucara Benth is a plant found in Amazonia, Brazil and the bark and leaf infusion of this plant have been popularly used to treat diabetes and hepatic disorders. The present study was designed to evaluate the oxidative stress as well as the therapeutic effect of Croton cajucara Benth (1.5 mL of the C. cajucara extract i.g.) in rats with streptozotocin-induced diabetes. Croton cajucara Benth was tested as an aqueous extract for its phytochemical composition, and its antioxidant activity in vitro was also evaluated. Lipid peroxidation and superoxide dismutase, catalase, and glutathione reductase activities were measured in the hepatic tissue, as well as the presence activation of p65 (NF-κB), through western blot. Phytochemical screening of Croton cajucara Benth detected the presence of flavonoids, coumarins and alkaloids. The extract exhibited a significant antioxidant activity in the DPPH-scavenging and the hypoxanthine/xanthine oxidase assays. Liver lipid peroxidation increased in diabetic animals followed by a reduction in the Croton-cajucara-Benth-treated group. There was activation of p65 nuclear expression in the diabetic animals, which was attenuated in the animals receiving the Croton cajucara Benth aqueous extract. The liver tissue in diabetic rats showed oxidative alterations related to the streptozotocin treatment. In conclusion the Croton cajucara Benth aqueus extract treatment effectively reduced the oxidative stress and contributed to tissue recovery.

  10. ABEJAS VISITANTES DE Mimosa pigra L. (MIMOSACEAE: COMPORTAMIENTO DE PECOREO Y CARGAS POLÍNICAS Bees visiting Mimosa pigra L. (Mimosaceae: foraging behavior and pollen loads

    Directory of Open Access Journals (Sweden)

    CLARA ISABEL AGUILAR SIERRA

    Full Text Available Se estudiaron las cargas polínicas de 13 taxones de abejas capturadas visitando a Mimosa pigra en la zona de influencia del Embalse Porce II (Antioquia, Colombia. De los 21 tipos polínicos encontrados, M. pigra presenta el mayor porcentaje acumulado de colecta; Mimosa pudica, Piper aduncum, Solanum diversifolium, Warszewiczia coccinea y Psidium guajava, en su orden, se pueden considerar fuentes alternativas de polen, para varias de las especies de abejas cuyas cargas polínicas fueron analizadas. Dentro de las especies de abejas capturadas visitando a M. pigra se pueden diferenciar varios grupos según el tipo y abundancia relativa de los tipos polínicos encontrados en sus cargas. Uno de ellos, incluye a siete especies de abejas con más del 85% de granos de polen de M. pigra; otro, con cuatro especies de abejas que colectaron más del 94,5% del polen en M. pigra y M. pudica. Adicionalmente, se encontraron especies como Trigona dorsalis con cargas de M. pigra (59,4%, de S. diversifolium (37,8% y especies de abejas como Lasioglossum sp. 113, en cuyas cargas polínicas predominan los granos de polen de P. aduncum (61,8% y de W.coccinea (36,4%, en contraste con los de M. pigra (1,3%. En cuanto a la riqueza de tipos polínicos colectados por las abejas sobresalen Trigona muzoensis (12 tipos polínicos y T. dorsalis (10 tipos, lo cual refleja nichos tróficos más amplios para estas especies y deja duda sobre su constancia floral o hábitos de limpieza.We studied the pollen loads of 13 taxa of wild bees visiting the flowers of Mimosa pigra on the influence zone of the Dam Project Porce II (Antioquia, Colombia. Out of 21 different pollen types, M. pigra represents the higuest percentage; Mimosa pudica, Piper aduncum, Solanum diversifolium, Warszewiczia coccinea and Psidium guajava, in that order, were also abundant, and are alternative sources of pollen for the different kinds of bees recorded. Among the species of bees collected, we differentiate

  11. Biosythesis of Silver Nanoparticles using Putri Malu (Mimosa pudica Leaves Extract and Microwave Irradiation Method

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    Is Fatimah

    2016-11-01

    Full Text Available In this paper, the biosynthesis of silver nanoparticles (AgNPs using Mimosa pudica extract is discussed. Mimosa pudica leaves extract using water as solvent was used as bio-reductor to an aqueous solution of silver nitrate (AgNO3 and in order to accelerate the reduction, microwave irradiation method was applied. The AgNPs obtained were characterized using UV-Vis spectrophotometry, FTIR spectrophotometry, XRD, SEM-EDX, and particle size analysis based on dynamic scattering method. Effect of preparation method to the formation of AgNPs is also evaluated in antibacterial activity towards E.coli and P. aeruginosa. Rapid and ecofriendly biosynthesis of stable silver nanoparticles was observed in this study. The characterization results and antibacterial assay indicated the uniform and smaller particle size of AgNPs obtained by using microwave method and positively enhance the antibacterial activity against tested bacteria.

  12. Jurema-Preta (Mimosa tenuiflora [Willd.] Poir.: a review of its traditional use, phytochemistry and pharmacology

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    Rafael Sampaio Octaviano de Souza

    2008-10-01

    Full Text Available Numerous plant species are used throughout the world to achieve the modified states of conscientiousness. Some of them have been used for the therapeutic purposes, such as Mimosa tenuiflora (Willd Poir. (family Mimosaceae known as "jurema-preta", an hallucinogenic plant traditionally used for curing and divination by the Indians of northeastern Brazil. In this review, several aspects of the use, phytochemistry, and pharmacology of this plant are considered.Numerosas espécies de plantas são usadas para alterar estados de consciência. Algumas são utilizadas para fins terapêuticos, como Mimosa tenuiflora (Willd Poir. (Mimosaceae conhecida como "jurema-preta", uma planta alucinógena, tradicionalmente utilizada pelos índios no nordeste do Brasil. Nesta revisão, são considerados diversos aspectos do uso, fitoquímica e farmacologia desta planta.

  13. Perilaku Krom Dalam Limbah Cair Penyamakan Kombinasi Krom-Gambir dan Krom-Mimosa Pada Penyamakan Kulit

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    Ardinal Ardinal

    2014-06-01

    Full Text Available Normal 0 false false false EN-US X-NONE X-NONE This study aims to determine the amount of chrome that come out together with tannery waste in a tanned combination process of chrome-gambier and chrome-mimosa. the process research performed by 2 stages. The 1st stage  using chrome with 5 concentration,    they were 2,4,6 and 8 %. Then it followed by the 2nd stage  tanning process by using vegetable tanning, gambier and mimosa, with each 7% and 9% concentration. The results showed that the combination of chrome-gambier tanned at the same concentration disposed chromium waste less than the combination of chrome-mimosa tanned. The lowest total chrome waste on the chrome-gambier tanning combination was 3.9 ppm at  2% chromium and 7% gambier concentration and the highest was 146.6 ppm at 8% chromium and 9% gambier concentrations.  The lowest total chrome waste on the combination of chrome-mimosa tanning was 2.2 ppm at  2% chromium and 7% mimosa concentration and the highest was 170.4 ppm at 8 % chromium and 9% mimosa concentration. The 2ndtanning, was combination tanning process, chrome-gambier able to reduce chromium levels more than the chrome-mimosa tanning.ABSTRAKPenelitian ini bertujuan untuk mengetahui jumlah krom yang ikut terbuang bersama limbah proses penyamakan kulit yang di samak kombinasi krom-gambir dan krom-mimosa. Pelaksanaan penelitian proses penyamakan kulit  dilakukan dengan 2 tahap. Tahap I menggunakan krom dengan 5 variasi  konsentrasi yaitu 2, 4, 6 dan 8%. Setelah penyamakan tahap I kemudian dilanjutkan penyamakan tahap II dengan menggunakan penyamakan nabati, gambir dan mimosa, dengan variasi konsentrasi masing-masing 7 dan 9%. Hasil penelitian menunjukkan bahwa perlakuan penyamakan kombinasi krom-gambir pada konsentrasi yang sama menghasilkan limbah krom yang lebih sedikit dibandingkan dengan samak kombinasi krom-mimosa. Jumlah limbah krom pada penyamak kombinasi krom-gambir terendah adalah 3,9 ppm pada konsentrasi krom

  14. Potensi Anthelmintik Akar Tanaman Putri Malu (Mimosa pudica L.) terhadap Hymenolepis nana pada Mencit

    OpenAIRE

    A. A. Candra; Ridwan, Y.; E. B. Retnani

    2008-01-01

    The objective of this experiment was to observe the anthelmintic effect of different concentration of root extract of the sensitive plant (Mimosa pudica L.) to Hymenolepis nana (Hymenolepis sp.) in mice. Sixty mice were divided into 6 groups consisting of 10 mice per group. Mice were infected with 100 infective eggs of Hymenolepis sp. after deworming by mebendazol. After reaching the prepaten phase ( 21st day), mice were treated with different concentration of root extract per oral, namely 1...

  15. Arbuscular mycorrhizal fungi in Mimosa tenuiflora (Willd.) Poir from Brazilian semi-arid

    OpenAIRE

    Tancredo Augusto Feitosa de Souza; Susana Rodriguez-Echeverría; Leonaldo Alves de Andrade; Helena Freitas

    2016-01-01

    Abstract Many plant species from Brazilian semi-arid present arbuscular mycorrhizal fungi (AMF) in their rhizosphere. These microorganisms play a key role in the establishment, growth, survival of plants and protection against drought, pathogenic fungi and nematodes. This study presents a quantitative analysis of the AMF species associated with Mimosa tenuiflora, an important native plant of the Caatinga flora. AMF diversity, spore abundance and root colonization were estimated in seven sampl...

  16. Jurema-Preta (Mimosa tenuiflora [Willd.] Poir.): a review of its traditional use, phytochemistry and pharmacology

    OpenAIRE

    Rafael Sampaio Octaviano de Souza; Ulysses Paulino de Albuquerque; Júlio Marcelino Monteiro; Elba Lúcia Cavalcanti de Amorim

    2008-01-01

    Numerous plant species are used throughout the world to achieve the modified states of conscientiousness. Some of them have been used for the therapeutic purposes, such as Mimosa tenuiflora (Willd) Poir. (family Mimosaceae) known as "jurema-preta", an hallucinogenic plant traditionally used for curing and divination by the Indians of northeastern Brazil. In this review, several aspects of the use, phytochemistry, and pharmacology of this plant are considered.Numerosas espécies de plantas são ...

  17. Antiprotozoal Activity of Flavonoids Isolated from Mimosa tenuiflora (Fabaceae-Mimosoideae)

    OpenAIRE

    Elihú Bautista; Fernando Calzada; Alfredo Ortega; Lilian Yépez-Mulia

    2011-01-01

    As result of the chemical study of the leaves and flowers of Mimosa tenuiflora (Willd.) Poir. (Fabaceae-Mimosoideae) eigth flavonoids were isolated: 6-methoxy-4¿-O-methylnaringenin (1), santin (2), 6-methoxynaringenin (3), tenuiflorin A (4), 5, 7, 4¿-trihydroxy-3, 6-dimethoxyflavone (5), 6-demethoxy-4¿-O-methylcapilarisine (6), 6- methoxykaempferol (7) and tenuiflorin C (8). Antiprotozoal activity of these compounds as well as the tenuiflorina B (9) and 6-desmethoxycapilarisine (10), isolated...

  18. Neutralizing Effects of Mimosa tenuiflora Extracts against Inflammation Caused by Tityus serrulatus Scorpion Venom

    OpenAIRE

    Mariana Angélica Oliveira Bitencourt; Maira Conceição Jerônimo de Souza Lima; Manoela Torres-Rêgo; Júlia Morais Fernandes; Arnóbio Antônio da Silva-Júnior; Denise Vilarinho Tambourgi; Silvana Maria Zucolotto; Matheus de Freitas Fernandes-Pedrosa

    2014-01-01

    Scorpion bite represents a significant and serious public health problem in certain regions of Brazil, as well as in other parts of the world. Inflammatory mediators are thought to be involved in the systemic and local immune response induced by Tityus serrulatus scorpion envenomation. The aim of this study was to evaluate the effect of extracts of Mimosa tenuiflora on model envenomation. In mice, the envenomation model is induced by Tityus serrulatus venom. Previous treatment of mice with fr...

  19. Jurema-Preta (Mimosa tenuiflora [Willd.] Poir.): a review of its traditional use, phytochemistry and pharmacology

    OpenAIRE

    Rafael Sampaio Octaviano de Souza; Ulysses Paulino Albuquerque; Júlio Marcelino Monteiro; Elba Lúcia Cavalcanti de Amorim

    2008-01-01

    Numerous plant species are used throughout the world to achieve the modified states of conscientiousness. Some of them have been used for the therapeutic purposes, such as Mimosa tenuiflora (Willd) Poir. (family Mimosaceae) known as "jurema-preta", an hallucinogenic plant traditionally used for curing and divination by the Indians of northeastern Brazil. In this review, several aspects of the use, phytochemistry, and pharmacology of this plant are considered.Numerosas espécies de plantas são ...

  20. Burkholderia spp. are the most competitive symbionts of Mimosa, particularly under N-limited conditions.

    Science.gov (United States)

    Elliott, Geoffrey N; Chou, Jui-Hsing; Chen, Wen-Ming; Bloemberg, Guido V; Bontemps, Cyril; Martínez-Romero, Esperanza; Velázquez, Encarna; Young, J Peter W; Sprent, Janet I; James, Euan K

    2009-04-01

    Bacteria isolated from Mimosa nodules in Taiwan, Papua New Guinea, Mexico and Puerto Rico were identified as belonging to either the alpha- or beta-proteobacteria. The beta-proteobacterial Burkholderia and Cupriavidus strains formed effective symbioses with the common invasive species Mimosa diplotricha, M. pigra and M. pudica, but the alpha-proteobacterial Rhizobium etli and R. tropici strains produced a range of symbiotic phenotypes from no nodulation through ineffective to effective nodulation, depending on Mimosa species. Competition studies were performed between three of the alpha-proteobacteria (R. etli TJ167, R. tropici NGR181 and UPRM8021) and two of the beta-rhizobial symbionts (Burkholderia mimosarum PAS44 and Cupriavidus taiwanensis LMG19424) for nodulation of these invasive Mimosa species. Under flooded conditions, B. mimosarum PAS44 out-competed LMG19424 and all three alpha-proteobacteria to the point of exclusion. This advantage was not explained by initial inoculum levels, rates of bacterial growth, rhizobia-rhizobia growth inhibition or individual nodulation rate. However, the competitive domination of PAS44 over LMG19424 was reduced in the presence of nitrate for all three plant hosts. The largest significant effect was for M. pudica, in which LMG19424 formed 57% of the nodules in the presence of 0.5 mM potassium nitrate. In this host, ammonium also had a similar, but lesser, effect. Comparable results were also found using an N-containing soil mixture, and environmental N levels are therefore suggested as a factor in the competitive success of the bacterial symbiont in vivo.

  1. An Analysis of Mimosa pudica Leaves Movement by Using LoggerPro Software

    Science.gov (United States)

    Sugito; Susilo; Handayani, L.; Marwoto, P.

    2016-08-01

    The unique phenomena of Mimosa pudica are the closing and opening movements of its leaves when they got a stimulus. By using certain software, these movements can be drawn into graphic that can be analysed. The LoggerPro provides facilities needed to analyse recorded videos of the plant's reaction to stimulus. Then, through the resulted graph, analysis of some variables can be carried out. The result showed that the plant's movement fits an equation of y = mx + c.

  2. Neutralisation of lethality, myotoxicity and toxic enzymes of Naja kaouthia venom by Mimosa pudica root extracts.

    Science.gov (United States)

    Mahanta, M; Mukherjee, A K

    2001-04-01

    Aqueous and alcoholic extracts of dried roots of Mimosa pudica were tested for their inhibitory activity on lethality, myotoxicity and toxic enzymes of Naja kaouthia venom. The aqueous extract, particularly the normal water extract, displayed a significant inhibitory effect on the lethality, myotoxicity and tested enzyme activities of venom compared with alcoholic extracts. The present finding suggests that aqueous extracts of M. pudica root possess compound(s), which inhibit the activity of cobra venom.

  3. Phylogenetic relationships and diversity of β-rhizobia associated with Mimosa species grown in Sishuangbanna, China.

    Science.gov (United States)

    Liu, Xiao Yun; Wu, Wei; Wang, En Tao; Zhang, Bin; Macdermott, Jomo; Chen, Wen Xin

    2011-02-01

    In order to investigate the genetic diversity of rhizobia associated with various exotic and invasive species in tropical mainland China, 116 bacterial isolates were obtained from Mimosa root nodules collected from Sishuangbanna and Yuanjiang districts of Yunnan province. Isolated rhizobia were characterized by RFLP analysis of 16S rRNA genes, SDS-PAGE of whole-cell proteins and BOX-PCR. Most of the isolated strains were identified as β-rhizobia belonging to diverse populations of Burkholderia and Cupriavidus, and the phylogenetic relationships of their 16S rRNA gene sequences showed that they were closely related to one of four β-rhizobia species: Burkholderia phymatum, B. mimosarum, B. caribensis or Cupriavidus taiwanensis. Additionally, among the 116 isolates, 53 different whole-cell SDS-PAGE profiles and 30 distinct BOX-PCR genotypic patterns were detected, which demonstrated the genetic and phenotypic diversity found within these Burkholderia and Cupriavidus strains. To the best of our knowledge, this is the first report that β-rhizobia are extant and possibly widespread on the Chinese mainland and nodulate easily with Mimosa plants. We also find it especially interesting that this appears to be the first report from mainland China of Cupriavidus symbionts of Mimosa. These records enrich our knowledge and understanding of the geographical distribution and diversity of these bacteria.

  4. Circadian variations in biologically closed electrochemical circuits in Aloe vera and Mimosa pudica.

    Science.gov (United States)

    Volkov, Alexander G; Baker, Kara; Foster, Justin C; Clemmons, Jacqueline; Jovanov, Emil; Markin, Vladislav S

    2011-04-01

    The circadian clock regulates a wide range of electrophysiological and developmental processes in plants. This paper presents, for the first time, the direct influence of a circadian clock on biologically closed electrochemical circuits in vivo. Here we show circadian variation of the plant responses to electrical stimulation. The biologically closed electrochemical circuits in the leaves of Aloe vera and Mimosa pudica, which regulate their physiology, were analyzed using the charge stimulation method. The electrostimulation was provided with different timing and different voltages. Resistance between Ag/AgCl electrodes in the leaf of Aloe vera was higher during the day than at night. Discharge of the capacitor in Aloe vera at night was faster than during the day. Discharge of the capacitor in a pulvinus of Mimosa pudica was faster during the day. The biologically closed electrical circuits with voltage gated ion channels in Mimosa pudica are also activated the next day, even in the darkness. These results show that the circadian clock can be maintained endogenously and has electrochemical oscillators, which can activate ion channels in biologically closed electrochemical circuits. We present the equivalent electrical circuits in both plants and their circadian variation to explain the experimental data. Copyright © 2011 Elsevier B.V. All rights reserved.

  5. Mimosa pudica seed mucilage: isolation; characterization and evaluation as tablet disintegrant and binder.

    Science.gov (United States)

    Ahuja, Munish; Kumar, Ashok; Yadav, Parvinder; Singh, Kuldeep

    2013-06-01

    In the present study Mimosa pudica seed mucilage was isolated, characterized and evaluated as tablet binder and disintegrant. Several properties of mucilage like high swelling index and gelling nature prompted us to explore its applications as disintegrating and binding agent. Disintegrant properties were evaluated by formulating directly compressed hydrochlorothiazide tablets containing 1%-10% (w/w) of seed mucilage as disintegrant and compared with the standard disintegrants. The disintegration time of mucilage containing tablets was found to be in the order of 3%>1%>5%>7.5%>10%. On comparative evaluation with standard disintegrants, it was observed that the order of disintegration of tablets was Ac-Di-SolMimosa mucilage at 6%, 8%, and 10% (w/w) concentration as the binder and compared with tablets prepared using PVP-K25 (1.7%, w/w) and acacia (6.8%, w/w) as the binder. Mimosa mucilage at 10% (w/w) concentration provided tablets with adequate hardness and friability. In conclusion, M. pudica seed mucilage is a potential tablet disintegrant and binder. Copyright © 2013 Elsevier B.V. All rights reserved.

  6. Antibacterial activities of the extracts of Mimosa pudica L. an in-vitro study

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    Nguyen Thi Le Thoa

    2015-01-01

    Full Text Available Mimosa pudica L., also called sensitive plant or touch-me-not, belongs to the genus Mimosa (Family: Mimosaceae. This plant grows as a weed in nearly every parts of Vietnam and is used as a traditional medicine for the treatment of some diseases. This study aims to evaluate the antibacterial activity of the water and ethanol extracts of this plant by using disc diffusion method. The total flavonoid as quercetin equivalent (QE per gram (dry weight of these two extracts was also estimated. The result of tests for in-vitro antibacterial activity indicates that the ethanol extract showed significant activity against E.coli, S.aureus, B.subtilis and S.typhi with the zone of inhibition was 11mm, 19mm, 17mm and 16mm respectively. The water extract only inhibited the growth of S.aureus (14mm and B.subtilis (15mm and there was no resistance against E.coli and S.typhi. The analysis of total flavonoid content found that the ethanol extract contains higher amount of flavonoid than water extract and flavonoid is responsible mainly for the antibacterial activity of Mimosa pudica L.

  7. Diuretic Activity of Ethanolic Root Extract of Mimosa Pudica in Albino Rats.

    Science.gov (United States)

    Hl, Kalabharathi; Sl, Shruthi; Ps, Vaibhavi; Vh, Pushpa; Am, Satish; Sibgatullah, Mohammad

    2015-12-01

    Diuretics are the drugs which increase the urine output. This property is useful in various pathological conditions of fluid overload. The presently available diuretics have lot of adverse effects. Our study has evaluated the diuretic activity of ethanolic root extract of Mimosa pudica as an alternative/new drug which may induce diuresis. To evaluate the diuretic activity of ethanolic root extract of Mimosa pudicaa in albino rats. Ethanolic root extract of Mimosa pudica (EEMP) was prepared using soxhlet's apparatus. Albino rats were divided into 5 groups of 6 rats each. Group-I (Control) received distilled water 25ml/kg orally. Group-II (Standard) received Furosemide 20mg/kg orally. Group-III received EEMP 100 mg/kg, Group-IV received EEMP 200 mg/kg and Group-V received EEMP 400 mg/kg. The urine samples were collected for all the groups upto 5 hours after dosing and urine volume was measured. Urine was analysed for electrolytes (Na+, K+ and Cl-). ANOVA, Dunnet's test and p-values were measured and data was analysed. EEMP exhibited significant diuretic activity by increasing urine volume and also by enhancing elimination of Sodium (Na+), Potassium (K+) and Chloride (Cl-) at doses of 100 and 200mg/kg. EEMP possesses significant diuretic activity and has a beneficial role in volume overload conditions.

  8. New adduct of abietane-type diterpene from Salvia leriifolia Benth.

    Science.gov (United States)

    Hussain, Amjad; Adhikari, Achyut; Iqbal Choudhary, M; Ayatollahi, Syed Abdulmajid; Atta-Ur-Rahman

    2016-07-01

    A new adduct of abietane-type diterpene, salvialeriicone (1), was isolated from Salvia leriifolia Benth., along with a new chemical entity nor-abietane diterpene, 2-isopropyl-8,8-dimethyl-7,8-dihydrophenanthrene-1,4,5(6H)-trione (2). Their structures were determined using mass spectrometry, and 1D- and 2D-NMR spectroscopy.

  9. Cultivos celulares de Choibá Dipteryx oleifera Benth

    Directory of Open Access Journals (Sweden)

    Paola Andrea Murillo Gómez

    2013-12-01

    Full Text Available Título en ingles: Cell cultures of Choiba Dipteryx oleifera BenthTítulo corto: Cultivos celulares de ChoibáResumen: Choibá (Dipteryx oleifera Benth es un árbol de la familia Fabaceae (Papilionoideae, con una distribución geográfica reportada desde Nicaragua  hasta Colombia a una altura de hasta 1000 msnm. Crece en bosque húmedo, muy húmedo o premontano húmedo. Esta especie es considerada vulnerable debido a la sobreexplotación de su madera, ya que es un árbol altamente apetecido por esta y por sus frutos. Su almendra almacena una buena cantidad de aceites con potencial para la industria alimentaria, lo que podría resultar en una nueva fuente alimenticia, por lo cual el cultivo in vitro de vegetales con el propósito de producir compuestos de interés, marca un punto de partida para reducir el uso del suelo y lograr componentes bioactivos bajo condiciones controladas. En este trabajo, como una primera etapa experimental, se evaluó el crecimiento celular en suspensiones,  a partir de callo inducido en explantes de cotiledón; se ensayaron 6 tratamientos diferentes, la mitad de estos con MS como medio basal y la otra mitad con B5, cada uno de los dos grupos con un control y la combinación hormonal de 2.5 mg/L de 2,4-D y 1 mg/L de BAP o kinetina, suplementado con adenina, biotina, glutamina y ácido pantoténico y 30 g/L de sacarosa, bajo completa oscuridad. Se encontró que dos tratamientos con MS en combinación con 2.5 mg/L de 2,4-D y 1 mg/L de kinetina o BAP fueron los mejores.Palabras clave: Biomasa, choibá, cultivos celulares, Dipteryx oleifera.Abstract: Choibá (Dipteryx olifera is a tree of the Fabaceae family, with a geographical distribution reported from Nicaragua to Colombia, nearly 1.000 msnm in a tropical rain forest. This species is a highlydesiredtreefor its timber andfruits, thekernelstore alot ofimportant oilsfor the food industry, resulting in a new possible food source,so we are making in vitro cultivation

  10. Sexo para quase todos: a prostituição feminina na Vila Mimosa Sex for almost all: female prostitution in Vila Mimosa

    Directory of Open Access Journals (Sweden)

    Elisiane Pasini

    2005-12-01

    Full Text Available Este artigo tem como base de análise uma pesquisa etnográfica localizada numa antiga zona de prostituição feminina fechada na cidade do Rio de Janeiro - a Vila Mimosa. Aqui apresento a problemática de que espaços criados em função do consumo de sexo local também estão sendo voltados para o encontro entre trabalhadoras/es do sexo locais e estrangeiros. A discussão centra-se em como este macro espaço de prostituição feminina transforma-se num palco de conflito quando se torna um mercado sexual também utilizado pelos estrangeiros.This article is based on ethnographic research conducted in an old zone of closed female prostitution in Rio de Janeiro - Vila Mimosa. I argue that the spaces created for the consumption of local sex are being used also for encounters between local sex laborers and foreigners. The discussion rises the question that this macrospace of female prostitution is becoming a stage for conflict when it becomes also a sex market used by foreigners.

  11. Draft Genome Sequence of Rhizobium mesoamericanum STM3625, a Nitrogen-Fixing Symbiont of Mimosa pudica Isolated in French Guiana (South America).

    Science.gov (United States)

    Moulin, Lionel; Mornico, Damien; Melkonian, Rémy; Klonowska, Agnieszka

    2013-01-01

    Rhizobium mesoamericanum STM3625 is a Mimosa pudica symbiont isolated in French Guiana. This strain serves as a model bacterium for comparison of adaptation to mutualism (symbiotic traits, bacterial genetic programs for plant infection) between alpha and beta rhizobial symbionts of Mimosa pudica.

  12. Diverse bacteria isolated from root nodules of Trifolium, Crotalaria and Mimosa grown in the subtropical regions of China.

    Science.gov (United States)

    Liu, Xiao Yun; Wang, En Tao; Li, Ying; Chen, Wen Xin

    2007-07-01

    To analyze the diversity and relationships of rhizobia in the subtropical and tropical zones of China, we characterized 67 bacterial strains isolated from root nodules of five legume species in the genera Trifolium, Crotalaria and Mimosa . PCR-amplified 16S rDNA RFLP, numerical taxonomy, SDS-PAGE of whole cell proteins, sequencing of 16S rDNA and DNA-DNA hybridization grouped the isolates into 17 lineages belonging to Bradyrhizobium, Mesorhizobium, Rhizobium, Sinorhizobium and Burkholderia, as well as a non-symbiotic group of Agrobacterium. The Rhizobium group contained twenty strains isolated from Mimosa pudica, Crotalaria pallida and two species of Trifolium. Fifteen of them were R. leguminosarum. Twenty-one strains isolated from four species of Trifolium, Crotalaria and Mimosa were classified into five groups of Bradyrhizobium, including B. japonicum. Agrobacterium group composed of 20 isolates from Mimosa pudica, C. pallida and Trifolium fragiferum. In addition, several strains of Sinorhizobium and Mesorhizobium associated with Trifolium and Burkholderia associated with Mimosa pudica were also identified. The predominance of Bradyrhizobium in the nodules of Trifolium was a novel finding and it demonstrated that the nodule microsymbionts might be selected by both the geographic factors and the legume hosts.

  13. Burkholderia phymatum is a highly effective nitrogen-fixing symbiont of Mimosa spp. and fixes nitrogen ex planta.

    Science.gov (United States)

    Elliott, Geoffrey N; Chen, Wen-Ming; Chou, Jui-Hsing; Wang, Hui-Chun; Sheu, Shih-Yi; Perin, Liamara; Reis, Veronica M; Moulin, Lionel; Simon, Marcelo F; Bontemps, Cyril; Sutherland, Joan M; Bessi, Rosana; de Faria, Sergio M; Trinick, Michael J; Prescott, Alan R; Sprent, Janet I; James, Euan K

    2007-01-01

    * The ability of Burkholderia phymatum STM815 to effectively nodulate Mimosa spp., and to fix nitrogen ex planta, was compared with that of the known Mimosa symbiont Cupriavidus taiwanensis LMG19424. * Both strains were equally effective symbionts of M. pudica, but nodules formed by STM815 had greater nitrogenase activity. STM815 was shown to have a broader host range across the genus Mimosa than LMG19424, nodulating 30 out of 31 species, 21 of these effectively. LMG19424 effectively nodulated only nine species. GFP-marked variants were used to visualise symbiont presence within nodules. * STM815 gave significant acetylene reduction assay (ARA) activity in semisolid JMV medium ex planta, but no ARA activity was detected with LMG19424. 16S rDNA sequences of two isolates originally from Mimosa nodules in Papua New Guinea (NGR114 and NGR195A) identified them as Burkholderia phymatum also, with nodA, nodC and nifH genes of NGR195A identical to those of STM815. * B. phymatum is therefore an effective Mimosa symbiont with a broad host range, and is the first reported beta-rhizobial strain to fix nitrogen in free-living culture.

  14. Female sex pheromone secreted by Carmenta mimosa (Lepidoptera: Sesiidae), a biological control agent for an invasive weed in Vietnam.

    Science.gov (United States)

    Vang, Le Van; Khanh, Chau Nguyen Quoc; Shibasaki, Hiroshi; Ando, Tetsu

    2012-01-01

    Larvae of the clearwing moth, Carmenta mimosa (Lepidoptera: Sesiidae), bore into the trunk of Mimosa pigra L., which is one of the most invasive weeds in Vietnam. GC-EAD and GC-MS analyses of a pheromone gland extract revealed that the female moths produced (3Z,13Z)-3,13-octadecadienyl acetate. A lure baited with the synthetic acetate alone successfully attracted C. mimosa males in a field test. While the addition of a small amount of the corresponding alcohol did not strongly diminish the number of captured males, a trace of the aldehyde derivative or the (3E,13Z)-isomer markedly inhibited the attractiveness of the acetate. The diurnal males were mainly attracted from 6:00 am to 12:00 am.

  15. Proof that Burkholderia strains form effective symbioses with legumes: a study of novel Mimosa-nodulating strains from South America.

    Science.gov (United States)

    Chen, Wen-Ming; de Faria, Sergio M; Straliotto, Rosângela; Pitard, Rosa M; Simões-Araùjo, Jean L; Chou, Jui-Hsing; Chou, Yi-Ju; Barrios, Edmundo; Prescott, Alan R; Elliott, Geoffrey N; Sprent, Janet I; Young, J Peter W; James, Euan K

    2005-11-01

    Twenty Mimosa-nodulating bacterial strains from Brazil and Venezuela, together with eight reference Mimosa-nodulating rhizobial strains and two other beta-rhizobial strains, were examined by amplified rRNA gene restriction analysis. They fell into 16 patterns and formed a single cluster together with the known beta-rhizobia, Burkholderia caribensis, Burkholderia phymatum, and Burkholderia tuberum. The 16S rRNA gene sequences of 15 of the 20 strains were determined, and all were shown to belong to the genus Burkholderia; four distinct clusters could be discerned, with strains isolated from the same host species usually clustering very closely. Five of the strains (MAP3-5, Br3407, Br3454, Br3461, and Br3469) were selected for further studies of the symbiosis-related genes nodA, the NodD-dependent regulatory consensus sequences (nod box), and nifH. The nodA and nifH sequences were very close to each other and to those of B. phymatum STM815, B. caribensis TJ182, and Cupriavidus taiwanensis LMG19424 but were relatively distant from those of B. tuberum STM678. In addition to nodulating their original hosts, all five strains could also nodulate other Mimosa spp., and all produced nodules on Mimosa pudica that had nitrogenase (acetylene reduction) activities and structures typical of effective N2-fixing symbioses. Finally, both wild-type and green fluorescent protein-expressing transconjugant strains of Br3461 and MAP3-5 produced N2-fixing nodules on their original hosts, Mimosa bimucronata (Br3461) and Mimosa pigra (MAP3-5), and hence this confirms strongly that Burkholderia strains can form effective symbioses with legumes.

  16. Hepatics alterations and genotoxic effects of Croton cajucara Benth (SACACA in diabetic rats Alterações hepáticas e efeitos genotóxicos do Croton cajucara Benth (SACACA em ratos diabéticos

    Directory of Open Access Journals (Sweden)

    Graziella Rodrigues

    2010-09-01

    Full Text Available CONTEXT: Croton cajucara Benth is a plant found in Amazonia, Brazil and the bark and leaf infusions of this plant have been popularly used to treat diabetes and hepatic disorders. OBJECTIVES: This study investigated effects hepatics alterations and genotoxic and antidiabetic effect of Croton cajucara Benth bark extracts treatment in streptozotocin-induced diabetic rats. METHODS: Male Wistar rats were divided into six groups: control rats; control rats treated with Croton cajucara Benth extract during 5 and 20 days; diabetic rats, and diabetic rats treated with Croton cajucara Benth during 5 and 20 days. Diabetes was induced by a single intraperitoneal injection of streptozotocin (70 mg/kg. Eight weeks later we measured glucose, triglyceride, cholesterol and hepatic transaminases on blood. The bone marrow micronucleus assay was used to assess the genotoxic activity of Croton cajucara Benth. RESULTS: Treatment with aqueous extrat of Croton cajucara was able to significantly reduce levels of triglycerides in diabetic animals, however, did not modify significantly the levels of glucose and cholesterol in these animals. There was no significant elevation in liver transaminases in the control group treated with Croton cajucara Benth, as there was no genotoxic effect of treatment in this model. Our results did not show a significant effect on glucose and cholesterol reduction, the treatment was able to significantly reduce triclycerides plasmatic level. There was no significant alterations on hepatic transferase in the animals from the control group treated with Croton cajucara Benth. It was observed no genotoxic effect of the treatment in the model studied. CONCLUSION: In this study Croton cajucara bark extract showed absence of hepatotoxicity in this animal model and presented a hypolipidemic activity, and could be used to reverse dyslipidemia associated with diabetes and to prevent the cardiovascular complications that are very prevalent in diabetic

  17. Flavonolignoids and other compounds isolated from Mimosa artemisiana Heringer and Paula; Flavonoides e outros compostos isolados de Mimosa artemisiana Heringer e Paula

    Energy Technology Data Exchange (ETDEWEB)

    Nascimento, Ildomar Alves do [Universidade Federal Rural do Rio de Janeiro, Seropedica, RJ (Brazil). Dept. de Quimica; Braz-Filho, Raimundo; Mathias, Leda [Universidade Estadual do Norte Fluminense (UENF), Campos dos Goytacases, RJ (Brazil). Centro de Ciencias Tecnologicas. Lab. de Ciencias Quimicas; Carvalho, Mario Geraldo de [Universidade Federal do Rio de Janeiro (NPPN/UFRJ), RJ (Brazil). Centro de Ciencias da Saude. Nucleo de Pesquisa em Produtos Naturais; Fonseca, Fabio de Alcantara [Instituto Estadual de Florestas (IEF), Belo Horizonte, MG (Brazil)

    2012-07-01

    This the first phytochemical investigation of Mimosa artemisiana (Leguminosae-Mimosoideae) describing the isolation and identification of quercitrin, myricitrin, 3,5,4'-trihydroxy-6,7-dimethoxyflavone (6,7-dimethylkaempferol), flavolignans, 3-O-{beta}-D-glucopyranosyl sitosterol, lupeol, sitostenone, stigmastenone, campestenone, sitosterol, stigmasterol, campesterol, methyl indole-3-carboxylate and indole-3-carboxaldehyde in the extracts from the leaves and wood of this plant. This is the first registry of 6,7-dimethoxy,4'-hydroxy-flavone and the flavonolignans in this genera. The isolation of all metabolites was made by chromatographic methods and the structures were established on the basis of IR, MS, {sup 1}H and {sup 13}C NMR spectra analysis, comparison with literature data and GC-MS of mixtures analysis. (author)

  18. QUALIDADE DOS TANINOS DE JUREMA-PRETA (Mimosa tenuiflora PARA A PRODUÇÃO DE ADESIVO TANINO FORMALDEÍDO Mimosa tenuiflora

    Directory of Open Access Journals (Sweden)

    Tatiane Kelly Barbosa de Azevêdo

    2015-01-01

    Full Text Available ABSTRACTMimosa tenuiflorais an abundant species in the Brazilian semi-arid region which offers potential for thetannin production but need research indicating the best ways of its application. This study aimed to evaluatethe quality of tannin fromMimosa tenuiflorafor the formaldehyde tannin adhesive production with theplant collection period. The bark collections were carried out in the months of August and December of2008 and in April and August of 2009, in Lameirão farm, in municipality of Santa Terezinha, Paraíba state, Brazil, being collected 10 plants per period, totaling 40 plants. The barks were taken from the trunk andbranches up to 3.0 cm in diameter, ground into mill knives and classified in sieves, having been employedfor the extraction of tannins, particles less than 1.0 x 2.0 cm and superior than 0.2 x 0.2 cm. The extracttannic for the adhesives production was extracted in distilled water in an autoclave at boiling temperatureof water at atmospheric pressure, concentrated by evaporation and kept in an oven at 70 ± 3 °C until thecomplete evaporation of moisture, and was evaluated the total solid content, Stiasny’s index and condensedtannin content in the bark by collection period. To produce the adhesives, an acid sulphitation of tanninswas obtained. There was influence of period of collection of the plants in condensed tannin contents. Itwas found in adhesives produced with the tannins ofMimosa tenuiflora, solid content values, similar tothose ofAcacia mearnsii, no significant difference between collection periods for the solid content andgelatinization time of adhesives was obtained. However, the collection period influenced the pH andviscosity of adhesives. The adhesives produced showed high viscosity, indicating the need for research toimprove the adhesives produced.

  19. CHROMOSOME NUMBERS OF SPECIES OF MIMOSA (LEGUMINOSAE OF PARAGUAY NÚMEROS CROMOSÓMICOS DE ESPECIES DE MIMOSA (LEGUMINOSAE DE PARAGUAY

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    Guillermo Seijo

    2010-11-01

    Full Text Available

    In the present study the chromosome numbers of Mimosa xanthocentra subsp. subsericea var. subsericea, M. balansae, M. chacoensis, M. rojasiiall with 2n=26 and M. lupinoides with 2n=52 are reported for the first time. The 2n=52 of M. somnians subsp. viscida vara viscida and of M. somnians subsp. somnians var. somnians are new and differ from the 2n=26 published before for M. somnians var. somniansof Argentina. M. debiliswith 2n=26 also differs from the numbers
    reported previously for M. debilis var. debilis from Argentina with 2n=52. The 2n=26 of M. bimucronata var. adenocarpa coincides with the reported number for M. bimucronata var.
    bimucronata, and the number of M. polycarpa var. spegazzinii with 2n=26 is confirmed.
    En el presente estudio el número de cromosomas de la subsp Mimosa xanthocentra. subsericea var. subsericea, M. balansae, M. chacoensis, M. rojasii todos con 2n = 26 y M. lupinoides con 2n = 52 ha sido reportado en primer término. El 2n = 52 de M. somnians subsp. viscida var. viscida y de M. somnians subsp. somnians var. somnians son nuevas y diferentes de las 2n = 26 publicados antes para M. somnians var. somnians de Argentina. M. debilis con 2n = 26 también difiere de las cifras reportadas previamente para M. debilis var. debilis de Argentina con 2n = 52. El 2n = 26 de adenocarpa M. bimucronata var. coincide con la cifra reportada para M. bimucronata var. bimucronata, y el número de M. polycarpa var. spegazzinii con 2n = 26 se confirma

  20. The effect of indole acids on the germination of Mimosa bimucronata

    OpenAIRE

    2016-01-01

    Las semillas de Mimosa bimucronata (DC) OK poseen un letargo causado por la impermeabilidad de los tegumentos, que puede ser interrumpido permitiendo un 100% de germinación. Luego de la aplicación de ácidos indólicos hay respuesta, sea cual fuere el efecto en la regulación de la germinación. Aplicando ácido 3 indolacético, ácido indolbutírico y ácido indolpropiónico en las concentraciones de control, 10, 50, 100, 500 y 1000 ppm, se verifica un efecto inhibitorio a medida que aumentan las conc...

  1. Isolation and characterization of microsatellite markers from the acacia-ant Crematogaster mimosae.

    Science.gov (United States)

    Rubin, B E; Makarewich, C A; Talaba, A L; Stenzler, L; Bogdanowicz, S M; Lovette, I J

    2009-07-01

    We describe 10 microsatellite loci developed from Crematogaster mimosae, an ant species that nests mutualistically in Acacia drepanolobium trees in east Africa. Polymorphism ranged from 4 to 16 alleles per locus (mean = 7.3). Observed and expected heterozygosities ranged from 0.485 to 0.813 (mean 0.626), and from 0.502 to 0.894 (mean 0.674), respectively. These markers will foster studies of the population structure, colony structure, and reproductive strategies of these ants.

  2. Isolation of Fonsecaea pedrosoi from thorns of Mimosa pudica, a probable natural source of chromoblastomycosis.

    Science.gov (United States)

    Salgado, Claudio Guedes; da Silva, Jorge Pereira; Diniz, José Antônio Picanço; da Silva, Moisés Batista; da Costa, Patrícia Fagundes; Teixeira, Claudio; Salgado, Ubirajara Imbiriba

    2004-01-01

    We report the isolation of Fonsecaea pedrosoi from thorns of the plant Mimosa pudica L. at the place of infection identified by one of our patients. Clinical diagnosis of chromoblastomycosis was established by direct microscopic examination and cultures from the patient's lesion. The same species was isolated from the patient and from the plant. Scanning electron microscopy of the surface of the thorns showed the characteristic conidial arrangement of F. pedrosoi. These data indicate that M. pudica could be a natural source of infection for the fungus F. pedrosoi.

  3. Perbedaan Percepatan Penyembuhan Luka Bakar dari Ekstrak Kulit Buah Jengkol (Pithecellobium lobatum Benth.)

    OpenAIRE

    Darwin

    2011-01-01

    Dog fruit (Pithecellobium lobatum Benth.) is one of the herbs are efficacious. Rind dog fruit including waste in traditional markets and provide less economic value. Leaf dog fruit efficacious as eczema, scabies, sores and ulcers medicine, the bark as lowering blood sugar and rind can be used as a skin ulcer medication, insect repellent, burn. One of the chemical compounds from dog fruit rind is tannin. Serves as an astringent tannins that cause shrinkage pores of the skin, ...

  4. Post-harvest Treatment of the Pogostemon cablin Benth Oil Yield

    OpenAIRE

    2016-01-01

    Patchouli oil is one of the essential oils that are important in the perfume industry, cosmetics, and medicine. Patchouli oil is obtained from the distillation of the trunk, branches, and leaves of patchouli. One of the factors affecting the yield of patchouli oil is a post-harvest treatment. This study aims to determine the yield of patchouli (Pogostemon cablin Benth) oil from different processes in post-harvest management. There were two post-harvest treatments committed to patchouli leaves...

  5. DORMÊNCIA DE SEMENTES E PRODUÇÃO DE MUDAS DE Dimorphandra mollis Benth.

    OpenAIRE

    Mauro Vasconcelos Pacheco; Vilmar Luciano Mattei; Valderez Pontes Matos; Lúcia Helena de Moura Sena; Anna Gorett de Figueiredo Almeida Sales

    2011-01-01

    Dimorphandra mollis Benth. is an important native forest species coming from Cerrado and Caatinga due to its economical and ecological use, what justifies the existence of programs of seedling production. This paper aimed to study the efficient methodology to overcome dormancy in seeds and to evaluate, in natural nursery, the effects of distinct substrates and the fertilization on the initial growth of plants. The following treatments applied: T1 - control (seeds whithout scarification); T2 ¿...

  6. Two new phenylpropanoid glycosides with interesterification from Scrophularia dentata Royle ex Benth

    Science.gov (United States)

    Zhang, Liuqiang; Yang, Zhuo; Jia, Qi; Dorje, Gaawe; Zhao, Zhili; Guo, Fujiang; Li, Yiming

    2013-10-01

    Two new phenylpropanoid glycosides (1-2), along with seven known ones (3-9), were isolated from the whole plant of Scrophularia dentata Royle ex Benth. Their structures were elucidated by spectroscopic methods. Among them, compounds 1 and 2 failed to separated, because they can easily transform into each other by acyl migrant reaction. In this paper, the interesterification mechanism was discussed firstly and the rule can be used in the similar structure elucidation in future.

  7. BIOLOGIA DA REPRODUÇÃO EM QUATRO ESPÉCIES DE Centrosema (DC. BENTH

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    Alice Battistin

    1994-01-01

    Full Text Available RESUMO Com a finalidade de obter informações básicas sobre biologia floral das espécies: C. brasilianum (L. Benth., C. Virginianum (L Benth., C. shottii (Mill. K. Sch. e C. pascuorum (Mart. Benth., foram estudados comparativamente três parâmetros biológicos: número de óvulos por ovário, sementes por vagem que chegaram à maturidade e grãos de pólen viáveis. Os dados foram coletados no Campo Experimental do Instituto de Genética da ESALQ/USP - Piracicaba, SP e no Jardim Botânico do Departamento de Biologia da Universidade Federal de Santa Maria (UFSM - Santa Maria, RS. As diferenças ocorridas nos parâmetros analisados mostram, de uma certa forma, a individualidade comportamental de cada espécie, decorrente de uma sensibilidade e variabilidade específicas. Todas as espécies estudadas tiveram uma produção de óvulos férteis acima de 78% e pólen viável acima de 95%. que refletiu uma elevada porcentagem de sementes viáveis (com exceção de C. shottii e C. pascuorum - Santa Maria, RS, garantindo desta forma a perpetuação da espécie.

  8. Evaluation of anti-diarrhoeal potential of ethanolic extract of Mimosa pudica leaves

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    Md. Saifuddin Khalid

    2011-01-01

    Full Text Available Diarrhoea is a major public health problem in developing countries and is said to be endemic in many regions of Asia. It is a leading cause of high degree of morbidity and mortality. The anti-diarrhoeal potential of the ethanolic extract of leaves of Mimosa pudica Linn (Mimosaceae has been evaluated using several experimental models in Wistar albino rats. The ethanolic extract inhibited castor oil induced diarrhoea and PGE2 induced enteropooling in rats and has also reduced gastrointestinal motility after charcoal meal administration. The ethanolic extract at 200 and 400 mg/kg was showed significantly inhibited diarrhoea. There was a significant (P<0.001 dose-dependent decrease in the diarrhoea produced by all the three models in rats as compared to the standard drug. The anti-diarrhoeal property may be related to the tannin and flavonoids present in the extract. These results clearly indicated that ethanolic extract of the leaves of Mimosa pudica is effective against diarrhoeal disease

  9. Biosorption of Cu(II) from aqueous solutions by mimosa tannin gel

    Energy Technology Data Exchange (ETDEWEB)

    Sengil, I. Ayhan [Department of Environmental Engineering, Engineering Faculty, Sakarya University, 54100 Sakarya (Turkey)], E-mail: asengil@sakarya.edu.tr; Ozacar, Mahmut [Department of Chemistry, Science and Arts Faculty, Sakarya University, 54100 Sakarya (Turkey)

    2008-09-15

    The biosorption of Cu(II) from aqueous solutions by mimosa tannin resin (MTR) was investigated as a function of particle size, initial pH, contact time and initial metal ion concentration. The aim of this study was to understand the mechanisms that govern copper removal and find a suitable equilibrium isotherm and kinetic model for the copper removal in a batch reactor. The experimental isotherm data were analysed using the Langmuir, Freundlich and Temkin equations. The equilibrium data fit well in the Langmiur isotherm. The experimental data were analysed using four sorption kinetic models - the pseudo-first- and second-order equations, and the Elovich and the intraparticle diffusion equation - to determine the best fit equation for the biosorption of copper ions onto mimosa tannin resin. Results show that the pseudo-second-order equation provides the best correlation for the biosorption process, whereas the Elovich equation also fits the experimental data well. Thermodynamic parameters such as the entropy change, enthalpy change and Gibb's free energy change were found out to be 153.0 J mol{sup -1} K{sup -1}, 42.09 kJ mol{sup -1} and -2.47 kJ mol{sup -1}, respectively.

  10. Neutralizing Effects of Mimosa tenuiflora Extracts against Inflammation Caused by Tityus serrulatus Scorpion Venom

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    Mariana Angélica Oliveira Bitencourt

    2014-01-01

    Full Text Available Scorpion bite represents a significant and serious public health problem in certain regions of Brazil, as well as in other parts of the world. Inflammatory mediators are thought to be involved in the systemic and local immune response induced by Tityus serrulatus scorpion envenomation. The aim of this study was to evaluate the effect of extracts of Mimosa tenuiflora on model envenomation. In mice, the envenomation model is induced by Tityus serrulatus venom. Previous treatment of mice with fractions from M. tenuiflora was able to suppress the cell migration to the peritoneal cavity. The treatment of mice with M. tenuiflora extracts also decreased the levels of IL-6, IL-12, and IL-1β. We concluded that the administration of the extract and fractions resulted in a reduction in cell migration and showed a reduction in the level of proinflammatory cytokines. This study demonstrates, for the first time, the anti-inflammatory effect of aqueous extract from the Mimosa tenuiflora plant on T. serrulatus venom.

  11. ABEJAS VISITANTES DE Mimosa pigra L. (MIMOSACEAE:COMPORTAMIENTO DE PECOREO Y CARGAS POLÍNICAS

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    AGUILAR SIERRA CLARA ISABEL

    2009-04-01

    Full Text Available

    RESUMEN

    Se estudiaron las cargas polínicas de 13 taxones de abejas capturadas visitando a Mimosa pigra en la zona de influencia del Embalse Porce II (Antioquia, Colombia. De los 21 tipos polínicos encontrados, M. pigra presenta el mayor porcentaje acumulado de colecta; Mimosa pudica, Piper aduncum, Solanum diversifolium, Warszewiczia coccinea y Psidium guajava, en su orden, se pueden considerar fuentes alternativas de polen, para varias de las especies de abejas cuyas cargas polínicas fueron analizadas. Dentro de las especies de abejas capturadas visitando a M. pigra se pueden diferenciar varios grupos según el tipo y abundancia relativa de los tipos polínicos encontrados en sus cargas. Uno de ellos, incluye a siete especies de abejas con más del 85% de granos de polen de M. pigra; otro, con cuatro especies de abejas que colectaron más del 94,5% del polen en M. pigra y M. pudica. Adicionalmente, se encontraron especies como Trigona dorsalis con cargas de M. pigra (59,4%, de S. diversifolium (37,8% y especies de abejas como Lasioglossum sp. 113, en cuyas cargas polínicas predominan los granos de polen de P. aduncum (61,8% y de W.coccinea (36,4%, en contraste con los de M. pigra (1,3%. En cuanto a la riqueza de tipos polínicos colectados por las abejas sobresalen Trigona muzoensis (12 tipos polínicos y T. dorsalis (10 tipos, lo cual refleja nichos tróficos más amplios para estas especies y deja duda sobre su constancia floral o hábitos de limpieza.

    Efficacy evaluations of Mimosa pudica tannin isolate (MPT) for its anti-ophidian properties.

    Science.gov (United States)

    Ambikabothy, Jamunaa; Ibrahim, Halijah; Ambu, Stephen; Chakravarthi, Srikumar; Awang, Khalijah; Vejayan, Jaya

    2011-09-01

    Evaluations of the anti-snake venom efficacy of Mimosa pudica tannin isolate (MPT) obtained from root of the plant. MPT was investigated in vitro and in vivo for its efficacy against the venom of Naja kaouthia snake. In vitro: (1) mice injected i.p. with MPT pre-incubated with Naja kaouthia venom at concentrations as low as 0.625 mg/ml showed 100% survival after a 24-h observation period. (2) In the proteomics study, mice injected with MPT pre-incubated with the Naja kaouthia venom showed down-regulation of five serum proteins. (3) In the protein-dye-binding study, the percentage of Bradford dye-protein binding showed a reduction relative to the decrease in MPT concentration used to incubate with the venom. In vivo: the results from the animal studies showed that MPT had no in vivo protection against the Naja kaouthia venom (0.875 mg/kg) in four different rescue modes and in an oral pre-treatment experiment. The study indicated the promising ability of MPT to neutralize the Naja kaouthia venom in in vitro experiments but fell short in its in vivo potential. As such, the use of Mimosa pudica (Mimosaceae) as therapeutics for snake bites is questionable as all the possible in vivo rescue studies and pre-treatment of the active constituents showed no protection against the affected mice. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

  12. Antioxidant activities of isolated compounds from stems of Mimosa invisa Mart. ex Colla

    Energy Technology Data Exchange (ETDEWEB)

    Aguiar, Rosane M. [Departamento de Quimica e Exatas, Universidade Estadual do Sudoeste da Bahia, Jequie, BA (Brazil); Alves, Clayton Q.; David, Jorge M.; Rezende, Larissa C. de; Lima, Luciano S., E-mail: jmdavid@ufba.br [Instituto de Quimica, Universidade Federal da Bahia, Salvador, BA (Brazil); David, Juceni P. [Faculdade de Farmacia, Universidade Federal da Bahia, Salvador, BA (Brazil); Queiroz, Luciano P. de [Departamento de Ciencias Biologicas, Universidade Estadual de Feira de Santana, BA (Brazil)

    2012-07-01

    This work describes the phytochemical study of stems of Mimosa invisa (Mimosaceae) and the evaluation of the antioxidant potential of isolated compounds. Chromatographic techniques were employed to isolate salicifoliol, pinoresinol, quercetin, quercetin-3-Orhamnopyranosyl, quercetin-3-O-arabinofuranosyl lupeol, -amyrin, sitosterol, p-hydroxy coumaric acid, 4-hydroxy-3-methoxy benzaldehyde (vanillin), 4-hydroxy-3,5-dimethoxy benzaldehyde, 4-hydroxy-3-methoxy benzoic acid and 4',6,7- trimethoxy flavonol. The latter had been previously described but the spectrometric data shown indicated the structure required review. The antioxidant activity of the compounds was evaluated by the DPPH test and capability of NBT reduction by superoxide radicals. Quercetin glycosides showed lower antioxidant potential than quercetin and, salicifoliol was found to be more active than pinoresinol. (author)

  13. Antioxidant activities of isolated compounds from stems of Mimosa invisa Mart. ex Colla

    Directory of Open Access Journals (Sweden)

    Rosane M. Aguiar

    2012-01-01

    Full Text Available This work describes the phytochemical study of stems of Mimosa invisa (Mimosaceae and the evaluation of the antioxidant potential of isolated compounds. Cromatografic techniques were employed to isolate salicifoliol, pinoresinol, quercetin, quercetin-3-O-rhamnopyranosyl, quercetin-3-O-arabinofuranosyl lupeol, β-amyrin, sitosterol, p-hydroxy coumaric acid, 4-hydroxy-3-methoxy benzaldehyde (vanillin, 4-hydroxy-3,5-dimethoxy benzaldehyde, 4-hydroxy-3-methoxy benzoic acid and 4',6,7- trimethoxy flavonol. The latter had been previously described but the spectrometric data shown indicated the structure required review. The antioxidant activity of the compounds was evaluated by the DPPH test and capability of NBT reduction by superoxide radicals. Quercetin glycosides showed lower antioxidant potential than quercetin and, salicifoliol was found to be more active than pinoresinol.

  14. Dyeing of white and indigo dyed cotton fabrics with Mimosa tenuiflora extract

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    Gökhan Erkan

    2014-04-01

    Full Text Available Mimosa tenuiflora extract has been used in food industry as an additive and in textile and leather industry as a colorant. Two types of fabrics, ready to be dyed white and indigo dyed fabrics, were dyed with M. tenuiflora extract. The fabrics were mordanted after dyeing with six different metal salts. Colorimetric evaluations of fabrics were carried out by spectrophotometer. Colour fastness to washing, rubbing and light were performed. Colour strength of fabrics was calculated from Kubelka–Munk formula. Highest vividness (C∗ values were obtained by Ni mordant. Moderate fastness values were observed. However poor wet rubbing fastness values were observed in the case of indigo dyed fabrics due to lack of good wet rubbing fastness of indigo itself.

  15. Efficacy of tannins from Mimosa pudica and tannic acid in neutralizing cobra (Naja kaouthia venom

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    FY Sia

    2011-01-01

    Full Text Available In the present study, the effectiveness of Mimosa pudica tannins (MPT in neutralizing the lethality of Naja kaouthia venom was compared with commercially derived tannins. Preincubation of MPT with N. kaouthia venom maintained 100% survival of mice after 24 hours. The mouse group in which there was no preincubation, no protection against the effects of the venom was observed. M. pudica tannin was found to be more effective in neutralizing the lethality of N. kaouthia venom when compared to commercial tannic acid. Two protein spots were missing in the two-dimensional gel electrophoresis (2-DE of the MPT treated mouse indicating the down-regulation of venom proteins. The results from this study indicated that tannins obtained from M. pudica are better than tannic acid in neutralizing the lethality of N. kaouthia venom in vitro. However, further investigations are required to establish that M. pudica has potential for treating N. kaouthia snakebites.

  16. Genome sequence of Burkholderia mimosarum strain LMG 23256(T), a Mimosa pigra microsymbiont from Anso, Taiwan.

    Science.gov (United States)

    Willems, Anne; Tian, Rui; Bräu, Lambert; Goodwin, Lynne; Han, James; Liolios, Konstantinos; Huntemann, Marcel; Pati, Amrita; Woyke, Tanja; Mavrommatis, Konstantinos; Markowitz, Victor; Ivanova, Natalia; Kyrpides, Nikos; Reeve, Wayne

    2014-06-15

    Burkholderia mimosarum strain LMG 23256(T) is an aerobic, motile, Gram-negative, non-spore-forming rod that can exist as a soil saprophyte or as a legume microsymbiont of Mimosa pigra (giant sensitive plant). LMG 23256(T) was isolated from a nodule recovered from the roots of the M. pigra growing in Anso, Taiwan. LMG 23256(T) is highly effective at fixing nitrogen with M. pigra. Here we describe the features of B. mimosarum strain LMG 23256(T), together with genome sequence information and its annotation. The 8,410,967 bp high-quality-draft genome is arranged into 268 scaffolds of 270 contigs containing 7,800 protein-coding genes and 85 RNA-only encoding genes, and is one of 100 rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project.

  17. Genome sequence of Burkholderia mimosarum strain LMG 23256T, a Mimosa pigra microsymbiont from Anso, Taiwan

    Science.gov (United States)

    Willems, Anne; Tian, Rui; Bräu, Lambert; Goodwin, Lynne; Han, James; Liolios, Konstantinos; Huntemann, Marcel; Pati, Amrita; Woyke, Tanja; Mavrommatis, Konstantinos; Markowitz, Victor; Ivanova, Natalia; Kyrpides, Nikos; Reeve, Wayne

    2013-01-01

    Burkholderia mimosarum strain LMG 23256T is an aerobic, motile, Gram-negative, non-spore-forming rod that can exist as a soil saprophyte or as a legume microsymbiont of Mimosa pigra (giant sensitive plant). LMG 23256T was isolated from a nodule recovered from the roots of the M. pigra growing in Anso, Taiwan. LMG 23256T is highly effective at fixing nitrogen with M. pigra. Here we describe the features of B. mimosarum strain LMG 23256T, together with genome sequence information and its annotation. The 8,410,967 bp high-quality-draft genome is arranged into 268 scaffolds of 270 contigs containing 7,800 protein-coding genes and 85 RNA-only encoding genes, and is one of 100 rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project. PMID:25197434

  18. ANATOMIA DA MADEIRA E CASCA DO MARICÁ, Mimosa bimucronata (DC. O. Kuntze

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    José Newton Cardoso Marchiori

    1993-12-01

    Full Text Available São descritos os caracteres gerais, macroscópicos e microscópicos da madeira de Mimosa bimucronata (DC. O. Kutze, bem como a estrutura de sua casca. A anatomia da madeira é comparada com referências da literatura para espécies afins. Os caracteres observados incluem placa de perfuração simples em vasos, pontuados alternos, pontuações ornamentadas, parênquima paratraqueal, fibras libriformes, raios homogêneos de células procumbentes e ausência de estratificação. Na estrutura da casca destacam-se o líber duro em faixas tangenciais descontínuas, envolvidas por células parenquimáticas geralmente cristalíferas, o curso irregular dos raios na casca interna e a formação de cunhas de parênquima na casca mediana.

  19. Flavonoides e outros compostos isolados de Mimosa artemisiana Heringer e Paula

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    Ildomar Alves do Nascimento

    2012-01-01

    Full Text Available This the first phytochemical investigation of Mimosa artemisiana (Leguminosae-Mimosoideae describing the isolation and identification of quercitrin, myricitrin, 3,5,4´-trihydroxi-6,7-dimethoxyflavone (6,7-dimethylkaepferol, flavolignans, 3-O-β-D-glucopyranosil sitosterol, lupeol, sitostenone, stigmastenone, campestenone, sitosterol, stigmasterol, campesterol, methyl indole-3-carboxilate and indole-3-carboxaldehyde in the extracts from the leaves and wood of this plant. This is the first registry of 6,7-dimethoxy,4'-hydroxy-flavona and the flavonolignans in this genera. The isolation of all metabolites was made by chromatographic methods and the structures were established on the basis of IR, MS, ¹H and 13C NMR spectra analysis, comparison with literature data and GC-MS of mixtures analysis.

  1. A new fatty aldol ester from the aerial part of Mimosa invisa (Mimosaceae).

    Science.gov (United States)

    Nana, Frederic; Sandjo, Louis Pergaud; Keumedjio, Felix; Kuete, Victor; Ngadjui, Bonaventure Tchaleu

    2012-01-01

    A new aldol ester named 17-O-triacontanoylheptadecanal (1) was isolated from the aerial part of Mimosa invisa (Mimosaceae) together with eight known compounds identified as β-sitosterol (2), α-amyrine (3), lupeol (4), 4'-O-methylepinumisoflavone (5), alpinumisoflavone (6), betulinic acid (7), 3-O-β-D-glucopyranoside of sitosterol (8) and epirobinetinidol (9). The structures of compounds were determined on the basis of NMR and mass spectrometry data as well as by comparing the data reported in the literatures. The antimicrobial activities of the crude extract and compounds 1 and 9 were investigated against seven microbial species. The natural products showed moderate activities compared to that of the crude extract.

  2. Change in the actin cytoskeleton during seismonastic movement of Mimosa pudica.

    Science.gov (United States)

    Kanzawa, Nobuyuki; Hoshino, Yoshinori; Chiba, Makiko; Hoshino, Daisuke; Kobayashi, Hidetaka; Kamasawa, Naomi; Kishi, Yoshiro; Osumi, Masako; Sameshima, Masazumi; Tsuchiya, Takahide

    2006-04-01

    The seismonastic movement of Mimosa pudica is triggered by a sudden loss of turgor pressure. In the present study, we compared the cell cytoskeleton by immunofluorescence analysis before and after movement, and the effects of actin- and microtubule-targeted drugs were examined by injecting them into the cut pulvinus. We found that fragmentation of actin filaments and microtubules occurs during bending, although the actin cytoskeleton, but not the microtubules, was involved in regulation of the movement. Transmission electron microscopy revealed that actin cables became loose after the bending. We injected phosphatase inhibitors into the severed pulvinus to examine the effects of such inhibitors on the actin cytoskeleton. We found that changes in actin isoforms, fragmentation of actin filaments and the bending movement were all inhibited after injection of a tyrosine phosphatase inhibitor. We thus propose that the phosphorylation status of actin at tyrosine residues affects the dynamic reorganization of actin filaments and causes seismonastic movement.

  3. Antitoxin activity of Mimosa pudica root extracts against Naja naja and Bangarus caerulus venoms

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    Subramani Meenatchisundaram, Selvin Priyagrace, Ramasamy Vijayaraghavan, Ambikapathi Velmurugan, Govindarajan Parameswari, Antonysamy Michael

    2009-12-01

    Full Text Available Aqueous extract of dried roots of Mimosa pudica was tested for inhibitory activity on lethality, phospholipase activity, edema forming activity, fibrinolytic activity and hemorrhagic activity of Naja naja and Bangarus caerulus venoms. The aqueous extract displayed a significant inhibitory effect on the lethality, phospholipase activity, edema forming activity, fibrinolytic activity and hemorrhagic activity. About 0.14 mg and 0.16 mg of M. pudica extracts were able to completely neutralize the lethal activity of 2LD50 of Naja naja and Bangarus caerulus venoms respectively. The present finding suggests that aqueous extract of M. pudica root possesses compounds, which inhibit the activity of Naja naja and Bangarus caerulus venoms.

  4. Effects of Mimosa pudica L. leaves extract on anxiety, depression and memory

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    Ganesh Patro

    2016-11-01

    Full Text Available Objective: The present study was carried out to investigate the neuropharmacological activities of ethyl acetate extract of Mimosa pudica (EAMP leaves on anxiety, depression and memory in a mouse model. Materials and Methods: Anti-anxiety potential of EAMP was evaluated by elevated plus maze (EPM, light-dark box (LDB and social interaction (SI tests in mice.Anti-depressant potential of EAMP was evaluated by forced swimming (FST, tail suspension (TST, and open field tests (OFT. The behavioral findings were further corroborated with estimation of neurotransmitters and their metabolites from mouse brain homogenate. Effect on learning and memory was evaluated by EPM, passive avoidance (PA tests. Further, it was confirmed with assessment of acetylcholinesterase and caspase-3 activity in brain homogenate. Results: EAMP showed significant anti-anxiety activity by increasing the time spent in open arm of EPM, light box of LDB. Social interaction time was increased significantly (p

  5. Antitoxin activity of Mimosa pudica root extracts against Naja naja and Bangarus caerulus venoms

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    Subramani Meenatchisundaram

    2009-06-01

    Full Text Available Aqueous extract of dried roots of Mimosa pudica was tested for inhibitory activity on lethality, phospholipase activity, edema forming activity, fibrinolytic activity and hemorrhagic activity of Naja naja and Bangarus caerulus venoms. The aqueous extract displayed a significant inhibitory effect on the lethality, phospholipase activity, edema forming activity, fibrinolytic activity and hemorrhagic activity. About 0.14 mg and 0.16 mg of M. pudica extracts were able to completely neutralize the lethal activity of 2LD50 of Naja naja and Bangarus caerulus venoms respectively. The present finding suggests that aqueous extract of M. pudica root possesses compounds, which inhibit the activity of Naja naja and Bangarus caerulus venoms.

  6. Evaluation of antifungal activity of standardized extract of Salvia rhytidea Benth. (Lamiaceae) against various Candida isolates.

    Science.gov (United States)

    Salari, S; Bakhshi, T; Sharififar, F; Naseri, A; Ghasemi Nejad Almani, P

    2016-12-01

    Salvia species have long been described in traditional medicine for various indications. Owing to the widespread use of this genus by ethnic populations, especially for various infections ranging from skin disease to gastrointestinal disorders, we were encouraged to determine whether Salvia rhytidea could be effective against fungal infections. Given the increased incidence of candidiasis in the past decade, limits on the use of antifungal drugs, emergence of azole-resistant Candida species and increased incidence of treatment failures, it is necessary to identify a novel agent with antifungal properties. Aim of the study was to evaluate the antifungal properties of S. rhytidea against various Candida isolates. In this study, at first rosmarinic acid content of plant extract was determined. A total of 96 Candida isolates were tested, including the following species: Candida albicans (n=42), Candida glabrata (n=16), Candida tropicalis (n=11), Candida krusei (n=9), Candida parapsilosis (n=9), Candida lusitaniae (n=7) and Candida guilliermondii (n=2). The in vitro antifungal activity of methanolic extracts of S. rhytidea Benth. was evaluated against Candida isolates and compared with that of the standard antifungal drug nystatin by using a broth microdilution method, according to CLSI. Phytochemical screening results showed that the methanolic extract of S. rhytidea Benth. was rich in flavonoids and tannins. The minimal inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values of S. rhytidea Benth. ranged from 3.125 to>100μg/ml and 6.25 to>100μg/ml respectively. The growth inhibition value displayed that C. tropicalis, C. krusei and C. albicans isolates were most susceptible to S. rhytidea. Findings show that S. rhytidea possesses an antifungal effect against Candida isolates. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  7. ABEJAS VISITANTES DE Mimosa pigra L. (MIMOSACEAE: COMPORTAMIENTO DE PECOREO Y CARGAS POLÍNICAS

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    CLARA ISABEL AGUILAR SIERRA

    2009-01-01

    Full Text Available Se estudiaron las cargas polínicas de 13 taxones de abejas capturadas visitando a Mimosa pigra en la zona de influencia del Embalse Porce II (Antioquia, Colombia. De los 21 tipos polínicos encontrados, M. pigra presenta el mayor porcentaje acumulado de colecta; Mimosa pudica, Piper aduncum, Solanum diversifolium, Warszewiczia coccinea y Psidium guajava, en su orden, se pueden considerar fuentes alternativas de polen, para varias de las espe- cies de abejas cuyas cargas polínicas fueron analizadas. Dentro de las especies de abejas capturadas visitando a M. pigra se pueden diferenciar varios grupos según el tipo y abun- dancia relativa de los tipos polínicos encontrados en sus cargas. Uno de ellos, incluye a siete especies de abejas con más del 85% de granos de polen de M. pigra; otro, con cuatro especies de abejas que colectaron más del 94,5% del polen en M. pigra y M. pudica. Adi- cionalmente, se encontraron especies como Trigona dorsalis con cargas de M. pigra (59,4%, de S. diversifolium (37,8% y especies de abejas como Lasioglossum sp. 113, en cuyas cargas polínicas predominan los granos de polen de P. aduncum (61,8% y de W. coccinea (36,4%, en contraste con los de M. pigra (1,3%. En cuanto a la riqueza de tipos polínicos colectados por las abejas sobresalen Trigona muzoensis (12 tipos polí- nicos y T. dorsalis (10 tipos, lo cual refleja nichos tróficos más amplios para estas especies y deja duda sobre su constancia floral o hábitos de limpieza.

  8. Escarificación artificial y natural de la semilla de Lupinus bilineatus Benth

    OpenAIRE

    Martínez, J. M.; D. A. Rodríguez-Trejo; E. Guizar-Nolazco; R. Bonilla-Beas

    2008-01-01

    Las especies de Lupinus colonizan áreas incendiadas y fijan N. En otros países las especies de este género se emplean en plantaciones forestales y sistemas agroforestales. Sus semillas presentan latencia física. En el presente estudio se realizó el análisis de semillas y se evaluó la germinación de Lupinus bilineatus Benth. considerando los siguientes factores y niveles: escarificación (natural: fuego, humo; artificial: inmersión en ácido sulfúrico durante 10, 20, 30 y 40 min y control), régi...

  9. Two New Koumine-Type Indole Alkaloids from Gelsemium elegans Benth.

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    Huanhuan Gao

    2013-01-01

    Full Text Available Two new indole alkaloids, 21-oxokoumine (1 and furanokoumine (2, were isolated from the roots of Gelsemium elegans Benth together with three known compounds. The structures of the two novel compounds were elucidated by spectroscopic methods, including NMR, HR-ESI-MS, UV, IR, CD and molecular modeling. Compound 1 is the first instance of a koumine-type alkaloid with a carbonyl at the C-21 position, while compound 2 possesses a tetrahydrofuran ring located on C-20 and C-21.

  10. [First record of Platycorypha nigrivirga Burckhardt (Hemiptera: Psylloidea), in Tipuana tipu (Benth.), from Brazil].

    Science.gov (United States)

    Santana, Dalva L Q; Burckhardt, Daniel; Aguiar, António M F

    2006-01-01

    The occurrence of Platycorypha nigrivirga Burckhardt (Hemiptera: Psyllidae, Acizzinae), is reported for the first time, in Brazil. The psyllid was observed causing injuries on leaves and shoots of Tipuana tipu (Benth.), in urban areas of Paraná State. P. nigrivirga are tiny sap-sucking insects that feed on phloem and cause curling, stains and fall of leaves. The excrements of these insects accumulate on the leaves propitiating fungus growth on the leaves, or they fall in great amount on the cars dirtying them as if they were sparkling of white and sticky ink.

  11. Evaluation of the immunotoxicological effects of Dimorphandra mollis Benth., Fabaceae, in rats

    OpenAIRE

    2010-01-01

    Dimorphandra mollis Benth., Fabaceae, also known as "faveira" or "fava-d'anta", is a plant common to the central woodsy meadow region of Brazil. It is well known for its antioxidant, antiplatelet and, principally, vasoprotective properties. Its principal component is rutin. The objective of this study is the evaluation of the safety of the use of the dried D. mollis extract in rodents. The rutin content of the standardized extract was 76.0±3%. With respect to the biochemical and hematological...

  12. Antioxidant activity of A New Flavone Glycoside from the seeds of Albizzia Odoratissima Benth.

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    Prakrati Yadav

    2013-11-01

    Full Text Available A new compound (A  3,5,7,3¢-tetrahydroxy-4¢-methoxyflavone-3-O-α-L-rhamnopyranosyl-7-O-β-D-xylopyranosyl (1®2O-β-D-glucopyranoside alongwith with  two known compounds Luteolin (B and  Acacetin  (C were isolated from methanolic extracts of the defatted seeds of Albizzia Odoratissima Benth. The structure of a new compound was elucidated on the basis of extensive spectroscopic analysis, colour reactions and chemical degradations. Compound A exhibited higher radical scavenging activity in the 1,1-diphenyl-2-pycrylhydrazyl (DPPH assay system.  

  13. A new triterpenoid saponin from the root of Croton lachnocarpus Benth.

    Science.gov (United States)

    Pan, Zheng-Hong; Ning, De-Sheng; Liu, Jin-Lei; Pan, Bo; Li, Dian-Peng

    2014-01-01

    A new triterpenoid saponin, 3-O-β-D-xylopyranosyl spathodic acid (1), was isolated from the EtOH extract of the root of Croton lachnocarpus Benth., together with four known compounds. These compounds were characterised on the basis of their spectral data and compatible with values in the literature. Compound 1 was the first triterpenoid glucoside isolated from the genus Croton. The known compound myriaboric acid (2) showed cytotoxic activity against human hepatocellular carcinoma SMMC-7721 cell line with an IC50 value of 42.2 μM.

  14. Chemical Composition and Antimicrobial Activity of Essential Oil of Lepechiniaradula Benth Epling

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    Vladimir Morocho

    2017-01-01

    Full Text Available The essential oil (EO was obtained by hydrodistillation from the aerial parts of Lepechiniaradula Benth Epling (Lamiaceae from Ecuador. Thirty-four compounds accounting to 93.4% of the total oil were identified. The main constituents of the essential oil were δ-3-carene (19.9%, β-pinene (17.0%, (E-β-caryophyllene (9.7% and (E-E-α-farnesene (9.4%. The essential oil of L.radula possessed strong antifungal activity against Trichophytonrubrum (ATCC® 28188 and Trichophytonmentagrophytes (ATCC® 28185.

  15. Estrutura anatômica da madeira e qualidade do carvão de Mimosa tenuiflora (Willd. Poir Anatonical structure and charcoal quality of Mimosa tenuiflora (Willd. Poir. Wood

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    Elisabeth de Oliveira

    2006-04-01

    Full Text Available Este trabalho objetivou determinar algumas características anatômicas e dimensões de fibras, elementos dos vasos, células do parênquima e dos raios da madeira da Mimosa tenuiflora (Willd. Poir. Determinaram-se, ainda, a fração parede das fibras e o porcentual das fibras, dos vasos, dos raios e das células parenquimatosas, bem como a densidade, rendimento e propriedades do carvão vegetal. Conclui-se que a madeira de Mimosa tenuiflora possui poros predominantemente solitários, geminados e múltiplos em agrupamento radial; poros distribuídos em porosidade difusa uniforme; parênquima axial paratraqueal vasicêntrico, vasicêntrico confluente, aliforme e aliforme confluente; raios multisseriados, bisseriados e, menos freqüentemente, unisseriados; e fibras de parede espessa e muito curtas. Obteve-se um rendimento de 39,68% em carvão vegetal, com teor de carbono fixo de 71,70%, densidade igual a 0,51g/cm³, carbono fixo de 71,79 e poder calórico de 6.866 cal/g.The objective of this work was to determine anatomical characteristics, and dimensions of fibers, vessels and parenchyma and ray cells of Mimosa tenuiflora (Willd. Poir wood. Fibers wall fraction, the percentage of fibers, vessels and ray and parenchyma cells, as well as density and charcoal production and properties were determined. The wood of Mimosa teniflora presents predominantly solitary, geminated and multiple porous in radial groups; porous distributed in uniform diffuse porosity; axial parenchyma paratracheal vasicentric, confluent vasicentric, aliform and confluent aliform; multiseriate, biseiat rays and, less frequently, uniseriate ones; very short fibers with thick walls. Charcoal yield was 39.68% with a 0.51g/cm³ density, 71.79% carbon content and 6886cal/g calorific value.

  16. Sobrevivência e crescimento de mudas de sabiá em podzólico vermelho-amarelo sob erosão simulada Survival and growth of mimosa caesalpiniaefolia seedlings on a red-yellow podzolic soil under simulated erosion

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    JOSÉ RONALDO COELHO SILVA

    2000-05-01

    Full Text Available O objetivo deste trabalho foi estudar mudanças nas propriedades de um Podzólico Vermelho-Amarelo relacionadas à remoção gradual de sua camada arável por meio de erosão simulada e seus efeitos na sobrevivência e crescimento de mudas de sabiá (Mimosa caesalpiniaefolia Benth. O experimento foi conduzido em casa de vegetação, em 1990, no Departamento de Ciência do Solo, UFC, em Fortaleza, CE, em camadas do solo de 0-15, 5-20, 10-25, 15-30, 20-35 e 25-40 cm, as quais foram removidas e acondicionadas em sacos de plástico, onde as sementes foram plantadas e as mudas desenvolveram-se por 85 dias. Maiores valores de porcentagem de argila, de água disponível, de teores de K e Ca e da CTC determinados nas camadas mais profundas não reduziram o impacto negativo da erosão na sobrevivência e crescimento das mudas, pois eles estavam associados a aumentos da saturação por Al, bem como a significativos decréscimos no pH, na matéria orgânica, na saturação por bases e nos teores de N, P e Mg no solo. À medida que a profundidade das camadas removidas aumentava, a porcentagem de sobrevivência, a altura, o peso seco das raízes e da parte aérea, bem como a biomassa seca total das plantas gradualmente decrescia; esses decréscimos foram de 16,8, 66,3, 67,1, 82,0 e 79,6%, respectivamente, na camada de 25-40 cm, em relação aos dados da camada 0-15 cm.The objective of this research was to determine changes in properties of a Red-Yellow Podzolic soil, related to the gradual removal of the plow layer through simulated erosion and its effects on survival and growth of Mimosa caesalpiniaefolia Benth seedlings. In 1990, the experiment was conducted in a greenhouse of the Universidade Federal do Ceará, in Fortaleza, CE, Brazil, in layers of 0-15, 5-20, 10-25, 15-30, 20-35 and 25-40 cm which were removed from a Red-Yellow Podzolic profile and contained in plastic bags where seeds were planted and seedlings were grown for 85 days. Higher values of

  17. Synthesis and Characterization of Mimosa Pudica Leaves Shaped α-Iron Oxide Nanostructures for Ethanol Chemical Sensor Applications.

    Science.gov (United States)

    Kim, S H; Ibrahim, Ahmed A; Kumar, R; Umar, Ahmad; Abaker, M; Hwang, S W; Baskoutas, S

    2016-03-01

    Herein, the synthesis of mimosa pudica leaves shaped a-iron oxide (α-Fe2O3) nanostructures is reported through simple and facile hydrothermal process. The prepared α-Fe2O3 nanostructures were characterized in terms of their morphological, structural, compositional and optical properties through a variety of characterization techniques such as FESEM, EDS, XRD, FTIR and Raman spectroscopy. The detailed characterizations revealed the well-crystallinity and dense growth of mimosa pudica leaf shaped α-Fe2O3 nanostructures. Further, the prepared nanomaterials were used as efficient electron mediator to fabricate sensitive ethanol chemical sensor. The fabricated sensor exhibited a high sensitivity of -30.37 μAmM(-1) cm(-2) and low detection limit of -0.62 μM. The observed linear dynamic range (LDR) was in the range from 10 μM-0.625 μM.

  18. Germinación y establecimiento de Mimosa aculeaticarpa var. biuncifera (Fabaceae-Mimosoideae Seed germination and seedlings establishment of Mimosa aculeaticarpa var. biuncifera (Fabaceae-Mimosoideae

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    Numa P. Pavón

    2011-06-01

    Full Text Available El arbusto deciduo espinoso Mimosa aculeaticarpa var. biuncifera que se distribuye en las zonas semiáridas de México tiene potencial para usarse en proyectos de restauración ecológica; sin embargo, no existe información básica que sustente esta afirmación. Evaluar las condiciones óptimas de germinación y establecimiento de la especie fue el objetivo de este estudio. En cuanto a la germinación, se experimentó con 3 factores (escarificación, luz y temperatura y adicionalmente se determinó la depredación por brúquidos. Para evaluar el establecimiento se midió el efecto de la luz y el nitrógeno en el suelo sobre la sobrevivencia, el crecimiento y la nodulación de las raíces de las plántulas. La escarificación y la temperatura fueron factores significativos en la germinación. Las semillas no fueron fotoblásticas y los parámetros germinativos indicaron que los mejores resultados de germinación se obtuvieron a 30º C. Las semillas dañadas por los brúquidos no germinaron, la infestación fue de 26.8 % y se definieron 4 especies de brúquidos. La mayor sobrevivencia de plántulas y el mayor crecimiento de las raíces se registraron en condiciones de alta luminosidad. La fertilización del suelo con nitrógeno tuvo efecto negativo significante sobre la sobrevivencia y crecimiento de las plántulas. Estos resultados apoyan la recomendación de usar a M. aculeaticarpa var. biuncifera en proyectos de restauración de zonas semiáridas en México.Mimosa aculeaticarpa var. biuncifera, spiny drought-deciduous shrub has the potential to be used in restoration projects in degraded semi-arid areas of México. However, basic information that supports this does not exist. The objective of the study was to evaluate the germination conditions and establishment of this species. Germination experiments were realized using 3 factors (scarification, light and temperature. Also, seeds predation for bruquids was registered. We evaluated the effect

  19. Vascular Reactivity Concerning Orthosiphon stamineus Benth-Mediated Antihypertensive in Aortic Rings of Spontaneously Hypertensive Rats

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    Nurul Maizan Manshor

    2013-01-01

    Full Text Available Orthosiphon stamineus Benth has been traditionally used to treat hypertension. The study aimed to investigate the vascular reactivity of water extract (WOS and water : methanolic (1 : 1 extract (WMOS of Orthosiphon stamineus Benth and AT1 receptors blocker in the mechanisms of antihypertensive mediated by α1-adrenergic receptor and EDNO and PGI2 releases in the SHR aortic rings. SHR (230–280 g were divided into four groups: control, WOS, WMOS, and losartan. After being fed orally for 14 days, the aorta was harvested and subjected to PE (10−9 to 10−5 M and ACh (10−9 to 10−5 M with and without L-NAME (100 µM and indomethacin (10 µM, respectively. WOS, WMOS, and losartan significantly reduced the contractile responses to PE intact suggesting the importance of endothelium in vasorelaxation. Losartan significantly enhanced the ACh-induced vasorelaxation. L-NAME significantly inhibited the ACh-induced relaxation in all groups. Indomethacin enhanced ACh-induced vasorelaxation in WMOS. Collectively, Orthosiphon stamineus leaves extract reduced vasoconstriction responses by the alteration of α1-adrenergic and AT1 receptors activities. The involvement of EDNO releases was clearly observed in this plant. In WOS, PGI2 releases might not participate in the ACh-induced vasorelaxation. However, in WMOS, enhancement of vasorelaxation possibly due to continuous release of PGI2.

  20. Morfologia e anatomia foliar de Bauhinia curvulha Benth. (Leguminosae-Caesalpinioideae Morphology and anatomy of Bauhinia curvula Benth. (Leguminosae-Caesalpinoideae

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    Maria Helena Rezende

    1994-07-01

    Full Text Available Foi realizado o estudo morfológico e anatômico da folha de Bauhinia cumula Benth., espécie de cerrado. As observações foram feitas em folhas adultas, tratadas segundo a metodologia usual utilizada em anatomia vegetal. Foi observado que a espécie apresenta as seguintes características: folhas bilobadas cuja incisão foliar chega próximo à região motora; um par de estipulas membranáceas; um par de nectários extraflorais, localizadaos na base do pulvino proximal; a venação possui padrão acródomo; o pecíolo, que possui dois pulvinos, um proximal e um distai, apresenta epiderme contendo grande quantidade de tricomas tecto res simples e glândulas; a lâmina foliar é anfiestomática com predomínio de estômatos dos tipos anomocítico e paracítico; a epiderme adaxial é monoestratificada, subpapilosa, desprovida de tricomas tectores simples e glândulas; a epiderme abaxial é subpapilosa, apresentando numerosos tricomas tectores simples e glândulas; e o mesofilo é formado predominantemente por parênquima paliçádico.A morphological and anatomical study of Bauhinia curvula Benth. leaf, a cerrado spe-cies, was perfomed. The observations, in grown up leaves, prepared according to the costumary methodology used in plant anatomy, showed the following characteristics of the species: bilobed leaves with foliar incision close to the motor region; one pair of membranaceous stipules; one pair of extrafloral nectaries located on the basis of the proximal pulvinus; acrodromous patterns of leaf venetion; the petiole, which has two pulvinus, one proximal and one distal, has an epidermis containing large quantities of simple tector trichomes and glands; the leaf blade, amphiestomatic, with predominance of anomocytic and paracytic stomata; the adaxial epidermis uniserial, sub papillose, without tector trichomes and glands; the abaxial epiderms, sub-papillose, presents a high number of simple trichomes and glands; and the mesophyll with a basic

  1. Histochemical and ultrastructural study of Caesalpinia peltophoroides Benth. (Leguminosae-Caesalpinoideae seeds Estudo histoquímico e ultraestrutural de sementes de Caesalpinia peltophoroides Benth. (Leguminosae-Caesalpinoideae

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    Viviana Borges Corte

    2009-10-01

    Full Text Available The objective of this work was to correlate data on light microscopy observations through histochemical analysis and polarized light techniques and investigations in transmission electron microscopy (TEM to characterize the reserve materials in C. peltophoroides Benth. (Leguminosae-Caesalpinoideae cotyledons, popularly known as "sibipiruna", a tropical tree species with wide distribution in Brazil. The cotyledon mesophyll, especially in the abaxial face, is rich in unsaturated neutral lipids contained in numerous lipid bodies dispersed in the cytoplasm. Proteins, more concentrated in the adaxial face of the cotyledons, occur in all the mesophyll and are stored in protein bodies containing globoids, with variable number and size, responsible for accumulation of mineral reserves. Calcium oxalate druses have distribution restricted to the cotyledons adaxial face and are associated with protein bodies. Starch, also distributed all over the cotyledon mesophyll, occurs in small amounts in plastids with developed lamellar system. Secretory cavities rich in phenolic compounds occur among procambial strands.Este trabalho procurou correlacionar dados de observações em microscopia de luz através de técnicas histoquímicas e de luz polarizada e investigações em microscopia eletrônica de transmissão para caracterizar os materiais de reserva em cotilédones de C. peltophoroides Benth. (Leguminosae-Caesalpinoideae, conhecida popularmente como sibipiruna, ou falso pau-brasil, uma espécie tropical com ampla distribuição no Brasil. O mesofilo cotiledonar, especialmente na face abaxial, apresenta-se rico em lipídios neutros, insaturados, contidos em numerosos corpos lipídicos dispersos no citoplasma. As proteínas, mais concentradas na face adaxial dos cotilédones, ocorrem em todo o mesofilo e são armazenadas em corpos proteicos contendo globoides, com número e tamanho variáveis, responsáveis pelo acúmulo de reservas minerais. Drusas de oxalato

  2. Dormancy and Germination of Castilla Blackberry Seeds (Rubus glaucus Benth / Latencia y Germinación de Semillas de Mora de Castilla (Rubus glaucus Benth

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    Díaz Diez Cipriano Arturo

    2013-08-01

    Full Text Available We categorized the dormancy and germination ofblackberry (Rubus glaucus Benth seeds from the GermplasmBank System for Food and Agriculture of the Colombian Nation. A tetrazolium test showed normal seedling production viability, but seed coat impermeability prevented imbibition, which is considered an expression of exogenous dormancy; however, this was released by immersing the seeds in 5.25% sodium hypochlorite for 16 to 21 hours. The treatment was applied to 10 accessions of Castilla blackberry, harvested during the dry and rainy seasons. The seeds were germinated under light and dark conditions. The highest germination counts were obtained with the dry-season-collected seeds incubated in darkness and with the rainy-season seeds incubated under light conditions. Differential germination responses were also independently determined by genotype, incubation conditions (light or darkness and collecting season. / Se categorizaron la latencia y la germinación ensemillas de mora de Castilla (Rubus glaucus Benth, provenientes del Sistema de Bancos de Germoplasma para la Alimentación y la Agricultura de la Nación Colombiana. La prueba del tetrazolio demostró que las semillas tenían la capacidad para dar origen a una plántula normal; sin embargo, al contacto con el agua la imbibición fue restringida por la impermeabilidad de la testa, lo que se considera como una expresión de latencia exógena. Ésta pudo ser removida con la inmersión de las semillas durante 16 a 21 horas en hipoclorito de sodio al 5,25%. El tratamiento fue aplicado a 10 accesiones de mora de Castila cosechadas en épocas seca y lluviosa; las semillas se germinaron bajo condiciones deluz y oscuridad, con una mayor germinación al incubar lasprovenientes de tiempo seco en condiciones de oscuridad y en aquellas de época lluviosa al ser germinadas con luz. También al aplicar el procedimiento de remoción de latencia exógena se observaron respuestas germinativas diferenciales

  3. Estudio comparativo de taninos de tara, mimosa y pino como recurtientes

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    Ricardo Hourdebaigt

    2011-05-01

    Full Text Available Los taninos vegetales son productos naturales que tienen la capacidad de formar complejos con proteínas. Dentro de este contexto, son los productos naturales más importantes usados industrialmente en los procesos que transforman las pieles en cueros. En este proyecto caracterizaremos los taninos utilizados (evaluación de su poder curtiente  y  partiendo de una formulación base en función del artículo final elegido (capellada de calzado, se realiza el curtido e iremos variando el curtiente vegetal y  evaluando  su comportamiento con distintos ensayos.Los taninos evaluados son mimosa, pino radiata y polvo de tara. También presentamos una caracterización de los cueros, mostrando los ensayos a realizar a los diferentes tipos de cuero en función de su uso, las normas que aplican para los análisis y especificaciones que nos orienten para los mismos. Estas especificaciones indican las propiedades que el cuero debe cumplir para que el mismo tenga un  comportamiento adecuado durante su fabricación y uso.AbstractThe vegetable tannins are natural products that are able to form complex with proteins. Within this context, are the most important natural products used in industrial processes that transform raw hides into leathers. In this project we wil characterize the tannins used (evaluation of their power tanning, starting with a basic formulation depending on the final article chosen (upper shoes, the process of tanning is carried out varying the vegetable tannin and evaluating its behaviour with different essays. The evaluated tannins are: mimosa, radiata pine and tare powder. We also present a leather characterization, showing the essays to carry out, the different types of leather depending on its use, the standars used for the analysis and specifications that guide us to them. These specifications indicate the properties that the leather must have to have appropiate behaviour during their manufacture and use.             

  4. Complete Genome sequence of Burkholderia phymatum STM815T, a broad host range and efficient nitrogen-fixing symbiont of Mimosa species

    Science.gov (United States)

    Moulin, Lionel; Klonowska, Agnieszka; Caroline, Bournaud; Booth, Kristina; Vriezen, Jan A.C.; Melkonian, Rémy; James, Euan K.; Young, J. Peter W.; Bena, Gilles; Hauser, Loren; Land, Miriam; Kyrpides, Nikos; Bruce, David; Chain, Patrick; Copeland, Alex; Pitluck, Sam; Woyke, Tanja; Lizotte-Waniewski, Michelle; Bristow, Jim; Riley, Margaret

    2014-01-01

    Burkholderia phymatum is a soil bacterium able to develop a nitrogen-fixing symbiosis with species of the legume genus Mimosa, and is frequently found associated specifically with Mimosa pudica. The type strain of the species, STM 815T, was isolated from a root nodule in French Guiana in 2000. The strain is an aerobic, motile, non-spore forming, Gram-negative rod, and is a highly competitive strain for nodulation compared to other Mimosa symbionts, as it also nodulates a broad range of other legume genera and species. The 8,676,562 bp genome is composed of two chromosomes (3,479,187 and 2,697,374 bp), a megaplasmid (1,904,893 bp) and a plasmid hosting the symbiotic functions (595,108 bp). PMID:25197461

  5. Complete Genome sequence of Burkholderia phymatum STM815, a broad host range and efficient nitrogen-fixing symbiont of Mimosa species

    Energy Technology Data Exchange (ETDEWEB)

    Moulin, Lionel [UMR, France; Klonowska, Agnieszka [UMR, France; Caroline, Bournaud [UMR, France; Booth, Kristina [University of Massachusetts; Vriezen, Jan A.C. [University of Massachusetts; Melkonian, Remy [UMR, France; James, Euan [James Hutton Institute, Dundee, United Kingdom; Young, Peter W. [University of York, United Kingdom; Bena, Gilles [UMR, France; Hauser, Loren John [ORNL; Land, Miriam L [ORNL; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute; Bruce, David [Los Alamos National Laboratory (LANL); Chain, Patrick S. G. [Lawrence Livermore National Laboratory (LLNL); Copeland, A [U.S. Department of Energy, Joint Genome Institute; Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Lizotte-Waniewski, Michelle [University of Massachusetts; Bristow, James [U.S. Department of Energy, Joint Genome Institute; Riley, Monica [Woods Hole Oceanographic Institution (WHOI), Woods Hole

    2014-01-01

    Burkholderia phymatum is a soil bacterium able to develop a nitrogen-fixing symbiosis with species of the legume genus Mimosa, and is frequently found associated specifically with Mimosa pudica. The type strain of the species, STM 815T, was isolated from a root nodule in French Guiana in 2000. The strain is an aerobic, motile, non-spore forming, Gram-negative rod, and is a highly competitive strain for nodulation compared to other Mimosa symbionts, as it also nodulates a broad range of other legume genera and species. The 8,676,562 bp genome is composed of two chromosomes (3,479,187 and 2,697,374 bp), a megaplasmid (1,904,893 bp) and a plasmid hosting the symbiotic functions (595,108 bp).

  6. Complete Genome sequence of Burkholderia phymatum STM815(T), a broad host range and efficient nitrogen-fixing symbiont of Mimosa species.

    Science.gov (United States)

    Moulin, Lionel; Klonowska, Agnieszka; Caroline, Bournaud; Booth, Kristina; Vriezen, Jan A C; Melkonian, Rémy; James, Euan K; Young, J Peter W; Bena, Gilles; Hauser, Loren; Land, Miriam; Kyrpides, Nikos; Bruce, David; Chain, Patrick; Copeland, Alex; Pitluck, Sam; Woyke, Tanja; Lizotte-Waniewski, Michelle; Bristow, Jim; Riley, Margaret

    2014-06-15

    Burkholderia phymatum is a soil bacterium able to develop a nitrogen-fixing symbiosis with species of the legume genus Mimosa, and is frequently found associated specifically with Mimosa pudica. The type strain of the species, STM 815(T), was isolated from a root nodule in French Guiana in 2000. The strain is an aerobic, motile, non-spore forming, Gram-negative rod, and is a highly competitive strain for nodulation compared to other Mimosa symbionts, as it also nodulates a broad range of other legume genera and species. The 8,676,562 bp genome is composed of two chromosomes (3,479,187 and 2,697,374 bp), a megaplasmid (1,904,893 bp) and a plasmid hosting the symbiotic functions (595,108 bp).

  7. Mimosa tenuiflora as a cause of malformations in ruminants in the northeastern Brazilian semiarid rangelands.

    Science.gov (United States)

    Pimentel, L A; Correa, F Riet; Gardner, D; Panter, K E; Dantas, A F M; Medeiros, R M T; Mota, R A; Araújo, J A S

    2007-11-01

    Craniofacial anomalies, eye malformations, and permanent flexures of the forelimbs are common malformations seen in ruminants grazing semiarid rangelands of Northeastern Brazil. To investigate the cause of these malformations, we fed 2 suspected plants, Mimosa tenuiflora or Prosopis juliflora, to groups of 4 pregnant goats each. Fresh green M. tenuiflora was collected daily and fed ad libitum to 4 goats in group 1 throughout pregnancy. This treatment group also received a supplemental feed concentrate equivalent to 1% body weight. Four goats in group 2 received a ration with 70% of P. juliflora pods and 30% hay throughout pregnancy. Four control goats were fed supplemental feed concentrate (1% body weight) and hay ad libitum throughout pregnancy. Goats treated with P. juliflora pods and the control goats delivered 9 normal kids. The four goats that were fed M. tenuiflora during pregnancy delivered 4 kids, 3 of which had abnormalities similar to those observed in field cases, including cleft lip, unilateral corneal opacity, ocular bilateral dermoids, buphthalmos with a cloudy brownish appearance of the anterior chamber due to an iridal cyst, and segmental stenosis of the colon. Malformations induced experimentally by M. tenuiflora were similar to those observed in field cases, suggesting that M. tenuiflora is a cause of the field cases observed in the Brazilian semiarid rangelands.

  8. Anatomia da madeira e casca do maricá, Mimosa bimucronata (DC. O. Kuntze.

    Directory of Open Access Journals (Sweden)

    José Newton Cardoso Marchiori

    2009-09-01

    Full Text Available Normal 0 21 false false false MicrosoftInternetExplorer4 São descritos os caracteres gerais, macroscópicos e microscópicos da madeira de Mimosa bimucronata (DC. O. Kutze, bem como a estrutura de sua casca. A anatomia da madeira é comparada com referências da literatura para espécies afins. Os caracteres observados incluem placa de perfuração simples em vasos, pontuados alternos, pontuações ornamentadas, parênquima paratraqueal, fibras libriformes, raios homogêneos de células procumbentes e ausência de estratificação. Na estrutura da casca destacam-se o líber duro em faixas tangenciais descontínuas, envolvidas por células parenquimáticas geralmente cristalíferas, o curso irregular dos raios na casca interna e a formação de cunhas de parênquima na casca mediana.

  9. CLIMATE-TREE GROWTH RELATIONSHIPS OF Mimosa tenuiflora IN SEASONALLY DRY TROPICAL FOREST, BRAZIL

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    Patrícia Póvoa Mattos

    2015-03-01

    Full Text Available Mimosa tenuiflora is a native pioneer tree from the Caatinga used commercially as firewood due to its high calorific value. It is deciduous, its trunk does not reach large diameters and it has good regrowth capacity. This study intended to determine the annual increment in diameter of M. tenuiflora and its correlation with rainfall, as basis for fuel wood management. Disks from the stem base of M. tenuiflora trees were collected in 2008 in Sertânia and Serra Talhada, Pernambuco State, from regrowth of trees coppiced in 2003 and in Limoeiro do Norte, Ceará State, from a plantation established in 2002. The trees have well-defined annual growth rings, highly correlated with annual precipitation and are well-suited for dendrochronological investigations. Forest managers must consider the influence of previous drier years in the wood production when predicting fuel wood harvesting. The high growth correlation with the previous year’s rainfall in regions where the rains start after photoperiodic stimulation indicate the necessity of understanding the growth dynamics of the species under dry forest conditions through additional ecophysiology studies.

  10. Arbuscular mycorrhizal fungi in Mimosa tenuiflora (Willd.) Poir from Brazilian semi-arid.

    Science.gov (United States)

    de Souza, Tancredo Augusto Feitosa; Rodriguez-Echeverría, Susana; de Andrade, Leonaldo Alves; Freitas, Helena

    2016-01-01

    Many plant species from Brazilian semi-arid present arbuscular mycorrhizal fungi (AMF) in their rhizosphere. These microorganisms play a key role in the establishment, growth, survival of plants and protection against drought, pathogenic fungi and nematodes. This study presents a quantitative analysis of the AMF species associated with Mimosa tenuiflora, an important native plant of the Caatinga flora. AMF diversity, spore abundance and root colonization were estimated in seven sampling locations in the Ceará and Paraíba States, during September of 2012. There were significant differences in soil properties, spore abundance, percentage of root colonization, and AMF diversity among sites. Altogether, 18 AMF species were identified, and spores of the genera Acaulospora, Claroideoglomus, Dentiscutata, Entrophospora, Funneliformis, Gigaspora, Glomus, Racocetra, Rhizoglomus and Scutellospora were observed. AMF species diversity and their spore abundance found in M. tenuiflora rhizosphere shown that this native plant species is an important host plant to AMF communities from Brazilian semi-arid region. We concluded that: (a) during the dry period and in semi-arid conditions, there is a high spore production in M. tenuiflora root zone; and (b) soil properties, as soil pH and available phosphorous, affect AMF species diversity, thus constituting key factors for the similarity/dissimilarity of AMF communities in the M. tenuiflora root zone among sites.

  11. Preliminary phytochemical and elemental analysis of aqueous and fractionated pod extracts of Acacia nilotica (Thorn mimosa

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    Mohammed Shaibu Auwal

    2014-06-01

    Full Text Available Acacia nilotica (Thorn mimosa is used locally for various medicinal purposes by traditionalists and herbalists in northeastern Nigeria. Plants products have been used since ancient times in the management of various conditions. The bark of A. nilotica has been reported to be used traditionally to manage diabetes, dysentery, leprosy, ulcers, cancers, tumor of the eye, ear and testicles, induration of liver and spleen and also in treatment of various condylomas. The objective of this study is to determine the phytochemical and elemental constituents of the extracts of A. nilotica pods. Flame emission and atomic absorption spectrometry were also used to determine the presence or absence of micro- and macro-elements in the extracts. Phytochemical analysis of the aqueous, ethyl acetate and N-butanol fractionated portions of the pod extracts of A. nilotica revealed the presence of tannins, saponins, flavonoids, carbohydrate, whereas carbohydrates and tannins were the only constituent in the residue portion. Anthraquinones, alkaloids, terpene and steroids were not present in the extracts. The elemental screening revealed the presence of iron, potassium, manganese, zinc, calcium, phosphorous, magnesium, sodium, cadmium and copper. Lead, arsenic and molybdenum were not detected in the pod.

  12. Arbuscular mycorrhizal fungi in Mimosa tenuiflora (Willd. Poir from Brazilian semi-arid

    Directory of Open Access Journals (Sweden)

    Tancredo Augusto Feitosa de Souza

    2016-06-01

    Full Text Available Abstract Many plant species from Brazilian semi-arid present arbuscular mycorrhizal fungi (AMF in their rhizosphere. These microorganisms play a key role in the establishment, growth, survival of plants and protection against drought, pathogenic fungi and nematodes. This study presents a quantitative analysis of the AMF species associated with Mimosa tenuiflora, an important native plant of the Caatinga flora. AMF diversity, spore abundance and root colonization were estimated in seven sampling locations in the Ceará and Paraíba States, during September of 2012. There were significant differences in soil properties, spore abundance, percentage of root colonization, and AMF diversity among sites. Altogether, 18 AMF species were identified, and spores of the genera Acaulospora, Claroideoglomus, Dentiscutata, Entrophospora, Funneliformis, Gigaspora, Glomus, Racocetra, Rhizoglomus and Scutellospora were observed. AMF species diversity and their spore abundance found in M. tenuiflora rhizosphere shown that this native plant species is an important host plant to AMF communities from Brazilian semi-arid region. We concluded that: (a during the dry period and in semi-arid conditions, there is a high spore production in M. tenuiflora root zone; and (b soil properties, as soil pH and available phosphorous, affect AMF species diversity, thus constituting key factors for the similarity/dissimilarity of AMF communities in the M. tenuiflora root zone among sites.

  13. Polysaccharide extract of Mimosa tenuiflora stem barks stimulates acute inflammatory response via nitric oxide

    Directory of Open Access Journals (Sweden)

    Kaira Emanuella Sales da Silva-Leite

    2016-12-01

    Full Text Available Mimosa tenuiflora (Mimosaceae or “jurema-preta” is well distributed in the northeast Brazil, being popularly used to treat skin lesions, burns and inflammation. The healing effect of the alcoholic extract prepared with its barks corroborates the popular use. This study aimed to evaluate the inflammatory response of polysaccharides extracted from M. tenuiflora barks (EP-Mt by methanol/NaOH and ethanol precipitation. Inflammatory activity was assessed in rat models of acute inflammation (paw edema and peritonitis, by the following parameters: edema, vascular permeability, leukocyte migration, myeloperoxidase activity and pharmacological modulation of nitric oxide and prostaglandins. EP-Mt presented 3.8% yield, 41% carbohydrate and 0.34% protein. EP-Mt (0.01, 0.1, 1.0 mg kg-1 injected by subcutaneous route elicited paw edema that lasted from 30-420 min, with maximal effect at 1 mg kg-1 (40x vs. saline, and was inhibited by L-NAME (52% and dexamethasone (26%. EP-Mt (1 mg kg-1, via intraperitoneal stimulated leukocytes migration (2.2x, mainly neutrophils (6.5x and MPO activity (96%. The leukocyte migration elicited by EP-Mt was inhibited by dexamethasone (39% and L-NAME (38%. EP-Mt containing high carbohydrate content induces acute inflammation via nitric oxide, which open perspectives of application in pathological conditions of immunosuppression.

  14. WATER AND SODIUM CHLORIDE EFFECTS ON Mimosa Tenuiflora (WILLD. POIRET SEED GERMINATION

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    Ivonete Alves Bakke

    2006-01-01

    Full Text Available Water shortage and saline soils of the Brazilian semi-arid northeastern region are limiting factors to the development of many plants. Jurema preta (Mimosa tenuiflora (Willd. Poiret is a small, multiple use tree that abundantly colonizes unfavorable sites, including environments with severe water stress. This work had the objective of investigating the tolerance of jurema preta seeds to water and salt stresses during germination. Seeds germination in polyethylene glycol (PEG-6000 and sodium chloride (NaCl solutions was analyzed under five different osmotic potentials (0.0; -0.3, -0.6, -0.9 and -1.2MPa, in order to simulate water and salt stress, respectively, in four 100-seed replications for each treatment. Seeds were placed into 10cmx10cmx4cm boxes, and germination accomplished in BOD germinator adjusted to 30oC. The number of germinated seeds was monitored every 24 hours, and percentage and speed of seed germination were generated from these data. Mean percentage germination in the control treatment was ~95%, reducing to 63-53% at -0.9 to -1.2-MPa PEG solutions, and to 27- 9.5% at NaCl solutions at equivalent osmotic potentials. Velocity of germination index was more affected, and decreased up to 1/8 of the control, at -0.6 MPa. Jurema preta seeds showed lower tolerance to NaCl than to water stress, and this species can be classified as a glycophyte.

  15. Mimosa Origami: A nanostructure-enabled directional self-organization regime of materials.

    Science.gov (United States)

    Wong, William S Y; Li, Minfei; Nisbet, David R; Craig, Vincent S J; Wang, Zuankai; Tricoli, Antonio

    2016-06-01

    One of the innate fundamentals of living systems is their ability to respond toward distinct stimuli by various self-organization behaviors. Despite extensive progress, the engineering of spontaneous motion in man-made inorganic materials still lacks the directionality and scale observed in nature. We report the directional self-organization of soft materials into three-dimensional geometries by the rapid propagation of a folding stimulus along a predetermined path. We engineer a unique Janus bilayer architecture with superior chemical and mechanical properties that enables the efficient transformation of surface energy into directional kinetic and elastic energies. This Janus bilayer can respond to pinpoint water stimuli by a rapid, several-centimeters-long self-assembly that is reminiscent of the Mimosa pudica's leaflet folding. The Janus bilayers also shuttle water at flow rates up to two orders of magnitude higher than traditional wicking-based devices, reaching velocities of 8 cm/s and flow rates of 4.7 μl/s. This self-organization regime enables the ease of fabricating curved, bent, and split flexible channels with lengths greater than 10 cm, demonstrating immense potential for microfluidics, biosensors, and water purification applications.

  16. Effect of different substrates on growth of Mimosa bimucronata seedlings inoculate with rhizobium

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    Juliana Müller Freire

    2017-06-01

    Full Text Available The objective of this study was to evaluate the growth response of Mimosa bimucronata (DC O. Kuntze seedlings in nursery conditions to inoculation with rhizobium strains previously selected using different substrates. An experimental design of randomized blocks with split plots was used, testing three substrates (pure organic-containing clay, sand and manure in 1: 1: 1 v: v: v ratio; organomineral mixed with 30% straw and sand with vermiculite in 1: 1 v: v and four N sources (inoculation with strains BR 3461 and BR 3470, control with N fertilization and control without fertilization, totalizing 12 treatments. Height and stem diameter were evaluate after 90 days and shoot, root and nodules dry mass were evaluate after 120 days. Organomineral substrates provided better seedling growth. However, only organic-substrate with straw and sand with vermiculite showed positive responses of plants inoculated with BR3470 strain. The performance of the inoculated seedlings was not higher than that of seedlings fertilized with N. The rate of N applied stimulated nodulation rather than inhibit it.

  17. Indoleamines and calcium channels influence morphogenesis in in vitro cultures of Mimosa pudica L.

    Science.gov (United States)

    Ramakrishna, Akula; Giridhar, Parvatam; Ravishankar, G A

    2009-12-01

    The present article reports the interplay of indoleamine neurohormones viz. serotonin, melatonin and calcium channels on shoot organogenesis in Mimosa pudica L. In vitro grown nodal segments were cultured on MS medium with B5 vitamins containing Serotonin (SER) and Melatonin (MEL) at 100 microM and indoleamine inhibitors viz. serotonin to melatonin conversion inhibitor p-chlorophenylalanine (p-CPA) at 40 microM, serotonin reuptake inhibitor (Prozac) 20 microM. In another set of experiment, calcium at 5 mM, calcium ionophore (A23187) 100 microM, and calcium channel blocker varapamil hydrochloride (1 mM) a calcium chelator EGTA (100 microM) were administered to the culture medium. The percentage of shoot multiplication, endogenous MEL and SER were monitored during shoot organogenesis. At 100 microM SER and MEL treatment 60% and 70% explants responded for shoot multiplication respectively. Medium supplemented with either SER or MEL along with calcium (5 mM) 75%-80% explants responded for organogenesis. SER or MEL along with calcium ionophore (A23187) at 100 microM 70% explants responded for shoot multiplication. p-CPA, prozac, verapamil and EGTA, shoot multiplication was reduced and endogenous pools of SER, MEL decreased by 40-70%. The results clearly demonstrated that indoleamines and calcium channels positively influenced shoot organogenesis in M. pudica L.

  18. Sensitive plant (Mimosa pudica) hiding time depends on individual and state.

    Science.gov (United States)

    Reed-Guy, Sarah; Gehris, Connor; Shi, Meng; Blumstein, Daniel T

    2017-01-01

    The decisions animals make to adjust their antipredator behavior to rapidly changing conditions have been well studied. Inducible defenses in plants are an antipredator behavior that acts on a longer time scale, but sensitive plants, Mimosa pudica, have a much more rapid antipredator response; they temporarily close their leaves when touched. The time they remain closed is defined as hiding time. We studied hiding time in sensitive plants and found that individual plants differed significantly in their hiding times. We then showed that the effect of individual explained substantial variation in hiding time on a short time scale. Finally, on a longer time scale, individuality persisted but the amount of variation attributed to individual decreased. We hypothesized that variation in plant condition might explain this change. We therefore manipulated sunlight availability and quantified hiding time. When deprived of light for 6 h, sensitive plants significantly shortened their hiding times. But when only half a plant was deprived of light, hiding times on the deprived half and light exposed half were not significantly different. This suggests that overall condition best explains variation in sensitive plant antipredator behavior. Just like in animals, sensitive plant antipredator behavior is condition dependent, and, just like in animals, a substantial amount of the remaining variation is explained by individual differences between plants. Thus, models designed to predict plasticity in animal behavior may be successfully applied to understand behavior in other organisms, including plants.

  19. Performance Modeling of Mimosa pudica Extract as a Sensitizer for Solar Energy Conversion

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    M. B. Shitta

    2016-01-01

    Full Text Available An organic material is proposed as a sustainable sensitizer and a replacement for the synthetic sensitizer in a dye-sensitized solar cell technology. Using the liquid extract from the leaf of a plant called Mimosa pudica (M. pudica as a sensitizer, the performance characteristics of the extract of M. pudica are investigated. The photo-anode of each of the solar cell sample is passivated with a self-assembly monolayer (SAM from a set of four materials, including alumina, formic acid, gelatine, and oxidized starch. Three sets of five samples of an M. pudica–based solar cell are produced, with the fifth sample used as the control experiment. Each of the solar cell samples has an active area of 0.3848cm2. A two-dimensional finite volume method (FVM is used to model the transport of ions within the monolayer of the solar cell. The performance of the experimentally fabricated solar cells compares qualitatively with the ones obtained from the literature and the simulated solar cells. The highest efficiency of 3% is obtained from the use of the extract as a sensitizer. It is anticipated that the comparison of the performance characteristics with further research on the concentration of M. pudica extract will enhance the development of a reliable and competitive organic solar cell. It is also recommended that further research should be carried out on the concentration of the extract and electrolyte used in this study for a possible improved performance of the cell.

  20. Actin dynamics mediates the changes of calcium level during the pulvinus movement of Mimosa pudica.

    Science.gov (United States)

    Yao, Heng; Xu, Qiangyi; Yuan, Ming

    2008-11-01

    The bending movement of the pulvinus of Mimosa pudica is caused by a rapid change in volume of the abaxial motor cells, in response to various environmental stimuli. We investigated the relationship between the actin cytoskeleton and changes in the level of calcium during rapid contractile movement of the motor cells that was induced by electrical stimulation. The bending of the pulvinus was retarded by treatments with actin-affecting reagents and calcium channel inhibitors. The actin filaments in the motor cells were fragmented in response to electrical stimulation. Further investigations were performed using protoplasts from the motor cells of M. pudica pulvini. Calcium-channel inhibitors and EGTA had an inhibitory effect on contractile movement of the protoplasts. The level of calcium increased and became concentrated in the tannin vacuole after electrical stimulation. Ruthenium Red inhibited the increase in the level of calcium in the tannin vacuole and the contractile movement of the protoplasts. However, treatment with latrunculin A abolished the inhibitory effect of Ruthenium Red. Phalloidin inhibited the contractile movement and the increase in the level of calcium in the protoplasts. Our study demonstrates that depolymerization of the actin cytoskeleton in pulvinus motor cells in response to electrical signals results in increased levels of calcium.

  1. Sensitive plant (Mimosa pudica hiding time depends on individual and state

    Directory of Open Access Journals (Sweden)

    Sarah Reed-Guy

    2017-07-01

    Full Text Available The decisions animals make to adjust their antipredator behavior to rapidly changing conditions have been well studied. Inducible defenses in plants are an antipredator behavior that acts on a longer time scale, but sensitive plants, Mimosa pudica, have a much more rapid antipredator response; they temporarily close their leaves when touched. The time they remain closed is defined as hiding time. We studied hiding time in sensitive plants and found that individual plants differed significantly in their hiding times. We then showed that the effect of individual explained substantial variation in hiding time on a short time scale. Finally, on a longer time scale, individuality persisted but the amount of variation attributed to individual decreased. We hypothesized that variation in plant condition might explain this change. We therefore manipulated sunlight availability and quantified hiding time. When deprived of light for 6 h, sensitive plants significantly shortened their hiding times. But when only half a plant was deprived of light, hiding times on the deprived half and light exposed half were not significantly different. This suggests that overall condition best explains variation in sensitive plant antipredator behavior. Just like in animals, sensitive plant antipredator behavior is condition dependent, and, just like in animals, a substantial amount of the remaining variation is explained by individual differences between plants. Thus, models designed to predict plasticity in animal behavior may be successfully applied to understand behavior in other organisms, including plants.

  2. The gelsolin/fragmin family protein identified in the higher plant Mimosa pudica.

    Science.gov (United States)

    Yamashiro, S; Kameyama, K; Kanzawa, N; Tamiya, T; Mabuchi, I; Tsuchiya, T

    2001-08-01

    Mimosa pudica L. rapidly closes its leaves and bends its petioles downward when mechanically stimulated. It has been suggested that the actin cytoskeleton is involved in the bending motion since both cytochalasin B and phalloidin inhibit the motion. In order to clarify the mechanism by which the actin cytoskeleton functions in the motion, we attempted to find actin-modulating proteins in the M. pudica plant by DNase I-affinity column chromatography. The EGTA-eluate from the DNase I column contained proteins with apparent molecular masses of 90- and 42-kDa. The 42-kDa band consisted of two closely migrating components: the slower migrating component was actin while the faster migrating components was a distinct protein. The eluate showed an activity to sever actin filaments and to enhance the rate of polymerization of actin, both in a Ca(2+)-dependent manner. Microsequencing of the faster migrating 42-kDa protein revealed its similarity to proteins in the gelsolin/fragmin family. Our results provide the first biochemical evidence for the presence in a higher plant of a gelsolin/fragmin family actin-modulating protein that severs actin filament in a Ca(2+)-dependent manner.

  3. Mechanosensitivity below Ground: Touch-Sensitive Smell-Producing Roots in the Shy Plant Mimosa pudica.

    Science.gov (United States)

    Musah, Rabi A; Lesiak, Ashton D; Maron, Max J; Cody, Robert B; Edwards, David; Fowble, Kristen L; Dane, A John; Long, Michael C

    2016-02-01

    The roots of the shy plant Mimosa pudica emit a cocktail of small organic and inorganic sulfur compounds and reactive intermediates into the environment, including SO2, methanesulfinic acid, pyruvic acid, lactic acid, ethanesulfinic acid, propanesulfenic acid, 2-aminothiophenol, S-propyl propane 1-thiosulfinate, phenothiazine, and thioformaldehyde, an elusive and highly unstable compound that, to our knowledge, has never before been reported to be emitted by a plant. When soil around the roots is dislodged or when seedling roots are touched, an odor is detected. The perceived odor corresponds to the emission of higher amounts of propanesulfenic acid, 2-aminothiophenol, S-propyl propane 1-thiosulfinate, and phenothiazine. The mechanosensitivity response is selective. Whereas touching the roots with soil or human skin resulted in odor detection, agitating the roots with other materials such as glass did not induce a similar response. Light and electron microscopy studies of the roots revealed the presence of microscopic sac-like root protuberances. Elemental analysis of these projections by energy-dispersive x-ray spectroscopy revealed them to contain higher levels of K(+) and Cl(-) compared with the surrounding tissue. Exposing the protuberances to stimuli that caused odor emission resulted in reductions in the levels of K(+) and Cl(-) in the touched area. The mechanistic implications of the variety of sulfur compounds observed vis-à-vis the pathways for their formation are discussed. © 2016 American Society of Plant Biologists. All Rights Reserved.

  4. Membrane effects of 2,4-dichlorophenoxyacetic acid in motor cells of Mimosa pudica L.

    Science.gov (United States)

    Moyen, Christelle; Bonmort, Janine; Roblin, Gabriel

    2007-01-01

    2,4-dichlorophenoxyacetic acid applied to excised leaves of Mimosa pudica L. inhibited in a dose-dependent manner the shock-induced pulvinar movement. This inhibition was negatively correlated with the amount of [(14)C] 2,4-dichlorophenoxyacetic acid present in the vicinity of the motor cells. Although 2,4-dichlorophenoxyacetic acid is a weak acid, its greatest physiological efficiency was obtained with pH values close to neutrality. This observation opens the question of its mode of action which may be through external signaling or following internal transport by a specific anionic form transporter. The effect was related to molecular structure since 2,4-dichlorophenoxyacetic acid>3,4-dichlorophenoxyacetic acid>2,3-dichlorophenoxyacetic acid. An essential target of 2,4-dichlorophenoxyacetic acid action lies at the plasmalemma as indicated by the induced hyperpolarization of the cell membrane. Compared to indole-3-acetic acid and fusicoccin, it induced a complex effect on H(+) fluxes. Applied to plasma membrane vesicles purified from motor organs, 2,4-dichlorophenoxyacetic acid enhanced proton pumping, but, unlike fusicoccin, it did not increase the H(+)-ATPase catalytic activity in our experimental conditions. Taken together, the data suggest that 2,4-dichlorophenoxyacetic acid acts on cell turgor variation and the concomittant ion migration, in particular K(+), by a mechanism involving specific steps compared to indole-3-acetic acid and fusicoccin.

  5. State dependence, personality, and plants: light-foraging decisions in Mimosa pudica (L.).

    Science.gov (United States)

    Simon, Franz W; Hodson, Christina N; Roitberg, Bernard D

    2016-09-01

    Plants make foraging decisions that are dependent on ecological conditions, such as resource availability and distribution. Despite the field of plant behavioral ecology gaining momentum, ecologists still know little about what factors impact plant behavior, especially light-foraging behavior. We made use of the behavioral reaction norm approach to investigate light foraging in a plant species that exhibits rapid movement: Mimosa pudica. We explored how herbivore avoidance behavior in M. pudica (which closes its leaflets temporarily when disturbed) is affected by an individual's energy state and the quality of the current environment and also repeatedly tested the behavior of individuals from two seed sources to determine whether individuals exhibit a "personality" (i.e., behavioral syndrome). We found that when individuals are in a low-energy state, they adopt a riskier light-foraging strategy, opening leaflets faster, and not closing leaflets as often in response to a disturbance. However, when plants are in a high-energy state, they exhibit a plastic light-foraging strategy dependent on environment quality. Although we found no evidence that individuals exhibit behavioral syndromes, we found that individuals from different seed sources consistently behave differently from each other. Our results suggest that plants are capable of making state-dependent decisions and that plant decision making is complex, depending on the interplay between internal and external factors.

  6. Early membrane events induced by salicylic acid in motor cells of the Mimosa pudica pulvinus.

    Science.gov (United States)

    Saeedi, Saed; Rocher, Françoise; Bonmort, Janine; Fleurat-Lessard, Pierrette; Roblin, Gabriel

    2013-04-01

    Salicylic acid (o-hydroxy benzoic acid) (SA) induced a rapid dose-dependent membrane hyperpolarization (within seconds) and a modification of the proton secretion (within minutes) of Mimosa pudica pulvinar cells at concentrations higher than 0.1mM. Observations on plasma membrane vesicles isolated from pulvinar tissues showed that SA acted directly at the membrane level through a protonophore action as suggested by the inhibition of the proton gradient and the lack of effect on H(+)-ATPase catalytic activity. Comparative data obtained with protonophores (carbonylcyanide-m-chlorophenylhydrazone and 2,4-dinitrophenol) and inhibitors of ATPases (vanadate, N,N'-dicyclohexylcarbodiimide, and diethylstilbestrol) corroborated this conclusion. Consequently, the collapse of the proton motive force led to an impairment in membrane functioning. This impairment is illustrated by the inhibition of the ion-driven turgor-mediated seismonastic reaction of the pulvinus following SA treatment. SA acted in a specific manner as its biosynthetic precursor benzoic acid induced much milder effects and the m- and p-OH benzoic acid derivatives did not trigger similar characteristic effects. Therefore, SA may be considered both a membrane signal molecule and a metabolic effector following its uptake in the cells.

  7. Potensi Anthelmintik Akar Tanaman Putri Malu (Mimosa pudica L. terhadap Hymenolepis nana pada Mencit

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    A. A. Candra

    2008-08-01

    Full Text Available The objective of this experiment was to observe the anthelmintic effect of different concentration of root extract of the sensitive plant (Mimosa pudica L. to Hymenolepis nana (Hymenolepis sp. in mice. Sixty mice were divided into 6 groups consisting of 10 mice per group. Mice were infected with 100 infective eggs of Hymenolepis sp. after deworming by mebendazol. After reaching the prepaten phase ( 21st day, mice were treated with different concentration of root extract per oral, namely 100%, 50%, 25%, 12.5%. Positive control treated with mebendazole and negative control treated with distilled water. Fecal eggs were counted using McMaster method on day 2, 4, 6 and 8 after treatment. Mice were sacrificed for worm counting of Hymenolepis sp. in mice intestine on the day 10 day after treatment. Efication of the root extract to Hymenolepis sp. in mice for concentration 100%, 50%, 25% and 12.5% were 59.62%, 86.38%, 45.54%, 92.49% respectively. Reducing in the number of Hymenolepis sp. was inconsistency decreasing in the eggs number per gram fecal.

  8. Morphing structures and signal transduction in Mimosa pudica L. induced by localized thermal stress.

    Science.gov (United States)

    Volkov, Alexander G; O'Neal, Lawrence; Volkova, Maia I; Markin, Vladislav S

    2013-10-15

    Leaf movements in Mimosa pudica, are in response to thermal stress, touch, and light or darkness, appear to be regulated by electrical, hydrodynamical, and chemical signal transduction. The pulvinus of the M. pudica shows elastic properties. We have found that the movements of the petiole, or pinnules, are accompanied by a change of the pulvinus morphing structures. After brief flaming of a pinna, the volume of the lower part of the pulvinus decreases and the volume of the upper part increases due to the redistribution of electrolytes between these parts of the pulvinus; as a result of these changes the petiole falls. During the relaxation of the petiole, the process goes in the opposite direction. Ion and water channel blockers, uncouplers as well as anesthetic agents diethyl ether or chloroform decrease the speed of alert wave propagation along the plant. Brief flaming of a pinna induces bidirectional propagation of electrical signal in pulvini. Transduction of electrical signals along a pulvinus induces generation of an action potential in perpendicular direction between extensor and flexor sides of a pulvinus. Inhibition of signal transduction and mechanical responses in M. pudica by volatile anesthetic agents chloroform or by blockers of voltage gated ion channels shows that the generation and propagation of electrical signals is a primary effect responsible for turgor change and propagation of an excitation. There is an electrical coupling in a pulvinus similar to the electrical synapse in the animal nerves. Copyright © 2013 Elsevier GmbH. All rights reserved.

  9. Anti-mumps virus activity by extracts of Mimosa pudica, a unique Indian medicinal plant.

    Science.gov (United States)

    Malayan, Jeevan; Selvaraj, Balaji; Warrier, Aparna; Shanmugam, Sambantham; Mathayan, Manikannan; Menon, Thangam

    2013-09-01

    Mumps is an acute and self-limiting disease characterized by parotitis, however in some cases it leads to aseptic meningitis, deafness, encephalitis and orchitis, which is a serious health concern. MMR vaccination was successful in eradicating the disease however, recent reports question the efficacy of MMR vaccine and countless outbreaks are observed in vaccinated populations throughout the world. Lack of specific treatment methods for mumps infection and inefficiency of MMR vaccine in vaccinated populations accentuates the need for the development of novel drugs to control mumps virus mediated serious infections. It was with this backdrop of information that the anti-mumps virus activity of Mimosa pudica was evaluated. Suspected mumps cases were collected to isolate a standard mumps virus by systematic laboratory testing which included IgM antibody assays, virus isolation, RT-PCR and phylogenetic analysis. The virus was quantified by TCID50 assay and anti-mumps virus property was evaluated by CPE reduction assay and cytotoxicity of the extract was measured by MTT assay and phytochemical analysis was done by gas chromatography-mass spectroscopy. The RT-PCR and phylogenetic tree analysis of the SH gene sequence of the clinical isolate showed it to be mumps virus genotype C. 150 μg/ml concentration of M. pudica completely inhibited mumps virus and the drug was found to be non-toxic up to 2 mg/ml. M. pudica was thus found to be a potent inhibitor of MuV.

  10. X-ray CT and histological imaging of xylem vessels organization in Mimosa pudica.

    Science.gov (United States)

    Lee, Sang Joon; Song, Kahye; Kim, Hae Koo; Park, Joonghyuk

    2013-11-01

    Mimosa pudica has three distinct specialized organs, namely, pulvinus, secondary pulvinus, and pulvinule, which are respectively controlling the movements of petioles, leaflets, and pinna in response to external stimuli. Water flow is a key factor for such movements, but detailed studies on the organization of the vascular system for water transport in these organs have not been published yet. In this study, organizations of the xylem vessels and morphological features of the pulvinus, the secondary pulvinus, and the pulvinule were experimentally investigated by X-ray computed tomography and histological technique. Results showed that the xylem vessels were circularly distributed in the specialized motile organs and reorganized into distinct vascular bundles at the extremities. The number and the total cross-sectional area of the xylem vessels were increased inside the specialized motile organs. Morphological characteristics obtained in this study provided new insight to understand the functions of the vascular networks in the dynamic movements of M. pudica. Copyright © 2013 Wiley Periodicals, Inc.

  11. Mimosa Origami: A nanostructure-enabled directional self-organization regime of materials

    Science.gov (United States)

    Wong, William S. Y.; Li, Minfei; Nisbet, David R.; Craig, Vincent S. J.; Wang, Zuankai; Tricoli, Antonio

    2016-01-01

    One of the innate fundamentals of living systems is their ability to respond toward distinct stimuli by various self-organization behaviors. Despite extensive progress, the engineering of spontaneous motion in man-made inorganic materials still lacks the directionality and scale observed in nature. We report the directional self-organization of soft materials into three-dimensional geometries by the rapid propagation of a folding stimulus along a predetermined path. We engineer a unique Janus bilayer architecture with superior chemical and mechanical properties that enables the efficient transformation of surface energy into directional kinetic and elastic energies. This Janus bilayer can respond to pinpoint water stimuli by a rapid, several-centimeters-long self-assembly that is reminiscent of the Mimosa pudica’s leaflet folding. The Janus bilayers also shuttle water at flow rates up to two orders of magnitude higher than traditional wicking-based devices, reaching velocities of 8 cm/s and flow rates of 4.7 μl/s. This self-organization regime enables the ease of fabricating curved, bent, and split flexible channels with lengths greater than 10 cm, demonstrating immense potential for microfluidics, biosensors, and water purification applications. PMID:28861471

  12. Phytoremediation of Petroleum Hydrocarbon (PHC) Contaminated Soil by Using Mimosa pudica L. .

    Science.gov (United States)

    Budhadev, Basumatary; Rubul, Saikia; Sabitry, Bordoloi; Hari Prasad, Sarma

    2014-07-01

    The aim of this study was to evaluate the efficiency of Mimosa pudica L. that could be effective in phytoremediation of PHC-contaminated soil. Experiments were conducted in net house to determine the tolerance of this species to a heavy crude oil contaminated soil under the application of two fertilizer levels and reduction of PHC was monitored for 180 days. Assessment of plant growth, biomass and Total Oil and Grease (TOG) degradation were carried out at an interval of 60 days. In the presence of contaminants, biomass and plant height were reduced up to 27% and 10.4% respectively. Experiments with different percentages of crude oil showed that M. pudica could tolerate crude-oil contamination up to 6.2% (w/w). The estimation of TOG in soil of the tested plants revealed that M. pudica could decrease 31.7% of crude oil contaminants in low fertilizer level (200N, 100P, 100K) and 24.7% in high fertilizer level (240N, 120P, 120K). In case of unplanted pots, the reduction of TOG was 13.7% in low fertilizer level and 11.2% in high fertilizer level. This experiment has identified the suitability of a native candidate plant species for further investigation of their phytoremediation potential.

  13. Diterpene esters and phenolic compounds from Sapium insigne (ROYLE) BENTH. ex HOOK. fil.

    Science.gov (United States)

    Devkota, Hari Prasad; Basnet, Purusotam; Yahara, Shoji

    2009-11-01

    From the leaves of Sapium insigne (ROYLE) BENTH. ex HOOK. fil., two new phorbol derivatives, such as 16-hydroxyphorbol-16-acetate (4) and 4beta-deoxy-16-hydroxyphorbol-16-acetate (5) along with twelve known phenolic compounds such as 3-O-caffeoyl quinic acid (1), 1-O-galloyl-beta-D-glucose (2), 1,3-di-O-galloyl-beta-D-glucose (3), rutin (6), 1,3,4,6-tetra-O-galloyl-beta-D-glucose (7), quercetin (8), guaijaverin (9), nicotiflorin (10), scopolin (11), methyl gallate (12), corilagin (13) and 1,3,6-tri-O-galloyl-beta-D-glucose (14) were isolated. All of these compounds have been isolated for the first time from this plant.

  14. Pyrolysis of Parinari polyandra Benth fruit shell for bio-oil production

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    Temitope E. Odetoye

    2014-09-01

    Full Text Available Non-conventional agricultural residues such as Parinari polyandra Benth fruit shell (PPBFS are potential sources of biomass feedstock that have not been investigated for bio oil production. In this study, PPBFS was pyrolyzed via an intermediate pyrolysis process for the production of bio oil. The bio oils were obtained using a fixed bed reactor within a temperature range of 375–550 oC and were characterized to determine their physicochemical properties. The most abundant organic compounds present were acetic acid, toluene, 2-cyclopenten-1-one, 2-furanmethanol, phenol, guaiacol and 2,6-dimethoxyphenol. The bio-oil produced at 550 oC possessed a higher quantity of desirable compounds than those produced at lower temperatures. The presence of acetic acids in the bio-oil suggested the need to upgrade the bio-oil before utilization as a fuel source.

  15. Peningkatan kadar patchouli alcohol pada minyak nilam (Pogostemon cablin Benth dengan metode distilasi fraksinasi vakum

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    Rahma Isfaroiny

    2012-02-01

    Full Text Available The quality of nilam (Pogostemon cablin Benth oil depend on it patchouli alcohol value. At there time nilam farmers just has produced nilam oil with patchouli alcohol value about 26–28%. To increase this value, fractional vacuum distillation had been done in this study. Nilam oil has been isolated from drying leaf of nilam plant. Patchouli alcohol in this oil was analyzed by gas chromatography, next the oil was distillated by fractional vacuum and the patchouli alcohol was determined from each fraction. This study showed that fractional vacuum distillation of nilam oil gives a higher patchouli alcohol value. The increasing was 23.3387%; from 35.022% at nilam oil before fractination vacuum distillation to 58.3515% at the third fraction from fractional vacuum distillation. In fact, the fourth fraction had the highest patchouli alcohol, but it had dark colour so isn’t fill the standard quality.

  16. Anti-bacterial activity of the methanolic extract of leucas hyssopifolia (Benth.

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    Devendra Mishra

    2013-01-01

    Full Text Available Context: Methanolic extract of Leucas hyssopifolia roots was investigated for its anti-bacterial property. Aim: Evaluation of anti-bacterial activity of Leucas hyssopifolia Benth. Settings and Design: Roots of the plant were collected, extracted and finally evaluated for their anti-bacterial activity. Materials and Methods: Paper disc diffusion method and microdilution technique were employed for the determination of zone of inhibition and minimal inhibitory concentration, respectively. Results: The extract showed anti-bacterial activity against all the tested bacterial strains except Escherichia coli. Conclusions: Anti-bacterial activity of extract of Leucas hyssopifolia roots may be due to the presence of secondary plant metabolites like terpenoids, steroids and flavonoids, which are present in the extract. The extract can be further studied for the isolation of chemical compounds and their biological activity.

  17. Antimicrobial effects of the stem bark extracts of Parkia biglobosa (Jacq.) Benth. on Shigellae.

    Science.gov (United States)

    Millogo-Kone, H; Guissou, Ip; Nacoulma, O; Traore, A S

    2007-06-10

    Total and hydroalcoholic extracts of the stem barks of Parkia biglobosa (Jacq) Benth. (Mimosaceae) were tested on strains belonging to three species of Shigellae: S. dysenteriae, S. flexneri and S. boydii collected from hospitals in Ouagadougou, Burkina Faso. The results showed that both extracts were active against Shigellae. The hydroalcoholic extract was more active than the decoction (aqueous one) prescribed by the traditional healer. Both extracts were particularly effective against S. dysenteriae, the most virulent of the three pathogenic species. The effects of the extracts have been compared to that of gentamicin. The phytochemical screening on the extracts revealed the presence of sterols, triterpenes, polyphenolic compounds including tannins, flavonoids, coumarins, anthocyanidins. Other components are saponosides and reducing sugars.

  18. Licanol, um novo flavanol, e outros constituintes de Licania macrophylla Benth

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    Fernando Antônio de Medeiros

    2012-01-01

    Full Text Available The phytochemical investigation of L. macrophylla Benth led to the isolation of a new flavanol named licanol: (--4'-O-methyl-epigallocatechin-3'-O-α-L-rhamnoside, along with nine known compounds, identified as: (--4'-O-methyl-epigallocatechin, pheophytin A, 13²-hydroxy-(13²-S-pheophytin A, pheophytin B, sitosterol, stigmasterol, sitosterol-β-O-glucoside, betulinic alcohol and oleanolic acid. The structures were established based on IR, HR-ESI-MS, and NMR spectrometric data analysis with the aid of 2D techniques. The methanolic extracts of leaves and stem bark as well as the compounds licanol, 13²-hidroxi-(13²-S-feofitina A, and betulinic alcohol demonstrated antimicrobial activity against several bacterial strains.

  19. Nothoapiole and α -Asarone Rich Essential Oils from Himalayan Pleurospermum angelicoidesBenth

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    Chandra S. Mathela

    2015-06-01

    Full Text Available Pleurospermum angelicoides Benth. , a herb used in folk medicine, was collected from near Milam glacier of Uttarakhand. Different plant parts were investigated for their terpenoid compositions, antifungal, antibacterial and antioxidant potential. Nothoapiole (5-allyl-4,6,7-trimethoxy-benzo[1,3] dioxide 1 was observed as a single major constituent (87.3% in the root essential oil while α -asarone was the major constituent of leaf and flower essential oils (23.2%, 20.7% respectively. Monoterpenes viz. limonene, α-pinene, γ-terpinene and perilla aldehyde were other constituents of leaf and flower oils. All the essential oils exhibited broad range of antioxidant potential. The leaf essential oil showed significant antifungal activity against Candida albicans and Candida glabrata.

  20. Uma nova lignana e outros constituintes químicos de Hypenia salzmannii (Benth.) Harley (Lamiaceae)

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    Lucena, Hellane Fabricia Sousa de

    2012-01-01

    A família Lamiaceae possui 7.193 espécies distribuidas em aproximadamente 260 gêneros que ocorrem na forma de ervas, arbustos e árvores distribuídos em ambos os hemisférios e inclui um grande número de plantas medicinais de importância significativa. No Brasil ocorrem aproximadamente de 26 gêneros e 350 espécies. O gênero Hypenia (Mart. ex Benth) R. Harley possui distribuição restrita na América do Sul com aproximadamente 27 espécies distribuídas em algumas regiões da Venezuela, Paraguai, Bol...

  1. EFFECT OF THE ORTHOSIPHON STAMINEUS, BENTH ON AMINOPYRINE METABOLISM IN RAT HEPATOCYTES

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    CHIN JIN HAN1

    2007-01-01

    Full Text Available One of the commonly used herbal medicines in Malaysia is Orthosiphon stamineus, Benth (family: Lamiaceae or locally known as Misai Kucing. This experiment was undertaken to evaluate possible interaction of methanol extract of O. stamineus with aminopyrine, a model drug, in different age groups (young, adult and old of Sprague-Dawley (SD female rat hepatocytes. Hepatocytes were prepared by collagenase perfusion technique. Determination of aminopyrine N-demethylase activity was done by measuring formaldehyde formed. From these findings, only normal young female rat hepatocytes in the presence of 0.001 mg/ml of methanol extract of O. stamineus showed significant increase in aminopyrine N-demethylase activity. However, aminopyrine N-demethylase activity was not affected in hepatocytes of normal adult and old female SD rats. In conclusion, exposure of methanol extract of O. stamineus could affect phase I aminopyrine metabolism in normal young female SD rat hepatocytes but this effect was age-dependent.

  2. RP-HPLC method for the quantitation of β-Sitosterol in Elaeagnus Gonyanthes Benth

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Objective To establish an RP-HPLC method for the determination of β-Sitosterol in Elaeagnus Gonyanthes Benth.Methods The separation was performed on a luna C8(2)(150 mm×4.6 mm,5μm)column with the mobile phase of methanol-water(88∶12,v/v)at a flow rate of 1.0 mL/min,the detection wavelength was set at 210 nm,and the temperature of the column was maintained at 35 ℃.Results The calibration curve of β-Sitosterol was linear over the concentration range of 0.075-0.375 mg/mL(r=0.9999)and the average recovery of β-...

  3. Evaluation of anti-inflammatory activity of Strobilanthus callosus Nees and Strobilanthus ixiocephala Benth

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    Rupali Vitthal Sarpate

    2012-01-01

    Full Text Available Context: Strobilanthus callosus Nees and Strobilanthus ixiocephala Benth belongs to family Acanthaceae. The plants have been the subject of scientific research which confirms its use in folk medicine as anti-inflammatory drugs showing potent anti-rheumatic effects. Previous research claims the anti-inflammatory and anti-arthritic activities of Lupeol and 19α-H Lupeol isolated from Strobilanthus callosus and Strobilanthus ixiocephala roots. Based on the literature cited, the unexplored parts stems and leaves of the two species were selected for the present study. Aim: The present study is designed to isolate steroidal and alkaloidal components from the two species Strobilanthus callosus and Strobilanthus ixiocephala using the unexplored parts viz. stems and leaves and to investigate its anti-inflammatory effect. Settings and Design: The anti-inflammatory effect was investigated employing subacute anti-inflammatory models namely cotton pellet granuloma and carrageenan-induced rat paw edema. Materials and Methods: Anti-inflammatory activity was carried out using isolated test components RVS-A (Lupeol, RVS-C (Doctriacantone and standard drug Diclofenac sodium (10 mg/kg. Results: The present study has dealt up with isolation of two phytoconstituents Lupeol and Dotriacontane which gave marked anti-inflammatory activity at the dose 20 mg/kg in both the models Carrageenan induced rat paw edema and Cotton pellet granuloma. Conclusion: The results confirm that the mechanism of the anti-inflammatory effect of RVS-A (Lupeol and RVS-C (Doctriacantone involves reduction of prostaglandins through inhibition of cyclooxygenase and suppression of proliferative phase of sub acute inflammation. Thus the steroidal and alkaloidal components Lupeol and Doctriacantone isolated from Strobilanthus callosus Nees and Strobilanthus ixiocephala Benth shows marked anti-inflammatory activity.

  4. Avaliação das atividades citotóxica e genotóxica de Taninos de Mimosa arenosa (Willd.) Poir. (MIMOSACEAE)

    OpenAIRE

    Goncalves, Gregorio Fernandes

    2011-01-01

    Os produtos naturais representam uma rica fonte de compostos biologicamente ativos e são um exemplo de diversidade molecular, com reconhecido potencial na descoberta e desenvolvimento de novos medicamentos. O gênero Mimosa se distribui nos mais variados ambientes e nos diversos tipos de vegetação das regiões tropicais. No nordeste do Brasil pode ser encontrado nos estados da Bahia, Pernambuco e Paraíba. Muitas espécies de Mimosa são economicamente importantes, no entanto, po...

  5. Screening of Ethanol, Petroleum Ether and Chloroform Extracts of Medicinal Plants, Lawsonia inermis L. and Mimosa pudica L. for Antibacterial Activity.

    Science.gov (United States)

    Akter, A; Neela, F A; Khan, M S I; Islam, M S; Alam, M F

    2010-05-01

    Organic extracts (ethanol, petroleum ether and chloroform) of two medicinal plants Lawsonia inermis L. and Mimosa pudica L. were proven for antibacterial properties against 15 Gram-positive and Gram-negative human pathogenic bacteria. Among the three types of extracts tested, ethanol extract was found to possess maximum antibacterial activity. The diameter of the zone of inhibition of bacterial growth showed that Gram-negative bacteria are more sensitive than Gram-positive bacteria to plant extracts. Between the two plants species studied, Lawsonia inermis extract showed more antibacterial activity compared to Mimosa pudica extract.

  6. Phytochemical Analysis and Antimicrobial Activities of Methanolic Extracts of Leaf, Stem and Root from Different Varieties of Labisa pumila Benth

    OpenAIRE

    Ehsan Karimi; Jaafar, Hawa Z.E.; Sahida Ahmad

    2011-01-01

    A local herb, Kacip Fatimah, is famous amongst Malay women for its uses in parturition; however, its phytochemical contents have not been fully documented. Therefore, a study was performed to evaluate the phenolics, flavonoids, and total saponin contents, and antibacterial and antifungal properties of the leaf, stem and root of three varieties of Labisia pumila Benth. Total saponins were found to be higher in the leaves of all three varieties, compared to the roots and stems. Leaves of var. p...

  7. Activités insecticides de Striga hermonthica (Del.) Benth (Scrophulariaceae) sur Callosobrichus maculatus (Fab.) (Coleoptera : Bruchidae)

    OpenAIRE

    Nacoulma OG.; Ouedraogo AP.; Kiendrebeogo M.

    2006-01-01

    Insecticidal activities of Striga hermonthica (Del.) Benth (Scrophulariacecae) on Callobruchus maculatus (Fab.) (Coleptera Bruchidae). This paper deals with insecticidal potentialities of Striga hermonthica (Del.) (Scrophulariaceae) in protection of cowpea Vigna unguculata (L.) Walp against Callosobruchus maculatus (Fab.) (Coleoptera: Bruchidae) during storage. Crude acetone extract at 0,5% w/w (100 mg of extract for 20 g of grain) exhibits 48% of ovicidal effect and then reduces by half emer...

  8. Effect of Mimosa pudica root powder on oestrous cycle and ovulation in cycling female albino rat, Rattus norvegicus.

    Science.gov (United States)

    Valsala, S; Karpagaganapathy, P R

    2002-03-01

    Mimosa pudica root powder (150 mg/kg body weight) when administered intragastrically, altered the oestrous cycle pattern in female Rattus norvegicus. Nucleated and cornified cells were absent in all rats. The smear was characterized by leucocytes only, as in dioestrus, which persisted for 2 weeks. There was a significant reduction in the number of normal ova in rats treated with the root powder compared with the control rats, and a significant increase in the number of degenerated ova. Copyright 2002 John Wiley & Sons, Ltd.

  9. A Mathematical Model on Water Redistribution Mechanism of the Seismonastic Movement of Mimosa Pudica

    Science.gov (United States)

    Kwan, K.W.; Ye, Z.W.; Chye, M.L.; Ngan, A.H.W.

    2013-01-01

    A theoretical model based on the water redistribution mechanism is proposed to predict the volumetric strain of motor cells in Mimosa pudica during the seismonastic movement. The model describes the water and ion movements following the opening of ion channels triggered by stimulation. The cellular strain is related to the angular velocity of the plant movement, and both their predictions are in good agreement with experimental data, thus validating the water redistribution mechanism. The results reveal that an increase in ion diffusivity across the cell membrane of <15-fold is sufficient to produce the observed seismonastic movement. PMID:23823246

  10. Caracterização e ontogenia dos tricomas glandulares de Ocimum selloi Benth. - Lamiaceae Characterization and ontogeny of the glandular trichomes of Ocimum selloi Benth. (Lamiaceae

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    Letícia de Almeida Gonçalves

    2010-12-01

    Full Text Available Ocimum selloi Benth. (Lamiaceae é uma espécie nativa da América do Sul e na medicina popular tem sido usada devido suas propriedades analgésica, anti-inflamatória e antiespasmódica. O objetivo do presente trabalho foi identificar os tipos de tricomas glandulares que ocorrem nos órgãos vegetativos e reprodutivos de O. selloi e determinar a ontogenia desses tricomas. Ramos laterais em início de formação, folhas totalmente expandidas, flores em diferentes estádios de diferenciação, amostras de caule e do eixo das inflorescências foram analisados em microscopias de luz e eletrônica de varredura. Tricomas glandulares do tipo peltado e capitado subséssil foram observados no caule, nas folhas, no eixo da inflorescência e na superfície adaxial das sépalas. Nas sépalas foi encontrado, além dos tricomas secretores peltados e capitados subsésseis, o tricoma glandular capitado pedunculado. A ontogenia inicia-se com a expansão de uma célula protodérmica que, de acordo com a seqüência de divisões periclinais e anticlinais (ora simétricas, ora assimétricas, dá origem aos tricomas. A diferenciação dos tricomas glandulares peltados e capitados não é sincrônica e ocorre muito cedo no desenvolvimento da folha, do caule e do eixo floral.Ocimum selloi Benth. (Lamiaceae is native to South America and in traditional medicine has been used due to its analgesic, anti-inflammatory, and antispasmodic properties. The aim of this study was to identify the types of glandular trichomes that occur on the vegetative and reproductive organs of O. selloi and to determine trichome ontogeny. Lateral branches at the initial formation phase, fully opened leaves, flowers at different differentiation stages, and stem and inflorescence axes were analyzed under light and scanning electron microscopy. Glandular trichomes of the peltate and subsessile capitate types were observed on the stem, leaves, inflorescence axis and the adaxial surface of the

  11. LICOR DE MORA DE CASTILLA (Rubus glaucus Benth CON DIFERENTES PORCENTAJES DE PULPA BLACKBERRY LIQUOR (Rubus glaucus Benth WITH DIFFERENT PULP PERCENTAGES

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    Álvaro Montoya Gómez

    2005-12-01

    Full Text Available La mora de Castilla (Rubus glaucus Benth es una fruta de interés comercial y altamente perecedera, que presenta deterioro debido a su fragilidad e inadecuado manejo poscosecha, cantidades apreciables de fruta son afectadas, básicamente en sus características físicas, siendo este producto apto para el procesamiento industrial. Veinte kg de mora fueron despulpados, homogenizados y caracterizada la pulpa, determinando; grados brix de 6,0; porcentaje de acidez de 2,91 % y densidad de 0,991 g/ml en promedio. Se formularon licores, con una participación de la pulpa del 25 %, 30 %, 35 %, 40 %, 45 %, 50 % y 55 % iniciando con 35 °Brix, para todos los casos en el mosto fermentable, ajustando las formulaciones con sacarosa; la levadura inoculada fue Saccharomyces cerevisiae Meyen ex E.C. Hansen en concentración del 0,2 %; la fermentación se llevó a cabo en reactores con capacidad de 2 litros, con desfogue de manguera de látex, el mosto fermentable fue dejado en los reactores durante 20 semanas, luego los licores fueron filtrados y caracterizados físico-químicamente, evaluando el porcentaje de alcohol por destilación, encontrando que el porcentaje de 40 % de participación de pulpa, fue el de mayor concentración con 8,36 % de volumen de alcohol en promedio. Los resultados estadísticos mostraron una diferencia significativa entre los porcentajes de participación de pulpa y su influencia sobre la producción de alcohol para los valores de 25 % y 30 % con respecto a los porcentajes 35 %, 40 %, 45 %, 50 % y 55 %. Un modelo matemático lineal apropiado para la producción de alcohol en función de la participación de pulpa fue obtenido. La prueba sensorial con 10 jueces, mostró que el licor preparado con 35 % de participación de pulpa, fue el de mayor aceptación.The blackberry (Rubus glaucus Benth is a fruit of commercial interest that is highly perishable, showing appreciable damage due to fragileness and inadequate pos-harvesting handling

  12. Antioxidant and antifungal activities of extracts and condensed tannins from Stryphnodendron obovatum Benth. Atividades antioxidante e antifúngica de extratos e taninos condensados de Stryphnodendron obovatum Benth.

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    Andréia Cristina Conegero Sanches

    2005-03-01

    Full Text Available The antioxidant activity of stem-bark extracts from Stryphnodendron obovatum Benth., including fractions and isolated compounds, was evaluated by DPPH in thin-layer chromatography. All the fractions and isolated compounds showed antioxidant activity. Antifungal activity was determined by the minimum inhibitory concentration (MIC and minimum fungicidal concentration (MFC against the yeasts Candida albicans, Candida parapsilosis, Candida krusei and Candida tropicalis. All extracts (CE, EtOAc and FW, subfractions (F1-F12 and the compounds I, II and III were inactive against the yeasts. Against C. parapsilosis and C. albicans, fractions F13-15 and F20 showed moderate antifungal activity, and fractions F16-19 and F21-22 showed good activity. Chemical isolation of the ethyl-acetate fraction resulted in the identification of three compounds: epigallocatechin, gallocatechin and epigallocatechin-(4b®8-gallocatechin.Atividade antioxidante de extrato, frações, subfrações e substâncias isoladas das cascas de Stryphnodendron obovatum Benth. foi avaliada através da redução do radical 1,1-difenil-2-picrilhidrazila (método DDPH· em cromatografia em camada delgada. O extrato bruto (CE, acetona:água, as frações acetato de etila (EtOAc e aquosa (FW, as subfrações (F1-F12 e as substâncias isoladas I, II e III apresentaram a capacidade de reduzir o radical DDPH·. A atividade antifúngica foi determinada pela concentração inibitória mínima (CIM e concentração fungicida mínima (CFM frente às amostras de leveduras Candida albicans, Candida parapsilosis, Candida krusei e Candida tropicalis. O extrato bruto (CE, as frações (EtOAC e FW, e os compostos isolados I, II e III, como também as subfrações cromatográficas (F1-F12 foram inativos frente a todas as leveduras testadas. Por outro lado, as subfrações cromatográficas F13-15 e F20 apresentaram atividade antifúngica moderada. Já as subfrações F16-19 e F21-22 mostraram boa atividade

  13. Isolation of Fonsecaea pedrosoi from thorns of Mimosa pudica, a probable natural source of chromoblastomycosis Isolamento de Fonsecaea pedrosoi a partir de espinhos da planta Mimosa pudica, uma provável fonte natural de cromoblastomicose

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    Claudio Guedes Salgado

    2004-02-01

    Full Text Available We report the isolation of Fonsecaea pedrosoi from thorns of the plant Mimosa pudica L. at the place of infection identified by one of our patients. Clinical diagnosis of chromoblastomycosis was established by direct microscopic examination and cultures from the patient's lesion. The same species was isolated from the patient and from the plant. Scanning electron microscopy of the surface of the thorns showed the characteristic conidial arrangement of F. pedrosoi. These data indicate that M. pudica could be a natural source of infection for the fungus F. pedrosoi.Este trabalho demonstra o isolamento de Fonsecaea pedrosoi de espinhos da planta Mimosa pudica L., a partir do local de suposta infecção identificado pela paciente infectada. O diagnóstico clínico de cromoblastomicose foi estabelecido pelo achado de corpos fumagóides no exame microscópico direto e pelas culturas de F. pedrosoi do material obtido da lesão da paciente. A mesma espécie foi isolada da paciente e da planta. A microscopia eletrônica de transmissão da superfície dos espinhos evidenciou a disposição conidial característica de F. pedrosoi. Estes dados indicam que a planta M. pudica deve ser uma fonte natural de infecção do fungo F. pedrosoi.

  14. Dormancy overcoming, temperatures and substrates on germination of Mimosa tenuiflora Willd seeds

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    Clarisse Pereira Benedito

    2017-03-01

    Full Text Available Mimosa tenuiflora Willd., popularly known as jurema-preta, is an arboreal species of great importance for the brazilian Northeast due to its uses as a medicinal plant and in the restoration of degraded soils. No information is available in the Rules for Seed Analysis and the Instructions for Seed Analysis of Forest Species regarding ideal conditions for of this species. Thus, this study aimed at evaluating the influence of pre-germination treatments, in addition to verifying the germination performance at different temperatures and in different substrates. In both experiments, four replicates of 25 seeds were used for each treatment. In experiment I, seeds were submitted to the following methods for overcoming dormancy: witness - Intact seeds (T1 immersion in water at 100 °C for 1 (T2, 2 (T3, 3 (T4, 4 (T5, 5 (T6 and 6 min (T7, immersion in concentrated sulfuric acid for 1 (T8, 4 (T9, 7 (T10, 10 (T11 and 13 min (T12, scarification on sandpaper n° 80 (T13 and lopping in the region opposite the micropyle (T14. In experiment II, seed germination was evaluated in four types of substrates: between sand, paper on, paper roll and between vermiculite and at six different temperatures: 20, 25, 30, 35, 40 °C and alternating between 20 and 30 °C. Immersion in hot water for 1, 2, 3, 4, 5 and 6 min, sulfuric acid treatment for 10 and 13 min, sand paper and lopping were the most appropriate treatments to overcome seed dormancy. The seed germination of M. Tenuiflora should be carried out at 25 °C on paper roll substrate.

  15. Evaluation of Mimosa pudica seed mucilage as sustained-release excipient.

    Science.gov (United States)

    Singh, Kuldeep; Kumar, Ashok; Langyan, Naresh; Ahuja, Munish

    2009-01-01

    The present study was conducted to investigate the sustained-release properties of Mimosa pudica seed mucilage. Matrix tablets of diclofenac sodium containing different proportions of mucilage and dibasic calcium phosphate as diluent were formulated by wet granulation method. The tablets had uniform physical appearance, average weight, drug content, and adequate hardness. The results of in vitro release conducted using USP type II dissolution rate apparatus, in a dissolution media comprising of 900 mL of 0.1 N HCl for 2 h followed by phosphate buffer (pH 6.8) for 24 h at 37 degrees C and 50 rpm, revealed that as the proportion of mucilage in the matrix was increased there was a corresponding decrease in the release of drug. Further, the matrix tablets were found to release the drug following Higuchi square root release kinetics, with the mechanism of release being diffusion for tablets containing higher proportion of mucilage and a combination of matrix erosion and diffusion for tablets containing smaller proportion of mucilage. The swelling and erosion studies revealed that, as the proportion of mucilage in tablets was increased, there was a corresponding increase in percent swelling and a decrease in percent erosion of tablets. The SEM photomicrographs showed gelling structures in tablets containing higher percentage of mucilage, while both pores and gelling structures were present on the surface of tablets containing smaller proportion of mucilage and commercial formulation. On comparative evaluation, the dissolution profile from formulation containing mucilage to drug in the proportion of 1:40 was found to be similar to the commercial sustained-release formulation of diclofenac.

  16. Purification and biochemical characterization of a novel ecto-apyrase, MP67, from Mimosa pudica.

    Science.gov (United States)

    Okuhata, Riku; Takishima, Takeshi; Nishimura, Naoaki; Ueda, Shogo; Tsuchiya, Takahide; Kanzawa, Nobuyuki

    2011-09-01

    We have previously reported the presence of an apyrase in Mimosa pudica. However, only limited information is available for this enzyme. Thus, in this study, the apyrase was purified to homogeneity. The purified enzyme had a molecular mass of around 67 kD and was able to hydrolyze both nucleotide triphosphate and nucleotide diphosphate as substrates. The ratio of ATP to ADP hydrolysis velocity of the purified protein was 0.01 in the presence of calcium ion, showing extremely high substrate specificity toward ADP. Thus, we designated this novel apyrase as MP67. A cDNA clone of MP67 was obtained using primers designed from the amino acid sequence of trypsin-digested fragments of the protein. In addition, rapid amplification of cDNA ends-polymerase chain reaction was performed to clone a conventional apyrase (MpAPY2). Comparison of the deduced amino acid sequences showed that MP67 is similar to ecto-apyrases; however, it was distinct from conventional apyrase based on phylogenetic classification. MP67 and MpAPY2 were expressed in Escherichia coli, and the recombinant proteins were purified. The recombinant MP67 showed high substrate specificity toward ADP rather than ATP. A polyclonal antibody raised against the recombinant MP67 was used to examine the tissue distribution and localization of native MP67 in the plant. The results showed that MP67 was ubiquitously distributed in various tissues, most abundantly in leaves, and was localized to plasma membranes. Thus, MP67 is a novel ecto-apyrase with extremely high substrate specificity for ADP.

  17. Development of an Agrobacterium-mediated stable transformation method for the sensitive plant Mimosa pudica.

    Science.gov (United States)

    Mano, Hiroaki; Fujii, Tomomi; Sumikawa, Naomi; Hiwatashi, Yuji; Hasebe, Mitsuyasu

    2014-01-01

    The sensitive plant Mimosa pudica has long attracted the interest of researchers due to its spectacular leaf movements in response to touch or other external stimuli. Although various aspects of this seismonastic movement have been elucidated by histological, physiological, biochemical, and behavioral approaches, the lack of reverse genetic tools has hampered the investigation of molecular mechanisms involved in these processes. To overcome this obstacle, we developed an efficient genetic transformation method for M. pudica mediated by Agrobacterium tumefaciens (Agrobacterium). We found that the cotyledonary node explant is suitable for Agrobacterium-mediated transformation because of its high frequency of shoot formation, which was most efficiently induced on medium containing 0.5 µg/ml of a synthetic cytokinin, 6-benzylaminopurine (BAP). Transformation efficiency of cotyledonary node cells was improved from almost 0 to 30.8 positive signals arising from the intron-sGFP reporter gene by using Agrobacterium carrying a super-binary vector pSB111 and stabilizing the pH of the co-cultivation medium with 2-(N-morpholino)ethanesulfonic acid (MES) buffer. Furthermore, treatment of the explants with the detergent Silwet L-77 prior to co-cultivation led to a two-fold increase in the number of transformed shoot buds. Rooting of the regenerated shoots was efficiently induced by cultivation on irrigated vermiculite. The entire procedure for generating transgenic plants achieved a transformation frequency of 18.8%, which is comparable to frequencies obtained for other recalcitrant legumes, such as soybean (Glycine max) and pea (Pisum sativum). The transgene was stably integrated into the host genome and was inherited across generations, without affecting the seismonastic or nyctinastic movements of the plants. This transformation method thus provides an effective genetic tool for studying genes involved in M. pudica movements.

  18. Effects of Mimosa pudica L. leaves extract on anxiety, depression and memory.

    Science.gov (United States)

    Patro, Ganesh; Kumar Bhattamisra, Subrat; Kumar Mohanty, Bijay

    2016-01-01

    The present study was carried out to investigate the neuropharmacological activities of ethyl acetate extract of Mimosa pudica (EAMP) leaves on anxiety, depression and memory in a mouse model. Anti-anxiety potential of EAMP was evaluated by elevated plus maze (EPM), light-dark box (LDB) and social interaction (SI) tests in mice.Anti-depressant potential of EAMP was evaluated by forced swimming (FST), tail suspension (TST), and open field tests (OFT). The behavioral findings were further corroborated with estimation of neurotransmitters and their metabolites from mouse brain homogenate. Effect on learning and memory was evaluated by EPM, passive avoidance (PA) tests. Further, it was confirmed with assessment of acetylcholinesterase and caspase-3 activity in brain homogenate. EAMP showed significant anti-anxiety activity by increasing the time spent in open arm of EPM, light box of LDB. Social interaction time was increased significantly (p<0.01) as compared to vehicle control. There was also significant reduction of immobility time in both FST and TST without any changes in locomotor activity in the OFT. Monoamine neurotransmitters (dopamine and norepinephrine) concentrations were increased significantly (p<0.01) after 4 weeks of treatment as compared to stress control and substantiated the anti-depressant activity. Step down latency was increased (p<0.01) in PA test and transfer latency was decreased (p<0.01) in EPM test of EAMP-treated mice. Acetylcholinesterase and caspase-3 activity was significantly (p<0.05) changed in mice treated with EAMP (200 and 400 mg/kg). The results revealed that EAMP has anti-anxiety, anti-depressant and memory enhancing activities that are mediated through multiple mechanisms.

  19. Uptake and metabolic effects of salicylic acid on the pulvinar motor cells of Mimosa pudica L.

    Science.gov (United States)

    Dédaldéchamp, Fabienne; Saeedi, Saed; Fleurat-Lessard, Pierrette; Roblin, Gabriel

    2014-01-01

    In this paper, the salicylic acid (o-hydroxy benzoic acid) (SA) uptake by the pulvinar tissues of Mimosa pudica L. pulvini was shown to be strongly pH-dependent, increasing with acidity of the assay medium. This uptake was performed according to a unique affinity system (K(m) = 5.9 mM, V(m) = 526 pmol mgDW(-1)) in the concentration range of 0.1-5 mM. The uptake rate increased with increasing temperature (5-35 °C) and was inhibited following treatment with sodium azide (NaN3) and carbonyl cyanide m-chlorophenylhydrazone (CCCP), suggesting the involvement of an active component. Treatment with p-chloromercuribenzenesulfonic acid (PCMBS) did not modify the uptake, indicating that external thiol groups were not necessary. KCl, which induced membrane depolarization had no significant effect, and fusicoccin (FC), which hyperpolarized cell membrane, stimulated the uptake, suggesting that the pH component of the proton motive force was likely a driving force. These data suggest that the SA uptake by the pulvinar tissues may be driven by two components: an ion-trap mechanism playing a pivotal role and a putative carrier-mediated mechanism. Unlike other benzoic acid derivatives acting as classical respiration inhibitors (NaN3 and KCN), SA modified the pulvinar cell metabolism by increasing the respiration rate similar to CCCP and 2,4-dinitrophenol (DNP). Furthermore, SA inhibited the osmoregulated seismonastic reaction in a pH dependent manner and induced characteristic damage to the ultrastructural features of the pulvinar motor cells, particularly at the mitochondrial level. Copyright © 2013 Elsevier Masson SAS. All rights reserved.

  20. Development of an Agrobacterium-mediated stable transformation method for the sensitive plant Mimosa pudica.

    Directory of Open Access Journals (Sweden)

    Hiroaki Mano

    Full Text Available The sensitive plant Mimosa pudica has long attracted the interest of researchers due to its spectacular leaf movements in response to touch or other external stimuli. Although various aspects of this seismonastic movement have been elucidated by histological, physiological, biochemical, and behavioral approaches, the lack of reverse genetic tools has hampered the investigation of molecular mechanisms involved in these processes. To overcome this obstacle, we developed an efficient genetic transformation method for M. pudica mediated by Agrobacterium tumefaciens (Agrobacterium. We found that the cotyledonary node explant is suitable for Agrobacterium-mediated transformation because of its high frequency of shoot formation, which was most efficiently induced on medium containing 0.5 µg/ml of a synthetic cytokinin, 6-benzylaminopurine (BAP. Transformation efficiency of cotyledonary node cells was improved from almost 0 to 30.8 positive signals arising from the intron-sGFP reporter gene by using Agrobacterium carrying a super-binary vector pSB111 and stabilizing the pH of the co-cultivation medium with 2-(N-morpholinoethanesulfonic acid (MES buffer. Furthermore, treatment of the explants with the detergent Silwet L-77 prior to co-cultivation led to a two-fold increase in the number of transformed shoot buds. Rooting of the regenerated shoots was efficiently induced by cultivation on irrigated vermiculite. The entire procedure for generating transgenic plants achieved a transformation frequency of 18.8%, which is comparable to frequencies obtained for other recalcitrant legumes, such as soybean (Glycine max and pea (Pisum sativum. The transgene was stably integrated into the host genome and was inherited across generations, without affecting the seismonastic or nyctinastic movements of the plants. This transformation method thus provides an effective genetic tool for studying genes involved in M. pudica movements.

  1. Teratogenic effects of Mimosa tenuiflora in a rat model and possible role of N-methyl and N,N-dimethyltryptamine

    Science.gov (United States)

    Mimosa tenuiflora is a shrub/tree found in northeastern Brazil sometimes eaten by livestock and believed to be responsible for malformations observed in many animals from that region. The teratogenic compounds in M. tenuif lora are not known. This study used pregnant rats fed M. tenuif lora and comp...

  2. Pengaruh mimosa pada penyamakan kulit jaket domba samak nabati menggunakan sistem C-RFP, ditinjau dari sifat organoleptis, fisis, dan morfologi kulit

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    Sri Sutyasmi

    2016-06-01

    Full Text Available The purpose of this study was to determine the effect of the use of mimosa, to manufacture environmentally friendly leather for jacket by using the C-RFP system (C=Conditioning, R=Rapid, F=Fass (drum, P=Powder, on physical, organoleptic, and morphology properties of leather. It was also to obtain a formula for vegetable tanning with C-RFP system. Pickled skins are conditioned (pre-tanning using Sodotan TSN and Sodotan APR, and then Sodotan TSN was chosen due to it meets the requirements of SNI leather for jacket (SNI 4593:2011. Then, pickled skins were tanned with mimosa and applied C-RFP system or rapid tanning without water added. Mimosa, used in this research, were 15%, 20%, and 25% and fatliquor were 12.5%, 15%, and 17.5%. Furthermore, The leather were finished into an environmentally friendly leather for jackets, and then tested for physical and organoleptic properties based on the SNI 4593:2011 as well as leather morphology (SEM. The physical test result shows that for mimosa 15%, 20% and 25%, and for fatliquor 15% and 17.5% are fullfill the SNI.

  3. Phytochemical screening, antibacterial and free radical scavenging effects of Artemisia nilagirica, Mimosa pudica and Clerodendrum siphonanthus-An in-vitro study

    Institute of Scientific and Technical Information of China (English)

    Arokiyaraj S; Sripriya N; Bhagya R; Radhika B; Prameela L; Udayaprakash NK

    2012-01-01

    Objective: To evaluate methanolic extracts of leaves of Artemisia nilagirica, Mimosa pudica and Clerodendrum siphonanthus for phytochemical analysis, antibacterial activity and free radical scavenging activity. Methods: Antibacterial activity was performed by disc diffusion method against two gram positive and four gram negative strains. Free radical scavenging potential was evaluated using total antioxidant activity (thiocyanate method) and diphenyl-picryl-hydrazyl (DPPH) assay. Results: Results of the present study showed that Clerodendrum siphonanthus exhibited significant antibacterial effect against Klebsiella pneumoniae (30 mm), Proteus mirabilis (16 mm), Salmonella typhi (16 mm), Staphylococcus aureus (12 mm), Escherichia coli (11.5 mm) and Bacillus subtilis (10 mm). Mimosa pudica and Artemisia nilagirica showed good antibacterial effects. Clerodendrum siphonanthus was found to be extremely effective in scavenging lipid peroxide (IC50 8 mg/mL) and DPPH radicals (IC50 7 mg/mL), whereas Artemisia nilagirica andMimosa pudica showed moderate activity. Phytochemical analysis of these plants revealed presence of tannins, alkaloids, flavanoids, terpenoids and glycosides. Conclusions: This study showed that Artemisia nilagirica, Mimosa pudica and Clerodendrum siphonanthus may serve as a potential agent for new therapeutics.

  4. THE GENUS MIMOSA WITH SPECIAL REFERENCE TOM. QUADRIVALVIS L. VAR. LEPTOCARPA (D.C. EARNEDY, A NEW SPECIES RECORD FOR THE WEED FLORA IN MALAYSIA

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    BAKI, B. BAKAR

    1996-01-01

    Full Text Available An exploratory floristic survey of the genus Mimosa was conducted in 1993 to ascertain species diversity and their spatial patterns of distribution in Peninsular Malaysia. A new species record of uncertain indigene, M.. quadrivalvis was recorded for the first time in restricted localities along the roadsides in Pekan Darat and Bertam, Seberang Perai, Gurun and Bedong in Kedah in addition to widely distributed and seemingly ubiquitous presence of M. invisa Mart. ex. Colla and M. pudica L. The latter two species were mostly found in open, disturbed and derelict habitats, agricultural areas and ex-mining lands. Both species exhibited largely contagious and overdispersed distribution patterns with positive peaks in pattern intensity values although regularity or underdispersed distribution patterns do manifest in certain localities. The giant mimosa, M. pigra inhabited in clustered thickets, large pockets of lands in the urban and sub-urban localities in the states of Penang, Perak, Kelantan, Kuala Lumpur and Negeri Sembilan. In other states, M. pigra was confined to a few localities in smaller patches. Except for M. quadrivalvis, the other species of Mimosa are serious weeds in the agricultural, recreational and residential and derelict areas. A key to the Mimosa species is constructed along with brief descriptions on their morphology and ecology.

  5. Trypanocidal efficacy of two indigeneous ethanolic plant extracts (Mimosa pigra and Ipomoea asarifolia) against Trypanosoma evansi phospholipase A2 activity

    Institute of Scientific and Technical Information of China (English)

    Yusuf Alkali; A K Gana; Abdulkadir A; Nzelibe C Humphrey

    2015-01-01

    Objective:To study the inhibitory activity of ethanolic extract from Mimosa pigra and Ipomoea asarifolia against Trypanosoma evansi (T. evansi) calcium dependent phospholipase A2. Methods: The calcium dependent phospholipase A2 (E C 3.1.1.4) enzyme was isolated from T. evansi and purified to electrophoretic homogeneity under non denaturing conditions. It was solubilized from T. evansi cells recovered from white albino rats which were previously inoculated by intraperitoneal injection of infected camel blood. Two indigeneous ethanolic plant extracts used locally for treatment of trypanosomiasis were tested for the inhibition of phospholipases A2. Results: Double reciprocal plots of the initial velocity data of the inhibition by the indigenous plant extracts revealed a noncompetitive pattern of inhibition for the Ipomoea asarifolia and a competitive inhibition for Mimosa pigra in a dose dependent fashion. The extrapolated inhibition binding constant (Ki) of these extracts were found to be 2.0í102μg/mL and 1.12í102μg/mL respectively. Conclusions:The low Ki values obtained for these extracts towards this enzyme are an indication of high affinity of the extract or the active components (present in the plants) are for these enzyme and therefore, could be explored to serve as a cheap source of T. evansi PLA2 antidote and as well help in designing a novel drug with high efficiency.

  6. Cold plasma interactions with plants: Morphing and movements of Venus flytrap and Mimosa pudica induced by argon plasma jet.

    Science.gov (United States)

    Volkov, Alexander G; Xu, Kunning G; Kolobov, Vladimir I

    2017-12-01

    Low temperature (cold) plasma finds an increasing number of applications in biology, medicine and agriculture. In this paper, we report a new effect of plasma induced morphing and movements of Venus flytrap and Mimosa pudica. We have experimentally observed plasma activation of sensitive plant movements and morphing structures in these plants similar to stimulation of their mechanosensors in vivo. Application of an atmospheric pressure argon plasma jet to the inside or outside of a lobe, midrib, or cilia in Dionaea muscipula Ellis induces trap closing. Treatment of Mimosa pudica by plasma induces movements of pinnules and petioles similar to the effects of mechanical stimulation. We have conducted control experiments and simulations to illustrate that gas flow and UV radiation associated with plasma are not the primary reasons for the observed effects. Reactive oxygen and nitrogen species (RONS) produced by cold plasma in atmospheric air appear to be the primary reason of plasma-induced activation of phytoactuators in plants. Some of these RONS are known to be signaling molecules, which control plants' developmental processes. Understanding these mechanisms could promote plasma-based technology for plant developmental control and future use for plant protection from pathogens. Our work offers new insight into mechanisms which trigger plant morphing and movement. Copyright © 2017 Elsevier B.V. All rights reserved.

  7. Studies on the active components and antioxidant activities of the extracts of Mimosa pudica Linn. from southern China.

    Science.gov (United States)

    Zhang, Jing; Yuan, Ke; Zhou, Wen-Long; Zhou, Jian; Yang, Ping

    2011-01-01

    The total flavonoid (TF) and total phenolic (TP) contents of the ethanol extracts of the whole plant, stem, leaf, and seed of Mimosa pudica Linn belonging to the genus Mimosa (Family: Fabaceae alt. Leguminosae), which originates from the subtropical regions of southern China, were determined in this experiment. The antioxidant activity of the extracts and 5 flavonoid monomers of M. pudica Linn. were also evaluated by 2 assays, the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity and ferric reducing/antioxidant power (FRAP) assays. In addition, correlation analysis was also made in the present study. The results showed that leaf extracts contained the highest amount of TF and TP, and the content was significantly higher than that found in other parts of the plant. Moreover, the sequence of antioxidant activity of the ethanol extracts was as follows: leaf > the whole plant > seed > stem; the sequence of the 5 flavonoid monomers was as follows: 5,7,3´,4´-tetrahydroxy-6-C-[β-D-apiose-(1→4)]-β-D-glycopyranosyl flavone (1) > isorientin (2) > orientin (3) > isovitexin (4) > vitexin (5), and the antioxidant activity of compound 1 is equivalent to the synthetic antioxidant trolox or a bit stronger than trolox, and significant correlations were found among the active ingredient contents and the results of antioxidant activity. The present study suggested that M. pudica Linn. could be a potential rich source of natural antioxidants.

  8. [Description, distribution, anatomy, chemical composition and uses of Mimosa tenuiflora(Fabaceae-Mimosoideae) in Mexico].

    Science.gov (United States)

    Camargo-Ricalde, S L

    2000-12-01

    Because of some catastrophic events which occurred in Mexico during the 1980 decade, the utilization of "tepescohuite" bark against skin wounds and burns was popularized. The media manipulated the lack of available information about its medical properties and gave erroneous information to the society propagating a lot of myths. Therefore, the aim of this paper is to determine its taxonomic identity and to study the distribution, bark and wood anatomy of this species, and to determine its actual and historic uses, and the compilation of the information about bark pharmacology and toxicity. Its taxonomic identity is established as Mimosa tenuiflora (Willd.) Poir. (Fabaceae-Mimosoideae). It blooms and fructifies from November to June, occurring in Mexico (the states of Oaxaca and Chiapas), Guatemala, Honduras, El Salvador, Nicaragua, Panama, Colombia, Venezuela and Brazil, at altitudes of 0-1110 (-1520) m. In Mexico, it is found in dry forests, thorny thickets, Pinus and Pinus-Quercus forests, and in M. tenuiflora pure thickets, along roads and in resting or abandoned culture lands. This species has an aggregate distribution in the forests and a uniform one in the thickets. It presents a mean density of 9 individuals per m2, with 0.45 of frequency per quadrat and 1.69 m2 of mean coverture, and it has a wide range of tolerance to climatic and edaphic factors, confirming their invasive character. Regionally, the wood is used as fuel and fence construction, and against skin wounds and burns (bark tea, powder and/or ointment), and diverse products, such as shampoos, creams, capsules, soaps, etc., are commercialized. The bark is wrinkled, reddish-brown to grey, fibrous texture, 0.5-1.5 mm thick, resinous and with an astringent odor and flavor, and with a great quantity of tannins. The wood presents extremely short vessel elements, with alternate areolate punctuations, and simple perforated plates, vasicentric axial parenchima, confluent stripes, uniseriated rays, extremely

  9. EKSTRAKSI TANNIN SEBAGAI BAHAN PEWARNA ALAMI DARI TANAMAN PUTRIMALU (MIMOSA PUDICA MENGGUNAKAN PELARUT ORGANIK

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    Tjukup Marnoto

    2012-05-01

    Full Text Available EXTRACTION OF TANNINS AS NATURAL DYES FROM PUTRIMALU (MIMOSA PUDICA PLANT USING VARIOUS ORGANIC SOLVENTS. Public awareness on using natural dyes has encouraged the extraction of tannins from putrimalu. Extraction was performed continuously using a Soxhlet apparatus. The parameter studied was the influence of solvent polarity on the amount of tannin and mass transfer coefficient. Tannin was extracted from ten grams of dried putrimalu plants using polar solvents (ethanol, acetone and methanol and a non-polar solvent (n-hexane. Extraction is considered complete when the concentration of tannins in the liquid is no longer changing with time. Liquid samples were withdrawn every 20 minutes interval for tannin analyses using Thin Layer Chromatography (TLC. The results showed that the maximum concentration of tannins in the extract (g/mL when extraction were performed using n-hexane was 0.0031, acetone (0.016, methanol (0.0274 and ethanol (0.044. From extract yield and mass transfer coefficient point of views, it can be concluded that the best solvent is ethanol. The relationship between tannin concentration in the ethanol 96% solvent and the time was expressed in term of mathematical equations             CAL=1.046(1-e-0.0213t with error was 3.6%. Where CAL is the concentration of tannins in the solvent (g/ml and t is the extraction time (minutes.  Kesadaran masyarakat untuk kembali menggunakan bahan pewarna alami mendorong dilakukannya ekstraksi tannin dari putrimalu. Ekstraksi dilakukan secara kontinyu menggunakan alat Soxhlet. Parameter yang dipelajari adalah pengaruh polaritas pelarut terhadap ekstrak tannin dan koefisien transfer massa. Tannin diekstrak dari sepuluh gram tanaman putrimalu kering menggunakan pelarut polar (etanol, aseton dan metanol dan pelarut non-polar n-heksana. Ekstraksi dianggap selesai jika konsentrasi tannin di dalam pelarut sudah tidak berubah. Tannin di dalam contoh cairan yang diambil setiap selang waktu 20

  10. New Betaproteobacterial Rhizobium Strains Able To Efficiently Nodulate Parapiptadenia rigida (Benth.) Brenan

    Science.gov (United States)

    Taulé, Cecilia; Zabaleta, María; Mareque, Cintia; Platero, Raúl; Sanjurjo, Lucía; Sicardi, Margarita; Frioni, Lillian; Battistoni, Federico

    2012-01-01

    Among the leguminous trees native to Uruguay, Parapiptadenia rigida (Angico), a Mimosoideae legume, is one of the most promising species for agroforestry. Like many other legumes, it is able to establish symbiotic associations with rhizobia and belongs to the group known as nitrogen-fixing trees, which are major components of agroforestry systems. Information about rhizobial symbionts for this genus is scarce, and thus, the aim of this work was to identify and characterize rhizobia associated with P. rigida. A collection of Angico-nodulating isolates was obtained, and 47 isolates were selected for genetic studies. According to enterobacterial repetitive intergenic consensus PCR patterns and restriction fragment length polymorphism analysis of their nifH and 16S rRNA genes, the isolates could be grouped into seven genotypes, including the genera Burkholderia, Cupriavidus, and Rhizobium, among which the Burkholderia genotypes were the predominant group. Phylogenetic studies of nifH, nodA, and nodC sequences from the Burkholderia and the Cupriavidus isolates indicated a close relationship of these genes with those from betaproteobacterial rhizobia (beta-rhizobia) rather than from alphaproteobacterial rhizobia (alpha-rhizobia). In addition, nodulation assays with representative isolates showed that while the Cupriavidus isolates were able to effectively nodulate Mimosa pudica, the Burkholderia isolates produced white and ineffective nodules on this host. PMID:22226956

  11. Influência da temperatura de armazenamento na qualidade das sementes de Caesalpinia peltophoroides Benth. (sibipiruna

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    Pontes Claudia Aparecida

    2006-01-01

    Full Text Available Este trabalho teve como objetivo avaliar o efeito da temperatura de armazenamento na porcentagem de germinação e no vigor das sementes de Caesalpinia peltophoroides Benth. (sibipiruna, por meio de testes fisiológicos. O teor de água aumentou nas sementes armazenadas a 5 degreesC e reduziu naquelas a 20 degreesC. A porcentagem de germinação das sementes mantidas a 5 e 20 degreesC apresentou redução aos 220 e 120 dias, respectivamente. O índice de velocidade de germinação decresceu de maneira similar. A condutividade elétrica não foi alterada nas sementes armazenadas a 5 degreesC e aumentou nas que permaneceram a 20 degreesC. O envelhecimento acelerado nos períodos de 24, 48 e 72 horas detectou redução significativa na qualidade das sementes em ambos os ambientes de armazenamento. A redução da porcentagem de germinação e do vigor foi maior nas sementes armazenadas a 20 degreesC.

  12. (L. Benth. en bosques secundarios en Escárcega, Campeche, México

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    J. L. López-Torres

    2005-01-01

    Full Text Available El T´zalam Lysiloma latisiliquum (L. Benth. en la región de Escárcega, Campeche, México, es la especie más abundante y con mayor importancia económica en bosques secundarios derivados de selva mediana subperennifolia, después haber sido intervenida mediante el sistema rosa-tumba-quema. El objetivo de este trabajo es analizar el comportamiento del crecimiento en diámetro de esta especie, en un periodo de 20 años de desarrollo. A partir de la variable diámetro normal (d1.3, agrupada en categorías diamétricas, se estimaron los incrementos anuales medio, máximo y mínimo para cada categoría. Los resultados indican que en el escenario más ventajoso se requiere de 10.6 años para que los árboles pasen de categoría reserva a categoría aprovechable y, en promedio, es necesario de 21.5 años para alcanzar la misma condición. El incremento en diámetro fue, en promedio, de 0.511 cm y se clasifica como medio.

  13. In vitro anti- bacterial activity of leaves extracts of Albizia lebbeck Benth against some selected pathogens

    Institute of Scientific and Technical Information of China (English)

    Mohammed Nazneen Bobby; Edward Gnanaraj Wesely; MarimuthuAntonisamy Johnson

    2012-01-01

    Objective: To screen the anti-bacterial activity of Albizia lebbeck (A. lebbeck) Benth leaves extract against the selected bacterial pathogens viz., Bacillus subtilis (MTCC441), Escherichia coli (MTCC443), Klebsiella pneumonia (MTCC 109), Proteus vulgaris (MTCC742), Pseudomonas aeruginosa (MTCC741), Salmonella typhii (MTCC733) and Staphylococus aureus (MTCC96).Methods:The leaves extracts of A. lebbeck was tested against bacteria by the agar disc diffusion method. Results: Results of the present study indicated that different extracts of A. lebbeck showed inhibitory effects against the pathogens. The present study results demonstrated that methanolic extracts of A. lebbeck conferred the widest spectrum activities that inhibited the growth of all studied pathogens with the maximum zone of inhibition. The methanolic extracts ofA. lebbeck illustrated the highest zone of inhibition against the pathogens Bacillus subtilis (16 mm), Escherichia coli (22 mm), Klebsiella pneumonia (11 mm), Proteus vulgaris (18 mm), Pseudomonas aeruginosa (22 mm), Salmonella typhii (23 mm) and Staphylococus aureus (17 mm). The ethyl acetate extracts demonstrated maximum zone of inhibition against Escherichia coli (26 mm), Pseudomonas aeruginosa (22 mm) and Klebsiella pneumonia (16 mm). Conclusions: It is expected that this study would direct to the establishment of some active compounds that could be used to formulate new and more potent anti-bacterial drugs of natural origin.

  14. Seasonal variability of the essential oil of Hesperozygis ringens (Benth. Epling.

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    C. G. Pinheiro

    Full Text Available Abstract This study was developed to evaluate the effect of seasonality on the yield and chemical composition of the essential oil (EO of Hesperozygis ringens (Benth. Epling, a native species from the Brazilian Pampa. Leaves were collected from four specimens of a single population in each of the four seasons for a year and were extracted in triplicate by hydro-distillation for 2 hours. The yield of EO (% w/w was calculated on fresh weight basis (FWB, and the 16 oil samples were analyzed by gas chromatography coupled to mass spectrometry (GC-MS and gas chromatography with flame ionization detector (GC-FID. Hierarchical Cluster Analysis (HCA and Principal Component Analysis (PCA were used as statistical tools to evaluate differences in chemical composition. The highest yields were obtained in autumn, spring and summer (2.32-4.38%, while the lowest yields were detected in winter, ranging from 1.15 to 1.91%. Oxygenated monoterpenoids were the predominant class of chemical constituents in the EO obtained in all seasons, showing the highest contents in autumn and summer, and pulegone was identified as a major compound, whose contents varied between 54.13 and 81.17%. The EO samples were divided into three chemical groups by HCA and PCA and were assigned to the same group, except for the three samples gathered in winter. The results showed a seasonal influence on the yield and chemical composition of the EO.

  15. Chemical Variability and Biological Activities of Essential Oils of Micromeria inodora (Desf.) Benth. from Algeria.

    Science.gov (United States)

    Benomari, Fatima Zahra; Djabou, Nassim; Medbouhi, Ali; Khadir, Abdelmounaim; Bendahou, Mourad; Selles, Chaouki; Desjobert, Jean-Marie; Costa, Jean; Muselli, Alain

    2016-11-01

    The chemical composition of the essential oils isolated from the aerial parts of Micromeria inodora (Desf.) Benth. collected in 24 Algerian localities was investigated from the first time using GC-FID, GC/MS and (13) C-NMR. Altogether, 83 components which accounted for 94.7% of the total oil composition were identified. The main compounds were trans-sesquisabinene hydrate (1; 20.9%), α-terpinyl acetate (2; 19.8%), globulol (3; 4.9%), caryophyllene oxide (4; 4.3%), β-bisabolol (5; 2.9%) and trans-7-epi-sesquisabinene hydrate (6; 2.6%). Comparison with the literature highlighted the originality of the Algerian M. inodora oil and indicated that 1 might be used as taxonomical marker. The study of the chemical variability allowed the discrimination of two main clusters confirming that there is a relation between the essential-oil compositions and the soil nature of the harvest locations. Biological activity of M. inodora essential oil was assessed against fourteen species of microorganisms involved in nosocomial infections using paper disc diffusion and dilution agar assays. The in vitro study demonstrated a good activity against Gram-positive strains such as Staphylococcus aureus, Bacillus cereus, Bacillus subtilis, and Enterococcus faecalis, and moderate activity against Candida albicans. These results might be useful for the future commercial valorization of M. inodora essential oil as a promising source of natural products with potential against various nosocomial community and toxinic infections.

  16. Furano diterpenes from Pterodon pubescens Benth with selective in vitro anticancer activity for prostate cell line

    Energy Technology Data Exchange (ETDEWEB)

    Spindola, Humberto M.; Carvalho, Joao E. de; Ruiz, Ana Lucia T.G.; Rodrigues, Rodney A. F.; Denny, Carina; Sousa, Ilza M. de Oliveira; Foglio, Mary Ann [Universidade Estadual de Campinas (UNICAMP), SP (Brazil). Centro Pluridisciplinar de Pesquisas Quimicas, Biologicas e Agricolas (CPQBA)]. E-mail: foglioma@cpqba.unicamp.br; Tamashiro, Jorge Y. [Universidade Estadual de Campinas (UNICAMP), SP (Brazil). Inst. de Biologia

    2009-07-01

    Activity guided fractionation of Pterodon pubescens Benth. methylene chloride-soluble fraction afforded novel 6{alpha}-acetoxi 7{beta}-hydroxy-vouacapan 1 and four known diterpene furans 2, 3, 4, 5. The compounds were evaluated for in vitro cytotoxic activities against human normal cells and tumour cell lines UACC-62 (melanoma), MCF-7 (breast), NCI-H460 (lung, non-small cells), OVCAR-03 (ovarian), PC-3 (prostate), HT-29 (colon), 786-0 (renal), K562 (leukemia) and NCI-ADR/RES (ovarian expressing phenotype multiple drugs resistance). Results were expressed by three concentration dependent parameters GI{sub 50} (concentration that produces 50% growth inhibition), TGI (concentration that produces total growth inhibition or cytostatic effect) and LC{sub 50} (concentration that produces .50% growth, a cytotoxicity parameter). Also, in vitro cytotoxicity was evaluated against 3T3 cell line (mouse embryonic fibroblasts). Antiproliferative properties of compounds 1, 4 and 5 are herein reported for the first time. These compounds showed selectivity in a concentration-dependent way against human PC-3. Compound 1 demonstrated selectivity 26 fold more potent than the positive control, doxorubicin, for PC-3 (prostrate) cell line based on GI{sub 50} values, causing cytostatic effect (TGI value) at a concentration fifteen times less than positive control. Moreover comparison of 50% lethal concentration (LC{sub 50} value) with positive control (doxorubicin) suggested that compound 1 was less toxic. (author)

  17. PEMANFAATAN BAKTERI PELARUT FOSFAT UNTUK MENINGKATKAN PRODUKTIVITAS KUDZU TROPIKA (Pueraria phaseoloides Benth.

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    N. G. K Roni

    2014-06-01

    Full Text Available Phosphorus (P is one of the essential nutrients for plants that availability is strongly influenced by soil pH. In acid soils, most of the P is given will form insoluble compounds and is not available to plants. Phosphate solubilizing bacteria is a soil microorganism that can improve the provision ofP in acid soils. The study aimed to determine the effect of Phosphate solubilizing bacteria on the productivity of tropical kudzu was conducted using a completely randomized design with four treatments ie without isolate (I0, standard isolate (I1, isolate B.80.1649-1 (I2 and isolate B.80.1649-8 (I3, each treatment was repeated four times. The results showed that both isolates were isolated and selection were able to increase the length of the plants, number of leaves, shoot dry weight, root dry weight, shoot P content,  N uptake, P uptake, and production of crude protein of tropical kudzu (Pueraria phaseoloides Benth.. The ability of isolates B.80.1649-1 higher than isolates B.80.1649-8, and comparable with standard isolate.

  18. Chemical variability of Cleistopholis patens (Benth.) Engl. et Diels leaf oil from ivory coast.

    Science.gov (United States)

    Ouattara, Zana Adama; Boti, Jean Brice; Attioua, Koffi Barthelemy; Ahibo, Antoine Coffy; Casanova, Joseph; Tomi, Félix; Bighelli, Ange

    2013-11-01

    The chemical composition of 48 leaf oil samples isolated from individual plants of Cleistopholis patens (Benth.) Engl. et Diels harvested in four Ivoirian forests was investigated by GC-FID (determination of retention indices), GC/MS, and (13) C-NMR analyses. The main components identified were β-pinene (traces-59.1%), sabinene (traces-54.2%), (E)-β-caryophyllene (0.3-39.3%), linalool (0.1-38.5%), (E)-β-ocimene (0.1-33.2%), germacrene D (0.0-33.1%), α-pinene (0.1-32.3%), and germacrene B (0-21.2%). The 48 oil compositions were submitted to hierarchical clustering and principal components analyses, which allowed the distinction of three groups within the oil samples. The oil composition of the major group (GroupI, 33 samples) was dominated by (E)-β-caryophyllene and linalool. The oils of Group II (eight samples) contained mainly β-pinene and α-pinene, while those of Group III (seven samples) were dominated by sabinene, limonene, and β-phellandrene. Moreover, the compositions of the Ivoirian C. patens leaf oils differed from those of Nigerian and Cameroonian origins. Copyright © 2013 Verlag Helvetica Chimica Acta AG, Zürich.

  19. Phytochemical constituents, antioxidant activity and toxicity potential of Phlomis olivieri Benth.

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    M.R. Delnavazi

    2016-04-01

    Full Text Available Background and objectives: Phlomis olivieri Benth. (Lamiaceae is a medicinal plant widely distributed in Iran. In the present study, we have investigated the phytochemical constituents, antioxidant activity and general toxicity potential of the aerial parts of this species. Methods: Silica gel (normal and reversed phases and Sephadex LH-20 column chromatographies were used for isolation of compounds from methanol-soluble portion (MSP of the total extract obtained from P. olivieri aerial parts. The structures of isolated compounds were elucidated using 1H-NMR, 13C-NMR and UV spectral analyses. Antioxidant activity and general toxicity potential of MSP were also evaluated in DPPH free radical-scavenging assay and brine shrimp lethality test (BSLT, respectively. Results: One caffeoylquinic acid derivative, chlorogenic acid (1, one iridoid glycoside, ipolamiide (2, two phenylethanoid glycosides, phlinoside C (3 and verbascoside (5, along with two flavonoids, isoquercetin (4 and naringenin (6 were isolated and identified from MSP. The MSP exhibited considerable antioxidant activity in DPPH method (IC50; 50.4 ± 4.6 µg/mL, compared to BHT (IC50; 18.7 ± 2.1 µg/mL, without any toxic effect in BSLT at the highest tested dose (1000 µg/mL. Conclusion: the results of the present study introduce P. olivieri as a medicinal plant with valuable biological and pharmacological potentials.

  20. Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata Benth

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    A Della Torre

    2011-01-01

    Full Text Available Plathymenia reticulata Benth has an anti-inflammatory effect and is capable of neutralizing the neuromuscular blockade induced by Bothrops jararacussu or Crotalus durissus terrificus venoms, probably by precipitating venom proteins (an effect caused by plant tannins. The present study aimed to evaluate the mutagenic activity of P. reticulata by using the Salmonella mutagenicity assay (Ames test and the micronucleus test in CHO-K1 cells. P. reticulata extract concentrations of 2.84, 5.68, 11.37, and 19.90 mg/plate were assayed by the Ames test using TA97a, TA98, TA100 and TA102 bacterial strains, with (+S9 and without (-S9 metabolic activation. Concentrations of 5, 1.6 and 0.5 μg/mL of P. reticulata extract were used for the micronucleus test. P. reticulata extract was mutagenic to TA98 (-S9 and showed signs of mutagenic activity in TA97a and TA102 (both -S9 strains. Micronucleus test CBPI values showed that the endogenous metabolic system increased the number of viable cells when compared to the non-activated samples and the micronucleus frequency increased when the cells were treated in the absence of S9. We concluded that P. reticulata extract may present direct mutagenic properties.

  1. Emergência e desenvolvimento inicial de sementes de Dimorphandra mollis Benth. em campo

    Directory of Open Access Journals (Sweden)

    M.F. SOUZA

    2016-03-01

    Full Text Available RESUMO Dimorphandra mollis Benth. (Leguminosae-Caesalpinioideae é uma espécie arbórea, encontrada no bioma Cerrado, utilizada na recuperação de áreas degradadas e na ornamentação. Dos frutos, extrai-se a rutina, um bioflavonoide utilizado na indústria farmacêutica, o qual atua na permeabilidade e na resistência dos vasos capilares. Esta pesquisa teve como objetivo verificar os efeitos do tipo de solo e de adubos sobre a emergência e o vigor da fava-d’anta semeada diretamente no campo. O delineamento experimental adotado foi em blocos ao acaso, em esquema fatorial 4 x 2 (quatro tipos de adubação e dois tipos de solo, com sete repetições de cinco sementes cada. Foram avaliadas as seguintes características: emergência, primeira contagem da emergência, índice de velocidade de emergência, altura e diâmetro da plântula. Não houve interação significativa entre os fatores, tipo de solo e adubos. Os melhores resultados para emergência e IVE são obtidos quando a semeadura é realizada em solo local.

  2. Flavonoids with acetylated branched glycans and bioactivity of Tipuana tipu (Benth.) Kuntze leaf extract.

    Science.gov (United States)

    Afifi, Manal S; Elgindi, Omaima D; Bakr, Reham O

    2014-01-01

    The new acetylated kaempferol tetraglycoside, kaempferol-3-O-[2″(4-acetylrhamnopyranosyl)-3″-galactopyranosyl] robinobioside (1), was isolated from the aqueous methanolic leaf extract of Tipuana tipu Benth. The known kaempferol 3-[2″-(4-acetyl-rhamnosyl)] robinobioside (2), kaempferol 3-O-2″-rhamnopyranosylrutinoside (3), rutin (4), kaempferol 3-O-rutinoside (5), kaempferol 3-O-glucopyranoside (6), kaempferol 3-O-galactopyranoside (7), quarcetin 3-O-glucopyranoside (8), kaempferol (9) and quercetin (10) together with the chlorogenic acid (11) were also isolated and characterised. Structures were established on the basis of chemical and spectroscopic analysis including (1)H NMR, (13)C NMR, 2D NMR and ESI-MS. The methanol extract exhibited moderate antioxidant activity, IC50 28.96 μg/mL, compared with ascorbic acid (1.83 μg/mL) and tertiary-butylhydroquinone (1.92 μg/mL). The methanol and chloroform extracts exhibited potent cytotoxic activity; the former was found to be active against larynx and liver cell lines, while the latter being active against intestine and liver cell lines.

  3. Chemical investigation of the medicinal and ornamental plant Angelonia angustifolia Benth. reveals therapeutic quantities of lupeol.

    Science.gov (United States)

    Deyrup, Stephen T; Asghar, Khush B; Chacko, Ann; Hebert, Jakob M; Samson, Eric; Talone, Christopher J

    2014-10-01

    Angelonia angustifolia Benth. is a small herbaceous plant with documented use as an anti-inflammatory remedy by indigenous cultures in Latin America. It has subsequently been developed as an ornamental annual widely available in nurseries in the United States. Chemical investigation led to the discovery that lupeol is the major organic soluble constituent in the roots, and is present in large quantities in the aerial structures of the plant. Lupeol was identified by 1D and 2D NMR spectroscopic techniques and quantified by HPLC-MS. The concentration of lupeol (9.14 mg/g in roots) in A. angustifolia is approximately 3 times higher than any previously reported sources. Therefore, the amount of lupeol in the roots of a single individual of A. angustifolia greatly exceeds the previously determined topical threshold for significant reduction of inflammation. The presence of topically therapeutic levels of lupeol in A. angustifolia provides chemical rationale for its indigenous use. In addition, the established cultivation of A. angustifolia could allow this plant to be used as a source of the important bioactive molecule lupeol, or to be developed as a nutraceutical without damaging wild populations.

  4. Color, ellagitannins, anthocyanins, and antioxidant activity of Andean blackberry (Rubus glaucus Benth.) wines.

    Science.gov (United States)

    Arozarena, Íñigo; Ortiz, Jacqueline; Hermosín-Gutiérrez, Isidro; Urretavizcaya, Inés; Salvatierra, Sara; Córdova, Inés; Marín-Arroyo, María Remedios; Noriega, María José; Navarro, Montserrat

    2012-08-01

    Twenty-eight blackberry ( Rubus glaucus Benth.) wines elaborated under different processing conditions were analyzed for total phenolics, ellagitannins, anthocyanins, color, and antioxidant activity. Ellagitannins were the main phenolic compounds and the most determinant factor in the antioxidant capacity of wines (r = 0.980). The major anthocyanins were cyanidin 3-rutinoside (64 ± 6%) and cyanidin 3-glucoside (19 ± 4%), followed by several minor compounds (17 ± 4%). Two of them were native blackberry anthocyanins, namely, cyanidin 3-rutinoside-5-glucoside and cyanidin 3-xylorutinoside. The remaining seven compounds were anthocyanin-related pigments generated during and after the alcoholic fermentation, identified as A-type and B-type vitisins and hydroxyphenylpyranoanthocyanins. The presence of fruit solids in contact with the liquid fraction during fermentation and the ratio of water to fruit employed in the preparation of the musts had a great impact on the content of ellagitannins, total phenolics, and the antioxidant activity of wines and a minor impact on their color and anthocyanin composition.

  5. DORMÊNCIA DE SEMENTES E PRODUÇÃO DE MUDAS DE Dimorphandra mollis Benth.

    Directory of Open Access Journals (Sweden)

    Mauro Vasconcelos Pacheco

    2011-01-01

    Full Text Available Dimorphandra mollis Benth. is an important native forest species coming from Cerrado and Caatinga due to its economical and ecological use, what justifies the existence of programs of seedling production. This paper aimed to study the efficient methodology to overcome dormancy in seeds and to evaluate, in natural nursery, the effects of distinct substrates and the fertilization on the initial growth of plants. The following treatments applied: T1 - control (seeds whithout scarification; T2 ¿ manual scarification with sandpaper number 50; T3 - immersion in water at 80 and (T4 at 100ºC; T5 - boil in water at 100ºC for 10, (T6 30 and (T7 60 seconds; T8 - chemical scarification with sulfuric acid for 20, (T9 30 and (T10 40 minutes. For the seedling production, the experiment was arranged in 4 x 2 factorial, complete randomized design (4 substrates: Tropstrato® pure, and the others: Tropstrato®, coconut fiber and vermiculite with organic compost; without and with fertilization. The best results for overcoming dormancy are obtained with the manual scarification with sandpaper nº50 or boil in water at 100ºC for 10 seconds. The coconut fiber and the vermiculite with organic compost are good substrates for the seedling production of Dimorphandra mollis.

  6. Fatty acid profile of gamma-irradiated and cooked African oil bean seed (Pentaclethra macrophylla Benth).

    Science.gov (United States)

    Olotu, Ifeoluwa; Enujiugha, Victor; Obadina, Adewale; Owolabi, Kikelomo

    2014-11-01

    The safety and shelf-life of food products can be, respectively, ensured and extended with important food-processing technologies such as irradiation. The joint effect of cooking and 10 kGy gamma irradiation on the fatty acid composition of the oil of Pentaclethra macrophylla Benth was evaluated. Oils from the raw seed, cooked seeds, irradiated seeds (10 kGy), cooked, and irradiated seeds (10 kGy) were extracted and analyzed for their fatty acid content. An omega-6-fatty acid (linoleic acid) was the principal unsaturated fatty acid in the bean seed oil (24.6%). Cooking significantly (P oil sample to have the highest total fatty acid content (154.9%), unsaturated to saturated fatty acid ratio (109.6), and unsaturated fatty acid content (153.9%). 10 kGy irradiation induces the formation of C20:5 (eicosapentaenoic), while cooking induced the formation of C20:4 (arachidic acid), C22:6 (Heneicosanoic acid), and C22:2 (docosadienoic acid). Combined 10 kGy cooking and irradiation increased the susceptibility of the oil of the African oil bean to rancidity.

  7. Preparation and characterization of microcapsules of Pterodon pubescens Benth. by using natural polymers

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    Alexandre Espada Reinas

    2014-12-01

    Full Text Available An oleaginous fraction obtained from an alcohol extract of the fruit of Pterodon pubescensBenth. (FHPp was microencapsulated in polymeric systems. These systems were developed using a complex coacervation method and consisted of alginate/medium-molecular-weight chitosan (F1-MC, alginate/chitosan with greater than 75% deacetylation (F2-MC, and alginate/low-molecular-weight chitosan (F3-MC. These developed systems have the potential to both mask the taste of the extract, and to protect its constituents against possible chemical degradation. The influence of the formulation parameters and process were determined by chemical profiling and measurement of the microencapsulation efficiency of the oleaginous fraction, and by assessment of microcapsule morphology. The obtained formulations were slightly yellow, odorless, and had a pleasant taste. The average diameters of the microcapsules were 0.4679 µm (F2-MC, 0.5885 µm (F3-MC, and 0.9033 µm (F1-MC. The best formulation was F3-MC, with FHPp microencapsulation efficiency of 61.01 ± 2.00% and an in vitro release profile of 75.88 ± 0.45%; the content of vouacapans 3-4 was 99.49 ± 2.80%. The best model to describe the release kinetics for F1-MC and F3-MC was that proposed by Higuchi; however, F2-MC release displayed first-order kinetics; the release mechanism was of the supercase II type for all formulations.

  8. Regulatory roles of 24-epibrassinolide in tolerance of Acacia gerrardii Benth to salt stress.

    Science.gov (United States)

    Abd Allah, Elsayed Fathi; Alqarawi, A A; Hashem, Abeer; Wirth, Stephan; Egamberdieva, Dilfuza

    2017-07-11

    This experiment aimed to investigate the role of 24-epibrassinolide (EBL) against NaCl-induced salinity stress in Acacia gerrardii Benth. NaCl (200 mM) imparted deleterious effects on the growth and chlorophyll contents of A. gerrardii, but foliar application of EBL (1.0 mg/l; each plant received 2.5 ml) mitigated the negative effect considerably. NaCl reduced chlorophyll content but this was significantly ameliorated by the application of EBL. EBL reduced significantly NaCl-induced oxidative stress hence protect membranes and also improved the relative water content significantly by 6.6% as compared with control. Nitrate reductase activity declined after NaCl treatment but EBL application sustained its activity under normal and stressed conditions. Exogenous application of EBL significantly improved the activity of superoxide dismutase, catalase and the enzymes of the ascorbate-glutathione pathway thereby protecting the photosynthetic electron transport chain and other metabolic processes in A. gerrardii from NaCl-induced oxidative stress.

  9. Evaluation of the immunotoxicological effects of Dimorphandra mollis Benth., Fabaceae, in rats Avaliação dos efetos imunotoxicológicos de Dimorphandra mollis Benth., Fabaceae, em ratos

    OpenAIRE

    2010-01-01

    Dimorphandra mollis Benth., Fabaceae, also known as "faveira" or "fava-d'anta", is a plant common to the central woodsy meadow region of Brazil. It is well known for its antioxidant, antiplatelet and, principally, vasoprotective properties. Its principal component is rutin. The objective of this study is the evaluation of the safety of the use of the dried D. mollis extract in rodents. The rutin content of the standardized extract was 76.0±3%. With respect to the biochemical and hematological...

  10. Antimicrobial activity of Mimosa tenuiflora (Willd. Poir. from Northeast Brazil against clinical isolates of Staphylococcus aureus Atividade antimicrobiana de Mimosa tenuiflora (Willd. Poir. do Nordeste do Brasil, contra isolados clínicos de Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Itácio Q. M. Padilha

    2010-03-01

    Full Text Available Mimosa tenuiflora is a native plant of Northeast Brazil where it is popularly known as ''jurema-preta'' and it is widely used in folk medicine. In this work the anti-Staphylococcus aureus activity of ethanol extract of M tenuiflora was evaluated by determination of minimum inhibitory concentration (MIC of clinical isolates by the agar dilution method, and by time-kill assay using a reference strain. MIC values against 30 isolates were 0,18 mg/mL (16/30 or 0,36 mg/mL (14/30, and also the reference strain. In the reference strains, at concentrations up to 4x MIC, only bacteriostatic effect was observed, but at 8x MIC a fast bactericidal effect was observed.A Mimosa tenuiflora é uma planta nativa da região Nordeste do Brasil onde é conhecida como jurema-preta sendo amplamente utilizada na medicina popular. No presente trabalho a atividade anti-Staphylococcus aureus do extrato etanólico da M. tenuiflora foi avaliada pela determinação da concentração inibitória mínima (CIM, pelo método da diluição em agar, em 30 isolados clínicos e pela cinética de inativação com a linhagem referência. Os valores da CIM foram 0,18 mg/mL em 16 isolados e 0,36 mg/mL nos demais, bem como na linhagem referência. A cinética de inativação mostrou apenas efeito bacteriostático nas concentrações do extrato até aquela correspondente a 4x CIM e um rápido efeito bactericida na concentração correspondente a 8x CIM.

  11. Effect of different liming levels on the biomass production and essential oil extraction yield of Cunila galioides Benth

    OpenAIRE

    2012-01-01

    Poejo is an aromatic and medicinal plant native to highland areas of south Brazil, in acid soils with high Al3+ concentration. The main objective of the present work was to evaluate the effect of liming on the extraction yield of essential oil of three chemotypes of poejo (Cunila galioides Benth). For this purpose, the experiments were performed in a greenhouse, using 8-litre pots. The treatments were four dosages of limestone (0, 3.15, 12.5, and 25 g.L-1) and a completely random experimental...

  12. Induction of nodD Gene in a Betarhizobium Isolate, Cupriavidus sp. of Mimosa pudica, by Root Nodule Phenolic Acids.

    Science.gov (United States)

    Mandal, Santi M; Chakraborty, Dipjyoti; Dutta, Suhrid R; Ghosh, Ananta K; Pati, Bikas R; Korpole, Suresh; Paul, Debarati

    2016-06-01

    A range of phenolic acids, viz., p-coumaric acid, 4-hydroxybenzaldehyde, 4-hydroxybenzoic acid, protocatechuic acid, caffeic acid, ferulic acid, and cinnamic acid have been isolated and identified by LC-MS analysis in the roots and root nodules of Mimosa pudica. The effects of identified phenolic acids on the regulation of nodulation (nod) genes have been evaluated in a betarhizobium isolate of M. pudica root nodule. Protocatechuic acid and p-hydroxybenzoic acid were most effective in inducing nod gene, whereas caffeic acid had no significant effect. Phenylalanine ammonia lyase, peroxidase, and polyphenol oxidase activities were estimated, indicating regulation and metabolism of phenolic acids in root nodules. These results showed that nodD gene expression of betarhizobium is regulated by simple phenolic acids such as protocatechuic acid and p-hydroxybenzoic acid present in host root nodule and sustains nodule organogenesis.

  13. In vitro and In vivo Antioxidant evaluation and estimation of total phenolic, flavonoidal content of Mimosa pudica L

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    Ganesh Patro

    2016-01-01

    Full Text Available Objective: Mimosa pudica Linn. (Mimosaceae is traditionally used as a folk medicine to treat various ailments including convulsions, alopecia, diarrhea, dysentery, insomnia, tumor, wound healing, snake bite, etc., Here, the study was aimed to evaluate the antioxidant potential of M. pudica leaves extract against 2, 2-diphenyl-1-picrylhydrazyl (DPPH (in vitro and its modulatory effect on rat brain enzymes. Materials and Methods: Total phenolic, flavonoid contents, and in vitro antioxidant potential against DPPH radical were evaluated from various extracts of M. pudica leaves. In addition, ethyl acetate extract of Mimosa pudica leaves (EAMP in doses of 100, 200, and 400 mg/kg/day were administered orally for 7 consecutive days to albino rats and evaluated for the oxidative stress markers as thiobarbituric acid reactive substances (TBARS, superoxide dismutase (SOD, catalase (CAT, and glutathione (GSH from rat brain homogenate. Results: The ethyl acetate extract showed the highest total phenolic content and total flavonoid content among other extracts of M. pudica leaves. The percentage inhibition and IC 50 value of all the extracts were followed dose-dependency and found significant (P < 0.01 as compared to standard (ascorbic acid. The oxidative stress markers as SOD, CAT, and GSH were increased significantly (P < 0.01 at 200 and 400 mg/kg of EAMP treated animals and decreased significantly the TBARS level at 400 mg/kg of EAMP as compared to control group. Conclusion: These results revealed that the ethyl acetate extract of M. pudica exhibits both in vitro antioxidant activity against DPPH and in vivo antioxidant activity by modulating brain enzymes in the rat. This could be further correlated with its potential to neuroprotective activity due to the presence of flavonoids and phenolic contents in the extract.

  14. In vitro and In vivo Antioxidant Evaluation and Estimation of Total Phenolic, Flavonoidal Content of Mimosa pudica L

    Science.gov (United States)

    Patro, Ganesh; Bhattamisra, Subrat Kumar; Mohanty, Bijay Kumar; Sahoo, Himanshu Bhusan

    2016-01-01

    Objective: Mimosa pudica Linn. (Mimosaceae) is traditionally used as a folk medicine to treat various ailments including convulsions, alopecia, diarrhea, dysentery, insomnia, tumor, wound healing, snake bite, etc., Here, the study was aimed to evaluate the antioxidant potential of M. pudica leaves extract against 2, 2-diphenyl-1-picrylhydrazyl (DPPH) (in vitro) and its modulatory effect on rat brain enzymes. Materials and Methods: Total phenolic, flavonoid contents, and in vitro antioxidant potential against DPPH radical were evaluated from various extracts of M. pudica leaves. In addition, ethyl acetate extract of Mimosa pudica leaves (EAMP) in doses of 100, 200, and 400 mg/kg/day were administered orally for 7 consecutive days to albino rats and evaluated for the oxidative stress markers as thiobarbituric acid reactive substances (TBARS), superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) from rat brain homogenate. Results: The ethyl acetate extract showed the highest total phenolic content and total flavonoid content among other extracts of M. pudica leaves. The percentage inhibition and IC50 value of all the extracts were followed dose-dependency and found significant (P pudica exhibits both in vitro antioxidant activity against DPPH and in vivo antioxidant activity by modulating brain enzymes in the rat. This could be further correlated with its potential to neuroprotective activity due to the presence of flavonoids and phenolic contents in the extract. SUMMARY Total phenolic, flavonoid contents and in-vitro antioxidant potential were evaluated from various extracts of M. pudica leaves. Again, in-vivo antioxidant evaluation from brain homogenate on oxidative stress markers as TBARS, SOD, CAT and GSH from rat was investigated. Our findings revealed that M. pudica possesses both in-vitro and in-vivo antioxidant activity due to presence of phenolics and flavonoids. PMID:26941532

  15. Nutritional benefits of Crematogaster mimosae ants and Acacia drepanolobium gum for patas monkeys and vervets in Laikipia, Kenya.

    Science.gov (United States)

    Isbell, Lynne A; Rothman, Jessica M; Young, Peter J; Rudolph, Kathleen

    2013-02-01

    Patas monkeys (Erythrocebus patas) are midsized primates that feed extensively on the gum of Acacia drepanolobium and the ants are housed in swollen thorns of this Acacia. Their diet resembles that expected more of smaller bodied primates. Patas monkeys are also more like smaller bodied primates in reproducing at high rates. We sought to better understand the convergence of patas monkeys with smaller bodied primates by comparing their feeding behavior on ants and gum with that of closely related, sympatric vervets (Chlorocebus pygerythrus), and analyzing the nutrient content of the gum of A. drepanolobium and of Crematogaster mimosae, the most common ant species eaten by patas monkeys in Laikipia, Kenya. All occurrences of feeding and moving during focal animal sampling revealed that 1) patas monkeys seek A. drepanolobium gum but vervets avoid it; 2) both species open swollen thorns most often in the morning when antsare less active; 3) patas monkeys continually feed onswollen thorns and gum while moving quickly throughout the day, whereas vervets reduce their consumption of these items and their travel rate at mid-day, and; 4) vervets eat young swollen thorns at a higher rate than patas monkeys. Patas monkeys are able to spend little time acquiring substantial amounts of energy, protein, and minerals from A. drepanolobium gum and C. mimosae ants each day. These findings, when coupled with evidence of causes of infant and adult female mortality, suggest that reproductive success of female patas monkeys is more immediately affected by illness, disease, interactions between adults and infants, and access to water than by food.

  16. Biosynthesis of branched-chain amino acids is essential for effective symbioses between betarhizobia and Mimosa pudica.

    Science.gov (United States)

    Chen, Wen-Ming; Prell, Jurgen; James, Euan K; Sheu, Der-Shyan; Sheu, Shih-Yi

    2012-07-01

    Burkholderia phymatum STM815 and Cupriavidus taiwanensis LMG19424 are betaproteobacterial strains that can effectively nodulate several species of the large legume genus Mimosa. A Tn5 mutant, derived from B. phymatum STM815 (KM60), and another derived from C. taiwanensis LMG19424 (KM184-55) induced Fix(-) nodules on Mimosa pudica. The Tn5-interrupted genes of the mutants showed strong homologies to ilvE, which encodes a branched-chain amino acid aminotransferase, and leuC, which encodes the large subunit of isopropylmalate isomerase. Both enzymes are known to be involved in the biosynthetic pathways for branched-chain amino acids (BCAAs) (leucine, valine and isoleucine). The B. phymatum ilvE mutant, KM60, was not auxotrophic for BCAAs and could grow well on minimal medium with pyruvate as a carbon source and ammonia as a nitrogen source. However, it grew less efficiently than the wild-type (WT) strain when ammonia was substituted with valine or isoleucine as a nitrogen source. The BCAA aminotransferase activity of KM60 was significantly reduced relative to the WT strain, especially with isoleucine and valine as amino group donors. The C. taiwanensis leuC mutant, KM184-55, could not grow on a minimal medium with pyruvate as a carbon source and ammonia as a nitrogen source, but its growth was restored when leucine was added to the medium. The isopropylmalate isomerase activity of KM184-55 was completely lost compared with the WT strain. Both mutants recovered their respective enzyme activities after complementation with the WT ilvE or leuC genes and were subsequently able to grow as well as their parental strains on minimal medium. They were also able to form nitrogen-fixing nodules on M. pudica. We conclude that the biosynthesis of BCAAs is essential for the free-living growth of betarhizobia, as well as for their ability to form effective symbioses with their host plant.

  17. Rhizobium altiplani sp. nov., isolated from effective nodules on Mimosa pudica growing in untypically alkaline soil in central Brazil.

    Science.gov (United States)

    Baraúna, Alexandre C; Rouws, Luc F M; Simoes-Araujo, Jean L; Dos Reis Junior, Fábio B; Iannetta, Pietro P M; Maluk, Marta; Goi, Silvia R; Reis, Veronica M; James, Euan K; Zilli, Jerri E

    2016-10-01

    Root nodule bacteria were isolated from nodules on Mimosa pudica L. growing in neutral-alkaline soils from the Distrito Federal in central Brazil. The 16S rRNA gene sequence analysis of 10 strains placed them into the genus Rhizobium with the closest neighbouring species (each with 99 % similarity) being Rhizobium grahamii, Rhizobium cauense, Rhizobium mesoamericanum and Rhizobium tibeticum. This high similarity, however, was not confirmed by multi-locus sequence analysis (MLSA) using three housekeeping genes (recA, glnII and rpoB), which revealed R. mesoamericanum CCGE 501T to be the closest type strain (92 % sequence similarity or less). Chemotaxonomic data, including fatty acid profiles [with majority being C19 : 0 cyclo ω8c and summed feature 8 (C18 : 1ω7c/C18 : 1ω6c)], DNA G+C content (57.6 mol%), and carbon compound utilization patterns supported the placement of the novel strains in the genus Rhizobium. Results of average nucleotide identity (ANI) differentiated the novel strains from the closest species of the genus Rhizobium, R. mesoamericanum, R. grahamii and R. tibeticum with 89.0, 88.1 and 87.8 % similarity, respectively. The symbiotic genes essential for nodulation (nodC) and nitrogen fixation (nifH) were most similar (99-100 %) to those of R. mesoamericanum, another Mimosa-nodulating species. Based on the current data, these 10 strains represent a novel species of the genus Rhizobium for which the name Rhizobium altiplani sp. nov. is proposed. The type strain is BR 10423T (=HAMBI 3664T).

  18. In vitro and In vivo Antioxidant Evaluation and Estimation of Total Phenolic, Flavonoidal Content of Mimosa pudica L.

    Science.gov (United States)

    Patro, Ganesh; Bhattamisra, Subrat Kumar; Mohanty, Bijay Kumar; Sahoo, Himanshu Bhusan

    2016-01-01

    Mimosa pudica Linn. (Mimosaceae) is traditionally used as a folk medicine to treat various ailments including convulsions, alopecia, diarrhea, dysentery, insomnia, tumor, wound healing, snake bite, etc., Here, the study was aimed to evaluate the antioxidant potential of M. pudica leaves extract against 2, 2-diphenyl-1-picrylhydrazyl (DPPH) (in vitro) and its modulatory effect on rat brain enzymes. Total phenolic, flavonoid contents, and in vitro antioxidant potential against DPPH radical were evaluated from various extracts of M. pudica leaves. In addition, ethyl acetate extract of Mimosa pudica leaves (EAMP) in doses of 100, 200, and 400 mg/kg/day were administered orally for 7 consecutive days to albino rats and evaluated for the oxidative stress markers as thiobarbituric acid reactive substances (TBARS), superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) from rat brain homogenate. The ethyl acetate extract showed the highest total phenolic content and total flavonoid content among other extracts of M. pudica leaves. The percentage inhibition and IC50 value of all the extracts were followed dose-dependency and found significant (P pudica exhibits both in vitro antioxidant activity against DPPH and in vivo antioxidant activity by modulating brain enzymes in the rat. This could be further correlated with its potential to neuroprotective activity due to the presence of flavonoids and phenolic contents in the extract. Total phenolic, flavonoid contents and in-vitro antioxidant potential were evaluated from various extracts of M. pudica leaves. Again, in-vivo antioxidant evaluation from brain homogenate on oxidative stress markers as TBARS, SOD, CAT and GSH from rat was investigated. Our findings revealed that M. pudica possesses both in-vitro and in-vivo antioxidant activity due to presence of phenolics and flavonoids.

  19. Estudo das folhas e caule de Hyptidendron canum(Pohl ex Benth. Harley, Lamiaceae

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    Tatiana S. Fiuza

    2010-05-01

    Full Text Available Hyptidendron canum (Pohl ex Benth. Harley, Lamiaceae, é utilizada popularmente como antimalárica, antiinflamatória, antiulcerativa, anti-hepatotóxica e anticancerígena. O objetivo deste trabalho foi realizar o estudo morfo-anatômico das folhas e caules e identificar as principais classes de metabólitos secundários presentes nas folhas de H. canum, dados ainda não descritos na literatura. As folhas e caules jovens coletados em Goiânia (GO foram seccionados à mão livre e preparados para análise microscópica. Foram realizadas reações de identificação de metabólitos secundários do material dessecado e pulverizado. Preparou-se o extrato etanólico bruto, que posteriormente foi fracionado por partição líquido-líquido com hexano, clorofórmio e acetato de etila. As frações foram submetidas à análise cromatográfica em camada delgada (CCD. As lâminas foliares apresentam epiderme adaxial constituída por células poligonais com parede reta. Na epiderme abaxial observam-se células com parede reta a ondulada e estômatos diacíticos e anisocíticos. Tricomas tectores e glandulares estão presente em ambas as faces da lâmina foliar. O pecíolo apresenta aspecto canaletado, epiderme adaxial e abaxial unisseriada. O caule, em secção transversal possui contorno em geral quadrangular, com presença de tricomas tectores e glandulares. As reações e a CCD das folhas evidenciaram a presença de flavonóides, saponinas, terpenos e lignanas. Este trabalho contribuiu para um maior conhecimento da morfo-anatomia e das classes químicas presentes em H. canum.Hyptidendron canum(Pohl ex Benth. Harley, Lamiaceae, is popularly used as an antimalarial, anti-inflammatory, antiulcerative, antihepatotoxic and anticancer agent. The goal of this research was to perform the morphoanatomy study of H. canumleaves and stem and identify the main classes of secondary metabolites present in the of H. canumleaves. Such data have not been reported in the

  20. PRELIMINARY STUDY OF THE EXTRACT OF THE BARKS OF Licania macrophylla BENTH: PHYTOCHEMICALS AND TOXICOLOGICAL ASPECTS

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    Ryan da Silva Ramos

    2014-04-01

    Full Text Available O objetivo desta pesquisa foi avaliar o perfil fitoquímico, físico-químico da espécie vegetal e determinar a toxicidade do extrato bruto etanólico de L. macrophylla Benth frente às larvas de Artemia salina L. A análise fitoquímica foi realizado por meio do extrato bruto etanólico das cascas do caule e a determinação físico-química foi realizada de acordo com a Farmacopeia Brasileira. As análises fitoquímicas detectaram a presença de saponinas, ácidos orgânicos, açúcares redutores, taninos, antraquinonas, depsídios e depsidonas. Em relação aos parâmetros físico-químicos a planta apresentou pH=4,64, pela ocorrência de saponinas, ácidos orgânicos e taninos; Lipídeos= 0,55% indica que o material vegetal apresenta poucos lipídios de baixo peso molecular; Umidade= 12,09%±0,12, o que relaciona a pouca quantidade de água, fator indispensável para a não ocorrência de desenvolvimento de microrganismo ou degradação enzimática. Os resíduos por incineração (cinzas da espécie se encontra dentro dos padrões farmacognósticos de 8,30%±0,54. O extrato bruto segundo o teste de toxicidade é atóxico com CL50=1253µg/mL, isto é, confirma-se a relação estabelecida entre a taxa de mortalidade e CL50 deve ser superior a 1000µg/mL para serem considerados atóxicos. As análises fitoquímicas confirmaram em parte a utilização da espécie para fins fitoterápicos, porém a forma de tratamento e acondicionamento pode influenciar na determinação de metabólitos secundários. Os parâmetros físico-químicos adotados mostraram que espécie encontra-se livre de agentes decompositores. Palavras-chave: Fitoquímica, L. macrophilla Benth, Toxicidade, Físico-Química. DOI: http://dx.doi.org/10.18561/2179-5746/biotaamazonia.v4n1p94-99

  1. Gastroprotective effects of the essential oil of Hyptis crenata Pohl ex Benth. on gastric ulcer models.

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    Diniz, Lúcio Ricardo Leite; Vieira, Charliene Freire Xavier; Santos, Edgleyson Chaves Dos; Lima, Glauber Cruz; Aragão, Kalynca Kayla Viana; Vasconcelos, Renata Prado; Araújo, Pâmella Cristina da Costa; Vasconcelos, Yuri de Abreu Gomes; Oliveira, Ariclécio Cunha de; Oliveira, Hermógenes David de; Portella, Viviane Gomes; Coelho-de-Souza, Andrelina Noronha

    2013-10-07

    Hyptis crenata Pohl ex Benth (Lamiaceae), popularly known as "hortelã-brava" or "hortelã do campo", is widely distributed in the northeast of Brazil. In Brazil, the leaves of this plant have been used in traditional medicine for the treatment of gastrointestinal disturbances, including gastric ulcers. In an attempt to experimentally validate this claimed antiulcerogenic activity, the gastroprotective effects of the essential oil extracted from the leaves of the Hyptis crenata Pohl ex Benth (EOHc) were evaluated in recognized gastric ulcer models in mice. EOHc was obtained by hydrodistillation and analyzed using gas chromatography-mass spectrometry (GC-MS). Swiss male mice (25-30g) were used for the studies. The gastric ulcers were induced by oral administration of absolute ethanol or indomethacin 45min after oral pretreatment with EOHc, vehicle and positive control drugs. One hour after the ulcerative challenges, the stomachs were removed and the area of the lesions was measured. The volume, pH and total acidity of the gastric secretions were determined using the pylorus ligature model. The gastrointestinal motility was measured using gastric emptying and intestinal transit. The ethanol-induced gastric mucus depletion and lipid peroxidation were also analyzed. Our findings are as follows: A significant inhibition of gastric lesions induced by absolute ethanol was observed in the mice pre-treated with EOHc, at a dose of 30 and 100 and 300mg/kg (5.56±1.51, 2.88±0.82 and 1.71±0.54mm(2), respectively) compared to control group (118.03±35.4mm(2)). Also, EOHc (300mg/kg) produced a gastroprotective effect against the gastric lesions induced by indomethacin (16.07±4.68mm(2)) compared to control group (38.64±6.1mm(2)). EOHc pretreatment produced a reduction in the ethanol-induced lipid peroxidation from 3.9±0.22 to 2.4±0.1μmol/mg tissue (EOHc-300mg/kg and control group, respectively). We also observed that EOHc pretreatment decreased the gastric emptying, but did

  2. Laxative activities of Mareya micrantha (Benth. Müll. Arg. (Euphorbiaceae leaf aqueous extract in rats

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    Djaman Joseph A

    2010-02-01

    Full Text Available Abstract Background Mareya micrantha (Benth. Müll. Arg. (Euphorbiaceae is a shrub that is commonly used in Côte d'Ivoire (West Africa for the treatment of constipation and as an ocytocic drug. The present study was carried out to investigate the laxative activity of Mareya micrantha in albino's Wistar rats. Methods Rats were divided in 5 groups of 5 animals each, first group as control, second group served as standard (sodium picosulfate while group 3, 4 and 5 were treated with leaf aqueous extract of Mareya micrantha at doses of 100, 200 and 400 mg/kg body weight (b.w., per os respectively. The laxative activity was determined based on the weight of the faeces matter. The effects of the leaves aqueous extract of Mareya micrantha and castor oil were also evaluated on intestinal transit, intestinal fluid accumulation and ions secretion. Results Phytochemicals screening of the extract revealed the presence of flavonoids, alkaloids, tannins, polyphenols, sterols and polyterpenes. The aqueous extract of Mareya micrantha applied orally (100, 200 and 400 mg/kg; p.o., produced significant laxative activity and reduced loperamide induced constipation in dose dependant manner. The effect of the extract at 200 and 400 mg/kg (p.o. was similar to that of reference drug sodium picosulfate (5 mg/kg, p.o. The same doses of the extract (200 and 400 mg/kg, p.o. produced a significant increase (p -, Na+, K+ and Ca2+ in the intestinal fluid (p Conclusions The results showed that the aqueous extract of Mareya micrantha has a significant laxative activity and supports its traditional use in herbal medicine.

  3. Adaptations to soil drying in woody seedlings of African locust bean, (Parkia biglobosa (Jacq.) Benth.).

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    Osonubi, O; Fasehun, F E

    1987-12-01

    Stomatal conductance, transpiration and xylem pressure potential of African locust bean (Parkia biglobosa (Jacq.) Benth.) seedlings subjected from the sixth week after emergence to four weeks of continuous soil drought did not differ from those of well-watered, control plants until two-thirds of the available soil water had been used. In both well-watered and drought-treated plants, stomatal conductance was highest early in the day when vapor pressure deficits were low, but decreased sharply by midday when evaporative demand reached its highest value. There was no increase in stomatal conductance later in the day as vapor pressure deficit declined. The relationship between transpiration rate and xylem pressure potential showed non-linearity and hysteresis in both control and drought-treated plants, which seems to indicate that the plants had a substantial capacity to store water. The rate of leaf extension in African locust bean seedlings subjected to six consecutive 2-week cycles of soil drought declined relative to that of well-watered, control plants, whereas relative root extension increased. It appears that African locust bean seedlings minimized the impact of drought by: (1) restricting transpiration to the early part of the day when a high ratio of carbon gain to water loss can be achieved; (2) utilizing internally stored water during periods of rapid transpiration; (3) reducing the rate of leaf expansion and final leaf size in response to soil drought without reducing the rate of root extension, thereby reducing the ratio of transpiring leaf surface area to absorbing root surface area.

  4. Antifungal Screening of Bridelia ferruginea Benth (Euphorbiaceae Stem Bark Extract in Mouthwash Formulations

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    Aremu Olusola Isaac

    2017-06-01

    Full Text Available The plant Bridelia ferruginea Benth (Euphorbiaceae has been known for its use in the management of oral thrush ethnomedicinally in various parts of Africa, a practice which has been justified by results of certain scientific studies. The aim of this study was to develop an appropriate dosage formulation, a mouthwash and evaluate the antifungal potential of this dosage formulation against a major causative organism of oral thrush, Candida albicans. Extraction of the stem bark was carried out with boiled distilled water, the extract was formulated into mouthwashes at concentrations of 0.5, 1.0, 1.5, 2.0 and 2.5%w/v. All formulations contained viscosity imparting agent, a sweetener and a preservative. Physical characterisation, viscosity, pH and palatability of the mouthwash formulations were determined. Agar-well diffusion method was used to assess antifungal activity of the formulations against Candida albicans and Nystatin oral suspension was used as reference compound. The results showed that Bridelia ferruginea stem bark extract mouthwash solutions were brown in colour, had agreeable odour and sweet astringent taste. The pH for all concentrations was in the range 5.41-5.63. The viscosity at spindle no 2, 60rpm range between 0.226-0.238 Pa.S for all concentrations studied. The formulations had antifungal activity against Candida albicans. The highest concentration (2.5%w/v gave mean zone of inhibition of 25.50±0.71mm that was comparable with Nystatin oral suspension 28.00±1.41mm, a reference compound. The foregoing suggests that with little modification in the formulation especially the adjustment of the pH, Bridellia ferruginea mouthwash solutions may be developed into commercially useful preparations.

  5. Salvia leriifolia Benth (Lamiaceae) extract demonstrates in vitro antioxidant properties and cholinesterase inhibitory activity.

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    Loizzo, Monica R; Tundis, Rosa; Conforti, Filomena; Menichini, Federica; Bonesi, Marco; Nadjafi, Farsad; Frega, Natale Giuseppe; Menichini, Francesco

    2010-12-01

    The object of the present study was to investigate the in vitro antioxidant properties and cholinesterase inhibitory activity of Salvia leriifolia Benth extracts and fractions. The functional role of herbs and spices and their constituents is a hot topic in food-related plant research. Salvia species have been used since ancient times in folk medicine for cognitive brain function and have been subjected to extensive research. Thus, we hypothesize that S leriifolia, because of its functional properties, would be a good candidate to use as a nutraceutical product for improving memory in the elderly or patients affected by Alzheimer disease (ad). To test this hypothesis, we examined the cholinesterase inhibitory activity using the modified colorimetric Ellman's method against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). The n-hexane exhibited the highest activity, with inhibitory concentration 50% (IC(50)) values of 0.59 and 0.21 mg/mL, for AChE and BChE, respectively. This extract was fractionated, and 9 of these fractions (A-I) were obtained and tested. Fraction G, characterized by the presence of sesquiterpenes as major components, was the most active against AChE (IC(50) = 0.05 mg/mL). Because oxidative stress is a critical event in the pathogenesis of AD, we decided to screen the antioxidant activity (AA) using 2,2-diphenyl-1-picrylhydrazyl test, β-carotene bleaching test, and bovine brain peroxidation (thiobarbituric acid) assay. The ethyl acetate extract showed the highest activity, with IC(50) values of 2 and 33 μg/mL on β-carotene bleaching test and thiobarbituric acid test, respectively. These results suggest potential health benefits of S leriifolia extracts. However, this finding requires additional investigation in vivo.

  6. Chemical Composition and Allelopathic Potential of Essential Oils from Tipuana tipu (Benth.) Kuntze Cultivated in Tunisia.

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    El Ayeb-Zakhama, Asma; Sakka-Rouis, Lamia; Bergaoui, Afifa; Flamini, Guido; Jannet, Hichem Ben; Harzallah-Skhiri, Fethia

    2016-03-01

    In Tunisia, Tipuana tipu (Benth.) Kuntze is an exotic tree, which was introduced many years ago and planted as ornamental street, garden, and park tree. The present work reported, for the first time, the chemical composition and evaluates the allelopathic effect of the hydrodistilled essential oils of the different parts of this tree, viz., roots, stems, leaves, flowers, and pods gathered in the area of Sousse, a coastal region, in the East of Tunisia. In total, 86 compounds representing 89.9 - 94.9% of the whole oil composition, were identified in these oils by GC-FID and GC/MS analyses. The root essential oil was clearly distinguished for its high content in sesquiterpene hydrocarbons (β-caryophyllene, 1 (44); 24.1% and germacrene D, 2 (53); 20.0%), while those obtained from pods, leaves, stems, and flowers were dominated by non-terpene hydrocarbons. The most important ones were n-tetradecane (41, 16.3%, pod oil), 1,7-dimethylnaphthalene (43, 15.6%, leaf oil), and n-octadecane (77, 13.1%, stem oil). The leaf oil was rich in the apocarotene (E)-β-ionone (4 (54); 33.8%), and the oil obtained from flowers was characterized by hexahydrofarnesylacetone (5 (81); 19.9%) and methyl hexadecanoate (83, 10.2%). Principal component and hierarchical cluster analyses separated the five essential oils into three groups and two subgroups, each characterized by the major oil constituents. Contact tests showed that the germination of lettuce seeds was totally inhibited by the root essential oil tested at 1 mg/ml. The inhibitory effect on the shoot and root elongation varied from -1.6% to -32.4%, and from -2.5% to -64.4%, respectively.

  7. Gastroprotective and ulcer healing effects of essential oil of Hyptis martiusii Benth. (Lamiaceae.

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    Germana Freire Rocha Caldas

    Full Text Available Hyptis martiusii Benth. is an aromatic plant found in abundance in northeastern Brazil that is used in ethnomedicine to treat gastric disorders. The aim of this study was to elucidate the mechanisms of action involved in the gastroprotection of the essential oil of Hyptis martiusii (EOHM and to evaluate its healing capacity. Wistar rats were exposed to different protocols and subsequently were treated with 1% Tween-80 aqueous solution (negative control, pantoprazole, carbenoxolone, N-acetylcysteine (depending on the specificity of each model or EOHM. The antisecretory activity (basal or stimulated was determined using the pyloric ligature method. The gastroprotective action of nitric oxide and sulphydryl groups (-SH groups, as well as the quantification of adherent mucus and the levels of malondialdehyde and -SH groups in gastric mucosa, were evaluated using ethanol-induced gastric ulcer model. The healing ability was evaluated using the acetic acid-induced gastric ulcer model and histological and immunohistochemical analysis (HE, PAS and PCNA. EOHM (400 mg/kg reduced the volume and acidity of gastric secretion stimulated by histamine and pentagastrin. The gastroprotective effect of EOHM involves the participation of endogenous sulfhydryl groups. EOHM increased mucus production (54.8%, reduced levels of MDA (72.5% and prevented the depletion of -SH groups (73.8% in the gastric mucosa. The treatment with EOHM reduced in 70.3% the gastric lesion area, promoting significant regeneration of the gastric mucosa, as confirmed by histological analysis and analysis of proliferating cell nuclear antigen. The results show that gastroprotective effect of EOHM is mediated by cytoprotective and antioxidant mechanisms and by their antisecretory activity, and suggest that the essential oil of Hyptis martiusii is a promising candidate for the treatment of gastric ulcers.

  8. Mechanism of antiulcerogenic activity of semi-synthetic crotonin obtained from Croton cajucara Benth.

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    Ana Beatriz A. Almeida

    2002-01-01

    Full Text Available The bark of Croton cajucara Benth. is used in Brazilian folk medicine to treat gastrointestinal disorders. Transdehydrocrotonin (DHC isolated from the bark of Croton cajucara has antiulcerogenic activity25. The presence of similar activity in semi-synthetic crotonin obtained from dehydrocrotonin from Croton cajucara was observed in gastric ulcer-induced models (HCl/ethanol, ethanol, indomethacin, stress and pylorus ligature. The aim of the present study was to assess the mechanisms involved in the antiulcerogenic activity of semi-synthetic crotonin. We investigated the effects of semi-synthetic crotonin on the response to histamine of right atria isolated from guinea pigs and on the response to carbachol of stomach fundus strips from rats. Semi-synthetic crotonin (3, 10 or 30 mM induced a shift to the right in the concentrationresponse curves to carbachol in the isolated rat stomach at the pD2 level (pD2: 5.42±0.05, 5.76±0.061, 5.77±0.076, 6.48±0.012, respectively, without any alteration in the maximum response. Semi-synthetic crotonin also induced a shift to the right in the concentration-response curves to histamine in guinea pig right atria, pD2 (5.54±0.06, 6.01±0.06, 5.89±0.06, 5.92±0.03 and (% maximum response (80±6.18, 118±6.18, 114±6.18, 122±1.4, respectively. Thus, the protective effect of semi-synthetic crotonin on induced gastric lesions could be due to antagonism of histaminergic and cholinergic effects on gastric secretion.

  9. Antiplasmodial activity of sesquiterpene lactones and a sucrose ester from Vernonia guineensis Benth. (Asteraceae)

    Science.gov (United States)

    Toyang, Ngeh J.; Krause, Michael A.; Fairhurst, Rick M.; Tane, Pierre; Bryant, Joseph; Verpoorte, Rob

    2013-01-01

    Ethnopharmacological relevance Aqueous preparations of Vernonia guineensis Benth. (Asteraceae) are used in Cameroonian folk medicine as a general stimulant and to treat various illnesses and conditions including malaria, bacterial infections and helminthic infestations. Materials and methods 10-g samples of the leaf and tuber powders of V. guineensis were extracted separately using dichloromethane, methanol and distilled water. The extracts were dried in vacuo and used in bioassays. These extracts and three compounds previously isolated from V. guineensis [vernopicrin (1), vernomelitensin (2) and pentaisovalerylsucrose (3)] were screened for antiplasmodial activity against chloroquine (CQ)-sensitive (Hb3) and CQ-resistant (Dd2) Plasmodium falciparum lines. Results Crude extracts and pure compounds from V. guineensis showed antiplasmodial activity against both Hb3 and Dd2. The IC50 values of extracts ranged from 1.64 – 27.2 μg/ml for Hb3 and 1.82 – 30.0 μg/ml for Dd2; those for compounds 1, 2 and 3 ranged from 0.47 – 1.62 μg/ml (1364 – 1774 nM) for Hb3 and 0.57 – 1.50 μg/ml (1644 – 2332 nM) for Dd2. None of the crude extracts or pure compounds was observed to exert toxic effects on the erythrocytes used to cultivate the P. falciparum lines. Conclusion In Cameroonian folk medicine, V. guineensis may be used to treat malaria in part due to the antiplasmodial activity of sesquiterpene lactones (1, 2), a sucrose ester (3) and perhaps other compounds present in crude plant extracts. Exploring the safety and antiplasmodial efficacy of these compounds in vivo requires further study. PMID:23542146

  10. Echophytochemical, Antioxidant and Ethnopharmacological Properties of Stachys inflata Benth.Extract from Chahar Bagh Mountain

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    Amir Mohammadi (MSc

    2016-06-01

    Full Text Available BACKGROUND AND OBJECTIVE: Stachys inflata Benth. is used as an anti-inflammatory and antiseptic agent in traditional medicine in most mountain villages of Golestan province. Therefore, this study aimed to investigate the antioxidant, ethnopharmacological and phytochemical properties of extract from different parts of S. inflata, collected from Chahar Bagh Mountain. METHODS: Flowering branches and root of the plant were collected from Chahar Bagh Mountain (2100 m in July 2013. At the same time, the most important information about traditional uses of the plant (ethnopharmacology was recorded by questioning local people. Phytochemical evaluation (total phenolic, flavonoid and anthocyanins content of ethanolic extract of plant organs was done using spectrophotometry and folin-ciocalteu. The antioxidant activity of the extract was evaluated by DPPH test. P ≤0.05 was considered as statistically significant. RESULTS: The amount of chemical compounds in the extract of flowering branches and root extract was significantly different. The total phenolic (129.96 ± 5.6 mgGAE/g, flavonoid (29.62 ± 1.4 mgQUE/g and anthocyanin (0.021 ± 0.001 µg/g content in the extract of aerial parts of the plant was approximately 1.5 to 3 times higher than those in the root. Due to higher production of active compounds, the antioxidant activity of the aerial parts’ extract showed a greater potential in free radical scavenging (IC50= 76.33 ± 4.2 µg/ml compared to the root extract. CONCLUSION: Phytochemical findings and antioxidant activity of the extract of aerial parts of the plant in free radical scavenging, confirm the traditional applications of this plant as analgesic, anti-inflammatory and antiseptic agent in treatment of rheumatism, wounds, burns and diarrhea. It is recommended that further evaluation of the plant’s traditional applications be conducted in vivo and in vitro.

  11. [Investigation of determining strontium in M. nitida Benth. var. hirsutissima. Z. Wei. by flame atomic absorption spectra].

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    Zhang, Ling; Rao, Zhi-Jun; Guan, Yuan-Yuan; Zhang, Hai-Ming

    2010-12-01

    The present paper is aimed to establish the method of determining the strontium in M. nitida Benth. var. hirsutissima. Z. Wei. by means of air-acetylene flame atomic absorption spectra, and also provide reference for the determination of the strontium in other traditional Chinese medicine. M. Nitida Benthvarhirsutissima Z. Wei. was taken as the object. The authors used nitric-perchloric acid as digestion solution to digest samples by microwave which was controlled by pressure, and used EDTA-2Na as the releasing agent to add in the samples for determining the strontium in M. nitida Benth. var. hirsutissima. Z. Wei. by FAAS. The results showed that the samples were entirely digested by microwave. The working curve was Y = 0.036 5x -0.001 1, r = 0.999 4, the range was 0-1.6 microg x mL(-1), the average recovery rate was 101.5% with RSD 2.04%, and the method detection limit was 0.008 2 microg x mL(-1) (n = 21). It is concluded that this method is simple and accurate. It has high sensitivity and can be effectively used for determining the strontium in this traditional Chinese medicine.

  12. MIMO/SA Multi-antenna Space-time Channel Modeling%MIMO/SA多天线空时信道建模

    Institute of Scientific and Technical Information of China (English)

    吕郡陵; 郭爱煌

    2012-01-01

    Based on correlation matrix method. Multiple-input and Multiple-output(MIMO) channel coefficient matrix can be obtained by calculating the correlation coefficient between two antennas and MIMO correlation matrix. The steering vector and beam-forming vector of Smart Antenna(SA) are added to the MIMO channel coefficient matrix to form MIMO/SA channel matrix, so that MIMO/SA multi-antenna space-time channel is modeled. A channel simulation platform is established to simulate and analyze MIMO/SA channel in time domain, frequency domain and space domain. Simulation results show that the model has a good channel directivity gain and the channel properties in space, time and frequency meet the demands of 3GPP TR 25.996, and it is useful for designing and optimizing MIMO/SA multi-antenna system and the analysis of channel capacity.%运用相关矩阵法,通过计算2根天线之间的相关系数、多输入多输出(MIMO)的相关矩阵,得到MIMO信道系数矩阵,在其中加入智能天线(SA)的导向向量和赋形向量,得到MIMO/SA矩阵,由此实现对MIMO/SA多天线空时信道的建模.利用Matlab搭建一个仿真平台,从空间、时间、频率3个方面分析MIMO/SA多天线信道模型,结果表明,该模型具有较好的信道方向性增益,信道的空时频特性符合3GPP 25.996的要求,可用于MIMO/SA多天线的设计、优化和信道容量分析.

  13. Genetic diversity of Mimosa pudica rhizobial symbionts in soils of French Guiana: investigating the origin and diversity of Burkholderia phymatum and other beta-rhizobia.

    Science.gov (United States)

    Mishra, Ravi P N; Tisseyre, Pierre; Melkonian, Rémy; Chaintreuil, Clémence; Miché, Lucie; Klonowska, Agnieszka; Gonzalez, Sophie; Bena, Gilles; Laguerre, Gisèle; Moulin, Lionel

    2012-02-01

    The genetic diversity of 221 Mimosa pudica bacterial symbionts trapped from eight soils from diverse environments in French Guiana was assessed by 16S rRNA PCR-RFLP, REP-PCR fingerprints, as well as by phylogenies of their 16S rRNA and recA housekeeping genes, and by their nifH, nodA and nodC symbiotic genes. Interestingly, we found a large diversity of beta-rhizobia, with Burkholderia phymatum and Burkholderia tuberum being the most frequent and diverse symbiotic species. Other species were also found, such as Burkholderia mimosarum, an unnamed Burkholderia species and, for the first time in South America, Cupriavidus taiwanensis. The sampling site had a strong influence on the diversity of the symbionts sampled, and the specific distributions of symbiotic populations between the soils were related to soil composition in some cases. Some alpha-rhizobial strains taxonomically close to Rhizobium endophyticum were also trapped in one soil, and these carried two copies of the nodA gene, a feature not previously reported. Phylogenies of nodA, nodC and nifH genes showed a monophyly of symbiotic genes for beta-rhizobia isolated from Mimosa spp., indicative of a long history of interaction between beta-rhizobia and Mimosa species. Based on their symbiotic gene phylogenies and legume hosts, B. tuberum was shown to contain two large biovars: one specific to the mimosoid genus Mimosa and one to South African papilionoid legumes. © 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

  14. Optimization of Extracting Technology for Essential Oil from Elsholizia densa Benth%超声辅助提取密花香薷精油工艺优化

    Institute of Scientific and Technical Information of China (English)

    吕蒙蒙; 吴夏; 谢云芳; 穆小珍; 康淑荷

    2013-01-01

    [目的]优选密花香薷精油最佳提取工艺条件.[方法]采用超声辅助水蒸气蒸馏,通过正交试验考察了药材粒度、超声时间、料液比、浸泡时间4因素3水平对密花香薷精油得率的影响.[结果]密花香薷精油提取的最佳工艺条件为药材粒度50目,超声时间12min,浸泡时间6h,料液比1∶16 g/ml.在该工艺条件下,精油的平均得率为0.407%.[结论]优选的密花香薷精油提取工艺得率较高,条件合理,简单可行.%[ Objective] The study aims to optimize the extracting technology conditions of essential oil from Elsholtzia densa Benth. [ Method] Volatile oil was extracted from Elsholtzia densa Benth using steam distillation , and the effects of four extracting factors (the size of the medicinal herb, ultrasonic time, soaking time and the ratio of the material to liquid) on the yield of essential oil extracted from Elsholtzia densa Benth was analyzed through the orthogonal test method. [ Result] The optimized extraction process of essential oil from Elsholtzia densa Benth was as follows: the size of the medicinal herb 50 mesh, ultrasonic time 12 min, being soaked in the water for 6 h , and the ratio of the material to liquid 1 : 16 g/ml. Under this conditions, the average extracting rate of the essential oil was 0. 407%. [ Conclusion ] The optimized extraction process of essential oil from Elsholtzia densa Benth was simple and practicable, and its extracting rate was higher.

  15. Constituintes químicos, avaliação das atividades citotóxica e antioxidante de Mimosa paraibana Barneby (Mimosaceae Chemical constituents, evaluation of the cytotoxic and antioxidant activities of Mimosa paraibana Barneby (Mimosaceae

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    Xirley P. Nunes

    2008-12-01

    Full Text Available Mimosa paraibana Barneby foi submetida a um estudo fitoquímico para o isolamento de seus constituintes químicos, através de métodos cromatográficos usuais, e posterior identificação estrutural, utilizando-se métodos espectroscópicos de RMN ¹H e 13C uni e bidimensionais, além de comparações com modelos da literatura. Deste estudo pioneiro, foram isolados e identificados cinco constituintes da fase clorofórmica: uma mistura dos esteróides, β-sitosterol e estigmasterol, a 15¹-hidroxi-feofitina A, a 5,7-dihidroxiflavanona, o 3,4,5-trihidroxibenzoato de etila e o ácido p-cumárico. A atividade antioxidante das fases hexânica, clorofórmica e acetato de etila foi avaliada utilizando o radical estável DPPH (1,1-difenil-2-picril-hidrazil e os resultados comparados com o padrão ácido ascórbico. A avaliação da citotoxicidade das fases foi realizada empregando-se o ensaio de letalidade contra Artemia salina. Dos extratos avaliados, somente o hexânico mostrou baixa toxicidade.The phytochemical study of Mimosa paraibana Barneby led to the isolation of its chemical constituents, through the usual chromatographic methods, and further structural identification, using ¹H and 13C NMR spectroscopic methods based on one and two-dimensional techniques, in addition to comparison with literature data. From this pioneering investigation with M. paraibana, five constituents were isolated and identified from the chloroform extract: a mixture of β-sitosterol and stigmasterol, 15¹-hydroxy-phaeophytin A, 5,7-dihydroxyflavanone, ethyl 3,4,5-trihydroxybenzoate and p-coumaric acid. The antioxidant activity of the hexane, chloroform and ethyl acetate extracts of M. paraibana were measured using the 1,1-diphenyl-2-picryl-hydrazyl (DPPH free radical scavenging assay and the results compared with standard ascorbic acid. The toxicity activity of the extracts were performed using the bioassay of Artemia salina.

  16. Atividade biológica da jurema-preta (Mimosa tenuiflora (Wild Poir. sobre Staphylococcus aureus isolado de casos de mastite bovina Biological Activity of jurema preta (Mimosa tenuiflora (Wild Poiret on Staphylococcus aureus isolated of mastitis cattle

    Directory of Open Access Journals (Sweden)

    Denise A. C. Bezerra

    2009-12-01

    Full Text Available O objetivo deste estudo foi avaliar a sensibilidade do Staphylococcus aureus ao extrato da jurema-preta (Mimosa tenuiflora (Wild Poir.. Foram isoladas 25 cepas de S. aureus de amostras de leite de vacas com histórico de mastite clínica e subclínica, submetidas ao teste de atividade antimicrobiana do extrato de M. tenuiflora. Os testes de sensibilidade in vitro foram realizados utilizando o método de difusão em meio sólido. Em seguida foram inoculados 50 μL do extrato nas seguintes diluições 1:1; 1:2; 1:4; 1:8; 1:16; 1:32; 1:64; 1:128; 1:256; 1:512. As placas foram incubadas a 37 °C, por um período de 24 a 48 h. Os ensaios foram realizados em triplicata. Observou-se halos de inibição entre 6 e 25 mm de diâmetro. O percentual de sensibilidade das amostras testadas foi distribuido em: 1:1 a 1:32 (100%; 1:64 (92%; 1: 128 (72%; 1:256 (28%; 1:512 (0%. O estudo da M. tenuiflora sobre as amostras de S. aureus testadas demonstrou que a planta tem ação antimicrobiana.The aim of this study was to evaluate the sensitivity of the S.aureus to the extract of Mimosa tenuiflora (Wild Poir. (jurema-preta. Twenty five strains of S. aureus were isolated from milk samples with description of clinical and subclinical mastitis and submitted to antimicrobial activity test of the M. tenuiflora extract. The sensitivity in vitro tests were carried out using the agar difusion method (well diffusion method. After that, 50μL of the extract were inoculated in the following dilutions: 1:1; 1:2; 1:4; 1:8; 1:16; 1:32; 1:64; 1:128; 1:256; 1:512. The plates were incubated at 37 ºC during 24 and 48 h. The tests were made in triplicate. Halos of inhibition were observed between 6 and 25 mm of diameter. The percentage of sensitivity of the tested samples was distributed in: 1:1 to 1:32 (100%, 1:64 (92%; 1: 128 (72%; 1:256 (28%; 1:512 (0%. The study of M. tenuiflora on the tested S. aureus samples it demonstrated that the plant has antimicrobial action.

  17. Superação da dormência em sementes de faveira (Parkia platycephala Benth Dormancy breaking of faveira (Parkia platycephala Benth. seeds

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    Irinaldo Lima do Nascimento

    2009-02-01

    Full Text Available A faveira é planta semidecídua, heliófita, que ocorre em formações secundárias e áreas abertas de terreno elevado do agreste nordestino e campinas amazônicas. A madeira dessa espécie é empregada para caixotaria, compensados, brinquedos, lenha e carvão, as vagens maduras constituem-se em excelente forragem para todos os ruminantes e a árvore é recomendada para arborização paisagística. O primeiro problema encontrado foi a baixa germinação das sementes devido à impermeabilidade do tegumento à água. Dessa forma, este trabalho teve o objetivo de determinar a metodologia mais eficiente para superação da dormência de sementes de Parkia platycephala, as quais foram submetidas a 12 tratamentos: testemunha - sementes intactas (T1, escarificação mecânica com lixa d'água n. 80 (T2, imersão em ácido muriático concentrado (98% por 30 min e 1 h (T3 e T4, respectivamente, escarificação mecânica com brita por 5, 10 e 15 min (T5, T6 e T7, respectivamente e imersão em ácido sulfúrico concentrado (98% por 5, 15, 30, 45 e 60 min (T8, T9, T10, T11 e T12, respectivamente. Os efeitos dos tratamentos foram avaliados através da porcentagem, primeira contagem e índice de velocidade de emergência de plântulas, além de comprimento e massa seca da raiz e parte aérea. Utilizou-se o delineamento inteiramente casualizado com quatro repetições, e as médias foram comparadas pelo teste de Tukey a 5% de probabilidade. Houve diferença significativa entre os tratamentos em todas as variáveis. A causa mais evidente da dormência foi a impermeabilidade do tegumento, cujos tratamentos mais eficientes para superar a dormência das sementes foram a escarificação mecânica do tegumento com lixa e a imersão em ácido sulfúrico (15 a 45 min.The faveira (Parkia platycephala Benth. is a perennial, heliophyte, secondary plant that occurs in (non-flooded areas of the Amazon area. The wood of this species is used to make boxes, supports and

  18. Antitrypanosomal activity of Verbascum sinaiticum Benth. (Scrophulariaceae) against Trypanosoma congolense isolates.

    Science.gov (United States)

    Mergia, Ermias; Shibeshi, Workineh; Terefe, Getachew; Teklehaymanot, Tilahun

    2016-09-15

    African Trypanosomiasis is a neglected tropical disease with a large impact on the livelihood of the rural poor in Sub-Saharan Africa. The available drugs for managing this disease are old, expensive and are facing the problem of drug resistance. Thus, the aim of this study was to evaluate in vivo antitrypanosomal efficacy of aqueous and absolute methanol leaf extracts of Verbascum sinaiticum Benth. against Trypanosoma congolense field isolate. Verbascum sinaiticum (Local name 'qetetina') is a biennial plant, and 60-150 cm tall. It is traditionally used to treat wound, stomachache, viral infection, cancer, sunstroke, fever, abdominal colic, diarrhea, hemorrhage, anthrax, and hepatitis. The efficacy of aqueous and absolute methanol leaf extracts of V. sinaiticum was evaluated in a randomized experiment with Swiss albino mice infected with T. congolense field isolate. The extracts were administered at doses of 100, 200 and 400 mg/kg by intraperitoneal injection for seven days at 12 Days Post-Infection (DPI) when the peak parasitaemia level was approximately 10(8) trypanosomes/ml. Parasitaemia, Packed Cell Volume (PCV), mean survival time and change in body weight were used as indices for monitoring the efficacy of the extracts. Diminazene (28 mg/kg) was used as a positive control while 2 % Tween was used as the negative control. Phytochemicals screening were conducted following standard methods. The extracts showed no toxicity effect in Swiss albino mice and had LD50 above 2000 mg/kg. The phytochemicals screened in V. sinaiticum were alkaloids, flavonoids, glycoside, saponins, steroids, phenolic compounds, and tannins. The mice treated with absolute methanol leaf extract of V. sinaiticum at 400 mg/kg dose had significantly lower mean parasitaemia (7.20 ± 0.16) (p < 0.001) as compared to the negative control group (8.82 ± 0.12) on day 14 of treatment. Animals treated with the same dose had significant (p < 0.001) higher PCV value and body

  19. Escarificación artificial y natural de la semilla de Lupinus bilineatus Benth

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    J. M. Martínez

    2008-01-01

    Full Text Available Las especies de Lupinus colonizan áreas incendiadas y fijan N. En otros países las especies de este género se emplean en plantaciones forestales y sistemas agroforestales. Sus semillas presentan latencia física. En el presente estudio se realizó el análisis de semillas y se evaluó la germinación de Lupinus bilineatus Benth. considerando los siguientes factores y niveles: escarificación (natural: fuego, humo; artificial: inmersión en ácido sulfúrico durante 10, 20, 30 y 40 min y control, régimen de temperatura día/noche en cámaras de ambiente controlado (15/10, 20/15, 25/20, 30/25 °C y luz (con y sin. El diseño experimental fue en bloques al azar, con los bloques anidados dentro del factor temperatura. Se realizó un análisis de varianza mixto (con el procedimiento mixto de SAS y la prueba de Tukey. La pureza fue 97.1 %, el peso 66,666.7 semillas·kg-1, el contenido de humedad (base anhidra 7.1 % y la viabilidad 98 % (prueba de tetrazolio. Para la germinación, resultó significativa (P=0.0101 la interacción entre tratamiento escarificatorio, temperatura y luz. La máxima germinación (82.5 % se registró para semilla escarificada químicamente durante 30 o 40 min, a 25/20 °C y con luz. A mayor tiempo de escarificación química, mayor porcentaje de germinación. A 20/15 °C y con luz, la germinación posterior a la escarificación con fuego (38.5 % superó al control (18.5 %. Generalmente el control mostró valores de germinación menores a 30 % en los diferentes tratamientos, denotando latencia física con diferentes intensidades en el lote. Destaca la eficiencia de la escarificación química para cesar la latencia.

  20. Surtos de dermatite ulcerativa causados por espinhos de Mimosa setosa, M. debilis e M. pudica (Fabaceae em equinos

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    Saulo A. Caldas

    Full Text Available RESUMO: Descrevem-se, na pele de equídeos, lesões de natureza mecânico-traumática causadas por espinhos de Mimosa spp. Dentre as três espécies da planta identificadas como responsáveis pelas lesões, M. setosa estava presente em maior quantidade (80% e M. debilis e M. pudica encontravam-se em menor proporção na pastagem. Ocorreram três surtos de dermatite ulcerativa em períodos chuvosos de abril a maio de 2013, dezembro de 2013 a fevereiro de 2014 e abril a maio deste mesmo ano. Vinte e cinco equinos do Setor de Reprodução Animal da Universidade Federal Rural do Rio de Janeiro apresentaram, na pele, lesões ulcerativas com contornos irregulares, exsudato hemorrágico, recobertas por crostas. As lesões localizavam-se sobretudo nas regiões de quartela, boleto, articulação escapulo-umeral, lábios superior e inferior, focinho, narinas, bochechas e chanfro. Sete animais foram biopsiados e o exame histopatológico revelou ulceração da epiderme e infiltrado inflamatório constituído por macrófagos e neutrófilos, delimitado por tecido de granulação subjacente. Em alguns casos, foram observados microespículos das referidas plantas (tricomas hirsutos em meio à reação inflamatória. O diagnóstico de dermatite cutânea causada pela ação traumática da planta baseou-se na presença de Mimosa spp. na pastagem, nas características e localização das lesões na pele dos equinos, nos achados histopatológicos e na recuperação após a retirada dos animais do pasto.

  1. Effect of Four Kinds of Environment Factors on Seed Germination of Cenchrus pauciflorus Benth%4种环境因子对少花蒺藜草(Cenchrus pauciflorus Benth.)种子萌发的影响

    Institute of Scientific and Technical Information of China (English)

    曲波; 朱明星; 王巍; 韩志松; 吕林有; 李天来

    2011-01-01

    本试验以少花蒺藜草种子为研究材料,通过设定不同的温度、土壤湿度、土壤基质和光照等条件研究环境因子对少花蒺藜草种子萌发的影响.结果表明:少花蒺藜草种子萌发最适温度为25℃,萌发最低温度为20℃,萌发最高温度为30℃;光照对少花蒺藜草种子萌发有一定影响,光照促进种子萌发;土壤湿度对其萌发有影响,最适萌发湿度为20%,最低萌发湿度为6%;少花蒺藜草种子萌发对土壤基质要求不严格,于沙土及草炭土中均可萌发.%With Cenchrus pauciflorus Benth. seed as material to study the effect of different emperature, soil moisture, soil substrate and illumination on seed germination. The result showed that temperature had great influence on seed germination of Cenchrus pauciflorus Benth, and the lowest germination at 20 ℃, and the highest germination rate at 30 ℃ ,and the best suitable germination temperature was 25 ℃. Light could improve seed germination. Soil moisture had great influence on seed germination, the best humidity was 20%, the minimum humidity was 6%. Soil substrate had little effect on seed germination, and seed could germination in sand and turfy soil.

  2. In vitro anti-staphylococcal activity of Hyptis martiusii Benth against methicillin-resistant Staphylococcus aureus: MRSA strains Atividade anti-estafilocócica in vitro de Hyptis martiusii Benth contra linhagens de Staphylococcus aureus resistentes à meticilina: MRSA

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    Henrique D. M. Coutinho

    2008-12-01

    Full Text Available This is the first report about the antibacterial activity of Hyptis martiusii Benth. In this study the ethanol extract of H. martiusii was tested for its antimicrobial activity against strains of Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. The growth of all bacterial strains tested was inhibited by the extract. The diameter of inhibition zones varied from 13 to 20 mm for the extract. The MIC and MBC values ranged from 128 to > 1024mg/mL and 256 to > 1024 mg/mL, respectively. It is therefore suggested that extracts from H. martiusii could be used as an anti-Staphylococcus agent. Compared with methicillin and gentamicin, the extract was more effective, being a promising antibacterial agent.Este é o primeiro relato de atividade antibacteriana de Hyptis martiusii Benth. Neste estudo, o extrato etanólico de H. martiusii foi avaliado para atividade antimicrobiana contra linhagens de Escherichia coli, Pseudomonas aeruginosa e Staphylococcus aureus. O crescimento de todas as bactérias testadas foi inibido pelo extrato. O diâmetro das zonas de inibição variaram de 13 - 20 mm. Os valores da CIM e CBM variaram de 128 a > 1024 mg/mL e 256 a > 1024 mg/mL, respectivamente. Devido a isso, podemos indicar que o extrato etanólico de H. martiusii pode ser usado como um agente anti-Staphylococcus. Quando comparado com outros antibióticos como meticilina e gentamicina, o extrato foi mais efetivo, demonstrando ser um promissor agente antibacteriano.

  3. Termiticidal Activity of Parkia biglobosa (Jacq Benth Seed Extracts on the Termite Coptotermes intermedius Silvestri (Isoptera: Rhinotermitidae

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    Bolarinwa Olugbemi

    2012-01-01

    Full Text Available The chemical and mineral composition of raw and boiled seeds of the African locust bean, Parkia biglobosa (Jacq Benth, was determined while the termiticidal action of the aqueous, alcoholic, and acetone extracts of the bean seeds were investigated. Variations in the proximate and mineral composition of the raw and boiled seeds were obtained while heavy minerals such as cadmium, cobalt, lead, nickel, and copper had been leached out of the seed during the process of boiling. Extracts from the raw seeds exhibited varying degree of termiticidal activity, while extracts from the boiled seed had no effect on the workers of Coptotermes intermedius Silvestri. Alcoholic extracts were more active than the aqueous and acetone extracts. Termites die within 30 min, 40 min, and 110 min when exposed to concentration of 4 g mL−1 treatments of alcoholic, aqueous, and acetone extracts, respectively.

  4. Análise morfológica e fitoquímica da fava d anta (Dimorphandra mollis Benth.)

    OpenAIRE

    2007-01-01

    O Brasil possui a flora arbórea mais diversificada do mundo, no entanto, a falta de diretrizes técnicas e de conscientização ecológica na sua exploração tem acarretado prejuízos ambientais irreparáveis. Com a expansão da fronteira agrícola nas regiões de Cerrado muitas espécies arbóreas encontram-se ameaçadas de extinção, entre elas, Dimorphandra mollis Benth., planta medicinal conhecida popularmente como fava d anta. Sua importância fármaco-agronômica é devido à presença nos frutos de flavon...

  5. Modulation of the Antibiotic Activity by Extracts from Amburana cearensis A. C. Smith and Anadenanthera macrocarpa (Benth. Brenan

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    Fernando G. Figueredo

    2013-01-01

    Full Text Available The aim of this study was to verify the possible interactions between ethanol extracts of Amburana cearensis A. C. Smith and Anadenanthera macrocarpa (Benth. Brenan, combined with six antimicrobial drugs against multiresistant strains of Staphylococcus aureus and Escherichia coli isolated from humans. The antibacterial activity of the extracts was determined using the minimum inhibitory concentration (MIC. The microdilution assay was performed to verify the interactions between the natural products and the antibiotics using a subinhibitory concentration. The activity of amikacin associated with the extract of Anadenanthera macrocarpa against EC 27 was enhanced, demonstrating an MIC reduction from 128 to 4 μg/mL. Among the β-lactams, no potentiation on its activity was observed, with exception to the antagonism of the natural products with ampicillin against S. aureus 358.

  6. Solid-state 13C NMR and molecular modeling studies of acetyl aleuritolic acid obtained from Croton cajucara Benth

    Science.gov (United States)

    da Silva San Gil, Rosane Aguiar; Albuquerque, Magaly Girão; de Alencastro, Ricardo Bicca; da Cunha Pinto, Angelo; do Espírito Santo Gomes, Fabiano; de Castro Dantas, Tereza Neuma; Maciel, Maria Aparecida Medeiros

    2008-08-01

    Solid-state 13C nuclear magnetic resonance ( 13C NMR) with magic-angle spinning (MAS) and with cross-polarization and magic-angle spinning (CP/MAS) spectra, and differential scanning calorimetry (DSC) techniques were used to obtain structural data from a sample of acetyl aleuritolic acid (AAA) extracted from the stem bark of Croton cajucara Benth. (Euphorbiaceae) and recrystallized from acetone. Since solid-state 13C NMR results suggested the presence of more than one molecule in the unitary cell for the AAA, DSC analysis and molecular modeling calculations were used to access this possibility. The absence of phase transition peaks in the DSC spectra and the dimeric models of AAA simulated using the semi-empirical PM3 method are in agreement with that proposal.

  7. [Use of chigo seed (Campsiandra comosa, Benth) in human nutrition. II. Process of non-industrial manufacture of chiga].

    Science.gov (United States)

    Barreiro, J A; Brito, O; Hevia, P; Pérez, C; Orozco, M

    1984-09-01

    A quantitative study of the traditional process for making "chiga" flour was performed. The "chiga" flour is obtained from the seed of the "chigo" (Campsiandra comosa, Benth) and is utilized as a human food in areas of Venezuela in the Orinoco basin, especially in the State of Apure and in the Territorio Federal Amazonas. The block diagram with the description of the traditional process is presented, together with labor and time requirement studies of the different stages of the process. The yields as well as the requirements for raw materials are also discussed. This research work was carried out to study and provide quantitative information that may allow the duplication of the process, in order to improve the efficiency and yield of the product.

  8. Mimosa pudica (L.) assisted green synthesis and photoluminescence studies of Y2O3:Mg2+ nanophosphor for display applications

    Science.gov (United States)

    Venkatachalaiah, KN; Venkataravanappa, M.; Nagabhushana, H.; Basavaraj, R. B.

    2016-09-01

    For the first time green route method was used to synthesize pure and Mg2+(1-11 mol %) doped Y2O3 nanophosphors by using Mimosa pudica leaves extract as a fuel. The final product was well characterized by powder x-ray diffraction (PXRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), diffuse reflectance spectroscopy (DRS) and photoluminescence (PL).The PXRD result shows the formation of single phase, cubic structure of Y2O3 with crystallite sizes ∼25 nm. The SEM results showed porous and agglomerated structures, TEM images showed the crystallite size of the material and was found to be around ∼ 25 nm. PL emission spectra show the blue light emission under the excitation wavelength of 315 nm. The emission peaks of Mg2+were observed at 428 nm, 515 nm and 600 nm corresponding to the transitions of 4F9/2 → 6Hi7/2 (violet), 4F9/2 → 6Hi5/2 (blue), 4F9/2 → 6HJ3/2 (yellow) respectively. The estimated CIE chromaticity co-ordinate was very close to the national television standard committee value of blue emission. CCT was found to be ∼ 6891 K as a result the present phosphor was potential to be used for warm white light emitting display applications.

  9. Phenolic compounds removal from mimosa tannin model water and olive mill wastewater by energy-efficient electrocoagulation process

    Directory of Open Access Journals (Sweden)

    Marijana Kraljić Roković

    2014-12-01

    Full Text Available The objective of this work was to study the influence of NaCl concentration, time, and current density on the removal efficiency of phenolic compounds by electrocoagulation process, as well as to compare the specific energy consumption (SEC of these processes under different experimental conditions. Electrocoagulation was carried out on two different samples of water: model water of mimosa tannin and olive mill wastewater (OMW. Low carbon steel electrodes were used in the experiments. The properties of the treated effluent were determined using UV/Vis spectroscopy and by measuring total organic carbon (TOC. Percentage of removal increased with time, current density, and NaCl concentration. SEC value increased with increased time and current density but it was decreased significantly by NaCl additions (0-29 g L-1. It was found that electro­coagulation treatment of effluents containing phenolic compounds involves complex formation between ferrous/ferric and phenolic compounds present in treated effluent, which has significant impact on the efficiency of the process.

  10. High-quality draft genome sequence of Rhizobium mesoamericanum strain STM6155, a Mimosa pudica microsymbiont from New Caledonia.

    Science.gov (United States)

    Klonowska, Agnieszka; López-López, Aline; Moulin, Lionel; Ardley, Julie; Gollagher, Margaret; Marinova, Dora; Tian, Rui; Huntemann, Marcel; Reddy, T B K; Varghese, Neha; Woyke, Tanja; Markowitz, Victor; Ivanova, Natalia; Seshadri, Rekha; Baeshen, Mohamed N; Baeshen, Nabih A; Kyrpides, Nikos; Reeve, Wayne

    2017-01-01

    Rhizobium mesoamericanum STM6155 (INSCD = ATYY01000000) is an aerobic, motile, Gram-negative, non-spore-forming rod that can exist as a soil saprophyte or as an effective nitrogen fixing microsymbiont of the legume Mimosa pudica L.. STM6155 was isolated in 2009 from a nodule of the trap host M. pudica grown in nickel-rich soil collected near Mont Dore, New Caledonia. R. mesoamericanum STM6155 was selected as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) genome sequencing project. Here we describe the symbiotic properties of R. mesoamericanum STM6155, together with its genome sequence information and annotation. The 6,927,906 bp high-quality draft genome is arranged into 147 scaffolds of 152 contigs containing 6855 protein-coding genes and 71 RNA-only encoding genes. Strain STM6155 forms an ANI clique (ID 2435) with the sequenced R. mesoamericanum strain STM3625, and the nodulation genes are highly conserved in these strains and the type strain of Rhizobium grahamii CCGE501(T). Within the STM6155 genome, we have identified a chr chromate efflux gene cluster of six genes arranged into two putative operons and we postulate that this cluster is important for the survival of STM6155 in ultramafic soils containing high concentrations of chromate.

  11. Suppression of ovalbumin-induced airway inflammatory responses in a mouse model of asthma by Mimosa pudica extract.

    Science.gov (United States)

    Yang, Eun Ju; Lee, Ji-Sook; Yun, Chi-Young; Ryang, Yong Suk; Kim, Jong-Bae; Kim, In Sik

    2011-01-01

    Asthma is an inflammatory airway disease. The pathogenic mechanisms of asthma include the infiltration of leukocytes and release of cytokines. Mimosa pudica (Mp) has been used traditionally for the treatment of insomnia, diarrhea and inflammatory diseases. Although Mp extract has various therapeutic properties, the effect of this extract on asthma has not yet been reported. This study investigated the suppressive effects of Mp extract on asthmatic responses both in vitro and in vivo. Mp extract was acquired from dried and powdered whole plants of M. pudica using 80% ethanol. BALB/c mice were used for the mouse model of asthma induced by ovalbumin. Mp extract significantly inhibited the HMC-1 cell migration induced by stem cell factor and blocked the release of monocyte chemotactic protein-1 (MCP-1) and interleukin-6 (IL-6) in EoL-1 cells. Leukocytosis, eosinophilia and mucus hypersecretion in asthmatic lung were significantly suppressed by Mp extract. The release of ovalbumin-specific IgE in bronchoalveolar lavage fluid and serum was also decreased. Mp extract treatment resulted in no liver cytotoxicity. The Mp extract has inhibitory properties on asthma and may be used as a potent therapeutic agent for allergic lung inflammation. Copyright © 2010 John Wiley & Sons, Ltd.

  12. Water channel activities of Mimosa pudica plasma membrane intrinsic proteins are regulated by direct interaction and phosphorylation.

    Science.gov (United States)

    Temmei, Yusuke; Uchida, Shinichi; Hoshino, Daisuke; Kanzawa, Nobuyuki; Kuwahara, Michio; Sasaki, Sei; Tsuchiya, Takahide

    2005-08-15

    cDNAs encoding aquaporins PIP1;1, PIP2;1, and TIP1;1 were isolated from Mimosa pudica (Mp) cDNA library. MpPIP1;1 exhibited no water channel activity; however, it facilitated the water channel activity of MpPIP2;1 in a phosphorylation-dependent manner. Mutagenesis analysis revealed that Ser-131 of MpPIP1;1 was phosphorylated by PKA and that cooperative regulation of the water channel activity of MpPIP2;1 was regulated by phosphorylation of Ser-131 of MpPIP1;1. Immunoprecipitation analysis revealed that MpPIP1;1 binds directly to MpPIP2;1 in a phosphorylation-independent manner, suggesting that phosphorylation of Ser-131 of MpPIP1;1 is involved in regulation of the structure of the channel complex with MpMIP2;1 and thereby affects water channel activity.

  13. Involvement of respiratory processes in the transient knockout of net CO2 uptake in Mimosa pudica upon heat stimulation.

    Science.gov (United States)

    Lautner, Silke; Stummer, Michaela; Matyssek, Rainer; Fromm, Jörg; Grams, Thorsten E E

    2014-01-01

    Leaf photosynthesis of the sensitive plant Mimosa pudica displays a transient knockout in response to electrical signals induced by heat stimulation. This study aims at clarifying the underlying mechanisms, in particular, the involvement of respiration. To this end, leaf gas exchange and light reactions of photosynthesis were assessed under atmospheric conditions largely eliminating photorespiration by either elevated atmospheric CO2 or lowered O2 concentration (i.e. 2000 μmol mol(-1) or 1%, respectively). In addition, leaf gas exchange was studied in the absence of light. Under darkness, heat stimulation caused a transient increase of respiratory CO2 release simultaneously with stomatal opening, hence reflecting direct involvement of respiratory stimulation in the drop of the net CO2 uptake rate. However, persistence of the transient decline in net CO2 uptake rate under illumination and elevated CO2 or 1% O2 makes it unlikely that photorespiration is the metabolic origin of the respiratory CO2 release. In conclusion, the transient knockout of net CO2 uptake is at least partially attributed to an increased CO2 release through mitochondrial respiration as stimulated by electrical signals. Putative CO2 limitation of Rubisco due to decreased activity of carbonic anhydrase was ruled out as the photosynthesis effect was not prevented by elevated CO2 . © 2013 John Wiley & Sons Ltd.

  14. Mechanosensitivity below Ground: Touch-Sensitive Smell-Producing Roots in the Shy Plant Mimosa pudica1[OPEN

    Science.gov (United States)

    Musah, Rabi A.; Lesiak, Ashton D.; Maron, Max J.; Edwards, David; Fowble, Kristen L.; Long, Michael C.

    2016-01-01

    The roots of the shy plant Mimosa pudica emit a cocktail of small organic and inorganic sulfur compounds and reactive intermediates into the environment, including SO2, methanesulfinic acid, pyruvic acid, lactic acid, ethanesulfinic acid, propanesulfenic acid, 2-aminothiophenol, S-propyl propane 1-thiosulfinate, phenothiazine, and thioformaldehyde, an elusive and highly unstable compound that, to our knowledge, has never before been reported to be emitted by a plant. When soil around the roots is dislodged or when seedling roots are touched, an odor is detected. The perceived odor corresponds to the emission of higher amounts of propanesulfenic acid, 2-aminothiophenol, S-propyl propane 1-thiosulfinate, and phenothiazine. The mechanosensitivity response is selective. Whereas touching the roots with soil or human skin resulted in odor detection, agitating the roots with other materials such as glass did not induce a similar response. Light and electron microscopy studies of the roots revealed the presence of microscopic sac-like root protuberances. Elemental analysis of these projections by energy-dispersive x-ray spectroscopy revealed them to contain higher levels of K+ and Cl− compared with the surrounding tissue. Exposing the protuberances to stimuli that caused odor emission resulted in reductions in the levels of K+ and Cl− in the touched area. The mechanistic implications of the variety of sulfur compounds observed vis-à-vis the pathways for their formation are discussed. PMID:26661932

  15. Rapid hydropassive opening and subsequent active stomatal closure follow heat-induced electrical signals in Mimosa pudica.

    Science.gov (United States)

    Kaiser, Hartmut; Grams, Thorsten E E

    2006-01-01

    In Mimosa pudica L., heat stimulation triggers leaflet folding in local, neighbouring and distant leaves. Stomatal movements were observed microscopically during this folding reaction and electrical potentials, chlorophyll fluorescence, and leaf CO(2)/H(2)O-gas exchange were measured simultaneously. Upon heat stimulation of a neighbouring pinna, epidermal cells depolarized and the stomata began a rapid and pronounced transient opening response, leading to an approximately 2-fold increase of stomatal aperture within 60 s. At the same time, net CO(2) exchange showed a pronounced transient decrease, which was followed by a similar drop in photochemical quantum yield at photosystem (PS) II. Subsequently, CO(2)-gas exchange and photochemical quantum yield recovered and stomata closed partly or completely. The transient and fast stomatal opening response is interpreted as a hydropassive stomatal movement caused by a sudden loss of epidermal turgor. Thus, epidermal cells appear to respond in a similar manner to heat-induced signals as the pulvinar extensor cells. The subsequent closing of the stomata confirms earlier reports that stomatal movements can be induced by electrical signals. The substantial delay (several minutes) of guard cell turgor loss compared with the immediate response of the extensor and epidermal cells suggests a different, less direct mechanism for transmission of the propagating signal to the guard cells.

  16. Effect of Mimosa pudica (Linn.) extract on anxiety behaviour and GABAergic regulation of 5-HT neuronal activity in the mouse.

    Science.gov (United States)

    Ayissi Mbomo, Rigobert; Gartside, Sasha; Ngo Bum, Elizabeth; Njikam, Njifutie; Okello, Ed; McQuade, Richard

    2012-04-01

    Mimosa pudica (Linn.) (M. pudica L.) is a plant used in some countries to treat anxiety and depression. In the present study we investigated the effects of an aqueous extract of M. pudica L. on mouse anxiety-like behaviour using the elevated T maze, and on regulation of dorsal raphe nucleus (DRN) 5-hydroxytryptamine (5-HT) neuronal activity using an in-vitro mouse brain slice preparation. Acute treatment with M. pudica L. extract had an anxiolytic effect on behaviour in the elevated T maze, specifically on inhibitory avoidance behaviour. Acute application of the extract alone had no effect on the activity of DRN 5-HT neurones. However, when co-applied with the GABA(A) receptor agonist THIP (4,5,6,7-tetrahydroisoxazolo[5,4-c]pyridin-3-ol), the extract enhanced the inhibitory effect of the THIP on DRN 5-HT neurones. These observed effects of M. pudica L. on both behaviour and GABA modulation of 5-HT neuronal activity are similar to the effects of diazepam, the established anxiolytic and positive modulator of the GABA(A) receptor. This study suggests that the aqueous extract of M. pudica L. contains a positive modulator of GABA(A) receptor function and provides impetus for further investigation of the neuropharmacologically active constituents of the extract.

  17. Lesões de pele causadas pelos espinhos de Mimosa pudica (Leg. Mimosoideae nos membros de bovinos e ovinos no estado do Pará Cutaneous lesions of the legs caused by the thorns of Mimosa pudica (Leg. Mimosoideae in cattle and sheep in the State of Pará

    Directory of Open Access Journals (Sweden)

    José Diomedes Barbosa

    2009-05-01

    Full Text Available Foi estudada uma doença em bovinos e ovinos caracterizada por lesões ulcerativas e granulomatosas da pele dos membros. Os estudos epidemiológicos e patológicos permitiram concluir que essas lesões são causadas pelos espinhos de Mimosa pudica (Leg. Mimosoideae. A doença foi observada somente em pastagens acentuadamente infestadas e os animais se recuperaram rapidamente após retirados destes pastos. Nos ovinos as lesões atingiam partes mais altas dos membros do que nos bovinos, devido ao seu menor porte.A condition of cattle and sheep characterized by ulcerous and granulomatous skin lesions of the legs was studied. Epidemiological data and the nature of the lesions indicate that they are caused by the thorns of Mimosa pudica (Leg. Mimosoideae. The disease was observed only in pastures largely infested by the plant, and animals recover quickly when moved from them.

  18. Composition chimique et activité antimicrobienne des huiles essentielles de Thymus algeriensis Boiss. & Reut. et Thymus ciliatus (Desf. Benth. du Maroc

    Directory of Open Access Journals (Sweden)

    Chaouch, A.

    2010-01-01

    Full Text Available Chemical composition and antimicrobial activity of the Thymus algeriensis Boiss. & Reut. and Thymus ciliatus (Desf. Benth. essential oils of Morocco. The aim of this work is to study the chemical composition and antibacterial and antifungal activity of essential oils of Thymus algeriensis Boiss. & Reut. and Thymus ciliatus (Desf. Benth. of Morocco against seven microorganisms. The essential oils of T. ciliatus are characterized by the presence of thymol (44.2%, β-E-ocimene (25.8% and α-terpinene (12.3% as principal chemical components. The essential oils of T. algeriensis are formed mainly by camphor (27.7% and α-pinene (20.5%. The oil of T. ciliatus showed a strong antibacterial and antifungal activity against all tested bacteria and fungi. This bioactivity is due mainly to the richness of this essential oil in thymol known for its effectiveness against the microbial agents.

  19. Isolasi Dan Analisis Komponen Kimia Minyak Atsiri Dari Daun Jinten (Coleus Aromatikus Benth) Dengan GC – MS Dan Uji Anti Bakteri

    OpenAIRE

    Sinulingga, Bagus

    2011-01-01

    The research was conducted whit the scale of laboratore. The object ofthis research was fresh leave jinten. The treatment done was to isolatethe essential oil of leave jinten (Coleus Aromaticus Benth) though water distillation . To analyzed chemical components was done with mass Spectrometry – gas Cromatography. The reuslt of gas cromatography showed 15 apexes meaning that the essensial oil of the leave jinten. Based on analysis with spectrometry mass inducted that chemical compound in...

  20. Isolasi Dan Analisis Komponen Kimia Minyak Atsiri Dari Daun Jinten (Coleus Aromatikus Benth) Dengan GC – MS Dan Uji Anti Bakteri

    OpenAIRE

    Sinulingga, Bagus

    2011-01-01

    The research was conducted whit the scale of laboratore. The object ofthis research was fresh leave jinten. The treatment done was to isolatethe essential oil of leave jinten (Coleus Aromaticus Benth) though water distillation . To analyzed chemical components was done with mass Spectrometry – gas Cromatography. The reuslt of gas cromatography showed 15 apexes meaning that the essensial oil of the leave jinten. Based on analysis with spectrometry mass inducted that chemical compound in...

  1. Germinação de sementes de plantas daninhas de pastagens cultivadas: Mimosa pudica e Ipomoea asarifolia Seed germination of weeds from cultivated pasture areas: Mimosa pudica and Ipomoea asarifolia

    Directory of Open Access Journals (Sweden)

    A.P.S. Souza Filho

    2001-04-01

    Full Text Available Malícia (Mimosa pudica e salsa (Ipomoea asarifolia são importantes plantas daninhas que infestam áreas de pastagens cultivadas da região amazônica brasileira. Neste trabalho foram analisados os efeitos de fatores relacionados ao solo e clima na variação da germinação (percentual e índice de velocidade de germinação - IVG de sementes dessas espécies. A germinação foi monitorada em um período de 15 dias, com contagens diárias e eliminação das sementes germinadas. Os resultados mostraram que os fatores de solo alumínio, pH, cálcio e magnésio não influenciam a germinação das duas espécies de plantas daninhas. A germinação das sementes de malícia e salsa pode ocorrer tanto na presença como na ausência de luz. A temperatura ótima de germinação (percentual e IVG para ambas as espécies foi de 30 ºC contínua e 25-35 ºC alternada, embora as sementes de malícia tenham germinado satisfatoriamente em temperaturas contínuas superiores e inferiores a 30 ºC e em todas as combinações de temperaturas alternadas. Comparativamente, o índice de velocidade de germinação (IVG foi mais sensível aos efeitos da temperatura do que o percentual de germinação. Ambas as espécies responderam negativamente ao aumento da salinidade, sendo a espécie malícia mais tolerante ao sal do que a salsa.Mimosa pudica and Ipomoea asarifolia are important weeds of cultivated pasture in the Brazilian Amazon Region. This research analyzes the effects of the change in soil and climate factors on the germination (percentage and germination speed index of these species. Germination was monitored for a 15-day period, with daily counting and elimination of the germinated seeds. The results showed that soil factors such as aluminum, pH, calcium, and magnesium did not influence the germination of the two weed species. The germination of both M. pudica and I. asarifolia is independent of light exposure. Optimum germination (percentage and

  2. Estudo fitoquímico e avaliação da atividade anticolinesterásica de extratos da casca da raiz da Mimosa tenuiflora (Willd.) Poiret

    OpenAIRE

    Neves, Maiane dos Santos

    2015-01-01

    Mimosa tenuiflora (Willd.) Poiret (jurema preta) é uma espécie da família Fabaceae nativa da Caatinga, encontrada largamente em todo nordeste brasileiro. Na medicina popular, as cascas do caule e da raiz da jurema preta são utilizadas no tratamento de diversas enfermidades, como queimaduras e inflamações, além de ser empregada tradicionalmente como bebida em rituais indígenas. Com relação à casca da raiz, há poucos estudos descritos na literatura sobre seu perfil químico e propriedades biológ...

  3. Leaf Anatomic Structure of Mimosa Bimucronata%簕仔树的叶片解剖结构研究

    Institute of Scientific and Technical Information of China (English)

    黄丽宜; 缪绅裕; 陈健辉; 李宝钥; 李里

    2011-01-01

    利用石蜡切片技术对筋仔树(Mimosa bimucronata)叶片进行解剖研究.结果表明,筋仔树的叶是异面叶,表皮角质层的角质化程度随发育的进行而增大.叶肉细胞排列紧密,在海绵组织和栅栏组织之间具有贮藏组织.叶脉维管组织发达,具四级叶脉组成脉序,第一级、第二级叶脉类型属半周韧、有限维管束;第三级、第四级叶脉类型属外韧、有限维管束.成熟叶片维管束鞘中有晶体细胞存在.另外,PAS反应显示,在叶片发育初期,海绵组织薄壁细胞、维管束鞘细胞、贮藏组织细胞中有较多淀粉粒分布,以后随着叶片的发育,这些部位细胞淀粉粒的分布越来越少.作为入侵植物,簕仔树叶片结构中角质层的厚度、淀粉粒的分布、栅栏组织与海绵组织紧密的胞间隙与其适合在耐热、耐涝、耐旱的环境生长相适应.%The leaf structure of Mimosa bimucronata is studied by paraffin method under the light microscope. The results show that the leaf of M. Bimucronata is dorsi-ventral leaf,and that its cuticle becomes thicker as it grows up. The mesophyll cells are arranged closely and there is storage tissue between palisade tissue and spongy tissue. The,vascular tissues of the vein are well-developed and the venation is formed by four veins:the primary and secondary veins are semi-amphicribral,and closed vascular bundles while the tertiary and quaternary veins are collateral,and closed vascular bundles. Crystal cells exist in the vascular bundle sheath of the mature leaf. In addition, the PAS staining shows that in the developmental stage of the leaf,a lot of starch grains are distributed within the parenchyma cells of the spongy tissue, the vascular bundle sheath cells and the storage tissue cells. These starch grains reduce gradually following the development of the leaf. As an invasive plant, the M. Bimucronata has adapted the thickness of its cuticle, the distribution pattern of its starch

  4. Prosopis laevigata and Mimosa biuncifera (Leguminosae, jointly influence plant diversity and soil fertility of a Mexican semiarid ecosystem

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    Rosalva García-Sánchez

    2012-03-01

    Full Text Available Prosopis laevigata and Mimosa biuncifera are frequently found in arid and semiarid shrublands, but scarce information is available about their influence on plant community structure and soil fertility. We compared plant community structure, diversity and soil nutrients of three semiarid shrubland sites located in Mezquital Valley, Mexico. These sites differ in their dominant species: Site 1 (Bingu P. laevigata, Site 2 (González M. biuncifera, and Site 3 (Rincón with the presence of both legumes. The results showed that the plant community with P. laevigata and M. biuncifera (Site 3 had more cover, taller plants and higher plant diversity than sites with only one legume (Site 1 and Site 2. Soil organic matter (SOM, soil organic carbon (SOC, total nitrogen (TN, phosphorus-Olsen (P and C mineralization were higher in the soil under the canopy of both legumes than in bare soil. In contrast, soil cation concentrations were lower under the canopy of P. laevigata, but not for M. biuncifera. In addition, the density of arbuscular mycorrhizal fungi spores was higher within the soil under the canopy of M. biuncifera than in the soil under the canopy of P. laevigata. Thus, resource islands (RI created by P. laevigata increased the amounts of SOC, TN and P when compared with the RI of M. biuncifera. This study provided evidences about the importance of species identity in order to expand the niche availability for the establishment of other plants, and highlights that P. laevigata and M. biuncifera jointly influencing plant colonization within semiarid ecosystemsProsopis laevigata y Mimosa biuncifera coexisten en los matorrales semiáridos; sin embargo, se desconoce su influencia sobre la diversidad de la comunidad vegetal y el suelo. Este estudio evaluó el efecto de P. laevigata y M. biuncifera sobre la estructura, diversidad vegetal y nutrimentos del suelo, en tres matorrales del Valle del Mezquital, México. Los sitios difieren en la especie dominante

  5. ESTRUTURA E DISTRIBUIÇÃO ESPACIAL DE Mimosa tenuiflora (Willd. Poir. EM DOIS FRAGMENTOS DE CAATINGA EM PERNAMBUCO

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    JOÃO TAVARES CALIXTO JÚNIOR

    2011-01-01

    Full Text Available Mimosa tenuiflora (Willd. (jurema preta is a xerophytic species of the shrub-arboreal habit, indicative of secondary succession stage of recovery or progressive, widely disseminated by different caatinga physiognomies of northeastern Brazil, bringing great potential. For the few studies that address their environmental performance, this study aimed to investigate structural and ecological aspects of its population, based data in diameter and height, and characterize the spatial distribution pattern of this species in the study areas. The phytosociological survey was conducted in two areas with 2 and 80 hectares of caatinga at the Experimental Station of Embrapa Semiarid, Petrolina - PE. 10 units were plotted on a random sample measuring 8.0 x 40m, and included all individuals living in diameter at soil level K 3 cm, and measured the total height of the same. Were sampled 283 and 61 individuals in 100 and 80% of plots in areas I and II respectively, inserted mostly in the first diameter classes (3.0 - 6.0 cm and 6.1 - 9.0 cm and height (2.0 - 3.0 m and 3.1 - 4 m, without trend to standard reverse-J-shape in areas. The spatial distribution, measured by the Payandeh index, reached a value of 2.4 in area I and 7.8 in area II, indicating that the species has a aggregated distribution in both areas. The calculated values for the of Liocourt quotient in areas I and II respectively, got an average of 0.67 and 1.12 with great variation, showing unbalance, despite being in progressive process of natural regeneration.

  6. Prosopis laevigata and Mimosa biuncifera (Leguminosae, jointly influence plant diversity and soil fertility of a Mexican semiarid ecosystem

    Directory of Open Access Journals (Sweden)

    Rosalva García-Sánchez

    2012-03-01

    Full Text Available Prosopis laevigata and Mimosa biuncifera are frequently found in arid and semiarid shrublands, but scarce information is available about their influence on plant community structure and soil fertility. We compared plant community structure, diversity and soil nutrients of three semiarid shrubland sites located in Mezquital Valley, Mexico. These sites differ in their dominant species: Site 1 (Bingu P. laevigata, Site 2 (González M. biuncifera, and Site 3 (Rincón with the presence of both legumes. The results showed that the plant community with P. laevigata and M. biuncifera (Site 3 had more cover, taller plants and higher plant diversity than sites with only one legume (Site 1 and Site 2. Soil organic matter (SOM, soil organic carbon (SOC, total nitrogen (TN, phosphorus-Olsen (P and C mineralization were higher in the soil under the canopy of both legumes than in bare soil. In contrast, soil cation concentrations were lower under the canopy of P. laevigata, but not for M. biuncifera. In addition, the density of arbuscular mycorrhizal fungi spores was higher within the soil under the canopy of M. biuncifera than in the soil under the canopy of P. laevigata. Thus, resource islands (RI created by P. laevigata increased the amounts of SOC, TN and P when compared with the RI of M. biuncifera. This study provided evidences about the importance of species identity in order to expand the niche availability for the establishment of other plants, and highlights that P. laevigata and M. biuncifera jointly influencing plant colonization within semiarid ecosystems

  7. Prosopis laevigata and Mimosa biuncifera (Leguminosae), jointly influence plant diversity and soil fertility of a Mexican semiarid ecosystem.

    Science.gov (United States)

    García-Sánchez, Rosalva; Camargo-Ricalde, Sara Lucía; García-Moya, Edmundo; Luna-Cavazos, Mario; Romero-Manzanares, Angélica; Montaño, Noé Manuel

    2012-03-01

    Prosopis laevigata and Mimosa biuncifera are frequently found in arid and semiarid shrublands, but scarce information is available about their influence on plant community structure and soil fertility. We compared plant community structure, diversity and soil nutrients of three semiarid shrubland sites located in Mezquital Valley, Mexico. These sites differ in their dominant species: Site 1 (Bingu) P. laevigata, Site 2 (González) M. biuncifera, and Site 3 (Rincón) with the presence of both legumes. The results showed that the plant community with P. laevigata and M. biuncifera (Site 3) had more cover, taller plants and higher plant diversity than sites with only one legume (Site 1 and Site 2). Soil organic matter (SOM), soil organic carbon (SOC), total nitrogen (TN), phosphorus-Olsen (P) and C mineralization were higher in the soil under the canopy of both legumes than in bare soil. In contrast, soil cation concentrations were lower under the canopy of P. laevigata, but not for M. biuncifera. In addition, the density of arbuscular mycorrhizal fungi spores was higher within the soil under the canopy of M. biuncifera than in the soil under the canopy of P. laevigata. Thus, resource islands (RI) created by P. laevigata increased the amounts of SOC, TN and P when compared with the RI of M. biuncifera. This study provided evidences about the importance of species identity in order to expand the niche availability for the establishment of other plants, and highlights that P. laevigata and M. biuncifera jointly influencing plant colonization within semiarid ecosystems.

  8. Purification and Biochemical Characterization of a Novel Ecto-Apyrase, MP67, from Mimosa pudica1[C][W][OA

    Science.gov (United States)

    Okuhata, Riku; Takishima, Takeshi; Nishimura, Naoaki; Ueda, Shogo; Tsuchiya, Takahide; Kanzawa, Nobuyuki

    2011-01-01

    We have previously reported the presence of an apyrase in Mimosa pudica. However, only limited information is available for this enzyme. Thus, in this study, the apyrase was purified to homogeneity. The purified enzyme had a molecular mass of around 67 kD and was able to hydrolyze both nucleotide triphosphate and nucleotide diphosphate as substrates. The ratio of ATP to ADP hydrolysis velocity of the purified protein was 0.01 in the presence of calcium ion, showing extremely high substrate specificity toward ADP. Thus, we designated this novel apyrase as MP67. A cDNA clone of MP67 was obtained using primers designed from the amino acid sequence of trypsin-digested fragments of the protein. In addition, rapid amplification of cDNA ends-polymerase chain reaction was performed to clone a conventional apyrase (MpAPY2). Comparison of the deduced amino acid sequences showed that MP67 is similar to ecto-apyrases; however, it was distinct from conventional apyrase based on phylogenetic classification. MP67 and MpAPY2 were expressed in Escherichia coli, and the recombinant proteins were purified. The recombinant MP67 showed high substrate specificity toward ADP rather than ATP. A polyclonal antibody raised against the recombinant MP67 was used to examine the tissue distribution and localization of native MP67 in the plant. The results showed that MP67 was ubiquitously distributed in various tissues, most abundantly in leaves, and was localized to plasma membranes. Thus, MP67 is a novel ecto-apyrase with extremely high substrate specificity for ADP. PMID:21788364

  9. Effect of Mimosa pudica root extract on vaginal estrous and serum hormones for screening of antifertility activity in albino mice.

    Science.gov (United States)

    Ganguly, Mausumi; Devi, Nirada; Mahanta, Rita; Borthakur, Mridul K

    2007-12-01

    Several plants are traditionally used as birth control agents by the rural people in India. Mimosa pudica is one of the folk medicinal plants commonly used as antifertility agent in some places in India. The present work was carried out to evaluate the claimed antifertility effect of the plant by carrying out pharmacological studies with the root extract of the plant. Air-dried roots of M. pudica were extracted using methanol. Dried methanol extract of the root was administered orally to Swiss albino mice for 21 consecutive days. Estrous cycle, reproductive hormones (LH, FSH, prolactin, estradiol and progesterone) and number of litters produced were studied in both control and extract-administered groups by using standard methods. Phytochemical studies of the methanolic root extract were carried out using qualitative and thin-layer chromatography methods. M. pudica root extract, when administered orally at a dose of 300 mg/kg body weight/day, prolonged the length of the estrous cycle with significant increase in the duration of the diestrous phase and reduced the number of litters in albino mice. The number of litters was increased in the posttreatment period. The analysis of the principal hormones (LH, FSH, prolactin, estradiol and progesterone) involved in the regulation of the estrous cycle showed that the root extract altered gonadotropin release and estradiol secretion. The root extract of M. pudica has antifertility effect as it prolongs the estrous cycle and disturbs the secretion of gonadotropin hormones in albino mice. The decrease in FSH level in the proestrus and estrus stages in the extract-administered group compared with those of control animals indicates the disturbance of estrous cycle and ovulation through suppression of FSH.

  10. Protective effect of Mimosa pudica L. in an L-arginine model of acute necrotising pancreatitis in rats.

    Science.gov (United States)

    Kaur, Jagdeep; Sidhu, Shabir; Chopra, Kanwaljit; Khan, M U

    2016-07-01

    Mimosa pudica is used in traditional medicine for treating various disorders such as inflammatory conditions, diarrhoea, insomnia, alopecia, urogenital infections and wounds. The present study investigated the effect of M. pudica extract (MPE) on L-arginine-induced acute necrotising pancreatitis in rats. The ethanolic extract of M. pudica leaves was studied for the presence of quercetin and gallic acid using high-performance liquid chromatography. Four groups were employed-normal control rats, L-arginine control rats (two intraperitoneal [i.p.] injections of 2 g/kg at an interval of 1 h), MPE-treated rats (400 mg/kg orally) and melatonin-treated rats (positive control 10 mg/kg i.p.), which were further divided into subgroups according to time points (24 h, 3 days and 14 days). Serum amylase, lipase, tumour necrosis factor-α (TNF-α), pancreatic amylase, nucleic acid content, protein, transforming growth factor-β1 (TGF-β1), thiobarbituric reactive substances, glutathione, nitrite/nitrate, collagen content and histopathological examination were carried out. MPE significantly improved acute necrotising pancreatitis by modulating diagnostic markers of pancreatitis such as serum lipase and pancreatic amylase, inflammation (TNF-α), and oxidative and nitrosative stress. Moreover, MPE administration induced regenerative changes in the pancreas evidenced by increased levels of pancreatic proteins, nucleic acid content and histopathology report. In addition, MPE improved TGF-β1 and collagen levels thereby preventing fibrosis. The current investigation indicates the novel role of MPE in reducing the severity of acute necrotising pancreatitis by plausible mechanisms such as anti-inflammatory and anti-fibrotic activity and by promoting repair and regeneration of the pancreas.

  11. Metal biosorption capability of Cupriavidus taiwanensis and its effects on heavy metal removal by nodulated Mimosa pudica.

    Science.gov (United States)

    Chen, Wen-Ming; Wu, Chih-Hui; James, Euan K; Chang, Jo-Shu

    2008-03-01

    A novel metal biosorption system consisting of the symbiotic combination of an indigenous metal-resistant rhizobial strain, Cupriavidus taiwanensis TJ208, and its host plant Mimosa pudica has been developed for the removal of heavy-metal pollutants. Free-living C. taiwanensis TJ208 cells were able to adsorb 50.1, 19.0, and 19.6 mg/g of Pb, Cu, and Cd, respectively. After nodulation via inoculation with strain TJ208, the metal uptake ability of M. pudica markedly increased, as the nodulated M. pudica displayed a high metal uptake capacity (qmax) of 485, 25, and 43 mg/g, respectively, which is 86, 12, and 70% higher than that of nodule-free plants. Moreover, with TJ208 nodules, the M. pudica plant also displayed a 71, 81, and 33% enhancement in metal adsorption efficiency (eta) for Pb, Cu, and Cd, respectively. The nodulation appeared to give the greatest enhancing effect on the uptake of Pb, which is consistent with the preference of metal adsorption ability of TJ208. This seems to indicate the crucial role that the rhizobial strain may play in stimulating metal uptake of the nodulated plant. Furthermore, the results show that metal accumulation in the nodulated plant mainly occurred in the roots, accounting for 65-95% of total metal uptake. In contrast, the nodules and the shoots only contributed to 3-12 and 2-23% of total metal uptake, respectively. Nevertheless, the specific adsorption capacity of nodules is comparable to that of the roots. Hence, this work demonstrates the feasibility and effectiveness of using the nodulated plants to promote phyto-removal of heavy metals from the polluted environment as well as to restrict the metal contaminants in the unharmful region of the plant.

  12. Pharmacognostic Study of Mimosa pudica L.%民族药"含羞草"的生药学研究

    Institute of Scientific and Technical Information of China (English)

    谢建中; 邝立华; 游春

    2010-01-01

    目的:为研究和开发豆科含羞草属植物含羞草Mimosa pudica L.提供理论依据.方法:采用性状鉴别、显微鉴别、理化鉴别的方法对含羞草进行生药学研究.结果:含羞草根横切面主要特征为①木栓细胞3~7列,外被落皮层;②皮层宽窄不一,纤维散在,薄壁细胞中含有淀粉粒或色素块,油细胞偶见,在皮层、韧皮部可见分泌腔,内含有不规则黄色分泌物;③韧皮部纤维束较多,周围薄壁细胞中含有草酸钙方晶,形成晶鞘;④形成层呈环状,波状弯曲,细胞2~3列;⑤木质部由导管、木薄壁细胞、纤维构成,纤维具有晶鞘;⑥射线细胞1~3列,内含淀粉粒或色素物质.理化实验表明:含羞草含生物碱、有机酸、黄酮类、酚类、氨基酸及内酯性物质等成分.另含含羞草碱(Mimosine)、含羞草甙(mimoside)、D-松醇(D-pinitol).结论:含羞草是一味具有开发前景的民族药材,该研究为其进一步深入研究提供了生药学资料.

  13. Evaluation of the immunotoxicological effects of Dimorphandra mollis Benth., Fabaceae, in rats Avaliação dos efetos imunotoxicológicos de Dimorphandra mollis Benth., Fabaceae, em ratos

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    Cássia A. O. Feres

    2010-09-01

    Full Text Available Dimorphandra mollis Benth., Fabaceae, also known as "faveira" or "fava-d'anta", is a plant common to the central woodsy meadow region of Brazil. It is well known for its antioxidant, antiplatelet and, principally, vasoprotective properties. Its principal component is rutin. The objective of this study is the evaluation of the safety of the use of the dried D. mollis extract in rodents. The rutin content of the standardized extract was 76.0±3%. With respect to the biochemical and hematological parameters evaluated, no alterations in the groups of rats that received 1000 and 2000 mg/kg doses of D. mollis were observed, but an increase in eosinophiles occurred. Hyperactivity of the white splenic pulp was detected in the group that received the 2000 mg/kg dose of D. mollis. In the evaluation of the lymphproliferative response with 1000 and 2000 mg/kg, no alterations were observed, and a decrease in IgG was only observed in the studies with a 2000 mg/kg dose. The results obtained with rodents suggest that no toxicity exists with the administration of dried D. mollis extract in a 1000 mg/kg dose.A Dimorphandra mollis Benth., Fabaceae, conhecida como faveira ou fava-d'anta, é uma planta comum do cerrado central do Brasil, muito utilizada por suas propriedades antioxidante, antiplaquetária e, principalmente, como vasoprotetora. Seu principal marcador é a rutina. Este estudo teve como objetivo avaliar a segurança da utilização do extrato seco de D. mollis em roedores. O extrato foi extraído, padronizado e quantificado apresentando teor de 76,0±3% de rutina. Nos parâmetros bioquímicos e hematológicos avaliados, não se observou alterações nos grupos de machos e fêmeas que receberam a dose de 1000 e 2000 mg/kg de D. mollis, mas observou-se um aumento de eosinófilos. Nos estudos histopatológicos detectou-se hiperreatividade da polpa branca esplênica, no grupo que recebeu a dose de 2000 mg/kg de D. mollis. Na avaliação da resposta

  14. Nitric oxide mediates the fungal elicitor-induced puerarin biosynthesis in Pueraria thomsonii Benth. suspension cells through a salicylic acid (SA)-dependent and a jasmonic acid (JA)-dependent signal pathway

    Institute of Scientific and Technical Information of China (English)

    XU Maojun; DONG Jufang; ZHU Muyuan

    2006-01-01

    Nitric oxide (NO) has emerged as a key signaling molecule in plant secondary metabolite biosynthesis recently. In order to investigate the molecular basis of NO signaling in elicitor-induced secondary metabolite biosynthesis of plant cells, we determined the contents of NO, salicylic acid (SA), jasmonic acid (JA), and puerarin in Pueraria thomsonii Benth. suspension cells treated with the elicitors prepared from cell walls of Penicillium citrinum. The results showed that the fungal elicitor induced NO burst, SA accumulation and puerarin production of P. thomsonii Benth. cells. The elicitor-induced SA accumulation and puerarin production was suppressed by nitric oxide specific scavenger cPITO, indicating that NO was essential for elicitor-induced SA and puerarin biosynthesis in P. thomsonii Benth. cells. In transgenic NahG P. thomsonii Benth. cells, the fungal elicitor also induced puerarin biosynthesis, NO burst, and JA accumulation, though the SA biosynthesis was impaired. The elicitor-induced JA accumulation in transgenic cells was blocked by cPITO, which suggested that JA acted downstream of NO and its biosynthesis was controlled by NO. External application of NO via its donor sodium nitroprusside (SNP) enhanced puerarin biosynthesis in transgenic NahG P. thomsonii Benth. cells, and the NO-triggered puerarin biosynthesis was suppressed by JA inhibitors IBU and NDGA, which indicated that NO induced puerarin production through a JA-dependent signal pathway in the transgenic cells. Exogenous application of SA suppressed the elicitor-induced JA biosynthesis and reversed the inhibition of IBU and NDGA on elicitor-induced puerarin accumulation in transgenic cells, which indicated that SA inhibited JA biosynthesis in the cells and that SA might be used as a substitute for JA to mediate the elicitor- and NO-induced puerarin biosynthesis. It was, therefore, concluded that NO might mediate the elicitor-induced puerarin biosynthesis through SA- and JA-dependent signal

  15. Study on Characters of Hard Seeds of Amphicarpaea edgeworthii Benth. (Leguminosae)and Methods for Breaking Dormancy%Study on Characters of Hard Seeds of Amphicarpaea edgeworthii Benth. (Leguminosae)and Methods for Breaking Dormancy

    Institute of Scientific and Technical Information of China (English)

    Lifeng ZHANG; Xingwen WU; Yuehui SHE

    2012-01-01

    [Objective] This study aimed to understand the characters of hard seeds of Amphicarpaea edgeworthii Benth. (Leguminosae) and explore the methods for break- ing dormancy. [Method] For both aerial and subterranean seeds of A. edgeworthii, the morphological characteristics were observed and the appropriate temperature for germination was explored. For aerial seeds of A. edgeworthii, the characters of hard seeds were studied, and concentrated sulfuric acid treatment, hot water soak treat- ment and mechanical damage treatment were compared to explore the methods for breaking seed dormancy. [Result] The aerial seeds were oblate and averaged 3.38 mm in length, 3.02 mm in width, 1.88 mm in thickness, 15.32 g in thousand grain weight, with a hard seed rate after of up to 98% natural maturation. The subter- ranean seeds were approximately oblate with a maximum diameter of 15 mm and a hundred grain weight of (50.08-58.26 g); among all the treatments for breaking hardseededness, cutting seed coat treatment and concentrated sulfuric acid treatment for 20 minutes were the most effective methods, whereas hot water soak treatment was the least effective method; constant temperature between 20 and 30 ℃ was optimum for the germination of aerial seeds, and alternative temperature of 30/20 ℃ was most appropriate for the germination of subterranean seeds. [Conclusion] Cutting seed coat treatment and concentrated sulfuric acid treatment for 20 minutes were the most effective methods to break the hardseededness of A. edgeworthii.

  16. Espécies potenciais para recuperação de áreas de preservação permanente no Planalto Catarinense

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    Paula Iaschitzki Ferreira

    Full Text Available Com o objetivo de selecionar espécies para programas de recuperação em Áreas de Preservação Permanente no Planalto Catarinense foi realizado o levantamento da composição florística e da estrutura fitossociológica e, a caracterização dos grupos ecológicos e síndromes de dispersão das espécies ocorrentes nestes locais. A área de estudo, fazenda Campo de Dentro, localiza-se no município de Otacílio Costa (SC, onde foi empregado o método de quadrantes, em 20 transecções compostas por 20 pontos amostrais. As espécies que apresentaram os maiores valores de importância, potenciais para recuperação, foram Sebastiania commersoniana (Baill. L.B.Sm. & Downs; Mimosa scabrella Benth.; Cinnamomum amoenum (Nees & Mart. Kosterm.; Lithraea brasiliensis Marchand, Ocotea pulchella (Nees & Mart. Mez; Ilex paraguariensis A.St.-Hil.; Matayba elaeagnoides Radlk.; Ilex theezans Mart. exReissek e Vernonanthura discolor (Spreng. H.Rob., que representam os diferentes grupos ecológicos, destacando-se a zoocoria como síndrome de dispersão. A alta diversidade de espécies arbóreas evidencia a riqueza desses locais que, muitas vezes, são negligenciados em projetos de recuperação.

  17. DISTRIBUIÇÃO DAS POSTURAS, DE LARVAS E DE INIMIGOS NATURAIS DE Oncideres impluviata (Bermar, 1824 (COL.; CERAMBYCIDAE.

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    José H. Pedrosa-Macedo

    1992-12-01

    Full Text Available Com o objetivo de verificar a distribuição, independente do efeito de cada componente, contou-se posturas, larvas sadias, predadas ou parasitadas de Oncideres impluviata em galhos de bracatinga (Mimosa scabrella Benth.. O levantamento foi realizado no período de 1984/85, em Almirante Tamandaré, Paraná. Para o estudo da distribuição das larvas e de imimigos naturais foi medida a distância da base do galho até o ponto onde se encontrava cada larva sadia ou parasitada, galeria vazia ou predador. Os resultados demostraram que as larvas se concentram a partir dos 10,0 cm do ponto base do galho, estendendo-se até 40,0 cm, com uma concentração máxima entre 10,0 e 20,0 cm. Os predadores e parasitas apresentam uma concentração não coincidente, sendo que os parasitas concentram-se entre 20, e 30,0 cm e os predadores entre 10,0 e 20,0 cm a partir da base. Pelos resultados obtidos conclui-se que as larvas se estabelecem numa posição onde encontram as melhores condições de sobrevivência, ao passo que os parasitas e predadores estão na depend~encia das condições intrinsecas de cada espécie em função das condições ideais da fase de desenvolvimento dos hospedeiros ou presas.

  18. Eficiência e período de arividade dos inimigos naturais de Oncideres impluviata (Fermar, 1824(Col.: Cerambycidae.

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    Ervandil Corrêa Costa

    2009-09-01

    Full Text Available Normal 0 21 false false false MicrosoftInternetExplorer4 Objetivou-se, com este estudo, o conhecimento do conjunto de inimigos naturais e a ação destes ao longo do desenvolvimento das larvas de Oncideres impluviata. No período de 1984/85, em Almirante Tamandaré – Paraná, coletou-se periodicamente galhos de bracatinga (Mimosa scabrella Benth. cortados pelo serrador. As larvas coletadas a campo completaram o ciclo em laboratório em tubos de ensaio contendo serragem umedecida de bracatinga. Ao retirar-se as larvas dos galhos anotou-se o ponto em relação à base-ápice do galho, além da data de coleta e o instar em que se encontravam. Avaliou-se a eficiência dos inimigos naturais e o período de atividade destes. As observações efetuadas permitiram concluir que a eficiência dos inimigos naturais e o período de atividade destes. As observações efetuadas permitiram concluir que a ação predatória foi mais eficiente do que o parasitismo, sendo que esta tende a diminuir com o desenvolvimento larval, ao passo que a ação predatória aumenta concomitantemente no decorrer do desenvolvimento das larvas. O parasitóide mais freqüente foi Cenocoelius sp. (Hym.; Gracanidae e entre os predadores destacam-se Cregya difformis (Col.; Coleridae e Lithrips sp. (Thys.; Phloeothripidae, entre outros.

  19. Scopolin isolated from Erycibe obtusifolia Benth stems suppresses adjuvant-induced rat arthritis by inhibiting inflammation and angiogenesis.

    Science.gov (United States)

    Pan, Rong; Dai, Yue; Gao, Xinghua; Xia, Yufeng

    2009-07-01

    Despite scopolin is a main coumarin constituent in the stems of Erycibe obtusifolia Benth, a herb drug that has long been utilized in traditional Chinese medicine for the treatment of rheumatoid arthritis, little information is available about the pharmacological activities of this compound. The present study was performed to investigate the anti-rheumatic effects of scopolin in adjuvant-induced arthritis (AIA) in rats, and explore the underlying mechanisms of action in views of anti-inflammatory and anti-angiogenic properties in the synovial tissues. Scopolin (50, 100 mg/kg), injected intraperitoneally for 10 days from the onset of secondary response, significantly inhibited both inoculated and non-inoculated paw swelling as well as articular index scores in AIA. Meanwhile, the mean body weight of rats treated with scopolin was higher than that of model group. Rats treated with high dose of scopolin (100 mg/kg) preserved a nearly normal histological architecture of the joints and showed a significant reduction of the new blood vessels in the synovial tissues. Additionally, scopolin could reduce IL-6, VEGF and FGF-2 expressions in rat synovial tissues. In conclusion, scopolin can reduce the clinical symptoms of rat AIA by inhibiting inflammation and angiogenesis, and this compound may be a potent agent for angiogenesis related diseases and can serve as a structural base for screening more potent synthetic analogs.

  20. Impact of Organic and Inorganic Fertilizers Application on the Phytochemical and Antioxidant Activity of Kacip Fatimah (Labisia pumila Benth

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    Ehsan Karimi

    2013-09-01

    Full Text Available A study was conducted to compare secondary metabolites and antioxidant activity of Labisia pumila Benth (Kacip Fatimah in response to two sources of fertilizer [i.e., organic (chicken dung; 10% N:10% P2O5:10% K2O and inorganic fertilizer (NPK green; 15% N, 15% P2O5, 15% K2O] under different N rates of 0, 90, 180 and 270 kg N/ha. The experiment was arranged in a randomized complete block design replicated three times. At the end of 15 weeks, it was observed that the application of organic fertilizer enhanced the production of total phenolics, flavonoids, ascorbic acid, saponin and gluthathione content in L. pumila, compared to the use of inorganic fertilizer. The nitrate content was also reduced under organic fertilization. The application of nitrogen at 90 kg N/ha improved the production of secondary metabolites in Labisia pumila. Higher rates in excess of 90 kg N/ha reduced the level of secondary metabolites and antioxidant activity of this herb. The DPPH and FRAP activity was also highest at 90 kg N/ha. The results indicated that the use of chicken dung can enhance the production of secondary metabolites and improve antioxidant activity of this herb.

  1. POTENCIAL FITOTÓXICO DE Pterodon polygalaeflorus BENTH (LEGUMINOSAE SOBRE Acanthospermum australe (LOEFL. O. KUNTZE E Senna occidentalis (L. LINK

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    VALDENIR JOSÉ BELINELO

    2009-01-01

    Full Text Available The objectives of this research were synthesize and characterize the allelopatic activity of 6a,7bdi-hydroxyvouacapan-17b-oic acid derivatives, isolated from seeds of Pterodon polygalaeflorus Benth (Leguminosae. The compound characterization processes involve in infrared spectrometry (IR and hydrogen and carbon nuclear magnetic resonance (1H and 13C NMR including experiments in double dimensions (COSY 1H 1H, HMQC and HMBC. Allellopathic effects were evaluated by bioassays, carried out at controlled 25 °C temperature and photoperiod (12h light/12h dark, during 72 hours. Sample concentrations of 1,0, 100,0 and 1000,0 mg.L-1 were tested. Senna occidentalis (fedegoso and Acanthospermum australe (carrapichinho were used as the target weed plants. Was observed that the allelopatic effect of the compounds increased as a function of the enhancement of concentration, thus showing a relation dose dependence. The N-ethyl-6a-acethoxy- 7b-hydroxyvouacapan-17b-amide and N,N-diethyl-6a-acethoxy-7b-hydroxyvouacapan 17b-amide were the derivatives that present the biggest inhibitory effect on seed germination and root growth of fedegoso and carrapichinho. Therefore, these compounds represent the most allelochemical potential against these weeds.

  2. Impact of organic and inorganic fertilizers application on the phytochemical and antioxidant activity of Kacip Fatimah (Labisia pumila Benth).

    Science.gov (United States)

    Ibrahim, Mohd Hafiz; Jaafar, Hawa Z E; Karimi, Ehsan; Ghasemzadeh, Ali

    2013-09-05

    A study was conducted to compare secondary metabolites and antioxidant activity of Labisia pumila Benth (Kacip Fatimah) in response to two sources of fertilizer [i.e., organic (chicken dung; 10% N:10% P₂O₅:10% K₂O) and inorganic fertilizer (NPK green; 15% N, 15% P₂O₅, 15% K₂O)] under different N rates of 0, 90, 180 and 270 kg N/ha. The experiment was arranged in a randomized complete block design replicated three times. At the end of 15 weeks, it was observed that the application of organic fertilizer enhanced the production of total phenolics, flavonoids, ascorbic acid, saponin and gluthathione content in L. pumila, compared to the use of inorganic fertilizer. The nitrate content was also reduced under organic fertilization. The application of nitrogen at 90 kg N/ha improved the production of secondary metabolites in Labisia pumila. Higher rates in excess of 90 kg N/ha reduced the level of secondary metabolites and antioxidant activity of this herb. The DPPH and FRAP activity was also highest at 90 kg N/ha. The results indicated that the use of chicken dung can enhance the production of secondary metabolites and improve antioxidant activity of this herb.

  3. KANDUNGAN KIMIA BERBAGAI EKSTRAK DAUN MIANA (COLEUS BLUMEI BENTH DAN EFEK ANTHELMINTIKNYA TERHADAP CACING PITA PADA AYAM

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    Yusuf Ridwan

    2006-08-01

    Full Text Available Study on the chemical compound of painted nettle (Coleus blumei Benth leave extract and its anthelmintic activity against chicken tapeworm were conducted. Leave of painted nettle were collected and extracted with hexane, chloroform, ethanol and water. Phytochemical analysis was carried out to determine the chemical compound of secondary metabolites. Anthelmintic activity was evaluated with an assay using chicken tapeworm in a serial microplate dilution method by determination of efective concentration 50 (EC50 using probit analysis. The result of phytochemistry analysis showed that Coleus leaves consisted of flavonoid, steroid, tannin and saponin. Three of four extracts displayed strong anthelmintic activity with the higest activity belong to chloroform extract with EC50 5 mg/ml followed by n-hexane 9 mg/ml and metanol extract 10,2 mg/ml, while water extract has a weak anthelmintic activity with 106,2 mg/ml. In general, chloroform extract proved to be a more efficient extractant of biologically active compounds than either hexane, ethanol or water extract. The promising activity displayed by a number of extracts has led to further investigation of the active compound. Unfortunately, the result of invivo assay showed that the chloroform extract treatment with dose level 25 mg/kg BW could not to reduce the number of tapeworm in chicken. It is interesting for further investigate the differences of respon between in vitro and in vivo to determine involved factors.

  4. Antidiabetic activity of methanolic bark extract ofAlbizia odoratissima Benth. in alloxan induced diabetic albino mice

    Institute of Scientific and Technical Information of China (English)

    Dinesh Kumar; Sunil Kumar; Sonia Kohli; Renu Arya; Jyoti Gupta

    2011-01-01

    Objective:To evaluate the antidiabetic potential of methanolic extract ofAlbizia odoratissima Benth. bark in alloxan induced diabetic mice.Methods: Group-Ⅰ (normal control) mice received only basal diet without any treatment. In Group-Ⅱ (Diabetic control) mice, diabetes was induced by alloxan (150 mg/kgi.p.) and received only Tween80, 5% v/v in normal saline. Group-Ⅲ and Group-Ⅳ mice received metformin (10 mg/kg) and gliclazide (10 mg/kg) as standard drugs. Group-Ⅴ and Ⅵ mice received methanolic bark extract ofAlbizia odoratissima at doses of250 and500 mg/kg body weightp.o., respectively.Results: The results of the study indicates thatAlbizia odoratissima bark extract significantly (P<0.01) reduced the blood sugar level. The bark extract also significantly reduced the levels of serum cholesterol, triglycerides, serum glutamic-oxaloacetic transaminase, serum glutamic-pyruvic transaminase, alkaline phosphatase and decreases level of total proteins in alloxan induced diabetic mice.Conclusions:Methanolic extract ofAlbizia odoratissima has protective effects on the protection of vital tissues (pancreas, kidney, liver, heart and spleen), thereby reducing the causation of diabetes in experimental animals.

  5. Activités insecticides de Striga hermonthica (Del. Benth (Scrophulariaceae sur Callosobrichus maculatus (Fab. (Coleoptera : Bruchidae

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    Nacoulma OG.

    2006-01-01

    Full Text Available Insecticidal activities of Striga hermonthica (Del. Benth (Scrophulariacecae on Callobruchus maculatus (Fab. (Coleptera Bruchidae. This paper deals with insecticidal potentialities of Striga hermonthica (Del. (Scrophulariaceae in protection of cowpea Vigna unguculata (L. Walp against Callosobruchus maculatus (Fab. (Coleoptera: Bruchidae during storage. Crude acetone extract at 0,5% w/w (100 mg of extract for 20 g of grain exhibits 48% of ovicidal effect and then reduces by half emergence rate of adult beetles at the first generation. This extract shows a weak insecticide activity against adults of C. maculatus. Petroleum ether fraction (0,4% w/w of the crude extract reveals ovicidal (51% and larvicidal (72% effects which reduce the emergence rate of adults to only 9%. LD50 and LD90 are monitored during crude extract fractionation to follow ovicidal and larvicidal compounds and to evaluate their efficacy during the isolation procedure. One fraction, mainly composed of two triterpenoid compounds has been identified as responsible of the ovicidal activity of S. hermonthica while the origin of the larvicidal activity hasn’t been identified.

  6. EFFECT OF WATER STRESS SIMULATED WITH NaCl, MANNITOL AND PEG (6000 ON THE GERMINATION Erythrina falcata Benth SEEDS

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    Luciana Luiza Pelegrini

    2013-06-01

    Full Text Available http://dx.doi.org/10.5902/198050989295Erythrina falcata Benth. (Fabaceae species is used in agroforestry, restoration of riparian vegetation in flooded areas, and in the recovery of degraded ecosystems. This work aimed to evaluate the possible effects of water stresses on germination of Erythrina falcata seeds. The study was carried out in The Forestry Seeds Laboratory, Universidade Federal do Paraná (UFPR, in Parana state, Brazil. The experimental units consisted in boxes gerbox (25 seeds on 2 filter papers with 10 ml of osmotic solution. Gerbox was sealed with PVC plastic film and led to germinate in Mangelsdorf type chamber (under light continues at 25±2ºC. Six osmotic potentials (0.0; -0.2; -0.4; -0.6; -0.8; and -1.0 MPa were induced with NaCl, manitol and polyethylene glycol (PEG 6000.The lowest tolerance limit to water stress was observed in PEG solutions. The germination limit was -0.2 to -0.4 MPa of PEG, whereas the osmotic potentials tested with NaCl and mannitol did not affect the germination process.

  7. Crescimento e sobrevivência de Anadenanthera macrocarpa (Benth. Brenan (Leguminosae, em uma área de caatinga, Alagoinha, PE

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    Silva Lucia Maria Bezerra da

    2000-01-01

    Full Text Available O estudo do crescimento e da sobrevivência de Anadenanthera macrocarpa (Benth. Brenan foi realizado na Fazenda Geraldão (8°29'28" S e 36°47'00" W, Alagoinha, PE, no período de julho/1996 a junho/1997. selecionaram-se 10 indivíduos adultos de A. macrocarpa dividindo-se suas copas em quadrantes, e quantificaram-se todos os indivíduos jovens acompanhando crescimento e sobrevivência da estação chuvosa de 1996 até a estação chuvosa de 1997 . A estrutura e distribuição espacial de uma população foi estudada em 10 parcelas de 10m x 10m (1.000m² , subdividida em 250 parcelas de 2m x 2m. Os indivíduos estudados no período de um ano totalizaram 344, sendo 211 jovens e 133 juvenis. A estrutura da população consistiu de 192 indivíduos: 170 jovens (88,5%, oito juvenis (4,2% e 14 adultos (7,3%. A distribuição espacial foi do tipo agregado. A. macrocarpa apresentou-se resistente durante a estação seca, devido ao seu alto índice de sobrevivência (73,3% na área estudada.

  8. Repeated-Doses Toxicity Study of the Essential Oil of Hyptis martiusii Benth. (Lamiaceae in Swiss Mice

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    Germana Freire Rocha Caldas

    2013-01-01

    Full Text Available Hyptis martiusii Benth. (Lamiaceae is found in abundance in Northeastern Brazil where it is used in traditional medicine to treat gastric disorders. Since there are no studies reporting the toxicity and safety profile of this species, we investigated repeated-doses toxicity of the essential oil of Hyptis martiusii (EOHM. Swiss mice of both sexes were orally treated with EOHM (100 and 500 mg/kg for 30 days, and biochemical, hematological, and morphological parameters were determined. No toxicity signs or deaths were recorded during the treatment with EOHM. The body weight gain was not affected, but there was an occasional variation in water and food consumption among mice of both sexes treated with both doses. The hematological and biochemical profiles did not show significant differences except for a decrease in the MCV and an increase in albumin, but these variations are within the limits described for the species. The microscopic analysis showed changes in liver, kidneys, lungs, and spleen; however, these changes do not have clinical relevance since they varied among the groups, including the control group. The results indicate that the treatment of repeated-doses with the essential oil of Hyptis martiusii showed low toxicity in mice.

  9. INDUCTION OF HEPATIC GLUTATHIONE-S-TRANSFERASE ACTIVITY BY Orthosiphon stamineus, BENTH IN STZ-INDUCED DIABETIC RATS

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    J.H. CHIN

    2008-01-01

    Full Text Available The aim of this study was to investigate the acute (one-day treatment effect of a methanol extract of Orthosiphon stamineus, Benth on glutathione-S-transferase (GST activity in streptozotocin (STZ-induced diabetic young male and female Sprague Dawley (SD rats. The methanol extract of O. stamineus was administered orally (5, 31.25, 125 and 500 mg/kg to diabetic rats, and the effect on GST activity was measured by the method of Habig et al. (1974. No lethality and no significant changes in body weight and water intake were observed in the treated group as compared to the control group. A significant increase in the activity of GST was observed in the liver S-9 cytosolic fraction of diabetic male SD rats treated with 125 mg/kg (P < 0.01 and 500 mg/kg (P < 0.01 of the methanol extract O. stamineus. Administration of 500 mg/kg (P < 0.01 of the methanol extract of O. stamineus to diabetic female SD rats increased GST activity when compared to the control group. This study indicates that the methanol extract of O. stamineus could affect the activity of GST in rat liver and the effect seen was dose-dependent

  10. Effect of different liming levels on the biomass production and essential oil extraction yield of Cunila galioides Benth.

    Science.gov (United States)

    Mossi, A J; Pauletti, G F; Rota, L; Echeverrigaray, S; Barros, I B I; Oliveira, J V; Paroul, N; Cansian, R L

    2012-11-01

    Poejo is an aromatic and medicinal plant native to highland areas of south Brazil, in acid soils with high Al3+ concentration. The main objective of the present work was to evaluate the effect of liming on the extraction yield of essential oil of three chemotypes of poejo (Cunila galioides Benth). For this purpose, the experiments were performed in a greenhouse, using 8-litre pots. The treatments were four dosages of limestone (0, 3.15, 12.5, and 25 g.L(-1)) and a completely random experimental design was used, with four replications and three chemotypes, set up in a 3 × 4 factorial arrangement. The parameters evaluated were dry weight of aerial parts, essential oil content and chemical composition of essential oil. Results showed that liming affects the biomass production, essential oil yield and chemical composition, with cross interaction verified between chemotype and limestone dosage. For the higher dosage lower biomass production, lower yield of essential oil as well as the lowest content of citral (citral chemotype) and limonene (menthene chemotype) was observed. In the ocimene chemotype, no liming influence was observed on the essential oil yield and on the content of major compounds. The dosage of 3.15 g.L(-1) can be considered the best limestone dosage for the production of poejo for the experimental conditions evaluated.

  11. Evolution of Volatile Flavour Compounds during Fermentation of African Oil Bean (Pentaclethra macrophylla Benth) Seeds for "Ugba" Production.

    Science.gov (United States)

    Nwokeleme, C O; Ugwuanyi, J Obeta

    2015-01-01

    Fermented African oil bean (Pentaclethra macrophylla Benth) seed is a successful and well studied seasoning and snack in parts of Western Africa. GC-MS analysis of fermenting seeds revealed a mixture of several volatile aroma compounds which changed with time and starter organism. During natural mixed culture process 36 volatile compounds including 12 hydrocarbons, 10 esters, 5 alcohols, 2 phenols, 2 ketones, and one each of furan, amine, acid, thiophene, and lactone were identified. When Bacillus subtilis was used in pure culture, 30 compounds comprising 10 hydrocarbons, 8 esters, 3 alcohols, 2 amines, 2 sulfur compounds, and one of each of acid, aldehyde, phenol, ketone, and furan were identified. Sample fermented with B. megaterium produced 29 aroma compounds comprising 9 hydrocarbons, 10 esters, 2 nitrogenous compounds, 2 ketones, 3 alcohols, and one of each of lactone, aldehyde, furan, and amine. Methyl esters of various long chain fatty acids may be key aroma compounds, based on consistency and persistence. Qualitative or quantitative contribution of individual compounds may only be determined following flavour threshold analysis.

  12. Evaluation of in vivo and in vitro anti-inflammatory activity of Ajuga bracteosa Wall ex Benth

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    Raghunath Singh

    2012-05-01

    Full Text Available Ethnopharmacological relevance: Ajuga bracteosa Wall Ex Benth. (Labiateae is described in Ayurveda for the treatment of rheumatism, gout, palsy and amenorrhea.Objective: Present study was aimed to investigate the in vivo and in vitro anti-inflammatory activity of Neelkanthi (whole plant and to support its traditional use.Methods: Methanolic extract of plant Ajuga bracteosa (ABE was investigated for its anti-inflammatory activity in carrageenan induced rat paw oedema, egg albumin induced inflammation in rats and the study was further supported with in vitro antiinflammatory study by using Human red blood cell membrane stabilization (HRBC method. Three doses of the extract (ABE- 250, 500 and 750 mg/kg, i.p. were used in the study and diclofenac sodium (5mg/kg, i.p. was used as standard. Results: ABE (500 and 750 mg/kg, i.p. significantly (P<0.05 reduced increased in paw volume induced by carrageenan and egg albumin. ABE also showed significant stabilization toward HRBC membrane. Conclusions: ABE at the dose of 500 and 750 mg/kg showed potent action on comparison with the standard drug diclofenac sodium.

  13. Cytomictic Anomalous Male Meiosis and 2n Pollen Grain Formation in Mertensia echioides Benth. (Boraginaceae from Kashmir Himalaya

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    Reyaz Ahmad Malik

    2014-01-01

    Full Text Available Presently Mertensia echioides Benth. (Boraginaceae collected from Kashmir Himalaya, India, is cytologically analyzed for the first time revealing 2n=2x=24 (diploid. Interestingly we found 4.3–6.2% syncytic meiocytes/PMCs with 2n=4x=48 (tetraploid in addition to normal meiocytes (2n=24 during male meiosis. These comparatively larger PMCs (pollen mother cells lead to the formation of fertile giant 2n pollen grains. A frequency of 6.4–13.3% PMCs shows transfer of chromatin material at prophase-I and, therefore, results in aneuploid meiocytes. Whole chromatin transfer by the process of cytomixis could also have led to the formation of tetraploid cells. Translocation heterozygosity is also evident in the form of multivalents in 12–17% diploid (2x meiocytes at diakinesis and metaphase-I and is reported for the first time in this species. The syncytes formed depict open chain hexavalent and quadrivalent formation in the three populations with different frequencies. Moreover chromatin stickiness at metaphase-I is observed in 45% of PMCs in population-1 (P-1. Syncyte or unreduced PMC formation leading to unreduced fertile gametes is here speculated to act as a possible way out for infraspecific polyploidization in the species.

  14. HPLC and GC-MS Determination of Bioactive Compounds in Microwave Obtained Extracts of Three Varieties of Labisia pumila Benth.

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    Hawa Z.E. Jaafar

    2011-08-01

    Full Text Available Microwave extraction of phytochemicals from medicinal plant materials has generated tremendous research interest and shown great potential. This research highlights the importance of microwave extraction in the analysis of flavonoids, isoflavonoid and phenolics and the antioxidant properties of extracts from three varieties of the Malaysian medicinal herb, Labisia pumila Benth. High and fast extraction performance ability, equal or higher extraction efficiencies than other methods, and the need for small samples and reagent volumes are some of the attractive features of this new promising microwave assisted extraction (MAE technique. The aims of the present research were to determine the foliar phenolics and flavonoids contents of extracts of three varieties of L. pumila obtained by a microwave extraction method while flavonoid, isoflavonoid and phenolic compounds were analyzed using RP-HPLC. Furthermore, the antioxidant activities were measured by the DPPH and FRAP methods and finally, the chemical composition of the crude methanolic extracts of the leaves of all three varieties were analyzed by GS-MS.

  15. Antifertility effects of methanolic pod extract of Albizzia lebbeck (L.) Benth in male rats

    Institute of Scientific and Technical Information of China (English)

    R.S.Gupta; J.B.S.Kachhawa; R.Chaudhary

    2004-01-01

    Aim: To evaluate the antifertility activity of the methanolic pod extract of Albizzia lebbeck (L.) Benth in male albino rats. Methods: The methanolic pod extract of Albizzia lebbeck was administrated orally for 60 days at 50, 100 and 200 mg·kg-1·day-1 to male albino rats. Sperm motility and density in cauda epididymides were assessed.Biochemical and histological analysis were performed in blood samples and reproductive organs. Results: A. lebbeck pod extract brought about a significant decrease in the weights of testis, seminal vesicles, epdidymis and ventral prostate. The sperm motility and density were significantly reduced. There was a marked reduction in the numbers of primary spermatocytes, secondary spermatocytes and spermatids. The Sertoli cell count as well as its cross sectional surface area were significantly decreased. The Leydig cell nuclear area and the number of mature Leydig cells were also significantly decreased. The protein, glycogen and cholesterol content of the testis, the fructose in the seminal vesicles and protein in the epididymis were significantly decreased. The RBC and WBC counts, haemoglobin,haematocrit and blood sugar were within the normal range. Conclusion: The methanolic extract of A. lebbeck pods causes spermatogenic arrest in male albino rats.

  16. Antinoceptive and Anti-inflammatory Activities of the Ethanolic Extract, Fractions and Flavones Isolated from Mimosa tenuiflora (Willd. Poir (Leguminosae.

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    Mariluze P Cruz

    Full Text Available The bark of Mimosa tenuiflora (Willd. Poiret (Leguminosae family, popularly known as "jurema preta" in Brazil, is used by the population of Contendas of Sincorá (Bahia State, Brazil for the treatment of coughs and wound healing. Thus, the aim of this study was to evaluate the antinociceptive and anti-inflammatory activities of the bark ethanol extract (EEMT and solvent soluble fractions (hexane-H, DCM-D, EtOAc-E and BuOH-B of the extract in vivo. Additionally, we synthesized 5,7-dihidroxy-4'-methoxyflavanone (isosakuranetin and isolated the compound sakuranetin, and both compounds were also tested. The anti-inflammatory and antinociceptive assays performed were: writhing test; nociception induced by intraplantar formalin injection; leukocyte recruitment to the peritoneal cavity; evaluation of vascular permeability (Evans blue test; and evaluation of mechanical hypernociception (von Frey test. Production of TNF-α, IL-10, myeloperoxidase and the expression of ICAM-1 were also evaluated. Statistical analysis was performed by one-way ANOVA followed by the Bonferroni post-test (n = 8, with P < 0.05. The EEMT showed antinociceptive activities in writhing test (100-200 mg/kg, in the second phase of the formalin test (50-200 mg/kg, and in mechanical hypernociception (100 mg/kg. EEMT showed an anti-inflammatory effect by reducing neutrophil migration to the peritoneal cavity and in the plantar tissue detected by the reduction of myeloperoxidase activity (100 mg/kg, reduction of IL-10 levels and expression of ICAM-1 in the peritoneal exudate and the mesentery (100 mg/kg, respectively. The four soluble EEMT fractions showed good results in tests for antinociceptive (H, D, E, B and anti-inflammation (H, D, E. Only sakuranetin showed reduction of the writhing and neutrophil migration (200 mg/kg. Thus, the EEMT and soluble fractions of M. tenuiflora bark demonstrated great antinociceptive and anti-inflammatory activities, as also sakuranetin. More studies

  17. Antinoceptive and Anti-inflammatory Activities of the Ethanolic Extract, Fractions and Flavones Isolated from Mimosa tenuiflora (Willd.) Poir (Leguminosae).

    Science.gov (United States)

    Cruz, Mariluze P; Andrade, Cassya M F; Silva, Kelle O; de Souza, Erika P; Yatsuda, Regiane; Marques, Lucas M; David, Juceni P; David, Jorge M; Napimoga, Marcelo H; Clemente-Napimoga, Juliana T

    2016-01-01

    The bark of Mimosa tenuiflora (Willd.) Poiret (Leguminosae family), popularly known as "jurema preta" in Brazil, is used by the population of Contendas of Sincorá (Bahia State, Brazil) for the treatment of coughs and wound healing. Thus, the aim of this study was to evaluate the antinociceptive and anti-inflammatory activities of the bark ethanol extract (EEMT) and solvent soluble fractions (hexane-H, DCM-D, EtOAc-E and BuOH-B) of the extract in vivo. Additionally, we synthesized 5,7-dihidroxy-4'-methoxyflavanone (isosakuranetin) and isolated the compound sakuranetin, and both compounds were also tested. The anti-inflammatory and antinociceptive assays performed were: writhing test; nociception induced by intraplantar formalin injection; leukocyte recruitment to the peritoneal cavity; evaluation of vascular permeability (Evans blue test); and evaluation of mechanical hypernociception (von Frey test). Production of TNF-α, IL-10, myeloperoxidase and the expression of ICAM-1 were also evaluated. Statistical analysis was performed by one-way ANOVA followed by the Bonferroni post-test (n = 8), with P < 0.05. The EEMT showed antinociceptive activities in writhing test (100-200 mg/kg), in the second phase of the formalin test (50-200 mg/kg), and in mechanical hypernociception (100 mg/kg). EEMT showed an anti-inflammatory effect by reducing neutrophil migration to the peritoneal cavity and in the plantar tissue detected by the reduction of myeloperoxidase activity (100 mg/kg), reduction of IL-10 levels and expression of ICAM-1 in the peritoneal exudate and the mesentery (100 mg/kg), respectively. The four soluble EEMT fractions showed good results in tests for antinociceptive (H, D, E, B) and anti-inflammation (H, D, E). Only sakuranetin showed reduction of the writhing and neutrophil migration (200 mg/kg). Thus, the EEMT and soluble fractions of M. tenuiflora bark demonstrated great antinociceptive and anti-inflammatory activities, as also sakuranetin. More studies

  18. 广藿香抗青枯病鉴定方法的研究%Study on Bacterial-wilt-resistance Identification Methods for Pogostemon cablin (Blanco) Benth.

    Institute of Scientific and Technical Information of China (English)

    贺红; 温雁鹰; 许仕仰; 梁志毅

    2011-01-01

    Objective To investigate the optimal concentration and inoculation procedure of crude toxin from Ralstonia solanacearum, and to establish the identification method for bacterial wilt resistance of Pogostemon cablin (Blanco) Benth. seedling, so as to lay the foundation for resistance breeding of Pogostemon cablin (Blanco)Benth.. Methods The growth curve of Ralstonia solanacearum was determined. And then we studied the effect of seedling age, crude toxin concentrations and inoculation ways for the crude toxin on the pathogenicity of Pogostemon cablin (Blanco) Benth.. Results The growth of Ralstonia solanacearum presented as the S-shaped curve, the highest concentration being 12. 46 × l08 cfu/mL. The seedling of Pogostemon cablin (Blanco) Benth. aged 100 days was optimal for the identification of bacterial wilt resistance. In the crude toxin concentration range of 0. 4 ×108 ~ 0. 6 × 108 cfu/mL, the inoculated plant showed a moderately advanced progress of bacterial wilt, which was suitable for the resistance identification. Having the advantage of shortening the experiment period, the rootsubmerging method was recommended for the resistance identification of Pogostemon cablin (Blanco) Benth..Conclusion The indoor identification method for Pogostemon cablin (Blanco) Benth. resistance to bacterial wilt has been established preliminarily.%[目的]对广藿香幼苗接种青枯茵粗毒素,观察其感病状况,建立广藿香苗期抗性鉴定方法,为广藿香抗病育种奠定基础.[方法]测定不同培养时间的青枯茵菌液浓度,绘制青枯菌的生长曲线;分别设置不同广藿香苗龄、不同浓度青枯菌制备粗毒素及不同接种方法等试验,探讨影响致病性的因素.[结果]青枯茵的生长曲线呈"S"型,青枯茵菌液浓度在稳定期最高可达12.46×108cfu/mL.对于广藿香抗青枯病的苗期鉴定,以100d左右苗龄的植株较适宜;在0.4×108~0.6×108cfu/mL浓度范围内,接种植株表现渐进的发病过

  19. PHYTOCHEMICAL ANALYSIS OF THREE ENDANGERED PLANTS (COSTUS SPECIOUS, GLORIOSSA SUPERBA LINN AND RAUVOLFIA SERPENTINE (LINN BENTH FROM KANKER DISTRICT OF CHHATTISGARH, INDIA

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    NITISH KUMAR SAHU

    2013-01-01

    Full Text Available Chhattisgarh, the herbal state houses with rich and unique biodiversity of medicinal plants which are widely usedby traditional healers of the state for treatment of various disease and extensively exploited for commercialpurpose which leads to serious threat. Several plants have been studied and listed as rare and endangered whichneed to be conserved. We selected three such plants viz. Costus specious, Gloriossa superba Linn and Rauvolfiaserpentine (Linn Benth., from Kanker district of Chhattisgarh for their phytochemical analysis by chemicalscreening and Thin layer chromatography. On primary analysis of the methanolic and ethanolic extract ofrhizome of C. specious and G. superba and root and leaves of R. serpentine (Linn Benth. we found the presenceof phytocompounds like saponin, tannins, alkaloids, terpenoids, flavonoids and glycosides in them. Thisphytocompounds were further separated by TLC using petroleum ether: methanol: benzene (8:1:1.5 as mobilephase in silica gel coated glass plates. Maximum 9 bands were observed in methanolic extract of rhizome of C.specious with Rf value between 0.05 – 0.97 and 6 bands in methanolic extract of rhizome of G. superba with Rfvalue between 0.02 – 0.94.Similarily in ethanolic and methanolic extract of leaves of R. serpentine (Linn Benth.Maximum 13 bands were observed with Rf value between 0.05 – 0.96 and 0.04 – 0.98 respectively whereasethanolic extract of root showed maximum 11 bands with Rf value between 0.04 – 0.98 in comparison withchloroform and petroleum ether extracts. This result can further help in development of new drugs for diseaseslike cancer and hypertension based on their traditional uses.

  20. 分光光度法测定角花胡颓子中的总黄酮%Determination of total flavonoid in Elaeagnus gonyanthes Benth by spectrophotometry

    Institute of Scientific and Technical Information of China (English)

    蒋德锡; 陈文芝; 贾建静; 张丹

    2011-01-01

    OBJECTIVE To establish a spectrophotometric method for determination of total flavonoids in Elaeagnus gonyanthes Benth.METHODS Selecting rutin as control article, colorating with AlCl3, a spectrophotometric method was adopted to determine the contents of flavonoids.The total flavonoids in Elaeagnus gonyanthes Benth were calculated.RESULTS The linear concentration range of total flavonoids was 4.4 - 35.2 μg· mL- 1 ( r = 0.9999) and the average recovery of total flavoniods was 96.21% with RSD of 1.39% (n =9).CONCLUSION This method is simple,quick,accurate and can be used for quality control of Elaeagnus gonyanthes Benth.%目的:采用分光光度法测定角花胡颓子中总黄酮的含量.方法:以芦丁为对照品,三氯化铝显色,在417nm测定吸光度,计算角花胡颓子中总黄酮的含量.结果:芦丁4.4-35.2μg·mL-1与吸光度值呈良好的线性关系,回归方程为:Y=0.043X+0.0008(r=0.9999),平均加样回收率为96.21%,RSD=1.39%(n=9).结论:所建方法简便、快速、准确,可用于角花胡颓子的质量控制.

  1. 5-H¡droxi-2-Metil-1,4-Naftoquinona obtenida de Pera nítida (Benth. Jablonski(Euforbiaceae

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    Luis E. Cuca S.

    2009-07-01

    Full Text Available Del extracto clorofórmico de la corteza del tronco de Pera nítida (Benth. Jablonski se aisló una sustancia amarilla que fue identificada como 5—hidro.x¡-2— metil —1,4—naftoquinona. Esta sustancia es conocida como plumbagina y ha mostrado una destacada acción biológica. La estructura fue deducida con base en los datos espectroscópicos y su presencia en esta especie tiene importancia quimiotaxonómica.

  2. Essential oil composition of the aerial parts of fresh and air-dried Salvia palaestina Benth. (Lamiaceae) growing wild in Jordan.

    Science.gov (United States)

    Al-Jaber, Hala I; Al-Qudah, Mahmoud A; Barhoumi, Lina M; Abaza, Ismail F; Afifi, Fatma U

    2012-01-01

    The composition of the essential oil of fresh and air-dried Salvia palaestina Benth. (Lamiaceae) growing wild in Jordan has been studied using gas chromatography-mass spectrometry analysis. The essential oils of fresh and air-dried S. palaestina were mainly composed of sesquiterpene hydrocarbons (52.66% and 65.98%, respectively). The major component detected in the oils of fresh and dry S. palaestina was germacrene D (21.18% and 26.02%, respectively). Air drying resulted in a general increase of sesquiterpene hydrocarbons and a great decrease in the percentage of monoterpene hydrocarbons.

  3. Simultaneous Quantification of Limonin, Two Indolequinazoline Alkaloids, and Four Quinolone Alkaloids in Evodia rutaecarpa (Juss.) Benth by HPLC-DAD Method

    OpenAIRE

    Pei-ting Zhang; Bi-yan Pan; Qiong-feng Liao; Mei-cun Yao; Xin-jun Xu; Jin-zhi Wan; Dan Liu; Zhi-yong Xie

    2013-01-01

    A simple and efficient HPLC-DAD (225 nm) method was developed and validated for the simultaneous determination of limonin and six key alkaloids (evodiamine, rutaecarpine, 1-methyl-2-undecyl-4(1H)-quinolone, evocarpine, 1-methy-2-[(6Z,9Z)]-6,9-pentadecadienyl-4-(1H)-quinolone, and dihydroevocarpine) in Evodia rutaecarpa (Juss.) Benth, which has been widely used as one of the Traditional Chinese Medicines. The chromatographic separation was carried out on a Hypersil BDS C18 column, and gradient...

  4. Caracterização botânica e química de Rudgea viburnoides (Cham. Benth., (Rubiaceae Morphological and chemical diagnosis of Rudgea viburnoides (Cham. Benth., (Rubiaceae

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    R.M.S. Alves

    2004-01-01

    Full Text Available Congonha, congonha-de-bugre e bugre são nomes populares atribuídos a várias espécies medicinais, incluindo Rudgea viburnoides (Cham. Benth. (Rubiaceae, cujas folhas são utilizadas na medicina popular como diuréticas, hipotensoras, antireumáticas e depurativas do sangue. Esta espécie vem sendo comercializada como porangaba e utilizada em regimes de emagrecimento, substituindo Cordia ecalyculata Vell. (Boraginaceae. Neste estudo, foram determinadas as características botânicas, fisico-químicas e químicas para R. viburnoides com o objetivo de auxiliar sua identificação taxonômica e o controle de qualidade de produtos preparados com esta planta. Uma amostra referência foi coletada e submetida à caracterização macroscópica, microscópica e organoléptica, determinação de cinzas totais e umidade, prospecção fitoquímica e determinação dos perfis cromatográficos em CCD. Amostras de R. viburnoides podem ser reconhecidas pela epiderme pilosa com marcantes estrias cuticulares, estômatos paracíticos, presença de idioblastos com ráfides no mesofilo e pela configuração em arco dos tecidos vasculares da nervura de primeira ordem, caracteres ausentes em C. ecalyculata. A presença de cistólitos e canais mucilaginosos na medula diferem prontamente C. ecalyculata de R. viburnoides. A análise por CCD evidenciou a presença de flavonóides e saponinas. Amostras adquiridas no comércio e submetidas aos mesmos ensaios permitiram validar os métodos propostos e confirmaram a ampla comercialização de R. viburnoides em Minas Gerais.Congonha, congonha-de-bugre and bugre are popular names for several medicinal plant species, including Rudgea viburnoides (Cham. Benth. (Rubiaceae, whose leaves are widely used in popular medicine as diuretic, hypotensive, antirheumatic, and blood depurative. This species has been commercialized as porangaba, and used for diet purposes in substitution of Cordia ecalyculata Vell. (Boraginaceae. This study

  5. Crescimento de mudas de angico-vermelho (Anadenanthera macrocarpa (Benth. Brenan sob diferentes doses de macronutrientes Growth of angico vermelho (Anadenanthera macrocarpa (Benth. Brenan seedlings under different macronutrient doses

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    Elzimar de Oliveira Gonçalves

    2008-12-01

    Full Text Available Neste trabalho, objetivou-se avaliar, por meio de características morfológicas, o efeito de doses de macronutrientes no crescimento e desenvolvimento de mudas de angico- vermelho (Anadenanthera macrocarpa (Benth. Brenan produzidas em amostras de três classes de solo (Argissolo Vermelho-Amarelo, Latossolo Vermelho-Amarelo álico e Latossolo Vermelho-Amarelo distrófico. As mudas foram plantadas em vasos com capacidade para 2,1 dm³. O experimento foi conduzido no período de dezembro de 2004 a maio de 2005. Os tratamentos foram delimitados segundo uma matriz baconiana, em que se variaram os macronutrientes em três doses diferentes, mais dois tratamentos adicionais (zero e base, com quatro repetições. As fontes de N, K e S foram parceladas em quatro vezes (0-30-60-90 dias. Em termos gerais, o maior crescimento das plantas ocorreu no Latossolo Vermelho-Amarelo álico. O nutriente que mais surtiu efeitos significativos foi o P, sendo recomendadas doses de 150 a 250 mg dm-3. Para a aplicação de N, recomendam-se doses mínimas de 50 mg/dm³. A aplicação de S deve ser de 20 a 80 mg dm-3. As respostas à aplicação dos demais nutrientes (Ca, Mg e K, em muitos casos, não ocorreu, evidenciando-se que a espécie tem baixo requerimento por eles. Entretanto, partindo desse estudo, sugerem-se novos estudos com os nutrientes K e Ca, para produção de mudas da referida espécie nesses solos.This work aimed to evaluate, by means of morphological characteristics, the effect of macronutrient doses on the growth of angico vermelho (Anadenanthera macrocarpa (Benth. Brenan seedlings cultivated in three soils (Red Yellow Argisol, Red Yellow Dystrophic Latosol and Red Yellow alic Latosol. The seedling were sown in vases with capacity of 2.1 dm³ . The experiment was carried out from December 2004 to June 2005. The treatments were delimited according to a Baconian matrix, with macronutrients varying in three different doses, plus two additional treatments

  6. Caracterização farmacognóstica das folhas e sementes de Albizia lebbeck (L. Benth. (Fabaceae Pharmacognostic characterization of leaves and seeds of Albizia lebbeck (L. Benth. (Fabaceae

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    Cláudia G. Miranda

    2009-06-01

    Full Text Available Albizia lebbeck (L. Benth. é popularmente conhecida no Brasil por coração-de-negro. É uma espécie aclimatada nesse país. As sementes dessa espécie são utilizadas no tratamento de diarréia, disenteria e hemorróidas e as folhas, no tratamento de úlceras e nas conjuntivites alérgicas. Este trabalho foi realizado com a finalidade de determinar as características macroscópicas e microscópicas das folhas e sementes dessa planta, uma vez que estas são as partes usadas popularmente. Algumas características de valor na sua diagnose foram apontadas e ilustradas por fotomicrografias. Reações de fitoquímica e a determinação do conteúdo de água e de cinzas foram realizadas no pó das folhas e sementes de A. lebbeck. Saponinas, alcalóides e cumarinas foram detectados nas folhas e sementes; flavonóides foram também identificados nas folhas. O teor de umidade foi 6,78% e 7,91%, respectivamente, para o pó das folhas e sementes. Os valores de cinzas totais e cinzas insolúveis em ácido foram 9,60% e 0,62% para as folhas e 4,13% e 0,21% para as sementes. Os dados obtidos são importantes para o controle de qualidade dessa planta medicinal. O extrato bruto da planta não apresentou atividade antibacteriana.Albizia lebbeck (L. Benth., is popularly known in Brazil as "coração-de-negro". It is a species acclimatized in Brazil. The seeds of this species are used to treat diarrhea, dysentery and hemorrhoids and the leaves are used for the treatment of ulcers and allergic conjunctivitis. The purpose of this work was to determine the macroscopic and microscopic characteristics of the leaves and seeds of this plant, since these are the parts used popularly. Some characters of diagnostical value are pointed out and illustrated by photomicrographs. Phytochemistry reactions and determination of water and ash were done on the powder of A. lebbeck. Saponins, alkaloids, coumarins were detected in the leaves and seeds; flavonoids were also detected

  7. Avaliação do efeito antinociceptivo e da toxicidade aguda do extrato aquoso da Hyptis fruticosa Salmz. ex Benth Evaluation of the analgesic effect and acute toxicity of the aqueous extract of Hyptis fruticosa (Salmz. ex Benth.

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    Aline B.L. Silva

    2006-12-01

    Full Text Available Este trabalho descreve o efeito antinociceptivo e a toxicidade aguda do extrato aquoso das folhas da Hyptis fruticosa Salmz. ex Benth. (Lamiaceae. O extrato aquoso liofilizado, administrado por via oral, reduziu as contorções abdominais induzidas por ácido acético (200, 400 e 500 mg/kg e o tempo de reação dos animais na primeira fase do teste da formalina (100 mg/kg e 400 mg/kg. No teste da placa quente, o extrato aquoso aumentou o tempo de latência ao calor (100 e 200 mg/kg tendo este efeito sido revertido pelo antagonista opióide naloxona (5 mg/kg; i.p.. No ensaio de toxicidade aguda, não foi detectada a morte de nenhum animal após tratamento com doses de até 5 g/kg (v.o. do extrato. Em conclusão, os resultados obtidos indicam que o extrato aquoso da Hyptis fruticosa apresenta efeito antinociceptivo em camundongos e não apresenta toxicidade aguda nas doses testadas.The antinociceptive effect and the acute toxicity of Hyptis fruticosa leaves were evaluated through the administration of its aqueous extract in mice. The extract, administered orally (200, 400, and 500 mg/kg, reduced the nociceptive response in the writhing test as well as in the early phase of the formalin test (100 and 400 mg/kg and it increased the latency time in the hot plate test (100 and 200 mg/kg. The antinociceptive effect was reversed by naloxone (5 mg/kg, i.p.. Moreover, no animal deaths were observed in doses up to 5 g/kg. In conclusion, the aqueous extract of Hyptis fruticosa showed no acute toxicity at the evaluated doses and revealed antinociceptive effect in mice. Such effects are possibly associated with the opioid system activation.

  8. Variation in the accumulation levels of N,N-dimethyltryptamine in micropropagated trees and in in vitro cultures of Mimosa tenuiflora.

    Science.gov (United States)

    Nicasio, María Del Pilar; Villarreal, María Luisa; Gillet, Françoise; Bensaddek, Lamine; Fliniaux, Marc-André

    2005-01-01

    The present article reports the accumulation of N,N-dimethyltryptamine and its metabolic precursors (tryptophan, tryptamine) in different organs of micropropagated Mimosa tenuiflora trees (leaves, flowers and bark) subjected to seasonal variations (January and June), as well as in in vitro cultures (plantlets and calluses) of this plant species. The accumulation of all the tested compounds varied according to the organ, the month of collection, and age of the plant material. In all cases, the neurotoxic compound N,N-dimethyltryptamine (DMT) was detected with the lowest concentration 0.01% dry weight (DW) in flowers, and the highest 0.33% DW in bark. For the in vitro cultures, DMT was present in high yields in plantlets (0.1-0.2% DW), while in calluses this compound was initially detected but its concentration decreased significantly in the subsequent subcultures.

  9. Teratogenic effects of Mimosa tenuiflora in a rat model and possible role of N-methyl- and N,N-dimethyltryptamine.

    Science.gov (United States)

    Gardner, Dale; Riet-Correa, Franklin; Lemos, Danilo; Welch, Kevin; Pfister, James; Panter, Kip

    2014-07-30

    Mimosa tenuiflora is a shrub/tree found in northeastern Brazil sometimes eaten by livestock and believed to be responsible for malformations observed in many animals from that region. The teratogenic compounds in M. tenuiflora are not known. This study used pregnant rats fed M. tenuiflora and components therefrom for bioassay and fractionation of possible teratogenic compounds. Rat pups were examined for cranial-facial defects and skeletal malformations. Experimental diets included M. tenuiflora leaf and seed material, extracts of leaf and seed, alkaloid extracts of leaf and seed, and N-methyltryptamine and N,N-dimethyltryptamine. Pups from mothers who received M. tenuiflora plant material, methanol extracts, alkaloid extracts, and purified N-methyltryptamines had a higher incidence of soft tissue cleft palate and skeletal malformations. Results are summarized as to the frequency of observed cleft palate and other noted malformations for each diet versus control.

  10. Phylogeny based on 16S rDNA and nifH sequences of Ralstonia taiwanensis strains isolated from nitrogen-fixing nodules of Mimosa pudica, in India.

    Science.gov (United States)

    Verma, Subhash Chandra; Chowdhury, Soumitra Paul; Tripathi, Anil Kumar

    2004-05-01

    Bacterial symbionts present in the indeterminate-type nitrogen (N)-fixing nodules of Mimosa pudica grown in North and South India showed maximum similarity to Ralstonia taiwanensis on the basis of carbon-source utilization patterns and 16S rDNA sequence. Isolates from the nodules of M. pudica from North India and South India showed identical ARDRA (Amplified Ribosomal DNA Restriction Analysis) patterns with Sau3AI and RsaI, but AluI revealed dimorphy between the North Indian and South Indian isolates. Alignment of 16S rDNA sequences revealed similarity of North Indian isolates with an R. taiwanensis strain isolated from M. pudica in Taiwan, whereas South Indian isolates showed closer relatedness with the isolates from Mimosa diplotricha. Alignment of nifH sequences from both North Indian and South Indian isolates with that of the related isolates revealed their closer affinity to alpha-rhizobia, suggesting that nif genes in the beta-rhizobia might have been acquired from alpha-rhizobia via lateral transfer during co-occupancy of nodules by alpha-rhizobia and progenitors of R. taiwanensis, members of the beta-subclass of Proteobacteria. Immunological cross-reaction of the bacteroid preparation of M. pudica nodules showed strong a positive signal with anti-dinitrogenase reductase antibody, whereas a weak positive cross-reaction was observed with free-living R. taiwanensis grown microaerobically in minimal medium with and without NH4Cl. In spite of the expression of dinitrogenase reductase under free-living conditions, acetylene reduction was not observed under N-free conditions even after prolonged incubation.

  11. Lowering blood pressure effect of Apium graviolens (seledri and Orthosipleon stamineus benth (kumis kucing in mild and moderate hypertension

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    Siti Supari

    2002-12-01

    Full Text Available In Indonesia Apium graviolens and Orthosiphon stamineus benth have been used to as traditional medicines to lower blood pressure. Since at present time is avaiLable as a combination of those phytopharmaca in the market, therefore, it is necessary to conduct a study to evaLtnte the ffictivity and side effects of those phytopharmaca toward hypertensive subjects. A randomized double blind conftolled trial was conducted at area of Monica - Jakarta in South of Jalarta from July-untiL 29 October 2001 toward mild and moderate hypertensive subjects. The first group (72 subjects received phytophanmaca (Tensigard® /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin-top:0in; mso-para-margin-right:0in; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0in; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} which contains Apium graviolens and Orthosiphon stamineus benth 3 x 250 mg, whiLe the second grottp (70 subjects had Amlodipine I x 5 mg. Obseruation were conducted for 12 weelcs, and the parameter observed wère systolic and diastolic blood pressure (SBP and DBP, plasma lipid, blood glucose before and after treatment, and electrolyte with nvo week intervals. The Resuhs revealed that the phytopharmaca treatment for 12 weel 0,05; DBP 10.00 t0.96 mmHg vs 9.49 t 1.37 ntmHg; P> 0.05. Phytopharmaca treatment for 12

  12. Phytochemical Analysis and Antimicrobial Activities of Methanolic Extracts of Leaf, Stem and Root from Different Varieties of Labisa pumila Benth

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    Ehsan Karimi

    2011-05-01

    Full Text Available A local herb, Kacip Fatimah, is famous amongst Malay women for its uses in parturition; however, its phytochemical contents have not been fully documented. Therefore, a study was performed to evaluate the phenolics, flavonoids, and total saponin contents, and antibacterial and antifungal properties of the leaf, stem and root of three varieties of Labisia pumila Benth. Total saponins were found to be higher in the leaves of all three varieties, compared to the roots and stems. Leaves of var. pumila exhibited significantly higher total saponin content than var. alata and lanceolata, with values of 56.4, 43.6 and 42.3 mg diosgenin equivalent/g dry weight, respectively. HPLC analyses of phenolics and flavonoids in all three varieties revealed the presence of gallic acid, caffeic acid, rutin, and myricetin in all plant parts. Higher levels of flavonoids (rutin, quercitin, kaempferol were observed in var. pumila compared with alata and lanceolata, whereas higher accumulation of phenolics (gallic acid, pyrogallol was recorded in var. alata, followed by pumila and lanceolata. Antibacterial activities of leaf, stem and root extracts of all varieties determined against both Gram positive (Micrococcus luteus, Bacillus subtilis B145, Bacillus cereus B43, Staphylococcus aureus S1431 and Gram negative (Enterobacter aerogenes, Klebsiella pneumonia K36, Escherichia coli E256, Pseudomonas aeruginosa PI96 pathogens showed that crude methanolic extracts are active against these bacteria at low concentrations, albeit with lower antibacterial activity compared to kanamycin used as the control. Antifungal activity of methanolic extracts of all plant parts against Fusarium sp., Candida sp. and Mucor using the agar diffusion disc exhibited moderate to appreciable antifungal activities compared to streptomycin used as positive control.

  13. Phytochemical analysis and antimicrobial activities of methanolic extracts of leaf, stem and root from different varieties of Labisa pumila Benth.

    Science.gov (United States)

    Karimi, Ehsan; Jaafar, Hawa Z E; Ahmad, Sahida

    2011-05-27

    A local herb, Kacip Fatimah, is famous amongst Malay women for its uses in parturition; however, its phytochemical contents have not been fully documented. Therefore, a study was performed to evaluate the phenolics, flavonoids, and total saponin contents, and antibacterial and antifungal properties of the leaf, stem and root of three varieties of Labisia pumila Benth. Total saponins were found to be higher in the leaves of all three varieties, compared to the roots and stems. Leaves of var. pumila exhibited significantly higher total saponin content than var. alata and lanceolata, with values of 56.4, 43.6 and 42.3 mg diosgenin equivalent/g dry weight, respectively. HPLC analyses of phenolics and flavonoids in all three varieties revealed the presence of gallic acid, caffeic acid, rutin, and myricetin in all plant parts. Higher levels of flavonoids (rutin, quercitin, kaempferol) were observed in var. pumila compared with alata and lanceolata, whereas higher accumulation of phenolics (gallic acid, pyrogallol) was recorded in var. alata, followed by pumila and lanceolata. Antibacterial activities of leaf, stem and root extracts of all varieties determined against both Gram positive (Micrococcus luteus, Bacillus subtilis B145, Bacillus cereus B43, Staphylococcus aureus S1431) and Gram negative (Enterobacter aerogenes, Klebsiella pneumonia K36, Escherichia coli E256, Pseudomonas aeruginosa PI96) pathogens showed that crude methanolic extracts are active against these bacteria at low concentrations, albeit with lower antibacterial activity compared to kanamycin used as the control. Antifungal activity of methanolic extracts of all plant parts against Fusarium sp., Candida sp. and Mucor using the agar diffusion disc exhibited moderate to appreciable antifungal activities compared to streptomycin used as positive control.

  14. Árvores de Baginha (Stryphnodendron guianense (Aubl. Benth. em Ecossistemas de Pastagens Cultivadas na Amazônia Ocidental

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    Andrade Carlos Mauricio Soares de

    2002-01-01

    Full Text Available Foi estudado o efeito de árvores isoladas de baginha (Stryphnodendron guianense (Aubl. Benth. sobre alguns aspectos do ecossistema de pastagens cultivadas na Amazônia Ocidental. Avaliaram-se, sob a copa das árvores e na área adjacente a pleno sol, a fertilidade do solo, a serapilheira, o teor de água no solo, a transmissão de luz ao pasto, a disponibilidade de forragem e as composições química e morfológica das gramíneas (mistura de Brachiaria decumbens e B. brizantha cv. Marandu. As árvores de baginha tiveram efeito positivo sobre a fertilidade do solo, notadamente com relação ao conteúdo de matéria orgânica e de nitrogênio, sendo a deposição de grande quantidade de serapilheira, rica em nitrogênio, a principal contribuição. A forragem sombreada apresentou maiores teores de N e K e menores de Ca, na fração folha verde, do que em áreas a pleno sol. As árvores de baginha apresentaram copa pouco densa, permitindo a transmissão de 27% da radiação fotossinteticamente ativa incidente ao meio-dia. A elevada cobertura do solo (superior a 85% e a boa disponibilidade de forragem sob a copa da baginha, juntamente com outros atributos, confirmam o potencial desta leguminosa para arborização de pastagens e como componente de outras modalidades de sistemas silvipastoris no trópico úmido.

  15. Estudo do sistema de reprodução da fava-d'anta (Dimorphandra mollis Benth.

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    A.D.R. Mendes

    2013-01-01

    Full Text Available Entre as plantas nativas de uso medicinal do Cerrado brasileiro encontra-se a fava-d'anta (Dimorphandra mollis Benth com alto potencial econômico por possuir inúmeras potencialidades medicinais e fitoquímicas. A indústria extrai dos frutos os princípios ativos rutina, quercetina, e ramnose, dentre outros, usados na fabricação de medicamentos e cosméticos, principalmente no exterior. O conhecimento do sistema reprodutivo da espécie é fundamental para sua conservação e manejo. O presente trabalho objetivou determinar as características morfométricas das flores e caracterizar o sistema reprodutivo da D. mollis em área de Cerrado inalterada antropomorficamente, no município de Olhos D'água - MG. Para as características morfométricas, diâmetro da flor, comprimento da flor, do ovário, e da antera, foram utilizadas cinco flores em pré-antese. Para a determinação do sistema reprodutivo utilizou-se a razão pólen:óvulo (P:O, em 40 flores. As flores da D. mollis apresentaram comprimento da flor de 3,00 mm, diâmetro da flor de 2,00 mm, comprimento do óvulo de 2,60 mm, comprimento da antera de 1,57 mm, e o número de óvulos e de anteras foram 20 e 5,8, respectivamente. A razão P:O foi 765,030, sendo que esse índice não é afetado pelas características morfométricas. O sistema reprodutivo da D. mollis foi classificado como alógama facultativa.

  16. Insectos plagas y benéficos asociados al cultivo de mora (Rubus glaucus, benth en La Sabana, Madriz, Nicaragua

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    Edgardo Jiménez-Martínez

    2006-12-01

    Full Text Available LA MORA (RUBUS GLAUCUS, BENTH ES UNA PLANTA en proceso de domesticación que se cultiva en pequeñas parcelas. Es hospedera de muchos insectos plaga y enfermedades. En Nicaragua se establecieron plantaciones de mora desde hace unos tres años, como alternativa de diversificación de fincas cafetaleras en los departamentos de Madriz y Nueva Segovia. Hasta ahora no hay información formal sobre los principales insectos plaga y benéficos presentes en este cultivo, que está tomando gran importancia. Por ello, se hizo una investigación para describir la fluctuación poblacional de los insectos plaga y sus depredadores naturales, asociados a este cultivo. El estudio se realizó en la finca La Patasta, municipio La Sabana, departamento de Madriz, entre septiembre de 2004 y abril de 2005. El monitoreo se realizó semanalmente en cinco sitios específicos, mediante capturas manuales de especimenes, con ayuda de bolsas de plástico y vasos de vidrio con alcohol. Como resultado, se identificó y describió la fluctuación poblacional de insectos de las principales familias Scarabaeidae, Chrysomelidae, Curculionidae, Cantharidae, y de los órdenes Hemíptero (Cicadellidae, Pentatomidae, Miridae y Orthóptero (Acrididae y Tettigonidae. Igualmente, se identificó y describió la fluctuación poblacional de depredadores naturales de insectos de la familia Staphilinidae, Coccinelidae, Vespidae y Aracnidae, presentes en este cultivo.

  17. Chemical composition, antimicrobial and antioxidant activities of the essential oil of Tibetan herbal medicine Dracocephalum heterophyllum Benth.

    Science.gov (United States)

    Zhang, Chunjiang; Li, Hongyu; Yun, Tian; Fu, Yonghong; Liu, Chunmei; Gong, Bu; Neng, Benjia

    2008-01-10

    The essential oil of Tibetan medicine Dracocephalum heterophyllum Benth was obtained by hydrodistillation with a 0.7% (v/w) yield. The chemical composition of the essential oil was analyzed by gas chromatography-mass spectral (GC-MS). Eighty-three compounds, constituting about 89.83% of the total oil, were identified. The main compound in the oil were Cineole (14.89%), trans-nerolido (7.10%), 1-m-ethyl-2-(1-methylethyl)-benzene (4.42%), Germacrene-D (4.84%), Decahydro-1,1,4,7-tetramethyl-4aH-cycloprop[e]azulen-4a-ol (4.94%), p-menth-1-en-4-ol,acetate (4.34%), 4-methyl-1-(1-methylethyl)-3-cyclohexen-1-ol (4.10%). The antimicrobial activity of the oil was evaluated against nine bacterial, one yeast, and three fungi. The antimicrobial test result showed that the essential oil strongly inhibited the growth of test microorganisms studied. The maximal inhibition zones and MIC values for bacterial, yeast and fungi strain were in the range of 18-25 mm and 0.039-0.156 mg mL(-1); *20 mm, and 0.156 mg mL(-1); 8-24 mm and 0.313-2.5 mg mL(-1); respectively. The antioxidant activity of the oil was determined by the malonyldialdehyde (MDA) test, measuring the MDA concentration in mouse liver cell microsomal after induced lipid peroxidation using FeSO(4) and ascorbic acid, The inhibition of lipid peroxidation was 59.3% with a concentration of 0.5 mg mL(-1). Result presented here may suggest that the essential oil of D. heterophyllum posses antimicrobial and antioxidant properties, and therefore, they can be one of new medicinal resources for antimicrobial agent and/or used as a natural preservative ingredient in food and cosmetics and pharmaceuticals industry.

  18. Simultaneous Quantification of Limonin, Two Indolequinazoline Alkaloids, and Four Quinolone Alkaloids in Evodia rutaecarpa (Juss. Benth by HPLC-DAD Method

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    Pei-ting Zhang

    2013-01-01

    Full Text Available A simple and efficient HPLC-DAD (225 nm method was developed and validated for the simultaneous determination of limonin and six key alkaloids (evodiamine, rutaecarpine, 1-methyl-2-undecyl-4(1H-quinolone, evocarpine, 1-methy-2-[(6Z,9Z]-6,9-pentadecadienyl-4-(1H-quinolone, and dihydroevocarpine in Evodia rutaecarpa (Juss. Benth, which has been widely used as one of the Traditional Chinese Medicines. The chromatographic separation was carried out on a Hypersil BDS C18 column, and gradient elution was employed with a mobile phase containing acetonitrile and water. Contents of the analytes in 18 batches of samples were analyzed by ultrasonic extraction with ethanol and water mixture (80 : 20, v/v followed by HPLC analysis. Separation of the seven analytes was achieved within 60 min with good linearity (. The RSD of both the intraday and interday precision was below 1.85%. The accuracy at different concentrations was within the range of 97.91 to 100.49%. Hierarchical clustering analysis was performed to differentiate and classify the samples based on the contents of the seven constituents. This study indicated that the quality control of E. rutaecarpa could be simplified to the measurement of four constituents, and that limonin, 1-methyl-2-undecyl-4(1H-quinolone, and dihydroevocarpine should also be served as the chemical markers together with evodiamine for the quality control of Evodia rutaecarpa (Juss. Benth.

  19. In-vitro cytotoxicity study of methanolic fraction from Ajuga Bracteosa wall ex. benth on MCF-7 breast adenocarcinoma and hep-2 larynx carcinoma cell lines.

    Science.gov (United States)

    Pal, Akiriti; Toppo, Fedelic Aahish; Chaurasiya, Pradeep K; Singour, Pradeep K; Pawar, Rajesh S

    2014-01-01

    Ajuga bracteosa Wall ex Benth (Labiatae) is popularly known in India as Neelkanthi. A decoction of the leaves, flowers, and barks is used in India for the treatment of cancer including diabetes, malaria, and inflammation etc. The main objective of this study is to investigate the cytotoxic potential of Ajuga bracteosa. Successive solvent extraction of Ajuga bracteosa in petroleum ether, methanol, and water extracts was done. These extracts were tested against human breast adenocarcinoma (MCF-7) and larynx carcinoma (Hep-2) tumor cell lines, using the thiazolyl blue test (MTT) assay. The methanolic fraction of Ajuga bracteosa had shown the significant results against MCF-7 and Hep-2 tumor cell lines. The methanolic, petroleum ether and aqueous extract from Ajuga bracteosa, presented an IC50 value at 24 h of 10, 65, 70 μg/ml and 5, 30, 15 μg/ml on MCF-7 and Hep-2 cells, respectively. Steroids compounds namely β-sitosterol and unknown constituents were identified in the most active methanol extract of Ajuga bracteosa wall ex Benth. These known and unknown compounds exhibited cytotoxic potential against MCF-7 and Hep-2 cancer cells. Among all the tested extracts, methanolic extract can be considered as potential sources of anti-cancer compounds. Further studies are necessary for more extensive biological evaluations.

  20. Teores de Carbono Orgânico de Seis Espécies Naturais do Ecossistema da Floresta Ombrófi la Mista / Organic Carbon Contents in Six Native Species in the Araucaria Forest Ecosystem

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    Karla Simone Weber

    2006-08-01

    Full Text Available Muitas vezes o estoque de carbono de uma floresta é estimado com a aplicação de equações matemáticas que utilizam os teores de carbono para um determinado grupo de espécies. No entanto, isto só é possível se não houver diferenças significativas entre os teores de carbono destas espécies. Neste contexto, o presente trabalho visa analisar estatisticamente os teores de carbono de seis espécies nativas da Floresta Ombrófila Mista, na região sul do estado do Paraná. Foram feitas análises de variância entre os teores de carbono das espécies Myrsine ferruginea (Ruiz & Pav. Spreng. (capororoca, Ocotea porosa (Nees L. Barroso (imbuia, Mimosa scabrella Benth. (bracatinga, Styrax leprosus Hook & Arn. (carne-de-vaca, Symplocos unifl ora (Pohl Benth. (mariamole e Ilex paraguariensis St. Hil. (erva-mate para verificar se existe variação estatisticamente significativa entre espécies, entre as mesmas partes (casca, fuste, folhagem, galho vivo, galho morto e miscelânea de diferentes espécies e entre diferentes partes da mesma espécie. Os resultados revelam que a folhagem de Styrax leprosus foi à única que se diferenciou das demais, pois possui um baixo teor de carbono. Em todas as outras partes não houve diferenças em função da espécie. A análise entre partes da mesma espécie, revelou que existem padrões diferentes para cada espécie, mas que a porção folhagem sempre concentra os maiores teores de carbono. Desconsiderando a separação em diferentes partes, não houve diferença estatística entre os teores de carbono quando comparados os valores médios de cada espécie.

  1. Parâmetros bioquímicos foliares das espécies Licania tomentosa (Benth. e Bauhinia forficata (Link. para avaliação da qualidade do ar Foliar analyses of biochemical parameters of Licania tomentosa (Benth. and Bauhinia forficata (Link. species for air quality assessment

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    Otávio Luiz Gusso Maioli

    2008-01-01

    Full Text Available Foliar analysis of biochemical parameters were carried out in order to investigate the influence of air pollutants on two tropical tree species (Licania tomentosa (Benth. and Bauhinia forfícata (Link.. Special attention was given to tropospheric ozone due to the fact that concentration levels in the region were found to be up to 140 µg m-3 for a 4 h average time, which is well above the value that can cause injuries to orchides and tobacco (59 µg m-3. Other pollutants such as nitrogen and sulphur oxides were measured and their ambient concentrations were also associated to biochemical alterations in the investigated species.

  2. Phytotoxic Effects of Nepeta meyeri Benth. Extracts and Essential Oil on Seed Germinations and Seedling Growths of Four Weed Species

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    Saban Kordali

    2015-05-01

    Full Text Available Essential oil isolated from the aerial parts of Nepeta meyeri Benth. by hydrodistilation was analysed by GC and GC-MS methods. A total 18 components were identified in the oil representing 100.0% of the oil. Main components were 4aα,7α,7aβ-nepetalactone (80.3%, 4aα,7α,7aα–nepetalactone (10.3%, trans-pulegol (3.1%, 1, 8-cineole (3.0% and β-bourbonene (2.0%. In addition, n-hexane extract of N. meyeri was analysed by using GC and GC-MS methods and 18 components were identified. Likewise, nepetalactones, 4aα,7α,7aβ-nepetalactone (83.7%, 4aα,7α,7aα–nepetalactone (3.6%, 1, 8-cineole (1.9% and α-terpinene (1.5% were the predominat compounds in the hexane extract. Three concentrations (0.5, 1.0 and 2.0 mg/mL of the essential oil and n-hexane, chloroform, acetone and methanol extracts isolated from the aerial partsand roots were tested for the herbicidal effects on the germination of the seeds of four weed species including Amaranthus retroflexus L., Chenopodium album L., Cirsium arvense L. and Sinapsis arvensis L. The essential oil of N. meyeri completely inhibited the germination of all weed seeds whereas the extracts showed various inhibition effects on the germination of the weed species. Herbicidal effect was increased with the increasing application concentrations of the extracts. In general, the acetone extract was found to be more effective as compared to the other extracts. All extracts also exhibited various inhibition effects on the seedling growths of the weed species. All extracts also tested for their phytotoxic effects on the weeds at greenhouse condition and the results showed that the oil and extracts caused mortality with 22.00-66.00% 48h after the treatments. These findings suggest that the essential oil and the extracts of N. meyeri have potentials for use as herbicides against those weed species.

  3. Liver morphology and morphometry and plasma biochemical parameters of Wistar rats that received leaf infusion of Rudgea viburnoides Benth. (Rubiaceae

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    Juliana Castro Monteiro

    2009-04-01

    Full Text Available Rudgea viburnoides leaves are widely used in popular Brazilian medicine as a diuretic, antirheumatic, hypotensive and blood depurative tea. The present study was carried out to investigate the effects of this infusion on the liver and on the plasma biochemical parameters of Wistar rats. Two groups received the R. viburnoides leaf infusion at a daily dose of 10 or 20g dry-leaves/L water, during 40 days. The histopathological analysis did not show degenerated areas or infiltration of leucocytes. Hepatic morphometry showed accumulation of fat in the hepatocytes of the treated groups. There was no significant change in the plasma levels of urea, creatinin, uric acid, direct bilirubin, cholesterol, total proteins, albumin, gamma glutamyl tranferase (gamma-GT, alanine transaminase (ALT, aspartate transaminase (AST, chlorine, phosphate and calcium. A significant reduction in the plasma levels of triacylglycerol (TAG occurred in the group that received the higher dose.As folhas de Rudgea viburnoides Benth. são utilizadas na medicina popular como diuréticas, hipotensoras, anti-reumáticas, depurativas do sangue e em regimes de emagrecimento. O presente estudo foi delineado para avaliar o efeito da infusão das folhas de R. viburnoides nos parâmetros bioquímicos plasmáticos e na morfologia e morfometria hepática de ratos Wistar adultos. Dois grupos receberam a infusão das folhas, diariamente, nas dosagens de 10 e 20 g de folhas secas/L de água, durante 40 dias. O grupo controle recebeu a mesma quantidade de água. As análises histopatológicas não mostraram áreas degeneradas e infiltrados inflamatórios. A morfometria hepática mostrou acúmulo significativo de gordura nos hepatócitos dos animais tratados, principalmente no grupo que recebeu a maior dose da infusão (8,75% de gotículas lipídicas, comparado com 0,25% delas encontradas nos animais controles. Não foram observadas alterações nos níveis plasmáticos de uréia, creatinina,

  4. Actividad antiparasitaria in vitro de extractos etanólicos de Erythrina crista-galii L., Erythrina falcata Benth y Erythrina dominguezii Hassl

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    Gloria Yaluff

    2015-08-01

    Full Text Available La tripanosomiasis americana y la leishmaniosis son problemas de salud pública relevantes en Iberoamérica. Se evaluó la actividad antiparasitaria in vitro de extractos de cortezas y hojas de Erythrina crista-galli L., E. dominguezii Hassl y E. falcata Benth sobre formas tripomastigotas de Trypanosoma cruzi y promastigotas de Leishmania amazonensis, L. infantum L. braziliensis. El extracto de corteza de E. dominguezii Hassl presentó CI50 de 11,7 μg/ml y 14,9 μg/ml con dos cepas de T. cruzi, Ypsilon y Cl Brener. El extracto de hoja presentó CI50 mayor a 250 μg/ml. La corteza de E. falcata Benth tuvo CI50 de 11,29 μg/ml y 7,37 μg/ml con Ypsilon y Cl Brener y el extracto de hojas CI50 de 37,2 μg/ml y 25,68 μg/ml. El extracto de hojas de E. crista-galli L. presentó CI50 mayor a 250 μg/ml, y el de corteza CI50 11,2μg/ml y 7,1μg/ml con Ypsilon y Cl Brener. El extracto de corteza de E. dominguezii Hassl presentó CI50 de 3,22 μg/ml, 6,94 μg/ml y 1,9 μg/ml para L. amazonensis, L. infantum y L. braziliensis y en hojas la CI50 fue mayor a 100 μg/ml. La corteza de E. crista-galli L. tuvo CI50 de 2,1 μg/ml, 2,09 μg/ml y 1,6 μg/ml para las tres especies. El extracto de hoja tuvo una CI50 mayor a 100 μg/ml. El extracto de corteza de E. falcata Benth tuvo CI50 de 2,17 μg/ml, 2,09 μg/ml y 1,52 μg/ml con las tres especies de Leishmania y el de hoja CI50 de 14,9 μg/ml, 10,4 μg/ml y 11,6 μg/ml. Los extractos de corteza podrían ser una alternativa para el tratamiento de estas enfermedades parasitarias, previa confirmación con estudios in vivo y de toxicidad.

  5. Estabelecimento e desenvolvimento de Dalbergia miscolobium Benth. em duas fitofisionomias típicas dos cerrados do Brasil Central Establishment and development of Dalbergia miscolobium Benth. in two typical savanna physiognomies of Central Brazil

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    Vívian S. Braz

    2000-04-01

    Full Text Available As formações savânicas do Brasil Central caracterizam-se por uma camada rasteira contínua, em que predominam gramíneas, entrecortada por uma camada de arbustos e árvores de densidade variável. Dalbergia miscolobium Benth. é uma leguminosa arbórea típica dos cerrados do Planalto Central. Este estudo examinou os efeitos da seca, disponibilidade de luz, herbivoria e fogo no estabelecimento e crescimento dessa espécie no campo sujo (estrato herbáceo contínuo com árvores e arbustos esparsos e no cerrado sensu stricto, onde a vegetação arbustivoarbórea é mais densa. Mudas foram transplantadas nas duas fitofisionomias, sendo acompanhadas mensalmente as variações na altura, produção de folhas e percentagem da área foliar removida por herbivoria, ao longo de cinco anos (1993-1998. A mortalidade ocorreu principalmente durante a época chuvosa. A seca sazonal não foi um importante fator de mortalidade. Número maior de mudas sobreviveu no campo sujo. Estas mudas não foram mortas por uma queimada ao final da seca de 1994, enquanto várias morreram no cerrado. O crescimento da parte aérea foi lento, em torno de 2,2cm/ano no campo sujo e 3,1cm/ano no cerrado, ocorrido principalmente na estação chuvosa. A maioria das mudas apresentou lesões necróticas de cor preta nas folhas, causada pelo ataque do fungo Phoma sp. A queda das folhas ocorreu durante a estação seca e o número máximo de folhas foi alcançado do meio para o final da época chuvosa, sempre seguido por um pico na herbivoria. Dados de disponibilidade de luz sugerem que plantas com folhas horizontais a 5cm do solo, atingiriam em média apenas 32% da capacidade máxima de assimilação de CO2, devido ao sombreamento. Entretanto, aumento da altura da planta de 5 para 50cm, onde não há mais sombreamento do estrato herbáceo, dobraria os valores estimados de assimilação de CO2 Assim sendo, o desenvolvimento desta espécie seria limitado pela disponibilidade de água e

  6. Antibacterial Activity of Indian Borage (Plectranthus amboinicus Benth) Leaf Extracts in Food Systems and
Against Natural Microflora in Chicken Meat.

    Science.gov (United States)

    Gupta, Sandeep Kumar; Negi, Pradeep Singh

    2016-03-01

    The ability of acetone and ethyl acetate extracts of the leaves of a traditional Indian medicinal plant, Indian borage (Plectranthus amboinicus Benth) to prevent spoilage of artificially inoculated model food systems (cabbage and papaya) and natural microflora of chicken meat was evaluated. These extracts were able to reduce the bacterial counts in all food systems; however, the effective concentration varied with the complexity of the system (cabbage

  7. The Main Chemical Composition and in vitro Antifungal Activity of the Essential Oils of Ocimum basilicum Linn. var. pilosum (Willd. Benth

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    Ji-Wen Zhang

    2009-01-01

    Full Text Available The essential oils of the aerial parts of Ocimum basilicum Linn.var. pilosum (Willd. Benth., an endemic medicinal plant growing in China, was obtained by hydrodistillation and analysed by GC-MS. Fifteen compounds, representing 74.19% of the total oil were identified. The main components were as follows: linalool (29.68%, (Z-cinnamic acid methyl ester (21.49%, cyclohexene (4.41%, α- cadinol (3.99%, 2,4-diisopropenyl-1-methyl-1-vinylcyclohexane (2.27%, 3,5-pyridine-dicarboxylic acid, 2,6-dimethyl-diethyl ester (2.01%, β-cubebene (1.97%, guaia-1(10,11-diene (1.58%, cadinene (1.41% (E-cinnamic acid methyl ester (1.36% and β-guaiene (1.30%. The essential oils showed significant antifungal activity against some plant pathogenic fungi.

  8. Study of evolution and developmental mechanism of stamen in Salvia hypoleuca Benth. (Lamiaceae and related taxa using Electron Microscopy and molecular phylogenetic tools

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    Maryam-Sadat Asadollahi

    2014-11-01

    Full Text Available The genus Salvia L. includes a group of plants having two stamens with a special morphology. Natural groups within this genus consist of species with special type of stamen morphology. In this paper, phylogenetic placement and developmental stages of stamen of S. hypoleuca Benth. were studied. Examination of this and related species showed that each flower had two stamens with posterior thecae not expressed and the distal posterior ends of the adjacent connectives were fused into a complex structure blocking access to nectar. All species with such type of stamens grouped into a monophyletic clade with robust support. Study of stamen development in S. hypoleuca showed that normal, anterior thecae were evolved before the other organs and that the stamen found its final matured form before flower opened. In an opened flower, stamen and reproductive organs could be found in their final maturestage. In addition, it seemed that stamen development in related species should be similar.

  9. Coleus barbatus Benth and Ocimum basilicum L. (Lamiaceae, New Host Plants to Spodoptera cosmioides (Walker (Lepidoptera: Noctuidae in Sinop, State of Mato Grosso, Brazil

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    Evaldo Pires

    2014-04-01

    Resumo. Coleus barbatus Benth e Ocimum basilicum L. são espécies de plantas comumente utilizadas com fins medicinais e gastronômicos, respectivamente. Lagartas do gênero Spodoptera são generalistas devido à ampla variedade de plantas que utilizam como recurso alimentar. O objetivo desta pesquisa foi registrar a ocorrência de Spodoptera cosmioides (Walker (Lepidoptera: Noctuidae no município de Sinop, Mato Grosso, Brasil, e ainda, relatar C. barbatus e O. basilicum como potenciais plantas hospedeiras para esta espécie de inseto. Recomenda-se ainda a inclusão de S. cosmioides em monitoramentos visando o Manejo Integrado de Pragas (MIP nestas plantas.

  10. CARACTERIZAÇÃO MORFOANATOMIA E HISTOQUÍMICA DE Hyptis rubicunda POHL ex BENTH. (LAMIACEAE), OCORRENTE NA SERRA DOURADA, GOIÁS, BRASIL

    OpenAIRE

    Silva de Castro, Aline; Dias Ferreira, Heleno; Rezende, Maria Helena; Faria, Maria Tereza

    2015-01-01

    As Lamiaceae são reconhecidas por sua importância econômica e etnofarmacológica. O gênero Hyptis pertence às Lamiaceae com cerca de 400 espécies, no Brasil são encontradas 202 espécies, sendo 146 endêmicas. O objetivo desse estudo foi caracterizar morfologicamente e anatomicamente o caule e a folha da espécie Hyptis rubicunda Pohl ex Benth., visando auxiliar na caracterização desta espécie e contribuir com novos dados para o grupo. A espécie foi coletada na Serra Dourada, Mossâmedes, Goiás. P...

  11. Chemical Composition, and Antibacterial (against Staphylococcus aureus and Free-Radical-Scavenging Activities of the Essential Oil of Scrophularia amplexicaulis Benth.

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    Ardalan Pasdaran

    2012-07-01

    Full Text Available Chemical composition of the essential oil obtained from the aerial parts of Scrophularia amplexicaulis Benth. was analyzed, for the first time, by the gas chromatography/mass spectrometry (GC-MS and gas chromatography/flame ionization detection (GC-FID. A total yield of 3 mg of essential oil per100 g of plant dry mass was obtained, and 27 compounds were identified, representing 97. 7 % of total oil. The essential oil were characterized by a high content of oxygenated monoterpenes and phenolic derivatives. The main constituents were eugenol (53.8%, eugenol acetate (24.5%, b -caryophyllene (5.7%, caryophyllene oxide (6.4% and aromadendrene oxide II (2.1%. The antimicrobial activity of the essential oil was tested against Staphylococcus aureus using the well diffusion method, and t he free-radical-scavenging activity was assessed by the 2,2-diphenyl-picryl-hydrazyl (DPPH assay.

  12. Strong Polarity Components of Pogostemon cablin(Blance)Benth.%广藿香大极性化学成分的研究

    Institute of Scientific and Technical Information of China (English)

    丁文兵; 刘梅芳; 魏孝义; 林立东

    2009-01-01

    从广藿香(Pogostemon cablin (Blance)Benth.)地上部分乙醇提取物的正丁醇萃取部位分离得到13个化合物.通过光谱和波谱分析,分别鉴定为:芹菜素(1)、3,5,4'-三羟基-7-甲氧基黄酮(2)、3,5-二羟基_4',7-二甲氧基黄酮(3)、Apigenin 7-galacturonide(4)、Apigenin 7-(O-methylghacuronide)(5),Luteolin 7-O-(6-O-methyl-β-D-glucuronopyranoside)(6),4',5-二羟基-3',7-二甲氧基二氢黄酮(7)、Quercetha-7-β-D-ghcoside(8),3,23-Dihydroxy-12-oleanen-28-oic acid(9)、Syringaresinol-β-D-glucoside(10),毛蕊花糖苷(11)、列当苷(12)、紫葳新苷(13),化合物2~13均为首次从该植物中分离得到.%Thirteen compounds were isolated from n-butanol extraction of ethanol extract of the aerial parts of Pogostemon cablin (Blance) Benth. On the basis of spectral data,they were identified as apigenin (1),3,5,4'-Trihydroxy-7-methoxyflavone (2),3,5-Dihydroxy-4',7-dimethoxyflavone (3),apigenin 7-galacturonide(4),apigenin 7-(O-methylghcuronide)(5),luteolin 7-O-(6-O-methyl-β-D-glucuronopyranoside)(6),4',5-Dihydroxy-3',7-dimethoxyflavanone (7),quercetin-7-β-D-glucoside (8),3,23-dihydroxy-12-oleanen-28-oic acid (9),syringaresinolβ-D-glucoside(10),verbascoside(11),orobanchoside(12),campneoside Ⅰ(13).Compounds 2~13 were isolated from the plant for the first time.

  13. Verificação da atividade antibacteriana de sabonete líquido contendo extrato glicólico de Dimorphandra mollis Benth.

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    KETYLIN FERNANDA MIGLIATO

    2009-12-01

    Full Text Available Dimorphandra mollis Benth., Compositae, falsobarbatimão, é utilizada topicamente como cicatrizante, adstringente e antimicrobiano. No presente estudo, verificou-se a atividade antibacteriana de sabonete líquido contendo extrato glicólico de D. mollis (EGD em diferentes concentrações (8, 15 e 20% e em diferentes pHs (6 e 8. Foram preparadas cinco formulações (F de sabonete: F1 - triclosan (0,1%, F2 - EGD (8%, F3 - EGD (15%, F4 - EGD (20% e F5 - sem conservante. Cascas de D. mollis foram secas em estufa de ar circulante e pulverizadas. Os extratos brutos foram preparados por turbo-extração utilizando-se etanol. Após filtração, os extratos foram concentrados em evaporador rotatório, liofilizados e ressuspendidos em propilenoglicol para a obtenção do extrato glicólico. A atividade antibacteriana foi verificada pelo método de difusão em ágar, empregando cilindros em placa. Placas contendo Staphylococcus aureus, Pseudomonas aeruginosa e Escherichia coli foram incubadas a 37ºC durante 24 horas. Após incubação, as leituras foram realizadas com paquímetro, observando-se o diâmetro do halo de inibição de crescimento bacteriano. Verificouse que o sabonete líquido contendo triclosan provocou inibição do crescimento bacteriano em ambos os pHs; já os sabonetes sem conservante e contendo EGD, independente da concentração e do pH empregados, não apresentaram atividade antibacteriana. Palavras-chave: Atividade antibacteriana. Sabonete líquido. Dimorphandra mollis Benth.

  14. Padrão de resposta de Mimosa pudica e Senna obtusifolia à atividade potencialmente alelopática de espécies de Poaceae Response pattern of Mimosa pudica e Senna obtusifolia to potentially allelopathic activity of Poaceae species

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    A.P.S. Souza Filho

    2010-01-01

    Full Text Available A alelopatia é um importante mediador de interferências que alteram a dinâmica de espécies de plantas em sistemas agrícolas. Neste trabalho, procurou-se determinar a existência de padrão de respostas das plantas daninhas Mimosa pudica e Senna obtusifolia a espécies da família Poaceae, via interação entre espécies e frações e ordenamento dos efeitos. Bioensaios de germinação de sementes e alongamento da radícula e do hipocótilo foram desenvolvidos, em condições controladas. Os testes foram realizados utilizando-se extratos hidroalcoólicos na concentração de 1,0% das frações folha, raiz e sementes de quatro espécies de Poaceae. Diferenças na intensidade dos efeitos para os fatores espécie e fração foram verificadas. O padrão de atividade observado foi de Paspalum maritimum apresentar as inibições mais intensas. Entre as espécies de Brachiaria, as inibições mais intensas foram produzidas por B. brizantha. Houve resposta efetiva para a especificidade entre espécies e fração para a germinação e alongamento da radícula, especialmente na espécie Mimosa pudica. Apenas para os extratos de P. maritimum foram observadas especificidade e efetividade em relação à germinação de sementes e alongamento da radícula da espécie Senna obtusifolia. A fração folha foi mais efetiva nas inibições, sobretudo na espécie P. maritimum. Comparativamente, a espécie Mimosa pudica foi mais sensível aos efeitos dos extratos, especialmente em relação ao desenvolvimento da radícula e do hipocótilo. A intensidade dos efeitos alelopáticos variou na seguinte ordem: alongamento da radícula > germinação de sementes > alongamento do hipocótilo. A ordenação dos resultados, para os indicadores de inibição, indicou discriminação no padrão para as espécies receptoras, o que sugere especificidade dos extratos.Allelopathy is an important device to measure interferences affecting the dynamics of plant species in

  15. Vasorelaxant effect of Hyptis fruticosa Salzm. ex Benth., Lamiaceae, dichloromethane extract on rat mesenteric artery Efeito vasorelaxante do extrato diclorometano de Hyptis fruticosa Salzm. ex Benth., Lamiaceae, em artéria mesentérica de ratos

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    Ítalo J. A. Moreira

    2010-11-01

    Full Text Available Vasorelaxant effect of Hyptis fruticosa dichloromethane extract (HFDE on isolated rings of rat mesenteric artery was evaluated in this study. In intact rings, HFDE (0.1-3000 µg/mL induced concentration-dependent vasorelaxations (Emax = 119±14%; n = 6 of phenylephrine tonus that were not modified after endothelium removal (Emax = 116±6%; n = 6, after KCl 20 mM (Emax = 135±9%; n = 6 or in rings pre-contracted with KCl 80 mM (Emax = 125±4%; n = 6. In endothelium denuded rings, HFDE (300 or 1000 µg/mL inhibited contractions induced by CaCl2 (maximal inhibition = 25±7% and 95±1%; respectively. Furthermore, HFDE promoted an additional vasorelaxation (15±3%; n = 7 after maximal response of 10 µM nifedipine (78±3%; n = 7. In conclusion, HFDE induces vasorelaxant effect through an endothelium-independent pathway, which appears to be due in major part to inhibition of the Ca2+ influx through voltage-operated Ca2+ channels.O efeito vasorelaxante do extrato diclorometano de Hyptis fruticosa Salzm. ex Benth., Lamiaceae (HFDE, em anéis isolados de artéria mesentérica de ratos foi avaliado nesse estudo. Em anéis intactos, pré-contraídos com fenilefrina (10 µM, HFDE (0,1-3000 µg/mL induziu vasorelaxamento de maneira dependente de concentração (Emax = 119±14%; n = 6, o qual não foi afetado após remoção do endotélio (Emax = 116±6%; n = 6, após KCl 20 mM (Emax = 135±9%; n = 6 ou em anéis pré-contraídos com KCl 80 mM (Emax = 125±4%; n = 6. Em anéis sem endotélio, HFDE (300 ou 1000 µg/mL inibiu as contrações induzidas por CaCl2 (inibição máxima = 25±7% e 95±1%, respectivamente. Além disso, HFDE promoveu um vasorelaxamento adicional (15±3%; n = 7 sobre o relaxamento máximo de 10 µM de nifedipina (78±3%, n = 7. Em conclusão, HFDE induz efeito vasorelaxante através de uma via independente de endotélio, possivelmente devido à inibição do influxo de Ca2+ através de canais de Ca2+ operados por voltagem.

  16. Determination of N,N-dimethyltryptamine in Mimosa tenuiflora inner barks by matrix solid-phase dispersion procedure and GC-MS.

    Science.gov (United States)

    Gaujac, Alain; Aquino, Adriano; Navickiene, Sandro; de Andrade, Jailson Bittencourt

    2012-01-15

    N,N-dimethyltryptamine (DMT) is a potent hallucinogen found in beverages consumed in religion rituals and neo-shamanic practices over the world. Two of these religions, Santo Daime and União do Vegetal (UDV), are represented in countries including Australia, the United States and several European nations. In some of this countries there have been legal disputes concerning the legalization of ayahuasca consumption during religious rituals, a beverage rich in DMT. In Brazil, even children and pregnant women are legally authorized to consume ayahuasca in a religious context. A simple and low-cost method based on matrix solid-phase dispersion (MSPD) and gas chromatography with mass spectrometric detection (GC-MS) has been optimized for the determination of N,N-dimethyltryptamine in Mimosa tenuiflora inner bark. The experimental variables that affect the MSPD method, such as the amounts of solid-phase and herbal sample, solvent nature, eluate volume and NaOH concentration were optimized using an experimental design. The method showed good linearity (r = 0.9962) and repeatability (RSD < 7.4%) for DMT compound, with detection limit of 0.12 mg/g. The proposed method was used to analyze 24 samples obtained locally. The results showed that concentrations of the target compound in M. tenuiflora barks, ranged from 1.26 to 9.35 mg/g for these samples. Copyright © 2011 Elsevier B.V. All rights reserved.

  17. Inoculation of Mimosa latispinosa Lam with the Commercial Arbuscular Mycorrhizal Fungus Rhizophagus irregularis DAOM 197198, and Bradyrhizobium spp. Under Nursery Production Conditions in South-East Madagascar

    Directory of Open Access Journals (Sweden)

    Sarasin, G.

    2017-01-01

    Full Text Available Qit Madagascar Minerals (QMM has planned several actions to reduce the environmental footprint of its mining project located near the city of Fort-Dauphin (Madagascar. One of these actions is the reclamation of a portion of its mined sites. Different symbiotic strains were tested as bio-enhancers for the ecological restoration using Mimosa latispinosa Lam, a native and pioneer shrub. The symbiotic strains tested in nursery were the commercial strain of arbuscular mycorrhizal fungus, Rhizophagus irregularis DAOM197198, and two local strains of Bradyrhizobium spp., STM1415 and STM1447, inoculated alone or dually with the arbuscular mycorrhiza. Treatments did not significantly increase the plant height and dry mass. However, plants grown in tyndallized soil had better growth than those in unsterilized soil. Results obtained twenty weeks after inoculation suggest that soil tyndallization (heating at 100°C and at atmospheric pressure of 700 kPa during three hours is an effective method for nursery production of high quality seedlings of M. latispinosa.

  18. Assessing Chemical Constituents of Mimosa caesalpiniifolia Stem Bark: Possible Bioactive Components Accountable for the Cytotoxic Effect of M. caesalpiniifolia on Human Tumour Cell Lines

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    Nayana Bruna Nery Monção

    2015-03-01

    Full Text Available Mimosa caesalpiniifolia is a native plant of the Brazilian northeast, and few studies have investigated its chemical composition and biological significance. This work describes the identification of the first chemical constituents in the ethanolic extract and fractions of M. caesalpiniifolia stem bark based on NMR, GC-qMS and HRMS analyses, as well as an assessment of their cytotoxic activity. GC-qMS analysis showed fatty acid derivatives, triterpenes and steroid substances and confirmed the identity of the chemical compounds isolated from the hexane fraction. Metabolite biodiversity in M. caesalpiniifolia stem bark revealed the differentiated accumulation of pentacyclic triterpenic acids, with a high content of betulinic acid and minor amounts of 3-oxo and 3β-acetoxy derivatives. Bioactive analysis based on total phenolic and flavonoid content showed a high amount of these compounds in the ethanolic extract, and ESI-(−-LTQ-Orbitrap-MS identified caffeoyl hexose at high intensity, as well as the presence of phenolic acids and flavonoids. Furthermore, the evaluation of the ethanolic extract and fractions, including betulinic acid, against colon (HCT-116, ovarian (OVCAR-8 and glioblastoma (SF-295 tumour cell lines showed that the crude extract, hexane and dichloromethane fractions possessed moderate to high inhibitory activity, which may be related to the abundance of betulinic acid. The phytochemical and biological study of M. caesalpiniifolia stem bark thus revealed a new alternative source of antitumour compounds, possibly made effective by the presence of betulinic acid and by chemical co-synergism with other compounds.

  19. Biodiversity of Mimosa pudica rhizobial symbionts (Cupriavidus taiwanensis, Rhizobium mesoamericanum) in New Caledonia and their adaptation to heavy metal-rich soils.

    Science.gov (United States)

    Klonowska, Agnieszka; Chaintreuil, Clémence; Tisseyre, Pierre; Miché, Lucie; Melkonian, Rémy; Ducousso, Marc; Laguerre, Gisèle; Brunel, Brigitte; Moulin, Lionel

    2012-09-01

    Rhizobia are soil bacteria able to develop a nitrogen-fixing symbiosis with legumes. They are taxonomically spread among the alpha and beta subclasses of the Proteobacteria. Mimosa pudica, a tropical invasive weed, has been found to have an affinity for beta-rhizobia, including species within the Burkholderia and Cupriavidus genera. In this study, we describe the diversity of M. pudica symbionts in the island of New Caledonia, which is characterized by soils with high heavy metal content, especially of Ni. By using a plant-trapping approach on four soils, we isolated 96 strains, the great majority of which belonged to the species Cupriavidus taiwanensis (16S rRNA and recA gene phylogenies). A few Rhizobium strains in the newly described species Rhizobium mesoamericanum were also isolated. The housekeeping and nod gene phylogenies supported the hypothesis of the arrival of the C. taiwanensis and R. mesoamericanum strains together with their host at the time of the introduction of M. pudica in New Caledonia (NC) for its use as a fodder. The C. taiwanensis strains exhibited various tolerances to Ni, Zn and Cr, suggesting their adaptation to the specific environments in NC. Specific metal tolerance marker genes were found in the genomes of these symbionts, and their origin was investigated by phylogenetic analyses. © 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

  20. Applying behavioral-ecological theory to plant defense: light-dependent movement in Mimosa pudica suggests a trade-off between predation risk and energetic reward.

    Science.gov (United States)

    Jensen, Evelyn L; Dill, Lawrence M; Cahill, James F

    2011-03-01

    Many animal species tolerate different amounts of predation risk based on environmental conditions and the individual's own condition, often accepting greater risk when energetically stressed. We studied the sensitive plant Mimosa pudica to see whether it too accepts greater risk of predation when less light energy is available. This plant displays a defensive behavior of rapidly folding its leaves when stimulated by touch, thereby decreasing visibility to herbivores. Averting herbivory involves a trade-off because leaf closure results in a reduction in light foraging. We manipulated the light environment of individual M. pudica plants and recorded the time it took a plant to reopen its leaves following stimulation as a measure of tolerance of predation risk. As predicted by theory, avoidance behavior was sustained longer under high light conditions than under more light-limited conditions. These findings suggest this species balances the risk and reward of antiherbivore behavior in relation to current environmental conditions and that behavioral-ecological theory is a useful framework for understanding plant responses to predators.

  1. Early changes in membrane permeability, production of oxidative burst and modification of PAL activity induced by ergosterol in cotyledons of Mimosa pudica.

    Science.gov (United States)

    Rossard, Stéphanie; Luini, Estelle; Pérault, Jean-Michel; Bonmort, Janine; Roblin, Gabriel

    2006-01-01

    Ergosterol (a fungal membrane component) was shown to induce transient influx of protons and membrane hyperpolarization in cotyledonary cells of Mimosa pudica L. By contrast, chitosan (a fungal wall component with known elicitor properties) triggered membrane depolarization. In the processes induced by ergosterol, a specific desensitization was observed, since cells did not react to a second ergosterol application but did respond to a chitosan treatment. This comparative study correspondingly shows that ergosterol and chitosan were perceived in a distinct manner by plant cells. Generation of O2*-, visualized by infiltration with nitroblue tetrazolium, was displayed in organs treated with ergosterol and chitosan. This AOS production was preceded by an increase in activity of NADPH oxidase measured in protein extracts of treated cotyledons. In all the previously described processes, cholesterol had no effect, thereby indicating that ergosterol specifically induced these physiological changes known to participate in the reaction chain activated by characteristic elicitors. Contrary to chitosan, ergosterol did not greatly activate secondary metabolism as shown by the small change in content of free phenolics and by the low modification in activity of PAL, the key enzyme of this metabolic pathway. Therefore, future studies have to clarify the signalling cascade triggered by ergosterol recognition.

  2. Structural characterization of a novel derivative of myricetin from Mimosa pudica as an anti-proliferative agent for the treatment of cancer.

    Science.gov (United States)

    Jose, Joby; Dhanya, A T; Haridas, Karickal R; Sumesh Kumar, T M; Jayaraman, Sony; Variyar, E Jayadevi; Sudhakaran, Sudheesh

    2016-12-01

    The study was initiated to determine the anticancer activity of a novel compound isolated from the plant Mimosa pudica. The structure of the compound was identified as a derivative of myricetin having alkyl, hydroxy alkyl and methyl substitutions on the basis of spectral evidences (UV-vis, FT-IR, (1)H NMR and Mass spectra). The isolated compound was interpreted as 2-(2',6'-dimethyl-3',4',5'-alkyl or hydroxy alkyl substituted phenyl)-3-oxy-(alkyl or hydoxy alkyl)- 5,7-dihydroxy-chromen-4-one. In vitro evaluation of anticancer activity against human lung adenocarcinoma cell line (A549) and human erythroleukemic cell line (K562) were conducted using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. In vivo anticancer activity was determined against Dalton's Ascites Lymphoma (DAL) in Swiss albino mice. The mice were treated with intraperitoneal administration of the compound at 25mg/kg and 100mg/kg body weight and were compared with the normal, DAL control and standard drug cyclophosphamide treated groups. The histology revealed that the compound could protect the cellular architecture of liver and kidney. The results from the in vitro, in vivo and histological examinations confirmed the ethnopharmacological significance of the isolated compound and could be considered further for the development of an effective drug against cancer. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  3. The geographical patterns of symbiont diversity in the invasive legume Mimosa pudica can be explained by the competitiveness of its symbionts and by the host genotype.

    Science.gov (United States)

    Melkonian, Rémy; Moulin, Lionel; Béna, Gilles; Tisseyre, Pierre; Chaintreuil, Clémence; Heulin, Karine; Rezkallah, Naïma; Klonowska, Agnieszka; Gonzalez, Sophie; Simon, Marcelo; Chen, Wen-Ming; James, Euan K; Laguerre, Gisèle

    2014-07-01

    Variations in the patterns of diversity of symbionts have been described worldwide on Mimosa pudica, a pan-tropical invasive species that interacts with both α and β-rhizobia. In this study, we investigated if symbiont competitiveness can explain these variations and the apparent prevalence of β- over α-rhizobia. We developed an indirect method to measure the proportion of nodulation against a GFP reference strain and tested its reproducibility and efficiency. We estimated the competitiveness of 54 strains belonging to four species of β-rhizobia and four of α-rhizobia, and the influence of the host genotype on their competitiveness. Our results were compared with biogeographical patterns of symbionts and host varieties. We found: (i) a strong strain effect on competitiveness largely explained by the rhizobial species, with Burkholderia phymatum being the most competitive species, followed by B. tuberum, whereas all other species shared similar and reduced levels of competitiveness; (ii) plant genotype can increase the competitiveness of Cupriavidus taiwanensis. The latter data support the likelihood of the strong adaptation of C. taiwanensis with the M. pudica var. unijuga and help explain its prevalence as a symbiont of this variety over Burkholderia species in some environments, most notably in Taiwan. © 2013 Society for Applied Microbiology and John Wiley & Sons Ltd.

  4. Two myrmecophilous scale insects, Cryptostigma urichi (Cockerell (Hemiptera, Coccidae and Farinococcus multispinosus Morrison (Hemiptera, Pseudococcidae, cohabiting inside branches of Anadenanthera falcata (Benth. Speg. (Fabales, Fabaceae in the Cerrado area of São Paulo State, Brazil

    Directory of Open Access Journals (Sweden)

    André R. Nascimento

    2012-12-01

    Full Text Available The soft scale Cryptostigma urichi (Cockerell, 1894 and the mealybug Farinococcus multispinosus Morrison, 1922 are reported cohabiting inside branches of Anadenanthera falcata (Benth. Speg. (Angico-preto, both tended by the ant Azteca oecocordia Longino, 2007 (Hymenoptera, Formicidae. This interaction was observed in the Cerrado region sensu stricto, at the Federal University of São Carlos, UFSCar, Brazil. A. oecocordia is recorded for the first time in Brazil.

  5. 湖北野生香茶菜花期挥发油GC—MS分析%Analysis of Volatile Oil of Hubei Wild Rabdosia Amethystoides (Benth.) H. Hara in the Flower Period by GC-MS

    Institute of Scientific and Technical Information of China (English)

    梁利香; 陈琼; 陈利军

    2015-01-01

    目的:研究香茶菜花期挥发油的化学成分.方法:采用水蒸汽蒸馏法提取香茶菜花期的挥发油,以气相色谱—质谱联用(GC-MS)仪测定挥发油化学组分.结果:主要成分为2-甲氧基-4-乙烯基苯酚(33.076%),1,2,3,4-四甲基-5-亚甲基-1,3-环戊二烯(13.826%),2,3,4,6-四甲基苯酚(11.426%).结论:香茶菜含大量常用食品添加剂2-甲氧基-4-乙烯基苯酚,可以综合开发利用.%Objective:Studies on the chemical constituents of flower volatile oil from Rabdosia amethystoides (Benth.)H. Hara. Method:The essential oil was extracted from Rabdosia amethystoides (Benth.)H.Hara.by steam distillation. Compon-ents of the essential oils were analyzed by GC-MS. Result:The main chemical components are 2-Methoxy-4-vinylphenol (33.076%), 1,3-Cyclopentadiene, 1,2,3,4-tetramethyl-5-methylene-(13.826%), Phenol, 2,3,4,6-tetramethyl-(11.426%). Con-clusion:Rabdosia amethystoides (Benth.) H. Hara.contains a lot of 2-Methoxy-4-vinylphenol, which is a common food ad-ditives. Rabdosia amethystoides (Benth.) H. Hara.can be comprehensive utilization.

  6. Two myrmecophilous scale insects, Cryptostigma urichi (Cockerell (Hemiptera, Coccidae and Farinococcus multispinosus Morrison (Hemiptera, Pseudococcidae, cohabiting inside branches of Anadenanthera falcata (Benth. Speg. (Fabales, Fabaceae in the Cerrado area of São Paulo State, Brazil Duas cochonilhas mirmecófilas, Cryptostigma urichi (Cockerell (Hemiptera, Coccidae e Farinococcus multispinosus Morrison (Hemiptera, Pseudococcidae, coabitando no interior de ramos de Anadenanthera falcata (Benth. Speg. (Fabales, Fabaceae em área de Cerrado do Estado de São Paulo, Brasil

    Directory of Open Access Journals (Sweden)

    André R. Nascimento

    2012-12-01

    Full Text Available The soft scale Cryptostigma urichi (Cockerell, 1894 and the mealybug Farinococcus multispinosus Morrison, 1922 are reported cohabiting inside branches of Anadenanthera falcata (Benth. Speg. (Angico-preto, both tended by the ant Azteca oecocordia Longino, 2007 (Hymenoptera, Formicidae. This interaction was observed in the Cerrado region sensu stricto, at the Federal University of São Carlos, UFSCar, Brazil. A. oecocordia is recorded for the first time in Brazil.O coccídeo Cryptostigma urichi (Cockerell, 1894 e o pseudococcídeo Farinococcus multispinosus Morrison, 1922 são registrados coabitando no interior de galhos de Anadenanthera falcata (Benth. Speg. (Angico-preto, ambos atendidos pela formiga Azteca oecocordia Longino, 2007 (Hymenoptera, Formicidae. Essa interação foi observada em uma região de Cerrado sensu stricto, na Universidade Federal de São Carlos, UFSCar, Brasil. A. oecocordia é registrada pela primeira vez para o Brasil.

  7. Effects of mutual coupling on the channel capacity of MIMO/SA multi-antenna system%互耦效应对MIMO/SA多天线系统信道容量的影响

    Institute of Scientific and Technical Information of China (English)

    郭爱煌; 肖法; 黄宇胜; 尚秀辉

    2011-01-01

    多输入多输出/智能天线(multiple input multiple output/smart antenna,MIMO/SA)系统是4G的关键技术之一,多天线的互耦效应是影响MIMO/SA多天线系统性能的主要问题.建立了MIMO与SA相结合的多天线系统模型,推导了互耦效应影响下空间相关系数和信道容量的表达式;通过电磁场数值计算和蒙特卡罗方法仿真MIMO/SA多天线系统的各态历经信道容量.结果表明:在典型的角度谱分布下改变天线间距,考虑互耦效应的信道容量围绕无互耦效应时的信道容量上下振荡;基站天线阵列间距增大信道容量持续增大,移动台天线阵列间距增大到0.5倍波长后信道容量基本保持不变;互耦效应增大信道的空域相关性,从而降低信道容量.%Multiple input multiple output and smart antenna (MIMO/SA) system is one of the key technologies for the fourth generation mobile communications system, mutual coupling is the main factor which affects the performance of MIMO/SA multi-antenna systems. A combination model of M1MO and SA is presented. With the existing of mutual coupling effects the expressions of spatial correlation and channel capacity are derived. The ergodic capacity of MIMO/SA multi-antenna systems is simulated using electromagnetic numeric method and Monte Carlo method. The results show that in the typical power azimuth spectrum distribution, the channel capacity which takes mutual coupling into account oscillates up and down around the channel capacity without mutual coupling while changing the distance of the antennas; the channel capacity increases continually while increasing the distance of base station antenna array; when antennas distance of the mobile station is farther than 0. 5 wavelength the channel capacity remains almost the same even though the antenna array distance increases; and mutual coupling increases the channel spatial correlation of MIMO/SA multi-antenna systems and thus reduces the channel capacity.

  8. (Blanco) Benth (Lamiaceae)

    African Journals Online (AJOL)

    analytical gas chromatography (GC) and confirmation of chemical structure ... The effect of PA from Pogostemon cablin on immunological function was studied by ... (DTH) in mice that were administered orally doses of 20, 40 and 80 mg/kg. ... These results suggest that PA has significant immunomodulatory properties which.

  9. Pogostemon cablin (Blanco) Benth

    African Journals Online (AJOL)

    SAM

    2014-06-04

    Jun 4, 2014 ... inhibition for aqueous, ethanol and methanol extracts of patchouli with 20 to 80 µl concentration and no ... The combination of these antimicrobial plant extracts can be used ..... graveolens) with supercritical carbon dioxide.

  10. Influência de hábitos antrópicos na dispersão de Triatoma pseudomaculata Corrêa & Espínola, 1964, através de Mimosa tenuiflora (Willdenow) (Mimosaceae) no Estado do Ceará, Brasil Influence of anthropic habits in the dispersion of Triatoma pseudomaculata Corrêa & Espínola, 1964 through Mimosa tenuiflora (Willdenow) (Mimosaceae) in the State of Ceará, Brazil

    OpenAIRE

    Simone Patrícia Carneiro Freitas; Assilon Lindoval Carneiro Freitas; Severino do Monte Prazeres; Teresa Cristina Monte Gonçalves

    2004-01-01

    Triatoma pseudomaculata foram capturados em entrecascas de Mimosa tenuiflora (jurema-preta) em ecótopo silvestre de sete municípios do Estado do Ceará. Esta planta apresenta alta taxa de cobertura geográfica nas áreas de caatingas, sendo freqüentemente utilizada pela população rural. A presença de triatomíneos no peridomicílio, na região do Cariri, pode estar associada ao uso pela população da madeira de M. tenuiflora, quando trazida do ambiente silvestre, repleta de triatomíneos, facilitando...

  11. UJI ZONA HAMBAT EKSTRAK DAUN PUTRI MALU (Mimosa pudica TERHADAP BAKTERI Staphylococcus aureus DAN Methicillin-resistant Staphylococcus aureus (MRSA SECARA IN VITRO

    Directory of Open Access Journals (Sweden)

    Nyoman Ririn Chandrika Sari

    2015-04-01

    Full Text Available Resistensi Staphylococcus aureus dan MRSA terhadap antibiotika spektrum luas mendorong berbagai penelitian untuk menemukan senyawa aktif yang sensitif dan efektif dalam menghambat pertumbuhan bakteri.Penelitian ini bertujuan untuk mengetahui efek penghambatan ekstrak daun putri malu (Mimosa pudica terhadap pertumbuhan bakteri Staphylococcus aureus dan MRSA.Penelitian terhadap aktivitas antimikrobial ekstrak daun putri malu dilakukan dengan metode agar difusi cakram dengan menggunakan metode Kirby-Bauer.Suspensi bakteri disesuaikan dengan standar kekeruhan Mc Farland 0.5. Biakan bakteri dalam cawan petri masing-masing diberikan 6 perlakuan, yaitu kontrol positif (amoxicillin atau vancomycin, kontrol negatif (alkohol, serta ekstrak daun putri malu dengan konsentrasi 25 mcg/ml, 50 mcg/ml, 75 mcg/ml dan 100 mcg/ml. Pertumbuhan Staphylococcus aureus dihambat secara signifikan oleh ekstrak daun putri malu, dengan efek inhibisi pada semua konsentrasi secara signifikan lebih baik dibandingkan dengan kontrol positif (p<0.05. Zona inhibisi Staphylococcus aureus pada konsentrasi 25 mcg/ml adalah 28.86 mm dan telah memenuhi kriteria sebagai antimikrobial sensitif berdasarkan Clinical and Laboratory Standard Institute (CLSI. Inhibisi pertumbuhan MRSA secara signifikan terjadi pada semua konsentrasi ekstrak daun putri malu dibandingkan dengan kontrol negatif (p=0.00. Zona inhibisi terbesar ditemukan pada konsentrasi 100 mcg/ml dengan diameter sebesar 14.16 mm dan memiliki efek antimikrobial sebanding dengan vancomycin dalam menghambat pertumbuhan MRSA (p=0.186. Hasil uji agar difusi cakram menunjukkan bahwa senyawa aktif dalam ekstrak daun putri malu memiliki aktivitas antimikrobial yang tinggi terhadap Staphylococcus aureus dan MRSA secara in vitro.

  12. In vivo antiprostate tumor potential of Vernonia guineensis Benth. (Asteraceae) tuber extract (VGDE) and the cytotoxicity of its major compound pentaisovaleryl sucrose

    Science.gov (United States)

    Toyang, Ngeh J.; Ateh, Eugene N.; Davis, Harry; Tane, Pierre; Sondengam, Luc B.; Bryant, Joseph; Verpoorte, Rob

    2015-01-01

    Ethnopharmacological relevance Vernonia guineensis Benth. (Asteraceae) root decoction is used in folk medicine in Cameroon to treat some ailments including prostate cancer. The aim of this study was to validate the claimed antiprostate cancer activity of Vernonia guineensis Benth. in vivo and to investigate the cytotoxicity of a pentaisovaleryl sucrose isolated from Vernonia guineensis on some cancer cell lines. Materials and methods A crude dichloromethane extract of Vernonia guineensis (VGDE) was used for this study. For in vivo antiprostate cancer efficacy, nude mice (n = 16) were injected subcutaneously with prostate cancer PC-3 cells. Upon the formation of the xenograft tumors, the mice were divided into two equal groups with approximately the same mean tumor volume per group. One group was treated with VGDE orally (500 mg/kg) and the other with a vehicle control for 30 days. Body weight and tumor volumes were measured 2 × a week and on the 33rd day, the mice were euthanized and tumors harvested and weighed. For the cytotoxicity study, the WST-1 assay was used to determine the activity of pentaisovaleryl sucrose previously isolated from VGDE. The cancer cell lines used in the cytotoxicity study included breast, colon, leukemia, lung, melanoma, ovarian and prostate. Results Prostate cancer (PC-3) xenograft tumors treated with VGDE showed a significant decrease in tumor size (P = 0.0295) compared to control. Pentaisovaleryl sucrose also demonstrated cytotoxicity against various cancer cell lines with IC50 values as follows: MDA-MD-231—6.66 µM; MCF-7—7.50 µM; HCT116—14.12 µM; A549—5.76 µM; HL60—6.43 µM; A375—8.64 µM; OVCAR3—9.53 µM; Capan1—7.13 µM; Mia-Paca 6.47 µM. Conclusion VGDE does possess in vivo activity against prostate tumor and has potential for development into a natural product for the treatment of prostate cancer. This study thus provides preliminary validation for the folk use of Vernonia guineensis against prostate

  13. Seasonal variation of phenolic content in galled and non-galled tissues of Calliandra brevipes Benth (Fabaceae: Mimosoidae Variação sazonal do conteúdo fenólico em tecidos galhados e não-galhados de Calliandra brevipes Benth (Fabaceae: Mimosoidae

    Directory of Open Access Journals (Sweden)

    Michelle de Lima Detoni

    2011-09-01

    Full Text Available Two species, Tanaostigmodes ringueleti and T. mecanga, induce distinct galls on Calliandra brevipes Benth (Fabaceae: Mimosoidae, a globose and a fusiform gall morphotype. Seasonal changes of phenolic content in the tissues of the two distinct galls were compared to those of non-galled leaves and stems of the host plants over one year. The variation in the phenolic content profiles was similar in both non-galled and galled tissues, and was primarily associated with changes in the levels of rainfall, indicating a direct response to hydric stress. In periods of drastic changes in water precipitation, the alterations were significantly higher in non-galled than in galled tissues suggesting that the gall inducers might limit the variation in the phenolic concentration for their own benefit.Duas espécies, Tanaostigmodes ringueleti e T. mecanga, induzem galhas distintas em Calliandra brevipes Benth (Fabaceae: Mimosoidae, um morfotipo globoso e um fusiforme. Mudanças sazonais no conteúdo fenólico nos tecidos das duas galhas foram comparadas àquelas de folha e caule não galhados das plantas hospedeiras por um ano. A variação no perfil de conteúdo fenólico foi similar em tecidos galhados e não galhados, sendo associada primariamente às mudanças nos níveis de chuva, constituindo uma resposta direta ao estresse hídrico. Nos períodos de mudanças drásticas na precipitação de água, as alterações foram significativamente maiores em tecido não galhados do que em tecidos galhados, sugerindo que os galhadores estariam limitando a variação do conteúdo fenólico em seu próprio benefício.

  14. Influência de hábitos antrópicos na dispersão de Triatoma pseudomaculata Corrêa & Espínola, 1964, através de Mimosa tenuiflora (Willdenow (Mimosaceae no Estado do Ceará, Brasil Influence of anthropic habits in the dispersion of Triatoma pseudomaculata Corrêa & Espínola, 1964 through Mimosa tenuiflora (Willdenow (Mimosaceae in the State of Ceará, Brazil

    Directory of Open Access Journals (Sweden)

    Simone Patrícia Carneiro Freitas

    2004-02-01

    Full Text Available Triatoma pseudomaculata foram capturados em entrecascas de Mimosa tenuiflora (jurema-preta em ecótopo silvestre de sete municípios do Estado do Ceará. Esta planta apresenta alta taxa de cobertura geográfica nas áreas de caatingas, sendo freqüentemente utilizada pela população rural. A presença de triatomíneos no peridomicílio, na região do Cariri, pode estar associada ao uso pela população da madeira de M. tenuiflora, quando trazida do ambiente silvestre, repleta de triatomíneos, facilitando conseqüentemente sua mobilização e dispersão.Triatoma pseudomaculata was captured in the phloem of the black acacia bush (Mimosa tenuiflora in seven municipalities in the State of Ceará, Brazil. This bush is widespread in the caatinga (scrub forest and is used extensively by the rural population for kindling. Peridomiciliary presence of triatomines in the Cariri region may be associated with the use of infested M. tenuiflora wood by the population, thus facilitating the triatomines' mobilization and dispersion.

  15. Induction of Osmoregulation and Modulation of Salt Stress in Acacia gerrardii Benth. by Arbuscular Mycorrhizal Fungi and Bacillus subtilis (BERA 71)

    Science.gov (United States)

    Hashem, Abeer; Alqarawi, A. A.; Al-Huqail, A. A.; Shah, M. A.

    2016-01-01

    The role of soil microbiota in plant stress management, though speculated a lot, is still far from being completely understood. We conducted a greenhouse experiment to examine synergistic impact of plant growth promoting rhizobacterium, Bacillus subtilis (BERA 71), and arbuscular mycorrhizal fungi (AMF) (Claroideoglomus etunicatum; Rhizophagus intraradices; and Funneliformis mosseae) to induce acquired systemic resistance in Talh tree (Acacia gerrardii Benth.) against adverse impact of salt stress. Compared to the control, the BERA 71 treatment significantly enhanced root colonization intensity by AMF, in both presence and absence of salt. We also found positive synergistic interaction between B. subtilis and AMF vis-a-vis improvement in the nutritional value in terms of increase in total lipids, phenols, and fiber content. The AMF and BERA 71 inoculated plants showed increased content of osmoprotectants such as glycine, betaine, and proline, though lipid peroxidation was reduced probably as a mechanism of salt tolerance. Furthermore, the application of bioinoculants to Talh tree turned out to be potentially beneficial in ameliorating the deleterious impact of salinity on plant metabolism, probably by modulating the osmoregulatory system (glycine betaine, proline, and phenols) and antioxidant enzymes system (SOD, CAT, POD, GR, APX, DHAR, MDAHR, and GSNOR). PMID:27597969

  16. Study on chemical constituents from bark of Ptychopetalum olacoides Benth%榥榥木树皮化学成分研究

    Institute of Scientific and Technical Information of China (English)

    李军; 唐万侠

    2013-01-01

      利用硅胶柱色谱和HPLC对榥榥木树皮化学成分进行分离,得到3个倍半萜类化合物.经1H NMR,13C NMR等鉴定它们的结构分别为(8,9,10)-8,9-epoxycalamenen-10-ol(1), Eudesm-4(15)-ene-1,6-diol(2),(4S,4aS,6S,8aS)-4a,5,6,7,8,8a-Hexahydro-4-hydroxy-4,8a-dimethyl-6-(1-methylethenyl)naphthalen-1(4H)-one(3).3个化合物均为首次从该植物中分离得到.%Study the chemical constituents from the bark of Ptychopetalum olacoides Benth by column chromatography and HPLC.Three compounds were isolated and their structures were identified as(8,9,10)-8, 9-epoxycalamenen-10-ol(1),Eudesm-4(15)-ene-1,6-diol(2),(4S,4aS,6S,8aS)-4a,5,6,7, 8,8a-Hexahydro-4-hydroxy-4,8a-dimethyl-6-(1-methylethenyl)naphthalen-1(4H)-one(3).The compounds were isolated from this plant for the first time.

  17. Abscisic acid induced changes in production of primary and secondary metabolites, photosynthetic capacity, antioxidant capability, antioxidant enzymes and lipoxygenase inhibitory activity of Orthosiphon stamineus Benth.

    Science.gov (United States)

    Ibrahim, Mohd Hafiz; Jaafar, Hawa Z E

    2013-07-05

    An experiment was conducted to investigate and distinguish the relationships in the production of total phenolics, total flavonoids, soluble sugars, H2O2, O2-, phenylalanine ammonia lyase (PAL) activity, leaf gas exchange, antioxidant activity, antioxidant enzyme activity [ascorbate peroxidase (APX), catalase (CAT), superoxide dismutase (SOD) and Lipoxygenase inhibitory activity (LOX)] under four levels of foliar abscisic acid (ABA) application (0, 2, 4, 6 µM) for 15 weeks in Orthosiphon stamineus Benth. It was found that the production of plant secondary metabolites, soluble sugars, antioxidant activity, PAL activity and LOX inhibitory activity was influenced by foliar application of ABA. As the concentration of ABA was increased from 0 to 6 µM the production of total phenolics, flavonoids, sucrose, H2O2, O2-, PAL activity and LOX inhibitory activity was enhanced. It was also observed that the antioxidant capabilities (DPPH and ORAC) were increased. This was followed by increases in production of antioxidant enzymes APX, CAT and SOD. Under high application rates of ABA the net photosynthesis and stomatal conductance was found to be reduced. The production of primary and secondary metabolites displayed a significant positive relationship with H2O2 (total phenolics, r2 = 0.877; total flavonoids, r2 = 0.812; p ≤ 0.05) and O2- (total phenolics, r2 = 0.778; total flavonoids, r2 = 0.912; p ≤ 0.05). This indicated that increased oxidative stress at high application rates of ABA, improved the production of phytochemicals.

  18. CONTENIDO DE TANINOS EN LA CORTEZA DE DOS ESPECIES DE PARÁCATA (Erythroxylon compactum Rose y Senna skinneri Benth. Irwin & Barneby

    Directory of Open Access Journals (Sweden)

    Serafín Colín-Urieta

    2013-01-01

    Full Text Available Se obtuvieron taninos de la corteza de Parácata (Erythroxylon compactum Rose y Senna skinneri Benth. Irwin & Barneby mediante extracción acuosa aplicando un diseño experimental 2k, con k = 4 y n = 2. Los factores y niveles fueron: Factor A = tamaño de partícula (0.417, 6.68 mm, Factor B = tiempo de extracción (120, 180 min, Factor C = temperatura de extracción (80, 87 °C, Factor D = relación sólido-líquido (1:15, 1:12. Se evaluó la calidad curtiente de los taninos. Se curtió una piel de venado con taninos de S. skinneri y se determinó la resistencia al desagarre y la resistencia a la tensión. Los resultados de las propiedades físico-mecánicas de la piel curtida fueron superiores a los valores mínimos recomendados por las normas mexicanas. Con base en lo anterior, se estima que la corteza de S. skinneri pudiera ser susceptible de aprovechamiento para la extracción de taninos y así usarlos en el curtido de pieles.

  19. ALTERAÇÕES NA ARQUITETURA TÍPICA DE Tipuana tipu (Benth. O. Kuntze NA ARBORIZAÇÃO DE RUAS DE CURITIBA, PARANÁ

    Directory of Open Access Journals (Sweden)

    Rogério Bobrowski

    2013-01-01

    Full Text Available The urban structure and equipments can restrict the correct development of trees and its typical architecture. Tipuana tipu (Benth. O. Kuntze (rosewood has been pruned continuously for being a species of large size and for being the most planted tree in the city of Curitiba. Because of that, this work aimed to assess the changes in the typical architecture of Tipuana tipu on street trees of Curitiba through completely randomized design containing a check plot composed by 6 trees and a treatment with 6 trees under utility lines and another with 6 trees in streets without utility lines. From horizontal photographs of trees there were obtained dendrometric variables based on a metric scale attached at DBH. Thus, the morphometric indexes were calculated to describe dimensional relationships for the species on trees without pruning (check plot and on pruned trees (treatments. The results showed that raising pruning change typical architecture of rosewood modifying its outline and its form, in addition to harm its aesthetic effect. The indexes that can express changes significantly (p<0.05, were: range index, salience index, crown angle, crown proportion and relation RH/PH.

  20. Induction of Osmoregulation and Modulation of Salt Stress in Acacia gerrardii Benth. by Arbuscular Mycorrhizal Fungi and Bacillus subtilis (BERA 71

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    Abeer Hashem

    2016-01-01

    Full Text Available The role of soil microbiota in plant stress management, though speculated a lot, is still far from being completely understood. We conducted a greenhouse experiment to examine synergistic impact of plant growth promoting rhizobacterium, Bacillus subtilis (BERA 71, and arbuscular mycorrhizal fungi (AMF (Claroideoglomus etunicatum; Rhizophagus intraradices; and Funneliformis mosseae to induce acquired systemic resistance in Talh tree (Acacia gerrardii Benth. against adverse impact of salt stress. Compared to the control, the BERA 71 treatment significantly enhanced root colonization intensity by AMF, in both presence and absence of salt. We also found positive synergistic interaction between B. subtilis and AMF vis-a-vis improvement in the nutritional value in terms of increase in total lipids, phenols, and fiber content. The AMF and BERA 71 inoculated plants showed increased content of osmoprotectants such as glycine, betaine, and proline, though lipid peroxidation was reduced probably as a mechanism of salt tolerance. Furthermore, the application of bioinoculants to Talh tree turned out to be potentially beneficial in ameliorating the deleterious impact of salinity on plant metabolism, probably by modulating the osmoregulatory system (glycine betaine, proline, and phenols and antioxidant enzymes system (SOD, CAT, POD, GR, APX, DHAR, MDAHR, and GSNOR.

  1. Anatomia das raízes de Bacopa salzmanii (Benth. Wettst. Ex Edwall e Bacopa Monnierioides (Cham. Robinson (Scrophulariaceae em ambientes aquático e terrestre

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    Bona Cleusa

    2003-01-01

    Full Text Available O presente trabalho enfoca a anatomia das raízes de Bacopa salzmanii (Benth. Wettst. Ex Edwall e B. monnierioides (Cham. Robinson e suas adaptações aos ambientes aquático e terrestre. Foram analisadas raízes adventícias das duas espécies, coletadas no Município de Bonito e no Pantanal do Mato Grosso do Sul. As análises foram feitas do ápice à base da raiz, enfatizando a origem e desenvolvimento dos tecidos. O meristema apical apresenta a mesma estrutura nas duas espécies e não sofre alterações marcantes com a mudança do ambiente. Todos os tecidos se originam de três camadas distintas, na região do promeristema. A endoderme jovem é meristemática e dá origem ao córtex. O aerênquima é abundante e os septos podem conter espessamento em fi. A exoderme é unisseriada e composta por células curtas e longas. A coifa das duas espécies é pouco desenvolvida e apresenta estrutura semelhante nos dois ambientes.

  2. Inhibitory Effect of the Hexane Fraction of the Ethanolic Extract of the Fruits of Pterodon pubescens Benth in Acute and Chronic Inflammation

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    Jaqueline Hoscheid

    2013-01-01

    Full Text Available Fruits of Pterodon pubescens Benth have been used traditionally for the treatment of rheumatism, sore throat, and respiratory disorders, and also as anti-inflammatory, analgesic, depurative, tonic, and hypoglycemic agent. The study was aimed at evaluating the anti-inflammatory activity of the hexane fraction of an ethanolic extract of P. pubescens fruits. The oil from P. pubescens fruits was extracted with ethanol and partitioned with hexane. The anti-inflammatory activity was measured with increasing doses of the hexane fraction (FHPp by using a carrageenan-induced rat model of pleurisy and a rat model of complete Freund's adjuvant-induced arthritis by using an FHPp dose of 250 mg/kg for 21 days. Treatment with an FHPp resulted in anti-inflammatory activity in both models. The results of biochemical, hematological, and histological analyses indicated a significant decrease in glucose, cholesterol, and triglycerides levels (18.32%, 34.20%, and 41.70%, resp. and reduction in the numbers of total leukocytes and mononuclear cells. The FHPp dose of 1000 mg/kg induced no changes in behavioral parameters, and no animal died. The results of this study extend the findings of previous reports that have shown that administration of extracts and fractions obtained from species of the genus Pterodon exhibits anti-inflammatory activity and lacks toxicity.

  3. First report of toxicity of Xylopiaparviflora (A. Rich.) Benth (Annonaceae) root bark's essential oil against cowpea seed bruchid, Callososbruchus maculatus Fabricius (Coleoptera: Chrysomelidae: Bruchinae).

    Science.gov (United States)

    Babarinde, Samuel Adelani; Pitan, Olufemi Olutoyin Richard; Olatunde, Ganiyu Olatunji; Ajala, Michael Oluwole

    2015-01-01

    The fumigant toxicity of Xylopia parviflora (A. Rich.) Benth (Annonaceae) root bark's essential oil (EO) against cowpea seed bruchid, Callosobruchus maculatus, was investigated in the laboratory. Dose had significant (P < 0.0001) effect on mortality at 6 hours after treatment (HAT) at a concentration of 6.25 μL/mL air which exerted 81.70% mortality, while there was no mortality in all other lower doses. At 12 HAT, 75.05% and 90.00% mortality were observed at doses of 3.15 and 6.25 μL/mL air, respectively. It was significantly (P < 0.05) higher than the mortality (50.58%) observed when 0.78 μL/mL air was applied. The lethal time for 50% of assayed adults (LT50) obtained when the bruchid was exposed to X. parviflora EO at a dose of 6.25 μL/mL air (2.71 h) was significantly lower than LT50 obtained at exposure of bruchid to other lower doses of 0.78-3.15 μL/mL air.

  4. Influence of Npk inorganic fertilizer treatment on the proximate composition of the leaves of Ocimum gratissimum (L.) and Gongronema latifolium (benth).

    Science.gov (United States)

    Osuagwu, G G E; Edeoga, H O

    2013-04-15

    The influence of NPK inorganic fertilizer treatment on the proximate composition of the leaves of Ocimum gratissimum (L.) and Gongronema latifolium (Benth) was investigated. Cultivated O. gratissimum and G. latifolium were treated with NPK (15:15:15) fertilizer at 100, 200, 300, 400 and 500 kg h(-1) treatment levels in planting buckets derived using the furrow slice method two months after seedling emergence. No fertilizer treatment served as control. The leaves of the plants were harvested for analysis one month after treatment. The leaf was used for the analysis because it the most eaten part. Fertilizer treatment significantly (p fertilizer treatment significantly, (p fertilizer of fertilizer treatment might be due to the role of fertilizer in chlorophyll content of plant's leaves, which in turn enhanced the process of photosynthesis leading to increased synthesis of these substances. The decrease in the carbohydrate content might be due to its conversion to other materials in the plants. The results obtained were discussed in line with current literatures.

  5. Caracterización de la resistencia a la antracnosis causada por Glomerella cingulata y productividad de cinco genotipos de mora (Rubus glaucus Benth.

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    Juan Manuel López-Vásquez

    2013-04-01

    Full Text Available En la vereda Betania, municipio de Guática, Risaralda (Colombia a 2160 m.s.n.m., se evaluaron cinco materiales promisorios de mora (Rubus glaucus Benth. por su resistencia a la antracnosis (Colletotrichum gloeosporioides y por su alta productividad, los cuales fueron previamente seleccionados por el grupo de Investigación en Biodiversidad y Biotecnología de la Universidad Tecnológica de Pereira. Diez meses después del establecimiento las plantas fueron inoculadas con diferentes cepas del hongo a una concentración de 1.2 x 10(6 conidios/ml de agua. Las variables evaluadas fueron incidencia (presencia o ausencia de lesiones en tallo, severidad (según escala de 1 a 9 y rendimiento (kg/planta. Los genotipos se clasificaron en tres grupos según su resistencia al patógeno y por su rendimiento: el primero, lo conformaron UTP-8 y UTP-7, los cuales produjeron 21 y 17.6 t/ha, respectivamente; el segundo grupo estuvo formado por el genotipo UTP-1 el cual produjo 14.3 t/ha, y el tercero por UTP-2 y UTP-4 con 9.8 y 7.9 t/ha, respectivamente.

  6. Malformações, abortos e mortalidade embrionária em ovinos causada pela ingestão de Mimosa tenuiflora (Leguminosae

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    José Rômulo Soares dos Santos

    2012-11-01

    Full Text Available Para determinar a teratogenicidade de Mimosa tenuiflora, 15 ovelhas, distribuídas em dois grupos, foram introduzidos em área invadida pela planta. O Grupo 1, com seis ovelhas prenhes, foi introduzido na área experimental 20 dias após o acasalamento. O Grupo 2, formado por nove ovelhas não prenhes e um carneiro, foi introduzido na área experimental no início do experimento. O experimento começou no mês de outubro de 2009, período de estiagem, quando M. tenuiflora estava sementando. Nesse período as plantas foram rebaixadas a 40 cm de altura e os galhos com folhas e sementes foram disponibilizados para os ovinos na mesma área onde M. tenuiflora foi rebaixada. M. tenuiflora começou a rebrotar ainda na estação seca antes do período de chuvas. No período de rebrota, as ovelhas ficavam livres para pastar M. tenuiflora e recebiam concentrado em quantidade equivalente a 1% do peso vivo. Após as primeiras chuvas, em meados de janeiro do ano seguinte, quando o estrato herbáceo apareceu, essas ovelhas foram confinadas em baias, onde M. tenuiflora foi fornecida até o fim do experimento. A cada 15 dias eram realizados exames ultrassonográficos para acompanhamento da gestação. No Grupo 1, três ovelhas abortaram, cada uma um feto sem malformações. Outra ovelha pariu dois cordeiros, um com hiperflexão na articulação inter-falangeana proximal no membro torácico direito e outro sem malformações. Outra ovelha pariu um cordeiro com hiperflexão dos dois membros pélvicos na região da articulação tarso-metatársica. No grupo formado pelas ovelhas que foram acasaladas na área experimental, uma ovelha abortou um feto sem malformações e cinco pariram cordeiros normais. Três das ovelhas desse grupo não emprenharam durante todo o período experimental, mostrando retornos repetidos ao cio, sugerindo perda embrionária. Trinta e duas ovelhas e um carneiro, que permaneceram numa área vizinha a área experimental e foram utilizados

  7. Investigação dos efeitos citotóxico, genotóxico e antibacteriano dos taninos isolados de Anacardium Occidentale Linn e Anadenanthera Macrocarpa (Benth.) Brenan em linhagens de Streptococcus associados ao biofilme dental

    OpenAIRE

    Araújo, Josenildo Segundo Chaves de

    2013-01-01

    As plantas são uma rica fonte de novas moléculas biologicamente ativas com reconhecido potencial no desenvolvimento de medicamentos. O Anacardium occidentale Linn é popularmente conhecida como 'cajueiro', é uma planta da família das Anacardiaceae, originária do Nordeste do Brasil e popularmente conhecida por suas propriedades antiinflamatórias e cicatrizantes. O Anadenanthera macrocarpa (Benth.) Brenan ou o "angico" é pertencente a família das Mimosaceae, distribuída pelas r...

  8. Conservación de mora de castilla (rubus glaucus benth) mediante la aplicación de un recubrimiento comestible de gel de mucílago de penca de sábila (aloe barbadensis miller)

    OpenAIRE

    Ramírez Quirama, Jhon David

    2012-01-01

    La mora de Castilla (Rubus glaucus Benth) es una fruta comercialmente importante, en la venta al detalle y en la industrial, pese a que es un producto altamente perecedero. Los recubrimientos comestibles (RC) han tenido mucho auge como método de conservación sobre las frutas a nivel mundial debido a su fácil implementación y costo relativamente bajo. El aloe vera es una alternativa bastante importante en los RC, debido a sus características mucílaginosas y a su poder antimicrobiano, a demás q...

  9. Acute and sub-chronic toxicity studies of three plants used in Cameroonian ethnoveterinary medicine: Aloe vera (L.) Burm. f. (Xanthorrhoeaceae) leaves, Carica papaya L. (Caricaceae) seeds or leaves, and Mimosa pudica L. (Fabaceae) leaves in Kabir chicks.

    Science.gov (United States)

    Nghonjuyi, Ndaleh Wozerou; Tiambo, Christian Keambou; Taïwe, Germain Sotoing; Toukala, Jean Paul; Lisita, Frederico; Juliano, Raquel Soares; Kimbi, Helen Kuokuo

    2016-02-03

    Aloe vera (L.) Burm. f. (Xanthorrhoeaceae), Carica papaya L. (Caricaceae) and Mimosa pudica L. (Fabaceae) are widely used in the Cameroonian ethnoveterinary medicine as a panacea, and specifically for gastrointestinal disorders as well as an anthelmintic and antibacterial. The present study evaluated the potential toxicity of the hydroalcoholic extracts of Aloe vera leaves, Carica papaya leaves or seeds, and Mimosa pudica leaves after acute and sub-chronic administration in chicks. For the acute toxicity test a single administration of each of the four hydroalcoholic extracts was given orally at doses ranging from 40 to 5120 mg/kg (n=5/group/sex). In the sub-chronic study, these extracts were given orally as a single administration to chicks at doses of 80, 160, 320 and 640 mg/kg/day for 42 days. The anti-angiogenic properties of these extracts (5-320 µg/mg) were investigated in the chick chorioallantoic membrane in vivo. In the acute toxicity test, none of the four studied hydroalcoholic extracts induced mortality or significant behavioural changes. The sub-acute treatment with the four plant extracts did not alter either the body weight gain or the food and water consumption. However, the results indicated that Aloe vera leaf extract acute treatment by oral route at doses up to 2560 mg/kg did not produce death in 50% (5/10) of chicks during 24h or 14 days of observation, but 20% (2/10) chicks died. The haematological and biochemical analyses did not show significant differences in any of the parameters examined in female or male groups, with the exception of a transient rise in white blood cell counts at high doses (640 mg/kg). Additionally, these extracts did not have the potential for anti-angiogenic effects through the inhibition of neo-angiogenesis in the chick chorioallantoic membrane in vivo. The results showed that the therapeutic use of the hydroalcoholic extracts of Aloe vera leaves, Carica papaya leaves or seeds and Mimosa pudica leaves had very low

  10. Germinación, dispersión y establecimiento de plántulas de Mimosa tenuiflora (Leguminosae en México

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    Sara L. Camargo-Ricalde

    1998-09-01

    Full Text Available Mimosa tenuiflora (Willd. Poir. es un recurso natural importante para México debido a sus diversos usos. Sin embargo, hasta ahora no se había estudiado la germinación, la dispersión y el establecimiento de plántulas de esta especie. Se recolectaron frutos y semillas de esta planta en el estado de Chiapas, México (S. L. Camargo et al. 118, UAMIZ. Las pruebas de germinación se hicieron después de cuatro años de almacenamiento; se sembraron en total 1 935 semillas en cajas de Petri con papel filtro Wathman humedecido. Se determinaron las temperaturas óptimas usando un gradiente de 5-40°C, con luz continua, para semillas escarificadas mecánicamente y no escarificadas. Se realizaron tres pruebas de fotoperíodo: a luz continua, b oscuridad constante y c luz 12hr/oscuridad 12 hr. Se aplicaron tres tratamientos pregerminativos: a escarificación mecánica, b ácido sulfúrico concentrado, c fuego directo y d ningún tratamiento. Para cada variable y pretratamiento se hicieron tres repeticiones, cada una con 15 semillas. Se aplicaron las pruebas estadísticas correspondientes: desviación estandar (S, análisis de varianza de una vía (ANOVA y comparación de medias (Prueba de Scheffé (pMimosa tenuiflora (Willd. Poir. is an important natural resource in Mexico, due to its diverse uses. However, seed germination, dissemination and seedling establishment of this species had not been studied up to date. Fruits and seeds were collected in the State of Chiapas, Mexico (S. L. Camargo et al. 118, UAMIZ. Germination experiments were carried out after four years of seed storage; 1 935 seeds were sown in Petri dishes containing moistened Wathman filter paper. Optimum temperatures were determined in a gradient 5-40 0C, with continuous light for mechanically scarified and non scarified seeds. Three photoperiod experiments were made: a continuous light, b constant darkness and c 12 hr light/12 hr darkness. Three germinate pretreatments were applied: a

  11. Drift of sub-doses of glyphosate in young plants of Varjão (Parkia multijuga, Benth. Deriva de sub-dosis de glyphosate en plantas jóvenes de Varjão (Parkia multijuga Benth. Deriva de sub-doses de glyphosate em plantas jovens de Varjão (Parkia multijuga Benth.

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    Oscar Mitsuo Yamashita

    2011-09-01

    Full Text Available

    Este estudio tuvo como objetivo evaluar la respuesta a la toxicidad de diferentes sub-dosis de glyphosate en las plantas jóvenes de Varjão (Parkia multijuga Benth.. El experimento se ha instalado en área experimental de la UNEMAT - Universidad de lo Estado de Mato Grosso, en el Campus de Alta Floresta, Alta Floresta-MT. Los tratamientos fueron tres sub-dosis de glifosato (T1-6%, T2-12%, T3-24% de la dosis recomendada de 3,0 L ha-1 y T4 - control (sin aplicación. Después de 7, 14, 21, y 28 días y de 6 meses de aplicación del herbicida, se hicieron mediciones de la altura y de lo diámetro de las plantas con la atribución de las notas de fitotoxicidad. Después de 6 meses de aplicación del herbicida, se encontró que dosis crecientes de glyphosate provocan lesiones en las hojas y reducción del crecimiento en altura de las plantas de Parkia multijuga, hubo uno pequeño índice de mortalidad, sin embargo, se observó una mejoría significativa en todos los tratamientos probados.

    This work aimed to evaluate the response to toxicity of different sub-doses of glyphosate in young plants of varjão (Parkia multijuga, Benth.. The experiment was installed in the experimental area of UNEMAT – State University of Mato Grosso, in Alta Floresta – Mato Grosso state. The treatments tested were three sublethal rates of glyphosate (T1-6%, T2-12%, T3-24% of the recommended dose of 3.0 L ha-1 and T4-control (without application. After 7, 14, 21, 28 days and 6 months of the application of the herbicide, it was performed measurements of the height and diameter of the cervix of plants and given the notes of phytotoxicity. After 6 months of the application of the herbicide, it was found that the increasing doses of glyphosate caused injuries in the leaves and reduction of growth in height in plants of Parkia multijuga, there was low index of mortality

  12. Diâmetro do caule sobre a desidratação, composição química e produção do feno de Jurema preta (Mimosa tenuiflora Wild. Poir. Diameter on branch dehydration, chemical composition and hay production in Jurema preta (Mimosa tenuiflora Wild. Poir.

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    José Rômulo Soares dos Santos

    2011-03-01

    Full Text Available Objetivou-se avaliar o efeito do diâmetro do caule na desidratação, composição química e disponibilidade de feno da Jurema preta (Mimosa tenuiflora. As plantas estavam com média de 3 metros de altura e em final de floração. Foram selecionados caules representativos nos diâmetros 5, 6, 7, 8 e 9mm, os quais foram cortados, separados em caule e folhas e submetidos ao processo de desidratação que consistiu na secagem em estufa de circulação de ar forçada a uma temperatura de 65°C até peso constante. A curva de desidratação foi obtida em função dos intervalos de tempo de pesagem. Para as demais variáveis utilizou-se o delineamento inteiramente casualizado, com cinco tratamentos e cinco repetições. A desidratação de caule e folhas teve resposta linear com platô, maior para diâmetros de 5mm cuja perda foi de 0,02g de água/g matéria seca ao final de 15,84 horas no caule, e de 0,01g de água/g MS na folha a partir 13,48 horas. Os teores de proteína bruta do caule diminuíram e os de fibra em detergente neutro e fibra em detergente ácido aumentaram com o diâmetro do caule, mas não houve influência na composição química da folha. Se o objetivo for qualidade, os caules de Jurema preta devem ser cortados com diâmetros inferiores a 6mm; se o objetivo for quantidade o diâmetro deve ser de 7 a 9mm; se a finalidade for adequar qualidade e quantidade, recomenda-se cortar os caules ao atingirem 6mm de diâmetro.The objective of this work was to evaluate the effect of stem diameter in branch dehydration, chemical composition and hay production in Jurema preta (Mimosa tenuiflora. The selected plants were about three feet high and were in the end of blooming. Representative branches were selected with 5, 6, 7, 8 and 9 mm diameters, which were sliced and separated into stems and leaves and submitted to the dehydration process. It consisted of drying in the forced air circulation stove at a 65°C temperature until the constant

  13. Abscisic Acid Induced Changes in Production of Primary and Secondary Metabolites, Photosynthetic Capacity, Antioxidant Capability, Antioxidant Enzymes and Lipoxygenase Inhibitory Activity of Orthosiphon stamineus Benth.

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    Mohd Hafiz Ibrahim

    2013-07-01

    Full Text Available An experiment was conducted to investigate and distinguish the relationships in the production of total phenolics, total flavonoids, soluble sugars, H2O2, O2−, phenylalanine ammonia lyase (PAL activity, leaf gas exchange, antioxidant activity, antioxidant enzyme activity [ascorbate peroxidase (APX, catalase (CAT, superoxide dismutase (SOD and Lipoxygenase inhibitory activity (LOX] under four levels of foliar abscisic acid (ABA application (0, 2, 4, 6 µM for 15 weeks in Orthosiphon stamineus Benth. It was found that the production of plant secondary metabolites, soluble sugars, antioxidant activity, PAL activity and LOX inhibitory activity was influenced by foliar application of ABA. As the concentration of ABA was increased from 0 to 6 µM the production of total phenolics, flavonoids, sucrose, H2O2, O2−, PAL activity and LOX inhibitory activity was enhanced. It was also observed that the antioxidant capabilities (DPPH and ORAC were increased. This was followed by increases in production of antioxidant enzymes APX, CAT and SOD. Under high application rates of ABA the net photosynthesis and stomatal conductance was found to be reduced. The production of primary and secondary metabolites displayed a significant positive relationship with H2O2 (total phenolics, r2 = 0.877; total flavonoids, r2 = 0.812; p ≤ 0.05 and O2− (total phenolics, r2 = 0.778; total flavonoids, r2 = 0.912; p ≤ 0.05. This indicated that increased oxidative stress at high application rates of ABA, improved the production of phytochemicals.

  14. Perennial peanut (Arachis glabrata Benth.) leaves contain hydroxycinnamoyl-CoA:tartaric acid hydroxycinnamoyl transferase activity and accumulate hydroxycinnamoyl-tartaric acid esters.

    Science.gov (United States)

    Sullivan, Michael L

    2014-05-01

    Many plants accumulate hydroxycinnamoyl esters to protect against abiotic and biotic stresses. Caffeoyl esters in particular can be substrates for endogenous polyphenol oxidases (PPOs). Recently, we showed that perennial peanut (Arachis glabrata Benth.) leaves contain PPO and identified one PPO substrate, caftaric acid (trans-caffeoyl-tartaric acid). Additional compounds were believed to be cis- and trans-p-coumaroyl tartaric acid and cis- and trans-feruloyl-tartaric acid, but lack of standards prevented definitive identifications. Here we characterize enzymatic activities in peanut leaves to understand how caftaric acid and related hydroxycinnamoyl esters are made in this species. We show that peanut leaves contain a hydroxycinnamoyl-CoA:tartaric acid hydroxycinnamoyl transferase (HTT) activity capable of transferring p-coumaroyl, caffeoyl, and feruloyl moieties from CoA to tartaric acid (specific activities of 11 ± 2.8, 8 ± 1.8, 4 ± 0.8 pkat mg(-1) crude protein, respectively). The HTT activity was used to make cis- and trans-p-coumaroyl- and -feruloyl-tartaric acid in vitro. These products allowed definitive identification of the corresponding cis- and trans-hydroxycinnamoyl esters extracted from leaves. We tentatively identified sinapoyl-tartaric acid as another major phenolic compound in peanut leaves that likely participates in secondary reactions with PPO-generated quinones. These results suggest hydroxycinnamoyl-tartaric acid esters are made by an acyltransferase, possibly a BAHD family member, in perennial peanut. Identification of a gene encoding HTT and further characterization of the enzyme will aid in identifying determinants of donor and acceptor substrate specificity for this important class of biosynthetic enzymes. An HTT gene could also provide a means by genetic engineering for producing caffeoyl- and other hydroxycinnamoyl-tartaric acid esters in forage crops that lack them.

  15. Micorrizas arbusculares asociadas al pasto angleton (Dichathium aristatum Benth en fincas ganaderas del municipio de Tolú, Sucre-Colombia

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    Alexander Pérez C.

    2013-03-01

    Full Text Available Objetivo. El presente estudio consistió en caracterizar el porcentaje de colonización de hongos formadores de micorrizas arbusculares (HMA; asociados a la rizósfera del pasto Angleton (Dichanthium aristatum Benth de fincas ganaderas del municipio de Tolú, departamento de Sucre, Colombia. Materiales y métodos. Se muestrearon en total 35 fincas ganaderas, de las cuales se tomaron 10 muestras de suelo con raíces por finca a una profundidad de 20 cm. De cada muestra se aislaron esporas de HMA, se hizo separación de morfotipos y su respectiva identificación a través de claves taxonómicas. De las raíces colectadas se determinó el porcentaje de colonización utilizando métodos de coloración. Resultados. Los ANAVA multifactorial entre las variables densidad de esporas/100 g de suelo y porcentaje de colonización en función a zonas y corregimiento mostraron diferencias altamente significativas. Los resultados obtenidos indican que el 92% de las especies encontradas corresponden a características de hongos HMA similares al género Glomus, el 4% al género Gigaspora y el 4% restante a Paraglomus. Conclusiones. Este estudio preliminar realizado en el Caribe colombiano muestra la diversidad, densidad poblacional y el porcentaje de colonización de HMA asociadas al pasto Angleton en condiciones de siete corregimientos pertenecientes a dos zonas agrológicas del municipio de Tolú, departamento de Sucre.

  16. Micropropagation of Pithecellobium dulce (Roxb.) Benth-a multipurpose leguminous tree and assessment of genetic fidelity of micropropagated plants using molecular markers.

    Science.gov (United States)

    Goyal, Pooja; Kachhwaha, Sumita; Kothari, S L

    2012-04-01

    An efficient and reproducible protocol has been developed for in vitro propagation of Pithecellobium dulce (Roxb.) Benth (a multipurpose leguminous tree) from field grown nodal segments (axillary bud). Shoot bud induction occurred from nodal explants of 15-years-old tree on Murashige and Skoog (MS) basal medium supplemented with 4.4 μM 6-benzyladenine (BA) and multiplication was achieved on MS medium supplemented with 4.4 μM BA + 0.73 μM phenylacetic acid (PAA) i.e. up to 7 shoot buds in the period of 5-6 weeks. Addition of adenine sulphate (AdS) to this medium further enhanced the number of shoot buds up to 10. Proliferating shoot cultures were established by repeatedly subculturing primary culture on fresh medium (MS + 4.4 μM BA + 0.73 μM PAA) after every 25 days. In vitro rooting was achieved on MS medium supplemented with 2.46 μM Indole-3-butyric acid (IBA) + 41.63 μM activated charcoal (AC). The micropropagated shoots with well developed roots were acclimatized in green house in pots containing sand, soil and manure (1:1:1). Genetic stability of micropropagated clones was evaluated using Random amplified polymorphic DNA (RAPD) and Inter simple sequence repeat (ISSR) markers. The amplification products were monomorphic in micropropagated plants and similar to those of mother plant. No polymorphism was detected revealing the genetic uniformity of micropropagated plants. This is the first report of an efficient protocol for regeneration of P. dulce through organogenesis, which can be used for further genetic transformation and pharmaceutical purposes.

  17. Anti-nociceptive Activity of Ethnomedicinally Important Analgesic Plant Isodon rugosus Wall. ex Benth: Mechanistic Study and Identifications of Bioactive Compounds.

    Science.gov (United States)

    Zeb, Anwar; Ahmad, Sajjad; Ullah, Farhat; Ayaz, Muhammad; Sadiq, Abdul

    2016-01-01

    Isodon rugosus Wall. ex Benth. is extensively used as traditional medicine for the management of various types of pain including tooth ache, gastric pain, abdominal pain, ear ache, and generalized body pain. The current study is designed to scientifically verify the purported uses of I. rugosus as analgesic agent and to figure out its possible mechanism of action. Bioactive compounds responsible for analgesic activity were identified using GC and GC-MS analysis. Analgesic potentials were evaluated using acetic acid induced writhing, hot plate test, and formalin induced paw licking test. In acetic acid induced writhing chloroform fraction (Ir.Chf) exhibited 53% analgesia while formalin test displayed 61% inhibition at phase-I and 45% at phase-II respectively at a dose of 100 mg/kg. Similarly, in hot plate test Ir.Chf displayed average reaction time of 7 min at 15, 30, 45, and 60 min intervals. The possible mechanism of action was found to be the central pathway via opioidergic receptors as the mice showed morphine like analgesic activity at pre-administration of naloxone (opioid antagonist) in hot plate and formalin tests. In GC-MS analysis, 83 compounds were identified among which eight compounds including benzyl alcohol, sebacic acid, myristic acid, phytol, sugiol, Tocopherol, α-Amyrin, and stigmasterol were sorted out as previously reported analgesic compounds. Current study revealed that analgesic potential of I. rugosus can attributed to the presence of analgesic compounds. It may also be concluded that opioids receptors are involved in the analgesic mechanism of I. rugosus due to effective antagonism of nalaxone.

  18. A phenylalanine ammonia-lyase ortholog (PkPAL1) from Picrorhiza kurrooa Royle ex. Benth: molecular cloning, promoter analysis and response to biotic and abiotic elicitors.

    Science.gov (United States)

    Bhat, Wajid Waheed; Razdan, Sumeer; Rana, Satiander; Dhar, Niha; Wani, Tariq Ahmad; Qazi, Parvaiz; Vishwakarma, Ram; Lattoo, Surrinder K

    2014-09-01

    Picrorhiza kurrooa Royle ex Benth. is a highly reputed medicinal herb utilised in the preparation of a number of herbal drug formulations, principally due to the presence of novel monoterpene iridoid glycosides kenned as picrosides. Phenylalanine ammonia-lyase catalyses an important rate-limiting step in phenylpropanoid pathway and supplies precursors like cinnamic acid, vanillic acid, ferulic acid, etc., to a variety of secondary metabolites including picrosides. The imperilled status of P. kurrooa coupled with lack of information regarding biogenesis of picrosides necessitates deciphering the biosynthetic pathway for picrosides. In the present study, a PAL gene, designated PkPAL1 was isolated from P. kurrooa. The cDNA is 2312 bp in length, consisting of an ORF of 2142 bp encoding for a 713 amino acid protein having a predicted molecular weight of 77.66 kDa and an isoelectric point of pH 6.82. qRT-PCR analysis of various tissues of P. kurrooa showed that PkPAL1 transcript levels were highest in the leaves, consistent with picroside accumulation pattern. Using Genome walking, a 718 bp promoter region was also isolated resulting in identification of distinct cis-regulatory elements including TGA-element, TGACG-motif, CGTCA-motif, etc. qRT-PCR indicated up-regulation of PkPAL1 by methyl jasmonate, salicylic acid, 2,4-dicholorophenoxy acetic acid and UV-B elicitations that corroborated positively with the identified cis-elements within the promoter region. Moreover, altitude was found to have a positive effect on the PkPAL1 transcript levels, driving the expression of PkPAL1 abundantly. Based on docking analysis, we identified eight residues as potentially essential for substrate binding in PkPAL1.

  19. Controles físico, físico-químico, químico e microbiológico dos frutos de Dimorphandra mollis Benth., Fabaceae Physic, physic-chemical, chemical and microbiological control of fruits of Dimorphandra mollis Benth., Fabaceae

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    Silviane Z. Hubinger

    2009-09-01

    Full Text Available Considerando-se a qualidade dos fitoterápicos, é importante salientar que a preocupação com esta questão inclui rigoroso acompanhamento das diferentes etapas de desenvolvimento e produção, desde a coleta do vegetal até o produto final. O controle de qualidade de drogas vegetais e seus extratos é essencial quando utilizados como matéria-prima para o desenvolvimento de fitoterápicos. Neste trabalho foram utilizadas diversas técnicas (farmacopeicas e não farmacopeicas visando estabelecer parâmetros de qualidade dos frutos de Dimorphandra mollis Benth. (Leguminosae, popularmente conhecida como faveiro. Os resultados obtidos determinaram as características físico-químicas da droga vegetal e mostraram que o pó dos frutos e o extrato etanólico 70% de D. mollis apresentam um teor de flavonóides de 10,25% e 17,21%, respectivamente. A análise fitoquímica preliminar dos frutos caracterizou a presença de taninos, saponinas e flavonóides. Na análise microbiológica do extrato não houve crescimento de patógenos entre os testes realizados.Considering the quality of phytotherapic agents, it is important to point out that the concern with this subject includes rigorous attendance the different steps of development and production of these products, from the collection of the vegetable to the availability of the final product. The quality control for the plants and their extracts is essential when they are used in the development of phytotherapics. In this work, methodologies (pharmacopoeic and no pharmacopoeic were employed to establish norms for the quality control of Dimorphandra mollis Benth. (Leguminosae fruit, popularly known as faveira. Pharmacopoeic and no pharmacopoeic methodologies were employed to physic, physic-chemical, chemical and microbiological quality control. The results obtained established the physic-chemical characters of the drug and showed that the fruits and the ethanol 70% extract present a content of flavonoids

  20. Mobilização de reservas durante a germinação das sementes e crescimento das plântulas de Caesalpinia peltophoroides Benth. (Leguminosae-Caesalpinoideae Mobilization of the reserves during germination of seeds and growth of seedlings of Caesalpinia peltophoroides Benth. (Leguminosae-Caesalpinoideae

    Directory of Open Access Journals (Sweden)

    Viviana Borges Corte

    2006-12-01

    Full Text Available Este trabalho teve como objetivo estudar a mobilização de reservas de sementes de Caesalpinia peltophoroides Benth. durante a germinação e crescimento inicial das plântulas. As variações nas reservas de carbiodratos, lipídios e proteínas foram analisadas desde o período pré-germinativo (0 a 5 dias após a semeadura - DAS até a total senescência e abscisão dos cotilédones, aos 35 DAS, por meio de testes bioquímicos nos cotilédones das sementes. Os resultados indicaram que os lipídios constituem o principal composto de reserva nos cotilédones, contribuindo com cerca de 50% de massa seca. Carboidratos solúveis representaram 32%, as proteínas solúveis 7,7% e o amido 6,8% de massa seca dos cotilédones. Os lipídios sofreram marcante decréscimo entre 5 e 10 dias após a semeadura, período em que se observou elevada taxa de crescimento das plântulas. Carboidratos e proteínas solúveis exibiram tendência gradativa de queda, enquanto no amido, isso quase não foi detectado. A redução do peso de massa seca dos cotilédones foi bem correlacionada com o aumento da biomassa da plântula.This work aimed at studying the mobilization seed reserves during germination and initial growth of seedlings of Caesalpinia peltophoroides Benth. The variations in carbohydrate, lipid and protein reserves were analyzed from the pre-sprouting period (0 to 5 days after sowing -DAS to the total senescence and abscission of seeds. The results showed that lipids constitute the main reserve compound in the cotyledon, contributing with almost 50 % of its dry mass weight. Soluble carbohydrates represent 32 %, the soluble proteins 7.7 % and starch 6.8 % of the dry mass weight of cotyledons. Lipids showed a marked decrease between 5 and 10 days after sowing, period of a high seedling growth rate. Carbohydrates and soluble proteins showed a gradual tendency to decrease, while starch was almost non-detectable. The reduction in cotyledon dry mass of weight

  1. Validated high-performance liquid chromatographic method for the standardisation of Ptychopetalum olacoides Benth., Olacaceae, commercial extracts Validação de método de cromatografia líquida de alta performance para padronização de extratos comerciais de Ptychopetalum olacoides Benth., Olacaceae

    Directory of Open Access Journals (Sweden)

    Renata Colombo

    2010-11-01

    Full Text Available Ptychopetalum olacoides Benth., Olacaceae, popularly known as marapuama or muirapuama or miriantã, is a species native to the Amazonian region of Brazil. Extracts of the bark of the plant have been used traditionally for its stimulating and aphrodisiac properties and currently commercialised by the herbal industry as constituents in a wide range of phytomedicines. Fractionation by open column chromatography followed by preparative HPLC-UV/PAD of the stem bark and of three commercial extracts of P. olacoides allowed the isolation of three components that were common to all extracts analysed, and these were identified by NMR to be vanillic acid, protocatechuic acid and theobromine. Vanillic acid, which has been proposed as a phytochemical marker for P. olacoides, was employed as an external standard in the development and validation of a rapid qualitative and quantitative HPLC assay for the analyte. The recoveries values of the developed method were 99.02% and the LOD and LOQ values were 0.033 and 0.11 mg.L-1, respectively. The described method may be applied to the standardisation of herbs, extracts or phytomedicines commercialised as marapuama.Ptychopetalum olacoides Benth., Olacaceae, popularmente conhecida como marapuama, muirapuama ou miriantã, é uma espécie nativa da região da Amazônia do Brasil. Extratos das cascas da planta são tradicionalmente usados por suas propriedades estimulantes e afrodisíacas, e frequentemente comercializados como constituinte de uma grande variedade de formulações fitoterápicas. O fracionamento por coluna cromatográfica aberta seguida por CLAE-UV/PAD das cascas do caule de três extratos comerciais de P. olacoides permitiram o isolamento de três substâncias comuns em todos os extratos analisados. Os compostos foram identificados por RMN como ácido vanílico, ácido protocatecuíco e teobromina. O ácido vanílico foi utilizado como marcador fitoquímico para P. olacoides e empregado como padr

  2. Modelagem da biomassa total e da lenha por unidade de área para bracatingais nativos

    Directory of Open Access Journals (Sweden)

    Ronan Felipe de Souza

    2014-02-01

    Full Text Available A bracatinga (Mimosa scabrella Benth. é uma espécie pioneira nativa da Floresta Ombrófila Mista de rápido crescimento e cultivada, predominantemente, na forma de povoamentos puros ou consorciada com culturas agrícolas pelos pequenos e médios produtores da região metropolitana de Curitiba. O objetivo deste estudo foi selecionar equações estimativas da biomassa total e da lenha de bracatingais por unidade de área nas diferentes idades, disponibilizando, assim, uma ferramenta simples e de fácil aplicação durante o processo de comercialização dos povoamentos. Os dados são provenientes de 272 parcelas temporárias com idades variando de 3 a 18 anos. Foram testados 21 modelos tradicionais: 10 aritméticos e semilogarítmicos e 11 logarítmicos. Foram também desenvolvidas equações pelo processo Stepwise a partir de uma matriz de correlação. As equações ajustadas foram comparadas pelo Coeficiente de determinação ajustado (R²aj, Erro-padrão da estimativa percentual (Syx%, teste F e Distribuição gráfica de resíduos. Duas equações para cada caso foram selecionadas, sendo uma tradicional e a outra desenvolvida por Stepwise. Para a biomassa total, foram selecionadas a equação de Clutter e a equação aritmética desenvolvida por Stepwise; e para a biomassa da lenha, a equação da variável combinada, proposta por Spurr, e a equação aritmética de Stepwise. Por fim, realizou-se teste Qui-quadrado (x² a partir de uma amostra de 17 parcelas extraídas da base de dados, em que as quatro equações selecionadas foram consideradas válidas para a estimativa da biomassa total e da lenha dos bracatingais da região metropolitana de Curitiba.

  3. Revisión del género Salvia L. sect. Aethiopis Benth. (Lamiaceae en el Mediterráneo Occidental

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    Rosúa, J. L.

    1989-06-01

    Full Text Available A taxonomic revision of Salvia L. sect. Aethiopis Benth. in Western Mediterranean is presented. 4 species and 3 subspecies are recognized. A key to the species is given, together with the correct name, synonymy, description, type, chromosome number, distribution, ecology, phytosociology and a list of localities for each taxon. The new combination Salvia phlomoides Asso subsp. boissieri (Noë Rosúa & Blanca is proposed. The general distribution and the possible origin of the studied taxa is shortly discussed.

    Se revisa taxonómicamente la sect. Aethiopis Benth. del género Salvia L. en el Mediterráneo Occidental. Se reconocen 4 especies y 3 subespecies. Para cada taxon se indica el nombre correcto, sinonimia, descripción, tipo, número cromosómico, distribución, ecología, fitosociología y localidades estudiadas. Se propone la nueva combinación Salvia phlomoides Asso subsp. boissieri (Noë Rosúa & Blanca. Se establece la distribución actual de los táxones estudiados y se analiza su posible origen.

  4. Optimization of extracting technology for cytisine in Thermopsis chinensis Benth seeds%小叶野决明种子中金雀花碱提取工艺的优化

    Institute of Scientific and Technical Information of China (English)

    刘国宇; 周军辉; 谢斌; 王庆; 孙颖; 崔新爱

    2016-01-01

    采用正交实验设计优化了小叶野决明种子中金雀花碱的热回流法提取工艺。采用高效液相色谱法测定了提取样品中金雀花碱的含量。热回流法的最佳提取工艺为:药材粉末加10倍量的80%乙醇,回流提取1.5 h,提取3次。在此条件下,提取率可达1.68%。经验证,优化的提取工艺稳定可行,可作为小叶野决明种子中金雀花碱提取的新工艺。%The orthogonal test was used for optimizing the refluxing extraction technology for cytisine in Thermopsis chinensis Benth seeds.We detected the content of cytisine by high-performance liquid chroma-tography.Cytisine was extracted with 10 times 80% ethanol,refluxed for 1.5 h,and with 3 repolications under these condition,the yield up to 1.68%.The optimized technique is stable and can be used for ex-tracting cytisine from in Thermopsis chinensis Benth seeds.

  5. 鸡血藤红色素的提取与基本性质分析%Red Pigment Extraction from Millettia reticulata Benth and Physical and Chemical Properties of Pigment

    Institute of Scientific and Technical Information of China (English)

    许润; 刘建华; 杨广成; 李计龙; 杨迺嘉

    2012-01-01

    探讨鸡血藤红色素的最佳提取溶液,定性定量光、温度、pH值、氧化还原介质对红色素稳定性的影响。结果表明,鸡血藤红色素最佳提取溶剂为65%酸性乙醇(pH1.8),且色素为非花色苷类色素。室内自然光对色素影响不大,该红色素在20~60℃具有较好的稳定性,对氧化剂、还原剂反应敏感。%The red pigment was extracted from Millettia reticulata Benth and the physical and chemical properties of red pigment from Millettia reticulata Benth were investigated, the effects of light, temperature, pH value, oxide-reducing medium on pigment stability were studied. The results showed that the procedure of acid-alcohol extraction gave rather satisfactory results. The pigment does not belong to anthocyanins, and is stable below 20 -- 60 ~C, but its resistance to oxide and reductant is not good.

  6. Chemical composition and larvicidal activity of leaf essential oil from Clausena anisata (Willd.)Hook. f. exBenth (Rutaceae) against three mosquito species

    Institute of Scientific and Technical Information of China (English)

    Marimuthu Govindarajan

    2010-01-01

    Objective:To determine the mosquito larvicidal activity of leaf essential oil and their chemical constituents fromClausena anisata(C. anisata) (Willd.) Hook. f. ex Benth. against Culex quinquefasciatus, Aedes aegypti andAnopheles Stephensi.Methods:Essential oil was obtained by hydro-distillation and the chemical composition of the leaf essential oil was analyzed using gas chromatography-mass spectrometry. The mosquitoes were reared in the vector control laboratory and twenty late III instar larvae of three mosquito species were exposed to based on the wide range and narrow range tests, essential oil was tested at50, 100, 150, 200 and 250 ppm and each compound was tested at various concentration (5-75 ppm) and were assayed in the laboratory by using the protocol ofWHO 2005; the 24 hLC50 values of theC. anisata leaf essential oil and their major compounds were determined following Probit analysis.Results:The oil contained were mainly β-pinene (32.8%), sabinene(28.3%), germacrene-D (12.7%), estragole (6.4%) and linalool(5.9%). The essential oil from the leaves ofC. anisataexhibited significant larvicidal activity, with24 hLC50 values of140.96, 130.19 and119.59ppm, respectively. The five pure constituents extracted from theC. anisata leaf essential oil were also tested individually against three mosquito larvae. The LC50values of β-pinene, sabinene, germacrene-D, estragole and linalool appeared to be most effective againstAnopheles stephensi(LC50-23.17, 19.67, 16.95, 11.01, 35.17ppm) followed byAedes aegypti (LC50-27.69, 21.20,18.76, 12.70, 38.64 ppm) and Culex quinquefasciatus(LC50-32.23, 25.01, 21.28, 14.01, 42.28).Conclusions:The essential oil of C. anisata contains five major compounds and has remarkable larvicidal properties, which may be considered as a potent source for the production of natural larvicides.

  7. Sobre a botânica, a etnofarmacologia e a química de Calycophyllum spruceanum (Benth. Hook. f. ex K. Schum

    Directory of Open Access Journals (Sweden)

    A.B. SANTOS

    2016-01-01

    Full Text Available RESUMO O fato de possuir espécies com potencial econômico ilimitado, faz com que o ambiente amazônico se torne alvo constante da biopirataria e da extração predatória dos recursos. Muitas espécies deste ambiente sequer possuem catalogação e, mesmo assim, encontram risco eminente de desaparecimento. Neste âmbito, trabalhos que reúnam dados sobre estas espécies possuem grande valor científico, cultural e econômico, sendo este o estímulo que desencadeou a produção desta revisão. Aqui a espécie Calycophyllum spruceanum (Benth. Hook. f. ex K. Schum., uma Rubiaceae amazônica, é contemplada por meio de características botânicas, indicações etnofarmacológicas e propriedades químicas. Popularmente conhecida como mulateiro, a espécie é frequentemente receitada na etnomedicina como cicatrizante e rejuvenescedor, além de ser usada no controle de manchas de pele. Como peculiaridade botânica, mulateiro apresenta tronco retilíneo com epiderme fina esverdeada que evolui para uma periderme castanho-escuro, que é anualmente renovada. Em termos químicos, há destaque para a presença de alcalóides, taninos e, sobretudo, secoiridóides (7-metoxididerrosideo, 6´-acetil-β-D-glucopiranosildiderrosideo e 8-0-tigloildiderrosideo são peculiares à espécie. Mesmo com propriedades fotoprotetoras comprovadas, C. spruceanum ainda carece de pesquisas, sobretudo àquelas voltadas para a produção ex situ da planta e àquelas que demonstrem a relação entre a ecologia da planta e a produção de metabólitos funcionais para a indústria.

  8. Soil amendment effects on the exotic annual grass Bromus tectorum L. and facilitation of its growth by the native perennial grass Hilaria jamesii (Torr.) Benth

    Science.gov (United States)

    Belnap, J.; Sherrod, S.K.

    2009-01-01

    Greenhouse experiments were undertaken to identify soil factors that curtail growth of the exotic annual grass Bromus tectorum L. (cheatgrass) without significantly inhibiting growth of native perennial grasses (here represented by Hilaria jamesii [Torr.] Benth). We grew B. tectorum and H. jamesii alone (monoculture pots) and together (combination pots) in soil treatments that manipulated levels of soil phosphorus, potassium, and sodium. Hilaria jamesii showed no decline when its aboveground biomass in any of the applied treatments was compared to the control in either the monoculture or combination pots. Monoculture pots of B. tectorum showed a decline in aboveground biomass with the addition of Na2HPO4 and K2HPO4. Interestingly, in pots where H. jamesii was present, the negative effect of these treatments was ameliorated. Whereas the presence of B. tectorum generally decreased the aboveground biomass of H. jamesii (comparing aboveground biomass in monoculture versus combination pots), the presence of H. jamesii resulted in an enhancement of B. tectorum aboveground biomass by up to 900%. We hypothesize that B. tectorum was able to obtain resources from H. jamesii, an action that benefited B. tectorum while generally harming H. jamesii. Possible ways resources may be gained by B. tectorum from native perennial grasses include (1) B. tectorum is protected from salt stress by native plants or associated soil biota; (2) when B. tectorum is grown with H. jamesii, the native soil biota is altered in a way that favors B. tectorum growth, including B. tectorum tapping into the mycorrhizal network of native plants and obtaining resources from them; (3) B. tectorum can take advantage of root exudates from native plants, including water and nutrients released by natives via hydraulic redistribution; and (4) B. tectorum is able to utilize some combination of the above mechanisms. In summary, land managers may find adding soil treatments can temporarily suppress B. tectorum

  9. 吴茱萸不同炮制方法对抗炎镇痛作用的影响研究%Study on Analgesic and Anti - Inflammatory Effects of Different Kinds of Evodia Rutaecarpa (Juss.)Benth.

    Institute of Scientific and Technical Information of China (English)

    杨磊; 黄开颜; 陈兴; 杨晖; 李康; 张志国

    2013-01-01

    目的 观察吴茱萸不同炮制品的抗炎镇痛作用.方法 采用小鼠热板法和扭体法,观察吴茱萸不同炮制品的镇痛作用;采用耳肿法观察吴茱萸不同炮制品的抗炎作用.结果 吴茱萸不同炮制品能明显提高热板和扭体试验小鼠的痛阈值,其中以砂烫盐炙组作用较强;对二甲苯所致的小鼠耳廓肿胀有明显的抑制作用,其中以砂烫组作用较强.结论 吴茱萸不同炮制品均有显著的抗炎、镇痛作用,其中以砂烫组和砂烫盐炙组作用较强.%Objective To study analgesic and anti - inflammatory effects of different processed products of Evodia rutaecarpa (Juss. ) Benth. Methods The analgesic effect was tested by hot plate method and writhing body method in mice. The anti - inflammatory effect was tested by ear swollen method in mice. Results For the pain caused by hot plate method and writhing body method, the different processed products of Evodia rutaecarpa{Juss. ) Benth. have obviously increased threshold of pain in mice. Among these products, the group of stir - baked in sand with processed salty water shows more intensive effect,which has substantially inhibiting effects on mice s ear swelling caused by dimethylbenzene. The stir - baked in sand is more intensive. Conclusion Different processed products of Evodia rutaecarpa (Juss. ) Benth. have marked analgesic and anti - inflammatory effects, of which the stir - baked in sand one and stir - baked in sand wTith processed salty water one are more intensive.

  10. Morphogenesis and Developmental Law of Glandular Hairs of Elsholtzia stauntoni Benth%木香薷腺毛形态结构发生发育规律的研究

    Institute of Scientific and Technical Information of China (English)

    朱广龙; 赵挺; 康冬冬; 魏学智

    2011-01-01

    采用常规石蜡切片法及扫描电镜技术对木香薷(Elsholtzia stauntoni Benth)腺毛发生发育及其规律进行了研究.结果表明:木香薷表皮上主要有两种表皮毛:无分泌细胞的表皮毛与有分泌细胞的腺毛.前者包括单细胞乳头状毛、2~3细胞管状毛、分枝状毛及多细胞管状毛;后者包括头状腺毛与盾状腺毛.成熟头状腺毛头部由1、2或4个分泌细胞构成,头部呈圆球形或半圆球形;成熟盾状腺毛头部由8~12个分泌细胞构成,分泌细胞横向扩展形成盾状头部.木香薷腺毛主要在茎端幼叶处大量发生,从茎端第一对幼叶处开始产生;从幼叶期到成熟期均有腺毛发生,大部分腺毛在幼叶期发生发育,只有极少部分在叶的成熟期进行发生发育.%The paraffin sectionning and scanning electron microscopy ( SEM ) were used to study the morphogenesis and law of development of Elsholtzia stauntoni Benth. The results showed that glandular hairs of E. Stauntoni Benth has two main types of trichomes: trichomes without secreting cells and trichomes with secreting cells. The former include: single-cell mastoid trichomes ,2-3 cells tubular trichomes ,2-3 cells ramose trichomes and multicellular tubular trichomes. The latter include peltate and capitate hairs. The mature capitate glandular hairs comprise one, two or four secretory cells and its head is spherical or half spherical. The head of mature peltate glandular hairs comprises eight to twelve secretory cells and the secretory cells expand to a shield-shaped head. The glandular hairs of E. Stauntoni Benth mainly occur gready on younger leaves and start to generate from the first pair of younger leaves of stem apex. The morphogenesis of new glandular hairs can be observed to occur from young leaves stage to mature stage, however, only a relatively small amount occurs in maturation stage.

  11. Estudo fitoquímico e análise mutagênica das folhas e inflorescências de Erythrina mulungu (Mart. ex Benth. através do teste de micronúcleo em roedores Phytochemical and mutagenic analysis of leaves and inflorescences of Erythrina mulungu (Mart. Ex Benth through micronucleus test in rodents

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    A.P De Bona

    2012-01-01

    Full Text Available Este trabalho teve como objetivo investigar a composição química, estabelecer a dose letal média (DL50 e avaliar os potenciais efeitos mutagênicos do extrato hidroalcoólico de folhas e inflorescências de Erythrina mulungu Mart. ex Benth por meio do teste de micronúcleo em medula óssea de camundongos. Os ensaios fitoquímicos foram realizados através de reações preliminares com mudança de coloração e/ou formação de precipitado; a DL50, por meio da administração intraperitoneal de três concentrações dos extratos, avaliando-se o número de óbitos após 48 horas e o teste de micronúcleo foi feito por meio do método do esfregaço, após exposição dos animais a cinco dias de tratamento. Os resultados fitoquímicos demonstraram presença de açúcares redutores, fenóis e taninos, proteínas e aminoácidos, flavonóides, alcalóides, depsídeos e depsidonas e derivados de cumarina em ambos os órgãos; saponinas espumídicas e esteróides e triterpenóides nas folhas e glicosídeos cardiotônicos e antraquinônicos e alcalóides nas inflorescências. Para a DL50 a folha demonstrou-se atóxica e a inflorescência moderadamente tóxica. Para o teste de micronúcleo, os resultados indicaram ausência de citotoxicidade e genotoxicidade dose-dependente para as folhas e independente da dose para as inflorescências. Assim, esses resultados sugerem que a planta, nas condições analisadas, possui potencial para induzir danos ao DNA.This study aimed to investigate the chemical composition, to establish the mean lethal dose (LD50 and to assess the potential mutagenic effects of hydroalcoholic extract of leaves and inflorescences of Erythrina mulungu Mart. ex Benth by using micronucleus test in bone marrow of mice. Phytochemical assays were carried out through preliminary reactions with color change and/or precipitate formation; the LD50 was obtained by intraperitoneal administration of three concentrations of the extracts, assessing

  12. Influência da escarificação e da temperatura sobre a germinação de sementes de Samanea tubulosa (Benth. Barneby & J.W. Grimes (sete cascas Influence of scarification and temperature on seed germination of Samanea tubulosa (Benth. Barneby & J.W. Grimes (seven shells

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    Roseli Muniz Giachini

    2010-03-01

    Full Text Available Samanea tubulosa (Benth. Barneby & J.W Grimes (sete cascas é uma planta arbórea nativa do Pantanal Matogrossense, cujas sementes possuem dormência provavelmente causada pela impermeabilidade do tegumento à água. O presente trabalho foi conduzido com o objetivo de comparar a eficiência da escarificação mecânica e química para superar a dormência tegumentar e o efeito da temperatura sobre a germinação de sementes de sete cascas. Foram empregados quatro diferentes procedimentos de escarificação: testemunha (sem escarificação; a escarificação mecânica; a escarificação com ácido sulfúrico durante cinco minutos e a escarificação com ácido sulfúrico durante dez minutos. Os níveis de temperatura empregados foram de 20, 25, 30 e 35ºC. A semeadura foi realizada em papel toalha germitest, na forma de rolo. O delineamento experimental adotado foi o inteiramente casualizado com os tratamentos em esquema fatorial 4x4. Para cada tratamento, foram utilizadas quatro repetições de 25 sementes. Foi avaliada a porcentagem de germinação das sementes, utilizado como critério emissão da raiz primária com 2 mm de comprimento. Foi observado que a espécie S. tubulosa possui dormência tegumentar causada pela impermeabilidade do tegumento a água. As escarificações química com ácido sulfúrico por cinco e dez minutos foram eficientes para superação da dormência e as combinações de escarificação com temperatura que promoveram maiores porcentagens de germinação para a espécie foram a escarificação com imersão em ácido sulfúrico durante cinco e dez minutos e as temperaturas de 25, 30 e 35ºC.Samanea tubulosa (Benth. Barneby & JW Grimes (seven shells is a tree plant native to the Mato Grosso Pantanal, whose seeds have dormancy probably caused by impregnability of its integument to water. This work was carried out to compare the efficiency of mechanical and chemical scarification for overcoming integumentary dormancy

  13. Protective effects of alginate-chitosan microspheres loaded with alkaloids from Coptis chinensis Franch. and Evodia rutaecarpa (Juss.) Benth. (Zuojin Pill) against ethanol-induced acute gastric mucosal injury in rats.

    Science.gov (United States)

    Wang, Qiang-Song; Zhu, Xiao-Ning; Jiang, Heng-Li; Wang, Gui-Fang; Cui, Yuan-Lu

    2015-01-01

    Zuojin Pill (ZJP), a traditional Chinese medicine formula, consists of Coptis chinensis Franch. and Evodia rutaecarpa (Juss.) Benth. in a ratio of 6:1 (w/w) and was first recorded in "Danxi's experiential therapy" for treating gastrointestinal disorders in the 15th century. However, the poor solubility of alkaloids from ZJP restricted the protective effect in treating gastritis and gastric ulcer. The aim of the study was to investigate the protective mechanism of mucoadhesive microspheres loaded with alkaloids from C. chinensis Franch. and E. rutaecarpa (Juss.) Benth. on ethanol-induced acute gastric mucosal injury in rats. Surface morphology, particle size, drug loading, encapsulation efficiency, in vitro drug release, mucoadhesiveness, and fluorescent imaging of the microspheres in gastrointestinal tract were studied. The results showed that the mucoadhesive microspheres loaded with alkaloids could sustain the release of drugs beyond 12 hours and had gastric mucoadhesive property with 82.63% retention rate in vitro. The fluorescence tracer indicated high retention of mucoadhesive microspheres within 12 hours in vivo. The mucoadhesive microspheres loaded with alkaloids could reduce the gastric injury by decreasing the mucosal lesion index, increasing the percentage of inhibition and increasing the amount of mucus in the gastric mucosa in an ethanol-induced gastric mucosal injury rat model. Moreover, the mucoadhesive microspheres loaded with alkaloids reduce the inflammatory response by decreasing the levels of tumor necrosis factor-α (TNF-α), interleukin 1β (IL-1β), downregulating the mRNA expression of inducible nitric oxide synthase, TNF-α, and IL-1β in gastric mucosa. All the results indicate that mucoadhesive microspheres loaded with alkaloids could not only increase the residence time of alkaloids in rat stomach, but also exert gastroprotective effects through reducing the inflammatory response on ethanol-induced gastric mucosal damage. Thus, these

  14. 中国球兰属新记录植物——长瓣球兰(Hoya acuminata)%Hoya acuminata (Wight) Benth.ex Hook.f.(Apocynaceae, Asclepiadoideae),A Newly Recorded Species from China

    Institute of Scientific and Technical Information of China (English)

    桂凌健; 彭玉德; 黄宝优; 兰小中; 余丽莹

    2017-01-01

    报道了夹竹桃科萝藦亚科长瓣球兰[Hoya acuminata (Wight) Benth.ex Hook.f.]在中国的新分布记录.长瓣球兰过去记录在不丹、印度东北部(Sikkim, Khasia)和缅甸,2015年在中国西南部的西藏墨脱县内、海拔1 577 m的山林树上发现长瓣球兰.该物种与景洪球兰相近,但前者枝条光滑无毛,每个花序着花3~5朵,花冠直径达5 cm,花冠裂片长2 cm.凭证标本存放于广西药用植物园标本馆(GXMG).%Hoya acuminata (Wight) Benth.ex Hook.f.is reported as a new record species of Apocynaceae, Asclepiadoideae from China.H.acuminata has been recorded in Bhutan, Sikkim, Khasia and Myanmar and it was found on a tree of dense moist forest in Medog County, China at the altitude of 1 577 m in 2015.It is related to H.chinghungensis, but differs in its branches quite glabrous, umbels 3-5 flowered, with corollas up to 5 cm in diameter and lobes 2 cm.The voucher specimens are preserved in the herbarium of Guangxi Botanical Garden of Medicinal Plants (GXMG).

  15. Diversity and symbiotic effectiveness of beta-rhizobia isolated from sub-tropical legumes of a Brazilian Araucaria Forest.

    Science.gov (United States)

    Lammel, Daniel R; Cruz, Leonardo M; Carrer, Helaine; Cardoso, Elke J B N

    2013-12-01

    While the occurrence of Betaproteobacteria occupying the nodules of tropical legumes has been shown, little is known about subtropical areas. Araucaria Forest is a subtropical endangered ecosystem, and a better understanding of the legume-rhizobial symbionts may allow their use in land reclamation. The 16S rRNA gene of bacteria isolated from nine leguminous species was sequenced and their nodulation tested in Mimosa scabrella and Phaseolus vulgaris. 196 isolates were identified as eight genotypes: Pantoea, Pseudomonas, Bradyrhizobium sp1-2, Rhizobium, and Burkholderia sp1-3. The majority of the isolates from native plants (87 %) were taxonomically related to β-rhizobia, namely Burkholderia, however the legumes Galactia crassifolia and Collea speciosa were nodulated by both α and β-rhizobia, and Acacia dealbata, an exotic plant, only by α-rhizobia. The nifH genes of some isolates were sequenced and N-fixing potential shown by the acetylene reduction test. Most of the isolates nodulated the test plants, some were effective in M. scabrella, but all presented low efficiency in the exotic promiscuous legume P. vulgaris. Pantoea and Pseudomonas did not nodulate and probably are endophytic bacteria. The presented data shows diversity of α, β and γ-Proteobacteria in nodules of subtropical legumes, and suggests host specificity with β-rhizobia. Potential isolates were found for M. scabrella, indicating that a high N-fixing strain may be further inoculated in plants for use in reforestation.

  16. Genetic diversity of symbiotic Paraburkholderia species isolated from nodules of Mimosa pudica (L.) and Phaseolus vulgaris (L.) grown in soils of the Brazilian Atlantic Forest (Mata Atlântica).

    Science.gov (United States)

    Dall'Agnol, Rebeca Fuzinatto; Bournaud, Caroline; de Faria, Sérgio Miana; Béna, Gilles; Moulin, Lionel; Hungria, Mariangela

    2017-04-01

    Some species of the genus Paraburkholderia that are able to nodulate and fix nitrogen in symbiosis with legumes are called β-rhizobia and represent a group of ecological and biotechnological importance. We used Mimosa pudica and Phaseolus vulgaris to trap 427 rhizobial isolates from rhizospheric soil of Mimoseae trees in the Brazilian Atlantic Forest. Eighty-four representative strains were selected according to the 16S rRNA haplotypes and taxonomically characterized using a concatenated 16S rRNA-recA phylogeny. Most strains were assembled in the genus Paraburkholderia, including Paraburkholderia sabiae and Pa. nodosa. Mesorhizobium (α-rhizobia) and Cupriavidus (β-rhizobia) were also isolated, but in smaller proportions. Multilocus sequence analysis and BOX-PCR analyses indicated that six clusters of Paraburkholderia represent potential new species. In the phylogenetic analysis of the nodC gene, the majority of the strains were positioned in the same groups as in the 16S rRNA-recA tree, indicative of stability and vertical inheritance, but we also identified horizontal transfer of nodC in Pa. sabiae. All α- and β-rhizobial species were trapped by both legumes, although preferences of the host plants for specific rhizobial species have been observed. © FEMS 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  17. Efeito da temperatura e do teor de umidade na iniciação e desenvolvimento do rizoma de Kohleria eriantha (Benth. Hanst. (Gesneriaceae Effect of temperature and the water content in the initiation and developmental of the rhizome of Kohleria eriantha (Benth. Hanst. (Gesneriaceae

    Directory of Open Access Journals (Sweden)

    Julieta Andrea Silva de Almeida

    2004-12-01

    Full Text Available Kohleria eriantha (Benth. Hanst. pertence à família Gesneriaceae e possui órgão subterrâneo, que está associado à reprodução vegetativa. Este órgão apresenta gemas envoltas por folhas modificadas, as quais armazenam amido. Em seções do rizoma (1,5 a 2,0cm compr. contendo seis gemas, só uma gema geralmente brota. Esta pode diferenciar-se em dois padrões morfológicos: parte aérea ou rizoma. Em seções mantidas em substrato com baixo teor de umidade (1mL de água ou em sua ausência, houve brotação do padrão rizoma, em seções em substrato com elevado teor de umidade (12mL de água, brotação do padrão parte aérea. A temperatura de 20ºC também favoreceu a brotação do padrão rizoma, independente do volume de água do substrato. Seções também desenvolveram o padrão rizoma em substrato com adição de solução de polietilenoglicol 6000 (PEG, nas concentrações de 161,2; 235,2 e 340,0g/L, que geraram os potenciais de -3, -6 e -12 MPa, respectivamente. Seções mantidas em substrato com baixo teor de umidade (1mL de água apresentaram redução de massa seca e elevada concentração osmótica em relação àquelas em substrato com elevado teor de umidade. Verificou-se que a formação do padrão rizoma foi influenciada pelos fatores teor de umidade e temperatura. Sugere-se que a brotação do padrão rizoma foi induzida pelo baixo potencial hídrico nas seções, quando mantidas em substrato com baixo teor de umidade. Além disto, evidenciou-se que as gemas do rizoma de Kohleria eriantha apresentam elevado grau de plasticidade.Kohleria eriantha (Benth. Hanst is a plant belonging to the family Gesneriaceae, with an underground organ, which is associated with vegetative reproduction. This organ is a rhizome, whose stem bears buds covered with modified leaves that store up starch. In small sections of this rhizome, containing six buds (1.5 to 2.0cm long, only one bud sprouted. The sprouted bud could be differentiated

  18. Características físico-químicas y composición de ácidos grasos del aceite crudo extraído de residuos de mora (Rubus glaucus Benth

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    Moreno-Álvarez, Mario José

    2003-09-01

    Full Text Available In this research the physicochemical characteristics and fatty acids composition of oil extracted from blackberry (Rubus glaucus Benth residue were determined. Mature fruits from ‘‘La Colonia Tovar’’, Aragua state, Venezuela harvested on April 2001, were processed to obtain seeds and residues of pulp. This residue was extracted with n-hexane (Soxhlet method. The crude oil was assayed by means of COVENIN and AOCS methods. The results showed: Iodine index 160.16 cg I2/g, refraction index to 25ºC 1.4780 , saponification value 193.76 mg K0H/g, peroxide value 30.40 meq 02/kg, free fatty acidity 2.83% (oleic acid, insaponifiable value 2.77%, phosphorus content 0.22% and stability 3.09 h (AOM. The major fatty acids found were oleic acid (55.39% and linoleic acid (29.51%.El objetivo de esta investigación fue caracterizar físico-químicamente el aceite extraído de residuos de mora (Rubus glaucus Benth y determinar su composición de ácidos grasos. Frutos maduros procedentes de ‘‘La Colonia Tovar’’, estado Aragua, Venezuela perteneciente a la cosecha Abril 2001, fueron procesados para obtener residuos constituidos por semillas y restos de pulpa. Los residuos fueron sometidos a un proceso de extracción mediante equipo Soxhlet utilizando como solvente n-hexano. El aceite crudo extraído fue caracterizado mediante normas COVENIN y AOCS. Se determinaron valores de índice de Iodo 160,16 cg I2/g; índice de refracción 1,4780 a 25ºC; índice de saponificación 193,76 mg KOH/g; índice de peróxidos 30,40 meq O2/kg; acidez libre oleica 2,83 %; materia insaponificable 2,77 %; fósforo 0,22 % y estabilidad AOM de 3,09 horas. Los ácidos grasos mayoritarios encontrados fueron el ácido oleico (55,39% y el ácido linoleico (29,51%.

  19. Inhibitory Effect of Total Sterol of Salvia Chinensis Benth.Against SGC-7901 Cells%石见穿总甾醇对 SGC -7901细胞的影响

    Institute of Scientific and Technical Information of China (English)

    魏清; 王燕燕; 周政涛; 柳蔚

    2015-01-01

    s:Objective To observe the effect total sterol of Salvia Chinensis Benth.on growth of human gastric carcinoma line SGC-7901, and explore its possible mechanism.Methods To observe the effect of total sterol of Salvia Chinensis Benth.on proliferation of SGC-7901 cells by MTT method, and detect the mitochondrial membrane potential and cell cycle.Results The total sterol can inhibit the pro-liferation of SGC-7901 cells significantly in a dose and time dependent manner, and it has no notable effect on the mitochondrial membrane potential of SGC-7901 cells, and the results of flow cytometry showed the total sterol induce the SGC-7901 cells to necrosis.Conclusions The total sterol inhibit human gastric cancer cells SGC-7901 proliferation by induction of cell necrosis.%目的:观察石见穿总甾醇对人胃癌细胞SGC-7901生长的影响,并探讨其可能的作用机制。方法用MTT法观察石见穿总甾醇对SGC-7901细胞增殖的影响,用荧光酶标仪检测对线粒体膜电位的影响,用流式细胞术检测细胞周期的影响。结果石见穿总甾醇能显著抑制SGC-7901细胞的增殖,具有剂量依赖性和时间依赖性,对SGC-7901细胞的线粒体膜电位没有显著影响,流式细胞术结果表明石见穿总甾醇处理后SGC-7901细胞以坏死为主。结论石见穿总甾醇对人胃癌细胞SGC-7901的增殖具有显著抑制作用,其作用机制可能是诱导细胞坏死有关。

  20. Protective effects of alginate–chitosan microspheres loaded with alkaloids from Coptis chinensis Franch. and Evodia rutaecarpa (Juss. Benth. (Zuojin Pill against ethanol-induced acute gastric mucosal injury in rats

    Directory of Open Access Journals (Sweden)

    Wang QS

    2015-11-01

    Full Text Available Qiang-Song Wang,1,2,* Xiao-Ning Zhu,1,* Heng-Li Jiang,1,* Gui-Fang Wang,3 Yuan-Lu Cui1 1Tianjin State Key Laboratory of Modern Chinese Medicine, Research Center of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 2Institute of Biomedical Engineering, Chinese Academy of Medical Science & Peking Union Medical College, 3Pharmacy Department, Baokang Hospital, Tianjin University of Traditional Chinese Medicine, Tianjin, People’s Republic of China *These authors contributed equally to this work Abstract: Zuojin Pill (ZJP, a traditional Chinese medicine formula, consists of Coptis chinensis Franch. and Evodia rutaecarpa (Juss. Benth. in a ratio of 6:1 (w/w and was first recorded in “Danxi’s experiential therapy” for treating gastrointestinal disorders in the 15th century. However, the poor solubility of alkaloids from ZJP restricted the protective effect in treating gastritis and gastric ulcer. The aim of the study was to investigate the protective mechanism of mucoadhesive microspheres loaded with alkaloids from C. chinensis Franch. and E. rutaecarpa (Juss. Benth. on ethanol-induced acute gastric mucosal injury in rats. Surface morphology, particle size, drug loading, encapsulation efficiency, in vitro drug release, mucoadhesiveness, and fluorescent imaging of the microspheres in gastrointestinal tract were studied. The results showed that the mucoadhesive microspheres loaded with alkaloids could sustain the release of drugs beyond 12 hours and had gastric mucoadhesive property with 82.63% retention rate in vitro. The fluorescence tracer indicated high retention of mucoadhesive microspheres within 12 hours in vivo. The mucoadhesive microspheres loaded with alkaloids could reduce the gastric injury by decreasing the mucosal lesion index, increasing the percentage of inhibition and increasing the amount of mucus in the gastric mucosa in an ethanol-induced gastric mucosal injury rat model. Moreover, the

  1. Modifications of the chemical structure of phenolics differentially affect physiological activities in pulvinar cells of Mimosa pudica L. II. Influence of various molecular properties in relation to membrane transport.

    Science.gov (United States)

    Rocher, Françoise; Roblin, Gabriel; Chollet, Jean-François

    2017-03-01

    Early prediction of compound absorption by cells is of considerable importance in the building of an integrated scheme describing the impact of a compound on intracellular biological processes. In this scope, we study the structure-activity relationships of several benzoic acid-related phenolics which are involved in many plant biological phenomena (growth, flowering, allelopathy, defense processes). Using the partial least squares (PLS) regression method, the impact of molecular descriptors that have been shown to play an important role concerning the uptake of pharmacologically active compounds by animal cells was analyzed in terms of the modification of membrane potential, variations in proton flux, and inhibition of the osmocontractile reaction of pulvinar cells of Mimosa pudica leaves. The hydrogen bond donors (HBD) and hydrogen bond acceptors (HBA), polar surface area (PSA), halogen ratio (Hal ratio), number of rotatable bonds (FRB), molar volume (MV), molecular weight (MW), and molar refractivity (MR) were considered in addition to two physicochemical properties (logD and the amount of non-dissociated form in relation to pKa). HBD + HBA and PSA predominantly impacted the three biological processes compared to the other descriptors. The coefficient of determination in the quantitative structure-activity relationship (QSAR) models indicated that a major part of the observed seismonasty inhibition and proton flux modification can be explained by the impact of these descriptors, whereas this was not the case for membrane potential variations. These results indicate that the transmembrane transport of the compounds is a predominant component. An increasing number of implicated descriptors as the biological processes become more complex may reflect their impacts on an increasing number of sites in the cell. The determination of the most efficient effectors may lead to a practical use to improve drugs in the control of microbial attacks on plants.

  2. 胡蔓藤中非生物碱类成分的分离与鉴定(Ⅲ)%Isolation and identification of the non-alkaloid constituents from whole plant of Gelsemium elegans Benth.(Ⅲ)

    Institute of Scientific and Technical Information of China (English)

    赵庆春; 华威; 付艳辉; 杜占权; 郭涛; 吴立军

    2010-01-01

    目的 研究胡蔓藤(Gelsemium elegans Benth.)中的化学成分.方法 采用硅胶柱色谱和ODS柱色谱进行分离,Sephadex LH-20及制备液相进行纯化,根据理化性质和光谱分析进行结构鉴定.结果 分离鉴定了8个化合物,分别为(+)-8-hydroxypinoresinol(1)、cleomiscosin C(2)、cleomiscosin A(3)、3,4-二羟基苯甲醛(3,4-dihydroxyphenyl aldehyde,4)、咖啡酸(caffeic acid,5)、1-D-咖啡酰基奎宁酸(1-O-caffeoylquinic acid,6)、4-O-咖啡酰基奎宁酸(4-O-caffeoylquinic acid,7)、1-O-咖啡酰基奎宁酸甲酯(1-O-caffeoylquinic acid methyl ester,8).结论 化合物1-8为首次从该属植物中分离得到.

  3. Chemical composition of total flavonoids from Salvia chinensia Benth and their pro-apoptotic effect on hepatocellular carcinoma cells: potential roles of suppressing cellular NF-κB signaling.

    Science.gov (United States)

    Xiang, Meixian; Su, Hanwen; Hu, Yajing; Hu, Yun; Yang, Tianming; Shu, Guangwen

    2013-12-01

    Salvia chinensia Benth (S. chinensia) is a medical plant that has been traditionally applied for centuries in the treatment of malignant diseases including hepatocellular carcinoma (HCC). However, the scientific basis underlying its anti-HCC activity has not been fully established. In this study, the chemical profiles of total flavonoids from S. chinensia (TFSC) were explored. Thirteen compounds which constituted the major components of TFSC were separated and identified. Flow cytometry analysis and caspase activity assays showed that TFSC dose-dependently induced HepG2 and Huh-7 HCC cell apoptosis. TFSC was also shown to substantially suppress NF-κB activity in HCC cells. Moreover, TFSC significantly repressed transplanted murine H22 ascitic hepatic cancer cell growth i