Pavlov's methodological behaviorism as a pre-Socratic contribution of the melding of the differential and experimental psychology.

Science.gov (United States)

Furedy, John J

2003-11-01

The differential/experimental distinction that Cronbach specified is important because any adequate account of psychological phenomena requires the recognition of the validity of both approaches, and a meaningful melding of the two. This paper suggests that Pavlov's work in psychology, based on earlier traditions of inquiry that can be traced back to the pre-Socratics, provides a potential way of achieving this melding, although such features as systematic rather than anecdotal methods of observation need to be added. Pavlov's methodological behaviorist approach is contrasted with metaphysical behaviorism (as exemplified explicitly in Watson and Skinner, and implicitly in the computer-metaphorical, information-processing explanations employed by current "cognitive" psychology). A common feature of the metaphysical approach is that individual-differences variables like sex are essentially ignored, or relegated to ideological categories such as the treatment of sex as merely a "social construction." Examples of research both before and after the "cognitive revolution" are presented where experimental and differential methods are melded, and individual differences are treated as phenomena worthy of investigation rather than as nuisance factors that merely add to experimental error.

Implementation analysis of lean enablers for managing engineering programs

DEFF Research Database (Denmark)

von Arnim, Joachim; Oehmen, Josef; Rebentisch, Eric

2014-01-01

This paper presents research to improve the applicability of the Lean Enablers and consists of two parts. The first is a case study of a very successful project management maturity improvement initiative at Siemens Industry Sector’s Industry Automation division in the US. It views the initiative...... from the perspective of the Lean Enablers [Oehmen 2012] and is based on information from [Sopko 2012a], [Sopko 2012b], [Sopko 2010], [Sopko 2009], interviews, internal documentation, and the used MSP program management methodology [UK 2011]. The analysis of Lean Enablers incorporated in the MSP...

A reconstructed melanoma data set for evaluating differential treatment benefit according to biomarker subgroups

Directory of Open Access Journals (Sweden)

Jaya M. Satagopan

2017-06-01

Full Text Available The data presented in this article are related to the research article entitled “Measuring differential treatment benefit across marker specific subgroups: the choice of outcome scale” (Satagopan and Iasonos, 2015 [1]. These data were digitally reconstructed from figures published in Larkin et al. (2015 [2]. This article describes the steps to digitally reconstruct patient-level data on time-to-event outcome and treatment and biomarker groups using published Kaplan-Meier survival curves. The reconstructed data set and the corresponding computer programs are made publicly available to enable further statistical methodology research.

Differential equations

CERN Document Server

Barbu, Viorel

2016-01-01

This textbook is a comprehensive treatment of ordinary differential equations, concisely presenting basic and essential results in a rigorous manner. Including various examples from physics, mechanics, natural sciences, engineering and automatic theory, Differential Equations is a bridge between the abstract theory of differential equations and applied systems theory. Particular attention is given to the existence and uniqueness of the Cauchy problem, linear differential systems, stability theory and applications to first-order partial differential equations. Upper undergraduate students and researchers in applied mathematics and systems theory with a background in advanced calculus will find this book particularly useful. Supplementary topics are covered in an appendix enabling the book to be completely self-contained.

Unattended Monitoring System Design Methodology

International Nuclear Information System (INIS)

Drayer, D.D.; DeLand, S.M.; Harmon, C.D.; Matter, J.C.; Martinez, R.L.; Smith, J.D.

1999-01-01

A methodology for designing Unattended Monitoring Systems starting at a systems level has been developed at Sandia National Laboratories. This proven methodology provides a template that describes the process for selecting and applying appropriate technologies to meet unattended system requirements, as well as providing a framework for development of both training courses and workshops associated with unattended monitoring. The design and implementation of unattended monitoring systems is generally intended to respond to some form of policy based requirements resulting from international agreements or domestic regulations. Once the monitoring requirements are established, a review of the associated process and its related facilities enables identification of strategic monitoring locations and development of a conceptual system design. The detailed design effort results in the definition of detection components as well as the supporting communications network and data management scheme. The data analyses then enables a coherent display of the knowledge generated during the monitoring effort. The resultant knowledge is then compared to the original system objectives to ensure that the design adequately addresses the fundamental principles stated in the policy agreements. Implementation of this design methodology will ensure that comprehensive unattended monitoring system designs provide appropriate answers to those critical questions imposed by specific agreements or regulations. This paper describes the main features of the methodology and discusses how it can be applied in real world situations

Human mesenchymal stem cells self-renew and differentiate according to a deterministic hierarchy.

Directory of Open Access Journals (Sweden)

Rahul Sarugaser

Full Text Available BACKGROUND: Mesenchymal progenitor cells (MPCs have been isolated from a variety of connective tissues, and are commonly called "mesenchymal stem cells" (MSCs. A stem cell is defined as having robust clonal self-renewal and multilineage differentiation potential. Accordingly, the term "MSC" has been criticised, as there is little data demonstrating self-renewal of definitive single-cell-derived (SCD clonal populations from a mesenchymal cell source. METHODOLOGY/PRINCIPAL FINDINGS: Here we show that a tractable MPC population, human umbilical cord perivascular cells (HUCPVCs, was capable of multilineage differentiation in vitro and, more importantly, contributed to rapid connective tissue healing in vivo by producing bone, cartilage and fibrous stroma. Furthermore, HUCPVCs exhibit a high clonogenic frequency, allowing us to isolate definitive SCD parent and daughter clones from mixed gender suspensions as determined by Y-chromosome fluorescent in situ hybridization. CONCLUSIONS/SIGNIFICANCE: Analysis of the multilineage differentiation capacity of SCD parent clones and daughter clones enabled us to formulate a new hierarchical schema for MSC self-renewal and differentiation in which a self-renewing multipotent MSC gives rise to more restricted self-renewing progenitors that gradually lose differentiation potential until a state of complete restriction to the fibroblast is reached.

An IT-enabled supply chain model: a simulation study

Science.gov (United States)

Cannella, Salvatore; Framinan, Jose M.; Barbosa-Póvoa, Ana

2014-11-01

During the last decades, supply chain collaboration practices and the underlying enabling technologies have evolved from the classical electronic data interchange (EDI) approach to a web-based and radio frequency identification (RFID)-enabled collaboration. In this field, most of the literature has focused on the study of optimal parameters for reducing the total cost of suppliers, by adopting operational research (OR) techniques. Herein we are interested in showing that the considered information technology (IT)-enabled structure is resilient, that is, it works well across a reasonably broad range of parameter settings. By adopting a methodological approach based on system dynamics, we study a multi-tier collaborative supply chain. Results show that the IT-enabled supply chain improves operational performance and customer service level. Nonetheless, benefits for geographically dispersed networks are of minor entity.

Methodological guidelines

Energy Technology Data Exchange (ETDEWEB)

Halsnaes, K.; Callaway, J.M.; Meyer, H.J.

1999-04-01

The guideline document establishes a general overview of the main components of climate change mitigation assessment. This includes an outline of key economic concepts, scenario structure, common assumptions, modelling tools and country study assumptions. The guidelines are supported by Handbook Reports that contain more detailed specifications of calculation standards, input assumptions and available tools. The major objectives of the project have been provided a methodology, an implementing framework and a reporting system which countries can follow in meeting their future reporting obligations under the FCCC and for GEF enabling activities. The project builds upon the methodology development and application in the UNEP National Abatement Coasting Studies (UNEP, 1994a). The various elements provide countries with a road map for conducting climate change mitigation studies and submitting national reports as required by the FCCC. (au) 121 refs.

Methodological guidelines

International Nuclear Information System (INIS)

Halsnaes, K.; Callaway, J.M.; Meyer, H.J.

1999-01-01

The guideline document establishes a general overview of the main components of climate change mitigation assessment. This includes an outline of key economic concepts, scenario structure, common assumptions, modelling tools and country study assumptions. The guidelines are supported by Handbook Reports that contain more detailed specifications of calculation standards, input assumptions and available tools. The major objectives of the project have been provided a methodology, an implementing framework and a reporting system which countries can follow in meeting their future reporting obligations under the FCCC and for GEF enabling activities. The project builds upon the methodology development and application in the UNEP National Abatement Coasting Studies (UNEP, 1994a). The various elements provide countries with a road map for conducting climate change mitigation studies and submitting national reports as required by the FCCC. (au) 121 refs

Primary Spinal OPC Culture System from Adult Zebrafish to Study Oligodendrocyte Differentiation In Vitro

Directory of Open Access Journals (Sweden)

Volker Kroehne

2017-09-01

Full Text Available Endogenous oligodendrocyte progenitor cells (OPCs are a promising target to improve functional recovery after spinal cord injury (SCI by remyelinating denuded, and therefore vulnerable, axons. Demyelination is the result of a primary insult and secondary injury, leading to conduction blocks and long-term degeneration of the axons, which subsequently can lead to the loss of their neurons. In response to SCI, dormant OPCs can be activated and subsequently start to proliferate and differentiate into mature myelinating oligodendrocytes (OLs. Therefore, researchers strive to control OPC responses, and utilize small molecule screening approaches in order to identify mechanisms of OPC activation, proliferation, migration and differentiation. In zebrafish, OPCs remyelinate axons of the optic tract after lysophosphatidylcholine (LPC-induced demyelination back to full thickness myelin sheaths. In contrast to zebrafish, mammalian OPCs are highly vulnerable to excitotoxic stress, a cause of secondary injury, and remyelination remains insufficient. Generally, injury induced remyelination leads to shorter internodes and thinner myelin sheaths in mammals. In this study, we show that myelin sheaths are lost early after a complete spinal transection injury, but are re-established within 14 days after lesion. We introduce a novel, easy-to-use, inexpensive and highly reproducible OPC culture system based on dormant spinal OPCs from adult zebrafish that enables in vitro analysis. Zebrafish OPCs are robust, can easily be purified with high viability and taken into cell culture. This method enables to examine why zebrafish OPCs remyelinate better than their mammalian counterparts, identify cell intrinsic responses, which could lead to pro-proliferating or pro-differentiating strategies, and to test small molecule approaches. In this methodology paper, we show efficient isolation of OPCs from adult zebrafish spinal cord and describe culture conditions that enable

Beam geometry, alignment, and wavefront aberration effects on interferometric differential wavefront sensing

International Nuclear Information System (INIS)

Yu, Xiangzhi; Gillmer, S R; Ellis, J D

2015-01-01

Heterodyne interferometry is a widely accepted methodology with high resolution in many metrology applications. As a functionality enhancement, differential wavefront sensing (DWS) enables simultaneous measurement of displacement, pitch, and yaw using a displacement interferometry system and a single beam incident on a plane mirror target. The angular change is measured using a weighted phase average between symmetrically adjacent quadrant photodiode pairs. In this paper, we present an analytical model to predict the scaling of differential phase signals based on fundamental Gaussian beams. Several numerical models are presented to discuss the effects of physical beam parameters, detector size, system alignment errors, and beam wavefront aberrations on the DWS technique. The results of our modeling predict rotational scaling factors and a usable linear range. Furthermore, experimental results show the analytically predicted scaling factor is in good agreement with empirical calibration. Our three degree-of-freedom interferometer can achieve a resolution of 0.4 nm in displacement and 0.2 μrad in pitch and yaw simultaneously. (paper)

Practices to enable the geophysical research spectrum: from fundamentals to applications

Science.gov (United States)

Kang, S.; Cockett, R.; Heagy, L. J.; Oldenburg, D.

2016-12-01

In a geophysical survey, a source injects energy into the earth and a response is measured. These physical systems are governed by partial differential equations and their numerical solutions are obtained by discretizing the earth. Geophysical simulations and inversions are tools for understanding physical responses and constructing models of the subsurface given a finite amount of data. SimPEG (http://simpeg.xyz) is our effort to synthesize geophysical forward and inverse methodologies into a consistent framework. The primary focus of our initial development has been on the electromagnetics (EM) package, with recent extensions to magnetotelluric, direct current (DC), and induced polarization. Across these methods, and applied geophysics in general, we require tools to explore and build an understanding of the physics (behaviour of fields, fluxes), and work with data to produce models through reproducible inversions. If we consider DC or EM experiments, with the aim of understanding responses from subsurface conductors, we require resources that provide multiple "entry points" into the geophysical problem. To understand the physical responses and measured data, we must simulate the physical system and visualize electric fields, currents, and charges. Performing an inversion requires that many moving pieces be brought together: simulation, physics, linear algebra, data processing, optimization, etc. Each component must be trusted, accessible to interrogation and manipulation, and readily combined in order to enable investigation into inversion methodologies. To support such research, we not only require "entry points" into the software, but also extensibility to new situations. In our development of SimPEG, we have sought to use leading practices in software development with the aim of supporting and promoting collaborations across a spectrum of geophysical research: from fundamentals to applications. Designing software to enable this spectrum puts unique

New methodology developed for the differential scanning calorimetry analysis of polymeric matrixes incorporating phase change materials

International Nuclear Information System (INIS)

Barreneche, Camila; Solé, Aran; Miró, Laia; Martorell, Ingrid; Cabeza, Luisa F; Fernández, A Inés

2012-01-01

Nowadays, thermal comfort needs in buildings have led to an increase in energy consumption of the residential and service sectors. For this reason, thermal energy storage is shown as an alternative to achieve reduction of this high consumption. Phase change materials (PCM) have been studied to store energy due to their high storage capacity. A polymeric material capable of macroencapsulating PCM was developed by the authors of this paper. However, difficulties were found while measuring the thermal properties of these materials by differential scanning calorimetry (DSC). The polymeric matrix interferes in the detection of PCM properties by DSC. To remove this interfering effect, a new methodology which replaces the conventional empty crucible used as a reference in the DSC analysis by crucibles composed of the polymeric matrix was developed. Thus, a clear signal from the PCM is obtained by subtracting the new full crucible signal from the sample signal. (paper)

On matrix fractional differential equations

OpenAIRE

Adem Kılıçman; Wasan Ajeel Ahmood

2017-01-01

The aim of this article is to study the matrix fractional differential equations and to find the exact solution for system of matrix fractional differential equations in terms of Riemann–Liouville using Laplace transform method and convolution product to the Riemann–Liouville fractional of matrices. Also, we show the theorem of non-homogeneous matrix fractional partial differential equation with some illustrative examples to demonstrate the effectiveness of the new methodology. The main objec...

Evaluation of nasogastric tubes to enable differentiation between upper and lower gastrointestinal bleeding in unselected patients with melena.

Science.gov (United States)

Kessel, Boris; Olsha, Oded; Younis, Aurwa; Daskal, Yaakov; Granovsky, Emil; Alfici, Ricardo

2016-02-01

Gastrointestinal (GI) bleeding is a common surgical problem. The aim of this study was to evaluate how insertion of the nasogastric tube may enable differentiation between upper and lower GI bleeding in patients with melena. A retrospective study involving patients admitted to our surgery division with a melena was carried out between the years 2010 and 2012. A total of 386 patients were included in the study. Of these, 279 (72.2%) patients had negative nasogastric aspirate. The sensitivity of examination of nasogastric aspirate to establish the upper GI as the source of bleeding was only 28% and the negative predictive value of a negative nasogastric aspirate was less than 1%. Most patients who initially presented with melena and were found to have upper GI bleeding had a negative nasogastric aspirate. Insertion of a nasogastric tube does not affect the clinical decision to perform upper endoscopy and should not be routinely carried out.

The NLC Software Requirements Methodology

Energy Technology Data Exchange (ETDEWEB)

Shoaee, Hamid

2002-08-20

We describe the software requirements and development methodology developed for the NLC control system. Given the longevity of that project, and the likely geographical distribution of the collaborating engineers, the planned requirements management process is somewhat more formal than the norm in high energy physics projects. The short term goals of the requirements process are to accurately estimate costs, to decompose the problem, and to determine likely technologies. The long term goal is to enable a smooth transition from high level functional requirements to specific subsystem and component requirements for individual programmers, and to support distributed development. The methodology covers both ends of that life cycle. It covers both the analytical and documentary tools for software engineering, and project management support. This paper introduces the methodology, which is fully described in [1].

Sustainable Venture Capital Investments: An Enabler Investigation

Directory of Open Access Journals (Sweden)

Elena Antarciuc

2018-04-01

Full Text Available Investing in sustainable projects can help tackle the current sustainability challenges. Venture capital investments can contribute significantly to the growth of sustainable start-ups. Sustainable venture capital (SVC research is just emerging. This paper identifies enablers for sustainable venture capital investments in Saudi Arabia taking into account different stakeholders and firm’s tangible and intangible resources. Using perspectives from venture capital experts in Saudi Arabia and the grey-based Decision-Making Trial and Evaluation Laboratory (DEMATEL method, this study pinpoints the most critical enablers and investigates their causal and effect interconnections. The methodological process consists of reviewing the SVC literature and consulting the experts to identify the SVC enablers, creating a questionnaire, acquiring the answers from four experts, analyzing the data with grey-based DEMATEL and performing a sensitivity analysis. The government use of international standards, policies and regulations for sustainable investments, the commitment of the venture capitalists to sustainability and their deep understanding of sustainable business models are the most influential enablers. The paper concludes with implications for different actors, limitations and prospective directions for the sustainable venture capital research.

Active Dendrites and Differential Distribution of Calcium Channels Enable Functional Compartmentalization of Golgi Cells.

Science.gov (United States)

Rudolph, Stephanie; Hull, Court; Regehr, Wade G

2015-11-25

extent of inhibition depends on both spontaneous activity of GoCs and the excitatory synaptic input they receive. In this study, we find that different types of calcium channels are differentially distributed, with dendritic calcium channels being activated by somatic activity, boosting synaptic inputs and enabling bursting, and somatic calcium cannels promoting regular firing. We therefore challenge the current view that GoC dendrites are passive and identify the mechanisms that contribute to GoCs regulating the flow of sensory information in the cerebellar cortex. Copyright © 2015 the authors 0270-6474/15/3515492-13$15.00/0.

Differential equations problem solver

CERN Document Server

Arterburn, David R

2012-01-01

REA's Problem Solvers is a series of useful, practical, and informative study guides. Each title in the series is complete step-by-step solution guide. The Differential Equations Problem Solver enables students to solve difficult problems by showing them step-by-step solutions to Differential Equations problems. The Problem Solvers cover material ranging from the elementary to the advanced and make excellent review books and textbook companions. They're perfect for undergraduate and graduate studies.The Differential Equations Problem Solver is the perfect resource for any class, any exam, and

Application of different methodologies in the preparation of organic matrices for determination of trace elements by differential pulse anodic stripping voltammetry

International Nuclear Information System (INIS)

Sisti, Cristina

2001-01-01

The determination of trace elements in food samples is of great importance for the human health, considering the factors of essentiality and toxicity. On the other hand, the chemical analysis is largely affected for the steps of sample preparation; laboratory contamination of the sample and the reagents or still volatilization and losses of the elements. If these parameters are not controlled the achieved precision and accuracy could be low. In this work, the content of zinc, cadmium, lead and copper was determined in adults diet samples collected by duplicate portion technique and bovine liver, applying the differential pulse anodic stripping voltametry - (DP-ASV) technique. In the digestion of the matrices in acid medium, conventional methodologies were used, conductive heating in open recipients and equipment with microwaves source in open and closed vessels. The best procedure was the sample digestion by microwaves, in closed vessels and the other treatments made in controlled atmosphere with hood laminar-airflow class 100. The established methodology was validated with the use of a certified sample as reference (NIST - bovine liver 1577b). (author)

On production costs in vertical differentiation models

OpenAIRE

Dorothée Brécard

2009-01-01

In this paper, we analyse the effects of the introduction of a unit production cost beside a fixed cost of quality improvement in a duopoly model of vertical product differentiation. Thanks to an original methodology, we show that a low unit cost tends to reduce product differentiation and thus prices, whereas a high unit cost leads to widen product differentiation and to increase prices

Soft robot design methodology for `push-button' manufacturing

Science.gov (United States)

Paik, Jamie

2018-06-01

`Push-button' or fully automated manufacturing would enable the production of robots with zero intervention from human hands. Realizing this utopia requires a fundamental shift from a sequential (design-materials-manufacturing) to a concurrent design methodology.

Logistics service management; differentiating the logistics service

NARCIS (Netherlands)

Veeken, van der D.J.M.; Rutten, W.G.M.M.

1998-01-01

In this article a model is described, which enables differentiation of the logistics service that a company offers to its customers. Differentiating this service is essential for businesses with a large variation within their customer and/or products portfolio. The model consists of four phases:

A methodology to enable rapid evaluation of aviation environmental impacts and aircraft technologies

Science.gov (United States)

Becker, Keith Frederick

-processing, which does not capture physical interdependencies that may arise at the aircraft-level. The goal of the work that has been conducted here was the development of a methodology to develop surrogate fleet approaches that leverage the capability of physics-based aircraft models and the development of connectivity to fleet-level analysis tools to enable rapid evaluation of fuel burn and emissions metrics. Instead of requiring development of an individual physics-based model for each vehicle in the fleet, the surrogate fleet approaches seek to reduce the number of such models needed while still accurately capturing performance of the fleet. By reducing the number of models, both development time and execution time to generate fleet-level results may also be reduced. The initial steps leading to surrogate fleet formulation were a characterization of the commercial fleet into groups based on capability followed by the selection of a reference vehicle model and a reference set of operations for each group. Next, three potential surrogate fleet approaches were formulated. These approaches include the parametric correction factor approach, in which the results of a reference vehicle model are corrected to match the aggregate results of each group; the average replacement approach, in which a new vehicle model is developed to generate aggregate results of each group, and the best-in-class replacement approach, in which results for a reference vehicle are simply substituted for the entire group. Once candidate surrogate fleet approaches were developed, they were each applied to and evaluated over the set of reference operations. Then each approach was evaluated for their ability to model variations in operations. Finally, the ability of each surrogate fleet approach to capture implementation of different technology suites along with corresponding interdependencies between fuel burn and emissions was evaluated using the concept of a virtual fleet to simulate the technology response

Evaluating the enablers in solar power developments in the current scenario using fuzzy DEMATEL

DEFF Research Database (Denmark)

Luthra, Sunil; Govindan, Kannan; Kharb, Ravinder K.

2016-01-01

Determining solar power initiatives and developments for a country as large as India is difficult due to the involvement of different enablers. The decisions of these enablers will influence the formulation of strategies to encourage solar power development in India. The present research work...... critically analyzes Indian solar power developments to recognize and to evaluate key enablers that will encourage greater usage in Indias current scenario. A literature review that explores the Indian solar power sector is included, with a focus on need potential, and an examination of the key enablers....... This work identifies sixteen solar power enablers based on relevant literature and experts inputs. To evaluate and to categorize the recognized solar power development key enablers, a fuzzy Decision Making Trial and Evaluation Laboratory (DEMATEL) based methodology is utilized. The fuzzy DEMATEL approach...

A new methodology capable of characterizing most volatile and less volatile minor edible oils components in a single chromatographic run without solvents or reagents. Detection of new components.

Science.gov (United States)

Alberdi-Cedeño, Jon; Ibargoitia, María L; Cristillo, Giovanna; Sopelana, Patricia; Guillén, María D

2017-04-15

The possibilities offered by a new methodology to determine minor components in edible oils are described. This is based on immersion of a solid-phase microextraction fiber of PDMS/DVB into the oil matrix, followed by Gas Chromatography/Mass Spectrometry. It enables characterization and differentiation of edible oils in a simple way, without either solvents or sample modification. This methodology allows simultaneous identification and quantification of sterols, tocols, hydrocarbons of different natures, fatty acids, esters, monoglycerides, fatty amides, aldehydes, ketones, alcohols, epoxides, furans, pyrans and terpenic oxygenated derivatives. The broad information provided by this methodology is useful for different areas of interest such as nutritional value, oxidative stability, technological performance, quality, processing, safety and even the prevention of fraudulent practices. Furthermore, for the first time, certain fatty amides, gamma- and delta-lactones of high molecular weight, and other aromatic compounds such as some esters derived from cinnamic acid have been detected in edible oils. Copyright © 2016 Elsevier Ltd. All rights reserved.

Two-photon microscopy for non-invasive, quantitative monitoring of stem cell differentiation.

Directory of Open Access Journals (Sweden)

William L Rice

Full Text Available BACKGROUND: The engineering of functional tissues is a complex multi-stage process, the success of which depends on the careful control of culture conditions and ultimately tissue maturation. To enable the efficient optimization of tissue development protocols, techniques suitable for monitoring the effects of added stimuli and induced tissue changes are needed. METHODOLOGY/PRINCIPAL FINDINGS: Here, we present the quantitative use of two-photon excited fluorescence (TPEF and second harmonic generation (SHG as a noninvasive means to monitor the differentiation of human mesenchymal stem cells (hMSCs using entirely endogenous sources of contrast. We demonstrate that the individual fluorescence contribution from the intrinsic cellular fluorophores NAD(PH, flavoproteins and lipofuscin can be extracted from TPEF images and monitored dynamically from the same cell population over time. Using the redox ratio, calculated from the contributions of NAD(PH and flavoproteins, we identify distinct patterns in the evolution of the metabolic activity of hMSCs maintained in either propagation, osteogenic or adipogenic differentiation media. The differentiation of these cells is mirrored by changes in cell morphology apparent in high resolution TPEF images and by the detection of collagen production via SHG imaging. Finally, we find dramatic increases in lipofuscin levels in hMSCs maintained at 20% oxygen vs. those in 5% oxygen, establishing the use of this chromophore as a potential biomarker for oxidative stress. CONCLUSIONS/SIGNIFICANCE: In this study we demonstrate that it is possible to monitor the metabolic activity, morphology, ECM production and oxidative stress of hMSCs in a non-invasive manner. This is accomplished using generally available multiphoton microscopy equipment and simple data analysis techniques, such that the method can widely adopted by laboratories with a diversity of comparable equipment. This method therefore represents a powerful tool

Knowledge Management in Higher Education Institutions: Enablers and Barriers in Mauritius

Science.gov (United States)

Veer Ramjeawon, Poonam; Rowley, Jennifer

2017-01-01

Purpose: The purpose of this study is to contribute to research on knowledge management in higher education institutions (HEIs), by studying the enablers and barriers to knowledge management in a country with a developing higher education sector, Mauritius. Design/methodology/approach: Semi-structured interviews were conducted with senior staff in…

Knowledge Management Practices and Enablers in Public Universities: A Gap Analysis

Science.gov (United States)

Ramachandran, Sharimllah Devi; Chong, Siong-Choy; Wong, Kuan-Yew

2013-01-01

Purpose: The purpose of this paper is to examine the gap between knowledge management (KM) practices and key strategic enablers in public universities. For this purpose, a 57-item survey on two dimensions--"use" and "importance"--was used as the instrument for this study. Design/methodology/approach: The questionnaire was…

Methodology and Psychological Knowledge Issues in the Procedural Differentiation Sphere and the Evidence Doctrine: Problems of Theory and Legislation

Directory of Open Access Journals (Sweden)

Sergey S. Tsyganenko

2016-09-01

Full Text Available The paper is devoted to actual problems of the theory and legislation of the modern criminal trial based on provisions of differentiation of a procedural form. Its basic provisions belong also to questions of evidentiary activity. Characteristics of knowledge on criminal case will claim the psychological knowledge and categories included in the mechanism of legal regulation of criminal procedure activity. Key role is allocated for the principle of freedom of an assessment of proofs. In this case the assessment of proofs is carried out on internal belief. Historically it is connected with institute of jury trial and competitiveness. Both the principle of freedom of assessment of proofs, and many other provisions of the procedural theory and the legislation need interpretation and an explanation with use of modern psychological knowledge. At the same time in modern Russian criminal procedure as well as in foreign types of criminal trial the reduced summary or special procedural jurisdictions are actively applied. It means formation of the differentiated model of criminal legal proceedings at which there is a summary process. Simplification of an order of proof on a criminal case through a wide range of exceptions is characteristic of them that constitutes a theoretical and practical problem. At their decision it is necessary to use a modern arsenal of methodology and the theory, including new achievements in the field of psychological knowledge.

Implementation of Prognostic Methodologies to Cryogenic Propellant Loading Test-bed

Data.gov (United States)

National Aeronautics and Space Administration — Prognostics methodologies determine the health state of a system and predict the end of life and remaining useful life. This information enables operators to take...

Enabling high performance computational science through combinatorial algorithms

International Nuclear Information System (INIS)

Boman, Erik G; Bozdag, Doruk; Catalyurek, Umit V; Devine, Karen D; Gebremedhin, Assefaw H; Hovland, Paul D; Pothen, Alex; Strout, Michelle Mills

2007-01-01

The Combinatorial Scientific Computing and Petascale Simulations (CSCAPES) Institute is developing algorithms and software for combinatorial problems that play an enabling role in scientific and engineering computations. Discrete algorithms will be increasingly critical for achieving high performance for irregular problems on petascale architectures. This paper describes recent contributions by researchers at the CSCAPES Institute in the areas of load balancing, parallel graph coloring, performance improvement, and parallel automatic differentiation

Enabling high performance computational science through combinatorial algorithms

Energy Technology Data Exchange (ETDEWEB)

Boman, Erik G [Discrete Algorithms and Math Department, Sandia National Laboratories (United States); Bozdag, Doruk [Biomedical Informatics, and Electrical and Computer Engineering, Ohio State University (United States); Catalyurek, Umit V [Biomedical Informatics, and Electrical and Computer Engineering, Ohio State University (United States); Devine, Karen D [Discrete Algorithms and Math Department, Sandia National Laboratories (United States); Gebremedhin, Assefaw H [Computer Science and Center for Computational Science, Old Dominion University (United States); Hovland, Paul D [Mathematics and Computer Science Division, Argonne National Laboratory (United States); Pothen, Alex [Computer Science and Center for Computational Science, Old Dominion University (United States); Strout, Michelle Mills [Computer Science, Colorado State University (United States)

2007-07-15

The Combinatorial Scientific Computing and Petascale Simulations (CSCAPES) Institute is developing algorithms and software for combinatorial problems that play an enabling role in scientific and engineering computations. Discrete algorithms will be increasingly critical for achieving high performance for irregular problems on petascale architectures. This paper describes recent contributions by researchers at the CSCAPES Institute in the areas of load balancing, parallel graph coloring, performance improvement, and parallel automatic differentiation.

Creating an enabling environment for WR&R implementation.

Science.gov (United States)

Stathatou, P-M; Kampragou, E; Grigoropoulou, H; Assimacopoulos, D; Karavitis, C; Gironás, J

2017-09-01

Reclaimed water is receiving growing attention worldwide as an effective solution for alleviating the growing water scarcity in many areas. Despite the various benefits associated with reclaimed water, water recycling and reuse (WR&R) practices are not widely applied around the world. This is mostly due to complex and inadequate local legal and institutional frameworks and socio-economic structures, which pose barriers to wider WR&R implementation. An integrated approach is therefore needed while planning the implementation of WR&R schemes, considering all the potential barriers, and aiming to develop favourable conditions for enhancing reclaimed water use. This paper proposes a comprehensive methodology supporting the development of an enabling environment for WR&R implementation. The political, economic, social, technical, legal and institutional factors that may influence positively (drivers) or negatively (barriers) WR&R implementation in the regional water systems are identified, through the mapping of local stakeholder perceptions. The identified barriers are further analysed, following a Cross-Impact/System analysis, to recognize the most significant barriers inhibiting system transition, and to prioritize the enabling instruments and arrangements that are needed to boost WR&R implementation. The proposed methodology was applied in the Copiapó River Basin in Chile, which faces severe water scarcity. Through the analysis, it was observed that barriers outweigh drivers for the implementation of WR&R schemes in the Copiapó River Basin, while the key barriers which could be useful for policy formulation towards an enabling environment in the area concern the unclear legal framework regarding the ownership of treated wastewater, the lack of environmental policies focusing on pollution control, the limited integration of reclaimed water use in current land use and development policies, the limited public awareness on WR&R, and the limited availability of

The Behavior of Procurement Process as Described by Using System Dynamics Methodology

OpenAIRE

Mohd Yusoff, Mohd Izhan

2018-01-01

System dynamics methodology has been used in many fields of study which include supply chain, project management and performance, and procurement process. The said methodology enables the researchers to identify and study the impact of the variables or factors on the outcome of the model they developed. In this paper, we showed the use of system dynamics methodology in studying the behavior of procurement process that is totally different from those mentioned in previous studies. By using a t...

Blue space geographies: Enabling health in place.

Science.gov (United States)

Foley, Ronan; Kistemann, Thomas

2015-09-01

Drawing from research on therapeutic landscapes and relationships between environment, health and wellbeing, we propose the idea of 'healthy blue space' as an important new development Complementing research on healthy green space, blue space is defined as; 'health-enabling places and spaces, where water is at the centre of a range of environments with identifiable potential for the promotion of human wellbeing'. Using theoretical ideas from emotional and relational geographies and critical understandings of salutogenesis, the value of blue space to health and wellbeing is recognised and evaluated. Six individual papers from five different countries consider how health can be enabled in mixed blue space settings. Four sub-themes; embodiment, inter-subjectivity, activity and meaning, document multiple experiences within a range of healthy blue spaces. Finally, we suggest a considerable research agenda - theoretical, methodological and applied - for future work within different forms of blue space. All are suggested as having public health policy relevance in social and public space. Copyright © 2015 Elsevier Ltd. All rights reserved.

A Continuous Formulation for Logical Decisions in Differential Algebraic Systems using Mathematical Programs with Complementarity Constraints

Directory of Open Access Journals (Sweden)

Kody M. Powell

2016-03-01

Full Text Available This work presents a methodology to represent logical decisions in differential algebraic equation simulation and constrained optimization problems using a set of continuous algebraic equations. The formulations may be used when state variables trigger a change in process dynamics, and introduces a pseudo-binary decision variable, which is continuous, but should only have valid solutions at values of either zero or one within a finite time horizon. This formulation enables dynamic optimization problems with logical disjunctions to be solved by simultaneous solution methods without using methods such as mixed integer programming. Several case studies are given to illustrate the value of this methodology including nonlinear model predictive control of a chemical reactor using a surge tank with overflow to buffer disturbances in feed flow rate. Although this work contains novel methodologies for solving dynamic algebraic equation (DAE constrained problems where the system may experience an abrupt change in dynamics that may otherwise require a conditional statement, there remain substantial limitations to this methodology, including a limited domain where problems may converge and the possibility for ill-conditioning. Although the problems presented use only continuous algebraic equations, the formulation has inherent non-smoothness. Hence, these problems must be solved with care and only in select circumstances, such as in simulation or situations when the solution is expected to be near the solver’s initial point.

Barriers and enablers to physical activity participation in patients with COPD: a systematic review.

Science.gov (United States)

Thorpe, Olivia; Johnston, Kylie; Kumar, Saravana

2012-01-01

Physical activity (PA) has been shown to improve symptoms in people with chronic obstructive pulmonary disease (COPD). Despite the high health and financial costs, the uptake of management strategies, particularly participation in PA and pulmonary rehabilitation (PR), are low. The review objective here was to identify potential barriers and enablers, which people with COPD report being associated with their participation in PA programs, including PR. A systematic search was undertaken to identify studies (published Jan 2000 to Aug 2011) reporting any barriers and enablers experienced by people with COPD regarding participation in PA and PR. Methodological quality of the studies was appraised using McMaster critical appraisal tools. A narrative summary of findings was undertaken reporting on individual study characteristics, country of origin, participants, and potential barriers and enablers. Eleven studies (8 qualitative and 3 quantitative) met the inclusion criteria for this systematic review. Several methodological issues (small sampling, poor description of data collection and analysis, issues with generalizability of the research findings) were common among included studies. Barriers identified included changing health status, personal issues, lack of support, external factors, ongoing smoking, and program-specific barriers. Enablers identified included social support, professional support, personal drivers, personal benefit, control of condition, specific goals, and program-specific enablers. The findings from this review may assist health professionals, patients, care givers and the wider community to develop effective strategies to promote participation in PA and PR among people with COPD.

Design methodology of Dutch banknotes

Science.gov (United States)

de Heij, Hans A. M.

2000-04-01

Since the introduction of a design methodology for Dutch banknotes, the quality of Dutch paper currency has improved in more than one way. The methodology is question provides for (i) a design policy, which helps fix clear objectives; (ii) design management, to ensure a smooth cooperation between the graphic designer, printer, papermaker an central bank, (iii) a program of requirements, a banknote development guideline for all parties involved. This systematic approach enables an objective selection of design proposals, including security features. Furthermore, the project manager obtains regular feedback from the public by conducting market surveys. Each new design of a Netherlands Guilder banknote issued by the Nederlandsche Bank of the past 50 years has been an improvement on its predecessor in terms of value recognition, security and durability.

On matrix fractional differential equations

Directory of Open Access Journals (Sweden)

Adem Kılıçman

2017-01-01

Full Text Available The aim of this article is to study the matrix fractional differential equations and to find the exact solution for system of matrix fractional differential equations in terms of Riemann–Liouville using Laplace transform method and convolution product to the Riemann–Liouville fractional of matrices. Also, we show the theorem of non-homogeneous matrix fractional partial differential equation with some illustrative examples to demonstrate the effectiveness of the new methodology. The main objective of this article is to discuss the Laplace transform method based on operational matrices of fractional derivatives for solving several kinds of linear fractional differential equations. Moreover, we present the operational matrices of fractional derivatives with Laplace transform in many applications of various engineering systems as control system. We present the analytical technique for solving fractional-order, multi-term fractional differential equation. In other words, we propose an efficient algorithm for solving fractional matrix equation.

Solving Differential Equations Analytically. Elementary Differential Equations. Modules and Monographs in Undergraduate Mathematics and Its Applications Project. UMAP Unit 335.

Science.gov (United States)

Goldston, J. W.

This unit introduces analytic solutions of ordinary differential equations. The objective is to enable the student to decide whether a given function solves a given differential equation. Examples of problems from biology and chemistry are covered. Problem sets, quizzes, and a model exam are included, and answers to all items are provided. The…

METHODOLOGICAL ELEMENTS OF SITUATIONAL ANALYSIS

Directory of Open Access Journals (Sweden)

Tetyana KOVALCHUK

2016-07-01

Full Text Available The article deals with the investigation of theoretical and methodological principles of situational analysis. The necessity of situational analysis is proved in modern conditions. The notion “situational analysis” is determined. We have concluded that situational analysis is a continuous system study which purpose is to identify dangerous situation signs, to evaluate comprehensively such signs influenced by a system of objective and subjective factors, to search for motivated targeted actions used to eliminate adverse effects of the exposure of the system to the situation now and in the future and to develop the managerial actions needed to bring the system back to norm. It is developed a methodological approach to the situational analysis, its goal is substantiated, proved the expediency of diagnostic, evaluative and searching functions in the process of situational analysis. The basic methodological elements of the situational analysis are grounded. The substantiation of the principal methodological elements of system analysis will enable the analyst to develop adaptive methods able to take into account the peculiar features of a unique object which is a situation that has emerged in a complex system, to diagnose such situation and subject it to system and in-depth analysis, to identify risks opportunities, to make timely management decisions as required by a particular period.

Differential discriminator

International Nuclear Information System (INIS)

Dukhanov, V.I.; Mazurov, I.B.

1981-01-01

A principal flowsheet of a differential discriminator intended for operation in a spectrometric circuit with statistical time distribution of pulses is described. The differential discriminator includes four integrated discriminators and a channel of piled-up signal rejection. The presence of the rejection channel enables the discriminator to operate effectively at loads of 14x10 3 pulse/s. The temperature instability of the discrimination thresholds equals 250 μV/ 0 C. The discrimination level changes within 0.1-5 V, the level shift constitutes 0.5% for the filling ratio of 1:10. The rejection coefficient is not less than 90%. Alpha spectrum of the 228 Th source is presented to evaluate the discriminator operation with the rejector. The rejector provides 50 ns time resolution

Males in Enabling: Painting a portrait through narrative

Directory of Open Access Journals (Sweden)

Frank Armstrong

2018-02-01

Full Text Available The number of males entering higher education via an enabling pathway is slowly increasing; yet, males still battle with the anti-intellectual attitude that is prevalent in regional areas of Australia. Previous research undertaken by the authors began exploring the factors that inhibited or enhanced the male experience within an enabling course. This paper expands upon this research with a deeper focus on the male experience through personalised accounts derived from individual interviews. Using qualitative methodology and narrative inquiry, the findings provide a deeper understanding of the issues that males of different ages face when creating a new identity as a university student. This paper shares insights into what motivated the male students to enter university via an enabling pathway; the actual personal experiences both positive and negative during this time; and the effect that this commitment to study had on them personally and the people around them. The lens of transformative theory underpins this research through exploring frames of reference that align with the students’ experiences. Portraiture prose shares the individual stories which are analysed and the key findings extrapolated.

Methodology of theory of stage-by-stage long-term preparation of sportsmen in single combats

Directory of Open Access Journals (Sweden)

Arziutov G.

2010-04-01

Full Text Available Results over of researches are brought on methodology of theory of stage-by-stage preparation of sportsmen in single combats. The structuralness of theory lies in possibility simple verifications of its substantive provisions, principles and laws. Development of methodology enables to begin creation of map of trainer on the stages of long-term preparation. Laws, conformities to law, principles and rules, must be collected in a map. A map enables the trainers of reserve sport to use its content during all stages of preparation of sportsman.

Competitive intelligence as an enabler for firm competitiveness: An overview

Directory of Open Access Journals (Sweden)

Alexander Maune

2014-06-01

Full Text Available The purpose of this article is to provide an overview, from literature, about how competitive intelligence can be an enabler towards a firm’s competitiveness. This overview is done under the background of intense global competition that firms are currently experiencing. This paper used a qualitative content analysis as a data collection methodology on all identified journal articles on competitive intelligence and firm competitiveness. To identify relevant literature, academic databases and search engines were used. Moreover, a review of references in related studies led to more relevant sources, the references of which were further reviewed and analysed. To ensure reliability and trustworthiness, peer-reviewed journal articles and triangulation were used. The paper found that competitive intelligence is an important enabler of firm competitiveness. The findings from this paper will assist business managers to understand and improve their outlook of competitive intelligence as an enabler of firm competitiveness and will be of great academic value.

Methodological remarks on contraction theory

DEFF Research Database (Denmark)

Jouffroy, Jerome; Slotine, Jean-Jacques E.

Because contraction analysis stems from a differential and incremental framework, the nature and methodology of contraction-based proofs are significantly different from those of their Lyapunov-based counterparts. This paper specifically studies this issue, and illustrates it by revisiting some c...... classical examples traditionally addressed using Lyapunov theory. Even in these cases, contraction tools can often yield significantly simplified analysis. The examples include adaptive control, robotics, and a proof of convergence of the deterministic Extended Kalman Filter....

Survey of Dynamic PSA Methodologies

International Nuclear Information System (INIS)

Lee, Hansul; Kim, Hyeonmin; Heo, Gyunyoung; Kim, Taewan

2015-01-01

Event Tree(ET)/Fault Tree(FT) are significant methodology in Probabilistic Safety Assessment(PSA) for Nuclear Power Plants(NPPs). ET/FT methodology has the advantage for users to be able to easily learn and model. It enables better communication between engineers engaged in the same field. However, conventional methodologies are difficult to cope with the dynamic behavior (e.g. operation mode changes or sequence-dependent failure) and integrated situation of mechanical failure and human errors. Meanwhile, new possibilities are coming for the improved PSA by virtue of the dramatic development on digital hardware, software, information technology, and data analysis.. More specifically, the computing environment has been greatly improved with being compared to the past, so we are able to conduct risk analysis with the large amount of data actually available. One method which can take the technological advantages aforementioned should be the dynamic PSA such that conventional ET/FT can have time- and condition-dependent behaviors in accident scenarios. In this paper, we investigated the various enabling techniques for the dynamic PSA. Even though its history and academic achievement was great, it seems less interesting from industrial and regulatory viewpoint. Authors expect this can contribute to better understanding of dynamic PSA in terms of algorithm, practice, and applicability. In paper, the overview for the dynamic PSA was conducted. Most of methodologies share similar concepts. Among them, DDET seems a backbone for most of methodologies since it can be applied to large problems. The common characteristics sharing the concept of DDET are as follows: • Both deterministic and stochastic approaches • Improves the identification of PSA success criteria • Helps to limit detrimental effects of sequence binning (normally adopted in PSA) • Helps to avoid defining non-optimal success criteria that may distort the risk • Framework for comprehensively considering

Survey of Dynamic PSA Methodologies

Energy Technology Data Exchange (ETDEWEB)

Lee, Hansul; Kim, Hyeonmin; Heo, Gyunyoung [Kyung Hee University, Yongin (Korea, Republic of); Kim, Taewan [KEPCO International Nuclear Graduate School, Ulsan (Korea, Republic of)

2015-05-15

Event Tree(ET)/Fault Tree(FT) are significant methodology in Probabilistic Safety Assessment(PSA) for Nuclear Power Plants(NPPs). ET/FT methodology has the advantage for users to be able to easily learn and model. It enables better communication between engineers engaged in the same field. However, conventional methodologies are difficult to cope with the dynamic behavior (e.g. operation mode changes or sequence-dependent failure) and integrated situation of mechanical failure and human errors. Meanwhile, new possibilities are coming for the improved PSA by virtue of the dramatic development on digital hardware, software, information technology, and data analysis.. More specifically, the computing environment has been greatly improved with being compared to the past, so we are able to conduct risk analysis with the large amount of data actually available. One method which can take the technological advantages aforementioned should be the dynamic PSA such that conventional ET/FT can have time- and condition-dependent behaviors in accident scenarios. In this paper, we investigated the various enabling techniques for the dynamic PSA. Even though its history and academic achievement was great, it seems less interesting from industrial and regulatory viewpoint. Authors expect this can contribute to better understanding of dynamic PSA in terms of algorithm, practice, and applicability. In paper, the overview for the dynamic PSA was conducted. Most of methodologies share similar concepts. Among them, DDET seems a backbone for most of methodologies since it can be applied to large problems. The common characteristics sharing the concept of DDET are as follows: • Both deterministic and stochastic approaches • Improves the identification of PSA success criteria • Helps to limit detrimental effects of sequence binning (normally adopted in PSA) • Helps to avoid defining non-optimal success criteria that may distort the risk • Framework for comprehensively considering

Globalization and Its Influence on Comparative Education Methodology

Science.gov (United States)

Chigisheva, Oksana

2015-01-01

The article is devoted to the research of the methodological changes that occur in the field of comparative education as a result of globalization. A deep analysis of the globalization phenomenon is undertaken with a special focus on the differentiation of globalization, internationalisation, regionalisation and integration. UNESCO's role in the…

Differential potentiometric titration: development of a methodology for determining the point of zero charge of metal (hydr)oxides by one titration curve.

Science.gov (United States)

Bourikas, Kyriakos; Kordulis, Christos; Lycourghiotis, Alexis

2005-06-01

A new methodology is presented, called differential potentiometric titration (DPT), which allows the determination of the point of zero charge (pzc) of metal (hydr)oxides using only one potentiometric curve. By performing extensive simulations of potentiometric titrations for various model (hydr)oxides, we found that an inflection point in a H+(cons,surf) versus pH potentiometric curve (H+(cons,surf): hydrogen ions consumed on the surface of the (hydr)oxide) and a peak in the corresponding differential curve, dH+(cons,surf)/dpH versus pH, appear at a pH equal to the pzc assumed for a model (hydr)oxide. This distinguishable peak appears at the same position irrespective of the surface ionization and the interfacial model adopted as well as the assumed ionic strength. It was found that the aforementioned peak also appears in the high-resolution differential potentiometric curves experimentally determined for four oxides (SiO2, TiO2, gamma-Al2O3, and MgO) that are widely used in various environmental and other technological applications. The application of DPT to the above-mentioned oxides provided practically the same pzc values as the corresponding ones achieved by using four different techniques as well as the corresponding isoelectric point (iep) values determined by microelectrophoresis. Differences between the pzc and iep values determined using various techniques in the case of MgO were attributed to the increasing dissolution of this oxide as pH decreases and the adsorption of cations (Mg2+, Na+) on the MgO/electrolytic solution interface.

Solution of some types of differential equations: operational calculus and inverse differential operators.

Science.gov (United States)

Zhukovsky, K

2014-01-01

We present a general method of operational nature to analyze and obtain solutions for a variety of equations of mathematical physics and related mathematical problems. We construct inverse differential operators and produce operational identities, involving inverse derivatives and families of generalised orthogonal polynomials, such as Hermite and Laguerre polynomial families. We develop the methodology of inverse and exponential operators, employing them for the study of partial differential equations. Advantages of the operational technique, combined with the use of integral transforms, generating functions with exponentials and their integrals, for solving a wide class of partial derivative equations, related to heat, wave, and transport problems, are demonstrated.

Differential sensor in front photopyroelectric technique: II. Experimental

International Nuclear Information System (INIS)

Ivanov, R; Moreno, I; Araujo-Andrade, C; MarIn, E; Cruz-Orea, A; Pichardo-Molina, J L

2009-01-01

We describe the differential cell design and the experimental (optical and electronic) setup for the differential front photopyroelectric technique, whose theory has been developed in the first part of this paper (Ivanov et al 2008 J. Phys. D: Appl. Phys. 41 085106). We will show first how the direct (non-differential) front photopyroelectric theory described in our previous paper reproduces well the experimental results. The usefulness of the differential technique is demonstrated by means of experimental measurements of the thermal effusivity in binary ethanol-water and glycerol-water mixtures, based on a theoretical methodology that simplifies the measurement procedure and diminishes the experimental uncertainty.

Differential sensor in front photopyroelectric technique: II. Experimental

Energy Technology Data Exchange (ETDEWEB)

Ivanov, R; Moreno, I; Araujo-Andrade, C [Facultad de Fisica, Universidad Autonoma de Zacatecas, Calz. Solidaridad Esquina Paseo de la Bufa s/n, CP 98060, Zacatecas, Zac. (Mexico); MarIn, E [Centro de Investigacion en Ciencia Aplicada y TecnologIa Avanzada-Instituto Politecnico Nacional, Legaria 694, Col. Irrigacion, CP 11500, Mexico D.F. (Mexico); Cruz-Orea, A [Departamento de Fisica, CINVESTAV-IPN, Av. IPN No. 2508, Col. San Pedro Zacatenco, CP 07360, Mexico D.F. (Mexico); Pichardo-Molina, J L, E-mail: rumen@fisica.uaz.edu.m [Centro de Investigaciones en Optica, Loma del Bosque 115, Loma del Campestre, CP 37150, Leon, Guanajuato (Mexico)

2009-06-21

We describe the differential cell design and the experimental (optical and electronic) setup for the differential front photopyroelectric technique, whose theory has been developed in the first part of this paper (Ivanov et al 2008 J. Phys. D: Appl. Phys. 41 085106). We will show first how the direct (non-differential) front photopyroelectric theory described in our previous paper reproduces well the experimental results. The usefulness of the differential technique is demonstrated by means of experimental measurements of the thermal effusivity in binary ethanol-water and glycerol-water mixtures, based on a theoretical methodology that simplifies the measurement procedure and diminishes the experimental uncertainty.

Algorithms For Integrating Nonlinear Differential Equations

Science.gov (United States)

Freed, A. D.; Walker, K. P.

1994-01-01

Improved algorithms developed for use in numerical integration of systems of nonhomogenous, nonlinear, first-order, ordinary differential equations. In comparison with integration algorithms, these algorithms offer greater stability and accuracy. Several asymptotically correct, thereby enabling retention of stability and accuracy when large increments of independent variable used. Accuracies attainable demonstrated by applying them to systems of nonlinear, first-order, differential equations that arise in study of viscoplastic behavior, spread of acquired immune-deficiency syndrome (AIDS) virus and predator/prey populations.

Topics in numerical partial differential equations and scientific computing

CERN Document Server

2016-01-01

Numerical partial differential equations (PDEs) are an important part of numerical simulation, the third component of the modern methodology for science and engineering, besides the traditional theory and experiment. This volume contains papers that originated with the collaborative research of the teams that participated in the IMA Workshop for Women in Applied Mathematics: Numerical Partial Differential Equations and Scientific Computing in August 2014.

High Weak Order Methods for Stochastic Differential Equations Based on Modified Equations

KAUST Repository

Abdulle, Assyr

2012-01-01

© 2012 Society for Industrial and Applied Mathematics. Inspired by recent advances in the theory of modified differential equations, we propose a new methodology for constructing numerical integrators with high weak order for the time integration of stochastic differential equations. This approach is illustrated with the constructions of new methods of weak order two, in particular, semi-implicit integrators well suited for stiff (meansquare stable) stochastic problems, and implicit integrators that exactly conserve all quadratic first integrals of a stochastic dynamical system. Numerical examples confirm the theoretical results and show the versatility of our methodology.

Essential methodological considerations when using grounded theory.

Science.gov (United States)

Achora, Susan; Matua, Gerald Amandu

2016-07-01

To suggest important methodological considerations when using grounded theory. A research method widely used in nursing research is grounded theory, at the centre of which is theory construction. However, researchers still struggle with some of its methodological issues. Although grounded theory is widely used to study and explain issues in nursing practice, many researchers are still failing to adhere to its rigorous standards. Researchers should articulate the focus of their investigations - the substantive area of interest as well as the focal population. This should be followed by a succinct explanation of the strategies used to collect and analyse data, supported by clear coding processes. Finally, the resolution of the core issues, including the core category and related categories, should be explained to advance readers' understanding. Researchers should endeavour to understand the tenets of grounded theory. This enables 'neophytes' in particular to make methodological decisions that will improve their studies' rigour and fit with grounded theory. This paper complements the current dialogue on improving the understanding of grounded theory methodology in nursing research. The paper also suggests important procedural decisions researchers need to make to preserve their studies' scientific merit and fit with grounded theory.

High-Throughput Screening Enhances Kidney Organoid Differentiation from Human Pluripotent Stem Cells and Enables Automated Multidimensional Phenotyping.

Science.gov (United States)

Czerniecki, Stefan M; Cruz, Nelly M; Harder, Jennifer L; Menon, Rajasree; Annis, James; Otto, Edgar A; Gulieva, Ramila E; Islas, Laura V; Kim, Yong Kyun; Tran, Linh M; Martins, Timothy J; Pippin, Jeffrey W; Fu, Hongxia; Kretzler, Matthias; Shankland, Stuart J; Himmelfarb, Jonathan; Moon, Randall T; Paragas, Neal; Freedman, Benjamin S

2018-05-15

Organoids derived from human pluripotent stem cells are a potentially powerful tool for high-throughput screening (HTS), but the complexity of organoid cultures poses a significant challenge for miniaturization and automation. Here, we present a fully automated, HTS-compatible platform for enhanced differentiation and phenotyping of human kidney organoids. The entire 21-day protocol, from plating to differentiation to analysis, can be performed automatically by liquid-handling robots, or alternatively by manual pipetting. High-content imaging analysis reveals both dose-dependent and threshold effects during organoid differentiation. Immunofluorescence and single-cell RNA sequencing identify previously undetected parietal, interstitial, and partially differentiated compartments within organoids and define conditions that greatly expand the vascular endothelium. Chemical modulation of toxicity and disease phenotypes can be quantified for safety and efficacy prediction. Screening in gene-edited organoids in this system reveals an unexpected role for myosin in polycystic kidney disease. Organoids in HTS formats thus establish an attractive platform for multidimensional phenotypic screening. Copyright © 2018 Elsevier Inc. All rights reserved.

A Quality-Driven Methodology for Information Systems Integration

Directory of Open Access Journals (Sweden)

Iyad Zikra

2017-10-01

Full Text Available Information systems integration is an essential instrument for organizations to attain advantage in today’s growing and fast changing business and technology landscapes. Integration solutions generate added value by combining the functionality and services of heterogeneous and diverse systems. Existing integration environments tend to rely heavily on technical, platform-dependent skills. Consequently, the solutions that they enable are not optimally aligned with the envisioned business goals of the organization. Furthermore, the gap between the goals and the solutions complicates the task of evaluating the quality of integration solutions. To address these challenges, we propose a quality-driven, model-driven methodology for designing and developing integration solutions. The methodology spans organizational and systems design details, providing a holistic view of the integration solution and its underlying business goals. A multi-view meta-model provides the basis for the integration design. Quality factors that affect various aspects of the integration solution guide and inform the progress of the methodology. An example business case is presented to demonstrate the application of the methodology.

A methodology for digital soil mapping in poorly-accessible areas

NARCIS (Netherlands)

Cambule, A.; Rossiter, D.G.; Stoorvogel, J.J.

2013-01-01

Effective soil management requires knowledge of the spatial patterns of soil variation within the landscape to enable wise land use decisions. This is typically obtained through time-consuming and costly surveys. The aim of this study was to develop a cost-efficient methodology for digital soil

An integrated methodology for the dynamic performance and reliability evaluation of fault-tolerant systems

International Nuclear Information System (INIS)

Dominguez-Garcia, Alejandro D.; Kassakian, John G.; Schindall, Joel E.; Zinchuk, Jeffrey J.

2008-01-01

We propose an integrated methodology for the reliability and dynamic performance analysis of fault-tolerant systems. This methodology uses a behavioral model of the system dynamics, similar to the ones used by control engineers to design the control system, but also incorporates artifacts to model the failure behavior of each component. These artifacts include component failure modes (and associated failure rates) and how those failure modes affect the dynamic behavior of the component. The methodology bases the system evaluation on the analysis of the dynamics of the different configurations the system can reach after component failures occur. For each of the possible system configurations, a performance evaluation of its dynamic behavior is carried out to check whether its properties, e.g., accuracy, overshoot, or settling time, which are called performance metrics, meet system requirements. Markov chains are used to model the stochastic process associated with the different configurations that a system can adopt when failures occur. This methodology not only enables an integrated framework for evaluating dynamic performance and reliability of fault-tolerant systems, but also enables a method for guiding the system design process, and further optimization. To illustrate the methodology, we present a case-study of a lateral-directional flight control system for a fighter aircraft

An optimization methodology for identifying robust process integration investments under uncertainty

Energy Technology Data Exchange (ETDEWEB)

Svensson, Elin; Berntsson, Thore [Department of Energy and Environment, Division of Heat and Power Technology, Chalmers University of Technology, SE-412 96 Goeteborg (Sweden); Stroemberg, Ann-Brith [Fraunhofer-Chalmers Research Centre for Industrial Mathematics, Chalmers Science Park, SE-412 88 Gothenburg (Sweden); Patriksson, Michael [Department of Mathematical Sciences, Chalmers University of Technology and Department of Mathematical Sciences, University of Gothenburg, SE-412 96 Goeteborg (Sweden)

2009-02-15

Uncertainties in future energy prices and policies strongly affect decisions on investments in process integration measures in industry. In this paper, we present a five-step methodology for the identification of robust investment alternatives incorporating explicitly such uncertainties in the optimization model. Methods for optimization under uncertainty (or, stochastic programming) are thus combined with a deep understanding of process integration and process technology in order to achieve a framework for decision-making concerning the investment planning of process integration measures under uncertainty. The proposed methodology enables the optimization of investments in energy efficiency with respect to their net present value or an environmental objective. In particular, as a result of the optimization approach, complex investment alternatives, allowing for combinations of energy efficiency measures, can be analyzed. Uncertainties as well as time-dependent parameters, such as energy prices and policies, are modelled using a scenario-based approach, enabling the identification of robust investment solutions. The methodology is primarily an aid for decision-makers in industry, but it will also provide insight for policy-makers into how uncertainties regarding future price levels and policy instruments affect the decisions on investments in energy efficiency measures. (author)

An optimization methodology for identifying robust process integration investments under uncertainty

International Nuclear Information System (INIS)

Svensson, Elin; Berntsson, Thore; Stroemberg, Ann-Brith; Patriksson, Michael

2009-01-01

Uncertainties in future energy prices and policies strongly affect decisions on investments in process integration measures in industry. In this paper, we present a five-step methodology for the identification of robust investment alternatives incorporating explicitly such uncertainties in the optimization model. Methods for optimization under uncertainty (or, stochastic programming) are thus combined with a deep understanding of process integration and process technology in order to achieve a framework for decision-making concerning the investment planning of process integration measures under uncertainty. The proposed methodology enables the optimization of investments in energy efficiency with respect to their net present value or an environmental objective. In particular, as a result of the optimization approach, complex investment alternatives, allowing for combinations of energy efficiency measures, can be analyzed. Uncertainties as well as time-dependent parameters, such as energy prices and policies, are modelled using a scenario-based approach, enabling the identification of robust investment solutions. The methodology is primarily an aid for decision-makers in industry, but it will also provide insight for policy-makers into how uncertainties regarding future price levels and policy instruments affect the decisions on investments in energy efficiency measures. (author)

On the singular perturbations for fractional differential equation.

Science.gov (United States)

Atangana, Abdon

2014-01-01

The goal of this paper is to examine the possible extension of the singular perturbation differential equation to the concept of fractional order derivative. To achieve this, we presented a review of the concept of fractional calculus. We make use of the Laplace transform operator to derive exact solution of singular perturbation fractional linear differential equations. We make use of the methodology of three analytical methods to present exact and approximate solution of the singular perturbation fractional, nonlinear, nonhomogeneous differential equation. These methods are including the regular perturbation method, the new development of the variational iteration method, and the homotopy decomposition method.

Pulsed feedback defers cellular differentiation.

Directory of Open Access Journals (Sweden)

Joe H Levine

2012-01-01

Full Text Available Environmental signals induce diverse cellular differentiation programs. In certain systems, cells defer differentiation for extended time periods after the signal appears, proliferating through multiple rounds of cell division before committing to a new fate. How can cells set a deferral time much longer than the cell cycle? Here we study Bacillus subtilis cells that respond to sudden nutrient limitation with multiple rounds of growth and division before differentiating into spores. A well-characterized genetic circuit controls the concentration and phosphorylation of the master regulator Spo0A, which rises to a critical concentration to initiate sporulation. However, it remains unclear how this circuit enables cells to defer sporulation for multiple cell cycles. Using quantitative time-lapse fluorescence microscopy of Spo0A dynamics in individual cells, we observed pulses of Spo0A phosphorylation at a characteristic cell cycle phase. Pulse amplitudes grew systematically and cell-autonomously over multiple cell cycles leading up to sporulation. This pulse growth required a key positive feedback loop involving the sporulation kinases, without which the deferral of sporulation became ultrasensitive to kinase expression. Thus, deferral is controlled by a pulsed positive feedback loop in which kinase expression is activated by pulses of Spo0A phosphorylation. This pulsed positive feedback architecture provides a more robust mechanism for setting deferral times than constitutive kinase expression. Finally, using mathematical modeling, we show how pulsing and time delays together enable "polyphasic" positive feedback, in which different parts of a feedback loop are active at different times. Polyphasic feedback can enable more accurate tuning of long deferral times. Together, these results suggest that Bacillus subtilis uses a pulsed positive feedback loop to implement a "timer" that operates over timescales much longer than a cell cycle.

Advanced differential quadrature methods

CERN Document Server

Zong, Zhi

2009-01-01

Modern Tools to Perform Numerical DifferentiationThe original direct differential quadrature (DQ) method has been known to fail for problems with strong nonlinearity and material discontinuity as well as for problems involving singularity, irregularity, and multiple scales. But now researchers in applied mathematics, computational mechanics, and engineering have developed a range of innovative DQ-based methods to overcome these shortcomings. Advanced Differential Quadrature Methods explores new DQ methods and uses these methods to solve problems beyond the capabilities of the direct DQ method.After a basic introduction to the direct DQ method, the book presents a number of DQ methods, including complex DQ, triangular DQ, multi-scale DQ, variable order DQ, multi-domain DQ, and localized DQ. It also provides a mathematical compendium that summarizes Gauss elimination, the Runge-Kutta method, complex analysis, and more. The final chapter contains three codes written in the FORTRAN language, enabling readers to q...

Quantitative fluorescence spectroscopy in turbid media using fluorescence differential path length spectroscopy

NARCIS (Netherlands)

Amelink, Arjen; Kruijt, Bastiaan; Robinson, Dominic J.; Sterenborg, Henricus J. C. M.

2008-01-01

We have developed a new technique, fluorescence differential path length spectroscopy (FDPS), that enables the quantitative investigation of fluorophores in turbid media. FDPS measurements are made with the same probe geometry as differential path length spectroscopy (DPS) measurements. Phantom

Enabling cleanup technology transfer

International Nuclear Information System (INIS)

Ditmars, J. D.

2002-01-01

Technology transfer in the environmental restoration, or cleanup, area has been challenging. While there is little doubt that innovative technologies are needed to reduce the times, risks, and costs associated with the cleanup of federal sites, particularly those of the Departments of Energy (DOE) and Defense, the use of such technologies in actual cleanups has been relatively limited. There are, of course, many reasons why technologies do not reach the implementation phase or do not get transferred from developing entities to the user community. For example, many past cleanup contracts provided few incentives for performance that would compel a contractor to seek improvement via technology applications. While performance-based contracts are becoming more common, they alone will not drive increased technology applications. This paper focuses on some applications of cleanup methodologies and technologies that have been successful and are illustrative of a more general principle. The principle is at once obvious and not widely practiced. It is that, with few exceptions, innovative cleanup technologies are rarely implemented successfully alone but rather are implemented in the context of enabling processes and methodologies. And, since cleanup is conducted in a regulatory environment, the stage is better set for technology transfer when the context includes substantive interactions with the relevant stakeholders. Examples of this principle are drawn from Argonne National Laboratory's experiences in Adaptive Sampling and Analysis Programs (ASAPs), Precise Excavation, and the DOE Technology Connection (TechCon) Program. The lessons learned may be applicable to the continuing challenges posed by the cleanup and long-term stewardship of radioactive contaminants and unexploded ordnance (UXO) at federal sites

A total risk assessment methodology for security assessment

International Nuclear Information System (INIS)

Auilar, Richard; Pless, Daniel J.; Kaplan, Paul Garry; Silva, Consuelo Juanita; Rhea, Ronald Edward; Wyss, Gregory Dane; Conrad, Stephen Hamilton

2009-01-01

Sandia National Laboratories performed a two-year Laboratory Directed Research and Development project to develop a new collaborative risk assessment method to enable decision makers to fully consider the interrelationships between threat, vulnerability, and consequence. A five-step Total Risk Assessment Methodology was developed to enable interdisciplinary collaborative risk assessment by experts from these disciplines. The objective of this process is promote effective risk management by enabling analysts to identify scenarios that are simultaneously achievable by an adversary, desirable to the adversary, and of concern to the system owner or to society. The basic steps are risk identification, collaborative scenario refinement and evaluation, scenario cohort identification and risk ranking, threat chain mitigation analysis, and residual risk assessment. The method is highly iterative, especially with regard to scenario refinement and evaluation. The Total Risk Assessment Methodology includes objective consideration of relative attack likelihood instead of subjective expert judgment. The 'probability of attack' is not computed, but the relative likelihood for each scenario is assessed through identifying and analyzing scenario cohort groups, which are groups of scenarios with comparable qualities to the scenario being analyzed at both this and other targets. Scenarios for the target under consideration and other targets are placed into cohort groups under an established ranking process that reflects the following three factors: known targeting, achievable consequences, and the resources required for an adversary to have a high likelihood of success. The development of these target cohort groups implements, mathematically, the idea that adversaries are actively choosing among possible attack scenarios and avoiding scenarios that would be significantly suboptimal to their objectives. An adversary who can choose among only a few comparable targets and scenarios (a

Detection of differential viewing patterns to erotic and non-erotic stimuli using eye-tracking methodology.

Science.gov (United States)

Lykins, Amy D; Meana, Marta; Kambe, Gretchen

2006-10-01

As a first step in the investigation of the role of visual attention in the processing of erotic stimuli, eye-tracking methodology was employed to measure eye movements during erotic scene presentation. Because eye-tracking is a novel methodology in sexuality research, we attempted to determine whether the eye-tracker could detect differences (should they exist) in visual attention to erotic and non-erotic scenes. A total of 20 men and 20 women were presented with a series of erotic and non-erotic images and tracked their eye movements during image presentation. Comparisons between erotic and non-erotic image groups showed significant differences on two of three dependent measures of visual attention (number of fixations and total time) in both men and women. As hypothesized, there was a significant Stimulus x Scene Region interaction, indicating that participants visually attended to the body more in the erotic stimuli than in the non-erotic stimuli, as evidenced by a greater number of fixations and longer total time devoted to that region. These findings provide support for the application of eye-tracking methodology as a measure of visual attentional capture in sexuality research. Future applications of this methodology to expand our knowledge of the role of cognition in sexuality are suggested.

Entry and Competition in Differentiated Products Markets

NARCIS (Netherlands)

Schaumans, C.B.C.; Verboven, F.L.

2011-01-01

We propose a methodology for estimating the competition effects from entry when firms sell differentiated products. We first derive precise conditions under which Bres- nahan and Reiss'entry threshold ratios (ETRs) can be used to test for the presence and to measure the magnitude of competition

Differential expression of genes regulated in response to drought stress in diploid cotton (Gossypium arboreum) (abstract)

International Nuclear Information System (INIS)

Hussain, T.; Majeed, A.; Maqbool, A.; Hussain, S.S.; Ali, T.; Riazuddin, S.

2005-01-01

Negative effects on the Water status of plants is one of the most common and deleterious stresses experienced by wild and cultivated plants throughout the World. Our project is designed to identify, clone and characterize gene sequences regulated in response to Water stress (e.g., drought). We used the differential-display reverse transcriptase polymerase chain reaction (DD-RT- PCA) methodology to accomplish our Objectives. Structural and functional characterization of environmental stress-induced genes has contributed to a better understanding of how plants respond and adapt to different abiotic stresses. Differential display was used to compare overall difference in gene expression between draught stressed and unstressed (control) plants of diploid Cotton (Gossypium arboreum). DDRT-PCR product from stressed and unstressed samples resolved side by side on 6% PAGE to compare qualitative and quantitative difference in mRNA expression. A total of 81 primer combinations were tested. DDRT -PCR enabled us to identify differentially expressed transcripts between water stressed and non-stressed cotton seedlings. PAGE revealed a total of 347 DNA transcripts in stressed samples (New Transcripts) while 110 down regulated and 209 up regulated DNA transcripts were also recorded. Similarly. 22 DNA transcripts were identified based on the comparative study of PAGE and Agarose gel electrophoresis. These sequences showed various degree homology With draught tolerant genes in the gene bank. (author)

Methodology to estimating aquatic dispersion of effluents from accidental and routine releases

International Nuclear Information System (INIS)

Borges, Diogo da S.; Lava, Deise Diana; Guimarães, Antônio C.F.; Moreira, Maria L.

2017-01-01

This paper presents a methodology to analysis of dispersion of radioactive materials in an aquatic environment, specifically for estuaries, based on the Regulatory Guide 1.113. The objective is to present an adaptation of methodology for computational user, that it is possible by means of the use of numerical approximations techniques. The methodology to be present consist in a numerical approximation of the Navier-Stokes Equation applied in a finite medium with known transport mechanisms, such as Coriolis Effect, floor drag, diffusion, salinity, temperature difference and adhesion per water molecule. The basis of methodology is substantiated in a transport diffusive-convection equation, which has similarity with the Partial Differential Burgues' Equation for one dimension and with the Kardar-Parisi-Zhang Equation for multidimensional cases. (author)

Methodology to estimating aquatic dispersion of effluents from accidental and routine releases

Energy Technology Data Exchange (ETDEWEB)

Borges, Diogo da S.; Lava, Deise Diana; Guimarães, Antônio C.F.; Moreira, Maria L., E-mail: diogosb@outlook.com, E-mail: deise_dy@hotmail.com, E-mail: tony@ien.gov.br, E-mail: malu@ien.gov.br [Instituto de Engenharia Nuclear (IEN/CNEN-RJ), Rio de Janeiro, RJ (Brazil)

2017-07-01

This paper presents a methodology to analysis of dispersion of radioactive materials in an aquatic environment, specifically for estuaries, based on the Regulatory Guide 1.113. The objective is to present an adaptation of methodology for computational user, that it is possible by means of the use of numerical approximations techniques. The methodology to be present consist in a numerical approximation of the Navier-Stokes Equation applied in a finite medium with known transport mechanisms, such as Coriolis Effect, floor drag, diffusion, salinity, temperature difference and adhesion per water molecule. The basis of methodology is substantiated in a transport diffusive-convection equation, which has similarity with the Partial Differential Burgues' Equation for one dimension and with the Kardar-Parisi-Zhang Equation for multidimensional cases. (author)

A new methodology for determining dispersion coefficient using ordinary and partial differential transport equations.

Science.gov (United States)

Cho, Kyung Hwa; Lee, Seungwon; Ham, Young Sik; Hwang, Jin Hwan; Cha, Sung Min; Park, Yongeun; Kim, Joon Ha

2009-01-01

The present study proposes a methodology for determining the effective dispersion coefficient based on the field measurements performed in Gwangju (GJ) Creek in South Korea which is environmentally degraded by the artificial interferences such as weirs and culverts. Many previous works determining the dispersion coefficient were limited in application due to the complexity and artificial interferences in natural stream. Therefore, the sequential combination of N-Tank-In-Series (NTIS) model and Advection-Dispersion-Reaction (ADR) model was proposed for evaluating dispersion process in complex stream channel in this study. The series of water quality data were intensively monitored in the field to determine the effective dispersion coefficient of E. coli in rainy day. As a result, the suggested methodology reasonably estimates the dispersion coefficient for GJ Creek with 1.25 m(2)/s. Also, the sequential combined method provided Number of tank-Velocity-Dispersion coefficient (NVD) curves for convenient evaluation of dispersion coefficient of other rivers or streams. Comparing the previous studies, the present methodology is quite general and simple for determining the effective dispersion coefficients which are applicable for other rivers and streams.

From ordinary to partial differential equations

CERN Document Server

Esposito, Giampiero

2017-01-01

This book is addressed to mathematics and physics students who want to develop an interdisciplinary view of mathematics, from the age of Riemann, Poincaré and Darboux to basic tools of modern mathematics. It enables them to acquire the sensibility necessary for the formulation and solution of difficult problems, with an emphasis on concepts, rigour and creativity. It consists of eight self-contained parts: ordinary differential equations; linear elliptic equations; calculus of variations; linear and non-linear hyperbolic equations; parabolic equations; Fuchsian functions and non-linear equations; the functional equations of number theory; pseudo-differential operators and pseudo-differential equations. The author leads readers through the original papers and introduces new concepts, with a selection of topics and examples that are of high pedagogical value.

Application of the SAMINT methodology to the new cross section evaluations of 63Cu and 65Cu∗

Directory of Open Access Journals (Sweden)

Sobes Vladimir

2017-01-01

Full Text Available The SAMINT methodology allows coupling of differential and integral data evaluations in a continuous-energy framework. Prior to development of the SAMINT code, integral experimental data such as in the International Criticality Safety Benchmark Experiments Project remained a tool for validation of completed nuclear data evaluations. Now, SAMINT extracts information from integral benchmarks in the form of calculated sensitivity coefficients by Monte Carlo codes such as CE TSUNAMI-3D or MCNP6 and combines it with the results of experimental cross section measurements to produce an updated cross section evaluation utilizing information from both sets of data. The use of the generalized linear least squares methodology ensures that proper weight is given to both the differential and integral data. SAMINT is not intended to bias nuclear data toward specific integral experiments, but it should be used to supplement evaluation of differential experimental data. This work demonstrates the application of the SAMINT methodology to the new Oak Ridge National Laboratory (ORNL evaluations of the resonance parameters for two isotopes of copper: 63Cu and 65Cu.

Developing More Insights on Sustainable Consumption in China Based on Q Methodology

Directory of Open Access Journals (Sweden)

Ying Qu

2015-10-01

Full Text Available Being an important aspect of sustainable development, sustainable consumption has attracted great attention among Chinese politicians and academia, and Chinese governments have established policies that encourage sustainable consumption behaviors. However, unsustainable consumption behavior still remains predominant in China. This paper aims to classify consumers with similar traits, in terms of the characteristics of practicing sustainable consumption, into one group, so that their traits can be clearly understood, to enable governments to establish pointed policies for different groups of consumers. Q methodology, generally used to reveal the subjectivity of human beings involved in any situation, is applied in this paper to classify Chinese consumers based on Q sample design and data collection and analysis. Next, the traits of each group are analyzed in detail and comparison analyses are also conducted to compare the common and differentiating factors among the three groups. The results show that Chinese consumers can be classified into three groups: sustainable (Group 1, potential sustainable (Group 2 and unsustainable consumers (Group 3, according to their values and attitudes towards sustainable consumption. As such, Group 1 cares for the environment and has strong environmental values. They understand sustainable consumption and its functions. Group 2 needs more enlightenments and external stimuli to motivate them to consume sustainably. Group 3 needs to be informed about and educated on sustainable consumption to enable them to change their consumption behavior from unsustainable to sustainable. Suggestions and implications of encouraging each group of consumers to engage in sustainable consumption are also provided.

On the Singular Perturbations for Fractional Differential Equation

Directory of Open Access Journals (Sweden)

Abdon Atangana

2014-01-01

Full Text Available The goal of this paper is to examine the possible extension of the singular perturbation differential equation to the concept of fractional order derivative. To achieve this, we presented a review of the concept of fractional calculus. We make use of the Laplace transform operator to derive exact solution of singular perturbation fractional linear differential equations. We make use of the methodology of three analytical methods to present exact and approximate solution of the singular perturbation fractional, nonlinear, nonhomogeneous differential equation. These methods are including the regular perturbation method, the new development of the variational iteration method, and the homotopy decomposition method.

Consistency of differential and integral thermonuclear neutronics data

International Nuclear Information System (INIS)

Reupke, W.A.

1978-01-01

To increase the accuracy of the neutronics analysis of nuclear reactors, physicists and engineers have employed a variety of techniques, including the adjustment of multigroup differential data to improve consistency with integral data. Of the various adjustment strategies, a generalized least-squares procedure which adjusts the combined differential and integral data can significantly improve the accuracy of neutronics calculations compared to calculations employing only differential data. This investigation analyzes 14 MeV neutron-driven integral experiments, using a more extensively developed methodology and a newly developed computer code, to extend the domain of adjustment from the energy range of fission reactors to the energy range of fusion reactors

A grey DEMATEL-based approach for modeling enablers of green innovation in manufacturing organizations.

Science.gov (United States)

Gupta, Himanshu; Barua, Mukesh Kumar

2018-04-01

Incorporating green practices into the manufacturing process has gained momentum over the past few years and is a matter of great concern for both manufacturers as well as researchers. Regulatory pressures in developed countries have forced the organizations to adopt green practices; however, this issue still lacks attention in developing economies like India. There is an urgent need to identify enablers of green innovation for manufacturing organizations and also to identify prominent enablers among those. This study is an attempt to first identify enablers of green innovation and then establish a causal relationship among them to identify the enablers that can drive others. Grey DEMATEL (Decision Making Trial and Evaluation Laboratory) methodology is used for establishing the causal relationship among enablers. The novelty of this study lies in the fact that no study has been done in the past to identify the enablers of green innovation and then establishing the causal relationship among them. A total of 21 enablers of green innovation have been identified; research indicates developing green manufacturing capabilities, resources for green innovation, ease of getting loans from financial institutions, and environmental regulations as the most influential enablers of green innovation. Managerial and practical implications of the research are also presented to assist managers of the case company in adopting green innovation practices at their end.

Supply chain risk management enablers - A framework development through systematic review of the literature from 2000 to 2015

OpenAIRE

Kilubi, I.; Haasis, H.-D.

2015-01-01

The present paper delivers a robust and systematic literature review (SLR) on supply chain risk management (SCRM) with the purpose to a) review and analyse the literature concerning definitions and research methodologies applied, to b) develop a classificatory framework which clusters existing enablers on SCRM, and to c) examine the linkage between SCRM and performance. The findings reveal that not only is SCRM loosely defined, but that there are various fragmented supply chain risks enablers...

On simplified application of multidimensional Savitzky-Golay filters and differentiators

Science.gov (United States)

Shekhar, Chandra

2016-02-01

I propose a simplified approach for multidimensional Savitzky-Golay filtering, to enable its fast and easy implementation in scientific and engineering applications. The proposed method, which is derived from a generalized framework laid out by Thornley (D. J. Thornley, "Novel anisotropic multidimensional convolution filters for derivative estimation and reconstruction" in Proceedings of International Conference on Signal Processing and Communications, November 2007), first transforms any given multidimensional problem into a unique one, by transforming coordinates of the sampled data nodes to unity-spaced, uniform data nodes, and then performs filtering and calculates partial derivatives on the unity-spaced nodes. It is followed by transporting the calculated derivatives back onto the original data nodes by using the chain rule of differentiation. The burden to performing the most cumbersome task, which is to carry out the filtering and to obtain derivatives on the unity-spaced nodes, is almost eliminated by providing convolution coefficients for a number of convolution kernel sizes and polynomial orders, up to four spatial dimensions. With the availability of the convolution coefficients, the task of filtering at a data node reduces merely to multiplication of two known matrices. Simplified strategies to adequately address near-boundary data nodes and to calculate partial derivatives there are also proposed. Finally, the proposed methodologies are applied to a three-dimensional experimentally obtained data set, which shows that multidimensional Savitzky-Golay filters and differentiators perform well in both the internal and the near-boundary regions of the domain.

Introduction to Differential Calculus Systematic Studies with Engineering Applications for Beginners

CERN Document Server

Rohde, Ulrich L; Poddar, Ajay K; Ghosh, A K

2011-01-01

Enables readers to apply the fundamentals of differential calculus to solve real-life problems in engineering and the physical sciences Introduction to Differential Calculus fully engages readers by presenting the fundamental theories and methods of differential calculus and then showcasing how the discussed concepts can be applied to real-world problems in engineering and the physical sciences. With its easy-to-follow style and accessible explanations, the book sets a solid foundation before advancing to specific calculus methods, demonstrating the connections between differential calculus

Hard Times for HRD, Lean Times for Learning?: Workplace Participatory Practices as Enablers of Learning

Science.gov (United States)

Warhurst, Russell

2013-01-01

Purpose: This article aims to show how in times of austerity when formal HRD activity is curtailed and yet the need for learning is greatest, non-formal learning methods such as workplace involvement and participation initiated by line managers can compensate by enabling the required learning and change. Design/methodology/approach: A qualitative…

Human Schedule Performance, Protocol Analysis, and the "Silent Dog" Methodology

Science.gov (United States)

Cabello, Francisco; Luciano, Carmen; Gomez, Inmaculada; Barnes-Holmes, Dermot

2004-01-01

The purpose of the current experiment was to investigate the role of private verbal behavior on the operant performances of human adults, using a protocol analysis procedure with additional methodological controls (the "silent dog" method). Twelve subjects were exposed to fixed ratio 8 and differential reinforcement of low rate 3-s schedules. For…

Technologies, Methodologies and Challenges in Network Intrusion Detection and Prevention Systems

Directory of Open Access Journals (Sweden)

Nicoleta STANCIU

2013-01-01

Full Text Available This paper presents an overview of the technologies and the methodologies used in Network Intrusion Detection and Prevention Systems (NIDPS. Intrusion Detection and Prevention System (IDPS technologies are differentiated by types of events that IDPSs can recognize, by types of devices that IDPSs monitor and by activity. NIDPSs monitor and analyze the streams of network packets in order to detect security incidents. The main methodology used by NIDPSs is protocol analysis. Protocol analysis requires good knowledge of the theory of the main protocols, their definition, how each protocol works.

Audit Methodology for IT Governance

Directory of Open Access Journals (Sweden)

Mirela GHEORGHE

2010-01-01

Full Text Available The continuous development of the new IT technologies was followed up by a rapid integration of them at the organization level. The management of the organizations face a new challenge: structural redefinition of the IT component in order to create plus value and to minimize IT risks through an efficient management of all IT resources of the organization. These changes have had a great impact on the governance of the IT component. The paper proposes an audit methodology of the IT Governance at the organization level. From this point of view the developed audit strategy is a strategy based on risks to enable IT auditor to study from the best angle efficiency and effectiveness of the IT Governance structure. The evaluation of the risks associated with IT Governance is a key process in planning the audit mission which will allow the identification of the segments with increased risks. With now ambition for completeness, the proposed methodology provides the auditor a useful tool in the accomplishment of his mission.

Large deformation and mechanics of flexible isotropic membrane ballooning in three dimensions by differential quadrature method

International Nuclear Information System (INIS)

Mozaffari, M.; Atai, A. A.; Mostafa, N.

2009-01-01

This paper presents a computationally efficient and accurate new methodology in the differential quadrature analysis of structural mechanics for flexible membranes ballooning in three dimensions under a negative air pressure differential. The differential quadrature method is employed to discretize the resulting equations in the axial direction as well as for the solution procedure. The weighting coefficients employed are not exclusive, and any accurate and efficient method such as the generalized differential quadrature method may be used to produce the methods weighting coefficients. A second-order paraboloid of revolution is assumed to describe the ballooning shape. This study asserts the accuracy, convergency, and efficiency of the methodology by solving some typical stability, straining analysis membrane problems, and comparing the results with those of the exact solutions and/or those of physical tests

Large deformation and mechanics of flexible isotropic membrane ballooning in three dimensions by differential quadrature method

Energy Technology Data Exchange (ETDEWEB)

Mozaffari, M.; Atai, A. A.; Mostafa, N. [Islamic Azad University, Karaj (Iran, Islamic Republic of)

2009-11-15

This paper presents a computationally efficient and accurate new methodology in the differential quadrature analysis of structural mechanics for flexible membranes ballooning in three dimensions under a negative air pressure differential. The differential quadrature method is employed to discretize the resulting equations in the axial direction as well as for the solution procedure. The weighting coefficients employed are not exclusive, and any accurate and efficient method such as the generalized differential quadrature method may be used to produce the methods weighting coefficients. A second-order paraboloid of revolution is assumed to describe the ballooning shape. This study asserts the accuracy, convergency, and efficiency of the methodology by solving some typical stability, straining analysis membrane problems, and comparing the results with those of the exact solutions and/or those of physical tests

The Methodological Imperatives of Feminist Ethnography

Directory of Open Access Journals (Sweden)

Richelle D. Schrock

2013-12-01

Full Text Available Feminist ethnography does not have a single, coherent definition and is caught between struggles over the definition and goals of feminism and the multiple practices known collectively as ethnography. Towards the end of the 1980s, debates emerged that problematized feminist ethnography as a productive methodology and these debates still haunt feminist ethnographers today. In this article, I provide a concise historiography of feminist ethnography that summarizes both its promises and its vulnerabilities. I address the three major challenges I argue feminist ethnographers currently face, which include responding productively to feminist critiques of representing "others," accounting for feminisms' commitment to social change while grappling with poststructuralist critiques of knowledge production, and confronting the historical and ongoing lack of recognition for significant contributions by feminist ethnographers. Despite these challenges, I argue that feminist ethnography is a productive methodology and I conclude by delineating its methodological imperatives. These imperatives include producing knowledge about women's lives in specific cultural contexts, recognizing the potential detriments and benefits of representation, exploring women's experiences of oppression along with the agency they exercise in their own lives, and feeling an ethical responsibility towards the communities in which the researchers work. I argue that this set of imperatives enables feminist ethnographers to successfully navigate the challenges they face.

Soil Radiological Characterisation Methodology

International Nuclear Information System (INIS)

Attiogbe, Julien; Aubonnet, Emilie; De Maquille, Laurence; De Moura, Patrick; Desnoyers, Yvon; Dubot, Didier; Feret, Bruno; Fichet, Pascal; Granier, Guy; Iooss, Bertrand; Nokhamzon, Jean-Guy; Ollivier Dehaye, Catherine; Pillette-Cousin, Lucien; Savary, Alain

2014-12-01

This report presents the general methodology and best practice approaches which combine proven existing techniques for sampling and characterisation to assess the contamination of soils prior to remediation. It is based on feedback of projects conducted by main French nuclear stakeholders involved in the field of remediation and dismantling (EDF, CEA, AREVA and IRSN). The application of this methodology will enable the project managers to obtain the elements necessary for the drawing up of files associated with remediation operations, as required by the regulatory authorities. It is applicable to each of the steps necessary for the piloting of remediation work-sites, depending on the objectives targeted (release into the public domain, re-use, etc.). The main part describes the applied statistical methodology with the exploratory analysis and variogram data, identification of singular points and their location. The results obtained permit assessment of a mapping to identify the contaminated surface and subsurface areas. It stakes the way for radiological site characterisation since the initial investigations from historical and functional analysis to check that the remediation objectives have been met. It follows an example application from the feedback of the remediation of a contaminated site on the Fontenay aux Roses facility. It is supplemented by a glossary of main terms used in the field from different publications or international standards. This technical report is a support of the ISO Standard ISO ISO/TC 85/SC 5 N 18557 'Sampling and characterisation principles for soils, buildings and infrastructures contaminated by radionuclides for remediation purposes'. (authors) [fr

Highly efficient differentiation of embryonic stem cells into adipocytes by ascorbic acid

OpenAIRE

Ixchelt Cuaranta-Monroy; Zoltan Simandi; Zsuzsanna Kolostyak; Quang-Minh Doan-Xuan; Szilard Poliska; Attila Horvath; Gergely Nagy; Zsolt Bacso; Laszlo Nagy

2014-01-01

Adipocyte differentiation and function have become the major research targets due to the increasing interest in obesity and related metabolic conditions. Although, late stages of adipogenesis have been extensively studied, the early phases remain poorly understood. Here we present that supplementing ascorbic acid (AsA) to the adipogenic differentiation cocktail enables the robust and efficient differentiation of mouse embryonic stem cells (mESCs) to mature adipocytes. Such ESC-derived adipocy...

Nonlinear Observer Design of the Generalized Rössler Hyperchaotic Systems via DIL Methodology

Directory of Open Access Journals (Sweden)

Yeong-Jeu Sun

2012-01-01

Full Text Available The generalized Rössler hyperchaotic systems are presented, and the state observation problem of such systems is investigated. Based on the differential inequality with Lyapunov methodology (DIL methodology, a nonlinear observer design for the generalized Rössler hyperchaotic systems is developed to guarantee the global exponential stability of the resulting error system. Meanwhile, the guaranteed exponential decay rate can be accurately estimated. Finally, numerical simulations are provided to illustrate the feasibility and effectiveness of proposed approach.

A probabilistic seismic risk assessment procedure for nuclear power plants: (I) Methodology

Science.gov (United States)

Huang, Y.-N.; Whittaker, A.S.; Luco, N.

2011-01-01

A new procedure for probabilistic seismic risk assessment of nuclear power plants (NPPs) is proposed. This procedure modifies the current procedures using tools developed recently for performance-based earthquake engineering of buildings. The proposed procedure uses (a) response-based fragility curves to represent the capacity of structural and nonstructural components of NPPs, (b) nonlinear response-history analysis to characterize the demands on those components, and (c) Monte Carlo simulations to determine the damage state of the components. The use of response-rather than ground-motion-based fragility curves enables the curves to be independent of seismic hazard and closely related to component capacity. The use of Monte Carlo procedure enables the correlation in the responses of components to be directly included in the risk assessment. An example of the methodology is presented in a companion paper to demonstrate its use and provide the technical basis for aspects of the methodology. ?? 2011 Published by Elsevier B.V.

Energy efficiency with QoS control in dynamic optical networks with SDN enabled integrated control plane

DEFF Research Database (Denmark)

Wang, Jiayuan; Chen, Xin; Phillips, Chris

2014-01-01

The paper presents energy efficient routing algorithms based on a novel integrated control plane platform. The centralized control plane structure enables the use of flexible heuristic algorithms for route selection in optical networks. Differentiated routing for various traffic types is used in ...

A computational methodology for formulating gasoline surrogate fuels with accurate physical and chemical kinetic properties

KAUST Repository

Ahmed, Ahfaz; Goteng, Gokop; Shankar, Vijai; Al-Qurashi, Khalid; Roberts, William L.; Sarathy, Mani

2015-01-01

simpler molecular composition that represent real fuel behavior in one or more aspects are needed to enable repeatable experimental and computational combustion investigations. This study presents a novel computational methodology for formulating

Methodology for Validation of Housing Standards - the Kitchen as an Example

DEFF Research Database (Denmark)

Helle, Tina; Iwarsson, Susanne; Brandt, Åse

We will present a novel methodology to validate housing standards. Person-environment fit among older persons with different levels of mobility device dependence was investigated during performance of everyday activities in realistic environments. Observational and self-report data were compared...... difficulties in rating accessibility problems. The actual use of mobility devices form part of the activity performance. Some standards are not defined properly to enable activity performance in realistic environments. After attending this activity, participants will be: • Aware of some of the methodological...... challenges concerning sampling and the measurement of accessibility problems among older people with different levels of dependence in mobility device use • Aware of the implications of using observation versus self-report data in research on person-environment fit • Consider the methodological implications...

The KNOMAD Methodology for Integration of Multi-Disciplinary Engineering Knowledge within Aerospace Production

NARCIS (Netherlands)

Curran, R.; Verhagen, W.J.C.; Van Tooren, M.J.L.

2010-01-01

The paper is associated with the integration of multi-disciplinary knowledge within a Knowledge Based Engineering (KBE)-enabled design framework. To support this integration effort, the KNOMAD methodology has been devised. KNOMAD stands for Knowledge Optimized Manufacture And Design and is a

Application of survival analysis methodology to the quantitative analysis of LC-MS proteomics data

KAUST Repository

Tekwe, C. D.; Carroll, R. J.; Dabney, A. R.

2012-01-01

positive, skewed and often left-censored, we propose using survival methodology to carry out differential expression analysis of proteins. Various standard statistical techniques including non-parametric tests such as the Kolmogorov-Smirnov and Wilcoxon

Determining Regulatory Networks Governing the Differentiation of Embryonic Stem Cells to Pancreatic Lineage

Science.gov (United States)

Banerjee, Ipsita

2009-03-01

Knowledge of pathways governing cellular differentiation to specific phenotype will enable generation of desired cell fates by careful alteration of the governing network by adequate manipulation of the cellular environment. With this aim, we have developed a novel method to reconstruct the underlying regulatory architecture of a differentiating cell population from discrete temporal gene expression data. We utilize an inherent feature of biological networks, that of sparsity, in formulating the network reconstruction problem as a bi-level mixed-integer programming problem. The formulation optimizes the network topology at the upper level and the network connectivity strength at the lower level. The method is first validated by in-silico data, before applying it to the complex system of embryonic stem (ES) cell differentiation. This formulation enables efficient identification of the underlying network topology which could accurately predict steps necessary for directing differentiation to subsequent stages. Concurrent experimental verification demonstrated excellent agreement with model prediction.

Differentially Private Distributed Sensing

Energy Technology Data Exchange (ETDEWEB)

Fink, Glenn A.

2016-12-11

The growth of the Internet of Things (IoT) creates the possibility of decentralized systems of sensing and actuation, potentially on a global scale. IoT devices connected to cloud networks can offer Sensing and Actuation as a Service (SAaaS) enabling networks of sensors to grow to a global scale. But extremely large sensor networks can violate privacy, especially in the case where IoT devices are mobile and connected directly to the behaviors of people. The thesis of this paper is that by adapting differential privacy (adding statistically appropriate noise to query results) to groups of geographically distributed sensors privacy could be maintained without ever sending all values up to a central curator and without compromising the overall accuracy of the data collected. This paper outlines such a scheme and performs an analysis of differential privacy techniques adapted to edge computing in a simulated sensor network where ground truth is known. The positive and negative outcomes of employing differential privacy in distributed networks of devices are discussed and a brief research agenda is presented.

Methodology for Designing Operational Banking Risks Monitoring System

Science.gov (United States)

Kostjunina, T. N.

2018-05-01

The research looks at principles of designing an information system for monitoring operational banking risks. A proposed design methodology enables one to automate processes of collecting data on information security incidents in the banking network, serving as the basis for an integrated approach to the creation of an operational risk management system. The system can operate remotely ensuring tracking and forecasting of various operational events in the bank network. A structure of a content management system is described.

Directory Enabled Policy Based Networking; TOPICAL

International Nuclear Information System (INIS)

KELIIAA, CURTIS M.

2001-01-01

This report presents a discussion of directory-enabled policy-based networking with an emphasis on its role as the foundation for securely scalable enterprise networks. A directory service provides the object-oriented logical environment for interactive cyber-policy implementation. Cyber-policy implementation includes security, network management, operational process and quality of service policies. The leading network-technology vendors have invested in these technologies for secure universal connectivity that transverses Internet, extranet and intranet boundaries. Industry standards are established that provide the fundamental guidelines for directory deployment scalable to global networks. The integration of policy-based networking with directory-service technologies provides for intelligent management of the enterprise network environment as an end-to-end system of related clients, services and resources. This architecture allows logical policies to protect data, manage security and provision critical network services permitting a proactive defense-in-depth cyber-security posture. Enterprise networking imposes the consideration of supporting multiple computing platforms, sites and business-operation models. An industry-standards based approach combined with principled systems engineering in the deployment of these technologies allows these issues to be successfully addressed. This discussion is focused on a directory-based policy architecture for the heterogeneous enterprise network-computing environment and does not propose specific vendor solutions. This document is written to present practical design methodology and provide an understanding of the risks, complexities and most important, the benefits of directory-enabled policy-based networking

Mapping industrial systems - a supply network perspective on enabling technologies, processes and actors

OpenAIRE

Srai, Jagjit Singh

2016-01-01

This is the author accepted manuscript. The final version is available from InderScience Publishers via http://dx.doi.org/10.1504/IJMTM.2017.10002927 This paper develops a multi-layered multi-stage mapping approach to explore the characteristics of emerging industry supply networks (EI SNs), and how enabling production technologies and supply chain processes are supported by institutional, industrial and supply network actors. The mapping methodology involves the systematic capture of mate...

An auxiliary differential equation FDTD method for anisotropic magnetized plasmas

International Nuclear Information System (INIS)

Liu Shaobin; Mo Jinjun; Yuan Naichang

2004-01-01

An auxiliary differential equation finite-difference time-domain (ADE-FDTD) methodology for anisotropic magnetized plasmas is derived. The method is based on a difference approximation of the auxiliary differential equation. A comparison with the JEC method is included. The CPU time saving by several times and accuracy of the method are confirmed by computing the reflection and transmission through a magnetized plasma layer with the direction of propagation parallel to the direction of the biasing field

Methodology for national risk analysis and prioritization of toxic industrial chemicals.

Science.gov (United States)

Taxell, Piia; Engström, Kerstin; Tuovila, Juha; Söderström, Martin; Kiljunen, Harri; Vanninen, Paula; Santonen, Tiina

2013-01-01

The identification of chemicals that pose the greatest threat to human health from incidental releases is a cornerstone in public health preparedness for chemical threats. The present study developed and applied a methodology for the risk analysis and prioritization of industrial chemicals to identify the most significant chemicals that pose a threat to public health in Finland. The prioritization criteria included acute and chronic health hazards, physicochemical and environmental hazards, national production and use quantities, the physicochemical properties of the substances, and the history of substance-related incidents. The presented methodology enabled a systematic review and prioritization of industrial chemicals for the purpose of national public health preparedness for chemical incidents.

Delayed puberty and hypogonadotropic hypogonadism. Differential diagnosis and treatment

NARCIS (Netherlands)

Snoep, Marinus Cornelis

1978-01-01

This thesis describes a method enabling a prospecrive differential diagnosis to be made berween delayed puberty (DP) and hypogonadotropic hypogonadism (HH). The influence of androgen administration on the gonadal feedback sysrem of patients with delayed puberty was also studied. ... Zie: Summary

Forming professional mobility of a future teacher: methodological approaches

Directory of Open Access Journals (Sweden)

Prima Raisa Nikolaevna

2011-10-01

Full Text Available Analyzes the theoretical and methodological principles of occupational mobility of future teachers. Focuses on the motivation of the choice of systems methodology, the "system integrity" as the dominant conceptual study. It is noted that the educational process of higher education provides an opportunity: the implementation of integration and differentiation of learning content, technology, formation of professional mobility, the stimulating activity of quasi professional future teachers, mainstream interdisciplinary connections. It is established that the formation of a mobile professional organization provides some of the "field capacity", education and upbringing environment, life. It focuses on the future of professional diversity of choice of the status of growth, professional development, rapid orientation (adapted, actively striving to change the unfavorable situation.

Enabling optimization in LCA: from “ad hoc” to “structural” LCA approach—based on a biodiesel well-to-wheel case study

DEFF Research Database (Denmark)

Herrmann, Ivan Tengbjerg; Lundberg-Jensen, Martin; Jørgensen, Andreas

2014-01-01

for searching or screening product systems for environmental optimization potentials. In the presented case, the design has been a rather simple full factorial design. More complicated problems or designs, such as fractional designs, nested designs, split plot designs, and/or unbalanced data, in the context...... 2005). Through a biodiesel well-to-wheel study, we demonstrate a generic approach of applying explanatory variables and corresponding impact categories within the LCA methodology. Explanatory variables are product system variables that can influence the environmental impacts from the system....... Furthermore, using the structural approach enables two different possibilities for optimization: (1) single-objective optimization (SO) based on response surface methodology (Montgomery 2005) and (2) multiobjective optimization (MO) by the hypervolume estimation taboo search (HETS) method. HETS enables MO...

Methodology for evaluating pattern transfer completeness in inkjet printing with irregular edges

Science.gov (United States)

Huang, Bo-Cin; Chan, Hui-Ju; Hong, Jian-Wei; Lo, Cheng-Yao

2016-06-01

A methodology for quantifying and qualifying pattern transfer completeness in inkjet printing through examining both pattern dimensions and pattern contour deviations from reference design is proposed, which enables scientifically identifying and evaluating inkjet-printed lines, corners, circles, ellipses, and spirals with irregular edges of bulging, necking, and unpredictable distortions resulting from different process conditions. This methodology not only avoids differences in individual perceptions of ambiguous pattern distortions but also indicates the systematic effects of mechanical stresses applied in different directions to a polymer substrate, and is effective for both optical and electrical microscopy in direct and indirect lithography or lithography-free patterning.

Methodology for evaluating pattern transfer completeness in inkjet printing with irregular edges

International Nuclear Information System (INIS)

Huang, Bo-Cin; Chan, Hui-Ju; Lo, Cheng-Yao; Hong, Jian-Wei

2016-01-01

A methodology for quantifying and qualifying pattern transfer completeness in inkjet printing through examining both pattern dimensions and pattern contour deviations from reference design is proposed, which enables scientifically identifying and evaluating inkjet-printed lines, corners, circles, ellipses, and spirals with irregular edges of bulging, necking, and unpredictable distortions resulting from different process conditions. This methodology not only avoids differences in individual perceptions of ambiguous pattern distortions but also indicates the systematic effects of mechanical stresses applied in different directions to a polymer substrate, and is effective for both optical and electrical microscopy in direct and indirect lithography or lithography-free patterning. (paper)

Design of a rotary reactor for chemical-looping combustion. Part 1: Fundamentals and design methodology

KAUST Repository

Zhao, Zhenlong; Iloeje, Chukwunwike O.; Chen, Tianjiao; Ghoniem, Ahmed F.

2014-01-01

of the OC characteristics, the design parameters, and the operating conditions are studied. The design procedures are presented on the basis of the relative importance of each parameter, enabling a systematic methodology of selecting the design parameters

Solving differential–algebraic equation systems by means of index reduction methodology

DEFF Research Database (Denmark)

Sørensen, Kim; Houbak, Niels; Condra, Thomas

2006-01-01

of a number of differential equations and algebraic equations — a so called DAE system. Two of the DAE systems are of index 1 and they can be solved by means of standard DAE-solvers. For the actual application, the equation systems are integrated by means of MATLAB’s solver: ode23t, that solves moderately...... stiff ODEs and index 1 DAEs by means of the trapezoidal rule. The last sub-model that models the boilers steam drum consist of two differential and three algebraic equations. The index of this model is greater than 1, which means that ode23t cannot integrate this equation system. In this paper......, it is shown how the equation system, by means of an index reduction methodology, can be reduced to a system of ordinary differential equations — ODEs....

Optimal control of motorsport differentials

Science.gov (United States)

Tremlett, A. J.; Massaro, M.; Purdy, D. J.; Velenis, E.; Assadian, F.; Moore, A. P.; Halley, M.

2015-12-01

Modern motorsport limited slip differentials (LSD) have evolved to become highly adjustable, allowing the torque bias that they generate to be tuned in the corner entry, apex and corner exit phases of typical on-track manoeuvres. The task of finding the optimal torque bias profile under such varied vehicle conditions is complex. This paper presents a nonlinear optimal control method which is used to find the minimum time optimal torque bias profile through a lane change manoeuvre. The results are compared to traditional open and fully locked differential strategies, in addition to considering related vehicle stability and agility metrics. An investigation into how the optimal torque bias profile changes with reduced track-tyre friction is also included in the analysis. The optimal LSD profile was shown to give a performance gain over its locked differential counterpart in key areas of the manoeuvre where a quick direction change is required. The methodology proposed can be used to find both optimal passive LSD characteristics and as the basis of a semi-active LSD control algorithm.

A methodology for radiological accidents analysis in industrial gamma radiography

International Nuclear Information System (INIS)

Silva, F.C.A. da.

1990-01-01

A critical review of 34 published severe radiological accidents in industrial gamma radiography, that happened in 15 countries, from 1960 to 1988, was performed. The most frequent causes, consequences and dose estimation methods were analysed, aiming to stablish better procedures of radiation safety and accidents analysis. The objective of this work is to elaborate a radiological accidents analysis methodology in industrial gamma radiography. The suggested methodology will enable professionals to determine the true causes of the event and to estimate the dose with a good certainty. The technical analytical tree, recommended by International Atomic Energy Agency to perform radiation protection and nuclear safety programs, was adopted in the elaboration of the suggested methodology. The viability of the use of the Electron Gamma Shower 4 Computer Code System to calculate the absorbed dose in radiological accidents in industrial gamma radiography, mainly at sup(192)Ir radioactive source handling situations was also studied. (author)

A validated methodology for genetic identification of tuna species (genus Thunnus.

Directory of Open Access Journals (Sweden)

Jordi Viñas

2009-10-01

Full Text Available Tuna species of the genus Thunnus, such as the bluefin tunas, are some of the most important and yet most endangered trade fish in the world. Identification of these species in traded forms, however, may be difficult depending on the presentation of the products, which may hamper conservation efforts on trade control. In this paper, we validated a genetic methodology that can fully distinguish between the eight Thunnus species from any kind of processed tissue.After testing several genetic markers, a complete discrimination of the eight tuna species was achieved using Forensically Informative Nucleotide Sequencing based primarily on the sequence variability of the hypervariable genetic marker mitochondrial DNA control region (mtDNA CR, followed, in some specific cases, by a second validation by a nuclear marker rDNA first internal transcribed spacer (ITS1. This methodology was able to distinguish all tuna species, including those belonging to the subgenus Neothunnus that are very closely related, and in consequence can not be differentiated with other genetic markers of lower variability. This methodology also took into consideration the presence of introgression that has been reported in past studies between T. thynnus, T. orientalis and T. alalunga. Finally, we applied the methodology to cross-check the species identity of 26 processed tuna samples.Using the combination of two genetic markers, one mitochondrial and another nuclear, allows a full discrimination between all eight tuna species. Unexpectedly, the genetic marker traditionally used for DNA barcoding, cytochrome oxidase 1, could not differentiate all species, thus its use as a genetic marker for tuna species identification is questioned.

Differential forms for scientists and engineers

Science.gov (United States)

Blair Perot, J.; Zusi, Christopher J.

2014-01-01

This paper is a review of a number of mathematical concepts from differential geometry and exterior calculus that are finding increasing application in the numerical solution of partial differential equations. The objective of the paper is to introduce the scientist/ engineer to some of these ideas via a number of concrete examples in 2, 3, and 4 dimensions. The goal is not to explain these ideas with mathematical precision but to present concrete examples and enable a physical intuition of these concepts for those who are not mathematicians. The objective of this paper is to provide enough context so that scientist/engineers can interpret, implement, and understand other works which use these elegant mathematical concepts.

A Global Sensitivity Analysis Methodology for Multi-physics Applications

Energy Technology Data Exchange (ETDEWEB)

Tong, C H; Graziani, F R

2007-02-02

Experiments are conducted to draw inferences about an entire ensemble based on a selected number of observations. This applies to both physical experiments as well as computer experiments, the latter of which are performed by running the simulation models at different input configurations and analyzing the output responses. Computer experiments are instrumental in enabling model analyses such as uncertainty quantification and sensitivity analysis. This report focuses on a global sensitivity analysis methodology that relies on a divide-and-conquer strategy and uses intelligent computer experiments. The objective is to assess qualitatively and/or quantitatively how the variabilities of simulation output responses can be accounted for by input variabilities. We address global sensitivity analysis in three aspects: methodology, sampling/analysis strategies, and an implementation framework. The methodology consists of three major steps: (1) construct credible input ranges; (2) perform a parameter screening study; and (3) perform a quantitative sensitivity analysis on a reduced set of parameters. Once identified, research effort should be directed to the most sensitive parameters to reduce their uncertainty bounds. This process is repeated with tightened uncertainty bounds for the sensitive parameters until the output uncertainties become acceptable. To accommodate the needs of multi-physics application, this methodology should be recursively applied to individual physics modules. The methodology is also distinguished by an efficient technique for computing parameter interactions. Details for each step will be given using simple examples. Numerical results on large scale multi-physics applications will be available in another report. Computational techniques targeted for this methodology have been implemented in a software package called PSUADE.

Methodology for Designing Fault-Protection Software

Science.gov (United States)

Barltrop, Kevin; Levison, Jeffrey; Kan, Edwin

2006-01-01

A document describes a methodology for designing fault-protection (FP) software for autonomous spacecraft. The methodology embodies and extends established engineering practices in the technical discipline of Fault Detection, Diagnosis, Mitigation, and Recovery; and has been successfully implemented in the Deep Impact Spacecraft, a NASA Discovery mission. Based on established concepts of Fault Monitors and Responses, this FP methodology extends the notion of Opinion, Symptom, Alarm (aka Fault), and Response with numerous new notions, sub-notions, software constructs, and logic and timing gates. For example, Monitor generates a RawOpinion, which graduates into Opinion, categorized into no-opinion, acceptable, or unacceptable opinion. RaiseSymptom, ForceSymptom, and ClearSymptom govern the establishment and then mapping to an Alarm (aka Fault). Local Response is distinguished from FP System Response. A 1-to-n and n-to- 1 mapping is established among Monitors, Symptoms, and Responses. Responses are categorized by device versus by function. Responses operate in tiers, where the early tiers attempt to resolve the Fault in a localized step-by-step fashion, relegating more system-level response to later tier(s). Recovery actions are gated by epoch recovery timing, enabling strategy, urgency, MaxRetry gate, hardware availability, hazardous versus ordinary fault, and many other priority gates. This methodology is systematic, logical, and uses multiple linked tables, parameter files, and recovery command sequences. The credibility of the FP design is proven via a fault-tree analysis "top-down" approach, and a functional fault-mode-effects-and-analysis via "bottoms-up" approach. Via this process, the mitigation and recovery strategy(s) per Fault Containment Region scope (width versus depth) the FP architecture.

Do systematic reviews on pediatric topics need special methodological considerations?

OpenAIRE

Farid-Kapadia, Mufiza; Askie, Lisa; Hartling, Lisa; Contopoulos-Ioannidis, Despina; Bhutta, Zulfiqar A.; Soll, Roger; Moher, David; Offringa, Martin

2017-01-01

Background Systematic reviews are key tools to enable decision making by healthcare providers and policymakers. Despite the availability of the evidence based Preferred Reporting Items for Systematic reviews and Meta-Analysis (PRISMA-2009 and PRISMA-P 2015) statements that were developed to improve the transparency and quality of reporting of systematic reviews, uncertainty on how to deal with pediatric-specific methodological challenges of systematic reviews impairs decision-making in child ...

Current status and future prospects for enabling chemistry technology in the drug discovery process.

Science.gov (United States)

Djuric, Stevan W; Hutchins, Charles W; Talaty, Nari N

2016-01-01

This review covers recent advances in the implementation of enabling chemistry technologies into the drug discovery process. Areas covered include parallel synthesis chemistry, high-throughput experimentation, automated synthesis and purification methods, flow chemistry methodology including photochemistry, electrochemistry, and the handling of "dangerous" reagents. Also featured are advances in the "computer-assisted drug design" area and the expanding application of novel mass spectrometry-based techniques to a wide range of drug discovery activities.

Multivariate analysis of microarray data: differential expression and differential connection.

Science.gov (United States)

Kiiveri, Harri T

2011-02-01

Typical analysis of microarray data ignores the correlation between gene expression values. In this paper we present a model for microarray data which specifically allows for correlation between genes. As a result we combine gene network ideas with linear models and differential expression. We use sparse inverse covariance matrices and their associated graphical representation to capture the notion of gene networks. An important issue in using these models is the identification of the pattern of zeroes in the inverse covariance matrix. The limitations of existing methods for doing this are discussed and we provide a workable solution for determining the zero pattern. We then consider a method for estimating the parameters in the inverse covariance matrix which is suitable for very high dimensional matrices. We also show how to construct multivariate tests of hypotheses. These overall multivariate tests can be broken down into two components, the first one being similar to tests for differential expression and the second involving the connections between genes. The methods in this paper enable the extraction of a wealth of information concerning the relationships between genes which can be conveniently represented in graphical form. Differentially expressed genes can be placed in the context of the gene network and places in the gene network where unusual or interesting patterns have emerged can be identified, leading to the formulation of hypotheses for future experimentation.

Fundamentals of clinical methodology: 2. Etiology.

Science.gov (United States)

Sadegh-Zadeh, K

1998-03-01

The concept of etiology is analyzed and the possibilities and limitations of deterministic, probabilistic, and fuzzy etiology are explored. Different kinds of formal structures for the relation of causation are introduced which enable us to explicate the notion of cause on qualitative, comparative, and quantitative levels. The conceptual framework developed is an approach to a theory of causality that may be useful in etiologic research, in building nosological systems, and in differential diagnosis, therapeutic decision-making, and controlled clinical trials. The bearings of the theory are exemplified by examining the current Chlamydia pneumoniae hypothesis on the incidence of myocardial infarction.

A diagnostic methodology for refrigerating systems; Methodologie de diagnostic des installations frigorifiques

Energy Technology Data Exchange (ETDEWEB)

Vrinat, G. [Association Francaise du Froid (AFF), 75 - Paris (France)

1997-12-31

A diagnostic methodology for refrigerating machines, equipment and plants has been defined and evaluated for EDF, the French national power utility and ADEME, the French Agency for Energy Conservation, in the framework of energy conservation objectives: the diagnostic method should enable to identify malfunctions, assess the cost efficiency of the equipment, identify limiting factors, and consider corrective measures

The bases for the differences in the training methodology for male and female athletes

Directory of Open Access Journals (Sweden)

Владимир Платонов

2017-09-01

Full Text Available The analytical review article presents the data reflecting the need for significant differentiation of the methodology of sports training for male and female athletes, which unfortunately is ignored in sports practice and is not adequately reflected in the vast majority of publications in the field of theory and methodology of sports training. This differentiation can be attributed to the following main components: the physique, strength qualities and flexibility; the energy systems; the peculiarities of the psyche and behavioral reactions; the menstrual cycle; female athlete triad; hyperandrogenism; pregnancy and parturition; and age dependence of sports performance. The clearly insufficient consideration of the peculiarities of the female body not only does not allow to fully use the natural talent of athletes for achieving the highest attainable sports performance, but also may with high probability disturb the normal age-related development and produce serious health problems in female athletes.

Security Pricing as an Enabler of Cyber-Insurance: A First Look at Differentiated Pricing Markets

OpenAIRE

Pal, Ranjan; Golubchik, Leana; Psounis, Konstantinos; Hui, Pan

2016-01-01

Despite the promising potential of network risk management services (e.g., cyber-insurance) to improve information security, their deployment is relatively scarce, primarily due to such service companies being unable to guarantee profitability. As a novel approach to making cyber-insurance services more viable, we explore a symbiotic relationship between security vendors (e.g., Symantec) capable of price differentiating their clients, and cyber-insurance agencies having possession of informat...

METHODOLOGY OF PROFESSIONAL PEDAGOGICAL EDUCATION: THEORY AND PRACTICE (theoretical and methodological foundations of vocational teacher education

Directory of Open Access Journals (Sweden)

Evgeny M. Dorozhkin

2014-01-01

Full Text Available The study is aimed at investigating a justification of the new approach to the problem of vocational education development through the prism of interdependence research methodology and practice. This conceptual setup allows determining the main directions for teacher training modernization of vocational schools. The authors note that the current socio-economic situation in our country has actualized the problem of personnel training. Politicians, economists and scientists’ speeches are all about the shortage of skilled personnel. They see the main reason of this catastrophic situation in the present system of primary and secondary vocational education. At least they concern over the current practice of pedagogical personnel training of vocational education who are to restore the system of vocational education. Our country, Russia has a great positive experience in solving this problem. Scientific-methodological centre for vocational teacher education is the Russian State Vocational Pedagogical University under the scientific direction of Academician of the Russian Academy of Education, G. M. Romantsev. The reflection of scientifictheoretical bases of this education led the authors to the analysis and designing (formation of existent and new professional and pedagogical methodology. Methods. The fundamental position of A. M. Novikov on the generality of the research (scientific and practical activity methodology has become the theoretical platform of the present study. Conceptual field, conceptual statements and professional model are presented as the whole system (or integrating factor. The theoretical framework has determined the logic of the study and its results. Scientific and educational methodology differentiation in terms of the subject of cognitive activity has allowed identifying the main scientific and practical disciplines of vocational teacher education. The creative concept as the subject ground is instrumental analysis of

The BOXES Methodology Black Box Dynamic Control

CERN Document Server

Russell, David W

2012-01-01

Robust control mechanisms customarily require knowledge of the system’s describing equations which may be of the high order differential type.  In order to produce these equations, mathematical models can often be derived and correlated with measured dynamic behavior.  There are two flaws in this approach one is the level of inexactness introduced by linearizations and the other when no model is apparent.  Several years ago a new genre of control systems came to light that are much less dependent on differential models such as fuzzy logic and genetic algorithms. Both of these soft computing solutions require quite considerable a priori system knowledge to create a control scheme and sometimes complicated training program before they can be implemented in a real world dynamic system. Michie and Chambers’ BOXES methodology created a black box system that was designed to control a mechanically unstable system with very little a priori system knowledge, linearization or approximation.  All the method need...

Changing Methodologies in Financial Audit and Their Impact on Information Systems Audit

Directory of Open Access Journals (Sweden)

Daniel VILSANOIU

2010-01-01

Full Text Available This paper tries to provide a better understanding of the relation between financial audit and information systems audit and to assess the influence the change in financial audit methodologies had on IS audit. We concluded that the COSO Internal Control – Integrated Framework was the starting point for fundamental changes in both financial and IS audit and that the Sarbanes-Oxley Act should be viewed as an enabler rather than an enforcer in establishing strong governance models. Finally, our research suggests that there is a direct causality effect between the employment of BRA (business risk audit methodologies and the growing importance of IS audit.

EnableATIS strategy assessment.

Science.gov (United States)

2014-02-01

Enabling Advanced Traveler Information Systems (EnableATIS) is the traveler information component of the Dynamic Mobility Application (DMA) program. The objective of : the EnableATIS effort is to foster transformative traveler information application...

Differential scanning microcalorimetry of intrinsically disordered proteins.

Science.gov (United States)

Permyakov, Sergei E

2012-01-01

Ultrasensitive differential scanning calorimetry (DSC) is an indispensable thermophysical technique enabling to get direct information on enthalpies accompanying heating/cooling of dilute biopolymer solutions. The thermal dependence of protein heat capacity extracted from DSC data is a valuable source of information on intrinsic disorder level of a protein. Application details and limitations of DSC technique in exploration of protein intrinsic disorder are described.

Use of programme theory to understand the differential effects of interventions across socio-economic groups in systematic reviews-a systematic methodology review.

Science.gov (United States)

Maden, Michelle; Cunliffe, Alex; McMahon, Naoimh; Booth, Andrew; Carey, Gina Michelle; Paisley, Suzy; Dickson, Rumona; Gabbay, Mark

2017-12-29

Systematic review guidance recommends the use of programme theory to inform considerations of if and how healthcare interventions may work differently across socio-economic status (SES) groups. This study aimed to address the lack of detail on how reviewers operationalise this in practice. A methodological systematic review was undertaken to assess if, how and the extent to which systematic reviewers operationalise the guidance on the use of programme theory in considerations of socio-economic inequalities in health. Multiple databases were searched from January 2013 to May 2016. Studies were included if they were systematic reviews assessing the effectiveness of an intervention and included data on SES. Two reviewers independently screened all studies, undertook quality assessment and extracted data. A narrative approach to synthesis was adopted. A total of 37 systematic reviews were included, 10 of which were explicit in the use of terminology for 'programme theory'. Twenty-nine studies used programme theory to inform both their a priori assumptions and explain their review findings. Of these, 22 incorporated considerations of both what and how interventions do/do not work in SES groups to both predict and explain their review findings. Thirteen studies acknowledged 24 unique theoretical references to support their assumptions of what or how interventions may have different effects in SES groups. Most reviewers used supplementary evidence to support their considerations of differential effectiveness. The majority of authors outlined a programme theory in the "Introduction" and "Discussion" sections of the review to inform their assumptions or provide explanations of what or how interventions may result in differential effects within or across SES groups. About a third of reviews used programme theory to inform the review analysis and/or synthesis. Few authors used programme theory to inform their inclusion criteria, data extraction or quality assessment. Twenty

High Weak Order Methods for Stochastic Differential Equations Based on Modified Equations

KAUST Repository

Abdulle, Assyr; Cohen, David; Vilmart, Gilles; Zygalakis, Konstantinos C.

2012-01-01

© 2012 Society for Industrial and Applied Mathematics. Inspired by recent advances in the theory of modified differential equations, we propose a new methodology for constructing numerical integrators with high weak order for the time integration

Current status and future prospects for enabling chemistry technology in the drug discovery process

Science.gov (United States)

Djuric, Stevan W.; Hutchins, Charles W.; Talaty, Nari N.

2016-01-01

This review covers recent advances in the implementation of enabling chemistry technologies into the drug discovery process. Areas covered include parallel synthesis chemistry, high-throughput experimentation, automated synthesis and purification methods, flow chemistry methodology including photochemistry, electrochemistry, and the handling of “dangerous” reagents. Also featured are advances in the “computer-assisted drug design” area and the expanding application of novel mass spectrometry-based techniques to a wide range of drug discovery activities. PMID:27781094

Methodological foundations of evaluation of effectiveness indicators of small-scale business activities

Directory of Open Access Journals (Sweden)

Ivanova T.

2013-01-01

Full Text Available The methodological approach to the measurement of financial indicators of small-scale enterprises has been developed. It enables to secure the comparability of financial condition indicators and the results of small-scale enterprise activities, and also to develop the methods of vertical integral estimate calculation at separate aspects of financial condition and the results of smallscale enterprise activities.

Innovation and Integrity in Intervention Research: Conceptual Issues, Methodology, and Knowledge Translation.

Science.gov (United States)

Malti, Tina; Beelmann, Andreas; Noam, Gil G; Sommer, Simon

2018-04-01

In this article, we introduce the special issue entitled Innovation and Integrity in Intervention Science. Its focus is on essential problems and prospects for intervention research examining two related topics, i.e., methodological issues and research integrity, and challenges in the transfer of research knowledge into practice and policy. The main aims are to identify how to advance methodology in order to improve research quality, examine scientific integrity in the field of intervention science, and discuss future steps to enhance the transfer of knowledge about evidence-based intervention principles into sustained practice, routine activities, and policy decisions. Themes of the special issue are twofold. The first includes questions about research methodology in intervention science, both in terms of research design and methods, as well as data analyses and the reporting of findings. Second, the issue tackles questions surrounding the types of knowledge translation frameworks that might be beneficial to mobilize the transfer of research-based knowledge into practice and public policies. The issue argues that innovations in methodology and thoughtful approaches to knowledge translation can enable transparency, quality, and sustainability of intervention research.

Background for spatial differentiation in life cycle impact assessment. The EDIP2003 methodology

DEFF Research Database (Denmark)

Potting, José; Hauschild, Michael Zwicky

2004-01-01

The code of practice of the Society of Environmental Toxicology and Chemistry and the recent international standards and technical reports from ISO are widely accepted as general frameworks for Life Cycle Assessment (LCA) but they are not detailed methodological references, since international...... between modelled impact and the occurrence of actual impact. This technical report aims to contribute to a solution of the poor accuracy of the assessed impact in typical LCA resulting from the present disregard of spatial information in LCA....

Toward genome-enabled mycology.

Science.gov (United States)

Hibbett, David S; Stajich, Jason E; Spatafora, Joseph W

2013-01-01

Genome-enabled mycology is a rapidly expanding field that is characterized by the pervasive use of genome-scale data and associated computational tools in all aspects of fungal biology. Genome-enabled mycology is integrative and often requires teams of researchers with diverse skills in organismal mycology, bioinformatics and molecular biology. This issue of Mycologia presents the first complete fungal genomes in the history of the journal, reflecting the ongoing transformation of mycology into a genome-enabled science. Here, we consider the prospects for genome-enabled mycology and the technical and social challenges that will need to be overcome to grow the database of complete fungal genomes and enable all fungal biologists to make use of the new data.

Research methodological issues in evaluating herbal interventions

Directory of Open Access Journals (Sweden)

Dipika Bansal

2010-02-01

Full Text Available Dipika Bansal, Debasish Hota, Amitava ChakrabartiPostgraduate Institute of Medical Education and Research, Chandigarh, IndiaAbstract: Randomized controlled trials provide the best evidence, and is seen as the gold standard for allopathic research. Herbal therapies are not an integral part of conventional care although they are still used by patients in their health care management. These medicines need to be subjected to rigorous research to establish their effectiveness and safety. Clearly defined treatments are required and should be recorded in a manner that enables other suitably trained researchers to reproduce them reliably. Quality control of herbal products is also a prerequisite of credible clinical trials. Methodological strategies for investigating the herbal interventions and the issues regarding appropriate patient selection, randomization and blinding, placebo effects and choice of comparator, occupational standardization and the selection of appropriate study endpoints to prove efficacy are being discussed. This paper will review research options and propose some suggestions for future research design.Keywords: CAM research, herbal therapies, methodology, clinical trial

Futures Business Models for an IoT Enabled Healthcare Sector: A Causal Layered Analysis Perspective

OpenAIRE

Julius Francis Gomes; Sara Moqaddemerad

2016-01-01

Purpose: To facilitate futures business research by proposing a novel way to combine business models as a conceptual tool with futures research techniques. Design: A futures perspective is adopted to foresight business models of the Internet of Things (IoT) enabled healthcare sector by using business models as a futures business research tool. In doing so, business models is coupled with one of the most prominent foresight methodologies, Causal Layered Analysis (CLA). Qualitative analysis...

Proteinuria: The diagnostic strategy based on urine proteins differentiation

Directory of Open Access Journals (Sweden)

Stojimirović Biljana B.

2004-01-01

Full Text Available Basal glomerular membrane represents mechanical and electrical barrier for passing of the plasma proteins. Mechanical barrier is composed of cylindrical pores and filtration fissure, and negative layer charge in exterior and interior side of basal glomerular membrane, made of heparan sulphate and sialoglicoproteine, provides certain electrical barrier. Diagnostic strategy based on different serum and urine proteins enables the differentiation of various types of proteinuria. Depending on etiology of proteinuria it can be prerenal, renal and postrenal. By analyzing albumin, armicroglobulin, immunoglobulin G and armacroglobulin, together with total protein in urine, it is possible to detect and differentiate causes of prerenal, renal (glomerular, tubular, glomerulo-tubular and postrenal proteinuria. The adequate and early differentiation of proteinuria type is of an immense diagnostic and therapeutic importance.

Multivariate analysis of microarray data: differential expression and differential connection

Directory of Open Access Journals (Sweden)

Kiiveri Harri T

2011-02-01

Full Text Available Abstract Background Typical analysis of microarray data ignores the correlation between gene expression values. In this paper we present a model for microarray data which specifically allows for correlation between genes. As a result we combine gene network ideas with linear models and differential expression. Results We use sparse inverse covariance matrices and their associated graphical representation to capture the notion of gene networks. An important issue in using these models is the identification of the pattern of zeroes in the inverse covariance matrix. The limitations of existing methods for doing this are discussed and we provide a workable solution for determining the zero pattern. We then consider a method for estimating the parameters in the inverse covariance matrix which is suitable for very high dimensional matrices. We also show how to construct multivariate tests of hypotheses. These overall multivariate tests can be broken down into two components, the first one being similar to tests for differential expression and the second involving the connections between genes. Conclusion The methods in this paper enable the extraction of a wealth of information concerning the relationships between genes which can be conveniently represented in graphical form. Differentially expressed genes can be placed in the context of the gene network and places in the gene network where unusual or interesting patterns have emerged can be identified, leading to the formulation of hypotheses for future experimentation.

National Certification Methodology for the Nuclear Weapons Stockpile

International Nuclear Information System (INIS)

Goodwin, B T; Juzaitis, R J

2006-01-01

and December of 2001 and continued in 2002 have proven useful in developing the methodology, and future workshops should prove useful in further refining this framework. Each laboratory developed an approach to certification with some differences in detailed implementation. The general methodology introduces specific quantitative indicators for assessing confidence in our nuclear weapon stockpile. The quantitative indicators are based upon performance margins for key operating characteristics and components of the system, and these are compared to uncertainties in these factors. These criteria can be summarized in a quantitative metric (for each such characteristic) expressed as: (i.e., confidence in warhead performance depends upon CR significantly exceeding unity for all these characteristics). These Confidence Ratios are proposed as a basis for guiding technical and programmatic decisions on stockpile actions. This methodology already has been deployed in certifying weapons undergoing current life extension programs or component remanufacture. The overall approach is an adaptation of standard engineering practice and lends itself to rigorous, quantitative, and explicit criteria for judging the robustness of weapon system and component performance at a detailed level. There are, of course, a number of approaches for assessing these Confidence Ratios. The general certification methodology was publicly presented for the first time to a meeting of Strategic Command SAG in January 2002 and met with general approval. At that meeting, the Laboratories committed to further refine and develop the methodology through the implementation process. This paper reflects the refinement and additional development to date. There will be even further refinement at a joint laboratory workshop later in FY03. A common certification methodology enables us to engage in peer reviews and evaluate nuclear weapon systems on the basis of explicit and objective metrics. The clarity provided by

Differential membrane proteomics using 18O-labeling to identify biomarkers for cholangiocarcinoma

DEFF Research Database (Denmark)

Kristiansen, Troels Zakarias; Harsha, H C; Grønborg, Mads

2008-01-01

Quantitative proteomic methodologies allow profiling of hundreds to thousands of proteins in a high-throughput fashion. This approach is increasingly applied to cancer biomarker discovery to identify proteins that are differentially regulated in cancers. Fractionation of protein samples based...

A methodology for the characterization and diagnosis of cognitive impairments-Application to specific language impairment.

Science.gov (United States)

Oliva, Jesús; Serrano, J Ignacio; del Castillo, M Dolores; Iglesias, Angel

2014-06-01

The diagnosis of mental disorders is in most cases very difficult because of the high heterogeneity and overlap between associated cognitive impairments. Furthermore, early and individualized diagnosis is crucial. In this paper, we propose a methodology to support the individualized characterization and diagnosis of cognitive impairments. The methodology can also be used as a test platform for existing theories on the causes of the impairments. We use computational cognitive modeling to gather information on the cognitive mechanisms underlying normal and impaired behavior. We then use this information to feed machine-learning algorithms to individually characterize the impairment and to differentiate between normal and impaired behavior. We apply the methodology to the particular case of specific language impairment (SLI) in Spanish-speaking children. The proposed methodology begins by defining a task in which normal and individuals with impairment present behavioral differences. Next we build a computational cognitive model of that task and individualize it: we build a cognitive model for each participant and optimize its parameter values to fit the behavior of each participant. Finally, we use the optimized parameter values to feed different machine learning algorithms. The methodology was applied to an existing database of 48 Spanish-speaking children (24 normal and 24 SLI children) using clustering techniques for the characterization, and different classifier techniques for the diagnosis. The characterization results show three well-differentiated groups that can be associated with the three main theories on SLI. Using a leave-one-subject-out testing methodology, all the classifiers except the DT produced sensitivity, specificity and area under curve values above 90%, reaching 100% in some cases. The results show that our methodology is able to find relevant information on the underlying cognitive mechanisms and to use it appropriately to provide better

BIM-Enabled Conceptual Modelling and Representation of Building Circulation

Directory of Open Access Journals (Sweden)

Jin Kook Lee

2014-08-01

Full Text Available This paper describes how a building information modelling (BIM-based approach for building circulation enables us to change the process of building design in terms of its computational representation and processes, focusing on the conceptual modelling and representation of circulation within buildings. BIM has been designed for use by several BIM authoring tools, in particular with the widely known interoperable industry foundation classes (IFCs, which follow an object-oriented data modelling methodology. Advances in BIM authoring tools, using space objects and their relations defined in an IFC's schema, have made it possible to model, visualize and analyse circulation within buildings prior to their construction. Agent-based circulation has long been an interdisciplinary topic of research across several areas, including design computing, computer science, architectural morphology, human behaviour and environmental psychology. Such conventional approaches to building circulation are centred on navigational knowledge about built environments, and represent specific circulation paths and regulations. This paper, however, places emphasis on the use of ‘space objects’ in BIM-enabled design processes rather than on circulation agents, the latter of which are not defined in the IFCs' schemas. By introducing and reviewing some associated research and projects, this paper also surveys how such a circulation representation is applicable to the analysis of building circulation-related rules.

Enabling professional development in mental health nursing: the role of clinical leadership.

Science.gov (United States)

Ennis, G; Happell, B; Reid-Searl, K

2015-10-01

Clinical leadership is acknowledged as important to the nursing profession. While studies continue to identify its significance in contributing to positive outcomes for consumers, the role that clinical leadership has in enabling and supporting professional development in mental health nursing is poorly understood. This study utilized a grounded theory methodology to explore the characteristics clinicians consider important for clinical leadership and its significance for mental health nursing in day-to-day clinical practice. Individual face-to-face, semi-structured interviews were conducted with nurses working in mental health settings. Participants described the important role that clinical leaders play in enabling professional development of others through role modelling and clinical teaching. They describe how nurses, whom they perceive as clinical leaders, use role modelling and clinical teaching to influence the professional development of nursing staff and undergraduate nursing students. Attributes such as professionalism and honesty were seen, by participants, as enablers for clinical leaders in effectively and positively supporting the professional development of junior staff and undergraduate nurses in mental health nursing. This paper examines clinical leadership from the perspective of mental health nurses delivering care, and highlights the important role of clinical leaders in supporting professional development in mental health nursing. © 2015 John Wiley & Sons Ltd.

Culture of science: strange history of the methodological thinking in psychology.

Science.gov (United States)

Toomela, Aaro

2007-03-01

In pre-World-War-II psychology, two directions in methodological thought-the German-Austrian and North American ways-could be differentiated. After the war, the German-Austrian methodological orientation has been largely abandoned. Compared to the pre-WWII German-Austrian psychology, modern mainstream psychology is more concerned with accumulation of facts than with general theory. Furthermore, the focus on qualitative data-in addition to quantitative data-is rarely visible. Only external-physical or statistical-rather than psychological controls are taken into account in empirical studies. Fragments--rather than wholes-and relationships are studied, and single cases that contradict group data are not analyzed. Instead of complex psychological types simple trait differences are studied, and prediction is not followed by thorough analysis of the whole situation. Last (but not least), data are not systematically related to complex theory. These limits have hindered the growth of knowledge in the behavioral sciences. A new return to an updated version of the German-Austrian methodological trajectory is suggested.

Auditor differentiation, mitigating management actions and audit reporting accuracy for distressed firms

NARCIS (Netherlands)

Bruynseels, L.M.L.; Knechel, W.R.; Willekens, M.M.T.A.

2011-01-01

In this paper we examine whether there is auditor differentiation through industry specialization and audit methodology in judging the adequacy of mitigating management actions as implemented by financially distressed companies. Using a sample of U.S. companies from manufacturing industries (SIC

Portraiture: a methodology through which success and positivity can be explored and reflected.

Science.gov (United States)

Cope, Vicki; Jones, Bronwyn; Hendricks, Joyce

2015-01-01

To present an overview of portraiture. Nursing is increasingly embracing more arts-based approaches as research methodologies. Portraiture has an underlying philosophy and process that enables the illumination of real people in real settings through the 'painting' of their stories. It is a complex methodology in which the researcher attempts to illuminate meaning of personal stories and events presented as narrative. Interview data were collected from nine participants from three nursing settings. Nurses were invited to participate in an interview or conversation designed to elicit data to address the research questions; the aim was to gain insight into the nurses' common shared meanings and shared contemplations of their feelings about resilience. Criteria for inclusion in this study required that all nurse participants were English speaking, registered with the Nurses and Midwives Board of Western Australia and that each had over five years' experience in the Western Australian healthcare environment. This was to enable reflection on significant and ongoing changes within the workplace as a possible source of resilient behaviours. This is a methodological paper. Portraiture blends aesthetics and empiricism while drawing on features of narrative, case study, phenomenology and ethnography. The portraits stand as individual vignettes revealing recognisable themes and enabling patterns of experience to emerge from each topic being studied. Each portrait requires the researcher to constantly reflect on the participant's experiences by incorporating ethnographic observations, interview responses, impressionistic records, the interpretation of context, and the researcher's experiences and insights. It is a methodology that concentrates on success and positivity to immerse the reader in carefully painted and perceptive stories of success and positivity in times of adversity. This paper describes some of the hallmarks of the portraiture method, one of which is to portray

METHODOLOGY OF PROFESSIONAL PEDAGOGICAL EDUCATION: THEORY AND PRACTICE (THEORETICAL AND METHODOLOGICAL FOUNDATIONS OF VOCATIONAL TEACHER EDUCATION

Directory of Open Access Journals (Sweden)

E. M. Dorozhkin

2014-01-01

Full Text Available The study is aimed at investigating a justification of the new approach to the problem of vocational education development through the prism of interdependence research methodology and practice. This conceptual setup allows determining the main directions for teacher training modernization of vocational schools.The authors note that the current socio-economic situation in our country has actualized the problem of personnel training. Politicians, economists and scientists’ speeches are all about the shortage of skilled personnel. They see the main reason of this catastrophic situation in the present system of primary andsecondary vocational education. At least they concern over the current practice of pedagogical personnel training of vocational education who are to restore the system of vocational education. Our country, Russia has a great positive experience in solving this problem. Scientific-methodological centre for vocational teacher education is the Russian State Vocational Pedagogical University under the scientific direction of Academicianof the Russian Academy of Education, G. M. Romantsev. The reflection of scientific-theoretical bases of this education led the authors to the analysis and designing (formation of existent and new professional and pedagogical methodology. Methods. The fundamental position of A. M. Novikov on the generality of theresearch (scientific and practical activity methodology has become the theoretical platform of the present study. Conceptual field, conceptual statements and professional model are presented as the whole system (or integrating factor. The theoretical framework has determined the logic of the study and its results.Scientific and educational methodology differentiation in terms of the subject of cogni live activity has allowed identifying the main scientific and practical disciplines of vocational teacher education. The creative concept as the subject ground is instrumental

Exploration of the Moon to Enable Lunar and Planetary Science

Science.gov (United States)

Neal, C. R.

2014-12-01

The Moon represents an enabling Solar System exploration asset because of its proximity, resources, and size. Its location has facilitated robotic missions from 5 different space agencies this century. The proximity of the Moon has stimulated commercial space activity, which is critical for sustainable space exploration. Since 2000, a new view of the Moon is coming into focus, which is very different from that of the 20th century. The documented presence of volatiles on the lunar surface, coupled with mature ilmenite-rich regolith locations, represent known resources that could be used for life support on the lunar surface for extended human stays, as well as fuel for robotic and human exploration deeper into the Solar System. The Moon also represents a natural laboratory to explore the terrestrial planets and Solar System processes. For example, it is an end-member in terrestrial planetary body differentiation. Ever since the return of the first lunar samples by Apollo 11, the magma ocean concept was developed and has been applied to both Earth and Mars. Because of the small size of the Moon, planetary differentiation was halted at an early (primary?) stage. However, we still know very little about the lunar interior, despite the Apollo Lunar Surface Experiments, and to understand the structure of the Moon will require establishing a global lunar geophysical network, something Apollo did not achieve. Also, constraining the impact chronology of the Moon allows the surfaces of other terrestrial planets to be dated and the cratering history of the inner Solar System to be constrained. The Moon also represents a natural laboratory to study space weathering of airless bodies. It is apparent, then, that human and robotic missions to the Moon will enable both science and exploration. For example, the next step in resource exploration is prospecting on the surface those deposits identified from orbit to understand the yield that can be expected. Such prospecting will also

Life-cycle energy optimisation : A proposed methodology for integrating environmental considerations early in the vehicle engineering design process

OpenAIRE

O'Reilly, Ciarán J.; Göransson, Peter; Funazaki, Atsushi; Suzuki, Tetsuya; Edlund, Stefan; Gunnarsson, Cecilia; Lundow, Jan-Olov; Cerin, Pontus; Cameron, Christopher J.; Wennhage, Per; Potting, José

2016-01-01

To enable the consideration of life cycle environmental impacts in the early stages of vehicle design, a methodology using the proxy of life cycle energy is proposed in this paper. The trade-offs in energy between vehicle production, operational performance and end-of-life are formulated as a mathematical problem, and simultaneously balanced with other transport-related functionalities, and may be optimised. The methodology is illustrated through an example design study, which is deliberately...

Identifying States along the Hematopoietic Stem Cell Differentiation Hierarchy with Single Cell Specificity via Raman Spectroscopy.

Science.gov (United States)

Ilin, Yelena; Choi, Ji Sun; Harley, Brendan A C; Kraft, Mary L

2015-11-17

A major challenge for expanding specific types of hematopoietic cells ex vivo for the treatment of blood cell pathologies is identifying the combinations of cellular and matrix cues that direct hematopoietic stem cells (HSC) to self-renew or differentiate into cell populations ex vivo. Microscale screening platforms enable minimizing the number of rare HSCs required to screen the effects of numerous cues on HSC fate decisions. These platforms create a strong demand for label-free methods that accurately identify the fate decisions of individual hematopoietic cells at specific locations on the platform. We demonstrate the capacity to identify discrete cells along the HSC differentiation hierarchy via multivariate analysis of Raman spectra. Notably, cell state identification is accurate for individual cells and independent of the biophysical properties of the functionalized polyacrylamide gels upon which these cells are cultured. We report partial least-squares discriminant analysis (PLS-DA) models of single cell Raman spectra enable identifying four dissimilar hematopoietic cell populations across the HSC lineage specification. Successful discrimination was obtained for a population enriched for long-term repopulating HSCs (LT-HSCs) versus their more differentiated progeny, including closely related short-term repopulating HSCs (ST-HSCs) and fully differentiated lymphoid (B cells) and myeloid (granulocytes) cells. The lineage-specific differentiation states of cells from these four subpopulations were accurately identified independent of the stiffness of the underlying biomaterial substrate, indicating subtle spectral variations that discriminated these populations were not masked by features from the culture substrate. This approach enables identifying the lineage-specific differentiation stages of hematopoietic cells on biomaterial substrates of differing composition and may facilitate correlating hematopoietic cell fate decisions with the extrinsic cues that

Methodology for identifying boundaries of systems important to safety in CANDU nuclear power plants

International Nuclear Information System (INIS)

Therrien, S.; Komljenovic, D.; Therrien, P.; Ruest, C.; Prevost, P.; Vaillancourt, R.

2007-01-01

This paper presents a methodology developed to identify the boundaries of the systems important to safety (SIS) at the Gentilly-2 Nuclear Power Plant (NPP), Hydro-Quebec. The SIS boundaries identification considers nuclear safety only. Components that are not identified as important to safety are systematically identified as related to safety. A global assessment process such as WANO/INPO AP-913 'Equipment Reliability Process' will be needed to implement adequate changes in the management rules of those components. The paper depicts results in applying the methodology to the Shutdown Systems 1 and 2 (SDS 1, 2), and to the Emergency Core Cooling System (ECCS). This validation process enabled fine tuning the methodology, performing a better estimate of the effort required to evaluate a system, and identifying components important to safety of these systems. (author)

Differential Detector for Measuring Radiation Fields

International Nuclear Information System (INIS)

Broide, A.; Marcus, E.; Brandys, I.; Schwartz, A.; Wengrowicz, U.; Levinson, S.; Seif, R.; Sattinger, D.; Kadmon, Y.; Tal, N.

2004-01-01

In case of a nuclear accident, it is essential to determine the source of radioactive contamination in order to analyze the risk to the environment and to the population. The radiation source may be a radioactive plume on the air or an area on the ground contaminated with radionuclides. Most commercial radiation detectors measure only the radiation field intensity but are unable to differentiate between the radiation sources. Consequently, this limitation causes a real problem in analyzing the potential risk to the near-by environment, since there is no data concerning the contamination ratios in the air and on the ground and this prevents us from taking the required steps to deal with the radiation event. This work presents a GM-tube-based Differential Detector, which enables to determine the source of contamination

Gadamerian philosophical hermeneutics as a useful methodological framework for the Delphi technique

Directory of Open Access Journals (Sweden)

Diana Guzys

2015-05-01

Full Text Available In this article we aim to demonstrate how Gadamerian philosophical hermeneutics may provide a sound methodological framework for researchers using the Delphi Technique (Delphi in studies exploring health and well-being. Reporting of the use of Delphi in health and well-being research is increasing, but less attention has been given to covering its methodological underpinnings. In Delphi, a structured anonymous conversation between participants is facilitated, via an iterative survey process. Participants are specifically selected for their knowledge and experience with the topic of interest. The purpose of structuring conversation in this manner is to cultivate collective opinion and highlight areas of disagreement, using a process that minimizes the influence of group dynamics. The underlying premise is that the opinion of a collective is more useful than that of an individual. In designing our study into health literacy, Delphi aligned well with our research focus and would enable us to capture collective views. However, we were interested in the methodology that would inform our study. As researchers, we believe that methodology provides the framework and principles for a study and is integral to research integrity. In assessing the suitability of Delphi for our research purpose, we found little information about underpinning methodology. The absence of a universally recognized or consistent methodology associated with Delphi was highlighted through a scoping review we undertook to assist us in our methodological thinking. This led us to consider alternative methodologies, which might be congruent with the key principles of Delphi. We identified Gadamerian philosophical hermeneutics as a methodology that could provide a supportive framework and principles. We suggest that this methodology may be useful in health and well-being studies utilizing the Delphi method.

Gadamerian philosophical hermeneutics as a useful methodological framework for the Delphi technique.

Science.gov (United States)

Guzys, Diana; Dickson-Swift, Virginia; Kenny, Amanda; Threlkeld, Guinever

2015-01-01

In this article we aim to demonstrate how Gadamerian philosophical hermeneutics may provide a sound methodological framework for researchers using the Delphi Technique (Delphi) in studies exploring health and well-being. Reporting of the use of Delphi in health and well-being research is increasing, but less attention has been given to covering its methodological underpinnings. In Delphi, a structured anonymous conversation between participants is facilitated, via an iterative survey process. Participants are specifically selected for their knowledge and experience with the topic of interest. The purpose of structuring conversation in this manner is to cultivate collective opinion and highlight areas of disagreement, using a process that minimizes the influence of group dynamics. The underlying premise is that the opinion of a collective is more useful than that of an individual. In designing our study into health literacy, Delphi aligned well with our research focus and would enable us to capture collective views. However, we were interested in the methodology that would inform our study. As researchers, we believe that methodology provides the framework and principles for a study and is integral to research integrity. In assessing the suitability of Delphi for our research purpose, we found little information about underpinning methodology. The absence of a universally recognized or consistent methodology associated with Delphi was highlighted through a scoping review we undertook to assist us in our methodological thinking. This led us to consider alternative methodologies, which might be congruent with the key principles of Delphi. We identified Gadamerian philosophical hermeneutics as a methodology that could provide a supportive framework and principles. We suggest that this methodology may be useful in health and well-being studies utilizing the Delphi method.

Exploring the integration of the human as a flexibility factor in CPS enabled manufacturing environments: Methodology and results

OpenAIRE

Fantini, P.; Tavola, G.; Taisch, M.; Barbosa, José; Leitão, Paulo; Liu, Y.; Sayed, M.S.; Lohse, N.

2016-01-01

Cyber Physical Systems (CPS) are expected to shape the evolution of production towards the fourth industrial revolution named Industry 4.0. The increasing integration of manufacturing processes and the strengthening of the autonomous capabilities of manufacturing systems make investigating the role of humans a primary research objective in view of emerging social and demographic megatrends. Understanding how the employees can be better integrated to enable increased flexibility in manufacturi...

Harnessing the Power of Education Research Databases with the Pearl-Harvesting Methodological Framework for Information Retrieval

Science.gov (United States)

Sandieson, Robert W.; Kirkpatrick, Lori C.; Sandieson, Rachel M.; Zimmerman, Walter

2010-01-01

Digital technologies enable the storage of vast amounts of information, accessible with remarkable ease. However, along with this facility comes the challenge to find pertinent information from the volumes of nonrelevant information. The present article describes the pearl-harvesting methodological framework for information retrieval. Pearl…

Enabling School Structure, Collective Responsibility, and a Culture of Academic Optimism: Toward a Robust Model of School Performance in Taiwan

Science.gov (United States)

Wu, Jason H.; Hoy, Wayne K.; Tarter, C. John

2013-01-01

Purpose: The purpose of this research is twofold: to test a theory of academic optimism in Taiwan elementary schools and to expand the theory by adding new variables, collective responsibility and enabling school structure, to the model. Design/methodology/approach: Structural equation modeling was used to test, refine, and expand an…

Matlab differential and integral calculus

CERN Document Server

Lopez, Cesar

2014-01-01

MATLAB is a high-level language and environment for numerical computation, visualization, and programming. Using MATLAB, you can analyze data, develop algorithms, and create models and applications. The language, tools, and built-in math functions enable you to explore multiple approaches and reach a solution faster than with spreadsheets or traditional programming languages, such as C/C++ or Java. MATLAB Differential and Integral Calculus introduces you to the MATLAB language with practical hands-on instructions and results, allowing you to quickly achieve your goals. In addition to givi

An introduction to neural network methods for differential equations

CERN Document Server

Yadav, Neha; Kumar, Manoj

2015-01-01

This book introduces a variety of neural network methods for solving differential equations arising in science and engineering. The emphasis is placed on a deep understanding of the neural network techniques, which has been presented in a mostly heuristic and intuitive manner. This approach will enable the reader to understand the working, efficiency and shortcomings of each neural network technique for solving differential equations. The objective of this book is to provide the reader with a sound understanding of the foundations of neural networks, and a comprehensive introduction to neural network methods for solving differential equations together with recent developments in the techniques and their applications. The book comprises four major sections. Section I consists of a brief overview of differential equations and the relevant physical problems arising in science and engineering. Section II illustrates the history of neural networks starting from their beginnings in the 1940s through to the renewed...

Noggin and Wnt3a enable BMP4-dependent differentiation of telencephalic stem cells into GluR-agonist responsive neurons

DEFF Research Database (Denmark)

Andersson, Therese; Duckworth, Joshua K; Fritz, Nicolas

2011-01-01

levels, that in turn exerted a concentration-dependent inhibition of BMP4-mediated mesenchymal differentiation of NSCs. Instead, BMP4 exposure of NSCs induced neuronal differentiation in mesenchyme-preventing conditions, whereas treatment with recombinant noggin alone did not. Wnt signaling is known...... to be essential for the development of neurons derived from the dorsal telencephalon, and co-stimulation of NSCs with BMP4+Wnt3a resulted in a synergistic effect yielding significantly increased number of mature neurons compared to stimulation with each factor alone. Thus whereas only a subset of BMP4-induced...... neurons derived from telencephalic NSCs, responded to glutamate receptor (GluR) agonists, over 80% of BMP4+Wnt3a-induced neurons responded appropriately to GluR-agonists. Our results increase the understanding of the role for BMP4 in differentiation of telencephalic multipotent progenitors, and reveal...

On the Inclusion of Energy-Shifting Demand Response in Production Cost Models: Methodology and a Case Study

DEFF Research Database (Denmark)

O'Connell, Niamh; Hale, Elaine; Doebber, Ian

and communications, power system characteristics, regulatory environments, market structures, and business models. The work described in this report focuses on the enablement of such analysis from the production cost modeling perspective. In particular, we contribute a bottom-up methodology for modeling load...

Income differential of female labor in Southern Brazil: dual approach

Directory of Open Access Journals (Sweden)

Rita de Cassia Garcia Margonato

2014-06-01

Full Text Available This study analyzes the formation and income differential of female labor in Southern Brazil in 2002 and 2009 based on microdata from the National Sample Survey (PNAD.The methodology is to estimate the selection and wages equations using the Heckman's Sample Selection Model (1979. For the measurement of the female income differential in commerce, industry and domestic service, compared to income of women in the service sector it is applied an adaptation of the Oaxaca-Blinder Decomposition (1973 adapted by Jann (2008. It was confirmed the hypothesis that segmentation occurs in the female labor market in Southern Brazil because the income differential cannot be explained only by personal attributes (productive or not and by formal work. There are specificities in the sectors (sector effect determining the income differential of women´s income in the labor market, moreover the sector effect explained 33% of the wage differential observed in industry, also explained 29% in the commerce and 35% of the female income gaps when compared to the service sector, which is considered as in advantage.

Combining several thermal indices to generate a unique heat comfort assessment methodology

Directory of Open Access Journals (Sweden)

Wissam EL Hachem

2015-11-01

Full Text Available Purpose: The proposed methodology hopes to provide a systematic multi-disciplinary approach to assess the thermal environment while minimizing unneeded efforts. Design/methodology/approach: Different factors affect the perception of the human thermal experience: metabolic rate (biology, surrounding temperatures (heat balance and environmental factors and cognitive treatment (physiology.This paper proposes a combination of different multidisciplinary variables to generate a unique heat comfort assessment methodology. The variables at stake are physiological, biological, and environmental. Our own heat analysis is thoroughly presented and all relevant equations are described. Findings: Most companies are oblivious about potential dangers of heat stress accidents and thus about methods to monitor and prevent them. This methodology enables the company or the concerned individual to conduct a preliminary assessment with minimal wasted resources and time in unnecessary steps whilst providing a guideline for a detailed study with minimal error rates if needed. More so, thermal comfort is an integral part of sound ergonomics practices, which in turn are decisive for the success of any lean six sigma initiative. Research limitations/implications: This methodology requires several full implementations to finalize its design. Originality/value: Most used heat comfort models are inherently uncertain and tiresome to apply. An extensive literature review confirms the need for a uniform assessment methodology that combines the different thermal comfort models such as the Fanger comfort model (PMV, PPD and WGBT since high error rates coupled with tiresome calculations often hinder the thermal assessment process.

[Adult mortality differentials in Argentina].

Science.gov (United States)

Rofman, R

1994-06-01

Adult mortality differentials in Argentina are estimated and analyzed using data from the National Social Security Administration. The study of adult mortality has attracted little attention in developing countries because of the scarcity of reliable statistics and the greater importance assigned to demographic phenomena traditionally associated with development, such as infant mortality and fertility. A sample of 39,421 records of retired persons surviving as of June 30, 1988, was analyzed by age, sex, region of residence, relative amount of pension, and social security fund of membership prior to the consolidation of the system in 1967. The thirteen former funds were grouped into the five categories of government, commerce, industry, self-employed, and other, which were assumed to be proxies for the activity sector in which the individual spent his active life. The sample is not representative of the Argentine population, since it excludes the lowest and highest socioeconomic strata and overrepresents men and urban residents. It is, however, believed to be adequate for explaining mortality differentials for most of the population covered by the social security system. The study methodology was based on the technique of logistic analysis and on the use of regional model life tables developed by Coale and others. To evaluate the effect of the study variables on the probability of dying, a regression model of maximal verisimilitude was estimated. The model relates the logit of the probability of death between ages 65 and 95 to the available explanatory variables, including their possible interactions. Life tables were constructed by sex, region of residence, previous pension fund, and income. As a test of external consistency, a model including only age and sex as explanatory variables was constructed using the methodology. The results confirmed consistency between the estimated values and other published estimates. A significant conclusion of the study was that

Four-Parameter white blood cell differential counting based on light scattering measurements

NARCIS (Netherlands)

Terstappen, Leonardus Wendelinus Mathias Marie; de Grooth, B.G.; Visscher, K.; Kouterik, F.A.; Greve, Jan

1988-01-01

Measurement of the depolarized orthogonal light scattering in flow cytometry enables one to discriminate human eosinephilic granulocytes from neutrophilic granulocytes. We use this method to perform a four-parameter differential white blood cell analysis. A simple flow cytometer was built equipped

Organizational information assets classification model and security architecture methodology

Directory of Open Access Journals (Sweden)

Mostafa Tamtaji

2015-12-01

Full Text Available Today's, Organizations are exposed with huge and diversity of information and information assets that are produced in different systems shuch as KMS, financial and accounting systems, official and industrial automation sysytems and so on and protection of these information is necessary. Cloud computing is a model for enabling ubiquitous, convenient, on-demand network access to a shared pool of configurable computing resources that can be rapidly provisioned and released.several benefits of this model cuses that organization has a great trend to implementing Cloud computing. Maintaining and management of information security is the main challenges in developing and accepting of this model. In this paper, at first, according to "design science research methodology" and compatible with "design process at information systems research", a complete categorization of organizational assets, including 355 different types of information assets in 7 groups and 3 level, is presented to managers be able to plan corresponding security controls according to importance of each groups. Then, for directing of organization to architect it’s information security in cloud computing environment, appropriate methodology is presented. Presented cloud computing security architecture , resulted proposed methodology, and presented classification model according to Delphi method and expers comments discussed and verified.

A federated semantic metadata registry framework for enabling interoperability across clinical research and care domains.

Science.gov (United States)

Sinaci, A Anil; Laleci Erturkmen, Gokce B

2013-10-01

In order to enable secondary use of Electronic Health Records (EHRs) by bridging the interoperability gap between clinical care and research domains, in this paper, a unified methodology and the supporting framework is introduced which brings together the power of metadata registries (MDR) and semantic web technologies. We introduce a federated semantic metadata registry framework by extending the ISO/IEC 11179 standard, and enable integration of data element registries through Linked Open Data (LOD) principles where each Common Data Element (CDE) can be uniquely referenced, queried and processed to enable the syntactic and semantic interoperability. Each CDE and their components are maintained as LOD resources enabling semantic links with other CDEs, terminology systems and with implementation dependent content models; hence facilitating semantic search, much effective reuse and semantic interoperability across different application domains. There are several important efforts addressing the semantic interoperability in healthcare domain such as IHE DEX profile proposal, CDISC SHARE and CDISC2RDF. Our architecture complements these by providing a framework to interlink existing data element registries and repositories for multiplying their potential for semantic interoperability to a greater extent. Open source implementation of the federated semantic MDR framework presented in this paper is the core of the semantic interoperability layer of the SALUS project which enables the execution of the post marketing safety analysis studies on top of existing EHR systems. Copyright © 2013 Elsevier Inc. All rights reserved.

Oncogenic RAS enables DNA damage- and p53-dependent differentiation of acute myeloid leukemia cells in response to chemotherapy.

Directory of Open Access Journals (Sweden)

Mona Meyer

Full Text Available Acute myeloid leukemia (AML is a clonal disease originating from myeloid progenitor cells with a heterogeneous genetic background. High-dose cytarabine is used as the standard consolidation chemotherapy. Oncogenic RAS mutations are frequently observed in AML, and are associated with beneficial response to cytarabine. Why AML-patients with oncogenic RAS benefit most from high-dose cytarabine post-remission therapy is not well understood. Here we used bone marrow cells expressing a conditional MLL-ENL-ER oncogene to investigate the interaction of oncogenic RAS and chemotherapeutic agents. We show that oncogenic RAS synergizes with cytotoxic agents such as cytarabine in activation of DNA damage checkpoints, resulting in a p53-dependent genetic program that reduces clonogenicity and increases myeloid differentiation. Our data can explain the beneficial effects observed for AML patients with oncogenic RAS treated with higher dosages of cytarabine and suggest that induction of p53-dependent differentiation, e.g. by interfering with Mdm2-mediated degradation, may be a rational approach to increase cure rate in response to chemotherapy. The data also support the notion that the therapeutic success of cytotoxic drugs may depend on their ability to promote the differentiation of tumor-initiating cells.

Understanding care in the past to develop caring science of the future: a historical methodological approach.

Science.gov (United States)

Nyborg, Vibeke N; Hvalvik, Sigrun; McCormack, Brendan

2018-05-31

In this paper, we explore how the development of historical research methodologies during the last centuries can contribute to more diverse and interdisciplinary research in future caring science, especially towards a care focus that is more person-centred. The adding of a historical approach by professional historians to the theory of person-centredness and person-centred care can develop knowledge that enables a more holistic understanding of the patient and the development of the patient perspective from the past until today. Thus, the aim was to show how developments within historical methodology can help us to understand elements of care in the past to further develop caring science in future. Historical research methodologies have advocated a "history from below" perspective, and this has enabled the evolution of systematic approaches to historical research that can be explored and critically analysed. Linked with this, the development of a more social and cultural oriented understanding of historical research has enabled historians to explore and add knowledge from a broader societal perspective. By focusing on the life of ordinary people and taking social and cultural aspects into account when trying to reconstruct the past, we can get a deeper understanding of health, care and medical development. However, an interdisciplinary research focus on person-centredness and person-centred care that includes professional historians can be challenging. In this paper, we argue that a historical perspective is necessary to meet the challenges we face in future delivery of health care to all people, in all parts of society in an ever more global world. © 2018 Nordic College of Caring Science.

The Virtual Learning Commons (VLC): Enabling Co-Innovation Across Disciplines

Science.gov (United States)

Pennington, D. D.; Gandara, A.; Del Rio, N.

2014-12-01

A key challenge for scientists addressing grand-challenge problems is identifying, understanding, and integrating potentially relevant methods, models and tools that that are rapidly evolving in the informatics community. Such tools are essential for effectively integrating data and models in complex research projects, yet it is often difficult to know what tools are available and it is not easy to understand or evaluate how they might be used in a given research context. The goal of the National Science Foundation-funded Virtual Learning Commons (VLC) is to improve awareness and understanding of emerging methodologies and technologies, facilitate individual and group evaluation of these, and trace the impact of innovations within and across teams, disciplines, and communities. The VLC is a Web-based social bookmarking site designed specifically to support knowledge exchange in research communities. It is founded on well-developed models of technology adoption, diffusion of innovation, and experiential learning. The VLC makes use of Web 2.0 (Social Web) and Web 3.0 (Semantic Web) approaches. Semantic Web approaches enable discovery of potentially relevant methods, models, and tools, while Social Web approaches enable collaborative learning about their function. The VLC is under development and the first release is expected Fall 2014.

Identification of the Causative Disease of Intermittent Claudication through Walking Motion Analysis: Feature Analysis and Differentiation

Directory of Open Access Journals (Sweden)

Tetsuyou Watanabe

2014-01-01

Full Text Available Intermittent claudication is a walking symptom. Patients with intermittent claudication experience lower limb pain after walking for a short time. However, rest relieves the pain and allows the patient to walk again. Unfortunately, this symptom predominantly arises from not 1 but 2 different diseases: LSS (lumber spinal canal stenosis and PAD (peripheral arterial disease. Patients with LSS can be subdivided by the affected vertebra into 2 main groups: L4 and L5. It is clinically very important to determine whether patients with intermittent claudication suffer from PAD, L4, or L5. This paper presents a novel SVM- (support vector machine- based methodology for such discrimination/differentiation using minimally required data, simple walking motion data in the sagittal plane. We constructed a simple walking measurement system that is easy to set up and calibrate and suitable for use by nonspecialists in small spaces. We analyzed the obtained gait patterns and derived input parameters for SVM that are also visually detectable and medically meaningful/consistent differentiation features. We present a differentiation methodology utilizing an SVM classifier. Leave-one-out cross-validation of differentiation/classification by this method yielded a total accuracy of 83%.

Enabling Mobile Communications for the Needy: Affordability Methodology, and Approaches to Requalify Universal Service Measures

Directory of Open Access Journals (Sweden)

Louis-Francois PAU

2009-01-01

Full Text Available This paper links communications and media usage to social and household economics boundaries. It highlights that in present day society, communications and media are a necessity, but not always affordable, and that they furthermore open up for addictive behaviors which raise additional financial and social risks. A simple and efficient methodology compatible with state-of-the-art social and communications business statistics is developed, which produces the residual communications and media affordability budget and ultimately the value-at-risk in terms of usage and tariffs. Sensitivity analysis provides precious information on bottom-up communications and media adoption on the basis of affordability. This approach differs from the regulated but often ineffective Universal service obligation, which instead of catering for individual needs mostly addresses macro-measures helping geographical access coverage (e.g. in rural areas. It is proposed to requalify the Universal service obligations on operators into concrete measures, allowing, with unchanged funding, the needy to adopt mobile services based on their affordability constraints by bridging the gap to a standard tariff. Case data are surveyed from various countries. ICT policy recommendations are made to support widespread and socially responsible communications access.

Job characteristics and voluntary mobility in The Netherlands: Differential education and gender patterns?

NARCIS (Netherlands)

Gesthuizen, M.J.W.

2009-01-01

Purpose - The purpose of this paper is to address the impact of the subjective evaluation of job characteristics on voluntary mobility, the impact of voluntary mobility on changes in these job characteristics, and differential education and gender patterns. Design/methodology/approach - Ordered and

Integrated Data Processing Methodology for Airborne Repeat-pass Differential SAR Interferometry

Science.gov (United States)

Dou, C.; Guo, H.; Han, C.; Yue, X.; Zhao, Y.

2014-11-01

Short temporal baseline and multiple ground deformation information can be derived from the airborne differential synthetic aperture radar Interforemetry (D-InSAR). However, affected by the turbulence of the air, the aircraft would deviate from the designed flight path with high frequent vibrations and changes both in the flight trajectory and attitude. Restricted by the accuracy of the position and orientation system (POS), these high frequent deviations can not be accurately reported, which would pose great challenges in motion compensation and interferometric process. Thus, these challenges constrain its wider applications. The objective of this paper is to investigate the accurate estimation and compensation of the residual motion errors in the airborne SAR imagery and time-varying baseline errors between the diffirent data acquirations, furthermore, to explore the integration data processing theory for the airborne D-InSAR system, and thus help to accomplish the correct derivation of the ground deformation by using the airborne D-InSAR measurements.

Enabling Design

Science.gov (United States)

2009-05-21

Figure 1. Methodology in Hierarchical Context. 2 Peter Checkland , Systems Thinking, System...Joint Forces Command, 2008. Checkland , Peter. Systems Thinking, System Practice. Chichester: John Wiley & Sons, 1981. FM 6-0 Mission Command: Command

Methodology implementation for multi objective optimisation for nuclear fleet evolution scenarios

International Nuclear Information System (INIS)

Freynet, David

2016-01-01

The issue of the evolution French nuclear fleet can be considered through the study of nuclear transition scenarios. These studies are of paramount importance as their results can greatly affect the decision making process, given that they take into account industrial concerns, investments, time, and nuclear system complexity. Such studies can be performed with the COSI code (developed at the CEA/DEN), which enables the calculation of matter inventories and fluxes across the fuel cycle (nuclear reactors and associated facilities), especially when coupled with the CESAR depletion code. The studies today performed with COSI require the definition of the various scenarios' input parameters, in order to fulfil different objectives such as minimising natural uranium consumption, waste production and so on. These parameters concern the quantities and the scheduling of spent fuel destined for reprocessing, and the number, the type and the commissioning dates of deployed reactors.This work aims to develop, validate and apply an optimisation methodology coupled with COSI, in order to determine optimal nuclear transition scenarios for a multi-objective platform. Firstly, this methodology is based on the acceleration of scenario evaluation, enabling the use of optimisation methods in a reasonable time-frame. With this goal in mind, artificial neural network irradiation surrogate models are created with the URANIE platform (developed at the CEA/DEN) and are implemented within COSI. The next step in this work is to use, adapt and compare different optimisation methods, such as URANIE's genetic algorithm and particle swarm methods, in order to define a methodology suited to this type of study. This methodology development is based on an incremental approach which progressively adds objectives, constraints and decision variables to the optimisation problem definition. The variables added, which are related to reactor deployment and spent fuel reprocessing strategies, are chosen

Methodology of shooting training using modern IT techniques

Science.gov (United States)

Gudzbeler, Grzegorz; Struniawski, Jarosław

2017-08-01

Mastering, improvement, shaping and preservation of skills of safe, efficient and effective use of the firearm requires the use of relevant methodology of conducting the shooting training. However reality of police trainings does not usually allow for intensive training shooting with the use of ammunition. An alternative solution is the use of modern training technologies. Example of this is the "Virtual system of improvement tactics of intervention services responsible for security and shooting training." Introduction of stimulator to police trainings will enable complete stuff preparation to achieve its tasks, creating potential of knowledge and experience in many areas, far exceeding the capabilities of conventional training.

The differential impact of microcredit on rural livelihoods: Case study from Ethiopia

NARCIS (Netherlands)

Siyoum, A.D.; Hilhorst, D.J.M.; Pankhurst, A.S.A.

2012-01-01

This paper examines the differential impact of credit on rural Ethiopian households. Though credit is generally expected to have a positive impact on household livelihoods, this paper argues that credit affects households differently depending on wealth. Results show that credit failed to enable

Missing Kettles and Too Few Toasters: The Forecasting Methodology at Morphy Richards

OpenAIRE

Lane, D; Hughes, D

2002-01-01

Faced with problems in forecasting at Morphy Richards, this research represents an investigation into their forecasting methodology following the hypothesis that the current forecasting system was no longer sufficient to ensure guaranteed supply to customers, or to enable forward planning.\\ud \\ud The purpose of the research was to identify any requirements for change within the forecasting system and to identify the ‘best practice’ within the industry. Primary research was carried out using a...

A hanging drop culture method to study terminal erythroid differentiation.

Science.gov (United States)

Gutiérrez, Laura; Lindeboom, Fokke; Ferreira, Rita; Drissen, Roy; Grosveld, Frank; Whyatt, David; Philipsen, Sjaak

2005-10-01

To design a culture method allowing the quantitative and qualitative analysis of terminal erythroid differentiation. Primary erythroid progenitors derived either from mouse tissues or from human umbilical cord blood were differentiated using hanging drop cultures and compared to methylcellulose cultures. Cultured cells were analyzed by FACS to assess differentiation. We describe a practical culture method by adapting the previously described hanging drop culture system to conditions allowing terminal differentiation of primary erythroid progenitors. Using minimal volumes of media and small numbers of cells, we obtained quantitative terminal erythroid differentiation within two days of culture in the case of murine cells and 4 days in the case of human cells. The established methods for ex vivo culture of primary erythroid progenitors, such as methylcellulose-based burst-forming unit-erythroid (BFU-E) and colony-forming unit-erythroid (CFU-E) assays, allow the detection of committed erythroid progenitors but are of limited value to study terminal erythroid differentiation. We show that the application of hanging drop cultures is a practical alternative that, in combination with clonogenic assays, enables a comprehensive assessment of the behavior of primary erythroid cells ex vivo in the context of genetic and drug-induced perturbations.

De/colonizing methodologies in science education: rebraiding research theory-practice-ethics with Indigenous theories and theorists

Science.gov (United States)

Higgins, Marc; Kim, Eun-Ji Amy

2018-02-01

The purpose of this article is to differentially engage in the work of thinking with Indigenous theorists and theories with decolonizing science education research methodologies in mind. As a rejoinder to Tracey McMahon, Emily Griese, and DenYelle Baete Kenyon's Cultivating Native American scientists: An application of an Indigenous model to an undergraduate research experience, we extend the notion of educationally centering Indigenous processes, pedagogies, and protocols by considering methodology a site in which (neo-)colonial logics often linger. We suggest that (re)designing methodology with Indigenous theorists and theories is an important act of resistance, refusal, and resignification; we demonstrate this significance through braiding together narratives of our engagement in this task and provide insights as to what is produced or producible.

Methodology for probability of failure assessment of offshore pipelines; Metodologia qualitativa de avaliacao da probabilidade de falha de dutos rigidos submarinos estaticos

Energy Technology Data Exchange (ETDEWEB)

Pezzi Filho, Mario [PETROBRAS, Rio de Janeiro, RJ (Brazil)

2005-07-01

In this study it is presented a methodology for assessing the likelihood of failure for every failure mechanism defined for carbon steel static offshore pipelines. This methodology is aimed to comply with the Integrity Management policy established by the Company. Decision trees are used for the development of the methodology and the evaluation of the extent and the significance of these failure mechanisms. Decision trees enable also the visualization of the logical structure of algorithms which eventually will be used in risk assessment software. The benefits of the proposed methodology are presented and it is recommended that it be tested on static offshore pipelines installed in different assets for validation. (author)

Processing methods for differential analysis of LC/MS profile data

Directory of Open Access Journals (Sweden)

Orešič Matej

2005-07-01

Full Text Available Abstract Background Liquid chromatography coupled to mass spectrometry (LC/MS has been widely used in proteomics and metabolomics research. In this context, the technology has been increasingly used for differential profiling, i.e. broad screening of biomolecular components across multiple samples in order to elucidate the observed phenotypes and discover biomarkers. One of the major challenges in this domain remains development of better solutions for processing of LC/MS data. Results We present a software package MZmine that enables differential LC/MS analysis of metabolomics data. This software is a toolbox containing methods for all data processing stages preceding differential analysis: spectral filtering, peak detection, alignment and normalization. Specifically, we developed and implemented a new recursive peak search algorithm and a secondary peak picking method for improving already aligned results, as well as a normalization tool that uses multiple internal standards. Visualization tools enable comparative viewing of data across multiple samples. Peak lists can be exported into other data analysis programs. The toolbox has already been utilized in a wide range of applications. We demonstrate its utility on an example of metabolic profiling of Catharanthus roseus cell cultures. Conclusion The software is freely available under the GNU General Public License and it can be obtained from the project web page at: http://mzmine.sourceforge.net/.

Novel molecular markers differentiate Oncorhynchus mykiss (rainbow trout and steelhead) and the O. clarki (cutthroat trout) subspecies

Science.gov (United States)

Ostberg, C.O.; Rodriguez, R.J.

2002-01-01

A suite of 26 PCR-based markers was developed that differentiates rainbow (Oncorhynchus mykiss) and coastal cutthroat trout (O. clarki clarki). The markers also differentiated rainbow from other cutthroat trout subspecies (O. clarki), and several of the markers differentiated between cutthroat trout subspecies. This system has numerous positive attributes, including: nonlethal sampling, high species-specificity and products that are easily identified and scored using agarose gel electrophoresis. The methodology described for developing the markers can be applied to virtually any system in which numerous markers are desired for identifying or differentiating species or subspecies.

Differentiation of Aedes aegypti and Aedes notoscriptus (Diptera: Culicidae) eggs using scanning electron microscopy.

Science.gov (United States)

Faull, Katherine J; Williams, Craig R

2016-05-01

Aedes notoscriptus and Aedes aegypti are both peri-domestic, invasive container-breeding mosquitoes. While the two potential arboviral vectors are bionomically similar, their sympatric distribution in Australia is limited. In this study, analyses of Ae. aegypti and Ae. notoscriptus eggs were enabled using scanning electron microscopy. Significant variations in egg length to width ratio and outer chorionic cell field morphology between Ae. aegypti and Ae. notoscriptus enabled distinction of the two species. Intraspecific variations in cell field morphology also enabled differentiation of the separate populations of both species, highlighting regional and global variation. Our study provides a comprehensive comparative analysis of inter- and intraspecific egg morphological and morphometric variation between two invasive container-breeding mosquitoes. The results indicate a high degree of intraspecific variation in Ae. notoscriptus egg morphology when compared to the eggs of Ae. aegypti. Comparative morphological analyses of Ae. aegypti and Ae. notoscriptus egg attributes using SEM allows differentiation of the species and may be helpful in understanding egg biology in relation to biotope of origin. Copyright © 2016 Elsevier Ltd. All rights reserved.

Does Gender-Specific Differential Item Functioning Affect the Structure in Vocational Interest Inventories?

Science.gov (United States)

Beinicke, Andrea; Pässler, Katja; Hell, Benedikt

2014-01-01

The study investigates consequences of eliminating items showing gender-specific differential item functioning (DIF) on the psychometric structure of a standard RIASEC interest inventory. Holland's hexagonal model was tested for structural invariance using a confirmatory methodological approach (confirmatory factor analysis and randomization…

The life of children in care in Denmark

DEFF Research Database (Denmark)

Warming, Hanne

2015-01-01

children’s perspectives in an ethically sound manner, it is necessary to combine the ‘voice approach’ with ethnomethodological insights and critical sociological analysis, which together enable ‘critical sociological empathy’. Second, that a methodological strategy that combines differentiated researcher...

"Found Performance": Towards a Musical Methodology for Exploring the Aesthetics of Care.

Science.gov (United States)

Wood, Stuart

2017-09-18

Concepts of performance in fine art reflect key processes in music therapy. Music therapy enables practitioners to reframe patients as performers, producing new meanings around the clinical knowledge attached to medical histories and constructs. In this paper, music therapy practices are considered in the wider context of art history, with reference to allied theories from social research. Tracing a century in art that has revised the performativity of found objects (starting with Duchamp's "Fountain"), and of found sound (crystallised by Cage's 4' 33) this paper proposes that music therapy might be a pioneer methodology of "found performance". Examples from music therapy and contemporary socially engaged art practices are brought as potential links between artistic methodologies and medical humanities research, with specific reference to notions of Aesthetics of Care.

Model-driven methodology for rapid deployment of smart spaces based on resource-oriented architectures.

Science.gov (United States)

Corredor, Iván; Bernardos, Ana M; Iglesias, Josué; Casar, José R

2012-01-01

Advances in electronics nowadays facilitate the design of smart spaces based on physical mash-ups of sensor and actuator devices. At the same time, software paradigms such as Internet of Things (IoT) and Web of Things (WoT) are motivating the creation of technology to support the development and deployment of web-enabled embedded sensor and actuator devices with two major objectives: (i) to integrate sensing and actuating functionalities into everyday objects, and (ii) to easily allow a diversity of devices to plug into the Internet. Currently, developers who are applying this Internet-oriented approach need to have solid understanding about specific platforms and web technologies. In order to alleviate this development process, this research proposes a Resource-Oriented and Ontology-Driven Development (ROOD) methodology based on the Model Driven Architecture (MDA). This methodology aims at enabling the development of smart spaces through a set of modeling tools and semantic technologies that support the definition of the smart space and the automatic generation of code at hardware level. ROOD feasibility is demonstrated by building an adaptive health monitoring service for a Smart Gym.

Model-Driven Methodology for Rapid Deployment of Smart Spaces Based on Resource-Oriented Architectures

Directory of Open Access Journals (Sweden)

José R. Casar

2012-07-01

Full Text Available Advances in electronics nowadays facilitate the design of smart spaces based on physical mash-ups of sensor and actuator devices. At the same time, software paradigms such as Internet of Things (IoT and Web of Things (WoT are motivating the creation of technology to support the development and deployment of web-enabled embedded sensor and actuator devices with two major objectives: (i to integrate sensing and actuating functionalities into everyday objects, and (ii to easily allow a diversity of devices to plug into the Internet. Currently, developers who are applying this Internet-oriented approach need to have solid understanding about specific platforms and web technologies. In order to alleviate this development process, this research proposes a Resource-Oriented and Ontology-Driven Development (ROOD methodology based on the Model Driven Architecture (MDA. This methodology aims at enabling the development of smart spaces through a set of modeling tools and semantic technologies that support the definition of the smart space and the automatic generation of code at hardware level. ROOD feasibility is demonstrated by building an adaptive health monitoring service for a Smart Gym.

A Methodology for Virtual Enterprise Management – Results from IMS 95001/Esprit 26509 Globeman21 project

DEFF Research Database (Denmark)

Tølle, Martin; Vesterager, Johan; Pedersen, Jens Dahl

2000-01-01

This paper presents results of the recently concluded IMS Globeman21 (Global Manufacturing in the 21st Century, IMS 95001/ESPRIT 26509) project. The results is the Virtual Enterprise Concept, a Virtual Enterprise Framework based upon GERAM (ISO/DIS 15704) and a Methodology for Virtual Enterprise...... management and engineering which is related to the Virtual Enterprise Framework (VEF). Applying the VEF as a basis structure for the Methodology provides a systematic approach for preparation and engineering of virtual enterprises. The Virtual Enterprise Framework opens up the prospect of combining...... experiences in the area of virtual enterprise into an integrated whole enabling researchers or practitioners to focus on subsets of the challenge related to the realisation of the virtual enterprise potentials. In this paper the identification, concept and requirements phases of the methodology are briefly...

Augmented nonlinear differentiator design and application to nonlinear uncertain systems.

Science.gov (United States)

Shao, Xingling; Liu, Jun; Li, Jie; Cao, Huiliang; Shen, Chong; Zhang, Xiaoming

2017-03-01

In this paper, an augmented nonlinear differentiator (AND) based on sigmoid function is developed to calculate the noise-less time derivative under noisy measurement condition. The essential philosophy of proposed AND in achieving high attenuation of noise effect is established by expanding the signal dynamics with extra state variable representing the integrated noisy measurement, then with the integral of measurement as input, the augmented differentiator is formulated to improve the estimation quality. The prominent advantages of the present differentiation technique are: (i) better noise suppression ability can be achieved without appreciable delay; (ii) the improved methodology can be readily extended to construct augmented high-order differentiator to obtain multiple derivatives. In addition, the convergence property and robustness performance against noises are investigated via singular perturbation theory and describing function method, respectively. Also, comparison with several classical differentiators is given to illustrate the superiority of AND in noise suppression. Finally, the robust control problems of nonlinear uncertain systems, including a numerical example and a mass spring system, are addressed to demonstrate the effectiveness of AND in precisely estimating the disturbance and providing the unavailable differential estimate to implement output feedback based controller. Copyright © 2016 ISA. Published by Elsevier Ltd. All rights reserved.

Spectral differential imaging detection of planets about nearby stars

International Nuclear Information System (INIS)

Smith, W.H.

1987-01-01

Direct ground-based optical imaging of planets in orbit about nearby stars may be accomplished by spectral differential imaging using multiple passband acoustooptic filters with a CCD. This technique provides two essential results. First, it provides a means to modulate the stellar flux reflected from a planet while leaving the flux from the star and other sources in the same field of view unmodulated. Second, spectral differential imaging enables the CCD detector to achieve a sufficiently high dynamic range to locate planets near a star in spite of an integrated brightness differential of 5 x 10 8 . Spectral differential imaging at nearby diffraction limited imaging conditions with telescope apodization can reduce the time to conduct a sensitive planetary search to a few hours in some cases. The feasibility of this idea is discussed here and shown to provide, in principle, the discrimination and sensitivity to detect a Jovian-class planet about stars at distances of about 10 parsecs. The detection of brown dwarfs is shown to be feasible as well. 31 references

Double Hopf bifurcation in delay differential equations

Directory of Open Access Journals (Sweden)

Redouane Qesmi

2014-07-01

Full Text Available The paper addresses the computation of elements of double Hopf bifurcation for retarded functional differential equations (FDEs with parameters. We present an efficient method for computing, simultaneously, the coefficients of center manifolds and normal forms, in terms of the original FDEs, associated with the double Hopf singularity up to an arbitrary order. Finally, we apply our results to a nonlinear model with periodic delay. This shows the applicability of the methodology in the study of delay models arising in either natural or technological problems.

Formation of the methodological matrix of the strategic analysis of the enterprise

Directory of Open Access Journals (Sweden)

N.H. Vygovskaya

2018-04-01

Full Text Available The article is devoted to the study of the methodological matrix of the strategic analysis of the enterprise. The aim of this article is to analyze the influence of methodological changes in the 20th century on the methodology of strategic analysis; critical assessment and generalization of scientific approaches to its methods. Evaluation of scientific works on analysis made it possible to identify such problems in the methodology of strategic analysis as the lack of consideration of the features of strategic analysis in the formation of its methods, which often leads to confusion of methods of financial (economic, thrifty analysis; failure to use the fact that the strategic analysis contains, besides the methods of analyzing the internal and external environment, the methods of forecast analysis aimed at forming the strategy for the development of the enterprise; identification of the concepts «image», «reception», «method» of analysis; multidirectionality and indistinctness of signs of classification of methods of strategic analysis; blind copying of foreign methods of application of techniques and methods of strategic analysis without taking into account the specifics of domestic economic conditions. The expediency of using the system approach in forming the methodological design of strategic analysis is proved, which will allow to combine the methodology as a science of methods (a broad approach to the methods of strategic analysis with methodology as a set of applied methods and methods of analysis (narrow approach to methodology. The use of the system approach allowed to distinguish three levels of the methodology of strategic analysis. The first and second levels of methodology correspond to the level of science, the third level – the practice. When developing the third level of special methods of strategic analysis, an approach is applied that differentiates them depending on the stages of strategic analysis (methods of the stage

Multimodal hybrid reasoning methodology for personalized wellbeing services.

Science.gov (United States)

Ali, Rahman; Afzal, Muhammad; Hussain, Maqbool; Ali, Maqbool; Siddiqi, Muhammad Hameed; Lee, Sungyoung; Ho Kang, Byeong

2016-02-01

A wellness system provides wellbeing recommendations to support experts in promoting a healthier lifestyle and inducing individuals to adopt healthy habits. Adopting physical activity effectively promotes a healthier lifestyle. A physical activity recommendation system assists users to adopt daily routines to form a best practice of life by involving themselves in healthy physical activities. Traditional physical activity recommendation systems focus on general recommendations applicable to a community of users rather than specific individuals. These recommendations are general in nature and are fit for the community at a certain level, but they are not relevant to every individual based on specific requirements and personal interests. To cover this aspect, we propose a multimodal hybrid reasoning methodology (HRM) that generates personalized physical activity recommendations according to the user׳s specific needs and personal interests. The methodology integrates the rule-based reasoning (RBR), case-based reasoning (CBR), and preference-based reasoning (PBR) approaches in a linear combination that enables personalization of recommendations. RBR uses explicit knowledge rules from physical activity guidelines, CBR uses implicit knowledge from experts׳ past experiences, and PBR uses users׳ personal interests and preferences. To validate the methodology, a weight management scenario is considered and experimented with. The RBR part of the methodology generates goal, weight status, and plan recommendations, the CBR part suggests the top three relevant physical activities for executing the recommended plan, and the PBR part filters out irrelevant recommendations from the suggested ones using the user׳s personal preferences and interests. To evaluate the methodology, a baseline-RBR system is developed, which is improved first using ranged rules and ultimately using a hybrid-CBR. A comparison of the results of these systems shows that hybrid-CBR outperforms the

A software methodology for compiling quantum programs

Science.gov (United States)

Häner, Thomas; Steiger, Damian S.; Svore, Krysta; Troyer, Matthias

2018-04-01

Quantum computers promise to transform our notions of computation by offering a completely new paradigm. To achieve scalable quantum computation, optimizing compilers and a corresponding software design flow will be essential. We present a software architecture for compiling quantum programs from a high-level language program to hardware-specific instructions. We describe the necessary layers of abstraction and their differences and similarities to classical layers of a computer-aided design flow. For each layer of the stack, we discuss the underlying methods for compilation and optimization. Our software methodology facilitates more rapid innovation among quantum algorithm designers, quantum hardware engineers, and experimentalists. It enables scalable compilation of complex quantum algorithms and can be targeted to any specific quantum hardware implementation.

When Playing Meets Learning: Methodological Framework for Designing Educational Games

Science.gov (United States)

Linek, Stephanie B.; Schwarz, Daniel; Bopp, Matthias; Albert, Dietrich

Game-based learning builds upon the idea of using the motivational potential of video games in the educational context. Thus, the design of educational games has to address optimizing enjoyment as well as optimizing learning. Within the EC-project ELEKTRA a methodological framework for the conceptual design of educational games was developed. Thereby state-of-the-art psycho-pedagogical approaches were combined with insights of media-psychology as well as with best-practice game design. This science-based interdisciplinary approach was enriched by enclosed empirical research to answer open questions on educational game-design. Additionally, several evaluation-cycles were implemented to achieve further improvements. The psycho-pedagogical core of the methodology can be summarized by the ELEKTRA's 4Ms: Macroadaptivity, Microadaptivity, Metacognition, and Motivation. The conceptual framework is structured in eight phases which have several interconnections and feedback-cycles that enable a close interdisciplinary collaboration between game design, pedagogy, cognitive science and media psychology.

A design methodology to reduce waste in the construction process

Institute of Scientific and Technical Information of China (English)

AndrewN.BALDWIN; SimonA.AUSTIN; AndrewKEYS

2003-01-01

This paper describes a conceptual tool to enable construction professional to identify where waste is generated during the construction of buildings and address how it can be reduced. It allows an improvement in the waste management practices on site by forecasting future waste types and volumes. It will reduce waste volumes on site through identification of wasteful design practices. The tool contributes to all stages of design and construction. At the Concept Stage of Design the proposed methodology provides a framework for reducing waste through better informed decisions. At the Detailed Design Stage it gives a methodology to address the areas of concern and provide focused information to aid the reduction of waste through informed design decisions. During construction it provides a tool to predict waste types arising on site thus allowing a system of proaclive waste management that will aid skip segregation strategies leading to improved waste recycling and waste reuse.

Job characteristics and voluntary mobility in the Netherlands : differential education and gender patterns?

NARCIS (Netherlands)

Gesthuizen, M.

2009-01-01

Purpose – The purpose of this paper is to address the impact of the subjective evaluation of job characteristics on voluntary mobility, the impact of voluntary mobility on changes in these job characteristics, and differential education and gender patterns. Design/methodology/approach – Ordered and

Energy index decomposition methodology at the plant level

Science.gov (United States)

Kumphai, Wisit

Scope and method of study. The dissertation explores the use of a high level energy intensity index as a facility-level energy performance monitoring indicator with a goal of developing a methodology for an economically based energy performance monitoring system that incorporates production information. The performance measure closely monitors energy usage, production quantity, and product mix and determines the production efficiency as a part of an ongoing process that would enable facility managers to keep track of and, in the future, be able to predict when to perform a recommissioning process. The study focuses on the use of the index decomposition methodology and explored several high level (industry, sector, and country levels) energy utilization indexes, namely, Additive Log Mean Divisia, Multiplicative Log Mean Divisia, and Additive Refined Laspeyres. One level of index decomposition is performed. The indexes are decomposed into Intensity and Product mix effects. These indexes are tested on a flow shop brick manufacturing plant model in three different climates in the United States. The indexes obtained are analyzed by fitting an ARIMA model and testing for dependency between the two decomposed indexes. Findings and conclusions. The results concluded that the Additive Refined Laspeyres index decomposition methodology is suitable to use on a flow shop, non air conditioned production environment as an energy performance monitoring indicator. It is likely that this research can be further expanded in to predicting when to perform a recommissioning process.

Web-enabling technologies for the factory floor: a web-enabling strategy for emanufacturing

Science.gov (United States)

Velez, Ricardo; Lastra, Jose L. M.; Tuokko, Reijo O.

2001-10-01

This paper is intended to address the different technologies available for Web-enabling of the factory floor. It will give an overview of the importance of Web-enabling of the factory floor, in the application of the concepts of flexible and intelligent manufacturing, in conjunction with e-commerce. As a last section, it will try to define a Web-enabling strategy for the application in eManufacturing. This is made under the scope of the electronics manufacturing industry, so every application, technology or related matter is presented under such scope.

Methodology Development and Applications of Proliferation Resistance and Physical Protection Evaluation

International Nuclear Information System (INIS)

Bari, R.A.; Peterson, P.F.; Therios, I.U.; Whitlock, J.J.

2010-01-01

We present an overview of the program on the evaluation methodology for proliferation resistance and physical protection (PR and PP) of advanced nuclear energy systems (NESs) sponsored by the Generation IV International Forum (GIF). For a proposed NES design, the methodology defines a set of challenges, analyzes system response to these challenges, and assesses outcomes. The challenges to the NES are the threats posed by potential actors (proliferant States or sub-national adversaries). The characteristics of Generation IV systems, both technical and institutional, are used to evaluate the response of the system and to determine its resistance against proliferation threats and robustness against sabotage and terrorism threats. The outcomes of the system response are expressed in terms of a set of measures, which are the high-level PR and PP characteristics of the NES. The methodology is organized to allow evaluations to be performed at the earliest stages of system design and to become more detailed and more representative as the design progresses. It can thus be used to enable a program in safeguards by design or to enhance the conceptual design process of an NES with regard to intrinsic features for PR and PP.

Methodology applied to develop the DHIE: applied methodology

CSIR Research Space (South Africa)

Herselman, Marlien

2016-12-01

Full Text Available This section will address the methodology that was applied to develop the South African Digital Health Innovation Ecosystem (DHIE). Each chapter under Section B represents a specific phase in the methodology....

Geo-Enabled, Mobile Services

DEFF Research Database (Denmark)

Jensen, Christian Søndergaard

2006-01-01

We are witnessing the emergence of a global infrastructure that enables the widespread deployment of geo-enabled, mobile services in practice. At the same time, the research community has also paid increasing attention to data management aspects of mobile services. This paper offers me...

Supply chain risk management enablers - A framework development through systematic review of the literature from 2000 to 2015

Directory of Open Access Journals (Sweden)

Kilubi, I.

2015-08-01

Full Text Available The present paper delivers a robust and systematic literature review (SLR on supply chain risk management (SCRM with the purpose to a review and analyse the literature concerning definitions and research methodologies applied, to b develop a classificatory framework which clusters existing enablers on SCRM, and to c examine the linkage between SCRM and performance. The findings reveal that not only is SCRM loosely defined, but that there are various fragmented supply chain risks enablers and that there is a strong need for a clear terminology for its building enablers. In addition to that, the review points to a lack of empirical confirmation concerning the connection between SCRM and performance. This paper contributes an overview of 80 peer-reviewed journal articles on SCRM from 2000 to the beginning of 2015. We offer an overarching definition of SCRM, synthesise and assemble the numerous enablers into preventive and responsive strategies by means of a conceptual framework. Moreover, indicating the social network theory (SNT as a potential theoretical foundation for SCRM, we further contribute to the supply chain management (SCM literature by providing propositions that guide future research.

Development of a novel set of criteria to select methodology for designing product service systems

Directory of Open Access Journals (Sweden)

Tuananh Tran

2016-04-01

Full Text Available This paper proposes eight groups of twenty nine scoring criteria that can help designers and practitioners to compare and select an appropriate methodology for a certain problem in designing product service system (PSS. PSS has been researched for more than a decade and is now becoming more and more popular in academia as well as industry. Despite that fact, the adoption of PSS is still limited for its potential. One of the main reasons is that designing PSS itself is a challenge. Designers and developers face difficulties in choosing appropriate PSS design methodologies for their projects so that they can design effective PSS offerings. By proposing eight groups of twenty nine scoring criteria, this paper enables a “step by step” process to identify the most appropriate design methodology for a company’s PSS problem. An example is also introduced to illustrate the use of the proposed scoring criteria and provide a clear picture of how different design methodologies can be utilized at their best in terms of application.

The Nordic Housing Enabler

DEFF Research Database (Denmark)

Helle, T.; Nygren, C.; Slaug, B.

2014-01-01

This study addresses development of a content-valid cross-Nordic version of the Housing Enabler and investigation of its inter-rater reliability when used in occupational therapy rating situations, involving occupational therapists, clients, and their home environments. The instrument was transla......This study addresses development of a content-valid cross-Nordic version of the Housing Enabler and investigation of its inter-rater reliability when used in occupational therapy rating situations, involving occupational therapists, clients, and their home environments. The instrument...... was translated from the original Swedish version of the Housing Enabler, and adapted according to accessibility norms and guidelines for housing design in Sweden, Denmark, Finland, and Iceland. This iterative process involved occupational therapists, architects, building engineers, and professional translators......, resulting in the Nordic Housing Enabler. For reliability testing, the sampling strategy and data collection procedures used were the same in all countries. Twenty voluntary occupational therapists, pair-wise but independently of each other, collected data from 106 cases by means of the Nordic Housing...

Methodology for attainment of density and effective atomic number through dual energy technique using microtomographic images

International Nuclear Information System (INIS)

Alves, H.; Lima, I.; Lopes, R.T.

2014-01-01

Dual energy technique for computerized microtomography shows itself as a promising method for identification of mineralogy on geological samples of heterogeneous composition. It can also assist with differentiating very similar objects regarding the attenuation coefficient, which are usually not separable during image processing and analysis of microtomographic data. Therefore, the development of a feasible and applicable methodology of dual energy in the analysis of microtomographic images was sought. - Highlights: • Dual energy technique is promising for identification of distribution of minerals. • A feasible methodology of dual energy in analysis of tomographic images was sought. • The dual energy technique is efficient for density and atomic number identification. • Simulation showed that the proposed methodology agrees with theoretical data. • Nondestructive characterization of distribution of density and chemical composition

A locomotor innovation enables water-land transition in a marine fish.

Directory of Open Access Journals (Sweden)

Shi-Tong Tonia Hsieh

Full Text Available BACKGROUND: Morphological innovations that significantly enhance performance capacity may enable exploitation of new resources and invasion of new ecological niches. The invasion of land from the aquatic realm requires dramatic structural and physiological modifications to permit survival in a gravity-dominated, aerial environment. Most fishes are obligatorily aquatic, with amphibious fishes typically making slow-moving and short forays on to land. METHODOLOGY/PRINCIPAL FINDINGS: Here I describe the behaviors and movements of a little known marine fish that moves extraordinarily rapidly on land. I found that the Pacific leaping blenny, Alticus arnoldorum, employs a tail-twisting movement on land, previously unreported in fishes. Focal point behavioral observations of Alticus show that they have largely abandoned the marine realm, feed and reproduce on land, and even defend terrestrial territories. Comparisons of these blennies' terrestrial kinematic and kinetic (i.e., force measurements with those of less terrestrial sister genera show A. arnoldorum move with greater stability and locomotor control, and can move away more rapidly from impending threats. CONCLUSIONS/SIGNIFICANCE: My results demonstrate that axial tail twisting serves as a key innovation enabling invasion of a novel marine niche. This paper highlights the potential of using this system to address general evolutionary questions about water-land transitions and niche invasions.

Organising to Enable Innovation

DEFF Research Database (Denmark)

Brink, Tove

2016-01-01

The purpose of this conceptual paper is to reveal how organising can enable innovation across organisational layers and organisational units. This approach calls for a cross-disciplinary literature review. The aim is to provide an integrated understanding of innovation in an organisational approach....... The findings reveal a continous organising process between individual/ team creativity and organisational structures/control to enable innovation at firm level. Organising provides a dynamic approach and contains the integrated reconstruction of creativity, structures and boundaries for enhanced balance...... of explorative and exploitative learning in uncertain environments. Shedding light on the cross-disciplinary theories to organise innovation provides a contribution at the firm level to enable innovation....

Hypothesis testing on the fractal structure of behavioral sequences: the Bayesian assessment of scaling methodology.

Science.gov (United States)

Moscoso del Prado Martín, Fermín

2013-12-01

I introduce the Bayesian assessment of scaling (BAS), a simple but powerful Bayesian hypothesis contrast methodology that can be used to test hypotheses on the scaling regime exhibited by a sequence of behavioral data. Rather than comparing parametric models, as typically done in previous approaches, the BAS offers a direct, nonparametric way to test whether a time series exhibits fractal scaling. The BAS provides a simpler and faster test than do previous methods, and the code for making the required computations is provided. The method also enables testing of finely specified hypotheses on the scaling indices, something that was not possible with the previously available methods. I then present 4 simulation studies showing that the BAS methodology outperforms the other methods used in the psychological literature. I conclude with a discussion of methodological issues on fractal analyses in experimental psychology. PsycINFO Database Record (c) 2014 APA, all rights reserved.

Ensuring VGI Credibility in Urban-Community Data Generation: A Methodological Research Design

Directory of Open Access Journals (Sweden)

Jamie O'Brien

2016-06-01

Full Text Available In this paper we outline the methodological development of current research into urban community formations based on combinations of qualitative (volunteered and quantitative (spatial analytical and geo-statistical data. We outline a research design that addresses problems of data quality relating to credibility in volunteered geographic information (VGI intended for Web-enabled participatory planning. Here we have drawn on a dual notion of credibility in VGI data, and propose a methodological workflow to address its criteria. We propose a ‘super-positional’ model of urban community formations, and report on the combination of quantitative and participatory methods employed to underpin its integration. The objective of this methodological phase of study is to enhance confidence in the quality of data for Web-enabled participatory planning. Our participatory method has been supported by rigorous quantification of area characteristics, including participant communities’ demographic and socio-economic contexts. This participatory method provided participants with a ready and accessible format for observing and mark-making, which allowed the investigators to iterate rapidly a system design based on participants’ responses to the workshop tasks. Participatory workshops have involved secondary school-age children in socio-economically contrasting areas of Liverpool (Merseyside, UK, which offers a test-bed for comparing communities’ formations in comparative contexts, while bringing an under-represented section of the population into a planning domain, whose experience may stem from public and non-motorised transport modalities. Data has been gathered through one-day participatory workshops, featuring questionnaire surveys, local site analysis, perception mapping and brief, textual descriptions. This innovative approach will support Web-based participation among stakeholding planners, who may benefit from well-structured, community

Role of Alternative Polyadenylation during Adipogenic Differentiation: An In Silico Approach

Science.gov (United States)

Spangenberg, Lucía; Correa, Alejandro; Dallagiovanna, Bruno; Naya, Hugo

2013-01-01

Post-transcriptional regulation of stem cell differentiation is far from being completely understood. Changes in protein levels are not fully correlated with corresponding changes in mRNAs; the observed differences might be partially explained by post-transcriptional regulation mechanisms, such as alternative polyadenylation. This would involve changes in protein binding, transcript usage, miRNAs and other non-coding RNAs. In the present work we analyzed the distribution of alternative transcripts during adipogenic differentiation and the potential role of miRNAs in post-transcriptional regulation. Our in silico analysis suggests a modest, consistent, bias in 3′UTR lengths during differentiation enabling a fine-tuned transcript regulation via small non-coding RNAs. Including these effects in the analyses partially accounts for the observed discrepancies in relative abundance of protein and mRNA. PMID:24143171

Methodology and mental illness: resistance and restorying.

Science.gov (United States)

Fisher, P; Freshwater, D

2014-04-01

Concerns with social justice have been traditionally associated with a modernist concept of the individual whose actions express an underlying, essential and unified self. This paper compares the usefulness of two methodologies (post-structuralist and narrative) that are based on a rejection of identity of a unified self and compares their usefulness in relation to the development of a social justice paradigm within mental health. It considers how professional forms of knowledge may be deconstructed by post-structural analyses, arguing that these have also been used by service users to articulate more enabling discursive alternatives. The notion of agency is central to our understanding of social justice. We question the commonly held assumption that although post-structuralism deconstructs power and challenges its legitimacy, it is nevertheless unsuited to facilitating the necessary agency to put forward viable alternatives. The second half of the paper considers how narrative research offers greater emancipatory potential by enabling the research subject to author their stories and thereby brings about their own subjective transformation. Nevertheless, the interpretation of people's stories by researchers may result in the imposition of narrative templates that erase complexities and contribute to the perpetuation of oppression. This raises ethical implications in relation to how people's stories are interpreted. © 2013 John Wiley & Sons Ltd.

Fuzzy AHP approach to prioritize enablers of green supply chain management practices: A case study of automotive component supplier

Directory of Open Access Journals (Sweden)

Himanshu Gupta

2016-07-01

Full Text Available With superlative growth in the number of small organizations over the past two decades, the impact caused to environment due to higher production output is also immense. Most governments are continuously taking measures to mitigate the risks associated with industrial growth. One such measure is to implement green practices throughout the supply chain. Since small organizations are important part of supply chain and a major contributor of industrial output they also need to implement green supply chain practices. This paper aims to find out important enablers for green supply chain management (GSCM from the view point of micro small and medium enterprises (MSMEs. Fuzzy analytical hierarchy process methodology has been used to rank the enablers, three important enablers were identified viz. top management commitment and finance, government support and regulations and recycling and reuse efforts. Managers of small organizations can benefit immensely by focusing on implementing these practices in their organizations.

Policy and methodology on NPP lifetime management in Spain

International Nuclear Information System (INIS)

Francia, L.

1998-01-01

The objectives for Spanish electricity sector are to extend the life cycle of NPPs beyond 40 years, maintaining safety and efficiency level, and to monitor and control ageing of important components to guarantee the prolonged lifetime. A plan for plant life management including licensing and regulatory aspects is proposed together with the methodology for achieving the proposed goals. The expected results are: life management integrated system for follow-up and monitoring of degradation and ageing mechanisms of important NPP components; modular system which will enable incorporation of additional components, degradation and/or performances; interrelation and support tool for other needs of NPPs (periodic safety reviews, maintenance, optimization, etc.)

Cerebral microbleeds. Imaging characteristics, causes and differential diagnoses; Zerebrale Mikroblutungen. Bildgebung, Ursachen und Differenzialdiagnosen

Energy Technology Data Exchange (ETDEWEB)

Linn, Jennifer [Universitaetsklinikum Dresden (Germany). Inst. und Poliklinik fuer Diagnostische und Interventionelle Neuroradiologie

2016-12-15

Cerebral microbleeds (CMB) represent small hemosiderin deposits, which are surrounded by normal brain tissue and can be depicted as small, rounded or ovoid areas of signal loss on susceptibility-sensitive MRI sequences. This review summarizes helpful methodological considerations to identify CMBs and to differentiate them from other causes of focal hypointensities on these sequences. Important differential diagnoses and ''mimics'' are presented and the clinical relevance of CMBs is discussed, in particular their role as important MRI marker of various cerebral small vessel diseases.

Changing Methodologies in Financial Audit and Their Impact on Information Systems Audit

OpenAIRE

Daniel VILSANOIU; Mihaela SERBAN

2010-01-01

This paper tries to provide a better understanding of the relation between financial audit and information systems audit and to assess the influence the change in financial audit methodologies had on IS audit. We concluded that the COSO Internal Control – Integrated Framework was the starting point for fundamental changes in both financial and IS audit and that the Sarbanes-Oxley Act should be viewed as an enabler rather than an enforcer in establishing strong governance models. Finally, ou...

Asteroid retrieval missions enabled by invariant manifold dynamics

Science.gov (United States)

Sánchez, Joan Pau; García Yárnoz, Daniel

2016-10-01

Near Earth Asteroids are attractive targets for new space missions; firstly, because of their scientific importance, but also because of their impact threat and prospective resources. The asteroid retrieval mission concept has thus arisen as a synergistic approach to tackle these three facets of interest in one single mission. This paper reviews the methodology used by the authors (2013) in a previous search for objects that could be transported from accessible heliocentric orbits into the Earth's neighbourhood at affordable costs (or Easily Retrievable Objects, a.k.a. EROs). This methodology consisted of a heuristic pruning and an impulsive manoeuvre trajectory optimisation. Low thrust propulsion on the other hand clearly enables the transportation of much larger objects due to its higher specific impulse. Hence, in this paper, low thrust retrieval transfers are sought using impulsive trajectories as first guesses to solve the optimal control problem. GPOPS-II is used to transcribe the continuous-time optimal control problem to a nonlinear programming problem (NLP). The latter is solved by IPOPT, an open source software package for large-scale NLPs. Finally, a natural continuation procedure that increases the asteroid mass allows to find out the largest objects that could be retrieved from a given asteroid orbit. If this retrievable mass is larger than the actual mass of the asteroid, the asteroid retrieval mission for this particular object is said to be feasible. The paper concludes with an updated list of 17 EROs, as of April 2016, with their maximum retrievable masses by means of low thrust propulsion. This ranges from 2000 tons for the easiest object to be retrieved to 300 tons for the least accessible of them.

A SURVEY OF STACKELBERG DIFFERENTIAL GAME MODELS IN SUPPLY AND MARKETING CHANNELS

Institute of Scientific and Technical Information of China (English)

Xiuli HE; Ashutosh PRASAD; Suresh P. SETHI; Genaro J. GUTIERREZ

2007-01-01

Stackelberg differential game models have been used to study sequential decision making in noncooperative games in diverse fields. In this paper, we survey recent applications of Stackelberg differential game models to the supply chain management and marketing channels literatures. A common feature of these applications is the specification of the game structure: a decentralized channel composed of a manufacturer and independent retailers, and a sequential decision procedure with demand and supply dynamics and coordination issues. In supply chain management, Stackelberg differential games have been used to investigate inventory issues, wholesale and retail pricing strategies, and outsourcing in dynamic environments. The underlying demand typically has growth dynamics or seasonal variation. In marketing, Stackelberg differential games have been used to model cooperative advertising programs, store brand and national brand advertising strategies, shelf space allocation, and pricing and advertising decisions. The demand dynamics are usually extensions of the classical advertising capital models or sales-advertising response models. We begin by explaining the Stackelberg differential game solution methodology and then provide a description of the models and results reported in the literature.

Diagnosis and differential diagnosis of Graves' orbitopathy in MRI

International Nuclear Information System (INIS)

Daubner, D.; Spieth, S.; Engellandt, K.; Kummer, R. von

2012-01-01

Imaging of Graves' orbitopathy (GO) includes radiological and nuclear medicine procedures. Depending on the method used they provide information about the distribution and activity of the disease. Magnetic resonance imaging (MRI) is not only a helpful tool for making the diagnosis it also enables differentiation of the active and inactive forms of GO due to intramuscular edema. The modality is therefore appropriate to evaluate the disease activity and the course of therapy. The disease leads to the typical enlargement of the muscle bodies of the extraocular muscles. The inferior rectus, medial rectus and levator palpebrae muscles are mostly involved. Signal changes of the intraconal and extraconal fat tissue are possible and a bilateral manifestation is common. The differential diagnosis includes inflammatory diseases and tumors, of which orbital pseudotumor (idiopathic, unspecific orbital inflammation), ocular myositis and orbital lymphoma are the most important. The specific patterns (localization, involvement of orbital structures and signal changes) can be differentiated by MRI. (orig.) [de

Factors that enable and hinder the implementation of projects in the alcohol and other drug field.

Science.gov (United States)

MacLean, Sarah; Berends, Lynda; Hunter, Barbara; Roberts, Bridget; Mugavin, Janette

2012-02-01

Few studies systematically explore elements of successful project implementation across a range of alcohol and other drug (AOD) activities. This paper provides an evidence base to inform project implementation in the AOD field. We accessed records for 127 completed projects funded by the Alcohol, Education and Rehabilitation Foundation from 2002 to 2008. An adapted realist synthesis methodology enabled us to develop categories of enablers and barriers to successful project implementation, and to identify factors statistically associated with successful project implementation, defined as meeting all funding objectives. Thematic analysis of eight case study projects allowed detailed exploration of findings. Nine enabler and 10 barrier categories were identified. Those most frequently reported as both barriers and enablers concerned partnerships with external agencies and communities, staffing and project design. Achieving supportive relationships with partner agencies and communities, employing skilled staff and implementing consumer or participant input mechanisms were statistically associated with successful project implementation. The framework described here will support development of evidence-based project funding guidelines and project performance indicators. The study provides evidence that investing project hours and resources to develop robust relationships with project partners and communities, implementing mechanisms for consumer or participant input and attracting skilled staff are legitimate and important activities, not just in themselves but because they potentially influence achievement of project funding objectives. © 2012 The Authors. ANZJPH © 2012 Public Health Association of Australia.

A partial differential equation for pseudocontact shift.

Science.gov (United States)

Charnock, G T P; Kuprov, Ilya

2014-10-07

It is demonstrated that pseudocontact shift (PCS), viewed as a scalar or a tensor field in three dimensions, obeys an elliptic partial differential equation with a source term that depends on the Hessian of the unpaired electron probability density. The equation enables straightforward PCS prediction and analysis in systems with delocalized unpaired electrons, particularly for the nuclei located in their immediate vicinity. It is also shown that the probability density of the unpaired electron may be extracted, using a regularization procedure, from PCS data.

Scenario development methodologies

International Nuclear Information System (INIS)

Eng, T.; Hudson, J.; Stephansson, O.

1994-11-01

In the period 1981-1994, SKB has studied several methodologies to systematize and visualize all the features, events and processes (FEPs) that can influence a repository for radioactive waste in the future. All the work performed is based on the terminology and basic findings in the joint SKI/SKB work on scenario development presented in the SKB Technical Report 89-35. The methodologies studied are a) Event tree analysis, b) Influence diagrams and c) Rock Engineering Systems (RES) matrices. Each one of the methodologies is explained in this report as well as examples of applications. One chapter is devoted to a comparison between the two most promising methodologies, namely: Influence diagrams and the RES methodology. In conclusion a combination of parts of the Influence diagram and the RES methodology is likely to be a promising approach. 26 refs

Methodology for stereoscopic motion-picture quality assessment

Science.gov (United States)

Voronov, Alexander; Vatolin, Dmitriy; Sumin, Denis; Napadovsky, Vyacheslav; Borisov, Alexey

2013-03-01

Creating and processing stereoscopic video imposes additional quality requirements related to view synchronization. In this work we propose a set of algorithms for detecting typical stereoscopic-video problems, which appear owing to imprecise setup of capture equipment or incorrect postprocessing. We developed a methodology for analyzing the quality of S3D motion pictures and for revealing their most problematic scenes. We then processed 10 modern stereo films, including Avatar, Resident Evil: Afterlife and Hugo, and analyzed changes in S3D-film quality over the years. This work presents real examples of common artifacts (color and sharpness mismatch, vertical disparity and excessive horizontal disparity) in the motion pictures we processed, as well as possible solutions for each problem. Our results enable improved quality assessment during the filming and postproduction stages.

Futures Business Models for an IoT Enabled Healthcare Sector: A Causal Layered Analysis Perspective

Directory of Open Access Journals (Sweden)

Julius Francis Gomes

2016-12-01

Full Text Available Purpose: To facilitate futures business research by proposing a novel way to combine business models as a conceptual tool with futures research techniques. Design: A futures perspective is adopted to foresight business models of the Internet of Things (IoT enabled healthcare sector by using business models as a futures business research tool. In doing so, business models is coupled with one of the most prominent foresight methodologies, Causal Layered Analysis (CLA. Qualitative analysis provides deeper understanding of the phenomenon through the layers of CLA; litany, social causes, worldview and myth. Findings: It is di cult to predict the far future for a technology oriented sector like healthcare. This paper presents three scenarios for short-, medium- and long-term future. Based on these scenarios we also present a set of business model elements for different future time frames. This paper shows a way to combine business models with CLA, a foresight methodology; in order to apply business models in futures business research. Besides offering early results for futures business research, this study proposes a conceptual space to work with individual business models for managerial stakeholders. Originality / Value: Much research on business models has offered conceptualization of the phenomenon, innovation through business model and transformation of business models. However, existing literature does not o er much on using business model as a futures research tool. Enabled by futures thinking, we collected key business model elements and building blocks for the futures market and ana- lyzed them through the CLA framework.

Review of differentiated approaches to antiretroviral therapy distribution.

Science.gov (United States)

Davis, Nicole; Kanagat, Natasha; Sharer, Melissa; Eagan, Sabrina; Pearson, Jennifer; Amanyeiwe, Ugochukwu Ugo

2018-02-22

In response to global trends of maximizing the number of patients receiving antiretroviral therapy (ART), this review summarizes literature describing differentiated models of ART distribution at facility and community levels in order to highlight promising strategies and identify evidence gaps. Databases and gray literature were searched, yielding thirteen final articles on differentiated ART distribution models supporting stable adult patients. Of these, seven articles focused on distribution at facility level and six at community level. Findings suggest that differentiated models of ART distribution contribute to higher retention, lower attrition, and less loss to follow-up (LTFU). These models also reduced patient wait time, travel costs, and time lost from work for drug pick-up. Facility- and community-level ART distribution models have the potential to extend treatment availability, enable improved access and adherence among people living with HIV (PLHIV), and facilitate retention in treatment and care. Gaps remain in understanding the desirability of these models for PLHIV, and the need for more information the negative and positive impacts of stigma, and identifying models to reach traditionally marginalized groups such as key populations and youth. Replicating differentiated care so efforts can reach more PLHIV will be critical to scaling these approaches across varying contexts.

Conceptual Challenges of the Systemic Approach in Understanding Cell Differentiation.

Science.gov (United States)

Paldi, Andras

2018-01-01

The cells of a multicellular organism are derived from a single zygote and genetically identical. Yet, they are phenotypically very different. This difference is the result of a process commonly called cell differentiation. How the phenotypic diversity emerges during ontogenesis or regeneration is a central and intensely studied but still unresolved issue in biology. Cell biology is facing conceptual challenges that are frequently confused with methodological difficulties. How to define a cell type? What stability or change means in the context of cell differentiation and how to deal with the ubiquitous molecular variations seen in the living cells? What are the driving forces of the change? We propose to reframe the problem of cell differentiation in a systemic way by incorporating different theoretical approaches. The new conceptual framework is able to capture the insights made at different levels of cellular organization and considered previously as contradictory. It also provides a formal strategy for further experimental studies.

Enabling Parametric Optimal Ascent Trajectory Modeling During Early Phases of Design

Science.gov (United States)

Holt, James B.; Dees, Patrick D.; Diaz, Manuel J.

2015-01-01

-modal due to the interaction of various constraints. Additionally, when these obstacles are coupled with The Program to Optimize Simulated Trajectories [1] (POST), an industry standard program to optimize ascent trajectories that is difficult to use, it requires expert trajectory analysts to effectively optimize a vehicle's ascent trajectory. As it has been pointed out, the paradigm of trajectory optimization is still a very manual one because using modern computational resources on POST is still a challenging problem. The nuances and difficulties involved in correctly utilizing, and therefore automating, the program presents a large problem. In order to address these issues, the authors will discuss a methodology that has been developed. The methodology is two-fold: first, a set of heuristics will be introduced and discussed that were captured while working with expert analysts to replicate the current state-of-the-art; secondly, leveraging the power of modern computing to evaluate multiple trajectories simultaneously, and therefore, enable the exploration of the trajectory's design space early during the pre-conceptual and conceptual phases of design. When this methodology is coupled with design of experiments in order to train surrogate models, the authors were able to visualize the trajectory design space, enabling parametric optimal ascent trajectory information to be introduced with other pre-conceptual and conceptual design tools. The potential impact of this methodology's success would be a fully automated POST evaluation suite for the purpose of conceptual and preliminary design trade studies. This will enable engineers to characterize the ascent trajectory's sensitivity to design changes in an arbitrary number of dimensions and for finding settings for trajectory specific variables, which result in optimal performance for a "dialed-in" launch vehicle design. The effort described in this paper was developed for the Advanced Concepts Office [2] at NASA Marshall

Archetype modeling methodology.

Science.gov (United States)

Moner, David; Maldonado, José Alberto; Robles, Montserrat

2018-03-01

Clinical Information Models (CIMs) expressed as archetypes play an essential role in the design and development of current Electronic Health Record (EHR) information structures. Although there exist many experiences about using archetypes in the literature, a comprehensive and formal methodology for archetype modeling does not exist. Having a modeling methodology is essential to develop quality archetypes, in order to guide the development of EHR systems and to allow the semantic interoperability of health data. In this work, an archetype modeling methodology is proposed. This paper describes its phases, the inputs and outputs of each phase, and the involved participants and tools. It also includes the description of the possible strategies to organize the modeling process. The proposed methodology is inspired by existing best practices of CIMs, software and ontology development. The methodology has been applied and evaluated in regional and national EHR projects. The application of the methodology provided useful feedback and improvements, and confirmed its advantages. The conclusion of this work is that having a formal methodology for archetype development facilitates the definition and adoption of interoperable archetypes, improves their quality, and facilitates their reuse among different information systems and EHR projects. Moreover, the proposed methodology can be also a reference for CIMs development using any other formalism. Copyright © 2018 Elsevier Inc. All rights reserved.

OBEST: The Object-Based Event Scenario Tree Methodology

International Nuclear Information System (INIS)

WYSS, GREGORY D.; DURAN, FELICIA A.

2001-01-01

Event tree analysis and Monte Carlo-based discrete event simulation have been used in risk assessment studies for many years. This report details how features of these two methods can be combined with concepts from object-oriented analysis to develop a new risk assessment methodology with some of the best features of each. The resultant Object-Based Event Scenarios Tree (OBEST) methodology enables an analyst to rapidly construct realistic models for scenarios for which an a priori discovery of event ordering is either cumbersome or impossible (especially those that exhibit inconsistent or variable event ordering, which are difficult to represent in an event tree analysis). Each scenario produced by OBEST is automatically associated with a likelihood estimate because probabilistic branching is integral to the object model definition. The OBEST method uses a recursive algorithm to solve the object model and identify all possible scenarios and their associated probabilities. Since scenario likelihoods are developed directly by the solution algorithm, they need not be computed by statistical inference based on Monte Carlo observations (as required by some discrete event simulation methods). Thus, OBEST is not only much more computationally efficient than these simulation methods, but it also discovers scenarios that have extremely low probabilities as a natural analytical result--scenarios that would likely be missed by a Monte Carlo-based method. This report documents the OBEST methodology, the demonstration software that implements it, and provides example OBEST models for several different application domains, including interactions among failing interdependent infrastructure systems, circuit analysis for fire risk evaluation in nuclear power plants, and aviation safety studies

Identification of transcript regulatory patterns in cell differentiation.

Science.gov (United States)

Gusnanto, Arief; Gosling, John Paul; Pope, Christopher

2017-10-15

Studying transcript regulatory patterns in cell differentiation is critical in understanding its complex nature of the formation and function of different cell types. This is done usually by measuring gene expression at different stages of the cell differentiation. However, if the gene expression data available are only from the mature cells, we have some challenges in identifying transcript regulatory patterns that govern the cell differentiation. We propose to exploit the information of the lineage of cell differentiation in terms of correlation structure between cell types. We assume that two different cell types that are close in the lineage will exhibit many common genes that are co-expressed relative to those that are far in the lineage. Current analysis methods tend to ignore this correlation by testing for differential expression assuming some sort of independence between cell types. We employ a Bayesian approach to estimate the posterior distribution of the mean of expression in each cell type, by taking into account the cell formation path in the lineage. This enables us to infer genes that are specific in each cell type, indicating the genes are involved in directing the cell differentiation to that particular cell type. We illustrate the method using gene expression data from a study of haematopoiesis. R codes to perform the analysis are available in http://www1.maths.leeds.ac.uk/∼arief/R/CellDiff/. a.gusnanto@leeds.ac.uk. Supplementary data are available at Bioinformatics online. © The Author (2017). Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com

A methodology for quantitatively managing the bug fixing process using Mahalanobis Taguchi system

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Boby John

2015-12-01

Full Text Available The controlling of bug fixing process during the system testing phase of software development life cycle is very important for fixing all the detected bugs within the scheduled time. The presence of open bugs often delays the release of the software or result in releasing the software with compromised functionalities. These can lead to customer dissatisfaction, cost overrun and eventually the loss of market share. In this paper, the authors propose a methodology to quantitatively manage the bug fixing process during system testing. The proposed methodology identifies the critical milestones in the system testing phase which differentiates the successful projects from the unsuccessful ones using Mahalanobis Taguchi system. Then a model is developed to predict whether a project is successful or not with the bug fix progress at critical milestones as control factors. Finally the model is used to control the bug fixing process. It is found that the performance of the proposed methodology using Mahalanobis Taguchi system is superior to the models developed using other multi-dimensional pattern recognition techniques. The proposed methodology also reduces the number of control points providing the managers with more options and flexibility to utilize the bug fixing resources across system testing phase. Moreover the methodology allows the mangers to carry out mid- course corrections to bring the bug fixing process back on track so that all the detected bugs can be fixed on time. The methodology is validated with eight new projects and the results are very encouraging.

The Nordic Housing Enabler

DEFF Research Database (Denmark)

Helle, Tina; Slaug, Bjørn; Brandt, Åse

2010-01-01

This study addresses development of a content valid cross-Nordic version of the Housing Enabler and investigation of its inter-rater reliability when used in occupational therapy rating situations, involving occupational therapists, clients and their home environments. The instrument was translated...... from the original Swedish version of the Housing Enabler, and adapted according to accessibility norms and guidelines for housing design in Sweden, Denmark, Finland and Iceland. This iterative process involved occupational therapists, architects, building engineers and professional translators......, resulting in the Nordic Housing Enabler. For reliability testing, the sampling strategy and data collection procedures used were the same in all countries. Twenty voluntary occupational therapists, pair-wise but independently from each other, collected data from 106 cases by means of the Nordic Housing...

A new methodology for modeling of direct landslide costs for transportation infrastructures

Science.gov (United States)

Klose, Martin; Terhorst, Birgit

2014-05-01

The world's transportation infrastructure is at risk of landslides in many areas across the globe. A safe and affordable operation of traffic routes are the two main criteria for transportation planning in landslide-prone areas. The right balancing of these often conflicting priorities requires, amongst others, profound knowledge of the direct costs of landslide damage. These costs include capital investments for landslide repair and mitigation as well as operational expenditures for first response and maintenance works. This contribution presents a new methodology for ex post assessment of direct landslide costs for transportation infrastructures. The methodology includes tools to compile, model, and extrapolate landslide losses on different spatial scales over time. A landslide susceptibility model enables regional cost extrapolation by means of a cost figure obtained from local cost compilation for representative case study areas. On local level, cost survey is closely linked with cost modeling, a toolset for cost estimation based on landslide databases. Cost modeling uses Landslide Disaster Management Process Models (LDMMs) and cost modules to simulate and monetize cost factors for certain types of landslide damage. The landslide susceptibility model provides a regional exposure index and updates the cost figure to a cost index which describes the costs per km of traffic route at risk of landslides. Both indexes enable the regionalization of local landslide losses. The methodology is applied and tested in a cost assessment for highways in the Lower Saxon Uplands, NW Germany, in the period 1980 to 2010. The basis of this research is a regional subset of a landslide database for the Federal Republic of Germany. In the 7,000 km² large Lower Saxon Uplands, 77 km of highway are located in potential landslide hazard area. Annual average costs of 52k per km of highway at risk of landslides are identified as cost index for a local case study area in this region. The

Engaging Axiology: Enabling MeaningfulTransdisciplinary Collaboration in Adapted Physical Activity.

Science.gov (United States)

Peers, Danielle

2018-07-01

In this article, I explore the concept of axiology in the context of adapted physical activity research and analyze its connection to the more commonly discussed paradigmatic assumptions of epistemology and ontology. Following methodological scholars, I argue for an acknowledgment of the pivotal role that axiology already plays in adapted physical activity research and for the potential interdisciplinary and transdisciplinary opportunities that could be enabled by engaging with axiology in more explicit ways. I discuss a number of potential axiological gaps between the field of adapted physical activity and disability communities, arguing that such differences may undermine attempts at doing meaningful transdisciplinary research with such communities. I offer strategies for bridging these axiological gaps, encouraging us to work together in axiologically reflexive ways in order to increase meaningful opportunities for more people with disabilities to be engaged in the movement-based activities and communities of their choice.

Using social media for health research: Methodological and ethical considerations for recruitment and intervention delivery.

Science.gov (United States)

Arigo, Danielle; Pagoto, Sherry; Carter-Harris, Lisa; Lillie, Sarah E; Nebeker, Camille

2018-01-01

As the popularity and diversity of social media platforms increases so does their utility for health research. Using social media for recruitment into clinical studies and/or delivering health behavior interventions may increase reach to a broader audience. However, evidence supporting the efficacy of these approaches is limited, and key questions remain with respect to optimal benchmarks, intervention development and methodology, participant engagement, informed consent, privacy, and data management. Little methodological guidance is available to researchers interested in using social media for health research. In this Tutorial, we summarize the content of the 2017 Society for Behavioral Medicine Pre-Conference Course entitled 'Using Social Media for Research,' at which the authors presented their experiences with methodological and ethical issues relating to social media-enabled research recruitment and intervention delivery. We identify common pitfalls and provide recommendations for recruitment and intervention via social media. We also discuss the ethical and responsible conduct of research using social media for each of these purposes.

Differentiating for Multiple Intelligences: A Study of Students' Understandings through the Use of Aesthetic Representations

Science.gov (United States)

Crim, Courtney L.; Kennedy, Kimberley D.; Thornton, Jenifer S.

2013-01-01

This article reviews the relevant literature in regard to differentiation, multiple intelligences, and aesthetic representations. Next, it presents the methodology, reports findings, and discusses themes related to the authors' research questions. Finally, it concludes that tapping into students' multiple intelligence strength(s) is an excellent…

Methodology and Results of Mathematical Modelling of Complex Technological Processes

Science.gov (United States)

Mokrova, Nataliya V.

2018-03-01

The methodology of system analysis allows us to draw a mathematical model of the complex technological process. The mathematical description of the plasma-chemical process was proposed. The importance the quenching rate and initial temperature decrease time was confirmed for producing the maximum amount of the target product. The results of numerical integration of the system of differential equations can be used to describe reagent concentrations, plasma jet rate and temperature in order to achieve optimal mode of hardening. Such models are applicable both for solving control problems and predicting future states of sophisticated technological systems.

Work-Related Stress Risk Assessment in Italy: A Methodological Proposal Adapted to Regulatory Guidelines

Directory of Open Access Journals (Sweden)

Benedetta Persechino

2013-06-01

Conclusion: This methodological proposal is new on the Italian work-related stress risk assessment scene. Besides providing an evaluation approach using scientifically validated instruments, it ensures the active participation of occupational health professionals in each company. The assessment tools provided enable companies not only to comply with the law, but also to contribute to a database for monitoring and assessment and give access to a reserved area for data analysis and comparisons.

MinePath: Mining for Phenotype Differential Sub-paths in Molecular Pathways

Science.gov (United States)

Koumakis, Lefteris; Kartsaki, Evgenia; Chatzimina, Maria; Zervakis, Michalis; Vassou, Despoina; Marias, Kostas; Moustakis, Vassilis; Potamias, George

2016-01-01

Pathway analysis methodologies couple traditional gene expression analysis with knowledge encoded in established molecular pathway networks, offering a promising approach towards the biological interpretation of phenotype differentiating genes. Early pathway analysis methodologies, named as gene set analysis (GSA), view pathways just as plain lists of genes without taking into account either the underlying pathway network topology or the involved gene regulatory relations. These approaches, even if they achieve computational efficiency and simplicity, consider pathways that involve the same genes as equivalent in terms of their gene enrichment characteristics. Most recent pathway analysis approaches take into account the underlying gene regulatory relations by examining their consistency with gene expression profiles and computing a score for each profile. Even with this approach, assessing and scoring single-relations limits the ability to reveal key gene regulation mechanisms hidden in longer pathway sub-paths. We introduce MinePath, a pathway analysis methodology that addresses and overcomes the aforementioned problems. MinePath facilitates the decomposition of pathways into their constituent sub-paths. Decomposition leads to the transformation of single-relations to complex regulation sub-paths. Regulation sub-paths are then matched with gene expression sample profiles in order to evaluate their functional status and to assess phenotype differential power. Assessment of differential power supports the identification of the most discriminant profiles. In addition, MinePath assess the significance of the pathways as a whole, ranking them by their p-values. Comparison results with state-of-the-art pathway analysis systems are indicative for the soundness and reliability of the MinePath approach. In contrast with many pathway analysis tools, MinePath is a web-based system (www.minepath.org) offering dynamic and rich pathway visualization functionality, with the

Refined Fuchs inequalities for systems of linear differential equations

International Nuclear Information System (INIS)

Gontsov, R R

2004-01-01

We refine the Fuchs inequalities obtained by Corel for systems of linear meromorphic differential equations given on the Riemann sphere. Fuchs inequalities enable one to estimate the sum of exponents of the system over all its singular points. We refine these well-known inequalities by considering the Jordan structure of the leading coefficient of the Laurent series for the matrix of the right-hand side of the system in the neighbourhood of a singular point

The value of electrocardiography for differential diagnosis in wide QRS complex tachycardia.

Science.gov (United States)

Sousa, Pedro A; Pereira, Salomé; Candeias, Rui; de Jesus, Ilídio

2014-03-01

Correct diagnosis in wide QRS complex tachycardia remains a challenge. Differential diagnosis between ventricular and supraventricular tachycardia has important therapeutic and prognostic implications, and although data from clinical history and physical examination may suggest a particular origin, it is the 12-lead surface electrocardiogram that usually enables this differentiation. Since 1978, various electrocardiographic criteria have been proposed for the differential diagnosis of wide complex tachycardias, particularly the presence of atrioventricular dissociation, and the axis, duration and morphology of QRS complexes. Despite the wide variety of criteria, diagnosis is still often difficult, and errors can have serious consequences. To reduce such errors, several differential diagnosis algorithms have been proposed since 1991. However, in a small percentage of wide QRS tachycardias the diagnosis remains uncertain and in these the wisest decision is to treat them as ventricular tachycardias. The authors' objective was to review the main electrocardiographic criteria and differential diagnosis algorithms of wide QRS tachycardia. Copyright © 2012 Sociedade Portuguesa de Cardiologia. Published by Elsevier España. All rights reserved.

Advanced-Retarded Differential Equations in Quantum Photonic Systems

Science.gov (United States)

Alvarez-Rodriguez, Unai; Perez-Leija, Armando; Egusquiza, Iñigo L.; Gräfe, Markus; Sanz, Mikel; Lamata, Lucas; Szameit, Alexander; Solano, Enrique

2017-01-01

We propose the realization of photonic circuits whose dynamics is governed by advanced-retarded differential equations. Beyond their mathematical interest, these photonic configurations enable the implementation of quantum feedback and feedforward without requiring any intermediate measurement. We show how this protocol can be applied to implement interesting delay effects in the quantum regime, as well as in the classical limit. Our results elucidate the potential of the protocol as a promising route towards integrated quantum control systems on a chip. PMID:28230090

Engendering Development: Some Methodological Perspectives on Child Labour

Directory of Open Access Journals (Sweden)

Erica Burman

2006-01-01

Full Text Available In this article I address when and why it is useful to focus on gender in the design and conceptualisation of developmental psychological research. Since methodological debates treated in the abstract tend to lack both the specificity and rigour that application to a particular context or topic imports, I take a particular focus for my discussion: child labour. In doing so I hope to highlight the analytical and practical gains of bringing gendered agendas alongside, and into, developmental research. While child labour may seem a rather curious topic for discussion of developmental psychological research practice, this article will show how it indicates with particular clarity issues that mainstream psychological research often occludes or forgets. In particular, I explore analytical and methodological benefits of exploring the diverse ways gender structures notions of childhood, alongside the developmental commonalities and asymmetries of gender and age as categories. I suggest that the usual assumed elision between women and children is often unhelpful for both women and children. Instead, an analytical attention to the shifting forms and relations of children's work facilitates more differentiated perspectives on how its meanings reflect economic and cultural (including gendered conditions, and so attends better to social inequalities. These inequalities also structure the methodological conditions and paradigms for research with children, and so the article finishes by elaborating from this discussion of child labour four key principles for engendering psychological research with and about children, which also have broader implications for conceptualisations of the relations between gender, childhood, culture and families. URN: urn:nbn:de:0114-fqs060111

Three-dimensional printed knotted reactors enabling highly sensitive differentiation of silver nanoparticles and ions in aqueous environmental samples

International Nuclear Information System (INIS)

Su, Cheng-Kuan; Hsieh, Meng-Hsuan; Sun, Yuh-Chang

2016-01-01

Whether silver nanoparticles (AgNPs) persist or release silver ions (Ag + ) when discharged into a natural environment has remained an unresolved issue. In this study, we employed a low-cost stereolithographic three-dimensional printing (3DP) technology to fabricate the angle-defined knotted reactors (KRs) to construct a simple differentiation scheme for quantitative assessment of Ag + ions and AgNPs in municipal wastewater samples. We chose xanthan/phosphate-buffered saline as a dispersion medium for in situ stabilization of the two silver species, while also facilitating their extraction from complicated wastewater matrices. After method optimization, we measured extraction efficiencies of 54.5 and 32.3% for retaining Ag + ions and AgNPs, respectively, in the printed KR (768-turn), with detection limits (DLs) of 0.86 and 0.52 ng L −1 when determining Ag + ions and AgNPs, respectively (sample run at pH 11 without a rinse solution), and 0.86 ng L −1 when determining Ag + ions alone (sample run at pH 12 with a 1.5-mL rinse solution). The proposed scheme is tolerant of the wastewater matrix and provides more reliable differentiation between Ag + /AgNPs than does a conventional filtration method. The concept and applicability of adopting 3DP technology to renew traditional KR devices were evidently proven by means of these significantly improved analytical performance. Our analytical data suggested that the concentrations of Ag + ions and AgNPs in the tested industrial wastewater sample were both higher than those in domestic wastewater, implying that industrial activity might be a main source of environmental silver species, rather than domestic discharge from AgNP-containing products. - Highlights: • 3D printed knotted reactors are utilized to differentiate AgNPs and Ag + ions. • Xanthan/phosphate-buffered saline is used for stabilizing the two silver species. • Extraction efficiency up to 54.5% is available for retaining Ag + ion species. • The

Differential InP HEMT MMIC Amplifiers Embedded in Waveguides

Science.gov (United States)

Kangaslahti, Pekka; Schlecht, Erich; Samoska, Lorene

2009-01-01

Monolithic microwave integrated-circuit (MMIC) amplifiers of a type now being developed for operation at frequencies of hundreds of gigahertz contain InP high-electron-mobility transistors (HEMTs) in a differential configuration. The differential configuration makes it possible to obtain gains greater than those of amplifiers having the single-ended configuration. To reduce losses associated with packaging, the MMIC chips are designed integrally with, and embedded in, waveguide packages, with the additional benefit that the packages are compact enough to fit into phased transmitting and/or receiving antenna arrays. Differential configurations (which are inherently balanced) have been used to extend the upper limits of operating frequencies of complementary metal oxide/semiconductor (CMOS) amplifiers to the microwave range but, until now, have not been applied in millimeter- wave amplifier circuits. Baluns have traditionally been used to transform from single-ended to balanced configurations, but baluns tend to be lossy. Instead of baluns, finlines are used to effect this transformation in the present line of development. Finlines have been used extensively to drive millimeter- wave mixers in balanced configurations. In the present extension of the finline balancing concept, finline transitions are integrated onto the affected MMICs (see figure). The differential configuration creates a virtual ground within each pair of InP HEMT gate fingers, eliminating the need for inductive vias to ground. Elimination of these vias greatly reduces parasitic components of current and the associated losses within an amplifier, thereby enabling more nearly complete utilization of the full performance of each transistor. The differential configuration offers the additional benefit of multiplying (relative to the single-ended configuration) the input and output impedances of each transistor by a factor of four, so that it is possible to use large transistors that would otherwise have

Prediction of Gap Asymmetry in Differential Micro Accelerometers

Directory of Open Access Journals (Sweden)

Xiaoping He

2012-05-01

Full Text Available Gap asymmetry in differential capacitors is the primary source of the zero bias output of force-balanced micro accelerometers. It is also used to evaluate the applicability of differential structures in MEMS manufacturing. Therefore, determining the asymmetry level has considerable significance for the design of MEMS devices. This paper proposes an experimental-theoretical method for predicting gap asymmetry in differential sensing capacitors of micro accelerometers. The method involves three processes: first, bi-directional measurement, which can sharply reduce the influence of the feedback circuit on bias output, is proposed. Experiments are then carried out on a centrifuge to obtain the input and output data of an accelerometer. Second, the analytical input-output relationship of the accelerometer with gap asymmetry and circuit error is theoretically derived. Finally, the prediction methodology combines the measurement results and analytical derivation to identify the asymmetric error of 30 accelerometers fabricated by DRIE. Results indicate that the level of asymmetry induced by fabrication uncertainty is about ±5 × 10⁻², and that the absolute error is about ±0.2 µm under a 4 µm gap.

Comparing Structural Identification Methodologies for Fatigue Life Prediction of a Highway Bridge

Directory of Open Access Journals (Sweden)

Sai G. S. Pai

2018-01-01

Full Text Available Accurate measurement-data interpretation leads to increased understanding of structural behavior and enhanced asset-management decision making. In this paper, four data-interpretation methodologies, residual minimization, traditional Bayesian model updating, modified Bayesian model updating (with an L∞-norm-based Gaussian likelihood function, and error-domain model falsification (EDMF, a method that rejects models that have unlikely differences between predictions and measurements, are compared. In the modified Bayesian model updating methodology, a correction is used in the likelihood function to account for the effect of a finite number of measurements on posterior probability–density functions. The application of these data-interpretation methodologies for condition assessment and fatigue life prediction is illustrated on a highway steel–concrete composite bridge having four spans with a total length of 219 m. A detailed 3D finite-element plate and beam model of the bridge and weigh-in-motion data are used to obtain the time–stress response at a fatigue critical location along the bridge span. The time–stress response, presented as a histogram, is compared to measured strain responses either to update prior knowledge of model parameters using residual minimization and Bayesian methodologies or to obtain candidate model instances using the EDMF methodology. It is concluded that the EDMF and modified Bayesian model updating methodologies provide robust prediction of fatigue life compared with residual minimization and traditional Bayesian model updating in the presence of correlated non-Gaussian uncertainty. EDMF has additional advantages due to ease of understanding and applicability for practicing engineers, thus enabling incremental asset-management decision making over long service lives. Finally, parallel implementations of EDMF using grid sampling have lower computations times than implementations using adaptive sampling.

DIFFERENTIAL EFFECT OF NATIONAL VS. REGIONAL CELEBRITIES ON CONSUMER ATTITUDES

OpenAIRE

Varsha JAIN; Subhadip ROY; Abhishek KUMAR; Anusha KABRA

2010-01-01

The present study explores the differential effects of having a National/Regional celebrity in an advertisement/ endorsement. More specifically the study intends to find out whether a National celebrity would have a more favorable impact on consumer attitudes than a Regional celebrity when endorsing the same product. Experimental design was used as the research methodology. A 3 (National Celebrity/Regional Celebrity/No Celebrity) X 2 (High/Low Involvement Product) design was conducted on stud...

“Found Performance”: Towards a Musical Methodology for Exploring the Aesthetics of Care

Directory of Open Access Journals (Sweden)

Stuart Wood

2017-09-01

Full Text Available Concepts of performance in fine art reflect key processes in music therapy. Music therapy enables practitioners to reframe patients as performers, producing new meanings around the clinical knowledge attached to medical histories and constructs. In this paper, music therapy practices are considered in the wider context of art history, with reference to allied theories from social research. Tracing a century in art that has revised the performativity of found objects (starting with Duchamp’s “Fountain”, and of found sound (crystallised by Cage’s 4′ 33 this paper proposes that music therapy might be a pioneer methodology of “found performance”. Examples from music therapy and contemporary socially engaged art practices are brought as potential links between artistic methodologies and medical humanities research, with specific reference to notions of Aesthetics of Care.

A simple nanoindentation-based methodology to assess the strength of brittle thin films

International Nuclear Information System (INIS)

Borrero-Lopez, Oscar; Hoffman, Mark; Bendavid, Avi; Martin, Phil J.

2008-01-01

In this work, we report a simple methodology to assess the mechanical strength of sub-micron brittle films. Nanoindentation of as-deposited tetrahedral amorphous carbon (ta-C) and Ti-Si-N nanocomposite films on silicon substrates followed by cross-sectional examination of the damage with a focused ion beam (FIB) miller allows the occurrence of cracking to be assessed in comparison with discontinuities (pop-ins) in the load-displacement curves. Strength is determined from the critical loads at which first cracking occurs using the theory of plates on a soft foundation. This methodology enables Weibull plots to be readily obtained, avoiding complex freestanding-film machining processes. This is of great relevance, since the mechanical strength of thin films ultimately controls their reliable use in a broad range of functional uses such as tribological coatings, magnetic drives, MEMS and biomedical applications

RANS based CFD methodology for a real scale 217-pin wire-wrapped fuel assembly of KAERI PGSFR

Energy Technology Data Exchange (ETDEWEB)

Jeong, Jae-Ho, E-mail: jhjeong@kaeri.re.kr [Korea Atomic Energy Research Institute, 989-111 Daedeok-daero, Yuseoung-gu, Daejeon (Korea, Republic of); Song, Min-Seop [Department of Nuclear Engineering, Seoul National University, 559 Gwanak-ro, Gwanak-gu, Seoul (Korea, Republic of); Lee, Kwi-Lim [Korea Atomic Energy Research Institute, 989-111 Daedeok-daero, Yuseoung-gu, Daejeon (Korea, Republic of)

2017-03-15

Highlights: • This paper presents a suitable way for a practical RANS based CFD methodology which is applicable to real scale 217-pin wire-wrapped fuel assembly of KAERI PGSFR. • A key point of differentiation of the RANS based CFD methodology in this study is adapting an innovative grid generation method using a fortran based in-house code with a GGI function in a general-purpose commercial CFD code, CFX. • The RANS based CFD methodology is implemented with high resolution scheme and SST turbulence model in the 7-pin 37-pin, and 127-pin wire-wrapped fuel assembly of PNC and JNC. Furthermore, the RANS based CFD methodology can be successfully extended to the real scale 217-pin wire-wrapped fuel bundles of KAERI PGSFR. • Three-dimensional thermal-hydraulic characteristics have been also investigated briefly. - Abstract: This paper presents a suitable way for a practical RANS (Reynolds Averaged Navier-Stokes simulation) based CFD (Computational Fluid Dynamics) methodology which is applicable to real scale 217-pin wire-wrapped fuel assembly of KAERI (Korea Atomic Energy Research Institute) PGSFR (Prototype Gen-IV Sodium-cooled Fast Reactor). The main purpose of the current study is to support license issue for the KAERI PGSFR core safety and to elucidate thermal-hydraulic characteristics in a 217-pin wire-wrapped fuel assembly of KAERI PGSFR. A key point of differentiation of the RANS based CFD methodology in this study is adapting an innovative grid generation method using a fortran based in-house code with a GGI (General Grid Interface) function in a general-purpose commercial CFD code, CFX. The innovative grid generation method with GGI function can achieve to simulate a real wire shape with minimizing cell skewness. The RANS based CFD methodology is implemented with high resolution scheme in convection term and SST (Shear Stress Transport) turbulence model in the 7-pin 37-pin, and 127-pin wire-wrapped fuel assembly of PNC (Power reactor and Nuclear fuel

Area-specific temporal control of corticospinal motor neuron differentiation by COUP-TFI

Science.gov (United States)

Tomassy, Giulio Srubek; De Leonibus, Elvira; Jabaudon, Denis; Lodato, Simona; Alfano, Christian; Mele, Andrea; Macklis, Jeffrey D.; Studer, Michèle

2010-01-01

Transcription factors with gradients of expression in neocortical progenitors give rise to distinct motor and sensory cortical areas by controlling the area-specific differentiation of distinct neuronal subtypes. However, the molecular mechanisms underlying this area-restricted control are still unclear. Here, we show that COUP-TFI controls the timing of birth and specification of corticospinal motor neurons (CSMN) in somatosensory cortex via repression of a CSMN differentiation program. Loss of COUP-TFI function causes an area-specific premature generation of neurons with cardinal features of CSMN, which project to subcerebral structures, including the spinal cord. Concurrently, genuine CSMN differentiate imprecisely and do not project beyond the pons, together resulting in impaired skilled motor function in adult mice with cortical COUP-TFI loss-of-function. Our findings indicate that COUP-TFI exerts critical areal and temporal control over the precise differentiation of CSMN during corticogenesis, thereby enabling the area-specific functional features of motor and sensory areas to arise. PMID:20133588

On Certain New Methodology for Reducing Sensor and Readout Electronics Circuitry Noise in Digital Domain

Science.gov (United States)

Kizhner, Semion; Miko, Joseph; Bradley, Damon; Heinzen, Katherine

2008-01-01

NASA Hubble Space Telescope (HST) and upcoming cosmology science missions carry instruments with multiple focal planes populated with many large sensor detector arrays. These sensors are passively cooled to low temperatures for low-level light (L3) and near-infrared (NIR) signal detection, and the sensor readout electronics circuitry must perform at extremely low noise levels to enable new required science measurements. Because we are at the technological edge of enhanced performance for sensors and readout electronics circuitry, as determined by thermal noise level at given temperature in analog domain, we must find new ways of further compensating for the noise in the signal digital domain. To facilitate this new approach, state-of-the-art sensors are augmented at their array hardware boundaries by non-illuminated reference pixels, which can be used to reduce noise attributed to sensors. There are a few proposed methodologies of processing in the digital domain the information carried by reference pixels, as employed by the Hubble Space Telescope and the James Webb Space Telescope Projects. These methods involve using spatial and temporal statistical parameters derived from boundary reference pixel information to enhance the active (non-reference) pixel signals. To make a step beyond this heritage methodology, we apply the NASA-developed technology known as the Hilbert- Huang Transform Data Processing System (HHT-DPS) for reference pixel information processing and its utilization in reconfigurable hardware on-board a spaceflight instrument or post-processing on the ground. The methodology examines signal processing for a 2-D domain, in which high-variance components of the thermal noise are carried by both active and reference pixels, similar to that in processing of low-voltage differential signals and subtraction of a single analog reference pixel from all active pixels on the sensor. Heritage methods using the aforementioned statistical parameters in the

Plasmonic computing of spatial differentiation

Science.gov (United States)

Zhu, Tengfeng; Zhou, Yihan; Lou, Yijie; Ye, Hui; Qiu, Min; Ruan, Zhichao; Fan, Shanhui

2017-05-01

Optical analog computing offers high-throughput low-power-consumption operation for specialized computational tasks. Traditionally, optical analog computing in the spatial domain uses a bulky system of lenses and filters. Recent developments in metamaterials enable the miniaturization of such computing elements down to a subwavelength scale. However, the required metamaterial consists of a complex array of meta-atoms, and direct demonstration of image processing is challenging. Here, we show that the interference effects associated with surface plasmon excitations at a single metal-dielectric interface can perform spatial differentiation. And we experimentally demonstrate edge detection of an image without any Fourier lens. This work points to a simple yet powerful mechanism for optical analog computing at the nanoscale.

Plasmonic computing of spatial differentiation.

Science.gov (United States)

Zhu, Tengfeng; Zhou, Yihan; Lou, Yijie; Ye, Hui; Qiu, Min; Ruan, Zhichao; Fan, Shanhui

2017-05-19

Optical analog computing offers high-throughput low-power-consumption operation for specialized computational tasks. Traditionally, optical analog computing in the spatial domain uses a bulky system of lenses and filters. Recent developments in metamaterials enable the miniaturization of such computing elements down to a subwavelength scale. However, the required metamaterial consists of a complex array of meta-atoms, and direct demonstration of image processing is challenging. Here, we show that the interference effects associated with surface plasmon excitations at a single metal-dielectric interface can perform spatial differentiation. And we experimentally demonstrate edge detection of an image without any Fourier lens. This work points to a simple yet powerful mechanism for optical analog computing at the nanoscale.

A Performance Tuning Methodology with Compiler Support

Directory of Open Access Journals (Sweden)

Oscar Hernandez

2008-01-01

Full Text Available We have developed an environment, based upon robust, existing, open source software, for tuning applications written using MPI, OpenMP or both. The goal of this effort, which integrates the OpenUH compiler and several popular performance tools, is to increase user productivity by providing an automated, scalable performance measurement and optimization system. In this paper we describe our environment, show how these complementary tools can work together, and illustrate the synergies possible by exploiting their individual strengths and combined interactions. We also present a methodology for performance tuning that is enabled by this environment. One of the benefits of using compiler technology in this context is that it can direct the performance measurements to capture events at different levels of granularity and help assess their importance, which we have shown to significantly reduce the measurement overheads. The compiler can also help when attempting to understand the performance results: it can supply information on how a code was translated and whether optimizations were applied. Our methodology combines two performance views of the application to find bottlenecks. The first is a high level view that focuses on OpenMP/MPI performance problems such as synchronization cost and load imbalances; the second is a low level view that focuses on hardware counter analysis with derived metrics that assess the efficiency of the code. Our experiments have shown that our approach can significantly reduce overheads for both profiling and tracing to acceptable levels and limit the number of times the application needs to be run with selected hardware counters. In this paper, we demonstrate the workings of this methodology by illustrating its use with selected NAS Parallel Benchmarks and a cloud resolving code.

Controllability of partial differential equations governed by multiplicative controls

CERN Document Server

Khapalov, Alexander Y

2010-01-01

The goal of this monograph is to address the issue of the global controllability of partial differential equations in the context of multiplicative (or bilinear) controls, which enter the model equations as coefficients. The mathematical models we examine include the linear and nonlinear parabolic and hyperbolic PDE's, the Schrödinger equation, and coupled hybrid nonlinear distributed parameter systems modeling the swimming phenomenon. The book offers a new, high-quality and intrinsically nonlinear methodology to approach the aforementioned highly nonlinear controllability problems.

Application of different methodologies in the preparation of organic matrices for determination of trace elements by differential pulse anodic stripping voltammetry; Aplicacao de diferentes metodologias na preparacao de matrizes organicas para a determinacao voltametrica de elementos traco

Energy Technology Data Exchange (ETDEWEB)

Sisti, Cristina

2001-07-01

The determination of trace elements in food samples is of great importance for the human health, considering the factors of essentiality and toxicity. On the other hand, the chemical analysis is largely affected for the steps of sample preparation; laboratory contamination of the sample and the reagents or still volatilization and losses of the elements. If these parameters are not controlled the achieved precision and accuracy could be low. In this work, the content of zinc, cadmium, lead and copper was determined in adults diet samples collected by duplicate portion technique and bovine liver, applying the differential pulse anodic stripping voltametry - (DP-ASV) technique. In the digestion of the matrices in acid medium, conventional methodologies were used, conductive heating in open recipients and equipment with microwaves source in open and closed vessels. The best procedure was the sample digestion by microwaves, in closed vessels and the other treatments made in controlled atmosphere with hood laminar-airflow class 100. The established methodology was validated with the use of a certified sample as reference (NIST - bovine liver 1577b). (author)

Fluorometric assay for phenotypic differentiation of drug-resistant HIV mutants

OpenAIRE

Zhu, Qinchang; Yu, Zhiqiang; Kabashima, Tsutomu; Yin, Sheng; Dragusha, Shpend; El-Mahdy, Ahmed F. M.; Ejupi, Valon; Shibata, Takayuki; Kai, Masaaki

2015-01-01

Convenient drug-resistance testing of viral mutants is indispensable to effective treatment of viral infection. We developed a novel fluorometric assay for phenotypic differentiation of drug-resistant mutants of human immunodeficiency virus-I protease (HIV-PR) which uses enzymatic and peptide-specific fluorescence (FL) reactions and high-performance liquid chromatography (HPLC) of three HIV-PR substrates. This assay protocol enables use of non-purified enzyme sources and multiple substrates f...

Current status and future prospects for enabling chemistry technology in the drug discovery process [version 1; referees: 2 approved

Directory of Open Access Journals (Sweden)

Stevan W. Djuric

2016-09-01

Full Text Available This review covers recent advances in the implementation of enabling chemistry technologies into the drug discovery process. Areas covered include parallel synthesis chemistry, high-throughput experimentation, automated synthesis and purification methods, flow chemistry methodology including photochemistry, electrochemistry, and the handling of “dangerous” reagents. Also featured are advances in the “computer-assisted drug design” area and the expanding application of novel mass spectrometry-based techniques to a wide range of drug discovery activities.

Methodological possibilities for using the electron and ion energy balance in thermospheric complex measurements

International Nuclear Information System (INIS)

Serafimov, K.B.; Serafimova, M.K.

1991-01-01

Combination of ground based measurements for determination of basic thermospheric characteristics is proposed . An expression for the energy transport between components of space plasma is also derived and discussed within the framework of the presented methodology which could be devided into the folowing major sections: 1) application of ionosonde, absorption measurements, TEC-measurements using Faradey radiation or the differential Doppler effect; 2) ground-based airglow measurements; 3) airglow and palsma satelite measurements. 9 refs

Enabling Semantic Queries Against the Spatial Database

Directory of Open Access Journals (Sweden)

PENG, X.

2012-02-01

Full Text Available The spatial database based upon the object-relational database management system (ORDBMS has the merits of a clear data model, good operability and high query efficiency. That is why it has been widely used in spatial data organization and management. However, it cannot express the semantic relationships among geospatial objects, making the query results difficult to meet the user's requirement well. Therefore, this paper represents an attempt to combine the Semantic Web technology with the spatial database so as to make up for the traditional database's disadvantages. In this way, on the one hand, users can take advantages of ORDBMS to store and manage spatial data; on the other hand, if the spatial database is released in the form of Semantic Web, the users could describe a query more concisely with the cognitive pattern which is similar to that of daily life. As a consequence, this methodology enables the benefits of both Semantic Web and the object-relational database (ORDB available. The paper discusses systematically the semantic enriched spatial database's architecture, key technologies and implementation. Subsequently, we demonstrate the function of spatial semantic queries via a practical prototype system. The query results indicate that the method used in this study is feasible.

Using Differential Item Functioning Procedures to Explore Sources of Item Difficulty and Group Performance Characteristics.

Science.gov (United States)

Scheuneman, Janice Dowd; Gerritz, Kalle

1990-01-01

Differential item functioning (DIF) methodology for revealing sources of item difficulty and performance characteristics of different groups was explored. A total of 150 Scholastic Aptitude Test items and 132 Graduate Record Examination general test items were analyzed. DIF was evaluated for males and females and Blacks and Whites. (SLD)

Prognostics and health management design for rotary machinery systems—Reviews, methodology and applications

Science.gov (United States)

Lee, Jay; Wu, Fangji; Zhao, Wenyu; Ghaffari, Masoud; Liao, Linxia; Siegel, David

2014-01-01

Much research has been conducted in prognostics and health management (PHM), an emerging field in mechanical engineering that is gaining interest from both academia and industry. Most of these efforts have been in the area of machinery PHM, resulting in the development of many algorithms for this particular application. The majority of these algorithms concentrate on applications involving common rotary machinery components, such as bearings and gears. Knowledge of this prior work is a necessity for any future research efforts to be conducted; however, there has not been a comprehensive overview that details previous and on-going efforts in PHM. In addition, a systematic method for developing and deploying a PHM system has yet to be established. Such a method would enable rapid customization and integration of PHM systems for diverse applications. To address these gaps, this paper provides a comprehensive review of the PHM field, followed by an introduction of a systematic PHM design methodology, 5S methodology, for converting data to prognostics information. This methodology includes procedures for identifying critical components, as well as tools for selecting the most appropriate algorithms for specific applications. Visualization tools are presented for displaying prognostics information in an appropriate fashion for quick and accurate decision making. Industrial case studies are included in this paper to show how this methodology can help in the design of an effective PHM system.

Building a RAPPOR with the Unknown: Privacy-Preserving Learning of Associations and Data Dictionaries

Directory of Open Access Journals (Sweden)

Fanti Giulia

2016-07-01

Full Text Available Techniques based on randomized response enable the collection of potentially sensitive data from clients in a privacy-preserving manner with strong local differential privacy guarantees. A recent such technology, RAPPOR [12], enables estimation of the marginal frequencies of a set of strings via privacy-preserving crowdsourcing. However, this original estimation process relies on a known dictionary of possible strings; in practice, this dictionary can be extremely large and/or unknown. In this paper, we propose a novel decoding algorithm for the RAPPOR mechanism that enables the estimation of “unknown unknowns,” i.e., strings we do not know we should be estimating. To enable learning without explicit dictionary knowledge, we develop methodology for estimating the joint distribution of multiple variables collected with RAPPOR. Our contributions are not RAPPOR-specific, and can be generalized to other local differential privacy mechanisms for learning distributions of string-valued random variables.

Potential differentiation of islet-like cells from pregnant cow-derived placental stem cells.

Science.gov (United States)

Peng, Shao-Yu; Chou, Chien-Wen; Kuo, Yu-Hsuan; Shen, Perng-Chih; Shaw, S W Steven

2017-06-01

Type 1 diabetes is an autoimmune disease that destroys islet cells and results in insufficient insulin secretion by pancreatic β-cells. Islet transplantation from donors is an approach used for treating patients with diabetes; however, this therapy is difficult to implement because of the lack of donors. Nevertheless, several stem cells have the potential to differentiate from islet-like cells and enable insulin secretion for treating diabetes in animal models. For example, placenta is considered a waste material and can be harvested noninvasively during delivery without ethical or moral concerns. To date, the differentiation of islet-like cells from cow-derived placental stem cells (CPSCs) has yet to be demonstrated. The investigation of potential differentiation of islet-like cells from CPSCs was conducted by supplementation with nicotinamide, exendin-4, glucose, and poly-d-lysine and was detected through reverse transcription polymerase chain reaction, dithizone staining, and immunocytochemical methods. Our results indicated that CPSCs are established and express mesenchymal stem cell surface antigen markers, such as CD73, CD166, β-integrin, and Oct-4, but not hematopoietic stem cell surface antigen markers, such as CD45. After induction, the CPSCs successfully differentiated into islet-like cells. The CPSC-derived islet-like cells expressed islet cell development-related genes, such as insulin, glucagon, pax-4, Nkx6.1, pax-6, and Fox. Moreover, CPSC-derived islet-like cells can be stained with zinc ions, which are widely distributed in the islet cells and enable insulin secretion. Altogether, islet-like cells have the potential to be differentiated from CPSCs without gene manipulation, and can be used in diabetic animal models in the future for preclinical and drug testing trial investigations. Copyright © 2017. Published by Elsevier B.V.

Differentiation of Pseudomonas syringae Pathovars Originating from Stone Fruits

Directory of Open Access Journals (Sweden)

Katarina Gašić

2012-01-01

Full Text Available Due to an overlapping host range, similar symptomatology and many common characteristics,Pseudomonas syringae pathovars originating from stone fruits can easily be misidentified.In order to select tests for rapid and efficient differentiation of P. s. pvs. syringae,morsprunorum and persicae, we studied the suitability and differentiating potential ofsome standard bacteriological and molecular methods. Differentiation of the strains wasperformed using LOPAT, GATTa and ice nucleation tests, nutrient sucrose broth growthand utilization of various carbon sources. PCR method enabled the detection of toxin-producinggenes: syrB and syrD in P. s. pv. syringae, and cfl gene in P. s. pv. morsprunorum race1. Syringomycin production by pv. syringae was confirmed in bioassay using Geotrichumcandidum, Saccharomyces cerevisiae and Rhodotorula pilimanae as indicator organisms.Pathogenicity test on lemon and immature nectarine fruits, as well as on string bean pods,showed different intensity of reaction of the inoculated material which could separate pv.syringae from the other two pathovars. PCR-based repetitive sequences, Rep-PCR withREP, ERIC and BOX primers revealed different genetic profiles within P. syringae pathovars.

Optical Imaging for Stem Cell Differentiation to Neuronal Lineage

International Nuclear Information System (INIS)

Hwang, Do Won; Lee, Dong Soo

2012-01-01

In regenerative medicine, the prospect of stem cell therapy hold great promise for the recovery of injured tissues and effective treatment of intractable diseases. Tracking stem cell fate provides critical information to understand and evaluate the success of stem cell therapy. The recent emergence of in vivo noninvasive molecular imaging has enabled assessment of the behavior of grafted stem cells in living subjects. In this review, we provide an overview of current optical imaging strategies based on cell or tissue specific reporter gene expression and of in vivo methods to monitor stem cell differentiation into neuronal lineages. These methods use optical reporters either regulated by neuron-specific promoters or containing neuron-specific microRNA binding sites. Both systems revealed dramatic changes in optical reporter imaging signals in cells differentiating a yeast GAL4 amplification system or an engineering-enhanced luciferase reported gene. Furthermore, we propose an advanced imaging system to monitor neuronal differentiation during neurogenesis that uses in vivo multiplexed imaging techniques capable of detecting several targets simultaneously

The need for standardization of methodology and components in commercial radioimmunoassay kits

International Nuclear Information System (INIS)

Wood, W.G.; Marschner, I.; Scriba, P.C.

1978-01-01

The problems arising from the increasing use of commercial kits in radioimmunoassay (RIA) and related fields are discussed. These problems differ according to the substance under test. The quality of individual reagents is often good, but the methodology is often not optimal and may contain short-cuts which, although commercially attractive, can lead to erroneous values and poor sensitivity and precision. Minor modifications in the methodology often lead to big improvements in sensitivity and precision. This has been demonstrated in three digoxin kits employing antibody-coated tube techniques and in four kits for thyrotropin (TSH) using different techniques. It has also been noted that with many quality-control sera imported from the USA no values are ascribed to European kits for the components listed, thus reducing these sera to the function of precision control. The study underlines the need to standardize kit components and assay methods to enable the results obtained by different laboratories with different kits to be compared. (author)

Partial wave analysis for folded differential cross sections

Science.gov (United States)

Machacek, J. R.; McEachran, R. P.

2018-03-01

The value of modified effective range theory (MERT) and the connection between differential cross sections and phase shifts in low-energy electron scattering has long been recognized. Recent experimental techniques involving magnetically confined beams have introduced the concept of folded differential cross sections (FDCS) where the forward (θ ≤ π/2) and backward scattered (θ ≥ π/2) projectiles are unresolved, that is the value measured at the angle θ is the sum of the signal for particles scattered into the angles θ and π - θ. We have developed an alternative approach to MERT in order to analyse low-energy folded differential cross sections for positrons and electrons. This results in a simplified expression for the FDCS when it is expressed in terms of partial waves and thereby enables one to extract the first few phase shifts from a fit to an experimental FDCS at low energies. Thus, this method predicts forward and backward angle scattering (0 to π) using only experimental FDCS data and can be used to determine the total elastic cross section solely from experimental results at low-energy, which are limited in angular range.

Graphical Methodology of Global Pollution Index for the Environmental Impact Assessment Using Two Environmental Components

OpenAIRE

Corneliu Cojocaru; Diana Mariana Cocârţă; Irina Aura Istrate; Igor Creţescu

2017-01-01

One of the applied methods for environmental impact assessment is the index of global pollution (IGP) proposed by Rojanschi in 1991. This methodology enables the global estimation for the ecosystem state affected more or less by human activities. Unfortunately, Rojanschi’s method has a limitation; it can be applied only if at least three environmental components are considered. Frequently, many environmental impact assessment applications rely on analysis of only two environmental components....

AEGIS methodology and a perspective from AEGIS methodology demonstrations

International Nuclear Information System (INIS)

Dove, F.H.

1981-03-01

Objectives of AEGIS (Assessment of Effectiveness of Geologic Isolation Systems) are to develop the capabilities needed to assess the post-closure safety of waste isolation in geologic formation; demonstrate these capabilities on reference sites; apply the assessment methodology to assist the NWTS program in site selection, waste package and repository design; and perform repository site analyses for the licensing needs of NWTS. This paper summarizes the AEGIS methodology, the experience gained from methodology demonstrations, and provides an overview in the following areas: estimation of the response of a repository to perturbing geologic and hydrologic events; estimation of the transport of radionuclides from a repository to man; and assessment of uncertainties

Going beyond a First Reader: A Machine Learning Methodology for Optimizing Cost and Performance in Breast Ultrasound Diagnosis.

Science.gov (United States)

Venkatesh, Santosh S; Levenback, Benjamin J; Sultan, Laith R; Bouzghar, Ghizlane; Sehgal, Chandra M

2015-12-01

The goal of this study was to devise a machine learning methodology as a viable low-cost alternative to a second reader to help augment physicians' interpretations of breast ultrasound images in differentiating benign and malignant masses. Two independent feature sets consisting of visual features based on a radiologist's interpretation of images and computer-extracted features when used as first and second readers and combined by adaptive boosting (AdaBoost) and a pruning classifier resulted in a very high level of diagnostic performance (area under the receiver operating characteristic curve = 0.98) at a cost of pruning a fraction (20%) of the cases for further evaluation by independent methods. AdaBoost also improved the diagnostic performance of the individual human observers and increased the agreement between their analyses. Pairing AdaBoost with selective pruning is a principled methodology for achieving high diagnostic performance without the added cost of an additional reader for differentiating solid breast masses by ultrasound. Copyright © 2015 World Federation for Ultrasound in Medicine & Biology. Published by Elsevier Inc. All rights reserved.

Three-dimensional printed knotted reactors enabling highly sensitive differentiation of silver nanoparticles and ions in aqueous environmental samples

Energy Technology Data Exchange (ETDEWEB)

Su, Cheng-Kuan, E-mail: chengkuan@ntou.edu.tw [Department of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, 20224, Taiwan, ROC (China); Hsieh, Meng-Hsuan [Department of Biomedical Engineering and Environmental Sciences, National Tsing-Hua University, Hsinchu, 30013, Taiwan, ROC (China); Sun, Yuh-Chang, E-mail: ycsun@mx.nthu.edu.tw [Department of Biomedical Engineering and Environmental Sciences, National Tsing-Hua University, Hsinchu, 30013, Taiwan, ROC (China)

2016-03-31

Whether silver nanoparticles (AgNPs) persist or release silver ions (Ag{sup +}) when discharged into a natural environment has remained an unresolved issue. In this study, we employed a low-cost stereolithographic three-dimensional printing (3DP) technology to fabricate the angle-defined knotted reactors (KRs) to construct a simple differentiation scheme for quantitative assessment of Ag{sup +} ions and AgNPs in municipal wastewater samples. We chose xanthan/phosphate-buffered saline as a dispersion medium for in situ stabilization of the two silver species, while also facilitating their extraction from complicated wastewater matrices. After method optimization, we measured extraction efficiencies of 54.5 and 32.3% for retaining Ag{sup +} ions and AgNPs, respectively, in the printed KR (768-turn), with detection limits (DLs) of 0.86 and 0.52 ng L{sup −1} when determining Ag{sup +} ions and AgNPs, respectively (sample run at pH 11 without a rinse solution), and 0.86 ng L{sup −1} when determining Ag{sup +} ions alone (sample run at pH 12 with a 1.5-mL rinse solution). The proposed scheme is tolerant of the wastewater matrix and provides more reliable differentiation between Ag{sup +}/AgNPs than does a conventional filtration method. The concept and applicability of adopting 3DP technology to renew traditional KR devices were evidently proven by means of these significantly improved analytical performance. Our analytical data suggested that the concentrations of Ag{sup +} ions and AgNPs in the tested industrial wastewater sample were both higher than those in domestic wastewater, implying that industrial activity might be a main source of environmental silver species, rather than domestic discharge from AgNP-containing products. - Highlights: • 3D printed knotted reactors are utilized to differentiate AgNPs and Ag{sup +} ions. • Xanthan/phosphate-buffered saline is used for stabilizing the two silver species. • Extraction efficiency up to 54.5% is

Differential Expression of Adhesion-Related Proteins and MAPK Pathways Lead to Suitable Osteoblast Differentiation of Human Mesenchymal Stem Cells Subpopulations.

Science.gov (United States)

Leyva-Leyva, Margarita; López-Díaz, Annia; Barrera, Lourdes; Camacho-Morales, Alberto; Hernandez-Aguilar, Felipe; Carrillo-Casas, Erika M; Arriaga-Pizano, Lourdes; Calderón-Pérez, Jaime; García-Álvarez, Jorge; Orozco-Hoyuela, Gabriel; Piña-Barba, Cristina; Rojas-Martínez, Augusto; Romero-Díaz, Víktor; Lara-Arias, Jorge; Rivera-Bolaños, Nancy; López-Camarillo, César; Moncada-Saucedo, Nidia; Galván-De los Santos, Alejandra; Meza-Urzúa, Fátima; Villarreal-Gómez, Luis; Fuentes-Mera, Lizeth

2015-11-01

Cellular adhesion enables communication between cells and their environment. Adhesion can be achieved throughout focal adhesions and its components influence osteoblast differentiation of human mesenchymal stem cells (hMSCs). Because cell adhesion and osteoblast differentiation are closely related, this article aimed to analyze the expression profiles of adhesion-related proteins during osteoblastic differentiation of two hMSCs subpopulations (CD105(+) and CD105(-)) and propose a strategy for assembling bone grafts based on its adhesion ability. In vitro experiments of osteogenic differentiation in CD105(-) cells showed superior adhesion efficiency and 2-fold increase of α-actinin expression compared with CD105(+) cells at the maturation stage. Interestingly, levels of activated β1-integrin increased in CD105(-) cells during the process. Additionally, the CD105(-) subpopulation showed 3-fold increase of phosphorylated FAK(Y397) compared to CD105(+) cells. Results also indicate that ERK1/2 was activated during CD105(-) bone differentiation and participation of mitogen-activated protein kinase (MAPK)-p38 in CD105(+) differentiation through a focal adhesion kinase (FAK)-independent pathway. In vivo trial demonstrated that grafts containing CD105(-) showed osteocytes embedded in a mineralized matrix, promoted adequate graft integration, increased host vascular infiltration, and efficient intramembranous repairing. In contrast, grafts containing CD105(+) showed deficient endochondral ossification and fibrocartilaginous tissue. Based on the expression of α-actinin, FAKy,(397) and ERK1/2 activation, we define maturation stage as critical for bone graft assembling. By in vitro assays, CD105(-) subpopulation showed superior adhesion efficiency compared to CD105(+) cells. Considering in vitro and in vivo assays, this study suggests that integration of a scaffold with CD105(-) subpopulation at the maturation stage represents an attractive strategy for clinical use in

New understanding of rhizosphere processes enabled by advances in molecular and spatially resolved techniques

Energy Technology Data Exchange (ETDEWEB)

Hess, Nancy J.; Paša-Tolić, Ljiljana; Bailey, Vanessa L.; Dohnalkova, Alice C.

2017-06-01

Understanding the role played by microorganisms within soil systems is challenged by the unique intersection of physics, chemistry, mineralogy and biology in fostering habitat for soil microbial communities. To address these challenges will require observations across multiple spatial and temporal scales to capture the dynamics and emergent behavior from complex and interdependent processes. The heterogeneity and complexity of the rhizosphere require advanced techniques that press the simultaneous frontiers of spatial resolution, analyte sensitivity and specificity, reproducibility, large dynamic range, and high throughput. Fortunately many exciting technical advancements are now available to inform and guide the development of new hypotheses. The aim of this Special issue is to provide a holistic view of the rhizosphere in the perspective of modern molecular biology methodologies that enabled a highly-focused, detailed view on the processes in the rhizosphere, including numerous, strong and complex interactions between plant roots, soil constituents and microorganisms. We discuss the current rhizosphere research challenges and knowledge gaps, as well as perspectives and approaches using newly available state-of-the-art toolboxes. These new approaches and methodologies allow the study of rhizosphere processes and properties, and rhizosphere as a central component of ecosystems and biogeochemical cycles.

Early development of executive functions: a differential study

Directory of Open Access Journals (Sweden)

Sylvia Sastre-Riba

2015-05-01

Full Text Available The ontogeny of executive functions is essential in explaining differential and normative developmental trends. Executive functions must be studied from an early age given their consequential effects on mental flexibility, monitoring information, planning, and cognitive control. We propose a differential study in alternative developmental courses through observing typical babies, Down syndrome babies, and babies with risk-factors at birth (due to low weight or to congenital hypothyroidism. Applymg Systematic Observational Methodology, spontaneous babies' activity was registered. The results indicated that: a Typical babies showed better shifting and action flexibility in order to obtain a goal, thus better results; b Among the higher risk-babies, the lower efficacy in executive functioning was observed in underweight babies. Those with hypothyroidism were more in line with the typical babies; c Underweight babies showed a good level of combining actions but they obtained inferior results; d Down syndrome babies displayed more executive functioning difficulty, lower flexibility, high perseveration and less error detection.

Nordic Housing Enabler

DEFF Research Database (Denmark)

Helle, Tina; Brandt, Åse

Development and reliability testing of the Nordic Housing Enabler – an instrument for accessibility assessment of the physical housing. Tina Helle & Åse Brandt University of Lund, Health Sciences, Faculty of Medicine (SE) and University College Northern Jutland, Occupational Therapy department (DK......). Danish Centre for Assistive Technology. Abstract. For decades, accessibility to the physical housing environment for people with functional limitations has been of interest politically, professionally and for the users. Guidelines and norms on accessible housing design have gradually been developed......, however, the built environment shows serious deficits when it comes to accessibility. This study addresses development of a content valid cross-Nordic version of the Housing Enabler and investigation of inter-rater reliability, when used in occupational therapy practice. The instrument was translated from...

Differential Intracochlear Sound Pressure Measurements in Normal Human Temporal Bones

Science.gov (United States)

Nakajima, Hideko Heidi; Dong, Wei; Olson, Elizabeth S.; Merchant, Saumil N.; Ravicz, Michael E.; Rosowski, John J.

2009-02-01

We present the first simultaneous sound pressure measurements in scala vestibuli and scala tympani of the cochlea in human cadaveric temporal bones. Micro-scale fiberoptic pressure sensors enabled the study of differential sound pressure at the cochlear base. This differential pressure is the input to the cochlear partition, driving cochlear waves and auditory transduction. Results showed that: pressure of scala vestibuli was much greater than scala tympani except at low and high frequencies where scala tympani pressure affects the input to the cochlea; the differential pressure proved to be an excellent measure of normal ossicular transduction of sound (shown to decrease 30-50 dB with ossicular disarticulation, whereas the individual scala pressures were significantly affected by non-ossicular conduction of sound at high frequencies); the middle-ear gain and differential pressure were generally bandpass in frequency dependence; and the middle-ear delay in the human was over twice that of the gerbil. Concurrent stapes velocity measurements allowed determination of the differential impedance across the partition and round-window impedance. The differential impedance was generally resistive, while the round-window impedance was consistent with a compliance in conjunction with distributed inertia and damping. Our techniques can be used to study inner-ear conductive pathologies (e.g., semicircular dehiscence), as well as non-ossicular cochlear stimulation (e.g., round-window stimulation) - situations that cannot be completely quantified by measurements of stapes velocity or scala-vestibuli pressure by themselves.

Clinical trial methodology

National Research Council Canada - National Science Library

Peace, Karl E; Chen, Ding-Geng

2011-01-01

... in the pharmaceutical industry, Clinical trial methodology emphasizes the importance of statistical thinking in clinical research and presents the methodology as a key component of clinical research...

Applying of component system development in object methodology, case study

Directory of Open Access Journals (Sweden)

Milan Mišovič

2013-01-01

Full Text Available To create computarization target software as a component system has been a very strong requirement for the last 20 years of software developing. Finally, the architectural components are self-contained units, presenting not only partial and overall system behavior, but also cooperating with each other on the basis of their interfaces. Among others, components have allowed flexible modification of processes the behavior of which is the foundation of components behavior without changing the life of the component system. On the other hand, the component system makes it possible, at design time, to create numerous new connections between components and thus creating modified system behaviors. This all enables the company management to perform, at design time, required behavioral changes of processes in accordance with the requirements of changing production and market.The development of software which is generally referred to as SDP (Software Development Process contains two directions. The first one, called CBD (Component–Based Development, is dedicated to the development of component–based systems CBS (Component–based System, the second target is the development of software under the influence of SOA (Service–Oriented Architecture. Both directions are equipped with their different development methodologies. The subject of this paper is only the first direction and application of development of component–based systems in its object–oriented methodologies. The requirement of today is to carry out the development of component-based systems in the framework of developed object–oriented methodologies precisely in the way of a dominant style. In some of the known methodologies, however, this development is not completely transparent and is not even recognized as dominant. In some cases, it is corrected by the special meta–integration models of component system development into an object methodology.This paper presents a case study

A combination of differential method and perturbation theory for the calculation of sensitivity coefficients

International Nuclear Information System (INIS)

Santos, Adimir dos; Borges, A.A.

2000-01-01

A new method for the calculation of sensitivity coefficients is developed. The new method is a combination of two methodologies used for calculating these coefficients, which are the differential and the generalized perturbation theory methods. The proposed method utilizes as integral parameter the average flux in an arbitrary region of the system. Thus, the sensitivity coefficient contains only the component corresponding to the neutron flux. To obtain the new sensitivity coefficient, the derivates of the integral parameter, φ(ξ), with respect to σ are calculated using the perturbation method and the functional derivates of this generic integral parameter with respect to σ and φ are calculated using the differential method. The new method merges the advantages of the differential and generalized perturbation theory methods and eliminates their disadvantages. (author)

Methodological and hermeneutic reduction - a study of Finnish multiple-birth families.

Science.gov (United States)

Heinonen, Kristiina

2015-07-01

To describe reduction as a method in methodological and hermeneutic reduction and the hermeneutic circle using van Manen's principles, with the empirical example of the lifeworlds of multiple-birth families in Finland. Reduction involves several levels that can be distinguished for their methodological usefulness. Researchers can use reduction in different ways and dimensions for their methodological needs. Open interviews with public health nurses, family care workers and parents of twins. The systematic literature and knowledge review shows there were no articles on multiple-birth families that used van Manen's method. This paper presents reduction as a method that uses the hermeneutic circle. The lifeworlds of multiple-birth families consist of three core themes: 'A state of constant vigilance'; 'Ensuring that they can continue to cope'; and 'Opportunities to share with other people'. Reduction allows us to perform deep phenomenological-hermeneutic research and understand people's lifeworlds. It helps to keep research stages separate but also enables a consolidated view. Social care and healthcare professionals have to hear parents' voices better to comprehensively understand their situation; they also need further tools and training to be able to empower parents of twins. The many variations in adapting reduction mean its use can be very complex and confusing. This paper adds to the discussion of phenomenology, hermeneutic study and reduction.

PCR methodology as a valuable tool for identification of endodontic pathogens.

Science.gov (United States)

Siqueira, José F; Rôças, Isabela N

2003-07-01

This paper reviews the principles of polymerase chain reaction (PCR) methodology, its application in identification of endodontic pathogens and the perspectives regarding the knowledge to be reached with the use of this highly sensitive, specific and accurate methodology as a microbial identification test. Studies published in the medical, dental and biological literature. Evaluation of published epidemiological studies examining the endodontic microbiota through PCR methodology. PCR technology has enabled the detection of bacterial species that are difficult or even impossible to culture as well as cultivable bacterial strains showing a phenotypically divergent or convergent behaviour. Moreover, PCR is more rapid, much more sensitive, and more accurate when compared with culture. Its use in endodontics to investigate the microbiota associated with infected root canals has expanded the knowledge on the bacteria involved in the pathogenesis of periradicular diseases. For instance, Tannerella forsythensis (formerly Bacteroides forsythus), Treponema denticola, other Treponema species, Dialister pneumosintes, and Prevotella tannerae were detected in infected root canals for the first time and in high prevalence when using PCR analysis. The diversity of endodontic microbiota has been demonstrated by studies using PCR amplification, cloning and sequencing of the PCR products. Moreover, other fastidious bacterial species, such as Porphyromonas endodontalis, Porphyromonas gingivalis and some Eubacterium spp., have been reported in endodontic infections at a higher prevalence than those reported by culture procedures.

H + H2 on LEPS and Porter-Karplus surfaces;Quasiclassical differential cross sections for reactive scattering

International Nuclear Information System (INIS)

Jorgensen, A.D.; Gislason, E.A.; Hillenbrand, E.A.

1981-01-01

The reactive differential cross section is determined by the use of a fourier sine series for the H + H 2 reaction on the Porter Karplus and LEPS surfaces. The A + BC program was used to run quasiclassical trajectories. Saddle-point properties are compared, including those for SLTH surfaces. The use of the Fourier sine series enables one to obtain very accurate differential cross sections, allowing precise comparison of the reaction dynamics on different potential energy surfaces and at different energies

Enabling private and public sector organizations as agents of homeland security

Science.gov (United States)

Glassco, David H. J.; Glassco, Jordan C.

2006-05-01

Homeland security and defense applications seek to reduce the risk of undesirable eventualities across physical space in real-time. With that functional requirement in mind, our work focused on the development of IP based agent telecommunication solutions for heterogeneous sensor / robotic intelligent "Things" that could be deployed across the internet. This paper explains how multi-organization information and device sharing alliances may be formed to enable organizations to act as agents of homeland security (in addition to other uses). Topics include: (i) using location-aware, agent based, real-time information sharing systems to integrate business systems, mobile devices, sensor and actuator based devices and embedded devices used in physical infrastructure assets, equipment and other man-made "Things"; (ii) organization-centric real-time information sharing spaces using on-demand XML schema formatted networks; (iii) object-oriented XML serialization as a methodology for heterogeneous device glue code; (iv) how complex requirements for inter / intra organization information and device ownership and sharing, security and access control, mobility and remote communication service, tailored solution life cycle management, service QoS, service and geographic scalability and the projection of remote physical presence (through sensing and robotics) and remote informational presence (knowledge of what is going elsewhere) can be more easily supported through feature inheritance with a rapid agent system development methodology; (v) how remote object identification and tracking can be supported across large areas; (vi) how agent synergy may be leveraged with analytics to complement heterogeneous device networks.

Capturing complexity in work disability research: application of system dynamics modeling methodology.

Science.gov (United States)

Jetha, Arif; Pransky, Glenn; Hettinger, Lawrence J

2016-01-01

Work disability (WD) is characterized by variable and occasionally undesirable outcomes. The underlying determinants of WD outcomes include patterns of dynamic relationships among health, personal, organizational and regulatory factors that have been challenging to characterize, and inadequately represented by contemporary WD models. System dynamics modeling (SDM) methodology applies a sociotechnical systems thinking lens to view WD systems as comprising a range of influential factors linked by feedback relationships. SDM can potentially overcome limitations in contemporary WD models by uncovering causal feedback relationships, and conceptualizing dynamic system behaviors. It employs a collaborative and stakeholder-based model building methodology to create a visual depiction of the system as a whole. SDM can also enable researchers to run dynamic simulations to provide evidence of anticipated or unanticipated outcomes that could result from policy and programmatic intervention. SDM may advance rehabilitation research by providing greater insights into the structure and dynamics of WD systems while helping to understand inherent complexity. Challenges related to data availability, determining validity, and the extensive time and technical skill requirements for model building may limit SDM's use in the field and should be considered. Contemporary work disability (WD) models provide limited insight into complexity associated with WD processes. System dynamics modeling (SDM) has the potential to capture complexity through a stakeholder-based approach that generates a simulation model consisting of multiple feedback loops. SDM may enable WD researchers and practitioners to understand the structure and behavior of the WD system as a whole, and inform development of improved strategies to manage straightforward and complex WD cases.

In silico ordinary differential equation/partial differential equation hemodialysis model estimates methadone removal during dialysis

Science.gov (United States)

Linares, Oscar A; Schiesser, William E; Fudin, Jeffrey; Pham, Thien C; Bettinger, Jeffrey J; Mathew, Roy O; Daly, Annemarie L

2015-01-01

Background There is a need to have a model to study methadone’s losses during hemodialysis to provide informed methadone dose recommendations for the practitioner. Aim To build a one-dimensional (1-D), hollow-fiber geometry, ordinary differential equation (ODE) and partial differential equation (PDE) countercurrent hemodialyzer model (ODE/PDE model). Methodology We conducted a cross-sectional study in silico that evaluated eleven hemodialysis patients. Patients received a ceiling dose of methadone hydrochloride 30 mg/day. Outcome measures included: the total amount of methadone removed during dialysis; methadone’s overall intradialytic mass transfer rate coefficient, km; and, methadone’s removal rate, jME. Each metric was measured at dialysate flow rates of 250 mL/min and 800 mL/min. Results The ODE/PDE model revealed a significant increase in the change of methadone’s mass transfer with increased dialysate flow rate, %Δkm=18.56, P=0.02, N=11. The total amount of methadone mass transferred across the dialyzer membrane with high dialysate flow rate significantly increased (0.042±0.016 versus 0.052±0.019 mg/kg, P=0.02, N=11). This was accompanied by a small significant increase in methadone’s mass transfer rate (0.113±0.002 versus 0.014±0.002 mg/kg/h, P=0.02, N=11). The ODE/PDE model accurately predicted methadone’s removal during dialysis. The absolute value of the prediction errors for methadone’s extraction and throughput were less than 2%. Conclusion ODE/PDE modeling of methadone’s hemodialysis is a new approach to study methadone’s removal, in particular, and opioid removal, in general, in patients with end-stage renal disease on hemodialysis. ODE/PDE modeling accurately quantified the fundamental phenomena of methadone’s mass transfer during hemodialysis. This methodology may lead to development of optimally designed intradialytic opioid treatment protocols, and allow dynamic monitoring of outflow plasma opioid concentrations for model

Pilot project as enabler?

DEFF Research Database (Denmark)

Neisig, Margit; Glimø, Helle; Holm, Catrine Granzow

This article deals with a systemic perspective on transition. The field of study addressed is a pilot project as enabler of transition in a highly complex polycentric context. From a Luhmannian systemic approach, a framework is created to understand and address barriers of change occurred using...... pilot projects as enabler of transition. Aspects of how to create trust and deal with distrust during a transition are addressed. The transition in focus is the concept of New Public Management and how it is applied in the management of the Employment Service in Denmark. The transition regards...

Symbiotic Cell Differentiation and Cooperative Growth in Multicellular Aggregates.

Directory of Open Access Journals (Sweden)

Jumpei F Yamagishi

2016-10-01

Full Text Available As cells grow and divide under a given environment, they become crowded and resources are limited, as seen in bacterial biofilms and multicellular aggregates. These cells often show strong interactions through exchanging chemicals, as evident in quorum sensing, to achieve mutualism and division of labor. Here, to achieve stable division of labor, three characteristics are required. First, isogenous cells differentiate into several types. Second, this aggregate of distinct cell types shows better growth than that of isolated cells without interaction and differentiation, by achieving division of labor. Third, this cell aggregate is robust with respect to the number distribution of differentiated cell types. Indeed, theoretical studies have thus far considered how such cooperation is achieved when the ability of cell differentiation is presumed. Here, we address how cells acquire the ability of cell differentiation and division of labor simultaneously, which is also connected with the robustness of a cell society. For this purpose, we developed a dynamical-systems model of cells consisting of chemical components with intracellular catalytic reaction dynamics. The reactions convert external nutrients into internal components for cellular growth, and the divided cells interact through chemical diffusion. We found that cells sharing an identical catalytic network spontaneously differentiate via induction from cell-cell interactions, and then achieve division of labor, enabling a higher growth rate than that in the unicellular case. This symbiotic differentiation emerged for a class of reaction networks under the condition of nutrient limitation and strong cell-cell interactions. Then, robustness in the cell type distribution was achieved, while instability of collective growth could emerge even among the cooperative cells when the internal reserves of products were dominant. The present mechanism is simple and general as a natural consequence of

Introducing soft systems methodology plus (SSM+): why we need it and what it can contribute.

Science.gov (United States)

Braithwaite, Jeffrey; Hindle, Don; Iedema, Rick; Westbrook, Johanna I

2002-01-01

There are many complicated and seemingly intractable problems in the health care sector. Past ways to address them have involved political responses, economic restructuring, biomedical and scientific studies, and managerialist or business-oriented tools. Few methods have enabled us to develop a systematic response to problems. Our version of soft systems methodology, SSM+, seems to improve problem solving processes by providing an iterative, staged framework that emphasises collaborative learning and systems redesign involving both technical and cultural fixes.

Development of a Pattern Recognition Methodology for Determining Operationally Optimal Heat Balance Instrumentation Calibration Schedules

Energy Technology Data Exchange (ETDEWEB)

Kurt Beran; John Christenson; Dragos Nica; Kenny Gross

2002-12-15

The goal of the project is to enable plant operators to detect with high sensitivity and reliability the onset of decalibration drifts in all of the instrumentation used as input to the reactor heat balance calculations. To achieve this objective, the collaborators developed and implemented at DBNPS an extension of the Multivariate State Estimation Technique (MSET) pattern recognition methodology pioneered by ANAL. The extension was implemented during the second phase of the project and fully achieved the project goal.

Reliability Centered Maintenance - Methodologies

Science.gov (United States)

Kammerer, Catherine C.

2009-01-01

Journal article about Reliability Centered Maintenance (RCM) methodologies used by United Space Alliance, LLC (USA) in support of the Space Shuttle Program at Kennedy Space Center. The USA Reliability Centered Maintenance program differs from traditional RCM programs because various methodologies are utilized to take advantage of their respective strengths for each application. Based on operational experience, USA has customized the traditional RCM methodology into a streamlined lean logic path and has implemented the use of statistical tools to drive the process. USA RCM has integrated many of the L6S tools into both RCM methodologies. The tools utilized in the Measure, Analyze, and Improve phases of a Lean Six Sigma project lend themselves to application in the RCM process. All USA RCM methodologies meet the requirements defined in SAE JA 1011, Evaluation Criteria for Reliability-Centered Maintenance (RCM) Processes. The proposed article explores these methodologies.

A novel algorithm for discrimination between inrush current and internal faults in power transformer differential protection based on discrete wavelet transform

Energy Technology Data Exchange (ETDEWEB)

Eldin, A.A. Hossam; Refaey, M.A. [Electrical Engineering Department, Alexandria University, Alexandria (Egypt)

2011-01-15

This paper proposes a novel methodology for transformer differential protection, based on wave shape recognition of the discriminating criterion extracted of the instantaneous differential currents. Discrete wavelet transform has been applied to the differential currents due to internal fault and inrush currents. The diagnosis criterion is based on median absolute deviation (MAD) of wavelet coefficients over a specified frequency band. The proposed algorithm is examined using various simulated inrush and internal fault current cases on a power transformer that has been modeled using electromagnetic transients program EMTDC software. Results of evaluation study show that, proposed wavelet based differential protection scheme can discriminate internal faults from inrush currents. (author)

Differential diagnosis and staging of urological tumors by magnetic resonance imaging compared with computed tomography

International Nuclear Information System (INIS)

Nishimura, Kazuo; Okada, Yusaku; Takeuchi, Hideo; Miyakawa, Mieko; Okada, Kenichiro; Yoshida, Osamu; Nishimura, Kazumasa

1987-01-01

Magnetic resonance imaging (MRI) was performed on 49 urological tumors (11 renal cell carcinomas, 3 renal pelvic cancers, 2 renal angiomyolipomas, 1 renal leiomyosarcoma, 1 large renal cvst, 4 adrenal tumors, 11 bladder cancers, 2 bone metastasis from bladder cancer, 10 prostatic cancers, 1 prostatic sarcoma, 1 urethral cancer, 1 penile cancer and 1 perivesical granuloma) since October 1985 to September 1986. MRI was performed using a Signa (G.E.) with a 1.5 T superconductive magnet and 3 images, including T1 weighted image, T2 weighted image, and proton density image, were obtained. In conclusion MRI is a noninvasive examination and gives more information than computed tomography despite its high cost. In renal cell carcinoma, the chemical shift in MRI and clear visualization of tumor thrombus enable accurate staging. Differential diagnosis from other renal mass lesions may be possible by the T2 weighted image. In adrenal disease, most of the adrenal masses can be differentiated, but in some cases it is impossible. In bladder cancer, wall invasion of tumor may be evaluated in T2 weighted image, and MRI is suitable for staging of locally advanced tumor. In prostatic cancer, visualization of periprostatic plexus and differentiation between internal and external gland may enable local staging and identification of low stage tumors. (author)

Development of Testing Methodologies for the Mechanical Properties of MEMS

Science.gov (United States)

Ekwaro-Osire, Stephen

2003-01-01

This effort is to investigate and design testing strategies to determine the mechanical properties of MicroElectroMechanical Systems (MEMS) as well as investigate the development of a MEMS Probabilistic Design Methodology (PDM). One item of potential interest is the design of a test for the Weibull size effect in pressure membranes. The Weibull size effect is a consequence of a stochastic strength response predicted from the Weibull distribution. Confirming that MEMS strength is controlled by the Weibull distribution will enable the development of a probabilistic design methodology for MEMS - similar to the GRC developed CARES/Life program for bulk ceramics. However, the primary area of investigation will most likely be analysis and modeling of material interfaces for strength as well as developing a strategy to handle stress singularities at sharp corners, filets, and material interfaces. This will be a continuation of the previous years work. The ultimate objective of this effort is to further develop and verify the ability of the Ceramics Analysis and Reliability Evaluation of Structures Life (CARES/Life) code to predict the time-dependent reliability of MEMS structures subjected to multiple transient loads.

Design verification methodology for a solenoid valve for industrial applications

International Nuclear Information System (INIS)

Park, Chang Dae; Lim, Byung Ju; Chun, Kyung Yul

2015-01-01

Solenoid operated valves (SOV) are widely used in many applications due to their fast dynamic responses, cost effectiveness, and less contamination sensitive characteristics. In this paper, we tried to provide a convenient method of design verification of SOV to design engineers who depend on their experiences and experiment during design and development process of SOV. First, we summarize a detailed procedure for designing SOVs for industrial applications. All of the design constraints are defined in the first step of the design, and then the detail design procedure is presented based on design experiences as well as various physical and electromagnetic relationships. Secondly, we have suggested a verification method of this design using theoretical relationships, which enables optimal design of SOV from a point of view of safety factor of design attraction force. Lastly, experimental performance tests using several prototypes manufactured based on this design method show that the suggested design verification methodology is appropriate for designing new models of solenoids. We believe that this verification process is novel logic and useful to save time and expenses during development of SOV because verification tests with manufactured specimen may be substituted partly by this verification methodology.

Discounting in agro-industrial complex. A methodological proposal for risk premium

Energy Technology Data Exchange (ETDEWEB)

Marques-Perez, I.; Guaita-Pradas, I.; Pérez-Salas, J.L.

2017-09-01

The estimation of the discount rate is decisive for a reliable economic valuation. The discount rate has to be adjusted for the risks related to the company, the sector which the company has its market, and the risks related to the investment project. We present a proposal to incorporate the risk premium to the discount rate. The novelty of the methodology is that difference risk groups according to activity as a factor to adjust the cost of capital to companies. The study applies the methodology to the Agro-Industrial Complex (AIC) in Spain. The AIC is formed by industries that add value to farming production. This sector’s economic success demands financial management techniques that assess risk. The conventional method responds neither to the heterogeneity of the economic activities that make up the AIC, nor to differentiating risk by groups. The proposed methodology distinguishes activity groups in accordance with the NACE (National Code of Economic Activities) and uses net profitability variability to distinguish the risk in each group. Our results demonstrate the various levels of risk per group. The results show that among all the groups that form the AIC there are wide differences between levels of risk; thus, the risk neutral groups present risk levels on the order of 150 times lower than the groups extreme risk levels.

Discounting in agro-industrial complex. A methodological proposal for risk premium

Directory of Open Access Journals (Sweden)

Inmaculada Marques-Perez

2017-04-01

Full Text Available The estimation of the discount rate is decisive for a reliable economic valuation. The discount rate has to be adjusted for the risks related to the company, the sector which the company has its market, and the risks related to the investment project. We present a proposal to incorporate the risk premium to the discount rate. The novelty of the methodology is that difference risk groups according to activity as a factor to adjust the cost of capital to companies. The study applies the methodology to the Agro-Industrial Complex (AIC in Spain. The AIC is formed by industries that add value to farming production. This sector’s economic success demands financial management techniques that assess risk. The conventional method responds neither to the heterogeneity of the economic activities that make up the AIC, nor to differentiating risk by groups. The proposed methodology distinguishes activity groups in accordance with the NACE (National Code of Economic Activities and uses net profitability variability to distinguish the risk in each group. Our results demonstrate the various levels of risk per group. The results show that among all the groups that form the AIC there are wide differences between levels of risk; thus, the risk neutral groups present risk levels on the order of 150 times lower than the groups extreme risk levels.

Discounting in agro-industrial complex. A methodological proposal for risk premium

International Nuclear Information System (INIS)

Marques-Perez, I.; Guaita-Pradas, I.; Pérez-Salas, J.L.

2017-01-01

The estimation of the discount rate is decisive for a reliable economic valuation. The discount rate has to be adjusted for the risks related to the company, the sector which the company has its market, and the risks related to the investment project. We present a proposal to incorporate the risk premium to the discount rate. The novelty of the methodology is that difference risk groups according to activity as a factor to adjust the cost of capital to companies. The study applies the methodology to the Agro-Industrial Complex (AIC) in Spain. The AIC is formed by industries that add value to farming production. This sector’s economic success demands financial management techniques that assess risk. The conventional method responds neither to the heterogeneity of the economic activities that make up the AIC, nor to differentiating risk by groups. The proposed methodology distinguishes activity groups in accordance with the NACE (National Code of Economic Activities) and uses net profitability variability to distinguish the risk in each group. Our results demonstrate the various levels of risk per group. The results show that among all the groups that form the AIC there are wide differences between levels of risk; thus, the risk neutral groups present risk levels on the order of 150 times lower than the groups extreme risk levels.

Simulated Microgravity Modulates Differentiation Processes of Embryonic Stem Cells

Directory of Open Access Journals (Sweden)

Vaibhav Shinde

2016-04-01

Full Text Available Background/Aims: Embryonic developmental studies under microgravity conditions in space are very limited. To study the effects of altered gravity on the embryonic development processes we established an in vitro methodology allowing differentiation of mouse embryonic stem cells (mESCs under simulated microgravity within a fast-rotating clinostat (clinorotation and capture of microarray-based gene signatures. Methods: The differentiating mESCs were cultured in a 2D pipette clinostat. The microarray and bioinformatics tools were used to capture genes that are deregulated by simulated microgravity and their impact on developmental biological processes. Results: The data analysis demonstrated that differentiation of mESCs in pipettes for 3 days resultet to early germ layer differentiation and then to the different somatic cell types after further 7 days of differentiation in the Petri dishes. Clinorotation influences differentiation as well as non-differentiation related biological processes like cytoskeleton related 19 genes were modulated. Notably, simulated microgravity deregulated genes Cyr61, Thbs1, Parva, Dhrs3, Jun, Tpm1, Fzd2 and Dll1 are involved in heart morphogenesis as an acute response on day 3. If the stem cells were further cultivated under normal gravity conditions (1 g after clinorotation, the expression of cardiomyocytes specific genes such as Tnnt2, Rbp4, Tnni1, Csrp3, Nppb and Mybpc3 on day 10 was inhibited. This correlated well with a decreasing beating activity of the 10-days old embryoid bodies (EBs. Finally, we captured Gadd45g, Jun, Thbs1, Cyr61and Dll1 genes whose expressions were modulated by simulated microgravity and by real microgravity in various reported studies. Simulated microgravity also deregulated genes belonging to the MAP kinase and focal dhesion signal transduction pathways. Conclusion: One of the most prominent biological processes affected by simulated microgravity was the process of cardiomyogenesis. The

SystemVerilog assertions and functional coverage guide to language, methodology and applications

CERN Document Server

Mehta, Ashok B

2013-01-01

This book provides a hands-on, application-oriented guide to the language and methodology of both SystemVerilog Assertions and SytemVerilog Functional Coverage.  Readers will benefit from the step-by-step approach to functional hardware verification, which will enable them to uncover hidden and hard to find bugs, point directly to the source of the bug, provide for a clean and easy way to model complex timing checks and objectively answer the question 'have we functionally verified everything'.  Written by a professional end-user of both SystemVerilog Assertions and SystemVerilog Functional Co

KIC 9451096: Magnetic Activity, Flares and Differential Rotation

Science.gov (United States)

Özdarcan, O.; Yoldaş, E.; Dal, H. A.

2018-04-01

We present a spectroscopic and photometric analysis of KIC 9451096. The combined spectroscopic and photometric modelling shows that the system is a detached eclipsing binary in a circular orbit and composed of F5V + K2V components. Subtracting the best-fitting light curve model from the whole long cadence data reveals additional low (mmag) amplitude light variations in time and occasional flares, suggesting a low, but still remarkable level of magnetic spot activity on the K2V component. Analyzing the rotational modulation of the light curve residuals enables us to estimate the differential rotation coefficient of the K2V component as k = 0.069 ± 0.008, which is 3 times weaker compared with the solar value of k = 0.19, assuming a solar type differential rotation. We find the stellar flare activity frequency for the K2V component as 0.000368411 h-1 indicating a low magnetic activity level.

Promoting Continuous Quality Improvement in the Alabama Child Health Improvement Alliance Through Q-Sort Methodology and Learning Collaboratives.

Science.gov (United States)

Fifolt, Matthew; Preskitt, Julie; Rucks, Andrew; Corvey, Kathryn; Benton, Elizabeth Cason

Q-sort methodology is an underutilized tool for differentiating among multiple priority measures. The authors describe steps to identify, delimit, and sort potential health measures and use selected priority measures to establish an overall agenda for continuous quality improvement (CQI) activities within learning collaboratives. Through an iterative process, the authors vetted a list of potential child and adolescent health measures. Multiple stakeholders, including payers, direct care providers, and organizational representatives sorted and prioritized measures, using Q-methodology. Q-methodology provided the Alabama Child Health Improvement Alliance (ACHIA) an objective and rigorous approach to system improvement. Selected priority measures were used to design learning collaboratives. An open dialogue among stakeholders about state health priorities spurred greater organizational buy-in for ACHIA and increased its credibility as a statewide provider of learning collaboratives. The integrated processes of Q-sort methodology, learning collaboratives, and CQI offer a practical yet innovative way to identify and prioritize state measures for child and adolescent health and establish a learning agenda for targeted quality improvement activities.

Organisational Learning and the Organisational Life Cycle: The Differential Aspects of an Integrated Relationship in SMEs

Science.gov (United States)

Tam, Steven; Gray, David E.

2016-01-01

Purpose: The purpose of this study is to relate the practice of organisational learning in small- and medium-sized enterprises (SMEs) to the organisational life cycle (OLC), contextualising the differential aspects of an integrated relationship between them. Design/methodology/approach: It is a mixed-method study with two consecutive phases. In…

Measuring the impact of methodological research: a framework and methods to identify evidence of impact.

Science.gov (United States)

Brueton, Valerie C; Vale, Claire L; Choodari-Oskooei, Babak; Jinks, Rachel; Tierney, Jayne F

2014-11-27

gathered in this study to adapt an existing framework for impact of clinical research for use in methodological research. Gathering evidence on research impact of methodological research from a variety of sources has enabled us to obtain multiple indicators and thus to demonstrate broad impacts of methodological research. The adapted framework developed can be applied to future methodological research and thus provides a tool for methodologists to better assess and report research impacts.

Perspective for special Gurdon issue for differentiation: can cell fusion inform nuclear reprogramming?

Science.gov (United States)

Burns, David; Blau, Helen M

2014-07-01

Nuclear reprogramming was first shown to be possible by Sir John Gurdon over a half century ago. The process has been revolutionized by the production of induced pluripotent cells by overexpression of the four transcription factors discovered by Shinya Yamanaka, which now enables mammalian applications. Yet, reprogramming by a few transcription factors remains incomplete and inefficient, whether to pluripotent or differentiated cells. We propose that a better understanding of mechanistic insights based on developmental principles gained from heterokaryon studies may inform the process of directing cell fate, fundamentally and clinically. Copyright © 2014 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.

Functions of Heterogeneous Nuclear Ribonucleoproteins in Stem Cell Potency and Differentiation

Directory of Open Access Journals (Sweden)

Qishan Chen

2013-01-01

Full Text Available Stem cells possess huge importance in developmental biology, disease modelling, cell replacement therapy, and tissue engineering in regenerative medicine because they have the remarkable potential for self-renewal and to differentiate into almost all the cell types in the human body. Elucidation of molecular mechanisms regulating stem cell potency and differentiation is essential and critical for extensive application. Heterogeneous nuclear ribonucleoproteins (hnRNPs are modular proteins consisting of RNA-binding motifs and auxiliary domains characterized by extensive and divergent functions in nucleic acid metabolism. Multiple roles of hnRNPs in transcriptional and posttranscriptional regulation enable them to be effective gene expression regulators. More recent findings show that hnRNP proteins are crucial factors implicated in maintenance of stem cell self-renewal and pluripotency and cell differentiation. The hnRNPs interact with certain sequences in target gene promoter regions to initiate transcription. In addition, they recognize 3′UTR or 5′UTR of specific gene mRNA forming mRNP complex to regulate mRNA stability and translation. Both of these regulatory pathways lead to modulation of gene expression that is associated with stem cell proliferation, cell cycle control, pluripotency, and committed differentiation.

AIREK-MOD, Time Dependent Reactor Kinetics with Feedback Differential Equation

International Nuclear Information System (INIS)

Tamagnini, C.

1984-01-01

1 - Nature of physical problem solved: Solves the reactor kinetic equations with respect to time. A standard form for the reactivity behaviour has been introduced in which the reactivity is given by the sum of a polynomial, sine, cosine and exponential expansion. Tabular form is also included. The presence of feedback differential equations in which the dependence on variables different from the considered one is considered enables many heat-exchange problems to be dealt with. 2 - Method of solution: The method employed for the solution of the differential equations is the one developed by E.R. Cohen (Geneva Conference, 1958). 3 - Restrictions on the complexity of the problem: The maximum number of differential equations that can be solved simultaneously is 50. Within this limitation there may be n delayed neutron groups (n less than or equal to 25), on m other linear feedback equations (n+m less than or equal to 49). CDC 1604 version was offered by EIR (Institut Federal de Recherches en matiere de reacteurs, Switzerland)

A combination between the differential and the perturbation theory methods for calculating sensitivity coefficients

International Nuclear Information System (INIS)

Borges, Antonio Andrade

1998-01-01

A new method for the calculation of sensitivity coefficients is developed. The new method is a combination of two methodologies used for calculating theses coefficients, which are the differential and the generalized perturbation theory methods. The method utilizes as integral parameter the average flux in an arbitrary region of the system. Thus, the sensitivity coefficient contains only the component corresponding to the neutron flux. To obtain the new sensitivity coefficient, the derivatives of the integral parameter, Φ, with respect to σ are calculated using the perturbation method and the functional derivatives of this generic integral parameter with respect to σ and Φ are calculated using the differential method. (author)

A spatially-evaluated methodology for assessing risk to a population from contaminated land

International Nuclear Information System (INIS)

Gay, J. Rebecca; Korre, Anna

2006-01-01

A methodology is proposed which combines quantitative probabilistic human health risk assessment and spatial statistical methods (geostatistics) to produce an assessment of risks to human health from exposure to contaminated land, in a manner which preserves the spatial distribution of risks and provides a measure of uncertainty in the assessment. Maps of soil contaminant levels, which incorporate uncertainty, are produced from sparse sample data using sequential indicator simulation. A real, age-stratified population is mapped across the contaminated area, and intake of soil contaminants by individuals is calculated probabilistically using an adaptation of the Contaminated Land Exposure Assessment (CLEA) model. An abundance of information is contained in results which can be interrogated at the population and individual level, and mapped to provide a powerful visual tool for risk managers, enabling efficient targeting of risk reduction measures to different locations. - A methodology for calculating and mapping risks to a population from intake of soil contaminants

Barriers and enablers to implementing scalp cooling in Australia: a qualitative study of health professionals' attitudes to and experience with scalp cooling.

Science.gov (United States)

Shaw, Joanne M; O'Brien, Jane; Chua, Susan; De Boer, Richard; Dear, Rachel; Murray, Nicholas; Boyle, Fran

2018-01-01

Chemotherapy-induced alopecia is a common and distressing adverse event for patients. Scalp cooling to reduce this alopecia has been available in Europe for more than a decade, but only recently introduced in Australia. The aim of this study was to qualitatively explore health professionals' perceptions of the barriers and enablers to the implementation of scalp cooling in Australian cancer centres. Using a qualitative methodology, telephone interviews were conducted with 21 health professionals working in a tumour stream where chemotherapy-induced alopecia is an adverse event of treatment. Participants were recruited from five centres in Australia where scalp cooling is currently available and one centre without access to the technology. Four interrelated themes were identified: (1) health professional attitudes, (2) concerns for patient equity, (3) logistical considerations and (4) organisational support. This qualitative study provides the first methodological exploration of Australian health professionals' perceptions of barriers and enablers to scalp cooling uptake. The results highlighted health professional support drives the introduction of scalp cooling. Integration of the technology requires adjustments to nursing practice to manage the increased time, workload and change in patient flow. Strategies to manage the change in practice and organisational support for change in work flow are essential for successful implementation into routine care.

A business intelligence approach using web search tools and online data reduction techniques to examine the value of product-enabled services

DEFF Research Database (Denmark)

Tanev, Stoyan; Liotta, Giacomo; Kleismantas, Andrius

2015-01-01

in Canada and Europe. It adopts an innovative methodology based on online textual data that could be implemented in advanced business intelligence tools aiming at the facilitation of innovation, marketing and business decision making. Combinations of keywords referring to different aspects of service value......-service innovation as a competitive advantage on the marketplace. On the other hand, the focus of EU firms on innovative hybrid offerings is not explicitly related to business differentiation and competitiveness....

A demand-centered, hybrid life-cycle methodology for city-scale greenhouse gas inventories.

Science.gov (United States)

Ramaswami, Anu; Hillman, Tim; Janson, Bruce; Reiner, Mark; Thomas, Gregg

2008-09-01

Greenhouse gas (GHG) accounting for individual cities is confounded by spatial scale and boundary effects that impact the allocation of regional material and energy flows. This paper develops a demand-centered, hybrid life-cycle-based methodology for conducting city-scale GHG inventories that incorporates (1) spatial allocation of surface and airline travel across colocated cities in larger metropolitan regions, and, (2) life-cycle assessment (LCA) to quantify the embodied energy of key urban materials--food, water, fuel, and concrete. The hybrid methodology enables cities to separately report the GHG impact associated with direct end-use of energy by cities (consistent with EPA and IPCC methods), as well as the impact of extra-boundary activities such as air travel and production of key urban materials (consistent with Scope 3 protocols recommended by the World Resources Institute). Application of this hybrid methodology to Denver, Colorado, yielded a more holistic GHG inventory that approaches a GHG footprint computation, with consistency of inclusions across spatial scale as well as convergence of city-scale per capita GHG emissions (approximately 25 mt CO2e/person/year) with state and national data. The method is shown to have significant policy impacts, and also demonstrates the utility of benchmarks in understanding energy use in various city sectors.

AN INDUCTIVE, INTERACTIVE AND ADAPTIVE HYBRID PROBLEM-BASED LEARNING METHODOLOGY: APPLICATION TO STATISTICS

Directory of Open Access Journals (Sweden)

ADA ZHENG

2011-10-01

Full Text Available We have developed an innovative hybrid problem-based learning (PBL methodology. The methodology has the following distinctive features: i Each complex question was decomposed into a set of coherent finer subquestions by following the carefully designed criteria to maintain a delicate balance between guiding the students and inspiring them to think independently. This learning methodology enabled the students to solve the complex questions progressively in an inductive context. ii Facilitated by the utilization of our web-based learning systems, the teacher was able to interact with the students intensively and could allocate more teaching time to provide tailor-made feedback for individual student. The students were actively engaged in the learning activities, stimulated by the intensive interaction. iii The answers submitted by the students could be automatically consolidated in the report of the Moodle system in real-time. The teacher could adjust the teaching schedule and focus of the class to adapt to the learning progress of the students by analysing the automatically generated report and log files of the web-based learning system. As a result, the attendance rate of the students increased from about 50% to more than 90%, and the students’ learning motivation have been significantly enhanced.

Synthetic niches for differentiation of human embryonic stem cells bypassing embryoid body formation.

Science.gov (United States)

Liu, Yarong; Fox, Victoria; Lei, Yuning; Hu, Biliang; Joo, Kye-Il; Wang, Pin

2014-07-01

The unique self-renewal and pluripotency features of human embryonic stem cells (hESCs) offer the potential for unlimited development of novel cell therapies. Currently, hESCs are cultured and differentiated using methods, such as monolayer culture and embryoid body (EB) formation. As such, achieving efficient differentiation into higher order structures remains a challenge, as well as maintaining cell viability during differentiation into homogeneous cell populations. Here, we describe the application of highly porous polymer scaffolds as synthetic stem cell niches. Bypassing the EB formation step, these scaffolds are capable of three-dimensional culture of undifferentiated hESCs and subsequent directed differentiation into three primary germ layers. H9 hESCs were successfully maintained and proliferated in biodegradable polymer scaffolds based on poly (lactic-co-glycolic acid) (PLGA). The results showed that cells within PLGA scaffolds retained characteristics of undifferentiated pluripotent stem cells. Moreover, the scaffolds allowed differentiation towards the lineage of interest by the addition of growth factors to the culture system. The in vivo transplantation study revealed that the scaffolds could provide a microenvironment that enabled hESCs to interact with their surroundings, thereby promoting cell differentiation. Therefore, this approach, which provides a unique culture/differentiation system for hESCs, will find its utility in various stem cell-based tissue-engineering applications. © 2013 Wiley Periodicals, Inc.

Macro-level enabling conditions for the formation of social business enterprises in the Philippines

Directory of Open Access Journals (Sweden)

Aaron Laylo

2018-05-01

Full Text Available Purpose - A conducive and enabling environment is imperative for the formation of sustainable social business enterprises (SBEs. This paper aims to identify the macro-level enabling conditions necessary for SBE formation and to analyze them in the context of the Philippines, an emerging economy that is yet to be transformed into an inclusive one. Design/methodology/approach - Major developments on micro-, small- and medium-sized enterprises, specifically on social enterprises, were revisited and analyzed. The author also looked into how they are sustained, supported and nurtured in the Philippines’ overall economic landscape. Extensive data were collected from relevant agencies in public and private sectors, after which they were analyzed parallel to existing academic literature, i.e. theories, models and concepts, on social entrepreneurship and development nexus. Findings - It has been found that the four macro-level enabling conditions, namely, governance, socially inclusive economic approach, financial services and entrepreneurial culture, presumed to be vital for SBE formation, contribute to the latter at various levels, but surely complement each other in the process. Research limitations/implications - The significance of exploring the context in which social enterprises are formed and flourish lies in the sheer importance of understanding the sustained prevalence of SBEs in many economies – both in developed and developing ones. Originality/value - By having a more structured knowledge of the components surrounding SBE formation, the community may be able to also simultaneously explore why and how social entrepreneurs form profit-earning business entities that are primarily driven by social advocacies and goals.

Situating methodology within qualitative research.

Science.gov (United States)

Kramer-Kile, Marnie L

2012-01-01

Qualitative nurse researchers are required to make deliberate and sometimes complex methodological decisions about their work. Methodology in qualitative research is a comprehensive approach in which theory (ideas) and method (doing) are brought into close alignment. It can be difficult, at times, to understand the concept of methodology. The purpose of this research column is to: (1) define qualitative methodology; (2) illuminate the relationship between epistemology, ontology and methodology; (3) explicate the connection between theory and method in qualitative research design; and 4) highlight relevant examples of methodological decisions made within cardiovascular nursing research. Although there is no "one set way" to do qualitative research, all qualitative researchers should account for the choices they make throughout the research process and articulate their methodological decision-making along the way.

Service models and realization of differentiated services networks

Science.gov (United States)

Elizondo, Antonio J.; Garcia Osma, Maria L.; Einsiedler, Hans J.; Roth, Rudolf; Smirnov, Michael I.; Bartoli, Maurizio; Castelli, Paolo; Varga, Balazs; Krampell, Magnus

2001-07-01

Internet Service Providers need to offer Quality of Service (QoS) to fulfil the requirements of applications of their customers. Moreover, in a competitive market environment costs must be low. The selected service model must be effective and low in complexity, but it should still provide high quality and service differentiation, that the current Internet is not yet capable to support. The Differentiated Services (DiffServ) Architecture has been proposed for enabling a range of different Classes of Service (CoS). In the EURESCOM project P1006 several European service providers co-operated to examine various aspects involved in the introduction of service differentiation using the DiffServ approach. The project explored a set of service models for Expedited Forwarding (EF) and Assured Forwarding (AF) and identified requirements for network nodes. Besides, we addressed also measurement issues, charging and accounting issues. Special attention has been devoted to requirements of elastic traffic that adapts its sending rate to congestion state and available bandwidth. QoS mechanisms must prove Transmission Control Protocol (TCP) friendliness. TCP performance degrades under multiple losses. Since RED based queue management may still cause multiple discards, a modified marking scheme called Capped Leaky Bucket is proposed to improve the performance of elastic applications.

Estimating significances of differences between slopes: A new methodology and software

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Vasco M. N. C. S. Vieira

2013-09-01

Full Text Available Determining the significance of slope differences is a common requirement in studies of self-thinning, ontogeny and sexual dimorphism, among others. This has long been carried out testing for the overlap of the bootstrapped 95% confidence intervals of the slopes. However, the numerical random re-sampling with repetition favours the occurrence of re-combinations yielding largely diverging slopes, widening the confidence intervals and thus increasing the chances of overlooking significant differences. To overcome this problem a permutation test simulating the null hypothesis of no differences between slopes is proposed. This new methodology, when applied both to artificial and factual data, showed an enhanced ability to differentiate slopes.

High-Resolution Amplified Fragment Length Polymorphism Typing of Lactococcus lactis Strains Enables Identification of Genetic Markers for Subspecies-Related Phenotypes▿

Science.gov (United States)

Kütahya, Oylum Erkus; Starrenburg, Marjo J. C.; Rademaker, Jan L. W.; Klaassen, Corné H. W.; van Hylckama Vlieg, Johan E. T.; Smid, Eddy J.; Kleerebezem, Michiel

2011-01-01

A high-resolution amplified fragment length polymorphism (AFLP) methodology was developed to achieve the delineation of closely related Lactococcus lactis strains. The differentiation depth of 24 enzyme-primer-nucleotide combinations was experimentally evaluated to maximize the number of polymorphisms. The resolution depth was confirmed by performing diversity analysis on 82 L. lactis strains, including both closely and distantly related strains with dairy and nondairy origins. Strains clustered into two main genomic lineages of L. lactis subsp. lactis and L. lactis subsp. cremoris type-strain-like genotypes and a third novel genomic lineage rooted from the L. lactis subsp. lactis genomic lineage. Cluster differentiation was highly correlated with small-subunit rRNA homology and multilocus sequence analysis (MLSA) studies. Additionally, the selected enzyme-primer combination generated L. lactis subsp. cremoris phenotype-specific fragments irrespective of the genotype. These phenotype-specific markers allowed the differentiation of L. lactis subsp. lactis phenotype from L. lactis subsp. cremoris phenotype strains within the same L. lactis subsp. cremoris type-strain-like genomic lineage, illustrating the potential of AFLP for the generation of phenotype-linked genetic markers. PMID:21666014

Modulated Temperature Differential Scanning Calorimetry Theoretical and Practical Applications in Polymer Characterisation

CERN Document Server

Reading, Mike

2006-01-01

MTDSC provides a step-change increase in the power of calorimetry to characterize virtually all polymer systems including curing systems, blends and semicrystalline polymers. It enables hidden transitions to be revealed, miscibility to be accurately assessed, and phases and interfaces in complex blends to be quantified. It also enables crystallinity in complex systems to be measured and provides new insights into melting behaviour. All of this is achieved by a simple modification of conventional DSC. In 1992 a new calorimetric technique was introduced that superimposed a small modulation on top of the conventional linear temperature program typically used in differential scanning calorimetry. This was combined with a method of data analysis that enabled the sample’s response to the linear component of the temperature program to be separated from its response to the periodic component. In this way, for the first time, a signal equivalent to that of conventional DSC was obtained simultaneously with a measure ...

Synchrotron-based nu-XRF mapping and mu-FTIR microscopy enable to look into the fate and effects of tattoo pigments in human skin

OpenAIRE

Schreiver , Ines; Hesse , Bernhard; Seim , Christian; Castillo-Michel , Hiram; Villanova , Julie; Laux , Peter; Dreiack , Nadine; Penning , Randolf; Tucoulou , Remi; Cotte , Marine; Luch , Andreas

2017-01-01

International audience; The increasing prevalence of tattoos provoked safety concerns with respect to particle distribution and effects inside the human body. We used skin and lymphatic tissues from human corpses to address local biokinetics by means of synchrotron X-ray fluorescence (XRF) techniques at both the micro (mu) and nano (nu) scale. Additional advanced mass spectrometry-based methodology enabled to demonstrate simultaneous transport of organic pigments, heavy metals and titanium di...

Methodology of sustainability accounting

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O.H. Sokil

2017-03-01

Full Text Available Modern challenges of the theory and methodology of accounting are realized through the formation and implementation of new concepts, the purpose of which is to meet the needs of users in standard and unique information. The development of a methodology for sustainability accounting is a key aspect of the management of an economic entity. The purpose of the article is to form the methodological bases of accounting for sustainable development and determine its goals, objectives, object, subject, methods, functions and key aspects. The author analyzes the theoretical bases of the definition and considers the components of the traditional accounting methodology. Generalized structural diagram of the methodology for accounting for sustainable development is offered in the article. The complex of methods and principles of sustainable development accounting for systematized and non-standard provisions has been systematized. The new system of theoretical and methodological provisions of accounting for sustainable development is justified in the context of determining its purpose, objective, subject, object, methods, functions and key aspects.

OGC® Sensor Web Enablement Standards

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George Percivall

2006-09-01

Full Text Available This article provides a high-level overview of and architecture for the Open Geospatial Consortium (OGC standards activities that focus on sensors, sensor networks, and a concept called the “Sensor Web”. This OGC work area is known as Sensor Web Enablement (SWE. This article has been condensed from "OGC® Sensor Web Enablement: Overview And High Level Architecture," an OGC White Paper by Mike Botts, PhD, George Percivall, Carl Reed, PhD, and John Davidson which can be downloaded from http://www.opengeospatial.org/pt/15540. Readers interested in greater technical and architecture detail can download and read the OGC SWE Architecture Discussion Paper titled “The OGC Sensor Web Enablement Architecture” (OGC document 06-021r1, http://www.opengeospatial.org/pt/14140.

Comparison Of Irms Delhi Methodology With Who Methodology On Immunization Coverage

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Singh Padam

1996-01-01

Full Text Available Research question: What are the merits of IRMS Model over WHO Model for Coverage Evaluation Survey? Which method is superior and appropriate for coverage evolution survey of immunization in our setting? Objective: To compare IRMS Delhi methodology with WHO methodology on Immunization Coverage. Study Design: Cross-Sectional Setting: Urban and Rural both. Participants: Mothers& Children Sample Size: 300 children between 1-2 years and 300 mothers in rural areas and 75 children and 75 mothers in urban areas. Study Variables: Rural, Urban, Cast-Group, Size of the stratum, Literacy, Sex and Cost effectiveness. Outcome Variables: Coverage level of immunization. Analysis: Routine Statistical Analysis. Results: IRMS developed methodology scores better rating over WHO methodology, especially when coverage evolution is attempted in medium size villages with existence of socio-economic seggregation-which remains the main characteristic of the Indian villages.

Enabling Open Innovation: Lessons from Haier

Institute of Scientific and Technical Information of China (English)

Arie Y.Lewin; Liisa V(a)likangas; Jin Chen

2017-01-01

Open innovation has become a dominant innovation paradigm.However,the actual adoption of open innovation organizational designs and practices remains elusive,and ongoing examples of large companies practicing open innovation in mature industries or beyond R&D activities are rare.Despite the continuing interest in open innovation and the surging research on the topic,not much is documented about how,in particular,large companies interpret and implement open innovation or develop and sustain an innovation-enabling culture.This paper reports on a study of Haier's adoption of six radical innovations as it implements an open innovation organization over a period of seven years.The study is unique in that the cases reveal how open innovation is enabled by the socially enabling mechanisms developed under Chairman Ruimin Zhang's leadership.These varied enabling mechanisms open the organization to serendipity at every level,from the bottom up to suppliers.Most importantly,the mechanisms imprint and sustain an open innovation culture recognized as important-yet often left unarticulated in terms of how it is practiced-in the prior literature.The paper contributes to and highlights the centrality of socially enabling mechanisms underlying an organization's innovation absorptive capacity.

Selection methodology for LWR safety programs and proposals. Volume 2. Methodology application

International Nuclear Information System (INIS)

Ritzman, R.L.; Husseiny, A.A.

1980-08-01

The results of work done to update and apply a methodology for selecting (prioritizing) LWR safety technology R and D programs are described. The methodology is based on multiattribute utility (MAU) theory. Application of the methodology to rank-order a group of specific R and D programs included development of a complete set of attribute utility functions, specification of individual attribute scaling constants, and refinement and use of an interactive computer program (MAUP) to process decision-maker inputs and generate overall (multiattribute) program utility values. The output results from several decision-makers are examined for consistency and conclusions and recommendations regarding general use of the methodology are presented. 3 figures, 18 tables

Rapid Industrial Prototyping and SoC Design of 3G/4G Wireless Systems Using an HLS Methodology

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Andres Takach

2006-07-01

Full Text Available Many very-high-complexity signal processing algorithms are required in future wireless systems, giving tremendous challenges to real-time implementations. In this paper, we present our industrial rapid prototyping experiences on 3G/4G wireless systems using advanced signal processing algorithms in MIMO-CDMA and MIMO-OFDM systems. Core system design issues are studied and advanced receiver algorithms suitable for implementation are proposed for synchronization, MIMO equalization, and detection. We then present VLSI-oriented complexity reduction schemes and demonstrate how to interact these high-complexity algorithms with an HLS-based methodology for extensive design space exploration. This is achieved by abstracting the main effort from hardware iterations to the algorithmic C/C++ fixed-point design. We also analyze the advantages and limitations of the methodology. Our industrial design experience demonstrates that it is possible to enable an extensive architectural analysis in a short-time frame using HLS methodology, which significantly shortens the time to market for wireless systems.

Rapid Industrial Prototyping and SoC Design of 3G/4G Wireless Systems Using an HLS Methodology

Directory of Open Access Journals (Sweden)

Cavallaro JosephR

2006-01-01

Full Text Available Many very-high-complexity signal processing algorithms are required in future wireless systems, giving tremendous challenges to real-time implementations. In this paper, we present our industrial rapid prototyping experiences on 3G/4G wireless systems using advanced signal processing algorithms in MIMO-CDMA and MIMO-OFDM systems. Core system design issues are studied and advanced receiver algorithms suitable for implementation are proposed for synchronization, MIMO equalization, and detection. We then present VLSI-oriented complexity reduction schemes and demonstrate how to interact these high-complexity algorithms with an HLS-based methodology for extensive design space exploration. This is achieved by abstracting the main effort from hardware iterations to the algorithmic C/C++ fixed-point design. We also analyze the advantages and limitations of the methodology. Our industrial design experience demonstrates that it is possible to enable an extensive architectural analysis in a short-time frame using HLS methodology, which significantly shortens the time to market for wireless systems.

Architecture of a service-enabled sensing platform for the environment.

Science.gov (United States)

Kotsev, Alexander; Pantisano, Francesco; Schade, Sven; Jirka, Simon

2015-02-13

Recent technological advancements have led to the production of arrays of miniaturized sensors, often embedded in existing multitasking devices (e.g., smartphones, tablets) and using a wide range of radio standards (e.g., Bluetooth, Wi-Fi, 4G cellular networks). Altogether, these technological evolutions coupled with the diffusion of ubiquitous Internet connectivity provide the base-line technology for the Internet of Things (IoT). The rapid increase of IoT devices is enabling the definition of new paradigms of data collection and introduces the concept of mobile crowd-sensing. In this respect, new sensing methodologies promise to extend the current understanding of the environment and social behaviors by leveraging citizen-contributed data for a wide range of applications. Environmental sensing can however only be successful if all the heterogeneous technologies and infrastructures work smoothly together. As a result, the interconnection and orchestration of devices is one of the central issues of the IoT paradigm. With this in mind, we propose an approach for improving the accessibility of observation data, based on interoperable standards and on-device web services.

Architecture of a Service-Enabled Sensing Platform for the Environment

Directory of Open Access Journals (Sweden)

Alexander Kotsev

2015-02-01

Full Text Available Recent technological advancements have led to the production of arrays of miniaturized sensors, often embedded in existing multitasking devices (e.g., smartphones, tablets and using a wide range of radio standards (e.g., Bluetooth, Wi-Fi, 4G cellular networks. Altogether, these technological evolutions coupled with the diffusion of ubiquitous Internet connectivity provide the base-line technology for the Internet of Things (IoT. The rapid increase of IoT devices is enabling the definition of new paradigms of data collection and introduces the concept of mobile crowd-sensing. In this respect, new sensing methodologies promise to extend the current understanding of the environment and social behaviors by leveraging citizen-contributed data for a wide range of applications. Environmental sensing can however only be successful if all the heterogeneous technologies and infrastructures work smoothly together. As a result, the interconnection and orchestration of devices is one of the central issues of the IoT paradigm. With this in mind, we propose an approach for improving the accessibility of observation data, based on interoperable standards and on-device web services.

Differential evolution to enhance localization of mobile robots

DEFF Research Database (Denmark)

Lisowski, Michal; Fan, Zhun; Ravn, Ole

2011-01-01

. In addition, a novel mechanism for effective robot kidnap detection was proposed. Experiments were performed using computer simulations based on the odometer data and laser range finder measurements collected in advance by a robot in real-life. Experimental results showed that integrating DE enables MCL...... to provide more accurate robot pose estimations in shorter time while using fewer particles.......This paper focuses on the mobile robot localization problems: pose tracking, global localization and robot kidnap. Differential Evolution (DE) applied to extend Monte Carlo Localization (MCL) was investigated to better solve localization problem by increasing localization reliability and speed...

A methodology for selection of wind energy system locations using multicriterial analysis

Energy Technology Data Exchange (ETDEWEB)

Sansevic, M.; Rabadan, Lj. Pilic [Croatia Univ., Faculty of Electrical Engineering, Mechanical Engineering and Naval Architecture, Split (Croatia)

1996-12-31

The effectiveness of a wind turbine generator depends not only on its performance but also on the site`s wind resource. Thus the problem of location selection should be approached systematically, by considering a set of relevant parameters particularly those having a significant economical and ecological impact. This paper presents the methodology used in location selection for the operation of wind energy system. It is based on a multicriterial analysis which enables comparison and ranking of locations according to a set of different parameters. Principal objectives (criteria) in location selection are: energy-economical, technical-technological, physical planning and environment and life protection objectives. For the mathematical modeling of this multicriterial problem the PROMETHEE method is chosen which is developed especially for the solution of rather ``poorly`` structured problems, thus justifying its application in the preliminary stage of site selection for the wind energy systems. The developed methodology is applied in selecting the locations in the island of Rhodes using the available database of the Geographic Information System and the wind potential data obtained by means of the AIOLOS program. (Author)

Kaupapa Maori Methodology: Trusting the Methodology through Thick and Thin

Science.gov (United States)

Hiha, Anne Aroha

2016-01-01

Kaupapa Maori is thoroughly theorised in academia in Aotearoa and those wishing to use it as their research methodology can find support through the writing of a number of Maori academics. What is not so well articulated, is the experiential voice of those who have used Kaupapa Maori as research methodology. My identity as a Maori woman…

PC analysis of stochastic differential equations driven by Wiener noise

KAUST Repository

Le Maitre, Olivier

2015-03-01

A polynomial chaos (PC) analysis with stochastic expansion coefficients is proposed for stochastic differential equations driven by additive or multiplicative Wiener noise. It is shown that for this setting, a Galerkin formalism naturally leads to the definition of a hierarchy of stochastic differential equations governing the evolution of the PC modes. Under the mild assumption that the Wiener and uncertain parameters can be treated as independent random variables, it is also shown that the Galerkin formalism naturally separates parametric uncertainty and stochastic forcing dependences. This enables us to perform an orthogonal decomposition of the process variance, and consequently identify contributions arising from the uncertainty in parameters, the stochastic forcing, and a coupled term. Insight gained from this decomposition is illustrated in light of implementation to simplified linear and non-linear problems; the case of a stochastic bifurcation is also considered.

Barriers and enablers to implementing multiple stroke guideline recommendations: a qualitative study.

Science.gov (United States)

McCluskey, Annie; Vratsistas-Curto, Angela; Schurr, Karl

2013-08-19

Translating evidence into practice is an important final step in the process of evidence-based practice. Medical record audits can be used to examine how well practice compares with published evidence, and identify evidence-practice gaps. After providing audit feedback to professionals, local barriers to practice change can be identified and targetted with focussed behaviour change interventions. This study aimed to identify barriers and enablers to implementing multiple stroke guideline recommendations at one Australian stroke unit. A qualitative methodology was used. A sample of 28 allied health, nursing and medical professionals participated in a group or individual interview. These interviews occurred after staff had received audit feedback and identified areas for practice change. Questions focused on barriers and enablers to implementing guideline recommendations about management of: upper limb sensory impairments, mobility including sitting balance; vision; anxiety and depression; neglect; swallowing; communication; education for stroke survivors and carers; advice about return to work and driving. Qualitative data were analysed for themes using theoretical domains described by Michie and colleagues (2005). Six group and two individual interviews were conducted, involving six disciplines. Barriers were different across disciplines. The six key barriers identified were: (1) Beliefs about capabilities of individual professionals and their discipline, and about patient capabilities (2) Beliefs about the consequences, positive and negative, of implementing the recommendations (3) Memory of, and attention to, best practices (4) Knowledge and skills required to implement best practice; (5) Intention and motivation to implement best practice, and (6) Resources. Some barriers were also enablers to change. For example, occupational therapists required new knowledge and skills (a barrier), to better manage sensation and neglect impairments while physiotherapists

Endogenous WNT Signals Mediate BMP-Induced and Spontaneous Differentiation of Epiblast Stem Cells and Human Embryonic Stem Cells

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Dorota Kurek

2015-01-01

Full Text Available Therapeutic application of human embryonic stem cells (hESCs requires precise control over their differentiation. However, spontaneous differentiation is prevalent, and growth factors induce multiple cell types; e.g., the mesoderm inducer BMP4 generates both mesoderm and trophoblast. Here we identify endogenous WNT signals as BMP targets that are required and sufficient for mesoderm induction, while trophoblast induction is WNT independent, enabling the exclusive differentiation toward either lineage. Furthermore, endogenous WNT signals induce loss of pluripotency in hESCs and their murine counterparts, epiblast stem cells (EpiSCs. WNT inhibition obviates the need to manually remove differentiated cells to maintain cultures and improves the efficiency of directed differentiation. In EpiSCs, WNT inhibition stabilizes a pregastrula epiblast state with novel characteristics, including the ability to contribute to blastocyst chimeras. Our findings show that endogenous WNT signals function as hidden mediators of growth factor-induced differentiation and play critical roles in the self-renewal of hESCs and EpiSCs.

Myostatin acts as an autocrine/paracrine negative regulator in myoblast differentiation from human induced pluripotent stem cells.

Science.gov (United States)

Gao, Fei; Kishida, Tsunao; Ejima, Akika; Gojo, Satoshi; Mazda, Osam

2013-02-08

Myostatin, also known as growth differentiation factor (GDF-8), regulates proliferation of muscle satellite cells, and suppresses differentiation of myoblasts into myotubes via down-regulation of key myogenic differentiation factors including MyoD. Recent advances in stem cell biology have enabled generation of myoblasts from pluripotent stem cells, but it remains to be clarified whether myostatin is also involved in regulation of artificial differentiation of myoblasts from pluripotent stem cells. Here we show that the human induced pluripotent stem (iPS) cell-derived cells that were induced to differentiate into myoblasts expressed myostatin and its receptor during the differentiation. An addition of recombinant human myostatin (rhMyostatin) suppressed induction of MyoD and Myo5a, resulting in significant suppression of myoblast differentiation. The rhMyostatin treatment also inhibited proliferation of the cells at a later phase of differentiation. RNAi-mediated silencing of myostatin promoted differentiation of human iPS-derived embryoid body (EB) cells into myoblasts. These results strongly suggest that myostatin plays an important role in regulation of myoblast differentiation from iPS cells of human origin. The present findings also have significant implications for potential regenerative medicine for muscular diseases. Copyright © 2013 Elsevier Inc. All rights reserved.

Summary of Research on Online and Blended Learning Programs That Offer Differentiated Learning Options. REL 2017-228

Science.gov (United States)

Brodersen, R. Marc; Melluzzo, Daniel

2017-01-01

This report summarizes the methodology, measures, and findings of research on the influence on student achievement outcomes of K-12 online and blended face-to-face and online learning programs that offer differentiated learning options. The report also describes the characteristics of the learning programs. Most of the examined programs used…

The micro-habitat methodology. Application protocols

Energy Technology Data Exchange (ETDEWEB)

Sabaton, C; Valentin, S; Souchon, Y

1995-06-01

A strong need has been felt for guidelines to help various entities in applying the micro-habitat methodology, particularly in impact studies on hydroelectric installations. CEMAGREF and Electricite de France have developed separately two protocols with five major steps: reconnaissance of the river, selection of representative units to be studied in greater depth, morpho-dynamic measurements at one or more rates of discharge and hydraulic modeling, coupling of hydraulic and biological models, calculation of habitat-quality scores for fish, analysis of results. The two approaches give very comparable results and are essentially differentiated by the hydraulic model used. CEMAGREF uses a one-dimensional model requiring measurements at only one discharge rate. Electricite de France uses a simplified model based on measurements at several rates of discharge. This approach is possible when discharge can be controlled in the study area during data acquisition, as is generally the case downstream of hydroelectric installations. The micro-habitat methodology is now a fully operational tool with which to study changes in fish habitat quality in relation to varying discharge. It provides an element of assessment pertinent to the choice of instreaming flow to be maintained downstream of a hydroelectric installation; this information is essential when the flow characteristics (velocity, depth) and the nature of the river bed are the preponderant factors governing habitat suitability for trout or salmon. The ultimate decision must nonetheless take into account any other potentially limiting factors for the biocenoses on the one hand, and the target water use objectives on the other. In many cases, compromises must be found among different uses, different species and different stages in the fish development cycle. (Abstract Truncated)

Confocal Raman microscopy to monitor extracellular matrix during dental pulp stem cells differentiation

Science.gov (United States)

Salehi, Hamideh; Collart-Dutilleul, Pierre-Yves; Gergely, Csilla; Cuisinier, Frédéric J. G.

2015-07-01

Regenerative medicine brings promising applications for mesenchymal stem cells, such as dental pulp stem cells (DPSCs). Confocal Raman microscopy, a noninvasive technique, is used to study osteogenic differentiation of DPSCs. Integrated Raman intensities in the 2800 to 3000 cm-1 region (C-H stretching) and the 960 cm-1 peak (ν1 PO43-) were collected (to image cells and phosphate, respectively), and the ratio of two peaks 1660 over 1690 cm-1 (amide I bands) to measure the collagen cross-linking has been calculated. Raman spectra of DPSCs after 21 days differentiation reveal several phosphate peaks: ν1 (first stretching mode) at 960 cm-1, ν2 at 430 cm-1, and ν4 at 585 cm-1 and collagen cross-linking can also be calculated. Confocal Raman microscopy enables monitoring osteogenic differentiation in vitro and can be a credible tool for clinical stem cell based research.

Day-ahead distributed energy resource scheduling using differential search algorithm

DEFF Research Database (Denmark)

Soares, J.; Lobo, C.; Silva, M.

2015-01-01

The number of dispersed energy resources is growing every day, such as the use of more distributed generators. This paper deals with energy resource scheduling model in future smart grids. The methodology can be used by virtual power players (VPPs) considering day-ahead time horizon. This method...... considers that energy resources are managed by a VPP which establishes contracts with their owners. The full AC power flow calculation included in the model takes into account network constraints. This paper presents an application of differential search algorithm (DSA) for solving the day-ahead scheduling...

Using Partial Differential Equations for Pricing of Goods and Services

Directory of Open Access Journals (Sweden)

Traykov Metodi

2016-06-01

Full Text Available This article is based on the methodology of comparative analysis, using an innovative approach for pricing of various goods and services. Benchmarking is the continuous search to find and adapt better pricing methods that leading to increased profits. We will consider the numerical solution of partial differential equations, based on Black-Scholes model for pricing of goods and services within European option. Also, we will present formulation and numerical behavior of explicit and implicit methods that can be use in pricing for company assets within European option.

Solving differential equations with unknown constitutive relations as recurrent neural networks

Energy Technology Data Exchange (ETDEWEB)

Hagge, Tobias J.; Stinis, Panagiotis; Yeung, Enoch H.; Tartakovsky, Alexandre M.

2017-12-08

We solve a system of ordinary differential equations with an unknown functional form of a sink (reaction rate) term. We assume that the measurements (time series) of state variables are partially available, and use a recurrent neural network to “learn” the reaction rate from this data. This is achieved by including discretized ordinary differential equations as part of a recurrent neural network training problem. We extend TensorFlow’s recurrent neural network architecture to create a simple but scalable and effective solver for the unknown functions, and apply it to a fedbatch bioreactor simulation problem. Use of techniques from recent deep learning literature enables training of functions with behavior manifesting over thousands of time steps. Our networks are structurally similar to recurrent neural networks, but differ in purpose, and require modified training strategies.

On methodology

DEFF Research Database (Denmark)

Cheesman, Robin; Faraone, Roque

2002-01-01

This is an English version of the methodology chapter in the authors' book "El caso Berríos: Estudio sobre información errónea, desinformación y manipulación de la opinión pública".......This is an English version of the methodology chapter in the authors' book "El caso Berríos: Estudio sobre información errónea, desinformación y manipulación de la opinión pública"....

Exploring embodied methodologies for transformative practice in early childhood and youth

Directory of Open Access Journals (Sweden)

Cahill Helen

2016-06-01

Full Text Available The development of gendered identities during early childhood and youth occurs in a context of ‘body culture’ and the hyper-visibility of ‘perfect’ bodies, which align with traditional gender ideals. Embodied methods can assist to make complexity more visible, and to allow participants to see fluidity, shifts, and becoming. Whilst there has been significant theoretical development, further methodological innovations are needed to enable children and youth to articulate their perceptions of the way multiple influences shape their relations with their own bodies. Informed by ‘new materialist’ feminist theory this article will examine the work of Australian educators exploring use of creative and embodied drama-based play. The chapter advances methodologies to support pedagogical engagement with young children and youth about gender, identity and social change. The authors explore how embodied creative play can be used across ages to support children and young people to articulate the ways social norms and expectations influence their desires, imaginings, fears and actions and their perceptions of what is possible, desirable or appropriate in relation to performances of gender in their everyday worlds.

Methodology to classify accident sequences of an Individual Plant Examination according to the severe releases for BWR type reactors

International Nuclear Information System (INIS)

Sandoval V, S.

2001-01-01

The Light Water Reactor (LWR) operation regulations require to every operating plant to perform of an Individual Plant Examination study (Ipe). One of the main purposes of an Ipe is t o gain a more quantitative understanding of the overall probabilities of core damage and fission product releases . Probabilistic Safety Analysis (PSA) methodologies and Severe Accident Analysis are used to perform Ipe studies. PSA methodologies are used to identify and analyse the set of event sequences that might originate the fission product release from a nuclear power plant; these methodologies are combinatorial in nature and generate thousands of sequences. Among other uses within an Ipe, severe accident simulations are used to determine the characteristics of the fission product release for the identified sequences and in this way, the releases can be understood and characterized. A vast amount of resources is required to simulate and analyse every Ipe sequence. This effort is unnecessary if similar sequences are grouped. The grouping scheme must achieve an efficient trade off between problem reduction and accuracy. The methodology presented in this work enables an accurate characterization and analysis of the Ipe fission product releases by using a reduced problem. The methodology encourages the use of specific plant simulations. (Author)

An Analysis of 1/f2 Phase Noise in Bipolar Colpitts Oscillators (With a Digression on Bipolar Differential-Pair LC Oscillators)

DEFF Research Database (Denmark)

Fard, A.; Andreani, Pietro

2007-01-01

This work presents an analysis of phase noise in the $1/f^{2}$ region displayed by both single-ended and differential bipolar Colpitts oscillators. Very accurate and rigorous symbolic phase noise expressions are derived, enabling a deeper insight into the major mechanisms of phase noise generatio......, and providing new tools for design optimization. Phase noise expressions for the cross-coupled differential-pair LC-tank oscillator are derived as well....

GSK-3 Inhibition Sensitizes Acute Myeloid Leukemia Cells to 1,25D-Mediated Differentiation

Science.gov (United States)

Gupta, Kalpana; Stefan, Tammy; Ignatz-Hoover, James; Moreton, Stephen; Parizher, Gary; Saunthararajah, Yogen; Wald, David N.

2017-01-01

1,25-dihydroxyvitamin D3 (1,25D), the biologically active form of vitamin D, is widely considered a promising therapy for acute myeloid leukemia (AML) based on its ability to drive differentiation of leukemic cells. However, clinical trials have been disappointing in part to dose-limiting hypercalcemia. Here we show how inhibiting glycogen synthase kinase 3 (GSK3) can improve the differentiation response of AML cells to 1,25D-mediated differentiation. GSK3 inhibition in AML cells enhanced the differentiating effects of low concentrations of 1,25D. In addition, GSK3 inhibition augmented the ability of 1,25D to induce irreversible growth inhibition and slow the progression of AML in mouse models. Mechanistic studies revealed that GSK3 inhibition led to the hyperphosphorylation of the vitamin D receptor (VDR), enabling an interaction between VDR and the coactivator, SRC-3 (NCOA3), thereby increasing transcriptional activity. We also found that activation of JNK-mediated pathways in response to GSK3 inhibition contributed to the potentiation of 1,25D-induced differentiation. Taken together, our findings offer a preclinical rationale to explore the repositioning of GSK3 inhibitors to enhance differentiation-based therapy for AML treatment. PMID:26964622

An Internet enabled impact limiter material database

Energy Technology Data Exchange (ETDEWEB)

Wix, S.; Kanipe, F.; McMurtry, W.

1998-09-01

This paper presents a detailed explanation of the construction of an interest enabled database, also known as a database driven web site. The data contained in the internet enabled database are impact limiter material and seal properties. The technique used in constructing the internet enabled database presented in this paper are applicable when information that is changing in content needs to be disseminated to a wide audience.

An Internet enabled impact limiter material database

International Nuclear Information System (INIS)

Wix, S.; Kanipe, F.; McMurtry, W.

1998-01-01

This paper presents a detailed explanation of the construction of an interest enabled database, also known as a database driven web site. The data contained in the internet enabled database are impact limiter material and seal properties. The technique used in constructing the internet enabled database presented in this paper are applicable when information that is changing in content needs to be disseminated to a wide audience

Quantitative proteomics and systems analysis of cultured H9C2 cardiomyoblasts during differentiation over time supports a 'function follows form' model of differentiation.

Science.gov (United States)

Kankeu, Cynthia; Clarke, Kylie; Van Haver, Delphi; Gevaert, Kris; Impens, Francis; Dittrich, Anna; Roderick, H Llewelyn; Passante, Egle; Huber, Heinrich J

2018-05-17

The rat cardiomyoblast cell line H9C2 has emerged as a valuable tool for studying cardiac development, mechanisms of disease and toxicology. We present here a rigorous proteomic analysis that monitored the changes in protein expression during differentiation of H9C2 cells into cardiomyocyte-like cells over time. Quantitative mass spectrometry followed by gene ontology (GO) enrichment analysis revealed that early changes in H9C2 differentiation are related to protein pathways of cardiac muscle morphogenesis and sphingolipid synthesis. These changes in the proteome were followed later in the differentiation time-course by alterations in the expression of proteins involved in cation transport and beta-oxidation. Studying the temporal profile of the H9C2 proteome during differentiation in further detail revealed eight clusters of co-regulated proteins that can be associated with early, late, continuous and transient up- and downregulation. Subsequent reactome pathway analysis based on these eight clusters further corroborated and detailed the results of the GO analysis. Specifically, this analysis confirmed that proteins related to pathways in muscle contraction are upregulated early and transiently, and proteins relevant to extracellular matrix organization are downregulated early. In contrast, upregulation of proteins related to cardiac metabolism occurs at later time points. Finally, independent validation of the proteomics results by immunoblotting confirmed hereto unknown regulators of cardiac structure and ionic metabolism. Our results are consistent with a 'function follows form' model of differentiation, whereby early and transient alterations of structural proteins enable subsequent changes that are relevant to the characteristic physiology of cardiomyocytes.

Dark field differential dynamic microscopy enables accurate characterization of the roto-translational dynamics of bacteria and colloidal clusters

Science.gov (United States)

Cerbino, Roberto; Piotti, Davide; Buscaglia, Marco; Giavazzi, Fabio

2018-01-01

Micro- and nanoscale objects with anisotropic shape are key components of a variety of biological systems and inert complex materials, and represent fundamental building blocks of novel self-assembly strategies. The time scale of their thermal motion is set by their translational and rotational diffusion coefficients, whose measurement may become difficult for relatively large particles with small optical contrast. Here we show that dark field differential dynamic microscopy is the ideal tool for probing the roto-translational Brownian motion of anisotropic shaped particles. We demonstrate our approach by successful application to aqueous dispersions of non-motile bacteria and of colloidal aggregates of spherical particles.

Diffany: an ontology-driven framework to infer, visualise and analyse differential molecular networks.

Science.gov (United States)

Van Landeghem, Sofie; Van Parys, Thomas; Dubois, Marieke; Inzé, Dirk; Van de Peer, Yves

2016-01-05

Differential networks have recently been introduced as a powerful way to study the dynamic rewiring capabilities of an interactome in response to changing environmental conditions or stimuli. Currently, such differential networks are generated and visualised using ad hoc methods, and are often limited to the analysis of only one condition-specific response or one interaction type at a time. In this work, we present a generic, ontology-driven framework to infer, visualise and analyse an arbitrary set of condition-specific responses against one reference network. To this end, we have implemented novel ontology-based algorithms that can process highly heterogeneous networks, accounting for both physical interactions and regulatory associations, symmetric and directed edges, edge weights and negation. We propose this integrative framework as a standardised methodology that allows a unified view on differential networks and promotes comparability between differential network studies. As an illustrative application, we demonstrate its usefulness on a plant abiotic stress study and we experimentally confirmed a predicted regulator. Diffany is freely available as open-source java library and Cytoscape plugin from http://bioinformatics.psb.ugent.be/supplementary_data/solan/diffany/.

Geometric methods of global attraction in systems of delay differential equations

Science.gov (United States)

El-Morshedy, Hassan A.; Ruiz-Herrera, Alfonso

2017-11-01

In this paper we deduce criteria of global attraction in systems of delay differential equations. Our methodology is new and consists in "dominating" the nonlinear terms of the system by a scalar function and then studying some dynamical properties of that function. One of the crucial benefits of our approach is that we obtain delay-dependent results of global attraction that cover the best delay-independent conditions. We apply our results in a gene regulatory model and the classical Nicholson's blowfly equation with patch structure.

Acceleration-based methodology to assess the blast mitigation performance of explosive ordnance disposal helmets

Science.gov (United States)

Dionne, J. P.; Levine, J.; Makris, A.

2018-01-01

To design the next generation of blast mitigation helmets that offer increasing levels of protection against explosive devices, manufacturers must be able to rely on appropriate test methodologies and human surrogates that will differentiate the performance level of various helmet solutions and ensure user safety. Ideally, such test methodologies and associated injury thresholds should be based on widely accepted injury criteria relevant within the context of blast. Unfortunately, even though significant research has taken place over the last decade in the area of blast neurotrauma, there currently exists no agreement in terms of injury mechanisms for blast-induced traumatic brain injury. In absence of such widely accepted test methods and injury criteria, the current study presents a specific blast test methodology focusing on explosive ordnance disposal protective equipment, involving the readily available Hybrid III mannequin, initially developed for the automotive industry. The unlikely applicability of the associated brain injury criteria (based on both linear and rotational head acceleration) is discussed in the context of blast. Test results encompassing a large number of blast configurations and personal protective equipment are presented, emphasizing the possibility to develop useful correlations between blast parameters, such as the scaled distance, and mannequin engineering measurements (head acceleration). Suggestions are put forward for a practical standardized blast testing methodology taking into account limitations in the applicability of acceleration-based injury criteria as well as the inherent variability in blast testing results.

5G-Enabled Tactile Internet

OpenAIRE

Simsek, Meryem; Aijaz, Adnan; Dohler, Mischa; Sachs, Joachim; Fettweis, Gerhard

2016-01-01

The long-term ambition of the Tactile Internet is to enable a democratization of skill, and how it is being delivered globally. An integral part of this is to be able to transmit touch in perceived real-time, which is enabled by suitable robotics and haptics equipment at the edges, along with an unprecedented communications network. The fifth generation (5G) mobile communications systems will underpin this emerging Internet at the wireless edge. This paper presents the most important technolo...

An internet enabled impact limiter material database

Energy Technology Data Exchange (ETDEWEB)

Wix, S.; Kanipe, F.; McMurtry, W. [Sandia National Labs., Albuquerque, NM (United States)

1998-07-01

This paper presents a detailed explanation of the construction of an internet enabled database, also known as a database driven web site. The data contained in the internet enabled database are impact limiter material and seal properties. The techniques used in constructing the internet enabled database presented in this paper are applicable when information that is changing in content needs to be disseminated to a wide audience. (authors)

An internet enabled impact limiter material database

International Nuclear Information System (INIS)

Wix, S.; Kanipe, F.; McMurtry, W.

1998-01-01

This paper presents a detailed explanation of the construction of an internet enabled database, also known as a database driven web site. The data contained in the internet enabled database are impact limiter material and seal properties. The techniques used in constructing the internet enabled database presented in this paper are applicable when information that is changing in content needs to be disseminated to a wide audience. (authors)

Myostatin acts as an autocrine/paracrine negative regulator in myoblast differentiation from human induced pluripotent stem cells

Energy Technology Data Exchange (ETDEWEB)

Gao, Fei; Kishida, Tsunao; Ejima, Akika [Department of Immunology, Kyoto Prefectural University of Medicine, Kyoto (Japan); Gojo, Satoshi [Department of Cardiac Support, Kyoto Prefectural University of Medicine, Kyoto (Japan); Mazda, Osam, E-mail: mazda@koto.kpu-m.ac.jp [Department of Immunology, Kyoto Prefectural University of Medicine, Kyoto (Japan)

2013-02-08

Highlights: ► iPS-derived cells express myostatin and its receptor upon myoblast differentiation. ► Myostatin inhibits myoblast differentiation by inhibiting MyoD and Myo5a induction. ► Silencing of myostatin promotes differentiation of human iPS cells into myoblasts. -- Abstract: Myostatin, also known as growth differentiation factor (GDF-8), regulates proliferation of muscle satellite cells, and suppresses differentiation of myoblasts into myotubes via down-regulation of key myogenic differentiation factors including MyoD. Recent advances in stem cell biology have enabled generation of myoblasts from pluripotent stem cells, but it remains to be clarified whether myostatin is also involved in regulation of artificial differentiation of myoblasts from pluripotent stem cells. Here we show that the human induced pluripotent stem (iPS) cell-derived cells that were induced to differentiate into myoblasts expressed myostatin and its receptor during the differentiation. An addition of recombinant human myostatin (rhMyostatin) suppressed induction of MyoD and Myo5a, resulting in significant suppression of myoblast differentiation. The rhMyostatin treatment also inhibited proliferation of the cells at a later phase of differentiation. RNAi-mediated silencing of myostatin promoted differentiation of human iPS-derived embryoid body (EB) cells into myoblasts. These results strongly suggest that myostatin plays an important role in regulation of myoblast differentiation from iPS cells of human origin. The present findings also have significant implications for potential regenerative medicine for muscular diseases.

Myostatin acts as an autocrine/paracrine negative regulator in myoblast differentiation from human induced pluripotent stem cells

International Nuclear Information System (INIS)

Gao, Fei; Kishida, Tsunao; Ejima, Akika; Gojo, Satoshi; Mazda, Osam

2013-01-01

Highlights: ► iPS-derived cells express myostatin and its receptor upon myoblast differentiation. ► Myostatin inhibits myoblast differentiation by inhibiting MyoD and Myo5a induction. ► Silencing of myostatin promotes differentiation of human iPS cells into myoblasts. -- Abstract: Myostatin, also known as growth differentiation factor (GDF-8), regulates proliferation of muscle satellite cells, and suppresses differentiation of myoblasts into myotubes via down-regulation of key myogenic differentiation factors including MyoD. Recent advances in stem cell biology have enabled generation of myoblasts from pluripotent stem cells, but it remains to be clarified whether myostatin is also involved in regulation of artificial differentiation of myoblasts from pluripotent stem cells. Here we show that the human induced pluripotent stem (iPS) cell-derived cells that were induced to differentiate into myoblasts expressed myostatin and its receptor during the differentiation. An addition of recombinant human myostatin (rhMyostatin) suppressed induction of MyoD and Myo5a, resulting in significant suppression of myoblast differentiation. The rhMyostatin treatment also inhibited proliferation of the cells at a later phase of differentiation. RNAi-mediated silencing of myostatin promoted differentiation of human iPS-derived embryoid body (EB) cells into myoblasts. These results strongly suggest that myostatin plays an important role in regulation of myoblast differentiation from iPS cells of human origin. The present findings also have significant implications for potential regenerative medicine for muscular diseases

Barriers and enablers to academic health leadership.

Science.gov (United States)

Bharwani, Aleem; Kline, Theresa; Patterson, Margaret; Craighead, Peter

2017-02-06

Purpose This study sought to identify the barriers and enablers to leadership enactment in academic health-care settings. Design/methodology/approach Semi-structured interviews ( n = 77) with programme stakeholders (medical school trainees, university leaders, clinical leaders, medical scientists and directors external to the medical school) were conducted, and the responses content-analysed. Findings Both contextual and individual factors were identified as playing a role in affecting academic health leadership enactment that has an impact on programme development, success and maintenance. Contextual factors included sufficient resources allocated to the programme, opportunities for learners to practise leadership skills, a competent team around the leader once that person is in place, clear expectations for the leader and a culture that fosters open communication. Contextual barriers included highly bureaucratic structures, fear-of-failure and non-trusting cultures and inappropriate performance systems. Programmes were advised to select participants based on self-awareness, strong communication skills and an innovative thinking style. Filling specific knowledge and skill gaps, particularly for those not trained in medical school, was viewed as essential. Ineffective decision-making styles and tendencies to get involved in day-to-day activities were barriers to the development of academic health leaders. Originality/value Programmes designed to develop academic health-care leaders will be most effective if they develop leadership at all levels; ensure that the organisation's culture, structure and processes reinforce positive leadership practices; and recognise the critical role of teams in supporting its leaders.

Scrum methodology in banking environment

OpenAIRE

Strihová, Barbora

2015-01-01

Bachelor thesis "Scrum methodology in banking environment" is focused on one of agile methodologies called Scrum and description of the methodology used in banking environment. Its main goal is to introduce the Scrum methodology and outline a real project placed in a bank focused on software development through a case study, address problems of the project, propose solutions of the addressed problems and identify anomalies of Scrum in software development constrained by the banking environmen...

Developing a semantic web model for medical differential diagnosis recommendation.

Science.gov (United States)

Mohammed, Osama; Benlamri, Rachid

2014-10-01

In this paper we describe a novel model for differential diagnosis designed to make recommendations by utilizing semantic web technologies. The model is a response to a number of requirements, ranging from incorporating essential clinical diagnostic semantics to the integration of data mining for the process of identifying candidate diseases that best explain a set of clinical features. We introduce two major components, which we find essential to the construction of an integral differential diagnosis recommendation model: the evidence-based recommender component and the proximity-based recommender component. Both approaches are driven by disease diagnosis ontologies designed specifically to enable the process of generating diagnostic recommendations. These ontologies are the disease symptom ontology and the patient ontology. The evidence-based diagnosis process develops dynamic rules based on standardized clinical pathways. The proximity-based component employs data mining to provide clinicians with diagnosis predictions, as well as generates new diagnosis rules from provided training datasets. This article describes the integration between these two components along with the developed diagnosis ontologies to form a novel medical differential diagnosis recommendation model. This article also provides test cases from the implementation of the overall model, which shows quite promising diagnostic recommendation results.

Generalized differential transform method to differential-difference equation

International Nuclear Information System (INIS)

Zou Li; Wang Zhen; Zong Zhi

2009-01-01

In this Letter, we generalize the differential transform method to solve differential-difference equation for the first time. Two simple but typical examples are applied to illustrate the validity and the great potential of the generalized differential transform method in solving differential-difference equation. A Pade technique is also introduced and combined with GDTM in aim of extending the convergence area of presented series solutions. Comparisons are made between the results of the proposed method and exact solutions. Then we apply the differential transform method to the discrete KdV equation and the discrete mKdV equation, and successfully obtain solitary wave solutions. The results reveal that the proposed method is very effective and simple. We should point out that generalized differential transform method is also easy to be applied to other nonlinear differential-difference equation.

'Ethos' Enabling Organisational Knowledge Creation

Science.gov (United States)

Matsudaira, Yoshito

This paper examines knowledge creation in relation to improvements on the production line in the manufacturing department of Nissan Motor Company and aims to clarify embodied knowledge observed in the actions of organisational members who enable knowledge creation will be clarified. For that purpose, this study adopts an approach that adds a first, second, and third-person's viewpoint to the theory of knowledge creation. Embodied knowledge, observed in the actions of organisational members who enable knowledge creation, is the continued practice of 'ethos' (in Greek) founded in Nissan Production Way as an ethical basis. Ethos is knowledge (intangible) assets for knowledge creating companies. Substantiated analysis classifies ethos into three categories: the individual, team and organisation. This indicates the precise actions of the organisational members in each category during the knowledge creation process. This research will be successful in its role of showing the indispensability of ethos - the new concept of knowledge assets, which enables knowledge creation -for future knowledge-based management in the knowledge society.

Profit, productivity, and price differential: an international performance comparison of the natural gas transportation industry

International Nuclear Information System (INIS)

Jeong-Dong Lee; Sung-Bae Park; Tai-Yoo Kim

1999-01-01

The objective of this paper is to evaluate the performance of natural gas transportation utilities, focusing on the three-key strategic performance measures of profit, productivity, and price differential. We propose a methodology that expresses the three performance measures in a unified single equation using the Edgeworth index. The proposed methodology is applied to an international comparison of the performance of 28 natural gas transportation utilities operating in eight countries in which the business environments differ greatly. The empirical results show the possible causes of performance differences and may shed some light on the direction of regulatory policy, especially for developing countries that have relatively short histories in the natural gas industry, such as Korea. (author)

Making Trade-Offs Visible: Theoretical and Methodological Considerations about the Relationship between Dimensions and Institutions of Democracy and Empirical Findings

Directory of Open Access Journals (Sweden)

Hans-Joachim Lauth

2018-03-01

Full Text Available Whereas the measurement of the quality of democracy focused on the rough differentiation of democracies and autocracies in the beginning (e.g. Vanhanen, Polity, Freedom House, the focal point of newer instruments is the assessment of the quality of established democracies. In this context, tensions resp. trade-offs between dimensions of democracy are discussed as well (e.g. Democracy Barometer, Varieties of Democracy. However, these approaches lack a systematic discussion of trade-offs and they are not able to show trade-offs empirically. We address this research desideratum in a three-step process: Firstly, we propose a new conceptual approach, which distinguishes between two different modes of relationships between dimensions: mutual reinforcing effects and a give-and-take relationship (trade-offs between dimensions. By introducing our measurement tool, Democracy Matrix, we finally locate mutually reinforcing effects as well as trade-offs. Secondly, we provide a new methodological approach to measure trade-offs. While one measuring strategy captures the mutual reinforcing effects, the other strategy employs indicators, which serve to gauge trade-offs. Thirdly, we demonstrate empirical findings of our measurement drawing on the Varieties of Democracy dataset. Incorporating trade-offs into the measurement enables us to identify various profiles of democracy (libertarian, egalitarian and control-focused democracy via the quality of its dimensions.

Development of a methodology for monthly forecasting of surface fires of Colombia's vegetation cover, an application to north Andean region

International Nuclear Information System (INIS)

Gonzalez Hernandez, Yolanda; Rangel CH, Jesus Orlando

2004-01-01

In the present article a methodology is presented for the forecasting of the monthly risk of surface fires of the vegetation cover in Colombia, based on the analysis of meteorological components and variables of climatic and anthropic variability involved in fire risks of the north Andean region. The methodology enables one to regionalize the country, with fire prediction purposes in mind, into ten sub-regions, in each one of which seven height levels are defined to make up separate regions of study. For each of these, a database is built to feed both the logistic regression models and the Poisson models, which identify the variables independent from, and/or associated with the presence or absence of fires

Experimental Economics: Some Methodological Notes

OpenAIRE

Fiore, Annamaria

2009-01-01

The aim of this work is presenting in a self-contained paper some methodological aspects as they are received in the current experimental literature. The purpose has been to make a critical review of some very influential papers dealing with methodological issues. In other words, the idea is to have a single paper where people first approaching experimental economics can find summarised (some) of the most important methodological issues. In particular, the focus is on some methodological prac...

CULTUROLOGICAL APPROACH AS METHODOLOGICAL BASIS OF MATHEMATICAL EDUCATION

Directory of Open Access Journals (Sweden)

Ye. A. Perminov

2017-01-01

literacy have the extreme humanitarian importance, since their existence either indirectly or sometimes directly influences quality of life of any person and society in general. The most in-demand, significant and obligatory thematic and methodological components of mathematical education are highlighted: mathematical modeling, discrete mathematics and computing processes. The principle of a cultural conformity and a harmonious combination of the culturological and artfundamentals of mathematical education are emphasized as the basic educational principles, the use of which is capable to improve and raise the level of mathematical culture of the Russian society on a new, higher position.The evidence from this study points towards the idea that effective functioning of the system of mathematical education is impossible without the qualified, well prepared staff who are not only professionals in the subject sphere, but also bearers of high pedagogical culture. Moral and ethical, communicative and individual, and personal components of pedagogical culture of a teacher-mathematician are characterized.Practical significance. The author is convinced that introduction of the proposed concept of mathematical education based on culturological approach to its contents and the organization will help to overcome the disproportions existing today in mathematical education between integration and subject differentiation of a training material, technologization of educational process and preservation of traditional methods of training, fundamentalization of knowledge and competence-based approach to it, etc.Materials of the publication can be useful for future and practising teachers of mathematics and allied sciences, as well as for other categories of the educators engaged in the organization and advance of mathematical education and promotion of mathematical knowledge.

Proposal of methodology of tsunami accident sequence analysis induced by earthquake using DQFM methodology

International Nuclear Information System (INIS)

Muta, Hitoshi; Muramatsu, Ken

2017-01-01

Since the Fukushima-Daiichi nuclear power station accident, the Japanese regulatory body has improved and upgraded the regulation of nuclear power plants, and continuous effort is required to enhance risk management in the mid- to long term. Earthquakes and tsunamis are considered as the most important risks, and the establishment of probabilistic risk assessment (PRA) methodologies for these events is a major issue of current PRA. The Nuclear Regulation Authority (NRA) addressed the PRA methodology for tsunamis induced by earthquakes, which is one of the methodologies that should be enhanced step by step for the improvement and maturity of PRA techniques. The AESJ standard for the procedure of seismic PRA for nuclear power plants in 2015 provides the basic concept of the methodology; however, details of the application to the actual plant PRA model have not been sufficiently provided. This study proposes a detailed PRA methodology for tsunamis induced by earthquakes using the DQFM methodology, which contributes to improving the safety of nuclear power plants. Furthermore, this study also states the issues which need more research. (author)

Culture on fibrin matrices maintains the colony-forming capacity and osteoblastic differentiation of mesenchymal stem cells

International Nuclear Information System (INIS)

Colley, Helen; McArthur, Sally L; Stolzing, Alexandra; Scutt, Andy

2012-01-01

Mesenchymal stem cells (MSC) are multipotent cells capable of differentiating into a number of mesenchymal tissues including bone, cartilage, and tendon. Low numbers in vivo means exponential growth is needed in culture to enable therapeutic applications. MSC can expand rapidly in culture but usually lose their extensive capacity for differentiation that makes them therapeutically attractive. To try and maintain their capacity for differentiation and expansion in vitro, we cultured MSC on fibrin gels of different concentrations to create more physiological growth conditions for the cells. The cells were then re-plated onto tissue culture plastic and analysed. The cells that had been pre-cultured for seven days on fibrin, proliferated and maintained their differential potential to the osteogenic lineage better than tissue culture plastic expanded MSC. A concentration relationship between colony number and fibrin concentration was seen with decreasing numbers as fibrin concentration increased. These data support the concept that substrate signals significantly influence MSC growth and differentiation and that growth on a fibrin matrix could be used to maintain a stem cell phenotype during MSC expansion. (paper)

Poorly-differentiated colorectal neuroendocrine tumour: CT differentiation from well-differentiated neuroendocrine tumour and poorly-differentiated adenocarcinomas

Energy Technology Data Exchange (ETDEWEB)

Kang, Ji Hee [Seoul National University Hospital, Department of Radiology, Seoul (Korea, Republic of); Kim, Se Hyung [Seoul National University Hospital, Department of Radiology, Seoul (Korea, Republic of); Seoul National University College of Medicine, Department of Radiology, Seoul (Korea, Republic of); Han, Joon Koo [Seoul National University Hospital, Department of Radiology, Seoul (Korea, Republic of); Seoul National University College of Medicine, Department of Radiology, Seoul (Korea, Republic of); Seoul National University Medical Research Center, Institute of Radiation Medicine, Seoul (Korea, Republic of)

2017-09-15

The differentiation of poorly-differentiated neuroendocrine tumours (PD-NETs), well-differentiated NETs (WD-NETs), and adenocarcinomas (ADCs) is important due to different management options and prognoses. This study is to find the differential CT features of colorectal PD-NETs from WD-NETs and ADCs. CT features of 25 colorectal WD-NETs, 36 PD-NETs, and 36 ADCs were retrospectively reviewed. Significant variables were assessed using univariate and multivariate analyses. Receiver operating characteristics analysis determined the optimal cut-off value of tumour and lymph node (LN) size. Large size, rectum location, ulceroinfiltrative morphology without intact overlying mucosa, heterogeneous attenuation with necrosis, presence of ≥3 enlarged LNs, and metastasis were significant variables to differentiate PD-NETs from WD-NETs (P < 0.05). High attenuation on arterial phase, persistently high enhancement pattern, presence of ≥6 enlarged LNs, large LN size, and wash-in/wash-out enhancement pattern of liver metastasis were significant variables to differentiate PD-NETs from ADCs (P < 0.05). Compared to WD-NETs, colorectal PD-NETs are usually large, heterogeneous, and ulceroinfiltrative mass without intact overlying mucosa involving enlarged LNs and metastasis. High attenuation on arterial phase, presence of enlarged LNs with larger size and greater number, and wash-in/wash-out enhancement pattern of liver metastasis can be useful CT discriminators of PD-NETs from ADCs. (orig.)

Analysis of Methodologies for Identifying Exclusion Zones for Concentrating Solar Power (CSP); Analisis de Metodologias de Identificacion de Zonas de Exclusion para Estudios de Potencial de Energia Electrica Termosolar (CSP)

Energy Technology Data Exchange (ETDEWEB)

Dominguez, P.; Ramirez, L.; Navarro, A. A.; Polo, J.; Zarza, E.

2013-07-01

The aim of this study is the proposal of a valid and unique methodology to any territory of the potential for solar power generation, reducing subjectivity and enabling comparison of results from the examination of several existing methodologies for CSP, particularly those developed by the Institute for diversification and saving of Energy (IDAE), Greenpeace, National renewable energy laboratory (NREL) and the German Aerospace Center (DLR). Subsequently, we apply and compare the results obtained with those already installed CSP plants, giving an idea of the suitability of each methodology to locate plants in areas considered suitable. (Author)

Pragmatic critical realism: could this methodological approach expand our understanding of employment relations?

Science.gov (United States)

Mearns, Susan Lesley

2011-01-01

This paper seeks to highlight the need for employment relations academics and researchers to expand their use of research methodologies in order for them to enable the advancement of theoretical debate within their discipline. It focuses on the contribution that pragmatical critical realism has made to the field of perception and argues that it would add value to the subject of employment relations. It is a theoretically centred review of pragmatical critical realism and the possible contribution this methodology would make to the field of employment relations. The paper concludes that the employment relationship does not take place in a vacuum rather it is focussed on the interaction between imperfect individuals. Therefore, their interactions are moulded by emotions which can not be explored thoroughly or even acknowledged through a positivists' rigorous but limited acknowledgment of what constitutes 'knowledge' and development of theory. While not rejecting the contribution that quantitative data or positivism have made to the field, the study concludes that pragmatic critical realism has a lot to offer the development of the area and its theoretical foundations.

LOFT uncertainty-analysis methodology

International Nuclear Information System (INIS)

Lassahn, G.D.

1983-01-01

The methodology used for uncertainty analyses of measurements in the Loss-of-Fluid Test (LOFT) nuclear-reactor-safety research program is described and compared with other methodologies established for performing uncertainty analyses

LOFT uncertainty-analysis methodology

International Nuclear Information System (INIS)

Lassahn, G.D.

1983-01-01

The methodology used for uncertainty analyses of measurements in the Loss-of-Fluid Test (LOFT) nuclear reactor safety research program is described and compared with other methodologies established for performing uncertainty analyses

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells.

Science.gov (United States)

Szaraz, Peter; Gratch, Yarden S; Iqbal, Farwah; Librach, Clifford L

2017-08-09

Myocardial infarction and the subsequent ischemic cascade result in the extensive loss of cardiomyocytes, leading to congestive heart failure, the leading cause of mortality worldwide. Mesenchymal stem cells (MSCs) are a promising option for cell-based therapies to replace current, invasive techniques. MSCs can differentiate into mesenchymal lineages, including cardiac cell types, but complete differentiation into functional cells has not yet been achieved. Previous methods of differentiation were based on pharmacological agents or growth factors. However, more physiologically relevant strategies can also enable MSCs to undergo cardiomyogenic transformation. Here, we present a differentiation method using MSC aggregates on cardiomyocyte feeder layers to produce cardiomyocyte-like contracting cells. Human umbilical cord perivascular cells (HUCPVCs) have been shown to have a greater differentiation potential than commonly investigated MSC types, such as bone marrow MSCs (BMSCs). As an ontogenetically younger source, we investigated the cardiomyogenic potential of first-trimester (FTM) HUCPVCs compared to older sources. FTM HUCPVCs are a novel, rich source of MSCs that retain their in utero immunoprivileged properties when cultured in vitro. Using this differentiation protocol, FTM and term HUCPVCs achieved significantly increased cardiomyogenic differentiation compared to BMSCs, as indicated by the increased expression of cardiomyocyte markers (i.e., myocyte enhancer factor 2C, cardiac troponin T, heavy chain cardiac myosin, signal regulatory protein α, and connexin 43). They also maintained significantly lower immunogenicity, as demonstrated by their lower HLA-A expression and higher HLA-G expression. Applying aggregate-based differentiation, FTM HUCPVCs showed increased aggregate formation potential and generated contracting cells clusters within 1 week of co-culture on cardiac feeder layers, becoming the first MSC type to do so. Our results demonstrate that this

Addressing the gender differentiated investment risks to climate-smart agriculture

Directory of Open Access Journals (Sweden)

Yannick Glemarec

2017-01-01

Full Text Available This paper argues that closing the gender gap in land and other productive resources can provide a “triple dividend” of gender equality, food security and climate management, thereby offering a cost-effective approach to the pursuit of the Sustainable Development Goals. However, climate change compounds the structural barriers to gender equality that women farmers face, rapidly shrinking the window of opportunity to realize this triple dividend. Yet climate policies largely overlook the gender gap in agriculture.The growing literature on the gender gap aims to better quantify its implications for agricultural productivity but does not provide a framework to prioritize policy responses. To complement these econometric efforts, this paper proposes a three-step methodology to assist policy-makers in developing countries in disentangling the opportunities and trade-offs of different policies and interventions to close the gender gap that impedes climate-smart agriculture (CSA for women.Barrier and risk analyses are increasingly used to identify public instruments that can catalyze climate smart investments. Building on this proven methodology, the paper first develops a table that clusters barriers to CSA into nine independent risk categories. Second, it overlays a gender analysis upon this gender-neutral barrier and risk table to identify gender-differentiated risks and barriers to CSA. Third, it maps identified gender-neutral and differentiated investment risks against possible remedial public policy instruments.The analysis suggests that about half of identified CSA investment risks have a higher probability of occurrence for women farmers than for men farmers. Furthermore, women farmers might face additional gender-specific barriers, mostly linked to their disproportionate responsibility for unpaid domestic and care work, the risk of violence and unequal power relations with men in the household and community. Targeted interventions will be

The enabling approach for housing supply

Directory of Open Access Journals (Sweden)

Ghada Farouk Hassan

2011-12-01

The paper attempts to highlight prerequisites needed to improve the success of the enabling approach in achieving adequate housing provision. Then the paper revisits the Egyptian experiences in the application of the enabling approach from 2005 till 2010. Finally, the paper highlights the main drops and lessons must be considered as promising approach after the revolution.

Poincare map for some polynomial systems of differential equations

International Nuclear Information System (INIS)

Varin, V P

2004-01-01

One approach to the classical problem of distinguishing between a centre and a focus for a system of differential equations with polynomial right-hand sides in the plane is discussed. For a broad class of such systems necessary and sufficient conditions for a centre are expressed in terms of equations in variations of higher order. By contrast with the existing methods of investigation, attention is concentrated on the explicit calculation of the asymptotic behaviour of the Poincare map rather than on finding sufficient centre conditions as such; this also enables one to study bifurcations of birth of arbitrarily strongly degenerate cycles.

A moveable feast: Exploring barriers and enablers to food citizenship.

Science.gov (United States)

O'Kane, Gabrielle

2016-10-01

Despite the ability of the globalised food system to deliver varied and plentiful food supplies to most in the developed world it also disconnects consumers from where, how and by whom food is grown, which discourages food citizenship. This paper uses an ecological framework to examine the individual, social, physical and macro-level environments that can positively or negatively influence peoples' engagement with food citizenship. This research used narrative inquiry methodology and purposive sampling to gather stories through focus group conversations. Fifty-two people voluntarily attended focus groups comprised of food procurers from one of five sources: community gardens, Community Supported Agriculture (CSA), farmers' markets, fresh food markets or supermarkets. A narrative was constructed for each of these groups of food procurers; their commonalities and particularities were illuminated through a final interpretive narrative. The findings showed that the community gardeners exhibited a range of personal characteristics that prioritised engagement with local food systems. The physical setting of the community garden enabled the gardeners to make connections to the earth and the people within their social networks. The CSA and farmers' market groups were cognisant of the implications of their food choices and usually proved to be food citizens, as they selected seasonal, local and ethically produced food from their farmer(s). The fresh food market shoppers were ambivalent towards organic and seasonal food and the market setting did not connect them directly to the provenance of their food. The supermarket shoppers' disconnection from those who grew their food and where it was grown disempowered them from making sustainable food choices. Adjustments to the macro-level food environment are needed to enable people to have a greater commitment to food citizenship. Copyright © 2016 Elsevier Ltd. All rights reserved.

[ENDOSONOGRAPHY IN THE DIFFERENTIAL DIAGNOSIS OF THE COMMON BILE DUCT STENOSIS].

Science.gov (United States)

Solodinina, E N; Starkov, Y G; Shumkin, L V

2015-01-01

The article states the results of examination and treatment of 57 patients with stenosis of the common bile duct of various genesis. The main aim of the work is criteria definition and evaluation of diagnostic significance of endosonography in the differential diagnosis of benign and malignant common bile duct stenosis. The paper presents a methodology of endoscopic ultrasound and basic criteria for the differential diagnosis of tumors and other lesions of the extrahepatic bile ducts. A comparative analysis of endosonography, ultrasound, CT, MRCP was conducted. The sensitivity of endosonography in determining the nature of the common bile duct stenosis was 97.7%, a specificity 100% and accuracy 98.2%, which is superior to other methods of radiological diagnosis. In comprehensive surgical centers endosonography should be used as a method of specifying the final diagnosis to determine the nature of the common bile duct stenosis, particularly at low constriction location.

Transmission pricing: paradigms and methodologies

Energy Technology Data Exchange (ETDEWEB)

Shirmohammadi, Dariush [Pacific Gas and Electric Co., San Francisco, CA (United States); Vieira Filho, Xisto; Gorenstin, Boris [Centro de Pesquisas de Energia Eletrica (CEPEL), Rio de Janeiro, RJ (Brazil); Pereira, Mario V.P. [Power System Research, Rio de Janeiro, RJ (Brazil)

1994-12-31

In this paper we describe the principles of several paradigms and methodologies for pricing transmission services. The paper outlines some of the main characteristics of these paradigms and methodologies such as where they may be used for best results. Due to their popularity, power flow based MW-mile and short run marginal cost pricing methodologies will be covered in some detail. We conclude the paper with examples of the application of these two pricing methodologies for pricing transmission services in Brazil. (author) 25 refs., 2 tabs.

Spatial differentiation in characterisation modelling – what difference does it make?

DEFF Research Database (Denmark)

Hauschild, Michael Zwicky; Potting, José

2004-01-01

In the life cycle of a product, emissions take place at many different locations. The location of the sources and its surrounding condition influence the fate of the emission and the exposure it leads to but this source of variation is currently neglected in life cycle impact assessment, although...... results from the Danish LCA Methodology Development and Consensus Creation Project address this issue and provides a framework for spatially differentiated characterisation modelling together with easily applicable site-dependent factors for each European country and normalisation references for those...

Yeast three-hybrid screen identifies TgBRADIN/GRA24 as a negative regulator of Toxoplasma gondii bradyzoite differentiation.

Directory of Open Access Journals (Sweden)

Anahi V Odell

Full Text Available Differentiation of the protozoan parasite Toxoplasma gondii into its latent bradyzoite stage is a key event in the parasite's life cycle. Compound 2 is an imidazopyridine that was previously shown to inhibit the parasite lytic cycle, in part through inhibition of parasite cGMP-dependent protein kinase. We show here that Compound 2 can also enhance parasite differentiation, and we use yeast three-hybrid analysis to identify TgBRADIN/GRA24 as a parasite protein that interacts directly or indirectly with the compound. Disruption of the TgBRADIN/GRA24 gene leads to enhanced differentiation of the parasite, and the TgBRADIN/GRA24 knockout parasites show decreased susceptibility to the differentiation-enhancing effects of Compound 2. This study represents the first use of yeast three-hybrid analysis to study small-molecule mechanism of action in any pathogenic microorganism, and it identifies a previously unrecognized inhibitor of differentiation in T. gondii. A better understanding of the proteins and mechanisms regulating T. gondii differentiation will enable new approaches to preventing the establishment of chronic infection in this important human pathogen.

Feminist Methodologies and Engineering Education Research

Science.gov (United States)

Beddoes, Kacey

2013-01-01

This paper introduces feminist methodologies in the context of engineering education research. It builds upon other recent methodology articles in engineering education journals and presents feminist research methodologies as a concrete engineering education setting in which to explore the connections between epistemology, methodology and theory.…

Spatially enabled land administration

DEFF Research Database (Denmark)

Enemark, Stig

2006-01-01

enabling of land administration systems managing tenure, valuation, planning, and development will allow the information generated by these activities to be much more useful. Also, the services available to private and public sectors and to community organisations should commensurably improve. Knowledge....... In other words: Good governance and sustainable development is not attainable without sound land administration or - more broadly – sound land management. The paper presents a land management vision that incorporates the benefits of ICT enabled land administration functions. The idea is that spatial...... the communication between administrative systems and also establish more reliable data due to the use the original data instead of copies. In Denmark, such governmental guidelines for a service-oriented ITarchitecture in support of e-government are recently adopted. Finally, the paper presents the role of FIG...

Combined measurement of growth and differentiation in suspension cultures of purified human CD34-positive cells enables a detailed analysis of myelopoiesis

NARCIS (Netherlands)

Kerst, J. M.; Slaper-Cortenbach, I. C.; von dem Borne, A. E.; van der Schoot, C. E.; van Oers, R. H.

1992-01-01

In this study we have made a detailed analysis of growth factor (granulocyte-macrophage colony-stimulating factor [GM-CSF], granulocyte colony-stimulating factor [G-CSF], and macrophage colony-stimulating factor [M-CSF])-induced proliferation and differentiation of highly purified CD34+ committed

Analytical solutions for systems of partial differential-algebraic equations.

Science.gov (United States)

Benhammouda, Brahim; Vazquez-Leal, Hector

2014-01-01

This work presents the application of the power series method (PSM) to find solutions of partial differential-algebraic equations (PDAEs). Two systems of index-one and index-three are solved to show that PSM can provide analytical solutions of PDAEs in convergent series form. What is more, we present the post-treatment of the power series solutions with the Laplace-Padé (LP) resummation method as a useful strategy to find exact solutions. The main advantage of the proposed methodology is that the procedure is based on a few straightforward steps and it does not generate secular terms or depends of a perturbation parameter.

SNMG: a social-level norm-based methodology for macro-governing service collaboration processes

Science.gov (United States)

Gao, Ji; Lv, Hexin; Jin, Zhiyong; Xu, Ping

2017-08-01

In order to adapt to the accelerative open tendency of collaborations between enterprises, this paper proposes a Social-level Norm-based methodology for Macro-Governing service collaboration processes, called SNMG, to regulate and control the social-level visible macro-behaviors of the social individuals participating in collaborations. SNMG not only can remove effectively the uncontrollability hindrance confronted with by open social activities, but also enables across-management-domain collaborations to be implemented by uniting the centralized controls of social individuals for respective social activities. Therefore, this paper provides a brand-new system construction mode to promote the development and large-scale deployment of service collaborations.

The Concept of Post-Non-Classic al Methodological Strategy by Professor Olexander Oguy

Directory of Open Access Journals (Sweden)

Olha Chervinska

2017-12-01

a profound present-day academic research. It is worth reading for everyone, who deals with literary genres. The scientist made up his mind to tackle the problem of genres due to the ambiguity of the criteria that existed in Post-Soviet Literary Studies. He activated the notion of “synergic approach”, which enabled him to rethink drastically the whole genre system in the Middle Age literature. O. D. Oguy’s last and largest article “The Principles of Formation of the Middle Age German Literature: Overcoming the Crisis Stages through New Genres” appeared in “Problems of Literary Criticism” in 2013 (Issue 88. It impresses the reader with a broad scope application of diachronic principle in a research space, as well as with the methodology of scientific discourse (the so-called “content analysis”, which presupposes the effect of social factors in investigating the cultural phenomena. The comparison of literary artefacts with the events in European history has enabled O. Oguy to determine and classify eight crisis stages (including the prehistory. The latter had been accompanying the formation of the German nation from the early Christian days and the Great Relocation of Peoples till the end of the Middle Ages, as well as had exercised a direct influence on the changes in the literary process.

Methodology for determining the investment attractiveness of construction of high-rise buildings

Science.gov (United States)

Nezhnikova, Ekaterina; Kashirin, Valentin; Davydova, Yana; Kazakova, Svetlana

2018-03-01

The article presents the analysis of the existing methods for assessing the investment attractiveness of high-rise construction. The authors determined and justified the primary choice of objects and territories that are the most attractive for the development of high-rise construction. A system of risk indicators has been developed that allow making a quantitative adjustment for a particular project in the evaluation of the efficiency of investment projects. The study is aimed at developing basic methodological concepts for a comparative evaluation of the prospects of construction of high-rise facilities that allow to take into consideration the features of investment in construction and to enable quantitative evaluation of the investment effectiveness in high-rise construction.

Implementation and training methodology of subcritical reactors neutronic calculations triggered by external neutron source and applications

International Nuclear Information System (INIS)

Carluccio, Thiago

2011-01-01

This works had as goal to investigate calculational methodologies on subcritical source driven reactor, such as Accelerator Driven Subcritical Reactor (ADSR) and Fusion Driven Subcritical Reactor (FDSR). Intense R and D has been done about these subcritical concepts, mainly due to Minor Actinides (MA) and Long Lived Fission Products (LLFP) transmutation possibilities. In this work, particular emphasis has been given to: (1) complement and improve calculation methodology with neutronic transmutation and decay capabilities and implement it computationally, (2) utilization of this methodology in the Coordinated Research Project (CRP) of the International Atomic Energy Agency Analytical and Experimental Benchmark Analysis of ADS and in the Collaborative Work on Use of Low Enriched Uranium in ADS, especially in the reproduction of the experimental results of the Yalina Booster subcritical assembly and study of a subcritical core of IPEN / MB-01 reactor, (3) to compare different nuclear data libraries calculation of integral parameters, such as k eff and k src , and differential distributions, such as spectrum and flux, and nuclides inventories and (4) apply the develop methodology in a study that may help future choices about dedicated transmutation system. The following tools have been used in this work: MCNP (Monte Carlo N particle transport code), MCB (enhanced version of MCNP that allows burnup calculation) and NJOY to process nuclear data from evaluated nuclear data files. (author)

Formalizing the ISDF Software Development Methodology

Directory of Open Access Journals (Sweden)

Mihai Liviu DESPA

2015-01-01

Full Text Available The paper is aimed at depicting the ISDF software development methodology by emphasizing quality management and software development lifecycle. The ISDF methodology was built especially for innovative software development projects. The ISDF methodology was developed empirically by trial and error in the process of implementing multiple innovative projects. The research process began by analysing key concepts like innovation and software development and by settling the important dilemma of what makes a web application innovative. Innovation in software development is presented from the end-user, project owner and project manager’s point of view. The main components of a software development methodology are identified. Thus a software development methodology should account for people, roles, skills, teams, tools, techniques, processes, activities, standards, quality measuring tools, and team values. Current software development models are presented and briefly analysed. The need for a dedicated innovation oriented software development methodology is emphasized by highlighting shortcomings of current software development methodologies when tackling innovation. The ISDF methodology is presented in the context of developing an actual application. The ALHPA application is used as a case study for emphasizing the characteristics of the ISDF methodology. The development life cycle of the ISDF methodology includes research, planning, prototyping, design, development, testing, setup and maintenance. Artefacts generated by the ISDF methodology are presented. Quality is managed in the ISDF methodology by assessing compliance, usability, reliability, repeatability, availability and security. In order to properly asses each quality component a dedicated indicator is built. A template for interpreting each indicator is provided. Conclusions are formulated and new related research topics are submitted for debate.

The welfare costs of rent-seeking: a methodologically individualist and subjectivist revision

Directory of Open Access Journals (Sweden)

Michael Makovi

2015-11-01

Full Text Available Gordon Tullock is acknowledged for being the first to recognize the true costs of rent-seeking as including not only the Harberger triangle but also the Tullock rectangle. This rectangle does not constitute merely a lossless transfer of wealth, but it causes a misallocation of resources as rent-seekers invest resources in lobbying. However, a close reading of Tullock’s writings shows that his arguments are formulated in a holistic fashion, speaking of what is efficient or inefficient for society. Rent-seeking is inefficient because it reduces societal welfare. But according to a methodologically individualist and subjectivist economics, such a claim is invalid. We must distinguish between positive economic fact and normative moral philosophy. We call for a reconstruction of utility and welfare economics based on methodological individualism and subjectivism with implications for the theories of monopoly and competition: practices which Neoclassical perfect-competition theory considers to be evidence of rent-seeking should instead be deemed as indications of genuine competition Political economy should be concerned with ascertaining which institutions will best enable individuals to pursue their individually subjective ends – or else economists should be explicit about their normative preferences and political philosophies.

A stochastic differential equation analysis of cerebrospinal fluid dynamics.

Science.gov (United States)

Raman, Kalyan

2011-01-18

Clinical measurements of intracranial pressure (ICP) over time show fluctuations around the deterministic time path predicted by a classic mathematical model in hydrocephalus research. Thus an important issue in mathematical research on hydrocephalus remains unaddressed--modeling the effect of noise on CSF dynamics. Our objective is to mathematically model the noise in the data. The classic model relating the temporal evolution of ICP in pressure-volume studies to infusions is a nonlinear differential equation based on natural physical analogies between CSF dynamics and an electrical circuit. Brownian motion was incorporated into the differential equation describing CSF dynamics to obtain a nonlinear stochastic differential equation (SDE) that accommodates the fluctuations in ICP. The SDE is explicitly solved and the dynamic probabilities of exceeding critical levels of ICP under different clinical conditions are computed. A key finding is that the probabilities display strong threshold effects with respect to noise. Above the noise threshold, the probabilities are significantly influenced by the resistance to CSF outflow and the intensity of the noise. Fluctuations in the CSF formation rate increase fluctuations in the ICP and they should be minimized to lower the patient's risk. The nonlinear SDE provides a scientific methodology for dynamic risk management of patients. The dynamic output of the SDE matches the noisy ICP data generated by the actual intracranial dynamics of patients better than the classic model used in prior research.

Quantification of differential gene expression by multiplexed targeted resequencing of cDNA

Science.gov (United States)

Arts, Peer; van der Raadt, Jori; van Gestel, Sebastianus H.C.; Steehouwer, Marloes; Shendure, Jay; Hoischen, Alexander; Albers, Cornelis A.

2017-01-01

Whole-transcriptome or RNA sequencing (RNA-Seq) is a powerful and versatile tool for functional analysis of different types of RNA molecules, but sample reagent and sequencing cost can be prohibitive for hypothesis-driven studies where the aim is to quantify differential expression of a limited number of genes. Here we present an approach for quantification of differential mRNA expression by targeted resequencing of complementary DNA using single-molecule molecular inversion probes (cDNA-smMIPs) that enable highly multiplexed resequencing of cDNA target regions of ∼100 nucleotides and counting of individual molecules. We show that accurate estimates of differential expression can be obtained from molecule counts for hundreds of smMIPs per reaction and that smMIPs are also suitable for quantification of relative gene expression and allele-specific expression. Compared with low-coverage RNA-Seq and a hybridization-based targeted RNA-Seq method, cDNA-smMIPs are a cost-effective high-throughput tool for hypothesis-driven expression analysis in large numbers of genes (10 to 500) and samples (hundreds to thousands). PMID:28474677

Informatics in radiology: radiology gamuts ontology: differential diagnosis for the Semantic Web.

Science.gov (United States)

Budovec, Joseph J; Lam, Cesar A; Kahn, Charles E

2014-01-01

The Semantic Web is an effort to add semantics, or "meaning," to empower automated searching and processing of Web-based information. The overarching goal of the Semantic Web is to enable users to more easily find, share, and combine information. Critical to this vision are knowledge models called ontologies, which define a set of concepts and formalize the relations between them. Ontologies have been developed to manage and exploit the large and rapidly growing volume of information in biomedical domains. In diagnostic radiology, lists of differential diagnoses of imaging observations, called gamuts, provide an important source of knowledge. The Radiology Gamuts Ontology (RGO) is a formal knowledge model of differential diagnoses in radiology that includes 1674 differential diagnoses, 19,017 terms, and 52,976 links between terms. Its knowledge is used to provide an interactive, freely available online reference of radiology gamuts ( www.gamuts.net ). A Web service allows its content to be discovered and consumed by other information systems. The RGO integrates radiologic knowledge with other biomedical ontologies as part of the Semantic Web. © RSNA, 2014.

Bioinspired nanocomplex for spatiotemporal imaging of sequential mRNA expression in differentiating neural stem cells.

Science.gov (United States)

Wang, Zhe; Zhang, Ruili; Wang, Zhongliang; Wang, He-Fang; Wang, Yu; Zhao, Jun; Wang, Fu; Li, Weitao; Niu, Gang; Kiesewetter, Dale O; Chen, Xiaoyuan

2014-12-23

Messenger RNA plays a pivotal role in regulating cellular activities. The expression dynamics of specific mRNA contains substantial information on the intracellular milieu. Unlike the imaging of stationary mRNAs, real-time intracellular imaging of the dynamics of mRNA expression is of great value for investigating mRNA biology and exploring specific cellular cascades. In addition to advanced imaging methods, timely extracellular stimulation is another key factor in regulating the mRNA expression repertoire. The integration of effective stimulation and imaging into a single robust system would significantly improve stimulation efficiency and imaging accuracy, producing fewer unwanted artifacts. In this study, we developed a multifunctional nanocomplex to enable self-activating and spatiotemporal imaging of the dynamics of mRNA sequential expression during the neural stem cell differentiation process. This nanocomplex showed improved enzymatic stability, fast recognition kinetics, and high specificity. With a mechanism regulated by endogenous cell machinery, this nanocomplex realized the successive stimulating motif release and the dynamic imaging of chronological mRNA expression during neural stem cell differentiation without the use of transgenetic manipulation. The dynamic imaging montage of mRNA expression ultimately facilitated genetic heterogeneity analysis. In vivo lateral ventricle injection of this nanocomplex enabled endogenous neural stem cell activation and labeling at their specific differentiation stages. This nanocomplex is highly amenable as an alternative tool to explore the dynamics of intricate mRNA activities in various physiological and pathological conditions.

Environmental impact statement analysis: dose methodology

International Nuclear Information System (INIS)

Mueller, M.A.; Strenge, D.L.; Napier, B.A.

1981-01-01

Standardized sections and methodologies are being developed for use in environmental impact statements (EIS) for activities to be conducted on the Hanford Reservation. Five areas for standardization have been identified: routine operations dose methodologies, accident dose methodology, Hanford Site description, health effects methodology, and socioeconomic environment for Hanford waste management activities

Regional Shelter Analysis Methodology

Energy Technology Data Exchange (ETDEWEB)

Dillon, Michael B. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Dennison, Deborah [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Kane, Jave [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Walker, Hoyt [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Miller, Paul [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

2015-08-01

The fallout from a nuclear explosion has the potential to injure or kill 100,000 or more people through exposure to external gamma (fallout) radiation. Existing buildings can reduce radiation exposure by placing material between fallout particles and exposed people. Lawrence Livermore National Laboratory was tasked with developing an operationally feasible methodology that could improve fallout casualty estimates. The methodology, called a Regional Shelter Analysis, combines the fallout protection that existing buildings provide civilian populations with the distribution of people in various locations. The Regional Shelter Analysis method allows the consideration of (a) multiple building types and locations within buildings, (b) country specific estimates, (c) population posture (e.g., unwarned vs. minimally warned), and (d) the time of day (e.g., night vs. day). The protection estimates can be combined with fallout predictions (or measurements) to (a) provide a more accurate assessment of exposure and injury and (b) evaluate the effectiveness of various casualty mitigation strategies. This report describes the Regional Shelter Analysis methodology, highlights key operational aspects (including demonstrating that the methodology is compatible with current tools), illustrates how to implement the methodology, and provides suggestions for future work.

Blending critical realist and emancipatory practice development methodologies: making critical realism work in nursing research.

LENUS (Irish Health Repository)

Parlour, Randal

2012-12-01

This paper examines the efficacy of facilitation as a practice development intervention in changing practice within an Older Person setting and in implementing evidence into practice. It outlines the influences exerted by the critical realist paradigm in guiding emancipatory practice development activities and, in particular, how the former may be employed within an emancipatory practice development study to elucidate and increase understanding pertinent to causation and outcomes. The methodology is based upon an emancipatory practice development approach set within a realistic evaluation framework. This allows for systematic analysis of the social and contextual elements that influence the explication of outcomes associated with facilitation. The study is concentrated upon five practice development cycles, within which a sequence of iterative processes is integrated. The authors assert that combining critical realist and emancipatory processes offers a robust and practical method for translating evidence and implementing changes in practice, as the former affirms or falsifies the influence that emancipatory processes exert on attaining culture shift, and enabling transformation towards effective clinical practice. A new framework for practice development is proposed that establishes methodological coherency between emancipatory practice development and realistic evaluation. This augments the existing theoretical bases for both these approaches by contributing new theoretical and methodological understandings of causation.

An integrated methodology for process improvement and delivery system visualization at a multidisciplinary cancer center.

Science.gov (United States)

Singprasong, Rachanee; Eldabi, Tillal

2013-01-01

Multidisciplinary cancer centers require an integrated, collaborative, and stream-lined workflow in order to provide high quality of patient care. Due to the complex nature of cancer care and continuing changes to treatment techniques and technologies, it is a constant struggle for centers to obtain a systemic and holistic view of treatment workflow for improving the delivery systems. Project management techniques, Responsibility matrix and a swim-lane activity diagram representing sequence of activities can be combined for data collection, presentation, and evaluation of the patient care. This paper presents this integrated methodology using multidisciplinary meetings and walking the route approach for data collection, integrated responsibility matrix and swim-lane activity diagram with activity time for data representation and 5-why and gap analysis approach for data analysis. This enables collection of right detail of information in a shorter time frame by identifying process flaws and deficiencies while being independent of the nature of the patient's disease or treatment techniques. A case study of a multidisciplinary regional cancer centre is used to illustrate effectiveness of the proposed methodology and demonstrates that the methodology is simple to understand, allowing for minimal training of staff and rapid implementation. © 2011 National Association for Healthcare Quality.

Melatonin enhances neural stem cell differentiation and engraftment by increasing mitochondrial function.

Science.gov (United States)

Mendivil-Perez, Miguel; Soto-Mercado, Viviana; Guerra-Librero, Ana; Fernandez-Gil, Beatriz I; Florido, Javier; Shen, Ying-Qiang; Tejada, Miguel A; Capilla-Gonzalez, Vivian; Rusanova, Iryna; Garcia-Verdugo, José M; Acuña-Castroviejo, Darío; López, Luis Carlos; Velez-Pardo, Carlos; Jimenez-Del-Rio, Marlene; Ferrer, José M; Escames, Germaine

2017-09-01

Neural stem cells (NSCs) are regarded as a promising therapeutic approach to protecting and restoring damaged neurons in neurodegenerative diseases (NDs) such as Parkinson's disease and Alzheimer's disease (PD and AD, respectively). However, new research suggests that NSC differentiation is required to make this strategy effective. Several studies have demonstrated that melatonin increases mature neuronal markers, which reflects NSC differentiation into neurons. Nevertheless, the possible involvement of mitochondria in the effects of melatonin during NSC differentiation has not yet been fully established. We therefore tested the impact of melatonin on NSC proliferation and differentiation in an attempt to determine whether these actions depend on modulating mitochondrial activity. We measured proliferation and differentiation markers, mitochondrial structural and functional parameters as well as oxidative stress indicators and also evaluated cell transplant engraftment. This enabled us to show that melatonin (25 μM) induces NSC differentiation into oligodendrocytes and neurons. These effects depend on increased mitochondrial mass/DNA/complexes, mitochondrial respiration, and membrane potential as well as ATP synthesis in NSCs. It is also interesting to note that melatonin prevented oxidative stress caused by high levels of mitochondrial activity. Finally, we found that melatonin enriches NSC engraftment in the ND mouse model following transplantation. We concluded that a combined therapy involving transplantation of NSCs pretreated with pharmacological doses of melatonin could efficiently restore neuronal cell populations in PD and AD mouse models depending on mitochondrial activity promotion. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

[Application of Land-use Regression Models in Spatial-temporal Differentiation of Air Pollution].

Science.gov (United States)

Wu, Jian-sheng; Xie, Wu-dan; Li, Jia-cheng

2016-02-15

With the rapid development of urbanization, industrialization and motorization, air pollution has become one of the most serious environmental problems in our country, which has negative impacts on public health and ecological environment. LUR model is one of the common methods simulating spatial-temporal differentiation of air pollution at city scale. It has broad application in Europe and North America, but not really in China. Based on many studies at home and abroad, this study started with the main steps to develop LUR model, including obtaining the monitoring data, generating variables, developing models, model validation and regression mapping. Then a conclusion was drawn on the progress of LUR models in spatial-temporal differentiation of air pollution. Furthermore, the research focus and orientation in the future were prospected, including highlighting spatial-temporal differentiation, increasing classes of model variables and improving the methods of model development. This paper was aimed to popularize the application of LUR model in China, and provide a methodological basis for human exposure, epidemiologic study and health risk assessment.

Chronology of Islet Differentiation Revealed By Temporal Cell Labeling

Science.gov (United States)

Miyatsuka, Takeshi; Li, Zhongmei; German, Michael S.

2009-01-01

OBJECTIVE Neurogenin 3 plays a pivotal role in pancreatic endocrine differentiation. Whereas mouse models expressing reporters such as eGFP or LacZ under the control of the Neurog3 gene enable us to label cells in the pancreatic endocrine lineage, the long half-life of most reporter proteins makes it difficult to distinguish cells actively expressing neurogenin 3 from differentiated cells that have stopped transcribing the gene. RESEARCH DESIGN AND METHODS In order to separate the transient neurogenin 3 –expressing endocrine progenitor cells from the differentiating endocrine cells, we developed a mouse model (Ngn3-Timer) in which DsRed-E5, a fluorescent protein that shifts its emission spectrum from green to red over time, was expressed transgenically from the NEUROG3 locus. RESULTS In the Ngn3-Timer embryos, green-dominant cells could be readily detected by microscopy or flow cytometry and distinguished from green/red double-positive cells. When fluorescent cells were sorted into three different populations by a fluorescence-activated cell sorter, placed in culture, and then reanalyzed by flow cytometry, green-dominant cells converted to green/red double-positive cells within 6 h. The sorted cell populations were then used to determine the temporal patterns of expression for 145 transcriptional regulators in the developing pancreas. CONCLUSIONS The precise temporal resolution of this model defines the narrow window of neurogenin 3 expression in islet progenitor cells and permits sequential analyses of sorted cells as well as the testing of gene regulatory models for the differentiation of pancreatic islet cells. PMID:19478145

Differential-discrete mathematical model of two phase flow heat exchanger

International Nuclear Information System (INIS)

Debeljkovic, D.Lj.; Zitek, Pavel; Simeunovic, G.; Inard, Christian

2007-01-01

A dynamic thermal-hydraulic mathematical model of evaporator dynamics of a once - through sub critical steam generator is derived and presented. This model allows the investigation of evaporator dynamics including its transients responses. The evaporator was considered as a part of three-section (economizer, evaporator and super-heater) model with time varying phase boundaries and is described by a set of linearized discrete - difference equations which, with some other algebraic equations, constitutes a closed system of equations possible for exact computer solution. This model has been derived upon the fundamental equations of mass, energy and momentum balance. For the first time, a discrete differential approach has been applied in order to investigate such complex, two phase processes. Namely, this approach allows one to escape from the model of this process usually described by a set of partial differential equations and enables one, using this method, to simulate evaporators dynamics in an extraordinarily simple way. In current literature this approach is sometimes called physical discretization. (author)

TOWARDS A MULTI-SCALE AGENT-BASED PROGRAMMING LANGUAGE METHODOLOGY.

Science.gov (United States)

Somogyi, Endre; Hagar, Amit; Glazier, James A

2016-12-01

Living tissues are dynamic, heterogeneous compositions of objects , including molecules, cells and extra-cellular materials, which interact via chemical, mechanical and electrical process and reorganize via transformation, birth, death and migration processes . Current programming language have difficulty describing the dynamics of tissues because: 1: Dynamic sets of objects participate simultaneously in multiple processes, 2: Processes may be either continuous or discrete, and their activity may be conditional, 3: Objects and processes form complex, heterogeneous relationships and structures, 4: Objects and processes may be hierarchically composed, 5: Processes may create, destroy and transform objects and processes. Some modeling languages support these concepts, but most cannot translate models into executable simulations. We present a new hybrid executable modeling language paradigm, the Continuous Concurrent Object Process Methodology ( CCOPM ) which naturally expresses tissue models, enabling users to visually create agent-based models of tissues, and also allows computer simulation of these models.

Integration and Differentiation as the Universal Scientific Categories and their Reflection in the Theory and Practice of Natural Science Education

Directory of Open Access Journals (Sweden)

V. A. Ignatova

2013-01-01

Full Text Available The post-industrial society gives way to the qualitatively new formation of education, integrated at its every level: integration with science and production; cooperation of different educational establishments; succession of educational levels; cross-disciplinary and inter-disciplinary expertise development; choice of methods, technologies and organizational forms of education and upbringing, etc. The integration and differentiation in their didactic unity reflect the complexity and contradiction of educational process, either of them dominating in certain socio-economic conditions of the given historic period. The retrospective analysis of the above correlation regarding the natural science disciplines demonstrates the lack of theoretical and methodological bases for integration, and its accidental unsystematic character in educational processes. The main conclusion of the study is the need for the complex competence model to combine the ideas of integration and differentiation providing both the wide outlook and professional training. For overcoming the predominance of differentiated education, the author suggests adapting the concepts of post-non-classical science, and selection and structuring of educational information with the reference to the semantic universals of systematic synergetic approach. The research findings can be used in pedagogic research methodology, educational process design and modeling, its content, technology and organization. 

Extrinsic factors promoting insulin producing cell-differentiation and insulin expression enhancement-hope for diabetics.

Science.gov (United States)

Dave, Shruti

2013-11-01

Diabetes mellitus (DM) is considered to be an autoimmune disorder leading to destruction of beta-cells resulting in to a loss of blood sugar control. Attempts using many pharmacological compositions including exogenous insulin have failed to show tight control of glycemia and associated manifestations. Stem cells are considered a potential tool for the supply of insulin-producing cells (IPC) generation in vitro. Stem cell differentiation in to pancreatic lineages requires influence of both intrinsic and extrinsic factors. Application of islet growth factors is considered to be potential for enhancement of beta-cell replication, function and survival. Use of certain extrinsic factors is known to facilitate expression of transcription factors known to be important for beta-cell differentiation and production of insulin enabling IPC generation. Hierarchies of secreted signals and transcription factors have been identified by studies from several laboratories that guide cell differentiation in to IPC. This knowledge provides insights for in vitro IPC differentiation from stem cells. Current advancement in medical knowledge promises an insulin independency for DM patients. The review sheds light on few specific extrinsic factors which facilitate differentiation of stem cells in to IPC in vitro have been discussed; which can be proven as a potential therapeutic option for treatment of DM and associated diseases.

Risk assessment methodology for extreme wind and missile effects on critical facilities

International Nuclear Information System (INIS)

Twisdale, L.A.; Dunn, W.L.

1985-01-01

The TORMIS methodology has been applied to a number of probabilistic risk assessments of critical facilities in the continental United States. These analyses have centered on the estimation of tornado missile impact and damage risks to individual targets as well as to groups of targets at specific plants. A number of advancements and generalizations in the approach have recently been made. These include: (1) generalization of windfield options to include straight winds (WINMIS) and hurricanes (HURMIS); (2) generalization of the scoring to enable analysis of Boolean system expressions for damage probabilities on compound series and parallel safety trains; (3) generalization of the failure criteria to include wind pressure as well as missile impact; (4) generalization of the plant modeling capability to enable more detailed treatment of targets partially or fully enclosed by vulnerable cladding and to allow tracking of missiles inside such enclosures; and (5) incorporation of windspeed criteria for structural failure and subsequent production of potential missiles. This paper will present some of the basic theory and key results of recent TORMIS, WINMIS, and HURMIS applications. The influence of uncertainties in the estimation process and the data needed for plant-specific risk assessments will also be discussed

Validation of differential gene expression algorithms: Application comparing fold-change estimation to hypothesis testing

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Bickel David R

2010-01-01

Full Text Available Abstract Background Sustained research on the problem of determining which genes are differentially expressed on the basis of microarray data has yielded a plethora of statistical algorithms, each justified by theory, simulation, or ad hoc validation and yet differing in practical results from equally justified algorithms. Recently, a concordance method that measures agreement among gene lists have been introduced to assess various aspects of differential gene expression detection. This method has the advantage of basing its assessment solely on the results of real data analyses, but as it requires examining gene lists of given sizes, it may be unstable. Results Two methodologies for assessing predictive error are described: a cross-validation method and a posterior predictive method. As a nonparametric method of estimating prediction error from observed expression levels, cross validation provides an empirical approach to assessing algorithms for detecting differential gene expression that is fully justified for large numbers of biological replicates. Because it leverages the knowledge that only a small portion of genes are differentially expressed, the posterior predictive method is expected to provide more reliable estimates of algorithm performance, allaying concerns about limited biological replication. In practice, the posterior predictive method can assess when its approximations are valid and when they are inaccurate. Under conditions in which its approximations are valid, it corroborates the results of cross validation. Both comparison methodologies are applicable to both single-channel and dual-channel microarrays. For the data sets considered, estimating prediction error by cross validation demonstrates that empirical Bayes methods based on hierarchical models tend to outperform algorithms based on selecting genes by their fold changes or by non-hierarchical model-selection criteria. (The latter two approaches have comparable

IoT-Based Information System for Healthcare Application: Design Methodology Approach

Directory of Open Access Journals (Sweden)

Damian Dziak

2017-06-01

Full Text Available Over the last few decades, life expectancy has increased significantly. However, elderly people who live on their own often need assistance due to mobility difficulties, symptoms of dementia or other health problems. In such cases, an autonomous supporting system may be helpful. This paper proposes the Internet of Things (IoT-based information system for indoor and outdoor use. Since the conducted survey of related works indicated a lack of methodological approaches to the design process, therefore a Design Methodology (DM, which approaches the design target from the perspective of the stakeholders, contracting authorities and potential users, is introduced. The implemented solution applies the three-axial accelerometer and magnetometer, Pedestrian Dead Reckoning (PDR, thresholding and the decision trees algorithm. Such an architecture enables the localization of a monitored person within four room-zones with accuracy; furthermore, it identifies falls and the activities of lying, standing, sitting and walking. Based on the identified activities, the system classifies current activities as normal, suspicious or dangerous, which is used to notify the healthcare staff about possible problems. The real-life scenarios validated the high robustness of the proposed solution. Moreover, the test results satisfied both stakeholders and future users and ensured further cooperation with the project.

Design Methodology for Magnetic Field-Based Soft Tri-Axis Tactile Sensors.

Science.gov (United States)

Wang, Hongbo; de Boer, Greg; Kow, Junwai; Alazmani, Ali; Ghajari, Mazdak; Hewson, Robert; Culmer, Peter

2016-08-24

Tactile sensors are essential if robots are to safely interact with the external world and to dexterously manipulate objects. Current tactile sensors have limitations restricting their use, notably being too fragile or having limited performance. Magnetic field-based soft tactile sensors offer a potential improvement, being durable, low cost, accurate and high bandwidth, but they are relatively undeveloped because of the complexities involved in design and calibration. This paper presents a general design methodology for magnetic field-based three-axis soft tactile sensors, enabling researchers to easily develop specific tactile sensors for a variety of applications. All aspects (design, fabrication, calibration and evaluation) of the development of tri-axis soft tactile sensors are presented and discussed. A moving least square approach is used to decouple and convert the magnetic field signal to force output to eliminate non-linearity and cross-talk effects. A case study of a tactile sensor prototype, MagOne, was developed. This achieved a resolution of 1.42 mN in normal force measurement (0.71 mN in shear force), good output repeatability and has a maximum hysteresis error of 3.4%. These results outperform comparable sensors reported previously, highlighting the efficacy of our methodology for sensor design.

Dosimetric methodology of the ICRP

International Nuclear Information System (INIS)

Eckerman, K.F.

1994-01-01

Establishment of guidance for the protection of workers and members of the public from radiation exposures necessitates estimation of the radiation dose to tissues of the body at risk. The dosimetric methodology formulated by the International Commission on Radiological Protection (ICRP) is intended to be responsive to this need. While developed for radiation protection, elements of the methodology are often applied in addressing other radiation issues; e.g., risk assessment. This chapter provides an overview of the methodology, discusses its recent extension to age-dependent considerations, and illustrates specific aspects of the methodology through a number of numerical examples

Arithmetic differential equations on $GL_n$, I: differential cocycles

OpenAIRE

Buium, Alexandru; Dupuy, Taylor

2013-01-01

The theory of differential equations has an arithmetic analogue in which derivatives are replaced by Fermat quotients. One can then ask what is the arithmetic analogue of a linear differential equation. The study of usual linear differential equations is the same as the study of the differential cocycle from $GL_n$ into its Lie algebra given by the logarithmic derivative. However we prove here that there are no such cocycles in the context of arithmetic differential equations. In sequels of t...

Design Methodology - Design Synthesis

DEFF Research Database (Denmark)

Andreasen, Mogens Myrup

2003-01-01

Design Methodology is part of our practice and our knowledge about designing, and it has been strongly supported by the establishing and work of a design research community. The aim of this article is to broaden the reader¿s view of designing and Design Methodology. This is done by sketching...... the development of Design Methodology through time and sketching some important approaches and methods. The development is mainly forced by changing industrial condition, by the growth of IT support for designing, but also by the growth of insight into designing created by design researchers.......ABSTRACT Design Methodology shall be seen as our understanding of how to design; it is an early (emerging late 60ies) and original articulation of teachable and learnable methodics. The insight is based upon two sources: the nature of the designed artefacts and the nature of human designing. Today...

Application of Six Sigma methodology to a diagnostic imaging process.

Science.gov (United States)

Taner, Mehmet Tolga; Sezen, Bulent; Atwat, Kamal M

2012-01-01

This paper aims to apply the Six Sigma methodology to improve workflow by eliminating the causes of failure in the medical imaging department of a private Turkish hospital. Implementation of the design, measure, analyse, improve and control (DMAIC) improvement cycle, workflow chart, fishbone diagrams and Pareto charts were employed, together with rigorous data collection in the department. The identification of root causes of repeat sessions and delays was followed by failure, mode and effect analysis, hazard analysis and decision tree analysis. The most frequent causes of failure were malfunction of the RIS/PACS system and improper positioning of patients. Subsequent to extensive training of professionals, the sigma level was increased from 3.5 to 4.2. The data were collected over only four months. Six Sigma's data measurement and process improvement methodology is the impetus for health care organisations to rethink their workflow and reduce malpractice. It involves measuring, recording and reporting data on a regular basis. This enables the administration to monitor workflow continuously. The improvements in the workflow under study, made by determining the failures and potential risks associated with radiologic care, will have a positive impact on society in terms of patient safety. Having eliminated repeat examinations, the risk of being exposed to more radiation was also minimised. This paper supports the need to apply Six Sigma and present an evaluation of the process in an imaging department.

Enabling distributed collaborative science

DEFF Research Database (Denmark)

Hudson, T.; Sonnenwald, Diane H.; Maglaughlin, K.

2000-01-01

To enable collaboration over distance, a collaborative environment that uses a specialized scientific instrument called a nanoManipulator is evaluated. The nanoManipulator incorporates visualization and force feedback technology to allow scientists to see, feel, and modify biological samples bein...

VEM: Virtual Enterprise Methodology

DEFF Research Database (Denmark)

Tølle, Martin; Vesterager, Johan

2003-01-01

This chapter presents a virtual enterprise methodology (VEM) that outlines activities to consider when setting up and managing virtual enterprises (VEs). As a methodology the VEM helps companies to ask the right questions when preparing for and setting up an enterprise network, which works...

The Methodological Dynamism of Grounded Theory

Directory of Open Access Journals (Sweden)

Nicholas Ralph

2015-11-01

Full Text Available Variations in grounded theory (GT interpretation are the subject of ongoing debate. Divergences of opinion, genres, approaches, methodologies, and methods exist, resulting in disagreement on what GT methodology is and how it comes to be. From the postpositivism of Glaser and Strauss, to the symbolic interactionist roots of Strauss and Corbin, through to the constructivism of Charmaz, the field of GT methodology is distinctive in the sense that those using it offer new ontological, epistemological, and methodological perspectives at specific moments in time. We explore the unusual dynamism attached to GT’s underpinnings. Our view is that through a process of symbolic interactionism, in which generations of researchers interact with their context, moments are formed and philosophical perspectives are interpreted in a manner congruent with GT’s essential methods. We call this methodological dynamism, a process characterized by contextual awareness and moment formation, contemporaneous translation, generational methodology, and methodological consumerism.

Laboratory tests of overpressure differential systems for smoke protection of lobbies

Science.gov (United States)

Szałański, Paweł; Misiński, Jacek

2017-11-01

Paper presents the methodology of laboratory tests for ventilation overpressure differential systems for smoke protection of lobbies. Research area consists of two spaces representing the lobby and the area under fire equipped with proper ventilation installation. This allows testing of overpressure differential systems for smoke protection of lobbies. Moreover, piece of laboratory tests results for two selected smoke protection systems for lobbies are presented. First one is standard system with constantly opened transfer-damper mounted between lobby and area under fire. Second one - system with so called "electronic transfer" based on two dampers (supplying air to a lobby and to unprotected area alternatively). Opening and closing both dampers is electronically controlled. Changes of pressure difference between lobby and fire affected area during closing and opening doors between those spaces is presented. Conclusions, concerning the possibility of meeting the time period criteria of pressure difference stabilization required by standards, are presented and discussed for both systems.

Keeping it pure – a pedagogical case study of teaching soft systems methodology in scenario and policy analysis

Directory of Open Access Journals (Sweden)

Ian Yeoman

2016-09-01

Full Text Available Purpose – Soft systems methodology (SSM is well documented in the academic and management literature. Over the last 40 years, the methodology has come to be adapted depending on the tool users’ skills and experience in order to fit the problem. The purpose of this paper is to demonstrate good teaching and learning practice from a pedagogical perspective. Design/methodology/approach – Dr Ian Yeoman of Victoria University of Wellington provides a personal reflection of how the methodology is used in the teaching and learning of TOUR301 Tourism Policy and Planning as a policy and scenario analysis method. Findings – The paper articulates the seven stages of SSM from problem situation unstructured, through to Rich Pictures, vision and guiding principles, policy solutions, comparisons, feasibility and implementation stages. The paper uses a series of teaching tasks to breakdown the complexity of the methodology thus guiding students and teachers in how to deploy the methodology in the classroom. Originality/value – The value of the paper demonstrates the reflective practice of SSM in action as an exemplar of good practice. The paper clearly articulates the stages of the methodology so students and teachers can adopt this approach in classroom environments following a scaffolding learning approach. The use of teaching tasks throughout the paper helps bring clarity and order thus enabling the teacher to effectively teach the subject and the students to learn. The most significant contribution of this paper is the articulation of good teaching practice in policy and scenario analysis which articulated through four learning lessons: facilitating a learning environment; the impact of visual thinking; political theory; the importance of incremental learning; and problem-based learning and international students.

Thallium 201 thyroid scan: differential diagnosis of benign and malignant nodules

International Nuclear Information System (INIS)

Oh, Jong Sub; Kim, Byong Geun; Park, Byung Ran; Kim, Se Jong; Ko, Kang Seok; Kim, Min Joong; Ji, Joo Yun

1995-01-01

To evaluate useful findings and diagnostic value of TI-201 thyroid scan in differentiating benign from malignant nodules. We studied 77 cold thyroid nodules proven histologically(27 malignant and 50 benign). Early (5-15 min) and delayed images(3-5 hours) were obtained after intravenous injection of thallium 201. In these nodules, we retrospectively analyzed the degree of TI-201 uptake in early and delayed images, histopathologic type, size, and presence or absence of cystic change in the sonograms of 22 malignant nodules. Useful finding for diagnosis of malignant nodules was strong uptake of TI-201 in early and delayed images(specificity: 98%, sensitivity: 63%, positive predictive value: 94.4%). Useful finding for benign nodules was no uptake of TI-201 in delayed image(specificity: 88.9%, sensitivity: 68%, positive predictive value: 91.9%). The accuracy of TI-201 thyroid scan in differentiating benign from malignant nodules was 66.2%. The nodules with strong TI-201 uptake in early image and low TI-201 uptake in delayed image were malignant in 29.4%. Cystic changes were found in 40% of malignant nodules with atypical TI-201 uptake. TI-201 thyroid scan showed high specificity in follicular neoplasm and adenomatous goiter in which differentiation of benignancy and malignancy is difficult with only cytologic examination. We consider that TI-201 thyroid scan is valuable in differentiating benign from malignant nodules and when combined with fine needle aspiration and ultrasound examination, it will enable more accurate differential diagnosis between benign and malignant thyroid nodules

Comparative study on software development methodologies

Directory of Open Access Journals (Sweden)

Mihai Liviu DESPA

2014-12-01

Full Text Available This paper focuses on the current state of knowledge in the field of software development methodologies. It aims to set the stage for the formalization of a software development methodology dedicated to innovation orientated IT projects. The paper starts by depicting specific characteristics in software development project management. Managing software development projects involves techniques and skills that are proprietary to the IT industry. Also the software development project manager handles challenges and risks that are predominantly encountered in business and research areas that involve state of the art technology. Conventional software development stages are defined and briefly described. Development stages are the building blocks of any software development methodology so it is important to properly research this aspect. Current software development methodologies are presented. Development stages are defined for every showcased methodology. For each methodology a graphic representation is illustrated in order to better individualize its structure. Software development methodologies are compared by highlighting strengths and weaknesses from the stakeholder's point of view. Conclusions are formulated and a research direction aimed at formalizing a software development methodology dedicated to innovation orientated IT projects is enunciated.

The Methodology of Magpies

Science.gov (United States)

Carter, Susan

2014-01-01

Arts/Humanities researchers frequently do not explain methodology overtly; instead, they "perform" it through their use of language, textual and historic cross-reference, and theory. Here, methodologies from literary studies are shown to add to Higher Education (HE) an exegetical and critically pluralist approach. This includes…

Social-ecological enabling conditions for payments for ecosystem services

OpenAIRE

Heidi R. Huber-Stearns; Drew E. Bennett; Stephen Posner; Ryan C. Richards; Jenn Hoyle. Fair; Stella J. M. Cousins; Chelsie L. Romulo

2017-01-01

The concept of "enabling conditions" centers on conditions that facilitate approaches to addressing social and ecological challenges. Although multiple fields have independently addressed the concept of enabling conditions, the literature lacks a shared understanding or integration of concepts. We propose a more synthesized understanding of enabling conditions beyond disciplinary boundaries by focusing on the enabling conditions that influence the implementation of a range of environmental p...

Nanomaterial-Enabled Wearable Sensors for Healthcare.

Science.gov (United States)

Yao, Shanshan; Swetha, Puchakayala; Zhu, Yong

2018-01-01

Highly sensitive wearable sensors that can be conformably attached to human skin or integrated with textiles to monitor the physiological parameters of human body or the surrounding environment have garnered tremendous interest. Owing to the large surface area and outstanding material properties, nanomaterials are promising building blocks for wearable sensors. Recent advances in the nanomaterial-enabled wearable sensors including temperature, electrophysiological, strain, tactile, electrochemical, and environmental sensors are presented in this review. Integration of multiple sensors for multimodal sensing and integration with other components into wearable systems are summarized. Representative applications of nanomaterial-enabled wearable sensors for healthcare, including continuous health monitoring, daily and sports activity tracking, and multifunctional electronic skin are highlighted. Finally, challenges, opportunities, and future perspectives in the field of nanomaterial-enabled wearable sensors are discussed. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Update of Part 61 Impacts Analysis Methodology. Methodology report. Volume 1

International Nuclear Information System (INIS)

Oztunali, O.I.; Roles, G.W.

1986-01-01

Under contract to the US Nuclear Regulatory Commission, the Envirosphere Company has expanded and updated the impacts analysis methodology used during the development of the 10 CFR Part 61 rule to allow improved consideration of the costs and impacts of treatment and disposal of low-level waste that is close to or exceeds Class C concentrations. The modifications described in this report principally include: (1) an update of the low-level radioactive waste source term, (2) consideration of additional alternative disposal technologies, (3) expansion of the methodology used to calculate disposal costs, (4) consideration of an additional exposure pathway involving direct human contact with disposed waste due to a hypothetical drilling scenario, and (5) use of updated health physics analysis procedures (ICRP-30). Volume 1 of this report describes the calculational algorithms of the updated analysis methodology

Update of Part 61 Impacts Analysis Methodology. Methodology report. Volume 1

Energy Technology Data Exchange (ETDEWEB)

Oztunali, O.I.; Roles, G.W.

1986-01-01

Under contract to the US Nuclear Regulatory Commission, the Envirosphere Company has expanded and updated the impacts analysis methodology used during the development of the 10 CFR Part 61 rule to allow improved consideration of the costs and impacts of treatment and disposal of low-level waste that is close to or exceeds Class C concentrations. The modifications described in this report principally include: (1) an update of the low-level radioactive waste source term, (2) consideration of additional alternative disposal technologies, (3) expansion of the methodology used to calculate disposal costs, (4) consideration of an additional exposure pathway involving direct human contact with disposed waste due to a hypothetical drilling scenario, and (5) use of updated health physics analysis procedures (ICRP-30). Volume 1 of this report describes the calculational algorithms of the updated analysis methodology.

On the security of SSL/TLS-enabled applications

Directory of Open Access Journals (Sweden)

Manik Lal Das

2014-01-01

Full Text Available SSL/TLS (Secure Socket Layer/Transport Layer Security-enabled web applications aim to provide public key certificate based authentication, secure session key establishment, and symmetric key based traffic confidentiality. A large number of electronic commerce applications, such as stock trading, banking, shopping, and gaming rely on the security strength of the SSL/TLS protocol. In recent times, a potential threat, known as main-in-the-middle (MITM attack, has been exploited by attackers of SSL/TLS-enabled web applications, particularly when naive users want to connect to an SSL/TLS-enabled web server. In this paper, we discuss about the MITM threat to SSL/TLS-enabled web applications. We review the existing space of solutions to counter the MITM attack on SSL/TLS-enabled applications, and then, we provide an effective solution which can resist the MITM attack on SSL/TLS-enabled applications. The proposed solution uses a soft-token based approach for user authentication on top of the SSL/TLS’s security features. We show that the proposed solution is secure, efficient and user friendly in comparison to other similar approaches.

Methodological aspect of research of the process of socialization in media-cultural space of information society

Directory of Open Access Journals (Sweden)

N. Y. Hirlina

2016-03-01

Full Text Available Integrated within the social and philosophical discourse interdisciplinary methodology, based on the classic philosophical methodology for the analysis of socio-cultural phenomena enables a holistic understanding of the studied phenomenon. From a methodological point of view it is important to determine the social and philosophical understanding of the impact medіa cultural space of personality in conditions of dynamically changing socio-cultural environment. important social and philosophical methodological guideline should be considered on a thesis constant presence in the media culture of human space as being due to the fact that man is a social being, and the information society without media culture as its attribute exists. Philosophical «core» study of the spiritual culture of youth is humanism in its broadest sense, that is, understanding of the studied phenomenon primarily as a multi-dimensional culturing of human values. Submission materialistic determinant factors medіa cultural spiritual space is only possible under the dominance of humanistic values. With all the variety to understanding the spiritual dimension of the relationship of the individual with the socio-cultural environment common dominant philosophical idea of guidelines is the recognition of the spiritual and cultural autonomy rights. Globalization and its associated civilization and processes are seen as foreign in relation to social rights, while the internal spiritual content is cultural processes. Anthropological oriented cultural space of socialization based on interpersonal cultural interaction that produces unique and distinctive personality.

Monitoring costs in the ICU: a search for a pertinent methodology.

Science.gov (United States)

Reis Miranda, D; Jegers, M

2012-10-01

Attempts to determine costs in the intensive care unit (ICU) were not successful until now, as they failed to detect differences of costs between patients. The methodology and/or the instruments used might be at the origin of this failure. Based on the results of the European ICUs studies and on the descriptions of the activities of care in the ICU, we gathered and analysed the relevant literature concerning the monitoring of costs in the ICU. The aim was to formulate a methodology, from an economic perspective, in which future research may be framed. A bottom-up microcosting methodology will enable to distinguish costs between patients. The resulting information will at the same time support the decision-making of top management and be ready to include in the financial system of the hospital. Nursing staff explains about 30% of the total costs. This relation remains constant irrespective of the annual nurse/patient ratio. In contrast with other scoring instruments, the nursing activities score (NAS) covers all nursing activities. (1) NAS is to be chosen for quantifying nursing activities; (2) an instrument for measuring the physician's activities is not yet available; (3) because the nursing activities have a large impact on total costs, the standardisation of the processes of care (following the system approach) will contribute to manage costs, making also reproducible the issue of quality of care; (4) the quantification of the nursing activities may be the required (proxy) input for the automated bottom-up monitoring of costs in the ICU. © 2012 The Authors. Acta Anaesthesiologica Scandinavica © 2012 The Acta Anaesthesiologica Scandinavica Foundation.

The role of typography in differentiating look-alike/sound-alike drug names.

Science.gov (United States)

Gabriele, Sandra

2006-01-01

Until recently, when errors occurred in the course of caring for patients, blame was assigned to the healthcare professionals closest to the incident rather than examining the larger system and the actions that led up to the event. Now, the medical profession is embracing expertise and methodologies used in other fields to improve its own systems in relation to patient safety issues. This exploratory study, part of a Master's of Design thesis project, was a response to the problem of errors that occur due to confusion between look-alike/sound-alike drug names (medication names that have orthographic and/or phonetic similarities). The study attempts to provide a visual means to help differentiate problematic names using formal typographic and graphic cues. The FDA's Name Differentiation Project recommendations and other typographic alternatives were considered to address issues of attention and cognition. Eleven acute care nurses participated in testing that consisted of word-recognition tasks and questions intended to elicit opinions regarding the visual treatment of look-alike/sound-alike names in the context of a label prototype. Though limited in sample size, testing provided insight into the kinds of typographic differentiation that might be effective in a high-risk situation.

Integrated Photonics Enabled by Slow Light

DEFF Research Database (Denmark)

Mørk, Jesper; Chen, Yuntian; Ek, Sara

2012-01-01

In this talk we will discuss the physics of slow light in semiconductor materials and in particular the possibilities offered for integrated photonics. This includes ultra-compact slow light enabled optical amplifiers, lasers and pulse sources.......In this talk we will discuss the physics of slow light in semiconductor materials and in particular the possibilities offered for integrated photonics. This includes ultra-compact slow light enabled optical amplifiers, lasers and pulse sources....

Introduction to LCA Methodology

DEFF Research Database (Denmark)

Hauschild, Michael Z.

2018-01-01

In order to offer the reader an overview of the LCA methodology in the preparation of the more detailed description of its different phases, a brief introduction is given to the methodological framework according to the ISO 14040 standard and the main elements of each of its phases. Emphasis...

Methodologies, languages and tools

International Nuclear Information System (INIS)

Amako, Katsuya

1994-01-01

This is a summary of the open-quotes Methodologies, Languages and Toolsclose quotes session in the CHEP'94 conference. All the contributions to methodologies and languages are relevant to the object-oriented approach. Other topics presented are related to various software tools in the down-sized computing environment

Data Centric Development Methodology

Science.gov (United States)

Khoury, Fadi E.

2012-01-01

Data centric applications, an important effort of software development in large organizations, have been mostly adopting a software methodology, such as a waterfall or Rational Unified Process, as the framework for its development. These methodologies could work on structural, procedural, or object oriented based applications, but fails to capture…

“Do the next thing”: an interview with Elizabeth Adams St. Pierre on post-qualitative methodology

Directory of Open Access Journals (Sweden)

Hanna Guttorm

2015-06-01

Full Text Available Professor Elizabeth Adams St. Pierre’s work focuses on critical and poststructural theories of language and the subject and what she has called post qualitative inquiry or post inquiry. She asks what might come after conventional humanist qualitative research methodology. She’s especially interested in the new empiricisms/new materialisms as well as new research practices enabled by the ontological turn. During St. Pierre's visit to the Finnish Educational Research Association (FERA Conference in Oulu in November 2014, we had the opportunity to talk with her about post qualitative research around some questions we had sent her beforehand. We then transcribed, edited, and translated the interview, and published it in the Finnish journal Kasvatus (Education in spring 2015. In this interview St. Pierre talks in the US context, where qualitative methodology is turning—or being pushed to turn—back to positivism with normalized and formalized practices, St. Pierre encourages researchers to constantly question the prevailing truths and the traditions they have learnt too well.

CT differentiation of poorly-differentiated gastric neuroendocrine tumours from well-differentiated neuroendocrine tumours and gastric adenocarcinomas

Energy Technology Data Exchange (ETDEWEB)

Kim, Seong Ho; Kim, Se Hyung; Shin, Cheong-il; Han, Joon Koo; Choi, Byung Ihn [Seoul National University Hospital, Department of Radiology, Jongno-gu, Seoul (Korea, Republic of); Seoul National University Hospital, Institute of Radiation Medicine, Jongno-gu, Seoul (Korea, Republic of); Kim, Min-A [Seoul National University Hospital, Department of Pathology, Jongno-gu, Seoul (Korea, Republic of)

2015-07-15

To evaluate the differential CT features of gastric poorly-differentiated neuroendocrine tumours (PD-NETs) from well-differentiated NETs (WD-NETs) and gastric adenocarcinomas (ADCs) and to suggest differential features of hepatic metastases from gastric NETs and ADCs. Our study population was comprised of 36 patients with gastric NETs (18 WD-NETs, 18 PD-NETs) and 38 patients with gastric ADCs who served as our control group. Multiple CT features were assessed to identify significant differential CT findings of PD-NETs from WD-NETs and ADCs. In addition, CT features of hepatic metastases including the metastasis-to-liver ratio were analyzed to differentiate metastatic NETs from ADCs. The presence of metastatic lymph nodes was the sole differentiator of PD-NETs from WD-NETs (P =.001, odds ratio = 56.67), while the presence of intact overlying mucosa with mucosal tenting was the sole significant CT feature differentiating PD-NETs from ADCs (P =.047, odds ratio = 15.3) For hepatic metastases, metastases from NETs were more hyper-attenuated than those from ADCs. The presence of metastatic LNs and intact overlying mucosa with mucosal tenting are useful CT discriminators of PD-NETs from WD-NETs and ADCs, respectively. In addition, a higher metastasis-to-liver ratio may help differentiate hepatic metastases of gastric NETs from those of gastric ADCs with high accuracy. (orig.)

Social-ecological enabling conditions for payments for ecosystem services

Directory of Open Access Journals (Sweden)

Heidi R. Huber-Stearns

2017-03-01

Full Text Available The concept of "enabling conditions" centers on conditions that facilitate approaches to addressing social and ecological challenges. Although multiple fields have independently addressed the concept of enabling conditions, the literature lacks a shared understanding or integration of concepts. We propose a more synthesized understanding of enabling conditions beyond disciplinary boundaries by focusing on the enabling conditions that influence the implementation of a range of environmental policies termed payments for ecosystem services (PES. Through an analysis of key literature from different disciplinary perspectives, we examined how researchers and practitioners refer to and identify enabling conditions within the context of PES. Through our synthesis, we identified 24 distinct enabling conditions organized within 4 broad themes: biophysical, economic, governance, and social-cultural conditions. We found that the literature coalesces around certain enabling conditions, such as strong ecosystem science and existing institutions, regardless of disciplinary background or journal audience. We also observed key differences in how authors perceive the direction of influence for property type, program objectives, and number of actors. Additionally, we noted an emphasis on the importance of the contextual nature of many enabling conditions that may cause certain conditions to have a disproportionate impact on successful implementation in some circumstances. Unraveling the relative importance of specific enabling conditions in diverse contexts remains a research frontier. Ultimately, no single disciplinary perspective is likely to provide all necessary insights for PES creation, and given the intertwined nature of enabling conditions, practitioners need to consider insights from multiple dimensions. Our work suggests opportunities to better connect diverse conversations through integration of concepts, a common vocabulary, and a synthetic framework.

'Rapid Learning health care in oncology' - an approach towards decision support systems enabling customised radiotherapy'.

Science.gov (United States)

Lambin, Philippe; Roelofs, Erik; Reymen, Bart; Velazquez, Emmanuel Rios; Buijsen, Jeroen; Zegers, Catharina M L; Carvalho, Sara; Leijenaar, Ralph T H; Nalbantov, Georgi; Oberije, Cary; Scott Marshall, M; Hoebers, Frank; Troost, Esther G C; van Stiphout, Ruud G P M; van Elmpt, Wouter; van der Weijden, Trudy; Boersma, Liesbeth; Valentini, Vincenzo; Dekker, Andre

2013-10-01

An overview of the Rapid Learning methodology, its results, and the potential impact on radiotherapy. Rapid Learning methodology is divided into four phases. In the data phase, diverse data are collected about past patients, treatments used, and outcomes. Innovative information technologies that support semantic interoperability enable distributed learning and data sharing without additional burden on health care professionals and without the need for data to leave the hospital. In the knowledge phase, prediction models are developed for new data and treatment outcomes by applying machine learning methods to data. In the application phase, this knowledge is applied in clinical practice via novel decision support systems or via extensions of existing models such as Tumour Control Probability models. In the evaluation phase, the predictability of treatment outcomes allows the new knowledge to be evaluated by comparing predicted and actual outcomes. Personalised or tailored cancer therapy ensures not only that patients receive an optimal treatment, but also that the right resources are being used for the right patients. Rapid Learning approaches combined with evidence based medicine are expected to improve the predictability of outcome and radiotherapy is the ideal field to study the value of Rapid Learning. The next step will be to include patient preferences in the decision making. Copyright © 2013 The Authors. Published by Elsevier Ireland Ltd.. All rights reserved.

A methodological approach for designing and sequencing product families in Reconfigurable Disassembly Systems

Directory of Open Access Journals (Sweden)

Ignacio Eguia

2011-10-01

Full Text Available Purpose: A Reconfigurable Disassembly System (RDS represents a new paradigm of automated disassembly system that uses reconfigurable manufacturing technology for fast adaptation to changes in the quantity and mix of products to disassemble. This paper deals with a methodology for designing and sequencing product families in RDS. Design/methodology/approach: The methodology is developed in a two-phase approach, where products are first grouped into families and then families are sequenced through the RDS, computing the required machines and modules configuration for each family. Products are grouped into families based on their common features using a Hierarchical Clustering Algorithm. The optimal sequence of the product families is calculated using a Mixed-Integer Linear Programming model minimizing reconfigurability and operational costs. Findings: This paper is focused to enable reconfigurable manufacturing technologies to attain some degree of adaptability during disassembly automation design using modular machine tools. Research limitations/implications: The MILP model proposed for the second phase is similar to the well-known Travelling Salesman Problem (TSP and therefore its complexity grows exponentially with the number of products to disassemble. In real-world problems, which a higher number of products, it may be advisable to solve the model approximately with heuristics. Practical implications: The importance of industrial recycling and remanufacturing is growing due to increasing environmental and economic pressures. Disassembly is an important part of remanufacturing systems for reuse and recycling purposes. Automatic disassembly techniques have a growing number of applications in the area of electronics, aerospace, construction and industrial equipment. In this paper, a design and scheduling approach is proposed to apply in this area. Originality/value: This paper presents a new concept called Reconfigurable Disassembly System

Disposal Criticality Analysis Methodology Topical Report

International Nuclear Information System (INIS)

Horton, D.G.

1998-01-01

The fundamental objective of this topical report is to present the planned risk-informed disposal criticality analysis methodology to the NRC to seek acceptance that the principles of the methodology and the planned approach to validating the methodology are sound. The design parameters and environmental assumptions within which the waste forms will reside are currently not fully established and will vary with the detailed waste package design, engineered barrier design, repository design, and repository layout. Therefore, it is not practical to present the full validation of the methodology in this report, though a limited validation over a parameter range potentially applicable to the repository is presented for approval. If the NRC accepts the methodology as described in this section, the methodology will be fully validated for repository design applications to which it will be applied in the License Application and its references. For certain fuel types (e.g., intact naval fuel), a ny processes, criteria, codes or methods different from the ones presented in this report will be described in separate addenda. These addenda will employ the principles of the methodology described in this report as a foundation. Departures from the specifics of the methodology presented in this report will be described in the addenda

Disposal Criticality Analysis Methodology Topical Report

International Nuclear Information System (INIS)

D.G. Horton

1998-01-01

The fundamental objective of this topical report is to present the planned risk-informed disposal criticality analysis methodology to the NRC to seek acceptance that the principles of the methodology and the planned approach to validating the methodology are sound. The design parameters and environmental assumptions within which the waste forms will reside are currently not fully established and will vary with the detailed waste package design, engineered barrier design, repository design, and repository layout. Therefore, it is not practical to present the full validation of the methodology in this report, though a limited validation over a parameter range potentially applicable to the repository is presented for approval. If the NRC accepts the methodology as described in this section, the methodology will be fully validated for repository design applications to which it will be applied in the License Application and its references. For certain fuel types (e.g., intact naval fuel), any processes, criteria, codes or methods different from the ones presented in this report will be described in separate addenda. These addenda will employ the principles of the methodology described in this report as a foundation. Departures from the specifics of the methodology presented in this report will be described in the addenda

Safety analysis methodology for OPR 1000

International Nuclear Information System (INIS)

Hwang-Yong, Jun

2005-01-01

Full text: Korea Electric Power Research Institute (KEPRI) has been developing inhouse safety analysis methodology based on the delicate codes available to KEPRI to overcome the problems arising from currently used vendor oriented methodologies. For the Loss of Coolant Accident (LOCA) analysis, the KREM (KEPRI Realistic Evaluation Methodology) has been developed based on the RELAP-5 code. The methodology was approved for the Westinghouse 3-loop plants by the Korean regulatory organization and the project to extent the methodology to the Optimized Power Reactor 1000 (OPR1000) has been ongoing since 2001. Also, for the Non-LOCA analysis, the KNAP (Korea Non-LOCA Analysis Package) has been developed using the UNICORN-TM code system. To demonstrate the feasibility of these codes systems and methodologies, some typical cases of the design basis accidents mentioned in the final safety analysis report (FSAR) were analyzed. (author)

Methodological Reflections on the Use of Asynchronous Online Focus Groups in Health Research

Directory of Open Access Journals (Sweden)

Sarah Williams PhD

2012-09-01

Full Text Available The Internet is increasingly used as a tool in qualitative research. In particular, asynchronous online focus groups are used when factors such as cost, time, or access to participants can make conducting face-to-face research difficult. In this article we consider key methodological issues involved in using asynchronous online focus groups to explore experiences of health and illness. The written nature of Internet communication, the lack of physical presence, and the asynchronous, longitudinal aspects enable participants who might not normally contribute to research studies to reflect on their personal stories before disclosing them to the researcher. Implications for study design, recruitment strategies, and ethics should be considered when deciding whether to use this method.

Frequency Modulation of Transcriptional Bursting Enables Sensitive and Rapid Gene Regulation.

Science.gov (United States)

Li, Congxin; Cesbron, François; Oehler, Michael; Brunner, Michael; Höfer, Thomas

2018-04-25

Gene regulation is a complex non-equilibrium process. Here, we show that quantitating the temporal regulation of key gene states (transcriptionally inactive, active, and refractory) provides a parsimonious framework for analyzing gene regulation. Our theory makes two non-intuitive predictions. First, for transcription factors (TFs) that regulate transcription burst frequency, as opposed to amplitude or duration, weak TF binding is sufficient to elicit strong transcriptional responses. Second, refractoriness of a gene after a transcription burst enables rapid responses to stimuli. We validate both predictions experimentally by exploiting the natural, optogenetic-like responsiveness of the Neurospora GATA-type TF White Collar Complex (WCC) to blue light. Further, we demonstrate that differential regulation of WCC target genes is caused by different gene activation rates, not different TF occupancy, and that these rates are tuned by both the core promoter and the distance between TF-binding site and core promoter. In total, our work demonstrates the relevance of a kinetic, non-equilibrium framework for understanding transcriptional regulation. Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.

Differential transimpedance amplifier circuit for correlated differential amplification

Science.gov (United States)

Gresham, Christopher A [Albuquerque, NM; Denton, M Bonner [Tucson, AZ; Sperline, Roger P [Tucson, AZ

2008-07-22

A differential transimpedance amplifier circuit for correlated differential amplification. The amplifier circuit increase electronic signal-to-noise ratios in charge detection circuits designed for the detection of very small quantities of electrical charge and/or very weak electromagnetic waves. A differential, integrating capacitive transimpedance amplifier integrated circuit comprising capacitor feedback loops performs time-correlated subtraction of noise.

Menopause and Methodological Doubt

Science.gov (United States)

Spence, Sheila

2005-01-01

Menopause and methodological doubt begins by making a tongue-in-cheek comparison between Descartes' methodological doubt and the self-doubt that can arise around menopause. A hermeneutic approach is taken in which Cartesian dualism and its implications for the way women are viewed in society are examined, both through the experiences of women…

A pertinent approach to solve nonlinear fuzzy integro-differential equations.

Science.gov (United States)

Narayanamoorthy, S; Sathiyapriya, S P

2016-01-01

Fuzzy integro-differential equations is one of the important parts of fuzzy analysis theory that holds theoretical as well as applicable values in analytical dynamics and so an appropriate computational algorithm to solve them is in essence. In this article, we use parametric forms of fuzzy numbers and suggest an applicable approach for solving nonlinear fuzzy integro-differential equations using homotopy perturbation method. A clear and detailed description of the proposed method is provided. Our main objective is to illustrate that the construction of appropriate convex homotopy in a proper way leads to highly accurate solutions with less computational work. The efficiency of the approximation technique is expressed via stability and convergence analysis so as to guarantee the efficiency and performance of the methodology. Numerical examples are demonstrated to verify the convergence and it reveals the validity of the presented numerical technique. Numerical results are tabulated and examined by comparing the obtained approximate solutions with the known exact solutions. Graphical representations of the exact and acquired approximate fuzzy solutions clarify the accuracy of the approach.

Hippo Signaling Influences HNF4A and FOXA2 Enhancer Switching during Hepatocyte Differentiation

Directory of Open Access Journals (Sweden)

Olivia Alder

2014-10-01

Full Text Available Summary: Cell fate acquisition is heavily influenced by direct interactions between master regulators and tissue-specific enhancers. However, it remains unclear how lineage-specifying transcription factors, which are often expressed in both progenitor and mature cell populations, influence cell differentiation. Using in vivo mouse liver development as a model, we identified thousands of enhancers that are bound by the master regulators HNF4A and FOXA2 in a differentiation-dependent manner, subject to chromatin remodeling, and associated with differentially expressed target genes. Enhancers exclusively occupied in the embryo were found to be responsive to developmentally regulated TEAD2 and coactivator YAP1. Our data suggest that Hippo signaling may affect hepatocyte differentiation by influencing HNF4A and FOXA2 interactions with temporal enhancers. In summary, transcription factor-enhancer interactions are not only tissue specific but also differentiation dependent, which is an important consideration for researchers studying cancer biology or mammalian development and/or using transformed cell lines. : It is unclear how key transcription factors are critical for both lineage specification during embryonic development and maintenance of a differentiated, adult phenotype. By profiling the enhancer occupancy of the key transcription factors HNF4A and FOXA2 during mouse liver development, Alder et al. have found that YAP1 can influence enhancer interactions and target gene expression levels. Enhancer switching enables HNF4A and FOXA2 to fulfill distinct roles during organ development.

Smart Grid Enabled EVSE

Energy Technology Data Exchange (ETDEWEB)

None, None

2015-01-12

The combined team of GE Global Research, Federal Express, National Renewable Energy Laboratory, and Consolidated Edison has successfully achieved the established goals contained within the Department of Energy’s Smart Grid Capable Electric Vehicle Supply Equipment funding opportunity. The final program product, shown charging two vehicles in Figure 1, reduces by nearly 50% the total installed system cost of the electric vehicle supply equipment (EVSE) as well as enabling a host of new Smart Grid enabled features. These include bi-directional communications, load control, utility message exchange and transaction management information. Using the new charging system, Utilities or energy service providers will now be able to monitor transportation related electrical loads on their distribution networks, send load control commands or preferences to individual systems, and then see measured responses. Installation owners will be able to authorize usage of the stations, monitor operations, and optimally control their electricity consumption. These features and cost reductions have been developed through a total system design solution.

A Novel Methodology for Improving Plant Pest Surveillance in Vineyards and Crops Using UAV-Based Hyperspectral and Spatial Data.

Science.gov (United States)

Vanegas, Fernando; Bratanov, Dmitry; Powell, Kevin; Weiss, John; Gonzalez, Felipe

2018-01-17

Recent advances in remote sensed imagery and geospatial image processing using unmanned aerial vehicles (UAVs) have enabled the rapid and ongoing development of monitoring tools for crop management and the detection/surveillance of insect pests. This paper describes a (UAV) remote sensing-based methodology to increase the efficiency of existing surveillance practices (human inspectors and insect traps) for detecting pest infestations (e.g., grape phylloxera in vineyards). The methodology uses a UAV integrated with advanced digital hyperspectral, multispectral, and RGB sensors. We implemented the methodology for the development of a predictive model for phylloxera detection. In this method, we explore the combination of airborne RGB, multispectral, and hyperspectral imagery with ground-based data at two separate time periods and under different levels of phylloxera infestation. We describe the technology used-the sensors, the UAV, and the flight operations-the processing workflow of the datasets from each imagery type, and the methods for combining multiple airborne with ground-based datasets. Finally, we present relevant results of correlation between the different processed datasets. The objective of this research is to develop a novel methodology for collecting, processing, analising and integrating multispectral, hyperspectral, ground and spatial data to remote sense different variables in different applications, such as, in this case, plant pest surveillance. The development of such methodology would provide researchers, agronomists, and UAV practitioners reliable data collection protocols and methods to achieve faster processing techniques and integrate multiple sources of data in diverse remote sensing applications.

A Novel Methodology for Improving Plant Pest Surveillance in Vineyards and Crops Using UAV-Based Hyperspectral and Spatial Data

Science.gov (United States)

Vanegas, Fernando; Weiss, John; Gonzalez, Felipe

2018-01-01

Recent advances in remote sensed imagery and geospatial image processing using unmanned aerial vehicles (UAVs) have enabled the rapid and ongoing development of monitoring tools for crop management and the detection/surveillance of insect pests. This paper describes a (UAV) remote sensing-based methodology to increase the efficiency of existing surveillance practices (human inspectors and insect traps) for detecting pest infestations (e.g., grape phylloxera in vineyards). The methodology uses a UAV integrated with advanced digital hyperspectral, multispectral, and RGB sensors. We implemented the methodology for the development of a predictive model for phylloxera detection. In this method, we explore the combination of airborne RGB, multispectral, and hyperspectral imagery with ground-based data at two separate time periods and under different levels of phylloxera infestation. We describe the technology used—the sensors, the UAV, and the flight operations—the processing workflow of the datasets from each imagery type, and the methods for combining multiple airborne with ground-based datasets. Finally, we present relevant results of correlation between the different processed datasets. The objective of this research is to develop a novel methodology for collecting, processing, analysing and integrating multispectral, hyperspectral, ground and spatial data to remote sense different variables in different applications, such as, in this case, plant pest surveillance. The development of such methodology would provide researchers, agronomists, and UAV practitioners reliable data collection protocols and methods to achieve faster processing techniques and integrate multiple sources of data in diverse remote sensing applications. PMID:29342101

Cognitive Spectrum Sharing: An Enabling Wireless Communication Technology for a Wide Use of Smart Systems

Directory of Open Access Journals (Sweden)

Romano Fantacci

2016-05-01

Full Text Available A smart city is an environment where a pervasive, multi-service network is employed to provide citizens improved living conditions as well as better public safety and security. Advanced communication technologies are essential to achieve this goal. In particular, an efficient and reliable communication network plays a crucial role in providing continue, ubiquitous, and reliable interconnections among users, smart devices, and applications. As a consequence, wireless networking appears as the principal enabling communication technology despite the necessity to face severe challenges to satisfy the needs arising from a smart environment, such as explosive data volume, heterogeneous data traffic, and support of quality of service constraints. An interesting approach for meeting the growing data demand due to smart city applications is to adopt suitable methodologies to improve the usage of all potential spectrum resources. Towards this goal, a very promising solution is represented by the Cognitive Radio technology that enables context-aware capability in order to pursue an efficient use of the available communication resources according to the surrounding environment conditions. In this paper we provide a review of the characteristics, challenges, and solutions of a smart city communication architecture, based on the Cognitive Radio technology, by focusing on two new network paradigms—namely, Heterogeneous Network and Machines-to-Machines communications—that are of special interest to efficiently support smart city applications and services.

[Hepatic cell transplantation. Technical and methodological aspects].

Science.gov (United States)

Pareja, Eugenia; Martínez, Amparo; Cortés, Miriam; Bonora, Ana; Moya, Angel; Sanjuán, Fernando; Gómez-Lechón, M José; Mir, José

2010-03-01

Hepatic cell transplantation consists of grafting already differentiated cells such as hepatocytes. Human hepatocytes are viable and functionally active. Liver cell transplantation is carried out by means of a 3-step method: isolation of hepatocytes from donor liver rejected for orthotopic transplantation, preparing a cell suspension for infusion and, finally, hepatocytes are implanted into the recipient. There are established protocols for the isolation of human hepatocytes from unused segments of donor livers, based on collagenase digestion of cannulated liver tissue at 37 degrees C. The hepatocytes can be used fresh or cryopreserved. Cryopreservation of isolated human hepatocytes would then be available for planned use. In cell transplant, the important aspects are: infusion route, number of cells, number of infusions and viability of the cells. The cells are infused into the patient through a catheter inserted via portal vein or splenic artery. Liver cell transplantation allows liver tissue to be used that would, otherwise, be discarded, enabling multiple patients to be treated with hepatocytes from a single tissue donor. Copyright 2009 AEC. Published by Elsevier Espana. All rights reserved.

Information technology security system engineering methodology

Science.gov (United States)

Childs, D.

2003-01-01

A methodology is described for system engineering security into large information technology systems under development. The methodology is an integration of a risk management process and a generic system development life cycle process. The methodology is to be used by Security System Engineers to effectively engineer and integrate information technology security into a target system as it progresses through the development life cycle. The methodology can also be used to re-engineer security into a legacy system.

Assessing the performance of a differential evolution algorithm in structural damage detection by varying the objective function

OpenAIRE

Villalba-Morales, Jesús Daniel; Laier, José Elias

2014-01-01

Structural damage detection has become an important research topic in certain segments of the engineering community. These methodologies occasionally formulate an optimization problem by defining an objective function based on dynamic parameters, with metaheuristics used to find the solution. In this study, damage localization and quantification is performed by an Adaptive Differential Evolution algorithm, which solves the associated optimization problem. Furthermore, this paper looks at the ...

A stochastic differential equation analysis of cerebrospinal fluid dynamics

Directory of Open Access Journals (Sweden)

Raman Kalyan

2011-01-01

Full Text Available Abstract Background Clinical measurements of intracranial pressure (ICP over time show fluctuations around the deterministic time path predicted by a classic mathematical model in hydrocephalus research. Thus an important issue in mathematical research on hydrocephalus remains unaddressed--modeling the effect of noise on CSF dynamics. Our objective is to mathematically model the noise in the data. Methods The classic model relating the temporal evolution of ICP in pressure-volume studies to infusions is a nonlinear differential equation based on natural physical analogies between CSF dynamics and an electrical circuit. Brownian motion was incorporated into the differential equation describing CSF dynamics to obtain a nonlinear stochastic differential equation (SDE that accommodates the fluctuations in ICP. Results The SDE is explicitly solved and the dynamic probabilities of exceeding critical levels of ICP under different clinical conditions are computed. A key finding is that the probabilities display strong threshold effects with respect to noise. Above the noise threshold, the probabilities are significantly influenced by the resistance to CSF outflow and the intensity of the noise. Conclusions Fluctuations in the CSF formation rate increase fluctuations in the ICP and they should be minimized to lower the patient's risk. The nonlinear SDE provides a scientific methodology for dynamic risk management of patients. The dynamic output of the SDE matches the noisy ICP data generated by the actual intracranial dynamics of patients better than the classic model used in prior research.

People avoid situations that enable them to deceive others

NARCIS (Netherlands)

Shalvi, S.; Handgraaf, M.J.J.; de Dreu, C.K.W.

2011-01-01

Information advantage enables people to benefit themselves by deceiving their counterparts. Using a modified ultimatum bargaining game with an exit option, we find that people are more likely to avoid settings enabling them to privately deceive their counterparts than settings which do not enable

Physical parameter determinations of young Ms. Taking advantage of the Virtual Observatory to compare methodologies

Science.gov (United States)

Bayo, A.; Rodrigo, C.; Barrado, D.; Allard, F.

One of the very first steps astronomers working in stellar physics perform to advance in their studies, is to determine the most common/relevant physical parameters of the objects of study (effective temperature, bolometric luminosity, surface gravity, etc.). Different methodologies exist depending on the nature of the data, intrinsic properties of the objects, etc. One common approach is to compare the observational data with theoretical models passed through some simulator that will leave in the synthetic data the same imprint than the observational data carries, and see what set of parameters reproduce the observations best. Even in this case, depending on the kind of data the astronomer has, the methodology changes slightly. After parameters are published, the community tend to quote, praise and criticize them, sometimes paying little attention on whether the possible discrepancies come from the theoretical models, the data themselves or just the methodology used in the analysis. In this work we perform the simple, yet interesting, exercise of comparing the effective temperatures obtained via SED and more detailed spectral fittings (to the same grid of models), of a sample of well known and characterized young M-type objects members to different star forming regions and show how differences in temperature of up to 350 K can be expected just from the difference in methodology/data used. On the other hand we show how these differences are smaller for colder objects even when the complexity of the fit increases like for example introducing differential extinction. To perform this exercise we benefit greatly from the framework offered by the Virtual Observaotry.

In search of epigenetic marks in testes and sperm cells of differentially fed boars.

Directory of Open Access Journals (Sweden)

Rémy Bruggmann

Full Text Available In search of transmittable epigenetic marks we investigated gene expression in testes and sperm cells of differentially fed F0 boars from a three generation pig feeding experiment that showed phenotypic differences in the F2 generation. RNA samples from 8 testes of boars that received either a diet enriched in methylating micronutrients or a control diet were analyzed by microarray analysis. We found moderate differential expression between testes of differentially fed boars with a high FDR of 0.82 indicating that most of the differentially expressed genes were false positives. Nevertheless, we performed a pathway analysis and found disparate pathway maps of development_A2B receptor: action via G-protein alpha s, cell adhesion_Tight junctions and cell adhesion_Endothelial cell contacts by junctional mechanisms which show inconclusive relation to epigenetic inheritance. Four RNA samples from sperm cells of these differentially fed boars were analyzed by RNA-Seq methodology. We found no differential gene expression in sperm cells of the two groups (adjusted P-value>0.05. Nevertheless, we also explored gene expression in sperm by a pathway analysis showing that genes were enriched for the pathway maps of bacterial infections in cystic fibrosis (CF airways, glycolysis and gluconeogenesis p.3 and cell cycle_Initiation of mitosis. Again, these pathway maps are miscellaneous without an obvious relationship to epigenetic inheritance. It is concluded that the methylating micronutrients moderately if at all affects RNA expression in testes of differentially fed boars. Furthermore, gene expression in sperm cells is not significantly affected by extensive supplementation of methylating micronutrients and thus RNA molecules could not be established as the epigenetic mark in this feeding experiment.

Determination of tenogenic differentiation in human mesenchymal stem cells by terahertz waves for measurement of the optical property of cellular suspensions

International Nuclear Information System (INIS)

Morita, Yasuyuki; Azuchi, Kosuke; Ju, Yang; Suzuki, Satoshi; Xu, Baiyao; Yamamoto, Shuhei

2014-01-01

Technology for identifying stem cell-to-tenocyte differentiation that is non-contact and non-destructive in vitro is essential in tissue engineering. It has been found that expression of various RNA and proteins produced by differentiated cells is elevated when human bone marrow mesenchymal stem cells (hBMSCs) differentiate into tenocytes. Also, such biomolecules have absorption bands in the terahertz range. Thus, we attempted to evaluate whether terahertz waves could be used to distinguish hBMSC-to-tenocyte differentiation. Terahertz time-domain spectroscopy (THz-TDS) using femtosecond laser pulses was used for terahertz measurements. HBMSCs differentiated into tenocytes with mechanical stimulation: 10% cyclical uniaxial stretching at 1 Hz for 24 or 48 h. Cellular suspensions before and after differentiation were measured with terahertz waves. Complex refractive index, consisting of a refractive index (real) and an extinction coefficient (imaginary) obtained from the transmitted terahertz signals, was evaluated before and after differentiation at 1.0 THz. As a result, the THz-TDS system enabled discrimination of hBMSC-to-tenocyte differentiation due to the marked contrast in optical parameter before and after differentiation. This is the first report of the potential of a THz-TDS system for the detection of tenogenic differentiation using a non-contact and non-destructive in vitro technique. (paper)

Determination of tenogenic differentiation in human mesenchymal stem cells by terahertz waves for measurement of the optical property of cellular suspensions

Science.gov (United States)

Morita, Yasuyuki; Azuchi, Kosuke; Ju, Yang; Suzuki, Satoshi; Xu, Baiyao; Yamamoto, Shuhei

2014-06-01

Technology for identifying stem cell-to-tenocyte differentiation that is non-contact and non-destructive in vitro is essential in tissue engineering. It has been found that expression of various RNA and proteins produced by differentiated cells is elevated when human bone marrow mesenchymal stem cells (hBMSCs) differentiate into tenocytes. Also, such biomolecules have absorption bands in the terahertz range. Thus, we attempted to evaluate whether terahertz waves could be used to distinguish hBMSC-to-tenocyte differentiation. Terahertz time-domain spectroscopy (THz-TDS) using femtosecond laser pulses was used for terahertz measurements. HBMSCs differentiated into tenocytes with mechanical stimulation: 10% cyclical uniaxial stretching at 1 Hz for 24 or 48 h. Cellular suspensions before and after differentiation were measured with terahertz waves. Complex refractive index, consisting of a refractive index (real) and an extinction coefficient (imaginary) obtained from the transmitted terahertz signals, was evaluated before and after differentiation at 1.0 THz. As a result, the THz-TDS system enabled discrimination of hBMSC-to-tenocyte differentiation due to the marked contrast in optical parameter before and after differentiation. This is the first report of the potential of a THz-TDS system for the detection of tenogenic differentiation using a non-contact and non-destructive in vitro technique.

The effect of differentiated margin on futures market investors' behavior and structure An experimental research

Institute of Scientific and Technical Information of China (English)

Jin-hui Luo; Di-fang Wan; Yang Yang; Guang Yang

2011-01-01

Purpose-The purpose of this paper is to empirically analyze the role of differentiated margin system in leading investors' investing behavior and then optimize investor structure in futures markets.Design/methodology/approach-Using economic experimental research method,this paper designs and conducts a futures market experiment according to experimental research's basic norms,thus acquiring needed and credible empirical data.Findings-By analyzing the experimental data,it is found that compared with situations in futures markets that implement uniform margin system,investors' (especially speculators') futures open position and the ratio of their open position and futures turnover are both significantly higher,in futures markets that implement differentiated margin system.On the other hand,differentiated margin system has no effects on hedgers' futures turnover,but significantly reduces speculators' futures turnover.Research limitations/implications-The findings suggest that compared with uniform margin system,differentiated margin system is beneficial to effectively restrict both speculators' and hedgers' speculating behavior and lead hedgers' market participation.Practical implications-In order to resolve the problem of unreasonable investor structure in China's futures market,i.e.lack of hedgers and over-speculating China's futures market's regulators should reform the margin system and adopt differentiated margin system to lead investors' rational behavior and optimize investor structure.Originality/value-This paper empirically analyzes and verifies,for the first time,the roles of differentiated margin system in affecting investors' investing behavior.The futures market experiment designed and used in this study is a pioneering and exploratory experiment.

Methodological practicalities in analytical generalization