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Sample records for metapneumovirus nipah west

  1. Human metapneumovirus infections in children.

    Science.gov (United States)

    Heikkinen, Terho; Osterback, Riikka; Peltola, Ville; Jartti, Tuomas; Vainionpää, Raija

    2008-01-01

    Human metapneumovirus (hMPV) is an important cause of lower respiratory tract infections in hospitalized children, but the age-related incidence and effect of hMPV in unselected children in the community have not been evaluated. We studied a cohort of 1,338 children <13 years of age throughout 1 respiratory season in Finland during 2000-2001. We examined children and obtained a nasal swab for viral detection at any sign of respiratory infection. hMPV was detected in 47 (3.5%) of the 1,338 children. The age-related incidence of hMPV infection was highest (7.6%) in children <2 years of age, in whom hMPV accounted for 1.7% of all infections during the season. During the epidemic peak, hMPV caused 7.1% of all respiratory infections in the cohort. Acute otitis media developed in 61% of hMPV-infected children <3 years of age. Our findings demonstrate that the effect of hMPV in the community is greatest in children <2 years of age.

  2. Cluster of Nipah virus infection, Kushtia District, Bangladesh, 2007.

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    Nusrat Homaira

    Full Text Available OBJECTIVE: In March 2007, we investigated a cluster of Nipah encephalitis to identify risk factors for Nipah infection in Bangladesh. METHODS: We defined confirmed Nipah cases by the presence of IgM and IgG antibodies against Nipah virus in serum. Case-patients, who resided in the same village during the outbreak period but died before serum could be collected, were classified as probable cases. RESULTS: We identified three confirmed and five probable Nipah cases. There was a single index case. Five of the secondary cases came in close physical contact to the index case when she was ill. Case-patients were more likely to have physical contact with the index case (71% cases versus 0% controls, p = <0.001. The index case, on her third day of illness, and all the subsequent cases attended the same religious gathering. For three probable cases including the index case, we could not identify any known risk factors for Nipah infection such as physical contact with Nipah case-patients, consumption of raw date palm juice, or contact with sick animals or fruit bats. CONCLUSION: Though person-to-person transmission remains an important mode of transmission for Nipah infection, we could not confirm the source of infection for three of the probable Nipah case-patients. Continued surveillance and outbreak investigations will help better understand the transmission of Nipah virus and develop preventive strategies.

  3. Clinical presentation of nipah virus infection in Bangladesh.

    Science.gov (United States)

    Hossain, M Jahangir; Gurley, Emily S; Montgomery, Joel M; Bell, Michael; Carroll, Darin S; Hsu, Vincent P; Formenty, P; Croisier, A; Bertherat, E; Faiz, M A; Azad, Abul Kalam; Islam, Rafiqul; Molla, M Abdur Rahim; Ksiazek, Thomas G; Rota, Paul A; Comer, James A; Rollin, Pierre E; Luby, Stephen P; Breiman, Robert F

    2008-04-01

    In Bangladesh, 4 outbreaks of Nipah virus infection were identified during the period 2001-2004. We characterized the clinical features of Nipah virus-infected individuals affected by these outbreaks. We classified patients as having confirmed cases of Nipah virus infection if they had antibodies reactive with Nipah virus antigen. Patients were considered to have probable cases of Nipah virus infection if they had symptoms consistent with Nipah virus infection during the same time and in the same community as patients with confirmed cases. We identified 92 patients with Nipah virus infection, 67 (73%) of whom died. Although all age groups were affected, 2 outbreaks principally affected young persons (median age, 12 years); 62% of the affected persons were male. Fever, altered mental status, headache, cough, respiratory difficulty, vomiting, and convulsions were the most common signs and symptoms; clinical and radiographic features of acute respiratory distress syndrome of Nipah illness were identified during the fourth outbreak. Among those who died, death occurred a median of 6 days (range, 2-36 days) after the onset of illness. Patients who died were more likely than survivors to have a temperature >37.8 degrees C, altered mental status, difficulty breathing, and abnormal plantar reflexes. Among patients with Nipah virus infection who had well-defined exposure to another patient infected with Nipah virus, the median incubation period was 9 days (range, 6-11 days). Nipah virus infection produced rapidly progressive severe illness affecting the central nervous and respiratory systems. Clinical characteristics of Nipah virus infection in Bangladesh, including a severe respiratory component, appear distinct from clinical characteristics reported during earlier outbreaks in other countries.

  4. Quantitative estimation of Nipah virus replication kinetics in vitro

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    Hassan Sharifah

    2006-06-01

    Full Text Available Abstract Background Nipah virus is a zoonotic virus isolated from an outbreak in Malaysia in 1998. The virus causes infections in humans, pigs, and several other domestic animals. It has also been isolated from fruit bats. The pathogenesis of Nipah virus infection is still not well described. In the present study, Nipah virus replication kinetics were estimated from infection of African green monkey kidney cells (Vero using the one-step SYBR® Green I-based quantitative real-time reverse transcriptase-polymerase chain reaction (qRT-PCR assay. Results The qRT-PCR had a dynamic range of at least seven orders of magnitude and can detect Nipah virus from as low as one PFU/μL. Following initiation of infection, it was estimated that Nipah virus RNA doubles at every ~40 minutes and attained peak intracellular virus RNA level of ~8.4 log PFU/μL at about 32 hours post-infection (PI. Significant extracellular Nipah virus RNA release occurred only after 8 hours PI and the level peaked at ~7.9 log PFU/μL at 64 hours PI. The estimated rate of Nipah virus RNA released into the cell culture medium was ~0.07 log PFU/μL per hour and less than 10% of the released Nipah virus RNA was infectious. Conclusion The SYBR® Green I-based qRT-PCR assay enabled quantitative assessment of Nipah virus RNA synthesis in Vero cells. A low rate of Nipah virus extracellular RNA release and low infectious virus yield together with extensive syncytial formation during the infection support a cell-to-cell spread mechanism for Nipah virus infection.

  5. Recombinant Measles Virus Vaccine Expressing the Nipah Virus Glycoprotein Protects against Lethal Nipah Virus Challenge

    OpenAIRE

    Misako Yoneda; Marie-Claude Georges-Courbot; Fusako Ikeda; Miho Ishii; Noriyo Nagata; Frederic Jacquot; Hervé Raoul; Hiroki Sato; Chieko Kai

    2013-01-01

    Nipah virus (NiV) is a member of the genus Henipavirus, which emerged in Malaysia in 1998. In pigs, infection resulted in a predominantly non-lethal respiratory disease; however, infection in humans resulted in over 100 deaths. Nipah virus has continued to re-emerge in Bangladesh and India, and person-to-person transmission appeared in the outbreak. Although a number of NiV vaccine studies have been reported, there are currently no vaccines or treatments licensed for human use. In this study,...

  6. Human metapneumovirus: a new respiratory pathogen

    Indian Academy of Sciences (India)

    S Broor; P Bharaj; H S Chahar

    2008-11-01

    Human metapneumovirus is a recently recognized pathogen of acute respiratory tract infection (ARI) in children as well as elderly and immunocompromised adults. The virus belongs to the family Paramyxoviridae, sub family Pneumovirinae and genus Metapneumovirus. Through genetic analysis it has been characterized into two groups A and B which are further divided into four sub-lineages. The virus is difficult to grow in tissue culture and hence reverse transcriptase-polymerase chain reaction (RT-PCR) for N and L gene is the method of choice for diagnosis. The virus has been seen in all countries with seasonal distribution in winter months for temperate and spring/summer for tropical countries. F gene is the most conserved among different lineages and efforts are underway to design recombination vaccine using F gene.

  7. Avian Metapneumovirus Molecular Biology and Development of Genetically Engineered Vaccines

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    Avian metapneumovirus (aMPV) is an economically important pathogen of turkeys with a worldwide distribution. aMPV is a member of the genus Metapneumovirus within the subfamily Pneumovirinae of the family Paramyxoviridae. The genome of aMPV is a non-segmented, single-stranded, negative-sense RNA of 1...

  8. Human Metapneumovirus Antagonism of Innate Immune Responses

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    Xiaoyong Bao

    2012-12-01

    Full Text Available  Human metapneumovirus (hMPV is a recently identified RNA virus belonging to the Paramyxoviridae family, which includes several major human and animal pathogens. Epidemiological studies indicate that hMPV is a significant human respiratory pathogen with worldwide distribution. It is associated with respiratory illnesses in children, adults, and immunocompromised patients, ranging from upper respiratory tract infections to severe bronchiolitis and pneumonia. Interferon (IFN represents a major line of defense against virus infection, and in response, viruses have evolved countermeasures to inhibit IFN production as well as IFN signaling. Although the strategies of IFN evasion are similar, the specific mechanisms by which paramyxoviruses inhibit IFN responses are quite diverse. In this review, we will present an overview of the strategies that hMPV uses to subvert cellular signaling in airway epithelial cells, the major target of infection, as well as in primary immune cells.

  9. The distribution of henipaviruses in Southeast Asia and Australasia: is Wallace's line a barrier to Nipah virus?

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    Andrew C Breed

    Full Text Available Nipah virus (NiV (Genus Henipavirus is a recently emerged zoonotic virus that causes severe disease in humans and has been found in bats of the genus Pteropus. Whilst NiV has not been detected in Australia, evidence for NiV-infection has been found in pteropid bats in some of Australia's closest neighbours. The aim of this study was to determine the occurrence of henipaviruses in fruit bat (Family Pteropodidae populations to the north of Australia. In particular we tested the hypothesis that Nipah virus is restricted to west of Wallace's Line. Fruit bats from Australia, Papua New Guinea, East Timor and Indonesia were tested for the presence of antibodies to Hendra virus (HeV and Nipah virus, and tested for the presence of HeV, NiV or henipavirus RNA by PCR. Evidence was found for the presence of Nipah virus in both Pteropus vampyrus and Rousettus amplexicaudatus populations from East Timor. Serology and PCR also suggested the presence of a henipavirus that was neither HeV nor NiV in Pteropus alecto and Acerodon celebensis. The results demonstrate the presence of NiV in the fruit bat populations on the eastern side of Wallace's Line and within 500 km of Australia. They indicate the presence of non-NiV, non-HeV henipaviruses in fruit bat populations of Sulawesi and Sumba and possibly in Papua New Guinea. It appears that NiV is present where P. vampyrus occurs, such as in the fruit bat populations of Timor, but where this bat species is absent other henipaviruses may be present, as on Sulawesi and Sumba. Evidence was obtained for the presence henipaviruses in the non-Pteropid species R. amplexicaudatus and in A. celebensis. The findings of this work fill some gaps in knowledge in geographical and species distribution of henipaviruses in Australasia which will contribute to planning of risk management and surveillance activities.

  10. Mission critical: mobilization of essential animal models for Ebola, Nipah, and Machupo virus infections.

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    Zumbrun, E E

    2015-01-01

    The reports for Ebola virus Zaire (EBOV), Nipah virus, and Machupo virus (MACV) pathogenesis, in this issue of Veterinary Pathology, are timely considering recent events, both nationally and internationally. EBOV, Nipah virus, and MACV cause highly lethal infections for which no Food and Drug Administration (FDA) licensed vaccines or therapies exist. Not only are there concerns that these agents could be used by those with malicious intent, but shifts in ecological distribution of viral reservoirs due to climate change or globalization could lead to more frequent infections within remote regions than previously seen as well as outbreaks in more populous areas. The current EBOV epidemic shows no sign of abating across 3 West African nations (as of October 2014), including densely populated areas, far outpacing infection rates of previous outbreaks. A limited number of cases have also arisen in the United States and Europe. With few treatment options for these deadly viruses, development of animal models reflective of human disease is paramount to combat these diseases. As an example of this potential, a new treatment compound, ZMapp, that had demonstrated efficacy against EBOV infection in nonhuman primates (NHPs) received an emergency compassionate use exception from the FDA for the treatment of 2 American medical workers infected with EBOV, and they are currently virus free and recovering.

  11. Recombinant measles virus vaccine expressing the Nipah virus glycoprotein protects against lethal Nipah virus challenge.

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    Misako Yoneda

    Full Text Available Nipah virus (NiV is a member of the genus Henipavirus, which emerged in Malaysia in 1998. In pigs, infection resulted in a predominantly non-lethal respiratory disease; however, infection in humans resulted in over 100 deaths. Nipah virus has continued to re-emerge in Bangladesh and India, and person-to-person transmission appeared in the outbreak. Although a number of NiV vaccine studies have been reported, there are currently no vaccines or treatments licensed for human use. In this study, we have developed a recombinant measles virus (rMV vaccine expressing NiV envelope glycoproteins (rMV-HL-G and rMV-Ed-G. Vaccinated hamsters were completely protected against NiV challenge, while the mortality of unvaccinated control hamsters was 90%. We trialed our vaccine in a non-human primate model, African green monkeys. Upon intraperitoneal infection with NiV, monkeys showed several clinical signs of disease including severe depression, reduced ability to move and decreased food ingestion and died at 7 days post infection (dpi. Intranasal and oral inoculation induced similar clinical illness in monkeys, evident around 9 dpi, and resulted in a moribund stage around 14 dpi. Two monkeys immunized subcutaneously with rMV-Ed-G showed no clinical illness prior to euthanasia after challenge with NiV. Viral RNA was not detected in any organ samples collected from vaccinated monkeys, and no pathological changes were found upon histopathological examination. From our findings, we propose that rMV-NiV-G is an appropriate NiV vaccine candidate for use in humans.

  12. Recombinant measles virus vaccine expressing the Nipah virus glycoprotein protects against lethal Nipah virus challenge.

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    Yoneda, Misako; Georges-Courbot, Marie-Claude; Ikeda, Fusako; Ishii, Miho; Nagata, Noriyo; Jacquot, Frederic; Raoul, Hervé; Sato, Hiroki; Kai, Chieko

    2013-01-01

    Nipah virus (NiV) is a member of the genus Henipavirus, which emerged in Malaysia in 1998. In pigs, infection resulted in a predominantly non-lethal respiratory disease; however, infection in humans resulted in over 100 deaths. Nipah virus has continued to re-emerge in Bangladesh and India, and person-to-person transmission appeared in the outbreak. Although a number of NiV vaccine studies have been reported, there are currently no vaccines or treatments licensed for human use. In this study, we have developed a recombinant measles virus (rMV) vaccine expressing NiV envelope glycoproteins (rMV-HL-G and rMV-Ed-G). Vaccinated hamsters were completely protected against NiV challenge, while the mortality of unvaccinated control hamsters was 90%. We trialed our vaccine in a non-human primate model, African green monkeys. Upon intraperitoneal infection with NiV, monkeys showed several clinical signs of disease including severe depression, reduced ability to move and decreased food ingestion and died at 7 days post infection (dpi). Intranasal and oral inoculation induced similar clinical illness in monkeys, evident around 9 dpi, and resulted in a moribund stage around 14 dpi. Two monkeys immunized subcutaneously with rMV-Ed-G showed no clinical illness prior to euthanasia after challenge with NiV. Viral RNA was not detected in any organ samples collected from vaccinated monkeys, and no pathological changes were found upon histopathological examination. From our findings, we propose that rMV-NiV-G is an appropriate NiV vaccine candidate for use in humans.

  13. Comparison of the Pathogenicity of Nipah Virus Isolates from Bangladesh and Malaysia in the Syrian Hamster

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    DeBuysscher, Blair L.; Emmie de Wit; Munster, Vincent J.; Dana Scott; Heinz Feldmann; Joseph Prescott

    2013-01-01

    Nipah virus is a zoonotic pathogen that causes severe disease in humans. The mechanisms of pathogenesis are not well described. The first Nipah virus outbreak occurred in Malaysia, where human disease had a strong neurological component. Subsequent outbreaks have occurred in Bangladesh and India and transmission and disease processes in these outbreaks appear to be different from those of the Malaysian outbreak. Until this point, virtually all Nipah virus studies in vitro and in vivo, includi...

  14. Detection of human metapneumovirus from children with acute otitis media.

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    Suzuki, Akira; Watanabe, Oshi; Okamoto, Michiko; Endo, Hiroko; Yano, Hisakazu; Suetake, Mitsuko; Nishimura, Hidekazu

    2005-07-01

    Nasal and middle ear specimens collected from children with acute otitis media were subjected to viral isolation and bacteria culture. All virus-negative specimens underwent reverse transcription polymerase chain reaction to detect human metapneumovirus. Three of 126 middle ear specimens were positive by this assay.

  15. Biochemical characterization of the small hydrophobic protein of avian metapneumovirus

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    Avian metapneumovirus (aMPV) is a paramyxovirus that has three membrane-associate proteins: glycoprotein (G), fusion (F), and small hydrophobic (SH) proteins. Among them, the SH protein is a small type II integral membrane protein that is incorporated into virions and is only present in certain para...

  16. Subclinical infection without encephalitis in mice following intranasal exposure to Nipah virus-Malaysia and Nipah virus-Bangladesh

    OpenAIRE

    Dups, Johanna; Middleton, Deborah; Long, Fenella; Arkinstall, Rachel; Glenn A Marsh; Wang, Lin-Fa

    2014-01-01

    Background Nipah virus and Hendra virus are closely related and following natural or experimental exposure induce similar clinical disease. In humans, encephalitis is the most serious outcome of infection and, hitherto, research into the pathogenesis of henipavirus encephalitis has been limited by the lack of a suitable model. Recently we reported a wild-type mouse model of Hendra virus (HeV) encephalitis that should facilitate detailed investigations of its neuropathogenesis, including mecha...

  17. Epidemiology, surveillance and control of Nipah virus infections in Malaysia.

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    Chua, K B

    2010-12-01

    The outbreak of Nipah virus, affecting pigs and pig-farm workers, was first noted in September 1998 in the north-western part of peninsular Malaysia. By March 1999, the outbreak had spread to other pig-farming areas of the country, inclusive of the neighbouring country, Singapore. A total of 283 human cases of viral encephalitis with 109 deaths were recorded in Malaysia from 29 September 1998 to December 1999. During the outbreak period, a number of surveillances under three broad groups; Surveillance in Human Health Sector, Surveillance in Animal Health Sector, and Surveillance for the Reservoir Hosts, were carried out to determine the prevalence, risk of virus infections and transmission in human and swine populations as well as the source and reservoir hosts of Nipah virus. Surveillance data showed that the virus spread rapidly among pigs within infected farms and transmission was attributed to direct contact with infective excretions and secretions. The spread of the virus among pig farms within and between states of peninsular Malaysia was due to movement of pigs. The transmission of the virus to humans was through close contact with infected pigs. Human to human transmission was considered a rare event though the Nipah virus could be isolated from saliva, urine, nasal and pharyngeal secretions of patients. Field investigations identified fruitbats of the Pteropid species as the natural reservoir hosts of the viruses. The outbreak was effectively brought under control following the discovery of the virus and institution of correct control measures through a combined effort of multi-ministerial and multidisciplinary teams working in close co-operation and collaboration with other international agencies.

  18. Specificity and functional interaction of the polymerase complex proteins of human and avian metapneumoviruses

    NARCIS (Netherlands)

    M.T. de Graaf (Marieke); S. Herfst (Sander); E.J.A. Schrauwen (Eefje); Y. Choi (Ying); B.G. van den Hoogen (Bernadette); A.D.M.E. Osterhaus (Albert); R.A.M. Fouchier (Ron)

    2008-01-01

    textabstractHuman metapneumovirus (HMPV) and avian metapneumovirus (AMPV) have a similar genome organization and protein composition, but a different host range. AMPV subgroup C (AMPV-C) is more closely relaled to HMPV than other AMPVs. To investigate the specificity and functional interaction of th

  19. Rational design of avian metapneumovirus live attenuated vaccines by inhibiting viral messenger RNA cap methyltransferase

    Science.gov (United States)

    Avian metapneumovirus (aMPV), also known as avian pneumovirus or turkey rhinotracheitis, is a non-segmented negative-sense RNA virus belonging to the family of Paramyxoviridae, the subfamily Pneumovirinae, and the genus Metapneumovirus. aMPV is the causative agent of respiratory tract infection and ...

  20. Subclinical infection without encephalitis in mice following intranasal exposure to Nipah virus-Malaysia and Nipah virus-Bangladesh.

    Science.gov (United States)

    Dups, Johanna; Middleton, Deborah; Long, Fenella; Arkinstall, Rachel; Marsh, Glenn A; Wang, Lin-Fa

    2014-06-02

    Nipah virus and Hendra virus are closely related and following natural or experimental exposure induce similar clinical disease. In humans, encephalitis is the most serious outcome of infection and, hitherto, research into the pathogenesis of henipavirus encephalitis has been limited by the lack of a suitable model. Recently we reported a wild-type mouse model of Hendra virus (HeV) encephalitis that should facilitate detailed investigations of its neuropathogenesis, including mechanisms of disease recrudescence. In this study we investigated the possibility of developing a similar model of Nipah virus encephalitis. Aged and young adult wild type mice did not develop clinical disease including encephalitis following intranasal exposure to either the Malaysia (NiV-MY) or Bangladesh (NiV-BD) strains of Nipah virus. However viral RNA was detected in lung tissue of mice at euthanasia (21 days following exposure) accompanied by a non-neutralizing antibody response. In a subsequent time course trial this viral RNA was shown to be reflective of an earlier self-limiting and subclinical lower respiratory tract infection through successful virus re-isolation and antigen detection in lung. There was no evidence for viremia or infection of other organs, including brain. Mice develop a subclinical self-limiting lower respiratory tract infection but not encephalitis following intranasal exposure to NiV-BD or NiV-MY. These results contrast with those reported for HeV under similar exposure conditions in mice, demonstrating a significant biological difference in host clinical response to exposure with these viruses. This finding provides a new platform from which to explore the viral and/or host factors that determine the neuroinvasive ability of henipaviruses.

  1. Nipah virus in the fruit bat Pteropus vampyrus in Sumatera, Indonesia.

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    Indrawati Sendow

    Full Text Available Nipah virus causes periodic livestock and human disease with high case fatality rate, and consequent major economic, social and psychological impacts. Fruit bats of the genus Pteropus are the natural reservoir. In this study, we used real time PCR to screen the saliva and urine of P. vampyrus from North Sumatera for Nipah virus genome. A conventional reverse transcriptase (RT-PCR assay was used on provisionally positive samples to corroborate findings. This is the first report of Nipah virus detection in P. vampyrus in Sumatera, Indonesia.

  2. A BSL-4 High-Throughput Screen Identifies Sulfonamide Inhibitors of Nipah Virus

    OpenAIRE

    2014-01-01

    Nipah virus is a biosafety level 4 (BSL-4) pathogen that causes severe respiratory illness and encephalitis in humans. To identify novel small molecules that target Nipah virus replication as potential therapeutics, Southern Research Institute and Galveston National Laboratory jointly developed an automated high-throughput screening platform that is capable of testing 10,000 compounds per day within BSL-4 biocontainment. Using this platform, we screened a 10,080-compound library using a cell-...

  3. Lessons from the Nipah virus outbreak in Malaysia.

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    Looi, Lai-Meng; Chua, Kaw-Bing

    2007-12-01

    The Nipah virus outbreak in Malaysia (September 1998 to May 1999) resulted in 265 cases of acute encephalitis with 105 deaths, and near collapse of the billion-dollar pig-farming industry. Because it was initially attributed to Japanese encephalitis, early control measures were ineffective, and the outbreak spread to other parts of Malaysia and nearby Singapore. The isolation of the novel aetiological agent, the Nipah virus (NiV), from the cerebrospinal fluid of an outbreak victim was the turning point which led to outbreak control 2 months later. Together with the Hendra virus, NiV is now recognised as a new genus, Henipavirus (Hendra + Nipah), in the Paramyxoviridae family. Efforts of the local and international scientific community have since elucidated the epidemiology, clinico-pathophysiology and pathogenesis of this new disease. Humans contracted the infection from close contact with infected pigs, and formed the basis for pig-culling that eventually stopped the outbreak. NiV targeted medium-sized and small blood vessels resulting in endothelial multinucleated syncytia and fibrinoid necrosis. Autopsies revealed disseminated cerebral microinfarctions resulting from vasculitis-induced thrombosis and direct neuronal involvement. The discovery of NiV in the urine and saliva of Malaysian Island flying foxes (Pteropus hypomelanus and Petropus vampyrus) implicated these as natural reservoir hosts of NiV. It is probable that initial transmission of NiV from bats to pigs occurred in late 1997/early 1998 through contamination of pig swill by bat excretions, as a result of migration of these forest fruitbats to cultivated orchards and pig-farms, driven by fruiting failure of forest trees during the El Nino-related drought and anthropogenic fires in Indonesia in 1997-1998. This outbreak emphasizes the need for sharing information of any unusual illnesses in animals and humans, an open-minded approach and close collaboration and co-ordination between the medical

  4. A treatment for and vaccine against the deadly Hendra and Nipah viruses.

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    Broder, Christopher C; Xu, Kai; Nikolov, Dimitar B; Zhu, Zhongyu; Dimitrov, Dimiter S; Middleton, Deborah; Pallister, Jackie; Geisbert, Thomas W; Bossart, Katharine N; Wang, Lin-Fa

    2013-10-01

    Hendra virus and Nipah virus are bat-borne paramyxoviruses that are the prototypic members of the genus Henipavirus. The henipaviruses emerged in the 1990s, spilling over from their natural bat hosts and causing serious disease outbreaks in humans and livestock. Hendra virus emerged in Australia and since 1994 there have been 7 human infections with 4 case fatalities. Nipah virus first appeared in Malaysia and subsequent outbreaks have occurred in Bangladesh and India. In total, there have been an estimated 582 human cases of Nipah virus and of these, 54% were fatal. Their broad species tropism and ability to cause fatal respiratory and/or neurologic disease in humans and animals make them important transboundary biological threats. Recent experimental findings in animals have demonstrated that a human monoclonal antibody targeting the viral G glycoprotein is an effective post-exposure treatment against Hendra and Nipah virus infection. In addition, a subunit vaccine based on the G glycoprotein of Hendra virus affords protection against Hendra and Nipah virus challenge. The vaccine has been developed for use in horses in Australia and is the first vaccine against a Biosafety Level-4 (BSL-4) agent to be licensed and commercially deployed. Together, these advances offer viable approaches to address Hendra and Nipah virus infection of livestock and people.

  5. Nipah virus matrix protein: expert hacker of cellular machines.

    Science.gov (United States)

    Watkinson, Ruth E; Lee, Benhur

    2016-08-01

    Nipah virus (NiV, Henipavirus) is a highly lethal emergent zoonotic paramyxovirus responsible for repeated human outbreaks of encephalitis in South East Asia. There are no approved vaccines or treatments, thus improved understanding of NiV biology is imperative. NiV matrix protein recruits a plethora of cellular machinery to scaffold and coordinate virion budding. Intriguingly, matrix also hijacks cellular trafficking and ubiquitination pathways to facilitate transient nuclear localization. While the biological significance of matrix nuclear localization for an otherwise cytoplasmic virus remains enigmatic, the molecular details have begun to be characterized, and are conserved among matrix proteins from divergent paramyxoviruses. Matrix protein appropriation of cellular machinery will be discussed in terms of its early nuclear targeting and later role in virion assembly.

  6. The Role of the Hendra Virus and Nipah Virus Attachment Glycoproteins in Receptor Binding and Antibody Neutralization

    Science.gov (United States)

    2014-01-31

    Patz JA, et al. 2013. The Role of Landscape Composition and Configuration on Pteropus giganteus Roosting Ecology and Nipah Virus Spillover Risk in...Middleton D, Crameri G, Bingham J, Halpin K, et al. 2006. Feline model of acute nipah virus infection and protection with a soluble glycoprotein

  7. Nipah病毒性脑炎的研究进展%Progression of Nipah Virus Encephalitis

    Institute of Scientific and Technical Information of China (English)

    左联; 徐霞红

    2012-01-01

    Nipah病毒是近年在东南亚新出现的一种人畜共患病病毒,导致Nipah病毒性脑炎的暴发流行,传染性强,死亡率高.本文主要就近年对Nipah病毒性脑炎的研究进展包括病毒分子生物学、发病机制、流行病学、临床及辅助检查等作一综述.%Nipah virus is a newly zoonotic disease virus emerged in Southeast Asia. Nipah virus infection mainly leads to the outbreak of encephalitis, with highly infectious and mortality. This article aim to review progression of Nipah virus in recent years which include viral molecular biology, pathogenesis, epidemiology, clinical and auxiliary examination,etc.

  8. Sequence analysis of the large polymerase (L) protein of the US strain of avian metapneumovirus indicates a close resemblance to that of the human metapneumovirus.

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    Govindarajan, Dhanasekaran; Samal, Siba K

    2004-09-15

    The complete nucleotide sequence of the large polymerase (L) protein of the avian metapneumovirus subgroup C strain Colorado was determined. The L protein gene of avian pneumovirus Colorado isolate (APV-C) was 6173 nucleotides in length from the gene-start to the gene-end and encoded a polypeptide of 2005 amino acids in length. The length of the L protein of APV-C was exactly the same as that of human metapneumovirus (hMPV) and one amino acid longer than the L protein of APV subgroup A. The L protein of APV-C showed 80% amino acid identity with the L protein of hMPV, but only 64% amino acid identity with the L protein of APV-A. The nucleotide and deduced amino acid sequences were compared with the corresponding sequences of eleven other paramyxoviruses. All six domains characteristic of paramyxovirus L proteins were also observed in the L protein of APV-C. All the polymerase core motifs in domain III were conserved to nearly 100% in the metapneumoviruses. Similarly, the putative ATP-binding motif in domain VI was completely conserved among the metapneumoviruses and differed in length, by one intermediate residue, from other paramyxoviruses. Phylogenetic analysis of the different L proteins also revealed a closer relationship between APV-C and hMPV.

  9. Roosting behaviour and habitat selection of Pteropus giganteus reveals potential links to Nipah virus epidemiology.

    Science.gov (United States)

    Hahn, Micah B; Epstein, Jonathan H; Gurley, Emily S; Islam, Mohammad S; Luby, Stephen P; Daszak, Peter; Patz, Jonathan A

    2014-04-01

    1. Flying foxes Pteropus spp. play a key role in forest regeneration as seed dispersers and are also the reservoir of many viruses, including Nipah virus in Bangladesh. Little is known about their habitat requirements, particularly in South Asia. Identifying Pteropus habitat preferences could assist in understanding the risk of zoonotic disease transmission broadly, and in Bangladesh, could help explain the spatial distribution of human Nipah virus cases. 2. We analysed characteristics of Pteropus giganteus roosts and constructed an ecological niche model to identify suitable habitat in Bangladesh. We also assessed the distribution of suitable habitat in relation to the location of human Nipah virus cases. 3. Compared to non-roost trees, P. giganteus roost trees are taller with larger diameters, and are more frequently canopy trees. Colony size was larger in densely forested regions and smaller in flood-affected areas. Roosts were located in areas with lower annual precipitation and higher human population density than non-roost sites. 4. We predicted that 2-17% of Bangladesh's land area is suitable roosting habitat. Nipah virus outbreak villages were 2.6 times more likely to be located in areas predicted as highly suitable habitat for P. giganteus compared to non-outbreak villages. 5. Synthesis and applications. Habitat suitability modelling may help identify previously undocumented Nipah outbreak locations and improve our understanding of Nipah virus ecology by highlighting regions where there is suitable bat habitat but no reported human Nipah virus. Conservation and public health education is a key component of P. giganteus management in Bangladesh due to the general misunderstanding and fear of bats that are a reservoir of Nipah virus. Affiliation between Old World fruit bats (Pteropodidae) and people is common throughout their range, and in order to conserve these keystone bat species and prevent emergence of zoonotic viruses, it is imperative that we

  10. Vaccine potential of Nipah virus-like particles.

    Directory of Open Access Journals (Sweden)

    Pramila Walpita

    Full Text Available Nipah virus (NiV was first recognized in 1998 in a zoonotic disease outbreak associated with highly lethal febrile encephalitis in humans and a predominantly respiratory disease in pigs. Periodic deadly outbreaks, documentation of person-to-person transmission, and the potential of this virus as an agent of agroterror reinforce the need for effective means of therapy and prevention. In this report, we describe the vaccine potential of NiV virus-like particles (NiV VLPs composed of three NiV proteins G, F and M. Co-expression of these proteins under optimized conditions resulted in quantifiable amounts of VLPs with many virus-like/vaccine desirable properties including some not previously described for VLPs of any paramyxovirus: The particles were fusogenic, inducing syncytia formation; PCR array analysis showed NiV VLP-induced activation of innate immune defense pathways; the surface structure of NiV VLPs imaged by cryoelectron microscopy was dense, ordered, and repetitive, and consistent with similarly derived structure of paramyxovirus measles virus. The VLPs were composed of all the three viral proteins as designed, and their intracellular processing also appeared similar to NiV virions. The size, morphology and surface composition of the VLPs were consistent with the parental virus, and importantly, they retained their antigenic potential. Finally, these particles, formulated without adjuvant, were able to induce neutralizing antibody response in Balb/c mice. These findings indicate vaccine potential of these particles and will be the basis for undertaking future protective efficacy studies in animal models of NiV disease.

  11. Human neuronal cell protein responses to Nipah virus infection

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    Hassan Sharifah

    2007-06-01

    Full Text Available Abstract Background Nipah virus (NiV, a recently discovered zoonotic virus infects and replicates in several human cell types. Its replication in human neuronal cells, however, is less efficient in comparison to other fully susceptible cells. In the present study, the SK-N-MC human neuronal cell protein response to NiV infection is examined using proteomic approaches. Results Method for separation of the NiV-infected human neuronal cell proteins using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE was established. At least 800 protein spots were resolved of which seven were unique, six were significantly up-regulated and eight were significantly down-regulated. Six of these altered proteins were identified using mass spectrometry (MS and confirmed using MS/MS. The heterogenous nuclear ribonucleoprotein (hnRNP F, guanine nucleotide binding protein (G protein, voltage-dependent anion channel 2 (VDAC2 and cytochrome bc1 were present in abundance in the NiV-infected SK-N-MC cells in contrast to hnRNPs H and H2 that were significantly down-regulated. Conclusion Several human neuronal cell proteins that are differentially expressed following NiV infection are identified. The proteins are associated with various cellular functions and their abundance reflects their significance in the cytopathologic responses to the infection and the regulation of NiV replication. The potential importance of the ratio of hnRNP F, and hnRNPs H and H2 in regulation of NiV replication, the association of the mitochondrial protein with the cytopathologic responses to the infection and induction of apoptosis are highlighted.

  12. Increase human metapneumovirus mediated morbidity following pandemic influenza infection.

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    Liora Regev

    Full Text Available Human metapneumovirus (hMPV is a recently discovered respiratory pathogen, infecting mainly young children. The infected patients suffer from influenza like symptoms (ILS. In Israel the virus is mainly circulating in February to March. Here we report on an increased rate of hMPV infection in the winter season of 2009-10. The 2009-10 infection had several unique characteristics when compared to previous seasons; it started around January and a large number of infants were infected by the virus. Genetic analysis based on the viral L and F genes of hMPV showed that only subtypes A2 and B2 circulated in Israel. Additionally, we have identified a novel variant of hMPV within subgroup A2b, which subdivide it into A2b1 and A2b2. Finally, we showed that the hMPV infection was detected in the country soon after the infection with the pandemic influenza virus had declined, that infection with the pandemic influenza virus was dominant and that it interfered with the infection of other respiratory viruses. Thus, we suggest that the unusual increase in hMPV infection observed in 2009-10 was due to the appearance of the pandemic influenza virus in the winter season prior to 2009-10.

  13. Relevance of human metapneumovirus in exacerbations of COPD

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    Bauer TT

    2005-12-01

    Full Text Available Abstract Background and methods Human metapneumovirus (hMPV is a recently discovered respiratory virus associated with bronchiolitis, pneumonia, croup and exacerbations of asthma. Since respiratory viruses are frequently detected in patients with acute exacerbations of COPD (AE-COPD it was our aim to investigate the frequency of hMPV detection in a prospective cohort of hospitalized patients with AE-COPD compared to patients with stable COPD and to smokers without by means of quantitative real-time RT-PCR. Results We analysed nasal lavage and induced sputum of 130 patients with AE-COPD, 65 patients with stable COPD and 34 smokers without COPD. HMPV was detected in 3/130 (2.3% AE-COPD patients with a mean of 6.5 × 105 viral copies/ml in nasal lavage and 1.88 × 105 viral copies/ml in induced sputum. It was not found in patients with stable COPD or smokers without COPD. Conclusion HMPV is only found in a very small number of patients with AE-COPD. However it should be considered as a further possible viral trigger of AE-COPD because asymptomatic carriage is unlikely.

  14. Serologic evidence of human metapneumovirus circulation in Uruguay

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    Santiago Mirazo

    2005-11-01

    Full Text Available First identified in 2001, the human metapneumovirus (hMPV, is a respiratory tract pathogen that affects young children, elderly, and immunocompromised patients. The present work represents the first serologic study carried out in Uruguay. It was performed with the purpose of obtaining serological evidence of hMPV circulation in Uruguay and to contribute to the few serologic reports described until now. Sixty nine serum samples collected between 1998 and 2001 by vein puncture from patients without respiratory symptoms or underlying pathology aged 6 days to 60 years were examined using an indirect immunofluorescence assay (IFA. The global seropositivity rate of the samples was 80% (55/69. Rates of 60% (15/25 and 91% (40/44 were observed for the pediatric and adult cohorts, respectively. Results obtained from a longitudinal analysis of 6 children aged 6 days to 18 months are discussed. These results are a clear evidence of hMPV circulation in Uruguay, at least since 1998, and reinforce the previous data on worldwide circulation of this virus.

  15. Molecular Epidemiology of Human Metapneumovirus in Riyadh Province, Saudi Arabia.

    Science.gov (United States)

    Amer, Haitham Mohamed

    2016-01-01

    Human metapneumovirus (HMPV) is an important cause of respiratory tract illness in children. Two HMPV subgroups, A and B, and four genotypes, A1, A2, B1 and B2, have been identified. Concurrent circulation of the different genotypes in yearly epidemics has been recorded globally, but not in Saudi Arabia. The current report was designed to study HMPV epidemiology in Saudi children and to analyze the genetic diversity and circulation patterns. Nasopharyngeal aspirates (n = 174) were collected from hospitalized children in Riyadh (2008-2009). The screening of samples using real-time RT-PCR identified 19 HMPV strains. The majority of the strains belonged to subgroup B, while all strains of subgroup A were members of genotype A2. In 2008, only subgroup B was recognized, whereas in 2009 both subgroups were identified to be cocirculating at similar rates. The full-length attachment (G) gene and a partial sequence of the fusion (F) gene of positive samples were sequenced. The G gene showed a high degree of genetic diversity and exhibited a variable number of positively selected sites in different lineages. In contrast, the F gene demonstrated an extensive genetic stability with a higher tendency toward purifying selection. This is the first report on HMPV genotype circulation in Saudi Arabia; however, the exact circulation kinetics requires further retrospective and prospective study.

  16. Molecular epidemiology and phylogeny of Nipah virus infection: A mini review.

    Science.gov (United States)

    Angeletti, Silvia; Lo Presti, Alessandra; Cella, Eleonora; Ciccozzi, Massimo

    2016-07-01

    Nipah virus (NiV) is a member of the genus Henipavirus of the family Paramyxoviridae, characterized by high pathogenicity and endemic in South Asia. It is classified as a Biosafety Level-4 (BSL-4) agent. The case-fatality varies from 40% to 70% depending on the severity of the disease and on the availability of adequate healthcare facilities. At present no antiviral drugs are available for NiV disease and the treatment is just supportive. Phylogenetic and evolutionary analyses can be used to help in understanding the epidemiology and the temporal origin of this virus. This review provides an overview of evolutionary studies performed on Nipah viruses circulating in different countries. Thirty phylogenetic studies have been published from 2000 to 2015 years, searching on pub-med using the key words 'Nipah virus AND phylogeny' and twenty-eight molecular epidemiological studies from 2006 to 2015 have been performed, typing the key words 'Nipah virus AND molecular epidemiology'. Overall data from the published study demonstrated as phylogenetic and evolutionary analysis represent promising tools to evidence NiV epidemics, to study their origin and evolution and finally to act with effective preventive measure.

  17. A cohort study of health care workers to assess nosocomial transmissibility of Nipah virus, Malaysia, 1999.

    Science.gov (United States)

    Mounts, A W; Kaur, H; Parashar, U D; Ksiazek, T G; Cannon, D; Arokiasamy, J T; Anderson, L J; Lye, M S

    2001-03-01

    During 1998-1999, an outbreak of Nipah virus encephalitis occurred in Malaysia. To assess the possibility of nosocomial transmission, 338 health care workers (HCWs) exposed and 288 HCWs unexposed to outbreak-related patients were surveyed, and their serum samples were tested for anti-Nipah virus antibody. Needlestick injuries were reported by 12 (3%) HCWs, mucosal surface exposure to body fluids by 39 (11%), and skin exposure to body fluids by 89 (25%). No encephalitis occurred in either group. Three exposed and no unexposed HCWs tested positive by EIA for IgG antibodies. It is likely that these 3 were false positives; no IgM response occurred, and the serum samples were negative for anti-Nipah virus neutralizing antibodies. The risk of nosocomial transmission of Nipah virus appears to be low; however, given the high case-fatality rate and the presence of virus in respiratory secretions and urine of some patients, standard and droplet infection-control practices should be maintained with these patients.

  18. Brazilian avian metapneumovirus subtypes A and B: experimental infection of broilers and evaluation of vaccine efficacy

    OpenAIRE

    dos Santos, Marcia B.; Matheus C. Martini; Ferreira, Helena L.; Luciana H.A. da Silva; Paulo A. Fellipe; Spilki,Fernando R.; Arns, Clarice W.

    2012-01-01

    Santos M.B., Martini M.C., Ferreira H.L., Silva L.H.A., Fellipe P.A., Spilki F.R. & Arns C.W. 2012. Brazilian avian metapneumovirus subtypes A and B: experimental infection of broilers and evaluation of vaccine efficacy. Pesquisa Veterinaria Brasileira 32(12):1257-1262. Laboratorio de Virologia, Instituto de Biologia, Universidade Estadual de Campinas, Rua Monteiro Lobato s/n, Cx. Postal 6109, Campinas, SP 13083-970, Brazil. E-mail: Avian metapneumovirus (aMPV) is a respirator...

  19. Comparison of the pathogenicity of Nipah virus isolates from Bangladesh and Malaysia in the Syrian hamster.

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    Blair L DeBuysscher

    Full Text Available Nipah virus is a zoonotic pathogen that causes severe disease in humans. The mechanisms of pathogenesis are not well described. The first Nipah virus outbreak occurred in Malaysia, where human disease had a strong neurological component. Subsequent outbreaks have occurred in Bangladesh and India and transmission and disease processes in these outbreaks appear to be different from those of the Malaysian outbreak. Until this point, virtually all Nipah virus studies in vitro and in vivo, including vaccine and pathogenesis studies, have utilized a virus isolate from the original Malaysian outbreak (NiV-M. To investigate potential differences between NiV-M and a Nipah virus isolate from Bangladesh (NiV-B, we compared NiV-M and NiV-B infection in vitro and in vivo. In hamster kidney cells, NiV-M-infection resulted in extensive syncytia formation and cytopathic effects, whereas NiV-B-infection resulted in little to no morphological changes. In vivo, NiV-M-infected Syrian hamsters had accelerated virus replication, pathology and death when compared to NiV-B-infected animals. NiV-M infection also resulted in the activation of host immune response genes at an earlier time point. Pathogenicity was not only a result of direct effects of virus replication, but likely also had an immunopathogenic component. The differences observed between NiV-M and NiV-B pathogeneis in hamsters may relate to differences observed in human cases. Characterization of the hamster model for NiV-B infection allows for further research of the strain of Nipah virus responsible for the more recent outbreaks in humans. This model can be used to study NiV-B pathogenesis, transmission, and countermeasures that could be used to control outbreaks.

  20. A human lung xenograft mouse model of Nipah virus infection.

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    Gustavo Valbuena

    2014-04-01

    Full Text Available Nipah virus (NiV is a member of the genus Henipavirus (family Paramyxoviridae that causes severe and often lethal respiratory illness and encephalitis in humans with high mortality rates (up to 92%. NiV can cause Acute Lung Injury (ALI in humans, and human-to-human transmission has been observed in recent outbreaks of NiV. While the exact route of transmission to humans is not known, we have previously shown that NiV can efficiently infect human respiratory epithelial cells. The molecular mechanisms of NiV-associated ALI in the human respiratory tract are unknown. Thus, there is an urgent need for models of henipavirus infection of the human respiratory tract to study the pathogenesis and understand the host responses. Here, we describe a novel human lung xenograft model in mice to study the pathogenesis of NiV. Following transplantation, human fetal lung xenografts rapidly graft and develop mature structures of adult lungs including cartilage, vascular vessels, ciliated pseudostratified columnar epithelium, and primitive "air" spaces filled with mucus and lined by cuboidal to flat epithelium. Following infection, NiV grows to high titers (10(7 TCID50/gram lung tissue as early as 3 days post infection (pi. NiV targets both the endothelium as well as respiratory epithelium in the human lung tissues, and results in syncytia formation. NiV infection in the human lung results in the production of several cytokines and chemokines including IL-6, IP-10, eotaxin, G-CSF and GM-CSF on days 5 and 7 pi. In conclusion, this study demonstrates that NiV can replicate to high titers in a novel in vivo model of the human respiratory tract, resulting in a robust inflammatory response, which is known to be associated with ALI. This model will facilitate progress in the fundamental understanding of henipavirus pathogenesis and virus-host interactions; it will also provide biologically relevant models for other respiratory viruses.

  1. Affinity selection of Nipah and Hendra virus-related vaccine candidates from a complex random peptide library displayed on bacteriophage virus-like particles

    Energy Technology Data Exchange (ETDEWEB)

    Peabody, David S.; Chackerian, Bryce; Ashley, Carlee; Carnes, Eric; Negrete, Oscar

    2017-01-24

    The invention relates to virus-like particles of bacteriophage MS2 (MS2 VLPs) displaying peptide epitopes or peptide mimics of epitopes of Nipah Virus envelope glycoprotein that elicit an immune response against Nipah Virus upon vaccination of humans or animals. Affinity selection on Nipah Virus-neutralizing monoclonal antibodies using random sequence peptide libraries on MS2 VLPs selected peptides with sequence similarity to peptide sequences found within the envelope glycoprotein of Nipah itself, thus identifying the epitopes the antibodies recognize. The selected peptide sequences themselves are not necessarily identical in all respects to a sequence within Nipah Virus glycoprotein, and therefore may be referred to as epitope mimics VLPs displaying these epitope mimics can serve as vaccine. On the other hand, display of the corresponding wild-type sequence derived from Nipah Virus and corresponding to the epitope mapped by affinity selection, may also be used as a vaccine.

  2. Analysis of the genomic sequence of a human metapneumovirus (hMPV).

    NARCIS (Netherlands)

    B.G. van den Hoogen (Bernadette); T.M. Bestebroer (Theo); A.D.M.E. Osterhaus (Albert); R.A.M. Fouchier (Ron)

    2002-01-01

    textabstractWe recently described the isolation of a novel paramyxovirus from children with respiratory tract disease in The Netherlands. Based on biological properties and limited sequence information the virus was provisionally classified as the first nonavian member of the Metapneumovirus genus a

  3. Generation of recombinant avian metapneumovirus subgroup C viruses for pathogenesis studies and vaccine development

    Science.gov (United States)

    Avian metapneumovirus subgroup C (aMPV-C), a member of the Paramyxoviridae family, causes an upper respiratory disease in turkeys, resulting in significant economic losses for the US turkey industry. To study the disease pathogenesis and to eventually develop a safe and effective vaccine against aMP...

  4. Human metapneumovirus and respiratory syncytial virus in hospitalized danish children with acute respiratory tract infection

    DEFF Research Database (Denmark)

    von Linstow, Marie-Louise; Henrik Larsen, Hans; Koch, Anders;

    2004-01-01

    The newly discovered human metapneumovirus (hMPV) has been shown to be associated with respiratory illness. We determined the frequencies and clinical features of hMPV and respiratory syncytial virus (RSV) infections in 374 Danish children with 383 episodes of acute respiratory tract infection...

  5. Absence of human metapneumovirus co-infection in cases of severe respiratory syncytial virus infection

    NARCIS (Netherlands)

    van Woensel, J B M; Bos, A P; Lutter, R; Rossen, J W A; Schuurman, R

    2006-01-01

    It has been suggested that co-infection of human metapneumovirus (hMPV) in severe respiratory syncytial (RSV) virus bronchiolitis is very common. To evaluate the epidemiology of hMPV co-infection in children with severe lower respiratory tract infection caused by RSV virus. This was an observational

  6. Topology and cellular localization of the small hydrophobic protein of avian metapneumovirus

    Science.gov (United States)

    The small hydrophobic protein (SH) is a type II integral membrane protein that is packaged into virions and is only present in certain paramyxoviruses including metapneumovirus. In addition to a highly divergent primary sequence, SH proteins vary significantly in size among the different viruses. Hu...

  7. Severe respiratory failure due to co-infection with human metapneumovirus and Streptococcus pneumoniae

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    Masafumi Seki

    2014-01-01

    Full Text Available A 64-year-old male patient was admitted with respiratory failure, although chest X-rays revealed only mild bronchiolitis. Streptococcus pneumoniae, which usually presents as massive lobular pneumonia, was isolated from sputum, however, pan-pathogen screening using a next-generation sequencer also detected human metapneumovirus genome fragments.

  8. A neutralizing human monoclonal antibody protects against lethal disease in a new ferret model of acute nipah virus infection.

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    Katharine N Bossart

    2009-10-01

    Full Text Available Nipah virus is a broadly tropic and highly pathogenic zoonotic paramyxovirus in the genus Henipavirus whose natural reservoirs are several species of Pteropus fruit bats. Nipah virus has repeatedly caused outbreaks over the past decade associated with a severe and often fatal disease in humans and animals. Here, a new ferret model of Nipah virus pathogenesis is described where both respiratory and neurological disease are present in infected animals. Severe disease occurs with viral doses as low as 500 TCID(50 within 6 to 10 days following infection. The underlying pathology seen in the ferret closely resembles that seen in Nipah virus infected humans, characterized as a widespread multisystemic vasculitis, with virus replicating in highly vascular tissues including lung, spleen and brain, with recoverable virus from a variety of tissues. Using this ferret model a cross-reactive neutralizing human monoclonal antibody, m102.4, targeting the henipavirus G glycoprotein was evaluated in vivo as a potential therapeutic agent. All ferrets that received m102.4 ten hours following a high dose oral-nasal Nipah virus challenge were protected from disease while all controls died. This study is the first successful post-exposure passive antibody therapy for Nipah virus using a human monoclonal antibody.

  9. Nipah Virus Infection Outbreak with Nosocomial and Corpse-to-Human Transmission, Bangladesh

    Science.gov (United States)

    Hossain, M. Jahangir; Gurley, Emily S.; Ameen, Kazi M.H.; Parveen, Shahana; Islam, M. Saiful; Faruque, Labib I.; Podder, Goutam; Banu, Sultana S.; Lo, Michael K.; Rollin, Pierre E.; Rota, Paul A.; Daszak, Peter; Rahman, Mahmudur; Luby, Stephen P.

    2013-01-01

    Active Nipah virus encephalitis surveillance identified an encephalitis cluster and sporadic cases in Faridpur, Bangladesh, in January 2010. We identified 16 case-patients; 14 of these patients died. For 1 case-patient, the only known exposure was hugging a deceased patient with a probable case, while another case-patient’s exposure involved preparing the same corpse for burial by removing oral secretions and anogenital excreta with a cloth and bare hands. Among 7 persons with confirmed sporadic cases, 6 died, including a physician who had physically examined encephalitis patients without gloves or a mask. Nipah virus–infected patients were more likely than community-based controls to report drinking raw date palm sap and to have had physical contact with an encephalitis patient (29% vs. 4%, matched odds ratio undefined). Efforts to prevent transmission should focus on reducing caregivers’ exposure to infected patients’ bodily secretions during care and traditional burial practices. PMID:23347678

  10. Comparison of in vivo toxicity, antioxidant and immunomodulatory activities of coconut, nipah and pineapple juice vinegars.

    Science.gov (United States)

    Mohamad, Nurul Elyani; Keong Yeap, Swee; Beh, Boon Keen; Romli, Muhammad Firdaus; Yusof, Hamidah Mohd; Kristeen-Teo, Ye Wen; Sharifuddin, Shaiful Adzni; Long, Kamariah; Alitheen, Noorjahan Banu

    2017-06-20

    Vinegar is widely used as a food additive, in food preparation and as a food supplement. This study compared the phenolic acid profiles and in vivo toxicities, and antioxidant and immunomodulatory effects of coconut, nipah and pineapple juice vinegars, which were respectively prepared via a two-step fermentation using Saccharomyces cerevisiae 7013 INRA and Acetobacter aceti vat Europeans. Pineapple juice vinegar, which had the highest total phenolic acid content, also exhibited the greatest in vitro antioxidant capacity compared to coconut juice and nipah juice vinegars. Following acute and sub-chronic in vivo toxicity evaluation, no toxicity and mortality were evident and there were no significant differences in the serum biochemical profiles between mice administered the vinegars versus the control group. In the sub-chronic toxicity evaluation, the highest liver antioxidant levels were found in mice fed with pineapple juice vinegar, followed by coconut juice and nipah juice vinegars. However, compared to the pineapple juice and nipah juice vinegars, the mice fed with coconut juice vinegar, exhibited a higher population of CD4(+) and CD8(+) T-lymphocytes in the spleen, which was associated with greater levels of serum interleukin-2 and interferon-γ cytokines. Overall, the data suggested that not all vinegar samples cause acute and sub-chronic toxicity in vivo. Moreover, the in vivo immunity and organ antioxidant levels were enhanced, to varying extents, by the phenolic acids present in the vinegars. The results obtained in this study provide appropriate guidelines for further in vivo bioactivity studies and pre-clinical assessments of vinegar consumption. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  11. 尼帕病毒研究进展%Research Progress of Nipah Virus

    Institute of Scientific and Technical Information of China (English)

    张晨飞; 陈昌海; 刘耀兴

    2012-01-01

    尼帕病毒(Nipah virus,NiV)是新近发现的引发脑部炎症或呼吸道疾病等重症的新型人畜共患病病毒,该病毒最初于1999年马来西亚尼帕镇的1名脑炎患者脑脊液中分离获得,至今已经历了数次大的流行,造成严重的经济损失和人员伤亡.该病毒可由动物传播给人,也可直接人与人传播,并且能够在猪等动物身上引起严重疾病,狐蝠科的果蝠是该病毒的天然宿主.NiV感染人后的病死率极高,并且目前还没有有效的疫苗和治疗措施,生物危害性极大,被列为生物安全4级病原(BSL4).笔者从NiV的分类及分型、基因和蛋白质组、流行和分布、疫苗研制、临床和病理变化以及实验室诊断技术等方面对NiV做简单的概述.%Nipah virus is a newly identified paramyxovirus that causes serious respiratory disease and encephalitis in humans and animals. Outbreaks and spreads of this virus have led to the severe economic losses and human deaths since the first isolation of Nipah virus in Malaysia, 1999. Bats in the genus Pteropus are natural hosts of this viruses, and contact with infected animals or human-to-human transmission were thought to be the major route of the virus spreading. As is highly contagious in human and swine with high case fatality rate, Nipah virus are classified as biosafety level 4 (BSL4) agents, for which there is still no available vaccination or effective antiviral treatment. This review provides a simple overview of Nipah virus in taxonomy, genomics and proteomics, epidemiology, vaccine development, clinical and pathological signs, diagnostic techniques.

  12. Peri-exposure protection against Nipah virus disease using a single-dose recombinant vesicular stomatitis virus-based vaccine.

    Science.gov (United States)

    DeBuysscher, Blair L; Scott, Dana; Thomas, Tina; Feldmann, Heinz; Prescott, Joseph

    2016-01-01

    Nipah virus is a zoonotic paramyxovirus that causes severe disease in humans and animals. Due to almost yearly outbreaks in Bangladesh, and a large outbreak in Malaysia that lead to the shutdown of swine export, Nipah virus is both a threat to public health and the economy. Infection is associated with respiratory distress, encephalitis and human-to-human transmission, resulting in high case fatality rates during outbreaks. This study aims to address the amount of time needed until protection from a recombinant vesicular stomatitis virus-based vaccine candidate expressing the Nipah virus glycoprotein (G), which we have previously shown to protect hamsters and non-human primates when administered 28 days before challenge. We found that a single-dose vaccination, when administered 1 day before challenge, reduced viral load, limited pathology and fully protected hamsters from Nipah virus infection. The vaccine was even partially protective when administered at early time points following challenge with Nipah virus. These data indicate that a single administration of this vaccine to high-risk individuals, such as family members and health-care workers of infected patients, could be protective and useful for reducing human-to-human transmission and curbing an outbreak.

  13. Status of vaccine research and development of vaccines for Nipah virus.

    Science.gov (United States)

    Satterfield, Benjamin A; Dawes, Brian E; Milligan, Gregg N

    2016-06-03

    Nipah virus (NiV) is a highly pathogenic, recently emerged paramyxovirus that has been responsible for sporadic outbreaks of respiratory and encephalitic disease in Southeast Asia. High case fatality rates have also been associated with recent outbreaks in Malaysia and Bangladesh. Although over two billion people currently live in regions in which NiV is endemic or in which the Pteropus fruit bat reservoir is commonly found, there is no approved vaccine to protect against NiV disease. This report examines the feasibility and current efforts to develop a NiV vaccine including potential hurdles for technical and regulatory assessment of candidate vaccines and the likelihood for financing.

  14. Anthropogenic deforestation, El Niño and the emergence of Nipah virus in Malaysia.

    Science.gov (United States)

    Chua, Kaw Bing; Chua, Beng Hui; Wang, Chew Wen

    2002-06-01

    In late 1998, a novel paramyxovirus named Nipah virus, emerged in Malaysia, causing fatal disease in domestic pigs and humans with substantial economic loss to the local pig industry. Pteropid fruitbats have since been identified as a natural reservoir host. Over the last two decades, the forest habitat of these bats in Southeast Asia has been substantially reduced by deforestation for pulpwood and industrial plantation. In 1997/1998, slash-and-burn deforestation resulted in the formation of a severe haze that blanketed much of Southeast Asia in the months directly preceding the Nipah virus disease outbreak. This was exacerbated by a drought driven by the severe 1997-1998 El Niño Southern Oscillation (ENSO) event. We present data suggesting that this series of events led to a reduction in the availability of flowering and fruiting forest trees for foraging by fruitbats and culminated in unprecedented encroachment of fruitbats into cultivated fruit orchards in 1997/1998. These anthropogenic events, coupled with the location of piggeries in orchards and the design of pigsties allowed transmission of a novel paramyxovirus from its reservoir host to the domestic pig and ultimately to the human population.

  15. A BSL-4 high-throughput screen identifies sulfonamide inhibitors of Nipah virus.

    Science.gov (United States)

    Tigabu, Bersabeh; Rasmussen, Lynn; White, E Lucile; Tower, Nichole; Saeed, Mohammad; Bukreyev, Alexander; Rockx, Barry; LeDuc, James W; Noah, James W

    2014-04-01

    Nipah virus is a biosafety level 4 (BSL-4) pathogen that causes severe respiratory illness and encephalitis in humans. To identify novel small molecules that target Nipah virus replication as potential therapeutics, Southern Research Institute and Galveston National Laboratory jointly developed an automated high-throughput screening platform that is capable of testing 10,000 compounds per day within BSL-4 biocontainment. Using this platform, we screened a 10,080-compound library using a cell-based, high-throughput screen for compounds that inhibited the virus-induced cytopathic effect. From this pilot effort, 23 compounds were identified with EC50 values ranging from 3.9 to 20.0 μM and selectivities >10. Three sulfonamide compounds with EC50 values BSL-4 containment changes the paradigm for drug discovery for highly pathogenic agents because this platform can be readily modified to identify prophylactic and postexposure therapeutic candidates against other BSL-4 pathogens, particularly Ebola, Marburg, and Lassa viruses.

  16. Interdisciplinary approaches to understanding disease emergence: the past, present, and future drivers of Nipah virus emergence.

    Science.gov (United States)

    Daszak, Peter; Zambrana-Torrelio, Carlos; Bogich, Tiffany L; Fernandez, Miguel; Epstein, Jonathan H; Murray, Kris A; Hamilton, Healy

    2013-02-26

    Emerging infectious diseases (EIDs) pose a significant threat to human health, economic stability, and biodiversity. Despite this, the mechanisms underlying disease emergence are still not fully understood, and control measures rely heavily on mitigating the impact of EIDs after they have emerged. Here, we highlight the emergence of a zoonotic Henipavirus, Nipah virus, to demonstrate the interdisciplinary and macroecological approaches necessary to understand EID emergence. Previous work suggests that Nipah virus emerged due to the interaction of the wildlife reservoir (Pteropus spp. fruit bats) with intensively managed livestock. The emergence of this and other henipaviruses involves interactions among a suite of anthropogenic environmental changes, socioeconomic factors, and changes in demography that overlay and interact with the distribution of these pathogens in their wildlife reservoirs. Here, we demonstrate how ecological niche modeling may be used to investigate the potential role of a changing climate on the future risk for Henipavirus emergence. We show that the distribution of Henipavirus reservoirs, and therefore henipaviruses, will likely change under climate change scenarios, a fundamental precondition for disease emergence in humans. We assess the variation among climate models to estimate where Henipavirus host distribution is most likely to expand, contract, or remain stable, presenting new risks for human health. We conclude that there is substantial potential to use this modeling framework to explore the distribution of wildlife hosts under a changing climate. These approaches may directly inform current and future management and surveillance strategies aiming to improve pathogen detection and, ultimately, reduce emergence risk.

  17. Detailed analysis of the African green monkey model of Nipah virus disease.

    Science.gov (United States)

    Johnston, Sara C; Briese, Thomas; Bell, Todd M; Pratt, William D; Shamblin, Joshua D; Esham, Heather L; Donnelly, Ginger C; Johnson, Joshua C; Hensley, Lisa E; Lipkin, W Ian; Honko, Anna N

    2015-01-01

    Henipaviruses are implicated in severe and frequently fatal pneumonia and encephalitis in humans. There are no approved vaccines or treatments available for human use, and testing of candidates requires the use of well-characterized animal models that mimic human disease. We performed a comprehensive and statistically-powered evaluation of the African green monkey model to define parameters critical to disease progression and the extent to which they correlate with human disease. African green monkeys were inoculated by the intratracheal route with 2.5 × 10(4) plaque forming units of the Malaysia strain of Nipah virus. Physiological data captured using telemetry implants and assessed in conjunction with clinical pathology were consistent with shock, and histopathology confirmed widespread tissue involvement associated with systemic vasculitis in animals that succumbed to acute disease. In addition, relapse encephalitis was identified in 100% of animals that survived beyond the acute disease phase. Our data suggest that disease progression in the African green monkey is comparable to the variable outcome of Nipah virus infection in humans.

  18. Detailed analysis of the African green monkey model of Nipah virus disease.

    Directory of Open Access Journals (Sweden)

    Sara C Johnston

    Full Text Available Henipaviruses are implicated in severe and frequently fatal pneumonia and encephalitis in humans. There are no approved vaccines or treatments available for human use, and testing of candidates requires the use of well-characterized animal models that mimic human disease. We performed a comprehensive and statistically-powered evaluation of the African green monkey model to define parameters critical to disease progression and the extent to which they correlate with human disease. African green monkeys were inoculated by the intratracheal route with 2.5 × 10(4 plaque forming units of the Malaysia strain of Nipah virus. Physiological data captured using telemetry implants and assessed in conjunction with clinical pathology were consistent with shock, and histopathology confirmed widespread tissue involvement associated with systemic vasculitis in animals that succumbed to acute disease. In addition, relapse encephalitis was identified in 100% of animals that survived beyond the acute disease phase. Our data suggest that disease progression in the African green monkey is comparable to the variable outcome of Nipah virus infection in humans.

  19. Hendra and Nipah viruses: pathogenesis, animal models and recent breakthroughs in vaccination

    Directory of Open Access Journals (Sweden)

    Weingartl HM

    2015-09-01

    Full Text Available Hana M Weingartl National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB, Canada Abstract: Hendra and Nipah viruses are two highly pathogenic zoonotic members of the genus Henipavirus, family Paramyxoviridae, requiring work under biosafety level 4 conditions due to a lack of effective therapy and human vaccines. Several vaccine candidates were protective in animal models: recombinant vaccinia virus expressing Nipah virus (NiV F and G proteins in hamsters against NiV; recombinant ALVAC–NiV F and G in swine against NiV; recombinant Hendra virus (HeV soluble G protein (sGHeV against HeV and NiV in cats, ferrets, horses, and African green monkeys (AGM; recombinant vesicular stomatitis virus-based vectors expressing NiV F or G against NiV in hamsters and ferrets; measles virus-based NiV G vaccine candidate in hamsters and AGMs against NiV; and adenoassociated virus expressing NiG protein, which protected hamsters against NiV. The sGHeV was licensed for use in horses (Equivac HeV® in 2012. It is the first vaccine candidate licensed against a biosafety level 4 agent. With the development of suitable animal models (ferret, hamster and, importantly, AGM, progress can be made toward development of a human vaccine.Keywords: henipavirus, equine, swine, human infection, animal models, vaccine candidates

  20. Chikungunya, Influenza, Nipah, and Semliki Forest Chimeric Viruses with Vesicular Stomatitis Virus: Actions in the Brain.

    Science.gov (United States)

    van den Pol, Anthony N; Mao, Guochao; Chattopadhyay, Anasuya; Rose, John K; Davis, John N

    2017-03-15

    Recombinant vesicular stomatitis virus (VSV)-based chimeric viruses that include genes from other viruses show promise as vaccines and oncolytic viruses. However, the critical safety concern is the neurotropic nature conveyed by the VSV glycoprotein. VSVs that include the VSV glycoprotein (G) gene, even in most recombinant attenuated strains, can still show substantial adverse or lethal actions in the brain. Here, we test 4 chimeric viruses in the brain, including those in which glycoprotein genes from Nipah, chikungunya (CHIKV), and influenza H5N1 viruses were substituted for the VSV glycoprotein gene. We also test a virus-like vesicle (VLV) in which the VSV glycoprotein gene is expressed from a replicon encoding the nonstructural proteins of Semliki Forest virus. VSVΔG-CHIKV, VSVΔG-H5N1, and VLV were all safe in the adult mouse brain, as were VSVΔG viruses expressing either the Nipah F or G glycoprotein. In contrast, a complementing pair of VSVΔG viruses expressing Nipah G and F glycoproteins were lethal within the brain within a surprisingly short time frame of 2 days. Intranasal inoculation in postnatal day 14 mice with VSVΔG-CHIKV or VLV evoked no adverse response, whereas VSVΔG-H5N1 by this route was lethal in most mice. A key immune mechanism underlying the safety of VSVΔG-CHIKV, VSVΔG-H5N1, and VLV in the adult brain was the type I interferon response; all three viruses were lethal in the brains of adult mice lacking the interferon receptor, suggesting that the viruses can infect and replicate and spread in brain cells if not blocked by interferon-stimulated genes within the brain.IMPORTANCE Vesicular stomatitis virus (VSV) shows considerable promise both as a vaccine vector and as an oncolytic virus. The greatest limitation of VSV is that it is highly neurotropic and can be lethal within the brain. The neurotropism can be mostly attributed to the VSV G glycoprotein. Here, we test 4 chimeric viruses of VSV with glycoprotein genes from Nipah

  1. Human metapneumovirus and respiratory syncytial virus in hospitalized danish children with acute respiratory tract infection

    DEFF Research Database (Denmark)

    von Linstow, Marie-Louise; Larsen, Hans Henrik; Eugen-Olsen, Jesper;

    2004-01-01

    The newly discovered human metapneumovirus (hMPV) has been shown to be associated with respiratory illness. We determined the frequencies and clinical features of hMPV and respiratory syncytial virus (RSV) infections in 374 Danish children with 383 episodes of acute respiratory tract infection...... children 1-6 months of age. Asthmatic bronchitis was diagnosed in 66.7% of hMPV and 10.6% of RSV-infected children (p respiratory support. hMPV is present in young...

  2. Production of monoclonal antibodies for Avian Metapneumovirus (SHS-BR-121 isolated in Brazil

    Directory of Open Access Journals (Sweden)

    LT Coswig

    2007-12-01

    Full Text Available Avian Metapneumovirus (aMPV, also called Turkey Rhinotracheitis Virus (TRTV, is an upper respiratory tract infection of turkeys, chickens and other avian species. Five monoclonal antibodies (MAbs were created against the Brazilian isolate (SHS-BR-121 of aMPV, MAbs 1A5B8; 1C1C4; 2C2E9 and 2A4C3 of IgG1 and MAb 1C1F8 of IgG2a. Four Mabs (1A5B8; 1C1C4; 2C2E9 and 2A4C3 showed neutralizing activity and three (1A5B8; 1C1C4 and 2A4C3 inhibited cellular fusion in vitro. These MAbs were used to investigate antigenic relationship among three strains (SHS-BR-121, STG 854/88 and TRT 1439/91 of aMPV subtypes A and B using cross-neutralization test. The results confirm that the monoclonal antibodies described can be used as a valuable tool in the epizootiological and serological studies, and also for the specific diagnosis of the subtypes in the infection for Avian Metapneumovirus.

  3. Genetic diversity and evolution of human metapneumovirus fusion protein over twenty years

    Directory of Open Access Journals (Sweden)

    Liem Alexis

    2009-09-01

    Full Text Available Abstract Background Human metapneumovirus (HMPV is an important cause of acute respiratory illness in children. We examined the diversity and molecular evolution of HMPV using 85 full-length F (fusion gene sequences collected over a 20-year period. Results The F gene sequences fell into two major groups, each with two subgroups, which exhibited a mean of 96% identity by predicted amino acid sequences. Amino acid identity within and between subgroups was higher than nucleotide identity, suggesting structural or functional constraints on F protein diversity. There was minimal progressive drift over time, and the genetic lineages were stable over the 20-year period. Several canonical amino acid differences discriminated between major subgroups, and polymorphic variations tended to cluster in discrete regions. The estimated rate of mutation was 7.12 × 10-4 substitutions/site/year and the estimated time to most recent common HMPV ancestor was 97 years (95% likelihood range 66-194 years. Analysis suggested that HMPV diverged from avian metapneumovirus type C (AMPV-C 269 years ago (95% likelihood range 106-382 years. Conclusion HMPV F protein remains conserved over decades. HMPV appears to have diverged from AMPV-C fairly recently.

  4. Excessive production and extreme editing of human metapneumovirus defective interfering RNA is associated with type I IFN induction

    NARCIS (Netherlands)

    B.G. van den Hoogen (Bernadette); S. van Boheemen (Sander); J.C.W.M. de Rijck (Jonneke); S. van Nieuwkoop (Stefan); D.J. Smith (Derek James); B.M. Laksono (Brigitta); A.P. Gultyaev (Alexander); A.D.M.E. Osterhaus (Albert); R.A.M. Fouchier (Ron)

    2014-01-01

    textabstractType I IFN production is one of the hallmarks of host innate immune responses upon virus infection. Whilst most respiratory viruses carry IFN antagonists, reports on human metapneumovirus (HMPV) have been conflicting. Using deep sequencing, we have demonstrated that HMPV particles accumu

  5. Mutation of domain III and domain VI in L gene conserved domain of Nipah virus

    Science.gov (United States)

    Jalani, Siti Aishah; Ibrahim, Nazlina

    2016-11-01

    Nipah virus (NiV) is the etiologic agent responsible for the respiratory illness and causes fatal encephalitis in human. NiV L protein subunit is thought to be responsible for the majority of enzymatic activities involved in viral transcription and replication. The L protein which is the viral RNA dependent RNA polymerase has high sequence homology among negative sense RNA viruses. In negative stranded RNA viruses, based on sequence alignment six conserved domain (domain I-IV) have been determined. Each domain is separated on variable regions that suggest the structure to consist concatenated functional domain. To directly address the roles of domains III and VI, site-directed mutations were constructed by the substitution of bases at sequences 2497, 2500, 5528 and 5532. Each mutated L gene can be used in future studies to test the ability for expression on in vitro translation.

  6. A wild goose metapneumovirus containing a large attachment glycoprotein is avirulent but immunoprotective in domestic turkeys.

    Science.gov (United States)

    Bennett, Richard S; LaRue, Rebecca; Shaw, Daniel; Yu, Qingzhong; Nagaraja, K V; Halvorson, David A; Njenga, M Kariuki

    2005-12-01

    The genomic structure and composition of an avian metapneumovirus (aMPV) recently isolated from wild Canada geese (goose 15a/01) in the United States, together with its replication, virulence, and immunogenicity in domestic turkeys, were investigated. The sizes of seven of the eight genes, sequence identity, and genome organization of goose aMPV were similar to those of turkey aMPV subtype C (aMPV/C) strains, indicating that it belonged to the subtype. However, the goose virus contained the largest attachment (G) gene of any pneumovirus or metapneumovirus, with the predicted G protein of 585 amino acids (aa) more than twice the sizes of G proteins from other subtype C viruses and human metapneumovirus and more than 170 aa larger than the G proteins from the other aMPV subtypes (subtypes A, B, and D). The large G gene resulted from a 1,015-nucleotide insertion at 18 nucleotides upstream of the termination signal of the turkey aMPV/C G gene. Three other aMPV isolates from Canada geese had similarly large G genes, whereas analysis of recent aMPV strains circulating in U.S. turkeys did not indicate the presence of the goose virus-like strain. In vitro, the goose virus replicated to levels (2 x 10(5) to 5 x 10(5) 50% tissue culture infective dose) comparable to those produced by turkey aMPV/C strains. More importantly, the virus replicated efficiently in the upper respiratory tract of domestic turkeys but with no clinical signs in either day-old or 2-week-old turkeys. The virus was also horizontally transmitted to naïve birds, and turkey infections with goose 15a/01 induced production of aMPV-specific antibodies. Challenging day-old or 2-week-old turkeys vaccinated with live goose aMPV resulted in lower clinical scores in 33% of the birds, whereas the rest of the birds had no detectable clinical signs of the upper respiratory disease, suggesting that the mutant virus may be a safe and effective vaccine against aMPV infection outbreaks in commercial turkeys.

  7. Differential Mucin Expression by Respiratory Syncytial Virus and Human Metapneumovirus Infection in Human Epithelial Cells

    Directory of Open Access Journals (Sweden)

    Ma. Del Rocío Baños-Lara

    2015-01-01

    Full Text Available Mucins (MUC constitute an important component of the inflammatory and innate immune response. However, the expression of these molecules by respiratory viral infections is still largely unknown. Respiratory syncytial virus (RSV and human metapneumovirus (hMPV are two close-related paramyxoviruses that can cause severe low respiratory tract disease in infants and young children worldwide. Currently, there is not vaccine available for neither virus. In this work, we explored the differential expression of MUC by RSV and hMPV in human epithelial cells. Our data indicate that the MUC expression by RSV and hMPV differs significantly, as we observed a stronger induction of MUC8, MUC15, MUC20, MUC21, and MUC22 by RSV infection while the expression of MUC1, MUC2, and MUC5B was dominated by the infection with hMPV. These results may contribute to the different immune response induced by these two respiratory viruses.

  8. Excretion patterns of human metapneumovirus and respiratory syncytial virus among young children

    DEFF Research Database (Denmark)

    von Linstow, M-L; Eugen-Olsen, J; Koch, A;

    2006-01-01

    BACKGROUND: As respiratory syncytial virus (RSV) and human metapneumovirus (hMPV) cause serious respiratory tract infections, the routes of transmission of these viruses are important to elucidate. We examined the modes of virus shedding and shedding duration of RSV and hMPV in young children...... of the five children shedding RNA in faeces had diarrhoea and children shedding RNA in sweat were either less than five weeks of age or had a chronic lung disease. RSV and hMPV RNA was shed in nasal secretions for a median of 11.5 and 5.0 days respectively (p = 0.001). More than 75% of the family members...... of the infected children showed to have an upper respiratory tract infection when following up. CONCLUSION: Viral RNA was present in nasal secretions, saliva, sweat, and faeces, but whether or not the virions were infectious and constitute a potential mode of transmission remains to be shown in future studies....

  9. Genotypes and clinical data of respiratory syncytial virus and metapneumovirus in brazilian infants: a new perspective

    Directory of Open Access Journals (Sweden)

    Adriana Gut Lopes Riccetto

    2009-02-01

    Full Text Available The aim of this study was to determine if there was a correlation between respiratory syncytial virus (RSV and metapneumovirus (MPV genotypes and clinical data of Brazilian infants hospitalized for acute lower respiratory infection. The viruses in the patients' nasopharyngeal secretions were studied using the polymerase chain reaction and phylogenetic analysis. The study assessed 144 infants; 31.9% were RSV positive and 5.6% were MPV positive. Statistical analysis was performed using the chi-squared test, Fisher's test, Odds ratio, univariate logistic regression, non-conditional multivariate logistic regression and the forward - stepwise method. Multivariate analysis confirmed a significant relationship between a positive PCR test for RSV and hospitalization during the month of May and with pulse oximetry less than 90%. The phylogenetic analysis indicated the genotypes GA2, GA5, SAA1 (Group A, SAB1, SAB3 and BA (Group B for RSV and Group B, subgroup B1, for MPV.

  10. METAPNEUMOVIRUS AND BOKAVIRUS RESPIRATORY INFECTIONS IN THE STRUCTURE OF SARS IN CHILDREN

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    F. S. Harlamov

    2015-01-01

    Full Text Available The article provides an overview of the literature data about new pneumotropic viruses - metapneumovirus and bokavirus: taxonomy, structural features, pathogenesis, laboratory diagnosis, clinical symptoms of the diseases they cause and complications. The high incidence and bokavirus metapnevmovirus structure of SARS infections in preschool children, the authors have shown the example carried out at the Department of Infectious Diseases in Children Medical University (now RNIMU named after N.I. Pirogov and on the basis of clinical Institute of Virology, multicenter, randomized, blind, placebo-controlled study on the therapeutic efficacy and safety of interferon inducer Kagocel in 120 children aged 2 to 6 years. The findings to point out on significant reduction in the rate of relief of basic clinical manifestations of SARS, regardless of etiology, in children taking Kagocel in compare with a group of children who took a placebo.

  11. Comparison of severe pneumonia caused by Human metapneumovirus and respiratory syncytial virus in hospitalized children

    Directory of Open Access Journals (Sweden)

    Yuqing Wang

    2014-01-01

    Full Text Available Objectives: The objective of this study is to compare the incidence and clinical characteristics of severe pneumonia caused by Human metapneumovirus (hMPV to respiratory syncytial virus (RSV infection in children. Patients and Methods: A total of 151 children hospitalized with severe pneumonia, were tested for hMPV using reverse-transcription polymerase chain reaction. At the same time, samples were tested for RSV and other common respiratory viruses. Medical records, including clinical, laboratory data, and chest radiography findings, were reviewed for all children. Results: Of the 151 samples, 88 (58.3% were positive for respiratory viruses. Of the 88 positive, there were 6 (4.0% with hMPV, 66 (43.7% with RSV, 13 (8.6% with influenza A, 2 (1.3% with parainfluenza virus III, 1 (0.7% with parainfluenza virus I, 1 (0.7% with adenovirus and 1 (0.7% with influenza B. hMPV-infected patients were significantly older than RSV-infected patients (P < 0.001. Children with hMPV pneumonia had fever more frequently (P = 0.03. Two hMPV-positive patients (33.3% required admission to an intensive care unit, and two patients (33.3% required mechanical ventilation. The duration of illness was 18.33 ± 7.09 days. These characteristics of hMPV infections were similar to patients with RSV infections. Conclusion: Human metapneumovirus is an infrequent viral pathogen causing severe pneumonia in children. Children with hMPV were older than those with RSV. The disease caused by hMPV was similar in presentation and severity to RSV, with a minority of children requiring additional respiratory support.

  12. Respiratory Failure Secondary to Human Metapneumovirus Requiring Extracorporeal Membrane Oxygenation in a 32-Month-Old Child

    Directory of Open Access Journals (Sweden)

    Abha Gupta

    2012-01-01

    Full Text Available Human metapneumovirus (HMPV is a common virus that can cause respiratory problems ranging from mild upper respiratory tract disease to respiratory failure requiring mechanical support. Here, we report a case of a 32-month-old male with a previous history of asthma, who developed respiratory failure two weeks after onset of cough and rhinorrhea and required extracorporeal membrane oxygenation (ECMO for 9 days after failing high-frequency oscillatory ventilation (HFOV. To our knowledge, this is the oldest reported pediatric patient with respiratory failure secondary to human metapneumovirus that did not respond to mechanical ventilation. This case highlights three critical points: the potentially fatal causative role of HMPV in respiratory failure in an older pediatric age group of immunocompetent hosts, the importance of early recognition of impending respiratory failure, and the timely utilization of ECMO.

  13. The Nature of Exposure Drives Transmission of Nipah Viruses from Malaysia and Bangladesh in Ferrets

    Science.gov (United States)

    Clayton, Bronwyn A.; Middleton, Deborah; Arkinstall, Rachel; Frazer, Leah; Wang, Lin-Fa; Marsh, Glenn A.

    2016-01-01

    Person-to-person transmission is a key feature of human Nipah virus outbreaks in Bangladesh. In contrast, in an outbreak of Nipah virus in Malaysia, people acquired infections from pigs. It is not known whether this important epidemiological difference is driven primarily by differences between NiV Bangladesh (NiV-BD) and Malaysia (NiV-MY) at a virus level, or by environmental or host factors. In a time course study, ferrets were oronasally exposed to equivalent doses of NiV-BD or NiV-MY. More rapid onset of productive infection and higher levels of virus replication in respiratory tract tissues were seen for NiV-BD compared to NiV-MY, corroborating our previous report of increased oral shedding of NiV-BD in ferrets and suggesting a contributory mechanism for increased NiV-BD transmission between people compared to NiV-MY. However, we recognize that transmission occurs within a social and environmental framework that may have an important and differentiating role in NiV transmission rates. With this in mind, ferret-to-ferret transmission of NiV-BD and NiV-MY was assessed under differing viral exposure conditions. Transmission was not identified for either virus when naïve ferrets were cohoused with experimentally-infected animals. In contrast, all naïve ferrets developed acute infection following assisted and direct exposure to oronasal fluid from animals that were shedding either NiV-BD or NiV-MY. Our findings for ferrets indicate that, although NiV-BD may be shed at higher levels than NiV-MY, transmission risk may be equivalently low under exposure conditions provided by cohabitation alone. In contrast, active transfer of infected bodily fluids consistently results in transmission, regardless of the virus strain. These observations suggest that the risk of NiV transmission is underpinned by social and environmental factors, and will have practical implications for managing transmission risk during outbreaks of human disease. PMID:27341030

  14. Metapneumovirus humano (hMPV asociado con exacerbación de asma aguda bronquial severa Human Metapneumovirus (hMPV associated to severe bronchial asthmatic crisis

    Directory of Open Access Journals (Sweden)

    M. A. López

    2006-09-01

    Full Text Available El metapneumovirus humano (hMPV es un nuevo agente causal de infección aguda del tracto respiratorio, recientemente reportado tras su hallazgo en niños, jóvenes, adultos y ancianos. Las manifestaciones clínicas producidas por el hMPV son indistinguibles de aquellas provocadas por los virus respiratorios clásicamente conocidos, y varían desde infección asintomática hasta neumonía complicada. Por otro lado, se han descrito casos de exacerbación de asma bronquial asociados a la infección con hMPV. En este trabajo se describe el caso de un niño hospitalizado que presentó una crisis asmática bronquial severa con sospecha de una infección viral asociada. Por el test de inmunofluorescencia indirecta no se detectaron virus sincicial respiratorio (VSR, adenovirus, virus influenza a - b ni virus parainfluenza 1, 2 y 3. En un intento por detectar la presencia de hMPV, se realizó una RT-PCR para la amplificación de los genes N y F con resultado positivo. Conforme a nuestro conocimiento, esta sería la primera descripción de un caso de exacerbación de asma asociado a hMPV en nuestra región. Los resultados de este estudio serían similares a los reportados por otros autores, quienes postulan que, a semejanza de lo que ocurre con el VSR, una infección por hMPV puede gatillar una enfermedad respiratoria crónica, como el asma.Human Metapneumovirus (hMPV is a recently reported agent of acute infection in the respiratory tract. It has been found in children as well as in young adults and elders. The clinical manifestations produced by hMPV are indistinguishable from those by common respiratory virus, and can evolve from asymptomatic infection into severe pneumonia. On the other hand, some authors have described cases of bronchial asthma exacerbation associated with hMPV infection. In this work we report a case of a child who presented a severe bronchial asthmatic crisis with a suspected viral associated infection. Immunofluorescence tests

  15. Limited inter- and intra-patient sequence diversity of the genetic lineage A human metapneumovirus fusion gene

    DEFF Research Database (Denmark)

    Winther, Thilde Nordmann; Madsen, Chris D; Pedersen, Anders

    2005-01-01

    Human metapneumovirus (hMPV) is associated with respiratory tract illness especially in young children. Two hMPV genetic lineages, A and B, and four sublineages A1, A2 and B1, B2 have been defined. Infection with hMPV occurs through membrane fusion mediated by the hMPV fusion (F) protein. In this...... diversity observed lay emphasis on the hMPV F gene as a putative target for future vaccine development....

  16. Expression and purification of the matrix protein of Nipah virus in baculovirus insect cell system.

    Science.gov (United States)

    Masoomi Dezfooli, Seyedehsara; Tan, Wen Siang; Tey, Beng Ti; Ooi, Chien Wei; Hussain, Siti Aslina

    2016-01-01

    Nipah virus (NiV) causes fatal respiratory illness and encephalitis in humans and animals. The matrix (M) protein of NiV plays an important role in the viral assembly and budding process. Thus, an access to the NiV M protein is vital to the design of viral antigens as diagnostic reagents. In this study, recombinant DNA technology was successfully adopted in the cloning and expression of NiV M protein. A recombinant expression cassette (baculovirus expression vector) was used to encode an N-terminally His-tagged NiV M protein in insect cells. A time-course study demonstrated that the highest yield of recombinant M protein (400-500 μg) was expressed from 107 infected cells 3 days after infection. A single-step purification method based on metal ion affinity chromatography was established to purify the NiV M protein, which successfully yielded a purity level of 95.67% and a purification factor of 3.39. The Western blotting and enzyme-linked immunosorbent assay (ELISA) showed that the purified recombinant M protein (48 kDa) was antigenic and reacted strongly with the serum of a NiV infected pig.

  17. Development of a neutralization assay for Nipah virus using pseudotype particles.

    Science.gov (United States)

    Tamin, Azaibi; Harcourt, Brian H; Lo, Michael K; Roth, James A; Wolf, Mike C; Lee, Benhur; Weingartl, Hana; Audonnet, Jean-Christophe; Bellini, William J; Rota, Paul A

    2009-09-01

    Nipah virus (NiV) and Hendra virus (HeV) are zoonotic paramyxoviruses capable of causing severe disease in humans and animals. These viruses require biosafety level 4 (BSL-4) containment. Like other paramyxoviruses, the plaque reduction neutralization test (PRNT) can be used to detect antibodies to the surface glycoproteins, fusion (F) and attachment (G), and PRNT titers give an indication of protective immunity. Unfortunately, for NiV and HeV, the PRNT must be performed in BSL-4 containment and takes several days to complete. Thus, we have developed a neutralization assay using VSV pseudotype particles expressing the F and G proteins of NiV (pVSV-NiV-F/G) as target antigens. This rapid assay, which can be performed at BSL-2, was evaluated using serum samples from outbreak investigations and more than 300 serum samples from an experimental NiV vaccination study in swine. The results of the neutralization assays with pVSV-NiV-F/G as antigen showed a good correlation with those of standard PRNT. Therefore, this new method has the potential to be a rapid and cost-effective diagnostic method, especially in locations that lack high containment facilities, and will provide a valuable tool for basic research and vaccine development.

  18. Region of Nipah virus C protein responsible for shuttling between the cytoplasm and nucleus.

    Science.gov (United States)

    Horie, Ryo; Yoneda, Misako; Uchida, Shotaro; Sato, Hiroki; Kai, Chieko

    2016-10-01

    Nipah virus (NiV) causes severe encephalitis in humans, with high mortality. NiV nonstructural C protein (NiV-C) is essential for its pathogenicity, but its functions are unclear. In this study, we focused on NiV-C trafficking in cells and found that it localizes predominantly in the cytoplasm but partly in the nucleus. An analysis of NiV-C mutants showed that amino acids 2, 21-24 and 110-139 of NiV-C are important for its localization in the cytoplasm. Inhibitor treatment indicates that the nuclear export determinant is not a classical CRM1-dependent nuclear export signal. We also determined that amino acids 60-75 and 72-75 were important for nuclear localization of NiV-C. Furthermore, NiV-C mutants that had lost their capacity for nuclear localization inhibited the interferon (IFN) response more strongly than complete NiV-C. These results indicate that the IFN-antagonist activity of NiV-C occurs in the cytoplasm.

  19. Nipah disease, a new zoonosis%一种新的人畜共患病--尼帕病

    Institute of Scientific and Technical Information of China (English)

    何成伟

    2001-01-01

    @@ 尼帕病(Nipah,有人翻译为宜麦病)是1998年在马来西亚首次发现的鲜为人知的一种人畜共患传染病,严重危害人和猪,发病急,致死率高.其特征性症状是严重的呼吸道和神经症状,患者颈部和腹部痉挛,公猪和母猪则表现为突然死亡[1].

  20. Molecular detection of infectious bronchitis and avian metapneumoviruses in Oman backyard poultry.

    Science.gov (United States)

    Al-Shekaili, Thunai; Baylis, Matthew; Ganapathy, Kannan

    2015-04-01

    Infectious bronchitis virus (IBV) and avian metapneumovirus (aMPV) are economically important viral pathogens infecting chickens globally. Identification of endemic IBV and aMPV strains promotes better control of both diseases and prevents production losses. Orophrayngeal swab samples were taken from 2317 birds within 243 different backyard flocks in Oman. Swabs from each flock were examined by RT-PCR using part-S1 and G gene primers for IBV and aMPV respectively. Thirty-nine chicken flocks were positive for IBV. Thirty two of these were genotyped and they were closely related to 793/B, M41, D274, IS/1494/06 and IS/885/00. 793/B-like IBV was also found in one turkey and one duck flock. Five flocks were positive for aMPV subtype B. Though no disease was witnessed at the time of sampling, identified viruses including variant IBV strains, may still pose a threat for both backyard and commercial poultry in Oman. Copyright © 2014 Elsevier Ltd. All rights reserved.

  1. New-Onset Myocarditis in an Immunocompetent Adult with Acute Metapneumovirus Infection.

    Science.gov (United States)

    Weinreich, Mark A; Jabbar, Ahmad Y; Malguria, Nagina; Haley, Robert W

    2015-01-01

    Introduction. A number of viruses have been implicated in viral myocarditis; however, there has been no previous report of human metapneumovirus (hMPV) causing this condition. Discovered in 2001, hMPV is typically associated with upper respiratory illness, mainly affecting children. Case Presentation. We report the case of a 25-year-old man with acute systolic heart failure from viral myocarditis secondary to the hMPV. The patient was initially admitted to the general medical ward but developed increasing oxygen requirements resulting in transfer to the cardiac intensive care unit. Cardiac magnetic resonance imaging was used to help confirm the diagnosis. He was treated with intravenous diuretics, and afterload and preload agents, and he was subsequently discharged home after seven days of hospitalization. Discussion. hMPV is typically a respiratory pathogen; however, it was associated with in myocarditis in our patient. Due to the recent ability to detect this virus, we may see more cases of this, particularly during peak months of infection. Conclusion. This is the first case description of myocarditis associated with hMPV infection.

  2. New-Onset Myocarditis in an Immunocompetent Adult with Acute Metapneumovirus Infection

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    Mark A. Weinreich

    2015-01-01

    Full Text Available Introduction. A number of viruses have been implicated in viral myocarditis; however, there has been no previous report of human metapneumovirus (hMPV causing this condition. Discovered in 2001, hMPV is typically associated with upper respiratory illness, mainly affecting children. Case Presentation. We report the case of a 25-year-old man with acute systolic heart failure from viral myocarditis secondary to the hMPV. The patient was initially admitted to the general medical ward but developed increasing oxygen requirements resulting in transfer to the cardiac intensive care unit. Cardiac magnetic resonance imaging was used to help confirm the diagnosis. He was treated with intravenous diuretics, and afterload and preload agents, and he was subsequently discharged home after seven days of hospitalization. Discussion. hMPV is typically a respiratory pathogen; however, it was associated with in myocarditis in our patient. Due to the recent ability to detect this virus, we may see more cases of this, particularly during peak months of infection. Conclusion. This is the first case description of myocarditis associated with hMPV infection.

  3. The pathogenicity of avian metapneumovirus subtype C wild bird isolates in domestic turkeys

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    Cha Ra Mi

    2013-01-01

    Full Text Available Abstract Background Avian metapneumovirus subtype C (aMPV/C causes severe upper respiratory disease in turkeys. Previous report revealed the presence of aMPV/C in wild birds in the southeast regions of the U.S. Methods In this study, aMPV/C positive oral swabs from American coots (AC and Canada geese (CG were passaged three times in the respiratory tract of specific pathogen free (SPF turkeys and used as aMPV/C P3 virus isolates in subsequent studies. Results Wild bird P3 isolates showed similar growth characteristics when compared to virulent aMPV/C in chicken embryo fibroblast ( CEF cell cultures and their glycoprotein G gene sequence was closely related to the G gene of aMPV/C Colorado reference virus. Three-day-old commercial or SPF turkeys were inoculated oculonasally with wild bird aMPV/C P3 isolates. At 5 and 7 days post-inoculation (DPI, severe clinical signs were observed in both of the AC and CG virus-exposed groups. Viral RNA was detected in tracheal swabs by reverse transcriptase polymerase chain reaction (RT-PCR. In addition, immunohistochemistry showed virus replication in the nasal turbinate and trachea. All virus-exposed turkeys developed positive antibody response by 14 DPI. Conclusions Our data demonstrate that aMPV/C wild bird isolates induced typical aMPV/C disease in the domestic turkeys.

  4. Avian metapneumovirus excretion in vaccinated and non-vaccinated specified pathogen free laying chickens.

    Science.gov (United States)

    Hess, M; Huggins, M B; Mudzamiri, R; Heincz, U

    2004-02-01

    Vaccinated and non-vaccinated specified pathogen-free White Leghorn laying chickens were challenged at peak of lay by the intravenous or oculonasal route with a virulent avian metapneumovirus (aMPV) subtype B chicken strain. Severe clinical signs and a drop in egg production were induced in the non-vaccinated intravenously challenged birds whereas the vaccinates were not affected. Live virus excretion was demonstrated in the faeces and respiratory tract of non-vaccinated hens for up to 7 days post intravenous challenge. After oculonasal challenge, virus excretion could only be demonstrated in the respiratory tract for up to 5 days. No live virus excretion was found in either the faeces or the respiratory tract of vaccinated birds. Concurrent with live virus isolation, the presence of viral RNA was demonstrated by single reverse transcription-polymerase chain reaction (RT-PCR). Nested RT-PCR was more sensitive and viral RNA could be detected in non-vaccinated birds up to 28 days post either intravenous or oculonasal challenge, at which time the experiment was terminated. Viral RNA was detected for up to 12 days in vaccinated birds. This is the first study investigating excretion of aMPV and viral RNA in vaccinated and non-vaccinated laying hens challenged under experimental conditions. The results are of importance with regard to the persistence of aMPV and the appropriate diagnostic detection method in laying birds.

  5. Avian metapneumovirus subgroup C induces autophagy through the ATF6 UPR pathway.

    Science.gov (United States)

    Hou, Lei; Wei, Li; Zhu, Shanshan; Wang, Jing; Quan, Rong; Li, Zixuan; Liu, Jue

    2017-09-26

    An increasing number of studies have demonstrated that macroautophagy/autophagy plays an important role in the infectious processes of diverse pathogens. However, it remains unknown whether autophagy is induced in avian metapneumovirus (aMPV)-infected host cells, and, if so, how this occurs. Here, we report that aMPV subgroup C (aMPV/C) induces autophagy in cultured cells. We demonstrated this relationship by detecting classical autophagic features, including the formation of autophagsomes, the presence of GFP-LC3 puncta and the conversation of LC3-I into LC3-II. Also, we used pharmacological regulators and siRNAs targeting ATG7 or LC3 to examine the role of autophagy in aMPV/C replication. The results showed that autophagy is required for efficient replication of aMPV/C. Moreover, infection with aMPV/C promotes autophagosome maturation and induces a complete autophagic process. Finally, the ATF6 pathway, of which one component is the unfolded protein response (UPR), becomes activated in aMPV/C-infected cells. Knockdown of ATF6 inhibited aMPV/C-induced autophagy and viral replication. Collectively, these results not only show that autophagy promotes aMPV/C replication in the cultured cells, but also reveal that the molecular mechanisms underlying aMPV/C-induced autophagy depends on regulation of the ER stress-related UPR pathway.

  6. Brazilian avian metapneumovirus subtypes A and B: experimental infection of broilers and evaluation of vaccine efficacy

    Directory of Open Access Journals (Sweden)

    Márcia B. dos Santos

    2012-12-01

    Full Text Available Avian metapneumovirus (aMPV is a respiratory pathogen associated with the swollen head syndrome (SHS in chickens. In Brazil, live aMPV vaccines are currently used, but subtypes A and, mainly subtype B (aMPV/A and aMPV/B are still circulating. This study was conducted to characterize two Brazilian aMPV isolates (A and B subtypes of chicken origin. A challenge trial to explore the replication ability of the Brazilian subtypes A and B in chickens was performed. Subsequently, virological protection provided from an aMPV/B vaccine against the same isolates was analyzed. Upon challenge experiment, it was shown by virus isolation and real time PCR that aMPV/B could be detected longer and in higher amounts than aMPV/A. For the protection study, 18 one-day-old chicks were vaccinated and challenged at 21 days of age. Using virus isolation and real time PCR, no aMPV/A was detected in the vaccinated chickens, whereas one vaccinated chicken challenged with the aMPV/B isolate was positive. The results showed that aMPV/B vaccine provided a complete heterologous virological protection, although homologous protection was not complete in one chicken. Although only one aMPV/B positive chicken was detected after homologous vaccination, replication in vaccinated animals might allow the emergence of escape mutants.

  7. Human Metapneumovirus Is Capable of Entering Cells by Fusion with Endosomal Membranes.

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    Reagan G Cox

    2015-12-01

    Full Text Available Human metapneumovirus (HMPV, a member of the Paramyxoviridae family, is a leading cause of lower respiratory illness. Although receptor binding is thought to initiate fusion at the plasma membrane for paramyxoviruses, the entry mechanism for HMPV is largely uncharacterized. Here we sought to determine whether HMPV initiates fusion at the plasma membrane or following internalization. To study the HMPV entry process in human bronchial epithelial (BEAS-2B cells, we used fluorescence microscopy, an R18-dequenching fusion assay, and developed a quantitative, fluorescence microscopy assay to follow virus binding, internalization, membrane fusion, and visualize the cellular site of HMPV fusion. We found that HMPV particles are internalized into human bronchial epithelial cells before fusing with endosomes. Using chemical inhibitors and RNA interference, we determined that HMPV particles are internalized via clathrin-mediated endocytosis in a dynamin-dependent manner. HMPV fusion and productive infection are promoted by RGD-binding integrin engagement, internalization, actin polymerization, and dynamin. Further, HMPV fusion is pH-independent, although infection with rare strains is modestly inhibited by RNA interference or chemical inhibition of endosomal acidification. Thus, HMPV can enter via endocytosis, but the viral fusion machinery is not triggered by low pH. Together, our results indicate that HMPV is capable of entering host cells by multiple pathways, including membrane fusion from endosomal compartments.

  8. Genetic diversity and molecular evolution of the major human metapneumovirus surface glycoproteins over a decade.

    Science.gov (United States)

    Papenburg, Jesse; Carbonneau, Julie; Isabel, Sandra; Bergeron, Michel G; Williams, John V; De Serres, Gaston; Hamelin, Marie-Ève; Boivin, Guy

    2013-11-01

    Human metapneumovirus (HMPV) is a recently discovered paramyxovirus that is a major cause of respiratory infections worldwide. We aim to describe the molecular evolution of the HMPV F (fusion) and G (attachment) surface glycoproteins because they are targets for vaccines, monoclonal antibodies and antivirals currently in development. Nasopharyngeal aspirates were collected in children genetic lineages (A1, A2a, A2b, B1 and B2). Multiple lineages circulated each year in Quebec City. With the exception of B1, each of the 5 subgroups was the predominant lineage during ≥1 season. The A1 lineage was not detected since 2002-2003 in our local cohort. There was no evidence of inter- or intragenic recombination. HMPV-F was highly conserved, whereas HMPV-G exhibited greater diversity. HMPV-F demonstrated strong evidence of purifying selection, both overall and in an abundance of negatively selected amino acid sites. In contrast, sites under diversifying selection were detected in all HMPV-G lineages (range, 4-15), all of which were located in the ectodomain. Predominant circulating HMPV lineages vary by year. HMPV-F is highly constrained and undergoes significant purifying selection. Given its high genetic variability, we found a modest number of positively selected sites in HMPV-G. Copyright © 2013 Elsevier B.V. All rights reserved.

  9. Structure and self-assembly of the calcium binding matrix protein of human metapneumovirus.

    Science.gov (United States)

    Leyrat, Cedric; Renner, Max; Harlos, Karl; Huiskonen, Juha T; Grimes, Jonathan M

    2014-01-07

    The matrix protein (M) of paramyxoviruses plays a key role in determining virion morphology by directing viral assembly and budding. Here, we report the crystal structure of the human metapneumovirus M at 2.8 Å resolution in its native dimeric state. The structure reveals the presence of a high-affinity Ca²⁺ binding site. Molecular dynamics simulations (MDS) predict a secondary lower-affinity site that correlates well with data from fluorescence-based thermal shift assays. By combining small-angle X-ray scattering with MDS and ensemble analysis, we captured the structure and dynamics of M in solution. Our analysis reveals a large positively charged patch on the protein surface that is involved in membrane interaction. Structural analysis of DOPC-induced polymerization of M into helical filaments using electron microscopy leads to a model of M self-assembly. The conservation of the Ca²⁺ binding sites suggests a role for calcium in the replication and morphogenesis of pneumoviruses.

  10. Premature infants have impaired airway antiviral IFNγ responses to human metapneumovirus compared to respiratory syncytial virus

    Science.gov (United States)

    Pancham, Krishna; Perez, Geovanny F.; Huseni, Shehlanoor; Jain, Amisha; Kurdi, Bassem; Rodriguez-Martinez, Carlos E.; Preciado, Diego; Rose, Mary C.; Nino, Gustavo

    2017-01-01

    BACKGROUND It is unknown why human metapneumovirus (HMPV) and respiratory syncytial virus (RSV) cause severe respiratory infection in children, particularly in premature infants. Our aim was to investigate if there are defective airway antiviral responses to these viruses in young children with history of prematurity. METHODS Nasal airway secretions were collected from 140 children ≤3 y old without detectable virus (n = 80) or with PCR-confirmed HMPV or RSV infection (n = 60). Nasal protein levels of IFNγ, CCL5/RANTES, IL-10, IL-4, and IL-17 were determined using a multiplex magnetic bead immunoassay. RESULTS Full-term children with HMPV and RSV infection had increased levels of nasal airway IFNγ, CCL5, and IL-10 along with an elevation in Th1 (IFNγ)/Th2 (IL-4) ratios, which is expected during antiviral responses. In contrast, HMPV-infected premature children (< 32 wk gestation) did not exhibit increased Th1/Th2 ratios or elevated nasal airway secretion of IFNγ, CCL5, and IL-10 relative to uninfected controls. CONCLUSION Our study is the first to demonstrate that premature infants have defective IFNγ, CCL5/RANTES, and IL-10 airway responses during HMPV infection and provides novel insights about the potential reason why HMPV causes severe respiratory disease in children with history of prematurity. PMID:26086642

  11. Human Metapneumovirus Infection is Associated with Severe Respiratory Disease in Preschool Children with History of Prematurity

    Science.gov (United States)

    Pancham, Krishna; Sami, Iman; Perez, Geovanny F.; Huseni, Shehlanoor; Kurdi, Bassem; Rose, Mary C.; Rodriguez-Martinez, Carlos E.; Nino, Gustavo

    2017-01-01

    Rationale Human metapneumovirus (HMPV) is a recently discovered respiratory pathogen of the family Paramyxoviridae, the same of Respiratory Syncytial Virus (RSV). Premature children are at high risk of severe RSV infections, but it is unclear whether HMPV infection is more severe in hospitalized children with history of severe prematurity. Methods We conducted a retrospective analysis of the clinical respiratory presentation of all PCR-confirmed HMPV infections in preschool age children (≤5 yrs.) with and without history of severe prematurity (<32 weeks gestation). Respiratory distress scores were developed to examine the clinical severity of HMPV infections. Demographic and clinical variables were obtained from reviewing electronic medical records (EMR). Results A total of 571 pre-school children were identified by PCR-confirmed viral respiratory tract infection during the study period. HMPV was identified as a causative organism in 63 cases (11%). Fifty–eight (n=58) preschool age children with HMPV infection were included in this study after excluding those with significant co-morbidities. Our data demonstrated that 32.7% of children admitted with HMPV had history of severe prematurity. Preschool children with history of prematurity had more severe HMPV disease as illustrated by longer hospitalizations, new or increased need for supplemental O2, and higher severity scores independently of age, ethnicity and history of asthma. Conclusion Our study suggests that HMPV infection causes significant disease burden among preschool children with history of prematurity leading to severe respiratory infections and increasing health care resource utilization due to prolonged hospitalizations. PMID:26117550

  12. Small Animal Models for Human Metapneumovirus: Cotton Rat is More Permissive than Hamster and Mouse

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    Yu Zhang

    2014-07-01

    Full Text Available Human metapneumovirus (hMPV is the second most prevalent causative agent of pediatric respiratory infections worldwide. Currently, there are no vaccines or antiviral drugs against this virus. One of the major hurdles in hMPV research is the difficulty to identify a robust small animal model to accurately evaluate the efficacy and safety of vaccines and therapeutics. In this study, we compared the replication and pathogenesis of hMPV in BALB/c mice, Syrian golden hamsters, and cotton rats. It was found that BALB/c mice are not permissive for hMPV infection despite the use of a high dose (6.5 log10 PFU of virus for intranasal inoculation. In hamsters, hMPV replicated efficiently in nasal turbinates but demonstrated only limited replication in lungs. In cotton rats, hMPV replicated efficiently in both nasal turbinate and lung when intranasally administered with three different doses (4, 5, and 6 log10 PFU of hMPV. Lungs of cotton rats infected by hMPV developed interstitial pneumonia with mononuclear cells infiltrates and increased lumen exudation. By immunohistochemistry, viral antigens were detected at the luminal surfaces of the bronchial epithelial cells in lungs. Vaccination of cotton rats with hMPV completely protected upper and lower respiratory tract from wildtype challenge. The immunization also elicited elevated serum neutralizing antibody. Collectively, these results demonstrated that cotton rat is a robust small animal model for hMPV infection.

  13. Human metapneumovirus in patients hospitalized with acute respiratory infections: A meta-analysis.

    Science.gov (United States)

    Lefebvre, Annick; Manoha, Catherine; Bour, Jean-Baptiste; Abbas, Rachid; Fournel, Isabelle; Tiv, Michel; Pothier, Pierre; Astruc, Karine; Aho-Glélé, Ludwig Serge

    2016-08-01

    This meta-analysis aimed to estimate the prevalence of human metapneumovirus (hMPV) infections in patients hospitalized for acute respiratory infection (ARI) and to study factors associated with this prevalence. Medline and ScienceDirect databases were searched for prospective observational studies that screened hospitalized patients with ARI for hMPV by RT-PCR, with data available at December 27, 2014. The risk of bias was assessed regarding participation rate, definition of ARI, description of diagnostic technique, method of inclusion identical for all subjects, standardized and identical sampling method for all subjects, analysis performed according to the relevant subgroups, and presentation of data sources. Random-effect meta-analysis with arcsine transformation and meta-regressions was used. In the 75 articles included, the prevalence of hMPV among hospitalized ARI was 6.24% (95% CI 5.25-7.30). An effect of the duration of the inclusion period was observed (p=0.0114), with a higher prevalence of hMPV in studies conducted during periods of 7-11 months (10.56%, 95% CI 5.97-16.27) or complete years (7.55%, 95% CI 5.90-9.38) than in periods of 6 months or less (5.36%, 95% CI 4.29-6.54). A significant increase in the incidence with increasing distance from the equator was observed (p=0.0384). hMPV should be taken into account as a possible etiology in hospitalized ARI.

  14. High seroprevalence of human metapneumovirus infection in children in Chongqing,China

    Institute of Scientific and Technical Information of China (English)

    ZHANG Qin; YANG Xi-qiang; ZHAO Yao; ZHAO Xiao-dong

    2008-01-01

    Background The human metapneumovirus (hMPV) is a newly discovered respiratory viral pathogen that was first isolated in 2001 in the Netherlands.Its global distribution and long history of infection in humans have been well documented.In this study,we assessed the seropositivity of hMPV IgG antibodies in children in Chongqing,China.Methods The specificity of the enzyme linked immunosorbent assay (ELISA) was first validated by using respiratory syncytial virus (RSV) infected,antigen subtracted reference serum and by performing western blotting using anti-hMPV animal serum.This assay was used to determine the presence of the IgG antibody against hMPV and RSV in 325 serum samples obtained from children aged 0-6 years.Results No crossreaction was detected by ELISA between the antibodies to hMPV and RSV.The seropositivity of the anti-hMPV IgG antibody was 74.5% in children aged 0 to 5 months,64.0% in 6 to 11 months,72.7% in 12 to 23 months,87.1% in 24 to 35 months and 90.3% in 3 to 6 years.Conclusions hMPV is a common and significant respiratory pathogen in Chinese children.Almost all individuals are exposed to hMPV by age 6 years.

  15. Molecular Analysis of Human Metapneumovirus Detected in Patients with Lower Respiratory Tract Infection in Upper Egypt

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    Mona S. Embarek Mohamed

    2014-01-01

    Full Text Available Introduction. Since 2001, when Human metapneumovirus (HMPV was isolated in the Netherlands, the virus has been detected in several continents. Although reports have confirmed the prevalence of HMPV worldwide, data from Egypt remain limited. HMPV plays an important role in respiratory tract infections in individuals of all ages particularly in children. This study was aimed at estimating the prevalence of HMPV in patients with community-acquired lower respiratory infection in Upper Egypt and characterizing the circulating Egyptian HMPV strains for the first time. Materials and Methods. From 2005 to 2008, respiratory samples from 520 patients were analyzed for the presence of HMPV by real-time RT-PCR. Molecular and phylogenetic analyses were performed on partial fusion gene sequences of HMPV-positive patients. Results. HMPV-positive patients were detected in 2007-2008. The overall infection rate was 4%, while 57% of the patients were children. Sequence analysis demonstrated circulation of subgroup B viruses with predominance of lineage B2. Nucleotide sequence identity within lineage B1 was 98.8%–99.7% and higher than that in lineage B2 (94.3%–100%. Three new amino acid substitutions (T223N, R229K, and D280N of lineage B2 were observed. Conclusion. HMPV is a major viral pathogen in the Egyptian population especially in children. During 2007-2008, predominantly HMPV B2 circulated in Upper Egypt.

  16. Pengaruh Konsentrasi Asam Sitrat dan Suhu Pengempaan terhadap Kualitas Papan Partikel Pelepah Nipah

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    Ragil Widyorini

    2014-01-01

    Full Text Available Papan partikel tanpa perekat sintesis atau binderlessboard merupakan alternatif produk ramah lingkungan yang potensial dikembangkan di Indonesia. Kelemahan produk tersebut diantaranya adalah kestabilan dimensinya yang relatif rendah. Alternatif perbaikan produk bisa dilakukan dengan menambahkan bahan pengaktif komponen kimia. Asam sitrat memiliki tiga gugus karboksil dan diharapkan dapat membentuk ikatan ester dengan gugus hidroksil pada permukaan kayu. Penelitian menggunakan asam sitrat relatif baru dan belum banyak dikembangkan, oleh karena itu penelitian ini ditujukan untuk pengembangan produk biokomposit dengan menggunakan asam sitrat sebagai bahan pengikat. Bahan yang digunakan adalah pelepah nipah dengan ukuran partikel (halus dan kasar, konsentrasi asam sitrat (0% dan 10%, dan suhu pengempaan (180ºC dan 200°C. Pengujian sifat fisika dan mekanika dilakukan berdasarkan Japanese Industrial Standard untuk papan partikel (JIS A 5908. Penambahan asam sitrat memperlihatkan kenaikan sifat fisika (penyerapan air dan mekanika papan partikel. Perbedaan ukuran partikel mempengaruhi sifat mekanika papan partikel dimana ukuran partikel kasar memberikan nilai mekanika yang lebih baik dibandingkan dengan ukuran partikel halus. Kualitas papan partikel optimum diperoleh pada kondisi pengempaan 180ºC, penambahan asam sitrat 10% dari partikel ukuran kasar dengan nilai pengembangan tebal 2,4%, penyerapan air 41%, kekuatan rekat internal 0,2 MPa, modulus patah 5,5 MPa, dan modulus elastisitas 1,6 GPa. Kata kunci: asam sitrat, pelepah nipah, konsentrasi asam sitrat, suhu pengempa   Effect of Citric Acid Concentration and Pressing Temperature on the Quality of Particleboard from Nypa Frond Abstract Binderlessboard is one of the potential eco friendly products that can be developed in Indonesia. However, its boards usually have low in dimensional stability. Addition of the chemical agent, such as citric acid, that can improve the dimensional stability is

  17. Nipah virus infects specific subsets of porcine peripheral blood mononuclear cells.

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    Beata Stachowiak

    Full Text Available Nipah virus (NiV, a zoonotic paramyxovirus, is highly contagious in swine, and can cause fatal infections in humans following transmission from the swine host. The main viral targets in both species are the respiratory and central nervous systems, with viremia implicated as a mode of dissemination of NiV throughout the host. The presented work focused on the role of peripheral blood mononuclear cells (PBMC in the viremic spread of the virus in the swine host. B lymphocytes, CD4-CD8-, as well as CD4+CD8- T lymphocytes were not permissive to NiV, and expansion of the CD4+CD8- cells early post infection was consistent with functional humoral response to NiV infection observed in swine. In contrast, significant drop in the CD4+CD8- T cell frequency was observed in piglets which succumbed to the experimental infection, supporting the hypothesis that antibody development is the critical component of the protective immune response. Productive viral replication was detected in monocytes, CD6+CD8+ T lymphocytes and NK cells by recovery of infectious virus in the cell supernatants. Virus replication was supported by detection of the structural N and the non-structural C proteins or by detection of genomic RNA increase in the infected cells. Infection of T cells carrying CD6 marker, a strong ligand for the activated leukocyte cell adhesion molecule ALCAM (CD166 highly expressed on the microvascular endothelial cell of the blood-air and the blood-brain barrier may explain NiV preferential tropism for small blood vessels of the lung and brain.

  18. Nipah Encephalitis – A Dangerous Zooanthroponosis Of Indo-Malaysian Region Of South-Еast Asia

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    E. P. Lukin

    2014-01-01

    Full Text Available The review provides information on a new zooanthroponosis – Nipah encephalitis. Characteristics of the pathogen and its ecology and clinic are presented. The purpose of the review is to get Russian specialist acquainted with new dangerous disease unknown in Russia. The research method – analytical. Infection by humans were first described in Malaysia (1999, after in Bangladesh (2004 and India (2006. The causative agent was identified as a new member of paramyxoviruses and then, together with related Hendra virus, separated in a new genus Henipavirus of the family Paramyxoviridae (2000. Reservoir in nature – fruit bats, predominantly carnivorous flying foxes of 8 species of the genus Pteropus, secondary reservoir – domestic pigs. Nipah virus is highly contagious for humans and swine. The last act as amplifying and reservoir host. The disease inhumans is characterized by symptoms and signs of acute encephalitis and pulmonary insufficiency. Rapidly developing coma, lethality – up to 92,0%. Outbreaks with transmission of the virus from person-to-person are known. Specific treatment has not been developed, prevention is nonspecific.

  19. Antioxidant and Atibacterial Activities of Nipah (Nypa fruticans against Vibrio sp. Isolated From Mud Crab (Scylla sp.

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    Imra Imra

    2016-12-01

    Full Text Available Nipah (Nypa fruticans is the potential plant for source of active compound such as antioksidant and antibacterial substances. The plants are dispersed in Sumatera, Kalimantan, Sulawesi, Maluku and Papua Island. The aim of this research were determine the antioxidant and antibacterial activity from of nipah (fruit and leaf that it extraction with methanol, and than determine toxicity and active compound contained in this extract. Diffusion agar and DPPH method were use for antibacterial and antioxsidant assay, respectively. Antioxsidant activity from nipah’s leaf extract was more effective (22,5 µg/mL than nipah’s fruit extract (415 µg/mL. This activity to be classified to the strong antioxidant activity (IC50<50 µg/mL. The antibacterial activity from leaf extract was strong to inhibited Vibrio sp. with inhibition zone 8,75 mm. The crude extract of nipah’s leaf was toxic with toxicity value is 663,598 µg/mL. Flavonoids, steroids, tanin, saponin and phenol hidroquinon were the active compounds contained in the extract of nipah’s leaf.

  20. Antioxidant and Atibacterial Activities of Nipah (Nypa fruticans against Vibrio sp. Isolated From Mud Crab (Scylla sp.

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    Imra Imra

    2017-02-01

    Full Text Available AbstractNipah (Nypa fruticans is the potential plant for source of active compound such as antioksidant and antibacterial substances. The plants are dispersed in Sumatera, Kalimantan, Sulawesi, Maluku and Papua Island. The aim of this research were determine the antioxidant and antibacterial activity from of nipah (fruit and leaf that it extraction with methanol, and than determine toxicity and active compound contained in this extract. Diffusion agar and DPPH method were use for antibacterial and antioxsidant assay, respectively. Antioxsidant activity from nipah’s leaf extract was more effective (22,5 μg/mL than nipah’s fruit extract (415 μg/mL. This activity to be classified to the strong antioxidant activity (IC50<50 μg/mL. The antibacterial activity from leaf extract was strong to inhibited Vibrio sp. with inhibition zone 8,75 mm. The crude extract of nipah’s leaf was toxic with toxicity value is 663,598 μg/mL. Flavonoids, steroids, tanin, saponin and phenol hidroquinon were the active compounds contained in the extract of nipah’s leaf.

  1. Nipah Virus Transmission from Bats to Humans Associated with Drinking Traditional Liquor Made from Date Palm Sap, Bangladesh, 2011–2014

    Centers for Disease Control (CDC) Podcasts

    2016-06-30

    Sarah Gregory reads an abridged version of the article, Nipah Virus Transmission from Bats to Humans Associated with Drinking Traditional Liquor Made from Date Palm Sap, Bangladesh, 2011–2014.  Created: 6/30/2016 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 6/30/2016.

  2. Clinical Significance of Human Metapneumovirus in Refractory Status Epilepticus and Encephalitis: Case Report and Review of the Literature

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    Aysel Vehapoglu

    2015-01-01

    Full Text Available Encephalitis is a complex neurological disease that is associated with significant morbidity and mortality, and the etiology of the disease is often not identified. Human metapneumovirus (hMPV is a common cause of upper and lower respiratory tract infections in children. Few reports are available showing possible involvement of hMPV in development of neurologic complications. Here, we describe an infant, the youngest case in literature, with refractory status epilepticus and severe encephalitis in whom hMPV was detected in respiratory samples and review diagnostic workup of patient with encephalitis.

  3. Severe metapneumovirus infections among immunocompetent and immunocompromised patients admitted to hospital with respiratory infection.

    Science.gov (United States)

    Souza, Juliana Sinohara; Watanabe, Aripuana; Carraro, Emerson; Granato, Celso; Bellei, Nancy

    2013-03-01

    Human metapneumovirus (hMPV) is considered an important cause of acute respiratory infections. hMPV can cause morbidity in hematopoietic stem cell transplant recipients and recent research has demonstrated that it is an important virus in patients admitted to hospital with respiratory infections and suspected of having pandemic 2009 influenza A (H1N1pdm09) virus. The purpose of this study was to investigate infections caused by hMPV in two groups of patients admitted to hospital: Immunocompromized patients with a potential risk of severe outcomes and immunocompetent patients with severe acute respiratory syndrome. A total of 288 samples were tested: 165 samples were collected from patients with suspected influenza A (H1N1) pdm09 infection during the first pandemic wave in 2009; and 123 samples were collected from patients of a hematopoietic stem cell transplantation program in 2008-2009. Amplification of the hMPV genes was performed by polymerase chain reaction. This was followed by sequencing and phylogenetic analysis. hMPV was detected in 14.2% (41/288) of all samples: 17% (28/165) of immunocompetent patients with suspected H1N1 infection and 10.6% (13/123) among hematopoietic stem cell transplant recipients. hMPV accounted for 12.1% (8/66) of immunocompetent adults patients with severe respiratory infections (median age, 55.9 years). Two hMPV subtypes were identified, A2 (26.9%; 7/26) and B2 (73.1%; 19/26) but no difference was observed between the patient groups in terms of age or immunosuppression level. This study highlights the significance of hMPV in immunocompetent adult patients with severe infections and further investigations are recommended for understanding the impact of this virus.

  4. Modulation of protease activated receptor 1 influences human metapneumovirus disease severity in a mouse model.

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    Laetitia Aerts

    Full Text Available Human metapneumovirus (hMPV infection causes acute respiratory tract infections (RTI which can result in hospitalization of both children and adults. To date, no antiviral or vaccine is available for this common viral infection. Immunomodulators could represent an interesting strategy for the treatment of severe viral infection. Recently, the role of protease-activated receptors (PAR in inflammation, coagulation and infection processes has been of growing interest. Herein, the effects of a PAR1 agonist and a PAR1 antagonist on hMPV infection were investigated in BALB/c mice. Intranasal administration of the PAR1 agonist resulted in increased weight loss and mortality of infected mice. Conversely, the PAR1 antagonist was beneficial to hMPV infection by decreasing weight loss and clinical signs and by significantly reducing pulmonary inflammation, pro-inflammatory cytokine levels (including IL-6, KC and MCP-1 and recruitment of immune cells to the lungs. In addition, a significant reduction in pulmonary viral titers was also observed in the lungs of PAR1 antagonist-treated mice. Despite no apparent direct effect on virus replication during in vitro experiments, an important role for PAR1 in the regulation of furin expression in the lungs was shown for the first time. Further experiments indicated that the hMPV fusion protein can be cleaved by furin thus suggesting that PAR1 could have an effect on viral infectivity in addition to its immunomodulatory properties. Thus, inhibition of PAR1 by selected antagonists could represent an interesting strategy for decreasing the severity of paramyxovirus infections.

  5. Burden of human metapneumovirus infections in patients with cancer: Risk factors and outcomes.

    Science.gov (United States)

    El Chaer, Firas; Shah, Dimpy P; Kmeid, Joumana; Ariza-Heredia, Ella J; Hosing, Chitra M; Mulanovich, Victor E; Chemaly, Roy F

    2017-06-15

    Human metapneumovirus (hMPV) causes upper and lower respiratory tract infections (URIs and LRIs, respectively) in healthy and immunocompromised patients; however, its clinical burden in patients with cancer remains unknown. In a retrospective study of all laboratory-confirmed hMPV infections treated at the authors' institution between April 2012 and May 2015, clinical characteristics, risk factors for progression to an LRI, treatment, and outcomes in patients with cancer were determined. In total, 181 hMPV infections were identified in 90 patients (50%) with hematologic malignancies (HMs), in 57 (31%) hematopoietic cell transplantation (HCT) recipients, and in 34 patients (19%) with solid tumors. Most patients (92%) had a community-acquired infection and presented with URIs (67%), and 43% developed LRIs (59 presented with LRIs and 19 progressed from a URI to an LRI). On multivariable analysis, an underlying HM (adjusted odds ratio [aOR], 3.11; 95% confidence interval [CI], 1.12-8.64; P = .029), nosocomial infection (aOR, 26.9; 95% CI, 2.79-259.75; P = .004), and hypoxia (oxygen saturation [SpO2], ≤ 92%) at presentation (aOR, 9.61; 95% CI, 1.98-46.57; P = .005) were identified as independent factors associated with LRI. All-cause mortality at 30 days from hMPV diagnosis was low (4%), and patients with LRIs had a 10% mortality rate at day 30 from diagnosis; whereas patients with URIs had a 0% mortality rate. hMPV infections in patients with cancer may cause significant morbidity, especially for those with underlying HM who may develop an LRI. Despite high morbidity and the lack of directed antiviral therapy for hMPV infections, mortality at day 30 from this infection remained low in this studied population. Cancer 2017;123:2329-2337. © 2017 American Cancer Society. © 2017 American Cancer Society.

  6. The human metapneumovirus matrix protein stimulates the inflammatory immune response in vitro.

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    Audrey Bagnaud-Baule

    Full Text Available Each year, during winter months, human Metapneumovirus (hMPV is associated with epidemics of bronchiolitis resulting in the hospitalization of many infants. Bronchiolitis is an acute illness of the lower respiratory tract with a consequent inflammation of the bronchioles. The rapid onset of inflammation suggests the innate immune response may have a role to play in the pathogenesis of this hMPV infection. Since, the matrix protein is one of the most abundant proteins in the Paramyxoviridae family virion, we hypothesized that the inflammatory modulation observed in hMPV infected patients may be partly associated with the matrix protein (M-hMPV response. By western blot analysis, we detected a soluble form of M-hMPV released from hMPV infected cell as well as from M-hMPV transfected HEK 293T cells suggesting that M-hMPV may be directly in contact with antigen presenting cells (APCs during the course of infection. Moreover, flow cytometry and confocal microscopy allowed determining that M-hMPV was taken up by dendritic cells (moDCs and macrophages inducing their activation. Furthermore, these moDCs enter into a maturation process inducing the secretion of a broad range of inflammatory cytokines when exposed to M-hMPV. Additionally, M-hMPV activated DCs were shown to stimulate IL-2 and IFN-γ production by allogeneic T lymphocytes. This M-hMPV-mediated activation and antigen presentation of APCs may in part explain the marked inflammatory immune response observed in pathology induced by hMPV in patients.

  7. Solution and crystallographic structures of the central region of the phosphoprotein from human metapneumovirus.

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    Cedric Leyrat

    Full Text Available Human metapneumovirus (HMPV of the family Paramyxoviridae is a major cause of respiratory illness worldwide. Phosphoproteins (P from Paramyxoviridae are essential co-factors of the viral RNA polymerase that form tetramers and possess long intrinsically disordered regions (IDRs. We located the central region of HMPV P (P(ced which is involved in tetramerization using disorder analysis and modeled its 3D structure ab initio using Rosetta fold-and-dock. We characterized the solution-structure of P(ced using small angle X-ray scattering (SAXS and carried out direct fitting to the scattering data to filter out incorrect models. Molecular dynamics simulations (MDS and ensemble optimization were employed to select correct models and capture the dynamic character of P(ced. Our analysis revealed that oligomerization involves a compact central core located between residues 169-194 (P(core, that is surrounded by flexible regions with α-helical propensity. We crystallized this fragment and solved its structure at 3.1 Å resolution by molecular replacement, using the folded core from our SAXS-validated ab initio model. The RMSD between modeled and experimental tetramers is as low as 0.9 Å, demonstrating the accuracy of the approach. A comparison of the structure of HMPV P to existing mononegavirales P(ced structures suggests that P(ced evolved under weak selective pressure. Finally, we discuss the advantages of using SAXS in combination with ab initio modeling and MDS to solve the structure of small, homo-oligomeric protein complexes.

  8. Methyltransferase-defective avian metapneumovirus vaccines provide complete protection against challenge with the homologous Colorado strain and the heterologous Minnesota strain

    Science.gov (United States)

    Avian metapneumovirus (aMPV), also known as avian pneumovirus or turkey rhinotracheitis virus, is the causative agent of turkey rhinotracheitis, and is associated with swollen head syndrome in chickens. Since its discovery in the 1970s, aMPV has been recognized as an economically important pathogen ...

  9. A community study of clinical traits and risk factors for human metapneumovirus and respiratory syncytial virus infection during the first year of life

    DEFF Research Database (Denmark)

    von Linstow, Marie-Louise; Høgh, Mette; Nordbø, Svein;

    2008-01-01

    Human metapneumovirus (hMPV) and respiratory syncytial virus (RSV) are important respiratory pathogens with similar symptomatology. The aim of this prospective birth cohort study was to identify risk factors for an hMPV or RSV infection during the first year of life in unselected healthy children...

  10. Nipah Desease: A New Type of Zoonosis%一种新型的人畜互传病--立百病

    Institute of Scientific and Technical Information of China (English)

    刘玉如; 佘锐萍; 李艺娟

    2001-01-01

    @@ 立百病(Nipah disease)是1998年在马来西亚首次发现的一种严重危害猪和人类的病毒性人畜共患病.1998年10月至1999年5月期间,立百病在马来西亚的猪群和人群中大规模暴发流行,并殃及到新加坡,导致105人死亡,116万头猪被捕杀.2000年2月以来,该病再度在马来西亚流行,到目前为止已有1人死亡,1728头猪被捕杀,引起该国和周边国家的广泛关注.

  11. Towards understanding of Nipah virus attachment protein assembly and the role of protein affinity and crowding for membrane curvature events.

    Energy Technology Data Exchange (ETDEWEB)

    Stachowiak, Jeanne C.; Hayden, Carl C.; Negrete, Oscar.; Davis, Ryan Wesley; Sasaki, Darryl Y

    2013-10-01

    Pathogenic viruses are a primary threat to our national security and to the health and economy of our world. Effective defense strategies to combat viral infection and spread require the development of understanding of the mechanisms that these pathogens use to invade the host cell. We present in this report results of our research into viral particle recognition and fusion to cell membranes and the role that protein affinity and confinement in lipid domains plays in membrane curvature in cellular fusion and fission events. Herein, we describe 1) the assembly of the G attachment protein of Nipah virus using point mutation studies to define its role in viral particle fusion to the cell membrane, 2) how lateral pressure of membrane bound proteins induce curvature in model membrane systems, and 3) the role of membrane curvature in the selective partitioning of molecular receptors and specific affinity of associated proteins.

  12. 尼帕病的流行与防控技术研究现状%Epidemic Status and Prevention Technology of Nipah Disease

    Institute of Scientific and Technical Information of China (English)

    赵永刚; 樊晓旭; 刘学东; 赵明; 吴晓东; 王志亮

    2016-01-01

    The first outbreak of Nipah disease was found in Malaysia. It was not only a serious harm to the pig industry, but also a serious impact on human health. There were numerous reports of Nipah disease outbreaks in Bangladesh and India every year. Phylogenetic analysis of viral N gene sequences revealed that the evolution of the virus was divided into two developmental directions. The effective anti Nipah virus drugs and vaccines havet not been developed yet. Chi-na has initially established detective methods of Nipah virus such as real-time quantitative RT-PCR and indirect ELISA. Although China is free of Nipah,the risk of introduction is not negligible. It is suggested that Nipah should be brought into the scope of comprehensive prevention and control management.%尼帕病首次暴发于东南亚地区的马来西亚,不但严重危害养猪业,而且严重影响着人类的健康。目前,孟加拉国、印度每年都有尼帕病暴发的报道。通过病毒N基因序列的系统进化分析发现,尼帕病毒进化分为两个发展方向。目前还没有研制出有效的抗尼帕病毒药物、疫苗。我国已经初步建立了实时定量RT-PCR和间接ELISA检测尼帕病毒的方法,虽然还没有发现该病的报道,但存在传入的风险。提议我国应将尼帕病纳入综合防控管理范围,积极开展尼帕病防控的宣传与培训,加强尼帕病的技术研究和监测,并制订相关的应急预案。

  13. The mechanism by which the Nipah virus invades host cells%尼帕病毒侵入宿主细胞机制的研究进展

    Institute of Scientific and Technical Information of China (English)

    王磊; 王铁成; 杨松涛; 夏咸柱; 李天松; 王照; 刘玉秀; 冯娜; 王胜乐; 许薇薇; 姜雪; 高玉伟

    2012-01-01

    尼帕病毒属于副粘病毒科病毒,可以引起人和猪等多种动物中枢神经系统、呼吸系统病变的急性、高度致死性传染性病毒性疾病,首次报道于1999年的马来群岛,再次暴发于东南亚,病死率高达40%,并且怀疑可以在人与人之间进行传播.本文综述了尼帕病毒侵入宿主细胞及其F蛋白介导的细胞融合机制的研究进展.%Nipah virus belongs to the Paramyxoviridae family of viruses. Highly fatal and highly contagious, Nipah virus infection can cause acute symptoms of the central nervous system and respiratory system in animals such as pigs and humans. First reported in Malaysia in 1999, the viral infection broke out again in Southeast Asia with a mortality rate of over 40%. There are doubts as to whether the infection can be spread from person—to—person. This paper reviews research on the virus' mechanism of invading host ceils and cell fusion mediated by the F protein of the Nipah virus.

  14. Immunogenicity and efficacy of alphavirus-derived replicon vaccines for respiratory syncytial virus and human metapneumovirus in nonhuman primates.

    Science.gov (United States)

    Bates, John T; Pickens, Jennifer A; Schuster, Jennifer E; Johnson, Monika; Tollefson, Sharon J; Williams, John V; Davis, Nancy L; Johnston, Robert E; Schultz-Darken, Nancy; Slaughter, James C; Smith-House, Frances; Crowe, James E

    2016-02-10

    Human respiratory syncytial virus (hRSV) and human metapneumovirus (hMPV) are major causes of illness among children, the elderly, and the immunocompromised. No vaccine has been licensed for protection against either of these viruses. We tested the ability of two Venezuelan equine encephalitis virus-based viral replicon particle (VEE-VRP) vaccines that express the hRSV or hMPV fusion (F) protein to confer protection against hRSV or hMPV in African green monkeys. Animals immunized with VEE-VRP vaccines developed RSV or MPV F-specific antibodies and serum neutralizing activity. Compared to control animals, immunized animals were better able to control viral load in the respiratory mucosa following challenge and had lower levels of viral genome in nasopharyngeal and bronchoalveolar lavage fluids. The high level of immunogenicity and protective efficacy induced by these vaccine candidates in nonhuman primates suggest that they hold promise for further development.

  15. First Report of Avian Metapneumovirus Subtype B Field Strain in a Romanian Broiler Flock During an Outbreak of Respiratory Disease.

    Science.gov (United States)

    Franzo, Giovanni; Tucciarone, Claudia Maria; Enache, Mirel; Bejan, Violeta; Ramon, Gema; Koutoulis, Konstantinos C; Cecchinato, Mattia

    2017-06-01

    Avian metapneumovirus (aMPV) represents one of the most prevalent diseases of turkey, especially in combination with other pathogens, and its frequency is also increasing among chickens. Despite this evidence, epidemiologic data are poor and scattered, severely preventing control of the disease even in highly developed areas such as Europe. In the present study, the detection and characterization of an aMPV subtype B strain circulating in a vaccinated but symptomatic Romanian broiler flock is reported for the first time. The phylogenetic analysis based on the partial G gene sequence demonstrates the close relationship of the Romanian virus with a group of recently emerged Italian field strains for which vaccine-induced protection was experimentally proven to be partial. These preliminary results allow us to hypothesize the spreading of vaccine-escaping aMPV subtype B strains through Europe and, consequently, dictate the carrying out of a more systematic survey to confirm this theory and enforce adequate countermeasures.

  16. Limited inter- and intra-patient sequence diversity of the genetic lineage a human metapneumovirus fusion gene

    DEFF Research Database (Denmark)

    Winther, T.N.; Madsen, C.D.; Pedersen, Anders Gorm;

    2005-01-01

    Human metapneumovirus (hMPV) is associated with respiratory tract illness especially in young children. Two hMPV genetic lineages, A and B, and four sublineages A1, A2 and B1, B2 have been defined. Infection with hMPV occurs through membrane fusion mediated by the hMPV fusion (F) protein. In this...... diversity observed lay emphasis on the hMPV F gene as a putative target for future vaccine development....... been infected with at least two viruses. Several independent viruses contained premature stop codons in exactly identical positions resulting in truncated fusion proteins. Possibly this is a mechanism for immune system evasion. The F protein is a major antigenic determinant, and the limited sequence...

  17. Antiviral Activity of Favipiravir (T-705) against a Broad Range of Paramyxoviruses In Vitro and against Human Metapneumovirus in Hamsters.

    Science.gov (United States)

    Jochmans, D; van Nieuwkoop, S; Smits, S L; Neyts, J; Fouchier, R A M; van den Hoogen, B G

    2016-08-01

    The clinical impact of infections with respiratory viruses belonging to the family Paramyxoviridae argues for the development of antiviral therapies with broad-spectrum activity. Favipiravir (T-705) has demonstrated potent antiviral activity against multiple RNA virus families and is presently in clinical evaluation for the treatment of influenza. Here we demonstrate in vitro activity of T-705 against the paramyxoviruses human metapneumovirus (HMPV), respiratory syncytial virus, human parainfluenza virus, measles virus, Newcastle disease virus, and avian metapneumovirus. In addition, we demonstrate activity against HMPV in hamsters. T-705 treatment inhibited replication of all paramyxoviruses tested in vitro, with 90% effective concentration (EC90) values of 8 to 40 μM. Treatment of HMPV-challenged hamsters with T-705 at 200 mg/kg of body weight/day resulted in 100% protection from infection of the lungs. In all treated and challenged animals, viral RNA remained detectable in the respiratory tract. The observation that T-705 treatment had a significant effect on infectious viral titers, with a limited effect on viral genome titers, is in agreement with its proposed mode of action of viral mutagenesis. However, next-generation sequencing of viral genomes isolated from treated and challenged hamsters did not reveal (hyper)mutation. Polymerase activity assays revealed a specific effect of T-705 on the activity of the HMPV polymerase. With the reported antiviral activity of T-705 against a broad range of RNA virus families, this small molecule is a promising broad-range antiviral drug candidate for limiting the viral burden of paramyxoviruses and for evaluation for treatment of infections with (re)emerging viruses, such as the henipaviruses.

  18. In-Vitro Antiviral Activities of Extracts of Plants of The Brazilian Cerrado against the Avian Metapneumovirus (aMPV

    Directory of Open Access Journals (Sweden)

    LK Kohn

    2015-09-01

    Full Text Available ABSTRACTAvian metapneumovirus (aMPV is a negative-sense single-stranded RNA enveloped virus of the Metapneumovirus genus belonging to theParamyxoviridae family. This virus may cause significant economic losses to the poultry industry, despite vaccination, which is the main tool for controlling and preventing aMPV. The aim of this study was to evaluate the antiviral activity of extracts of four different native plants of the Brazilian Cerrado against aMPV. The antiviral activity against aMPV was determined by titration. This technique measures the ability of plant extract dilutions (25 to 2.5 µg mL-1 to inhibit the cytopathic effect (CPE of the virus, expressed as inhibition percentage (IP. The maximum nontoxic concentration (MNTC of the extracts used in antiviral assay was 25 µg mL-1for Aspidosperma tomentosumand Gaylussacia brasiliensis, and 2.5 µg mL-1for Arrabidaea chicaand Virola sebifera. Twelve different extracts derived from four plant species collected from the Brazilian Cerrado were screened for antiviral activity against aMPV. G. brasiliensis, A. chica,and V. sebifera extracts presented inhibition rates of 99% in the early viral replication stages, suggesting that these extracts act during the adsorption phase. On the other hand, A. tomentosum inhibited 99% virus replication after the virus entered the cell. The biomonitored fractioning of extracts active against aMPV may be a tool to identify the active compounds of plant extracts and to determine their precise mode of action.

  19. [Phylogenetic variability of human metapneumovirus strains circulating in Turkey during two consecutive epidemic seasons].

    Science.gov (United States)

    Bayrakdar, Fatma; Altaş, Ayşe Başak; Korukluoğlu, Gülay

    2016-01-01

    Human metapneumovirus (HMPV), classified in Paramyxoviridae family, phylogenetically consists of two major groups namely A and B, with genetic lineages of A1, A2 (comprises of sublineages A2a and A2b) and B1, B2. Although detailed evaluation on phylogenetic analysis of HMPV has been described in other countries, there are no data from Turkey on this subject. The aim of this study was to demonstrate for the first time, the phylogenetic diversity of HMPV strains circulating in Turkey during two consecutive epidemic seasons. A total of 2900 upper respiratory tract samples collected from patients with respiratory illness were evaluated between January 2011 and December 2013, without any special selection criteria. The presence of respiratory viruses in the samples were detected by real-time multiplex polymerase chain reaction (FTD® Respiratory Pathogens 21 Multiplex RT-PCR, Fast Track Diagnostics, Luxemburg), and 76 (2.6%) samples positive for HMPV were included in the study. HPMV nucleocapsid (N) (nt: 454-878) and fusion (F) (nt: 3624-4130) genes were selected for phylogenetic analysis. In sequence analysis, F and N gene sequences could only be obtained successfully from 46 out of 76 HMPV positive samples. According to sequences obtained, 54.3% belonged to B2, 17.4% to B1, 4.3% to A1, 4.3% to A2a, and 20% to A2b. In 2011, the A2b sublineage was predominant, while in 2012 and 2013, B2 lineages were predominant together with the B1 lineage. The A1 lineage was observed only in 2013. For the F gene fragment, nucleotide distance between group A and B was in the range of 0.138-0.168, however aminoacid distance amongst Turkish HMPV sequences were in the range of 0.028-0.042. For the N gene fragment, nucleotide distance between group A and B was in the range of 0.141-0.163, but aminoacid distance between group A and B was in the range of 0.037-0.050. Nucleotide diversity was higher than aminoacid diversity between and within lineages found in this study. This result

  20. A neutralization test for specific detection of Nipah virus antibodies using pseudotyped vesicular stomatitis virus expressing green fluorescent protein.

    Science.gov (United States)

    Kaku, Yoshihiro; Noguchi, Akira; Marsh, Glenn A; McEachern, Jennifer A; Okutani, Akiko; Hotta, Kozue; Bazartseren, Boldbaatar; Fukushi, Shuetsu; Broder, Christopher C; Yamada, Akio; Inoue, Satoshi; Wang, Lin-Fa

    2009-09-01

    Nipah virus (NiV) is a new zoonotic paramyxovirus that emerged in 1998 and is now classified in the genus Henipavirus along with the closely related Hendra virus (HeV). NiV is highly pathogenic in several vertebrate species including humans, and the lack of available vaccines or specific treatment restricts it to biosafety level 4 (BSL4) containment. A serum neutralization test was developed for measuring NiV neutralizing antibodies under BSL2 conditions using a recombinant vesicular stomatitis virus (VSV) expressing green fluorescent protein (GFP) and bearing the F and G proteins of NiV (VSV-NiV-GFP). The neutralization titers were obtained by counting GFP-expressing cells or by measuring fluorescence. The performance of this new assay was compared against the conventional test using live NiV with panels of sera from several mammalian species, including sera from NiV outbreaks, experimental infections, as well as HeV-specific sera. The results obtained with the VSV-NiV-GFP based test correlated with those obtained using live NiV. Using a 50% reduction in VSV-NiV-GFP infected cells as the cut-off for neutralization, this new assay demonstrated its potential as an effective tool for detecting NiV neutralizing antibodies under BSL2 containment with greater speed, sensitivity and safety as compared to the conventional NiV serum neutralization test.

  1. Piloting the promotion of bamboo skirt barriers to prevent Nipah virus transmission through date palm sap in Bangladesh.

    Science.gov (United States)

    Nahar, Nazmun; Mondal, Utpal Kumar; Hossain, M Jahangir; Khan, M Salah Uddin; Sultana, Rebeca; Gurley, Emily S; Luby, Stephen P

    2014-12-01

    Drinking raw date palm sap contaminated with infected fruit bat saliva or urine is an important mode of Nipah virus transmission to humans in Bangladesh. Bamboo skirts are an effective way to interrupt bat access to the sap. We conducted a study from November 2008 to March 2009 to explore the effectiveness of higher- and lower-intensity interventions by promoting bamboo skirt preparation and use among sap harvesters (gachhis). We spent 280 person-hours in two villages for the higher-intensity intervention and half that amount of time in two other villages for the lower-intensity intervention. To evaluate the interventions we followed up all gachhis once a month for three months. A high percentage of gachhis (83% in higher-, 65% in lower-intensity interventions) prepared and used a skirt of bamboo or other materials - jute stalk, dhoincha (Sesbania aculeata), or polythene - at least once after intervention. In general, 15% of gachhis consistently used skirts throughout the sap collection season. The intensive nature of this intervention is very expensive for a large-scale programme. Future efforts should focus on developing a low-cost behaviour change intervention and evaluate if it reduces the human exposure to potentially contaminated fresh date palm sap.

  2. Agricultural intensification, priming for persistence and the emergence of Nipah virus: a lethal bat-borne zoonosis.

    Science.gov (United States)

    Pulliam, Juliet R C; Epstein, Jonathan H; Dushoff, Jonathan; Rahman, Sohayati A; Bunning, Michel; Jamaluddin, Aziz A; Hyatt, Alex D; Field, Hume E; Dobson, Andrew P; Daszak, Peter

    2012-01-07

    Emerging zoonoses threaten global health, yet the processes by which they emerge are complex and poorly understood. Nipah virus (NiV) is an important threat owing to its broad host and geographical range, high case fatality, potential for human-to-human transmission and lack of effective prevention or therapies. Here, we investigate the origin of the first identified outbreak of NiV encephalitis in Malaysia and Singapore. We analyse data on livestock production from the index site (a commercial pig farm in Malaysia) prior to and during the outbreak, on Malaysian agricultural production, and from surveys of NiV's wildlife reservoir (flying foxes). Our analyses suggest that repeated introduction of NiV from wildlife changed infection dynamics in pigs. Initial viral introduction produced an explosive epizootic that drove itself to extinction but primed the population for enzootic persistence upon reintroduction of the virus. The resultant within-farm persistence permitted regional spread and increased the number of human infections. This study refutes an earlier hypothesis that anomalous El Niño Southern Oscillation-related climatic conditions drove emergence and suggests that priming for persistence drove the emergence of a novel zoonotic pathogen. Thus, we provide empirical evidence for a causative mechanism previously proposed as a precursor to widespread infection with H5N1 avian influenza and other emerging pathogens.

  3. Unraveling a three-step spatiotemporal mechanism of triggering of receptor-induced Nipah virus fusion and cell entry.

    Directory of Open Access Journals (Sweden)

    Qian Liu

    Full Text Available Membrane fusion is essential for entry of the biomedically-important paramyxoviruses into their host cells (viral-cell fusion, and for syncytia formation (cell-cell fusion, often induced by paramyxoviral infections [e.g. those of the deadly Nipah virus (NiV]. For most paramyxoviruses, membrane fusion requires two viral glycoproteins. Upon receptor binding, the attachment glycoprotein (HN/H/G triggers the fusion glycoprotein (F to undergo conformational changes that merge viral and/or cell membranes. However, a significant knowledge gap remains on how HN/H/G couples cell receptor binding to F-triggering. Via interdisciplinary approaches we report the first comprehensive mechanism of NiV membrane fusion triggering, involving three spatiotemporally sequential cell receptor-induced conformational steps in NiV-G: two in the head and one in the stalk. Interestingly, a headless NiV-G mutant was able to trigger NiV-F, and the two head conformational steps were required for the exposure of the stalk domain. Moreover, the headless NiV-G prematurely triggered NiV-F on virions, indicating that the NiV-G head prevents premature triggering of NiV-F on virions by concealing a F-triggering stalk domain until the correct time and place: receptor-binding. Based on these and recent paramyxovirus findings, we present a comprehensive and fundamentally conserved mechanistic model of paramyxovirus membrane fusion triggering and cell entry.

  4. Impact of human metapneumovirus infection on in and outpatients for the years 2006-2008 in Southern Brazil

    Directory of Open Access Journals (Sweden)

    Maria C Debur

    2010-12-01

    Full Text Available The human metapneumovirus (hMPV, member of the Paramyxoviridae family, has been reported as an important agent involved with acute respiratory infections (ARIs. The aim of this study is to identify hMPV as the etiological agent of ARIs on in and outpatients in the city of Curitiba, Southern Brazil, and describe clinical data of hMPV subtyping. A retrospective study was performed in 1,572 respiratory samples over a period of three years. hMPV was detected by reverse transcription-polymerase chain reaction and subtyping was performed by nucleotide sequencing. hMPV was present in 61 (3.9% samples and subtypes A1, A2a, B1 and B2 were detected. The incidence of hMPV was higher in outpatients (5.9%, whose mean age was 19.7 years (range 6 months-75 years old, than in inpatients (3%, whose mean age was 7.6 months (range 1 month-26 years old. The outpatients had upper respiratory tract infections with flu-like symptoms and all hospitalized children had lower respiratory tract infections. A pediatric patient died from complications associated with hMPV A2a infection. hMPV has been reported as a respiratory pathogen in all age groups. No correlation was observed between viral subtype and disease severity in the samples of this study.

  5. Small hydrophobic protein of human metapneumovirus does not affect virus replication and host gene expression in vitro.

    Directory of Open Access Journals (Sweden)

    Miranda de Graaf

    Full Text Available Human metapneumovirus (HMPV encodes a small hydrophobic (SH protein of unknown function. HMPV from which the SH open reading frame was deleted (HMPVΔSH was viable and displayed similar replication kinetics, cytopathic effect and plaque size compared with wild type HMPV in several cell-lines. In addition, no differences were observed in infection efficiency or cell-to-cell spreading in human primary bronchial epithelial cells (HPBEC cultured at an air-liquid interphase. Host gene expression was analyzed in A549 cells infected with HMPV or HMPVΔSH using microarrays and mass spectrometry (MS based techniques at multiple time points post infection. Only minor differences were observed in mRNA or protein expression levels. A possible function of HMPV SH as apoptosis blocker, as proposed for several members of the family Paramyxoviridae, was rejected based on this analysis. So far, a clear phenotype of HMPV SH deletion mutants in vitro at the virus and host levels is absent.

  6. Quantitative analysis of Nipah virus proteins released as virus-like particles reveals central role for the matrix protein

    Directory of Open Access Journals (Sweden)

    Eaton Bryan T

    2007-01-01

    Full Text Available Abstract Background Nipah virus (NiV is an emerging paramyxovirus distinguished by its ability to cause fatal disease in both animal and human hosts. Together with Hendra virus (HeV, they comprise the genus Henipavirus in the Paramyxoviridae family. NiV and HeV are also restricted to Biosafety Level-4 containment and this has hampered progress towards examining details of their replication and morphogenesis. Here, we have established recombinant expression systems to study NiV particle assembly and budding through the formation of virus-like particles (VLPs. Results When expressed by recombinant Modified Vaccinia virus Ankara (rMVA or plasmid transfection, individual NiV matrix (M, fusion (F and attachment (G proteins were all released into culture supernatants in a membrane-associated state as determined by sucrose density gradient flotation and immunoprecipitation. However, co-expression of F and G along with M revealed a shift in their distribution across the gradient, indicating association with M in VLPs. Protein release was also altered depending on the context of viral proteins being expressed, with F, G and nucleocapsid (N protein reducing M release, and N release dependent on the co-expression of M. Immunoelectron microscopy and density analysis revealed VLPs that were similar to authentic virus. Differences in the budding dynamics of NiV proteins were also noted between rMVA and plasmid based strategies, suggesting that over-expression by poxvirus may not be appropriate for studying the details of recombinant virus particle assembly and release. Conclusion Taken together, the results indicate that NiV M, F, and G each possess some ability to bud from expressing cells, and that co-expression of these viral proteins results in a more organized budding process with M playing a central role. These findings will aid our understanding of paramyxovirus particle assembly in general and could help facilitate the development of a novel vaccine

  7. Raw Sap Consumption Habits and Its Association with Knowledge of Nipah Virus in Two Endemic Districts in Bangladesh.

    Science.gov (United States)

    Nahar, Nazmun; Paul, Repon C; Sultana, Rebeca; Gurley, Emily S; Garcia, Fernando; Abedin, Jaynal; Sumon, Shariful Amin; Banik, Kajal Chandra; Asaduzzaman, Mohammad; Rimi, Nadia Ali; Rahman, Mahmudur; Luby, Stephen P

    2015-01-01

    Human Nipah virus (NiV) infection in Bangladesh is a fatal disease that can be transmitted from bats to humans who drink contaminated raw date palm sap collected overnight during the cold season. Our study aimed to understand date palm sap consumption habits of rural residents and factors associated with consumption. In November-December 2012 the field team interviewed adult respondents from randomly selected villages from Rajbari and Kushtia Districts in Bangladesh. We calculated the proportion of people who consumed raw sap and had heard about a disease from raw sap consumption. We assessed the factors associated with raw sap consumption by calculating prevalence ratios (PR) adjusted for village level clustering effects. Among the 1,777 respondents interviewed, half (50%) reported drinking raw sap during the previous sap collection season and 37% consumed raw sap at least once per month. Few respondents (5%) heard about NiV. Thirty-seven percent of respondents reported hearing about a disease transmitted through raw sap consumption, inclusive of a 10% who related it with milder illness like diarrhea, vomiting or indigestion rather than NiV. Respondents who harvested date palm trees in their household were more likely to drink sap than those who did not own date palm trees (79% vs. 65% PR 1.2, 95% CI 1.1-1.3, psap was available, respondents who heard about a disease from raw sap consumption were just as likely to drink it as those who did not hear about a disease (69% vs. 67%, PR 1.0, 95% CI 0.9-1.1, p = 0.512). Respondents' knowledge of NiV was low. They might not have properly understood the risk of NiV, and were likely to drink sap when it was available. Implementing strategies to increase awareness about the risks of NiV and protect sap from bats might reduce the risk of NiV transmission.

  8. A Randomized Controlled Trial of Interventions to Impede Date Palm Sap Contamination by Bats to Prevent Nipah Virus Transmission in Bangladesh

    Science.gov (United States)

    Khan, Salah Uddin; Gurley, Emily S.; Hossain, M. Jahangir; Nahar, Nazmun; Sharker, M. A. Yushuf; Luby, Stephen P.

    2012-01-01

    Background Drinking raw date palm sap is a risk factor for human Nipah virus (NiV) infection. Fruit bats, the natural reservoir of NiV, commonly contaminate raw sap with saliva by licking date palm’s sap producing surface. We evaluated four types of physical barriers that may prevent bats from contacting sap. Methods During 2009, we used a crossover design and randomly selected 20 date palm sap producing trees and observed each tree for 2 nights: one night with a bamboo skirt intervention applied and one night without the intervention. During 2010, we selected 120 trees and randomly assigned four types of interventions to 15 trees each: bamboo, dhoincha (local plant), jute stick and polythene skirts covering the shaved part, sap stream, tap and collection pot. We enrolled the remaining 60 trees as controls. We used motion sensor activated infrared cameras to examine bat contact with sap. Results During 2009 bats contacted date palm sap in 85% of observation nights when no intervention was used compared with 35% of nights when the intervention was used [psap when the skirt did not entirely cover the sap producing surface. Therefore, in 2010 we requested the sap harvesters to use larger skirts. During 2010 bats contacted date palm sap [2% vs. 83%, psap in trees with bamboo (psap during one night (7%) with the jute stick skirt (psap producing areas of a tree effectively prevented bat-sap contact. Community interventions should promote applying these skirts to prevent occasional Nipah spillovers to human. PMID:22905160

  9. A community study of clinical traits and risk factors for human metapneumovirus and respiratory syncytial virus infection during the first year of life

    DEFF Research Database (Denmark)

    Linstow, Michael Ernst Von; Hogh, M.; Nordbo, S.A.;

    2008-01-01

    Human metapneumovirus (hMPV) and respiratory syncytial virus (RSV) are important respiratory pathogens with similar symptomatology. The aim of this prospective birth cohort study was to identify risk factors for an hMPV or RSV infection during the first year of life in unselected healthy children......% CI:0.09-0.85). Risk factors for RSV hospitalisation (n = 11) were: (1) older siblings (OR = 4.49; 95% CI: 1.08-18.73) and (2) smoking in the household (OR = 5.06; 95% CI: 1.36-18.76). Exclusive breastfeeding for the first 14 days of life protected against hospitalisation (OR = 0.21; 95% CI:0...

  10. [Prokaryotic expression for fusion protein of human metapneumovirus and its preliminary application as an antigen for antibody detection].

    Science.gov (United States)

    Zhu, Ru-nan; Qian, Yuan; Zhao, Lin-qing; Sun, Yu; Deng, Jie; Wang, Fang

    2011-03-01

    To understand the effectiveness of prokaryotic expression of fusion protein (F) of human metapneumovirus (hMPV) and its application as antigen, F proteins from different genotypes of hMPV were expressed in prokaryotic expression system and purified by Ni-NTA affinity chromatography column. According to the hydrophobicity, antigen index and surface probability of F protein, the subunit 1 (F1) region of F protein was generated and expressed in E. Coil. BL21(DE3). The 6-His-F1 proteins with molecular weight of approximately 37 kD generated from hMPV of two genotypes were expressed efficiently mainly in inclusion body. The antigenicity and specificity of the expressed proteins were tested and confirmed by Western Blot using polyclonal antibody against hMPV and one serum specimen from a patient with confirmed hMPV acute infection,and polyclonal antibodies against human respiratory syncytial virus and parainfluenza virus 2 and 3. The results of preliminary use of the expressed proteins for detecting antibodies against hMPV in 457 serum specimens collected from different age groups in Beijing indicated that 66%-67% of sera in all age groups were positive. The positive rate of antibodies declined in children in age groups from birth to 2-year-old and then rose along with the increase in age, in which the lowest was in age group from 1 to 2-year-old and the highest in newborn and people older than 60 years. The data indicated the existence of maternal transferred antibodies against hMPV in infants and the risk of hMPV infections in children younger than 2 years old.

  11. Comparing Human Metapneumovirus and Respiratory Syncytial Virus: Viral Co-Detections, Genotypes and Risk Factors for Severe Disease

    Science.gov (United States)

    Moe, Nina; Krokstad, Sidsel; Stenseng, Inger Heimdal; Christensen, Andreas; Skanke, Lars Høsøien; Risnes, Kari Ravndal; Nordbø, Svein Arne; Døllner, Henrik

    2017-01-01

    Background It is unclarified as to whether viral co-detection and human metapneumovirus (HMPV) genotypes relate to clinical manifestations in children with HMPV and lower respiratory tract infection (LRTI), and if the clinical course and risk factors for severe LRTI differ between HMPV and respiratory syncytial virus (RSV). Methods We prospectively enrolled hospitalized children aged <16 years with LRTI from 2006 to 2015. Children were clinically examined, and nasopharyngeal aspirates were analyzed using semi-quantitative, real-time polymerase chain reaction tests for HMPV, RSV and 17 other pathogens. HMPV-positive samples were genotyped. Results A total of 171 children had HMPV infection. HMPV-infected children with single virus (n = 106) and co-detections (n = 65) had similar clinical manifestations. No clinical differences were found between HMPV genotypes A (n = 67) and B (n = 80). The HMPV-infected children were older (median 17.2 months) than RSV-infected children (median 7.3 months, n = 859). Among single virus-infected children, no differences in age-adjusted LRTI diagnoses were found between HMPV and RSV. Age was an important factor for disease severity among single virus-infected children, where children <6 months old with HMPV had a milder disease than those with RSV, while in children 12–23 months old, the pattern was the opposite. In multivariable logistic regression analysis for each virus type, age ≥12 months (HMPV), and age <6 months (RSV), prematurity, ≥1 chronic disease and high viral loads of RSV, but not high HMPV viral loads, were risk factors for severe disease. Conclusions Among hospitalized children with LRTI, HMPV manifests independently of viral co-detections and HMPV genotypes. Disease severity in HMPV- and RSV-infected children varies in relation to age. A history of prematurity and chronic disease increases the risk of severe LRTI among HMPV- and RSV-infected children. PMID:28095451

  12. Detection of human bocavirus and human metapneumovirus by real-time PCR from patients with respiratory symptoms in Southern Brazil

    Directory of Open Access Journals (Sweden)

    Diogo André Pilger

    2011-02-01

    Full Text Available The introduction of newer molecular methods has led to the discovery of new respiratory viruses, such as human metapneumovirus (hMPV and human bocavirus (hBoV, in respiratory tract specimens. We have studied the occurrence of hMPV and hBoV in the Porto Alegre (PA metropolitan area, one of the southernmost cities of Brazil, evaluating children with suspected lower respiratory tract infection from May 2007-June 2008. A real-time polymerase chain reaction method was used for amplification and detection of hMPV and hBoV and to evaluate coinfections with respiratory syncytial virus (RSV, influenza A and B, parainfluenza 1, 2 and 3, human rhinovirus and human adenovirus. Of the 455 nasopharyngeal aspirates tested, hMPV was detected in 14.5% of samples and hBoV in 13.2%. A unique causative viral agent was identified in 46.2% samples and the coinfection rate was 43.7%. For hBoV, 98.3% of all positive samples were from patients with mixed infections. Similarly, 84.8% of all hMPV-positive results were also observed in mixed infections. Both hBoV and hMPV usually appeared with RSV. In summary, this is the first confirmation that hMPV and hBoV circulate in PA; this provides evidence of frequent involvement of both viruses in children with clinical signs of acute viral respiratory tract infection, although they mainly appeared as coinfection agents.

  13. Characterization of human metapneumovirus from pediatric patients with acute respiratory infections in a 4-year period in Beijing, China

    Institute of Scientific and Technical Information of China (English)

    ZHU Ru-nan; QIAN Yuan; ZHAO Lin-qing; DENG Jie; SUN Yu; WANG Fang; LIAO Bin; LI Yan; HUANG Rong-yan

    2011-01-01

    Background Human metapneumovirus (hMPV) was discovered by scientists in the Netherlands as a novel respiratory virus in 2001 and had been found in children with acute respiratory tract infections (ARTI) in China. The objective of this study was to determine the importance of hMPV infection in children in Beijing and the genotypes of the circulating virus by the surveillance during a four-consecutive-year period.Methods Clinical specimens collected from children with ARTI from January 2006 to December 2009 were tested for hMPV by RT-PCR using primers targeting the matrix (M) gene, followed by genotyping of hMPV directly from positive samples by diplex PCR with primers for glycoprotein (G) genes. Sequence analysis was used for genotyping of those un-typable samples. Common respiratory viruses in these clinical specimens were tested by virus isolation and antigen detection, in addition to hMPV detection.Results Of 4730 tested specimens, 191 (4.0%) were positive for hMPV and 62.8% of 191 were identified as genotype A. The positive rate of hMPV from hospitalized patients was higher than that from outpatients each year. Most of hMPV positive children were under five years old. The peak of hMPV activity mostly occurred in late spring and overlapped with or followed that of respiratory syncytial virus (RSV) and followed by parainfluenza virus 3. Of hMPV infected cases,68.6% were lower respiratory tract infection, among which 79.4% were hospitalized, and upper respiratory tract infection was diagnosed for 31.4% of hMPV infected children. The 9.4% of hMPV positive samples were found to co-exist with other respiratory viruses.Conclusions hMPV was an important pathogen for ARTI in pediatric patients, especially those under five years old.Both genotypes A and B circulated simultaneously in Beijing.

  14. Visual detection of the human metapneumovirus using reverse transcription loop-mediated isothermal amplification with hydroxynaphthol blue dye

    Directory of Open Access Journals (Sweden)

    Wang Xiang

    2012-07-01

    Full Text Available Abstract Background Human metapneumovirus (hMPV is a major cause of acute respiratory infections ranging from wheezing to bronchiolitis and pneumonia in children worldwide. The objective of this study is to develop a visual reverse transcription loop-mediated isothermal amplification (RT-LAMP assay for the detection of hMPV and applied to the clinical samples. Results In this study, visual RT-LAMP assay for hMPV was performed in one step with the addition of hydroxynaphthol blue (HNB, and were used to detect respiratory samples. Six primers, including two outer primers (F3 and B3, two inner primers (FIP, BIP and two loop primers (LF and LB, were designed for hMPV N gene by the online software. Moreover, the RT-LAMP assay showed good specificity and no cross-reactivity was observed with human rhinovirus (HRV, human respiratory syncytial Virus (RSV, or influenza virus A/PR/8/34 (H1N1. The detection limit of the RT-LAMP assay was approximately ten viral RNA copies, lower than that of traditional reverse transcriptase polymerase chain reaction (RT-PCR 100 RNA copies. In the 176 nasopharyngeal samples, 23 (13.1% were conformed as hMPV positive by RT-LAMP, but 18 (10.2% positive by RT-PCR. Conclusion Compared with conventional RT-PCR, the visual hMPV RT-LAMP assay performed well in the aspect of detect time, sensitivity, specificity and visibility. It is anticipated that the RT-LAMP will be used for clinical tests in hospital or field testing during outbreaks and in emergency.

  15. Two key residues in ephrinB3 are critical for its use as an alternative receptor for Nipah virus.

    Directory of Open Access Journals (Sweden)

    Oscar A Negrete

    2006-02-01

    Full Text Available EphrinB2 was recently discovered as a functional receptor for Nipah virus (NiV, a lethal emerging paramyxovirus. Ephrins constitute a class of homologous ligands for the Eph class of receptor tyrosine kinases and exhibit overlapping expression patterns. Thus, we examined whether other ephrins might serve as alternative receptors for NiV. Here, we show that of all known ephrins (ephrinA1-A5 and ephrinB1-B3, only the soluble Fc-fusion proteins of ephrinB3, in addition to ephrinB2, bound to soluble NiV attachment protein G (NiV-G. Soluble NiV-G bound to cell surface ephrinB3 and B2 with subnanomolar affinities (Kd = 0.58 nM and 0.06 nM for ephrinB3 and B2, respectively. Surface plasmon resonance analysis indicated that the relatively lower affinity of NiV-G for ephrinB3 was largely due to a faster off-rate (K(off = 1.94 x 10(-3 s(-1 versus 1.06 x 10(-4 s(-1 for ephrinB3 and B2, respectively. EphrinB3 was sufficient to allow for viral entry of both pseudotype and live NiV. Soluble ephrinB2 and B3 were able to compete for NiV-envelope-mediated viral entry on both ephrinB2- and B3-expressing cells, suggesting that NiV-G interacts with both ephrinB2 and B3 via an overlapping site. Mutational analysis indicated that the Leu-Trp residues in the solvent exposed G-H loop of ephrinB2 and B3 were critical determinants of NiV binding and entry. Indeed, replacement of the Tyr-Met residues in the homologous positions in ephrinB1 with Leu-Trp conferred NiV receptor activity to ephrinB1. Thus, ephrinB3 is a bona fide alternate receptor for NiV entry, and two residues in the G-H loop of the ephrin B-class ligands are critical determinants of NiV receptor activity.

  16. Effects of human metapneumovirus and respiratory syncytial virus antigen insertion in two 3' proximal genome positions of bovine/human parainfluenza virus type 3 on virus replication and immunogenicity

    NARCIS (Netherlands)

    R.S. Tang (Roderick); J.H. Schickli (Jeanne); M. MacPhail (Mia); F. Fernandes (Fiona); L. Bicha (Leenas); J. Spaete (Joshua); R.A.M. Fouchier (Ron); A.D.M.E. Osterhaus (Albert); R. Spaete (Richard); A.A. Haller (Aurelia)

    2003-01-01

    textabstractA live attenuated bovine parainfluenza virus type 3 (PIV3), harboring the fusion (F) and hemagglutinin-neuraminidase (HN) genes of human PIV3, was used as a virus vector to express surface glycoproteins derived from two human pathogens, human metapneumovirus (hMPV) and respiratory syncyt

  17. Deduced amino acid sequence of the small hydrophobic protein of US avian pneumovirus has greater identity with that of human metapneumovirus than those of non-US avian pneumoviruses.

    Science.gov (United States)

    Yunus, Abdul S; Govindarajan, Dhanasekaran; Huang, Zhuhui; Samal, Siba K

    2003-05-01

    We report here the nucleotide and deduced amino acid (aa) sequences of the small hydrophobic (SH) gene of the avian pneumovirus strain Colorado (APV/CO). The SH gene of APV/CO is 628 nucleotides in length from gene-start to gene-end. The longest ORF of the SH gene encoded a protein of 177 aas in length. Comparison of the deduced aa sequence of the SH protein of APV/CO with the corresponding published sequences of other members of genera metapneumovirus showed 28% identity with the newly discovered human metapneumovirus (hMPV), but no discernable identity with the APV subgroup A or B. Collectively, this data supports the hypothesis that: (i) APV/CO is distinct from European APV subgroups and belongs to the novel subgroup APV/C (APV/US); (ii) APV/CO is more closely related to hMPV, a mammalian metapneumovirus, than to either APV subgroup A or B. The SH gene of APV/CO was cloned using a genomic walk strategy which initiated cDNA synthesis from genomic RNA that traversed the genes in the order 3'-M-F-M2-SH-G-5', thus confirming that gene-order of APV/CO conforms in the genus Metapneumovirus. We also provide the sequences of transcription-signals and the M-F, F-M2, M2-SH and SH-G intergenic regions of APV/CO.

  18. 浅谈马来西亚控制尼帕病毒病一些经验%Some Experiences in Control of Nipah Virus Disease in Malaysia

    Institute of Scientific and Technical Information of China (English)

    陈继明; 王志亮

    2003-01-01

    1998-1999年马来西亚暴发尼帕病毒(Nipah virus)病,造成许多人畜伤亡,引起巨大的社会恐慌和经济损失,但疫情得到及时和有效的控制.这是人类成功控制烈性新传染病的范例.本文论述马来西亚在控制此病中在科学研究、行政管理、国际合作等方面值得我们学习和借鉴的一些经验.

  19. A randomized controlled trial of interventions to impede date palm sap contamination by bats to prevent nipah virus transmission in Bangladesh.

    Directory of Open Access Journals (Sweden)

    Salah Uddin Khan

    Full Text Available BACKGROUND: Drinking raw date palm sap is a risk factor for human Nipah virus (NiV infection. Fruit bats, the natural reservoir of NiV, commonly contaminate raw sap with saliva by licking date palm's sap producing surface. We evaluated four types of physical barriers that may prevent bats from contacting sap. METHODS: During 2009, we used a crossover design and randomly selected 20 date palm sap producing trees and observed each tree for 2 nights: one night with a bamboo skirt intervention applied and one night without the intervention. During 2010, we selected 120 trees and randomly assigned four types of interventions to 15 trees each: bamboo, dhoincha (local plant, jute stick and polythene skirts covering the shaved part, sap stream, tap and collection pot. We enrolled the remaining 60 trees as controls. We used motion sensor activated infrared cameras to examine bat contact with sap. RESULTS: During 2009 bats contacted date palm sap in 85% of observation nights when no intervention was used compared with 35% of nights when the intervention was used [p<0.001]. Bats were able to contact the sap when the skirt did not entirely cover the sap producing surface. Therefore, in 2010 we requested the sap harvesters to use larger skirts. During 2010 bats contacted date palm sap [2% vs. 83%, p<0.001] less frequently in trees protected with skirts compared to control trees. No bats contacted sap in trees with bamboo (p<0.001 compared to control, dhoincha skirt (p<0.001 or polythene covering (p<0.001, but bats did contact sap during one night (7% with the jute stick skirt (p<0.001. CONCLUSION: Bamboo, dhoincha, jute stick and polythene skirts covering the sap producing areas of a tree effectively prevented bat-sap contact. Community interventions should promote applying these skirts to prevent occasional Nipah spillovers to human.

  20. Prokaryotic expression of Nipah virus N gene%尼帕病毒N基因的原核表达

    Institute of Scientific and Technical Information of China (English)

    梁栋; 涂长春; 石清明; 张超雄; 陈锚锚; 邓成玉; 范泉水

    2015-01-01

    Objective To explore the possibility of Nipah virus ( NiV) N protein as an antigen to detect the presence of NiV by using the prokaryotic expression of NiV N gene .Methods pET-28a (+) was used as an expression vector in order to construct the recombinant plasmid of pET-NivN, which was induced by IPTG to express the protein; and then, Western blot method was used to detect the prokaryotic expression of NivN protein .Results By means of PCR , enzyme digestion and ligation reaction , the recombinant plasmid of pET-NivN was constructed successfully , with a size of 1596 bp;IPTG was used to induce the recombinant plasmid of pET-NivN to express N protein in the host bacteria .SDS-PAGE results showed that 55 kD has a specific protein band with the expected protein size; according to the Bandscan software scanning and analysis , the amount of recombinant protein was 12% of the total bacteria protein .Western blot assay showed that the band of 55 kD has a good color , and this proved that it was just the protein to be obtained in the experiment .Conclusion The prokaryotic expression system can express N protein of NiV successfully , and this lays solid foundation for further antibody preparation , activity evaluation , and research application .%目的:利用尼帕病毒(NiV)的N基因进行原核表达,探讨NiV N蛋白作为检测NiV存在的抗原的可能性。方法利用pET-28a(+)作为表达载体,构建pET-NiV N重组质粒,通过IPTG诱导使其表达蛋白,并用Western blot方法进行原核表达NiV N蛋白的检测。结果通过PCR、酶切和连接反应,成功构建N蛋白的pET-NiV N重组质粒,大小为1596 bp;利用IPTG诱导pET-NiV N重组质粒在宿主菌中表达N蛋白,SDS-PAGE结果显示,55 kD有特异蛋白条带,与蛋白预计大小相符,经Bandscan软件扫描分析,重组蛋白量占菌体总蛋白量的12%;Western blot法检测显示,55 kD的条带显色良好,证明是本实验所需要得到的

  1. Genetic variability of attachment (G and Fusion (F protein genes of human metapneumovirus strains circulating during 2006-2009 in Kolkata, Eastern India

    Directory of Open Access Journals (Sweden)

    Chawla-Sarkar Mamta

    2011-02-01

    Full Text Available Abstract Background Human metapneumovirus (hMPV is associated with the acute respiratory tract infection (ARTI in all the age groups. However, there is limited information on prevalence and genetic diversity of human metapneumovirus (hMPV strains circulating in India. Objective To study prevalence and genomic diversity of hMPV strains among ARTI patients reporting in outpatient departments of hospitals in Kolkata, Eastern India. Methods Nasal and/or throat swabs from 2309 patients during January 2006 to December 2009, were screened for the presence of hMPV by RT-PCR of nucleocapsid (N gene. The G and F genes of representative hMPV positive samples were sequenced. Results 118 of 2309 (5.11% clinical samples were positive for hMPV. The majority (≈80% of the positive cases were detected during July−November all through the study period. Genetic analysis revealed that 77% strains belong to A2 subgroup whereas rest clustered in B1 subgroup. G sequences showed higher diversity at the nucleotide and amino acid level. In contrast, less than 10% variation was observed in F gene of representative strains of all four years. Sequence analysis also revealed changes in the position of stop codon in G protein, which resulted in variable length (217-231 aa polypeptides. Conclusion The study suggests that approximately 5% of ARTI in the region were caused by hMPV. This is the first report on the genetic variability of G and F gene of hMPV strains from India which clearly shows that the G protein of hMPV is continuously evolving. Though the study partially fulfills lacunae of information, further studies from other regions are necessary for better understanding of prevalence, epidemiology and virus evolution in Indian subcontinent.

  2. West Africa

    Science.gov (United States)

    2002-01-01

    With its vast expanses of sand, framed by mountain ranges and exposed rock, northwestern Africa makes a pretty picture when viewed from above. This image was acquired by the Moderate-resolution Imaging Spectroradiometer (MODIS), flying aboard NASA's Terra spacecraft. The Canary Islands can be seen on the left side of the image just off Africa's Atlantic shore. The light brown expanse running through the northern two thirds of the image is the Sahara Desert. The desert runs up against the dark brown Haut Atlas mountain range of Morocco in the northwest, the Atlantic Ocean to the west and the semi-arid (light brown pixels) Sahelian region in the South. The Sahara, however, isn't staying put. Since the 1960s, the desert has been expanding into the Sahelian region at a rate of up to 6 kilometers per year. In the 1980s this desert expansion, combined with over cultivation of the Sahel, caused a major famine across west Africa. Over the summer months, strong winds pick up sands from the Sahara and blow them across the Atlantic as far west as North America, causing air pollution in Miami and damaging coral reefs in the Bahamas and the Florida Keys. The white outlines on the map represent country borders. Starting at the top-most portion of the map and working clockwise, the countries shown are Morocco, Western Sahara, Mauritania, Senegal, Mali, Burkina Fasso, Nigeria, Mali (again), and Algeria. Image by Reto Stockli, Robert Simmon, and Brian Montgomery, NASA Earth Observatory, based on data from MODIS

  3. Rational Design of Human Metapneumovirus Live Attenuated Vaccine Candidates by Inhibiting Viral mRNA Cap Methyltransferase

    Science.gov (United States)

    Zhang, Yu; Wei, Yongwei; Zhang, Xiaodong; Cai, Hui; Niewiesk, Stefan

    2014-01-01

    ABSTRACT The paramyxoviruses human respiratory syncytial virus (hRSV), human metapneumovirus (hMPV), and human parainfluenza virus type 3 (hPIV3) are responsible for the majority of pediatric respiratory diseases and inflict significant economic loss, health care costs, and emotional burdens. Despite major efforts, there are no vaccines available for these viruses. The conserved region VI (CR VI) of the large (L) polymerase proteins of paramyxoviruses catalyzes methyltransferase (MTase) activities that typically methylate viral mRNAs at positions guanine N-7 (G-N-7) and ribose 2′-O. In this study, we generated a panel of recombinant hMPVs carrying mutations in the S-adenosylmethionine (SAM) binding site in CR VI of L protein. These recombinant viruses were specifically defective in ribose 2′-O methylation but not G-N-7 methylation and were genetically stable and highly attenuated in cell culture and viral replication in the upper and lower respiratory tracts of cotton rats. Importantly, vaccination of cotton rats with these recombinant hMPVs (rhMPVs) with defective MTases triggered a high level of neutralizing antibody, and the rats were completely protected from challenge with wild-type rhMPV. Collectively, our results indicate that (i) amino acid residues in the SAM binding site in the hMPV L protein are essential for 2′-O methylation and (ii) inhibition of mRNA cap MTase can serve as a novel target to rationally design live attenuated vaccines for hMPV and perhaps other paramyxoviruses, such as hRSV and hPIV3. IMPORTANCE Human paramyxoviruses, including hRSV, hMPV, and hPIV3, cause the majority of acute upper and lower respiratory tract infections in humans, particularly in infants, children, the elderly, and immunocompromised individuals. Currently, there is no licensed vaccine available. A formalin-inactivated vaccine is not suitable for these viruses because it causes enhanced lung damage upon reinfection with the same virus. A live attenuated vaccine

  4. Contribution of Human Lung Parenchyma and Leukocyte Influx to Oxidative Stress and Immune System-Mediated Pathology following Nipah Virus Infection.

    Science.gov (United States)

    Escaffre, Olivier; Saito, Tais B; Juelich, Terry L; Ikegami, Tetsuro; Smith, Jennifer K; Perez, David D; Atkins, Colm; Levine, Corri B; Huante, Matthew B; Nusbaum, Rebecca J; Endsley, Janice J; Freiberg, Alexander N; Rockx, Barry

    2017-08-01

    Nipah virus (NiV) is a zoonotic emerging paramyxovirus that can cause fatal respiratory illness or encephalitis in humans. Despite many efforts, the molecular mechanisms of NiV-induced acute lung injury (ALI) remain unclear. We previously showed that NiV replicates to high titers in human lung grafts in NOD-SCID/γ mice, resulting in a robust inflammatory response. Interestingly, these mice can undergo human immune system reconstitution by the bone marrow, liver, and thymus (BLT) reconstitution method, in addition to lung tissue engraftment, giving altogether a realistic model to study human respiratory viral infections. Here, we characterized NiV Bangladesh strain (NiV-B) infection of human lung grafts from human immune system-reconstituted mice in order to identify the overall effect of immune cells on NiV pathogenesis of the lung. We show that NiV-B replicated to high titers in human lung grafts and caused similar cytopathic effects irrespective of the presence of human leukocytes in mice. However, the human immune system interfered with virus spread across lung grafts, responded to infection by leukocyte migration to small airways and alveoli of the lung grafts, and accelerated oxidative stress in lung grafts. In addition, the presence of human leukocytes increased the expression of cytokines and chemokines that regulate inflammatory influx to sites of infection and tissue damage. These results advance our understanding of how the immune system limits NiV dissemination and contributes to ALI and inform efforts to identify therapeutic targets.IMPORTANCE Nipah virus (NiV) is an emerging paramyxovirus that can cause a lethal respiratory and neurological disease in humans. Only limited data are available on NiV pathogenesis in the human lung, and the relative contribution of the innate immune response and NiV to acute lung injury (ALI) is still unknown. Using human lung grafts in a human immune system-reconstituted mouse model, we showed that the NiV Bangladesh

  5. Prediction of Epitope-Based Peptides for the Utility of Vaccine Development from Fusion and Glycoprotein of Nipah Virus Using In Silico Approach

    Directory of Open Access Journals (Sweden)

    M. Sadman Sakib

    2014-01-01

    Full Text Available This study aims to design epitope-based peptides for the utility of vaccine development by targeting glycoprotein G and envelope protein F of Nipah virus (NiV that, respectively, facilitate attachment and fusion of NiV with host cells. Using various databases and tools, immune parameters of conserved sequence(s from G and F proteins of different isolates of NiV were tested to predict probable epitope(s. Binding analyses of the peptides with MHC class-I and class-II molecules, epitope conservancy, population coverage, and linear B cell epitope prediction were analyzed. Predicted peptides interacted with seven or more MHC alleles and illustrated population coverage of more than 99% and 95%, for G and F proteins, respectively. The predicted class-I nonamers, SLIDTSSTI and EWISIVPNF, superimposed on the putative decameric B cell epitopes, were also identified as core sequences of the most probable class-II 15-mer peptides GPKVSLIDTSSTITI and EWISIVPNFILVRNT. These peptides were further validated for their binding to specific HLA alleles using in silico docking technique. Our in silico analysis suggested that the predicted epitopes, either GPKVSLIDTSSTITI or EWISIVPNFILVRNT, could be a better choice as universal vaccine component against NiV irrespective of different isolates which may elicit both humoral and cell-mediated immunity.

  6. Second generation of pseudotype-based serum neutralization assay for Nipah virus antibodies: sensitive and high-throughput analysis utilizing secreted alkaline phosphatase.

    Science.gov (United States)

    Kaku, Yoshihiro; Noguchi, Akira; Marsh, Glenn A; Barr, Jennifer A; Okutani, Akiko; Hotta, Kozue; Bazartseren, Boldbaatar; Fukushi, Shuetsu; Broder, Christopher C; Yamada, Akio; Inoue, Satoshi; Wang, Lin-Fa

    2012-01-01

    Nipah virus (NiV), Paramyxoviridae, Henipavirus, is classified as a biosafety level (BSL) 4 pathogen, along with the closely related Hendra virus (HeV). A novel serum neutralization test was developed for measuring NiV neutralizing antibodies under BSL2 conditions using a recombinant vesicular stomatitis virus (VSV) expressing secreted alkaline phosphatase (SEAP) and pseudotyped with NiV F/G proteins (VSV-NiV-SEAP). A unique characteristic of this novel assay is the ability to obtain neutralization titers by measuring SEAP activity in supernatant using a common ELISA plate reader. This confers a remarkable advantage over the first generation of NiV-pseudotypes expressing green fluorescent protein or luciferase, which require expensive and specific measuring equipment. Using panels of NiV- and HeV-specific sera from various species, the VSV-NiV-SEAP assay demonstrated neutralizing antibody status (positive/negative) consistent with that obtained by conventional live NiV test, and gave higher antibody titers than the latter. Additionally, when screening sixty-six fruit bat sera at one dilution, the VSV-NiV-SEAP assay produced identical results to the live NiV test and only required a very small amount (2μl) of sera. The results suggest that this novel VSV-NiV-SEAP assay is safe, useful for high-throughput screening of sera using an ELISA plate reader, and has high sensitivity and specificity.

  7. Piloting the use of indigenous methods to prevent Nipah virus infection by interrupting bats' access to date palm sap in Bangladesh.

    Science.gov (United States)

    Nahar, Nazmun; Mondal, Utpal Kumar; Sultana, Rebeca; Hossain, M Jahangir; Khan, M Salah Uddin; Gurley, Emily S; Oliveras, Elizabeth; Luby, Stephen P

    2013-09-01

    People in Bangladesh frequently drink fresh date palm sap. Fruit bats (Pteropus giganteus) also drink raw sap and may contaminate the sap by shedding Nipah virus through saliva and urine. In a previous study we identified two indigenous methods to prevent bats accessing the sap, bamboo skirts and lime (calcium carbonate). We conducted a pilot study to assess the acceptability of these two methods among sap harvesters. We used interactive community meetings and group discussions to encourage all the sap harvesters (n = 12) from a village to use either bamboo skirts or lime smear that some of them (n = 4) prepared and applied. We measured the preparation and application time and calculated the cost of bamboo skirts. We conducted interviews after the use of each method. The sap harvesters found skirts effective in preventing bats from accessing sap. They were sceptical that lime would be effective as the lime was washed away by the sap flow. Preparation of the skirt took ∼105 min. The application of each method took ∼1 min. The cost of the bamboo skirt is minimal because bamboo is widely available and they made the skirts with pieces of used bamboo. The bamboo skirt method appeared practical and affordable to the sap harvesters. Further studies should explore its ability to prevent bats from accessing date palm sap and assess if its use produces more or better quality sap, which would provide further incentives to make it more acceptable for its regular use.

  8. Nipah Virus Transmission from Bats to Humans Associated with Drinking Traditional Liquor Made from Date Palm Sap, Bangladesh, 2011-2014.

    Science.gov (United States)

    Islam, M Saiful; Sazzad, Hossain M S; Satter, Syed Moinuddin; Sultana, Sharmin; Hossain, M Jahangir; Hasan, Murshid; Rahman, Mahmudur; Campbell, Shelley; Cannon, Deborah L; Ströher, Ute; Daszak, Peter; Luby, Stephen P; Gurley, Emily S

    2016-04-01

    Nipah virus (NiV) is a paramyxovirus, and Pteropus spp. bats are the natural reservoir. From December 2010 through March 2014, hospital-based encephalitis surveillance in Bangladesh identified 18 clusters of NiV infection. The source of infection for case-patients in 3 clusters in 2 districts was unknown. A team of epidemiologists and anthropologists investigated these 3 clusters comprising 14 case-patients, 8 of whom died. Among the 14 case-patients, 8 drank fermented date palm sap (tari) regularly before their illness, and 6 provided care to a person infected with NiV. The process of preparing date palm trees for tari production was similar to the process of collecting date palm sap for fresh consumption. Bat excreta was reportedly found inside pots used to make tari. These findings suggest that drinking tari is a potential pathway of NiV transmission. Interventions that prevent bat access to date palm sap might prevent tari-associated NiV infection.

  9. The Nipah virus disease risk early warning and prevention and control measures in our country port%我国口岸尼帕病毒病风险预警及防控措施探讨

    Institute of Scientific and Technical Information of China (English)

    吴岳

    2015-01-01

    目的:尼帕病毒病是由尼帕病毒引起的主要侵害中枢神经系统和呼吸系统的急性高度致死性人畜共患传染病。目前该病在东南亚国家和地区流行蔓延,我国尚未有此病的报道,但是一旦传入将严重威胁人民生命安全和养殖业发展。方法为防范疫病传入,本文根据尼帕病毒的流行病学特性及国家质量监督检验检疫总局风险管理规定,对口岸尼帕病毒病的风险预警的重要性及防控措施进行初步的探讨。结果东南亚国家和地区与我国地理位置接近,同时我国是生猪养殖大国,从生物学角度来看我国具有疫病传入风险。结论根据风险等级进行管理,兽医主管部门和口岸检疫部门加强风险预警和管理,严格防控尼帕病毒进入我国境内。%Objective The Nipah virus disease is an acute highly fatal zoonotic infectious disease caused by Nipah virus which mainly invades the central nervous system and respiratory system. At present, the disease is popular in Southeast Asia countries and regions, and our country has not been reported with the disease. But once it is intro-duced to China, it will be a serious threat to the safety of people's life and the development cultivation industry. Methods To prevent the disease from introducing to our country, based on the epidemiological characteristics of Nipah virus and risk supervision regulations of AQSIQ, this article carries out a preliminary discussion on the im-portance of Nipah virus disease's early warning and prevention and its control measures. Results Southeast Asia countries and regions are geographically close to China. And at the same time, pig farming is a very important in-dustry in China, the risk of Nipah virus disease infection biologically exists. Conclusion The veterinary authori-ties and port quarantine departments should strengthen early warning and risk management, and carry out strict pre-vention and control of Nipah

  10. Avian metapneumovirus RT-nested-PCR: a novel false positive reducing inactivated control virus with potential applications to other RNA viruses and real time methods.

    Science.gov (United States)

    Falchieri, Marco; Brown, Paul A; Catelli, Elena; Naylor, Clive J

    2012-12-01

    Using reverse genetics, an avian metapneumovirus (AMPV) was modified for use as a positive control for validating all stages of a popular established RT-nested PCR, used in the detection of the two major AMPV subtypes (A and B). Resultant amplicons were of increased size and clearly distinguishable from those arising from unmodified virus, thus allowing false positive bands, due to control virus contamination of test samples, to be identified readily. Absorption of the control virus onto filter paper and subsequent microwave irradiation removed all infectivity while its function as an efficient RT-nested-PCR template was unaffected. Identical amplicons were produced after storage for one year. The modified virus is likely to have application as an internal standard as well as in real time methods. Additions to AMPV of RNA from other RNA viruses, including hazardous examples such HIV and influenza, are likely to yield similar safe RT-PCR controls. Copyright © 2012 Elsevier B.V. All rights reserved.

  11. Development of a multiplex RT-PCR for simultaneous diagnosis of human metapneumovirus (HMPV) and human respiratory syncytial virus (HRSV) from clinical specimens.

    Science.gov (United States)

    Dayakar, Seetha; Pillai, Heera R; Thulasi, Vineetha P; Nair, Radhakrishnan R

    2016-12-01

    Human metapneumovirus (HMPV) and human respiratory syncytial virus (HRSV) are ubiquitous respiratory viral pathogens. They belong to the family Paramyxoviridae (subfamily Pneumovirinae) and is responsible for acute respiratory tract infections in children, elderly and immunocompromised patients. We designed and tested a multiplex reverse transcriptase polymerase chain reaction (mRT-PCR) as a cost-effective alternative to real-time PCR and cell culture based detection for HMPV and HRSV. The newly developed PCR was used to screen nasal/throat swab samples from 356 patients with suspected acute respiratory infection attending the Government Medical College, Thiruvananthapuram, Kerala, India. The method was compared with a commercially available kit employing real time PCR, for its sensitivity and specificity. 53 (14.9 %) samples were positive for at least one tested pathogen by mRT-PCR. All except one among the positive samples showed similar pathogen profile when tested using real time PCR. 8 (15.1 %) among these 53 were positive for HRSVA, 33 (62.3 %) positive for HRSVB and 12 (22.6 %) were positive for HMPV. 17 (32.7 %) samples showed co-infections in them. Sensitivity and specificity of the mRT-PCR was comparable to that of the commercial kit. Our findings indicate that this newly developed mRT-PCR can be used as a cost-effective alternative for laboratory diagnosis of HMPV/HRSV infection and will significantly reduce diagnostic costs for these viruses in clinical settings.

  12. Genetic diversity, seasonality and transmission network of human metapneumovirus: identification of a unique sub-lineage of the fusion and attachment genes

    Science.gov (United States)

    Chow, Wei Zhen; Chan, Yoke Fun; Oong, Xiang Yong; Ng, Liang Jie; Nor’E, Siti Sarah; Ng, Kim Tien; Chan, Kok Gan; Hanafi, Nik Sherina; Pang, Yong Kek; Kamarulzaman, Adeeba; Tee, Kok Keng

    2016-01-01

    Human metapneumovirus (HMPV) is an important viral respiratory pathogen worldwide. Current knowledge regarding the genetic diversity, seasonality and transmission dynamics of HMPV among adults and children living in tropical climate remains limited. HMPV prevailed at 2.2% (n = 86/3,935) among individuals presented with acute respiratory tract infections in Kuala Lumpur, Malaysia between 2012 and 2014. Seasonal peaks were observed during the northeast monsoon season (November–April) and correlated with higher relative humidity and number of rainy days (P < 0.05). Phylogenetic analysis of the fusion and attachment genes identified the co-circulation of three known HMPV sub-lineages, A2b and B1 (30.2% each, 26/86) and B2 (20.9%, 18/86), with genotype shift from sub-lineage B1 to A2b observed in 2013. Interestingly, a previously unrecognized sub-lineage of A2 was identified in 18.6% (16/86) of the population. Using a custom script for network construction based on the TN93 pairwise genetic distance, we identified up to nine HMPV transmission clusters circulating as multiple sub-epidemics. Although no apparent major outbreak was observed, the increased frequency of transmission clusters (dyads) during seasonal peaks suggests the potential roles of transmission clusters in driving the spread of HMPV. Our findings provide essential information for therapeutic research, prevention strategies, and disease outbreak monitoring of HMPV. PMID:27279080

  13. Receptor-Targeted Nipah Virus Glycoproteins Improve Cell-Type Selective Gene Delivery and Reveal a Preference for Membrane-Proximal Cell Attachment.

    Directory of Open Access Journals (Sweden)

    Ruben R Bender

    2016-06-01

    Full Text Available Receptor-targeted lentiviral vectors (LVs can be an effective tool for selective transfer of genes into distinct cell types of choice. Moreover, they can be used to determine the molecular properties that cell surface proteins must fulfill to act as receptors for viral glycoproteins. Here we show that LVs pseudotyped with receptor-targeted Nipah virus (NiV glycoproteins effectively enter into cells when they use cell surface proteins as receptors that bring them closely enough to the cell membrane (less than 100 Å distance. Then, they were flexible in receptor usage as demonstrated by successful targeting of EpCAM, CD20, and CD8, and as selective as LVs pseudotyped with receptor-targeted measles virus (MV glycoproteins, the current standard for cell-type specific gene delivery. Remarkably, NiV-LVs could be produced at up to two orders of magnitude higher titers compared to their MV-based counterparts and were at least 10,000-fold less effectively neutralized than MV glycoprotein pseudotyped LVs by pooled human intravenous immunoglobulin. An important finding for NiV-LVs targeted to Her2/neu was an about 100-fold higher gene transfer activity when particles were targeted to membrane-proximal regions as compared to particles binding to a more membrane-distal epitope. Likewise, the low gene transfer activity mediated by NiV-LV particles bound to the membrane distal domains of CD117 or the glutamate receptor subunit 4 (GluA4 was substantially enhanced by reducing receptor size to below 100 Å. Overall, the data suggest that the NiV glycoproteins are optimally suited for cell-type specific gene delivery with LVs and, in addition, for the first time define which parts of a cell surface protein should be targeted to achieve optimal gene transfer rates with receptor-targeted LVs.

  14. What's West Nile Virus?

    Science.gov (United States)

    ... About Puberty Train Your Temper What's West Nile Virus? KidsHealth > For Kids > What's West Nile Virus? Print A A A en español ¿Qué es el Virus del Nilo Occidental? What exactly is the West ...

  15. Humane metapneumovirus (HMPV) associated pulmonary infections in immunocompromised adults—Initial CT findings, disease course and comparison to respiratory-syncytial-virus (RSV) induced pulmonary infections

    Energy Technology Data Exchange (ETDEWEB)

    Syha, R., E-mail: roland.syha@med.uni-tuebingen.de [Department of Diagnostic Radiology, Eberhard-Karls-University, Hoppe-Seyler-Str.3, 72076 Tübingen (Germany); Beck, R. [Institute of Medical Virology, Eberhard-Karls-University, Elfriede-Authorn-Str. 6, 72076 Tübingen (Germany); Hetzel, J. [Department of Medical Oncology and Hematology, Eberhard-Karls-University, Otfried-Müller-Str. 10, 72070 Tübingen (Germany); Ketelsen, D.; Grosse, U.; Springer, F.; Horger, M. [Department of Diagnostic Radiology, Eberhard-Karls-University, Hoppe-Seyler-Str.3, 72076 Tübingen (Germany)

    2012-12-15

    Aim: To describe computed tomography (CT)-imaging findings in human metapneumovirus (HMPV)-related pulmonary infection as well as their temporal course and to analyze resemblances/differences to pulmonary infection induced by the closely related respiratory-syncytial-virus (RSV) in immunocompromised patients. Materials and methods: Chest-CT-scans of 10 HMPV PCR-positive patients experiencing pulmonary symptoms were evaluated retrospectively with respect to imaging findings and their distribution and results were then compared with data acquired in 13 patients with RSV pulmonary infection. Subsequently, we analyzed the course of chest-findings in HMPV patients. Results: In HMPV, 8/10 patients showed asymmetric pulmonary findings, whereas 13/13 patients with RSV-pneumonia presented more symmetrical bilateral pulmonary infiltrates. Image analysis yielded in HMPV patients following results: ground-glass-opacity (GGO) (n = 6), parenchymal airspace consolidations (n = 5), ill-defined nodular-like centrilobular opacities (n = 9), bronchial wall thickening (n = 8). In comparison, results in RSV patients were: GGO (n = 10), parenchymal airspace consolidations (n = 9), ill-defined nodular-like centrilobular opacities (n = 10), bronchial wall thickening (n = 4). In the course of the disease, signs of acute HMPV interstitial pneumonia regressed transforming temporarily in part into findings compatible with bronchitis/bronchiolitis. Conclusions: Early chest-CT findings in patients with HMPV-related pulmonary symptoms are compatible with asymmetric acute interstitial pneumonia accompanied by signs of bronchitis; the former transforming with time into bronchitis and bronchiolitis before they resolve. On the contrary, RSV-induced pulmonary infection exhibits mainly symmetric acute interstitial pneumonia.

  16. Low CCR7-mediated migration of human monocyte derived dendritic cells in response to human respiratory syncytial virus and human metapneumovirus.

    Directory of Open Access Journals (Sweden)

    Cyril Le Nouën

    2011-06-01

    Full Text Available Human respiratory syncytial virus (HRSV and, to a lesser extent, human metapneumovirus (HMPV and human parainfluenza virus type 3 (HPIV3, can re-infect symptomatically throughout life without significant antigenic change, suggestive of incomplete or short-lived immunity. In contrast, re-infection by influenza A virus (IAV largely depends on antigenic change, suggestive of more complete immunity. Antigen presentation by dendritic cells (DC is critical in initiating the adaptive immune response. Antigen uptake by DC induces maturational changes that include decreased expression of the chemokine receptors CCR1, CCR2, and CCR5 that maintain DC residence in peripheral tissues, and increased expression of CCR7 that mediates the migration of antigen-bearing DC to lymphatic tissue. We stimulated human monocyte-derived DC (MDDC with virus and found that, in contrast to HPIV3 and IAV, HMPV and HRSV did not efficiently decrease CCR1, 2, and 5 expression, and did not efficiently increase CCR7 expression. Consistent with the differences in CCR7 mRNA and protein expression, MDDC stimulated with HRSV or HMPV migrated less efficiently to the CCR7 ligand CCL19 than did IAV-stimulated MDDC. Using GFP-expressing recombinant virus, we showed that the subpopulation of MDDC that was robustly infected with HRSV was particularly inefficient in chemokine receptor modulation. HMPV- or HRSV-stimulated MDDC responded to secondary stimulation with bacterial lipopolysaccharide or with a cocktail of proinflammatory cytokines by increasing CCR7 and decreasing CCR1, 2 and 5 expression, and by more efficient migration to CCL19, suggesting that HMPV and HRSV suboptimally stimulate rather than irreversibly inhibit MDDC migration. This also suggests that the low concentration of proinflammatory cytokines released from HRSV- and HMPV-stimulated MDDC is partly responsible for the low CCR7-mediated migration. We propose that inefficient migration of HRSV- and HMPV-stimulated DC to

  17. Subgroup C avian metapneumovirus (MPV) and the recently isolated human MPV exhibit a common organization but have extensive sequence divergence in their putative SH and G genes.

    Science.gov (United States)

    Toquin, D; de Boisseson, C; Beven, V; Senne, D A; Eterradossi, N

    2003-08-01

    The genes encoding the putative small hydrophobic (SH), attachment (G) and polymerase (L) proteins of the Colorado isolate of subgroup C avian pneumovirus (APV) were entirely or partially sequenced. They all included metapneumovirus (MPV)-like gene start and gene end sequences. The deduced Colorado SH protein shared 26.9 and 21.7 % aa identity with its counterpart in human MPV (hMPV) and APV subgroup A, respectively, but its only significant aa similarities were to hMPV. Conserved features included a common hydrophobicity profile with an unique transmembrane domain and the conservation of most extracellular cysteine residues. The Colorado putative G gene encoded several ORFs, the longer of which encoded a 252 aa long type II glycoprotein with aa similarities to hMPV G only (20.6 % overall aa identity with seven conserved N-terminal residues). The putative Colorado G protein shared, at best, 21.0 % aa identity with its counterparts in the other APV subgroups and did not contain the extracellular cysteine residues and short aa stretch highly conserved in other APVs. The N-terminal end of the Colorado L protein exhibited 73.6 and 54.9 % aa identity with hMPV and APV subgroup A, respectively, with four aa blocks highly conserved among Pneumovirus: Phylogenetic analysis performed on the nt sequences confirmed that the L sequences from MPVs were genetically related, whereas analysis of the G sequences revealed that among MPVs, only APV subgroups A, B and D clustered together, independently of both the Colorado isolate and hMPV, which shared weak genetic relatedness at the G gene level.

  18. Effects of human respiratory syncytial virus, metapneumovirus, parainfluenza virus 3 and influenza virus on CD4+ T cell activation by dendritic cells.

    Directory of Open Access Journals (Sweden)

    Cyril Le Nouën

    Full Text Available BACKGROUND: Human respiratory syncytial virus (HRSV, and to a lesser extent human metapneumovirus (HMPV and human parainfluenza virus type 3 (HPIV3, re-infect symptomatically throughout life without antigenic change, suggestive of incomplete immunity. One causative factor is thought to be viral interference with dendritic cell (DC-mediated stimulation of CD4+ T cells. METHODOLOGY, PRINCIPAL FINDINGS: We infected human monocyte-derived DC with purified HRSV, HMPV, HPIV3, or influenza A virus (IAV and compared their ability to induce activation and proliferation of autologous CD4+ T cells in vitro. IAV was included because symptomatic re-infection without antigenic change is less frequent, suggesting that immune protection is more complete and durable. We examined virus-specific memory responses and superantigen-induced responses by multiparameter flow cytometry. Live virus was more stimulatory than inactivated virus in inducing DC-mediated proliferation of virus-specific memory CD4+ T cells, suggesting a lack of strong suppression by live virus. There were trends of increasing proliferation in the order: HMPV

  19. Call From China West

    Institute of Scientific and Technical Information of China (English)

    Yang Wei; Guo Jin

    2008-01-01

    @@ The 12th East-West China Cooperation and Investment and Trade Fair was held from April 5 to 8 at the International Conference and Exhibition Center in Qu-jiang,Xi'an.Shaanxi province,in the west of China.

  20. US west coast

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Aerial surveys are conducted along the US west coast to determine distribution and abundance of endangered leatherback turtles (Dermochelys coriacea), loggerhead...

  1. WEST Physics Basis

    Science.gov (United States)

    Bourdelle, C.; Artaud, J. F.; Basiuk, V.; Bécoulet, M.; Brémond, S.; Bucalossi, J.; Bufferand, H.; Ciraolo, G.; Colas, L.; Corre, Y.; Courtois, X.; Decker, J.; Delpech, L.; Devynck, P.; Dif-Pradalier, G.; Doerner, R. P.; Douai, D.; Dumont, R.; Ekedahl, A.; Fedorczak, N.; Fenzi, C.; Firdaouss, M.; Garcia, J.; Ghendrih, P.; Gil, C.; Giruzzi, G.; Goniche, M.; Grisolia, C.; Grosman, A.; Guilhem, D.; Guirlet, R.; Gunn, J.; Hennequin, P.; Hillairet, J.; Hoang, T.; Imbeaux, F.; Ivanova-Stanik, I.; Joffrin, E.; Kallenbach, A.; Linke, J.; Loarer, T.; Lotte, P.; Maget, P.; Marandet, Y.; Mayoral, M. L.; Meyer, O.; Missirlian, M.; Mollard, P.; Monier-Garbet, P.; Moreau, P.; Nardon, E.; Pégourié, B.; Peysson, Y.; Sabot, R.; Saint-Laurent, F.; Schneider, M.; Travère, J. M.; Tsitrone, E.; Vartanian, S.; Vermare, L.; Yoshida, M.; Zagorski, R.; Contributors, JET

    2015-06-01

    With WEST (Tungsten Environment in Steady State Tokamak) (Bucalossi et al 2014 Fusion Eng. Des. 89 907-12), the Tore Supra facility and team expertise (Dumont et al 2014 Plasma Phys. Control. Fusion 56 075020) is used to pave the way towards ITER divertor procurement and operation. It consists in implementing a divertor configuration and installing ITER-like actively cooled tungsten monoblocks in the Tore Supra tokamak, taking full benefit of its unique long-pulse capability. WEST is a user facility platform, open to all ITER partners. This paper describes the physics basis of WEST: the estimated heat flux on the divertor target, the planned heating schemes, the expected behaviour of the L-H threshold and of the pedestal and the potential W sources. A series of operating scenarios has been modelled, showing that ITER-relevant heat fluxes on the divertor can be achieved in WEST long pulse H-mode plasmas.

  2. Purge at West Valley

    Science.gov (United States)

    Mack, Warren

    1977-01-01

    Tells how the adviser of the student newspaper at West Valley College (Saratoga, California) was dismissed after the newspaper published stories based on investigations into alleged wrongdoings by administration members. (GW)

  3. Dracaena in West Africa

    NARCIS (Netherlands)

    Bos, J.J.

    1984-01-01

    This taxonomic revision of the genus Dracaena L. (Liliaceae) in West Africa is another contribution towards a monograph on this group.Short general chapters contain historical, phytogeographical, morphological and phylogenetic observations. The taxonomic treatment contains a revised genus descriptio

  4. Eastern Culture Gone West

    Institute of Scientific and Technical Information of China (English)

    ZHANGHONG

    2005-01-01

    THE implication of one of British 19th century writer Rudyard Kipling's most famous quotations: “East is East,West is West and never the twain shall meet” is endorsed by contemporary scholar Dr Samuel Huntington in his work The Clash of Civilizations, in which he asserts that future wars will not be between individual states and political unions but between differing civilizations.

  5. Islam and the West

    Directory of Open Access Journals (Sweden)

    Mohd. Kamal Hassan

    1997-06-01

    Full Text Available The scientific and technological developments during the 18th and' the 19th centuries ensured material progress of the West, as well as emergence of the West as the dominating power which colonized the rest of the world. During the post-colonial phase, Islam emerged as a revitalized sociopolitical force. This has been mistaken as a threat by the West, and Islam has been portrayed as the "new enemy after the demise of communism. This is partly an effort to establish a Western identity, which is disintegrating due to lack of a challenge; and partly a reflection of the failure of Muslims to realize the social and ethical ideals of Islam.

  6. 禽偏肺病毒地高辛核酸探针的制备与应用%Preparation and application of digoxigenin-labeled nucleic acid probe for detection of avian metapneumovirus

    Institute of Scientific and Technical Information of China (English)

    陈琳; 刁有祥; 鞠小军; 慕榕; 郑新颖; 刘霞; 孙静; 吴海洋

    2012-01-01

    根据GenBank中已经发表的B亚型禽偏肺病毒F基因的保守序列设计并合成1对引物,利用RT—PCR扩增出1条与目的片段大小一致的725bp基因片段,回收、纯化PCR产物,用地高辛标记,制备出地高辛标记的aMPV核酸探针。特异性检测结果表明,该探针能与aMPV核酸发生特异性杂交,而与H9N2亚型AIV、NDV、IBV、ORT和E.coil的核酸杂交反应均为阴性;敏感性检测结果表明,该探针对aMPV的最低检出量为5Pg。应用制备的探针对山东省不同地区的605份商品肉鸡和122份商品肉鸭进行了核酸探针检测,阳性检出率分别为36.59%和34.51%。本试验制备的aMPV地高辛探针特异性强、敏感性好,对样品的检测结果表明山东省部分地区的商品肉鸡、肉鸭中普遍存在aMPV感染。%According to the genomic sequences of F gene of avian metapneumovirus (aMPV) published in GenBank,a pair of primers was designed for amplifying the 725 hp fragment in RT-PCR experiments. Then the PCR product was labeled with DIG as cDNA probe for detection of aMPV. The hybridization assay of specificity showed that the DNA of aMPV were positive,but other nucleotide extracted from AIV, NDV, IBV, ORT and E. coil were negative. The sensitivity test showed that as low as 5 pg of aMPV's DNA could be detected by DIG-labeled probe. The samples of 605 broilers and 122 ducks were collected from flocks in Shandong province,then dot blot hybridization was used to detect avian metapneumovirus,The results show that the detection rates of the two kinds of brids are 36.59% and 34.51% ,respectively. The results showed that the probe could be used in the detection of avian metapneumovirus. This investigation also shows that aMPV was presence in China and the infection is common in broilers and ducks on some farms in Shandong province.

  7. West Virginia Community Colleges.

    Science.gov (United States)

    Miller, Eldon L.; Dziagwa, Constance E.

    1997-01-01

    Discusses efforts over the past 25 years to formalize the role of West Virginia's community colleges in the context of the state's rural character and low college graduation rates. Describes a reorganization following a 1987 study by the Carnegie Foundation and state legislation designed to fine tune the colleges' mission. (10 citations) (AJL)

  8. West Greenlandic Eskimo

    DEFF Research Database (Denmark)

    Trondhjem, Naja Blytmann; Fortescue, Michael David

    the principal economic activity. Research projects and language initiatives currently in progress within Greenland will be touched upon, as will the possibilities of communication with North American Inuit. West Greenlandic is unique among the native languages of the North American Arctic and Sub...

  9. The great West Road

    CERN Multimedia

    1975-01-01

    From right to centre the 'Nationale 84' relying Meyrin to Saint-Genis. The fence limits Lab I on that side. From bottom the road leading to the double inclined tunnel linking Lab I and Lab II. On the foreground the ISR building (left) and the West Hall (centre).

  10. Invigorating West China

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    The once-poor west China is growing at a faster rate than the east. The trend is set to continue over the next few years. This is good news for China as the country gears up to shrink the economic divide between eastern and western regions.

  11. JPRS Report, West Europe.

    Science.gov (United States)

    1988-02-18

    member states are to be brought together. The COMETT Program has elicited great interest within the EC. According to Volker Gehmlich, who is active...of a brand name, an efficient distribution mechanism and a com- petitive cost price. The European "winners" are espe- cially West Germany, which has

  12. Database for West Africa

    African Journals Online (AJOL)

    Such database can prove an invaluable source of information for a wide range of agricultural and ... national soil classification systems around the world ... West African Journal of Appl ied Ecology, vol. .... SDB FAO-ISRIC English, French, Spanish Morphology and analytical ..... Furthermore, it will enhance the state of soil.

  13. FAQ: General Questions about West Nile Virus

    Science.gov (United States)

    ... Public Service Videos General Questions About West Nile Virus Recommend on Facebook Tweet Share Compartir On this ... West Nile virus cases? What is West Nile virus? West Nile virus is an arthropod-borne virus ( ...

  14. West Candor Chasma

    Science.gov (United States)

    1996-01-01

    During its examination of Mars, the Viking 1 spacecraft returned images of Valles Marineris, a huge canyon system 5,000 km long, up to 240 km wide, and 6.5 km deep, whose connected chasma or valleys may have formed from a combination of erosional collapse and structural activity. The view shows west Candor Chasma, one of the connected valleys of Valles Marineris; north toward top of frame. The image is a composite of Viking high-resolution (about 80 m/pixel or picture element) images in black and white and low resolution (about 250 m/pixel) images in color. The Viking 1 craft landed on Mars in July of 1976. West Candor Chasma occupies the westernmost part of the large west-northwest-trending trough of Candor Chasma. This section is about 150 km wide. West Candor Chasma is bordered on the north and south by straight-walled cliffs, most likely faults, and on its west by two segments of north-northeast-trending cliffs. The north wall is dissected by landslide scars forming reentrants filled with landslide debris. The south wall shows spur-and-gully morphology and smooth sections. The high-standing central mesa, informally dubbed Red Mesa has several curvilinear reentrants carved into the caprock, whose anomalously colored layers were interpreted to be caused by young hydrothermal alteration products (Geissler et al., 1993, Icarus, v. 106, p. 380-391). Light-colored lobes flow away from the top of the interior stack and then flow around and embay the same layered stack from which they originated. One of these apparent flow features is composed of at least two or perhaps even three huge, superposed, vaguely layered, very rugged, light-colored lobes as much as 100 km long, 20 km wide, and over 2 km thick. The layered deposits below the caprock also merge with a chaotic material that has local lobate fronts and overlaps landslide deposits. Hummocky material, similar in hue to wall rock, fills the southwestern-most region of west Candor Chasma and is perhaps as much as 3

  15. JPRS Report, West Europe.

    Science.gov (United States)

    2007-11-02

    was no tabula rasa politically. He had worked with EEC Commissioners Sicco Mansholt and Henri Simonet, had represented the Young Socialists in the...OF GERMANY Civil-Military Facilitator Role Proposed for Territorial Army (Hans-Lothar Stegmann; TRUPPENPRAXIS, Mar 87) 80 FRANCE GIAT Problems...supportable. The attitude that Mikhail Gorbachev represents the same type of evil helps them to formulate a specific West German role in the Western world

  16. The West Heslerton Assessment

    Directory of Open Access Journals (Sweden)

    Dominic Powlesland

    1999-03-01

    Full Text Available The excavation of the Early Anglo-Saxon or Anglian Settlement at West Heslerton, North Yorkshire, between 1986 and 1995, represents one of the largest excavations conducted in Britain in the last two decades. The project, funded by English Heritage, combined the fundamental needs of rescue and research archaeology. The excavation has produced a wealth of new evidence which is forcing us to re-evaluate much that has been said about the formative period of the English nation.

  17. Drought in West Africa

    Science.gov (United States)

    2007-01-01

    Drought settled over West Africa's Ivory Coast region when wet season rains came late in 2007. Instead of beginning in February, the rainy season didn't start until March, and steady rains didn't start until late March, said the Famine Early Warning System Network. Though the rain had started to alleviate the drought, vegetation was still depressed in parts of Cote d'Ivoire (Ivory Coast) between March 22 and April 6, 2007, when the Moderate Resolution Imaging Spectroradiometer (MODIS) on NASA's Terra satellite captured the data used to make this image. The image shows current vegetation conditions compared to average conditions recorded since 2000. Areas where plants are growing more slowly or more sparsely than average are brown, while areas where vegetation is denser than average are green. The brown tint that dominates the image indicates that plants through most of the country are more sparse than normal. Among the crops affected by the lack of rain was West Africa's cocoa crop. About 70 percent of the world's cocoa comes from West Africa, and Cote d'Ivoire is a top grower, said Reuters. Cocoa prices climbed as the crop fell short. Farmers called the drought the worst in living memory, Reuters said. The delay in rainfall also led to water shortages in parts of Cote d'Ivoire, according to the United Nations Office for the Coordination of Humanitarian Affairs.

  18. West and Central Africa.

    Science.gov (United States)

    Lydie, N; Robinson, N J

    1998-01-01

    This article reviews scientific and other literature during the 1990s that links migration and mobility with the spread of sexually transmitted diseases (STDs), including HIV/AIDS. The focus is on key population groups linked to the spread of HIV and STDs in West and Central Africa: migrant laborers, truck drivers, itinerant traders, commercial sex workers (CSWs), and refugees. Countries with high emigration and immigration tend to have high levels of HIV infection, with the exception of Senegal. The main destination of immigrants are Senegal, Nigeria, and Cote d'Ivoire in West Africa and Cameroon, Congo, Gabon, and Congo in Central Africa. The risk of infection and the spread of HIV is variable among migrants. There is little in the literature that substantiates hypotheses about the strong association between migration and HIV-positive status. Information is needed on the duration, frequency of return visits, living conditions, sexual activities with multiple partners, and information before departure, along the routes, at final destination, and at the time of returns. Action-based research in five West African countries (Burkina Faso, Cote d'Ivoire, Mali, Niger, and Senegal) should produce results in late 1998. Comparable studies in Central Africa are unknown. Regional studies should be complemented by local studies. Prevention would benefit from studies on the relative size of these five population groups by geographic location.

  19. Drop of egg production in chickens by experimental infection with an avian metapneumovirus strain PLE8T1 derived from swollen head syndrome and the application to evaluate vaccine.

    Science.gov (United States)

    Sugiyama, Miki; Koimaru, Hiroyuki; Shiba, Masahiro; Ono, Eriko; Nagata, Tadashi; Ito, Toshihiro

    2006-08-01

    Decreases in egg production and increased incidence of abnormal eggs due to malformation of egg shells were observed in specific pathogen free (SPF) 173-day-old laying hens inoculated intravenously with an avian metapneumovirus (aMPV) strain PLE8T1. This strain was derived from an isolate from broiler birds exhibiting swollen head syndrome (SHS). Some SPF birds inoculated with the virus showed, slight diarrhea without any respiratory symptoms. Thus, the PLE8T1 strain was used as a challenge virus to evaluate efficacy of aMPV vaccines. SPF chickens which received a live attenuated aMPV vaccine (NEMOVAC; Merial) at 7 or 77 days old and an inactivated aMPV vaccine (OVO-4; Merial) at 105 days old were protected against poor egg production caused by the challenge with the PLE8T1 strain. Thus, aMPV, the PLE8T1 strain passaged 22 times after isolation, from birds exhibiting SHS, could induce a drop in egg production in laying hens accompanied by malformation of egg shells. It was suggested that this challenge system could be applied to evaluate the efficacy of aMPV vaccine.

  20. Efficient Reverse Genetics Reveals Genetic Determinants of Budding and Fusogenic Differences between Nipah and Hendra Viruses and Enables Real-Time Monitoring of Viral Spread in Small Animal Models of Henipavirus Infection

    Science.gov (United States)

    Yun, Tatyana; Park, Arnold; Hill, Terence E.; Pernet, Olivier; Beaty, Shannon M.; Juelich, Terry L.; Smith, Jennifer K.; Zhang, Lihong; Wang, Yao E.; Vigant, Frederic; Gao, Junling; Wu, Ping

    2014-01-01

    ABSTRACT Nipah virus (NiV) and Hendra virus (HeV) are closely related henipaviruses of the Paramyxovirinae. Spillover from their fruit bat reservoirs can cause severe disease in humans and livestock. Despite their high sequence similarity, NiV and HeV exhibit apparent differences in receptor and tissue tropism, envelope-mediated fusogenicity, replicative fitness, and other pathophysiologic manifestations. To investigate the molecular basis for these differences, we first established a highly efficient reverse genetics system that increased rescue titers by ≥3 log units, which offset the difficulty of generating multiple recombinants under constraining biosafety level 4 (BSL-4) conditions. We then replaced, singly and in combination, the matrix (M), fusion (F), and attachment glycoprotein (G) genes in mCherry-expressing recombinant NiV (rNiV) with their HeV counterparts. These chimeric but isogenic rNiVs replicated well in primary human endothelial and neuronal cells, indicating efficient heterotypic complementation. The determinants of budding efficiency, fusogenicity, and replicative fitness were dissociable: HeV-M budded more efficiently than NiV-M, accounting for the higher replicative titers of HeV-M-bearing chimeras at early times, while the enhanced fusogenicity of NiV-G-bearing chimeras did not correlate with increased replicative fitness. Furthermore, to facilitate spatiotemporal studies on henipavirus pathogenesis, we generated a firefly luciferase-expressing NiV and monitored virus replication and spread in infected interferon alpha/beta receptor knockout mice via bioluminescence imaging. While intraperitoneal inoculation resulted in neuroinvasion following systemic spread and replication in the respiratory tract, intranasal inoculation resulted in confined spread to regions corresponding to olfactory bulbs and salivary glands before subsequent neuroinvasion. This optimized henipavirus reverse genetics system will facilitate future investigations into

  1. Estudo comparativo de detecção de metapneumovírus humano pelos métodos de PCR e imunofluorescência direta Comparative study of human metapneumovirus detection by PCR and direct immunofluorescence methods

    Directory of Open Access Journals (Sweden)

    Sheila Negrini Parmezan

    2011-08-01

    Full Text Available INTRODUÇÃO: O metapneumovírus humano (MPVh causa infecções respiratórias em crianças, adultos e idosos imunodeprimidos. O diagnóstico é realizado por imunofluorescência (IF ou biologia molecular. OBJETIVO: Detectar o MPVh em amostras clínicas pelos métodos de reação em cadeia da polimerase (PCR e imunofluorescência direta (IFD. RESULTADOS: Das 202 amostras, a positividade foi de 2% e 4% para IFD e RT-PCR, respectivamente. Sensibilidade e especificidade da IFD foram de 50% e 100%, respectivamente, considerando o PCR com transcrição reversa (RT-PCR como padrão-ouro. CONCLUSÃO: O estudo indica a RT-PCR como o melhor método para a identificação de MPVh em amostras clínicas respiratórias e mostra a importância da padronização do teste para inclusão na rotina laboratorial.INTRODUCTION: Human metapneumovirus (HMPV is responsible for respiratory infections in children and immunocompromised adults and elders. It is commonly diagnosed by immunofluorescence or molecular biology. OBJECTIVE: To detect HMPV in clinical samples by polymerase chain reaction (PCR and direct imunofluorescence (DIF methods. RESULTS: Two percent of 202 samples were positive for DIF and 4% of them for reverse transcriptase PCR (RT-PCR, respectively. Considering RT-PCR as gold standard, DIF sensitivity and specificity were 50% and 100%, respectively. CONCLUSION: Not only does the study show that RT-PCR is the best method for HMPV detection in clinical respiratory samples but it also substantiates the importance of test standardization in laboratory routine.

  2. Nucleotide and Predicted Amino Acid Sequence-Based Analysis of the Avian Metapneumovirus Type C Cell Attachment Glycoprotein Gene: Phylogenetic Analysis and Molecular Epidemiology of U.S. Pneumoviruses

    Science.gov (United States)

    Alvarez, Rene; Lwamba, Humphrey M.; Kapczynski, Darrell R.; Njenga, M. Kariuki; Seal, Bruce S.

    2003-01-01

    A serologically distinct avian metapneumovirus (aMPV) was isolated in the United States after an outbreak of turkey rhinotracheitis (TRT) in February 1997. The newly recognized U.S. virus was subsequently demonstrated to be genetically distinct from European subtypes and was designated aMPV serotype C (aMPV/C). We have determined the nucleotide sequence of the gene encoding the cell attachment glycoprotein (G) of aMPV/C (Colorado strain and three Minnesota isolates) and predicted amino acid sequence by sequencing cloned cDNAs synthesized from intracellular RNA of aMPV/C-infected cells. The nucleotide sequence comprised 1,321 nucleotides with only one predicted open reading frame encoding a protein of 435 amino acids, with a predicted Mr of 48,840. The structural characteristics of the predicted G protein of aMPV/C were similar to those of the human respiratory syncytial virus (hRSV) attachment G protein, including two mucin-like regions (heparin-binding domains) flanking both sides of a CX3C chemokine motif present in a conserved hydrophobic pocket. Comparison of the deduced G-protein amino acid sequence of aMPV/C with those of aMPV serotypes A, B, and D, as well as hRSV revealed overall predicted amino acid sequence identities ranging from 4 to 16.5%, suggesting a distant relationship. However, G-protein sequence identities ranged from 72 to 97% when aMPV/C was compared to other members within the aMPV/C subtype or 21% for the recently identified human MPV (hMPV) G protein. Ratios of nonsynonymous to synonymous nucleotide changes were greater than one in the G gene when comparing the more recent Minnesota isolates to the original Colorado isolate. Epidemiologically, this indicates positive selection among U.S. isolates since the first outbreak of TRT in the United States. PMID:12682171

  3. FAQ: West Nile Virus and Dead Birds

    Science.gov (United States)

    ... Education Public Service Videos West Nile Virus & Dead Birds Recommend on Facebook Tweet Share Compartir On This ... dead bird sightings to local authorities. How do birds get infected with West Nile virus? West Nile ...

  4. Trade networks in West Africa

    DEFF Research Database (Denmark)

    Walther, Olivier

    2014-01-01

    To date, most of the literature on trade networks in West Africa has considered networks in a metaphorical way. The aim of this paper is to go one step further by showing how social network analysis may be applied to the study of regional trade in West Africa. After a brief review of the literature......, this exploratory paper investigates two main issues related to regional trade. We start by discussing how recent developments in regional trade in West Africa have contributed to challenging the social structure of traders. We then discuss the changes that have affected the spatiality of regional trade by looking...

  5. [West Nile virus infection].

    Science.gov (United States)

    Pérez Ruiz, Mercedes; Gámez, Sara Sanbonmatsu; Clavero, Miguel Angel Jiménez

    2011-12-01

    West Nile virus (WNV) is an arbovirus usually transmitted by mosquitoes. The main reservoirs are birds, although the virus may infect several vertebrate species, such as horses and humans. Up to 80% of human infections are asymptomatic. The most frequent clinical presentation is febrile illness, and neuroinvasive disease can occur in less than 1% of cases. Spain is considered a high-risk area for the emergence of WNV due to its climate and the passage of migratory birds from Africa (where the virus is endemic). These birds nest surrounding wetlands where populations of possible vectors for the virus are abundant. Diagnosis of human neurological infections can be made by detection of IgM in serum and/or cerebrospinal fluid samples, demonstration of a four-fold increase in IgG antibodies between acute-phase and convalescent-phase serum samples, or by detection of viral genome by reverse transcription-polymerase chain reaction (especially useful in transplant recipients). Since WNV is a biosafety level 3 agent, techniques that involve cell culture are restricted to laboratories with this level of biosafety, such as reference laboratories. The National Program for the Surveillance of WNV Encephalitis allows the detection of virus circulation among birds and vectors in areas especially favorable for the virus, such as wetlands, and provides information for evaluation of the risk of disease in horses and humans.

  6. Discourse Between East and West

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    The first Chinese-hosted cultural dialogue between the East and the West was held in Nishan, Shandong Province The first Nishan Forum on World Civilizations kicked off on September 26 at Nishan Mountain, 25 km southeast

  7. Dr. West and Mr. Summers

    National Research Council Canada - National Science Library

    Roger Kimball

    2002-01-01

      In all the mainstream press coverage of this affair, it was put about that Comet West is a serious scholar, that AfroAmerican Studies is a serious academic discipline, and that Harvard's department...

  8. Why the West?

    Directory of Open Access Journals (Sweden)

    Marc Ferguson

    2009-03-01

    Full Text Available La cuestión de cómo "Occidente" llegó a dominar el mundo durante la era moderna se ha debatido recientemente entre los historiadores. El debate se ha polarizado entre quienes ven en la "modernidad" como resultado de un 'milagro', el proceso cultural único generado en el seno del mismo Occidente, y aquellos que cuestionan este "milagro" como paradigma eurocéntrico, y buscan otros factores para entender y explicar el dominio occidental del mundo económico y político. La literatura tradicional, representada por David Landes en su reciente “La riqueza y la pobreza de las naciones”, atribuye el éxito europeo a sus valores culturales únicos, a sus instituciones sociales y sus prácticas políticas. Este éxito fue completamente "impulsado desde dentro” por estas características. Recientemente, varios historiadores han cuestionado este "paradigma del milagro" como eurocéntrica, y miran a otros factores para comprender y explicar el dominio occidental del mundo económico y político. Después de examinar los recientes trabajos de los historiadores frente a este problema, este artículo trata de colocar la expansión europea en un contexto global, y la comprensión de la Revolución Industrial como una transformación global. Esta perspectiva nos permite entender los cambios tecnológicos y económicos Europeos en el contexto más amplio de patrones de interacción económica y cultural de todo el mundo._____________ABSTRACT:The question of how 'the West' came to dominate the globe during the modern era has been debated recently among historians. The debate has been polarized between those who view 'modernity' as the result of a 'European miracle', the culturally unique and internally generated project of the West, and those who question this 'European miracle' paradigm as Eurocentric, and look to other factors to understand and explain Western economic and political world dominance. The traditional narrative, represented by David

  9. Landscape evolution of West Kunlun

    Science.gov (United States)

    Wang, Bin; Chang, Hong

    2010-05-01

    Constituting the northwestern margin of the Tibetan Plateau, the present-day Western Kunlun is a spectacular mountain range, extending from the Pamir Plateau in the west to the Altyn Tagh fault and the Eastern Kunlun Range in the east. Dramatic climate variations have a significant control on the fluvial dissection, glacial erosion and wind transport. The West Kunlun Mountains is one of the key regions for understanding the uplift history of the Plateau and the climate change of Central Asia. The activities of tectonic and erosion make the topography of West Kunlun Mountains complex and varied. However, due to its remote location, there is still limited knowledge about the geomorphologic and geological features in West Kunlun Mountains .This paper tries to figure out the geomorphologic and geological features of this region qualitatively through studying the data of Shuttle Reader Topography Mission (SRTM) and topographic maps by digital elevation models (DEMs) and constructing profiles, aiming to make clear what functions the tectonics and climate exert on the topography of the West Kunlun Mountains. The West Kunlun Mountains, the northwestern margin of the Qinghai-Tibet Plateau, have strong tectonic activities. From south to north,West Kunlun Mountains developed three major faults: Kangxiwar fracture, North West Kunlun fault and Iron Creek fault. At about 25 Ma B.P., the West Kunlun began to uplift, and from about 5 Ma B.P., the West Kunlun began to grow rapidly. By using topographic analyzing software to make more than 10 vertical profiles across the main faults from south to north, it is obvious that there is a height difference between the upper and lower plate fracture, and the elevation of terrain profiles generally decreases from south to north. There is ~4,500m elevation difference between the main ridge of the West Kunlun Mountains out of Kangxiwar (5500-6000m) and the northern foot of the Tarim Basin (1200-1300m). The same progressively decreasing

  10. Politeness: West and East

    Directory of Open Access Journals (Sweden)

    - Джеффри Лич

    2014-12-01

    Full Text Available The present paper was planned for this issue of our journal, which Geoffrey Leech and I intended to devote to Politeness phenomena across cultures. It is based on his article titled “Politeness: Is there an East-West Divide?” (2005 which he suggested as a theoretical framework and includes results of our discussions held during our personal meetings and our epistolary exchange. Unfortunately the final version of the paper was never read by Geoffrey Leech for the reasons we all sadly know. Nevertheless I decided to publish it as a tribute to him in the knowledge that the result was not going to have the degree of excellence it would have had if he were still with us today. I therefore apologise for any mistakes or misinterpretations of his thoughts that might be found in the paper. The aim of this article is to sum up the main ideas of Politeness Theory presented earlier in Leech 1983, 2003, 2005, and other publications and discuss how that theory applies (or fails to apply to other languages, with the main emphasis on the Russian language and culture. The term ‘maxim’ used in Principles of Pragmatics (Leech 1983 is avoided here as much as possible, as it implies some kind of moral imperative, rather than a pragmatic constraint. Instead, a single constraint, which comprehends all the maxims (the Maxims of Tact, Generosity, Approbation, Modesty, Agreement, Sympathy, and is called the Grand Strategy of Politeness (GSP, is used. The GSP says: In order to be polite, S expresses or implies meanings which place a high value on what pertains to O- his/her wants, qualities, obligation, opinion, feelings (O = other person[s], [mainly the addressee, i.e. H = hearer] or place a low value on what pertains to S (S = self, speaker. The essential point is that these are not separate, independent constraints or maxims: they are instances of the operation of the GSP as ‘super-maxim’ which is an overarching framework for studying linguistic politeness

  11. Anurans Collected in West Malaysia

    DEFF Research Database (Denmark)

    Cedhagen, Tomas

    1997-01-01

    Distributional records and natural history noles are given for anurans collected in West Malaysia 1976. Rano baramica was observed when it was caught by an Ahaetulfa nasula (Serpentes: Colubridae). Rhacophorus leucomystax, Limnonectes limnociulris and Microhyla heymonsi were all found al night on...

  12. Upgrading of the West Area

    CERN Multimedia

    1983-01-01

    The rejigged main hall (EHW1) in the West Area: on background, below the crane, is the brown yoke of the Omega magnet which had been resited. The upgrading was completed by the time in July when 400 GeV protons arrived. See Annual Report 1983 p. 107.

  13. The West in Early Cinema

    NARCIS (Netherlands)

    Verhoeff, Nanna

    2006-01-01

    Verhoeff investigates the emergence of the western genre, made in the first two decades of cinema (1895-1915). By analyzing many unknown and forgotten films from international archives she traces the relationships between films about the American West, their surrounding films, and other popular medi

  14. Metamorphic Rocks in West Irian

    NARCIS (Netherlands)

    Wegen, van der G.

    1971-01-01

    Low-grade metamorphics of West Irian occur to the east of Geelvink Bay associated with two narrow belts of basic and ultrabasic igneous rocks which represent ophiolitic suites of an eugeosynclinical development beginning in Early Mesozoic time. In both of these belts there are indications of regiona

  15. Primary Schooling in West Bengal

    Science.gov (United States)

    Sen, Amartya

    2010-01-01

    With his Nobel Prize award money, Amartya Sen set up the Pratichi Trust which carries out research, advocacy and experimental projects in basic education, primary health care, and women's development in West Bengal and Bangladesh. Professor Sen himself took active interest in this work--helping set the agenda, looking at the evidence from…

  16. West Nile Virus Neuroinvasive Disease

    Directory of Open Access Journals (Sweden)

    J Gordon Millichap

    2009-07-01

    Full Text Available Epidemiological features of West Nile Virus (WNV disease among children (<18 years of age reported to the Centers for Disease Control and Prevention from 1999 through 2007 were analyzed and compared with those of adult WNV neuroinvasive disease (WNND, in a study at CDC&P, Fort Collins, CO.

  17. Team West Virginia/Rome Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Korakakis, Dimitris [West Virginia Univ., Morgantown, WV (United States)

    2017-04-10

    Overall, the team, West Virginia University (WVU) and University of Rome Tor Vergata (UTV), has a goal of building an attractive, low-cost, energy-efficient solar-powered home that represents both the West Virginian and Italian cultures.

  18. Suggested revision for west mexican archeological sequences.

    Science.gov (United States)

    Long, S V; Taylor, R E

    1966-12-16

    A review of the radiocarbon dates and published and unpublished archeological data from the West Mexican states of Sinaloa, Nayarit, Jalisco, and Colima has resulted in a revised tentative chronology for West Mexico.

  19. West Indian Prose Fiction in the Sixties

    Science.gov (United States)

    Brathwaite, Edward

    1971-01-01

    A Review and critical discussion of the West Indian prose fiction in the sixties by one of the best-known poets of the Carribean and a member of the faculty of the University of West Indies, Jamaica. (JM)

  20. 小儿呼吸道人偏肺病毒感染25例流行病学和临床特征分析%Clinical analyses on human metapneumovirus infection in acute respiratory tract of 25 children

    Institute of Scientific and Technical Information of China (English)

    刘恩梅; 谢晓虹; 邓兵; 王莉佳; 黄英; 陈坤华; 杨锡强

    2007-01-01

    目的 探讨小儿呼吸道人偏肺病毒(human metapneumovirus,hMPV)的感染阳性率、流行病学及其临床特征.方法 2002年12月至2003年12月在本科固定时间收集新入院的急性呼吸道感染患儿的鼻咽分泌物,一式两份.1份采用免疫荧光快速诊断方法用于分析包括呼吸道合胞病毒(respiratory syncytial virus,RSV)、腺病毒(adenovirus)、流感病毒(influenza virus) A、B副流感病毒(parainfluenza virus)1、2、3 这7种常见呼吸道病毒;另1份采用RT-PCR的方法检测上述患儿鼻咽分泌物中hMPV的L基因和M基因,鼻病毒与冠状病毒的基因.结果 检出 hMPV阳性标本25例,阳性率为9.7%,主要分布在秋冬季;25例hMPV阳性标本中单纯hMPV感染10例,占40%,15例(60%)系hMPV与其他病毒合并感染,其中包括8例hMPV与RSV合并感染,hMPV与鼻病毒、冠状病毒合并感染各2例,3例标本同时检出hMPV、RSV、鼻病毒为阳性;分析hMPV阳性呼吸道感染患儿的临床特征:男:女=16:9,12例(48%)临床诊断为毛细支气管炎,9例(36%)临床诊断为肺炎,4例(16%)为哮喘急性发作患儿,除hMPV和其他病毒合并感染更容易出现发热外,单纯hMPV感染与hMPV与其他病毒混合感染在发病年龄、性别、病程及临床表现上无明显差异.结论 hMPV是小儿急性呼吸道感染的重要病原,可与呼吸道其他常见病毒混合感染,仅从临床表现上很难将hMPV感染与其他呼吸道病毒感染区别.

  1. 76 FR 68314 - Special Local Regulations; Key West World Championship, Atlantic Ocean; Key West, FL

    Science.gov (United States)

    2011-11-04

    ... SECURITY Coast Guard 33 CFR Part 100 RIN 1625-AA08 Special Local Regulations; Key West World Championship, Atlantic Ocean; Key West, FL AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast... Key West, Florida during the Key West World Championship, a series of high-speed boat races. The...

  2. 75 FR 17463 - Key West Bank, Key West, Florida; Notice of Appointment of Receiver

    Science.gov (United States)

    2010-04-06

    ... From the Federal Register Online via the Government Publishing Office ] DEPARTMENT OF THE TREASURY Office of Thrift Supervision Key West Bank, Key West, Florida; Notice of Appointment of Receiver Notice... sole Receiver for Key West Bank, Key West, Florida, (OTS No. 14929) on March 26, 2010. Dated: March...

  3. Population Structure of West Greenland Narwhals

    DEFF Research Database (Denmark)

    Riget, F.; Dietz, R.; Møller, P.;

    The hypothesis that different populations of narwhals in the West Greenland area exist has been tested by different biomarkers (metal and organochlorine concentrations, stable isotopes and DNA). Samples of muscle, liver, kidney, blubber and skin tissues of narwhals from West Greenland have been...... isotopes could not support the population structure with two West Greenland populations suggested by the genetic study....

  4. 33 CFR 117.622 - West Bay

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false West Bay 117.622 Section 117.622 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Massachusetts § 117.622 West Bay The draw of the West Bay Bridge, mile...

  5. West Nile virus and "poliomyelitis".

    Science.gov (United States)

    Sejvar, James J

    2004-07-27

    West Nile virus (WNV) has recently been associated with a syndrome of acute flaccid paralysis. Most cases of WNV-associated weakness have clinical, histopathologic, and electrophysiologic characteristics indistinguishable from those of poliomyelitis caused by infection with poliovirus. There is debate about the nomenclature of this manifestation of WNV infection. An historical perspective of the term "poliomyelitis" suggests that the term "WNV poliomyelitis" seems appropriate, but members of the neurologic and infectious disease communities should engage in discussion regarding the terminology of this syndrome.

  6. 南京地区儿童人偏肺病毒感染的流行病学及临床特征分析%Molecular epideiological and clinical feature of human metapneumovirus in children with acute respiratory tract infection in Nanjing City

    Institute of Scientific and Technical Information of China (English)

    金玉; 张静; 赵德育; 谢志萍; 高寒春; 高小倩; 张亚婷; 段招军

    2011-01-01

    Objective To investigate the molecular epideiological and clinical feature of human metapneumovirus in children with acute respiratory tract infection in Nanjing city, China. Method Nasopharyngeal aspirates and nasopharyngeal swab were taken from 642 outpatients or hospitalized pediatric patients with acute at the Children Hospital of Nanjing,Jiangsu Province, China, between August 2009 and July 2010. Respiratory speciments were tested for the M gene of hMPV by reverse-transcription polymerase chain reaction (RT-PCR). All RT-PCR positive products were sequenced and phlogenetic analysis was conducted. Result hMPV was detected in 35 (5.5%) of the 642 children. Phylogenetic analysis revealed that 51.4% of the hMPV were B1, 31.4% were A2b. The peak of the positive rate was in April. The majority of the hMPV-positive patients(71.4% ) were 0-1 years old. Of the 35 hMPV-positive patients, 15 (42. 8% ) were co-infected with other respiratory viruses, and human rhinovirus (HRV) were the most common additional respiratory virus. The most common clinical diagnosis was pneumonia (48. 6% ).Conclusion Human metapneumovirus is an important pathogen of acute respiratory tract infection in children in Nanjing city. The subtype B1 was the predominating lineage in 2009-2010 in Nanjing city. No significant differences were found for clinical characteristics between genotype A and genotype B human metapneumovirus infection in children in Nanjing.%目的 了解南京地区儿童人偏肺病毒(hMPV)感染的流行病学特点及临床特征.方法 收集2009年8月至2010年7月南京医科大学附属南京儿童医院住院及门诊呼吸道感染患儿的鼻咽抽吸物(NPA)及咽拭子(NPS)共642例,采用逆转录聚合酶链反应法(RT-PCR)检测hMPV M基因,将阳性PCR扩增产物进行测序、同源性和进化分析.结果 642例标本中共检出hMPV阳性扩增产物35份,检出率为5.5%.系统进化分析显示南京地区hMPV B1型占51.4%,A2b型占31.4%.h

  7. West Nile Virus and wildlife

    Science.gov (United States)

    Marra, P.P.; Griffing, S.; Caffrey, C.; Kilpatrick, A.M.; McLean, R.; Brand, C.; Saito, E.; Dupuis, A.P.; Kramer, Laura; Novak, R.

    2004-01-01

    West Nile virus (WNV) has spread rapidly across North America, resulting in human deaths and in the deaths of untold numbers of birds, mammals, and reptiles. The virus has reached Central America and the Caribbean and may spread to Hawaii and South America. Although tens of thousands of birds have died, and studies of some bird species show local declines, few regionwide declines can be attributed to WNV. Predicting future impacts of WNV on wildlife, and pinpointing what drives epidemics, will require substantial additional research into host susceptibility, reservoir competency, and linkages between climate, mosquitoes, and disease. Such work will entail a collaborative effort between scientists in governmental research groups, in surveillance and control programs, and in nongovernmental organizations. West Nile virus was not the first, and it will not be the last, exotic disease to be introduced to the New World. Its spread in North America highlights the need to strengthen animal monitoring programs and to integrate them with research on disease ecology.

  8. Surface winds over West Antarctica

    Science.gov (United States)

    Bromwich, David

    1993-01-01

    Five winter months (April-August 1988) of thermal infrared satellite images were examined to investigate the occurrence of dark (warm) signatures across the Ross Ice Shelf in the Antarctic continent. These features are inferred to be generated by katabatic winds that descend from southern Marie Byrd Land and then blow horizontally across the ice shelf. Significant mass is added to this airstream by katabatic winds blowing from the major glaciers that flow through the Transantarctic Mountains from East Antarctica. These negatively buoyant katabatic winds can reach the northwestern edge of the shelf - a horizontal propagation distance of up to 1,000 km - 14 percent of the time. Where the airstream crosses from the ice shelf to the ice-covered Ross Sea, a prominent coastal polynya is formed. Because the downslope buoyancy force is near zero over the Ross Ice Shelf, the northwestward propagation of the katabatic air mass requires pressure gradient support. The study shows that the extended horizontal propagation of this atmospheric density current occurred in conjunction with the passage of synoptic cyclones over the southern Amundsen Sea. These cyclones can strengthen the pressure gradient in the interior of West Antarctica and make the pressure field favorable for northwestward movement of the katabatic winds from West Antarctica across the ice shelf in a geostrophic direction. The glacier winds from East Antarctica are further accelerated by the synoptic pressure gradient, usually undergo abrupt adjustment beyond the exit to the glacier valley, and merge into the mountain-parallel katabatic air mass.

  9. 78 FR 79061 - Noise Exposure Map Notice; Key West International Airport, Key West, FL

    Science.gov (United States)

    2013-12-27

    ... Federal Aviation Administration Noise Exposure Map Notice; Key West International Airport, Key West, FL... Administration (FAA) announces its determination that the Noise Exposure Maps submitted by Monroe County for the Key West International Airport under the provisions of the Aviation Safety and Noise Abatement Act...

  10. 78 FR 2916 - Special Local Regulation; West Palm Beach Triathlon Championship, Intracoastal Waterway, West...

    Science.gov (United States)

    2013-01-15

    ... SECURITY Coast Guard 33 CFR Part 100 RIN 1625-AA08 Special Local Regulation; West Palm Beach Triathlon... Intracoastal Waterway, in West Palm Beach, Florida, during the West Palm Beach Triathlon Championship, on Saturday, June 1, 2013. Approximately 1,500 participants are anticipated to participate in the...

  11. PETROCHINA WEST EAST GAS PIPELINE & SALES COMPANY

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ PetroChina West East Gas Pipeline & Sales Company, a regional company directly under PetroChina Company Limited (PetroChina), is responsible for the construction and operation of the West-East Gas Pipeline Project, and the gas marketing and sales of the natural gas market in China.

  12. Briefing : West Africa and its oil

    NARCIS (Netherlands)

    Ellis, S.D.K.

    2003-01-01

    The US war on terrorism and preparations for war against Iraq have enormously increased the strategic value of West African oil reserves. This comes at a time when there have been massive new discoveries in offshore waters. This article focuses on the increased US interests in West African oil. It e

  13. Environmental History in the American West.

    Science.gov (United States)

    Rothman, Hal K.

    1994-01-01

    Asserts that environmental history is an important component of the history of the American west. Discusses the historiography of the American west in relationship to environmental issues. Concludes that environmental history opened the door for scholars to study the way human beings interact with the physical world around them. (CFR)

  14. Permafrost degradation in West Greenland

    DEFF Research Database (Denmark)

    Foged, Niels Nielsen; Ingeman-Nielsen, Thomas

    2012-01-01

    Important aspects of civil engineering in West Greenland relate to the presence of permafrost and mapping of the annual and future changes in the active layer due to the ongoing climatically changes in the Arctic. The Arctic Technology Centre (ARTEK) has worked more than 10 years on this topic......, Kangerlussuaq, Sisimiut and Nuuk. They are situated in continuous, discontinuous and sporadic permafrost zones. We will show examples of detoriation of permafrost related to present local scale climate observations and large scale climate and permafrost simulations modeled numerically with the GIPL model driven...... by HIRHAM climate projections for Greenland up to 2075. The engineering modelling is based on a risk assessment methodology based on a flow diagram which classify the risk of permafrost degradation causing settlement and stability problems for buildings and infrastructures based on relatively simple...

  15. Human Rights in the West

    Directory of Open Access Journals (Sweden)

    Jorgen S. Nielsen

    1996-12-01

    Full Text Available One of the areas of conflict between Islam and the West in today’s world is the concern for human rights. This has sometimes been criticized in the Muslim world as a form of neo-imperialism. It is therefore necessary to understand the various dimensions of human rights, and the various phases through which this concern has grown. In the earliest form, it was an assertion of the rights of the landed aristocracy against those of the monarch. The French revolution, with its emphasis on "liberty, equality and fraternity," for all individuals, provided another dimension. There were many occasions on which individual and organized religion came into conflict during the Middle Ages. The experience of World War II, particularly the atrocities of the Nazis, led to the internationalization of individual rights.

  16. Westing in New Poems and three Persons

    Directory of Open Access Journals (Sweden)

    Ahmet Doğu

    2008-06-01

    Full Text Available Turkısh literature completes a long period effected by İslamic culture and cıvılızatıon in 19th century when it has tendency to west. Like empire, poets and writers also focus on west. They benefits from west in terms of literature and culture. In this way, they realize innovations in the field of literature and culture as beuracracy realizes in military, financial, administrative and maarif fields. This group of people who interest in the private journal sector in cultural field, French literature styles in literature and some sozcial topics evaluate “west issue” in the perspective of current political and social developments. In this context, Tevfik Fikret is in the favour of extention of borders related to westernism. He thinks of adopting west not only in terms of science, art and techniques but also in all social institutions. With the awakening of nation and nationalism, in the perod of National Literature emerging after II. Mesrutiyet besides the rejection of westernism (alafranga, westernism called civilization (muasırlaşma is also emphized. In this article west is evaluated from the point of view of Ziya Gökalp. According to Ziya Gökalp, west is a civilization circle which should be included in. Turkısh people should take their place in this circle by protecting national culture. Mehmet Akif, living in the same period and follwing Islamic style in his works, emphasizes that there should be limitations on the ınnovations taken from west. According to M. Akif, west can only be a model only in terms of science, art and technique. After 1912, M. Akif criticises west more severely

  17. Population Structure of West Greenland Narwhals

    DEFF Research Database (Denmark)

    Riget, F.; Dietz, R.; Møller, P.

    The hypothesis that different populations of narwhals in the West Greenland area exist has been tested by different biomarkers (metal and organochlorine concentrations, stable isotopes and DNA). Samples of muscle, liver, kidney, blubber and skin tissues of narwhals from West Greenland have been...... analysed for heavy metals, organochlorines, stable isotopes and DNA. The obtained results of metal concentrations and DNA were included in the existing database, whereas no previous data on organochlorines and stable isotopes in West Greenland narwhals existed. The metal and POP concentrations and stable...

  18. West Nile virus and the climate.

    Science.gov (United States)

    Epstein, P R

    2001-06-01

    West Nile virus is transmitted by urban-dwelling mosquitoes to birds and other animals, with occasional "spillover" to humans. While the means by which West Nile virus was introduced into the Americas in 1999 remain unknown, the climatic conditions that amplify diseases that cycle among urban mosquitoes, birds, and humans are warm winters and spring droughts. This information can be useful in generating early warning systems and mobilizing timely and the most environmentally friendly public health interventions. The extreme weather conditions accompanying long-term climate change may also be contributing to the spread of West Nile virus in the United States and Europe.

  19. 人偏肺病毒多表位抗原的小鼠免疫应答%Evaluation of the immune response to human metapneumovirus multi-epitope antigen in an mouse model

    Institute of Scientific and Technical Information of China (English)

    李晓燕; 郭丽茹; 孔梅; 邹明; 苏旭

    2015-01-01

    作用下可产生高滴度的特异性抗体,也可激活CTL。 CpG ODN可引起Th1/Th2平衡免疫应答,CpG ODN和铝镁佐剂合用免疫效果最好,商品化佐剂Quickantibody5W也可产生相似的效果。本研究为今后hMPV表位疫苗开发、诊断方法建立及hMPV流行病学研究等奠定了基础。%Objective To evaluate the immune response triggered by an in-house constructed hu-man metapneumovirus multi-epitope antigen ( MEA) in a mouse model .Methods Female SPF BALB/c mice at age 4-6 weeks were used in the study and divided into 7 groups.Mice in the five groups including MEA+oligodeoxynucleotides containing CpG motifs ( CpG ODN) intraperitoneal injection ( i.p.) treatment group, MEA+Alum i.p.treatment group, MEA+Alum+CpG ODN i.p.treatment group, MEA+CpG ODN intranasal (i.n.) treatment group and MEA+Alum+CpG ODN i.n.treatment group were immunized three times on days 0, 14 and 21, and those in the other experimental group were immunized intramuscularly with MEA+Quickantibody5W on days 0 and 21.A control group without treatment was set up accordingly .All mice were sacrificed two weeks after the last immunization .Antibodies including IgG , IgG1, IgG2a and IgA in serum samples were detected by ELISA .MTS assay was performed to analyze the proliferation of lympho-cytes.The cytotoxicity of cytotoxic T lymphocytes (CTL) was measured by LDH assay.Flow cytometry was used to detect T lymphocyte subsets .The cytokines secreted by T helper cells ( Th1 and Th2) were analyzed with Bio-Rad Liquid Chips.Results High titers of IgG, IgG1 and IgG2a antibodies were produced in MEA treated mice except for those in intranasal treatment groups .Serum samples from three groups including the MEA+Alum i.p., MEA+Alum+CpG ODN i.p.and MEA+Quickantibody5W i.m.treatment groups were positive for IgA antibody .The highest titer of IgA antibody was detected in mice from the MEA+Alum+CpG ODN i.p.treatment group, which was 2.15×103.Compared with the control group

  20. DOLLY SODS WILDERNESS, WEST VIRGINIA.

    Science.gov (United States)

    Englund, Kenneth J.; Hill, James J.

    1984-01-01

    Coal, the principal mineral resource of the Dolly Sods Wilderness, West Virginia is in at least seven beds of low- to medium-volatile bituminous rank. Of these beds, four are of sufficient thickness, quality, and extent to contain demonstrated coal resources which are estimated to total about 15. 5 million short tons in areas of substantiated coal resource potential. A Small-scale development of the coal resources of the Dolly Sods Wilderness has been by several shallow adits which provided fuel for locomotives during early logging operations and by a one truck mine. All mine entries are now abandoned. Peat, shale, clay, and sandstone, occur in the area but because of remoteness of markets and inaccessability they are not classified as resources in this report. Natural gas may occur in rocks underlying the area, but because of a lack of subsurface information an estimate of resource potential has not been made. No evidence of metallic-mineral resources was found during this investigation.

  1. IAU South West Asian ROAD

    Science.gov (United States)

    Mickaelian, Areg; Azatyan, Naira; Farmanyan, Sona; Mikayelyan, Gor

    2016-10-01

    Armenia is hosting the IAU South West Asian (SWA) Regional Office of Astronomy for Development (ROAD). It is a county of ancient astronomy and is also rich in modern astronomical facilities and infrastructures, hence may successfully serve as a regional center for various activities. Byurakan Astrophysical Observatory (BAO) has 2.6m and 1m Schmidt, as well as a number of smaller telescopes that are an observational basis for joint projects and collaborations. Armenian Virtual Observatory (ArVO) is hosting astronomical databases, such as the Digitized First Byurakan Survey (DFBS) and may also serve as a basis for development of VO structures in this region. Recently we have conducted a number of new activities; a meeting on ``Relation of Astronomy to other Sciences, Culture and Society" (RASCS) was organized by BAO and Armenian Astronomical Society (ArAS) in Oct 2014 in Byurakan. Activities related to Archaeoastronomy and Astronomy in Culture (AAC) were initiated as well. Discussions on future Armenian-Iranian collaboration in astronomy were carried out, including an Armenian-Iranian Astronomical Workshop held in Oct 2015 in Byurakan. Similar workshops have been carried out between BAO and Abastumani Astronomical Observatory (AbAO, Georgia) since 1974.

  2. Does the geoid drift west?

    Science.gov (United States)

    Backus, G. E.; Parker, R. L.; Zumberge, M. A.

    1985-01-01

    In 1970 Hide and Malin noted a correlation of about 0.8 between the geoid and the geomagnetic potential at the Earth's surface when the latter is rotated eastward in longitude by about 160 degrees and the spherical harmonic expansions of both functions are truncated at degree 4. From a century of magnetic observatory data, Hide and Malin inferred an average magnetic westward drift rate of about 0.27 degrees/year. They attributed the magnetic-gravitational correlation to a core event at about 1350 A.D. which impressed the mantle's gravity pattern at long wavelengths onto the core motion and the resulting magnetic field. The impressed pattern was then carried westward 160 degrees by the nsuing magnetic westward drift. An alternative possibility is some sort of steady physical coupling between the magnetic and gravitational fields (perhaps migration of Hide's bumps on the core-mantle interface). This model predicts that the geoid will drift west at the magnetic rate. On a rigid earth, the resulting changes in sea level would be easily observed, but they could be masked by adjustment of the mantle if it has a shell with viscosity considerably less than 10 to the 21 poise. However, steady westward drift of the geoid also predicts secular changes in g, the local acceleration of gravity, at land stations. These changes are now ruled out by recent independent high-accuracy absolute measurements of g made by several workers at various locations in the Northern Hemisphere.

  3. West Bank Gaza Geo-MIS System

    Data.gov (United States)

    US Agency for International Development — The Geo-MIS System is USAID/West Bank and Gaza's primary system for capturing and managing projectrelated information. Its purpose is to assist USAID and its...

  4. WEST AND EAST REGIONS OF GHANA

    African Journals Online (AJOL)

    mobilisation and saving as well as records keeping in PMCS in the Upper West and. East Regions. 1. ..... money to meet the maintenance of pump facilities including pump site .... are given some incentives as motivation, the data show that the.

  5. West Nile Virus Mimicking Herpes Encephalitis

    Directory of Open Access Journals (Sweden)

    J Gordon Millichap

    2006-09-01

    Full Text Available A 3-year-old male child with suspected herpes simplex virus encephalitis who asubsequently tested positive for West Nile virus is reported from Schneider Children’s Medical Center, Petah Tikva, Israel.

  6. NNDSS - Table II. West Nile virus disease

    Data.gov (United States)

    U.S. Department of Health & Human Services — NNDSS - Table II. West Nile virus disease - 2017. In this Table, provisional cases of selected notifiable diseases (≥1,000 cases reported during the preceding...

  7. West Coast Rockfish Conservation Areas, 2015

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — These data delineate Rockfish Conservation Areas (RCA) off the West Coast of the United States for 2015. There are three types of areas closures depicted in this...

  8. West Coast Observing System (WCOS) Temperature Data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The West Coast Observing System (WCOS) project provides access to temperature and currents data collected at four of the five National Marine Sanctuary sites,...

  9. Libraries in West Virginia: MedlinePlus

    Science.gov (United States)

    ... this page: https://medlineplus.gov/libraries/westvirginia.html Libraries in West Virginia To use the sharing features ... enable JavaScript. Bridgeport United Hospital Center Health Sciences Library 327 Medical Park Dr Bridgeport, WV 26330 681- ...

  10. Legume Diversity Patterns in West Central Africa

    NARCIS (Netherlands)

    Estrella, de la M.; Mateo, M.A.; Wieringa, J.J.; Mackinder, B.; Munoz, J.

    2012-01-01

    Objectives - Species Distribution Models (SDMs) are used to produce predictions of potential Leguminosae diversity in West Central Africa. Those predictions are evaluated subsequently using expert opinion. The established methodology of combining all SDMs is refined to assess species diversity withi

  11. NNDSS - Table II. West Nile virus disease

    Data.gov (United States)

    U.S. Department of Health & Human Services — NNDSS - Table II. West Nile virus disease - 2015.In this Table, provisional cases of selected notifiable diseases (≥1,000 cases reported during the preceding...

  12. Drought May Beckon Bigger West Nile Outbreaks

    Science.gov (United States)

    ... page: https://medlineplus.gov/news/fullstory_163495.html Drought May Beckon Bigger West Nile Outbreaks Dry environment ... found that epidemics were larger during years of drought. There were also bigger outbreaks in areas that ...

  13. West Nile Virus: Symptoms and Treatment

    Science.gov (United States)

    ... treatment, such as intravenous fluids, pain medication, and nursing care. If you think you or a family member might have West Nile virus disease, talk with your health care provider. To learn more about treatment, visit ...

  14. Eighteenth annual West Coast theoretical chemistry conference

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-05-01

    Abstracts are presented from the eighteenth annual west coast theoretical chemistry conference. Topics include molecular simulations; quasiclassical simulations of reactions; photodissociation reactions; molecular dynamics;interface studies; electronic structure; and semiclassical methods of reactive systems.

  15. West Nile virus and the climate

    OpenAIRE

    Epstein, Paul R.

    2001-01-01

    West Nile virus is transmitted by urban-dwelling mosquitoes to birds and other animals, with occasional “spillover” to humans. While the means by which West Nile virus was introduced into the Americas in 1999 remain unknown, the climatic conditions that amplify diseases that cycle among urban mosquitoes, birds, and humans are warm winters and spring droughts. This information can be useful in generating early warning systems and mobilizing timely and the most environmentally friendly public h...

  16. Potential seaways across West Antarctica

    Science.gov (United States)

    Vaughan, David G.; Barnes, David K. A.; Fretwell, Peter T.; Bingham, Robert G.

    2011-10-01

    The West Antarctic ice sheet (WAIS) has long been considered vulnerable to rapid retreat and today parts are rapidly losing ice. Projection of future change in WAIS is, however, hampered by our poor understanding of past changes, especially during interglacial periods that could be analogs for the future, but which undoubtedly provide an opportunity for testing predictive models. We consider how ice-loss would open seaways across WAIS; these would likely alter Southern Ocean circulation and climate, and would broadly define the de-glacial state, but they may also have left evidence of their existence in the coastal seas they once connected. We show the most likely routes for such seaways, and that a direct seaway between Weddell and Ross seas, which did not pass through the Amundsen Sea sector, is unlikely. Continued ice-loss at present rates would open seaways between Amundsen and Weddell seas (A-W), and Amundsen and Bellingshausen seas (A-B), in around one thousand years. This timescale indicates potential future vulnerability, but also suggests seaways may have opened in recent interglacial periods. We attempt to test this hypothesis using contemporary bryozoan species assemblages around Antarctica, concluding that anomalously high similarity in assemblages in the Weddell and Amundsen seas supports recent migration through A-W. Other authors have suggested opening of seaways last occurred during Marine Isotope Stage 7a (209 ka BP), but we conclude that opening could have occurred in MIS 5e (100 ka BP) when Antarctica was warmer than present and likely contributed to global sea levels higher than today.

  17. On Universal Telecommunication Service and West China Development

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    This paper aims at studying the relationship between universal telecommunication service and West China development. It, firstly, introduces our country's demands for universal telecommunication service; secondly, analyses the demand of West China development for the universal telecommunication service; thirdly, depicts the development status of telecommunication industry of the west region; fourthly, points out the significance of universal telecommunication service to the west area; and lastly, brings forward the connotation of universal telecommunication service of west area.

  18. Evolution of the West Siberian Basin

    Energy Technology Data Exchange (ETDEWEB)

    Vyssotski, A.V. [Chevron, 1500 Louisiana Street, Houston (United States); Vyssotski, V.N. [TNK-BP, 1 Arbat St, Moscow 119019 (Russian Federation); Nezhdanov, A.A. [OOO TyumenNIIgiprogas, 2 Vorovskogo Str., Tyumen 625019 (Russian Federation)

    2006-01-01

    The West Siberian Basin is one of the largest intra-cratonic basins of the world and an important hydrocarbon province of Russia. Perhaps the most important geologic event in Siberia was the emplacement of basalts around {approx} 250Ma (i.e. Permo-Triassic boundary) covering an area of about 5x10{sup 6} km{sup 2}. This volcanism may be responsible for a mass extinction that occurred around Permian-Triassic time. The pre-basaltic rifting event was limited to the north-northeastern sector of the basin. Initial basin wide subsidence took place in the Jurassic as a result of which the western part of Siberia became the West Siberian Basin bounded by uplifts to the east and to the west. One of the surprising aspects of the West Siberian Basin is the abundance of sub-vertical faults believed to be result of strike-slip movement. While intra-plate inversions and fault reactivation structures have been observed in many cratons, sub-vertical faults observed in the West Siberian Basin are unique because of their geometries and abundance. The differentiation between the effects of tectonics and eustasy in cratonic basins is simple-the global eustatic signal is basin-wide with regional and local tectonics playing an overprinting role. Thus, the Middle Jurassic-Turonian 1st, 2nd, and 3rd order cycles in the West Siberian Basin were primarily driven by eustasy. The Middle Jurassic-Turonian series can be subdivided into two second-order and 16 third-order transgressive-regressive cycles (within dataset extent). Fourth-order cycles appear to be controlled by delta shifting. Although extensively studied, a number of fundamental questions regarding the origin and evolution of the West Siberian Basin remain unresolved or poorly documented in the literature. [Author].

  19. 78 FR 22193 - Special Local Regulations; West Palm Beach Triathlon Championship, Intracoastal Waterway; West...

    Science.gov (United States)

    2013-04-15

    ... SECURITY Coast Guard 33 CFR Part 100 RIN 1625-AA08 Special Local Regulations; West Palm Beach Triathlon... Palm Beach, Florida, during the West Palm Beach Triathlon Championship, on Saturday, June 1, 2013. Approximately 1,500 participants are anticipated to participate in the triathlon. The special local...

  20. 77 FR 56125 - Approval and Promulgation of Air Quality Implementation Plans; West Virginia; Amendments to West...

    Science.gov (United States)

    2012-09-12

    ..., EPA published a notice of proposed rulemaking (NPR) for West Virginia. 77 FR 10423. The ] NPR proposed... adopted a PSD program under 45 CSR 14, and the program became part of the West Virginia SIP. See 51 FR... review by the Office of Management and Budget under Executive Order 12866 (58 FR 51735, October 4,...

  1. Grizzly West: A Failed Attempt to Reintroduce Grizzly Bears in the Mountain West

    Directory of Open Access Journals (Sweden)

    Douglas M. Richardson

    2016-05-01

    Full Text Available Reviewed: Grizzly West: A Failed Attempt to Reintroduce Grizzly Bears in the Mountain West. By Michael M. Dax. Lincoln, NE: University of Nebraska Press, 2015. x + 289 pp. US$ 37.50. ISBN 978-0-8032-6673-5.

  2. Blooming Seas West of Ireland

    Science.gov (United States)

    2007-01-01

    For several weeks in May and early June, daily satellite images of the North Atlantic Ocean west of Ireland have captured partial glimpses of luxuriant blooms of microscopic marine plants between patches of clouds. On June 4, 2007, the skies over the ocean cleared, displaying the sea's spring bloom in brilliant color. A bright blue bloom stretches north from the Mouth of the River Shannon and tapers off like a plume of blue smoke north of Clare Island. (In the large image, a second bloom is visible to the north, wrapping around County Donegal, on the island's northwestern tip.) The image was captured by the Moderate Resolution Imaging Spectroradiometer (MODIS) on NASA's Terra satellite. Cold, nutrient-stocked water often wells up to the surface from the deeper ocean along coastal shelves and at the edges of ocean currents. When it does, it delivers a boost of nutrients that fuel large blooms of single-celled plants collectively known as phytoplankton. The plants are the foundation of the marine food web, and their proliferation in this area of the North Atlantic explains why the waters of western Ireland support myriad fisheries and populations of large mammals like seals, whales, and dolphins. Like plants on land, phytoplankton make their food through photosynthesis, harnessing sunlight for energy using chlorophyll and other light-capturing pigments. The pigments change the way light reflects off the surface water, appearing as colorful swirls of turquoise and green against the darker blue of the ocean. Though individually tiny, collectively these plants play a big role in Earth's carbon and climate cycles; worldwide, they remove about as much carbon dioxide from the atmosphere during photosynthesis as land plants do. Satellites are the only way to map the occurrence of phytoplankton blooms across the global oceans on a regular basis. That kind of information is important not only to scientists who model carbon and climate, but also to biologists and fisheries

  3. Political struggle and West Indies cricket

    Directory of Open Access Journals (Sweden)

    Jay R. Mandle

    1996-01-01

    Full Text Available [First paragraph] An Area of Conquest: Popular Democracy and West Indies Cricket Supremacy. HILARY McD BECKLES (ed.. Kingston: Ian Randle, 1995. xviii + 154 pp. (Paper n.p. Liberation Cricket: West Indies Cricket Culture. HILARY McD BECKLES & BRIAN STODDART (eds.. Kingston: Ian Randle, 1995. xii + 403 pp. (Paper n.p. We discovered cricket's importance in the English-speaking Caribbean nearly thirty years ago when we took up our first post in the West Indies. Exploring the Mona campus of the University of the West Indies, we were alarmed to observe so many people who appeared to be hearing-disabled. Wherever we went we found people with ear-pieces who were slightly distracted and at the same time prone to violent mood swings, ranging from the depths of despair to enormous elation. Uncertain about the meaning of what we observed, but reluctant, as newcomers, to reveal our ignorance of public health problems in the region, we delayed inquiring about hearing disabilities until we could confide our concerns to a trusted friend. At first convulsed with laughter, she finally recovered sufficiently to assure us that the people of the West Indies did not suffer disproportionately from hearing loss. Rather, the large numbers of people with ear-pieces were listening to a cricket test match!

  4. Future markers of the West Greenlandic

    DEFF Research Database (Denmark)

    Trondhjem, Naja Blytmann

    2010-01-01

    Abstract West Greenlandic, a polysynthetic language, belongs to Inuit languages. In Inuktitut (Canada) and West Greenlandic (Inuit languages) tense is marked by optional tense suffixes and in both languages the temporal systems are based on a future/ non-future opposition. In Inuktitut the tense......, and it is used when none of the others can be used. It seems that some of the tense suffixes i.e. past (-sima, perfective, perfect, preterite) and future (-ler, begin, be about to, near future) originally had a more or less concrete aspectual meanings and have developed into more abstract tense meanings...... suffixes have developed a complicated remoteness system. In West Greenlandic the future tense suffixes have a distinction between vague and inevitable future, and the past time suffixes have developed different perfect meanings. In Iñupiaq (Alaska), the temporal system is based on an opposition between...

  5. Glucose intolerance in the West African Diaspora

    DEFF Research Database (Denmark)

    Nielsen, Jannie; Christensen, Dirk Lund

    2011-01-01

    In the United States, Black Americans are largely descendants of West African slaves; they have a higher relative proportion of obesity and experience a higher prevalence of diabetes than White Americans. However, obesity rates alone cannot explain the higher prevalence of type 2 diabetes. Type 2...

  6. Epidemiology: Malaria in a warmer West Africa

    Science.gov (United States)

    Caminade, C.; Jones, A. E.

    2016-11-01

    Malaria risk in West Africa is expected to fall (western region) or remain the same (eastern region) in response to climate change over the twenty-first century. This is primarily due to extreme temperature conditions projected under a high greenhouse gas emissions scenario.

  7. Design and manufacturing of WEST Baffle

    Energy Technology Data Exchange (ETDEWEB)

    Batal, Tristan, E-mail: tristan.batal@cea.fr [CEA, IRFM, F-13108 Saint-Paul-Lez-Durance (France); Firdaouss, Mehdi; Richou, Marianne; Ferlay, Fabien; Larroque, Sébastien; Gargiulo, Laurent [CEA, IRFM, F-13108 Saint-Paul-Lez-Durance (France); Dupas, Timothée [SODITECH Ingénierie SA, 1 bis allée des gabians, 06150 Cannes la Bocca (France); Verger, Jean-Marc; Doceul, Louis; Samaille, Franck; Bucalossi, Jérôme [CEA, IRFM, F-13108 Saint-Paul-Lez-Durance (France); Salami, Michael [AVANTIS groupe, 12 route de Saint Mathieu, 06130 Grasse (France)

    2015-10-15

    Highlights: • Disruption's torque in the PFC was simulated thanks to ANSYS. • The ANSYS thermal results comply with WEST project requirements. • The cycling analysis complies with WEST project requirements. • 316L components comply with A level RCC-MRx criteria. - Abstract: The Tore Supra tokamak is being transformed in an X-point divertor fusion device in the frame of the WEST project, launched in support to the Iter tungsten divertor strategy. The WEST Baffle aims to evacuate particles by creating a toroidal pumping throat. It also protects the lower divertor cooling pipes and the passive stabilization plate from heat fluxes. The Baffle is made of actively cooled plasma facing components (PFCs), with underneath a passive stabilization plate and a support beam. The Baffle design is presented in a first part. In a second time the different ANSYS simulations are described: electromagnetic, thermal and mechanical results are presented. The design choices are justified for the different results obtained thanks to the simulation. In a final part, some aspects of the PFC manufacturing are presented.

  8. Large Acrobatic Play Pilgrimage to the West

    Institute of Scientific and Technical Information of China (English)

    马洁

    2006-01-01

    Hosted by Ministry of Culture and Guangzhou Municipal People's Government,Guangzhou Bureau of Culture organized a list of first-class singing and dancing and acrobatic plays in Guangzhou in September 2006. Among these plays, large original acrobatic play Pilgrimage to the West of Guangzhou Acrobatic Troupe went on the stage during October 12th and November 5th.

  9. Wildlife resources of the West African savanna

    NARCIS (Netherlands)

    Bie, de S.

    1991-01-01

    The wild fauna in Africa is a renewable resource and its overexploitation has led to the depletion of animal populations. This thesis focusses on the ecological characterization of the ungulate community of the West African savanna, with special reference to the Biosphere Reserve 'Boucle du

  10. Mastomys natalensis and Lassa fever, West Africa.

    Science.gov (United States)

    Lecompte, Emilie; Fichet-Calvet, Elisabeth; Daffis, Stéphane; Koulémou, Kékoura; Sylla, Oumar; Kourouma, Fodé; Doré, Amadou; Soropogui, Barré; Aniskin, Vladimir; Allali, Bernard; Kouassi Kan, Stéphane; Lalis, Aude; Koivogui, Lamine; Günther, Stephan; Denys, Christiane; ter Meulen, Jan

    2006-12-01

    PCR screening of 1,482 murid rodents from 13 genera caught in 18 different localities of Guinea, West Africa, showed Lassa virus infection only in molecularly typed Mastomys natalensis. Distribution of this rodent and relative abundance compared with M. erythroleucus correlates geographically with Lassa virus seroprevalence in humans.

  11. Vaccines in Development against West Nile Virus

    Directory of Open Access Journals (Sweden)

    Frederic Tangy

    2013-09-01

    Full Text Available West Nile encephalitis emerged in 1999 in the United States, then rapidly spread through the North American continent causing severe disease in human and horses. Since then, outbreaks appeared in Europe, and in 2012, the United States experienced a new severe outbreak reporting a total of 5,387 cases of West Nile virus (WNV disease in humans, including 243 deaths. So far, no human vaccine is available to control new WNV outbreaks and to avoid worldwide spreading. In this review, we discuss the state-of-the-art of West Nile vaccine development and the potential of a novel safe and effective approach based on recombinant live attenuated measles virus (MV vaccine. MV vaccine is a live attenuated negative-stranded RNA virus proven as one of the safest, most stable and effective human vaccines. We previously described a vector derived from the Schwarz MV vaccine strain that stably expresses antigens from emerging arboviruses, such as dengue, West Nile or chikungunya viruses, and is strongly immunogenic in animal models, even in the presence of MV pre-existing immunity. A single administration of a recombinant MV vaccine expressing the secreted form of WNV envelope glycoprotein elicited protective immunity in mice and non-human primates as early as two weeks after immunization, indicating its potential as a human vaccine.

  12. Writing the New West: A Critical Review

    Science.gov (United States)

    Robbins, Paul; Meehan, Katharine; Gosnell, Hannah; Gilbertz, Susan J.

    2009-01-01

    A vast and growing interdisciplinary research effort has focused on the rise of the so-called New West, purportedly the product of regional socioeconomic, political, and ecological upheavals in states like Montana and Colorado. Reviewing the growing research on this problem in sociology, economics, geography, and conservation science, this article…

  13. West Indian Sojourners in Guatemala and Honduras

    Directory of Open Access Journals (Sweden)

    Ronald N. Harpelle

    2012-12-01

    Full Text Available Review of: Race, Nation, and West Indian Immigration to Honduras, 1890-1940. Glenn A. Chambers. Baton Rouge: Louisiana State University Press, 2010. xii +202 pp. (Cloth US$ 35.00Black Labor Migration in Caribbean Guatemala, 1882-1923. Frederick Douglass Opie. Gainesville: University Press of Florida, 2009. 145 pp. (Cloth US$ 65.00

  14. Why We Need West Nile Virus Testing

    Centers for Disease Control (CDC) Podcasts

    2016-09-27

    Dr. Rodrigo Hasbun, a professor of infectious diseases at McGovern Medical School at UT Health, discusses the need for West Nile virus testing in Texas.  Created: 9/27/2016 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 9/27/2016.

  15. Education and Koranic Literacy in West Africa

    OpenAIRE

    Easton, Peter

    1999-01-01

    The note examines the practical, and literate skills that students acquire at different levels in West African Koranic schools. It is a long-standing parallel system of education, yet, relatively unknown to development planers, thus seldom taken into explicit account in their policies, and strategies. Islamic educational systems have been present since the seventh century, and by the tenth ...

  16. West Florida shelf upwelling: Origins and pathways

    Science.gov (United States)

    Weisberg, Robert H.; Zheng, Lianyuan; Liu, Yonggang

    2016-08-01

    Often described as oligotrophic, the west Florida continental shelf supports abundant fisheries, experiences blooms of the harmful alga, Karenia brevis, and exhibits subsurface chlorophyll maxima evident in shipboard and glider surveys. Renewal of inorganic nutrients by the upwelling of deeper ocean water onto the shelf may account for this, but what are the origins and pathways by which such new water may broach the shelf break and advance toward the shoreline? We address these questions via numerical model simulations of pseudo-Lagrangian, isopycnic water parcel trajectories. Focus is on 2010, when the west Florida shelf was subjected to an anomalously protracted period of upwelling caused by Gulf of Mexico Loop Current interactions with the shelf slope. Origins and pathways are determined by integrating trajectories over successive 45 day intervals, beginning from different locations along the shelf break and at various locations and depths along the shelf slope. Waters upwelling across the shelf break are found to originate from relatively shallow depths along the shelf slope. Even for the anomalous 2010 year, much of this upwelling occurs from about 150 m and above, although waters may broach the shelf break from 300 m depth, particularly in the Florida Panhandle. Such interannual renewal of west Florida shelf waters appears to have profound effects on west Florida shelf ecology.

  17. West Virginia State Budgeting for Education.

    Science.gov (United States)

    Meckley, Richard

    County school districts in West Virginia are facing a funding crisis. Significant outmigration resulting in decreased school enrollment has caused the state legislature to change the state school aid formula, forcing school districts to lay off large numbers of low-seniority employees. The state legislature's perception that public schools are…

  18. American Indian Studies in West Germany.

    Science.gov (United States)

    Bartelt, H. Guillermo

    1986-01-01

    Interest in the American Indian in West Germany is high. Romantic notions, derived from the novels of 19th century German writer Karl May and American westerns shown on German television, combined with a subtle anti-Americanism might be responsible for the American Indian Movement (AIM) support groups that have been forming among students and…

  19. West African spatial patterns of economic activities

    DEFF Research Database (Denmark)

    Walther, Olivier; Howard, Allen; Retaillé, Denis

    2015-01-01

    Over the last 30 years, two different bodies of literature developed by both US historians and francophone geographers have moved toward similar conclusions regarding West African economic spatial patterns. Despite their different backgrounds, both the ‘spatial factor’ approach promoted...

  20. West Point student drops out / Jorgen Johansson

    Index Scriptorium Estoniae

    Johansson, Jorgen

    2001-01-01

    Läti kohus mõistis välja kahjutasu Juris Matusevics'ilt, kes rikkus Läti kaitsejõududega sõlmitud kokkulepet, mis kohustas teda pärast USA West Pointi sõjaväeakadeemia lõpetamist teenima viis aastat Läti armees

  1. Ecosystem variability in west Greenland waters

    DEFF Research Database (Denmark)

    Buch, E.; Pedersen, Søren Anker; Ribergaard, M. H.

    2004-01-01

    A review of the climate conditions off West Greenland during the past 50 years shows large variability in the atmospheric, oceanographic and sea-ice variables, as well as in fish stocks. A positive relationship is found between water temperature and the recruitment of cod and redfish, whereas the...

  2. The lithospheric mantle below southern West Greenland

    DEFF Research Database (Denmark)

    Sand, Karina Krarup; Waight, Tod Earle; Pearson, D. Graham

    2009-01-01

    Geothermobarometry of primarily garnet lherzolitic xenoliths from several localities in southern West Greenland is applied to address the diamond potential, pressure and temperature distribution and the stratigraphy of the subcontinental lithospheric mantle ~600 Ma ago. The samples are from kimbe...... into the reworked Archean North of the Naqssugtoqidian deformation front....

  3. West Tennessee ACEI 2006 Fall Conference

    Science.gov (United States)

    Clifford, Anna; Hailey, Beth

    2007-01-01

    This article presents the West Tennessee ACEI 2006 Fall Conference held at Union University in Jackson, Tennessee on October 14, 2006. The conference theme, Turning the Pages: A Focus on Children's Literature, was emphasized throughout the day. During the conference, the early childhood classroom teachers, preservice teachers, and administrators…

  4. South West Province Eye Care Programme, Cameroon

    Directory of Open Access Journals (Sweden)

    Joseph Enyegue Oye

    2005-06-01

    Full Text Available Situation analysis The South West Province of Cameroon is one of two English speaking provinces, much of which is situated in the equatorial rainforest. Most of the estimated 1.2 million inhabitants live in rural areas. The main occupation is agriculture for subsistence and employment in agro-industrial estates.

  5. Transcending Communication Barriers with West Africa.

    Science.gov (United States)

    Ampadu, Lena

    Americans doing business with West Africans are limited in their ability to communicate successfully in that part of the world because of language, stereotyping, and ethnocentrism. Americans must become accustomed to British patterns of speech and writing. Stereotypes of Africa, its people, and its cultures perpetuated by the media keep Americans…

  6. Hangzhou:EAST CITY,WEST LAKE

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    @@ View over West Lake,Hangzhou is in Zhejiang Province,eastern coast area of China.It is one of the most important tourism cities here,famous for its natural beauty and historical and cultural heritages,and the political,economic and cultural center of Zhejiang province as well.

  7. Jimma town, Jimma Zone, South West Ethiopia

    African Journals Online (AJOL)

    and determinants of under five mortality in Jimma ton, Jimma Zone, South West. Ethiopia. All under ... and malnutrition (2). These can vary in .... risk factor. Children breast fed for less than one year were 34 times more likely to die before age of ...

  8. NNDSS - Table II. West Nile virus disease

    Data.gov (United States)

    U.S. Department of Health & Human Services — NNDSS - Table II. West Nile virus disease - 2016. In this Table, provisional* cases of selected†notifiable diseases (≥1,000 cases reported during the preceding...

  9. West Point student drops out / Jorgen Johansson

    Index Scriptorium Estoniae

    Johansson, Jorgen

    2001-01-01

    Läti kohus mõistis välja kahjutasu Juris Matusevics'ilt, kes rikkus Läti kaitsejõududega sõlmitud kokkulepet, mis kohustas teda pärast USA West Pointi sõjaväeakadeemia lõpetamist teenima viis aastat Läti armees

  10. Marine fishery possibilities of the west coast of India

    Digital Repository Service at National Institute of Oceanography (India)

    Panikkar, N.K.

    Marine fishery activity of the west coast of India is discussed. Sea fish production from the west coast of India makes three fourths of total fish production from Indian coasts. Kerala accounts for the largest production of fish in India...

  11. Hydrological modelling of the west coast of India

    Digital Repository Service at National Institute of Oceanography (India)

    Suprit, K.

    A hydrological modelling framework was assembled for simulating discharges of the west-coast rivers of India. The framework is applied to the Mandovi river, Goa, a typical west-coast river. The modelling framework consisted of a digital elevation...

  12. Tidal propagation off the central west coast of India

    Digital Repository Service at National Institute of Oceanography (India)

    Unnikrishnan, A.S.

    Present study reviews the characteristics of tidal propagation on the continental shelf off the central west coast of India. Available observations from tide gauges are used to describe the variations of tidal ranges along the west coast. Amplitudes...

  13. Climate change impacts on fisheries in West Africa: implications for ...

    African Journals Online (AJOL)

    Climate change impacts on fisheries in West Africa: implications for ... as one of the most vulnerable regions to climate change in previous global analyses. ... of the region through economics and food security of West Africa to climate change.

  14. Coeliac disease in children of West Indian origin.

    OpenAIRE

    Hung, J C; Phillips, A D; Walker-Smith, J A

    1995-01-01

    Coeliac disease is uncommon in populations of non-European origin. Two English born West Indian children with coeliac disease are presented. The diagnosis should be considered in children of West Indian origin with chronic diarrhoea.

  15. 78 FR 59841 - Approval and Promulgation of Air Quality Implementation Plans; West Virginia; West Virginia's...

    Science.gov (United States)

    2013-09-30

    ..., disproportionate human health or environmental effects, using practicable and legally permissible methods, under... Environmental protection, Air pollution control, Incorporation by reference, Nitrogen dioxide, Particulate... Environmental Protection, Division of Air Quality, 601 57th Street SE., Charleston, West Virginia 25304....

  16. 77 FR 10423 - Approval and Promulgation of Air Quality Implementation Plans; West Virginia; Amendments to West...

    Science.gov (United States)

    2012-02-22

    ... address, as appropriate, disproportionate human health or environmental effects, using practicable and... preempt tribal law. List of Subjects in 40 CFR Part 52 Environmental protection, Air pollution control...; Amendments to West Virginia's Ambient Air Quality Standards AGENCY: Environmental Protection Agency...

  17. West Coast DA Event data - West Coast Toxic Pseudo-nitzschia bloom

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Beginning in the spring of 2015 the US West Coast began to experience the most wide-spread toxic Pseudo-nitzschia bloom to date, after approximately eight years...

  18. Comparative evaluation of conventional RT-PCR and real-time RT-PCR (RRT-PCR for detection of avian metapneumovirus subtype A Comparação entre as técnicas de RT-PCR convencional e RT-PCR em tempo real para a detecção do metapneumovírus aviários subtipo A

    Directory of Open Access Journals (Sweden)

    Helena Lage Ferreira

    2009-08-01

    Full Text Available Avian metapneumovirus (AMPV belongs to Metapneumovirus genus of Paramyxoviridae family. Virus isolation, serology, and detection of genomic RNA are used as diagnostic methods for AMPV. The aim of the present study was to compare the detection of six subgroup A AMPV isolates (AMPV/A viral RNA by using different conventional and real time RT-PCR methods. Two new RT-PCR tests and two real time RT-PCR tests, both detecting fusion (F gene and nucleocapsid (N gene were compared with an established test for the attachment (G gene. All the RT-PCR tested assays were able to detect the AMPV/A. The lower detection limits were observed using the N-, F- based RRT-PCR and F-based conventional RT-PCR (10(0.3 to 10¹ TCID50 mL-1. The present study suggests that the conventional F-based RT-PCR presented similar detection limit when compared to N- and F-based RRT-PCR and they can be successfully used for AMPV/A detection.O metapneumovírus aviário (AMPV pertence ao gênero Metapneumovirus, família Paramyxoviridae. Isolamento viral, sorologia e detecção do RNA genômico são atualmente as técnicas utilizadas para o diagnóstico desse agente. O objetivo do presente estudo foi comparar a detecção de RNA viral de seis isolados de AMPV, subtipo A (AMPV/A, utilizando diferentes métodos de RT-PCR convencional e real time RT-PCR (RRT-PCR. Duas novas técnicas de RT-PCR convencional e duas técnicas de RRT-PCR, ambas para a detecção dos genes da nucleoproteína (N e da proteína de fusão (F, foram comparadas com um RT-PCR previamente estabelecido para a detecção do AMPV (gene da glicoproteína -G. Todos esses métodos foram capazes de detectar os isolados AMPV/A. As técnicas RRT-PCR (genes F e N mostraram os menores limites de detecção (10(0.3 to 10¹ TCID50 mL-1. Os resultados sugerem que as técnicas RT-PCR convencional (gene F e as técnicas de RRT-PCR (gene F e N desenvolvidas no presente estudo podem ser utilizadas com sucesso para a detecção do

  19. Optimal for difficult roofs. East-west orientation; Optimal fuer schwierige Daecher. Ost-West-Ausrichtung

    Energy Technology Data Exchange (ETDEWEB)

    Bouattour, Mohamed Ali [Solar Frontier Europe GmbH, Muenchen (Germany)

    2013-10-01

    With the help of CIS solar modules the unfavorable east-west roofs can be also use efficiently and profitably, since the good weak-light performance almost compensates the weak light components characterized irradiation conditions. [German] Mit Hilfe der CIS-Solarmodule lassen sich auch die unguenstige Ost-West-Daecher effizient und profitabel nutzen, da das gute Schwachlichtverhalten die hier von schwachen Lichtanteilen gepraegten Einstrahlungsverhaeltnisse nahezu kompensiert.

  20. West Virginia Interpretive Guide Training: A Collaborative Effort

    Science.gov (United States)

    Balcarczyk, Kelly; McKenney, Kathryn; Smaldone, Dave; Arborgast, Doug

    2013-01-01

    West Virginia University's Extension Service partnered with the Recreation, Parks, and Tourism Resources Program to improve guide performance in West Virginia's tourism industry. The result of this partnership is a West Virginia Interpretive Guide Training program aimed at providing low-cost, widely available training to guides…

  1. 78 FR 51199 - West Virginia; Major Disaster and Related Determinations

    Science.gov (United States)

    2013-08-20

    ... SECURITY Federal Emergency Management Agency West Virginia; Major Disaster and Related Determinations... Presidential declaration of a major disaster for the State of West Virginia (FEMA-4132-DR), dated July 26, 2013...''), as follows: I have determined that the damage in certain areas of the State of West...

  2. 75 FR 18517 - West Virginia; Major Disaster and Related Determinations

    Science.gov (United States)

    2010-04-12

    ... SECURITY Federal Emergency Management Agency West Virginia; Major Disaster and Related Determinations... Presidential declaration of a major disaster for the State of West Virginia (FEMA-1893-DR), dated March 29... Act''), as follows: I have determined that the damage in certain areas of the State of West...

  3. 75 FR 25873 - West Virginia; Major Disaster and Related Determinations

    Science.gov (United States)

    2010-05-10

    ... SECURITY Federal Emergency Management Agency West Virginia; Major Disaster and Related Determinations... Presidential declaration of a major disaster for the State of West Virginia (FEMA-1903-DR), dated April 23... Act''), as follows: I have determined that the damage in certain areas of the State of West...

  4. 75 FR 39560 - West Virginia; Major Disaster and Related Determinations

    Science.gov (United States)

    2010-07-09

    ... SECURITY Federal Emergency Management Agency West Virginia; Major Disaster and Related Determinations... Presidential declaration of a major disaster for the State of West Virginia (FEMA-1918-DR), dated June 24, 2010... follows: I have determined that the damage in certain areas of the State of West Virginia resulting...

  5. 77 FR 46103 - West Virginia; Major Disaster and Related Determinations

    Science.gov (United States)

    2012-08-02

    ... SECURITY Federal Emergency Management Agency West Virginia; Major Disaster and Related Determinations... Presidential declaration of a major disaster for the State of West Virginia (FEMA-4071-DR), dated July 23, 2012...''), as follows: I have determined that the damage in certain areas of the State of West...

  6. 77 FR 76061 - West Virginia; Major Disaster and Related Determinations

    Science.gov (United States)

    2012-12-26

    ... SECURITY Federal Emergency Management Agency West Virginia; Major Disaster and Related Determinations... Presidential declaration of a major disaster for the State of West Virginia (FEMA-4093-DR), dated November 27... West Virginia resulting from Hurricane Sandy during the period of October 29 to November 8, 2012, is...

  7. 75 FR 11902 - West Virginia; Major Disaster and Related Determinations

    Science.gov (United States)

    2010-03-12

    ... SECURITY Federal Emergency Management Agency West Virginia; Major Disaster and Related Determinations... Presidential declaration of a major disaster for the State of West Virginia (FEMA-1881-DR), dated March 2, 2010... follows: I have determined that the damage in certain areas of the State of West Virginia resulting from...

  8. Study of West syndrome manifesting periventricular leukomalacia by MRI. Correlation between West syndrome and cerebral white matter lesions

    Energy Technology Data Exchange (ETDEWEB)

    Hamano, Shinichiro; Nara, Takahiro; Shimizu, Masaki; Arita, Jiro; Sakamoto, Masafumi; Eda, Naruyuki; Ogawara, Yoshikazu [Saitama Children`s Medical Center, Iwatsuki (Japan); Maekawa, Kihei

    1995-09-01

    Clinical features of West syndrome manifesting periventricular leukomalacia (PVL) were studied by MRI. The subjects were 10 patients with West syndrome associated with PVL. Occipital spike on electroencephalograms was considered an important characteristic finding of West syndrome. Patients with West syndrome associated with PVL had a lower cerebral blood flow volume at the early phase than those with cryptogenic West syndrome, suggesting a difference of pathophysiology between West syndrome with PVL and cryptogenic West syndrome. Abnormal findings on electroencephalograms and MRI, which are considered to affect the disease course, suggest the presence of cerebral cortical abnormalities, therefore, it is proved to be difficult to study the pathophysiology of West syndrome solely focusing on the findings of the cerebral cortical basal ganglia and the related parts. (Y.S.).

  9. Excerpt from East Is West and West Is East: Gender, Culture, and Interwar Encounters between Asia and America

    OpenAIRE

    Kuo, Karen

    2013-01-01

    Excerpted from Karen Kuo, East Is West and West Is East: Gender, Culture, and Interwar Encounters between Asia and America (Philadelphia: Temple University Press, 2012). Reprinted with permission from Temple University Press.

  10. Excerpt from East Is West and West Is East: Gender, Culture, and Interwar Encounters between Asia and America

    Directory of Open Access Journals (Sweden)

    Karen Kuo

    2013-09-01

    Full Text Available Excerpted from Karen Kuo, East Is West and West Is East: Gender, Culture, and Interwar Encounters between Asia and America (Philadelphia: Temple University Press, 2012.Reprinted with permission from Temple University Press.

  11. West Africa land use and land cover time series

    Science.gov (United States)

    Cotillon, Suzanne E.

    2017-02-16

    Started in 1999, the West Africa Land Use Dynamics project represents an effort to map land use and land cover, characterize the trends in time and space, and understand their effects on the environment across West Africa. The outcome of the West Africa Land Use Dynamics project is the production of a three-time period (1975, 2000, and 2013) land use and land cover dataset for the Sub-Saharan region of West Africa, including the Cabo Verde archipelago. The West Africa Land Use Land Cover Time Series dataset offers a unique basis for characterizing and analyzing land changes across the region, systematically and at an unprecedented level of detail.

  12. The Influence of the West in American Civil War

    Institute of Scientific and Technical Information of China (English)

    张铂

    2016-01-01

    After the 19th century, the West was no longer an appendage of the South and the North. During the Civil War, the North had superiority in both economy and politics. This owed much to the West. The share of the same labour system laid the foundation of the union between the North and the West. The victory of the North linked closely to the support of the West. The essay analyzed the contribution made by the West from the perspectives of economy and politics.

  13. Tectonics in the Northwestern West Philippine Basin

    Institute of Scientific and Technical Information of China (English)

    Ni Xianglong; Wu Shiguo; Shinjo Ryuichi

    2008-01-01

    The West Philippine basin (WPB) is a currently inactive marginal basin belonging to Philippine Sea plate, which has a complex formation history and various crust structures. Based on gravity, magnetic and seismic data, the tectonics in West Philippine basin is characterized by amagnma spreading stage and strike slip fractures. NNE trending Okinawa-Luzon fracture zone is a large fracture zone with apparent geomorphology and shows a right-handed movement. The results of joint gravity-magnetic-seismic inversion suggest that the Okinawa-Luzon fracture zone has intensive deformation and is a transform fault. Western existence of the NW trending fractures under Ryukyu Islands Arc is the main cause of the differences between south and north Okinawa Trough. The Urdaneta plateau is not a remained arc, but remnant of mantle plume although its lava chemistry is similar to oceanic island basalt (OIB).

  14. Evacuation, escape and rescue west of Shetlands

    Energy Technology Data Exchange (ETDEWEB)

    Gallagher, P.; Moran, K.C.

    1998-01-01

    This report has been prepared by WS Atkins Science and Technology on behalf of the Offshore Safety Division of the Health and Safety Executive. It concerns a study into the availability and operability of a range of production platform evacuation and rescue methods which are likely to be used in new field developments in the West of Shetland basin. The research involves an examination of the key factors influencing emergency response arrangements for the West of Shetland sea area. It includes a review of the types of facility envisaged and manning levels, the type of emergency support available to each facility, the availability of helicopters and SAR services. The research also provides comparisons, where appropriate, with both current practice and environmental limitations in the North Sea. (author)

  15. Teaching Scandinavian Interaction Design in West Africa

    DEFF Research Database (Denmark)

    Sørensen, Jannick Kirk

    2016-01-01

    Methods for interaction design have emerged and established themselves first in a Scandinavian context, later in US context and in the rest of the developed world. While good usability and good user experiences are important to all users of ICT, the question is whether the methods and techniques ...... Scandinavian Participatory design can be used to localize the learning process and make interaction design methods sensitive to the West African context. The paper is based on the author’s reflection on his experiences teaching interaction design in West Africa.......Methods for interaction design have emerged and established themselves first in a Scandinavian context, later in US context and in the rest of the developed world. While good usability and good user experiences are important to all users of ICT, the question is whether the methods and techniques...

  16. Aerial view of the West Area complex

    CERN Multimedia

    1976-01-01

    The SPS lies below ground beyond the top of the picture, and the proton beam from it enters the large West Hall from the right. All hadronic beams but one were concentrated in this hall. The neutrino beam from the underground target Passes through BEBC in the tall structure left of centre. The light-coloured building further left houses the neutrino counter experiments WA1 and WA18, and at the left end of the line, in the small block near the edge of the picture sits Gargamelle on a pedestal, since the beam is rising at an angle of about 2 deg. From right to left: West Hall - Bld 180; BEBC - Bld 191; Neutrino - Bld 182; Gargamelle - Bld 185.

  17. West African International Summer School for Young Astronomers

    Science.gov (United States)

    Strubbe, Linda E.; Okere, Bonaventure

    2016-10-01

    The West African International Summer School for Young Astronomers (WAISSYA) is a week-long program for university science students and teachers from West Africa to develop their interest in astronomy. The first summer school was held in Abuja, Nigeria, in 2013; the second Summer School was held in Nsukka, Nigeria, in July 2015. West Africa has a large number of students interested in science, but a paucity of facilities or interest from funding bodies in developing West African astronomy. Our broad goals for the WAISSYA program are: (1) to introduce West African students to astronomy; (2) to exchange ideas about teaching and learning in West Africa and abroad; and (3) to continue building a sustained astronomy partnership between West Africa and Canada. We now briefly describe three defining aspects of WAISSYA 2015.

  18. WEST PIONEER WILDERNESS STUDY AREA, MONTANA.

    Science.gov (United States)

    Berger, Byron R.; Benham, John R.

    1984-01-01

    The West Pioneer Wilderness Study Area is in the Pioneer Mountains, Beaverhead County, Montana. A mineral-resource study of the area identified eight areas with molybdenum potential, four areas with gold-silver potential, one area with tungsten potential, and one area with barite potential. Several small mines were encountered, but none were accessible for the purposes of resource evaluation. No energy resources were identified in the study.

  19. SPS beam to the West Hall

    CERN Multimedia

    1976-01-01

    One of the two target stations feeding the West Hall (see Annual Report 1976). After the proton beam was split into three branches, the outer two were directed on to targets in the cast iron shielding box, the centre one passing through the box to another target station downstream. Five different targets could be put in each beam, controlled by the mechanism seen on top.

  20. Collaborative work between the West and Asia.

    Science.gov (United States)

    Hser, Yih-Ing; Bart, Gavin; Li, Li; Giang, Le Minh

    2013-12-01

    The "Collaborative Work between the West and Asia" session was chaired by Dr. Yih-Ing Hser and had three speakers. The speakers (and their topics) were: Dr. Gavin Bart (Collaborative Addiction Research in Asian Populations Home and Abroad), Dr. Li Li (Implementing Intervention Research Projects in Asia), and Dr. Le Minh Giang (Building Research Infrastructure for International Collaborative Studies on Substance Use Disorder and HIV: The Case of Hanoi Medical University/Vietnam).

  1. WEST COAST REGIONAL CARBON SEQUESTRATION PARTNERSHIP

    Energy Technology Data Exchange (ETDEWEB)

    Larry Myer; Terry Surles; Kelly Birkinshaw

    2004-01-01

    The West Coast Regional Carbon Sequestration Partnership is one of seven partnerships which have been established by the US Department of Energy (DOE) to evaluate carbon dioxide capture, transport and sequestration (CT&S) technologies best suited for different regions of the country. The West Coast Region comprises Arizona, California, Nevada, Oregon, Washington, and the North Slope of Alaska. Led by the California Energy Commission, the West Coast Partnership is a consortium of over thirty five organizations, including state natural resource and environmental protection agencies; national labs and universities; private companies working on CO{sub 2} capture, transportation, and storage technologies; utilities; oil and gas companies; nonprofit organizations; and policy/governance coordinating organizations. In an eighteen month Phase I project, the Partnership will evaluate both terrestrial and geologic sequestration options. Work will focus on five major objectives: (1) Collect data to characterize major CO{sub 2} point sources, the transportation options, and the terrestrial and geologic sinks in the region, and compile and organize this data via a geographic information system (GIS) database; (2) Address key issues affecting deployment of CT&S technologies, including storage site permitting and monitoring, injection regulations, and health and environmental risks (3) Conduct public outreach and maintain an open dialogue with stakeholders in CT&S technologies through public meetings, joint research, and education work (4) Integrate and analyze data and information from the above tasks in order to develop supply curves and cost effective, environmentally acceptable sequestration options, both near- and long-term (5) Identify appropriate terrestrial and geologic demonstration projects consistent with the options defined above, and create action plans for their safe and effective implementation A kickoff meeting for the West Coast Partnership was held on Sept 30-Oct

  2. West Coast, United States and Mexico

    Science.gov (United States)

    1990-01-01

    This view shows the west coast of the United States and Mexico (32.5N, 118.0W) and gives an indication of the range of view from orbital altitude. The visual range of this particular scene is from Skammon's Lagoon on Baja to the northern tip of California's Central Valley and Sierra Nevada, a range of over 15 degrees of latitude. Coastal fog drapes over southern California and northern Baja California. White Sands, New Mexico is at far right center.

  3. Developing Pipeline Transportation in West China

    Institute of Scientific and Technical Information of China (English)

    Yang Chenghan; Wang Wei

    1997-01-01

    @@ Since the late 1980s, focus of exploration and development for oil & gas has been diverted to thewest of China, resulting in the discovery and development of Shaanbei gas field followed by large-scale exploration and development of Tarim,Turpan and Hami basins. Responding to this situation, pipeline construction focus has also been transferred to west China where large-scale development of pipeline transportation, an opportunity as well as a challenge, is expected.

  4. Electrometallurgical treatment demonstration at ANL-West

    Energy Technology Data Exchange (ETDEWEB)

    Goff, K. M.; Benedict, R. W.; Johnson, S. G.; Mariani, R. D.; Simpson, M. F.; Westphal, B. R.

    2000-03-20

    Electrometallurgical treatment (EMT) was developed by Argonne National Laboratory (ANL) to ready sodium-bonded spent nuclear fuel for geological disposal. A demonstration of this technology was successfully completed in August 1999. EMT was used to condition irradiated EBR-II driver and blanket fuel at ANL-West. The results of this demonstration, including the production of radioactive high-level waste forms, are presented.

  5. Visual examinations of K west fuel elements

    Energy Technology Data Exchange (ETDEWEB)

    Pitner, A.L., Fluor Daniel Hanford

    1997-02-03

    Over 250 fuel assemblies stored in sealed canisters in the K West Basin were extracted and visually examined for damage. Substantial damage was expected based on high cesium levels previously measured in water samples taken from these canisters. About 11% of the inner elements and 45% of the outer elements were found to be failed in these examinations. Canisters that had cesium levels of I curie or more generally had multiple instances of major fuel damage.

  6. The Ramsar Sites of Disko, West Greenland

    DEFF Research Database (Denmark)

    Egevang, C.; Boertmann, D.

    The three Ramsar sites of Disko Island in West Greenland were surveyed for breeding and staging waterbirds in July 2001. Two of the areas (no. 1 and 2) held a high diversity of waterbirds and proved to be of international importance for the Greenland white-fronted goose, while the third (no. 3......) held very few waterbirds and hardly meet any of the specific waterbird criteria of the Ramsar convention...

  7. Multiculturalism and Minority Rights: West and East

    Directory of Open Access Journals (Sweden)

    Will Kymlicka

    2002-10-01

    Full Text Available Are Western models of multiculturalism and minority rights relevant for the post-Communist countries of Central and Eastern Europe? This article describes a range of Western models, and explores the social and political conditions that have led to their adoption in the West. It then considers various factors which might make the adoption of these models difficult in Eastern Europe, and considers the potential role of the international community in overcoming these obstacles.

  8. IR thermography diagnostics for the WEST project

    Energy Technology Data Exchange (ETDEWEB)

    Courtois, X., E-mail: xavier.courtois@cea.fr [CEA, IRFM, F-13108 Saint-Paul-Lez-Durance (France); Aumeunier, M.H. [OPTIS, ZE de La Farlède, F-83078 Toulon Cedex 9 (France); Joanny, M.; Roche, H.; Micolon, F.; Salasca, S.; Balorin, C.; Jouve, M. [CEA, IRFM, F-13108 Saint-Paul-Lez-Durance (France)

    2014-10-15

    Highlights: • The WEST project requires a set of three infrared diagnostics. • The tungsten divertor will be monitored by the existing diagnostic renewed. • The antennas monitoring require the development of an innovative diagnostic. • A fiber bundle will be used as image transport for the antennas monitoring. • A wide angle tangential view of the upper divertor and the first wall is studied. - Abstract: To operate long plasma discharge in tokamak equipped with actively cooled plasma facing components (PFC), infrared (IR) thermography is a key diagnostic. Indeed IR data are used for both PFC safety monitoring, to avoid material degradation and water leak, and various physics studies on plasma-wall interaction. The IR monitoring is becoming even more crucial with today metallic PFCs. This is the case for the WEST project (Tungsten (W) Environment for Steady State Tokamak), which aims at installing a W divertor in Tore Supra (TS), in order to operate the 1st tokamak with a full W actively cooled divertor in long plasma discharges. The IR thermography system for the WEST project described in this paper will consist of a set of 3 different diagnostics: (1) Six cameras located in upper ports viewing the full W divertor, which reuse a part of the existing diagnostic of TS. (2) Five novel views located behind the inner protection panels for the antennas monitoring, based on an innovative imaging fibers bundle technology. (3) A tangential wide angle view located in a median port, for the upper divertor and first wall monitoring.

  9. Cause of East-West Earth Asymmetry

    CERN Document Server

    Scalera, Giancarlo

    2015-01-01

    The different slope of the Wadati-Benioff zones oriented towards east and west is considered a main asymmetry of the Earth's globe. Under the Americas they have angles of about 30o, while under the Pacific east coasts (Asia, Japan) the angles are steeper. In the framework of plate tectonics geodynamics the cause of this difference can be identified in the tidal drag that would cause a global shift of the lithosphere towards west. But this solution has been many times criticized on the basis of the irrelevance of the tidal forces with respect to viscous friction. Instead, it is possible to show that in a different framework, in which sudden extrusions of mantle materials occur by local phase change toward a more unpacked lattice, the value of the Coriolis fictitious force can rise of several magnitude orders, becoming the main cause of the east-west asymmetry of the Wadati-Benioff zones, which might be ascribed entirely to internal causes of the planet (its rotation and geodynamics) and not to external causes ...

  10. History and the West Indian nation

    Directory of Open Access Journals (Sweden)

    Frank Birbalsingh

    1998-07-01

    Full Text Available [First paragraph] The Art of Kamau Brathwaite. STEWART BROWN (ed.. Bridgend, Wales: Seren/Poetry Wales Press, 1995. 275 pp. (Cloth US$ 50.00, Paper US$ 22.95 Atlantic Passages: History, Community, and Language in the Fiction of Sam Selvon. MARK LOOKER. New York: Peter Lang, 1996. x + 243 pp. (Cloth n.p. Caliban's Curse: George Lamming and the Revisioning of History. SUPRIYA NAIR. Ann Arbor: University of Michigan Press, 1996. viii + 171 pp. (Cloth US$ 34.50 Phyllis Shand Allfrey: A Caribbean Life. LlZABETH PARAVISINI-GEBERT. New Brunswick NJ: Rutgers University Press, 1996. xii + 335 pp. (Cloth US$ 55.00, Paper US$ 18.95 Of the four books to be considered here, those on Brathwaite, Selvon, and Lamming fit snugly together into a natural category of literature that has to do with the emergence of a Creole or African-centered Caribbean culture, and related issues of race, color, class, history, and nationality. The fourth is a biography of Phyllis Shand Allfrey, a white West Indian, who is of an altogether different race, color, and class than from the other three. Yet the four books are linked together by nationality, for Allfrey and the others are all citizens of one region, the English-speaking West Indies, which, as the Federation of the West Indies between 1958 and 1962, formed a single nation.

  11. Poverty and health in West Germany.

    Science.gov (United States)

    Helmert, U; Mielck, A; Shea, S

    1997-01-01

    The relationship between poverty and several health-related characteristics in West Germany was investigated. Data were derived from the National and Regional Health Surveys conducted in West Germany from 1984 to 1992. 25,544 males and 25,719 females with German nationality aged 25-69 years were examined. Poverty was defined as a household income of 50% less than the mean for West Germany. Multiple logistic regression analysis was used to analyze the relationship between poverty and four health variables: individual health behavior, subjective assessment of health status, cardiovascular disease risk factors, and self-reported prevalence of lifetime chronic diseases. 10.2% of males and 12.8% of females were classified as being below the poverty line. For most but not all health parameters, less favourable results were found for the segment of the population with a household income below the poverty line. The most striking poverty-related differences were observed for lack of regular sport activities, subjective health satisfaction, obesity and myocardial infarction/stroke. Significantly lower prevalence rates for study subjects below the poverty line were observed for hypercholesterolemia in females only. Allergic disorders were the only chronic diseases reported significantly less often in males and females below the poverty line. Poverty has strong effects on individual health status and the prevalence of chronic diseases. Due to the rising unemployment rates in Germany in the last years it is very likely that the strong negative consequences of poverty for health are increasing.

  12. Enhanced integrators for WEST magnetic diagnostics

    Energy Technology Data Exchange (ETDEWEB)

    Spuig, Pascal, E-mail: pascal.spuig@cea.fr [CEA IRFM, F-13108 Saint-Paul-lez-Durance (France); Kumari, Praveena [Institute for Plasma Research (IPR), Bhat, Gandhinagar District 382428 (India); Moreau, Michel; Moreau, Philippe; Le-luyer, Alain; Malard, Philippe [CEA IRFM, F-13108 Saint-Paul-lez-Durance (France)

    2015-10-15

    Highlights: • On Tokamaks, analogue integrators are key instruments. • Our analogue integrators is reliable and fast, thus relevant for fusion facilities. • Our integrator fulfils requirements for long time pulses of WEST Tokamak. • The performances are amongst the best never obtained for analogue integrators. - Abstract: The WEST project consists of implementing a divertor configuration in the Tore Supra Tokamak. This modification requires to renew the magnetic diagnostics and especially adding new magnetic sensors in the divertor region. This has provided an opportunity to consider some enhancements to the existing integrator modules of the magnetic diagnostics. Two prototypes have been produced using different combinations of technical solutions, aiming to achieve lower drift and higher common mode rejection ratio (CMRR). This paper presents the explored and tested ways we followed in order to create enhanced analogue integrators for long pulses. The results of extensive and comprehensive tests are summarized. The achieved performances are analyzed regarding the WEST project specifications and furthermore, the latest ITER requirements for magnetic diagnostics integrators are also considered.

  13. The ARM West Antarctic Radiation Experiment (AWARE)

    Science.gov (United States)

    Lubin, Dan; Bromwich, David; Vogelmann, Andrew; Verlinde, Johannes; Russell, Lynn

    2016-04-01

    West Antarctica is one of the most rapidly warming regions on Earth, and its changing climate in both atmosphere and ocean is linked to loss of Antarctic ice mass and global sea level rise. The specific mechanisms for West Antarctic Ice Sheet (WAIS) warming are not fully understood, but are hypothesized to involve linkage between moisture from Southern Ocean storm tracks and the surface energy balance over the WAIS, and related teleconnections with subtropical and tropical meteorology. This present lack of understanding has motivated a climate science and cloud physics campaign jointly supported by the US National Science Foundation (NSF) and Department of Energy (DOE), called the Atmospheric Radiation Measurement Program (ARM) West Antarctic Radiation Experiment (AWARE). The DOE's second ARM Mobile Facility (AMF2) was deployed to McMurdo Station on Ross Island in November 2015 and will operate through December 2016. The AMF2 includes (1) cloud research radars, both scanning and zenith, operating in the Ka- and X-bands, (2) high spectral resolution and polarized micropulse lidars, and (3) a suite of shortwave and longwave broadband and spectral radiometers. A second suite of instruments is deployed at the WAIS Divide Ice Camp on the West Antarctic plateau during December 2015 and January 2016. The WAIS instrument suite provides (1) measurement of all surface energy balance components, (2) a polarized micropulse lidar and shortwave spectroradiometer, (3) microwave total water column measurement, and (4) four times daily rawinsonde launches which are the first from West Antarctica since 1967. There is a direct linkage between the WAIS instrument suite and the AMF2 at McMurdo, in that air masses originating in Southern Ocean storm tracks that are driven up over the WAIS often subsequently descend over the Ross Ice Shelf and arrive at Ross Island. Preliminary data are already illustrating the prevalence of mixed-phase clouds and their role in the surface energy balance

  14. The ARM West Antarctic Radiation Experiment (AWARE)

    Science.gov (United States)

    Scott, R. C.; Lubin, D.; Bromwich, D. H.; Vogelmann, A. M.; Verlinde, J.; Russell, L. M.

    2016-12-01

    West Antarctica is one of the most rapidly warming regions on Earth, and its changing climate in both atmosphere and ocean is linked to loss of Antarctic ice mass and global sea level rise. The specific mechanisms for West Antarctic Ice Sheet (WAIS) warming are not fully understood, but are hypothesized to involve linkage between moisture from Southern Ocean storm tracks and the surface energy balance over the WAIS, and related teleconnections with subtropical and tropical meteorology. This present lack of understanding has motivated a climate science and cloud physics campaign jointly supported by the US National Science Foundation (NSF) and Department of Energy (DOE), called the Atmospheric Radiation Measurement Program (ARM) West Antarctic Radiation Experiment (AWARE). The DOE's second ARM Mobile Facility (AMF2) was deployed to McMurdo Station on Ross Island in November 2015 and will operate through December 2016. The AMF2 includes (1) cloud research radars, both scanning and zenith, operating in the Ka- and X-bands, (2) high spectral resolution and polarized micropulse lidars, and (3) a suite of shortwave and longwave broadband and spectral radiometers. A second suite of instruments is deployed at the WAIS Divide Ice Camp on the West Antarctic plateau during December 2015 and January 2016. The WAIS instrument suite provides (1) measurement of all surface energy balance components, (2) a polarized micropulse lidar and shortwave spectroradiometer, (3) microwave total water column measurement, and (4) four times daily rawinsonde launches which are the first from West Antarctica since 1967. There is a direct linkage between the WAIS instrument suite and the AMF2 at McMurdo, in that air masses originating in Southern Ocean storm tracks that are driven up over the WAIS often subsequently descend over the Ross Ice Shelf and arrive at Ross Island. Preliminary data are already illustrating (1) the prevalence of single- versus mixed-phase clouds and their role in the

  15. Profiles Junior high School West Java in Education Learning Environment

    Directory of Open Access Journals (Sweden)

    NFN Nahadi

    2014-02-01

    Full Text Available Descriptive studies have been conducted on the existing junior high profile in West Java on Education Learning Environment. The study was conducted by purposive sampling and descriptive done to get an idea about the profile of SMP in West Java implementation of the learning environment. in junior high school in West Java. Research conducted by distributing questionnaires, and observations based on the indicators developed. Based on this research, it is known that, PLH learning in junior high school in West Java has been in force since 2007 after the enactment of the Governor of West Java on environmental education for junior high school students in West Java. Learning that lasts generally implemented in conventional teacher, and it is without any innovations. It has made learning the essential condition runs well, but has not lasted optimally.

  16. Raw material studies of West Central Serbia

    Directory of Open Access Journals (Sweden)

    Vera Bogosavljević Petrović

    2014-03-01

    Full Text Available This paper deals with raw material problems in the territory of West Central Serbia geologically determined as the Čačak-Kraljevo (or West Morava basin. Our research is presented through the most striking case studies, Lojanik, Vlaška Glava and Lazac.  The Lojanik hill is a silicified forest by origin. It has occasionally been in use from the earliest periods of prehistory until today as a source of black and ochre-coloured flint, opal and silicified wood. A detailed prospection, including the mapping of surface finds using square nets, was conducted during two research campaigns.The Vlaška Glava is an open-air Palaeolithic site at which artefacts made of white, ochre, red, brown and black chert, silicified magnesite, volcanic and metamorphic rocks were found. Our research of primary and secondary geological deposits in the vicinity of the site showed equivalent raw material. We also found an interesting primary deposit of high quality bluish grey flint with outcrop activities (Workshop 1.The Lazac shaft is a contemporary magnesite mine, recently abandoned because of the high percentage of silicon-dioxide. We determined the same raw material in collections found at nearby Neolithic sites. Certain similarities between the wooden support systems of ore exploration in the Middle Ages and modern times were established at the entrance of the shaft.Our research in the territory of the West Morava basin resulted in reconstruction of some links between geological deposits and settlements and also creation of a relevant base for future raw material studies.

  17. Lassa fever: another threat from West Africa.

    Science.gov (United States)

    Brosh-Nissimov, Tal

    2016-01-01

    Lassa fever, a zoonotic viral infection, is endemic in West Africa. The disease causes annual wide spread morbidity and mortality in Africa, and can be imported by travelers. Possible importation of Lassa fever and the potential for the use of Lassa virus as an agent of bioterrorism mandate clinicians in Israel and other countries to be vigilant and familiar with the basic characteristics of this disease. The article reviews the basis of this infection and the clinical management of patients with Lassa fever. Special emphasis is given to antiviral treatment and infection control.

  18. Wild snakes harbor West Nile virus

    Directory of Open Access Journals (Sweden)

    C.R. Dahlin

    2016-12-01

    Full Text Available West Nile virus (WNV has a complex eco-epidemiology with birds acting as reservoirs and hosts for the virus. Less well understood is the role of reptiles, especially in wild populations. The goal of our study was to determine whether a wild population of snakes in Pennsylvania harbored WNV. Six species of snakes were orally sampled in the summer of 2013 and were tested for the presence of WNV viral RNA using RT-PCR. Two Eastern Garter Snakes, Thamnophis sirtalis sirtalis tested positive for viral RNA (2/123, 1.62%. These results indicate a possible role for snakes in the complex transmission cycle of WNV.

  19. East-West cooperation at CERN

    CERN Multimedia

    2002-01-01

    From left to right: H. Bokemeyer, in charge of physics for INTAS, J. Sinnaeve, INTAS' general secretary and R. Landua, ATHENA spokesman, visit the ATHENA experiment installations. Heads of INTAS (International Association for the promotion and cooperation of the new independent states of the former Soviet Union) visited CERN on 11 October. This association is in charge of preserving and promoting the scientific potential of the former Soviet Union countries through a cooperation between East and West. In recents years, a certain number of projects related to the LHC experiments have been co-financed by INTAS. The support for young researchers coming from these countries is also a big success.

  20. West of Shetlands FPSO or TLP

    Energy Technology Data Exchange (ETDEWEB)

    Anon.

    1995-09-01

    One of the most promising and active areas, in terms of exploration, for hydrocarbon production offshore Europe is located West of the Shetland Islands and lies in water depths ranging from 350 m to 900 m subject to an extremely harsh environment. Three different field-proven concepts from the Doris Engineering`s point of view must be considered for this deep water area: a floating Production Storage and Off-loading (FPSO) unit, a production Tension Leg Platform (TLP) and a mini TLP designed to support wellheads and work aver facility. (author). 1 fig.

  1. Orchids inventory in Sintang Regency, West Kalimantan

    Directory of Open Access Journals (Sweden)

    ESTI ENDAH ARIYANTI

    2008-10-01

    Full Text Available Orchid is one of ornamental plants which have commercial value. Therefore most species are becoming threatened or even endangered because of over exploitation. In addition, its natural habitat is also decreasing. Conservation must be done urgently, both by in situ and ex situ conservation, which can be started by orchid inventory. The orchid inventory was done in TWA Bukit Kelam, TWA Baning and several places in Regency of Sintang, West Kalimantan. The result showed that there were 40 species belonged to 27 genera, which 32 species of them (20 genera were epiphytic orchids and 8 species (7 genera were terrestrial orchids.

  2. 33 CFR 110.189a - Key West Harbor, Key West, Fla., naval explosives anchorage area.

    Science.gov (United States)

    2010-07-01

    ..., Key West, Fla., naval explosives anchorage area. (a) The anchorage ground. A circular area with its center at latitude 24°30′50.6″, longitude 81°50′31.6″ with a radius of 300 yards, for use for ammunition exceeding the prescribed limits for pier-side handling. (b) The regulations. (1) When occupied by a...

  3. West Virginia Digital Learning: Report to the Governor, Legislature, and West Virginia Board of Education

    Science.gov (United States)

    Alliance for Excellent Education, 2014

    2014-01-01

    Accomplishing personalized, deeper learning through anywhere, anytime digital learning requires a redesign of the K-12 education system. This report looks at readiness for digital learning at two levels in West Virginia: the district capacity building to ready the system for digital learning and school implementation of digital learning. The…

  4. New emerging West Africa Ebola 2014:the present global threaten

    Institute of Scientific and Technical Information of China (English)

    Viroj Wiwanitkit

    2014-01-01

    New emerging West Africa Ebola 2014 is the present global threaten. It is a new emerging viral infection that primarily occurred in West Africa and poses the possible trend of worldwide pandemic. The 2014 West Africa Ebola outbreak is the most severe in recorded history in regards to both the number of human cases and fatalities. World Health Organization calls for global concern and attempts to stop the spread of this emerging viral infection. In this brief review, the author presents and discusses on the clinical feature of the new emerging West Africa Ebola 2014.

  5. K West integrated water treatment system subproject safety analysis document

    Energy Technology Data Exchange (ETDEWEB)

    SEMMENS, L.S.

    1999-02-24

    This Accident Analysis evaluates unmitigated accident scenarios, and identifies Safety Significant and Safety Class structures, systems, and components for the K West Integrated Water Treatment System.

  6. New emerging West Africa Ebola 2014: the present global threaten

    Directory of Open Access Journals (Sweden)

    Viroj Wiwanitkit

    2014-07-01

    Full Text Available New emerging West Africa Ebola 2014 is the present global threaten. It is a new emerging viral infection that primarily occurred in West Africa and poses the possible trend of worldwide pandemic. The 2014 West Africa Ebola outbreak is the most severe in recorded history in regards to both the number of human cases and fatalities. World Health Organization calls for global concern and attempts to stop the spread of this emerging viral infection. In this brief review, the author presents and discusses on the clinical feature of the new emerging West Africa Ebola 2014.

  7. Empowerment model of biomass in west java

    Science.gov (United States)

    Mulyana, C.; Fitriani, N. I.; Saad, A.; Yuliah, Y.

    2017-06-01

    Scarcity of fossil energy accelerates the search of renewable energy sources as the substitution. In West Java, biomass has potential to be developed into bio-briquette because the resources are abundant. The objectives of this research are mapping the potency of biomass as bio-briquette in West Java, and making the model of the empowerment biomass potential involving five fundamental step which are raw material, pre-processing process, conversion mechanism, products, and end user. The main object of this model focused on 3 forms which are solid, liquid, and gas which was made by involving the community component as the owner biomass, district government, academics and researcher communities, related industries as users of biomass, and the central government as the policy holders and investors as a funder. In the model was described their respective roles and mutual relationship one with another so that the bio-briquette as a substitute of fossil fuels can be realized. Application of this model will provide the benefits in renewability energy sources, environmental, socio economical and energy security.

  8. Architecture of WEST plasma control system

    Energy Technology Data Exchange (ETDEWEB)

    Ravenel, N., E-mail: nathalie.ravenel@cea.fr [CEA, IRFM, F-13108 Saint-Paul-lez-Durance (France); Nouailletas, R.; Barana, O.; Brémond, S.; Moreau, P.; Guillerminet, B.; Balme, S.; Allegretti, L. [CEA, IRFM, F-13108 Saint-Paul-lez-Durance (France); Mannori, S. [ENEA C.R. Brasimone (Italy)

    2014-05-15

    To operate advanced plasma scenario (long pulse with high stored energy) in present and future tokamak devices under safe operation conditions, the control requirements of the plasma control system (PCS) leads to the development of advanced feedback control and real time handling exceptions. To develop these controllers and these exceptions handling strategies, a project aiming at setting up a flight simulator has started at CEA in 2009. Now, the new WEST (W Environment in Steady-state Tokamak) project deals with modifying Tore Supra into an ITER-like divertor tokamak. This upgrade impacts a lot of systems including Tore Supra PCS and is the opportunity to improve the current PCS architecture to implement the previous works and to fulfill the needs of modern tokamak operation. This paper is dealing with the description of the architecture of WEST PCS. Firstly, the requirements will be presented including the needs of new concepts (segments configuration, alternative (or backup) scenario, …). Then, the conceptual design of the PCS will be described including the main components and their functions. The third part will be dedicated to the proposal RT framework and to the technologies that we have to implement to reach the requirements.

  9. West Virginia's Lost Youth: Appalachian Stereotypes and Residential Preferences

    Science.gov (United States)

    Towers, George

    2005-01-01

    This study uses a cognitive mapping survey to examine the effect of Appalachian stereotypes on West Virginia high school students' residential preferences. The research addresses the popularly held hypothesis that West Virginia is suffering out-migration of its young people in part because of negative regional imagery. Survey results provide some…

  10. Forest gradients in West Africa. A spatial gradient analysis.

    NARCIS (Netherlands)

    Rompaey, van R.S.A.R.

    1993-01-01

    The tropical rain forests of West Africa, west of the Dahomey interval, once covered some 40 million ha. Being on the western fringe of the African continent, they receive abundant rainfall from the SW monsoon. Further inland, rainfall gradually decreases and the forests give way to savanna and ulti

  11. Forest gradients in West Africa : a spatial gradient analysis

    NARCIS (Netherlands)

    Rompaey, van R.S.A.R.

    1993-01-01

    The tropical rain forests of West Africa, west of the Dahomey interval, once covered some 40 million ha. Being on the western fringe of the African continent, they receive abundant rainfall from the SW monsoon. Further inland, rainfall gradually decreases and the forests give way to savanna and

  12. A Romantic Study of Shelly's "Ode to the West Wind"

    Institute of Scientific and Technical Information of China (English)

    冯敏贤

    2007-01-01

    Ode to the West Wind is one of the marvelous poetry of Percy Bysshe Shelley. In order to study the Romanticism in Shelly's Ode to the West Wind, this thesis, by studying the features of Romanticism, shows the fascinating romantic beauty and glamour of this poetry.

  13. Dateline USA: Settling the West, 1800-1900.

    Science.gov (United States)

    Chatton, Barbara; O'Laughlin, Michael

    1998-01-01

    Presents an annotated bibliography of titles related to the theme "Settling the West" which are appropriate for primary, elementary, and middle grades. Topics include overviews of Western history; early 19th century; the gold rush; the pony express; travelers by trail, rail, and ship; settling the West; cowboys and cattle; and the…

  14. W.E.S.T. Pilot Project. Final Report.

    Science.gov (United States)

    O'Gorman, Lori A. Stinson

    The Saskatchewan Federation of Labor (SFL) conducted a Workers' Education for Skills Training, or WEST Program, to upgrade its membership's basic literacy skills. The purpose of the WEST Program was to develop, ensure access to, and build support for a workplace literacy program designed to meet the diverse needs of affiliates' members. Six…

  15. Energy Generation Potential of West African Ocean Current ...

    African Journals Online (AJOL)

    PROF. OLIVER OSUAGWA

    2015-12-01

    Dec 1, 2015 ... Department of Marine Engineering, Faculty of Technology, Federal University of Petroleum Resources, P.M.B. 1221 ... West Africa in a streamed regular supply. ... system; Design of ocean current generating system whose efficiency relies .... Offloading Liquefied Natural Gas Facility for Offshore West Africa.

  16. The Contrast between Festivals in China and In West

    Institute of Scientific and Technical Information of China (English)

    乔烨辛; 乌日乐

    2013-01-01

    When we study a kind of language,it is important to know the phonetic culture background first.We are going to discuss the traditional festivals’origins,social background,and festival customs in China and in West.China has the festal likeness in representative tradition with west the place of the difference.

  17. BP Announces Withdrawal from West-to-East Gas Project

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    @@ On August 31, 2001, BP announced that BP would not participate in the bidding launched by PetroChina for the West-to-East gas transmission project,China's landmark project for West Development that is valued billions of US dollars.

  18. West Virginia's Lost Youth: Appalachian Stereotypes and Residential Preferences

    Science.gov (United States)

    Towers, George

    2005-01-01

    This study uses a cognitive mapping survey to examine the effect of Appalachian stereotypes on West Virginia high school students' residential preferences. The research addresses the popularly held hypothesis that West Virginia is suffering out-migration of its young people in part because of negative regional imagery. Survey results provide some…

  19. The German East-West Divide in Knowledge Production

    DEFF Research Database (Denmark)

    Patuelli, Roberto; Vaona, Andrea; Grimpe, Christoph

    2010-01-01

    , in particular between the East and the West. Based on analyses at the NUTS 3 regional level, we find different knowledge production functions for the East and the West. Moreover, we investigate how our results are affected by the adoption of alternative aggregation levels. Overall, our findings suggest...

  20. 77 FR 20044 - West Virginia; Major Disaster and Related Determinations

    Science.gov (United States)

    2012-04-03

    ... SECURITY Federal Emergency Management Agency West Virginia; Major Disaster and Related Determinations... Presidential declaration of a major disaster for the State of West Virginia (FEMA-4059-DR), dated March 16... ``Stafford Act''), as follows: I have determined that the damage in certain areas of the State of...

  1. 77 FR 20042 - West Virginia; Major Disaster and Related Determinations

    Science.gov (United States)

    2012-04-03

    ... SECURITY Federal Emergency Management Agency West Virginia; Major Disaster and Related Determinations... Presidential declaration of a major disaster for the State of West Virginia (FEMA-4061-DR), dated March 22... ``Stafford Act''), as follows: I have determined that the damage in certain areas of the State of...

  2. Marine West Coast Forests, Chapter 9

    Science.gov (United States)

    Perakis, Steven S.; Geiser, Linda H.; Lilleskov, Erik A.; Pardo, Linda H.; Robin-Abbott, Molly J.; Driscoll, Charles T.

    2011-01-01

    Human activities have greatly increased nitrogen emissions and deposition across large areas of Earth. Although nitrogen is an essential nutrient for plant growth, too much nitrogen in excess of critical loads leads to losses of biodiversity, soil and stream acidification, nutrient imbalances, and other deleterious effects. In a new report quantifying critical loads of nitrogen deposition across the United States, USGS scientist Steve Perakis and co-authors provided a chapter about responses of marine west coast forests. Much of this region is understudied with respect to nitrogen deposition, and in this chapter the authors identify known adverse effects and estimate critical loads of nitrogen deposition for western Oregon and Washington and southeast Alaska forests. Perakis also contributed to the synthesis chapter, which includes background, objectives, advantages and uncertainties of critical loads, an overview of critical loads across U.S. ecoregions, and other topics.

  3. Usability and Interaction Design in West Africa

    DEFF Research Database (Denmark)

    Sørensen, Jannick Kirk

    , with a specific aim at the dissemination of knowledge in field of interaction design both in an academic context, as well as among practitioners. More specifically the paper discusses how interaction design knowledge can be disseminated in West Africa, particularly in Higher Education.......Good usability is important in all ICT solutions. To achieve good usability, a good praxis for interaction design is needed. Usability and interaction design have however emerged and established itself in a North European and US context. The ICT industry in Africa do not have the same resources...... in the field of interaction design as in the developed world. While good usability and good user experiences are important to all users of ICT, the question is whether the methods and techniques that were mainly developed in Scandinavia, Europe and US are suitable for ICT development in Africa? Can ideals...

  4. I Chemistry Conference of West Bahia

    Directory of Open Access Journals (Sweden)

    Boaz Galdino de Oliveira

    2016-02-01

    Full Text Available The I Chemistry Conference of West Bahia [1] is an event carried out by the Núcleo de Ensino, Pesquisa e Extensão de Química (NEPEQ, and aims to disseminate chemical researches developed on the Universidade Federal do Oeste da Bahia (UFOB at undergraduate level as well as on the master’s program. This special issue contains some of works presented throughout the JornaQui, such as those from analytical chemistry, natural products, theoretical chemistry, medicinal chemistry, chemistry teaching, spectroscopy, materials science, catalysis and photochemistry. In this context, we would like to highlight the researches of bioactive compounds is by molecular modeling or phytochemistry, studies of intermolecular systems in the infrared spectrum, environmental monitoring by using experimental techniques, new nanocompounds characterized by X-ray diffraction, photochemical degradation organic material, for example. DOI: http://dx.doi.org/10.17807/orbital.v1i1.844

  5. November 2016 West Hackberry Subsidence Report

    Energy Technology Data Exchange (ETDEWEB)

    Moriarty, Dylan Michael [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Lord, Anna C. Snider [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2017-02-01

    Subsidence monitoring is a critical component to understanding the cavern integrity of salt storage caverns. This report looks at historical and recent data from two of the three West Hackberry dome cavern operators. DOE SPR and LA Storage are coordinating subsidence surveys to create a comprehensive understanding of ground movement above the dome. Data from annual level and rod surveys, GPS, and tiltmeter data show the sites are experiencing typical ground movement. The highest subsidence rate is seen in the middle of the DOE SPR site at just under one inch per year with less ground movement around the edge of the site. A GPS and tiltmeter instrument in the northeast areas of the DOE SPR site has not seen any trend change since the devices were installed in 2013. Comparison between recent ground movement data and historical trends suggest that there is no reason to believe that any DOE SPR or LA Storage caverns have been structurally compromised.

  6. ’The West Is Polluting China’

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    China is under increasingly severe criticism from the West for its environ- menial problems,but recently there are varying voices in the international community about the reasons surrounding this issue.According to Britain’s New Economics Foundation,Western countries rely heavily on Chinese commodities,meaning in the process of manufacturing they are transferring pollution to China.This London-based think tank also puts forward that the discussion should shift its focus from commodity producers to consumers. Writing in the Economic Observer,Professor Hou Dongmin from the Center for Population and Development Studies under the Renmin University of China says that the international community has to face up to the truth.He believes one of the reasons for environmental pollution in China is the country is undertaking a high proportion of global manufacturing activities,which naturally brings environmental pressure to China.Excerpts of Hou’s thoughts follow:

  7. Optical polarimetry of Comet West 1976 VI

    Energy Technology Data Exchange (ETDEWEB)

    Michalsky, J.J.

    1981-09-01

    Four narrowband filters covering the 440-850 nm wavelengths were used to measure the polarization of the continuum of Comet West 1976 VI. Postperihelion observations indicated wavelength-independent linear polarization in all of the three measurements made, in agreement with other polarization measurements of the comet from the visible to the near-infrared but in contrast with the general tendency in comets toward polarization increase with wavelength. No circular polarization was detected, and it is suggested by (1) the magnitude of polarization as a function of scattering angle, (2) wavelength independence, and (3) infrared and optical photometric properties, that dirty silicates with radii approaching 5 microns may be responsible for the phenomena observed.

  8. Spectrophotometry of Comet West 1976 VI

    Energy Technology Data Exchange (ETDEWEB)

    Rozenbush, V.K.

    1986-05-01

    Spectra obtained for the coma, nuclear fragments A and D, and tail of Comet West 1976 VI on April 1, 2 and 7, 1976 are noted to encompass coma spectra which differed from those of the nuclear fragments, which exhibited a strong continuum with superimposed emissions that included a stronger CO(+)-band system than that of the coma. A detailed comparison between fragment spectra has revealed great differences in both quasi-simultaneously obtained and five-day-separated cases. The relative intensities of different CO(+) bands are compared to the theoretical ones, and the abundances of CO(+) ions corresponding to different vibrational transitions are determined relative to that of CN. 24 references.

  9. [Epidemics and colonial medicine in West Africa].

    Science.gov (United States)

    Becker, C; Collignon, R

    1998-01-01

    We studied colonial medical practices and health policies in West Africa, which has faced endemics and epidemics that have affected entire societies. We found that attempts to centralize the organization of colonial medicine, which began in the late 19th Century, were limited until the end of World War I. Research and control programs expanded after 1920, concentrating on epidemics, but largely ignoring many major health problems, such as measles, whooping cough and malnutrition, the importance of which were not recognized until after World War II. This appraisal of colonial medicine highlights the difficulties of introducing modern medicine and its ideas into African societies. There have been various social reactions, resulting in the current duality in which traditional and modern medicine coexist.

  10. Dry spell frequency in West Java, Indonesia

    Science.gov (United States)

    Purnaningtyas, V. I.; Boer, R.; Faqih, A.

    2017-03-01

    The climatology and variability of dry spells are valuable information for scientists, engineers, planners, and managers working in water-related sectors such as agriculture, ecology, hydrology, and water resources. The dry spell concerns consecutive dry days which are the largest number of consecutive days with less than 1 mm of daily precipitation within a year. The objective of this study is to analyse the spatial and temporal characteristics of dry spells and also specifically investigate the frequency patterns of the dry spell distribution based on historical observed daily precipitation from 1981 to 2010. The longest dry spell occured at Pasirukem with the length of 252 days. The northern coast of West Java have higher probabilities of dry spells more than 5, 10, 15, and 20 days.

  11. BRYOPHYTES OF MOUNT PATUHA, WEST JAVA, INDONESIA

    Directory of Open Access Journals (Sweden)

    ROBBERT GRADSTEIN

    2010-11-01

    Full Text Available GRADSTEIN, R.    et al. 2010. Bryophytes of Mount Patuha, West  Java,  Indonesia.  Reinwardtia  13(2:  107–123. This  paper  presents  the  results  of  a  two–day  survey  of  the  bryophyte  flora of Mt.  Patuha  and  its  surroundings  near Bandung, West Java, carried out in the framework of the 5th regional training course on bryophyte and lichen diversity and conservation organized by SEAMEO BIOTROP, Bogor, in July 2009. A total of 159 bryophyte species were identi-fied,  including 98 mosses, 60  liverworts, and 1 hornwort, representing almost 1/6 of the  total bryophyte flora of Java. Three moss species, Bryohumbertia subcomosa (Dix. J.–P. Frahm, Fissidens gymnogynus Besch. and F. polypodioidesHedw., and one liverwort, Lejeunea pectinella Mizut., are new additions to the Javanese flora. The bryophyte diversity of Mt. Patuha is well representative of the Malesian flora and is rich in uncommon species. However, the relatively poor representation of shade epiphytes and commonness of sun epiphytes and generalists reflect disturbance of the forest by anthropogenic activities. Careful attention should be given  to conservation of  the  remaining natural  forest  in order  to prevent further losses of the rich bryophyte diversity of the area.

  12. West Nile Disease Epidemiology in North-West Africa: Bibliographical Review.

    Science.gov (United States)

    Benjelloun, A; El Harrak, M; Belkadi, B

    2016-12-01

    West Nile fever (WNF) or West Nile disease (WND) is a mosquito-borne viral disease that can affect birds, humans and horses. West Nile virus (WNV) is a member of the genus Flavivirus in the family Flaviviridae. WNV is maintained in a mosquito-bird-mosquito transmission cycle, whereas humans and horses are considered dead-end hosts. In human and horses, symptoms range from unapparent infection to mild febrile illness, meningitis, encephalitis or death. WNV has a wide geographical range that includes portions of Europe, Asia, Africa, Australia (Kunjin virus), and in North, Central and South America. Migratory birds are thought to be primarily responsible for virus dispersal, including reintroduction of WNV from endemic areas into regions that experience sporadic outbreaks (Fields Virology, 2001, Lippincott Williams and Wilkins, Philadelphia, Pennsylvania, USA, 1043-1125). The occurrence of disease in humans and animals along with birds and mosquitoes surveillance for WNV activity demonstrates that the virus range has dramatically expanded including North, Central and South America as well as Europe and countries facing the Mediterranean Basin. WND infection in humans has been reported in Morocco in 1996 (Virologie, 1, 1997, 248), in Tunisia in 2007 (Ann. N. Y. Acad., 951, 2001, 117) (Med. Trop., 61, 2001, 487) and 2003 (Epidémiologie de la fièvre West Nile, 2012, Thèse de doctorat, Université Montpellier II, Sciences et techniques du Langueduc, Montpellier, France), and in Algeria in 1994 (Rev. Sci. Tech., 31, 2012, 829). Outbreaks of equine encephalitis have been also reported in Morocco in 1996 (Bull. OIE, 11, 1996, 867), in 2003 (Emerg. Infect. Dis., 11, 2005, 306) and in 2010 (World Animal Health Information Database. WAHID, 2010). Serological evidence of WNV has been demonstrated in the three countries in many species. The aim of this review was to assess the epidemiological situation of WND in north-west Africa comprising Morocco, Algeria and Tunisia, with

  13. Rural poverty and development in West Malaysia.

    Science.gov (United States)

    Peacock, F

    1981-07-01

    Rural poverty in West Malaysia during the 1957-1970 period is examined. The period covered was 1 of a high rate of growth combined with an increasing inequality of income and worsening poverty. During the 1955-1970 period, a large amount of development funds, manpower, and expertise was directed towards a reduction of West Malaysia's rural poverty. Despite these efforts, rural poverty increased. Over the period under review, the share of income going to the richest 20% of the population increased from 50% to 56%; the share going to the middle 20% of the population remained constant at 20%; the poorest 60% of the population saw their share of income decline from 30% to 24%. The poorest 40% of the population received only 11.6% of income in 1970. They were predominantly rural, with this sector accounting for 87% of all poverty. The 3 development plans of this period set high aggregate growth rates as the primary targets and emphasized productivity and income in the rural sector. Rural development has not been sold short; the total funding figure of $2,209.46 million represents 40% of all development spending between 1956 and 1970. The money funded 3 broad areas of rural development: replanting of smallholder rubber with high-yielding clones; increasing rice production; and opening new land. The strategy has been to concentrate upon raising the yields from existing farmland and expanding into new areas of settlement. The problem of dealing with poverty in West Malaysia was made worse by the rapid rate of population increase. The population increase of 1,657,000 was absorbed into the traditional smallholder sector, very largely in exisitng areas of settlement. Over the same period, the modern sector of agriculture, the rubber estates, reduced their labor force by 30,000 as they moved into the cultivation of oil palm, a crop requiring less labor. Some of the additional agricultural workers were placed on new land under government land-development and resettlement

  14. The seismicity of Marapi volcano, West Sumatra.

    Science.gov (United States)

    D'Auria, L.

    2009-04-01

    Marapi is one of the active volcanoes in West Sumatra. It is a stratovolcano with an edifice that is elongated in the ENE-WSW direction. Its elevation is about 2,900 m a.s.l. The summit area is characterized by a caldera that contains some active craters aligned along the ENE-WSW direction. The Marapi volcano is an attractive region for tourists and hosts many small communities its surrounding areas. The recent history of Mt. Marapi is characterized by explosive activity at the summit craters. No lava flows have passed the rim of the summit caldera in recent times. The last eruption occurred on August 5, 2004, and consisted of moderate explosive activity from the central crater. In 1975 an eruption with magmatic and phreatic explosive phases and mudflows and lahars occurred that caused fatalities in the surrounding areas. Since 1980 other eruptions have occurred at Marapi volcano. Even if the explosive intensities of those eruptions have been small to moderate, in some cases, there were fatalities. A cooperation project started between Italy and Indonesia (COVIN) for the monitoring of volcanoes in West Sumatra. In the context of this project a monitoring centre has been set up at the Bukittinggi Observatory and a seismological monitoring system for Marapi volcano has been realized. This system is based on a broadband seismic network including 4 three-component stations. The data acquired by the broadband network of Marapi volcano are continuous recordings of the seismic signals starting from 19/10/2006. Volcano-Tectonic and Long Period events of Marapi volcano together with regional and teleseismic earthquakes are recorded. Several events of high magnitude located at short distances from the network were also recorded such as on March 6, 2007, when two events of Magnitudes Mw 6.4 and 6.3 were recorded with the epicentres near the Marapi volcano. During the following days, there was a sequence of hundreds of aftershocks. The preliminary analysis of the seismicity of

  15. Cell-mediated and humoral immunity in west syndrome

    Directory of Open Access Journals (Sweden)

    Terezinha C. B. Montelli

    1981-03-01

    Full Text Available The immunological status of five children with West syndrome consequent to previous cerebral lesions was investigated. Three children had West syndrome and two were in transition from West to Lennox-Gastaut syndrome. All of them showed cellular immunological deficiencies in the following tests: sensitization to DNCB, intracutaneous reaction to PHA, inhibition of leucocyte migration, blastic transformation of lymphocytes, T and B lymphocytes in peripheric blood and levels of serum immunoglobulins. These immunological deficiencies, of different degrees of severity, were associated to frequent infections in these children. A possible association between the immunological deficiencies and autoimmunity is discussed.

  16. West Royalty substation fire post mortem

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2009-05-12

    This post mortem discussed a fire that occurred as a result of three 13.8 kV circuit breakers tripping at a substation control building in Prince Edward Island (PEI). Employees of the electric utility were immediately dispatched to the substation. Between 25,000 and 55,000 customers were without power as a result of firefighters tripping the 138 kV supply to the substation in order to disconnect electricity from the building. A 55 MW combustion turbine was black-started by the operator in order to pick up the electrical load from the Charlottetown substation. Power was gradually restored to utility customers in the region over the next day. An additional generator was used to supply electricity to a final few customers in outlying areas. A subsequent investigation determined that a squirrel caused the breaker to trip. However, a fault in the breaker caused the outage to become more widespread. The utility will continue to investigate methods of improving pest control for substation buildings. A new 13.8 kV circuit will be built to interconnect the Charlottetown and West Royalty substations. 1 fig.

  17. Passive solar systems performance in West Germany

    Energy Technology Data Exchange (ETDEWEB)

    Habenicht, G.

    1986-01-01

    This paper discusses the influence of the two main climatic features (solar radiation and temperature) on the performance of passive solar systems in West Germany. Evaluations were made for the four climatic zones - German Lowlands, German Highlands, Alp Foreland, Alps. These zones differ in solar radiation, temperature and diurnal temperature swings. To evaluate different passive solar systems (direct gain, Trombe wall, water wall) a prototypical dwelling was designed which responded to the environmental conditions. The calculations of the ''Solar Savings Fraction'' (SSF) of each systems were made with a program based on the 'LCR-method'. The paper concludes that although the heating requirements decrease with decreasing latitude and altitude, the SSF is nearly the same for all parts of Germany. They are in a range of +- 5%. This is due to the fact that two main climatic features solar radiation and temperature balance each other. In northern Germany the man solar radiation level is low and so is the number of heating degree days. The conditions in southern Germany are reverse.

  18. GENDERED SPACE IN WEST SUMBA TRADITIONAL HOUSES

    Directory of Open Access Journals (Sweden)

    Esti Asih NURDIAH

    2015-12-01

    Full Text Available Rendell stated that gender representation underlined the production of space in architecture both symbolically and functionally in certain cultures (Rendell et al. 2000. Thus, an exploration on the spatial functionality of traditional houses could show how cultural gender rules and roles generate the spatial arrangements. This empirical research explored the traditional houses in two kampongs: Tarung and Ratenggaro of West Sumba, Indonesia, which spaces are divided into two distinct spaces: male’s space and female’s space, each with its own entrance. This firm division leads to the questions on its relation with the traditional gender roles are represented inside the house. Interestingly, the spatial arrangement is not intended to create separation between men and women inside the house or to pose that the status and roles of men are higher than those of women. The research found that the space separation actually is a manifestation of the dynamic roles of male and female members of the house and the circular arrangement of the space around the fireplace at the centre of the house follows the dynamic of gender duality in Sumba culture.

  19. Happy Environments: Bhutan, Interdependence and the West

    Directory of Open Access Journals (Sweden)

    Randy Schroeder

    2014-05-01

    Full Text Available There is a growing trend to understand economic and environmental policies in terms of multiple dimensions and “interdependence.” Bhutan is increasingly seen as an operational model with its Gross National Happiness (GNH strategy. GNH, which is rooted in Mahayana Buddhism, is a framework and set of policy tools that conceptualizes sustainability as interdependent ecological, economic, social, cultural and good governance concerns. Bhutan’s practical GNH experience illustrates a significant ability to positively couple economic growth with a healthy environment. Can the “West”—with its legacy of either/or economics—learn anything from Bhutan’s multidimensional policy experiment? At first, it would seem not. It is questionable whether the West can replicate Bhutan’s unorthodox policy tools as we do not have a balancing set of Buddhist values rooted in mainstream culture. We are not equipped to respond to the many unintended consequences of interdependent policy because we do not yet understand what “interdependence” actually entails. There is hope, but much of it exists in the grey literature of ecological economics. This literature is in urgent need of greater exposure if we are to imagine and enact sustainability policy tools that are truly sensitive to interdependence, and thus follow Bhutan on its perilous but necessary journey.

  20. Deglacial temperature history of West Antarctica

    Science.gov (United States)

    Cuffey, Kurt M.; Clow, Gary D.; Steig, Eric J.; Buizert, Christo; Fudge, T. J.; Koutnik, Michelle; Waddington, Edwin D.; Alley, Richard B.; Severinghaus, Jeffrey P.

    2016-12-01

    The most recent glacial to interglacial transition constitutes a remarkable natural experiment for learning how Earth’s climate responds to various forcings, including a rise in atmospheric CO2. This transition has left a direct thermal remnant in the polar ice sheets, where the exceptional purity and continual accumulation of ice permit analyses not possible in other settings. For Antarctica, the deglacial warming has previously been constrained only by the water isotopic composition in ice cores, without an absolute thermometric assessment of the isotopes’ sensitivity to temperature. To overcome this limitation, we measured temperatures in a deep borehole and analyzed them together with ice-core data to reconstruct the surface temperature history of West Antarctica. The deglacial warming was 11.3±1.8∘11.3±1.8∘C, approximately two to three times the global average, in agreement with theoretical expectations for Antarctic amplification of planetary temperature changes. Consistent with evidence from glacier retreat in Southern Hemisphere mountain ranges, the Antarctic warming was mostly completed by 15 kyBP, several millennia earlier than in the Northern Hemisphere. These results constrain the role of variable oceanic heat transport between hemispheres during deglaciation and quantitatively bound the direct influence of global climate forcings on Antarctic temperature. Although climate models perform well on average in this context, some recent syntheses of deglacial climate history have underestimated Antarctic warming and the models with lowest sensitivity can be discounted.

  1. Carbon production in comet West 1975n

    Energy Technology Data Exchange (ETDEWEB)

    Feldman, P.D.; Brune, W.H.

    1976-10-01

    Ultraviolet spectra of comet West at moderate resolution in the wavelength range 1200--3200 A were obtained with scanning spectrophotometers aboard an Aerobee 200 rocket launched 1976 March 5.49. The principal emission features observed are lines of C, O, and C/sup +/ and bands of OH, CO, CO/sup +/, and CO/sub 2//sup +/. Estimates of the production rates of OH (and thus H/sub 2/O), C, CO, and O are 9.6, 3.1, 4.2, and 11 (all in units of 10/sup 29/ s/sup -1/), respectively. These rates are consistent with photodissociation of H/sub 2/O and CO from a source in which the CO is one-third as abundant as water. The C I (/sup 1/D--/sup 1/P/sup 0/) line at 1931 A indicates that a large fraction of the carbon is produced in the metastable /sup 1/D state. While CO/sub 2/ cannot be definitively excluded, the spectroscopic evidence favors CO as the major carbon constituent of the comet. (AIP)

  2. William Wilde in the West of Ireland.

    Science.gov (United States)

    Coakley, D

    2016-05-01

    It is widely believed that Sir William Wilde's forebears were in Ireland for just two or three generations. This belief stems from a number of short biographies of Wilde which were published during his lifetime. These biographies gave different versions of the origin of the Wilde family and appear to have been generated by the creative imagination of Lady Jane Wilde or, as she was better known by her nom de plume, Speranza. She was equally imaginative in creating narratives about her own family background and in one she claimed descent from the Italian poet Dante Alighieri. So it was not a great challenge for her to invent biographies of her husband which she deemed suitable for a knight living at the prestigious address of 1 Merrion Square, leading many to believe that William and his son Oscar were more English than Irish. It was also important for Speranza to distance Sir William from any connection which the Wilde family might have had with trade. In this paper published and unpublished material are used, together with a careful examination of family deeds in the Registry of Deeds office, to elucidate the real roots of the Wilde family in Dublin and in the West of Ireland.

  3. Attenuation tomography in West Bohemia/Vogtland

    Science.gov (United States)

    Mousavi, Sima; Haberland, Christian; Bauer, Klaus; Hejrani, Babak; Korn, Michael

    2017-01-01

    We present a three-dimensional (3-D) P-wave attenuation (Qp) model for the geodynamically active swarm earthquake area of West Bohemia/Vogtland in the Czech/German border region. Path-averaged attenuation t* is calculated from amplitude spectra of time windows around the P-wave arrivals of local earthquakes. Average t/t* value or Qp for stations close to Nový Kostel are very low (focal zone (increases up to 500 within 80 km distance). The SIMUL2000 tomography scheme is used to invert the t* for P-wave attenuation perturbation. Analysis of resolution shows that our model is well-resolved in the vicinity of earthquake swarm hypocenters. The prominent features of the model are located around Nový Kostel focal zone and its northern vicinity. Beneath Nový Kostel a vertically stretched (down to depth of 11 km) and a highly attenuating body is observed. We believe that this is due to fracturing and high density of cracks inside the weak earthquake swarm zone in conjunction with presence of free gas/fluid. Further north of Nový Kostel two highly attenuating bodies are imaged which could represent fluid channels toward the surface. The eastern anomaly shows a good correlation with the fluid accumulation area which was suggested in 9HR seismic profile.

  4. Petrojarl Foinaven -- A West of Shetland challenge

    Energy Technology Data Exchange (ETDEWEB)

    Boerresen, R.; Krafft, H.

    1996-12-31

    The Foinaven field was first discovered in late 1992. The field will produce oil for the partners BP and Shell in 1996. The owners laid out a commercial strategy in line with the CRINE initiative for the field development. The Field will be produced by a Production Ship, hooked up to the subsea production system via flexible risers and umbilicals. The 300,000 barrels storage capacity ship, Petrojarl Foinaven, will be turret moored in the field. The technical challenges in the Production Ship design have been closely related to the field location in the Atlantic Ocean west of Shetlands. Here the water depth is 500 meters, and the weather is blessed with a climate harsher than the North Sea (e.g., the 100 year wave is some 18 meter significant). The ship solution has been designed by Golar-Nor Offshore, and is being built by Astano shipyard, with a McDermott Engineering oil process design. The ship is owned and operated by Golar-Nor Offshore under a lease agreement with the field operator BP. This presentation will address aspects of the Production Ship design, being one element at the Foinaven Field development. The other element is the subsea system described in another paper in this conference.

  5. ETHNO SEPARATIST TERRORISM IN WEST EUROPE

    Directory of Open Access Journals (Sweden)

    Pashchenko L. V.

    2016-04-01

    Full Text Available For the purpose of this article, the analysis of the development of ethnoseparatism in West Europe has been undertaken. Under current conditions, the ethnical separatism threatens national security of series of European countries and territorial integrity. Special attention has been paid to social and political beginnings and causes of terrorism in Spain and Great Britain. The activity of such terrorist organizations as IRA and ETA has been researched. Ideological basis of violence, purposes and objectives, as well as the main stages of formation and development of these organizations have been disclosed. The author states that the use of the terrorism as a method of political struggle is destructive and does not facilitate a solution to the national problems. We have also considered approaches of the governments to solve the problems of separatism and terrorism on the territory of these countries. In their counter-terrorism efforts political leaders of Spain and Great Britain have used both methods of force and political and legal methods, namely, granting to the regions of wide economical and political independence, preservation of authentic cultural values and language of indigenous people. The formation of violence intolerance in public conscience as control method has become the main direction of the antiterrorist policy in the countries under consideration. Anti-terrorist model which has been developed by the European countries can be effective and useful for the regions being in ethnopolitical conflicts

  6. [West Nile virus: a reality in Mexico].

    Science.gov (United States)

    Téllez, Ildefonso; Calderón, Oscar; Franco-Paredes, Carlos; del Río, Carlos

    2006-01-01

    West Nile virus (WNV) is a RNA virus of the Flaviridae, genus flavivirus family. It is a neuropathogenic virus causing disease in birds, horses and humans. WNVis transmitted by the vector mosquito Culex sp. The virus life 's cycle includes mosquitoes as vectors and birds as natural hosts. Humans are accidental hosts. Since the introduction of the Epidemiological Surveillance Program at the Ministry ofHealth. we have documented 90 positive test results among birds out of 1,223 cases studied in Mexico as of September IS. 2005. The incubation period in humans after a mosquito bite ranges from 3 to 14 days. Disease is characterized by early onset fever, general malaise, decreased appetite, nausea, vomiting, headaches, myalgias, enlarged lymph nodes andrash. Neurological manifestations include encephalitis andflaccid paralysis, which are present in less than 1% of subjects infected with WNV. Older patients display more adverse outcomes including death. The diagnosis is made by the determination of specific IgM and JgG antibodies in serum and/or cerebrospinal fluid. There is no antiviral treatment to date against WNV but interferon ?2b, and WNVspec4ic-immunoglobulin have been used Prevention is therefore the key to control the infection.

  7. ESSEA On-Line Courses and the WestEd Eisenhower Regional Consortium (WERC)

    Science.gov (United States)

    Rognier, E.

    2001-12-01

    The WestEd Eisenhower Regional Consortium (WERC) is in its second year of offering two Earth Systems Science On-line Graduate courses from IGES - one for High School teachers, and one for Middle School teachers. These high-quality courses support WERC's commitment to "supporting increased scientific and mathematical literacy among our nation's youth through services and other support aimed at enhancing the efforts of those who provide K-12 science and mathematics education." WERC has been able to use its EdGateway online community network to offer these courses to environmental education and science teachers nationwide. Through partnerships with the North American Association for Environmental Education (NAAEE), the National Environmental Education Advancement Project (NEEAP), and other regional, state and local science and environmental education organizations, WERC has a broad reach in connecting with science educators nationwide. WERC manages several state and national listservs, which enable us to reach thousands of educators with information about the courses. EdGateway also provides a private online community in which we offer the courses. WERC partners with two Master Teachers from Utah, who facilitate the courses, and with the Center for Science and Mathematics Education at Weber State University, who provides low-cost graduate credit for the courses. Our students have included classroom teachers from upper elementary through high school, community college science teachers, and environmental science center staff who provide inservice for teachers. Educators from Hawaii to New Jersey have provided diverse personal experiences of Earth Systems Science events, and add richness to the online discussions. Two Earth Science Experts, Dr. Rick Ford from Weber State University, and Dr. Art Sussman from WestEd also contribute to the high caliber of learning the students experience in the courses. (Dr. Sussman's book, Dr. Art's Guide to Planet Earth, is used as one of

  8. West Coast Observing System (WCOS) ADCP Currents Data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The West Coast Observing System (WCOS) project provides access to temperature and currents data collected at four of the five National Marine Sanctuary sites,...

  9. Digital elevation model of Walker Lake, West-Central Nevada

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Walker Lake lies within a topographically closed basin in west-central Nevada and is the terminus of the Walker River. Accurately determining the bathymetry and...

  10. Unravelling institutional determinants affecting change in agriculture in West Africa

    NARCIS (Netherlands)

    Struik, P.C.; Klerkx, L.W.A.; Hounkonnou, D.

    2014-01-01

    This paper compares lessons learned from nine studies that explored institutional determinants of innovation towards sustainable intensification of West African agriculture. The studies investigated issues relating to crop, animal, and resources management in Benin, Ghana, and Mali. The constraints

  11. Physico- Chemical characteristics of compost (Cotonou, Benin, West ...

    African Journals Online (AJOL)

    Physico- Chemical characteristics of compost (Cotonou, Benin, West Africa) ... proving the poverty of the soil of this site and their increased need of organic amendment. Keywords: Cotonou; biodegradable waste; compost; physico- chemical ...

  12. West Coast Observing System (WCOS) ADCP Station Data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The West Coast Observing System (WCOS) project provides access to temperature and currents data collected at four of the five National Marine Sanctuary sites,...

  13. West Knox Pond water budget and water quality

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The purpose of this report is to analyze the water budget and water quality for West Knox Pond for the May through September period of 2002 and 2003. The...

  14. Herbicide concentrations in wetlands in west central Minnesota, 1992

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Thirty emergent, seasonally to semi-permanently flooded wetlands in an intensively farmed area of west central Minnesota were sampled before and during the 1992 crop...

  15. VT West Branch Natural Channel Post Monitoring 2003

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) Because of the well known problems of the river, the West Branch was identified as a restoration implementation site to be funded as part of the...

  16. Soil transmitted helminth infection among farmers in Ukwa West ...

    African Journals Online (AJOL)

    Soil transmitted helminth infection among farmers in Ukwa West Local Government Area, Abia State, south-east, Nigeria. ... Open Access DOWNLOAD FULL TEXT ... Collected data were analysed using descriptive and analytical tools. Results: ...

  17. The Suppression of Internal Unrest in South West Africa (Namibia ...

    African Journals Online (AJOL)

    AM Fokkens

    the authority to rule South West Africa as a mandated territory from 1. January 1921.1 ..... Ryneveld, Director Air Services, was in command of the aeroplanes. The ... The Bondelswarts organised their hierarchy, with Cristiaan as the hereditary ...

  18. Salmonellosis in passerine birds in Maryland and West Virginia

    Science.gov (United States)

    Locke, L.N.; Shillinger, R.B.; Jareed, T.

    1973-01-01

    Salmonella typhimurium was responsible for a die-off of evening grosbeaks (Hesperiphona vespertina) at Elkins, West Virginia, and was isolated from a pine siskin (Spinus pinus) collected at the site of a die-off near Baltimore, Maryland.

  19. K-East and K-West Reactors

    Data.gov (United States)

    Federal Laboratory Consortium — Hanford's "sister reactors", the K-East and the K-West Reactors, were built side-by-side in the early 1950's. The two reactors went operational within four months of...

  20. Port viability for choice making among shipping companies in West ...

    African Journals Online (AJOL)

    Discriminant analysis was used to ascertain the consistency of the attributes of ports that ... that could influence the port viability and improve import capacity building. ... Keywords: Port viability, shipping companies, trade route, West Africa

  1. Littoral transport studies along west coast of India - A reivew

    Digital Repository Service at National Institute of Oceanography (India)

    Kunte, P.D.; Wagle, B.G.; Sugimori, Y.

    This article presents an overview of investigations that were carried out to determine the dynamics of littoral transport along the west coast of India, during last three decades. Sedimentological methods, wave refraction diagrams, tracer tracking...

  2. Longshore sediment transport model for the Indian west coast

    Digital Repository Service at National Institute of Oceanography (India)

    Chandramohan, P.; Nayak, B.U.

    Longshore sediment transport rates for the Indian west coast from Cochin to Porbandar are estimated from ship observed wave data (1968 to 1986). The sediment transport rate is relatively high during the southwest monsoon period from June...

  3. Some Actiniaria (Cnidaria: Anthozoa) from the west coast of India

    Digital Repository Service at National Institute of Oceanography (India)

    Hartog, J. C. Den; Jayasree, V.

    A small collection of five species of Actiniaria from the west coast of India, including three new species, is described and discussed. It concerns Anthopleura anjunae spec. nov., Bunodosoma goanensis spec. nov., Synantheopsis parulekari spec. nov...

  4. Tides in the Gulf of Khambhat, west coast of India

    Digital Repository Service at National Institute of Oceanography (India)

    Nayak, R.K.; Shetye, S.R.

    Semi-diurnal tides in the Gulf of Khambhat (Cambay), west coast of India, amplify about threefold from mouth to head. In contrast, the amplification of diurnal tides is much smaller. A one-dimensional barotropic model with channel geometry...

  5. Key West, Florida Tsunami Forecast Grids for MOST Model

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Key West, Florida Forecast Model Grids provides bathymetric data strictly for tsunami inundation modeling with the Method of Splitting Tsunami (MOST) model. MOST...

  6. Spitbase of west coast of India: Potential appraisal

    Digital Repository Service at National Institute of Oceanography (India)

    Kunte, P.D.; Wagle, B.G.

    Spectacular spits of different sizes and variety of shapes bordering west coast of India were mapped with remote sensing techniques. By gathering information on geomorphological and locational parameters of spits, "SPITBASE", a database of spits...

  7. Characterizing freshwater and nutrient fluxes to West Falmouth Harbor, Massachusetts

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — These data present oceanographic and water-quality observations made at 4 locations in West Falmouth Harbor and 3 in Buzzards Bay, Massachusetts. While both Buzzards...

  8. activities of the ap leventis, the west african foremost ornithological ...

    African Journals Online (AJOL)

    Administrator

    This may be because of their high mobility, which make it easy for ... postgraduate students at Masters level have so far graduated, and are now ... Garden Warbler Sylvia borin migration in sub-Saharan West Africa: Phenology and body mass.

  9. West antarctic ice sheet collapse: Chimera or clear danger

    Energy Technology Data Exchange (ETDEWEB)

    Alley, R.B. (Pennsylvania State Univ., University Park, PA (United States)); MacAyeal, D.R. (Univ. of Chicago, IL (United States))

    1993-01-01

    The specter of a west antarctic collapse has been with us for 25 years. Recently, certain official assessments concerned primarily with the future response to projected global warming have concluded that Antarctica will not cause much sea-level rise within the planning horizon of a century or so. At the same time startling new results on ice sheet (in)stability have been emerging, pointing to less stability then previously believed. Some recent results are reviewed briefly: Heinrich layers in the North Atlantic show basally lubricated surges of the Laurentide ice sheet; the west antarctic ice sheet collapsed recently; the modern west antarctic ice sheet is changing rapidly locally; the bed of ice stream B is exceptionally well lubricated by water and water-saturated soft sediments; the modern ice sheet is thinning slowly on average; a model west antarctic ice sheet undergoes rapid collapses long after forcing and probably related to penetration of warmth to the bed. 23 refs.

  10. The landscapes of West Africa—40 years of change

    Science.gov (United States)

    Cotillon, Suzanne E.

    2017-02-16

    What has driven changes in land use and land cover in West Africa over the past 40 years? What trends or patterns can be discerned in those changes? To answer these questions, the U.S. Geological Survey West Africa Land Use Dynamics project partnered with the Permanent Interstate Committee for Drought Control in the Sahel and the U.S. Agency for International Development/West Africa to map land use and land cover across the region for  three time periods (years): 1975, 2000, and 2013. This cooperative effort has resulted in the publication of a 219-page atlas, “Landscapes of West Africa: A Window on a Changing World.” The atlas uses satellite imagery, maps, and pictures to tell a complex story of landscape change at regional and national scales. It includes a collection of focused studies, some of which raise cause for concern, and others that provide considerable hope.

  11. VT West Branch Natural Channel Design Restoration 2001-2002

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) Because of the well known problems of the river, the West Branch was identified as a restoration implementation site to be funded as part of the...

  12. Resurgent Military Political Adventurism in West Africa: Implications ...

    African Journals Online (AJOL)

    DR Nneka

    military intervention in the politics of some West African states and this calls for concern as ..... the elections have been considered not free and fair. Liberia .... Senegalese President Abdoulaye Wade came to power in 2000 riding an electoral ...

  13. The West Perfecting Its Techniques To Hurt China

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    Spanish Website Rebellion published an article titled The West Perfecting Its Techniques to Hurt China by U.S. writer, filmmaker and journalist Andre Vltchek on November 12, 2010. Excerpts from the article follow:

  14. West African Journal of Pharmacology and Drug Research ...

    African Journals Online (AJOL)

    West African Journal of Pharmacology and Drug Research. ... related topics including chemotherapy and toxicology will be considered for publication. ... Introduction: This should begin on a new page and should contain a brief account of the ...

  15. Markets, Climate Change and Food Security in West Africa

    Science.gov (United States)

    Brown, Molly E.; Hintermann, Beat; Higgins, Nathaniel

    2009-01-01

    West Africa is one of the most food insecure regions of the world. Sharply increased food and energy prices in 2008 brought the role of markets in food access and availability around the world into the spotlight, particularly in urban areas. The period of high prices had the immediate consequence of sharply increasing the number of hungry people in the region without boosting farmer incomes significantly. In this article, the interaction between markets, food prices, agricultural technology and development is explored in the context of West Africa. To improve food security in West Africa, sustained commitment to investment in the agriculture sector will be needed to provide some protection against global swings in both production and world markets. Climate change mitigation programs are likely to force global energy and commodity price increases in the coming decades, putting pressure on regions like West Africa to produce more food locally to ensure stability in food security for the most vulnerable.

  16. 21 CFR 866.3940 - West Nile virus serological reagents.

    Science.gov (United States)

    2010-04-01

    ..., from individuals who have signs and symptoms consistent with viral meningitis/encephalitis. The detection aids in the clinical laboratory diagnosis of viral meningitis/encephalitis caused by West...

  17. The landscapes of West Africa—40 years of change

    Science.gov (United States)

    Cotillon, Suzanne E.

    2017-02-16

    What has driven changes in land use and land cover in West Africa over the past 40 years? What trends or patterns can be discerned in those changes? To answer these questions, the U.S. Geological Survey West Africa Land Use Dynamics project partnered with the Permanent Interstate Committee for Drought Control in the Sahel and the U.S. Agency for International Development/West Africa to map land use and land cover across the region for  three time periods (years): 1975, 2000, and 2013. This cooperative effort has resulted in the publication of a 219-page atlas, “Landscapes of West Africa: A Window on a Changing World.” The atlas uses satellite imagery, maps, and pictures to tell a complex story of landscape change at regional and national scales. It includes a collection of focused studies, some of which raise cause for concern, and others that provide considerable hope.

  18. The Limestone Glades And Barrens Of West Virginia

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Cedar glade, limestone barren, and glade woodland communities are reported from limestone terranes in the Ridge and Valley of northeastern West Virginia. The cedar...

  19. West African Journal of Industrial and Academic Research: Editorial ...

    African Journals Online (AJOL)

    West African Journal of Industrial and Academic Research: Editorial Policies ... computers, mathematics, control and information technology, physics, industrial electronics, management sciences and engineering, industry chemistry, general technology rocketry, space and astronomy, earth sciences, ... Peer Review Process.

  20. Zonation of uplifted pleistocene coral reefs on barbados, west indies.

    Science.gov (United States)

    Mesolella, K J

    1967-05-05

    The coral species composition of uplifted Pleistocene reefs on Barbados is very similar to Recent West Indian reefs. Acropora palmata, Acropora cervicornis, and Montastrea annularis are qtuantitatively the most important of the coral species.

  1. Climate Prediction Center (CPC) West Pacific Teleconnection Pattern Index

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Monthly tabulated index of the West Pacific (WP) teleconnection pattern. The data spans the period 1950 to present. The index is derived from a rotated principal...

  2. NNDSS - Table II. Varicella to West Nile virus disease

    Data.gov (United States)

    U.S. Department of Health & Human Services — NNDSS - Table II. Varicella to West Nile virus disease - 2014. In this Table, all conditions with a 5-year average annual national total of more than or equals 1,000...

  3. Argus Energy WV, LLC wins 2007 Wetlands West Virginia Award

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-15

    Argus Energy's Kiah Creek Operation has received the 2007 Wetlands West Virginia Award presented by the West Virginian Coal Association. The operation was originally a 1267 acre underground mine in the Coalburg seam. Underground mining commenced in 2000 until the end of 2003 with more than two million tons of coal being produced. The creation of the wetlands was achieved during the operations. 8 photos.

  4. Imported West Nile virus encephalitis in an Israeli tourist.

    Science.gov (United States)

    Rogers, Benjamin A; Hueston, Linda; Ratnam, Irani

    2009-08-17

    West Nile virus is an arbovirus that has caused large outbreaks of febrile illness, meningitis and encephalitis in Europe, North America and the Middle East. We describe the first laboratory-confirmed human case of West Nile virus infection in Australia, in a 58-year-old tourist who was almost certainly infected in Israel. The case is a reminder of the need to consider exotic pathogens in travellers and of the risk of introducing new pathogens into Australia.

  5. 昆明地区5岁以下儿童博卡病毒和偏肺病毒感染的临床流行病学调查%Clinical Epidemiology of Human Bocavirus and Metapneumovirus Infection among Children under 5 years in Kunming

    Institute of Scientific and Technical Information of China (English)

    李秀云; 孔艳; 马克玲; 郑文静; 尚晓丽; 符宗敏; 夏晓玲

    2012-01-01

    Objective To investigate the prevalence, epidemiology and clinical features of Human Bocavirus (HBoV) and Human Metapneumovirus (hMPV) infection in children with respiratory tract infection in Kunming. Methods From October 2009 to December 2010, 550 nasal swab samples were collected from children under 5 years with respiratory tract infection. Multiplex polymerase chain reaction was used to detect 21 common pathogens including HBoV and hMPV. At the same time, the baseline characteristics of children and clinical manifestation were recorded by researchers through structure questionnaire. SPSS software was used to analyze data. Results Out of 550 nasopharyngeal swab specimens, 18 specimens were detected with HBoV infection and 29 samples were detected with hMPV infection. The positive rate of HBoV and hMPV was 3.3% and 5.3%, respectively. Among 18 HBoV positive samples, there were 17 samples mixed other pathogen infection. The mixed infection rate of HBoV accounted for 94.4%. Meanwhile, among 29 hMPV positive specimens, the co-infection rate was 58.6%. The most common pathogens of mixed infection with HBoV and hMPV were respiratory syncytial virus, rhinovirus, influenzaA virus and Haemophilus influenza. Infant got higher HBoV infection. There was no significant difference between different age groups of hMPV infection. The peak of positive infection rate of HBoV and hMPV were both in autumn and winter. Fever, cough and wheezing were the major clinical manifestation of children with HBoV and hMPV infection. Conclusions HBoV and hMPV are important pathogens of acute respiratory tract infection in children in Kunming. The prevalent seasons are autumn and winter for the two virus infection.%目的 了解昆明地区5岁以下急性呼吸道感染患儿中人类博卡病毒(HBoV)和人类偏肺病毒(hMPV)的流行状况、混合感染情况以及主要的临床表现特点.方法 按照病例纳入标准,采集2009年9月至2010年9月在昆明地区6所不同区域医

  6. The 1983 drought in the West Sahel: a case study

    Energy Technology Data Exchange (ETDEWEB)

    Bader, Juergen [Bjerknes Centre for Climate Research, Bergen (Norway); University of Bergen, Geophysical Institute, Bergen (Norway); Latif, Mojib [Leibniz-Institute for Marine Sciences at the University of Kiel, Kiel (Germany)

    2011-02-15

    Some drought years over sub-Saharan west Africa (1972, 1977, 1984) have been previously related to a cross-equatorial Atlantic gradient pattern with anomalously warm sea surface temperatures (SSTs) south of 10 N and anomalously cold SSTs north of 10 N. This SST dipole-like pattern was not characteristic of 1983, the third driest summer of the twentieth century in the Sahel. This study presents evidence that the dry conditions that persisted over the west Sahel in 1983 were mainly forced by high Indian Ocean SSTs that were probably remanent from the strong 1982/1983 El Nino event. The synchronous Pacific impact of the 1982/1983 El Nino event on west African rainfall was however, quite weak. Prior studies have mainly suggested that the Indian Ocean SSTs impact the decadal-scale rainfall variability over the west Sahel. This study demonstrates that the Indian Ocean also significantly affects inter-annual rainfall variability over the west Sahel and that it was the main forcing for the drought over the west Sahel in 1983. (orig.)

  7. A Regional Approach to Market Monitoring in the West

    Energy Technology Data Exchange (ETDEWEB)

    Barmack, Matthew; Kahn, Edward; Tierney, Susan; Goldman, Charles

    2006-10-01

    Market monitoring involves the systematic analysis of pricesand behavior in wholesale power markets to determine when and whetherpotentially anti-competitive behavior is occurring. Regional TransmissionOrganizations (RTOs) typically have a market monitoring function. Becausethe West does not have active RTOs outside of California, it does nothave the market monitoring that RTOs have. In addition, because the Westoutside of California does not have RTOs that perform centralized unitcommitment and dispatch, the rich data that are typically available tomarket monitors in RTO markets are not available in the West outside ofCalifornia. This paper examines the feasibility of market monitoring inthe West outside of California given readily available data. We developsimple econometric models of wholesale power prices in the West thatmight be used for market monitoring. In addition, we examine whetherproduction cost simulations that have been developed for long-runplanning might be useful for market monitoring. We find that simpleeconometric models go a long ways towards explaining wholesale powerprices in the West and might be used to identify potentially anomalousprices. In contrast, we find that the simulated prices from a specificset of production cost simulations exhibit characteristics that aresufficiently different from observed prices that we question theirusefulness for explaining price formation in the West and hence theirusefulness as a market monitoring tool.

  8. Sequestration Options for the West Coast States

    Energy Technology Data Exchange (ETDEWEB)

    Myer, Larry

    2006-04-30

    The West Coast Regional Carbon Sequestration Partnership (WESTCARB) is one of seven partnerships that have been established by the U.S. Department of Energy (DOE) to evaluate carbon capture and sequestration (CCS) technologies best suited for different regions of the country. The West Coast Region comprises Arizona, California, Nevada, Oregon, Washington, Alaska, and British Columbia. Led by the California Energy Commission, WESTCARB is a consortium of about 70 organizations, including state natural resource and environmental protection agencies; national laboratories and universities; private companies working on carbon dioxide (CO{sub 2}) capture, transportation, and storage technologies; utilities; oil and gas companies; nonprofit organizations; and policy/governance coordinating organizations. Both terrestrial and geologic sequestration options were evaluated in the Region during the 18-month Phase I project. A centralized Geographic Information System (GIS) database of stationary source, geologic and terrestrial sink data was developed. The GIS layer of source locations was attributed with CO{sub 2} emissions and other data and a spreadsheet was developed to estimate capture costs for the sources in the region. Phase I characterization of regional geological sinks shows that geologic storage opportunities exist in the WESTCARB region in each of the major technology areas: saline formations, oil and gas reservoirs, and coal beds. California offers outstanding sequestration opportunities because of its large capacity and the potential of value-added benefits from enhanced oil recovery (EOR) and enhanced gas recovery. The estimate for storage capacity of saline formations in the ten largest basins in California ranges from about 150 to about 500 Gt of CO{sub 2}, the potential CO{sub 2}-EOR storage was estimated to be 3.4 Gt, and the cumulative production from gas reservoirs suggests a CO{sub 2} storage capacity of 1.7 Gt. A GIS-based method for source

  9. West Coast Groundfish Bottom Trawl Survey Data - Annual West Coast time series groundfish trawl data collection survey

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Members of the Fishery Resource Analysis and Monitoring Division Survey Team conduct an annual West Coast Groundfish Bottom Trawl Survey from May - October each...

  10. Neuromuscular Manifestations of West Nile Virus Infection

    Directory of Open Access Journals (Sweden)

    A. Arturo eLeis

    2012-03-01

    Full Text Available The most common neuromuscular manifestation of West Nile virus (WNV infection is a poliomyelitis syndrome with asymmetric paralysis variably involving one (monoparesis to four limbs (quadriparesis, with or without brainstem involvement and respiratory failure. This syndrome of acute flaccid paralysis may occur without overt fever or meningoencephalitis. Although involvement of anterior horn cells in the spinal cord and motor neurons in the brainstem are the major sites of pathology responsible for neuromuscular signs, inflammation also may involve skeletal or cardiac muscle (myositis, myocarditis, motor axons (polyradiculitis, peripheral nerve (Guillain-Barré syndrome, brachial plexopathy. In addition, involvement of spinal sympathetic neurons and ganglia provides a plausible explanation for autonomic instability seen in some patients. Many patients also experience prolonged subjective generalized weakness and disabling fatigue. Despite recent evidence that WNV may persist long term in the central nervous system or periphery in animals, the evidence in humans is controversial. WNV persistence would be of great concern in immunosuppressed patients or in those with prolonged or recurrent symptoms. Support for the contention that WNV can lead to autoimmune disease arises from reports of patients presenting with various neuromuscular diseases that presumably involve autoimmune mechanisms (GBS, other demyelinating neu¬ropathies, myasthenia gravis, brachial plexopathies, stiff-person syndrome, and delayed or recurrent symptoms. Although there is no specific treatment or vaccine currently approved in humans, and the standard remains supportive care, drugs that can alter the cascade of immunobiochemical events leading to neuronal death may be potentially useful (high-dose corticosteroids, interferon preparations, and intravenous immune globulin containing WNV-specific antibodies. Human experience with these agents seems promising based on anecdotal

  11. Contaminants in two West Greenland caribou populations

    Energy Technology Data Exchange (ETDEWEB)

    Gamberg, Mary, E-mail: mary.gamberg@gmail.com [Gamberg Consulting, 708 Jarvis St., Whitehorse, Yukon Y1A 2J2 (Canada); Cuyler, Christine [Greenland Institute of Natural Resources, PO Box 570, 3900 Nuuk, Greenland (Denmark); Wang, Xiaowa [Environment Canada, 867 Lakeshore Rd., Burlington, ON L7S 1A1 (Canada)

    2016-06-01

    Two caribou populations in West Greenland were sampled and the kidneys, liver and muscle analyzed for contaminants, including aluminum, arsenic, cadmium, copper, lead, mercury, selenium and zinc. Although close in proximity, the two populations are topographically separated by an ice cap, which creates different climates and vegetation types in each region. Contaminant levels reflected the differing diets of the two caribou populations. To the south in the wetter lichen-rich region, caribou had significantly more aluminum, arsenic, cadmium, lead, mercury, selenium and zinc, likely due to atmospheric deposition on lichens. To the north in the dry desert steppe where grasses predominate, caribou had higher levels of copper. Cows collected in late winter had significantly less hepatic copper, lead and mercury if pregnant, indicating placental transfer of these elements. Our results suggest that hepatic copper levels < 200 μg g{sup −1} dry weight may result in copper depletion in pregnant cows and hepatic mercury concentrations above 0.5 μg g{sup −1} dry weight may negatively affect fertility in caribou cows. Hepatic mercury levels were negatively correlated with cow body weight, suggesting an adverse effect on body condition. Element concentrations found in tissues from these caribou are not considered to be of a health concern for those consuming this traditional food. - Highlights: • Caribou tissue contaminant profiles may reflect different diets. • Low hepatic copper may result in copper depletion in pregnant caribou. • High hepatic mercury may negatively affect fertility in caribou cows. • Hepatic mercury is negatively correlated with body condition in caribou cows. • Metal levels in tissues are not a health concern to people consuming caribou.

  12. Analysis of K west basin canister gas

    Energy Technology Data Exchange (ETDEWEB)

    Trimble, D.J., Fluor Daniel Hanford

    1997-03-06

    Gas and Liquid samples have been collected from a selection of the approximately 3,820 spent fuel storage canisters in the K West Basin. The samples were taken to characterize the contents of the gas and water in the canisters providing source term information for two subprojects of the Spent Nuclear Fuel Project (SNFP) (Fulton 1994): the K Basins Integrated Water Treatment System Subproject (Ball 1996) and the K Basins Fuel Retrieval System Subproject (Waymire 1996). The barrels of ten canisters were sampled for gas and liquid in 1995, and 50 canisters were sampled in a second campaign in 1996. The analysis results from the first campaign have been reported (Trimble 1995a, 1995b, 1996a, 1996b). The analysis results from the second campaign liquid samples have been documented (Trimble and Welsh 1997; Trimble 1997). This report documents the results for the gas samples from the second campaign and evaluates all gas data in terms of expected releases when opening the canisters for SNFP activities. The fuel storage canisters consist of two closed and sealed barrels, each with a gas trap. The barrels are attached at a trunion to make a canister, but are otherwise independent (Figure 1). Each barrel contains up to seven N Reactor fuel element assemblies. A gas space of nitrogen was established in the top 2.2 to 2.5 inches (5.6 to 6.4 cm) of each barrel. Many of the fuel elements were damaged allowing the metallic uranium fuel to be corroded by the canister water. The corrosion releases fission products and generates hydrogen gas. The released gas mixes with the gas-space gas and excess gas passes through the gas trap into the basin water. The canister design does not allow canister water to be exchanged with basin water.

  13. Excerpt from East Is West and West Is East: Gender, Culture, and Interwar Encounters between Asia and America

    OpenAIRE

    Karen Kuo

    2013-01-01

    Excerpted from Karen Kuo, East Is West and West Is East: Gender, Culture, and Interwar Encounters between Asia and America (Philadelphia: Temple University Press, 2012).Reprinted with permission from Temple University Press.

  14. Up-to-date knowledge of West Nile virus infection

    Directory of Open Access Journals (Sweden)

    Hrnjaković-Cvjetković Ivana

    2009-01-01

    Full Text Available Virus West Nile virus is a single-stranded RNA virus of the family Flaviviridae, genus Flavivirus. Epidemiology West Nile virus is maintained in the cycle involving culicine mosquitoes and birds .Humans typically acquire West Nile infection through a bite from infected adult mosquito. Person to person transmission can occur through organ transplantation, blood and blood product transfusions, transplacentally and via brest milk. Human cases of West Nile infections were recorded in Africa, Israel, Russia, India, Pakistan. In Romania in 1996 West Nile fever occurred with hundreds of neurologic cases and 17 fatalities. First human cases in the United States were in New York City where 59 persons were infected and had fever, meningitis, encephalitis and flaccid paralysis. Clinical manifestation Most human cases are asymptomatic. The majority of symptomatic patients have a self limited febrile illness. Fatigue, nausea, vomiting, eye pain, headache, myalgias, artralgias, lymphadenopathy and rash are common complaints. Less than 1% of all infected persons develop more severe neurologic illness including meningitis, encefalitis and flaccid paralysis. Laboratory diagnosis Diagnosis of West Nile virus infection is based on serologic testing, isolation of virus from patient samples and detection of viral antigen or viral genom. ELISA test and indirect immunofluorescenceassay are used for detecting IgM and IgG antibodies in serum and cerebrospinal fluid. Treatment In vitro studies have suggested that ribavirin and interferon alfa -2b may be useful in the treatment of West Nile virus disease. Prevention The most important measures are mosquito control program and personal protective measures. .

  15. Islam, Islamophobia and the West: Opposing Views and Social Reality

    Directory of Open Access Journals (Sweden)

    Adila Pavelić

    2015-12-01

    Full Text Available Through reviewing different and often opposing views on the relationship between Islam and the West, the paper aims to offer a better understanding of this relationship, and of Islamophobia as an old, yet increasingly actual and in many respects relevant social problem. A short historical overview of the understanding of the relationship between Islam and the West, and various definitions of Islamophobia, are followed by examination of some recent works that from different perspectives deal with the issue. Firstly, is given insight into the works written by Western theorists who describe the relationship between Islam and the West within the framework of the idea of the clash of civilizations while, at the same time, labeling Islam often as a non-modern religion and culture whose members reject Western values such as democracy, freedom and gender equality. In contrast to them are introduced the authors whose argumentation opposes stereotypical views of Islam; among them are also those that critically address Islamophobia in today’s Western society, either by presenting results of their research on Islamophobia or by documenting the dominant media image of Islam, which has been perpetuating stereotypes, especially in the aftermath of the terrorist attacks on New York in 2001. Lastly, it is concluded that, regardless of the origin of works dealing with the relationship between Islam and the West or of the type of their arguments, they more often than not interpret Islam and the West primarily as two separate, self-contained and in itself incompatible worlds. In doing so, the authors here argue, they employ an essentialist and reductionist approach not only to Islam – as it might be assumed – but also to the West, while the relationship between Islam and the West is simplified and schematized.

  16. Serologic survey of West Nile virus in horses from Central-West, Northeast and Southeast Brazil

    Science.gov (United States)

    Silva, Jaqueline Raymondi; de Medeiros, Larissa Campos; dos Reis, Vinícius Pinho; Chávez, Juliana Helena; Munhoz, Thiago Demarchi; Borges, Gustavo Puia; Soares, Otavio Augusto Brioschi; de Campos, Carlos Henrique Coelho; Machado, Rosângela Zacarias; Baldani, Cristiane Divan; Silva, Maria Luana Cristiny Rodrigues; Faria, Joice Lara Maia; da Silva, Edson Elias; Figueiredo, Luiz Tadeu Moraes

    2013-01-01

    Since the emergence of West Nile virus (WNV) in North America in 1999, there have been several reports of WNV activity in Central and South American countries. To detect WNV in Brazil, we performed a serological survey of horses from different regions of Brazil using recombinant peptides from domain III of WNV. Positive samples were validated with the neutralisation test. Our results showed that of 79 ELISA-positive horses, nine expressed WNV-specific neutralising antibodies. Eight of the infected horses were from the state of Mato Grosso do Sul and one was from the state of Paraíba. Our results provide additional evidence for the emergence of WNV in Brazil and for its circulation in multiple regions of the country. PMID:24037110

  17. Serologic survey of West Nile virus in horses from Central-West, Northeast and Southeast Brazil

    Directory of Open Access Journals (Sweden)

    Jaqueline Raymondi Silva

    2013-11-01

    Full Text Available Since the emergence of West Nile virus (WNV in North America in 1999, there have been several reports of WNV activity in Central and South American countries. To detect WNV in Brazil, we performed a serological survey of horses from different regions of Brazil using recombinant peptides from domain III of WNV. Positive samples were validated with the neutralisation test. Our results showed that of 79 ELISA-positive horses, nine expressed WNV-specific neutralising antibodies. Eight of the infected horses were from the state of Mato Grosso do Sul and one was from the state of Paraíba. Our results provide additional evidence for the emergence of WNV in Brazil and for its circulation in multiple regions of the country.

  18. Completion of electrification between Aachen West and Montzen; Durchgehende Elektrifizierung Aachen West-Montzen

    Energy Technology Data Exchange (ETDEWEB)

    Hinzen, A. [DB Netz AG, Frankfurt am Main (Germany). Internationale Infrastrukturangelegenheiten

    2007-05-15

    The railway line between Aachen West and Montzen (and from there on to Antwerp) is one of the most important long-distance freight lines in Europe and today carries all the rail freight moved directly between Germany and Belgium. Despite that, a part of it has still not been electrified. The line was built during the First World War, when the territory was under German occupation. The two railway infrastructure managers concerned, Infrabel (Belgium) and DB Netz (Germany), are working on plans to plug this gap in the electrification of the European freight network ready for the timetable change in December 2008. This article, penned by a specialist, describes the vicissitudes of the line's history as well as the studies and project planning for its electrification. (orig.)

  19. East-West Couples: Distribution, Characteristics and Stability

    Directory of Open Access Journals (Sweden)

    Daniel Lois

    2014-12-01

    Full Text Available Der Originalbeitrag in deutscher Sprache ist verfügbar unter: Bd. 40 (2015: Ausgewählte deutsche Beiträge.SOEP data were used to examine relationships consisting of one partner socialised in West Germany and one in East Germany and who presently reside in the “old” (former West German or “new” (newly formed East German federal states. The estimated share of east-west couples among all marriages or cohabiting couples rises continuously within the observed period reaching approximately two and eleven percent respectively by 2009. The specific characteristics of east-west couples are that their employment-related division of labour is relatively egalitarian, above-average the partners are of different confessions and practice different religions, at least one of the partners is frequently divorced and there is also a strong tendency towards unmarried cohabitation. Besides the place of socialisation, the present place of residence has an independent impact on the economic situation, division of labour and marriage propensity. Analyses of relationship stability reveal that east-west couples exhibit a relatively high risk of separation. This is partly due to religious differences between the partners, but primarily to the low marriage propensity and the overrepresentation of divorced persons within this type of relationship.

  20. Safety of West Nile Virus vaccines in sandhill crane chicks

    Science.gov (United States)

    Olsen, G.H.; Miller, K.J.; Docherty, D.E.; Bochsler, V.S.; Folk, Martin J.; Nesbitt, Stephen A.

    2008-01-01

    West Nile virus arrived in North America in 1999 and has spread across the continent in the ensuing years. The virus has proven deadly to a variety of native avian species including sandhill cranes (Grus canadensis). In order to provide safe and efficacious protection for captive and released populations of whooping cranes (G. americana), we have conducted a series of four research projects. The last of these was a study of the effects of two different West Nile virus vaccines on young Florida sandhill crane (G. c. pratensis) chicks and subsequent challenge with the virus. We found that vaccinating crane chicks as early as day 7 post-hatch caused no adverse reactions or noticeable morbidity. We tested both a commercial equine vaccine West Nile - Innovator (Fort Dodge Laboratories, Fort Dodge, Iowa) and a new recombinant DNA vaccine (Centers for Disease Control). We had a 33% mortality in control chicks (n =6) from West Nile virus infection, versus 0% mortality in two groups of vaccinated chicks (n = 12), indicating the two vaccines tested were not only safe but effective in preventing West Nile virus.

  1. The lion in West Africa is critically endangered.

    Directory of Open Access Journals (Sweden)

    Philipp Henschel

    Full Text Available The African lion has declined to 500 km² PAs and compiled evidence of lion presence/absence for a further eight PAs. All PAs were situated within Lion Conservation Units, geographical units designated as priority lion areas by wildlife experts at a regional lion conservation workshop in 2005. Lions were confirmed in only 4 PAs, and our results suggest that only 406 (273-605 lions remain in West Africa, representing <250 mature individuals. Confirmed lion range is estimated at 49,000 km², or 1.1% of historical range in West Africa. PAs retaining lions were larger than PAs without lions and had significantly higher management budgets. We encourage revision of lion taxonomy, to recognize the genetic distinctiveness of West African lions and highlight their potentially unique conservation value. Further, we call for listing of the lion as critically endangered in West Africa, under criterion C2a(ii for populations with <250 mature individuals. Finally, considering the relative poverty of lion range states in West Africa, we call for urgent mobilization of investment from the international community to assist range states to increase management effectiveness of PAs retaining lions.

  2. Contraception and induced abortion in the West Indies: a review.

    Science.gov (United States)

    Boersma, A A; de Bruijn, J G M

    2011-10-01

    Most islands in the West Indies do not have liberal laws on abortion, nor laws on pregnancy prevention programmes (contraception). We present results of a literature review about the attitude of healthcare providers and women toward (emergency) contraception and induced abortion, prevalence, methods and juridical aspects of induced abortion and prevention policies. Articles were obtained from PubMed, EMBASE, MEDLINE, PsychlNFO and Soclndex (1999 to 2010) using as keywords contraception, induced abortion, termination of pregnancy, medical abortion and West Indies. Thirty-seven articles met the inclusion criteria: 18 on contraception, 17 on induced abortion and two on both subjects. Main results indicated that healthcare providers' knowledge of emergency contraception was low. Studies showed a poor knowledge of contraception, but counselling increased its effective use. Exact numbers about prevalence of abortion were not found. The total annual number of abortions in the West Indies is estimated at 300 000; one in four pregnancies ends in an abortion. The use of misoprostol diminished the complications of unsafe abortions. Legislation of abortion varies widely in the different islands in the West Indies: Cuba, Puerto Rico, Martinique, Guadeloupe and St Martin have legal abortions. Barbados was the first English-speaking island with liberal legislation on abortion. All other islands have restrictive laws. Despite high estimated numbers of abortion, research on prevalence of abortion is missing. Studies showed a poor knowledge of contraception and low use among adolescents. Most West Indian islands have restrictive laws on abortion.

  3. Geographic signatures of North American West Coast estuaries

    Science.gov (United States)

    Emmett, Robert; Llansó, Roberto; Newton, Jan; Thom, Ron; Hornberger, Michelle; Morgan, Cheryl; Levings, Colin; Copping, Andrea; Fishman, Paul

    2000-01-01

    West Coast estuaries are geologically young and composed of a variety of geomorphological types. These estuaries range from large fjords to shallow lagoons; from large to low freshwater flows. Natural hazards include E1 Niños, strong Pacific storms, and active tectonic activity. West Coast estuaries support a wide range of living resources: five salmon species, harvestable shellfish, waterfowl and marine birds, marine mammals, and a variety of algae and plants. Although populations of many of these living resources have declined (salmonids), others have increased (marine mammals). West Coast estuaries are also centers of commerce and increasingly large shipping traffic. The West Coast human population is rising faster than most other areas of the U.S. and Canada, and is distributed heavily in southern California, the San Francisco Bay area, around Puget Sound, and the Fraser River estuary. While water pollution is a problem in many of the urbanized estuaries, most estuaries do not suffer from poor water quality. Primary estuarine problems include habitat alterations, degradation, and loss; diverted freshwater flows; marine sediment contamination; and exotic species introductions. The growing West Coast economy and population are in part related to the quality of life, which is dependent on the use and enjoyment of abundant coastal natural resources.

  4. Soil investigation in cities of West Hungary

    Science.gov (United States)

    Bidló, A.; Szżcs, P.; Horváth, A.; Kámán, O.; Németh, E.

    2012-04-01

    The antropogene effects have an influence on the environment since the flow of materials and energy is continuous and mutual. In order to investigate the soil properly we have collected samples from sample spots in three West Hungarian cities (Sopron, Szombathely and Székesfehévár). The soil samples were taken from 0-10 and 10-20 cms depth in each city within a standard network and also at industrial territories. The number of the sampling spots were between 104 and 144, depending on the size of the city and the problematic places in town. We have noted the GPS-coordinates of the point, the elevation over sea level, the date of collecting, the type of the urban area, the information about land usage, vegetation, grass covering, the type of cover and the origin of soil. We described the following characters of soil: the borderline between two layers, the amount of humus, the structure, the compactness, the root system, the skeletal percent, the color, the physical assortment, the separated element and the soil defect. We measured the soil samples in laboratory according to the following parameters: acidity (pHH2O, pHKCl), lime content, hidrolitical and exchange acidity, amount of exchangeable cation, the humus content, the total nitrogen content, the ammonium-lactat-acetous acid (AL) solvent potassium and phosphorus content, the KCl solvent magnesium and calcium content, the strength of ethylene-diamin-tetra-acetous (EDTA) and diethylene-triamin-penta-acetous (DTPA) measurements of manganese, copper, zinc and iron element, as well as the particle size distribution. The results of field and laboratory investigation are represented in a GIS system. We attempted to draw a conclusion in connection with the condition, contamination and history of contamination of soil. The results showed that there is close connection between the soil parameters and geological conditions. However, this connection shows a decrease in the centre of the city. The acidity and calcic soils

  5. Cold war, quantum foundations, and East-West collaboration

    Energy Technology Data Exchange (ETDEWEB)

    Skaar-Jacobsen, Anja [Niels Bohr Archive, Copenhagen (Germany)

    2011-07-01

    It is well-known that the cultural cold war changed the ideological line in the Soviet Union from the late 1940s and that this had serious implications for the autonomy of research in genetics and quantum foundations in the East bloc. However, besides the more narrow concern from the point of view of research in quantum foundations, I suggest that the ideological impact on quantum foundations also constituted an obstacle for attempts by Western physicists to bring about a rapprochement between physics in the east and west in general. In connection with re-establishing East-West co-operation between physicists after Stalin's death in 1953 this obstacle needed to be cleared away. In my talk I discuss these issues and how the Institute for Theoretical Physics in Copenhagen came to be an important meeting place for physicists from the East and West from the mid-1950s.

  6. Reconfiguring the West in Neil Jordan's short story "Love"

    Directory of Open Access Journals (Sweden)

    Stipe Grgas

    2004-12-01

    Full Text Available In introducing his article,  the author draws attention to the enormous importance space, especially the west of Ireland, has in the Irish culture and in the legitimization of Irish identity. The central part of the article is devoted to an analysis of Neil Jordan's  "A Love" in which the author describes the way Jordan uses the West as the privileged place of Irish self-representation. A close reading of the text shows the writer's strategies which subvert the conventional image of the West of Ireland  and the author voices the opinion that Jordan's text refers to the end of one conceptualization of Irish identity. Offering the concept of heterotopia (Foucault in his conclusion the author opens up the possibility of reading the representation of space in Jordan's story as his inscription of those places which were elided from the utopian Irish national saga.

  7. A Postmodernist Reading of Sam Shepard’s True West

    Directory of Open Access Journals (Sweden)

    Abdol Hossein Joodaki

    2013-09-01

    Full Text Available This paper attempts to render some vivid postmodernist features in Shepard’s True West (1980, which is inspired by myths of American life and popular culture. Shepard’s True West suggests so many interesting postmodern elements. With a departure from conventional norms of character, dialogue and narrative; the elements of pastiche, subjective irony, and savage humor have become hallmarks of most of his works. These features and some of the basic traces of postmodern literature, including Lyotard’s theory of the end of meta-narratives and language game, Derrida’s deconstruction and Baudrillard’s simulation, as well as language fragmentation, uncertainty and duality,  altogether, have given Shepard’s True West a postmodern atmosphere. Keywords: Postmodernist Literature; Grand Narratives; Duality; Self Reflexivity; Language Game; Binary oppositions; Simulacrum

  8. Rod consolidation at the West Valley Demonstration Project

    Energy Technology Data Exchange (ETDEWEB)

    Bailey, W.J.

    1986-12-01

    A rod consolidation demonstration with irradiated pressurized water reactor fuel was recently conducted by personnel from Nuclear Assurance Corporation and West Valley Nuclear Services Company at the West Valley Demonstration Project in West Valley, New York. The rod consolidation demonstration involved pulling all of the fuel rods from six fuel Assemblies. In general, the rod pulling proceeded smoothly. The highest compaction ratio attained was 1:8:1. Among the total of 1074 fuel rods were some known degraded rods (they had collapsed cladding, a result of in-reactor fuel densification), but no rods were broken or dropped during the demonstration. One aim was to gather information on the effect of rod consolidation operations on the integrity of the fuel rods during subsequent handling and storage. Another goal was to collect information on the condition and handling of intact, damaged, and failed fuel that has been in storage for an extended period. 9 refs., 8 figs., 1 tab.

  9. Climate variability in West Greenland during the past 1500 years

    DEFF Research Database (Denmark)

    dos Santos Ribeiro, Sofia Isabel; Moros, Matthias; Ellegaard, Marianne;

    2012-01-01

    document late-Holocene climate variability in West Greenland as inferred from a marine sediment record from the outer Disko Bay. Organic-walled dinoflagellate cysts and other palynomorphs were used to reconstruct environmental changes in the area through the last c. 1500 years at 30–40 years resolution....... Sea ice cover and primary productivity were identified as the two main factors driving dinoflagellate cyst community changes through time. Our data provide evidence for an opposite climate trend in West Greenland relative to the NE Atlantic region from c. AD 500 to 1050. For the same period, sea......Ribeiro, S., Moros, M., Ellegaard, M. & Kuijpers, A. 2012 (January): Climate variability in West Greenland during the past 1500 years: evidence from a high-resolution marine palynological record from Disko Bay. Boreas, Vol. 41, pp. 68–83. 10.1111/j.1502-3885.2011.00216.x. ISSN 0300-9483. Here we...

  10. Genetic structure of West Greenland populations of lumpfish Cyclopterus lumpus

    DEFF Research Database (Denmark)

    Mayoral, Elsa Garcia; Olsen, M.; Hedeholm, R.

    2016-01-01

    In this study, 11 microsatellite markers were used to determine the structure of West Greenlandic lumpfish Cyclopterus lumpus populations across six spawning locations spanning >1500 km and compared with neighbouring populations in Canada and Iceland. To evaluate whether data allow for identifica......In this study, 11 microsatellite markers were used to determine the structure of West Greenlandic lumpfish Cyclopterus lumpus populations across six spawning locations spanning >1500 km and compared with neighbouring populations in Canada and Iceland. To evaluate whether data allow...... for identification of origin of C. lumpus in Greenlandic waters, genetic assignment analysis was performed for 86 C. lumpus sampled on a feeding migration. Significant structuring with isolation by distance was observed in the West Greenland samples and two major subpopulations, north and south, were suggested...

  11. The lion in West Africa is critically endangered.

    Science.gov (United States)

    Henschel, Philipp; Coad, Lauren; Burton, Cole; Chataigner, Beatrice; Dunn, Andrew; MacDonald, David; Saidu, Yohanna; Hunter, Luke T B

    2014-01-01

    The African lion has declined to Africa, where the species is considered regionally endangered. Elevating their conservation significance, recent molecular studies establish the genetic distinctiveness of West and Central African lions from other extant African populations. Interventions to save West African lions are urgently required. However formulating effective conservation strategies has been hampered by a lack of data on the species' current distribution, status, and potential management deficiencies of protected areas (PAs) harboring lions. Our study synthesized available expert opinion and field data to close this knowledge gap, and formulate recommendations for the conservation of West African lions. We undertook lion surveys in 13 large (>500 km²) PAs and compiled evidence of lion presence/absence for a further eight PAs. All PAs were situated within Lion Conservation Units, geographical units designated as priority lion areas by wildlife experts at a regional lion conservation workshop in 2005. Lions were confirmed in only 4 PAs, and our results suggest that only 406 (273-605) lions remain in West Africa, representing Africa. PAs retaining lions were larger than PAs without lions and had significantly higher management budgets. We encourage revision of lion taxonomy, to recognize the genetic distinctiveness of West African lions and highlight their potentially unique conservation value. Further, we call for listing of the lion as critically endangered in West Africa, under criterion C2a(ii) for populations with Africa, we call for urgent mobilization of investment from the international community to assist range states to increase management effectiveness of PAs retaining lions.

  12. Vitrification facility at the West Valley Demonstration Project

    Energy Technology Data Exchange (ETDEWEB)

    DesCamp, V.A.; McMahon, C.L.

    1996-07-01

    This report is a description of the West Valley Demonstration Project`s vitrification facilities from the establishment of the West Valley, NY site as a federal and state cooperative project to the completion of all activities necessary to begin solidification of radioactive waste into glass by vitrification. Topics discussed in this report include the Project`s background, high-level radioactive waste consolidation, vitrification process and component testing, facilities design and construction, waste/glass recipe development, integrated facility testing, and readiness activities for radioactive waste processing.

  13. Developing Integrated Care Teams Across the North West London System

    OpenAIRE

    O'Halloran, Katherine Anne

    2016-01-01

    The health needs of the North West London population are changing. People are generally living longer and as a result a growing number are suffering from complex, long-term health conditions. This inevitably creates pressure on available services, to the point where there is a need to look at how these can be better provided. In North West London, the way hospitals and community health services are provided is being transformed to deliver a more co-ordinated and person centred service, achiev...

  14. Immunological disturbance in West and Lennox-Gastaut syndromes

    Directory of Open Access Journals (Sweden)

    Terezinha C. B. Montelli

    1984-06-01

    Full Text Available Cell-mediated and humoral immunity were investigated in 18 patients with West syndrome, 12 with Lennox-Gastaut syndrome and 19 healthy controls. The study included determination of Ô and  peripheral blood lymphocytes, serum levels of IgG, IgA and IgM, skin sensibilization with DNCB, intracutaneous PHA, leucocyte migration inhibition test and lymphocyte blastic transformation in the presence of PHA. Cell-mediated deficiency was detected in 28 children whereas low levels of immunoglobulins were observed only in 6 children. Immunological disturbances were more prominent in children with West syndrome.

  15. CHARACTERISTICS OF SMALL-SCALE ENTERPRISES IN WEST SUMATRA

    Directory of Open Access Journals (Sweden)

    Syahrial Syarif

    2004-01-01

    Full Text Available Small-scale enterprises in West Sumatra experienced slow growth and some constraints in their development process. In 1998, small businesses represented 99.61% of total business units operating in West Sumatra and absorbed 88.8% of total employment. The orientation of their businesses mostly on domestic market although recently they enter international market. Export contribution of small businesses still very low but growing steadily during the last three years. This research showed that enterprises dealing with embroideries, garments and handicrafts are ambitiously orienting their products towards foreign markets. At the same time these business categories are also proven to be the most profitable businesses.

  16. Satellite Tracking of Humpback Whales in West Greenland

    DEFF Research Database (Denmark)

    Dietz, R.; Teilmann, J.; Heide-Jørgensen, M.-P.

    In June 2000, 6 humpback whales (Megaptere novaeangliae) were tagged with satellite transmitters off West Greenland. Contact remained for up to 42 days. The tagging revealed that within the month of June, humpback whales off West Greenland moved between Fiskenæs Banke, Fyllas Banke, Tovqussaq Banke......, Sukkertop Banke and Lille Hellefiske Banke. The whales showed a preference for the continental slopes with depths less than 200 m, however, few dives were recorded down to 500 m. The whales had a preference for dives lasting 7-8 min. (15%) and no dives lasted longer than 15 min....

  17. Distribution and abundance of West Greenland humpback whales ( Megaptera novaeangliae )

    DEFF Research Database (Denmark)

    Larsen, Finn; Hammond, P.S.

    2004-01-01

    Photo-identification surveys of humpback whales Megaptera novaeangliae were conducted at West Greenland during 1988-93, the last 2 years of which were part of the internationally coordinated humpback whale research programme YoNAH, with the primary aim of estimating abundance for the West Greenland...... effort. A total of 670 groups of humpback whales was encountered leading to the identification of 348 individual animals. Three areas of concentration were identified: an area off Nuuk; an area at c. 63degrees30'N; and an area off Frederikshab. Sequential Petersen capture-recapture estimates of abundance...

  18. West Valley Demonstration Project site environmental report, calendar year 1999

    Energy Technology Data Exchange (ETDEWEB)

    None Available

    2000-06-01

    This report represents a single, comprehensive source of off-site and on-site environmental monitoring data collected during 1999 by environmental monitoring personnel for the West Valley Demonstration Project (WVDP), West Valley, New York. The environmental monitoring program and results are discussed in the body of this report. The monitoring data are presented in the appendices. The data collected provide an historical record of radionuclide and radiation levels from natural and manmade sources in the survey area and document the quality of the groundwater on and around the WVDP and the quality of the air and water discharged by the WVDP.

  19. West Valley Demonstration Project site environmental report, calendar year 1997

    Energy Technology Data Exchange (ETDEWEB)

    None

    1998-06-01

    This report represents a single, comprehensive source of off-site and on-site environmental monitoring data collected during 1997 by environmental monitoring personnel for the West Valley Demonstration Project (WVDP), West Valley, New York. The environmental monitoring program and results are discussed in the body of this report. The monitoring data are presented in the appendices. The data collected provide an historical record of radionuclide and radiation levels from natural and manmade sources in the survey area and document the quality of the groundwater on and around the WVDP and the quality of the air and water discharged by the WVDP.

  20. West Valley Demonstration Project site environmental report calendar year 1998

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-06-01

    This report represents a single, comprehensive source of off-site and on-site environmental monitoring data collected during 1998 by environmental monitoring personnel for the West Valley Demonstration Project (WVDP), West Valley, New York. The environmental monitoring program and results are discussed in the body of this report. The monitoring data are presented in the appendices. The data collected provide an historical record of radionuclide and radiation levels from natural and manmade sources in the survey area and document the quality of the groundwater on and around the WVDP and the quality of the air and water discharged by the WVDP.

  1. West Mining Expanding Into Lead Zinc Smelting Industry

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    <正>West Mining,China’s 2nd largest lead zinc miner(only next to Yunnan Jinding Zinc with an annual lead and zinc output of approx. 180,000 tons in metal content),has been put- ting efforts on the control of resources for years.Additionally,it has recently increased its investment on smelting business by holding shares of Bayanzhuoer Zijin for more zinc smelting asset.West Mining has just com- pleted the construction of its 60,000-ton zinc

  2. PREVALENCE OF BOVINE VIRAL DIARRHOEA VIRUS IN WEST BENGAL, INDIA

    Directory of Open Access Journals (Sweden)

    Reshmi Ghosh

    2015-12-01

    Full Text Available Bovine Viral Diarrhea (BVD is one of the most economically important diseases in cattle. The present study was undertaken to diagnose the persistently infected (PI animals by AntigenELISA and Reverse Transcriptase PCR using serum samples from organized farms as well as rural areas of West Bengal. The results showed that out of 964 serum samples tested 07 (0.73% was positive for BVDV by Antigen-ELISA. For further confirmation, RNA was extracted from the positive samples and RT-PCR was performed with 5' UTR specific primers which showed 294 bp amplicons. This finding showed circulation of BVDV in cattle in West Bengal, India.

  3. The Dendrochronological Dating of Timber Crossings in West Jutland, Denmark

    DEFF Research Database (Denmark)

    Daly, Aoife

    2006-01-01

    The dendrochronological analyses of a large number of timbers from two sites are described. Nybro causeway in west Jutland, Denmark, which was built in the 8th century AD, consists of numerous phases, identifiable due to the large number of dendrochronologically dated timbers, of which many had...... complete sapwood, to bark edge, preserved. Skjern Bridge, across the Skjern River and also in west Jutland, is medieval in date, with phases from the 12th to 14th centuries. The bridge is one element in a complex of activity by the river including an earlier ford, a fish weir contemporary with the bridge...

  4. Deglacial History and Paleoceanography of the Umanak System, West Greenland

    DEFF Research Database (Denmark)

    Sheldon, Christina; Jennings, Anne; Andrews, John

    2012-01-01

    is to document the timing and rate of ice retreat and the role of the West Greenland Current (WGC) in initiating or sustaining ice retreat. During cruise JR175 of the UK ship RRS James Clark Ross in 2009, sediment cores and geophysical data were collected from the Umanak System to document the glacial history......A record of Greenland Ice Sheet (GIS) and ice-stream history, ice-sheet ocean interactions and paleoclimate from the Last Glacial Maximum to present is preserved in the sediments of the Umanak fjord, shelf trough and trough mouth fan system of central West Greenland. The objective of this study...

  5. Satellite Tracking of Humpback Whales in West Greenland

    DEFF Research Database (Denmark)

    Dietz, R.; Teilmann, J.; Heide-Jørgensen, M.-P.

    In June 2000, 6 humpback whales (Megaptere novaeangliae) were tagged with satellite transmitters off West Greenland. Contact remained for up to 42 days. The tagging revealed that within the month of June, humpback whales off West Greenland moved between Fiskenæs Banke, Fyllas Banke, Tovqussaq Banke......, Sukkertop Banke and Lille Hellefiske Banke. The whales showed a preference for the continental slopes with depths less than 200 m, however, few dives were recorded down to 500 m. The whales had a preference for dives lasting 7-8 min. (15%) and no dives lasted longer than 15 min....

  6. Research needs for lion conservation in West and Central Africa.

    Science.gov (United States)

    Bauer, Hans; De Iongh, Hans H; Princée, Frank P; Ngantou, Daniel

    2003-08-01

    The lion has historically probably been widespread at low densities in West and Central Africa, nowadays they are largely restricted to small isolated populations inside protected areas. The total number is probably between 1200 and 2700, the best possible guesstimate would be 1700. Mankind is the main cause for the suspected decline of lion populations, both inside and outside protected areas. Very little research has been done on West and Central African lions a few examples are summarized here. The international community is slowly becoming aware of threats to lions in the region and some initiatives for lion conservation have started.

  7. Antibodies to henipavirus or henipa-like viruses in domestic pigs in Ghana, West Africa.

    Directory of Open Access Journals (Sweden)

    David T S Hayman

    Full Text Available Henipaviruses, Hendra virus (HeV and Nipah virus (NiV, have Pteropid bats as their known natural reservoirs. Antibodies against henipaviruses have been found in Eidolon helvum, an old world fruit bat species, and henipavirus-like nucleic acid has been detected in faecal samples from E. helvum in Ghana. The initial outbreak of NiV in Malaysia led to over 265 human encephalitis cases, including 105 deaths, with infected pigs acting as amplifier hosts for NiV during the outbreak. We detected non-neutralizing antibodies against viruses of the genus Henipavirus in approximately 5% of pig sera (N = 97 tested in Ghana, but not in a small sample of other domestic species sampled under a E. helvum roost. Although we did not detect neutralizing antibody, our results suggest prior exposure of the Ghana pig population to henipavirus(es. Because a wide diversity of henipavirus-like nucleic acid sequences have been found in Ghanaian E. helvum, we hypothesise that these pigs might have been infected by henipavirus(es sufficiently divergent enough from HeVor NiV to produce cross-reactive, but not cross-neutralizing antibodies to HeV or NiV.

  8. Teaching in West Africa: Dig beneath the Surface

    Science.gov (United States)

    Briam, Carol

    2007-01-01

    In this article, the author shares her experience teaching English to adult learners at the American Cultural Center in Dakar, Senegal, a poor, primarily Muslim country and former French colony in West Africa. Her class was composed of about 30 students, whose age ranged from about 18 to 50. They were mostly men and mostly Senegalese, along with a…

  9. Walruses in West Greenland: Where do they belong?

    DEFF Research Database (Denmark)

    Born, E. W.; Andersen, L. W.; Dietz, R.

    ) transmitted for more than 3 months during which time she moved from CWG west to SE Baffin Island (SE BI). Hence, the studies supported a suspected connection between walruses in CWG and the HS-SE BI area. Future studies include a genetic comparison of biopsies from SE BI and CWG, and deployment of satellite...

  10. Tourism package preferences of West Virginia state park visitors

    Science.gov (United States)

    William Gravley; John Dengler; Roy Ramthun; Chad Pierskalla

    2009-01-01

    This study was a preliminary examination of the activity and spending behavior of visitors to Pipestem State Park in West Virginia. This state park is being used as a case study area to determine whether a new fish stocking program accompanied by appropriate marketing activities can increase park visitation by anglers and other sports-oriented people. The research was...

  11. Future of West African Monsoon in A Warming Climate

    Science.gov (United States)

    Raj, Jerry; Kunhu Bangalath, Hamza; Stenchikov, Georgiy

    2016-04-01

    West Africa is the home of more than 300 million people whose agriculture based economy highly relies on West African Monsoon (WAM), which produces a mean annual rainfall of 150 - 2,500 mm and variability and change of which have devastating impact on the local population. The observed widespread drought in West Africa during the 1970s and 1980s was the most significant drought at regional scale during the twentieth century. In this study, a high resolution AGCM, High Resolution Atmospheric Model (HiRAM), is used to study the effects of anthropogenic greenhouse warming on WAM. HiRAM is developed at GFDL based on AM2 and employs a cubed-sphere finite volume dynamical core and uses shallow convective scheme (for moist convection and stratiform cloudiness) instead of deep convective parameterization. Future projections are done using two representative concentration pathways, RCP 4.5 and RCP 8.5 from 2007 to 2050 at C360 (~25 km) resolution. Both RCP 4.5 and RCP 8.5 scenarios predict warming over West Africa during boreal summer, especially over Western Sahara. Also, both scenarios predict southward shift in WAM rainfall pattern and drying over Southern Sahara, while RCP 8.5 predicts enhanced rainfall over Gulf of Guinea. The intensification of rainfall over tropical latitudes is caused by increased low level winds due to warm SST over Gulf of Guinea.

  12. Lassa fever in West African sub-region: an overview

    Directory of Open Access Journals (Sweden)

    O. Ogbu

    2007-01-01

    Full Text Available Lassa fever is an acute viral zoonotic illness caused by Lassa virus, an arenavirus known to beresponsible for a severe haemorrhagic fever characterised by fever, muscle aches, sore throat, nausea,vomiting and, chest and abdominal pain. The virus exhibits persistent, asymptomatic infection withprofuse urinary virus excretion in the ubiquitous rodent vector, Mastomys natalensis. Lassa fever isendemic in West Africa and has been reported from Sierra Leone, Guinea, Liberia, and Nigeria. Somestudies indicate that 300,000 to 500,000 cases of Lassa fever and 5000 deaths occur yearly across WestAfrica. Studies reported in English, that investigated Lassa fever with reference to West Africa wereidentified using the Medline Entrez-PubMed search and were used for this review. The scarcity ofresources available for health care delivery system and the political instability that characterise theWest African countries would continue to impede efforts for the control of Lassa fever in the sub-region.There is need for adequate training of health care workers regarding diagnostics, intensive care ofpatients under isolation, contact tracing, adequate precautionary measures in handling infectiouslaboratory specimens, control of the vector as well as care and disposal of infectious waste.

  13. Abiotic factors influencing deer browsing in West Virginia

    Science.gov (United States)

    Tyler A. Campbell; Benjamin R. Laseter; W. Mark Ford; Richard H. Odom; Karl V. Miller

    2006-01-01

    We present a comparison of woody browse availability and white-tailed deer (Odocoileus virginianus) use among clearcut interiors, skidder trail edges, and mature forest and an evaluation of the relative importance of aboitic factors in predicting browsing pressure within regenerating clearcuts in the central Appalachians of West Virginia. We sampled...

  14. A century of glacier change in the American West

    Science.gov (United States)

    Fountain, A. G.

    2007-12-01

    Over the past 100 years glaciers in the American West (exclusive of Alaska) have largely receded. The magnitude of the recession varies across the west, with the greatest loss in Montana and California (>50% area loss) and the least loss on the stratovolcanoes (>35%) of the Pacific Northwest. The variations can be broadly characterized by elevation. Our results suggest that increased mass loss caused by increased summer temperatures affect all glaciers, whereas increasing winter temperatures, that change the phase of precipitation from snow to rain adversely affect those glaciers less than 3000m in elevation. The high glaciers (>3000m) of California and Colorado appear to be immune to variations in snowfall making them sensitive to variations in temperature alone. We infer that these very small, steep glaciers can only hold a given amount of snow beyond which extra snow avalanches or is blown off. Conversely, during winters of little direct snowfall, additional snow may be added through win drift from the surrounding terrain. The relatively little glacier shrinkage on the stratovolcanoes is due to the high altitude of the glacier accumulation zones. An east to west decrease in glacier shrinkage from Montana through Washington is due to enhanced winter precipitation along the west coast that somewhat buffers ice loss due to summer temperatures and winter precipitation phase changes.

  15. Energy in the Mountain West: Colonialism and Independence

    Energy Technology Data Exchange (ETDEWEB)

    Steven Piet; Lloyd Brown; Robert Cherry; Craig Cooper; Harold Heydt; Richard Holman; Travis McLing

    2007-08-01

    In many ways, the mountain west (Alaska, Arizona, Colorado, Idaho, Montana, New Mexico, Nevada, Utah, Wyoming) is an energy colony for the rest of the United States: it is rich in energy resources that are extracted to fuel economic growth in the wealthier and more populous coastal regions. Federal agencies and global corporations often behave as if the mountain west is a place to be exploited or managed for the benefit of customers and consumers elsewhere. Yet, the area. is not vast empty space with a limitless supply of natural resources, but rather a fast-growing region with a diverse economic base dependent on a limited supply of water. New decision processes and collaborations are slowly changing this situation, but in a piecemeal fashion that places local communities at odds with powerful external interests. Proper planning of major development is needed to insure that the west has a strong economic and cultural future after the fossil energy resources decline, even if that might be a century from now. To encourage the necessary public discussions, this paper identifies key differences between the mountain west and the rest of the United States and suggests some holistic approaches that could improve our future. This paper is designed to provoke thought and discussion; it does not report new analyses on energy resources or usage. It is a summary of a large group effort.

  16. Development of a regional food composition table for West Africa.

    Science.gov (United States)

    Stadlmayr, Barbara; Charrondière, U Ruth; Burlingame, Barbara

    2013-10-01

    Knowledge of the nutrient content of foods is fundamental for virtually all nutrition-related projects, programmes and policies. Low quality compositional data may lead to inappropriate policies and funds spent unnecessarily. Existing food composition tables (FCT) for most West African countries date back to 1960 and 1970 and contain in general few foods and components without documentation. As a result of the recommendations by the Economic Community of West African States (ECOWAS) Nutrition forum and other high level meetings, FAO/INFOODS, WAHO/ECOWAS and Bioversity International developed the West African FCT. It contains 472 foods and 28 components. Emphasis was given to include data on food biodiversity by incorporating cultivars/varieties and underutilized foods. The West African FCT enables users to address diet-related health problems, strengthen local development, enhance trade and promote biodiversity. In addition it contributes to poverty alleviation in both rural and urban areas. The FCT needs to be updated regularly and it is the most recent example of INFOODS for regional food composition activities. Copyright © 2012 The Food and Agriculture Organization of the United Nations (FAO). Published by Elsevier Ltd.. All rights reserved.

  17. West-to-East Pipeline Entirely Comes under Construction

    Institute of Scientific and Technical Information of China (English)

    Zhang Zhicheng

    2002-01-01

    @@ PetroChina West-to-East Pipleline Company announced at a press conference held in February that the Chinese Government has formally approved the feasibility study report of the nation-crossing pipeline, marking that the 4200-kilometer pipeline project was brought under construction.

  18. Using Radio To Deliver Teacher Training in West Africa.

    Science.gov (United States)

    Norman, Douglas

    Three radio stations serving the interior of Liberia, West Africa, were used to broadcast inservice training programs to teachers in grades 1-6. Most of the teachers had not attended college and had received only 5 weeks of preservice training before going into the classroom. Poor roads, a 6-month wet season, and lack of vehicles made face-to-face…

  19. Understanding the productivity of cassava in West Africa

    NARCIS (Netherlands)

    Ezui, Kodjovi Senam

    2017-01-01

    Drought stress and sub-optimal soil fertility management are major constraints to crop production in general and to cassava (Manihot esculenta Crantz) in particular in the rain-fed cropping systems in West Africa. Cassava is an important source of calories for millions of smallholder households in s

  20. Visitors' perceptions of tourism development in West Virginia

    Science.gov (United States)

    Jinyang Deng; Maureen Young Bender

    2008-01-01

    Previous studies indicate that tourists' destination choices are increasingly influenced by perceptions of sustainability but research into tourists' insights and sensitivities about sustainability is lacking. This study examines how visitors to West Virginia perceive tourism development in the state. Findings indicate that visitors' perceptions are...

  1. Clinical sentinel surveillance of equine West Nile fever, Spain

    DEFF Research Database (Denmark)

    Saegerman, C.; Alba-Casals, A.; García-Bocanegra, I.;

    2016-01-01

    West Nile fever (WNF) is a viral zoonotic infection caused by a mosquito-borne flavivirus of the Flaviviridae family. According to a comparative study, the passive surveillance of horses by equine veterinarians appeared to be the most cost-effective system in the European context of WNF. Clinical...

  2. Education and Happiness: Perspectives of the East and the West

    Science.gov (United States)

    Lee, Jeong-Kyu

    2008-01-01

    The purpose of this paper is to investigate the relations between education and happiness from the perspectives of the West and the East, especially Buddhism and Christianity as well as classical Greek and Chinese great thinkers. In order to examine this study systematically, three research questions are addressed as follows: First, what are…

  3. A new genus of arboreal rat from West Java, Indonesia

    NARCIS (Netherlands)

    Musser, G.G.

    1981-01-01

    Kadarsanomys nov. gen. is proposed for Rattus sodyi Bartels, 1937, and contrasted with Rattus and Lenothrix, two genera with which sodyi has been closely connected in the past. Kadarsanomys sodyi is an arboreal rat associated with bamboo on the forested volcanoes of West Java. Kadarsanomys has no cl

  4. Lassa fever in West African sub-region: an overview.

    Science.gov (United States)

    Ogbu, O; Ajuluchukwu, E; Uneke, C J

    2007-03-01

    Lassa fever is an acute viral zoonotic illness caused by Lassa virus, an arenavirus known to be responsible for a severe haemorrhagic fever characterised by fever, muscle aches, sore throat, nausea, vomiting and, chest and abdominal pain. The virus exhibits persistent, asymptomatic infection with profuse urinary virus excretion in the ubiquitous rodent vector, Mastomys natalensis. Lassa fever is endemic in West Africa and has been reported from Sierra Leone, Guinea, Liberia, and Nigeria. Some studies indicate that 300,000 to 500,000 cases of Lassa fever and 5000 deaths occur yearly across West Africa. Studies reported in English, that investigated Lassa fever with reference to West Africa were identified using the Medline Entrez-PubMed search and were used for this review. The scarcity of resources available for health care delivery system and the political instability that characterise the West African countries would continue to impede efforts for the control of Lassa fever in the sub-region. There is need for adequate training of health care workers regarding diagnostics, intensive care of patients under isolation, contact tracing, adequate precautionary measures in handling infectious laboratory specimens, control of the vector as well as care and disposal of infectious waste.

  5. Cycles of Protest in West Germany: Experiences from Three Decades.

    Science.gov (United States)

    Mushaben, Joyce Marie

    The effects of the West German peace movement over the past 30 years have led to the development of a new concept of political participation in that country. Since 1950, a proliferation of protest movements has reflected a wide range of ideological, social, and geographic perspectives. Pacifist Protestants and the German Trade Union began sporadic…

  6. Genetic relationships among West African okra (Abelmoschus caillei ...

    African Journals Online (AJOL)

    STORAGESEVER

    2008-05-16

    May 16, 2008 ... Key words: West African okra, genetic relationship, Abelmoschus caillei, ... has long been a major goal in evolutionary biology. Infor- ... management as a quick, cost-effective and reliable ... affected by environment (Staub et al., 1997). ... Supernatant containing DNA was transferred into a new 2 ml U –.

  7. Agriculture, livelihoods and climate change in the West African Sahel

    NARCIS (Netherlands)

    Sissoko, K.; Keulen, van H.; Verhagen, A.; Tekken, V.; Battaglini, A.

    2011-01-01

    The West African Sahel is a harsh environment stressed by a fast-growing population and increasing pressure on the scarce natural resources. Agriculture is the main source of livelihood of the majority of the people living in the area. Increases in temperature and/or modifications in rainfall quanti

  8. Nearshore currents along the Karnataka coast, west coast of India

    Digital Repository Service at National Institute of Oceanography (India)

    SanilKumar, V.; Dora, G.U.; Philip, C.S.; Pednekar, P.S.; Singh, J.

    Measured current data at 7 locations and tide data at 3 locations during the pre-summer monsoon period along the west coast of India is used in the study. The surface currents during March showed a predominant northward trend and during April...

  9. The extinction of the West African lion: whose responsibility?

    NARCIS (Netherlands)

    Nollkaemper, A.

    2014-01-01

    A recently published study showed that the lion in West Africa is now critically endangered and faces extinction. From one angle, this would be just one of the large (though unknown) number of species that has previously faced extinction or has even become extinct. But the risk of extinction of some

  10. Septic Abortion Managed in a Tertiary Hospital in West Bengal

    African Journals Online (AJOL)

    a cross-sectional study carried out in Burdwan Medical College and Hospital in West Bengal between July 2011 and June 2012. On ... The modal age group of the patients was 15-25 years (49.5%). Majority of ... Address for correspondence.

  11. Beach rocks of the central west coast of India

    Digital Repository Service at National Institute of Oceanography (India)

    Wagle, B.G.

    in the intertidal zone or spray zone as well as 2 to 4 m above the present high 112 Iv t l<'igure 1. Map showing the location of beach rock exposures along the central west Clnst of India. water line. They occur as medium thick beds (3-4 m), more often as patches...

  12. West Africa Land Use Land Cover Time Series

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This series of three-period land use land cover (LULC) datasets (1975, 2000, and 2013) aids in monitoring change in West Africa’s land resources (exception is...

  13. Bridging East and West Educational Divides in Singapore

    Science.gov (United States)

    Kumar, Prem

    2013-01-01

    In Asia, we are witnessing an era where the pendulum of power is swaying towards the East with the rising economic strength of China and India. Singapore is at the "crossroads" between the East and West of these most populous nations on earth. Although Singapore may appear the most Westernised country in Asia, she is nevertheless a multicultural…

  14. The extinction of the West African lion: whose responsibility?

    NARCIS (Netherlands)

    A. Nollkaemper

    2014-01-01

    A recently published study showed that the lion in West Africa is now critically endangered and faces extinction. From one angle, this would be just one of the large (though unknown) number of species that has previously faced extinction or has even become extinct. But the risk of extinction of some

  15. Virtual Reference Service in Academic Libraries in West Africa

    Science.gov (United States)

    Sekyere, Kwabena

    2011-01-01

    As technology continues to advance, libraries in Europe and America continue to improve upon their virtual reference services by employing new Web technologies and applying them to existing services. West African academic libraries have begun providing resources electronically to their users but still typically lag behind in the services they…

  16. The Shallow-water Octocorallia of the West Indian Region

    NARCIS (Netherlands)

    Bayer, Frederick M.

    1961-01-01

    The alcyonarian fauna of the West Indies is prolific and conspicuous and has been known for many years, with the natural result that a great many more species have been described than actually exist. The deep-water fauna, which received little attention prior to the work of VERRILL, was thoroughly r

  17. Turf varieties for the Intermountain West - today and tomorrow

    Science.gov (United States)

    Landscape managers and sod growers in the Intermountain West are limited in their ability to select the varieties of turfgrass that perform well in this semi-arid environment. The varieties of seed sold in Utah are rarely based on what performs well here. Turf managers will often make one visit to...

  18. Outcome of Radioiodine Therapy in a West African Population

    NARCIS (Netherlands)

    Onimode, Yetunde A; Ankrah, Alfred; Adedapo, Kayode S

    2016-01-01

    Hyperthyroidism continues to be a pressing public health concern in West Africa. Its prevalence in Africa has been quoted as 1.2%-9.9%, with Graves' disease as its most common cause. Radioiodine-131 (RAI) therapy of hyperthyroidism recently commenced in two government hospitals in Ghana and Nigeria.

  19. Local perceptions of migration from north-west Ghana

    NARCIS (Netherlands)

    van der Geest, K.

    2010-01-01

    The Upper West Region in northern Ghana is a major source area of migrants who travel to southern Ghana seasonally or for longer periods. This has important implications for the lives and livelihoods of the migrants themselves and their relatives at home. Almost invariably the impact of out-migratio

  20. On the origin of the West Indian Fauna

    NARCIS (Netherlands)

    Scharff, R.F.

    1922-01-01

    Many zoologists have attempted to solve this problem which is a complicated one. In one respect the views of almost all agree. It is in the belief that the West Indian islands must have undergone profound alterations in configuration during the past. It is thought that at one time they must all have

  1. The Buzz-z-z on West Nile Virus

    Centers for Disease Control (CDC) Podcasts

    2012-01-12

    In this podcast for kids, the Kidtastics talk about West Nile Virus and how to protect yourself from it.  Created: 1/12/2012 by Centers for Disease Control and Prevention (CDC).   Date Released: 1/12/2012.

  2. 78 FR 15292 - Drawbridge Operation Regulations; West Bay, Osterville, MA

    Science.gov (United States)

    2013-03-11

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HOMELAND SECURITY Coast Guard 33 CFR Part 117 Drawbridge Operation Regulations; West Bay, Osterville, MA AGENCY: Coast Guard, DHS. ACTION: Notice of temporary deviation from regulation. SUMMARY: The Coast Guard...

  3. Organic complexation of iron in the West Atlantic Ocean

    NARCIS (Netherlands)

    Gerringa, L.J.A.; Rijkenberg, M.J.; Schoemann, V.; Laan, P.; de Baar, H.J.W.

    2015-01-01

    The characteristics of the dissolved iron (DFe) binding organic ligands were determined during 3 Dutch GEOTRACES cruises covering the length of the West Atlantic Ocean. Adsorptive Differential Pulse Cathodic Stripping Voltammetry (AdDPCSV) with TAC as competing ligand was used to measure Fe binding

  4. Moral Self-Cultivation East and West: A Critique

    Science.gov (United States)

    Slote, Michael

    2016-01-01

    Moral Self-Cultivation plays an important, even a central role, in the Confucian philosophical tradition, but philosophers in the West, most notably Aristotle and Kant, also hold that moral self-cultivation or self-shaping is possible and morally imperative. This paper argues that these traditions are psychologically unrealistic in what they say…

  5. Malignant lymphoma in a west Indian manatee (Trichechus manatus)

    DEFF Research Database (Denmark)

    Hammer, Anne Sofie; Klausen, Bjarne; Knold, Steffen

    2005-01-01

    We identified a malignant lymphoma infiltrating the lung, liver, kidney, mesenteric lymph nodes, and eye as the cause of death in a male West Indian manatee (Trichechus manatus). Diagnosis was based on gross histopathologic, and immunohistochemical studies. Tissue samples from ten organs were inc...

  6. West Valley transfer cart control system design description

    Energy Technology Data Exchange (ETDEWEB)

    Bradley, E.C.; Crutcher, R.I.; Halliwell, J.W.; Hileman, M.S.; Moore, M.R.; Nodine, R.N.; Ruppel, F.R.; Vandermolen, R.I.

    1993-01-01

    Detail design of the control system for the West Valley Nuclear Services Vitrification Facility transfer cart has been completed by Oak Ridge National Laboratory. This report documents the requirements and describes the detail design of that equipment and control software. Copies of significant design documents including analysis and testing reports and design drawings are included in the Appendixes.

  7. Acrobatic Drama Pilgrimage to the West Winning Wenhua Award

    Institute of Scientific and Technical Information of China (English)

    王玉

    2007-01-01

    The 8th China Arts Festival was held in Wuhan from 5th to 20th of November.Guangzhou Acrobatic Troupe's acrobatic drama-"Pilgrimage to the West"competed for the Wenhua Award, which is the most prestigious prize for performance art given by the Chinese government.

  8. Fonio (Digitaria exilis) in West Africa: towards improving nutrient quality

    NARCIS (Netherlands)

    Koreissi, Y.

    2015-01-01

    Fonio (Digitaria exilis) in West Africa: Towards improving nutrient quality Abstract Hidden hunger affects a far greater percentage of the world’s population with iron and zinc deficiencies being the most common, particularly affecting women of reproductive ag

  9. Humanistic Traditions, East and West: Convergence and Divergence

    Science.gov (United States)

    Kato, Morimichi

    2016-01-01

    The term "humanism" is Western in origin. It denotes the tradition that places special emphasis on cultivation of letters for education. In the West, this tradition was originated with sophists and Isocrates, established by Cicero, and was developed by Renaissance humanists. East Asia, however, also has its own humanistic traditions with…

  10. Clifford Sifton, Immigration and the Settling of the West.

    Science.gov (United States)

    Bennett, Paul W.

    1985-01-01

    Clifford Sifton stands out in most Canadian history texts as the architect of the immigration policy that settled the prairies West and shaped the development of modern Canada. Background information on Sifton is provided. Letters Sifton wrote regarding immigration policy are analyzed. (RM)

  11. Mixed farming : scope and constraints in West African savanna

    NARCIS (Netherlands)

    Slingerland, M.

    2000-01-01

    Since colonial times the crop livestock integration concept has been a leading development model in francophone West Africa. So far, it has failed in certain aspects, such as cultivation of fodder crops, intensification of cereal production through animal traction, and sedentarisation of

  12. The Dendrochronological Dating of Timber Crossings in West Jutland, Denmark

    DEFF Research Database (Denmark)

    Daly, Aoife

    2006-01-01

    The dendrochronological analyses of a large number of timbers from two sites are described. Nybro causeway in west Jutland, Denmark, which was built in the 8th century AD, consists of numerous phases, identifiable due to the large number of dendrochronologically dated timbers, of which many had...

  13. Raccoon roundworm in raccoons in central West Virginia

    Science.gov (United States)

    Sheldon F. Owen; John W. Edwards; W. Mark Ford; James M. Crum; Petra Bohall. Wood

    2004-01-01

    We investigated the occurrence of raccoon roundworm (Baylisascaris procyonis) in common raccoons (Procyon lotor) in the Allegheny Mountains of West Virginia during spring (n = 9, April-June) and fall (n = 5, August-October) 2001 and spring (n = 1) and fall (n = 4) 2002. We found no evidence of B. procyonis...

  14. West German producers step up enhanced recovery techniques

    Energy Technology Data Exchange (ETDEWEB)

    1978-04-15

    With the lure of more than $12 for every barrel of oil they find, West German oil companies are leaving few rocks unturned in a scramble for additional crude reserves. Most are convinced that in onshore West Germany, at least, the biggest new finds may well be in reservoirs discovered some years back. Reservoir engineers and economists are convinced that the recent large increases in crude prices will make it economical to recover far more oil from wells than ever before. Using only the advanced recovery techniques now available, West German reserves, it is estimated, could more than double. Total original oil in place discovered in West Germany is put at more than 300 million tons, with at least 200 million tons of highly viscous crude located in the Ems area in the northwest corner of the country. Cumulative production to date totals just over 65 million tons; proven recoverable reserves total 81 million tons. This leaves some part to unfavorable economics. However, there are some 45 million breakthrough tons considered by experts to be potentially recoverable subject only to refinement of known recovery technology.

  15. Sinopec Strengthens Exploration and Development in West China

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    @@ Yakela-Dalaoba Gas Field brought under development The first gas field of Sinopec Northwest Company was brought under development in early December, 2004. It is one of the important measures Sinopec has taken to support the campaign for developing West China and promote the local economic development in Xinjiang.

  16. Teaching in West Africa: Dig beneath the Surface

    Science.gov (United States)

    Briam, Carol

    2007-01-01

    In this article, the author shares her experience teaching English to adult learners at the American Cultural Center in Dakar, Senegal, a poor, primarily Muslim country and former French colony in West Africa. Her class was composed of about 30 students, whose age ranged from about 18 to 50. They were mostly men and mostly Senegalese, along with a…

  17. Increase in West Nile neuroinvasive disease after Hurricane Katrina.

    Science.gov (United States)

    Caillouët, Kevin A; Michaels, Sarah R; Xiong, Xu; Foppa, Ivo; Wesson, Dawn M

    2008-05-01

    After Hurricane Katrina, the number of reported cases of West Nile neuroinvasive disease (WNND) sharply increased in the hurricane-affected regions of Louisiana and Mississippi. In 2006, a >2-fold increase in WNND incidence was observed in the hurricane-affected areas than in previous years.

  18. Schooling of Immigrant Children in West Germany, Sweden, England

    Science.gov (United States)

    Willke, I.

    1975-01-01

    The focus of this article is on children of migrant workers and immigrants in the schools of West Germany, Sweden and England. One central problem, that of language, is considered both as it is dealt with in policy, i. e., in curricula, and as it is actually implemented in some programs, which are typical for the actions in these countries.…

  19. Observations during the first K West fuel shipping campaign

    Energy Technology Data Exchange (ETDEWEB)

    Makenas, B.J.

    1995-11-01

    Three fuel elements were shipped to the 300 Area hotcells during the first characterization shipping campaign from K West Basin. This document summarizes observations made during this campaign including the gas, liquid, and sludge content of the observed canisters. Included in an appendix is a detailed evaluation of fuel element condition for each canister opened

  20. The Shallow-water Octocorallia of the West Indian Region

    NARCIS (Netherlands)

    Bayer, Frederick M.

    1961-01-01

    The alcyonarian fauna of the West Indies is prolific and conspicuous and has been known for many years, with the natural result that a great many more species have been described than actually exist. The deep-water fauna, which received little attention prior to the work of VERRILL, was thoroughly