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Sample records for metapneumovirus humano hmpv

  1. Analysis of the genomic sequence of a human metapneumovirus (hMPV).

    NARCIS (Netherlands)

    B.G. van den Hoogen (Bernadette); T.M. Bestebroer (Theo); A.D.M.E. Osterhaus (Albert); R.A.M. Fouchier (Ron)

    2002-01-01

    textabstractWe recently described the isolation of a novel paramyxovirus from children with respiratory tract disease in The Netherlands. Based on biological properties and limited sequence information the virus was provisionally classified as the first nonavian member of the Metapneumovirus genus

  2. Infección por metapneumovirus humano en niños hospitalizados por una enfermedad respiratoria aguda grave: Descripción clínico- epidemiológica A human metapneumovirus infection in hospitalized infant patients with severe acute respiratory tract infection: A clinical and epidemiological view

    OpenAIRE

    JAIME LOZANO C; LETICIA YÁÑEZ P; MICHELANGELO LAPADULA A; MÓNICA LAFOURCADE R; FELIPE BURGOS F; LUIS HERRADA H; ISOLDA BUDNIK O

    2009-01-01

    El metapneumovirus humano (hMPV) es un virus de reciente diagnóstico. Se asocia con infecciones respiratorias agudas altas y bajas (IRAb). Se efectuó un estudio prospectivo durante dos años con el objetivo de evaluar la tasa de circulación y los hallazgos clínicos asociados a la infección por hMPV en niños hospitalizados por una IRAb grave. Resultados: hMPV fue demostrado en 24 (10,5%) de los 229 niños enrolados. 42% de los pacientes con hMPV eran menores de 12 meses de edad y el 58% tenía al...

  3. Avaliação in vitro da atividade antiviral de extratos de plantas frente ao metapneumovirus aviário (AMPV) e vírus respiratório sincicial bovino (BRSV)

    OpenAIRE

    Matheus Cavalheiro Martini

    2010-01-01

    Resumo: Para avaliar a atividade antiviral dos extratos de plantas brasileiras foram eleitos o Metapneumovirus aviário (aMPV) e o vírus Respiratório sincicial bovino (BRSV) pertences à família Paramyxoviridae, subfamília Pneumovirinae, gêneros Metapneumovirus e Pneumovirus respectivamente. Tanto o aMPV quanto o BRSV são vírus semelhantes aos que causam doenças em humanos como o vírus respiratório sincicial humano (HRSV) e metapneumovírus humano (hMPV). O objetivo do presente trabalho foi aval...

  4. Avian and human metapneumovirus.

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    Broor, Shobha; Bharaj, Preeti

    2007-04-01

    Pneumovirus infection remains a significant problem for both human and veterinary medicine. Both avian pneumovirus (aMPV, Turkey rhinotracheitis virus) and human metapneumovirus (hMPV) are pathogens of birds and humans, which are associated with respiratory tract infections. Based on their different genomic organization and low level of nucleotide (nt) and amino acid (aa) identity with paramyxoviruses in the genus Pneumovirus, aMPV and hMPV have been classified into a new genus referred to as Metapneumovirus. The advancement of our understanding of pneumovirus biology and pathogenesis of pneumovirus disease in specific natural hosts can provide us with strategies for vaccine formulations and combined antiviral and immunomodulatory therapies.

  5. hMPV Lineage Nomenclature and Heparin Binding

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    David Gordon

    2013-10-01

    Full Text Available Human metapneumovirus (hMPV, first described in 2001 [1], is responsible for causing serious respiratory illness in young children, the elderly and immunocompromised patients. Four distinct lineages of hMPV have been identified with the original nomenclature for these subgroups (A1, A2, B1 and B2, reported by van den Hoogen et al. [2], utilised by many. An alternate terminology (1A, 1B, 2A and 2B was also published by Ishiguro et al. in 2004 [3] which has been adopted by others. However, this has caused some confusion in the interpretation of publication results as the terminology is similar yet describes different subtypes. As a result, a number of investigators have made a submission to the International Committee on Taxonomy of Viruses (ICTV, ICTV taxonomic proposal 2012.012V for the official adoption of the original terminology as an approved nomenclature for hMPV [4]. We welcome this officially approved nomenclature which should provide clarification of these subtypes in future. Therefore to assist with the interpretation of our recently published research in the 2012 special issue of Viruses: Pneumoviruses and Metapneumoviruses entitled “Diversity in Glycosaminoglycan Binding Amongst hMPV G Protein Lineages” [5] we have updated the Figure 3 in this letter (see Figure 1, showing the proposed ICTV terminology compared to the Ishiguro classification (used in our publication. Note that in the original publication the alphanumeric order for the Ishiguro classification was transposed (e.g., 1A was referred to as A1.

  6. Human Metapneumovirus in Turkey Poults

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    Velayudhan, Binu T.; Nagaraja, Kakambi V.; Thachil, Anil J.; Shaw, Daniel P.; Gray, Gregory C.

    2006-01-01

    This study was conducted to reexamine the hypothesis that human metapneumovirus (hMPV) will not infect turkeys. Six groups of 2-week-old turkeys (20 per group) were inoculated oculonasally with 1 of the following: noninfected cell suspension; hMPV genotype A1, A2, B1, or B2; or avian metapneumovirus (aMPV) subtype C. Poults inoculated with hMPV showed nasal discharge days 4–9 postexposure. Specific viral RNA and antigen were detected by reverse-transcription PCR and immunohistochemical evaluation, respectively, in nasal turbinates of birds exposed to hMPV. Nasal turbinates of hMPV-infected turkeys showed inflammatory changes and mucus accumulation. Each of the 4 hMPV genotypes caused a transient infection in turkeys as evidenced by clinical signs, detection of hMPV in turbinates, and histopathologic examination. Detailed investigation of cross-species pathogenicity of hMPV and aMPV and its importance for human and animal health is needed. PMID:17235379

  7. Specificity and functional interaction of the polymerase complex proteins of human and avian metapneumoviruses

    NARCIS (Netherlands)

    M.T. de Graaf (Marieke); S. Herfst (Sander); E.J.A. Schrauwen (Eefje); Y. Choi (Ying); B.G. van den Hoogen (Bernadette); A.D.M.E. Osterhaus (Albert); R.A.M. Fouchier (Ron)

    2008-01-01

    textabstractHuman metapneumovirus (HMPV) and avian metapneumovirus (AMPV) have a similar genome organization and protein composition, but a different host range. AMPV subgroup C (AMPV-C) is more closely relaled to HMPV than other AMPVs. To investigate the specificity and functional interaction of

  8. Molecular epidemiology and evolution of human respiratory syncytial virus and human metapneumovirus

    NARCIS (Netherlands)

    Gaunt, Eleanor R.; Jansen, Rogier R.; Poovorawan, Yong; Templeton, Kate E.; Toms, Geoffrey L.; Simmonds, Peter

    2011-01-01

    Human respiratory syncytial virus (HRSV) and human metapneumovirus (HMPV) are ubiquitous respiratory pathogens of the Pneumovirinae subfamily of the Paramyxoviridae. Two major surface antigens are expressed by both viruses; the highly conserved fusion (F) protein, and the extremely diverse

  9. Molecular Epidemiology and Evolution of Human Respiratory Syncytial Virus and Human Metapneumovirus

    NARCIS (Netherlands)

    Gaunt, E.R.; Jansen, R.R.; Poovorawan, Y.; Templeton, K.E.; Toms, G.L.; Simmonds, P.

    2011-01-01

    Human respiratory syncytial virus (HRSV) and human metapneumovirus (HMPV) are ubiquitous respiratory pathogens of the Pneumovirinae subfamily of the Paramyxoviridae. Two major surface antigens are expressed by both viruses; the highly conserved fusion (F) protein, and the extremely diverse

  10. Human Respiratory Syncytial Virus and Human Metapneumovirus

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    Luciana Helena Antoniassi da Silva

    2009-08-01

    Full Text Available The human respiratory syncytial virus (hRSV and the human metapneumovírus (hMPV are main etiological agents of acute respiratory infections (ARI. The ARI is an important cause of childhood morbidity and mortality worldwide.  hRSV and hMPV are members of the Paramyxoviridae. They are enveloped, non-segmented viruses, with negative-sense single stranded genomes. Respiratory syncytial virus (hRSV is the best characterized agent viral of this group, associated with respiratory diseases in lower respiratory tract. Recently, a new human pathogen belonging to the subfamily Pneumovirinae was identified, the human metapneumovirus (hMPV, which is structurally similar to the hRSV, in genomic organization, viral structure, antigenicity and clinical symptoms.  The subfamily Pneumovirinae contains two genera: genus Pneumovirus contains hRSV, the bovine (bRSV, as well as the ovine and caprine respiratory syncytial virus and pneumonia virus of mice, the second genus Metapneumovirus, consists of avian metapneumovirus (aMPV and human metapneumovirus (hMPV. In this work, we present a brief narrative review of the literature on important aspects of the biology, epidemiology and clinical manifestations of infections by two respiratory viruses.

  11. Antigenic and genetic variability of human metapneumoviruses

    NARCIS (Netherlands)

    S. Herfst (Sander); L. Sprong; P.A. Cane; E. Forleo-Neto; A.D.M.E. Osterhaus (Albert); R.A.M. Fouchier (Ron); R.L. de Swart (Rik); B.G. van den Hoogen (Bernadette)

    2004-01-01

    textabstractHuman metapneumovirus (HMPV) is a member of the subfamily Pneumovirinae within the family Paramyxo- viridae. Other members of this subfamily, respiratory syncytial virus and avian pneumovirus, can be divided into subgroups on the basis of genetic or antigenic differences or both. For

  12. Fusion protein is the main determinant of metapneumovirus host tropism.

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    de Graaf, Miranda; Schrauwen, Eefje J A; Herfst, Sander; van Amerongen, Geert; Osterhaus, Albert D M E; Fouchier, Ron A M

    2009-06-01

    Human metapneumovirus (HMPV) and avian metapneumovirus subgroup C (AMPV-C) infect humans and birds, respectively. This study confirmed the difference in host range in turkey poults, and analysed the contribution of the individual metapneumovirus genes to host range in an in vitro cell-culture model. Mammalian Vero-118 cells supported replication of both HMPV and AMPV-C in contrast to avian quail fibroblast (QT6) cells in which only AMPV-C replicated to high titres. Inoculation of Vero-118 and QT6 cells with recombinant HMPV in which genes were exchanged with those of AMPV-C revealed that the metapneumovirus fusion (F) protein is the main determinant for host tropism. Chimeric viruses in which polymerase complex proteins were exchanged between HMPV and AMPV-C replicated less efficiently compared with HMPV in QT6 cells. Using mini-genome systems, it was shown that exchanging these polymerase proteins resulted in reduced replication and transcription efficiency in QT6 cells. Examination of infected Vero-118 and QT6 cells revealed that viruses containing the F protein of AMPV-C yielded larger syncytia compared with viruses containing the HMPV F protein. Cell-content mixing assays revealed that the F protein of AMPV-C was more fusogenic compared with the F protein of HMPV, and that the F2 region is responsible for the difference observed between AMPV-C and HMPV F-promoted fusion in QT6 and Vero-118 cells. This study provides insight into the determinants of host tropism and membrane fusion of metapneumoviruses.

  13. Absence of human metapneumovirus co-infection in cases of severe respiratory syncytial virus infection

    NARCIS (Netherlands)

    van Woensel, J. B. M.; Bos, A. P.; Lutter, R.; Rossen, J. W. A.; Schuurman, R.

    2006-01-01

    It has been suggested that co-infection of human metapneumovirus (hMPV) in severe respiratory syncytial (RSV) virus bronchiolitis is very common. To evaluate the epidemiology of hMPV co-infection in children with severe lower respiratory tract infection caused by RSV virus. This was an observational

  14. Absence of human metapneumovirus co-infection in cases of severe respiratory syncytial virus infection

    NARCIS (Netherlands)

    van Woensel, J B M; Bos, A P; Lutter, R; Rossen, J W A; Schuurman, R

    It has been suggested that co-infection of human metapneumovirus (hMPV) in severe respiratory syncytial (RSV) virus bronchiolitis is very common. To evaluate the epidemiology of hMPV co-infection in children with severe lower respiratory tract infection caused by RSV virus. This was an observational

  15. Human metapneumovirus and respiratory syncytial virus in hospitalized danish children with acute respiratory tract infection

    DEFF Research Database (Denmark)

    von Linstow, Marie-Louise; Henrik Larsen, Hans; Koch, Anders

    2004-01-01

    The newly discovered human metapneumovirus (hMPV) has been shown to be associated with respiratory illness. We determined the frequencies and clinical features of hMPV and respiratory syncytial virus (RSV) infections in 374 Danish children with 383 episodes of acute respiratory tract infection...... children 1-6 months of age. Asthmatic bronchitis was diagnosed in 66.7% of hMPV and 10.6% of RSV-infected children (p respiratory support. hMPV is present in young...

  16. Human Metapneumovirus Antagonism of Innate Immune Responses

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    Xiaoyong Bao

    2012-12-01

    Full Text Available  Human metapneumovirus (hMPV is a recently identified RNA virus belonging to the Paramyxoviridae family, which includes several major human and animal pathogens. Epidemiological studies indicate that hMPV is a significant human respiratory pathogen with worldwide distribution. It is associated with respiratory illnesses in children, adults, and immunocompromised patients, ranging from upper respiratory tract infections to severe bronchiolitis and pneumonia. Interferon (IFN represents a major line of defense against virus infection, and in response, viruses have evolved countermeasures to inhibit IFN production as well as IFN signaling. Although the strategies of IFN evasion are similar, the specific mechanisms by which paramyxoviruses inhibit IFN responses are quite diverse. In this review, we will present an overview of the strategies that hMPV uses to subvert cellular signaling in airway epithelial cells, the major target of infection, as well as in primary immune cells.

  17. Human metapneumovirus antagonism of innate immune responses.

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    Kolli, Deepthi; Bao, Xiaoyong; Casola, Antonella

    2012-12-07

     Human metapneumovirus (hMPV) is a recently identified RNA virus belonging to the Paramyxoviridae family, which includes several major human and animal pathogens. Epidemiological studies indicate that hMPV is a significant human respiratory pathogen with worldwide distribution. It is associated with respiratory illnesses in children, adults, and immunocompromised patients, ranging from upper respiratory tract infections to severe bronchiolitis and pneumonia. Interferon (IFN) represents a major line of defense against virus infection, and in response, viruses have evolved countermeasures to inhibit IFN production as well as IFN signaling. Although the strategies of IFN evasion are similar, the specific mechanisms by which paramyxoviruses inhibit IFN responses are quite diverse. In this review, we will present an overview of the strategies that hMPV uses to subvert cellular signaling in airway epithelial cells, the major target of infection, as well as in primary immune cells.

  18. Chest radiographic features of human metapneumovirus infection in pediatric patients

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    Hilmes, Melissa A.; Daniel Dunnavant, F.; Singh, Sudha P.; Ellis, Wendy D. [Vanderbilt University School of Medicine, Department of Radiology, Nashville, TN (United States); Payne, Daniel C. [Centers for Disease Control and Prevention, Atlanta, GA (United States); Zhu, Yuwei [Vanderbilt University School of Medicine, Department of Biostatistics, Nashville, TN (United States); Griffin, Marie R. [Vanderbilt University School of Medicine, Department of Health Policy, Nashville, TN (United States); Edwards, Kathryn M. [Vanderbilt University School of Medicine, Department of Pediatrics, Nashville, TN (United States); Williams, John V. [University of Pittsburgh School of Medicine, Department of Pediatrics, Pittsburgh, PA (United States); University of Pittsburgh of UPMC, Children' s Hospital of Pittsburgh, Pittsburgh, PA (United States)

    2017-12-15

    Human metapneumovirus (HMPV) was identified in 2001 and is a common cause of acute respiratory illness in young children. The radiologic characteristics of laboratory-confirmed HMPV acute respiratory illness in young children have not been systematically assessed. We systematically evaluated the radiographic characteristics of acute respiratory illness associated with HMPV in a prospective cohort of pediatric patients. We included chest radiographs from children <5 years old with acute respiratory illness who were enrolled in the prospective New Vaccine Surveillance Network (NVSN) study from 2003 to 2009 and were diagnosed with HMPV by reverse transcription-polymerase chain reaction (RT-PCR). Of 215 HMPV-positive subjects enrolled at our tertiary care children's hospital, 68 had chest radiographs obtained by the treating clinician that were available for review. Two fellowship-trained pediatric radiologists, independently and then in consensus, retrospectively evaluated these chest radiographs for their radiographic features. Parahilar opacities were the most commonly observed abnormality, occurring in 87% of children with HMPV. Hyperinflation also occurred frequently (69%). Atelectasis (40%) and consolidation (18%) appeared less frequently. Pleural effusion and pneumothorax were not seen on any radiographs. The clinical presentations of HMPV include bronchiolitis, croup and pneumonia. Dominant chest radiographic abnormalities include parahilar opacities and hyperinflation, with occasional consolidation. Recognition of the imaging patterns seen with common viral illnesses like respiratory syncytial virus (RSV) and HMPV might facilitate diagnosis and limit unnecessary antibiotic treatment. (orig.)

  19. Chest radiographic features of human metapneumovirus infection in pediatric patients

    International Nuclear Information System (INIS)

    Hilmes, Melissa A.; Daniel Dunnavant, F.; Singh, Sudha P.; Ellis, Wendy D.; Payne, Daniel C.; Zhu, Yuwei; Griffin, Marie R.; Edwards, Kathryn M.; Williams, John V.

    2017-01-01

    Human metapneumovirus (HMPV) was identified in 2001 and is a common cause of acute respiratory illness in young children. The radiologic characteristics of laboratory-confirmed HMPV acute respiratory illness in young children have not been systematically assessed. We systematically evaluated the radiographic characteristics of acute respiratory illness associated with HMPV in a prospective cohort of pediatric patients. We included chest radiographs from children <5 years old with acute respiratory illness who were enrolled in the prospective New Vaccine Surveillance Network (NVSN) study from 2003 to 2009 and were diagnosed with HMPV by reverse transcription-polymerase chain reaction (RT-PCR). Of 215 HMPV-positive subjects enrolled at our tertiary care children's hospital, 68 had chest radiographs obtained by the treating clinician that were available for review. Two fellowship-trained pediatric radiologists, independently and then in consensus, retrospectively evaluated these chest radiographs for their radiographic features. Parahilar opacities were the most commonly observed abnormality, occurring in 87% of children with HMPV. Hyperinflation also occurred frequently (69%). Atelectasis (40%) and consolidation (18%) appeared less frequently. Pleural effusion and pneumothorax were not seen on any radiographs. The clinical presentations of HMPV include bronchiolitis, croup and pneumonia. Dominant chest radiographic abnormalities include parahilar opacities and hyperinflation, with occasional consolidation. Recognition of the imaging patterns seen with common viral illnesses like respiratory syncytial virus (RSV) and HMPV might facilitate diagnosis and limit unnecessary antibiotic treatment. (orig.)

  20. Human Metapneumovirus Infection in Jordanian Children: Epidemiology and Risk Factors for Severe Disease

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    Schuster, Jennifer E.; Khuri-Bulos, Najwa; Faouri, Samir; Shehabi, Asem; Johnson, Monika; Wang, Li; Fonnesbeck, Christopher; Williams, John V.; Halasa, Natasha

    2016-01-01

    Background Human metapneumovirus (HMPV) is a leading cause of acute respiratory tract infection (ARTI) in young children. Our objectives were to define HMPV epidemiology and circulating strains and determine markers of severe disease in Jordanian children. Methods We conducted a prospective study March 16, 2010-March 31, 2013 using quantitative RT-PCR to determine the frequency of HMPV infection among children <2 years old admitted with fever and/or acute respiratory illness to a major government hospital in Amman, Jordan. Results HMPV was present in 273/3168 (8.6%) of children presenting with ARTI. HMPV A2, B1, and B2, but not A1, were detected during the 3-year period. HMPV-infected children were older and more likely to be diagnosed with bronchopneumonia than HMPV-negative children. HMPV-infected children with lower respiratory tract infection (LRTI) had higher rates of cough and shortness of breath than children with LRTI infected with other or no identifiable viruses. Symptoms and severity were not different between children with HMPV only compared with HMPV co-infection. Children with HMPV subgroup A infection were more likely to require supplemental oxygen. In a multivariate analysis, HMPV subgroup A and age <6 months were independently associated with supplemental oxygen requirement. Conclusions HMPV is a leading cause of acute respiratory tract disease in Jordanian children <2 years old. HMPV A and young age were associated with severe disease. Ninety percent of HMPV-infected hospitalized children were full-term and otherwise healthy, in contrast to high-income nations; thus, factors contributing to disease severity likely vary depending on geographic and resource differences. PMID:26372450

  1. hMPV and RSV: subtle differences but comparable severity

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    Nour Akhras

    2010-08-01

    Full Text Available

     Human metapneumovirus (hMPV is a recently discovered virus that causes respiratory illness in children and can lead to hospitalization.   This study was undertaken to

  2. An outbreak of severe respiratory tract infection caused by human metapneumovirus in a residential care facility for elderly in Utrecht, the Netherlands, January to March 2010

    NARCIS (Netherlands)

    M.J.T. Wierik (M J Te); D.T. Nguyen (Tien); M.F.C. Beersma (Thijs); S.F. Thijsen (Steven); K.A. Heemstra

    2012-01-01

    textabstractRecognition of infections with human metapneumovirus (HMPV) among institutionalised elderly is rising. When HMPV was found to be the causative agent of an outbreak of pneumonia in a residential care facility for elderly in the Netherlands, an elaborate outbreak investigation was set up,

  3. Limited inter- and intra-patient sequence diversity of the genetic lineage a human metapneumovirus fusion gene

    DEFF Research Database (Denmark)

    Winther, T.N.; Madsen, C.D.; Pedersen, Anders Gorm

    2005-01-01

    Human metapneumovirus (hMPV) is associated with respiratory tract illness especially in young children. Two hMPV genetic lineages, A and B, and four sublineages A1, A2 and B1, B2 have been defined. Infection with hMPV occurs through membrane fusion mediated by the hMPV fusion (F) protein...... been infected with at least two viruses. Several independent viruses contained premature stop codons in exactly identical positions resulting in truncated fusion proteins. Possibly this is a mechanism for immune system evasion. The F protein is a major antigenic determinant, and the limited sequence...

  4. Small Animal Models for Human Metapneumovirus: Cotton Rat is More Permissive than Hamster and Mouse

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    Zhang, Yu; Niewiesk, Stefan; Li, Jianrong

    2014-01-01

    Human metapneumovirus (hMPV) is the second most prevalent causative agent of pediatric respiratory infections worldwide. Currently, there are no vaccines or antiviral drugs against this virus. One of the major hurdles in hMPV research is the difficulty to identify a robust small animal model to accurately evaluate the efficacy and safety of vaccines and therapeutics. In this study, we compared the replication and pathogenesis of hMPV in BALB/c mice, Syrian golden hamsters, and cotton rats. It was found that BALB/c mice are not permissive for hMPV infection despite the use of a high dose (6.5 log10 PFU) of virus for intranasal inoculation. In hamsters, hMPV replicated efficiently in nasal turbinates but demonstrated only limited replication in lungs. In cotton rats, hMPV replicated efficiently in both nasal turbinate and lung when intranasally administered with three different doses (4, 5, and 6 log10 PFU) of hMPV. Lungs of cotton rats infected by hMPV developed interstitial pneumonia with mononuclear cells infiltrates and increased lumen exudation. By immunohistochemistry, viral antigens were detected at the luminal surfaces of the bronchial epithelial cells in lungs. Vaccination of cotton rats with hMPV completely protected upper and lower respiratory tract from wildtype challenge. The immunization also elicited elevated serum neutralizing antibody. Collectively, these results demonstrated that cotton rat is a robust small animal model for hMPV infection. PMID:25438015

  5. Molecular Analysis of Human Metapneumovirus Detected in Patients with Lower Respiratory Tract Infection in Upper Egypt

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    Embarek Mohamed, Mona S.; Jacobsen, Sonja; Thabit, Amany G.; Badary, Mohamed S.; Brune, Wolfram; Schweiger, Brunhilde; Osmann, Ahmed H.

    2014-01-01

    Introduction. Since 2001, when Human metapneumovirus (HMPV) was isolated in the Netherlands, the virus has been detected in several continents. Although reports have confirmed the prevalence of HMPV worldwide, data from Egypt remain limited. HMPV plays an important role in respiratory tract infections in individuals of all ages particularly in children. This study was aimed at estimating the prevalence of HMPV in patients with community-acquired lower respiratory infection in Upper Egypt and characterizing the circulating Egyptian HMPV strains for the first time. Materials and Methods. From 2005 to 2008, respiratory samples from 520 patients were analyzed for the presence of HMPV by real-time RT-PCR. Molecular and phylogenetic analyses were performed on partial fusion gene sequences of HMPV-positive patients. Results. HMPV-positive patients were detected in 2007-2008. The overall infection rate was 4%, while 57% of the patients were children. Sequence analysis demonstrated circulation of subgroup B viruses with predominance of lineage B2. Nucleotide sequence identity within lineage B1 was 98.8%–99.7% and higher than that in lineage B2 (94.3%–100%). Three new amino acid substitutions (T223N, R229K, and D280N) of lineage B2 were observed. Conclusion. HMPV is a major viral pathogen in the Egyptian population especially in children. During 2007-2008, predominantly HMPV B2 circulated in Upper Egypt. PMID:24669221

  6. Performance of direct immunofluorescence assay for the detection of human metapneumovirus under clinical laboratory settings.

    Science.gov (United States)

    Wolf, Jonas Michel; Gregianini, Tatiana Schäffer; Seadi, Claudete Maria Farina; Tumioto, Gabriela Luchiari; Dambrós, Bibiana Paula; Lehmann, Fernanda Kieling Moreira; Carli, Silvia De; Ikuta, Nilo; Lunge, Vagner Ricardo

    2015-01-01

    Human metapneumovirus (hMPV) is an emergent human respiratory pathogen. This study aimed to evaluate the performance of direct immunofluorescence (DIF) to detect hMPV in a clinical laboratory setting. Nasopharyngeal aspirate samples (448) of children and adults with respiratory illness were used to detect hMPV by using DIF and real time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assays. In all, 36 (8%) samples were positive by DIF and 94 (21%) were positive by qRT-PCR. Direct immunofluorescence specificity was 99% and sensitivity was 38%. DIF is not very sensitive under clinical laboratory settings.

  7. Adult human metapneumovirus encephalitis: A case report highlighting challenges in clinical management and functional outcome.

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    Tan, Y L; Wee, T C

    2017-12-01

    We report a rare case of adult human metapneumovirus (HMPV) in a healthy 32-year-old man. There was dramatic deterioration in his condition developing pneumonia with Type-I respiratory failure and encephalitis. He needed mechanical ventilation in the intensive care setting and was treated with intravenous ribavirin. Post-extubation he remained severely physically and cognitively impaired despite rehabilitation. Treatment of HMPV pneumonia is at present, still without specific antiviral therapy. Managing HMPV-encephalitis remained supportive and challenging. More definite treatment strategies are needed.

  8. Diagnosis of human metapneumovirus infection in immunosuppressed lung transplant recipients and children evaluated for pertussis.

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    Dare, Ryan; Sanghavi, Sonali; Bullotta, Arlene; Keightley, Maria-Cristina; George, Kirsten St; Wadowsky, Robert M; Paterson, David L; McCurry, Kenneth R; Reinhart, Todd A; Husain, Shahid; Rinaldo, Charles R

    2007-02-01

    Human metapneumovirus (hMPV) is a recently discovered paramyxovirus that is known to cause respiratory tract infections in children and immunocompromised individuals. Given the difficulties of identifying hMPV by conventional culture, molecular techniques could improve the detection of this virus in clinical specimens. In this study, we developed a real-time reverse transcription-PCR (RT-PCR) assay designed to detect the four genetic lineages of hMPV. This assay and a commercial real-time nucleic acid sequence-based amplification (NASBA) assay (bioMérieux, Durham, NC) were used to determine the prevalence of hMPV in 114 immunosuppressed asymptomatic and symptomatic lung transplant recipients and 232 pediatric patients who were being evaluated for pertussis. hMPV was detected in 4.3% of the immunosuppressed lung transplant recipients and in 9.9% of children evaluated for pertussis. Both RT-PCR and NASBA assays were efficient in detection of hMPV infection in respiratory specimens. Even though hMPV was detected in a small number of the lung transplant recipients, it was still the most prevalent etiologic agent detected in patients with respiratory symptoms. In both of these diverse patient populations, hMPV infection was the most frequent viral respiratory tract infection identified. Given our findings, infection with hMPV infection should be determined as part of the differential diagnosis of respiratory illnesses.

  9. Diagnosis of Human Metapneumovirus Infection in Immunosuppressed Lung Transplant Recipients and Children Evaluated for Pertussis▿

    Science.gov (United States)

    Dare, Ryan; Sanghavi, Sonali; Bullotta, Arlene; Keightley, Maria-Cristina; George, Kirsten St.; Wadowsky, Robert M.; Paterson, David L.; McCurry, Kenneth R.; Reinhart, Todd A.; Husain, Shahid; Rinaldo, Charles R.

    2007-01-01

    Human metapneumovirus (hMPV) is a recently discovered paramyxovirus that is known to cause respiratory tract infections in children and immunocompromised individuals. Given the difficulties of identifying hMPV by conventional culture, molecular techniques could improve the detection of this virus in clinical specimens. In this study, we developed a real-time reverse transcription-PCR (RT-PCR) assay designed to detect the four genetic lineages of hMPV. This assay and a commercial real-time nucleic acid sequence-based amplification (NASBA) assay (bioMérieux, Durham, NC) were used to determine the prevalence of hMPV in 114 immunosuppressed asymptomatic and symptomatic lung transplant recipients and 232 pediatric patients who were being evaluated for pertussis. hMPV was detected in 4.3% of the immunosuppressed lung transplant recipients and in 9.9% of children evaluated for pertussis. Both RT-PCR and NASBA assays were efficient in detection of hMPV infection in respiratory specimens. Even though hMPV was detected in a small number of the lung transplant recipients, it was still the most prevalent etiologic agent detected in patients with respiratory symptoms. In both of these diverse patient populations, hMPV infection was the most frequent viral respiratory tract infection identified. Given our findings, infection with hMPV infection should be determined as part of the differential diagnosis of respiratory illnesses. PMID:17065270

  10. Human metapneumovirus - what we know now [version 1; referees: 2 approved

    Directory of Open Access Journals (Sweden)

    Nazly Shafagati

    2018-02-01

    Full Text Available Human metapneumovirus (HMPV is a leading cause of acute respiratory infection, particularly in children, immunocompromised patients, and the elderly. HMPV, which is closely related to avian metapneumovirus subtype C, has circulated for at least 65 years, and nearly every child will be infected with HMPV by the age of 5. However, immunity is incomplete, and re-infections occur throughout adult life. Symptoms are similar to those of other respiratory viral infections, ranging from mild (cough, rhinorrhea, and fever to more severe (bronchiolitis and pneumonia. The preferred method for diagnosis is reverse transcription-polymerase chain reaction as HMPV is difficult to culture. Although there have been many advances made in the past 16 years since its discovery, there are still no US Food and Drug Administration-approved antivirals or vaccines available to treat HMPV. Both small animal and non-human primate models have been established for the study of HMPV. This review will focus on the epidemiology, transmission, and clinical manifestations in humans as well as the animal models of HMPV pathogenesis and host immune response.

  11. Human Metapneumovirus: Insights from a Ten-Year Molecular and Epidemiological Analysis in Germany

    Science.gov (United States)

    Reiche, Janine; Jacobsen, Sonja; Neubauer, Katrin; Hafemann, Susi; Nitsche, Andreas; Milde, Jeanette; Wolff, Thorsten; Schweiger, Brunhilde

    2014-01-01

    Human metapneumovirus (HMPV) is a cause of respiratory tract illness at all ages. In this study the epidemiological and molecular diversity among patients of different ages was investigated. Between 2000–2001 and 2009–2010, HMPV was detected in 3% (138/4,549) of samples from outpatients with influenza-like illness with a new, sensitive real-time RT-PCR assay. Several hundred (797) clinical specimens from hospitalized children below the age of 4 years with acute respiratory illness were investigated and HMPV was detected in 11.9% of them. Investigation of outpatients revealed that HMPV infections occurred in individuals of all ages but were most prevalent in children (0–4 years) and the elderly (>60 years). The most present clinical features of HMPV infections were cough, bronchitis, fever/shivers and pneumonia. About two thirds of HMPV-positive samples were detected in February and March throughout the study period. Molecular characterization of HMPV revealed a complex cyclic pattern of group dominance where HMPV subgroup A and B viruses predominated in general for three consecutive seasons. German HMPV represented all genetic lineages including A1, A2, B1, B2, sub-clusters A2a and A2b. For Germany, not only time-dependent circulation of lineages and sub-clusters was observed but also co-circulation of two or three predominant lineages. Two newly emerging amino acid substitutions (positions 223 and 280) of lineage B2 were detected in seven German HMPV sequences. Our study gives new insights into the molecular epidemiology of HMPV in in- and outpatients over a time period of 10 years for the first time. It is one of only few long-term surveillance studies in Europe, and allows comparative molecular analyses of HMPV circulating worldwide. PMID:24505479

  12. Seroepidemiological study of human metapneumovirus in New Delhi, India

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    S Banerjee

    2011-01-01

    Full Text Available Purpose: There are a few seroepidemiological studies reported on human metapneumovirus (hMPV as hMPV was only discovered in the year 2001. This respiratory virus has been reported to be ubiquitous and associated with respiratory tract infections in all age groups. The present study aimed at determining the prevalence of antibodies to hMPV in children and adults of 1 month to 55 years of age. Materials and Methods: Serum samples from 100 study subjects were tested for hMPV antibody by an in-house ELISA system that used hMPV-infected cell lysate antigen. Result: The prevalence of antibody to hMPV was lowest in children less than 5 years of age (60% and increased throughout age to > 80%. Similarly, geometric mean titres were 1:180 in children less than 5 years of age and reached a peak of 1:419 in adults over 35 years of age. Conclusion: The results show that hMPV infection is acquired early in life and re-infection in later life may maintain the seroprevalence and antibody levels in adult population.

  13. Individual contributions of the human metapneumovirus F, G, and SH surface glycoproteins to the induction of neutralizing antibodies and protective immunity

    International Nuclear Information System (INIS)

    Skiadopoulos, Mario H.; Biacchesi, Stephane; Buchholz, Ursula J.; Amaro-Carambot, Emerito; Surman, Sonja R.; Collins, Peter L.; Murphy, Brian R.

    2006-01-01

    We evaluated the individual contributions of the three surface glycoproteins of human metapneumovirus (HMPV), namely the fusion F, attachment G, and small hydrophobic SH proteins, to the induction of serum HMPV-binding antibodies, serum HMPV-neutralizing antibodies, and protective immunity. Using reverse genetics, each HMPV protein was expressed individually from an added gene in recombinant human parainfluenza virus type 1 (rHPIV1) and used to infect hamsters once or twice by the intranasal route. The F protein was highly immunogenic and protective, whereas G and SH were only weakly or negligibly immunogenic and protective, respectively. Thus, in contrast to other paramyxoviruses, the HMPV attachment G protein is not a major neutralization or protective antigen. Also, although the SH protein of HMPV is a virion protein that is much larger than its counterparts in previously studied paramyxoviruses, it does not appear to be a significant neutralization or protective antigen

  14. Molecular comparisons of full length metapneumovirus (MPV genomes, including newly determined French AMPV-C and -D isolates, further supports possible subclassification within the MPV Genus.

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    Paul A Brown

    Full Text Available Four avian metapneumovirus (AMPV subgroups (A-D have been reported previously based on genetic and antigenic differences. However, until now full length sequences of the only known isolates of European subgroup C and subgroup D viruses (duck and turkey origin, respectively have been unavailable. These full length sequences were determined and compared with other full length AMPV and human metapneumoviruses (HMPV sequences reported previously, using phylogenetics, comparisons of nucleic and amino acid sequences and study of codon usage bias. Results confirmed that subgroup C viruses were more closely related to HMPV than they were to the other AMPV subgroups in the study. This was consistent with previous findings using partial genome sequences. Closer relationships between AMPV-A, B and D were also evident throughout the majority of results. Three metapneumovirus "clusters" HMPV, AMPV-C and AMPV-A, B and D were further supported by codon bias and phylogenetics. The data presented here together with those of previous studies describing antigenic relationships also between AMPV-A, B and D and between AMPV-C and HMPV may call for a subclassification of metapneumoviruses similar to that used for avian paramyxoviruses, grouping AMPV-A, B and D as type I metapneumoviruses and AMPV-C and HMPV as type II.

  15. Molecular Comparisons of Full Length Metapneumovirus (MPV) Genomes, Including Newly Determined French AMPV-C and –D Isolates, Further Supports Possible Subclassification within the MPV Genus

    Science.gov (United States)

    Brown, Paul A.; Lemaitre, Evelyne; Briand, François-Xavier; Courtillon, Céline; Guionie, Olivier; Allée, Chantal; Toquin, Didier; Bayon-Auboyer, Marie-Hélène; Jestin, Véronique; Eterradossi, Nicolas

    2014-01-01

    Four avian metapneumovirus (AMPV) subgroups (A–D) have been reported previously based on genetic and antigenic differences. However, until now full length sequences of the only known isolates of European subgroup C and subgroup D viruses (duck and turkey origin, respectively) have been unavailable. These full length sequences were determined and compared with other full length AMPV and human metapneumoviruses (HMPV) sequences reported previously, using phylogenetics, comparisons of nucleic and amino acid sequences and study of codon usage bias. Results confirmed that subgroup C viruses were more closely related to HMPV than they were to the other AMPV subgroups in the study. This was consistent with previous findings using partial genome sequences. Closer relationships between AMPV-A, B and D were also evident throughout the majority of results. Three metapneumovirus “clusters” HMPV, AMPV-C and AMPV-A, B and D were further supported by codon bias and phylogenetics. The data presented here together with those of previous studies describing antigenic relationships also between AMPV-A, B and D and between AMPV-C and HMPV may call for a subclassification of metapneumoviruses similar to that used for avian paramyxoviruses, grouping AMPV-A, B and D as type I metapneumoviruses and AMPV-C and HMPV as type II. PMID:25036224

  16. Genetic diversity and evolution of human metapneumovirus fusion protein over twenty years

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    Liem Alexis

    2009-09-01

    Full Text Available Abstract Background Human metapneumovirus (HMPV is an important cause of acute respiratory illness in children. We examined the diversity and molecular evolution of HMPV using 85 full-length F (fusion gene sequences collected over a 20-year period. Results The F gene sequences fell into two major groups, each with two subgroups, which exhibited a mean of 96% identity by predicted amino acid sequences. Amino acid identity within and between subgroups was higher than nucleotide identity, suggesting structural or functional constraints on F protein diversity. There was minimal progressive drift over time, and the genetic lineages were stable over the 20-year period. Several canonical amino acid differences discriminated between major subgroups, and polymorphic variations tended to cluster in discrete regions. The estimated rate of mutation was 7.12 × 10-4 substitutions/site/year and the estimated time to most recent common HMPV ancestor was 97 years (95% likelihood range 66-194 years. Analysis suggested that HMPV diverged from avian metapneumovirus type C (AMPV-C 269 years ago (95% likelihood range 106-382 years. Conclusion HMPV F protein remains conserved over decades. HMPV appears to have diverged from AMPV-C fairly recently.

  17. Genetic diversity and evolution of human metapneumovirus fusion protein over twenty years

    Science.gov (United States)

    Yang, Chin-Fen; Wang, Chiaoyin K; Tollefson, Sharon J; Piyaratna, Rohith; Lintao, Linda D; Chu, Marla; Liem, Alexis; Mark, Mary; Spaete, Richard R; Crowe, James E; Williams, John V

    2009-01-01

    Background Human metapneumovirus (HMPV) is an important cause of acute respiratory illness in children. We examined the diversity and molecular evolution of HMPV using 85 full-length F (fusion) gene sequences collected over a 20-year period. Results The F gene sequences fell into two major groups, each with two subgroups, which exhibited a mean of 96% identity by predicted amino acid sequences. Amino acid identity within and between subgroups was higher than nucleotide identity, suggesting structural or functional constraints on F protein diversity. There was minimal progressive drift over time, and the genetic lineages were stable over the 20-year period. Several canonical amino acid differences discriminated between major subgroups, and polymorphic variations tended to cluster in discrete regions. The estimated rate of mutation was 7.12 × 10-4 substitutions/site/year and the estimated time to most recent common HMPV ancestor was 97 years (95% likelihood range 66-194 years). Analysis suggested that HMPV diverged from avian metapneumovirus type C (AMPV-C) 269 years ago (95% likelihood range 106-382 years). Conclusion HMPV F protein remains conserved over decades. HMPV appears to have diverged from AMPV-C fairly recently. PMID:19740442

  18. Chimeric Recombinant Human Metapneumoviruses with the Nucleoprotein or Phosphoprotein Open Reading Frame Replaced by That of Avian Metapneumovirus Exhibit Improved Growth In Vitro and Attenuation In Vivo

    Science.gov (United States)

    Pham, Quynh N.; Biacchesi, Stéphane; Skiadopoulos, Mario H.; Murphy, Brian R.; Collins, Peter L.; Buchholz, Ursula J.

    2005-01-01

    Chimeric versions of recombinant human metapneumovirus (HMPV) were generated by replacing the nucleoprotein (N) or phosphoprotein (P) open reading frame with its counterpart from the closely related avian metapneumovirus (AMPV) subgroup C. In Vero cells, AMPV replicated to an approximately 100-fold-higher titer than HMPV. Surprisingly, the N and P chimeric viruses replicated to a peak titer that was 11- and 25-fold higher, respectively, than that of parental HMPV. The basis for this effect is not known but was not due to obvious changes in the efficiency of gene expression. AMPV and the N and P chimeras were evaluated for replication, immunogenicity, and protective efficacy in hamsters. AMPV was attenuated compared to HMPV in this mammalian host on day 5 postinfection, but not on day 3, and only in the nasal turbinates. In contrast, the N and P chimeras were reduced approximately 100-fold in both the upper and lower respiratory tract on day 3 postinfection, although there was little difference by day 5. The N and P chimeras induced a high level of neutralizing serum antibodies and protective efficacy against HMPV; AMPV was only weakly immunogenic and protective against HMPV challenge, reflecting antigenic differences. In African green monkeys immunized intranasally and intratracheally, the mean peak titer of the P chimera was reduced 100- and 1,000-fold in the upper and lower respiratory tracts, whereas the N chimera was reduced only 10-fold in the lower respiratory tract. Both chimeras were comparable to wild-type HMPV in immunogenicity and protective efficacy. Thus, the P chimera is a promising live HMPV vaccine candidate that paradoxically combines improved growth in vitro with attenuation in vivo. PMID:16306583

  19. Metapneumovirus infections

    Science.gov (United States)

    Avian metapneumovirus (aMPV) causes turkey rhinotracheitis (TRT), an acute upper respiratory tract infection of turkeys, and is also associated with swollen head syndrome (SHS) in chickens and egg production losses in layers. Since the first TRT reported in the late 1970s in South Africa, the virus...

  20. Avian Metapneumoviruses

    Science.gov (United States)

    Avian metapneumovirus (aMPV) is an economically important virus that is the primary causal agent of turkey rhinotracheitis (TRT), also known as avian rhinotracheitis (ART). The virus causes an acute highly contagious infection of the upper respiratory tract in turkeys and was first isolated from tur...

  1. Airway epithelial cell response to human metapneumovirus infection

    International Nuclear Information System (INIS)

    Bao, X.; Liu, T.; Spetch, L.; Kolli, D.; Garofalo, R.P.; Casola, A.

    2007-01-01

    Human metapneumovirus (hMPV) is a major cause of lower respiratory tract infections (LRTIs) in infants, elderly and immunocompromised patients. In this study, we show that hMPV can infect in a similar manner epithelial cells representative of different tracts of the airways. hMPV-induced expression of chemokines IL-8 and RANTES in primary small alveolar epithelial cells (SAE) and in a human alveolar type II-like epithelial cell line (A549) was similar, suggesting that A549 cells can be used as a model to study lower airway epithelial cell responses to hMPV infection. A549 secreted a variety of CXC and CC chemokines, cytokines and type I interferons, following hMPV infection. hMPV was also a strong inducer of transcription factors belonging to nuclear factor (NF)-κB, interferon regulatory factors (IRFs) and signal transducers and activators of transcription (STATs) families, which are known to orchestrate the expression of inflammatory and immunomodulatory mediators

  2. Human Metapneumovirus Infection in Chronic Obstructive Pulmonary Disease: Impact of Glucocorticosteroids and Interferon.

    Science.gov (United States)

    Kan-O, Keiko; Ramirez, Ruben; MacDonald, Martin I; Rolph, Michael; Rudd, Penny A; Spann, Kirsten M; Mahalingam, Suresh; Bardin, Philip G; Thomas, Belinda J

    2017-05-15

    Human metapneumovirus (hMPV) infection is implicated in exacerbations of asthma and chronic obstructive pulmonary disease (COPD). Research into the pathogenesis of infection is restricted to animal models, and information about hMPV replication and inflammatory and immune responses in human disease is limited. Human primary bronchial epithelial cells (PBECs) from healthy and asthmatic subjects and those with COPD were infected with hMPV, with or without glucocorticosteroid (GCS) exposure. Viral replication, inflammatory and immune responses, and apoptosis were analyzed. We also determined whether adjuvant interferon (IFN) can blunt hMPV infection in vitro and in a murine model. hMPV infected human PBECs and viral replication was enhanced in cells from patients with COPD. The virus induced gene expression of IFN-stimulated gene 56 (ISG56) and IFN-β, as well as IFN-γ-inducible protein 10 (IP-10) and regulated on activation, normal T cell expressed and secreted (RANTES), and more so in cells from patients with COPD. GCS exposure enhanced hMPV replication despite increased IFN expression. Augmented virus replication associated with GCS was mediated by reduced apoptosis via induction of antiapoptotic genes. Adjuvant IFN treatment suppressed hMPV replication in PBECs and reduced hMPV viral titers and inflammation in vivo. hMPV infects human PBECs, eliciting innate and inflammatory responses. Replication is enhanced by GCS and adjuvant IFN is an effective treatment, restricting virus replication and proinflammatory consequences of hMPV infections. © The Author 2017. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail journals.permissions@oup.com.

  3. Enhanced lung disease and Th2 response following human metapneumovirus infection in mice immunized with the inactivated virus.

    Science.gov (United States)

    Hamelin, Marie-Eve; Couture, Christian; Sackett, Melanie K; Boivin, Guy

    2007-12-01

    Human metapneumovirus (hMPV) is a paramyxovirus that causes acute respiratory-tract infections in humans. The histopathological and immunological responses to hMPV infection in BALB/c mice immunized with inactivated hMPV were characterized. Animals were immunized intraperitoneally with PBS, supernatant from non-infected LLC-MK2 cells and from heat-inactivated influenza A- or hMPV-infected cells, all in incomplete Freund's adjuvant, or with heat-inactivated hMPV without adjuvant, and then infected intranasally with 10(8) TCID50 virus. Following infection, lung samples and bronchoalveolar lavages were collected for determination of viral titre and cytokine levels and for histopathological studies. On day 1, 26 % of mice immunized with inactivated hMPV and adjuvant died, compared with none in the other groups. There was more significant lung inflammation associated with eosinophilic infiltration, as well as increased levels of interleukin-4 (IL-4) and IL-5, in the bronchoalveolar lavages of mice immunized with hMPV alone or with the adjuvant. Mice from the last two groups had a 4-5 log10 decrease in their pulmonary viral titres compared with controls. Our data demonstrate the risks associated with immunization using inactivated hMPV in this animal model and that this aberrant response should be considered in the development of hMPV vaccines.

  4. DC-SIGN and L-SIGN Are Attachment Factors That Promote Infection of Target Cells by Human Metapneumovirus in the Presence or Absence of Cellular Glycosaminoglycans

    Science.gov (United States)

    Gillespie, Leah; Gerstenberg, Kathleen; Ana-Sosa-Batiz, Fernanda; Parsons, Matthew S.; Farrukee, Rubaiyea; Krabbe, Mark; Spann, Kirsten; Brooks, Andrew G.; Londrigan, Sarah L.

    2016-01-01

    ABSTRACT It is well established that glycosaminoglycans (GAGs) function as attachment factors for human metapneumovirus (HMPV), concentrating virions at the cell surface to promote interaction with other receptors for virus entry and infection. There is increasing evidence to suggest that multiple receptors may exhibit the capacity to promote infectious entry of HMPV into host cells; however, definitive identification of specific transmembrane receptors for HMPV attachment and entry is complicated by the widespread expression of cell surface GAGs. pgsA745 Chinese hamster ovary (CHO) cells are deficient in the expression of cell surface GAGs and resistant to HMPV infection. Here, we demonstrate that the expression of the Ca2+-dependent C-type lectin receptor (CLR) DC-SIGN (CD209L) or L-SIGN (CD209L) rendered pgsA745 cells permissive to HMPV infection. Unlike infection of parental CHO cells, HMPV infection of pgsA745 cells expressing DC-SIGN or L-SIGN was dynamin dependent and inhibited by mannan but not by pretreatment with bacterial heparinase. Parental CHO cells expressing DC-SIGN/L-SIGN also showed enhanced susceptibility to dynamin-dependent HMPV infection, confirming that CLRs can promote HMPV infection in the presence or absence of GAGs. Comparison of pgsA745 cells expressing wild-type and endocytosis-defective mutants of DC-SIGN/L-SIGN indicated that the endocytic function of CLRs was not essential but could contribute to HMPV infection of GAG-deficient cells. Together, these studies confirm a role for CLRs as attachment factors and entry receptors for HMPV infection. Moreover, they define an experimental system that can be exploited to identify transmembrane receptors and entry pathways where permissivity to HMPV infection can be rescued following the expression of a single cell surface receptor. IMPORTANCE On the surface of CHO cells, glycosaminoglycans (GAGs) function as the major attachment factor for human metapneumoviruses (HMPV), promoting dynamin

  5. Human metapneumovirus found in clinical materials of children with respiratory tract diseases

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    Katarina Logar

    2006-10-01

    Full Text Available Background: Human metapneumovirus (hMPV was first recognized in the Netherlands in 2001. Since then, it has been documented all over the world as a cause of human respiratory infections in all age groups. The objective of this study was to introduce and optimize an assay for detecting hMPV in clinical material of our patients. To date, there has not been a report that describes the detection of this virus in Slovenia.Methods: A total of 58 specimens, randomly collected during 2003/2004 from the patients ≤ 19 years old with respiratory disease and 20 specimens collected in 1997 were tested for hMPV. Extraction of RNA from frozen specimens and subsequent single-step reverse transcription-polymerase chain reaction (RT-PCR were performed. Human metapneumovirus amplicons were determined by electrophoresis in a 2 % (w/v agarose gel.Results: Human metapneumovirus was detected in 13/58 (22 % specimens; 10/40 (25 % specimens were from the upper and 3/18 (17 % from the lower respiratory tract. The mean age of infected patients was 3.2 ± 2.2 years. Out of 13 of the hMPV-positive specimens, 9 were positive also for another respiratory virus. Two of 20 (10 % archival specimens were hMPV-positive.Conclusions: This study is the first report about hMPV in Slovenia. Human metapneumovirus was detected as the second most frequent virus after RSV in children < 3 years of age. The virus was not found in the specimens from the children younger than 2 months. Based on the hMPV-positive results in archival clinical material, it is suggested that hMPV had circulated in Slovenia before the time it was discovered.

  6. Serologic evidence of avian metapneumovirus infection among adults occupationally exposed to Turkeys.

    Science.gov (United States)

    Kayali, Ghazi; Ortiz, Ernesto J; Chorazy, Margaret L; Nagaraja, Kakambi V; DeBeauchamp, Jennifer; Webby, Richard J; Gray, Gregory C

    2011-11-01

    Genetically similar, the avian metapneumovirus (aMPV) and the human MPV (hMPV) are the only viruses in the Metapneumovirus genus. Previous research demonstrated the ability of hMPV to cause clinical disease in turkeys. In this controlled, cross-sectional, seroepidemiological study, we examined the hypothesis that aMPV might infect humans. We enrolled 95 adults occupationally exposed to turkeys and 82 nonexposed controls. Sera from study participants were examined for antibodies against aMPV and hMPV. Both in bivariate (OR=3.2; 95% CI: 1.1-9.2) and in multivariate modelling adjusting for antibody to hMPV (OR=4.1; 95% CI: 1.3-13.1), meat-processing workers were found to have an increased odds of previous infection with aMPV compared to controls. While hMPV antibody cross-reactivity is evident, these data suggest that occupational exposure to turkeys is a risk factor for human infection with aMPV. More studies are needed to validate these findings, to identify modes of aMPV transmission, and to determine risk factors associated with infection.

  7. Development and optimization of a direct plaque assay for human and avian metapneumoviruses

    Science.gov (United States)

    Zhang, Yu; Wei, Yongwei; Li, Junan; Li, Jianrong

    2012-01-01

    The genus Metapneumovirus within the subfamily Pneumovirinae and family Paramyxoviridae includes only two viruses, human metapneumovirus (hMPV) and avian metapneumovirus (aMPV), which cause respiratory disease in humans and birds, respectively. These two viruses grow poorly in cell culture and other quantitation methods, such as indirect immuno-staining and immuno-fluorescent assays, are expensive, time consuming, and do not allow for plaque purification of the virus. In order to enhance research efforts for studying these two viruses, a direct plaque assay for both hMPV and aMPV has been developed. By optimizing the chemical components of the agarose overlay, it was found that both hMPV with a trypsin-independent F cleavage site and aMPV formed clear and countable plaques in a number of mammalian cell lines (such as Vero-E6 and LLC-MK2 cells) after 5 days of incubation. The plaque forming assay has similar sensitivity and reliability as the currently used immunological methods for viral quantitation. The plaque assay is also a more simple, rapid, and economical method compared to immunological assays, and in addition allows for plaque purification of the viruses. The direct plaque assay will be a valuable method for the quantitation and evaluation of the biological properties of some metapneumoviruses. PMID:22684013

  8. Incidence, Morbidity, and Costs of Human Metapneumovirus Infection in Hospitalized Children.

    Science.gov (United States)

    Davis, Carly R; Stockmann, Chris; Pavia, Andrew T; Byington, Carrie L; Blaschke, Anne J; Hersh, Adam L; Thorell, Emily A; Korgenski, Kent; Daly, Judy; Ampofo, Krow

    2016-09-01

    Human metapneumovirus (HMPV) causes acute respiratory tract infections in infants and children. We sought to measure the clinical and economic burden of HMPV infection in hospitalized children. We conducted a retrospective cohort study from 2007 to 2013 at Primary Children's Hospital in Salt Lake City, Utah. Children <18 years of age with laboratory-confirmed HMPV infection were included. Demographic, clinical, and financial data were abstracted from the electronic medical record. During the study period, 815 children were hospitalized with laboratory-confirmed HMPV infection: 16% <6 months, 50% 6-23 months, 23% 2-4 years, and 11% 5-17 years of age. A complex chronic condition was identified in 453 (56%) children hospitalized with HMPV infection; this proportion increased with increasing age (P < .001). There was marked variation in annual HMPV hospitalization rates, ranging from 9 of 100 000 person-years in 2012-2013 to 79 of 100 000 in 2009-2010. Hospitalization rates were highest among children <2 years (200 of 100 000 person-years) and lowest among children 5-17 years of age (5 of 100 000). Of hospitalized children, 18% were treated in the intensive care unit and 6% required mechanical ventilation. The median length of stay was 2.8 days (interquartile range [IQR], 1.8-4.6) and did not vary by age. The median total hospital cost per patient was $5513 (IQR, $3850-$9946) with significantly higher costs for patients with chronic medical conditions (P < .001). Human metapneumovirus infection results in a large number of hospitalizations with substantial morbidity, resource utilization, and costs. The development of a safe and effective vaccine could reduce the clinical and economic burden of HMPV. © The Author 2015. Published by Oxford University Press on behalf of the Pediatric Infectious Diseases Society. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  9. siRNA against the G gene of human metapneumovirus

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    Preston Faith

    2012-07-01

    Full Text Available Abstract Background Human metapneumovirus (hMPV is a significant viral respiratory pathogen of infants and children, the elderly and immunocompromised individuals. Disease associated with hMPV infection resembles that of human respiratory syncytial virus (RSV and includes bronchiolitis and pneumonia. The glycosylated G attachment protein of hMPV is required for viral entry in vivo and has also been identified as an inhibitor of innate immune responses. Findings We designed and validated two siRNA molecules against the G gene using A549 cells and demonstrated consistent 88-92% knock-down for one siRNA molecule, which was used in subsequent experiments. Significant reduction of G mRNA in A549 cells infected with hMPV did not result in a reduction in viral growth, nor did it significantly increase the production of type I interferon (α/β in response to infection. However, there was a moderate increase in IFN-β mRNA expression in response to infection in siG-transfected cells compared to untransfected and si-mismatch-transfected cells. Expression of G by recombinant adenovirus did not affect type I IFN expression. Conclusion G has been previously described as a type I interferon antagonist, although our findings suggest it may not be a significant antagonist.

  10. Human Metapneumovirus Induces Formation of Inclusion Bodies for Efficient Genome Replication and Transcription.

    Science.gov (United States)

    Cifuentes-Muñoz, Nicolás; Branttie, Jean; Slaughter, Kerri Beth; Dutch, Rebecca Ellis

    2017-12-15

    Human metapneumovirus (HMPV) causes significant upper and lower respiratory disease in all age groups worldwide. The virus possesses a negative-sense single-stranded RNA genome of approximately 13.3 kb encapsidated by multiple copies of the nucleoprotein (N), giving rise to helical nucleocapsids. In addition, copies of the phosphoprotein (P) and the large RNA polymerase (L) decorate the viral nucleocapsids. After viral attachment, endocytosis, and fusion mediated by the viral glycoproteins, HMPV nucleocapsids are released into the cell cytoplasm. To visualize the subsequent steps of genome transcription and replication, a fluorescence in situ hybridization (FISH) protocol was established to detect different viral RNA subpopulations in infected cells. The FISH probes were specific for detection of HMPV positive-sense RNA (+RNA) and viral genomic RNA (vRNA). Time course analysis of human bronchial epithelial BEAS-2B cells infected with HMPV revealed the formation of inclusion bodies (IBs) from early times postinfection. HMPV IBs were shown to be cytoplasmic sites of active transcription and replication, with the translation of viral proteins being closely associated. Inclusion body formation was consistent with an actin-dependent coalescence of multiple early replicative sites. Time course quantitative reverse transcription-PCR analysis suggested that the coalescence of inclusion bodies is a strategy to efficiently replicate and transcribe the viral genome. These results provide a better understanding of the steps following HMPV entry and have important clinical implications. IMPORTANCE Human metapneumovirus (HMPV) is a recently discovered pathogen that affects human populations of all ages worldwide. Reinfections are common throughout life, but no vaccines or antiviral treatments are currently available. In this work, a spatiotemporal analysis of HMPV replication and transcription in bronchial epithelial cell-derived immortal cells was performed. HMPV was shown to

  11. Analysis of antigenic cross-reactivity between subgroup C avian pneumovirus and human metapneumovirus by using recombinant fusion proteins.

    Science.gov (United States)

    Luo, L; Sabara, M I; Li, Y

    2009-10-01

    Avian pneumovirus subgroup C (APV/C) has recently been reported to be more closely related to human metapneumovirus (hMPV) as determined by sequence analysis. To examine the antigenic relationship between APV/C and hMPV, the APV/C fusion (F) gene was cloned and expressed as an uncleaved glycoprotein in a baculovirus system. The reactivity of the APV/C F protein with antibodies against APV subgroups A, B, C, and hMPV was examined by Western blot analysis. The results showed that the expressed APV/C F protein was not only recognized by APV/C-specific antibodies but also by antibodies raised against hMPV. Previously expressed recombinant hMPV F protein also reacted with APV/C-specific antibodies, suggesting that there was significant antigenic cross-reactivity and a potential evolutionary relationship between hMPV and APV/C. Interestingly, the recombinant F proteins from APV/C and hMPV were not recognized by polyclonal antibodies specific to APV subgroups A and B.

  12. Genetic variability of attachment (G and Fusion (F protein genes of human metapneumovirus strains circulating during 2006-2009 in Kolkata, Eastern India

    Directory of Open Access Journals (Sweden)

    Chawla-Sarkar Mamta

    2011-02-01

    Full Text Available Abstract Background Human metapneumovirus (hMPV is associated with the acute respiratory tract infection (ARTI in all the age groups. However, there is limited information on prevalence and genetic diversity of human metapneumovirus (hMPV strains circulating in India. Objective To study prevalence and genomic diversity of hMPV strains among ARTI patients reporting in outpatient departments of hospitals in Kolkata, Eastern India. Methods Nasal and/or throat swabs from 2309 patients during January 2006 to December 2009, were screened for the presence of hMPV by RT-PCR of nucleocapsid (N gene. The G and F genes of representative hMPV positive samples were sequenced. Results 118 of 2309 (5.11% clinical samples were positive for hMPV. The majority (≈80% of the positive cases were detected during July−November all through the study period. Genetic analysis revealed that 77% strains belong to A2 subgroup whereas rest clustered in B1 subgroup. G sequences showed higher diversity at the nucleotide and amino acid level. In contrast, less than 10% variation was observed in F gene of representative strains of all four years. Sequence analysis also revealed changes in the position of stop codon in G protein, which resulted in variable length (217-231 aa polypeptides. Conclusion The study suggests that approximately 5% of ARTI in the region were caused by hMPV. This is the first report on the genetic variability of G and F gene of hMPV strains from India which clearly shows that the G protein of hMPV is continuously evolving. Though the study partially fulfills lacunae of information, further studies from other regions are necessary for better understanding of prevalence, epidemiology and virus evolution in Indian subcontinent.

  13. The cellular endosomal sorting complex required for transport pathway is not involved in avian metapneumovirus budding in a virus-like-particle expression system.

    Science.gov (United States)

    Weng, Yuejin; Lu, Wuxun; Harmon, Aaron; Xiang, Xiaoxiao; Deng, Qiji; Song, Minxun; Wang, Dan; Yu, Qingzhong; Li, Feng

    2011-05-01

    Avian metapneumovirus (AMPV) is a paramyxovirus that principally causes respiratory disease and egg production drops in turkeys and chickens. Together with its closely related human metapneumovirus (HMPV), they comprise the genus Metapneumovirus in the family Paramyxoviridae. Little is currently known about the mechanisms involved in the budding of metapneumovirus. By using AMPV as a model system, we showed that the matrix (M) protein by itself was insufficient to form virus-like-particles (VLPs). The incorporation of M into VLPs was shown to occur only when both the viral nucleoprotein (N) and the fusion (F) proteins were co-expressed. Furthermore, we provided evidence indicating that two YSKL and YAGL segments encoded within the M protein were not a functional late domain, and the endosomal sorting complex required for transport (ESCRT) machinery was not involved in metapneumovirus budding, consistent with a recent observation that human respiratory syncytial virus, closely related to HMPV, uses an ESCRT-independent budding mechanism. Taken together, these results suggest that metapneumovirus budding is independent of the ESCRT pathway and the minimal budding machinery described here will aid our future understanding of metapneumovirus assembly and egress.

  14. Human Metapneumovirus Infection is Associated with Severe Respiratory Disease in Preschool Children with History of Prematurity.

    Science.gov (United States)

    Pancham, Krishna; Sami, Iman; Perez, Geovanny F; Huseni, Shehlanoor; Kurdi, Bassem; Rose, Mary C; Rodriguez-Martinez, Carlos E; Nino, Gustavo

    2016-02-01

    Human metapneumovirus (HMPV) is a recently discovered respiratory pathogen of the family Paramyxoviridae, the same family as that of respiratory syncytial virus (RSV). Premature children are at high risk of severe RSV infections, however, it is unclear whether HMPV infection is more severe in hospitalized children with a history of severe prematurity. We conducted a retrospective analysis of the clinical respiratory presentation of all polymerase chain reaction-confirmed HMPV infections in preschool-age children (≤5 years) with and without history of severe prematurity (prematurity. Preschool children with a history of prematurity had more severe HMPV disease as illustrated by longer hospitalizations, new or increased need for supplemental O2, and higher severity scores independently of age, ethnicity, and history of asthma. Our study suggests that HMPV infection causes significant disease burden among preschool children with a history of prematurity leading to severe respiratory infections and increasing health care resource utilization due to prolonged hospitalizations. Copyright © 2016. Published by Elsevier B.V.

  15. Identification of human metapneumovirus-induced gene networks in airway epithelial cells by microarray analysis

    International Nuclear Information System (INIS)

    Bao, X.; Sinha, M.; Liu, T.; Hong, C.; Luxon, B.A.; Garofalo, R.P.; Casola, A.

    2008-01-01

    Human metapneumovirus (hMPV) is a major cause of lower respiratory tract infections in infants, elderly and immunocompromised patients. Little is known about the response to hMPV infection of airway epithelial cells, which play a pivotal role in initiating and shaping innate and adaptive immune responses. In this study, we analyzed the transcriptional profiles of airway epithelial cells infected with hMPV using high-density oligonucleotide microarrays. Of the 47,400 transcripts and variants represented on the Affimetrix GeneChip Human Genome HG-U133 plus 2 array, 1601 genes were significantly altered following hMPV infection. Altered genes were then assigned to functional categories and mapped to signaling pathways. Many up-regulated genes are involved in the initiation of pro-inflammatory and antiviral immune responses, including chemokines, cytokines, type I interferon and interferon-inducible proteins. Other important functional classes up-regulated by hMPV infection include cellular signaling, gene transcription and apoptosis. Notably, genes associated with antioxidant and membrane transport activity, several metabolic pathways and cell proliferation were down-regulated in response to hMPV infection. Real-time PCR and Western blot assays were used to confirm the expression of genes related to several of these functional groups. The overall result of this study provides novel information on host gene expression upon infection with hMPV and also serves as a foundation for future investigations of genes and pathways involved in the pathogenesis of this important viral infection. Furthermore, it can facilitate a comparative analysis of other paramyxoviral infections to determine the transcriptional changes that are conserved versus the one that are specific to individual pathogens

  16. Effects of human metapneumovirus and respiratory syncytial virus antigen insertion in two 3' proximal genome positions of bovine/human parainfluenza virus type 3 on virus replication and immunogenicity

    NARCIS (Netherlands)

    R.S. Tang (Roderick); J.H. Schickli (Jeanne); M. MacPhail (Mia); F. Fernandes (Fiona); L. Bicha (Leenas); J. Spaete (Joshua); R.A.M. Fouchier (Ron); A.D.M.E. Osterhaus (Albert); R. Spaete (Richard); A.A. Haller (Aurelia)

    2003-01-01

    textabstractA live attenuated bovine parainfluenza virus type 3 (PIV3), harboring the fusion (F) and hemagglutinin-neuraminidase (HN) genes of human PIV3, was used as a virus vector to express surface glycoproteins derived from two human pathogens, human metapneumovirus (hMPV) and respiratory

  17. Localization of a region in the fusion protein of avian metapneumovirus that modulates cell-cell fusion.

    Science.gov (United States)

    Wei, Yongwei; Feng, Kurtis; Yao, Xiangjie; Cai, Hui; Li, Junan; Mirza, Anne M; Iorio, Ronald M; Li, Jianrong

    2012-11-01

    The genus Metapneumovirus within the subfamily Pneumovirinae of the family Paramyxoviridae includes two members, human metapneumovirus (hMPV) and avian metapneumovirus (aMPV), causing respiratory tract infections in humans and birds, respectively. Paramyxoviruses enter host cells by fusing the viral envelope with a host cell membrane. Membrane fusion of hMPV appears to be unique, in that fusion of some hMPV strains requires low pH. Here, we show that the fusion (F) proteins of aMPV promote fusion in the absence of the attachment protein and low pH is not required. Furthermore, there are notable differences in cell-cell fusion among aMPV subtypes. Trypsin was required for cell-cell fusion induced by subtype B but not subtypes A and C. The F protein of aMPV subtype A was highly fusogenic, whereas those from subtypes B and C were not. By construction and evaluation of chimeric F proteins composed of domains from the F proteins of subtypes A and B, we localized a region composed of amino acid residues 170 to 338 in the F protein that is responsible for the hyperfusogenic phenotype of the F from subtype A. Further mutagenesis analysis revealed that residues R295, G297, and K323 in this region collectively contributed to the hyperfusogenicity. Taken together, we have identified a region in the aMPV F protein that modulates the extent of membrane fusion. A model for fusion consistent with these data is presented.

  18. Recovery of avian metapneumovirus subgroup C from cDNA: cross-recognition of avian and human metapneumovirus support proteins.

    Science.gov (United States)

    Govindarajan, Dhanasekaran; Buchholz, Ursula J; Samal, Siba K

    2006-06-01

    Avian metapneumovirus (AMPV) causes an acute respiratory disease in turkeys and is associated with "swollen head syndrome" in chickens, contributing to significant economic losses for the U.S. poultry industry. With a long-term goal of developing a better vaccine for controlling AMPV in the United States, we established a reverse genetics system to produce infectious AMPV of subgroup C entirely from cDNA. A cDNA clone encoding the entire 14,150-nucleotide genome of AMPV subgroup C strain Colorado (AMPV/CO) was generated by assembling five cDNA fragments between the T7 RNA polymerase promoter and the autocatalytic hepatitis delta virus ribozyme of a transcription plasmid, pBR 322. Transfection of this plasmid, along with the expression plasmids encoding the N, P, M2-1, and L proteins of AMPV/CO, into cells stably expressing T7 RNA polymerase resulted in the recovery of infectious AMPV/CO. Characterization of the recombinant AMPV/CO showed that its growth properties in tissue culture were similar to those of the parental virus. The potential of AMPV/CO to serve as a viral vector was also assessed by generating another recombinant virus, rAMPV/CO-GFP, that expressed the enhanced green fluorescent protein (GFP) as a foreign protein. Interestingly, GFP-expressing AMPV and GFP-expressing human metapneumovirus (HMPV) could be recovered using the support plasmids of either virus, denoting that the genome promoters are conserved between the two metapneumoviruses and can be cross-recognized by the polymerase complex proteins of either virus. These results indicate a close functional relationship between AMPV/CO and HMPV.

  19. Evidence for the interaction of the human metapneumovirus G and F proteins during virus-like particle formation.

    Science.gov (United States)

    Loo, Liat Hui; Jumat, Muhammad Raihan; Fu, Yi; Ayi, Teck Choon; Wong, Pui San; Tee, Nancy W S; Tan, Boon Huan; Sugrue, Richard J

    2013-09-25

    Human metapneumovirus (HMPV) is now a major cause of lower respiratory infection in children. Although primary isolation of HMPV has been achieved in several different cell lines, the low level of virus replication and the subsequent recovery of low levels of infectious HMPV have hampered biochemical studies on the virus. These experimental methodologies usually require higher levels of biological material that can be achieved following HMPV infection. In this study we demonstrate that expression of the HMPV F, G and M proteins in mammalian cells leads to HMPV virus-like particles (VLP) formation. This experimental strategy will serve as a model system to allow the process of HMPV virus assembly to be examined. The HMPV F, G and M proteins were expressed in mammalian cell lines. Protein cross-linking studies, sucrose gradient centrifugation and in situ imaging was used to examine interactions between the virus proteins. VLP formation was examined using sucrose density gradient centrifugation and electron microscopy analysis. Analysis of cells co-expressing the F, G and M proteins demonstrated that these proteins interacted. Furthermore, in cells co-expression the three HMPV proteins the formation VLPs was observed. Image analysis revealed the VLPs had a similar morphology to the filamentous virus morphology that we observed on HMPV-infected cells. The capacity of each protein to initiate VLP formation was examined using a VLP formation assay. Individual expression of each virus protein showed that the G protein was able to form VLPs in the absence of the other virus proteins. Furthermore, co-expression of the G protein with either the M or F proteins facilitated their incorporation into the VLP fraction. Co-expression of the F, G and M proteins leads to the formation of VLPs, and that incorporation of the F and M proteins into VLPs is facilitated by their interaction with the G protein. Our data suggests that the G protein plays a central role in VLP formation, and

  20. Phosphorylation of Human Metapneumovirus M2-1 Protein Upregulates Viral Replication and Pathogenesis.

    Science.gov (United States)

    Cai, Hui; Zhang, Yu; Lu, Mijia; Liang, Xueya; Jennings, Ryan; Niewiesk, Stefan; Li, Jianrong

    2016-08-15

    Human metapneumovirus (hMPV) is a major causative agent of upper- and lower-respiratory-tract infections in infants, the elderly, and immunocompromised individuals worldwide. Like all pneumoviruses, hMPV encodes the zinc binding protein M2-1, which plays important regulatory roles in RNA synthesis. The M2-1 protein is phosphorylated, but the specific role(s) of the phosphorylation in viral replication and pathogenesis remains unknown. In this study, we found that hMPV M2-1 is phosphorylated at amino acid residues S57 and S60. Subsequent mutagenesis found that phosphorylation is not essential for zinc binding activity and oligomerization, whereas inhibition of zinc binding activity abolished the phosphorylation and oligomerization of the M2-1 protein. Using a reverse genetics system, recombinant hMPVs (rhMPVs) lacking either one or both phosphorylation sites in the M2-1 protein were recovered. These recombinant viruses had a significant decrease in both genomic RNA replication and mRNA transcription. In addition, these recombinant viruses were highly attenuated in cell culture and cotton rats. Importantly, rhMPVs lacking phosphorylation in the M2-1 protein triggered high levels of neutralizing antibody and provided complete protection against challenge with wild-type hMPV. Collectively, these data demonstrated that phosphorylation of the M2-1 protein upregulates hMPV RNA synthesis, replication, and pathogenesis in vivo The pneumoviruses include many important human and animal pathogens, such as human respiratory syncytial virus (hRSV), hMPV, bovine RSV, and avian metapneumovirus (aMPV). Among these viruses, hRSV and hMPV are the leading causes of acute respiratory tract infection in infants and children. Currently, there is no antiviral or vaccine to combat these diseases. All known pneumoviruses encode a zinc binding protein, M2-1, which is a transcriptional antitermination factor. In this work, we found that phosphorylation of M2-1 is essential for virus

  1. Functional motifs responsible for human metapneumovirus M2-2-mediated innate immune evasion.

    Science.gov (United States)

    Chen, Yu; Deng, Xiaoling; Deng, Junfang; Zhou, Jiehua; Ren, Yuping; Liu, Shengxuan; Prusak, Deborah J; Wood, Thomas G; Bao, Xiaoyong

    2016-12-01

    Human metapneumovirus (hMPV) is a major cause of lower respiratory infection in young children. Repeated infections occur throughout life, but its immune evasion mechanisms are largely unknown. We recently found that hMPV M2-2 protein elicits immune evasion by targeting mitochondrial antiviral-signaling protein (MAVS), an antiviral signaling molecule. However, the molecular mechanisms underlying such inhibition are not known. Our mutagenesis studies revealed that PDZ-binding motifs, 29-DEMI-32 and 39-KEALSDGI-46, located in an immune inhibitory region of M2-2, are responsible for M2-2-mediated immune evasion. We also found both motifs prevent TRAF5 and TRAF6, the MAVS downstream adaptors, to be recruited to MAVS, while the motif 39-KEALSDGI-46 also blocks TRAF3 migrating to MAVS. In parallel, these TRAFs are important in activating transcription factors NF-kB and/or IRF-3 by hMPV. Our findings collectively demonstrate that M2-2 uses its PDZ motifs to launch the hMPV immune evasion through blocking the interaction of MAVS and its downstream TRAFs. Copyright © 2016 Elsevier Inc. All rights reserved.

  2. Genetic diversity, seasonality and transmission network of human metapneumovirus: identification of a unique sub-lineage of the fusion and attachment genes.

    Science.gov (United States)

    Chow, Wei Zhen; Chan, Yoke Fun; Oong, Xiang Yong; Ng, Liang Jie; Nor'E, Siti Sarah; Ng, Kim Tien; Chan, Kok Gan; Hanafi, Nik Sherina; Pang, Yong Kek; Kamarulzaman, Adeeba; Tee, Kok Keng

    2016-06-09

    Human metapneumovirus (HMPV) is an important viral respiratory pathogen worldwide. Current knowledge regarding the genetic diversity, seasonality and transmission dynamics of HMPV among adults and children living in tropical climate remains limited. HMPV prevailed at 2.2% (n = 86/3,935) among individuals presented with acute respiratory tract infections in Kuala Lumpur, Malaysia between 2012 and 2014. Seasonal peaks were observed during the northeast monsoon season (November-April) and correlated with higher relative humidity and number of rainy days (P A2 was identified in 18.6% (16/86) of the population. Using a custom script for network construction based on the TN93 pairwise genetic distance, we identified up to nine HMPV transmission clusters circulating as multiple sub-epidemics. Although no apparent major outbreak was observed, the increased frequency of transmission clusters (dyads) during seasonal peaks suggests the potential roles of transmission clusters in driving the spread of HMPV. Our findings provide essential information for therapeutic research, prevention strategies, and disease outbreak monitoring of HMPV.

  3. Genetic diversity, seasonality and transmission network of human metapneumovirus: identification of a unique sub-lineage of the fusion and attachment genes

    Science.gov (United States)

    Chow, Wei Zhen; Chan, Yoke Fun; Oong, Xiang Yong; Ng, Liang Jie; Nor’E, Siti Sarah; Ng, Kim Tien; Chan, Kok Gan; Hanafi, Nik Sherina; Pang, Yong Kek; Kamarulzaman, Adeeba; Tee, Kok Keng

    2016-01-01

    Human metapneumovirus (HMPV) is an important viral respiratory pathogen worldwide. Current knowledge regarding the genetic diversity, seasonality and transmission dynamics of HMPV among adults and children living in tropical climate remains limited. HMPV prevailed at 2.2% (n = 86/3,935) among individuals presented with acute respiratory tract infections in Kuala Lumpur, Malaysia between 2012 and 2014. Seasonal peaks were observed during the northeast monsoon season (November–April) and correlated with higher relative humidity and number of rainy days (P population. Using a custom script for network construction based on the TN93 pairwise genetic distance, we identified up to nine HMPV transmission clusters circulating as multiple sub-epidemics. Although no apparent major outbreak was observed, the increased frequency of transmission clusters (dyads) during seasonal peaks suggests the potential roles of transmission clusters in driving the spread of HMPV. Our findings provide essential information for therapeutic research, prevention strategies, and disease outbreak monitoring of HMPV. PMID:27279080

  4. Genome-Wide Analysis of Human Metapneumovirus Evolution.

    Directory of Open Access Journals (Sweden)

    Jin Il Kim

    Full Text Available Human metapneumovirus (HMPV has been described as an important etiologic agent of upper and lower respiratory tract infections, especially in young children and the elderly. Most of school-aged children might be introduced to HMPVs, and exacerbation with other viral or bacterial super-infection is common. However, our understanding of the molecular evolution of HMPVs remains limited. To address the comprehensive evolutionary dynamics of HMPVs, we report a genome-wide analysis of the eight genes (N, P, M, F, M2, SH, G, and L using 103 complete genome sequences. Phylogenetic reconstruction revealed that the eight genes from one HMPV strain grouped into the same genetic group among the five distinct lineages (A1, A2a, A2b, B1, and B2. A few exceptions of phylogenetic incongruence might suggest past recombination events, and we detected possible recombination breakpoints in the F, SH, and G coding regions. The five genetic lineages of HMPVs shared quite remote common ancestors ranging more than 220 to 470 years of age with the most recent origins for the A2b sublineage. Purifying selection was common, but most protein genes except the F and M2-2 coding regions also appeared to experience episodic diversifying selection. Taken together, these suggest that the five lineages of HMPVs maintain their individual evolutionary dynamics and that recombination and selection forces might work on shaping the genetic diversity of HMPVs.

  5. The human metapneumovirus matrix protein stimulates the inflammatory immune response in vitro.

    Directory of Open Access Journals (Sweden)

    Audrey Bagnaud-Baule

    Full Text Available Each year, during winter months, human Metapneumovirus (hMPV is associated with epidemics of bronchiolitis resulting in the hospitalization of many infants. Bronchiolitis is an acute illness of the lower respiratory tract with a consequent inflammation of the bronchioles. The rapid onset of inflammation suggests the innate immune response may have a role to play in the pathogenesis of this hMPV infection. Since, the matrix protein is one of the most abundant proteins in the Paramyxoviridae family virion, we hypothesized that the inflammatory modulation observed in hMPV infected patients may be partly associated with the matrix protein (M-hMPV response. By western blot analysis, we detected a soluble form of M-hMPV released from hMPV infected cell as well as from M-hMPV transfected HEK 293T cells suggesting that M-hMPV may be directly in contact with antigen presenting cells (APCs during the course of infection. Moreover, flow cytometry and confocal microscopy allowed determining that M-hMPV was taken up by dendritic cells (moDCs and macrophages inducing their activation. Furthermore, these moDCs enter into a maturation process inducing the secretion of a broad range of inflammatory cytokines when exposed to M-hMPV. Additionally, M-hMPV activated DCs were shown to stimulate IL-2 and IFN-γ production by allogeneic T lymphocytes. This M-hMPV-mediated activation and antigen presentation of APCs may in part explain the marked inflammatory immune response observed in pathology induced by hMPV in patients.

  6. Prospective Evaluation of Rapid Antigen Tests for Diagnosis of Respiratory Syncytial Virus and Human Metapneumovirus Infections▿

    Science.gov (United States)

    Aslanzadeh, Jaber; Zheng, Xiaotian; Li, Haijing; Tetreault, Janice; Ratkiewicz, Irene; Meng, Shufang; Hamilton, Pamela; Tang, Yi-Wei

    2008-01-01

    Respiratory syncytial virus (RSV) and human metapneumovirus (hMPV) are two important viral pathogens that cause respiratory tract infections in the pediatric population. The rapid detection of these agents allows the prompt isolation and treatment of infected patients. In the present prospective study, we evaluated the performances of four rapid antigen detection assays, including a rapid chromatographic immunoassay (CIA) for RSV (Directigen EZ RSV; Becton Dickinson, Sparks, MD), a direct fluorescent-antibody assay (DFA) for RSV (Bartels; Trinity Biotech, Carlsbad, CA), and two DFAs for hMPV manufactured by Diagnostic Hybrids Inc. (DHI; Athens, OH) and Imagen (Oxoid Ltd., Basingstoke, Hampshire, United Kingdom). The clinical specimens tested comprised 515 nasopharyngeal aspirates submitted to the Clinical Microbiology Laboratory at Hartford Hospital from 1 November 2006 to 21 April 2007. Compared to the results of real-time reverse transcription-PCR (RT-PCR), the CIA had a sensitivity of 79.8% and a specificity of 89.5%. The RSV DFA with Bartels reagents showed a sensitivity of 94.1% and a specificity of 96.8%. For hMPV, the sensitivity and specificity were 62.5% and 99.8%, respectively, for the DHI DFA and 63.2% and 100%, respectively, for the Imagen DFA. The hands-on and test turnaround times for CIA were 10 and 30 to 60 min, respectively, and the hands-on and test turnaround times for the RSV and hMPV DFAs were 30 and 105 min, respectively. We conclude that while the RSV CIA is user-friendly, it lacks sensitivity and specificity, especially during off-peak months. In contrast, the RSV DFA is more sensitive and specific, but interpretation of its results is subjective and it demands technical time and expertise. Similarly, both hMPV DFAs are highly specific in comparison to the results of RT-PCR, but their sensitivities await further improvements. PMID:18337386

  7. Prospective evaluation of rapid antigen tests for diagnosis of respiratory syncytial virus and human metapneumovirus infections.

    Science.gov (United States)

    Aslanzadeh, Jaber; Zheng, Xiaotian; Li, Haijing; Tetreault, Janice; Ratkiewicz, Irene; Meng, Shufang; Hamilton, Pamela; Tang, Yi-Wei

    2008-05-01

    Respiratory syncytial virus (RSV) and human metapneumovirus (hMPV) are two important viral pathogens that cause respiratory tract infections in the pediatric population. The rapid detection of these agents allows the prompt isolation and treatment of infected patients. In the present prospective study, we evaluated the performances of four rapid antigen detection assays, including a rapid chromatographic immunoassay (CIA) for RSV (Directigen EZ RSV; Becton Dickinson, Sparks, MD), a direct fluorescent-antibody assay (DFA) for RSV (Bartels; Trinity Biotech, Carlsbad, CA), and two DFAs for hMPV manufactured by Diagnostic Hybrids Inc. (DHI; Athens, OH) and Imagen (Oxoid Ltd., Basingstoke, Hampshire, United Kingdom). The clinical specimens tested comprised 515 nasopharyngeal aspirates submitted to the Clinical Microbiology Laboratory at Hartford Hospital from 1 November 2006 to 21 April 2007. Compared to the results of real-time reverse transcription-PCR (RT-PCR), the CIA had a sensitivity of 79.8% and a specificity of 89.5%. The RSV DFA with Bartels reagents showed a sensitivity of 94.1% and a specificity of 96.8%. For hMPV, the sensitivity and specificity were 62.5% and 99.8%, respectively, for the DHI DFA and 63.2% and 100%, respectively, for the Imagen DFA. The hands-on and test turnaround times for CIA were 10 and 30 to 60 min, respectively, and the hands-on and test turnaround times for the RSV and hMPV DFAs were 30 and 105 min, respectively. We conclude that while the RSV CIA is user-friendly, it lacks sensitivity and specificity, especially during off-peak months. In contrast, the RSV DFA is more sensitive and specific, but interpretation of its results is subjective and it demands technical time and expertise. Similarly, both hMPV DFAs are highly specific in comparison to the results of RT-PCR, but their sensitivities await further improvements.

  8. Human metapneumovirus M2-2 protein inhibits innate immune response in monocyte-derived dendritic cells.

    Directory of Open Access Journals (Sweden)

    Junping Ren

    Full Text Available Human metapneumovirus (hMPV is a leading cause of lower respiratory infection in young children, the elderly and immunocompromised patients. Repeated hMPV infections occur throughout life. However, immune evasion mechanisms of hMPV infection are largely unknown. Recently, our group has demonstrated that hMPV M2-2 protein, an important virulence factor, contributes to immune evasion in airway epithelial cells by targeting the mitochondrial antiviral-signaling protein (MAVS. Whether M2-2 regulates the innate immunity in human dendritic cells (DC, an important family of immune cells controlling antigen presenting, is currently unknown. We found that human DC infected with a virus lacking M2-2 protein expression (rhMPV-ΔM2-2 produced higher levels of cytokines, chemokines and IFNs, compared to cells infected with wild-type virus (rhMPV-WT, suggesting that M2-2 protein inhibits innate immunity in human DC. In parallel, we found that myeloid differentiation primary response gene 88 (MyD88, an essential adaptor for Toll-like receptors (TLRs, plays a critical role in inducing immune response of human DC, as downregulation of MyD88 by siRNA blocked the induction of immune regulatory molecules by hMPV. Since M2-2 is a cytoplasmic protein, we investigated whether M2-2 interferes with MyD88-mediated antiviral signaling. We found that indeed M2-2 protein associated with MyD88 and inhibited MyD88-dependent gene transcription. In this study, we also identified the domains of M2-2 responsible for its immune inhibitory function in human DC. In summary, our results demonstrate that M2-2 contributes to hMPV immune evasion by inhibiting MyD88-dependent cellular responses in human DC.

  9. Antiviral Activity of Favipiravir (T-705) against a Broad Range of Paramyxoviruses In Vitro and against Human Metapneumovirus in Hamsters.

    Science.gov (United States)

    Jochmans, D; van Nieuwkoop, S; Smits, S L; Neyts, J; Fouchier, R A M; van den Hoogen, B G

    2016-08-01

    The clinical impact of infections with respiratory viruses belonging to the family Paramyxoviridae argues for the development of antiviral therapies with broad-spectrum activity. Favipiravir (T-705) has demonstrated potent antiviral activity against multiple RNA virus families and is presently in clinical evaluation for the treatment of influenza. Here we demonstrate in vitro activity of T-705 against the paramyxoviruses human metapneumovirus (HMPV), respiratory syncytial virus, human parainfluenza virus, measles virus, Newcastle disease virus, and avian metapneumovirus. In addition, we demonstrate activity against HMPV in hamsters. T-705 treatment inhibited replication of all paramyxoviruses tested in vitro, with 90% effective concentration (EC90) values of 8 to 40 μM. Treatment of HMPV-challenged hamsters with T-705 at 200 mg/kg of body weight/day resulted in 100% protection from infection of the lungs. In all treated and challenged animals, viral RNA remained detectable in the respiratory tract. The observation that T-705 treatment had a significant effect on infectious viral titers, with a limited effect on viral genome titers, is in agreement with its proposed mode of action of viral mutagenesis. However, next-generation sequencing of viral genomes isolated from treated and challenged hamsters did not reveal (hyper)mutation. Polymerase activity assays revealed a specific effect of T-705 on the activity of the HMPV polymerase. With the reported antiviral activity of T-705 against a broad range of RNA virus families, this small molecule is a promising broad-range antiviral drug candidate for limiting the viral burden of paramyxoviruses and for evaluation for treatment of infections with (re)emerging viruses, such as the henipaviruses. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

  10. Localization of a Region in the Fusion Protein of Avian Metapneumovirus That Modulates Cell-Cell Fusion

    Science.gov (United States)

    Wei, Yongwei; Feng, Kurtis; Yao, Xiangjie; Cai, Hui; Li, Junan; Mirza, Anne M.; Iorio, Ronald M.

    2012-01-01

    The genus Metapneumovirus within the subfamily Pneumovirinae of the family Paramyxoviridae includes two members, human metapneumovirus (hMPV) and avian metapneumovirus (aMPV), causing respiratory tract infections in humans and birds, respectively. Paramyxoviruses enter host cells by fusing the viral envelope with a host cell membrane. Membrane fusion of hMPV appears to be unique, in that fusion of some hMPV strains requires low pH. Here, we show that the fusion (F) proteins of aMPV promote fusion in the absence of the attachment protein and low pH is not required. Furthermore, there are notable differences in cell-cell fusion among aMPV subtypes. Trypsin was required for cell-cell fusion induced by subtype B but not subtypes A and C. The F protein of aMPV subtype A was highly fusogenic, whereas those from subtypes B and C were not. By construction and evaluation of chimeric F proteins composed of domains from the F proteins of subtypes A and B, we localized a region composed of amino acid residues 170 to 338 in the F protein that is responsible for the hyperfusogenic phenotype of the F from subtype A. Further mutagenesis analysis revealed that residues R295, G297, and K323 in this region collectively contributed to the hyperfusogenicity. Taken together, we have identified a region in the aMPV F protein that modulates the extent of membrane fusion. A model for fusion consistent with these data is presented. PMID:22915815

  11. Deduced amino acid sequence of the small hydrophobic protein of US avian pneumovirus has greater identity with that of human metapneumovirus than those of non-US avian pneumoviruses.

    Science.gov (United States)

    Yunus, Abdul S; Govindarajan, Dhanasekaran; Huang, Zhuhui; Samal, Siba K

    2003-05-01

    We report here the nucleotide and deduced amino acid (aa) sequences of the small hydrophobic (SH) gene of the avian pneumovirus strain Colorado (APV/CO). The SH gene of APV/CO is 628 nucleotides in length from gene-start to gene-end. The longest ORF of the SH gene encoded a protein of 177 aas in length. Comparison of the deduced aa sequence of the SH protein of APV/CO with the corresponding published sequences of other members of genera metapneumovirus showed 28% identity with the newly discovered human metapneumovirus (hMPV), but no discernable identity with the APV subgroup A or B. Collectively, this data supports the hypothesis that: (i) APV/CO is distinct from European APV subgroups and belongs to the novel subgroup APV/C (APV/US); (ii) APV/CO is more closely related to hMPV, a mammalian metapneumovirus, than to either APV subgroup A or B. The SH gene of APV/CO was cloned using a genomic walk strategy which initiated cDNA synthesis from genomic RNA that traversed the genes in the order 3'-M-F-M2-SH-G-5', thus confirming that gene-order of APV/CO conforms in the genus Metapneumovirus. We also provide the sequences of transcription-signals and the M-F, F-M2, M2-SH and SH-G intergenic regions of APV/CO.

  12. Detection of Nuclear Protein Profile Changes by Human Metapneumovirus M2-2 Protein Using Quantitative Differential Proteomics

    Directory of Open Access Journals (Sweden)

    Yuping Ren

    2017-12-01

    Full Text Available Human metapneumovirus (hMPV is a leading cause of lower respiratory infection in pediatric populations globally. This study examined proteomic profile changes in A549 cells infected with hMPV and two attenuated mutants with deleted PDZ domain-binding motif(s in the M2-2 protein. These motifs are involved in the interruption of antiviral signaling, namely the interaction between the TNF receptor associated factor (TRAF and mitochondrial antiviral-signaling (MAVS proteins. The aim of this study was to provide insight into the overall and novel impact of M2-2 motifs on cellular responses via an unbiased comparison. Tandem mass tagging, stable isotope labeling, and high-resolution mass spectrometry were used for quantitative proteomic analysis. Using quantitative proteomics and Venn analysis, 1248 common proteins were detected in all infected samples of both technical sets. Hierarchical clustering of the differentiated proteome displayed distinct proteomic signatures that were controlled by the motif(s. Bioinformatics and experimental analysis confirmed the differentiated proteomes, revealed novel cellular biological events, and implicated key pathways controlled by hMPV M2-2 PDZ domain-binding motif(s. This provides further insight for evaluating M2-2 mutants as potent vaccine candidates.

  13. Single reaction, real time RT-PCR detection of all known avian and human metapneumoviruses.

    Science.gov (United States)

    Lemaitre, E; Allée, C; Vabret, A; Eterradossi, N; Brown, P A

    2018-01-01

    Current molecular methods for the detection of avian and human metapneumovirus (AMPV, HMPV) are specifically targeted towards each virus species or individual subgroups of these. Here a broad range SYBR Green I real time RT-PCR was developed which amplified a highly conserved fragment of sequence in the N open reading frame. This method was sufficiently efficient and specific in detecting all MPVs. Its validation according to the NF U47-600 norm for the four AMPV subgroups estimated low limits of detection between 1000 and 10copies/μL, similar with detection levels described previously for real time RT-PCRs targeting specific subgroups. RNA viruses present a challenge for the design of durable molecular diagnostic test due to the rate of change in their genome sequences which can vary substantially in different areas and over time. The fact that the regions of sequence for primer hybridization in the described method have remained sufficiently conserved since the AMPV and HMPV diverged, should give the best chance of continued detection of current subgroups and of potential unknown or future emerging MPV strains. Copyright © 2017 Elsevier B.V. All rights reserved.

  14. Solution and crystallographic structures of the central region of the phosphoprotein from human metapneumovirus.

    Directory of Open Access Journals (Sweden)

    Cedric Leyrat

    Full Text Available Human metapneumovirus (HMPV of the family Paramyxoviridae is a major cause of respiratory illness worldwide. Phosphoproteins (P from Paramyxoviridae are essential co-factors of the viral RNA polymerase that form tetramers and possess long intrinsically disordered regions (IDRs. We located the central region of HMPV P (P(ced which is involved in tetramerization using disorder analysis and modeled its 3D structure ab initio using Rosetta fold-and-dock. We characterized the solution-structure of P(ced using small angle X-ray scattering (SAXS and carried out direct fitting to the scattering data to filter out incorrect models. Molecular dynamics simulations (MDS and ensemble optimization were employed to select correct models and capture the dynamic character of P(ced. Our analysis revealed that oligomerization involves a compact central core located between residues 169-194 (P(core, that is surrounded by flexible regions with α-helical propensity. We crystallized this fragment and solved its structure at 3.1 Å resolution by molecular replacement, using the folded core from our SAXS-validated ab initio model. The RMSD between modeled and experimental tetramers is as low as 0.9 Å, demonstrating the accuracy of the approach. A comparison of the structure of HMPV P to existing mononegavirales P(ced structures suggests that P(ced evolved under weak selective pressure. Finally, we discuss the advantages of using SAXS in combination with ab initio modeling and MDS to solve the structure of small, homo-oligomeric protein complexes.

  15. Molecular epidemiology and evolution of human respiratory syncytial virus and human metapneumovirus.

    Directory of Open Access Journals (Sweden)

    Eleanor R Gaunt

    2011-03-01

    Full Text Available Human respiratory syncytial virus (HRSV and human metapneumovirus (HMPV are ubiquitous respiratory pathogens of the Pneumovirinae subfamily of the Paramyxoviridae. Two major surface antigens are expressed by both viruses; the highly conserved fusion (F protein, and the extremely diverse attachment (G glycoprotein. Both viruses comprise two genetic groups, A and B. Circulation frequencies of the two genetic groups fluctuate for both viruses, giving rise to frequently observed switching of the predominantly circulating group. Nucleotide sequence data for the F and G gene regions of HRSV and HMPV variants from the UK, The Netherlands, Bangkok and data available from Genbank were used to identify clades of both viruses. Several contemporary circulating clades of HRSV and HMPV were identified by phylogenetic reconstructions. The molecular epidemiology and evolutionary dynamics of clades were modelled in parallel. Times of origin were determined and positively selected sites were identified. Sustained circulation of contemporary clades of both viruses for decades and their global dissemination demonstrated that switching of the predominant genetic group did not arise through the emergence of novel lineages each respiratory season, but through the fluctuating circulation frequencies of pre-existing lineages which undergo proliferative and eclipse phases. An abundance of sites were identified as positively selected within the G protein but not the F protein of both viruses. For HRSV, these were discordant with previously identified residues under selection, suggesting the virus can evade immune responses by generating diversity at multiple sites within linear epitopes. For both viruses, different sites were identified as positively selected between genetic groups.

  16. Trypsin- and low pH-mediated fusogenicity of avian metapneumovirus fusion proteins is determined by residues at positions 100, 101 and 294.

    Science.gov (United States)

    Yun, Bingling; Guan, Xiaolu; Liu, Yongzhen; Gao, Yanni; Wang, Yongqiang; Qi, Xiaole; Cui, Hongyu; Liu, Changjun; Zhang, Yanping; Gao, Li; Li, Kai; Gao, Honglei; Gao, Yulong; Wang, Xiaomei

    2015-10-26

    Avian metapneumovirus (aMPV) and human metapneumovirus (hMPV) are members of the genus Metapneumovirus in the subfamily Pneumovirinae. Metapneumovirus fusion (F) protein mediates the fusion of host cells with the virus membrane for infection. Trypsin- and/or low pH-induced membrane fusion is a strain-dependent phenomenon for hMPV. Here, we demonstrated that three subtypes of aMPV (aMPV/A, aMPV/B, and aMPV/C) F proteins promoted cell-cell fusion in the absence of trypsin. Indeed, in the presence of trypsin, only aMPV/C F protein fusogenicity was enhanced. Mutagenesis of the amino acids at position 100 and/or 101, located at a putative cleavage region in aMPV F proteins, revealed that the trypsin-mediated fusogenicity of aMPV F proteins is regulated by the residues at positions 100 and 101. Moreover, we demonstrated that aMPV/A and aMPV/B F proteins mediated cell-cell fusion independent of low pH, whereas the aMPV/C F protein did not. Mutagenesis of the residue at position 294 in the aMPV/A, aMPV/B, and aMPV/C F proteins showed that 294G played a critical role in F protein-mediated fusion under low pH conditions. These findings on aMPV F protein-induced cell-cell fusion provide new insights into the molecular mechanisms underlying membrane fusion and pathogenesis of aMPV.

  17. Avian metapneumovirus in the USA

    Science.gov (United States)

    In the United States of America (USA), avian metapneumovirus (aMPV) causes an upper respiratory tract infection in turkeys; no outbreaks have been reported in commercial chicken flocks. Typical clinical signs of the disease in turkey poults include coughing, sneezing, nasal discharge, tracheal rale...

  18. Reverse genetics of avian metapneumoviruses

    Science.gov (United States)

    An overview of avian metapneumovirus (aMPV) infection in turkeys and development of a reverse genetics system for aMPV subgroup C (aMPV-C) virus will be presented. By using reverse genetics technology, we generated recombinant aMPV-C viruses containing a different length of glycoprotein (G) gene or...

  19. A Reverse Genetics Approach for the Design of Methyltransferase-Defective Live Attenuated Avian Metapneumovirus Vaccines.

    Science.gov (United States)

    Zhang, Yu; Sun, Jing; Wei, Yongwei; Li, Jianrong

    2016-01-01

    Avian metapneumovirus (aMPV), also known as avian pneumovirus or turkey rhinotracheitis virus, is the causative agent of turkey rhinotracheitis and is associated with swollen head syndrome in chickens. aMPV belongs to the family Paramyxoviridae which includes many important human pathogens such as human respiratory syncytial virus (RSV), human metapneumovirus (hMPV), and human parainfluenza virus type 3 (PIV3). The family also includes highly lethal emerging pathogens such as Nipah virus and Hendra virus, as well as agriculturally important viruses such as Newcastle disease virus (NDV). For many of these viruses, there is no effective vaccine. Here, we describe a reverse genetics approach to develop live attenuated aMPV vaccines by inhibiting the viral mRNA cap methyltransferase. The viral mRNA cap methyltransferase is an excellent target for the attenuation of paramyxoviruses because it plays essential roles in mRNA stability, efficient viral protein translation and innate immunity. We have described in detail the materials and methods used to generate recombinant aMPVs that lack viral mRNA cap methyltransferase activity. We have also provided methods to evaluate the genetic stability, pathogenesis, and immunogenicity of live aMPV vaccine candidates in turkeys.

  20. Human metapneumovirus: a new respiratory pathogen

    Indian Academy of Sciences (India)

    PRAKASH KUMAR

    the SH and G proteins (59 and 37% identity, respectively),. (van den Hoogen et al 2002; Biacchesi et al 2003; Herfst et al 2004) and is more than RSV subgroup A and B. hMPV. F protein, which is predicted to be the principal target of protective antibodies, is more conserved in hMPV strains than RSV. The overall level of ...

  1. A community study of clinical traits and risk factors for human metapneumovirus and respiratory syncytial virus infection during the first year of life

    DEFF Research Database (Denmark)

    von Linstow, Marie-Louise; Høgh, Mette; Nordbø, Svein

    2008-01-01

    Human metapneumovirus (hMPV) and respiratory syncytial virus (RSV) are important respiratory pathogens with similar symptomatology. The aim of this prospective birth cohort study was to identify risk factors for an hMPV or RSV infection during the first year of life in unselected healthy children....... We followed 217 children from birth to 1 year of age. Nasal swabs and symptom diaries were collected monthly. Anti-hMPV and anti-RSV IgG antibodies by age 1 year were detected by ELISA, and nasal swabs were analysed for hMPV and RSV by RT-PCR. Logistic regression was used for risk factor analysis....... Anti-hMPV IgG was found in 38 children (17.5%), and anti-RSV IgG in 172 children (79%). Risk factors for being anti-hMPV IgG-positive were: (1) being born in the spring (OR = 2.36; 95% CI:1.06-5.27), and (2) having older siblings (OR = 3.82; 95% CI:1.75-8.34). Risk factors for being anti-RSV Ig...

  2. Human Respiratory Syncytial Virus and Human Metapneumovirus

    OpenAIRE

    Luciana Helena Antoniassi da Silva; Fernando Rosado Spilki; Adriana Gut Lopes Riccetto; Emilio Elias Baracat; Clarice Weis Arns

    2009-01-01

    The human respiratory syncytial virus (hRSV) and the human metapneumovírus (hMPV) are main etiological agents of acute respiratory infections (ARI). The ARI is an important cause of childhood morbidity and mortality worldwide.  hRSV and hMPV are members of the Paramyxoviridae. They are enveloped, non-segmented viruses, with negative-sense single stranded genomes. Respiratory syncytial virus (hRSV) is the best characterized agent viral of this group, associated with respiratory diseases in...

  3. Isolation and characterization of a subtype C avian metapneumovirus circulating in Muscovy ducks in China.

    Science.gov (United States)

    Sun, Shikai; Chen, Feng; Cao, Sheng; Liu, Jiajia; Lei, Wen; Li, Guangwei; Song, Yongfeng; Lu, Junpeng; Liu, Chuang; Qin, Jianping; Li, Haiyan

    2014-07-25

    Subtype C avian metapneumovirus (aMPV-C), is an important pathogen that can cause egg-drop and acute respiratory diseases in poultry. To date, aMPV-C infection has not been documented in Muscovy ducks in China. Here, we isolated and characterized an aMPV-C, designated S-01, which has caused severe respiratory disease and noticeable egg drop in Muscovy duck flocks in south China since 2010. Electron microscopy showed that the isolate was an enveloped virus exhibiting multiple morphologies with a diameter of 20-500 nm. The S-01 strain was able to produce a typical cytopathic effect (CPE) on Vero cells and cause death in 10- to 11-day-old Muscovy duck embryos. In vivo infection of layer Muscovy ducks with the isolate resulted in typical clinical signs and pathological lesions similar to those seen in the original infected cases. We report the first complete genomic sequence of aMPV-C from Muscovy ducks. A phylogenetic analysis strongly suggested that the S-01 virus belongs to the aMPV-C family, sharing 92.3%-94.3% of nucleotide identity with that of aMPV-C, and was most closely related to the aMPV-C strains isolated from Muscovy ducks in France. The deduced eight main proteins (N, P, M, F, M2, SH, G and L) of the novel isolate shared higher identity with hMPV than with other aMPV (subtypes A, B and D). S-01 could bind a monoclonal antibody against the F protein of hMPV. Together, our results indicate that subtype-C aMPV has been circulating in Muscovy duck flocks in South China, and it is urgent for companies to develop new vaccines to control the spread of the virus in China.

  4. Metapneumovirus aviar: diagnóstico y control (Avian Metapneumovirus: diagnosis and control)

    OpenAIRE

    Acevedo Beiras, Ana María.

    2011-01-01

    ResumenEl Metapneumovirus aviar (aMPV) causa una infección aguda, altamente contagiosa del tracto respiratorio superior principalmente en pavos y pollos.SummaryAvian metapneumovirus (aMPV) causes an acute highly contagious upper respiratory tract infection primarily of turkeys and chickens.

  5. Zinc binding activity of human metapneumovirus M2-1 protein is indispensable for viral replication and pathogenesis in vivo.

    Science.gov (United States)

    Cai, Hui; Zhang, Yu; Ma, Yuanmei; Sun, Jing; Liang, Xueya; Li, Jianrong

    2015-06-01

    Human metapneumovirus (hMPV) is a member of the Pneumovirinae subfamily in the Paramyxoviridae family that causes respiratory tract infections in humans. Unlike members of the Paramyxovirinae subfamily, the polymerase complex of pneumoviruses requires an additional cofactor, the M2-1 protein, which functions as a transcriptional antitermination factor. The M2-1 protein was found to incorporate zinc ions, although the specific role(s) of the zinc binding activity in viral replication and pathogenesis remains unknown. In this study, we found that the third cysteine (C21) and the last histidine (H25) in the zinc binding motif (CCCH) of hMPV M2-1 were essential for zinc binding activity, whereas the first two cysteines (C7 and C15) play only minor or redundant roles in zinc binding. In addition, the zinc binding motif is essential for the oligomerization of M2-1. Subsequently, recombinant hMPVs (rhMPVs) carrying mutations in the zinc binding motif were recovered. Interestingly, rhMPV-C21S and -H25L mutants, which lacked zinc binding activity, had delayed replication in cell culture and were highly attenuated in cotton rats. In contrast, rhMPV-C7S and -C15S strains, which retained 60% of the zinc binding activity, replicated as efficiently as rhMPV in cotton rats. Importantly, rhMPVs that lacked zinc binding activity triggered high levels of neutralizing antibody and provided complete protection against challenge with rhMPV. Taken together, these results demonstrate that zinc binding activity is indispensable for viral replication and pathogenesis in vivo. These results also suggest that inhibition of zinc binding activity may serve as a novel approach to rationally attenuate hMPV and perhaps other pneumoviruses for vaccine purposes. The pneumoviruses include many important human and animal pathogens, such as human respiratory syncytial virus (hRSV), hMPV, bovine RSV, and avian metapneumovirus (aMPV). Among these viruses, hRSV and hMPV are the leading causes of acute

  6. Effect of amino acid sequence variations at position 149 on the fusogenic activity of the subtype B avian metapneumovirus fusion protein.

    Science.gov (United States)

    Yun, Bingling; Gao, Yanni; Liu, Yongzhen; Guan, Xiaolu; Wang, Yongqiang; Qi, Xiaole; Gao, Honglei; Liu, Changjun; Cui, Hongyu; Zhang, Yanping; Gao, Yulong; Wang, Xiaomei

    2015-10-01

    The entry of enveloped viruses into host cells requires the fusion of viral and cell membranes. These membrane fusion reactions are mediated by virus-encoded glycoproteins. In the case of avian metapneumovirus (aMPV), the fusion (F) protein alone can mediate virus entry and induce syncytium formation in vitro. To investigate the fusogenic activity of the aMPV F protein, we compared the fusogenic activities of three subtypes of aMPV F proteins using a TCSD50 assay developed in this study. Interestingly, we found that the F protein of aMPV subtype B (aMPV/B) strain VCO3/60616 (aMPV/vB) was hyperfusogenic when compared with F proteins of aMPV/B strain aMPV/f (aMPV/fB), aMPV subtype A (aMPV/A), and aMPV subtype C (aMPV/C). We then further demonstrated that the amino acid (aa) residue 149F contributed to the hyperfusogenic activity of the aMPV/vB F protein. Moreover, we revealed that residue 149F had no effect on the fusogenic activities of aMPV/A, aMPV/C, and human metapneumovirus (hMPV) F proteins. Collectively, we provide the first evidence that the amino acid at position 149 affects the fusogenic activity of the aMPV/B F protein, and our findings will provide new insights into the fusogenic mechanism of this protein.

  7. Identification of a truncated nucleoprotein in avian metapneumovirus-infected cells encoded by a second AUG, in-frame to the full-length gene

    Science.gov (United States)

    Alvarez, Rene; Seal, Bruce S

    2005-01-01

    Background Avian metapneumoviruses (aMPV) cause an upper respiratory disease with low mortality, but high morbidity primarily in commercial turkeys. There are three types of aMPV (A, B, C) of which the C type is found only in the United States. Viruses related to aMPV include human, bovine, ovine, and caprine respiratory syncytial viruses and pneumonia virus of mice, as well as the recently identified human metapneumovirus (hMPV). The aMPV and hMPV have become the type viruses of a new genus within the Metapneumovirus. The aMPV nucleoprotein (N) amino acid sequences of serotypes A, B, and C were aligned for comparative analysis. Based on predicted antigenicity of consensus protein sequences, five aMPV-specific N peptides were synthesized for development of peptide-antigens and antisera. Results The presence of two aMPV nucleoprotein (N) gene encoded polypeptides was detected in aMPV/C/US/Co and aMPV/A/UK/3b infected Vero cells. Nucleoprotein 1 (N1) encoded from the first open reading frame (ORF) was predicted to be 394 amino acids in length for aMPV/C/US/Co and 391 amino acids in length for aMPV/A/UK/3b with approximate molecular weights of 43.3 kilodaltons and 42.7 kilodaltons, respectively. Nucleoprotein 2 (N2) was hypothesized to be encoded by a second downstream ORF in-frame with ORF1 and encoded a protein predicted to contain 328 amino acids for aMPV/C/US/Co or 259 amino acids for aMPV/A/UK/3b with approximate molecular weights of 36 kilodaltons and 28.3 kilodaltons, respectively. Peptide antibodies to the N-terminal and C-terminal portions of the aMPV N protein confirmed presence of these products in both aMPV/C/US/Co- and aMPV/A/UK/3b-infected Vero cells. N1 and N2 for aMPV/C/US/Co ORFs were molecularly cloned and expressed in Vero cells utilizing eukaryotic expression vectors to confirm identity of the aMPV encoded proteins. Conclusion This is the first reported identification of potential, accessory in-frame N2 ORF gene products among members of the

  8. Identification of a truncated nucleoprotein in avian metapneumovirus-infected cells encoded by a second AUG, in-frame to the full-length gene

    Directory of Open Access Journals (Sweden)

    Alvarez Rene

    2005-04-01

    Full Text Available Abstract Background Avian metapneumoviruses (aMPV cause an upper respiratory disease with low mortality, but high morbidity primarily in commercial turkeys. There are three types of aMPV (A, B, C of which the C type is found only in the United States. Viruses related to aMPV include human, bovine, ovine, and caprine respiratory syncytial viruses and pneumonia virus of mice, as well as the recently identified human metapneumovirus (hMPV. The aMPV and hMPV have become the type viruses of a new genus within the Metapneumovirus. The aMPV nucleoprotein (N amino acid sequences of serotypes A, B, and C were aligned for comparative analysis. Based on predicted antigenicity of consensus protein sequences, five aMPV-specific N peptides were synthesized for development of peptide-antigens and antisera. Results The presence of two aMPV nucleoprotein (N gene encoded polypeptides was detected in aMPV/C/US/Co and aMPV/A/UK/3b infected Vero cells. Nucleoprotein 1 (N1 encoded from the first open reading frame (ORF was predicted to be 394 amino acids in length for aMPV/C/US/Co and 391 amino acids in length for aMPV/A/UK/3b with approximate molecular weights of 43.3 kilodaltons and 42.7 kilodaltons, respectively. Nucleoprotein 2 (N2 was hypothesized to be encoded by a second downstream ORF in-frame with ORF1 and encoded a protein predicted to contain 328 amino acids for aMPV/C/US/Co or 259 amino acids for aMPV/A/UK/3b with approximate molecular weights of 36 kilodaltons and 28.3 kilodaltons, respectively. Peptide antibodies to the N-terminal and C-terminal portions of the aMPV N protein confirmed presence of these products in both aMPV/C/US/Co- and aMPV/A/UK/3b-infected Vero cells. N1 and N2 for aMPV/C/US/Co ORFs were molecularly cloned and expressed in Vero cells utilizing eukaryotic expression vectors to confirm identity of the aMPV encoded proteins. Conclusion This is the first reported identification of potential, accessory in-frame N2 ORF gene products among

  9. Avian metapneumovirus subgroup C infection in chickens, China.

    Science.gov (United States)

    Wei, Li; Zhu, Shanshan; Yan, Xv; Wang, Jing; Zhang, Chunyan; Liu, Shuhang; She, Ruiping; Hu, Fengjiao; Quan, Rong; Liu, Jue

    2013-07-01

    Avian metapneumovirus causes acute respiratory tract infection and reductions in egg production in various avian species. We isolated and characterized an increasingly prevalent avian metapneumovirus subgroup C strain from meat-type commercial chickens with severe respiratory signs in China. Culling of infected flocks could lead to economic consequences.

  10. Avian Metapneumovirus Subgroup C Infection in Chickens, China

    OpenAIRE

    Wei, Li; Zhu, Shanshan; Yan, Xv; Wang, Jing; Zhang, Chunyan; Liu, Shuhang; She, Ruiping; Hu, Fengjiao; Quan, Rong; Liu, Jue

    2013-01-01

    Avian metapneumovirus causes acute respiratory tract infection and reductions in egg production in various avian species. We isolated and characterized an increasingly prevalent avian metapneumovirus subgroup C strain from meat-type commercial chickens with severe respiratory signs in China. Culling of infected flocks could lead to economic consequences.

  11. Avian Metapneumovirus Molecular Biology and Development of Genetically Engineered Vaccines

    Science.gov (United States)

    Avian metapneumovirus (aMPV) is an economically important pathogen of turkeys with a worldwide distribution. aMPV is a member of the genus Metapneumovirus within the subfamily Pneumovirinae of the family Paramyxoviridae. The genome of aMPV is a non-segmented, single-stranded, negative-sense RNA of 1...

  12. [Investigation of the presence of human metapneumovirus in patients with chronic obstructive pulmonary disease and asthma and its relationship with the attacks].

    Science.gov (United States)

    Ilvan, Ahmet; Aslan, Gönül; Serin, Mehmet Sami; Calıkoğlu, Mukadder; Yılmaz, Fatma Mehtap; Tezcan, Seda; Taş, Dilaver; Ayrık, Cüneyt; Uygungül, Evren; Sezer, Ogün; Emekdaş, Gürol

    2013-10-01

    Human metapneumovirus (hMPV), an enveloped RNA virus classified in Paramyxoviridae family, was first characterized in 2001 from children with acute respiratory tract infection. Recent studies have suggested hMPV to play a role in chronic obstructive pulmonary disease (COPD) and asthma attacks. The aims of this study were to investigate the frequency of hMPV in patients with COPD and asthma, its effects on the severity of the attacks and the relationship between demographical and clinical factors. A total of 123 patients, including 66 with COPD (45 were in attack and 21 were stable) and 57 with asthma (33 were in attack and 24 were under control) diagnosed according to the criteria of Global Initiative for Chronic Obstructive Lung Disease and the Global Strategy for Asthma Management and Prevention, respectively, were included in the study. Nasopharyngeal lavage samples collected from all of the patients have been evaluated for the presence of hMPV-RNA by using a reverse transcriptase-polymerase chain reaction (RT-PCR) targeting F gene region of the virus. hMPV-RNA positivity rates in patients with COPD and asthma were observed as 30.3% (20/66) and 31.6% (18/57), respectively, and the difference between the groups were not statistically significant (p= 1.00). When patients were compared according to their disease status, hMPV was detected in 31.1% (14/45) of patients with COPD attack and 28.6% of stable patients (p> 0.05). These rates were found as 36.4% (12/33) and 25% (6/24) in patients with asthma attack and controlled asthma, respectively (p> 0.05). Although the virus detection rates in patients with COPD and asthma attacks (26/78; 33.3%) were higher than the patients with stable/controlled disease (12/45; 26.7%), the difference was not found as statistically significant (p= 0.57). The detection rate of hMPV-RNA was 26.1% in patients who can be treated at home and hospital without any need of intensive care and mechanical ventilation, while this rate was 36

  13. Excretion patterns of human metapneumovirus and respiratory syncytial virus among young children

    DEFF Research Database (Denmark)

    von Linstow, Marie-Louise; Eugen-Olsen, Jesper; Koch, A

    2006-01-01

    in a three-week period. Sweat and blood samples were obtained at inclusion. The presence of RSV and hMPV RNA was detected using real-time RT-PCR. RESULTS: We detected RSV RNA in 28 saliva specimens, 5 stool samples, and 3 sweat samples. hMPV RNA was detected in one saliva specimen and two sweat samples. Four...

  14. Avian Metapneumovirus circulation in Italian broiler farms.

    Science.gov (United States)

    Tucciarone, Claudia Maria; Franzo, Giovanni; Lupini, Caterina; Alejo, Carolina Torres; Listorti, Valeria; Mescolini, Giulia; Brandão, Paulo Eduardo; Martini, Marco; Catelli, Elena; Cecchinato, Mattia

    2018-02-01

    With increasing frequency, avian Metapneumovirus (aMPV) is reported to induce respiratory signs in chickens. An adequate knowledge of current aMPV prevalence among Italian broilers is lacking, with little information available on its economical and health impact on the poultry industry. In order to collect preliminary data on the epidemiological context of aMPV in broiler flocks, a survey was performed in areas of Northern Italy with high poultry density from 2014 to 2016. Upper respiratory tract swabs were collected and processed by A and B subtype-specific multiplex real-time reverse transcription PCR (RT-PCR). Samples were also screened for infectious bronchitis virus (IBV) by generic RT-PCR and sequencing. Productive data and respiratory signs were detailed where possible. The high prevalence of aMPV was confirmed in broilers older than 26 d and also attested in IBV-negative farms. All aMPV detections belonged to subtype B. Italian strain genetic variability was evaluated by the partial attachment (G) gene sequencing of selected strains and compared with contemporary turkey strains and previously published aMPV references, revealing no host specificity and the progressive evolution of this virus in Italy. © 2017 Poultry Science Association Inc.

  15. El cuerpo humano

    OpenAIRE

    Tamir, Abraham; Ruiz Beviá, Francisco

    2015-01-01

    El cuerpo humano es la estructura completa del humano y comprende cabeza, cuello, tronco (que incluye el tórax y el abdomen), brazos y manos, piernas y pies. Cada parte del cuerpo se compone de varios tipos de células. En la madurez, el número medio estimado de células en el cuerpo es de 37.2 billones. La composición del cuerpo humano la constituyen un número de ciertos elementos químicos como Ca+2, Na+, K+, C, en diferentes proporciones. El estudio del cuerpo humano implica la anatomía (desc...

  16. Human Metapneumovirus and Respiratory Syncytial Virus Disease in Children, Yemen

    Science.gov (United States)

    Al-Sonboli, Najla; Hart, Charles A.; Al-Aghbari, Nasher; Al-Ansi, Ahmed; Ashoor, Omar

    2006-01-01

    Factors increasing the severity of respiratory infections in developing countries are poorly described. We report factors associated with severe acute respiratory illness in Yemeni children (266 infected with respiratory syncytial virus and 66 with human metapneumovirus). Age, indoor air pollution, and incomplete vaccinations were risk factors and differed from those in industrialized countries. PMID:17073098

  17. Complete nucleotide sequences of avian metapneumovirus subtype B genome.

    Science.gov (United States)

    Sugiyama, Miki; Ito, Hiroshi; Hata, Yusuke; Ono, Eriko; Ito, Toshihiro

    2010-12-01

    Complete nucleotide sequences were determined for subtype B avian metapneumovirus (aMPV), the attenuated vaccine strain VCO3/50 and its parental pathogenic strain VCO3/60616. The genomes of both strains comprised 13,508 nucleotides (nt), with a 42-nt leader at the 3'-end and a 46-nt trailer at the 5'-end. The genome contains eight genes in the order 3'-N-P-M-F-M2-SH-G-L-5', which is the same order shown in the other metapneumoviruses. The genes are flanked on either side by conserved transcriptional start and stop signals and have intergenic sequences varying in length from 1 to 88 nt. Comparison of nt and predicted amino acid (aa) sequences of VCO3/60616 with those of other metapneumoviruses revealed higher homology with aMPV subtype A virus than with other metapneumoviruses. A total of 18 nt and 10 deduced aa differences were seen between the strains, and one or a combination of several differences could be associated with attenuation of VCO3/50.

  18. Derechos humanos y prensa

    Directory of Open Access Journals (Sweden)

    Jorge Acevedo Rojas

    2015-01-01

    Full Text Available Pese a que la sociedad peruana en la década de los 90 se convirtió en dura cuestionadora de la violación de los derechos humanos, según estimaciones de organismos de defensa de derechos humanos, la violencia política causada en el Perú cobró más de 28 mil muertes, a pesar de la derrota de Sendero Luminoso. El artículo cuenta el problema, la reactualización de este, la contradicción flagrante, la percepción de los derechos humanos, los avances y retrocesos.

  19. A case series on common cold to severe bronchiolitis and pneumonia in children following human metapneumovirus infection in Sri Lanka.

    Science.gov (United States)

    Jayaweera, J A A S; Noordeen, F; Kothalaweala, S; Pitchai, F N N; Rayes, M L M

    2018-02-14

    The prevalence of hMPV infections in Sri Lanka has not been reported and here we report a case series of hMPV infection in children less than 5 years. Patients with ARTI were included from Teaching Hospital, Anuradhapura from March 2013 to August 2014. Indirect fluorescence assay was performed on nasopharyngeal aspirates for the identification of respiratory viruses [respiratory syncytial virus (RSV), parainfluenza virus 1, 2 and 3, influenza A and B and hMPV]. Moreover, reverse transcriptase-polymerase chain reaction was done to further confirm the hMPV infection. In this case series, hMPV infection showed a range of respiratory symptoms from common cold to life threatening lower respiratory tract infections with varying severity. In some cases, the clinical presentation of hMPV infection was similar to the ARTI caused by RSV. hMPV co-infections with of RSV have also been seen in some cases of ARTI. A child delivered through cesarean section and birth order > 3 has an Odds ratio of 3.5 and 4.3 (95% CI) for developing co-infection with RSV compared to hMPV mono-infections. Lack of diagnostic facilities to identify the viral aetiology has contributed to the use of antibiotics indicating the need for establishing viral diagnostic facilities in the country.

  20. Rational design of avian metapneumovirus live attenuated vaccines by inhibiting viral messenger RNA cap methyltransferase

    Science.gov (United States)

    Avian metapneumovirus (aMPV), also known as avian pneumovirus or turkey rhinotracheitis, is a non-segmented negative-sense RNA virus belonging to the family of Paramyxoviridae, the subfamily Pneumovirinae, and the genus Metapneumovirus. aMPV is the causative agent of respiratory tract infection and ...

  1. Derechos humanos y empresas

    OpenAIRE

    Domínguez Martín, Lidia

    2017-01-01

    Sólo a partir de finales del siglo XX, las empresas se han empezando a concienciar de la incidencia que puede tener su actuación en los derechos humanos y de la necesidad de integrar enfoques de responsabilidad social empresarial, no sólo por razones éticas, sino porque beneficia a los negocios y a la sociedad en general. Sin embargo, todavía son muchas las empresas que siguen vulnerando derechos humanos y, por ello, se han ido desarrollando una serie de iniciativas para garantizar que los re...

  2. Valores e Desenvolvimento Humano

    NARCIS (Netherlands)

    F. Comim (Flavio); A. Macedo de Jesus (Anderson); R.C.B Oliveira (Raissa); A. Davison (Anna); S. Galeno (Sabrina); A.C.V. Ribeiro (Ana)

    2010-01-01

    markdownabstractA primeira parte desse Relatório de Desenvolvimento Humano do Brasil 2009/2010 começa com a descrição de um amplo processo de consulta aberta à sociedade, denominada Brasil Ponto a Ponto, para a escolha do tema do relatório. A Campanha Brasil Ponto a Ponto teve por objetivo

  3. Biochemical characterization of the small hydrophobic protein of avian metapneumovirus.

    Science.gov (United States)

    Deng, Qiji; Song, Minxun; Demers, Andrew; Weng, Yuejin; Lu, Wuxun; Wang, Dan; Kaushik, Radhey S; Yu, Qingzhong; Li, Feng

    2012-08-01

    Avian metapneumovirus (AMPV) is a paramyxovirus that has three membrane proteins (G, F, and SH). Among them, the SH protein is a small type II integral membrane protein that is incorporated into virions and is only present in certain paramyxoviruses. In the present study, we show that the AMPV SH protein is modified by N-linked glycans and can be released into the extracellular environment. Furthermore, we demonstrate that glycosylated AMPV SH proteins form homodimers through cysteine-mediated disulfide bonds, which has not been reported previously for SH proteins of paramyxoviruses. Copyright © 2012 Elsevier B.V. All rights reserved.

  4. Microsporidios en humanos

    Directory of Open Access Journals (Sweden)

    Ligia Moncada

    1998-09-01

    Full Text Available Se hace una revisión sobre la taxonomía, la biología y los métodos diagnósticos de los microsporidios encontrados en humanos. A partir de 1981 -1982, con el advenimiento de la epidemia de SIDA, han sido muchos los informes sobre casos de microsporidiosis en humanos. Actualmente se han implicado especies correspondientes a los géneros Enterocytozoon, Encephalitozoon, Vittaforma, Pleistophora y Trachipleistophora, en diferentes patologías humanas no sólo en aquellos individuos que tienen algún compromiso del sistema inmune sino también en individuos sanos. Los métodos diagnósticos empleados se basan principalmente en coloraciones de cromotropo modificada y otras técnicas como microscopía electrónica de transmisión (TEM, análisis inmunogénicos y de ácidos nucleicos. La forma de transmisión para los humanos es incierta, pero se han encontrado algunas especies parasitando animales lo que sugiere que puede tratarse de una zoonosis.

  5. Contratos de capital humano

    OpenAIRE

    Oscar Alejandro Goyes Viteri

    2005-01-01

    Este documento busca dar a conocer el tema de la inversión en capital humano a través de la celebración de contratos atípicos y utilizando la fiducia mercantil y la titularización como vehículos financieros. Por medio de estos contratos un estudiante obtiene los recursos que requiere para financiar su educación superior, a cambio de entregar un porcentaje de sus ingresos después de su graduación a quien financió la operación.

  6. Contratos de capital humano

    Directory of Open Access Journals (Sweden)

    Oscar Alejandro Goyes Viteri

    2005-01-01

    Full Text Available Este documento busca dar a conocer el tema de la inversión en capital humano a través de la celebración de contratos atípicos y utilizando la fiducia mercantil y la titularización como vehículos financieros. Por medio de estos contratos un estudiante obtiene los recursos que requiere para financiar su educación superior, a cambio de entregar un porcentaje de sus ingresos después de su graduación a quien financió la operación.

  7. Contratos de capital humano

    Directory of Open Access Journals (Sweden)

    Oscar Alejandro Goyes Viteri

    2005-12-01

    Full Text Available Este documento busca dar a conocer el tema de la inversión en capital humano a través de la celebración de contratos atípicos y utilizando la fiducia mercantil y la titularización como vehículos financieros. Por medio de estos contratos un estudiante obtiene los recursos que requiere para financiar su educación superior, a cambio de entregar un porcentaje de sus ingresos después de su graduación a quien financió la operación.

  8. Los Derechos Humanos

    OpenAIRE

    Blanco Quirós, Miguel

    2014-01-01

    Faustino Cordón nos habla del derecho fundamental del científico: "Encontrar en su medio social cauces practicables, que recojan el pensamiento que el vayaganando en su pesquisa de la realidad y que lo conduzcan donde se contraste libremente con el pensamiento general". Para Ramón Tamames, desde el punto devista económico, la Declaración de los Derechos Humanos es una codificación internacional de validez jurídica muy discutida, que resume todas las conquistassociales de los trabajadores en l...

  9. Justicia y derechos humanos

    OpenAIRE

    Joel Flores Rentería

    2011-01-01

    Las libertades básicas y los bienes que contemplan los derechos humanos constituyen los principios de la justicia social y política en los Estados modernos, estos bienes son producto de la evolución histórica, política, cultural y filosófica de la modernidad. Por tal motivo, llevan en sí una conceptualización de lo que es y debe ser la existencia humana; en consecuencia, lo justo sería que toda persona, en una sociedad, se encuentre en posesión de dichos bienes y libertades.

  10. Field avian metapneumovirus evolution avoiding vaccine induced immunity.

    Science.gov (United States)

    Catelli, Elena; Lupini, Caterina; Cecchinato, Mattia; Ricchizzi, Enrico; Brown, Paul; Naylor, Clive J

    2010-01-22

    Live avian metapneumovirus (AMPV) vaccines have largely brought turkey rhinotracheitis (TRT) under control in Europe but unexplained outbreaks still occur. Italian AMPV longitudinal farm studies showed that subtype B AMPVs were frequently detected in turkeys some considerable period after subtype B vaccination. Sequencing showed these to be unrelated to the previously applied vaccine. Sequencing of the entire genome of a typical later isolate showed numerous SH and G protein gene differences when compared to both a 1987 Italian field isolate and the vaccine in common use. Experimental challenge of vaccinated birds with recent virus showed that protection was inferior to that seen after challenge with the earlier 1987 isolate. Field virus had changed in key antigenic regions allowing replication and leading to disease in well vaccinated birds.

  11. Complete Genome Sequence of an Avian Metapneumovirus Subtype A Strain Isolated from Chicken (Gallus gallus) in Brazil

    OpenAIRE

    Rizotto, La?s S.; Scagion, Guilherme P.; Cardoso, Tereza C.; Sim?o, Raphael M.; Caserta, Leonardo C.; Benassi, Julia C.; Keid, Lara B.; Oliveira, Tr?cia M. F. de S.; Soares, Rodrigo M.; Arns, Clarice W.; Van Borm, Steven; Ferreira, Helena L.

    2017-01-01

    ABSTRACT We report here the complete genome sequence of an avian metapneumovirus (aMPV) isolated from a tracheal tissue sample of a commercial layer flock. The complete genome sequence of aMPV-A/chicken/Brazil-SP/669/2003 was obtained using MiSeq (Illumina, Inc.) sequencing. Phylogenetic analysis of the complete genome classified the isolate as avian metapneumovirus subtype A.

  12. Generation of recombinant avian metapneumovirus subgroup C (aMPV-C) viruses containing different length of the G gene.

    Science.gov (United States)

    Genetic variation in length of the G gene among different avian metapneumovirus subgroup C isolates has been reported. However, its biological significance in virus replication, pathogenicity and immunity is unknown. In this study, we developed a reverse genetics system for avian metapneumovirus C a...

  13. VITIMOLOGIA E DIREITOS HUMANOS

    OpenAIRE

    Silva, João Felipe; Faculdades Integradas de Ourinhos. Professor de Ciência Política e Teoria Geral do Estado. Nome do grupo: DEMOCRACIA E DIREITOS FUNDAMENTAIS Status do grupo: certificado pela instituição Ano de formação: 2012 Data da última atualização: 18/04/2013 11:42 Líder(es) do grupo: Fernando de Brito Alves - Edinilson Donisete Machado - Área predominante: Ciências Sociais Aplicadas; Direito Instituição: Universidade Estadual do Norte do Paraná - UENP Órgão: Setor de Pós-Graduação em Direito

    2013-01-01

    As questões atinentes à situação da vítima e seu comportamento frente ao algoz e as formas como se dá a vitimização em seus diversos graus apresenta-se de forma inquietante desde o período imediatamente posterior à Segunda Guerra Mundial. O surgimento da Vitimologia como área do conhecimento, cujo principal objetivo é o resguardo dos Direitos Humanos da vítima concretiza e afirma deforma categórica o quão importante para as ciências penais e criminológicas é a percepção dos aspectos biológico...

  14. de los derechos humanos

    Directory of Open Access Journals (Sweden)

    Javier Pardo Falcón

    2007-01-01

    Full Text Available A pesar de su uso en determinados foros políticos con connotaciones claramente negativas, el término globalización tiene un significado neutro que también puede hacer referencia a fines de bondad indudable. Así, tanto la Declaración Universal de los Derechos Humanos como otros instrumentos internacionales aprobados por las Naciones Unidas son normas jurídicas globalizadoras, surgidas con la idea de extender la vigencia de los derechos básicos de la persona a todos los países del mundo. Desde esta otra visión de la globalización, conseguir la efectiva vigencia de los derechos humanos en los países de Centroamérica resulta una tarea particularmente urgente y necesaria. En efecto, salvo Costa Rica, que puede considerarse una afortunada excepción en la turbulenta historia política centroamericana, todos los demás Estados de la región comparten un pasado marcado por los graves conflictos internos y el escaso desarrollo económico, circunstancias ambas que explican en buena parte un presente caracterizado por la debilidad de las instituciones, la falta de cohesión social y, en consecuencia, la insuficiente garantía de los derechos más básicos de la mayoría de la población. En este contexto, y desde su compromiso con la mejora de las condiciones materiales de vida de sus conciudadanos menos favorecidos, seis autores y autoras centroamericanos abordan, desde distintas perspectivas, la situación actual de los derechos fundamentales en sus respectivos países.

  15. Topology and cellular localization of the small hydrophobic protein of avian metapneumovirus.

    Science.gov (United States)

    Deng, Qiji; Weng, Yuejin; Lu, Wuxun; Demers, Andrew; Song, Minxun; Wang, Dan; Yu, Qingzhong; Li, Feng

    2011-09-01

    The small hydrophobic protein (SH) is a type II integral membrane protein that is packaged into virions and is only present in certain paramyxoviruses including metapneumovirus. In addition to a highly divergent primary sequence, SH proteins vary significantly in size amongst the different viruses. Human respiratory syncytial virus (HRSV) encodes the smallest SH protein consisting of only 64 amino acids, while metapneumoviruses have the longest SH protein ranging from 174 to 179 amino acids in length. Little is currently known about the cellular localization and topology of the metapneumovirus SH protein. Here we characterize for the first time metapneumovirus SH protein with respect to topology, subcellular localization, and transport using avian metapneumovirus subgroup C (AMPV-C) as a model system. We show that AMPV-C SH is an integral membrane protein with N(in)C(out) orientation located in both the plasma membrane as well as within intracellular compartments, which is similar to what has been described previously for SH proteins of other paramyxoviruses. Furthermore, we demonstrate that AMPV-C SH protein localizes in the endoplasmic reticulum (ER), Golgi, and cell surface, and is transported through ER-Golgi secretory pathway. Copyright © 2011 Elsevier B.V. All rights reserved.

  16. Profesores universitarios y derechos humanos

    OpenAIRE

    Peralta-Varela, Carlos; Pérez-Viramontes, Gerardo

    1997-01-01

    Se presentan algunas reflexiones sobre la forma como los textos internacionales entienden la relación educación-derechos humanos, a partir de la idea de la educación como el instrumento más importante para conformar una cultura universal de respeto a los derechos humanos. Se presenta la situación que guardan la educación y los derechos humanos en América Latina y en particular en México, así como el papel que se les asigna a los maestros para hacer que los valores universales se reafirmen en ...

  17. Nucleotide and Predicted Amino Acid Sequence-Based Analysis of the Avian Metapneumovirus Type C Cell Attachment Glycoprotein Gene: Phylogenetic Analysis and Molecular Epidemiology of U.S. Pneumoviruses

    Science.gov (United States)

    Alvarez, Rene; Lwamba, Humphrey M.; Kapczynski, Darrell R.; Njenga, M. Kariuki; Seal, Bruce S.

    2003-01-01

    A serologically distinct avian metapneumovirus (aMPV) was isolated in the United States after an outbreak of turkey rhinotracheitis (TRT) in February 1997. The newly recognized U.S. virus was subsequently demonstrated to be genetically distinct from European subtypes and was designated aMPV serotype C (aMPV/C). We have determined the nucleotide sequence of the gene encoding the cell attachment glycoprotein (G) of aMPV/C (Colorado strain and three Minnesota isolates) and predicted amino acid sequence by sequencing cloned cDNAs synthesized from intracellular RNA of aMPV/C-infected cells. The nucleotide sequence comprised 1,321 nucleotides with only one predicted open reading frame encoding a protein of 435 amino acids, with a predicted Mr of 48,840. The structural characteristics of the predicted G protein of aMPV/C were similar to those of the human respiratory syncytial virus (hRSV) attachment G protein, including two mucin-like regions (heparin-binding domains) flanking both sides of a CX3C chemokine motif present in a conserved hydrophobic pocket. Comparison of the deduced G-protein amino acid sequence of aMPV/C with those of aMPV serotypes A, B, and D, as well as hRSV revealed overall predicted amino acid sequence identities ranging from 4 to 16.5%, suggesting a distant relationship. However, G-protein sequence identities ranged from 72 to 97% when aMPV/C was compared to other members within the aMPV/C subtype or 21% for the recently identified human MPV (hMPV) G protein. Ratios of nonsynonymous to synonymous nucleotide changes were greater than one in the G gene when comparing the more recent Minnesota isolates to the original Colorado isolate. Epidemiologically, this indicates positive selection among U.S. isolates since the first outbreak of TRT in the United States. PMID:12682171

  18. El genoma humano

    Directory of Open Access Journals (Sweden)

    García Barreno, Pedro

    2002-01-01

    Full Text Available Not available

    El redescubrimiento de las leyes de Mendel sobre la herencia en las semanas que abrieron el siglo XX incitó una búsqueda científica para comprender la naturaleza y el contenido de la información genética que ha impulsado la biología durante los últimos cien años. El progreso científico conseguido se ha fraguado en cuatro fases que se corresponden, aproximadamente, con los cuatro cuartos del siglo XX. El primero estableció las bases celulares de la herencia: los cromosomas. El segundo definió las bases moleculares de la herencia: la doble hélice de ADN. El tercero descifró las bases informativas de la herencia con el descubrimiento de los mecanismos biológicos mediante los que la célula lee la información codificada en los genes; luego, con la invención de la tecnología del ADN recombinante de clonajey de secuenciación, los científicos pudieron hacer lo mismo. El último cuarto del siglo estuvo marcado por un lento pero constante esfuerzo para descifrar genes primero y, por fin, genomas enteros que han propiciado el desarrollo de la genómica. El día 26 de junio de 2000 se hacía público un «borrador de trabajo» de la secuencia del genoma humano. Las revistas Nature (vol 409, n.º 6822 y Science (vol 291, n.º 5507 dedicaban números especiales a la publicación de la secuencia en el mes de febrero de 2001 (el día 15 Nature y, al día siguiente, Science. «La humanidad ha recibido un gran regalo. La conclusión de la secuencia del genoma humano ofrece una herramienta poderosa para descifrar los secretos de nuestra herencia genética y para precisar nuestro lugar entre otros participantes en la aventura de la vida».

  19. LINGUAGEM, INTERSUBJETIVIDADE E MOVIMENTO HUMANO

    Directory of Open Access Journals (Sweden)

    Lísia Costa Gonçalves de Araújo

    2005-01-01

    Full Text Available Este estudo aborda o tema do Movimento Humano, num contexto interdisciplinar, com base na ontologia da Linguagem em Merleau-Ponty e na teoria do agir comunicativo em Habermas. A Linguagem é tema central no processo educacional. Está intimamente ligada ao Movimento Humano, pois este cria um dinamismo, através da própria percepção que lhe é inerente, abrindo-nos para novas possibilidades de “ser no mundo”.

  20. Brazilian avian metapneumovirus subtypes A and B: experimental infection of broilers and evaluation of vaccine efficacy

    OpenAIRE

    Márcia B. dos Santos; Matheus C. Martini; Helena L. Ferreira; Luciana H.A. da Silva; Paulo A. Fellipe; Fernando R. Spilki; Clarice W. Arns

    2012-01-01

    Santos M.B., Martini M.C., Ferreira H.L., Silva L.H.A., Fellipe P.A., Spilki F.R. & Arns C.W. 2012. Brazilian avian metapneumovirus subtypes A and B: experimental infection of broilers and evaluation of vaccine efficacy. Pesquisa Veterinaria Brasileira 32(12):1257-1262. Laboratorio de Virologia, Instituto de Biologia, Universidade Estadual de Campinas, Rua Monteiro Lobato s/n, Cx. Postal 6109, Campinas, SP 13083-970, Brazil. E-mail: Avian metapneumovirus (aMPV) is a respirator...

  1. Detection and subtyping avian metapneumovirus from turkeys in Iran.

    Science.gov (United States)

    Mayahi, Mansour; Momtaz, Hassan; Jafari, Ramezan Ali; Zamani, Pejman

    2017-01-01

    Avian metapneumovirus (aMPV) causes diseases like rhinotracheitis in turkeys, swollen head syndrome in chickens and avian rhinotracheitis in other birds. Causing respiratory problems, aMPV adversely affects production and inflicts immense economic losses and mortalities, especially in turkey flocks. In recent years, several serological and molecular studies have been conducted on this virus, especially in poultry in Asia and Iran. The purpose of the present study was detecting and subtyping aMPV by reverse transcriptase polymerase chain reaction (RT-PCR) from non-vaccinated, commercial turkey flocks in Iran for the first time. Sixty three meat-type unvaccinated turkey flocks from several provinces of Iran were sampled in major turkey abattoirs. Samples were tested by RT-PCR for detecting and subtyping aMPV. The results showed that 26 samples from three flocks (4.10%) were positive for viral RNA and all of the viruses were found to be subtype B of aMPV. As a result, vaccination especially against subtype B of aMPV should be considered in turkey flocks in Iran to control aMPV infections.

  2. Molecular detection and isolation of avian metapneumovirus in Mexico.

    Science.gov (United States)

    Rivera-Benitez, José Francisco; Martínez-Bautista, Rebeca; Ríos-Cambre, Francisco; Ramírez-Mendoza, Humberto

    2014-01-01

    We conducted a longitudinal study to detect and isolate avian metapneumovirus (aMPV) in two highly productive poultry areas in Mexico. A total of 968 breeder hens and pullets from 2 to 73 weeks of age were analysed. Serology was performed to detect aMPV antibodies and 105 samples of tracheal tissue were collected, pooled by age, and used for attempted virus isolation and aMPV nested reverse transcriptase-polymerase chain reaction (nRT-PCR). The serological analysis indicated that 100% of the sampled chickens showed aMPV antibodies by 12 weeks of age. Five pools of pullet samples collected at 3 to 8 weeks of age were positive by nRT-PCR and the sequences obtained indicated 98 to 99% similarity with the reported sequences for aMPV subtype A. Virus isolation of nRT-PCR-positive samples was successfully attempted using chicken embryo lung and trachea mixed cultures with subsequent adaptation to Vero cells. This is the first report of detection and isolation of aMPV in Mexico.

  3. Isolation and characterization of avian metapneumovirus from chickens in Korea.

    Science.gov (United States)

    Kwon, Ji-Sun; Lee, Hyun-Jeong; Jeong, Seung-Hwan; Park, Jeong-Yong; Hong, Young-Ho; Lee, Youn-Jeong; Youn, Ho-Sik; Lee, Dong-Woo; Do, Sun-Hee; Park, Seung-Yong; Choi, In-Soo; Lee, Joong-Bok; Song, Chang-Seon

    2010-03-01

    Avian metapneumovirus (aMPV) causes upper respiratory tract infections in chickens and turkeys. Although the swollen head syndrome (SHS) associated with aMPV in chickens has been reported in Korea since 1992, this is the study isolating aMPV from chickens in this country. We examined 780 oropharyngeal swab or nasal turbinate samples collected from 130 chicken flocks to investigate the prevalence of aMPV and to isolate aMPV from chickens from 2004-2008. Twelve aMPV subtype A and 13 subtype B strains were detected from clinical samples by the aMPV subtype A and B multiplex real-time reverse transcription polymerase chain reaction (RRT-PCR). Partial sequence analysis of the G glycoprotein gene confirmed that the detected aMPVs belonged to subtypes A and B. Two aMPVs subtype A out of the 25 detected aMPVs were isolated by Vero cell passage. In animal experiments with an aMPV isolate, viral RNA was detected in nasal discharge, although no clinical signs of SHS were observed in chickens. In contrast to chickens, turkeys showed severe nasal discharge and a relatively higher titer of viral excretion than chickens. Here, we reveal the co-circulation of aMPV subtypes A and B, and isolate aMPVs from chicken flocks in Korea.

  4. Topology and cellular localization of the small hydrophobic protein of avian metapneumovirus

    Science.gov (United States)

    The small hydrophobic protein (SH) is a type II integral membrane protein that is packaged into virions and is only present in certain paramyxoviruses including metapneumovirus. In addition to a highly divergent primary sequence, SH proteins vary significantly in size among the different viruses. Hu...

  5. Development of Recombinant Newcastle Disease Viruses Expressing the Glycoprotein (G) of Avian Metapneumovirus as Bivalent Vaccines

    Science.gov (United States)

    Using reverse genetics technology, Newcastle disease virus (NDV) LaSota strain-based recombinant viruses were engineered to express the glycoprotein (G) of avian metapneumovirus (aMPV), subtype A, B or C, as bivalent vaccines. These recombinant viruses were slightly attenuated in vivo, yet maintaine...

  6. Limited inter- and intra-patient sequence diversity of the genetic lineage a human metapneumovirus fusion gene

    DEFF Research Database (Denmark)

    Winther, T.N.; Madsen, C.D.; Pedersen, Anders Gorm

    2005-01-01

    been infected with at least two viruses. Several independent viruses contained premature stop codons in exactly identical positions resulting in truncated fusion proteins. Possibly this is a mechanism for immune system evasion. The F protein is a major antigenic determinant, and the limited sequence....... In this study, the inter- and intra-patient genetic diversity of the lineage A hMPV F gene was investigated. Ten isolates were collected from 10 hMPV infected children. Viral RNA was isolated and amplified, and approximately 10 clones from each isolate were sequenced. Altogether 108 clones were successfully...

  7. Derechos Humanos y Derecho Internacional

    Directory of Open Access Journals (Sweden)

    Carrillo Salcedo, Juan Antonio

    2000-09-01

    Full Text Available Not available

    La aparición de un nuevo consenso internacional en torno a las nociones de derechos humanos y democracia, como valores generalmente aceptados por la comunidad de naciones en su conjunto, y la afirmación de que el ser humano es titular de derechos propios, oponibles jurídicamente a todos los Estados, constituyen extraordinarias innovaciones que hacen que, a diferencia del Derecho internacional clásico, la persona no pueda seguir siendo considerada como un mero objeto del Derecho internacional.

  8. Sistema prisional e direitos humanos

    OpenAIRE

    Mendes, André Pacheco Teixeira

    2015-01-01

    O volume 4 da Série Clínicas dos Cadernos FGV DIREITO RIO traz quatro trabalhos originais relacionados ao tema do Sistema Prisional e Direitos Humanos. A obra contempla pareceres jurídicos sobre violações aos direitos dos presos nas unidades prisionais do estado do Rio de Janeiro, tendo por objeto: (i) emprego ilegal de laxante em custodiados e visitantes suspeitos de carregarem drogas; (ii) falta de água própria para o consumo humano; (iii) restrição ao banho de sol; e (iv) condições inadequ...

  9. Emociones, justicia y derechos humanos

    OpenAIRE

    Baum, Erica

    2011-01-01

    En el contexto del mundo globalizado actual, en el que proliferan los movimientos sociales de indignación por las injusticias y el horror, esta tesis presenta una perspectiva en derechos humanos sobre el rol de las emociones en la justicia, especialmente en el fuero criminal, y sobre el limite que establecen a dicha relación los principios éticos de igual valor y dignidad humana, autonomía personal y diversidad cultural, en que se fundan los derechos humanos. Siguiendo la teoría neo-estoica d...

  10. Myasis Miasis en humanos

    Directory of Open Access Journals (Sweden)

    Rafael Valderrama Hernández

    1991-02-01

    ón con larvas de mosca produce diversas manifestaciones según el sitio afectado y puede, Incluso, causar la muerte. No es una enfermedad común en humanos pero se observa con alguna regularidad en Colombia y en otros países neotropicales. Afecta con mayor frecuencia las áreas expuestas de la piel y se presenta raramente en ojos, nariz, senos paranasales, tracto urogenital o recto; en estos casos la Infestación se asocia con traumas previos o secreciones purulentas que atraen a las moscas adultas. La carencia de registros y de bibliografía adecuados no permite evaluar el Impacto real de esta parasitosis. Con la presente revisión se pretende contribuir a su conocimiento.

  11. Complete Genome Sequence of an Avian Metapneumovirus Subtype A Strain Isolated from Chicken (Gallus gallus) in Brazil.

    Science.gov (United States)

    Rizotto, Laís S; Scagion, Guilherme P; Cardoso, Tereza C; Simão, Raphael M; Caserta, Leonardo C; Benassi, Julia C; Keid, Lara B; Oliveira, Trícia M F de S; Soares, Rodrigo M; Arns, Clarice W; Van Borm, Steven; Ferreira, Helena L

    2017-07-20

    We report here the complete genome sequence of an avian metapneumovirus (aMPV) isolated from a tracheal tissue sample of a commercial layer flock. The complete genome sequence of aMPV-A/chicken/Brazil-SP/669/2003 was obtained using MiSeq (Illumina, Inc.) sequencing. Phylogenetic analysis of the complete genome classified the isolate as avian metapneumovirus subtype A. Copyright © 2017 Rizotto et al.

  12. Characterization of the Fusion and Attachment Glycoproteins of Human Metapneumovirus and Human Serosurvey to Determine Reinfection Rates

    Science.gov (United States)

    2007-06-27

    infections (30). Rhinorrhea, fever and cough are the most frequently reported clinical findings (75). Otitis media , conjunctivitis and pharyngitis are...primates immunized with hMPV develop antibodies reactive to both the monomeric and trimeric sF glycoproteins, and animals immunized with the same genotype...ELISA).............................................34 Animal immunization

  13. Avian metapneumovirus subtype C in Wild Waterfowl in Ontario, Canada.

    Science.gov (United States)

    Jardine, C M; Parmley, E J; Buchanan, T; Nituch, L; Ojkic, D

    2018-02-18

    Avian metapneumovirus (aMPV) is an emerging poultry pathogen that has a significant economic impact on poultry production worldwide. The geographic range of the virus continues to expand, and wild birds have been implicated as reservoirs of aMPV that have the potential to spread the virus over long distances. Our objective was to determine the apparent prevalence of aMPV subtype C in wild waterfowl in Ontario, Canada. Wild waterfowl were captured in August and September, 2016 as part of routine migratory waterfowl population monitoring by the Ontario Ministry of Natural Resources and Forestry. Oropharyngeal and cloacal swabs were collected from each bird and placed together for aMPV testing using real-time RT-PCR. A total of 374 live wild birds from 23 lakes were sampled and tested for aMPV. Among all ducks tested, 84 (22%) were positive for aMPV. The proportion of samples that tested positive ranged from 0% in ring-necked ducks (Aythya collaris) and green-winged teal (Anas carolinensis) to 44% (8 of 18) in American black ducks (A. rubripes). Waterfowl positive for aMPV were found at 14 of 23 lakes in the study area and the percent positive at these 14 lakes ranged between 5% and 84%. Although subtype C aMPV has been detected in a variety of wild birds in North America, this is the first report of aMPV in wild ducks in Ontario, Canada. The high apparent prevalence, particularly in mallards and American black ducks (37 and 44%, respectively), suggests that these species may be important reservoirs of aMPV. Given the potential impact of aMPV on domestic poultry and the potential role of wild birds as reservoirs of the virus, further investigation of the geographic distribution, risk factors associated with aMPV carriage in wild waterfowl and potential role of other birds in the epidemiology of aMPV in Canada is warranted. © 2018 Blackwell Verlag GmbH.

  14. Naturaleza de los derechos humanos

    Directory of Open Access Journals (Sweden)

    Carlos López Dawson

    2016-07-01

    Full Text Available En la formación de una nueva Carta Fundamental los constituyentes requerirán conocer o consensuar sobre la naturaleza de los derechos humanos; luego, determinar cómo el Estado hará efectivo el respeto de tales derechos. Para ello es necesario recurrir a la historia y evolución de estos derechos, y el presente trabajo trata de contribuir a un debate productivo eficiente sobre la naturaleza de los derechos humanos, para que los ciudadanos puedan decidir en el entendido de que se trata de una decisión democrática reflexionada y fundada en el humanismo. El análisis se sitúa en el contexto técnico-ideológico del sistema republicano vigente que corresponde al estado actual del derecho internacional.

  15. Universalidad de los Derechos Humanos

    Directory of Open Access Journals (Sweden)

    Orlando José Cadrazco Salcedo

    2011-07-01

    Full Text Available Resumen:La dimensión universal de los Derechos Humanos ha sido proclamada reiteradamente en el sistema de las Naciones Unidas. Dicha universalidad pretende reflejar y, a su vez, fortalecer el consenso de la comunidad internacional respecto de dichos derechos, sin que ello tenga que suponer, en principio, la imposición de ningún tipo de hegemonía jurídica, política o cultural, en particular la occidental, euro céntrica o atlantista. Sin embargo, ya sabemos que esto no resulta nada fácil ante la diversidad cultural de nuestro mundo. Por ejemplo, a nosotros, los “occidentales”, ahora nos resultan especialmente chocantes las normas -jurídicas o morales- fuertemente discriminatorias contra las mujeresexistentes en la mayoría de las culturas y religiones.Palabras claves:Derechos Humanos, Universalidad, Estados Sociales de Derecho, Positivización, Seres Humanos, Occidente, Organización de Naciones Unidas.Abstract :The universal dimension of human rights has been proclaimed repeatedly in the United Nations system. This universality is intended to reflect and, in turn, strengthen the international community consensus on these rights, without having to assume, in principle, the imposition of hegemony any legal, political or cultural, including Western central euro or Atlanta. However, we know that it is not easy to cultural diversity of our world. For example, we as “Westerners”, now we are particularly shocking and legal rules or moral, highly discriminatory against women exist in most cultures and religions.Key words:Human Rights, Universality, Social State of Law, Positivization, Human Beings, West, United Nations Organization.

  16. El virus del papiloma humano

    OpenAIRE

    Cabrero Díaz, Sara

    2012-01-01

    La infección por el Virus del Papiloma Humano es en nuestros tiempos una de las enfermedades de transmisión sexual más frecuentes y su prevalencia puede alcanzar hasta el 60% en las personas activas sexualmente. Este trabajo prentende recopilar datos sobre la enfermedad para comprender el por qué de su transmisión (causas), cómo se manifiesta (síntomas), complicaciones que éste puede producir, técnicas que se usan para su diagnóstico, el posible tratamiento y la controversia que éste ha ca...

  17. Justicia, emociones y derechos humanos

    OpenAIRE

    Baum, Erica

    2011-01-01

    Los conflictos humanos llevados a la justicia poseen un componente emocional irreductible: sentimos ira ante el daño, temor ante una amenaza y vergüenza ante una humillación. Las emociones morales, por otro lado, se distinguen de las emociones básicas que experimentan otras especies animales no humanas por su contenido evaluativo y cognitivo. Interactúan con la razón, revelándonos el estado de vulnerabilidad que nos es inherente. Hay una larguísima discusión filosófica sobre el papel de dicha...

  18. Creencias y virus papiloma humano

    OpenAIRE

    Concha P,Ximena; Urrutia S,Teresa; Riquelme H,Giselle

    2012-01-01

    Antecedentes: La infección por Virus Papiloma Humano (VPH) es una enfermedad de transmisión sexual, que afecta a un alto porcentaje de mujeres jóvenes de todo el mundo. Su alta incidencia está asociada al inicio precoz y mayor frecuencia de la actividad sexual. A pesar de que la infección por VPH está ampliamente difundida en el mundo, es una infección aún desconocida por la población, lo que asociado a diversos factores culturales, favorece el desarrollo de múltiples creencias que dificultan...

  19. Population dynamics and rates of molecular evolution of a recently emerged paramyxovirus, avian metapneumovirus subtype C.

    Science.gov (United States)

    Padhi, Abinash; Poss, Mary

    2009-02-01

    We report the existence of two distinct sublineages of avian metapneumovirus (MPV) subtype C, a virus which has caused serious economic loss in commercial turkey farms in the United States. This subtype is closely related to human MPV, infects multiple avian species, and is globally distributed. The evolutionary rates of this virus are estimated to be 1.3 x 10(-3) to 7 x 10(-3) substitutions per site per year, and coalescent estimates place its emergence between 1991 and 1996. The four genes examined show a concordant demographic pattern which is characterized by a rapid increase in population size followed by stable population grown until the present.

  20. The Role of Bokavirus and Metapneumovirus in Development Acute Respiratory Infections in Hospitalized Patients in 2011—2014 years in Penza Region

    Directory of Open Access Journals (Sweden)

    I. A. Malova

    2016-01-01

    Full Text Available The article presents the results of a comparative analysis of the clinical data, laboratory and instrumental examination methods in patients with bokavirus (HBoV and metapnevmovirus (HMPV infections, hospitalized in the infectious disease Department of MSU № 59 Zarechnyj sity Penza region. From 713 patients with verified diagnosis with 42 (5.89% PCR revealed metapnevmovirus infection, at 18 (2.52% bokavirus, at 34 (4.77% mixed infection, most often by HBoV on and HMPV were children up to 7 years, were accompanied by lesions of the upper and lower respiratory tract infections, fever, intoxication, adenopathy.

  1. Production of monoclonal antibodies for Avian Metapneumovirus (SHS-BR-121 isolated in Brazil

    Directory of Open Access Journals (Sweden)

    LT Coswig

    2007-12-01

    Full Text Available Avian Metapneumovirus (aMPV, also called Turkey Rhinotracheitis Virus (TRTV, is an upper respiratory tract infection of turkeys, chickens and other avian species. Five monoclonal antibodies (MAbs were created against the Brazilian isolate (SHS-BR-121 of aMPV, MAbs 1A5B8; 1C1C4; 2C2E9 and 2A4C3 of IgG1 and MAb 1C1F8 of IgG2a. Four Mabs (1A5B8; 1C1C4; 2C2E9 and 2A4C3 showed neutralizing activity and three (1A5B8; 1C1C4 and 2A4C3 inhibited cellular fusion in vitro. These MAbs were used to investigate antigenic relationship among three strains (SHS-BR-121, STG 854/88 and TRT 1439/91 of aMPV subtypes A and B using cross-neutralization test. The results confirm that the monoclonal antibodies described can be used as a valuable tool in the epizootiological and serological studies, and also for the specific diagnosis of the subtypes in the infection for Avian Metapneumovirus.

  2. Avian metapneumovirus subtype A in China and subtypes A and B in Nigeria.

    Science.gov (United States)

    Owoade, A A; Ducatez, M F; Hübschen, J M; Sausy, A; Chen, H; Guan, Y; Muller, C P

    2008-09-01

    In order to detect and characterize avian metapneumovirus, organs or swabs were collected from 697 chicken and 110 turkeys from commercial farms in Southwestern Nigeria and from 107 chickens from live bird markets in Southeastern China. In Nigeria, 15% and 6% of the chicken and turkey samples, respectively, and 39% of the chicken samples from China, were positive for aMPV genome by PCR. The sequence of a 400 nt fragment of the attachment protein gene (G gene) revealed the presence of aMPV subtype A in both Nigeria and Southeastern China. Essentially identical subtype A viruses were found in both countries and were also previously reported from Brazil and the United Kingdom, suggesting a link between these countries or a common source of this subtype. In Nigeria, subtype B was also found, which may be a reflection of chicken importations from most major poultry-producing countries in Europe and Asia. In order to justify countermeasures, further studies are warranted to better understand the metapneumoviruses and their impact on poultry production.

  3. Interacciones humano-animal: características e implicaciones para el bienestar de los humanos

    OpenAIRE

    Natalia Piar; Diana Granados; Germán Gutiérrez

    2007-01-01

    En este artículo se revisa la literatura reciente sobre interacciones humano-animal, su origen, sus características y cómo dichas interacciones afectan el bienestar físico, psicológico y social. En la primera parte se presentan los orígenes y evolución histórica de las relaciones humano - animal, empezando por los procesos de domesticación y se aborda la naturaleza de las interacciones humano animal. En la segunda parte, se revisan los efectos para los humanos de ...

  4. A Wild Goose Metapneumovirus Containing a Large Attachment Glycoprotein Is Avirulent but Immunoprotective in Domestic Turkeys

    Science.gov (United States)

    Bennett, Richard S.; LaRue, Rebecca; Shaw, Daniel; Yu, Qingzhong; Nagaraja, K. V.; Halvorson, David A.; Njenga, M. Kariuki

    2005-01-01

    The genomic structure and composition of an avian metapneumovirus (aMPV) recently isolated from wild Canada geese (goose 15a/01) in the United States, together with its replication, virulence, and immunogenicity in domestic turkeys, were investigated. The sizes of seven of the eight genes, sequence identity, and genome organization of goose aMPV were similar to those of turkey aMPV subtype C (aMPV/C) strains, indicating that it belonged to the subtype. However, the goose virus contained the largest attachment (G) gene of any pneumovirus or metapneumovirus, with the predicted G protein of 585 amino acids (aa) more than twice the sizes of G proteins from other subtype C viruses and human metapneumovirus and more than 170 aa larger than the G proteins from the other aMPV subtypes (subtypes A, B, and D). The large G gene resulted from a 1,015-nucleotide insertion at 18 nucleotides upstream of the termination signal of the turkey aMPV/C G gene. Three other aMPV isolates from Canada geese had similarly large G genes, whereas analysis of recent aMPV strains circulating in U.S. turkeys did not indicate the presence of the goose virus-like strain. In vitro, the goose virus replicated to levels (2 × 105 to 5 × 105 50% tissue culture infective dose) comparable to those produced by turkey aMPV/C strains. More importantly, the virus replicated efficiently in the upper respiratory tract of domestic turkeys but with no clinical signs in either day-old or 2-week-old turkeys. The virus was also horizontally transmitted to naïve birds, and turkey infections with goose 15a/01 induced production of aMPV-specific antibodies. Challenging day-old or 2-week-old turkeys vaccinated with live goose aMPV resulted in lower clinical scores in 33% of the birds, whereas the rest of the birds had no detectable clinical signs of the upper respiratory disease, suggesting that the mutant virus may be a safe and effective vaccine against aMPV infection outbreaks in commercial turkeys. PMID:16282483

  5. Ensaio sobre desenvolvimento humano na pós-modernidade

    OpenAIRE

    Abib,José Antônio Damásio

    2008-01-01

    Neste ensaio, o conceito de desenvolvimento humano na pós-modernidade apóia-se em uma filosofia pós-moderna de direitos humanos, e na modernidade apóia-se em uma filosofia moderna de direitos humanos. O Estado moderno fracassou na realização da filosofia moderna dos direitos humanos. Uma filosofia pós-moderna de direitos humanos deixa para trás a filosofia moderna de direitos humanos. Isso significa abandonar o fundacionismo ético e as grandes narrativas emancipatórias de desenvolvimento huma...

  6. First Identification and Molecular Characterization of Avian metapneumovirus Subtype B from Chickens in Greece.

    Science.gov (United States)

    Tucciarone, Claudia Maria; Andreopoulou, Marianna; Franzo, Giovanni; Prentza, Zoi; Chaligiannis, Ilias; Cecchinato, Mattia

    2017-09-01

    Avian metapneumovirus (aMPV) is considered a major pathogen for turkeys but its impact on chicken production is still partially neglected, even though it is fully acknowledged as a primary pathogen in chickens as well. The lack of structured diagnostic surveys does not allow a pervasive understanding of aMPV epidemiology. Being that aMPV is almost an everyday challenge for farmers and veterinarians, a more accurate report of its presence should be detailed, posing the basis for a deep and global epidemiologic analysis. With these premises, the present work aims to report the first detection and molecular characterization of aMPV subtype B field strains from unvaccinated chickens in Greece. The Greek strains appear to be phylogenetically related among each other and with other recent Mediterranean strains while being distant from the currently applied vaccines, thus stressing once more the necessity to evaluate aMPV diffusion and evolution.

  7. Hatchability, serology and virus excretion following in ovo vaccination of chickens with an avian metapneumovirus vaccine.

    Science.gov (United States)

    Hess, M; Huggins, M B; Heincz, U

    2004-12-01

    The present investigation describes for the first time the effect of an avian metapneumovirus vaccine administered in ovo to 18-day-old chicken embryos. The application of the vaccine had no adverse effect on the hatchability or the health of the chicks post hatch. The antibody titres achieved were higher than those determined for birds vaccinated at 1 day old. Not only were the mean titres in the in ovo vaccinated groups higher, but many more birds developed a measurable antibody response than birds vaccinated at 1 day old. Variation of the vaccine dose used in ovo had little effect on the serological responses that peaked 21 to 28 days post hatch. Re-isolation of the vaccine virus was much more successful from birds vaccinated in ovo than from birds vaccinated at 1 day old, and detection of the nucleic acid by polymerase chain reaction correlated with the results of live virus isolation.

  8. A turkey rhinotracheitis outbreak caused by the environmental spread of a vaccine-derived avian metapneumovirus.

    Science.gov (United States)

    Lupini, Caterina; Cecchinato, Mattia; Ricchizzi, Enrico; Naylor, Clive J; Catelli, Elena

    2011-10-01

    Avian metapneumovirus (aMPV) subtype A was isolated from 7-week-old turkeys showing respiratory disease typical of turkey rhinotracheitis. Comparison of the virus sequence with previously determined vaccine marker sequences showed that the virulent virus had originated from a licensed live subtype A aMPV vaccine. The vaccine had neither been in use on the farm within a period of at least 6 months nor had it been used on farms within a distance of approximately 5 km. Isolation of the virus and exposure to naive turkeys caused disease typical of a virulent aMPV field strain. The study shows that disease was caused by exposure to aMPV vaccine-derived virus that was present in the environment, and indicates that such virus is able to circulate for longer than was previously envisaged.

  9. Development of a vaccine-challenge model for avian metapneumovirus subtype C in turkeys.

    Science.gov (United States)

    Velayudhan, Binu T; Noll, Sally L; Thachil, Anil J; Shaw, Daniel P; Goyal, Sagar M; Halvorson, David A; Nagaraja, Kakambi V

    2007-02-26

    The objective of this study was to evaluate different preparations of avian metapneumovirus (aMPV) subtype C as vaccine challenge in turkeys. Two aMPV isolates and their respective nasal turbinate homogenates after propagation in turkeys were used in the study. Significantly higher clinical sign scores were recorded in birds inoculated with 20 or 2% turbinate homogenate of recent isolate. Birds in the above groups showed more pronounced histopathological lesions, and a higher percentage of birds showed viral RNA and antigen in tissues. The data demonstrated that nasal turbinate homogenate of recent isolate produced severe clinical signs and lesions in turkeys and could be an ideal candidate for vaccine-challenge studies.

  10. Production of monoclonal antibodies for Avian Metapneumovirus (SHS-BR-121) isolated in Brazil

    OpenAIRE

    Coswig,LT; Stach-Machado,DR; Arns,CW

    2007-01-01

    Avian Metapneumovirus (aMPV), also called Turkey Rhinotracheitis Virus (TRTV), is an upper respiratory tract infection of turkeys, chickens and other avian species. Five monoclonal antibodies (MAbs) were created against the Brazilian isolate (SHS-BR-121) of aMPV, MAbs 1A5B8; 1C1C4; 2C2E9 and 2A4C3 of IgG1 and MAb 1C1F8 of IgG2a. Four Mabs (1A5B8; 1C1C4; 2C2E9 and 2A4C3) showed neutralizing activity and three (1A5B8; 1C1C4 and 2A4C3) inhibited cellular fusion in vitro. These MAbs were used to ...

  11. Contribution of the attachment G glycoprotein to pathogenicity and immunogenicity of avian metapneumovirus subgroup C.

    Science.gov (United States)

    Govindarajan, Dhanasekaran; Kim, Shin-Hee; Samal, Siba K

    2010-03-01

    Avian metapneumovirus (AMPV) causes an upper respiratory tract infection in turkeys leading to serious economic losses to the turkey industry. The G glycoprotein of AMPV is known to be associated with viral attachment and pathogenesis. In this study, we determined the role of the G glycoprotein in the pathogenicity and immunogenicity of AMPV strain Colorado (AMPV/CO). Recombinant AMPV/CO lacking the G protein (rAMPV/CO-deltaG) was generated using a reverse-genetics system. The recovered rAMPV/CO-deltaG replicated slightly better than did wild-type AMPV in Vero cells. However, deletion of the G gene in AMPV resulted in attenuation of the virus in turkeys. The mutant virus induced less-severe clinical signs and a weaker immune response in turkeys than did the wild-type AMPV. Our results suggest that the G glycoprotein is an important determinant for the pathogenicity and immunogenicity of AMPV.

  12. Efficacy of gamithromycin against Ornithobacterium rhinotracheale in turkey poults pre-infected with avian metapneumovirus.

    Science.gov (United States)

    Watteyn, Anneleen; Devreese, Mathias; Plessers, Elke; Wyns, Heidi; Garmyn, An; Reddy, Vishwanatha R A P; Pasmans, Frank; Martel, An; Haesebrouck, Freddy; De Backer, Patrick; Croubels, Siska

    2016-10-01

    Ornithobacterium rhinotracheale is an avian respiratory pathogen that affects turkeys. The objective of this study was to evaluate the clinical efficacy of gamithromycin (GAM) against O. rhinotracheale in turkeys. The birds were inoculated oculonasally with 10(8) colony-forming units (cfu) of O. rhinotracheale, preceded by infection with avian metapneumovirus. In addition to a negative (CONTR-) and a positive control group (CONTR+) there were two treated groups administered GAM (6 mg/kg) either subcutaneously (GAM SC) or orally (GAM PO) by administration as a single bolus at one-day post-bacterial infection (p.b.i.). From the start of the avian metapneumovirus infection until the end of the experiment, the turkeys were examined clinically and scored daily. In addition, tracheal swabs were collected at several days p.b.i. Necropsy was performed at 4, 8 and 12 days p.b.i. to evaluate the presence of gross lesions, and to collect trachea and lung tissue samples and air sac swabs for O. rhinotracheale quantification. The clinical score of the GAM SC group showed slightly lower values and birds recovered earlier than those in the GAM PO and CONTR+ groups. O. rhinotracheale cfus were significantly reduced in tracheal swabs of the SC group between 2 and 4 days p.b.i. At necropsy, CONTR+ showed higher O. rhinotracheale cfu in lung tissues compared to the treated groups. Moreover, at 8 days p.b.i. only the lung samples of CONTR+ were positive. In conclusion, the efficacy of GAM against O. rhinotracheale was demonstrated, especially in the lung tissue. However, the PO bolus administration of the commercially available product was not as efficacious as the SC bolus.

  13. Por que administrar estrategicamente recursos humanos?

    OpenAIRE

    Almeida,Martinho Isnard Ribeiro de; Teixeira,Maria Luiza Mendes; Martinelli,Dante Pinheiro

    1993-01-01

    Este artigo aborda a necessidade de se dar um tratamento estratatégico à Administração de Recursos Humanos, da mesma forma como normalmente ocorre em outras áreas da Administração, de maneira que as Organizações possam estabelecer um relacionamento positivo e duradouro com o seu ambiente. O artigo sugere, de forma prática, algumas atividades de Recursos Humanos que deverão estar presentes nas diferentes etapas de um plano estratégico. This text deals with the necessity of a strategic treat...

  14. Los derechos humanos en el deporte

    OpenAIRE

    Kidd, Bruce

    2004-01-01

    Los derechos humanos en el deporte se refieren a un mandato genérico que abarca una serie de derechos relacionados entre sí. La base del movimiento a favor de los derechos radica en aceptar el concepto fundamental de que el deporte y el tiempo libre son un derecho de todos los seres humanos, necesario para su desarrollo, su salud y para el bienestar tanto de los individuos como de la sociedad. Los deportistas han luchado por el derecho a ser tratados con justicia y dignidad en todos los aspec...

  15. Globalización y desarrollo humano

    OpenAIRE

    Unceta Satrustegui, Koldo

    1999-01-01

    Este trabajo analiza las relaciones existentes entre la creciente globalización de las actividades económicas y las posibilidades de desarrollo humano. Para ello, se realizan en primer término algunas precisiones conceptuales sobre el fenómeno de la globalización, distinguiendo entre los aspectos cuantitativos y cualitativos de la misma. A continuación se plantean las premisas de la ampliación de las capacidades y las libertades como características básicas del desarrollo humano, y las limita...

  16. Os direitos humanos e seus paradoxos

    OpenAIRE

    Proner, Caroline

    2001-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Jurídicas. Programa de Pós-Graduação em Direito Como resultado final dessa investigação, que pretendeu confrontar, mediante análise procedimental, os limites e avanços do sistema americano de proteção de direitos humanos com a realidade do paradoxo fundamental, algumas considerações podem ser apresentadas : o sistema americano de proteção de direitos humanos ; o suposto consenso da universalização dos direi...

  17. Interacciones humano-animal: características e implicaciones para el bienestar de los humanos

    Directory of Open Access Journals (Sweden)

    Natalia Piar

    2007-07-01

    Full Text Available En este artículo se revisa la literatura reciente sobre interacciones humano-animal, su origen, sus características y cómo dichas interacciones afectan el bienestar físico, psicológico y social. En la primera parte se presentan los orígenes y evolución histórica de las relaciones humano - animal, empezando por los procesos de domesticación y se aborda la naturaleza de las interacciones humano animal. En la segunda parte, se revisan los efectos para los humanos de la interacción con animales, especialmente con sus mascotas y se analiza la literatura científica sobre los efectos físicos, psicológicos y sociales. Finalmente, se aborda brevemente el desarrollo de esta área de investigación en Latinoamérica.

  18. EPIDEMIOLOGIA DEL VIRUS PAPILOMA HUMANO (HPV)

    OpenAIRE

    Rivera Z.,René; Aguilera T.,Jorge; Larraín H,Angélica

    2002-01-01

    Se presenta una revisión de la epidemiología del Virus Papiloma Humano (HPV). Patogenia, prevalencia mundial, magnitud de la infección, factores de riesgo, algunas lesiones producidas según tipo específico y se describe el concepto de clearance y persistencia viral.

  19. Los incómodos derechos humanos

    OpenAIRE

    Kesch, Emilie

    2014-01-01

    A casi sesenta y un años de la firma del tratado por la ONU sobre los Derechos Humanos son innumerables los asesinatos, genocidios, y torturas ocurridos en todo el mundo. Basta con ojear un :poco en nuestro pasado histórico para darnos cuenta que la teoría difiere abismalmente de la práctica.

  20. Desarrollo Humano Sustentable: Concepto y Naturaleza

    OpenAIRE

    Fajardo Arturo, Luis Andrés

    2006-01-01

    El concepto de Desarrollo Humano Sustentable; implica una conjunción de elementos económicos; jurídicos; filosóficos y antropológicos que hacen difícil un concepto sintético. Ese problema se hace aún más complejo a la hora de determinar si se trata de un concepto de naturaleza jurídica que pueda ser catalogado como un Derecho Humano; o si más bien se trata de una pretensión política. Sin embargo; el desarrollo doctrinal sobre el tema deja augurar la existencia de un derecho; al menos de exist...

  1. Comparative evaluation of conventional RT-PCR and real-time RT-PCR (RRT-PCR) for detection of avian metapneumovirus subtype A

    OpenAIRE

    Ferreira, HL; Spilki, FR; dos Santos, MMAB; de Almeida, RS; Arns, CW

    2009-01-01

    Avian metapneumovirus (AMPV) belongs to Metapneumovirus genus of Paramyxoviridae family. Virus isolation, serology, and detection of genomic RNA are used as diagnostic methods for AMPV. The aim of the present study was to compare the detection of six subgroup A AMPV isolates (AMPV/A) viral RNA by using different conventional and real time RT-PCR methods. Two new RT-PCR tests and two real time RT-PCR tests, both detecting fusion (F) gene and nucleocapsid (N) gene were compared with an establis...

  2. EMPREENDEDORISMO E VALORES HUMANOS: UM ESTUDO CONCEITUAL

    Directory of Open Access Journals (Sweden)

    Luciano Vignochi

    2013-09-01

    Full Text Available O objetivo principal deste artigo foi identificar conceitos de valores humanos em publicações científicas sobre empreendedorismo. Não há um único conceito de valores humanos, gerando diversas abordagens e aplicações do termo em estudos sobre empreendedorismo. Para identificar diferenças conceituais e aplicações práticas, optou-se por realizar uma análise de conteúdo em um portfólio de artigos selecionados em bases de dados. O principal critério de seleção das publicações foi aderência ao tema. Os resultados mostram que valores podem ser definidos como: critérios de julgamento da realidade; aspectos abstratos, cognitivos e afetivos ou características determinantes da personalidade e do comportamento de empreendedores. Valores influenciam a sobrevivência do indivíduo, o crescimento do empreendimento e podem nivelar a congruência do empreendedor e seu propósito de empreendimento com os critérios de convivência social. Ainda foram discriminados resultados de estudos aplicados com vistas a destacar evidências de contribuições práticas de pesquisas sobre valores humanos em empreendedorismo. Futuras investigações para aprofundar e replicar as análises realizadas neste estudo podem contribuir com a formação de empreendedores, a realização de diagnóstico empresarial e com avanços para a consolidação um modelo de desenvolvimento de empresas baseado em valores humanos.

  3. El comportamiento humano y la accientalidad

    OpenAIRE

    Salas Ollé, Carles

    2016-01-01

    El comportamiento humano es un factor que afecta y es afec - tado por las actitudes de las personas, e influye de manera fun - damental en la aparición de los accidentes. por ello, el estudio de dicho comportamiento se convierte en objetivo prioritario para investigar la causalidad de los accidentes y, en consecuen - cia, para los profesionales que se dedican a la prevención de los mismos

  4. Justicia, propiedad intelectual y genoma humano

    Directory of Open Access Journals (Sweden)

    Maria Graciela De Ortuzar

    2014-06-01

    Full Text Available A partir del conocimiento de la secuenciación del genoma humano es posible el descubrimiento de nuevas terapias, drogas, y causas de enfermedades. Sin embargo, lejos de aumentar el acceso a la atención de la salud, el proyecto genoma humano restringió dicho acceso debido a la rápida comercialización y al creciente ?patentamiento del material humano? -hecho que atenta contra el fundamento ético del sistema jurídico internacional de patentes-.En manos exclusivas de monopolios privados, las ?patentes genéticas? pueden traducirse en altísimos costos de nuevas drogas, tests, y tratamientos para los países en desarrollo. Por lo tanto, si bien la salud humana es presentada como el objetivo último de las investigaciones genómicas, la citada comercialización atenta contra el derecho a la salud y el derecho al conocimiento debido a que imponen una mayor desigualdad y vulnerabilidad de los países en desarrollo por su restricción de acceso. Cabe destacar que es en dichos(países donde se obtiene la información primariaii. En éste nuevo orden internacional de distribución en salud impuesto desde la Organización Mundial de Comercio- OMC- y no desde la Organización Mundial de Salud - OMS-; el actor principal en la ley de patentes, y el beneficiario de la protección que la misma provee, no es ahora el que inventa sino el que invierte.iii Por ello, el objetivo general de la presente investigación es elaborar un marco ético-legal universalista, con especificación plural en ámbitos locales, que regule la investigación del genoma humano en pos de la justa distribución de sus beneficios .

  5. Homo floresiensis. Humanos distintos a nosotros.

    OpenAIRE

    Carlos Alberto Marmelada

    2008-01-01

    Desde su descubrimiento, la polémica en torno al estatus de Homo floresiensis no ha cesado. ¿Son humanos de nuestra propia especie, pero que eran pigmeos que padecieron enanismo, microcefalia y otra serie de patologías, o se trata de miembros de una especie distinta a la nuestra? El hallazgo de restos pertenecientes a 13 individuos y el estudio de varias partes de su esqueleto avalan la segunda hipótesis.

  6. Homo floresiensis. Humanos distintos a nosotros.

    Directory of Open Access Journals (Sweden)

    Carlos Alberto Marmelada

    2008-08-01

    Full Text Available Desde su descubrimiento, la polémica en torno al estatus de Homo floresiensis no ha cesado. ¿Son humanos de nuestra propia especie, pero que eran pigmeos que padecieron enanismo, microcefalia y otra serie de patologías, o se trata de miembros de una especie distinta a la nuestra? El hallazgo de restos pertenecientes a 13 individuos y el estudio de varias partes de su esqueleto avalan la segunda hipótesis.

  7. Duración y tiempo humano.

    OpenAIRE

    González-Umeres, L. (Luz)

    2002-01-01

    Este trabajo presenta dos visiones del tiempo humano: las de Bergson y Polo. La propuesta bergsoniana está fechada en París, 1888, en un ambiente mecanicista al cual se enfrenta, recuperando el tiempo psicológico. La propuesta de Polo iniciada a mediados del siglo XX va más allá del nivel psicológico: se adentra en el ámbito de los trascendentales personales.

  8. POBREZA, RECURSOS HUMANOS Y DESARROLLO SOSTENIBLE

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    Jorge Mora Alfaro

    2011-09-01

    Full Text Available Dos desafíos de enorme trascendencia enfrentan América Latina y el Caribe al arribar al siglo XXI: por un lado, crear las condiciones apropiadas para insertarse con éxito en el interconectado, competitivo y exigente mundo contemporáneo. Por otro lado, superar los obstáculos al logro de la aspiración del desarrollo humano sostenible, entre los que sobresale la ausencia de mecanismos eficaces de distribución del ingreso y la significativa cantidad de seres humanos sumidos en la pobreza y las privaciones.Estas dos dimensiones del desarrollo regional se encuentran estrechamente entrelazadas y mutuamente condicionadas. Una distribución más equitativa del ingreso contribuye a elevar la movilidad social, el acceso a la educación, el aumento de la productividad y la estabilidad, aspectos esenciales para acrecentar la capacidad competitiva y para lograr una inserción de calidad en el contexto internacional. Una estrategia de integración en el contexto internacional, sustentada en las fortalezas nacionales, en la identidad cultural y en el capital humano, posibilita la sostenibilidad y la distribución de los beneficios entre los diversos estratos de la sociedad.

  9. Flujos migratorios y movilidad del capital humano

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    Bernardí Cabrer

    2009-11-01

    Full Text Available En este trabajo se estiman los flujos migratorios de capital humano entre las regiones españolas a partir de un conjunto de hipótesis. Los resultados indican un aumento de la intensidad de los flujos brutos de migrantes con niveles de instrucción de 2° y 3er grado. Se constata que en España existen tres polos de atracción y emisión de capital humano centrados en Madrid, País Vasco y Cataluña, y actuando de forma independiente. Asimismo, se obtiene un grado de adecuación apreciable entre el nivel educativo de los inmigrantes y los requerimientos de cualificación de la mano de obra de cada región. Sin embargo, se obtiene que los años medios de estudio de la población potencialmente activa han aumentado frente a la estabilidad de los de la población migrante, por lo que no cabe señalar la inmigración como un factor relevante para reducir las disparidades interregionales en capital humano.

  10. Flujos migratorios y movilidad del capital humano

    Directory of Open Access Journals (Sweden)

    Bernardí Cabrer

    2009-01-01

    Full Text Available En este trabajo se estiman los flujos migratorios de capital humano entre las regiones españolas a partir de un conjunto de hipótesis. Los resultados indican un aumento de la intensidad de los flujos brutos de migrantes con niveles de instrucción de 2° y 3er grado. Se constata que en España existen tres polos de atracción y emisión de capital humano centrados en Madrid, País Vasco y Cataluña, y actuando de forma independiente. Asimismo, se obtiene un grado de adecuación apreciable entre el nivel educativo de los inmigrantes y los requerimientos de cualificación de la mano de obra de cada región. Sin embargo, se obtiene que los años medios de estudio de la población potencialmente activa han aumentado frente a la estabilidad de los de la población migrante, por lo que no cabe señalar la inmigración como un factor relevante para reducir las disparidades interregionales en capital humano.

  11. Obligaciones morales con seres no humanos

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    Rodrigo J. Ocampo

    2014-06-01

    Full Text Available Entre los modelos de reflexión ética a partir de los cuales es posible dar cuenta del debate sobre las relaciones entre seres humanos y no humanos están: el deontológico, el contractualista, el utilitarista, el neoaristotélico y el discursivo. En este artículo se examinará, a partir de ellos, la cuestión del estatus moral asignado a los animales y a la naturaleza, junto con la posibilidad de cada uno para sustentar la ampliación de las obligaciones morales. Para tal fin, se estudian los principales argumentos para reconocer o negar consideración moral a seres no humanos, así como los alcances de estas justificaciones para comprender el sentido y límites de la comunidad ética. Esto permitirá clasificar los enfoques éticos mencionados atendiendo a sus afinidades con diversas formas de antropocentrismo o biocentrismo.

  12. La época del talento humano

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    Mónica Murillo de Pisco

    2017-08-01

    Full Text Available "El  valor humano e  el  recurso más valioso  con  el  que  cuenta  toda organización", con  esta  frase  se inicia un comercial de televisión. el mismo  pretende promocionar  un seminario de Recursos Humanos en una de  las  principales ciudades del país,  la frase menciona lo que todos conocemos y palpamos  a diario en nuestros trabajos. pues la 'Adminis­ tración del Talento', es un tema que se  repite de manera permanente en las  conversaciones  de  los  profe­ sionales  de  RRHI 1,  y  de  forma rápida se convierte en el plato fuerte de  casi  todas  las  jornadas, seminarios  y  cursos  a  los  que asistimos:  el  Talento.  se  convierte entonces en  la  principal  herra­ mienta de todo buen administrador de  recursos  humanos  y  una estrategia primordial  para todas las organizaciones.

  13. The pathogenicity of avian metapneumovirus subtype C wild bird isolates in domestic turkeys

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    Cha Ra Mi

    2013-01-01

    Full Text Available Abstract Background Avian metapneumovirus subtype C (aMPV/C causes severe upper respiratory disease in turkeys. Previous report revealed the presence of aMPV/C in wild birds in the southeast regions of the U.S. Methods In this study, aMPV/C positive oral swabs from American coots (AC and Canada geese (CG were passaged three times in the respiratory tract of specific pathogen free (SPF turkeys and used as aMPV/C P3 virus isolates in subsequent studies. Results Wild bird P3 isolates showed similar growth characteristics when compared to virulent aMPV/C in chicken embryo fibroblast ( CEF cell cultures and their glycoprotein G gene sequence was closely related to the G gene of aMPV/C Colorado reference virus. Three-day-old commercial or SPF turkeys were inoculated oculonasally with wild bird aMPV/C P3 isolates. At 5 and 7 days post-inoculation (DPI, severe clinical signs were observed in both of the AC and CG virus-exposed groups. Viral RNA was detected in tracheal swabs by reverse transcriptase polymerase chain reaction (RT-PCR. In addition, immunohistochemistry showed virus replication in the nasal turbinate and trachea. All virus-exposed turkeys developed positive antibody response by 14 DPI. Conclusions Our data demonstrate that aMPV/C wild bird isolates induced typical aMPV/C disease in the domestic turkeys.

  14. Avian metapneumovirus excretion in vaccinated and non-vaccinated specified pathogen free laying chickens.

    Science.gov (United States)

    Hess, M; Huggins, M B; Mudzamiri, R; Heincz, U

    2004-02-01

    Vaccinated and non-vaccinated specified pathogen-free White Leghorn laying chickens were challenged at peak of lay by the intravenous or oculonasal route with a virulent avian metapneumovirus (aMPV) subtype B chicken strain. Severe clinical signs and a drop in egg production were induced in the non-vaccinated intravenously challenged birds whereas the vaccinates were not affected. Live virus excretion was demonstrated in the faeces and respiratory tract of non-vaccinated hens for up to 7 days post intravenous challenge. After oculonasal challenge, virus excretion could only be demonstrated in the respiratory tract for up to 5 days. No live virus excretion was found in either the faeces or the respiratory tract of vaccinated birds. Concurrent with live virus isolation, the presence of viral RNA was demonstrated by single reverse transcription-polymerase chain reaction (RT-PCR). Nested RT-PCR was more sensitive and viral RNA could be detected in non-vaccinated birds up to 28 days post either intravenous or oculonasal challenge, at which time the experiment was terminated. Viral RNA was detected for up to 12 days in vaccinated birds. This is the first study investigating excretion of aMPV and viral RNA in vaccinated and non-vaccinated laying hens challenged under experimental conditions. The results are of importance with regard to the persistence of aMPV and the appropriate diagnostic detection method in laying birds.

  15. Detection of avian metapneumovirus subtypes in turkeys using RT-PCR.

    Science.gov (United States)

    Ongor, H; Karahan, M; Kalin, R; Bulut, H; Cetinkaya, B

    2010-03-20

    This study investigated the prevalence of avian metapneumovirus (aMPV) and the detection of molecular subtypes of field strains of the virus using RT-PCR in clinically healthy turkeys and those showing signs of respiratory disease. In the RT-PCR examination of 624 tracheal tissue samples collected from a local turkey abattoir, 2.9 per cent (18/624) of samples tested positive. In the examination of tracheal swab samples collected from flocks with respiratory problems, 18 of 20 samples tested positive. When the results were assessed at flock level, aMPV infection was detected in only one of the 23 clinically healthy turkey flocks, whereas all four flocks with respiratory problems were infected. Molecular typing using primers specific to the attachment glycoprotein (G) gene showed that all 36 positive samples belonged to subtype B. Partial sequence analysis of DNA samples showed 95 per cent homology between the field types and the reference strain aMPV subtype B. Whereas clinically healthy turkeys had been vaccinated with a subtype A virus vaccine, the flocks with respiratory problems had been vaccinated with a subtype B virus vaccine. Despite four blind passages of RT-PCR-positive samples on Vero and chicken embryo fibroblast cells, no cytopathic effect was detected by microscopic examination.

  16. Immunization with avian metapneumovirus harboring chicken Fc induces higher immune responses.

    Science.gov (United States)

    Paudel, Sarita; Easwaran, Maheswaran; Jang, Hyun; Jung, Ho-Kyoung; Kim, Joo-Hun; Shin, Hyun-Jin

    2016-07-15

    In this study, we evaluated the immune responses of avian metapneumovirus harboring chicken Fc molecule. Stable Vero cells expressing chicken Fc chimera on its surface (Vero-cFc) were established, and we confirmed that aMPV grown in Vero-cFc incorporated host derived chimera Fc into the aMPV virions. Immunization of chicken with aMPV-cFc induced higher level of antibodies and inflammatory cytokines; (Interferon (IFN)-γ and Interleukin (IL)-1β) compared to those of aMPV. The increased levels of antibodies and inflammatory cytokines in chicken immunized with aMPV-cFc were statistically significantly (p<0.05) to that of aMPV and control. The aMPV-cFc group also generated the highest neutralizing antibody response. After challenges, chickens immunized with aMPV-cFc showed much less pathological signs in nasal turbinates and trachea so that we could confirm aMPV-cFc induced higher protection than that of aMPV. The greater ability of aMPV harboring chicken Fc to that of aMPV presented it as a possible vaccine candidate. Copyright © 2016 Elsevier B.V. All rights reserved.

  17. Brazilian avian metapneumovirus subtypes A and B: experimental infection of broilers and evaluation of vaccine efficacy

    Directory of Open Access Journals (Sweden)

    Márcia B. dos Santos

    2012-12-01

    Full Text Available Avian metapneumovirus (aMPV is a respiratory pathogen associated with the swollen head syndrome (SHS in chickens. In Brazil, live aMPV vaccines are currently used, but subtypes A and, mainly subtype B (aMPV/A and aMPV/B are still circulating. This study was conducted to characterize two Brazilian aMPV isolates (A and B subtypes of chicken origin. A challenge trial to explore the replication ability of the Brazilian subtypes A and B in chickens was performed. Subsequently, virological protection provided from an aMPV/B vaccine against the same isolates was analyzed. Upon challenge experiment, it was shown by virus isolation and real time PCR that aMPV/B could be detected longer and in higher amounts than aMPV/A. For the protection study, 18 one-day-old chicks were vaccinated and challenged at 21 days of age. Using virus isolation and real time PCR, no aMPV/A was detected in the vaccinated chickens, whereas one vaccinated chicken challenged with the aMPV/B isolate was positive. The results showed that aMPV/B vaccine provided a complete heterologous virological protection, although homologous protection was not complete in one chicken. Although only one aMPV/B positive chicken was detected after homologous vaccination, replication in vaccinated animals might allow the emergence of escape mutants.

  18. In vitro antiviral activity of chestnut and quebracho woods extracts against avian reovirus and metapneumovirus.

    Science.gov (United States)

    Lupini, C; Cecchinato, M; Scagliarini, A; Graziani, R; Catelli, E

    2009-12-01

    Field evidences have suggested that a natural extract, containing tannins, could be effective against poultry enteric viral infections. Moreover previous studies have shown that vegetable tannins can have antiviral activity against human viruses. Based on this knowledge three different Chestnut (Castanea spp.) wood extracts and one Quebracho (Schinopsis spp.) wood extract, all containing tannins and currently used in the animal feed industry, were tested for in vitro antiviral activity against avian reovirus (ARV) and avian metapneumovirus (AMPV). The MTT assay was used to evaluate the 50% cytotoxic compounds concentration (CC(50)) on Vero cells. The antiviral properties were tested before and after the adsorption of the viruses to Vero cells. Antiviral activities were expressed as IC(50) (concentration required to inhibit 50% of viral cytopathic effect). CC(50)s of tested compounds were > 200 microg/ml. All compounds had an extracellular antiviral effect against both ARV and AMPV with IC(50) values ranging from 25 to 66 microg/ml. Quebracho extract had also evident intracellular anti-ARV activity (IC(50) 24 microg/ml). These preliminary results suggest that the examined vegetable extracts might be good candidates in the control of some avian virus infections. Nevertheless further in vivo experiments are required to confirm these findings.

  19. Avian metapneumovirus subgroup C induces autophagy through the ATF6 UPR pathway.

    Science.gov (United States)

    Hou, Lei; Wei, Li; Zhu, Shanshan; Wang, Jing; Quan, Rong; Li, Zixuan; Liu, Jue

    2017-10-03

    An increasing number of studies have demonstrated that macroautophagy/autophagy plays an important role in the infectious processes of diverse pathogens. However, it remains unknown whether autophagy is induced in avian metapneumovirus (aMPV)-infected host cells, and, if so, how this occurs. Here, we report that aMPV subgroup C (aMPV/C) induces autophagy in cultured cells. We demonstrated this relationship by detecting classical autophagic features, including the formation of autophagsomes, the presence of GFP-LC3 puncta and the conversation of LC3-I into LC3-II. Also, we used pharmacological regulators and siRNAs targeting ATG7 or LC3 to examine the role of autophagy in aMPV/C replication. The results showed that autophagy is required for efficient replication of aMPV/C. Moreover, infection with aMPV/C promotes autophagosome maturation and induces a complete autophagic process. Finally, the ATF6 pathway, of which one component is the unfolded protein response (UPR), becomes activated in aMPV/C-infected cells. Knockdown of ATF6 inhibited aMPV/C-induced autophagy and viral replication. Collectively, these results not only show that autophagy promotes aMPV/C replication in the cultured cells, but also reveal that the molecular mechanisms underlying aMPV/C-induced autophagy depends on regulation of the ER stress-related UPR pathway.

  20. The pathogenicity of avian metapneumovirus subtype C wild bird isolates in domestic turkeys.

    Science.gov (United States)

    Cha, Ra Mi; Yu, Qingzhong; Zsak, Laszlo

    2013-01-30

    Avian metapneumovirus subtype C (aMPV/C) causes severe upper respiratory disease in turkeys. Previous report revealed the presence of aMPV/C in wild birds in the southeast regions of the U.S. In this study, aMPV/C positive oral swabs from American coots (AC) and Canada geese (CG) were passaged three times in the respiratory tract of specific pathogen free (SPF) turkeys and used as aMPV/C P3 virus isolates in subsequent studies. Wild bird P3 isolates showed similar growth characteristics when compared to virulent aMPV/C in chicken embryo fibroblast ( CEF) cell cultures and their glycoprotein G gene sequence was closely related to the G gene of aMPV/C Colorado reference virus. Three-day-old commercial or SPF turkeys were inoculated oculonasally with wild bird aMPV/C P3 isolates. At 5 and 7 days post-inoculation (DPI), severe clinical signs were observed in both of the AC and CG virus-exposed groups. Viral RNA was detected in tracheal swabs by reverse transcriptase polymerase chain reaction (RT-PCR). In addition, immunohistochemistry showed virus replication in the nasal turbinate and trachea. All virus-exposed turkeys developed positive antibody response by 14 DPI. Our data demonstrate that aMPV/C wild bird isolates induced typical aMPV/C disease in the domestic turkeys.

  1. Investigations on the protective role of passively transferred antibodies against avian metapneumovirus infection in turkeys.

    Science.gov (United States)

    Rubbenstroth, Dennis; Rautenschlein, Silke

    2009-12-01

    The avian metapneumovirus (aMPV) is the causative agent of an acute respiratory disease in turkeys, which causes considerable economic losses to the poultry industry. Currently attenuated live and inactivated vaccines are widely used to control the disease, but vaccine breaks are frequently observed. For improvement of current vaccination strategies it is necessary to gain enhanced knowledge of the immune mechanisms against aMPV infection. Field observations suggest that vaccine-induced aMPV-specific antibodies are not indicative for protection. In the present study we investigated the role of antibodies in protection of turkeys against aMPV. In two experiments, commercial turkey poults received aMPV-specific antibodies by intravenous injection. The antibody transfer resulted in increased antibody levels in the sera. Virus-specific antibodies were also detected on mucosal surfaces such as the trachea, conjunctivae and gall bladder. Turkeys were subsequently challenged with a virulent aMPV subtype A strain. Development of clinical signs, virus detection by polymerase chain reaction and histopathological changes of tracheal mucosa in challenged turkeys with and without passively transferred antibodies were comparable with each other. Our results suggest that humoral immunity does not provide sufficient protection against aMPV infection. Thus, the measurement of vaccine-induced aMPV antibody response may not be considered as an adequate indicator of vaccine efficacy. Further research on the protective role of cell-mediated immune mechanisms is necessary to improve current vaccine strategies.

  2. Molecular detection of infectious bronchitis and avian metapneumoviruses in Oman backyard poultry.

    Science.gov (United States)

    Al-Shekaili, Thunai; Baylis, Matthew; Ganapathy, Kannan

    2015-04-01

    Infectious bronchitis virus (IBV) and avian metapneumovirus (aMPV) are economically important viral pathogens infecting chickens globally. Identification of endemic IBV and aMPV strains promotes better control of both diseases and prevents production losses. Orophrayngeal swab samples were taken from 2317 birds within 243 different backyard flocks in Oman. Swabs from each flock were examined by RT-PCR using part-S1 and G gene primers for IBV and aMPV respectively. Thirty-nine chicken flocks were positive for IBV. Thirty two of these were genotyped and they were closely related to 793/B, M41, D274, IS/1494/06 and IS/885/00. 793/B-like IBV was also found in one turkey and one duck flock. Five flocks were positive for aMPV subtype B. Though no disease was witnessed at the time of sampling, identified viruses including variant IBV strains, may still pose a threat for both backyard and commercial poultry in Oman. Copyright © 2014 Elsevier Ltd. All rights reserved.

  3. Comparative pathogenicity of early and recent isolates of avian metapneumovirus subtype C in turkeys.

    Science.gov (United States)

    Velayudhan, Binu T; Noll, Sally L; Thachil, Anil J; Halvorson, David A; Shaw, Daniel P; Goyal, Sagar M; Nagaraja, Kakambi V

    2008-07-01

    The objective of the present study was to compare the pathogenicity of early and recent isolates of avian metapneumovirus subtype-C (aMPV-C) in turkeys. Two-week-old turkeys were inoculated with early and recent isolates of aMPV-C. Clinical signs were monitored. Tissues were examined for viral ribonucleic acid (RNA), lesions, and viral antigen by reverse transcription-polymerase chain reaction (RT-PCR), histopathology and immunohistochemistry, respectively. Birds infected with the recent isolate had higher clinical sign scores than those infected with the early isolate. Only the recent isolate produced a multifocal loss of cilia in the nasal turbinate of infected birds. Immunohistochemistry revealed intense staining of aMPV antigen in turbinate and trachea of birds infected with the recent isolate. The findings indicate that the recent isolate produced more severe clinical signs and lesions in turkeys compared to the early isolate. The recent isolate could be ideal for the development of a challenge model for aMPV infection in turkeys.

  4. Propagation of avian metapneumovirus subtypes A and B using chicken embryo related and other cell systems.

    Science.gov (United States)

    Coswig, Lia Treptow; dos Santos, Márcia Bianchi; Hafez, Hafez Mohamed; Ferreira, Helena Lage; Arns, Clarice Weis

    2010-07-01

    Primary isolation of avian metapneumovirus (aMPV) is carried out using tracheal organ culture (TOC) or chicken embryonated eggs with subsequent adaptation in chicken embryo fibroblasts (CEF) or Vero cultures. This study was conducted to evaluate six different cell lines and two avian culture systems for the propagation of aMPV subtypes A and B. The chicken embryo related (CER) cells were used successfully for primary isolation. In addition to Vero and baby hamster kidney (BHK-21) cells, CER cells were also shown to be the most appropriate for propagation of aMPV considering high titres. Propagation of A and B subtypes in CEF and TOC remained efficient after the primary isolation and several passages of viruses in the CER cell line. The growth curves were created using CER, Vero and BHK-21 cell lines. Compared with growth, both yielded higher titres in CER cells during the first 30 h after infection, but no significant difference was observed in the results obtained from CER and Vero cells. This data show that CER cells are adequate for aMPV subtypes A and B propagation, giving similar results to Vero cells. Copyright 2010 Elsevier B.V. All rights reserved.

  5. Species-specific deletion of the viral attachment glycoprotein of avian metapneumovirus.

    Science.gov (United States)

    Kong, Byung-Whi; Foster, Linda K; Foster, Douglas N

    2008-03-01

    The avian metapneumovirus (AMPV) genome encodes the fusion (F), small hydrophobic (SH), and attachment glycoprotein (G) as envelope glycoproteins. The F and G proteins mainly function to allow viral entry into host cells during the early steps of the virus life cycle. The highly variable AMPV G protein is a major determinant for distinguishing virus subtypes. Sequence analysis was used to determine if any differences between avian or mammalian cell propagated subtype C AMPV could be detected for the 1.8kb G gene. As a result, the complete 1.8kb G gene was found to be present when AMPV was propagated in our immortal turkey turbinate (TT-1) cell line regardless of passage number. Surprisingly, AMPV propagated for 15 or more passages in mammalian Vero cells revealed an essentially deleted G gene in the viral genome, resulting in no G gene mRNA expression. Although the Vero cell propagated AMPV genome contained a small 122 nucleotide fragment of the G gene, no other mRNA variants were detected from either mammalian or avian propagated AMPV. The G gene truncation might be caused by cellular molecular mechanisms that are species-specific. The lack of viral gene deletions suggests that avian cell propagated AMPV will provide a better alternative host for live recombinant vaccine development based on a reverse genetics system.

  6. Avian metapneumovirus (AMPV) attachment protein involvement in probable virus evolution concurrent with mass live vaccine introduction.

    Science.gov (United States)

    Cecchinato, Mattia; Catelli, Elena; Lupini, Caterina; Ricchizzi, Enrico; Clubbe, Jayne; Battilani, Mara; Naylor, Clive J

    2010-11-20

    Avian metapneumoviruses detected in Northern Italy between 1987 and 2007 were sequenced in their fusion (F) and attachment (G) genes together with the same genes from isolates collected throughout western European prior to 1994. Fusion protein genes sequences were highly conserved while G protein sequences showed much greater heterogeneity. Phylogenetic studies based on both genes clearly showed that later Italian viruses were significantly different to all earlier virus detections, including early detections from Italy. Furthermore a serine residue in the G proteins and lysine residue in the fusion protein were exclusive to Italian viruses, indicating that later viruses probably arose within the country and the notion that these later viruses evolved from earlier Italian progenitors cannot be discounted. Biocomputing analysis applied to F and G proteins of later Italian viruses predicted that only G contained altered T cell epitopes. It appears likely that Italian field viruses evolved in response to selection pressure from vaccine induced immunity. Copyright © 2010 Elsevier B.V. All rights reserved.

  7. B-cell infiltration in the respiratory mucosa of turkeys exposed to subtype C avian metapneumovirus.

    Science.gov (United States)

    Cha, Ra Mi; Khatri, Mahesh; Sharma, Jagdev M

    2007-09-01

    Turkeys exposed to avian metapneumovirus (aMPV) subtype C showed extensive lymphoid cell infiltrations in the nasal turbinates of the upper respiratory tract. The cellular infiltration occurred after the first virus exposure but not after re-exposure. Quantitation of the relative proportions of mucosal immunoglobulin (Ig)A+, IgG+, and IgM+ cells in controls and virus-exposed turkeys revealed that at 7 days after the first virus exposure, when mucosal infiltration was well pronounced, there was a significant increase (P < 0.05) in the numbers of infiltrating IgA+ but not of IgG+ and IgM+ cells. After the second virus exposure, although the overall numbers of mucosal lymphoid cells were similar in the virus-exposed and control turkeys, the relative proportions of IgA+ and IgG+ cells were significantly higher in the virus-exposed turkeys (P < 0.05) than in controls. Furthermore, elevated levels of aMPV-specific IgA were detected in the nasal secretions and the bile of virus-exposed birds after the second but not after the first virus exposure. These results suggest, for the first time, the possible involvement of local mucosal immunoglobulins in the pathogenesis of aMPV in turkeys.

  8. Diagnostic utility of egg yolk for the detection of avian metapneumovirus antibodies in laying hens.

    Science.gov (United States)

    Choi, Kang-Seuk; Lee, Eun-Kyoung; Jeon, Woo-Jin; Park, Mi-Ja; Yoo, Yae-Na; Kwon, Jun-Hun

    2010-12-01

    Surveillance and diagnosis of avian metapneumovirus (AMPV) infection typically involve measurement of serum antibodies. In the current study, eggs instead of serum samples were used for the detection of AMPV antibodies in egg-laying chicken hens by enzyme-linked immunosorbent assay (ELISA). AMPV-free commercial layer hens were experimentally challenged with AMPV strain SC1509 through intravenous or oculonasal administration. Antibody levels were determined by ELISA. AMPV antibodies were detected in egg yolks from challenged hens by 7 days postinoculation (dpi), with the peak titer at 16 dpi. Antibody levels in eggs laid at 28 dpi correlated well (r = 0.93) with sera taken 28 dpi from the same hens. In a field trial of the yolk ELISA, six broiler breeder farms were surveyed, and all tested positive for AMPV antibodies in hen eggs, although positivity varied from farm to farm. Abnormal discolored eggs collected from outbreak farms had significantly higher titers of AMPV yolk antibodies than normal eggs from the same farm, unlike clinically healthy farms, where normal and abnormal eggs had similar antibody titers. These results indicate that diagnosis of AMPV infection by yolk ELISA to detect anti-AMPV antibodies may be a suitable alternative to serologic testing.

  9. Pathogenic and immunogenic responses in turkeys following in ovo exposure to avian metapneumovirus subtype C.

    Science.gov (United States)

    Cha, Ra Mi; Khatri, Mahesh; Mutnal, Manohar; Sharma, Jagdev M

    2011-03-15

    Commercial turkey eggs, free of antibodies to avian metapneumovirus subtype C (aMPV/C), were inoculated with aMPV/C at embryonation day (ED) 24. There was no detectable effect of virus inoculation on the hatchability of eggs. At 4 days post inoculation (DPI) (the day of hatch (ED 28)) and 9 DPI (5 days after hatch), virus replication was detected by quantitative RT-PCR in the turbinate, trachea and lung but not in the thymus or spleen. Mild histological lesions characterized by lymphoid cell infiltration were evident in the turbinate mucosa. Virus exposure inhibited the mitogenic response of splenocytes and thymocytes and upregulated gene expression of IFN-γ and IL-10 in the turbinate tissue. Turkeys hatching from virus-exposed eggs had aMPV/C-specific IgG in the serum and the lachrymal fluid. At 3 week of age, in ovo immunized turkeys were protected against a challenge with pathogenic aMPV/C. Published by Elsevier B.V.

  10. Avian metapneumovirus subtypes circulating in Brazilian vaccinated and nonvaccinated chicken and turkey farms.

    Science.gov (United States)

    Chacón, Jorge Luis; Mizuma, Matheus; Vejarano, Maria P; Toquín, Didier; Eterradossi, Nicolas; Patnayak, Devi P; Goyal, Sagar M; Ferreira, Antonio J Piantino

    2011-03-01

    Avian metapneumovirus (AMPV) causes turkey rhinotracheitis and is associated with swollen head syndrome in chickens, which is usually accompanied by secondary infections that increase mortality. AMPVs circulating in Brazilian vaccinated and nonvaccinated commercial chicken and turkey farms were detected using a universal reverse transcriptase (RT)-PCR assay that can detect the four recognized subtypes of AMPV. The AMPV status of 228 farms with respiratory and reproductive disturbances was investigated. AMPV was detected in broiler, hen, breeder, and turkey farms from six different geographic regions of Brazil. The detected viruses were subtyped using a nested RT-PCR assay and sequence analysis of the G gene. Only subtypes A and B were detected in both vaccinated and nonvaccinated farms. AMPV-A and AMPV-B were detected in 15 and 23 farms, respectively, while both subtypes were simultaneously found in one hen farm. Both vaccine and field viruses were detected in nonvaccinated farms. In five cases, the detected subtype was different than the vaccine subtype. Field subtype B virus was detected mainly during the final years of the survey period. These viruses showed high molecular similarity (more than 96% nucleotide similarity) among themselves and formed a unique phylogenetic group, suggesting that they may have originated from a common strain. These results demonstrate the cocirculation of subtypes A and B in Brazilian commercial farms.

  11. Protection by recombinant viral proteins against a respiratory challenge with virulent avian metapneumovirus.

    Science.gov (United States)

    Chary, Parag; Njenga, M Kariuki; Sharma, Jagdev M

    2005-12-15

    Protection by recombinant avian metapneumovirus (aMPV) N or M proteins against a respiratory challenge with virulent aMPV was examined. N, M or N+M proteins were administered intramuscularly (IM) with incomplete Freund's adjuvant (IFA) or by the oculonasal (ON) route with cholera toxin-B (CTB). Each turkey received 40 or 80 microg of each recombinant protein. Birds were considered protected against challenge if the challenge virus was not detectable in the choanal swabs by RT-PCR. At a dose of 40 microg/bird, N protein given with IFA by the IM route protected eight out of nine birds. M protein at the same dose protected three out of seven birds, while a combination of N+M proteins (40 microg each) protected three out of four birds. At a dose of 80 microg of each of N and M proteins per bird given with IFA by the IM route, 100% protection was achieved. ON immunization with a mixture of N and M proteins induced partial protection when the proteins were given with CTB; no detectable protection was noted without CTB. N and M proteins induced anti-aMPV antibodies, although protection against virulent virus challenge did not appear to be associated with the level or presence of antibodies.

  12. Protection against avian metapneumovirus subtype C in turkeys immunized via the respiratory tract with inactivated virus.

    Science.gov (United States)

    Cha, Ra Mi; Khatri, Mahesh; Sharma, Jagdev M

    2011-01-10

    Avian metapneumovirus subtype C (aMPV/C) causes a severe upper respiratory tract (URT) infection in turkeys. Turkeys were inoculated oculonasally with inactivated aMPV/C adjuvanted with synthetic double-stranded RNA polyriboinosinic polyribocytidylic acid (Poly IC). Immunized turkeys had elevated numbers of mucosal IgA+ cells in the URT and increased levels of virus-specific IgG and IgA in the lachrymal fluid and IgG in the serum. After 7 or 21 days post immunization, turkeys were challenged oculonasally with pathogenic aMPV/C. Immunized groups were protected against respiratory lesions induced by the challenge virus. Further, the viral copy number of the challenge virus in the URT were significantly lower in the immunized turkeys than in the unimmunized turkeys (P<0.05). These results showed that inactivated aMPV/C administered by the respiratory route induced protective immunity against pathogenic virus challenge. Copyright © 2010 Elsevier Ltd. All rights reserved.

  13. Oxitocina e comportamento humano Oxitocina y comportamiento humano Oxytocin and human behavior

    OpenAIRE

    Diana Catarina Ferreira de Campos; João Manuel Garcia do Nascimento Graveto

    2010-01-01

    Recentemente, têm surgido numerosas especulações sobre o papel da oxitocina nas emoções e relações sociais do ser humano. O presente artigo retrata um estudo essencialmente reflexivo sobre o papel da oxitocina no comportamento humano. A pesquisa bibliográfica foi efectuada nas bases de dados MEDLINE, versão PubMed e CINAHL. Os principais resultados das pesquisas sustentam que os níveis de oxitocina aumentam em resposta à aplicação de massagem e indução de emoções positivas. A administração in...

  14. Population Dynamics and Rates of Molecular Evolution of a Recently Emerged Paramyxovirus, Avian Metapneumovirus Subtype C▿ †

    OpenAIRE

    Padhi, Abinash; Poss, Mary

    2008-01-01

    We report the existence of two distinct sublineages of avian metapneumovirus (MPV) subtype C, a virus which has caused serious economic loss in commercial turkey farms in the United States. This subtype is closely related to human MPV, infects multiple avian species, and is globally distributed. The evolutionary rates of this virus are estimated to be 1.3 × 10−3 to 7 × 10−3 substitutions per site per year, and coalescent estimates place its emergence between 1991 and 1996. The four genes exam...

  15. Population Dynamics and Rates of Molecular Evolution of a Recently Emerged Paramyxovirus, Avian Metapneumovirus Subtype C▿ †

    Science.gov (United States)

    Padhi, Abinash; Poss, Mary

    2009-01-01

    We report the existence of two distinct sublineages of avian metapneumovirus (MPV) subtype C, a virus which has caused serious economic loss in commercial turkey farms in the United States. This subtype is closely related to human MPV, infects multiple avian species, and is globally distributed. The evolutionary rates of this virus are estimated to be 1.3 × 10−3 to 7 × 10−3 substitutions per site per year, and coalescent estimates place its emergence between 1991 and 1996. The four genes examined show a concordant demographic pattern which is characterized by a rapid increase in population size followed by stable population grown until the present. PMID:19052092

  16. ¿Qué son los derechos humanos?

    Directory of Open Access Journals (Sweden)

    Daniel Raffo

    2015-01-01

    Full Text Available Sobre los Derechos humanos se habló pasada la Segunda Guerra Mundial. El autor da varias definiciones y se enfatiza en que son derechos inherentes a la persona humana, derechos naturales del hombre, derechos fundamentales, son parte de la misma cosa: Defender al ser humano del ser humano.Precisa tres corrientes: la absolutista, la subjetivista y la relativa. Educar y concienciar es la tarea y en esto los medios de comunicación tienen su labor.

  17. Effects of Cyclosporin A induced T-lymphocyte depletion on the course of avian Metapneumovirus (aMPV) infection in turkeys

    DEFF Research Database (Denmark)

    Rubbenstroth, Dennis; Dalgaard, Tina S; Kothlow, Sonja

    2010-01-01

    The avian Metapneumovirus (aMPV) causes an economically important acute respiratory disease in turkeys (turkey rhinotracheitis, TRT).While antibodies were shownto be insufficient for protection against a MPV-infection, the role of T-lymphocytes in the control of aMPV-infection is not clear...

  18. Biological assessment of recombinant avian metapneumovirus subgroup C (aMPV-C) viruses containing different length of the G gene in cultured cells and SPF turkeys.

    Science.gov (United States)

    Genetic variation in length of the glycoprotein (G) gene among different avian metapneumovirus subgroup C (aMPV-C) isolates has been reported. However, its biological significance in virus replication and pathogenicity is unknown. In this study, we generated two Colorado (CO) strain-based recombinan...

  19. Generation of recombinant newcastle disease viruses, expressing the glycoprotein (G) of avian metapneumovirus, subtype A, or B, for use as bivalent vaccines

    Science.gov (United States)

    Using reverse genetics technology, Newcastle disease virus (NDV) LaSota strain-based recombinant viruses were engineered to express the glycoprotein (G) of avian metapneumovirus (aMPV), subtype A, or B, as bivalent vaccines. These recombinant viruses, rLS/aMPV-A G and rLS/aMPV-B G, were slightly att...

  20. Deletion of the M2-2 Gene from Avian Metapneumovirus Subgroup C (aMPV-C) Impairs Virus Replication and Immunogenicity in Turkeys

    Science.gov (United States)

    The second matrix (M2) gene of avian metapneumovirus subgroup C (aMPV-C) virus contains two overlapping open reading frames (ORFs), encoding two putative proteins, M2-1 and M2-2. Both proteins are believed to be involved in either viral RNA transcription or replication. To further characterize the f...

  1. DERECHOS HUMANOS Y VIVIR BIEN / BUEN VIVIR

    Directory of Open Access Journals (Sweden)

    Adalid Contreras Baspineiro

    2017-03-01

    Full Text Available La cosmovisión del Vivir Bien / Buen Vivir es, en su naturaleza y en su constitución, una expresión anticipatoria de la formulación sistematizada de los Derechos Humanos integrales; en realidad construir la sociedad del Vivir Bien/Buen Vivir es construir una sociedad de plena vigencia de los derechos humanos y de los derechos de la naturaleza. El presente artículo tiene el propósito de mostrar esta interdependencia a partir del análisis crítico sobre su realización en dos campos políticos[1]: el comunitario y el estatal. El campo político comunitario teje articulaciones entre una cosmovisión originada en los pueblos indígenas, las reivindicaciones de grupos ciudadanos por la exigibilidad de los derechos humanos, y la doctrina social de la Iglesia que vela por el bien común. El campo político estatal se expresa en el impulso de un nuevo constitucionalismo basado en los derechos y en políticas nacionales de desarrollo para el Vivir Bien / Buen Vivir. [1] Siguiendo a Bourdieu, reconocemos que el campo político articula un nivel de acción ideológica y estratégica relacionada con la hegemonía del poder, otro institucional en el que se identifican los actores políticos, y un tercero en el que se definen los principios y normas.

  2. O DIREITO HUMANO AO ACESSO À INTERNET

    Directory of Open Access Journals (Sweden)

    Manuel Martín Pino Estrada

    2015-12-01

    Full Text Available O acesso à internet tornou-se essencial na vida das pessoas. Estas podem manifestar as suas ideias, fazer denúncias, colocar vídeos, postar imagens, procurar emprego, fazer amizades, convocar protestos, incentivar grupos para atividades diversas, mobilizar gente em prol de reivindicação de direitos, assim como para conquistá-los, pedir ajuda, estudar e adquirir conhecimento, dentre outras atividades afins. Então, ao tornar-se tão importante, tornou-se também um direito humano, que não pode ser mais retirado da rede.

  3. Pobreza, capital humano, capital social e familiar

    OpenAIRE

    Petrini, Giancarlo; Fonseca, Ricardo; Porreca, Wladimir

    2010-01-01

    O presente estudo investiga a pobreza partindo de uma análise dos recursos que os pobres dispõem em sua realidade, analisando os temas do capital humano, social e familiar, procurando estabelecer conexões e elucidar fatores da realidade pouco considerados em estudos e  projetos de combate à pobreza e à exclusão social. O presente artigo, em seu conjunto, procura compreender porque, em condições semelhantes de pobreza, algumas pessoas conseguem elaborar um projeto de vida enquanto outras se es...

  4. Prostitución y derechos humanos

    Directory of Open Access Journals (Sweden)

    Daniela Heim

    2011-06-01

    Full Text Available RESUMEN Este artículo analiza las principales contribuciones de los movimientos feministas de cara a la comprensión del complejo fenómeno de la prostitución y sus diferentes estrategias en orden a la vindicación, reconocimiento y protección de los derechos humanos de las mujeres insertadas en esta actividad.   ABSTRACT This article analyzes the main contributions of the feminist movements in order to understand the complex phenomenon of the prostitution and its different strategies of vindication, recognition and protection of the human rights of women inserted in this activity.

  5. Servicios públicos y derechos humanos

    OpenAIRE

    Cabello, Julia

    2004-01-01

    Cuando se habla o se escribe sobre los Derechos Humanos en el Perú, casi siempre el imaginario colectivo se traslada a las imágenes de torturas, asesinatos y desapariciones de pobladores y dirigentes sindicales y populares que se dieron en los diferentes gobiernos de turno; y que se recrudeciera en la década de los 90 en el nefasto régimen del Gobierno corrupto de Alberto Fujimori. Sin embargo, en el Perú como en toda América Latina, no solo los derechos civiles y políticos son vulnerado...

  6. La anatomia del cuerpo humano: exposicion temporal

    OpenAIRE

    Antommarchi, Francesco

    2013-01-01

    El cuerpo humano representado en la obra de Paolo Mascagni y posteriormente en la de Antommarchi, plasma en detalle la totalidad de los órganos del cuerpo como el anatomista los podía ver en un cadáver: desde la capa superficial subyacente a los tegumentos comunes, hasta el esqueleto; la red subcutánea formada por los nervios y los vasos sanguíneos superficiales, todos los planos musculares con los nervios, los vasos sanguíneos y los vasos linfáticos; en fin, los órganos que componen las tres...

  7. Avian metapneumovirus subtype B experimental infection and tissue distribution in chickens, sparrows, and pigeons.

    Science.gov (United States)

    Gharaibeh, S; Shamoun, M

    2012-07-01

    Avian metapneumovirus (aMPV) is a respiratory virus that infects a range of avian hosts, including chickens and turkeys. Migratory and local wild birds are implicated in aMPV spread among farms, countries, and seasonal outbreaks of the disease. A subtype B aMPV isolate from commercial chicken flocks suffering from respiratory disease was experimentally inoculated oculonasally into 7-week old chickens, young pigeons, and sparrows. Chickens showed minimal tracheal rales, whereas pigeons and sparrows were asymptomatic. Shedding of aMPV was detected by reverse transcription polymerase chain reaction on homogenates from nasal turbinates. At 5 days postinfection, 5 of 5 chickens, 2 of 5 pigeons, and 1 of 5 sparrows were positive; at 10 or 15 days, none were positive. At 2 and 5 days, aMPV antigens were localized at the ciliated boarder of respiratory epithelium in nasal cavity and trachea of chickens, as well as to the conjunctival epithelium. Pigeons had detectable viral antigens in only the trachea at 2 and 5 days; sparrow tissues did not show any positive staining. At the end of the experiment, at 21 days postinfection, 14 of 15 inoculated chickens seroconverted against aMPV, but none of the inoculated pigeons or sparrows did. The authors believe that pigeons and sparrows have the ability to transmit the virus between chicken farms, although they do not consider pigeons and sparrows as natural hosts for aMPV, given that they failed to seroconvert. In conclusion, pigeons and sparrows are partially susceptible to aMPV infection, probably acting more as mechanical vectors because infection is only temporary and short-lived.

  8. First evidence of avian metapneumovirus subtype A infection in turkeys in Egypt.

    Science.gov (United States)

    Abdel-Azeem, Abdel-Azeem Sayed; Franzo, Giovanni; Dalle Zotte, Antonella; Drigo, Michele; Catelli, Elena; Lupini, Caterina; Martini, Marco; Cecchinato, Mattia

    2014-08-01

    Although avian metapneumovirus (aMPV) infection has been reported in most regions of the world, to date, only subtype B has been detected in Egypt. At the end of November 2013, dry oropharyngeal swabs were collected during an outbreak of respiratory diseases in a free-range, multi-age turkey dealer farm in Northern Upper Egypt. The clinical signs that appeared when turkeys were 3 weeks-old were characterized by ocular and nasal discharge and swelling of sinuses. aMPV of subtype A was detected by real-time reverse transcription-polymerase chain reaction. In order to confirm the results and obtain more information on the molecular characteristics of the virus, F and G protein genes were partially sequenced and compared with previously published sequences deposited in GenBank by using BLAST. Subtype of the strain was confirmed by sequencing of partial F and G protein genes. The highest percentages of identity were observed when G sequence of the Egyptian strain was compared with the sequence of an aMPV-A isolated in Nigeria (96.4 %) and when the F sequence was compared with strains isolated respectively in Italy and in UK (97.1 %). Moreover, the alignment of the sequences with commercial subtype A vaccine or vaccine-derived strains showed differences in the Egyptian strain that indicate its probable field origin. The detection of aMPV in the investigated turkey flock highlights some relevant epidemiological issues regarding the role that multi-age farms and dealers may play in perpetuating aMPV infection within and among farms. To our knowledge, this is the first report of aMPV subtype A in Egypt.

  9. Analysis of expression and glycosylation of avian metapneumovirus attachment glycoprotein from recombinant baculoviruses.

    Science.gov (United States)

    Luo, Lizhong; Nishi, Krista; MacLeod, Erin; Sabara, Marta I; Li, Yan

    2010-11-01

    Recently, we reported the expression and glycosylation of avian metapneumovirus attachment glycoprotein (AMPV/C G protein) in eukaryotic cell lines by a transient-expression method. In the present study, we investigated the biosynthesis and O-linked glycosylation of the AMPV/C G protein in a baculovirus expression system. The results showed that the insect cell-produced G protein migrated more rapidly in SDS-PAGE as compared to LLC-MK2 cell-derived G proteins owing to glycosylation differences. The fully processed, mature form of G protein migrated between 78 and 86 kDa, which is smaller than the 110 kDa mature form of G expressed in LLC-MK2 cells. In addition, several immature G gene products migrating at 40-48 and 60-70 kDa were also detected by SDS-PAGE and represented glycosylated intermediates. The addition of the antibiotic tunicamycin, which blocks early steps of glycosylation, to insect cell culture resulted in the disappearance of two glycosylated forms of the G protein and identified a 38 kDa unglycosylated precursor. The maturation of the G protein was completely blocked by monensin, suggesting that the O-linked glycosylation of G initiated in the trans-Golgi compartment. The presence of O-linked sugars on the mature protein was further confirmed by lectin Arachis hypogaea binding assay. Furthermore, antigenic features of the G protein expressed in insect cells were evaluated by ELISA. Crown Copyright © 2010. Published by Elsevier B.V. All rights reserved.

  10. Emergence of a virulent type C avian metapneumovirus in turkeys in Minnesota.

    Science.gov (United States)

    Velayudhan, Binu T; McComb, Brian; Bennett, Richard S; Lopes, Vanessa C; Shaw, Daniel; Halvorson, David A; Nagaraja, Kakambi V

    2005-12-01

    The objectives of the present study were to investigate the pathogenesis of a recent isolate of avian metapneumovirus (aMPV) in turkeys and to evaluate the quantitative distribution of the virus in various tissues during the course of infection. Seventy 2-week-old turkey poults were divided equally into two groups. One group was inoculated with aMPV (MN 19) with a titer of 10(5.5) TCID50 oculonasally. Birds in the second group were maintained as sham-inoculated controls. Birds showed severe clinical signs in the form of copious nasal discharge, swollen sinus, conjunctivitis, and depression from 4 days postinoculation (PI) to 12 days PI. Samples from nasal turbinates, trachea, conjunctiva, Harderian gland, infraorbital sinus, lungs, liver, and spleen were collected at 1, 3, 5, 7, 9, 11, and 14 days PI. Histopathologic lesions such as a multifocal loss of cilia were prominent in nasal turbinate and were seen from 3 to 11 days PI. Immunohistochemistry revealed the presence of aMPV from 3 to 9 days PI in nasal turbinate and trachea. Viral RNA could be detected for 14 days PI from nasal turbinate and for 9 days from trachea. In situ hybridization demonstrated the presence of aMPV from 1 to 11 days PI in nasal turbinates and from 3 to 9 days PI in the trachea. Quantitative real-time polymerase chain reaction data showed the presence of a maximum amount of virus at 3 days PI in nasal turbinate and trachea. Clinically and histopathologically, the new isolate appears to be more virulent compared to the early isolates of aMPV in the United States.

  11. Glycoprotein gene truncation in avian metapneumovirus subtype C isolates from the United States.

    Science.gov (United States)

    Velayudhan, Binu T; Yu, Qingzhong; Estevez, Carlos N; Nagaraja, Kakambi V; Halvorson, David A

    2008-10-01

    The length of the published glycoprotein (G) gene sequences of avian metapneumovirus subtype-C (aMPV-C) isolated from domestic turkeys and wild birds in the United States (1996-2003) remains controversial. To explore the G gene size variation in aMPV-C by the year of isolation and cell culture passage levels, we examined 21 turkey isolates of aMPV-C at different cell culture passages. The early domestic turkey isolates of aMPV-C (aMPV/CO/1996, aMPV/MN/1a-b, and 2a-b/97) had a G gene of 1,798 nucleotides (nt) that coded for a predicted protein of 585 amino acids (aa) and showed >97% nt similarity with that of aMPV-C isolated from Canada geese. This large G gene got truncated upon serial passages in Vero cell cultures by deletion of 1,015 nt near the end of the open reading frame. The recent domestic turkey isolates of aMPV-C lacked the large G gene but instead had a small G gene of 783 nt, irrespective of cell culture passage levels. In some cultures, both large and small genes were detected, indicating the existence of a mixed population of the virus. Apparently, serial passage of aMPV-C in cell cultures and natural passage in turkeys in the field led to truncation of the G gene, which may be a mechanism of virus evolution for survival in a new host or environment.

  12. Avian metapneumovirus infection of chicken and turkey tracheal organ cultures: comparison of virus-host interactions.

    Science.gov (United States)

    Hartmann, Sandra; Sid, Hicham; Rautenschlein, Silke

    2015-01-01

    Avian metapneumovirus (aMPV) is a pathogen with worldwide distribution, which can cause high economic losses in infected poultry. aMPV mainly causes infection of the upper respiratory tract in both chickens and turkeys, although turkeys seem to be more susceptible. Little is known about virus-host interactions at epithelial surfaces after aMPV infection. Tracheal organ cultures (TOC) are a suitable model to investigate virus-host interaction in the respiratory epithelium. Therefore, we investigated virus replication rates and lesion development in chicken and turkey TOC after infection with a virulent aMPV subtype A strain. Aspects of the innate immune response, such as interferon-α and inducible nitric oxide synthase mRNA expression, as well as virus-induced apoptosis were determined. The aMPV-replication rate was higher in turkey (TTOC) compared to chicken TOC (CTOC) (P < 0.05), providing circumstantial evidence that indeed turkeys may be more susceptible. The interferon-α response was down-regulated from 2 to 144 hours post infection in both species compared to virus-free controls (P < 0.05); this was more significant for CTOC than TTOC. Inducible nitric oxide synthase expression was significantly up-regulated in aMPV-A-infected TTOC and CTOC compared to virus-free controls (P < 0.05). However, the results suggest that NO may play a different role in aMPV pathogenesis between turkeys and chickens as indicated by differences in apoptosis rate and lesion development between species. Overall, our study reveals differences in innate immune response regulation and therefore may explain differences in aMPV - A replication rates between infected TTOC and CTOC, which subsequently lead to more severe clinical signs and a higher rate of secondary infections in turkeys.

  13. Orchitis in roosters with reduced fertility associated with avian infectious bronchitis virus and avian metapneumovirus infections.

    Science.gov (United States)

    Villarreal, L Y B; Brandão, P E; Chacón, J L; Assayag, M S; Maiorka, P C; Raffi, P; Saidenberg, A B S; Jones, R C; Ferreira, A J P

    2007-12-01

    The pathogenesis of infection involving both infectious bronchitis virus (IBV) and avian metapneumovirus (aMPV) causes reproductive damage in hens after viral replication in the epithelium of the oviduct, resulting in loss of cilia and degeneration and necrosis of the epithelial and glandular cells. Although IBV has been indicated as a possible cause of the formation of calcium stones in the epididymus of roosters, a definitive association has not been confirmed. This report describes the detection of IBV and aMPV in the testes of roosters from a Brazilian poultry broiler breeder's flock with epididymal stones and low fertility. Samples of testis, trachea, and lungs from breeder males aged 57 wk were positive for IBV by reverse transcriptase-polymerase chain reaction (RT-PCR), and virus isolation and testis samples were also positive for aMPV by RT-PCR. The inoculation of testis samples into embryonated chicken eggs via the allantoic cavity resulted in curled, hemorrhagic, and stunted embryos typical of IBV infection. The allantoic fluid was positive by RT-PCR aimed to amplify the region coding for the S1 subunit of the IBV S gene, but it was not positive for aMPV. Sequence analysis of the amplified fragment revealed a close relationship with European IBV genotype D274, previously unreported in Brazil. These results indicate that IBV and perhaps aMPV are likely to have played a role in the pathogenesis of the testicular disease described and should be regarded as factors that can influence male fertility disease in chickens.

  14. Stability of the glycoprotein gene of avian metapneumovirus (Canada goose isolate 15a/01) after serial passages in cell cultures.

    Science.gov (United States)

    Chockalingam, Ashok K; Chander, Yogesh; Halvorson, David A; Goyal, Sagar M

    2010-06-01

    The glycoprotein (G) gene sequences of avian metapneumovirus (aMPV) subtypes A, B, C, and D are variable in size and number of nucleotides. The G gene of early U.S. turkey isolates of aMPV-C have been reported to be 1798 nucleotides (nt) (585 aa) in length, whereas the G genes of more recent turkey isolates have been reported to be 783 nucleotides. In some studies, the G gene of aMPV-C turkey isolates was found to be truncated to a smaller G gene of 783 nt (261 aa) upon serial passages in Vero cells. This is believed to be due to the deletion of 1015 nt near the end of the open reading frame. The purpose of this study was to determine variation, if any, in the G gene of an aMPV-C isolated from a wild bird (Canada goose [Branta canadensis]) following serial passages in Vero cells. No size variation was observed for up to 50 passages, except for a few amino acid changes in the extracellular domain at the 50th passage level. The G gene of this wild bird isolate appears to be unique from subtype C metapneumoviruses of turkeys.

  15. CAPITAL HUMANO Y E-RRHH

    Directory of Open Access Journals (Sweden)

    Carlos Ramón López Paz

    2007-05-01

    Full Text Available

    Se expresan consideraciones acerca de la necesidad de la integración de concepciones y técnicas sobre el tratamiento del capital humano y el capital intelectual, con concepciones y técnicas relativas a las nuevas tecnologías de la información y las comunicaciones (NTIC, para el logro de e-RRHH, destacando entre estas últimas los

    data warehouses y data mining. Al gestor de recursos humanos de esta contemporaneidad corresponde desarrollar las acciones pertinentes, o tendrá que renunciar a tal gestión.

  16. Charles Darwin: um observador do desenvolvimento humano

    Directory of Open Access Journals (Sweden)

    Eloisa Helena Rubello Valler Celeri

    2010-12-01

    Full Text Available Os autores traduzem, pela primeira vez para o português, o artigo de Charles Darwin "A Biographical Sketch of an Infant", publicado no periódico Mind em julho de 1877. Utilizando anotações de observações do desenvolvimento de seus filhos, especialmente de seu filho mais velho William Erasmus (Doddy, Darwin descreve e estuda, a partir de seu enfoque naturalista, o filhote humano, narrando os primeiros indicativos comportamentais de emoções tais como raiva e medo, curiosidade e senso moral, o brincar e o prazer envolvido nesta atividade, a capacidade de imitação e os primeiros indícios daquilo que hoje conhecemos como "teoria da mente". Colocando-se questões sobre as capacidades do bebê, como eles aprendem e como se comunicam e levantando hipóteses sobre possíveis significados de certos comportamentos, questões ainda hoje fundamentais para o estudo do desenvolvimento humano, Darwin mostra-se também um pioneiro no estudo do bebê e da criança pequena, numa época na qual as capacidades dos bebês eram extremamente subestimadas e desconsideradas.

  17. Cárceles y derechos humanos en México

    OpenAIRE

    Comisión Nacional de los Derechos Humanos

    2004-01-01

    Síntesis del informe especial de la Comisión Nacional de los Derechos Humanos sobre la situación de los derechos humanos en los centros de reclusión de la república mexicana, dependientes de gobiernos locales y municipales.

  18. DESARROLLO HUMANO: A TRAVES DE LOS OBJETIVOS FUNDAMENTALES TRANSVERSALES (OFT)

    OpenAIRE

    Mulsow G.,Gloria

    2000-01-01

    El desarrollo humano es tarea de todos y en especial del proceso de educación. Los objetivos fundamentales transversales (OFT) son un medio para desarrollar algunas actividades tendientes a favorecer el desarrollo humano de las personas, en este caso de los educandos. Operacionalizar los objetivos fundamentales en sus diferentes ámbitos es el objetivo del presente trabajo.

  19. About human genome Acerca del genoma humano

    Directory of Open Access Journals (Sweden)

    Mojica Tobias

    2000-12-01

    Full Text Available The sequence ofthe human genome, an undertaking ofadvanced countries, is nearly complete. In fact The Human Genome Project has around 85% ofthe genome sequenced 4 times on the average, with an accuracy of roughly 1 in 1000 nucleotides. Celera Genomics, on the other hand, has 99% of the sequence of one person, with an accuracy of slightly less than 1 in 100. The Human Genome project trives to produce a physical map for public consumption following a step by step strategy, in which the researcher sequences short DNA fragments belonging to Iarger fragments of known relative
    position. Celera Genomics wants to have very rapidly a physical map which can be quickly used to develop genetic tests and drugs, which can be later sold. We feel that the sequence ofthe human genome is something, which will widen the gap between advanced and backward countries.En este artículo se revisan los eventos, alrededor del secuenciamiento del genoma humano, que han llevado a tanta excitación en los medios noticiosos y académicos en meses recientes. Se explican las estrategias que han llevado a que tengamos dos borradores diferentes pero complementarios, la estrategia llevada a cabo con el dinero
    de los contribuyentes que consiste en establecer el orden de fragmentos grandes de DNA antes de ser secuenciados y la estrategia llevada a cabo con dineros aportados por la industria privada, con la intención de explotar gananciosamente el conocimiento derivado del genoma humano. El genoma humano a mediados del año 2000 es
    un borrador incompleto que cubre aliededor del 85% de la secuencia con una precisión de un error en 1000 y el 99% de la secuencia con una precisión menor de 1 en 100 nucleótidos, También se discuten algunas de las posibles avenidas

  20. Direitos humanos e OEA. Processos e procedimentos

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    Christian Perrone

    2015-08-01

    Full Text Available Este artigo busca preencher a lacuna no tratamento do Direito Internacional dos Direitos Humanos, focando nos procedimentos internos dos organismos internacionais que acabam por dar corpo (positiva ou negativamente para estes conceitos jurídicos. Os três principais eixos serão os órgãos da Organização dos Estados Americanos que tratam destes direitos, os procedimentos gerais, e os mecanismos individuais de proteção. Como conclusão, tem-se que toda a Organização é responsável pela realização, desenvolvimento e proteção de direitos e que a sociedade coletiva ou individualmente possui espaço de influência através de mecanismos próprios.

  1. Complejidad del dolor y el sufrimiento humano

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    Carlos Rojas Malpica

    Full Text Available En el presente trabajo se discute una cierta concepción unidireccional que, desde el positivismo lógico, se ha venido imponiendo en la investigación médico-psiquiátrica. Aunque la mayoría de los enfermos y de los investigadores insistan en presentar y/o estudiar el dolor y el sufrimiento humano a partir de sus características más molestas y desagradables, estos son fenómenos complejos que no admiten esa sola lectura. Un recorrido por la filosofía, la fenomenología y el psicoanálisis nos aproximará a posibilidades poco exploradas por la medicina, para comprender mejor la complejidad del problema más allá de los síntomas y de la neurofisiología.

  2. Direitos Humanos em Kant e Habermas

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    Alessandro Pinzani

    2011-12-01

    Full Text Available http://dx.doi.org/10.5007/1677-2954.2011v10n1p179 O livro de Milene Tonetto é mais ambicioso do que o título poderia deixar pensar. Longe de limitar-se a uma exposição da temática dos direitos humanos nos dois autores em questão, o texto representa uma contribuição importante à discussão acerca dos fundamentos do direito em Kant e da relação entre direito e moral na obra deste pensador, assim como à discussão acerca da fundamentação do direito oferecida por Habermas.

  3. El sentido humano entre enfermera y paciente

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    Erika Nathalia Rojas-Moreno

    2006-12-01

    Full Text Available Este ensayo retoma la Enfermería como una profesión de gran importancia a nivel mundial, no solo por la preparación técnica, científica e investigativa que la caracteriza, sino por aquel sentido humano que la identifica y a la vez la diferencia de todas las demos carreras formativas en el área de la salud. Sin embargo, en la actualidad surge una enorme preocupación debido a que en el ejercicio de la labor, los enfermeros (as se han olvidado que los pacientes, más que instrumentos de trabajo son seres humanos que necesitan de un amigo cercano para superar la enfermedad que afecta su cuerpo y a la vez entristece su espíritu. Por esta razón se retoma la teoría de la Enfermería Psicodinornica de Hildegard E. Peplau a fin de recordar los seis roles frol de extrana, rol de suministradora de recursos, rol de educadora, rol de !icier, rol de sustituta y rol de consejera y las cuatro fases (Orientación, identificación, exploración y resolución], que de manera sencilla y concreta, enmarcan nuestra significativa labor en la relación enfermera-paciente. El ensayo finaliza presentando un breve análisis de lo que una relación de ayuda puede hacer en beneficio de la vida del paciente y del profesional de enfermería puesto que a lo largo del tiempo se ha demostrado que esta, contribuye en gran medida al crecimiento personal del individuo.

  4. TMPRSS12 Is an Activating Protease for Subtype B Avian Metapneumovirus.

    Science.gov (United States)

    Yun, Bingling; Zhang, Yao; Liu, Yongzhen; Guan, Xiaolu; Wang, Yongqiang; Qi, Xiaole; Cui, Hongyu; Liu, Changjun; Zhang, Yanping; Gao, Honglei; Gao, Li; Li, Kai; Gao, Yulong; Wang, Xiaomei

    2016-12-15

    The entry of avian metapneumovirus (aMPV) into host cells initially requires the fusion of viral and cell membranes, which is exclusively mediated by fusion (F) protein. Proteolysis of aMPV F protein by endogenous proteases of host cells allows F protein to induce membrane fusion; however, these proteases have not been identified. Here, we provide the first evidence that the transmembrane serine protease TMPRSS12 facilitates the cleavage of subtype B aMPV (aMPV/B) F protein. We found that overexpression of TMPRSS12 enhanced aMPV/B F protein cleavage, F protein fusogenicity, and viral replication. Subsequently, knockdown of TMPRSS12 with specific small interfering RNAs (siRNAs) reduced aMPV/B F protein cleavage, F protein fusogenicity, and viral replication. We also found a cleavage motif in the aMPV/B F protein (amino acids 100 and 101) that was recognized by TMPRSS12. The histidine, aspartic acid, and serine residue (HDS) triad of TMPRSS12 was shown to be essential for the proteolysis of aMPV/B F protein via mutation analysis. Notably, we observed TMPRSS12 mRNA expression in target organs of aMPV/B in chickens. Overall, our results indicate that TMPRSS12 is crucial for aMPV/B F protein proteolysis and aMPV/B infectivity and that TMPRSS12 may serve as a target for novel therapeutics and prophylactics for aMPV. Proteolysis of the aMPV F protein is a prerequisite for F protein-mediated membrane fusion of virus and cell and for aMPV infection; however, the proteases used in vitro and vivo are not clear. A combination of analyses, including overexpression, knockdown, and mutation methods, demonstrated that the transmembrane serine protease TMPRSS12 facilitated cleavage of subtype B aMPV (aMPV/B) F protein. Importantly, we located the motif in the aMPV/B F protein recognized by TMPRSS12 and the catalytic triad in TMPRSS12 that facilitated proteolysis of the aMPV/B F protein. This is the first report on TMPRSS12 as a protease for proteolysis of viral envelope

  5. Os Direitos Humanos e o Direito Internacional do Meio Ambiente

    OpenAIRE

    Vitória Schmidt, Rafael

    2010-01-01

    Este artigo objetiva analisar, no que se refere ao direito internacional, em que medida a proteção ao meio ambiente se insere na relação dos direitos humanos fundamentais. Até o marco estabelecido pela declaração de Estocolmo de 1972, o meio ambiente era considerado, no plano global, uma questão alienada da humanidade e dos direitos humanos. Atualmente, o já denominado Direito Internacional do Meio Ambiente, ao lado da proteção internacional dos Direitos Humanos, constitui um dos temas princi...

  6. Viral replication and lung lesions in BALB/c mice experimentally inoculated with avian metapneumovirus subgroup C isolated from chickens.

    Directory of Open Access Journals (Sweden)

    Li Wei

    Full Text Available Avian metapneumovirus (aMPV emerged as an important respiratory pathogen causing acute respiratory tract infection in avian species. Here we used a chicken aMPV subgroup C (aMPV/C isolate to inoculate experimentally BALB/c mice and found that the aMPV/C can efficiently replicate and persist in the lungs of mice for at least 21 days with a peak viral load at day 6 postinoculation. Lung pathological changes were characterized by increased inflammatory cells. Immunochemical assay showed the presence of viral antigens in the lungs and significant upregulation of pulmonary inflammatory cytokines and chemokines including MCP-1, MIP-1α, RANTES, IL-1β, IFN-γ, and TNF-α were detected following inoculation. These results indicate for the first time that chicken aMPV/C may replicate in the lung of mice. Whether aMPV/C has potential as zoonotic pathogen, further investigation will be required.

  7. Mucosal vaccination with formalin-inactivated avian metapneumovirus subtype C does not protect turkeys following intranasal challenge.

    Science.gov (United States)

    Kapczynski, Darrell R; Perkins, Laura L; Sellers, Holly S

    2008-03-01

    Studies were performed to determine if mucosal vaccination with inactivated avian metapneumovirus (aMPV) subtype C protected turkey poults from clinical disease and virus replication following mucosal challenge. Decreases in clinical disease were not observed in vaccinated groups, and the vaccine failed to inhibit virus replication in the tracheas of 96% of vaccinated birds. Histopathologically, enhancement of pulmonary lesions following virus challenge was associated with birds receiving the inactivated aMPV vaccine compared to unvaccinated birds. As determined by an enzyme-linked immunosorbent assay (ELISA), all virus-challenged groups increased serum immunoglobulin (Ig) G and IgA antibody production against the virus following challenge; however, the unvaccinated aMPV-challenged group displayed the highest increases in virus-neutralizing antibody. On the basis of these results it is concluded that intranasal vaccination with inactivated aMPV does not induce protective immunity, reduce virus shedding, or result in decreased histopathologic lesions.

  8. Detection of infectious bronchitis virus 793B, avian metapneumovirus, Mycoplasma gallisepticum and Mycoplasma synoviae in poultry in Ethiopia.

    Science.gov (United States)

    Hutton, S; Bettridge, J; Christley, R; Habte, T; Ganapathy, K

    2017-02-01

    A survey was conducted into respiratory infectious diseases of poultry on a chicken breeder farm run by the Ethiopian Institute of Agricultural Research (EIAR), located in Debre Zeit, Ethiopia. Oropharyngeal swabs were collected from 117 randomly selected birds, and blood was taken from a subset of 73 of these birds. A combination of serological and molecular methods was used for detection of pathogens. For the first time in Ethiopia, we report the detection of variant infectious bronchitis virus (793B genotype), avian metapneumovirus subtype B and Mycoplasma synoviae in poultry. Mycoplasma gallisepticum was also found to be present; however, infectious laryngotracheitis virus was not detected by PCR. Newcastle disease virus (NDV) was not detected by PCR, but variable levels of anti-NDV HI antibody titres shows possible exposure to virulent strains or poor vaccine take, or both. For the burgeoning-intensive industry in Ethiopia, this study highlights several circulating infectious respiratory pathogens that can impact on poultry welfare and productivity.

  9. First Report of Avian Metapneumovirus Subtype B Field Strain in a Romanian Broiler Flock During an Outbreak of Respiratory Disease.

    Science.gov (United States)

    Franzo, Giovanni; Tucciarone, Claudia Maria; Enache, Mirel; Bejan, Violeta; Ramon, Gema; Koutoulis, Konstantinos C; Cecchinato, Mattia

    2017-06-01

    Avian metapneumovirus (aMPV) represents one of the most prevalent diseases of turkey, especially in combination with other pathogens, and its frequency is also increasing among chickens. Despite this evidence, epidemiologic data are poor and scattered, severely preventing control of the disease even in highly developed areas such as Europe. In the present study, the detection and characterization of an aMPV subtype B strain circulating in a vaccinated but symptomatic Romanian broiler flock is reported for the first time. The phylogenetic analysis based on the partial G gene sequence demonstrates the close relationship of the Romanian virus with a group of recently emerged Italian field strains for which vaccine-induced protection was experimentally proven to be partial. These preliminary results allow us to hypothesize the spreading of vaccine-escaping aMPV subtype B strains through Europe and, consequently, dictate the carrying out of a more systematic survey to confirm this theory and enforce adequate countermeasures.

  10. Viral replication and lung lesions in BALB/c mice experimentally inoculated with avian metapneumovirus subgroup C isolated from chickens.

    Science.gov (United States)

    Wei, Li; Zhu, Shanshan; She, Ruiping; Hu, Fengjiao; Wang, Jing; Yan, Xu; Zhang, Chunyan; Liu, Shuhang; Quan, Rong; Li, Zixuan; Du, Fang; Wei, Ting; Liu, Jue

    2014-01-01

    Avian metapneumovirus (aMPV) emerged as an important respiratory pathogen causing acute respiratory tract infection in avian species. Here we used a chicken aMPV subgroup C (aMPV/C) isolate to inoculate experimentally BALB/c mice and found that the aMPV/C can efficiently replicate and persist in the lungs of mice for at least 21 days with a peak viral load at day 6 postinoculation. Lung pathological changes were characterized by increased inflammatory cells. Immunochemical assay showed the presence of viral antigens in the lungs and significant upregulation of pulmonary inflammatory cytokines and chemokines including MCP-1, MIP-1α, RANTES, IL-1β, IFN-γ, and TNF-α were detected following inoculation. These results indicate for the first time that chicken aMPV/C may replicate in the lung of mice. Whether aMPV/C has potential as zoonotic pathogen, further investigation will be required.

  11. In-Vitro Antiviral Activities of Extracts of Plants of The Brazilian Cerrado against the Avian Metapneumovirus (aMPV

    Directory of Open Access Journals (Sweden)

    LK Kohn

    2015-09-01

    Full Text Available ABSTRACTAvian metapneumovirus (aMPV is a negative-sense single-stranded RNA enveloped virus of the Metapneumovirus genus belonging to theParamyxoviridae family. This virus may cause significant economic losses to the poultry industry, despite vaccination, which is the main tool for controlling and preventing aMPV. The aim of this study was to evaluate the antiviral activity of extracts of four different native plants of the Brazilian Cerrado against aMPV. The antiviral activity against aMPV was determined by titration. This technique measures the ability of plant extract dilutions (25 to 2.5 µg mL-1 to inhibit the cytopathic effect (CPE of the virus, expressed as inhibition percentage (IP. The maximum nontoxic concentration (MNTC of the extracts used in antiviral assay was 25 µg mL-1for Aspidosperma tomentosumand Gaylussacia brasiliensis, and 2.5 µg mL-1for Arrabidaea chicaand Virola sebifera. Twelve different extracts derived from four plant species collected from the Brazilian Cerrado were screened for antiviral activity against aMPV. G. brasiliensis, A. chica,and V. sebifera extracts presented inhibition rates of 99% in the early viral replication stages, suggesting that these extracts act during the adsorption phase. On the other hand, A. tomentosum inhibited 99% virus replication after the virus entered the cell. The biomonitored fractioning of extracts active against aMPV may be a tool to identify the active compounds of plant extracts and to determine their precise mode of action.

  12. Estudos experimentais com isolados do metapneumovirus aviário (aMPV) subtipos A e B em frangos de corte

    OpenAIRE

    Márcia Bianchi dos Santos

    2010-01-01

    Resumo: O Metapneumovirus aviário (aMPV) pertence à família Paramyxoviridae, subfamília Pneumovirinae, gênero Metapneumovirus. O vírus, relatado pela primeira vez no Brasil em 1995, é o agente etiológico da Rinotraqueíte em perus (TRT) e está associado também à Síndrome da Cabeça Inchada (SHS) em frangos e poedeiras comerciais. O presente estudo foi dividido em três partes. Na primeira foi avaliada a suscetibilidades de oito sistemas celulares para a propagação de amostras virais do aMPV subt...

  13. PARA UNA LECTURA CRÍTICA DEL DESARROLLO HUMANO

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    Melisa Campana

    2013-01-01

    Full Text Available El presente trabajo propone una lectura crítica del discurso del desarrollo humano articulado por Amartya Sen en Desarrollo y Libertad , desde el enfoque de la gubernamentalidad. En primer lugar, se precisa la perspectiva teórica adoptada, haciendo hincapié en las particularidades de la razón gubernamental neoliberal, marco en el que emerge el discurso del desarrollo humano. Se rastrea, luego, un conjunto de premisas del modelo de la elección racional, que brinda elementos conceptuales al discurso del desarrollo humano. A partir de estas claves, se examina una serie de postulados vertebradores de la propuesta de Amartya Sen, a los fines de identificar algunos de los efectos que ha producido el discurso del desarrollo humano en términos de concepción de la pobreza y de los dispositivos de intervención sobre ella.

  14. e implicaciones para el bienestar de los humanos

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    Germán Gutiérrez

    2007-01-01

    Full Text Available En este artículo se revisa la literatura reciente sobre interacciones humano-animal, su origen, sus características y cómo dichas interacciones afectan el bienestar físico, psicológico y social. En la primera parte se presentan los orígenes y evolución histórica de las relaciones humano - animal, empezando por los procesos de domesticación y se aborda la naturaleza de las interacciones humano animal. En la segunda parte, se revisan los efectos para los humanos de la interacción con animales, especialmente con sus mascotas y se analiza la literatura científica sobre los efectos físicos, psicológicos y sociales. Finalmente, se aborda brevemente el desarrollo de esta área de investigación en Latinoamérica.

  15. El recurso humano factor de competitividad en el sector salud

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    Remedios Piteres Redondo

    2017-10-01

    Full Text Available Objetivo: realizar un análisis del papel que juega la gestión del recurso humano al interior de una organización que busca la competitividad.Resultados y conclusiones: el talento humano de una empresa constituye el activo más valioso de la misma, es por esto que una buena gestión del talento humano se debe  caracterizar por potenciar el capital humano en beneficio de los objetivos, visualizando  a los empleados como socios para que a través de sus talentos desarrollen las  estrategias, antes que a través de acciones administrativas generando  una ventaja competitiva en el mercado e impactando  positivamente los diversos programas y políticas, así como los estados financieros de la empresa.

  16. Oxitocina e comportamento humano Oxitocina y comportamiento humano Oxytocin and human behavior

    Directory of Open Access Journals (Sweden)

    Diana Catarina Ferreira de Campos

    2010-07-01

    Full Text Available Recentemente, têm surgido numerosas especulações sobre o papel da oxitocina nas emoções e relações sociais do ser humano. O presente artigo retrata um estudo essencialmente reflexivo sobre o papel da oxitocina no comportamento humano. A pesquisa bibliográfica foi efectuada nas bases de dados MEDLINE, versão PubMed e CINAHL. Os principais resultados das pesquisas sustentam que os níveis de oxitocina aumentam em resposta à aplicação de massagem e indução de emoções positivas. A administração intranasal de oxitocina aumenta os níveis de calma, diminui o nível de ansiedade e promove comportamentos de confiança entre as pessoas. Uma melhor compreensão dos mecanismos neurobioquímicos e biológicos do comportamento humano têm implicações cruciais para o desenvolvimento do conhecimento científico sobre patologias psiquiátricas com especial handicap nas relações sociais (exemplo: autismo, esquizofrenia, personalidade borderline.Recientemente, han surgido numerosas especulaciones sobre el papel de la oxitocina en las emociones y relaciones sociales del ser humano. El presente artículo retrata un estudio esencialmente reflexivo sobre el papel de la oxitocina en el comportamiento humano. La investigación bibliográfica fue efectuada en las bases de datos MEDLINE, versión PubMed y CINAHL. Los principales resultados de la investigación sustentan que los niveles de oxitocina aumentan en respuesta a la aplicación del masaje e inducción de emociones positivas. La administración intranasal de oxitocina aumenta los niveles de tranquilidad, disminuye el nivel de ansiedad y promueve comportamientos de confianza entre las personas. Una mejor comprensión de los mecanismos neurobioquímicos y biológicos del comportamiento humano tiene implicaciones cruciales para el desarrollo del conocimiento científico sobre patologías psiquiátricas con especial handicap en las relaciones sociales (ejemplo: autismo, esquizofrenia

  17. Ciencia, desarrollo humano y calidad de vida

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    Rocío Calderón García

    2009-01-01

    Full Text Available En la sociedad contemporánea, la ciencia y la tecnología se hanconvertido en el centro alrededor del cual se entretejen las rela-ciones sociales y la vida social. La sociedad del conocimiento,como se le ha denominado al modelo de la sociedad a la que as-piraríamos, precisamente por el desarrollo que ha tenido el cono-cimiento tecnológico y cientíęco en estos momentos, se convierteen el ámbito natural para que el tema de la tecnociencia formeparte de los procesos formativos y constitutivos de las nuevasidentidades sociales. El hecho de entender como la poblaciónincorpora los conceptos de ciencia y tecnología en sus agendasde discusión en la toma de decisiones sobre su vida y en sus ac-tividades cotidianas, se convierte hoy en día en una explicaciónnecesaria para entender los procesos de formación y expresióndel ser humano contemporáneo.

  18. BUCLE ENTRE LAICIDAD Y DERECHOS HUMANOS

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    Felipe Gaytán Alcalá

    2014-06-01

    Full Text Available La laicidad como referente del espacio público y del marco jurídico de la ciudadanía se ha vuelto un tema de debate político en el mundo, particularmente en América Latina. Va más allá de la separación entre Estado e Iglesias y el discurso anticlerical. La Laicidad es un debate sobre los derechos y libertades civiles de los ciudadanos en el respeto de sus derechos humanos por parte de organizaciones, cultos, creencias religiosas. Diversos estudios se han impulsado en este marco de reflexión, estudios que transitan las fronteras entre lo académico, político, militancia social, etc. En el presente texto abordamos las diversas formas que el concepto ha tenido en AL y las implicaciones en la narrativa de los DH y la diversidad cultural. Particularmente analizaremos tres enfoques de la laicidad no excluyentes entres sí: uno de ellos refiere a la laicidad inclusiva en el espacio público, la segunda entiende la laicidad como garante de la autonomía de lo político marcando una exclusión entre creencia y ciudadanía y; la tercera, predominante en la región, subraya la autonomía del Estado frente a las organizaciones religiosas expresada en la separación Estado- Iglesias.

  19. Quilombolas e direitos humanos no Suriname

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    Richard Price

    1999-05-01

    Full Text Available Resumo Desde a independência do Suriname em 1975, o relacionamento entre o Estado e sua população quilombola (cerca de 52 mil pessoas, talvez 15% da população nacional deteriorou. Do ponto de vista quilombola, os tipos de atrocidades associadas às guerras coloniais do século dezoito voltaram à tona. A Guerra Civil que começou em 1986, que jogou o exército nacional contra os “Jungle Commandos” (compostos principalmente de quilombolas só fez piorar a situação dos quilombolas. Um grande julgamento pela Corte Inter-Americana de Direitos Humanos em 1992, tendo como réu o Estado do Suriname, terminou com uma vultosa indenização por danos aos quilombolas Saramaka, autores da queixa. Recentemente a situação piorou, quando os quilombolas – sem qualquer proteção legal sob a constituição do Suriname – viram as terras pelas quais os seus antepassados lutaram e morreram serem confiscadas unilateralmente pelo Estado e doadas, em concessões gigantescas, a companhias madeireiras e mineradoras multinacionais (da Indonésia, da Malásia, da China e do Canadá. O efeito das atuais políticas para os quilombolas não é nada menos do que etnocídio.

  20. Los derechos humanos en el franquismo

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    Josefa Dolores Ruiz Resa

    2014-06-01

    Full Text Available Durante el régimen franquista se reconocieron, en diversos textos jurídicos como el Fuero del Trabajo (1938 o el Fuero de los Españoles (1945, una serie de derechos. Entre ellos se encuentran, por ejemplo, el derecho al trabajo, a participar en las funciones públicas, entre otros. En este artículo se hace una revisión histórica con el objetivo de determinar sobre qué presupuestos ideológicos se basó la concepción de los derechos durante el franquismo, cómo se justificó su reconocimiento en una dictadura que se había declarado contraria a las doctrinas modernas que habían servido para reivindicar los derechos humanos (es decir, el constitucionalismo, el liberalismo político o la socialdemocracia, y con qué diseño y alcance legal fueron reconocidos aquellos derechos.

  1. POBREZA MUNDIAL, JUSTICIA Y DERECHOS HUMANOS

    Directory of Open Access Journals (Sweden)

    Mauricio Andrés Gallo Callejas

    2010-11-01

    Full Text Available En este escrito pregunto por el estatus normativo que, bajo nuestras creencias actuales, debemos dar a la situación de millones de personas condenadas a pasar una vida en condiciones de pobreza extrema alrededor de todo el planeta. Asunto frente al cual formulo las siguientes dos tesis: (i la construcción de juicios normativos que nos permitan redescribir el sentido valorativo que estamos dando a su situación no sólo es posible, sino que es nuestro deber. Tal redescripción significa incluir su dolor y sufrimiento dentro de los asuntos dejusticia política global que incumben a toda la comunidad de seres humanos. Y (ii dicha redescripción, o lo que es igual, otorgar este nuevo estatus normativo a la pobreza mundial no nos exige ni proponer la creación de instituciones que, como la república mundial o principios globales de justicia distributiva, ponganen peligro el valor de los actuales Estados nación, ni mucho menos, justificar la violencia como herramienta de lucha política en búsqueda de la corrección moralde dichas instituciones.

  2. Los derechos humanos y el medioambiente

    Directory of Open Access Journals (Sweden)

    Juan José Rodríguez Beltrán

    2006-01-01

    Full Text Available Los derechos humanos son individuales, esenciales, inalienables e inherentes a la dignidad de la persona humana, constituyen la base del Estado social de derecho, y son anteriores al derecho positivo. Por su parte, hay otro tipo de derechos que son los colectivos, los cuales radican en cabeza del colectivo social y están ligados a la calidad de vida de las personas. Existe una estrecha relación entre los unos y los otros, pero el interrogante radica en saber cuál prevalece; en la actualidad podría hablarse de una colisión o conflicto que se está presentando entre ambos, esto hace pensar hasta qué punto el crecimiento industrial y tecnológico del mundo es permisible en la medida en que se está comprometiendo el medioambiente y el equilibrio ecológico, lo cual va en detrimento de los derechos a la vida, a la salud y como fundamento último de la persona misma.

  3. É preciso salvar os direitos humanos!

    Directory of Open Access Journals (Sweden)

    José Augusto Lindgren Alves

    2012-01-01

    Full Text Available Os direitos humanos se encontram em fase de evidente desprestígio. Os motivos do descrédito não são apenas as violações que prosseguem, nem distorções decorrentes de valores não ocidentais. São também, e sobretudo, sua extensão conceitual exagerada, promovida pela esquerda culturalista pós-moderna, assim como a repetição inercial de ações e posturas, hoje anacrônicas, inerentes ao sistema de proteção internacional existente. Para recuperá-los em sentido construtivo é necessário voltar à Declaração Universal de 1948, atualizar o discurso originalmente construído contra ditaduras e readaptar o sistema à situação das democracias atuais ameaçadas.Human rights are now in an evident lackluster phase. Causes of disbelief in them are not only their continuing violations, nor distortions that stem from non-Western values. First and foremost, they involve an exaggerated conceptual extension of those rights, promoted by the post-modern cultural Left, as well as the endless repetition of today anachronistic forms of action and positions inherent to the existing international protection system. In order to recuperate human rights on a constructive direction it is necessary to return to the Universal Declaration, of 1948, to update Lua Nova, São Paulo, 86: 253-260, 2012 255 Resumos / Abstracts a discourse that was originally built against dictatorships, and to re-adapt the whole system to the situation of present democracies under threat.

  4. Defesa dos direitos humanos: sistemas regionais

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    Hélio Bicudo

    2003-04-01

    Full Text Available O texto discorre sobre a institucionalização dos sistemas de promoção dos Direitos Humanos, ao longo do século XX. Tomando a Declaração Universal dos Direitos do Homem, de 1948, como base e ponto de partida, o articulista aborda as três principais vertentes dos sistemas de promoção desses direitos: a européia, a americana e a africana. A evolução de cada uma delas descrita analógica e epistemologicamente, de forma a salientar singularidades sem perder de vista as influências recíprocas ou mesmo as determinantes próprias do contexto espaço-social. Para finalizar, o articulista destaca a importância do Tribunal Penal Internacional no estabelecimento de uma justiça ecumênica. Concomitantemente, alerta para o perigo contido na ameaça de os EUA não o ratificarem.The text underlines the institutional character acquired by the main systems to promote Human Rights in the twentieth century. Taking the Universal Human Rigths Declaration, of 1948, as the basis for analysis, the author brings up three different approaches to promote these rights: the European, the American and the African. The evolution of each of them is described using its procedure as a basic reference. Besides, an analogical analysis, brings to the fore singularities, without ignoring reciprocal influences dictated by particular contexts, though. The author stresses the importance of the International Penal Court, as a means to establish ecumenical justice. At the same time, he reminds the reader of the risks implicit in the possible non-ratification of the IPC by United States.

  5. Direitos humanos na atualidade: um mundo em crise

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    Eduardo Gomes Biacchi

    2017-04-01

    Full Text Available Preocupante é a ascensão do populismo dentro dos Estados Democráticos de Direito, em uma clara afronta e violação aos direitos humanos. Direitos esses que foram arduamente construídos e conquistados pela humanidade ao longo de períodos históricos e tiveram como ápice o fim da Segunda Guerra Mundial e a construção do Sistema Global de Proteção por meio da Organização das Nações Unidas, no século XX. No século XXI, diferentemente do que imaginávamos, caminhamos para um cenário confuso e melancólico, ante as claras e manifestas violações aos direitos humanos por parte dos Estados. Não obstante a comunidade internacional questione a efetividade na tutela dos direitos humanos dentro da ONU, em virtude da inexistência de mecanismos institucionalizados para buscarem a responsabilização internacional do Estado contra graves violações aos direitos humanos, a exemplo do que ocorre dentro do Sistema Interamericano de Proteção aos Direitos Humanos, verificamos que, guardadas as devidas proporções e dentro dos limites de atuação que o próprio direito internacional impõe, a ONU procura atuar eficazmente. [continua

  6. Gestão de pessoas: de recursos humanos ao processo de desenvolvimento humano e social

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    Carla Cristina Dutra Búrigo

    2011-01-01

    Full Text Available Este ensaio teórico-empírico tem como objetivo possibilitar reflexões sobre o processo de constituição da área de Gestão de Pessoas no que tange a concepção de homem, de mundo e de sociedade, revelando a possibilidade de elevar o processo de gerenciamento de recursos humanos ao de desenvolvimento humano e social. Para tanto, situamos como realidade concreta para o estudo neste ensaio, a área de Gestão de Pessoas da Universidade Federal de Santa Catarina (UFSC, no que se refere a sua ação como unidade administrativa diante da política de desenvolvimento e do contexto no qual está inserida. A Pró-Reitoria de Desenvolvimento Humano e Social (PRDHS/UFSC é um órgão executivo da administração superior, criada com o objetivo de auxiliar o Reitor em suas tarefas na área de Gestão de Pessoas, com vistas à melhoria do nível de qualidade de vida no trabalho do servidor3, a sua potencialização como agente de transformação social e a humanização das relações de trabalho. Concebemos que a área de Gestão de Pessoas é um desafio lógico. É viver entre a utopia e a realidade cada vez mais estreita e sufocante, e das contradições e antagonismos do que é necessário fazer e o que verdadeiramente se faz diante da tônica do desenvolvimento.Abstract This theoretical and empirical essay aims to discuss the process of establishing the area of People Management in relation to conception of man, world and society, revealing the possibility of raising the process of managing the human resources to the human and social development. Therefore, we place as a concrete reality to study this essay, the area of PeopleManagement from the Federal University of Santa Catarina (UFSC, in relation to its action as an administrative unit on its development policy and the context in which it is inserted. The Pró-Reitoria de Desenvolvimento Humano e Social (PRDHS/UFSC is an executive body ofsenior management, created with the objective of

  7. Proteção internacional dos direitos humanos

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    Amanda Thereza Lenci Paccola

    2017-11-01

    Full Text Available Podemos observar no presente trabalho de pesquisa, desde as bases dos Direitos Humanos, suas influências e surgimento. Esta pesquisa tende a expor como funcionam seus mecanismos e quais são os seus procedimentos, com grande enfoque na atuação da Organização das Nações Unidas (ONU. Os direitos humanos muitas vezes, apesar de protegidos por tratados internacionais não são respeitados. Assim, nesta abordagem, veremos os principais pactos/tratados/convenções sobre o tema, e como proposito final será feita uma análise de como a diplomacia e o governo brasileiro se comportam em relação à proteção do instituto de direitos humanos, juntamente com a importância dada a ele atualmente em sua política externa.

  8. OPTIMIZACIÓN DEL CAPITAL HUMANO VERSUS DESEMPLEO

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    Armando Cuesta Santos

    2003-12-01

    Full Text Available

    Es argumentada la necesidad de trascender los paradigmas de considerar al trabajador como un costo y también de concebirlo como una inversión de la empresa, asumiendo el paradigma de considerarlo como inversión de su propio capital humano. En consecuencia, el enfoque reductor de personal con el consiguiente desempleo en aras del incremento de la productividad del trabajo, deberá ser superado por el enfoque de optimización del capital humano en la organización. El objetivo de este trabajo es contribuir a esa optimización al ir asumiendo el paradigma del trabajador como inversor de su capital humano, en oposición al desempleo implicado por el paradigma predominante concibiendo al trabajador como un costo.

  9. Jurisprudencia de la Corte Interamericana de Derechos Humanos

    OpenAIRE

    Salmón, Elizabeth

    2011-01-01

    La valiosa Jurisprudencia de la Corte Interamericana de Derecho. Humanos no agota sus efectos en sus beneficiarios directos sino que también favorece al conjunto de la población de lo: estados sometidos a su jurisdicción al precisar el sentido contenido y alcances de los derechos. Esta función preventiva según se demuestra en esta publicación, constituye una finalidad esencial de todo sistema internacional de protección de derechos humanos. En este libro se identifica y analiza algunos de los...

  10. Sentencia sobre Reparación Integral de Derechos Humanos

    OpenAIRE

    Ecuador. Consejo de la Judicatura

    2014-01-01

    El Programa Andino de Derechos Humanos publica a continuación la sentencia emitida por un Juez de Tránsito en la que se determina la reparación integral para los familiares de una persona muerta a causa de un accidente de tránsito. Constituye un precedente importante en especial por la aplicación de estándares internacionales sobre la reparación integral de derechos humanos en cumplimiento con la normativa internacional.

  11. Derechos Humanos y Políticas Públicas. Manual

    OpenAIRE

    Deluchey, Jean-François Y.; Alza Barco, Carlos; Urueña, René; Montoya Céspedes, Nicolás; Estupiñan Silva, Rosmerlin; Fernández Gómez, Itziar; Sánchez Mojica, Beatriz Eugenia; Gavazzo, Natalia; Aguiar de Luque, Luis; Alencar Mayer Feitosa, María Luiza; Bregaglio, Renata; Urueña, René; Sánchez, Beatriz Eugenia; Anzola, Sergio; Salmón, Elizabeth

    2014-01-01

    El presente manual, el tercero y último elaborado en el marco de la Red Derechos Humanos y Educación Superior, tiene por objetivo ilustrar las relaciones que se establecen entre los derechos humanos y las políticas públicas. Se trata de un vínculo bastante más complejo de lo que a primera vista podría parecer. En efecto, los primeros no sólo son objeto de las segundas, como estrategia para alcanzar su garantía. También se han convertido en elementos centrales en el diseño...

  12. Grupo de sala de espera: sentidos do envelhecimento humano

    OpenAIRE

    Cesar Sandoval Moreira Jr.; Marisa Japur

    2003-01-01

    Este trabalho parte da consideração do envelhecimento humano como experiência heterogênea. Busca pôr em diálogo o grupo de sala de espera, recorrendo ao construcionismo social, para descrevê-lo como prática discursiva. Problematizando essa modalidade de intervenção psicológica, nesse estudo objetivamos descrever o processo de produção de sentidos sobre o envelhecimento humano nesse contexto. Dez grupos de sala de espera, gravados e transcritos, foram pré-analisados favorecendo a escolha de um...

  13. Competencias laborales de los gerentes de talento humano

    OpenAIRE

    Gregorio Calderón Hernández; Julia Clemencia Naranjo Valencia

    2004-01-01

    El reconocimiento de la importancia de las personas en el desarrollo de las organizaciones, especialmente por la posibilidad de construir ventaja competitiva fundamentada en el talento humano, está demandando de quienes lideran el área de recursos humanos el desarrollo de competencias que respondan a los nuevos retos. La revisión de investigaciones realizadas sobre el tema en otras latitudes permite concluir que existen diferentes tipologías de competencias para los profesionales del área de ...

  14. Selección de Recursos Humanos por Competencias

    OpenAIRE

    Sánchez Domingo, Cristina

    2013-01-01

    Los objetivos de este trabajo son, por un lado conocer en qué consisten los procesos de valoración y selección de personas desde el enfoque de las competencias laborales. Para ello, ha sido preciso profundizar en el estudio del concepto de “competencia laboral”, y comprender qué es la Gestión de Recursos Humanos por Competencias, en cuyo seno se encuentra la propia Selección de Recursos Humanos. Por otra parte, un segundo objetivo del trabajo es conocer el grado de implantación de la Selecció...

  15. Parasitismo de Amblyomma fuscum (Acari: Ixodidae) em humanos

    OpenAIRE

    Marques Sandro; Dal Col Rogério; Matos Júnior Marco Otávio de; Gonçalves Elisabeth Fernandes Bertoletti; Pinter Adriano; Labruna Marcelo Bahia

    2006-01-01

    Amblyomma fuscum é uma espécie rara de carrapato, recentemente revalidada, com poucos registros de ocorrência na literatura. Neste trabalho, são informados dois registros de parasitismo de A. fuscum em seres humanos: um no Estado de São Paulo (SP) e outro possivelmente oriundo do Estado de Santa Catarina (SC), Brasil. Tratam-se dos primeiros registros de parasitismo em humano por esta espécie de carrapato. Um espécime adulto fêmea de A. fuscum foi coletado quando encontrava-se fixado à palma ...

  16. Microbioma humano: implicações biomédicas

    OpenAIRE

    Correia, Filipe André Saraiva

    2015-01-01

    Dissertação para obtenção do grau de Mestre no Instituto Superior de Ciências da Saúde Egas Moniz O microbioma humano é o conjunto dos microrganismos presentes no organismo, sendo estes microrganismos caracterizados através da análise qualitativa do seu genoma. O desenvolvimento de técnicas analíticas inovadoras permitiu a identificação e compreensão das funções do microbioma humano. Este desempenha importantes funções metabólicas e influencia o funcionamento do sistema imunitário. O micro...

  17. DIREITOS HUMANOS, DESENVOLVIMENTO SUSTENTÁVEL E SUSTENTABILIDADE

    OpenAIRE

    Carvalho, Sonia Aparecida de; Silva, Denival Francisco da; Adolfo, Luiz Gonzaga Silva

    2015-01-01

    Este artigo trata da relação intrínseca entre os direitos humanos, o desenvolvimento sustentável e a sustentabilidade. Seu objetivo geral consiste em analisar o modelo de desenvolvimento implementado e a necessária proteção e preservação do meio ambiente ligado à defesa dos direitos humanos. Os objetivos específicos pretendem investigar a distinção dos termos sustentabilidade e desenvolvimento sustentável, como a concretização do direito à sustentabilidade, os critérios e as dimensões da sust...

  18. Jurisprudencia social del Tribunal Europeo de Derechos Humanos

    OpenAIRE

    Martínez Miranda, Macarena

    2016-01-01

    El presente artículo pretende reflexionar sobre la incidencia de la Jurisprudenciadel Tribunal Europeo de Derechos Humanos en el ámbito laboral, particularmenteen materia de derechos fundamentales, como canon hermenéutico de indudablevalor, al formar un cuerpo de doctrina relativa al Convenio Europeo de DerechosHumanos, así como a otros instrumentos emanados de órganos del Consejo deEuropa; con especial énfasis en determinados pronunciamientos, sin ánimoexhaustivo, tanto en el ámbito de propi...

  19. In vitro antiviral activity of Brazilian plants (Maytenus ilicifolia and Aniba rosaeodora) against bovine herpesvirus type 5 and avian metapneumovirus.

    Science.gov (United States)

    Kohn, L K; Queiroga, C L; Martini, M C; Barata, L E; Porto, P S S; Souza, L; Arns, C W

    2012-10-01

    Medicinal plants are well known for their use in traditional folk medicine as treatments for many diseases including infectious diseases. Six Brazilian medicinal plant species were subjected to an antiviral screening bioassay to investigate and evaluate their biological activities against five viruses: bovine herpesvirus type 5 (BHV-5), avian metapneumovirus (aMPV), murine hepatitis virus type 3, porcine parvovirus and bovine respiratory syncytial virus. The antiviral activity was determined by a titration technique that depends on the ability of plant extract dilutions (25 or 2.5 µg/mL) to inhibit the viral induced cytopathic effect and the extracts' inhibition percentage (IP). Two medicinal plant species showed potential antiviral activity. The Aniba rosaeodora Ducke (Lauraceae) extract had the best results, with 90% inhibition of viral growth at 2.5 µg/mL when the extract was added during the replication period of the aMPV infection cycle. The Maytenus ilicifolia (Schrad.) Planch. (Celastraceae) extracts at a concentration of 2.5 µg/mL exhibited antiviral activity during the attachment phase of BHV-5 (IP = 100%). The biomonitored fractionation of the active extracts from M. ilicifolia and A. rosaeodora could be a potential tool for identifying their active compounds and determining the exact mechanism of action.

  20. Detection by reverse transcriptase-polymerase chain reaction and molecular characterization of subtype B avian metapneumovirus isolated in Brazil.

    Science.gov (United States)

    Chacón, Jorge Luis; Brandão, Paulo E; Buim, Marcos; Villarreal, Laura; Ferreira, Antonio J Piantino

    2007-10-01

    Subtype B avian metapneumovirus (aMPV) was isolated and detected by reverse transcriptase-polymerase chain reaction (RT-PCR) in Brazilian commercial laying chicken flocks with no history of vaccination against aMPV and presenting respiratory signs and decreased egg production. RT-PCR results from samples from three affected flocks revealed that the three isolates were subtype B. Partial sequence analysis of the G glycoprotein gene confirmed that the samples belonged to subtype B and were not of the vaccine type. Comparison of nucleotide and amino acid sequences of the G gene of the three Brazilian aMPV samples with subtype B isolates from other countries revealed 95.1% to 96.1% identity. Nucleotide sequences showed 100% identity among the Brazilian subtype B samples and 95.6% identity with the subtype B vaccine strain used in Brazil. This work describes the circulation of subtype B aMPV in Brazil and discusses its importance in terms of disease epidemiology.

  1. A sensitive, reproducible, and economic real-time reverse transcription PCR detecting avian metapneumovirus subtypes A and B.

    Science.gov (United States)

    Franzo, G; Drigo, M; Lupini, C; Catelli, E; Laconi, A; Listorti, V; Bonci, M; Naylor, C J; Martini, M; Cecchinato, M

    2014-06-01

    Use of real-time PCR is increasing in the diagnosis of infectious disease due to its sensitivity, specificity, and speed of detection. These characteristics make it particularly suited for the diagnosis of viral infections, like avian metapneumovirus (AMPV), for which effective control benefits from continuously updated knowledge of the epidemiological situation. Other real-time reverse transcription (RT)-PCRs have been published based on highly specific fluorescent dye-labeled probes, but they have high initial cost, complex validation, and a marked susceptibility to the genetic variability of their target sequence. With this in mind, we developed and validated a SYBR Green I-based quantitative RT-PCR for the detection of the two most prevalent AMPV subtypes (i.e., subtypes A and B). The assay demonstrated an analytical sensitivity comparable with that of a previously published real-time RT-PCR and the ability to detect RNA equivalent to approximately 0.5 infectious doses for both A and B subtypes. The high efficiency and linearity between viral titer and crossing point displayed for both subtypes make it suited for viral quantification. Optimization of reaction conditions and the implementation of melting curve analysis guaranteed the high specificity of the assay. The stable melting temperature difference between the two subtypes indicated the possibility of subtyping through melting temperature analysis. These characteristics make our assay a sensitive, specific, and rapid tool, enabling contemporaneous detection, quantification, and discrimination of AMPV subtype A and B.

  2. Avian metapneumovirus M2:2 protein inhibits replication in Vero cells: modification facilitates live vaccine development.

    Science.gov (United States)

    Clubbe, Jayne; Naylor, Clive J

    2011-11-28

    Throughout the world, avian metapneumovirus (AMPV) infection of subtype A is principally controlled by two live vaccines both derived from UK field strain #8544. Improvements of those vaccines by use of reverse genetics technology was found to be hampered by the inability of #8544 to replicate in the commonly exploited Vero cell based reverse genetics system. A systematic reverse genetics based genome modification of a DNA copy of #8544, employing sequence data from a Vero grown, #8544 derived, live vaccine; was used to determine mutations required to facilitate virus recovery and replication in Vero cells. This identified a single coding substitution in the M2:2 reading frame as responsible. Furthermore, ablation of M2:2 was found to elicit the same outcome. M2:2 sequence analysis of seven AMPVs found Vero cell adaption to be associated with non similar amino acid changes in M2:2. The study shows that M2:2 modification of field virus #8544 will enable research leading to improved vaccines. This may have more general application to other AMPV field strains. Copyright © 2011 Elsevier Ltd. All rights reserved.

  3. A single polymerase (L) mutation in avian metapneumovirus increased virulence and partially maintained virus viability at an elevated temperature.

    Science.gov (United States)

    Brown, Paul A; Lupini, Caterina; Catelli, Elena; Clubbe, Jayne; Ricchizzi, Enrico; Naylor, Clive J

    2011-02-01

    Previously, a virulent avian metapneumovirus, farm isolate Italy 309/04, was shown to have been derived from a live vaccine. Virulence due to the five nucleotide mutations associated with the reversion to virulence was investigated by their addition to the genome of the vaccine strain using reverse genetics. Virulence of these recombinant viruses was determined by infection of 1-day-old turkeys. Disease levels resulting from the combined two matrix mutations was indistinguishable from that produced by the recombinant vaccine, whereas the combined three L gene mutations increased disease to a level (P<0.0001) that was indistinguishable from that caused by the revertant Italy 309/04 virus. Testing of the L mutations individually showed that two mutations did not increase virulence, while the third mutation, corresponding to an asparagine to aspartic acid substitution, produced virulence indistinguishable from that caused by Italy 309/04. In contrast to the vaccine, the virulent mutant also showed increased viability at temperatures typical of turkey core tissues. The notion that increased viral virulence resulted from enhanced ability to replicate in tissues away from the cool respiratory tract, cannot be discounted.

  4. Avian metapneumovirus SH gene end and G protein mutations influence the level of protection of live-vaccine candidates.

    Science.gov (United States)

    Naylor, Clive J; Ling, Roger; Edworthy, Nicole; Savage, Carol E; Easton, Andrew J

    2007-06-01

    A prototype avian metapneumovirus (AMPV) vaccine (P20) was previously shown to give variable outcomes in experimental trials. Following plaque purification, three of 12 viruses obtained from P20 failed to induce protection against virulent challenge, whilst the remainder retained their protective capacity. The genome sequences of two protective viruses were identical to the P20 consensus, whereas two non-protective viruses differed only in the SH gene transcription termination signal. Northern blotting showed that the alterations in the SH gene-end region of the non-protective viruses led to enhanced levels of dicistronic mRNA produced by transcriptional readthrough. A synthetic minigenome was used to demonstrate that the altered SH gene-end region reduced the level of protein expression from a downstream gene. The genomes of the remaining eight plaque-purified viruses were sequenced in the region where the P20 consensus sequence differed from the virulent progenitor. The seven protective clones were identical, whereas the non-protective virus retained the virulent progenitor sequence at two positions and contained extensive alterations in its attachment (G) protein sequence associated with a reduced or altered expression pattern of G protein on Western blots. The data indicate that the efficacy of a putative protective vaccine strain is affected by mutations altering the balance of G protein expression.

  5. LOS EXILIADOS. LA LUCHA POR LOS DERECHOS HUMANOS DURANTE LA DICTADURA, de Silvina Jensen

    Directory of Open Access Journals (Sweden)

    Nicolás Hochman

    2010-04-01

    Full Text Available RESEÑASLOS EXILIADOS. LA LUCHA POR LOSDERECHOS HUMANOS DURANTE LA DICTADURAJENSEN, Silvina (2009. Los exiliados. La lucha por losderechos humanos durante la dictadura , Buenos Aires:Sudamericana. ISSN 978-950-073-158-4

  6. El coleccionismo: el rescate de las cosas y lo humano = Colecionismo: o resgate das coisas e do humano

    Directory of Open Access Journals (Sweden)

    Valis, Noël

    2013-01-01

    Full Text Available Proposta de uma reflexão aprofundada sobre o ato de colecionar, uma das pulsões menos compreendidas pelo pensamento contemporâneo, aqui concebido como uma extensão humanizadora do Ser. O destino dos objetos é o nosso destino, porque sem eles não existiríamos como seres verdadeiramente humanos. O artigo se apóia em teóricos como Walter Benjamin ou Hannah Arendt e se detêm em romancistas que têm refletido sobre o colecionismo em suas distopias, em que o ser humano é tratado como um objeto

  7. El reconocimiento del derecho humano a la verdad en El Salvador.

    OpenAIRE

    Arévalo Romero, Leonor Elisa; Reyes Reyes, Gertrudis Ernestina; Trejo Quintana, Densy Samuel

    2009-01-01

    Noción histórica del derecho humano a la verdad -- La idea de la verdad -- El reconocimiento del derecho humano a la verdad en El Salvador -- Marco constitucional y legal de El Salvador en materia del derecho humano a la verdad.

  8. Los derechos humanos laborales en el Derecho Internacional

    Directory of Open Access Journals (Sweden)

    Miguel Canessa Montejo

    2009-12-01

    Full Text Available El presente estudio pretende abordar esa temática desde el horizonte del Derecho internacional. En el primer acápite se plantea un concepto para este selecto grupo de derechos laborales básicos, así como se postulan los argumentos que justifican su reconocimiento jurídico en los instrumentos internacionales de derechos humanos. En el segundo acápite se aborda la identificación de los derechos laborales con los derechos sociales, como una forma de desvalorizar su relevancia jurídica. En el tercer acápite se resaltan las singularidades de los derechos humanos laborales, lo que dificulta someterlos a la clásica división entre derechos civiles y derechos sociales. En el cuarto acápite se establecen las obligaciones internacionales que se desprenden de los derechos humanos laborales hacia los Estados. Asimismo, se hace un breve recorrido sobre los procedimientos internacionales de control y los más importantes pronunciamientos de protección de los derechos humanos laborales. Finalmente, en el último acápite se presenta el jus cogens laboral como pilar del Derecho internacional del trabajo.

  9. Competencias laborales de los gerentes de talento humano

    Directory of Open Access Journals (Sweden)

    Gregorio Calderón Hernández

    2004-01-01

    Full Text Available El reconocimiento de la importancia de las personas en el desarrollo de las organizaciones, especialmente por la posibilidad de construir ventaja competitiva fundamentada en el talento humano, está demandando de quienes lideran el área de recursos humanos el desarrollo de competencias que respondan a los nuevos retos. La revisión de investigaciones realizadas sobre el tema en otras latitudes permite concluir que existen diferentes tipologías de competencias para los profesionales del área de recursos humanos, que no existen estudios específicos de competencias para los gerentes del área y que se puede construir un modelo de competencias a través de análisis cualitativos. Estas consideraciones motivaron la presente investigación, que contó con el apoyo de la Asociación Colombiana de Gestión Humana (ACRIP. Se utilizó una metodología hermenéutica mediante la aplicación de entrevistas de eventos conductuales y de mapa funcional. Los resultados se reflejan en un modelo que comprende cuatro categorías y 18 competencias, además de los valores de los gerentes de talento humano y el diccionario de competencias.

  10. Skinner: sobre ciência e comportamento humano

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    Angelo Augusto Silva Sampaio

    Full Text Available Atualmente, não se pode pensar no estudo do comportamento humano sem considerar a abordagem científica a este objeto: o campo da Psicologia científica. Esse campo é disputado por diversos enfoques teóricos que divergem quanto ao modo como definem ciência e comportamento humano. A abordagem de B. F. Skinner foi bastante proeminente no século XX, mas ainda continua a ser mal-entendida. Partindo do desenvolvimento histórico de sua obra, o presente texto visa a iluminar alguns aspectos relacionados às noções de ciência e comportamento humano desse autor e ressaltar as transformações por que passaram. Analisam-se três tópicos da obra de Skinner: seu período inicial (de 1930 a cerca de 1938, a obra "Ciência e Comportamento Humano" de 1953, e as influências da Biologia. São enfatizados aspectos relevantes da sua teorização sobre o tema: busca por relações funcionais, ênfase nos dados empíricos, operacionismo, externalismo, multideterminação do comportamento, experimentalismo, previsão e controle, ética.

  11. Direitos Humanos e Relações Internacionais

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    Valerio de Oliveira Mazzuoli

    2011-09-01

    Full Text Available O presente artigo tem como tema a incorporação das normas internacionais de proteção dos direitos humanos no ordenamento brasileiro, e os avanços tomados pelo judiciário brasileiro nesse sentido.

  12. Soberania e Direitos Humanos: dois conceitos irreconciliáveis

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    Valerio de Oliveira Mazzuoli

    2011-09-01

    Full Text Available O presente artigo busca analisar a relação contemporânea entre os Estados Nacionais e a proteção internacional dos Direitos Humanos, tendo como foco as questões de soberania.

  13. Relaciones entre el arte y los derechos humanos

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    Yolanda Sierra León

    2014-07-01

    Full Text Available Este artículo se propone reflexionar sumariamente acerca de la vinculación entre el arte y los derechos humanos, teniendo en cuenta el origen o proveniencia de las manifestaciones artísticas. Para el efecto, se consideran tres tipos de obras: 1. Obras originadas en una decisión judicial o estatal como medio de reparación a las víctimas; 2. Obras de arte provenientes de la propia iniciativa de los artistas, y 3. Obras o prácticas artísticas que realizan las víctimas que han sufrido violaciones a sus derechos humanos. Este texto parte de considerar que las obras de arte o las prácticas artísticas, relacionadas con los derechos humanos, tienen objetivos diferentes según sea su origen. Se plantea que el fin del Estado es reparar a las víctimas y contribuir con la garantía de no repetición; mientras que el fin de los artistas es manifestar su sensibilidad en relación con un tema específico que les interesa abordar; y para las víctimas, son mecanismos de resistencia y denuncia que permiten pasar del trauma individual a la conformación de sujetos colectivos con capacidad para incidir en las condiciones sociales que generan la violación a sus derechos humanos. Para pensar el tema, se abordan algunas sentencias del Sistema Interamericano de Derechos Humanos, y obras y prácticas artísticas de víctimas del conflicto armado colombiano.

  14. Protection by recombinant Newcastle disease viruses (NDV) expressing the glycoprotein (G) of avian metapneumovirus (aMPV) subtype A or B against challenge with virulent NDV and aMPV

    Science.gov (United States)

    Avian metapneumovirus (aMPV) and Newcastle disease virus (NDV) are threatening avian pathogens that cause sporadic but serious respiratory diseases in poultry worldwide. Although, vaccination, combined with strict biosecurity practices, has been the recommendation for controlling these diseases in t...

  15. Evaluation of a LaSota strain-based recombinant Newcastle disease virus (NDV) expressing the glycoprotein (G) of avian metapneumovirus (aMPV) subgroup A or B as a bivalent vaccine in turkeys

    Science.gov (United States)

    To develop a bivalent vaccine candidate, a LaSota strain-based recombinant Newcastle disease virus (NDV) clone expressing the glycoprotein (G) of avian metapneumovirus (aMPV) subgroup A or B was generated using reverse genetics. Vaccination of turkeys with the NDV/aMPV-A G or NDV/aMPV-B G recombinan...

  16. Generation and evaluation of recombinant Newcastle disease viruses (NDV) expressing the F and G proteins of avian metapneumovirus subtype C (aMPV-C) as bivalent vaccine against NDV and aMPV challenges in turkeys

    Science.gov (United States)

    Previously we generated a Newcastle disease virus (NDV) LaSota strain-based recombinant virus expressing the glycoprotein (G) of avian metapneumovirus subgroup C (aMPV-C) as a bivalent vaccine, which provided a partial protection against aMPV-C challenge in turkeys. To improve the vaccine efficacy,...

  17. Generation and evaluation of a recombinant Newcastle disease virus (NDV) expressing the F and G proteins of avian metapneumovirus subtype C (aMPV-C) as a bivalent vaccine against NDV and aMPV-C challenges in turkeys

    Science.gov (United States)

    Virulent strains of Newcastle disease virus (NDV) and avian metapneumovirus (aMPV) can cause serious respiratory diseases in poultry. Vaccination combined with strict biosecurity practices has been the recommendation for controlling NDV and aMPV diseases in the field. Previously we generated a NDV r...

  18. Ensaio sobre desenvolvimento humano na pós-modernidade Ensayo sobre desarrollo humano en la pos-modernidad Essay on human development in the post-modernity

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    José Antônio Damásio Abib

    2008-09-01

    Full Text Available Neste ensaio, o conceito de desenvolvimento humano na pós-modernidade apóia-se em uma filosofia pós-moderna de direitos humanos, e na modernidade apóia-se em uma filosofia moderna de direitos humanos. O Estado moderno fracassou na realização da filosofia moderna dos direitos humanos. Uma filosofia pós-moderna de direitos humanos deixa para trás a filosofia moderna de direitos humanos. Isso significa abandonar o fundacionismo ético e as grandes narrativas emancipatórias de desenvolvimento humano. Sugerimos que uma ética pragmatista da alteridade e uma política pós-moderna da alteridade podem contribuir para elaborar uma filosofia pós-moderna de direitos humanos.En este ensayo, el concepto de desarrollo humano en el pos-modernidad es apoyado en una filosofía pos-moderna de los derechos humanos y en la modernidad, es apoyado en una filosofía moderna de los derechos humanos. El Estado moderno fracasó en la realización de la filosofía moderna de los derechos humanos. Una filosofía pos-moderna de derechos humanos deja atrás la filosofía moderna de derechos humanos. Lo que significa abandonar el fundacionismo ético y las grandes narrativas emancipatorias del desarrollo humano. Sugerimos que una ética pragmatista de la alteridad y una política pos-moderna de alteridad pueden contribuir en la elaboración de una filosofía pos-moderna de derechos humanos.Whereas the concept of human development in post-modernity is supported by a post-modern philosophy of human rights, in modernity it is supported by a modern philosophy of human rights. The modern State has failed in the modern philosophy of human rights and the post-modern philosophy of human rights has laid aside a modern philosophy of human rights. This means that ethical foundationalism and emancipatory great narratives of human development have been discarded. We suggest that a pragmatist ethics of alterity and a post-modern policy of alterity may be a help in the

  19. Longitudinal field studies of avian metapneumovirus and turkey hemorrhagic enteritis virus in turkeys suffering from colibacillosis associated mortality.

    Science.gov (United States)

    Giovanardi, Davide; Lupini, Caterina; Pesente, Patrizia; Rossi, Giulia; Ortali, Giovanni; Catelli, Elena

    2014-06-01

    The aim of this study was to evaluate if the exposure to Avian metapneumovirus (aMPV) and/or to Turkey hemorrhagic enteritis virus (THEV) was significant for the induction of episodes of colibacillosis in aMPV and THEV vaccinated turkeys. Colibacillosis-associated mortality was recorded and longitudinal virological studies performed in three consecutive turkey flocks reared in the same farm. aMPV and THEV diagnostic swabs and blood samples were made once a week up to 14 weeks of age. Swabs were processed by molecular techniques for viruses detection and antibody titres were evaluated. Field subtype B aMPVs were detected in all flocks at different ages of life always associated with respiratory signs and increase of colibacillosis-associated mortality. THEV has been consistently detected in all flocks since the 9th week of age. Vaccination with a single dose of the THEV commercial inactivated vaccine available in Italy seems does not protect the birds from the infection. Sequence comparison of the hexon protein of one of the THEV strains detected, and strains isolated worldwide, revealed high similarity between them. These results are consistent with the notion that the hexon protein, being the major antigenic component of the virus, is highly conserved between the strains. Results showed that field aMPV infection is directly correlated to colibacillosis-associated mortality. Less clear appears the role of THEV because the endemicity of aMPV makes difficult to evaluate its role in predisposing colibacillosis in absence of aMPV. It would be interesting to further investigate this issue through experimental trials in secure isolation conditions.

  20. Deletion of the M2-2 gene from avian metapneumovirus subgroup C impairs virus replication and immunogenicity in Turkeys.

    Science.gov (United States)

    Yu, Qingzhong; Estevez, Carlos N; Roth, Jason P; Hu, Haixia; Zsak, Laszlo

    2011-06-01

    The second matrix (M2) gene of avian metapneumovirus subgroup C (aMPV-C) contains two overlapping open reading frames (ORFs), encoding two putative proteins, M2-1 and M2-2. Both proteins are believed to be involved in viral RNA transcription or replication. To further characterize the function of the M2-2 protein in virus replication, the non-overlapping region of the M2-2 ORF was deleted from an infectious cDNA clone of the aMPV-C strain, and a viable virus was rescued by using reverse genetics technology. The recombinant virus, raMPV-C ΔM2-2, was characterized in vitro and in vivo. In Vero cells, raMPV-C ΔM2-2 replicated slightly less efficiently than the parental virus, 10-fold reduction at 48-h post-infection. The raMPV-C ΔM2-2 virus induced typical cytopathic effects (CPE) that were indistinguishable from those seen with the parental virus infection. In specific-pathogen-free (SPF) turkeys, raMPV-C ΔM2-2 was attenuated and caused no clinical signs of disease. Less than 20% of the inoculated birds shed detectable virus in tracheal tissue during the first 5 days post-infection, and no virus shedding was detected afterward. Forty percent of infected birds produced a weak antibody response at 14 days post-infection. Upon challenge with a virulent aMPV-C strain, more than 80% of the raMPV-C ΔM2-2-inoculated birds showed typical disease signs and virus shedding in tracheal tissue. These results suggest that the M2-2 protein of aMPV-C virus is not essential for virus replication in vitro, but is required for sufficient virus replication to maintain pathogenicity and immunogenicity in the natural host.

  1. Evidence of avian metapneumovirus subtype C infection of wild birds in Georgia, South Carolina, Arkansas and Ohio, USA.

    Science.gov (United States)

    Turpin, E A; Stallknecht, D E; Slemons, R D; Zsak, L; Swayne, D E

    2008-06-01

    Metapneumoviruses (MPVs) were first reported in avian species (aMPVs) in the late 1970s and in humans in 2001. Although aMPVs have been reported in Europe and Asia for over 20 years, the virus first appeared in the United States in 1996, leaving many to question the origin of the virus and why it proved to be a different subtype from those found elsewhere. To examine the potential role of migratory waterfowl and other wild birds in aMPV spread, our study focused on determining whether populations of wild birds have evidence of aMPV infection. Serum samples from multiple species were initially screened using a blocking enzyme-linked immunosorbent assay. Antibodies to aMPVs were identified in five of the 15 species tested: American coots, American crows, Canada geese, cattle egrets, and rock pigeons. The presence of aMPV-specific antibodies was confirmed with virus neutralization and western blot assays. Oral swabs were collected from wild bird species with the highest percentage of aMPV-seropositive serum samples: the American coots and Canada geese. From these swabs, 17 aMPV-positive samples were identified, 11 from coots and six from geese. Sequence analysis of the matrix, attachment gene and short hydrophobic genes revealed that these viruses belong to subtype C aMPV. The detection of aMPV antibodies and the presence of virus in wild birds in Georgia, South Carolina, Arkansas and Ohio demonstrates that wild birds can serve as a reservoir of subtype C aMPV, and may provide a potential mechanism to spread aMPVs to poultry in other regions of the United States and possibly to other countries in Central and South America.

  2. Use of ovotransferrin as an antimicrobial in turkeys naturally infected with Chlamydia psittaci, avian metapneumovirus and Ornithobacterium rhinotracheale.

    Science.gov (United States)

    Van Droogenbroeck, Caroline; Dossche, Liesbeth; Wauman, Toon; Van Lent, Sarah; Phan, Thao T T; Beeckman, Delphine S A; Vanrompay, Daisy

    2011-12-15

    Respiratory pathogens are difficult to control in large-scale turkey production. This report describes a clinical trial of antimicrobial ovoTF aerosol on a large Belgian turkey farm. ovoTF was administered to reduce Chlamydia psittaci (C. psittaci) infections and to study the impact of this action on the occurrence of Ornithobacterium rhinotracheale (O. rhinotracheale) and avian metapneumovirus (aMPV) infections. Two subsequent broods were included; (i) a control brood receiving no ovoTF and (ii) an ovoTF brood receiving ovoTF aerosol (5mg/animal) at the age of 2 weeks, continuing daily for 12 days. Twenty-four one-day-old toms of the control and ovoTF brood were tagged and monitored for 15 weeks. The control brood experienced two periods of respiratory disease, the first (2-3 weeks of age) due to C. psittaci and the second (8-17 weeks of age) in the presence of C. psittaci, O. rhinotracheale and maybe aMPV. Extensive antibiotic treatment was needed in 2, 8 and 9 week-old toms. In the ovoTF brood, toms stayed healthy until the age of 9 weeks, whereafter respiratory disease occurred in the presence of C. psittaci, O rhinotracheale and aMPV. OvoTF administration: (i) reduced the amount of C. psittaci in the air as demonstrated by bioaerosol monitoring, (ii) prevented respiratory disease during the first half of the brood period, (iii) was associated with 46% reduction of mortality, and (iv) reduced the antibiotic cost. Our results justify additional clinical trials to explore the use of this innovative antimicrobial strategy for poultry. Copyright © 2011 Elsevier B.V. All rights reserved.

  3. Detection of and phylogenetic studies with avian metapneumovirus recovered from feral pigeons and wild birds in Brazil.

    Science.gov (United States)

    Felippe, Paulo Anselmo; Silva, Luciana Helena Antoniassi da; Santos, Márcia Bianchi Dos; Sakata, Sonia Tatsumi; Arns, Clarice Weis

    2011-10-01

    The aim of the present study was to determine whether avian metapneumovirus (aMPV)-related viruses were present in wild and synanthropic birds in Brazil. Therefore, we analysed samples from wild birds, feral pigeons and domestic chickens in order to perform a phylogenetic comparison. To detect the presence of aMPV, a nested reverse transcriptase-polymerase chain reaction was performed with the aim of amplifying a fragment of 270 bases for subtype A and 330 bases for subtype B, comprising the gene coding the G glycoprotein. Positive samples for aMPV subtypes A and B were found in seven (13.2%) different asymptomatic wild birds and pigeons (50%) that had been received at the Bosque dos Jequitibás Zoo Triage Center, Brazil. Also analysed were positive samples from 15 (12.9%) domestic chickens with swollen head syndrome from several regions of Brazil. The positive samples from wild birds, pigeons and domestic chickens clustered in two major phylogenetic groups: some with aMPV subtype A and others with subtype B. The similarity of the G fragment nucleotide sequence of aMPV isolated from chickens and synanthropic and wild avian species ranged from 100 to 97.5% (from 100 to 92.5% for the amino acids). Some positive aMPV samples, which were obtained from wild birds classified in the Orders Psittaciformes, Anseriformes and Craciformes, clustered with subtype A, and others from the Anas and Dendrocygma genera (Anseriformes Order) with subtype B. The understanding of the epizootiology of aMPV is very important, especially if this involves the participation of non-domestic bird species, which would add complexity to their control on farms and to implementation of vaccination programmes for aMPV.

  4. Educación en Derechos Humanos y Democracia

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    Federico Mayor Zaragoza

    2012-01-01

    Full Text Available Vivimos en un mundo de una opulencia sin precedentes, difícil de imaginar hace sesenta años. Donde no sólo se han registrado notables cambios en el terreno económico, sino en lo social y en lo político. Vivimos inmersos en la cultura de los derechos humanos. La gran idea de la humanidad ha sido y sigue siendo conciliar la universalidad de los valores con las diversas culturas. La Segunda Guerra Mundial fue uno de los episodios más atroces para la Humanidad. Por ello, y como consecuencia lógica al final de la misma, muy a pesar de la tensión que había por lo que acababa de acaecer, se procedió al diseño de un sistema de gobernación mundial basado en la igual dignidad de todos los seres humanos. Eran necesarios para evitar una nueva guerra, es decir, para construir la paz, son necesarios unos nuevos puntos de referencia, unos nuevos asideros éticos que permitan a toda ciudadanía modificar un comportamiento secular basado en la confrontación. El fomento de la dignidad no es una utopía, al contrario, es una necesidad indispensable porque sin ella no pueden concretarse los derechos y deberes humanos de los que tanto dependen la seguridad, supervivencia y felicidad de todos y todas. La pedagogía de la educación en derechos humanos es asumir que todos los seres humanos somos diferentes en nuestras características pero iguales en dignidad y derechos y, por tanto, evitar la discriminación es la finalidad principal, ya que acepta y valora la diversidad como parte de la riqueza de las relaciones humanas. Así pues, del mismo modo que en 1945 se decidió elaborar una Declaración Universal de Derechos Humanos, sería apropiado ahora, en la debacle ética que están sufriendo O ccidente y en particular la Unión Europea, una Declaración Universal sobre la Democracia, porque ha llegado el momento histórico en que la sumisión, el dominio de los pocos sobre los muchos, hemos de darlo por concluido.

  5. Los dos discursos del desarrollo humano. Las contradicciones del PNUD

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    Juan Telleria

    2014-10-01

    Full Text Available Un discurso que explícitamente afirma una idea pero implícitamente refuerza la contraria es el instrumento perfecto para desactivar cualquier iniciativa de cambio. El presente artículo muestra que éste es el caso de los Informes del Desarrollo Humano del Programa de Naciones Unidas para el Desarrollo (PNUD. Mediante un sencillo ejercicio hermenéutico se demuestra que existen fuertes contradicciones entre lo que se expresa explícitamente y lo que subyace implícitamente. El individuo supuestamente libre, clave en el concepto de desarrollo humano, deviene un autómata carente de libertad bajo el imperio de la eficiencia y la racionalidad instrumental. El PNUD, asumiendo acríticamente los fundamentos filosóficos y antropológicos que han dado forma al status quo político-cultural actual, cae en los mismos vicios que supuestamente pretende superar.

  6. A fundamentação contratualista dos direitos humanos

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    Marcelo de Araújo

    2012-05-01

    Full Text Available http://dx.doi.org/10.5007/1677-2954.2009v8n3p9 O contratualismo moral procura fundamentar a moralidade a partir do auto-interesse. No entanto, nem as teorias clássicas na tradição do contrato social, nem os representantes do contratualismo moral procuram oferecer uma fundamentação dos direitos humanos. O objetivo deste artigo é justamente apresentar uma tal fundamentação. Direitos humanos – essa é a tese central deste artigo – são tipos específicos de direitos morais de que os indivíduos podem lançar mão em sua relação com o Estado.

  7. Controlo internacional do respeito pelos direitos humanos em Portugal

    OpenAIRE

    Teles, Patrícia Galvão; Albuquerque, Catarina

    1998-01-01

    De acordo com o artigo 1°, parágrafo 3 da Carta das Nações Unidas, uma das tarefas principais desta Organização consiste na promoção e estímulo do respeito pelos direitos humanos e pelas liberdades fundamentais sem qualquer discriminação baseada na raça, sexo, língua ou religião. Desde a sua criação que a Organização das Nações Unidas (ONU) iniciou o seu trabalho de catalogação e, posteriormente, de codificação das normas de direitos humanos existentes, com vista a criar um corpo de normas vi...

  8. Aspectos sociotécnicos das TI & Relacionamento Humano & Sinergia

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    Sergio Vieira Holtz Filho

    2008-06-01

    Full Text Available As Tecnologias de Informação (TI estão entre os principais agentes de mudança sociais. Para gerir tais mudanças, uma miríade de áreas do conhecimento humano têm muito a contribuir para a abordagem dos aspectos sociotécnicos das TI e promover sinergia. Como uma organização empresarial atua no sentido de provocar mudanças no comportamento das pessoas, visando a melhoria contínua, esse processo de mudança provocada chama-se aprendizagem. Isto posto, neste artigo apresentaremos resultados observados em empresas em processo de reengenharia e submetidas a utilização de metodologias integradas (PDCA, Método Paulo Freire e Roteiro Integração de Equipes, abordando os aspectos sociotécnicos das TI & relacionamentos humanos pela praxe da pedagogia como elemento de sinergia.

  9. Europa y Estados Unidos ante los Derechos Humanos en Cuba

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    Susanne Gratius

    2008-01-01

    de la UE no han sido estrategias eficaces para promover los derechos humanos en la isla. Al ser diferentes, las políticas de la UE y EEUU incluso han contribuido a mantener el régimen castrista en el poder: la UE por su reconocimiento diplomático y la cooperación económica; EEUU, al proporcionar una imagen de enemigo externo. Por ello, y ante el nuevo panorama que ofrece el cambio de gobierno en Cuba, la próxima revisión de la Posición Común de la UE y las elecciones presidenciales en EEUU, es necesario replantearse los objetivos y estrategias políticas para incentivar los derechos humanos en Cuba.

  10. LA TUTELA SUPRACONSTITUCIONAL DE LOS DERECHOS HUMANOS EN COSTA RICA

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    Gilbert Armijo

    2003-01-01

    Full Text Available El presente artículo analiza la posición de los derechos fundamentales y la recepción de los derechos humanos en el ordenamiento jurídico de Costa Rica, considerando la eficacia de los instrumentos internacionales sobre la materia y la jurisprudencia de la Comisión y la Corte Interamericana de Derechos Humanos. El artículo analiza especialmente la jurisprudencia de la Sala Constitucional de la Corte Suprema de Costa Rica.This article analyzes the place of human rights and their reception in the Costa Rican legal system, considering the effectiveness of international treaties on the subject and the decisions of the Inter-American Commission and Court on Human Rights. The decisions of the Constitutional Chamber of the Costa Rican Supreme Court are also commented.

  11. Seguimento de um humano por um robô companheiro

    OpenAIRE

    Amorim, Diogo Samuel Bento

    2016-01-01

    Cada vez mais surgem robôs a partilhar o mesmo espaço físico que os humanos. Deste modo é importante pensar em como os robôs interagem com um humano e na partilha do mesmo espaço que eles. A forma como se movem e reagem passa a ter cada vez mais relevância e é uma componente cada vez mais presente em sistemas robóticos móveis. Aspetos relacionados com a movimentação do robô que não estão diretamente relacionados com comandos rígidos podem ter um impacto muito signi cativo na fo...

  12. Los derechos humanos hoy: perspectivas y retos XXII Conferencias Aranguren

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    Pérez Luño, Antonio-Enrique

    2014-12-01

    Full Text Available The first part, on human rights generations, analyzes the historical evolution of freedoms. Human rights were born within modernity, with marked individualistic imprint as individual freedoms that form the first phase or generation of human rights. This individualistic ideological matrix undergo an extensive process of erosion and challenge in the social struggles of the nineteenth century. These protest movements become apparent the need to complete the catalog of rights and freedoms of the first generation with a second generation of rights: economic, social and cultural rights. The protest human rights strategy is presented today with the novel unequivocally polarized around issues such as the right to peace, the rights of consumers, the right to quality of life, liberty or computer features. Based on it, it opens up, with increasing intensity, the conviction that we are dealing with a third generation of human rights complements earlier stages. The second part is the impact of teledemocracy and cyber citizenship in the human rights sphere. The theme of generations of law extends a reference to the rights of third generation nuclear aspect: The projection of the NT and ICTs in the political process of democratic societies (teledemocracy and as one of the main aspects of this phenomenon, the exercise of human rights of political participation through new technological means (cyber citizenship . We study here the main advantages and risks involved in such technology projections in the exercise of human rights.La primera parte versa sobre las generaciones de derechos humanos y analiza la evolución histórica de las libertades. Los derechos humanos nacieron en el seno de la Modernidad, con marcada impronta individualista, como libertades individuales que configuran la primera fase o generación de los derechos humanos. Dicha matriz ideológica individualista sufrirá un amplio proceso de erosión e impugnación en las luchas sociales del siglo

  13. Expresiones antagónicas en el ser humano

    OpenAIRE

    Falconí A., Fernando

    1998-01-01

    Este trabajo presenta las relaciones, la vida y la muerte, el bien y el mal, el amor y el odio/agresión, tratando de ratificar que sin la una no existe la otra pues sería totalmente invisible. Analiza estas relaciones occidente-oriente y todas las relaciones duales que se pueda generar en el ser humano Licenciado en Artes Visuales Cuenca

  14. Burocracia no Setor de Recursos Humanos de uma Autarquia

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    Luiz Fernando Almeida Pereira

    2016-11-01

    Full Text Available Recursos Humanos (RH é a área responsável por organizar e produzir os processos de gestão de pessoas dos servidores públicos de uma Autarquia. Atualmente, a burocracia que norteia os procedimentos e regras na área de RH tem sido aplicada de maneira desatualizada em algumas instituições públicas no Brasil, causando alguns problemas que por sua vez interferem no andamento eficiente, prático e objetivo dos processos nos setores de RH. Assim, o objetivo deste estudo é analisar de maneira crítica e integrativa a burocracia no setor de recursos humanos de uma Autarquia Federal Brasileira vinculada ao Ministério da Ciência, Tecnologia e Inovação (MCTI. Para isso, realiza-se um estudo de caso abordando um setor da Coordenação de Recursos Humanos desta Instituição. A tomada de decisão ou propostas de intervenção é baseada em estudo bibliográfico, confrontando com a observação da rotina do setor, incluindo os seus procedimentos executados. Após a identificação dos problemas administrativos do setor, que incluem principalmente processos desatualizados e formação acadêmica inadequada, a gestão por competências e por resultados são discutidas e propostas para orientar e ajustar os processos e fluxos de trabalho. Portanto, propõe-se neste trabalho ideias para uma flexibilidade de projetos, por meio da atualização dos fluxos de processos, que visem tornar mais eficiente o serviço público dentro do setor de recursos humanos da instituição, motivando, por conseguinte os servidores, e resultando na valorização e qualidade do trabalho do setor.

  15. Los Derechos Humanos y la Responsabilidad Social Empresarial

    Directory of Open Access Journals (Sweden)

    Alberto Carlo D´ Andreis Zapata

    2012-07-01

    Full Text Available ResumenSer responsable socialmente y respetar los Derechos Humanos ya no es un lujo, sino una necesidad, una prioridad para poder competir de una manera leal, legal y sostenible, toda vez que el Pacto Mundial de la Naciones Unidas ha considerado que el respeto o aplicación de los Derechos Humanos debe ser una prioridad para las empresas y no puede seguir tratándose como una acción propia de las acciones de gobiernos, sino que también hace parte de las acciones de las empresas, quienes tienen obligaciones sociales y legales con todos los grupos de interés, por eso en este artículo de investigación hemos decidido resaltar nuevas cuestiones relacionadas con los derechos humanos, así como identificar y promocionar herramientas y recursos de utilidad en el campo de los derechos humanos que como producto de nuestra investigación consideramos que se traducen en buenas prácticas para las empresas.AbstractBeing socially responsible and respect human rights is no longer a luxury but a necessity, a priority in order to compete in a fair, legal and sustainable, since the Global Compact United Nations considered that respect or application of Human rights should be a priority for businesses and can not longer be treat- ed as a custom action in the actions of governments, but also part of the shares of the companies who have social and legal obligations to all stakeholders, so in this article we have chosen to highlight new research issues related to human rights and to identify and promote useful tools and resources in the field of human rights as a result of our research we consider that translate into best practices for companies.

  16. El Proyecto Genoma Humano: aspectos éticos

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    Hernández Yago, José

    1999-02-01

    Full Text Available Not available.Todo conocimiento científico es positivo en cuanto que su contenido incrementa el control de la realidad. El desciframiento del genoma humano, con la consiguiente identificación y localización de los 50.000 a 100.000 genes que configuran su patrimonio genético, permitirá el progresivo esclarecimiento de los mecanismos reguladores de la expresión génica y pondrá al alcance del hombre posibilidades espléndidas, que requerirá años desentrañar. El acelerado ritmo en la caracterización de nuevos genes está desencadenando la posibilidad de pronosticar un número cada vez mayor de enfermedades genéticas y, por tanto, puede propiciar la práctica de una Medicina preventiva cada vez más rigurosa. Sin embargo, las posibles aplicaciones del conocimiento científico que aporta este proyecto suscitan también importantes inquietudes, desde el momento en que cada ser humano puede convertirse en un ser sin intimidad biológica. Por otra parte, la tecnología del ADN recombinante, al tiempo que aporta la enorme posibilidad de una futura terapia génica, suscita también legítimas preocupaciones sobre el correcto uso de estos conocimientos. En este artículo se abordan algunas de las cuestiones de carácter ético que ya plantea actualmente el Proyecto del Genoma Humano. Se adopta la perspectiva según la cual el respeto al patrimonio genético debería estar siempre presidido por un principio básico: no perder nunca de vista al hombre, esto es, al ser humano y su derecho a la identidad personal propia.

  17. Desenvolvimento humano: contribuições da psicologia moral

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    Yves de La Taille

    2007-03-01

    Full Text Available Procuramos avaliar as contribuições do conhecimento acumulado pela Psicologia Moral para a compreensão do desenvolvimento humano, para sustentar a tese segundo a qual a fonte energética do dever moral precisa ser procurada não só em sentimentos exclusivamente morais, mas também em sentimentos que desempenham um papel para o próprio desenvolvimento humano no seu conjunto. Três são os passos da análise deste artigo. Em primeiro lugar, verifi car se há possibilidade de articulação entre teorias psicológicas que enfatizam a dimensão afetiva da moralidade (Freud e Durkheim e outras que enfatizam a dimensão intelectual (Piaget e Kohlberg. Em segundo lugar, uma vez constatada a impossibilidade dessa articulação, sustentar que o sentimento de vergonha, presente na moralidade mas também em outras dimensões do desenvolvimento humano, é condição necessária ao sentimento de obrigatoriedade. Finalmente, analisar se tal sentimento é passível de ser evocado como fonte energética essencial nas abordagens que enfatizam a dimensão intelectual da moralidade.

  18. O contrário dos direitos humanos (explicitando Zizek

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    J. A. Lindgren Alves

    2002-06-01

    Full Text Available O presente artigo analisa a situação atual dos direitos humanos, guiado pelas afirmações críticas do autor esloveno Slavoj Zizek, quando defende que a nova normatividade emergente para os direitos humanos acaba por ser a forma em que aparece o seu exato oposto. Abarcando os atentados de 11 de setembro, sem que isso se constitua o foco central do estudo, o artigo avalia a validade dessa afirmação ante as vicissitudes empíricas com que os direitos humanos vêm se defrontando nos últimos anos.The article analyses the current situation of human rights, guided by the critic affirmations of the Slovene author Slavoj Zizek, when he supports that the new emerging normativity of the human rights ends to be the form of appearance of its very opposite. Contemplating the 11th of September, without this constitutes the central focus of the study, the article evaluates the validity of these affirmations the empirical vicissitudes which the human rights have been facing in the last years.

  19. Los Derechos Humanos en América Latina

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    Jovanny Boss Agudelo

    2012-07-01

    Full Text Available ResumenEl objeto central del presente artículo de reflexión se sitúa en la promoción y protección de los Derechos Humanos en América Latina. Para su desarrollo, se parte de una aproximación conceptual a la idea de los Derechos Humanos y luego se da paso a un análisis del estado de éstos (inclusión, conquista y evolución en el territorio en el contexto iberoamericano. La finalidad es responder a la pregunta por la vigencia y legitimidad de los Derechos Humanos en el marco de los grandes conflictos que han determinado a la región a lo largo del siglo XX, con especial énfasis a partir de 1948, año en que se promulga la Declaración Universal de los Derechos Humanos y nace el Derecho Internacional Humanitario como una aspiración civilista de occidente, con proyección a la defensa de la familia humana sin discriminación de nacionalidad u origen cultural. El ensayo finaliza elevando una propuesta para la intervención civil en el respeto y protección de los Derechos Humanos, desde la condición de ciudadanía como paso fundamental a la condición civil de la persona ante el Estado. AbstractThe central object of this paper is placed on the promotion and protection of human rights in Latin America. For its development of a conceptual approach to the idea of human rights and then leads a discussion of the status of these (including, conquest and evolution in the area in the Latin American context. The purpose is to answer the question of the validity and legitimacy of human rights in the context of the major conflicts that have shaped the region over the twentieth century, with special emphasis since 1948, promulgating the Declaration universal human Rights and International Humanitarian Law is born, as a civilian aspiration of the West, with projection to the defense of the human family without distinction of nationality or cultural background. The essay ends with a proposal for bringing civil action in respect and protection

  20. Derechos de los animales, deberes de los humanos

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    López de la Vieja, María Teresa

    2005-06-01

    Full Text Available Applied Ethics usually refers to human beings, to their practical problems and to their interests. However, the sphere of ethical issues has expanded during the last years. In spite of the fact that the moral point of view is still grounded on anthropocentrism, the environment and living beings are now the aim object of some moral concern. Justice, as a moral principle, could expand itself, as well, in order to include nonhuman beings: their interest and their welfare. Prom a moderate anthropocentric standpoint, this article defends an asymmetrical moral concern. Indeed, we could understand animal' s rights only as a result of human duties towards the own species. This essay claims for direct duties to future generations of humans, and for indirect duties to nonhUman. Therefore, justice between species would enlarge justice between generations.

    La Ética aplicada se suele referir a los problemas prácticos e intereses de los seres humanos. Sin embargo, las cuestiones éticas se han ido ampliando en los últimos años. A pesar de que el punto de vista moral sigue anclado en un enfoque antropocéntrico, el medio ambiente y los seres vivos son ahora objeto de alguna consideración moral. La justicia, como principio moral, puede ampliarse también hasta incluir a los no humanos, sus intereses y su bienestar. Desde un enfoque antropocéntrico moderado, el artículo defiende en este tema una consideración moral asimétrica; pues sólo podemos entender los derechos de los animales como resultado de los deberes de los humanos hacia la propia especie. Son deberes directos hacia las generaciones futuras de humanos, deberes indirectos hacia los no humanos. Por tanto, la justicia entre especies amplía la idea de justicia entre generaciones.

  1. Methyltransferase-defective avian metapneumovirus vaccines provide complete protection against challenge with the homologous Colorado strain and the heterologous Minnesota strain.

    Science.gov (United States)

    Sun, Jing; Wei, Yongwei; Rauf, Abdul; Zhang, Yu; Ma, Yuanmei; Zhang, Xiaodong; Shilo, Konstantin; Yu, Qingzhong; Saif, Y M; Lu, Xingmeng; Yu, Lian; Li, Jianrong

    2014-11-01

    Avian metapneumovirus (aMPV), also known as avian pneumovirus or turkey rhinotracheitis virus, is the causative agent of turkey rhinotracheitis and is associated with swollen head syndrome in chickens. Since its discovery in the 1970s, aMPV has been recognized as an economically important pathogen in the poultry industry worldwide. The conserved region VI (CR VI) of the large (L) polymerase proteins of paramyxoviruses catalyzes methyltransferase (MTase) activities that typically methylate viral mRNAs at guanine N-7 (G-N-7) and ribose 2'-O positions. In this study, we generated a panel of recombinant aMPV (raMPV) Colorado strains carrying mutations in the S-adenosyl methionine (SAM) binding site in the CR VI of L protein. These recombinant viruses were specifically defective in ribose 2'-O, but not G-N-7 methylation and were genetically stable and highly attenuated in cell culture and viral replication in the upper and lower respiratory tracts of specific-pathogen-free (SPF) young turkeys. Importantly, turkeys vaccinated with these MTase-defective raMPVs triggered a high level of neutralizing antibody and were completely protected from challenge with homologous aMPV Colorado strain and heterologous aMPV Minnesota strain. Collectively, our results indicate (i) that aMPV lacking 2'-O methylation is highly attenuated in vitro and in vivo and (ii) that inhibition of mRNA cap MTase can serve as a novel target to rationally design live attenuated vaccines for aMPV and perhaps other paramyxoviruses. Paramyxoviruses include many economically and agriculturally important viruses such as avian metapneumovirus (aMPV), and Newcastle disease virus (NDV), human pathogens such as human respiratory syncytial virus, human metapneumovirus, human parainfluenza virus type 3, and measles virus, and highly lethal emerging pathogens such as Nipah virus and Hendra virus. For many of them, there is no effective vaccine or antiviral drug. These viruses share common strategies for viral gene

  2. Local and systemic immune responses following infection of broiler-type chickens with avian Metapneumovirus subtypes A and B.

    Science.gov (United States)

    Rautenschlein, Silke; Aung, Ye Htut; Haase, Christine

    2011-03-15

    Infections with avian Metapneumovirus (aMPV) are often associated with swollen head syndrome in meat type chickens. Previous studies in turkeys have demonstrated that local humoral and cell-mediated immunity plays a role in aMPV-infection. Previous experimental and field observations indicated that the susceptibility of broilers and their immune reactions to aMPV may differ from turkeys. In the presented study local and systemic immune reactions of broilers were investigated after experimental infections with subtypes A and B aMPV of turkey origin. Both virus subtypes induced a mild respiratory disease. The recovery from respiratory signs correlated with the induction of local and systemic aMPV virus-neutralizing antibodies, which began to rise at 6 days post infection (dpi), when the peak of clinical signs was observed. In a different manner to the virus neutralizing (VN) and IgG-ELISA serum antibody titres, which showed high levels until the end of the experiments between 24 and 28 dpi, the specific IgA-ELISA and VN-antibody levels in tracheal washes decreased by 10 and 14 dpi, respectively, which may explain the recurring aMPV-infections in the field. Ex vivo cultured spleen cells from aMPV-infected broilers released at 3 and 6 dpi higher levels of IFN-γ after stimulation with Concanavalin A as compared to virus-free birds. In agreement with studies in turkeys, aMPV-infected broilers showed a clear CD4+ T cell accumulation in the Harderian gland (HG) at 6 dpi (P<0.05). In contrast to other investigations in turkeys aMPV-infected broilers showed an increase in the number of CD8alpha+ cells at 6 dpi compared to virus-free birds (P<0.05). The numbers of local B cells in the Harderian gland were not affected by the infection. Both aMPV A and B induced up-regulation of interferon (IFN)-γ mRNA-expression in the nasal turbinates, while in the Harderian gland only aMPV-A induced enhanced IFN-γ expression at 3 dpi. The differences in systemic and local T cell and

  3. Incidence and Risk Factors for Respiratory Syncytial Virus and Human Metapneumovirus Infections among Children in the Remote Highlands of Peru.

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    Andrew Wu

    Full Text Available The disease burden and risk factors for respiratory syncytial virus (RSV and human metapneumovirus (MPV infections among children living in remote, rural areas remain unclear.We conducted a prospective, household-based cohort study of children aged <3 years living in remote rural highland communities in San Marcos, Cajamarca, Peru. Acute respiratory illnesses (ARI, including lower respiratory tract infection (LRTI, were monitored through weekly household visits from March 2009 through September 2011. Nasal swabs collected during ARI/LRTI were tested for RSV, MPV, and other respiratory viruses using real-time RT-PCR. Incidence rates and rate ratios were calculated using mixed effects Poisson regression.Among 892 enrolled children, incidence rates of RSV and MPV ARI were 30 and 17 episodes per 100 child-years, respectively. The proportions of RSV and MPV ARI that presented as LRTI were 12.5% and 8.9%, respectively. Clinic visits for ARI and hospitalizations were significantly more frequent (all p values <0.05 among children with RSV (clinic 41% and hospital 5.3% and MPV ARI (38% and 3.5% when compared with other viral infections (23% and 0.7% and infections without virus detected (24% and 0.6%. In multivariable analysis, risk factors for RSV detection included younger age (RR 1.02, 95% CI: 1.00-1.03, the presence of a smoker in the house (RR 1.63, 95% CI: 1.12-2.38, residing at higher altitudes (RR 1.93, 95% CI: 1.25-3.00 for 2nd compared to 1st quartile residents; RR 1.98, 95% CI: 1.26-3.13 for 3rd compared to 1st quartile residents. Having an unemployed household head was significantly associated with MPV risk (RR 2.11, 95% CI: 1.12-4.01.In rural high altitude communities in Peru, childhood ARI due to RSV or MPV were common and associated with higher morbidity than ARI due to other viruses or with no viral detections. The risk factors identified in this study may be considered for interventional studies to control infections by these viruses

  4. Regresiones en materia de derechos humanos en España: una perspectiva europea

    OpenAIRE

    Guillén Lanzarote, Aida; Saura Estapà, Jaume

    2013-01-01

    La reciente sentencia del Tribunal Europeo de Derechos Humanos en el caso de Inés del Río ha oscurecido mediáticamente el no menos interesante informe del Comisario para los Derechos Humanos del Consejo de Europa, de 9 de octubre de 2013, que pone en entredicho el comportamiento de los sucesivos gobiernos españoles ante la crisis con base a los estándares básicos de derechos humanos.

  5. El derecho humano a la fecundación in vitro

    OpenAIRE

    Zegers Hochschild,Fernando; Dickens,Bernard M; Dughman Manzur,Sandra

    2014-01-01

    La Corte Interamericana de Derechos Humanos dictaminó que la sentencia de la Corte Suprema de Justicia de Costa Rica del año 2000, que prohibió la fecundación in vitro (FIV), infringe el derecho humano a la vida privada y la vida familiar, el derecho humano a fundar y criar una familia, y el derecho humano a la no discriminación en base a discapacidad, situación económica, o género. Las conclusiones de la Corte Interamericana respecto de las violaciones a la Convención Americana de Derechos H...

  6. DIREITO AMBIENTAL E HUMANO: A complexidade na questão da água

    OpenAIRE

    Grubba, Leilane Serratine

    2013-01-01

    Este artigo tem por objeto os direitos humanos dentro do contexto da complexidadeambiental. Nesse sentido, aborda os recursos naturais para a satisfaçãodigna da vida humana e tem por objetivo problematizar a questãoda água no contexto da sociedade contemporânea e para a concretizaçãodos direitos humanos. Assim, os direitos humanos são percebidos a partirda metodologia da complexidade, quer dizer, o conhecimento não pode serreducionista da realidade, a exemplo da idealidade dos direitos humano...

  7. Investimento no capital humano da AP, factor essencial na resolução da crise

    OpenAIRE

    Rato, Helena; Silva, Matilde Gago da

    2011-01-01

    Becker e outros cientistas das ciências humanas levaram ao desenvolvimento da teoria do capital humano. O desenvolvimento da sociedade do conhecimento e as TIC reforçaram o reconhecimento da importância do capital humano. Contudo, os países da sociedade do conhecimento enfrentam uma ameaça de retrocesso, devido ao desajuste dos indicadores do desempenho macroeconómico e à dificuldade de mensuração do investimento em capital humano. A resolução da crise actual exige que o capital humano da Adm...

  8. Una mirada al Sistema Interamericano de Derechos Humanos

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    Ariel E. DULITZKY

    2009-11-01

    Full Text Available RESUMEN: El artículo analiza el debate actual que se da en el ámbito del sistema interamericano de derechos humanos. En primer lugar examina la evolución de la situación en América Latina en los últimos 20 años, mostrando como el paso de dictaduras a gobiernos elegidos democráticamente no necesariamente se reflejó en una mejoría en los derechos humanos. Luego de describir el funcionamiento de la Comisión y la Corte Interamericanas de Derechos Humanos, analiza dos tendencias presentes en el actual debate sobre el futuro del sistema interamericano. Una posición calificada como "promoción-reforma" entiende que el sistema debería orientarse hacia actividades exclusivamente de promoción de los derechos humanos antes que tareas de protección. Para ello proponen reformar la Convención Americana sobre Derechos Humanos. La segunda posición , la denomina "protección-perfeccionamiento" ya que sostiene que la realidad actual de América Latina, requiere que la Comisión y la Corte se dediquen fundamentalmente a la protección de los derechos humanos. Finalmente el artículo presenta cinco objetivos que deberían orientar el trabajo del sistema y muestra cómo las dos posiciones anteriormente mencionadas difieren en cuanto a estos objetivos o a la manera de alcanzarlos.ABSTRACT: The article analizes the current debate within the inter-American System of human rights. The article begins by describing the evolution of the human rights situation in Latin-America over the last 20 years, from military dictatorships to elected goverments. The article points out that these changes do not necessarily imply an improvement in the enjoyment of human rights. After describing the functioining of the Inter-American Commission and Court, the article .describes two different lines of thought in the present debate regarding the future of the Inter-American System. The first position "promotion-reform" suggests that the inter-American system should deal

  9. Estudo do efeito da interferencia por RNA (RNAi) na replicação do metapneumovirus aviario (AMPV) subtipo A in vitro

    OpenAIRE

    Helena Lage Ferreira

    2007-01-01

    Resumo: O metapneumovírus aviário (AMPV) é o agente primário da rinotraqueíte dos perus (TRT). O AMPV pertence à família Paramyxoviridae, subfamília Pneumovirinae, gênero Metapneumovirus. Também está associado à síndrome da cabeça inchada (SHS) em galinhas e é responsável por significativas perdas econômicas em sua produção. O presente estudo foi dividido em três partes. A primeira parte do trabalho consistiu em avaliar a beta-actina, gene utilizado como controle interno das técnicas molecula...

  10. El capital humano, otro activo de su empresa

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    Marino Valencia Rodríguez

    2005-01-01

    Full Text Available En un ambiente como el actual, de gran competencia, cambios rápidos y permanentes, la supervivencia de una organización depende de su capacidad para generar valor agregado a sus clientes y proveedores, lo cual será la base de su competitividad y posicionamiento en el mercado, pero esto, a su vez, depende de la capacidad de innovación, velocidad de respuesta, adaptabilidad al cambio y habilidad para detectar las necesidades de los participantes en la cadena de valor de la organización. El capital humano constituye un activo intangible que tiene la capacidad de apoyar el incremento de la productividad, impulsar la innovación y con ello la competitividad. Su misma naturaleza le da características definitivamente distintivas de cualquier otro tipo de recurso. Sin lugar a dudas el éxito de cualquier organización se vincula estrechamente al desempeño de todas las personas que la integran, sin importar su jerarquía, ubicación y condición, pues son ellas las que poseen habilidades, conocimientos, relaciones sociales, actitudes, valores, etc., y son ellas las que deciden dónde, cuándo y cómo utilizarlos. Los recursos humanos son fuente de ventaja competitiva de las organizaciones, por ello las empresas cada vez en mayor medida deben identificar el valor que la persona tiene para la organización y tratar de potenciarlo, al igual deben incrementar el valor que la empresa tiene para el empleado, con el fin de captar a las mejores personas y reducir el riesgo de transferibilidad de un activo tan valioso como es el capital humano.

  11. Estudio de Recursos Humanos del Sector Salud en Colombia.

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    Gustavo Malagón Londoño

    1997-12-01

    Full Text Available

    Antecedentes


    Siendo el recurso humano el en cargado de movilizar los demás recursos e impulsarlos de tal manera que conlleve a una dinámica de producción masiva de bienes o servicios, su estudio para llegar a una identificación real, categorización, clasificación; es necesario en cualquier área del conocimiento.

    Frente al nuevo esquema de prestación de servicios propuestos, los profesionales de la salud han perdido aquella posición digna, de respeto y liderazgo dentro del sistema y dentro de su entorno social, lo cual ha llevado a muchos de ellos a desertar de sus oficios originales, con lo cual se llega a la falla en la instauración del Sistema.

    Así mismo la Ley 100 de 1993 que debió ser vista como una nueva oportunidad de negocios dentro de las diferentes profesiones de la salud, se transformó en una seria amenaza para el ejercicio independiente de cada profesión.

    No es apropiado ni satisfactorio dejar un proceso decisorio de tal magnitud en manos de otros sectores que únicamente verán y obtendrán estadísticas del acceso de la población colombiana a estudios superiores y los renglones en los cuales quieren desempeñarse y se desempeñarán en un futuro. El sector de la salud debe tomar un claro papel protagónico frente a la promulgación de políticas de formación y utilización de su recurso humano.

    La Academia Nacional de Medicina consciente del vacío existente en el sector con respecto al tema, ha querido desde su posición estratégica con respecto a las profesiones del área y las universidades, liderar un proceso que conlleve a la organización de un Estudio del recurso humano del Sector Salud en Colombia, tomando como punto de partida el censo de los trabajadores de la salud en todas las áreas, de tal manera que se identifique su disponibilidad, su capacidad, la ubicación, las facilidades laborales en zonas aisladas y su grado de satisfacción, entre otras

  12. Derechos humanos y el derecho penal en el ciberespacio

    Directory of Open Access Journals (Sweden)

    Pierre Gilles Bélanger

    2017-11-01

    Full Text Available En la medida en que el tráfico de estupefacientes, el fraude, el blanqueo de dinero y toda una gama de otros delitos trascienden las fronteras nacionales, los responsables de luchar contra esta delincuencia deben encontrar medios eficaces para obtener las pruebas necesarias que, naturalmente, se encontrarán a menudo más allá de sus propias fronteras. Hace ya casi quince años se implementó un instrumento que facilita ese acceso. El Convenio sobre la Ciberdelincuencia, conocido también como Convenio de Budapest sobre la Ciberdelincuencia o Convenio de Budapest, es el primer tratado internacional que intenta abordar los delitos informáticos y los delitos en Internet, incluida la pornografía infantil, armonizando ciertas leyes nacionales, mejorando las técnicas de investigación y aumentando la cooperación entre las naciones y la adecuada protección de los derechos humanos y las libertades en cumplimiento del Convenio Europeo para la Protección de los Derechos Humanos y de las Libertades Fundamentales. La realidad de 2017 es que la presencia de nuevos elementos, especialmente relativos a la recopilación de datos más personales en el ciberespacio, plantea nuevos desafíos y requiere nuevas ideas. El Convenio permite esta ampliación mediante reflexiones, directrices nuevas y protocolos. Está abierto a todos y es el único instrumento de este tipo. No está completo, sino que deja lugar a la adopción de protocolos futuros y, sobre todo, permite a los grupos de trabajo, entre los regionales que trabajan en el campo de la ciberdelincuencia o la asistencia judicial recíproca, desarrollar nuevas maneras de fortalecer la lucha contra la delincuencia, respetando al mismo tiempo los derechos humanos.

  13. Humanismo y poshumanismo: dos visiones del futuro humano

    Directory of Open Access Journals (Sweden)

    Mariana Isabel Herazo-Bustos

    2015-01-01

    Full Text Available El humanismo y el poshumanismo son movimientos intelectuales diferentes y relacionados para el entendimiento del ser humano. El humanismo renacentista y de la modernidad plantea que el ser humano es el sujeto autónomo de la modernidad, dueño de la voluntad y poseedor único de la razón, que lo distingue de las demás especies. Este humanismo, vi - sión del mundo que sustentó el pensamiento occidental durante años, ha sido severamente criticado por los filósofos que destacaron sus limitaciones teóricas y éticas inspiradas por la aparición de la cibernética y las nuevas tecnologías. El poshumanismo, mediante la investigación científica y las tecnologías avanzadas, aboga por la reconfiguración de la naturaleza humana y la creación de seres biológica y tecnológicamente superiores al homo sapiens; esto es posible mejorando el funcionamiento del cerebro, controlando la procreación, retrasando el envejecimiento, y así lograr la inmortalidad. El acaecimiento de esta forma de ver mundo puede ser superado desde la nostalgia humanística, exaltando en su totalidad las cualidades propias de la naturaleza humana y dándole sentido racional y moral a la vida. El proyecto de mejoramiento humano ejerce influencia amplia y profunda sobre la cultura y la sociedad contemporánea, por ello se ofrece una crítica desde un acercamiento sinérgico de estas posturas

  14. Lazer como pratica educativa : as possibilidades para o desenvolvimento humano

    OpenAIRE

    Maira Ivone Lombardi

    2005-01-01

    Resumo: Os objetivos principais deste trabalho consistem em conhecer as inter-relações existentes entre lazer, educação, animação sociocultural e desenvolvimento humano integral e quais as possibilidades de se contribuir para a emancipação do indivíduo na sociedade, utilizando o lazer como prática educativa. Já os objetivos mais específicos são fundamentais à proposição de que o lazer como prática educativa, com base nos seis conteúdos culturais do lazer, pode contribuir para a formação de um...

  15. Interacción entre humanos y animales

    OpenAIRE

    von Arcken Cancino, Berta Constanza

    2011-01-01

    En este artículo se presenta una breve reflexión, apoyada en revisión documental, sobre el contenido ético presente en los estudios y análisis que se realizan alrededor de la interacción entre humanos y animales. Se inicia con el tema de la domesticación animal, actividad pionera de las relaciones de conquista y dominio del hombre sobre la naturaleza; se pasa a los diferentes usos que se hacen de los animales, desde la Antigüedad hasta el presente, resaltando la relación con los animales de c...

  16. Emprendimiento: visiones desde las teorias del comportamiento humano

    OpenAIRE

    Montoya, Jorge Andrés Marulanda; Calle, Geovanny Correa; Mejía, Luis Fernando Mejía

    2013-01-01

    El artículo tiene como objeto presentar una aproximación al emprendimiento desde el marco teórico del comportamiento humano, visto desde la perspectiva de las teorías Etológicas y principalmente desde el punto de vista psicológico. Se busca mediante el análisis identificar las características internas y las habilidades (competencias) que poseen los emprendedores, respondiendo así a la pregunta de si los emprendedores se hacen o nacen.

  17. Perfeccionamiento de los recursos humanos en archivología

    Directory of Open Access Journals (Sweden)

    Teresa Fittipaldi Huelmo

    2013-11-01

    Full Text Available Se analiza la situación y las posibles soluciones para mejorar los recursos humanos disponibles en el área de Archivología. Este tema está considerado en varios aspectos: la formación docente de docentes e investigadores; la calidad y la cantidad de los estudiantes; la capacitación de las personas que se encuentran realizando tareas en Archivos. El tema se analiza en el marco de la situación concreta del Uruguay. 

  18. Microquimerismo natural ¿Existen humanos con varios genomas?

    OpenAIRE

    José Luis Quirós Alpízar; Isabel Cristina Arce Jiménez

    2010-01-01

    El microquimerismo humano se define como la presencia dentro de un individuo de una pequeña población de células originadas en un individuo genéticamente distinto. El microquimerismo adquirido de forma natural se desarrolla a lo largo del embarazo, como una consecuencia del ampliamente demostrado tráfico bidireccional transplacentario de células. Esta trasferencia resulta en un estado microquimérico a décadas posparto, tanto para la madre (microquimerismo fetal) como para su progenie (microqu...

  19. Aproximación al concepto de los derechos humanos

    OpenAIRE

    Velasco Arroyo, Juan Carlos

    1990-01-01

    Los derechos humanos han alcanzado un amplísimo reconocimiento jurídico en cuanto base normativa tanto de los actuales sistemas democráticos como de los múltiples organismos que articulan unas relaciones internacionales cada vez más intensas. En dicha noción normativa no se incluyen, sin embargo, tan sólo aspectos jurídico-formales. En su concepto se dan cita diversos atributos formales (universalidad de su adscripción, carácter absoluto e inalienabilidad, entre otras notas ...

  20. Tratamiento del agua para consumo humano en el hogar

    OpenAIRE

    Instituto Nacional de Salud

    2008-01-01

    El agua es esencial para la vida y la accesibilidad del ser humano a ella le ha permitido asentarse, desarrollarse, recrearse y asegurar su supervivencia y salud. El uso cotidiano donde hombres, mujeres, niños y ancianos hacen uso de ésta, se relaciona con el acceso del agua para satisfacer necesidades básicas como el agua para bebida, higiene personal y alimentación. La privación del acceso del agua dulce ya sea en cantidades o calidades adecuadas pone en riesgo su salud y calidad de vida...

  1. Sobrepoblación penitenciaria y derechos humanos

    OpenAIRE

    Salinas, Raúl

    2017-01-01

    De acuerdo con los tratados internacionales de Derechos Humanos y las disposiciones constitucionales la aplicación de violencia estatal a modo de castigo sólo es legítima en la medida en que resulte respetuosa de los derechos individuales. La realidad indica que las violaciones a los derechos fundamentales en las cárceles son múltiples e intensas. El presente trabajo es el desarrollo jurídico sobre la sobrepoblación penitenciaria y las políticas públicas que la anteceden y posibilitan. La ...

  2. Emprendimiento: visiones desde las teorias del comportamiento humano

    Directory of Open Access Journals (Sweden)

    Jorge Andrés Marulanda Montoya

    2009-04-01

    Full Text Available El artículo tiene como objeto presentar una aproximación al emprendimiento desde el marco teórico del comportamiento humano, visto desde la perspectiva de las teorías Etológicas y principalmente desde el punto de vista psicológico. Se busca mediante el análisis identificar las características internas y las habilidades (competencias que poseen los emprendedores, respondiendo así a la pregunta de si los emprendedores se hacen o nacen.

  3. Direitos humanos e globalização

    OpenAIRE

    Almeida, Fábio

    2010-01-01

    Repensar, hoje, os direitos humanos, significa reflectir acerca das relações a estabelecer, no que concerne à sua análise e implementação, entre os direitos civis e políticos, por um lado, e os direitos económicos, sociais e culturais, por outro. O neoliberalismo sempre afirmou a prevalência ou sobredeterminação dos primeiros sobre os segundos, enquanto as correntes do socialismo democrático ou de concepções liberais mais igualitárias têm advogado uma profunda imbricação dos segundos com os p...

  4. SEGURIDAD EN ACTIVOS DE INFORMACIÓN HUMANOS

    OpenAIRE

    Jorge Mario Cadavid-Aguirre

    2013-01-01

    Las empresas invierten grandes cantidades de dinero en estrategias de seguridad orientadas al control tecnológico: Antivirus, FireWall, Cifrado, IPS, IDS , pero a pesar de todos esos esfuerzos siguen siendo vulneradas. El problema radica en que dichas tecnologías no cubren el activo que más interactúa con la información; El ser humano. Está ponencia tuvo como objetivo concientizar al usuario final, hacerle ver la importancia de sus hábitos y comportamientos para mantener niveles seguros en su...

  5. Universalismo, relativismo e direitos humanos: uma revisita contingente

    OpenAIRE

    Benvenuto,Jayme

    2015-01-01

    Este artigo revisita o debate em torno do universalismo e do relativismo cultural aplicado aos direitos humanos, buscando atualizá-lo a partir de situações verificadas nas últimas décadas em certas partes do mundo, incluindo o Brasil. Assume a defesa do relativismo contingente sob a influência do filósofo norte-americano Richard Rorty. Conclui com a aplicação da perspectiva da educação sentimental, desenvolvida pelo filósofo citado, ...

  6. Universalismo, relativismo e direitos humanos: uma revisita contingente

    OpenAIRE

    Benvenuto, Jayme

    2015-01-01

    Este artigo revisita o debate em torno do universalismo e do relativismo cultural aplicado aos direitos humanos, buscando atualizá-lo a partir de situações verificadas nas últimas décadas em certas partes do mundo, incluindo o Brasil. Assume a defesa do relativismo contingente sob a influência do filósofo norte-americano Richard Rorty. Conclui com a aplicação da perspectiva da educação sentimental, desenvolvida pelo filósofo citado, a recentes declarações dos parlamentares brasileiros Jair Bo...

  7. Los derechos humanos laborales en el Derecho Internacional

    OpenAIRE

    Canessa, Miguel

    2009-01-01

    El presente estudio pretende abordar esa temática desde el horizonte del Derecho internacional. En el primer acápite se plantea un concepto para este selecto grupo de derechos laborales básicos, así como se postulan los argumentos que justifican su reconocimiento jurídico en los instrumentos internacionales de derechos humanos. En el segundo acápite se aborda la identificación de los derechos laborales con los derechos sociales, como una forma de desvalorizar su relevancia jurídica. En el ter...

  8. As alavancas do corpo humano jogando com a interdisciplinaridade

    OpenAIRE

    Andrade, Fábio Luiz [UNESP

    2015-01-01

    Essa dissertação evidenciou conceitos da Física – Alavancas, que se inter-relacionam com os da Biologia, os quais explicam o funcionamento de algumas funções do corpo humano. Estes foram abordados de forma interdisciplinar, por meio de aulas teóricas e práticas, com atividades de experimentação, para que dessa forma os alunos possam assimilar melhor os conceitos complexos que as alavancas proporcionam e saibam identificá-los no dia a dia. Para tanto é fundamental que o aluno compreenda os pri...

  9. Os direitos humanos e a responsabilidade do credor internacional

    Directory of Open Access Journals (Sweden)

    Douglas Antônio Rocha Pinheiro

    2014-09-01

    Full Text Available Partindo do conceito moderno de dívida odiosa e da política creditícia do Banco Mundial, o presente artigo pretende destacar a viabilidade de se exigir o respeito aos direitos humanos como requisito indispensável ao empréstimo internacional. Based on the modern concept of odious debt and the loan policy of the World Bank, this article intends to point out the viability of requiring respect for human rights as an essential condition for the international lending.

  10. Pedagogia crítica e Direitos Humanos: fundamentos para uma proposta pedagógico-crítica em Direitos Humanos

    OpenAIRE

    Carvalho, Maria Elizete Guimarães; Estêvão, Carlos Alberto Vilar

    2013-01-01

    Este artigo reflete sobre a necessidade de encontrar uma teoria pedagógica que ofereça sustentação à ação educativa em Direitos Humanos, apresentando uma proposta pedagógico-crítica alicerçada nos pressupostos da teoria e da pedagogia críticas, privilegiando a pedagogia freireana, numa perspectiva atualizada para os Direitos Humanos. Nesse sentido, o trabalho tem o propósito de discutir um fundamento teórico-crítico para a Educação em Direitos Humanos, buscando novas formas de investigação e ...

  11. The effect of reduced treatment time and dosage of enrofloxacin on the course of respiratory disease caused by avian metapneumovirus and Ornithobacterium rhinotracheale.

    Science.gov (United States)

    Garmyn, A; Martel, A; Froyman, R; Ludwig, C; Nauwynck, H; Haesebrouck, F; Pasmans, F

    2009-11-01

    A dose titration and reduced duration medication study were performed to evaluate the current enrofloxacin treatment schedule in growing turkeys experimentally infected with avian metapneumovirus and Ornithobacterium rhinotracheale. Experimental groups of 17 four-week-old turkeys were first infected with avian metapneumovirus and 3 d later with O. rhinotracheale. Enrofloxacin treatment in the drinking water was started 24 h after O. rhinotracheale inoculation. In the dose titration study, enrofloxacin doses of 5, 10, and 20 mg/kg of BW were administered for 5 successive days. In the reduced duration medication study, the following enrofloxacin regimens were compared: 25 mg/kg of BW per day on d 0 and 2; 15 mg/kg of BW per day on d 0, 2, and 4; and 10 mg/kg of BW for 5 successive days. In both studies, all enrofloxacin treatments were equally efficacious (i.e., equally capable of shortening the course of clinical disease), eliminating O. rhinotracheale from the respiratory tract and reducing gross lesions. Ornithobacterium rhinotracheale bacteria were not recovered from any of the birds on enrofloxacin-supplemented media, indicating that none of the used treatment regimens promoted the selection of bacterial clones with reduced susceptibility or resistance to this antimicrobial agent. In conclusion, none of the alternative enrofloxacin treatment regimens yielded better results than the current prescribed treatment (i.e., 10 mg/kg of BW for 5 successive days) of O. rhinotracheale infections in turkeys. However, the reduced duration of application would offer a less time-consuming and equally effective alternative.

  12. Capital humano y capacidad humana Human capital and human capacity

    Directory of Open Access Journals (Sweden)

    Sen Amartya Kumar

    1998-12-01

    Full Text Available En este articulo se examinan las relaciones y diferencias entre el concepto de 'capital humano' y el concepto de 'capacidad humana'. El concepto de capital humano es mas limitado puesto que solo concibe las cualidades humanas en su relación con el crecimiento económico mientras que el concepto de capacidades da énfasis a la expansión de libertad humana para vivir el tipo de vida que la gente considera valedera. Cuando se adopta esa visión mas amplia, el proceso de desarrollo no puede verse simplemente como un incremento del PIB sino como la expansión de la capacidad humana para llevar una vida mas libre y mas digna.In this article the relationships and the differences between the concept of 'human capital' and the concept of 'human capability' are examined. The concept of human capital is more limited since it only conceives human qualities in relation to economic growth, whereas the concept of capabili ties puts emphasis on the expansion of human freedom to live the kind of life that people judge valuable. Whenthis broader vision is adopted, the process of development cannot be seen as simply an increase in the GNP, but rather as the expansion of the human capability to live a more free and worthy life.

  13. Grupo de sala de espera: sentidos do envelhecimento humano

    Directory of Open Access Journals (Sweden)

    Cesar Sandoval Moreira Jr.

    2003-06-01

    Full Text Available Este trabalho parte da consideração do envelhecimento humano como experiência heterogênea. Busca pôr em diálogo o grupo de sala de espera, recorrendo ao construcionismo social, para descrevê-lo como prática discursiva. Problematizando essa modalidade de intervenção psicológica, nesse estudo objetivamos descrever o processo de produção de sentidos sobre o envelhecimento humano nesse contexto. Dez grupos de sala de espera, gravados e transcritos, foram pré-analisados favorecendo a escolha de um deles para análise extensiva. Essa análise apontou que, nesse processo conversacional, a vivência do envelhecimento como experiência individual vai sendo paulatinamente reconstruída pelos interlocutores como de natureza sócio-interacional, apontando para posições mais ativas na produção de qualidade de vida no envelhecimento. Assim, os participantes em co-autoria, falando de seus cotidianos, abrem possibilidades de novas descrições sobre si mesmos enquanto pessoas idosas, nesse contexto interativo.

  14. Gradualismo y equilibrio puntuado en el origen del comportamiento humano

    Directory of Open Access Journals (Sweden)

    Ignacio de la TORRE SÁINZ

    2009-12-01

    Full Text Available RESUMEN: La visión tradicional de la evolución del comportamiento ha sobrevalorado algunos de los aspectos materiales de la cultura concebidos como propiamente humanos. Sin embargo, en los últimos años una serie de estudios han revelado la existencia de estos rasgos adaptativos en otros primates. Esto ha cuestionado seriamente la validez de muchos de las aproximaciones materialistas que parten de la premisa de que sólo lo que aparece en el registro arqueológico es factible de ser interpretado. En este artículo se pasa revista a las explicaciones gradualistas y puntuadas de la aparición del comportamiento humano, y se subraya la necesidad de enfocar el problema desde una consideración socioecológica.ABSTRACT: The traditional views on the evolution of human behavior have overemphasized some of the material aspects of culture, conceived as properly human. However, during the last years, the studies of apes have revealed the existence of these adaptive traits in other primates. This fact has seriously damaged the validity of such a materialist - biased approach (only what is left in the archaeological record is liable to be interpreted as a clear-cut human diagnosis. Gradualist and punctuated explanations of the emergence of human behavior are mentioned as theoretical frameworks and the need of focusing such an issue from a socio-ecological consideration is proposed.

  15. Literatura e direitos humanos na obra de Sacolinha

    Directory of Open Access Journals (Sweden)

    Leila Lehnen

    2016-01-01

    Full Text Available Este artículo examina cómo los textos de escritores de las periferias urbanas brasileñas, específicamente la obra de Ademiro Alves, o Sacolinha, reivindican los derechos humanos de los residentes de la periferia, y por consiguiente articulan su derecho de ser incluidos en la polis a través de la representación tanto de la vida cotidiana como de la injusticia social existente en la periferia urbana brasilera. A partir de la representación de estos planos, que ocurre de forma paralela en los textos aquí examinados, el autor revela las condiciones sociales adversas en las que viven muchos de los residentes de las periferias brasileñas, a la vez que articula una visión positiva de la comunidad y su cultura. Sus textos indican que los habitantes de las periferias no solamente son víctimas de violaciones de derechos humanos, sino que también tienen derechos a tener derechos (Arendt 1951.

  16. POLITICA ECONOMICA, CRECIMIENTO Y DESARROLLO HUMANO: PRINCIPALES RELACIONES

    Directory of Open Access Journals (Sweden)

    Giovanni Reyes

    2008-01-01

    Full Text Available Temas principales en relación con decisiones de política macroeconómica, condiciones económicas nacionales, y nexos de esos tópicos con el crecimiento económico y el desarrollo humano, son abordados aquí, en especial desde la óptica de los países latinoamericanos. En la primera sección, se discuten las principales características de las políticas fiscales, monetarias y cambiarias. Luego, se consideran los enlaces entre esas políticas y el crecimiento de la producción. A partir de allí se exponen los conceptos centrales respecto a desarrollo humano en el contexto específico de las sociedades, tomando en cuenta, el potencial de presentarse la “enfermedad holandesa”. Las principales conclusiones incluyen aquellas que subrayan la necesidad de estabilidad, infraestructura institucional, y condiciones sociales para apoyar el crecimiento económico sostenible y el desarrollo como logros dinámicos y permanentes de una sociedad.

  17. El valor del silencio humano en la cultura escolar

    Directory of Open Access Journals (Sweden)

    J. HUMBERTO MOTTA ÁVILA

    2016-01-01

    Full Text Available El contenido del presente artículo constituye un apartado del marco teórico de la tesis doctoral del autor, intitulada La actitud de escucha en la conversación pedagógica de aula, indagación que se ubica en el interjuego entre el lenguaje y la cultura, y se focaliza hacia las habilidades comunicativas básicas, profundizando en el fenómeno de la escucha que comparten los sujetos educativos estudiante y docente. El opúsculo se propone poner en escena el alcance de la significación que puede tener el silencio humano en el contexto de la cultura escolar actual, específicamente en la vivencia del aula, marcada por la presencia explícita del ruido y la desescucha; abordar el silencio pedagógico como un acto del lenguaje y como fundamento de la escucha áulica; y mostrar que se hace impostergable la resignificación del silencio humano a través de memes que hagan posible su comprensión y puesta en práctica en la conversación pedagógica de aula y en la comunicación cotidiana de los sujetos educativos.

  18. Los Derechos Humanos y desaparecidos en dictaduras militares

    Directory of Open Access Journals (Sweden)

    Juan Carlos GUTIÉRREZ CONTRERAS

    2009-11-01

    Full Text Available RESUMEN: La doctrina de la seguridad nacional jugó un papel predominante como soporte ideológico de las dictaduras del Cono Sur americano. Dentro de este marco se adelantó el "Plan Cóndor" que llevó a la múltiple y sistemática violación de derechos humanos en los países en los que se desarrolló. El artículo hace un análisis jurídico de la responsabilidad de los actores a la luz del derecho penal internacional y del derecho internacional de los derechos humanos, con especial énfasis en la competencia para el juzgamiento de los hechos. Concluye con el análisis del terrorismo de Estado manifestado a través del genocidio, la tortura y las desapariciones forzadas cometidos durante las dictaduras militares.ABSTRACT: The doctrine of national secutiry played a leading role as an ideological endorsement of the South American dictatorships. The Plan Condor was created in this context, and it led to systematic and multiple human rights violations. This article analyzes the responsibility of different actors in the light of International Law, and puts special emphasis in the competence of courts to judge those facts. It concludes with an analysis of state terrorísm, which led to genocide, torture and disapperances under military dictatorships.

  19. Desarrollo humano, eutopía y viejas palabras

    Directory of Open Access Journals (Sweden)

    Chaime Marcuello Servós

    2005-01-01

    Full Text Available El propósito de este artículo es presentar un marco conceptual a la noción de desarrollo humano como parte importante de una vieja pregunta en ocasiones olvidada, censurada y desde hace un tiempo recuperada por las ciencias sociales; esto es, la pregunta por la buena sociedad. Esta cuestión es algo más que un llamado ético o axiológico. Sus repercusiones son hondas y tienen un contenido social, político, económico y jurídico inevitable. El reto es recuperar una pregunta que va más allá del positivismo tecnocientífico que ha hecho de los datos cuantitativos, del economicismo, de los números y de la falta de crítica una nueva forma de alienación. En este aparente viaje a ninguna parte, no basta con reconocerse como copartícipes del destino de Sísifo, tentados por el carpe diem de Baco o hipnotizados por la vocación de Prometeo y Vulcano. El desarrollo humano ha sido un nuevo paradigma y un cambio de referentes, pero también tiene mucho de re-descubrimiento de viejas palabras.

  20. Externalidades del capital humano en México

    Directory of Open Access Journals (Sweden)

    Edna María Villarreal Peralta

    2016-01-01

    Full Text Available El presente trabajo estima las externalidades del capital humano en México em- pleando los enfoques más utilizados en la literatura. Con el propósito de reducir el potencial sesgo de endogenedidad de la escolaridad individual y promedio rea- lizamos estimaciones con variables instrumentales. Para lo cual empleamos como instrumento de la primera la reforma educativa de 1993 que promulgó la obligatoriedad de la educación secundaria. Para la segunda utilizamos la proporción de egresados de educación media superior y superior y la estructura de la población de los estados en un periodo previo. Los resultados muestran que existe una fuer - te relación positiva y estadísticamente significativa entre experiencia, educación individual y educación promedio estatal con los salarios individuales. Asimismo, confirmamos por medio de la aplicación de los diversos enfoques a través de la coincidencia en los resultados encontrados que existen externalidades significativas del capital humano a nivel estatal en México para el periodo 2000-2010.

  1. Derechos humanos y movimientos sociales: experiencia participativa en la universidad

    Directory of Open Access Journals (Sweden)

    Pablo Álvarez Domínguez

    2011-01-01

    Full Text Available Este artículo recoge una serie de una serie de reflexiones teóricas y prácticas, relacionadas con la necesidad de aprender en una sociedad globalizada sobre Derechos Humanos. Reto que ha de ir ligado a un compromiso de acción que debe desarrollarse en cualquiera de las esferas sociales (movimientos, asociaciones, ONG's, etc. que tenemos a nuestro alcance. Asumimos que en el contexto del proceso de enseñanza y aprendizaje en el contexto universitario, el tema de los Derechos Humanos es una asignatura pendiente a la que ha de dar respuesta la Universidad como institución al servicio de la sociedad y promotora del desarrollo de la justicia social. La enseñanza y el aprendizaje de estos derechos no consiste sólo en darlos a conocer, sino en asimilarlos, asumirlos e integrarlos en el proceso formativo personal, posibilitando que las personas sientan responsabilidad por su desarrollo. Para fomentar la participación social es importante motivarlas desde las emociones, para que sean capaces de implicarse en las circunstancias políticas, sociales y culturales de la sociedad civil. En este trabajo, y con el fin de fundamentar empíricamente nuestro planteamiento, describimos el desarrollo de una experiencia universitaria de participación socioeducativa que nos permite valorar el papel de los movimientos sociales en nuestro actual contexto sociocultural.

  2. Gestión del talento humano y cultura organizacional en el área de recursos humanos de la UGEL 06 – Ate Vitarte, 2016

    OpenAIRE

    Angeles Macavilca, Alberto Reynoldi

    2017-01-01

    La investigación titulada “Gestión del talento humano y cultura organizacional en el área de recursos humanos de la UGEL 06 – Ate Vitarte, 2016”, tuvo como objetivo general de establecer la relación que existe entre la gestión del talento humano y cultura organizacional en el área de recursos humanos de la UGEL 06 - Ate Vitarte, 2016. La investigación se realizó bajo el enfoque cuantitativo y método hipotético deductivo con un tipo de investigación básica y nivel descriptivo correlacional. El...

  3. Visión anatomo-mecánica del cuerpo humano en Descartes

    OpenAIRE

    Idoate García, Victor M.

    1999-01-01

    Pese a que Descartes concibe al cuerpo humano como constituido por un cuerpo (res extensa) y una alma (res cogitans), en este trabajo sólo se considerará la parte que corresponde a la descripción anatómico y mecánica del cuerpo humano.

  4. Estudio de las principales variables en la gestión de los Recursos Humanos

    OpenAIRE

    Losada Rastrilla, Begoña

    2014-01-01

    Se pretende un estudio de las principales funciones en la gestión de los recursos humanos de una empresa, así como la evaluación de una encuesta de satisfacción en los empleados, así como el análisis de las principales variables en empresa. Grado en Relaciones Laborales y Recursos Humanos

  5. Migrantes deportados: entre la concepción de los desechos humanos y la de los derechos humanos

    Directory of Open Access Journals (Sweden)

    Joaquín A. Mejía R.

    2011-08-01

    Full Text Available La crisis económica mundial está provocando una crisis de los valores democráticos en las sociedades del Norte cuando se trata de abordar el fenómeno migratorio.Cada vez más los Estados de Europa y Estados Unidos ven en la migración un asunto que se desecha cuando ya no es útil ni rentable y los derechos humanos de los inmigrantes son sacrificados en el altar de la seguridad nacional, del orden público y de la recesión económica. De esta manera, en nombre de su soberanía, los Estadosaplican políticas migratorias que atentan contra los derechos de las poblaciones migrantes sujetas a deportación, negándoles el derecho básico a ser oído por un juez independiente e imparcial. El objetivo de este artículo es mostrar que cuando un Estado falla en su obligación de respetar y garantizar los derechos humanos,se pueden activar los sistemas de protección internacional para revertir decisiones estatales que responden a una política migratoria discriminatoria y contraria a la dignidad humana.

  6. La alfabetización informacional como derecho humano

    Directory of Open Access Journals (Sweden)

    Paul Sturges

    2012-03-01

    Full Text Available Se puede argumentar que existe una relación entre el derecho a la libertad intelectual (argumento pasivo propuesto por el Artículo 19 de la Declaración Universal sobre los Derechos Humanos de las Naciones Unidas y la consecuente responsabilidad por parte de los gobiernos, los profesionales y los defensores de los derechos civiles de crear (argumento activo las condiciones idóneas para el ejercicio eficaz de la libertad intelectual. Cada vez más los analistas del papel de los medios de comunicación en la sociedad y de la responsabilidad social de la informática también están llegando a conclusiones de este tipo, resaltando la importancia tanto de la alfabetización mediática como de la alfabetización en informática. Obviamente, este argumento nos lleva a analizar el planteamiento que los profesionales de la información se hacen de los cursos sobre alfabetización de información que actualmente imparten. Aunque existen muchos programas de alfabetización informacional que son muy completos, éstos, hasta ahora, se basan, en su mayor parte, en lo que los profesionales piensan que son las necesidades prácticas de los usuarios. Sin embargo, se puede interpretar que las declaraciones de tipo general como, por ejemplo, la Declaración de Praga “Hacia una Sociedad de la Alfabetización Informacional” del 2003 y la Proclamación de Alejandría del 2005 empiezan a apuntar hacia una justificación de las actividades de la alfabetización informacional basadas en los derechos humanos, en concreto en el artículo 19. El argumento es que si se parte de una perspectiva basada en los derechos humanos podemos llegar a una interpretación sólida de la alfabetización informacional que incluya la alfabetización mediática, la alfabetización en informática, la alfabetización en la web y, hasta cierto punto, la alfabetización cívica, desarrollando de este modo un modelo que responde a las necesidades humanas más que a las prioridades

  7. Influenza A(H1N1)pdm09 in critically ill children admitted to a ...

    African Journals Online (AJOL)

    positive for the following respiratory viruses: H1N1, seasonal influenza. A, influenza B, parainfluenza 1/2/3, rhinovirus, respiratory syncytial virus (RSV), human metapneumovirus (HMPV) and adenovirus. Patients were tested for the above respiratory viruses if they met the clinical case definition for a moderate or severe ...

  8. UMA CRISE DO CAPITAL HUMANO NA ECONOMIA BRASILEIRA

    Directory of Open Access Journals (Sweden)

    Carlos Antonio Gomes

    2013-06-01

    Full Text Available No sistema produtivo, meios de comunicação e nas instituições representativas do capital, é voz corrente que o Brasil vive uma vive uma crise de mão de obra qualificada. Embora os estudos sobre o trabalho tenham dificuldade em reconhecer o fato, inúmeras pesquisas confirmam que o país vive uma crise do capital humano. Esse trabalho apresenta um breve diagnóstico da crise e da estrutura de educação profissional, confirmando que problema encerra uma séria ameaça concreta à economia, desenvolvimento e ao processo de mobilidade social que ora se desenrola no Brasil.

  9. A ética ambiental dos direitos humanos

    Directory of Open Access Journals (Sweden)

    João Paulo Miranda

    2016-07-01

    Full Text Available Este trabalho visa abordar os modelos éticos de proteção jurídica do meio ambiente, desde a análise do biocentrismo ao antropocentrismo mitigado e intergeracional que fundamenta o conceito de sustentabilidade. Inicialmente é abordado a natureza jusfundamental e de direitos humanos do meio ambiente. Em seguida, são apresentados três modelos éticos de proteção do meio ambiente, que orientam as normativas de proteção ambiental, o não-antropocentrismo; antropocentrismo puro; e antropocentrismo mitigado. A partir disto, é analisado a evolução da vertente não antropocêntrica, passando pelo biocentrismo, pelo ecocentrismo, e pela ecologia profunda, até a um antropocentrismo, inicialmente puro, que é mitigado pelos aspectos intergeracionais, intrínsecos à natureza difusa do bem ambiental, como modelo atual vigente.

  10. Los gestionadores del recurso humano en el mercado de trabajo

    Directory of Open Access Journals (Sweden)

    Jesús Castañeda Palacios

    1991-05-01

    Full Text Available RESUMEN El artículo presenta las diferentes alternativas cuando se consulta a los gestionadores, que participan en  el Mercado de Trabajo, para la consecución del recurso humano, con la finalidad de llenar las vacancias existentes en las empresas. Una segunda óptica apunta a visualizar la intervención del Estado, en el campo de la intermediación Laboral y la relación con los particulares que ejercen esta actividad. Finalmente, se plantean algunas tendencias sobre la manera como se desenvolverán esta clase de organizaciones a la Luz de la ley 50 de 1990 (Reforma laboral.

  11. Un malestar invisible: derechos humanos y comunicación

    Directory of Open Access Journals (Sweden)

    Rossana Reguillo

    2015-01-01

    Full Text Available La relación entre derechos humanos y comunicación alude a una relación cultura, se trata fundamentalmente del debate entre la inclusión y la exclusión por lo que se impone un trabajo para el desmontaje crítico de los dispositivos, discursos y mecanismos que naturalizan la exclusión y expropian la posibilidad de la palabra a las mujeres, indígenas y jóvenes...Para analizar el modo en que éstos son construidos y representados en el espacio público, la autora recurre a tres estrategias: el desmontaje de una expresión popular, la crítica a un pensamiento legitimado y el uso del relato etnográfico.

  12. ECONOMIA DOS RECURSOS HUMANOS: TEORIA E EVIDÊNCIAS

    Directory of Open Access Journals (Sweden)

    Andre Carraro

    2014-12-01

    Full Text Available Este trabalho tem como objetivo discutir as diversas contribuições dadas ao longo das últimas décadas à economia dos recursos humanos, uma área de crescente destaque tanto entre a comunidade acadêmica, quanto entre empresários e trabalhadores. Discutem-se os diversos tópicos que compõem as abrangentes relações empresariais, estabelecidos na literatura em quatro tópicos principais: treinamento, incentivos, organização e seleção de pessoas. É feita uma análise dos principais artigos e autores de cada uma dessas subáreas, com o intuito principal de facilitar o acesso à informação, apontar caminhos para novas pesquisas e incentivar potenciais pesquisadores.

  13. SEGURIDAD EN ACTIVOS DE INFORMACIÓN HUMANOS

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    Jorge Mario Cadavid-Aguirre

    2013-07-01

    Full Text Available Las empresas invierten grandes cantidades de dinero en estrategias de seguridad orientadas al control tecnológico: Antivirus, FireWall, Cifrado, IPS, IDS , pero a pesar de todos esos esfuerzos siguen siendo vulneradas. El problema radica en que dichas tecnologías no cubren el activo que más interactúa con la información; El ser humano. Está ponencia tuvo como objetivo concientizar al usuario final, hacerle ver la importancia de sus hábitos y comportamientos para mantener niveles seguros en su información sensible tanto empresarial como personal. Al final de la charla, se realizaron laboratorios prácticos con el fin de mostrar cómo operan los ciberdelincuentes y las técnicas de hacking más comunes de ataque al usuario final.

  14. O capital humano e o desenvolvimento econômico

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    Beatriz Maria Witkowski

    2007-07-01

    Full Text Available Um ambiente competitivo de negócios é decorrência cada vez maior de uma administração eficaz do capital humano. Estrutura, tecnologia, recursos financeiros e materiais são aspectos meramente físicos e inertes que precisam ser administrados inteligentemente por meio das pessoas que constituem a organização. A Teoria do Capital Humano é uma derivação da Teoria Econômica Neoclássica e, ao mesmo tempo, uma atualização, do axioma liberal do indivíduo livre, soberano e racional. Ressurgiu com a crise do modelo tayloris ta-fordis ta, associada à redefinição das relações de trabalho na empresa e do papel do sistema educacional. A qualidade dos funcionários de uma organização, seus conhecimentos e habilidades, entusiasmo e satisfação com seus cargos, seu senso de iniciativa para gerar riqueza, tudo isso tem forte impacto na produtividade da organização, no nível de serviço ao cliente, na reputação e na competitividade. Para qualquer que seja a área empresarial escolhida, o futuro administrador necessita ter uma visão sobre como lidar com assuntos relacionados com pessoas para alcançar o seu sucesso profissional e levar a sua organização rumo à excelência e à competitividade. Em seu conjunto, as pessoas constituem o capital humano da organização. Esse capital pode valer mais ou valer menos, na medida em que contenha talentos capazes de agregar valor à organização e torná-la mais ágil e competitiva. Portanto, esse capital vale mais na medida em que consiga influenciar as ações e os destinos da organização. A era da informação torna o trabalho menos físico e mais mental, pois as pessoas deixaram de ser fornecedoras de mão-de-obra para serem alçadas à categoria de fornecedoras de conhecimento e de competências. Do mesmo modo que o capital humano é relevante para a sociedade e para as organizações, observa-se que o desenvolvimento econômico também possui um papel de suma importância. Define

  15. Ciudadanía, universidad y Derechos Humanos

    Directory of Open Access Journals (Sweden)

    Juan BENITO MARTÍNEZ

    2011-01-01

    Full Text Available Hablar de pedagogía de la sociedad civil a favor de los derechos humanos desde la Universidad supone evaluar sus auténticas posibilidades de educar en la ciudadanía democrática, de puertas adentro y de puertas afuera, mediante la transformación del propio currículum y el diseño de proyectos estratégicos en asociación directa con los agentes comunitarios. Es claro que el desarrollo pleno de las sociedades democráticas requiere, inexorablemente, ciudadanos con valores éticos, responsabilidad social y competencias cívicas, con la plena conciencia de los problemas culturales, ambientales y sociales que nos afectan. En este ambicioso reto, las universidades deben ser entes creadores de cultura, espacios para la participación y motor de innovación.

  16. Condiciones para una gestión estratégica de recursos humanos

    OpenAIRE

    González Fernández, Luis

    2004-01-01

    [ES]La década de los años noventa supone la incorporación del punto de vista estratégico en la gestión de recursos humanos. Frente a esta nueva perspectiva las prácticas tradicionales de gestión de recursos humanos deben efectuar una profunda transformación de cara a convertirse en una fuente de ventaja competitiva sostenible para la empresa. Los métodos, las herramientas utilizadas hasta ahora para la gestión de recursos humanos se revelan obsoletas y sin impacto relevante sobre el desempeño...

  17. Tecnología para el desarrollo humano, del Dr. Luis Camacho Naranjo

    OpenAIRE

    Vargas, Celso; Escuela de Ciencias Sociales, Instituto Tecnológico de Costa Rica

    2013-01-01

    La reseña de la obra Tecnología para el desarrollo humano, del Dr. Luis Camacho, y publicada por la Editorial Tecnológica (Editorial del Instituto Tecnológico de Costa Rica) en el año 2005, es abordada por Vargas desde dos perspectivas: como obra de importancia que se suma a la bibliografía sobre temas de desarrollo humano y como aporte al enfoque del desarrollo humano desde la reflexión ética. Se reseñan los conceptos esenciales que se tratan a lo largo de los tres capítulos del libro: desar...

  18. El proceso de selección de talento humano bajo el enfoque de competencias

    OpenAIRE

    Moreira de Vélez, Janeth; Loor Zambrano, Halder; Velásquez Velásquez, María

    2013-01-01

    El proceso de selección de talento humano por competencias no es sólo una de las fases del modelo de gestión por competencias, si no la más importante de las etapas, justamente porque de éste proceso depende la efectividad del cumplimiento de los resultados en las distintas áreas de la organización, a saber, finanzas, talento humano, producción y operaciones, marketing, administración y hasta la propia área de talento humano. En el transcurso de esta investigación, se emplean técnicas como la...

  19. Breve recorrido histórico de la educación en derechos humanos

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    Janette Góngora Soberanes

    2015-01-01

    Full Text Available La educación en derechos humanos es primordial para el desarrollo íntegro de las personas y una base necesaria para cualquier proceso de democratización de una sociedad. Es una herramienta para la prevención de las violaciones de los derechos humanos, fomenta conductas y actitudes basadas en la tolerancia, solidaridad y respeto, y disminuye los graves problemas de violencia, resentimiento y discriminación. En el presente artículo se desarrolla un recorrido histórico de la educación en derechos humanos.

  20. Teoria do Capital Humano: Breve Discussão Teórica

    OpenAIRE

    Rosângela Saldanha Pereira

    2011-01-01

    Neste texto exploramos os fundamentos teóricos da teoria do capital humano, a sua crítica aos pressupostos da teoria clássica e concluímos que a teoria do capital humano é um desdobramento singular dos postulados da teoria econômica marginalista aplicados à educação. Não objetivamos fazer um tratado sobre as teorias aqui abordadas. Estamos buscando apenas apresentar os vínculos do capital humano com a visão marginalista e destacar o avanço proporcionado por esta abordagem para a compreensão ...

  1. Diversidad cultural y derechos humanos desde los referentes cosmovisionales de los pueblos indígenas

    OpenAIRE

    Gómez-Isa, F. (Felipe)

    2011-01-01

    Lejos de suponer un ataque a los derechos humanos de carácter universal, la creciente diversidad cultural puede suponer un enriquecimiento de los mismos. El progresivo reconocimiento de los derechos de los pueblos indígenas está impulsando, aunque no sin dificultades, una concepción más abierta, más dinámica y más inclusiva de los derechos humanos. Tanto la Corte Constitucional de Colombia como la Corte Inter-Americana de Derechos Humanos están contribuyendo decisivamente con su progresi...

  2. La seguridad en la aviación depende de los factores humanos

    Directory of Open Access Journals (Sweden)

    Esperanza Lozano Alvernia

    2008-01-01

    Full Text Available En este articulo se hace un abordaje se.ncil lo de lo que significan los factores humanos para la seguridad en la aviación visto desde la ca lidad integral, de ahí que se hace una reflexión de lo que implica la calidad en las instituciones para partir de este concepto y desarrollar los aspectos de los factores humanos, de las disciplinas que orientan el estudio de los factores huma.nos, y el entrenamiento que debe recibir la tripulación de vuelo según la OACI.

  3. Principales prácticas de recursos humanos de las PyMEs industriales exitosas

    OpenAIRE

    López-Suárez, Liliana-Rocío; Tricás Preckler, Jesús; Toledano O’Farril, Rubén

    2013-01-01

    El éxito competitivo de la PyME depende en parte de la buena gestión de recursos humanos,por lo que no darle el valor que requiere constituye una de las principales causas de fracasode estas empresas. La responsabilidad de la gestión eficaz de los recursos humanos recae fundamentalmenteen los directivos. La presente investigación tiene como objetivo identificarlos estilos de dirección y las principales prácticas de recursos humanos que llevan a cabo lasPyMEs industriales exitosas de México y ...

  4. Hermenéutica de los derechos humanos en la obra de Ana Luisa Guerrero

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    Orlando Inocencio Aguirre Martínez

    2015-06-01

    Full Text Available La obra de Ana Luisa Guerrero se caracteriza por su abordaje filosófico-político sobre los derechos humanos y su aplicación. Su hermenéutica intercultural, dirigida, principalmente, a los derechos humanos de tercera generación, identifica el componente de la intersubjetividad que encamina hacia la construcción de un diálogo intercultural. Este componente permite, además, discutir sobre los fundamentos de los derechos humanos colocando las percepciones individuales y colectivas del paradigma.

  5. A emergência da responsabilidade criminal individual no Sistema Interamericano de Direitos Humanos

    OpenAIRE

    Lima,Raquel da Cruz

    2012-01-01

    Este artigo analisa a emergência e difusão da regra da responsabilidade criminal individual por violações de direitos humanos a partir da jurisprudência contenciosa da Corte Interamericana de Direitos Humanos. Tendo como referência as pesquisas de Kathryn Sikkink sobre justiça de transição, procura-se identificar a maneira como o Sistema Interamericano de Direitos Humanos vem expandindo o sentido da responsabilidade criminal individual e também sugerir a incompatibilidade com a proteção dos d...

  6. La dignidad del embrión humano. ¿Qué sentido tiene que se le niegue al embrión humano el derecho a vivir?

    Directory of Open Access Journals (Sweden)

    Juan de Dios Vial Correa

    2009-06-01

    Full Text Available El concepto de “dignidad humana” ha ido evolucionando en la historia de la culturaoccidental hasta verla como algo intrínseco a la persona humana y no vinculado a una o más de las cualidades del ser humano con más o menos gradación; “todas las cosas tienen precio; pero el hombre tiene dignidad”. Más bien, es propio cuando el hombre pierde sus cualidades físicas, intelectuales, morales que resplandece más su pura dignidad. Por tanto, si el embrión es un ser humano (porque ha empezado un desarrollo específicamente humano se le debe el mismo respeto que a un adulto. Negarle este respeto, como ocurre con frecuencia, significa haber elegido una ética racionalista, utilitarista, de búsqueda de consensos sociales, una ética que ha reducido el ser humano a objeto. Sólo una concepción del ser humano como ser abierto al Absoluto, un “yo inhabitado por Dios” (F. Rielo tendrá la garantía de ser respetado en su dignidad, siempre y por todos.

  7. Comparative Evaluation Of Conventional Rt-pcr And Real-time Rt-pcr (rrt-pcr) For Detection Of Avian Metapneumovirus Subtype A [comparação Entre As Técnicas De Rt-pcr Convencional E Rt-pcr Em Tempo Real Para A Detecção Do Metapneumovírus Aviários Subtipo A

    OpenAIRE

    Ferreira H.L.; Spilki F.R.; dos Santos M.M.A.B.; de Almeida R.S.; Arns C.W.

    2009-01-01

    Avian metapneumovirus (AMPV) belongs to Metapneumovirus genus of Paramyxoviridae family. Virus isolation, serology, and detection of genomic RNA are used as diagnostic methods for AMPV. The aim of the present study was to compare the detection of six subgroup A AMPV isolates (AMPV/A) viral RNA by using different conventional and real time RT-PCR methods. Two new RT-PCR tests and two real time RT-PCR tests, both detecting fusion (F) gene and nucleocapsid (N) gene were compared with an establis...

  8. Dirección de recursos humanos y competitividad

    Directory of Open Access Journals (Sweden)

    Gregorio Calderón Hernandez

    2003-09-01

    Full Text Available La Dirección de Recursos Humanos (DRH es una capacidad organizacional clasificada dentro de los activos intangibles y como tal puede convertirse en apoyo importante en la construcción de ventaja competitiva sostenida para la empresa. El presente estudio tiene el propósito de establecer hasta qué punto la DRH se constituye en soporte de la empresa colombiana para alcanzar dicha ventaja competitiva. La base empírica para la elaboración del artículo es información proveniente de la investigación "La gerencia de talento humano en las empresas medianas y grandes del occidente colombiano", en la que se encuestaron gerentes de recursos humanos de 257 empresas. Se consideraron las variables: grado de desarrollo de la DRH, ubicación del área en la estructura jerárquica, tamaño de la organización, sector económico, tipo de dirección, políticas de personal, factores claves de éxito y problemas en la gestión de RH. El grado de desarrollo de la DRH se estableció a partir de nueve componentes: percepción de autonomía del director de RH, asignación presupuestal al área, nivel educativo del director, nivel de liderazgo del director, existencia de plan estratégico en el área, estrategias innovadoras que el área ha propuesto en los últimos dos años, tiempo que el director debe dedicar a actividades no operativas ni rutinarias e indicadores de gestión que emplea el área de RH. La revisión de la literatura especializada permite concluir que los RH serán una fuente de ventaja competitiva sostenida siempre y cuando sean valiosos, escasos, relativamente inimitables e insustituibles, pero que no basta con ello si la firma carece de la organización para capitalizar el valor potencial de la gente. Esta es una capacidad que le corresponde a la DRH, que de esta manera se convierte en elemento clave para darle a los RH su característica de ventaja competitiva sostenida. Los hallazgos demuestran que el grado de desarrollo de la DRH est

  9. Pluralismo Jurídico y Derechos Humanos

    Directory of Open Access Journals (Sweden)

    Alexei Julio-Estrada

    2013-06-01

    1. Modelos de Estados. Los diversos modelos de Estado no sólo tienen fundamentos distintos, sino que también consecuencias diferentes respecto de su estructura institucional y cultural, ¿cuál es, a su juicio, el modelo de Estado (liberal-social-multicultural plurinacional que permite resguardar u otorgar un mejor reconocimiento a los derechos indígenas? Y ¿cuáles son las consecuencias de tratar el multiculturalismo bajo este modelo estatal?2. Modelo institucional. La construcción institucional de un Estado (poderes, funciones, relación entre los poderes, relación con la sociedad civil, entre otros aspectos es fundamental para llevar a cabo las ideas fundantes de un determinado modelo estatal. En este sentido, se ha discutido sobrela necesidad de replantear la institucionalidad una vez reconocida la multiculturalidad (nuevos poderes, nuevas relaciones entre poderes, redefinición de funciones, nuevas formas de participación. A su juicio, ¿es necesario efectuar ajustes en el modelo institucional o cabe rediseñarlo para dar reconocimiento y garantía a los derechos indígenas?3. Aporte del DIDH. El DIDH ha aportado en el reconocimiento y garantía de los derechos indígenas y de aquellos de los pueblos indígenas. A su juicio, ¿de qué forma el DIDH ha sido significativo para el avance en materia de reconocimiento de una perspectiva amplia del contenido y alcance de los derechos humanos de titulares indígenas? ¿Qué rol juega en dicho proceso el reconocimiento de los derechos colectivos?4. Límites a las prácticas culturales indígenas. Desde una perspectiva de respeto a la dignidad y los derechos humanos consagrados en las constituciones nacionales y los tratados internacionales: ¿es posible y/o justificable imponer límites a las prácticas culturales indígenas o sistemas legales indígenas?

  10. El origen no humano de la música

    Directory of Open Access Journals (Sweden)

    Juan David Leongómez

    2008-09-01

    Full Text Available Resumen: Si la música tiene un origen cultural -como suele asumirse- ¿cómo puede explicarse su universalidad? Este artículo parte de este problema para plantear la hipótesis de que el fenómeno musical tenga un origen anterior al hombre moderno, o incluso anterior al hombre mismo y sea el resultado de una serie de adaptaciones al medio; de que tenga un origen biológico y no cultural. Tras analizar diversos estudios en áreas tan diversas como la semiótica, la neurología, la etología y la evolución, en los que se ve que muchas especies animales compartimos la capacidad de reconocer y entender signos, las estructuras y capacidades necesarias para su percepción, el placer y potencialmente la producción de memorias e imágenes metafóricas que se evocan cuando escuchamos música, los procesos culturales implicados, y que en muchos casos nos comunicamos acústicamente con los mismos objetivos e incluso sobre los mismos patrones universales que los humanos usamos al hacer música, se llega a la conclusión de que es posible que la música tenga un origen no humano y que sea producto de adaptaciones evolutivas, y a la sugestiva idea de que el origen de este fenómeno -es decir el desarrollo de las estructuras básicas implicadas en los procesos musicales- pueda rastrearse teóricamente incluso hasta los reptiles. Abstract: If music has a cultural origin -as is often assumed- how can one explain its universality? This paper is based on this question to raise the hypothesis that the musical phenomenon has its origin before the arising of modern man, or even before the evolution of man himself and, therefore, is the result of a series of adaptations to the environment, having a biological rather than a cultural origin. After considering further studies in areas as diverse as Semiotics, Neuroscience, Ethology and Evolution, showing that the physiological, behavioural, cognitive and perceptual characteristics related to the musical production

  11. El proceso de selección de talento humano bajo el enfoque de competencias

    Directory of Open Access Journals (Sweden)

    Janeth Moreira de Vélez

    2013-12-01

    Full Text Available El proceso de selección de talento humano por competencias no es sólo una de las fases del modelo de gestión por competencias, si no la más importante de las etapas, justamente porque de éste proceso depende la efectividad del cumplimiento de los resultados en las distintas áreas de la organización, a saber, finanzas, talento humano, producción y operaciones, marketing, administración y hasta la propia área de talento humano. En el transcurso de esta investigación, se emplean técnicas como la Entrevista de Eventos Conductuales (EEC, Assessments o Centros de Evaluación, y la aplicación de Pruebas Psicotécnicas. PALABRAS CLAVES: Competencia organizacional, competencia técnica, assessments, selección, talento humano, evento conductual.

  12. Justicia y derechos humanos: posibilidades de una reflexión desde los planteamientos rawlsianos

    Directory of Open Access Journals (Sweden)

    Paulina Morales Aguilera

    2009-01-01

    Full Text Available El presente artículo brinda una reflexión sobre los derechos humanos a la luz del pensamiento de John Rawls y sus planteamientos en torno a la justicia. En efecto, hablar de derechos humanos es hacer referencia indiscutiblemente a una noción de justicia que les subyace.Y hablar de justicia nos remite, indefectiblemente, a Rawls, quien en 1971 estremece el ambiente filosófico con su ya famosa Teoría de la justicia. De ella se intenta rescatar aportes para una lectura de los derechos humanos, especialmente en relación con los principios de una justicia como equidad y con las posibilidades de configurar un sujeto de derechos al amparo de la consideración de la personalidad moral del ser humano.

  13. Derechos humanos y criminología: un vínculo ignorado

    Directory of Open Access Journals (Sweden)

    Mario Arroyo Juárez

    2002-01-01

    Full Text Available Este trabajo busca analizar algunas contradicciones presentes en el discurso de los derechos humanos, y cuestionar la visión cronológica generalmente aceptada, que lleva a algunos autores a suponer que existe sólo un discurso sobre los derechos humanos. La breve revisión sobre la génesis y el desarrollo histórico de dicho discurso tiene por objeto discutir una nueva tipologia que permita distinguir y estudiar los diferentes discursos sobre derechos humanos que hoy se utilizan. Una segunda intención es demostrar cómo la criminología y la victimología, con restricciones menores, son discursos que pueden aportar conceptos útiles para una mejor comprensión y explicación de lo que acontece en torno a los derechos humanos.

  14. Principales prácticas de recursos humanos de las PyMEs industriales exitosas.

    Directory of Open Access Journals (Sweden)

    Liliana Rocío López Suárez

    2013-04-01

    Full Text Available El éxito competitivo de la PyME depende en parte de la buena gestión de recursos humanos,por lo que no darle el valor que requiere constituye una de las principales causas de fracasode estas empresas. La responsabilidad de la gestión eficaz de los recursos humanos recae fundamentalmenteen los directivos. La presente investigación tiene como objetivo identificarlos estilos de dirección y las principales prácticas de recursos humanos que llevan a cabo lasPyMEs industriales exitosas de México y España. Para alcanzar los objetivos antes reseñados,se ha realizado un análisis documental, así como entrevistas en profundidad a expertosen el área de recursos humanos. En este trabajo se presentan los principales resultados conrespecto a las prácticas mencionadas.

  15. PERSPECTIVAS SANITARIAS PROMETEDORAS DEL CLONAJE HUMANO PERSPECTIVAS SANITARIAS PROMETEDORAS DA CLONAGEM HUMANO PROMISING HEALTH PERSPECTIVES FOR HUMAN CLONING

    Directory of Open Access Journals (Sweden)

    Fermín Rolando Schramm

    2003-01-01

    Full Text Available Para evaluar las promesas del clonaje humano -tanto las terapéuticas como las reproductivas- se puede partir de dos puntos de vista pertinentes: el sanitario y el de la bioética laica. El punto de vista sanitario, a través de políticas públicas de prevención y de promoción de salud adecuadas a las condiciones objetivas existentes, busca proteger la población humana de enfermedades innecesarias. El clonaje puede, en principio, ser parte de una política sanitaria, con la condición de que sean respetados los derechos fundamentales del individuo -en particular el derecho a la autodeterminación personal-, los derechos sociales -como la justicia distributiva- y los derechos de tercera generación -que incluyen medidas protectoras de bioseguridad. La bioética laica busca entender la eticidad del clonaje por medio del análisis racional e imparcial de las implicaciones morales de su uso y, si se considera legítimo, proponerlo como medio para proteger la salud humana. A pesar de sus funciones aparentemente muy diferentes, tanto el clonaje terapéutico como el reproductivo buscan responder al desafío del sufrimiento humano innecesario: el clonaje terapéutico, gracias a la técnica que utiliza célulasmadre o estaminales totipotentes, busca producir órganos y tejidos para eliminar el sufrimiento evitable; el clonaje reproductivo puede también paliar formas de sufrimiento humano innecesario y puede, por ende, ser considerado un caso particular del terapéuticoPara avaliar corretamente as promessas da clonagem humana, tanto as terapêuticas como as reprodutivas, podese partir de dois pontos de vista pertinentes: o ponto de vista sanitário e o ponto de vista da bioética laica. O ponto de vista sanitário, graças a políticas públicas de prevenção e promoção da saúde adequadas às condições objetivas existentes, visa proteger a população humana do adoecimento não necessário. A bioética laica procura entender a eticidade da

  16. Laboratory evaluation of a quantitative real-time reverse transcription PCR assay for the detection and identification of the four subgroups of avian metapneumovirus.

    Science.gov (United States)

    Guionie, O; Toquin, D; Sellal, E; Bouley, S; Zwingelstein, F; Allée, C; Bougeard, S; Lemière, S; Eterradossi, N

    2007-02-01

    Avian metapneumovirus (AMPV) is an important pathogen causing respiratory diseases and egg drops in several avian species. Four AMPV subgroups have been identified. The laboratory diagnosis of AMPV infections relies on serological methods, on labour-intensive virus isolation procedures, and on recently developed subgroup specific reverse transcription PCR (RT-PCR) protocols. In the present study, both the specificity and sensitivity of a commercial real-time reverse transcription PCR (RRT-PCR) for the detection and identification of the four AMPV subgroups were evaluated. Fifteen non-AMPV avian viruses belonging to 7 genera and 32 AMPV belonging to the 4 subgroups were tested. No non-AMPV virus was detected, whereas all AMPV viruses were identified in agreement with their previous molecular and antigenic subgroup assignment. The sensitivity and quantitating ability of the RRT-PCR assay were determined using serial dilutions of RNA derived either from AMPV virus stocks or from runoff transcripts. In all cases, linear dose/responses were observed. The detection limits of the different subgroups ranged from 500 to 5000 RNA copies and from 0.03 to 3.16TCID50/ml. The results were reproducible under laboratory conditions, thus showing that quantitative RRT-PCR is a new and powerful tool for the rapid and sensitive detection, identification and quantitation of AMPVs.

  17. Immune responses and interactions following simultaneous application of live Newcastle disease, infectious bronchitis and avian metapneumovirus vaccines in specific-pathogen-free chicks.

    Science.gov (United States)

    Awad, Faez; Forrester, Anne; Baylis, Matthew; Lemiere, Stephane; Jones, Richard; Ganapathy, Kannan

    2015-02-01

    Interactions between live Newcastle disease virus (NDV), avian metapneumovirus (aMPV) and infectious bronchitis virus (IBV) vaccines following simultaneous vaccination of day old specific pathogen free (SPF) chicks were evaluated. The chicks were divided into eight groups: seven vaccinated against NDV, aMPV and IBV (single, dual or triple) and one unvaccinated as control. Haemagglutination inhibition (HI) NDV antibody titres were similar across all groups but were above protective titres. aMPV vaccine when given with other live vaccines suppressed levels of aMPV enzyme-linked immunosorbent assay (ELISA) antibodies. Cellular and local immunity induced by administration of NDV, aMPV or IBV vaccines (individually or together) showed significant increase in CD4+, CD8+ and IgA bearing B-cells in the trachea compared to the unvaccinated group. Differences between the vaccinated groups were insignificant. Simultaneous vaccination with live NDV, aMPV and IBV did not affect the protection conferred against aMPV or IBV. Copyright © 2014 Elsevier Ltd. All rights reserved.

  18. Development of a Nucleoprotein-Based Enzyme-Linked Immunosorbent Assay Using a Synthetic Peptide Antigen for Detection of Avian Metapneumovirus Antibodies in Turkey Sera

    Science.gov (United States)

    Alvarez, Rene; Njenga, M. Kariuki; Scott, Melissa; Seal, Bruce S.

    2004-01-01

    Avian metapneumoviruses (aMPV) cause an upper respiratory tract disease with low mortality but high morbidity, primarily in commercial turkeys, that can be exacerbated by secondary infections. There are three types of aMPV, of which type C is found only in the United States. The aMPV nucleoprotein (N) amino acid sequences of serotypes A, B, and C were aligned for comparative analysis. On the basis of the predicted antigenicity of consensus sequences, five aMPV-specific N peptides were synthesized for development of a peptide antigen enzyme-linked immunosorbent assay (aMPV N peptide-based ELISA) to detect aMPV-specific antibodies among turkeys. Sera from naturally and experimentally infected turkeys were used to demonstrate the presence of antibodies reactive to the chemically synthesized aMPV N peptides. Subsequently, aMPV N peptide 1, which had the sequence 10-DLSYKHAILKESQYTIKRDV-29, with variations at only three amino acids among aMPV serotypes, was evaluated as a universal aMPV ELISA antigen. Data obtained with the peptide-based ELISA correlated positively with total aMPV viral antigen-based ELISAs, and the peptide ELISA provided higher optical density readings. The results indicated that aMPV N peptide 1 can be used as a universal ELISA antigen to detect antibodies for all aMPV serotypes. PMID:15013970

  19. Effects of cyclosporin A induced T-lymphocyte depletion on the course of avian Metapneumovirus (aMPV) infection in turkeys.

    Science.gov (United States)

    Rubbenstroth, Dennis; Dalgaard, Tina S; Kothlow, Sonja; Juul-Madsen, Helle R; Rautenschlein, Silke

    2010-05-01

    The avian Metapneumovirus (aMPV) causes an economically important acute respiratory disease in turkeys (turkey rhinotracheitis, TRT). While antibodies were shown to be insufficient for protection against aMPV-infection, the role of T-lymphocytes in the control of aMPV-infection is not clear. In this study we investigated the role of T-lymphocytes in aMPV-pathogenesis in a T-cell-suppression model in turkeys. T-cell-intact turkeys and turkeys partly depleted of functional CD4(+) and CD8(+) T-lymphocytes by Cyclosporin A (CsA) treatment were inoculated with the virulent aMPV subtype A strain BUT 8544. CsA-treatment resulted in a significant reduction of absolute numbers of circulating CD4(+) and CD8alpha(+) T-lymphocytes by up to 82 and 65%, respectively (P<0.05). Proportions of proliferating T-cells within mitogen-stimulated peripheral blood mononuclear cells were reduced by similar levels in CsA-treated birds compared to untreated controls (P<0.05). CsA-treated turkeys showed delayed recovery from aMPV-induced clinical signs and histopathological lesions and a prolonged detection of aMPV in choanal swabs. The results of this study show that T-lymphocytes play an important role in the control of primary aMPV-infection in turkeys. Copyright (c) 2010 Elsevier Ltd. All rights reserved.

  20. Avian metapneumovirus RT-nested-PCR: a novel false positive reducing inactivated control virus with potential applications to other RNA viruses and real time methods.

    Science.gov (United States)

    Falchieri, Marco; Brown, Paul A; Catelli, Elena; Naylor, Clive J

    2012-12-01

    Using reverse genetics, an avian metapneumovirus (AMPV) was modified for use as a positive control for validating all stages of a popular established RT-nested PCR, used in the detection of the two major AMPV subtypes (A and B). Resultant amplicons were of increased size and clearly distinguishable from those arising from unmodified virus, thus allowing false positive bands, due to control virus contamination of test samples, to be identified readily. Absorption of the control virus onto filter paper and subsequent microwave irradiation removed all infectivity while its function as an efficient RT-nested-PCR template was unaffected. Identical amplicons were produced after storage for one year. The modified virus is likely to have application as an internal standard as well as in real time methods. Additions to AMPV of RNA from other RNA viruses, including hazardous examples such HIV and influenza, are likely to yield similar safe RT-PCR controls. Copyright © 2012 Elsevier B.V. All rights reserved.

  1. Reversion to virulence of a subtype B avian metapneumovirus vaccine: is it time for regulators to require availability of vaccine progenitors?

    Science.gov (United States)

    Cecchinato, M; Catelli, E; Lupini, C; Ricchizzi, E; Prosperi, S; Naylor, C J

    2014-08-06

    Empirically derived live avian metapneumovirus (AMPV) vaccines developed during the late 80s and early 90s have generally performed well in controlling turkey rhinotracheitis. Nonetheless, unstable attenuation was previously demonstrated in an AMPV subtype A vaccine. Until now this had not been investigated in subtype B vaccines due to lack of any similar availability of a vaccine progenitor or its sequence. The publication of the full genome sequence for the VCO3 vaccine progenitor facilitated a conclusive investigation of two AMPVs isolated from poults on a farm which had been vaccinated with VCO3 derived vaccine. Full genome sequencing of the isolates and their comparison to sequences of the vaccine and its progenitor, confirmed their vaccine origin. After determining the absence of extraneous infectious agents, one of these virus isolates was inoculated into 1-day-old turkeys in disease secure isolators and shown to cause disease with a severity similar to that caused by virulent field virus. This suggests that instability in live AMPV vaccines may be generalized and highlights the need for availability of vaccine progenitor sequences for the field assessment of all live viral vaccines. Copyright © 2014 Elsevier Ltd. All rights reserved.

  2. Compromised T-cell immunity in turkeys may lead to an unpredictable avian metapneumovirus vaccine response and variable protection against challenge.

    Science.gov (United States)

    Rubbenstroth, Dennis; Rautenschlein, Silke

    2010-10-01

    Avian metapneumovirus (aMPV) is an important respiratory pathogen of turkeys with considerable economic impact on poultry production. Although vaccination is widely used for the control of the disease, questions regarding vaccine safety and efficacy remain to be elucidated. This report describes the problems associated with reproducibility of the aMPV-vaccine response, comparing T-lymphocyte-compromised and T-cell-intact turkeys. In three consecutive experiments, turkeys partially depleted of T-lymphocytes by treatment with cyclosporin A as well as untreated turkeys were vaccinated with a commercial live aMPV subtype A (aMPV-A) vaccine at 2 weeks of age. Two weeks later they were challenged with a virulent aMPV-A strain. Despite similar genetic background of the turkeys, comparable housing conditions under isolation and the application of the same aMPV-A vaccine, considerable variation was observed among the experiments regarding replication of the vaccine virus, vaccine-induced clinical signs and protection against challenge infection. The results indicate that differences in the outcome of aMPV-A vaccination may be associated with T-lymphocyte suppression and additionally with an interfering aMPV-B vaccine exposure at the hatchery in two of the experiments. Our study provides possible explanations for the variable protection provided by aMPV vaccines under field conditions.

  3. Integrin αvβ1 Modulation Affects Subtype B Avian Metapneumovirus Fusion Protein-mediated Cell-Cell Fusion and Virus Infection.

    Science.gov (United States)

    Yun, Bing-Ling; Guan, Xiao-Lu; Liu, Yong-Zhen; Zhang, Yao; Wang, Yong-Qiang; Qi, Xiao-Le; Cui, Hong-Yu; Liu, Chang-Jun; Zhang, Yan-Ping; Gao, Hong-Lei; Gao, Li; Li, Kai; Gao, Yu-Long; Wang, Xiao-Mei

    2016-07-08

    Avian metapneumovirus (aMPV) fusion (F) protein mediates virus-cell membrane fusion to initiate viral infection, which requires F protein binding to its receptor(s) on the host cell surface. However, the receptor(s) for aMPV F protein is still not identified. All known subtype B aMPV (aMPV/B) F proteins contain a conserved Arg-Asp-Asp (RDD) motif, suggesting that the aMPV/B F protein may mediate membrane fusion via the binding of RDD to integrin. When blocked with integrin-specific peptides, aMPV/B F protein fusogenicity and viral replication were significantly reduced. Specifically we identified integrin αv and/or β1-mediated F protein fusogenicity and viral replication using antibody blocking, small interfering RNAs (siRNAs) knockdown, and overexpression. Additionally, overexpression of integrin αv and β1 in aMPV/B non-permissive cells conferred aMPV/B F protein binding and aMPV/B infection. When RDD was altered to RAE (Arg-Ala-Glu), aMPV/B F protein binding and fusogenic activity were profoundly impaired. These results suggest that integrin αvβ1 is a functional receptor for aMPV/B F protein-mediated membrane fusion and virus infection, which will provide new insights on the fusogenic mechanism and pathogenesis of aMPV. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

  4. Field estimation of the flock-level diagnostic specificity of an enzyme-linked immunosorbent assay for Avian metapneumovirus antibodies in turkeys.

    Science.gov (United States)

    Muñoz-Zanzi, Claudia; Trampel, Darrell; Hanson, Tim; Harrison, Kristen; Goyal, Sagar; Cortinas, Roberto; Lauer, Dale

    2009-03-01

    Routine serologic testing for Avian metapneumovirus (AMPV) infection of turkey flocks at slaughter is currently being used to monitor changes in the occurrence of AMPV infection in endemic areas and can also be used to detect the emergence of infection in currently unaffected areas. Because of the costs associated with false-positive results, particularly in areas that are free of AMPV infection, there is a need to obtain improved estimates of flock-level specificity (SP). The objective of this study was to estimate flock-level SP of a program to monitor AMPV infection in turkey flocks at processing using a standard enzyme-linked immunosorbent assay (ELISA). A study was carried out in which 37 AMPV-free flocks from 7 Midwest operations were followed serologically. Six percent, 3%, and 0.2% of total samples tested AMPV positive at 8 weeks, 12 weeks, and at processing, respectively. Overall, flock-level SP increased as the cutoff increased and as age increased. Flock-level SP at processing was 97%, if a cutoff of 1 was used (the flock was classified as positive if at least 1 sample tested positive), and 100%, if any other cutoff was used. Administration of antibiotics (P = 0.02) and vaccination for Bordetella avium (P = 0.08) were positively associated with the probability of (false) positive test results. These findings suggest possible cross-reactions with other infections and highlight the need to consider variable diagnostic performance depending on farm conditions.

  5. Role of trypsin in the replication of Avian metapneumovirus subtype C (strain MN-2a) and its entry into the Vero cells.

    Science.gov (United States)

    Paudel, Sarita; Shin, Hyun-Jin

    2015-12-01

    To understand the molecular mechanisms of Avian metapneumovirus (aMPV) and the requirements involved in the infection and fusion, trypsin treatment was done in the different stages of virus; before infection, during entry and after virus infection followed by aMPV infection. The growth kinetics of aMPV was compared in time dependent manner. The effect of trypsin was found in the later stage of aMPV infection increasing the numbers of infected cells with the significant higher titer of infectious virions to that of trypsin treated before infection, during entry and aMPV. A serine protease inhibitor reduced aMPV replication in a significant way, whereas cysteine peptidase (E-64), aspartic protease (pepstatin A), and metalloprotease (phosphoramidon) inhibitors had no effect on aMPV replication. Inoculation of aMPV on Vero cells expressing the membrane-associated protease TMPRSS2 resulted in higher virus titers than that inoculated on normal Vero cells and is statistically significant (p < 0.05). Also, an inhibitor of clathrin/caveolae-mediated endocytosis had no effect on virus progeny, indicating that aMPV does not use the endocytic pathway for entry but undergoes direct fusion. The effect of lysosomotropic agents was not significant, suggesting that aMPV does not require low-pH environment in endosomes to fuse its envelope with the plasma membrane. Copyright © 2015 Elsevier Ltd. All rights reserved.

  6. Integrin αvβ1 Modulation Affects Subtype B Avian Metapneumovirus Fusion Protein-mediated Cell-Cell Fusion and Virus Infection*

    Science.gov (United States)

    Yun, Bing-Ling; Guan, Xiao-Lu; Liu, Yong-Zhen; Zhang, Yao; Wang, Yong-Qiang; Qi, Xiao-Le; Cui, Hong-Yu; Liu, Chang-Jun; Zhang, Yan-Ping; Gao, Hong-Lei; Gao, Li; Li, Kai; Gao, Yu-Long; Wang, Xiao-Mei

    2016-01-01

    Avian metapneumovirus (aMPV) fusion (F) protein mediates virus-cell membrane fusion to initiate viral infection, which requires F protein binding to its receptor(s) on the host cell surface. However, the receptor(s) for aMPV F protein is still not identified. All known subtype B aMPV (aMPV/B) F proteins contain a conserved Arg-Asp-Asp (RDD) motif, suggesting that the aMPV/B F protein may mediate membrane fusion via the binding of RDD to integrin. When blocked with integrin-specific peptides, aMPV/B F protein fusogenicity and viral replication were significantly reduced. Specifically we identified integrin αv and/or β1-mediated F protein fusogenicity and viral replication using antibody blocking, small interfering RNAs (siRNAs) knockdown, and overexpression. Additionally, overexpression of integrin αv and β1 in aMPV/B non-permissive cells conferred aMPV/B F protein binding and aMPV/B infection. When RDD was altered to RAE (Arg-Ala-Glu), aMPV/B F protein binding and fusogenic activity were profoundly impaired. These results suggest that integrin αvβ1 is a functional receptor for aMPV/B F protein-mediated membrane fusion and virus infection, which will provide new insights on the fusogenic mechanism and pathogenesis of aMPV. PMID:27226547

  7. CONSIDERACIONES PARA LA PLANIFICACIÓN DE RECURSOS HUMANOS EN HOTELES DE LA PROVINCIA DE MANABÍ

    Directory of Open Access Journals (Sweden)

    Rodney Alfonso Alfonso

    2015-10-01

    Full Text Available El objetivo de esta investigación fue realizar consideraciones relacionadas con la actividad de planificación de recursos humanos para las entidades hoteleras de la provincia de Manabí. Se presentó la propuesta de una herramienta para el diagnóstico de la planificación de recursos humanos en entidades hoteleras, que tomó en cuenta la conformación de un equipo de trabajo especializado, una caracterización general del objeto de estudio y de manera específica o detallada del área de recursos humanos. Seguido a esto, se analiza la planificación de recursos humanos, tomando en cuenta factores internos y externos, basándose específicamente en un análisis DAFO ponderado con el criterio de expertos previamente seleccionados y la determinación del estado actual o nivel de desarrollo de la planificación de Recursos Humanos en los hoteles. Los resultados del presente trabajo han sido importantes para la planificación de recursos humanos de forma eficaz y operativa en los hoteles que integran el sistema turístico de Manabí. Además, las entidades hoteleras de la provincia demandan de una gestión de sus recursos humanos con enfoque integral y sistémico, donde es decisivo el papel que desempeña la Planificación de los recursos humanos, con una transformación tanto de métodos como de pensamiento, para emprender acciones en el logro de mejores resultados y no solo el empleo de la experiencia o intuición de los directivos.

  8. La dignidad del embrión humano. ¿Qué sentido tiene que se le niegue al embrión humano el derecho a vivir?

    OpenAIRE

    Juan de Dios Vial Correa; Angel Rodríguez Guerrero

    2009-01-01

    El concepto de “dignidad humana” ha ido evolucionando en la historia de la culturaoccidental hasta verla como algo intrínseco a la persona humana y no vinculado a una o más de las cualidades del ser humano con más o menos gradación; “todas las cosas tienen precio; pero el hombre tiene dignidad”. Más bien, es propio cuando el hombre pierde sus cualidades físicas, intelectuales, morales que resplandece más su pura dignidad. Por tanto, si el embrión es un ser humano (porque ha empezado un desarr...

  9. Entre la educación en derechos humanos y los derechos humanos escolares: una aproximación desde la perspectiva de los saberes escolares

    OpenAIRE

    Leidy Viviana Muñoz Hurtado; Douglas Giovany Rodríguez Heredia

    2016-01-01

    Comprender la insuficiencia de la educación en derechos humanos como herramienta analítica implica definir el campo simbólico que rodea, desde hace más de tres décadas, su institucionalización. Sin lugar a dudas, la principal razón para impugnar la idea de educación en derechos humanos como un saber escolar —sin que por ello no permita reflexionar acerca de diversas relaciones entre escuela y derechos humanos— tiene que ver con que no se produce en el marco de la escuela misma, e incluso, a d...

  10. Rorty, el inmanentismo absoluto y los derechos humanos

    Directory of Open Access Journals (Sweden)

    José Benjamín Rodríguez-Iturbe

    2012-01-01

    Full Text Available Richard Rorty es una de las figuras más destacadas del pensamiento filosófico- político norteamericano contemporáneo. Se le ubica en la tradición pragmática de William James y John Dewey. Su neopragmatismo forma parte de la filosofía analítica anglo norteamericana. Muestra una radical postura antimetafísica. Para él no tiene sentido hablar de problemas de la filosofía o problemas del lenguaje. No busca fundamentar nada y posee una visión sui generis del carácter democrático de la discusión pública. Proclama la sustitución de la fundamentación ética por aquellas que considera prácticas sociales de las sociedades democráticas. Solo considera sociedades democráticas aquellas regidas por un fundamentalismo secularista. En él, el conocimiento es sustituido por la conversación; y en la conversación el vocabulario es opcional y mudable. Los vocabularios obedecen a representaciones. Su neopragmatismo puede servir a concepciones políticas no liberales ni democráticas. Ello se muestra en su visión de los derechos humanos, reducidos a construcciones de lenguaje por rechazar toda referencia ontológica a la persona.

  11. DIREITOS HUMANOS: DIVERSIDADE CULTURAL, EDUCAÇÃO E DEMOCRACIA

    Directory of Open Access Journals (Sweden)

    José Wilson Rodrigues de Melo

    2016-06-01

    Full Text Available O objetivo deste artigo é apresentar uma conceituação ampla dos direitos humanos (DDHH, tendo como ponto fulcral a dignidade humana. O objeto do estudo é entrecortado pela tríade educação, diversidade cultural e democracia. Tomando-se a diferença como elemento de esteio das sociedades multiculturais, busca-se na educação um elemento fundamental para o respeito à diversidade na perspectiva da emancipação. Uma educação efetivada por meio de um currículo multicultural. Neste, a diferença é um atributo de respeito para a superação das desigualdades. Os racismos e preconceitos são instituídos como elementos danosos à democracia. É premente, pois, articular a igualdade e a diferença. Assim, a atenção à diferença institui-se como forma de reconhecimento e autonomia. O direito à diferença é indispensável à dignidade humana. Do contrário, os “humanos” perdem a completude. Respeitar as diferenças é uma busca constante de emancipação e estabelecimento de uma cultura de paz.

  12. Educação: necessidade corporativa e determinante humano

    Directory of Open Access Journals (Sweden)

    Antonio Mendes Silva Filho

    2014-04-01

    Full Text Available O pernambucano Paulo Freire ensinou que a “Educação não transforma o mundo. Educação muda pessoas. Pessoas transformam o mundo”. Educação é processo e ela é fundamental ao crescimento em todos os aspectos do ser humano. Para as organizações, o trabalho é considerado como uma base, um princípio educativo. Especificamente, a educação profissional visa proporcionar a compreensão das dinâmicas sócio-produtivas das sociedade moderna. No âmbito pessoal, a educação permite o indivíduo fazer escolhas adequadas. Essas escolhas podem ser equivocadas e pode exigir da pessoa retroceder, mudar o rumo, perdoar, mais treinamento ou tantas outras coisas a depender da situação que o indivíduo venha a se defrontar. Este artigo discute a importância da educação e sua possível influência sobre a natureza humana. O artigo também explora aspectos da natureza humana

  13. Dificultades actuales del sistema internacional de derechos humanos

    Directory of Open Access Journals (Sweden)

    José Augusto Lindgren-Alves

    2017-11-01

    Full Text Available Los derechos humanos, después de alzcanzar el punto culminante de popularidad en la Conferencia de Viena de 1993, entraron en uma fase de descrédito, que aún perdura. Eso ocurrió por varios motivos, algunos de los cuales están al interior del propio sistema estabelecido para promocionarlos. Los factores principales advienen de la contradicción entre la aserción verbal de los derechos fundamentales de todos y el “consenso” neoliberal simultaneamente impuesto a la esfera económica. Otros son inherentes al activismo fragmentario, sin noción de causa y efecto, desvinculado de la realidad en vuelta. Los problemas de segundo tipo son corregibles si para eso hubiese coherencia y destemor de críticas en las atitudes de los actores influyentes. El texto examina los componientes de ese sistema internacional e identifica las razones de su presente desvaluación.

  14. A soberania e os direitos humanos Sovereignty and human rigts

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    Celso Lafer

    1995-01-01

    Full Text Available À suposição hobbesiana de que não há limites éticos ao subjetivismo das soberanias, no plano internacional, podem se contrapor dois modelos, um inspirado em Grocio e o outro em Kant. Somente o modelo kantiano é capaz de reconhecer plenamente a universalidade dos direitos humanos e responder aos desafios que as tendências centrípedas (produzidas pela globalização econômica e centrífugas (originadas dos nacionalismos e particularismos étnicos e religiosos de todo tipo apresentam à convivência internacional.The Hobbesian claim that in international relations there is no way of submitting the subjectivism of the sovereingties to ethical constraints may be rejected on either Grotius' or Kant's terms. But only a broadly Kantian approach is able to recognize the universality of human rights and to meet two kinds of challenge to international coexistence: those related to the centripetal forces of global economic interdependence and those related to the centrifugal forces arising from the sharpening of national, ethnic and religious differences.

  15. humano. Un estudio en 3º de ESO

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    Jesús Ramón Girón Gambero

    2015-01-01

    alimentos. La investigación se ha llevado a cabo con el objetivo de estudiar el desempeño del alumnado en elanálisis de un video publicitario en un contexto real de aula, mediante un estudio de caso en 3º de EducaciónSecundaria Obligatoria. Se utiliza un anuncio televisivo que incluye un modelo para explicar cómo actúa unconocido producto probiótico en el organismo. Para obtener datos y evidencias se han utilizado el diario delprofesor, grabaciones en vídeo de las clases y los cuadernos de trabajo de los estudiantes. En los resultados sedestacan los elementos más fácilmente interpretables y las dificultades del alumnado para comprender el modelodel anuncio. Finalmente, como implicación educativa, se formulan algunas ideas que podrían formar parte de un modelo escolar que explique cómo actúan las defensas en el intestino humano, integrando aquellos aspectos positivos identificados en el anuncio con algunos elementos de los propios modelos de los alumnos y las ideas científicas al respecto.

  16. Estimación temporal en seres humanos mediante el procedimiento pico con interrupciones

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    Ramsés Vázquez-Lira

    2011-03-01

    Full Text Available Diversos procedimientos empleados en el estudio del aprendizaje animal hansido adaptados para investigación con humanos; específicamente, los procedimientosde intervalo fijo, bisección y generalización temporal, y el procedimientopico, originalmente empleados para estudiar el proceso de estimación temporalen animales no humanos, han sido también utilizados con humanos, brindandoimportantes conocimientos para la comprensión del comportamiento humano. Elpresente trabajo reporta la adaptación para humanos del procedimiento pico coninterrupciones, utilizado en las últimas décadas para dilucidar aspectos atencionalesy mnémicos del proceso de estimación temporal. Se encontró que los 35participantes (22.3 ± 3.1 años de edad tienden a detener su estimación temporaldurante la interrupción, conservando en memoria temporal el tiempo previo a lainterrupción; los presentes resultados sugieren que la tasa de decaimiento dememoria temporal en humanos es menor que en cualquiera de las otras especiesque han sido evaluadas en este procedimiento.

  17. Uma análise discursiva da abordagem nacional dos direitos humanos na imprensa escrita brasileira

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    Cássia Maria Rosato

    2017-01-01

    Full Text Available El presente artículo investiga cómo los Derechos Humanos fueron difundidos en la prensa escrita brasileña, a través del análisis de editoriales del periódico Folha de Sao Paulo en 1987 y 1997. Se trata de una investigación cualitativa con referencial teó- rico-metodológico de la Psicología Social Discursiva y de herramientas conceptuales de Michel Foucault. Los principales resultados revelan la existencia de una dicotomía en el modo como los Derechos Humanos se comprenden en los contextos nacionales e internacionales. En contextos nacionales, los Derechos Humanos suelen asociarse a la seguridad pública y el crimen. De otro lado, en coyunturas internacionales, el periódico propaga una noción más ampliada de Derechos Humanos, especialmente derechos civiles y políticos. La principal diferencia se refiere al énfasis dado a los Derechos Humanos como cuestión de prisiones, en la década de 1980, mientras que en la década de 1990, la policía surge como un importante actor en el campo de Derechos Humanos, especialmente en un enfoque de violación de derechos.

  18. CUADRO DE MANDO INTEGRAL PARA LA GESTIÓN DE LOS RECURSOS HUMANOS

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    Tamara Fernández Lima

    2008-05-01

    Full Text Available La gestión de los recursos humanos con un enfoque estratégico se ha convertido en una demanda del entorno empresarial. De ahí que el problema, para el gestor de los recursos humanos, sea encontrar el modo de vincular la gestión de los recursos humanos con la estrategia y la visión. Este artículo se propone realizar esta alineación utilizando el cuadro de mando integral como sistema de gestión estratégica. Se define un mapa genérico del cuadro de mando integral de la gestión estratégica de los recursos humanos que sirva de base para realizar el diagnóstico inicial de la GRH en una empresa. Como resultado se obtiene el cuadro de mando integral para la gestión de los recursos humanos, que permitirá a la gerencia técnica de SEPSA alinear los procesos claves de la GRH hacia el logro de la estrategia empresarial; con un sistema de objetivos, indicadores, metas e iniciativas para cada perspectiva que permita la implantación del mismo así como su revisión y adecuación constante. Palabras Clave Gestión de los recursos humanos

  19. O direito à terra como um direito humano: a luta pela reforma agrária e o movimento de direitos humanos no Brasil

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    Rossana Rocha Reis

    2012-01-01

    Full Text Available O objetivo deste texto é analisar, por meio da história recente dos movimentos sociais que lutam pela reforma agrária no Brasil, a construção do direito à terra como um direito humano. A partir dos anos de 1970, a interação de movimentos camponeses da Igreja Católica progressista e da rede transnacional de direitos humanos confluiu na produção dessa ideia da posse da terra como um direito humano, que marcou não apenas a luta pela terra no Brasil, como também influenciou a forma como o próprio movimento de direitos humanos foi construído no país e na maneira como ele se inseriu nessa rede transnacional de ativistas.This article seeks to analyze, within the recent history of social movements that struggle for agrarian reform in Brazil today, the build of the land rights as a human right. Since the 1970s, the interaction between peasant movements, the progressive church and the transnational network of human rights has converged into this idea that the land rights are a human right, which not only has marked the character of the struggle for land in Brazil, but has also influenced the way in which the human rights movement has been constructed in the country and how it has taken its place within the transnational network of human rights activism.

  20. Anarquismo español y derechos humanos

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    Jesús LÓPEZ SANTAMARÍA

    2011-04-01

    Full Text Available Si resulta evidente la proverbial aversión al Derecho por parte del mundo libertario, no lo es menos el complejo ejercicio que han desplegado el poder y la publicística no ácrata para construir el tópico del «anti-derecho» y contraponer, a la idea libertaria de una sociedad nueva, la imagen caótica de un sistema sin Derecho. Frente a ese perfil ideológico que define el Anarquismo como un movimiento alérgico a todo cuanto se refiera al campo del Derecho y también frente a esa construcción interesada del «anti-derecho», este trabajo pretende refutar ambos extremos recurriendo, por un lado, a la renovación teórica del Anarquismo y a la «puesta al día» del nuevo instrumental teórico que, a partir de finales de la década de los setenta del siglo pasado, convulsionó al colectivo ácrata; y, por otro, recuperando la producción textual orgánica y secuenciándola con el objetivo de evidenciar la recurrente intervención del colectivo anarquista español en el campo de la lucha por los derechos humanos. Circunstancia esta última que merece especial consideración, porque si desde el propio mundo anarquista se responde ante ella con dubitativa aceptación, no es de extrañar que no aparezca en el campo textual de la producción de los especialistas ajenos al Anarquismo.

  1. O ENCARCERAMENTO FEMININO À LUZ DOS DIREITOS HUMANOS

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    Cleide Leite de Sousa dos Anjos

    2016-11-01

    Full Text Available No escopo deste artigo sobre o encarceramento feminino à luz dos direitos humanos brasileiro, de acordo com as estatísticas da Infopen, de 2014, o Brasil contou com uma população de 579.7811 pessoas custodiadas no Sistema Penitenciário Brasileiro, sendo 37.380 mulheres e 542.401 homens. Ao longo deste trabalho, ficou claro que a transgressão à lei confere ao Estado o direito de punir, e que esta é aplicada de acordo com as normas jurídicas vigentes, com a diminuição ou perda dos bens jurídicos, sendo ao réu aplicados os efeitos de uma sentença e, ao mesmo passo, uma obrigação, devendo submeter-se. Assim, tem-se que a pena é vista como uma forma de defender a sociedade que se encontra organizada juridicamente contra os perigos ou ameaças por parte do delinquente, neste caso específico as presas mulheres. Embora muitos preguem a necessidade de maior rigidez do ordenamento jurídico, especialmente no que se refere à aplicação de leis mais severas, tanto para a aplicação da pena, quanto na execução desta. Todavia, não há dúvida de que a legislação penal pátria é bastante severa, porém necessita de que a sua aplicação e acompanhamento se deem de maneira correta por parte do Poder Público. A metodologia utilizada foi o método bibliográfico.

  2. Una concepción integradora del aprendizaje humano

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    Diego González Serra

    2015-06-01

    Full Text Available http://dx.doi.org/10.5007/2175-795X.2015v33n1p119 En el decurso histórico de la psicología científi ca han sido desarrolladas diferentes teorías del aprendizaje que son unilaterales pues abordan aspectos parciales, los cuales son concebidos de manera absoluta en detrimento de la consideración de otros. Sirva de ejemplo la contraposición entre las teorías de estímulo-respuesta, de un lado, y las teorías cognitivas o humanistas del otro. Por ello, los que somos partidarios del conocimiento más pleno y objetivo del psiquismo, consideramos necesario trabajar en pro de una teoría dialéctica, sintética o integradora, del aprendizaje que asimile críticamente, vuelva a elaborar e integre todo lo que la psicología mundial ha aportado en esta dirección. De acuerdo con el principio de la unidad dialéctica del análisis y la síntesis, esto no niega sino afi rma la necesidad de teorías analíticas o parciales que profundicen, sin hiperbolizar ni caer en enfoques unilaterales, en la naturaleza de los distintos aspectos y momentos del aprendizaje humano y animal. Nuestra posición es dialéctica por cuanto nos esforzamos por un enfoque multilateral que enfatice el aprendizaje humano como un refl ejo de su medio socio histórico y a la vez tenga en cuenta su función creadora de aquello que se aprende. Planteamos la necesidad de desarrollar y exponer una teoría psicológica del aprendizaje como una temática central de la psicología general y de la pedagógica, de gran importancia teórica y práctica. Esta es una tarea colectiva y a largo plazo. En el presente capítulo avanzamos algunos criterios que podemos discutir, mantener, abandonar o perfeccionar.   Uma visão integradora da aprendizagem humana  Resumo No percurso histórico da psicologia científica foram desenvolvidas diferentes teorias de aprendizagem que, por serem unilaterais, abordam aspectos parciais. Ao concebê-las de maneira absoluta, ignoram-se outros

  3. RECURSOS HUMANOS EN LAS FILOSOFÍAS GERENCIALES Y TENDENCIAS DE LA GESTIÓN DE LOS RECURSOS HUMANOS EN EL MUNDO

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    María Sonia Fleitas Triana

    2002-10-01

    Full Text Available

    El mundo empresarial contemporáneo está caracterizado por tres fuerzas que son determinantes en la definición de las estrategias empresariales: los clientes, la competencia y el cambio. Los recursos humanos constituirán en el siglo XXI el recurso competitivo más importante, pues son determinantes en la materialización de las relaciones con los clientes, constituyen una fuente de ventaja competitiva sustentable y con una adecuada preparación desempeñan un importante papel como agentes de cambio de las organizaciones. Sin embargo, todo lo anterior solo es posible cuando se logra una correcta gestión de dichos recursos. En el articulo se expondrán antecedentes de la actual gestión de recursos humanos, la dimensión humana de la empresa del futuro y los rasgos más significativos de las actuales concepciones de gestión de los recursos humanos.Se presentan modelos de gestión de recursos humanos reconocidos internacionalmente insistiendo en el carácter sistemático de la gestión de los recursos humanos y en la definición de políticas y actividades claves que deben ejecutar los empresarios con vistas a lograr bienestar individual, bienestar social y eficacia en la organización.

  4. La implementación de los Principios Rectores ONU sobre empresas y derechos humanos en España

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    Inmaculada Vivas-Tesón

    2013-12-01

    Full Text Available En los últimos años se ha cristalizado la expectativa social de que los entes empresariales, y en particular, las empresas transnacionales, tienen el deber de respetar los derechos humanos. Esta expectativa se ha plasmado, entre otros, en la adopción en 2011 de los Principios Rectores sobre empresas y derechos humanos por el Consejo de Derechos Humanos de Naciones Unidas. En esta comunicación trataremos las implicaciones para España del marco de Naciones Unidas sobre empresas y derechos humanos y sus implicaciones para España.

  5. Influencia del capital humano en la calidad de la auditoría contable

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    Mónica Fernández

    2013-12-01

    Full Text Available Objeto: Se estudian los determinantes de calidad del capital humano y del servicio en empresas de auditoría y principalmente cómo cualidades del capital humano inciden en la calidad del servicio prestado. Diseño/metodología: Se realizó un cuestionario a una muestra de socios de una firma de auditoría relevante. Con los datos de los cuestionarios se obtuvieron los mejores indicadores de calidad de capital humano y calidad del servicio respectivamente. Posteriormente, se elaboran tablas de contingencia, con los datos tratados, para determinar las probabilidades de encontrar equipos con elevado nivel de capital humano, equipos con elevado nivel de servicio y ver si dentro de los equipos con alto desarrollo de su personal, el nivel del servicio es más alto o no. Aportaciones y resultados: La calidad del factor humano es un activo intangible determinante en las organizaciones, especialmente en las que prestan servicios que requieren una alta capacitación profesional como ocurre en el sector de auditoría. Los resultados ponen de manifiesto que determinadas capacidades del capital humano inciden de forma relevante en factores asociados a la calidad del servicio de auditoría. El análisis realizado sugiere que una buena gestión e inversión en el capital humano de las mencionadas empresas, incide en la calidad del servicio prestado al cliente. Limitaciones: El trabajo se ha podido realizar en una única firma de auditoría y la muestra no ha sido muy elevada. Pretendemos conseguir ampliar las muestras y las firmas participantes en próximas investigaciones. Valor añadido: En este análisis la variable más destacada, en cuanto a capital humano se refiere, es la experiencia y formación en la industria. Políticas de buena gestión del capital humano pueden fomentar una cultura donde el objetivo de ética y transparencia, contribuyan a mejorar los servicios prestados.

  6. Generation and evaluation of a recombinant Newcastle disease virus expressing the glycoprotein (G) of avian metapneumovirus subgroup C as a bivalent vaccine in turkeys.

    Science.gov (United States)

    Hu, Haixia; Roth, Jason P; Estevez, Carlos N; Zsak, Laszlo; Liu, Bo; Yu, Qingzhong

    2011-11-03

    Virulent strains of Newcastle disease virus (NDV) and avian metapneumovirus (aMPV) can cause serious respiratory diseases in poultry. Vaccination combined with strict biosecurity practices has been the recommendation for controlling both NDV and aMPV diseases in the field. In the present study, an NDV based, LaSota strain recombinant vaccine virus expressing the glycoprotein (G) of aMPV subgroup C (aMPV-C) was generated as a bivalent vaccine using a reverse genetics approach. The recombinant virus, rLS/aMPV-C G was slightly attenuated in vivo, yet maintained similar growth dynamics, cytopathic effects, and virus titers in vitro when compared to the parental LaSota virus. Expression of the aMPV G protein in rLS/aMPV-C G-infected cells was detected by immunofluorescence assay. Vaccination of turkeys with one dose of rLS/aMPV-C G induced moderate aMPV-C-specific immune responses and comparable NDV-specific serum antibody responses to a LaSota vaccination control. Partial protection against pathogenic aMPV-C challenge and complete protection against velogenic NDV challenge was conferred. These results suggest that the LaSota recombinant virus is a safe and effective vaccine vector and that expression of the aMPV-C G protein alone is not sufficient to provide full protection against an aMPV-C infection. Expression of other immunogenic protein(s) of the aMPV-C virus alone or in conjunction with the G protein may be needed to induce a stronger protective immunity against the aMPV-C disease. Published by Elsevier Ltd.

  7. Development of a real-time RT-PCR assay for the simultaneous identification, quantitation and differentiation of avian metapneumovirus subtypes A and B.

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    Cecchinato, Mattia; Lupini, Caterina; Munoz Pogoreltseva, Olga Svetlana; Listorti, Valeria; Mondin, Alessandra; Drigo, Michele; Catelli, Elena

    2013-01-01

    In recent years, special attention has been paid to real-time polymerase chain reaction (PCR) for avian metapneumovirus (AMPV) diagnosis, due to its numerous advantages over classical PCR. A new multiplex quantitative real-time reverse transcription-PCR (qRT-PCR) with molecular beacon probe assay, designed to target the SH gene, was developed. The test was evaluated in terms of specificity, sensitivity and repeatability, and compared with conventional RT nested-PCR based on the G gene. All of the AMPV subtype A and B strains tested were amplified and specifically detected while no amplification occurred with other non-target bird respiratory pathogens. The detection limit of the assay was 10(-0.41) median infectious dose/ml and 10(1.15) median infectious dose/ml when the AMPV-B strain IT/Ty/B/Vr240/87 and the AMPV-A strain IT/Ty/A/259-01/03 were used, respectively, as templates. In all cases, the amplification efficiency was approximately 2 and the error values were 0.9375) between crossing point values and virus quantities, making the assay herein designed reliable for quantification. When the newly developed qRT-PCR was compared with a conventional RT nested-PCR, it showed greater sensitivity with RNA extracted from both positive controls and from experimentally infected birds. This assay can be effectively used for the detection, identification, differentiation and quantitation of AMPV subtype A or subtype B to assist in disease diagnosis and to carry out rapid surveillance with high levels of sensitivity and specificity.

  8. Italian field survey reveals a high diffusion of avian metapneumovirus subtype B in layers and weaknesses in the vaccination strategy applied.

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    Cecchinato, Mattia; Lupini, Caterina; Ricchizzi, Enrico; Falchieri, Marco; Meini, Amelio; Jones, Richard C; Catelli, Elena

    2012-12-01

    The current information on the prevalence of avian metapneumovirus (aMPV) infection in layers is fragmentary and its true impact on egg production often remains unknown or unclear. In order to draw an epidemiologic picture of aMPV presence in layer flocks in Italy, a survey was performed on 19 flocks of pullets and layers based on longitudinal studies or sporadic samplings. aMPV was detected by reverse transcription (RT)-PCR, and blood samples were collected for serology by aMPV ELISA. Occurrences of respiratory signs and a drop in egg production were recorded. Possible involvement of infectious bronchitis (IB) and egg drop syndrome (EDS) viruses that could have caused loss of egg production we ruled out for IB virus by RT-PCR, and EDS virus was ruled out by hemagglutination-inhibition (HI). Only subtype B of aMPV was found in both pullet and layer farms. Surveys of pullets showed that most groups became infected prior to the onset of lay without showing clear respiratory signs. At the point of lay, these groups were serologically positive to aMPV. In two layer flocks, egg drops were observed and could be strongly linked to the presence of aMPV infection. Results were correlated with aMPV vaccination programs applied to the birds in three flocks on the same farm. Only a vaccination program which included two live and one killed vaccines gave complete protection from aMPV infection to the birds, while a single live vaccine application was not efficacious. The current study gives an inside view of field aMPV diffusion in Italy and its control in layers.

  9. Reproducibility of swollen sinuses in broilers by experimental infection with avian metapneumovirus subtypes A and B of turkey origin and their comparative pathogenesis.

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    Aung, Ye Htut; Liman, Martin; Neumann, Ulrich; Rautenschlein, Silke

    2008-02-01

    Swollen head syndrome (SHS) associated with avian metapneumovirus (aMPV) subtype A or subtype B in broilers and broiler breeders has been reported worldwide. Data about pathogenesis of aMPV subtypes A and B in broilers are scarce. It has been difficult to reproduce swollen sinuses in chickens with aMPV under experimental conditions. In the field, SHS in broilers is suspected to be induced by combined infections with different respiratory pathogens. The objectives of the present study were to compare the pathogenesis of subtypes A and B aMPV in commercial broilers and to investigate the reproducibility of clinical disease. In two repeat experiments, commercial broilers free of aMPV maternal antibodies were inoculated with aMPV subtypes A and B of turkey origin. The clinical signs such as depression, coughing, nasal exudates, and frothy eyes appeared at 4 days post inoculation, followed by swelling of periorbital sinuses at 5 days post inoculation. Higher numbers of broilers showed clinical signs in subtype-B-inoculated compared with subtype-A-inoculated groups. Seroconversion to aMPV was detectable from 10 to 11 days post inoculation. The appearance of serum aMPV enzyme-linked immunosorbent assay antibodies and the clearance of the aMPV genome coincided. Subtype B aMPV showed a broader tissue distribution and longer persistence than subtype A. Histopathological changes were observed in the respiratory tract tissues of aMPV-inoculated broilers, and also in paraocular glands, such as the Harderian and lachrymal glands. Overall, our study shows that representative strains of both aMPV turkey isolates induced lesions in the respiratory tract, accompanied by swelling of infraorbital sinuses, indicating the role of aMPV as a primary pathogen for broilers.

  10. Generation and biological assessment of recombinant avian metapneumovirus subgroup C (aMPV-C) viruses containing different length of the G gene.

    Science.gov (United States)

    Yu, Qingzhong; Estevez, Carlos; Song, Minxun; Kapczynski, Darrell; Zsak, Laszlo

    2010-02-01

    Genetic variation in length of the G gene among different avian metapneumovirus subgroup C (aMPV-C) isolates has been reported. However, its biological significance in virus replication, pathogenicity and immunity is unknown. In this study, we developed a reverse genetics system for aMPV-C and generated two Colorado (CO) strain-based recombinant viruses containing either the full-length G gene derived from a Canadian goose isolate or a C-terminally truncated G gene of the CO strain. The truncated short G (sG) gene encoded 252 amino acids (aa), which is 333 aa shorter than the full-length G (585 aa). The biological properties of these two recombinant G variants were assessed in Vero cells and in specific-pathogen-free (SPF) turkeys. In Vero cells, the short G variant displayed a similar level of growth dynamics and virus titers as the parental aMPV-CO strain, whereas the full-length G variant replicated less efficiently than the sG variant during the first 72 h post-infection. Both of the G variants induced typical cytopathic effects (CPE) that were indistinguishable from those seen with the parental aMPV-CO infection. In SPF turkeys, both of the G variants were attenuated and caused little or no disease signs, but the full-length G variant appeared to grow more readily in tracheal tissue than the sG variant during the first 5 days post-infection. Both G variants were immunogenic and induced a slightly different level of antibody response. These results demonstrated that the large portion (333 aa) of the extracellular domain of the viral attachment protein is not essential for virus viability in vitro and in vivo, but may play a role in enhancing virus attachment specificity and immunity in a natural host. (c) 2009 Elsevier B.V. All rights reserved.

  11. Protection conferred by a live avian metapneumovirus vaccine when co-administered with live La Sota Newcastle disease vaccine in chicks

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    Kannan Ganapathy

    2014-06-01

    Full Text Available This paper examines the effects on specific pathogen-free (SPF chicks when avian metapneumovirus (aMPV and Newcastle disease virus (NDV La Sota strain vaccines are co-administered. Day-old SPF chicks were divided into five groups. The first group was inoculated with sterile water (SW and the rest of the groups were inoculated with live NDV vaccine VG/GA by the oculo-oral route. At 21 days-old, the unvaccinated chicks were again inoculated with SW. The four VG/GA-vaccinated groups were further inoculated with (i SW, (ii live aMPV vaccine, (iii live NDV La Sota, or (iv combined live NDV La Sota and live aMPV, respectively. Chicks were monitored for post-vaccination reactions and oropharyngeal swabs were collected for vaccines detection. Blood samples were collected to detect aMPV ELISA and NDV haemagglutination-inhibition antibodies. Twenty-one days following the second vaccination, six chicks from each group were challenged with virulent NDV or aMPV respectively. Chicks were monitored for clinical signs and mortality and oropharyngeal swabs collected for aMPV detection. Results showed that, when challenged with a virulent aMPV, both chicks previously vaccinated with VG/GA and subsequently given aMPV vaccine singly or in combination with La Sota were equally protected against clinical signs. Chicks that were vaccinated against NDV either once with VG/GA or followed by La Sota (singly or in combination with aMPV were fully protected when challenged with velogenic NDV. We concluded that simultaneous administration of live aMPV and NDV La Sota vaccines have no adverse effects on protection conferred by either live vaccine.

  12. Pathogenesis of Riemerella anatipestifer in turkeys after experimental mono-infection via respiratory routes or dual infection together with the avian metapneumovirus.

    Science.gov (United States)

    Rubbenstroth, Dennis; Ryll, Martin; Behr, Klaus-Peter; Rautenschlein, Silke

    2009-12-01

    Riemerella anatipestifer (RA) is the causative agent of septicaemic and exudative diseases in a variety of bird species. Despite numerous outbreaks, little is known about the pathogenicity of RA for turkeys. We investigated the development of RA-induced disease in commercial turkey poults following RA inoculation via different respiratory routes. Inoculation by aerosol or injection into the abdominal air sac led to systemic infection and mild gross lesions, including pericarditis, epicarditis and airsacculitis, which were less pronounced compared with field outbreaks. It was speculated, that viral pathogens, such as the avian metapneumovirus (aMPV), may exacerbate RA pathogenesis under field conditions. We inoculated turkey poults with virulent aMPV. Subsequently, aMPV-infected and virus-free birds were exposed 3 to 5 days later to a high dose of RA by aerosol (>10(10) colony-forming units/ml in 8 ml aerosol per 11 or 12 birds) or were inoculated 4 days later with a low RA dose (10(4.9) colony-forming units per bird) via the intranasal route. Intranasal RA inoculation with the low bacterial dose led to a respiratory and systemic RA infection in aMPV-infected birds, while virus-free birds remained RA-negative. Following exposure to a high RA dose by aerosol, aMPV-infected groups showed slightly enhanced incidences of gross lesions and RA re-isolation. The present study clearly confirms that RA is pathogenic for turkeys after experimental inoculation via respiratory routes, which are speculated to be the natural route of infection. However, experimental models in this study did not reproduce the severity of RA-related disease as observed under field conditions, which emphasizes the importance of other contributing factors. aMPV-induced respiratory lesions may serve as a predisposing factor for the establishment of RA infection, since they favour colonization of the bacterium.

  13. A genetically engineered prime-boost vaccination strategy for oculonasal delivery with poly(D,L-lactic-co-glycolic acid) microparticles against infection of turkeys with avian Metapneumovirus.

    Science.gov (United States)

    Liman, Martin; Peiser, Lieselotte; Zimmer, Gert; Pröpsting, Marcus; Naim, Hassan Y; Rautenschlein, Silke

    2007-11-14

    In this study we demonstrated the use of an oculonasally delivered poly(D,L-lactic-co-glycolic acid) microparticle (PLGA-MP)-based and genetically engineered vaccination strategy in the avian system. An avian Metapneumovirus (aMPV) fusion (F) protein-encoding plasmid vaccine and the corresponding recombinant protein vaccine were produced and bound to or encapsulated by PLGA-MP, respectively. The PLGA-MP as the controlled release system was shown in vitro to not induce any cytopathic effects and to efficiently deliver the F protein-based aMPV-vaccines to avian cells for further processing. Vaccination of turkeys was carried out by priming with an MP-bound F protein-encoding plasmid vaccine and a booster-vaccination with an MP-encapsulated recombinant F protein. Besides the prime-boost F-specific vaccinated birds, negative control birds inoculated with a mock-MP prime-boost regimen as well as non-vaccinated birds and live vaccinated positive control birds were included in the study. The MP-based immunization of turkeys via the oculonasal route induced systemic humoral immune reactions as well as local and systemic cellular immune reactions, and had no adverse effects on the upper respiratory tract. The F protein-specific prime-boost strategy induced partial protection. After challenge the F protein-specific MP-vaccinated birds showed less clinical signs and histopathological lesions than control birds of mock MP-vaccinated and non-vaccinated groups did. The vaccination improved viral clearance and induced accumulation of local and systemic CD4+ T cells when compared to the mock MP-vaccination. It also induced systemic aMPV-neutralizing antibodies. The comparison of mock- and F protein-specific MP-vaccinated birds to non-vaccinated control birds suggests that aMPV-specific effects as well as adjuvant effects mediated by MP may have contributed to the overall protective effect.

  14. La protección de los derechos humanos en las Américas

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    Lorena Rincón Eizaga

    2004-01-01

    Full Text Available El artículo se centra en el estudio de la protección de los derechos humanos en el sistema interamericano, creado por los Estados de este continente en el marco de la Organización de Estados Americanos (OEA tomando como referente uno de sus principios esenciales: el respeto y garantía de los derechos de la persona humana. Se estudian los instrumentos interamericanos generales y constitucionales del sistema: la Declaración Americana de los Derechos y Deberes del Hombre (1948 y la Convención Americana sobre Derechos Humanos (1969, considerando los órganos principales encargados del control y tutela de las obligaciones estatales de respeto y garantía de los derechos reconocidos en dichos instrumentos: la Comisión y la Corte Interamericanas de Derechos Humanos. Se concluye que si bien constituye una conquista significativa el reconocimiento internacional de los derechos humanos, queda aún mucho camino por recorrer para lograr la efectiva protección de los mismos en el orden internacional, particularmente en el ámbito del sistema interamericano donde se advierten deficiencias políticas, estructurales y de procedimiento que dificultan el perfeccionamiento de su labor en una región donde persiste una situación generalizada de violación de los derechos humanos, con énfasis en los derechos económicos, sociales y culturales de la población latinoamericana.

  15. Los derechos humanos, una responsabilidad de la Psicología Jurídica

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    Gerardo A. Hernández M.

    2010-01-01

    Full Text Available El presente artículo tiene el propósito de llamar la atención de los psicólogos jurídicos en torno a lo que se conoce como los derechos humanos, pilar fundamental de la vida en sociedad. Para ello se hace una descripción de lo que son los derechos humanos y su respaldo legal, diferenciándolos de los derechos fundamentales. Por otro lado, se señala la importancia del conocimiento explícito de los derechos humanos por parte del psicólogo jurídico y la forma como los debe articular a su actividad profesional. Por lo anterior, este es un trabajo sui generis, al decir de los abogados, en tanto que no es el resultado de una investigación teórica sobre el tema de los derechos humanos, como tampoco hace parte de un trabajo empírico. La pretensión básica es que los psicólogos jurídicos conozcan el concepto de derechos humanos tal como está establecido en la norma internacional y su diferencia con los derechos fundamentales.

  16. Las 47 preguntas sobre el virus del papiloma humano, VPH

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    Elena de la Fuente Díez

    2008-09-01

    Full Text Available La prevención del cáncer de cuello uterino ha evolucionado rápidamente a consecuencia de la identificación de su causa, el Virus del Papiloma Humano (VPH. La asociación VPH/Cáncer de Cuello Uterino, es aplicable tanto al carcinoma escamocelular como al adenocarcinoma. Dos de los tipos oncogénicos, los VPHs 16 y 18, son responsables del 70% de los casos de cáncer de cuello uterino a nivel mundial. El VPH es muy prevalente entre personas sexualmente activas, y se puede identificar fácilmente mediante el uso de tecnologías capaces de detectar ADN y otros biomarcadores del VPH. El ADN y los biomarcadores se pueden detectar en todas las fases de la historia natural de la infección y del proceso neoplásico. Dicha trazabilidad unida a la validación clínica de las tecnologías de identificación, han permitido mejorar los protocolos de cribado del cáncer cervical. La proteína principal del virión, ya expresada, produce inmunógenos capaces de inducir fuertes respuestas inmunitarias cuando se administran vía intramuscular, y ésta es la base de la Vacuna frente a VPH.The UCC (Uterine Cervical Cancer prevention has suffered a great evolution because of the knowing of its cause, wich is the HPV (Human Papillomavirus. The association HPV / UCC is available also to Scamocellular Carcinome to Adenocarcinome. Mundially, the oncogenic types, 16 and 18, are responssible of 70% of cases of UCC. HPV is very prevalent in sexually active people, and it’s easy identify by using DNA amplyfiyng technologies and other biomarkers. These ones can be detected in hole virus natural life, including the neoplasic process. The tazability and the clinic validation of this technologies, have improved to get a better screening of the UCC. The main HPV protein, is able to produce a strong inmunitary response, when it’s given by intramuscular punctury; and this is the explanation of the HPC vaccine.

  17. ¿Ante, de, en, y? Mujeres, derechos humanos

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    Elizabeth JELIN

    2009-11-01

    Full Text Available RESUMEN: El trabajo integra dos planos: teórico y analítico, que confluyen en una explicación integral acerca de la condición de las mujeres, en perspectiva de lucha, democracia y desafíos. El primer plano es un recorrido por las concepciones paradigmáticas de los derechos humanos. Aquí es donde se plantea claramente la tensión entre universalismo y diferencias, la cual no puede ser resuelta sin atención a la «contextualización de los derechos en el sistema de relaciones sociales». El segundo nivel es el análisis de las luchas, logros y derechos de las mujeres latinoamericanas hasta nuestros días, con especial énfasis en el estado de cosas actual. En este último sentido, se retoman algunos temas concretos: «el derecho al propio cuerpo: los derechos reproductivos»; «la lucha por la igualdad: hacia la eliminación de todas las formas de discriminación»; «los derechos globales: paz, desarrollo y medio ambiente».ABSTRACT: The work integrates two levels: theoretic and analytic, that converge on an integral explanation about women's condition, in perspective of fight, democracy and challenges. First plane is a run across the paradigmatic conceptions of human rights. Here is when the tension between universalism and differences is stated, which can't be solved without paying attention «to putting rights in the context of the social relations system». Second level is the analysis of the fights, achievements and rights of latinamerican women until nowadays, with special emphasis on the state of things in present time. In this last sense, some concrete subjects are retaken: «the right to the self body: reproductive rights»; «the fight for equality: towards the elimination of all forms of discrimination»; «the global rights: peace, development, environment».

  18. Implicancias éticas del proyecto genoma humano

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    Maria Graciela De Ortuzar

    2014-06-01

    Full Text Available En el presente informe analizo críticamente el posible uso de la información genética individual y sus implicancias en la distribución de recursos en salud. Para ello, comienzo con el argumento de la ?equidad actuarial? de los seguros privados de salud, ficción histórica y estructural. Si bien considero que debe apoyarse la moratoria o prohibición del acceso a la información genética para los seguros privados -en razón de que el determinismo genético imperante profundizará aún más las desigualdades que se encuentran en su estructura-; dicha moratoria no constituye la solución definitiva al problema de fondo. Es preciso reconocer que la salud/enfermedad, en tanto definamos a la misma de forma integral y no unicausal, es el bien que debe protegerse (no discriminación por razones de salud, no pudiéndose reducirse la persona a sus genes. Los seguros de salud no pueden compararse con cualquier otra empresa comercial, debido a que su accionar tiene consecuencias políticas y sociales, impidiendo el desarrollo del plan de vida y la participación política. Por lo tanto, constituye una responsabilidad social el acceso universal a la salud, compartiendo riesgos. Y esto implica ampliar el principio de no discriminación existente en la legislación internacional2 con el fin de que el mismo no se base exclusivamente en la eliminación de barreras legales (derechos civiles y políticos, informales (sexo, raza, clase, religión, etc o genéticas (patrimonio genético; sino en una concepción de fuerte igualdad de oportunidades que garantice el cumplimiento de los derechos sociales y humanos, como lo son el derecho al acceso a la salud y a la tecnología genética.

  19. Aproximaciones sobre el derecho al trabajo desde la perspectiva de los Derechos Humanos

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    Ángela Molina-Higuera

    2010-03-01

    Full Text Available El derecho al trabajo es un derecho humano consagrado, tanto en su dimensión individual como colectiva, en múltiples instrumentos del sistema internacional de los derechos humanos. La Constitución Política colombiana de 1991 reconoce también el trabajo como principio, como deber y como derecho. A partir del sistema normativo internacional y nacional constitucional se presenta un concepto de trabajo que, desde una perspectiva de derechos humanos, debe ser digno. En esta dirección, con base en lo señalado por el Comité de Derechos Económicos, Sociales y Culturales en la Observación General No.18 sobre el derecho al trabajo, se identifica el contenido básico de este derecho y se refieren algunas obligaciones jurídicas del Estado para su efectiva realización.

  20. La pena de muerte desde la bioética y los derechos humanos

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    Octavio Márquez Mendoza

    2015-01-01

    Full Text Available Se ofrece una reflexión de la pena de muerte, que parte de premisas esenciales desde la bioética y los derechos humanos. Se plantean algunas generalidades de esta disciplina, dado que orientan a un cuestionamiento respecto al aporte de la ciencia y la tecnología para llevar a cabo “ejecuciones más humanas”. Enseguida se recurre a los conceptos de vida, persona y dignidad para enmarcar el argumento en los derechos humanos. Se retoman algunas sentencias de documentos internacionales apelando a un desarrollo del hombre desde la ética: la práctica de la pena máxima, por el contrario, lo retiene. Así pues, se anticipa que lo humano nunca se hallará en la aniquilación de su propio género.

  1. Derechos humanos y desarrollo económico: ¿cómo armonizarlos?

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    María Clara Galvis

    2013-06-01

    Full Text Available Este artículo aborda algunas de las tensiones entre el modelo de desarrollo económico que legítimamente promueven los Estados y el respeto de los derechos humanos en América Latina. Para ello se refiere al rol que el sistema interamericano de protección de derechos humanos ha tenido en este campo y a la forma en que ha enfrentado estas tensiones, en el marco de sus competencias. En particular, se revisan los principales casos en donde la Comisión Interamericana de Derechos Humanos ha otorgado medidas cautelares para proteger los derechos de miembros de comunidades indígenas y/o campesinas frente a los efectos de proyectos de infraestructura energética o de actividades extractivas.

  2. El estudio del factor humano en accidentes de aviación

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    Lina María Sánchez Rubio

    2010-01-01

    Full Text Available El factor humano ha sido considerado causa o contribución del 70 al 80% de los accidentes de aviación en el mundo, y constituye una prioridad en investigación para la seguridad aérea. Las estadísticas muestran que los índices y causas de accidentes de aviación se mantienen constantes, e identifican una importante diferencia entre regiones. La investigación científica requiere de nuevas y complementarias aproximaciones para la explicación y comprensión del factor humano en aviación. Los médicos y psicólogos que trabajan en Medicina Aeroespacial y factores humanos desempeñan un importante papel para alcanzar las metas en seguridad aérea.

  3. Educación en Derechos Humanos y Educación Superior: una perspectiva controversial

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    Abraham Magendzo Kolstrein

    2015-01-01

    Full Text Available Este artículo, a partir de una postura crítica, muestra la importancia de introducir y reforzar —como desafío ético y político— la educación en derechos humanos en la educación superior. Para formar profesionales que contribuyan, desde sus campos de acción, a crear una cultura ciudadana y respetuosa de los derechos humanos. La sugerencia es incorporar los derechos humanos en el currículum, la pedagogía, la didáctica y la evaluación en una perspectiva controversial, que permita profundizar en las tensiones que tienen los derechos para de esta manera, formar sujetos dialogantes, empoderados y transformadores de la sociedad.

  4. A pesquisa sobre envelhecimento humano no Brasil: grupos e linhas de pesquisa

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    Shirley Donizete Prado

    2004-01-01

    Full Text Available Descrevemos a pesquisa científica sobre envelhecimento humano no Brasil a partir da versão 4.1 do Diretório dos Grupos de Pesquisa no Brasil (2000 do Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq. O critério para a identificação dos grupos estudados foi o desenvolvimento de, pelo menos, uma linha de pesquisa referente ao envelhecimento humano, e a busca na base de dados foi realizada a partir de palavras-chave associadas ao envelhecimento humano. Foram identificados 144 grupos, 209 linhas de pesquisa e 511 pesquisadores. Discutimos as áreas de conhecimento em que se inserem estes grupos, o período de seu surgimento e sua distribuição geográfica e institucional.

  5. Teoria do Capital Humano: Breve Discussão Teórica

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    Rosângela Saldanha Pereira

    2011-04-01

    Full Text Available Neste texto exploramos os fundamentos teóricos da teoria do capital humano, a sua crítica aos pressupostos da teoria clássica e concluímos que a teoria do capital humano é um desdobramento singular dos postulados da teoria econômica marginalista aplicados à educação. Não objetivamos fazer um tratado sobre as teorias aqui abordadas. Estamos buscando apenas apresentar os vínculos do capital humano com a visão marginalista e destacar o avanço proporcionado por esta abordagem para a compreensão dos fatores explicativos do crescimento econômico e dos diferenciais de rendimento.

  6. La gestión de los recursos humanos en el marco de la calidad total

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    María Sol Castro

    1998-12-01

    Full Text Available Las empresas que han implantado la Gestión de la Calidad Total (CGT y que desean sostenerla con éxito, tienen en el tratamiento del lado humano de la calidad una oportunidad única para que los departamentos de recursos humanos (DRH desempeñen un papel protagonista. La necesidad de poner en práctica acciones específicas de gestión de los, recursos humanos sitúa a los DRH en una posición auténticamente estratégica. Les compete la creación y la puesta en marcha de un conjunto de procesos interdependientes de gestión que sirven de apoyo para el desarrollo de la GCT.

  7. La eficacia del sistema internacional de protección de derechos humanos de personas migrantes

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    Alex Munguía Salazar

    2017-06-01

    Full Text Available Los derechos humanos de las personas trabajadoras migrantes han sido llevados al ámbito vigente del derecho público, y se ha priorizando su carácter laboral. Este movimiento en pro de los derechos humanos de migrantes podemos evidenciarlo a partir de la entrada en vigor de la Convención Internacional sobre la Protección de los Derechos de Todos los Trabajadores Migrantes y sus Familias el 1 de julio de 2003, documento donde se plasman y fusionan dos cuerpos normativos internacionales, los derechos laborales de la OIT y los derechos humanos de la ONU, para fortalecer la vigencia de tales derechos en estos grupos trabajadores migrantes.

  8. A questão do humano e o animal rationale

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    Pivatto, Pergentino Stefano

    2006-01-01

    Full Text Available A especificação de lógico e político no animal rationale serviu historicamente como determinativo da dimensão humana, e o humano resumiu-se no desenvolvimento destas faculdades. Esta interpretação é contestada no século XX por Scheler, Arendt e Levinas, entre outros, que repõem a questão da relação entre ser, natureza e humano. A problematização ontológico-naturalista leva a repensar a crença naturalista sobre a qual repousa o pensamento antropológico ocidental e desafia a ressignificar o humano a partir de novas compreensões, sobretudo a partir da alteridade

  9. Corpo e pós-corpo: Cyborg e pós-humano

    OpenAIRE

    Andrade, Pedro José de Oliveira

    2015-01-01

    Corpos pós-humanos: Mashup do homem e da máquina; Visão melhorada; Prótese para a e-beleza; Renascimento em capela sistina digital? Corpos culturais; Cyborgs; Donna Haraway; Pós-humano; Projeto 1: Digitalização do conhecimento; Os novos heróis da cena social e os novos vilões; Crítica pelo humor: humanidade aumentada? Máscara digital; E-sexualidades. Humano-Animal-Máquina: Corpo e sobrevivência; Corpo e estética: Projeto 2: Novas estéticas do corpo; Projeto 3: Corpos e poder; Corpo e identida...

  10. Teoría Socialista de los Derechos Humanos Socialist Theory of Human Rights

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    Antonio Salamanca Serrano

    2011-05-01

    Full Text Available Este artículo presenta los principales postulados de la Teoría Socialista de los Derechos Humanos (TSDH, aplicación al ámbito de los Derechos Humanos del trabajo Teoría Socialista del Derecho, 2 vols. (Quito: Editorial Jurídica del Ecuador, 2011. Postulados científicos de un nuevo paradigma en Teoría de los Derechos Humanos que se somete a probación y verificación  histórica. La TSDH es un paradigma iusmaterialista que tiene sus diferencias con el iusnaturalismo, con el socio-contractualismo y con el iusvoluntarismo jurídicos. La TSDH arranca del hecho de hechos (de la última instancia que es la vida de los pueblos como praxis material histórica de realidad. En la TSDH el método es el materialismo histórico-dialéctico científico. El fundamento de los derechos humanos se encuentra en la obligación que impone la materia viva a los pueblos de satisfacer su sistema de necesidades/capacidades materiales para poder producir y reproducir sus vidas. Entiende a los derechos humanos como el satisfactor jurídico del sistema de necesidades/capacidades de los pueblos. Sus principales postulados caracterizan a la TSDH como una: (1º Teoría de la vida de los pueblos;  (2º Teoría de la praxis histórica (praxeológica; (3º Teoría materialista; (4º Teoría moral; (5º Teoría política; (6º Teoría jurídica; (7º Teoría revolucionaria. Palabras clave: Teoría. Derechos Humanos de los Pueblos. Iusmaterialismo. Socialismo.  Revolución.

  11. Aporias da fundamentação convencional dos direitos humanos e os caminhos da interculturalidade

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    Vicente de Paulo Barretto

    2017-04-01

    Full Text Available Resumo: A fundamentação dos direitos humanos é uma tarefa própria do campo de investigação filosófica que pretende, de um lado, refletir sobre a razão da existência desses direitos e, de outro, estabelecer os argumentos que lhe conferem validade e legitimidade.  Neste trabalho não se pretende responder diretamente às questões de existência e legitimidade dos direitos humanos. Os objetivos são, de um lado, demonstrar que a fundamentação é condição para a efetividade dos direitos humanos e, de outro, que existe um discurso convencional de fundamentação, filiado a pressupostos teóricos de matriz racional-individualista, que é, ao mesmo tempo, dominante e incongruente porque restringe as possibilidades de proteção universal desses direitos. Dominante porque a ele se filia grande parte dos jusfilósofos do Ocidente, entre eles o próprio Norberto Bobbio, os quais produzem a mais vasta e influente literatura sobre o tema. Incongruente porque o conceito de humanidade proposto pelo marco racional-individualista falha ao justificar a pretensa universalidade dos direitos humanos. A apropriação da noção de interculturalidade, por outro lado, pode constituir o ponto de partida para a construção de discursos de fundamentação que estejam atentos a realidades culturalmente plurais. O presente artigo é resultado parcial das investigações do Projeto de Pesquisa CNPq-Universal n. 476114/2013-6 Os Direitos Humanos no contexto Latino-americano: do caráter universal à interculturalidade integrado pelos autores. Palavras-chave: Fundamentação. Direitos humanos. Racionalidade. Individualismo. Interculturalidade.

  12. Cepas de Campylobacter jejuni resistentes a quinolonas aisladas de humanos, gallinas y pollos

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    Rodolfo Notario

    2011-08-01

    Full Text Available Se compararon 8 aislamientos de Campylobacter jejuni provenientes de humanos con enfermedad diarreica aguda, con 23 aislamientos de cloaca de gallinas y pollos obtenidos de zonas próximas a la ciudad de Rosario, todos resistentes a la ciprofloxacina. Las muestras se sembraron en agar selectivo y se incubaron en microaerofilia a 42 °C. Las colonias se identificaron con el método tradicional. Los aislamientos se conservaron a -70 °C en caldo cerebro corazón con 17% v/v de glicerina. La clonalidad se determinó por RAPD-PCR, utilizando el primer 1254 (Stern NJ. Se interpretaron los aislamientos como clones distintos cuando diferían en una banda de amplificación. Se obtuvieron 5 clones diferentes. Los patrones I, II y V fueron aislados en criaderos industriales de pollos y en humanos (el II también en un establecimiento de gallinas ponedoras de huevos. En un gallinero familiar se obtuvo el patrón I. El patrón III sólo se obtuvo de humanos. El patrón IV se halló en uno de los criaderos pero no en humanos. Se pudo determinar que 93.5% de las cepas se aislaron tanto de animales como de humanos, por lo que se considera posible que la colonización de criaderos con cepas resistentes a los antimicrobianos pudiera ser el origen de la infección de humanos.

  13. Anticuerpo anti-Dectin-1 humano, hibridoma productor de dicho anticuerpo y sus aplicaciones

    OpenAIRE

    Llorente Gómez, María de las Mercedes; Fernández Ruíz, Elena; Galán Díez, Marta; Kremer, Leonor; Casasnovas, José María

    2008-01-01

    Anticuerpo anti-Dectin-1 humano, hibridoma productor de dicho anticuerpo y sus aplicaciones. La presente invención consiste en el hibridoma MGD3 y el anticuerpo monocional producido por él (denominado también MGD3), que reconoce de forma especifica al receptor de membrana Decti-1 humano. El anticuerpo MGD3 es capaz de inhibir la unión de Dectin-1 a su ligando natural, los -glucanos que son componentes de la pared de los hongos. Además, bloquea específicamente la un...

  14. El comportamiento humano en las ciencias sociales: un enfoque económico institucional

    OpenAIRE

    Caballero Míguez, Gonzalo

    2005-01-01

    La Nueva Economía Institucional ha emergido en las últimas décadas del siglo XX como uno de los más sólidos programas de investigación en economía. Este programa modificó los supuestos de racionalidad sustantiva que sobre el comportamiento humano asumía la corriente principal en economía. Este artículo desarrolla los fundamentos teóricos que sobre el comportamiento humano sostiene el enfoque de la Nueva Economía Institucional. Las nociones de racionalidad limitada, modelos ment...

  15. Vacinação contra papilomavírus humano

    OpenAIRE

    Mello,Claudia Figueiredo

    2013-01-01

    A infecção pelo papilomavírus humano é comum e produz manifestações diversas. Essa infecção adquire importância para saúde pública, pois tem sido associada a malignidades do trato genital em homens e mulheres. Atualmente, temos disponíveis duas vacinas para prevenção da infecção pelo papilomavírus humano e, consequentemente, de doenças a ele associadas.

  16. Derecho humano al agua y al saneamiento: derechos estrechamente vinculados al derecho a la vida

    OpenAIRE

    Fernández Aller, Maria Celia; Luis Romero, Elena de; Guzmán Acha, Cristina

    2013-01-01

    Los derechos humanos al agua y al saneamiento están íntimamente relacionados con otros derechos esenciales para la vida, como la alimentación o la salud. Su reconocimiento como derechos humanos en 2010 marca un hito importante en el respeto, protección y realización de estos derechos, que se encuentran seriamente vulnerados para una mayoría de población más vulnerable y empobrecida, sobre todo en el ámbito rural. Mucho camino queda aún para conseguir no sólo la disponibilidad de agua y saneam...

  17. Perspectiva antropológico-filosófica del desarrollo humano

    OpenAIRE

    Guarín Jurado, Germán

    2018-01-01

    La aclaración de concepto de desarrollo humano, tal como oportuamente lo plantean los profesores Rafael Campo y Mariluz Restrepo en el texto La Educación en el Desarollo Humano, remite a dar una respuesta, o mejor, a precisar el sentido emanado de una primaria pregunta por el hombre; de ahí que necesariamente, bien lo dicen los maestros aludidos, haya que recurrir a la Antropología Filosófica. De este modo, cabe agregar a las ideas expuestas por Campo y Restrepo, algunas de las elucidaciones ...

  18. Mi experiencia en COMISEDH - Comisión de Derechos Humanos

    OpenAIRE

    Leal Lillo, María

    2017-01-01

    Treball final de Màster Universitari en Cooperació al Desenvolupament (Pla de 2015). Codi: SRP531. Curs acadèmic 2016-2017 En este trabajo se presenta la experiencia de prácticas externas del Máster en Cooperación al Desarrollo de la Universidad Jaume I, llevada a cabo en COMISEDH-Comisión de Derechos Humanos, una asociación civil sin ánimo de lucro con 38 años de experiencia en la lucha por los derechos humanos en Perú. A lo largo de los distintos apartados se presenta la entidad y el con...

  19. Objetividad jurídica, derechos humanos y estado de excepción

    OpenAIRE

    Cantú Martínez, Silvano

    2010-01-01

    La objetividad jurídica moderna fracasa ante la normalización creciente de la suspensión y/o restricción selectiva del derecho (particularmente de las reglas democráticas y los derechos humanos) en aras del derecho mismo. Para desenvolver este argumento, esta investigación explora: 1. La regularidad del antagonismo manifiesto a través del estado de excepción y los derechos humanos en la modernidad; 2. El análisis de la regulación jurídica internacional de los estados de excepción; y 3. La car...

  20. Panorama internacional de los derechos humanos de la mujer: una mirada desde Colombia

    OpenAIRE

    Carolina Angulo; José Manuel Luque

    2008-01-01

    Los estudios de género dan una perspectiva de las condiciones de vida de la mujer que en ocasiones dista de los derechos reconocidos en las normas tanto de carácter internacional como interno. La equidad de género y los derechos humanos de la mujer originan constantes debates que cuestionan la capacidad para ofrecer una protección efectiva a las mujeres. Los conceptos tradicionales de derechos humanos se han desarrollado desde una representación androcéntrica, que en much...

  1. El bienestar laboral y el talento humano en la Institución educativa Eduardo Santos

    OpenAIRE

    Restrepo Parra, Martha María, Hna.; Rodríguez Torres, Rafael Alberto; Rojas López, Yaneth, Hna.

    2013-01-01

    Tesis (Especialista en Gerencia Educativa). Universidad Católica de Manizales, Facultad de Educación, 2013 Con grandes expectativas se presenta el Proyecto Bienestar Laboral para el Talento Humano a la Institución Educativa Eduardo Santos. El cual nace de la necesidad de aplicar los conocimientos teóricos – prácticos de la especialización en Gerencia Educativa y la necesidad de tener un plan de acción que fortalezca en esta Institución el talento humano que lleve a mejorar las relaciones s...

  2. Direitos humanos na educação: superar os desafios

    OpenAIRE

    Karen Franklin

    2010-01-01

    Resumo:Este artigo apresenta reflexões sobre as dificuldades e os desafios da educação diante da questão dos direitos humanos. São apresentados alguns aspectos fundamentais para reflexão: o princípio de universalidade dos direitos humanos e o papel da filosofia no esclarecimento da experiência ética e da cidadania. Apresentamos algumas concepções necessárias para a conquista do conceito de humanidade atrav&eacu...

  3. Arnold Gehlen, reflexões sobre ser humano

    Arnold Gehlen, reflections on human being

    OpenAIRE

    Javier Ignacio Vernal

    2009-01-01

    PortuguesEste manuscrito trata da concepção de Arnold Gehlen sobre ser humano. A tese de Gehlen é a do homem biologicamente deficiente que através da atividade de transformação da matéria e do meio de acordo com suas necessidades, se constitui diverso dos outros animais. Esse agir humano, que gera a distância progressiva em relação aos outros animais, pressupõe a abertura especificamente humana para o mundo. A cultura é considerada por Gehlen como o espaço e o ambiente necessários para a vida...

  4. Tres experiencias para pensar la educación en derechos humanos en Colombia

    OpenAIRE

    Rodriguez Heredia, Douglas Giovany

    2015-01-01

    A lo largo de esta investigación se analizaron comparativamente tres experiencias auto-definidas como de Educación en Derechos Humanos, a saber, una organización no gubernamental, la Escuela de Derechos Humanos de Cinep; un colegio oficial de la ciudad de Bogotá, la IED Eduardo Umaña Mendoza; y un movimiento social, el Movimiento Nacional de Víctimas de Crímenes de Estado-Capítulo Bogotá. En lo fundamental, se analizaron los procesos de constitución de cada escenario, los discursos, y las prá...

  5. Hume e o entendimento humano:estudo introdutório

    Directory of Open Access Journals (Sweden)

    Adriano Naves de Brito

    2007-12-01

    Full Text Available Este trabalho é um estudo introdutório e didático da concepção humeana do entendimento humano.Na primeira parte apresento a concepção empirista em que Hume se inscreve, na segunda são sumariados os principais aspectos de sua teoria acerca do entendimento humano e na última parte teço algumas considerações a propósito do papel da linguagem e do juízo nesta teoria. 

  6. La eficacia del sistema internacional de protección de derechos humanos de personas migrantes

    OpenAIRE

    Alex Munguía Salazar

    2017-01-01

    Los derechos humanos de las personas trabajadoras migrantes han sido llevados al ámbito vigente del derecho público, y se ha priorizando su carácter laboral. Este movimiento en pro de los derechos humanos de migrantes podemos evidenciarlo a partir de la entrada en vigor de la Convención Internacional sobre la Protección de los Derechos de Todos los Trabajadores Migrantes y sus Familias el 1 de julio de 2003, documento donde se plasman y fusionan dos cuerpos normativos internacionales, los der...

  7. Los derechos humanos y su análisis desde una perspectiva crítica

    OpenAIRE

    Mora Calvo, María Fernanda

    2014-01-01

    El presente documento constituye el trabajo final de investigación de la Maestría en Derechos Humanos de la Universidad Estatal a Distancia, referido a la temática de los Derechos Humanos y su análisis desde una perspectiva crítica. El mismo contiene el planteamiento del tema, problema a investigar, objeto y objetivos. Además de un breve acercamiento a los antecedentes, en forma de estado de la cuestión, recuperando los aportes de investigaciones y trabajos finales de graduación en relació...

  8. EFEITO DE CÁLCULOS BILIARES HUMANOS NA CAVIDADE PERITONEAL DE RATOS

    OpenAIRE

    Orlando Jorge Martins Torres; José Aparecido Valadão; Ailton José Rodrigues Silva; Rosane Penha Macau; Jean Carlos Antunes Cintra; Ulrich Andreas Dietz; Paulo Afonso Nunes Nassif

    1998-01-01

    Este estudo tem por objetivo analisar experimentalmente as características macroscópicas e microscópicas de cálculos biliares humanos na cavidade peritoneal de ratos. Foram utilizados 32 ratos Wistar, machos, pesando entre 205 e 268 g. Estes animais foram distribuídos em dois grupos e o procedimento cirúrgico foi realizado em cada grupo: no grupo A (n =16), os animais foram submetidos a manipulação intestinal; no grupo B (n=16), cálculos biliares humanos foram colocados na cavidade peritoneal...

  9. El estado del arte en las infecciones producidas por el virus del papiloma humano

    OpenAIRE

    Julio César Reina; Nubia Muñoz; Gloria Inés Sánchez

    2008-01-01

    La infección anogenital por el virus del papiloma humano (VPH) es la infección de transmisión sexual más frecuente en todo el mundo. En los humanos se han identificado alrededor de 100 genotipos de los cuales unos 40 infectan la región ano-genital. Los 15 genotipos de alto riesgo, son causa necesaria del cáncer cervical y han sido implicados como agentes carcinogénicos de la vulva, vagina, pene, ano y región orofaríngea. Los VPH de bajo riesgo producen las verrugas genitales (condilomas) y la...

  10. Sistema social humano-cão a partir da autopoiese em Maturana

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    Faraco, Ceres Berger

    2010-01-01

    Full Text Available Neste artigo propomos uma ampliação da noção de social na teoria de Maturana objetivando compreender o sistema social humano-cão. Apresentamos alguns conceitos sobre os sistemas sociais e os articulamos às propriedades desta relação que constituem um novo domínio de realidade: a legitimação do outro na relação e o acoplamento a partir de história de coordenações consensuais recorrentes entre o humano e o cão, produzindo um novo domínio social

  11. Entre la educación en derechos humanos y los derechos humanos escolares: una aproximación desde la perspectiva de los saberes escolares

    Directory of Open Access Journals (Sweden)

    Leidy Viviana Muñoz Hurtado

    2016-03-01

    Full Text Available Comprender la insuficiencia de la educación en derechos humanos como herramienta analítica implica definir el campo simbólico que rodea, desde hace más de tres décadas, su institucionalización. Sin lugar a dudas, la principal razón para impugnar la idea de educación en derechos humanos como un saber escolar —sin que por ello no permita reflexionar acerca de diversas relaciones entre escuela y derechos humanos— tiene que ver con que no se produce en el marco de la escuela misma, e incluso, a diferencia de otras disciplinas o formas de conocimiento, las apropiaciones por parte de los sujetos educativos han dado lugar a nuevas tensiones que generan dislocaciones en la escuela misma.

  12. Programa de gestion de talento humano en una empresa de conocimiento intensivo (kif), a partir de la estrategia corporativa

    OpenAIRE

    Espol; Coello Ortiz, Catalina Del Carmen; Villagomez Uraga, Daniela

    2015-01-01

    Este documento trata de un programa de gestión de talento humano realizado a una empresa, realizado a partir del diagnóstico organizacional, se redefinió la estrategia corporativa, con su cadena de valor y estrategia de negocios. Guayaquil Magister en Gestión De Talento Humano

  13. Pueblos indígenas, autonomía jurisdiccional y Derechos Humanos. Vídeo 1/2

    OpenAIRE

    Borja Jiménez, Emiliano; Seminario 60 aniversario DUDH, Instituto Universitario de Derechos Humanos Universitat de València

    2008-01-01

    Conferencia ''Pueblos indígenas, autonomía jurisdiccional y Derechos Humanos'' Seminario 60 aniversario DUDH, Instituto Universitario de Derechos Humanos Universitat de València (Facultat de Dret UV, 18 noviembre 2008).Duración: 30M

  14. Pueblos indígenas, autonomía jurisdiccional y Derechos Humanos. Vídeo 2/2

    OpenAIRE

    Borja Jiménez, Emiliano; Seminario 60 aniversario DUDH, Instituto Universitario de Derechos Humanos Universitat de València

    2008-01-01

    Conferencia ''Pueblos indígenas, autonomía jurisdiccional y Derechos Humanos'' Seminario 60 aniversario DUDH, Instituto Universitario de Derechos Humanos Universitat de València (Facultat de Dret UV, 18 noviembre 2008).Duración: 8M

  15. Induction of local and systemic immune reactions following infection of turkeys with avian Metapneumovirus (aMPV) subtypes A and B.

    Science.gov (United States)

    Liman, Martin; Rautenschlein, Silke

    2007-02-15

    Most of the studies regarding the immunopathogenesis of avian Metapneumovirus (aMPV) have been done with subtype C of aMPV. Not much is known about the immunopathogenesis of aMPV subtypes A and B in turkeys. Specifically, local immune reactions have not been investigated yet. We conducted two experiments in commercial turkeys. We investigated local and systemic humoral and cell mediated immune reactions following infection with an attenuated vaccine strain of aMPV subtype B (Experiment I) and virulent strains of aMPV subtypes A and B (Experiment II). Turkeys infected with virulent aMPV strains developed mild respiratory signs while birds inoculated with the attenuated aMPV did not show any clinical signs. Virus neutralizing antibodies were detected locally in tracheal washes and systemically in serum as soon as 5-7 days post aMPV infection (PI) independent of the strain used. Virus neutralizing antibody titres peaked at 7 days PI and then antibody levels declined. The peak of serum ELISA antibody production varied between infected groups and ranged from 14 and 28 days PI. All aMPV strains induced an increase in the percentage of CD4+ T cell populations in spleen and Harderian gland at days 7 or 14 PI. Furthermore, as shown in Experiment I, infection with the attenuated aMPV-B strain stimulated spleen leukocytes to release significantly higher levels of interferons (IFNs), interleukin-6 and nitric oxide in ex vivo culture in comparison to virus-free controls up to 7 days PI (P<0.05). As detected by quantitative real time RT-PCR in Experiment II, infection with virulent aMPV induced an increased IFNgamma expression in the Harderian gland in comparison to virus-free controls. IFNgamma expression in the spleen varied between aMPV strains and days PI. Overall, our study demonstrates that aMPV subtypes A and B infection induced humoral and cell mediated immune reactions comparable to subtype C infections. We observed only temporary stimulation of serum virus neutralizing

  16. Vaccination of commercial broiler chicks against avian metapneumovirus infection: a comparison of drinking-water, spray and oculo-oral delivery methods.

    Science.gov (United States)

    Ganapathy, Kannan; Bufton, Andrew; Pearson, Andrew; Lemiere, Stephane; Jones, Richard C

    2010-05-21

    Avian metapneumovirus (aMPV) has become an important cause of viral respiratory infections in turkey and chickens. Live and inactivated vaccinations are available worldwide for prevention of disease and economic losses caused by this pathogen. The efficacy of these vaccines is vigorously tested under laboratory conditions prior to use in the field. In this study, a live subtype B aMPV vaccine was administered by spray, drinking water or oculo-oral methods to separate groups of broiler chicks under field conditions. Following this, the chicks were immediately transferred to separate rooms in an experimental isolation house, monitored and challenged with virulent subtype B aMPV. No clinical signs were recorded following the vaccination methods. In the oculo-oral vaccinated chicks, 40-60% of the birds were vaccine virus positive by RT-PCR. In addition, in comparison to other groups, statistically higher levels of aMPV ELISA antibodies were detected. After spray vaccination, the number of chicks positive for the vaccine virus increased gradually from 10% at one week to 30% by 3 weeks post vaccination. Following drinking water vaccination, 30% of chicks were aMPV positive at 1 week but negative by 3 weeks post vaccination. In both, spray and drinking water vaccinated groups, no ELISA antibodies were detected, but when challenged all chicks were protected against disease. At 5 days post challenge, 100% of chicks in the unvaccinated and those vaccinated by spray or drinking water routes but only 20% of the oculo-oral-vaccinated chicks were aMPV positive by RT-PCR. At 10 days post challenge, 10% of chicks in each group were aMPV RT-PCR positive. On challenge, all vaccinated chicks were protected against disease. It appears that when aMPV vaccine is accurately applied to chicks by spray or drinking water routes, both are capable of giving protection against clinical disease equal to that induced in those chicks vaccinated individually by the oculo-oral route. Copyright 2010

  17. Perfeccionamiento de gestión de capital humano en Iberostar grand hotel Trinidad, Cuba

    Directory of Open Access Journals (Sweden)

    Analién Pelegrín Naranjo

    2017-12-01

    Full Text Available La investigación aborda una problemática relevante para el sistema empresarial y el Ministerio del Turismo en Cuba. El perfeccionamiento en la Gestión del Capital Humano de organizaciones que identifican “al hombre” como principal activo, portador de evidentes beneficios; las convierte en exitosas. El objetivo del estudio consiste en plantear una tecnología para el diseño del Sistema de Gestión de Capital Humano con enfoque a procesos en Iberostar Grand Hotel Trinidad, de donde se induce la novedad científica; esta tecnología incluye un modelo, procedimientos general y específicos respectivos a cada uno de los nueve procesos clave de la Gestión del Capital Humano. La posterior implementación del Sistema diseñado garantizará la efectiva gestión de los procesos, retroalimentación y mejora continua, dotando a los trabajadores de un desempeño laboral superior, en la prestación de un servicio de mayor calidad en aras de lograr plena satisfacción de los clientes del hotel. Palabras clave: tecnología, diseño, gestión de capital humano, enfoque a procesos.

  18. Los retos del desarrollo humano en el territorio: experiencias en Brasil y Colombia

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    Sueli de Lourdes Couto Rosa

    2012-01-01

    Full Text Available En este artículo se reflexiona sobre el papel del Estado y el territoriocuando se están abordando procesos de desarrollo humano local. Para esto se analizó el desarrollo, los sustentos teóricos del desarrollo humano local (DHL y la metodología propuesta por el Programa de Naciones Unidas para el Desarrollo para su consecución en lo local. Finalmente, se hace una revisión de los resultados de la aplicación de la metodología de DHL en Colombia y Brasil, lo que permite concluir que el DHL se diferencia de las demás formas de desarrollo por la formación de capital social y humano, por la generación de activos de ciudadanía para las personas involucradas en el proceso. Sin embargo, los grandes desafíos siguen. Sin el imperativo real por el desarrollo humano ysin el compromiso con un desarrollo desde su base territorial,es probable que la mayoría de los proyectos de DHL sean llevados por el mismo camino de los demás modelos de desarrollo.

  19. Retos y rol estratégico en la gestión del talento humano

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    Juan Carlos Aguilar Joyas

    2016-03-01

    Full Text Available La gestión estratégica del talento humano, como se conoce en las organizaciones de hoy, se caracteriza por la coherencia o el ajuste entre su papel y la estrategia organizacional. Partiendo de esta premisa se desarrolló la investigación para conocer el papel, los retos y las prácticas en la gestión del talento humano (GTH de 16 empresas de la ciudad de Cali, logrando poner en evidencia que estas empresas han tenido avances en cuanto a las prácticas utilizadas para el desarrollo del talento humano frente a la competitividad y las tendencias del mercado, pero igualmente se enfrentan a grandes desafíos en el sentido de fortalecer su orientación estratégica y la medición del valor; se destaca también que los programas de bienestar, la medición de la efectividad del talento humano, los procesos de subcontratación y la compensación son aspectos sensibles en muchas de ellas

  20. Estimación del Índice de Desarrollo Humano ajustado para los departamentos colombianos

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    Jorge Raúl Ramírez Zambrano

    2015-07-01

    Full Text Available La presente investigación tiene como objetivo estimar dos índices de desarrollo humano ajustados para Colombia. La estimación se realiza para el periodo 2002-2010 y cubre trece departamentos colombianos, a fin de revelar el efecto que produce tanto la informalidad laboral como la educación media y terciaria en el desarrollo humano. El enfoque y la metodología utilizados son los presentados por el Programa de las Naciones Unidas para el Desarrollo (PNUD, pero incluye una variante en la construcción de los índices de logro educativo y el PIB per cápita. Los resultados evidencian que la informalidad laboral deteriora el desarrollo humano, mientras que una mayor cobertura en educación media y en educación superior ejerce una influencia positiva en los logros medidos a través del índice de desarrollo humano ajustado para Colombia. Lo anterior es fundamental a la hora de establecer recomendaciones de política bajo una perspectiva regional y territorial.

  1. Instrumentos jurídicos para mejorar la protección de los derechos humanos

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    Marcial Rubio Correa

    1984-12-01

    Full Text Available Existe un paso distinto en el desarrollo de los derechos humanos en el ámbito del Derecho Internacional, y en el ámbito del Derecho interno delos Estados, con una clara ventaja del primerode ambos. Esto significa, sin embargo, que los derechos humanos constituyen declaraciones que, en muchos casos. no pueden ser hechas realidad por la falta de mecanismos jurídicos internos que los hagan efectivos.El autor propone diversas alternativas a ser consideradas tanto en el plano constitucional, como en los planos legislativo e institucional de los Estados, en la perspectiva de encontrar formas de garantía jurídica de los derechos humanos, más allá del Habeas Corpus y la Acción de amparo. Las propuestas tratan, fundamentalmente, con mecanismos hermenéuticos del Derecho, fortalecimiento del sistema democrático, y creación y desarrollo de instituciones con competencia privativa en materia de protección y vigilancia del cumplimiento de los derechos humanos.

  2. Estimación del Índice de Desarrollo Humano ajustado para los departamentos colombianos

    Directory of Open Access Journals (Sweden)

    Jorge Ramírez

    2015-01-01

    Full Text Available Resumen: La presente investigación tiene como objetivo estimar dos índices de desarrollo humano ajustados para Colombia. La estimación se realiza para el periodo 2002-2010 y cubre trece departamentos colombianos, a fin de revelar el efecto que produce tanto la informalidad laboral como la educación media y terciaria en el desarrollo humano. El enfoque y la metodología utilizados son los presentados por el Programa de las Naciones Unidas para el Desarrollo (PNUD, pero incluye una variante en la construcción de los índices de logro educativo y el PIB per cápita. Los resultados evidencian que la informalidad laboral deteriora el desarrollo humano, mientras que una mayor cobertura en educación media y en educación superior ejerce una influencia positiva en los logros medidos a través del índice de desarrollo humano ajustado para Colombia. Lo anterior es fundamental a la hora de establecer recomendaciones de política bajo una perspectiva regional y territorial.

  3. Panorama internacional de los derechos humanos de la mujer: una mirada desde Colombia

    Directory of Open Access Journals (Sweden)

    Carolina Angulo

    2008-01-01

    Full Text Available Los estudios de género dan una perspectiva de las condiciones de vida de la mujer que en ocasiones dista de los derechos reconocidos en las normas tanto de carácter internacional como interno. La equidad de género y los derechos humanos de la mujer originan constantes debates que cuestionan la capacidad para ofrecer una protección efectiva a las mujeres. Los conceptos tradicionales de derechos humanos se han desarrollado desde una representación androcéntrica, que en muchos casos ignora las diferencias entre hombres y mujeres. Por consiguiente, la necesidad de la revisión del tema de los Derechos Humanos desde una perspectiva de género impone obligaciones a los estados como garantes del bienestar de los individuos. Analizar los Derechos Humanos de la Mujer en Colombia exige entender por qué, a pesar de los esfuerzos realizados y los logros alcanzados, aún persiste la incapacidad para ofrecer una protección efectiva a las mujeres, sobre todo a las indígenas, afrodescendientes y campesinas, tanto en tiempos de paz como durante el conflicto armado.

  4. Crepes & Waffles: Una filosofía del talento humano puesta en marcha

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    Nancy Calixto Sandoval

    2006-07-01

    Full Text Available Describe la receta gerencial que ha llevado al éxito a Crepes & Wafles: una muy especial concepción de la gerencia de recursos humanos basada en cuatro principios fundamentales: paz, amor, armonía y felicidad

  5. Consideraciones sobre el factor humano en dirección de la empresa colombiana

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    Enrique Rugeles Amaya

    1988-04-01

    Full Text Available RESUMEN El factor humano es importante para el éxito empresarial, ya que en general las universidades hacen énfasis en la planeación, organización, coordinación, dirección y control.

  6. Manipulación genética de seres humanos

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    Manuel Santos Alcántara

    2006-08-01

    Full Text Available El gran avance que ha tenido la Genética en los últimos años y, particularmente, aquello relacionado con el desciframiento del genoma humano, ha traído a la discusión pública la posibilidad concreta de manipular genéticamente a los seres humanos. El mejoramiento o perfeccionamiento genético de los seres humanos, denominado eugenesia, actualmente se ha convertido técnicamente en una realidad, motivando una profunda reflexión de tipo ético. La pregunta básica es la siguiente: aquello que es técnicamente posible de realizar ¿es ético hacerlo? ¿Tienen derecho los padres a acceder a la tecnología genética para mejorar las características de sus hijos? En este artículo se revisan las bases científicas del mejoramiento genético de los seres humanos, y se plantean los cuestionamientos éticos más relevantes derivados de esta manipulación.

  7. Derechos humanos y emociones desde una perspectiva de los colonizados: Antropofagia, Surrealismo Legal y Estudios Subalternos

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    José Manuel Barreto

    2014-12-01

    Full Text Available El Tercer Mundo puede experimentar fácilmente una existencia fantasmal: hablamos pero no somos oídos. No obstante, en nuestra cultura hay numerosas tendencias y posiciones que son relevantes para la tarea de pensar a los derechos humanos desde una perspectiva distinta. Entre ellos, las críticas al racionalismo desarrolladas por Oswald de Andrade y Luis Alberto Warat en Brasil y Argentina –en las que existe la posibilidad de integrar las emociones en la teoría de los derechos humanos. Compartiendo la preocupación por aquellos excluidos del “orden mundial” y el llamado a la sensibilidad, los Estudios Subalternos desarrollaron ideas que apunta a establecer una relación entre colonialismo, derechos humanos y sufrimiento. Este es el caso del trabajo de Upendra Baxi, quien ha realizado una crítica a lasteorías occidentales del derecho y ha elaborado un encuentro fructífero entre los aportes de los Estudios Subalternos y la teoría de los derechos humanos.

  8. Efecto citopático por el Virus del Papiloma Humano (HPV)

    OpenAIRE

    Spinelli, Osvaldo Mateo; Altamirano, Eugenia M.

    2003-01-01

    El Papiloma Virus Humano (HPV) es un virus DNA de 55 nanómetros de diámetro, sin envoltura y con una estructura icosahédrica constituida por 72 capsómeros. En su interior se encuentra el genoma viral compuesto por una doble cadena circular de DNA de alrededor de 7900 pares de bases nucleotídicas.

  9. Ações afirmativas da perspectiva dos direitos humanos Affirmative action from a human rights perspective

    Directory of Open Access Journals (Sweden)

    Flavia Piovesan

    2005-04-01

    Full Text Available Objetiva o artigo desenvolver uma análise a respeito das ações afirmativas sob a perspectiva dos direitos humanos. Inicialmente, trata da concepção contemporânea de direitos humanos, introduzida pela Declaração Universal de 1948, com ênfase na universalidade, indivisibilidade e interdependência dos direitos humanos. Em um segundo momento são apreciadas as ações afirmativas da perspectiva dos direitos humanos, com destaque dos valores da igualdade e diversidade. Por fim, são avaliadas as perspectivas e desafios para a implementação da igualdade étnico-racial na ordem contemporânea.The article aims to develop an analysis on affirmative action from a human rights perspective. Initially, it deals with the contemporary conception of human rights, introduced by the Universal Declaration of 1948, stressing their universality, indivisibility, and interdependence. At a second stage, affirmative action is analyzed from a human rights perspective, stressing the values of egalitarianism and diversity. Finally, the perspectives and challenges to implement ethnic-racial egalitarianism in the contemporary order are assessed.

  10. Qual seria a fonte de fungos miceliais encontrados em leite humano ordenhado?

    Directory of Open Access Journals (Sweden)

    Franz Reis Novak

    2002-06-01

    Full Text Available Caracterizou-se os gêneros de fungos miceliais encontrados em amostras de leite humano ordenhado, recebidas a partir de coleta domiciliar, pelo Banco de Leite Humano do Instituto Fernandes Figueira. Foram analisadas 821 amostras de leite humano ordenhado, obtidas ao acaso, a partir de frascos coletados nos domicílios pelas próprias doadoras, e realizadas pesquisas de bolores e leveduras e microrganismos mesófilos. As análises revelaram a ocorrência de bolores e leveduras em 43 (5,2% das amostras, com contagens atingindo a ordem de 10³UFC/ml, tendo sido isoladas 48 cepas de fungos miceliais, que foram identificadas por técnicas padrão de laboratório, como as seguintes: Aspergillus Grupo Niger (6,3%, Aspergillus sp. (4,2%, Paecilomyces sp. (12,6%, Penicillium sp. (60,4%, Rhizopus sp. (2,0% e Syncephalastrum sp. (14,5%. Discute-se a importância do controle da assepsia das mãos das doadoras, antes da coleta do leite humano.

  11. Qual seria a fonte de fungos miceliais encontrados em leite humano ordenhado?

    Directory of Open Access Journals (Sweden)

    Novak Franz Reis

    2002-01-01

    Full Text Available Caracterizou-se os gêneros de fungos miceliais encontrados em amostras de leite humano ordenhado, recebidas a partir de coleta domiciliar, pelo Banco de Leite Humano do Instituto Fernandes Figueira. Foram analisadas 821 amostras de leite humano ordenhado, obtidas ao acaso, a partir de frascos coletados nos domicílios pelas próprias doadoras, e realizadas pesquisas de bolores e leveduras e microrganismos mesófilos. As análises revelaram a ocorrência de bolores e leveduras em 43 (5,2% das amostras, com contagens atingindo a ordem de 10³UFC/ml, tendo sido isoladas 48 cepas de fungos miceliais, que foram identificadas por técnicas padrão de laboratório, como as seguintes: Aspergillus Grupo Niger (6,3%, Aspergillus sp. (4,2%, Paecilomyces sp. (12,6%, Penicillium sp. (60,4%, Rhizopus sp. (2,0% e Syncephalastrum sp. (14,5%. Discute-se a importância do controle da assepsia das mãos das doadoras, antes da coleta do leite humano.

  12. Los perfiles de exigencias en la ocupación del profesional de recursos humanos

    Directory of Open Access Journals (Sweden)

    Santiago Pereda Marín

    2003-01-01

    Full Text Available En este trabajo se presentan los resultados de un estudio exploratorio que se llevó a cabo en la Universidad Complutense de Madrid para definir el perfil de exigencias ocupacionales del profesional de recursos humanos. En este estudio se considera como profesional de recursos humanos al licenciado, ingeniero o doctor que desarrolla su actividad en el área de recursos humanos sin ocupar puestos directivos dentro de la estructura de la organización. Los resultados de este estudio, que se obtuvieron a partir de una muestra de 37 profesionales de recursos humanos en activo, señalan seis competencias como claves para el desempeño de la ocupación; éstas son, por orden de importancia: trabajo en equipo, orientación al cliente, planificación/organización, comunicación, flexibilidad y colaboración. Asimismo, se comprobó que la metodología empleada es útil, aunque se considera que es preciso introducirle pequeños cambios para optimizar el trabajo en la definición de los perfiles de exigencias ocupacionales.

  13. O Estado e os direitos humanos: uma visão em perspectiva

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    Medeiros, Maria Bernadette de Moraes

    2003-01-01

    Full Text Available Estudo sobre os sistemas de idéias que fundamentaram as ações dos sujeitos, nos diferentes contextos históricos, na conquista dos Direitos Humanos e Liberdades Fundamentais, no âmbito do Estado Moderno

  14. A emergência da responsabilidade criminal individual no Sistema Interamericano de Direitos Humanos

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    Raquel da Cruz Lima

    2012-01-01

    Full Text Available Este artigo analisa a emergência e difusão da regra da responsabilidade criminal individual por violações de direitos humanos a partir da jurisprudência contenciosa da Corte Interamericana de Direitos Humanos. Tendo como referência as pesquisas de Kathryn Sikkink sobre justiça de transição, procura-se identificar a maneira como o Sistema Interamericano de Direitos Humanos vem expandindo o sentido da responsabilidade criminal individual e também sugerir a incompatibilidade com a proteção dos direitos humanos que esse novo modelo pode ter.This paper analyzes the emergence and diffusion of the norm of individual criminal accountability for human rights violations from the perspective of the contentious jurisprudence of the Inter-American Human Rights Court. Taking as reference the research of Kathryn Sikkink on transitional justice, this paper seeks to identify how the Inter-American Human Rights System has been expanding the meaning of individual criminal accountability and also suggests the incompatibility with the protection of human rights that this new model may have.

  15. Derechos humanos constituyentes, luchas sociales y cotidianas e historización

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    David Sánchez Rubio

    2014-10-01

    Full Text Available Los derechos humanos tienen en su reclamación a uno de sus componentes más importantes. Se puede exigir un derecho negado pero ya reconocido por el ordenamiento jurídico de un estado constitucional de derecho o reivindicar un derecho nuevo que no está recogido por el sistema normativo de los estados-nacionales que incorporan internamente a los sistemas internacionales. Las causas de que se reclamen derechos residen en algún tipo de agravio, afrenta o perjuicio ocasionado a un ser humano particular o a un grupo o colectivo de seres humanos. Ese daño puede ser individual y puntual, producido por un acto concreto y determinado, o puede ser provocado por una relación desigual de poder más estabilizada, es decir, por una estructura o un sistema de dominación que genera la discriminación, la opresión, la exclusión y/o la muerte de quienes lo sufren. Por esta razón, para Ignacio Ellacuría la mejor forma y el método más adecuado para percibir un derecho humano real y dinámico es el de negar aquella condición de esclavitud, debilidad y opresión que viola una dimensión vital y existencia de los seres humanos que se traduce y significa en términos de dignidad, de libertad o de derechos, ya que este estado o condición negadora es la que ofrece un dato temático primario que sirve de fuente no solo de análisis, sino, principalmente, para poder ha-cer justicia enfrentándola y, de modo dialéctico, anulándola, mediante su superación crítica y transformadora.

  16. CAPITALES HUMANO Y NATURAL DISPONIBLE EN LA MICROCUENCA DEL RÍO MEMBRILLO

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    Wendy Ximena Andrade Cedeño

    2012-06-01

    Full Text Available El trabajo se desarrolló con el objetivo de caracterizar el capital humano y natural en la microcuenca del río Membrillo y analizar la relación que existe con las estrategias de vida utilizadas. La metodología para el análisis de las variables e indicadores se basó en el enfoque de capitales de las comunidades, donde se asigna el mismo grado de importancia a cada uno de los indicadores para la generación de bienestar. Los resultados muestran en la tipificación de las familias tres grupos de hogares, que se diferencian en el uso del capital humano y natural, lo cual se relacionan con sus estrategias de vida. El grupo dos posee mayor disponibilidad de los capitales, se evidencia que el humano, es el factor que determina la cantidad y calidad de la mano de obra disponible. En el grupo uno, los dos capitales se encuentran menos fortalecidos, el capital humano está interactuando negativamente con el natural, cuestión que se relaciona con las estrategias de vida, formación, capacitación e intervención hacia los recursos naturales. El grupo tres, se encuentra en una situación intermedia; sus integrantes presentan una mayor variabilidad en el aprendizaje, se destaca la siembra de árboles para conservar el agua y dar sombra a los animales que explotan productivamente. Como resultado de este trabajo se prevé la urgencia de trabajar con el capital humano para fortalecer el capital natural y lograr un equilibro sostenible en el tiempo.

  17. A guerra do sucesso pelos talentos humanos The battle of success to attract human talents

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    Jefferson Marco Antonio Leonardo

    2002-01-01

    Full Text Available A mudança no mundo empresarial atinge um elevado grau de inovação e agilidade, contemplando o ser humano como protagonista de uma nova história organizacional. Buscou-se constatar, na história e nas práticas atuais de administração, os referenciais de importância na gestão de recursos humanos em relação ao atendimento das necessidades e desejos dos colaboradores, como fonte estratégica de atração e retenção dos talentos humanos. A proposta para o sucesso sustentado das organizações está intimamente ligada a um redesenho na gestão de recursos humanos, transformando o atual tratamento em massa para uma gestão individualizada e diferenciada aos colaboradores, com o propósito de atender às novas exigências na retenção dos talentos humanos e da continuidade das empresas de resultados.The change in the business world reaches a high degree of innovation and agility, contemplating human being as the protagonist of a new organizational history. We've tried to demonstrate, based on history and current management practices, the most important references, in human resources management, related to the importance of attending the necessities and desires of collaborators, as a strategic source of attraction and retain of human talents. The sustained success of organizations is closed linked to a redesign in the human resources management techniques, transforming the current mass treatment to a individualized and differentiated administration related to the collaborators, with the intent of attending to new demands on human talents retaining and continuity of results oriented companies.

  18. El complejo mayor de histocompatibilidad humano: sistema HLA

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    Luis Fernando García

    1989-02-01

    Full Text Available

    El complejo mayor de histocompatibilidad humano, o sistema HLA, está localizado en el brazo corto del cromosoma 6. Sus genes codifican tres tipos de moléculas. Los antígenos clase I (HLA-A, B, C y E están formados por una cadena pesada unida no covalentemente a la β2-microglobulina y se expresan en la superficie de la mayoría de las células nucleadas del organismo. Estos antígenos actúan como elementos de restricción en la activación de los linfocitos T CD8+. Los antígenos clase II son dímeros compuestos por cadenas α y β y su distribución tisular está limitada sólo a algunos tipos de células. Estas moléculas actúan restringiendo la presentación de antígenos a los linfocitos CD4+. Los antígenos de clase III son proteínas plasmáticas del sistema del complemento. Los diferentes loci del sistema HLA son muy polimórficos y sus productos se heredan en bloques conocidos como haplotipos. Debido a que los diferentes grupos étnicos presentan variaciones en la frecuencia de ale ios y haplotipos, el HLA ha sido muy útil en los estudios antropogenéticos. Algunos antígenos HLA están presentes en pacientes con determinadas enfermedades con una frecuencia significativamente diferente a la encontrada en la población general. Estos hallazgos han sido de gran importancia para comprender la patogénesis y los mecanismos genéticos de resistencia o susceptibilidad a dichas enfermedades. En el campo de los transplantes de órganos, la compatibilidad HLA donante-receptor correlaciona con la sobrevida del injerto. El sistema HLA también parece tener mucha importancia en los fenómenos inmunológicos que ocurren durante el

  19. Ensino de biologia e educação ambiental : uma leitura peirceana das formas de relação dos animais humanos com os não humanos

    OpenAIRE

    Adriana Ribeiro Ferreira Rodrigues

    2015-01-01

    Esta pesquisa discute as formas de relação dos seres humanos com os animais não humanos a partir da perspectiva da semiótica peirceana, do ensino de Biologia, da educação científica e da Educação Ambiental. O problema se constituiu em questionar qual a gama de entendimentos dos alunos e alunas do Ensino Médio acerca das relações dos seres humanos com os animais como signos produtores de interpretantes no contexto de um ensino dos conteúdos sobre vertebrados com abordagem ambiental. E, ainda, ...

  20. La gestión del talento humano y su aporte estratégico en los objetivos organizacionales del Servicio de Rentas Internas, con sustento en el Cuadro de Mando del Talento Humano

    OpenAIRE

    Toapanta Guambi, Álvaro Hernán

    2013-01-01

    147 hojas : ilustraciones, 29 x 21 cm + CD-ROM 5055 La presente tesis tiene el propósito de evidenciar el aporte estratégico de la gestión del Talento Humano en la consecución de los objetivos organizacionales sustentado en el cuadro de mando de talento humano para una institución pública, basado en el conocimiento de los 7 pasos para medir la influencia del talento humano de (Becker, Huselid, and Ulrich, 2001) y cuadro de mando para instituciones públicas. El esquema desarrollado en es...

  1. A Convenção 158 da OIT à luz da teoria constitucional dos tratados internacionais sobre direitos humanos

    OpenAIRE

    Sabino, João Filipe Moreira Lacerda

    2010-01-01

    O presente trabalho destina-se ao estudo do impacto da teoria constitucional dos tratados internacionais sobre direitos humanos na Convenção 158 da Organização Internacional do Trabalho (OIT). Sua importância se traduz na tentativa de relacionar parte da teoria constitucional dos direitos humanos ao direito do trabalho nacional, para a aplicação da normativa correta. Para tanto, inicia-se com a análise da conceituação e da denominação dos direitos humanos para melhor compreensã...

  2. Implementación de un Sistema de Gestión de Talento Humano para una Entidad Bancaria

    OpenAIRE

    Parrales Espinoza, Miguel Andres

    2015-01-01

    El proyecto tiene por objeto mejorarlos procesos administrativos del área de Recursos Humanos de la institución bancaria, mediante la implementación de un Sistema de Gestión de Talento Humano. En base a información obtenida, la institución bancaria utilizaba un Sistema de Administración de Recursos Humanos que cumplía con necesidades funcionales básicas para la gestión de personas. Es por ello que el proyecto implementado proporciona a la institución bancaria de un Sis...

  3. O conceito de fraternidade em Totalidade e Infinito e suas implicações para os direitos humanos

    OpenAIRE

    ELAND, Christopher James

    2016-01-01

    A presente dissertação tem como objetivo examinar a relação entre o conceito de fraternidade nos primeiros escritos de Emmanuel Levinas, especialmente em Totalidade e Infinito, e suas obras posteriores sobre Direitos Humanos a fim de investigar uma possível fundamentação não liberal para os Direitos Humanos na contemporaneidade. Para tanto, será necessário explicitar como a obra de Levinas sobre a relação ética para com o Outro que fundamenta sua ideia de Direitos Humanos se ancora na relação...

  4. Avance en el diseño de un péptido bloqueador del receptor Opioide Kappa 2 humano

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    Sonia Viviana Beltrán Velasquez

    2000-07-01

    Full Text Available Se evaluó la posibilidad de predecir una probable estructura secundaria para el Receptor Opioide Kappa 2 humano tomando como base la secuencia de aminoácidos del Receptor Opioide Kappa 1 humano. La estructura predicha mostró ser compatible con los datos que se poseen acerca de este tipo de receptores. Con esta prueba inicial, el proyecto que tiene como objetivo principal diseñar un análogo proteico para el Receptor Opioide Kappa 2 humano, ha mostrado el nivel mínimo de viabilidad necesario para ser continuado.

  5. AVANCE lEN IEL DISEÑO DIE UN PÉPTIDO BLOQUEADOR DIEL RECEPTOR OPIOIDIE KAPPA 2 HUMANO

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    Sonia Viviana Beltrán Velásquez

    2000-12-01

    Full Text Available Se evaluó la posibilidad de predecir una probable estructura secundaria para el Receptor Opioide Kappa 2 humano tomando como base la secuencia de aminoácidos del Receptor Opioide Kappa 1 humano. La estructura predicha mostró ser compatible con los datos que se poseen acerca de este tipo de receptores. Con esta prueba inicial, el proyecto que tiene como objetivo principal el diseñar un análogo proteico para el ReceptorOpioide Kappa 2 humano, ha mostrado el nivel mínimo de viabilidad necesario para ser continuado.

  6. Impacto de las reformas del sector de la salud sobre los recursos humanos y la gestión laboral

    OpenAIRE

    Pedro E. Brito Quintana

    2000-01-01

    Los recursos humanos, según sostienen quienes dirigen las iniciativas de reforma en el campo de la salud, constituyen el elemento clave de esas iniciativas y la principal ventaja competitiva que poseen los servicios de salud. Con la ayuda del Observatorio de los Recursos Humanos en las Reformas Sectoriales, los países han empezado por primera vez a recoger metódicamente pruebas de la importancia decisiva de los recursos humanos dentro de las iniciativas de reforma y, sobre todo, del impacto q...

  7. Del Recurso Humano al humano con recursos: una propuesta en el estudio de encadenamientos socioproductivos para el Turismo de intereses especiales en el territorio Patagonia Verde Chile

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    Guillermo Sebastián Pacheco Habert

    2012-01-01

    Full Text Available El tema del humano con recurso, enfoque de este trabajo, tiene un papel importantísimo a la hora de transitar de estrategias competitivas hacia estrategias colaborativas, sobre todo cuando se perciben logros ecosocioeconómicos que avanzan hacia la dirección de un desarrollo territorial. Se tiene como hipótesis, que desde el enfoque de los encadenamientos socioproductivos, centrado en el turismo de intereses especiales, es posible comprender y establecer objetivos comunes dentro de actores, organizaciones, emprendimientos y comunidades locales. El objetivo de este artículo es proponer una aproximación teórica sobre el TIE (Turismo de Intereses Especiales sobre las aglomeraciones, sobre los encadenamientos socioproductivos y sobre el enfoque del humano con recurso, para posteriormente relatar los primeros resultados de esta investigación realizada en los cinco municipios que hacen parte del territorio turístico denominado "Patagonia Verde", en el sur de Chile. A partir de la identificación de actores clave, se aplicaron entrevistas a éstos y fichas de caracterización a emprendimientos. Posteriormente se identificaron fortalezas y oportunidades que puedan ser potenciadas a partir de la perspectiva del humano con recurso. A partir de los encadenamientos socioproductivos, contar con individuos conscientes y proactivos dentro de las instituciones y organizaciones en tareas y ejes estratégicos del desarrollo turístico y que compartan con la perspectiva del desarrollo territorial sustentable, es uno de los desafíos que aquí proponemos. Son los sujetos quienes son capaces de generar las condiciones institucionales para otra forma de comprender y actuar en Turismo.

  8. Efecto de la Vitrificación de Ovocitos Humanos sobre la Capacidad de Unión y el Estado Acrosomal de Espermatozoides Humanos

    OpenAIRE

    Vilella, Ismael; García Hernández, Eva María; Ten Morro, Jorge; Bernabeu, Rafael; Segovia Huertas, Yolanda; Gómez-Torres, María José

    2015-01-01

    Conocer los aspectos moleculares que acontecen en el proceso de unión de los espermatozoides humanos a la zona pelúcida (ZP) humana es uno de los grandes retos de la biología de la Reproducción. Por otra parte conocer si el proceso de fecundación puede verse afectado por la criopreservación de los gametos femeninos sigue siendo otra cuestión debatida en la literatura. En base a esto, el objetivo principal de este trabajo fue conocer si la vitrificación ovocitaria puede alterar la interacción ...

  9. Las vestimentas que violan los derechos humanos de las mujeres: burka y velo islámico en la jurisprudencia de la Corte de Derechos Humanos de Europa.

    OpenAIRE

    Medina, Graciela

    2015-01-01

    En este artículo se examinan las legislaciones europeas en materia de uso del velo integral en espacios públicos, en particular, la ley francesa 1192/2010; y se estudia la jurisprudencia de la Corte de Derechos Humanos de Europa sobre la materia, en concreto, el reciente caso 'S.A.S contra Francia'. This paper analyzes the European legislation regarding the use of the full veil in public spaces, specially the French law 1192/2010, and the jurisprudence of the European Court of Hum...

  10. Os direitos humanos aplicados à fiscalização tributária: sigilo fiscal e ponderação dos direitos humanos fundamentais

    OpenAIRE

    Guimarães, Adriana Esteves

    2013-01-01

    Este estudo considera a supranacionalidade via inovadora e indispensável para maximizar a efetividade dos direitos humanos em razão da fiscalização tributária. O direito de fiscalização do Estado encontra limites nos direitos e garantias fundamentais do cidadão. A privacidade e o sigilo de informações, sejam elas bancárias ou fiscais, bem como o direito ao devido processo legal, contraditório e ampla defesa celebram a oportunidade garantida ao cidadão comum para exercer sua ...

  11. Estudio de la asimetría interhemisférica en la corteza prefrontal dorsolateral de sujetos humanos

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    Hernán J. Pimienta J.

    2005-03-01

    Full Text Available Se presentan los resultados de un trabajo de investigación encaminado a establecer las diferencias entre los hemisferios cerebrales de la corteza prefrontal dorsolateral: áreas 46 laeral y 7 medial, de sujetos humanos.

  12. As raízes renegadas da teoria do capital humano

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    Saul Renato P.

    2004-01-01

    Full Text Available O artigo pretende reconstituir a trajetória da teoria do capital humano, no contexto da sua sociedade de origem, os Estados Unidos. A partir da identificação de tendências presentes no pensamento social americano, marcadamente do orientado, de início, para a organização do trabalho industrial através das experiências levadas a efeito por Frederick Taylor e, depois, pela teorização a respeito de um novo conceito de propriedade emergente no processo de organização das grandes empresas de capital acionário, enquadra-se a teoria do capital humano no campo de resolução do grande dilema do liberalismo americano, o da institucionalização de uma sociedade sem classes.

  13. El crecimiento endógeno a partir de las externalidades del capital humano

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    Gaviria Ríos Mario Alberto

    2007-08-01

    Full Text Available Partiendo del enfoque de Robert Lucas (1988, este trabajo desarrolla un
    modelo de crecimiento endógeno que considera dos efectos de la acumulación
    de capital humano: el primero de orden interno, que mide el impacto
    sobre la productividad de cada persona y el segundo de orden externo,
    relacionado con la contribución a la productividad de los demás factores
    de producción. Para los propósitos del ejercicio, la propuesta de Lucas
    se complementa con la descomposición del factor tecnológico en dos componentes,
    uno exógeno y otro endógeno, de acuerdo a las interacciones
    entre el capital humano y el cambio técnico.

  14. Gestão de recursos humanos no Centro Distrital de Aveiro, ISS, I.P.

    OpenAIRE

    Gouveia, Carla Filipa Ferreira

    2013-01-01

    Este relatório de estágio surge no âmbito do estágio curricular no Núcleo de Recursos Humanos do Centro Distrital da Segurança Social de Aveiro, no contexto curricular do mestrado em Administração e Gestão Pública. Para além da descrição das atividades realizadas em ambientes de estágio, é ainda necessário um enquadramento teórico sobre as práticas de gestão de recursos humanos, principalmente no que se refere à necessidade de formação profissional e a aplicação de sistemas de avaliação de...

  15. Agua y desarrollo humano en el Estado de México

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    José Luis Montesillo-Cedillo

    2011-01-01

    Full Text Available El objetivo del presente trabajo es analizar la relación entre el nivel de precipitación pluvial medio anual en los 125 municipios que conforman al Estado de México, el índice de desarrollo humano (IDH y los componentes del índice de marginación. El principal resultado es que la disponibilidad natural de agua no tiene relación con el IDH y que su relación con los componentes del índice de marginación es negativa, aunque poco. En consecuencia, se concluye que la disponibilidad natural de agua no es factor de desarrollo humano ni de desarrollo económico en los municipios del Estado de México.

  16. Los derechos humanos en la política de seguridad democrática

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    Orlando José Cadrazco Salcedo

    2010-07-01

    Full Text Available ResumenAfirmar, por tanto, que los seres humanos tienen derechos humanos significa, no que los seres humanos los tienen reconocidos en un ordenamiento jurídico-positivo dado -el colombiano, el finlandés o el de la Organización de Naciones Unidas- determinados derechos, ni que éstos están plenamente protegidos y garantizados por los diferentes operadores jurídicos, ni siquiera que los seres humanos disfrutan de los mismos, sino que en un sistema normativo ideal -que se considera en buena lógica plenamente justificado- los individuos cuentan con esos derechos humanos; y significa también que es deseable que los ordenamientos jurídicos positivos -los diferentes Derechos nacionales y el Derecho Internacional- se acerquen a ese sistema normativo ideal, los reconozcan positivamente y garanticen, por tanto, esos derechos a los ciudadanos. No es un sin sentido afirmar que todos los seres humanos tienen derecho a la vida, a la libertad de expresión, a la salud etc., aunque desgraciadamente muchos de ellos vivan en países totalitarios donde esos -y otros- derechos humanos no han sido recogidos en los textos jurídico-positivos, o aún viviendo en países democráticos, permanezcan en la más absoluta indigencia, y no disfruten -por tanto- realmente de ellosPalabras clave.Derechos Humanos, Seguridad Democrática, Políticas Públicas, Democracia, convivencia. AbstractState, therefore, that human beings have human rights means that human beings do not have a recognized legal system-as-good Colombian, Finnish ... or the United Nations-specific rights, or that they are fully protected and guaranteed by different legal operators, or even that humans enjoy the same, but in an ideal regulatory system is considered in good logic fully justified these individuals have human rights, and also means that it is desirable that the positive-legal systems different national laws and international law that is closer to the ideal

  17. LA GERENCIA DEL TALENTO HUMANO BAJO LA PERSPECTIVA DE LA CONDICIÓN HUMANA

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    OMAR CABRALES SALAZAR

    2009-01-01

    Full Text Available El artículo destaca la importancia de los atributos de la condición humana y del desarrollo del potencial humano en la optimización de las competencias de los gerentes de hoy. Se plantea que con el propósito de mejorar la gestión del talento humano y poder comprender y gestionar mejor a su equipo de trabajo, el gerente debe conocer cuáles son los atributos de la condición humana y cómo desarrollar las competencias para trabajarlos con optimismo, de manera tal que implemente estrategias para la mejora del talento de su gente. En este marco el talento se observa como una variable inagotable y susceptible de permanente desarrollo. El documento concluye haciendo un llamado al reconocimiento de la condición humana en las organizaciones, como estrategia necesaria para afrontar las inequidades de la sociedad actual.

  18. ¿Mejora el bono de desarrollo humano la lactancia materna exclusiva en Ecuador?

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    César Carranza Barona

    2015-01-01

    Full Text Available En este artículo se evalúa el impacto del Bono de Desarrollo Humano en Ecuador sobre la adopción de prácticas saludables de ali - mentación y nutrición, específicamente en el cumplimiento de la norma internacional sobre lactancia materna exclusiva propuesta por la organización Mundial de la Salud ( OMS . Mediante la técnica econométrica de evaluación de impacto, denominada regresión discontinua difusa, se encontró que el Bono de Desarrollo Humano no tiene impacto significativo en la adopción de esta norma por parte de sus beneficiarios

  19. Prazer e sofrimento no trabalho: representações sociais de profissionais de recursos humanos

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    Roque Tadeu Gui

    Full Text Available Este estudo descreve as representações sociais de profissionais da área de Recursos Humanos do Banco do Brasil sobre prazer e sofrimento no trabalho. A pesquisa tem fundamentação teórica na Psicodinâmica do Trabalho e na Teoria das Representações Sociais. A metodologia adotada é de orientação qualitativa, utilizando-se a técnica do grupo focal como principal meio de coleta dos dados. Os participantes da pesquisa reconhecem a existência de situações que geram prazer e sofrimento no Banco do Brasil e admitem que é papel dos profissionais de Recursos Humanos intervir nas situações de sofrimento, com o objetivo de diminuí-lo e de potencializar as vivências de prazer na Organização.

  20. Gestion de recursos humanos en una universidad con un enfoque sistemico

    OpenAIRE

    Sarapura Yupanqui, Kruger Teodomiro; Sarapura Yupanqui, Kruger Teodomiro

    1998-01-01

    Los recursos humanos de las organizaciones representan a una de sus mayores fortalezas en potencia. Sin embargo, todavía no se aprovecha en el desarrollo de las mismas; más aún en las universidades que también tienen esta característica. Por lo que una gestión de recursos humanos en la universidad con un enfoque sistémico permitirá al sistema universitario lograr una ventaja competitiva. Para ello, se hace el análisis, el diseño y la construcción de un modelo de esta gestión y en resumen cons...