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Sample records for medicine semen armeniacae

  1. Evolutionary Medicine: Semen Sampling and Seminal Plasma Hypersensitivity

    Directory of Open Access Journals (Sweden)

    Gordon G. Gallup

    2014-01-01

    Full Text Available Evidence suggests that each male may have a unique semen signature, and there are reasons to consider the possibility that semen sampling (i.e., being inseminated by different prospective mates during courtship may be part of an evolved female mate assessment strategy. Here we theorize that seminal plasma hypersensitivity represents the extreme negative end of this continuum and functions as a deterrent to mating with genetically incompatible suitors.

  2. Bioactivity-guided fractionation identifies amygdalin as a potent neurotrophic agent from herbal medicine Semen Persicae extract.

    Science.gov (United States)

    Yang, Chuanbin; Zhao, Jia; Cheng, Yuanyuan; Li, Xuechen; Rong, Jianhui

    2014-01-01

    Herbal medicine Semen Persicae is widely used to treat blood stasis in Chinese medicine and other oriental folk medicines. Although little is known about the effects of Semen Persicae and its active compounds on neuron differentiation, our pilot study showed that Semen Persicae extract promoted neurite outgrowth in rat dopaminergic PC12 cells. In the present study, we developed a bioactivity-guided fractionation procedure for the characterization of the neurotrophic activity of Semen Persicae extract. The resultant fractions were assayed for neurite outgrowth in PC12 cells based on microscopic assessment. Through liquid-liquid extraction and reverse phase HPLC separation, a botanical glycoside amygdalin was isolated as the active compound responsible for the neurotrophic activity of Semen Persicae extract. Moreover, we found that amygdalin rapidly induced the activation of extracellular-signal-regulated kinase 1/2 (ERK1/2). A specific ERK1/2 inhibitor PD98059 attenuated the stimulatory effect of amygdalin on neurite outgrowth. Taken together, amygdalin was identified as a potent neurotrophic agent from Semen Persicae extract through a bioactivity-guided fractional procedure. The neurotrophic activity of amygdalin may be mediated by the activation of ERK1/2 pathway.

  3. Bioactivity-Guided Fractionation Identifies Amygdalin as a Potent Neurotrophic Agent from Herbal Medicine Semen Persicae Extract

    Directory of Open Access Journals (Sweden)

    Chuanbin Yang

    2014-01-01

    Full Text Available Herbal medicine Semen Persicae is widely used to treat blood stasis in Chinese medicine and other oriental folk medicines. Although little is known about the effects of Semen Persicae and its active compounds on neuron differentiation, our pilot study showed that Semen Persicae extract promoted neurite outgrowth in rat dopaminergic PC12 cells. In the present study, we developed a bioactivity-guided fractionation procedure for the characterization of the neurotrophic activity of Semen Persicae extract. The resultant fractions were assayed for neurite outgrowth in PC12 cells based on microscopic assessment. Through liquid-liquid extraction and reverse phase HPLC separation, a botanical glycoside amygdalin was isolated as the active compound responsible for the neurotrophic activity of Semen Persicae extract. Moreover, we found that amygdalin rapidly induced the activation of extracellular-signal-regulated kinase 1/2 (ERK1/2. A specific ERK1/2 inhibitor PD98059 attenuated the stimulatory effect of amygdalin on neurite outgrowth. Taken together, amygdalin was identified as a potent neurotrophic agent from Semen Persicae extract through a bioactivity-guided fractional procedure. The neurotrophic activity of amygdalin may be mediated by the activation of ERK1/2 pathway.

  4. What do Male Students at the College of Medicine of the University of Malawi Say About Semen Donation?

    Directory of Open Access Journals (Sweden)

    Fanuel Lampiao

    2013-02-01

    Full Text Available Aim This study was aimed at assessing knowledge and attitude to semen donation among male students at the College of Medicine. Methods A semi-structured questionnaire survey of students doing Pre-Medical Sciences, MBBS, Pharmacy, Physiotherapy, and Medical Laboratory Science degrees at the College of Medicine. There were 180 respondents who took part in this study. Their ages ranged from 15 to 43 years with a mean of 21.21 years. Results A total of 130 (72.2% of the respondents were aware of the practice of sperm donation for research purposes or the treatment of infertility while 50 students had never heard of it. A total of 86 (47.8% students reported their willingness to donate their semen. The main motivation for wanting to donate sperm was to get paid. The leading factors which discouraged the respondents from donating semen were that the practice was either against their religious belief (42.6%, or that they were not comfortable with the practice of semen donation because it was morally wrong (50%. Conclusion Despite having the high level of awareness of semen donation among male students of the College of Medicine, more than half of them were unfavourably disposed to it. Public enlightenment through the mass media and correction of false notions about semen donation will go a long way in addressing this problem. [TAF Prev Med Bull 2013; 12(1.000: 75-78

  5. [Influence on Strychni Semen's Analgesic Effect and Toxicity of Milk-Impregnated- Processing-Technology of Traditional Uighur Medicine].

    Science.gov (United States)

    Tursun, D; Shi, Yu-zhu; Yang, Qiao-li; Liu, Yan; Xue, Wen-cai; Abas, A; Yang, Wei-jun

    2015-02-01

    To investigate the effects of different processing methods on analgesic effect and toxicity of Strychni Semen, and to provide the scientific basis of milk-impregnated-processing-technology of traditional Uighur medicine for toxic medicines. Three different methods, frying with sand of traditional Chinese medicine (1), cooled milk-impregnated-processing-technology of traditional Uighur medicine (2), and heated milk-impregnated-processing-technology of traditional Uighur medicine (3) were used to process raw Strychni Semen and the processed products were compared with those in the raw drug (4). Karber method was used to caculate the LD50. The LD50 of (4), (1), (2) and (3)were 273.0, 289.3, 289.3 and 339.2 mg/kg,successively. Low dose group of heated milk-impregnated-processing-technology, low and high dose groups of cooled milk-impregnated-processing-technology had significant inhibition on the acetic acid-induced writhing reaction in mice (P effect in mice while improving the LD50 of mouse, which illustrates milk-impregnated-processing- technology's pharmacological mechanism on efficacy enhancing and toxicity reducing of Strychni Semen.

  6. The Therapeutic Effects of a Medicinal Plant Mixture in Capsule Form on Catalase Levels in the Semen of Men with Oligospermia

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    Alizadeh Hasti

    2015-01-01

    Full Text Available Objective: In the present study, the therapeutic effects of mixed herbs (onion, ginger, basil, cinnamon, orange peel, yellow and red watermelon seeds, and carrot seed on catalase levels in the semen of men with oligospermia were evaluated. About 50% of recognized infertility factors are male-related factors, and are mainly the result of oligospermia, astenospermia, and teratozoospermia. Materials and Methods: The study participants included 40 males with oligospermia and infertility. The studied medicine were 700 mg capsules containing onion, ginger, basil, cinnamon, orange peel, yellow and red watermelon seeds, and carrot seed (100 mg of each. Catalase activity was measured by Aebi method. Results: A significant increase was observed in catalase level in semen as a result of using the medicinal plant mixture (P < 0.001. Conclusion: Free radicals play an important role in male infertility. Antioxidants can prevent the damaging effects they have on sperm. Oxidative stress reduction can increase the chances of natural fertility or assisted reproductive technology (ART. Medicinal plants have low costs, complications, and easy availability, and cause an increase in semen plasma antioxidants and subsequent improvement in semen parameters. Thus, they can be the source of new hopes for the treatment of infertility.

  7. Semen analysis

    Science.gov (United States)

    ... sperm in the semen. This can confirm the success of the vasectomy. The test may also be ... ADAM Health Solutions. About MedlinePlus Site Map FAQs Customer Support Get email updates Subscribe to RSS Follow ...

  8. [Optimization for supercritical CO2 extraction with response surface methodology of Prunus armeniaca oil].

    Science.gov (United States)

    Chen, Fei-Fei; Wu, Yan; Ge, Fa-Huan

    2012-03-01

    To optimize the extraction conditions of Prunus armeniaca oil by Supercritical CO2 extraction and identify its components by GC-MS. Optimized of SFE-CO extraction by response surface methodology and used GC-MS to analysis Prunus armeniaca oil compounds. Established the model of an equation for the extraction rate of Prunus armeniaca oil by supercritical CO2 extraction, and the optimal parameters for the supercritical CO2 extraction determined by the equation were: the extraction pressure was 27 MPa, temperature was 39 degrees C, the extraction rate of Prunus armeniaca oil was 44.5%. 16 main compounds of Prunus armeniaca oil extracted by supercritical CO2 were identified by GC-MS, unsaturated fatty acids were 92.6%. This process is simple, and can be used for the extraction of Prunus armeniaca oil.

  9. Prospective Analysis on the Effect of Botanical Medicine (Tribulus terrestris) on Serum Testosterone Level and Semen Parameters in Males with Unexplained Infertility.

    Science.gov (United States)

    Roaiah, Mohamed Farid; Elkhayat, Yasser Ibrahim; Saleh, Sameh Fayek GamalEl Din; Abd El Salam, Mohamed Ahmed

    2016-06-23

    We evaluated the role of Tribulus terrestris in males with unexplained infertility and its effect on serum testosterone and semen parameters. Thirty randomized male patients presenting to Andrology outpatient clinic complaining of idiopathic infertility were selected. They were given Tribulus terrestris (750 mg) in three divided doses for three months. The effect of Tribulus terrestris on serum testosterone (total and free) and luteinizing hormone (LH), as well as its impact on semen parameters in those patients, was studied. No statistically significant difference was observed in the levels of testosterone (total and free) and LH and semen parameters (sperm concentration or motility, or abnormal forms) before and after the treatment. In addition, no statistically significant correlations were observed between testosterone (free and total) and LH and semen parameters before and after the treatment. Tribulus terrestris was ineffective in the treatment of idiopathic infertility.

  10. Cryopreservation of crane semen

    Science.gov (United States)

    Gee, G.F.; Harris, James

    1991-01-01

    The method for the cryopreservation of crane semen at Patuxent Wildlife Research Center is described in detail. Cryopreservation is useful for the long-term storage of crane semen and for specialized propagation needs. A 50% fertility rate from most sandhill cranes, Grus canadensis, inseminated with frozen-thawed semen can be expected. Additional research should improve the fertility rate and determine how applicable the technique is to other crane species.

  11. Cryopreservation of mammalian semen.

    Science.gov (United States)

    Curry, Mark R

    2007-01-01

    Mammalian spermatozoa were among the very first cells to be successfully cryopreserved and over the last five decades the use of frozen-thawed semen for artificial insemination has come to play an important role in domestic livestock production. More recently, semen freezing has increasingly been utilized in the establishment of genetic resource banks for endangered species. Semen is collected, most commonly either by use of an artificial vagina or by electroejaculation of an anaesthetized animal, and basic sperm parameters assessed. Semen is extended using a TEST-egg yolk-glycerol diluent, packaged in 0.25-mL plastic straws and slowly cooled to 5 degrees C over a period of 1-2 h. Cooled straws are frozen by suspending within liquid nitrogen vapor above the liquid nitrogen surface before plunging into the liquid phase. Straws are thawed briefly in air before immersing in a 35 degrees C water bath for 15 s, and often are used directly for insemination without any further processing.

  12. The Issues of Apricot (Prunus Armeniaca L. Micropropagation

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    Martina Kudělková

    2017-01-01

    Full Text Available The effect of four modified mediums for apricot multiplication was observed in this study. A total number of 1864 single nodes of 20 Prunus armeniaca L.varieties were established. Explants surface was disinfected with 0.2 % mercuric chloride for 5 minutes. MS (1962 medium with 0.5 mg.l−1 BA, 0.01 mg.l−1 NAA and 0.5 mg.l−1 GA3 was used as a medium for primary culture. ‘Velkopavlovická’, ‘Bergeron’, genotype 1128 and genotype LE 2927 Š9 were successfully transferred to aseptic conditions and multiplied. Modified MS medium (1962, DKW/Juglans medium, Quoirin, Lepoivre (1977 medium and Marino et al. (1991 medium were used for multiplication. Modified MS medium and modified DKW/Juglans medium were not suitable for apricot multiplication at all and explants did not grow. The best results were observed in the case of Quoirin, Lepoivre (1977 medium with 0.4 mg.l−1 BA and 0.01 mg.l−1 NAA. Young plants multiplied well, were fresh and vital and no damage was observed. The highest number of new shoots was observed in the case of Marino et al. (1991 medium. The average growth of new shoots after the last passaging was 600 %, rate 7.33 (Velkopavlovická; 566 %, rate 7.0 (Bergeron; 475 %, rate 6.25 (1128 and 483 %, rate 6.33 (LE 2927 Š9. However, new shoots in clusters were too dense and stunted and this medium is not recommended for apricot multiplication.

  13. Semen analysis and prediction of natural conception

    NARCIS (Netherlands)

    Leushuis, Esther; van der Steeg, Jan Willem; Steures, Pieternel; Repping, Sjoerd; Bossuyt, Patrick M. M.; Mol, Ben Willem J.; Hompes, Peter G. A.; van der Veen, Fulco

    2014-01-01

    Do two semen analyses predict natural conception better than a single semen analysis and will adding the results of repeated semen analyses to a prediction model for natural pregnancy improve predictions? A second semen analysis does not add helpful information for predicting natural conception

  14. Semen and the diagnosis of infertility in Aristotle.

    Science.gov (United States)

    Trompoukis, C; Kalaitzis, C; Giannakopoulos, S; Sofikitis, N; Touloupidis, S

    2007-02-01

    Aristotle (384-322bc) was one of the leading intellectual figures of all time. In his work he systematised a massive amount of knowledge on a diverse range of subjects, including medicine. This article discusses the observations and hypotheses of this great philosopher on semen and infertility, as they are presented in his work Generation of Animals. This is combined with an evaluation of his positions in relation to those of the Hippocratic Corpus on the same subject. An extensive review of Aristotle's work Generation of Animals was performed with particular focus on his perspectives about semen and infertility. Publications referring to this work were also reviewed. According to Aristotle, semen is that which contains the principles that come from both parents when they unite. He believed that semen was formed by the secretion of nutriments by the body, developing his theories of sterility on this basic principle. A lack of fertility is attributed to genetic or acquired causes. He proposed methods for diagnosing sterility, primarily the 'water test' for men and the 'pessary' method for women. Even if his observations contain clear mistakes, such as attributing only secondary functions to male testicles and the identification of menses as women's 'seed', Aristotle's views also contain keen observations and exceptional thinking, both on the characteristics of semen and the causes of sterility (infertility).

  15. Association between use of phthalate-containing medication and semen quality among men in couples referred for assisted reproduction

    DEFF Research Database (Denmark)

    Broe, Anne; Pottegård, A; Hallas, Jesper

    2018-01-01

    studies. One study has shown associations between medicinal phthalate exposure and poor semen quality, suggesting similar effects in humans. STUDY DESIGN, SIZE, DURATION: We included 18,515 males with poor semen quality (cases) and 31,063 males with normal semen quality (controls) registered in the Danish...... AND THE ROLE OF CHANCE: In total, 57 cases and 72 controls redeemed at least one prescription for a drug containing ortho-phthalates in the 90 days before their first semen sample, yielding an adjusted odds ratio (OR) of 1.30 (95% CI: 0.91-1.85) for poor semen quality when compared to males exposed...... to phthalate-free generic drugs. Similarly, 81 cases and 78 controls exposed to a drug containing polymers had increased odds of poor semen quality (OR=1.71, 95% CI: 1.24-2.35). Current exposure to polymer containing products from alimentary tract and metabolism drugs was associated with the highest OR of 2...

  16. Colloid centrifugation of boar semen.

    Science.gov (United States)

    Morrell, J M; Wallgren, M

    2011-09-01

    Colloid centrifugation of boar semen has been reported sporadically for at least the last two decades, beginning with density gradient centrifugation (DGC) and progressing more recently to single layer centrifugation (SLC). Single layer centrifugation through a species-specific colloid has been shown to be effective in selecting the best spermatozoa (spermatozoa with good motility and normal morphology) from boar sperm samples. The method is easier to use and less time-consuming than DGC and has been scaled-up to allow whole ejaculates from other species, e.g. stallions, to be processed in a practical manner. The SLC technique is described, and various scale-up versions are presented. The potential applications for SLC in boar semen preservation are as follows: to improve sperm quality in artificial insemination (AI) doses for 'problem' boars; to increase the shelf-life of normal stored sperm samples, either by processing the fresh semen before preparing AI doses or by processing the stored semen dose to extract the best spermatozoa; to remove pathogens (viruses, bacteria), thus improving biosecurity of semen doses and potentially reducing the use of antibiotics; to improve cryosurvival by removing dead and dying spermatozoa prior to cryopreservation; to select spermatozoa for in vitro fertilization. These applications are discussed and practical examples are provided. Finally, a few thoughts about the economic value of the technique to the boar semen industry are presented. © 2011 Blackwell Verlag GmbH.

  17. Cryopreservation of American kestrel semen with dimethylsulfoxide

    Science.gov (United States)

    Gee, G.F.; Morrell, C.A.; Franson, J. Christian; Pattee, Oliver H.

    1993-01-01

    Semen samples from 15 male American Kestrels (Falco sparverius) were frozen in dimethyl sulfoxide (DMSO). The semen was thawed 1-14 mo later and used to inseminate six females during three breeding seasons. Kestrels inseminated with thawed semen containing 4% DMSO produced only infertile eggs (N = 14). Kestrels inseminated with thawed semen containing 6%, 8%, or 10% DMSO produced fertile eggs (N = 14) and live chicks (N = 6). Progressive motility of spermatozoa in thawed semen containing 10% DMSO was less (44 ? 6%) than in thawed semen containing 6% (62 ? 10%) or 8% (61 ? 1%) DMSO.

  18. 9 CFR 98.36 - Animal semen from Canada.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Animal semen from Canada. 98.36... CERTAIN ANIMAL EMBRYOS AND ANIMAL SEMEN Certain Animal Semen § 98.36 Animal semen from Canada. (a) General importation requirements for animal semen from Canada. If the product is . . . Then . . . (1) Equine semen...

  19. Semenic Mountains’ alpine skiing area

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    Petru BANIAȘ

    2017-03-01

    Full Text Available The present paper presents, after a short history of alpine skiing which describes apparition, necessity, utility and universality of skiing during time, a comparative study referring to the alpine skiing domain in the Semenic Mountains area. In the paper are also presented general notions about alpine skiing methodology together with an ample description of the plateau area form Semenic Mountains, describing localization and touristic potential. Based on the SWOT analysis made for each slope, was realized a complex analysis of the entire skiing domain, an analysis which includes technical, financial, climatic and environmental aspects, along with an analysis of the marketing policy applied for the specific zone.

  20. Viability of bull semen extended with commercial semen extender ...

    African Journals Online (AJOL)

    After macroscopic evaluation, semen was pooled and aliquoted randomly into Triladyl, modified Ham's F10, and TCM-199 culture media, and then stored at 24 °C. Sperm motility parameters, morphology, and viability were analysed with computer aided sperm analysis (CASA) after 0, 24, 48 and 72 hours. The study was ...

  1. semen by MTT reduction assay

    African Journals Online (AJOL)

    user

    the concentration of sperm in each semen sample, sperm motility, plasma integrity of sperm in terms of hypo-osmotic swelling (HOS) test, live and dead ratio of sperm and MTT reduction assay of each ejaculate were determined. Plasma membrane integrity of fresh sperm was assessed using a hypo-osmotic swelling (HOS) ...

  2. Physical activity is not related to semen quality in young healthy men.

    Science.gov (United States)

    Mínguez-Alarcón, Lidia; Chavarro, Jorge E; Mendiola, Jaime; Gaskins, Audrey J; Torres-Cantero, Alberto M

    2014-10-01

    To study the relationship of physical activity with semen quality among healthy young men from Spain. Cross-sectional study. University and college campuses of Murcia Region, Spain. Healthy young men with untested fertility (n = 215). A physical examination, blood and semen samples, and completion of a questionnaire. Semen quality parameters. Physical activity was not related to semen quality parameters. The adjusted percentage differences (95% confidence interval) in semen parameters comparing men in the top quartile of moderate-to-vigorous physical activity (≥9.5 h/wk) with men in the bottom quartile (≤3 h/wk) were 4.3% (-30.2%, 38.9%) for total sperm count, 7.2% (-30.6%, 45.1%) for sperm concentration, -2.42% (-6.53%, 1.69%) for sperm motility, and 12.6% (-12.0%, 37.2%) for sperm morphology. In contrast to previous research among athletes, these data suggest that physical activity is not deleterious to testicular function, as captured by semen quality parameters in this population of healthy young men in Spain. Copyright © 2014 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

  3. Reactive oxygen species in human semen: validation and qualification of a chemiluminescence assay.

    Science.gov (United States)

    Vessey, Wayne; Perez-Miranda, Ana; Macfarquhar, Rachel; Agarwal, Ashok; Homa, Sheryl

    2014-12-01

    To standardize and validate an assay for reactive oxygen species (ROS) in human semen. ROS levels assayed in blanks, negative and positive control samples (30% H2O2), and human semen, with the use of a luminol-based chemiluminescence assay measured in a single tube luminometer. Andrology laboratory. Semen samples from 19 men attending for routine semen analysis. None. ROS levels reported in relative light units (RLU) per second, adjusted for sperm concentration. The ROS assay equipment performed according to expectations, generating a chemiluminescence signal for positive control samples and semen samples that decayed rapidly and was captured within 10 minutes. Blanks and negative control samples gave negligible readings. There was no significant intra- or interassay variation. Interference from extraneous factors was negligible. The assay distinguished changes in ROS over a wide range of concentrations and provided consistent results between reagent batches. Working reagents remained stable for 3 months. Acceptable levels for negative and positive control samples were established to set criteria for the test passing or failing on any given day. The assay was sensitive to ambient temperature >25°C. ROS declined significantly with time after ejaculation. Mechanical agitation doubled ROS production in semen. These results validate the ROS assay and demonstrate that it is a highly reliable and accurate diagnostic test. Copyright © 2014 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

  4. Environmental factors and semen quality

    DEFF Research Database (Denmark)

    Jurewicz, Joanna; Hanke, Wojciech; Radwan, Michal

    2009-01-01

    OBJECTIVES: An increasing number of reports suggest that chemical and physical agents in the environment, introduced and spread by human activity, may affect male fertility in humans. This article aims at evaluating the impact of environmental exposures (pesticides, phthalates, PCBs, air pollution...... to sperm motility. In case of air pollution, studies suggest a link between ambient air pollutants and various semen characteristics. Additional research is needed to corroborate this association and to establish the causal agents. Results of few studies on subfertile men demonstrate associations between...... phthalate levels commonly experienced by the public and impaired sperm quality (impact on sperm concentration, morphology, motility), but the findings have not been corroborated in studies of men from the general population. Mobile phones might adversely affect the quality of semen by decreasing mostly...

  5. Semen parameters in polyzoospermic men

    Directory of Open Access Journals (Sweden)

    S. Sh. Khayat

    2015-01-01

    Full Text Available Polyzoospermia is extremely high (above 250 mln/ml sperm concentration with normal volume of ejaculate. In the laboratory of genetic disorders of reproduction of Research Centre for Medical Genetics in 2007–2012 years we analyzed more than 14 000 semen samples from infertile men and men with reproductive disorders in order to study the distribution and characteristics of spermatogenesis in polyzoospermic men. Only polyzoospermic men semen analysis results were interpretered in present article. Polyzoospermia (sperm count over 250 mln/ml in volume over 1,5 ml was detected in 191 semen samples (1.3 %. At the same time 15 % of the samples with polyzoospermia were normozoospermic. Among 85 % of the sperm pathology samples asthenozoospermia occured most frequently (77 %, astenoteratozoospermia was detected in 8 % of cases with polyzoospermia. The average proportion of vitality was 90.09 ± 10.02 %, normal morphology – 14.93 ± 8.51 %, and progressive motility – 17.72 ± 11.81 %. The average concentration of spermatozoa in the ejaculate in the examined samples was 313.29 ± 64.78 mln/ml. More than half of the tested samples with polyzoospermia had a concentration 250–300 mln/ml. Concentration of 450 mln/ml and more detected in 3 % of samples. The maximum total number of spermatozoa in the ejaculate in our study was more than 2 billion sperm cells and was observed in two polyzoospermic men. One of these patients had the maximum concentration (615 mln/ml also. We found a high correlation (r = 0.89; p < 0.01 between the volume of ejaculate and the total number of spermatozoa. Immature germ cells from ejaculate were estimated in 7 patients. There was a partial spermatogenesis arrest at meiosis I prophase in 5 out 7 of the examined semen samples. 

  6. Effects of work and life stress on semen quality.

    Science.gov (United States)

    Janevic, Teresa; Kahn, Linda G; Landsbergis, Paul; Cirillo, Piera M; Cohn, Barbara A; Liu, Xinhua; Factor-Litvak, Pam

    2014-08-01

    To evaluate associations between work-related stress, stressful life events, and perceived stress and semen quality. Cross-sectional analysis. Northern California. 193 men from the Child Health and Development Studies evaluated between 2005-2008. None. Measures of stress including job strain, perceived stress, and stressful life events; outcome measures of sperm concentration, percentage of motile sperm, and percentage of morphologically normal sperm. We found an inverse association between perceived stress score and sperm concentration (estimated coefficient b=-0.09×10(3)/mL; 95% confidence interval [CI]=-0.18, -0.01), motility (b=-0.39; 95% CI=-0.79, 0.01), and morphology (b=-0.14; 95% CI, -0.25, -0.04) in covariate-adjusted linear regression analyses. Men who experienced two or more stressful life events in the past year compared with no stressful events had a lower percentage of motile sperm (b=-8.22; 95% CI, -14.31, -2.13) and a lower percentage of morphologically normal sperm (b=-1.66; 95% CI, -3.35, 0.03) but a similar sperm concentration. Job strain was not associated with semen parameters. In this first study to examine all three domains of stress, perceived stress and stressful life events but not work-related stress were associated with semen quality. Copyright © 2014 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

  7. Stainless steel welding and semen quality

    DEFF Research Database (Denmark)

    Jelnes, J E; Knudsen, Lisbeth E.

    1988-01-01

    Questionnaire studies of patients from fertility clinics suggest that welders may have an increased risk of reduced semen quality. In this study, welders and nonwelders from the same plants were asked to provide blood, urine, and semen samples. Urine was analyzed for chromium and nickel, and for ...

  8. Relation between semen quality and fertility

    DEFF Research Database (Denmark)

    Bonde, Jens Peter; Ernst, Erik; Jensen, Tina Kold

    1998-01-01

    Semen analysis is part of the routine assessment of infertile couples. WHO defines a sperm concentration above 20x10(6) per mL seminal fluid as normal. We studied the association between semen quality and the probability of conception in a single menstrual cycle in Danish couples with no previous...

  9. Ebola Virus Persistence in Semen Ex Vivo.

    Science.gov (United States)

    Fischer, Robert J; Judson, Seth; Miazgowicz, Kerri; Bushmaker, Trent; Munster, Vincent J

    2016-02-01

    On March 20, 2015, a case of Ebola virus disease was identified in Liberia that most likely was transmitted through sexual contact. We assessed the efficiency of detecting Ebola virus in semen samples by molecular diagnostics and the stability of Ebola virus in ex vivo semen under simulated tropical conditions.

  10. Study on Fatty Constituents in Coicis Semen

    OpenAIRE

    田, 瑞華; 丁, 怡; 野原, 稔弘; 高井, 一也; 滝口, 靖憲; 大塚, 英明; 山崎, 和男; RUI-HUA, TIAN; YI, DING; TOSHIHIRO, NOHARA; KAZUYA, TAKAI; YASUNORI, TAKIGUCHI; HIDEAKI, OHTUKA; KAZUO, YAMASAKI; 熊本大学薬学部

    1997-01-01

    From Coicis Semen, seeds of Coix lachryma-jobi var. ma-yuen, usual seven triglycerides, three fatty acids and a phospholipid were isolated but no coixenolide. We confirmed that any substance corresponding to coixenolide was not present in an extract of Coicis Semen by using synthesized coixenolide as reference.

  11. Stainless steel welding and semen quality

    DEFF Research Database (Denmark)

    Jelnes, J E; Knudsen, Lisbeth E.

    1988-01-01

    Questionnaire studies of patients from fertility clinics suggest that welders may have an increased risk of reduced semen quality. In this study, welders and nonwelders from the same plants were asked to provide blood, urine, and semen samples. Urine was analyzed for chromium and nickel, and for ......Questionnaire studies of patients from fertility clinics suggest that welders may have an increased risk of reduced semen quality. In this study, welders and nonwelders from the same plants were asked to provide blood, urine, and semen samples. Urine was analyzed for chromium and nickel...... and nonwelders. Because the metal dust exposure of nonwelders in the plant may be higher than that in the general population, welders were also compared to referents not working in the metal industry. Again, no decrease in semen quality associated with welding was demonstrated....

  12. Poor Semen Quality Predicts Increased Mortality

    DEFF Research Database (Denmark)

    Jensen, Tina Kold; Bostofte, Erik; Jacobsen, Rune

    is not increased. The long-term survival of men with poor semen quality is, however, unknown. We therefore studied the associations between semen characteristics and subsequent mortality. Back to Top Material and Methods: The Copenhagen Sperm Analysis Laboratory is one of several public semen analysis laboratories......Objective: Over recent decades a possible decrease in semen quality and an increase in the incidence of testicular cancer have been reported. In addition, men with poor semen quality have been reported to be at increased risk of developing testicular cancer whereas the risk of other cancers...... occurred first. Standardized mortality ratios (SMR) compared with total population of Danish men were calculated according to sperm concentration, motility, and morphology. Back to Top Results: Men with a sperm concentration between 1 and 9 and 10 and 19 million/mL had SMRs of 1.57 (95% CI 1.35–1.81) and 1...

  13. Lipid peroxidation in bovine semen.

    Science.gov (United States)

    Dawra, R K; Sharma, O P; Makkar, H P

    1983-01-01

    Bovine whole semen, spermatozoa, and seminal plasma did not undergo lipid peroxidation when aerobically incubated. However, lipid peroxidation was induced in washed spermatozoa in the presence of iron or iron plus sodium ascorbate, whereas heating, sonication, or treatment with proteolytic enzymes did not have any effect. The time required for formation of optimum concentration of lipid peroxides in washed spermatozoa is very short as compared to other systems. Lipid peroxides are entirely contributed by the lipid fraction of spermatozoa. Formation of lipid peroxides is completely inhibited by the presence of seminal plasma in incubation mixture.

  14. Seasonal variation in semen quality of Boer and Angora

    African Journals Online (AJOL)

    tage live sperm, determine the quality of the semen. The ob- ject of this trial was to evaluate the quality of Angora and. Boer goat semen collected throughout the year by means of artificial vagina and/or electrical stimulation, for the purpose of freezing semen and doing AI throughout the year. Procedure. Semen from five ...

  15. Aroma peculiarities of apricot (Armeniaca vulgaris Lam. and cherry-plum (Prunus cerasifera Ehrh. flowers

    Directory of Open Access Journals (Sweden)

    В. М. Горіна

    2013-02-01

    Full Text Available In the component composition of volatile solutions determining fragrance of the flowers in apricot and cherry-plum varieties and Prunus brigantiaca Vill. x Armeniaca vulgaris Lam. hybrids there are 36 highest hydrocarbons and benzaldehyde that prevail. There are fewer amounts of the solutions which scare bees (benzaldehyde in the fragrance of cherry-plum varieties as compared to the flowers of apricot and hybrids. At the same time, the content of tricosane, pentacosane, docosane, heneycosane, eicosane, nonadecan that probably attract bees is higher in the cherry-plum flowers than in the fragrance of apricot and hybrid flowers. The average three years yield of cherry-plum plants (Nikitska Zhovta 10,7 and Salgirskaya Rumjanaya 28,5 t/ ha is higher than for apricot (Recolte de Schatene 0,3; Rodnik 2,9; Ananasniy Tsurupinsky 7,4 t/ha and hybrids (8110 – 5,2; 8098 – 6,4 t/ha that could be explained with better pollination of flowers and better fruit formation. Prevailing components of flower aroma of these plants    and their possible link with yield of the objects in questions have been analyzed.

  16. Purification and biochemical characteristics of pectinesterase from Malatya apricot (Prunus armeniaca L.).

    Science.gov (United States)

    Ozler, Aynur; Karakuş, Emine; Pekyardimci, Sule

    2008-01-01

    Pectinesterase (PE) in Malatya apricot pulp (Prunus armeniaca L.) was extracted and purified through (NH(4))(2)SO(4) precipitation, dialysis, and DEAE-Sephadex gel filtration chromatography. The samples obtained from the dialysis procedure, named partially purified enzyme, were used for characterization of the apricot pectinesterase. The effect of various factors such as pH, temperature, heat, and storage stability on the partially purified apricot PE enzyme was investigated. Optimum pH value was 9.0 for PE with 1% pectin in 0.1 N NaCl (w/v). The optimum temperature for apricot PE was found to be 60 degrees C on standard analysis conditions. Heat inactivation studies showed a decrease in enzymatic activity at temperatures above 70 degrees C. Km and V(max) values were 0.77 mM and 1.75 micromol min(-1) mg(-1) for apricot PE. Five inhibitors were tested in the study; the most effective inhibitor was found to be sodium carbonate (100% inhibition). The order of inhibitory effectiveness was: Na(2)CO(3), iodine, lauril sulphate, AgNO(3), EDTA. Thermal inactivation data indicated that apparent activation energy with pectin substrate was 2.96 kcal mol(-1) for the enzyme. Ascorbic acid, CaCl(2), and KCl showed activatory effect on the apricot PE enzyme.

  17. Medicinal plants with hepatoprotective activity in Iranian folk medicine

    Directory of Open Access Journals (Sweden)

    Majid Asadi-Samani

    2015-02-01

    Full Text Available There are a number of medicinal combinations in the Iranian traditional medicine which are commonly used as tonic for liver. In this review, we have introduced some medicinal plants that are used mainly for the treatment of liver disorders in Iranian folk medicine, with focus on their hepatoprotective effects particularly against CC14 agent. In this study, online databases including Web of Science, PubMed, Scopus, and Science Direct were searched for papers published from January 1970 to December 2013. Search terms consisted of medicinal plants, traditional medicine, folk medicine, hepatoprotective, Iran, liver, therapeutic uses, compounds, antioxidant, CC14, anti-inflammatory, and antihepatotoxic, hepatitis, alone or in combination. Allium hirtifolium Boiss., Apium graveolens L., Cynara scolymus, Berberis vulgaris L., Calendula officinalis, Nigella sativa L., Taraxacum officinale, Tragopogon porrifolius, Prangos ferulacea L., Allium sativum, Marrubium vulgare, Ammi majus L., Citrullus lanatus Thunb, Agrimonia eupatoria L. and Prunus armeniaca L. are some of the medicinal plants that have been used for the treatment of liver disorders in Iranian folk medicine. Out of several leads obtained from plants containing potential hepatoprotective agents, silymarin, β-sitosterol, betalain, neoandrographolide, phyllanthin, andrographolide, curcumin, picroside, hypophyllanthin, kutkoside, and glycyrrhizin have been demonstrated to have potent hepatoprotective properties. Despite encouraging data on possibility of new discoveries in the near future, the evidence on treating viral hepatitis or other chronic liver diseases by herbal medications is not adequate.

  18. Degradation of cyanogenic glycosides of bitter apricot seeds (Prunus armeniaca) by endogenous and added enzymes as affected by heat treatments and particle size.

    NARCIS (Netherlands)

    Tuncel, G.; Nout, M.J.R.; Brimer, L.

    1998-01-01

    Bitter apricot (Prunus armeniaca) seeds (kernels) are by-products of the apricot processing industry. They contain approximately 50-150 μMol/g (dry weight basis) of potentially toxic cyanogenic glycosides, mainly amygdalin and prunasin. The present paper deals with the degradation of these

  19. Role of semen analysis in subfertile couples

    NARCIS (Netherlands)

    van der Steeg, Jan W.; Steures, Pieternel; Eijkemans, Marinus J. C.; F Habbema, J. Dik; Hompes, Peter G. A.; Kremer, Jan A. M.; van der Leeuw-Harmsen, Loes; Bossuyt, Patrick M. M.; Repping, Sjoerd; Silber, Sherman J.; Mol, Ben W. J.; van der Veen, Fulco

    2011-01-01

    Objective: To evaluate the associations between the results of the male partner's semen analysis (classified according to the World Health Organization [WHO] criteria) and fathering a child without any treatment. Design: Prospective multicenter cohort study. Setting: Twenty subfertility centers in

  20. Semen quality improves marginally during young adulthood

    DEFF Research Database (Denmark)

    Perheentupa, Antti; Sadov, Sergey; Rönkä, Riitta

    2016-01-01

    STUDY QUESTION: Does semen quality improve during early adulthood? SUMMARY ANSWER: Semen variables change little during the third decade of life, however some improvement in sperm morphology and motility may occur. WHAT IS KNOWN ALREADY: A suspicion of deteriorating semen quality has been raised...... in several studies. The longitudinal development of semen quality in early adulthood is insufficiently understood. STUDY DESIGN, SIZE, DURATION: A longitudinal follow-up of two cohorts of volunteer young adult Finnish men representing the general population was carried out. Cohorts A (discovery cohort, born...... participated both in the first round and in the final fourth round (cohort A, n = 111 and cohort B, n = 90 men) and in all four rounds (cohort A, n = 61 and cohort B, n = 52). WIDER IMPLICATIONS OF THE FINDINGS: Almost full spermatogenic capacity is reached by the age of 19 years. However, the improvement...

  1. Artificial insemination of cranes with frozen semen

    Science.gov (United States)

    Gee, G.F.; Sexton, T.J.; Lewis, J.C.

    1979-01-01

    For the first time (1978) artificial insemination (AI) with frozen greater sandhill crane (Grus canadensis tabida) semen resulted in fertile eggs and chicks. During the 2 year (1977-78) study, 6 of 27 eggs produced were fertile. Three chicks hatched. Semen samples used for insemination were frozen and stored in liquid nitrogen for two months or less. Recent improvements in the laboratory indicated that a more effective sample can be prepared and greater fertility rates should be expected.

  2. Ambient air pollution and semen quality.

    Science.gov (United States)

    Nobles, Carrie J; Schisterman, Enrique F; Ha, Sandie; Kim, Keewan; Mumford, Sunni L; Buck Louis, Germaine M; Chen, Zhen; Liu, Danping; Sherman, Seth; Mendola, Pauline

    2018-05-01

    Ambient air pollution is associated with systemic increases in oxidative stress, to which sperm are particularly sensitive. Although decrements in semen quality represent a key mechanism for impaired fecundability, prior research has not established a clear association between air pollution and semen quality. To address this, we evaluated the association between ambient air pollution and semen quality among men with moderate air pollution exposure. Of 501 couples in the LIFE study, 467 male partners provided one or more semen samples. Average residential exposure to criteria air pollutants and fine particle constituents in the 72 days before ejaculation was estimated using modified Community Multiscale Air Quality models. Generalized estimating equation models estimated the association between air pollutants and semen quality parameters (volume, count, percent hypo-osmotic swollen, motility, sperm head, morphology and sperm chromatin parameters). Models adjusted for age, body mass index, smoking and season. Most associations between air pollutants and semen parameters were small. However, associations were observed for an interquartile increase in fine particulates ≤2.5 µm and decreased sperm head size, including -0.22 (95% CI -0.34, -0.11) µm 2 for area, -0.06 (95% CI -0.09, -0.03) µm for length and -0.09 (95% CI -0.19, -0.06) µm for perimeter. Fine particulates were also associated with 1.03 (95% CI 0.40, 1.66) greater percent sperm head with acrosome. Air pollution exposure was not associated with semen quality, except for sperm head parameters. Moderate levels of ambient air pollution may not be a major contributor to semen quality. Published by Elsevier Inc.

  3. Cryopreservation of South African indigenous goat semen ...

    African Journals Online (AJOL)

    Semen was thereafter cryopreserved in straws using liquid nitrogen. Data were analysed using Stata® V10 software. South African indigenous goats had total sperm cell motility rate of 83.1%, progressive sperm cell motility of 49.3% and non-progressive sperm cell motility of 33.9%. Moreover, acidic semen pH of 6.4 and ...

  4. Human semen assays for workplace monitoring

    International Nuclear Information System (INIS)

    Wyrobek, A.J.; Gledhill, B.L.

    1978-01-01

    Decades of human semen studies have yielded compelling evidence that sperm can be used to access reproductive potential and diagnose pathology. With these studies as background, the small number of detailed semen studies of men exposed to physical and chemical agents point with optimism to the application of human semen assays as efficient, effective means to monitor for reproductive hazards in the workplace. Sperm are the most accessible of human gonadal tissue and provide a means of monitoring exposure induced changes in the human testes, changes which may result in infertility and increased frequencies of genetically abnormal gametes. The focus on semen has precipitated the development of new sperm bioassays which use older conventional andrological methods (i.e., sperm counts, motility, and morphology) as well as recently developed high speed flow and scanning methods for automated cytological analyses. The status of these sperm assays for workplace surveillance is reviewed, procedures are suggested with examples of use, and their effectiveness is evaluated. The available mouse models of induced semen changes are briefly described and the importance of these models for evaluating the genetic implications of findings in human semen is discussed

  5. Angka Konsepsi Hasil Inseminasi Semen Cair Versus Semen Beku pada Kuda yang Disinkronisasi Estrus dan Ovulasi

    Directory of Open Access Journals (Sweden)

    R. I. Arifiantini

    2010-04-01

    Full Text Available Semen used for artificial insemination (AI can be prepared in different ways, fresh extended fresh or chilled, or frozen-thawed (FT. The aim of the experiment was to evaluate the use of preserved semen to inseminate the oestrus mares. Nineteen mares were used in this research. The mares were synchronized with double injection of PGF2α 14 days apart. The follicle size was monitored using ultrasound scanner during the third day of oestrus, and 2500 IU hCG was administered at the same time. The AI was conducted 35 hours after hCG injection with total motile sperm 200x106 for chilled semen and 250-300x106 for frozen semen. The result demonstrated that the response of the oestrus with double injection of PGF2α was 73.7%. The conception rate (CR was 14.3% (1/7 with frozen semen and 42.9% (3/7 with chilled semen. It is concluded that AI with chilled semen resulted higher conception rate than frozen semen.

  6. Semen collection using phantom in dromedary camel.

    Science.gov (United States)

    Ziapour, S; Niasari-Naslaji, A; Mirtavousi, M; Keshavarz, M; Kalantari, A; Adel, H

    2014-12-10

    Semen collection is relatively long, unsafe, and tedious procedure in dromedary camel. The innovation of safe, hygienic, and simple approach to collect semen could make great progress in development of AI program in this species. This study investigated two methods of semen collection using phantom and artificial vagina in dromedary camel. Semen was collected using phantom (n = 4 bulls; 26 collections) and artificial vagina (n = 6 bulls; 11 collections) and diluted with INRA96 at the ratio of 1:10. The duration of semen collection, semen parameters, and morphometric features of sperm were evaluated. For specimen collected through phantom and AV, the respected duration of semen collection (411.2 ± 48.19 vs 326 ± 37.05 sec), volume (6.6 ± 0.87 vs 6 ± 1.57 ml), osmolarity (328 ± 1.6 vs 319.4 ± 3.21 mOsm/kg H2O), pH (7.7 ± 0.06 vs 7.9 ± 0.16) of semen, concentration (161.4 ± 44.05 × 10(6)/mL vs 160.2 ± 58.42 × 10(6)/mL), total motility (84.1 ± 1.89 vs 78.3 ± 3.97%), progressive forward motility (45.5 ± 3.69 vs 44.3 ± 6.41%), live percentage (72.2 ± 3.11 vs 76 ± 2.53%), and plasma membrane integrity (61.5 ± 2.49 vs 58.9 ± 4.19%) of sperm were similar (P > 0.05). The number of specimens contaminated with visible particles was greater using AV (72.7%) compared to phantom (0%; P < 0.05). Total length, head, middle-piece, and tail length of sperm were 45.9 ± 0.1, 5.6 ± 0.01, 7 ± 0.02, and 34.2 ± 0.16 μm, respectively. The frequency of abnormal sperm was 13.28% among which coiled tail, detached head, and proximal protoplasmic droplets had greater incidence. In conclusion, phantom could be considered as a suitable approach to collect semen due to simplicity, safety, and lack of specimen contamination in dromedary camel. Copyright © 2014 Elsevier B.V. All rights reserved.

  7. Vitamin B12 and Semen Quality

    Directory of Open Access Journals (Sweden)

    Saleem Ali Banihani

    2017-06-01

    Full Text Available Various studies have revealed the effects of vitamin B12, also named cobalamin, on semen quality and sperm physiology; however, these studies collectively are still unsummarized. Here, we systematically discuss and summarize the currently understood role of vitamin B12 on semen quality and sperm physiology. We searched the Web of Science, PubMed, and Scopus databases for only English language articles or abstracts from September 1961 to March 2017 (inclusive using the key words “vitamin B12” and “cobalamin” versus “sperm”. Certain relevant references were included to support the empirical as well as the mechanistic discussions. In conclusion, the mainstream published work demonstrates the positive effects of vitamin B12 on semen quality: first, by increasing sperm count, and by enhancing sperm motility and reducing sperm DNA damage, though there are a few in vivo system studies that have deliberated some adverse effects. The beneficial effects of vitamin B12 on semen quality may be due to increased functionality of reproductive organs, decreased homocysteine toxicity, reduced amounts of generated nitric oxide, decreased levels of oxidative damage to sperm, reduced amount of energy produced by spermatozoa, decreased inflammation-induced semen impairment, and control of nuclear factor-κB activation. However, additional research, mainly clinical, is still needed to confirm these positive effects.

  8. Kualitas semen dan produksi semen beku pada bangsa sapi dan bulan penampungan yang berbeda

    Directory of Open Access Journals (Sweden)

    Mita Ayu Rahmawati

    2015-12-01

    Full Text Available The purpose of this research was to determine semen quality and frozen semen production of beef cattle at different month collection in Lembang Artificial Insemination Center. Beef cattle strain that used for this research are Aceh, Angus, Brahman, Limousine, Madura, Ongole and Simmental. The research parameter included semen volume, pH, motility of spermatozoa, concentration of spermatozoa, number of spermatozoa, number of spermatozoa motil and number of frozen semen straw. The method of this research using factorial completely Randomized Design and each had 3 replication per treatment. The different of beef cattle have given a significant effect of semen volume (P0.05. Different month collection have a significant influence of semen volume (P<0.01, pH (P<0.05, motility of spermatozoa (P<0.01, concentration of spermatozoa (P<0.01, number of spermatozoa (P<0.01, number of spermatozoa motil (P<0.01. Interaction between breed of beef cattle and different month of collection have given a significant influence of semen volume (P<0.01, motility spermatozoa (P<0.01, concentration spermatozoa (P<0.05 and number of spermatozoa motil (P<0.01. Keywords: sperm motility, concentration, number of motile spermatozoa

  9. Spermatological Parameters of Extended Rabbit Semen in 5 ...

    African Journals Online (AJOL)

    USER

    validation of semen samples to be used in artificial insemination. This study was aimed at evaluating the effect of 5% egg yolk plasma from 5 Nigerian domesticated avian species as a component of semen extender in the preservation of rabbit semen to be used for field fertility trials. The evaluated parameters (%) pre and ...

  10. Quantitative aspects of semen imports for South African dairy breeds

    African Journals Online (AJOL)

    Dairy cattle semen imported from January 1979 to June 1989 consisted of 72% Holstein-Friesian, 13.6% Jersey,. 11.5% Ayrshire and 2.8% ... South African importers by importing semen of mainly two lines could possibly narrow the genetic base of the South ..... qualified for semen export to South Africa during 1990. Most.

  11. Seasonal changes in sexual activity and semen quality in the ...

    African Journals Online (AJOL)

    Seasonal changes in semen volume and quality in the Angora ram were investigated. Semen was collected by means of an artificial vagina and criteria such as ejaculate volume, semen pH, sperm concentration, sperm motility, percentage live and normal sperm and survival of sperm after freezing and thawing were ...

  12. Revisit Of Current Patterns Of Semen Quality Among Male Partners ...

    African Journals Online (AJOL)

    About 61% of the patients produced semen by coitus interruptus while, 34.3% obtained their samples by masturbation. Majority of the patients (70.1%) produced semen volume of greater than 2mls, while 29.9% produced semen of less than 2mls. Of the total number, 34.7% were normozoospermia while, 65.3% had one ...

  13. Effects of ground semen collection on weight bearing on hindquarters, libido, and semen parameters in stallions.

    Science.gov (United States)

    Burger, D; Meroni, G; Thomas, S; Sieme, H

    2015-09-15

    Collection of semen on the ground from the standing stallion represents an alternative method to dummy mount semen collection and is of increasing popularity for sport stallions, males suffering from health problems, or in studs without a dummy or suitable mare at disposal. Our aim was to collect and compare spermatological and physiological data associated with traditional and ground semen collection. Twelve of 23 Franches-Montagnes stallions were selected to carry out semen collection on a dummy and while standing in a crossed experimental protocol. Semen quantity and quality parameters, weight bearing on hindquarters, and behavioral and libido data were recorded. Ground versus dummy mount semen collection was accompanied by lower seminal volume (15.9 ± 14.6 vs. 22.0 ± 13.3 mL; P < 0.01) and lower total sperm count (4.913 ± 2.721 × 10(9) vs. 6.544 ± 2.856 × 10(9) sperm; P < 0.001). No significant differences were found concerning sperm motility and viability. Time to ejaculation was longer, and the number of attempts to ejaculation was higher (P = 0.053) in the standing position compared with the mount on the dummy. A higher (P < 0.01) amount of tail flagging was manifested by the stallions during ejaculation on the dummy compared to when standing. There was no difference in weight bearing on hindquarters when comparing dummy collection (51.2 ± 2.5%) and standing collection (48.9 ± 5.5%). Ground semen collection can be considered as a viable option for stallions that cannot mount a dummy or a mare. However, it requires training and may be not easily accepted by all stallions. Owners should be advised that ground semen collection is associated with significantly lower sperm numbers than with dummy mount semen collection. Copyright © 2015 Elsevier Inc. All rights reserved.

  14. Semen quality in Peruvian pesticide applicators: association between urinary organophosphate metabolites and semen parameters

    Directory of Open Access Journals (Sweden)

    Gasco Manuel

    2008-11-01

    Full Text Available Abstract Background Organophosphates are broad class of chemicals widely used as pesticides throughout the world. We performed a cross-sectional study of associations between dialkylphosphate metabolites of organophosphates and semen quality among pesticide applicators in Majes (Arequipa, Peru. Methods Thirty-one men exposed to organophosphate (OP pesticides and 31 non-exposed were recruited (age, 20–60 years. In exposed subjects, semen and a blood sample were obtained one day after the last pesticide application. Subjects were grouped according to levels of OP metabolites in urine. Semen samples were analyzed for sperm concentration, percentage of sperm motility, percentage of normal morphology, semen leucocytes and concentrations of fructose and zinc. Exposure to OP was assessed by measuring six urinary OP metabolites (dimethyl and diethyl phosphates and thiophosphates by gas chromatography using a single flame photometric detector. Results Diethyldithiophosphate (p = 0.04 and diethylthiophosphate (p = 0.02 better reflected occupational pesticide exposure than other OP metabolites. Semen analysis revealed a significant reduction of semen volume and an increase in semen pH in men with OP metabolites. Multiple regression analysis showed that both occupational exposure to pesticides and the time of exposure to pesticides were more closely related to alterations in semen quality parameters than the single measurement of OP metabolites in urine. Conclusion The study demonstrated that occupational exposure to OP pesticides was more closely related to alterations in semen quality than a single measurement of urine OP metabolites. Current measurement of OP metabolites in urine may not reflect the full risk.

  15. The effect of Coicis semen and Rhizopus oligosporus-fermented Coicis semen (tempeh) on serum cholesterol in the rat

    OpenAIRE

    岡本, 基; 臼井, 真一; 岡崎, 三代

    2004-01-01

    The effect of Coicis semen and Rhizopus oligosporus-fermented Coicis semen (tempeh) on serum cholesterol fractions was examined in the rat. Twelve-week-old male Sprague-Dawley rats were received commercial MF meal (control group), Coicis semen-containing MF meal (Coicis semen group), or tempeh-containing MF meal (tempeh group). Cholesterol fractions were analyzed by high performance liquid chromatography at the start of experiment, and 2, 6, 18 weeks later. No difference was found in body wei...

  16. Effect of captopril on semen quality.

    Science.gov (United States)

    Banihani, S A

    2017-05-01

    Various studies (direct and indirect) have presented the effect of captopril, a universally used antihypertensive medication, on semen quality; yet, this effect is still collectively unreviewed. This review systematically discusses and summarises the effect of captopril on semen quality. We searched all published articles in the MEDLINE electronic database since June 1985 until January 2016 using the keywords "captopril" and "sperm," and certain supporting articles were reviewed and considered, if relevant. In conclusion, up to the present time, captopril does not appear to induce a striking change in semen quality, and hence on male infertility, while it may affect the rate of spermatozoa-egg fusion as it inhibits the activity of angiotensin-converting enzyme that is released during capacitation and the acrosome reaction. Further research, mainly clinical, is still desired to prove these effects. © 2016 Blackwell Verlag GmbH.

  17. Urethral anatomy and semen flow during ejaculation

    Science.gov (United States)

    Kelly, Diane

    2016-11-01

    Ejaculation is critical for reproductive success in many animals, but little is known about its hydrodynamics. In mammals, ejaculation pushes semen along the length of the penis through the urethra. Although the urethra also carries urine during micturition, the flow dynamics of micturition and ejaculation differ: semen is more viscous than urine, and the pressure that drives its flow is derived primarily from the rhythmic contractions of muscles at the base of the penis, which produce pulsatile rather than steady flow. In contrast, Johnston et al. (2014) describe a steady flow of semen through the crocodilian urethral groove during ejaculation. Anatomical differences of tissues associated with mammalian and crocodilian urethral structures may underlie these differences in flow behavior.

  18. Effect of alternate day collection on semen quality of Asian elephants (Elephas maximus) with poor initial fresh semen quality

    NARCIS (Netherlands)

    Imrat, P.; Mahasawangkul, Sittidet; Thitaram, Chatchote; Suthanmapinanth, P.; Kornkaewrat, K.; Sombutputorn, P.; Jansittiwate, S.; Thongtip, Nikorn; Pinyopummin, A.; Colenbrander, B.; Holt, W.V.; Stout, Tom

    2014-01-01

    In captivity, male Asian elephants often yield poor quality semen after transrectal manually assisted semen collection; however, the reasons for the disappointing semen quality are not clear. Here we test the hypothesis that accumulation of senescent spermatozoa is a contributory factor, and that

  19. 9 CFR 98.34 - Import permits for poultry semen and animal semen.

    Science.gov (United States)

    2010-01-01

    ... of entry for presentation to the collector of customs. Animal semen intended for importation into the...). Raw animal food wastes (garbage) shall not be fed to the donor boars while in isolation. At the start...

  20. Collection and characterization of semen from green iguanas (Iguana iguana).

    Science.gov (United States)

    Zimmerman, Dawn M; Mitchell, Mark A; Perry, Brittany Heggem

    2013-12-01

    To determine an efficient method for the collection of semen samples by means of electroejaculation, characterize spermatozoa quality and quantity, and determine the effect of refrigerated storage on motility of spermatozoa obtained from green iguanas (Iguana iguana). 18 adult green iguanas. Green iguanas were anesthetized, and semen samples were obtained by means of electroejaculation. Up to 3 series of electrostimulations were performed; the procedure was stopped after a semen sample was obtained. Various semen sample variables were evaluated. Semen samples were obtained from 16 iguanas; most (n = 10) iguanas produced a semen sample after the second series of electrostimulations. Median semen sample volume was 0.05 mL. Mean spermatozoa concentration was 2 69.0 × 10(6) spermatozoa/mL. Median percentage of motile spermatozoa was 78%. The only morphological abnormality of spermatozoa was bent tails (mean percentage in a semen sample, 5.7%). Spermatozoa motility decreased significantly during refrigeration (4°C); median percentage motility after 24, 48, and 72 hours of refrigeration was 60%, 33%, and 0%, respectively. Results of this study suggested electroejaculation can be performed to collect semen samples from green iguanas, characteristics of iguana semen samples are similar to those for semen samples obtained from other reptiles, and motility of iguana spermatozoa decreases during refrigeration within 48 to 72 hours.

  1. Semen quality in the 21(st) century

    DEFF Research Database (Denmark)

    Virtanen, Helena E; Jørgensen, Niels; Toppari, Jorma

    2017-01-01

    Although semen quality is an important determinant of fertility, defining clear thresholds for normal ranges has proven difficult. According to 'time to pregnancy' studies, fecundity starts to decline when sperm concentrations fall below 30-55 × 10(6)/ml, whereas the WHO criterion for normal values...

  2. Cryopreservation of South African indigenous goat semen

    African Journals Online (AJOL)

    use

    2011-12-05

    Dec 5, 2011 ... motility rates of South African indigenous goats. Reduction in the sperm cell motility after freeze/thawing is still a problem and requires further research on the diluents and techniques that give protection to sperm cells during cryopreservation. Key words: Cryopreservation, semen characteristics, indigenous ...

  3. [Quality standard study on Entadae Semen].

    Science.gov (United States)

    Shen, Hui-ping; Wang, Meng-yue; Li, Xiao-bo

    2015-05-01

    To establish the quality standard of Entadae Semen, and provide scientific basis for its quality control. TLC and HPLC were used for qualitative identification and quantitative analysis of phaseoloidin and entadamide A-O-β-D-glucopyranoside in Entadae Semen. The test of water content, ash and ethanol-soluble extractives of Entadae Semen was carried out according to the methods recorded in appendix of Chinese Pharmacopeia (2010 edition). The TLC was well separated with clear spots. The linear range of phaseoloidin was between 0.014 to 2.747 g x L(-1) (r = 0.999 6, n = 9) with an average recovery rate of 101.06% (RSD 0.90%, n = 6); the linear range of entadamide A-O-β-D-glucopyranoside was between 0.002 to 0.452 g x L(-1) (r = 0.999 7, n = 9) withan average recovery rate of 101.52% (RSD 1.09%, n = 6). The content of phaseoloidin in sample is between 5.12% to 9.24%, entadamide A-O-β-D-glucopyranoside is between 0.55% to 2.17%, alcohol-soluble extracts is between 30.9% to 45.2%, water is between 6.6% to 8.6%, and total ash is between 2.4% to 2.9%. The established standard is acceptable for quality control of Entadae Semen.

  4. Impact of cryopreservation on bull () semen proteome.

    Science.gov (United States)

    Westfalewicz, B; Dietrich, M A; Ciereszko, A

    2015-11-01

    Cryopreservation of bull spermatozoa is a well-established technique, allowing artificial insemination of cattle on a commercial scale. However, the extent of proteome changes in seminal plasma and spermatozoa during cryopreservation are not yet fully known. The objective of this study was to compare the proteomes of fresh, equilibrated, and cryopreserved bull semen (spermatozoa and seminal plasma) to establish the changes in semen proteins during the cryopreservation process. Semen was collected from 6 mature Holstein Friesian bulls. After sample processing, comparative analysis and identification of proteins was performed using 2-dimensional difference in-gel electrophoresis coupled with matrix-assisted laser desorption/ionization mass spectrometry. Analysis of spermatozoa extracts revealed that 25 identified protein spots, representing 16 proteins, underwent significant ( cryopreservation. Eighteen protein spots decreased in abundance, 5 protein spots increased in abundance, and 2 protein spots showed different, specific patterns of abundance changes. Analysis of seminal fluid containing seminal plasma showed that 6 identified protein spots, representing 4 proteins, underwent significant ( cryopreservation. Two protein spots increased in abundance and 4 decreased in abundance. Semen extending and equilibration seems to be responsible for a significant portion of the proteome changes related to cryopreservation technology. Most sperm proteins affected by equilibration and cryopreservation are membrane bound, and loss of those proteins may reduce natural spermatozoa coating. Further research is needed to unravel the mechanisms of the particular protein changes described in this study and establish the relationship between those changes and sperm quality.

  5. Correlation of phthalate exposures with semen quality

    International Nuclear Information System (INIS)

    Pant, Niraj; Shukla, Manju; Kumar Patel, Devendra; Shukla, Yogeshwar; Mathur, Neeraj; Kumar Gupta, Yogendra; Saxena, Daya Krishna

    2008-01-01

    Phthalates are widely used man-made chemical released in the environment and human exposure is mainly through diet. As the phthalate plasticizers are not covalently bound to PVC, they can leach, migrate or evaporate into the environment and as a result have become ubiquitously contaminants. The present study investigates the correlation, if any, between the phthalate esters (DEP, DEHP, DBP, DMP, DOP) and sperm mitochondrial status, ROS, LPO, SCSA, and sperm quality. The study was conducted in the urban/rural population of Lucknow visiting Obstetrics and Gynecology Department, CSMMU, Lucknow. Semen analysis was performed according to the WHO guidelines while phthalate analysis by HPLC and LPO by spectrophotometer and the sperm mitochondrial status, ROS, SCSA using flow cytometry. The questionnaire data showed no significant difference in the demographic characteristics among the groups. In general, urban population was found to have statistically significant higher levels of phthalate esters than the rural. Further, infertile men showed statistically significant (p < 0.05) higher levels of pollutants in the semen than fertile men. A negative correlation between semen phthalate level viz DEHP and sperm quality and positive association with depolarized mitochondria, elevation in ROS production and LPO, DNA fragmentation was established. The findings are suggestive that phthalates might be one among the contributing factors associated with the deterioration in semen quality and these adverse effects might be ROS, LPO and mitochondrial dysfunction mediated

  6. Chemical composition and general toxicity of essential oils extracted from the aerial parts of Artemisia armeniaca Lam. and A. incana (L.) Druce growing in Iran.

    Science.gov (United States)

    Mojarrab, M; Delazar, A; Esnaashari, S; Afshar, F Heshmati

    2013-01-01

    The essential oils of the aerial parts of A. armeniaca and A. incana, collected from Arasbaran area (East Azarbaijan province, Iran) were extracted by hydrodistillation and analyzed by GC-MS. In total, 16 and 40 constituents were identified and quantified in the oils of A. armeniaca and A. incana representing 80.5% and 84.6% of the oils, respectively. The essential oil of A. armeniaca was mainly composed of non-terpene hydrocarbons (24.8%). The major components of the oil were α-pinene (10.7%), nonadecane (10.0%), 6,10,14-trimethyl-2-pentadecanone (9.4%), spathulenol (7.8%) and Z-verbenol (5.8%). The essential oil of A. incana was dominated by oxygenated monoterpenes (41.6%), with camphor (20.4%), 1,8-cineol (10.3%), Z-verbenol (8.7%), β-thujone (8.3%) and α-thujone (5.6%), as major components. The essential oils were also subjected to general toxicity assay using brine shrimp lethality method. The toxicity profile of both oils indicated some degree of toxicity in comparison with podophyllotoxin.

  7. Cryopreservation of Sambar deer semen in Thailand.

    Science.gov (United States)

    Vongpralub, Thevin; Chinchiyanond, Wittaya; Hongkuntod, Pornchai; Sanchaisuriya, Pitcharat; Liangpaiboon, Sanan; Thongprayoon, Areeya; Somphol, Noppadon

    2015-01-01

    Little is known of the different freezing and thawing techniques for post-thaw survival of spermatozoa in Sambar deer. So, this study determined the effect of seminal plasma, egg yolk and glycerol extenders and their concentrations, plus cooling, freezing, and thawing protocols on the post-thaw quality of their semen. Semen samples were collected by electro-ejaculation from four Thai Sambar deer stags (Cervus unicolor equinus). As evaluated by post-thaw progressive motility and acrosome integrity removal of seminal plasma was beneficial; Tris-egg yolk was the most efficient extender; a 20% egg yolk concentration was better than the 0%, 10%, or 30%; and a 3% glycerol concentration was better than 5%, 7%, or 9%. Using the optimum dilution techniques, semen was loaded in 0.5 ml plastic straws. Cooling times from ambient temperature to 5°C in 3 hr resulted in higher post-thaw progressive motility and acrosome integrity than 1, 2, or 4 hr. Suspending the straws 4 cm above the surface for 15 min before plunging into liquid nitrogen was better than suspending at 2 or 6 cm. For thawing frozen semen, an intermediate thawing (50°C, 8 sec) protocol was more effective than the slower (37°C, 10 sec) or faster (70°C, 5 sec) thawing rates. Timed insemination following estrus synchronization of 10 hinds resulted in six confirmed pregnancies at 60 days. Five hinds delivered live fawns. This study provides an effective approach for semen cryopreservation and artificial insemination (AI), which should be valuable to scientists for genetics and reproductive management of Sambar deer in developing countries. © 2015 Wiley Periodicals, Inc.

  8. Prevalence of mycoplasmas in the semen and vaginal swabs of Danish stallions and mares

    DEFF Research Database (Denmark)

    Baczynska, Agata; Fedder, Jens; Schougaard, Hans

    2007-01-01

    The reproduction rate of horses is one of the lowest within domestic livestock despite advances the veterinary medicine. Infertility in horses may be due mainly to the lack of suitable selection criteria in the breeding of horses. However, acquired infertility due to genital, bacterial infections...... may occur. Mycoplasmas have been implicated in genital disorders and infertility of many species including humans and horses. However, their role as commensals or pathogens of the genital tract of horses is still not determined. Bacteriological examinations made on the fossa glandis, urethra, penis...... and semen of stallions, showed the presence of different Mycoplasma species. Therefore our study aimed to find the prevalence of Mycoplasma species and a possible association with fertility problems in Danish riding horses. Eighty semen samples from stallions and 19 vaginal swab samples from mares were...

  9. Effect of Age and Bulls on Fresh Semen Quality and Frozen Semen Production of Holstein Bulls in Indonesia

    Science.gov (United States)

    Argiris, A.; Ondho, Y. S.; Santoso, S. I.; Kurnianto, E.

    2018-02-01

    Artificial Insemination is a compatible method of reproduction in an effort to increase dairy productivity. Artificial Insemination Center as a producer of frozen semen was required to maximize bulls in producing high quality frozen semen optimally. The purpose of this research was to determine effect of age and bulls on fresh semen quality and frozen semen production of Holstein bulls in Indonesia. The research was conducted at Lembang and Singosari AI Centers. The material used were 24.634 data of qualified fresh semen and frozen semen production from 81 Holstein Bulls aged 1-9 years that used as frozen semen producer in period of 2008 to 2016. The variables observed in this research were data of age of bulls, fresh semen volume (mL); sperm motility (%); mass movement; concentrations (million/mL) and frozen semen doses at each production at age of bulls. Nested design was applied to obtain and analyze data. Results showed that Age and bulls have significant effect (PAI Centre might adapt management of AI bulls to improve semen production.

  10. Semen phthalate metabolites, semen quality parameters and serum reproductive hormones: A cross-sectional study in China.

    Science.gov (United States)

    Wang, Yi-Xin; Zeng, Qiang; Sun, Yang; Yang, Pan; Wang, Peng; Li, Jin; Huang, Zhen; You, Ling; Huang, Yue-Hui; Wang, Cheng; Li, Yu-Feng; Lu, Wen-Qing

    2016-04-01

    Exposure to phthalates has been found to have adverse effects on male reproductive function in animals. However, the findings from human studies are inconsistent. Here we examined the associations of phthalate exposure with semen quality and reproductive hormones in a Chinese population using phthalate metabolite concentrations measured in semen as biomarkers. Semen (n = 687) and blood samples (n = 342) were collected from the male partners of sub-fertile couples who presented to the Reproductive Center of Tongji Hospital in Wuhan, China. Semen quality parameters and serum reproductive hormone levels were determined. Semen concentrations of 8 phthalate metabolites were assessed using high-performance liquid chromatography and tandem mass spectrometry. Associations of the semen phthalate metabolites with semen quality parameters and serum reproductive hormones were assessed using confounder-adjusted linear and logistic regression models. Semen phthalate metabolites were significantly associated with decreases in semen volume [mono-n-butyl phthalate (MBP), mono-(2-ethylhexyl) phthalate (MEHP), mono(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP), mono(2-ethyl-5-oxohexyl) phthalate (MEOHP)], sperm curvilinear velocity [monobenzyl phthalate (MBzP), MEHP, the percentage of di-(2-ethylhexyl)-phthalate metabolites excreted as MEHP (%MEHP)], and straight-line velocity (MBzP, MEHP, %MEHP), and also associated with an increased percentage of abnormal heads and tails (MBzP) (all p for trend hormones. Our findings suggest that environmental exposure to phthalates may impair human semen quality. Copyright © 2015 Elsevier Ltd. All rights reserved.

  11. Chemical transitions of Areca semen during the thermal processing revealed by temperature-resolved ATR-FTIR spectroscopy and two-dimensional correlation analysis

    Science.gov (United States)

    Wang, Zhibiao; Wang, Xu; Pei, Wenxuan; Li, Sen; Sun, Suqin; Zhou, Qun; Chen, Jianbo

    2018-03-01

    Areca semen is a common herb used in traditional Chinese medicine, but alkaloids in this herb are categorized as Group I carcinogens by IARC. It has been proven that the stir-baking process can reduce alkaloids in Areca semen while keep the activity for promoting digestion. However, the changes of compositions other than alkaloids during the thermal processing are unclear. Understanding the thermal chemical transitions of Areca semen is necessary to explore the processing mechanisms and optimize the procedures. In this research, FTIR spectroscopy with a temperature-controlled ATR accessory is employed to study the heating process of Areca semen. Principal component analysis and two-dimensional correlation spectroscopy are used to interpret the spectra to reveal the chemical transitions of Areca semen in different temperature ranges. The loss of a few volatile compounds in the testa and sperm happens below 105 °C, while some esters in the sperm decreases above 105 °C. As the heating temperature is close to 210 °C, Areca semen begins to be scorched and the decomposition of many compounds can be observed. This research shows the potential of the temperature-resolved ATR-FTIR spectroscopy in exploring the chemical transitions of the thermal processing of herbal materials.

  12. Monitoring environmental exposures with semen assays

    International Nuclear Information System (INIS)

    Anon.

    1979-01-01

    Semen studies in humans and animals have yielded extensive and compelling evidence that sperm can be used to assess reproductive potential and diagnose pathology. More recent studies on mutagens and carcinogens both at this and other laboratories suggest that a combination of mouse and human assays can be an efficient, effective approach to monitoring for reproductive hazards in the environment. We are investigating the potential of using variability in sperm morphology and DNA content to quantify and monitor the effects of environmental agents on the human testes. Here we review the status of human and mouse assays for environmental surveillance, discuss the genetic and fertility implications of chemically induced semen changes, and describe the high-speed flow methods being developed to automate sperm assays

  13. Semen quality of Italian local pig breeds

    Directory of Open Access Journals (Sweden)

    G. Gandini

    2010-01-01

    Full Text Available From 1996 to 1999 a conservation programme was carried out within the framework of EC contract “European gene banking project for the pig genetic resources” (Ollivier et al., 2001 in the Italian local pig breeds. The aims of the program included the primary characterization of the breeds, i.e. information on the organization in charge of the breed, breeding population numbers, breed description and qualifications, and field trials on productive and reproductive performances. In this context the “Semen Bank of Italian local pig breeds” was built. A total of 30,835 straws of four Italian local pig breeds (Cinta Senese, Casertana, Mora Romagnola and Nero Siciliano, collected from 42 sires, have been stored. In this work semen quality traits, lipid composition and freezability of the four Italian local pig breeds are reported.

  14. Microbial quality of equine frozen semen.

    Science.gov (United States)

    Corona, A; Cherchi, R

    2009-10-01

    Bacteriological surveillance is little applied in management of equine frozen semen but it is quite important to verify the microbial contamination in order to find out the chance of transmission of pathology to the mare in AI. Authors describe a qualitative and quantitative analysis for bacterial contamination on long time (3-17 years) equine frozen semen stored in liquid nitrogen. The semen checked, produced in Italy and in another Europe country, was cryopreserved in liquid nitrogen inside sealed plastic straws. One hundred and ten straws were checked out for pathogenic and no pathogenic bacteria, aerobes and anaerobes and fungi (moulds and yeasts). The Total Microbial Charge was quite variable with an average of about 1.4 x 10(5)CFU/ml. Mostly the microbial agents identified were fungi (17.5%), Enterobacter-coccus spp. (15%), Pseudomonas spp. (6.25%), Stenothophomonas maltophila (6.25%) and anaerobic bacteria like Propionibacterium granulosum (7.5%) and Clostridium spp. (3.75%). 3.75% were unidentified Gram-negative rod and cocci. Streptococcus spp., Staph. aureus, E. coli, Th. equigenitalis and Mycoplasma spp. were not detected. The most represented species were Enterobacter-coccus spp. (1.1 x 10(5)CFU/ml), St. maltophila (8 x 10(4)CFU/ml) and Pr. granulosum (7 x 10(4)CFU/ml) while yeast and even more moulds were little abundant (4.7 x 10(4) and 3.4 x 10(4)CFU/ml respectively). The knowledge of equine frozen semen microbial quality is essential to check out transmission of venereal disease and improve the quality of cryopreserved germplasm.

  15. Sperm ubiquitination in epididymal feline semen.

    Science.gov (United States)

    Vernocchi, Valentina; Morselli, Maria Giorgia; Varesi, Sara; Nonnis, Simona; Maffioli, Elisa; Negri, Armando; Tedeschi, Gabriella; Luvoni, Gaia Cecilia

    2014-09-01

    Ubiquitin is a 8.5-kDa peptide that tags other proteins for proteasomal degradation. It has been proposed that ubiquitination might be responsible for the elimination of defective spermatozoa during transit through the epididymis in humans and cattle, but its exact biological function in seminal plasma has not yet been clarified. In the domestic cat (Felis catus), the percentage of immature, unviable, and abnormal spermatozoa decreases during the epididymal transit, indicating the existence of a mechanism that removes defective spermatozoa. Magnetic cell separation techniques, based on the use of magnetic beads coated with anti-ubiquitin antibodies, may allow the selective capture of ubiquitinated spermatozoa from semen, thus contributing to the identification of a potential correlation between semen quality and ubiquitination process. Moreover, the selective identification of all the ubiquitinated proteins in different epididymal regions could give a better understanding of the ubiquitin role in feline sperm maturation. The aims of this study were as follows: (1) to verify the possibility of separating ubiquitinated spermatozoa with magnetic ubiquitin beads and identify the morphological and acrosomal differences between whole sample and unbound gametes, (2) to characterize all the ubiquitinated proteins in spermatozoa retrieved in the three epididymal regions by a proteomic approach. The data indicated the presence of ubiquitinated proteins in cat epididymal semen. However, a correlation between abnormal and ubiquitinated spermatozoa has not been found, and ubiquitin cannot be considered as a biomarker of quality of epididymal feline spermatozoa. To the author's knowledge, this is the first identification of all the ubiquitinated proteins of cat spermatozoa collected from different epididymal regions. The proteomic pattern allows a further characterization of cat epididymal semen and represents a contribute to a better understanding of the ubiquitin role in

  16. ALKALINE PHOSPHATASE ACTIVITY AS A MARKER OF DOG SEMEN FREEZABILITY

    Directory of Open Access Journals (Sweden)

    KOSINIAK-KAMYSZ K.

    2007-01-01

    Full Text Available The investigation was performed to evaluate the dog semen freezability and itsquality after thawing allowing its use for artificial insemination (AI. On the basis ofsperm motility, concentration and alkaline phosphatase (AP activity in semenplasma it was possible to establish that AP activity corresponds with the basic factorof semen examination. Significant statistical differences occurred between thequality of ejaculates which were qualified or disqualified to deep freezing and AI.These results show that AP activity in raw dog semen plasma can be used as amarker for the dog semen qualification for deep freezing and AI with 95%probability of the prognosis of the results.

  17. ALKALINE PHOSPHATASE ACTIVITY AS A MARKER OF DOG SEMEN FREEZABILITY

    Directory of Open Access Journals (Sweden)

    K. KOSINIAK-KAMYSZ

    2007-05-01

    Full Text Available The investigation was performed to evaluate the dog semen freezability and itsquality after thawing allowing its use for artificial insemination (AI. On the basis ofsperm motility, concentration and alkaline phosphatase (AP activity in semenplasma it was possible to establish that AP activity corresponds with the basic factorof semen examination. Significant statistical differences occurred between thequality of ejaculates which were qualified or disqualified to deep freezing and AI.These results show that AP activity in raw dog semen plasma can be used as amarker for the dog semen qualification for deep freezing and AI with 95%probability of the prognosis of the results.

  18. Uranium quantification in semen by inductively coupled plasma mass spectrometry

    Science.gov (United States)

    Todorov, Todor; Ejnik, John W.; Guandalini, Gustavo S.; Xu, Hanna; Hoover, Dennis; Anderson, Larry W.; Squibb, Katherine; McDiarmid, Melissa A.; Centeno, Jose A.

    2013-01-01

    In this study we report uranium analysis for human semen samples. Uranium quantification was performed by inductively coupled plasma mass spectrometry. No additives, such as chymotrypsin or bovine serum albumin, were used for semen liquefaction, as they showed significant uranium content. For method validation we spiked 2 g aliquots of pooled control semen at three different levels of uranium: low at 5 pg/g, medium at 50 pg/g, and high at 1000 pg/g. The detection limit was determined to be 0.8 pg/g uranium in human semen. The data reproduced within 1.4–7% RSD and spike recoveries were 97–100%. The uranium level of the unspiked, pooled control semen was 2.9 pg/g of semen (n = 10). In addition six semen samples from a cohort of Veterans exposed to depleted uranium (DU) in the 1991 Gulf War were analyzed with no knowledge of their exposure history. Uranium levels in the Veterans’ semen samples ranged from undetectable (<0.8 pg/g) to 3350 pg/g. This wide concentration range for uranium in semen is consistent with known differences in current DU body burdens in these individuals, some of whom have retained embedded DU fragments.

  19. Assessment of motion and kinematic characteristics of frozen-thawed Sirohi goat semen using computer-assisted semen analysis.

    Science.gov (United States)

    Anand, Mukul; Yadav, Sarvajeet

    2016-02-01

    The aim was to determine the motion and kinematics characteristic of frozen-thawed spermatozoa in Sirohi goat using computer-assisted semen analysis. A study was carried out in Sirohi buck. Semen collection was made biweekly from each buck with the help of artificial vagina. A total of 12 ejaculates were collected from two bucks (six ejaculates from each buck). Freshly collected semen was pooled and later evaluated. The pooled semen sample was extended with standard glycerolated egg yolk tris extender and later subjected to a process of cryopreservation. The motion and kinematic characteristics of spermatozoa were studied during freez-thawing process. Significantly (plive percent, hypo-osmotic swelling test, and acrosomal integrity were recorded in neat semen followed by diluted and frozen thaw semen. The proportion of spermatozoa showing slow progression were the highest in the neat and diluted semen followed by rapid and non-progressively motile, while a reverse pattern was observed in the frozen thaw semen where the proportion of non-progressively motile spermatozoa were significantly (pvitality, plasma membrane integrity, and acrosome status were obtained in the neat semen followed by diluted and frozen thaw semen. Further, the process of cryopreservation results in a shift of motility from slow to non-progressive in the post-thaw semen with a significant decrease in the path velocities when compared to neat and diluted semen. Hence, it can be concluded that freezing-thawing process reduces the motility and kinematic characters spermatozoa and may be an important factor affecting the fertilizing ability of spermatozoa resulting in poor conception rate after insemination in goats.

  20. Assessment of motion and kinematic characteristics of frozen-thawed Sirohi goat semen using computer-assisted semen analysis

    Directory of Open Access Journals (Sweden)

    Mukul Anand

    2016-02-01

    Full Text Available Aim: The aim was to determine the motion and kinematics characteristic of frozen-thawed spermatozoa in Sirohi goat using computer-assisted semen analysis. Materials and Methods: A study was carried out in Sirohi buck. Semen collection was made biweekly from each buck with the help of artificial vagina. A total of 12 ejaculates were collected from two bucks (six ejaculates from each buck. Freshly collected semen was pooled and later evaluated. The pooled semen sample was extended with standard glycerolated egg yolk tris extender and later subjected to a process of cryopreservation. The motion and kinematic characteristics of spermatozoa were studied during freez-thawing process. Results: Significantly (p<0.01 higher value of live percent, hypo-osmotic swelling test, and acrosomal integrity were recorded in neat semen followed by diluted and frozen thaw semen. The proportion of spermatozoa showing slow progression were the highest in the neat and diluted semen followed by rapid and non-progressively motile, while a reverse pattern was observed in the frozen thaw semen where the proportion of non-progressively motile spermatozoa were significantly (p<0.01 higher followed by slow and rapid progression. Conclusion: This study showed that the best results for motion, vitality, plasma membrane integrity, and acrosome status were obtained in the neat semen followed by diluted and frozen thaw semen. Further, the process of cryopreservation results in a shift of motility from slow to non-progressive in the post-thaw semen with a significant decrease in the path velocities when compared to neat and diluted semen. Hence, it can be concluded that freezing-thawing process reduces the motility and kinematic characters spermatozoa and may be an important factor affecting the fertilizing ability of spermatozoa resulting in poor conception rate after insemination in goats.

  1. Assessment of motion and kinematic characteristics of frozen-thawed Sirohi goat semen using computer-assisted semen analysis

    Science.gov (United States)

    Anand, Mukul; Yadav, Sarvajeet

    2016-01-01

    Aim: The aim was to determine the motion and kinematics characteristic of frozen-thawed spermatozoa in Sirohi goat using computer-assisted semen analysis. Materials and Methods: A study was carried out in Sirohi buck. Semen collection was made biweekly from each buck with the help of artificial vagina. A total of 12 ejaculates were collected from two bucks (six ejaculates from each buck). Freshly collected semen was pooled and later evaluated. The pooled semen sample was extended with standard glycerolated egg yolk tris extender and later subjected to a process of cryopreservation. The motion and kinematic characteristics of spermatozoa were studied during freez-thawing process. Results: Significantly (p<0.01) higher value of live percent, hypo-osmotic swelling test, and acrosomal integrity were recorded in neat semen followed by diluted and frozen thaw semen. The proportion of spermatozoa showing slow progression were the highest in the neat and diluted semen followed by rapid and non-progressively motile, while a reverse pattern was observed in the frozen thaw semen where the proportion of non-progressively motile spermatozoa were significantly (p<0.01) higher followed by slow and rapid progression. Conclusion: This study showed that the best results for motion, vitality, plasma membrane integrity, and acrosome status were obtained in the neat semen followed by diluted and frozen thaw semen. Further, the process of cryopreservation results in a shift of motility from slow to non-progressive in the post-thaw semen with a significant decrease in the path velocities when compared to neat and diluted semen. Hence, it can be concluded that freezing-thawing process reduces the motility and kinematic characters spermatozoa and may be an important factor affecting the fertilizing ability of spermatozoa resulting in poor conception rate after insemination in goats. PMID:27051209

  2. 1-Methylcyclopropene affects the antioxidant system of apricots (Prunus armeniaca L. cv. Búlida) during storage at low temperature.

    Science.gov (United States)

    Egea, Isabel; Flores, Francisco B; Martínez-Madrid, Maria C; Romojaro, Felix; Sánchez-Bel, Paloma

    2010-03-15

    Apricots (Prunus armeniaca cv. Búlida) were treated with 1 μL L⁻¹ [corrected] 1-methylcyclopropene (1-MCP) immediately after harvest and stored in air at 2 degrees C for 21 days. Antioxidant levels (ascorbic acid and carotenoids), enzymatic antioxidant activities (superoxide dismutase (SOD) and unspecific peroxidase (POX)) and total antioxidant capacity (trolox equivalent antioxidant capacity (TEAC)) were determined. The level of oxidative stress was also established by measuring ion leakage during storage. The changes in the antioxidant potential of apricots were related to the capacity of 1-MCP to increase their commercial life. 1-MCP-treated fruits exhibited higher SOD activity, whereas POX activity was significantly higher only after 21 days at 2 degrees C. Treated fruits also exhibited better retention of ascorbate and carotenoids and higher TEAC during storage. In accordance with these observations, lower ion leakage values were detected in 1-MCP-treated apricots. Taken together, these results suggest that 1-MCP conferred a greater resistance to oxidative stress. This, along with the reduction in ethylene production, could contribute to the increase in commercial life and nutritional value observed in 1-MCP-treated apricots.

  3. Evaluation of five protocols for DNA extraction from leaves of Malus sieversii, Vitis vinifera, and Armeniaca vulgaris.

    Science.gov (United States)

    Aubakirova, K; Omasheva, M; Ryabushkina, N; Tazhibaev, T; Kampitova, G; Galiakparov, N

    2014-02-27

    Leaves of Malus sieversii, Vitis vinifera, and Armeniaca vulgaris contain substantial amounts of secondary metabolites, which limit the high-quality DNA extraction performance. In this study, five extraction protocols were compared for their ability to produce good quality DNA from fresh and dried (with silica gel) leaves of these species. The modified protocol of Dellaporta et al., using polyvinylpyrrolidone to bind the phenolic compounds and a high molar concentration of potassium acetate to inhibit co-precipitation of polysaccharides with DNA, produced the best DNA quality for all species tested. DNA extracted by this method had a 1.77-1.96 A260/280 nm ratio and successful amplification of the 18S ribosomal DNA gene. DNA concentrations of dried leaves were lower than those obtained from fresh leaves, which was likely due to aspects of the drying procedure. All five methods for grapevine produced DNA of obvious better quality from green canes compared to leaves, due to the relatively low content of secondary metabolites in the former. For grapevine and apricot, three methods can be equally used to obtain DNA of good quality: the Doyle and Doyle modified method using CTAB and high concentration of NaCl, the Jobes et al. modified method, and the sodium dodecyl sulfate mini preparation method of Edwards et al. The protocol of Jobes et al. using LiCl for RNA removal showed the best results for most of the M. sieversii samples examined.

  4. Accelerated solvent extraction of carotenoids from: Tunisian Kaki (Diospyros kaki L.), peach (Prunus persica L.) and apricot (Prunus armeniaca L.).

    Science.gov (United States)

    Zaghdoudi, Khalil; Pontvianne, Steve; Framboisier, Xavier; Achard, Mathilde; Kudaibergenova, Rabiga; Ayadi-Trabelsi, Malika; Kalthoum-Cherif, Jamila; Vanderesse, Régis; Frochot, Céline; Guiavarc'h, Yann

    2015-10-01

    Extraction of carotenoids from biological matrices and quantifications remains a difficult task. Accelerated solvent extraction was used as an efficient extraction process for carotenoids extraction from three fruits cultivated in Tunisia: kaki (Diospyros kaki L.), peach (Prunus persica L.) and apricot (Prunus armeniaca L.). Based on a design of experiment (DoE) approach, and using a binary solvent consisting of methanol and tetrahydrofuran, we could identify the best extraction conditions as being 40°C, 20:80 (v:v) methanol/tetrahydrofuran and 5 min of extraction time. Surprisingly and likely due to the high extraction pressure used (103 bars), these conditions appeared to be the best ones both for extracting xanthophylls such as lutein, zeaxanthin or β-cryptoxanthin and carotenes such as β-carotene, which present quite different polarities. Twelve surface responses were generated for lutein, zeaxanthin, β-cryptoxanthin and β-carotene in kaki, peach and apricot. Further LC-MS analysis allowed comparisons in carotenoids profiles between the fruits. Copyright © 2015 Elsevier Ltd. All rights reserved.

  5. Simultaneous Determination of Seven Anthraquinone Aglycones of Crude and Processed Semen Cassiae Extracts in Rat Plasma by UPLC–MS/MS and Its Application to a Comparative Pharmacokinetic Study

    Directory of Open Access Journals (Sweden)

    Rixin Guo

    2017-10-01

    Full Text Available Semen cassiae is the ripe seed of Cassia obtusifolia L. or Cassia tora L. of the family Leguminosae. In traditional Chinese medicine, the two forms of Semen cassiae are raw Semen cassiae (R-SC and parched Semen cassiae (P-SC. To clarify the processing mechanism of Semen cassiae, the pharmacokinetics of R-SC and P-SC extracts were examined. A simple, rapid, sensitive ultra-high-performance liquid chromatography–tandem mass spectroscopy (UPLC-MS/MS method was developed and validated for the simultaneous determination of seven anthraquinone aglycones of Semen cassiae (aurantio-obtusin, obtusifolin, questin, 2-hydroxyemodin-1-methyl-ether, rhein, emodin, 1,2,7-trimethoxyl-6,8-dihydroxy-3-methylanthraquinone to compare the pharmacokinetics of raw and parched Semen cassiae in rat plasma. Compared with the R-SC group, Cmax and AUC0-12 tended to be higher in the P-SC group. In particular, Cmax values for aurantio-obtusin, obtusifolin, questin, 2-hydroxyemodin-1-methyl-ether and rhein were significantly higher in the P-SC group (p < 0.05. Meanwhile, Tmax and MRT0-12 tended to be lower in the P-SC group. Specifically, Tmax for aurantio-obtusin and 2-hydroxyemodin-1-methyl-ether and MRT0-12 for obtusifolin and rhein were significantly higher in the P-SC group (p < 0.05.

  6. Simultaneous Determination of Seven Anthraquinone Aglycones of Crude and Processed Semen Cassiae Extracts in Rat Plasma by UPLC-MS/MS and Its Application to a Comparative Pharmacokinetic Study.

    Science.gov (United States)

    Guo, Rixin; Wu, Hongwei; Yu, Xiankuo; Xu, Mengying; Zhang, Xiao; Tang, Liying; Wang, Zhuju

    2017-10-28

    Semen cassiae is the ripe seed of Cassia obtusifolia L. or Cassia tora L. of the family Leguminosae. In traditional Chinese medicine, the two forms of Semen cassiae are raw Semen cassiae (R-SC) and parched Semen cassiae (P-SC). To clarify the processing mechanism of Semen cassiae, the pharmacokinetics of R-SC and P-SC extracts were examined. A simple, rapid, sensitive ultra-high-performance liquid chromatography-tandem mass spectroscopy (UPLC-MS/MS) method was developed and validated for the simultaneous determination of seven anthraquinone aglycones of Semen cassiae (aurantio-obtusin, obtusifolin, questin, 2-hydroxyemodin-1-methyl-ether, rhein, emodin, 1,2,7-trimethoxyl-6,8-dihydroxy-3-methylanthraquinone) to compare the pharmacokinetics of raw and parched Semen cassiae in rat plasma. Compared with the R-SC group, C max and AUC 0-12 tended to be higher in the P-SC group. In particular, C max values for aurantio-obtusin, obtusifolin, questin, 2-hydroxyemodin-1-methyl-ether and rhein were significantly higher in the P-SC group ( p < 0.05). Meanwhile, T max and MRT 0-12 tended to be lower in the P-SC group. Specifically, T max for aurantio-obtusin and 2-hydroxyemodin-1-methyl-ether and MRT 0-12 for obtusifolin and rhein were significantly higher in the P-SC group ( p < 0.05).

  7. Effect of Prunus armeniaca seed extract on health, survivability, antioxidant, blood biochemical and immune status of broiler chickens at high altitude cold desert

    Directory of Open Access Journals (Sweden)

    Sahil Kalia

    2017-11-01

    Full Text Available Extreme climatic conditions and hypobaric hypoxia at high altitude hinders the growth and productivity of chickens. The present study was carried out to examine the effect of aqueous extract of Prunus armeniaca seeds on health, survivability, antioxidants, plasma biochemical parameters, and immune status of broiler chickens at high altitude. Phytochemical analysis of extract revealed the presence of high phenolics, flavonoids, and carotenoids contents. Before the in vivo study, in vitro efficacy evaluation indicated a significant protective effect of the extract in chicken peripheral blood lymphocytes. For in vivo study, experimental groups include control (fed the basal diet, and treatment T1, T2, T3, T4, T5, and T6 which received an aqueous extract of P. armeniaca in drinking water at concentrations of 100, 150, 200, 300, 400, and 800 mg/kg body weight of chicken respectively, along with basal diet for 42 days. Body weight was significantly increased in all treatment groups as compared to control group and the highest body weight was recorded in T3 group. Higher profit was gained in treatment groups due to lesser mortality in chickens. Moreover, chicken in the treatment groups had significantly higher total antioxidant capacity, free radical scavenging activity, interleukin-2, total protein, albumin, globulin level and lower malondialdehyde, interleukin-6, glucose, cholesterol, triglyceride, ALT and AST level as compared to control group. Results suggest that, P. armeniaca extract at 200 mg/kg body weight of chicken, exhibited the beneficial effect on growth performance and survivability rate of broilers and therefore, could be useful as phytogenic feed additive for broiler chickens at high altitude cold desert.

  8. Collection, analysis and cryopreservation of semen from Malayan ...

    African Journals Online (AJOL)

    The Malayan gaur is mainly distributed in the tropical woodlands of Peninsular Malaysia and Southern Thailand. The aim of this study was to collect, analyze and cryopreserve the semen of wild Malayan gaur. Transrectal massage (TM) and electroejaculation (EEJ) technique was applied in semen collection of the Malayan ...

  9. Semen bacterial flora of Rhode Island Breeder cocks in Zaria ...

    African Journals Online (AJOL)

    The semen used in this study was collected from 77 Rhode Island Breeder cocks reared in battery cages under intensive management from a private farm in Zaria, Kaduna State, Nigeria using the back massage procedure, 27 of the 77 semen samples (35.1%) contained bacterial isolates. None of the samples grew fungi.

  10. Standardinng initial cooling of sheep semen before freezing

    African Journals Online (AJOL)

    sheep semen before freezing. C. Kemp. Animal and Dairy Science Research Institute, Private Bag X2,. Irene, 1675 Republic of South Africa. Received 6 June 1985. A practical and repeatable method for the cooling, during the processing phase, of sheep semen, with the aim of minimizing inter-experiment variation is ...

  11. Intestinal Ischaemia-Reperfusion Injury and Semen Characteristics ...

    African Journals Online (AJOL)

    olayemitoyin

    This study investigates the effect of intestinal ischaemia-reperfusion (IIR) injury on semen characteristics ... Increasing incidence of gastro-intestinal emergencies in sheep and goat are due to difficulties which include .... The semen characteristics of West African dwarf bucks infected with Listeria monocytogenes. Bull. Anim.

  12. Prevalence of Low Sperm Count and Abnormal Semen Parameters ...

    African Journals Online (AJOL)

    elearning

    Abnormal Semen Parameters in Male. Partners of Women Consulting at. Infertility Clinic in Abakaliki, Nigeria. *Ugwuja EI1, Ugwu NC1 and Ejikeme BN2. ABSTRACT. In order to provide an insight to the prevalence of low sperm count and abnormal semen parameters in suspected sub- fertile/infertile men in our environment ...

  13. Between male variation in semen characteristics and preliminary ...

    African Journals Online (AJOL)

    This study is part of an ongoing project on artificial insemination in ostriches. The physical output of neat semen from four ostrich males was investigated and the effect of reconstituting semen with: 1) seminal plasma of the same male (SPS); 2) seminal plasma of another male (SPD), and 3) Dulbecco's Modified Eagles ...

  14. Factors predictive of abnormal semen parameters in male partners ...

    African Journals Online (AJOL)

    Semen analysis has remained useful for investigation of male factor infertility. Objective. To assess the pattern of semen parameters, and predictive factors associated with ... Seminal fluid from the male partners was analysed in the laboratory using ... economic deprivation, social neglect, marital instability, emotional.

  15. Predictions of semen production in ram using phenotypic traits by ...

    African Journals Online (AJOL)

    USER

    2010-07-26

    Jul 26, 2010 ... Concentration of semen production is the most important fertility trait in ram and dimension of testis is a good criterion for ... semen concentration, 184 records were used for training a back propagation ANN system and 8 randomly chosen ... methods can be useful in using input and output variable with high ...

  16. Spermatological Parameters of Extended Rabbit Semen in 5 ...

    African Journals Online (AJOL)

    The in vitro evaluation of spermatological parameters is of great importance in the validation of semen samples to be used in artificial insemination. This study was aimed at evaluating the effect of 5% egg yolk plasma from 5 Nigerian domesticated avian species as a component of semen extender in the preservation of ...

  17. Comparative study on semen characteristics of Kolbroek and Large ...

    African Journals Online (AJOL)

    Administrator

    2011-10-19

    Oct 19, 2011 ... 3Tshwane University of Technology, Department of Animal Sciences, Private Bag X680, Pretoria 0001, South Africa. ... technique. Following semen collection, semen was evaluated for macroscopic and microscopic characteristics. Analysis of variance (ANOVA) was used to test the differences between the ...

  18. Seasonal and cryopreservation impacts on semen quality in boars

    Science.gov (United States)

    Seasonal boar infertility occurs worldwide and contributes to economic loss to the pork industry. The current study evaluated cooled vs cryopreserved semen quality of 11 Duroc boars collected in June (cool season) and August 2014 (warm season). Semen was cooled to 16°C (cooled) or frozen over liquid...

  19. Prostaglandin levels and semen quality in male partners of infertile ...

    African Journals Online (AJOL)

    Objective: To provide data on semen prostaglandins in Nigerian men and relate this to fertility potential as provided by semen analysis results. Design: Prospective study. Setting: Infertility Clinic of Obafemi Awolowo University Teaching Hospitals Complex, Ile-Ife, Nigeria Subjects: All male partners of infertile couples who ...

  20. Physico-chemical characteristics of seed oils extracted from different apricot (Prunus armeniaca L.) varieties from Pakistan

    OpenAIRE

    Alkharfy, K. M.; Ashraf, M.; Anwar, F.; Manzoor, M.

    2012-01-01

    The fruit seed oils from four varieties of apricot (Prunus armeniaca L.), namely, Halmas, Nari, Travet and Charmagzi were analyzed for different physico-chemical characteristics. The oil yield from the apricot seeds (kernels) ranged from 32.23-42.51%, while the protein, fiber and ash contents ranged from 13.21-20.90%, 5.13-9.81% and 2.11-3.89%, respectively. The extracted oils had an average iodine value (g of I/100 g of oil) of 96.4-106.3; density at 24 °C, 0.87-0.93 mg/mL; refractive index ...

  1. Secondary sexual traits and semen characteristic of chicken germline chimeras.

    Science.gov (United States)

    Łukaszewicz, E; Lasoń, M; Kowalczyk, A; Bednarczyk, M

    2018-03-31

    Birds obtained by embryo engineering are used to study embryo development and to produce transgenic birds. As this method of producing birds still generate strong emotions of the public opinion head ornaments, testes and semen characteristics of sex chimera roosters were examined to check whether they differ from chickens obtained by non-manipulated methods. Measurements of head ornaments, testes and semen were correlated with each other. Semen quality factor (SQF) was calculated, as well as the level of fluctuating asymmetry (FA) of bilateral traits (wattles and testes). Positive correlation was found for comb width and wattle length and comb thickness and sperm concentration. Semen characteristics and FA did not exceed the level encounter in other chicken lines. Results obtained indicate that germline chimeras are similar in appearance of secondary sexual traits, and semen and testes characteristics to chickens produced in non-manipulated way. © 2018 Blackwell Verlag GmbH.

  2. A longitudinal analysis of artificial insemination with donor semen.

    Science.gov (United States)

    Sulewski, J M; Eisenberg, F; Stenger, V G

    1978-05-01

    A longitudinal analysis of artificial insemination with donor semen (AID) in 114 consecutive couples revealed a 37% over-all pregnancy rate. The 45 pregnancies occurred in 39 patients, and approximately 90% of these pregnancies occurred within six cycles of AID. Pregnancy rates were higher in the age group 25 to 30, in those who received only fresh semen, and in those with a history of a previous pregnancy. Lower pregnancy rates were observed in patients age 31 or older, in those with pelvic disease, and in those randomly receiving freshly thawed semen or fresh semen. The use of a vaginal device for retaining semen, the use of patient positioning, or the duration of infertility did not appear to affect the success rate. When surgically correctable pelvic disease was treated, a pregnancy rate of 22% was obtained.

  3. Indian story on semen loss and related Dhat syndrome.

    Science.gov (United States)

    Prakash, Om; Kar, Sujit Kumar; Sathyanarayana Rao, T S

    2014-10-01

    India is a country of many religions and ancient cultures. Indian culture is largely directed by the Vedic culture since time immemorial. Later Indian culture is influenced by Buddhism, Islam, and Christianity. Indian belief system carries the footprints of these cultures. Every culture describes human behaviors and an interpretation of each human behavior is largely influenced by the core cultural belief system. Sexuality is an important domain which is colored by different cultural colors. Like other cultures, Indian culture believes "semen" as the precious body fluid which needs to be preserved. Most Indian beliefs consider loss of semen as a threat to the individual. Ancient Indian literature present semen loss as a negative health related event. Dhat syndrome (related to semen loss) is a culture-bound syndrome seen in the natives of Indian subcontinent. This article gathers the Indian concepts related to semen loss. It also outlines belief systems behind problems of Dhat syndrome.

  4. Semen A Altshuler: scientist, mentor, teacher

    International Nuclear Information System (INIS)

    Kochelaev, Boris I

    2011-01-01

    International Conference 'Resonances in Condensed Matter' is devoted to 100 years of the birthday of the Corresponding member of Academy of Sciences of the USSR, Professor of the Kazan University Semen Alexandrovich Altshuler (1911–1983). He is well known by pioneer works on EPR, the prediction and grounds for an existence of the neutron magnetic moment, the prediction and the theory of the acoustic paramagnetic resonance, and as a founder of the Kazan scientific school 'Magnetic radiospectroscopy of condensed matter' (with E K Zavoiskii and B M Kozyrev)

  5. The effect of semen collection method and level of egg yolk on capability of dilution and storage of buck semen

    Directory of Open Access Journals (Sweden)

    N.N. Dhaher

    2013-12-01

    Full Text Available The study was conducted to evaluate the effect of semen collection method for reduction of the deleterious interaction between the enzymes of bulbourethral gland and egg yolk during the dilution and storage of buck semen by three different level of egg yolk. Ten bucks were used in this study; the bucks were divided into two groups (five bucks in each group. Semen samples were collected once a week for four weeks from the bucks in first group using an artificial vagina, and from the animals in second group using an electroejaculator. The collected semen samples were diluted with sodium citrate extender with three different level of egg yolk (5, 10 and 20%. Extend semen samples were stored at 5 °C for three days. Computer assisted sperm analysis and Sperm Class Analyzer® were used for evaluation of the buck semen samples. Sperm motility parameters were evaluated which includes; percentage of motile sperm, percentage of progressive motile sperm, the value of the linear velocity (VSL, the value of the average velocity (VAP, the value of the curvilinear velocity (VCL, and the amplitude of lateral movement of the head (ALH. Results showed that all sperm motility parameters under the different level of egg yolk in semen samples that collected by artificial vagina were significantly higher than those which collected by electroejaculator. The percentage of motile sperm and progressive motile sperm of samples that collected by artificial vagina were higher at 10% of egg yolk, while these motility parameters were higher at 5% of egg yolk for semen samples that collected by electroejaculator. The differences between the two methods of semen collection in VCL and ALH were clear and the values were higher in samples that collected using the artificial vagina. The values of VSL, VAP and VCL of semen samples that collected by artificial vagina were higher at the second day than first day of semen storage under 10% of egg yolk. In conclusion, there are effects

  6. Environmental Exposure to Triclosan and Semen Quality.

    Science.gov (United States)

    Zhu, Wenting; Zhang, Hao; Tong, Chuanliang; Xie, Chong; Fan, Guohua; Zhao, Shasha; Yu, Xiaogang; Tian, Ying; Zhang, Jun

    2016-02-17

    Triclosan (2,4,4'-trichloro-2'-hydroxy-diphenyl ether, TCS) is widely used in personal care, household, veterinary and industrial products. It was considered as a potential male reproductive toxicant in previous in vitro and in vivo studies. However, evidence from human studies is scarce. Our study aims to investigate the relationship between TCS exposure and semen quality. We measured urinary TCS concentrations in 471 men recruited from a male reproductive health clinic. TCS was detected in 96.7% of urine samples, with a median concentration of 0.97 ng (mg·creatinine)(-1) (interquartile range, 0.41-2.95 ng (mg·creatinine)(-1)). A multiple linear regression analysis showed a negative association between natural logarithm (Ln) transformed TCS concentration (Ln-TCS) and Ln transformed number of forward moving sperms (adjusted coefficient β = -0.17; 95% confidence interval (CI) (-0.32, -0.02). Furthermore, among those with the lowest tertile of TCS level, Ln-TCS was negatively associated with the number of forward moving sperms (β = -0.35; 95% CI (-0.68, -0.03)), percentage of sperms with normal morphology (β = -1.64; 95% CI (-3.05, -0.23)), as well as number of normal morphological sperms, sperm concentration and count. Our findings suggest that the adverse effect of TCS on semen quality is modest at the environment-relevant dose in humans. Further studies are needed to confirm our findings.

  7. Semen cryopreservation protocols of Mangalarga Marchador stallions

    Directory of Open Access Journals (Sweden)

    Marcela Leite Candeias

    2012-09-01

    Full Text Available The effect of the utilization of three semen protocols (Inra 82®, Merck Gema and Botu-crio® and two filling techniques (0.25 and 0.50 mL straws in Mangalarga Marchador stallions were studied in this experiment. Sperm parameters were assessed during processing and post-freezing. No interactions between the protocols and type of filling were observed, so they were assessed separately. Sperm parameters were not altered when the extender was added to the centrifugation; however, there was reduction of motility and strength when freezing extenders were added. The Botu-crio® protocol preserved the parameters of total and progressive sperm motility, smoothed path velocity (µm/s, straight line velocity (µm/s, track velocity (µm/s and the average and fast spermatozoa percentage better than the others. No difference between the extenders for the percentage of sperm integrity was observed. There was no difference in the responses studied on the filling techniques. The stallions presented better freezing with the use of the Botu-crio® protocol. The best post-freezing viability results were found for semen frozen using the Botu-crio® protocol and there were no differences concerning the sperm quality comparing 0.25 and 0.50 mL straws.

  8. Effects of Nigella sativa L. seed oil on abnormal semen quality in infertile men: a randomized, double-blind, placebo-controlled clinical trial.

    Science.gov (United States)

    Kolahdooz, M; Nasri, S; Modarres, S Zadeh; Kianbakht, S; Huseini, H Fallah

    2014-05-15

    In recent years, wide utilization of herbal drugs has encouraged scientists to determine their impressive effects on health. Since Nigella sativa L. seed (N. sativa) has many uses including infertility in traditional medicine, the effects of Nigella sativa L. seed oil on abnormal semen quality in infertile men with abnormal semen quality are of interest. This study was conducted on Iranian infertile men with inclusion criteria of abnormal sperm morphology less than 30% or sperm counts below 20×10(6)/ml or type A and B motility less than 25% and 50% respectively. The patients in N. sativa oil group (n=34) received 2.5mlN. sativa oil and placebo group (n=34) received 2.5ml liquid paraffin two times a day orally for 2 months. At baseline and after 2 months, the sperm count, motility and morphology and semen volume, pH and round cells as primary outcomes were determined in both groups. Results showed that sperm count, motility and morphology and semen volume, pH and round cells were improved significantly in N. sativa oil treated group compared with placebo group after 2 months. It is concluded that daily intake of 5ml N. sativa oil for two months improves abnormal semen quality in infertile men without any adverse effects. Copyright © 2014 Elsevier GmbH. All rights reserved.

  9. Semen preservation and artificial insemination in domesticated South American camelids.

    Science.gov (United States)

    Bravo, P Walter; Alarcon, V; Baca, L; Cuba, Y; Ordoñez, C; Salinas, J; Tito, F

    2013-01-10

    Semen preservation and artificial insemination in South American camelids are reviewed giving emphasis to work done in Peru and by the authors. Reports on semen evaluation and the preservation process indicate that semen of alpacas and llamas can be manipulated by making it liquid first. Collagenase appears to be the best enzyme to eliminate viscosity. Tris buffer solution maintains a higher motility than egg-yolk citrate, phosphate buffered saline (PBS), Triladyl, and Merck-I extenders. Cooling of semen took 1h after collected, and equilibrated with 7% glycerol presented a better motility and spermatozoa survival at 1, 7, 15 and 30days after being slowly frozen in 0.25mL plastic straws. Trials of artificial insemination with freshly diluted semen and frozen-thawed semen are encouraging and needs to be tested extensively under field conditions. Recently, fertility rates varied from 3 to 67%. Semen preservation and most important, artificial insemination appear to be a reality, and could be used to improve the genetic quality of alpacas and llamas. Copyright © 2012 Elsevier B.V. All rights reserved.

  10. Vitrification of neat semen alters sperm parameters and DNA integrity.

    Science.gov (United States)

    Khalili, Mohammad Ali; Adib, Maryam; Halvaei, Iman; Nabi, Ali

    2014-05-06

    Our aim was to evaluate the effect of neat semen vitrification on human sperm vital parameters and DNA integrity in men with normal and abnormal sperm parameters. Semen samples were 17 normozoospermic samples and 17 specimens with abnormal sperm parameters. Semen analysis was performed according to World Health Organization (WHO) criteria. Then, the smear was provided from each sample and fixed for terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining. Vitrification of neat semen was done by plunging cryoloops directly into liquid nitrogen and preserved for 7 days. The samples were warmed and re-evaluated for sperm parameters as well as DNA integrity. Besides, the correlation between sperm parameters and DNA fragmentation was assessed pre- and post vitrification. Cryopreserved spermatozoa showed significant decrease in sperm motility, viability and normal morphology after thawing in both normal and abnormal semen. Also, the rate of sperm DNA fragmentation was significantly higher after vitrification compared to fresh samples in normal (24.76 ± 5.03 and 16.41 ± 4.53, P = .002) and abnormal (34.29 ± 10.02 and 23.5 ± 8.31, P neat ejaculates has negative impact on sperm parameters as well as DNA integrity, particularly among abnormal semen subjects. It is, therefore, recommend to process semen samples and vitrify the sperm pellets.

  11. Comparison of four diluents for the retriever dogs semen preservation

    Directory of Open Access Journals (Sweden)

    A. Wicaksono

    2009-03-01

    Full Text Available The quality of chilled semen depends on the composition of diluent. The choice of the buffer, anti-cold shock and nutrition sources can be the first decision in order to choose appropriate diluents. Nowadays a lot of diluent are used for canine semen preservation such as Tris buffer and Citrate buffer. This study was aimed to observe the differences of diluent for preserving Retriever dog spermatozoa. The semen sample collected from four Retriever dogs with three times repetition. The semen was evaluated macro-and microscopically. The semen with >70% sperm motility was divided into four tubes and diluted with diluter 1 (P1, diluter: P2, P3 and P4 (modified P3. The diluted semen was divided into two tubes and each sample was stored at room and 50C temperature. The viability of chilled semen was observed every 3 hours at room temperature and 12 hours at 50C. The result showed that P2 keep the sperm viability better than the other diluents. On 50C at 24 hours storage P2 showed the highest motile and live sperm percentage (46.25 ± 0.22%; 57.11 ± 0.25%. In room temperature at 6 hours P2 showed the highest motile and live sperm percentage (40.94 ± 0.20%; 52.65 ± 0.23%. It is concluded that P2 can keep the sperm viability by 84 hours of 50C and 21 hours at room temperature.

  12. In-vitro free radical scavenging activity of biosynthesized gold and silver nanoparticles using Prunus armeniaca (apricot) fruit extract

    Science.gov (United States)

    Dauthal, Preeti; Mukhopadhyay, Mausumi

    2013-01-01

    In-vitro free radical scavenging activity of biosynthesized gold (Au-NPs) and silver (Ag-NPs) nanoparticles was investigated in the present study. Natural precursor Prunus armeniaca (apricot) fruit extract was used as a reducing agent for the nanoparticle synthesis. The free radical scavenging activity of the nanoparticles were observed by modified 1,1'-diphynyl-2-picrylhydrazyl, DPPH and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid), ABTS assay. The synthesized nanoparticles were characterized by UV-Visible spectroscopy, dynamic light scattering, transmission electron microscopy (TEM), X-ray diffraction (XRD), energy dispersive spectroscopy, and fourier transform infrared spectroscopy (FTIR). Appearance of optical absorption peak at 537 nm (2.20 keV) and 435 nm (3 keV) within 0.08 and 0.5 h of reaction time was confirmed the presence of metallic Au and Ag nanoclusters, respectively. Nearly spherical nanoparticles with majority of particle below 20 nm (TEM) for both Au-NPs and Ag-NPs were synthesized. XRD pattern confirmed the existence of pure nanocrystalline Au-NPs while few additional peaks in the vicinity of fcc silver-speculated crystallization of metalloproteins of fruit extract on the surface of the Ag-NPs and vice versa. FTIR spectra was supported the role of amino acids of protein/enzymes of fruit extract for synthesis and stabilization of nanoparticles. Dose-dependent scavenging activity was observed for Au-NPs and Ag-NPs in both DPPH and ABTS in-vitro assay. 50 % scavenging activity for DPPH were 11.27 and 16.18 mg and for ABTS 3.40 and 7.12 mg with Au-NPs and Ag-NPs, respectively.

  13. In-vitro free radical scavenging activity of biosynthesized gold and silver nanoparticles using Prunus armeniaca (apricot) fruit extract

    Energy Technology Data Exchange (ETDEWEB)

    Dauthal, Preeti; Mukhopadhyay, Mausumi, E-mail: mausumi_mukhopadhyay@yahoo.com [S.V. National Institute of Technology, Department of Chemical Engineering (India)

    2013-01-15

    In-vitro free radical scavenging activity of biosynthesized gold (Au-NPs) and silver (Ag-NPs) nanoparticles was investigated in the present study. Natural precursor Prunus armeniaca (apricot) fruit extract was used as a reducing agent for the nanoparticle synthesis. The free radical scavenging activity of the nanoparticles were observed by modified 1,1 Prime -diphynyl-2-picrylhydrazyl, DPPH and 2,2 Prime -azinobis (3-ethylbenzothiazoline-6-sulfonic acid), ABTS assay. The synthesized nanoparticles were characterized by UV-Visible spectroscopy, dynamic light scattering, transmission electron microscopy (TEM), X-ray diffraction (XRD), energy dispersive spectroscopy, and fourier transform infrared spectroscopy (FTIR). Appearance of optical absorption peak at 537 nm (2.20 keV) and 435 nm (3 keV) within 0.08 and 0.5 h of reaction time was confirmed the presence of metallic Au and Ag nanoclusters, respectively. Nearly spherical nanoparticles with majority of particle below 20 nm (TEM) for both Au-NPs and Ag-NPs were synthesized. XRD pattern confirmed the existence of pure nanocrystalline Au-NPs while few additional peaks in the vicinity of fcc silver-speculated crystallization of metalloproteins of fruit extract on the surface of the Ag-NPs and vice versa. FTIR spectra was supported the role of amino acids of protein/enzymes of fruit extract for synthesis and stabilization of nanoparticles. Dose-dependent scavenging activity was observed for Au-NPs and Ag-NPs in both DPPH and ABTS in-vitro assay. 50 % scavenging activity for DPPH were 11.27 and 16.18 mg and for ABTS 3.40 and 7.12 mg with Au-NPs and Ag-NPs, respectively.

  14. Effects of habitat and season on removal and hoarding of seeds of wild apricot (Prunus armeniaca) by small rodents

    Science.gov (United States)

    Ji-Qi, Lu; Zhi-Bin, Zhang

    2004-12-01

    The wild apricot (Prunus armeniaca) is widely distributed in the Donglingshan Mountains of Mentougou District of Beijing, China, where its seeds may be an important food resource for rodents. Predation, removal and hoarding of seeds by rodents will inevitably affect the spatio-temporal pattern of seed fate of wild apricot in this area. By marking and releasing tagged seeds of wild apricot, we investigated seeds survival, scatter-hoarding, cache size and seedling establishment, and the preference of micro-habitats used by rodents to store seeds. The results showed that: (1) rodents in this area hoarded food intensively in autumn, as well as in spring and summer. (2) There were significant effects of habitat and season on removal rate of tagged seeds at releasing plots. In both two types of habitats, Low and High shrub, tagged seeds were removed most rapidly by rodents in autumn, at intermediate rates in spring and least rapidly in summer. (3) During three seasons, mean dispersal distance of scatter-hoarded seeds in Low shrub habitat was greater than that in High shrub. Most removed seeds were buried within 21.0 m of the releasing plots. (4) In both two types of habitats, Low and High shrub, rodents tended to carry seeds to US (Under shrub) and SE (Shrub edge) microhabitats for scatter-hoarding or predation. (5) Among the caches made by rodents, most caches contained only one seed, but up to three seeds were observed; caches of 2-3 seeds were common in autumn. (6) By comparing dental marks, we determined that large field mice (Apodemus peninsulae) and David's rock squirrels (Sciurotamias davidianus) contributed to removal and predation of released tagged seeds. However, only the large field mice exerted a pivotal and positive role on the burial of dispersed seeds. (7) Establishment of three seedlings originated from seeds buried by rodents was documented in High shrub habitat.

  15. Physico-chemical characteristics of seed oils extracted from different apricot (Prunus armeniaca L.) varieties from Pakistan

    Energy Technology Data Exchange (ETDEWEB)

    Manzoor, M.; Anwar, F.; Ashraf, M.; Alkharfy, K. M.

    2012-11-01

    The fruit seed oils from four varieties of apricot (Prunus armeniaca L.), namely, Halmas, Nari, Travet and Charmagzi were analyzed for different physico-chemical characteristics. The oil yield from the apricot seeds (kernels) ranged from 32.23-42.51%, while the protein, fiber and ash contents ranged from 13.21-20.90%, 5.13-9.81% and 2.11-3.89%, respectively. The extracted oils had an average iodine value (g of I/100 g of oil) of 96.4-106.3; density at 24 degree centigrade 0.87-0.93 mg/mL; refractive index (40 degree centigrade), 1.4655-1.4790; saponification value, 189.1-199.4 mg of KOH/g oil; unsaponifiable matter, 0.59-0.88%; free fatty acid (mg of KOH/g oil), 0.41-1.28; and color (1-inch cell), 1.31-2.96R 1 14.8-29.8Y. With regard to the oxidation state, the tested oils showed values for specific extinction at 232 and 268 nm, 2.30-3.42 and 0.82-1.04, respectively, while the peroxide value was 1.0-2.32 meq O{sub 2}/kg and, p-anisidine was 1.22-1.90. The major fatty acid found in the oils was oleic acid (62.34-80.97%) followed by linoleic (13.13-30.33%), palmitic (3.35-5.93%), linolenic (0.73-1.03%) and stearic (1.10-1.68%) acids. The contents of {alpha}-, {gamma}-, and {delta}-, tocopherols in the oils ranged from 14.8-40.4, 330.8-520.8 and 28.5-60.2 mg/kg, respectively. The results of our present investigation revealed that apricot seed is a potential source of oil which can be used both for edible and oleo chemical applications. (Author) 55 refs.

  16. Changes in the Polyphenolic Profile, Carotenoids and Antioxidant Potential of Apricot (Prunus armeniaca L. Leaves during Maturation

    Directory of Open Access Journals (Sweden)

    Alam Zeb

    2017-02-01

    Full Text Available Apricot (Prunus armeniaca L. leaves were studied to assess the potential of apricot leaves for future studies and their applications in nutraceutical and bioactive functional ingredients. The changes in the phenolic profile, carotenoids, pigments and antioxidant potential were studied at four maturation stages. Polyphenols and carotenoids were studied using reversed-phase HPLC-DAD. Pigments, total phenolic contents and radical scavenging activity were also measured. Results revealed twelve phenolic compounds in the apricot leaves. The major phenolic compounds were 3-O-caffeoylquinic acid (14.6–49.6 mg/g, 4-O-caffeoylquinic acid (0.56–7.5 mg/g, 5-O-caffeoylquinic acid (5.6–25.7 mg/g and quercetin-3-O-glucosides (8.6–19.9 mg/g, while others include caffeic acid and derivatives of coumaric acid and kaempferol. Significant changes were observed in polyphenolic compounds during maturation. Lutein (56.7–65.7 µg/g, neoxanthin (0.66–4.79 µg/g, 5,6-epoxy-α-carotene (5.89–7.9 µg/g, and β-carotene (12.3–26.9 µg/g were the major carotenoids. There were significant variations in the carotenoids, pigment contents, total phenolic contents and radical scavenging activity during maturation. In conclusion, significant variation occurred in the polyphenolic profile, carotenoids contents and antioxidant potential of apricot leaves under the studied conditions.

  17. Relationships between rabbit semen characteristics and fertilising ability after insemination.

    Science.gov (United States)

    Theau-Clément, M; Ailloud, E; Sanchez, A; Saleil, G; Brun, J M

    2016-03-01

    This study aimed to analyse the relationship between rabbit semen characteristics and semen fertilising ability after insemination, which is generally found to be weak. Our hypothesis was that using high semen dilutions (1 : 19), non-oestrus-stimulated does, and homospermic inseminations would make it easier to predict semen fertilising ability. Semen characteristics were evaluated on 275 ejaculates of 128 INRA1001 bucks, distributed into five successive batches. A total of 1970 inseminations were performed. The continuous semen variables were subdivided into three classes of similar size to account for any non-linear relationship between semen characteristics and fertilising ability. Mass motility was divided into two classes according to the presence or absence of waves under microscope observation. Libido, the presence or absence of gel, volume, percentage of progressive sperms, curvilinear velocity, beat frequency of the flagellum, and straightness and linearity of sperm movement did not affect fertility, prolificacy or productivity. It was confirmed that mass motility, estimated by visual observation under the microscope, significantly influenced fertility as well as the percentage of motile and of rapid sperms, and the amplitude of lateral head displacement, estimated by a computer-assisted semen analysis system. To a lesser extent, the percentage of motile cells and of rapid cells significantly influenced prolificacy. Consequently, mass motility and the percentage of motile cells significantly influenced rabbit doe productivity (+1 live births/AI when the semen showed at least a beginning of wave movement, or when the percentage of motile cells was >84%). Interestingly, a gain of 1.5 rabbits was observed when the percentage of rapid cells changed from 64% to 79%, whereas productivity significantly dropped beyond 83% of rapid cells, reflecting a non-linear relationship.

  18. [The effect of stress on the semen quality].

    Science.gov (United States)

    Jurewicz, Joanna; Hanke, Wojciech; Sobala, Wojciech; Merecz, Dorota; Radwan, Michał

    2010-01-01

    Stress is one of the most important health and social problems. Psychological stress has long been suspected of having an important impact on infertility. Studies on the effects of psychological stress on male fertility, especially the semen quality, have so far yielded equivocal findings. The study population consisted of 179 men who had reported to the infertility clinic for diagnostic purposes, some of them with normal fertility (semen total concentration of 15-300 mln/ml) or with slight oligozoospermia (semen total concentration of 10-15 mln/ml). The semen samples were analyzed in one laboratory according to the WHO manual on basic semen analysis. The main semen parameters were assessed: volume, motility, percent of atypical and progressive spermatozoa. To assess the occupational stress the Subjective Work Characteristics Questionnaire was used. Assessing the level of stress we concentrated on the sum of points obtained by each man taking part in the study and the number of psychosocial factors present at work reported by the subjects. Measuring the level of stress by the Subjective Work Characteristics Questionnaire, we revealed negative effects of a number of stressful situations at work, which affect semen volume and percent of progressive spermatozoa, taking account of confounding factors that can have impact on the semen quality and thus affect fertility, such as duration of the couple's infertility, past sexual abstinence and diseases. The study confirm that the occupational stress can affect the male semen quality, however, due to the limited data on this issue, the obtained results must be confirmed by more extensive, longitudinal studies.

  19. Evaluation of sperm chromatin structure in boar semen

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    Banaszewska Dorota

    2015-06-01

    Full Text Available This study was an attempt to evaluate sperm chromatin structure in the semen of insemination boars. Preparations of semen were stained with acridine orange, aniline blue, and chromomycin A3. Abnormal protamination occurred more frequently in young individuals whose sexual development was not yet complete, but may also be an individual trait. This possibility is important to factor into the decision regarding further exploitation of insemination boars. Thus a precise assessment of abnormalities in the protamination process would seem to be expedient as a tool supplementing morphological and molecular evaluation of semen. Disruptions in nucleoprotein structure can be treated as indicators of the biological value of sperm cells.

  20. Bacterial communities in semen from men of infertile couples: metagenomic sequencing reveals relationships of seminal microbiota to semen quality.

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    Shun-Long Weng

    Full Text Available Some previous studies have identified bacteria in semen as being a potential factor in male infertility. However, only few types of bacteria were taken into consideration while using PCR-based or culturing methods. Here we present an analysis approach using next-generation sequencing technology and bioinformatics analysis to investigate the associations between bacterial communities and semen quality. Ninety-six semen samples collected were examined for bacterial communities, measuring seven clinical criteria for semen quality (semen volume, sperm concentration, motility, Kruger's strict morphology, antisperm antibody (IgA, Atypical, and leukocytes. Computer-assisted semen analysis (CASA was also performed. Results showed that the most abundant genera among all samples were Lactobacillus (19.9%, Pseudomonas (9.85%, Prevotella (8.51% and Gardnerella (4.21%. The proportion of Lactobacillus and Gardnerella was significantly higher in the normal samples, while that of Prevotella was significantly higher in the low quality samples. Unsupervised clustering analysis demonstrated that the seminal bacterial communities were clustered into three main groups: Lactobacillus, Pseudomonas, and Prevotella predominant group. Remarkably, most normal samples (80.6% were clustered in Lactobacillus predominant group. The analysis results showed seminal bacteria community types were highly associated with semen health. Lactobacillus might not only be a potential probiotic for semen quality maintenance, but also might be helpful in countering the negative influence of Prevotella and Pseudomonas. In this study, we investigated whole seminal bacterial communities and provided the most comprehensive analysis of the association between bacterial community and semen quality. The study significantly contributes to the current understanding of the etiology of male fertility.

  1. Adherence to reporting guidelines in observational studies concerning exposure to persistent organic pollutants and effects on semen parameters.

    Science.gov (United States)

    Serrano, M; Gonzalvo, M C; Sánchez-Pozo, M C; Clavero, A; Fernández, M F; López-Regalado, M L; Mozas, J; Martínez, L; Castilla, J A

    2014-06-01

    negative effect of exposure to persistent pesticides on sperm concentration presented a lower level of compliance to SEMQUA (42.1 ± 18.3% versus 57.6 ± 14.2%; P observed effect on sperm concentration were the only candidate variables included in the model of stepwise multiple regression model for the 'degree of compliance' variables of SEMQUA and STROBE. Other characteristics of reporting quality, such as legibility, were not evaluated. The low degree of compliance observed is consistent with that observed in other studies of reproductive medicine and highlights the need to improve the design of studies of semen quality. SEMQUA proved to be a more specific tool than STROBE for the field of semen quality. Editors, reviewers and authors should be aware of SEMQUA and apply it when assessing papers on semen quality. No research funding was received and none of the authors have any conflict of interests.

  2. Do perfluoroalkyl compounds impair human semen quality?

    DEFF Research Database (Denmark)

    Joensen, Ulla Nordström; Bossi, Rossana; Leffers, Henrik

    2009-01-01

    in 105 Danish men from the general population (median age, 19 years). RESULTS: Considerable levels of perfluorooctane sulfonic acid (PFOS), perfluorooctanoic acid (PFOA), and perfluorohexane sulfonic acid were found in all young men (medians of 24.5, 4.9, and 6.6 ng/mL, respectively). Men with high...... combined levels of PFOS and PFOA had a median of 6.2 million normal spermatozoa in their ejaculate in contrast to 15.5 million among men with low PFOS-PFOA (p = 0.030). In addition, we found nonsignificant trends with regard to lower sperm concentration, lower total sperm counts, and altered pituitary......-gonadal hormones among men with high PFOS-PFOA levels. CONCLUSION: High PFAA levels were associated with fewer normal sperm. Thus, high levels of PFAAs may contribute to the otherwise unexplained low semen quality often seen in young men. However, our findings need to be corroborated in larger studies....

  3. Association between varicocele grade and semen analysis parameter

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    Ira Ariyati

    2018-02-01

    Full Text Available Background: Varicocele is a condition characterized by elongation, dilatation and tortuousity of spermatic vein in pampiniform plexus. Approximately 50% of infertility cases among men are caused by varicocele. The varicocele may affect the components of sperm. This study aimed to determine the association between varicocele grade based on ultrasound Doppler examination and sperm concentration, sperm motility, and sperm morphology based on semen analysis examination.Methods: This was a descriptive, cross-sectional study which used secondary data from 85 patients that visited Department of Urology, Cipto Mangunkusumo Hospital, then underwent ultrasonography examination at Department of Radiology, Cipto Mangunkusumo Hospital and semen analysis examination at Department of Obstetrics and Gynecology, Cipto Mangunkusumo General Hospital.Results: Varicocele grade was significantly associated with sperm morphology, concentration and motility (all p<0.05. Significant association was found between maximum condition and semen analysis component.Conclusion: Varicocele grade may affect semen analysis component.

  4. Semen Characteristics of the West African Dwarf Rams in The ...

    African Journals Online (AJOL)

    WAD) rams in Makurdi. Semen samples were collected from five rams twice a week by electroejaculation method over a period of two months (September to October). The ejaculate volume, concentration, mass activity, progressive motility, the ...

  5. Indian story on semen loss and related Dhat syndrome

    Science.gov (United States)

    Prakash, Om; Kar, Sujit Kumar; Sathyanarayana Rao, T. S.

    2014-01-01

    India is a country of many religions and ancient cultures. Indian culture is largely directed by the Vedic culture since time immemorial. Later Indian culture is influenced by Buddhism, Islam, and Christianity. Indian belief system carries the footprints of these cultures. Every culture describes human behaviors and an interpretation of each human behavior is largely influenced by the core cultural belief system. Sexuality is an important domain which is colored by different cultural colors. Like other cultures, Indian culture believes “semen” as the precious body fluid which needs to be preserved. Most Indian beliefs consider loss of semen as a threat to the individual. Ancient Indian literature present semen loss as a negative health related event. Dhat syndrome (related to semen loss) is a culture-bound syndrome seen in the natives of Indian subcontinent. This article gathers the Indian concepts related to semen loss. It also outlines belief systems behind problems of Dhat syndrome. PMID:25568479

  6. Environmental Exposure to Triclosan and Semen Quality

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    Wenting Zhu

    2016-02-01

    Full Text Available Triclosan (2,4,4′-trichloro-2′-hydroxy-diphenyl ether, TCS is widely used in personal care, household, veterinary and industrial products. It was considered as a potential male reproductive toxicant in previous in vitro and in vivo studies. However, evidence from human studies is scarce. Our study aims to investigate the relationship between TCS exposure and semen quality. We measured urinary TCS concentrations in 471 men recruited from a male reproductive health clinic. TCS was detected in 96.7% of urine samples, with a median concentration of 0.97 ng (mg·creatinine−1 (interquartile range, 0.41–2.95 ng (mg·creatinine−1. A multiple linear regression analysis showed a negative association between natural logarithm (Ln transformed TCS concentration (Ln-TCS and Ln transformed number of forward moving sperms (adjusted coefficient β = −0.17; 95% confidence interval (CI (−0.32, −0.02. Furthermore, among those with the lowest tertile of TCS level, Ln-TCS was negatively associated with the number of forward moving sperms (β = −0.35; 95% CI (−0.68, −0.03, percentage of sperms with normal morphology (β = −1.64; 95% CI (−3.05, −0.23, as well as number of normal morphological sperms, sperm concentration and count. Our findings suggest that the adverse effect of TCS on semen quality is modest at the environment-relevant dose in humans. Further studies are needed to confirm our findings.

  7. Artificial insemination and cryopreservation of semen from nondomestic birds

    Science.gov (United States)

    Gee, G.F.; Bakst, M.R.; Wishart, G.J.

    1995-01-01

    Studies of Al and cryopreservation of semen from nondomestic birds began because of the increased emphasis on conservation of avian species threatened with extinction. Over the years, aviculturists have developed techniques for Al and cryopreservation of semen obtained from a variety of birds ranging from passerines to Andean condors. Generally, for each new species, we develop a practical semen collection technique and then evaluate the semen. A commercial semen extender (Beltsville Poultry Semen Extender) is modified and used to dilute the semen and provide support for the sperm during the freezing process (the pH and osmolality of the extender is adjusted to reflect the pH and osmolality of the semen being frozen). We find that the freezing schedule developed by Sexton (1977), which utilizes dimethylsulfoxide (DMS0) as cryoprotectant, works well for many species. We cool the sample sequentially in an ethanol bath, in liquid nitrogen vapor, and lastly in liquid nitrogen. Although we have experimented with a variety of freezing protocols, we prefer a 15-min equilibration period in DMSO at 5 C. We begin the freezing process by cooling at -1 C/min from 5 to -20 C in the ethanol bath. The samples are transferred into a vapor tank at a location just above liquid nitrogen and frozen at -50 C/min to -80 C. To complete the freezing process, the samples are plunged into the liquid nitrogen in the bottom of the vapor tank. The samples remain in liquid nitrogen until they are thawed just before insemination. If necessary, the freezing equipment can be transported in a van to remote locations.

  8. Polymyxin B effects on motility parameters of cryopreserved bull semen

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    Mojtaba Rashedi

    2017-01-01

    Full Text Available Objective: To evaluate the effect of adding different values of polymyxin B (PMB to bull semen on various motility parameters of post-thawed semen such as total motility, progressive motility and velocity parameters using kinetic parameters of sperm by Computer Assisted Sperm Analysis. Methods: Gram negative bacteria release lipopolysaccharide, which induces the apoptotic pathway. Antibiotics are added to semen in order to prevent bacterial contaminations in bovine semen. These antibiotics kill the bacteria especially gram negative bacteria. Therefore, their endotoxins are released during bacteriolysis and bind to the head region and midpiece of sperm. PMB is a bactericidal antibiotic against multidrug resistant gram-negative bacteria and is able to neutralize the toxic effects of the released endotoxin. This study was performed on 3-year old Taleshi bulls. Results: The results showed both positive and negative significant effects of PMB on semen quality. Total motility and progressive motility were significantly increased (P<0.000 1 by 100 μg per mL of PMB (55.2% and 48.8% respectively against the control groups (43.5% and 37.7%, respectively. Moreover, they were significantly decreased (P<0.000 1 by 1 000 μg per mL of PMB (35.2% and 28.8% respectively against the control groups (43.5% and 37.7% respectively in above-mentioned parameters. In Computer Assisted Semen Analyzer, parameter VAP was significantly decreased (P<0.04 in 1 000 μg (69.6 μm/s against the control group (78.7 μm/s. Finally, using PMB in processing cryopreserved bull semen is advised, but before using it, the rate of endotoxins must be measured. Conclusions: We advise using PMB after measuring endotoxin concentration; In vitro, in vivo and in field fertilization, adding other sperm evaluation factors such as acrosomal integrity, DNA integrity, mitochondrial function to PMB treated semen.

  9. Semen Displacement as a Sperm Competition Strategy in Humans

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    Gordon G. Gallup

    2004-01-01

    Full Text Available We examine some of the implications of the possibility that the human penis may have evolved to compete with sperm from other males by displacing rival semen from the cervical end of the vagina prior to ejaculation. The semen displacement hypothesis integrates considerable information about genital morphology and human reproductive behavior, and can be used to generate a number of interesting predictions.

  10. Hormonal induction and semen characteristics of tambaqui Colossoma macropomum.

    Science.gov (United States)

    Maria, Alexandre Nizio; Azevedo, Hymerson Costa; Santos, Jadson Pinheiro; Carneiro, Paulo César Falanghe

    2012-02-01

    In the hatchery-bred tambaqui Colossoma macropomum, spontaneous semen release does not occur, and hand-stripping produces reduced semen volume. The goal of this work is to evaluate the effects of hormonal induction with carp pituitary extract (CPE) on both qualitative (visual aspect, pH, motility, viability and morphological abnormalities) and quantitative (volume, concentration and number of spermatozoa per ejaculate) traits of tambaqui semen. Eleven males were treated with CPE (induced), and 11 were left untreated as a control (non-induced). All analysed parameters except motility and percentage of viable spermatozoa presented significant differences (p < 0.05) between the induced and non-induced treatments. CPE induction resulted in a 25-fold increase in semen volume and a 10-fold increase in the number of spermatozoa collected. However, both sperm concentration and the frequency of sperm with morphological abnormalities (commonly detached heads or bent tails) were significantly lower in CPE-induced fish. The hormonal induction of tambaqui males with CPE is efficient and positively influences some qualitative and quantitative properties of semen. Additionally, semen collection via gentle abdominal massage occurs more readily in CPE-induced fish.

  11. Decline of semen quality among Chinese sperm bank donors within 7 years (2008-2014

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    Li Wang

    2017-01-01

    Full Text Available Semen from 5210 sperm bank donors was analyzed and trends in semen quality were evaluated at Shandong Human Sperm Bank between 2008 and 2014. After 2-7 days of abstinence, semen samples were collected. Measurements of semen volume, sperm concentration, sperm forward motility, and total sperm count were performed. There were significant declining trends in semen volume, sperm concentration, sperm forward motility, and total sperm count. Our results indicate that the quality of semen in this cohort of sperm donors had decreased during the study period.

  12. Comparative Examination of Capercaillie (Tetrao urogallus L. Behaviour Responses and Semen Quality to Two Methods of Semen Collection.

    Directory of Open Access Journals (Sweden)

    Ewa Teresa Łukaszewicz

    Full Text Available Artificial insemination (AI is very helpful in solving the reproductive and biodiversity problems observed in small, closed avian populations. The successful production of fertilized eggs using AI is dependent on the collection of good quality semen. Two methods of male sexual stimulation and semen collection from captive kept capercaillie (Tetrao urogallus L., one of the most seriously endangered grouse species in Europe, are compared in this study. Ejaculates were obtained either with the use of a dummy female or by the dorso-abdominal massage method. Differences in the individual responses of the males to the two methods of semen collection as well as in their semen quality were noted. Only sperm concentration (432.4 x 10(6 mL(-1 with dummy female and 614.5 x 10(6 mL(-1 for massage method was significantly affected by capercaillie stimulation method. Sperm motility and morphology were not affected (P ≥ 0.05. Thus, for semen collection from captive kept capercaillie both methods can be used successfully. The dummy female can be an alternative to dorso-abdominal massage method, commonly used for semen collection from domesticated bird species.

  13. SEMEN QUALITY ASSESSMENT OF NEW ZEALAND WHITE RABBIT BUCKS

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    Martyna Błaszczyk

    2013-02-01

    Full Text Available Rabbits have been extensively used as a model for large animals and humans. All the reproduction techniques employed with farm animals can be performed with the low-cost rabbit model, and certain placental membrane characteristics make them especially relevant for studies of human teratology. The purpose of this study was to assess semen quality of New Zealand White rabbits. The material represents semen samples collected from adult rabbits (n=30. The semen was obtained by means of artificial vagina. All samples were analyzed using CASA Sperm VisionTM system. To assessed spermatozoa morphology (the length and the width of head and tail; presence of abnormal spermatozoa we used QuickPhoto Micro system. Received data were statistically analyzed. Our research showed decrease of semen parameters value after one hour storage in 37°C. Correlation analysis showed negative correlation between presence of spermatozoa with separated flagellum and CASA parameters value e.g. motility, progressive motility, DAP, DCL, DSL, VAP, VCL, VSL, ALH and BCF. From among 3000 analyzed spermatozoa 14.2% posed abnormal forms. We observed negative influence of semen storage on its quality. Also negative correlations between all types of tail defect and motility of spermatozoa were detectedRabbits have been extensively used as a model for large animals and humans. All the reproduction techniques employed with farm animals can be performed with the low-cost rabbit model, and certain placental membrane characteristics make them especially relevant for studies of human teratology. The purpose of this study was to assess semen quality of New Zealand White rabbits. The material represents semen samples collected from adult rabbits (n=30. The semen was obtained by means of artificial vagina. All samples were analyzed using CASA Sperm VisionTM system. To assessed spermatozoa morphology (the length and the width of head and tail; presence of abnormal spermatozoa we used Quick

  14. The semen microbiome and its relationship with local immunology and viral load in HIV infection.

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    Cindy M Liu

    2014-07-01

    Full Text Available Semen is a major vector for HIV transmission, but the semen HIV RNA viral load (VL only correlates moderately with the blood VL. Viral shedding can be enhanced by genital infections and associated inflammation, but it can also occur in the absence of classical pathogens. Thus, we hypothesized that a dysregulated semen microbiome correlates with local HIV shedding. We analyzed semen samples from 49 men who have sex with men (MSM, including 22 HIV-uninfected and 27 HIV-infected men, at baseline and after starting antiretroviral therapy (ART using 16S rRNA gene-based pyrosequencing and quantitative PCR. We studied the relationship of semen bacteria with HIV infection, semen cytokine levels, and semen VL by linear regression, non-metric multidimensional scaling, and goodness-of-fit test. Streptococcus, Corynebacterium, and Staphylococcus were common semen bacteria, irrespective of HIV status. While Ureaplasma was the more abundant Mollicutes in HIV-uninfected men, Mycoplasma dominated after HIV infection. HIV infection was associated with decreased semen microbiome diversity and richness, which were restored after six months of ART. In HIV-infected men, semen bacterial load correlated with seven pro-inflammatory semen cytokines, including IL-6 (p = 0.024, TNF-α (p = 0.009, and IL-1b (p = 0.002. IL-1b in particular was associated with semen VL (r(2  = 0.18, p = 0.02. Semen bacterial load was also directly linked to the semen HIV VL (r(2 = 0.15, p = 0.02. HIV infection reshapes the relationship between semen bacteria and pro-inflammatory cytokines, and both are linked to semen VL, which supports a role of the semen microbiome in HIV sexual transmission.

  15. Hepatitis C virus detection in the semen of infected patients

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    Norma de Paula Cavalheiro

    Full Text Available Though HCV infection is a serious public health problem, some aspects of its biology are still not well understood, such as its transmission through seminal fluid and sexual transmission. We looked for HCV in the semen of infected patients. Thirteen patients were included. Semen fractions (seminal plasma, leukocytes and spermatozoa were separated with 45% and 90% Percoll gradients. The HCV-RNA in blood and semen fractions was extracted using the same protocol (AMPLICOR Roche and was detected using the qualitative Roche Amplicor test and by agarose gel electrophoresis, with ethidium bromide staining. The mean age of the patients was 40.7 years. Risk factors for the acquisition of HCV included injectable and inhaled drug use in six (42.8%, blood transfusion in four (28.6%, and no risk factors in four (28.6% patients. Genotype 1 was detected in 62% of the patients, followed by genotype 3 in 23% and genotype 2 in 15%. All blood samples were positive, regardless of the technique used for detection. All semen samples identified by Roche Amplicor and analyzed by agarose gel electrophoresis were negative. Among the 52 semen samples (total and fractions identified by the Roche Amplicor method, 45 (87% were inhibited. A negative result was recorded for one (1.9% total semen sample, one (1.9% leukocyte and four (7.7% seminal plasma fractions. Only one (1.9% sample of the spermatozoon fraction was positive. The results obtained suggested false-negative reactions for the semen samples.

  16. Physico-chemical characteristics of seed oils extracted from different apricot (Prunus armeniaca L. varieties from Pakistan

    Directory of Open Access Journals (Sweden)

    Alkharfy, K. M.

    2012-06-01

    Full Text Available The fruit seed oils from four varieties of apricot (Prunus armeniaca L., namely, Halmas, Nari, Travet and Charmagzi were analyzed for different physico-chemical characteristics. The oil yield from the apricot seeds (kernels ranged from 32.23-42.51%, while the protein, fiber and ash contents ranged from 13.21-20.90%, 5.13-9.81% and 2.11-3.89%, respectively. The extracted oils had an average iodine value (g of I/100 g of oil of 96.4-106.3; density at 24 °C, 0.87-0.93 mg/mL; refractive index (40 °C, 1.4655-1.4790; saponification value, 189.1-199.4 mg of KOH/g oil; unsaponifiable matter, 0.59-0.88%; free fatty acid (mg of KOH/g oil, 0.41-1.28; and color (1-inch cell, 1.31-2.96R 1 14.8-29.8Y. With regard to the oxidation state, the tested oils showed values for specific extinction at 232 and 268 nm, 2.30-3.42 and 0.82-1.04, respectively, while the peroxide value was 1.0-2.32 meq O2/kg and, p-anisidine was 1.22-1.90. The major fatty acid found in the oils was oleic acid (62.34-80.97% followed by linoleic (13.13-30.33%, palmitic (3.35-5.93%, linolenic (0.73-1.03% and stearic (1.10-1.68% acids. The contents of α-, γ-, and δ-, tocopherols in the oils ranged from 14.8-40.4, 330.8-520.8 and 28.5-60.2 mg/kg, respectively. The results of our present investigation revealed that apricot seed is a potential source of oil which can be used both for edible and oleochemical applications.Se han analizado las características físico-químicas de aceites de semillas de frutos de cuatro variedades diferentes de albaricoque, Halmas, Nari, Travet y Charmagzi (Prunus armeniaca L.. La producción de aceites de las semillas de albaricoque (hueso osciló entre 32,23-42,51%, mientras que las proteínas, fibra y cenizas dieron valores de 13,21-20,90%, 5,13-9,81% y 2,11-3,89%, respectivamente. Los aceites extraídos presentaron valores promedio de índice de yodo, de 96,4-106,3 (g de I/100 g de aceite; densidades a 24 °C de 0,87-0,93 mg/mL, índices de refracción (40

  17. Maternal folic acid supplement intake and semen quality in Danish sons

    DEFF Research Database (Denmark)

    Jacobsen, Kristoffer; Ramlau-Hansen, Cecilia Høst; Thulstrup, Ane Marie

    2011-01-01

    To examine whether maternal folic acid supplement intake during pregnancy is related to better semen quality in male offspring.......To examine whether maternal folic acid supplement intake during pregnancy is related to better semen quality in male offspring....

  18. Evolving trends in cryopreservation and parameters influencing semen extender preparation - a prospective review.

    Science.gov (United States)

    Sridharan, T B; Vickram, A S

    2016-01-01

    Cryopreservation is a technique by which, semen can be preserved to subzero temperature, usually at -196° C. The freezing of semen desires vitrification mediators that diminish wreck to the cells (spermatozoan) during the freeze and thaw process. Using cryopreservation, the quality of the semen has been increased in the latest years, by which the achievement rate for the insemination techniques has increased in an agreed way. The area need to be focused is to enhance the quality of the semen extender preparation before cryopreservation. Many researchers are working in the area of cryopreservation of human semen with different semen extenders. Several parameters influence the properties of semen extender essential for better post thaw results. This review is mainly focused on a range of parameters which influence the best semen extender for cryopreservation that includes glycerol and its importance, buffer and novel usage of antimicrobial peptides as antimicrobial agents.

  19. Expected net present value of pure and mixed sexed semen artificial insemination strategies in dairy heifers.

    Science.gov (United States)

    Olynk, N J; Wolf, C A

    2007-05-01

    Sexed semen has been a long-anticipated tool for dairy farmers to obtain more heifer calves, but challenges exist for integrating sexed semen into commercial dairy farm reproduction programs. The decreased conception rates (CR) experienced with sexed semen make virgin heifers better suited for insemination with sexed semen than lactating dairy cows. This research sought to identify when various sexed semen breeding strategies provided higher expected net present value (NPV) than conventional artificial insemination (AI) breeding schemes, indicating which breeding scheme is advisable under various scenarios. Budgets were developed to calculate the expected NPV of various AI breeding strategies incorporating conventional (non-sexed) and sexed semen. In the base budgets, heifer and bull calf values were held constant at $500 and $110, respectively. The percentage of heifers expected to be born after breeding with conventional and sexed semen used was 49.2 and 90%, respectively. Breeding costs per AI were held constant at $15.00 per AI for conventional semen and $45.00 per AI for sexed semen of approximately the same genetic value. Conventional semen CR of 58 and 65% were used, and an AI submission rate was set at 100%. Breeding strategies with sexed semen were assessed for breakeven heifer calf values and sexed semen costs to obtain a NPV equal to that achieved with conventional semen. Breakeven heifer calf values for pure sexed semen strategies with a constant 58 and 65% base CR in which sexed semen achieved 53% of the base CR are $732.11 and $664.26, respectively. Breakeven sexed semen costs per AI of $17.16 and $22.39, compared with $45.00 per AI, were obtained to obtain a NPV equal to that obtained with pure conventional semen for base CR of 58 and 65%, respectively. The strategy employing purely sexed semen, with base CR of both 58 and 65%, yielded a lower NPV than purely conventional semen in all but the best-case scenario in which sexed semen provides 90% of

  20. Anti-gastritis and wound healing effects of Momordicae Semen extract and its active component.

    Science.gov (United States)

    Jung, Kiwon; Chin, Young-Won; Chung, Yoon Hee; Park, Yang Hae; Yoo, Hunseung; Min, Dong Sun; Lee, Bongyong; Kim, Jinwoong

    2013-02-01

    Momordicae Semen, Momordica cochinchinensis Springer (Cucurbitaceae), has long been known to effectively relieve boils, rheumatic pain, and hemorrhoids. In this study, we investigated whether Momordicae Semen extract (MSE) has anti-gastritis effects in various rodent models and also explored possible mechanisms for the gastroprotective effects of MSE. MSE provided remarkable protective effects, comparable to those of rebamipide, in ethanol- and diclofenac-induced acute gastritis. In addition, it has demonstrated protective effect in a Helicobacter pylori-insulted chronic gastritis model. MSE also showed wound healing effect on cutaneous injury of mice and stimulated calcitonin gene-related peptide and somatostatin receptors, which may be related to its anti-gastritis effects. In a single oral dose toxicity study, the approximate lethal dose of MSE was determined at >2000 mg/kg/day. The NOAEL was set to be 2000 mg/kg/day from the repeated oral dose toxicity study. Moreover, momordica saponin I, a major ingredient of MSE, treatment decreased gastric mucosa damage indices in the ethanol- and diclofenac-induced acute gastritis models. The results suggest that MSE could be a promising gastroprotective herbal medicine and momordica saponin I might be used as an active marker compound for MSE.

  1. Longer time interval between semen processing and intrauterine insemination does not affect pregnancy outcome.

    Science.gov (United States)

    Jansen, Charlotte H J R; Elisen, Marc G L M; Leenstra, Cor W; Kaaijk, Eugenie M; van Stralen, Karlijn J; Verhoeve, Harold R

    2017-11-01

    To study whether the pregnancy outcome of intrauterine insemination (IUI) is affected by a longer time interval between semen processing and insemination. Retrospective cohort. Teaching hospital. Couples with subfertility and an indication for IUI over a 10-year period. Insemination performed the day after but within 24 hours of semen collection and processing (delayed insemination) compared with insemination performed immediately after sperm collection and processing (immediate insemination). Ongoing pregnancy rate, defined as a pregnancy confirmed by ultrasound at 10 to 12 weeks of gestation. In total, 1,136 cycles were analyzed. In 77 of 547 couples (14%) an ongoing pregnancy occurred after delayed insemination, and in 77 of 589 couples (13%) an ongoing pregnancy occurred after immediate insemination. Both groups had similar baseline characteristics. After adjustment for confounders, there was no difference in the ongoing pregnancy rate between delayed as compared with immediate insemination (odds ratio 0.89; 95% confidence interval, 0.63-1.25). There is no negative effect on pregnancy rate when IUI of processed sperm is delayed until the next day. This approach allows additional flexibility for couples when the male partner is not available on the day of ovulation, and it allows for a spread of workload in the laboratory. Copyright © 2017 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

  2. Semen analysis under photochemotherapy (PUVA-therapy)

    International Nuclear Information System (INIS)

    Schill, W.B.; Hofmann, C.; Plewig, G.

    1979-01-01

    In 9 male patients with psoriasis vulgaris a semen analysis before and during photochemotherapy with 8-methoxypsoralen and UVA (PUVA) was performed to rule out drug-induced toxic damage of spermatogenesis or impairment of fertility due to scrotal hyperthermia. Two hours after oral application of 40-60 mg 8-methoxypsoralen the patients had been irradiated in UVA high intensity treatment units. PUVA-treatments were performed four times weekly until total body clearing was achieved. For complete remission 13-26 (mean 20.5) PUVA-treatments were necessary. Corresponding total UVA-doses were 35.3 - 191.0 (mean 83.2) Joule/cm 2 . The investigated parameters total motility, progressive motility, spermatozoa density, total spermatozoa count, spermatozoa morphology, and seminal plasma fructose remained unchanged. Only the volume of the ejaculate showed a small decrease during 3 months of therapy. From this pilot study there is no evidence, that PUVA-therapy leads to an impairment of fertility in male patients within their reproductive age. (orig.) [de

  3. The utility of nanowater for ram semen cryopreservation

    Science.gov (United States)

    Murawski, Maciej; Schwarz, Tomasz; Patkowski, Krzysztof; Oszczęda, Zdzisław; Jelkin, Igor; Kosiek, Anna; Gruszecki, Tomasz M; Szymanowska, Anna; Skrzypek, Tomasz; Zieba, Dorota A; Bartlewski, Pawel M

    2015-01-01

    Nanowater (NW; water declusterized in the low-temperature plasma reactor) has specific physicochemical properties that could increase semen viability after freezing and hence fertility after artificial insemination (AI) procedures. The main goal of this study was to evaluate ram semen quality after freezing in the media containing NW. Ejaculates from 10 rams were divided into two equal parts, diluted in a commercially available semen extender (Triladyl®; MiniTüb GmbH, Tiefenbach, Germany) prepared with deionized water (DW) or NW, and then frozen in liquid nitrogen. Semen samples were examined for sperm motility and morphology using the sperm class analyzer system and light microscopy. Cryo-scanning electron microscopy (cryo-SEM) was employed to determine the size of extracellular water crystals in frozen semen samples. Survival time at room temperature, aspartate aminotransferase (AspAT) and alkaline phosphatase (ALP) concentrations post-thawing as well as conception/lambing rates after laparoscopic intrauterine AI of 120 ewes were also determined. There were no significant differences between DW and NW groups in sperm progressive motility (26.4 ± 12.2 and 30.8 ± 12.4%) or survival time (266.6 ± 61.3 and 270.9 ± 76.7 min) after thawing and no differences in the percentages of spermatozoa with various morphological defects before or after freezing. There were, however, differences (P < 0.05) in AspAT (DW: 187.1 ± 160.4 vs. NW: 152.7 ± 118.3 U/l) and ALP concentrations (DW: 2198.3 ± 1810.5 vs. NW: 1612.1 ± 1144.8 U/l) in semen samples post-thawing. Extracellular water crystals were larger (P < 0.05) in ejaculates frozen in NW-containing media. Ultrasonographic examinations on day 40 post-AI revealed higher (P < 0.05) conception rates in ewes inseminated with NW (78.3%) compared with DW semen (58.3%), and the percentages of ewes that carried lambs to term were 73.3% and 45.0% in NW and DW groups, respectively (P

  4. Physically Active Men Show Better Semen Parameters than Their Sedentary Counterparts

    OpenAIRE

    Lalinde-Acevedo, Paula C.; Mayorga-Torres, B. Jose Manuel; Agarwal, Ashok; du Plessis, Stefan S.; Ahmad, Gulfam; Cadavid, Ángela P.; Cardona Maya, Walter D.

    2017-01-01

    Background The quality of semen depends upon several factors such as environment, life style, physical activity, age, and occupation. The aim of this study was to analyze and compare the conventional and functional semen parameters in men practicing vigorous physical activity to those of sedentary men. Materials and Methods In this descriptive cross-sectional study, semen samples of 17 physically active men and 15 sedentary men were collected for analysis. Semen analysis was performe...

  5. Effect of cushioned or single layer semen centrifugation before sex sorting on frozen stallion semen quality.

    Science.gov (United States)

    Mari, G; Bucci, D; Love, C C; Mislei, B; Rizzato, G; Giaretta, E; Merlo, B; Spinaci, M

    2015-04-01

    The aim of this study was to compare the effect of presorting centrifugation (cushioned [CC] or single-layer colloid [SLC]), with simple dilution (SD), on the quality of sex-sorted stallion semen before and after sorting and after freezing and thawing. Four ejaculates from each of two fertile stallions were collected 1 week apart and evaluated for percent total sperm motility (TM), percent viable acrosome-intact sperm (VAI), and DNA quality (percentage of DNA fragmentation index). Freezing caused, independently from CC and SLC treatments, a significant decrease of TM (P < 0.05) and VAI (P < 0.05) in both unsorted and sorted semen. On the other hand, sorting did not impair TM and VAI and, interestingly, improved DNA quality in all treatments only before freezing (28 vs 13, 28 vs 10, 22 vs 7 in SD, CC, and SLC for unsorted vs sorted groups, respectively; P < 0.05); this positive effect was lost in the same samples after freezing and thawing, suggesting that the freezing process reduces the DNA quality of sex-sorted sperm. Our results suggest that CC and SLC are not able to select those spermatozoa that possess a better ability to withstand sperm processing associated with sperm sorting and freezing. Copyright © 2015 Elsevier Inc. All rights reserved.

  6. Occurrence of mycoplasmas in semen samples of birds of prey.

    Science.gov (United States)

    Lierz, M; Hafez, H M

    2008-10-01

    Mycoplasmas are well-known pathogens in a variety of animals. In poultry it is known that some species can be transmitted by semen and infect the uterus of females. As the prevalence of mycoplasmas in birds of prey is very high and artificial insemination is a commonly used technique for reproduction, the possibility of transmission Mycoplasma spp. by contaminated semen in birds of prey was investigated. Isolation of mycoplasmas was possible in five out of 32 (15.6%) semen samples of different bird of prey species. Two additional semen samples were positive for mycoplasma DNA using a Mycoplasma-genus-specific polymerase chain reaction. The isolation of mycoplasmas from a testicular sample indicates the testis as the possible source of contamination. Sequencing of large parts (>90%) of the 16S rRNA gene of the isolated mycoplasmas suggests that all isolates belong to the same species. Alignment of the sequenced products with the 16S rRNA gene of Mycoplasma species in GenBank demonstrated a similarity of 97% to Mycoplasma verecundum, but serological testing by immunobinding assay failed to identify it as such. It is recommended that the semen of donor birds of prey is examined for mycoplasmas before its use in artificial insemination.

  7. Association of Vitamin E with Rapid Thawing on Goat Semen

    Directory of Open Access Journals (Sweden)

    Jurandy Mauro Penitente-Filho

    2014-01-01

    Full Text Available The aim of this study was to evaluate the effects of vitamin E associated with rapid thawing on cryopreserved goat semen. Two bucks were used and eight ejaculates per animal were collected using artificial vagina. Semen was diluted with the following treatments: BIOXCELL (control, BIOXCELL + Equex (sodium lauryl sulphate and BIOXCELL + vitamin E 100 μM. Semen was packaged into 0.25 mL straws and cooled at 5°C for 1 hour. Freezing was performed in liquid nitrogen vapor (−155°C during 15 minutes. Then, the straws were immersed in liquid nitrogen (−196°C. Straws were thawed at 38°C/60 seconds or at 60°C/7 seconds with immediate sperm analysis. Hypoosmotic swelling test was performed adding a 20 μL aliquot of thawed semen to 1 mL of hypoosmotic solution (100 mOsm·Kg−1 followed by incubation during 60 minutes in water bath (38°C. Vitamin E did not affect any studied parameters (P>0.05. Nevertheless, defrosting rate of 60°C/7 seconds improved sperm membrane functional integrity (P<0.05. Current knowledge about goat semen cryopreservation is not sufficient to ensure high post-thawing recovery rates; thus, this study brings important data about using antioxidants and different thawing rates on cryopreservation process.

  8. HIV-1 Populations in Semen Arise through Multiple Mechanisms

    Science.gov (United States)

    Dibben, Oliver; Jabara, Cassandra B.; Arney, Leslie; Kincer, Laura; Tang, Yuyang; Hobbs, Marcia; Hoffman, Irving; Kazembe, Peter; Jones, Corbin D.; Borrow, Persephone; Fiscus, Susan; Cohen, Myron S.; Swanstrom, Ronald

    2010-01-01

    HIV-1 is present in anatomical compartments and bodily fluids. Most transmissions occur through sexual acts, making virus in semen the proximal source in male donors. We find three distinct relationships in comparing viral RNA populations between blood and semen in men with chronic HIV-1 infection, and we propose that the viral populations in semen arise by multiple mechanisms including: direct import of virus, oligoclonal amplification within the seminal tract, or compartmentalization. In addition, we find significant enrichment of six out of nineteen cytokines and chemokines in semen of both HIV-infected and uninfected men, and another seven further enriched in infected individuals. The enrichment of cytokines involved in innate immunity in the seminal tract, complemented with chemokines in infected men, creates an environment conducive to T cell activation and viral replication. These studies define different relationships between virus in blood and semen that can significantly alter the composition of the viral population at the source that is most proximal to the transmitted virus. PMID:20808902

  9. Semen amyloids participate in spermatozoa selection and clearance

    Science.gov (United States)

    Roan, Nadia R; Sandi-Monroy, Nathallie; Kohgadai, Nargis; Usmani, Shariq M; Hamil, Katherine G; Neidleman, Jason; Montano, Mauricio; Ständker, Ludger; Röcker, Annika; Cavrois, Marielle; Rosen, Jared; Marson, Kara; Smith, James F; Pilcher, Christopher D; Gagsteiger, Friedrich; Sakk, Olena; O’Rand, Michael; Lishko, Polina V; Kirchhoff, Frank

    2017-01-01

    Unlike other human biological fluids, semen contains multiple types of amyloid fibrils in the absence of disease. These fibrils enhance HIV infection by promoting viral fusion to cellular targets, but their natural function remained unknown. The similarities shared between HIV fusion to host cell and sperm fusion to oocyte led us to examine whether these fibrils promote fertilization. Surprisingly, the fibrils inhibited fertilization by immobilizing sperm. Interestingly, however, this immobilization facilitated uptake and clearance of sperm by macrophages, which are known to infiltrate the female reproductive tract (FRT) following semen exposure. In the presence of semen fibrils, damaged and apoptotic sperm were more rapidly phagocytosed than healthy ones, suggesting that deposition of semen fibrils in the lower FRT facilitates clearance of poor-quality sperm. Our findings suggest that amyloid fibrils in semen may play a role in reproduction by participating in sperm selection and facilitating the rapid removal of sperm antigens. DOI: http://dx.doi.org/10.7554/eLife.24888.001 PMID:28653619

  10. Is semen quality affected by male body fat distribution?

    Science.gov (United States)

    Fejes, I; Koloszár, S; Szöllosi, J; Závaczki, Z; Pál, A

    2005-10-01

    The aim of this study was to examine the relationship of semen parameters, sexual function-related hormones and waist/hip ratio. Eighty-one selected patients presenting with infertility were examined. Weight, height, waist circumference and hip circumference were measured, and reproduction-related hormone levels were determined. Semen was analysed by conventional methods. Semen volume, sperm concentration, motility, total sperm count, total motile sperm cell number, rapid progressive motile sperm count and reproduction-related hormone levels [follicle-stimulating hormone, luteinizing hormone, prolactin, testosterone, 17beta-oestradiol and sexual hormone-binding globulin (SHBG)]. Significant correlations were found: (i) weight, waist circumference and hip circumference versus testosterone level, SHBG level, and testosterone/17beta-oestradiol ratio; (ii) hip circumference versus sperm concentration; (iii) waist circumference and hip circumference versus sperm count, total motile sperm cell number and rapid progressive motile sperm count; (iv) weight versus total sperm count and total motile sperm cell number; (v) waist circumference and hip circumference versus prolactin level (positively) and SHBG (negatively); (vi) waist circumference and waist/hip ratio versus semen volume. It can be concluded that the waist/hip ratio is correlated with the reproductive hormone levels. Although both the waist circumference and hip circumference correlated with the semen characteristics, the waist/hip ratio did not.

  11. The effects of semen collection on fertility in captive, naturally fertile, sandhill cranes

    Science.gov (United States)

    Chen, G.; Gee, G.F.; Nicolich, Jane M.; Taylor, J.A.

    2001-01-01

    We tested to see if semen collection interferes with fertility in naturally fertile pairs of cranes. We used 12 naturally fertile, Florida sandhill crane (Grus canadensis pratensis) pairs for this study, 6 control and 6 experimental. All pairs had previously produced fertile eggs. Semen was collected on Tuesday mornings and Friday afternoons from 26 February 1993 to 4 June 1993. We used standard artificial insemination methods to collect and to evaluate the semen and spermatozoa. Semen collection had minimal effect on semen quality and semen quantity. Semen volume, sperm density, sperm motility, sperm morphology, sperm viability, sperm number per collection, and male response to semen collection exhibited significant daily variation. Although semen collection began 13 days before the first egg in the experimental group, we did not observe differences in the date of first egg laid or in fertility between experimental and control groups. Also, we observed no statistically significant differences in the interval between clutches or in the percentage of broken eggs between experimental and control groups. However, 4 eggs were broken by adults during the disturbance associated with capturing birds for semen collection. We found that females with mates from which we consistently gathered better semen samples produced fewer fertile eggs than females with sires producing poorer semen samples (r = 0.60). We interpret these results to mean that males that were successfully breeding with their mates had little left at the time of our collection.

  12. Rooster Semen Cryopreservation: Effect of Pedigree Line and Male Age on Post-Thaw Sperm Function

    Science.gov (United States)

    The fertility rates of cryopreserved poultry semen are highly variable and not reliable for use in preservation of commercial genetic stocks. Our objective was to evaluate the cryosurvival of semen from 8 pedigreed layer lines at the onset and end of production. Semen from 160 roosters (20/line) was...

  13. No increased sperm DNA fragmentation index in semen containing human papillomavirus or herpesvirus

    DEFF Research Database (Denmark)

    Kaspersen, Maja Døvling; Bungum, Mona Berger Håkonsen; Fedder, J

    2013-01-01

    It remains unknown whether human papillomaviruses (HPVs) or human herpesviruses (HHVs) in semen affect sperm DNA integrity. We investigated whether the presence of these viruses in semen was associated with an elevated sperm DNA fragmentation index. Semen from 76 sperm donors was examined by a PCR...

  14. The combination of kinetic and flow cytometric semen parameters as a tool to predict fertility in cryopreserved bull semen.

    Science.gov (United States)

    Gliozzi, T M; Turri, F; Manes, S; Cassinelli, C; Pizzi, F

    2017-11-01

    Within recent years, there has been growing interest in the prediction of bull fertility through in vitro assessment of semen quality. A model for fertility prediction based on early evaluation of semen quality parameters, to exclude sires with potentially low fertility from breeding programs, would therefore be useful. The aim of the present study was to identify the most suitable parameters that would provide reliable prediction of fertility. Frozen semen from 18 Italian Holstein-Friesian proven bulls was analyzed using computer-assisted semen analysis (CASA) (motility and kinetic parameters) and flow cytometry (FCM) (viability, acrosomal integrity, mitochondrial function, lipid peroxidation, plasma membrane stability and DNA integrity). Bulls were divided into two groups (low and high fertility) based on the estimated relative conception rate (ERCR). Significant differences were found between fertility groups for total motility, active cells, straightness, linearity, viability and percentage of DNA fragmented sperm. Correlations were observed between ERCR and some kinetic parameters, and membrane instability and some DNA integrity indicators. In order to define a model with high relation between semen quality parameters and ERCR, backward stepwise multiple regression analysis was applied. Thus, we obtained a prediction model that explained almost half (R 2=0.47, Pfertility. Once the accuracy of fertility prediction has been confirmed, the model developed in the present study could be used by artificial insemination centers for bull selection or for elimination of poor fertility ejaculates.

  15. Semen evaluation in the chamois of Abruzzi (Rupicapra pyrenaica ornata).

    Science.gov (United States)

    Carluccio, Augusto; Ponzio, Patrizia; Tosi, Umberto; De Amicis, Ippolito; Robbe, Domenico; Pennelli, Massimiliano; Contri, Alberto

    2007-01-01

    The chamois of Abruzzi (Rupicapra pyrenaica ornata) has been classified as endangered by the World Union for Conservation. The objective of this study was to analyze seasonal differences in the characteristics of various male reproductive organs and in semen quality. The study was conduced during 2004 in the reserve of Lama dei Peligni (Italy) on three chamois males aged between 2 and 5 years. Males were captured during March-May months and October-December months. Various testicular and scrotal measurements were taken and semen was collected using an electroejaculator. Sperm motility pattern was evaluated using computer assisted sperm analyzer, membrane integrity using differential staining and morphology with phase contrast microscopy. Testicular size, sperm motility membrane integrity and the percent of morphological normal spermatozoa were greater during October-December. The authors concluded that sperm characteristics are strongly influenced by season and that semen collected in this period (breeding season) has suitable quality for manipulation and long-term preservation.

  16. Seminal plasma lactoferrin concentrations in normal and abnormal semen samples.

    Science.gov (United States)

    Buckett, W M; Luckas, M J; Gazvani, M R; Aird, I A; Lewis-Jones, D I

    1997-01-01

    Although the iron-chelating protein lactoferrin is secreted by the seminal vesicles, the precise role of lactoferrin in semen is unclear. This study aimed to determine whether there is any association between seminal lactoferrin concentrations and normal and abnormal semen samples with and without leucocytospermia. Lactoferrin concentrations were measured by radial immunodiffusion of semen samples from 368 men attending a regional andrology referral center. Routine seminal analysis, including the presence of leucocytospermia, was also performed. Results showed increased seminal lactoferrin in samples showing oligospermia (13.3 mg/100 ml) and oligoasthenospermia (13.4 mg/100 ml) compared to normospermic samples (11.2 mg/100 ml). There were no significant differences in seminal lactoferrin between normospermic samples and azoospermic samples or asthenospermic samples with normal sperm density. Although there was a trend toward increased lactoferrin concentration with leucocytospermia, this was not significant. Possible causes for raised lactoferrin in association with oligospermia are discussed.

  17. Effect of L-Carnitine Supplementation on Boar Semen Quality

    Directory of Open Access Journals (Sweden)

    E. Jacyno

    2007-01-01

    Full Text Available The effect of addition of L-carnitine on boar semen quality was studied in 5 Pietrain boars at age 1.5 - 2.0 years. The boars received 500 mg of L-carnitine per day for 5 weeks. During this period, their ejaculates were collected once a week and evaluated for quality. The control ejaculates had been collected before the application of L-carnitine. It was found that the addition of L-carnitine to the boars' feed had a positive effect on the quality of boar semen. The total ejaculate volume and sperm-rich fraction volume increased by 11% and 10%, respectively; the total ejaculate sperm count increased by 11.5% (P P < 0.01. Sperm concentration and motility, as well as normal acrosome sperm percentage, did not increase considerably. The positive effect of L-carnitine on boar semen quality was observable as early as after one week of its application.

  18. The Relationship between Occupation and Semen Quality

    Directory of Open Access Journals (Sweden)

    Mohammad Hossein Vaziri

    2011-01-01

    Full Text Available Background: Infertility can be a major concern for couples trying to conceive, and occupationalhazards may constitute a main cause of infertility in men. Studies conducted throughout the worldindicate that physical and chemical hazards in the workplace can have a negative impact on malefertility. The main objective of this study was to determine the frequency of occupational categoriesof men who attended an infertility clinic, and to evaluate the differences in the semen qualityparameters among occupational categories.Materials and Methods: This cross-sectional study was conducted on 1164 males who werereferred to the Infertility Research Center in Tehran for treatment of infertility in order to evaluatethe effects of certain occupations on infertility. The participants were divided into several categoriesaccording to their occupations and evaluated by means of a questionnaire for duration of infertility,BMI, sperm count, percentage of normal sperm morphology and percentages of sperm with class Aand class B motilities. Descriptive statistics, analysis of variance, and correlations were conductedusing SPSS 16.0 for Windows.Results: There were no statistically significant differences in the mean sperm count or spermmorphology between occupational categories. Assessment of the differences in the frequency ofsperm motility classes between occupational categories revealed a significant difference only inthe frequency of sperm with class B motility. The lowest mean percentages of sperm with class Bmotility were seen in those involved in the transportation industry, a finding in agreement with anumber of other researches.Conclusion: Our findings revealed an association between occupation and sperm motility. Sinceour study population was relatively small and in many cases exposures to work hazards were brief,a larger study group must be evaluated in order to support the preliminary results of this study.

  19. Paternity after vasectomy with two previous semen analyses without spermatozoa

    OpenAIRE

    Lucon, Marcos; Lucon, Antonio Marmo; Pasqualoto, Fabio Firmbach; Srougi, Miguel

    2007-01-01

    CONTEXT: The risk of paternity after vasectomy is rare but still exists. Overall failure to achieve sterility after vasectomy occurs in 0.2 to 5.3% of patients due to technical failure or recanalization. The objective of this report was to describe a rare but notable case of proven paternity in which the semen analyses had not given evidence of spermatozoa. CASE REPORT: A 44-year-old vasectomized man whose semen analyses had shown azoospermia became a father four years after sterilization. Bl...

  20. Electron microscopy as a tool for canine semen evaluation

    OpenAIRE

    Jurado, Susana B.; Sarmiento, P.; Stornelli, A.

    2008-01-01

    La evaluación de semen canino puede brindar información importante relacionada con la capacidad fecundante del semen fresco o congelado. El estudio de la morfología espermática en el microscopio óptico provee información limitada sobre la estructura del espermatozoide. Sin embargo, el microscopio electrónico de barrido y el microscopio electrónico de transmisión pueden revelar más detalles de la estructura espermática. Es así que muchos espermatozoides considerados morfológicamente normales a...

  1. SEARCH FOR NEW METHOD FOR FREEZING SEMEN SANTA INES SHEEP

    OpenAIRE

    FÃtima Revia Granja Lima

    2010-01-01

    Three experiments were carried out aiming to evaluate the influence of different factors on parameter of post-thawing semen motility and pregnancy index of rams. The first one showed the influence of extender type (skim milk, HEPES and TRIS), freezing temperature (-79oC, -90oC and -120oC) and final concentration of sperm (600 x 106, 800 x 106 and 1000 x 106 sperm per mL) on semen motility parameters of Santa Ines rams. The second one experiment evaluated the influence of egg yolk typ...

  2. The extent of increase in first calving age as a result of implementing various sexed semen breeding strategies

    Directory of Open Access Journals (Sweden)

    Sahereh Joezy-Shekalgorabi

    2014-01-01

    Full Text Available A deterministic simulation was conducted to assess the effects of sexed semen utilization strategies on age at first calving (AFC. Four different strategies were implemented on dairy heifers: continuous use of conventional semen only (CC, continuous use of sexed semen only (SS, utilization of sexed semen for both the first and second services with conventional semen afterwards (S2, and utilization of sexed semen for the first service with conventional semen afterwards (S1. Results indicated that continuous utilization of sexed semen led to the greatest AFC; however at high conception rates, strategies displayed negligible differences on AFC. Increases in estrus detection rate had the greatest effects on decreasing AFC of the SS scenarios. Negative effect of sexed semen on AFC increased when the effect of low estrus detection rate was combined with low conception rate of sexed semen. Results indicated that in the case of access to sexed semen conception rate, prediction of AFC is possible by quadratic polynomial or exponential equations, depending to the applied breeding strategy. Simultaneous utilization of sexed and conventional semen in a herd did not make a substantial change in AFC when a low percentage of sexed semen was employed. Increasing the contribution of different sexed semen strategies led to higher AFC variation, especially for the SS strategy. AFC of strategies that utilize sexed semen is highly dependent on the conception rate, estrus detection rate and the contribution of sex sorted semen in the total number of inseminations of the heifer herd.

  3. The extent of increase in first calving age as a result of implementing various sexed semen breeding strategies

    Energy Technology Data Exchange (ETDEWEB)

    Joezy-Shekalgorabi, S.; Shadparvar, A. A.; Vries, A. de; Gay, K. D.

    2014-06-01

    A deterministic simulation was conducted to assess the effects of sexed semen utilization strategies on age at first calving (AFC). Four different strategies were implemented on dairy heifers: continuous use of conventional semen only (CC), continuous use of sexed semen only (SS), utilization of sexed semen for both the first and second services with conventional semen afterwards (S2), and utilization of sexed semen for the first service with conventional semen afterwards (S1). Results indicated that continuous utilization of sexed semen led to the greatest AFC; however at high conception rates, strategies displayed negligible differences on AFC. Increases in estrus detection rate had the greatest effects on decreasing AFC of the SS scenarios. Negative effect of sexed semen on AFC increased when the effect of low estrus detection rate was combined with low conception rate of sexed semen. Results indicated that in the case of access to sexed semen conception rate, prediction of AFC is possible by quadratic polynomial or exponential equations, depending to the applied breeding strategy. Simultaneous utilization of sexed and conventional semen in a herd did not make a substantial change in AFC when a low percentage of sexed semen was employed. Increasing the contribution of different sexed semen strategies led to higher AFC variation, especially for the SS strategy. AFC of strategies that utilize sexed semen is highly dependent on the conception rate, estrus detection rate and the contribution of sex sorted semen in the total number of inseminations of the heifer herd. (Author)

  4. Dry Orgasm (Orgasm without Discharge of Semen)

    Science.gov (United States)

    ... and predicting factors for commonly neglected sexual side effects to external-beam radiation therapy for prostate cancer. The Journal of Sexual Medicine. 2017;14:558. Hoffman RM, et al. Microwave thermotherapy for benign prostatic hyperplasia. Cochrane Database of ...

  5. New methods and media for the centrifugation of honey bee (Hymenoptera: Apidae) drone semen.

    Science.gov (United States)

    Wegener, Jakob; May, Tanja; Kamp, Günter; Bienefeld, Kaspar

    2014-02-01

    Centrifugation of Apis mellifera L. drone semen is a necessary step in the homogenization of semen pools for the enlargement of the effective breeding population, as well as in the collection of semen by the so-called washing technique. It is also of interest for the removal of cryoprotectants after cryopreservation. The adoption of methods involving semen centrifugation has been hampered by their damaging effect to sperm. Here, we tested four new diluents as well as three additives (catalase, hen egg yolk, and a protease inhibitor), using sperm motility and dual fluorescent staining as indicators of semen quality. Three of the new diluents significantly reduced motility losses after centrifugation, as compared with the literature standard. Values of motility and propidium iodide negativity obtained with two of these diluents were not different from those measured with untreated semen. The least damaging diluent, a citrate-HEPES buffer containing trehalose, was then tested in an insemination experiment with centrifuged semen. Most queens receiving this semen produced normal brood, and the number of sperm reaching the storage organ of the queen was not significantly different from that in queens receiving untreated semen. These results could improve the acceptance of techniques involving the centrifugation of drone semen. The diluent used in the insemination experiment could also serve as semen extender for applications not involving centrifugation.

  6. Lack of impact of semen quality on fertilization in assisted conception.

    Science.gov (United States)

    Kini, S; Morrell, D; Thong, K J; Kopakaki, A; Hillier, S; Irvine, D S

    2010-02-01

    Defective semen quality is one of the commonest causes of infertility. The diagnosis of male fertility depends upon a descriptive evaluation of human semen, however a normal semen analysis does not necessarily indicate satisfactory fertility potential. (i) to examine the semen quality of patients undergoing treatment by assisted conception, (ii) to explore relationships between semen quality and treatment outcomes, and (iii) to look at inter-laboratory variation in the assessment of semen quality. Semen quality in patients undergoing assisted conception treatment between 2001 and 2004 was reviewed. Data on female age, egg numbers and fertilization outcomes was obtained by case note review. The thresholds used to direct patients towards IVF or ICSI treatment were comparable with the normal values promulgated by WHO, with the exception of morphology. Semen quality was not predictive of fertilization rates. When the results of independent measurements of the same sample were compared, there was diagnostic disagreement in between 10%-29% of samples. The conventional criteria of semen quality are used to determine treatment strategy for couples undergoing assisted conception but are not reflected in fertilization rates, emphasising the limited utility of the conventional criteria of semen quality in the assessment of sperm function. There remains significant inter-laboratory variation in the results of semen analysis.

  7. Maintaining semen quality by improving cold chain equipment used in cattle artificial insemination

    Science.gov (United States)

    Lieberman, Daniel; McClure, Elizabeth; Harston, Stephen; Madan, Damian

    2016-06-01

    Artificial insemination of dairy cattle is a common practice in the developing world that can improve farmer incomes and food security. Maintaining the fertilizing potential of frozen semen as it is manipulated, transported and stored is crucial to the success of this process. Here we describe simple technological improvements to protect semen from inadvertent thermal fluctuations that occur when users mishandle semen using standard equipment. We show that when frozen semen is mishandled, characteristics of semen biology associated with fertility are negatively affected. We describe several design modifications and results from thermal performance tests of several improved prototypes. Finally, we compare semen that has been mishandled in standard and improved equipment. The data suggest that our canister improvements can better maintain characteristics of semen biology that correlate with fertility when it is mishandled.

  8. Reproduction in nondomestic birds: Physiology, semen collection, artificial insemination and cryopreservation

    Science.gov (United States)

    Gee, G.F.; Bertschinger, H.; Donoghue, A.M.; Blanco, J.; Soley, J.

    2004-01-01

    Pioneering work by Quinn and Burrows in the late 1930s led to successful artificial insemination (AI) programs in the domestic poultry industry. A variety of species specific modifications to the Quinn and Burrows massage technique made AI possible in nondomestic birds. Massage semen collection and insemination techniques span the entire range of species from sparrows to ostriches. Also, cooperative semen collection and electroejaculation have found limited use in some nondomestic species. Artificial insemination produces good fertility, often exceeding fertility levels in naturally copulating populations. However, aviculturists should explore other ways to improve fertility before resorting to AI. Artificial insemination is labor intensive and may pose risks to nondomestic birds as well as handlers associated with capture and insemination. Semen collection and AI makes semen cryopreservation and germ plasma preservation possible. Yet, semen cryopreservation techniques need improvement before fertility with frozen-thawed semen will equal fertility from AI with fresh semen.

  9. Thermotemporal dynamics of contaminant bacteria and antimicrobials in extended porcine semen.

    Science.gov (United States)

    Althouse, G C; Pierdon, M S; Lu, K G

    2008-11-01

    Bacterial contamination of extended porcine semen has been associated with deleterious effects on both semen quality and sow fertility. Retrospective, prospective and in vitro studies were performed to delineate the prevalence and behavior of certain bacterial contaminants in extended semen, and antimicrobial pharmacodynamics in various semen diluents. Retrospective review of extended semen samples submitted from North American boar studs for microbiological screening at the University of Pennsylvania Reference Andrology Laboratory in 2005 and 2006 yielded bacteriospermia prevalence rates of 17% (144/832) and 26% (256/984), respectively. In a prospective study of regional boar studs, of 91 extended semen samples tested over 1-y, 29% were positive for bacteriospermia. Retrospective and prospective studies both showed that the preponderance of contaminant positive samples occurred during the fall months (Ptylosin, and lincomycin/spectinomycin (single drug or combination) ranged from 75 to over 360min, and was highly dependent (P<0.05) upon both type of bacteria and semen diluent.

  10. The Quality of Frozen-thawed Canine Semen With Respect to Semen Extender Composition and Sequence of Ejaculate Collection in Dogs

    Directory of Open Access Journals (Sweden)

    Jiří Šichtař

    2016-01-01

    Full Text Available The aim of this study was to evaluate the effect of clarified egg yolk addition to semen extender, and the semen collection sequence on the quality of frozen-thawed semen in dogs. Semen was collected from 6 dogs in a time interval of 24 hours. As parameter of the quality of frozen-thawed (F-T semen, the motility by computer assisted sperm analysis (CASA and plasma membrane integrity by hypo-osmotic swelling test (HOS were evaluated. All kinematic parameters of sperm motility were higher in F-T samples containing the whole in comparison to the clarified egg yolk. The sequence of semen collection affected sperm movement characteristics of native as well as F-T semen, but it was not possible to determine whether the fresh semen from the 1st or 2nd collection is of higher quality. All motility parameters of sperms frozen with extender containing the whole egg yolk were significantly higher in the case of the 2nd collection. The situation was not so clear in the case of clarified egg yolk addition, but the velocity values were higher in F-T samples from the 2nd collection. In contrast to proven differences in motility, the effect of the addition of clarified egg yolk and the sequence of semen collection were not projected at all on the quality of plasma membrane of canine sperms evaluated by HOS test.

  11. Effect of antimicrobial-containing semen extender on risk of dissemination of contagious equine metritis.

    Science.gov (United States)

    Klein, Claudia; Donahue, James M; Sells, Steve F; Squires, Edward L; Timoney, Peter J; Troedsson, Mats H T

    2012-10-01

    To determine the impact of antimicrobial-containing semen extender on the growth of Taylorella equigenitalis in semen culture-positive for contagious equine metritis (CEM) and the development of CEM after artificial insemination with CEM-positive semen extended with antimicrobial-containing semen extender. Prospective study. 21 mature mares free of CEM, 1 mature stallion experimentally infected with CEM, and semen from a stallion naturally infected with CEM. CEM-positive semen was incubated in semen extender with and without antimicrobials (amikacin [final concentration, 1 g/L] and penicillin G potassium [0.63 g/L]) followed by determination of the number of colony-forming units of T equigenitalis. Mares were inseminated with raw, extended, or cryopreserved semen culture-positive for T equigenitalis and observed for clinical signs of CEM. Samples for bacterial culture were obtained from the uterus, clitoral sinuses, and clitoral fossa of mares 7, 14, and 21 days after artificial insemination. Antimicrobial-containing semen extender significantly reduced the number of colony-forming units of T equigenitalis in CEM-positive semen. Artificial insemination with raw CEM-positive semen resulted in clinical signs of CEM, whereas artificial insemination with extended or cryopreserved CEM-positive semen did not result in clinical signs of CEM. Antimicrobial-containing semen extender significantly reduced the risk of dissemination of CEM. The inclusion of amikacin (1 g/L) and penicillin G potassium (0.63 g/L) in extended semen reduced the transmission of CEM from stallions to mares during artificial insemination, which may result in altered dissemination of the disease.

  12. Influence of addition of different antibiotics in semen diluent on viable bacterial count and spermatozoal viability of Awassi ram semen

    Directory of Open Access Journals (Sweden)

    O I Azawi

    2012-04-01

    Full Text Available The objectives of the present study were to determine the effects of six different antibiotics in controlling the growth of semen contaminating bacteria and if these antibiotics have any adverse effect on Awassi ram spermatozoa. Semen samples from six mature Awassi rams were used in this study. A total number of 120 ejaculates were collected from the rams using an artificial vagina once a week. Semen ejaculates were evaluated for volume, sperm concentration, mass motility, individual motility, percentage live sperm, sperm abnormalities, and viable bacterial count. Semen samples were diluted by sodium citrate-fructose-egg yolk. The diluted semen sample was divided into 7 parts. Six types of antibiotics were added to the semen diluent parts including; penicillin G 1000 IU ml-1 with streptomycin 1 mg ml-1, gentamicin sulphate 250 mg ml-1, tetracycline 0.5 mg ml-1, lincomycin 1 mg ml-1, cefoperazone sodium 1mg ml-1, cefdinir 1 mg ml-1 and the seventh part considered as a control group without antibiotic addition. The diluted semen samples were cooled and preserved at 5 Co for 5 days. Cooled diluted semen samples were examined for individual motility, percent of live sperm, sperm abnormalities, acrosomal defects and bacterial count every 24 h until 5 days. Comparing with the control, all the antibiotics examined were effective in controlling bacterial growth (P<0.05 from 24 h to 96 h of preservation at 5 Co. Cefdinir and cefoperazone sodium proved to be significantly (P<0.05 effective than other antibiotics in controlling bacterial growth at 96 h of preservation as the bacterial count were 23.3 ± 3.7 x 103 / ml and 25.4 ± 6.2 x 103 / ml, respectively. Lincomycin, gentamicin sulphate and tetracycline proved ineffective in controlling bacterial growth at 96 h of preservation as the bacterial count were 57.1 ± 20.1 x 103 / ml, 52.5 ± 29.4 x 103 / ml and 46.5 ± 8.8 x 103 / ml, respectively. The addition of tetracycline to diluted ram semen

  13. Plastic Straws for Frozen Semen (A Field Evaluation)

    Science.gov (United States)

    Macpherson, J. W.; King, G. J.

    1966-01-01

    A field trial was conducted to compare the routine frozen semen methods presently used at this laboratory with those reported by French workers. When the extender used was sterile milk both methods resulted in fertility equal to the standard. Fertility results obtained with the French method employing Lactiphos extender were significantly lower than the standard. ImagesFig. 1. PMID:4225285

  14. Effects of quantitative feed restriction on the semen quality of ...

    African Journals Online (AJOL)

    The feeding trials lasted for 8 weeks during which two ejaculates per week were collected from each cock using the massage technique. Ejaculated semen samples were subjected to both physical and biochemical evaluations. Results showed that cocks that were severely underfed (i.e. Groups IV and V) took significantly ...

  15. The influence of boar breed and season on semen parameters

    African Journals Online (AJOL)

    Kamil

    2014-01-29

    Jan 29, 2014 ... Abstract. The aim of the present study was to demonstrate the influence of boar breed and season on semen parameters. The research material consisted of 31 boars: Polish Large White (PLW), Polish Landrace (PL), and Duroc × Pietrain (D × P), aged 8 to 24 months. The analysed material consisted of ...

  16. Twin pregnancy possibly associated with high semen quality

    DEFF Research Database (Denmark)

    Asklund, Camilla; Jensen, Tina Kold; Jørgensen, Niels

    2007-01-01

    BACKGROUND: Recent studies found an association between a long waiting time to pregnancy (TTP) and reduced probability of twinning and a reduced dizygotic (DZ) twinning rate in subfertile men. However, it remains unsolved whether semen quality is associated with twin offspring. We therefore studied...

  17. The effects of three extenders on refrigerated boar semen

    African Journals Online (AJOL)

    sofiateixeira

    2015-03-19

    Mar 19, 2015 ... This study aims to evaluate the quality of boar sperm that was refrigerated for 14 days at 17 ºC with three extenders. ... preferred method of storage for boar semen that is to be used for artificial insemination (AI) (Johnson et al.,. 2000). Pursel et ..... Boar sperm viability after freezing and thawing. Proc. 1st Int.

  18. Plastic straws for frozen semen. (A field evaluation).

    Science.gov (United States)

    Macpherson, J W; King, G J

    1966-04-01

    A field trial was conducted to compare the routine frozen semen methods presently used at this laboratory with those reported by French workers. When the extender used was sterile milk both methods resulted in fertility equal to the standard. Fertility results obtained with the French method employing Lactiphos extender were significantly lower than the standard.

  19. Y chromosome TSPY copy numbers and semen quality

    NARCIS (Netherlands)

    Nickkholgh, Bita; Noordam, Michiel J.; Hovingh, Suzanne E.; van Pelt, Ans M. M.; van der Veen, Fulco; Repping, Sjoerd

    2010-01-01

    Objective: To determine whether variation in testis-specific protein Y-encoded (TSPY) gene copy number affects semen quality. Design: Nested case-control study. Setting: University hospital. Patient(s): From a consecutive cohort of 1,016 male partners of subfertile couples, unselected for sperm

  20. Preservation of Domesticated Honey Bee (Hymenoptera: Apidae) Drone Semen.

    Science.gov (United States)

    Paillard, M; Rousseau, A; Giovenazzo, P; Bailey, J L

    2017-08-01

    Preservation of honey bee (Apis mellifera L., Hymenoptera: Apidae) sperm, coupled with instrumental insemination, is an effective strategy to protect the species and their genetic diversity. Our overall objective is to develop a method of drone semen preservation; therefore, two experiments were conducted. Hypothesis 1 was that cryopreservation (-196 °C) of drone semen is more effective for long-term storage than at 16 °C. Our results show that after 1 yr of storage, frozen sperm viability was higher than at 16 °C, showing that cryopreservation is necessary to conserve semen. However, the cryoprotectant used for drone sperm freezing, dimethyl sulfoxide (DMSO), can harm the queen and reduce fertility after instrumental insemination. Hypothesis 2 was that centrifugation of cryopreserved semen to reduce DMSO prior to insemination optimize sperm quality. Our results indicate that centrifuging cryopreserved sperm to remove cryoprotectant does not affect queen survival, spermathecal sperm count, or sperm viability. Although these data do not indicate that centrifugation of frozen-thawed sperm improves queen health and fertility after instrumental insemination, we demonstrate that cryopreservation is achievable, and it is better for long-term sperm storage than above-freezing temperatures for duration of close to a year. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  1. Semen Parameters in Infertile Nigerian Males: A critical Study ...

    African Journals Online (AJOL)

    Introduction: Approximately one couple in ten are infertile during their reproductive lives. Male factors alone are responsible for infertility in 24% of couples and contributed to the cause in another 24%. The most important male factor is the semen parameter. Seminal fluid analysis is therefore the cornerstone of male infertility ...

  2. Semen-like urethral discharge during the use of mazindol

    NARCIS (Netherlands)

    van Puijenbroek, E P; Meyboom, R H

    Two case reports are described of male patients experiencing a semen-like urethral discharge during micturition, suspected to be induced by mazindol. Mazindol has an indirect sympathomimetic action and is known to cause urogenital side effects such as urinary retention and testicular pain. It is

  3. The effects of three extenders on refrigerated boar semen

    African Journals Online (AJOL)

    sofiateixeira

    2015-03-19

    Mar 19, 2015 ... Ambrogi, M., Ballester, J., Saravia, F., Caballero, I., Johannisson, A., Wallgren, M., Andersson, M. &. Rodriguez-Martinez, H., 2006. Effect of storage in short and long term commercial semen extenders on the motility, plasma membrane and chromatin integrity of boar spermatozoa. Int. J. Androl. 29,. 543-552.

  4. Variation of semen parameters in healthy medical students due to ...

    African Journals Online (AJOL)

    Aim. This study was aimed at investigating semen parameters that vary most in samples of healthy donors undergoing stressful examination period. Methods Samples were left to liquefy in an incubator at 37°C, 5% CO2 for 30 minutes before volume was measured. Concentration and motility parameters were measured by ...

  5. Lambing results obtained with imported Ile de France rarn semen

    African Journals Online (AJOL)

    Two batches of lle de France ram semen frozen in 0,5 ml straws were imported from France during April 1929 and. September 198'1. Ewes were synchronized and artificial insemination was carried out either 54,5 h (fixed time single insemination) after sponge withdrawal, or with a doubre insemination, 12 h after the onset ...

  6. Carnitine supplement: Does it worth trial to improve semen quality ...

    African Journals Online (AJOL)

    Carnitine supplement proves to upgrade the quality of semen by increasing sperm count and motility. In this study we have determined the level of L – carnitine in the seminal plasma of men with normal and abnormal seminal analysis. L – carnitine levels among the normal group was significantly higher than the abnormal ...

  7. Association between GH encoding gene polymorphism and semen ...

    African Journals Online (AJOL)

    user

    2011-02-07

    Feb 7, 2011 ... Nonetheless, both impacts are economically important factors which are considered by the producers leading to rejection of bulls with poor semen quality and investing mainly on highly fertile sires. (Januskauskas et al., 1996; Correa et al., 1997;. Rodriguez-Martinez, 2003). Therefore, understanding the ...

  8. Association between GH encoding gene polymorphism and semen ...

    African Journals Online (AJOL)

    The objective of this present study was to investigate relationships between the growth hormone gene restriction fragment length polymorphism (RFLP) and bull sperm characteristics. A total of 89 bulls from two semen evaluation stations were genotyped for the bovine growth hormone (bGH)-AluI polymorphism by ...

  9. incidence of sexually transmitted diseases amongst potential semen

    African Journals Online (AJOL)

    drclement

    need to educate and enlighten our youths on the danger of acquiring these pathogen. Consistent with previous studies this finding strengthens the fact that. Prospective Semen Donors, most of whom are young, single, and are not always in monogamous relationships, represent a high-risk population for STD transmission.

  10. Beneficial effects of semen purification with magnetic nanoparticles

    Science.gov (United States)

    Current techniques for sperm quality evaluation are mostly informative. They become useful when ejaculates of high index males not meeting quality standard are still discarded. Here we developed a molecular-based magnetic conjugates allowing selective elimination of damaged spermatozoa from semen ej...

  11. Semen characteristics, gonadal and extra-gonadal traits of dutch ...

    African Journals Online (AJOL)

    The rabbits were randomly assigned to four experimental diets each supplemented with zinc at 0 ppm/kg (control), 50 ppm/kg, 100 ppm/kg and 150 ppm/kg of feed and designated as Treatments 1, 2, 3 and 4. The feeding trial lasted for eighty-four days in a completely randomized design. Semen sample was collected from ...

  12. Semen quality, biochemistry and mineral content of five strains of ...

    African Journals Online (AJOL)

    The study was conducted to evaluate the genetic effect on semen quality, biochemistry and mineral content of three strains of Nigerian indigenous and two exotic cocks. One hundred (100) adult local breeding cocks comprising 20 normal, 20 frizzle and 20 naked necks, 20 dominant black and 20 dominant blue feather were ...

  13. Fertility chip, a point-of-care semen analyser

    NARCIS (Netherlands)

    Segerink, Loes Irene

    2011-01-01

    Before assistive reproductive treatment will be started for a couple that is childless by default, the cause of the fertility disorder needs to be investigated for both the man as well as the woman. For the man this implies that the quality of his semen needs to be known. Currently, at the hospital

  14. Semen-like urethral discharge during the use of mazindol.

    Science.gov (United States)

    van Puijenbroek, E P; Meyboom, R H

    1998-07-01

    Two case reports are described of male patients experiencing a semen-like urethral discharge during micturition, suspected to be induced by mazindol. Mazindol has an indirect sympathomimetic action and is known to cause urogenital side effects such as urinary retention and testicular pain. It is suggested that seminal discharge may be added to this list.

  15. Study on extraction process of tannins from Semen cuscutae and ...

    African Journals Online (AJOL)

    The objective of this paper was to study the extraction methods of tannin constituents from Semen Cuscutae and their anti-papilloma effects. Single factor test and orthogonal design methods were used to determine the optimal extraction method; the mouse skin papilloma model induced by DMBA/croton oil was established, ...

  16. The influence of boar breed and season on semen parameters ...

    African Journals Online (AJOL)

    The aim of the present study was to demonstrate the influence of boar breed and season on semen parameters. The research material consisted of 31 boars: Polish Large White (PLW), Polish Landrace (PL), and Duroc × Pietrain (D × P), aged 8 to 24 months. The analysed material consisted of 1390 ejaculates, collected ...

  17. Semen analysis and sperm function tests: How much to test?

    Directory of Open Access Journals (Sweden)

    S S Vasan

    2011-01-01

    Full Text Available Semen analysis as an integral part of infertility investigations is taken as a surrogate measure for male fecundity in clinical andrology, male fertility, and pregnancy risk assessments. Clearly, laboratory seminology is still very much in its infancy. In as much as the creation of a conventional semen profile will always represent the foundations of male fertility evaluation, the 5th edition of the World Health Organization (WHO manual is a definitive statement on how such assessments should be carried out and how the quality should be controlled. A major advance in this new edition of the WHO manual, resolving the most salient critique of previous editions, is the development of the first well-defined reference ranges for semen analysis based on the analysis of over 1900 recent fathers. The methodology used in the assessment of the usual variables in semen analysis is described, as are many of the less common, but very valuable, sperm function tests. Sperm function testing is used to determine if the sperm have the biologic capacity to perform the tasks necessary to reach and fertilize ova and ultimately result in live births. A variety of tests are available to evaluate different aspects of these functions. To accurately use these functional assays, the clinician must understand what the tests measure, what the indications are for the assays, and how to interpret the results to direct further testing or patient management.

  18. Implementation of an office-based semen preparation method (SEP ...

    African Journals Online (AJOL)

    Implementation of an office-based semen preparation method (SEP-D Kit) for intra-uterine insemination (IUI): A controlled randomised study to compare the IUI pregnancy outcome between a routine (swim-up) and the SEP-D Kit method.

  19. Evaluation of apricot (Prunus armeniaca L.) seed kernel as a potential feedstock for the production of liquid bio-fuels and activated carbons

    International Nuclear Information System (INIS)

    Fadhil, Abdelrahman B.

    2017-01-01

    Highlights: • Apricot (Prunus armeniaca L.) is presented as a source for biodiesel, bio-oil and activated carbon. • Methylic and ethylic esters of apricot seed kernel oil conformed to ASTM (D6751) standards. • High yield (43.66% w/w) of bio-oil was produced by pyrolysis of de-oiled seed kernel. • High quality of activated carbon was obtained from the biochar. - Abstract: Production of liquid bio-fuels (biodiesel and bio-oil) as well as activated carbon from one non-edible feedstock, apricot (Prunus armeniaca L.) seed kernel was the main objective of the present research work. The oil was extracted from apricot seed kernel with a yield of 49.44% w/w of kernels. Potassium hydroxide-catalyzed transesterification of apricot (Prunus armeniaca L.) seed kernel oil with methanol and ethanol was then applied to produce methylic and ethylic, respectively. Properties of the obtained biodiesels were evaluated and found conformed to ASTM D 6751 limits. The apricot de-oiled seed kernel was pyrolyzed in a semi-batch reactor for bio-oil production. The effect of the pyrolysis temperatures (350, 400, 450, 500, 550 and 600 °C), pyrolysis time (30, 60, 90, 120 and 150 min) and feed particles size (0.25, 0.40, 0.59 and 0.84 mm) on the bio-oil yield was investigated. The maximum production of bio-oil (43.66% w/w) was achieved at a pyrolysis temperature of 450 °C, 60 min pyrolysis time and a feed particles size of 0.25 mm. The bio-oil obtained under the optimal conditions was characterized by the elemental analysis, FTIR spectroscopy and column chromatography. The FTIR analysis of the produced bio-fuel indicated that it composes mainly of alkanes, alkenes, ketones, carboxylic acids and amines. Properties of the resulting bio-oil were analyzed in terms of calorific value, density, flash point, pH, acid value, pour point and refractive index. The properties were close to those of petroleum fractions and comparable to those of other bio-oils published in literature. Referring to

  20. Semen quality in relation to biomarkers of pesticide exposure.

    Science.gov (United States)

    Swan, Shanna H; Kruse, Robin L; Liu, Fan; Barr, Dana B; Drobnis, Erma Z; Redmon, J Bruce; Wang, Christina; Brazil, Charlene; Overstreet, James W

    2003-01-01

    We previously reported reduced sperm concentration and motility in fertile men in a U.S. agrarian area (Columbia, MO) relative to men from U.S. urban centers (Minneapolis, MN; Los Angeles, CA; New York, NY). In the present study we address the hypothesis that pesticides currently used in agriculture in the Midwest contributed to these differences in semen quality. We selected men in whom all semen parameters (concentration, percentage sperm with normal morphology, and percentage motile sperm) were low (cases) and men in whom all semen parameters were within normal limits (controls) within Missouri and Minnesota (sample sizes of 50 and 36, respectively) and measured metabolites of eight current-use pesticides in urine samples provided at the time of semen collection. All pesticide analyses were conducted blind with respect to center and case-control status. Pesticide metabolite levels were elevated in Missouri cases, compared with controls, for the herbicides alachlor and atrazine and for the insecticide diazinon [2-isopropoxy-4-methyl-pyrimidinol (IMPY)]; for Wilcoxon rank test, p = 0.0007, 0.012, and 0.0004 for alachlor, atrazine, and IMPY, respectively. Men from Missouri with high levels of alachlor or IMPY were significantly more likely to be cases than were men with low levels [odds ratios (ORs) = 30.0 and 16.7 for alachlor and IMPY, respectively], as were men with atrazine levels higher than the limit of detection (OR = 11.3). The herbicides 2,4-D (2,4-dichlorophenoxyacetic acid) and metolachlor were also associated with poor semen quality in some analyses, whereas acetochlor levels were lower in cases than in controls (p = 0.04). No significant associations were seen for any pesticides within Minnesota, where levels of agricultural pesticides were low, or for the insect repellent DEET (N,N-diethyl-m-toluamide) or the malathion metabolite malathion dicarboxylic acid. These associations between current-use pesticides and reduced semen quality suggest that

  1. (Prunus armeniaca L.) genotypes

    African Journals Online (AJOL)

    STORAGESEVER

    2009-03-20

    Mar 20, 2009 ... Table 2. Leaf stomatal frequency, stomata size and stomatal conductance of apricot genotypes. Stomatal conductance (mmol m-2 s-1). Apricot genotype. Stomata frequency. (number/mm2). Stomata size. (μm). 2006. 2007. Orange Red. 349.0 a. 71.6 abc. 192 a-d. 176 bc. Palstein. 311.7 ab. 76.6 abc.

  2. Screening for Sexually Transmitted Infection Pathogens in Semen Samples

    Directory of Open Access Journals (Sweden)

    RW Peeling

    2005-01-01

    Full Text Available The transmission of sexually transmitted infection (STI pathogens from an infected donor to the recipient of a semen donation in assisted conception may result not only in acute infection but also in long-term reproductive complications or adverse outcomes of pregnancy, including infection of the offspring. Screening for bacterial STI pathogens, Chlamydia trachomatis and Neisseria gonorrhoeae is strongly recommended because these pathogens can cause serious reproductive complications in the recipients of semen donations and infection in their offspring. Screening for these pathogens should be performed using the most sensitive methods, such as nucleic acid amplified tests. False-negative results due to inhibitory substances in the semen sample should be monitored using amplification controls. Where specimen transport is not a problem and culture facilities are available, N gonorrhoeae can also be detected by culture. Laboratories performing screening should subscribe to proficiency programs and have strict quality controls. Although Trichomonas vaginalis, group B streptococcus and genital mycoplasmas have been associated with adverse outcomes of pregnancy, the frequent finding of these organisms in healthy individuals brings into question the validity of mandatory inclusion of these organisms in the screening panel. Although viral STI pathogens and Treponema pallidum -- the causative agent of syphilis -- may be detected in semen, their presence may be more sensitively detected through antibody testing of the donor. Screening donors for HIV, hepatitis B and syphilis by serology is uniformly recommended in all of the guidelines, but the value of screening either donors or semen samples for cytomegalovirus, herpes simplex viruses and human papilloma viruses is less clear.

  3. Resistance to Plum Pox Virus (PPV) in apricot (Prunus armeniaca L.) is associated with down-regulation of two MATHd genes.

    Science.gov (United States)

    Zuriaga, Elena; Romero, Carlos; Blanca, Jose Miguel; Badenes, Maria Luisa

    2018-01-27

    Plum pox virus (PPV), causing Sharka disease, is one of the main limiting factors for Prunus production worldwide. In apricot (Prunus armeniaca L.) the major PPV resistance locus (PPVres), comprising ~ 196 kb, has been mapped to the upper part of linkage group 1. Within the PPVres, 68 genomic variants linked in coupling to PPV resistance were identified within 23 predicted transcripts according to peach genome annotation. Taking into account the predicted functions inferred from sequence homology, some members of a cluster of meprin and TRAF-C homology domain (MATHd)-containing genes were pointed as PPV resistance candidate genes. Here, we have characterized the global apricot transcriptome response to PPV-D infection identifying six PPVres locus genes (ParP-1 to ParP-6) differentially expressed in resistant/susceptible cultivars. Two of them (ParP-3 and ParP-4), that encode MATHd proteins, appear clearly down-regulated in resistant cultivars, as confirmed by qRT-PCR. Concurrently, variant calling was performed using whole-genome sequencing data of 24 apricot cultivars (10 PPV-resistant and 14 PPV-susceptible) and 2 wild relatives (PPV-susceptible). ParP-3 and ParP-4, named as Prunus armeniaca PPVres MATHd-containing genes (ParPMC), are the only 2 genes having allelic variants linked in coupling to PPV resistance. ParPMC1 has 1 nsSNP, while ParPMC2 has 15 variants, including a 5-bp deletion within the second exon that produces a frameshift mutation. ParPMC1 and ParPMC2 are adjacent and highly homologous (87.5% identity) suggesting they are paralogs originated from a tandem duplication. Cultivars carrying the ParPMC2 resistant (mutated) allele show lack of expression in both ParPMC2 and especially ParPMC1. Accordingly, we hypothesize that ParPMC2 is a pseudogene that mediates down-regulation of its functional paralog ParPMC1 by silencing. As a whole, results strongly support ParPMC1 and/or ParPMC2 as host susceptibility genes required for PPV infection which

  4. Infecciones de transmisión sexual, calidad del semen e infertilidad Sexually transmitted diseases, quality of semen, and infertility

    Directory of Open Access Journals (Sweden)

    Bertha Victoria Rodríguez Pendás

    2008-12-01

    Full Text Available El propósito de este trabajo es insistir en la asociación que existe entre el incremento de las infecciones de transmisión sexual y la infertilidad masculina. Se muestran numerosas investigaciones realizadas en este campo que demuestran el rol de estas infecciones en la etiología de la infertilidad, y se describen algunas de las principales infecciones en el semen que provocan la declinación de la fertilidad masculina y sus consecuencias en la salud reproductiva de los hombres. Con este trabajo de revisión nos proponemos resaltar la necesidad de incluir en el estudio de la infertilidad masculina el control microbiológico del semen, particularmente útil en los servicios de salud reproductiva, donde el riesgo de prevalencia de infecciones asociadas a la infertilidad provoca una reproducción fallida con consecuencias emocionales y sociales en la parejaAim of this paper is to insist on association between the sexually transmitted diseases increase and male infertility. We present most researches performed in this field emphasizing the role of these infections in infertility origin, and we describe also some of main semen infections causing decrease of male fertility, and its consequences on reproductive health of men. Aim of this review paper is to highlight the need of to include in male infertility study, the metabolic control of semen, where risk of infections prevalence associated to infertility provokes a failure reproduction with emotional and social consequences in couple

  5. Long term storage of bee semen – A six month assessment of cryopreserved semen quality using motility as an index

    Science.gov (United States)

    Bee (Apis mellifera Linn.) spermatozoa is known for its ability to remain viable for at the least two years within the spermatheca as attested to by the queens’ continued laying of fertile eggs without remating over two to three (Woyke 1960; Winston 1987). However, the semen collected from the dron...

  6. TEMPORARY STORAGE OF BOVINE SEMEN CRYOPRESERVED IN LIQUID NITROGEN ON DRY ICE AND REFREEZING OF FROZEN-THAWED SEMEN.

    Science.gov (United States)

    Abdussamad, A M; Gauly, M; Holtz, W

    2015-01-01

    Two experiments were conducted. The purpose of Experiment 1 was to investigate whether viability of bovine semen stored in liquid nitrogen (-196°C) will be adversely affected by temporary exposure to dry ice (-79°C). It was convincingly shown that post thaw-motility was not affected, regardless whether semen was thawed immediately or after being returned to liquid nitrogen. Shipping or temporary storage on dry ice, thus, is a viable option. In Experiment 2, refreezing of frozen-thawed semen was attempted. The proportion of motile spermatozoa was reduced by a factor of ten to between 6.0 % and 7.4 %, regardless whether thawing occurred directly after removal from liquid nitrogen or after an interim period on dry ice. When semen was refrozen on dry ice before being returned to liquid nitrogen, motility rates were significantly improved (13.0 % to 17.0 %, P<0.05). In both experiments sperm cells that remained motile displayed vigorous forward movement and normal morphological appearance.

  7. Critical sources of bacterial contamination and adoption of standard sanitary protocol during semen collection and processing in Semen Station

    Directory of Open Access Journals (Sweden)

    Chandrahas Sannat

    2015-05-01

    Full Text Available Aim: The present investigation was conducted to locate the critical sources of bacterial contamination and to evaluate the standard sanitation protocol so as to improve the hygienic conditions during collection, evaluation, and processing of bull semen in the Semen Station. Materials and Methods: The study compared two different hygienic procedures during the collection, evaluation and processing of semen in Central Semen Station, Anjora, Durg. Routinely used materials including artificial vagina (AV inner liner, cone, semen collection tube, buffer, extender/diluter, straws; and the laboratory environment like processing lab, pass box and laminar air flow (LAF cabinet of extender preparation lab, processing lab, sealing filling machine, and bacteriological lab were subjected to bacteriological examination in two phases of study using two different sanitary protocols. Bacterial load in above items/environment was measured using standard plate count method and expressed as colony forming unit (CFU. Results: Bacterial load in a laboratory environment and AV equipments during two different sanitary protocol in present investigation differed highly significantly (p<0.001. Potential sources of bacterial contamination during semen collection and processing included laboratory environment like processing lab, pass box, and LAF cabinets; AV equipments, including AV Liner and cone. Bacterial load was reduced highly significantly (p<0.001 in AV liner (from 2.33±0.67 to 0.50±0.52, cone (from 4.16±1.20 to 1.91±0.55, and extender (from 1.33±0.38 to 0 after application of improved practices of packaging, handling, and sterilization in Phase II of study. Glasswares, buffers, and straws showed nil bacterial contamination in both the phases of study. With slight modification in fumigation protocol (formalin @600 ml/1000 ft3, bacterial load was significantly decreased (p<0.001 up to 0-6 CFU in processing lab (from 6.43±1.34 to 2.86±0.59, pass box (from 12

  8. Semen says: assessing the accuracy of adolescents' self-reported sexual abstinence using a semen Y-chromosome biomarker.

    Science.gov (United States)

    Rosenbaum, Janet E; Zenilman, Jonathan M; Rose, Eve; Wingood, Gina M; DiClemente, Ralph J

    2017-03-01

    Researchers often assess condom use only among participants who report recent sexual behaviour, excluding participants who report no recent vaginal sex or who did not answer questions about their sexual behaviour, but self-reported sexual behaviour may be inaccurate. This study uses a semen Y-chromosome biomarker to assess semen exposure among participants who reported sexual abstinence or did not report their sexual behaviour. This prospective cohort study uses data from 715 sexually active African-American female adolescents in Atlanta, surveyed at baseline, 6 months and 12 months. Participants completed a 40 min interview and were tested for semen Y-chromosome with PCR from a self-administered vaginal swab. We predicted Y-chromosome test results from self-reported sexual behaviour using within-subject panel regression. Among the participants who reported abstinence from vaginal sex in the past 14 days, 9.4% tested positive for semen Y-chromosome. Among item non-respondents, 6.3% tested positive for semen Y-chromosome. Women who reported abstinence and engaged in item non-response regarding their sexual behaviour had respectively 62% and 78% lower odds of testing positive for Y-chromosome (OR 0.38 (0.21 to 0.67), OR 0.22 (0.12 to 0.40)), controlling for smoking, survey wave and non-coital sexual behaviours reported during abstinence. Adolescents who report sexual abstinence under-report semen exposure. Research should validate self-reported sexual behaviour with biomarkers. Adolescents who engage in item non-response regarding vaginal sex test positive for semen Y-chromosome at similar rates, which supports the practice of grouping non-respondents with adolescents reporting abstinence in statistical analysis. NCT00633906. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/.

  9. Air pollution and decreased semen quality: A comparative study of Chongqing urban and rural areas

    International Nuclear Information System (INIS)

    Zhou, Niya; Cui, Zhihong; Yang, Sanming; Han, Xue; Chen, Gangcai; Zhou, Ziyuan; Zhai, Chongzhi; Ma, Mingfu; Li, Lianbing; Cai, Min; Li, Yafei; Ao, Lin; Shu, Weiqun; Liu, Jinyi; Cao, Jia

    2014-01-01

    To investigate the association and effects of air pollution level on male semen quality in urban and rural areas, this study examines the outdoor concentrations of particulate matter (PM 10 ), sulfur dioxide (SO 2 ), nitrous dioxide (NO 2 ) and semen quality outcomes for 1346 volunteers in both urban and rural areas in Chongqing, China. We found the urban area has a higher pollution level than the rural area, contrasted with better semen quality in the rural residents, especially for sperm morphology and computer assistant semen analysis (CASA) motility parameters. A multivariate linear regression analysis demonstrates that concentrations of PM 10 , SO 2 , and NO 2 significantly and negatively are associated with normal sperm morphology percentage (P  10 , SO 2 , and NO 2 in urban ambient air may account for worse semen quality in urban males. - Highlights: • We investigate the distributions of PM 10 , SO 2 and NO 2 in urban and rural areas in Chongqing, China. • We explore the associations of air pollution and male semen quality. • The concentrations of PM 10 , SO 2 , and NO 2 are significantly higher in urban areas. • Median values of some semen quality parameters in rural male were higher than urban male. • PM 10 , SO 2 , and NO 2 were negatively associated with semen quality parameters. - Air pollution is higher in the urban area while there is better semen quality in rural males. Polluted air may thus account for worse semen quality in urban males

  10. Karakteristik Semen Segar dan Kualitas Semen Cair Kuda dalam Pengencer Dimitropoulos yang Disuplementasi dengan Fruktosa, Trehalosa dan Rafinosa

    Directory of Open Access Journals (Sweden)

    Yudi

    2007-12-01

    Full Text Available The objective of the experiment was to study the characteristics of stallion fresh semen and the quality of sperm preserved in Dimitropoulos extender (DV supplemented with different concentration of fructose, trehalose and raffinose. Semen were collected using artificial vagina from three stallions. Semen characteristics and quality were evaluated macro- and microscopically. Prior to extension, semen were centrifugated at 3000 rpm for 20 minutes. The condensed sperm were re-suspended in DV supplemented with different types of carbohydrate to meet the concentration of 200 million spz/ml. All samples were stored at room and chilled temperature, and were evaluated for motility and viability every 3 h and 12 h. The results of the experiments indicated that fresh semen characteristics were fair good; the volume, consistency, motility, live-dead ratio, concentration (106/ml, total spermatozoa (109/ejaculate and abnormality were 29.25±9.33 ml, watery, 7.00±0.12, 67.08±9.08%, 77.89±6.46%, 211.88±21.15, 6.28±2.45 and 27.26±4.64%, respectively. The supplementation of different type and concentration of carbohydrates did not significantly affect the motility and viability. However, the supplementation of 50 mM fructose significantly increased the motility and viability of the sperm compared to the control. In conclusion, carbohydrate supplementation in DV may not maintain the sperm quality, particularly in the medium with the osmolarity higher than 400 mOsm/kg.

  11. Inverse correlation between reactive oxygen species in unwashed semen and sperm motion parameters as measured by a computer-assisted semen analyzer.

    Science.gov (United States)

    Takeshima, Teppei; Yumura, Yasushi; Yasuda, Kengo; Sanjo, Hiroyuki; Kuroda, Shinnosuke; Yamanaka, Hiroyuki; Iwasaki, Akira

    2017-01-01

    This study investigated the correlation between sperm motion parameters obtained by a computer-assisted semen analyzer and levels of reactive oxygen species in unwashed semen. In total, 847 patients, except for azoospermic patients were investigated. At the time of each patient's first consultation, semen parameters were measured using SMAS™ or CellSoft 3000™, and production of reactive oxygen species was measured using a computer-driven LKB Wallac Luminometer 1251 Analyzer. The patients were divided into two groups: reactive oxygen species - positive and negative. The semen parameters within each group were measured using one of the two computer-assisted semen analyzer systems and then compared. Correlations between reactive oxygen species levels and sperm motion parameters in semen from the reactive oxygen species - positive group were also investigated. Reactive oxygen species were detected in semen samples of 282 cases (33.3%). Sperm concentration (P reactive oxygen species - positive group than in the reactive oxygen species - negative group. Among the sperm motion parameters in the reactive oxygen species - positive group, sperm concentration (P reactive oxygen species levels. Therefore, this study demonstrated that excessive reactive oxygen species in semen damage sperm concentration, motility, and other sperm motion parameters.

  12. CONTRIBUTIONS TO THE MORPHOLOGY OF POLLEN FROM SOME VARIETIES OF MALUS DOMESTICA, PYRUS COMMUNIS, PRUNUS DOMESTICA, PRUNUS PERSICA AND PRUNUS ARMENIACA

    Directory of Open Access Journals (Sweden)

    Nicoleta IANOVICI

    2010-01-01

    Full Text Available The objective of this study was to compare Rosaceae genotypes from for their pollen viability and morphology. The pollen of some varieties of Malus domestica, Pyrus communis, Prunus domestica, Prunus persica and Prunus armeniaca was identified. Pollen from mature anthers was collected. Pollen grains of all genera of Rosaceae surveyed occur as radially symmetric isopolar monads. The main aspect studied were the shape of pollen in the polar view (polar perimeter and polar area of pollen. Shape of pollen is triangular-obtuse-convex in polar view. The pollen class is trizonocolpate-obtus-triangular while the sculpturing of exine surface is striate. The number of colpi is three while the spines are absent. Apart from fully developed pollen grains, also much smaller, not completely developed pollen were found in the samples of the examined species. The viability of the pollen was determined on 1% 2,3,5-triphenyl tetrazolium chloride (TTC. Viable, semi-viable and dead pollen numbers and their percentages were determined. This stain test may be used to determine pollen viability in these species to provide only a rough estimate of viability. The results presented here are important for improving our understanding of Rosaceae reproduction biology. Knowledge of reproduction biology, particularly pollen viability and quality, is critical for the newly-developed cultivars.

  13. Effect of Salicylic Acid on Alleviating of Electrolyte Leakage and Flower Organ Damage in Apricot (Prunus armeniaca L. cv. ‘Shahroudi’

    Directory of Open Access Journals (Sweden)

    Morteza ALIREZAIE NOGHONDAR

    2013-02-01

    Full Text Available One of the most important limiting factors in spread of apricot in Iran is late spring frost, which damages flower bud and decrease total yield of crop. It has been found that salicylic acid (SA plays a beneficial role during plant response to chilling and freezing stresses. To evaluate the effects of salicylic acid on alleviating of cold stress, the flower buds (FBs of Prunus armeniaca L. cv. ‘Shahroudi’ were sprayed at pink cluster stage with SA at 4 levels (0, 0.5, 1 and 2 mM and were then exposed to artificial cold stress (4 h at -4°C or without cold stress (+ 25. Experimental attributes including electrolyte leakage of FBs and percentage of damage (PD of pistil, anthers and petals to temperature treatments were determined. The results showed that at -4°C the lowest and highest PD and EL of FBs were observed in application of 0.5 and 0 mM SA, respectively. The highest and lowest PD of flower organ and EL were obtained in application of 0 and 2 mM SA, respectively at +25°C. Based on the results of this experiment, SA alleviates the negative effect of cold stress on electrolyte leakage and flower organ damages in apricot cv. ‘Shahroudi’, depending on the concentrations of SA used.

  14. Semen quality of young men from the general population in Baltic countries

    DEFF Research Database (Denmark)

    Erenpreiss, Juris; Punab, Margus; Zilaitiene, Birute

    2017-01-01

    populations. Assessment of sperm motility is associated with inter-observer variation, and no quality control was undertaken for sperm motility assessment to account for that. Thus, estimates of sperm motility should be interpreted with caution. WIDER IMPLICATIONS OF THE FINDINGS: Analysis of the semen......STUDY QUESTION: What are the parameters of semen quality in Baltic men? SUMMARY ANSWER: Combined parameters of sperm concentration, motility and morphology revealed that 11-15% of men had low semen quality, 37-50% intermediate and 38-52% high semen quality. WHAT IS KNOWN ALREADY: Previous studies...... have revealed regional differences in semen parameters, and semen quality of Baltic men has been suggested to be better than that of other European men. STUDY DESIGN, SIZE, DURATION: This was a cross-sectional study of 1165 men aged 16-29 years from Estonia (N = 573), Latvia (N = 278) and Lithuania (N...

  15. Significance of positive semen culture in relation to male infertility and the assisted reproductive technology process.

    Science.gov (United States)

    Jue, Joshua S; Ramasamy, Ranjith

    2017-10-01

    There are currently no WHO guidelines on the indications for semen culture; however, semen cultures are performed in the evaluation of male infertility and the assisted reproductive technology (ART) process. The relevance and significance of positive semen cultures is widely debated in the literature, with no current consensus on the usefulness of this test in relation to male infertility. We review the pathogenic mechanisms of potentially pathogenic bacteria, general bacteria, urethral flora, and skin flora on sperm parameters. We also present, possible routes of semen contamination, measures to reduce contamination, and the clinical significance of culture contamination. First, it is critical to distinguish round cells in semen as leukocytes from immature germ cells. Second, it is critical to distinguish leukocytospermia from infected semen in order to guide management.

  16. [Compatibility regularity of compound traditional Chinese medicine patents based on association principle and entropy method].

    Science.gov (United States)

    Yin, Xiang-jun; He, Qing-yong

    2015-02-01

    To analyze the compatibility regularity of compound traditional Chinese medicine (TCM) patents for treating dyslipidemia, and provide basis for the clinical development and research of new TCM for treating dyslipidemia. Totally 243 compound traditional Chinese medicine patents for treating dyslipidemia were collected from the national patent database from September 1985 to March 2014 and analyzed by using drug frequency, association rules, complex network and entropy method of Traditional Chinese Medicine Inheritance System (V1.1). The commonest single medicine in the treatment of dyslipidemia is Crataegi Fructus 109 (44.86%). The commonest pair medicine is Crataegi Fructus-Salviae Miltiorrhizae Radix et Rhizoma 53 (21.81%). The commonest corner drug is Crataegi Fructus-Cassiae Semen-Polygoni Multiflori Radix 25 (10.29%). The common prescriptions on basis of association rules are Prunellae Spica-->Salviae Miltiorrhizae Radix et Rhizoma (0.833), Rhei Radix et Rhizoma, Alismatis Rhizoma-->Polygoni Multiflori Radix (1.00), Salviae Miltiorrhizae Radix et Rhizoma, Cassiae Semen, Alismatis Rhizoma-->Polygoni Multiflori Radix (0.929). The core drugs based on complex networks are Salviae Miltiorrhizae Radix et Rhizoma and Crataegi Fructus. The new prescriptions extracted by entropy method are Atractylodis Macrocephalae Rhizoma-Glycyrrhizae Radix et Rhizoma-Platycladi Semen-Stephaniae Tetrandrae Radix; Citri Reticulatae Pericarpium-Poria-Coicis Semen-Pinelliae Rhizoma. This study shows the regularity in the compatibility of compound TCM patents treating dyslipidemia, suggesting that future studies on new traditional Chinese medicines treating dyslipidemia should focus on the following six aspects: (1) Single medicine should be preferred: e. g. Crataegi Fructus; (2) Pair medicines should be preferred: e. g. Crataegi Fructus-Salviae Miltiorrhizae Radix et Rhizoma; (3) Corner drugs should be preferred: e. g. Crataegi Fructus, Cassiae Semen, Polygoni Multiflori Radix; (4) The

  17. Clinical and biochemical correlates of successful semen collection for cryopreservation from 12-18-year-old patients: a single-center study of 86 adolescents

    DEFF Research Database (Denmark)

    Hagenäs, Isabella; Jørgensen, Niels; Rechnitzer, Catherine

    2010-01-01

    Cryopreservation of semen should be offered to adults before gonadotoxic treatment. However, the experience with semen collection in adolescents is still limited. The objective of this study was to evaluate potential correlates of successful semen sampling in adolescents.......Cryopreservation of semen should be offered to adults before gonadotoxic treatment. However, the experience with semen collection in adolescents is still limited. The objective of this study was to evaluate potential correlates of successful semen sampling in adolescents....

  18. Good semen quality and life expectancy: A cohort study of 43,277 men

    DEFF Research Database (Denmark)

    Jensen, Tina Kold; Jacobsen, Rune; Christensen, Kaare

    2009-01-01

    and morphologically normal spermatozoa and semen volume increased, mortality decreased in a dose-response manner (P(trend) good semen quality was due to a decrease in a wide range of diseases and was found among men both with and without children; therefore......, the decrease in mortality could not be attributed solely to lifestyle and/or social factors. Semen quality may therefore be a fundamental biomarker of overall male health....

  19. Pembuatan Dan Kararkterisasi Kertas Yang Dibuat Dari Kantong Semen Bekas Dengan Pulp Batang Kelapa Sawit

    OpenAIRE

    Ederiana Br. Sidebang

    2008-01-01

    Penelitian tentang pembuatan dan karakterisasi kertas yang dibuat dari campuran pulp batang kelapa sawit dan pulp kantong semen bekas telah dilakukan. Penelitian yang dilaksanakan dengan metode proses soda, bertujuan untuk memperoleh alternatif bahan baku kertas campuran, dari pulp batang kelapa sawit dan kantong semen bekas. Untuk campuran 0% pulp batang kelapa sawit dan 100% pulpkantong semen bekas,diperoleh contoh kertas yang mempunyai gramatur rata rata 103,63 gr/m, kerapatan massa rata r...

  20. Pembuatan Dan Karakterisasi Kertas Pembungkus Yang Dibuat Dari Kantong Semen Bekas Dengan Pulp Jerami

    OpenAIRE

    Dormian Saragi

    2008-01-01

    Penelitian tentang pembuatan dan karakterisasi kertas yang dibuat dari campuran pulp jerami dan pulp kantong semen bekas telah dilakukan. Penelitian yang dilaksanakan dengan metode proses soda, bertujuan untuk memperoleh alternatif bahan baku kertas campuran, dari pulp jerami dan kantong semen bekas. untuk campuran 0 % pulp jerami dan 100 % pulp kantong semen bekas, diperoleh contoh kertas yang mempunyai gramatur rata-rata 108,13 gr/m2, kerapatan massa rata-rata 720,89 kg/m3, kuat tarik rata-...

  1. Semen Quality as a Predictor of Subsequent Morbidity

    DEFF Research Database (Denmark)

    Latif, Tabassam; Jensen, Tina Kold; Mehlsen, Jesper

    2017-01-01

    in the Danish National Patient Registry until first hospitalization, death, or the end of the study.We classified patients according to hospitalizations and the presence of cardiovascular disease, diabetes, testicular cancer, or prostate cancer. We found a clear association between sperm concentration below 15...... with a low total sperm count and low motility. Men with a sperm concentration of 195-200million/mL were, on average, hospitalized for the first time 7 years later than were men with a sperm concentration of 0-5million/mL. Semen quality was associated with long-term morbidity, and a significantly higher risk...... of hospitalization was found, in particular for cardiovascular diseases and diabetes mellitus. Our study supports the suggestion that semen quality is a strong biomarker of general health....

  2. Semen collection and evaluation of captive coatis (Nasua nasua

    Directory of Open Access Journals (Sweden)

    R.C.R. Paz

    2012-04-01

    Full Text Available Semen samples (n=105 were collected through eletroejaculation from six adult male coatis (Nasua nasua between January 2007 and December 2008 at Universidade Federal de Mato Grosso Zoo, Cuiabá, Brazil. Mean values were: volume (mL; concentration (sperm/mL; total motility (%; progressive sperm motility (scale, 0-5; live spermatozoa (%; acrossome integrity (%; primary defects (%; and secondary defects (%. There was high correlation between total motility and live sperm; total motility and progressive sperm motility; total motility and acrossome integrity; live sperm and progressive motility; live sperm and acrossome integrity and volume and concentration. The method for semen collection was considered safe and efficient. It can be used for the evaluation of breeding potential of coati in captivity and for the establishment of new assisted reproductive technology (ART for threatened neotropical carnivores species.

  3. Association of Sleep Disturbances With Reduced Semen Quality

    DEFF Research Database (Denmark)

    Jensen, Tina Kold; Andersson, Anna-Maria; Skakkebæk, Niels Erik

    2013-01-01

    Several studies have found an association between sleep duration and morbidity and mortality, but no previous studies have examined the association between sleep disturbances and semen quality. We conducted a cross-sectional study among 953 young Danish men from the general population who were...... recruited in Copenhagen at the time of determination of fitness for military service between January 2008 and June 2011. All of the men delivered a semen sample, had a blood sample drawn, underwent a physical examination, and answered a questionnaire including information about sleep disturbances. Sleep...... disturbances were assessed on the basis of a modified 4-item version of the Karolinska Sleep Questionnaire, which includes questions on sleep patterns during the past 4 weeks. Sleep disturbances showed an inverse U-shaped association with sperm concentration, total sperm count, percent motile and percent...

  4. Preliminary results on the collection and dilution of ostrich semen

    African Journals Online (AJOL)

    Maud Bonato

    spermatozoa 3.68 ± 0.17 x 109 /mL and the number of spermatozoa 4.92 ± 0.64 x 109 /ejaculate. Furthermore, the live normal, live abnormal and dead spermatozoa in the neat semen were 61.2 ± 4.5%, 21.2. ± 2.7% and 17.7 ± 4.3% respectively. The ejaculate volume and the number of dead spermatozoa were not affected ...

  5. Effect of L-carnitine supplementation on drake semen quality

    African Journals Online (AJOL)

    sp3

    2014-01-30

    Jan 30, 2014 ... (150 mg L-carnitine/kg diet) recorded the best results in relation to all semen quality traits included in this study. Dietary ..... L-carnitine levels in the seminal plasma of fertile and infertile men: correlation with sperm quality. Int. J. Fert. Womens Med. 45, 236-240. Neuman, S.L., Lint, T.L. & Hester, P.Y., 2002.

  6. Removal of bacteria from stallion semen by colloid centrifugation.

    Science.gov (United States)

    Morrell, J M; Klein, C; Lundeheim, N; Erol, E; Troedsson, M H T

    2014-02-01

    Bacteria (environmental contaminants and occasionally potential pathogens) are found in most stallion ejaculates and may negatively affect sperm quality during storage. Since the use of antibiotics can lead to the development of resistance, an alternative means of microbial control is desirable. The removal of bacteria from stallion semen using Single Layer Centrifugation through Androcoll-E was investigated. Known doses of cultured bacteria were added to freshly collected ejaculates (15mL aliquots) before processing by Single Layer Centrifugation. The resulting sperm pellets and controls (not processed by Single Layer Centrifugation) were cultured and the bacteria identified. In experiment 1, doses of E. coli from 2×10(2) to 2×10(7) colony forming units were added to aliquots of semen. In experiment 2, Taylorella equigenitalis or a mix of E. coli, Klebsiella pneumoniae and Streptococcus equi subsp. zooepidemicus (approximately 7×10(6), 5×10(6), and 6×10(6)cfu, respectively) were added to 15mL aliquots of semen. In experiment 1, more than 90% of the bacteria were removed where loading doses were >×10(4)cfu/mL. In experiment 2, varying proportions of different bacteria were removed, ranging from 68% for naturally occurring Corynebacterium spp. to >97% for added cultured E. coli. Thus, Single Layer Centrifugation can separate spermatozoa from many, but not all bacteria in stallion ejaculates and could be a useful alternative to adding antibiotics to semen extenders to control bacterial contamination. However, further research is needed to determine the effect of small numbers of bacteria on sperm quality. Copyright © 2014 Elsevier B.V. All rights reserved.

  7. Influence of different centrifugation protocols on equine semen preservation.

    Science.gov (United States)

    Hoogewijs, Maarten; Rijsselaere, Tom; De Vliegher, Sarne; Vanhaesebrouck, Emilie; De Schauwer, Catharina; Govaere, Jan; Thys, Mirjan; Hoflack, Geert; Van Soom, Ann; de Kruif, Aart

    2010-07-01

    Three experiments were conducted to evaluate the impact of centrifugation on cooled and frozen preservation of equine semen. A standard centrifugation protocol (600 x g for 10 min=CP1) was compared to four protocols with increasing g-force and decreased time period (600 x g, 1200 x g, 1800 x g and 2400 x g for 5 min for CP2, 3, 4, and 5, respectively) and to an uncentrifuged negative control. In experiment 1, the influence of the different CPs on sperm loss was evaluated by calculating the total number of sperm cells in 90% of the supernatant. Moreover, the effect on semen quality following centrifugation was assessed by monitoring several sperm parameters (membrane integrity using SYBR14-PI, acrosomal status using PSA-FITC, percentage total motility (TM), percentage progressive motility (PM) and beat cross frequency (BCF) obtained with computer assisted sperm analysis (CASA)) immediately after centrifugation and daily during chilled storage for 3 d. The use of CP1 resulted in a sperm loss of 22%. Increasing the centrifugation force to 1800 x g and 2400 x g for 5 min led to significantly lower sperm losses (7.4% and 2.1%, respectively; Pcentrifugation of semen resulted in a better sperm quality after chilled storage. There were minimal differences between the CPs although total motility was lower for CP2 than for the other treatments (Pcentrifuged samples were cryopreserved using a standard freezing protocol and analyzed immediately upon thawing. Samples centrifuged according to CP2 resulted in a higher BCF (Pcentrifugation can be substantially reduced by increasing the g-force up to 1800 x g or 2400 x g for a shorter period of time (5 min) compared to the standard protocol without apparent changes in semen quality, resulting in a considerable increase in the number of insemination doses per ejaculate.

  8. [Effects of straw handling during storage on semen quality in the bull].

    Science.gov (United States)

    Janett, F; Schilter, E; Weber, F; Witschi, U; Thun, R

    2008-12-01

    The aim of the present study was to investigate the influence of semen handling by different inseminators on quality of frozen-thawed bovine semen. For this study a total of 10 ejaculates were collected from 7 bulls and semen stored frozen for 7 months in vessels of 10 inseminators each, in three different regions of Switzerland (A, B and C). After storage motility and acrosome integrity (live spermatozoa with intact acrosomes) were measured in frozen-thawed semen by computer-assisted sperm analysis (CASA) and flowcytometry, respectively. In addition, the 56 days non-return rate (NRR) was determined for each inseminator and the relation between NRR of single inseminators and semen quality examined. Results demonstrate that motility as well as acrosome integrity of stored frozen semen were significantly influenced by the specific delivery system used in the different regions and by the ejaculate. After storage of straws in region B semen motility was significantly (P < 0.05) lower than in region C. In region B the percentage live and acrosome intact spermatozoa as well as the NRR of the inseminators were significantly (P < 0.05) lower than in region A. Motility and acrosome integrity of semen stored by single inseminators were significantly correlated with the inseminator's NRR. We conclude that the quality of frozen semen clearly varied between inseminators of the three regions with an impact on NRR.

  9. Pengaruh Berbagai Konsentrasi Dimethylsulfoxide terhadap Kualitas Semen Beku Ayam Hutan Hijau Post Thawing

    Directory of Open Access Journals (Sweden)

    Wayan Bebas

    2015-06-01

    Full Text Available The process of freezing and thawing on semen can lead to physical stress, often called cold shock, and couses the structural and biochemical damage that affecting cell function and ultimately lead to the death of the cell The aim of this study was to know the effect of the addition of various concentrations of dimethylsulfoxide (DMSO as the intracellular cryoprotectant in phosphate yolk diluent on the post thowing quality of the green jungle fowl semen. The study used eight green jungle fowl semens which were collected with massage techniques. Semen was evaluated macroscopically and microscopically. Good quality semen was diluted with phosphate yolk which was added four different concentration of DMSO, namely 4%, 6%, 8%, and 10%. Semen was then filled and sealed in a mini straw (0.25 mL with the concentration of 150.106 cells, and equilibrated at 4oC for 4 hours. The semen freezing was processed using conventional method. Evaluation was performed on post thawing semen. The evaluation of semen quality included the progressive motility and plasma membrane intact. Data were analyzed by analysis of variance. If there were any significant differences, the data were futher analyzed by Duncan test. The results showed that addition of DMSO concentration of 6% has resulted the progressive motility and intact plasma membrane higher significantly (P <0.05 than those of the addition of DMSO concentration 4%, 8%, and 10%.

  10. Prospective surveillance of semen quality in the workplace

    Energy Technology Data Exchange (ETDEWEB)

    Schenker, M.B.; Samuels, S.J.; Perkins, C.; Lewis, E.L.; Katz, D.F.; Overstreet, J.W.

    1988-04-01

    We performed a prospective surveillance of semen quality among workers in the plant where 1,2-dibromo-3-chloropropane was first recognized as an occupational cause of impaired semen quality and of infertility. All male employees of the Agricultural Chemical Division were required to participate. Ninety-seven workers (92% participation) provided 258 semen samples over the 4 years of the program. Most samples were analyzed at the plant with a mini-laboratory designed for the study. Motility and shape measures were made objectively. Sixty-six subjects (68%) were non-azoospermic. Generalized multiple regression showed no significant predictors for any response, with the exception of the motility measures, which were reduced with longer times between ejaculation and assay. Between- and within-person standard deviations and correlations were calculated. Comparison of this population with fertile artificial insemination donors (16 men, 498 ejaculates) revealed generally higher ejaculate-to-ejaculate standard deviations in the worker samples. This is probably due to less well controlled conditions of sperm collection in the workplace setting. For cross-sectional studies, one ejaculate per worker is recommended as sufficient; for estimating an individual worker's mean, even three ejaculates may not provide enough precision.

  11. Regional differences in semen qualities in the Baltic region.

    Science.gov (United States)

    Punab, Margus; Zilaitiene, Birute; Jørgensen, Niels; Horte, Antero; Matulevicius, Valentinas; Peetsalu, Ants; Skakkebaek, Niels E

    2002-08-01

    Recent prospective studies of male reproductive health have shown differences between several European countries. Our objective was to evaluate the current situation in the two Baltic States Estonia and Lithuania. In 1997-99 we investigated semen parameters, levels of reproductive hormones and general health factors of 196 men from the general population in Lithuania; from Estonia, 79 men from the general population and 118 soldiers were investigated. Adjusted for interlaboratory differences and abstinence period, sperm concentration of Lithuanian and Estonian men from the general populations were shown to be 55 and 67 million/mL, respectively. The Estonian soldiers had the highest sperm concentration, 82 million/mL. The frequencies of morphologically normal spermatozoa were 6.2, 7.7 and 9.6%, respectively. In contrast to the semen qualities, highest Inhibin B levels were detected in the Lithuanians (233 pg/mL) followed by Estonian men from the general population (220 pg/mL) and Estonian soldiers (185 pg/mL). The soldiers had also the lowest level of testosterone and oestradiol. The sperm counts of the Estonian and Lithuanian men investigated here are higher than recently shown for Norwegian, Danish, Estonian and Finnish men. Comparisons should be cautiously drawn as the groups are not completely comparable. Still, even within the Baltic region, geographically close and sharing common recent social history, differences in semen quality and levels of reproductive hormones are apparent.

  12. Sperm banking for male cancer patients: social and semen profiles

    Directory of Open Access Journals (Sweden)

    Tatiana C.S. Bonetti

    2009-04-01

    Full Text Available PURPOSE: Report the characteristics of cryopreserved semen from a cohort of male cancer patients, attitudes towards cryopreservation and outcomes of semen samples based on a 12-year cryopreservation program. MATERIAL AND METHODS: Data from 98 male cancer patients whose sperm samples were banked were evaluated. Demographic parameters, semen characteristics, destination of sperm banked samples and questionnaires answered by the patients regarding cryopreservation time were evaluated. RESULTS: The cancer diagnoses were testicle (56.1%, prostate (15.3%, Hodgkin’s lymphomas (9.2%, non-Hodgkin’s lymphomas (7.1%, leukemia (3.1% and other malignancies (9.2%. The patients with testicular cancer presented lower sperm concentration (p < 0.001; however, there were no differences with the percentage of normozoospermic patients among cancer type groups (p = 0.185. A shorter time between cancer diagnosis and sperm banking was observed for testicular and prostate cancer patients (p < 0.001. Most of the patients (89.5% favored sperm banking as a fertility preservation method. CONCLUSIONS: Although less than 20% of banked sperm samples were disposed of, the majority of patients related sperm banking with safe for fertility preservation. Our results show that all male cancer patients of reproductive age facing cancer treatment could be offered sperm banking.

  13. Decreases in Human Semen Quality with Age Among Healthy Men

    Energy Technology Data Exchange (ETDEWEB)

    Eskenazi, B.; Wyrobek, A.J.; Kidd, S.A.; Moore, L.; Young, S.S.; Moore, D.

    2001-12-01

    The objective of this report is to characterize the associations between age and semen quality among healthy active men after controlling for identified covariates. Ninety-seven healthy, nonsmoking men between 22 and 80 years without known fertility problems who worked for or retired from a large research laboratory. There was a gradual decrease in all semen parameters from 22-80 years of age. After adjusting for covariates, volume decreased 0.03 ml per year (p = 0.001); sperm concentration decreased 2.5% per year (p = 0.005); total count decreased 3.6% per year of age (p < 0.001); motility decreased 0.7% per year (P < 0.001); progressive motility decreased 3.1% per year (p < 0.001); and total progressively motile sperm decreased 4.8% per year (p < 0.001). In a group of healthy active men, semen volume, sperm concentration, total sperm count, and sperm motility decrease continuously between 22-80 years of age, with no evidence of a threshold.

  14. Glyph-Based Video Visualization for Semen Analysis

    KAUST Repository

    Duffy, Brian

    2015-08-01

    © 2013 IEEE. The existing efforts in computer assisted semen analysis have been focused on high speed imaging and automated image analysis of sperm motility. This results in a large amount of data, and it is extremely challenging for both clinical scientists and researchers to interpret, compare and correlate the multidimensional and time-varying measurements captured from video data. In this work, we use glyphs to encode a collection of numerical measurements taken at a regular interval and to summarize spatio-temporal motion characteristics using static visual representations. The design of the glyphs addresses the needs for (a) encoding some 20 variables using separable visual channels, (b) supporting scientific observation of the interrelationships between different measurements and comparison between different sperm cells and their flagella, and (c) facilitating the learning of the encoding scheme by making use of appropriate visual abstractions and metaphors. As a case study, we focus this work on video visualization for computer-aided semen analysis, which has a broad impact on both biological sciences and medical healthcare. We demonstrate that glyph-based visualization can serve as a means of external memorization of video data as well as an overview of a large set of spatiotemporal measurements. It enables domain scientists to make scientific observation in a cost-effective manner by reducing the burden of viewing videos repeatedly, while providing them with a new visual representation for conveying semen statistics.

  15. Impact of silymarin enriched semen extender on bull sperm preservability

    Directory of Open Access Journals (Sweden)

    El-Sheshtawy RI

    2017-02-01

    Full Text Available Objective: To explore the effect of silymarin on bull spermatozoa during cooling and cryopreservation. Methods: Pooled bull semen were diluted by Tris-Citrate-Fructose egg yolk diluents, purified silymarin powder (obtained from the milk thistle silybum marianum, purchased from Unipharma, Al Obour city, Egypt, was soaked in Tris-citric acid-fructose diluent for 48 h at 10 ℃ making a stock solution (70 mg/mL, from this stock solution we obtained concentrations of 0.18 mg/mL, 0.36 mg/mL, 0.54 mg/mL, 0.72 mg/mL, 0.90 mg/mL in addition to the control (0.00 mg/mL reaching a final volume of 5 mL in each tube. Egg yolk was added to each tube to obtain silymarin enriched semen extender (SEE with 20% egg yolk, cooled slowly up to 5 曟 and equilibrated for 4 h. Semen was packed into 0.25 mL polyvinyl French straws (IMV, France. After equilibration periods, the straws were placed horizontally on a rack and frozen in a vapor 4 cm above liquid nitrogen for 10 min and were then dipped in liquid nitrogen. Extended semen was subjected to evaluation (motility, alive%, abnormality%, intact sperm membrane (HOST% and conception rate in both chilled and frozen semen. Results: Table 1 revealed that Sperm motility of the concentrations 2, 3 and 4 after 8 d of chilling were significantly (P<0.02 higher than control. Sperm motility of the concentration 2 (45.00%±2.89% after 9 d of chilling was higher than control and the other concentrations. Addition of SEE in concentration 1 and 2 gave post thawing sperm motility as high as the control (47.50±2.81 and 45.00±2.58, respectively while other concentration have lower effects on motility as compared to the control. Addition of silymarin improved post thawing alive% and was significantly higher (P<0.000 1 than the control. SEE decreased significantly (P<0.000 1 the % of post thawing abnormal sperm in concentration 3 and 4 (11.83±0.65 and 16.00±0.58, respectively. SEE improved significantly (P<0.018 the % of post

  16. Simultaneous qualification and quantification of baccharane glycosides in Impatientis Semen by HPLC-ESI-MSD and HPLC-ELSD.

    Science.gov (United States)

    Li, Hui-Jun; Yu, Jun-Jie; Li, Ping

    2011-03-25

    This study presents a high performance liquid chromatography (HPLC) with electrospray ionization mass spectrometric detection (ESI-MSD) and evaporative light scattering detection (ELSD) method for the simultaneous qualification and quantification of eight major baccharane glycosides, namely hosenlosides A, B, C, F, G, K, L, and M in Impatientis Semen, a Chinese herbal medicine derived from the seeds of Impatiens balsamina L. In order to achieve optimum performance, several extraction parameters (including extraction solvent, extraction mode, extraction time) were optimized. The baccharane glycosides were separated on a Shim-pack CLC-ODS column with gradient elution of water and methanol. Temperature for the ELSD drift tube was set at 98°C and the nitrogen flow rate was 2.7l/min. The unambiguous identities of the analytes were realized by comparing retention times and mass data with those of reference compounds. The developed method was fully validated in terms of linearity, sensitivity, precision, repeatability, recovery as well as robustness, and subsequently applied to evaluate the quality of 14 batches of Impatientis Semen commercial samples from different collections. Copyright © 2010 Elsevier B.V. All rights reserved.

  17. Liposome encapsulated soy lecithin and cholesterol can efficiently replace chicken egg yolk in human semen cryopreservation medium.

    Science.gov (United States)

    Mutalik, Srinivas; Salian, Sujith Raj; Avadhani, Kiran; Menon, Jyothsna; Joshi, Haritima; Hegde, Aswathi Raju; Kumar, Pratap; Kalthur, Guruprasad; Adiga, Satish Kumar

    2014-06-01

    Cryopreservation of spermatozoa plays a significant role in reproductive medicine and fertility preservation. Chicken egg yolk is used as an extender in cryopreservation of human spermatozoa using glycerol egg yolk citrate (GEYC) buffered medium. Even though 50% survival of spermatozoa is generally achieved with this method, the risk of high levels of endotoxins and transmission pathogens from chicken egg yolk is a matter of concern. In the present study we attempted to establish a chemically defined cryopreservation medium which can replace the chicken egg yolk without affecting sperm survival. Ejaculates from 28 men were cryopreserved with GEYC based freezing medium or liposome encapsulated soy lecithin-cholesterol based freezing medium (LFM). The semen samples were subjected to rapid thawing after 14 days of storage in liquid nitrogen. Post-thaw analysis indicated significantly higher post-thaw motility and sperm survival in spermatozoa cryopreserved with LFM compared to conventional GEYC freezing medium. The soy lecithin and cholesterol at the ratio of 80:20 with sucrose showed the highest percentage of post-thaw motility and survival compared to the other compositions. In conclusion, chemically defined cryopreservation medium with liposome encapsulated soy lecithin and cholesterol can effectively replace the chicken egg yolk from human semen cryopreservation medium without compromising post-thaw outcome.

  18. Carotenoids, Tocopherols and Antioxidant Activity of Lipophilic Extracts from Sea Buckthorn Berries (Hippophae rhamnoides, Apricot Pulp and Apricot Kernel (Prunus armeniaca

    Directory of Open Access Journals (Sweden)

    Elena Andreea Pop

    2015-11-01

    Full Text Available A healthy human diet requires the daily consumption of fruits and vegetables rich in bioactive compounds. Sea buckthorn berries (Hippophae rhamnoides L. and apricot fruits (Prunus armeniaca L. are cultivated and appreciated in Romania both as fresh fruits and as derived products. Characterized by a complex chemical composition, sea buckthorn is rich in unsaturated lipids, carotenoids and tocopherols. Except for β-carotene content, less is known about other lipophilic compounds in apricot fruits. The aim of this paper was to separate and quantify the individual carotenoids, tocopherols and tocotrienols in sea buckthorn, apricot pulp and kernels and also to determine the antioxidant activity of the lipophilic extracts using the TEAC  method.  Chemical characterization of lipophilic extract was performed by HPLC with PDA and fluorescence detection. The total carotenoid content was 17.19±1.4 mg/100g F.W. in sea buckthorn; 3.51±0.25 mg/100g F.W. in apricot fruits and 0.58±0.04 mg/100 g F.W. in apricot kernels. The major carotenoids in sea buckthorn were β-carotene, zeaxanthin and β-cryptoxanthin esters. Apricots fruits are rich in β-carotene and its geometric isomers while in kernels we could properly identified only lycopene. The α-tocopherol concentration was higher in sea buckthorn (46 mg/kg than in apricot fruits (1.09 mg/kg while apricot kernel contain large amounts of γ-tocopherol (111 mg/kg. Sea buckthorn fruits showed the highest antioxidant capacity, correlated with a high content of both tocopherols and carotenoids.

  19. Physically Active Men Show Better Semen Parameters than Their Sedentary Counterparts.

    Science.gov (United States)

    Lalinde-Acevedo, Paula C; Mayorga-Torres, B Jose Manuel; Agarwal, Ashok; du Plessis, Stefan S; Ahmad, Gulfam; Cadavid, Ángela P; Cardona Maya, Walter D

    2017-10-01

    The quality of semen depends upon several factors such as environment, life style, physical activity, age, and occupation. The aim of this study was to analyze and compare the conventional and functional semen parameters in men practicing vigorous physical activity to those of sedentary men. In this descriptive cross-sectional study, semen samples of 17 physically active men and 15 sedentary men were collected for analysis. Semen analysis was performed according to the World Health Organization (WHO) guidelines, while functional parameters were evaluated by flow cytometry. Results showed that several semen parameters (semen volume, viability, progressive motility, total motility, normal morphology, and moribund cells) were superior in the physically active group in comparison with the sedentary group. Semen parameters such as viability, progressive motility and total motility, as well as the percentage of moribund spermatozoa were significantly different between both groups. However, sperm DNA damage, lipid peroxidation and mitochondrial potential were not significantly different among the groups. Nevertheless, the physical activity shows better semen parameters than sedentary group. Taken together, our results demonstrate that regular physical activity has beneficial impact in sperm fertility parameters and such a life style can enhance the fertility status of men. Copyright© by Royan Institute. All rights reserved.

  20. Serum concentration of anti-Müllerian hormone is not associated with semen quality

    DEFF Research Database (Denmark)

    Aksglaede, L; Olesen, I A; Carlsen, E

    2018-01-01

    Impaired semen quality is frequent in Western countries and is the main reason or contributing reason in up to 50% of cases of couple infertility. Male factor infertility is mainly determined by examination of semen samples according to the World Health Organization's 2010 guidelines. AMH has both...

  1. Reproducibility and reliability of repeated semen analyses in male partners of subfertile couples

    NARCIS (Netherlands)

    Leushuis, Esther; van der Steeg, Jan Willem; Steures, Pieternel; Repping, Sjoerd; Bossuyt, Patrick M. M.; Blankenstein, Marinus A.; Mol, Ben Willem J.; van der Veen, Fulco; Hompes, Peter G. A.

    2010-01-01

    Objective: To determine the precise degree of variability that is represented by the reproducibility and reliability of semen analysis. The general assumption is that semen analyses need to be repeated because of a high degree of within-individual variability. However, the precise degree of

  2. Computer-assisted semen analysis parameters as predictors for fertility of men from the general population

    DEFF Research Database (Denmark)

    Larsen, L; Scheike, Thomas Harder; Jensen, Tina Kold

    2000-01-01

    The predictive value of sperm motility parameters obtained by computer-assisted semen analysis (CASA) was evaluated for the fertility of men from general population. In a prospective study with couples stopping use of contraception in order to try to conceive, CASA was performed on semen samples...

  3. Caffeine Intake and Semen Quality in a Population of 2,554 Young Danish Men

    DEFF Research Database (Denmark)

    Ramlau-Hansen, Cecilia; Thulstrup, Ane Marie; Bonde, Jens Peter

    2010-01-01

    The authors examined the association between semen quality and caffeine intake among 2,554 young Danish men recruited when they were examined to determine their fitness for military service in 2001-2005. The men delivered a semen sample and answered a questionnaire including information about...

  4. PCR assessment of Chlamydia trachomatis infection of semen specimens processed for artificial insemination

    NARCIS (Netherlands)

    Pannekoek, Y.; Westenberg, S. M.; de Vries, J.; Repping, S.; Spanjaard, L.; Eijk, P. P.; van der Ende, A.; Dankert, J.

    2000-01-01

    In order to ascertain the microbiological quality of stored semen specimens processed for artificial insemination by a donor (AID), we developed a PCR assay targeting the chlamydial plasmid to detect Chlamydia trachomatis in semen. The lower limit of detection of this assay corresponded to 2.5 to 5

  5. 19 Beef cattle pregnancy rates following insemination with aged frozen angus semen

    Science.gov (United States)

    Artificial insemination has proven to be a valuable asset to the cattle industry. It is assumed that once good quality semen is frozen in liquid nitrogen it should remain viable indefinitely; however, semen viability has not been systematically evaluated after being stored for several decades. In th...

  6. ANALISIS PENGARUH STRATEGI PEMASARAN DALAM PENINGKATAN PANGSA PASAR PADA PT. SEMEN TONASA

    OpenAIRE

    ARIANSYAH, IRWIN

    2014-01-01

    2014 ABSTRAK ANALISIS PENGARUH STRATEGI PEMASARAN DALAM PENINGKATAN PANGSA PASAR PADA PT. SEMEN TONASA Analysis Affect of Marketing Strategy in Increasing of Market Share on Semen Tonasa Ltd. Irwin Ariansyah H. Cepi Pahlevi Wardhani Hakim Penelitian ini bertujuan untuk mengkaji dan menganalisis pengaruh strategi pemasaran yang terdiri atas segmentasi, target dan posisi pasar terhadap peningkatan pangsa pas...

  7. Application of liquid semen technology under the seasonal dairy production system in New Zealand.

    Science.gov (United States)

    Yang, D H; Standley, N T; Xu, Z Z

    2018-01-16

    Systems for preserving semen in liquid form for artificial insemination were developed before cryopreserved semen became widely available in the 1960s. Advantages of liquid semen include reduced number of sperm per dose, reduced storage and transportation costs, increased insemination speed and safety in the field. A liquid semen dose requires one tenth the sperm number in a frozen semen dose to achieve equivalent fertility (24 day non-return rate: 67.6% for liquid versus 67.8% for frozen). The main disadvantage of liquid semen is its relatively short shelf life, thus limiting its application mainly to countries, like New Zealand and Ireland, with predominantly seasonal dairy production systems. Nevertheless, successful application of liquid semen technology can improve the rate of genetic gain by increasing the utilization of elite sires. This brief review covers the principles of liquid semen preservation and describes why and how this technology is implemented by Livestock Improvement Corporation in New Zealand. Copyright © 2018 Elsevier B.V. All rights reserved.

  8. Effect of HIV-1 and Antiretroviral Therapy on Semen Quality | Ntim ...

    African Journals Online (AJOL)

    The results showed that all the critical semen parameters (sperm motility, sperm concentration, total sperm count, sperm mor-phology, sperm vitality, semen volume and pH) were impaired in the HIV-1 infected groups. How-ever, sperm motility improved with longer duration of HAART treatment than shorter duration.

  9. Exposure to perfluorinated compounds and human semen quality in Arctic and European populations

    DEFF Research Database (Denmark)

    Toft, G; Jönsson, B A G; Lindh, C H

    2012-01-01

    Perfluorinated compounds (PFCs) have been suspected to adversely affect human reproductive health. The aim of this study was to investigate the associations between PFC exposure and male semen quality.......Perfluorinated compounds (PFCs) have been suspected to adversely affect human reproductive health. The aim of this study was to investigate the associations between PFC exposure and male semen quality....

  10. Semen profiles of young men involved as bicycle taxi cyclists in ...

    African Journals Online (AJOL)

    Aim The purpose of this study was to evaluate the semen profiles of bicycle taxi cyclists and healthy controls in Mangochi district, Malawi. Methods Semen samples were collected from young bicycle taxi cyclists after two to three days of sexual abstinence. A control group, comprising young men who were not bicycle taxi ...

  11. Ebola Virus RNA in Semen from an HIV-Positive Survivor of Ebola.

    Science.gov (United States)

    Purpura, Lawrence J; Rogers, Emerson; Baller, April; White, Stephen; Soka, Moses; Choi, Mary J; Mahmoud, Nuha; Wasunna, Christine; Massaquoi, Moses; Kollie, Jomah; Dweh, Straker; Bemah, Philip; Ladele, Victor; Kpaka, Jonathan; Jawara, Mary; Mugisha, Margaret; Subah, Onyekachi; Faikai, Mylene; Bailey, Jeff A; Rollin, Pierre; Marston, Barbara; Nyenswah, Tolbert; Gasasira, Alex; Knust, Barbara; Nichol, Stuart; Williams, Desmond

    2017-04-01

    Ebola virus is known to persist in semen of male survivors of Ebola virus disease (EVD). However, maximum duration of, or risk factors for, virus persistence are unknown. We report an EVD survivor with preexisting HIV infection, whose semen was positive for Ebola virus RNA 565 days after recovery from EVD.

  12. First successful artificial insemination with frozen-thawed semen in rhinoceros.

    Science.gov (United States)

    Hermes, R; Göritz, F; Saragusty, J; Sós, E; Molnar, V; Reid, C E; Schwarzenberger, F; Hildebrandt, T B

    2009-02-01

    The first successful artificial insemination (AI) in a rhinoceros was reported in 2007 using fresh semen. Following that success, we decided to evaluate the possibility of using frozen-thawed semen for artificial insemination. Semen, collected from a 35-36 year old Southern white rhinoceros (Ceratotherium simum simum) in the UK was frozen using the directional freezing technique. This frozen semen was used in two intrauterine AI attempts on a 30 years old female rhinoceros in Hungary. The first attempt, conducted 30 days postpartum with an insemination dose of approximately 135 x 10(6) motile cells, failed. The second attempt, conducted two estrus cycles later with an insemination dose of approximately 500 x 10(6) motile cells, resulted in pregnancy and the birth of a healthy offspring. This represents the first successful AI using frozen-thawed semen in a rhinoceros, putting it among very few wildlife species in which AI with frozen-thawed semen resulted in a live birth. The incorporation of AI with frozen-thawed semen into the assisted reproduction toolbox opens the way to preserve and transport semen between distant individuals in captivity or between wild and captive populations, without the need to transport stressed or potentially disease carrying animals. In addition, cryopreserved spermatozoa, in combination with AI, are useful methods to extend the reproductive lifespan of individuals beyond their biological lifespan and an important tool for managing genetic diversity in these endangered mammals.

  13. Sexed-semen usage for Holstein AI in the United States

    Science.gov (United States)

    The dairy industry has used sexed-semen to reduce the birth of undesirable bull calves for over a decade. While the efficacy of sexed-semen has been determined experimentally, we sought to tabulate statistics on the generalized use of the technology in the US dairy herd and determine its effectivene...

  14. Caffeine intake and semen quality in a population of 2,554 young Danish men

    DEFF Research Database (Denmark)

    Jensen, Tina Kold; Swan, Shanna H; Skakkebaek, Niels E

    2010-01-01

    The authors examined the association between semen quality and caffeine intake among 2,554 young Danish men recruited when they were examined to determine their fitness for military service in 2001-2005. The men delivered a semen sample and answered a questionnaire including information about...

  15. Ebola Virus RNA in Semen from an HIV-Positive Survivor of Ebola

    OpenAIRE

    Purpura, Lawrence J.; Rogers, Emerson; Baller, April; White, Stephen; Soka, Moses; Choi, Mary J.; Mahmoud, Nuha; Wasunna, Christine; Massaquoi, Moses; Kollie, Jomah; Dweh, Straker; Bemah, Philip; Ladele, Victor; Kpaka, Jonathan; Jawara, Mary

    2017-01-01

    Ebola virus is known to persist in semen of male survivors of Ebola virus disease (EVD). However, maximum duration of, or risk factors for, virus persistence are unknown. We report an EVD survivor with preexisting HIV infection, whose semen was positive for Ebola virus RNA 565 days after recovery from EVD.

  16. The Semen of Fertile Men in Jos, Nigeria | Akande | Journal of ...

    African Journals Online (AJOL)

    The objectives of the study was to assess decline in semen concentration in apparently fertile healthy men in Jos, Nigeria. During the period of the study, semen samples were obtained from men of proven fertility. These consisted 106 men who were male partners of pregnant women drawn from antenatal clinics. Apparently ...

  17. Factors associated with the determination of antibiotic activity in bovine semen.

    Science.gov (United States)

    Truscott, R B

    1983-10-01

    Rosaramicin, an agent shown to be effective in vitro against ureaplasma of bovine origin was tested as an additive to bovine semen extender. Although some reduction in semen quality occurred it was still deemed satisfactory for use. In a test involving 41 cows inseminated once at estrus with rosaramicin-treated semen (162 mcg/mL) the nonreturn rate was 24% compared to a calculated average for this semen of 63% (n = 3310). The effect of centrifugation, time and temperature was examined in vitro using a combination of 150 mcg of lincomycin, 300 mcg of spectinomycin and 450 mcg of tylosin against ten strains of bovine ureaplasma. This combination has ureaplasmacidal activity and is suggested as an additive to semen extenders for the control of ureaplasma.

  18. Air pollution and decreased semen quality: a comparative study of Chongqing urban and rural areas.

    Science.gov (United States)

    Zhou, Niya; Cui, Zhihong; Yang, Sanming; Han, Xue; Chen, Gangcai; Zhou, Ziyuan; Zhai, Chongzhi; Ma, Mingfu; Li, Lianbing; Cai, Min; Li, Yafei; Ao, Lin; Shu, Weiqun; Liu, Jinyi; Cao, Jia

    2014-04-01

    To investigate the association and effects of air pollution level on male semen quality in urban and rural areas, this study examines the outdoor concentrations of particulate matter (PM10), sulfur dioxide (SO2), nitrous dioxide (NO2) and semen quality outcomes for 1346 volunteers in both urban and rural areas in Chongqing, China. We found the urban area has a higher pollution level than the rural area, contrasted with better semen quality in the rural residents, especially for sperm morphology and computer assistant semen analysis (CASA) motility parameters. A multivariate linear regression analysis demonstrates that concentrations of PM10, SO2, and NO2 significantly and negatively are associated with normal sperm morphology percentage (P urban ambient air may account for worse semen quality in urban males. Copyright © 2014 Elsevier Ltd. All rights reserved.

  19. Does last week's alcohol intake affect semen quality or reproductive hormones?

    DEFF Research Database (Denmark)

    Hansen, M L; Thulstrup, A M; Bonde, J P

    2012-01-01

    The association between last 5 days of alcohol intake, semen quality and reproductive hormones was estimated in this cross-sectional study among 347 men. Conventional semen characteristics, DNA fragmentation index and reproductive hormones (testosterone, estradiol, sex hormone-binding globulin...... (SHBG), follicle-stimulating hormone (FSH), luteinizing hormone (LH) and inhibin B) were determined. There was a tendency towards lower semen characteristics at higher intake of alcohol past 5 days, albeit with no statistically significant dose-response association. The ratio between free estradiol...... and free testosterone was higher at higher alcohol intake during the 5 days preceding semen sampling. In conclusion, alcohol intake was associated with impairment of most semen characteristics but without a coherent dose-response pattern. The study indicates an association between recent alcohol intake...

  20. Nuclear Medicine

    Science.gov (United States)

    ... Parents/Teachers Resource Links for Students Glossary Nuclear Medicine What is nuclear medicine? What are radioactive tracers? ... funded researchers advancing nuclear medicine? What is nuclear medicine? Nuclear medicine is a medical specialty that uses ...

  1. Study on the Effect of Adding Bovine Serum Albumin to Semen Diluent on the Viability of Awassi Ram Semen Preserved at 5oC

    Directory of Open Access Journals (Sweden)

    Osama Ibrahim Azawi

    2011-10-01

    Full Text Available This study was aimed to test the effect of adding bovine serum albumin (BSA to Tris-fructose-egg yolk diluent to increase the ram sperm storage period at 5ºC. Semen samples from six mature Awassi rams used in this study. Semen samples were diluted by Tris-glucose-egg yolk. Diluted semen sample was divided into two parts. The first part BSA was added 10 mg ml-1 and the second part considered as a control group without any addition. The diluted semen samples were cooled and preserved at 5oC for 5 days. Cooled diluted semen samples were examined for individual motility, percent of live sperm, sperm abnormalities and acrosomal defects every 24 h until 5 days. Results of the present study showed an increased viability of spermatozoa diluted in the Tris diluent containing BSA stored at 5oC for 120 h of preservation compared with the control group. Spermatozoa motility and viability declined gradually in BSA Tris containing diluent when preserved at 5oC from 0 h to 120 h to 54.0±0.9% and 59.0±0.9 %, respectively compared to control of Tris diluent without BSA which was declined markedly to 35.9±1.9 %, 40.9±1.8 %, respectively. In conclusion, this study demonstrated that supplementation with BSA of ram semen diluents during semen preservation at 5oC, may exert beneficial effects on the quality of the Awassi ram semen.

  2. Deep-freezing of boar semen in plastic film 'cochettes'.

    Science.gov (United States)

    Eriksson, B M; Rodriguez-Martinez, H

    2000-03-01

    The motility and membrane integrity of spermatozoa from nine boars frozen with a programmable freezing machine in plastic bags, 'cochettes', and in 'maxi-straws', in total doses of 5 x 10(9) spermatozoa/5 ml with glycerol (3%) used as cryoprotectant, were assessed after thawing. A computer-based cell motion analyser was used to evaluate sperm motility, while the integrity of the plasmalemma was assessed with fluorescent supravital dyes (C-FDA/PI). The fertilizing capacity of the semen frozen in the two containers was investigated by inseminating (AI) gilts. Pregnancy was monitored by Doppler-ultrasound, and the numbers of corpora lutea and viable embryos counted at slaughter, between days 30 and 38 after AI. The cochettes sustained the overall procedure of freezing/thawing (FT), with 30 min post-thaw (PT) sperm motility being significantly higher than for straws, 46.9 vs. 39.5%. The only significant difference in motility patterns detected when comparing the packages was a higher sperm velocity (VCL) in cochettes at 30 min PT. However, percentages of FT-spermatozoa with intact membranes, detected with the supravital probes, were higher in maxi-straws than in cochettes, 46.8 vs. 43.0% (P straws and those frozen in cochettes. The results indicate that although the deep-freezing of AI-doses of boar semen in large plastic bags is feasible, problems such as their inconvenient size for storage and inconsistent thawing must be solved before this type of container can be used for the commercial cryopreservation of boar semen.

  3. Cryopreservation of boar semen in mini- and maxi-straws.

    Science.gov (United States)

    Bwanga, C O; de Braganca, M M; Einarsson, S; Rodriguez-Martinez, H

    1990-10-01

    Split ejaculates from four boars were frozen with a programmable freezing machine, in mini- (0.25 ml) and maxi- (5 ml) plastic straws with an extender at either acidic (6.3) or alkaline (7.4) pH. Glycerol (3%) was used as cryoprotectant. The freezing of the semen was monitored by way of thermocouples placed in the straws. Post-thaw motility and acrosome integrity were evaluated; the latter using phase contrast microscopy, eosin-nigrosin stain and electron microscopy. Post-thaw sperm motility was significantly higher when semen was frozen in mini-straws than in maxi-straws. For the mini-straws, the motility was better when semen was exposed to an acidic environment during freezing, but this beneficial effect of the low extracellular pH was not evident when maxi-straws were thawed. The motility of the spermatozoa diminished significantly during the thermoresistance test (0 h and 2 h time) at 37 degrees C in a similar way for both straws and extracellular pH's. The freezing procedure, no matter the extracellular pH, did not cause major acrosomal damages, but significantly more normal apical ridges were present in the mini-straws than in the maxi-straws. This in vitro evaluation indicated that the freezing method employed was better for mini- than for maxi-straws since the freezing of the 5 ml volumes was not homogeneous, due to the large section area between the surface and the core of the straw.

  4. Freezing African elephant semen as a new population management tool.

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    Robert Hermes

    Full Text Available BACKGROUND: The captive elephant population is not self-sustaining and with a limited number of breeding bulls, its genetic diversity is in decline. One way to overcome this is to import young and healthy animals from the wild. We introduce here a more sustainable alternative method - importation of semen from wild bulls without removing them from their natural habitat. Due to the logistics involved, the only practical option would be to transport cryopreserved sperm. Despite some early reports on African elephant semen cryopreservation, the utility of this new population management tool has not been evaluated. METHODOLOGY/PRINCIPAL FINDINGS: Semen was collected by electroejaculation from 14 wild African savanna elephant (Loxodonta africana bulls and cryopreserved using the directional freezing technique. Sperm treatments evaluated included the need for centrifugation, the use of hen or quail yolk, the concentration of glycerol (3%, 5% or 7% in the extender, and maintenance of motility over time after thawing. Our results suggest that dilution in an extender containing hen yolk and 7% glycerol after centrifugation best preserved post-thaw sperm motility when compared to all other treatments (P≤0.012 for all. Using this approach we were able to achieve after thawing (mean ± SD 54.6±3.9% motility, 85.3±2.4% acrosome integrity, and 86.8±4.6% normal morphology with no decrease in motility over 1 h incubation at 37°C. Sperm cryopreserved during this study has already lead to a pregnancy of a captive female elephant following artificial insemination. CONCLUSIONS/SIGNIFICANCE: With working techniques for artificial insemination and sperm cryopreservation of both African and Asian elephants in hand, population managers can now enrich captive or isolated wild elephant populations without removing valuable individuals from their natural habitat.

  5. Effects of work and life stress on semen quality

    Science.gov (United States)

    Janevic, Teresa; Kahn, Linda G.; Landsbergis, Paul; Cirillo, Piera M.; Cohn, Barbara A.; Liu, Xinhua; Factor-Litvak, Pam

    2014-01-01

    Objective To evaluate associations between work-related stress, stressful life events, and perceived stress and semen quality. Design Cross-sectional analysis. Setting Northern California. Patient(s) 193 men from the Child Health and Development Studies evaluated between 2005–2008. Intervention(s) None. Main Outcome Measure(s) Measures of stress including job strain, perceived stress, and stressful life events; outcome measures of sperm concentration, percentage of motile sperm, and percentage of morphologically normal sperm. Result(s) We found an inverse association between perceived stress score and sperm concentration (estimated coefficient b = –0.09 × 103/mL; 95% confidence interval [CI] = –0.18, –0.01), motility (b = –0.39; 95% CI = –0.79, 0.01), and morphology (b = –0.14; 95% CI, –0.25, –0.04) in covariate-adjusted linear regression analyses. Men who experienced two or more stressful life events in the past year compared with no stressful events had a lower percentage of motile sperm (b= –8.22; 95% CI, –14.31, –2.13) and a lower percentage of morphologically normal sperm (b = –1.66; 95% CI, –3.35, 0.03) but a similar sperm concentration. Job strain was not associated with semen parameters. Conclusion(s) In this first study to examine all three domains of stress, perceived stress and stressful life events but not work-related stress were associated with semen quality. PMID:24856463

  6. Successful artificial insemination in the Asian elephant (Elephas maximus) using chilled and frozen-thawed semen

    Science.gov (United States)

    Thongtip, Nikorn; Mahasawangkul, Sittidet; Thitaram, Chatchote; Pongsopavijitr, Pornsawan; Kornkaewrat, Kornchai; Pinyopummin, Anuchai; Angkawanish, Taweepoke; Jansittiwate, Saran; Rungsri, Ronnachit; Boonprasert, Khajornpat; Wongkalasin, Warut; Homkong, Pongpon; Dejchaisri, Suthathip; Wajjwalku, Worawit; Saikhun, Kulnasan

    2009-01-01

    Background Artificial insemination (AI) using frozen-thawed semen is well established and routinely used for breeding in various mammalian species. However, there is no report of the birth of elephant calves following AI with frozen-thawed semen. The objective of the present study was to investigate the fertilizing ability of chilled and frozen-thawed semen in the Asian elephant following artificial insemination (AI). Methods Semen samples were collected by from 8 bulls (age range, 12-to 42-years) by manual stimulation. Semen with high quality were either cooled to 4°C or frozen in liquid nitrogen (-196°C) before being used for AI. Blood samples collected from ten elephant females (age range, 12-to 52-years) were assessed for estrus cycle and elephants with normal cycling were used for AI. Artificial insemination series were conducted during 2003 to 2008; 55 and 2 AI trials were conducted using frozen-thawed and chilled semen, respectively. Pregnancy was detected using transrectal ultrasonography and serum progestagen measurement. Results One female (Khod) inseminated with chilled semen became pregnant and gave birth in 2007. The gestation length was 663 days and the sex of the elephant calf was male. One female (Sao) inseminated with frozen-thawed semen showed signs of pregnancy by increasing progestagen levels and a fetus was observed for 5 months by transrectal ultrasonography. Conclusion This is the first report showing pregnancy following AI with frozen-thawed semen in the Asian elephant. Successful AI in the Asian elephant using either chilled or frozen-thawed semen is a stepping stone towards applying this technology for genetic improvement of the elephant population. PMID:19615097

  7. EFFECT OF TWO DIFFERENT ANTIBIOTIC COMBINATIONS ON FERTILITY OF FROZEN BUFFALO AND SAHIW AL BULL SEMEN

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    S.M.H. Andrabi, N. Ahmad, A. Abbas and M. Anzar

    2001-09-01

    Full Text Available This study was carried out to identify the suitable antibiotic combinations in semen extender for improvement in fertility of frozen semen of buffalo and cow (Sahiwal bulls to obtain better pregnancy rate through artificial insemination (AI. For this study eight first ejaculates, four each from a buffalo and a cow (Sahiwal bull were used. The ejaculates were split-sampled and diluted with Tris-citric acid extender (at 37°C; 50x 106 spermatozoa/mI, containing either SP (streptomycin 1000 ~g/ml and penicillin 1000 IU/ml or GTLS (gentamycin 500 µg/ml, Tylosin 100 µg/ml and linco-spectin 300/600 µg/ml. There was no difference in post-thaw motility for these samples. Fertility test based on 75-days first service pregnancy rate was determined under field conditions. A total of 400 inseminations were recorded, 200 for each buffalo and cow (Sahiwal with J 00 of each antibiotic combination, respectively. Fertility rates for SP-based frozen semen of buffalo bull were 41.66% and were 55.2% for GTLS-containing frozen semen, respectively. The results for GTLS were higher (P<0.0001 than SP. Similarly, fertility rates were higher (P<0.0001 for GTLS-based frozen semen of Sahiwal bull (78.78% than SP-containing frozen semen (69.6% of the same specie. Fertility rates also differed due to species of donor bulls. They were better (P<0.0001 for the frozen Sahiwal bull semen than that of the buffalo bull in both SP and GTLS- based frozen semen samples, respectively. In conclusion. seminal quality measured by field fertility trial indicated GTLS combination of antibiotics added to the semen extender was better for improvement in the fertility of frozen buffalo and Sahiwal bull semen, by yielding better pregnancy rates through AI.

  8. Zika virus in semen: a prospective cohort study of symptomatic travellers returning to Belgium

    Science.gov (United States)

    Huits, Ralph; De Smet, Birgit; Ariën, Kevin K.; Van Esbroeck, Marjan; Bottieau, Emmanuel

    2017-01-01

    Abstract Objective To prospectively monitor Zika viral loads in semen from Belgian travellers with confirmed Zika virus infection, who returned from the Americas during the 2016 Zika virus epidemic. Methods We recruited symptomatic travellers consulting our clinic and we confirmed infection with either reverse-transcriptase (RT) polymerase chain reaction (PCR) assay or virus neutralization test. The participants produced semen samples weekly, either at the clinic or at home. For the initial sample, the laboratory staff did a microscopy analysis if they received the sample within an hour of production. Using RT–PCR, we monitored Zika virus ribonucleic acid (RNA) loads in semen until we obtained two negative results. Findings We detected Zika virus RNA in nine of 15 participants’ semen, one of whom was vasectomized. The median time to loss of RNA detection in semen was 83 days after symptom onset (95% confidence interval, CI: 57−108). The longest duration of viral shedding in semen before obtaining the first negative RT–PCR result was 144 days after symptom onset. All of the 11 participants, for whom we microscopically analysed their semen, had presence of leukocytes, 10 showed haematospermia and six showed oligospermia. These abnormalities occurred irrespective of Zika virus detection in semen. Conclusion The majority of men in our study had detectable Zika virus RNA in their semen. We recommend that semen from Zika virus-infected men should be analysed with RT–PCR and that health professionals should advise infected men, even if they are vasectomized, about current recommendations for prevention of sexual transmission of the virus. PMID:29200521

  9. Kualitas Semen Beku Kuda dalam Pengencer Susu Skim dan Dimitropoulos dengan Dimetilformamida Sebagai Krioprotektan

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    I. Arifiantini

    2007-08-01

    Full Text Available Equine semen are far less tolerant in the freezing and thawing process than bull semen. The stallion spermatozoa are known susceptible to cold-shock relating with the content of their fatty acid on the plasma membrane. The extender is one of determining factors in the success of stallion semen cryopreservation, as an energy source and protector the cell from harmfull effect of cold shock. The common cryoprotective agent (CPA for mammalian spermatozoa was glycerol, but for stallion semen cryopreservation, dimethyl formamide (DMF was more suitable. This research was conducted to compare the success of the stallion semen cryopreservation in skim milk and dimitropoulos (DV extender with DMF as cryoprotectant. Semen from three sexualy mature stallions was collected twice a week using an artificial vagina. Semen was evaluated macro- and microscopically and then divided into two tubes, diluted each of them with skim milk dan DV extender (1:1, centrifuged at 3 000 rpm for 15 minutes. The supernatant was removed and the pellet (spermatozoa was re-diluted in skim DMF (SDMF and DVDMF extender with the concentration of spermatozoa was 200x106 ml-1. The semen then packed in 0.3ml minitub straw equilibrated for two hours at 4-5oC and frezee in liquid N2 vapor for 10 minutes. The assessment of sperm quality was conducted based on the percentage of sperm motility and viability. In this research, post-thawed semen in DVDMF showed the percentages of the motility (36.2% and the viability (59.3% higher (P<0.05 than SDMF (28.5 and 48.0 %. In conclusion, the DVDMF extender provided better post-thawed semen quality than SDMF.

  10. Successful artificial insemination in the Asian elephant (Elephas maximus using chilled and frozen-thawed semen

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    Wongkalasin Warut

    2009-07-01

    Full Text Available Abstract Background Artificial insemination (AI using frozen-thawed semen is well established and routinely used for breeding in various mammalian species. However, there is no report of the birth of elephant calves following AI with frozen-thawed semen. The objective of the present study was to investigate the fertilizing ability of chilled and frozen-thawed semen in the Asian elephant following artificial insemination (AI. Methods Semen samples were collected by from 8 bulls (age range, 12-to 42-years by manual stimulation. Semen with high quality were either cooled to 4°C or frozen in liquid nitrogen (-196°C before being used for AI. Blood samples collected from ten elephant females (age range, 12-to 52-years were assessed for estrus cycle and elephants with normal cycling were used for AI. Artificial insemination series were conducted during 2003 to 2008; 55 and 2 AI trials were conducted using frozen-thawed and chilled semen, respectively. Pregnancy was detected using transrectal ultrasonography and serum progestagen measurement. Results One female (Khod inseminated with chilled semen became pregnant and gave birth in 2007. The gestation length was 663 days and the sex of the elephant calf was male. One female (Sao inseminated with frozen-thawed semen showed signs of pregnancy by increasing progestagen levels and a fetus was observed for 5 months by transrectal ultrasonography. Conclusion This is the first report showing pregnancy following AI with frozen-thawed semen in the Asian elephant. Successful AI in the Asian elephant using either chilled or frozen-thawed semen is a stepping stone towards applying this technology for genetic improvement of the elephant population.

  11. LEONARDO DA VINCI AND THE ORIGIN OF SEMEN.

    Science.gov (United States)

    Noble, Denis; DiFrancesco, Dario; Zancani, Diego

    2014-12-20

    It is well known that Leonardo da Vinci made several drawings of the human male anatomy. The early drawings (before 1500) were incorrect in identifying the origin of semen, where he followed accepted teaching of his time. It is widely thought that he did not correct this mistake, a view that is reflected in several biographies. In fact, he made a later drawing (after 1500) in which the description of the anatomy is remarkably accurate and must have been based on careful dissection. In addition to highlighting this fact, acknowledged previously in only one other source, this article reviews the background to Leonardo's knowledge of the relevant anatomy.

  12. Reactive oxygen species measurement in neat and washed semen: comparative analysis and its significance in male infertility assessment.

    Science.gov (United States)

    Venkatesh, S; Shamsi, M B; Dudeja, S; Kumar, R; Dada, Rima

    2011-01-01

    Oxidative stress (OS) is a major concern in idiopathic male infertility as elevated levels of reactive oxygen species (ROS) in the semen is believed to adversely affect sperm functional competence and damage both nuclear and mitochondrial DNA. Therefore, identifying infertile men with seminal OS may be used as a valuable tool in the assessment of male infertility. Semen is a complex mixture of spermatozoa and seminal plasma which is rich in enzymatic and non-enzymatic antioxidants. However, the measurement of ROS in the semen and its application in male infertility assessment is poorly understood. The aim of the present study was to compare the significance of ROS measurement in washed and neat semen. The study included 65 infertile men with abnormal semen (SA) parameters, 17 infertile men with normal semen (NS) parameters and 43 fertile controls (FC). ROS levels in both washed and neat semen were measured by luminol-dependent chemiluminescence assay and the values were expressed as 10(4 )RLU per minute per 20 million spermatozoa. The levels of ROS in both washed and neat semen were found to be significantly higher (P neat semen 0.03, washed semen 0.24), whereas infertile men with SA group had the highest median ROS level (neat semen 3.44, washed semen 27.42). In all subjects, ROS levels were found to be higher in washed semen than in neat semen. A strong positive correlation (r = 0.847, P neat and washed semen was observed. Measurement of ROS in neat semen reflects the original oxidative status in the actual sperm microenvironment and is an easy, simple, inexpensive and rapid method compared with ROS measurement from washed semen. ROS measurement in washed semen is done to assess ROS levels following sperm processing as in cases opting for assisted conception. As both ROS values in neat and washed show a positive correlation, neat semen may be used as a valuable tool for identifying infertile men with seminal OS. ROS levels are elevated in nearly 70% men with

  13. The power of semen: Aristotle and some Galen’s fallacies

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    Andrey Darovskikh

    2017-01-01

    Full Text Available In this paper, I try to demonstrate how critical empiricism and philosophical reasoning intertwine with each other and affected the development of medicine. It is a case study considering the problems of generation and semen in the writings of Aristotle and Galen via relationship between such concepts as matter, form, movement, change, causes and some others. The main question addressed in the paper is the reason of Galen’s return to Hippocratic paradigm of two-semina (male and female. I argue that the reason is two-fold: 1 Different philosophical reasoning and erroneous understanding of some aspects of Aristotle’s embryological model by Galen. 2 Empirical discoveries, which proved to be wrong. I demonstrate that Galen’s understanding of form/matter relationship, and his view on matter as an underling principle conditioned his understanding of the notion of physical change, that allowed him to speak about conception only as quantitative mixture between equal substrata. Finally, I show that Galen’s view on teleology and his limited understanding of formal/final vs efficient causes and their relationship forced him to claim the inadequacy of Aristotle’s biology and necessitated Galen to introduce emendations in definitions of seminal faculties of genders and reproductive fluids.

  14. Withania somnifera Improves Semen Quality in Stress-Related Male Fertility.

    Science.gov (United States)

    Mahdi, Abbas Ali; Shukla, Kamla Kant; Ahmad, Mohammad Kaleem; Rajender, Singh; Shankhwar, Satya Narain; Singh, Vishwajeet; Dalela, Deepansh

    2009-09-29

    Stress has been reported to be a causative factor for male infertility. Withania somnifera has been documented in Ayurveda and Unani medicine system for its stress-combating properties. However, limited scientific literature is available on this aspect of W. somnifera. We undertook the present study to understand the role of stress in male infertility, and to test the ability of W. somnifera to combat stress and treat male infertility. We selected normozoospermic but infertile individuals (N = 60), further categorized in three groups: normozoospermic heavy smokers (N = 20), normozoospermics under psychological stress (N = 20) and normozoospermics with infertility of unknown etiology (N = 20). Normozoospermic fertile men (N = 60) were recruited as controls. The subjects were given root powder of W. somnifera at a rate of 5 g/day for 3 months. Measuring various biochemical and stress parameters before and after treatment, suggested a definite role of stress in male infertility and the ability of W. somnifera to treat stress-related infertility. Treatment resulted in a decrease in stress, improved the level of anti-oxidants and improved overall semen quality in a significant number of individuals. The treatment resulted in pregnancy in the partners of 14% of the patients.

  15. Withania somnifera Improves Semen Quality in Stress-Related Male Fertility

    Directory of Open Access Journals (Sweden)

    Abbas Ali Mahdi

    2011-01-01

    Full Text Available Stress has been reported to be a causative factor for male infertility. Withania somnifera has been documented in Ayurveda and Unani medicine system for its stress-combating properties. However, limited scientific literature is available on this aspect of W. somnifera. We undertook the present study to understand the role of stress in male infertility, and to test the ability of W. somnifera to combat stress and treat male infertility. We selected normozoospermic but infertile individuals (N = 60, further categorized in three groups: normozoospermic heavy smokers (N = 20, normozoospermics under psychological stress (N = 20 and normozoospermics with infertility of unknown etiology (N = 20. Normozoospermic fertile men (N = 60 were recruited as controls. The subjects were given root powder of W. somnifera at a rate of 5 g/day for 3 months. Measuring various biochemical and stress parameters before and after treatment, suggested a definite role of stress in male infertility and the ability of W. somnifera to treat stress-related infertility. Treatment resulted in a decrease in stress, improved the level of anti-oxidants and improved overall semen quality in a significant number of individuals. The treatment resulted in pregnancy in the partners of 14% of the patients.

  16. A comparison of conventional and computer-assisted semen analysis (CRISMAS software) using samples from 166 young Danish men

    DEFF Research Database (Denmark)

    Vested, Anne; Ramlau-Hansen, Cecilia; Bonde, Jens P

    2011-01-01

    The aim of the present study was to compare assessments of sperm concentration and sperm motility analysed by conventional semen analysis with those obtained by computer-assisted semen analysis (CASA) (Copenhagen Rigshospitalet Image House Sperm Motility Analysis System (CRISMAS) 4.6 software) us...... and motility analysis. This needs to be accounted for in clinics using this software and in studies of determinants of these semen characteristics.......The aim of the present study was to compare assessments of sperm concentration and sperm motility analysed by conventional semen analysis with those obtained by computer-assisted semen analysis (CASA) (Copenhagen Rigshospitalet Image House Sperm Motility Analysis System (CRISMAS) 4.6 software......) using semen samples from 166 young Danish men. The CRISMAS software identifies sperm concentration and classifies spermatozoa into three motility categories. To enable comparison of the two methods, the four motility stages obtained by conventional semen analysis were, based on their velocity...

  17. Semen culture and the assessment of genitourinary tract infections

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    Michael Solomon

    2017-01-01

    Full Text Available The male factor contributes approximately 50% to infertility-related cases in couples with an estimated 12%–35% of these cases attributable to male genital tract infections. Depending on the nature of the infection, testicular sperm production, sperm transport, and sperm function can be compromised. Yet, infections are potentially treatable causes of infertility. Male genital tract infections are increasingly difficult to detect. Moreover, they often remain asymptomatic (“silent” with the result that they are then passed on to the relevant sexual partner leading to fertilization and pregnancy failure as well as illness of the offspring. With the worldwide increasing problem of antibiotic resistance of pathogens, proper diagnosis and therapy of the patient is important. This testing, however, should include not only aerobic microbes but also anaerobic as these can be found in almost all ejaculates with about 71% being potentially pathogenic. Therefore, in cases of any indication of a male genital tract infection, a semen culture should be carried out, particularly in patients with questionable semen quality. Globally, an estimate of 340 million new infections with sexually transmitted pathogens is recorded annually. Among these, the most prevalent pathogens including Chlamydia trachomatis, Ureaplasma urealyticum, Neisseria gonorrhoeae, and Mycoplasma hominis. Escherichia coli are considered the most common nonsexually transmitted urogenital tract microbes. These pathogens cause epididymitis, epididymo-orchitis, or prostatitis and contribute to increased seminal leukocyte concentrations.

  18. 'Gift without a price tag': altruism in anonymous semen donation.

    Science.gov (United States)

    Yee, Samantha

    2009-01-01

    Commercialization of human gametes is now legally prohibited in Canada under the Assisted Human Reproduction Act 2004, making semen donation in Canada altruistic and anonymous by law. Donors must be altruistically motivated to donate gametes without receiving monetary rewards. Globally speaking, Canada is neither the first nor the only country in the world that has legislation to support altruistic gamete donation. Other countries have advocated similar systems either through legislative changes or implementation of best practice models. This paper is a review of literature assessing the differences in donation behaviours under paid and altruistic donation models. It provides contextual information of the current semen donation situation in Canada, while drawing upon relevant literature and research data from other countries as references. The author also attempts to re-conceptualize the meanings of altruism through exploring the complex interplay between psycho-social and institutional factors in influencing donors' behaviours. Although there is a substantial amount of research studying the impacts on donor recruitment when payment is withdrawn, very few research studies are found that focus on exploring altruistic donor recruitment strategies. It is unrealistic to expect the altruistic donation culture to emerge spontaneously in Canada without any multi-level efforts to coordinate the recruitment strategies. Research programmes are greatly needed to generate empirical knowledge that can guide the development of altruistic donor recruitment models geared to the current socio-cultural environment and legislative framework in Canada. The findings will be invaluable when the legislation comes up for parliamentary review in the near future.

  19. Impact of cell phone use on men's semen parameters.

    Science.gov (United States)

    Gutschi, T; Mohamad Al-Ali, B; Shamloul, R; Pummer, K; Trummer, H

    2011-10-01

    The objective of the present retrospective study was to report our experience concerning the effects of cell phone usage on semen parameters. We examined 2110 men attending our infertility clinic from 1993 to October 2007. Semen analysis was performed in all patients. Serum free testosterone (T), follicle stimulating hormone (FSH), luteinising hormone (LH) and prolactin (PRL) were collected from all patients. The information on cell phone use of the patients was recorded and the subjects were divided into two groups according to their cell phone use: group A: cell phone use (n = 991); group B: no use (n = 1119). Significant difference was observed in sperm morphology between the two groups. In the patients of group A, 68.0% of the spermatozoa featured a pathological morphology compared to only 58.1% in the subjects of group B. Patients with cell phone usage showed significantly higher T and lower LH levels than those who did not use cell phone. No significant difference between the two groups was observed regarding FSH and PRL values. Our results showed that cell phone use negatively affects sperm quality in men. Further studies with a careful design are needed to determine the effect of cell phone use on male fertility. © 2011 Blackwell Verlag GmbH.

  20. Association of GH Gene Polymorphism with Semen Parameters of Boars

    Directory of Open Access Journals (Sweden)

    M. Kmieć

    2007-01-01

    Full Text Available Relations between polymorphism of the Growth Hormone gene and semen characters were analyzed. The DNA for the purpose of examination was isolated from the peripheral blood of 173 boars. In the boar herd under study the frequency of allele occurrence for the GH/MspI was as follows: allele GHA - 0.79 and allele GHB - 0.21. As far as the GH/HaeII polymorphism is concerned, the relevant frequency was as follows: allele GHA - 0.53 and allele GHB - 0.47, respectively. The relationship between the GH genotypes and semen characteristic traits were analyzed. The study showed that boars with GHBGHB genotype of both polymorphous loci of the GH gene produced ejaculates of larger volume, higher percentage, number of normozosperms in the ejaculate and number of insemination as compared to GHA GHA and GHAGHB boars. Our current findings suggested that polymorphism of the GH/MspI and GH/HaeII might have potential effect for reproductive performance traits of boars.

  1. Fixed-time insemination with frozen semen in mares: is it suitable for poorly fertile stallions?

    Science.gov (United States)

    Avanzi, Bruno Ribeiro; Ramos, Renata Dos Santos; Araujo, Gustavo Henrique Marques; Fioratti, Eduardo Gorzoni; Trinca, Luzia Aparecida; Dell'Aqua, José Antonio; Melo E Oña, Cely Marini; Zahn, Fabíola Soares; Martin, Ian; Alvarenga, Marco Antonio; Papa, Frederico Ozanam

    2015-06-01

    The purpose of the present study was to compare two protocols for equine frozen semen programs using either postovulation insemination or fixed-time insemination (FT), evaluating both pregnancy rates and intrauterine fluid (IUF) accumulation after artificial insemination with semen obtained from either highly or poorly fertile stallions. Six ejaculates from two stallions (n = 12) were processed. After thawing, semen samples were evaluated by computerized semen analysis. Fifteen mares (30 cycles) were inseminated with frozen semen from highly fertile stallion A, and 14 mares (28 cycles) were inseminated with frozen semen from poorly fertile stallion B. Ovulations were induced with 1 mg (intramuscular) of deslorelin acetate after the observation of a greater than 35 mm follicle and uterine edema. In postovulation insemination group, mares were inseminated once with 800 × 10(6) total sperm in a maximum 6-hour interval after ovulation. In FT group, mares were inseminated twice with 400 × 10(6) total sperm, 24 and 40 hours after induction. Mares were ultrasonographically examined for IUF accumulation 24 hours and for pregnancy diagnosis 14 days after the last insemination. Although IUF accumulation was more evident in mares inseminated once postovulation, pregnancy rates were similar for both protocols, regardless of the stallion, although a significant effect of the stallion was observed. These results indicated that FTs may be used for both highly and poorly fertile stallions as a practical tool to help spreading the use of frozen semen in equine reproduction programs. Copyright © 2015 Elsevier Inc. All rights reserved.

  2. Studi Awal Desain Pabrik Semen Portland dengan Waste Paper Sludge Ash sebagai Bahan Baku Alternatif

    Directory of Open Access Journals (Sweden)

    Rasdiana Rahma Nur

    2015-12-01

    Full Text Available Industri semen di Indonesia telah mengalami perkembangan yang pesat dan penggunaan batu kapur sebagai bahan baku pembuatan semen juga semakin meningkat. Saat ini sudah banyak dilakukan eksplorasi dan eksploitasi gunung kapur. Penggunaan batu kapur untuk produksi semen merupakan penggunaan yang terbanyak hingga 87,4% dari konsumsi total. Oleh karena itu, saat ini banyak penelitian dilakukan untuk mencari bahan baku alternatif agar produksi semen tetap berjalan dengan baik. Secara garis besar, desain proses pembuatan semen Portland menggunakan bahan baku alternatif waste paper slaudge ash dibagi ke dalam tiga tahap. Tahap pertama adalah persiapan bahan baku terdiri atas penambangan dan proses penghancuran (crushing. Tahap kedua adalah pembakaran bahan baku dan klinkerisasi di dalam suspension preheater dan rotary kiln, dilanjutkan pendinginan di dalam cooler. Proses terakhir yaitu proses akhir (finishing dengan menambahkan zat aditif ke dalam klinker untuk membentuk produk semen Portland. Operasi pabrik direncanakan kontinyu 24 jam/hari selama 300 hari dalam setahun. Untuk memproduksi semen 2.000.000 ton/tahun diperlukan bahan baku sebanyak 2.074.971,46 ton/tahun. Pabrik direncanakan berumur 10 tahun dengan pay out time sebesar 3,6 tahun dan internal rate of return sebesar 33,7% dan interest 11% sehingga dari segi teknis dan ekonomi, pabrik ini layak untuk didirikan.

  3. NEW HUMAN SEMEN ANALYSIS SYSTEM (CASA USING MICROSCOPIC IMAGE PROCESSING TECHNIQUES

    Directory of Open Access Journals (Sweden)

    N M Chaudhari

    2016-11-01

    Full Text Available Computer assisted semen analysis (CASA helps the pathologist or fertility specialist to evaluate the human semen. Detail analysis of spermatozoa like morphology and motility is very important in the process of intrauterine insemination (IUI or In-vitro fertilization (IVF in infertile couple. The main objective for this new semen analysis is to provide a low cost solution to the pathologist and gynecologist for the routine raw semen analysis, finding the concentration of the semen with dynamic background removal and classify the spermatozoa type (grade according to the motility and structural abnormality as per the WHO criteria. In this paper a new system , computer assisted semen analysis system is proposed in which hybrid approach is used to identify the moving object, scan line algorithm is applied for confirmation of the objects having tails, so that we can count the actual number of spermatozoa. For removal of background initially the dynamic background generation algorithm is proposed to create a background for background subtraction stage. The standard data set is created with 40× and 100× magnification from the different raw semen s. For testing the efficiency of proposed algorithm, same frames are applied to the existing algorithm. Another module of the system is focused on finding the motility and Type classification of individual spermatozoa.

  4. Impact of collection method on assessment of semen HIV RNA viral load.

    Directory of Open Access Journals (Sweden)

    Brendan J W Osborne

    Full Text Available The blood HIV RNA viral load is the best-defined predictor of HIV transmission, in part due to ease of measurement and the correlation of blood and genital tract (semen or cervico-vaginal viral load, although recent studies found semen HIV RNA concentration to be a stronger predictor of HIV transmission. There is currently no standardized method for semen collection when measuring HIV RNA concentration. Therefore, we compared two collection techniques in order to study of the impact of antiretroviral therapy on the semen viral load.Semen was collected by masturbation from HIV-infected, therapy-naïve men who have sex with men (MSM either undiluted (Visit 1 or directly into transport medium (Visit 2. Seminal plasma was then isolated, and the HIV RNA concentration obtained with each collection technique was measured and corrected for dilution if necessary. Collection of semen directly into transport medium resulted in a median HIV RNA viral load that was 0.4 log10 higher than undiluted samples.The method of semen collection is an important consideration when quantifying the HIV RNA viral load in this compartment.

  5. Improvement of the Shami goat semen quality by adding bovine serum albumin

    Directory of Open Access Journals (Sweden)

    O.I. Azawi

    2014-06-01

    Full Text Available The present study was aimed to improve the quality of Shami goat semen diluted with Tris diluent by adding bovine serum albumin. In the current study, six male goats were used. Semen was collected using artificial vagina of one ejaculate per week of every male included in this study. This study was performed during the breeding season from 1 \\ 10 \\ 2012 to 1 \\ 12 \\ 2012. In this study, two semen diluents were use first; Tris- fructose- egg yolk 2.5% and second Tris - fructose - 2.5% egg yolk with 1% of bovine serum albumin. Diluted semen samples were cooled gradually and stored at 5 ° C. Cooled diluted semen samples were examined every 24 h of storage to 144 h. These tests includes the proportion of live sperm and the percentage of secondary abnormalities of the sperm, the percentage of sperm acrosomal defects and percentage of progressive motility using a computer-aided sperm analysis. These results showed that the addition of bovine serum albumin with egg yolk to semen of male goats led to improved qualities of semen significantly (P<0.05 including the proportion of live sperm and the percentage of secondary abnormalities of the sperm, the percentage of sperm acrosomal defects and percentage of progressive motility. It could be concluded from the results of the current study, the possibility of storing goat semen for more than six days with alive sperm of more than 50% and the percentage of the progressive motility of more than 40% when adding bovine albumin serum to dilute goat semen at 1% level and this result has not reached by any previous study.

  6. Twice daily collection yields greater semen output and does not affect male libido in the ostrich.

    Science.gov (United States)

    Bonato, Maud; Rybnik, Paulina K; Malecki, Irek A; Cornwallis, Charlie K; Cloete, Schalk W P

    2011-02-01

    The success of an artificial insemination program in ostriches is highly dependent on the yield of viable semen. We, therefore, tested how semen output is affected by three different collection frequencies: once every 2d (48h interval), daily (24h interval), and twice a day (6h interval). Ejaculates were collected from seven male ostriches (aged 2-4 years) for 10 consecutive days using the dummy female method. We assessed semen characteristics (sperm motility, volume, concentration, number of sperm per ejaculate and sperm viability) and male libido (the delay between the presentation of the dummy and ejaculation, and the willingness to mount the dummy). The total daily output of semen and the number of sperm were greater at the 6h collection interval than at the 24h or 48h interval while sperm motility and viability were not affected. At the 6h interval, the number of live normal sperm increased over the treatment period while the number of live abnormal sperm was reduced. Furthermore, the time that males took to mount the dummy and their willingness to copulate with the dummy were unaffected by collection frequency. Across males we observed great individual variation in both semen characteristics and libido suggesting there is the potential to increase the efficiency of semen collection by selecting superior males. These results indicate not only that two collections per day yield maximum semen output and may improve semen viability, but also that quantifying variation between males may help further increase semen collection efficiency. Copyright © 2011 Elsevier B.V. All rights reserved.

  7. Effect of preputial washing on bacterial load and preservability of semen in Murrah buffalo bulls

    Directory of Open Access Journals (Sweden)

    G. S. Meena

    2015-06-01

    Full Text Available Aim: To study the effect of preputial washing on bacterial load, preservability and semen quality in Murrah buffalo bulls Materials and Methods: A total of 36 collections of three Murrah buffalo bulls maintained at Artificial Breeding Research Centre, ICAR-National Dairy Research Institute, Karnal, were collected at weekly intervals from each bull without preputial washing and latter ejaculates from same bull with preputial washing by infusing normal saline (0.85%, KMnO4 (0.02% and savlon (2.0% to first, second and third bull, respectively. The microbial load and semen quality were evaluated during different hours of storage at refrigerated temperature (0, 24 and 48 h and after thrawing of cryopreserved (at −196°C semen. Results: The results of preservation of semen at refrigerated temperature showed that bacterial load was markedly lower in ejaculates of bulls subjected to preputial washing. Semen preserved at refrigerator temperature and cryopreserved, the effect of washing solution was significant for individual motility (IM, non-eosiniphilic count, hypo-osmotic swelling reactivity (HOST, total plate count (TPC and acrosome integrity. KMnO4 was found to be the best in lowering bacterial load, sperm abnormalities and in improving semen quality such as motility, non-eosinophilic count, HOST and acrosome integrity even up to 48 h of preservation and cryopreserved semen. Effect of duration of preservation and stage of cryopreservation was also significant for IM, non-eosiniphilic count, HOST, sperm abnormalities and acrosome integrity. Conclusion: Overall the results suggested that preputial washing with KMnO4 solution improved the semen quality and reduced microbial load of Murrah buffalo bull’s semen preserved at refrigerated temperature and cryopreservation.

  8. Cryopreserving turkey semen in straws and nitrogen vapour using DMSO or DMA: effects of cryoprotectant concentration, freezing rate and thawing rate on post-thaw semen quality.

    Science.gov (United States)

    Iaffaldano, N; Di Iorio, M; Miranda, M; Zaniboni, L; Manchisi, A; Cerolini, S

    2016-04-01

    1. This study was designed to identify a suitable protocol for freezing turkey semen in straws exposed to nitrogen vapour by examining the effects of dimethylacetamide (DMA) or dimethylsulfoxide (DMSO) as cryoprotectant (CPA), CPA concentration, freezing rate and thawing rate on in vitro post-thaw semen quality. 2. Pooled semen samples were diluted 1:1 (v:v) with a freezing extender composed of Tselutin diluent containing DMA or DMSO to give final concentrations of 8% or 18% DMA and 4% or 10% DMSO. The semen was packaged in 0.25 ml plastic straws and frozen at different heights above the liquid nitrogen (LN2) surface (1, 5 and 10 cm) for 10 min. Semen samples were thawed at 4°C for 5 min or at 50°C for 10 s. After thawing, sperm motility, viability and osmotic tolerance were determined. 3. Cryosurvival of turkey sperm was affected by DMSO concentration. Freezing rate affected the motility of sperm cryopreserved using both CPAs, while thawing rates showed an effect on the motility of sperm cryopreserved using DMA and on the viability of sperm cryopreserved using DMSO. Significant interactions between freezing rate × thawing rate on sperm viability in the DMA protocol were found. 4. The most effective freezing protocol was the use of 18% DMA or 10% DMSO with freezing 10 cm above the LN2 surface and a thawing temperature of 50°C. An efficient protocol for turkey semen would improve prospects for sperm cryobanks and the commercial use of frozen turkey semen.

  9. Mechanisms of the harmful effects of bacterial semen infection on ejaculated human spermatozoa: potential inflammatory markers in semen.

    Science.gov (United States)

    Fraczek, Monika; Kurpisz, Maciej

    2015-01-01

    The invasion of the male reproductive tract by microorganisms, and its subsequent consequences for sperm fertilizing potential, has been intensely discussed. The role of the bacteria that are responsible for the colonization and contamination of the male urogenital tract, rather than its infection, in diminished sperm parameters raises the most controversy. There are numerous premises suggesting that bacterial semen infection is associated with male infertility. However, the molecular mechanism by which the fertility is affected is complex and multifactorial, and still presents a puzzle. Some authors have suggested that direct interactions between bacteria and human spermatozoa facilitate sperm immobilization, affect sperm morphology, and thus weaken the ability of sperm to fertilize. On the other hand, the massive infiltration of activated leukocytes into the inflammatory site may be associated with impairment of sperm fertilizing potential, due to oxidative, apoptotic, and immune processes. This review presents current research trends and aims to summarize the present knowledge of semen inflammation and causative bacterial agents in the male urogenital tract, with its consequence on seminological parameters, and male fertility status.

  10. Semen quality, reproductive hormones and fertility of men operated for hypospadias

    DEFF Research Database (Denmark)

    Asklund, C; Jensen, Tina Kold; Main, K M

    2010-01-01

    The testicular function of men previously operated for hypospadias has been sparsely investigated. Therefore, we investigated semen quality and reproductive hormones of 92 men with isolated hypospadias (IH) and 20 with hypospadias and additional genital disorders (HAGD) and compared with similar...... may be because of psychosocial aspects, sexual dysfunction or reduced semen quality or a combination of these factors. Our results should be reassuring for patients with mild forms of IH and their relatives. They can be informed that hypospadias in such cases is not generally associated with poor...... semen quality. Particularly among patients with HAGD, several may, however, need fertility treatment to reproduce....

  11. Use of immobilized cryopreserved bovine semen in a blind artificial insemination trial.

    Science.gov (United States)

    Standerholen, Fride Berg; Waterhouse, Karin Elisabeth; Larsgard, Anne Guro; Garmo, Randi Therese; Myromslien, Frøydis Deinboll; Sunde, Jan; Ropstad, Erik; Klinkenberg, Geir; Kommisrud, Elisabeth

    2015-08-01

    To make timing of artificial insemination (AI) relative to ovulation less critical, methods for prolonging shelf life of spermatozoa in vivo after AI have been attempted to be developed. Encapsulation of sperm cells is a documented technology, and recently, a technology in which sperm cells are embedded in alginate gel has been introduced and commercialized. In this study, standard processed semen with the Biladyl extender (control) was compared with semen processed by sperm immobilization technology developed by SpermVital AS in a blind field trial. Moreover, in vitro acrosome and plasma membrane integrity was assessed and compared with AI fertility data for possible correlation. Semen from 16 Norwegian Red young bulls with unknown fertility was collected and processed after splitting the semen in two aliquots. These aliquots were processed with the standard Biladyl extender or the SpermVital extender to a final number of 12 × 10(6) and 25 × 10(6) spermatozoa/dose, respectively. In total, 2000 semen doses were produced from each bull, divided equally by treatment. Artificial insemination doses were set up to design a blinded AI regime; 5 + 5 straws from each extender within ejaculates in ten-straw goblets were distributed to AI technicians and veterinarians all over Norway. Outcomes of the inseminations were measured as 56-day nonreturn rate (NRR). Postthaw sperm quality was assessed by flow cytometry using propidium iodide and Alexa 488-conjugated peanut agglutinin to assess the proportion of plasma membrane and acrosome-intact sperm cells, respectively. In total, data from 14,125 first inseminations performed over a 12-month period, 7081 with Biladyl and 7044 with SpermVital semen, were used in the statistical analyses. There was no significant difference in 56-day NRR for the two semen categories, overall NRR being 72.5% and 72.7% for Biladyl and SpermVital, respectively. The flow cytometric results revealed a significant higher level of acrosome-intact live

  12. Genetic gain in dairy cattle populations is increased using sexed semen in commercial herds

    DEFF Research Database (Denmark)

    Sørensen, Morten Kargo; Andersen, Jakob Voergaard; Pedersen, Louise Dybdahl

    2011-01-01

    Using stochastic simulation, the effect of using sexed semen to cow dams (CD) in a dairy cattle breeding scheme, with or without use of multiple ovulation and embryo transfer (MOET) to bull dams (BD), on annual genetic gain at the population level was examined. Three levels of sexed semen were...... with 100 cows each. Each year 50 young bulls (YB), 10 active sires and 215 BD were selected on best linear unbiased prediction estimated breeding values by truncation selection across the simulated population, and the YB were tested within the population. Use of sexed semen alone gave a positive increase...

  13. Métodos de colección de semen en camélidos sudamericanos

    OpenAIRE

    Pacheco Curie, Joel Iván

    2008-01-01

    La colección de semen depende de una buena y constante producciónespermática para que la calidad del semen sea buena. Las técnicas decolección de semen están bastante desarrolladas en otros animales,especialmente en rumiantes domésticos en los cuales ya es unprocedimiento de rutina, pero en camélidos, dadas las especialescaracterísticas reproductivas, anatómicas y fisiológicas de estas especies, esta colección es bastante dificultosa y no existe un protocolo recomendado y una técnica optima, ...

  14. Prophylactic Neuroprotection of Total Glucosides of Paeoniae Radix Alba against Semen Strychni-Induced Neurotoxicity in Rats: Suppressing Oxidative Stress and Reducing the Absorption of Toxic Components.

    Science.gov (United States)

    Li, Shujuan; Chu, Yanjie; Zhang, Ruowen; Sun, Linjia; Chen, Xiaohui

    2018-04-20

    Strychnos alkaloids (SAs) are the main toxic constituents in Semen Strychni, a traditional Chinese medicine, which is known for its fatal neurotoxicity. Hence, the present study was carried out to evaluate the neurotoxicity induced by SAs and the pre-protective effects of the total glucosides of Paeoniae Radix Alba (TGP). An SA brain damage model was firstly established. The neurotoxicity induced by SAs and the pre-protective effects of TGP were confirmed by physical and behavioral testing, biochemical assay, and histological examination. Then, a liquid chromatography-tandem mass spectrometry method was developed and validated to investigate the time-course change and distribution of strychnine and brucine (two main SAs) in the brain after oral SA administration with or without TGP pretreatment. Biochemical analysis results indicated that TGP could ameliorate the oxidative stress status caused by SAs. Time-course change and distribution studies demonstrated that strychnine and brucine were rapidly absorbed into the brain, peaked early at 0.5 h, and were mainly located in the hippocampus and cerebellum. TGP showed a pre-protective effect against neurotoxicity by reducing the absorption of toxic alkaloids into the brain. These findings could provide beneficial information in facilitating future studies of Semen Strychni neurotoxicity and developing herbal medicines to alleviate neurotoxicity in the clinic.

  15. Prophylactic Neuroprotection of Total Glucosides of Paeoniae Radix Alba against Semen Strychni-Induced Neurotoxicity in Rats: Suppressing Oxidative Stress and Reducing the Absorption of Toxic Components

    Directory of Open Access Journals (Sweden)

    Shujuan Li

    2018-04-01

    Full Text Available Strychnos alkaloids (SAs are the main toxic constituents in Semen Strychni, a traditional Chinese medicine, which is known for its fatal neurotoxicity. Hence, the present study was carried out to evaluate the neurotoxicity induced by SAs and the pre-protective effects of the total glucosides of Paeoniae Radix Alba (TGP. An SA brain damage model was firstly established. The neurotoxicity induced by SAs and the pre-protective effects of TGP were confirmed by physical and behavioral testing, biochemical assay, and histological examination. Then, a liquid chromatography-tandem mass spectrometry method was developed and validated to investigate the time-course change and distribution of strychnine and brucine (two main SAs in the brain after oral SA administration with or without TGP pretreatment. Biochemical analysis results indicated that TGP could ameliorate the oxidative stress status caused by SAs. Time-course change and distribution studies demonstrated that strychnine and brucine were rapidly absorbed into the brain, peaked early at 0.5 h, and were mainly located in the hippocampus and cerebellum. TGP showed a pre-protective effect against neurotoxicity by reducing the absorption of toxic alkaloids into the brain. These findings could provide beneficial information in facilitating future studies of Semen Strychni neurotoxicity and developing herbal medicines to alleviate neurotoxicity in the clinic.

  16. Effect of tris-extender supplemented with various concentrations of strawberry (Fragaria spp. on bull semen preservability

    Directory of Open Access Journals (Sweden)

    El-Sheshtawy RI

    2018-03-01

    Full Text Available Objective: To evaluate effect of tris-extender supplemented with various concentrations of strawberry (Fragaria spp. on bull semen preservability. Methods: Pooled bull semen were extended with tris-citrate-fructose egg yolk diluent (control, 0% strawberry and various concentrations of tris strawberry (TSB (1%-6% to achieve 60 million motile spermatozoa per milliliter. Extended semen were subjected to semen freezing protocol. Semen assessment including motility, alive%, abnormality%, intact sperm membrane (hypo-osmotic swelling test and conception rate were carried out for both chilled and frozen semen. Results: Results showed that sperm motility after chilling was enhanced in groups treated with various concentrations of TSB from 1% to 5% and exhibited higher significance (P<0.000 1 at 6-day post-chilling. In frozen semen, 3%, 4%, 5% and 6% concentrations gave the best significance (P<0.000 1 on sperm motility in comparison with the control. Concentration 1% revealed the highest significance (P<0.000 1 on alive% as compared to the control. Hypoosmotic swelling test was maintained as the control. Concentration 3% gave the lowest significance (P<0.000 1 considering abnormality%. The conception rate upon using frozen semen in insemination showed higher conception rate in concentrations of 5% and 6% in cattle. Conclusions: It is concluded that 1%-5% concentrations of TSB ameliorate bull semen characteristics after chilling, and 3%-6% concentrations of TSB improve bull semen characteristics after freezing. Higher conception rate exists in 5% and 6% concentration of TSB.

  17. Parental age at delivery and a man's semen quality

    DEFF Research Database (Denmark)

    Priskorn, Lærke; Jensen, Tina K; Lindahl-Jacobsen, Rune

    2014-01-01

    due to infertility in their partnership. Men born from 1960 and delivering a semen sample until year 2000 were included. The men were linked to the Danish Civil Registration System to obtain information on parent's age at delivery. Logistic regression analyses were used to calculate odds ratios and 95...... the possibility that a trend may have been identified with a still larger sample. In addition, the Danish Civil Registration System is merely administrative and hence does not discriminate between biological and adopted children. However, the low rate of adoption (≈2%) suggests that misclassification would have......, the few statistically significant results are likely attributable to chance. LIMITATIONS, REASONS FOR CAUTION: Information regarding individual subject characteristics which may impact sperm production (i.e. smoking, BMI) were not available. While our sample size was large, we cannot exclude...

  18. Low semen quality and experiences of masculinity and family building

    DEFF Research Database (Denmark)

    Sylvest, Randi; Fürbringer, Jeanette Krogh; Pinborg, Anja

    2018-01-01

    and intentions about family formation. MATERIAL AND METHODS: Semi-structured qualitative interview study at the Fertility Clinic at Copenhagen University Hospital, Hvidovre, Denmark. Ten men with very poor semen quality initiating fertility treatment were interviewed between November 2014 and May 2015. Data were...... couples had conflicts and discussions because the women in general wanted to talk more about infertility than the men. The men focused on having a biological child. They wanted to focus on achieving biological parenthood and postpone consideration of other family building options such as adoption......INTRODUCTION: Infertility is a concern for men and women.There is limited knowledge on how male factor infertility affects the couple in fertility treatment. The aim of this study was to explore how severe male factor infertility affects men's sense of masculinity, the couple's relationship...

  19. Male Fertility and Reduction in Semen Parameters: A Single Tertiary-Care Center Experience

    Directory of Open Access Journals (Sweden)

    D. Milardi

    2012-01-01

    Full Text Available Background. Infertility is both a clinical and a public problem, affecting the life of the couple, the healthcare services, and social environment. Standard semen analysis is the surrogate measure of male fertility in clinical practice. Objective. To provide information about the relationship between semen parameters and spontaneous conception. Methods. We evaluated retrospectively 453 pregnancies that occurred among 2935 infertile couples evaluated at an infertility clinic of a tertiary-care university hospital, between 2004 and 2009. Results. Normal semen analysis was present only in 158 patients; 295 subfertile patients showed alterations in at least one seminal parameter. A reduction in all seminal parameters was observed in 41 patients. Etiological causes of male infertility were identified in 314 patients. Conclusion. Our data highlights the possibility of a spontaneous conception with semen parameters below WHO reference values. Therefore, we support the importance of defining reference values on a population of fertile men. Finally, we analyzed the related ethical issues.

  20. A novel method for semen collection and artificial insemination in large parrots (Psittaciformes)

    Science.gov (United States)

    Lierz, Michael; Reinschmidt, Matthias; Müller, Heiner; Wink, Michael; Neumann, Daniel

    2013-01-01

    The paper described a novel technique for semen collection in large psittacines (patent pending), a procedure which was not routinely possible before. For the first time, a large set of semen samples is now available for analysis as well as for artificial insemination. Semen samples of more than 100 psittacine taxa were collected and analysed; data demonstrate large differences in the spermatological parameters between families, indicating an ecological relationship with breeding behaviour (polygamous versus monogamous birds). Using semen samples for artificial insemination resulted in the production of offspring in various families, such as Macaws and Cockatoos, for the first time ever. The present technique represents a breakthrough in species conservation programs and will enable future research into the ecology and environmental factors influencing endangered species. PMID:23797622

  1. Male urinary biomarkers of antimicrobial exposure and bi-directional associations with semen quality parameters.

    Science.gov (United States)

    Smarr, Melissa M; Honda, Masato; Kannan, Kurunthachalam; Chen, Zhen; Kim, Sungduk; Louis, Germaine M Buck

    2018-02-21

    Antimicrobials including parabens, triclosan, and triclocarban have endocrine disrupting properties. Among 501 male partners of couples planning to become pregnant, preconception urinary biomarkers of parabens, triclosan and triclocarban exposure were quantified in spot urine samples. Men also provided two fresh semen samples collected approximately one month to undergo 24-h semen quality analysis. Linear mixed-effects models, adjusted for creatinine, race, age and body mass index, were utilized to assess the relationship between log transformed chemical concentrations rescaled by their standard deviations and semen parameters. Methyl, ethyl and butyl parabens, were associated with diminished sperm count and several sperm motility parameters. Hydroxylated paraben metabolites and triclosan were significantly positively associated with select semen quality parameters. Overall, our findings suggest that specific urinary parabens found in consumer goods (methyl, ethyl and butyl parabens) may adversely impact sperm quality parameters among reproductive-age male partners of couples trying for pregnancy. Published by Elsevier Inc.

  2. Effect of Air Space in Storage Vials on Motility of Spermatozoa in Chilled Buck Semen

    Directory of Open Access Journals (Sweden)

    Magnus Paul K and Lali F Anand 1

    Full Text Available This study was conducted in order to find out the effect of air space on the top of glass vial in which semen is stored, on the motility of spermatozoa. 45 samples collected from two bucks over a span of 6 months were used for experiment. Goat milk extender was the diluent used. Two ml each of diluted semen after noting their initial motility was stored in 2 ml and 5 ml vials. Samples were stored at 5°C and motility of spermatozoa noted at 24 and 48 hours. Semen without air space was found to preserve the motility better than semen with air space on 24 and 48 hours of incubation. This could be better attributed to reactive oxygen species production by the spermatozoa, but further investigation is needed in this aspect to confirm it. [Veterinary World 2010; 3(9.000: 421-423

  3. THE RESULTS OF BITCH ARTIFICIAL INSEMINATION AFTER THE USE OF TESTED FROZEN SEMEN

    Directory of Open Access Journals (Sweden)

    K. KOSINIAK-KAMYSZ

    2007-05-01

    Full Text Available Many problems in dog reproduction concern both dog male, its behaviour andsemen quality as well as the bitch which are connected with physiological factors asa time oestrus cycle, anatomical structure of reproductive organs, sexual behaviourand ovulation moment. The results of bitches’ artificial insemination (AI with theuse of frozen semen are lower in comparison to raw semen. In connection with thisthe research work was performed with an idea of explanation of the problemconnected to low effect of the use of dog frozen semen for AI. It was found that it ispossible to receive more satisfactory results (about 75% of pregnancy rate whendog semen is testified on the base of sperm concentration and motility and alkalinephosphatase activity (AP. On the other side it is necessary to perform bitchesexamination based on cytological and hormonal testes which allows establishing thepernicious time for AI.

  4. THE RESULTS OF BITCH ARTIFICIAL INSEMINATION AFTER THE USE OF TESTED FROZEN SEMEN

    Directory of Open Access Journals (Sweden)

    BITTMAR A.

    2007-01-01

    Full Text Available Many problems in dog reproduction concern both dog male, its behaviour andsemen quality as well as the bitch which are connected with physiological factors asa time oestrus cycle, anatomical structure of reproductive organs, sexual behaviourand ovulation moment. The results of bitches’ artificial insemination (AI with theuse of frozen semen are lower in comparison to raw semen. In connection with thisthe research work was performed with an idea of explanation of the problemconnected to low effect of the use of dog frozen semen for AI. It was found that it ispossible to receive more satisfactory results (about 75% of pregnancy rate whendog semen is testified on the base of sperm concentration and motility and alkalinephosphatase activity (AP. On the other side it is necessary to perform bitchesexamination based on cytological and hormonal testes which allows establishing thepernicious time for AI.

  5. The Effect of Phyto-Lecithin on Preservation and Cryopreservation of Semen: A Review

    Directory of Open Access Journals (Sweden)

    AS Aku

    2007-01-01

    Full Text Available Artificial insemination represents one of technologies in livestock reproduction that can be applied to cattle, sheep, goats and other livestock. Application of livestock reproduction technology includes artificial insemination to increase reproductive efficiency. Semen processing is one critical phase in an artificial insemination program. The use of animal origin ingredient for semen extenders, such as egg yolk and milk, presents a risk of microbial contamination, which lead to the search for alternatives. To increase standard of quality, researchers exploits phyto-lesitin for semen extender and the results showed no significant differences in motility, viability, and acrosomal status of spermatozoa with phyto-lesitin extender when compared to tris-egg yolk-containing extenders. (Animal Production 9(1: 49-52 (2007 Key Words : Phyto-Lechitin, preservation, cryopreservation, semen

  6. SEASONAL INFLUENCE ON SEMINAL PATTERN AND FREEZABILITY OF PANTANEIRO BULL SEMEN

    Directory of Open Access Journals (Sweden)

    Nathalia Hack Moreira

    2016-01-01

    Full Text Available The purpose of this paper was to investigate the effect of the season of the year on testicular morphology, fresh and frozen/thawed semen quality from Pantaneiro bulls bred in Brasilia, DF. Six bulls were submitted, once a month for one year, to evaluation of testicular measurements, semen collection using an artificial vagina and cryopreserved with medium containing Tris-based, egg  yolk and glycerol. The testicular length and volume were greater (P 0.05. In conclusion, Pantaneiro bulls, bred in the Midwest region of Brazil, can be used in natural mating programs in all seasons. Aiming to improve the Brazilian Animal Germplasm Bank with a higher number of samples collected, we suggest that the freezing of the semen is done between June and August, although there will be no losses of semen quality at any time. Keywords: bovine; conservation; cryopreservation; reproduction; seasonality.

  7. Effect of smoking on reproductive hormones and semen parameters of infertile Saudi Arabians

    Directory of Open Access Journals (Sweden)

    Haifa A Al-Turki

    2015-01-01

    Conclusions: This study shows that the effect of smoking is dramatic reduction in the hormonal levels and semen parameters. It is recommended that smoking men undergoing fertility treatment should stop smoking to increase their chances of having offspring.

  8. Is maternal obesity related to semen quality in the male offspring? A pilot study

    DEFF Research Database (Denmark)

    Ramlau-Hansen, Cecilia Høst; Nøhr, Ellen Aagaard; Thulstrup, A M

    2007-01-01

    of underweight, and 25 sons of overweight, mothers. RESULTS: Inhibin B decreased with increasing maternal BMI (P = 0.04) and the point estimates for sperm concentration, semen volume, percent motile sperm, testosterone and FSH suggested an impaired reproductive status among sons of overweight mothers, but none......BACKGROUND: Obesity is a strong predictor of fecundity and maternal obesity may well program semen quality during pregnancy, but to our knowledge, no published studies have evaluated this hypothesis. METHODS: From a Danish pregnancy cohort established in 1984-87, 347 out of 5109 sons were selected...... for a follow-up study conducted from February 2005 to January 2006. Semen and blood samples were analyzed for conventional semen characteristics and reproductive hormones and related to information on maternal pre-pregnant body mass index (BMI) that was available for 328 men. Of these, 34 were sons...

  9. Human semen quality in relation to dietary pesticide exposure and organic diet

    DEFF Research Database (Denmark)

    Juhler, R. K.; Larsen, S. B.; Meyer, Otto A.

    1999-01-01

    The objective of the study was to corroborate or refute the hypothesis that farmers having a high intake of organic grown commodities have a high semen quality due to their expected lower level of dietary pesticides intake. Food frequency data and semen were collected from 256 farmers (171......%), medium (M, 1-49%), or a high (H, 50-100%) proportion of the fruit and vegetables consumed. Farmers having a high relative intake of organically grown fruit and vegetables also had a high relative consumption of organically produced meat, milk, and bread, and differences were observed comparing the actual....../ml). The group of men without organic food intake had a significant lower proportion of morphologically normal spermatozoa, but in relation to 14 other semen parameters no significant differences were found between the groups. Intake of 40 individual pesticides was correlated with four semen parameters...

  10. PENGARUH KOMPOSISI BAHAN PENGISI STYROFOAM PADA PEMBUATAN BATAKO MORTAR SEMEN DITINJAU DARI KARAKTERISTIK DAN KUAT TEKAN

    Directory of Open Access Journals (Sweden)

    Heru Winarno

    2016-08-01

    Full Text Available Penelitian ini dimaksudkan untuk memanfaatkan limbah styrofoam untuk dijadikan sebagai alternatif salah satu bahan pengisi campuran pembuatan batako mortar semen. Dengan menganalisis variasi komposisi bahan pengisi styrofoam  untuk campuran mortar beton semen terhadap karakteristik dan kuat tekan yang dapat dimanfaatkan sebagai bahan bangunan dibandingkan batako biasa. Penelitian ini direncanakan dalam masa empat bulan, dengan menganalisis variasi komposisi bahan pengisi styrofoam  untuk campuran mortar beton semen terhadap karakteristik dan kuat tekan. Penelitian ini menggunakan metode eksprimen yang dilakukan di Laboratorium Uji Bahan dan Beton Jurusan Pendidikan Teknik Sipil dan Perencanaan Fakultas Teknik Universitas Negeri Makassar. Urgensi penelitian ini dengan memanfaatkan limbah styrofoam untuk dijadikan sebagai alternatif salah satu bahan pengisi campuran pembuatan batako mortar semen, dapat menekan kerusakan lingkungan dimana limbah styrofoam  merupakan salah satu bagian dari pencemaran lingkungan.

  11. High incidence of oligozoospermia and teratozoospermia in human semen infected with the aerobic bacterium Streptococcus faecalis.

    Science.gov (United States)

    Mehta, R H; Sridhar, H; Vijay Kumar, B R; Anand Kumar, T C

    2002-01-01

    Bacterial culture of semen samples from 100 male partners in infertile couples revealed the presence of aerobic bacteria in 49 cases. Streptococcus faecalis (Enterococcus) was isolated from 53%, micrococci species from 20% and alpha-haemolytic streptococci from 16% of the infected samples. The incidence of oligozoospermia and teratozoospermia was significantly (P faecalis than those whose semen samples contained micrococci or alpha-haemolytic streptococci or those that did not contain bacteria. The mean sperm concentration, as well as the mean percentage of morphologically normal spermatozoa, was significantly (P faecalis compared with that containing micrococci or alpha-haemolytic streptococci and the uninfected samples. There is a high incidence of semen infection with S. faecalis, and it is associated with compromised semen quality in terms of sperm concentration and morphology. The presence of micrococci or alpha-haemolytic streptococci does not appear to have any detrimental effect on sperm quality.

  12. 9 CFR 98.35 - Declaration, health certificate, and other documents for animal semen.

    Science.gov (United States)

    2010-01-01

    ... Australia and New Zealand is to be transferred to females in a flock that is listed in the Scrapie National... name of the person to whom the animal semen will be delivered, and the location of the place to which...

  13. Recent adverse trends in semen quality and testis cancer incidence among Finnish men

    DEFF Research Database (Denmark)

    Jørgensen, N; Vierula, M; Jacobsen, R

    2011-01-01

    Impaired semen quality and testicular cancer may be linked through a testicular dysgenesis syndrome of foetal origin. The incidence of testis cancer has been shown to increase among Finnish men, whereas there is no recent publication describing temporal trends in semen quality. Therefore, we...... carried out a prospective semen quality study and a registry study of testis cancer incidence among Finnish men to explore recent trends. A total of 858 men were investigated in the semen quality study during 1998-2006. Median sperm concentrations were 67 (95% CI 57-80) million/mL, 60 (51-71) and 48 (39...... included in the registry study, which confirmed the increasing incidence of testicular cancer in recent cohorts. These simultaneous and rapidly occurring adverse trends suggest that the underlying causes are environmental and, as such, preventable. Our findings necessitate not only further surveillance...

  14. Associations between dietary patterns and semen quality in men undergoing IVF/ICSI treatment

    NARCIS (Netherlands)

    Vujkovic, M.; Vries, de J.H.M.; Dohle, G.R.; Bonsel, G.J.; Lindemans, J.; Macklon, N.S.; Spek, van der P.J.; Steegers, E.A.P.; Steegers-Theunissen, R.P.M.

    2009-01-01

    BACKGROUND: This study investigates whether dietary patterns, substantiated by biomarkers, are associated with semen quality. METHODS: In 161 men of subfertile couples undergoing in vitro fertilization treatment in a tertiary referral clinic in Rotterdam, the Netherlands, we assessed nutrient

  15. Associations between dietary patterns and semen quality in men undergoing IVF/ICSI treatment

    NARCIS (Netherlands)

    Vujkovic, M.; de Vries, J. H.; Dohle, G. R.; Bonsel, G. J.; Lindemans, J.; Macklon, N. S.; van der Spek, P. J.; Steegers, E. A. P.; Steegers-Theunissen, R. P. M.

    This study investigates whether dietary patterns, substantiated by biomarkers, are associated with semen quality. In 161 men of subfertile couples undergoing in vitro fertilization treatment in a tertiary referral clinic in Rotterdam, the Netherlands, we assessed nutrient intakes and performed

  16. HPV Prophylactic Vaccination in Males Improves the Clearance of Semen Infection

    Directory of Open Access Journals (Sweden)

    Carlo Foresta

    2015-10-01

    Discussion: Humoral immunity has a major role in healing from HPV infection. Elder ART patients with HPV semen infection may benefit by the union of both specific counselling and available prophylactic vaccination.

  17. Semen quality: variations among fathers and effects of moderate alcohol drinking

    Directory of Open Access Journals (Sweden)

    Trevor G Cooper

    2015-02-01

    Full Text Available Semen analysis results from over 750 fathers in the USA demonstrated marked differences in the quality of semen from men at different locations and of different ethnic groups. Another paper failed to demonstrate any effects of moderate alcohol consumption during the week before provision of an ejaculate on semen quality and few on serum hormones, of over 8300 men in Europe and the USA. While these observations are interesting, the reasons for regional and ethnic differences in semen quality of fathers are unclear. Although, there was no attempt to confirm the participant-provided level of alcohol consumption, an increase in serum testosterone in the men at the higher end of alcohol intake is compatible with an alcohol effect on liver metabolism, although whether alcohol intake was the cause of higher testosterone, or men with higher androgen levels consume more alcohol, is not known.

  18. Cryopreservation of tambaqui (Colossoma macropomum) semen: extenders, cryoprotectants, dilution ratios and freezing methods.

    Science.gov (United States)

    Carneiro, P C F; Azevedo, H C; Santos, J P; Maria, A N

    2012-01-01

    The tambaqui is an Amazonian fish of great economic and environmental importance to Brazil and other South American countries. Several semen cryopreservation methodologies have been tested for different Brazilian fish species; however, there is little information on the use of this technique on tambaqui semen. The aim of the present study was to investigate the effect of osmolarity and activation solutions on sperm kinetics and, glucose solutions, cryoprotectants, dilution ratios, egg yolk and freezing methods on tambaqui semen freezing. The osmolarity of 230 mOsm was suitable for simultaneously yielding higher sperm motility (85%) and motility time (54 sec.) and osmolarities above 360 mOsm maintain immobile tambaqui sperm. The tambaqui semen can be successfully cryopreserved when diluted 1:9 in freezing medium composed of 5 percent glucose solution (290 mOsm) with 10 percent methylglycol and 5 percent egg yolk, and frozen directly in a dry shipper container.

  19. Serum lipid profiles are associated with semen quality

    Directory of Open Access Journals (Sweden)

    Chin-Yu Liu

    2017-01-01

    Full Text Available We aimed to explore the associations between different lipid profiles and semen quality in a large-scale general male population. Sperm concentration, total sperm motility, progressive motility, and normal sperm morphology of total 7601 participants were recorded. The association of these semen parameters with the triglyceride, total cholesterol, high-density lipoprotein, low-density lipoprotein, and very low-density lipoprotein of serum lipid profiles was analyzed. Sperm concentration was statistically positively correlated with triglyceride and very low-density lipoprotein (adjusted P = 0.001 and P = 0.005, respectively. Total sperm motility and progressive motility were statistically increased with increasing low-density lipoprotein and cholesterol levels (both adjusted P = 0.008 and P < 0.001, respectively. The similar J-shaped associations (high-low-low-high were noted between individual lipid profile and normal sperm morphology, especially low-density lipoprotein and cholesterol with statistical significance (adjusted P = 0.017 and P = 0.021, respectively. The prevalence of abnormal total sperm motility and progressive motility was decreased in participants with high levels of cholesterol (P = 0.008 and P = 0.019, respectively, and the reverse J-shaped associations (low-high-high-low were noted between high-density lipoprotein, triglyceride, very low-density lipoprotein, and the prevalence of abnormal normal sperm morphology (P = 0.010, P = 0.037, and P = 0.025, respectively. A high cholesterol level was associated with better sperm motility. Similar J-shaped associations were noted between all lipid profiles and normal sperm morphology; meanwhile, the reverse J-shaped trends were identified between them and abnormal normal sperm morphology prevalence.

  20. Spermatozoon ultrastructure and semen parameters of Brycon vermelha (Characiformes, Characidae).

    Science.gov (United States)

    Faustino, F; Silva, R C; Hilbig, C C; Makino, L C; Senhorini, J A; Ninhaus-Silveira, A; Nakaghi, L S O

    2015-06-01

    This study analyzed semen parameters and the ultrastructure of spermatozoa of Brycon vermelha. The semen was white and viscous, with a mean volume of 5.0±2.6 mL/kg body weight and mean spermatozoon concentration of 4.3±0.8×10(10) spermatozoa/mL. The estimated motility rate was 90%, with 50% of spermatozoa motile at 35.0±0.1 s and 100% immotile by 46.5±0.1 s. The spermatozoon of B. vermelha had a distinct head, midpiece, and flagellum. The ovoid head measured 1.9±0.2 μm by 1.3±0.1 μm, with its volume almost completely occupied by the nucleus, and was enveloped by an irregular nuclear membrane, with no acrosome vesicle. The nuclear fossa held the centriole complex and the initial segment of flagellum. The midpiece was symmetrical and measured 1.3±0.3μm. Mitochondria were scarce and restricted to the anterior region, while vesicles were absent. The posterior region of the midpiece was characterized by the absence of mitochondria and the presence of the cytoplasmic sheath. The flagellum, enclosed by the flagellar membrane, measured 29.6±3.4 μm, and possessed an axial filament containing a 9+2 microtubule pattern. The spermatozoa of B. vermelha appeared similar in structure to those of fish that breed through external fertilization, thus classifying them as uniflagellate anacrosomal aquasperm, or Type 1 aquasperm. Copyright © 2015 Elsevier B.V. All rights reserved.

  1. Methods of cryopreservation of Tambaqui semen, Colossoma macropomum.

    Science.gov (United States)

    Varela Junior, A S; Goularte, K L; Alves, J P; Pereira, F A; Silva, E F; Cardoso, T F; Jardim, R D; Streit, D P; Corcini, C D

    2015-06-01

    This study compared three different techniques for sperm cryopreservation of Tambaqui (Colossoma macropomum). Semen was diluted in Beltsville Thawing Solution with the addition of dimethyl sulfoxide (DMSO) at various concentrations (5%, 10%, 15% and 20%). Cryopreservation was performed using three methods: Box Conditioner Method with straws at a 5 cm distance from liquid nitrogen vapor (N2L); Dry Shipper Method placing the straws inside the machine; Vitrification Method placing the straws directly into N2L, amounting to 12 treatments (four DMSO concentrations×three freezing methods). The samples were evaluated for analysis of sperm quality in vivo and in vitro. Use of the Vitrification Method at different concentrations of DMSO provided the least values in the different evaluations. Fertilization, hatching rates and plasma membrane integrity using the Box Conditioner Method with 5% and 10% DMSO did not differ (P>0.05) but use of the concentration of 5% DMSO resulted in greater values than the other treatments (P<0.05) as well as for sperm motility and latency time (P<0.05), although sperm viability was superior using the Dry Shipper Method with 20% of the cryoprotectant. Mitochondrial functionality was impaired by use of the Vitrification Method with all DMSO concentration tested showing the most desirable values when the Box Conditioner Method was used with 5%, 10%, 15% DMSO and the Dry Shipper Method was used with 10% and 15% DMSO. Considering the variables evaluated, the use of the Box Conditioner Method is associated with enhanced Tambaqui semen quality with freeze concentrations of 5% and 10% DMSO. Copyright © 2015. Published by Elsevier B.V.

  2. The Type of Container and Filling Method Have Consequences on Semen Quality in Swine AI Doses

    Directory of Open Access Journals (Sweden)

    Iulian Ibanescu

    2016-05-01

    Full Text Available The automatic filling of semen doses for artificial insemination in swine shows economic advantages over the old-style, manual filling. However, no data could be found regarding the impact, if any, of this packing method on semen quality. This study aimed to compare two types of containers for boar semen, namely the automatically-filled tube and the manually-filled bottle, in terms of preserving the quality of boar semen. Five ejaculates from five different boars were diluted with the same extender and then divided in two aliquots. First aliquot was loaded in tubes filled by an automatic machine while the second was loaded manually in special plastic bottles. The semen was stored in liquid state at 17°C, regardless of the type of container and examined daily, for five days of storage by means of a computer-assisted sperm analyzer. Both types of containers maintained the semen within acceptable values, but after five days of storage significant differences (p<0.05 between the container types were observed in terms of all selected kinetic parameters. The tube showed better values for sperm motility and velocity, while the bottle showed superior values for straightness and linearity of sperm movement. The automatically-filled tubes offered better sperm motility in every day of the study. Given the fact that sperm motility is still the main criterion in assessing semen quality in semen production centers, the main conclusion of this study is that the automatic loading in tubes is superior and recommended over the old-style manual loading in bottles.

  3. THE EFFECT OF CAFFEINE ADMINISTRATION TO DILUTED SEMEN ON THE PROGENY IN RABBIT.

    OpenAIRE

    BODNAR, K.

    1998-01-01

    [EN] The effect of two caffeine preparations was studied on the reproductiva parameters (conception rate, litter size and weight, sex ratio of the offspring) in rabbits. In the experiment, Al was carried out on 78 lactating females (divided into 3 groups). Group A was inseminated with diluted semen containing 1 mg Coffeinum Natrium Benzoicum per millilitre, group B was inseminated with diluted semen containing 1 mg Coffeinum Natrium SaHcylicum per millílitre, group e was insemi...

  4. In vivo adverse effects of alpha-tocopherol on the semen quality of male bucks.

    Science.gov (United States)

    Majid, A; Qureshi, M S; Khan, R U

    2015-10-01

    Oxidative stress has detrimental effects on semen quality during spermatogenesis and semen processing for artificial insemination. This work was conducted to study the effect of different levels of vitamin E on the semen traits, oxidative status and trace minerals in Beetal bucks. Thirty-six bucks of similar body weight and age (1 year) were randomly divided into four groups. One group was kept as control with no supplementation (group 1), and the others were supplemented with 200 (group 2), 400 (group 3) and 800 IU (group 4) vitamin E/animal/day for 2 months. At the end of the experiment, semen samples were collected and evaluated. Seminal plasma was separated to study the concentration of superoxide dismutase (SOD), glutathione peroxidase (GPx), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and trace minerals (Zn, Cu, Mn and Fe). Group 3 showed significantly higher (p < 0.05) semen volume and per cent motility and lower dead sperm percentage compared to control group. Superoxide dismutase, GPx, Zn, Cu and Mn were higher in the same group. The level of AST decreased in group 3 without any change on the concentration of ALT. It is suggested that vitamin E at the rate of 400 IU/buck/day supported higher semen volume, per cent motility, per cent live spermatozoa, antioxidants (SOD, GPx) and trace mineral levels (Zn, Cu, Mn) in the seminal plasma. The increased supplementation from 0 to 400 showed a general increasing trend in improving semen quality. However, the dose of 800 IU/kg had no useful effect in further improving the semen quality. Journal of Animal Physiology and Animal Nutrition © 2015 Blackwell Verlag GmbH.

  5. Uncertainty associated with assessing semen volume: are volumetric and gravimetric methods that different?

    Science.gov (United States)

    Woodward, Bryan; Gossen, Nicole; Meadows, Jessica; Tomlinson, Mathew

    2016-12-01

    The World Health Organization laboratory manual for the examination of human semen suggests that an indirect measurement of semen volume by weighing (gravimetric method) is more accurate than a direct measure using a serological pipette. A series of experiments were performed to determine the level of discrepancy between the two methods using pipettes and a balance which had been calibrated to a traceable standard. The median weights of 1.0ml and 5.0ml of semen were 1.03 g (range 1.02-1.05 g) and 5.11 g (range 4.95-5.16 g), respectively, suggesting a density for semen between 1.03g and 1.04 g/ml. When the containers were re-weighed after the removal of 5.0 ml semen using a serological pipette, the mean residual loss was 0.12 ml (120 μl) or 0.12 g (median 100 μl, range 70-300 μl). Direct comparison of the volumetric and gravimetric methods in a total of 40 samples showed a mean difference of 0.25ml (median 0.32 ± 0.67ml) representing an error of 8.5%. Residual semen left in the container by weight was on average 0.11 g (median 0.10 g, range 0.05-0.19 g). Assuming a density of 1 g/ml then the average error between volumetric and gravimetric methods was approximately 8% (p gravimetric measurement of semen volume. Laboratories may therefore prefer to provide in-house quality assurance data in order to be satisfied that 'estimating' semen volume is 'fit for purpose' as opposed to assuming a lower uncertainty associated with the WHO recommended method.

  6. The measurement of reactive oxygen species in human neat semen and in suspended spermatozoa: a comparison.

    Science.gov (United States)

    Fingerova, Helena; Oborna, Ivana; Novotny, Jiri; Svobodova, Magda; Brezinova, Jana; Radova, Lenka

    2009-10-27

    It is generally accepted that oxidative stress is an important factor in male infertility because it may impair the physiological function of spermatozoa at the molecular level. Nevertheless, although several approaches have been reported, the imbalance between production of reactive oxygen species (ROS) and activity of the antioxidant defense system in semen is difficult to investigate and remains poorly understood. This study compares measurement of ROS production in neat semen and in washed spermatozoa obtained from the same ejaculate, and suspended in phosphate buffered saline using exactly the same luminol-mediated chemiluminescence method. Ninety one samples were obtained from males of infertile couples and 34 from volunteers with proven fertility. As expected, ROS levels were markedly lower in neat semen than in washed spermatozoa suspensions where seminal plasma with its potent antioxidant capacity was removed. In the cases of both neat semen and washed spermatozoa, ROS production was lowest in samples from normozoospermic males and highest in samples containing more than half million peroxidase-positive leukocytes per milliliter. For all samples, there was a significant positive correlation between ROS production by neat semen and that by washed spermatozoa suspension. Measurement of ROS production in neat semen better reflects actual oxidative status because it detects only the overproduction of ROS which are not effectively scavenged by antioxidant capacity of seminal fluid. The results of our study show a good commutability of both measurements for identification of semen samples with high ROS production. The measurement in neat semen is even less time consuming and therefore easier to implement into laboratory routine.

  7. The Association Between Testis Cancer and Semen Abnormalities Before Orchiectomy: A Systematic Review.

    Science.gov (United States)

    Djaladat, Hooman; Burner, Elizabeth; Parikh, Pooja M; Beroukhim Kay, Dorsa; Hays, Krystal

    2014-12-01

    Purpose: Testicular germ cell tumors (TGCT) are the most common solid organ malignancy in young men. It is a largely curable disease, so the extent to which it affects quality of life-including male fertility-is important. Abnormal semen analysis is highly predictive of male infertility. We conducted a systematic review of published studies that reported pre-orchiectomy semen parameters (as a surrogate for fertility) in TGCT patients to evaluate the association between TGCT and semen abnormalities before orchiectomy. Methods: We conducted a systematic review of peer-reviewed publications reporting semen parameters before orchiectomy in adult patients diagnosed with TGCT. Further, we assessed the association between TGCT and semen abnormalities that may lead to infertility. Results: We applied MeSH search terms to four online databases (PubMed, Cochrane Reviews, Web of Science, and Ovid), resulting in 701 potentially relevant citations. After conducting a three-stage screening process, six articles were included in the systematic review. For each study, the participants' data and the study's quality and risk of bias were assessed and described. All studies showed semen abnormalities-including count, motility, and morphology-in men with TGCT prior to orchiectomy. Conclusions: TGCT is associated with semen abnormalities before orchiectomy. This review shows an increase in abnormal semen parameters among men with TGCT even outside the treatment effects of orchiectomy, radiation, or chemotherapy. To improve long-term quality of life, these findings should be considered when counseling patients on future fertility and sperm banking during discussions about treatment and prognosis for TGCT.

  8. Characteristics of seminal plasma and cryopreservation of anoa (Bubalus sp. semen obtained by electroejaculation

    Directory of Open Access Journals (Sweden)

    Yudi

    2011-03-01

    Full Text Available The population of anoa, which is an endemic fauna to Indonesia, was getting decrease caused by the illegal hunting and deforestation. Anoa is included in endangered species by IUCN, and Appendix I by CITES. The experiment aimed to characterize the seminal plasma contents and to cryopreserve the anoa semen for artificial insemination application in captivity. The experiment was carried out in Taman Safari Indonesia (Bogor. Semen was collected from 2 anesthetized males (4-10 years by electroejaculation. Seminal plasma gained by centrifugation of ejaculate (3000 rpm, 20 minutes, and then was evaluated the biochemical contents. Other ejaculates were evaluated macroscopically and microscopically, and then extended in Tris and Na-citrate media to a total concentration of 100 billion cells mL-1. Extended semen was stored at 4oC, and evaluated the motility and viability every 12 h. Frozen semen was made in Tris medium added with 5% of glycerol. The seminal plasma of anoa contained total lipid, Na, Ca and Mg higher than the buffalo, but its total protein, K and Cl were lower. Electrophoresis of seminal plasma using by SDS-PAGE method showed 10 bands of proteins (17-148 kDa. The motility and viability of chilled-extended semen in Tris and Na-citrate media were not significantly different (P > 0.05 during 72 h of evaluation. Extended semen in both of media may applicable for AI program for 24-48 h. Post thawing motility of frozen semen was still low, 26.00 ± 9.62%. Therefore, it is necessary to improve each stages of semen processing, so the motility will increased and resulted high pregnancy in AI program.

  9. Cryobiology and a new look at the preservation of stallion semen

    DEFF Research Database (Denmark)

    Grout, Brian William Wilson; Lehn-Jensen, Henrik; Petersen, Morten Rønn

    2009-01-01

      During freeze-preservation of high-viability ejaculates of horse semen the duration of the equilibration time for nucleated straws (achieved at -7 0C following induced nucleation and during controlled, slow cooling to -60 0C) has little impact on viability, measured using propidium iodide stain...... is discussed and new approaches for successful cryopreservation of semen of low viability are considered.   Keywords Equine spermatozoa; Cryopreservation; Nucleation; Osmotic stress; Repeated freezing...

  10. Detection of atypical porcine pestivirus in semen from commercial boar studs in the United States.

    Science.gov (United States)

    Gatto, I R H; Arruda, P H; Visek, C A; Victoria, J G; Patterson, A R; Krull, A C; Schwartz, K J; de Oliveira, L G; Arruda, B L

    2018-04-01

    Atypical porcine pestivirus (APPV) has recently been identified as a cause of congenital tremor (CT) in pigs and has been detected in semen and preputial swabs from boars that were known to be clinically affected with CT. Accordingly, the objectives of this study were to 1) detect the presence of APPV in semen, preputial fluids and preputial swabs from adult boars by quantitative reverse transcription PCR (qRT-PCR) and 2) genetically characterize a subset of positive samples to better understand the ecology of APPV in commercial boar studs and the potential risk of transmission of APPV via semen. A total of 597 samples of semen, preputial fluid and preputial swabs each representing a different boar were obtained from four commercial boar studs located in three different states in the United States. Viral RNA was detected by qRT-PCR in 90 samples (15.08%; 90/597), with the greatest per cent positive from preputial swabs (23.81%; 5/21) followed by preputial fluid (22.81%; 26/114) and semen (12.91%; 59/457). The mean cycle quantification (Cq) between sample types was similar while eleven semen samples had Cq values lower than 27.0 corresponding to approximately 2 × 10 6  copies/ml. Based on phylogenetic analysis of the Npro gene, different viral strains can be on the same farm at the same and different times. This is the first report of detection of APPV in semen from commercial boar studs. Studies investigating the role of semen in the transmission of APPV and production of CT are needed. © 2017 Blackwell Verlag GmbH.

  11. Usefulness of an injectable anaesthetic protocol for semen collection through urethral catheterisation in domestic cats.

    Science.gov (United States)

    Pisu, Maria Carmela; Ponzio, Patrizia; Rovella, Chiara; Baravalle, Michela; Veronesi, Maria Cristina

    2017-10-01

    Objectives Although less often requested in comparison with dogs, the collection of semen in cats can be necessary for artificial insemination, for semen evaluation in tom cats used for breeding and for semen storage. Urethral catheterisation after pharmacological induction with medetomidine has proved to be useful for the collection of semen in domestic cats. However, most of the previously used protocols require the administration of high doses of medetomidine that can increase the risk of side effects, especially on the cardiovascular system. In routine clinical practice, one safe and useful injectable anaesthetic protocol for short-term clinical investigations or surgery in cats involves premedication with low intramuscular doses of dexmedetomidine with methadone, followed by intravenous propofol bolus injection. We aimed to assess the usefulness of this injectable anaesthetic protocol for semen collection, via urethral catheterisation, in domestic cats. Methods The study was performed on 38 purebred, adult cats, during the breeding season, and semen was collected via urethral catheterisation using an injectable anaesthesia protocol with methadone (0.2 mg/kg) and dexmedetomidine (5 µg/kg) premedication, followed by induction with propofol. Results The anaesthetic protocol used in the present study allowed the collection of large-volume semen samples, characterised by good parameters and without side effects. Conclusions and relevance The results from the present study suggest that the injectable anaesthetic protocol using methadone and dexmedetomidine premedication, followed by induction with propofol, could be suitable and safe for the collection of a good-quality semen sample, via urethral catheterisation, in domestic cats. It can therefore be used as an alternative to previous medetomidine-based sedation protocols.

  12. Quality analysis of commercial samples of Ziziphi spinosae semen (suanzaoren by means of chromatographic fingerprinting assisted by principal component analysis

    Directory of Open Access Journals (Sweden)

    Shuai Sun

    2014-06-01

    Full Text Available Due to the scarcity of resources of Ziziphi spinosae semen (ZSS, many inferior goods and even adulterants are generally found in medicine markets. To strengthen the quality control, HPLC fingerprint common pattern established in this paper showed three main bioactive compounds in one chromatogram simultaneously. Principal component analysis based on DAD signals could discriminate adulterants and inferiorities. Principal component analysis indicated that all samples could be mainly regrouped into two main clusters according to the first principal component (PC1, redefined as Vicenin II and the second principal component (PC2, redefined as zizyphusine. PC1 and PC2 could explain 91.42% of the variance. Content of zizyphusine fluctuated more greatly than that of spinosin, and this result was also confirmed by the HPTLC result. Samples with low content of jujubosides and two common adulterants could not be used equivalently with authenticated ones in clinic, while one reference standard extract could substitute the crude drug in pharmaceutical production. Giving special consideration to the well-known bioactive saponins but with low response by end absorption, a fast and cheap HPTLC method for quality control of ZSS was developed and the result obtained was commensurate well with that of HPLC analysis. Samples having similar fingerprints to HPTLC common pattern targeting at saponins could be regarded as authenticated ones. This work provided a faster and cheaper way for quality control of ZSS and laid foundation for establishing a more effective quality control method for ZSS. Keywords: Adulterant, Common pattern, Principal component analysis, Quality control, Ziziphi spinosae semen

  13. Semen parameters can be predicted from environmental factors and lifestyle using artificial intelligence methods.

    Science.gov (United States)

    Girela, Jose L; Gil, David; Johnsson, Magnus; Gomez-Torres, María José; De Juan, Joaquín

    2013-04-01

    Fertility rates have dramatically decreased in the last two decades, especially in men. It has been described that environmental factors as well as life habits may affect semen quality. In this paper we use artificial intelligence techniques in order to predict semen characteristics resulting from environmental factors, life habits, and health status, with these techniques constituting a possible decision support system that can help in the study of male fertility potential. A total of 123 young, healthy volunteers provided a semen sample that was analyzed according to the World Health Organization 2010 criteria. They also were asked to complete a validated questionnaire about life habits and health status. Sperm concentration and percentage of motile sperm were related to sociodemographic data, environmental factors, health status, and life habits in order to determine the predictive accuracy of a multilayer perceptron network, a type of artificial neural network. In conclusion, we have developed an artificial neural network that can predict the results of the semen analysis based on the data collected by the questionnaire. The semen parameter that is best predicted using this methodology is the sperm concentration. Although the accuracy for motility is slightly lower than that for concentration, it is possible to predict it with a significant degree of accuracy. This methodology can be a useful tool in early diagnosis of patients with seminal disorders or in the selection of candidates to become semen donors.

  14. Comparison of reactive oxygen species in neat and washed semen of infertile men.

    Science.gov (United States)

    Moein, Mohammad Reza; Vahidi, Serajedin; Ghasemzadeh, Jalal; Tabibnejad, Nasim

    2014-05-01

    Male are involved in near 50% of cases of infertility and reactive oxygen species (ROS) playing an important role in decreasing fertility potential. Accurate measurement of ROS seems to be important in evaluation of infertile male patients. To compare ROS measurement in neat and washed semen samples of infertile men and define the best method for evaluation of ROS in these patients. We measured the level of ROS in semen samples of thirty five non-azoospermic men with infertility. The semen samples were divided into two parts and the semen parameters and ROS levels in neat and washed samples were evaluated. We also evaluated the presence of pyospermia using peroxidase test. The differences regarding sperm count and quick motility were significant in neat and washed semen samples. The mean ROS level was significantly higher in neat samples compared with washed spermatozoa (7.50 RLU vs. 1.20 RLU respectively). Difference in ROS levels was more significant in patients with pyospermia compared to whom with no pyospermia (378.67 RLU vs. 9.48 RLU respectively). Our study confirmed that neat or unprocessed samples are better index of normal oxidative status of semen samples. Because we do not artificially add or remove factors that may play an important role in oxidative equilibrium status.

  15. Toxoplasma gondii transmission by artificial insemination in sheep with experimentally contaminated frozen semen.

    Science.gov (United States)

    Consalter, Angélica; Silva, Andressa F; Frazão-Teixeira, Edwards; Matos, Luis F; de Oliveira, Francisco C R; Leite, Juliana S; Silva, Franciele B F; Ferreira, Ana M R

    2017-03-01

    Toxoplasma gondii is a parasite considered one of the major causes of reproductive problems in sheep. Furthermore, the presence of the agent in ram semen urges the possibility of sexual transmission in this species. The aim of this study was to evaluate if ram's frozen semen spiked with T. gondii tachyzoites would be able to cause infection in sheep by laparoscopic artificial insemination (AI). Nine ewes tested seronegative to anti-T. gondii antibodies by the modified agglutination test (MAT) were superovulated and inseminated to collect embryos. Animals were divided into two groups: G1 (n = 5), ewes inseminated with semen containing 4 × 10 7 tachyzoites; and G2 (n = 4), ewes inseminated with tachyzoite-free semen (control group). To confirm infection, ewe's blood samples were collected on days -14, -7, 0, 7, 14, 21, 28, 35, 49 and 57 after AI for analysis by MAT and PCR. Tissue samples of these ewes were also collected for histopathology and immunohistochemistry (IHC). Seven days after AI, all ewes of group G1 had specific antibodies to T. gondii, while those of G2 were negative. Toxoplasma gondii DNA was detected in the blood of one ewe and parasites were observed in tissues of all five animals inseminated with contaminated semen, indicating that semen freezing protocol does not affect T. gondii transmission by artificial insemination in sheep. Copyright © 2016 Elsevier Inc. All rights reserved.

  16. Toxic environmental chemicals in human semen: analytical method and case studies.

    Science.gov (United States)

    Stachel, B; Dougherty, R C; Lahl, U; Schlösser, M; Zeschmar, B

    1989-01-01

    In 1982, 89 men from all over the FRG participated in an investigation of sperm density and the presence of selected persistent environmental chemicals in their semen. Semen concentrations for the following chemicals were measured: lead, cadmium, hexachlorobenzene (HCBC), a-hexachlorocyclohexane (a-BHC), DDT and metabolites, dieldrin and polychlorobiphenyls (PBC). Heavy metal analyses were performed by Zeeman-AAS with direct sample measurement. A method for the quantitative determination of chlorinated hydrocarbons in semen by liquid-liquid extraction, clean-up and quantitation by gas chromatography with an electron capture detector. Recovery rates ranged from 72 to 120%. Compound identification in samples was confirmed by negative chemical ionization mass spectrometry. Heavy metals and organochlorine compounds in semen were present in the same concentration range as in blood or other biofluids. Occupational exposure as well as extraordinary environmental exposure appeared to influence semen concentrations. Students in chemistry showed elevated levels of organochlorine compounds. Men who lived in the vicinity of an atmospheric source of heavy metals showed strikingly the vicinity of an atmospheric source of heavy metals showed strikingly elevated levels of semen lead and cadmium. We were unable to find a statistically significant correlation between sperm density and any of the variables examined in this study including: orgonochlorine compounds summed or individually; lead and/or cadmium; and tobacco consumption. Significant correlations were found between the simultaneous occurrence of lead and cadmium and between the simultaneous occurrence of HCB, DDT and DDE.

  17. Urinary inorganic arsenic concentrations and semen quality of male partners of subfertile couples in Tokyo.

    Science.gov (United States)

    Oguri, Tomoko; Yoshinaga, Jun; Toshima, Hiroki; Mizumoto, Yoshifumi; Hatakeyama, Shota; Tokuoka, Susumu

    2016-01-01

    Inorganic arsenic (iAs) has been known as a testicular toxicant in experimental rodents. Possible association between iAs exposure and semen quality (semen volume, sperm concentration, and sperm motility) was explored in male partners of couples (n = 42) who visited a gynecology clinic in Tokyo for infertility consultation. Semen parameters were measured according to WHO guideline at the clinic, and urinary iAs and methylarsonic acid (MMA), and dimethylarsinic acid concentrations were determined by liquid chromatography-hydride generation-ICP mass spectrometry. Biological attributes, dietary habits, and exposure levels to other chemicals with known effects on semen parameters were taken into consideration as covariates. Multiple regression analyses and logistic regression analyses did not find iAs exposure as significant contributor to semen parameters. Lower exposure level of subjects (estimated to be 0.5 μg kg(-1) day(-1)) was considered a reason of the absence of adverse effects on semen parameters, which were seen in rodents dosed with 4-7.5 mg kg(-1).

  18. Reducing Inter-Laboratory Differences between Semen Analyses Using Z Score and Regression Transformations

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    Esther Leushuis

    2016-12-01

    Full Text Available Background: Standardization of the semen analysis may improve reproducibility. We assessed variability between laboratories in semen analyses and evaluated whether a transformation using Z scores and regression statistics was able to reduce this variability. Materials and Methods: We performed a retrospective cohort study. We calculated between-laboratory coefficients of variation (CVB for sperm concentration and for morphology. Subsequently, we standardized the semen analysis results by calculating laboratory specific Z scores, and by using regression. We used analysis of variance for four semen parameters to assess systematic differences between laboratories before and after the transformations, both in the circulation samples and in the samples obtained in the prospective cohort study in the Netherlands between January 2002 and February 2004. Results: The mean CVB was 7% for sperm concentration (range 3 to 13% and 32% for sperm morphology (range 18 to 51%. The differences between the laboratories were statistically significant for all semen parameters (all P<0.001. Standardization using Z scores did not reduce the differences in semen analysis results between the laboratories (all P<0.001. Conclusion: There exists large between-laboratory variability for sperm morphology and small, but statistically significant, between-laboratory variation for sperm concentration. Standardization using Z scores does not eliminate between-laboratory variability.

  19. Effects of Thawing on Frozen Semen Quality of Limousin and Brahman Bulls

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    wc Pratiwi

    2009-01-01

    Full Text Available The success of Artificial Insemination (AI influenced by many factor, there are nutrition, body condition and post thawing motility (PTM. The PTM influenced by liquid N2 storage, equilibration temperature and handling straw. The purpose of this research to compare the effect of thawing duration to frozen semen quality of Limousin and Brahman. This research was done in BIBD, Agriculture Official of Blora, Central Java and Laboratory of Beef Cattle Station Research, Grati. As semen source is bull of Limousin and Brahman with age 2-3 years, body weight + 1200 kg. The data was observed such as: (1 pH, (2 Motility, (3 Live sperm, (4 Abnormality. The research use Randomized Complete Design (RCD one way there are time of thawing 0, 15, 30, 45 minutes with 10 time repetition. The result of this research showed that the highest motility and live sperm (P<0,05 at the treatment with the duration of thawing 0 minute, there are 41,50% and 66,50% (Limousin frozen semen; 40,00% and 39,58% (Brahman frozen semen. It was concluded that shortening the time of thawing could be repairing the PTM and S/C value. (Animal Production 11(1: 48-52 (2009 Key Words : semen quality, frozen semen, thawing

  20. Effect of a dietary antioxidant supplementation on semen quality in pony stallions.

    Science.gov (United States)

    Deichsel, K; Palm, F; Koblischke, P; Budik, S; Aurich, C

    2008-05-01

    Lipid peroxidation contributes to the damage of the sperm plasma membrane. In different species, dietary supplementation with antioxidants has been shown to improve semen quality. Therefore, we tested effects of dietary supplementation with antioxidants and l-carnitin on semen quality in Shetland pony stallions (n=6). Semen was collected twice a week over a time period of 16 weeks. From weeks 5 to 12, a special diet for stallions containing a variety of antioxidants (STALLION, Pavo Pferdenahrung GmbH, Goch, Germany; tocopherol 300 mg/day; ascorbic acid 300 mg/day; l-carnitin 4000 mg/day; folic acid 12 mg/day) was added to the basal diet (hay, mineral supplements, water). Ejaculates were evaluated for total sperm count, semen motility (percentage of totally and progressively motile spermatozoa, longevity for 24 h at 5 degrees C) and membrane integrity (SYBR-14/PI staining): All values given are means+/-S.E.M. No changes in motility, progressive motility and membrane integrity or semen longevity for 24 h were detected. A slight but significant reduction of morphologically abnormal spermatozoa was found (weeks 1-4: 43.7+/-7.1%; weeks 13-16: 39.4+/-7.2%, p<0.05). Results show that a supplementary diet with antioxidants in the given concentration and duration does not result in pronounced effects on semen quality of stallions. It is therefore questionable to support stallions with dietary antioxidants as long as they receive an adequately balanced basal diet.

  1. Vaginal swab specimen processing methods influence performance of rapid semen detection tests: a cautionary tale.

    Science.gov (United States)

    Hobbs, Marcia M; Steiner, Markus J; Rich, Kimberly D; Gallo, Maria F; Warner, Lee; Macaluso, Maurizio

    2010-09-01

    Detection of semen biomarkers in vaginal fluid can be used to assess women's recent exposure to semen. Quantitative tests for detection of prostate-specific antigen (PSA) perform well, but are expensive and require specialized equipment. We assessed two rapid immunochromatographic strip tests for identification of semen in vaginal swabs. We tested 581 vaginal swabs collected from 492 women. Vaginal secretions were eluted into saline, and PSA was measured using the quantitative IMx (Abbott Laboratories, Abbott Park, IL, USA) assay. Specimens were also tested using the ABAcard p30 test (Abacus Diagnostics, West Hills, CA, USA) for detection of PSA and RSID-Semen test (Independent Forensics, Hillside, IL, USA) for detection of semenogelin (Sg). Vaginal swab extraction using saline was compatible with direct assessment of vaginal swab eluates using ABAcard for PSA detection, but not for Sg detection using RSID. The rapid PSA test detected 91% of specimens containing semen compared to 74% by the rapid Sg test. Investigators are urged to optimize vaginal swab specimen preparation methods for performance of RSID or other tests to detect semen components other than PSA. Previously described methods for PSA testing are not uniformly applicable to other tests. Copyright 2010 Elsevier Inc. All rights reserved.

  2. Rapid quantification of semen hepatitis B virus DNA by real-time polymerase chain reaction

    Science.gov (United States)

    Qian, Wei-Ping; Tan, Yue-Qiu; Chen, Ying; Peng, Ying; Li, Zhi; Lu, Guang-Xiu; Lin, Marie C.; Kung, Hsiang-Fu; He, Ming-Ling; Shing, Li-Ka

    2005-01-01

    AIM: To examine the sensitivity and accuracy of real-time polymerase chain reaction (PCR) for the quantification of hepatitis B virus (HBV) DNA in semen. METHODS: Hepatitis B viral DNA was isolated from HBV carriers’ semen and sera using phenol extraction method and QIAamp DNA blood mini kit (Qiagen, Germany). HBV DNA was detected by conventional PCR and quantified by TaqMan technology-based real-time PCR (quantitative polymerase chain reaction (qPCR)). The detection threshold was 200 copies of HBV DNA for conventional PCR and 10 copies of HBV DNA for real time PCR per reaction. RESULTS: Both methods of phenol extraction and QIAamp DNA blood mini kit were suitable for isolating HBV DNA from semen. The value of the detection thresholds was 500 copies of HBV DNA per mL in the semen. The viral loads were 7.5 × 107 and 1.67 × 107 copies of HBV DNA per mL in two HBV infected patients’ sera, while 2.14 × 105 and 3.02 × 105 copies of HBV DNA per mL in the semen. CONCLUSION: Real-time PCR is a more sensitive and accurate method to detect and quantify HBV DNA in the semen. PMID:16149152

  3. INSEMINACIÓN ARTIFICIAL DE ALPACAS CON SEMEN COLECTADO POR ASPIRACIÓN VAGINAL Y VAGINA ARTIFICIAL.

    OpenAIRE

    Alarcón B., Virgilio; Centro Experimental La Raya, Universidad Nacional San Antonio Abad, Cusco, Perú; García V., Wilber; Centro de Investigación IVITA-Maranganí, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima-Perú.; Bravo, P. Walter; Carrera Profesional de Medicina Veterinaria, Canchis, Universidad Nacional San Antonio Abad, Cusco. Perú

    2012-01-01

    Semen de alpaca fue colectado por dos métodos: por aspiración de la vagina de la hembra después de la monta natural y con vagina artificial. El semen colectado fue evaluado y diluido con Tris tamponado, y luego usado en inseminacion artificial. Se trabajó con 160 alpacas hembras adultas de capacidad reproductiva comprobada y 5 alpacas machos. Se colectó semen post cópula de los cinco machos en 10 hembras, y se hicieron 50 colecciones de semen con vagina artificial de estos machos, dos veces p...

  4. Viability and fertility of cooled equine semen diluted with skimmed milk or glycine egg yolk-based extenders

    Directory of Open Access Journals (Sweden)

    Guilherme Pugliesi

    2012-12-01

    Full Text Available Two semen extenders were compared for their ability to maintain viability of horse semen during 24 hours of cold preservation, and for the pregnancy rate after artificial insemination. In the experiment 1, five ejaculates from three stallions were split-diluted in either a skimmed milk-based extender (Kenney extender or a glycine egg yolk-based extender (Foote extender and cooled at 6-8 ºC for 24 hours. Semen samples stored in Kenney extender for 24 hours had higher motility and spermatic vigor compared with those stored in Foote extender. However, samples stored in Foote extender had higher number of reactive sperm by hypoosmotic test and greater viability by epifluorescence test compared with those in Kenney extender. In the experiment 2, 17 and 23 ejaculates from two stallions were split-diluted with Kenney extender and Foote extender. The sperm concentration in each extender was adjusted to 500 million viable sperms per insemination dose. Semen was cooled to 6-8 ºC and stored for 24 hours. Seventy-four cycles of crossbred mares were inseminated with either semen diluted in Kenney extender or semen diluted in Foote extender. The pregnancy rate was higher from semen diluted in Kenney extender than that from semen in Foote extender (0.553 vs. 0.306. The Kenney extender is effective in preserving the motility, vigor and fertility of stallion semen after 24 hours of cold storage, whereas the Foote extender is not acceptable.

  5. Hair mercury (Hg) levels, fish consumption and semen parameters among men attending a fertility center.

    Science.gov (United States)

    Mínguez-Alarcón, Lidia; Afeiche, Myriam C; Williams, Paige L; Arvizu, Mariel; Tanrikut, Cigdem; Amarasiriwardena, Chitra J; Ford, Jennifer B; Hauser, Russ; Chavarro, Jorge E

    2018-03-01

    General population exposure to methylmercury (MeHg), the most common organic mercury compound found in the environment, occurs primarily through the consumption of contaminated fish and shellfish. Due to limited studies and lack of consideration of effect modification by fish consumption, it remains uncertain if exposure to mercury affects semen parameters. Thus, we investigated whether hair Hg levels, a biomarker of mercury exposure, were associated with semen parameters among men attending an academic fertility center, and whether this relationship was modified by intake of fish. This analysis included 129 men contributing 243 semen samples who were enrolled in the Environment and Reproductive Health (EARTH) Study between 2005 and 2013, and had data of hair Hg, intake of fish and semen parameters available. Hair Hg levels were assessed using a direct mercury analyzer. Intake of fish was collected using a validated food frequency questionnaire. Semen parameters were analyzed following WHO 2010 evaluation criteria. Generalized linear mixed models with random intercepts accounting for within-man correlations across semen samples were used to evaluate the association of hair Hg levels and semen parameters adjusting for age, BMI, smoking status, abstinence time and alcohol intake. Effect modification by total fish intake (≤1.68 vs. >1.68 servings/week) was tested. The median hair Hg levels of the men was 0.72ppm and ranged from 0.03 to 8.01ppm; almost 30% of the men had hair Hg levels >1ppm. Hair Hg levels were positively related with sperm concentration, total sperm count, and progressive motility, after adjusting for potential confounders and became attenuated after further adjustment for fish intake. Specifically, men in the highest quartile of hair mercury levels had 50%, 46% and 31% higher sperm concentration, total sperm count and progressive motility, respectively, compared to men in the lowest quartile. These associations were stronger among men whose fish

  6. Aerospace Medicine

    Science.gov (United States)

    Michaud, Vince

    2015-01-01

    NASA Aerospace Medicine overview - Aerospace Medicine is that specialty area of medicine concerned with the determination and maintenance of the health, safety, and performance of those who fly in the air or in space.

  7. Nuclear Medicine.

    Science.gov (United States)

    Badawi, Ramsey D.

    2001-01-01

    Describes the use of nuclear medicine techniques in diagnosis and therapy. Describes instrumentation in diagnostic nuclear medicine and predicts future trends in nuclear medicine imaging technology. (Author/MM)

  8. Semen quality of young men from the general population in Baltic countries.

    Science.gov (United States)

    Erenpreiss, Juris; Punab, Margus; Zilaitiene, Birute; Hlevicka, Solveiga; Zayakin, Pawel; Matulevicius, Valentinas; Tomas Preiksa, Romualdas; Jørgensen, Niels

    2017-06-01

    What are the parameters of semen quality in Baltic men? Combined parameters of sperm concentration, motility and morphology revealed that 11-15% of men had low semen quality, 37-50% intermediate and 38-52% high semen quality. Previous studies have revealed regional differences in semen parameters, and semen quality of Baltic men has been suggested to be better than that of other European men. This was a cross-sectional study of 1165 men aged 16-29 years from Estonia (N = 573), Latvia (N = 278) and Lithuania (N = 314) conducted in 2003-2004. Men from the general population, median age 19.8 years, provided one semen sample each, had blood samples taken, had testis size determined, and provided information on lifestyle. Based on combined data of sperm concentration, sperm motility and morphology the cohort was classified into three categories: low, intermediate or high semen quality. Comparisons between groups (including subgroups of Estonian men of Russian versus Estonian ethnicity) were tested, adjusting for ejaculation abstinence and age. The median sperm concentration of the Estonian, Latvian and Lithuanian populations of Baltic men was 63 mill/ml. Low semen quality was detected in 11-15% of the men, intermediate in 37-50% and high in 38-52%. No crucial differences between national subgroups were detected, except that a higher percentage (9.6%) of the subgroup of Russian Estonians reported having had cryptorchidism compared to the other men (2.5-3.6%, P impact on both sperm concentration and total sperm counts (P 10 years ago. Thus, a recent change in semen quality cannot be excluded. Owing to the study design, it is assumed, but unproven, that the men were representative of the general populations. Some men were very young (16 years), however, this was also the case for other European studies of similar populations. Assessment of sperm motility is associated with inter-observer variation, and no quality control was undertaken for sperm motility assessment to

  9. Boar semen bacterial contamination in Italy and antibiotic efficacy in a modified extender

    Directory of Open Access Journals (Sweden)

    Carla Bresciani

    2014-02-01

    Full Text Available The aims of the study were to identify microbial flora in boar semen under field conditions in northern Italy, to investigate antibiotic resistance and sensitivity of isolated bacteria, and to evaluate elimination of bacteria after storage in two types of extenders added with different antibiotics (amikacin vs gentamicin. A total of 60 boars were collected in 13 pig farms. Bacteriological and mycological investigations were performed immediately on raw semen samples, then at 48 and 120 h of storage on semen diluted randomly in a new short-term modified extender (ME-S or in a commercial one (CRONOSTM. Bacterial contamination was found in 63% of raw semen samples and different bacterial species were isolated: E.coli, Serratia marcescens, Staphylococcus epidermidis and aureus, Proteus spp., Streptococcus spp. and Pseudomonas aeruginosa. E. coli was the most isolated contaminant (53%; Pseudomonas aeruginosa was found only in one semen sample. The analysis of variance of factors affecting contamination levels was significant for the farm of origin (P<0.05 and not significant for the breed. Antibiotic resistance of these bacteria was assessed using different antibiotics. Significant differences (P<0.05 between observed and expected frequencies of bacterial isolates resistant or not to the antibiotics contained in the extenders were found. At 48 h of storage a reduction of aerobic contamination was found after ME-S dilution by 85.3% and after CRONOSTM by 63.8%. This paper proved the presence of pathogenic bacteria in semen. We thus believe it is highly advisable to perform periodic microbiological screening of boar semen in the swine industry to avoid the use of low sperm quality.

  10. Effect of varicocelectomy on testis volume and semen parameters in adolescents: a meta-analysis

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    Tie Zhou

    2015-01-01

    Full Text Available Varicocele repair in adolescent remains controversial. Our aim is to identify and combine clinical trials results published thus far to ascertain the efficacy of varicocelectomy in improving testis volume and semen parameters compared with nontreatment control. A literature search was performed using Medline, Embase and Web of Science, which included results obtained from meta-analysis, randomized and nonrandomized controlled studies. The study population was adolescents with clinically palpable varicocele with or without the testicular asymmetry or abnormal semen parameters. Cases were allocated to treatment and observation groups, and testis volume or semen parameters were adopted as outcome measures. As a result, seven randomized controlled trials (RCTs and nonrandomized controlled trials studying bilateral testis volume or semen parameters in both treatment and observation groups were identified. Using a random effect model, mean difference of testis volume between the treatment group and the observation group was 2.9 ml (95% confidence interval [CI]: 0.6, 5.2; P< 0.05 for the varicocele side and 1.5 ml (95% CI: 0.3, 2.7; P< 0.05 for the healthy side. The random effect model analysis demonstrated that the mean difference of semen concentration, total semen motility, and normal morphology between the two groups was 13.7 × 10 6 ml−1 (95% CI: −1.4, 28.8; P = 0.075, 2.5% (95% CI: −3.6, 8.6; P= 0.424, and 2.9% (95% CI: −3.0, 8.7; P= 0.336 respectively. In conclusion, although varicocelectomy significantly improved bilateral testis volume in adolescents with varicocele compared with observation cases, semen parameters did not have any statistically significant difference between two groups. Well-planned, properly conducted RCTs are needed in order to confirm the above-mentioned conclusion further and to explore whether varicocele repair in adolescents could improve subsequently spontaneous pregnancy rates.

  11. Effects of pesticide use on semen quality among farmers in rural areas of Sabah, Malaysia.

    Science.gov (United States)

    Hossain, Feroz; Ali, Osman; D'Souza, Urban J A; Naing, Daw Khin Saw

    2010-01-01

    To determine the relationship between semen quality and exposure to pesticide residues. A cross-sectional study was conducted among male farmers from 3 different communities in Sabah, Malaysia. A total of 152 farmers participated in this study of whom 62 farmers had been exposed to either paraquat or malathion or both to varying extents. Questionnaires were designed to record a history of pesticides exposure and other potential risk factors among farmers. All semen samples were collected, processed and analyzed by qualified personnel based on WHO guidelines. Volume, pH, sperm concentration, motility, morphology and WBC count were examined and recorded. The association between pesticide exposure and semen parameters was highly significant. The mean values of volume, pH, sperm concentration, motility, and WBC count were significantly less in the exposed group than in compared with the non-exposed group, with p<0.005. Those who were exposed to pesticides had greater risk of having abnormal semen parameters than those in with the non exposed group, with p values of less than 0.05. The comparison between semen qualities such as lower sperm count, motility and higher percentage of sperm abnormality of those exposed to different types of pesticides (paraquat and malathion) showed no significant differences. The results showed a significant decline in semen quality with a decline in sperm count, motility and higher percent of teratospermia among subjects with pesticide exposure, and those who were exposed to pesticides had significantly 3 to 9 times greater risk of having abnormal semen parameters.

  12. Preservation of tomcat (Felis catus) semen in variable temperatures.

    Science.gov (United States)

    Siemieniuch, Marta; Dubiel, Andrzej

    2007-05-01

    The aim of our study was to estimate the viability of cat epididymal sperm in short time storage at +4 degrees C and in long term storage at -196 degrees C and to assess the percentage of live sperm in fresh semen using eosin/nigrosin staining compared to the flow cytometry method. The testes with epididymides were obtained after routine castration procedure. The sperm for further research were collected after flushing the epididymides using extender consist of: Tris 2.4 g, citric acid 1.4 g, glucose 0.8 g, 0.06% (w/v) Na-benzylpenicillin, 0.1% (w/v) streptomycin sulphate and distilled water. Half of each sample was equilibrated with the dilution and loaded in 0.25 ml plastic straws. The straws were placed on a rack in liquid nitrogen vapour at -120 degrees C for 10 min, plunged in liquid nitrogen for 10 min, replaced to marked goblets and loaded into canes for long term storage in liquid nitrogen at -196 degrees C. Sixty percent of motile spermatozoa was accomplished after thawing. However, the percentage of the sperm with intact acrosomes was decreased and the share of cells with midpiece and tail defects was increased. The storage of sperm flushed from epididymides at +4 degrees C for a short time and the usage of sperm during 2-3 days after collection seems to be better than cryopreservation. In our study, normospermia was present in 72.7 +/- 8.8% of fresh semen. The most common defect was the presence of distal droplets, imperfect heads or abnormal acrosomal outline. The motility of fresh sperm flushed from epididymides achieved 77.9 +/- 6.8%. The viability of sperm amounting to 52.5 +/- 13.8% was achieved on third day of conservation in the liquid extender. The percentage of viable sperm in fresh epididymal spermatozoa was 84.9 +/- 7.8%. Compared to these results, the percentage of live cells using SYBR-14/propidium iodide staining was insignificantly lower (82.2 +/- 8%). The live, non-apoptotic cells were 79.0 +/- 7.8%. The share of live, early

  13. Effective freezing rate for semen cryopreservation in endangered Mediterranean brown trout (Salmo trutta macrostigma) inhabiting the Biferno river (South Italy).

    Science.gov (United States)

    Iaffaldano, Nicolaia; Di Iorio, Michele; Manchisi, Angelo; Esposito, Stefano; Gibertoni, Pier Paolo

    2016-10-01

    This study was designed to determine: (i) the in vitro effects of different freezing rates on post-thaw semen quality of Mediterranean brown trout (Salmo trutta macrostigma) from the Biferno river; and (ii) the in vivo fertilization and hatching percentage of freezing rate giving rise to the best post-thaw semen quality. Pooled semen samples were diluted 1:3 (v:v) in a freezing extender composed of 300 mM glucose, 10% egg yolk and 10% dimethyl sulfoxide (DMSO). The extended semen was packaged in 0.25 ml plastic straws and frozen at different heights above the liquid nitrogen surface (1, 5 or 10 cm) for 10 min to give three different freezing rates. Semen samples were thawed at 30°C for 10 s. The variables assessed after thawing were sperm motility, duration of motility and viability. Our results clearly indicate a significant effect of freezing rate on post-thaw semen quality. Semen frozen 5 cm above the liquid nitrogen surface showed the best quality after freezing/thawing. Based on these in vitro data, 2 groups of 200 eggs were fertilized with fresh semen or semen frozen 5 cm above the liquid nitrogen surface. Fertilization and hatching rates recorded for eggs fertilized with frozen semen were significantly lower (25.4% and 22.5%, respectively) than the ones obtained using fresh semen (87.8% and 75.5%, respectively). An effective freezing protocol will allow for the creation of a sperm cryobank to recover the original population of Mediterranean brown trout in the Biferno river.

  14. Fertilitas Semen Kerbau Rawa (Bubalus bubalis carabanensis yang Diencerkan dengan Pengencer Nira Aren (FERTILITY OF SWAMP BUFFALO SEMEN (BUBALUS BUBALIS CARABANENSIS DILUTED WITH SUGAR PALM JUICE EXTENDER

    Directory of Open Access Journals (Sweden)

    Muhammad Rizal

    2016-11-01

    Full Text Available Sugar palm juice (Arenga pinnata Merr. can be used as semen extender because its various nutrientscontent which were needed for spermatozoa preservation. The objective of this research was to examineeffectivity of sugar palm juice as swamp buffalo semen extender in artificial insemination (AI program.Semen of swamp buffalo were collected by using an artificial vagina. Fresh semen were evaluated anddivided in equal volume into four tubes and diluted with lactose extender containing 20% egg yolk (lactoseor control, 85% sugar palm juice + 15% egg yolk (PJEY15, 80% sugar palm juice + 20% egg yolk (PJEY20,and 75% sugar palm juice + 25% egg yolk (PJEY25, respectively. Diluted-semen were preserved inrefrigerator at 5oC, and quality of spermatozoa including percentages of motile spermatozoa, livespermatozoa, and intact plasma membrane (IPM were evaluated every day for four days. A total of 13female swamp buffaloes were estrous synchronize with PGF2a, and six buffaloes were inseminated withlactose and seven buffaloes were inseminated with PJEY20. Results of this research showed that at dayfourpreservation, mean percentages of motile and live spermatozoa for lactose (36.67 and 51.83% andPJEY20 (35.83 and 51.5% were significantly (P<0.05 higher than PJEY15 (31.67 and 44% and PJEY25 (30 and 45.33%, but no difference significant between treatment for IPM parameter. Pregnancy rate forlactose was 66.67% and 71.43% for PJEY20. Percentage of birth for lactose and PJEY20 were 66.67% and71.43%, respectively. In conclusion, sugar palm juice could be used as an alternative extender in semenpreservation process and AI of swamp buffalo.

  15. Semen cryopreservation in fish: effects on sperm motility and fertility

    International Nuclear Information System (INIS)

    Martinez, Jose Gregorio; Pardo Carrasco, Sandra

    2010-01-01

    The cryopreservation of semen in fish, as in many species even shows effects that decrease sperm quality and directly engage cell ability to successfully participate in the processes of fertilization and embryonic development. the characteristics such as mobility and fertilizing capacity of fertilization of sperm are considered to be quality criteria that allow to measure the success or failure of the process, since they are considered integrative variables, being indicators that depend not on a single factor, but on the stability and welfare of all structures, enzymes and subcellular functional compounds that give place to these spermatic characteristics. membrane damage (Adenylate cyclase, ion channels, grouping of other proteins, among others) and their implication in the route of signaling pathway leading to spermatic activation, ATP degradation and fragmentation of nuclear and mitochondrial DNA (genome), degradation of kinase enzymes and other cytosolic proteins (proteome) are considered today, as some of the molecular factors that most affect during cryopreservation and markedly decreasing the fertilizing capacity and mobility of sperm in fish. Proposals on the molecular mechanisms, by which these subcellular factors interact and act as consequence of cryopreservation, are some of the topics covered in this review. Understanding the principles and factors that are involved in the origin of such damages, will allow to improved cryopreservation processes, making them less harmful and more efficient.

  16. Altered expression of KLC3 may affect semen parameters

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    Pegah Kargar- Dastjerdy

    2016-01-01

    Full Text Available Background: KLC3 protein as a member of the kinesin light-chain protein family plays an important role in spermatogenesis, during formation of mitochondrial sheath in the mid piece of the sperm tail. Objective: This study for the first time aims to compare the expression of the KLC3 gene between fertile and infertile individuals. Materials and Methods: Semen samples were collected from 19 fertile individuals who were selected from embryo-donor volunteers and 57 infertile individuals who had abnormal sperm parameters according to world health organization criteria. Sperm parameters using computer assisted sperm analysis and the quantitative KLC3-gene expression using the real-time PCR method were measured. Results: Our results revealed a significant correlations between sperm concentration with relative expression of KLC3 only in infertile groups (r=0.45, p=0.00. A significant correlation was not found between KLC3 expression and sperm motility; however, the relative expression of KLC3 was significantly higher in asthenozoospermic compared to non-asthenozoospermic individuals. Conclusion: Low expression of KLC3 may result in improper function of midpiece, which has important function in sperm motility. The results of this study show that aberrant expression of KLC3 might be associated with phenomena like oligozoospermia and asthenozoospermia. This article is extracted from student’s thesis.

  17. Artificial insemination field data on the use of sexed and conventional semen in nulliparous Holstein heifers.

    Science.gov (United States)

    Healy, A A; House, J K; Thomson, P C

    2013-03-01

    This study investigated conception rates and other reproductive outcomes achieved with artificial insemination (AI) of nulliparous Holstein heifers using sexed and conventional semen in a commercial Australian dairy herd in central western New South Wales from January 2004 to April 2009. Retrospective data from on-farm records of 9,870 inseminations of 4,456 heifers were analyzed using several mixed models to assess the effect of temperature and humidity surrounding breeding, insemination sire, artificial insemination technician, service number, and heifer weight and age at breeding on reproductive traits (conception rates, sex ratios, gestation length, and abortion and stillbirth rates). Semen was used from 15 sexed sires and 41 unsexed sires. Sexed semen was primarily used at first and second service. Empirical conception rates of 31.6 and 39.6% were achieved for sexed and unsexed semen respectively, whereas model-based predictions were lower, at 21.3 and 32.1%. Conception rates were significantly affected by insemination sire, sex-sorting, heifer age at breeding, temperature and humidity surrounding breeding, service number, and AI technician. Sexed semen yielded 86% heifers, compared with 48% for conventional semen. Significant predictors of calf sex included semen sexing, gestation length, and insemination sire. Twinning rate was high, at 3.6% for both semen types, and gestation length and heifer weight at breeding were significant predictors of twinning. Abortion rates for sexed and unsexed conceptions were similar at 6.1 and 6.5%, respectively, and were affected by heifer age at breeding. Stillbirth rate was affected by calf sex, twinning, gestation length, and AI technician; semen sorting, age at breeding, and temperature and humidity were marginally significant predictors. No abnormalities were observed in the development of offspring, except for a marginally higher stillbirth rate for sexed calves, a finding that needs further investigation. Many

  18. Simple and effective methods of freezing capercaillie (Tetrao urogallus L. semen.

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    Artur Kowalczyk

    Full Text Available A continuous decline in the number and range of capercaillie (Tetrao urogallus L. in many European countries can be observed, mostly due to habitat destruction by human activity, unecological forestry management, and increased density of natural predators. Ex situ in vitro gene banks provide a unique opportunity to preserve the genetic material for future generations. Simple and effective cryopreservation methods for capercaillie semen are discussed. Semen was collected from seven males kept in the Capercaillie Breeding Centre at Forestry Wisła in Poland. Within five minutes after collection, ejaculates were diluted with EK diluent, then divided into two parts, and subjected to two freezing procedures: in pellets and in straws. In fresh semen, ejaculate clearness, viscosity, color and volume, as well as sperm concentration, motility and morphology, were evaluated, while in frozen-thawed semen only motility and morphology of sperm were determined. Fertilizing ability of thawed semen was examined for samples frozen in straws. Significant (P<0.05 differences between individual males were found in relation to the majority of fresh semen traits: ejaculate volume averaged 102.1 µL (varying from 49.0 to 205.0; average sperm concentration was 632.5 x 10⁶ mL⁻¹ (178.8-1257.1; percentage of live normal cells varied from 39.2 to 70.3% (58.7% on an average; percentage of motile cells ranged from 76.0 to 85.7% and motility parameters were male dependent, as well. Both cryopreservation methods had a negative effect on morphology and motility of frozen-thawed semen; however, the straw method yielded 60.7% and the pellet method 42.5% of live cells in total in thawed semen (P<0.05, while the number of live normal (intact cells was similar (22.4 and 22.2%, respectively. Egg fertility varied between 77.8 and 91.7% (average 84.4%. Both freezing procedures seem to be effective in obtaining acceptable viability and high fertilizing potency of thawed sperm and

  19. Study of correlation between reaction time and refractory period (as indices of libido) with semen characteristics in Arkharmerino×Moghani and Baluchi×Moghani rams

    OpenAIRE

    Ali A.R. Rahimi; Gholamali Moghaddam; Mohamad M. Pourseif

    2012-01-01

    This study was conducted on 10 crossbred rams consisted of 5 ArkharMerino×Moghani (AM×MG) and 5 Baluchi×Moghani (BL×MG), to analyze correlation between semen characteristics and libido activity. The semen samples were evaluated for semen volume, total sperm per ejaculate (TSE), spermatozoa concentration (SC), color, wave motion, spermatozoa progressive motility, percentage of live and abnormal spermatozoa, pH, rate of metabolic activity of spermatozoa and semen index. The libido of rams was m...

  20. Semen quality and the level of reproductive hormones after environmental exposure to pyrethroids.

    Science.gov (United States)

    Radwan, Michał; Jurewicz, Joanna; Wielgomas, Bartosz; Sobala, Wojciech; Piskunowicz, Marta; Radwan, Paweł; Hanke, Wojciech

    2014-11-01

    The aim of this study was to determine whether the environmental exposure to pyrethroids affects semen quality and the level of reproductive hormones in men. The study population consisted of 334 men who attended the infertility clinic for diagnostic purposes and who had normal semen concentration of 15 to 300 mln/mL. Participants were interviewed and provided a semen sample. The pyrethroids metabolites-3-phenoxybenzoic acid (3PBA), cis-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylic acid (CDCCA), trans-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylic acid (TDCCA), and cis-2,2-dibromovinyl-2,2-dimethylcyclopropane-1-carboxylic acid (DBCA) were analyzed in the urine using a validated gas chromatography ion-trap mass spectrometry method. Urinary pyrethroids metabolites levels were significantly associated with an increase in the percentage of sperm with abnormal morphology and decrease in sperm concentration, the level of testosterone, and computer-aided semen analysis parameters. Environmental pyrethroids exposure may affect semen quality and the level of reproductive hormones.

  1. EFFECT OF ANTIBIOTICS IN EXTENDER ON FERTILITY OF LIQUID BUFFALO BULL SEMEN

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    S. AKHTER, M. SAJJAD, S. M. H. ANDRABI1, N. ULLAH1 AND M. QAYYUM

    2007-01-01

    Full Text Available This study was carried out to determine if a new antibiotic combination comprising of gentamycin, tylosin and linco-spectin (GTLS in extender is suitable for improvement in fertility of liquid buffalo bull semen through artificial insemination (AI. Two consecutive ejaculates per week (4 weeks were collected from three Nili-Ravi buffalo bulls of known fertility by using artificial vagina. The pooled ejaculates were split-sampled and diluted with skimmed milk extender (37oC; 10x106 motile spermatozoa/ml containing either SP (streptomycin 1000 μg/ml and penicillin 1000 iu/ml or GTLS (gentamycin 500 μg/ml, tylosin 100 μg/ml, lincomycin 300 μg/ml, and spectinomycin 600 μg/ml. Liquid semen was stored at 5°C for seven days. Fertility, based on 90-days first service pregnancy rate, was determined under field conditions. The fertility rates for SP-based vs. GTLS-containing liquid semen of buffalo bull were 58.55 and 60.00%, respectively, the difference was non significant. The fertility rates also did not differ (P>0.05 due to antibiotics at different days of storage of liquid semen at 5oC. In conclusion, GTLS, in skimmed milk extender compared to SP, did not significantly improve the fertility of chilled buffalo bull semen.

  2. Assessment of semen quality in Swamp Buffalo AI Bulls in Thailand

    Directory of Open Access Journals (Sweden)

    S. Koonjaenak

    2010-02-01

    Full Text Available Characteristic of Thai swamp buffalo bulls semen used for artificial insemination (AI in Thailand, aspects relevance in freezing and thawing of semen are review. Semen and sperm characteristics were evaluated included sperm count, motility (assessed subjectively and by CASA, morphology (using phase-contrast light microscopy and SEM, plasma membrane integrity (PMI (using a hypo-osmotic swelling test [HOST] and SYBR- 14/propidium iodide [PI], plasma membrane stability (PMS (using Annexin-V/PI and deoxyribonucleic acid (DNA integrity (using SCSA and flow cytometry [FCM]. The average ejaculate volume was about 3.0–4.0 mL, with good viability (PMI measured by the HOST and motility (>65% and >70%, respectively. Sperm concentration ranged from 1.1 to 1.2 billion/mL, being also affected by bull age. Whereas semen quality (including sperm output, pH and initial sperm motility did not differ between the seasons. Few spermatozoa (<15%/ ejaculate had abnormal morphology with abnormalities resembling those in other bovidae. In FT semen, PMI (using SYBR-14/PI and PMS were highest in winter. Across seasons, ~50% of post-thaw spermatozoa depicted linear motility, a proportion that decreased to ~35% during incubation (38oC for 60 minutes, without marking any seasonal difference. The sperm DNA was hardly damaged (with <3% fragmentation, expressed as DNA fragmentation index [DFI], among seasons.

  3. Microsatellite analysis of cryopreserved stallion semen stored on FTA(R paper : research communication

    Directory of Open Access Journals (Sweden)

    M.L. Schulman

    2002-07-01

    Full Text Available The aim of this study was to establish and validate a method to permit microsatellite analysis of DNA profiles obtained from frozen-thawed stallion sperm cells. This would provide reliable and accurate verification of the identification of a semen donor. Ejaculates from 5 pony stallions were collected, processed and frozen in 0.5 m plastic straws. Aliquots of 100 m of the frozen-thawed semen thus obtained were either placed directly, or diluted (1 : 10 ; 1 : 100 ; and 1 : 1000 and placed on slides of FTA(R paper. Similarly, blood samples obtained from each of the stallions were placed onto slides of FTA(R paper. A punch was removed from each sample after drying. Each sample was mixed with FTA(R purification reagent, Dithiothreitol and Proteinase K before incubation and processing. All samples were processed with a set of 13 microsatellite markers. Further analysis permitted a comparison of the DNA profiles of the frozen-thawed semen and the blood samples. A full profile of markers was obtained from the 1 : 10 and 1 : 100 dilutions of the frozen-thawed semen samples as well as from the blood samples. The DNA profiles from the frozen-thawed semen and blood samples obtained from the stallions matched in all cases.

  4. [Comparison of reactive oxygen species production in neat semen and washed spermatozoa].

    Science.gov (United States)

    Svobodová, M; Oborná, I; Fingerová, H; Novotný, J; Brezinová, J; Radová, L; Vyslouzilová, J; Horáková, J; Grohmannová, J

    2009-12-01

    To determine Reactive Oxygen Species (ROS) production in neat semen and spermatozoa suspension using chemiluminescence and to examine correlation between both methods. Prospective laboratory study. Department of Obstetric and Gynecology, University Hospital, Olomouc. The study included fertile volunteers (FV, n = 17), men from infertile couples (NM, n = 19) and men with idiopathic infertility (NMI, n = 15). ROS levels were determined by the same method in neat and washed semen samples. The ROS production in neat semen was lower than that in spermatozoa suspension. There was no significant diference in ROS production between volunteers and males from infertile couples. There was a significant correlation between log ROS in neat semen and in spermatozoa suspension in studied groups (FV r = 0.85, p = 1.5 x 10(-5); NM r = 0.76, p neat semen is simpler, faster and better reflecting the actual level of oxidative stress than the same measurement in spermatozoa suspension. The implementation of this method can complement the algorithm of diagnostics and treatment of male infertility and be helpful in selection of patients for antioxidant or antibiotic treatment.

  5. Zinc and iron concentration and SOD activity in human semen and seminal plasma.

    Science.gov (United States)

    Marzec-Wróblewska, Urszula; Kamiński, Piotr; Lakota, Paweł; Szymański, Marek; Wasilow, Karolina; Ludwikowski, Grzegorz; Kuligowska-Prusińska, Magdalena; Odrowąż-Sypniewska, Grażyna; Stuczyński, Tomasz; Michałkiewicz, Jacek

    2011-10-01

    The aim of the present study was to measure zinc (Zn) and iron (Fe) concentration in human semen and superoxide dismutase (SOD) activity in seminal plasma and correlate the results with sperm quality. Semen samples were obtained from men (N = 168) undergoing routine infertility evaluation. The study design included two groups based on the ejaculate parameters. Group I (n = 39) consisted of males with normal ejaculate (normozoospermia), and group II (n = 129) consisted of males with pathological spermiogram. Seminal Zn and Fe were measured in 162 samples (group I, n = 38; group II, n = 124) and SOD activity in 149 samples (group I, n = 37; group II, n = 112). Correlations were found between SOD activity and Fe and Zn concentration, and between Fe and Zn concentration. SOD activity was negatively associated with volume of semen and positively associated with rapid progressive motility, nonprogressive motility, and concentration. Negative correlation was stated between Fe concentration and normal morphology. Mean SOD activity in seminal plasma of semen from men of group I was higher than in seminal plasma of semen from men of group II. Fe concentration was higher in teratozoospermic males than in males with normal morphology of spermatozoa in group II. Our results suggest that Fe may influence spermatozoa morphology.

  6. An automated smartphone-based diagnostic assay for point-of-care semen analysis

    Science.gov (United States)

    Kanakasabapathy, Manoj Kumar; Sadasivam, Magesh; Singh, Anupriya; Preston, Collin; Thirumalaraju, Prudhvi; Venkataraman, Maanasa; Bormann, Charles L.; Draz, Mohamed Shehata; Petrozza, John C.; Shafiee, Hadi

    2017-01-01

    Male infertility affects up to 12% of the world’s male population and is linked to various environmental and medical conditions. Manual microscope-based testing and computer-assisted semen analysis (CASA) are the current standard methods to diagnose male infertility; however, these methods are labor-intensive, expensive, and laboratory-based. Cultural and socially dominated stigma against male infertility testing hinders a large number of men from getting tested for infertility, especially in resource-limited African countries. We describe the development and clinical testing of an automated smartphone-based semen analyzer designed for quantitative measurement of sperm concentration and motility for point-of-care male infertility screening. Using a total of 350 clinical semen specimens at a fertility clinic, we have shown that our assay can analyze an unwashed, unprocessed liquefied semen sample with smartphone capabilities, can make remote semen quality testing accessible to people in both developed and developing countries who have access to smartphones. PMID:28330865

  7. Cryopreservation of cattle semen using coconut water extender with different glycerol concentrations

    Directory of Open Access Journals (Sweden)

    Reda Ibrahim El-Sheshtawy

    2017-11-01

    Full Text Available Objective: To investigate the effect of coconut water with a lone concentration and different concentrations of glycerol on chilled and cryopreserved cattle semen characteristics. Methods: Semen was collected from five mature cattle bulls, at weekly intervals for 5 weeks. The ejaculates were pooled and evaluated for dilution processing. Tris citrate egg yolk fructose was used as control treatment for semen, while 50% (V/V coconut water, 25% (V/V bidistilled water and 25% (V/V, 5% anhydrous monosodium citrate to 20 mL egg yolk and three different concentrations of glycerol (4%, 6% and 8% were used as coconut water (CW- glycerol-yolk extenders (CWCG-4, CWCG-6 and CWCG-8. Extended semen was cooled and cryopreserved. Sperm motility%, sperm membrane integrity%, normal acrosome%, live sperm% and total sperm abnormalities% were recorded after equilibrium and after freezethawing. Results: The addition of 4% glycerol to coconut water enriched media (CWCG-4 revealed the most effective addition of glycerol on all parameters after equilibrium and after freeze-thawing. Conclusions: Coconut water enriched media with 4% glycerol addition is safe to be used as an extender in bull semen preservation because it is a sterile liquid. So, it can be used without addition of antibiotics to the extender, as antibiotics have to some extent hazardous effect on spermatozoa.

  8. Use of commercial extenders and alternatives to prevent sperm agglutination for cryopreservation of brown bear semen.

    Science.gov (United States)

    Gomes-Alves, S; Alvarez, M; Nicolas, M; Lopez-Urueña, E; Martínez-Rodríguez, C; Borragan, S; de Paz, P; Anel, L

    2014-08-01

    The objective of this study was to evaluate different bovine and canine commercial semen extenders for cryopreservation of brown bear ejaculates and the effect of semen collection directly into extender on sperm agglutination. Semen samples were obtained by electroejaculation from 13 adult males. In experiment 1, eleven ejaculates from eight bears were used to evaluate Bioxcell and Andromed as extenders, whereas in experiment 2, nine ejaculates from six bears were used to evaluate Triladyl canine, CaniPro, and Extender 2 as extenders. An extender specifically developed for brown bears (Test-Tris-fructose-egg yolk-glycerol, TTF-ULE/bear) served as a control extender in both experiments. After thawing, total and progressive sperm motility and sperm viability were greater (P bear and Andromed extenders than for Bioxcell in experiment 1 and greater (P bear extender than for Triladyl Canine, CaniPro, and Extender 2 in experiment 2. In experiment 3, addition of handling extender (TTF-H) to the semen collection tube for eight ejaculates from seven bears resulted in less (P bear is the most suitable extender for brown bear semen cryopreservation, but comparable results can be obtained with the commercial extender Andromed. In addition, collection of ejaculates directly in TTF-H extender decreases sperm agglutination in fresh samples. Copyright © 2014 Elsevier Inc. All rights reserved.

  9. Analisis Pemilihan Pemasok dengan Metode Analitycal Hierarchy Process di Proyek Indarung VI PT Semen Padang

    Directory of Open Access Journals (Sweden)

    Suci Oktri Viarani

    2016-04-01

    Full Text Available The cement industry is an industry that is desperately needed by the public. Cement always used to make houses, buildings, bridges and other buildings. Heightened demand for cement, making PT Semen Padang, which is one of the largest producers of cement in Indonesia, wants to increase the productivity of cement annually. One way is to set up a new cement plant that is able to meet consumer demand. The establishment of a new plant would require careful planning, especially planning development and planning costs. To set up a new plant, also required the planning of goods and services to be used in its manufacture. Therefore, it is necessary a good supplier that is able to provide the needs according to required specifications. The Indarung Project VI, PT Semen Padang requires the right supplier selection method, so as to provide quality goods and services. The method of procurement of goods and services performed Indarung Project VI, PT Semen Padang today is the commonly used method of procurement. AHP (Analytical Hierarchy Process is a systematic method and does not require a long time, and can show priority weights of criteria and selected suppliers. This study using AHP to select suppliers of goods and services in the Indarung Project VI, PT Semen Padang. Based on supplier selection has been made, PT ABB Industrial Way elected as a supplier to supply substations for the Indarung Project VI, PT Semen Padang.

  10. The use of maca (Lepidium meyenii) to improve semen quality: A systematic review.

    Science.gov (United States)

    Lee, Myeong Soo; Lee, Hye Won; You, Sooseong; Ha, Ki-Tae

    2016-10-01

    The aim of this review was to assess the evidence for the effectiveness of maca (Lepidium meyenii) in improving semen quality. We searched 11 databases from their inception to March 2016 and included all clinical trials on the improvement of semen quality parameters in infertile and healthy men, regardless of the study design or the type of maca. The risk of bias for each study was assessed using the Cochrane criteria. The selection of studies, data extraction, and validation were performed independently by the first two authors. Discrepancies were resolved through discussion by the same two authors. Five studies - 3 randomized clinical trials (RCTs) and 2 uncontrolled observational studies (UOSs) - met all of the inclusion criteria. One RCT found favorable effects of maca on sperm mobility in infertile men. The two other RCTs showed positive effects of maca on several semen quality parameters in healthy men. The two UOSs also suggested favorable effects of maca on semen quality. The results of our systematic review provide suggestive evidence for the effectiveness of maca in improving semen quality. However, the total number of trials, the total sample size, and the risk of bias of the included studies prevent the drawing firm conclusions. More rigorous studies are warranted. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  11. Effect of sugars on characteristics of Boer goat semen after cryopreservation.

    Science.gov (United States)

    Naing, S W; Wahid, H; Mohd Azam, K; Rosnina, Y; Zuki, A B; Kazhal, S; Bukar, M M; Thein, M; Kyaw, T; San, M M

    2010-10-01

    In order to improve Boer goat semen quality during cryopreservation process, the influence of sugar supplementation on semen characteristics of sperm were investigated. Three experiments were carried out to investigate the effect of (a) addition of two monosaccharides (fructose and glucose) and two disaccharides sugars (trehalose and sucrose) (b) sugar combination (fructose and trehalose, sucrose and trehalose, glucose and trehalose), and control (glucose without trehalose) (c) different concentrations of trehalose on cryopreservation using Tris based extender. The total motility, forward motility, viability, normal spermatozoa, acrosome integrity and membrane integrity were assessed subjectively. Differences were not detected among monosaccharides, but glucose increased (Pdiffer (P>0.05) among disaccharide sugar supplementation. Combination of glucose and trehalose significantly improved the characteristics of Boer spermatozoa after cryopreservation (P<0.05). Supplementation of trehalose (198.24mM) into the glucose extender significantly increased total motility, forward motility, live spermatozoa, acrosome integrity and membrane integrity following cryopreservation (P<0.05). In conclusion, glucose had the better ability to support Boer sperm motility and movement patterns. Combination of monosaccharide (glucose) and disaccharide (trehalose) improved semen quality following cryopreservation. Trehalose supplementation at the concentration of 198.24mM to the glucose extender conferred the greater improvement of semen quality for Boer semen cryopreservation. Copyright © 2010 Elsevier B.V. All rights reserved.

  12. EFFECT OF DIFFERENT CRYOPROTECTIVE AGENTS ON SKIM MILK AND DIMITROPOULUS EXTENDER FOR STALLION SEMEN CRYOPRESERVATION

    Directory of Open Access Journals (Sweden)

    R.I. Arifiantini

    2014-10-01

    Full Text Available s to assess different CPAs on stallion semen cryopreservation. Skim milk (SM and Dimitropoulos(DV were the extenders used in this study; each was added by glycerol (Gly, combination of ethyleneglycol-glycerol (EG+Gly or dimethilformamide (DMF. Each semen sample was evaluated and dividedequally into six tubes; semen in the three tubes was diluted 1:1 with (SM, while in the remaining tubesthe semen was diluted 1:1 by DV. After being diluted, all tubes were centrifuged at 1006xg for 10minutes. The supernatan discarded, the pellet was rediluted by SM trehalosa or DV trehalose, and addedby G, EG+Gly, or DMF to reach the final sperm concentration of 200x106/ml. The extended semen wasindividually packed in 0.3 ml minitube, equilibrated at 4oC for 2 hours, frozen in liquid nitrogen vaporfor 10 minutes, and then was stored in liquid nitrogen container at -196 oC. After 24 hours, the semenwas thawed at 37 oC for 30 second. There were no significantly different (p>0.05 on the percentages ofmotile and viable sperm in SMT (21.7% and 43.4%, respectively compared with those extended withDV T extender (26.9% and 50.8%, respectively. DMF demonstrated better results as CPA compared tothe others; and DVTDMF combination had the best protection during cryopreservation in this study.

  13. Pattern of semen fluid abnormalities in male partners of infertile couples in southeastern, Nigeria.

    Science.gov (United States)

    Ugboaja, J O; Monago, E N; Obiechina, N J A

    2010-01-01

    The incidence of male infertility is increasing in our environment. There is a need to evaluate the pattern of abnormality with a view to recommending appropriate interventions. We aimed to to analyze the seminal fluid parameters of the male partners of the infertile couples managed in the hospital over a 12 month period and to identify the pattern of abnormalities. A retrospective study of all the semen samples of male partners of infertile couples submitted for analysis to the microbiology laboratory of Nnamdi Azikiwe University Teaching Hospital, Nnewi Nigeria between 1st January 2006 and 31st December 2006 The reports of the semen fluid analysis were retrieved from the records department and supplemented with the laboratory register. Out of the 348 semen sample reports evaluated, 237 (68.0%) had semen fluid abnormalities. 104 (30.0%) had single factor abnormalities while 133 (38.0%) had combined factor anomalies. Asthenozoospermia 58 (16.7%) was the main single abnormality, while Astheno-oligozoospermia 51 (14.7%) and Astheno-oligoteratozoospermia (13.2%) were the major combined factor abnormalities detected. Very few 5 (1.4%) of the patients had azospermia. The study showed a high rate of semen fluid abnormalities among the male partners of infertile women in our environment. The high preponderance of poor motility emphasizes the need to include men in programmes aimed at reducing sexually transmitted infections in Nigeria.

  14. Hematologic Profile and Semen Quality of Male Timor Deer (Rusa timorensis) at Various Hierarchies

    Science.gov (United States)

    Samsudewa, D.; Capitan, S. S.; Sevilla, C. C.; Vega, R. S. A.; Ocampo, P. P.

    2018-02-01

    The aim of this research was to observe hematologic profile i.e. erythrocyte count, hemoglobin and hematocrit and semen quality, i.e. semen volume, sperm motility and sperm abnormality of α-male, β-male and subordinate male Timor deer raised under captivity. Twelve males (51 ± 6 months old; 68.29 ± 8.41kg body weight) at similar antler stages were use in this study. Before and after 43 days of establishment of dominance hierarchy blood were sampled after sedation for erythrocyte count, hemoglobin (mg/dL), and hematocrit (%). Likewise, semen was collected using electroejaculator and were analyzed for semen volume (ml), sperm motility (%) and sperm abnormality (%) to compare male deer at various heirarchies. Wilcoxon signed ranks test and Kruskal-Wallis H test of non-parametric analysis was done. Significant difference was tested with Mann-Whitney U test. The results showed that highest count of erythrocyte shown on α and β-male (1.60 million per µL). The highest increase in hematocrit was observed in β-male (5%) and then followed by S2-male (4%). S2-male had the highest increase in hemoglobin (0.13 g/dL). The highest increase in semen volume was observed in α -male (0.75 ml). Social stress affected negatively the sperm motility and abnormality (P<0.05). The highest decrease was observed in S2-male.

  15. Use of frozen-thawed semen in equine artificial insemination programmes

    Directory of Open Access Journals (Sweden)

    Alonso A

    2016-12-01

    Full Text Available The use of equine frozen semen has variable results. Pregnancy rates per cycle may be between 10 to 70%. Although an inseminating dose of 280 a 320 millones progressive motile sperm (PMS has been recommended, under experimental conditions it is posible to obtain acceptable results with doses of 50 to 100 millones PMS. Deep intrauterine insemination is prefered when using frozen thawed semen, which consists in depositing the dose at the tip of the horn, ipsiltateral to ovulation. Young mares usually have better uterine condition and that increase their chance of become pregnant. It is important to make a correct follicular monitoring, in order to determine the right time to induce ovulation. Ovulation may be induced with Human Chorionic Gonadotrophin (hCG or deslorelin. It posible to perform a fixed time insemination or to choose an ultrasound follicular following protocol, inseminating within 6 h of ovulation. This option offers the best prenancy rates. Our laboratory manages a protocol which includes ultrasound monitoring every 6 hours, beginning 24 hous after induction of ovulation; this protocol had a first insemination pregnancy rate of 73%. If we consider that one of the main disadvantages of frozen-thawed semen is decreased fertility, these results are encouraging, since they are similar to those obtained with fresh or cooled semen, so it may represent an advantage when having stored semen of stallions that are dead, or have been sold or even castrated.

  16. Nuclear medicine

    International Nuclear Information System (INIS)

    Lentle, B.C.

    1986-01-01

    Several growth areas for nuclear medicine were defined. Among them were: cardiac nuclear medicine, neuro-psychiatric nuclear medicine, and cancer diagnosis through direct tumor imaging. A powerful new tool, Positron Emission Tomography (PET) was lauded as the impetus for new developments in nuclear medicine. The political environment (funding, degree of autonomy) was discussed, as were the economic and scientific environments

  17. Heart failure - medicines

    Science.gov (United States)

    CHF - medicines; Congestive heart failure - medicines; Cardiomyopathy - medicines; HF - medicines ... will need to take most of your heart failure medicines every day. Some medicines are taken once ...

  18. Does weight loss improve semen quality and reproductive hormones? Results from a cohort of severely obese men

    DEFF Research Database (Denmark)

    Håkonsen, Linn Berger; Thulstrup, Ane Marie; Aggerholm, Anette Skærbech

    2011-01-01

    A high body mass index (BMI) has been associated with reduced semen quality and male subfecundity, but no studies following obese men losing weight have yet been published. We examined semen quality and reproductive hormones among morbidly obese men and studied if weight loss improved...

  19. Excretion of bovine herpesvirus 1 in semen is detected much longer by PCR than by virus isolation

    NARCIS (Netherlands)

    Engelenburg, van F.A.C.; Schie, van F.W.; Rijsewijk, F.A.M.; Oirschot, van J.T.

    1995-01-01

    To compare the sensitivities of PCR and virus isolation and to examine the course of virus excretion in semen, we intrapreputially inoculated eight bulls with bovine herpesvirus 1 (BHV1) and used two bulls as sentinels. From these bulls, we collected a large panel of semen samples during 65 days

  20. Environmental pollution due to cadmium: measure of semen quality as a marker of exposure and correlation with reproductive potential.

    Science.gov (United States)

    De Franciscis, P; Ianniello, R; Labriola, D; Ambrosio, D; Vagnetti, P; Mainini, G; Trotta, C; Mele, D; Campitiello, M R; Caprio, F

    2015-01-01

    Contradictory reports exist in the literature regarding an association of cadmium with parameters of semen quality. The aim of the study was to assess cadmium levels in both blood and seminal plasma and to analyze the relationships between cadmium concentrations and lifestyle and semen parameters. Fifty healthy male volunteers were recruited to provide semen and blood samples. Each patient completed an extensive questionnaire regarding his occupation, residence, social status, diet, water source, smoking habits, and medical and surgical history. Semen analysis was carried out according to WHO guidelines. Detection of cadmium in both semen and blood samples was carried out by means of atomic absorption spectrophotometer. Mean concentrations of cadmium were 8.18 ± 1.6 ng/ml in blood samples and 2.56 ± 0.9 ng/ml in semen samples. Cadmium blood levels were significantly higher in men from industrialized areas and in current smokers, but were not correlated with semen levels. A significant positive correlation was found between cadmium blood levels, number of immotile spermatozoa, and teratozoospermia index (TZI). Significant inverse relationships between cadmium blood concentration and type-a and type a + b motility were found. The present data show a significant correlation between blood cadmium concentrations, cigarette smoking, occupational exposure, and parameters of semen quality. Such a reduction in spermiogenetic function could be an early marker of a toxic effect by cadmium pollution.

  1. Cryopreservation of turkey semen: effects of breeding line and freezing method on post-thaw sperm quality, fertilization, and hatching

    NARCIS (Netherlands)

    Long, J.A.; Purdy, P.H.; Zuidberg, C.A.; Hiemstra, S.J.; Velleman, S.G.; Woelders, H.

    2014-01-01

    Cryopreservation methods for poultry semen are not reliable for germplasm preservation, especially for turkeys, where fertility rates from frozen/thawed semen are particularly low. The objective was to evaluate cryopreservation methods for effectiveness in promoting cryosurvival and post-thaw

  2. The effect of presence of facultative bacteria species on semen and sperm quality of men seeking fertility care

    Directory of Open Access Journals (Sweden)

    C.A. Enwuru

    2016-09-01

    Conclusions: Although bacterial presence in semen reduced mean sperm concentration and viability, thereby contributed to oligozoospermia and by extension the chances of siring a child, however, factors other than bacterial presence may contribute more. Improved interpretative approaches of semen analyses are highlighted.

  3. Sensitive simultaneous detection of seven sexually transmitted agents in semen by multiplex-PCR and of HPV by single PCR.

    Directory of Open Access Journals (Sweden)

    Fabrícia Gimenes

    Full Text Available Sexually transmitted diseases (STDs may impair sperm parameters and functions thereby promoting male infertility. To date limited molecular studies were conducted to evaluate the frequency and type of such infections in semen Thus, we aimed at conceiving and validating a multiplex PCR (M-PCR assay for the simultaneous detection of the following STD pathogens in semen: Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Trichomonas vaginalis, Herpes virus simplex (HSV -1 and -2, and Treponema pallidum; We also investigated the potential usefulness of this M-PCR assay in screening programs for semen pathogens. In addition, we aimed: to detect human Papillomavirus (HPV and genotypes by single PCR (sPCR in the same semen samples; to determine the prevalence of the seven STDs, HPV and co-infections; to assess the possibility that these infections affect semen parameters and thus fertility. The overall validation parameters of M-PCR were extremely high including agreement (99.2%, sensitivity (100.00%, specificity (99.70%, positive (96.40% and negative predictive values (100.00% and accuracy (99.80%. The prevalence of STDs was very high (55.3%. Furthermore, associations were observed between STDs and changes in semen parameters, highlighting the importance of STD detection in semen. Thus, this M-PCR assay has great potential for application in semen screening programs for pathogens in infertility and STD clinics and in sperm banks.

  4. Intravaginal artificial insemination in bitches using frozen/thawed semen after dilution in powdered coconut water (ACP-106c).

    Science.gov (United States)

    Uchoa, D C; Silva, T F P; Mota Filho, A C; Silva, L D M

    2012-12-01

    The aim of this study was to evaluate powdered coconut water extender (ACP-106c; ACP Serviços Tecnológicos Ltda, ACP Biotecnologia, Fortaleza, Ceará, Brazil) as a diluent for freezing dog semen and the fertility after vaginal insemination of semen frozen therein. Ten ejaculates were collected from five dogs, evaluated fresh, diluted in ACP-106c, 10% egg yolk and 6% glycerol, cooled and frozen. In the first phase of the study, straws with frozen semen were thawed and immediately subjected to the same analysis as the fresh semen and, in addition, to Computer-Assisted Semen Analysis (CASA). In phase 2, 10 bitches that had been subjected to natural breeding during a preceding oestrous cycle were vaginally inseminated with thawed semen that had been re-diluted in ACP-106c. After thawing, a mean of 77% sperm motility was obtained through subjective analysis and 77.3% through CASA. Following artificial insemination, a 60% pregnancy rate was observed, resulting in a 50% parturition rate and a mean litter size of 3.4 (SEM 0.6), with 47.1% males and 52.9% females. ACP-106c can be successfully used for freezing canine semen, and vaginal deposition of such semen yields similar pregnancy rates to those reported in other studies. © 2012 Blackwell Verlag GmbH.

  5. Effect of semen collection method on sperm motility of gray wolves (Canis lupus) and domestic dogs (C. l. familiaris).

    Science.gov (United States)

    Christensen, Bruce W; Asa, Cheryl S; Wang, Chong; Vansandt, Lindsey; Bauman, Karen; Callahan, Margaret; Jens, Jackie K; Ellinwood, N Matthew

    2011-09-15

    Genetic management of Mexican gray wolves includes semen banking, but due to the small number of animals in the population and handling restrictions, improvements in semen collection and cryopreservation rely on results from studies of domestic dogs. Semen collection from wolves requires anesthesia and electroejaculation, which introduce potentially important variables into species comparisons, as dog semen is typically collected manually from conscious animals. To investigate possible effects of collection method on semen quality, we compared semen collection by the traditional manual method and by electroejaculation (EE) in a group of dogs (n = 5) to collection by EE only in wolves (n = 7). Samples were divided into two aliquots: neat or diluted in Tris/egg yolk extender, with motility evaluated at intervals up to 24 h. There were no differences (P > 0.10) in sperm motility in either neat or extended samples at 24 h from EE dogs and wolves, although motility of the wolf neat samples declined more rapidly (P < 0.05). However, there were differences (P < 0.01) between EE and manually collected dog semen in motility at 24 h, in both the neat and extended samples. Therefore, general motility patterns of dog and wolf semen collected by EE were similar, especially when diluted with a Tris/egg yolk extender, but sperm collected from dogs by EE did not maintain motility as long as manually collected samples, perhaps related to the longer exposure of EE samples to more prostate fluid. Copyright © 2011 Elsevier Inc. All rights reserved.

  6. Efektivitas Low Density Lipoprotein dan Kuning Telur Ayam dan Puyuh pada Pengawetan Semen Ayam Merawang (EFFECTIVESS OF LOW DENSITY LIPOPROTEIN AND EGG YOLK FROM CHICKEN AND QUAIL ON MERAWANG SEMEN PRESERVATION

    Directory of Open Access Journals (Sweden)

    Magfira Magfira

    2017-09-01

    Full Text Available The successful of artificial insemination (AI depends on the semen quality and extender. To minimize effect of cold shock during storage, extender is added with egg yolk. The objectives of this study were to compare the effectiveness of pure Low Density Lipoprotein (LDL and egg yolk from domestic chicken and quail on motility and longevity of Merawang chicken sperm. The semen was collected by massage method from three Merawang roosters. Immediately after collection, semen was evaluated macroscopically and microscopically. Only semen demonstrated >70% motility and 0.05 in spermatozoa longivity in the four diluents, with a range of longivities between 4.43 to 5.93 days. ABSTRAK Keberhasilan inseminasi buatan (IB salah satunya bergantung pada kualitas semen dan pengencer yang digunakan. Dalam meminimalisir pengaruh cold shock saat penyimpanan, pengencer ditambahkan dengan kuning telur. Tujuan dari penelitian ini adalah untuk membandingkan efektivitas Low Density Lipoprotein (LDL dan kuning telur yang berasal dari ayam kampung dan puyuh terhadap motilitas dan longivitas spermatozoa ayam. Koleksi semen dilakukan menggunakan metode pemijatan pada tiga ekor ayam merawang. Setelah semen dikoleksi, selanjutnya semen dievaluasi secara makroskopis dan mikroskopis. Semen yang menunjukkan motilitas 70% dan abnormalitas kurang dari 20% dibagi empat dan diencerkan menggunakan Ringer Laktat-LDLA (RL-LDLA, Ringer Laktat-(RL-LDLP, Ringer Laktatkuning telur ayam (RL-KTA, dan RL-kuning telur puyuh (RL-KTP. Semen yang telah diencerkan kemudian disimpan pada suhu 5oC. Motilitas spermatozoa diamati dua kali sehari sampai motilitas mencapai 0%. Motilitas spermatozoa dalam pengencer RL-LDLA berbeda dengan motilitas spermatozoa dalam pengencer RL-KTP pada jam ke-60 dan ke-72 (P0.05 dengan rentang longivitas antara 4,43 sampai 5,93 hari.

  7. Artificial insemination of cows with semen in vitro contaminated with Neospora caninum tachyzoites failed to induce neosporosis.

    Science.gov (United States)

    Canada, Nuno; Meireles, Carla Sofia; Ferreira, Paulo; Correia da Costa, José Manuel; Rocha, António

    2006-06-30

    Neosporosis is a major cause of abortion in cattle all over the world. Congenital transmission as well as horizontal transmission by ingestion of oocysts has been described. The detection of Neospora caninum DNA in bull semen warrants the investigation of possible transmission through the use of contaminated semen. In this experiment four cows were artificially inseminated with frozen-thawed semen contaminated in vitro with viable N. caninum tachyzoites (group A) and four control cows were inseminated with tachyzoites-free frozen-thawed semen, from the same bull (group B). Serum samples were collected 15 days before the artificial insemination (AI) and at days 10, 14, 21, 28, 45, 60 and 75 post-insemination. All sera samples were tested for neosporosis by direct agglutination test (DAT). Three of the cows from group A had negative DAT titers (neosporosis by artificial insemination with frozen-thawed semen is an unlikely event.

  8. [Comparative assessment of laboratory methods used in the analysis of semen].

    Science.gov (United States)

    Bukia, T; Maisuradze, K; Shanidze, L

    2006-12-01

    The purpose of the given study was to: - compare quantitative and qualitative indicators of the semen, examined with the use of the special sperm analyzer "SQA IIC-P", as well as through the methods of light microscopy; - and to select the parameters from both types of study, combined application of which would give the most optimal picture of the spermatogenesis of an examined individual. Twenty fertile and 65 childless men in the age range of 20-35 years were examined. Conclusion is given that the sperm analyzer "SQA IIC-P" accurately determines essential parameters of the reproduction function of men, however for targeted treatment it is critical to know a number of parameters, best revealed through microscopic examination of untreated and colored samples of semen. Therefore for obtaining a complete picture it is essential to combine examination of the semen both with the analyzer as well as through the use of microscopic methods.

  9. Potential reproduction and response of selenium and zinc mineral supplementation on quality of goat samosir semen

    Science.gov (United States)

    Siswoyo, P.; Tafsin, M.; Handarini, R.

    2018-02-01

    The present study was conducted to investigate the effect of suppllementattion of selenium and zinc on semen quality and growth of samosir goat. The experimental design used latin square design (4x4). The treatment supplementation mineral on multi nutrient block (MNB) composed of without sipplementation (p0), +10ppm selenium (p1), +10ppm zinc (p2), +10ppm selenium and +10ppm zinc (p3). The result showed that supplementation mineral selenium and zinc increased significantly (pselenium and zinc influenced motility, viability, volume concentration, and responding hypo osmotic swelling (HOS). Combination supplementation selenium and zinc significanly had higher semen quality than ither treatment. It is concluded that supplementation selenium and zinc improve growth and semen quality of samosir goat.

  10. Studies on inactivation of pathogenic microorganisms in culture media and in bovine semen by photosensitive agents.

    Science.gov (United States)

    Eaglesome, M D; Bielanski, A; Hare, W C; Ruhnke, H L

    1994-01-01

    The application of three photosensitive agents for disinfection of bovine semen was investigated. Bovine microbial pathogens suspended in tissue culture medium and/or PBS and also added to bovine semen were exposed to the photosensitive agents followed by irradiation. Hematoporphyrin, hematoporphyrin derivative and thiopyronine were effective against bovine herpes virus-1, bovine viral diarrhoea virus, Mycoplasma bovigenitalium, Mycoplasma canadense, and Ureaplasma diversum in culture media. In addition, thiopyronine was effective against Leptospira pomona. Similar treatments were not effective against Leptospira hardjo, Mycoplasma bovis, or Campylobacter fetus subsp. venerealis. When microorganisms were added to bovine semen, only bovine herpes virus-1 was controlled by the photosensitive agents used at concentrations which did not appear harmful to sperm cells.

  11. Spermatozoal transcripts expression levels are predictive of semen quality and conception rate in bulls (Bos taurus).

    Science.gov (United States)

    Parthipan, Sivashanmugam; Selvaraju, Sellappan; Somashekar, Lakshminarayana; Arangasamy, Arunachalam; Sivaram, Muniandy; Ravindra, Janivara Parameswaraiah

    2017-08-01

    Spermatozoal transcripts expression levels could be used to assess fertility potential of a male. The objective of the present study was to elucidate the predictive ability of the expression levels of growth, apoptosis and homeostasis regulating transcripts on sperm functions and fertility. The expression levels of spermatozoal RNA isolated from the neat semen samples were related to the good (discarded ejaculate, 40%, n = 6) quality semen producer and bulls (n = 12) with known conception rate. The relative fold expression levels of BMP2 were significantly (p conception rate (r = -0.57, p conception rate of the bull. The study provides ample evidence that the sperm transcripts expression levels might be used to predict quality semen production and bull fertility. Copyright © 2017 Elsevier Inc. All rights reserved.

  12. Semen evaluation in opium addicts and non addicts: A case-control study

    Directory of Open Access Journals (Sweden)

    eraj Shahramian

    2007-01-01

    Full Text Available Background: Opioids such as opium deteriorate sexual organs activities. The effect of opium on the spermatogenesis of men was assessed in a region where it is abused more than any opioids. Materials and methods: In this case–control study, we studied 224 subjects, divided into 2 groups: 112 opium dependant and 112 non-addicts. The aim of this study was to determine the effects of opium dependency on semen analysis. Results: There were significant differences between two groups in count, motility, morphology, viscosity and liquefaction. Conclusion: These results showed pathological effects of opium on semen analysis. This semen pathology is probably secondary to decreased testosterone production, with relative malfunction of the seminal tracts and the accessory glands.

  13. Race Differentiation Based on Raman Spectroscopy of Semen Traces for Forensic Purposes.

    Science.gov (United States)

    Muro, Claire K; Lednev, Igor K

    2017-04-18

    Several novel methods to determine externally visible characteristics of body fluid donors have been developed in recent years. These tests can help forensic investigators make predictions about the appearance of a suspect or victim, such as their sex, race, hair color, or age. While their potential benefit is undeniable, these methods destroy the physical evidence in the process. Raman spectroscopy has recently been used as a nondestructive technique to test for many of these characteristics. Here, we present the results from a study to determine the race of semen donors. Using Raman spectroscopy and multivariate data analysis, we were able to build a statistical model that accurately identified the race of all 18 semen donors in the calibration data set, as well as seven additional external validation donors. These results demonstrate Raman spectroscopy's potential to differentiate Caucasian and Black semen donors using chemometrics.

  14. Comparison of semen quality between university-based and private assisted reproductive technology laboratories

    DEFF Research Database (Denmark)

    Jensen, Christian Fuglesang S; Khan, Omar; Sønksen, Jens

    2018-01-01

    .9%). No significant differences were found in volume, concentration and total motile sperm count although the Bland-Altman plot bias for concentration was clinically significant (15.9 × 106/ml). CONCLUSIONS: In this small series, motility was significantly higher at private laboratories compared to a university......OBJECTIVE: Obtaining a semen analysis (SA) is an essential step in evaluating infertile men. Despite using standardized procedures for analysis semen quality in the same individual often varies on repeated tests. The objective of this study was to investigate inter-laboratory variation in semen...... quality between private- and university-based assisted reproductive technology (ART) laboratories. MATERIALS AND METHODS: IRB approval was obtained to retrospectively evaluate men with a SA at both the private- and university-based ART laboratories. When more than one SA was available from either...

  15. Manual semen collection from a Grevy's zebra stallion (Equus grevyi), onset of sperm production, semen characteristics, and cryopreservation of semen, with a comparison to the sperm production from a Grant's Zebra stallion (Equus burchelli boehmi).

    Science.gov (United States)

    Crump, J P; Crump, J W

    1994-01-01

    A manual technique was used to collect representative ejaculates from an unrestrained Grevy's zebra stallion beginning at 13 mo of age to determine the onset of sperm production, to calculate the number of spermatozoa produced per ejaculate, and to determine any seasonality associated with sperm production. Spermatozoa first appeared in the ejaculate at 31 mo of age. By 48 mo of age the zebra was producing up to 40 billion spermatozoa per ejaculate. Progressive sperm motility ranged from 75 to 95%. Gel-free semen volume averaged 75 to 120 ml/ejaculate. Gel volume ranged from 0 to 1100 ml/ejaculate. Semen was frozen in 2 different extenders in 0.5-ml PVC straws. The post-thaw motility of cryopreserved spermatozoa ranged from 30 to 70%. A domestic horse mare became pregnant on the first cycle after insemination with frozen-thawed spermatozoa from this zebra. Sperm production data obtained from semen collections made on a Grant's Zebra stallion from 3 to 8 yr of age is presented for comparison of the 2 species.

  16. Subfertility Increases Risk of Testicular Cancer: Evidence from Population-Based Semen Samples

    Science.gov (United States)

    Hanson, Heidi A; Anderson, Ross E; Aston, Kenneth I; Carrell, Douglas T; Smith, Ken R; Hotaling, James M

    2015-01-01

    Objective To further understand the association between semen quality and cancer risk using well-defined semen parameters. Design Retrospective cohort study. Setting Subfertility Heath and Assisted Reproduction (SHARE) study in Utah from 1994 to 2011. Patients 20,433 men from that underwent semen analysis (SA) and a sample of 20,433 fertile controls matched on age and birth year Interventions none. Main Outcome Measures Risk of all cancers, as well as site-specific results for prostate, testicular, and melanoma. Results Relative to fertile men, men with SA have an increased risk of testicular cancer (Hazard Rate Ratio (HR) =3.3). When the characterization of infertility is refined using individual semen parameters, we find that oligozoospermic men have an increased risk of cancer relative to fertile controls. This association is particularly strong for testicular cancer, with increased risk in men with oligozoospermia based on concentration (HR=11.9) and sperm count (HR=10.3). Men in the in the lowest quartile of motility (HR=4.1), viability (HR=6.6), morphology (HR=4.2) or total motile count (HR=6.9) have higher risk of testicular compared to fertile men. Men with sperm concentration and count in the 90th percentile of the distribution (≥178 M/ml and ≥579, respectively) and total motile count (TMC) have an increased risk of melanoma (HRConcentration=2.1; HRCount=2.7; HRTMC=2.0). We find no differences in cancer risk between azoospermic and fertile men. Conclusions Men with SA have an increased risk of testicular cancer that varies by semen quality. Unlike prior work, we did not find an association between azoospermia and increased cancer or testicular cancer risk. Capsule Subfertile men have an increased risk of testicular cancer that varies by semen quality. We did not find an association between azoospermia and increased cancer or testicular cancer risk. PMID:26604070

  17. Decline of semen quality and increase of leukocytes with cigarette smoking in infertile men

    Directory of Open Access Journals (Sweden)

    Zhi Hong Zhang

    2013-01-01

    Full Text Available Background: Previous researches about the effect of smoking on semen quality are contradictory, and the mechanism behind the harmful effect of smoking on semen quality still remains unclear until today. Objective: The objectives of this study are evaluation of the relationship between smoking and fertility, investigation of the effects of cigarette smoking on sperm parameters and detection of presence of leukocytes within the semen of idiopathic infertile men from Northeastern China. Materials and Methods: A retrospective study of 1512 infertile patients who visited affiliated hospitals of Jilin University from 2007-2010 were enrolled in this study. Patients were assigned into one non-smoking and one smoking group which was divided into mild, moderate and heavy subgroups. Sperm parameters (including leukocytes and sperm morphology analysis were performed using standard techniques. Results: Compared with non-smokers, smokers had a significant decrease in semen volumes (p=0.006, rapid progressive motility (p=0.002 and sperm viability (p=0.019; moreover, smokers had a significant increase in the levels of immotile sperms (p=0.005 and semen leukocytes (p=0.002; pH and sperm concentration were not statistically significant (p=0.789 and p=0.297 respectively. Sperm motion parameters were all lower in the smokers except for beat-cross frequency (Hz (BCF. Further, the percentage of normal morphology sperm was decreased significantly in smokers (p=0.003, the sperm morphology was worse with increasing degree of smoking. Conclusion: These findings suggest that smoking leads to a significant decline in semen quality and higher levels of leukocytes, thus smoking may affects the fertilization efficiency.

  18. SEMEN PRODUCTION AND PRODUCTIVE LIFE OF SAHIWAL BULLS: RELATIONSHIP WITH GENETIC WORTH

    Directory of Open Access Journals (Sweden)

    M. S. KHAN, A. A. BHATTI, S. A. BHATTI1 AND M. ASHIQ2

    2007-01-01

    Full Text Available Objective of the present study was to document the semen producing ability, productive life and genetic ability for lactation milk yield of Sahiwal bulls used for artificial insemination (AI in Punjab and to find the impact of AI bulls on the improvement of Sahiwal cattle. Data from Semen Production Unit (SPU, Qadirabad, Sahiwal, Pakistan were used for this purpose. A repeatability animal model was used for estimation of breeding values for lactation milk yield. Productive life of a bull was calculated as a difference between culling age and the age at first ejaculation. Number of bulls brought to SPU varied from 9 to 102 for any year. Average number of doses of semen produced by any bull for a year varied from 724 to 5745. On the average, 238 bulls produced 17143 ± 1164 semen doses during their average stay of 5.4 ± 0.2 years. About 50% of the bulls stayed for less than four years at the SPU; with a maximum range of 14 years. Progeny tested bulls (n=90 produced 5000 and 10000 semen doses (Y in three and four years of stay (X, respectively (Y = 24.8 + 2.3635 X - 0.0112 X2. To produce 20,000 doses, it is predicted that bulls need to stay for six and a half years at the SPU. There was no association between breeding values for lactation milk yield estimated under a repeatability animal model (EBVs and number of semen doses produced (r = 0.17 and EBVs and number of daughters. Lack of genetic superiority of bulls used indicated that AI did not bring desired genetic improvement in Sahiwal cattle in the present situation. Modifications for judicious utilization of bulls are suggested along with improvements in data recording.

  19. Semen evaluation and fertility assessment in a purebred dog breeding facility.

    Science.gov (United States)

    Hesser, Andrea; Darr, Christa; Gonzales, Kris; Power, Heather; Scanlan, Tawny; Thompson, James; Love, Charles; Christensen, Bruce; Meyers, Stuart

    2017-01-01

    Semen quality in dogs has not been assessed in a longitudinal study that includes endpoints of female fertility and pregnancy. Although use of artificial insemination with chilled semen is increasingly used in canine reproduction, the resultant level of predictability and odds of fertile matings for dogs is still not fully understood. This research provides, for the first time, comprehensive semen evaluation in a large population of dogs in which fertility has been tracked. Duplicate ejaculates were obtained from 39 Labrador retriever males of the Guide Dogs for the Blind (San Rafael, CA, USA) breeding program. Sperm endpoints were determined in fresh semen and extended chilled semen at 48 hour after collection. Evaluation included total and progressive motility, average path velocity, morphology, membrane lipid peroxidation, presence of sperm reactive oxygen species, sperm chromatin structure, and mitochondrial DNA copy number. Male age ranged from 1 to 10 years and were grouped as young (Y; 1-3 years, n = 21), middle aged (M; 4-6 years, n = 13), and senior (S; 7 years or greater, n = 5) for analysis. The effects of age and sperm state (fresh vs. chilled) on the above sperm endpoints were determined using a linear mixed effects model. Semen endpoint values for all parameters were established for this group of fertile males. Progressive motility was only lower in the senior male chilled samples compared to all other groups, fresh and chilled (P dogs compared with the other age groups (P reproduction. Copyright © 2016 Elsevier Inc. All rights reserved.

  20. Comparison of the effect of two freeze-thawing curves for porcine semen. Preliminary results

    Directory of Open Access Journals (Sweden)

    Caldevilla M

    2016-12-01

    Full Text Available Results obtained in fertility and litter size using frozen-thawed porcine semen are far from those obtained with natural service or artificial insemination of cooled semen. The objective of this study was to evaluate freeze-thawing of porcine semen comparing the traditional slow method to a rapid curve of temperature descent, using two cryoprotectants. Six males of proven fertility (n=6, r=2 were used. Semen was obtained using the gloved-hand technique and was transported to the laboratory at 17 ºC diluted in Androstar® plus. Samples were centrifuged 15 minutes at 800 g and re-diluted in: a 5% dimethylformamide, 11% lactose, 20% egg yolk, 0.5% Equex or b 3% glycerol, 11% lactose, 20% egg yolk, 0.5% Equex. The semen was frozen in 0.5 ml straws up to a final concentration of 300 millions sperm /ml using either a modified slow traditional Westendorff curve or a rapid curve. In both cases thawing was carried out at 37 ºC during 1 minute. Kinetic motility parameters were evaluated using a CASA system (ISAS v1, Proiser®, Spain. Sperm viability and acrosome status were evaluated using the FITC-PNA/PI stain. The results were analyzed using a factorial design (analysis ofvariance with two factors, with two levels for each one and using the male as a blocking factor. No interaction was observed between the parameters. No significant differences (p> 0.05 were observed between curves or between cryoprotectants neither in any of the kinetic motility parameters evaluated nor in sperm viability and acrosome status. No significant differences (p> 0.05 were observed between curves or between cryoprotectants in sperm morphology in thawed porcine semen. Taking into account the results obtained, the rapid curve would be the practical choice as it is, faster and more manageable for fieldwork in any pig farm.

  1. The Semen pH Affects Sperm Motility and Capacitation.

    Science.gov (United States)

    Zhou, Ji; Chen, Li; Li, Jie; Li, Hongjun; Hong, Zhiwei; Xie, Min; Chen, Shengrong; Yao, Bing

    2015-01-01

    As the chemical environment of semen can have a profound effect on sperm quality, we examined the effect of pH on the motility, viability and capacitation of human sperm. The sperm in this study was collected from healthy males to avoid interference from other factors. The spermatozoa cultured in sperm nutrition solution at pH 5.2, 6.2, 7.2 and 8.2 were analyzed for sperm total motility, progressive motility (PR), hypo-osmotic swelling (HOS) rate, and sperm penetration. Our results showed that these parameters were similar in pH 7.2 and 8.2 sperm nutrition solutions, but decreased in pH 5.2 and 6.2 solutions. The HOS rate exhibited positive correlation with the sperm total motility and PR. In addition, the sperm Na(+)/K(+)-ATPase activity at different pHs was measured, and the enzyme activity was significantly lower in pH 5.2 and 6.2 media, comparing with that in pH 8.2 and pH 7.2 solutions. Using flow cytometry (FCM) and laser confocal scanning microscopy (LCSM) analysis, the intracellular Ca2(+ )concentrations of sperm cultured in sperm capacitation solution at pH 5.2, 6.2, 7.2 and 8.2 were determined. Compared with that at pH 7.2, the mean fluorescence intensity of sperm in pH 5.2 and 6.2 media decreased significantly, while that of pH 8.2 group showed no difference. Our results suggested that the declined Na(+)/K(+)-ATPase activity at acidic pHs result in decreased sperm movement and capacitation, which could be one of the mechanisms of male infertility.

  2. High-expression EGFR/cell membrane chromatography-online-high-performance liquid chromatography/mass spectrometry: rapid screening of EGFR antagonists from Semen Strychni.

    Science.gov (United States)

    Sun, Meng; Guo, Ying; Dai, Bingling; Wang, Chen; He, Langchong

    2012-09-15

    Traditional methods for screening active compounds from complex system such as traditional Chinese medicines are relatively cumbersome and time-consuming. In order to improve this situation, we established an online analytical method for screening, separation and identification EGFR antagonists from traditional Chinese medicines, which is described in this study. Cells with high EGFR expression levels were used to prepare the cell membrane stationary phase for the EGFR cell membrane chromatography model with the purpose of screening active compounds. Separation of the retention fractions was achieved by the high-performance liquid chromatography, and identification was conducted via electrospray ionization quadrupole mass spectrometry. The inhibitory effects of active compounds on EGFR cell growth were also demonstrated in vitro. The screening results showed that vauquline and strychnine from Semen Strychni could be active components acting on EGFR similarly to gefitinib as a control drug. Results from biological trials showed that vauquline and strychnine inhibited cell proliferation of HEK293/EGFR cells, and inhibited Erk phosphorylation, and can effectively reduce expression of downstream signaling molecules. This EGFR cell membrane chromatography-online-high-performance liquid chromatography/tandem mass spectrometry method can be applied for rapid screening, separation and identification of EGFR antagonists from traditional Chinese medicines and should be useful for drug discovery with natural medicinal herbs. Copyright © 2012 John Wiley & Sons, Ltd.

  3. The effect of cigarette smoking on semen quality of infertile men

    International Nuclear Information System (INIS)

    Mehrannia, T.

    2007-01-01

    To evaluate the effects of cigarette smoking on semen quality of infertile men. Two hundred fourteen infertile men who had been smoking cigarette and one hundred thirty infertile non smokers men participated in this study. Seminal volume, sperm concentration, motility, viability, and morphology were examined. The quality of spermatozoa obtained from smokers were much lower than non-smokers (P<0.01). The sperm concentration, viability and forward progression were negatively correlated with cigarette smoking (P<0.01). Smoking does affect the semen quality of infertile men. (author)

  4. Effect of dialysis of dog semen on sperm characteristics and some biochemical components of seminal plasma.

    Science.gov (United States)

    Strzeżek, R; Koziorowska-Gilun, M; Kiełczewski, K; Kordan, W

    2015-01-01

    The aim of this study was to investigate the effect of dog semen dialysis on sperm characteristics and some biochemical components of seminal plasma. Whole ejaculates were dialyzed against Tris-citrate-fructose extender for a 5 h period at room temperature (using semi-permeable cellulose tubing of 12-14 kDa molecular weight cut-off). It has been demonstrated that the long-term dialysis of dog semen causes a significant decrease in sperm quality parameters and disrupts the biochemical properties of seminal plasma. This procedure requires further improvement.

  5. Survival of chlamydiae in human semen prepared for artificial insemination by donor

    DEFF Research Database (Denmark)

    Thorsen, Poul; Møller, Birger R.; Halkier-Sørensen, Lars

    1991-01-01

    Semen specimens from 21 men with urethral infection with Chlamydia trachomatis were tested for the presence of the organism before and after cryopreservation for 3 weeks of storage at -196 degrees C. Five specimens were chlamydia-positive before preservation and four of them were still positive...... after storage when examined by enzyme immunoassay (Chlamydiazyme). When examined by cell culture, four proved chlamydia- positive before storage and two afterwards. The results indicate that testing for C. trachomatis has to be performed from the urethra of all donors of semen used for artificial...

  6. Effects of He-Ne laser irradiation on the storage of turkey semen

    Directory of Open Access Journals (Sweden)

    S. Passarella

    2011-03-01

    Full Text Available Maintenance or improvement of sperm quality during storage could prevent the loss of fertilizing capacity associated with stored turkey semen. Therefore the optimization of stored turkey semen could be useful to breeder industry since the commercial production of this bird relies almost entirely on artificial insemination. Previous research have shown that He-Ne laser irradiation in mammalian sperm increased the motility (Stato, 1986, decreased the mortality, promoted the acrosome reaction, which have a pivotal role in assisted fecundating programmes as therapy for resolving infertility in domestic animals..........

  7. Quality of fresh and chilled-stored raccoon dog semen and its impact on artificial insemination efficiency.

    Science.gov (United States)

    Jarosz, Łukasz; Grądzki, Zbigniew; Kalinowski, Marcin; Laskowska, Ewa

    2016-10-10

    The aim of the study was to evaluate the quality of fresh raccoon dog semen and raccoon dog semen stored at 4 °C. The qualitative evaluation was based on apoptosis in the sperm cells, which was tested by the Annexin V/Pi assay, the TUNEL method and JC-1. In addition, the suitability of the semen for insemination and its effect on reproduction in females were determined in relation to the time of storage. During cold storage of the semen, in the samples from all groups a gradual decrease was noted in the percentage of live cells, and an increase in the percentage of cells with abnormal morphology, exhibiting changes typical of late-stage apoptosis (V + /PI + ), and of necrotic cells (V - /PI + ). There was a significant increase in the percentage of ApoBrDu + sperm cells, while the mitochondrial membrane potential of the sperm decreased significantly after 12 h of storage at 4 °C in the case of lower-quality semen and after 48 h in the case of semen of good quality. As the percentage of sperm with DNA and cell membrane damage increased and the mitochondrial membrane potential decreased, there was an increase in AspAT and acrosin activity. The increase in the percentage of apoptotic sperm in the raccoon dog semen stored at 4 °C resulted in a decrease in the number of females with cubs. Identification of apoptotic changes in sperm by flow cytometry using the annexin assay, the TUNEL assay and evaluation of mitochondrial membrane potential can be recommended for determination of the suitability of raccoon dog semen for artificial insemination. The study shows that fresh raccoon dog semen should not be used for insemination more than 48 h after collection in the case of semen of very high quality, or after more than 24 h in the case of semen of poorer quality. Cytometric methods of semen analysis should also be used to evaluate various extenders of raccoon dog semen and methods of cryopreservation in terms of ensuring sperm viability, fertilization

  8. Recommended foods for male infertility in Iranian traditional medicine.

    Science.gov (United States)

    Nejatbakhsh, Fatemeh; Nazem, Esmaeil; Goushegir, Ashrafeddin; Isfahani, Mohammad Mahdi; Nikbakht Nasrabadi, Alireza; Baygom Siahpoosh, Marzieh

    2012-11-01

    Male infertility accounts for 30-50% of all infertilities among couples. Iranian traditional medicine (ITM) stressed the importance of nutrition in the prevention and treatment of male infertility. Many Iranian traditional physicians have described the traits of specific foods for prevention and treatment of male infertility. To explore the principles and roles of foods recommended by ITM scientists in prevention and treatment of male infertility as well as enlisting all the recommended foods for treating this problem addressed through the ITM original resources written between 815 and 1901. In this review study specific data related to the subject among all referral ITM texts was extracted firstly, and then the collected data were analyzed using inductive content analysis. The analysis of data revealed that foods that enhance sexual performance must have 3 properties; they should be warm in nature, very nutritious, and flatulent. Foods that are warm in nature and nutritious affect the quality and quantity of semen. A food having the third trait of being flatulent is required to complete sexual performance by creating an erection. Foods with only one of these traits must be consumed with another food that has the other trait. This study also provided a list of foods that can enhance the quality and increase the quantity of semen. Foods that can enhance sexual performance and the quality and quantity of semen can be recommended to male patients who suffer from infertility in medical centers.

  9. Diabetes Medicines

    Science.gov (United States)

    Diabetes means your blood glucose, or blood sugar, levels are too high. If you can't control your diabetes with wise food choices and physical activity, you may need diabetes medicines. The kind of medicine you take depends ...

  10. Herbal Medicine

    Science.gov (United States)

    ... for its scent, flavor, or therapeutic properties. Herbal medicines are one type of dietary supplement. They are ... and fresh or dried plants. People use herbal medicines to try to maintain or improve their health. ...

  11. Sperm motility patterns in Andalusian donkey (Equus asinus) semen: effects of body weight, age, and semen quality.

    Science.gov (United States)

    Dorado, J; Acha, D; Gálvez, M J; Ortiz, I; Carrasco, J J; Díaz, B; Gómez-Arrones, V; Calero-Carretero, R; Hidalgo, M

    2013-04-15

    substantial increase in information acquired during donkey semen analysis. Copyright © 2013 Elsevier Inc. All rights reserved.

  12. Polyphenolics from Albizia harveyi Exhibit Antioxidant Activities and Counteract Oxidative Damage and Ultra-Structural Changes of Cryopreserved Bull Semen

    Directory of Open Access Journals (Sweden)

    Mansour Sobeh

    2017-11-01

    Full Text Available Albizia harveyi is a tropical deciduous tree, found across South and Eastern Africa and widely used in traditional medicine. The leaf extract ameliorated the damaging effects of the frozen-thawing process in cryopreserved bull semen. In a dose-dependent pattern, sperm motility, viability, and membrane integrity were improved compared to the untreated control. Furthermore, the extract increased the percentage of viable sperm cells and reduced the percentages of early apoptotic and apoptotic sperm cells as well as the damage in sperm ultra-structure. These activities are in agreement with the robust antioxidant properties in vitro and in the seminal fluid as observed in the total antioxidant capacity and the lipid peroxidation parameter malondialdehyde. LC-MS yielded 35 compounds. The extract was dominated by quercetin-O-galloyl-hexoside and quercetin-O-pentoside, along with other flavonoid glycosides. The polyphenols are probably responsible for the observed activities. In conclusion, the current findings show that A. harveyi leaves are rich in bioactive polyphenols with functional properties, validating its traditional use.

  13. Cryopreservation of boar semen. II: Effect of cooling rate and duration of freezing point plateau on boar semen frozen in mini- and maxi-straws and plastic bags.

    Science.gov (United States)

    Bwanga, C O; Einarsson, S; Rodriguez-Martinez, H

    1991-01-01

    The post-thaw motility and the acrosome integrity of semen from 4 boars frozen with a programmable freezing machine, in mini (0.25 ml) and maxi (5 ml) plastic straws and in 10 x 5 cm Teflon FEP-plastic bags (0.12 mm thick, 5 ml), were compared. The freezing of the semen was monitored by way of thermo-couples placed in the straws and the bags. Three freezing programmes were used, namely A: from +5 degrees C, at a rate of 3 degrees C/min, to -6 degrees C, held for 1 min at -6 degrees C, and followed by a cooling rate of 20 degrees C/min to -100 degrees C; B: a similar curve except that there was no holding time at -6 degrees C and that the cooling rate was 30 degrees C/min, and C: from +5 degrees C to -100 degrees C, with a cooling rate of 35 degrees C/min, followed by storage in liquid N2. Despite the freezing curve assayed, both the mini-straws and the bags depicted much shorter freezing point plateaus as compared to the maxi-straws. Post-thaw sperm motility as well as the amount of normal apical ridges were equally significantly higher when semen was frozen in mini-straws or in bags than in maxi-straws. Significant differences in these post-thawing parameters were obtained between the freezing curves used. The stepwise freezing procedure A appeared as the best alternative for boar semen, considering this in vitro evaluation.

  14. Quality assessment of crude and processed Arecae semen based on colorimeter and HPLC combined with chemometrics methods.

    Science.gov (United States)

    Sun, Meng; Yan, Donghui; Yang, Xiaolu; Xue, Xingyang; Zhou, Sujuan; Liang, Shengwang; Wang, Shumei; Meng, Jiang

    2017-05-01

    Raw Arecae Semen, the seed of Areca catechu L., as well as Arecae Semen Tostum and Arecae semen carbonisata are traditionally processed by stir-baking for subsequent use in a variety of clinical applications. These three Arecae semen types, important Chinese herbal drugs, have been used in China and other Asian countries for thousands of years. In this study, the sensory technologies of a colorimeter and sensitive validated high-performance liquid chromatography with diode array detection were employed to discriminate raw Arecae semen and its processed drugs. The color parameters of the samples were determined by a colorimeter instrument CR-410. Moreover, the fingerprints of the four alkaloids of arecaidine, guvacine, arecoline and guvacoline were surveyed by high-performance liquid chromatography. Subsequently, Student's t test, the analysis of variance, fingerprint similarity analysis, hierarchical cluster analysis, principal component analysis, factor analysis and Pearson's correlation test were performed for final data analysis. The results obtained demonstrated a significant color change characteristic for components in raw Arecae semen and its processed drugs. Crude and processed Arecae semen could be determined based on colorimetry and high-performance liquid chromatography with a diode array detector coupled with chemometrics methods for a comprehensive quality evaluation. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  15. Male caffeine and alcohol intake in relation to semen parameters and in vitro fertilization outcomes among fertility patients.

    Science.gov (United States)

    Karmon, A E; Toth, T L; Chiu, Y-H; Gaskins, A J; Tanrikut, C; Wright, D L; Hauser, R; Chavarro, J E

    2017-03-01

    Much of the literature on the impact of male caffeine and alcohol intake on reproductive outcomes has utilized semen quality as a proxy for male fertility, although semen parameters have a limited predictive value for spontaneous pregnancy. The objective of this study was to investigate whether male caffeine and alcohol intakes are associated with semen parameters and assisted reproductive technology outcome. The Environment and Reproductive Health Study, an ongoing prospective cohort study, enrolls subfertile couples presenting for treatment at an academic fertility center (2007-2012). A total of 171 men with 338 semen analyses and 205 assisted reproductive technology cycles were included in this analysis. Diet was assessed using a 131-item food frequency questionnaire. Mixed models adjusting for potential confounders were used to evaluate the relationships of male caffeine and alcohol intakes with semen parameters and assisted reproductive technology outcomes. There was no association between male caffeine and alcohol intake and semen quality. Male caffeine intake was negatively related to live birth after assisted reproductive technologies (p-trend caffeine intake (≥272 mg/day) compared to couples with a male partner in the lowest quartile of intake (caffeine and alcohol intakes were associated with live birth after assisted reproductive technologies, but not with semen parameters, among fertility patients. © 2017 American Society of Andrology and European Academy of Andrology.

  16. Effects of Different Levels of Pigeon Egg Yolk in Extenders on the Post-Thaw Semen Quality of Sahiwal Bulls

    Directory of Open Access Journals (Sweden)

    Hafez Jamil-ur-Rahman, Nazir Ahmad*, Najib-ur-Rahman, Salman Waheed, Maqbool Ahmad, Muhammad Younis1 and Tanveer Ahmad2

    2012-06-01

    Full Text Available In this study, effects of replacing chicken egg yolk (CEY with pigeon egg yolk (PEY in extenders on post-thaw semen quality in Sahiwal bulls were investigated. Attempts were also made to see if post thaw semen quality was affected by reducing PEY level in the extender. Twenty four semen samples were diluted with five Tris-based extenders. Extender A contained 20% CEY and was used as control, while extenders B, C, D and E contained 5, 10, 15 and 20% PEY, respectively. After freezing and storage for 24 hrs in liquid nitrogen, these samples were evaluated for post-thaw semen quality parameters.The difference in post extension sperm motility between extenders A (20% CEY and E (20% PEY was non significant. Post extension sperm motility decreased as the level of PEY in the extender was decreased. A similar trend was recorded for post thaw sperm motility, livability, absolute index of livability and sperm with intact plasma membrane. The percentages of spermatozoa with abnormal head, or tail were lower (P<0.01 in control extender A and extender E compared to extenders B, C and D. However, for abnormal mid-piece, extenders A and E showed lower values than extender C only. It was concluded that replacing CEY with PEY in same concentration (20% did not improve post thaw semen quality. Moreover, reducing the concentration of PEY in semen extender from 20 to 5% had adverse effects on post-thaw quality of Sahiwal bull semen.

  17. Effect of oral administration of Tribulus terrestris extract on semen quality and body fat index of infertile men.

    Science.gov (United States)

    Salgado, R M; Marques-Silva, M H; Gonçalves, E; Mathias, A C; Aguiar, J G; Wolff, P

    2017-06-01

    Male fertility can be evaluated through complete semen analysis. Plants belonging to the Tribulus genus are known for their role in enhancing sex hormone levels and semen quality. The aim of this study was to evaluate the effects of T. terrestris on semen quality and physiological parameters. Sixty-five men with abnormal semen evaluation were included in this study, in which they were prescribed with oral administration of Androsten ® (250 mg of Tribulus terrestris dried extract per capsule). Body fat percentage, lean muscle mass gain, fluctuation in steroid hormone levels and all semen parameters were analysed during the period of treatment. The results demonstrated that decrease in the percentage of body fat and increase in lean mass were significant, as well as increase in dihydrotestosterone levels. Complete semen analysis evaluated at the end of treatment showed significant enhancement in sperm concentration, motility and liquefaction time. Protodioscin, the main phytochemical agent of the Tribulus genus, acts on sertoli cells, germ cell proliferation and growth of seminiferous tubules. This component is known to convert testosterone into dihydrotestosterone, which plays important roles in male attributes. Our results indicate the therapeutic use of Tribulus terrestris by men presenting altered semen parameters, and/or undergoing infertility treatment. © 2016 Blackwell Verlag GmbH.

  18. Immunological Tolerance, Pregnancy, and Preeclampsia: The Roles of Semen Microbes and the Father†

    Science.gov (United States)

    Kenny, Louise C.; Kell, Douglas B.

    2018-01-01

    Although it is widely considered, in many cases, to involve two separable stages (poor placentation followed by oxidative stress/inflammation), the precise originating causes of preeclampsia (PE) remain elusive. We have previously brought together some of the considerable evidence that a (dormant) microbial component is commonly a significant part of its etiology. However, apart from recognizing, consistent with this view, that the many inflammatory markers of PE are also increased in infection, we had little to say about immunity, whether innate or adaptive. In addition, we focused on the gut, oral and female urinary tract microbiomes as the main sources of the infection. We here marshall further evidence for an infectious component in PE, focusing on the immunological tolerance characteristic of pregnancy, and the well-established fact that increased exposure to the father’s semen assists this immunological tolerance. As well as these benefits, however, semen is not sterile, microbial tolerance mechanisms may exist, and we also review the evidence that semen may be responsible for inoculating the developing conceptus (and maybe the placenta) with microbes, not all of which are benign. It is suggested that when they are not, this may be a significant cause of PE. A variety of epidemiological and other evidence is entirely consistent with this, not least correlations between semen infection, infertility and PE. Our view also leads to a series of other, testable predictions. Overall, we argue for a significant paternal role in the development of PE through microbial infection of the mother via insemination. PMID:29354635

  19. Reduced Postvasectomy Semen Analysis Testing With the Implementation of Special Clearance Parameters.

    Science.gov (United States)

    Bieniek, Jared M; Fleming, Tyler B; Clark, Joseph Y

    2015-09-01

    To determine the applicability of postvasectomy special clearance parameters (<100,000 nonmotile sperm/mL on semen analysis) suggested by the American Urological Association and to define the associated cost savings with avoidance of further testing. We retrospectively reviewed the cohort of men undergoing vasectomy from December 2009 to August 2012 at a single institution. Patient demographics and postvasectomy semen analysis (PVSA) results were collected for clearance parameter comparisons. During the study period, 230 patients underwent vasectomy with a mean ± SD age of 36.4 ± 6.5 years. Among the cohort, 83.5% were married and 95.2% had one or more children. The initial PVSA was completed by 111 (48.3%) patients at a mean of 17.8 weeks (range 4-45) following vasectomy. Sperm was identified on initial PVSA in 40 patients (36.0%); 1 patient was found to have motile sperm. Of 39 patients, 38 (97.4%) with nonmotile sperm on PVSA could be cleared to cease other contraceptives based on the most recent clearance guidelines. For those completing an initial PVSA, postvasectomy clearance increased from 64.0% to 98.2% representing a potential cost savings of $2356 in repeat semen testing. Postvasectomy contraceptive clearance can be greatly increased when rare nonmotile sperm parameters are included although postvasectomy semen testing compliance remains poor. Copyright © 2015 Elsevier Inc. All rights reserved.

  20. Recent adverse trends in semen quality and testis cancer incidence among Finnish men

    DEFF Research Database (Denmark)

    Jorgensen, N.; Vierula, M.; Jacobsen, R.

    2011-01-01

    Impaired semen quality and testicular cancer may be linked through a testicular dysgenesis syndrome of foetal origin. The incidence of testis cancer has been shown to increase among Finnish men, whereas there is no recent publication describing temporal trends in semen quality. Therefore, we...... carried out a prospective semen quality study and a registry study of testis cancer incidence among Finnish men to explore recent trends. A total of 858 men were investigated in the semen quality study during 1998-2006. Median sperm concentrations were 67 (95% CI 57-80) million/mL, 60 (51-71) and 48 (39......-60) for birth cohorts 1979-81, 1982-83 and 1987; total sperm counts 227 (189-272) million, 202 (170-240) and 165 (132-207); total number of morphologically normal spermatozoa 18 (14-23) million, 15 (12-19) and 11 (8-15). Men aged 10-59 years at the time of diagnosis with testicular cancer during 1954-2008 were...

  1. Healthy overweight male partners of subfertile couples should not worry about their semen quality

    NARCIS (Netherlands)

    Duits, Floor H.; van Wely, Madelon; van der Veen, Fulco; Gianotten, Judith

    2010-01-01

    Objective: To study the effect of body mass index (BMI) on semen quality in a cohort of male partners in subfertile couples. Design: Prospective cohort study. Setting: A fertility center based in an academic hospital. Patient(s): Between January 2000 and January 2007, 1466 men visiting the Centre

  2. Effect of L-carnitine supplementation on drake semen quality | Al ...

    African Journals Online (AJOL)

    This study was conducted to determine the effect on semen quality traits of supplementing the diets of Iraqi drakes with L-carnitine. Forty eight male Iraqi ducks, 30 weeks old, were randomly allocated to four treatments with 12 drakes per treatment group, replicated three times, with four drakes per replicate. The treatment ...

  3. Results of artificial insemination at home by the partner with cryopreserved donor semen: a randomized study

    NARCIS (Netherlands)

    Hogerzeil, H. V.; Hamerlynck, J. V.; van Amstel, N.; Nagelkerke, N. J.; Lammes, F. B.

    1988-01-01

    The use of cryopreserved semen offers the possibility of home insemination by the instructed partner. A comparative study was designed whereby participants were randomly allocated to use home or clinic insemination for six cycles. If no pregnancy had occurred after six cycles, the site of

  4. The effects of gelatin supplementation prior to cooling on ram semen quality and fertility.

    Science.gov (United States)

    The physical and chemical characteristics of gelatin have been used to justify its inclusion in extenders to preserve the sperm quality and improve results of cervical artificial insemination with cooled semen. The objective of this study was to evaluate the effect of gelatin supplementation in cool...

  5. Physiologic and pathologic levels of reactive oxygen species in neat semen of infertile men.

    Science.gov (United States)

    Desai, Nisarg; Sharma, Rakesh; Makker, Kartikeya; Sabanegh, Edmund; Agarwal, Ashok

    2009-11-01

    To define physiologic levels of reactive oxygen species in infertile men and establish a cutoff value of reactive oxygen species level in neat semen with a high sensitivity and specificity to differentiate infertile men from fertile donors (controls). Reactive oxygen species levels were measured in the neat semen samples (n = 51) from fertile donors and infertile patients (n = 54). Reproductive research laboratory at a tertiary care hospital. Infertile patients from male infertility clinic. Reactive oxygen species measurement in neat semen sample using luminol-based chemiluminescence method, receiver operating characteristic curves. Seminal reactive oxygen species levels, cutoff value, sensitivity and specificity, positive and negative predictive values. The best cutoff value to distinguish between healthy fertile donors and infertile men was 0.0185 x 10(6) counted photons per minute/20 x 10(6) sperm. At this threshold, the specificity was 82% and the sensitivity was 78%. This value can be defined as basal reactive oxygen species level in infertile men. Reactive oxygen species levels in neat semen samples as measured by luminol-based chemiluminescence are a highly specific and sensitive test in the diagnosis of infertility. This test also may help clinicians treat patients with seminal oxidative stress.

  6. Cadmium, Chromium, and Copper Concentration plus Semen-Quality in Environmental Pollution Site, China.

    Science.gov (United States)

    Li, Yan; Gao, Qiaoyan; Li, Mingcai; Li, Mengyang; Gao, Xueming

    2014-01-01

    The environmental pollution is one of the factors contributing to the decrease of sperm quality for human beings. The aim of this study was to assess cadmium (Cd), chromium (Cr), and copper (Cu) concentration of man in environmental pollution site, and explore relationships between men exposure to Cd, Cr, and Cu and semen-quality parameters in environmental pollution site. Ninety five men were recruited through pollution area and controls in 2011. We measured semen quality using Computer-aided Semen Quality Analysis, and Cd, Cr, and Cu levels in seminal plasma using Graphite Gurnace Atomic Absorption Spectroscopy. Spearman rank correlation analysis was used to evaluate the correlation between Cd, Cr and Cu concentration in seminal plasma and semen quality. The mean of seminal plasma Cd, Cr, and Cu values in pollution area was higher than the controls. Seminal plasma Cr values displayed a significant negative correlation with total motility and normomorph sperm rate. Seminal plasma Cu values also displayed a negative correlation with normomorph sperm rate. Male reproductive health may be threatened by environmental pollution, and it may be influence local population diathesis.

  7. Preliminary report on a novel straw semen freezing method for ram ...

    African Journals Online (AJOL)

    A straw (0.25ml French) semen freezing method for ram spermatozoa using a temperartre controlled stainless steel plate is described to possibly replace the conventional static liquid nitrogen vapour freezing method. 'n Strooitjie (0.25ml Franse) semenvriesmetode vir ramspermatosoa deur gebruikmaking van 'n ...

  8. HUBUNGAN ANTARA MOTILITAS DAN POLA PERGERAKAN SPERMATOZOA SEMEN SEGAR SAPI BALI JANTAN

    OpenAIRE

    MUHAMMAD, HUSNI

    2017-01-01

    2017 ABSTRAK MUHAMMAD HUSNI. I111 13 309. Hubungan Antara Motilitas dan Pola Pergerakan Spermatozoa Semen Segar Sapi Bali Jantan. Dibimbing oleh Muhammad Yusuf sebagai Pembimbing Utama dan Sudirman Baco sebagai Pembimbing Anggota. Penelitian ini bertujuan untuk megetahui pola pergerakan spermatozoa sapi Bali serta mengetahui hubungan antara motilitas spermatozoa dengan jarak tempuh dan kecepatan spermatozoaa sem...

  9. FREQUENCY OF ANEUPLOID SPERMATOZOA STUDIED BY MULTICOLOR FISH IN SERIAL SEMEN SAMPLES

    Science.gov (United States)

    Frequency of aneuploid spermatozoa studied by multicolor FISH in serial semen samplesM. Vozdova1, S. D. Perreault2, O. Rezacova1, D. Zudova1 , Z. Zudova3, S. G. Selevan4, J. Rubes1,51Veterinary Research Institute, Brno, Czech Republic; 2U.S. Environmental Protection A...

  10. Cryopreservation of Indian red jungle fowl (Gallus gallus murghi) semen with polyvinylpyrrolidone.

    Science.gov (United States)

    Rakha, Bushra Allah; Ansari, Muhammad Sajjad; Akhter, Shamim; Zafar, Zartasha; Hussain, Iftikhar; Santiago-Moreno, Julian; Blesbois, Elisabeth

    2017-10-01

    The Indian red jungle fowl is a sub-species of the genus Gallus native to South Asia; facing high risk of extinction in its native habitat. During cryopreservation, permeable cryoprotectants like glycerol are usually employed and we previously showed encouraging results with 20% glycerol. Because bird spermatozoa contain very little intracellular water, the possibility of replacing an internal cryoprotectant by an external one is opened. In the present study, we tested the replacement of internal cryoprotectant glycerol by the external cryoprotectant Polyvinylpyrrolidone (PVP). PVP is a non-permeable cryoprotectant and keeps the sperm in glassy state both in cooling and warming stages without making ice crystallization within the sperm cell. We evaluated the effect of various levels of polyvinylpyrrolidone (PVP) on Indian red jungle fowl semen quality and fertility outcomes. The qualifying semen ejaculates collected from eight mature cocks were pooled, divided into five aliquots, diluted (37 °C) with red fowl semen extender having PVP [0% (control) 4% (w/v), 6% (w/v), 8% (w/v) and 10% (w/v)]. Diluted semen was cryopreserved and stored in liquid nitrogen. The whole experiment was repeated/replicated for five times independently. Sperm motility, plasma membrane integrity, viability and acrosome integrity were recorded highest (P jungle fowl spermatozoa than glycerol and can be used in routine practice avoiding the contraceptive effects of glycerol. Copyright © 2017 Elsevier Inc. All rights reserved.

  11. Mechanisms of P-Glycoprotein Modulation by Semen Strychni Combined with Radix Paeoniae Alba

    Directory of Open Access Journals (Sweden)

    Li-Li Liu

    2017-01-01

    Full Text Available Semen Strychni has been extensively used as a Chinese herb, but its therapeutic window is narrowed by the strong toxicity of the compound, which limits its effectiveness. Radix Paeoniae Alba has been reported to reduce the toxic effects and increase the therapeutic effects of Semen Strychni, but the underlying mechanism remains unknown. This research aimed to explore the mechanism through which P-glycoprotein (P-gp is modulated by Semen Strychni combined with Radix Paeoniae Alba in vitro. An MTT assay was used to study cytotoxicity in an MDCK-MDR1 cell model. Rh123 efflux and accumulation were measured to assess P-gp function. The expression levels of MDR1 mRNA and P-gp protein in MDCK-MDR1 cells were investigated. A P-gp ATPase activity assay kit was applied to detect the effect on P-gp ATPase activity. Semen Strychni combined with Radix Paeoniae Alba could induce P-gp-mediated drug transport by inhibiting brucine and strychnine transport in MDCK-MDR1 cells, enhancing the P-gp efflux function, upregulating the P-gp expression and MDR1 mRNA levels, and stimulating P-gp ATPase activity.

  12. The effect of presence of facultative bacteria species on semen and ...

    African Journals Online (AJOL)

    Introduction: Infections of male urogenital tracts may contribute to male infertility. However, the effects of bacterial presence on sperm quality and fertility are controversial. Objectives: We investigated the occurrence of non-specific bacteria and quality/quantity of semen of infertile and fertile control groups in Nigeria. Subjects ...

  13. EVALUATION OF A COMPUTER-AIDED SEMEN ANALYSIS SYSTEM WITH SPERM TAIL DETECTION

    NARCIS (Netherlands)

    WIJCHMAN, JG; DEWOLF, BTHM; JAGER, S

    The aim of this study was to evaluate the Stroemberg-Mika cell motion analyser (SM-CMA) which uses tail detection in order to discriminate between immotile spermatozoa and other particles. Analysis of the spermatozoa by the SM-CMA can easily be checked on a video monitor. The semen samples were from

  14. Polymyxin B changes the plasma membrane integrity of cryopreserved bull semen

    Directory of Open Access Journals (Sweden)

    Mojtaba Rashedi

    2016-09-01

    Conclusion: Our study indicates that adding PMB to bull extender is efficient and appropriate, but, it must be mentioned that addictive amount will be due to extender composition, existence of other antibiotics and, importantly, the load of bacterial contamination in the semen.

  15. The effect of presence of facultative bacteria species on semen and ...

    African Journals Online (AJOL)

    C.A. Enwuru

    2016-08-01

    Aug 1, 2016 ... KEYWORDS. Facultative;. Bacteria;. Infertility;. Semen. Abstract. Introduction: Infections of male urogenital tracts may contribute to male infertility. However, the effects ... response with its concomitant oxidative stress. The effects of .... each species of facultative microbial organisms isolated in relation with the ...

  16. Relationship between self-reported residential indoor remodeling and semen quality: a case-control study

    Directory of Open Access Journals (Sweden)

    Mao-Hua Miao

    2015-01-01

    Full Text Available The present study examined the association between residential indoor remodeling and poor semen quality. Sperm donors aged 18-45 years old were recruited in Shanghai, China. Semen specimens were collected and analyzed. An in-person interview was conducted to obtain information on the history of indoor remodeling and potential confounders. A total of 70 participants with abnormal semen quality (case group and 68 controls were examined. A total of 20 subjects reported indoor remodeling in the recent 24 months, and among them 17 subjects reported indoor remodeling in the recent 12 months. Compared with participants with no history of indoor remodeling, participants with a history of indoor remodeling in the recent 24 months were more than three times as likely to have poor sperm quality (adjusted odds ratio = 3.8, 95% confidence interval: 1.3-12.0 after controlling for potential confounders. The association was strengthened when the analysis was restricted to those who had indoor remodeling in the recent 12 months. Our findings provide preliminary evidence that indoor remodeling has an adverse effect on semen quality.

  17. Effects of zinc on male sex hormones and semen quality in rats ...

    African Journals Online (AJOL)

    This study assessed the effects of zinc on male sex hormones and semen quality in male albino wistar rats. Forty rats weighing between 150- 210g, grouped into 5 of 8 rats each, were used for the research that lasted for six weeks. Group I, the control group, received normal rat chow and water ad libitum. The four test ...

  18. Revisiting the assessment of semen viscosity and its relationship to leucocytospermia.

    Science.gov (United States)

    Flint, M; du Plessis, S S; Menkveld, R

    2014-10-01

    With infertility challenges posing an obstacle to many couples, the extension of variables to assess male fertility is an important line of research. At the Reproductive Biology Unit where the study was undertaken, a considerable proportion of male patient's seeking fertility assessment presented with hyperviscous semen samples and elevated concentrations of leucocytes. Despite viscosity being included as part of a routine spermiogram, it raises a considerable amount of concern as it is assessed semiquantitatively. The study was undertaken to evaluate the quantification of semen viscosity in centipoise (cP) and to investigate whether a correlation exists between hyperviscosity and leucocytospermia. A total of 200 semen samples were assessed from a sample cohort of two population groups: 162 male patients undergoing fertility assessment and 38 volunteer donors. Semen viscosity was determined by measuring the filling time of a capillary-loaded Leja chamber and quantifying the viscosity in cP. Leucocytes were identified histochemically with a leucocyte peroxidase test. The viscosity when quantified in cP was significantly higher in the peroxidase positive sample group (9.01 ± 0.49 vs. 7.39 ± 0.23 cP; P viscosity may possibly help to identify, diagnose and treat patients suffering from leucocytospermia to ultimately enhance their fertility potential. © 2013 Blackwell Verlag GmbH.

  19. Effects of Ascorbic Acid on Garlic-induced Alterations in Semen ...

    African Journals Online (AJOL)

    Effects of Ascorbic Acid on Garlic-induced Alterations in Semen Parameters Of Wistar Rats. GO Omotoso, IO Onanuga, AAG Jimoh. Abstract. No Abstract. Full Text: EMAIL FULL TEXT EMAIL FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT · http://dx.doi.org/10.4314/tjhc.v18i2.69616 · AJOL African Journals ...

  20. Intralymphatic Immunotherapy With Autologous Semen in a Korean Man With Post-Orgasmic Illness Syndrome.

    Science.gov (United States)

    Kim, Tae Beom; Shim, Young Sup; Lee, Sang Min; Son, Eun Suk; Shim, Jung Woo; Lee, Sang Pyo

    2018-03-14

    Post-orgasmic illness syndrome (POIS) is a very rare disease characterized by local allergic symptoms and transient flu-like illness that nearly always occur after masturbation, coitus, or spontaneous ejaculation and last for 2 to 7 days. In a previous case report, 2 patients with POIS received hyposensitization therapy composed of multiple subcutaneous injections of autologous semen that resulted in a gradual decrease of symptoms. However, this procedure requires patients to endure pain and discomfort during frequent subcutaneous injections and preceding masturbations to obtain the autologous semen used for therapy. Recent studies have suggested that intralymphatic immunotherapy is a promising new method of allergen-specific immunotherapy against allergic diseases, showing a faster onset and longer duration of therapeutic effects after only several intralymphatic injections. We report on a case of a Korean man with POIS who received intralymphatic immunotherapy that alleviated POIS-related symptoms and in whom the existence of semen-specific immunoglobulin E was confirmed using immunoglobulin E immunoblotting and enzyme-linked immunosorbent assay. Kim TB, Shim YS, Lee, SM, et al. Intralymphatic Immunotherapy With Autologous Semen in a Korean Man With Post-Orgasmic Illness Syndrome. Sex Med 2018;X:XX-XX. Copyright © 2018. Published by Elsevier Inc.

  1. Gold in semen: Level in seminal plasma and spermatozoa of normal ...

    African Journals Online (AJOL)

    The study was conducted to understand the amount of gold in semen of normal and different infertile conditions. Gold was estimated in normal (n38) and pathological conditions (n86) by employing Atomic Absorption Spectrophotometer. Gold level observed in seminal plasma was as follows: in normozoospermia (n38) ...

  2. Gold in semen: Level in seminal plasma and spermatozoa of normal ...

    African Journals Online (AJOL)

    K.P. Skandhan

    2016-07-01

    Jul 1, 2016 ... Abstract The study was conducted to understand the amount of gold in semen of normal and dif- ferent infertile conditions. Gold was estimated in normal (n38) and pathological conditions (n86) by employing Atomic Absorption Spectrophotometer. Gold level observed in seminal plasma was as follows: in ...

  3. Effects of herbal preparation on libido and semen quality in boars.

    Science.gov (United States)

    Frydrychová, S; Opletal, L; Macáková, K; Lustyková, A; Rozkot, M; Lipenský, J

    2011-08-01

    The objective of this study was to investigate the effects of a preparation from herbal extracts (PHE) on libido and semen quality in breeding artificial insemination boars. Ten fertile boars were divided into control and experimental groups according to significant difference of libido. There were no differences in semen quality between groups. Animals were fed a commercial feeding mixture for boars. The feeding mixture for the experimental group was enriched with PHE, which was prepared from Eurycoma longifolia, Tribulus terrestris and Leuzea carthamoides. Duration of the experiment was 10 weeks. Samples of ejaculate were collected weekly. Libido was evaluated according to a scale of 0-5 points. Semen volume, sperm motility, percentage of viable spermatozoa, sperm concentration, morphologically abnormal spermatozoa, daily sperm production and sperm survival were assessed. Amounts of mineral components and free amino acids were analysed in seminal plasma. Significant differences were found in these parameters: libido (4.05 ± 0.22 vs 3.48 ± 0.78; p libido was increased by 20% in comparison with the beginning of the experiment. Results of this study showed positive effect of PHE on libido and some parameters of boar semen quality. © 2010 Blackwell Verlag GmbH.

  4. Effects of zinc on male sex hormones and semen quality in rats

    African Journals Online (AJOL)

    olayemitoyin

    Summary: This study assessed the effects of zinc on male sex hormones and semen quality in male albino wistar rats. Forty rats ... significant positive effects on male sex hormones and sperm quality at doses within physiological levels but harmful at higher doses. .... viability determination, while Evans and Walls stains.

  5. Cryobiology and a new look at the preservation of stallion semen

    DEFF Research Database (Denmark)

    Grout, Brian William Wilson; Lehn-Jensen, Henrik; Petersen, Morten Rønn

    2009-01-01

      During freeze-preservation of high-viability ejaculates of horse semen the duration of the equilibration time for nucleated straws (achieved at -7 0C following induced nucleation and during controlled, slow cooling to -60 0C) has little impact on viability, measured using propidium iodide...

  6. Effect of fructose addition in skim milk based extender on semen ...

    African Journals Online (AJOL)

    This experiment is designed to study the effect of fructose addition as energy source in skim milk extender on the quality fertility of white leghorn's spermatozoa stored at 4 °C. The treatments (T) in semen quality experiment were Tl (0% fructose + Modified Ringers Solution (MRS), T2 (5mM fructose + 1 % (w/v) skim milk ...

  7. Virulence and genotype of a bovine herpesvirus 1 isolate from semen of a subclinically infected bull

    NARCIS (Netherlands)

    Oirschot, van J.T.; Rijsewijk, F.A.M.; Straver, P.J.; Ruuls, R.C.; Quak, J.; Davidse, A.; Westenbrink, E.; Gielkens, A.L.J.; Dijk, van J.E.; Moerman, A.

    1995-01-01

    A bovine herpesvirus 1 (BHV-1) isolate from the semen of a subclinically infected bull was administered to cattle by various routes to assess its virulence. Cattle that were artificially inseminated or inoculated intrapreputially did not develop clinical signs, but did transmit the virus to contact

  8. Optimizing human semen cryopreservation by reducing test vial volume and repetitive test vial sampling

    DEFF Research Database (Denmark)

    Jensen, Christian F S; Ohl, Dana A; Parker, Walter R

    2015-01-01

    : Prospective clinical laboratory study. SETTING: University assisted reproductive technology (ART) laboratory. PATIENT(S): A total of 594 patients undergoing semen analysis and cryopreservation. INTERVENTION(S): Semen analysis, cryopreservation with different intermediate steps and in different volumes (50......-1,000 μL), and long-term storage in LN2 or VN2. MAIN OUTCOME MEASURE(S): Optimal TV volume, prediction of cryosurvival (CS) in ART procedure vials (ARTVs) with pre-freeze semen parameters and TV CS, post-thaw motility after two- or three-step semen cryopreservation and cryostorage in VN2 and LN2. RESULT......(S): Test vial volume of 50 μL yielded lower CS than other volumes tested. Cryosurvival of 100 μL was similar to that of larger volumes tested. An intermediate temperature exposure (-88°C to -93°C for 20 minutes) during cryopreservation did not affect post-thaw motility. Cryosurvival of TVs and ARTVs from...

  9. Cadmium may impair prostate function as measured by Prostate Specific Antigen in semen

    DEFF Research Database (Denmark)

    Andreucci, Alessandro; Mocevic, Emina; Jönsson, Bo A

    2015-01-01

    We investigated the association between cadmium in blood and the concentration of the prostate specific antigen (PSA) in semen, including the modifying effects of zinc or the CAG polymorphism in the androgen receptor (AR). Blood and semen samples were collected from 504 partners of pregnant women...... in Greenland, Poland and Ukraine. We found an inverse trend between cadmium and PSA (log(β) = -0.121, 95% confidence interval (CI): -0.213; -0.029, P = 0.0103) in Greenlandic men. Similar results were observed in men with a high number of CAG repeats (CAG 24) (log(β) = -0.231, 95% CI: -0.363; -0.098, P = 0.......0009). Inverse trends between cadmium and PSA were found when semen zinc concentrations were below the median value for men from Ukraine and Greenland. These outcomes suggest that cadmium may impair prostate function, as measured by PSA in semen, while high zinc levels and a low number of CAG repeats protects...

  10. A comparative evaluation of semen parameters in pre- and post-Hurricane Katrina human population

    Directory of Open Access Journals (Sweden)

    Caner Baran

    2015-01-01

    Full Text Available A natural disaster leading to accumulation of environmental contaminants may have substantial effects on the male reproductive system. Our aim was to compare and assess semen parameters in a normospermic population residing in the Southern Louisiana, USA area pre- and post-Hurricane Katrina. We retrospectively evaluated semen analyses data (n = 3452 of 1855 patients who attended the Tulane University Andrology/Fertility Clinic between 1999 and 2013. The study inclusion criteria were men whose semen analyses showed ≥ 1.5 ml volume; ≥15 million ml -1 sperm concentration; ≥39 million total sperm count; ≥40% motility; >30% morphology, with an abstinence interval of 2-7 days. After the inclusion criteria applied to the population, 367 normospermic patients were included in the study. Descriptive statistics and group-based analyses were performed to interpret the differences between the pre-Katrina (Group 1, 1999-2005 and the post-Katrina (Group 2, 2006-2013 populations. There were significant differences in motility, morphology, number of white blood cell, immature germ cell count, pH and presence of sperm agglutination, but surprisingly there were no significant differences in sperm count between the two populations. This long-term comparative analysis further documents that a major natural disaster with its accompanied environmental issues can influence certain semen parameters (e.g., motility and morphology and, by extension, fertility potential of the population of such areas.

  11. Increased prevalence of leukocytes and elevated cytokine levels in semen from Schistosoma haematobium-infected individuals

    DEFF Research Database (Denmark)

    Leutscher, Peter D C; Pedersen, Mette; Raharisolo, Clairette

    2005-01-01

    In this study, we investigated the seminal inflammatory response to egg infestation of the urogenital organs in 240 semen-donating men aged 15-49 years living in a Schistosoma haematobium-endemic area of Madagascar. In 29 subjects (12%) with excretion of > or =5 ova/ejaculate, leukocytospermia (>...

  12. Attempted semen collection using the massage technique in blue-fronted Amazon parrots (Amazona aestiva aestiva).

    Science.gov (United States)

    Della Volpe, Angelique; Volker, Schmidt; Krautwald-Junghanns, Maria-Elisabeth

    2011-03-01

    The purpose of this study was to establish a technique for collecting semen from blue-fronted Amazon parrots (Amazona aestiva aestiva) and to evaluate the samples that were collected. The massage method is the most common technique used to collect semen in birds and has been proven successful in several psittacine species; however, collection attempts in larger parrots have been unsatisfactory. Six blue-fronted Amazon parrot males, 3 paired with hens and 3 unpaired, were used in this study. The semen collection technique was revised to allow collection from individual birds by a single person. Semen collection was attempted from the 6 parrots on 52-56 occasions, which totaled 330 single attempts. Nineteen ejaculates were collected, and each bird produced at least 1 ejaculate that contained spermatozoa. Large ranges of sample volume (1-15.4 microL), sperm quality (motility = 2%-60%; live:dead ratio = 2:198 to 185:15), sperm concentration (0.79-3.3 x 10(6) sperm/mL), and contamination rate (0%-100%) were observed. Measured parameters did not appear to be significantly impacted by birds being paired or kept singly. Because of the relatively short acclimation period, the birds appeared to be sexually inactive for the majority of the study. Further research using sexually active birds will be necessary to determine standard spermatological parameters and verify the success of the methodology used here.

  13. Mycoplasma agalactiae in semen and milk of goat from Pernambuco state, Brazil

    Directory of Open Access Journals (Sweden)

    Bruno H.L.S. Alves

    2013-11-01

    Full Text Available In goat and sheep flocks, mycoplasmosis is a disease that may cause severe economical losses associated with polyarthritis, mastitis, agalactia, conjunctivitis, pneumonia and reproductive failure. The latter may involve repeat breeding, granular vulvovaginitis, infertility and abortions. The aim of the present study was to assess the occurrence of Mycoplasma agalactiae (Ma in semen and milk samples from naturally infected goat in the semiarid region from Pernambuco State, Northeast from Brazil. Thirty-nine semen samples and 81 milk samples were submitted to DNA extraction using a commercially available kit and following the manufacturer's instructions. The polymerase chain reaction (PCR was then performed in accordance with protocols described in the literature. The results of the present study revealed the presence of Ma in the DNA of 17.9% (7/39 of the semen samples and 3.7% (3/81 of the milk samples. The results obtained in the present study confirm the elimination of the DNA of Ma in the semen and milk samples. The presence of this agent in goat flocks is considered very risky in terms of reproductive disorders and contagious agalactia outbreaks in the Northeast region of Brazil.

  14. Physical and kinematic properties of cryopreserved camel sperm after elimination of semen viscosity by different techniques.

    Science.gov (United States)

    El-Bahrawy, Khalid; Rateb, Sherif; Khalifa, Marwa; Monaco, Davide; Lacalandra, Giovanni

    2017-12-01

    This investigation aimed to determine the influence of using different techniques for liquefaction of semen on post-thaw physical and dynamic characteristics of camel spermatozoa. A total of 144 ejaculates were collected from 3 adult camels, Camelus dromedarius, twice-weekly over 3 consecutive breeding seasons. A raw aliquot of each ejaculate was evaluated for physical and morphological properties, whereas the remaining portion was diluted (1:3) with glycerolated Tris lactose egg yolk extender, and was further subjected to one of the following liquefaction treatments: control (untreated), 5μl/ml α-amylase, 0.1mg/ml papain, 5u/ml bromelain, or 40-kHz nominal ultrasound frequency. The post-thaw objective assessment of cryopreserved spermatozoa, in all groups, was performed by a computer-assisted sperm analysis (CASA) system. The results revealed that all liquefaction treatments improved (P<0.05) post-thaw motility, viability and sperm motion criteria. However, an adverse effect (P<0.05) was observed in acrosome integrity, sperm cell membrane integrity and percent of normal sperm in all enzymatically-treated specimens compared to both control and ultrasound-treated semen. These results elucidate the efficiency of utilizing ultrasound technology for viscosity elimination of camel semen. In addition, developing enzymatic semen liquefaction techniques is imperious to benefit from when applying assisted reproductive technologies, particularly AI and IVF, in camels. Copyright © 2017 Elsevier B.V. All rights reserved.

  15. Cross Selling Implementation From Outpatient Unit to Radiology Unit in Semen Gresik Hospital

    OpenAIRE

    Rochmah, Thinni Nurul; Faradisa, Mutiara Ayu

    2013-01-01

    Thelow visitingnumberinhospital€™sunitarecloselyrelatedtomarketingactivities,includinginternal marketing which consistsof cross sellingfromother units.Thisstudy aims toanalyze crossselling implementation from Outpatient Unit to Radiology Unit in Semen Gresik Hospital. This study was a cross sectional analytic design. Samplewastakenbysimple random samplingwithsamplesize25respondents.Independentvariableswere marketing policy,employee commitment,perception, motivation,andreadiness ofcross sellin...

  16. Poor Acceptance Rate for Semen Donors to a Private Cryobank in ...

    African Journals Online (AJOL)

    Six (9.8%) of the donors were unable to masturbate and thus were disqualified, while only one donor (6.1%) failed to show up for a re-test after the completion of the quarantine period. Twenty one (38.2%) donors had low semen values based on the WHO reference value. We conclude that a high proportion of prospective ...

  17. Usulan Indikator Evaluasi Pemasok dalam Penetapan Bidder List: Studi Kasus Pengadaan Jasa PT. Semen Padang

    Directory of Open Access Journals (Sweden)

    Pri Gustari Akbar

    2016-04-01

    Full Text Available Each company will seek to improve performance in order to obtain the optimal results and customer satisfaction. One of the factors that affect the performance of the company is the supplier or vendor, which acts as a supplier of the operational needs of the company consisting of raw materials and spare parts manufacturing equipment. The existence of suppliers is crucial for the production process and the product to be produced. Therefore, it is necessary to a proper selection of suppliers so as to eliminate all risks that would result in losses for the company. PT. Semen Padang is one of the companies involved in the cement industry is one of the members of PT. Cement Indonesia as the Holding Company. Until now both PT. Semen Padang and PT. Cement Indonesia does not have yet a standard method in the selection of suppliers for the procurement process of goods and services. This study aims to design the proposed indicator in the evaluation of suppliers to determine the bidder's list on a tender for the procurement of services in PT. Semen Padang. Based on the literature study, it obtained six criteria i.e: quality, delivery, performance history, price, technical capability, and procedural compliance, which are divided into 13 indicators of performance evaluation. These indicators will be validated by the cement industry. Future studies will design a model of evaluation of suppliers using Analytic Hierarchy Process (AHP method to determine the bidder's list on a tender for the procurement of services in PT. Semen Padang.

  18. The limits for detection of activated caspases of spermatozoa by western blot in human semen.

    Science.gov (United States)

    Brugnon, F; Pons-Rejraji, H; Artonne, C; Janny, L; Grizard, G

    2012-08-01

    Detection of activated caspases of spermatozoa could be helpful to evaluate male infertility. Although western blot is validated as a highly specific method to detect the proteins extracted from cells, the ability of this technique to detect activated sperm caspases in human semen may be limited. Indeed, round cells, which potentially contain some activated caspases, may be present in semen and interfere with the detection of activated sperm caspases. Moreover, it is necessary to evaluate the minimum amount of spermatozoa necessary to optimise the detection of activated caspases in semen samples. Our results showed that interference due to round cells contained in semen with activated caspase-3 requires separation of spermatozoa by density migration. This sperm preparation selects a mature sperm population that does not reflect the whole sperm population, and in infertile men with oligoasthenoteratozoospermia, the amount of spermatozoa thus selected is usually low. Moreover, the western blot technique's low detection sensitivity and the low level of caspase enzyme activity in human spermatozoa for activated caspase-3, -8 and -9 mean that large quantities of spermatozoa are needed to detect the expression of the activated caspases. These limitations prevent this method being used for routine analysis in clinical practice. © 2012 Blackwell Verlag GmbH.

  19. Effects of pH during liquid storage of goat semen on sperm viability and fertilizing potential.

    Science.gov (United States)

    Liu, Chang-He; Dong, Hai-Bo; Ma, Dong-Li; Li, You-Wei; Han, Dong; Luo, Ming-Jiu; Chang, Zhong-Le; Tan, Jing-He

    2016-01-01

    A specific problem in goat semen preservation is the detrimental effect of seminal plasma on sperm viability in extenders containing yolk or milk. Thus, the use of chemically defined extenders will have obvious advantages. Although previous studies indicate that the initial pH of an extender is crucial to sustain high sperm motility, changes in extender pH during long-term semen storage have not been observed. Monitoring extender pH at different times of semen storage and modeling its variation according to nonlinear models is thus important for protocol optimization for long-term liquid semen preservation. The present results showed that during long-term liquid storage of goat semen, both sperm motility and semen pH decreased gradually, and a strong correlation was observed between the two. Whereas increasing the initial extender pH from 6.04 to 6.25 or storage with stabilized pH improved, storage with artificially lowered pH impaired sperm motility. Extender renewal improved sperm motility by maintaining a stable pH. Sperm coating with chicken (Gallus gallus) egg yolk improved motility by increasing tolerance to pH decline. A new extender (n-mZAP) with a higher buffering capacity was formulated, and n-mZAP maintained higher sperm motility, membrane integrity and acrosome intactness than the currently used mZAP extender did. Goat semen liquid-stored for 12 d in n-mZAP produced pregnancy and kidding rates similar to those obtained with freshly collected semen following artificial insemination. In conclusion, maintenance of a stable pH during liquid semen storage dramatically improved sperm viability and fertilizing potential. Copyright © 2015 Elsevier B.V. All rights reserved.

  20. Effects of cell phone use on semen parameters: Results from the MARHCS cohort study in Chongqing, China.

    Science.gov (United States)

    Zhang, Guowei; Yan, Huan; Chen, Qing; Liu, Kaijun; Ling, Xi; Sun, Lei; Zhou, Niya; Wang, Zhi; Zou, Peng; Wang, Xiaogang; Tan, Lu; Cui, Zhihong; Zhou, Ziyuan; Liu, Jinyi; Ao, Lin; Cao, Jia

    2016-05-01

    Epidemiological and experimental evidence for detrimental effects of cell phone use on semen quality is still equivocal. And that recruiting participants from infertility clinic not from general population may raise the possibility of a selection bias. To investigate effects of cell phone use on semen parameters in a general population,We screened and documented the cell phone use information of 794 young men from the Male Reproductive Health in Chongqing College students (MARHCS) cohort study in 2013, followed by 666 and 568 in 2014 and 2015, respectively. In the univariate regression analyses, we found that the daily duration of talking on the cell phone was significantly associated with decreased semen parameters, including sperm concentration [β coefficient=-6.32% per unit daily duration of talking on the cell phone (h); 95% confidence interval (CI), -11.94, -0.34] and total sperm count (-8.23; 95% CI, -14.38, -1.63) in 2013; semen volume (-8.37; 95% CI, -15.93, -0.13) and total sperm count (-16.59; 95% CI, -29.91, -0.73) in 2015]. Internet use via cellular networks was also associated with decreased sperm concentration and total sperm counts in 2013 and decreased semen volume in 2015. Multivariate analyses were used to adjust for the effects of potential confounders, and significant negative associations between internet use and semen parameters remained. Consistent but nonsignificant negative associations between talking on the cell phone and semen parameters persisted throughout the three study years, and the negative association was statistically significant in a mixed model that considered all three years of data on talking on the cell phone and semen quality. Our results showed that certain aspects of cell phone use may negatively affect sperm quality in men by decreasing the semen volume, sperm concentration, or sperm count, thus impairing male fertility. Copyright © 2016 Elsevier Ltd. All rights reserved.

  1. Keberhasilan IB menggunakan semen beku hasil sexing dengan metode sentrifugasi gradien densitas percoll (SGDP pada sapi Peranakan Ongole (PO

    Directory of Open Access Journals (Sweden)

    Moh Thoriq Fernanda

    2014-12-01

    Full Text Available The purpose of this study was to evaluate the implementation of Artificial Insemination using sexed frozen semen processed with percol density gradient sentrifugation method on service per conception (S/C, Non Return Rate (NRR and Conception Rate (CR of Ongole crossbred cattle. The material used in this study were 54 Ongole crossbred cattle which were purposively selected with criteria had conditions of estrus 2A+. Fifty five cattle were divided into 2 treatments such as P0 (non sexed semen with Post Thawing Motility (PTM quality 40% and P1 (sexed semen with PTM quality 5-10%. The study was conducted in the village Boro Bunut and Boro Kemantren Pakis subdistrict Malang. The study showed that P0 (non sexed semen had S/C 1.3, NRR0-21 74.07%, NRR22-42 74.07%, NRR43-63 74.07% and CR 74.07%, while P1 (sexed semen had S/C 1.5, NRR0-21 74.07%, NRR22-42 59.25%, NRR43-63 59.25% and CR 55.56%. These results indicated that P0 (non-sexed semen had better results than P1 (sexed semen, but statistically the two treatments were not significantly different (P>0.05. The highest percentage of cattle pregnancy (74.07% was found in P0 (non sexed semen as compared to P1 (sexed semen which was 59.25%. Keywords: Sexing, estrus, cattle

  2. Fertility results of artificial inseminations performed with liquid boar semen stored in X-cell vs BTS extender.

    Science.gov (United States)

    Haugan, T; Gaustad, A H; Reksen, O; Gröhn, Y T; Hofmo, P O

    2007-02-01

    The objective of the present field study was to compare the fertility results for boar semen diluted in X-cell stored up to 4-5 days before artificial insemination (AI) with semen diluted in Beltsville thawing solution (BTS) used for AI following 2-3 days of storage (where the first day being the collection day). A total number of 2601 double inseminations in Norwegian herds were included in this two-trial study. All the boars used in the study were mature cross-bred Norwegian Landrace x Duroc (LD), which were routinely used for AI in Norway. The inseminated gilts and sows were Norwegian Landrace x Yorkshire (LY). The AI doses contained 2.5 billion spermatozoa, and consisted of a mixture of semen from three, occasionally four, boars (i.e. heterospermic semen). Fertility was measured in terms of the likelihood of farrowing and subsequent litter size. The fertility of the semen in both of the extenders was satisfactory and no significant differences were found either in semen stored 4-5 days in X-cell compared with 2-3 days in BTS or in semen stored 2-3 days in X-cell compared with 2-3 days in BTS. The storage capability findings for the long-term extender X-cell could significantly simplify the practical issues of semen production and the distribution of AI doses containing 2.5 billion spermatozoa. However, in pig production systems where all semen is used within 2-3 days, the short-term extender BTS is as good as the more expensive extender X-cell.

  3. Lycopene Offers Protection against Oxidative Damage in Frozen-Thawn Bovine Semen

    Directory of Open Access Journals (Sweden)

    Eva Tvrdá

    2016-05-01

    Full Text Available Excessive amounts of reactive oxygen species (ROS associated with spermatozoa freezing and thawing cycles have the ability to cause oxidative stress, which may result in sperm membrane lipid peroxidation (LPO, DNA damage and apoptosis, leading to decreased sperm motility and fertilization ability. Lycopene, the most powerful singlet oxygen quencher of all carotenoids, may be a possible option for a more effective semen cryopreservation because of its antioxidant properties. By reacting with and neutralizing ROS, lycopene could reduce the incidence of oxidative stress and therefore, lessen the damage otherwise inflicted on frozen-thawn human or animal spermatozoa. This study focused to evaluate possible protective effects of lycopene on post-thawn bovine sperm and selected oxidative stress parameters. Ten bovine ejaculates were used in the study. Each ejaculate was splitted into two equal aliquots and diluted with a commercial semen extender containing lycopene (1.5 mM/L or no supplement (control, cooled to 4°C, frozen and kept in liquid nitrogen. Frozen straws were thawn in a water bath for subsequent experiments. Computer assisted semen analysis was used to evaluate spermatozoa motility, ROS generation was quantified using luminol-based luminometry and LPO was assessed via the TBARS assay and UV/VIS spectrophotometry. Lycopene supplementation to the semen extender significantly (P<0.001 increased the post-thawn spermatozoa motility (50.80±1.43% in comparison with the control (32.40±1.20%. Lycopene administration provided a significantly (P<0.001 higher protection against ROS overgeneration caused by semen freezing and thawing (17.80±2.82 RLU/sec/106 sperm when compared to the lycopene-free control (36.19±3.41 RLU/sec/106 sperm. It was determined that the presence of lycopene in the semen extender resulted in a significantly (P<0.001 lower lipid peroxidation of the spermatozoa membranes (16.43±0.56 µM/L in comparison to the control group

  4. Laktosa Mempertahankan Daya Hidup Spermatozoa Kambing Peranakan Etawah yang Dipreservasi dengan Plasma Semen Domba Priangan

    Directory of Open Access Journals (Sweden)

    Demianus Ferdinand Souhoka

    2009-09-01

    Full Text Available The purpose of this research was to examine the effect of lactose in Tris extender on theviability of Etawah crossbreed goat spermatozoa which is preserved in the plasma using Prianganram seminal plasma at 3–5oC. Semen was collected using artificial vagina once a week. Freshsemen was divided into four tubes, and centrifuged at 3,000 RPM for 30 min. Supernatant wasremoved and replaced which an equal volume with Priangan ram seminal plasma. Semen in firsttube was diluted with Tris extender-20% egg yolk without lactose (control. Semen in second,third, and fourth tubes were diluted with Tris extender-20% egg yolk and 0.3% (0.3 g per 100 mlextender (L0.3, 0.6% (L0.6, and 1.2% (L1.2 lactose, respectively. Diluted-semen were stored inrefrigerator at 3–5oC. Quality of diluted-semen including percentages of motile spermatozoa (MS,live spermatozoa (LS, and intact plasma membrane (IPM were evaluated daily during storage at3–5oC for four days. The data were analyzed by usi analysis of variance (ANOVA. Means werecompared significant difference test at 0.05 significant level. Results of this study showed thatmean volume, colour, consistency, pH, mass activity, spermatozoa concentration, MS, LS,spermatozoa abnormal, and IPM of Etawah crossbreed goat fresh semen were 0.68 ml, cream,thick, 7, ++/+++, 4,148.57 million cell/ml, 70%, 83.89%, 7.12%, and 84%, respectively. Addition oflactose in Tris extender could maintain the quality of semen during preservation. At day-5 ofstorage, the percentages of MS, LS, and IPM for treatment L0.6 (40, 55.6, and 53.8% weresignificantly (P<0.05 higher than treatment L1.2 (36, 50.2, and 51.8%, treatment L0.3 (34, 51.2,and 48.4%, and control (29, 41.4, and 41.8%. In conclusion, addition of 0.6% lactose in Trisextender is the best dose in maintaining the quality of Etawah crossbreed goat spermatozoa, andcould be maintaining percentage of motile spermatozoa 40% for four days after preserved at 3–5oC.

  5. Efecto del plasma seminal sobre el estado redox del semen equino criopreservado

    Directory of Open Access Journals (Sweden)

    Edison Pizarro L.

    2013-10-01

    Full Text Available Objetivo. Determinar el efecto del plasma seminal sobre la generación de especies reactivas de oxígeno (ERO y la peroxidación lipídica de semen equino criopreservado y su asociación con parámetros de calidad seminal. Materiales y métodos. El semen de cinco caballos de la raza criollo colombiano (dos eyaculados cada uno, fue criopreservado mediante un protocolo de congelación rápida, empleando un diluyente leche-yema de huevo, suplementado con 0%, 10% y 20% de plasma seminal equino. En muestras de semen fresco y criopreservado se evaluó la generación de ERO y la peroxidación lipídica por espectrofluorimetría, y los parámetros de calidad seminal de movilidad progresiva, vitalidad e integridad de membrana, mediante microscopia de contraste de fase. Para el análisis estadístico se ajustaron modelos mixtos y se realizaron análisis de regresión y correlación. Resultados. Se hallaron promedios post-descongelación de movilidad progresiva, vitalidad e integridad de membrana de 37.8%±20.2, 50.6% ± 14.6 y 37.8% ± 15.5, respectivamente. Para el semen fresco y criopreservado suplementado con 0%, 10% y 20% de plasma seminal, los promedios de producción de ERO (URF fueron de 13.34±10.7, 16.15 ± 13.5, 17.32 ± 16 y 22.98 ± 19.4, respectivamente; mostrando un incremento estadísticamente significativo (p≤0.05 en la producción de ERO por efecto de la criopreservación y la suplementación con plasma seminal. Los promedios de peroxidación lipídica (nmolMDA/ml para estos mismos tratamientos, fueron de 0.41 ± 0.25, 0.72±0.37, 0.51 ± 0.29 y 0.47±0.26, respectivamente; mostrando una reducción significativa (p≤0.05 de la peroxidación lipídica del semen suplementado con 10% y 20% de plasma seminal, respecto al semen no suplementado (0%. Conclusiones. El plasma seminal reduce la peroxidación lipídica del semen equino criopreservado.

  6. A data-driven search for semen-related phenotypes in conception delay.

    Science.gov (United States)

    Patel, C J; Sundaram, R; Buck Louis, G M

    2017-01-01

    Sperm count, morphology, and motility have been reported to be predictive of pregnancy, although with equivocal basis prompting some authors to question the prognostic value of semen analysis. To assess the utility of including semen quality data in predicting conception delay or requiring >6 cycles to become pregnant (referred to as conception delay), we utilized novel data-driven analytic techniques in a pre-conception cohort of couples prospectively followed up for time-to-pregnancy. The study cohort comprised 402 (80%) male partners who provided semen samples and had time-to-pregnancy information. Female partners used home pregnancy tests and recorded results in daily journals. Odds ratios (OR), false discovery rates, and 95% confidence intervals (CIs) for conception delay (time-to-pregnancy > 6 cycles) were estimated for 40 semen quality phenotypes comprising 35 semen quality endpoints and 5 closely related fecundity determinants (body mass index, time of contraception, lipids, cotinine and seminal white blood cells). Both traditional and strict sperm phenotype measures were associated with lower odds of conception delay. Specifically, for an increase in percent morphologically normal spermatozoa using traditional methods, we observed a 40% decrease in conception delay (OR = 0.6, 95% CI = 0.50, 0.81; p = 0.0003). Similarly, for an increase in strict criteria, we observed a 30% decrease in odds for conception delay (OR = 0.7, 95% CI = 0.52, 0.83; p = 0.001). On the other hand, an increase in percent coiled tail spermatozoa was associated with a 40% increase in the odds for conception delay (OR = 1.4, 95% CI = 1.12, 1.75; p = 0.003). However, our findings suggest that semen phenotypes have little predictive value of conception delay (area under the curve of 73%). In a multivariate model containing significant semen factors and traditional risk factors (i.e. age, body mass index, cotinine and ever having fathered a pregnancy), there was a

  7. Effects of the Seminal Plasma Iron and Lead Content on Semen Quality of Water Buffalo (Bubalus bubalis) Bulls

    OpenAIRE

    Mohammad-Hassan Khadem Ansari; Siamak Asri-Rezaei; Sayed Mortaza Alavi-Shoushtari; Mahdi Eghbali

    2010-01-01

    In order to determine iron and lead content of seminal plasma in water buffalo and to study their associations with the semen characteristics, 54 semen samples were collected from 10 buffalo bulls. The semen characteristics were evaluated; its iron and lead content were estimated by atomic absorption spectrophotometer. The iron and lead content of the seminal plasma (Mean ± SEM) was recorded as 40.68 ± 0.75 mg L-1 and 0.026 ± 0.008 mg L-1, respectively. The mean iron value was highly associat...

  8. Effects of diluents and plasma on honey bee (Apis mellifera L.) drone frozen-thawed semen fertility.

    Science.gov (United States)

    Gül, Aziz; Şahinler, Nuray; Onal, Ali G; Hopkins, Brandon K; Sheppard, Walter S

    2017-10-01

    Cryopreservation is an advanced method used to protect germplasm in liquid nitrogen. Honey bees are of special interest to protect because of their pollination activity and critical role in agriculture. There has been important progress in the cryopreservation of honey bee germplasm in recent years, leading to practical recovery of genetic material for breeding purposes following freezing. However, there remains room for improvement and the goal of the present study was to evaluate the effect of different "extenders" added post-thaw on the fertilization rate of cryopreserved honey bee semen. The purpose of adding extender post-thaw was to dilute the cryoprotectant to remove chemicals after centrifugation because of potential adverse effects. The control consisted of frozen-thawed semen without the addition of an extender; treatment groups included the addition of one of the following extenders: glucose solution, fresh ram semen plasma, fresh honey bee semen plasma, extender solution. All of the above treatments and frozen-thawed control were compared to fresh semen. For each group, 15 virgin queens were instrumentally inseminated with the semen-diluent solution and introduced into nucleus colonies to determine the brood patterns of the queens. Percentages of worker brood produced in the fresh semen, frozen-thawed semen control, glucose, fresh ram semen plasma, fresh honey bee semen plasma, and extender solution supplemented groups were 98.±1.1%, 47.0 ± 0.9%, 3.0 ± 0.8%, 0.3 ± 0.1%, 48.1 ± 4.1% and 40.3 ± 2.4%, respectively. Similiarly, spermatozoa numbers in the spermathecae of the same treatment groups were 3.6 × 10 6 , 1.6 × 10 6 , 7.3 × 10 5 , 4.7 × 10 5 , 8.1 × 10 5 , and 4.6 × 10 5 spermatozoa for the same treatment, respectively. The differences in both worker brood percentage and sperm count in the spermatheca were statistically significant (P drone semen plasma group. We found a positive correlation between sperm count in

  9. Contribution of semen trait selection, artificial insemination technique, and semen dose to the profitability of pig production systems: A simulation study.

    Science.gov (United States)

    Gonzalez-Pena, Dianelys; Knox, Robert V; Rodriguez-Zas, Sandra L

    2016-01-15

    The economic impact of selection for semen traits on pig production systems and potential interaction with artificial insemination (AI) technique and semen dose remains partially understood. The objectives of this study were to compare the financial indicators (gross return, net profit, cost) in a three-tier pig production system under one of two selection strategies: a traditional strategy including nine paternal and maternal traits (S9) and an advanced strategy that adds four semen traits (S13). Maternal traits included the number of pigs born alive, litter birth weight, adjusted 21-day litter weight, and the number of pigs at 21 days, and paternal traits included days to 113.5 kg, back fat, average daily gain, feed efficiency, and carcass lean percentage. The four semen traits included volume, concentration, progressive motility of spermatozoa, and abnormal spermatozoa. Simultaneously, the impact of two AI techniques and a range of fresh refrigerated semen doses including cervical AI with 3 × 10(9) (CAI3) and 2 × 10(9) (CAI2) sperm cells/dose, and intrauterine AI with 1.5 × 10(9) (IUI1.5), 0.75 × 10(9) (IUI0.75), and 0.5 × 10(9) (IUI0.5) sperm cells/dose were evaluated. These factors were also evaluated using a range of farrowing rates (60%-90%), litter sizes (8-14 live-born pigs), and a selected semen collection frequency. The financial impact of the factors was assessed through simulation of a three-way crossbreeding system (maternal nucleus lines A and B and paternal nucleus line C) using ZPLAN. The highest return on investment (profit/cost) of boars was observed at 2.33 collections/wk (three periods of 24 hours between collections). Under this schedule, a significant (P < 0.0001) interaction between the selection strategy and the AI technique-dose combination was identified for the gross return; meanwhile, significant (P < 0.0001) additive effects of the selection strategy and AI technique-dose combination were observed for the net

  10. Development of soya milk extender for semen cryopreservation of Karan Fries (crossbreed cattle).

    Science.gov (United States)

    Singh, V K; Singh, A K; Kumar, R; Atreja, S K

    2013-01-01

    Egg yolk based semen extenders are used widely, with the potential risk of xenobiotic contamination. This study was designed to develop a soya milk based extender to substitute egg yolk based extender for bovine semen cryopreservation. In the first experiment soya milk was prepared from fresh soya bean (Glycine max). Concentration of soya milk in tris based extender was standardized based on quality parameters of spermatozoa during liquid preservation at 5°C up to 72 h and compared with egg yolk tris (EYT) extender. Sperm in soya milk tris (SMT) extender with 25 percent soya milk showed no significant (P > 0.05) differences in all the quality parameters like motility, viability, membrane integrity and acrosome integrity, as compared to sperm in EYT extender up to 72h in liquid dilution. In the second experiment the Karan Fries semen was cryopreserved in SMT extender with 25 percent soya milk (selected from the first experiment) using different concentration of glycerol, as cryoprotectant, ranging from 6-7 percent with a difference of 0.2 percent to standardize optimum concentration based on post thaw motility of spermatozoa. Glycerol at a final concentration of 6.4 percent was found to be the best among all. Further, semen samples were split and cryopreserved in newly developed SMT extender containing 6.4 percent glycerol and compared with conventional EYT extender for post thaw sperm quality parameters and degree of cryocapacitation. There were no significant (P > 0.05) differences between sperm in EYT extender and SMT extender for post thaw motility, viability, membrane integrity, acrosome integrity and cryocapacitation. In conclusion, the newly developed SMT extender maintained comparable semen quality as compared to EYT extender hence it can.

  11. Fertility response of artificial insemination methods in sheep with fresh and frozen-thawed semen.

    Science.gov (United States)

    Masoudi, Reza; Zare Shahneh, Ahmad; Towhidi, Armin; Kohram, Hamid; Akbarisharif, Abbas; Sharafi, Mohsen

    2017-02-01

    The aim of this study was to evaluate the fertility response of artificial insemination (AI) methods with fresh and frozen sperm in sheep. In experiment 1, one hundred and fifty fat tailed Zandi ewes were assigned into 3 equal groups and inseminated with three AI methods consisting of vaginal, laparoscopic and trans-cervical AI with fresh semen. In experiment 2, a factorial study (3 AI methods × 2 extenders) was used to analyze the effects of three AI methods and two freezing extenders containing soybean lecithin (SL) or Egg yolk (EY) on reproductive performance of 300 fat tailed Zandi ewes. Also, total motility, progressive motility, viability and lipid peroxidation of semen were evaluated after freeze-thawing in two extenders. In result, there was no significant difference among three AI methods when fresh semen was used. In experiment 2, the highest percentage of pregnancy rate, parturition rate and lambing rate were obtained in laparoscopic AI group (P < 0.05). Although pregnancy rate, parturition rate and lambing rate in trans-cervical group were higher (P < 0.05) than vaginal group, the results were not as high as laparoscopic group. No difference was observed between SL and EY extenders and their performance was close to each other. It can be concluded that although no difference was observed on reproductive performance for fresh semen, trans-cervical AI was more efficient than vaginal method when frozen-thawed semen was used, but its efficiency was not as high as laparoscopic method. Also, SL extender can be an efficient alternative extender to preserve ram sperm during cryopreservation procedure without adverse effects of EY. Copyright © 2016. Published by Elsevier Inc.

  12. Broiler breeder semen quality as affected by trace minerals in vitro.

    Science.gov (United States)

    Barber, S J; Parker, H M; McDaniel, C D

    2005-01-01

    Research has shown that trace elements, such as Se, Mn, and Zn, can alter reproductive functions. The aim of the current study was to evaluate the sperm quality index (SQI) and sperm viability as affected by various levels and sources of Se, Mn, and Zn when added in vitro to broiler breeder semen. In vitro treatments consisted of the following sources and levels of minerals: Control, no minerals added to sperm; seleno L-methionine, 4 levels ranging from 8.78 to 7,896 microg/L; sodium selenite, 4 levels ranging from 8.78 to 7,896 microg/L; MnSO4, 8 levels ranging from 6,500 to 65,000 mg/L; Zn 180 (Zinpro Corporation), 4 levels ranging from 0.65 to 650 mg/L; and ZnSO4, 4 levels ranging from 0.65 to 650 mg/L. The addition of 7,896 microg of sodium selenite/L to semen was detrimental to sperm motility. Also, MnSO4 adversely affected SQI and sperm viability at concentrations of 6,500 mg/L and greater. Sperm viability was decreased when 650 mg/L of Zn 180 was added to semen. Sperm motility was depressed by exposure to Zn 180 at 650 mg/L and ZnSO4 at 65 and 650 mg/L. Our results suggest that these trace minerals must act at the reproductive tissue level during spermatogenesis to improve semen quality. Direct in vitro application of these elements to semen appears to be detrimental to spermatozoa.

  13. Effect of Fermented Cocoa Pod Husk Level in Concentrate on Etawah Grade Semen Quality

    Science.gov (United States)

    Munier, F. F.

    2018-02-01

    The purpose of study was to determine the effect of fermented CPH supplementation level in concentrate toward semen quality of Etawah Grade Buck (EGB). This research was conducted from August to October, 2011 at experiment pen and Feeds Science Laboratory, Faculty of Animal Science, Universitas Gadjah, Yogyakarta. Semen was collected from 4 heads of EGB with about 2 years old and weight approximately of 24 kg. Concentrate was main material of CPH fermentation with composition P0 0, P1 20%, P2 40% and P3 60% from total of ration. The composer was used by the Aspergillus niger BPT with density 9.1 × 107 of colony/g as many as 1% of CPH material (on dry matter). Semen collection was done every 1 time/week and to be done five times semen collection. An examination was consisting of volume, color, smell, consistency, and the pH. Meanwhile, for microscopic examination was motion of mass, individual, motility, viability of spermatozoa. pH of P0, P1, P2 and P3 were 6.95, 7.03, 7.50 and 7.79, respectively. Mass movement of P0, P1, P2 and P3 were +++, +++, ++ and ++, while viability of P0, P1, P2 and P3 were 79.57%, 79.47%, 59.97% and 51.13%, respectively. It was concluded that addition of CPH fermentation in concentrates up to 20% for of EGB has not affect to feed consumption but it has affect to decreasing of semen quality in both of microscopic and microscopic.

  14. Detection of sex hormone in serum and semen of patients with idiopathic oligospermia and its significance

    International Nuclear Information System (INIS)

    Wang Guohong; Xu Ruiji; Zhang Zhongshu; Wang Youji

    2005-01-01

    The objective of this study was to detect testosterone(T), free testosterone (FT) and sex hormone-binding globulin(SHBG) in serum and semen of patients with idiopathic oligospermia, and further analyze the relationship between T, FT, SHBG and idiopathic oligospermia. Blood and semen samples were collected from males of a normal control group and an idiopathic oligospermia group at 8:00-10:00 am. The sperm density in semen was detected by routine semen analysis, while T, FT, SHBG in serum and semen were detected by RIA. There were no significant differences in serum concentrations of T, FT, SHBG between normal control group [(30.03±13.07)nmol/L,(97.50±46.96)pmol/L, (40.37±16.73)nmol/L, respectively] and idiopathic oligspermia group [(28.11±11.54) nmol/L, (94.88±42.04) pmol/L, (41.61± 18.86)noml/LJ(all P>0. 05). There were significant differences in semsn concentrations of FT and SHBG between normal control group[(2.01±0.32) pmol/L, (0.17±0.21)nmol/L] and idiopathic oligspermia group [ (0.52±0.44) pmol/L, (0.22±0.15) nmol/LJ (P 0.05). Therefore, measurement of semsn FT, SHBG concentration could early reflect the function of tesis, which is useful for early diagnosis and treatment of idiopathic oligospermia. (authors)

  15. Use of cryotubes for the cryopreservation of tambaqui fish semen (Colossoma macropomum).

    Science.gov (United States)

    Maria, Alexandre Nizio; Carvalho, Allan Charles Marques; Araújo, Rafael Venâncio; Santos, Jadson Pinheiro; Carneiro, Paulo César Falanghe; Azevedo, Hymerson Costa

    2015-04-01

    Tambaqui (Colossoma macropomum) is a freshwater fish of great importance to aquaculture in several South American countries. Recent studies have developed a protocol for semen cryopreservation in 0.25 and 0.5 mL straws; however, this technique has limitations for fingerling production at a large scale due to the high fecundity of tambaqui. The main objective of this study was to evaluate the feasibility of using cryotubes (1.6 and 4.5 mL) for tambaqui semen cryopreservation. Semen samples were diluted in freezing solution (5% glucose solution, 10% methylglycol, 5% egg yolk), stored in 1.6 and 4.5 mL cryotubes, frozen in liquid nitrogen vapor at -175°C and transferred to a cryogenic container at -196°C. The cryotubes were thawed in a water bath at 60°C for 70 or 90 s and the motility (total motility - TM; progressive motility - PM; curvilinear velocity - VCL; straight line velocity - VSL and average path velocity - VAP) and the viability of sperm were evaluated. There was no significant difference in sperm motility and viability post-thawing between 1.6 and 4.5m L cryotubes, except for TM (47% and 40%, respectively). Thawing for 90 s provided better results, being used in fertilization trials. Although the fertilization rate did not differ between the cryotubes (41-45%), it was significantly lower than that for fresh semen (74%). A strong positive correlation was observed between the sperm motility and fertilization rate (r=0.69-0.89). We conclude that 1.6 and 4.5 mL cryotubes have high potential for tambaqui semen cryopreservation when thawed for a minimum time of 90 s at 60°C. Copyright © 2015 Elsevier Inc. All rights reserved.

  16. Successful artificial insemination in the koala (Phascolarctos cinereus) using extended and extended-chilled semen collected by electroejaculation.

    Science.gov (United States)

    Allen, Camryn D; Burridge, Michelle; Mulhall, Sarah; Chafer, Mandy L; Nicolson, Vere N; Pyne, Michael; Zee, Yeng Peng; Jago, Sophia C; Lundie-Jenkins, Geoff; Holt, William V; Carrick, Frank N; Curlewis, Jonathan D; Lisle, Allan T; Johnston, Stephen D

    2008-04-01

    Artificial insemination in the koala using chilled, electroejaculated semen provides for a marked improvement in the reproductive and genetic management of captive koala colonies in Australia and internationally, and makes available the option of using semen collected from wild populations to expand restricted gene pools. Dilution of koala semen for artificial insemination is complicated because koalas are induced ovulators, and it is thought that ovulating factors are present in the semen, so that semen extension for preservation purposes might be anticipated to result in a failure to induce ovulation. The first two experiments of this study were designed to determine whether artificial insemination using undiluted, extended, and extended-chilled semen collected by electroejaculation was capable of inducing a luteal phase and/or the production of pouch young. In Experiment 1, 1 ml undiluted electroejaculated semen, 2 ml diluted (1:1) semen, and 1 ml diluted (1:1) semen resulted in seven of nine, six of nine, and six of nine koalas showing a luteal phase, respectively; four pouch young were produced in each treatment. A second artificial insemination experiment was conducted in which 2 ml diluted (1:1) semen was administered in three groups of nine koalas. The first group received semen that had been collected and diluted immediately without chilling, the second group was deposited with semen stored chilled for 24 h, and the final group received semen that had been chilled for 72 h. In the first group, five females had a luteal phase, but none became pregnant. In group 2, two of the five females that had a luteal phase gave birth, whereas in group 3, four of the six females that had a luteal phase produced pouch young. In addition, experiment 3 was conducted to determine whether it was possible to produce pouch young by naturally mating koalas that were in the latter stages of their behavioral estrus; this information is important to the logistics of transporting

  17. Micoplasma hominis, Ureaplasma urealyticum y bacterias aeróbicas en el semen de hombres que consultan por infertilidad Micoplasma hominis, Ureaplasma urealyticum and aerobic bacteria present in the semen from men attending infertility service

    Directory of Open Access Journals (Sweden)

    Bertha Victoria Rodríguez Pendás

    2013-04-01

    Full Text Available Introducción: las infecciones en el semen humano pueden alterar la calidad espermática, y vincularse con problemas de infertilidad masculina. Objetivo: determinar la frecuencia de infecciones por Micoplasma hominis, Ureaplasma urealyticum y bacterias aeróbicas en el semen de hombres que consultan por infertilidad, e identificar si existe relación entre las infecciones encontradas y las alteraciones en las variables de calidad del semen. Métodos: se realizó un estudio descriptivo transversal, para evaluar muestras de semen de 140 hombres, con edades entre 20 y 45 años, provenientes de las consultas de infertilidad del Instituto Nacional de Endocrinología. Se realizó un espermograma completo, que incluyó leucocitospermia, siguiendo los lineamientos de la OMS, para determinar las variables cualitativas y cuantitativas del semen. Las muestras de semen fueron cultivadas en agar sangre y agar chocolate a 37° C en atmósfera de CO2 para investigar bacterias aeróbicas, y se utilizó un juego de reactivos (Mycoplasma System Plus que permite realizar el cultivo, la identificación, el conteo semicuantitativo y el antibiograma de micoplasmas/ureaplasma urogenitales. Se tuvo en cuenta los aspectos éticos, y los resultados obtenidos se analizaron mediante cálculo de por cientos y la aplicación de la prueba de chi cuadrado. Resultados: de las 140 muestras de semen evaluadas, 58 (41,4 % mostraron la presencia de infecciones, de ellas 37 correspondieron a Ureaplasma urealyticum (25,7 %, 2 a Micoplasma hominis (1,4 % y 19 a bacterias aeróbicas (13,8 %. Al comparar las variables cualitativas y cuantitativas del semen con los sujetos infectados y no infectados, no se observaron diferencias estadísticamente significativas en ninguna de las variables de calidad espermática evaluadas. Conclusiones: la frecuencia total de infecciones, en la muestra estudiada, fue relativamente alta, pero no asociada a alteraciones en las variables seminales

  18. [Expedition medicine].

    Science.gov (United States)

    Donlagić, Lana

    2009-01-01

    Expedition and wildeness medicine is a term that combines rescue medicine, sport medicine as well as more specific branches as polar or high altitude medicine. It is being intensively studied both at the reaserch institutes and on expeditions. Ophtalmologists are concentrated on the reaserch of HARH (High Altitude Retinal Hemorrhage), neurologists on HACE reaserch (High Altitude Cerebral Edema), psychologists are developing tests to decsribe cognitive functions and many physicians are being trained to work in extreme enviroment. The result of all this effort are numerous new findings in pathophysiology and therapy of altitude illness, increased security on expedition and further development of expeditionism.

  19. Nuclear medicine

    International Nuclear Information System (INIS)

    Anon.

    1993-01-01

    The area of nuclear medicine, the development of artificially produced radioactive isotopes for medical applications, is relatively recent. Among the subjects covered in a lengthy discussion are the following: history of development; impact of nuclear medicine; understanding the most effective use of radioisotopes; most significant uses of nuclear medicine radioimmunoassays; description of equipment designed for use in the field of nuclear medicine (counters, scanning system, display systems, gamma camera); description of radioisotopes used and their purposes; quality control. Numerous historical photographs are included. 52 refs

  20. Sociodemographic characteristics and attitudes towards motherhood among single women compared with cohabiting women treated with donor semen

    DEFF Research Database (Denmark)

    Salomon, Maria; Sylvest, Randi; Hansson, Helena

    2015-01-01

    OBJECTIVE: To examine sociodemographic characteristics, family backgrounds, reproductive histories, and attitudes towards motherhood in single vs. cohabiting women seeking treatment with donor semen. DESIGN: Baseline data collection in a multicenter cohort study. SETTING: All nine public fertilit...

  1. Human semen quality in the new millennium: a prospective cross-sectional population-based study of 4867 men

    DEFF Research Database (Denmark)

    Jørgensen, N; Joensen, Ulla Nordström; Jensen, TK

    2012-01-01

    sperm concentration and sperm morphology. Comparing with historic data of men attending a Copenhagen infertility clinic in the 1940s and men who recently became fathers, these two groups had significantly better semen quality than our study group from the general population. Over the 15 years, median......Objectives Considerable interest and controversy over a possible decline in semen quality during the 20th century raised concern that semen quality could have reached a critically low level where it might affect human reproduction. The authors therefore initiated a study to assess reproductive...... health in men from the general population and to monitor changes in semen quality over time. Design Cross-sectional study of men from the general Danish population. Inclusion criteria were place of residence in the Copenhagen area, and both the man and his mother being born and raised in Denmark. Men...

  2. Hubungan antara pola konsumsi protein dan Fe dengan daya tahan jantung paru atlet sepakbola PS Semen Padang tahun 2003

    Directory of Open Access Journals (Sweden)

    Kusumawati M

    2005-07-01

    Conclusion: Protein and Fe consumption patterns infl uenced the cardiorespiratory endurance athlete PS.Semen Padang through hemoglobin rate cause of hemoglobin rate have signifycant relation with the cardiorespiratory endurance.

  3. Effect of Addition of Concentrated Proteins and Seminal Plasma Low Molecular Weight Proteins in Freezing and Thawing of Equine Semen

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    Fagundes, B.

    2011-07-01

    Full Text Available Difficulties in obtaining equine frozen semen with potential fertility are recognized. This study was designed to investigate the effect of seminal plasma on frozen/thawing of eight stallion semen from different breed using the following treatments: Seminal plasma with ten-fold concentrated proteins with molecular weight above 10 kDa on frozen extender; Part of seminal plasma with proteins under 10 kDa on frozen extender; Conventional freezing, using whole seminal plasma on frozen extender. Using the parameter of 30% of seminal motility post-thawing as index of good freezability, it was verified an increased percentage of stallions that presented good freezability when semen was frozen with seminal plasma containing ten-fold concentrated proteins with molecular weight above 10 kDa on frozen extender. These results, suggested the use of seminal plasma concentrated proteins from own stallion to freezing/thawing semen.

  4. Effect of platelet activating factor (PAF) supplementation in semen extender on viability and ATP content of cryopreserved canine spermatozoa.

    Science.gov (United States)

    Kordan, W; Lecewicz, M; Strzezek, R; Dziekońska, A; Fraser, L

    2010-01-01

    The aim of this study was to investigate the effect of platelet activating factor (PAF) on the quality characteristics of cryopreserved canine spermatozoa. Cryopreserved semen of 5 mixed-breed dogs was treated with different concentrations of exogenous PAF (1 x 10(-3) M, 1 x 10(-4) M, 1 x 10(-5) M and 1 x 10(-6) M) and examined at different time intervals (0, 30, 60 and 120 min). Cryopreserved semen treated without PAF was used as the control. Sperm quality was evaluated for motility (computer-assisted semen analysis, CASA), mitochondrial function (JC-1/PI assay) and plasma membrane integrity (SYBR-14/PI assay and Hoechst 33258). Also, ATP content of spermatozoa was determined using a bioluminescence assay. Treatment of cryopreserved semen with 1 x 10(-3) M PAF at 120 min of incubation resulted in significantly higher total sperm motility compared with the control. It was observed that PAF-improved total sperm motility was concurrent with enhanced sperm motility patterns after treatment of cryopreserved semen. Treatment of cryopreserved semen with PAF did not improve either sperm mitochondrial function or plasma membrane integrity, as monitored by different fluorescent membrane markers. Furthermore, ATP content of cryopreserved spermatozoa was significantly higher when PAF was used at a concentration of 1 x 10(-3) M compared with the control and other PAF treatments, regardless of the incubation time. The findings of this study indicated that treatment with 1 x 10(-3) M PAF at 120 min of incubation rendered better quality of cryopreserved canine semen, which was associated with improved sperm motility parameters and ATP content. It can be suggested that exogenous PAF addition is beneficial as a supplement for canine semen extender used for cryopreservation.

  5. Effect of season and age on Indian red jungle fowl (Gallus gallus murghi) semen characteristics: A 4-year retrospective study.

    Science.gov (United States)

    Rakha, B A; Ansari, M S; Akhter, S; Blesbois, E

    2017-09-01

    The reproductive potential of the adult males is expected to vary with age/season and largely differ not only in closely related avian species but even in subspecies, breeds and/or strains of the same species. Thus, it is pre-requisite to have knowledge of seminal parameters to achieve maximum production potential of at-risk species for ex situ in vitro conservation programs. A 4-year study was designed to evaluate the effect of age and season (spring, summer, autumn and winter) on semen characteristics of Indian red jungle fowl (Gallus gallus murghi) in a retrospective manner. Semen ejaculates (n = 1148) were regularly collected from eight adult cocks 6 to 54 months of age. Quantitative and qualitative semen parameters viz; volume (μL), concentration (1 × 10 9  mL -1 ), total sperm number per ejaculate (1 × 10 9  mL -1 ), motility (%), viability (%), plasma membrane integrity (%), acrosome integrity (%) and semen quality factor were recorded. A chronological increasing trend with age of most sperm quantitative and qualitative traits (semen volume, sperm concentration, total sperm number per ejaculate, plasma membrane integrity, viability, acrosomal integrity and semen quality factor) was observed. The highest values were observed at four years of age (P jungle fowl exhibits an evolution of sperm production that greatly differs in many points from other fowl sub-species. It is suggested that semen ejaculates of highest quality achieved for semen banking at the age of four year in the spring season. Copyright © 2017 Elsevier Inc. All rights reserved.

  6. The effects of frequent electroejaculation on the semen characteristics of a captive Siberian tiger (Panthera tigris altaica)

    OpenAIRE

    FUKUI, Daisuke; NAGANO, Masashi; NAKAMURA, Ryohei; BANDO, Gen; NAKATA, Shinichi; KOSUGE, Masao; SAKAMOTO, Hideyuki; MATSUI, Motozumi; YANAGAWA, Yojiro; TAKAHASHI, Yoshiyuki

    2013-01-01

    Artificial insemination (AI) can help to avoid inbreeding and genetic degeneration for sustaining genetically healthy populations of endangered species in captivity. Collection of a sufficient quantity of viable sperm is an essential first step in the AI process. In the present study, we examined the effects of frequent electroejaculation on semen characteristics in a Siberian tiger. We collected semen in all 17 trials during 6 breeding seasons (6 years). The mean number of sperm and the perc...

  7. Inverse U-shaped Association between Sleep Duration and Semen Quality: Longitudinal Observational Study (MARHCS) in Chongqing, China.

    Science.gov (United States)

    Chen, Qing; Yang, Huan; Zhou, Niya; Sun, Lei; Bao, Huaqiong; Tan, Lu; Chen, Hongqiang; Ling, Xi; Zhang, Guowei; Huang, Linping; Li, Lianbing; Ma, Mingfu; Yang, Hao; Wang, Xiaogang; Zou, Peng; Peng, Kaige; Liu, Taixiu; Cui, Zhihong; Ao, Lin; Roenneberg, Till; Zhou, Ziyuan; Cao, Jia

    2016-01-01

    To investigate the association between sleep duration and semen parameters as well as reproductive hormone levels. We designed a cohort of male college students in Chongqing, China. A total of 796 subjects were recruited in 2013 and 656 (82.4%) were followed up in 2014. Each time, semen and peripheral blood samples were collected for semen quality and reproductive hormone measurement. Sleep duration was estimated by revised Munich Chronotype Questionnaire. In 2014, sleep quality was also measured by Pittsburgh Sleep Quality Index (PSQI). There was a substantial inverse U-shaped association between sleep duration and two semen parameters (semen volume and total sperm number), with 7.0-7.5 h/day of sleep showing highest parameters. Either longer or shorter sleep was associated with decreased semen parameters in a dose-response manner (P = 0.002 and 0.001, respectively). Sleeping > 9.0 h was associated with a 21.5% (95% confidence interval 9.2, 32.2) reduction in semen volume and 39.4% (23.3, 52.1) reduction in total sperm number; sleeping ≤ 6.5 h was associated with 4.6% (-10.5, 22.3) and 25.7% (-1.2, 60.1) reduction. Increase of the two parameters was found in those who changed sleep duration toward 7.0-7.5 h/day from 2013 to 2014. The U-shaped association was independent from PSQI and was replicated in another dataset of 1,346 males. No association found between sleep duration and reproductive hormone. Either restricted or excessive sleep may impair semen quality. Further research is needed to validate this finding. © 2016 Associated Professional Sleep Societies, LLC.

  8. Tiempo de latencia para semen colectado de Colossoma macropomum “Gamitana” en solución sacarosa

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    Ehrlich Llasaca-Calizaya

    2014-12-01

    Full Text Available El objetivo fue estimar el tiempo de latencia (almacenamiento, para el semen de Colossoma macropomum, “gamitana” en solución de 400 mM de Sacarosa. Se consideró aceptable los niveles de motilidad superiores al 40%, lo cual garantiza eficientes tasas de fertilización. Para el desarrollo del experimento se colectó 2 lotes de semen inmótiles de gamitana (inducidos con Conceptal®, los cuales posteriormente fueron activados con agua destilada. El primer lote estuvo constituido por semen en sacarosa 400 mM, puro, a temperatura ambiente y refrigerado (4°C. La motilidad fue evaluada, cada hora, hasta la 7ma hora post colecta. El segundo lote con un semen en sacarosa 400mM a temperatura refrigerada y evaluada cada 12 horas. Los resultados del primer lote de semen demuestran que a partir de la 7ma hora hacia delante los índices de motilidad caen significativamente por debajo del 40%. Los resultados del segundo lote demuestran la viabilidad de utilizar solución de sacarosa, como medio de conservación, para mantener semen refrigerado por 2 días y activarlos con agua destilada. El proceso de extraer y colocar repetidas veces la misma muestra en refrigeración, limita el tiempo de viabilidad de semen con sacarosa en 8 horas aproximadamente. La utilización de sacarosa como medio para almacenar semen inmotil viable de gamitana, ayuda a conservar los espermatozoides por tiempos relativamente cortos.

  9. Tiempo de latencia para semen colectado de Colossoma macropomum “Gamitana” en solución sacarosa

    Directory of Open Access Journals (Sweden)

    Ehrlich Llasaca-Calizaya

    2015-01-01

    Full Text Available El objetivo fue estimar el tiempo de latencia (almacenamiento, para el semen de Colossoma macropomum, “gamitana” en solución de 400 mM de Sacarosa. Se consideró aceptable los niveles de motilidad superiores al 40%, lo cual garantiza eficientes tasas de fertilización. Para el desarrollo del experimento se colectó 2 lotes de semen inmótiles de gamitana (inducidos con Conceptal®, los cuales posteriormente fueron activados con agua destilada. El primer lote estuvo constituido por semen en sacarosa 400 mM, puro, a temperatura ambiente y refrigerado (4°C. La motilidad fue evaluada, cada hora, hasta la 7ma hora post colecta. El segundo lote con un semen en sacarosa 400mM a temperatura refrigerada y evaluada cada 12 horas. Los resultados del primer lote de semen demuestran que a partir de la 7ma hora hacia delante los índices de motilidad caen significativamente por debajo del 40%. Los resultados del segundo lote demuestran la viabilidad de utilizar solución de sacarosa, como medio de conservación, para mantener semen refrigerado por 2 días y activarlos con agua destilada. El proceso de extraer y colocar repetidas veces la misma muestra en refrigeración, limita el tiempo de viabilidad de semen con sacarosa en 8 horas aproximadamente. La utilización de sacarosa como medio para almacenar semen inmotil viable de gamitana, ayuda a conservar los espermatozoides por tiempos relativamente cortos.

  10. DNA methylation profiling for a confirmatory test for blood, saliva, semen, vaginal fluid and menstrual blood.

    Science.gov (United States)

    Lee, Hwan Young; Jung, Sang-Eun; Lee, Eun Hee; Yang, Woo Ick; Shin, Kyoung-Jin

    2016-09-01

    The ability to predict the type of tissues or cells from molecular profiles of crime scene samples has important practical implications in forensics. A previously reported multiplex assay using DNA methylation markers could only discriminate between 4 types of body fluids: blood, saliva, semen, and the body fluid which originates from female reproductive organ. In the present study, we selected 15 menstrual blood-specific CpG marker candidates based on analysis of 12 genome-wide DNA methylation profiles of vaginal fluid and menstrual blood. The menstrual blood-specificity of the candidate markers was confirmed by comparison with HumanMethylation450 BeadChip array data obtained for 58 samples including 12 blood, 12 saliva, 12 semen, 3 vaginal fluid, and 19 skin epidermis samples. Among 15CpG marker candidates, 3 were located in the promoter region of the SLC26A10 gene, and 2 of them (cg09696411 and cg18069290) showed high menstrual blood specificity. DNA methylation at the 2CpG markers was further tested by targeted bisulfite sequencing of 461 additional samples including 49 blood, 52 saliva, 34 semen, 125 vaginal fluid, and 201 menstrual blood. Because the 2 markers showed menstrual blood-specific methylation patterns, we modified our previous multiplex methylation SNaPshot reaction to include these 2 markers. In addition, a blood marker cg01543184 with cross reactivity to semen was replaced with cg08792630, and a semen-specific unmethylation marker cg17621389 was removed. The resultant multiplex methylation SNaPshot allowed positive identification of blood, saliva, semen, vaginal fluid and menstrual blood using the 9CpG markers which show a methylation signal only in the target body fluids. Because of the complexity in cell composition, menstrual bloods produced DNA methylation profiles that vary with menstrual cycle and sample collection methods, which are expected to provide more insight into forensic menstrual blood test. Moreover, because the developed

  11. Effect of egg yolk powder on freezability of Murrah buffalo (Bubalus bubalis semen

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    N. Kumar

    2016-06-01

    Full Text Available Aim: The aim of this study was to investigate the effect of commercial egg yolk powder as an alternative to fresh egg yolk on freezability of Murrah buffalo semen. Materials and Methods: Semen samples (12 from 3 Murrah buffaloes (4 from each bull with mass motility (≥3+ and total motility (70% and above were utilized in this study. Immediately after collection, each sample was divided into four groups. Groups I was diluted up to 60×106 sperm/ml with tris extender containing 10% fresh egg yolk and Groups II, III, and IV were diluted up to 60×106 sperm/ml with tris extender containing 2%, 4%, and 6% egg yolk powder, respectively. Semen samples were processed and cryopreserved followed by examination of frozen semen samples after 24 h. Semen samples from each group were evaluated for total motility, viability, acrosomal integrity, abnormality, and hypo-osmotic swelling test (HOST response after dilution, pre-freeze, and post-thaw stage. Results: Pre-freeze total motility was significantly (p<0.05 higher in Groups III and IV as compared to Groups I and II, and post-thaw total motility was significantly (p<0.01 higher in Group III as compared to other three groups. Viability was significantly (p<0.05 higher in Groups II, III, and IV than Group I at the pre-freeze stage. Significantly (p<0.01 higher viability and acrosomal integrity were recorded in Group III as compared to other three groups at the post-thaw stage. Abnormality was significantly (p<0.05 higher in Group IV than other three groups. HOST response was significantly (p<0.05 higher in Groups II and III than Groups I and IV at the pre-freeze and post-thaw stages. Conclusion: Addition of egg yolk powder at 4% level yielded significantly better results in terms of post-thaw semen quality as compared to the fresh egg yolk and other concentrations of egg yolk powder (2% and 6%.

  12. Mitochondrial Biomarkers Reflect Semen Quality: Results from the MARCHS Study in Chongqing, China.

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    Guowei Zhang

    Full Text Available Unexplained infertility requires that more sensitive and mechanism-based biomarkers should be developed and used independently of or in addition to conventional semen parameters for an infertility diagnosis. In the present study, semen samples were collected from young men participating in the Male Reproductive Health in Chongqing College students (MARCHS cohort study in the follow-up stage in 2014. Conventional semen parameters were measured in all 656 participants, whereas sperm mitochondrial membrane potential (MMP, mitochondrial DNA copy number (mtDNAcn, mtDNA integrity and apoptotic parameters were measured among 627, 386, 362, and 628 participants, respectively. We found that sperm MMP was significantly positively correlated with all of conventional semen parameters including semen volume (r = 0.090, p = 0.025, sperm concentration (r = 0.301, p<0.01, total sperm count (r = 0.324, p<0.01, and progressive motility (r = 0.399, p<0.01; sperm MMP was also negatively correlated with Annexin V+ sperm (r = -0.553, p<0.01; mtDNAcn was significantly negatively correlated with sperm concentration (r = -0.214, p<0.01, total sperm count (r = -0.232, p<0.01, and progressive motility (r = -0.164, p = 0.01; mtDNA integrity was also significantly positively correlated with sperm concentration (r = 0.195, p<0.01, total sperm count (r = 0.185, p<0.01, and progressive motility (r = 0.106, p = 0.043. After adjusting for potential confounders, these relationships remained significant. Furthermore, we explored the potential effects of lifestyles on such mitochondrial biomarkers and found that the current drinkers displayed a higher level of sperm MMP; additionally, mt DNAcn was increased with age. The results indicated that certain mitochondrial biomarkers could serve as predictors of semen quality in a general population, and the study provides a baseline for the effects of population characteristics and lifestyles on such mitochondrial markers.

  13. Lifestyles Associated With Human Semen Quality: Results From MARHCS Cohort Study in Chongqing, China.

    Science.gov (United States)

    Yang, Huan; Chen, Qing; Zhou, Niya; Sun, Lei; Bao, Huaqiong; Tan, Lu; Chen, Hongqiang; Zhang, Guowei; Ling, Xi; Huang, Linping; Li, Lianbing; Ma, Mingfu; Yang, Hao; Wang, Xiaogang; Zou, Peng; Peng, Kaige; Liu, Kaijun; Liu, Taixiu; Cui, Zhihong; Liu, Jinyi; Ao, Lin; Zhou, Ziyuan; Cao, Jia

    2015-07-01

    Decline of semen quality in past decades is suggested to be potentially associated with environmental and sociopsychobehavioral factors, but data from population-based cohort studies is limited. The male reproductive health in Chongqing College students (MARHCS) study was established in June 2013 as a perspective cohort study that recruited voluntary male healthy college students from 3 universities in Chongqing. The primary objectives of the MARHCS study are to investigate the associations of male reproductive health in young adults with sociopsychobehavioral factors, as well as changes of environmental exposure due to the relocation from rural campus (in University Town) to metro-campus (in central downtown). A 93-item questionnaire was used to collect sociopsychobehavioral information in manner of interviewer-interviewing, and blood, urine and semen samples were collected at the same time. The study was initiated with 796 healthy young men screened from 872 participants, with a median age of 20. About 81.8% of this population met the WHO 2010 criteria on semen quality given to the 6 routine parameters. Decreases of 12.7%, 19.8%, and 17.0%, and decreases of 7.7%, 17.6%, and 14.7% in total sperm count and sperm concentration, respectively, were found to be associated with the tertiles of accumulated smoking amount. Fried food consumption (1-2  times/wk or ≥3  times/wk vs nonconsumers) was found to be associated with decreased total sperm count (10.2% or 24.5%) and sperm concentration (13.7% or 17.2%), respectively. Coffee consumption was found to be associated with increased progressive and nonprogressive motility of 8.9% or 15.4% for subjects consuming 1-2  cups/wk or ≥3  cups/wk of coffee, respectively. Cola consumption appeared an association with decreased semen volume at 4.1% or 12.5% for 1-2  bottles/wk or ≥3  bottles/wk. A cohort to investigate the effects of environmental/sociopsychobehavioral factors act on semen quality was successfully

  14. Vulnerable Medicine

    Science.gov (United States)

    Bochner, Arthur P.

    2009-01-01

    In "Narrative Medicine: Honoring the Stories of Illness," Rita Charon paints an original and humane portrait of what it can mean to be a doctor, to live a life immersed in sickness and dedicated to wellness. Charon drops the veil, inviting readers to look at the secret, subjective, emotional face of medicine, a zone of self-censored feelings and…

  15. Prophylactic administration of an extract from Plantaginis Semen and its major component aucubin inhibits mechanical allodynia caused by paclitaxel in mice.

    Science.gov (United States)

    Andoh, Tsugunobu; Kato, Mitsuru; Kitamura, Ryo; Mizoguchi, Shizuka; Uta, Daisuke; Toume, Kazufumi; Komatsu, Katsuko; Kuraishi, Yasushi

    2016-07-01

    The chemotherapeutic agent paclitaxel (PTX) causes peripheral neuropathy as a major dose-limiting side effect, and this peripheral neuropathy is difficult to control. Our previous report showed that prophylactic repetitive administration of goshajinkigan ( niú chē shèn qì wán), but not hachimijiogan ( bā wèi dì huáng wán), which lacks two of the constituents of goshajinkigan, inhibited PTX-induced mechanical allodynia in mice. Thus, the herbal medicines Plantaginis Semen ( chē qián zǐ) or Achyranthis Radix ( niú xī) may contribute to the inhibitory action of goshajinkigan on the exacerbation of PTX-induced mechanical allodynia [Andoh et al, J. Tradit. Complement. Med. 2014; 4: 293-297]. Therefore, in this study, we examined whether an extract of Plantaginis Semen (EPS) or Achyranthis Radix (EAR) would relieve PTX-induced mechanical allodynia in mice. A single intraperitoneal injection of PTX caused mechanical allodynia, which peaked on day 14 after injection. Repetitive oral administration of EPS, but not EAR, starting from the day after PTX injection significantly inhibited the exacerbation of PTX-induced mechanical allodynia. Repetitive intraperitoneal injection of aucubin, one of the main components of EPS, starting from the day after PTX injection also significantly reduced PTX-induced mechanical allodynia. However, repetitive intraperitoneal injection of geniposide acid (a precursor of aucubin) or catalpol (a metabolite of aucubin) did not prevent the exacerbation of mechanical allodynia. These results suggest that prophylactic administration of EPS is effective for preventing the exacerbation of PTX-induced allodynia. Aucubin may contribute to the inhibitory action of EPS on the exacerbation of PTX-induced allodynia.

  16. Pattern of semen analysis at andrology lab of Bangabandhu Sheikh Mujib Medical University: Findings and the shortcomings to overcome

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    Shakeela lshrat

    2016-08-01

    Full Text Available Background: The Infertility wing dept. of gyne & obst at BSMMU provides services to the infertile couples who are referred from primary care levels and who cannot afford the expensive private facilities. The semen analysis is performed for the male partners of infertile couples at the Andrology Lab of BSMMU for detecting male factor abnormalities. Objectives: The objective of the study is to find out the pattern of semen parameters in our population and to find out the frequency and type of abnormal semen parameters. Methods: This is a retrospective descriptive study of the semen analysis performed at the Andrology Lab of BSMMU during the year 2011. A total of 200 consecutive samples were analysed. The procedure and reference values were according to the WHO guidelines 1999. Results: Semen parameters were abnormal in 38.5% of semen analysis. Severe male factor abnormality (azospermia and severe oligospermia combined was in 28%. Sperm concentration had the highest variability followed by motility and morphology respectively in the n01moozospermic males. Conclusion: Severe oligospennia and azospermia are the most common abnormali­ties among the infertile men presenting at the Infertility unit ofBSMMU. It is recommended that the service at the Infertility wing of BSMMU should be more focused on these male factor abnormalities.

  17. Investigation of the effect of body mass index (BMI on semen parameters and male reproductive system hormones

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    Mehmet Zeynel Keskin

    2017-10-01

    Full Text Available Aim: To evaluate the effects of body mass index (BMI ratio on semen parameters and serum reproductive hormones. Materials and methods: The data of 454 patients who prsented to male infertility clinics in our hospital between 2014 and 2015 were analyzed retrospectively. Weight, height, serum hormone levels and semen analysis results of the patients were obtained. BMI values were calculated by using the weight and height values of the patients and they were classified as group 1 for BMI values ≤ 25 kg/m2, as group 2 for BMI values 25-30 kg/m2 and as group 3 for BMI values ≥ 30 kg/m2. Results: The mean values of BMI, semen volume, concentration, total motility, progressive motility, total progressive motile sperm count (TPMSC, normal morphology according to Kruger, head abnormality, neck abnormality, tail abnormality, FSH, LH, prolactin, T/E2, total testosterone and estradiol parameters of the patients were considered. Patients were divided according to BMI values in Group 1 (n = 165, Group 2 (n = 222 and Group 3 (n = 56. There was no statistically significant difference in terms of all variables between the groups. Conclusions: We analyzed the relationship between BMI level and semen parameters and reproductive hormones, demonstrating no relationship between BMI and semen parameters. In our study, BMI does not affect semen parameters although it shows negative correlation with prolactin and testosterone levels.

  18. Investigation of the effect of body mass index (BMI) on semen parameters and male reproductive system hormones.

    Science.gov (United States)

    Keskin, Mehmet Zeynel; Budak, Salih; Aksoy, Evrim Emre; Yücel, Cem; Karamazak, Serkan; Ilbey, Yusuf Ozlem; Kozacıoğlu, Zafer

    2017-10-03

    To evaluate the effects of body mass index (BMI) ratio on semen parameters and serum reproductive hormones. The data of 454 patients who prsented to male infertility clinics in our hospital between 2014 and 2015 were analyzed retrospectively. Weight, height, serum hormone levels and semen analysis results of the patients were obtained. BMI values were calculated by using the weight and height values of the patients and they were classified as group 1 for BMI values ≤ 25 kg/m2, as group 2 for BMI values 25-30 kg/m2 and as group 3 for BMI values ≥ 30 kg/m2. The mean values of BMI, semen volume, concentration, total motility, progressive motility, total progressive motile sperm count (TPMSC), normal morphology according to Kruger, head abnormality, neck abnormality, tail abnormality, FSH, LH, prolactin, T/E2, total testosterone and estradiol parameters of the patients were considered. Patients were divided according to BMI values in Group 1 (n = 165), Group 2 (n = 222) and Group 3 (n = 56). There was no statistically significant difference in terms of all variables between the groups. We analyzed the relationship between BMI level and semen parameters and reproductive hormones, demonstrating no relationship between BMI and semen parameters. In our study, BMI does not affect semen parameters although it shows negative correlation with prolactin and testosterone levels.

  19. Pregnancy and conception rate after two intravaginal inseminations with dog semen frozen either with 5% glycerol or 5% ethylene glycol.

    Science.gov (United States)

    Rota, Ada; Milani, C; Romagnoli, S; Zucchini, P; Mollo, A

    2010-03-01

    The primary goal of this study was to compare the effects of 5% ethylene glycol (EG) and 5% glycerol (G) on fertility of frozen-thawed dog semen following intravaginal insemination. The sperm-rich fraction of the ejaculate of three male dogs was collected, pooled and divided into two aliquots, and then frozen with a Tris-glucose-egg yolk-citric acid extender containing either 5% G or 5% EG. A total of 10 bitches were inseminated twice, five with G-frozen-thawed semen and five with EG-frozen-thawed semen; intravaginal inseminations were performed the 4th and the 5th day after the estimated LH peak; four straws, thawed in a 37 degrees C water bath for 1 min and diluted in a Tris buffer, were used for insemination (200 x 10(6) spermatozoa); the insemination dose was introduced in the cranial vagina of the bitch using a sterile plastic catheter. Ovariohysterectomy was performed in all bitches between days 29 and 31 after the calculated LH surge, and pregnancy status, and the number of conceptuses and corpora lutea were recorded. All bitches were pregnant. Neither the number of conceptuses, nor the ratio of conceptuses to corpora lutea (conception rate) was significantly different between groups. In this first screening, with a limited number of bitches, EG-frozen semen did not show a higher fertility than G-frozen semen when used for two intravaginal inseminations. Irrespective of the semen used, conception rate was 0.50.

  20. THE IMPROVEMENT AND APPLICATION OF EI-GUN (ELECTRICAL INSEMINATION GUN FOR ARTIFICIAL INSEMINATION (AI USING GOAT FRESH SEMEN

    Directory of Open Access Journals (Sweden)

    Mirsa Ita Dewi

    2017-03-01

    Full Text Available Electrical Insemination Gun (EI-GUN was a tool for Artificial Insemination (AI using fresh semen with an electric control system. The research objective was to produced EI-GUN for AI using fresh semen on small ruminants (goat. The research was conducted from March to June 2015. The process of EI-GUN creation implemented in Design and Prototype Laboratory and Aero Modeling Research Laboratory at Engineering Faculty, University of Brawijaya (UB. Testing tools EI-GUN on goat was conducted in the Sumber Sekar Laboratory, Animal Husbandry Faculty, UB.  The methods of research were literature study, tool specification determination, designing EI-GUN, running test, semen evaluation passed EI-GUN and application on Goat. EI-GUN has seven important parts, which supports each other in this operating system. Those parts consist of stylet, servo, system controlled, insemination pump, connecting rod, battery, and holder. EI-GUN work system sucked the semen into insemination pump as much as 5 ml, and release the semen 0.25 ml appropriate standard of AI. It was applicable for 20 times AI. The results showed that the cell motility of spermatozoa ≥ 70% were still in ranging of Indonesian National Standard (SNI.   Keywords: artificial insemination, EI-GUN, fresh semen.