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Sample records for mb bch bao

  1. A Simple Ocean Bottom Hydrophone with 200 Megabyte Data Capacity

    Science.gov (United States)

    1993-06-01

    single board computer (modified) Recorder 200 megabyte Winchester disk (by Conner Peripherals, part of the Onset Tattletale) Data format each one megabyte...system consists of this improved front-end with the addition of a commercially available single board computer -. a Tattletale model 6 from Onset...bit digitizer and recorder are part of the single board computer . The timing is performed by the computer and an external oscillator. 5 Figure 4: The

  2. High speed, very large (8 megabyte) first in/first out buffer memory (FIFO)

    Science.gov (United States)

    Baumbaugh, Alan E.; Knickerbocker, Kelly L.

    1989-01-01

    A fast FIFO (First In First Out) memory buffer capable of storing data at rates of 100 megabytes per second. The invention includes a data packer which concatenates small bit data words into large bit data words, a memory array having individual data storage addresses adapted to store the large bit data words, a data unpacker into which large bit data words from the array can be read and reconstructed into small bit data words, and a controller to control and keep track of the individual data storage addresses in the memory array into which data from the packer is being written and data to the unpacker is being read.

  3. BAO Cosmography

    Energy Technology Data Exchange (ETDEWEB)

    Lazkoz, Ruth; Escamilla-Rivera, Celia; Salzano, Vincenzo; Sendra, Irene [Fisika Teorikoaren eta Zientziaren Historia Saila, Zientzia eta Teknologia Fakultatea, Euskal Herriko Unibertsitatea, 644 Posta Kutxatila, Bilbao, 48080 (Spain); Alcaniz, Jailson, E-mail: ruth.lazkoz@ehu.es, E-mail: alcaniz@on.br, E-mail: celia_escamilla@ehu.es, E-mail: vincenzo.salzano@ehu.es, E-mail: irene.sendra@ehu.es [Observatório Nacional, Rio de Janeiro - RJ, 20921–400 (Brazil)

    2013-12-01

    Cosmography provides a model-independent way to map the expansion history of the Universe. In this paper we simulate a Euclid-like survey and explore cosmographic constraints from future Baryonic Acoustic Oscillations (BAO) observations. We derive general expressions for the BAO transverse and radial modes and discuss the optimal order of the cosmographic expansion that provides reliable cosmological constraints. Through constraints on the deceleration and jerk parameters, we show that future BAO data have the potential to provide a model-independent check of the cosmic acceleration as well as a discrimination between the standard ΛCDM model and alternative mechanisms of cosmic acceleration.

  4. ON CLASSICAL BCH CODES AND QUANTUM BCH CODES

    Institute of Scientific and Technical Information of China (English)

    Xu Yajie; Ma Zhi; Zhang Chunyuan

    2009-01-01

    It is a regular way of constructing quantum error-correcting codes via codes with self-orthogonal property, and whether a classical Bose-Chaudhuri-Hocquenghem (BCH) code is self-orthogonal can be determined by its designed distance. In this paper, we give the sufficient and necessary condition for arbitrary classical BCH codes with self-orthogonal property through algorithms. We also give a better upper bound of the designed distance of a classical narrow-sense BCH code which contains its Euclidean dual. Besides these, we also give one algorithm to compute the dimension of these codes. The complexity of all algorithms is analyzed. Then the results can be applied to construct a series of quantum BCH codes via the famous CSS constructions.

  5. The Megabyte Will Always Get Through

    Science.gov (United States)

    2012-05-17

    people by destroying the nation’s “vital centers.” Douhet identified these vital centers as industry, transportation , communications, government...University Press, 1975. Janczewski, Lech and Andrew M. Colarik. Cyber Warfare and Cyber Terrorism. Hershey: Information Science Reference, 2008

  6. Quantum BCH Codes Based on Spectral Techniques

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    When the time variable in quantum signal processing is discrete, the Fourier transform exists on the vector space of n-tuples over the Galois field F2, which plays an important role in the investigation of quantum signals. By using Fourier transforms, the idea of quantum coding theory can be described in a setting that is much different from that seen that far. Quantum BCH codes can be defined as codes whose quantum states have certain specified consecutive spectral components equal to zero and the error-correcting ability is also described by the number of the consecutive zeros. Moreover, the decoding of quantum codes can be described spectrally with more efficiency.

  7. MIB Galerkin method for elliptic interface problems

    Science.gov (United States)

    Xia, Kelin; Zhan, Meng; Wei, Guo-Wei

    2014-01-01

    Summary Material interfaces are omnipresent in the real-world structures and devices. Mathematical modeling of material interfaces often leads to elliptic partial differential equations (PDEs) with discontinuous coefficients and singular sources, which are commonly called elliptic interface problems. The development of high-order numerical schemes for elliptic interface problems has become a well defined field in applied and computational mathematics and attracted much attention in the past decades. Despite of significant advances, challenges remain in the construction of high-order schemes for nonsmooth interfaces, i.e., interfaces with geometric singularities, such as tips, cusps and sharp edges. The challenge of geometric singularities is amplified when they are associated with low solution regularities, e.g., tip-geometry effects in many fields. The present work introduces a matched interface and boundary (MIB) Galerkin method for solving two-dimensional (2D) elliptic PDEs with complex interfaces, geometric singularities and low solution regularities. The Cartesian grid based triangular elements are employed to avoid the time consuming mesh generation procedure. Consequently, the interface cuts through elements. To ensure the continuity of classic basis functions across the interface, two sets of overlapping elements, called MIB elements, are defined near the interface. As a result, differentiation can be computed near the interface as if there is no interface. Interpolation functions are constructed on MIB element spaces to smoothly extend function values across the interface. A set of lowest order interface jump conditions is enforced on the interface, which in turn, determines the interpolation functions. The performance of the proposed MIB Galerkin finite element method is validated by numerical experiments with a wide range of interface geometries, geometric singularities, low regularity solutions and grid resolutions. Extensive numerical studies confirm

  8. MIB Galerkin method for elliptic interface problems.

    Science.gov (United States)

    Xia, Kelin; Zhan, Meng; Wei, Guo-Wei

    2014-12-15

    Material interfaces are omnipresent in the real-world structures and devices. Mathematical modeling of material interfaces often leads to elliptic partial differential equations (PDEs) with discontinuous coefficients and singular sources, which are commonly called elliptic interface problems. The development of high-order numerical schemes for elliptic interface problems has become a well defined field in applied and computational mathematics and attracted much attention in the past decades. Despite of significant advances, challenges remain in the construction of high-order schemes for nonsmooth interfaces, i.e., interfaces with geometric singularities, such as tips, cusps and sharp edges. The challenge of geometric singularities is amplified when they are associated with low solution regularities, e.g., tip-geometry effects in many fields. The present work introduces a matched interface and boundary (MIB) Galerkin method for solving two-dimensional (2D) elliptic PDEs with complex interfaces, geometric singularities and low solution regularities. The Cartesian grid based triangular elements are employed to avoid the time consuming mesh generation procedure. Consequently, the interface cuts through elements. To ensure the continuity of classic basis functions across the interface, two sets of overlapping elements, called MIB elements, are defined near the interface. As a result, differentiation can be computed near the interface as if there is no interface. Interpolation functions are constructed on MIB element spaces to smoothly extend function values across the interface. A set of lowest order interface jump conditions is enforced on the interface, which in turn, determines the interpolation functions. The performance of the proposed MIB Galerkin finite element method is validated by numerical experiments with a wide range of interface geometries, geometric singularities, low regularity solutions and grid resolutions. Extensive numerical studies confirm the

  9. Mutational analysis of three bchH paralogs in (bacterio-)chlorophyll biosynthesis in Chlorobaculum tepidum

    DEFF Research Database (Denmark)

    Gomez Maqueo Chew, Aline; Frigaard, Niels-Ulrik; Bryant, Donald A

    2009-01-01

    The first committed step in the biosynthesis of (bacterio-)chlorophyll is the insertion of Mg2+ into protoporphyrin IX by Mg-chelatase. In all known (B)Chl-synthesizing organisms, Mg-chelatase is encoded by three genes that are homologous to bchH, bchD, and bchI of Rhodobacter spp. The genomes...... of all sequenced strains of green sulfur bacteria (Chlorobi) encode multiple bchH paralogs, and in the genome of Chlorobaculum tepidum, there are three bchH paralogs, denoted CT1295 (bchT), CT1955 (bchS), and CT1957 (bchH). Cba. tepidum mutants lacking one or two of these paralogs were constructed...... and characterized. All of the mutants lacking only one of these BchH homologs, as well as bchS bchT and bchH bchT double mutants, which can only produce BchH or BchS, respectively, were viable. However, attempts to construct a bchH bchS double mutant, in which only BchT was functional, were consistently...

  10. Isolating the Lyman Alpha Forest BAO Anomaly

    CERN Document Server

    Evslin, Jarah

    2016-01-01

    A 2.5-3 sigma discrepancy has been reported between the baryonic acoustic oscillation peak (BAO) in the Lyman alpha forest at z=2.34 and the best fit Planck LCDM cosmology. To isolate the origin of the tension, we consider unanchored BAO, in which the standard BAO ruler is not calibrated, eliminating any dependence on cosmology before redshift z=2.34. We consider BOSS BAO measurements at z=0.32, 0.57 and 2.34, using the full 2-dimensional constraints on the best and worst determined combinations of the angular and line of sight BAO scale, as well as isotropic BAO measurements by 6dF and SDSS at z=0.106 and z=0.15. We find that the z>0.43 data alone is in 2.8 sigma of tension with LCDM with or without the Planck best fit values of the mass fraction and the BAO scale, indicating that the tension arises not from the LCDM parameters but from the dark energy evolution itself at 0.57BAO data, which is itself consistent with LCDM, reduces the tension to just over 2 sigma, however i...

  11. Biodegradation of MIB and geosmin with slow sand filters.

    Science.gov (United States)

    Hsieh, Shu-Ting; Lin, Tsair-Fuh; Wang, Gen-Shuh

    2010-01-01

    This study evaluated the biodegradation of MIB (2-methylisoborneol) and geosmin (trans-1,10-dimethyl-trans- 9-decalol) in simulated slow sand filtration (SSF) columns and in batch reactors. The results showed that both MIB and geosmin were biodegradable in the two systems. In batch experiments, the overall removals for MIB and geosmin were 50% and 78%, respectively, after 7 days of contact time. Volatilization loss plays an important role for geosmin in batch systems. Simulated SSF column studies also showed that more than 50% of geosmin and MIB were degraded by the microbial on the sand surface of a slow sand filter. With a filtration rate of 5 m/day, the simulated SSF degraded MIB from 48% to 69% and geosmin from 87% to 96%. The rapid biodegradation of MIB and geosmin in SSF column tests was attributed to the use of filter sands from the SSF unit in the Kinmen water treatment plant, where the microbial had been acclimated to both MIB and geosmin. The results also showed that more than 70% of the geosmin was removed in the top portion of the filter ( approximately 10 cm); while the removal of MIB occurred throughout the entire column depth. The results of this study demonstrated that slow flow through preacclimated sand was effective for control of MIB and geosmin in drinking water.

  12. MIB-1 expression and iododeoxyuridine labelling in soft tissue sarcomas

    DEFF Research Database (Denmark)

    Jensen, V; Høyer, M; Sørensen, Flemming Brandt

    1996-01-01

    the variables and with mitotic score were investigated. Proliferative activity was assessed by use of monoclonal antibody MIB-1 and staining for iododeoxyuridine (IdUrd), and evaluated in multiple, random, systematically sampled fields of vision. MIB-1 indices were higher than those of IdUrd but for each case...... the two values were positively correlated (r = 0.78). The MIB-1 index correlated positively with mitotic score (2P MIB-1 and IdUrd indices were excellent (r = 0.98 and r = 0.90, respectively). p53 expression was detected...... in 43% and strong bcl-2 expression was present in 57% of the studied cases. Expression of p53 and bcl-2 were not significantly correlated to proliferative activity or the histological features. We conclude, that the MIB-1 index is a reliable and reproducible estimate of proliferative activity and might...

  13. Troxacitabine: BCH 4556, SPD 758, Troxatyl.

    Science.gov (United States)

    2003-01-01

    Troxacitabine [BCH 4556; SPD 758; Troxatyl] is a DNA synthesis inhibitor. This profile has been selected from R&D Insight, a pharmaceutical intelligence database produced by Adis International Ltd. It is a member of a novel class of nucleoside analogues discovered by BioChem Pharma and is the first example of a synthetic L-nucleoside analogue to have shown anticancer activity in animal models. On 11 May 2001, BioChem Pharma was acquired by, and integrated into, Shire Pharmaceuticals Group. In February 2002 Shire announced that it intended to pursue development of troxacitabine as a treatment for solid tumours. In addition, Shire indicated that it would pursue the drug's development for acute myeloid leukaemia. In March 1999, phase II trials were initiated to investigate the efficacy and tolerability of troxacitabine in a variety of solid tumours including pancreas, prostate, colorectal, renal and non-small cell lung cancers and melanoma. The trials were conducted throughout North America and were closed to patient accrual in 2000. Two phase I combination chemotherapy trials in solid tumours (one with cisplatin and another with paclitaxel) have been initiated. One of these trials is in patients with pancreatic cancer. A phase III trial in patients with pancreatic cancer is expected to begin during the second or third quarter of 2003. In addition, further clinical development was initiated in May 2000, in the form of a combination chemotherapy trial in patients with acute leukaemia. A phase II trial in patients with acute myeloid leukaemia (AML) and chronic myeloid leukaemia-blast phase (CML-BP) has reported that troxacitabine demonstrated significant activity in these cancers. However, Shire indicated that no further development for CML-BP will be conducted. The company indicated that it would focus future development in the haematological malignancy area on AML and has initiated an exploratory phase III trial of troxacitabine in previously untreated patients with

  14. Modeling the reconstructed BAO in Fourier space

    Science.gov (United States)

    Seo, Hee-Jong; Beutler, Florian; Ross, Ashley J.; Saito, Shun

    2016-08-01

    The density field reconstruction technique, which partially reverses the non-linear degradation of the Baryon acoustic oscillation (BAO) feature in the galaxy redshift surveys, has been successful in substantially improving the cosmology constraints from recent surveys such as Baryon Oscillation Spectroscopic Survey (BOSS). We estimate the efficiency of the method as a function of various reconstruction details. To directly quantify the BAO information in non-linear density fields before and after reconstruction, we calculate the cross-correlations (i.e. propagators) of the pre(post)-reconstructed density field with the initial linear field using a mock sample that mimics the clustering of the BOSS galaxies. The results directly provide the BAO damping as a function of wavenumber that can be implemented into the Fisher matrix analysis. We focus on investigating the dependence of the propagator on a choice of smoothing filters and on two major different conventions of the redshift-space density field reconstruction that have been used in literature. By estimating the BAO signal to noise for each case, we predict constraints on the angular diameter distance and Hubble parameter using the Fisher matrix analysis. We thus determine an optimal Gaussian smoothing filter scale for the signal-to-noise level of the BOSS CMASS. We also present appropriate BAO fitting models for different reconstruction methods based on the first- and second-order Lagrangian perturbation theory in Fourier space. Using the mock data, we show that the modified BAO fitting model can substantially improve the accuracy of the BAO position in the best fits as well as the goodness of the fits.

  15. Rate-adaptive BCH codes for distributed source coding

    DEFF Research Database (Denmark)

    Salmistraro, Matteo; Larsen, Knud J.; Forchhammer, Søren

    2013-01-01

    This paper considers Bose-Chaudhuri-Hocquenghem (BCH) codes for distributed source coding. A feedback channel is employed to adapt the rate of the code during the decoding process. The focus is on codes with short block lengths for independently coding a binary source X and decoding it given its...... strategies for improving the reliability of the decoded result are analyzed, and methods for estimating the performance are proposed. In the analysis, noiseless feedback and noiseless communication are assumed. Simulation results show that rate-adaptive BCH codes achieve better performance than low...... correlated side information Y. The proposed codes have been analyzed in a high-correlation scenario, where the marginal probability of each symbol, Xi in X, given Y is highly skewed (unbalanced). Rate-adaptive BCH codes are presented and applied to distributed source coding. Adaptive and fixed checking...

  16. New quantum codes constructed from quaternary BCH codes

    Science.gov (United States)

    Xu, Gen; Li, Ruihu; Guo, Luobin; Ma, Yuena

    2016-10-01

    In this paper, we firstly study construction of new quantum error-correcting codes (QECCs) from three classes of quaternary imprimitive BCH codes. As a result, the improved maximal designed distance of these narrow-sense imprimitive Hermitian dual-containing quaternary BCH codes are determined to be much larger than the result given according to Aly et al. (IEEE Trans Inf Theory 53:1183-1188, 2007) for each different code length. Thus, families of new QECCs are newly obtained, and the constructed QECCs have larger distance than those in the previous literature. Secondly, we apply a combinatorial construction to the imprimitive BCH codes with their corresponding primitive counterpart and construct many new linear quantum codes with good parameters, some of which have parameters exceeding the finite Gilbert-Varshamov bound for linear quantum codes.

  17. Boffins send 60 million MB around the world in 10 days

    CERN Multimedia

    Farrell, Nick

    2005-01-01

    In one of the world's biggest grid computing projects, UK boffins at Rutherford Appleton Laboratory (EAL) in Oxfordshire managed to transfer 60 million megabytes of data aroung the world in ten days (1/2 page)

  18. Modeling the reconstructed BAO in Fourier space

    CERN Document Server

    Seo, Hee-Jong; Ross, Ashley J; Saito, Shun

    2015-01-01

    The density field reconstruction technique, which was developed to partially reverse the nonlinear degradation of the Baryon Acoustic Oscillation (BAO) feature in the galaxy redshift surveys, has been successful in substantially improving the cosmology constraints from recent galaxy surveys such as Baryon Oscillation Spectroscopic Survey (BOSS). We estimate the efficiency of the reconstruction method as a function of various reconstruction details. To directly quantify the BAO information in nonlinear density fields before and after reconstruction, we calculate the cross-correlations (i.e., propagators) of the pre(post)-reconstructed density field with the initial linear field using a mock galaxy sample that is designed to mimic the clustering of the BOSS CMASS galaxies. The results directly provide the BAO damping as a function of wavenumber that can be implemented into the Fisher matrix analysis. We focus on investigating the dependence of the propagator on a choice of smoothing filters and on two major dif...

  19. Performance of FSO-OFDM based on BCH code

    Directory of Open Access Journals (Sweden)

    Jiao Xiao-lu

    2016-01-01

    Full Text Available As contrasted with the traditional OOK (on-off key system, FSO-OFDM system can resist the atmospheric scattering and improve the spectrum utilization rate effectively. Due to the instability of the atmospheric channel, the system will be affected by various factors, and resulting in a high BER. BCH code has a good error correcting ability, particularly in the short-length and medium-length code, and its performance is close to the theoretical value. It not only can check the burst errors but also can correct the random errors. Therefore, the BCH code is applied to the system to reduce the system BER. At last, the semi-physical simulation has been conducted with MATLAB. The simulation results show that when the BER is 10-2, the performance of OFDM is superior 4dB compared with OOK. In different weather conditions (extension rain, advection fog, dust days, when the BER is 10-5, the performance of BCH (255,191 channel coding is superior 4~5dB compared with uncoded system. All in all, OFDM technology and BCH code can reduce the system BER.

  20. A class of constacyclic BCH codes and new quantum codes

    Science.gov (United States)

    liu, Yang; Li, Ruihu; Lv, Liangdong; Ma, Yuena

    2017-03-01

    Constacyclic BCH codes have been widely studied in the literature and have been used to construct quantum codes in latest years. However, for the class of quantum codes of length n=q^{2m}+1 over F_{q^2} with q an odd prime power, there are only the ones of distance δ ≤ 2q^2 are obtained in the literature. In this paper, by a detailed analysis of properties of q2-ary cyclotomic cosets, maximum designed distance δ _{max} of a class of Hermitian dual-containing constacyclic BCH codes with length n=q^{2m}+1 are determined, this class of constacyclic codes has some characteristic analog to that of primitive BCH codes over F_{q^2}. Then we can obtain a sequence of dual-containing constacyclic codes of designed distances 2q^2 2q^2 can be constructed from these dual-containing codes via Hermitian Construction. These newly obtained quantum codes have better code rate compared with those constructed from primitive BCH codes.

  1. The Work of Ngo Bao Chau

    CERN Document Server

    Hales, Thomas C

    2010-01-01

    In August 2010, Ngo Bao Chau was awarded a Fields Medal for his deep work relating the Hitchin fibration to the Arthur-Selberg trace formula, and in particular for his proof of the Fundamental Lemma for Lie algebras. This article gives a brief introduction to his work for a general mathematical audience.

  2. Comparative study on the removal technologies of 2-methylisoborneol (MIB) in drinking water

    Institute of Scientific and Technical Information of China (English)

    LIANG Cun-zhen; WANG Dong-sheng; GE Xiao-peng; YANG Min; SUN Wei

    2006-01-01

    Removal of 2-methylisoborneol (MIB) in drinking water by ozone, powdered activated carbon (PAC), potassium permanganate and potassium ferrate was investigated. The adsorption kinetics of MIB by both wood-based and coat-based PACs show that main removal of MIB occurs within contact time of 1 h. Compared with the wood-based PAC, the coat-based PAC evidently improved the removal efficiency of MIB. The removal percentage of trace MIB at any given time for a particular carbon dosage was irrelative to the initial concentration of MIB. A series of experiments were performed to determine the effect of pH on the ozonation of MIB. The results show that pH has a significant effect on the ozonation of MIB. It is conclusive that potassium permanganate and potassium ferrate are ineffective in removing the MIB in drinking water.

  3. Antígeno MIB-1 em nefroblastomas MIB-1 antigen in nephroblastomas

    Directory of Open Access Journals (Sweden)

    Antonio Carlos Pereira Martins

    2000-01-01

    Full Text Available Foram estudados 35 pacientes tratados com nefrectomia radical e quimioterapia, em 17 (48,5% dos quais se associou também a radioterapia. A idade média dos pacientes foi de 38± 29 meses e o tempo de seguimento pós-operatório de 69± 66 meses. Óbito pela neoplasia ocorreu em 5 pacientes, todos com histologia favorável. As peças conservadas em formol ou blocos de parafina para imunhistoquímica com anticorpo MIB-1, método da avidina-biotina-peroxidase. Em 13/35 (37,1% dos tumores a marcação para o MIB-1 foi positiva. As proporções respectivas de marcação para blastema, epitélio e estroma foram: 34,6%, 18,7% e 0%. Não houve diferença estatística entre a marcação imunohistoquímica e com o tipo histológico, estádio e sobrevida dos pacientes.

  4. Geometrical Constraint on Curvature with BAO experiments

    CERN Document Server

    Takada, Masahiro

    2015-01-01

    The spatial curvature ($K$ or $\\Omega_K$) is one of the most fundamental parameters of isotropic and homogeneous universe and has a close link to the physics of early universe. Combining the radial and angular diameter distances measured via the baryon acoustic oscillation (BAO) experiments allows us to unambiguously constrain the curvature. The method is primarily based on the metric theory, but not much on the theory of structure formation other than the existence of BAO scale and is free of any model of dark energy. In this paper, we estimate a best-achievable accuracy of constraining the curvature with the BAO experiments. We show that an all-sky, cosmic-variance-limited galaxy survey covering the universe up to $z>4$ enables a precise determination of the curvature to an accuracy of $\\sigma(\\Omega_K)\\simeq 10^{-3}$. When we assume a model of dark energy, either the cosmological constraint or the $(w_0,w_a)$-model, it can achieve a precision of $\\sigma(\\Omega_K)\\simeq \\mbox{a few}\\times 10^{-4}$. These fo...

  5. The Influence of Yu’e Bao on Commercial Banks

    Institute of Scientific and Technical Information of China (English)

    陈云阳

    2014-01-01

    Yu’e Bao is the financial innovation of modern market environment, and Yu’e Bao has been widely used as a financial tool. But Yu’e Bao might impact commercial banks negatively. People’s atitudes toward Yu’e Bao are important for policy makers. The paper pays atention to the reasons why Yu’e Bao is so successful and the innovation of Yu’ e Bao. Then the paper atempts to find that what Yu’e Bao has specific influence on commercial banks. Through the analysis of the specific impact of Yu’e Bao on the commercial banks, the paper shows that commercial banks can do some appropriate changes in order to get a beter development. What is more, the paper also suggests that Yu’e Bao wil not get in the way of banks’ development in the long run. On the contrary, Yu’e Bao explores a road for the commercial banks’ future development.

  6. Evaluating MIB-II Implementations : A Comparison of Management Functionality Within Routers

    NARCIS (Netherlands)

    Holland, Henrik E.

    2002-01-01

    This study deals with the methodology, realisation and execution of MIB-2 evaluation tests. The evaluation was limited to approximately 40 separate MIB-2 objects from 5 MIB-2 groups. The correctness and availability of these objects was determined under a number of test conditions. One 10 Mbit and t

  7. A New Simple Ultrahigh Speed Decoding Algorithm for BCH Code

    Institute of Scientific and Technical Information of China (English)

    唐建军; 纪越峰

    2002-01-01

    In order to content with forward error correction (FEC) technology of the high-speed optical communication system, a new simple decoding algorithm for triple-error correcting Bose, Chaudhuri, and Hocquenghem (BCH) code is proposed. Without complicated matrix-operation or division-operation or intricate iterative algorithm, the algorithm is high efficient and high-speed because of its simplicity in structure. The result of hardware emulation confirms that the algorithm is feasible completely. Introduction of the parallel structure increases the speed of coding greatly. The algorithm can be used in the high-speed optical communication system and other fields.

  8. Bao Huiqiao—Pianist Optimistically Confronts Life

    Institute of Scientific and Technical Information of China (English)

    1996-01-01

    MEMBERS of the audience listening to the melodious melody coming from the keyboards were unable to believe that the pianist had studied piano for only two months. The event was a special piano recital held on one June Sunday afternoon in the auditorium at the Dongcheng Normal School in Beijing. The pianists, the oldest 61 and the youngest 22, were al adult students at the Bao Huiqiao Piano Center, the first center in the country named after the famed Chinese pianist who serves as chairperson of the board. One performer, 61-year-old He Jiyuan,

  9. Revisiting the BaO2/BaO redox cycle for solar thermochemical energy storage.

    Science.gov (United States)

    Carrillo, A J; Sastre, D; Serrano, D P; Pizarro, P; Coronado, J M

    2016-03-21

    The barium peroxide-based redox cycle was proposed in the late 1970s as a thermochemical energy storage system. Since then, very little attention has been paid to such redox couples. In this paper, we have revisited the use of reduction-oxidation reactions of the BaO2/BaO system for thermochemical heat storage at high temperatures. Using thermogravimetric analysis, reduction and oxidation reactions were studied in order to find the main limitations associated with each process. Furthermore, the system was evaluated through several charge-discharge stages in order to analyse its possible degradation after repeated cycling. Through differential scanning calorimetry the heat stored and released were also determined. Oxidation reaction, which was found to be slower than reduction, was studied in more detail using isothermal tests. It was observed that the rate-controlling step of BaO oxidation follows zero-order kinetics, although at high temperatures a deviation from Arrhenius behaviour was observed probably due to hindrances to anionic oxygen diffusion caused by the formation of an external layer of BaO2. This redox couple was able to withstand several redox cycles without deactivation, showing reaction conversions close to 100% provided that impurities are previously eliminated through thermal pre-treatment, demonstrating the feasibility of this system for solar thermochemical heat storage.

  10. Multirate Filter Bank Representations of RS and BCH Codes

    Directory of Open Access Journals (Sweden)

    Marc Moonen

    2009-01-01

    Full Text Available This paper addresses the use of multirate filter banks in the context of error-correction coding. An in-depth study of these filter banks is presented, motivated by earlier results and applications based on the filter bank representation of Reed-Solomon (RS codes, such as Soft-In Soft-Out RS-decoding or RS-OFDM. The specific structure of the filter banks (critical subsampling is an important aspect in these applications. The goal of the paper is twofold. First, the filter bank representation of RS codes is now explained based on polynomial descriptions. This approach allows us to gain new insight in the correspondence between RS codes and filter banks. More specifically, it allows us to show that the inherent periodically time-varying character of a critically subsampled filter bank matches remarkably well with the cyclic properties of RS codes. Secondly, an extension of these techniques toward the more general class of BCH codes is presented. It is demonstrated that a BCH code can be decomposed into a sum of critically subsampled filter banks.

  11. Multirate Filter Bank Representations of RS and BCH Codes

    Directory of Open Access Journals (Sweden)

    Van Meerbergen Geert

    2008-01-01

    Full Text Available Abstract This paper addresses the use of multirate filter banks in the context of error-correction coding. An in-depth study of these filter banks is presented, motivated by earlier results and applications based on the filter bank representation of Reed-Solomon (RS codes, such as Soft-In Soft-Out RS-decoding or RS-OFDM. The specific structure of the filter banks (critical subsampling is an important aspect in these applications. The goal of the paper is twofold. First, the filter bank representation of RS codes is now explained based on polynomial descriptions. This approach allows us to gain new insight in the correspondence between RS codes and filter banks. More specifically, it allows us to show that the inherent periodically time-varying character of a critically subsampled filter bank matches remarkably well with the cyclic properties of RS codes. Secondly, an extension of these techniques toward the more general class of BCH codes is presented. It is demonstrated that a BCH code can be decomposed into a sum of critically subsampled filter banks.

  12. Detecting the BAO using Discrete Wavelet Packets

    Science.gov (United States)

    Garcia, Noel Anthony; Wu, Yunyun; Kadowaki, Kevin; Pando, Jesus

    2017-01-01

    We use wavelet packets to investigate the clustering of matter on galactic scales in search of the Baryon Acoustic Oscillations. We do so in two ways. We develop a wavelet packet approach to measure the power spectrum and apply this method to the CMASS galaxy catalogue from the Sloan Digital Sky Survey (SDSS). We compare the resulting power spectrum to published BOSS results by measuring a parameter β that compares our wavelet detected oscillations to the results from the SDSS collaboration. We find that β=1 indicating that our wavelet packet methods are detecting the BAO at a similar level as traditional Fourier techniques. We then use wavelet packets to decompose, denoise, and then reconstruct the galaxy density field. Using this denoised field, we compute the standard two-point correlation function. We are able to successfully detect the BAO at r ≈ 105 h-1 Mpc in line with previous SDSS results. We conclude that wavelet packets do reproduce the results of the key clustering statistics computed by other means. The wavelet packets show distinct advantages in suppressing high frequency noise and in keeping information localized.

  13. Influence of BCH and LDPC Code Parameters on the BER Characteristic of Satellite DVB Channels

    Directory of Open Access Journals (Sweden)

    L. Jordanova

    2014-02-01

    Full Text Available This article presents the results of a study on the noise immunity of DVB channels when higher-order M-ary APSK modulation schemes and concatenated BCH-LDPC codes are used. Dependencies to determine the probability at the decoder output are given taking into consideration the BCH and LDPC code parameters and the error probability in the communication channel. The influence of the BCH packets length, the BCH code rate, the number of maximum iteration and the parameters of LDPC parity-check matrix on the code efficiency is analyzed. Research of the influence of the concatenated LDPC-BCH code parameters on the radio channel noise immunity is conducted and dependencies to determine the required CNR at the input of the satellite receiver are given.

  14. Cocrystallization studies of full-length recombinant butyrylcholinesterase (BChE) with cocaine

    Energy Technology Data Exchange (ETDEWEB)

    Asojo, Oluwatoyin Ajibola; Asojo, Oluyomi Adebola; Ngamelue, Michelle N.; Homma, Kohei; Lockridge, Oksana (Nebraska-Med)

    2011-09-16

    Human butyrylcholinesterase (BChE; EC 3.1.1.8) is a 340 kDa tetrameric glycoprotein that is present in human serum at about 5 mg l{sup -1} and has well documented therapeutic effects on cocaine toxicity. BChE holds promise as a therapeutic that reduces and finally eliminates the rewarding effects of cocaine, thus weaning an addict from the drug. There have been extensive computational studies of cocaine hydrolysis by BChE. Since there are no reported structures of BChE with cocaine or any of the hydrolysis products, full-length monomeric recombinant wild-type BChE was cocrystallized with cocaine. The refined 3 {angstrom} resolution structure appears to retain the hydrolysis product benzoic acid in sufficient proximity to form a hydrogen bond to the active-site Ser198.

  15. Fault Tolerant Parallel Filters Based On Bch Codes

    Directory of Open Access Journals (Sweden)

    K.Mohana Krishna

    2015-04-01

    Full Text Available Digital filters are used in signal processing and communication systems. In some cases, the reliability of those systems is critical, and fault tolerant filter implementations are needed. Over the years, many techniques that exploit the filters’ structure and properties to achieve fault tolerance have been proposed. As technology scales, it enables more complex systems that incorporate many filters. In those complex systems, it is common that some of the filters operate in parallel, for example, by applying the same filter to different input signals. Recently, a simple technique that exploits the presence of parallel filters to achieve multiple fault tolerance has been presented. In this brief, that idea is generalized to show that parallel filters can be protected using Bose– Chaudhuri–Hocquenghem codes (BCH in which each filter is the equivalent of a bit in a traditional ECC. This new scheme allows more efficient protection when the number of parallel filters is large.

  16. Eulerian BAO Reconstructions and N-Point Statistics

    CERN Document Server

    Schmittfull, Marcel; Beutler, Florian; Sherwin, Blake; Chu, Man Yat

    2015-01-01

    As galaxy surveys begin to measure the imprint of baryonic acoustic oscillations (BAO) on large-scale structure at the sub-percent level, reconstruction techniques that reduce the contamination from nonlinear clustering become increasingly important. Inverting the nonlinear continuity equation, we propose an Eulerian growth-shift reconstruction algorithm that does not require the displacement of any objects, which is needed for the standard Lagrangian BAO reconstruction algorithm. In our simulations, the algorithm yields 95% of the BAO signal-to-noise obtained from standard reconstruction. The reconstructed power spectrum is obtained by adding specific simple 3- and 4-point statistics to the pre-reconstruction power spectrum, making it very transparent how additional BAO information from higher-point statistics is included in the power spectrum through the reconstruction process. Analytical models of the reconstructed density for the two algorithms agree at second order. Based on similar modeling efforts, we ...

  17. Evolving BioAssay Ontology (BAO): modularization, integration and applications.

    Science.gov (United States)

    Abeyruwan, Saminda; Vempati, Uma D; Küçük-McGinty, Hande; Visser, Ubbo; Koleti, Amar; Mir, Ahsan; Sakurai, Kunie; Chung, Caty; Bittker, Joshua A; Clemons, Paul A; Brudz, Steve; Siripala, Anosha; Morales, Arturo J; Romacker, Martin; Twomey, David; Bureeva, Svetlana; Lemmon, Vance; Schürer, Stephan C

    2014-01-01

    The lack of established standards to describe and annotate biological assays and screening outcomes in the domain of drug and chemical probe discovery is a severe limitation to utilize public and proprietary drug screening data to their maximum potential. We have created the BioAssay Ontology (BAO) project (http://bioassayontology.org) to develop common reference metadata terms and definitions required for describing relevant information of low-and high-throughput drug and probe screening assays and results. The main objectives of BAO are to enable effective integration, aggregation, retrieval, and analyses of drug screening data. Since we first released BAO on the BioPortal in 2010 we have considerably expanded and enhanced BAO and we have applied the ontology in several internal and external collaborative projects, for example the BioAssay Research Database (BARD). We describe the evolution of BAO with a design that enables modeling complex assays including profile and panel assays such as those in the Library of Integrated Network-based Cellular Signatures (LINCS). One of the critical questions in evolving BAO is the following: how can we provide a way to efficiently reuse and share among various research projects specific parts of our ontologies without violating the integrity of the ontology and without creating redundancies. This paper provides a comprehensive answer to this question with a description of a methodology for ontology modularization using a layered architecture. Our modularization approach defines several distinct BAO components and separates internal from external modules and domain-level from structural components. This approach facilitates the generation/extraction of derived ontologies (or perspectives) that can suit particular use cases or software applications. We describe the evolution of BAO related to its formal structures, engineering approaches, and content to enable modeling of complex assays and integration with other ontologies and

  18. Fuzzy dot ideals and fuzzy dot H-ideals of BCH-algebras

    Institute of Scientific and Technical Information of China (English)

    PENG Jia-yin

    2008-01-01

    The notions of fuzzy dot ideals and fuzzy dot H-ideals in BCH-algebras are intro duced,several appropriate examples are provided,and their some properties are investigated.The relations among fuzzy ideal,fuzzy H-ideal,fuzzy dot ideal and fuzzy dot H-ideals in BCH algebras are discussed,several equivalent depictions of fuzzy dot ideal are obtained. How to deal with the homomorphic image and inverse image of fuzzy dot ideals (fuzzy dot H-ideals) are studied. The relations between a fuzzy dot ideal (fuzzy dot H-ideal) in BCH-algebras and a fuzzy dot ideal (fuzzy dot H-ideal) in the product algebra of BCH-algebras are given.

  19. Novel BCH Code Design for Mitigation of Phase Noise Induced Cycle Slips in DQPSK Systems

    DEFF Research Database (Denmark)

    Leong, M. Y.; Larsen, Knud J.; Jacobsen, G.

    2014-01-01

    We show that by proper code design, phase noise induced cycle slips causing an error floor can be mitigated for 28 Gbau d DQPSK systems. Performance of BCH codes are investigated in terms of required overhead......We show that by proper code design, phase noise induced cycle slips causing an error floor can be mitigated for 28 Gbau d DQPSK systems. Performance of BCH codes are investigated in terms of required overhead...

  20. Removal of 2-MIB and geosmin using UV/persulfate: contributions of hydroxyl and sulfate radicals.

    Science.gov (United States)

    Xie, Pengchao; Ma, Jun; Liu, Wei; Zou, Jing; Yue, Siyang; Li, Xuchun; Wiesner, Mark R; Fang, Jingyun

    2015-02-01

    2-methylisoborneol (2-MIB) and geosmin are two odor-causing compounds that are difficult to remove and the cause of many consumer complaints. In this study, we assessed the degradation of 2-MIB and geosmin using a UV/persulfate process for the first time. The results showed that both 2-MIB and geosmin could be degraded effectively using this process. The process was modeled based on steady-state assumption with respect to the odor-causing compounds and either hydroxyl or sulfate radicals. The second order rate constants for 2-MIB and geosmin reacting with the sulfate radical (SO4(-)) were estimated to be (4.2 ± 0.6) × 10(8) M(-1)s(-1) and (7.6 ± 0.6) × 10(8) M(-1)s(-1) respectively at a pH of 7.0. The contributions of the hydroxyl radical (OH) to 2-MIB and geosmin degradation were 3.5 times and 2.0 times higher, respectively, than the contribution from SO4(-) in Milli-Q water with 2 mM phosphate buffer at pH 7.0. The pseudo-first-order rate constants (ko(s)) of both 2-MIB and geosmin increased with increasing dosages of persulfate. Although pH did not affect the degradation of 2-MIB and geosmin directly, different scavenging effects of hydrogen phosphate and dihydrogen phosphate resulted in higher values of ko(s) for both 2-MIB and geosmin in acidic condition. Bicarbonate and natural organic matter (NOM) inhibited the degradation of both 2-MIB and geosmin dramatically through consuming OH and SO4(-) and were likely to be the main radical scavengers in natural waters when using UV/persulfate process to control 2-MIB and geosmin.

  1. Cosmological implications of baryon acoustic oscillation (BAO) measurements

    CERN Document Server

    Aubourg, Éric; Bautista, Julian E; Beutler, Florian; Bhardwaj, Vaishali; Bizyaev, Dmitry; Blanton, Michael; Blomqvist, Michael; Bolton, Adam S; Bovy, Jo; Brewington, Howard; Brinkmann, J; Brownstein, Joel R; Burden, Angela; Busca, Nicolás G; Carithers, William; Chuang, Chia-Hsun; Comparat, Johan; Cuesta, Antonio J; Dawson, Kyle S; Delubac, Timothée; Eisenstein, Daniel J; Font-Ribera, Andreu; Ge, Jian; Goff, J -M Le; Gontcho, Satya Gontcho A; Gott, J Richard; Gunn, James E; Guo, Hong; Guy, Julien; Hamilton, Jean-Christophe; Ho, Shirley; Honscheid, Klaus; Howlett, Cullan; Kirkby, David; Kitaura, Francisco S; Kneib, Jean-Paul; Lee, Khee-Gan; Long, Dan; Lupton, Robert H; Magaña, Mariana Vargas; Malanushenko, Viktor; Malanushenko, Elena; Manera, Marc; Maraston, Claudia; Margala, Daniel; McBride, Cameron K; Miralda-Escudé, Jordi; Myers, Adam D; Nichol, Robert C; Noterdaeme, Pasquier; Nuza, Sebastián E; Olmstead, Matthew D; Oravetz, Daniel; Pâris, Isabelle; Padmanabhan, Nikhil; Palanque-Delabrouille, Nathalie; Pan, Kaike; Pellejero-Ibanez, Marcos; Percival, Will J; Petitjean, Patrick; Pieri, Matthew M; Prada, Francisco; Reid, Beth; Roe, Natalie A; Ross, Ashley J; Ross, Nicholas P; Rossi, Graziano; Rubiño-Martín, Jose Alberto; Sánchez, Ariel G; Samushia, Lado; Santos, Ricardo Tanausú Génova; Scóccola, Claudia G; Schlegel, David J; Schneider, Donald P; Seo, Hee-Jong; Sheldon, Erin; Simmons, Audrey; Skibba, Ramin A; Slosar, Anže; Strauss, Michael A; Thomas, Daniel; Tinker, Jeremy L; Tojeiro, Rita; Vazquez, Jose Alberto; Viel, Matteo; Wake, David A; Weaver, Benjamin A; Weinberg, David H; Wood-Vasey, W M; Yèche, Christophe; Zehavi, Idit; Zhao, Gong-Bo

    2014-01-01

    We derive constraints on cosmological parameters and tests of dark energy models from the combination of baryon acoustic oscillation (BAO) measurements with cosmic microwave background (CMB) and Type Ia supernova (SN) data. We take advantage of high-precision BAO measurements from galaxy clustering and the Ly-alpha forest (LyaF) in the BOSS survey of SDSS-III. BAO data alone yield a high confidence detection of dark energy, and in combination with the CMB angular acoustic scale they further imply a nearly flat universe. Combining BAO and SN data into an "inverse distance ladder" yields a 1.7% measurement of $H_0=67.3 \\pm1.1$ km/s/Mpc. This measurement assumes standard pre-recombination physics but is insensitive to assumptions about dark energy or space curvature, so agreement with CMB-based estimates that assume a flat LCDM cosmology is an important corroboration of this minimal cosmological model. For open LCDM, our BAO+SN+CMB combination yields $\\Omega_m=0.301 \\pm 0.008$ and curvature $\\Omega_k=-0.003 \\pm ...

  2. Comparison between ozone and ferrate in oxidising geosmin and 2-MIB in water.

    Science.gov (United States)

    Park, G; Yu, M; Go, J; Kim, E; Kim, H

    2007-01-01

    Among the chemicals causing taste and odour (T&O) in drinking water, the most commonly identified and problematic ones are geosmin and 2-MIB (2-methylisoborneol). Since the reported odour thresholds of geosmin and 2-MIB are as low as 4 and 8.5 ng/L, respectively, they are not readily removed by conventional water treatment processes. In this study, ozone (O3) and ferrate (Fe(VI)) were applied to oxidise geosmin and 2-MIB. Their performances were compared in terms of removal efficiency of geosmin and 2-MIB. In the case of O3, removal efficiency of geosmin and 2-MIB ozonation at different initial O3 doses, H2O2/O3 ratios and water temperatures were evaluated. The oxidation rates of geosmin and 2-MIB by Fe(VI) were measured within pH 6-8. The effect of H2O2 addition was also evaluated. In summary, O3, especially with H2O2, could almost completely oxidise geosmin and 2-MIB, while Fe(VI) could not oxidise them more than 25% at any pH that was considered in this study. This was attributed to the structure of the organics and high reaction selectivity of Fe(VI). Further study should be conducted to find the reason of inhibition of oxidation by Fe(VI).

  3. MIB-producing cyanobacteria (Planktothrix sp.) in a drinking water reservoir: distribution and odor producing potential.

    Science.gov (United States)

    Su, Ming; Yu, Jianwei; Zhang, Junzhi; Chen, Hui; An, Wei; Vogt, Rolf D; Andersen, Tom; Jia, Dongmin; Wang, Jingshi; Yang, Min

    2015-01-01

    The production of odorant 2-methylisoborneol (MIB) in water bodies by Planktothrix sp. have not been understood very well. Through a four-year investigation in Miyun Reservoir, a huge mesotrophic drinking water reservoir known to have the MIB episodes, we found that the Planktothrix sp. bloomed during September and October causing the high levels of MIB in the reservoir. The concentration of MIB and the biomass of MIB-producing cyanobacteria Planktothrix were measured (n = 887) at different sites and depths during different seasons. The results indicated that the shallow region of the reservoir is the major habitat for Planktothrix sp. due to that the light is able to penetrate down to the relatively high concentrations of nutrients close to the sediments. Quantile regression analysis between Planktothrix biomass and MIB concentration shows that the risk of MIB exceeding the odor threshold (15 ng L⁻¹) in water was as high as 90% when the Planktothrix density was more than 4.0 × 10⁵ cells L⁻¹, while the risk was reduced to 10% when the Planktothrix density remained below 1.6 × 10⁴ cells L⁻¹. This study will improve the understanding of the environmental behaviors of Planktothrix sp., and can provide useful information for better management of drinking water lakes/reservoirs experiencing the taste and odor (T&O) problems caused by deep living cyanobacterial species.

  4. PROGNOSTIC SIGNIFICANCE OF MIB1 PROLIFERATIONMARKER EXPRESSION ON DUCTAL CARCINOMA IN SITU ANDINVASIVE DUCTAL CARCINOMA OF THE BREAST

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Objective To determine the prognostic significance of MIB1 proliferation marker expression on ductal carci noma in situ (DCIS) and invasive ductal carcinoma (IDC) of the breast. Methods By means of histological and immunohis tochemical techniques, MIB1 labelling index (LI) was determined in 31 pathologic specimens (DICS 6, IDC 22 and 3 benign breast lesions as control). Results Significantly higher (P<0.05) MIB1 expression was noted in breast carcinomas in con trast with benign breast lesions. MIB1 LI increased with increasing tumor invasion from DCIS to IDC (P<0.05). Increased tendency of MIB1 LI had been also noted in according with the increased nuclear grading (NG) of IDC. Conclusion MIB1 ex pression can faithfully reflect the proliferative activity of the breast lesions, where the breast cancers with both high MIB1 LI and NG seem to have a poor prognosis.

  5. Removal of MIB and geosmin using granular activated carbon with and without MIEX pre-treatment.

    Science.gov (United States)

    Drikas, Mary; Dixon, Mike; Morran, Jim

    2009-12-01

    This study assessed the impact of MIEX pre-treatment, followed by either coagulation or microfiltration (MF), on the effectiveness of pilot granular activated carbon (GAC) filters for the removal of the taste and odour compounds, 2-methylisoborneol (MIB) and geosmin, from a surface drinking water source over a 2-year period. Complete removal of MIB and geosmin was achieved by all GAC filters for the first 10 months, suggesting that the available adsorption capacity was sufficient to compensate for differences in dissolved organic carbon (DOC) entering the GAC filters. Reduction of empty bed contact time (EBCT), in all but one GAC filter, resulted in breakthrough of spiked MIB and geosmin, with initial results inconclusive regarding the impact of MIEX pre-treatment. MIB and geosmin removal increased over the ensuing 12 months until complete removal of both MIB and geosmin was again achieved in all but one GAC filter, which had been pre-chlorinated. Autoclaving and washing the GAC filters had minimal impact on geosmin removal but reduced MIB removal by 30% in all but the pre-chlorinated filter, confirming that biodegradation impacted MIB removal. The impact of biodegradation was greater than any impact on GAC adsorption arising from DOC differences due to MIEX pre-treatment. It is not clear whether, at a lower initial EBCT, MIEX pre-treatment may have impacted on the adsorption capacity of the virgin GAC. The GAC filter maintained at the longer EBCT, which was also pre-chlorinated, completely removed MIB and geosmin for the period of the study, suggesting that the greater adsorption capacity was compensating for any decrease in biological degradation.

  6. MIB-1 labeling index, Ki-67, is an indicator of invasive intraductal papillary mucinous neoplasm

    OpenAIRE

    Shimura, Tatsuo; Kofunato, Yasuhide; OKADA, Ryo; Yashima, Rei; Okada, Koji; Araki, Kenichiro; Hosouchi, Yasuo; Kuwano, Hiroyuki; Takenoshita, Seiichi

    2016-01-01

    Despite strict criteria for the observation of intraductal papillary mucinous neoplasm (IPMN), it remains difficult to distinguish invasive IPMN from non-invasive IPMN. The aim of the present study was to identify an indicator of invasive IPMN. The present study retrospectively evaluated 53 patients (28 with non-invasive and 25 with invasive IPMN) who underwent resection of IPMN, and examined the usefulness of the MIB-1 labeling index as an indicator of invasive IPMN. The MIB-1 labeling index...

  7. Degradation mechanisms of geosmin and 2-MIB during UV photolysis and UV/chlorine reactions.

    Science.gov (United States)

    Kim, Tae-Kyoung; Moon, Bo-Ram; Kim, Taeyeon; Kim, Moon-Kyung; Zoh, Kyung-Duk

    2016-11-01

    We conducted chlorination, UV photolysis, and UV/chlorin reactions to investigate the intermediate formation and degradation mechanisms of geosmin and 2-methylisoborneol (2-MIB) in water. Chlorination hardly removed geosmin and 2-MIB, while the UV/chlorine reaction at 254 nm completely removed geosmin and 2-MIB within 40 min and 1 h, respectively, with lesser removals of both compounds during UV photolysis. The kinetics during both UV photolysis and UV/chlorine reactions followed a pseudo first-order reaction. Chloroform was found as a chlorinated intermediate during the UV/chlorine reaction of both geosmin and 2-MIB. The pH affected both the degradation and chloroform production during the UV/chlorine reaction. The open ring and dehydration intermediates identified during UV/chlorine reactions were 1,4-dimethyl-adamantane, and 1,3-dimethyl-adamantane from geosmin, 2-methylenebornane, and 2-methyl-2-bornene from 2-MIB, respectively. Additionally, 2-methyl-3-pentanol, 2,4-dimethyl-1-heptene, 4-methyl-2-heptanone, and 1,1-dichloro-2,4-dimethyl-1-heptane were newly identified intermediates from UV/chlorine reactions of both geosmin and 2-MIB. These intermediates were degraded as the reaction progressed. We proposed possible degradation pathways during the UV photolysis and UV/chlorine reactions of both compounds using the identified intermediates.

  8. Study of AgNOR Value and MIB-1 in Breast Cancer Treated With Surgery

    Directory of Open Access Journals (Sweden)

    Iin Kurnia

    2012-09-01

    Full Text Available AgNOR and MIB-1 are marker for breast cancer cell proliferation and can be use as based for radiotherapy treatment after surgery. Value of AgNOR and MIB-1 index were determined using staining and immunohistochemistry staining method respectively from 25 of microscopic slides of breast cancer tissue patients with surgery, and grouped based on degree of differentiation, 3 slides were good degree (G1, 16 slides were medium degree (G2 and 6 slides were poor degree (between G2 and G3. The result shown that the value of AgNOR and MIB-1 index were tended to increase with the increased differentiation degree. There was a positive correlation between the value of AgNOR and index of MIB-1 in all group of differentiation degree (r = 0.21, there is a negative correlation between AgNOR and MIB-1 on G1 (r =-0,97, positive correlation in G2 (r = 0.36 as well as positive correlation between G2 and G3 (r = 0.33. The positive correlation between AgNOR and MIB-1 were associated to the increased of G1, S and G2 phase in the proliferation cell and an increase of cells undergoing mitosis. The negative correlation were caused by the different cell proportion in G1, S and G2 phase, and undergoing mitotis.

  9. Personality Types and Megabytes: Student Attitudes toward Computer Mediated Communication (CMC) in the Language Classroom.

    Science.gov (United States)

    Beauvois, Margaret Healy; Eledge, Jean

    1996-01-01

    Describes the results of a pilot study to examine the attitudes of university students toward the use of computer-mediated communication in their French conversation and composition course. Results indicate that both introvert and extrovert personality types generally perceive the use of a local area network as a beneficial experience. (15…

  10. A decoding method of an n length binary BCH code through (n + 1n length binary cyclic code

    Directory of Open Access Journals (Sweden)

    TARIQ SHAH

    2013-09-01

    Full Text Available For a given binary BCH code Cn of length n = 2 s - 1 generated by a polynomial of degree r there is no binary BCH code of length (n + 1n generated by a generalized polynomial of degree 2r. However, it does exist a binary cyclic code C (n+1n of length (n + 1n such that the binary BCH code Cn is embedded in C (n+1n . Accordingly a high code rate is attained through a binary cyclic code C (n+1n for a binary BCH code Cn . Furthermore, an algorithm proposed facilitates in a decoding of a binary BCH code Cn through the decoding of a binary cyclic code C (n+1n , while the codes Cn and C (n+1n have the same minimum hamming distance.

  11. Discovering Topology of MultiSubnet LAN using MIB

    Directory of Open Access Journals (Sweden)

    Sapna Harishankar

    2012-08-01

    Full Text Available Today, Ethernet is the dominant local area network(LAN technology. These networks, typically, comprise large number of elements from different vendors. This raises considerable difficulties in performing network management tasks, such as resource management and root cause analysis, which are practically impossible without an up-to-date knowledge of the physical network topology. Given the dynamic nature of today's LANs, keeping track of topology information manually is a daunting (if not impossible task. Therefore, it is essential to develop practical schemes for automatic inference of the physical topology of Ethernet networks. In this paper, we propose a simple and efficient algorithmic solution for discovering the physical topology of large, heterogeneous Ethernet LANs that may include multiple subnets as well as uncooperative network elements, like hubs. Our scheme utilizes only generic MIB information and does not required any hardware or software modification of the underlying network elements. By rigorous analysis, we prove that our method correctly infers the network topology and has low communication and computational overheads.

  12. Rate-adaptive BCH coding for Slepian-Wolf coding of highly correlated sources

    DEFF Research Database (Denmark)

    Forchhammer, Søren; Salmistraro, Matteo; Larsen, Knud J.

    2012-01-01

    This paper considers using BCH codes for distributed source coding using feedback. The focus is on coding using short block lengths for a binary source, X, having a high correlation between each symbol to be coded and a side information, Y, such that the marginal probability of each symbol, Xi in...

  13. Fundamental uncertainty in the BAO scale from isocurvature modes

    Energy Technology Data Exchange (ETDEWEB)

    Zunckel, C., E-mail: caroline.zunckel@gmail.co [Astrophysics and Cosmology Research Unit, School of Mathematical Sciences, University of Kwazulu-Natal, Durban 4041 (South Africa); Astrophysics Department, Princeton University, Peyton Hall, 4 Ivy Lane, NJ 08544 (United States); Okouma, P. [Department of Maths and Applied Maths, University of Cape Town, Rondebosch 7701, Cape Town (South Africa); Centre for High Performance Computing, CSIR Campus, 15 Lower Hope St., Rosebank, Cape Town (South Africa); South African Astronomical Observatory, Observatory, Cape Town (South Africa); African Institute for Mathematical Sciences, 6-8 Melrose Road, Muizenberg, Cape Town (South Africa); Muya Kasanda, S.; Moodley, K. [Astrophysics and Cosmology Research Unit, School of Mathematical Sciences, University of Kwazulu-Natal, Durban 4041 (South Africa); Centre for High Performance Computing, CSIR Campus, 15 Lower Hope St., Rosebank, Cape Town (South Africa); Bassett, B.A. [Department of Maths and Applied Maths, University of Cape Town, Rondebosch 7701, Cape Town (South Africa); Centre for High Performance Computing, CSIR Campus, 15 Lower Hope St., Rosebank, Cape Town (South Africa); South African Astronomical Observatory, Observatory, Cape Town (South Africa); African Institute for Mathematical Sciences, 6-8 Melrose Road, Muizenberg, Cape Town (South Africa)

    2011-02-14

    Small fractions of isocurvature perturbations correlated with the dominant adiabatic mode are shown to be a significant primordial systematic for Baryon Acoustic Oscillation (BAO) surveys which must be accounted for in future surveys. Isocurvature modes distort the standard ruler distance by broadening and shifting the peak in the galaxy correlation function. While a single isocurvature mode does not significantly degrade dark energy constraints, the general case with multiple isocurvature modes leads to biases that exceed 7{sigma} on average in the dark energy parameters even for isocurvature amplitudes undetectable by PLANCK. Accounting for all isocurvature modes corrects for this bias but degrades the dark energy figure of merit by at least 50% in the case of the BOSS experiment. However the BAO data in turn provides significantly stronger constraints on the nature of the primordial perturbations. Future large galaxy surveys will thus be powerful probes of exotic physics in the early Universe in addition to helping pin down the nature of dark energy.

  14. Factors affecting the removal of geosmin and MIB in drinking water biofilters

    Energy Technology Data Exchange (ETDEWEB)

    Elhadi, S.L.N.; Huck, P.M.; Slawson, R.M. [Wilfrid Laurier University, Waterloo, ON (Canada). Dept. for Biology

    2006-08-15

    Bench-scale experiments were conducted using four parallel dual-media filter columns containing biologically active anthracite or granular activated carbon media and sand. The factors under investigation were low-(8{sup o}C) and high-(20{sup o}C) temperature operations, geosmin and 2-methylisoborneol (MIB) concentration, media type, and biodegradable organic matter (BOM) level. Source water consisted of dechlorinated tap water to which geosmin and MIB were added, as well as a cocktail of easily biodegradable organic matter (i.e., typical ozonation by-products). Phase 1 experiments used a high BOM level (280 {mu} g/L carbon) to simulate water that had been subjected to ozonation before filtration. Phase 2 experiments used a low BOM level (28 {mu} g/L C) to simulate nonozonated water. Factorial design experiments showed that all four main factors (temperature, concentration, media, and BOM level) were important to both geosmin and MIB removal. Temperature and media interaction and concentration and BOM level interaction were significant for geosmin removal only. Temperature and BOM level interaction as well as media and BOM level interaction were significant for the removal of both geosmin and MIB. Overall, removals of geosmin and MIB were lower in phase 2 (low BOM level), in particular in the anthracite media filters. Biomass levels in the filters appeared to have a significant effect upon the removal efficiencies of both odor compounds.

  15. Biodegradation rates of 2-methylisoborneol (MIB) and geosmin through sand filters and in bioreactors.

    Science.gov (United States)

    Ho, Lionel; Hoefel, Daniel; Bock, Franziska; Saint, Christopher P; Newcombe, Gayle

    2007-02-01

    Taste and odour (T&O) causing compounds, in particular, 2-methylisoborneol (MIB) and geosmin, are a problem for water authorities as they are recalcitrant to conventional water treatment. In this study, biological sand filtration was shown to be an effective process for the complete removal of MIB and geosmin, with removal shown to be predominantly through biodegradation. In addition, MIB and geosmin were also effectively degraded in batch bioreactor experiments using biofilm sourced from one of the sand filters as the microbial inoculum. The biodegradation of MIB and geosmin was determined to be a pseudo-first-order reaction with rate constants ranging between 0.10 and 0.58 d(-1) in the bioreactor experiments. Rate constants were shown to be dependent upon the initial concentration of the microbial inoculum but not the initial concentration of MIB and geosmin when target concentrations of 200 and 50 ng l(-1) were used. Furthermore, rate constants were shown to increase upon re-exposure of the biofilm to both T&O compounds. Enrichment cultures with subsequent community profile analysis using 16S rRNA-directed PCR-DGGE identified four bacteria most likely involved in the biodegradation of geosmin within the sand filters and bioreactors. These included a Pseudomonas sp., Alphaproteobacterium, Sphingomonas sp. and an Acidobacteriaceae member.

  16. Geosmin and MIB events in a new reservoir in southern California.

    Science.gov (United States)

    Izaguirre, G; Taylor, W D

    2007-01-01

    Diamond Valley Lake is a large drinking water reservoir in western Riverside County, California near the city of Hemet. In almost 6 years since filling began in 1999, this reservoir has experienced five episodes involving either geosmin or 2-methylisoborneol (MIB). The first one was a short-lived but intense geosmin event in May 2000, associated with a planktonic cyanobacterium, Anabaena circinalis. Geosmin levels reached 750 ng/L at the surface. All the other episodes involved benthic proliferations in the littoral zone. In September 2002, an MIB-producing growth developed in a shallow area near the outlet tower, dominated by Oscillatoria cf. curviceps and O. limosa. A similar event occurred in October 2003. In March 2004, an extensive growth of cyanobacteria that included two geosmin producers developed at the east dam, but had minimal effect on geosmin levels in the water. Finally, there was a major MIB episode in October 2004, in which the primary causative organism was again Oscillatoria cf. curviceps. A band of benthic cyanobacteria developed all around the shoreline from 3-9 m deep, and surface MIB levels reached 63 ng/L. These events showed that a new reservoir in a mild climate can be colonised by benthic cyanobacteria that produce MIB and geosmin, within a relatively short time.

  17. Removal of geosmin and MIB by biofiltration--an investigation discriminating between adsorption and biodegradation.

    Science.gov (United States)

    Persson, F; Heinicke, G; Hedberg, T; Hermansson, M; Uhl, W

    2007-01-01

    Geosmin and 2-methylisoborneol (MIB) are two substances causing earthy/musty odours that are difficult to remove by conventional chemical drinking water treatment. In this study removal of geosmin and MIB by biofiltration of untreated surface water was investigated using granular activated carbon (GAC) and crushed expanded clay (EC) as filter media. Biofiltration through both GAC and EC removed geosmin and MIB present at low (20 ng l(-1)) concentrations by at least 97% at an empty bed contact time of 30 minutes and a temperature of 15 degrees C. At lower temperature (6-12 degrees C) and simultaneously lower biomass concentrations, removal efficiency was similar in the GAC but considerably lower in the EC biofilter, pointing to a second mechanism different from biodegradation. Consequently, microbial activity was suppressed with azide to enable discrimination between biodegradation and adsorption. During azide dosage, the GAC biofilters still removed geosmin and MIB nearly unaffectedly. In the EC biofilter, however, removal of both odorants ceased completely. Methylene blue adsorption confirmed that the GAC, even after almost four years of operation receiving surface water, had capacity to remove geosmin and MIB by adsorption. Since odour episodes commonly occur during the warm season when microbiological activity is high, EC constitutes a viable option as carrier medium for direct biological filtration of surface water. The additional GAC adsorption capacity however adds robustness to the removal process.

  18. ER, p53 and MIB-1 are significantly associated with malignant phyllodes tumor

    Directory of Open Access Journals (Sweden)

    Nurhayati H Munawer

    2012-12-01

    Full Text Available Background: Phyllodes tumors (PT are rare. We evaluated the expression status of ER, Bcl2, p53, and MIB-1 protein in these tumors. Methods: One hundred and ninety-three tumors were examined using immunohistochemistry on tissue microarray. Results: ERβ (p <0.001, and p53 (p=0.006 in the stromal component were associated with tumor size. p53 expression was significantly associated with both epithelial and stro­mal components of malignant PTs (p<0.05. In PT, the decreased expressions of p53 and MIB-1 were significantly different with positive Bcl2 protein expression in epi­thelial component (p=0.000. Besides, MIB-1 was also found to be associated with ERα and ERβ in stromal component (p=0.000. Conclusion: The expression of p53 with tumor size and histological grade in PTs may increase risk for malignancy.

  19. Stereochemical conversion of C3-vinyl group to 1-hydroxyethyl group in bacteriochlorophyll c by the hydratases BchF and BchV: adaptation of green sulfur bacteria to limited-light environments.

    Science.gov (United States)

    Harada, Jiro; Teramura, Misato; Mizoguchi, Tadashi; Tsukatani, Yusuke; Yamamoto, Ken; Tamiaki, Hitoshi

    2015-12-01

    Photosynthetic green sulfur bacteria inhabit anaerobic environments with very low-light conditions. To adapt to such environments, these bacteria have evolved efficient light-harvesting antenna complexes called as chlorosomes, which comprise self-aggregated bacteriochlorophyll c in the model green sulfur, bacterium Chlorobaculum tepidum. The pigment possess a hydroxy group at the C3(1) position that produces a chiral center with R- or S-stereochemistry and the C3(1) -hydroxy group serves as a connecting moiety for the self-aggregation. Chlorobaculum tepidum carries the two possible homologous genes for C3-vinyl hydratase, bchF and bchV. In the present study, we constructed deletion mutants of each of these genes. Pigment analyses of the bchF-inactivated mutant, which still has BchV as a sole hydratase, showed higher ratios of S-epimeric bacteriochlorophyll c than the wild-type strain. The heightened prevalence of S-stereoisomers in the mutant was more remarkable at lower light intensities and caused a red shift of the chlorosomal Qy absorption band leading to advantages for light-energy transfer. In contrast, the bchV-mutant possessing only BchF showed a significant decrease of the S-epimers and accumulations of C3-vinyl BChl c species. As trans- criptional level of bchV was upregulated at lower light intensity, the Chlorobaculum tepidum adapted to low-light environments by control of the bchV transcription. © 2015 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd.

  20. A Very Low Cost BCH Decoder for High Immunity of On-Chip Memories

    Science.gov (United States)

    Seo, Haejun; Han, Sehwan; Heo, Yoonseok; Cho, Taewon

    BCH(Bose-Chaudhuri-Hoquenbhem) code, a type of block codes-cyclic codes, has very strong error-correcting ability which is vital for performing the error protection on the memory system. BCH code has many kinds of dual algorithms, PGZ(Pererson-Gorenstein-Zierler) algorithm out of them is advantageous in view of correcting the errors through the simple calculation in t value. However, this is problematic when this becomes 0 (divided by zero) in case ν ≠ t. In this paper, the circuit would be simplified by suggesting the multi-mode hardware architecture in preparation that v were 0~3. First, production cost would be less thanks to the smaller number of gates. Second, lessening power consumption could lengthen the recharging period. The very low cost and simple datapath make our design a good choice in small-footprint SoC(System on Chip) as ECC(Error Correction Code/Circuit) in memory system.

  1. Interference of iron as a coagulant on MIB removal by powdered activated carbon adsorption for low turbidity waters.

    Science.gov (United States)

    Seckler, Ferreira Filho Sidney; Margarida, Marchetto; Rosemeire, Alves Laganaro

    2013-08-01

    Powered activated carbon (PAC) is widely used in water treatment plants to minimize odors in drinking water. This study investigated the removal of 2-methylisoborneol (MIB) by PAC adsorption, combined with coagulation using iron as a coagulant. The adsorption and coagulation process were studied through different case scenarios of jar tests. The analysis evaluated the effect of PAC dosing in the liquid phase immediately before or after the coagulant addition. Ferric sulphate was used as the coagulant with dosages from 10 to 30 mg/L, and PAC dosages varied from 10 to 40 mg/L. The highest MIB removal efficiency (about 70%) was achieved without the coagulant addition and with the highest PAC dosage (40 mg/L). Lower MIB removal efficiencies were observed in the presence of coagulant, showing a clear interference of the iron precipitate or coagulant in the adsorption process. The degree of interference of the coagulation process in the MIB removal was proportional to the ratio of ferric hydroxide mass to the PAC mass. For both cases of PAC dosing, upstream and downstream of the coagulant injection point, the MIB removal efficiency was similar. However, MIB removal efficiency was 15% lower when compared with experiments without the coagulant application. This interference in the MIB adsorption occurs potentially because the coagulant coats the surface of the carbon and interferes with the MIB coming in contact with the carbon's surface and pores. This constraint requires an increase of the PAC dosage to provide the same efficiency observed without coagulation.

  2. Report from the Melanoma Independent Board First Melanoma MIB Conference, 21-22 October 2013.

    Science.gov (United States)

    Testori, A; Ascierto, P; Chiarion Sileni, V; De Lorenzo, F; Pelicci, Pg; Rossi, Cr

    2014-01-01

    The Melanoma Independent Board (MIB) held its first conference from 21 to 22 October, 2013, in Rome, Italy. Like the MIB itself, the conference brought together specialists from all aspects of cancer care: doctors, patient associations, journalists, and representatives from local government, hospitals, and pharma to encourage an interdisciplinary discussion on the future of melanoma. It was hoped that the conference would be an opportunity for all participants to see and understand each other's points of view. In memoriam of melanoma pioneer Natalie Cascinelli, the conference focussed on innovation and sustainability as well as the latest drug developments.

  3. Synthesis of cis-(±)-2-Hydroxymethyl-5-(Cytosine-1'-yl)- 1,3-Oxathiolane (BCH-189)

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    The target compound BCH-189 was synthesized with high yield via four steps from benzoyloxy acetylaldehyde and p-dithiane-2,5-diol as starting materials. The synthetic route is preferable to what literature reported.

  4. Observer reliability in assessment of mitotic activity and MIB-1-determined proliferation rate in pediatric sarcomas

    NARCIS (Netherlands)

    Molenaar, W M; Plaat, B E; Berends, E R; te Meerman, G J

    2000-01-01

    In hematoxylin-eosin-stained sections of 20 pediatric sarcomas the mitotic index was assessed by four experienced pathologists and four less-experienced observers without prior instructions. In adjacent sections immunolabeled for MIB-1, the proliferation index was assessed as the estimated percentag

  5. Medulloblastoma: evaluation of proliferative index by monoclonal antibody Mib-1, its prognostic correlation and therapeutic implications

    Directory of Open Access Journals (Sweden)

    Ferrari Antonio Fernandes

    2003-01-01

    Full Text Available In the past few years, the monoclonal antibody MIB-1 has been used by researchers in order to retrospectively study paraffin imbibed tumor fragments. The medulloblastoma is the most common malignant central nervous system tumor in childhood. The objectives were: determination of the mean Mib-1 LI value from these patients, as well as the prognostic value of the method.This retrospective study represents an analysis of the cellular proliferation index of posterior fossa medulloblastomas collected from 22 patients at A.C. Camargo Hospital, from January 1990 to December 1999. The histopathological diagnosis was confirmed by H&E and proliferative index (LI was achived with Mib-1 which detects proliferating cells during G1, G2, S and M phases.The results demostrated that the mean Mib-1 was 30,1%, and ranged from 5,2% to 62,0%.In conclusion, this method has prognostic value, has to be used as routine for patients harboring medulloblastomas and the ones who have PI greater than the mean value found in this study, should be treated aggressively.

  6. Cosmology with Phase Statistics: Parameter Forecasts and Detectability of BAO

    CERN Document Server

    Eggemeier, Alexander

    2016-01-01

    We consider an alternative to conventional three-point statistics such as the bispectrum, which is purely based on the Fourier phases of the density field: the line correlation function. This statistic directly probes the non-linear clustering regime and contains information highly complementary to that contained in the power spectrum. In this work, we determine, for the first time, its potential to constrain cosmological parameters and detect baryon acoustic oscillations (hereafter BAOs). We show how to compute the line correlation function for a discrete sampled set of tracers that follow a local Lagrangian biasing scheme and demonstrate how it breaks the degeneracy between the amplitude of density fluctuations and the bias parameters of the model. We then derive analytic expressions for its covariance and show that it can be written as a sum of a Gaussian piece plus non-Gaussian corrections. We compare our predictions with a large ensemble of $N$-body simulations and confirm that BAOs do indeed modulate th...

  7. Alcock-Paczynski Test with Model-independent BAO Data

    CERN Document Server

    Melia, Fulvio

    2015-01-01

    Cosmological tests based on the statistical analysis of galaxy distributions are usually dependent on the evolution of the sources. An exception is the Alcock-Paczynski (AP) test, which is based on the changing ratio of angular to spatial/redshift size of (presumed) spherically-symmetric source distributions with distance. Intrinsic redshift distortions due to gravitational effects may also have an influence, but there is now a way to overcome them: with the inclusion in the AP test of an observational signature with a sharp feature, such as the Baryonic Acoustic Oscillation (BAO) peak. Redshift distortions affect only the amplitude of the peak, not its position. As we will show here, the use of this diagnostic, with newly acquired data on the anisotropic distribution of the BAO peaks from SDSS-III/BOSS-DR11 at average redshifts 0.57 and 2.34, strongly disfavours the current concordance (LCDM) model, which is discarded at the 3-sigma level. A statistically acceptable fit to the AP data with wCDM (the version ...

  8. BAO Plate Archive Project: Digitization, Electronic Database and Research Programmes

    Science.gov (United States)

    Mickaelian, A. M.; Abrahamyan, H. V.; Andreasyan, H. R.; Azatyan, N. M.; Farmanyan, S. V.; Gigoyan, K. S.; Gyulzadyan, M. V.; Khachatryan, K. G.; Knyazyan, A. V.; Kostandyan, G. R.; Mikayelyan, G. A.; Nikoghosyan, E. H.; Paronyan, G. M.; Vardanyan, A. V.

    2016-06-01

    The most important part of the astronomical observational heritage are astronomical plate archives created on the basis of numerous observations at many observatories. Byurakan Astrophysical Observatory (BAO) plate archive consists of 37,000 photographic plates and films, obtained at 2.6m telescope, 1m and 0.5m Schmidt type and other smaller telescopes during 1947-1991. In 2002-2005, the famous Markarian Survey (also called First Byurakan Survey, FBS) 1874 plates were digitized and the Digitized FBS (DFBS) was created. New science projects have been conducted based on these low-dispersion spectroscopic material. A large project on the whole BAO Plate Archive digitization, creation of electronic database and its scientific usage was started in 2015. A Science Program Board is created to evaluate the observing material, to investigate new possibilities and to propose new projects based on the combined usage of these observations together with other world databases. The Executing Team consists of 11 astronomers and 2 computer scientists and will use 2 EPSON Perfection V750 Pro scanners for the digitization, as well as Armenian Virtual Observatory (ArVO) database will be used to accommodate all new data. The project will run during 3 years in 2015-2017 and the final result will be an electronic database and online interactive sky map to be used for further research projects, mainly including high proper motion stars, variable objects and Solar System bodies.

  9. Large-scale BAO signatures of the smallest galaxies

    Energy Technology Data Exchange (ETDEWEB)

    Dalal, Neal; Pen, Ue-Li [Canadian Institute for Theoretical Astrophyics, University of Toronto, 60 St. George St., Toronto, Ontario M5S 3H8 (Canada); Seljak, Uros, E-mail: neal@cita.utoronto.ca, E-mail: pen@cita.utoronto.ca, E-mail: useljak@berkeley.edu [Physics Department and Lawrence Berkeley National Laboratory, University of California, Berkeley, California 94720 (United States)

    2010-11-01

    Recent work has shown that at high redshift, the relative velocity between dark matter and baryonic gas is typically supersonic. This relative velocity suppresses the formation of the earliest baryonic structures like minihalos, and the suppression is modulated on large scales. This effect imprints a characteristic shape in the clustering power spectrum of the earliest structures, with significant power on ∼ 100 Mpc scales featuring highly pronounced baryon acoustic oscillations. The amplitude of these oscillations is orders of magnitude larger at z ∼ 20 than previously expected. This characteristic signature can allow us to distinguish the effects of minihalos on intergalactic gas at times preceding and during reionization. We illustrate this effect with the example of 21 cm emission and absorption from redshifts during and before reionization. This effect can potentially allow us to probe physics on kpc scales using observations on 100 Mpc scales. We present sensitivity forecasts for FAST and Arecibo. Depending on parameters, this enhanced structure may be detectable by Arecibo at z ∼ 15−20, and with appropriate instrumentation FAST could measure the BAO power spectrum with high precision. In principle, this effect could also pose a serious challenge for efforts to constrain dark energy using observations of the BAO feature at low redshift.

  10. BCH-代数的(s,t]-模糊H-理想%(s,t]-fuzzy H-ideals of BCH-algebras

    Institute of Scientific and Technical Information of China (English)

    彭家寅

    2010-01-01

    利用模糊点与模糊集的关系,引入了BCH-代数的(∈,∈∨ q)-模糊H-理想、((-∈),(-∈)∨ (-q))-模糊H-理想和(s,t]-模糊H-理想的概念,指出了Hu-模糊H-理想、(∈,∈∨q)-模糊H-理想和((-∈),(-∈)∨ (-q))-模糊H-理想均为(s,t]-模糊H-理想的特例,讨论它们与其相应的水平H-理想的关系.(s,t]-模糊H-理想的同态象和同态原象是否成为(s,t]-模糊H-理想被研究,(s,t]-模糊H-理想的交与笛卡儿积被讨论.

  11. Stimulation of 2-methylisoborneol (MIB) production by actinomycetes after cyclic chlorination in drinking water distribution systems.

    Science.gov (United States)

    Abbaszadegan, Morteza; Yi, Min; Alum, Absar

    2015-01-01

    The impact of fluctuation in chlorine residual on actinomycetes and the production of 2-methylisoborneol (MIB) were studied in cast-iron and PVC model distribution systems. Actinomycetes were spiked in each system and continued operation for a 12-day non-chlorine experiment, resulting in no changes in actinomycetes and MIB concentrations. Three cyclic chlorination events were performed and chlorine residuals were maintained as follows: 1.0 mg L(-1) for 24 h, 0 mg L(-1) for 48 h, 0.5 mg L(-1) for 48 h, 0 mg L(-1) for 48 h and 2 mg L(-1) for 24 h. After each chlorination event, 2 -3 log decrease in actinomycetes was noted in both systems. However, within 48 h at 0 mg L(-1) chlorine, the actinomycetes recovered to the pre-chlorination levels. On the contrary, MIB concentration in both systems remained un-impacted after the first cycle and increased by fourfold ( 20 mg L(-1)) after the second cycle, which lasted through the third cycle despite the fact that actinomycetes numbers fluctuated 2-3 logs during this time period. For obtaining biofilm samples from field, water meters were collected from municipality drinking water distribution systems located in central Arizona. The actinomycetes concentration in asbestos cement pipe and cast iron pipe averaged 3.1 × 10(3) and 1.9 × 10(4) CFU cm(-2), respectively. The study shows that production of MIB is associated with changes in chlorine residual in the systems. This is the first report of cyclic chlorine shock as a stimulus for MIB production by actinomycetes in drinking water distribution system's ecology.

  12. Clinicopathological significance of p16, cyclin D1, Rb and MIB-1 levels in skull base chordoma and chondrosarcoma

    Institute of Scientific and Technical Information of China (English)

    Jun-qi Liu; Qiu-hang Zhang; Zhen-lin Wang

    2015-01-01

    Objective: To investigate the expression of p16, cyclin D1, retinoblastoma tumor suppressor protein (Rb) and MIB-1 in skull base chordoma and chondrosarcoma tissues, and to determine the clinicopathological significance of the above indexes in these diseases.Methods: A total of 100 skull base chordoma, 30 chondrosarcoma, and 20 normal cartilage tissue samples were analyzed by immunohistochemistry.The expression levels of p16, cyclinD1,Rb and MIB-1 proteins were assessed for potential correlation with the clinicopathological features.Results: As compared to normal cartilage specimen (control), there was decreased expression of p16, and increased expression of cyclin D1, Rb and MIB-1 proteins, in both skull base chordoma and chondrosarcoma specimens.MIB-1 LI levels were significantly increased in skull base chordoma specimens with negative expression of p16, and positive expression of cyclin D1 and Rb (P < 0.05).Significantly elevated MIB-1 LI was also detected in skull base chondrosarcoma tissues, while there was negative expression of p16, cyclin D1 and Rb (P < 0.05).In skull base chordoma, p16 negatively correlated with cyclin D1 and Rb, while cyclin D1 positively correlated with Rb.Additionally, p16, cyclin D1, Rb, or MIB-1 expression showed no correlation with age, gender, or pathological classification of patients with skull base chordoma (P > 0.05).However, p16 and MIB-1 levels correlated with the intradural invasion, and expression of p16, Rb and MIB-1 correlated with the number of tumor foci (P < 0.05).Further, the expression of p16 and MIB-1 appeared to correlate with the prognosis of patients with skull base chordoma.Conclusions: The abnormal expression of p16, cyclin D1 and Rb proteins might be associated with the tumorigenesis of skull base chordoma and chondrosarcoma.

  13. Proliferative activity (MIB-1 index) is an independent prognostic parameter in patients with high-grade soft tissue sarcomas of subtypes other than malignant fibrous histiocytomas

    DEFF Research Database (Denmark)

    Jensen, V; Sørensen, Flemming Brandt; Bentzen, S M

    1998-01-01

    . The proliferative activity was assessed by use of the monoclonal antibody MIB-1 and evaluated in multiple, random systematic sampled fields of vision. The percentage of proliferating cells (the MIB-1 index) ranged between 1% and 85% (median 12%). A significant increase in mean MIB-1 index was seen with increasing....... Univariate analysis identified patient age, tumour size, histological grade of malignancy, MIB-1 index and p53 accumulation as significant prognostic parameters. Multivariate Cox analysis, including tests for interaction terms between histological subtypes and MIB-1 index, showed independent prognostic...... effect of MIB-1 index and tumour size in patients with high-grade tumours of other subtypes than malignant fibrous histiocytoma (MFH). CONCLUSIONS: Histopathological malignancy grading is the most important single prognostic factor for overall survival in STS, but estimation of MIB-1 index is useful...

  14. Association between in vivo iododeoxyuridine labeling, MIB-1 expression, malignancy grade and clinical stage in human prostate cancer

    DEFF Research Database (Denmark)

    Borre, M; Høyer, M; Sørensen, Flemming Brandt

    1998-01-01

    labeled in vivo with IdUrd (a thymidine analogue incorporated into DNA in S-phase cells) by intravenous infusion before transurethral resection. IdUrd-labeled cells and MIB-1-positive cells were detected by immunohistochemistry. We found statistically significant associations between the tumor cell...... proliferation rates measured by in vivo IdUrd labeling and MIB-1 expression in formalin-fixed paraffin-embedded tumors. Good correlations were also found between S-phase fraction, MIB-1 expression, clinical stage and malignancy grade. These results make larger retrospective studies on archival tissue meaningful....

  15. Functional divergence outlines the evolution of novel protein function in NifH/BchL protein family

    Indian Academy of Sciences (India)

    Subarna Thakur; Asim K Bothra; Arnab Sen

    2013-11-01

    Biological nitrogen fixation is accomplished by prokaryotes through the catalytic action of complex metalloenzyme, nitrogenase. Nitrogenase is a two-protein component system comprising MoFe protein (NifD&K) and Fe protein (NifH). NifH shares structural and mechanistic similarities as well as evolutionary relationships with light-independent protochlorophyllide reductase (BchL), a photosynthesis-related metalloenzyme belonging to the same protein family. We performed a comprehensive bioinformatics analysis of the NifH/BchL family in order to elucidate the intrinsic functional diversity and the underlying evolutionary mechanism among the members. To analyse functional divergence in the NifH/BchL family, we have conducted pair-wise estimation in altered evolutionary rates between the member proteins. We identified a number of vital amino acid sites which contribute to predicted functional diversity.We have also made use of the maximum likelihood tests for detection of positive selection at the amino acid level followed by the structure-based phylogenetic approach to draw conclusion on the ancient lineage and novel characterization of the NifH/BchL protein family. Our investigation provides ample support to the fact that NifH protein and BchL share robust structural similarities and have probably deviated from a common ancestor followed by divergence in functional properties possibly due to gene duplication.

  16. Testing averaged cosmology with type Ia supernovae and BAO data

    CERN Document Server

    Santos, B; Devi, N Chandrachani; Alcaniz, J S

    2016-01-01

    An important problem in precision cosmology is the determination of the effects of averaging and backreaction on observational predictions, particularly in view of the wealth of new observational data and improved statistical techniques. In this paper we discuss the observational viability of a class of averaged cosmologies which consist of a simple parametrized phenomenological two-scale backreaction model with decoupled spatial curvature parameters. We perform a Bayesian model selection analysis and find that this class of averaged phenomenological cosmological models is favored with respect to the standard $\\Lambda$CDM cosmological scenario when a joint analysis of current SNe Ia and BAO data is performed. In particular, the analysis provides observational evidence for non-trivial spatial curvature.

  17. Cosmology with phase statistics: parameter forecasts and detectability of BAO

    Science.gov (United States)

    Eggemeier, Alexander; Smith, Robert E.

    2017-04-01

    We consider an alternative to conventional three-point statistics such as the bispectrum, which is purely based on the Fourier phases of the density field: the line correlation function. This statistic directly probes the non-linear clustering regime and contains information highly complementary to that contained in the power spectrum. In this work, we determine, for the first time, its potential to constrain cosmological parameters and detect baryon acoustic oscillations (hereafter BAOs). We show how to compute the line correlation function for a discrete sampled set of tracers that follow a local Lagrangian biasing scheme and demonstrate how it breaks the degeneracy between the amplitude of density fluctuations and the bias parameters of the model. We then derive analytic expressions for its covariance and show that it can be written as a sum of a Gaussian piece plus non-Gaussian corrections. We compare our predictions with a large ensemble of N-body simulations and confirm that BAOs do indeed modulate the signal of the line correlation function for scales 50-100 h-1Mpc and that the characteristic S-shape feature would be detectable in upcoming Stage IV surveys at the level of ∼4σ. We then focus on the cosmological information content and compute Fisher forecasts for an idealized Stage III galaxy redshift survey of volume V ∼ 10 h-3 Gpc3 and out to z = 1. We show that combining the line correlation function with the galaxy power spectrum and a Planck-like microwave background survey yields improvements up to a factor of 2 for parameters such as σ8, b1 and b2, compared with using only the two-point information alone.

  18. A Low-Complexity and High-Throughput RTL Design of a BCH (15,7 Decoder

    Directory of Open Access Journals (Sweden)

    Hendra Setiawan

    2012-09-01

    Full Text Available The Bose, Chaudhuri and Hocquenghem (BCH codes form a large class of powerful random-error correcting cyclic codes. However, the implementation of its decoder requires high-complexity computation resources with a huge number of sequential circuits. This paper presents a low-complexity register transfer level (RTL circuit design of a BCH decoder. In accordance with the table relationship between the syndrome and the error bit position, we propose a circuit that is mostly occupied by combinational elements without any sequential evolvement. Therefore the designed system has a low complexity and high throughput properties. The implementation of the BCH (15,7decoder on Virtex 5 FX70TFF1136 requires 77 look-up tables (LUTs with the maximum throughput reaching 1.7 Gbps.

  19. Information-Dispersion-Entropy-Based Blind Recognition of Binary BCH Codes in Soft Decision Situations

    Directory of Open Access Journals (Sweden)

    Yimeng Zhang

    2013-05-01

    Full Text Available A method of blind recognition of the coding parameters for binary Bose-Chaudhuri-Hocquenghem (BCH codes is proposed in this paper. We consider an intelligent communication receiver which can blindly recognize the coding parameters of the received data stream. The only knowledge is that the stream is encoded using binary BCH codes, while the coding parameters are unknown. The problem can be addressed on the context of the non-cooperative communications or adaptive coding and modulations (ACM for cognitive radio networks. The recognition processing includes two major procedures: code length estimation and generator polynomial reconstruction. A hard decision method has been proposed in a previous literature. In this paper we propose the recognition approach in soft decision situations with Binary-Phase-Shift-Key modulations and Additive-White-Gaussian-Noise (AWGN channels. The code length is estimated by maximizing the root information dispersion entropy function. And then we search for the code roots to reconstruct the primitive and generator polynomials. By utilizing the soft output of the channel, the recognition performance is improved and the simulations show the efficiency of the proposed algorithm.

  20. Interference of iron as a coagulant on MIB removal by powdered activated carbon adsorption for low turbidity waters

    Institute of Scientific and Technical Information of China (English)

    Ferreira Filho SIDNEY SECKLER; Marchetto MARGARIDA; Alves Laganaro ROSEMEIRE

    2013-01-01

    Powered activated carbon (PAC) is widely used in water treatment plants to minimize odors in drinking water.This study investigated the removal of 2-methylisoborneol (MIB) by PAC adsorption,combined with coagulation using iron as a coagulant.The adsorption and coagulation process were studied through different case scenarios of jar tests.The analysis evaluated the effect of PAC dosing in the liquid phase immediately before or after the coagulant addition.Ferric sulphate was used as the coagulant with dosages from 10 to 30 mg/L,and PAC dosages varied from 10 to 40 mg/L.The highest MIB removal efficiency (about 70%) was achieved without the coagulant addition and with the highest PAC dosage (40 mg/L).Lower MIB removal efficiencies were observed in the presence of coagulant,showing a clear interference of the iron precipitate or coagulant in the adsorption process.The degree of interference of the coagulation process in the MIB removal was proportional to the ratio of ferric hydroxide mass to the PAC mass.For both cases of PAC dosing,upstream and downstream of the coagulant injection point,the MIB removal efficiency was similar.However,MIB removal efficiency was 15% lower when compared with experiments without the coagulant application.This interference in the MIB adsorption occurs potentially because the coagulant coats the surface of the carbon and interferes with the MIB coming in contact with the carbon's surface and pores.This constraint requires an increase of the PAC dosage to provide the same efficiency observed without coagulation.

  1. Effect of BaO on Catalytic Activity of Pt-Rh TWC

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    The effects of BaO doping on the three-way catalytic activity of Pt-Rh catalyst and on water-gas shift were investigated. The results show that the light-off temperatures of hydrocarbon and carbon monoxide and nitrogen oxides of the fresh catalysts slightly differ from those of the aged catalysts, and the catalysts containing CeO2-ZrO2-BaO have lower lightoff temperature and better catalytic activity than these containing BaO and CeO2-ZrO2 after hydrothermal aging for 5 h at 1000 C. The catalysts were characterized by means of the temperature-programmed reduction (TPR) in hydrogen and the temperature-programmed desorption (TPD) in oxygen. It is confirmed that the suggested route of CeO2-ZrO2-BaO by coprecipitation can improve the catalytic activity of catalysts.

  2. mb

    African Journals Online (AJOL)

    An instructor or teacher of veterinary medicine would also find the computer useful in preparation of ..... low cost. It also provides the capability to integrate normal text with graphics and even pictures. .... would be a reality to veterinary practices.

  3. Broadband distortion modeling in Lyman-$\\alpha$ forest BAO fitting

    CERN Document Server

    Blomqvist, Michael; Bautista, Julian E; Ariño, Andreu; Busca, Nicolás G; Miralda-Escudé, Jordi; Slosar, Anže; Font-Ribera, Andreu; Margala, Daniel; Schneider, Donald P; Vazquez, Jose A

    2015-01-01

    In recent years, the Lyman-$\\alpha$ absorption observed in the spectra of high-redshift quasars has been used as a tracer of large-scale structure by means of the three-dimensional Lyman-$\\alpha$ forest auto-correlation function at redshift $z\\simeq 2.3$, but the need to fit the quasar continuum in every absorption spectrum introduces a broadband distortion that is difficult to correct and causes a systematic error for measuring any broadband properties. We describe a $k$-space model for this broadband distortion based on a multiplicative correction to the power spectrum of the transmitted flux fraction that suppresses power on scales corresponding to the typical length of a Lyman-$\\alpha$ forest spectrum. Implementing the distortion model in fits for the baryon acoustic oscillation (BAO) peak position in the Lyman-$\\alpha$ forest auto-correlation, we find that the fitting method recovers the input values of the linear bias parameter $b_{F}$ and the redshift-space distortion parameter $\\beta_{F}$ for mock dat...

  4. Take a bao if you are not superstitious.

    Science.gov (United States)

    Lim, Erle C H; Oh, Vernon M S; Quek, Amy M L; Seet, Raymond C S

    2007-03-01

    Singaporeans are superstitious, and medical staff are no exception to the rule. We conducted a survey to determine the prevalence of superstitious beliefs and practices amongst doctors, nurses and medical students in Singapore. Internet and face-to-face surveys of 68 respondents, all of whom completed the survey after being threatened with curses and hexes. Sixty-eight doctors, nurses and medical students responded to our survey. Only 11 admitted to being superstitious, yet 31 believed in the ill-fortune associated with eating bao or meat dumplings, 6 in the nefarious powers of black (5) or red (1) outfits on call, and 14 believed that bathing (6 insisting on the powers of the seven-flower bath) prior to the onset of a call portended good fortune, in terms of busy-ness of a call. Twenty-four believed in "black clouds", i.e. people who attracted bad luck whilst on call, and 32 refused to mouth the words "having a good call" until the day after the event. We discovered 2 hitherto undescribed and undiscovered superstitions, namely the benefits of eating bread and the need to avoid beef, for the good and ill fortune associated with their ingestion. Superstitious practices are alive and well in modern-day Singapore, the practice not necessarily being restricted to the poorly-educated or foolish.

  5. Progress towards BChE tethered with a reactivating ligand: a pseudo-catalytic nerve agent bioscavenger (Poster)

    NARCIS (Netherlands)

    Koning, M.C. de; Nachon, F.; Worek, F.; Brazzolotto, X.; Berthoin, L.; Trovaslet-Leroy, M.; Noort, D.; Grol, M. van; Alkema, D.; Muns, J.

    2012-01-01

    Human butyrylcholinesterase (hBChE) constitutes a promising alternative to presently available protection against organophosphate (OP) poisoning. This biological scavenger avoids the reaction of the OP with human acetylcholinesterase (hAChE) by covalent sequestration. One major drawback of the stoic

  6. Tension between SNeIa and BAO: current status and future forecasts

    Energy Technology Data Exchange (ETDEWEB)

    Escamilla-Rivera, Celia [Astrophysics, University of Oxford, DWD, Kebble Road, Oxford, OX1 3RH (United Kingdom); Lazkoz, Ruth; Salzano, Vincenzo; Sendra, Irene, E-mail: celia_escamilla@ehu.es, E-mail: ruth.lazkoz@ehu.es, E-mail: vincenzo.salzano@ehu.es, E-mail: irene.sendra@ehu.es [Fisika Teorikoaren eta Zientziaren Historia Saila, Zientzia eta Teknologia Fakultatea, Euskal Herriko Unibertsitatea, 644 Posta Kutxatila, 48080 Bilbao (Spain)

    2011-09-01

    Using real and synthetic Type Ia SNe (SNeIa) and baryon acoustic oscillations (BAO) data representing current observations forecasts, this paper investigates the tension between those probes in the dark energy equation of state (EoS) reconstruction considering the well known CPL model and Wang's low correlation reformulation. In particular, here we present simulations of BAO data from both the the radial and transverse directions. We also explore the influence of priors on Ω{sub m} and Ω{sub b} on the tension issue, by considering 1σ deviations in either one or both of them. Our results indicate that for some priors there is no tension between a single dataset (either SNeIa or BAO) and their combination (SNeIa+BAO). Our criterion to discern the existence of tension (σ-distance) is also useful to establish which is the dataset with most constraining power; in this respect SNeIa and BAO data switch roles when current and future data are considered, as forecasts predict and spectacular quality improvement on BAO data. We also find that the results on the tension are blind to the way the CPL model is addressed: there is a perfect match between the original formulation and that by the correlation optimized proposed in Wang (2008), but the errors on the parameters are much narrower in all cases of our exhaustive exploration, thus serving the purpose of stressing the convenience of this reparametrization.

  7. Matched interface and boundary (MIB) for the implementation of boundary conditions in high-order central finite differences.

    Science.gov (United States)

    Zhao, Shan; Wei, G W

    2009-03-19

    High-order central finite difference schemes encounter great difficulties in implementing complex boundary conditions. This paper introduces the matched interface and boundary (MIB) method as a novel boundary scheme to treat various general boundary conditions in arbitrarily high-order central finite difference schemes. To attain arbitrarily high order, the MIB method accurately extends the solution beyond the boundary by repeatedly enforcing only the original set of boundary conditions. The proposed approach is extensively validated via boundary value problems, initial-boundary value problems, eigenvalue problems, and high-order differential equations. Successful implementations are given to not only Dirichlet, Neumann, and Robin boundary conditions, but also more general ones, such as multiple boundary conditions in high-order differential equations and time-dependent boundary conditions in evolution equations. Detailed stability analysis of the MIB method is carried out. The MIB method is shown to be able to deliver high-order accuracy, while maintaining the same or similar stability conditions of the standard high-order central difference approximations. The application of the proposed MIB method to the boundary treatment of other non-standard high-order methods is also considered.

  8. Immunohistochemical expression of MIB-1 and PCNA in precancerous and cancerous lesions of uterine cervix

    Directory of Open Access Journals (Sweden)

    Madhu Mati Goel

    2013-01-01

    Full Text Available Background and Objective: The present study was done to analyze the immunoexpression of diagnostic markers (MIB-1: molecular immunology borstel and PCNA: proliferating cell nuclear antigen in grading cervical intraepithelial lesion (CIN and squamous cell carcinoma (SCC in cervix. Setting and Design: Total 150 cervical biopsies were divided into four groups respectively; Group I-Normal (n = 32, Group II- CIN (n = 60, Group III- SCC (n = 44, Group IV- CA cervix (n = 14 respectively. Materials and Methods: These biopsies were stained with monoclonal antibodies by streptavidin--biotin method. Mean labeling index was calculated and grading was performed using the I--III scoring system. Statistical Analysis: Findings were correlated with age and menopausal status. Statistical analysis was done by using student sample′t′ test and analysis of variance (ANOVA by SPSS 10 package. Results: MIB-1 immunostaining was positive in 112/150 (74.6% cases and PCNA in 118 /150 (78.6% cases. Labeling indices showed linear progression from normal to CIN to SCC to cancer lesion. Few cases of low-grade CIN lesion had high proliferative index. A significant positive correlation was found between age and PCNA and MIB-1 values (P < 0.05 when comparison was made for all the cases. Conclusion: These markers may be useful in identifying low-grade CIN lesion with high proliferative index. These cases should be kept for follow up studies so that proper intervention can be taken at an early stage. This method is simple and cost effective and can easily be done in formaline-fixed paraffin embedded tissues in a clinical laboratory for grading CIN and SCC lesions in cervix.

  9. Expression of Ki-67 (MIB-1) and GLUT-1 proteins in non-advanced prostatic cancer.

    Science.gov (United States)

    Luczynska, Elzbieta; Gasinska, Anna; Wilk, Waclaw

    2012-12-01

    The expression of Ki-67 (MIB-1) and glucose transporter-1 (GLUT-1) were evaluated in patients with clinically localized prostate cancer (PC) who had undergone radical prostatectomy with curative intent. 140 low advanced PC specimens were studied. Protein expression was assessed immunohistochemically on tumour sections and expressed as a labelling index, i.e. the percentage of positively stained cells. In the case of Ki-67 nuclear staining and in the case of GLUT-1 membrane and cytoplasmic staining was considered as positive. The patients' mean age was 62.9 ±6.2 years. There were 13 (9.3%) at pTNM stage 1, 78 (55.7%) at stage 2, 40 (28.6%) at stage 3 and 9 (6.4%) at stage 4, respectively. 75 (53.6%) tumours were well differentiated (Gleason score ≤6), 52 (37.1%) moderately differentiated (Gleason score of 7) and 13 (9.3%) poorly differentiated (Gleason score 8-10). The mean pre-operative serum PSA was 9.9 ± SE 0.5 ng/ml, and the mean LI was equal to 8.1 ±0.6% and 29.7 ±2.0%, for MIB-1 and GLUT-1, respectively. Increase of pathological tumor volume and tumor grade was associated with statistically significant growth of PSA (p GLUT-1 LI the relation was not significant. Ki-67 expression was correlated with PSA levels (p = 0.013) and GLUT-1 scores (p = 0.04). In PC, an increase in the proliferation rate (higher MIB-1LI) in higher pTNM stages and tumour grades may point to Ki-67 as a good marker of biological aggressiveness useful in selecting patients for more aggressive treatment. A correlation between proliferation and GLUT-1 score may be the evidence of active glycolytic metabolism in hypoxic regions.

  10. Script MIB and its implementation%Script MIB及其实现

    Institute of Scientific and Technical Information of China (English)

    李嵩泉; 刘福杰

    2004-01-01

    讨论了IETF制定的分布式网络管理中的一个重要标准一Script MIB及其实现方法.第一部分讲述了它的产生背景和主要用途,与移动代理技术之间的关系;接着介绍了它的功能和六个关键组成表;最后设计了一个用于实际的Script MIB Agent的实现方法.

  11. TINGKAT KEGANASAN KANKER SERVIKS PASIEN PRA-RADIASI MELALUI PEMERIKSAAN AgNORs, MIB-1 DAN Cas- 3

    Directory of Open Access Journals (Sweden)

    Iin Kurnia

    2012-09-01

    Full Text Available Kanker serviks sering ditemukan di negara berkembang. Pengobatan kanker melalui radioterapi untuk mengetahui tingkat proliferasi dan mengurangi tingkat keganasan. Biomarker proliferasi dan apoptosis berupa AgNORs, MIB-1, dan Caspase 3. Namun belum dijelaskan mengenai korelasi ketiga biomarker dalam kaitannya dengan proliferasi dan apoptosis pada sel kanker serviks. Tujuan penelitian untuk mengetahui korelasi antara AgNORs, MIB-1, dan apoptosis pada kanker serviks. Penelitian observasional laboratoris menggunakan metode pewarnaan dengan menekankan kontras warna antara sitoplasma dan inti sel. Objek berupa sediaan mikroskopis dari 30 biopsi pasien kanker serviks. Pengambilan data dengan metode crocker dan blind manner. Analisis data menggunakan uji korelasi, dari ju mlah 21 pasien yang diamati menunjukkan. AgNORs dan MIB-1 memiliki angka relatif tinggi. Angka yang diperoleh ini berbanding terbalik dengan apoptosis yang relatif rendah. Korelasi antara AgNORs dengan MIB-1 menunjukkan r= 0,33 dan p= 0,15. AgNORs dengan apoptosis memiliki korelasi negatif yakni, r=-0,08 dan p= 0,73. MIB-1 dengan apoptosis memiliki korelasi negatif pula r= -0,18 dan p= 0,43. Kesimpulannya korelasi AgNORs dengan apoptosis memiliki kecenderungan lebih baik dari pada MIB-1 dengan apoptosis.Cervical cancer is often found in the developing countries. The treatment of cancer through radiotherapy was performed to determine the proliferation level and to reduce the malignancy level of cancer. The proliferation and apoptotic biomarkers were AgNORs, MIB-1, and Cas- 3. However, the correlation between the three biomarkers in relation to the proliferation and apoptosis in cervical cancer cells was not clear. The purpose of the study was to determine the correlation between AgNORs, MIB-1 and apoptosis in cervical cancer. This study was an observational research laboratory using a staining method to emphasize the color contrast between the cytoplasm and the nucleus of the cells. The

  12. Towards MIB-1 and p53 detection in glioma magnetic resonance image: a novel computational image analysis method

    Science.gov (United States)

    Liu, Chenbin; Zhang, Haishi; Pan, Ying; Huang, Fengping; Xia, Shunren

    2012-12-01

    Glioma is the primary tumor in the central nervous system, and poses one of the greatest challenges in clinical treatment. MIB-1 and p53 are the most useful biomarkers for gliomas and could help neurosurgeons establish a therapeutic schedule. However, these biomarkers are commonly detected with the help of immunohistochemistry (IHC), which wastes time and energy and is often influenced by subjective factors. To reduce the subjective factors and improve the efficiency in the judgment of IHC, a novel magnetic resonance image (MRI) analysis method is proposed in the present study to detect the expression status of MIB-1 and p53 in IHC. The proposed method includes two kinds of MRI acquisition (FLAIR and T1 FLAIR images), regions of interest (ROIs) selection, texture features (i.e. the gray level gradient co-occurrence matrix (GLGCM), Minkowski functions (MFs), etc) extraction in ROIs, and classification with a support vector machine in a leave-one-out cross validation strategy. By classifying the ROIs, the performance of the method was evaluated by accuracy, area under ROC curve (AUC), etc. A high accuracy (0.7640 ± 0.0225) and AUC (0.7873 ± 0.0377) for MIB-I detection were achieved. In terms of the texture features, 0.7621 ± 0.0199, 0.7666 ± 0.0365 and 0.7426 ± 0.0451 AUC can be obtained using only GLCM, RLM or GLGCM for MIB-1 detection, respectively. In all, the experimental results demonstrated that MR image texture features are associated with the expression status of MIB-1 and p53. The proposed method has the potential to realize high accuracy and robust detection for MIB-I expression status, which makes it promising for clinical glioma diagnosis and prognosis.

  13. Dal CERN, flusso si dati a una media di 600 megabytes al secondo per dieci giorni consecutivi

    CERN Multimedia

    2005-01-01

    The supercomputer Grid took up successfully its first technologic challenge. Egiht supercomputing centers have supported on internet a continuous flow of data from CERN in Geneva and directed them to seven centers in Europe and United States

  14. Rapid 500-mb cyclogenesis and anticyclogenesis

    Science.gov (United States)

    Alberta, Timothy L.; Colucci, Stephen J.; Davenport, J. C.

    1991-01-01

    The process of intensification of 500-mb cyclones and anticyclones as represented in NMC's 500-mb analyses is investigated, using a sample of records for 500-mb cyclones and anticyclones drawn from the cool season of September 1983 through May 1984 to determine the statistically large intensification rates and thus to establish criteria for rapid intensification. From the catalog of all rapidly intensifying 500-mb cyclones and anticyclones defined by these criteria, seasonal and geographical frequency distributions of cyclogenesis and anticyclogenesis is constructed. It is shown that, for the data sample used in this analysis, the operational forecast models tend to underpredict rapid developments of 500-mb cyclones and anticyclones.

  15. Cálculo de la distancia aparente de códigos abelianos : códigos BCH multivariables

    OpenAIRE

    Bueno Carreño, Diana Haidive

    2014-01-01

    El objetivo principal de este trabajo es el estudio del cálculo de la distancia aparente de un código abeliano, la cual es una cota para su distancia mínima. Asimismo, también son objeto de este trabajo el desarrollo de la noción de cota BCH y código BCH multivariable, de las construcciones multivariables y las aplicaciones a los códigos cíclicos que se desprenden de dichas nociones. Un primer problema concreto que abordamos en esta tesis es determinar cuándo en un código cíclico la ma...

  16. BigBOSS: The Ground-Based Stage IV BAO Experiment

    Energy Technology Data Exchange (ETDEWEB)

    Schlegel, David; Bebek, Chris; Heetderks, Henry; Ho, Shirley; Lampton, Michael; Levi, Michael; Mostek, Nick; Padmanabhan, Nikhil; Perlmutter, Saul; Roe, Natalie; Sholl, Michael; Smoot, George; White, Martin; Dey, Arjun; Abraham, Tony; Jannuzi, Buell; Joyce, Dick; Liang, Ming; Merrill, Mike; Olsen, Knut; Salim, Samir

    2009-04-01

    The BigBOSS experiment is a proposed DOE-NSF Stage IV ground-based dark energy experiment to study baryon acoustic oscillations (BAO) and the growth of structure with an all-sky galaxy redshift survey. The project is designed to unlock the mystery of dark energy using existing ground-based facilities operated by NOAO. A new 4000-fiber R=5000 spectrograph covering a 3-degree diameter field will measure BAO and redshift space distortions in the distribution of galaxies and hydrogen gas spanning redshifts from 0.2< z< 3.5. The Dark Energy Task Force figure of merit (DETF FoM) for this experiment is expected to be equal to that of a JDEM mission for BAO with the lower risk and cost typical of a ground-based experiment.

  17. Errores sistemáticos en la escala de las BAO

    Science.gov (United States)

    Scóccola, C. G.

    2016-08-01

    The large current and future galaxy surveys require very precise theoretical predictions of the baryon acoustic oscillations (BAO). In this contribution article I briefly comment on the physical processes that lead to these oscillations, and review a work where we use large -body cosmological simulations to study any systematic shift and damping of the BAO due to non-linear effects in the growth of structure. The precision of the simulations used for this work allows to resolve the dark matter subhalos, which enable to study with great accuracy (better than 0.02 for dark matter and 0.07 for low bias halos) small shifts toward larger wavenumbers (), and a non-linear damping of the BAO in the power spectrum.

  18. Dimensioning BCH codes for coherent DQPSK systems with laser phase noise and cycle slips

    DEFF Research Database (Denmark)

    Leong, Miu Yoong; Larsen, Knud J.; Jacobsen, Gunnar

    2014-01-01

    Forward error correction (FEC) plays a vital role in coherent optical systems employing multi-level modulation. However, much of coding theory assumes that additive white Gaussian noise (AWGN) is dominant, whereas coherent optical systems have significant phase noise (PN) in addition to AWGN. Thi...... approach for a target post-FEC BER of 10-5. Codes dimensioned with our bivariate binomial model meet the target within 0.2-dB signal-to-noise ratio....... these statistics to parameterize a bivariate binomial model that describes the distribution of bit errors. In this way, we relate pre-FEC statistics to post-FEC BER and BCH codes. Our method is applicable to pre-FEC BER around 10-3 and any post-FEC BER. Using numerical simulations, we evaluate the accuracy of our......Forward error correction (FEC) plays a vital role in coherent optical systems employing multi-level modulation. However, much of coding theory assumes that additive white Gaussian noise (AWGN) is dominant, whereas coherent optical systems have significant phase noise (PN) in addition to AWGN...

  19. BCH Codes for Coherent Star DQAM Systems with Laser Phase Noise

    Science.gov (United States)

    Leong, Miu Yoong; Larsen, Knud J.; Jacobsen, Gunnar; Zibar, Darko; Sergeyev, Sergey; Popov, Sergei

    2017-03-01

    Coherent optical systems have relatively high laser phase noise, which affects the performance of forward error correction (FEC) codes. In this paper, we propose a method for selecting Bose-Chaudhuri-Hocquenghem (BCH) codes for coherent systems with star-shaped constellations and M-ary differential quadrature amplitude modulation (DQAM). Our method supports constellations of any order M which is a power of 2, and includes differential M-ary phase shift keying as a special case. Our approach is straightforward, requiring only short pre-FEC simulations to parameterize a statistical model, based on which we select codes analytically. It is applicable to pre-FEC bit error rates (BERs) of around 10-3. We evaluate the accuracy of our approach using numerical simulations. For a target post-FEC BER of 10-5, codes selected with our method yield BERs within 2× target. Lastly, we extend our method to systems with interleaving, which enables us to use codes with lower overhead.

  20. Influence of BaO in perovskite electrodes for the electrochemical reduction of NOx

    DEFF Research Database (Denmark)

    Simonsen, Vibe Louise Ernlund; Johnsen, M.M.; Kammer Hansen, Kent

    2007-01-01

    Using the point electrode method, the effect of BaO on electrochemical reduction of NO (x) was investigated using the perovskites La0.85Sr0.15MnO3 (LSM15) and La0.85Sr0.15CoO3 (LSCo15) as electrode materials. The experiments were carried out in the temperature range 400-600 degrees C in 1% NO and...... favored oxygen reduction compared to reduction of nitric oxide. The LSCO15 electrode containing BaO reacted to form a K2NiF4-structure and was not tested further....

  1. The fundamental lemma and the Hitchin fibration [after Ngo Bao Chau

    CERN Document Server

    Hales, Thomas C

    2011-01-01

    This article is a Bourbaki seminar report on Ngo Bao Chau's proof of the fundamental lemma. About thirty years ago, R. P. Langlands conjectured a collection of identities to hold among integrals over conjugacy classes in reductive groups. Ngo Bao Chau has proved these identities (collectively called the fundamental lemma) by interpreting the integrals in terms of the cohomology of the fibers of the Hitchin fibration. The fundamental lemma has profound consequences for the theory of automorphic representations. Significant recent theorems in number theory use the fundamental lemma as an ingredient in their proofs.

  2. Quantitative method to determine the regional drinking water odorant regulation goals based on odor sensitivity distribution: illustrated using 2-MIB.

    Science.gov (United States)

    Yu, Jianwei; An, Wei; Cao, Nan; Yang, Min; Gu, Junong; Zhang, Dong; Lu, Ning

    2014-07-01

    Taste and odor (T/O) in drinking water often cause consumer complaints and are thus regulated in many countries. However, people in different regions may exhibit different sensitivities toward T/O. This study proposed a method to determine the regional drinking water odorant regulation goals (ORGs) based on the odor sensitivity distribution of the local population. The distribution of odor sensitivity to 2-methylisoborneol (2-MIB) by the local population in Beijing, China was revealed by using a normal distribution function/model to describe the odor complaint response to a 2-MIB episode in 2005, and a 2-MIB concentration of 12.9 ng/L and FPA (flavor profile analysis) intensity of 2.5 was found to be the critical point to cause odor complaints. Thus the Beijing ORG for 2-MIB was determined to be 12.9 ng/L. Based on the assumption that the local FPA panel can represent the local population in terms of sensitivity to odor, and that the critical FPA intensity causing odor complaints was 2.5, this study tried to determine the ORGs for seven other cities of China by performing FPA tests using an FPA panel from the corresponding city. ORG values between 12.9 and 31.6 ng/L were determined, showing that a unified ORG may not be suitable for drinking water odor regulations. This study presents a novel approach for setting drinking water odor regulations.

  3. Modeling nonequilibrium adsorption of MIB and sulfamethoxazole by powdered activated carbon and the role of dissolved organic matter competition.

    Science.gov (United States)

    Shimabuku, Kyle K; Cho, Hyukjin; Townsend, Eli B; Rosario-Ortiz, Fernando L; Summers, R Scott

    2014-12-02

    This study demonstrates that the ideal adsorbed solution theory-equivalent background compound (IAST-EBC) as a stand-alone model can simulate and predict the powdered activated carbon (PAC) adsorption of organic micropollutants found in drinking water sources in the presence of background dissolved organic matter (DOM) under nonequilibrium conditions. The IAST-EBC represents the DOM competitive effect as an equivalent background compound (EBC). When adsorbing 2-methylisoborneol (MIB) with PAC, the EBC initial concentration was a similar percentage, on average 0.51%, of the dissolved organic carbon in eight nonwastewater impacted surface waters. Using this average percentage in the IAST-EBC model yielded good predictions for MIB removal in two nonwastewater impacted waters. The percentage of competitive DOM was significantly greater in wastewater impacted surface waters, and varied markedly in DOM size fractions. Fluorescence parameters exhibited a strong correlation with the percentage of competitive DOM in these waters. Utilizing such correlations in the IAST-EBC successfully modeled MIB and sulfamethoxazole adsorption by three different PACs in the presence of DOM that varied in competitive effect. The influence of simultaneous coagulant addition on PAC adsorption of micropollutants was also investigated. Coagulation caused the DOM competitive effect to increase and decrease with MIB and sulfamethoxazole, respectively.

  4. Prediction of powdered activated carbon doses for 2-MIB removal in drinking water treatment using a simplified HSDM approach.

    Science.gov (United States)

    Yu, Jianwei; Yang, Fong-Chen; Hung, Wei-Nung; Liu, Chia-Ling; Yang, Min; Lin, Tsair-Fuh

    2016-08-01

    The addition of powdered activated carbon (PAC) is an effective measure to cope with seasonal taste and odor (T&O) problems caused by 2-methylisoborneol (2-MIB) and trans-1, 10-dimethyl-trans-9-decalol (geosmin) in drinking water. Some T&O problems are episodic in nature, and generally require rapid responses. This paper proposed a simplified approach for the application of the homogenous surface diffusion model (HSDM) to predict the appropriate PAC doses for the removal of 2-MIB. Equilibrium and kinetic experiments were performed for 2-MIB adsorption onto five PACs in three source waters. The simplified HSDM approach was compared with the experimental data, by assigning the Freundlich 1/n value in the range of 0.1-1.0 and obtaining the Freundlich equilibrium parameter K value through a 6-hr adsorption kinetic test. The model describes the kinetic adsorption data very well for all of the tested PACs in different source waters. The results were validated using the data obtained from one full scale water treatment plant, and the differences between the predicted and observed results were within 10% range. This simplified HSDM approach may be applied for the rapid determination of PAC doses for water treatment plants when faced with 2-MIB episodes in source waters.

  5. Application of SimpleTester in MIB Test%SimpleTester在MIB测试中的应用

    Institute of Scientific and Technical Information of China (English)

    曾定洲; 唐丽莉

    2013-01-01

      随着大家对网络设备易用性要求的提高,人们越来越倾向于完全使用图形化界面的网管系统来完成对设备的管理和维护。而MIB(Management Information Base)作为网管系统管理网络设备的“中间件”,其定义、设计的规范性和标准遵从性也变得越来越重要。传统的MIB测试工具效率低下,文中介绍了一种高效测试工具SimpleTester在MIB测试中的应用,详细介绍了SimpleTester的框架结构以及使用SimpleTester进行MIB测试的一般流程,并通过例子说明了SimpleTest-er能帮忙我们极大地提高测试效率和质量。%With the improved easy-using requirements of network equipment,there is more and more inclined to use graphical network management system to complete the management and maintenance of network element. MIB (Management Information Base) as the "middleware" between network management system and network element,its definition,design specifications and standards compliance is also becoming increasingly important. As the inefficiency of traditional MIB testing tools,introduce an efficient testing tool SimpleTester in MIB test,in detail the framework structure of SimpleTester and general process of how to use SimpleTester in MIB test. In the end,an example is given to illustrate greatly improve the test efficiency and quality with SimpleTester's help.

  6. Odours from pulp mill effluent treatment ponds: the origin of significant levels of geosmin and 2-methylisoborneol (MIB).

    Science.gov (United States)

    Watson, Susan B; Ridal, Jeff; Zaitlin, Beryl; Lo, Amy

    2003-06-01

    Pulp and paper mills are well known for their sharp, sulphurous stack emissions, but the secondary treatment units also can be significant contributors to local odour. This study investigated the source(s) of earthy/musty emissions from a mixed hardwood pulp mill in response to a high local odour. Samples from five sites in the mill over five months were analyzed for earthy/musty volatile organic compounds (VOCs), examined microscopically, and plated for bacteria and moulds. In all cases, activated sludge showed substantial geosmin levels and to a lesser extent 2-methylisoborneol (MIB) at 2000-9000 times their odour threshold concentrations (OTCs). These VOCs were lower or absent upstream and downstream, suggesting that they were produced within the bioreactor. Geosmin and MIB were highest in late summer and declined over winter, and correlated with different operating parameters. Geosmin was most closely coupled with temperature and MIB with nitrogen uptake. Cyanobacteria were present in all sludge samples, but actinomycetes were not found. Gram-negative bacteria and one fungal species isolated from the bioreactor and secondary outfall tested negative for geosmin or MIB. We conclude: (i) geosmin and MIB contribute significantly to airborne odours from this mill, but are diluted below OTC levels at the river; (ii) these VOCs are generated by biota in the activated sludge; and (iii) cyanobacteria are likely primary source(s). The growth of cyanobacteria in activated sludge represents a loss of energy to the heterotrophic population; thus earthy/musty odours may represent a diagnostic for less than optimal conditions.

  7. Normalization of periodontal tissues in osteopetrotic mib mutant rats, treated with CSF-1

    Science.gov (United States)

    Wojtowicz, A.; Yamauchi, M.; Sotowski, R.; Ostrowski, K.

    1998-01-01

    The osteopetrotic mib mutation in rats causes defects in the skeletal bone tissue in young animals. These defects, i.e. slow bone remodelling, changes in both crystallinity and mineral content, are transient and undergo normalization, even without any treatment in 6-wk-old animals. Treatment with CSF-1 (colony stimulating factor-1) accelerates the normalization process in skeletal bones. The periodontal tissues around the apices of incisors show abnormalities caused by the slow remodelling process of the mandible bone tissue, the deficiency of osteoclasts and their abnormal morphology, as well as the disorganization of periodontal ligament fibres. In contrast to the skeletal tissues, these abnormalities would not undergo spontaneous normalization. Under treatment with colony stimulating factor 1 (CSF-1), the primitive bone trabeculae of mandible are resorbed and the normalization of the number of osteoclasts and their cytology occurs. The organization of the periodontal ligament fibres is partially restored, resembling the histological structure of the normal one.

  8. Measurement of Ki-67 antigen in 159 pituitary adenomas using the MIB-1 monoclonal antibody

    Directory of Open Access Journals (Sweden)

    C.B. Pizarro

    2004-02-01

    Full Text Available Pituitary adenomas sometimes show rapid growth and recurrence, and about one third invade the structures surrounding the sella turcica. In an attempt to determine aggressive behavior at an early stage, we used the MIB-1 antibody to identify the Ki-67 antigen. The present study was designed to evaluate pituitary adenomatous tissue in terms of secretion and proliferation and to correlate the Ki-67 index with hormone phenotype and invasive behavior. Material from 159 patients submitted to one or more resections of pituitary adenomas was evaluated. Forty-two non-secretory adenomas and 43 adenomas immunoreactive for growth hormone, 19 for prolactin, 18 for growth hormone and prolactin, 16 for adrenocorticotropic hormone (ACTH, and 21 cases of plurihormonal/gonadotropin adenomas were detected by immunohistochemistry. The MIB-1 antibody was positive in 139 samples and the Ki-67 index ranged from 0.16 to 15.48% (mean = 1.22 ± 2.09%, with no significant difference between genders, age groups, or secretory and non-secretory status. The Ki-67 index was higher in ACTH-secreting adenomas. Invasive pituitary adenomas had a significantly higher Ki-67 index (2.01 ± 3.15% than macroadenomas with or without supra-sellar extension (1.12 ± 1.87%; P = 0.02. The index was not significantly different in the subgroup of adenomas with invasion of the cavernous sinus compared to groups with other types of invasion. We conclude that tumoral proliferative activity evaluated by the detection of the Ki-67 antigen is significantly higher in invasive than noninvasive adenomas, information which can be useful in therapeutic postoperative management since index cut-off values associated with aggressive behavior can be established.

  9. Density-dependent clustering: I. Pulling back the curtains on motions of the BAO peak

    CERN Document Server

    Neyrinck, Mark C; McCullagh, Nuala; Szalay, Alex; Falck, Bridget; Wang, Jie

    2016-01-01

    The most common statistic used to analyze large-scale structure surveys is the correlation function, or power spectrum. Here, we show how `slicing' the correlation function on local density brings sensitivity to interesting non-Gaussian features in the large-scale structure, such as the expansion or contraction of baryon acoustic oscillations (BAO) according to the local density. The sliced correlation function measures the large-scale flows that smear out the BAO, instead of just correcting them as reconstruction algorithms do. Thus, we expect the sliced correlation function to be useful in constraining the growth factor, and modified gravity theories that involve the local density. We find that the full run of the BAO peak location with density is best revealed when slicing on a $\\sim$40 Mpc/$h$ filtered density. But slicing on a $\\sim100$ Mpc/$h$ filtered density may be most useful in distinguishing between underdense and overdense regions, whose BAO peaks are shifted by a substantial $\\sim$5 Mpc/$h$ at $z...

  10. BioAssay Ontology (BAO: a semantic description of bioassays and high-throughput screening results

    Directory of Open Access Journals (Sweden)

    Smith Robin P

    2011-06-01

    Full Text Available Abstract Background High-throughput screening (HTS is one of the main strategies to identify novel entry points for the development of small molecule chemical probes and drugs and is now commonly accessible to public sector research. Large amounts of data generated in HTS campaigns are submitted to public repositories such as PubChem, which is growing at an exponential rate. The diversity and quantity of available HTS assays and screening results pose enormous challenges to organizing, standardizing, integrating, and analyzing the datasets and thus to maximize the scientific and ultimately the public health impact of the huge investments made to implement public sector HTS capabilities. Novel approaches to organize, standardize and access HTS data are required to address these challenges. Results We developed the first ontology to describe HTS experiments and screening results using expressive description logic. The BioAssay Ontology (BAO serves as a foundation for the standardization of HTS assays and data and as a semantic knowledge model. In this paper we show important examples of formalizing HTS domain knowledge and we point out the advantages of this approach. The ontology is available online at the NCBO bioportal http://bioportal.bioontology.org/ontologies/44531. Conclusions After a large manual curation effort, we loaded BAO-mapped data triples into a RDF database store and used a reasoner in several case studies to demonstrate the benefits of formalized domain knowledge representation in BAO. The examples illustrate semantic querying capabilities where BAO enables the retrieval of inferred search results that are relevant to a given query, but are not explicitly defined. BAO thus opens new functionality for annotating, querying, and analyzing HTS datasets and the potential for discovering new knowledge by means of inference.

  11. Co2FeAl based magnetic tunnel junctions with BaO and MgO/BaO barriers

    Directory of Open Access Journals (Sweden)

    J. Rogge

    2015-07-01

    Full Text Available We succeed to integrate BaO as a tunneling barrier into Co2FeAl based magnetic tunnel junctions (MTJs. By means of Auger electron spectroscopy it could be proven that the applied annealing temperatures during BaO deposition and afterwards do not cause any diffusion of Ba neither into the lower Heusler compound lead nor into the upper Fe counter electrode. Nevertheless, a negative tunnel magnetoresistance (TMR ratio of -10% is found for Co2FeAl (24 nm / BaO (5 nm / Fe (7 nm MTJs, which can be attributed to the preparation procedure and can be explained by the formation of Co- and Fe-oxides at the interfaces between the Heusler and the crystalline BaO barrier by comparing with theory. Although an amorphous structure of the BaO barrier seems to be confirmed by high-resolution transmission electron microscopy (TEM, it cannot entirely be ruled out that this is an artifact of TEM sample preparation due to the sensitivity of BaO to moisture. By replacing the BaO tunneling barrier with an MgO/BaO double layer barrier, the electric stability could effectively be increased by a factor of five. The resulting TMR effect is found to be about +20% at room temperature, although a fully antiparallel state has not been realized.

  12. Self-Similar Bumps and Wiggles: Isolating the Evolution of the BAO Peak with Power-law Initial Conditions

    CERN Document Server

    Orban, Chris

    2011-01-01

    Motivated by cosmological surveys that demand accurate theoretical modeling of the baryon acoustic oscillation (BAO) feature in galaxy clustering, we analyze N-body simulations in which a BAO-like gaussian bump modulates the linear theory correlation function \\xi_L(r)=(r_0/r)^{n+3} of an underlying self-similar model with initial power spectrum P(k)=A k^n. These simulations test physical and analytic descriptions of BAO evolution far beyond the range of most studies, since we consider a range of underlying power spectra (n=-0.5, -1, -1.5) and evolve simulations to large effective correlation amplitudes (equivalent to \\sigma_8=4-12 for r_bao = 100 Mpc/h). In all cases, non-linear evolution flattens and broadens the BAO bump in \\xi(r) while approximately preserving its area. This evolution resembles a "diffusion" process in which the bump width \\sigma_bao is the quadrature sum of the linear theory width and a length proportional to the rms relative displacement \\Sigma_pair(r_bao}) of particle pairs separated by...

  13. Relative changes of MIB1 and PC10 labeling indexes in cell kinetics during radiotherapy for cervical squamous cell carcinoma

    Energy Technology Data Exchange (ETDEWEB)

    Oka, Kuniyuki [Dept. of Pathology, Mito Saiseikai General Hospital, Ibaraki (Japan)]|[National Inst. of Radiological Sciences, Chiba (Japan). Unit of Clinical Examinations]|[National Inst. of Radiological Sciences, Chiba (Japan). Div. of Radiation Medicine; Nakano, Takashi [National Inst. of Radiological Sciences, Chiba (Japan). Unit of Clinical Oncology]|[National Inst. of Radiological Sciences, Chiba (Japan). Div. of Radiation Medicine; Hoshi, Tanji [Tokyo Metropolitan Univ. (Japan). Inst. for Urban Studies]|[National Inst. of Radiological Siences, Chiba (Japan). Research Center of Charged Particle Therapy

    1997-12-31

    To assess the changes in cell kinetics during radiotherapy (RT) in terms of the MIB1 and PC10 labeling indexes of cancer cells, a total of 230 biopsy specimens taken from 76 consecutive patients with cervical squamous cell carcinoma treated with RT was investigated. The MIB1 and PC10 indexes represent the growth fraction and proliferative activity, respectively. The MIB1 index ranged from 11 to 67% (mean value: 38%) before RT, from 21 to 86% (50%) at 5.4 Gy, from 15 to 85% (53%) at 9 Gy, and from 0 to 69% (26%) at 27 Gy. The PC10 index ranged from 4 to 46% (23%) before RT, from 5 to 62% (26%) at 5.4 Gy, from 6 to 63% (23%) at 9 Gy, and from 0 to 35% (11%) at 27 Gy. The correlational relationships between the increment of both indexes during RT ({Delta} index) and the index before RT were analyzed on an individual basis. The {Delta} index was calculated by the index at various doses during RT minus the index before RT. Negative linear relationships were observed between the {Delta} MIB1 index at 9 Gy or {Delta} MIB1 index at 27 Gy and the MIB1 before RT, and also between the {Delta} PC10 index at 9 Gy or {Delta} PC10 index at 27 Gy and the PC10 index before RT. The linear regression coefficient values showed more than 0.5 (0.511 to 0.682) with statistical significance. According to these results, it was suggested that the cervical cancers of lower proliferative activity showed greater increases in growth fraction and proliferative activity by irradiation than those of higher proliferative tumors. These findings suggest that reversal patterns of changes in cell kinetics including growth fraction and proliferative activity took place in the early period of RT for cervical squamous cell carcinomas. The study of changes in cell cycle kinetics in human tumors during RT may have potential impact on the further development of RT. (orig.).

  14. Biodegradation of atrazine by Rhodococcus sp. BCH2 to N-isopropylammelide with subsequent assessment of toxicity of biodegraded metabolites.

    Science.gov (United States)

    Kolekar, Parag D; Phugare, Swapnil S; Jadhav, Jyoti P

    2014-02-01

    Atrazine is a persistent organic pollutant in the environment which affects not only terrestrial and aquatic biota but also human health. Since its removal from the environment is needed, atrazine biodegradation is achieved in the present study using the bacterium Rhodococcus sp. BCH2 isolated from soil, long-term treated with atrazine. The bacterium was capable of degrading about 75 % atrazine in liquid medium having pH 7 under aerobic and dark condition within 7 days. The degradation ability of the bacterium at various temperatures (20-60 °C), pH (range 3-11), carbon (glucose, fructose, sucrose, starch, lactose, and maltose), and nitrogen (ammonium molybdate, sodium nitrate, potassium nitrate, and urea) sources were studied for triumph optimum atrazine degradation. The results indicate that atrazine degradation at higher concentrations (100 ppm) was pH and temperature dependent. However, glucose and potassium nitrate were optimum carbon and nitrogen source, respectively. Atrazine biodegradation analysis was carried out by using high-performance thin-layer chromatography (HPTLC), Fourier transform infrared spectroscopy (FTIR), and liquid chromatography quadrupole time-of-flight (LC/Q-TOF-MS) techniques. LC/Q-TOF-MS analysis revealed formation of various intermediate metabolites including hydroxyatrazine, N-isopropylammelide, deisopropylhydroxyatrazine, deethylatrazine, deisopropylatrazine, and deisopropyldeethylatrazine which was helpful to propose biochemical degradation pathway of atrazine. Furthermore, the toxicological studies of atrazine and its biodegraded metabolites were executed on earthworm Eisenia foetida as a model organism with respect to enzymatic (SOD and Catalase) antioxidant defense mechanism and lipid peroxidation studies. These results suggest innocuous degradation of atrazine by Rhodococcus sp. BCH2 in nontoxic form. Therefore the Rhodococcus sp.BCH2 could prove a valuable source for the eco-friendly biodegradation of atrazine pesticide.

  15. Cross-species analyses identify the BNIP-2 and Cdc42GAP homology (BCH domain as a distinct functional subclass of the CRAL_TRIO/Sec14 superfamily.

    Directory of Open Access Journals (Sweden)

    Anjali Bansal Gupta

    Full Text Available The CRAL_TRIO protein domain, which is unique to the Sec14 protein superfamily, binds to a diverse set of small lipophilic ligands. Similar domains are found in a range of different proteins including neurofibromatosis type-1, a Ras GTPase-activating Protein (RasGAP and Rho guanine nucleotide exchange factors (RhoGEFs. Proteins containing this structural protein domain exhibit a low sequence similarity and ligand specificity while maintaining an overall characteristic three-dimensional structure. We have previously demonstrated that the BNIP-2 and Cdc42GAP Homology (BCH protein domain, which shares a low sequence homology with the CRAL_TRIO domain, can serve as a regulatory scaffold that binds to Rho, RhoGEFs and RhoGAPs to control various cell signalling processes. In this work, we investigate 175 BCH domain-containing proteins from a wide range of different organisms. A phylogenetic analysis with ~100 CRAL_TRIO and similar domains from eight representative species indicates a clear distinction of BCH-containing proteins as a novel subclass within the CRAL_TRIO/Sec14 superfamily. BCH-containing proteins contain a hallmark sequence motif R(R/Kh(R/K(R/KNL(R/KxhhhhHPs ('h' is large and hydrophobic residue and 's' is small and weekly polar residue and can be further subdivided into three unique subtypes associated with BNIP-2-N, macro- and RhoGAP-type protein domains. A previously unknown group of genes encoding 'BCH-only' domains is also identified in plants and arthropod species. Based on an analysis of their gene-structure and their protein domain context we hypothesize that BCH domain-containing genes evolved through gene duplication, intron insertions and domain swapping events. Furthermore, we explore the point of divergence between BCH and CRAL-TRIO proteins in relation to their ability to bind small GTPases, GAPs and GEFs and lipid ligands. Our study suggests a need for a more extensive analysis of previously uncharacterized BCH, 'BCH

  16. In Situ Investigation of the Mechanism of BaO Dissolution in Chloride Melts by Raman Spectroscopy

    Science.gov (United States)

    Zakir‧yanova, I. D.; Nikolaeva, E. V.; Bove, A. L.

    2015-01-01

    The mechanism of BaO dissolution in a melt of the BaCl2-KCl-NaCl eutectic mixture was determined in situ using Raman spectroscopy. The types, structures, and compositions of oxychloride groups present in the melt were established. It was found that BaO dissolution proceeded according to the reaction BaO (s) + [BaCl6]4- (soln.) → [Ba2OCl6]4- (soln.) and was accompanied by the appearance of [Ba2OCl6]4- groups (D3d symmetry) of a mixed oxychloride composition.

  17. Prognostic value of tissue protein expression levels of MIB-1 (Ki-67) in Danish ovarian cancer patients. From the 'MALOVA' ovarian cancer study

    DEFF Research Database (Denmark)

    Heeran, Mel C; Høgdall, Claus K; Kjaer, Susanne K

    2013-01-01

    The primary objective of this study was to assess the expression of MIB-1 (Ki-67) in tumour tissues from 808 patients with epithelial ovarian tumours. The second was to evaluate, whether MIB-1 (Ki-67) tissue expression levels correlate with clinicopathological parameters and prognosis...... of the disease. Using tissue arrays (TA), we analysed the MIB-1 (Ki-67) expression levels in tissues from 202 women with borderline ovarian tumours (BOT) (177 stage I, 5 stage II, 19 stage III, 1 stage IV) and 606 ovarian cancer (OC) patients (177 stage I, 64 stage II, 311 stage III, 54 stage IV). Using a 10......% cut-off level for MIB-1 (Ki-67) overexpression, 12% of the BOTs and 51% of the OCs were positive for MIB-1 (Ki-67) expression. The frequency of MIB-1 (Ki-67) expression-positive OC increased with increasing FIGO stage (p = 0.003), increasing histological grade (p ≤ 0.0001), and a significantly...

  18. Epb41l5 competes with Delta as a substrate for Mib1 to coordinate specification and differentiation of neurons.

    Science.gov (United States)

    Matsuda, Miho; Rand, Kinneret; Palardy, Greg; Shimizu, Nobuyuki; Ikeda, Hiromi; Dalle Nogare, Damian; Itoh, Motoyuki; Chitnis, Ajay B

    2016-09-01

    We identified Erythrocyte membrane protein band 4.1-like 5 (Epb41l5) as a substrate for the E3 ubiquitin ligase Mind bomb 1 (Mib1), which is essential for activation of Notch signaling. Although loss of Epb41l5 does not significantly alter the pattern of neural progenitor cells (NPCs) specified as neurons at the neural plate stage, it delays their delamination and differentiation after neurulation when NPCs normally acquire organized apical junctional complexes (AJCs) in the zebrafish hindbrain. Delays in differentiation are reduced by knocking down N-cadherin, a manipulation expected to help destabilize adherens junctions (AJs). This suggested that delays in neuronal differentiation in epb41l5-deficient embryos are related to a previously described role for Epb41l5 in facilitating disassembly of cadherin-dependent AJCs. Mib1 ubiquitylates Epb41l5 to promote its degradation. DeltaD can compete with Epb41l5 to reduce Mib1-dependent Epb41l5 degradation. In this context, increasing the number of NPCs specified to become neurons, i.e. cells expressing high levels of DeltaD, stabilizes Epb41l5 in the embryo. Together, these observations suggest that relatively high levels of Delta stabilize Epb41l5 in NPCs specified as neurons. This, we suggest, helps coordinate NPC specification with Epb41l5-dependent delamination and differentiation as neurons.

  19. Effect of oxidant exposure on the release of intracellular microcystin, MIB, and geosmin from three cyanobacteria species.

    Science.gov (United States)

    Wert, Eric C; Korak, Julie A; Trenholm, Rebecca A; Rosario-Ortiz, Fernando L

    2014-04-01

    The release of intracellular microcystin-LR (MC-LR), 2-methylisoborneol (MIB), and geosmin was investigated after the oxidation of three cyanobacteria (Microcystis aeruginosa (MA), Oscillatoria sp. (OSC), and Lyngbya sp. (LYN)). During the oxidation of 200,000 cells/mL of MA, release of intracellular MC-LR exceeded the World Health Organization (WHO) guideline of 1 μg/L during the lowest oxidant exposures (CT) tested: ozone (0 mg-min/L, below the ozone demand), chlorine (geosmin exceeded reported threshold odor values after exposure to chlorine, chlorine dioxide, and chloramine, which have low reactivity with these taste and odor compounds. Ozone oxidation of OSC yielded an increase in MIB concentration at lower exposures (≤2.9 mg-min/L), likely due to insufficient oxidation by hydroxyl radicals. The release of intracellular organic matter (IOM) was also measured to determine the potential of bulk measurements to act as a surrogate for cyanotoxins and metabolite release. In all cases, the dissolved organic carbon (DOC) release was less than 0.25 mgC/L, which lacked the sensitivity to indicate the release of MC-LR, MIB, or geosmin. The fluorescence index proved to be a more sensitive indicator of intracellular organic matter release than DOC for MA. These results illustrate that toxic or odorous compounds may be released from cyanobacteria cells during oxidation processes with minimal changes in the DOC concentration.

  20. Ferrocenylaniline based amide analogs of methoxybenzoic acids: Synthesis, structural characterization and butyrylcholinesterase (BChE) inhibition studies

    Science.gov (United States)

    Altaf, Ataf Ali; Kausar, Samia; Hamayun, Muhammad; Lal, Bhajan; Tahir, Muhammad Nawaz; Badshah, Amin

    2017-10-01

    Three new ferrocene based amides were synthesized with slight structural difference. The general formula of the amides is C5H5FeC5H4C6H4NHCOC6H4(OCH3). The synthesized compounds were characterized by instrumental techniques like elemental analysis, FTIR and NMR spectroscopy. Structure of the two compounds was also studied by single crystal X-rays diffraction analysis. Structural studies provide the evidence that pMeO (one of the synthesized compounds) is an example of amides having no intermolecular hydrogen bonding in solid structure. In the BChE inhibition assay, compound (oMeO) having strong intermolecular force in the solid structure is less active than the compound (pMeO) with weak intermolecular forces in the solid structure. The docking studies proved that hydrogen bonding between inhibitor and BChE enzyme is of more importance for the activity, rather than intermolecular hydrogen bonding in the solid structure of inhibitor.

  1. CMB and BAO constraints for an induced gravity dark energy model with a quartic potential

    CERN Document Server

    Umiltà, Caterina; Finelli, Fabio; Paoletti, Daniela

    2015-01-01

    We study the predictions for structure formation in an induced gravity dark energy model with a quartic potential. By developing a dedicated Einstein-Boltzmann code, we study self-consistently the dynamics of homogeneous cosmology and of linear perturbations without using any parametrization. By evolving linear perturbations with initial conditions in the radiation era, we accurately recover the quasi-static analytic approximation in the matter dominated era. We use Planck 2013 data and a compilation of baryonic acoustic oscillation (BAO) data to constrain the coupling $\\gamma$ to the Ricci curvature and the other cosmological parameters. By connecting the gravitational constant in the Einstein equation to the one measured in a Cavendish-like experiment, we find $\\gamma < 0.0012$ at 95% CL with Planck 2013 and BAO data. This is the tightest cosmological constraint on $\\gamma$ and on the corresponding derived post-Newtonian parameters. Because of a degeneracy between $\\gamma$ and the Hubble constant $H_0$, ...

  2. Numerical solutions of anharmonic vibration of BaO and SrO molecules

    Energy Technology Data Exchange (ETDEWEB)

    Pramudito, Sidikrubadi; Sanjaya, Nugraha Wanda [Theoretical Physics Division, Department of Physics, Bogor Agricultural University, Jalan Meranti Kampus IPB Dramaga Bogor 16680 (Indonesia); Sumaryada, Tony, E-mail: tsumaryada@ipb.ac.id [Theoretical Physics Division, Department of Physics, Bogor Agricultural University, Jalan Meranti Kampus IPB Dramaga Bogor 16680 (Indonesia); Computational Biophysics and Molecular Modeling Research Group (CBMoRG), Department of Physics, Bogor Agricultural University, Jalan Meranti Kampus IPB Dramaga Bogor 16680 (Indonesia)

    2016-03-11

    The Morse potential is a potential model that is used to describe the anharmonic behavior of molecular vibration between atoms. The BaO and SrO molecules, which are two almost similar diatomic molecules, were investigated in this research. Some of their properties like the value of the dissociation energy, the energy eigenvalues of each energy level, and the profile of the wavefunctions in their correspondence vibrational states were presented in this paper. Calculation of the energy eigenvalues and plotting the wave function’s profiles were performed using Numerov method combined with the shooting method. In general we concluded that the Morse potential solved with numerical methods could accurately produce the vibrational properties and the wavefunction behavior of BaO and SrO molecules from the ground state to the higher states close to the dissociation level.

  3. Thermodynamic investigation of ZrO2-BaO system

    Institute of Scientific and Technical Information of China (English)

    GONG Wei-ping; CHEN Teng-fei; JIN Zhan-peng

    2007-01-01

    Thermodynamic description of ZrO2-BaO system was developed using the available experimental information. Special attention was paid to the modelling of the perovskite phase BaZrO3 by a temperature-dependent polynomial to fit the experimental thermodynamic properties. The liquid phases, CSS (cubic ZrO2 solid solution) and TSS (tetragonal ZrO2 solid solution) were modelled with Redlich-Kister formula. The compounds Ba2ZrO4 and Ba3Zr2O7 were treated as stoichiometric phases and the BaOSS and MSS (halite BaO and monoclinic ZrO2 solid solutions) were treated as pure compounds. Comparisons between the calculated and the measured phase diagram as well as the thermodynamic quantities indicate that the most reliable experimental information is satisfactorily accounted for by the present thermodynamic calculation.

  4. Error detection based on MB types

    Institute of Scientific and Technical Information of China (English)

    FANG Yong; JEONG JeChang; WU ChengKe

    2008-01-01

    This paper proposes a method of error detection based on macroblock (MB) types for video transmission. For decoded inter MBs, the absolute values of received residues are accumulated. At the same time, the intra textural complexity of the current MB is estimated by that of the motion compensated reference block. We compare the inter residue with the intra textural complexity. If the inter residue is larger than the intra textural complexity by a predefined threshold, the MB is con-sidered to be erroneous and errors are concealed. For decoded intra MBs, the connective smoothness of the current MB with neighboring MBs is tested to find erroneous MBs. Simulation results show that the new method can remove those seriously-corrupted MBs efficiently. Combined with error concealment, the new method improves the recovered quality at the decoder by about 0.5-1 dB.

  5. Status on bidimensional dark energy parameterizations using SNe Ia JLA and BAO datasets

    CERN Document Server

    Escamilla-Rivera, Celia

    2016-01-01

    Using current observations forecast type Ia supernovae (SNe Ia) Joint Lightcurve Analysis (JLA) binned and baryon acoustic oscillations (BAO), in this paper we investigate six bidimensional dark energy parameterizations in order to explore which has more constraining power. Our results indicate that for Taylor series-like parameterizations at second order in redshift $z$, the tension ($\\sigma$-distance) between these data sets seems to be reduced and their behaviour are $<$1$\\sigma$ compatible with $\\Lambda$CDM.

  6. MB2 in maxillary second molar

    Directory of Open Access Journals (Sweden)

    Prakash R

    2007-01-01

    Full Text Available Occurrence of the second mesiobuccal canal (MB2 is a frequent finding. Literary reports have shown it to be found more in the cases of the maxillary first molar. However the maxillary second molars have also been found with this variation in a number of canals. This paper presents a case report on the occurrence of a second mesiobuccal canal or the MB2 in the maxillary second molar.

  7. Co-operative Wireless Intrusion Detection System Using MIBs From SNMP

    Directory of Open Access Journals (Sweden)

    Ashvini Vyavhare

    2012-03-01

    Full Text Available In emerging technology of Internet, security issues are becoming more challenging. In case of wired LAN it is somewhat in control, but in case of wireless networks due to exponential growth in attacks, it has made difficult to detect such security loopholes. Wireless network security is being addressed using firewalls, encryption techniques and wired IDS (Intrusion Detection System methods. But the approaches which were used in wired network were not successful in producing effective results for wireless networks. It is so because of features of wireless network such as open medium, dynamic changing topology, cooperative algorithms, lack of centralized monitoring and management point, and lack of a clear line of defense etc. So, there is need for new approach which will efficiently detect intrusion in wireless network. Efficiency can be achieved by implementing distributive, co-operative based, multi-agent IDS. The proposed system supports all these three features. It includes mobile agents for intrusion detection which uses SNMP (Simple network Management Protocol and MIB (Management Information Base variables for mobile wireless networks

  8. Formalization, annotation and analysis of diverse drug and probe screening assay datasets using the BioAssay Ontology (BAO.

    Directory of Open Access Journals (Sweden)

    Uma D Vempati

    Full Text Available Huge amounts of high-throughput screening (HTS data for probe and drug development projects are being generated in the pharmaceutical industry and more recently in the public sector. The resulting experimental datasets are increasingly being disseminated via publically accessible repositories. However, existing repositories lack sufficient metadata to describe the experiments and are often difficult to navigate by non-experts. The lack of standardized descriptions and semantics of biological assays and screening results hinder targeted data retrieval, integration, aggregation, and analyses across different HTS datasets, for example to infer mechanisms of action of small molecule perturbagens. To address these limitations, we created the BioAssay Ontology (BAO. BAO has been developed with a focus on data integration and analysis enabling the classification of assays and screening results by concepts that relate to format, assay design, technology, target, and endpoint. Previously, we reported on the higher-level design of BAO and on the semantic querying capabilities offered by the ontology-indexed triple store of HTS data. Here, we report on our detailed design, annotation pipeline, substantially enlarged annotation knowledgebase, and analysis results. We used BAO to annotate assays from the largest public HTS data repository, PubChem, and demonstrate its utility to categorize and analyze diverse HTS results from numerous experiments. BAO is publically available from the NCBO BioPortal at http://bioportal.bioontology.org/ontologies/1533. BAO provides controlled terminology and uniform scope to report probe and drug discovery screening assays and results. BAO leverages description logic to formalize the domain knowledge and facilitate the semantic integration with diverse other resources. As a consequence, BAO offers the potential to infer new knowledge from a corpus of assay results, for example molecular mechanisms of action of perturbagens.

  9. Formalization, annotation and analysis of diverse drug and probe screening assay datasets using the BioAssay Ontology (BAO).

    Science.gov (United States)

    Vempati, Uma D; Przydzial, Magdalena J; Chung, Caty; Abeyruwan, Saminda; Mir, Ahsan; Sakurai, Kunie; Visser, Ubbo; Lemmon, Vance P; Schürer, Stephan C

    2012-01-01

    Huge amounts of high-throughput screening (HTS) data for probe and drug development projects are being generated in the pharmaceutical industry and more recently in the public sector. The resulting experimental datasets are increasingly being disseminated via publically accessible repositories. However, existing repositories lack sufficient metadata to describe the experiments and are often difficult to navigate by non-experts. The lack of standardized descriptions and semantics of biological assays and screening results hinder targeted data retrieval, integration, aggregation, and analyses across different HTS datasets, for example to infer mechanisms of action of small molecule perturbagens. To address these limitations, we created the BioAssay Ontology (BAO). BAO has been developed with a focus on data integration and analysis enabling the classification of assays and screening results by concepts that relate to format, assay design, technology, target, and endpoint. Previously, we reported on the higher-level design of BAO and on the semantic querying capabilities offered by the ontology-indexed triple store of HTS data. Here, we report on our detailed design, annotation pipeline, substantially enlarged annotation knowledgebase, and analysis results. We used BAO to annotate assays from the largest public HTS data repository, PubChem, and demonstrate its utility to categorize and analyze diverse HTS results from numerous experiments. BAO is publically available from the NCBO BioPortal at http://bioportal.bioontology.org/ontologies/1533. BAO provides controlled terminology and uniform scope to report probe and drug discovery screening assays and results. BAO leverages description logic to formalize the domain knowledge and facilitate the semantic integration with diverse other resources. As a consequence, BAO offers the potential to infer new knowledge from a corpus of assay results, for example molecular mechanisms of action of perturbagens.

  10. Mechanisms for O2 dissociation over the BaO (100) surface

    Science.gov (United States)

    Lu, Nai-Xia; Fu, Gang; Xu, Xin; Wan, Hui-Lin

    2008-01-01

    We have investigated the atomic and molecular oxygen adsorptions on the various sites of the BaO (100) surface with both cluster models and the periodic slab models. We found that the atomic oxygen prefers to adsorb on the surface O2- to form the closed-shell peroxides with the binding energies of 83-88kcal/mol. Such a high exothermicity provides a large driving force for the dissociation of molecular O2 on the BaO surfaces. As molecular oxygen approaches the BaO surfaces, the triplet ground state O2 molecule first binds electrostatically on top of the surface Ba2+ site. It further quenches to the singlet potential energy surface to form a covalently bonded O32- species. We proposed a plausible pathway in which the O32- species acts as the key precursor for further dissociation, leading eventually to the formation of surface peroxides O22-. This mechanism is helpful for the understanding of a series of related catalytic processes such as the oxidative coupling of methane, the NOx storage reduction, etc.

  11. Mobile Banking Short Message BCH - NAF - RSA Fast Compilation and Encryption and Security Implementation%手机银行短信息的BCH-NAF-RSA快速编译与加密及其安全实现

    Institute of Scientific and Technical Information of China (English)

    邓从政

    2015-01-01

    The rapid spread of the mobile communication technology promotes the communication operators continue to develop new value-added business such as mobile phone banking to increase profits. These operations are often completed by fast compiled code through the short message center. This paper improved traditional binary multiplication algorithm, and presents an optimized low storage NAF point compression multiplication algorithm. It greatly reduces the running time of point multiplication operation, and saves storage space. Here introduces an optimization of BCH iterative decoding algorithm for the design and realization of mobile phone short message paging decoder. The error rate of this decoder algorithm is small. That can greatly enhance the error correcting ability, and improve the quality of short message sending. Through twice compilation of NAF and BCH, it can use conic curve cryptography to encrypt fastly. The velocity of mobile phone SMS paging signal outgoing and incoming will get greatly improved. It can make sure the safety of mobile phone short message bank business transactions and implementation speed.%智能手机的快速普及推动着通信运营商不断开发新的诸如手机银行类的增值业务以增长利润, 这些业务往往通过短信中心来完成. 为了安全快速的实现这些业务, 这里改进了信号发射台短信息的编译码算法, 引入一种优化的 BCH 迭代译码算法, 来设计和实现手机短信寻呼台译码器, 这种译码算法错码率较小, 能大大增强纠错能力, 提高了短信的发送质量. 同时对短信息的加密和解密算法进行了优化, 提出了一种优化的低存储NAF点压缩数乘算法, 大大地缩短了点乘运算的运行时间, 节约了存储空间, 经过 NAF 和 BCH 快速编译后, 利用安全性较高的基于圆锥曲线的 RSA 公钥密码来进行加密, 以提高手机短信寻呼台信号呼出和呼入的速度及其安全性, 保证了手机银行短

  12. Bcl-2 protein expression is associated with p27 and p53 protein expressions and MIB-1 counts in breast cancer

    Directory of Open Access Journals (Sweden)

    Nishizaki Takashi

    2006-07-01

    Full Text Available Abstract Background Recent experimental studies have shown that Bcl-2, which has been established as a key player in the control of apoptosis, plays a role in regulating the cell cycle and proliferation. The aim of this study was to investigate the relationship between Bcl-2 and p27 protein expression, p53 protein expression and the proliferation activity as defined by the MIB-1 counts. The prognostic implication of Bcl-2 protein expression in relation to p27 and p53 protein expressions and MIB-1 counts for breast cancer was also evaluated. Methods The immunohistochemical expression of Bcl-2 protein was evaluated in a series of 249 invasive ductal carcinomas of the breast, in which p27 and p53 protein expressions and MIB-1 counts had been determined previously. Results The Bcl-2 protein expression was found to be decreased in 105 (42% cases. A decreased Bcl-2 protein expression was significantly correlated with a nuclear grade of III, a negative estrogen receptor, a decreased p27 protein expression, a positive p53 protein expression, positive MIB-1 counts and a positive HER2 protein expression. The incidence of a nuclear grade of III and positive MIB-1 counts increased as the number of abnormal findings of Bcl-2, p27 and p53 protein expressions increased. A univariate analysis indicated a decreased Bcl-2 protein expression to be significantly (p = 0.0089 associated with a worse disease free survival (DFS, while a multivariate analysis indicated the lymph node status and MIB-1 counts to be independently significant prognostic factors for the DFS. Conclusion The Bcl-2 protein expression has a close correlation with p27 and p53 protein expressions and the proliferation activity determined by MIB-1 counts in invasive ductal carcinoma of the breast. The prognostic value of Bcl-2 as well as p27 and p53 protein expressions was dependent on the proliferation activity in breast cancer.

  13. The bchU gene of Chlorobium tepidum encodes the C-20 methyltransferase in bacteriochlorophyll c biosynthesis

    DEFF Research Database (Denmark)

    Maresca, Julia A; Gomez Maqueo Chew, Aline; Ponsatí, Marta Ros

    2004-01-01

    Bacteriochlorophylls (BChls) c and d, two of the major light-harvesting pigments in photosynthetic green sulfur bacteria, differ only by the presence of a methyl group at the C-20 methine bridge position in BChl c. A gene potentially encoding the C-20 methyltransferase, bchU, was identified...

  14. MB3a Infrasound Sensor Evaluation.

    Energy Technology Data Exchange (ETDEWEB)

    Merchant, Bion J.; McDowell, Kyle D.

    2014-11-01

    Sandia National Laboratories has tested and evaluated a new infrasound sensor, the MB3a, manufactured by Seismo Wave. These infrasound sensors measure pressure output by a methodology developed by researchers at the French Alternative Energies and Atomic Energy Commission (CEA) and the technology was recently licensed to Seismo Wave for production and sales. The purpose of the infrasound sensor evaluation was to determine a measured sensitivity, transfer function, power, self-noise, dynamic range, seismic sensitivity, and self- calibration ability. The MB3a infrasound sensors are being evaluated for potential use in the International Monitoring System (IMS) of the Comprehensive Nuclear Test-Ban-Treaty Organization (CTBTO).

  15. Argyrophilic nucleolar organizer region in MIB-1 positive cells in non-small cell lung cancer:clinicopathological signiifcance and sur vival

    Institute of Scientific and Technical Information of China (English)

    Dmitriy Sergeevich Kobyakov; Ashot Merudzhanovich Avdalyan; Aleksandr Fedorovich Lazarev; Elena Leonidovna Lushnikova; Lev Moiseevich Nepomnyashchikh

    2014-01-01

    Objective:To evaluate the relation between argyrophilic nucleolar organizer region (AgNOR)-associated proteins and clinicopathological parameters and survival in non-small-cell lung cancer (NSCLC). Methods:A total of 207 surgical specimens diagnosed as NSCLC were included in this study. Double-staining procedures were performed using antigen Ki-67 (clone MIB-1) and silver nitrate by immunohistochemical and AgNOR-staining methods. Results:The AgNOR area in MIB-1-positive cells of NSCLC is related to clinicopathological parameters under the TNM (tumor, node, and metastasis) system. The survival of patients with small AgNOR area in MIB-1-positive cells is better than that of patients with large AgNOR area. Molecular, biological (AgNOR area in MIB-1-positive cells), and clinicopathological (greatest tumor dimension, metastases to regional lymph nodes, histology, and differentiation) parameters are independent prognostic factors of NSCLC. Conclusion:hTe AgNOR area in MIB-1-positive cells is related to clinicopathological parameters and survival in NSCLC.

  16. Comparison of Adsorption Capability of Activated Carbon and Metal Doped TiO2 for Geosmin and 2-MIB Removal from Water

    Directory of Open Access Journals (Sweden)

    Aisha Asghar

    2015-01-01

    Full Text Available This study stemmed from consumer complaints about earthy and musty off-flavours in treated water of Rawal Lake Filtration Plant. In recent years, several novel adsorbents have been developed from nanomaterials for enhancing the contaminant removal efficiency. This paper presents preparation and the use of new adsorbents Pt doped titania and Fe doped titania, for the adsorption capacity of Geosmin and 2-MIB from water under laboratory conditions and their comparison, with most widely used activated carbon, under batch and column experiments. Stock solutions were prepared by using Geosmin and 2-MIB standards, procured by Sigma Aldrich (England. Samples were analysed using SPME-GC-FID. The adsorption of Geosmin and 2-MIB on GAC conformed to the Freundlich isotherm, while that of adsorption on metal doped titania fit equally well to both Langmuir and Freundlich isotherms. Moreover, data, generated for the kinetic isotherm, confirmed that Geosmin and 2-MIB removal is a function of contact time. Breakthrough column tests using 125 mg/L Pt doped titania nanoparticles, coated on glass beads against 700 ng/L of off-flavours, attained later breakthrough and exhaustion points and removed 98% of Geosmin and 97% of 2-MIB at room temperature. All columns could be regenerated using 50 mL 0.1 molar sodium hydroxide.

  17. EZH2 expression in gliomas: Correlation with CDKN2A gene deletion/ p16 loss and MIB-1 proliferation index.

    Science.gov (United States)

    Purkait, Suvendu; Sharma, Vikas; Jha, Prerana; Sharma, Mehar Chand; Suri, Vaishali; Suri, Ashish; Sharma, B S; Sarkar, Chitra

    2015-10-01

    Enhancer of zeste homolog 2 (EZH2) mediated down-regulation of CDKN2A/p16 has been observed in cell lines as well as in a few carcinomas. However, there is no study correlating EZH2 expression with CDKN2A/p16 status in gliomas. Hence, the present study was conducted to evaluate EZH2 expression in astrocytic and oligodendroglial tumors and correlate with CDKN2A/p16 status as well as MIB-1 labeling index (LI). Gliomas of all grades (n = 118) were studied using immunohistochemistry to assess EZH2, p16 and MIB-1 LI and fluorescence in situ hybrization to evaluate CDKN2A gene status. EZH2 expression and CDKN2A homozygous deletion (HD) were both significantly more frequent in high-grade gliomas (HGG). Further, strong EZH2 expression (LI ≥ 25%) was significantly more common in HGGs without CDKN2A HD (48.7%; 19/39) as compared to cases with deletion (15.8%; 3/19). Loss of p16 expression was noted in 100% and 51.3% of CDKN2A deleted and non-deleted tumors, respectively. Notably, 80% (16/20) of the CDKN2A non-deleted HGGs with p16 loss had strong EZH2 expression, in contrast to only 15.8% (3/19) in the deleted group. Loss of p16 expression significantly correlated with MIB-1 LI, irrespective of EZH2 status. Thus, this study shows that EZH2 expression correlates with tumor grade in both astrocytic and oligodendroglial tumors and hence can be used as a diagnostic marker to differentiate between low and HGGs. Further, this is the first report demonstrating an inverse correlation of strong EZH2 expression with CDKN2A HD in HGGs. Loss of p16 protein expression is mostly attributable to CDKN2A HD and correlates significantly with MIB-1 LI. Notably, our study for the first time suggests a possible epigenetic mechanism of p16 loss in CDKN2A non-deleted HGGs mediated by strong EZH2 expression. A hypothetical model for control of proliferative activity in low versus HGGs is therefore proposed. © 2015 Japanese Society of Neuropathology.

  18. Effect of Natural Organic Matter on Adsorption of MIB and GSM%NOM在去除MIB、GSM上的作用

    Institute of Scientific and Technical Information of China (English)

    曾慧; 王春娥; 王锐

    2009-01-01

    文章介绍了引起饮用水臭味的痕最污染物土臭素(geosmin,GSM)和二甲基异冰片(2-methylisoborneol,MIB)的来源和危害.阐述了臭氧氧化、活性炭吸附去除MIB、GSM时的主要影响因素,分析了各因素对去除率的影响程度及原因.

  19. Optimized Clustering Estimators for BAO Measurements Accounting for Significant Redshift Uncertainty

    Energy Technology Data Exchange (ETDEWEB)

    Ross, Ashley J. [Portsmouth U., ICG; Banik, Nilanjan [Fermilab; Avila, Santiago [Madrid, IFT; Percival, Will J. [Portsmouth U., ICG; Dodelson, Scott [Fermilab; Garcia-Bellido, Juan [Madrid, IFT; Crocce, Martin [ICE, Bellaterra; Elvin-Poole, Jack [Jodrell Bank; Giannantonio, Tommaso [Cambridge U., KICC; Manera, Marc [Cambridge U., DAMTP; Sevilla-Noarbe, Ignacio [Madrid, CIEMAT

    2017-05-15

    We determine an optimized clustering statistic to be used for galaxy samples with significant redshift uncertainty, such as those that rely on photometric redshifts. To do so, we study the BAO information content as a function of the orientation of galaxy clustering modes with respect to their angle to the line-of-sight (LOS). The clustering along the LOS, as observed in a redshift-space with significant redshift uncertainty, has contributions from clustering modes with a range of orientations with respect to the true LOS. For redshift uncertainty $\\sigma_z \\geq 0.02(1+z)$ we find that while the BAO information is confined to transverse clustering modes in the true space, it is spread nearly evenly in the observed space. Thus, measuring clustering in terms of the projected separation (regardless of the LOS) is an efficient and nearly lossless compression of the signal for $\\sigma_z \\geq 0.02(1+z)$. For reduced redshift uncertainty, a more careful consideration is required. We then use more than 1700 realizations of galaxy simulations mimicking the Dark Energy Survey Year 1 sample to validate our analytic results and optimized analysis procedure. We find that using the correlation function binned in projected separation, we can achieve uncertainties that are within 10 per cent of of those predicted by Fisher matrix forecasts. We predict that DES Y1 should achieve a 5 per cent distance measurement using our optimized methods. We expect the results presented here to be important for any future BAO measurements made using photometric redshift data.

  20. The sensitivity of BAO dark energy constraints to general isocurvature perturbations

    Energy Technology Data Exchange (ETDEWEB)

    Kasanda, S. Muya; Zunckel, C.; Moodley, K. [School of Mathematical Sciences, University of KwaZulu-Natal, University Road, Durban, 4041 (South Africa); Bassett, B.A.; Okouma, P., E-mail: simon.muya.kasanda@gmail.com, E-mail: caroline.zunckel@gmail.com, E-mail: kavilan.moodley@gmail.com, E-mail: bruce.a.bassett@gmail.com, E-mail: okouma@gmail.com [Dept. of Mathematics and Applied Mathematics, University of Cape Town, Rondebosch 7701, Cape Town (South Africa)

    2012-07-01

    Baryon Acoustic Oscillation (BAO) surveys will be a leading method for addressing the dark energy challenge in the next decade. We explore in detail the effect of allowing for small amplitude admixtures of general isocurvature perturbations in addition to the dominant adiabatic mode. We find that non-adiabatic initial conditions leave the sound speed unchanged but instead excite different harmonics. These harmonics couple differently to Silk damping, altering the form and evolution of acoustic waves in the baryon-photon fluid prior to decoupling. This modifies not only the scale on which the sound waves imprint onto the baryon distribution, which is used as the standard ruler in BAO surveys, but also the shape, width and height of the BAO peak. We discuss these effects in detail and show how more general initial conditions impact our interpretation of cosmological data in dark energy studies. We find that the inclusion of these additional isocurvature modes leads to a decrease in the Dark Energy Task Force figure of merit (FoM) by 46% i.e., FoM{sub ISO} = 0.54 × FoM{sub AD} and 53% for the BOSS and ADEPT experiments respectively when considered in conjunction with PLANK data. We also show that the incorrect assumption of adiabaticity has the potential to bias our estimates of the dark energy parameters by 2.7σ (2.2σ) for a single correlated isocurvature mode (CDM isocurvature), and up to 4.9σ (5.7σ) for three correlated isocurvature modes in the case of the BOSS (ADEPT) experiment. We find that the use of the large scale structure data in conjunction with CMB data improves our ability to measure the contributions of different modes to the initial conditions by as much as 95% for certain modes in the fully correlated case.

  1. Sanggar Seni “Bao Daya” di Lombok Timur Menyongsong Industri Pariwisata

    Directory of Open Access Journals (Sweden)

    Yosef Adityanto Aji

    2014-11-01

    Full Text Available Sanggar Seni “Bao Daya” di Desa Lenek Tengah Kabupaten Lombok Timurmerupakan kelompok kesenian yang sangat membantu program pemerintah untukmenunjukkan jati diri kesenian. Semua seni pertunjukan yang ditampilkan untukmenjamu wisatawan berakar dari budaya masyarakat setempat. Keunikan dankeunggulan produk berusaha ditampilkan oleh kelompok ini sebagai citra darisuatu daerah. Metode kualitatif digunakan untuk melihat fenomena yang terjadidan berkembang pada situasi sosial di sanggar-sanggar seni di desa Lenek Tengah.Hasil penelitian ini memperlihatkan bahwa sanggar seni “Bao Daya” memberikankonstribusi yang besar terhadap kehidupan pariwisata dan kelangsungan hidupanggota masyarakat di sekitarnya selain itu keterlibatan pengelola seni besertaanggota keluarganya menjadi faktor utama dari keberlanjutan dan regenerasi senitradisi di Lombok Timur. Keberlanjutan hidup kelompok kesenian ini selainbergantung pada aktivitas anggota untuk selalu menyiapkan seni pertunjukan yangditampilkan juga ketersediaan sumber daya manusia sebagai pelakunya. Sejalandengan itu, kelangsungannya tergantung pula pada kehadiran wisatawan danpengelolaan organisasi secara profesional. The Art Studio “Bao Daya” in East Lombok - Welcome the Tourism Industry.The activities of the art studio “Bao Daya” in the village of East Lombok, Lenek Tengah,are very helpful to support the local government programs showing the identity of arts.All performing arts which are performed for tourists to entertain rooted from the localculture. The uniqueness and superiority of products are showed by this studio as theimage of a certain area. However, the studio not only shows the performances that serveas the performance for the tourists, but also is aimed to create service products that areutilized by the supporters of certain community.Although the management orientation tends to meet the needs of economy, the coachingand regeneration are not totally ignored. Trainings are

  2. Cosmology in doubly coupled massive gravity: Constraints from SNIa, BAO and CMB

    Science.gov (United States)

    Heisenberg, Lavinia; Refregier, Alexandre

    2016-11-01

    Massive gravity in the presence of doubly coupled matter field via en effective composite metric yields an accelerated expansion of the universe. It has been recently shown that the model admits stable de Sitter attractor solutions and could be used as a dark energy model. In this work, we perform a first analysis of the constraints imposed by the SNIa, BAO and CMB data on the massive gravity model with the effective composite metric and show that all the background observations are mutually compatible at the one sigma level with the model.

  3. Cosmology in doubly coupled massive gravity: constraints from SNIa, BAO and CMB

    CERN Document Server

    Heisenberg, Lavinia

    2016-01-01

    Massive gravity in the presence of doubly coupled matter field via en effective composite metric yields an accelerated expansion of the universe. It has been recently shown that the model admits stable de Sitter attractor solutions and could be used as a dark energy model. In this work, we perform a first analysis of the constraints imposed by the SNIa, BAO and CMB data on the massive gravity model with the effective composite metric and show that all the background observations are mutually compatible at the one sigma level with the model.

  4. Delayed Feedback Control of Bao Chaotic System Based on Hopf Bifurcation Analysis

    Directory of Open Access Journals (Sweden)

    Farhad Khellat

    2014-11-01

    Full Text Available This paper is concerned with bifurcation and chaos control in a new chaotic system recently introduced by Bao et al [9]. First a condition that the system has a Hopf bifurcation is derived. Then by applying delayed feedback controller, the chaotic system is forced to have a stable periodic orbit extracting from chaotic attractor. This is done by making Hopf bifurcation value of the open loop and the closed loop systems identical. Also by suitable tuning of the controller parameters, unstable equilibrium points become stable. Numerical simulations verify the results.

  5. 10 MB disk platter from CDC 7638

    CERN Multimedia

    1974-01-01

    This magnetic disk was one of three which interfaced with various Control Data machines. This single platter came from a Control Data 7638 Disk Storage Subsystem and could contain up to 10MB - about the size of a few MP4's on your iPod.

  6. The expression of P16 INK4A and MIB-1 in cervical cancer and cervical cancer lesion before%P16INK4A与MIB-1在宫颈癌及宫颈癌前病变中的表达

    Institute of Scientific and Technical Information of China (English)

    王志新; 肖明明

    2011-01-01

    目的:探讨P16 INK4A与MIB-1在宫颈癌及宫颈癌前病变中的表达情况.方法:采用免疫组织化学方法检测P16 INK4A与MIB-1在63例宫颈组织及38例宫颈涂片中的表达.结果:P16在正常宫颈上皮中均无表达.随着宫颈病变级别的增高,其阳性表达率逐渐增大,其中,宫颈癌中P16的表达增强明显.MIB-1随着宫颈病变级别的增高,表达增强,其中,宫颈癌中MIB-1的表达增强明显.在宫颈病变组织中,随着P16阳性表达率的增大,MIB-1表达也相应增高,具有相关性.结论:宫颈上皮内病变中P16及MIB-1过表达,提示两者可以作为宫颈病变的诊断标志物.在宫颈病变及宫颈癌的确切诊断中,P16及MIB-1可以作为诊断依据之一.%Objective: To evaluate the expression of P16 INK4A and MIB-1 in cervical cancer and cervical cancer lesion before.Methods: Immunohistochemistry methods were used to detect the expression of P16 INK4A and MIB-1 in 63 cases of cervical tissue and 38 cases of cerical smear.Results: No positive staining of P16 was observed in the normal cervical epithelium.With increasing severity of cervical intraepithelial neoplasias (CIN), the P16 expression increased progressively, significant up-regulation of P16 was observed in carcinoma cervix.MIB-1 was observed with increasing severity of CIN, and significant overexpression of MIB-1 was observed in carcinoma cervix.The expression of MIB-1 was increased with consistently increasing of P16, there was a correlation between P16 and MIB-1 in cervical lesions organization.Conclusion: The overexpression of P16 and MIB-1 demonstrates that they can be used as a diagnostic marker for cervical lesions.Therefore, in the exact diagnosis of cervical lesions and cervical cancer, P16 and MIB-1 markers can be used as one of the diagnostic bases.

  7. Ability of humans to smell geosmin, 2-MIB and nonadienal in indoor air when using contaminated drinking water.

    Science.gov (United States)

    Omür-Ozbek, P; Little, J C; Dietrich, A M

    2007-01-01

    The most common compounds responsible for off flavours are geosmin, 2-MIB, and nonadienal which are poorly removed by conventional water treatment operations and hence result in customer complaints. Because these odourants are moderately volatile and have very low odour threshold values, it is necessary to determine their concentrations in air when water is used indoors. If the detectable aqueous concentrations for these odourants are known, the utilities may take action to treat their water at times when the concentration of the raw water exceeds the threshold concentration. To predict the concentration in the shower stall and bathroom air after showering, recently published Henry's law constants for the selected odourants and a model developed to determine the volatilization of the odourous compounds by applying two-resistance theory were used. Then the results were compared with the odour threshold concentration data to determine under which conditions the odourants become detectable. For parameters representing a typical bathroom and shower stall setting, the results showed that the odourants become detectable when the aqueous concentration of geosmin and nonadienal exceed 10 ng/L at 42 degrees C. As the aqueous concentration increases, geosmin and nonadienal become detectable at lower temperatures, however 2-MIB is only detectable above 20 ng/L and at 42 degrees C.

  8. Constraints on reconstructed dark energy model from SN Ia and BAO/CMB observations

    CERN Document Server

    Mamon, Abdulla Al; Das, Sudipta

    2016-01-01

    The motivation of the present work is to reconstruct a dark energy model through the dimensionless dark energy function X(z), which is the dark energy density in units of its present value. In this paper, we have tried to show that a scalar field {\\phi} having a phenomenologically chosen X(z) can give rise to a transition from a decelerated to an accelerated phase of expansion for the universe. We have examined the possibility of constraining various cosmological parameters (such as the deceleration parameter and the effective equation of state parameter) by comparing our theoretical model with the latest Type Ia Supernova (SN Ia), Baryon Acoustic Oscillations (BAO) and Cosmic Microwave Background (CMB) radiation observations. Using the joint analysis of the SN Ia+BAO/CMB dataset, we have also reconstructed the scalar potential from the parametrized X(z). The relevant potential is found, which comes to be a polynomial in {\\phi}. Finally, the behavior of the distance modulus against redshift has also been inve...

  9. Bayesian comparison of nonstandard cosmologies using type Ia supernovae and BAO data

    Science.gov (United States)

    Santos, B.; Devi, N. Chandrachani; Alcaniz, J. S.

    2017-06-01

    We use the most recent type Ia supernovae (SNe Ia) observations to perform a statistical comparison between the standard Λ CDM model and its extensions [w CDM and w (z )CDM ] and some alternative cosmologies: namely, the Dvali-Gabadadze-Porrati (DGP) model, a power-law f (R ) scenario in the metric formalism and an example of vacuum decay [Λ (t )CDM ] cosmology in which the dilution of pressureless matter is attenuated with respect to the usual a-3 scaling due to the interaction of the dark matter and dark energy fields. We perform a Bayesian model selection analysis using the MultiNest algorithm. To obtain the posterior distribution for the parameters of each model, we use the joint light-curve analysis (JLA) SNe Ia compilation containing 740 events in the interval 0.01 data are analyzed with the SALT2 light-curve fitter and the model selection is then performed by computing the Bayesian evidence of each model and the Bayes factor of the Λ CDM cosmology related to the other models. The results indicate that the JLA data alone are unable to distinguish the standard Λ CDM model from some of its alternatives but its combination with current measurements of baryon acoustic oscillations shows up an ability to distinguish them. In particular, the DGP model is practically not supported by both the BAO and the joint JLA +BAO data sets compared to the standard scenario. Finally, we provide a rank order for the models considered.

  10. Constraints on reconstructed dark energy model from SN Ia and BAO/CMB observations

    Science.gov (United States)

    Mamon, Abdulla Al; Bamba, Kazuharu; Das, Sudipta

    2017-01-01

    The motivation of the present work is to reconstruct a dark energy model through the dimensionless dark energy function X( z), which is the dark energy density in units of its present value. In this paper, we have shown that a scalar field φ having a phenomenologically chosen X( z) can give rise to a transition from a decelerated to an accelerated phase of expansion for the universe. We have examined the possibility of constraining various cosmological parameters (such as the deceleration parameter and the effective equation of state parameter) by comparing our theoretical model with the latest Type Ia Supernova (SN Ia), Baryon Acoustic Oscillations (BAO) and Cosmic Microwave Background (CMB) radiation observations. Using the joint analysis of the SN Ia+BAO/CMB dataset, we have also reconstructed the scalar potential from the parametrized X( z). The relevant potential is found, a polynomial in φ . From our analysis, it has been found that the present model favors the standard Λ CDM model within 1σ confidence level.

  11. Reversible Decomposition of Secondary Phases in BaO Infiltrated LSM Electrodes-Polarization Effects

    DEFF Research Database (Denmark)

    Traulsen, Marie Lund; McIntyre, Melissa D.; Norrman, Kion

    2016-01-01

    In operando Raman spectroscopy is used to study ceramic La0.85Sr0.15MnO3±δ electrodes infiltrated with BaO. The aim of this work is to clarify why BaO infiltration reduces the polarization resistance in oxygen containing atmospheres. Prior to the in operando experiments, ex situ X-ray diffraction...... and Raman spectroscopy reveal the formation of a secondary phase, Ba3Mn2O8, on the electrode. During the in operando Raman investigation of the BaO-infiltrated La0.85Sr0.15MnO3±δ electrodes, experiments are performed at 300 and 500 °C with oxygen partial pressure 0.1 atm and with −1 or +1 V Applied...... potential. A changing electrode surface is observed during operation as the Ba3Mn2O8 secondary phase decomposes and manganese oxide accumulates on the electrode surface during cathodic polarization. The observed changes are reversible. These results suggest that the formation of Ba3Mn2O8 is responsible...

  12. Constraints on reconstructed dark energy model from SN Ia and BAO/CMB observations

    Energy Technology Data Exchange (ETDEWEB)

    Mamon, Abdulla Al [Manipal University, Manipal Centre for Natural Sciences, Manipal (India); Visva-Bharati, Department of Physics, Santiniketan (India); Bamba, Kazuharu [Fukushima University, Division of Human Support System, Faculty of Symbiotic Systems Science, Fukushima (Japan); Das, Sudipta [Visva-Bharati, Department of Physics, Santiniketan (India)

    2017-01-15

    The motivation of the present work is to reconstruct a dark energy model through the dimensionless dark energy function X(z), which is the dark energy density in units of its present value. In this paper, we have shown that a scalar field φ having a phenomenologically chosen X(z) can give rise to a transition from a decelerated to an accelerated phase of expansion for the universe. We have examined the possibility of constraining various cosmological parameters (such as the deceleration parameter and the effective equation of state parameter) by comparing our theoretical model with the latest Type Ia Supernova (SN Ia), Baryon Acoustic Oscillations (BAO) and Cosmic Microwave Background (CMB) radiation observations. Using the joint analysis of the SN Ia+BAO/CMB dataset, we have also reconstructed the scalar potential from the parametrized X(z). The relevant potential is found, a polynomial in φ. From our analysis, it has been found that the present model favors the standard ΛCDM model within 1σ confidence level. (orig.)

  13. Digital Technology in the protection of cultural heritage Bao Fan Temple mural digital mapping survey

    Science.gov (United States)

    Zheng, Y.

    2015-08-01

    Peng Xi county, Sichuan province, the Bao Fan temple mural digitization survey mapping project: we use three-dimensional laserscanning, multi-baseline definition digital photography, multi-spectral digital image acquisition and other technologies for digital survey mapping. The purpose of this project is to use modern mathematical reconnaissance mapping means to obtain accurate mural shape, color, quality and other data. Combined with field investigation and laboratory analysis results, and based on a comprehensive survey and study, a comprehensive analysis of the historical Bao Fan Temple mural artistic and scientific value was conducted. A study of the mural's many qualities (structural, material, technique, preservation environment, degradation, etc.) reveal all aspects of the information carried by the Bao Fan Temple mural. From multiple angles (archeology, architecture, surveying, conservation science and other disciplines) an assessment for the Bao Fan Temple mural provides basic data and recommendations for conservation of the mural. In order to achieve the conservation of cultural relics in the Bao Fan Temple mural digitization survey mapping process, we try to apply the advantages of three-dimensional laser scanning equipment. For wall murals this means obtaining three-dimensional scale data from the scan of the building and through the analysis of these data to help determine the overall condition of the settlement as well as the deformation of the wall structure. Survey analysis provides an effective set of conclusions and suggestions for appropriate mural conservation. But before data collection, analysis and research need to first to select the appropriate scanning equipment, set the appropriate scanning accuracy and layout position of stations necessary to determine the scope of required data. We use the fine features of the three-dimensional laser scanning measuring arm to scan the mural surface deformation degradation to reflect the actual state of

  14. MB C220 Centering System Capacitor Fire

    Science.gov (United States)

    Worth, Daniel B.

    2003-01-01

    A detailed analysis of the MB C220 Centering System Capacitor Fire is presented. The topics include: 1) Description of Incident/Mishap; 2) System Block Diagram; 3) Fault Tree Analysis; 4) Inspection and Repair of Cabinet; 5) Discussions with GSFC Experts; 6) Electrical Measurements of Capacitors; 7) Other Research; 8) Discussions with Capacitor Manufacturers; 9) Findings/Root Cause; and 10) Recommendations. This paper is in viewgraph form.

  15. Biochemical and genetic analysis of butyrylcholinesterase (BChE) in a family, due to prolonged neuromuscular blockade after the use of succinylcholine

    Science.gov (United States)

    Garcia, Daniel Fantozzi; Oliveira, Ticiano G.; Molfetta, Greice A.; Garcia, Luiz V.; Ferreira, Cristiane A.; Marques, Adriana A.; Silva, Wilson Araujo

    2011-01-01

    Butyrylcholinesterase (BChE) is a plasma enzyme that catalyzes the hydrolysis of choline esters, including the muscle-relaxant succinylcholine and mivacurium. Patients who present sustained neuromuscular blockade after using succinylcholine usually carry BChE variants with reduced enzyme activity or an acquired BChE deficiency. We report here the molecular basis of the BCHE gene underlying the slow catabolism of succinylcholine in a patient who underwent endoscopic nasal surgery. We measured the enzyme activity of BChE and extracted genomic DNA in order to study the promoter region and all exons of the BCHE gene of the patient, her parents and siblings. PCR products were sequenced and compared with reference sequences from GenBank. We detected that the patient and one of her brothers have two homozygous mutations: nt1615 GCA > ACA (Ala539Thr), responsible for the K variant, and nt209 GAT > GGT (Asp70Gly), which produces the atypical variant A. Her parents and two of her brothers were found to be heterozygous for the AK allele, and another brother is homozygous for the normal allele. Sequence analysis of exon 1 including 5′UTR showed that the proband and her brother are homozygous for –116GG. The AK/AK genotype is considered the most frequent in hereditary hypocholinesterasemia (44%). This work demonstrates the importance of defining the phenotype and genotype of the BCHE gene in patients who are subjected to neuromuscular block by succinylcholine, because of the risk of prolonged neuromuscular paralysis. PMID:21637541

  16. Biodecolorization of azo dye Remazol orange by Pseudomonas aeruginosa BCH and toxicity (oxidative stress) reduction in Allium cepa root cells.

    Science.gov (United States)

    Jadhav, Shekhar B; Surwase, Shripad N; Kalyani, Dayanand C; Gurav, Ranjit G; Jadhav, Jyoti P

    2012-11-01

    In this report a textile azo dye Remazol orange was degraded and detoxified by bacterium Pseudomonas aeruginosa BCH in plain distilled water. This bacterial decolorization performance was found to be pH and temperature dependent with maximum decolorization observed at pH 8 and temperature 30 °C. Bacterium tolerated higher dye concentrations up to 400 mg l(-1). Effect of initial cell mass showed that higher cell mass concentration can accelerate decolorization process with maximum of 92 % decolorization observed at 2.5 g l(-1) cell mass within 6.5 h. Effect of various metal ions showed Mn has inducing effect whereas Zn strongly inhibited the decolorization process at 5 mM concentration. Analysis of biodegradation products carried out with UV-vis spectroscopy, HPTLC and FTIR confirmed the decolorization and degradation of Remazol orange. Possible route for the degradation of dye was proposed based on GC-MS analysis. During toxicological scrutiny in Allium cepa root cells, induction in the activities of superoxide dismutase (SOD), guaiacol peroxidase (GPX) and inhibition of catalase (CAT) along with raised levels of lipid peroxidation and protein oxidation in dye treated samples were detected which conclusively indicated the generation of oxidative stress. Less toxic nature of the dye degraded products was observed after bacterial treatment.

  17. Rapid biodegradation and decolorization of Direct Orange 39 (Orange TGLL) by an isolated bacterium Pseudomonas aeruginosa strain BCH.

    Science.gov (United States)

    Jadhav, Jyoti P; Phugare, Swapnil S; Dhanve, Rhishikesh S; Jadhav, Shekhar B

    2010-06-01

    A newly isolated novel bacterium from sediments contaminated with dyestuff was identified as Pseudomonas aeruginosa strain BCH by 16S rRNA gene sequence analysis. The bacterium was extraordinarily active and operative over a wide rage of temperature (10-60 degrees C) and salinity (5-6%), for decolorization of Direct Orange 39 (Orange TGLL) at optimum pH 7. This strain was capable of decolorizing Direct Orange 39; 50 mg l(-1) within 45 +/- 5 min, with 93.06% decolorization, while maximally it could decolorize 1.5 g l(-1) of dye within 48 h with 60% decolorization. Analytical studies as, UV-Vis spectroscopy, FTIR, HPLC were employed to confirm the biodegradation of dye and formation of new metabolites. Induction in the activities of lignin peroxidases, DCIP reductase as well as tyrosinase was observed, indicating the significant role of these enzymes in biodegradation of Direct Orange 39. Toxicity studies with Phaseolus mungo and Triticum aestivum revealed the non-toxic nature of degraded metabolites.

  18. A Low-Complexity and High-Throughput RTL Design of a BCH (15,7 Decoder

    Directory of Open Access Journals (Sweden)

    Hendra Setiawan

    2013-09-01

    Full Text Available The  Bose,  Chaudhuri  and  Hocquenghem  (BCH  codes  form  a  large class  of  powerful  random-error  correcting  cyclic  codes.  However,  the implementation  of  its  decoder  requires  high-complexity  computation  resources with a huge number of sequential circuits. This paper presents a  low-complexity register transfer level (RTL circuit design of a BCH decoder. In accordance with the  table  relationship  between  the  syndrome  and  the  error  bit  position,  we propose  a  circuit that  is  mostly occupied by combinational elements without any sequential evolvement. Therefore the designed  system has a  low complexity and high  throughput  properties.  The  implementation  of  the  BCH  (15,7decoder  on Virtex  5  FX70TFF1136  requires  77  look-up  tables  (LUTs  with  the  maximum throughput reaching 1.7 Gbps.

  19. The proliferative potential of the pilocytic astrocytoma : The relation between MIB-1 labeling and clinical and neuro-radiological follow-up

    NARCIS (Netherlands)

    Dirven, CMF; Koudstaal, J; Mooij, JJA; Molenaar, WM

    1998-01-01

    The proliferative potential of 39 pilocytic and 5 low grade astrocytomas was studied in relation to the Ki-67 activity as measured by the MIB-1 Labelings Index. The results were correlated to the biological behaviour of the tumor as measured by clinical and neuro-radiological (CT- or MRI-scans) foll

  20. Three self-report questionnaires of the early mother-to-infant bond: reliability and validity of the Dutch version of the MPAS, PBQ and MIBS.

    Science.gov (United States)

    van Bussel, Johan C H; Spitz, Bernard; Demyttenaere, Koen

    2010-10-01

    In this study, we investigated the reliability and validity of three self-report questionnaires measuring the early emotional bond between a mother and her newborn infant: the Maternal Postpartum Attachment Scale (MPAS), the Postpartum Bonding Questionnaire (PBQ) and the Mother-to-Infant Bonding Scale (MIBS). In a monocentric prospective observational cohort study, 263 mothers completed the MPAS, the PBQ and the MIBS at 8-12 and at 20-25 weeks postpartum. The participants also completed measures of mental health and, during their pregnancy, measures of recalled parental bonding, adult romantic attachment, antenatal attachment and social desirability. In our study, the internal reliabilities of the PBQ and the MPAS were high at 8-12 weeks postpartum but dropped significantly at 20-25 weeks postpartum. Moderately strong correlations between the scales of the PBQ, the MPAS and the MIBS supported their construct validity. Further, weak correlations were found with social desirability and adult attachment representations, whereas moderate correlations were found with antenatal feelings of attachment and antenatal attitudes to motherhood. Finally, maternal feelings of bonding were also moderately associated with maternal mood. Overall, our findings suggest that the MPAS, the PBQ and the MIBS provide a reliable and valid indication of the early emotional tie between a woman and her newborn infant.

  1. Effect of BaO on catalytic performance of Pd-based catalysts for purification of gasoline-methanol exhaust

    Institute of Scientific and Technical Information of China (English)

    张雪乔; 赵明; 徐成华; 汪嘉扬; 陈耀强

    2014-01-01

    Barium oxide was developed successfully to modify palladium catalysts supported on CeO2-ZrO2-La2O3-Al2O3 (CZLA) compound oxides by impregnation method. N2 adsorption (BET), X-ray diffraction (XRD), H2-temperature-programmed reduction (H2-TPR) and X-ray photoelectron spectroscopy (XPS) were employed to characterize the influence of BaO on the physicochemical properties of catalyst. And catalytic activity tests for methanol, CO, C3H8 and NO conversion were evaluated. Catalytic activity re-sults showed that BaO had a positive effect on the conversion of all pollutants. H2-TPR results suggested that the addition of BaO in-creased the reductive ability of the palladium catalysts. The XPS results indicated that doping BaO also improved the dispersion of Pd species and increased the amounts of Ce3+on the Pd-Ba/CZLA catalyst surface, which led to a better redox property. The excellent redox property helped to improve the catalytic activities toward all the pollutants over Pd-based catalysts.

  2. Discrepancy in parameter constraints for LTB models using BAO and SNIa

    Science.gov (United States)

    Vargas, C. Z.; Falciano, F. T.; Reis, R. R. R.

    2017-01-01

    In the present work we constrain three different profiles of a Lemaître–Tolman–Bondi model using supernovae type Ia and baryon acoustic oscillation data. We use two distinct parameter estimation approaches, namely, the {χ2} and the complete Likelihood functional. It has been argued that these two approaches are not equivalent and indeed our analysis shows a specific example of their departure. The combined analysis of BAO  +  SNIa offers a stringent test for these models. In addition, we improve common practice in the literature by carefully calibrating the supernovae in the appropriate inhomogeneous background dynamics. We address subtle issues in order to propagate the primordial BAO scale to the present epoch.

  3. A cosmographic analysis of the transition to acceleration using SN-Ia and BAO

    CERN Document Server

    Muthukrishna, Daniel

    2016-01-01

    We explore the distance-redshift relation using a cosmographic methodology, and show how the cosmographic parameters can be used to determine the redshift of transition from deceleration to acceleration. Such a transition at a low redshift occupies only a small region of the available parameter space, and the prior assumption of an early period of deceleration can significantly change the posterior constraints. We use available type Ia Supernovae (SN-Ia) and Baryon Acoustic Oscillation (BAO) data sets to determine the cosmographic deceleration $q_0$, jerk $j_0$, snap $s_0$ and lerk $l_0$ parameters. The parameters are consistent with the $\\Lambda$CDM model for a flat universe within 2-sigma. We derive constraints on the redshift of transition from deceleration to acceleration for the different expansions, and find $z_{\\rm acc} > 0.14$ at 95% confidence in the most conservative case.

  4. NAND flash error correction arithmetic based on ECC embedded BCH code%ECC嵌入BCH码的NAND闪存纠错算法

    Institute of Scientific and Technical Information of China (English)

    李进; 金龙旭; 李国宁; 张珂; 傅瑶; 朱鹏

    2012-01-01

    针对现有闪存基于硬件ECC纠错算法的纠错能力差,而基于RS码和BCH码纠错算法的译码耗时长的问题,提出一种适于空间应用的硬件ECC嵌入BCH码的闪存纠错算法.分析了闪存内部组织结构特点及闪存硬件ECC纠错原理,提出了一种嵌入BCH(2084,2048,3)码的闪存纠错算法.采用一种蝶形阵列处理机制来迭代计算BCH校验码.使用地面检测设备对闪存纠错算法进行了试验验证.结果表明,纠错算法能快速稳定、可靠地工作,在Flash单页2 kB/页下,可以纠正24b错误.该纠错提高了空间相机图像存储系统的可靠性.%In order to resolve the problem of the low error correcting capability of hardware ECC and the being time-consuming of decoder based on RS code or BCH code, a NAND flash error correction algorithm based on hardware ECC embedded BCH code for space application was proposed in this paper. First, this paper presents the structural characteristic of flash. Then the principle of data flash hardware ECC was analyzed. An embedded BCH code error correction arithmetic was put fonvard. Then the check code of BCH encoder was calculated by an 8-bit parallel butterfly array processing mechanism. Finally, the verification experiments to NAND controller in the prototype machine of XX-X space multi-spectral camera were carried out. The experiments results showed that the proposed error correction algorithms can operate fast, efficiently, reliably, and stably. The algorithm is able to correct 24b errors in 2 kB/page. The algorithm can improve the reliability of the space camera data storage.

  5. La-Mb Studies of Sugars

    Science.gov (United States)

    Lozoya, M.; Cabezas, C.; Mata, S.; López, J. C.; Alonso, J. L.

    2011-06-01

    Recently, rotational studies of biomolecules have entered in a new stage with the LA-MB-FTMW experiment. It combines laser ablation with Fourier transform microwave spectroscopy in supersonic jets overcoming the problems of thermal decomposition associated with conventional heating methods. This technique has been successfully applied to the study of monosaccarides. Three conformers of the prototype α-D-glucose and other three for β-D-glucose have been characterized for the first time in the gas phase. J. L. Alonso, C. Pérez, M. E. Sanz, J. C. López, S. Blanco, Phys. Chem. Chem. Phys. 11,617-627 (2009)and references therein.

  6. The 1990 MB: The first Mars Trojan

    Science.gov (United States)

    Bowell, Edward

    1991-01-01

    Asteroid 1990 MB was discovered during the course of the Mars and Earth-crossing Asteroid and Comet Survey. An orbit based on a 9-day arc and the asteroid's location near Mars L5 longitude led to speculation that it might be in 1:1 resonance with Mars, analogous to the Trojan asteroids of Jupiter. Subsequent observations strengthened the possibility, and later calculations confirmed it. The most recent orbit shows that the asteroid's semimajor axis is very similar to that of Mars.

  7. A photoemission study of BaO overlayers adsorbed on W(110) and their interaction with H 2O, CO 2, and O 2

    Science.gov (United States)

    Mueller, D. R.; Kurtz, R. L.; Stockbauer, R. L.; Madey, T. E.; Shih, Arnold

    1990-11-01

    The electronic structure of barium oxide overlayers on W(110) and their interaction with H 2O, CO 2 and O 2 has been examined using ultraviolet photo electron spectroscopy. At room temperature water vapor and carbon dioxide react with a c(5 × 1)-BaO monolayer adsorbed on W(110) to produce adsorbed OH and CO 3 species. Heating the W(110)-c(5 × 1)-BaO surface following water or carbon dioxide exposure dissociates the adsorbed hydroxide or carbonate. On the lower coverage c(7 × 1)-BaO and c(2 × 4)-BaO adlayers complete dissociation of a fraction of the adsorbed species is evident at room temperature. The interaction of oxygen with W(110) is enhanced by the presence of barium oxide on the tungsten surface. These results are compared with the findings of a previous study of barium oxide films adsorbed on W(100).

  8. Assessment of growth potential by MIB-1 immunohistochemistry in ameloblastic fibroma and related lesions of the jaws compared with ameloblastic fibrosarcoma.

    Science.gov (United States)

    Sano, K; Yoshida, S; Ninomiya, H; Ikeda, H; Ueno, K; Sekine, J; Iwamoto, H; Uehara, M; Inokuchi, T

    1998-02-01

    Specimens from two ameloblastic fibromas (including one recurrent case), two ameloblastic fibro-odontomas, and one ameloblastic fibrosarcoma were subjected to investigation by MIB-1 immunohistochemistry in order to elucidate the growth potential of these tumors. MIB-1 labeling indices in the epithelial component of these tumors ranged from 2.9 to 7.5%, whereas those in the mesenchymal component ranged from 1.5 to 13.5%. Of these, labeling indices in the mesenchymal component of the recurrent ameloblastic fibroma and ameloblastic fibrosarcoma were quite high. These findings suggest that evaluation of growth potential in ameloblastic fibroma and related lesions could be of help in understanding tumor aggressiveness and in selecting appropriate surgical procedures.

  9. In vivo assessment of the antiproliferative properties of interferon-alpha during immunotherapy: Ki-67 (MIB-1) in patients with metastatic renal cell carcinoma (mRCC)

    DEFF Research Database (Denmark)

    Donskov, Frede; Marcussen, Niels; Hokland, M.

    2004-01-01

    -alpha-based immunotherapy in 34 patients and repeated after 5 weeks in 25 patients. Tumour proliferation was assessed by use of the anti-Ki-67 antibody MIB-1 and evaluated in multiple, random systematic sampled fields of vision. Ki-67 labelling index (LI) at baseline was median 13.6% (range 1.2-85.0) and median 10.......016). Baseline or change in Ki-67 LI did not correlate to survival. These data suggest that IFN-alpha in vivo has only modest effect on tumour proliferation in patients with mRCC. Tumour Ki-67 (MIB-1) reactivity after 1 month of immunotherapy appears to be a significant predictor of patient survival....

  10. Applied Ecotechnical Research on Lübao Slow-Release Water-Fertilizer-Nutrition Agent

    Institute of Scientific and Technical Information of China (English)

    黄大明; 张文奎; 沈子威; 席葆树

    2004-01-01

    bao slow-release water-fertilizer-nutrition agent (LSWA) is a new and proven highly effective soil additive. This nutrient absorbent polymer dramatically conserves water and reduces fertilizer usage while significantly increasing plant and crop yield. There are numerous benefits that LSWA is engineered to produce,and moreover, with every new application new benefits are discovered. The versatility of this additive will undoubtedly lead to some new applications. LSWA crystals in the soil absorb hundreds of times their mass in water and then, upon demand, release the stored moisture regardless of the soil condition. Unlike previously used polymers, LSWA has great absorbability even under soil pressure, and maintains its absorbability in the presence of fertilizers and other water soluble additives. It is completely non-toxic, and has a lengthy effective period of seven to ten years. LSWA is extremely cost effective due to its low initial cost, the dramatic reduction in the number of watering cycles, the decrease of fertilizer usage and the increase in crop yield, as well as allowing utilization of previously unusable land. When LSWA crystals are added into the soil, irrigation schedule times can be reduced by more than 50%, and fertilizer usage reduced by up to 33%. In this paper, some basic researches on the basic properties of the soil additive are reported. Some applications are described by reference to a number of case studies. Finally, specific recommendations are given for the application rates and methods for a range of crops and soil conditions.

  11. Constraints on Steep Equation of State for the Dark Energy using BAO

    CERN Document Server

    Jaber, Mariana

    2016-01-01

    We present a parametrization for the Dark Energy Equation of State "EoS" which has a rich structure. Our EoS has a transition at pivotal redshift $z_T$ between the present day value $w_0$ to an early time $w_i=w_a+w_0\\equiv w(z>>0)$ and the steepness of this transition is given in terms of the $q$ parameter. The proposed parametrization is $w=w_0+w_a(z/z_T)^q/(1+(z/z_T))^q$, with $w_0$, $w_i$, $q $ and $z_T$ constant parameters. This transition is motivated by scalar field dynamics such as for example quintessence models. Our parametrization reduces to the widely used EoS $w=w_0+w_a(1-a)$ for $z_T=q=1$. We study if a late time transition is favored by BAO measurements and Planck priors. According to our results, an EoS with a present value of $w_0 = -0.91$ and a high redshifts value $w_i =-0.62$, featuring a transition at a redshift of $z_T = 1.16$ with an exponent $q = 9.95$ is a good fit to the observational data. We found good agreement between the model and the data reported by the different surveys. A "t...

  12. Utility of tissue microarrays MIB-1 for profiling prognostic biomarkers in clinically localized prostate cancer Expressão imuno-histoquímica do MIB-1 como fator prognóstico em pacientes com adenocarcinoma de próstata localizado

    Directory of Open Access Journals (Sweden)

    Aissar Eduardo Nassif

    2009-12-01

    Full Text Available INTRODUCTION AND OBJECTIVE: To analyze the immuno-histochemical expression of MIB-1 marker as a prognostic factor in patients with localized prostate neoplasia. METHODS: Analysis of 100 cases of patients with localized prostate neoplasia who underwent curative surgery from January 2000 to December 2006. The customary histological preparation was carried out, followed by immunohistochemical reaction to detect MIB-1 protein accumulation and statistical analysis. RESULTS: There was a larger proportion of negative expression for MIB-1 marker (63.0%, p 10 ng/dl (p = 0.010, higher Gleason score (> 7 (p = 0.0003 and pathological stage pT2c (p = 0.001. In the comparison of variables, it was noticed that patients with positive expression had a higher mean PSA (p = 0.031, higher Gleason score (p INTRODUÇÃO E OBJETIVO: Analisar a expressão imuno-histoquímica do marcador MIB-1 como fator prognóstico em pacientes com neoplasia de próstata localizada. MÉTODOS: Análise de cem casos de pacientes portadores de neoplasia prostática localizada submetida a cirurgia curativa no período de janeiro de 2000 a dezembro de 2006. Realizou-se o preparo histológico habitual seguido de reação imuno-histoquímica para a detecção do acúmulo da proteína MIB-1 e análise estatística. RESULTADOS: Obteve-se maior proporção de expressão negativa ao marcador MIB-1(63%, p 10 ng/dl (p = 0,01, escore de Gleason mais alto (> 7 (p = 0,0003 e estádio patológico pT2c (p = 0,001. Na comparação das variáveis observou-se que os pacientes com expressão positiva apresentaram PSA médio mais elevado (p = 0,031, escore de Gleason mais alto (p < 0,0001, maior tempo de seguimento (p = 0,037, maior área de proliferação vascular (p < 0,0001 e maior proporção de tumor em relação à área normal (p = 0,012. CONCLUSÃO: O MIB-1 mostrou-se um fator prognóstico comparável a PSA, Gleason e estádio.

  13. Creatine kinase-MB: the journey to obsolescence.

    Science.gov (United States)

    Singh, Gurmukh; Baweja, Paramdeep S

    2014-03-01

    To evaluate the clinical utility of and demand for the creatine kinase (CK)-MB assay. We examined the number of CK-MB tests from 2007 through 2013 while we progressively deemphasized their use. We first removed CK-MB from the acute coronary syndrome (ACS) panel and then from the main menu and observed the demand for the test. We also reviewed patient medical records to assess the appropriateness of its use. After removing CK-MB from the ACS panel, the test volume dropped from around 12,000 per year to about 150 per year. In reviewing the records of 171 patients who had CK-MB determination done over a 28-month period, we discovered that CK-MB contributed to the diagnosis in only one patient, although it was not essential. Since removing CK-MB from the laboratory menu, two CK-MB tests were ordered in 4 months, and neither added value. CK-MB determinations do not add value to information available from the troponin assay and can be safely removed from the laboratory menu.

  14. A case study investigating the occurrence of geosmin and 2-methylisoborneol (MIB) in the surface waters of the Hinze Dam, Gold Coast, Australia.

    Science.gov (United States)

    Uwins, H K; Teasdale, P; Stratton, H

    2007-01-01

    The Hinze Dam is located in the Gold Coast hinterland and is the primary source of water supply for the Gold Coast region. Sporadic and unpredictable taste and odour events caused by geosmin and 2-methylisoborneol (MIB) are an ongoing problem in the Hinze Dam. To investigate potential ecological and physiological triggers of these events, a 12-month surface-sampling regime was undertaken. Concentrations of geosmin, MIB, nitrogen and phosphorus were measured. Algal and cyanobacterial counts were performed. Water temperature, rainfall and dam capacity were also recorded. The occurrence of geosmin was found to correlate significantly with Anabaena numbers, water temperature and dam capacity. The occurrence of MIB correlated with increasing ammonia. No significant correlations were observed with the other nutrients or physical parameters measured. Overall, this study demonstrated that high concentrations of geosmin detected in dam surface waters was strongly correlated with an increase in numbers of Anabaena spp. These events were most likely triggered by significant rainfall causing a pulse in nutrients into the dam, in conjunction with warmer water temperatures.

  15. Electronic structure, bulk and magnetic properties of MB{sub 6} and MB{sub 12} borides

    Energy Technology Data Exchange (ETDEWEB)

    Baranovskiy, A.E. [Institute for Low Temperature Physics and Engineering, NASU, Kharkov 61103 (Ukraine)]. E-mail: baranovskiy@ilt.kharkov.ua; Grechnev, G.E. [Institute for Low Temperature Physics and Engineering, NASU, Kharkov 61103 (Ukraine); Fil, V.D. [Institute for Low Temperature Physics and Engineering, NASU, Kharkov 61103 (Ukraine); Ignatova, T.V. [Institute for Low Temperature Physics and Engineering, NASU, Kharkov 61103 (Ukraine); Logosha, A.V. [Institute for Low Temperature Physics and Engineering, NASU, Kharkov 61103 (Ukraine); Panfilov, A.S. [Institute for Low Temperature Physics and Engineering, NASU, Kharkov 61103 (Ukraine); Svechkarev, I.V. [Institute for Low Temperature Physics and Engineering, NASU, Kharkov 61103 (Ukraine); Shitsevalova, N.Yu. [Institute for Problems of Materials Science, NASU, Kiev 03680 (Ukraine); Filippov, V.B. [Institute for Problems of Materials Science, NASU, Kiev 03680 (Ukraine); Eriksson, O. [Department of Physics, University of Uppsala, S-751 21 Uppsala (Sweden)

    2007-09-13

    The bulk and magnetic properties of MB{sub 6} and MB{sub 12} were investigated on the basis of first principles electronic structure calculations. The elastic constants were measured for ZrB{sub 12}, HoB{sub 12}, ErB{sub 12}, TmB{sub 12}, LuB{sub 12}, YB{sub 6} and LaB{sub 6} compounds at low temperatures. The calculated equations of states and balanced crystal orbital overlap populations have allowed to analyse bonding and magnetic properties of MB{sub 6} and MB{sub 12}.

  16. [Evaluation of the Creatine Kinase MB Activity Assay Kit].

    Science.gov (United States)

    Okubo, Manabu; Kimura, Shigeki; Matsui, Masahiko; Suehisa, Etsuji; Hidaka, Yoh

    2014-11-01

    CK-MB activity, which is measured by the immunoinhibition method, is an important marker for use in the diagnosis of acute coronary syndromes. In the present study, we evaluated the basal performance of a recently improved CK-MB activity kit, "L-system CK-MB," in which the activity of mitochondrial CK subunits is inhibited. Within-run and between-day precision were in the range of 1.9-2.3% and 3.2-6.0%, respectively. Diluted linearity and limit of detection were determined to be 600 U/L and 0.8 U/L, respectively. The use of L-system CK-MB allowed the inhibition of the activity of 98.1% of sarcomeric mitochondrial CK, 97.7% of ubiquitous mitochondrial CK, and 99.9% of CK-MM. The correlation coefficient (r) between CK-MB activity and CK-MB protein was 0.968. However, we found 4 cases showing CK-MB activity significantly higher than the protein concentration. Increased CK-BB activity was detected by electrophoresis in these cases. In some patients with malignant tumors, the presence of CK-immunoglobulin complex also lead to elevated CK-MB concentrations. Thus, the discrepancy in the CK-MB activity and the protein concentration may be caused by the presence of CK-BB and/or CK-immunoglobulin complex. More attention needs to be focused on samples with high CK-MB protein concentrations, especially when the CK-MB/CK ratio is high.

  17. Antidiarrheal properties of different extracts of Chinese herbal medicine formula Bao-Xie-Ning

    Institute of Scientific and Technical Information of China (English)

    Jing Li; Xin-lin Wu; Yuling Chen; Zhi Tang; Yue-hong Xu; Jian-min Jiang; Yue-yu Gu

    2013-01-01

    OBJECTIVE:Bao-Xie-Ning (BXN),a traditional Chinese herbal medicine (CHM) formula composed of Fructus Evodiae,Flos Caryophylli and Cortex Cinnamomi,and used for the treatment of infant diarrheal illness,was subject to systematic assessment for its putative multiple pharmacodynamic effects and pharmacological antidiarrheal mechanisms.METHODS:High-performance liquid chromatography-diode array detector-electrospray ionizationmass spectrometric/mass spectrometry was developed and validated for identification and quantification of the main constituents in different extracts of BXN.Male Kunming mice weighing 20 to 25 g were used for detecting the antidiarrheal activity of the extracts.Ethanolic extract (EE),volatile oil extract (VOE),and aqueous extract (AE) of BXN were respectively subjected to pharmacodynamic and pharmacological comparison in assessing antidiarrheal effects with senna-induced diarrhea,castor oil-induced diarrhea,acetic acid-induced writhing assay,and isolated duodenum test.RESULTS:The highest yields of three detected components of BXN,rutaecarpine,eugenol and cinnamaldehyde were observed in EE.EE showed the most remarkable antidiarrheal activity in dose-dependent and time-dependent manners in both senna-and castor oil-induced diarrhea models,and presented dose-dependent analgesic activity in acetic acid-induced algesthesia model.In addition,EE extract of BXN also exhibited strong antimobility action on the intestine and strongest depression on spontaneous contraction of isolated duodenum.CONCLUSION:Ethanol extraction is an efficient method to extract the active constituents of BXN.BXN extract demonstrated multiple pharmacological activities affecting the main mechanisms of diarrhea,which validated BXN's usage in the comprehensive clinical treatment of diarrhea.

  18. The clustering of galaxies in the completed SDSS-III Baryon Oscillation Spectroscopic Survey: tomographic BAO analysis of DR12 combined sample in Fourier space

    Science.gov (United States)

    Zhao, Gong-Bo; Wang, Yuting; Saito, Shun; Wang, Dandan; Ross, Ashley J.; Beutler, Florian; Grieb, Jan Niklas; Chuang, Chia-Hsun; Kitaura, Francisco-Shu; Rodriguez-Torres, Sergio; Percival, Will J.; Brownstein, Joel R.; Cuesta, Antonio J.; Eisenstein, Daniel J.; Gil-Marín, Héctor; Kneib, Jean-Paul; Nichol, Robert C.; Olmstead, Matthew D.; Prada, Francisco; Rossi, Graziano; Salazar-Albornoz, Salvador; Samushia, Lado; Sánchez, Ariel G.; Thomas, Daniel; Tinker, Jeremy L.; Tojeiro, Rita; Weinberg, David H.; Zhu, Fangzhou

    2017-04-01

    We perform a tomographic baryon acoustic oscillations (BAO) analysis using the monopole, quadrupole and hexadecapole of the redshift-space galaxy power spectrum measured from the pre-reconstructed combined galaxy sample of the completed Sloan Digital Sky Survey Baryon Oscillation Spectroscopic Survey (BOSS) Data Release12 covering the redshift range of 0.20 < z < 0.75. By allowing for overlap between neighbouring redshift slices, we successfully obtained the isotropic and anisotropic BAO distance measurements within nine redshift slices to a precision of 1.5-3.4 per cent for DV/rd, 1.8-4.2 per cent for DA/rd and 3.7-7.5 per cent for H rd, depending on effective redshifts. We provide our BAO measurement of DA/rd and H rd with the full covariance matrix, which can be used for cosmological implications. Our measurements are consistent with those presented in Alam et al., in which the BAO distances are measured at three effective redshifts. We constrain dark energy parameters using our measurements and find an improvement of the Figure-of-Merit of dark energy in general due to the temporal BAO information resolved. This paper is a part of a set that analyses the final galaxy clustering data set from BOSS.

  19. Global atmospheric circulation statistics, 1000-1 mb

    Science.gov (United States)

    Randel, William J.

    1992-01-01

    The atlas presents atmospheric general circulation statistics derived from twelve years (1979-90) of daily National Meteorological Center (NMC) operational geopotential height analyses; it is an update of a prior atlas using data over 1979-1986. These global analyses are available on pressure levels covering 1000-1 mb (approximately 0-50 km). The geopotential grids are a combined product of the Climate Analysis Center (which produces analyses over 70-1 mb) and operational NMC analyses (over 1000-100 mb). Balance horizontal winds and hydrostatic temperatures are derived from the geopotential fields.

  20. Avaliação do potencial de processos oxidativos avançados para remediação de águas contaminadas com geosmina e 2-MIB Evaluation of advanced oxidation processes for remediation of contamined water with geosmin and 2-MIB

    Directory of Open Access Journals (Sweden)

    Adriane Martins de Freitas

    2008-01-01

    Full Text Available In this work, the efficiency of some homogeneous advanced oxidation processes (UVC/H2O2, Fe2+/H2O2, UVC/Fe2+/H2O2, UVA/Fe2+/H2O2, solar/Fe2+/H2O2 was investigated toward the degradation of geosmin and 2-methylisoborneol (2-MIB. The effect of relevant experimental parameters (ie. pH, Fe2+ and H2O2 concentration was first investigated by factorial design, using camphor as a model substrate. In the geosmin and 2-MIB degradation studies the Fenton processes assisted by solar and UVA radiation offered the most promising results, mainly on account of high degradation capacity (higher than 80% at a reaction time of 60 min, high operational simplicity and low cost.

  1. New derivative of 2-(2,4-dihydroxyphenyl)thieno-1,3-thiazin-4-one (BChTT) elicits antiproliferative effect via p38-mediated cell cycle arrest in cancer cells.

    Science.gov (United States)

    Juszczak, Małgorzata; Walczak, Katarzyna; Matysiak, Joanna; Lemieszek, Marta K; Langner, Ewa; Karpińska, Monika M; Pożarowski, Piotr; Niewiadomy, Andrzej; Rzeski, Wojciech

    2016-03-15

    2-(2,4-Dihydroxyphenyl)thieno-1,3-thiazin-4-ones are a group of new compounds with potential anticancer activity. This type of derivatives was poorly investigated in the area of synthesis and biological activities. In the present study the antiproliferative action of the most active derivative BChTT was described. The aim of biological evaluation was to investigate the ability of the compound to inhibit cancer cell proliferation and identify mechanism involved in its action on the molecular level. BChTT inhibited the proliferation of lung cancer A549, colon cancer HT-29 and glioma C6 cells in the concentration-dependent manner. It was not toxic to normal cells including skin fibroblasts, hepatocytes and oligodendrocytes in the antiproliferative concentrations. BChTT decreased the DNA synthesis in the treated cancer cells and induced cell cycle arrest in the G0/G1 phase. Moreover, the ability of the compound to activate p38 kinase and decrease cyclin D1 expression was estimated. Participation of p38 kinase in the antiproliferative action of the compound was confirmed by the analysis of BChTT activity in the cells with the p38 silenced gene. The obtained results may suggest the ability of the tested derivative to inhibit cancer cells proliferation by induction of p38-mediated cyclin D1 downregulation.

  2. Antimicrobial Testing Methods & Procedures: MB-17-03

    Science.gov (United States)

    Information about ATMP - SOP Neutralization Confirmation Procedures for the AOAC Use-dilution method (UDM), the AOAC Germicidal Spray Products as Disinfectants Test (GSPT) and the Disinfectant Towelette Test (DTT) - MB-17-03

  3. Antimicrobial Testing Methods & Procedures: MB-32-00

    Science.gov (United States)

    Information about ATMP - SOP Quantitative Petri Plate Method (QPM) for Determining the Effectiveness of Antimicrobial Towelettes Against Spores of Clostridium difficile (ATCC 43598) on Inanimate, Hard Non-porous Surfaces - MB-32-00

  4. Antimicrobial Testing Methods & Procedures: MB-31-03

    Science.gov (United States)

    Information about ATMP - SOP Quantitative Disk Carrier Test Method (QCT-2) Modified for Testing Antimicrobial Products Against Spores of Clostridium difficile (ATCC 43598) on Inanimate, Hard, Non-porous Surfaces - MB-31-03

  5. Antimicrobial Testing Methods & Procedures: MB-26-01

    Science.gov (United States)

    Information about ATMP - SOP Neutralization of Microbicidal Activity using the OECD Quantitative Method for Evaluating Bactericidal Activity of Microbicides Used on Hard, Non-Porous Surfaces - MB-26-01

  6. Antimicrobial Testing Methods & Procedures: MB-33-00

    Science.gov (United States)

    Information about ATMP - SOP Quantitative Petri Plate Method (QPM) for Determining the Effectiveness of Antimicrobial Towelettes Against Vegetative Bacteria on Inanimate, Hard, Non-porous Surfaces - MB-33-00

  7. Interacting scalar tensor cosmology in light of SNeIa, CMB, BAO and OHD observational data sets

    Energy Technology Data Exchange (ETDEWEB)

    Rabiei, Sayed Wrya; Saaidi, Khaled [Faculty of Science University of Kurdistan, Department of Physics, Sanandaj (Iran, Islamic Republic of); Sheikhahmadi, Haidar [Faculty of Science University of Kurdistan, Department of Physics, Sanandaj (Iran, Islamic Republic of); Institute for Advance Studies in Basic Sciences (IASBS) Gava Zang, Zanjan (Iran, Islamic Republic of); Aghamohammadi, Ali [Sanandaj Branch Islamic Azad University, Sanandaj (Iran, Islamic Republic of)

    2016-02-15

    In this work, an interacting chameleon-like scalar field scenario, by considering SNeIa, CMB, BAO, and OHD data sets, is investigated. In fact, the investigation is realized by introducing an ansatz for the effective dark energy equation of state, which mimics the behavior of chameleon-like models. Based on this assumption, some cosmological parameters, including the Hubble, deceleration, and coincidence parameters, in such a mechanism are analyzed. It is realized that, to estimate the free parameters of a theoretical model, by regarding the systematic errors it is better that the whole of the above observational data sets would be considered. In fact, if one considers SNeIa, CMB, and BAO, but disregards OHD, it maybe leads to different results. Also, to get a better overlap between the contours with the constraint χ{sub m}{sup 2} ≤ 1, the χ{sub T}{sup 2} function could be re-weighted. The relative probability functions are plotted for marginalized likelihood L(Ω{sub m0}, ω{sub 1}, β) according to the two dimensional confidence levels 68.3, 90, and 95.4%. Meanwhile, the value of the free parameters which maximize the marginalized likelihoods using the above confidence levels are obtained. In addition, based on these calculations the minimum value of χ{sup 2} based on the free parameters of the ansatz for the effective dark energy equation of state is achieved. (orig.)

  8. In vivo assessment of the antiproliferative properties of interferon-alpha during immunotherapy: Ki-67 (MIB-1) in patients with metastatic renal cell carcinoma (mRCC)

    DEFF Research Database (Denmark)

    Donskov, Frede; Marcussen, Niels; Hokland, M.

    2004-01-01

    The aim of the present study was to investigate the in vivo antiproliferative effect of interferon alpha (IFN-alpha) in patients with metastatic renal cell carcinoma (mRCC). Core needle biopsies of metastatic and/or the primary kidney cancer were obtained before interleukin-2 (IL-2)- and IFN......-alpha-based immunotherapy in 34 patients and repeated after 5 weeks in 25 patients. Tumour proliferation was assessed by use of the anti-Ki-67 antibody MIB-1 and evaluated in multiple, random systematic sampled fields of vision. Ki-67 labelling index (LI) at baseline was median 13.6% (range 1.2-85.0) and median 10.......016). Baseline or change in Ki-67 LI did not correlate to survival. These data suggest that IFN-alpha in vivo has only modest effect on tumour proliferation in patients with mRCC. Tumour Ki-67 (MIB-1) reactivity after 1 month of immunotherapy appears to be a significant predictor of patient survival....

  9. The clustering of galaxies in the completed SDSS-III Baryon Oscillation Spectroscopic Survey: tomographic BAO analysis of DR12 combined sample in configuration space

    Science.gov (United States)

    Wang, Yuting; Zhao, Gong-Bo; Chuang, Chia-Hsun; Ross, Ashley J.; Percival, Will J.; Gil-Marín, Héctor; Cuesta, Antonio J.; Kitaura, Francisco-Shu; Rodriguez-Torres, Sergio; Brownstein, Joel R.; Eisenstein, Daniel J.; Ho, Shirley; Kneib, Jean-Paul; Olmstead, Matthew D.; Prada, Francisco; Rossi, Graziano; Sánchez, Ariel G.; Salazar-Albornoz, Salvador; Thomas, Daniel; Tinker, Jeremy; Tojeiro, Rita; Vargas-Magaña, Mariana; Zhu, Fangzhou

    2017-08-01

    We perform a tomographic baryon acoustic oscillations (BAOs) analysis using the two-point galaxy correlation function measured from the combined sample of Baryon Oscillation Spectroscopic Survey Data Release 12 (BOSS DR12), which covers the redshift range of 0.2 clustering, we obtain a measurement of DA(z)/rd and H(z)rd at nine effective redshifts with the full covariance matrix calibrated using MultiDark-Patchy mock catalogues. Using the reconstructed galaxy catalogues, we obtain the precision of 1.3-2.2 per cent for DA(z)/rd and 2.1-6.0 per cent for H(z)rd. To quantify the gain from the tomographic information, we compare the constraints on the cosmological parameters using our nine-bin BAO measurements, the consensus three-bin BAO and redshift space distortion measurements at three effective redshifts in Alam et al., and the non-tomographic (one-bin) BAO measurement at a single effective redshift. Comparing the nine-bin with one-bin constraint result, it can improve the dark energy Figure of Merit by a factor of 1.24 for the Chevallier-Polarski-Linder parametrization for equation-of-state parameter wDE. The errors of w0 and wa from nine-bin constraints are slightly improved when compared to the three-bin constraint result.

  10. The clustering of galaxies in the completed SDSS-III Baryon Oscillation Spectroscopic Survey: tomographic BAO analysis of DR12 combined sample in Fourier space

    CERN Document Server

    Zhao, Gong-Bo; Saito, Shun; Wang, Dandan; Ross, Ashley J; Beutler, Florian; Grieb, Jan Niklas; Chuang, Chia-Hsun; Kitaura, Francisco-Shu; Rodriguez-Torres, Sergio; Percival, Will J; Brownstein, Joel R; Cuesta, Antonio J; Eisenstein, Daniel J; Gil-Marín, Héctor; Kneib, Jean-Paul; Nichol, Robert C; Olmstead, Matthew D; Prada, Francisco; Rossi, Graziano; Salazar-Albornoz, Salvador; Samushia, Lado; Sánchez, Ariel G; Thomas, Daniel; Tinker, Jeremy L; Tojeiro, Rita; Weinberg, David H; Zhu, Fangzhou

    2016-01-01

    We perform a tomographic baryon acoustic oscillations (BAO) analysis using the monopole, quadrupole and hexadecapole of the redshift-space galaxy power spectrum measured from the pre-reconstructed combined galaxy sample of the completed Sloan Digital Sky Survey (SDSS-III) Baryon Oscillation Spectroscopic Survey (BOSS) Data Release (DR)12 covering the redshift range of $0.20BAO distance measurements within nine redshift slices to a precision of $1.5\\%-3.4\\%$ for $D_V/r_d$, $1.8\\% -4.2\\%$ for $D_A/r_d$ and $3.7\\% - 7.5\\%$ for $H \\ r_d$, depending on effective redshifts. We provide our BAO measurement of $D_A/r_d$ and $H \\ r_d$ with the full covariance matrix, which can be used for cosmological implications. Our measurements are consistent with those presented in \\citet{Acacia}, in which the BAO distances are measured at three effective redshifts. We constrain dark energy parameter...

  11. THEORETICAL DEPENDENCE OF LONG WAVELENGTH PHOTOEMISSION UPON THE SIZE OF Ag NANOPARTICLES EMBEDDED IN BaO SEMICONDUCTOR THIN FILM

    Institute of Scientific and Technical Information of China (English)

    杨海; 蔡武德; 许北雪; 吴锦雷

    2001-01-01

    The dependence of long wavelength photoemission upon the size of Ag nanoparticles embedded in a BaO semicon- ductor is predicted and discussed theoretically. The calculated results show that the increase in the diameter of the Ag nanoparticle, in the range from 1.5 to 37.0nm, leads to the emergence of a roughly Gaussian form of the photoemission spectra and the peaks become markedly narrower. The results also show that the increase in the diameter of the Agnanoparticle leads to the decrease of the long wavelength threshold. The incident light wavelength corresponding to the peak value of the photoemission gets bigger with the increase of the size of Ag nanoparticles, thus showing a redshift.

  12. Mining Gold in Africa——Development Course of Bao Yao Huan Jian Iron Steel Group Ltd.

    Institute of Scientific and Technical Information of China (English)

    Bai Yifeng

    2006-01-01

    @@ Historical Trail Founded in 1998,the company jointly ventured with a Nigerian company and invested US$1 million to establish a small rolling mill.In the beginning of 2000,the company established Bao Yao Huan Jian Iron& Steel Group Ltd.with an independent investment of US$15 million with the standard workshop covering an area of 38000,office and logistics workshop occupying an area of 4,000 m2.The group can produce over 300,000 tons of various iron & steel rolling steel materials annually.In 2003,the group set up more companies such as Bulconexim International Ltd,BYHJ shipping Co.,Ltd.and Construction Co.,Ltd.one after another.In 2005,additional fund of US$4 million was invested to set up MAWA Textile Nigeria FZE,the workshop of which has the ability to produce 5 million meters of all kinds of textile each year.

  13. CARDIOPROTECTIVE ROLES OF THE CHINESE MEDICINAL FORMULA BAO-XIN-TANG ON ACUTE MYOCARDIAL INFARCTION IN RATS.

    Science.gov (United States)

    Wang, Yang; Wang, Weihao; Peng, Weijun; Liu, Weiping; Cai, Weijun; Xia, Zian; Zhang, Honggeng; Xing, And Zhihua

    2017-01-01

    Bao-Xin-Tang (BXT) is a traditional Chinese medicinal formula used for the treatment of coronary heart disease and known to have favorable therapeutic benefits. The current study was designed to determine whether BXT has a cardioprotective role for acute myocardial infarction. The underlying mechanisms were also explored. The Sprague-Dawley rat model of acute myocardial infarction was established by occluding the left anterior descending branch of the coronary artery. After a 3-h ischemic period, we determined the myocardial infarction size, inflammatory components, and antioxidant activities. The data showed that BXT could reduce the infarction size and lower the levels of C-reactive protein, interleukin-6, and myeloperoxidase, and increase the activities of superoxide dismutase and the anti-inflammatory cytokine, interleukin-10. These results indicate that administration of BXT, following acute myocardial infarction, could reduce infarct size. The effects of BXT may be related to its anti-inflammatory and anti-oxidative properties.

  14. Creatine kinase MB isoenzyme in dermatomyositis: a noncardiac source

    Energy Technology Data Exchange (ETDEWEB)

    Larca, L.J.; Coppola, J.T.; Honig, S.

    1981-03-01

    Three patients with polymyositis had elevated serum levels of creatine kinase MB isoenzyme. The presence of this isoenzyme is used extensively to diagnose myocardial infarction, but the isoenzyme is also found in sera of patients with primary muscular and neuromuscular disorders. Researchers studied cardiac function in two of our patients with electrocardiograms, technetium stannous pyrophosphate scanning, and technetium 99m-labeled erythrocyte gated blood pool imaging and in the third patient by postmortem examination. There was no evidence of myocardial involvement to account for the high serum levels of isoenzyme. Creatine kinase MB in the sera of patients with polymyositis does not necessarily indicate myocardial necrosis.

  15. Neurotransmitters in the Gas Phase: La-Mb Studies

    Science.gov (United States)

    Cabezas, C.; Mata, S.; López, J. C.; Alonso, J. L.

    2011-06-01

    LA-MB-FTMW spectroscopy combines laser ablation with Fourier transform microwave spectroscopy in supersonic jets overcoming the problems of thermal decomposition associated with conventional heating methods. We present here the results on LA-MB-FTMW studies of some neurotransmitters. Six conformers of dopamine, four of adrenaline, five of noradrenaline and three conformers of serotonin have been characterized in the gas phase. The rotational and nuclear quadrupole coupling constants extracted from the analysis of the rotational spectrum are directly compared with those predicted by ab initio methods to achieve the conclusive identification of different conformers and the experimental characterization of the intramolecular forces at play which control conformational preferences.

  16. Modem Handles Data At 160 Mb/s

    Science.gov (United States)

    Budinger, James M.; Miller, Susan P.; Kappes, J. Mark; Layer, David; House, Gilmore

    1993-01-01

    Trellis-coded octal-phase-shift-keying modem transmits and receives continuous or bursted digital data for satellite applications. Helps satisfy increasing demands for communication of high-speed digital data from sources like high-performance computers and high-definition television transmitters, as well as multiplexed signals from slower signal sources. Increases maximum effective bit rate accommodated in standard 72-MHz-wide commercial satellite-transponder radio channel from 120 Mb/s to 160 Mb/s. Principal advantages: spectral efficiency and power efficiency.

  17. Function and regulation of cholecystokinin octapeptide, β-endorphin and gastrin in anorexic infantile rats treated with ErBao Granules

    Institute of Scientific and Technical Information of China (English)

    Yong Ping Du; Yue Ping Zhang; Shou Chuan Wang; Jian Shi; Shao Hua Wu

    2001-01-01

    AIM To study the role of cholecystokinin octapeptide ( CCK-8), β-endorphin ( β-EP), and gastrin in an anorexic infantile rat model and no subsequent regulation of nose peptides by the Yunpi complex prescription ErBao Granule. METHODS We fed infantile rats with special prepared forage. A liquid extract of ErBao Granule was administered to the rats daily for 3weeks, CCK-8, β-EP, and gastrin concentrations in hypothalamus, gastric antrum, and plasma of the rats were measured by radioimmunoassay,and were compared with controls. RESULTS Treatment of rats with ErBao Granule inhibited CCK-8 secretion and increased β-EP and gastrin secretion. CCK-8 concentration in hypothalamus and plasma of model control group increased significantly and correlated negatively with food intake of models.respectively. β-EP concentration in gastric antrum and plasma of model control group decreased significantly and showed a positive correlation with food intake of models,respectively. Hypothalamus concentration of β-EP was similar in models and controls. Gastrin concentration in gastric antrum of models was lower than in the blank control group, and correlated positively to food intake of models.Finally, CCK-8 concentrations in plasma of rats showed a positive correlation with plasma β-EP(r- 0.68, P<0.05).CONCLUSION The increased plasma and hypothalamus concentration of CCK-8, decreased gastric antrum and plasma level of β-EP. and decreased gastric antrum concentration of gastrin are associated significantly with the anorexia of infantile anorexic rat models produced by special forage. ErBao Granule can reverse these changes, which may be the major mechanisms of ErBao Granule simulating feeding.

  18. Value of tissue markers p27kip1, MIB-1, and CD44s for the pre-operative prediction of tumour features in screen-detected prostate cancer

    NARCIS (Netherlands)

    A.N. Vis (André); B.W. van Rhijn (Bas); M.A. Noordzij (Marinus); F.H. Schröder (Fritz); Th.H. van der Kwast (Theo)

    2002-01-01

    textabstractThe pre-operative prediction of prognostic tumour features in the radical prostatectomy specimen using routine clinicopathological variables remains limited. The present study evaluated the predictive value of the cell-cycle protein p27kip1, the proliferation marker MIB-1, and the cell-a

  19. Batch and continuous biodegradation of Amaranth in plain distilled water by P. aeruginosa BCH and toxicological scrutiny using oxidative stress studies.

    Science.gov (United States)

    Jadhav, Shekhar B; Patil, Nilambari S; Watharkar, Anuprita D; Apine, Onkar A; Jadhav, Jyoti P

    2013-05-01

    Bacterium Pseudomonas aeruginosa BCH was able to degrade naphthylaminesulfonic azo dye Amaranth in plain distilled water within 6 h at 50 mg l(-1) dye concentration. Studies were carried out to find the optimum physical conditions and which came out to be pH 7 and temperature 30 °C. Amaranth could also be decolorized at concentration 500 mg l(-1). Presence of Zn and Hg ions could strongly slow down the decolorization process, whereas decolorization progressed rapidly in presence of Mn. Decolorization rate was increased with increasing cell mass. Induction in intracellular and extracellular activities of tyrosinase and NADH-DCIP reductase along with intracellular laccase and veratryl alcohol oxidase indicated their co-ordinate action during dye biodegradation. Up-flow bioreactor studies with alginate immobilized cells proved the capability of strain to degrade Amaranth in continuous process at 20 ml h(-1) flow rate. Various analytical studies viz.--HPLC, HPTLC, and FTIR gave the confirmation that decolorization was due to biodegradation. From GC-MS analysis, various metabolites were detected, and possible degradation pathway was predicted. Toxicity studies carried out with Allium cepa L. through the assessment of various antioxidant enzymes viz. sulphur oxide dismutase, guaiacol peroxidase, and catalase along with estimation of lipid peroxidation and protein oxidation levels conclusively demonstrated that oxidative stress was generated by Amaranth.

  20. Carotenoid biosynthesis in bacteria: In vitro studies of a crt/bch transcription factor from Rhodobacter capsulatus and carotenoid enzymes from Erwinia herbicola

    Energy Technology Data Exchange (ETDEWEB)

    O' Brien, D.A.

    1992-11-01

    A putative transcription factor in Rhodobactor capsulatus which binds upstream of the crt and bch pigment biosynthesis operons and appears to play a role in the adaptation of the organism from the aerobic to the anaerobic-photosynthetic growth mode was characterized. Chapter 2 describes the identification of this factor through an in vitro mobility shift assay, as well as the determination of its binding properties and sequence specificity. Chapter 3 focuses on the isolation of this factor. Biochemistry of later carotenoid biosynthesis enzymes derived from the non-photosynthetic bacterium, Erwinia herbicola. Chapter 4 describes the separate overexpression and in vitro analysis of two enzymes involved in the main sequence of the carotenoid biosynthesis pathway, lycopene cyclase and 5-carotene hydroxylase. Chapter 5 examines the overexpression and enzymology of functionally active zeaxanthin glucosyltransferase, an enzyme which carries out a more unusual transformation, converting a carotenoid into its more hydrophilic mono- and diglucoside derivatives. In addition, amino acid homology with other glucosyltransferases suggests a putative binding site for the UDP-activated glucose substrate.

  1. Carotenoid biosynthesis in bacteria: In vitro studies of a crt/bch transcription factor from Rhodobacter capsulatus and carotenoid enzymes from Erwinia herbicola

    Energy Technology Data Exchange (ETDEWEB)

    O`Brien, David Allen [Univ. of California, Berkeley, CA (United States)

    1992-11-01

    A putative transcription factor in Rhodobactor capsulatus which binds upstream of the crt and bch pigment biosynthesis operons and appears to play a role in the adaptation of the organism from the aerobic to the anaerobic-photosynthetic growth mode was characterized. Chapter 2 describes the identification of this factor through an in vitro mobility shift assay, as well as the determination of its binding properties and sequence specificity. Chapter 3 focuses on the isolation of this factor. Biochemistry of later carotenoid biosynthesis enzymes derived from the non-photosynthetic bacterium, Erwinia herbicola. Chapter 4 describes the separate overexpression and in vitro analysis of two enzymes involved in the main sequence of the carotenoid biosynthesis pathway, lycopene cyclase and 5-carotene hydroxylase. Chapter 5 examines the overexpression and enzymology of functionally active zeaxanthin glucosyltransferase, an enzyme which carries out a more unusual transformation, converting a carotenoid into its more hydrophilic mono- and diglucoside derivatives. In addition, amino acid homology with other glucosyltransferases suggests a putative binding site for the UDP-activated glucose substrate.

  2. Evaluation of 1p and 14q status, MIB-1 labeling index and progesterone receptor immunoexpression in meningiomas: Adjuncts to histopathological grading and predictors of aggressive behavior

    Directory of Open Access Journals (Sweden)

    Sawan Kumar

    2014-01-01

    Full Text Available Background: Meningiomas are benign central nervous system tumors; however, significant fraction recurs, irrespective of histological grade. Materials and Methods: We performed fluorescence in-situ hybridization for 1p36 and 14q32, and immunohistochemistry for progesterone receptor (PR, p53 and MIB-1 on 84 meningiomas. Results and Conclusion: Sixty-four were convexity tumors (30 grade I, 21 grade II, 13 grade III and 20 petroclival (grade I; 10 with gross total resection (GTR, 10 with subtotal resection (STR. Isolated 1p36 deletion was seen in 20% grade I, 28.6% grade II and 30.8% grade III convexity meningiomas, and isolated 14q deletion in one grade III convexity tumor. 1p/14q co-deletion was seen in none of grade I, 28.5% grade II and 30% grade III convexity meningiomas. PR immunoreactivity was less frequent in grade III tumors. Petroclival tumors did not show isolated deletion. However, 1p/14q co-deletion was seen in 20% of petroclival tumors with STR and in none with GTR. Frequency of chromosomal alterations and MIB-1 labeling index both increase with tumor grade. 1p/14q co-deletion is characteristic of grade II/III meningiomas, while PR immunoreactivity inversely correlates with grade, suggesting their use as surrogate markers for grading. Identification of 1p/14q co-deletion in grade I petroclival tumors with STR suggests that unresectable petroclival meningiomas are biologically more aggressive than their grade I convexity counterparts.

  3. MB39C326:DC/DC转换器

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    富士通半导体(上海)有限公司推出面向便携设备的DC/DC转换器MB39C326,可通过自动切换降压,升压工作模式来扩大工作电压范围。MB39C326通过内置开关FET和采用2.15mm×1.94mm的小型封装构成贴装面积小、物料清单成本低的电源系统,且可通过DAC信号动态控制输出电压,支持APT、ET功能。

  4. SAW chirp Fourier transform for MB-OFDM UWB receiver

    Institute of Scientific and Technical Information of China (English)

    HE Peng-fei; L(U) Ying-hua; ZHANG Hong-xin; WANG Ye-qiu; XU Yong

    2006-01-01

    In the conventional multiband orthogonal frequency division multiplexing ultra wideband (MB-OFDM UWB )receiver, the fast Fourier transform (FFT) algorithm is realized by the expensive and power-consuming digital signal processor (DSP) chips. In this article, the lower power, lower cost, and lower complexity real-time analog surface acoustic wave (SAW)chirp Fourier transform devices were used to replace the DSP part. A MB-OFDM UWB receiver based on the M-C-M SAW chirp Fourier transform was presented, and the step of signal transformation from input signals was also depicted. The simulation results show that the proposed receiver provides similar bit error performance compared to the fully digital receiver when used in the channel environments proposed by the IEEE 802.15SG3a.

  5. Arbutin in Serratula quinquefolia M.B. (Asteraceae)

    OpenAIRE

    Gerard Nowak; Joanna Nawrot; Karol Latowski

    2011-01-01

    Genus Serratula is known for the presence of steroid compounds which belong to the group of ecdysones. Former phytochemical works from the late sixties and early seventies indicate, in some Serratula species, the occurrence of a phenolic glycoside-arbutin. This has been confirmed in the present work through finding an α,β-arbutin anomer in Serratula quinquefolia M.B. New botanical data concerning the classification of genus Serratula suggest that the species in question should be regarded as ...

  6. Melt Rate Improvement for DWPF MB3: Summary and Recommendations

    Energy Technology Data Exchange (ETDEWEB)

    Lambert, D.P.

    2001-07-11

    The objective for this task is to understand and apply the control of glass batch chemistry (frit composition) and/or changes in chemical processing strategies to improve the overall melting process for Macrobatch 3 (MB3) (Defense Waste Processing Facility (DWPF) sludge-only processing). For melt rate limited systems, a small increase in melting efficiency translates into substantial savings by reducing operational costs without compromising the quality of the final waste product. This report summarizes the key information collected during the FY01 melt-rate testing completed to support the conclusion that switching from Frit 200, the frit currently used to prepare all the glass produced in radioactive processing, to Frit 320 should improve the melt rate during processing of DWPF MB3 sludge (Note: MB3 is referred to as Sludge Batch 2 in the High-Level Waste System Plan). The report also includes recommendations that should be addressed prior to implementation of the new frit and future research that should be completed to further improve melt rate. No analysis has been completed to determine if Frit 320 can be used in processing of other sludge macrobatches. The testing in this report is based on dried-slurry testing of a MB3 melter feed prepared from nonradioactive simulants. Additional testing, particularly with a melter feed slurry and actual waste, would be required before implementing the new frit in DWPF, and a variability study would also be necessary. The work to date, at most, provides relative data until actual melter data can be obtained and compared.

  7. Study on borate glass system containing with Bi 2O 3 and BaO for gamma-rays shielding materials: Comparison with PbO

    Science.gov (United States)

    Kaewkhao, J.; Pokaipisit, A.; Limsuwan, P.

    2010-04-01

    In this work, the mass attenuation coefficients and shielding parameters of borate glass matrices containing with Bi 2O 3 and BaO have been investigated at 662 keV, and compare with PbO in same glass structure. The theoretical values were calculated by WinXCom software and compare with experiential data. The results found that the mass attenuation coefficients were increased with increasing of Bi 2O 3, BaO and PbO concentration, due to increase photoelectric absorption of all glass samples. However, Compton scattering gives dominant contribution to the total mass attenuation coefficients for studied glass samples. Moreover the half value layers (HVL) of glass samples were also better than ordinary concretes and commercial window glass. These results reflecting that the Bi-based glass can use replace Pb in radiation shielding glass. In the case of Ba, may be can use at appropriate energy such as X-rays or lower.

  8. Diffuse Reflectance Infrared Fourier Transform Study of NOx Adsorption on CGO10 Impregnated with K2O or BaO

    DEFF Research Database (Denmark)

    Traulsen, Marie Lund; Härelind Ingelsten, H.; Kammer Hansen, Kent

    2012-01-01

    In the present work Diffuse Reflectance Infrared Fourier Transform (DRIFT) spectroscopy is applied to study the adsorption of NOx at 300-500 °C in different atmospheres on gadolinium doped ceria (CGO), an important material in electrodes investigated for electrochemical NOx removal. Furthermore......, the effect on the NOx adsorption when adding K2O or BaO to the CGO is investigated. The DRIFT study shows mainly the presence of nitrate species at 500 °C, while at lower temperature a diversity of adsorbed NOx species exists on the CGO. Presence of O2 is shown to have a strong effect on the adsorption of NO......, but no effect on the adsorption of NO2. Addition of K2O and BaO dramatically affects the NOx adsorption and the results also show that the adsorbed NOx species are mobile and capable of changing adsorption state in the investigated temperature range....

  9. 试论鲍照悲剧意识的形成原因%Reasons for Bao Zhao's Tragedy Consciousness

    Institute of Scientific and Technical Information of China (English)

    郑国周

    2011-01-01

    From the existing poems, the poet Bao Zhao has a strong sense of tragedy. Investigating the reasons, three planes we can discuss at least. Perspective from personal circumstances, Bao Zhao was born in a poor family, and life experience was bad, valetudinarianism, suffering from pains, and feeling more difficult to reach the ideal expectations. From system reality perspectives, the family system, political struggle heavily sinister horrifying, cultural autocratic intensifies, plus reality, made Bao Zhao at loose ends. From cultural trend perspectives, the traditional tragic life experience and aesthetic psychology of sadness as beauty in the Han and Wei Dynasties influenced Bao Zhao deeply.%从现存诗文看,诗人鲍照有强烈的悲剧意识。究其形成原因,至少有三个层面可以讨论。从个人境遇层面看,鲍照家境贫寒,身世不旺,体弱多病,饱受病痛折磨,且倍感理想难达,事与愿违;从制度现实层面看,门阀制度森严,政治斗争险恶惨烈,文化专制加剧,加上现实龌龊不公,让鲍照无所适从;从文化思潮层面看,传统悲剧性生命体验及汉魏以来以悲为美的审美心理对鲍照影响很深。

  10. A quality control research of Bao tai pills%穿黄消炎胶囊的质量控制研究

    Institute of Scientific and Technical Information of China (English)

    陶善斌; 汪电雷

    2013-01-01

    Objective To establish the method for the quality control research of Bao tai pills. Methods TLC was used to make a thin layer qualitative identification for Herba Andrographis and Soliclago decur in Bao tai pills. Determination content of Paeoniflorin in Bao tai pills was conducted by HPLC. Results The TLC identification chromatography showed obvious characteristics. Paeoniflorin had good linear relationship in the concentration range of 12. 92 ~ 129. 20 mg · L-1( r = 0. 9997 ). The average recovery was 99. 52% ,and RSD was 0. 66% . Conclusion The method is accurate,delicate and reproducible,which can be applied for the quality control research of Bao tai pills.%目的 建立穿黄消炎胶囊的质量控制研究方法.方法采用薄层色谱法对穿黄消炎胶囊中的穿心莲和一只黄花进行定性鉴别;采用高效液相色谱测定脱水穿心莲内酯含量.结果 薄层鉴别色谱特征明显,专属性强;脱水穿心莲内酯在12.92~129.20 mg·L-1范围内线性关系良好(r=0.999 7),平均加样回收率为99.52%(RSD=0.66%).结论该方法准确、灵敏,重复性好,可作为穿黄消炎胶囊的质量控制研究.

  11. Electrochemical reduction of NO with propene in the presence of oxygen on LSCoM/CGO porous cell stacks impregnated with BaO

    DEFF Research Database (Denmark)

    Friedberg, Anja Zarah; Kammer Hansen, Kent

    2015-01-01

    The electrochemical reduction of NO with propene in the presence of 10 % O2 was studied on a La0.85Sr0.15Co0.03Mn0.97O3-Ce0.9Gd0.1O1.95 11-layer electrochemical reactor. BaO was impregnated into the porous reactor, and electrochemical impedance spectroscopy was used for characterisation in the te...

  12. Effect of Strong Basic Oxide (Li2O, Na2O, K2O and BaO) on Property of CaO-Based Flux

    Institute of Scientific and Technical Information of China (English)

    LI Gui-rong

    2003-01-01

    It is found that strong basic oxides including Li2O, Na2O, K2O and BaO, which are used to replace a part of CaO in CaO-based fluxes, can lower the melting point and the viscosity and enhance the dephosphorizing ability. The mechanism was analysed and the addition of Li2O to CaO-based fluxes was recommended.

  13. The Tao of bao: a randomised controlled trial examining the effect of steamed bun consumption on night-call inpatient course and mortality.

    Science.gov (United States)

    Tan, Min-Han; Lee, Ziying; Ng, Beatrice; Sim, Eng Swen; Chua, Ying Ying; Tien, Mark; Ooi, Choon Jin

    2008-03-01

    Medical superstitions remain prevalent in today's stressful and technology driven healthcare environment. These irrational beliefs commonly involve night calls, which are periods of volatile workload. In Singapore and Hong Kong, it is commonly held that consumption of steamed buns ("bao") by on-call physicians is associated with increased patient admissions and mortality, due to a homonymous interpretation of the word "bao" in dialect. A prospective unblinded randomised controlled trial with a permuted block randomisation design was performed on weekdays over 6 weeks. Steamed buns or control food were offered to the internal medicine night-call team of a tertiary-care hospital on a nightly basis. Information on admissions and mortality was collected from the hospital electronic database. Data on sleep patterns and shift duration were obtained by interview. There were no significant differences in the median number of hours slept on days on "bao" administration versus "control" intervention (2 +/- median absolute variation of 1.5 h vs 2 +/- 1.5 h, P = 0.30) or in the number of hours spent in the hospital (30.8 +/- 1.9 h vs 30.5 +/- 2.2 h, P = 0.09). There were no significant differences in the median number of general ward admissions per night (n = 73 +/- 6 versus 71 +/- 7 admissions, P = 0.35), monitored care unit admissions (4 +/- 1.5 vs 4 +/- 1.5 admissions, P = 0.65) or inpatient mortality (2 +/- 1.5 vs 2 +/- 1.5 deaths per night, P = 0.47). The consumption of steamed buns ("bao") has no effect on inpatient admissions, mortality, or sleep duration on call. Regardless, our results indicate that the night call in Singapore remains a challenge in terms of workload and shift duration.

  14. 120 MB/S and 240 MB/S bit synchronizer-signal conditioners for NASA high data rate applications

    Science.gov (United States)

    Gray, J. S.

    1976-01-01

    Two bit synchronizer-signal conditioners (BSSC) developed for NASA high data rate applications such as earth resources monitoring are described. One BSSC is centered at 120 MB/s and the other at 240 Mb/s. These subsystems are featured out of the total hardware developed because the BSSC is such a key subsystem in determining overall system statistical performance. These units represent an evolution of high data rate BSSC's available at low data rates. Numerous inputs/outputs, control functions, indicators, plus the ability to minimize the effects of various signal perturbations are provided. Examples of allowed perturbations are input level variations, bit rate variance static and dynamic, baseline, transition density, bandlimiting, etc., as well as noise. Emphasis in the past has been primarily concerned only with noise.

  15. The clustering of galaxies in the completed SDSS-III Baryon Oscillation Spectroscopic Survey: tomographic BAO analysis of DR12 combined sample in configuration space

    CERN Document Server

    Wang, Yuting; Chuang, Chia-Hsun; Ross, Ashley J; Percival, Will J; Gil-Marín, Héctor; Cuesta, Antonio J; Kitaura, Francisco-Shu; Rodriguez-Torres, Sergio; Brownstein, Joel R; Eisenstein, Daniel J; Ho, Shirley; Kneib, Jean-Paul; Olmstead, Matt; Prada, Francisco; Rossi, Graziano; Sánchez, Ariel G; Salazar-Albornoz, Salvador; Thomas, Daniel; Tinker, Jeremy; Tojeiro, Rita; Vargas-Magaña, Mariana; Zhu, Fangzhou

    2016-01-01

    We perform a tomographic baryon acoustic oscillations analysis using the two-point galaxy correlation function measured from the combined sample of BOSS DR12, which covers the redshift range of $0.2BAO measurements, the consensus 3-bin BAO and RSD measurements at three effective redshifts in \\citet{Alam2016}, and the non-tomographic (1-bin) BAO measurement at a single effective redshift. Comparing the 9-bin with 1-bin constraint result, it can improve the...

  16. The Semi-Quantitative Study of Magnetization Process on Milling and Reannealing of Barium Hexaferrite (BaO.6Fe2O3

    Directory of Open Access Journals (Sweden)

    Ridwan

    2009-07-01

    Full Text Available Barium hexaferrite (BaO.6Fe2O3 is as a hard magnetic material with good chemical stability which has been intensively used as permanent magnet components. Many works have been done in order to improve their magnetic properties either through chemical process or powders metallurgy technique. In this work, commercial BaO.6Fe2O3 was milled using high-energy milling machine for 10, 20 and 30 hours and followed by reannealing for 3 hours at 1000⁰C in air. X-ray diffraction pattern indicate no phase decomposition occurred caused the mill processing, annealing of milled powders recovered the crystal system and promoted crystallite growth. The magnetic hysteresis curve measured by vibrating sample magnetometer (VSM shows the coercivity of annealed BaO.6Fe2O3 increases to two times higher than the original one. By using Jiles-Atherton model, all the hysteresis parameters Ms, k, α, a and c, have been determined adopted to the Genetic Algorithm (GA. The analyzed hysteretic parameters obtained from this work is congruent to the change of magnetic properties of as-milled and annealed powders of barium hexaferrite.

  17. Biogeochemical characteristics of Kuan-Tzu-Ling, Chung-Lun and Bao-Lai hot springs in southern Taiwan.

    Science.gov (United States)

    Maity, Jyoti Prakash; Liu, Chia-Chuan; Nath, Bibhash; Bundschuh, Jochen; Kar, Sandeep; Jean, Jiin-Shuh; Bhattacharya, Prosun; Liu, Jiann-Hong; Atla, Shashi B; Chen, Chien-Yen

    2011-01-01

    Hot springs are the important natural sources of geothermally heated groundwater from the Earth's crust. Kuan-Tzu-Ling (KTL), Chung-Lun (CL) and Bao-Lai (BL) are well-known hot springs in southern Taiwan. Fluid and mud (sediments) samples were collected from the eruption points of three hot springs for detailed biogeochemical characterization. The fluid sample displays relatively high concentrations of Na(+) and Cl(-) compared with K(+), Mg(2+), Ca(2+), NO(2) (-), and SO(4) (2-), suggesting a possible marine origin. The concentrations of Fe, Cr, Mn, Ni, V and Zn were significantly higher in the mud sediments compared with fluids, whereas high concentrations of As, Ba, Cu, Se, Sr and Rb were observed in the fluids. This suggests that electronegative elements were released during sediment-water interactions. High As concentration in the fluids was observed to be associated with low redox (Eh) conditions. The FTIR spectra of the humic acid fractions of the sediments showed the presence of possible functional groups of secondary amines, ureas, urethanesm (amide), and silicon. The sulfate-reducing deltaproteobacterium 99% similar to Desulfovibrio psychrotolerans (GU329907) were rich in the CL hot spring while mesophilic, proteolytic, thiosulfate- and sulfur-reducing bacterium that 99% similar to Clostridium sulfidigenes (GU329908) were rich in the BL hot spring.

  18. Ultrathin oxide films and heterojunctions: CaO layers on BaO and SrO.

    Science.gov (United States)

    Mohn, Chris E; Allan, Neil L; Harding, John H

    2009-05-07

    We examine the form of the islands formed by CaO on BaO and SrO substrates using both periodic density functional theory and atomistic simulation techniques. (100) edges dominate the island morphology and we examine how the CaO adjusts to the substrate in small and medium sized islands and at much larger coverages. There is no direct overlay of CaO ion pairs over OBa or OSr pairs in the top substrate layer. Rather, island bond lengths are all much shorter than those even in bulk CaO, even in the interior of the islands, and more similar to those in CaO clusters and isolated thin films. Corner atoms are associated with particularly short Ca-O bond lengths and the low coordination numbers at such positions. The islands show a marked deviation from planarity which can be broadly rationalized in terms of different preferential bond lengths for Ca and O with substrate O and Ba (Sr), respectively. The marked preferences for particular bond lengths lead to the formation of loops or gaps in non-square islands, areas where islands interact and along the mid-edges of large islands. Exchange with the much larger cations in the substrate is surprisingly facile. Our results indicate the difficulties of preparing sharp, ordered thin oxide films even at low temperatures.

  19. Exploring the Dark Universe: Constraint on dynamical dark energy models from CMB, BAO and Growth Rate Measurements

    CERN Document Server

    Rivera, Alexander Bonilla

    2016-01-01

    In order to explain the current acceleration of the universe, the fine tuning problem of the value of $\\Lambda$ and the cosmic coincidence problem, different alternative models have been proposed. We use the most recent observational data from CMB (Plank 13 + WMAP 9) and LSS (SDSS, WiggleZ, BOSS, CMASS) to put cosmological constraints on different dynamical dark energy models. We employ the CMB Shift Parameter, which traditionally has been used to constrain the main cosmological parameters of the standard model $\\Lambda CDM$. BAO data are also used including the cross-correlation WiggleZ-BOSS data (BW), which have the potential to strongly constrain the history of the cosmological expansion and the main properties of dark energy. Additionally we use data from Redshift-Space Distortions through Growth Parameter $A(z)=f(z)\\sigma_{8}(z)$ to put constraints on the variance in mass fluctuations $\\sigma_{8}$. We study the expansion history trougth H(z), q(z) and j(z) parameters (Obtained from an expansion in Taylor...

  20. Measurement of odor causing compounds geosmin and 2-MIB in drinking water%饮用水中异嗅物质-土臭素及二甲基异冰片的测定方法

    Institute of Scientific and Technical Information of China (English)

    马晓雁; 高乃云; 李青松; 乐林生; 吴今明; 陈国光

    2006-01-01

    导致饮用水土霉味的常见物质为土臭素(GSM)和二甲基异冰片(2-MIB),此两种物质通常在原水及饮用水中痕量存在,浓度低于几百ng/L.总结了用感官分析法和仪器分析法测定痕量GSM和2-MIB.对液液萃取、固相萃取、固相微萃取及各种衍生水样预处理方法进行了对比,评价了各种方法的优劣,并对异嗅物质分析方法的发展提出了展望.

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  17. Running of αs and mb in the MSSM

    Science.gov (United States)

    Harlander, R. V.; Mihaila, L.; Steinhauser, M.

    2007-09-01

    A consistent evolution of the strong coupling constant αs from MZ to the grand unified theory (GUT) scale is presented, involving three-loop running and two-loop decoupling. The two-loop transition from the MS¯- to the DR¯-scheme is properly taken into account. In the second part of the paper, the bottom quark mass in the DR¯-scheme at the electroweak/supersymmetry (SUSY) scale is evaluated with four-loop accuracy. We find that the three-loop effects are comparable to the experimental uncertainty both for αs and mb.

  18. Arbutin in Serratula quinquefolia M.B. (Asteraceae

    Directory of Open Access Journals (Sweden)

    Gerard Nowak

    2011-04-01

    Full Text Available Genus Serratula is known for the presence of steroid compounds which belong to the group of ecdysones. Former phytochemical works from the late sixties and early seventies indicate, in some Serratula species, the occurrence of a phenolic glycoside-arbutin. This has been confirmed in the present work through finding an α,β-arbutin anomer in Serratula quinquefolia M.B. New botanical data concerning the classification of genus Serratula suggest that the species in question should be regarded as belonging to genus Klasea (currently a section of genus Serratula. There has been an attempt to find a key to the chemical division within the taxons.

  19. Chlorobium tepidum mutant lacking bacteriochlorophyll c made by inactivation of the bchK gene, encoding bacteriochlorophyll c synthase

    DEFF Research Database (Denmark)

    Frigaard, Niels-Ulrik; Voigt, Ginny D; Bryant, Donald A

    2002-01-01

    , a fraction containing vestigial chlorosomes, denoted "carotenosomes," was partly purified by density centrifugation; these structures contained carotenoids, isoprenoid quinones, and a 798-nm-absorbing BChl a species that is probably protein associated. Because of the absence of the strong BChl c absorption...... found in the wild type, the bchK mutant should prove valuable for future analyses of the photosynthetic reaction center and of the roles of BChl a in photosynthesis in green bacteria. An evolutionary implication of our findings is that the photosynthetic ancestor of green sulfur bacteria could have...

  20. The clinical utility of CK-MB measurement in patients suspected of acute coronary syndrome.

    Science.gov (United States)

    Kim, Jaehyup; Hashim, Ibrahim A

    2016-05-01

    This study aims to assess the clinical utility of CK-MB measurement in patients suspected of acute coronary syndrome (ACS). All CK-MB and troponin T measurements performed MB concentrations were increased whereas troponin T concentrations were negative at MB result. In this group, the discordant normal CK-MB lowered suspicion for ACS in only 22 cases (2.7%). Most common interpretations for isolated positive troponin were demand ischemia and impaired renal function. In most cases, discordant CK-MB results were not considered a significant finding. In the setting of suspected ACS, CK-MB has limited clinical impact when contemporary troponin assay results are available. Copyright © 2016 Elsevier B.V. All rights reserved.

  1. Measurements of radiation effects on a 4 Mb PSRAM memory

    Science.gov (United States)

    Gonçalez, Odair Lelis; Pereira Junior, Evaldo Carlos Fonseca; Vaz, Rafael Galhardo; Pereira, Marlon Antonio; Wirth, Gilson Inácio; Both, Thiago Hanna

    2014-11-01

    The results of a static test of total ionizing dose (TID) effects on an ISSI 4Mb PSRAM memory are reported in this work. The irradiation was performed at the IEAv's Laboratory of Ionizing Radiation with 1.17 and 1.32 MeV gamma-rays from a 60Co source at a dose rate of 2.5 krad/h up to an accumulated dose of 215.7 krad. The TID threshold for bit flip found in this experiment was 52.5 krad. From a sampling of 4096 memory addresses it was estimated a bit flip rate of approximately 50% at an accumulated dose of 215.7 krad.

  2. Direct Sensitivity Test of the MB/BacT System

    Directory of Open Access Journals (Sweden)

    Barreto Angela Maria Werneck

    2002-01-01

    Full Text Available In order to evaluate the direct-method test of sensitivity to drugs used in the principal tuberculosis treatment regimes, in the Organon Teknika MB/BacT system, we tested 50 sputum samples positive to microscopy taken from patients with pulmonary tuberculosis and with clinical indications for an antibiogram, admitted sequentially for examination during the routine of the reference laboratory. The material was treated v/v with 23% trisodium phosphate solution, incubated for 24 h at 35°C, and neutralized v/v with 20% monosodium phosphate solution. The material was then centrifuged and the sediment inoculated into flasks containing Rifampin - 2 µg/ml, Isoniazid - 0.2 µg/ml, Pyrazinamide - 100 µg/ml, Ethambutol - 2.5 µg/ml, Ethionamide - 1.25 µg/ml, and Streptomycin - 2 µg/ml. The tests were evaluated using the indirect method in the BACTEC 460 TB (Becton Dickinson system as the gold standard. The results showed that the Rifampin test performed best, i.e., 100% sensitivity at 95% Confidence Interval (82.2-100 and 100% specificity at 95% Confidence Interval (84.5-100, followed by Isoniazid and Pyrazinamide. In this experiment, 92% of the materials showed a final reading in 30 days; this period represents the time for primary isolation as well as the results of the sensitivity profile, and is within Centers for Disease Control and Prevention recommendations regarding time for performance of the antibiogram. The inoculated flasks showed no contamination during the experiment. The MB/BacT is shown to be a reliable, rapid, fully automated nonradiometric system for the tuberculosis antibiogram.

  3. Challenge study of the pathogen reduction capacity of the THERAFLEX MB-Plasma technology.

    Science.gov (United States)

    Reichenberg, S; Gravemann, U; Sumian, C; Seltsam, A

    2015-08-01

    Although most pathogen reduction systems for plasma primarily target viruses, bacterial contamination may also occur. This study aimed to investigate the bacterial reduction capacity of a methylene blue (MB) treatment process and its virus inactivation capacity in lipaemic plasma. Bacterial concentrations in plasma units spiked with different bacterial strains were measured before and after the following steps of the THERAFLEX MB-Plasma procedure: leucocyte filtration, MB/light treatment and MB filtration. Virus inactivation was investigated for three virus types in non-lipaemic, borderline lipaemic and highly lipaemic plasma. Leucocyte filtration alone efficiently eliminated most of the tested bacteria by more than 4 logs (Staphylococcus epidermidis and Staphylococcus aureus) or to the limit of detection (LOD) (≥ 4.8 logs; Escherichia coli, Bacillus cereus and Klebsiella pneumoniae). MB/light and MB filtration further reduced Staphylococcus epidermidis and Staphylococcus aureus to below the LOD. The small bacterium Brevundimonas diminuta was reduced by 1.7 logs by leucocyte filtration alone, and to below the LOD by additional MB/light treatment and MB filtration (≥ 3.7 logs). Suid herpesvirus 1, bovine viral diarrhoea virus and human immunodeficiency virus 1 were efficiently inactivated by THERAFLEX MB-Plasma, independent of the degree of lipaemia. THERAFLEX MB-Plasma efficiently reduces bacteria, mainly via the integrated filtration system. Its virus inactivation capacity is sufficient to compensate for reduced light transparency due to lipaemia. © 2015 International Society of Blood Transfusion.

  4. Loquat (Eriobotrya japonica) leaf extract inhibits the growth of MDA-MB-231 tumors in nude mouse xenografts and invasion of MDA-MB-231 cells.

    Science.gov (United States)

    You, Mi-Kyoung; Kim, Min-Sook; Jeong, Kyu-Shik; Kim, Eun; Kim, Yong-Jae; Kim, Hyeon-A

    2016-04-01

    The present study was conducted to examine the inhibitory effect of loquat leaves on MDA-MB-231 cell proliferation and invasion. Female athymic nude mice were given a subcutaneous (s.c.) inoculation of MDA-MB-231 cells and randomly grouped to receive a s.c. injection of either 500 mg/kg ethanol, water extract or vehicle five times a week. Tumor growth, mitotic rate and necrosis were examined. MDA-MB-231 cells were cultured with DMSO or with various concentrations of loquat water or ethanol extract. Proliferation, adhesion, migration, invasion and matrix metalloproteinase (MMP) activity were examined. Tumor growth of xenograft nude mouse was significantly reduced by loquat extracts. The results of mitotic examination revealed that loquat extracts reduced tumor cell division. Both ethanol and water extracts significantly inhibited MDA-MB-231 cell proliferation. The protein expression of ErbB3 was significantly down-regulated by loquat leaf extracts. Loquat leaf extracts increased apoptosis of MDA-MB-231 cells following 24 hour incubation and the ethanol extract was more potent in inducing apoptosis than the water extract. Furthermore, loquat extracts inhibited adhesion, migration and invasion of MDA-MB-231 cells. MMP activity was significantly inhibited by loquat extracts. Our results show that extracts of loquat inhibit the growth of tumor in MDA-MB-231 xenograft nude mice and the invasion of human breast cancer cells, indicating the inhibition of tumor cell proliferation and invasion.

  5. Angiographic adverse events during percutaneous coronary intervention fail to predict creatine kinase-MB elevation.

    Science.gov (United States)

    Blankenship, James C; Islam, M Ashequl; Wood, G Craig; Iliadis, Elias A

    2004-09-01

    We attempted to determine if aggressive detection of angiographic adverse events during coronary intervention could predict subsequent creatine kinase (CK)-MB elevations. During coronary intervention, both fluoroscopy and cine angiography were used to detect angiographic adverse events. At least one angiographic adverse event occurred in 133/251 (53%) of procedures. CK-MB elevation occurred in 24% of procedures. Slow flow during the procedure (P=0.002) and chest discomfort at the end of the procedure (P=0.007) were the strongest predictors of CK-MB elevation. Among procedures with no angiographic adverse events, CK-MB elevation occurred in 15/121 (12%), accounting for 25% of CK-MB elevations. We conclude that CK-MB elevation occurs after angiographically uncomplicated coronary interventions even when angiographic adverse events are aggressively detected. Routine monitoring of cardiac enzymes is necessary to detect all patients who will experience myocardial injury after coronary intervention.

  6. [Immunological safety and sensitizing effect of an MB-7-based vaccine against hepatitis A].

    Science.gov (United States)

    Smerdova, M A; Usova, S V; Smolina, M P; Netesov, S V; Maĭdaniuk, A G; Muntianova, M A; Nemtsov, Iu V; Kriuk, N I; Iashin, V V; Karpovich, L G; Kalashnikova, T V

    2004-01-01

    The influence of a vaccine based on the MB-7 strain of hepatitis A virus (VP-MB-7) designed at the "Vector" Center of Virology and Biotechnology was studied. VP-MB-7 was found to provoke no allergic response and to have an activating effect on the specific and non-specific responses of cell and humoral immunity similar to those evoked by hepatitis A vaccine "Hep-A-in Vac".

  7. The clustering of galaxies in the SDSS-III Baryon Oscillation Spectroscopic Survey: BAO measurement from the LOS-dependent power spectrum of DR12 BOSS galaxies

    CERN Document Server

    Gil-Marín, Héctor; Cuesta, Antonio J; Brownstein, Joel R; Chuang, Chia-Hsun; Ho, Shirley; Kitaura, Francisco-Shu; Maraston, Claudia; Prada, Francisco; Rodríguez-Torres, Sergio; Ross, Ashely J; Schlegel, David J; Schneider, Donald P; Thomas, Daniel; Tinker, Jeremy L; Tojeiro, Rita; Magaña, Mariana Vargas; Zhao, Gong-Bo

    2015-01-01

    [abridged] We present an anisotropic analysis of the baryonic acoustic oscillation (BAO) scale in the twelfth and final data release of the Baryonic Oscillation Spectroscopic Survey (BOSS). We independently analyse the LOWZ and CMASS galaxy samples: the LOWZ sample contains contains 361\\,762 galaxies with an effective redshift of $z_{\\rm LOWZ}=0.32$, and the CMASS sample consists of 777\\,202 galaxies with an effective redshift of $z_{\\rm CMASS}=0.57$. We extract the BAO peak position from the monopole power spectrum moment, $\\alpha_0$, and from the $\\mu^2$ moment, $\\alpha_2$. We report $H(z_{\\rm LOWZ})r_s(z_d)=(11.64\\pm0.62)\\cdot10^3\\,{\\rm km}s^{-1}$ and $D_A(z_{\\rm LOWZ})/r_s(z_d)=6.85\\pm0.17$ with a cross-correlation coefficient of $r_{HD_A}=0.42$, for the LOWZ sample; and $H(z_{\\rm CMASS})r_s(z_d)=(14.56\\pm0.38)\\cdot10^3\\,{\\rm km}s^{-1}$ and $D_A(z_{\\rm CMASS})/r_s(z_d)=9.42\\pm0.13$ with a cross-correlation coefficient of $r_{HD_A}=0.51$, for the CMASS sample. We combine these results with the measurements...

  8. Countermeasures Bao'an District skilled personnel development%宝安区技能人才开发对策研究

    Institute of Scientific and Technical Information of China (English)

    梁敏华

    2015-01-01

    The highly skilled personnel of introduction and development of enterprises to achieve the transformation and upgrading of an important guarantee to promote the development of highly skilled personnel groups,is an important task for human resources development strategy.To strengthen the rapid economic development leap in Bao'an District,we must first strengthen the skills of personnel development and training, the author of this article will Bao'an District of skilled personnel for development do some analysis and research.%高技能人才的引进和开发是企业实现转型升级的重要保障,推动高技能人才群体发展,是当前人才资源开发战略的重要任务.加强宝安区的经济发展的快速飞跃,首先要加强技能人才的开发和培训,本文笔者将对于宝安区技能人才开发做一定的分析和研究.

  9. Consolidated Development Objectives Document (CDOD) For MB-60

    Science.gov (United States)

    Greene, William D.

    2013-01-01

    This document defines the objectives related to liquid rocket engine system development to be undertaken by JAXA in support of the Space Launch System (SLS) Program managed out of the NASA Marshall Space Flight Center (MSFC). These objectives include furnishing the necessary management, labor, facilities, tools, equipment, and materials required to execute the specified activities. 1.1 Project Scope: The scope of this effort is to develop a rocket engine and associated products per the objectives and technical requirements established in this document. This engine, minus the engine controller, designated here as MB ]60, is to be developed through to a prequalification point of maturity. It is assumed that should JCNE ]1 development proceed beyond this maturity point towards actual flight qualification, the engine controller will be supplied and integrated by NASA. 1.2 Document Structure: The structure of this Consolidated Development Objectives Document (CDOD) includes a traditional description of objectives in a SOO, plus the associated Data Products Document (DPD) in an attached appendix, and then Engine Requirements Document (ERD) as another attached appendix. It is the intent that this document, in conjunction with the cited applicable documents, should constitute a complete programmatic and technical description of the development effort to be pursued.

  10. XAV939-mediated ARTD activity inhibition in human MB cell lines.

    Directory of Open Access Journals (Sweden)

    Cristiano Renna

    Full Text Available Diphtheria toxin-like ADP-ribosyltransferases 1 and 5 (ARTD-1, ARTD-5 are poly ADP-ribose enzymes (PARP involved in non-homologous end-joining (NHEJ, which is the major pathway of double-strand break (DSB repair. In addition, ARTD-5, or Tankyrase (TNKS, is a positive regulator of the WNT signaling implicated in the development and biological behavior of many neoplasms, such as Medulloblastoma (MB, in which radiotherapy is an essential part of the treatment. The use of radiosensitizing agents may improve the therapeutic index in MB patients by increasing the efficacy of radiotherapy, while reducing toxicity to the neuroaxis. ARTD-5 seems to be a good molecular target for improving the current treatment of MB. In this study, we used the small molecule XAV939, a potent ARTD-5 inhibitor with a slight affinity for ARTD-1, in different human MB cell lines. XAV939 inhibited the WNT pathway and DNA-PKcs in our MB cells, with many biological consequences. The co-administration of XAV939 and ionizing radiations (IR inhibited MB cells proliferation and clonogenic capacity, decreased their efficacy in repairing DNA damage, and increased IR-induced cell mortality. In conclusion, our in vitro data show that XAV939 could be a very promising small molecule in MB treatment, and these results lay the basis for further in vivo studies with the aim of improving the current therapy available for MB patients.

  11. XAV939-mediated ARTD activity inhibition in human MB cell lines.

    Science.gov (United States)

    Renna, Cristiano; Salaroli, Roberta; Cocchi, Claudia; Cenacchi, Giovanna

    2015-01-01

    Diphtheria toxin-like ADP-ribosyltransferases 1 and 5 (ARTD-1, ARTD-5) are poly ADP-ribose enzymes (PARP) involved in non-homologous end-joining (NHEJ), which is the major pathway of double-strand break (DSB) repair. In addition, ARTD-5, or Tankyrase (TNKS), is a positive regulator of the WNT signaling implicated in the development and biological behavior of many neoplasms, such as Medulloblastoma (MB), in which radiotherapy is an essential part of the treatment. The use of radiosensitizing agents may improve the therapeutic index in MB patients by increasing the efficacy of radiotherapy, while reducing toxicity to the neuroaxis. ARTD-5 seems to be a good molecular target for improving the current treatment of MB. In this study, we used the small molecule XAV939, a potent ARTD-5 inhibitor with a slight affinity for ARTD-1, in different human MB cell lines. XAV939 inhibited the WNT pathway and DNA-PKcs in our MB cells, with many biological consequences. The co-administration of XAV939 and ionizing radiations (IR) inhibited MB cells proliferation and clonogenic capacity, decreased their efficacy in repairing DNA damage, and increased IR-induced cell mortality. In conclusion, our in vitro data show that XAV939 could be a very promising small molecule in MB treatment, and these results lay the basis for further in vivo studies with the aim of improving the current therapy available for MB patients.

  12. Non-Gaussian diffusion MR imaging of glioma: comparisons of multiple diffusion parameters and correlation with histologic grade and MIB-1 (Ki-67 labeling) index

    Energy Technology Data Exchange (ETDEWEB)

    Yan, Ren; Haopeng, Pang; Xiaoyuan, Feng; Jiawen, Zhang; Zhenwei, Yao [Fudan University, Department of Radiology, Huashan Hospital, Shanghai (China); Jinsong, Wu; Chengjun, Yao; Tianming, Qiu [Fudan University, Department of Neurosurgery, Huashan Hospital, Shanghai (China); Ji, Xiong [Fudan University, Department of Neuropathology, Huashan Hospital, Shanghai (China); Mao, Sheng; Yueyue, Ding [Department of Imaging, Suzhou Children' s Hospital, Suzhou, Jiangsu (China); Yong, Zhang [MR Research, GE Healthcare, Shanghai (China); Jianfeng, Luo [Fudan University, Department of Biostatistics, Public Health School, Shanghai (China)

    2016-02-15

    This study was conducted to compare the association of Gaussian and non-Gaussian magnetic resonance imaging (MRI)-derived parameters with histologic grade and MIB-1 (Ki-67 labeling) index (MI) in brain glioma. Sixty-five patients with pathologically confirmed glioma, who underwent diffusion-weighted MRI with 2 b values (0, 1000 s/mm{sup 2}) and 22 b values (≤5000 s/mm{sup 2}), respectively, were divided into three groups of grade II (n = 35), grade III (n = 8), and grade IV (n = 22). Comparisons by two groups were made for apparent diffusion coefficient (ADC), slow diffusion coefficient (Dslow), distributed diffusion coefficient (DDC), and heterogeneity index α. Analyses of receiver operating characteristic (ROC) curve were performed to maximize the area under the curve (AUC) for differentiating grade III + IV (high-grade glioma, HGG) from grade II (low-grade glioma, LGG) and grade IV (glioblastoma multiforme, GBM) from grade II + III (other grade glioma, OGG). Correlations with MI were analyzed for the MRI parameters. On tumor regions, the values of ADC, Dslow, DDC, and α were significantly higher in grade II [(1.37 ± 0.29, 0.70 ± 0.11, 1.39 ± 0.34) (x 10{sup -3} mm{sup 2}/s) and 0.88 ± 0.05, respectively] than in grade III [(0.99 ± 0.13, 0.55 ± 0.07, 1.04 ± 0.20) (x 10{sup -3} mm{sup 2}/s) and 0.80 ± 0.03, respectively] and grade IV [(1.03 ± 0.14, 0.50 ± 0.05, 1.02 ± 0.16) (x 10{sup -3} mm{sup 2}/s) and 0.76 ± 0.04, respectively] (all P < 0.001). The parameter α showed the highest AUCs of 0.950 and 0.922 in discriminating HGG from LGG and GBM from OGG, respectively. Significant correlations with histologic grade and MI were observed for the MRI parameters. The non-Gaussian MRI-derived parameters α and Dslow are superior to ADC in glioma grading, which are comparable with ADC as reliable biomarkers in noninvasively predicting the proliferation level of glioma malignancy. (orig.)

  13. Gas Phase Structure of Amino Acids: La-Mb Studies

    Science.gov (United States)

    Mata, I. Pena S.; Sanz, M. E.; Vaquero, V.; Cabezas, C.; Perez, C.; Blanco, S.; López, J. C.; Alonso, J. L.

    2009-06-01

    Recent improvements in our laser ablation molecular beam Fourier transform microwave (LA-MB-FTMW) spectrometer such as using Laval-type nozzles and picoseconds Nd:YAG lasers (30 to 150 ps) have allowed a major step forward in the capabilities of this experimental technique as demonstrated by the last results in serine cysteine and threonine^a for which seven, six and seven conformers have been respectively identified. Taking advantage of these improvements we have investigated the natural amino acids metionine, aspartic and glutamic acids and the γ-aminobutyric acid (GABA) with the aim of identify and characterize their lower energy conformers. Searches in the rotational spectra have lead to the identification of seven conformers of metionine, six and five of aspartic and glutamic acids, respectively, and seven for the γ-aminobutyric. These conformers have been unambiguously identified by their spectroscopic constants. In particular the ^{14}N nuclear quadrupole coupling constants, that depend heavily on the orientation of the amino group with respect to the principal inertial axes of the molecule, prove to be a unique tool to distinguish unambigously between conformations with similar rotational constants. For the γ-aminobutyric acid two of the seven observed structures are stablized by an intramolecular interaction n-π*. Two new conformers of proline have been identified together with the two previously observed. J. L. Alonso, C. Pérez, M. E. Sanz, J. C. López, S. Blanco, Phys.Chem.Chem.Phys., 2009, 11, 617. D. B. Atkinson, M. A. Smith, Rev. Sci. Instrum. 1995, 66, 4434 S. Blanco, M. E. Sanz, J. C. López, J. L. Alonso, Proc. Natl. Acad. Sci. USA2007, 104, 20183. M. E. Sanz, S. Blanco, J. C. López, J. L. Alonso, Angew. Chem. Int. Ed.,2008, 120, 6312. A. Lesarri, S. Mata, E. J. Cocinero, S. Blanco, J.C. López, J. L. Alonso, Angew. Chem. Int. Ed. , 2002, 41, 4673

  14. 基于模糊层次分析法的余额宝风险评价%Risk Assessment of Yu’eBao Based on Fuzzy Analytic Hierarchy Process

    Institute of Scientific and Technical Information of China (English)

    殷敏

    2015-01-01

    In this paper we systematically analyzed the risks of Yu’eBao,constructed the risk assessment model based on AHP and Fuzzy Comprehensive Evaluation,and assessed the effect of each index on the risks of Yu’eBao. The resutts showed that the risk of Yu’eBao is high,and the revenue risk,redeeming risk,regulatory risk,interest-rate risk,bank run risk are the most important indicators and the main risks of Yu’eBao management and precaution.%对余额宝存在的风险进行了系统分析,并利用层次分析法和模糊综合评价法,构建了余额宝风险评价模型,分析评价各个指标对余额宝整体风险的影响,得出余额宝不仅具有较高的风险,而且收益风险、赎回风险、监管风险、利率风险、银行挤兑风险等是余额宝风险评价最重要的指标,也是余额宝应重点管理和防范的风险。

  15. The smallsat TIR spectrometer MIBS

    NARCIS (Netherlands)

    Leijtens, J.A.P.; Court, A.J.; Lucas, J.W.

    2005-01-01

    In frame of the ESA Earthcare MSI study, TNO Science and Industry has developed a compact spectrometer which is optimized for operation in the 7 to 14 μm wavelength region. By optimizing the throughput of the system, and using the advantages of modern manufacturing technologies to the largest extend

  16. Retraction: "Over-expression of FoxM1 leads to epithelial-mesenchymal transition and cancer stem cell phenotype in pancreatic cancer cells" by Bao et al.

    Science.gov (United States)

    2016-08-01

    The above article, published online on April 18, 2011 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the journal Editor in Chief, Gary S. Stein, and Wiley Periodicals, Inc. The retraction has been agreed following an investigation from Wayne State University involving the second author that found Figures 1C and 4C to be inappropriately re-used and re-labeled. REFERENCE Bao B, Wang Z, Ali S, Kong D, Banerjee S, Ahmad A, Li Y, Azmi AS, Miele L, Sarkar FH. 2011. Over-expression of FoxM1 leads to epithelial-mesenchymal transition and cancer stem cell phenotype in pancreatic cancer cells. J Cell Biochem 112:2296-2306; doi: 10.1002/jcb.23150.

  17. Protection from procedural myocardial injury by omega-3 polyunsaturated fatty acids (PUFAs): is related with lower levels of creatine kinase-MB (CK-MB) and troponin I?

    Science.gov (United States)

    Foroughinia, Farzaneh; Salamzadeh, Jamshid; Namazi, Mohammad H

    2013-10-01

    This study sought to investigate the effect of omega-3 polyunsaturated fatty acids (PUFAs) on cardiac biomarkers, CK-MB, and troponin I in patients undergoing PCI. Restenosis remains as a major long-term complication following percutaneous coronary intervention (PCI). It appears that there is strong relationship between post-PCI creatine kinase-MB (CK-MB) and troponin I elevation and cardiovascular events after PCI. In this randomized clinical trial, a total of 90 patients planned to undergo PCI were randomly assigned into two groups: Group A-receiving omega-3 PUFAs (3 g, 12 h before PCI) plus standard treatment (n = 43) and Group B-control group, receiving only standard therapy (n = 47). Standard treatment included aspirin 325 mg and clopidogrel 600 mg loading dose. The plasma CK-MB level was measured before the procedure (baseline), at 8 and 24 h after PCI. The plasma troponin I was measured at baseline and 24 h after PCI. In comparison with control, omega-3 PUFAs could significantly reduce the level of CK-MB in 8 (P = 0.001) and 24 h (P = 0.012) after its prescription in the omega-3 PUFAs group. Omega-3 PUFAs could not significantly decrease troponin I. Our results revealed that omega-3 PUFAs can be considered as a safe adjunctive medication to the standard regimen before PCI for the aim of decreasing cardiovascular event after PCI. © 2012 John Wiley & Sons Ltd.

  18. Creatine Kinase (CK)-MB-to-Total-CK Ratio: a Laboratory Indicator for Primary Cancer Screening.

    Science.gov (United States)

    Chang, Chih-Chun; Liou, Ching-Biau; Su, Ming-Jang; Lee, Yi-Chen; Liang, Chai-Ting; Ho, Jung-Li; Tsai, Huang-Wen; Yen, Tzung-Hai; Chu, Fang-Yeh

    2015-01-01

    For the determination of creatine kinase (CK)-MB, the immunoinhibition method is utilized most commonly. However, the estimated CK-MB activity may be influenced by the presence of CK isoenzymes in some conditions like cancer. Thus, a CK-MB-to-total-CK ratio more than 1.0 could be found in such a situation. The study aimed to explore the relationship of cancer to high CK-MB-to-total-CK ratio. From January 2011 to December 2014, laboratory data on all CK-MB and total CK test requests were extracted at Far Eastern Memorial Hospital (88,415 requests). Patients with a CK-MB-to-total-CK ratio more than 1.0 were registered in this study. Clinical data including tumor location, tumor TNM stage and metastatic status were also collected. A total of 846 patients were identified with a CK-MB-to-total-CK ratio more than 1.0. Of these, 339 (40.1%) were diagnosed with malignancies. The mean CK-MB-to-total-CK ratio was significantly higher in malignancy than in non-malignancy (1.35±0.28 vs 1.25±0.23, pMB-to-total-CK ratio more than 1.0 was colorectal cancer (1.42±0.28, 16.5%, n=56), followed by lung cancer (1.38±0.24, 15.9%, n=54) and hepatocellular carcinoma (14.5%, n=49). Higher CK-MB-to-total-CK ratios in hematological malignancies (1.44±0.41)were also noted. Additionally, the CK-MB-to-total-CK ratio was markedly higher in advanced stage malignancy than in early stage (1.37±0.26 vs. 1.29±0.31, p=0.014) and significantly higher in liver metastasis than in non-liver metastasis (1.48±0.30 vs. 1.30±0.21, pMB-to-total-CK ratio is an easily available indicator and could be clinically utilized as a primary screening tool for cancer. Higher ratio of CK-MB-to-total-CK was specifically associated with certain malignancies, like colorectal cancer, lung cancer and hepatocellular carcinoma, as well as some cancer-associated status factors such as advanced stage and liver metastasis.

  19. On the Huizhou Clans and the Pursuing of the Bao-Jia System in the Ming and Qing Dynasties%明清时期徽州的宗族与保甲推行

    Institute of Scientific and Technical Information of China (English)

    陈瑞

    2012-01-01

    明清时期徽州境内的保甲推行得到了当地宗族组织的支持与配合。徽州宗族推行保甲的举措主要包括:(一)在族内实行门房支派、家户、家丁等不同层级的轮流承管制度,以分担保甲之役;(二)通过摊派或鼓励捐助等方式在族内设立保甲银等固定基金,对承充保甲之役人员实行津贴制,以确保保甲之役的顺利完成;(三)针对一些族人经商外地,难以及时承充保甲之役,在族内实行雇役制,商人则需承担相应的费用;(四)异姓宗族在保甲之役承充方面进行着有效合作,主要根据各自的人口、钱粮等实力因素,实行朋充或轮充制,以分担保甲之役。徽州宗族在保甲之役承充方面的合作.有较为细致明确的分工.灵活而富有弹性。由于保甲制的推行和保甲组织的运作具有浓厚的官方色彩和官方背景,因而,明清时期徽州宗族与保甲之间的良性互动,实质上是徽州族权与封建政权之间良性互动关系的一种反映。%The in the Ming and Qing pursuing of the Bao-Jia system obtained the support Dynasties. The measures of Huizhou clans pursuing and coordination from Huizhou clans the Bao-Jia system contained: First- ly, in order to share the Bao-Jia corv 6 e, the lunchong (on duty by turns)system had been carried out by each branch, household or population within the Huizhou clans. Scondly, in order to finish the burdensome tasks from feudal official, the fixed funds (e.g. Bao-Jia silver) which served as extra allowance for the Bao-Jia corv 6 e, had been set up by apportion or donation within the Huizhou clans. Thirdly, in order to finish the Bao-Jia corv 6 e taken by out-of-town merchants, all kinds of flexible wage labourer systems had been carried out, and merchants must accept relative expense within the Huizhou clans. Fourthly, in order to share the Bao-Jia corv 6 e, cooperations based on the fiscal

  20. CREATINE PHOSPHOKINASE ISOENZYME-MB MASS (CK-MB MASS AND TROPONIN I (cTnI IN DOGS (Canis familiaris

    Directory of Open Access Journals (Sweden)

    Millena Vidal Freitas

    2015-07-01

    Full Text Available Cardiac markers, especially CK-MB mass and cTnI, have an essential role in both human and veterinary clinical and surgical cardiology, allowing a more precise and accurate diagnosis in myocardial lesions. The goal of this work was to measure these cardiac markers in veterinary medicine, improve their use and provide information about these laboratory methods that allow non-invasive health monitoring of the myocardial cell. Parameters quantification was obtained from sera of healthy dogs examined during routine procedures at the Small Animal Veterinary Hospital of Universidade Estadual do Norte Fluminense Darcy Ribeiro. The human chemiluminescence essay turbo kit (IMMULITE Turbo, Siemens ® test proved to be effective in canine species for both CK-MB mass and cTnI. In addition, the values found for CK-MB will significantly contribute to clinical routine or experimental work with dogs.

  1. [Evaluation of the latex turbidimetric immunoassay for CK-MB protein].

    Science.gov (United States)

    Okubo, Manabu; Kimura, Shigeki; Matsui, Masahiko; Suehisa, Etsuji; Hidaka, Yoh

    2013-11-01

    CK-MB protein is an important marker for the diagnosis of acute coronary syndromes. In the present study, we evaluated the basal performance of recently developed CK-MB mass kit "L-type wako CK-MB mass". Within-run and between-day precision were obtained with 1.4-4.7% and 2.7-5.2%, respectively. Diluted linearity and lower limit of detection were obtained with 180.0 ng/mL and 2.1 ng/mL, respectively. Zone phenomenon was able to detect until 25,600.0 ng/mL. Analysis of interferent showed that only CK-BB positively influenced the assay results. CK-MB protein levels decreased to 82% at 72 hours in the room temperature, but it was stable at 4 degrees C and -20 degrees C. The correlation coefficient(r) between this assay and conventional assay was 0.999. However, discrepancy of the two cases was observed in the comparison between the two methods. In the case 1, CK isoenzyme analysis using electrophoresis indicated that CK-MB was not present and absorption test showed a 68% absorption effect of CK-MB protein values not by anti human IgG, anti human IgA, and animal serums, but anti human IgM. In the case 2, CK isoenzyme analysis indicated that there is not only CK-MB but CK-BB. CK-MB protein values between the two methods were fitted after decreasing CK-BB. Thus, Value discrepancy for CK-MB protein was resulting from IgM and CK-BB. We have to pay attention to such phenomenon when detecting an unlikely higher levels that could not be explained by clinical information.

  2. Can CK-MB be used as a marker in benign paroxysmal positional vertigo attack?

    Science.gov (United States)

    Torun, Mümtaz Taner; Yalçın, Yusuf; Özkan, Özalkan

    2017-04-19

    Benign paroxysmal positional vertigo (BPPV) is one of the main causes of vertigo. More than 90% of cases of positional vertigo and nystagmus can be associated with BPPV. There is no known specific laboratory test for BPPV. Because of the possible muscle activity in BPPV (especially result of nause, vomiting and increasing heart rate) we thought the CK-MB levels may change in this situation. The purpose of this study was to investigate the relation between serum CK-MB levels and BPPV and attacks. The medical records of 35 patients with BPPV presenting to our clinic between November 2014 and November 2015 were investigated retrospectively. Thirty-two control patients with no vertigo, muscular or cardiac disease and with measured CK-MB levels were randomized into a control group. Both groups' existing CK-MB levels were obtained from the patient records. CK-MB values measured before BPPV attack from 11 patients in the patient group were also recorded. The patient and control groups were similar in terms of demographic parameters. CK-MB values at time of attack in the patient group ranged between 5.4 and 38.9 (mean: 19.67 ± 6.81), while control group values ranged between 12 and 27.9 (mean: 20.31 ± 4.54). CK-MB values in 11 patients in the patient group measured before attack ranged between 8.9 and 24 (mean: 17.02 ± 4.97), and CK-MB values during attack ranged between 9 and 34 (mean: 19.74 ± 7.64). Although CK-MB elevation at time of attack was determined in patients with BPPV, this was not statistically significant.

  3. [Effect of autophagy inhibitor combined with EGFR inhibitor on triple-negative breast cancer MDA-MB-468 and MDA-MB-231 cells].

    Science.gov (United States)

    Liu, Z Y; He, K W; Song, X G; Wang, X Z; Zhuo, P Y; Wang, X W; Ma, Q H; Huo, Z J; Yu, Z Y

    2016-06-23

    To investigate the effect of combined administration of autophagy inhibitor 3-methyladenine/bafilomycin A1 and EGFR inhibitor gefitinib on triple-negative breast cancer MDA-MB-468, MDA-MB-231 cells and estrogen receptor-positive MCF-7 cells. All the cells were treated with 3-methyladenine/bafilomycin A1 and/or gefitinib. The effect of autophagy inhibitor and gefitinib on the cell growth was evaluated by MTT assay. Cell apoptosis was detected by flow cytometry. Western blot analysis was used to determine the alteration of autophagy-related protein (such as LC3) and apoptosis-related proteins (such as caspase-3 and caspase-9). MTT assay showed that the IC50 in the GE+ 3-MA and GE+ BAF groups were (4.1±0.2) μmol/L and (3.8±0.3) μmol/L, significantly lower than that of the gefitinib alone group [(7.0±0.2) μmol/L] in MDA-MB-468 cells (PMB231 cells (PMB-468 cells in GE, GE+ 3-MA and GE+ BAF groups were (12.43±3.18)%, (23.37±2.71)% and (18.71±2.81)%, respectively. The apoptosis rates of MDA-MB-231 cells of the GE, GE+ 3-MA and GE+ BAF groups were (12.15±1.82)%, (16.94±2.19)% and (33.83±5.92) %, significantly higher than that of the gefitinib alone group (All P0.05). Western blot data showed that the expression levels of LC3 and p-Akt were decreased in the combined groups than that of the gefitinib alone group, while the p-PTEN, caspase-3 and caspase-9 were increased. Autophagy inhibitor may enhance the sensitivity to gefitinib in MDA-MB-468 and MDA-MB-231 cells by activation of the PTEN/P13K/Akt pathway. Apoptosis in MDA-MB-468 and MDA-MB-231 cells might be enhanced by the combination treatment through caspase cascade.

  4. File list: InP.Brs.50.AllAg.MDA-MB-231 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.Brs.50.AllAg.MDA-MB-231 hg19 Input control Breast MDA-MB-231 SRX883581,SRX88358...46863,SRX1156474,SRX737089 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/InP.Brs.50.AllAg.MDA-MB-231.bed ...

  5. File list: NoD.Brs.10.AllAg.MDA-MB-231 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.Brs.10.AllAg.MDA-MB-231 hg19 No description Breast MDA-MB-231 ERX309839,ERX3098...45 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/NoD.Brs.10.AllAg.MDA-MB-231.bed ...

  6. File list: NoD.Brs.05.AllAg.MDA-MB-231 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.Brs.05.AllAg.MDA-MB-231 hg19 No description Breast MDA-MB-231 ERX309839,ERX3098...45 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/NoD.Brs.05.AllAg.MDA-MB-231.bed ...

  7. File list: InP.Brs.05.AllAg.MDA-MB-231 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.Brs.05.AllAg.MDA-MB-231 hg19 Input control Breast MDA-MB-231 SRX883581,SRX88358...56474,SRX332680,SRX1156475 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/InP.Brs.05.AllAg.MDA-MB-231.bed ...

  8. File list: NoD.Brs.50.AllAg.MDA-MB-231 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.Brs.50.AllAg.MDA-MB-231 hg19 No description Breast MDA-MB-231 ERX309839,ERX3098...45 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/NoD.Brs.50.AllAg.MDA-MB-231.bed ...

  9. File list: InP.Brs.10.AllAg.MDA-MB-231 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.Brs.10.AllAg.MDA-MB-231 hg19 Input control Breast MDA-MB-231 SRX883581,SRX88358...56474,SRX246863,SRX1156475 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/InP.Brs.10.AllAg.MDA-MB-231.bed ...

  10. File list: InP.Brs.20.AllAg.MDA-MB-231 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.Brs.20.AllAg.MDA-MB-231 hg19 Input control Breast MDA-MB-231 SRX883581,SRX88358...156475,SRX332680,SRX246863 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/InP.Brs.20.AllAg.MDA-MB-231.bed ...

  11. Adsorption Characteristics of 2-MIB Onto Powdered Activated Carbons in the Reservoir Type Source Water%PAC对水库型水源水中嗅味物质2-MIB的吸附特性

    Institute of Scientific and Technical Information of China (English)

    宋武昌; 李星; 潘章斌; 杨艳玲; 孙韶华; 贾瑞宝

    2015-01-01

    为解决水库型水源水中季节性藻致嗅味物质的应急处理问题,研究了不同水质条件下粉末活性炭( PAC)对水中嗅味物质二甲基异崁醇(2-MIB)的吸附动力学模型和吸附热力学模型,考察了2类水库型水源水中有机物对吸附作用的影响。结果表明:在试验浓度范围内,纯水、原水A和原水B中PAC对2-MIB的吸附过程符合准二级反应动力学模型,吸附等温线符合修正的Freundlich方程,且吸附速率和吸附容量依次降低;原水A、B中PAC对2-MIB的吸附能力显著降低,但原水B中2-MIB在活性炭表面的吸附势能比原水A中的强,这可能主要是由原水中分子质量<500 u的小分子有机物引起的微孔吸附竞争及其极性造成的。%In order to solve the problem of seasonal pollution of reservoir water by taste and odor caused by algae, the adsorption kinetics and isothermal adsorption modeling of 2-methylisoborneol (2-MIB) by powdered activated carbons ( PAC) in different water quality were studied, and the adsorption effects of organic matter in two kinds of reservoir water were investigated. Results showed that, in the range of test concentration, the adsorption processes followed pseudo-second-order kinetics equation and the modified Freundlich equation in pure water, raw water A and B, and the adsorption rate and adsorption capacity reduced in turn. The adsorption capacity decreased significantly in raw water A and B, but the adsorption potential energies of 2-MIB on activated carbon in raw water B was stronger than that of raw water A, due to the micropore competitive adsorption and polarity caused by the reservoir water of small organic molecules with relative molecular weight of <500 u.

  12. Action and Signaling of Lysophosphatidylethanolamine in MDA-MB-231 Breast Cancer Cells.

    Science.gov (United States)

    Park, Soo-Jin; Lee, Kyoung-Pil; Im, Dong-Soon

    2014-02-01

    Previously, we reported that lysophosphatidylethanolamine (LPE), a lyso-type metabolite of phosphatidylethanolamine, can increase intracellular Ca(2+) ([Ca(2+)]i) via type 1 lysophosphatidic acid (LPA) receptor (LPA1) and CD97, an adhesion G-protein-coupled receptor (GPCR), in MDA-MB-231 breast cancer cells. Furthermore, LPE signaling was suggested as like LPA1/CD97-Gi/o proteins-phospholipase C-IP3-Ca(2+) increase in these cells. In the present study, we further investigated actions of LPE not only in the [Ca(2+)]i increasing effect but also in cell proliferation and migration in MDA-MB-231 breast cancer cells. We utilized chemically different LPEs and a specific inhibitor of LPA1, AM-095 in comparison with responses in SK-OV3 ovarian cancer cells. It was found that LPE-induced Ca(2+) response in MDA-MB-231 cells was evoked in a different manner to that in SK-OV3 cells in terms of structural requirements. AM-095 inhibited LPE-induced Ca(2+) response and cell proliferation in MDA-MB-231 cells, but not in SK-OV3 cells, supporting LPA1 involvement only in MDA-MB-231 cells. LPA had significant effects on cell proliferation and migration in MDA-MB-231 cells, whereas LPE had less or no significant effect. However, LPE modulations of MAPKs (ERK1/2, JNK and p38 MAPK) was not different to those by LPA in the cells. These data support the involvement of LPA1 in LPE-induced Ca(2+) response and cell proliferation in breast MDA-MB-231 cells but unknown GPCRs (not LPA1) in LPE-induced responses in SK-OV3 cells. Furthermore, although LPE and LPA utilized LPA1, LPA utilized more signaling cascades than LPE, resulting in stronger responses by LPA in proliferation and migration than LPE in MDA-MB-231 cells.

  13. STS-48 MS Gemar uses laptop during training session in JSC's MB SMS

    Science.gov (United States)

    1991-01-01

    STS-48 Discovery, Orbiter Vehicle (OV) 103, Mission Specialist (MS) Charles D. Gemar, wearing lightweight headset, enters data into a portable laptop computer on the middeck of JSC's Motion Based (MB) Shuttle Mission Simulator (SMS). Gemar is participating in a preflight familiarization session in the MB-SMS located in the Mission Simulation and Training Facility Bldg 5. Visible to Gemar's right is a stowed extravehicular mobility unit (EMU) and on his left are forward locker mockups.

  14. Assessment of Contaminated Brine Fate and Transport in MB139 at WIPP

    Energy Technology Data Exchange (ETDEWEB)

    Kuhlman, Kristopher L. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). Applied Systems Analysis and Research Dept.; Malama, Bwalya [Sandia National Lab., Carlsbad, NM (United States). Performance Assessment Dept.

    2014-07-01

    Following the radionuclide release event of February 14, 2014 at the Waste Isolation Pilot Plant (WIPP), actinide contamination has been found on the walls and floor in Panel 7 as a result of a release in Room 7 of Panel 7. It has been proposed to decontaminate Panel 7 at the WIPP by washing contaminated surfaces in the underground with fresh water. A cost-effective cleanup of this contamination would allow for a timely return to waste disposal operations at WIPP. It is expected that the fresh water used to decontaminate Panel 7 will flow as contaminated brine down into the porosity of the materials under the floor – the run-of-mine (ROM) salt above Marker Bed 139 (MB139) and MB139 itself – where its fate will be controlled by the hydraulic and transport properties of MB139. Due to the structural dip of MB139, it is unlikely that this brine would migrate northward towards the Waste-Handling Shaft sump. A few strategically placed shallow small-diameter observation boreholes straddling MB139 would allow for monitoring the flow and fate of this brine after decontamination. Additionally, given that flow through the compacted ROM salt floor and in MB139 would occur under unsaturated (or two-phase) conditions, there is a need to measure the unsaturated flow properties of crushed WIPP salt and salt from the disturbed rock zone (DRZ).

  15. Study of CK-MB activity in patients with acute myocardial infarction after percutaneous coronary intervention.

    Science.gov (United States)

    Emukhvari, N M; Tsetsekhladze, E D; Khijakadze, Kh A; Mamatsashvili, I O; Napetvaridze, R G

    2015-02-01

    The research has been carried out in patients of TSMU Cardiovascular Department of A.Aladashvili University Clinic. 105 patients with acute myocardial infarction have been involved in the study, wich undergoing percutaneous coronary intervention (PCI). For several years coronary angioplasty has been proposed to be an effective method, but in spite of its well developed technique, probability of myocardial injury is still high which appears to have no clinical or electrocardiographic manifestations and is diagnosed only by elevation of cardiac marker level. According to our study data after successful PCI elevation of CK-MB mass was observed in 34.4% patients, majority of those patients had STEMI. In II group the age of patients was higher compared to I group. There were more patients with diabetes mellitus (38.8%), dyslipidemia (86.1%) and patients with low left ventricular ejection fraction (50%). Also there were more patients with previous MI and damage of 3 coronary arteries. Hence age, diabetes mellitus, dyslipidemia, left ventricular ejection fraction MB elevation after successful PCI. Solid elevation of CK-MB after procedure was also associated with increased hospital complications rate, 30-day and 6 months hospitalization rate and 6 months mortality rate. It should be noted that from 36 patients who developed solid (24 h) elevation of CK-MB after PCI CK-MB mass was increased in all cases, while the concentration was elevated only in 16 cases. It proves that CK-MB mass is more significant criteria of myocardial injury.

  16. Removal of Stinking Compounds Geosmin (GSM) and 2-Methylisoborneol (MIB) in Drinking Water%饮用水中嗅味物质-土臭素和二甲基异冰片去除技术

    Institute of Scientific and Technical Information of China (English)

    陈蓓蓓; 高乃云; 马晓雁; 徐斌

    2007-01-01

    本文介绍了引起饮用水臭味的痕量污染物土臭素(geosmin,GSM)和二甲基异冰片(2-methylisoborneol,MIB)的来源和危害,分析了吸附、臭氧氧化、光催化氧化、生物氧化等工艺对它们的去除效果.针对原水中不同种类和不同浓度的嗅味物质,应采取不同的处理工艺,达到良好的处理效果,满足居民对水质的要求.

  17. Determination of geosmin and 2-MIB by SPE-GC%固相萃取-气相色谱法测定土臭素和2-甲基异冰片

    Institute of Scientific and Technical Information of China (English)

    李福安; 张焕伟; 巢猛; 曾东宝; 严锦玲

    2011-01-01

    研究了固相萃取-气相色谱法(GC/FID)测定饮用水中微量的土臭素和2-甲基异冰片(2-MIB)的试验条件.结果表明,用SPE C18固相萃取小柱处理250 mL水样,用甲醇洗脱并定容至1 mL,取1 μL进样至气相色谱仪,土臭素最低检出质量浓度为0.05μg/L,2-甲基异冰片最低检出质量浓度为0.10μg/L.

  18. Metabolic changes associated with methionine stress sensitivity in MDA-MB-468 breast cancer cells.

    Science.gov (United States)

    Borrego, Stacey L; Fahrmann, Johannes; Datta, Rupsa; Stringari, Chiara; Grapov, Dmitry; Zeller, Michael; Chen, Yumay; Wang, Ping; Baldi, Pierre; Gratton, Enrico; Fiehn, Oliver; Kaiser, Peter

    2016-01-01

    The majority of cancer cells have a unique metabolic requirement for methionine that is not observed in normal, non-tumorigenic cells. This phenotype is described as "methionine dependence" or "methionine stress sensitivity" in which cancer cells are unable to proliferate when methionine has been replaced with its metabolic precursor, homocysteine, in cell culture growth media. We focus on the metabolic response to methionine stress in the triple negative breast cancer cell line MDA-MB-468 and its methionine insensitive derivative cell line MDA-MB-468res-R8. Using a variety of techniques including fluorescence lifetime imaging microscopy (FLIM) and extracellular flux assays, we identified a metabolic down-regulation of oxidative phosphorylation in both MDA-MB-468 and MDA-MB-468res-R8 cell types when cultured in homocysteine media. Untargeted metabolomics was performed by way of gas chromatography/time-of-flight mass spectrometry on both cell types cultured in homocysteine media over a period of 2 to 24 h. We determined unique metabolic responses between the two cell lines in specific pathways including methionine salvage, purine/pyrimidine synthesis, and the tricarboxylic acid cycle. Stable isotope tracer studies using deuterium-labeled homocysteine indicated a redirection of homocysteine metabolism toward the transsulfuration pathway and glutathione synthesis. This data corroborates with increased glutathione levels concomitant with increased levels of oxidized glutathione. Redirection of homocysteine flux resulted in reduced generation of methionine from homocysteine particularly in MDA-MB-468 cells. Consequently, synthesis of the important one-carbon donor S-adenosylmethionine (SAM) was decreased, perturbing the SAM to S-adenosylhomocysteine ratio in MDA-MB-468 cells, which is an indicator of the cellular methylation potential. This study indicates a differential metabolic response between the methionine sensitive MDA-MB-468 cells and the methionine insensitive

  19. BAO and Its Application on Analysis of Business Location%BAO及在商业选址分析中的应用

    Institute of Scientific and Technical Information of China (English)

    刘云; 吴健平

    2013-01-01

    商业选址与布局关系到企业未来的规划与发展,是企业经营战略的重中之重。由于传统分析研究方法的局限,促使现代商业网点的选址迫切地需要新技术的支持以适应现代商业经营方式的根本性转变。为了降低企业成本、获取更多的市场份额以及更好地服务客户,文中分析了影响商业网点选址的人口、交通、市场竞争三个关键要素,介绍了Esri公司推出的在线商业选址应用-Business Analyst Online ( BAO),探讨商业选址分析的主要流程。通过在案例中应用BAO的主要功能,得出最佳商业选址,为企业决策者制定经营目标和经营策略提供了重要依据。%Related to future planning and development of the enterprise,business location and layout is the most important part in the man-agement strategy. Due to the limitations of the traditional research methods,business sites are crying for the new technologies in order to adapt to the fundamental shift of modern business models. In order to reduce the cost of enterprises,obtain more market share and offer better service for the customers,analyze three key impacts of the commercial net location,that is to say,population,transportation and market competition. Business Analyst Online ( BAO) is introduced with its main process of business location and examples. The best bus-iness location is acquired in the conclusion,which provides a reference for decision makers to make business objectives and strategy.

  20. The Dilemma of Regulation against Yu-E-Bao and Its Solution%余额宝规制的困境与出路

    Institute of Scientific and Technical Information of China (English)

    阳建勋

    2015-01-01

    余额宝的混业经营型创新金融产品特征、金融法律相对于互联网金融市场创新的滞后性等是导致余额宝规制困境——过度规制与规制缺位并存的主要原因。突破困境,要基于增利宝业务的众筹本质填补规制缺位,借鉴美国JOBS法案,将众筹纳入我国证券法的调整范围,从发行总额限制、投资者总额限制、发行人的信息披露义务及众筹经纪人与集资门户的注册要求等方面,建立和完善我国的众筹发行豁免注册制度;还要纠正与防范对余额宝的过度规制,具体路径是对余额宝规制措施进行全面的成本收益分析,最终以规制措施的总体效果作为取舍的标准。%There is a dilemma of regulation for Yu-E-Bao that is the coexistence of excessive regulation with absent regulation,most of reasons for which are Yu-E-Bao’s feature as a kind of mixed management innovation financial products,hysteresis quality of financial law relative to internet financial innovation.How to break through the dilemma? The absence of regulation should be filled on the basis of Zeng-Li-Bao’s essence of crowdfunding.In other words,crowdfunding should be adjusted by securities law in our country referred by the American JOBS Act and crowdfunding issuing exemption from registration systems should be established and improved such as issuer’s total amount limit,investor’s total amount limit,issuer’s information disclosure obligation and registration requirements for raising agent and raise portal.Secondly,excessive regulation against Yu-E-Bao should be corrected and prevented,the concrete path of which is to conduct a comprehensive cost-benefit analysis of regulation measures.At last the standard of trade-offs is the overall effect of regulation measures.

  1. [Effect of Aloe emodin on invasion and metastasis of high metastatic breast cancer MDA-MB-231 cells].

    Science.gov (United States)

    He, Zhen-Hui; Huang, Yue-Qun; Weng, Shan-Fan; Tan, Yao-Rong; He, Tai-Ping; Qin, Yan-Mei; Liang, Nian-Ci

    2013-09-01

    To investigate the effect of Aloe emodin (AE) on the invasive and metastatic abilities of human high metastatic breast cancer MDA-MB-231 cells. MTT assay was used to evaluate the viability of MDA-MB-231 cells after treated with AE for 6 h and 24 h. The adhesive potential of MDA-MB-231 cells to FN and LN was tested by cell-matrix adhesion assay. The effect of AE on invasion of MDA-MB-231 cells was measured by Transwell chamber assay. Scratch wound healing assay was applied to determine the effect on migration of MDA-MB-231 cells. The effect of AE on MDA-MB-231 lung metastasis was determined on an experimental metastatic model. 80 micromol/L AE significantly inhibited the invasion, adhesion to FN, LN of MDA-MB-231 cells in vitro, the inhibitory rates were (52.98 +/- 5.46)%, (34.99 +/- 2.63)%, (28.73 +/- 7.00)%, respectively. After 24 h treatment, AE significantly inhibited the migration of MDA-MB-231 cells. The number and volume of lung metastatic nodules formed by MDA-MB-231 cells after 80 micromol/L AE 24 h treatment were decreased compared with control group. AE can suppress the metastasis of MDA-MB-231 cells. Their mechanisms may be related to the inhibition of the capabilities of invasion and migration of MDA-MB-231 cells.

  2. Cardiac troponin I and creatine kinase-MB release after different cardiac surgeries.

    Science.gov (United States)

    Mastro, Florinda; Guida, Pietro; Scrascia, Giuseppe; Rotunno, Crescenzia; Amorese, Lillà; Carrozzo, Alessandro; Capone, Giuseppe; Paparella, Domenico

    2015-06-01

    To conduct a comparative study of cardiac troponin I (cTnI) and MB isoenzyme of serum creatine kinase (CK-MB) after different cardiac surgeries. Consecutive cardiac operations under cardiopulmonary bypass (200 adults, 144 men, 68 ± 11 years): 67 coronary artery bypass graft (CABG), 27 aortic valve surgery, 21 mitral valve surgery, 11 thoracic aorta surgery, and 74 combined surgery. Postoperative cTnI and CK-MB were measured on admission to the ICU and at fixed time until the fifth postoperative day. Peak values of cTnI (median 5.8 ng/ml; interquartile range 3.6-11.9) and CK-MB (29.0 ng/ml; 15.6-60.4) were reached mainly within 18 h after the end of surgery (85% of cTnI and 95% of CK-MB highest determinations) without differences among groups. Cardiopulmonary bypass and cross-clamp time significantly correlated with markers' peak values. At multivariate analysis, mitral valve surgery showed greater cTnI, CK-MB, and their cumulative area under the curve than other isolated procedures. Thoracic aorta surgery showed lower cumulative area under the curve for both markers than CABG and combined surgery. Mitral valve surgery had significant later reduction of both markers in comparison with other procedures. No patient in mitral valve surgery group reached cTnI values in the normal laboratory range within 5 postoperative days. Release pattern of cTnI and CK-MB after heart surgery depends on the type of procedure. Mitral valve surgery was characterized by highest and longest elevation of postoperative markers' concentration. Determinants of differences in myocardial injury biomarkers and their prognostic value after valve surgery should be accurately assessed.

  3. Hypoxia regulates stemness of breast cancer MDA-MB-231 cells.

    Science.gov (United States)

    Xie, Jing; Xiao, Yong; Zhu, Xiao-yan; Ning, Zhou-yu; Xu, Hai-fan; Wu, Hui-min

    2016-05-01

    Human breast cancers include cancer stem cell populations as well as non-tumorigenic cancer cells. Breast cancer stem cells possess self-renewal capability and thus are the root cause of recurrence and metastasis of malignant tumors. Hypoxia is a fundamental pathological feature of solid tumor tissues and exerts a wide range of effects on the biological behavior of cancer cells. However, there is little information on the role of hypoxia in modulating the stemness of breast cancer cells. In the present study, we cultured MDA-MB-231 cells in a hypoxic gas mixture to simulate the hypoxic environment in tissues and to determine how hypoxia conditions could affect the cell proliferation, apoptosis, cytotoxicity, and colony-forming ability. Expression of the stem cell phenotype CD24(-)CD44(+)ESA(+) was analyzed to assess the effects of hypoxia on stemness transformation in MDA-MB-231 cells. Our results found that the cell toxicity of MDA-MB-231 cells was not affected by hypoxia. Hypoxia could slightly inhibit the growth of MDA-MB-231 cells, but the inhibitory effect is not significant when compared with normoxic control. Moreover, hypoxia significantly blocked the apoptosis in MDA-MB-231 cells (P MB-231 cells was increased greatly after they were treated with hypoxia, and cell colony-formation rate of MDA-MB-231 cells also increased significantly in hypoxia-treated cells. These results encourage the exploration of hypoxia as a mechanism which might not be underestimated in chemo-resistant breast cancer treatment.

  4. MB-OFDM UWB系统中交织器盲识别方法%Blind recognition of interleaver in the MB-OFDM UWB system

    Institute of Scientific and Technical Information of China (English)

    张玉; 张伟杰; 杨晓静

    2012-01-01

    借助峰值平均功率比(PAR)与反转误码率(RSER)信息,实现MB-OFDM UWB系统在发送端选择最佳交织器后,在不传送交织辅助信息情况下,在接收端恢复发送端使用的交织器编号,实现交织过程盲识别.将反转误码率的检测应用到对交织器的盲识别中,改进MB-OFDM UWB系统中的交织器与解交织器部分,通过对反转误码率的比较识别出发送端所选择的交织器规格,进而达到对交织序列的盲识别.对常规与改进后的系统进行误比特率仿真比较后,发现改进后的MB-OFDM UWB系统具有较好的可靠性,可以通过反转误码率的比较信息得出交织器编号,进而得到交织序列,释放信道,达到真正意义上的交织盲识别,从而实现智能通信.%A model for blind recognition of interleaver used to recover the interleaving order is presented for MB-OFDM UWB system. After choosing the optimum interleaver with the peak-to-average power (PAR) information, the receiver structure of the improved MB-OFDM UWB system can blindly identify the interleaving order without sending side information. In the improved system, the parts of interleaver and deinterleaver in existing system are enhanced and the reverse symbol error rate (RSER) information is utilized. The rule of the interleaver in transmitter can be estimated without side information by comparing RSER. Simulations show that the system has high reliability in terms of bit error rate compared with the conventional systems. Moreover, the improved MB-OFDM UWB system can release the channel capacity and thus enable intelligent communication.

  5. Regional P-Coda for Stable Estimates of Body Wave Magnitude: Extending the Ms:mb Discriminant to Smaller Events

    Science.gov (United States)

    Mayeda, K.; Bonner, J.

    2007-12-01

    The most successful teleseismic discriminant is Ms:mb, and many studies are underway to try and extend surface wave magnitude (Ms) estimation to regional distances. A problem that is encountered at regional distances and small magnitudes is how to estimate mb so that the Ms:mb discriminant is meaningful and consistent with teleseismic measures. Over the past several years, a regional S-coda wave methodology has been developed that provides for the lowest variance estimate of the seismic source spectrum. Thus, regional MW and mb estimates derived from Sn and Lg coda are very stable, even when only a single station is used. However, these mb's&p are inherently biased for earthquakes because they are an S-based measurement, and explosions are relatively depleted in S-waves. Previous research projects have used region-specific mb scales based on direct measurements of Pn and Pg to improve the Ms:mb discrimination, even though the mb estimates often had a large variance. In our preliminary research, we have found that P-coda envelopes for both explosions and earthquakes can be obtained for events from both the NTS and NZ regions without bias. Our next step at NTS will be to derive path corrections, similar to the approach of Mayeda et al. (2003) for Lg-coda. We will compare inter-station scatter of distance-corrected amplitudes as a function of window length. This will provide an empirical measure of error based on window length for each frequency band. For each frequency band, we will regress our coda envelope amplitudes against regional and teleseismic estimates of mb (e.g., mb(Pn), mb(P)) to determine which band provides the lowest variance. This will yield slope and intercept values for each frequency band. We will then derive mb(Pn) and mb(P) (following Denny et al., 1989) to compare against mb(P-coda) to assess performance at the network and single-station level. Most of the nuclear explosions already have an mb(Pn) compiled by Vergino and Mensing (1989). Patton

  6. The intercalated BSc (Med) Honours/MB ChB and integrated MB ChB/PhD tracks at the University of Cape Town: models for a national medical student research training programme.

    Science.gov (United States)

    Katz, A A; Futter, M; Mayosi, B M

    2014-02-01

    The Faculty of Health Sciences at the University of Cape Town is addressing the shortage of clinician-scientists in South Africa by introducing two research training tracks in parallel with the professional MB ChB programme, namely the intercalated BSc (Med) Hons/MB ChB track and the integrated MB ChB/PhD track. The BSc (Med) Hons/MB ChB track is available to MB ChB students who have completed the first two years of study. The track comprises a course in Molecular Medicine given concurrently with the MB ChB third-year curriculum, followed by a BSc (Med) Hons as a 'year out' of MB ChB. Subsequently students may enroll into the integrated MB ChB/PhD track that enables them to undertake a PhD concurrently with MB ChB studies, which will be spread over additional years, or alternatively to undertake a PhD after completion of the MB ChB. These tracks, which were launched in 2011, represent an opportunity to train a new cadre of young African clinician-scientists at the undergraduate level.

  7. 余额宝对我国商业银行业务的影响及启示%The Influence of Yu’ e Bao on the Businesses of Commercial Banks

    Institute of Scientific and Technical Information of China (English)

    马晓鑫

    2014-01-01

    自2013年余额宝上线之后,在短短一年时间内规模从零发展到近六千亿,对于传统银行业造成了一定的冲击,引起了社会广泛的关注。通过介绍余额宝独特的优势,探讨了余额宝对商业银行存款业务、理财产品和基金代销业务的影响,并提出了重视个体客户、升级理财产品、实现金融产品和服务的多功能化等应对措施。%Since the launch the Yu’ e Bao in 2003,its scale have developed to 600 billion Yuan from zero in only one year and have impacted the traditional commercial banking sector to some extent,which has caused wide atten-tion of the society.This article firstly introduced the unique advantages of Yu’ e Bao,then discussed the influence of Yu’ e Bao on the deposit business,finance products and the business of funds commission sale of the commercial banks,and finally presented relative measures to the banks.

  8. Enhanced photoluminescence properties of Sm3+ ions in Cu+ and Sn2+ co-doped P2O5:BaO glass

    Science.gov (United States)

    Jiménez, José A.

    2014-12-01

    Luminescent glasses activated with Sm3+ ions are of current interest given their potential for a wide range of photonic applications. In this work, Sm3+-containing P2O5:BaO glasses are prepared by a simple melt-quench method, and the influence of CuO and SnO co-doping on Sm3+ photoluminescence (PL) is investigated. Optical absorption, solid-state 31P nuclear magnetic resonance spectroscopy, and PL spectroscopy are employed in the assessment of material optical and structural properties. The data indicates that monovalent copper ions and twofold-coordinated Sn centers are successfully stabilized in the matrix and both species can enhance the orange-red emission of Sm3+ ions. The optical properties of the material after heat treatment have been also assessed. Results indicate the chemical reduction of ionic copper via Sn2+ ultimately producing Cu nanoparticles as evidenced by the surface plasmon resonance. As a result, Sm3+ PL diminishes consistent with an excitation energy transfer to plasmonic Cu particles, i.e. the "plasmonic diluent" effect prevails.

  9. Myth-archetypal Criticism of the Little Bao Village%《小鲍庄》的神话—原型批评解读

    Institute of Scientific and Technical Information of China (English)

    丁琼

    2011-01-01

    This thesis uses myth-archetypal criticism of his theory to reread the "roots" novel of the Little Bao Village,aims to discuss flood myth in the novel replacement deformation,as well as the replacement deformation of human suffering heroes and destroy reconstruction archetypal in the text shift.And the paper analyse in depth righteousness as the core of the traditional culture declining in contemporary society.%本文运用神话—原型批评理论对寻根小说《小鲍庄》进行重读,旨在通过对洪水神话在小说中的置换变形,以及"为人类受难的英雄"和"毁灭—重建"的神话原型在文本中移位的探讨,深入分析以"仁义"为核心的传统文化在当代社会的衰微。

  10. Short-time QiBaoMeiRan Formula Treatment Exerts Estrogenic Activities without Side Effects on Reproductive Tissues in Immature Mice.

    Science.gov (United States)

    Xu, Ying; Ma, Xiao-ping; An, Jin-na; Zhang, Zi-jia; Ding, Jie; Qu, Ya-kun; Liu, Zhen-li; Lin, Na

    2015-12-08

    The Chinese herbal preparation QiBaoMeiRan formula (QBMR) displayed estrogenic effects in ovariectomized rats after long-term administration in a previous study. The uterus and vagina are negatively influenced by estrogens in hormone therapy. While QBMR is known to be a phytoestrogen, its estrogenic effects and safety on reproductive tissues after short-term administration and its mechanism via estrogen receptor (ER) pathway haven't been studied. Here, we characterized its estrogenic effects using immature mice together with in vitro studies for further molecular characterization. Immature mice were treated with QBMR at doses of 1.125, 2.25, and 4.5 g/kg for 7 days. 1.125 and 2.25 g/kg QBMR promoted the growth and development of uterus and vagina, and upregulated ERα and ERβ expression in reproductive tissues. QBMR had a stimulatory effect on proliferating cell nuclear antigen in vagina but not in uterus, and was without any influence on ki-67 antigen in uterus and vagina. QBMR significantly induced luciferase expression from the ERα/β-estrogen response element (ERE) luciferase reporter and upregulated ERα and ERβ expressions in MCF-7 cells, which were significantly inhibited by estrogen antagonist ICI182,780. This study demonstrated QBMR exerts estrogenic effects on reproductive tissues without side effects and through ER-ERE-dependent pathway.

  11. Non-parametric study of the evolution of the cosmological equation of state with SNeIa, BAO and high redshift GRBs

    CERN Document Server

    Postnikov, Sergey; Hernandez, Xavier; Capozziello, Salvatore

    2014-01-01

    We study the dark energy equation of state as a function of redshift in a non-parametric way, without imposing any {\\it a priori} $w(z)$ (ratio of pressure over energy density) functional form. As a check of the method, we test our scheme through the use of synthetic data sets produced from different input cosmological models which have the same relative errors and redshift distribution as the real data. Using the luminosity-time $L_{X}-T_{a}$ correlation for GRB X-ray afterglows (the Dainotti et al. correlation), we are able to utilize GRB sample from the {\\it Swift} satellite as probes of the expansion history of the Universe out to $z \\approx 10$. Within the assumption of a flat FLRW universe and combining SNeIa data with BAO constraints, the resulting maximum likelihood solutions are close to a constant $w=-1$. If one imposes the restriction of a constant $w$, we obtain $w=-0.99 \\pm 0.06$ (consistent with a cosmological constant) with the present day Hubble constant as $H_{0}=70.0 \\pm 0.6$ ${\\rm km} \\, {\\...

  12. Optical spectra and local structure of Eu3+ ions doped in Nb2O5-La2O3-B2O3-BaO glasses

    Institute of Scientific and Technical Information of China (English)

    Haiping Xia; Jianli Zhang; Jinhao Wang; Yuepin Zhang

    2006-01-01

    @@ The xNb2O5-(15-x)La2O3-40B2O3-45BaO (x = 5, 7.5, 12.5 mol%) glasses doped with Eu3+ ions in 1mol% are fabricated by the melting method. The Fourier transform infrared (FTIR) spectra, phonon sideband spectra, emission and excitation spectra of the glasses are measured. The crystal field parameter and coordination number of Eu3+ ions in the glasses are obtained according to the splitting of their 5D0 - 7F1levels. The intensity parameters Ω2 and Ω4 of Eu3+ ions for optical transition are calculated from their emission spectra in terms of reduced matrix U(t) (λ= 2,4,6) character for optical transitions. The results indicate that the intensity parameters Ω2 and Ω4 increase with the increase of Nb2O5 content, suggesting that the symmetry becomes lower, the band of Eu and O atoms becomes stronger and the covalence increases with the increase of Nb2O5 content.

  13. 20-Mb duplication of chromosome 9p in a girl with minimal physical findings and normal IQ: narrowing of the 9p duplication critical region to 6 Mb.

    Science.gov (United States)

    Bonaglia, Maria Clara; Giorda, Roberto; Carrozzo, Romeo; Roncoroni, Maria Elena; Grasso, Rita; Borgatti, Renato; Zuffardi, Orsetta

    2002-10-01

    We studied the case of a girl with a partial 9p duplication, dup(9)(p22.1 --> p13.1). Molecular cytogenetics studies defined the chromosome 9 rearrangement as a direct duplication of 20 Mb from D9S1213 to D9S52. Microsatellite analysis demonstrated the presence of a double dosage of the paternal alleles and demonstrated that the duplication occurred between sister chromatids. The patient's phenotype was almost normal, with a few minor anomalies (dolichocephaly, crowded teeth, high arched palate) and normal IQ. The breakpoint's location in this patient and previously reported cases suggest that the critical region for the 9p duplication syndrome lies within a 6-Mb portion of chromosome 9p22 between markers D9S267 and D9S1213.

  14. Comparison of serum cardiac troponin-I and creatine kinase MB isoenzyme concentrations in asphyxiated neonates

    Institute of Scientific and Technical Information of China (English)

    Nouran F.Hussien; Eman A.Abdel Ghany; Amany E.Elwan; Yasser H.Kamel; Dina K.Ali

    2009-01-01

    Objective:To assess the correlation of signs of myocardial damage to serum cardiac tmponin I(cTnI)and creatine kinase MB isoenzyme(CK-MB)concentrations.Methods:Blood samples were collected from 25 term asphyxiated neonates and 25 controls at 12 h of age by immunoassay.The asphyxiated neonates were followed up until discharge or death.Results:Asphyxiated neonates had significanfly higher concentrations of cTnI and CK-MB than controls(P<0.001).Serum cTnI concentrations were significantly higher in asphyxiated neonates who developed hypotension,heart failure or those had low ejection fraction(P<0.01).Serum cTnI concentrations were significantly higher in asphyxiated who died than those who survived(P<0.01).There was no significant difference in selMnl CK-MB mass concentrations between asphyxiated neonates with and without these complications.Conclusion:Unlike CK-MB,serum cTnI concentrations are significantly higher in asphyxiated neonates who died or developed cardiac dysfunction.

  15. Improving CD uniformity using MB-MDP for 14nm node and beyond

    Science.gov (United States)

    Kim, Byung G.; Choi, Jin; Park, Jisoong; Jeon, Chan U.; Watson, Sterling; Adamov, Anthony; Pack, Bob; Bork, Ingo

    2012-11-01

    Model-Based Mask Data Preparation (MB-MDP) has been discussed in the literature for its benefits in reducing mask write times [1][2]. By being model based (i.e., simulation based), overlapping shots, per-shot dose modulation, and circular and other character projection shots are enabled. This reduces variable shaped beam (VSB) shot count for complex mask shapes, and particularly ideal ILT shapes [3]. In this paper, the authors discuss another even more important aspect of MB-MDP. MB-MDP enhances CD Uniformity (CDU) on the mask, and therefore on the wafer. Mask CDU is improved for sub-80nm features on mask through the natural increase in dose that overlapping provides, and through per-shot dose modulation. The improvement in CDU is at the cost of some write times for the less complex EUV masks with only rectangular features. But these masks do not have the basis of large write times that come from complex SRAFs. For ArF masks for the critical layers at the 20nm logic node and below, complex SRAFs are unavoidable. For these shapes, MB-MDP enhances CDU while simultaneously reducing write times. Simulated and measured comparison of conventional methodology and MB-MDP methodology are presented.

  16. MB-SWIFT functional MRI during deep brain stimulation in rats.

    Science.gov (United States)

    Lehto, Lauri J; Idiyatullin, Djaudat; Zhang, Jinjin; Utecht, Lynn; Adriany, Gregor; Garwood, Michael; Gröhn, Olli; Michaeli, Shalom; Mangia, Silvia

    2017-08-07

    Recently introduced 3D radial MRI pulse sequence entitled Multi-Band SWeep Imaging with Fourier Transformation (MB-SWIFT) having virtually zero acquisition delay was used to obtain functional MRI (fMRI) contrast in rat's brain at 9.4 T during deep brain stimulation (DBS). The results demonstrate that MB-SWIFT allows functional images free of susceptibility artifacts, and provides an excellent fMRI activation contrast in the brain. Flip angle dependence of the MB-SWIFT fMRI signal and elimination of the fMRI contrast while using saturation bands, indicate a blood flow origin of the observed fMRI contrast. MB-SWIFT fMRI modality permits activation studies in the close proximity to an implanted lead, which is not possible to achieve with conventionally used gradient echo and spin echo - echo planar imaging fMRI techniques. We conclude that MB-SWIFT fMRI is a powerful imaging modality for investigations of functional responses during DBS. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.

  17. Fuel loading experiments of IPEN/MB-01 reactor core; Experimento de carregamento do nucleo do reator IPEN/MB-01

    Energy Technology Data Exchange (ETDEWEB)

    Abe, Alfredo; Angioletto, Elcio; Pasqualetto, Hertz; Moreira, Joao M.L.; Sabo, Marcos A.; Fuga, Rinaldo [Centro Tecnologico da Marinha em Sao Paulo (CTMSP), SP (Brazil). E-mail: ayabe@net.ipen.br; Jerez, Rogerio [Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil)

    2000-07-01

    IPEN/MB-01 is a critical assembly which reaches a first critically at November 1998, since then a several experiments have been conducted in order to validate and qualify the reactor Physics methodologies and codes. Recently, loading experiment was performed to determine the critical number of fuels rods using a multiplication inverse technique (1/M). The experiment can be considered as benchmark and contribute toward reactor physics methodologies, codes and basic nuclear data libraries validation qualification. (author)

  18. Precautions for Providing 2 Mb/s Channel for TDCS%为TDCS提供2Mb/s通道的注意事项

    Institute of Scientific and Technical Information of China (English)

    李珂

    2006-01-01

      为使铁路运输向信息化迈进,沈阳铁路局将大面积开通TDCS(列车调度指挥系统).根据以往对沈山线TDCS的维护经验,在提供2Mb/s通道时应注意以下问题.

  19. The Cambridge Bachelor of Medicine (MB)/Doctor of Philosophy (PhD): graduate outcomes of the first MB/PhD programme in the UK.

    Science.gov (United States)

    Cox, Timothy M; Brimicombe, James; Wood, Diana F; Peters, D Keith

    2012-12-01

    We reviewed outcomes of the Cambridge Bachelor of Medicine (MB)/Doctor of Philosophy (PhD) programme for the period 1989-2010. Of the 90 alumni contacted, 80 (89%; 24 women) completed an anonymous questionnaire. Thirty were academic staff and 35 were in general professional (core) or higher medical training. Of the latter, 11 were specialty registrars, six were academic clinical fellows and three held academic foundation year posts. Eight alumni were overseas, including five in North America. Most (95%) respondents considered that their academic career goals were facilitated by the programme. Sixty-eight of the 80 alumni had conducted further research, 63 (79%) were active in research, and 90% had explicit plans for further full-time research. Twelve graduates had further substantive research support (six clinician scientist awards and three senior fellowships) and two were Wellcome Trust postdoctoral MB/PhD fellows. Alumni included two full university professors, one reader, six senior lecturers, two assistant professors and nine university clinical lecturers. MB/PhD programmes offer an alternative training pathway for clinician-scientists in UK medical schools: the Cambridge programme promotes scientific discovery and sustained academic development within the context of contemporary medicine and clinical practice.

  20. Effect of amlodipine on apoptosis of human breast carcinoma MDA-MB-231 cells

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Objective: To elucidate the effects of amlodipine on the proliferation and apoptosis of human breast carcinoma MDA-MB-231 cells. Methods: Light microscopy was used to determine the effects of amiodipine on cell morphology; Flow cytometry was used to quantitate cells undergoing apoptosis; the expression of a cell cycle-related protein, proliferating cell nuclear antigen (PCNA) and an antiapoptosis protein, Bcl-2 were assessed by immunocytochemistry. Results: Amlodipine concentration of 8.25 Ixmol/L (1/2 of IC50) affected the morphology, decreased the expression of PCNA and Bcl-2 and induced apoptosis of human breast carcinoma MDA-MB-231 cells. Conclusion: The effect of amlodipine on the antiproliferation of human breast carcinoma MDA-MB-231 cells is related to inducement of apoptosis, and the decrease of the expression of Bcl-2 and PCNA may be the possible mechanism for proliferation inhibitory and inducement of apoptosis.

  1. Serum dosage of CPK-MB in dogs with ST deviation by chemiluminescence

    Directory of Open Access Journals (Sweden)

    André L.F. Santos

    2014-12-01

    Full Text Available Abstract: Although frequently in humans, hypoxic and ischemic heart diseases are poorly documented in dogs, with only few reports of acute myocardial infarction (AMI in this species. Some electrocardiographic findings might suggest myocardium hypoxia/ischemia, like ST segment elevation or depression, but there are no studies showing whether deviations in ST segment are associated to myocardial injury and serum increase of creatine phosphokinase (CPK-MB. In order to investigate possible myocardial cells injury in poor perfusion conditions, 38 dogs were studied, 20 with normal electrocardiogram and 18 with ST segment elevation or depression, recorded in lead II, at a paper speed of 50 mm/sec and N sensibility (1mV=1cm. Serum measurement of creatine phosphokinase isoenzyme MB (CPK-MB in normal dogs (group 1 determined control values (in ng/mL, which were compared to those obtained from dogs with deviation (group 2, which allowed confirmation or not of myocardial injury. CPK-MB mean values obtained from dogs in groups 1 and 2 were 0.540ng/ml (SD±0.890ng/mL and 0.440ng/mL (SD±1.106, respectively. At a significance level of 5%, the relation of CPK-MB with age, mass and total creatine phosphokinase (CPK-T was not significant in groups 1 and 2. CPK-MB showed no difference, at 5% level, between groups 1 and 2. In conclusion, it is possible to use the human chemiluminescent immunometric assay kit in canine species and that hypoxia/ischemia revealed by ST segment deviation does not mean significant myocardium injury.

  2. Gender differences in serum CK-MB mass levels in healthy Braziliansubjects

    Directory of Open Access Journals (Sweden)

    C.M.C. Strunz

    2011-03-01

    Full Text Available The creatine kinase-isoenzyme MB (CK-MB mass assay is one of the laboratory tests used for the diagnosis of myocardial infarction. It is recommended, however, that reference limits should take gender and race into account. In the present study, we analyzed the plasma CK-MB mass and troponin levels of 244 healthy volunteers without a personal history of coronary artery disease and with no chronic diseases, muscular trauma or hypothyroidism, and not taking statins. The tests were performed with commercial kits, CK-MB mass turbo kit and Troponin I turbo kit, using the Immulite 1000 analyzer from Siemens Healthcare Diagnostic. The values were separated according to gender and showed significant differences by the Mann-Whitney test. Mean (± SD CK-MB mass values were 2.55 ± 1.09 for women (N = 121; age = 41.20 ± 10.13 years and 3.49 ± 1.41 ng/mL for men (N = 123; age = 38.16 ± 11.12 years. Gender-specific reference values at the 99th percentile level, according to the Medicalc statistical software, were 5.40 ng/mL for women and 7.13 ng/mL for men. The influence of race was not considered because of the high miscegenation of the Brazilian population. The CK-MB values obtained were higher than the 5.10 mg/mL proposed by the manufacturer of the laboratory kit. Therefore, decision limits should be related to population and gender in order to improve the specificity of this diagnostic tool, avoiding misclassification of patients

  3. Protein Profiles Associated with Anoikis Resistance of Metastatic MDA-MB-231 Breast Cancer Cells.

    Science.gov (United States)

    Akekawatchai, Chareeporn; Roytrakul, Sittiruk; Kittisenachai, Suthathip; Isarankura-Na-Ayudhya, Patcharee; Jitrapakdee, Sarawut

    2016-01-01

    Resistance to anoikis, a cell-detachment induced apoptosis, is one of the malignant phenotypes which support tumor metastasis. Molecular mechanisms underlying the establishment of this phenotype require further investigation. This study aimed at exploring protein expression profiles associated with anoikis resistance of a metastatic breast cancer cell. Cell survival of suspension cultures of non-metastatic MCF-7 and metastatic MDA-MB-231 cells were compared with their adherent cultures. Trypan blue exclusion assays demonstrated a significantly higher percentage of viable cells in MDA-MB-231 than MCF-7 cell cultures, consistent with analysis of annexin V-7-AAD stained cells indicating that MDA-MB-231 possess anti-apoptotic ability 1.7 fold higher than MCF-7 cells. GeLC-MS/MS analysis of protein lysates of MDA-MB-231 and MCF-7 cells grown under both culture conditions identified 925 proteins which are differentially expressed, 54 of which were expressed only in suspended and adherent MDA-MB-231 but not in MCF-7 cells. These proteins have been implicated in various cellular processes, including DNA replication and repair, transcription, translation, protein modification, cytoskeleton, transport and cell signaling. Analysis based on the STITCH database predicted the interaction of phospholipases, PLC and PLD, and 14-3-3 beta/alpha, YWHAB, with the intrinsic and extrinsic apoptotic signaling network, suggesting putative roles in controlling anti-anoikis ability. MDA-MB-231 cells grown in the presence of inhibitors of phospholipase C, U73122, and phospholipase D, FIPI, demonstrated reduced ability to survive in suspension culture, indicating functional roles of PLC and PLD in the process of anti-anoikis. Our study identified intracellular mediators potentially associated with establishment of anoikis resistance of metastatic cells. These proteins require further clarification as prognostic and therapeutic targets for advanced breast cancer.

  4. Development of FEC and ESC performance characteristics for IDR carriers greater than 10 Mb/s

    Science.gov (United States)

    Loureiro, Francisco; Koga, Keiichiro

    1988-12-01

    The requirement for restoration of high capacity fiber optic cables and the new tariffs approved for Intelsat digital services has created a requirement to develop performance characteristics for intermediate data rate (IDR) carriers greater than 10 Mb/s. This paper presents the trade-offs used in this development, and estimates the coding gains needed to meet these requirements. A number of forward error correction schemes are evaluated and a rate 3/4 convolutional code with soft decision Viterbi decoding is selected. Engineering service circuits performance characteristics for IDR carriers greater than 10 Mb/s are presented.

  5. [The concept of madness among the Cainguá (Mbŷá) of Misiones].

    Science.gov (United States)

    Pagés Larraya, F

    1985-06-01

    To analyse the madness concept among the Cainguá (Mbŷá) it is necessary to be acquainted with their culture, as this concept becomes meaningful only within that culture frame. The Cainguá (Mbŷá) have preserved a singular theology whereof the comprehension makes possible an approach to the real being within that culture. This group keeps its ethnic individuality through a culturally patterned attitude of rejection and withdrawal from accidental culture, which has been dominant in its territory, especially since the 17th century.

  6. STS-26 crew during MB shuttle mission simulator (SMS) training in Bldg 5

    Science.gov (United States)

    1987-01-01

    STS-26 Discovery, Orbiter Vehicle (OV) 103, crewmembers participate in motion base (MB) shuttle mission simulator (SMS) training in JSC Mission Simulation and Training Facility Bldg 5. Five veteran astronauts recently named to man the Discovery for the STS-26 mission are pictured prior to a training session in the nearby MB SMS. Commander Frederick H. Hauck (top right) stands next to Pilot Richard O. Covey with (left to right) Mission Specialist (MS) George D. Nelson, MS David C. Hilmers, and MS John M. Lounge on front row.

  7. Clinical and financial impact of removing creatine kinase-MB from the routine testing menu in the emergency setting.

    Science.gov (United States)

    Le, Rachel D; Kosowsky, Joshua M; Landman, Adam B; Bixho, Ida; Melanson, Stacy E F; Tanasijevic, Milenko J

    2015-01-01

    Cardiac troponins T and I have replaced creatine kinase-MB (CK-MB) as the criterion standard for diagnosing myocardial injury. However, many laboratories still routinely perform a high volume of CK-MB testing in conjunction with troponin. The purpose of this study is to study the clinical and financial impact of removing CK-MB from the routine emergency department (ED) test menu at a large academic medical center. Creatine kinase-MB was removed from ED ordering templates and laboratory requisitions (ie, intervention), although the test could still be manually ordered. Data for creatine kinase (CK), CK-MB, and troponin T (TnT) specimens ordered during a 12-month period (6 months preintervention and 6 months postintervention) (n = 14571) was downloaded from our laboratory information system. All specimens with (1) normal TnT (ie, 6.6 ng/mL), and (3) elevated CK-MB index (ie, >5) were considered discrepant and independently reviewed by 2 ED clinicians for the presence of an acute coronary syndrome and for documentation of final diagnosis. Creatine kinase, CK-MB, and TnT ED volumes preintervention and postintervention were analyzed to assess laboratory cost savings. Of the 6444 cases included in the analysis, only 17 were discrepant. Of all 17 cases, no patients were diagnosed with acute coronary syndrome. After removing CK-MB from the templates and requisitions, CK-MB and CK volumes decreased by 80% and 76%, respectively, translating to annual reagent cost savings of approximately $47000. Creatine kinase-MB can be removed from the routine ED test menu without adversely affecting patient care. In addition, substantial cost savings can be achieved by reducing unnecessary CK-MB testing and associated CK orders. Copyright © 2014 Elsevier Inc. All rights reserved.

  8. Characterization of a functional recombinant human creatine kinase-MB isoenzyme prepared by tandem affinity purification from Escherichia coli.

    Science.gov (United States)

    Zou, Lihui; Su, Wen; Wang, Meng; Huang, Wei; Zhao, Haijian; Zhang, Enyi; Jin, Junhua; Xu, Hongtao; Xiao, Fei

    2017-07-01

    Creatine kinase isoform CK-MB has been widely applied as a biomarker of myocardial injury. While a variety of methods have been used to measure CK-MB activity or mass in clinical laboratories, a CK-MB standard is needed to eliminate between-method bias. Because the in vitro expression of human creatine kinase generates three isoenzymes, CK-MM, CK-MB, and CK-BB, it is important to establish an effective method to purify the isoform CK-MB from the mixture. In this study, we aimed at using tandem affinity purification (TAP) to purify recombinant CK-MB protein and evaluate its value in clinical laboratories. After the optimized sequence coding CK-M and CK-B were synthesized, they were combined with TAP tags (6His and SBP) and inserted into a pRSFDuet vector; then, the constructed 6His-CK-M-SBP-CK-B-pRSF plasmid was transformed into Escherichia coli BL21 (DE3) for expression. After TAP, we obtained purified CK-MB protein. We also did recovery testing using the engineered CK-MB and standard CK-MB (Randox) at different concentrations, and the results suggested that the engineered CK-MB could be used as the reference material. Moreover, the stability study of recombinant CK-MB showed high stability during long-term storage at -80 °C. In conclusion, the TAP-purified recombinant CK-MB protein may be a much better and cheaper standard or reference material for clinical laboratories.

  9. Creatinine kinase isoenzyme-MB: A simple prognostic biomarker in patients with pulmonary embolism treated with thrombolytic therapy.

    Science.gov (United States)

    Bozbay, Mehmet; Uyarel, Huseyin; Avsar, Sahin; Oz, Ahmet; Keskin, Muhammed; Tanik, Veysel Ozan; Bakhshaliyev, Nijat; Ugur, Murat; Pehlivanoglu, Seckin; Eren, Mehmet

    2015-12-01

    Creatinine kinase isoenzyme-MB (CK-MB) is a biomarker for detecting myocardial injury. The aim of this study was to evaluate the association between admission CK-MB levels and in-hospital and long-term clinical outcomes in pulmonary embolism (PE) patients treated with thrombolytic tissue-plasminogen activator. A total of 148 acute PE patients treated with tissue-plasminogen activator enrolled in the study. The study population was divided into 2 tertiles, based on admission CK-MB levels. The high CK-MB group (n=35) was defined as having a CK-MB level in the third tertile (>31.5 U/L), and the low group (n=113) was defined as having a level in the lower 2 tertiles (≤31.5 U/L). High CK-MB group had a higher incidence of in-hospital mortality (37.1% vs 1.7%, PMB group. In the receiver-operating characteristic curve analysis, a CK-MB value of more than 31.5 U/L yielded a sensitivity of 86.7% and specificity of 83.5% for predicting in-hospital mortality. During long-term follow-up, recurrent PE, major and minor bleeding, and mortality rates were similar in both groups. Creatinine kinase isoenzyme-MB is a simple, widely available, and useful biomarker for predicting adverse in-hospital clinical outcomes in PE. Copyright © 2015 Elsevier Inc. All rights reserved.

  10. Implementation of a programme to market a complementary food supplement (Ying Yang Bao) and impacts on anaemia and feeding practices in Shanxi, China.

    Science.gov (United States)

    Sun, Jing; Dai, Yaohua; Zhang, Shuaiming; Huang, Jian; Yang, Zhenyu; Huo, Junsheng; Chen, Chunming

    2011-10-01

    In China, a full fat soy powder mixed with multiple micronutrient powders (Ying Yang Bao (YYB)) was developed, and the efficacy of YYB was shown in controlling anaemia and improving child growth and development. However, prior to 2008, there was no sustainable way to provide YYB to vulnerable populations, except through free distribution by the government. This study was to test the concept of public-private partnership (PPP) to deliver YYB and to evaluate the effectiveness of marketing YYB through PPP. Programme activities included development of a complementary food supplement (CFS) national standard, product concept test, product development and marketing, behavior change communication, monitoring and evaluation. Baseline and end-line surveys were used to evaluate product awareness, purchasing and the impacts of the project on anaemia and feeding practices. A Chinese CFS standard was approved. Caregivers and their 6- to-24-month-old children participated in the baseline (n=226) and the end-line survey (n=221). A concept test at the baseline survey showed that 78% of caregivers were willing to buy YYB at 0.1 USD. After developing the product and implementing the intervention for 8 months, 59.6% of surveyed caregivers purchased YYB. While not significant, the prevalence of anaemia was marginally lower at the end line (28.8%) than at the baseline (36.2%). For those purchasing YYB, the risk of anaemia was significantly reduced by 87% of odds (Pmarketing are identified as a prerequisite for marketing YYB or other nutritious CFS. Public and private advocacy and marketing could successfully increase awareness of YYB and access and use through market channels. The YYB project may be effective for reducing anaemia and improving feeding practices.

  11. Study on the Music Materials of Shang Qing Ling Bao Dafa in the Southern Song Dynasty%唐代伎乐的守成与新变

    Institute of Scientific and Technical Information of China (English)

    夏滟洲

    2016-01-01

    This paper gave a detailed interpretation of ritual music document Shang QingLingBaoDafa in the Southern Song Dynasty. And especially focusing on the rit-ual music of Huangluzhai, Chuanduyi, which involving the evolution of Lingbaozhai music overview from ancient to nowadays. It points out that in the integration and transformation of the old and new rituals, the stylish plot ritual model have the upper hand, and would become the main trend to developing the ritual music in the future. Thus it has a glimpse the characteristics and outline of development of ritual music in the Southern Song Dynasty. It also analyzed thorough the ritual music related to liter-ature such as important concepts, the phenomenon and logic connotation of the ritual.%唐代宫廷音乐中,多部伎的形成与运用,有其深刻的政治社会背景。从唐初对九部乐的改订到十部乐之用,再到二部伎的增造,充分地说明了这一点。随着唐代将散乐列于乐部,隐藏于多部伎创制中的因素被激发出来,从而形成了唐代多样化、娱乐化的伎乐体系,促进了诸多新的、普世性、社会性音乐品类流行,中古音乐雅俗之分明确,中古伎乐演化初步实现。

  12. Study on Movie Advertisements on Shen Bao from 1890 to 1891%1890-1891年《申报》影戏广告研究

    Institute of Scientific and Technical Information of China (English)

    孙慧

    2016-01-01

    对《申报》广告进行检视,不难发现,同时期的商品广告,已呈现出分阶段的广告策略,而戏曲广告,尤其是夹杂在戏曲广告中的电影广告,不仅广告文案的差异性很小,且广告策略的制定也慢了许多。整体而言,1890年没有任何影戏广告,1891年出现了3个广告主、7条影戏广告和12条疑似影戏广告的投放,全年影戏广告呈现“一月纠缠,九月孤单”的特征。研究这一时期的电影广告,将帮助我们重新认识处于电影发生期的1890年至1891年,电影传入中国的过程。%If we examine advertisements printed on Shen Bao ( Shanghai News ) , it is not difficult for us to find that contemporaneous product advertisements had already presented a staged advertising strategy, but advertisements of traditional Chinese opera , especially movie advertisements that were mixed up with opera advertisements , had shown very small differences in advertising copy , and the formulation of advertising strategies was also much slower .On the whole , no movie advertisements appeared in 1890; there were three advertisers in 1891 , with seven movie advertisements and 12doubtable movie advertisements were released .By studying movie advertisements during this period , we re-understand the process of movies ’ introduction to China .

  13. ON THE PAR REDUCTION OF MB-OFDM ULTRA-WIDEBAND SIGNALS

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    The Peak to Average power Ratio (PAR) of a Multi-Band Orthogonal Frequency-Division Multi-plexing (MB-OFDM) Ultra-Wide Band (UWB) signals can be substantially larger than that of single carrieror carrier-less ultra-wideband signals. In this letter, a novel PAR reduction scheme for the MB-OFDM UWBsystem based on spreading and interleaving is proposed. By spreading the coded bits over each subcarrier incorresponding band and interleaving the spread symbols across all bands, the PAR statistics of theMB-OFDM signals can be improved and the PAR is reduced obviously. In the PAR reduction scheme, there isno loss in transmission data rate or Bit Error Rate (BER) performance decreasing. Since the spreading andinterleaving operation are implemented by unitary Hadamard sequences and used for an approach to providethe robustness of the UWB system to narrowband interference, there is no additional implementation burden.Simulation results show that the investigated scheme gives the PAR reduction of 3dB compared with that ofthe original MB-OFDM signals.

  14. MB隐写分析算法%A Steganalysis Algorithm on MB Steganography

    Institute of Scientific and Technical Information of China (English)

    董秀则; 张茹; 钮心忻

    2012-01-01

    对图像的高阶统计量进行研究,首先利用离散余弦变换(DCT)域共生矩阵、空域边界梯度能量和空域图像子块的虚特征值分解等对基于模型(MB)隐写带来的变化进行描述,然后利用支持向量机( SVM)设计了MB隐写分析算法.实验结果表明,算法在保持低虚警率的同时具有较高的检出率.%A new steganalysis algorithm on model-based (MB) steganography is proposed after researching the high order statistics of images. The difference, imported fay MB steganography is described by the co-occurrence discrete cosine transform(DCT) matrix, the boundary gradient energy in airspace and the imaginary eigenvalue decomposition. The MB steganography is detected using all of the features and support vector machine. Experiments show that this algorithm has a high accuracy rate of detection with a low false detection rate.

  15. Genome Sequence of Klebsiella quasipneumoniae subsp. similipneumoniae MB373, an Effective Bioremediator

    Science.gov (United States)

    Aslam, Fozia; Thomas, Torsten

    2016-01-01

    Klebsiella quasipneumoniae subsp. similipneumoniae MB373 was isolated from effluent of the Hattar Industrial Estate, Haripur, Pakistan. K. quasipneumoniae subsp. similipneumoniae has few cultivated/characterized members so far. Whole-genome sequencing revealed its potential for metal and toxin resistance, which further elucidated various enzymatic processes for the degradation of xenobiotics, illuminating its bioremediation applications. PMID:27688323

  16. Ovicidal activity of Metarhizium brunneum (Mb F52) on dengue fever vector, Aedes aegypti

    Science.gov (United States)

    The ovicidal activity of Metarhizium brunneum F52 (Mb F52) grown from granules was evaluated against Aedes aegypti eggs over time. Survival of larvae from treated eggs was significantly less when compared with untreated eggs at 7, 10 and 14 days post treatment. Only 27 % of treated eggs produced vi...

  17. Melt Rate Improvement for DWPF MB3: Foaming Theory and Mitigation Techniques

    Energy Technology Data Exchange (ETDEWEB)

    Peeler, D.K.

    2001-07-24

    The objective of this research is to enhance the basic understanding of the role of glass chemistry, including the chemical kinetics of pre-melting, solid state reactions, batch melting, and the reaction pathways in glass and/or acid addition strategy changes on the overall melting process for the Defense Waste Processing Facility (DWPF) Macrobatch 3 (MB3).

  18. Arrival at CERN of the first MB4 DT chambers from Turin

    CERN Multimedia

    Marcos Fernandez Garcia

    2005-01-01

    The largest DT chambers to be installed into the outermost station of the CMS barrel muon detector (MB4) are built by INFN-Turin. The first batch of four chambers, out of 40, arrived at the CERN ISR area on February 2005.

  19. Preliminary Study of m(b) Bias at Selected Soviet Seismic Stations.

    Science.gov (United States)

    1986-03-21

    8217-R66 395 PRELIMINARY STUDY OF M(B) BIRS AT SELECTED SOVIET 1/1 SEISMIC STRTIONS(U) SCIENCE APPLICATIONS INTERNATIONAL CORP ARLINGTON VR A S RYRLL...earthquakes in each source region was not given in the ,.t 1980 paper, but in the earlier work it ranged from 35 events for Asia and the Mediter - ranean to

  20. Electrochemical impedance immunosensor for the detection of cardiac biomarker Myogobin (Mb) in aqueous solution

    Energy Technology Data Exchange (ETDEWEB)

    Rajesh, E-mail: rajesh_csir@yahoo.com; Sharma, V.; Tanwar, V.K.; Mishra, S.K.; Biradar, A.M.

    2010-11-30

    A label-free, electrochemical impedance immunosensor based on surface modified thin flat gold wire electrode is reported for the quantitative detection of cardiac biomarker Myoglobin in aqueous solution. The protein antibody, ab-Mb, was covalently immobilized through a self assembled monolayer of 11-mercaptoundecanoic acid (MUA) and 3-mercapto propionic acid (MPA) via carbodiimide coupling reaction using N-(3-dimethylaminopropyl)-N'-ethyl carbodiimide hydrochloride (EDC) and N-Hydroxy Succinamide (NHS). The immunosensor (ab-Mb/MUA-MPA/Au) was characterized by electrochemical techniques. The electrochemical performance of the immunosensor was studied by electrochemical impedance spectroscopy. The immunosensor showed an increased electrontransfer resistance on coupling with biomarker protein antigen, ag-Mb, in the presence of a redox probe [Fe (CN){sub 6}]{sup 3-/4-}. The modified Au electrode immunosensor exhibits an electrochemical impedance response to antigen, ag-Mb concentrations in a linear range from 10 ng to 650 ng mL{sup -1} with a lowest detection limit of 5.2 ng mL{sup -1}.

  1. Clinical importance of elevated CK-MB and troponin I levels in congestive heart failure.

    Science.gov (United States)

    Yilmaz, Ahmet; Yalta, Kenan; Turgut, Okan Onur; Yilmaz, Mehmet Birhan; Ozyol, Ali; Kendirlioglu, Omer; Karadas, Filiz; Tandogan, Izzet

    2006-01-01

    Myocyte necrosis has been considered to play a fundamental role in the pathophysiology of congestive heart failure (CHF), which has usually evolved as a consequence of depletion of compensatory mechanisms and contractile reserve of myocardium. Elevated levels of creatine kinase MB (CK-MB) and troponin I (Tn-I) have been regarded as biochemical markers of myocyte necrosis. This study was planned to investigate the specificity and sensitivity of Tn-I and CK-MB in CHF and to examine the correlation of these markers with disease severity. A total of 104 patients (38 female, 66 male; mean age, 66 y [range, 36-89]) with symptoms and signs of heart failure on admission and with a reduced left ventricular ejection fraction (EF; by transthoracic echocardiography) were labeled "the patient group," and 58 patients (40 female,18 male; mean age, 61 y [range, 34-77]) with no signs or symptoms of CHF and with a normal EF detected by transthoracic echocardiography were included in the study as "the control group." Left ventricular EFs, end-diastolic diameters, and end-systolic diameters of patients in both groups were measured. Blood samples were drawn from all patients in both groups on admission, so that levels of CK-MB and Tn-I could be measured. All patients in both groups also underwent coronary angiography. Conditions leading to elevation of CK-MB or Tn-I were considered exclusion criteria. The 2 groups failed to show any significant differences in terms of mean age and the presence of coronary artery disease, hypertension, or diabetes mellitus (P>.05). Mean EF in the patient group was lower than that in the control group (Phypertensive patients were found to have significantly higher mean values of CK-MB than were seen in normotensive patients in the same group (PHeart Association [NYHA]) (group 1), and 52 were considered to be class III-IV (group 2). Group 1, group 2, and the control group did not differ significantly from one another with regard to the presence of

  2. Multicenter evaluation of the MB/BACT system for susceptibility testing of Mycobacterium tuberculosis.

    Science.gov (United States)

    Bemer, Pascale; Bodmer, Thomas; Munzinger, Juerg; Perrin, Monique; Vincent, Véronique; Drugeon, Henri

    2004-03-01

    The reliability of the MB/BACT system for susceptibility testing of Mycobacterium tuberculosis to pyrazinamide, rifampin, isoniazid, streptomycin, and ethambutol was compared to the BACTEC 460TB system. The proportion method was used to resolve discrepant results by an independent arbiter. Two interpretative methods were used, with an undiluted control (direct control) and a diluted control (10(-1) control). As no significant difference was observed between the two controls, the method with the direct control was adopted as the most accurate one. One hundred sixty-six strains were tested, with an overall agreement of 98.3%. After resolution of the 18 discrepant results by the proportion method, the sensitivity and specificity of the MB/BACT system were 100% for rifampin, isoniazid, and pyrazinamide. For ethambutol, sensitivity was 92.3% at the critical concentration and 33% at the high concentration, and specificity was 100% at both concentrations. For streptomycin, sensitivity was 100% at the critical concentration and 80% at the high concentration, and specificity was 98.6% at the critical concentration and 100% at the high concentration. The rifampin, isoniazid, streptomycin, and ethambutol susceptibility test results were obtained in 6.6 days with the MB/BACT versus 5 days with the BACTEC 460TB. The pyrazinamide susceptibility test results were obtained in 7.8 days with the MB/BACT, versus 6.7 days with the BACTEC 460TB. These data demonstrate that the fully automated MB/BACT system is a very reliable method for rapid susceptibility testing of M. tuberculosis against rifampin, isoniazid, and pyrazinamide. Sensitivity results have to be improved for ethambutol and streptomycin, especially at the high concentration.

  3. Improved protein quality in transgenic soybean expressing a de novo synthetic protein, MB-16.

    Science.gov (United States)

    Zhang, Yunfang; Schernthaner, Johann; Labbé, Natalie; Hefford, Mary A; Zhao, Jiping; Simmonds, Daina H

    2014-06-01

    To improve soybean [Glycine max (L.) Merrill] seed nutritional quality, a synthetic gene, MB-16 was introduced into the soybean genome to boost seed methionine content. MB-16, an 11 kDa de novo protein enriched in the essential amino acids (EAAs) methionine, threonine, lysine and leucine, was originally developed for expression in rumen bacteria. For efficient seed expression, constructs were designed using the soybean codon bias, with and without the KDEL ER retention sequence, and β-conglycinin or cruciferin seed specific protein storage promoters. Homozygous lines, with single locus integrations, were identified for several transgenic events. Transgene transmission and MB-16 protein expression were confirmed to the T5 and T7 generations, respectively. Quantitative RT-PCR analysis of developing seed showed that the transcript peaked in growing seed, 5-6 mm long, remained at this peak level to the full-sized green seed and then was significantly reduced in maturing yellow seed. Transformed events carrying constructs with the rumen bacteria codon preference showed the same transcription pattern as those with the soybean codon preference, but the transcript levels were lower at each developmental stage. MB-16 protein levels, as determined by immunoblots, were highest in full-sized green seed but the protein virtually disappeared in mature seed. However, amino acid analysis of mature seed, in the best transgenic line, showed a significant increase of 16.2 and 65.9 % in methionine and cysteine, respectively, as compared to the parent. This indicates that MB-16 elevated the sulfur amino acids, improved the EAA seed profile and confirms that a de novo synthetic gene can enhance the nutritional quality of soybean.

  4. Normal values of creatine kinase and of MB-creatine kinase at birth in healthy babies.

    Science.gov (United States)

    Bellieni, Carlo V; Tomasini, Barbara; Bracciali, Carlotta; Buonocore, Giuseppe

    2017-04-20

    Today, few studies have been accomplished in order to determine serum creatine kinase (CK) activity in newborns by considering small groups of babies and without taking into account gestational age (GA) differences. Some authors have demonstrated that neonatal CK activity value at birth is higher than the normal range of CK activity considering for adults or older children. The objective of this study is to assess normal values of CK and MB-CK in neonatal blood, according to babies' GA. We retrieved the clinical files of 140 babies admitted into Siena Hospital NICU in a 2-years period, when CK was assessed routinely to all babies at birth. We selected files from 114 newborns and we divided the cohort into group A (non-stressed; n=41) and group B (stressed; n=73) on the basis of Apgar score and signs of neurological lesions. We compared CK and MB-CK values in the two groups according to GA. Mean CK value of the 41 non-stressed babies' samples: 413 IU/L (232 SD). CK significantly increases with GA. No differences are present in total CK activity between stressed vs non-stressed babies; but a significant difference appears in these two groups for MB-CK (mean values: 456 vs 175 IU/L). This is the first study that compares CK and MB-CK values at birth according to the GA of the babies. CK values increase with GA, and stressed babies have higher MB-CK values than the non-stressed babies. These reference values are important for clinical practice.

  5. M@B9 and M@B10 molecular wheels containing planar nona-and deca-coordinate heavy group 11, 12, and 13 metals (M=Ag, Au, Cd, Hg, In, Tl)

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    A density functional and ab initio theory investigation on M@B9 and M@B10 molecular wheels containing planar nona-and deca-coordinate heavy group 11, 12 and 13 metals (M=Ag, Au, Cd, Hg, In, Ti) has been performed. These unusual clusters all prove to be true minima on the potential energy surfaces of the systems and σ+π double aromatic in nature. The first two vertical one-electron detachment energies of M@B-10 (M=Ag, Au) anions and first two ionization potentials of M@B9 (M=Ag, Au) and M@B10 (M=Cd, Hg) neutrals were calculated to aid future experiments. The cluster complexes designed in this work may expend the domain of planar hyper-coordinate elements to include heavy group 11, 12, and 13 metals and serve as interesting candidates to be targeted in experiments.

  6. 3TiO_2-45.7BaO-51.3B_2O_3微晶玻璃的SHG%Second Harmonic Generation of the 3TiO_2-45.7BaO-51.3B_2O_3 Transparent Crystallized Glasses

    Institute of Scientific and Technical Information of China (English)

    曹艳丽; 陈静; 顾少轩; 郑玲江

    2009-01-01

    The crystallized glasses of 3TiO_2-45.7BaO-51.3B_2O_3 system were prepared by heat treatment method.The resuits of XRD and transmittance spectrurn show that transparent crystallized glass containing β-BBO is obtained.The second harmonic generation(SHG) effect of the crystallized glasses is researched by utilizing Maker-fringe technique.It is found that the inversion symmetry of the glass cannot be broken by small grain size and quantity microcystal when calcining at 580~620℃ for 4 h,and the SHG effect can notbeobserved.Theinversion symmetry of the glass was broken by β-BBO in the glass when cal cining at 620℃ for 8 h,which induces the occurrence of SHG effect.The SHG intensity gradually decreases after polishing The results indicate that β-BBO in the glass induced the SHG effect.%采用热处理的方法,制备3TiO_2-45.7BaO-51.3B_2O_3微晶玻璃,利用XRD、透射光谱研究表明,获得了含β-BBO微晶的透明微晶玻璃.采用Maker条纹法研究了微晶玻璃的二阶非线性光学效应,结果表明,当580~620℃热处理4 h时,微晶尺寸小、数量少,不足以破坏玻璃的各向同性,观测不到二次谐波信号;当620℃热处理8 h时,玻璃的β-BBO微晶破坏了玻璃的各向同性,诱导了二次谐波的产生;对该样品进行抛光后,二次谐波信号减弱直至消失,进一步表明玻璃表面的β-BBO微晶是导致玻璃产生二次谐波信号的原因.

  7. A Fluorescence Immunochromatographic Assay Using Europium (III) Chelate Microparticles for Rapid, Quantitative and Sensitive Detection of Creatine Kinase MB.

    Science.gov (United States)

    Lai, Xiao-Hong; Liang, Rong-Liang; Liu, Tian-Cai; Dong, Zhi-Ning; Wu, Ying-Song; Li, Lin-Hai

    2016-05-01

    The isoenzyme creatine kinase MB is very important for diagnosis of acute myocardial infarction (AMI). Some CK-MB immunoassays are sensitive, accurate and available for clinical application, but they are expensive and time-consuming procedures. Furthermore, conventional fluorescence immunochromatographic assays (FL-ICAs) have suffered from background fluorescence interference and low analytical sensitivity. A rapid and simple FL-ICA with Eu (III) chelate polystyrene microparticles was developed to determine CK-MB in 50uL serum samples using a portable test strip reader by measuring the fluorescence peak heights of the test line (HT) and the control line (HC) in 12 min. The assay was reliable with a good correlation coefficient between HT/HC ratio and CK-MB concentration in samples. A linear range was 0.85-100.29 ng/mL for CK-MB, and the LOD was 0.029 ng/mL. The intra- and inter-assay coefficients of variation (CV) were both MB. The system performed well in interference experiments. Furthermore, a highly significant correlation (r = 0.9794, P MB samples. These results indicated that the Eu (III) chelate microparticles-based FL-ICA is simple, fast, highly sensitive, reliable, and reproducible for point-of-care testing of CK-MB concentrations in serum. Graphical Abstract ᅟ.

  8. Progress in Removal Technology of Taste and Odor Compounds-Geosmin and 2-MIB in Drinking Water Source%饮用水源水中致嗅物质去除技术研究进展

    Institute of Scientific and Technical Information of China (English)

    史嘉璐; 龙超; 李爱民

    2012-01-01

    土臭素和2-甲基异莰醇是饮用水中存在的主要致嗅味物质,活性炭吸附是目前研究最多、应用最广泛的嗅味物质去除技术,文章重点介绍了影响活性炭吸附的各种因素,对化学氧化法和生物降解法去除嗅味物质的研究进展进行了简述.%Geosmin and 2-MIB are main compounds causing taste and odor problem in drinking water. Activated carbon has been widely used as an adsorbent to remove odor compounds in drinking water. Effects of various factors on adsorption characteristics of activated carbon for taste and odor compounds were introduced, chemical oxidation and biodegradation technology for removing taste and odor compounds were also reviewed.

  9. Effect of BaO Content on the Properties of a Kind of Microelectronic Packaging Materials%BaO含量对一种微电子封装材料性能的影响

    Institute of Scientific and Technical Information of China (English)

    于东英; 聂永峰; 邓新峰

    2012-01-01

    A kind of microelectronic packaging materials were prepared by traditional solid-state reaction method. The thermal,mechanic and electric properties were measured and the phase and microstructure evolution of the materials were characterized by XRD and SEM. The effect of BaO content on the properties of materials has been studied. The results showed that BaO didn't influence the main phase of quartz so much,but it could promote the formation of BaO·Al2O3·2SiO2 and inhibit the precipitation of the cristobalite phase. Increasing the content of BaO could improve the dielectric properties of materials to a certain extent. However, the glass network would be gradually destroyed, affecting the mechanical and thermal properties and resulting in the bending strength reduction as well as the thermal expansion coefficient decrease.%采用传统固相反应法,制备了一种微电子封装材料,并对其进行了电、力、热性能测试,及XRD、SEM分析表征,具体研究了BaO含量对该材料性能的影响.结果表明:BaO对主晶相石英没有太大的影响,促进钡长石的形成,抑制方石英相的析出,其含量增加能在一定程度上改善材料的介电性能,但会逐渐破坏玻璃网络,影响到材料的力学和热学性能,导致抗弯强度降低,热膨胀系数减小.

  10. The effect of baicalein on the expression of SATB1 in MDA-MB-231 cells

    Institute of Scientific and Technical Information of China (English)

    Xiaoyan Gao; Xingcong Ma; Yinan Ma; Xinghuan Xue; Shuqun Zhang

    2014-01-01

    Objective:Baicalein had been proved to have anti-cancer activity in vitro and in vivo, including the inhibition of malignant proliferation, migration, adhesion and invasion of many kinds of cancer cel s. The special AT-rich sequence binding protein 1 (SATB1) is a tissue-specific expression of nuclear matrix-binding protein and is reported to be a breast cancer“gene group organizer”. Previous studies have shown that SATB1 is involved in the growth, metastasis and prognosis of breast cancer. The present study was aimed to investigate whether baicalein inhibits the proliferation and migration of MDA-MB-231 human breast cancer cel s through down-regulation of the SATB1 expression. Methods:MDA-MB-231 cel s were treated for 24 h, 48 h and 72 h with various concentrations of baicalein (0, 5, 10, 20, 40 and 80μM) respectively. Then, the proliferation and migration of MDA-MB-231 cel s fol owing treatment with baicalein were determined using colorimetric 3-(4, 5-dimethylthia-zol-2-yl) 2, 5-diphenyltetrazolium bromide (MTT) and wound healing assays. Thereafter, western blot analysis was performed to detect the changes of SATB1 protein expression in MDA-MB-231 cel s. Results:Along with the prolongation of time and increase of drug concentration, inhibitory ef ect of baicalein on proliferation and migration of MDA-MB-231 cel s gradual y in-creased, in a time-and dose-dependent manner (P<0.05). Meanwhile, after treated with baicalein in dif erent concentrations for 48 h, the level of SATB1 protein expression of MDA-MB-231 cel s decreased obviously, in a dose-dependent manner (P<0.05). Conclusion:Baicalein inhibits breast cancer cel proliferation and suppresses its invasion and metastasis by reducing cel migration possibly by down-regulation of the SATB1 protein expression, indicating that baicalein is a potential therapeutic agent for human breast cancer.

  11. Methods of creatine kinase-MB analysis to predict mortality in patients with myocardial infarction treated with reperfusion therapy.

    Science.gov (United States)

    Lopes, Renato D; Lokhnygina, Yuliya; Hasselblad, Victor; Newby, Kristin L; Yow, Eric; Granger, Christopher B; Armstrong, Paul W; Hochman, Judith S; Mills, James S; Ruzyllo, Witold; Mahaffey, Kenneth W

    2013-05-02

    Larger infarct size measured by creatine kinase (CK)-MB release is associated with higher mortality and has been used as an important surrogate endpoint in the evaluation of new treatments for ST-segment elevation myocardial infarction (STEMI). Traditional approaches to quantify infarct size include the observed CK-MB peak and calculated CK-MB area under the curve (AUC). We evaluated alternative approaches to quantifying infarct size using CK-MB values, and the relationship between infarct size and clinical outcomes. Of 1,850 STEMI patients treated with reperfusion therapy in the COMplement inhibition in Myocardial infarction treated with Angioplasty (COMMA) (percutaneous coronary intervention (PCI)-treated) and the COMPlement inhibition in myocardial infarction treated with thromboLYtics (COMPLY) (fibrinolytic-treated) trials, 1,718 (92.9%) (COMMA, n = 868; COMPLY, n = 850) had at least five of nine protocol-required CK-MB measures. In addition to traditional methods, curve-fitting techniques were used to determine CK-MB AUC and estimated peak CK-MB. Cox proportional hazards modeling assessed the univariable associations between infarct size and mortality, and the composite of death, heart failure, shock and stroke at 90 days. In COMPLY, CK-MB measures by all methods were significantly associated with higher mortality (hazard ratio range per 1,000 units increase: 1.09 to 1.13; hazard ratio range per 1 standard deviation increase: 1.41 to 1.62; P MB measures were statistically significant in both the COMMA and COMPLY trials. Sophisticated curve modeling is an alternative to infarct-size quantification in STEMI patients, but it provides information similar to that of more traditional methods. Future studies will determine whether the same conclusion applies in circumstances other than STEMI, or to studies with different frequencies and patterns of CK-MB data collection.

  12. Turbo Codes Extended with Outer BCH Code

    DEFF Research Database (Denmark)

    Andersen, Jakob Dahl

    1996-01-01

    The "error floor" observed in several simulations with the turbo codes is verified by calculation of an upper bound to the bit error rate for the ensemble of all interleavers. Also an easy way to calculate the weight enumerator used in this bound is presented. An extended coding scheme is proposed...

  13. Optimization of mask shot count using MB-MDP and lithography simulation

    Science.gov (United States)

    Chua, Gek Soon; Wang, Wei Long; Choi, Byoung IL; Zou, Yi; Tabery, Cyrus; Bork, Ingo; Nguyen, Tam; Fujimura, Aki

    2011-11-01

    In order to maintain manageable process windows, mask shapes at the 20nm technology node and below become so complex that mask write times reach 40 hours or might not be writeable at all since the extrapolated write time reaches 80 hours. The recently introduced Model Based Mask Data Preparation (MB-MDP) technique is able to reduce shot count and therefore mask write time by using overlapping shots. Depending on the amount of shot count reduction the contour of the mask shapes is changed leading to the question how the mask contour influences wafer performance. This paper investigates the tradeoff between mask shot count reduction using MB-MDP and wafer performance using lithography simulation. A typical Source-Mask-Optimization (SMO) result for a 20nm technology will be used as an example.

  14. The 4.8 mb Intraplate Earthquake of 2009 in the Pantanal Wetlands, Western Brazil

    Science.gov (United States)

    Franca, G. S.; Assumpcao, M.; Facincani, E.; Fernandes, C.; Caixeta, D.; von Huelsen, M. G.; Dantas, R. C.

    2009-12-01

    In the Pantanal wetlands a 4.8 mb earthquake occurred on June 16, 2009, with maximum intensity of VI MM followed by only a few aftershocks . The Pantanal basin can be considered a moderately active intraplate seismic zone in Brazil with events larger than 5 occurring every 50 years on average. This recent event was recorded by several short-period and broadband regional stations allowing a good determination of epicenter and focal mechanism. Both the teleseismic (NEIC) and the regional epicenter (Brazilian stations) are consistent with the observed intensities. Clear teleseismic pP phases indicate a focal depth of 5.7 km. The focal mechanism indicates predominantly reverse faulting, similar to the 5.4 mb event of 1964. We will discuss the relationship between the observed regional compressional stresses and the active subsidence of the Pantanal basin.

  15. Work Performance of MB Trac 900 Tractor on Mountains Terrain in Turkey

    Directory of Open Access Journals (Sweden)

    Tolga Ozturk

    2005-01-01

    Full Text Available The forestry works mainly involves such various stages as growing, protection, production, wood harvesting, marketing and utilization of forest products. Wood harvesting is performed by completion of cutting, extraction and transporting stages. The most obvious feature of wood harvesting is that the operations are done under various weather conditions open in the field. The measurements and observations were made on 25 work made under different working conditions during cable pulling with MB Trac 900 forest tractor at the harvesting area. The time determinations were made with continuous time measuring technique. Regression analyses were employed for determining the factors. In this study, the results of studies carried out on the MB Trac 900 forest tractor operated in the Artvin region located in the Northeast Turkey are presented.

  16. The 1. 5-Mb region spanning the myotonic dystrophy locus shows uniform recombination frequency

    Energy Technology Data Exchange (ETDEWEB)

    Shutler, G.G.; MacKenzie, A.E.; Korneluk, R.G. (Univ. of Ottawa (Canada))

    1994-01-01

    The myotonic dystrophy (DM) mutation has been identified as a heritable unstable CTG trinucleotide repeat sequence. The intergenerational amplification of this sequence is an example of a new class of dynamic mutations responsible for human genetic diseases. To ascertain whether recombination activity influences, or is affected by, the presence of this unique sequence, a comprehensive study of the physical and genetic mapping data for the 1.5-Mb region of human chromosome 19q13.3, which contains the DM locus, was conducted. The recombination rate for this region was examined by correlating genetic distance to physical distance for six selected marker loci. The following markers span the DM region: 19qCEN-p[alpha]1.4 (D19S37)-APOC2-CKM-pE0.8 (D19S115)-pGB2.6 (DM)-p134c (D19S51)-19qTER. Initial linear regression analysis of these two parameters failed to reveal a significant linear correlation (coefficient of determination, r[sup 2] = .19), suggesting nonuniform rates of recombination. However, the presence of a recombination hot spot was believed to be restricted to a specific region of the 1.5 Mb studied and had relatively broad confidence intervals, as reflected by low LOD values. A second linear regression analysis using only marker intervals with high LOD scores (Z[sub max] > 22) showed linear correlation (r[sup 2] = .68) for the entire 1.5-Mb region. This analysis indicated a relatively uniform recombination frequency in the 1.5-Mb region spanning the DM locus. Furthermore, the recombination observed were neither under- nor overrepresented on DM chromosomes. Consequently, recombination activity is unlikely to influence, or be affected by, the presence of the DM mutation. 41 refs., 6 figs., 4 tabs.

  17. QCD Corrected $1/m_b$ Contributions to $B\\bbar$--Mixixng

    CERN Document Server

    Kilian, W; Kilian, Wolfgang; Mannel, Thomas

    1993-01-01

    We calculate the QCD corrected effective Hamiltonian for $B\\bbar$--Mixing in heavy quark effective theory including corrections of the order $\\Lambda_{QCD} / m_b$. The matrix elements of the subleading operators are estimated using the vacuum insertion assumption. We show that the major part of the subleading corrections may be absorbed into the heavy meson decay constant $f_B$; the remaining corrections are only due to QCD effects and give an enhancement of $\\Delta M$ of 5\\%.

  18. FEPI-MB: identifying SNPs-disease association using a Markov Blanket-based approach

    Directory of Open Access Journals (Sweden)

    Han Bing

    2011-11-01

    Full Text Available Abstract Background The interactions among genetic factors related to diseases are called epistasis. With the availability of genotyped data from genome-wide association studies, it is now possible to computationally unravel epistasis related to the susceptibility to common complex human diseases such as asthma, diabetes, and hypertension. However, the difficulties of detecting epistatic interaction arose from the large number of genetic factors and the enormous size of possible combinations of genetic factors. Most computational methods to detect epistatic interactions are predictor-based methods and can not find true causal factor elements. Moreover, they are both time-consuming and sample-consuming. Results We propose a new and fast Markov Blanket-based method, FEPI-MB (Fast EPistatic Interactions detection using Markov Blanket, for epistatic interactions detection. The Markov Blanket is a minimal set of variables that can completely shield the target variable from all other variables. Learning of Markov blankets can be used to detect epistatic interactions by a heuristic search for a minimal set of SNPs, which may cause the disease. Experimental results on both simulated data sets and a real data set demonstrate that FEPI-MB significantly outperforms other existing methods and is capable of finding SNPs that have a strong association with common diseases. Conclusions FEPI-MB algorithm outperforms other computational methods for detection of epistatic interactions in terms of both the power and sample-efficiency. Moreover, compared to other Markov Blanket learning methods, FEPI-MB is more time-efficient and achieves a better performance.

  19. Frankincense derived heavy terpene cocktail boosting breast cancer cell(MDA-MB-231) death in vitro简

    Institute of Scientific and Technical Information of China (English)

    Faruck; Lukmanul; Hakkim; Mohammed; Al-Buloshi; Jamal; Al-Sabahi

    2015-01-01

    Objective: To investigate the anti-cancer effect of frankincense derived heavy oil obtained by Soxhlet extraction method on breast cancer cells(MDA-MB-231), and to study its chemical profile using gas chromatography mass spectrometry analysis.Methods: Hexane was used to extract heavy oil from frankincense resin. Chemical profiling of heavy oil was done using Perkin Elmer Clarus GC system with mass spectrometer. MDA-MB-231 cells were treated with different dilutions(1:1 000, 1:1 500,1:1 750, 1:2 000, 1:2 250, 1:2 500, 1:2 750, 1:3 000, 1:3 250) of heavy oil for 24 h. The cells were observed by using light microscopy. Cell viability was measured by MTT assay.Results: Gas chromatography mass spectrometry chemical profiling of frankincense derived heavy oil revealed the presence of terpenes such as a-pinene(61.56%), a-amyrin(20.6%), b-amyrin(8.1%), b-phellandrene(1.47%) and camphene(1.04%). Heavy terpene cocktail induced significant MDA-MB-231 cell death at each concentration tested. Noticeably, very low concentration of Soxhlet derived heavy terpenes elicits considerable cytotoxicity on MDA-MB-231 cells compared to hydro distillated essential oil derived from frankincense resin.Conclusions: Extracting anti-cancer active principle cocktail by simple Soxhlet method is cost effective and less time consuming. Our in vitro anti-cancer data forms the rationale for us to test heavy terpene complex in breast cancer xenograft model in vivo. Furthermore, fractionation and developing frankincense heavy terpene based breast cancer drug is the major goal of our laboratory.

  20. Frankincense derived heavy terpene cocktail boosting breast cancer cell (MDA-MB-231) deathin vitro

    Institute of Scientific and Technical Information of China (English)

    Faruck Lukmanul Hakkim; Mohammed Al-Buloshi; Jamal Al-Sabahi

    2015-01-01

    Objective:To investigate the anti-cancer effect of frankincense derived heavy oil obtained by Soxhlet extraction method on breast cancer cells (MDA-MB-231), and to study its chemical profile using gas chromatography mass spectrometry analysis. Methods: Hexane was used to extract heavy oil from frankincense resin. Chemical profiling of heavy oil was done using Perkin Elmer Clarus GC system with mass spectrometer.MDA-MB-231 cells were treated with different dilutions (1:1 000, 1:1 500, 1:1 750, 1:2 000, 1:2 250, 1:2 500, 1:2 750, 1:3 000, 1:3 250) of heavy oil for 24 h. The cells were observed by using light microscopy. Cell viability was measured byMTT assay. Results: Gas chromatography mass spectrometry chemical profiling of frankincense derived heavy oil revealed the presence of terpenes such asα-pinene (61.56%),α-amyrin (20.6%),β-amyrin (8.1%),β-phellandrene (1.47%) and camphene (1.04%). Heavy terpene cocktail induced significantMDA-MB-231 cell death at each concentration tested. Noticeably, very low concentration of Soxhlet derived heavy terpenes elicits considerable cytotoxicityon MDA-MB-231cells compared to hydro distillated essential oil derived from frankincense resin. Conclusions: Extracting anti-cancer active principle cocktail by simple Soxhlet method is cost effective and less time consuming. Ourin vitro anti-cancer data forms the rationale for us to test heavy terpene complex in breast cancer xenograft modelin vivo. Furthermore, fractionation and developing frankincense heavy terpene based breast cancer drug is the major goal of our laboratory.

  1. Mitochondrial calcium uniporter activity is dispensable for MDA-MB-231 breast carcinoma cell survival.

    Science.gov (United States)

    Hall, Duane D; Wu, Yuejin; Domann, Frederick E; Spitz, Douglas R; Anderson, Mark E

    2014-01-01

    Calcium uptake through the mitochondrial Ca2+ uniporter (MCU) is thought to be essential in regulating cellular signaling events, energy status, and survival. Functional dissection of the uniporter is now possible through the recent identification of the genes encoding for MCU protein complex subunits. Cancer cells exhibit many aspects of mitochondrial dysfunction associated with altered mitochondrial Ca2+ levels including resistance to apoptosis, increased reactive oxygen species production and decreased oxidative metabolism. We used a publically available database to determine that breast cancer patient outcomes negatively correlated with increased MCU Ca2+ conducting pore subunit expression and decreased MICU1 regulatory subunit expression. We hypothesized breast cancer cells may therefore be sensitive to MCU channel manipulation. We used the widely studied MDA-MB-231 breast cancer cell line to investigate whether disruption or increased activation of mitochondrial Ca2+ uptake with specific siRNAs and adenoviral overexpression constructs would sensitize these cells to therapy-related stress. MDA-MB-231 cells were found to contain functional MCU channels that readily respond to cellular stimulation and elicit robust AMPK phosphorylation responses to nutrient withdrawal. Surprisingly, knockdown of MCU or MICU1 did not affect reactive oxygen species production or cause significant effects on clonogenic cell survival of MDA-MB-231 cells exposed to irradiation, chemotherapeutic agents, or nutrient deprivation. Overexpression of wild type or a dominant negative mutant MCU did not affect basal cloning efficiency or ceramide-induced cell killing. In contrast, non-cancerous breast epithelial HMEC cells showed reduced survival after MCU or MICU1 knockdown. These results support the conclusion that MDA-MB-231 breast cancer cells do not rely on MCU or MICU1 activity for survival in contrast to previous findings in cells derived from cervical, colon, and prostate cancers and

  2. Mitochondrial calcium uniporter activity is dispensable for MDA-MB-231 breast carcinoma cell survival.

    Directory of Open Access Journals (Sweden)

    Duane D Hall

    Full Text Available Calcium uptake through the mitochondrial Ca2+ uniporter (MCU is thought to be essential in regulating cellular signaling events, energy status, and survival. Functional dissection of the uniporter is now possible through the recent identification of the genes encoding for MCU protein complex subunits. Cancer cells exhibit many aspects of mitochondrial dysfunction associated with altered mitochondrial Ca2+ levels including resistance to apoptosis, increased reactive oxygen species production and decreased oxidative metabolism. We used a publically available database to determine that breast cancer patient outcomes negatively correlated with increased MCU Ca2+ conducting pore subunit expression and decreased MICU1 regulatory subunit expression. We hypothesized breast cancer cells may therefore be sensitive to MCU channel manipulation. We used the widely studied MDA-MB-231 breast cancer cell line to investigate whether disruption or increased activation of mitochondrial Ca2+ uptake with specific siRNAs and adenoviral overexpression constructs would sensitize these cells to therapy-related stress. MDA-MB-231 cells were found to contain functional MCU channels that readily respond to cellular stimulation and elicit robust AMPK phosphorylation responses to nutrient withdrawal. Surprisingly, knockdown of MCU or MICU1 did not affect reactive oxygen species production or cause significant effects on clonogenic cell survival of MDA-MB-231 cells exposed to irradiation, chemotherapeutic agents, or nutrient deprivation. Overexpression of wild type or a dominant negative mutant MCU did not affect basal cloning efficiency or ceramide-induced cell killing. In contrast, non-cancerous breast epithelial HMEC cells showed reduced survival after MCU or MICU1 knockdown. These results support the conclusion that MDA-MB-231 breast cancer cells do not rely on MCU or MICU1 activity for survival in contrast to previous findings in cells derived from cervical, colon, and

  3. The 1.5-Mb region spanning the myotonic dystrophy locus shows uniform recombination frequency.

    Science.gov (United States)

    Shutler, G G; MacKenzie, A E; Korneluk, R G

    1994-01-01

    The myotonic dystrophy (DM) mutation has been identified as a heritable unstable CTG trinucleotide repeat sequence. The intergenerational amplification of this sequence is an example of a new class of dynamic mutations responsible for human genetic diseases. To ascertain whether recombination activity influences, or is affected by, the presence of this unique sequence, a comprehensive study of the physical and genetic mapping data for the 1.5-Mb region of human chromosome 19q13.3, which contains the DM locus, was conducted. The recombination rate for this region was examined by correlating genetic distance to physical distance for six selected marker loci. The following markers span the DM region: 19qCEN-p alpha 1.4 (D19S37)-APOC2-CKM-pE0.8 (D19S115)-pGB2.6 (DM)-p134c (D19S51)-19qTER. Initial linear regression analysis of these two parameters failed to reveal a significant linear correlation (coefficient of determination, r2 = .19), suggesting nonuniform rates of recombination. However, the presence of a recombination hot spot was believed to be unlikely, as the marker-to-marker pairs that showed the greatest deviation in recombination frequency were not restricted to a specific region of the 1.5 Mb studied and had relatively broad confidence intervals, as reflected by low LOD values. A second linear regression analysis using only marker intervals with high LOD scores (Zmax > 22) showed linear correlation (r2 = .68) for the entire 1.5-Mb region. This analysis indicated a relatively uniform recombination frequency in the 1.5-Mb region spanning the DM locus. Furthermore, the recombinations observed were neither under- nor overrepresented on DM chromosomes. Consequently, recombination activity is unlikely to influence, or be affected by, the presence of the DM mutation.

  4. Primordial density and BAO reconstruction

    CERN Document Server

    Zhu, Hong-Ming; Chen, Xuelei

    2016-01-01

    We present a new method to reconstruct the primordial (linear) density field using the estimated nonlinear displacement field. The divergence of the displacement field gives the reconstructed density field. We solve the nonlinear displacement field in the 1D cosmology and show the reconstruction results. The new reconstruction algorithm recovers a lot of linear modes and reduces the nonlinear damping scale significantly. The successful 1D reconstruction results imply the new algorithm should also be a promising technique in the 3D case.

  5. Characterization of immunoglobulin G antibodies to Plasmodium falciparum sporozoite surface antigen MB2 in malaria exposed individuals

    Directory of Open Access Journals (Sweden)

    John Chandy C

    2009-10-01

    Full Text Available Abstract Background MB2 protein is a sporozoite surface antigen on the human malaria parasite Plasmodium falciparum. MB2 was identified by screening a P. falciparum sporozoite cDNA expression library using immune sera from a protected donor immunized via the bites of P. falciparum-infected irradiated mosquitoes. It is not known whether natural exposure to P. falciparum also induces the anti-MB2 response and if this response differs from that in protected individuals immunized via the bites of P. falciparum infected irradiated mosquitoes. The anti-MB2 antibody response may be part of a robust protective response against the sporozoite. Methods Fragments of polypeptide regions of MB2 were constructed as recombinant fusions sandwiched between glutathione S-transferase and a hexa histidine tag for bacterial expression. The hexa histidine tag affinity purified proteins were used to immunize rabbits and the polyclonal sera evaluated in an in vitro inhibition of sporozoite invasion assay. The proteins were also used in immunoblots with sera from a limited number of donors immunized via the bites of P. falciparum infected irradiated mosquitoes and plasma and serum obtained from naturally exposed individuals in Kenya. Results Rabbit polyclonal antibodies targeting the non-repeat region of the basic domain of MB2 inhibited sporozoites entry into HepG2-A16 cells in vitro. Analysis of serum from five human volunteers that were immunized via the bites of P. falciparum infected irradiated mosquitoes that developed immunity and were completely protected against subsequent challenge with non-irradiated parasite also had detectable levels of antibody against MB2 basic domain. In contrast, in three volunteers not protected, anti-MB2 antibodies were below the level of detection. Sera from protected volunteers preferentially recognized a non-repeat region of the basic domain of MB2, whereas plasma from naturally-infected individuals also had antibodies that

  6. [Effects of mammalian-target-of-rapamycin pathway on lapatinib resistance in breast cancer MDA-MB-231 cells].

    Science.gov (United States)

    Wang, Jian-dong; Wang, Quan-sheng; Bai, Yi-zhou; Kou, De-qiang; Li, Xi-ru; Chen, Lin; Li, Rong

    2013-06-25

    To establish a lapatinib resistance cell line for elucidating the mechanisms of drug resistance of lapatinib in human breast cancer cells. The human breast cancer MDA-MB-231 cells were exposed in an incremental dose of lapatinib to establish a lapatinib resistance rMDA-MB-231 cell line. The assay of methyl thiazolyl tetrazolium (MTT) was used to detect the cytotoxic activity of lapatinib against MDA-MB-231 and rMDA-MB-231 cells. The protein expression was detected by Western blot. Small interfering RNA was used to specifically knock down mammalian-target-of-rapamycin (mTOR) in rMDA-MB-231 cells. Apoptosis was determined by fluorescein isothiocyanate (FITC)-annexin V/PI staining and flow cytometry. The human breast cancer lapatinib resistance cell line rMDA-MB-231 was induced by lapatinib. The half maximal inhibitory concentration (IC50) values of lapatinib against MDA-MB-231 and rMDA-MB-231 cells were (6.1 ± 0.6) and (34.9 ± 2.7) µmol/L respectively (P MB-231 cells, the protein expression of mTOR in rMDA-MB-231 cells was significantly up-regulated. The protein expression of mTOR was significantly down-regulated by specific siRNA duplexes in rMDA-MB-231 cells. After siRNA interference, 20 µmol/L lapatinib was added into control, negative siRNA control and mTOR-targeted siRNA groups respectively. The percents of cell apoptosis in control, negative control and targeted siRNA groups were 13.4% ± 2.5%, 14.2% ± 2.8% and 34.6% ± 5.8% respectively, there was no significance between the first two groups (P > 0.05) , and there was significant difference between the control and targeted siRNA group (P MB-231 cells. And the down-regulation of mTOR increases the apoptotic death of lapatinib against rMDA-MB-231 cells.

  7. Model-based mask data preparation (MB-MDP) and its impact on resist heating

    Science.gov (United States)

    Fujimura, Aki; Kamikubo, Takashi; Bork, Ingo

    2011-04-01

    Complex mask shapes will be required on critical layer masks for 20nm logic node, threatening to explode the mask write times. Model-Based Mask Data Preparation (MB-MDP) has been introduced to reduce the shot count required to write complex masks while simultaneously improving resolution and dose margin of sub-100nm features. For production use of MB-MDP, a number of questions have been raised and answered. This paper summarizes these potential issues and their resolutions. In particular, the paper takes an in-depth look at one of the questions: impact of overlapping shots on heating effect. The paper concludes that while heating effect is an important issue for all e-beam writing even with conventional non-overlapping shots, overall dose density per unit time over microns of space is the principal driver behind heating effects. Highly local shot density and shot sequencing does not affect heating significantly, particularly for smaller shots. MB-MDP does not introduce any additional concerns.

  8. Neem Seed Oil Induces Apoptosis in MCF-7 and MDA MB-231 Human Breast Cancer Cells

    Science.gov (United States)

    Sharma, Ramesh; Kaushik, Shweta; Shyam, Hari; Agarwal, Satish; Balapure, Anil Kumar

    2017-08-27

    Background: In traditional Indian medicine, azadirachta indica (neem) is known for its wide range of medicinal properties. Various parts of neem tree including its fruit, seed, bark, leaves, and root have been shown to possess antiseptic, antiviral, antipyretic, anti-inflammatory, antiulcer, antimalarial, antifungal and anticancer activity. Materials and Methods: MCF-7 and MDA MB-231 cells were exposed to various concentrations of 2% ethanolic solution of NSO (1-30 μl/ml) and further processed for cell viability, cell cycle and apoptosis analysis. In addition, cells were analyzed for alteration in Mitochondrial Membrane Potential (MMP) and generation of Reactive Oxygen Species (ROS) using JC-1 and DCFDA staining respectively. Results: NSO give 50% inhibition at 10 μl/ml and 20 μl/ml concentration in MCF-7 and MDA MB-231 cells respectively and, arrests cells at G0/G1 phase in both the cell types. There was a significant alteration in mitochondrial membrane potential that leads to the generation of ROS and induction of apoptosis in NSO treated MCF-7 and MDA MB-231 cells. Conclusion: The results showed that NSO inhibits the growth of human breast cancer cells via induction of apoptosis and G1 phase arrest. Collectively these results suggest that NSO could potentially be used in the management of breast cancer. Creative Commons Attribution License

  9. Cytotoxicity of Biologically Synthesized Silver Nanoparticles in MDA-MB-231 Human Breast Cancer Cells

    Directory of Open Access Journals (Sweden)

    Sangiliyandi Gurunathan

    2013-01-01

    Full Text Available Silver nanoparticles (AgNPs have been used as an antimicrobial and disinfectant agents. However, there is limited information about antitumor potential. Therefore, this study focused on determining cytotoxic effects of AgNPs on MDA-MB-231 breast cancer cells and its mechanism of cell death. Herein, we developed a green method for synthesis of AgNPs using culture supernatant of Bacillus funiculus, and synthesized AgNPs were characterized by various analytical techniques such as UV-visible spectrophotometer, particle size analyzer, and transmission electron microscopy (TEM. The toxicity was evaluated using cell viability, metabolic activity, and oxidative stress. MDA-MB-231 breast cancer cells were treated with various concentrations of AgNPs (5 to 25 μg/mL for 24 h. We found that AgNPs inhibited the growth in a dose-dependent manner using MTT assay. AgNPs showed dose-dependent cytotoxicity against MDA-MB-231 cells through activation of the lactate dehydrogenase (LDH, caspase-3, reactive oxygen species (ROS generation, eventually leading to induction of apoptosis which was further confirmed through resulting nuclear fragmentation. The present results showed that AgNPs might be a potential alternative agent for human breast cancer therapy.

  10. Detection of genomic variation by selection of a 9 mb DNA region and high throughput sequencing.

    Directory of Open Access Journals (Sweden)

    Sergey I Nikolaev

    Full Text Available Detection of the rare polymorphisms and causative mutations of genetic diseases in a targeted genomic area has become a major goal in order to understand genomic and phenotypic variability. We have interrogated repeat-masked regions of 8.9 Mb on human chromosomes 21 (7.8 Mb and 7 (1.1 Mb from an individual from the International HapMap Project (NA12872. We have optimized a method of genomic selection for high throughput sequencing. Microarray-based selection and sequencing resulted in 260-fold enrichment, with 41% of reads mapping to the target region. 83% of SNPs in the targeted region had at least 4-fold sequence coverage and 54% at least 15-fold. When assaying HapMap SNPs in NA12872, our sequence genotypes are 91.3% concordant in regions with coverage > or = 4-fold, and 97.9% concordant in regions with coverage > or = 15-fold. About 81% of the SNPs recovered with both thresholds are listed in dbSNP. We observed that regions with low sequence coverage occur in close proximity to low-complexity DNA. Validation experiments using Sanger sequencing were performed for 46 SNPs with 15-20 fold coverage, with a confirmation rate of 96%, suggesting that DNA selection provides an accurate and cost-effective method for identifying rare genomic variants.

  11. 基于MB-LDA模型的微博主题挖掘%Topic Mining for Microblog Based on MB-LDA Model

    Institute of Scientific and Technical Information of China (English)

    张晨逸; 孙建伶; 丁轶群

    2011-01-01

    As microblog grows more popular, services like Twitter have become information providers on a web scale. Early work on microblog focused more on its user relationship and community structure, without considering the value of content. So the research on microblog requires a change from solely user's relationship analysis to its content mining. Although traditional text mining methods have been studied well, no algorithm is designed specially for microblog data, which contain structured information on social network besides plain text. In this paper, we propose a novelprobabilistic generative model based on LDA, called MB-LDA, which is suitable to model the microblog data and takes both contact relation and document relation into consideration to help topic mining in microblog. We present a Gibbs sampling implementation for inference of our model, and find not only the topics of microblog, but also the topics focused by contactors according to the final results. Besides, our model can be extended to many texts associated with social networking such as E-mails and forum posts. Experimental results on actual dataset show that MB-LDA model can offer an effective solution to topic mining for microblog.%随着微博的日趋流行,Twitter等微博网站已成为海量信息的发布体,对微博的研究也需要从单一的用户关系分析向微博本身内容的挖掘进行转变.在数据挖掘领域,尽管传统文本的主题挖掘已经得到了广泛的研究,但对于微博这种特殊的文本,因其本身带有一些结构化的社会网络方面的信息,传统的文本挖掘算法不能很好地对它进行建模.提出了一个基于LDA的微博生成模型MB-LDA,综合考虑了微博的联系人关联关系和文本关联关系,来辅助进行微博的主题挖掘.采用吉布斯抽样法对模型进行推导,不仅能挖掘出微博的主题,还能挖掘出联系人关注的主题.此外,模型还能推广到许多带有社交网络性质的文本中.在

  12. A 54 Mb 11qter duplication and 0.9 Mb 1q44 deletion in a child with laryngomalacia and agenesis of corpus callosum

    Directory of Open Access Journals (Sweden)

    Lall Meena

    2011-09-01

    Full Text Available Abstract Background Partial Trisomy 11q syndrome (or Duplication 11q has defined clinical features and is documented as a rare syndrome by National Organization of Rare Disorders (NORD. Deletion 1q44 (or Monosomy 1q44 is a well-defined syndrome, but there is controversy about the genes lying in 1q44 region, responsible for agenesis of the corpus callosum. We report a female child with the rare Partial Trisomy 11q syndrome and Deletion 1q44 syndrome. The genomic imbalance in the proband was used for molecular characterization of the critical genes in 1q44 region for agenesis of corpus callosum. Some genes in 11q14q25 may be responsible for laryngomalacia. Results We report a female child with dysmorphic features, microcephaly, growth retardation, seizures, acyanotic heart disease, and hand and foot deformities. She had agenesis of corpus callosum, laryngomalacia, anterior ectopic anus, esophageal reflux and respiratory distress. Chromosome analysis revealed a derivative chromosome 1. Her karyotype was 46,XX,der(1t(1;11(q44;q14pat. The mother had a normal karyotype and the karyotype of the father was 46,XY,t(1;11(q44;q14. SNP array analysis showed that the proband had a 54 Mb duplication of 11q14q25 and a 0.9 Mb deletion of the submicroscopic subtelomeric 1q44 region. Fluorescence Insitu Hybridisation confirmed the duplication of 11qter and deletion of 1qter. Conclusion Laryngomalacia or obstruction of the upper airway is the outcome of increased dosage of some genes due to Partial Trisomy 11q Syndrome. In association with other phenotypic features, agenesis of corpus callosum appears to be a landmark phenotype for Deletion 1q44 syndrome, the critical genes lying proximal to SMYD3 in 1q44 region.

  13. Influence of MB-value of Manufactured Sand on the Shrinkage and Cracking of High Strength Concrete

    Institute of Scientific and Technical Information of China (English)

    WANG Jiliang; YANG Zhifeng; NIU Kaimin; KE Guoju; ZHOU Mingkai

    2009-01-01

    The relation between methylene blue (MB) value of MS and its limestone dust content and clay content was investigated. The effects of MB value ranging from 0.35 to 2.5 on the workability of fresh concrete and crack propagation characteristics at the age of 24 hours, and effects on the mechanical properties, dry shrinkage of the harden concrete were tested. The experimental results show that the MB value is not related with the limestone dust content of MS, but in direct proportion to clay content. With the increase of MB value, the concrete workability decreases, and the flexural strength and 7 d compressive strength reduce markedly, whearas the 28 d compressive strength is not affected. When the MB-value is less than or equal to 1.35, the change of the MB-valuehas a little influence on early plastic cracking and dry shrinkage property of concrete, but when the MB-value is more than 1.35, the tendency of plastic cracking and dry shrinkage is remarkable.

  14. Evaluation of the benefit of the bispyridinium compound MB327 for the antidotal treatment of nerve agent-poisoned mice.

    Science.gov (United States)

    Kassa, Jiri; Pohanka, Miroslav; Timperley, Christopher M; Bird, Mike; Green, A Christopher; Tattersall, John E H

    2016-06-01

    The potency of the bispyridinium non-oxime compound MB327 [1,1'-(propane-1,3-diyl)bis(4-tert-butylpyridinium) diiodide] to increase the therapeutic efficacy of the standard antidotal treatment (atropine in combination with an oxime) of acute poisoning with organophosphorus nerve agents was studied in vivo. The therapeutic efficacy of atropine alone - or atropine in combination with an oxime, MB327, or both an oxime and MB237 - was evaluated by the determination of LD50 values of several nerve agents (tabun, sarin and soman) in mice with and without treatment. The addition of MB327 increased the therapeutic efficacy of atropine alone, and atropine in combination with an oxime, against all three nerve agents, although differences in the LD50 values only reached statistical significance for sarin. In conclusion, the addition of the compound MB327 to the standard antidotal treatment of acute poisonings with nerve agents was beneficial regardless of the chemical structure of the nerve agent, although at the dose employed, MB327 in combination with atropine, or atropine and an oxime, provided only a modest increase in protection ratio. These results from mice, and previous ones from guinea-pigs, provide consistent evidence for additional, albeit modest, efficacy resulting from the inclusion of the antinicotinic compound MB327 in standard antidotal therapy. Given the typically steep probit slope for the dose-lethality relationship for nerve agents, such modest increases in protection ratio could provide significant survival benefit.

  15. The applications and clinical significance of IMA, H-FABP joint CK-MB in acute myocardial infarction

    Institute of Scientific and Technical Information of China (English)

    Xiao-Hui Zeng; Yi Zhang

    2016-01-01

    ABSTRACT Objective:To investigate the applications and clinical significance of ischemia-modified protein (IMA), heart-type fatty acid binding protein (H-FABP) joint creatine kinase isoenzyme (CK-MB) in acute myocardial infarction (AMI).Methods:From January 2014 to December 2014, 84 clinical materials of patients with AMI in Department of cardiology were collected, and 80 cases of normal projects were the control group. IMA was determinated with albumin-cobalt ion binding assay. The levels of H-FABP and CK-MB of 84 cases of AMI patients and 80 cases of normal subjects were determinated with immunoturbidimetric. The value of IMA, H-FABP and CK-MB of AMI patients were analyzed with subject-specific curve (ROC). Results:The levels of serum IMA, H-FABP and CK-MB of AMI group were significantly higher than those of the control group. Differences showed statistical significance (P < 0.05). The levels of serum IMA, H-FABP and CK-MB increased as the increase of coronary lesion vessels in patients with AMI (P< 0.05). The levels of IMA, H-FABP and CK-MB of the death group were significantly higher than those of the surviving group (P < 0.05). ROC curve analysis showed that when IMA, H-FABP and CK-MB joint detections of sensitivity, specificity, positive predictive value and negative predictive value were greater than single-index detection (P< 0.05).Conclusions:The expression levels of IMA, H-FABP and CK-MB are closely related to the occurrence and development of disease progression in patients with AMI. IMA, H-FABP joint CK-MB detection can improve the sensitivity and specificity for early diagnosis of AMI.

  16. Analysis on the finalized date and first carving time of Wei sheng bao jian (The Precious Mirror of ;Hygiene)%《卫生宝鉴》成书及初刊年代辨析

    Institute of Scientific and Technical Information of China (English)

    温雯婷; 虞舜

    2015-01-01

    The Wei sheng bao jian ( The Precious Mirror of Hygiene) , written by Luo Tianyi should be finished in the spring of 1283. The wrong date of 1281 was a mistake coming from Yanjian’ s Preface, while the complete date of 1343 was a misunderstanding on “Guiwei year of the Zhiyuan reign” in Wan-gyun’ s Preface. The hypothesis that Wei sheng bao jian was first carved in 1294 or before the Tang ye ben cao ( Materia Medica for Decoctions) is also groundless. The book was probably completed, carved and dis-tributed in the same year, or no later than 1307, even if it was not carved in the same year right after its completion.%罗天益所撰《卫生宝鉴》应成书于1283年春。成书于1281年之说,是据《砚坚序》的错误推理;成书于1343年之说,是对《王恽序》“至元癸未”的误解。《卫生宝鉴》初刊于《汤液本草》之前或1294年之说,均不足为据。此书成书当年可能即刊刻行世;即便成书当年没有刊刻,初刊年也不晚于1307年。

  17. Impact du BAO électronique sur l’intention d’achat du consommateur:Le rôle modérateur de l’âge et du genre

    Directory of Open Access Journals (Sweden)

    Manel Hamouda

    2014-03-01

    Full Text Available Les variables sociodémographiques tels que l’âge et le genre sont considérées comme des variables très importantes en comportement du consommateur que ce soit dans un contexte réel ou dans un environnement virtuel. A cet égard, cette recherche se propose d’étudier le rôle de l’âge et du genre dans la relation entre l’intention d’achat du consommateur et le Bouche à oreille électronique (BAO électronique. Les données utilisées dans cette recherche ont été collectées à travers une étude empirique menée sur un échantillon de 204 internautes. Les résultats indiquent que le genre et l’âge constituent deux variables susceptibles de modérer l’intention d’achat du consommateur suite à une exposition à un BAO électronique. Les implications inhérentes pour les chercheurs ainsi que pour les praticiens ont été par ailleurs suggérées.

  18. Disparate SAR Data of Griseofulvin Analogues for the Dermatophytes Trichophyton mentagrophytes, T. rubrum, and MDA-MB-231 Cancer Cells

    DEFF Research Database (Denmark)

    Rønnest, Mads Holger; Raab, Marc S.; Anderhub, Simon

    2012-01-01

    Griseofulvin and 53 analogues of this compound have been tested against the pathogenic dermatophytes Trichophyton rubrum and Trichophyton mentagrophytes as well as against the breast cancer cell line MDA-MB-231. The modifications to griseofulvin include the 4, 5, 6, 2', 3', and 4' positions...... of analogues showed increased activity against the cancer cell line MDA-MB-231, highlighted by 2'-benzyloxy-2'-demethoxy-griseofulvin, which showed low activity against both fungi but was among the most potent compounds against MDA-MB-231 cancer cells. Tubulin has been proposed as the target of griseofulvin...

  19. Clinician-scientist MB/PhD training in the UK: a nationwide survey of medical school policy.

    Science.gov (United States)

    Barnett-Vanes, Ashton; Ho, Guiyi; Cox, Timothy M

    2015-12-30

    This study surveyed all UK medical schools regarding their Bachelor of Medicine (MB), Doctor of Philosophy (PhD) (MB/PhD) training policy in order to map the current training landscape and to provide evidence for further research and policy development. Deans of all UK medical schools registered with the Medical Schools Council were invited to participate in this survey electronically. The number of medical schools that operate institutional MB/PhD programmes or permit self-directed student PhD intercalation. Medical school recruitment procedures and attitudes to policy guidance. 27 of 33 (81%) registered UK medical schools responded. Four (14%) offer an institutional MB/PhD programme. However, of those without institutional programmes, 17 (73%) permit study interruption and PhD intercalation: two do not (one of whom had discontinued their programme in 2013), three were unsure and one failed to answer the question. Regarding student eligibility, respondents cited high academic achievement in medical studies and a bachelor's or master's degree. Of the Medical schools without institutional MB/PhD programmes, 5 (21%) have intentions to establish a programme, 8 (34%) do not and 3 were unsure, seven did not answer. 19 medical schools (70%) considered national guidelines are needed for future MB/PhD programme development. We report the first national survey of MB/PhD training in the UK. Four medical schools have operational institutional MB/PhD programmes, with a further five intending to establish one. Most medical schools permit study interruption and PhD intercalation. The total number MB/PhD students yet to graduate from medical school could exceed 150, with 30 graduating per year. A majority of medical school respondents to this survey believe national guidelines are required for MB/PhD programme development and implementation. Further research should focus on the MB/PhD student experience. Discussion regarding local and national MB/PhD policies between medical

  20. The MB2 gene family of Plasmodium species has a unique combination of S1 and GTP-binding domains

    Directory of Open Access Journals (Sweden)

    Ogunjumo Oluwasanmi

    2004-06-01

    Full Text Available Abstract Background Identification and characterization of novel Plasmodium gene families is necessary for developing new anti-malarial therapeutics. The products of the Plasmodium falciparum gene, MB2, were shown previously to have a stage-specific pattern of subcellular localization and proteolytic processing. Results Genes homologous to MB2 were identified in five additional parasite species, P. knowlesi, P. gallinaceum, P. berghei, P. yoelii, and P. chabaudi. Sequence comparisons among the MB2 gene products reveal amino acid conservation of structural features, including putative S1 and GTP-binding domains, and putative signal peptides and nuclear localization signals. Conclusions The combination of domains is unique to this gene family and indicates that MB2 genes comprise a novel family and therefore may be a good target for drug development.

  1. Determination of buckling in the IPEN/MB-01 Reactor in cylindrical configuration

    Energy Technology Data Exchange (ETDEWEB)

    Purgato, Rafael Turrini; Bitelli, Ulysses d' Utra; Aredes, Vitor Ottoni; Silva, Alexandre F. Povoa da; Santos, Diogo Feliciano dos; Mura, Luis Felipe L.; Jerez, Rogerio, E-mail: rtpurgato@ipen.br, E-mail: ubitelli@ipen.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2013-07-01

    One of the key parameters in reactor physics is the buckling of a reactor core. It is related to important parameters such as reaction rates, nuclear power operation, fuel burning, among others. In a critical reactor, the buckling depends on the geometric and material characteristics of the reactor core. This paper presents the results of experimental buckling in the reactor IPEN/MB-01 nuclear reactor in its cylindrical configuration with 28 fuel rods along its diameter. The IPEN/MB-01 is a zero power reactor designed to operate at a maximum power of 100 watts, it is a versatile nuclear facility which allows the simulation of all the characteristics of a large nuclear power reactor and ideal for this type of measurement. We conducted a mapping of neutron flux inside the reactor and thereby determined the total buckling of the cylindrical configuration. The reactor was operated for an hour. Then, the activation of the fuel rods was measured by gamma spectrometry on a rod scanner HPGe detector. We analyzed the gamma photons of the {sup 239}Np (276,6 keV) for neutron capture and the {sup 143}Ce (293,3 keV) for fission on both {sup 238}U and {sup 235}U, respectively. We analyzed the axial and radial directions. Other measurements were performed using wires and gold foils in the radial and axial directions of the reactor core. The results showed that the cylindrical configuration compared to standard rectangular configuration of the IPEN/MB-01 reactor has a higher neutron economy, since in this configuration there is less leakage of neutrons. The Buckling Total obtained from the three methods was 95.84 ± 2.67 m{sup -2}. (author)

  2. Glutamine deprivation sensitizes human breast cancer MDA-MB-231 cells to TRIAL-mediated apoptosis.

    Science.gov (United States)

    Dilshara, Matharage Gayani; Jeong, Jin-Woo; Prasad Tharanga Jayasooriya, Rajapaksha Gedara; Neelaka Molagoda, Ilandarage Menu; Lee, Seungheon; Park, Sang Rul; Choi, Yung Hyun; Kim, Gi-Young

    2017-02-13

    Tumor cell metabolism is a promising target for various cancer treatments. Apart from aerobic glycolysis, cancer cell growth is dependent on glutamine (Gln) supply, leading to their survival and differentiation. Therefore, we examined whether treatment with TNF-related apoptosis-inducing ligand (TRAIL) sensitizes MDA-MB-231 cells to apoptosis under Gln deprivation condition (TRAIL/Gln deprivation). Gln deprivation decreased cell proliferation as expected, but did not induce remarkable cell death. TRAIL/Gln deprivation, however, significantly increased growth inhibition and morphological shrinkage of MDA-MB-231 cells compared to those induced by treatment with either Gln deprivation or TRAIL alone. Moreover, TRAIL/Gln deprivation upregulated the apoptotic sub-G1 phase accompanied with a remarkable decrease of pro-caspase-3, pro-caspase-9, and anti-apoptotic xIAP, and Bcl-2. Increased cleavage of PARP and pro-apoptotic Bid protein expression suggests that TRAIL/Gln deprivation triggers mitochondrion-mediated apoptosis in MDA-MB-231 cells. Additionally, TRAIL/Gln deprivation upregulated the expression of endoplasmic reticulum (ER) stress markers such as ATF4 and phosphorylated eIF2α, thereby enhancing the C/EBP homologous protein (CHOP) protein level. Transient knockdown of CHOP partically reversed TRAIL/Gln deprivation-mediated apoptosis. Accordingly, TRAIL/Gln deprivation enhanced the expression of death receptor 5 (DR5) and transient knockdown of DR5 completely restored TRAIL/Gln deprivation-mediated apoptosis. Taken together, our results suggest that Gln deprivation conditions can be used for the development of new therapies for TRAIL-resistant cancers.

  3. Mitochondrial delivery of doxorubicin via triphenylphosphine modification for overcoming drug resistance in MDA-MB-435/DOX cells.

    Science.gov (United States)

    Han, Min; Vakili, Mohammad Reza; Soleymani Abyaneh, Hoda; Molavi, Ommoleila; Lai, Raymond; Lavasanifar, Afsaneh

    2014-08-04

    In this study, doxorubicin (DOX) was conjugated to a lipophilic triphenylphosphonium (TPP) that is selectively taken up by the mitochondrial membrane of cells. This new derivative of DOX, i.e., TPP-DOX, was characterized by infrared spectroscopy (IR), nuclear magnetic resonance ((1)H NMR, (13)C NMR), and mass spectrometry. The effect of TPP modification on DOX cell uptake, intracellular trafficking, eventual DOX induced cytotoxicity, and the level of cleaved caspase 3 and PARP in wild type MDA-MB-435/WT and DOX resistant MDA-MB-435/DOX cells was then evaluated and compared to that for free DOX. In general, free DOX cellular uptake appeared to be significantly higher in MDA-MB-435/WT than MDA-MB-435/DOX cells. Moreover, free DOX was able to enter the nucleus of MDA-MB-435/WT cells, but in MDA-MB-435/DOX cells, it was confined within the cytoplasm. The TPP-DOX, on the other hand, was localized in the cytoplasm of both cell phenotypes and showed preferential distribution to the mitochondria. Correspondingly, in MDA-MB-435/DOX cells, an enhanced cytotoxicity was observed for TPP-DOX (IC50 of 33.6 and 21.0 μM at 48 and 72 h incubation, respectively) in comparison to free DOX (IC50 of 126.7 and 77.96 μM at 48 and 72 h incubation, respectively). This observation was accompanied by the increased level of cleaved caspase 3 and PARP indicating enhanced apoptosis in both cell lines, particularly that of MDA-MB-435/DOX, for TPP-DOX compared to free DOX following 24 h treatment. The present study highlights promising application of TPP-DOX in reversing drug resistance in tumor cells.

  4. Study on the isothermal forging process of MB26 magnesium alloy adaptor

    Directory of Open Access Journals (Sweden)

    Xu Wenchen

    2015-01-01

    Full Text Available The isothermal forging process is an effective method to manufacture complex-shaped components of hard-to-work materials, such as magnesium alloys. This study investigates the isothermal forging process of an MB26 magnesium alloy adaptor with three branches. The results show that two-step forging process is appropriate to form the adaptor forging, which not only improves the filling quality but also reduces the forging load compared with one-step forging process. Moreover, the flow line is distributed along the contour of the complex-shaped adaptor forging.

  5. Application of Fisher method to discriminating earthquakes and explosions using criterion mb/Ms

    Institute of Scientific and Technical Information of China (English)

    BIAN Yin-ju

    2005-01-01

    We try to give a quantitative and global discrimination function by studying mb/Ms data using Fisher method that is a kind of pattern recognition methods. The reliability of the function is also analyzed. The results show that this criterion works well and has a global feature, which can be used as first-level filtering criterions in event identification. The quantitative and linear discrimination function makes it possible to identify events automatically and achieve the goal to react the events quickly and effectively.

  6. The two conformers of acetanilide unraveled using LA-MB-FTMW spectroscopy

    Science.gov (United States)

    Cabezas, C.; Varela, M.; Caminati, W.; Mata, S.; López, J. C.; Alonso, J. L.

    2011-07-01

    Acetanilide has been investigated by laser ablation molecular beam Fourier transform microwave LA-MB-FTMW spectroscopy. The rotational spectrum of both trans and cis conformers have been analyzed to determine the rotational and 14N quadrupole coupling the constants. The spectrum of the less abundant cis conformer has been assigned for the first time. The doublets observed for this conformer have been interpreted in terms of the tunneling motion between two equivalent non-planar conformations through a small barrier in which the acetamide group and phenyl ring plane are perpendicular. The results are compared with those of the related formanilide.

  7. Design and Validation of an Optimized MB-OFDM Ultra Wideband Transceiver System

    OpenAIRE

    Liang, Guixuan

    2013-01-01

    Esta tesis está incluida dentro del campo del campo de Multiband Orthogonal Frequency Division Multiplexing Ultra Wideband (MB-OFDM UWB), el cual ha adquirido una gran importancia en las comunicaciones inalámbricas de alta tasa de datos en la última década. UWB surgió con el objetivo de satisfacer la creciente demanda de conexiones inalámbricas en interiores y de uso doméstico, con bajo coste y alta velocidad. La disponibilidad de un ancho de banda grande, el potencial para alta velocidad de ...

  8. The fast neutron SEU cross section of a 4 Mb SRAM memory

    Energy Technology Data Exchange (ETDEWEB)

    Pereira Junior, Evaldo C.F.; Goncalez, Odair L.; Cruz, Marco Aurelio da; Prado, Adriane Cristina Mendes; Federico, Claudio Antonio; Gaspar, Felipe de Barros, E-mail: claudiofederico@ieav.cta.br, E-mail: odairlelisgoncalez@gmail.com, E-mail: evaldocarlosjr@gmail.com, E-mail: adriane.acm@hotmail.com [Instituto de Estudos Avancados (IEAv/DCTA), Sao Jose dos Campos, SP (Brazil)

    2013-07-01

    The results of a static test of single event upset (SEU) produced by fast neutrons on an ISSI 4Mb SRAM memory are reported in this work. To perform the tests, it was built a platform based on a motherboard which is controlled by microprocessor, whose function is to perform the writing, reading and control of the memories under irradiation. The irradiation was performed with a set of 8 {sup 241}Am-Be neutrons source in a quasi-isotropic incidence. The SEU cross was calculated from the accumulated bit flip count. (author)

  9. Evaluation of Microstructure and Mechanical Property of FSW Welded MB3 Magnesium Alloy

    Institute of Scientific and Technical Information of China (English)

    WANG Kuai-she; SHEN Yang; YANG Xi-rong; WANG Xun-hong; XU Ke-wei

    2006-01-01

    An experiment was carried out on the friction stir welding of MB3 magnesium alloy to determine welding parameters for obtaining an excellent weld appearance without void, cracking, or distortion. Frictional heat and plastic flow created fine and equiaxed grains in the weld nugget, and the elongated and recovered grains in the thermomechanically affected zone (TMAZ). The grains in the heat affected zone (HAZ) grow slightly. The mechanical property results show that maximum joint tensile strength can reach 97.2% of the parent material, which is stronger than that of fusion joints; and the failure almost occurs in the heat affected zone.

  10. An alternative experimental approach for subcritical configurations of the IPEN/MB-01 nuclear reactor

    Science.gov (United States)

    Gonnelli, E.; Lee, S. M.; Pinto, L. N.; Landim, H. R.; Diniz, R.; Jerez, R.; dos Santos, A.

    2015-07-01

    This work presents an alternative approach for the reactivity worth experiments analysis in the IPEN/MB-01 reactor considering highly subcritical arrays. In order to reach the subcritical levels, the removal of a specific number of fuel rods is proposed. Twenty three configurations were carried out for this purpose. The control bank insertion experiment was used only as reference for the fuel rod experiment and, in addition, the control banks were maintained completely withdrawn during all the fuel rods experiment. The theoretical simulation results using the MCNP5 code and the ENDF/B-VII.0 library neutron data are in a very good agreement to experimental results.

  11. IPEN-MB-1 critical facility calculation using CONDOR-CITVAP codes

    Energy Technology Data Exchange (ETDEWEB)

    Villarino, Eduardo Anibal [INVAP SE, San Carlos de Bariloche, Rio Negro (Argentina)

    1997-12-31

    The IPEN-MB-1 critical facility was calculated using the INVAP calculation line CONDOR-CITVAP. The calculated parameters are the reactivity excess of the core, the critical position of the control rods, and the reactivity curves of the control rods. The cell models (CONDOR v1.3) and core models (CITVAP v3.1) are presented. A numerical analysis of the with different calculation parameters is also presented. The analyzed parameters were: model, energy groups and number of spatial meshes. An additional comparison with the MCNP code were performed and it presented in this paper. (author) 16 refs., 3 figs., 12 tabs.

  12. HS-SPME/GC/MS法测定水中土臭素和2-甲基异崁醇的条件优化%Determination of GSM and 2-MIB in natural water by headspace solid-phase micro-extraction coupled with gas chromatography/mass spectrometry

    Institute of Scientific and Technical Information of China (English)

    彭世富; 丁震; 夏卫文; 郑浩; 夏玉婷; 陈晓东

    2013-01-01

    目的:探索顶空固相微萃取-气相色谱/质谱法(HS-SPME/GC/MS)测定水中嗅味物质土臭素(GSM)和2-甲基异嵌醇(2-MIB)的最佳实验条件.方法:应用L32(49)正交表安排顶空固相微萃取-气相色谱/质谱法测定水中嗅味物质时的萃取温度、萃取时间、搅拌子转速、盐浓度、解吸时间和解吸温度并进行实验,优化各项操作参数.结果:顶空固相微萃取-气相色谱/质谱法测定水中嗅味物质的优化后实验条件是:萃取温度和解吸温度分别为65℃和260℃,萃取时间和解吸时间分别是40 min和7 min,搅拌子转速是600 r/min,盐浓度为30%(W/V).分别测定高低加标样,回收率为91%~107%,检出限(S/N=3)为0.04 ng/L,回收率为91%~104%,检出限(S/N=3)为0.13 ng/L.结论:通过正交实验设计优化实验条件,所建立的分析方法准确、简便、可靠,符合水中嗅味物质的测定要求.%Objective:To optimize the conditions for determination of the earthy and musty odors geosmin,2-methylisoborneol (2-MIB) in natural water by hcadspace solid-phase micro-extraction (SPME) coupled with gas chromatography/mass spectrometry and to establish a proposed method for determining the typical odor compounds in water.Methods:By applying the orthogonal experiment design L32(49) to arrange the determination conditions,several parameters of the extraction and desorption were studied and optimized (such as extraction temperature,desorption temperature,extraction time,ionic strength and rotate-speed),and some other parameters (such as correlation coefficient,limits of detection,RSD and recovery) were applied to evaluate effect of this method.Results:The optimum temperature of extraction and desorption were 65℃ and 260℃,respectively;time of extraction and desorption were 40 min and 7 min,respectively ;ionic strength,30% (W/V) ; rotate-speed,600 rpm.Under the optimized experimental conditions,limits of detection (S/N=3) were 0.04 and 0.42 ng/L for

  13. Determination of 2 MIB and Geosmin in Water by HS SPME GC MS MS%顶空固相微萃取三重四级杆气质联用法测定水中2甲基异莰醇和土臭素

    Institute of Scientific and Technical Information of China (English)

    侯磊; 俞超; 向华; 陆峰; 汪永喜

    2015-01-01

    该文运用了 HS SPME GC MS MS法测定水中痕量2 MIB和 GSM。当测定范围为2~200 ng / L时,标准曲线的线性相关系数均≥0.9995;该方法的检出限分别为0.59和0.68 ng / L;RSD为3.96%和4.54%;出厂水、管网水和原水的加标回收率为86.3%~113.7%。在藻类大量增殖时期,监测上海地区某水库内、外原水中2 MIB浓度,发现藻细胞数较多的原水中2 MIB浓度也较高;当藻细胞数低于5×106个/ L时,2 MIB浓度低于国标限值(10 ng / L)。此外库内水中2 MIB的产生相对于藻的产生具有一定的滞后性,且两者存在较为一致的变化趋势,佐证了2 MIB为藻类次生代谢产物;GSM的产生则与藻类代谢无明显相关性。%The trace 2 MIB and GSM in water was determined by HS SPME GC MS MS in this paper. When range of determination is 2 ~ 200 ng / L,the correlation coefficient of stand curve is ≥0. 999 5.The limits of detection are 059 and 0. 68 ng / L, respectively. And their RSD are 3. 96% and 4. 54%,respectively. The recovery rates in finished water,network water and raw water are 86. 3% ~ 113. 7% . The concentration of 2 MIB monitored in and outside the Shanghai-based reservoir during the period of algal bloom is higher in the raw water where the algal cell count is higher. Meanwhile,the concentration of 2 MIB is below the limit value (10 ng / L)according to the national standard when the algal cell count is below 5 × 106 Cell/ L. Moreover,the production of 2 MIB lags the algal bloom and the concentration of 2 MIB has a similar variation trend with the algal cell count obviously in the reservoir, which infers that 2 MIB is a kind of algal secondary metabolic product while GSM has not the same pattern apparently.

  14. Construct Validity of Science Motivation and Beliefs Instrument (SLA-MB): A Case study in Sumedang, Indonesia

    Science.gov (United States)

    Rachmatullah, A.; Octavianda, R. P.; Ha, M.; Rustaman, N. Y.; Diana, S.

    2017-02-01

    Along with numerous instruments developed and used in science education researches, some of those instruments have been translated to local language in the country where the instruments were used. Most of researchers that used those translated instruments did not report the quality of those translated instruments. One of the instruments is the Scientific Literacy Assessment (SLA) including the Science Motivation and Beliefs (SLA-MB) as part of the SLA. In this study, the SLA-MB has been translated into Indonesian Language (Bahasa). The purpose of this study is to investigate the SLA-MB instrument that has been translated to Indonesian language from the view of dimensionality, reliability, item quality and differential item functioning (DIF) based on IRT-Rasch analysis. We used Conquest and Winstep as the program for IRT-Rasch analysis. We employed quantitative research method with school-survey on this study. Research subjects are 223 Indonesian Middle school students (age 13-16), with 64 boys and 159 girls. IRT-Rasch analysis of the SLA-MB Indonesian version indicated that a three-dimensional model fit significantly better than one-dimension model, and the reliability of each dimensions are about 0.60 to 0.82. As well as those findings, fit values of all items are acceptable, moreover we found no DIF for all of the SLA-MB items. Overall, our study suggests that Indonesian version of SLA-MB is acceptable to be implemented as research instrument conducted in Indonesia.

  15. An ex vivo comparison of detection ability of three methods in discovering of MB2 canal in maxillary molars

    Directory of Open Access Journals (Sweden)

    Ghorbanzadeh A.

    2009-11-01

    Full Text Available "nBackground and Aim: A considerable percentage of failure in Endodontic treatments in maxillary molars is attributed to undiscovered second mesiobuccal canal (MB2.There are different methods for discovering and accessing to this canal. The purpose of this ex vivo study was to compare the detection ability of three methods (direct look, fiberoptic loup and surgical microscope to find MB2 after troughing with ultrasonic. "nMaterials and Methods: In this experimental study, we selected 90 extracted maxillary molars (45 first and 45 second molars in which after access cavity preparation MB2 canal was not discovered by direct vision and endodontic explorer. They were divided into 3 groups (n=30. The dentinal shelf between mesiobuccal and palatal canals was eliminated by an endodontic ultrasonic tip (troughing. After that, first group was searched by direct vision, second group by a loup and fiberoptic light and third group by dental operating microscope. Data were analyzed, specificity and sensitivity were calculated. "nResults: The results showed that 21%, 61%, and 92% of MB2 canals after troughing was found by direct vision, fiberoptic loup, and surgical microscope, respectively. "nConclusion: Based on the results of this study, surgical microscope and loup with fiberoptic are preferred methods for discovering MB2 canal. Troughing with ultrasonic can help find MB2 canal in all methods.

  16. Cerebral hemorrhage increases plasma concentrations of noradrenalin and creatine kinase MB fraction with induction of cardiomyocyte damage in rats.

    Science.gov (United States)

    Liang, Xiao-Min; Chen, Jia; Zhang, Tao; Liu, Juan; Jiang, Xiao-Jiang; Xu, Zhi-Qiang

    2014-12-01

    The incidence of cardiac damage is high during acute cerebral hemorrhage. The animal data on the relationship between cerebral apoplexy and cardiac damage are lacking. Thus, the aim of the study was to evaluate the effects of cerebral hemorrhage on plasma concentrations of monoamine transmitter noradrenalin (NA), creatine kinase muscle and brain (CK-MB) isoenzyme fraction, and cardiomyocyte changes in the rat model. In this study, 140 Wistar rats were randomly and equally divided into experimental and control groups, and collagenase was injected into the right caudate nucleus to induce cerebral hemorrhage in the experimental group. Plasma NA was analyzed using high-performance liquid chromatography with electrochemical detection and serum CK-MB was measured by enzyme reaction rate method. We found that both NA and CK-MB were elevated (p MB concentrations reached peak levels at 24 h which were found to be 3.52 ± 0.06 μg/L and 5.47 ± 0.49 μkat/L, respectively. Thereafter, NA and CK-MB concentrations decreased gradually. Plasma NA declined to the preoperative level (1.66 ± 0.03 μg/L) at 72 h, while CK-MB level (2.71 ± 0.17 μkat/L) was found to be still higher than its preoperative level. It was, therefore, concluded that plasma NA might be involved in the induction and development of cardiomyocytes damage during cerebral hemorrhage.

  17. Out-of-core detectors experiments in IPEN/MB-01 reactor; Experimento sobre detetores out-of-core no reator IPEN/MB-01

    Energy Technology Data Exchange (ETDEWEB)

    Abe, Alfredo Y.; Fuga, Rinaldo; Mendonca, Arlindo Gilson; Moreira, Joao M.L.; Angioletto, Elcio [Centro Tecnologico da Marinha em Sao Paulo (CTMSP), SP (Brazil); Fanaro, Leda Cristina C.B.; Jerez, Rogerio; Coelho, Paulo R. Pinto; Santos, Adimir dos; Silva, Graciete S. de A. e; Diniz, Ricardo [Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil)

    2000-07-01

    In order to study the response of out-of-core detectors, 16 stainless steel plates, with 0.5 cm thickness, were placed at the core-reflector interface of the IPEN/MB-01 reactor. BF{sub 3}, {sup 10} B and Au foil detectors were localized beyond the stainless steel plates in 7 different positions, one of them outside the moderator tank of the reactor for simulating a true PWR out-of-core detector. Calculations were performed for comparison with the experimental results with the TORT code, a three-dimensional transport theory discrete ordinate code. The experiment model utilized 16 energy groups, X-Y{sub Z} geometry, S{sub 16} discrete ordinates and P{sub 3} cross-sections. The obtained results showed a good agreement between measured and calculated reaction rates in Au foils. The larger discrepancy occurred for the case with 16 stainless steel with a 2,2% deviation. For position 7, outside of the moderator tank, the neutron flux was so low that it could not active the Au foils for the reaction rate measurements. (author)

  18. The study on the concentration characteristics of 2-MIB and geosmin in downstream water of Minjian-river%闽江下游水域中2--甲基异茨醇和土臭素含量变化特征研究

    Institute of Scientific and Technical Information of China (English)

    薛芝敏; 吴燕芳

    2012-01-01

      本研究建立了一种基于固相萃取和气相色谱氢火焰检测技术(GC-FID)分析检测水样中微量2--甲基异茨醇和土臭素的方法。所建立的方法具有简单、可靠、灵敏等优点。方法的检测限分别为5ng/L(2--甲基异茨醇)和2ng/L(土臭素),5次测定的相对标准偏差小于5%,回收率大于90%。利用所建立的方法,我们详细研究了福建闽江下游水样中2--甲基异茨醇和土臭素的浓度变化特征。%  A simple and reliable method for the analysis of 2-methylisoborneol (2-MIB) and geosmin in river water using gas chromatography-flame ionization detector (GC-FID) together with solid phase extraction (SPE) was developed. With the help of this method, we have studied the variation of the concentrations of 2-MIB and geosmin in river water collected from Minjiang-river at Fuzhou, Fujian of China in detail with detection limits of 2 ng/L (2-MIB) and 5 ng/L (geosmin), a relative standard deviation (RSD)90%.

  19. Development and Evaluation of a New Creatine Kinase MB Mass Determination Assay Using a Latex Agglutination Turbidimetric Immunoassay with an Automated Analyzer.

    Science.gov (United States)

    Hoshino, Tadashi; Hanai, Kazuma; Tanimoto, Kazuhito; Nakayama, Tomohiro

    2016-01-01

    The diagnosis of myocardial infraction (MI) in patients presenting to the emergency department represents a clinical challenge. It is known that creatine kinase-MB isoenzyme (CK-MB) is present in soluble cell fractions of cardiac muscle, and injury to those cells results in an increase of CK-MB in the blood. Therefore, CK-MB is a suitable clinical biomarker of myocardial infraction. To measure CK-MB mass rapidly and easily, we developed the new reagent 'L-type Wako CK-MB mass' (L-CK-MB mass) for the latex agglutination turbidimetric immunoassay method. Using a Hitachi LABOSPECT 008, we evaluated the performance of this assay as a method for quantifying CK-MB mass, and we compared the measurement of the serum CK-MB mass concentration with this assay to that obtained using an electrochemiluminescence immunoassay (ECLIA). A dilution test showed linearity from 5 μg/L to 190 μg/L, and the limit of quantification of the L-CK-MB mass assay was 3.0 μg/L. The within-run CV and between-day CV were 1.0 - 4.5% and 1.8 - 4.4%, respectively. Serum CK-MB mass concentration determined using the L-CK-MB mass assay was reliably and strongly correlated with that determined using ECLIA (n = 163, r = 0.999, y = 0.977x + 0.307). The L-CK-MB mass assay is able to specifically determine CK-MB mass and is a very useful method for the accurate measurement of CK-MB mass for routine clinical analyses.

  20. A Novel Reconfigurable MB-OFDM UWB LNA Using Programmable Current Reuse

    Directory of Open Access Journals (Sweden)

    Ahmed Ragheb

    2013-01-01

    Full Text Available This paper presents a design of a reconfigurable low noise amplifier (LNA for multiband orthogonal frequency division multiplexing (MB-OFDM ultra wideband (UWB receivers. The proposed design is divided into three stages; the first one is a common gate (CG topology to provide the input matching over a wideband. The second stage is a programmable circuit to control the mode of operation. The third stage is a current reuse topology to improve the gain, flatness and consume lower power. The proposed LNA is designed using 0.18 μm CMOS technology. This LNA has been designed to operate in two subbands of MB-OFDM UWB, UWB mode-1 and mode-3, as a single or concurrent mode. The simulation results exhibit the power gain up to 17.35, 18, and 11 dB for mode-1, mode-3, and concurrent mode, respectively. The NF is 3.5, 3.9, and 6.5 and the input return loss is better than −12, −13.57, and −11 dB over mode-1, mode-3, and concurrent mode, respectively. This design consumes 4 mW supplied from 1.2 V.

  1. The 2009 earthquake, magnitude mb 4.8, in the Pantanal Wetlands, west-central Brazil.

    Science.gov (United States)

    Dias, Fábio L; Assumpção, Marcelo; Facincani, Edna M; França, George S; Assine, Mario L; Paranhos, Antônio C; Gamarra, Roberto M

    2016-09-01

    The main goal of this paper is to characterize the Coxim earthquake occurred in June 15th, 2009 in the Pantanal Basin and to discuss the relationship between its faulting mechanism with the Transbrasiliano Lineament. The earthquake had maximum intensity MM V causing damage in farm houses and was felt in several cities located around, including Campo Grande and Goiânia. The event had an mb 4.8 magnitude and depth was 6 km, i.e., it occurred in the upper crust, within the basement and 5 km below the Cenozoic sedimentary cover. The mechanism, a thrust fault mechanism with lateral motion, was obtained by P-wave first-motion polarities and confirmed by regional waveform modelling. The two nodal planes have orientations (strike/dip) of 300°/55° and 180°/55° and the orientation of the P-axis is approximately NE-SW. The results are similar to the Pantanal earthquake of 1964 with mb 5.4 and NE-SW compressional axis. Both events show that Pantanal Basin is a seismically active area, under compressional stress. The focal mechanism of the 1964 and 2009 events have no nodal plane that could be directly associated with the main SW-NE trending Transbrasiliano system indicating that a direct link of the Transbrasiliano with the seismicity in the Pantanal Basin is improbable.

  2. MB-OFDM-UWB Based Wireless Multimedia Sensor Networks for Underground Coalmine: A Survey

    Science.gov (United States)

    Han, Ruisong; Yang, Wei; You, Kaiming

    2016-01-01

    Safety production of coalmines is a task of top priority which plays an important role in guaranteeing, supporting and promoting the continuous development of the coal industry. Since traditional wireless sensor networks (WSNs) cannot fully meet the requirements of comprehensive environment monitoring of underground coalmines, wireless multimedia sensor networks (WMSNs), enabling the retrieval of multimedia information, are introduced to realize fine-grained and precise environment surveillance. In this paper, a framework for designing underground coalmine WMSNs based on Multi-Band Orthogonal Frequency-Division Multiplexing Ultra-wide Band (MB-OFDM-UWB) is presented. The selection of MB-OFDM-UWB wireless transmission solution is based on the characteristics of underground coalmines. Network structure and design challenges are analyzed first, which is the foundation for further discussion. Then, key supporting technologies and open research areas in different layers are surveyed, and we provide a detailed literature review of the state of the art strategies, algorithms and general solutions in these issues. Finally, other research issues like localization, information processing, and network management are discussed. PMID:27999258

  3. Genetic redundancy is prevalent within the 6.7 Mb Sinorhizobium meliloti genome.

    Science.gov (United States)

    diCenzo, George C; Finan, Turlough M

    2015-08-01

    Biological pathways are frequently identified via a genetic loss-of-function approach. While this approach has proven to be powerful, it is imperfect as illustrated by well-studied pathways continuing to have missing steps. One potential limiting factor is the masking of phenotypes through genetic redundancy. The prevalence of genetic redundancy in bacterial species has received little attention, although isolated examples of functionally redundant gene pairs exist. Here, we made use of a strain of Sinorhizobium meliloti whose genome was reduced by 45 % through the complete removal of a megaplasmid and a chromid (3 Mb of the 6.7 Mb genome was removed) to begin quantifying the level of genetic redundancy within a large bacterial genome. A mutagenesis of the strain with the reduced genome identified a set of transposon insertions precluding growth of this strain on minimal medium. Transfer of these mutations to the wild-type background revealed that 10-15 % of these chromosomal mutations were located within duplicated genes, as they did not prevent growth of cells with the full genome. The functionally redundant genes were involved in a variety of metabolic pathways, including central carbon metabolism, transport, and amino acid biosynthesis. These results indicate that genetic redundancy may be prevalent within large bacterial genomes. Failing to account for redundantly encoded functions in loss-of-function studies will impair our understanding of a broad range of biological processes and limit our ability to use synthetic biology in the construction of designer cell factories.

  4. PERFORMANCE ANALYSIS OF PILOT BASED CHANNEL ESTIMATION TECHNIQUES IN MB OFDM SYSTEMS

    Directory of Open Access Journals (Sweden)

    M. Madheswaran

    2011-12-01

    Full Text Available Ultra wideband (UWB communication is mainly used for short range of communication in wireless personal area networks. Orthogonal Frequency Division Multiplexing (OFDM is being used as a key physical layer technology for Fourth Generation (4G wireless communication. OFDM based communication gives high spectral efficiency and mitigates Inter-symbol Interference (ISI in a wireless medium. In this paper the IEEE 802.15.3a based Multiband OFDM (MB OFDM system is considered. The pilot based channel estimation techniques are considered to analyze the performance of MB OFDM systems over Liner Time Invariant (LTI Channel models. In this paper, pilot based Least Square (LS and Least Minimum Mean Square Error (LMMSE channel estimation technique has been considered for UWB OFDM system. In the proposed method, the estimated Channel Impulse Responses (CIRs are filtered in the time domain for the consideration of the channel delay spread. Also the performance of proposed system has been analyzed for different modulation techniques for various pilot density patterns.

  5. Fatigue Properties of Welded Butt Joint and Base Metal of MB8 Magnesium Alloy

    Directory of Open Access Journals (Sweden)

    Ying-xia YU

    2016-09-01

    Full Text Available The fatigue properties of welded butt joint and base metal of MB8 magnesium alloy were investigated. The comparative fatigue tests were carried out using EHF-EM200K2-070-1A fatigue testing machine for both welded butt joint and base metal specimens with the same size and shape. The fatigue fractures were observed and analyzed by a scanning electron microscope of 6360 LA type. The experimental results show that the fatigue performance of the welded butt joint of MB8 magnesium alloy is sharply decreased. The conditional fatigue limit (1×107 of base metal and welded butt joint is about 69.41 and 32.76 MPa, respectively. The conditional fatigue limit (1×107 of the welded butt joint is 47.2 % of that of base metal. The main reasons are that the welding can lead to stress concentration in the weld toe area, tensile welding residual stress in the welded joint, as well as grain coarsening in the welding seam. The cleavage steps or quasi-cleavage patterns present on the fatigue fracture surface, indicating the fracture type of the welded butt joint belongs to a brittle fracture.DOI: http://dx.doi.org/10.5755/j01.ms.22.3.9132

  6. Cadmium effects on p38/MAPK isoforms in MDA-MB231 breast cancer cells.

    Science.gov (United States)

    Casano, Caterina; Agnello, Maria; Sirchia, Rosalia; Luparello, Claudio

    2010-02-01

    Emerging evidence seems to indicate that the heavy metal cadmium (Cd) is able to regulate gene expression, drastically affecting the pattern of transcriptional activity in normal and pathological eukaryotic cells, also affecting intracellular signalization events. Human p38 is a family of mitogen-activated protein kinases consisting of four isoforms (alpha, beta, gamma and delta) which mediate signal transduction cascades controlling several aspects of cell physiology. In this study we examined whether exposure of MDA-MB231 tumor cells from the human breast to Cd may exert some effect on p38 isoform expression and accumulation, as well as on p38 activation. Employing a combination of proliferation tests, conventional and semiquantitative multiplex (SM)-polymerase chain reaction (PCR) and Western blot assays, we report that the treatment of breast cancer cells with 5 microM CdCl(2) induces a diversified modulation of the transcription patterns of p38 isoform genes and of the accumulation of the related protein products, which are, on the other hand, also affected by alpha and beta isoform functional inactivation induced by SB203580. Our findings suggest the existence of so far unexplored mechanisms of gene regulation in our model system and validate that MDA-MB231 cell line is a suitable in vitro model for further and more detailed studies on the intracellular mechanisms underlying the control of p38 expression, synthesis and activation in mammary tumor cells exposed to different stresses.

  7. MB-OFDM-UWB Based Wireless Multimedia Sensor Networks for Underground Coalmine: A Survey.

    Science.gov (United States)

    Han, Ruisong; Yang, Wei; You, Kaiming

    2016-12-16

    Safety production of coalmines is a task of top priority which plays an important role in guaranteeing, supporting and promoting the continuous development of the coal industry. Since traditional wireless sensor networks (WSNs) cannot fully meet the requirements of comprehensive environment monitoring of underground coalmines, wireless multimedia sensor networks (WMSNs), enabling the retrieval of multimedia information, are introduced to realize fine-grained and precise environment surveillance. In this paper, a framework for designing underground coalmine WMSNs based on Multi-Band Orthogonal Frequency-Division Multiplexing Ultra-wide Band (MB-OFDM-UWB) is presented. The selection of MB-OFDM-UWB wireless transmission solution is based on the characteristics of underground coalmines. Network structure and design challenges are analyzed first, which is the foundation for further discussion. Then, key supporting technologies and open research areas in different layers are surveyed, and we provide a detailed literature review of the state of the art strategies, algorithms and general solutions in these issues. Finally, other research issues like localization, information processing, and network management are discussed.

  8. Hazardous Traffic Event Detection Using Markov Blanket and Sequential Minimal Optimization (MB-SMO

    Directory of Open Access Journals (Sweden)

    Lixin Yan

    2016-07-01

    Full Text Available The ability to identify hazardous traffic events is already considered as one of the most effective solutions for reducing the occurrence of crashes. Only certain particular hazardous traffic events have been studied in previous studies, which were mainly based on dedicated video stream data and GPS data. The objective of this study is twofold: (1 the Markov blanket (MB algorithm is employed to extract the main factors associated with hazardous traffic events; (2 a model is developed to identify hazardous traffic event using driving characteristics, vehicle trajectory, and vehicle position data. Twenty-two licensed drivers were recruited to carry out a natural driving experiment in Wuhan, China, and multi-sensor information data were collected for different types of traffic events. The results indicated that a vehicle’s speed, the standard deviation of speed, the standard deviation of skin conductance, the standard deviation of brake pressure, turn signal, the acceleration of steering, the standard deviation of acceleration, and the acceleration in Z (G have significant influences on hazardous traffic events. The sequential minimal optimization (SMO algorithm was adopted to build the identification model, and the accuracy of prediction was higher than 86%. Moreover, compared with other detection algorithms, the MB-SMO algorithm was ranked best in terms of the prediction accuracy. The conclusions can provide reference evidence for the development of dangerous situation warning products and the design of intelligent vehicles.

  9. Hazardous Traffic Event Detection Using Markov Blanket and Sequential Minimal Optimization (MB-SMO).

    Science.gov (United States)

    Yan, Lixin; Zhang, Yishi; He, Yi; Gao, Song; Zhu, Dunyao; Ran, Bin; Wu, Qing

    2016-07-13

    The ability to identify hazardous traffic events is already considered as one of the most effective solutions for reducing the occurrence of crashes. Only certain particular hazardous traffic events have been studied in previous studies, which were mainly based on dedicated video stream data and GPS data. The objective of this study is twofold: (1) the Markov blanket (MB) algorithm is employed to extract the main factors associated with hazardous traffic events; (2) a model is developed to identify hazardous traffic event using driving characteristics, vehicle trajectory, and vehicle position data. Twenty-two licensed drivers were recruited to carry out a natural driving experiment in Wuhan, China, and multi-sensor information data were collected for different types of traffic events. The results indicated that a vehicle's speed, the standard deviation of speed, the standard deviation of skin conductance, the standard deviation of brake pressure, turn signal, the acceleration of steering, the standard deviation of acceleration, and the acceleration in Z (G) have significant influences on hazardous traffic events. The sequential minimal optimization (SMO) algorithm was adopted to build the identification model, and the accuracy of prediction was higher than 86%. Moreover, compared with other detection algorithms, the MB-SMO algorithm was ranked best in terms of the prediction accuracy. The conclusions can provide reference evidence for the development of dangerous situation warning products and the design of intelligent vehicles.

  10. BaO/Al2O3/NiAl(110) Model NOx Storage Materials. The effect of BaO film thickness on the amorphous-to-crystalline Ba(NO3)2 phase transition

    Energy Technology Data Exchange (ETDEWEB)

    Yi, Cheol-Woo W. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Szanyi, Janos [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2008-12-18

    The reaction of NO2 with BaO (0.15 – 2 ML and > 30 ML)/Al2O3(12 ML)/NiAl(110) model NOx storage materials was studied. A thick (~12 ML), ordered Al2O3 film was prepared as the support oxide on a NiAl(110) substrate in order to minimize the effect of the intermixing between the two oxide phases (BaO and Al2O3) on the NOx chemistry of BaO. The growth of a thick alumina film, prepared by atomic oxygen deposition onto NiAl(110), follows a layer-by-layer growth mode and the resulting film is much more stable when exposed to NO2 than the ultra-thin alumina films studied before. The interaction of NO2 with the model NOx storage systems at low coverages of BaO show fundamentally different behaviors from a thick BaO film, as nitrite species form at low exposures of NO2, followed by nitrate formation at high NO2 exposures. In contrast, on the thick BaO layer nitrite-nitrate ion pairs form at 300 K under UHV conditions (PNO2 ~ 1 x 10-9 Torr). However, at elevated NO2 pressures (≥ 1 x 10-5 Torr) the thick BaO film is gradually converted into amorphous Ba(NO3)2 at 300 K. Raising the temperature of the samples with ΘBaO > 1 ML after NO2 exposure (in the absence of gas phase NO2) leads to the phase transformation of the amorphous Ba(NO3)2 layer into crystalline Ba(NO3)2 particles in the temperature range of 500 – 600 K. No phase transformation is observed in samples with ΘBaO < 1 ML.

  11. 王暴魁教授寒热并用治疗急性咽痛1例%Experience in treating acute sore throat of professor WANG Bao-kui by using both of cold and heat therapy

    Institute of Scientific and Technical Information of China (English)

    谢璇

    2012-01-01

    咽痛是临床常见症状之一,文章通过1例急性咽痛医案,具体分析王暴魁教授以麻黄附子细辛汤寒热并用治疗本病的辨证思路,详辨其常选用半夏、牡蛎等遣方用药的特点,丰富临床急性咽痛的辨证治疗思路.%Sore throat is a common clinical symptom. This article detailed analysed the therapy of using both cold and heat by Mahuangfuzixixin Decoction to treat acute sore throat with one specific case discussion, and professor WANG Bao-kui's experience in traditional Chinese medicine usage as Banxia, Muli, and so on. From the analysis, the treatment methodology of acute sore throat could be refined.

  12. 小区合并技术在西宝高速铁路中的应用研究%The application research of village merging technology in Xi-Bao high speed railway

    Institute of Scientific and Technical Information of China (English)

    王波

    2016-01-01

    This paper introduced the principle of village merging technology,analyzed the advantage of RRU multi-site community,elaborated the application scheme of village merging technology in Xi-Bao high speed railway,pointed out that village merging technology had important role to improve the high speed rail network coverage quality,improve customer satisfaction,improve operator brand image.%介绍了小区合并技术的原理,分析了RRU多站点共小区的优点,阐述了小区合并技术在西宝高速铁路中的应用方案,指出小区合并技术对改善高铁网络覆盖质量、提高客户满意度、提升运营商品牌形象有重要作用。

  13. Novel κ-opioid receptor agonist MB-1C-OH produces potent analgesia with less depression and sedation.

    Science.gov (United States)

    Zhang, Le-sha; Wang, Jun; Chen, Jian-chun; Tao, Yi-min; Wang, Yu-hua; Xu, Xue-jun; Chen, Jie; Xu, Yun-gen; Xi, Tao; Hu, Xiao-wu; Wang, Yu-jun; Liu, Jing-gen

    2015-05-01

    To characterize the pharmacological profiles of a novel κ-opioid receptor agonist MB-1C-OH. [(3)H]diprenorphine binding and [(35)S]GTPγS binding assays were performed to determine the agonistic properties of MB-1C-OH. Hot plate, tail flick, acetic acid-induced writhing, and formalin tests were conducted in mice to evaluate the antinociceptive actions. Forced swimming and rotarod tests of mice were used to assess the sedation and depression actions. In [(3)H]diprenorphine binding assay, MB-1C-OH did not bind to μ- and δ-opioid receptors at the concentration of 100 μmol/L, but showed a high affinity for κ-opioid receptor (Ki=35 nmol/L). In [(35)S]GTPγS binding assay, the compound had an Emax of 98% and an EC50 of 16.7 nmol/L for κ-opioid receptor. Subcutaneous injection of MB-1C-OH had no effects in both hot plate and tail flick tests, but produced potent antinociception in the acetic acid-induced writhing test (ED50=0.39 mg/kg), which was antagonized by pretreatment with a selective κ-opioid receptor antagonist Nor-BNI. In the formalin test, subcutaneous injection of MB-1C-OH did not affect the flinching behavior in the first phase, but significantly inhibited that in the second phase (ED50=0.87 mg/kg). In addition, the sedation or depression actions of MB-1C-OH were about 3-fold weaker than those of the classical κ agonist (-)U50,488H. MB-1C-OH is a novel κ-opioid receptor agonist that produces potent antinociception causing less sedation and depression.

  14. Pharmacokinetic profile and quantitation of protection against soman poisoning by the antinicotinic compound MB327 in the guinea-pig.

    Science.gov (United States)

    Price, Matthew E; Docx, Cerys J; Rice, Helen; Fairhall, Sarah J; Poole, Sarah J C; Bird, Michael; Whiley, Luke; Flint, Daniel P; Green, A Christopher; Timperley, Christopher M; Tattersall, John E H

    2016-02-26

    Current organophosphorus nerve agent medical countermeasures do not directly address the nicotinic effects of poisoning. A series of antinicotinic bispyridinium compounds has been synthesized in our laboratory and screened in vitro. Their actions can include open-channel block at the nicotinic receptor which may contribute to their efficacy. The current lead compound from these studies, MB327 1,1'-(propane-1,3-diyl)bis(4-tert-butylpyridinium) as either the diiodide (I2) or dimethanesulfonate (DMS) has been examined in vivo for efficacy against nerve agent poisoning. MB327 I2 (0-113mgkg(-1)) or the oxime HI-6 DMS (0-100mgkg(- 1)), in combination with atropine and avizafone (each at 3mgkg(-1)) was administered to guinea-pigs 1min following soman poisoning. Treatment increased the LD50 of soman in a dose-dependent manner. The increase was statistically significant (p<0.01) at the 33.9mgkg(-1) (MB327) or 30mgkg(-1) (HI-6) dose with a comparable degree of protection obtained for both compounds. Following administration of 10mgkg(-1) (i.m.), MB327 DMS reached plasma Cmax of 22μM at 12min with an elimination t1/2 of 22min. In an adverse effect study, in the absence of nerve agent poisoning, a dose of 100mgkg(-1) or higher of MB327 DMS was lethal to the guinea-pigs. A lower dose of MB327 DMS (30mgkg(-1)) caused flaccid paralysis accompanied by respiratory impairment. Respiration normalised by 30min, although the animals remained incapacitated to 4h. MB327 or related compounds may be of utility in treatment of nerve agent poisoning as a component of therapy with atropine, anticonvulsant and oxime, or alternatively as an infusion under medical supervision.

  15. Four-character Loanwords from Japanese in the Chinese Newspaper QingYiBao(1898-1901)%《清议报》中的四字日语借词

    Institute of Scientific and Technical Information of China (English)

    朱京伟

    2013-01-01

    The period around which QingYiBao began to boom coincides with the increase of unidiomatic four-character words in Chinese. For this reason, the importance of QingYiBao as a source for research into historical lexicology cannot be ignored. In addition, former studies of loanwords from Japanese mainly focused on distinguishing and sorting out two-character Japanese loanwords, while three-character and four-character Japanese loanwords were paid little attention, and related disquisitions are also rare. In the view of the above-mentioned facts, this article takes the four-character words in QingYiBaoas the object of study, distinguishes loanwords from the Japanese origin, and summarizes related study methods.%《清议报》(1898-1901)前后正是汉语中非熟语类四字词开始增多的时段,其作为词汇史研究资料的重要性不容忽视。同时,以往在研究日语借词时,总是把重点放在辨别与整理二字日语借词方面,而对三字、四字形式的日语借词则关注不多,很少有人对此进行专题讨论。有鉴于此,本文将以《清议报》中四字词为研究对象,在辨别日语借词的同时,也对相关的研究方法进行归纳。

  16. Market and economic analysis of "Sleep AnBao" electronic products%“睡眠安宝”电子产品的市场与经济分析

    Institute of Scientific and Technical Information of China (English)

    王晨; 张开生

    2013-01-01

    The "sleep AnBao" can monitor the pulse signal of the sleeper. Once the system detects a sudden illness, you can call or send SMS automatically through wireless communication module and GSM module for emergency help, such as 120 emergency center, friends and family. At the same time, the system can automatically open the electric door lock, so as to facilitate the implementation of the rescue. The product solves mainly the night time rescue problems. By market analysis and economic analysis of the " sleep AnBao" electronic products, show that the product of the operational scheme is feasible.%“睡眠安宝”产品可以对睡眠者的脉搏信号进行监测,一旦系统检测到突发疾病,便可通过无线通信模块及GSM模块,自动拨打电话或发送短信进行紧急报警求救.求救对象可以是120急救中心、亲朋好友等.在报警的同时,系统可自动打开电动门锁,极大地方便了相关人员实施救治.该产品主要解决夜间突发疾病的救助问题,通过对“睡眠安宝”电子产品的市场分析与经济分析,表明该产品的运营方案是切实可行的.

  17. Characterization of a novel PTEN mutation in MDA-MB-453 breast carcinoma cell line

    Directory of Open Access Journals (Sweden)

    Singh Gobind

    2011-11-01

    Full Text Available Abstract Background Cowden Syndrome (CS patients with germ line point mutations in the PTEN gene are at high risk for developing breast cancer. It is believed that cells harboring these mutant PTEN alleles are predisposed to malignant conversion. This article will characterize the biochemical and biological properties of a mutant PTEN protein found in a commonly used metastatic breast cancer cell line. Methods The expression of PTEN in human breast carcinoma cell lines was evaluated by Western blotting analysis. Cell line MDA-MB-453 was selected for further analysis. Mutation analysis of the PTEN gene was carried out using DNA isolated from MDA-MB-453. Site-directed mutagenesis was used to generate a PTEN E307K mutant cDNA and ectopic expressed in PC3, U87MG, MCF7 and Pten-/- mouse embryo fibroblasts (MEFS. Histidine (His-tagged PTEN fusion protein was generated in Sf9 baculovirus expression system. Lipid phosphatase and ubiquitination assays were carried out to characterize the biochemical properties of PTEN E307K mutant. The intracellular localization of PTEN E307K was determined by subcellular fractionation experiments. The ability of PTEN E307K to alter cell growth, migration and apoptosis was analyzed in multiple PTEN-null cell lines. Results We found a mutation in the PTEN gene at codon 307 in MDA-MB-453 cell line. The glutamate (E to lysine (K substitution rendered the mutant protein to migrate with a faster mobility on SDS-PAGE gels. Biochemically, the PTEN E307K mutant displayed similar lipid phosphatase and growth suppressing activities when compared to wild-type (WT protein. However, the PTEN E307K mutant was present at higher levels in the membrane fraction and suppressed Akt activation to a greater extent than the WT protein. Additionally, the PTEN E307K mutant was polyubiquitinated to a greater extent by NEDD4-1 and displayed reduced nuclear localization. Finally, the PTEN E307K mutant failed to confer chemosensitivity to

  18. Functional Expression of Voltage-Gated Sodium Channels Navl.5 in Human Breast Caner Cell Line MDA-MB-231

    Institute of Scientific and Technical Information of China (English)

    Rui GAO; Jing WANG; Yi SHEN; Ming LEI; Zehua WANG

    2009-01-01

    Voltage-gated sodium channels (VGSCs) are known to be involved in the initiation and progression of many malignancies,and the different subtypes of VGSCs play important roles in the metastasis cascade of many tumors.This study investigated the functional expression of Nav 1.5 and its effect on invasion behavior of human breast cancer cell line MDA-MB-231.The mRNA and pro-tein expression of Navl.5 was detected by real time PCR,Western Blot and immunofluorescence.The effects of Navl.5 on cell proliferation,migration and invasion were respectively assessed by MTT and Transwell.The effects of Nav1.5 on the secretion of matrix metalloproteases (MMPs) by MDA-MB-231 were analyzed by RT-PCR.The over-expressed Navl.5 was present on the membrane of MDA-MB-231 cells.The invasion ability in vitro and the MMP-9 mRNA expression were respec-tively decreased to (47.82±0.53)% and (43.97±0.64)% (P<0.05) respectively in MDA-MB-231 cells treated with VGSCs specific inhibitor tetrodotoxin (TTX) by blocking Navl.5 activity.It was con-eluded that Nav1.5 functional expression potentiated the invasive behavior of human breast cancer cell line MDA-MB-231 by increasing the secretion of MMP-9.

  19. [Inhibitory effects of capsaicin on migration and invasion of breast cancer MDA-MB-231 cells and its mechanism].

    Science.gov (United States)

    Li, Bai-He; Yuan, Lei

    2017-04-25

    The aim of this study was to investigate the effects of capsaicin on migration and invasion of breast cancer MDA-MB-231 cells and its possible mechanism. The MDA-MB-231 cells were incubated in the medium containing different concentrations of capsaicin for 24 h. CCK-8 assay was employed to detect the cell viability. The cell migration and invasion were assessed by wound healing assay and transwell invasion assay, respectively. The protein levels of c-Src, p-c-Src (Tyr416), FAK, p-FAK (Tyr576), Paxillin, p-Paxillin (Tyr118), matrix metalloproteinase 2 (MMP2) and MMP9 in the MDA-MB-231 cells were detected by Western blotting. The mRNA expressions of MMP2 and MMP9 were measured by RT-PCR. The result showed that capsaicin (25 and 50 μmol/L) remarkably reduced the abilities of migration and invasion (P MB-231 cells. These effects of capsaicin were all in dose-dependent manners. These results suggest that capsaicin may suppress the migration and invasion of breast cancer MDA-MB-231 cells by inhibiting the phosphorylations of c-Src, FAK and Paxillin, and down-regulating the mRNA and protein levels of MMP2 and MMP9.

  20. 50 CFR 660.116 - Trawl fishery-observer requirements.

    Science.gov (United States)

    2010-10-01

    ... chip, at least 32 megabytes of RAM, at least 75 megabytes of free hard disk storage, a Windows 9x or NT... requirements for the table. The observer's sampling table must be secured to the floor or wall. (v)...

  1. Comparison of the Lowenstein-Jensen Medium, the Middlebrook 7H10 Medium and MB/BacT for the Isolation of Mycobacterium Tuberculosis (MTB) from Clinical Specimens.

    Science.gov (United States)

    Naveen, G; Peerapur, Basavaraj V

    2012-12-01

    Tuberculosis (TB) is the most common cause of death due to a single infectious agent worldwide in adults. India alone accounts for 30% of the global tuberculosis burden. There is a need for a method of cultivation of mycobacteria that is reliable and economical and has a short turnaround time. The present study was attempted to assess the feasibility of using MB BACT and Middlebrook 7h10(MB7H10) as primary isolation media for mycobacteria. They were compared with the LJ medium, which was the gold standard. Various clinical specimens from a total of 236 clinically suspected cases of TB were studied. All the samples were decontaminated by using the modified Petroff's method. Each sample was subjected to ZN staining and it was simultaneously inoculated onto the LJ medium, the MB7H10 medium and MB BACT. The growth from the cultures were confirmed by ZN staining and they were speciated by using biochemical reactions. Out of the 236 samples which were screened, 116 isolates were obtained. All the 116 were isolated from MB BACT, 82 were isolated from the LJ medium and 62 were isolated from MB7H10. 82 isolates were obtained from MB BACT and the LJ medium, 62 were obtained from MB7H10 and MB BACT, 58 were isolated from LJ and MB7H10 and 58 were isolated from LJ medium, MB7H10 and MB bact. Neither the L J medium nor the Middlebrook 7h 10 medium could isolate mycobacteria exclusively. It showed that the combination of media did not prove to be superior over the use of MB BACT alone. The average isolation time of L J, the MB7H10 medium and MB BACT was 30.81 days, 31.06 days and 18.70 days. MB BACT is a better medium as compared to the L J medium and the MB7H10 medium, both in terms of the number of isolates and the isolation rate. The MB BACT method proved to be a very speedy method and it could isolate mycobacteria 7-10 days earlier as compared to the L J medium and the Middlebrook 7 H10 medium.

  2. Super-resolution mbPAINT for optical localization of single-stranded DNA.

    Science.gov (United States)

    Chen, Jixin; Bremauntz, Alberto; Kisley, Lydia; Shuang, Bo; Landes, Christy F

    2013-10-09

    We demonstrate the application of superlocalization microscopy to identify sequence-specific portions of single-stranded DNA (ssDNA) with sequence resolution of 50 nucleotides, corresponding to a spatial resolution of 30 nm. Super-resolution imaging was achieved using a variation of a single-molecule localization method, termed as "motion blur" point accumulation for imaging in nanoscale topography (mbPAINT). The target ssDNA molecules were immobilized on the substrate. Short, dye-labeled, and complementary ssDNA molecules stochastically bound to the target ssDNA, with repeated binding events allowing super-resolution. Sequence specificity was demonstrated via the use of a control, noncomplementary probe. The results support the possibility of employing relatively inexpensive short ssDNAs to identify gene sequence specificity with improved resolution in comparison to the existing methods.

  3. Decolourisation of Red 5 MB dye by microbes isolated from textile dye effluent.

    Science.gov (United States)

    Subashini, P; Hiranmaiyadav, R; Premalatha, M S

    2010-07-01

    One of the major environmental problems is the presence of dye materials in textile wastewater, which need to be removed before releasing into the environment. Some dyes are toxic and carcinogenic in nature. The discharge of the textile effluent into rivers and lakes leads to higher BOD causing threat to aquatic life. Development of efficient dye degradation requires suitable strain and its use under favorable condition to realize the degradation potential. In this study, three microorganisms were isolated from the Red 5 MB dye containing textile wastewater. They were identified and tested for the dye decolourisation provided with different sugars as carbon source. The percentage of dye decolorized by Bacillus subtilis, Aspergillus flavus and Aspergillus fumigatus were found to be about 40%, 75% and 53.8% respectively.

  4. Cell cycle arrest induced by MPPa-PDT in MDA-MB-231 cells

    Science.gov (United States)

    Liang, Liming; Bi, Wenxiang; Tian, Yuanyuan

    2016-05-01

    Photodynamic therapy (PDT) is a medical treatment using a photosensitizing agent and light source to treat cancers. Pyropheophorbidea methyl ester (MPPa), a derivative of chlorophyll, is a novel potent photosensitizer. To learn more about this photosensitizer, we examined the cell cycle arrest in MDA-MB-231. Cell cycle and apoptosis were measured by flow cytometer. Checkpoints of the cell cycle were measured by western blot. In this study, we found that the expression of Cyclin D1 was obviously decreased, while the expression of Chk2 and P21 was increased after PDT treatment. This study showed that MPPa-PDT affected the checkpoints of the cell cycle and led the cells to apoptosis.

  5. MAGPIE/EGRET Annotation of the 2.9-Mb Drosophila melanogaster Adh Region

    Science.gov (United States)

    Gaasterland, Terry; Sczyrba, Alexander; Thomas, Elizabeth; Aytekin-Kurban, Gulriz; Gordon, Paul; Sensen, Christoph W.

    2000-01-01

    Our challenge in annotating the 2.91-Mb Adh region of the Drosophila melanogaster genome was to identify genetic and genomic features automatically, completely, and precisely within a 6-week period. To do so, we augmented the MAGPIE microbial genome annotation system to handle eukaryotic genomic sequence data. The new configuration required the integration of eukaryotic gene-finding tools and DNA repeat tools into the automatic data collection module. It also required us to define in MAGPIE new strategies to combine data about eukaryotic exon predictions with functional data to refine the exon predictions. At the heart of the resulting new eukaryotic genome annotation system is a reverse comparison of public protein and complementary DNA sequences against the input genome to identify missing exons and to refine exon boundaries. The software modules that add eukaryotic genome annotation capability to MAGPIE are available as EGRET (Eukaryotic Genome Rapid Evaluation Tool). PMID:10779489

  6. 利用MB90f543G实现第一层次的TTCAN

    Institute of Scientific and Technical Information of China (English)

    李传学

    2006-01-01

    在安全性要求极高的一些车辆系统中,基于事件触发的CAN(Controller Area Network)已经不能满足要求.一种采用CAN的解决方法就是将标准CAN扩展成时间触发的协议.TTCAN(Time-Triggered CAN)就是这样一个扩展并且已经成为国际标准协议.本文对TTCAN进行大概的描述并介绍如何用mb90f543G来实现其第一层次扩展的方法.

  7. Source and Fluctuation of Trace Geosmin and 2-MIB in Drinking Water of Shanghai%上海市饮用水中痕量土臭素和二甲基异冰片年变化规律及来源研究

    Institute of Scientific and Technical Information of China (English)

    马晓雁; 高乃云; 李青松; 刘成; 顾国芬

    2008-01-01

    采用液液微萃取-气相色谱/质谱联用测定饮用水中痕量异嗅物质--土臭素(geosmin,GSM)和二甲基异冰片(2-Methylisoborneol,2-MIB或MIB).对上海市饮用水水源以及某水厂工艺出水进行调查发现,土霉昧的来源主要为2-MIB,异嗅问题暴发季节为7~10月,7、8月份达到顶峰,原水中2-MIB的最高检出浓度可达152.82 ng/L.水厂出水中最高检出浓度为97.94 ng/L.初步的来源分析推断2-MIB为水中颤藻的代谢产物.

  8. Is CK-MB isoenzyme useful for diagnosis of cardiac involvement in icteric leptospirosis? CK-MB: útil no diagnóstico de envolvimento cardíaco na leptospirose ictérica

    Directory of Open Access Journals (Sweden)

    Elizabeth Stankiewicz Machado

    1995-10-01

    Full Text Available In the absence of heart failure or cardiogenic shock, cardiac involvement diagnosis in icteric leptospirosis is possible on the basis of abnormal electrocardiograms. As metabolic and electrolytic disorders are frequently seen during acute leptospirosis infection, they may be responsible for some electrocardiograms changes. We conducted a study to assess if creatine phosphokinase isoenzyme determinations are useful in selecting patients with a high cardiac involvement suspicion. Sixty-nine patients were studied prospectively. Ten patients out of 16 with cardiac involvement and 25 without had high CK-MB levels (p>0.05, although mean values of abnormal CK-MB levels were higher in the group with cardiac involvement (pNa ausência de insuficiência cardíaca ou choque cardiogênico, o diagnóstico de envolvimento cardíaco na leptospirose ictérica pode ser baseado em alterações eletrocardiográficas. Devido ao frequente comprometimento multisistêmico da doença, algumas dessas alterações podem ser secundárias a distúrbios metabólicos ou eletrolíticos. Realizou-se um estudo para avaliação do significado da enzima CK-MB elevada em casos suspeitos de envolvimento cardíaco. Sessenta e nove pacientes com leptospirose ictérica foram estudados prospectivamente. Dez dos 16 casos com envolvimento cardíaco e 25 dos 53 pacientes sem envolvimento cardíaco tiveram CK-MB elevada (p>0,05. O valor médio de CK-MB foi maior no grupo com envolvimento cardíaco (p<0,05. Nossa análise indica que a presença de CK-MB elevada no curso de leptospirose ictérica não é indicadora de envolvimento cardíaco.

  9. MB8镁合金植酸转化膜的制备及性能%Preparation and Properties of Phytic Acid Conversion Coating on MB8 Magnesium Alloy

    Institute of Scientific and Technical Information of China (English)

    张小琴; 赵晴; 王帅星; 严小梅; 张传波

    2011-01-01

    通过单因素实验优化镁合金植酸转化工艺,发现在植酸体积浓度为10 mL/L,pH=2.5,温度50℃,成膜时间20 min条件下,可在MB8镁合金表面制备出均匀一致的植酸转化膜.该膜层微观形貌与铬酸盐转化膜类似,表面呈现出均匀分布的网状微裂纹,类似于"龟裂的土地",膜层主要成分为Mg,O,P,Mn和C;动电位极化曲线测试发现MB8镁合金经植酸转化处理后,自腐蚀电流密度显著降低,表明植酸转化膜有效提高了MB8镁舍金的耐蚀性.%The phytic acid conversion technique was investigated by the single-factor experiments. It was found that the uniform coherent conversion coating on the MB8 magnesium alloy can be obtained on the condition of 10 ml/L phytic acid,pH=2.5,at 50 ℃ for 20 min. The micro-structure of the coating was similar with the chromate conversion coating, whose surface was uniform micro-crack network, similar to “the rimose land”. The main elements of the coating were Mg,O,P, Mn and C. The potentiodynamic polarization curves showed that the corrosion current density reduced greatly when the MB8 magnesium alloy was treated through phytic acid conversion technique. Which indicated that the phytic acid conversion coating improved effectively the corrosion resistance of MB8 magnesium alloy.

  10. Androstane derivatives induce apoptotic death in MDA-MB-231 breast cancer cells.

    Science.gov (United States)

    Jakimov, Dimitar S; Kojić, Vesna V; Aleksić, Lidija D; Bogdanović, Gordana M; Ajduković, Jovana J; Djurendić, Evgenija A; Penov Gaši, Katarina M; Sakač, Marija N; Jovanović-Šanta, Suzana S

    2015-11-15

    Biological investigation was conducted to study in vitro antiproliferative and pro-apoptotic potential of selected 17α-picolyl and 17(E)-picolinylidene androstane derivatives. The antiproliferative impact was examined on six human tumor cell lines, including two types of breast (MCF-7 and MDA-MB-231), prostate (PC3), cervical (HeLa), colon (HT 29) and lung cancer (A549), as well as one normal fetal lung fibroblasts cell line (MRC-5). All derivatives selectively decreased proliferation of estrogen receptor negative MDA-MB-231 breast cancer cells after 48 h and 72 h treatment and compounds showed time-dependent activity. We used this cell line to investigate cell cycle modulation and apoptotic cell death induction by flow cytometry, expression of apoptotic proteins by Western blot and apoptotic morphology by visual observation. Tested androstane derivatives affected the cell cycle distribution and induced apoptosis and necrosis. Compounds had different and specific mode of action, depending on derivative type and exposure time. Some compounds induced significant apoptosis measured by Annexin V test compared to reference compound formestane. Higher expression of pro-apoptotic BAX, downregulation of anti-apoptotic Bcl-2 and cleavage of PARP protein were confirmed in almost all treated samples, but the lack of caspase-3 activation suggested the induction of apoptosis in caspase-independent manner. More cells with apoptotic morphology were observed in samples after prolonged treatment. Structure-activity relationship analysis was performed to find correlations between the structure variations of investigated derivatives and observed biological effects. Results of this study showed that some of the investigated androstane derivatives have good biomedical potential and could be candidates for anticancer drug development.

  11. Indoor coverage improvement of MB-OFDM UWB signals with radio over POF system

    Science.gov (United States)

    Lethien, Christophe; Loyez, Christophe; Vilcot, Jean-Pierre; Clavier, Laurent; Bocquet, Michael; Rolland, Paul Alain

    2009-12-01

    A radio over fiber system using the fluorinated based polymer multimode fibers (PMMF) is presented in this paper for the enhancement of the indoor coverage of the multiband orthogonal frequency division multiplexing ultra-wideband standard (MB-OFDM UWB) inside a building. A preliminary part related the cost analysis owing to glass and polymer multimode fiber deployment inside a fiber network is reported. The study of the physical properties of the polymer optical fibers (core diameter, numerical aperture, differential mode delays, modal bandwidth…) is firstly performed in order to effectively exhibit the potentialities and the robustness of such fibers to be used in a low cost radio over fiber system. The DMD measurements of four fluorinated based polymer optical fiber are reported. The designed system operates at 850 nm with commercial off the shelf (COTS) devices combined to the intensity modulation/direct detection technique. The opportunity of using polymer fibers and COTS components to improve the indoor coverage of the MB-OFDM UWB standard is so reported by the measurement of the Error Vector Magnitude or the Relative Constellation Error variation as a function of the system parameters (RF power, optical attenuation, fiber length…) as well as the compliance of the eye diagram with the mask testing. By the way, the transmission performance of both 200 and 480 Mbps signals is demonstrated over up to 200 m link length of polymer multimode fibers: transmission penalties are quantified by relative constellation error with values under the standard requirements. A comparative study with classical OM2 50 μm based glass multimode fiber having the same bandwidth/length product than the PMMF is done.

  12. Grape seed extract suppresses MDA-MB231 breast cancer cell migration and invasion.

    Science.gov (United States)

    Dinicola, Simona; Pasqualato, Alessia; Cucina, Alessandra; Coluccia, Pierpaolo; Ferranti, Francesca; Canipari, Rita; Catizone, Angela; Proietti, Sara; D'Anselmi, Fabrizio; Ricci, Giulia; Palombo, Alessandro; Bizzarri, Mariano

    2014-01-01

    Breast cancer remains a leading cause of mortality among women. In metastasis, cascade migration of cancer cells and invasion of extracellular matrix (ECM) represent critical steps. Urokinase-type plasminogen activator (uPA), as well as metalloproteinases MMP-2 and MMP-9, strongly contribute to ECM remodelling, thus becoming associated with tumour migration and invasion. In addition, the high expression of cytoskeletal (CSK) proteins, as fascin, has been correlated with clinically aggressive metastatic tumours, and CSK proteins are thought to affect the migration of cancer cells. Consumption of fruits and vegetables, characterized by high procyanidin content, has been associated to a reduced mortality for breast cancer. Therefore, we investigated the biological effect of grape seed extract (GSE) on the highly metastatic MDA-MB231 breast cancer cell line, focusing on studying GSE ability in inhibiting two main metastatic processes, i.e., cell migration and invasion. After MDA-MB231 breast cancer cells stimulated with GSE migration and invasion were evaluated by means of trans-well assays and uPA as well as MMPs activity was detected by gelatin zymography. Fascin, β-catenin and nuclear factor-κB (NF-κB) expression were determined using western blot technique. β-Catenin localization was observed by confocal microscopy. We observed that high concentrations of GSE inhibited cell proliferation and apoptosis. Conversely, low GSE concentration decreased cell migration and invasion, likely by hampering β-catenin expression and localization, fascin and NF-κB expression, as well as by decreasing the activity of uPA, MMP-2 and MMP-9. These results make GSE a powerful candidate for developing preventive agents against cancer metastasis.

  13. A selective sweep of >8 Mb on chromosome 26 in the Boxer genome

    Directory of Open Access Journals (Sweden)

    Altet Laura

    2011-07-01

    Full Text Available Abstract Background Modern dog breeds display traits that are either breed-specific or shared by a few breeds as a result of genetic bottlenecks during the breed creation process and artificial selection for breed standards. Selective sweeps in the genome result from strong selection and can be detected as a reduction or elimination of polymorphism in a given region of the genome. Results Extended regions of homozygosity, indicative of selective sweeps, were identified in a genome-wide scan dataset of 25 Boxers from the United Kingdom genotyped at ~20,000 single-nucleotide polymorphisms (SNPs. These regions were further examined in a second dataset of Boxers collected from a different geographical location and genotyped using higher density SNP arrays (~170,000 SNPs. A selective sweep previously associated with canine brachycephaly was detected on chromosome 1. A novel selective sweep of over 8 Mb was observed on chromosome 26 in Boxer and for a shorter region in English and French bulldogs. It was absent in 171 samples from eight other dog breeds and 7 Iberian wolf samples. A region of extended increased heterozygosity on chromosome 9 overlapped with a previously reported copy number variant (CNV which was polymorphic in multiple dog breeds. Conclusion A selective sweep of more than 8 Mb on chromosome 26 was identified in the Boxer genome. This sweep is likely caused by strong artificial selection for a trait of interest and could have inadvertently led to undesired health implications for this breed. Furthermore, we provide supporting evidence for two previously described regions: a selective sweep on chromosome 1 associated with canine brachycephaly and a CNV on chromosome 9 polymorphic in multiple dog breeds.

  14. Graded hypoxia modulates the invasive potential of HT1080 fibrosarcoma and MDA MB231 carcinoma cells.

    Science.gov (United States)

    Subarsky, Patrick; Hill, Richard P

    2008-01-01

    Spatial and temporal oxygen heterogeneity exists in most solid tumour microenvironments due to an inadequate vascular network supplying a dense population of tumour cells. An imbalance between oxygen supply and demand leads to hypoxia within a significant proportion of a tumour, which has been correlated to the likelihood of metastatic dissemination in both rodent tumour models and human patients. Experimentally, it has been demonstrated that near-anoxic in vitro exposure results in transiently increased metastatic potential in some tumour cell lines. The purpose of this study was to examine the effect of graded low oxygen conditions on the invasive phenotype of human tumour cells using an in vitro model of basement membrane invasion, in which we measured oxygen availability directly at the invasion surface of the transwell chamber. Our results show a relationship between culture vessel geometry and time to achieve hypoxia which may affect the interpretation of low oxygen experiments. We exposed the human tumour cell lines, HT1080 and MDA MB231, to graded normobaric oxygen (5% O(2)-0.2% O(2)) either during or prior to in vitro basement membrane invasion to simulate conditions of intravasation and extravasation. A secondary aim was to investigate the potential regulation of matrix metalloproteinase activity by oxygen availability. We identified significant reductions in invasive ability under low oxygen conditions for the HT1080 cell line and an increase in invasion at intermediate oxygen conditions for the MDA MB231 cell line. There were differences in the absolute activity of the individual matrix metalloproteinases, MMP-2, -9, -14, between the two cell lines, however there were no significant changes following exposure to hypoxic conditions. This study demonstrates cell line specific effects of graded oxygen levels on invasive potential and suggests that intermediate levels of low oxygen may increase metastatic dissemination.

  15. Application of MB+ network technology on automatic control system of power plant%MB+网络技术在电厂自动控制系统中的应用

    Institute of Scientific and Technical Information of China (English)

    高素萍

    2006-01-01

    基于MB+网络技术和PLC控制技术,结合垃圾焚烧发电厂工艺流程特点,论述了MB+网络技术在垃圾焚烧发电厂自动控制系统中的应用,并对垃圾焚烧装置和垃圾燃烧过程的自动控制及系统的监控功能进行了阐述.

  16. 基于MB 90543的高性能GPS同步时钟研制%Development of high performance GPS synchronizing clock based on MB 90543

    Institute of Scientific and Technical Information of China (English)

    邹晖; 李志康; 黄益庄; 庄清泉

    2006-01-01

    全球定位系统(GPS)同步时钟由GPS接收器、单片机及其外围电路组成.以MB 90543为核心的时钟中心处理单元负责读取GPS接收器发送的数据信息并作适当处理,从中获取ASCⅡ码国际标准时间的时间信息,经转换为BCD码后的串行时间信息供显示.该时钟选用可供嵌入式系统应用的M12 GPS接收器,可输出时间精度为1μs的秒脉冲.测频和数码管显示电路保证了电网频率的实时测量和显示.时钟软件包括初始化、接收器收/发、串口/CAN总线收/发、显示等子程序.采用串行通信方式授时,串行数据的收/发均采用中断方式,能提供日期和时间信息;而采用脉冲校时,可保证装置的校时误差小于50μs.在时钟装置中,采用以上2种综合校时方式.指出正确识别秒脉冲信号是确保同步校时的关键,软件及外部硬件计数阵列给秒脉冲信号加硬件窗的抗干扰措施效果良好.所设计的同步时钟投入运行后表明,符合设计要求.

  17. Transcriptional program induced by factor VIIa-tissue factor, PAR1 and PAR2 in MDA-MB-231 cells

    DEFF Research Database (Denmark)

    Albrektsen, Tatjana; Sørensen, B B; Hjortø, G M

    2007-01-01

    -activated receptor 1 (PAR1) or PAR2 agonists using MDA-MB-231 breast carcinoma cells that constitutively express TF, PAR1 and PAR2. RESULTS AND CONCLUSIONS: Out of 8500 genes, FVIIa stimulation induced differential regulation of 39 genes most of which were not previously recognized as FVIIa regulated. All genes...... regulated genes encode cytokines, chemokines and growth factors, and the gene repertoire induced by FVIIa in MDA-MB-231 cells is consistent with a role for TF-FVIIa signaling in regulation of a wound healing type of response. Interestingly, a number of genes regulated exclusively by FVIIa/PAR2-mediated cell...... signaling in MDA-MB-231 cells were regulated by thrombin and a PAR1 agonist, but not by FVIIa, in the TF-expressing glioblastoma U373 cell line....

  18. Polyphenols from Artemisia annua L Inhibit Adhesion and EMT of Highly Metastatic Breast Cancer Cells MDA-MB-231.

    Science.gov (United States)

    Ko, Young Shin; Lee, Won Sup; Panchanathan, Radha; Joo, Young Nak; Choi, Yung Hyun; Kim, Gon Sup; Jung, Jin-Myung; Ryu, Chung Ho; Shin, Sung Chul; Kim, Hye Jung

    2016-07-01

    Recent evidence suggests that polyphenolic compounds from plants have anti-invasion and anti-metastasis capabilities. The Korean annual weed, Artemisia annua L., has been used as a folk medicine for treatment of various diseases. Here, we isolated and characterized polyphenols from Korean A. annua L (pKAL). We investigated anti-metastatic effects of pKAL on the highly metastatic MDA-MB-231 breast cancer cells especially focusing on cancer cell adhesion to the endothelial cell and epithelial-mesenchymal transition (EMT). Firstly, pKAL inhibited cell viability of MDA-MB-231 cells in a dose-dependent manner, but not that of human umbilical vein endothelial cells (ECs). Polyphenols from Korean A. annua L inhibited the adhesion of MDA-MB-231 cells to ECs through reducing vascular cell adhesion molecule-1 expression of MDA-MB-231 and ECs, but not intracellular adhesion molecule-1 at the concentrations where pKAL did not influence the cell viability of either MDA-MB-231 cells nor EC. Further, pKAL inhibited tumor necrosis factor-activated MDA-MB-231 breast cancer cell invasion through inhibition of matrix metalloproteinase-2 and matrix metalloproteinase-9 and EMT. Moreover, pKAL inhibited phosphorylation of Akt, but not that of protein kinase C. These results suggest that pKAL may serve as a therapeutic agent against cancer metastasis at least in part by inhibiting the cancer cell adhesion to ECs through suppression of vascular cell adhesion molecule-1 and invasion through suppression of EMT. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

  19. Significant Association of Serum Adiponectin and Creatine Kinase-MB Levels in ST-Segment Elevation Myocardial Infarction.

    Science.gov (United States)

    Natsukawa, Tomoaki; Maeda, Norikazu; Fukuda, Shiro; Yamaoka, Masaya; Fujishima, Yuya; Nagao, Hirofumi; Sato, Fumi; Nishizawa, Hitoshi; Sawano, Hirotaka; Hayashi, Yasuyuki; Funahashi, Tohru; Kai, Tatsuro; Shimomura, Iichiro

    2017-08-01

    Adiponectin, an adipocyte-specific secretory protein, abundantly exists in the blood stream while its concentration paradoxically decreases in obesity. Hypoadiponectinemia is one of risks of cardiovascular diseases. However, impact of serum adiponectin concentration on acute ischemic myocardial damages has not been fully clarified. The present study investigated the association of serum adiponectin and creatine kinase (CK)-MB levels in subjects with ST-segment elevation myocardial infarction (STEMI). This study is a physician-initiated observational study and is also registered with the University Hospital Medical Information Network (Number: UMIN 000014418). Patients were admitted to Senri Critical Care Medical Center, given a diagnosis of STEMI, and treated by primary percutaneous coronary intervention (PCI). Finally, 49 patients were enrolled and the association of serum adiponectin, CK-MB, and clinical features were mainly analyzed. Serum adiponectin levels decreased rapidly and reached the bottom at 24 hours after recanalization. Such reduction of serum adiponectin was inversely correlated with the area under the curve (AUC) of serum CK-MB (p=0.013). Serum adiponectin concentrations were inversely correlated with AUC of serum CK-MB. In multivariate analysis, serum adiponectin concentration on admission (p=0.002) and collateral (p=0.037) were significantly and independently correlated with serum AUC of CK-MB. Serum AUC of CK-MB in STEMI subjects was significantly associated with serum adiponectin concentration on admission and reduction of serum adiponectin levels from baseline to bottom. The present study may provide a possibility that serum adiponectin levels at acute phase are useful in the prediction for prognosis after PCI-treated STEMI subjects.

  20. Effect of Polygonatum odoratum extract on human breast cancer MDA-MB-231 cell proliferation and apoptosis.

    Science.gov (United States)

    Tai, Yu; Sun, Yi-Ming; Zou, Xue; Pan, Qiong; Lan, Ya-Dong; Huo, Qiang; Zhu, Jing-Wen; Guo, Fei; Zheng, Chang-Quan; Wu, Cheng-Zhu; Liu, Hao

    2016-10-01

    Traditional Chinese medicine (TCM) is important in the provision of anti-tumor drugs. Recently, studies have shown that certain types of TCM agents are able to control the growth of tumors, enhance the body's immune function and enhance the therapeutic effect of chemotherapeutic drugs. In women, breast carcinoma is the most common tumor type and the second most common cause of death from cancer. Polygonatum odoratum (P. odoratum) is commonly used in TCM. The aim of the present study was to investigate the effects of P. odoratum extract on the proliferation and apoptosis of MDA-MB-231 breast cancer cells. Cell proliferation was assessed using MTT and colony formation assays. In addition, propidium iodide (PI)/Annexin V-FITC staining was used to investigate the apoptosis of MDA-MB-231 cells following treatment with P. odoratum extract. The protein expression levels of B-cell lymphoma-2 (Bcl-2) and Bcl-2-associated X protein (Bax) were also detected using western blot analysis, while a JC-1 staining assay was used to assess the mitochondrial membrane potential (ΔΨm). The results of the MTT assay showed that the proliferation and colony formation of MDA-MB-231 cells were inhibited following treatment with the extract. Furthermore, the PI/Annexin-V staining showed that the apoptosis of MDA-MB-231 cells was enhanced by the extract, in a concentration-dependent manner. The extract also lowered the ΔΨm of MDA-MB-231 cells, upregulated the expression of Bax and inhibited the expression of Bcl-2. In conclusion, these results showed that the P. odoratum extract inhibited the proliferation and induced apoptosis of breast cancer MDA-MB-231 cells.

  1. Distribution & diagnostic efficacy of cardiac markers CK-MB & LDH in pericardial fluid for postmortem diagnosis of ischemic heart disease.

    Science.gov (United States)

    Ghormade, Pankaj Suresh; Kumar, Narendra Baluram; Tingne, Chaitanya Vidyadhar; Keoliya, Ajay Narmadaprasad

    2014-11-01

    The aim of the present study is to evaluate the diagnostic efficacy of biochemical markers creatine kinase-MB (CK-MB) and LDH in pericardial fluid for postmortem diagnosis of ischemic heart disease (IHD). We studied 119 medico-legal autopsies selected during a period of 2 years. Subjects were assigned into diagnostic groups upon final cause of death as follows: (1) sudden cardiac death due to IHD's (n = 52), (2) violent asphyxia (n = 24); (3) polytraumatic deaths (n = 20); (4) natural deaths excluding cardiac causes (n = 23). Pericardial fluid samples were tested for estimating enzyme levels. Histological examination was performed with hematoxylin and eosin (H&E) stain on myocardial tissue samples. We observed highest levels of CK-MB & LDH in deaths due to IHD's. Kruskal-Wallis test revels significant differences in activities of CK-MB (P = 0.0001) and LDH (P = 0.0065) amongst all diagnostic groups. Mann-Whitney test showed highly significant (P MB in group 1 as compared to other diagnostic groups. However, LDH levels were non-discriminatory (P = 0.0827) between cases of IHD's and cases of other natural deaths. CK-MB levels were statistically non-significant between cases divided as myocardial infarction (MI) and severe coronary artery disease in group 1, hence its role for postmortem detection of MI is somewhat limiting. However, sensitivity and negative predictive values of its cut off level obtained in cases of IHD's are nearly equal to diagnostic efficacy in clinical settings. Hence, it can be useful additional diagnostic tool for autopsy diagnosis of IHD's. Whereas, LDH is not useful for postmortem diagnosis in these cases. Copyright © 2014 Elsevier Ltd and Faculty of Forensic and Legal Medicine. All rights reserved.

  2. Intrinsic Resistance to 5-Fluorouracil in a Brain Metastatic Variant of Human Breast Cancer Cell Line, MDA-MB-231BR.

    Science.gov (United States)

    Sagara, Atsunobu; Igarashi, Katsuhide; Otsuka, Maky; Karasawa, Takeshi; Gotoh, Noriko; Narita, Michiko; Kuzumaki, Naoko; Narita, Minoru; Kato, Yoshinori

    2016-01-01

    Although drug resistance is often observed in metastatic recurrence of breast cancer, little is known about the intrinsic drug resistance in such metastases. In the present study, we found, for the first time, that MDA-MB-231BR, a brain metastatic variant of a human breast cancer cell line, was refractory to treatment with 5-fluorouracil (5-FU) even without chronic drug exposure, compared to its parent cell line, MDA-MB-231, and a bone metastatic variant, MDA-MB-231SCP2. Both the mRNA and protein levels of COX-2 and BCL2A1 in MDA-MB-231BR were significantly higher than those in MDA-MB-231 or MDA-MB-231SCP2. Neither the COX-2 inhibitor celecoxib nor the NF-κB inhibitor BAY11-7082 could sensitize MDA-MB-231BR to 5-FU, indicating that COX-2 plays little, if any, role in the resistance of MDA-MB-231BR to 5-FU. Although BCL2-family inhibitor ABT-263 failed to sensitize MDA-MB-231BR to 5-FU at a dose at which ABT-263 is considered to bind to BCL2, BCL2-xL, and BCL2-w, but not to BCL2A1, ABT-263 did sensitize MDA-MB-231BR to 5-FU to a level comparable to that in MDA-MB-231 at a dose of 5 μM, at which ABT-263 may disrupt intracellular BCL2A1 protein interactions. More importantly, BCL2A1 siRNA sensitized MDA-MB-231BR to 5-FU, whereas the overexpression of BCL2A1 conferred 5-FU-resistance on MDA-MB-231. These results indicate that BCL2A1 is a key contributor to the intrinsic 5-FU-resistance in MDA-MB-231BR. It is interesting to note that the drug sensitivity of MDA-MB-231BR was distinct from that of MDA-MB-231SCP2 even though they have the same origin (MDA-MB-231). Further investigations pertinent to the present findings may provide valuable insight into the breast cancer brain metastasis.

  3. Transcriptional program induced by factor VIIa-tissue factor, PAR1 and PAR2 in MDA-MB-231 cells

    DEFF Research Database (Denmark)

    Albrektsen, T; Sørensen, B B; Hjortø, G M

    2007-01-01

    -regulated genes was also regulated by a PAR1 agonist peptide suggesting extensive redundancy between FVIIa/PAR2 signaling and thrombin/PAR1 signaling. The FVIIa regulated genes encode cytokines, chemokines and growth factors, and the gene repertoire induced by FVIIa in MDA-MB-231 cells is consistent...... with a role for TF-FVIIa signaling in regulation of a wound healing type of response. Interestingly, a number of genes regulated exclusively by FVIIa/PAR2-mediated cell signaling in MDA-MB-231 cells were regulated by thrombin and a PAR1 agonist, but not by FVIIa, in the TF-expressing glioblastoma U373 cell...

  4. EFFECT OF GENISTEIN ON EXPRESSION OF VASCULAR ENDOTHELIAL GROWTH FACTOR IN MDA-MB-453 BREAST CANCER CELLS%染料木黄酮对MDA-MB-453乳腺癌细胞VEGF表达的影响

    Institute of Scientific and Technical Information of China (English)

    余小平; 糜漫天; 朱俊东

    2005-01-01

    目的:观察染料木黄酮(genistein,Gen)对MDA-MB-453乳腺癌细胞血管内皮生长因子(vascular endothelial growth factor,VEGF)表达的影响,探讨Gen抗HER-2/neu高表达乳腺癌血管生成的分子机制.方法:5×10-5mol/L Gen处理MDA-MB-453细胞24、48、72 h后,应用免疫组织化学、Western blot及RT-PCR法检测MDA-MB-453乳腺癌细胞VEGF的表达变化.结果:Gen处理MDA-MB-453细胞24、48、72 h后,VEGF的mRNA和蛋白表达量随着处理时间的延长逐渐下降.结论:Gen能在转录和翻译水平下调HER-2/neu高表达乳腺癌细胞VEGF的表达,这可能是Gen抑制HER-2/neu高表达乳腺癌血管生成的机制之一.

  5. ADM与5Fu可抑制MDA-MB231乳癌细胞的SNCG表达%ADM and 5Fu inhibit the synuclein-γexpression of MDA-MB231 breast cancer cells

    Institute of Scientific and Technical Information of China (English)

    袁光波; 张幸平; 何金花; 唐卫军; 陈睿

    2008-01-01

    目的 研究乳癌临床常用化疗药物顺铂(ciaplalin or DDP),阿霉素(adriamycin,ADM),氟尿嘧啶(fluorouradl,5Fu)对MDA-MB231乳癌细胞SNCG表达的干扰效应.方法 通过RT-PCR及免疫组织化学法检测上述药物处理组和阴性对照组MDA-MB231细胞的SNCG表达状况,用Quantity One软件及北航真彩色医学图像处理系统(CM-20008)分别对各组SNCG mR-NA和蛋白相对表达水平进行分析.结果 ADM和5Fu处理组与阴性对照组比较,MDA-MB231细胞的SNCG mRNA及蛋白表达水平差异均有统计学意义(P值均小于0.05),而DDP处理组表达水平与对照组无差别.结论 ADM和5Fu可抑制MDA-MB231细胞的SNCG表达.

  6. Radiation Evaluation of an Advanced 64Mb 3.3V DRAM and Insights into the Effects of Scaling on Radiation Hardness

    Science.gov (United States)

    Shaw, D. C.; Swift, G. M.; Johnston, A. H.

    1995-01-01

    In this paper, total ionizing dose radiation evaluations of the Micron 64 Mb 3.3 V, fast page mode DRAM and the IBM LUNA-ES 16 Mb DRAM are presented. The effects of scaling on total ionizing dose radiation hardness are studied utilizing test structures and a series of 16 Mb DRAMs with different feature sizes from the same manufacturing line. General agreement was found between the threshold voltage shifts of 16 Mb DRAM test structures and the threshold voltage measured on complete circuits using retention time measurements. Retention time measurement data from early radiation doses are shown that allow internal failure modes to be distinguished.

  7. Anticancer property of sediment actinomycetes against MCF-7 and MDA-MB-231 cell lines

    Institute of Scientific and Technical Information of China (English)

    Ravikumar S; Fredimoses M; Gnanadesigan M

    2012-01-01

    Objective: To investigate the anticancer property of marine sediment actinomycetes against two different breast cancer cell lines. Methods:In vitro anticancer activity was carried out against breast (MCF-7 and MDA-MB-231) cancer cell lines. Partial sequences of the 16s rRNA gene, phylogenetic tree construction, multiple sequence analysis and secondary structure analysis were also carried out with the actinomycetes isolates. Results: Of the selected five actinomycete isolates, ACT01 and ACT02 showed the IC50 value with (10.13±0.92) and (22.34±5.82)μg/mL concentrations, respectively for MCF-7 cell line at 48 h, but ACT01 showed the minimum (18.54±2.49 μg/mL) level of IC50 value with MDA-MB-231 cell line. Further, the 16s rRNA partial sequences of ACT01, ACT02, ACT03, ACT04 and ACT05 isolates were also deposited in NCBI data bank with the accession numbers of GQ478246, GQ478247, GQ478248, GQ478249 and GQ478250, respectively. The phylogenetic tree analysis showed that, the isolates of ACT02 and ACT03 were represented in group I and III, respectively, but ACT01 and ACT02 were represented in group II. The multiple sequence alignment of the actinomycete isolates showed that, the maximum identical conserved regions were identified with the nucleotide regions of 125 to 221st base pairs, 65 to 119th base pairs and 55, 48 and 31st base pairs. Secondary structure prediction of the 16s rRNA showed that, the maximum free energy was consumed with ACT03 isolate (-45.4 kkal/mol) and the minimum free energy was consumed with ACT04 isolate (-57.6 kkal/mol). Conclusions:The actinomycete isolates of ACT01 and ACT02 (GQ478246 and GQ478247) which are isolated from sediment sample can be further used as anticancer agents against breast cancer cell lines.

  8. In vitro cultivation of malignant lymphoblasts of transplantable Mouse Lymphosarcoma MB (T 86157) without typical mesenchyme cells

    NARCIS (Netherlands)

    Bruyn, de Willemina M.

    1949-01-01

    Previous investigations (see Literature) by means of tissue culture methods have shown that the malignant lymphoblasts of mouse lymphosarcoma MB (T 86157) can be cultivated indefinitely when in the presence of actively growing mesenchyme cells. Under the cultural conditions provided, which included

  9. Whole-Genome Sequences of Mycobacterium bovis Strain MbURU-001, Isolated from Fresh Bovine Infected Samples

    Science.gov (United States)

    Lasserre, Moira; Berná, Luisa; Greif, Gonzalo; Díaz-Viraqué, Florencia; Naya, Hugo; Castro-Ramos, Miguel; Juambeltz, Arturo

    2015-01-01

    Bovine tuberculosis in cattle has a high incidence in Uruguay, where it is considered a disease of national importance. We present the genome sequence of Mycobacterium bovis strain MbURU-001, isolated from pectoral lymph nodes of a bovine host from a cattle farm. PMID:26543108

  10. [N-acetyl-cystein-(NAC)-activated creatinkinase (CK) and isoenzyme CK-MB in the serum of children].

    Science.gov (United States)

    Sitzmann, F C; Orth, H

    1983-08-01

    We have examined the variation of creatinekinase levels (NAC-activated) with age in 170 children. The subjects included 40 neonates, 18 premature neonates, 40 small babies, 32 infants and 40 schoolchildren. The enzyme activity of CK-MM was very high in the first hours after delivery and remained high for a few days. The isoenzyme MB in healthy newborns also showed a higher catalytic concentration. These values (about 2-12 U/l) reached normal levels of adults within 4 months of life (0.5-5 U/l). The same rule applied to CK-MM: enzyme activities of 160 U/l and more in the first days of life declined to 16-75 U/l during the first 4 months. No correlation between birth trauma and the increase in serum-CK was found. Because of the increased CK-MM (and CK-MB) found in normal newborns screening for Duchenne-type muscular dystrophy should be postponed for a few weeks after delivery. In view of the relatively high endogenous serum CK-MB in the neonates (release of CK-MB from the skeletal muscle) the test lacks the specificity for cardiac damage. Intramuscular injections of several drugs lead to a distinct increase in CK activity. A rise of CK-MM was seen 4-24 h after catheterization of the heart.

  11. The influence of hemolysis, turbidity and icterus on the measurements of CK-MB, troponin I and myoglobin.

    Science.gov (United States)

    Kwon, Hi J; Seo, Eun J; Min, Ki O

    2003-03-01

    To decide on the acceptability of a specimen for the measurement of serum CK-MB, troponin I and myoglobin, we investigated the influence of hemolysis, turbidity, and icterus on those tests by adding arbitrarily made interferents. A total of 16 cases each for CK-MB and troponin I, and 18 cases for myoglobin tests were studied to verify the effects of hemolysis, turbidity, and unconjugated hyperbilirubinemia. A total of 16 cases were studied to clarify the effects of the conjugated hyperbilirubinemia. We graded the severity of hemolysis, turbidity, and icterus as mild, moderate, and severe after adding hemolysate, Intralipos (20% soybean oil), and unconjugated or conjugated bilirubin to sera. ACS180SE automated chemiluminescence system was used to measure CK-MB, troponin I, and myoglobin. CK-MB and troponin I were affected by any degree of hemolysis, turbidity, unconjugated hyperbilirubinemia, and conjugated hyperbilirubinemia, while myoglobin was affected only by severe unconjugated hyperbilirubinemia and conjugated hyperbilirubinemia in the samples with concentration higher than reference range, resulting in concentration level lower than baseline. In conclusion, the results of cardiac markers should be carefully interpreted when the specimens are hemolyzed, turbid or icteric.

  12. Prognostic value of MRD-dynamics in childhood acute lymphoblastic leukemia treated according to the MB-2002/2008 protocols.

    Science.gov (United States)

    Meleshko, Alexander N; Savva, Natalia N; Fedasenka, Uladzimir U; Romancova, Alexandra S; Krasko, Olga V; Eckert, Cornelia; von Stackelberg, Arend; Aleinikova, Olga V

    2011-10-01

    Detection of minimal residual disease (MRD) during the treatment of acute lymphoblastic leukemia (ALL) by RQ-PCR analysis of clonal Ig/TCR rearrangements is used for risk group stratification in European treatment protocols. In Belarus patients with childhood ALL are treated according to ALL-MB protocols, which do not use MRD-based risk stratification. To evaluate the prognostic significance of MRD for ALL-MB-2002/2008 protocols, MRD was quantified by RQ-PCR in 68 ALL patients at four time points: on day 15, on day 36, before and after maintenance therapy (MT). MRD positivity, as well as quantitative level of MRD were analyzed and compared between patients who stayed in remission and relapsed. Relapse-free survival revealed to be significantly associated with MRD levels at different time points. Unfavorable prognosis was shown for MRD≥10(-3) on day 36 (pPCR in children with ALL treated with ALL-MB protocols is feasible and independently associated with outcome. MRD may be a suitable parameter for treatment stratification in MB protocols in future.

  13. Cardiac troponin T and CK-MB mass release after visually successful percutaneous transluminal coronary angioplasty in stable angina pectoris

    DEFF Research Database (Denmark)

    Ravkilde, J; Nissen, H; Mickley, H

    1994-01-01

    The incidence of cardiac troponin T (Tn-T) and creatine kinase (CK) isoenzyme MB mass release was studied in 23 patients with stable angina pectoris undergoing visually successful percutaneous transluminal coronary angioplasty (PTCA). Serial blood samples were drawn for measurement of serum Tn...

  14. Monitoring of tumor growth and metastasis potential in MDA-MB-435s/ tk-luc human breast cancer xenografts

    Science.gov (United States)

    Chang, Ya-Fang; Lin, Yi-Yu; Wang, Hsin-Ell; Liu, Ren-Shen; Pang, Fei; Hwang, Jeng-Jong

    2007-02-01

    Molecular imaging of reporter gene expression provides a rapid, sensitive and non-invasive monitoring of tumor behaviors. In this study, we reported the establishment of a novel animal model for longitudinal examination of tumor growth kinetics and metastatic spreading in vivo. The highly metastatic human breast carcinoma MDA-MB-435s cell line was engineered to stably express herpes simplex virus type 1 thymidine kinase (HSV-1- tk) and luciferase ( luc). Both 131I-FIAU and D-luciferin were used as reporter probes. For orthotopic tumor formation, MDA-MB-435s/ tk-luc cells were implanted into the first nipple of 6-week-old female NOD/SCID mice. For metastatic study, cells were injected via the lateral tail vein. Mice-bearing MDA-MB-435s/ tk-luc tumors were scanned for tumor growth and metastatsis using Xenogen IVIS50 system. Gamma scintigraphy and whole-body autoradiography were also applied to confirm the tumor localization. The results of bioluminescence imaging as well as histopathological finding showed that tumors could be detected in femur, spine, ovary, lungs, kidney, adrenal gland, lymph nodes and muscle at 16 weeks post i.v. injection, and correlated photons could be quantified. This MDA-MB-435s/ tk-luc human breast carcinoma-bearing mouse model combined with multimodalities of molecular imaging may facilitate studies on the molecular mechanisms of cancer invasion and metastasis.

  15. Comparison of ASAT, CK, CK-MB, and LD for the estimation of acute myocardial infarct size in man.

    Science.gov (United States)

    Grande, P; Christiansen, C; Alstrup, K

    1983-03-14

    The purpose of this study was to set up a simple and reliable procedure for estimating acute myocardial infarct (AMI) size by measuring serum enzymes in a few daily blood samples. Peak enzyme values and estimated infarct size from one, two, or three daily samples of aspartate aminotransferase (ASAT), creatine kinase (CK), CK-MB, and lactate dehydrogenase (LD) were compared with the extent of myocardial necrosis measured at autopsy in 22 patients who died from AMI. The correlation between the extent of the necrosis measured and peak serum enzymes from one daily blood sample was highest for CK-MB (r = 0.78) and LD (r = 0.73) compared to CK (r = 0.68) and ASAT (r = 0.67). To obtain a significant correlation, however, two patients had to be excluded from the ASAT and LD analyses. No significant improvement was obtained by more frequent blood sampling. Estimation of infarct size did not improve the correlation significantly for any enzyme, although the coefficient of correlation for CK-MB increased slightly (r = 0.83). Serum CK-MB determination provides a semiquantitative estimate of infarct size, but the other enzymes may give erroneous estimates owing to lesser cardiospecificity.

  16. Production and characterisation of glycolipid biosurfactant by Halomonas sp. MB-30 for potential application in enhanced oil recovery.

    Science.gov (United States)

    Dhasayan, Asha; Kiran, G Seghal; Selvin, Joseph

    2014-12-01

    Biosurfactant-producing Halomonas sp. MB-30 was isolated from a marine sponge Callyspongia diffusa, and its potency in crude oil recovery from sand pack column was investigated. The biosurfactant produced by the strain MB-30 reduced the surface tension to 30 mN m(-1) in both glucose and hydrocarbon-supplemented minimal media. The critical micelle concentration of biosurfactant obtained from glucose-based medium was at 0.25 mg ml(-1) at critical micelle dilution 1:10. The chemical structure of glycolipid biosurfactant was characterised by infrared spectroscopy and proton magnetic resonance spectroscopy. The emulsification activity of MB-30 biosurfactant was tested with different hydrocarbons, and 93.1 % emulsification activity was exhibited with crude oil followed by kerosene (86.6 %). The formed emulsion was stable for up to 1 month. To identify the effectiveness of biosurfactant for enhanced oil recovery in extreme environments, the interactive effect of pH, temperature and salinity on emulsion stability with crude oil and kerosene was evaluated. The stable emulsion was formed at and above pH 7, temperature >80 °C and NaCl concentration up to 10 % in response surface central composite orthogonal design model. The partially purified biosurfactant recovered 62 % of residual crude oil from sand pack column. Thus, the stable emulsifying biosurfactant produced by Halomonas sp. MB-30 could be used for in situ biosurfactant-mediated enhanced oil recovery process and hydrocarbon bioremediation in extreme environments.

  17. Monitoring of tumor growth and metastasis potential in MDA-MB-435s/tk-luc human breast cancer xenografts

    Energy Technology Data Exchange (ETDEWEB)

    Chang, Y.-F. [Department of Radiological Sciences, National Yang-Ming University, 155, Sec. 2, Li-Nong Street, Pei-tou 112, Taipei, Taiwan (China); Lin, Y.-Y. [Department of Radiological Sciences, National Yang-Ming University, 155, Sec. 2, Li-Nong Street, Pei-tou 112, Taipei, Taiwan (China); Wang, H.-E. [Department of Radiological Sciences, National Yang-Ming University, 155, Sec. 2, Li-Nong Street, Pei-tou 112, Taipei, Taiwan (China); Liu, R.-S. [Department of Nuclear Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan (China); Nuclear Medicine Department, Veterans General Hospital, Taipei, Taiwan (China); Pang Fei [Department of Veterinary Medicine, National Taiwan University, Taipei, Taiwan (China); Hwang, J.-J. [Department of Radiological Sciences, National Yang-Ming University, 155, Sec. 2, Li-Nong Street, Pei-tou 112, Taipei, Taiwan (China)]. E-mail: jjhwang@ym.edu.tw

    2007-02-01

    Molecular imaging of reporter gene expression provides a rapid, sensitive and non-invasive monitoring of tumor behaviors. In this study, we reported the establishment of a novel animal model for longitudinal examination of tumor growth kinetics and metastatic spreading in vivo. The highly metastatic human breast carcinoma MDA-MB-435s cell line was engineered to stably express herpes simplex virus type 1 thymidine kinase (HSV-1-tk) and luciferase (luc). Both {sup 131}I-FIAU and D-luciferin were used as reporter probes. For orthotopic tumor formation, MDA-MB-435s/tk-luc cells were implanted into the first nipple of 6-week-old female NOD/SCID mice. For metastatic study, cells were injected via the lateral tail vein. Mice-bearing MDA-MB-435s/tk-luc tumors were scanned for tumor growth and metastatsis using Xenogen IVIS50 system. Gamma scintigraphy and whole-body autoradiography were also applied to confirm the tumor localization. The results of bioluminescence imaging as well as histopathological finding showed that tumors could be detected in femur, spine, ovary, lungs, kidney, adrenal gland, lymph nodes and muscle at 16 weeks post i.v. injection, and correlated photons could be quantified. This MDA-MB-435s/tk-luc human breast carcinoma-bearing mouse model combined with multimodalities of molecular imaging may facilitate studies on the molecular mechanisms of cancer invasion and metastasis.

  18. MAIZEGDB.ORG, the Maize Genetics Cooperation and the 2500 MB B73 Genome-Generated Tsunami

    Science.gov (United States)

    Advances in sequencing technology have made it possible to sequence the 2500 MB B73 maize genome, both cheaply and in a relatively short time. Nearly simultaneously, other sequencing-based data are on the leading edge of a data tsunami: sequenced differences (currently >300,000 SNP for >1000 inbre...

  19. Interfering with CXCR4 expression inhibits proliferation, adhesion and migration of breast cancer MDA-MB-231 cells.

    Science.gov (United States)

    Guo, Shanyu; Xiao, Dan; Liu, Huihui; Zheng, Xiao; Liu, Lei; Liu, Shougui

    2014-10-01

    To investigate the effect and mechanism of the CXC chemokine receptor 4 (CXCR4) in the proliferation and migration of breast cancer, a short-hairpin RNA (shRNA) eukaryotic expression vector targeting CXCR4 was constructed, and the impact of such on the proliferation, adhesion and migration of human breast cancer MDA-MB-231 cells was observed. The fragments of CXCR4-shRNA were synthesized and cloned into a pGCsi-U6-Neo-green fluorescent protein vector. The recombinant plasmids were transfected into 293T cells and the most efficacious interfering vector was selected. MDA-MB-231 cells were transfected by liposome assay. The effects of silencing CXCR4 expression by shRNA on the growth, adhesion and migration of MDA-MB-231 cells were determined by Cell Counting Kit-8, cell-matrix adhesion and wound-healing assays. The shRNA eukaryotic expression vectors targeting CXCR4 (CXCR4-shRNA) were successfully constructed and transfected into 293T cells. Quantitative polymerase chain reaction and western blot analysis revealed that the maximum inhibitory rate of CXCR4 expression was 81.3%. CXCR4-shRNA transfection significantly inhibited the proliferation of MDA-MB-231 cells (PMB-231 cells and the extracellular matrix (PMB-231 cells in the CXCR4-shRNA transfection group was significantly smaller than that in the control plasmid and blank control groups (PMB-231 cells.

  20. Contribution of various lipid profile parameters in determining creatine kinase-MB levels in unstable angina patients.

    Science.gov (United States)

    Bagale, Kiran R; Ingle, Avinash S; Choudhary, Rajeev

    2016-01-01

    In India, the correlation of severity of minor myocardial damage with dyslipidemia has rarely been studied in patients of unstable angina (UA). Dyslipidemia is proven to be a major risk factor for developing acute coronary syndrome (ACS) but still there is doubt about the type of lipoproteins involved in causing minor myocardial damage occurring in UA patients of ACS. The aim of our study was to find out the contribution of various types of lipoproteins to predict the severity of minor myocardial damage occurring in the patients of UA. Correlation design was used for the study. A single group of individuals was selected. Data were collected on dependent variable creatine kinase-MB (CK-MB) and independent variables (lipid profile parameters). The study comprised fifty patients admitted in cardiac care unit with typical history of UA with electrocardiogram showing no ST-segment elevation. The severity of myocardial damage was assessed from on admission CK-MB levels. The lipid profile was estimated from fasting blood samples of all the patients. For the purpose of the study, Pearson correlation and multiple linear regression analysis methods were applied. The triacylglycerol (TAG), very-low-density lipoprotein (VLDL), total cholesterol/high-density lipoprotein (TC/HDL) showed significant positive correlation whereas HDL was negatively correlating with CK-MB levels. The TAG, VLDL, and TC/HDL were found to be significantly affecting the severity of myocardial damage in the patients of UA.

  1. Potential suppressive effects of gentian violet on human breast cancer MDA-MB-231 cells in vitro: Comparison with gemcitabine.

    Science.gov (United States)

    Yamaguchi, Masayoshi; Murata, Tomiyasu

    2016-08-01

    Gentian violet (GV), a cationic triphenylmethane dye, is used as an antifungal and antibacterial agent. Recently, attention has been focused on GV as a potential chemotherapeutic and antiangiogenic agent. The present study was undertaken to determine the suppressive effects of GV on human breast cancer MDA-MB-231 cells in vitro. The proliferation of MDA-MB-231 cells was suppressed by culture with GV (1-200 nM). The suppressive effects of GV on cell proliferation were not potentiated in the presence of various inhibitors that induce cell cycle arrest in vitro. This finding suggested that GV inhibits G1 and G2/M phase cell cycle arrest in MDA-MB-231 cells. The suppressive effects of GV on proliferation are mediated through the inhibition of various signaling pathways or nuclear transcription in vitro. Moreover, the suppressive effects of GV on cell proliferation were compared with that of gemcitabine, a strong antitumor agent that induces nuclear DNA damage. Notably, the culture with gemcitabine >50 nM suppressed cell proliferation, while the effects of GV were observed at >1 nM. The suppressive effects of gemcitabine on cell proliferation were not potentiated by GV. Overall, the present study demonstrated that GV exhibits a potential suppressive effect on the proliferation of human breast cancer MDA-MB-231 cells in vitro.

  2. Vascular endothelial growth inhibitor affects the invasion, apoptosis and vascularisation in breast cancer cell line MDA-MB-231

    Institute of Scientific and Technical Information of China (English)

    Gao Yinguang; Ge Zhicheng; Zhang Zhongtao; Bai Zhigang; Ma Xuemei; Wang Yu

    2014-01-01

    Background Breast cancer is one of the most common malignant female diseases worldwide.It is a significant threat to every woman's health.Vascular endothelial growth inhibitor (VEGI) is known to be abundant in endothelial cells.According to previous literature,overexpression of VEGI has been shown to inhibit tumor neovascularisation and progression in cellular and animal models,but there has been limited research on the significance of VEGI in the breast cancer.Methods In our study,cell lines MDA-MB-231 were first constructed in which VEGI mediated by lentivirus over-expressed.The effects of VEGI over-expression on MDA-MB-231 cells were investigated both in vitro and in vivo.The expression of VEGI in the MDA-MB-231 cells after infection of lentivirus was analyzed using real-time PCR and Western blotting.The effect of the biological characteristics of MDA-MB-231 cells was assessed by growth,invasion,adhesion,and migration assay with subcutaneous tumor-bearing nude mice models.Then the growth curves of the subcutaneous tumors were studied.Expressions of VEGI,CD31 and CD34 in the tumors were analyzed by immunohistochemistry and apoptosis was detected by flow cytometry and immunohistochemistry.Results Infection of MDA-MB-231 cells within the lentivirus resulted in approximately a 1 000-fold increase in the expression of VEGI.As can be seen in the invasion,adhesion and migration assay,the over-expression of VEGI can inhibit the ability of MDA-MB-231 cells during migration,adhesion and invasion.The volume of the subcutaneous tumor in the over-expression group was distinctly and significantly less than that of the control groups.Immunohistochemistry analysis of the tumor biopsies cleady showed the expression of VEGI in the over-expression group increased while CD31 and CD34 decreased significantly.In vitro and in vivo,the early apoptosis rate and the apoptosis index were increased within the VEGI over-expression group as compared with the control group.Conclusions Taken

  3. 深圳市宝安区肥胖儿童营养知识、态度、行为调查%Bao'an District, Shenzhen, obese child nutrition knowledge, attitude and behavior

    Institute of Scientific and Technical Information of China (English)

    刘西韶; 陈侃; 刘健宏; 李迎芳; 肖巨庆

    2011-01-01

    目的:了解本地区肥胖儿童营养知识、态度和行为(KAP)现状,在肥胖儿童中普及营养知识、改变不良的饮食习惯,提倡平衡膳食.方法:随机整群抽取宝安区10所学校6~12岁学生790名,采取自行设计的调查问卷对肥胖儿童进行有关营养知识、态度、行为调查.结果:营养测试中,营养教育程度越高的儿童成绩越好,大部份儿童的营养态度端正,行为良好,但部分儿童也存在一些问题如:按照口味而不是按照营养需要选择食物,不常吃水果、常吃零食、雪糕等.结论:课堂教育对提高肥胖儿童的营养知识水平有重大作用;在加强课堂营养教育的同时,应注重综合性的宣传教育,以提高儿童的营养知识水平,建立良好的饮食行为.%Objective obese children. Bao'an District, Shenzhen, nutrition knowledge, attitude and behavior (KAP) status for the development of nutrition interventions for obese children to provide evidence. Methods Random cluster sample of 10 schools, Bao'an District, students aged 6 to 12 790, to take self-designed questionnaire about obese children, nutrition knowledge, attitude and behavior. Results Nutrition tests, the higher the level of nutrition education the better results for children, most child nutrition with the right attitude, good behavior, but some children, there are some problems such as: according to taste and not in accordance with the nutritional needs of food choices, do not eat fruit, eat snacks, ice cream. Conclusion nutrition education in obese children should be strengthened to improve the nutritional knowledge, changing dietary behavior and improving the health of obese children.

  4. Complete 400-Mb/s burst-mode data OEIC receiver

    Science.gov (United States)

    MacTaggart, I. Ross; Bendett, Mark; Taylor, Stewart S.

    1993-10-01

    A fully integrated burst-mode GaAs MESFET optoelectronic integrated circuit (OEIC) receiver, 215 mil x 109 mil, has been designed and implemented for point-to-point data links for application as a phased-array antenna controller. The chip provides a low-cost means for passing 400-Mb/s antenna control information using fiber optics with a very low bit-error rate. Approximately 350 source-coupled FET logic gates are present on the chip. A new data coding and timing recovery scheme has been developed which is highly tolerant to jitter over a wide bandwidth. The OEIC employs an on-chip MSM photodiode with 0.12-A/W responsivity measured at 780 nm and was fabricated in a 1.0-micron GaAs MESFET manufacturing technology. The low capacitance semi-insulating GaAs substrate minimizes the coupling between analog and digital circuitry. The circuit operates from a single 5-V supply, consumes 1 W of power, and provides an 8-b CMOS output bus together with various utility flags. Optical sensitivity is estimated at -20 dBm for 10(exp -14) BER. As the chip is fully integrated, there is no means to permit BER testing at a serial port. The BER must therefore be evaluated as part of the whole system.

  5. On the Evaluation of MB-OFDM UWB Interference Effects on a WiMAX Receiver

    Directory of Open Access Journals (Sweden)

    Cano Eduardo

    2010-01-01

    Full Text Available The European Commission has recently adopted specific power spectral density masks for ultra wideband (UWB devices, with detect and avoid capabilities, for coexistence with licensed standards. Under these regulations, a novel approach for analyzing the UWB interference effects on the WiMAX downlink is provided in this paper by means of a novel theoretical computation of the bit error rate (BER, simulation results, and measurements in a conducted modality. New analytical BER expressions for both uncoded and coded WiMAX systems, impaired by a single multiband-OFDM (MB-OFDM UWB interference signal, are obtained in this paper for a Rayleigh fading channel. The BER is expressed in terms of the characteristic function of the interference signal. The maximum permissible interference levels and the signal-to-interference (SIR values, which allow the UWB interference effects to be considered negligible, are estimated in this paper from simulation and measurement results. The analysis considers a WiMAX receiver operating at its minimum sensitivity level. The BER, the symbol error probability (SEP, and the error vector magnitude (EVM of the WiMAX link are the metrics employed to characterize the interference effects for both frequency hopping and nonfrequency hopping UWB interferers.

  6. Improved Laccase Production by Trametes pubescens MB89 in Distillery Wastewaters

    Directory of Open Access Journals (Sweden)

    P. J. Strong

    2011-01-01

    Full Text Available Various culture parameters were optimised for laccase synthesis by Trametes pubescens MB89, including pH, carbon source, nitrogen source, lignocellulosic supplements, and reported inducers. Glucose, in conjunction with a complex nitrogen source at pH 5.0, resulted in the highest laccase yield. Adding ethanol, copper, or 2,5-xylidine prior to inoculation further improved laccase concentrations. The addition of 2,5-xylidine was further investigated with multiple additions applied at varying times. This novel application substantially improved laccase production when applied regularly from inoculation and during the growth phase, and also countered glucose repression of laccase synthesis. Single and multiple factor changes were studied in three distillery wastewaters and a wine lees. A synergistic increase in laccase synthesis was observed with the addition of glucose, copper, and 2,5-xylidine. Single addition of 2,5-xylidine proved most beneficial with distillery wastewaters, while copper addition was most beneficial when using the wine lees as a culture medium.

  7. Optimized Method for Untargeted Metabolomics Analysis of MDA-MB-231 Breast Cancer Cells

    Directory of Open Access Journals (Sweden)

    Amanda L. Peterson

    2016-09-01

    Full Text Available Cancer cells often have dysregulated metabolism, which is largely characterized by the Warburg effect—an increase in glycolytic activity at the expense of oxidative phosphorylation—and increased glutamine utilization. Modern metabolomics tools offer an efficient means to investigate metabolism in cancer cells. Currently, a number of protocols have been described for harvesting adherent cells for metabolomics analysis, but the techniques vary greatly and they lack specificity to particular cancer cell lines with diverse metabolic and structural features. Here we present an optimized method for untargeted metabolomics characterization of MDA-MB-231 triple negative breast cancer cells, which are commonly used to study metastatic breast cancer. We found that an approach that extracted all metabolites in a single step within the culture dish optimally detected both polar and non-polar metabolite classes with higher relative abundance than methods that involved removal of cells from the dish. We show that this method is highly suited to diverse applications, including the characterization of central metabolic flux by stable isotope labelling and differential analysis of cells subjected to specific pharmacological interventions.

  8. Production, extraction and stabilization of lutein from microalga Chlorella sorokiniana MB-1.

    Science.gov (United States)

    Chen, Chun-Yen; Jesisca; Hsieh, Chienyan; Lee, Duu-Jong; Chang, Chien-Hsiang; Chang, Jo-Shu

    2016-01-01

    The efficiencies of extraction and preservation of lutein from microalgae are critical for the success of its commercialization. In this study, lutein was produced by Chlorella sorokiniana MB-1 via semi-batch mixotrophic cultivation. The microalgal biomass with a lutein content of 5.21mg/g was pretreated by bead-beating and high pressure cell disruption methods, and the lutein content was harvested by a reduced pressure extraction method. The effect of pretreatment, pressure, solvent type, extraction time and temperature on lutein recovery was investigated. Using high pressure pretreatment followed by extraction with tetrahydrofuran (THF) as solvent resulted in high lutein recovery efficiencies of 87.0% (20min) and 99.5% (40min) at 850mbar and 25°C. In contrast, using ethanol as the solvent, 86.2% lutein recovery was achieved under 450mbar, 35°C and 40min extraction. The extracted lutein was stabilized in olive oil or sunflower oil with half-lives of 53.1 and 63.8days, respectively. Copyright © 2015 Elsevier Ltd. All rights reserved.

  9. Spin state transitions upon visible and infrared excitation of ferric MbN{sub 3}

    Energy Technology Data Exchange (ETDEWEB)

    Helbing, Jan, E-mail: j.helbing@pci.uzh.ch [Physikalisch-Chemisches Institut, Universitaet Zuerich, Winterthurerstrasse 190, 8057 Zuerich (Switzerland)

    2012-03-02

    Highlights: Black-Right-Pointing-Pointer Visible heme and infrared ligand excitation change the spin state equilibrium of MbN{sub 3}. Black-Right-Pointing-Pointer The difference in ligand binding angle between high and low spin protein was measured. Black-Right-Pointing-Pointer Spin state changes take place on the time scale of vibrational relaxation of heme. Black-Right-Pointing-Pointer Heme vibrations may modulate the electronic and functional properties of the protein. - Abstract: When azide binds to ferric Myoglobin it forms either a low-spin or a high-spin complex, which give rise to two well-separated asymmetric stretch bands of the ligand. Both electronic excitation of the Q-band and vibrational excitation of N{sub 3}{sup -} in the mid-IR lead to a similar ultrafast population redistribution in favor of the high spin configuration, which is characterized by a 8 Degree-Sign reorientation of the ligand transition dipole moment. The more stable low spin complex subsequently re-emerges with a 18 ps time-constant. It is argued that the observed spin state changes are caused by the participation of low-lying electronic excitations in the cooling process of heme.

  10. Precise and in situ genetic humanization of 6 Mb of mouse immunoglobulin genes.

    Science.gov (United States)

    Macdonald, Lynn E; Karow, Margaret; Stevens, Sean; Auerbach, Wojtek; Poueymirou, William T; Yasenchak, Jason; Frendewey, David; Valenzuela, David M; Giallourakis, Cosmas C; Alt, Frederick W; Yancopoulos, George D; Murphy, Andrew J

    2014-04-01

    Genetic humanization, which involves replacing mouse genes with their human counterparts, can create powerful animal models for the study of human genes and diseases. One important example of genetic humanization involves mice humanized for their Ig genes, allowing for human antibody responses within a mouse background (HumAb mice) and also providing a valuable platform for the generation of fully human antibodies as therapeutics. However, existing HumAb mice do not have fully functional immune systems, perhaps because of the manner in which they were genetically humanized. Heretofore, most genetic humanizations have involved disruption of the endogenous mouse gene with simultaneous introduction of a human transgene at a new and random location (so-called KO-plus-transgenic humanization). More recent efforts have attempted to replace mouse genes with their human counterparts at the same genetic location (in situ humanization), but such efforts involved laborious procedures and were limited in size and precision. We describe a general and efficient method for very large, in situ, and precise genetic humanization using large compound bacterial artificial chromosome-based targeting vectors introduced into mouse ES cells. We applied this method to genetically humanize 3-Mb segments of both the mouse heavy and κ light chain Ig loci, by far the largest genetic humanizations ever described. This paper provides a detailed description of our genetic humanization approach, and the companion paper reports that the humoral immune systems of mice bearing these genetically humanized loci function as efficiently as those of WT mice.

  11. Control rod calibration and reactivity effects at the IPEN/MB-01 reactor

    Energy Technology Data Exchange (ETDEWEB)

    Pinto, Letícia Negrão; Gonnelli, Eduardo; Santos, Adimir dos [Nuclear Engineering Center, Nuclear and Energy Research Institute- IPEN/CNEN-SP, Av. Lineu Prestes 2242 - Cidade Universitária - 05508-000 - São Paulo - SP (Brazil)

    2014-11-11

    Researches that aim to improve the performance of neutron transport codes and quality of nuclear cross section databases are very important to increase the accuracy of simulations and the quality of the analysis and prediction of phenomena in the nuclear field. In this context, relevant experimental data such as reactivity worth measurements are needed. Control rods may be made of several neutron absorbing materials that are used to adjust the reactivity of the core. For the reactor operation, these experimental data are also extremely important: with them it is possible to estimate the reactivity worth by the movement of the control rod, understand the reactor response at each rod position and to operate the reactor safely. This work presents a temperature correction approach for the control rod calibration problem. It is shown the control rod calibration data of the IPEN/MB-01 reactor, the integral and differential reactivity curves and a theoretical analysis, performed by the MCNP-5 reactor physics code, developed and maintained by Los Alamos National Laboratory, using the ENDF/B-VII.0 nuclear data library.

  12. Control rod calibration and reactivity effects at the IPEN/MB-01 reactor

    Science.gov (United States)

    Pinto, Letícia Negrão; Gonnelli, Eduardo; dos Santos, Adimir

    2014-11-01

    Researches that aim to improve the performance of neutron transport codes and quality of nuclear cross section databases are very important to increase the accuracy of simulations and the quality of the analysis and prediction of phenomena in the nuclear field. In this context, relevant experimental data such as reactivity worth measurements are needed. Control rods may be made of several neutron absorbing materials that are used to adjust the reactivity of the core. For the reactor operation, these experimental data are also extremely important: with them it is possible to estimate the reactivity worth by the movement of the control rod, understand the reactor response at each rod position and to operate the reactor safely. This work presents a temperature correction approach for the control rod calibration problem. It is shown the control rod calibration data of the IPEN/MB-01 reactor, the integral and differential reactivity curves and a theoretical analysis, performed by the MCNP-5 reactor physics code, developed and maintained by Los Alamos National Laboratory, using the ENDF/B-VII.0 nuclear data library.

  13. A 3.1-4.8 GHz CMOS receiver for MB-OFDM UWB

    Energy Technology Data Exchange (ETDEWEB)

    Yang Guang; Yao Wang; Yin Jiangwei; Zheng Renliang; Li Wei; Li Ning; Ren Junyan, E-mail: w-li@fudan.edu.c [State Key Laboratory of ASIC and System, Fudan University, Shanghai 201203 (China)

    2009-01-15

    An integrated fully differential ultra-wideband CMOS receiver for 3.1-4.8 GHz MB-OFDM systems is presented. A gain controllable low noise amplifier and a merged quadrature mixer are integrated as the RF front-end. Five order Gm-C type low pass filters and VGAs are also integrated for both I and Q IF paths in the receiver. The ESD protected chip is fabricated in a Jazz 0.18 mum RF CMOS process and achieves a maximum total voltage gain of 65 dB, an AGC range of 45 dB with about 6 dB/step, an averaged total noise figure of 6.4 to 8.8 dB over 3 bands and an in-band IIP3 of -5.1 dBm. The receiver occupies 2.3 mm{sup 2} and consumes 110 mA from a 1.8 V supply including test buffers and a digital module.

  14. Study of the Xi(1620) and Xi(1690) resonances in Xi_c -> pi^+ MB decays

    CERN Document Server

    Miyahara, Kenta; Oka, Makoto; Nieves, Juan; Oset, Eulogio

    2016-01-01

    Nonleptonic weak decays of Xi_c into pi^+ and a meson (M)-baryon (B) final state, MB, are analyzed from the viewpoint of probing S=-2 baryon resonances, i.e. Xi(1620) and Xi(1690), which spin-parity and other properties are not well known. We argue that the weak decay of Xi_c is dominated by a single quark-line diagram, preferred by the Cabibbo-Kobayashi-Maskawa coefficient, color recombination factor, the diquark correlation, and the kinematical condition. The decay process has an advantage of being free from meson resonances in the pi^+M invariant mass distribution. The invariant mass distribution of the meson-baryon final state is calculated with three different chiral unitary approaches, assuming that the Xi(1620) and Xi(1690) resonances have J^P=1/2^-. It is found that a clear peak for the Xi(1690) is seen in the piXi and KbarLambda spectra. We also suggest that the ratios of the piXi, KbarLambda and KbarSigma final states are useful to distinguish whether the peak is originated from the Xi(1690) resonan...

  15. Clinical significance of blood gas and electrolyte analysis, CK, CK-MB and HBDH changes in neonatal asphyxia

    Institute of Scientific and Technical Information of China (English)

    Bao-Hua Xu; Xin Lin; Mi-Jia Huang

    2016-01-01

    Objective:To investigate the clinical significance of blood gas and electrolyte analysis, CK, CK-MB and HBDH changes in neonatal asphyxia.Methods:A total of 100 newborns with asphyxia who visited in our hospital were collected, and divided into severe group (n=20) and mild group (n=80) according to the asphyxia degree, and 50 healthy newborns regarded as control group. The 3 groups received blood gas analysis (pH, BE and PaCO2), electrolyte (K+, Na+ and Ca2+) and 3 kinds of enzymes (CK, CK-MB and HBDH) were tested and compared.Results: Compared with control group, pH and BE of blood gas indexes decreased significantly and PaCO2 increased significantly in severe group (P0.05). Compared with mild group, pH and BE of blood gas indexes decreased significantly and PaCO2 increased significantly in severe group (P0.05). Compared with mild group, the level of Ca2+ decreased significantly in severe group (P<0.05); Compared with control group, the levels of CK, CK-MB and HBDH increased significantly in severe and mild group (P<0.05). Compared with mild group, the levels of CK, CK-MB and HBDH increased significantly in severe group (P<0.05).Conclusions:The detection of blood gas and electrolyte analysis, CK, CK-MB and HBDH can provide an objective evidence for the diagnosis of neonatal asphyxia and estimation of severity degree which was helpful in clinical treatment.

  16. Predictors of Left Ventricle Remodeling: Combined Plasma B-type Natriuretic Peptide Decreasing Ratio and Peak Creatine Kinase-MB.

    Science.gov (United States)

    Hsu, Jen-Te; Chung, Chang-Min; Chu, Chi-Ming; Lin, Yu-Shen; Pan, Kuo-Li; Chang, Jung-Jung; Wang, Po-Chang; Chang, Shih-Tai; Yang, Teng-Yao; Jang, Shih-Jung; Yang, Tsung-Han; Hsiao, Ju-Feng

    2017-01-01

    Background: Previous studies reported that patients who had an acute myocardial infarction (AMI) have found that measuring B-type natriuretic peptide (BNP) during the subacute phase of left ventricular (LV) remodeling can predict the possible course of LV remodeling. This study assessed the use of serial BNP serum levels combined with early creatine kinase-MB (CK-MB) to predict the development of significant LV remodeling in AMI patients. Methods: Nighty-seven patients with new onset AMI were assessed using serial echocardiographic studies and serial measurements of BNP levels, both performed on day-2 (BNP1), day-7 (BNP2), day-90 (BNP3), and day-180 (BNP4) after admission. LV remodeling was defined as >20% increase in biplane LV end-diastolic volume on day-180 compared to baseline (day-2). Results: Patients were divided into LV remodeling [LVR(+)] and non LV remodeling [LVR(-)] groups. No first-week BNP level was found to predict remodeling. However, the two groups had significantly different day-90 BNP level (208.1 ± 263.7 pg/ml vs. 82.4 ± 153.7 pg/ml, P = 0.039) and significantly different 3-month BNP decrease ratios ( R BNP13) (14.4 ± 92.2% vs. 69.4 ± 25.9%, P MB (cut-off 48.2 ng/ml; AUC = 0.672; P = 0.014) was another independent predictor of remodeling. Additionally, combining peak CK-MB and R BNP13 offered an excellent discrimination for half-year remodeling when assessed by ROC curve (AUC = 0.818, P MB additionally offered an incremental power to the predictions derived from serial BNP examinations.

  17. j695mb.m77t and j695mb.h77t: MGD77T data and header files for single-beam bathymetry data for field activity J-6-95-MB in Monterey Bay from 10/16/1995 to 11/30/1995

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Single-beam bathymetry data along with DGPS navigation data was collected as part of the U.S. Geological Survey cruise J-6-95-MB. The cruise was conducted from in...

  18. j695mb.m77t and j695mb.h77t: MGD77T data and header files for single-beam bathymetry data for field activity J-6-95-MB in Monterey Bay from 10/16/1995 to 11/30/1995

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Single-beam bathymetry data along with DGPS navigation data was collected as part of the U.S. Geological Survey cruise J-6-95-MB. The cruise was conducted from in...

  19. o399mb.m77t and o399mb.h77t: MGD77T data and header files for single-beam bathymetry data for field activity O-3-99-MB in Point Sur, Monterey Canyon, California from 06/25/1999 to 06/29/1999

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Single-beam bathymetry data along with GPS navigation data was collected as part of the U.S. Geological Survey cruise O-3-99-MB. The cruise was conducted in Point...

  20. p192mb.m77t and p192mb.h77t: MGD77T data and header files for single-beam bathymetry data for field activity P-1-92-MB in Monterey Bay from 03/20/1992 to 03/22/1992

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Single-beam bathymetry data along with SINS navigation data was collected as part of the U.S. Geological Survey cruise P-1-92-MB. The cruise was conducted in...

  1. o399mb.m77t and o399mb.h77t: MGD77T data and header files for single-beam bathymetry data for field activity O-3-99-MB in Point Sur, Monterey Canyon, California from 06/25/1999 to 06/29/1999

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Single-beam bathymetry data along with GPS navigation data was collected as part of the U.S. Geological Survey cruise O-3-99-MB. The cruise was conducted in Point...

  2. j295mb.m77t and j295mb.h77t: MGD77T data and header files for single-beam bathymetry data for field activity J-2-95-MB in Monterey Bay from 03/06/1995 to 04/15/1995

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Single-beam bathymetry data along with DGPS navigation data was collected as part of the U.S. Geological Survey cruise J-2-95-MB. The cruise was conducted from in...

  3. Draft Genome Sequence of Pseudomonas hussainii Strain MB3, a Denitrifying Aerobic Bacterium Isolated from the Rhizospheric Region of Mangrove Trees in the Andaman Islands, India.

    Science.gov (United States)

    Jaiswal, Shubham K; Saxena, Rituja; Mittal, Parul; Gupta, Ankit; Sharma, Vineet K

    2017-02-02

    The genome sequence of Pseudomonas hussainii MB3, isolated from the rhizospheric region of mangroves in the Andaman Islands, is comprised of 3,644,788 bp and 3,159 protein coding genes. Draft genome analysis indicates that MB3 is an aerobic bacterium capable of performing assimilatory sulfate reduction, dissimilatory nitrate reduction, and denitrification.

  4. Contribution of the BacT/Alert MB Mycobacterium bottle to bloodstream infection surveillance in Thailand: added yield for Burkholderia pseudomallei.

    Science.gov (United States)

    Jorakate, Possawat; Higdon, Melissa; Kaewpan, Anek; Makprasert, Sirirat; Yuenprakhon, Somkhit; Tawisaid, Kittisak; Dejsirilert, Surang; Whistler, Toni; Baggett, Henry C

    2015-03-01

    Community-acquired bloodstream infections cause substantial morbidity and mortality worldwide, but microbiology capacity and surveillance limitations have challenged good descriptions of pathogen distribution in many regions, including Southeast Asia. Active surveillance for bloodstream infections has been conducted in two rural Thailand provinces for >7 years. Blood specimens were divided into two culture bottles, one optimized for aerobic growth (F bottle) and a second for enhanced growth of mycobacteria (MB bottle), and processed with the BactT/Alert 3D system. Because the routine use of MB culture bottles is resource intensive (expensive and requires prolonged incubation), we assessed the added yield of MB bottles by comparing the proportion of pathogens detected by MB versus that by F bottles from 2005 to 2012. Of 63,066 blood cultures, 7,296 (12%) were positive for at least one pathogen; the most common pathogens were Escherichia coli (28%), Burkholderia pseudomallei (11%), Klebsiella pneumoniae (9%), and Staphylococcus aureus (6%). Two bottles improved the yield overall, but the added yield attributable to the MB bottles was limited to a few pathogens. In addition to the detection of mycobacteria and some fungi, MB bottles improved the detection of B. pseudomallei (27% [MB] versus 8% [F]; P MB bottles is warranted for patients at risk for mycobacterial and fungal infections and for infection with B. pseudomallei, a common cause of septicemia in Thailand. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  5. Interrelationship of rotavirus infection and Creatine Kinase-MB isoenzyme levels in children hospitalized with acute gastroenteritis in Guangzhou, China, 2012-2015.

    Science.gov (United States)

    Zheng, Jianbin; Zheng, Haiqing; Gupta, Ramit Kumar; Li, Huixian; Shi, Hui; Pan, Liyan; Gong, Sitang; Liang, Huiying

    2017-08-09

    Elevated levels of Creatine Kinase-MB (CK-MB) Isoenzyme are a common phenomenon among rotavirus (RV) diarrhea. However, few studies have addressed this issue using large sample size. In current study, 1,118 children (age MB and its relationship with RV-infection were analyzed and characterized. Multivariate linear regression models showed that RV-positive cases had a 28% rise in CK-MB compared to RV-negative cases (OR = 1.28, 95% CI: 1.15 to 1.41, P MB level of RV-positive group started to rise immediately at the 1(st) day of diarrhea, reached the peak on days 2 to 4, declined during 4-9 days, and then reached a relatively stable level when compared to the RV-negative group. Mediation analyses showed that indirect effect of RV infection on the increase of CK-MB via Vesikari score was significant (β = 8.01, P MB value is a common finding in RV-infection and completely mediated by the severity of diarrhea. CK-MB monitoring may help to identify children with more severe viral infection.

  6. Binding investigation on the interaction between Methylene Blue (MB)/TiO2 nanocomposites and bovine serum albumin by resonance light-scattering (RLS) technique and fluorescence spectroscopy.

    Science.gov (United States)

    Li, Yuesheng; Zhang, Yue; Sun, Shaofa; Zhang, Aiqing; Liu, Yi

    2013-11-05

    The interaction between Methylene Blue (MB)/TiO2 nanocomposites and bovine serum albumin (BSA) was investigated by resonance light scattering (RLS), fluorescence, three-dimension spectra and UV-vis absorbance spectroscopy. Several factors which may influence the RLS intensity were also investigated before characterizing MB/TiO2-BSA complex. It was proved that the mechanism of MB/TiO2 nanocomposites binding to BSA was mainly a result of the formation of MB/TiO2-BSA complex. The binding constant of MB/TiO2-BSA is 0.762 × 10(-5) L mol(-1) at 298K. By calculating the binding constant at different temperature, the thermodynamic parameters ΔH, ΔG, and ΔS can be observed and deduced that the hydrophobic interactions played an important role to stabilize the complex. The distance r (3.73 nm) between donor (BSA) and acceptor (MB/TiO2) was obtained according to fluorescence resonance energy transfer (FRET). The binding site for MB/TiO2 on BSA was mainly located in sub-domain IIA. The UV-vis absorbance, circular dichroism and three dimension fluorescence have also been used to investigate the effect of MB/TiO2 on the conformation of BSA. Copyright © 2013 Elsevier B.V. All rights reserved.

  7. Genome Sequence of Bacillus subtilis MB73/2, a Soil Isolate Inhibiting the Growth of Plant Pathogens Dickeya spp. and Rhizoctonia solani.

    Science.gov (United States)

    Krzyzanowska, Dorota M; Iwanicki, Adam; Ossowicki, Adam; Obuchowski, Michal; Jafra, Sylwia

    2013-05-16

    Bacillus subilis MB73/2 is a Gram-positive bacterium isolated in Poland from a meadow soil sample. When tested in vitro, the strain shows strong antagonism toward plant pathogens-the soft rot-causing bacteria Dickeya spp. and the crown rot fungus Rhizoctonia solani. Here, we present the genome sequence of MB73/2.

  8. Genome Sequence of Bacillus subtilis MB73/2, a Soil Isolate Inhibiting the Growth of Plant Pathogens Dickeya spp. and Rhizoctonia solani

    OpenAIRE

    Krzyzanowska, Dorota M.; Iwanicki, Adam; Ossowicki, Adam; Obuchowski, Michał; Jafra, Sylwia

    2013-01-01

    Bacillus subilis MB73/2 is a Gram-positive bacterium isolated in Poland from a meadow soil sample. When tested in vitro, the strain shows strong antagonism toward plant pathogens—the soft rot-causing bacteria Dickeya spp. and the crown rot fungus Rhizoctonia solani. Here, we present the genome sequence of MB73/2.

  9. 保靖黄金茶产地茶园节肢动物多样性调查%Investigation of Arthropod Diversity in Tea Garden Producing “Bao Jing” Golden Tea

    Institute of Scientific and Technical Information of China (English)

    黄安平; 周凌云

    2011-01-01

    对保靖黄金茶产地10个代表性茶园的节肢动物多样件进行了调查,茶园节肢动物隶属昆虫纲和蛛形纲,昆虫纲以同翅目昆虫数量较多.其中,叶蝉科(Cicadellidae)、广翅蜡蝉科(Rieaniidae)、蛾蜡蝉科(Flatidae)昆虫为优势类群.天敌优势类群为蛛形纲蜘蛛.植物多样性较大的样地多样性指数、均匀度指数、丰富度指数、优势度指数均明显高于其他样地.%Diversity of arthropod in 10 representational tea gardens which producing “Bao Jing” golden tea were investigated. The arthropod in tea garden belongs to Insecta and Arachnida. The homopteran is the most abundant insect in Insecta, in which, Cicadellidae, Ricaniidae and Flatidae were the preponderant populations. Spiders from Arachnida are the main natural enemy of insect pest. The diversity index, evenness index, species richness index and dominance index of plots with larger plant diversity were significantly higher than others.

  10. 健胃喜食宝片促进消化功能动物试验研究%Animal test on "Jian Wei Xi Shi Bao Tablets" in facilitating digestive function

    Institute of Scientific and Technical Information of China (English)

    王叔桥; 张丽宏; 刘莉; 马晶

    2012-01-01

    Objective To evaluate the facilitating digestive function of Jian Wei Xi Shi Bao Tablets in animal. Methods Male KM mice were randomly divided into control group, model control group and three dosage groups(0. 53,1. 06,1. 60g/kg-BW). The dosage groups were orally administered respectively with test drugs for 15 days, both control group and model control group were treated with experimental water. Mice constipation model was established by using compound diphenixylate. Small intestine movement was studied out in mice, and the ink propelling rates was detected. Male SD rats were randomly divided into control group and three dosage groups(0. 265,0. 53,1. 06g/kgBW). The dosage groups were orally administered respectively with test drugs for 30 days. Body weight, weight gain, food intake and food efficiency ratio were observed in rats, and the activity and secretion quantity of pepsin were detected. The control group was treated with experimental water. Results Jian Wei Xi Shi Bao Tablets (1.06, 1. 60g/kgBW) improved significantly the ink propelling rate in small intestine of mice(P <0.01) and the body weight and food intake of rats, with no significant difference in food efficiency ratio. Jian Wei Xi Shi Bao Tablets ( 0. 53 、1.06g / kgBW) aslo significantly enhanced the activity and secretion quantity of pepsin in rats(P < 0.01). Conclusion The experiment result indicates that "Jian Wei Xi Shi Bao Tablets "has promoting degestive function in animals, namely enhance appetite, food intake , the secretion of gastric juice and pepsin, body weight and small intestine peristalsis, and aslo has the defecation function in mice.%目的 研究健胃喜食宝片促进动物消化功能的效果.方法 将昆明种雄性小鼠随机分为空白组、模型对照组和3个剂量(0.53、1.06、1.60g/kgBW)的给药组.各剂量组分别连续经口给予受试样品15d,空白组和模型对照组给予实验用水.用复方地芬诺酯制造小鼠便秘模型,进行小鼠小肠运

  11. 王宝亮教授治疗重症肌无力验案三则%Treating 3 cases of myasthenia gravis by Professor WANG Bao-liang

    Institute of Scientific and Technical Information of China (English)

    文凯华; 牛跃辉; 王宝亮

    2012-01-01

      Professor WANG Bao-liang is a famous doctor of Chinese medicine, clinical experience of 30 years, good at using the integrative medicine to treat cerebrovascular disease, motor neuron disease and multiple sclerosis. In recent years, he focused on the clinical study of myasthenia gravis, notable curative effect. The teacher Wang in the clinical treatment of three cases of myasthenia gravis was summarized.%  王宝亮教授,一位名中医,临证30余载,擅长中西医结合治疗脑血管病、运动神经元病、多发性硬化。近年来专注于重症肌无力的临床研究,疗效显著。兹将王师在临床中治疗重症肌无力之验案三则进行了总结。

  12. Non-Point Source Pollutant Load Variation in Rapid Urbanization Areas by Remote Sensing, Gis and the L-THIA Model: A Case in Bao'an District, Shenzhen, China

    Science.gov (United States)

    Li, Tianhong; Bai, Fengjiao; Han, Peng; Zhang, Yuanyan

    2016-11-01

    Urban sprawl is a major driving force that alters local and regional hydrology and increases non-point source pollution. Using the Bao'an District in Shenzhen, China, a typical rapid urbanization area, as the study area and land-use change maps from 1988 to 2014 that were obtained by remote sensing, the contributions of different land-use types to NPS pollutant production were assessed with a localized long-term hydrologic impact assessment (L-THIA) model. The results show that the non-point source pollution load changed significantly both in terms of magnitude and spatial distribution. The loads of chemical oxygen demand, total suspended substances, total nitrogen and total phosphorus were affected by the interactions between event mean concentration and the magnitude of changes in land-use acreages and the spatial distribution. From 1988 to 2014, the loads of chemical oxygen demand, suspended substances and total phosphorus showed clearly increasing trends with rates of 132.48 %, 32.52 % and 38.76 %, respectively, while the load of total nitrogen decreased by 71.52 %. The immigrant population ratio was selected as an indicator to represent the level of rapid urbanization and industrialization in the study area, and a comparison analysis of the indicator with the four non-point source loads demonstrated that the chemical oxygen demand, total phosphorus and total nitrogen loads are linearly related to the immigrant population ratio. The results provide useful information for environmental improvement and city management in the study area.

  13. 深圳市宝安区2000~2009年孕产妇死亡特征分析%Analysis the characteristics of maternal mortality in Bao'an District of Shenzhen City from 2000 to 2009

    Institute of Scientific and Technical Information of China (English)

    温瑞英; 王维; 黄洁; 李建梅; 王尊; 黄跃华; 叶彩云

    2011-01-01

    目的 分析深圳市宝安区2000~2009年孕产妇死亡的控制效果及特征,为进一步降低孕产妇死亡率提供决策依据.方法 对深圳市宝安区2000~2009年的148例死亡孕产妇监测资料进行回顾性调查,应用SPSS 13.0软件进行统计,分析孕产妇死亡率及相关特征的变化.结果 2000~2009年宝安区共有148例孕产妇死亡,其中流动人口133例(占89.86%).孕产妇死亡率从2000年的133.26/10万下降到2009年的29.16/10万,10年平均死亡率为42.22/10万(其中,流动人口49.57/10万);10年间全人口及流动人口死亡率的变化差异均有统计学意义(x2=49.22,P<0.01;x2=69.20,P<0.01).与前5年相比,后5年孕产妇死因中妊娠期高血压性疾病(7.23%vs 12.31%)、妊娠合并外科性疾病(1.20%vs 7.69%)、肿瘤性疾病(0.00%vs4.61%)、产褥感染(1.20%vs 4.61%)有明显上升趋势;而产科出血(33.74%vs 27.69%)、羊水栓塞(26.51%vs 23.08%)、妊娠合并内科疾病(20.48%vs 9.23%)有所下降.结论 宝安区孕产妇死亡率得到了有效控制,与外来流动人口孕产妇死亡率大幅下降有着密切关系.继续深入开展"降消"项目、加强流动人群孕产妇的管理,仍是宝安区今后妇幼保健工作的重要任务.%Objective To analyze the control outcome and the characteristics of maternal mortality in Bao' an District of Shenzhen City from 2000 to 2009 and to provide evidence of reducing maternal mortality.Methods 148 materhal mortality cases from surveillance data dating from 2000 to 2009 were analyzed by the software SPSS 13.0 finding out the changes of maternal mortality and its characteristics.Results Among 148 cases of maternal mortality in Bao' an District from 2000 to 2009, 133 cases were from the immigrant population ( 89.86% ).Maternal mortality rate dropped from 133.26/100 000 in 2000 to 29.16/100 000 in 2009, the average of maternal mortality was 42.22/100 000 for the whole population and 49.57/100 000 for the immigrant

  14. Tourism resources analysis and ecological construction countermeasures of Bao Taishan National Forest Park%宝台山国家森林公园旅游资源分析与生态建设对策

    Institute of Scientific and Technical Information of China (English)

    佘丽华; 史建; 耿满; 朱国飞; 苏琴

    2012-01-01

    The treasure of BaoTaishan national Forest Park tourism resources on the basis of the quantitative analysis qualitative, and the analysis results are applied to the ecological construction of Forest Park. The organic combination of ecological tourism and forest landscape protection ,witch through the reasonable construction, Contains very important significance for public awareness of ecological protection and quality enhancement, the loss of impact and pressure from the resource and environment of tourist area ecological tourism and the protection and construction of natural traditional tourism to achieve the double purpose of environment.%摘要:对宝台山国家森林公园内的旅游资源进行定性基础上的定量分析,并将分析成果应用于森林公园的生态建设,通过合理的建设将生态旅游与森林景观保护有机的结合起来,对于增强公众的生态保护意识和素养,减轻传统旅游对旅游区环境、资源的冲击和压力,达到生态旅游与自然环境保护和建设的双重目的,具有十分重要的意义。

  15. Sequencing of 6.7 Mb of the melon genome using a BAC pooling strategy

    Directory of Open Access Journals (Sweden)

    Garcia-Mas Jordi

    2010-11-01

    Full Text Available Abstract Background Cucumis melo (melon belongs to the Cucurbitaceae family, whose economic importance among horticulture crops is second only to Solanaceae. Melon has a high intra-specific genetic variation, morphologic diversity and a small genome size (454 Mb, which make it suitable for a great variety of molecular and genetic studies. A number of genetic and genomic resources have already been developed, such as several genetic maps, BAC genomic libraries, a BAC-based physical map and EST collections. Sequence information would be invaluable to complete the picture of the melon genomic landscape, furthering our understanding of this species' evolution from its relatives and providing an important genetic tool. However, to this day there is little sequence data available, only a few melon genes and genomic regions are deposited in public databases. The development of massively parallel sequencing methods allows envisaging new strategies to obtain long fragments of genomic sequence at higher speed and lower cost than previous Sanger-based methods. Results In order to gain insight into the structure of a significant portion of the melon genome we set out to perform massive sequencing of pools of BAC clones. For this, a set of 57 BAC clones from a double haploid line was sequenced in two pools with the 454 system using both shotgun and paired-end approaches. The final assembly consists of an estimated 95% of the actual size of the melon BAC clones, with most likely complete sequences for 50 of the BACs, and a total sequence coverage of 39x. The accuracy of the assembly was assessed by comparing the previously available Sanger sequence of one of the BACs against its 454 sequence, and the polymorphisms found involved only 1.7 differences every 10,000 bp that were localized in 15 homopolymeric regions and two dinucleotide tandem repeats. Overall, the study provides approximately 6.7 Mb or 1.5% of the melon genome. The analysis of this new data has

  16. A YAC contig of approximately 3 Mb from human chromosome 5q31 [yields] q33

    Energy Technology Data Exchange (ETDEWEB)

    Li, Xiang; Wang Jabs, E.; Hawkins, A.L.; Griffin, C.A. (John Hopkins School of Medicine, Baltimore, MD (United States)); Wise, C.A.; Lovett, M. (Univ. of Texas Southwestern Medical Center, Dallas, TX (United States)); Le Paslier, D. (CEPH, Paris (France)); Pittler, S.J. (Univ. of South Alabama College of Medicine, Mobile, AL (United States))

    1994-02-01

    The human chromosome 5q31-q33 region contains an interesting cluster of growth factor and receptor genes. In addition, several genetic disease loci have been localized within this region, but have not as yet been isolated as molecular clones. These include those loci involved in autosomal dominant limb-girdle muscular dystrophy, diastrophic dysplasia, Treacher Collins syndrome, and myeloid disorders associated with the 5q-syndrome. A yeast artificial chromosome (YAC) contig of this region would assist in the further localization and isolation of these genes. The authors have used YACs isolated from the Washington University and Centre d'Etude du Polymorphisme Humain YAC libraries, including YACs from the large insert (mega) YAC library to build a contig greater than 3 Mb in size. An STS content strategy coupled with limited walking from YAC ends was used to isolate 22 overlapping YACs with as much as sixfold coverage. A total of 20 STSs, derived from genes, anonymous sequences, and vector Alu-PCR or inverse PCR products, were used to compile this contig. The order of loci, centromere-GRL-D5S207-D5S70-D5S545-D5S546-D5S547-D5S68-D5S548-D5S210-D5S549-D5S686- ADRB2-D5S559-CSF1R-D5S551-RPS14-D5S519-SPARC-telomere, was derived from the overlapping clones. This contig and clones derived from it will be useful substrates in selecting candidate cDNAs for the disease loci in this interval. 45 refs., 1 fig., 2 tabs.

  17. High speed Radix-4 soft-decision Viterbi decoder for MB-OFDM UWB system

    Science.gov (United States)

    Liang, Guixuan; Portilla, Jorge; Riesgo, Teresa

    2013-05-01

    In this paper, a 64 state soft decision Viterbi Decoder (VD) system by using a high speed radix-4 Add Compare Select (ACS) architecture is presented. The proposed VD system can support different data rate (from 53.5 Mbps to 480 Mbps) for Multiband Orthogonal Frequency-division Multiplexing (MB-OFDM) Ultra-Wideband (UWB) system when implemented onto the FPGA board. The proposed VD employs efficient two steps Radix 4 architecture, which is responsible of calculating two steps of 64 state Radix 4 Branch Metrics (BM) within one clock cycle. The branch metrics are calculated using a uniform distance measurement algorithm, which equals to the symbol itself when compared to logic-0 and equal to its one's complement when compared to logic-1. By employing the modified Modulo Normalization algorithm, it is possible to use only a 10- bit memory block to restore each of the 64 state metrics, with the advantage of avoiding errors caused by overflow during the updating process for state metrics, and simplifying the comparator circuit of the ACS unit. The Two Pointer Even Algorithm, which is considered to be very simple and more hardware-efficient than the register exchange algorithm, is used for tracing back the survivor sequence and output the decoded data stream. 3-bit soft decision input sequences are used for gathering the experimental results. The sampling frequency of the MBOFDM UWB system is 528 MHz, by using the proposed two steps Radix 4 VD architecture we can process 4 input signals in parallel within one clock cycle, therefore only 132 MHz operating frequency is needed for the proposed VD system. This will dramatically reduce the dynamic power consumption for hardware implementation. Final results of the implementation show that the proposed VD architecture can support a maximum working frequency of 152.5 MHz on Xilinx XUPV5-LX110T Evaluation Platform.

  18. MB109 as bioactive human bone morphogenetic protein-9 refolded and purified from E. coli inclusion bodies.

    Science.gov (United States)

    Kuo, Mario Meng-Chiang; Nguyen, Phuong Hong; Jeon, Yun-Hui; Kim, Subin; Yoon, So-Mi; Choe, Senyon

    2014-02-24

    The development of chemical refolding of transforming growth factor-beta (TGF-β) superfamily ligands has been instrumental to produce the recombinant proteins for biochemical studies and exploring the potential of protein therapeutics. The osteogenic human bone morphogenetic protein-2 (hBMP-2) and its Drosophila DPP homolog were the early successful cases of refolding into functional form. Despite the similarity in their three dimensional structure and amino acid sequences, several other TGF-β superfamily ligands could not be refolded readily by the same methods. Here, we report a comprehensive study on the variables of a rapid-dilution refolding method, including the concentrations of protein, salt, detergent and redox agents, pH, refolding duration and the presence of aggregation suppressors and host-cell contaminants, in order to identify the optimal condition to refold human BMP-9 (hBMP-9). To produce a recombinant form of hBMP-9 in E. coli cells, a synthetic codon-optimized gene was designed to encode the mature domain of hBMP-9 (Ser320 - Arg429) directly behind the first methionine, which we herein referred to as MB109. An effective purification scheme was also developed to purify the refolded MB109 to homogeneity with a final yield of 7.8 mg from 100 mg of chromatography-purified inclusion bodies as a starting material. The chemically refolded MB109 binds to ALK1, ActRIIb and BMPRII receptors with relatively high affinity as compared to other Type I and Type II receptors based on surface plasmon resonance analysis. Smad1-dependent luciferase assay in C2C12 cells shows that the MB109 has an EC50 of 0.61 ng/mL (25 pM), which is nearly the same as hBMP-9. MB109 is prone to be refolded as non-functional dimer and higher order multimers in most of the conditions tested, but bioactive MB109 dimer can be refolded with high efficiency in a narrow window, which is strongly dependent on the pH, refolding duration, the presence of aggregation suppressors and the

  19. Combination of cisplatin and bromelain exerts synergistic cytotoxic effects against breast cancer cell line MDA-MB-231 in vitro.

    Science.gov (United States)

    Pauzi, Ahmad Zaim Mat; Yeap, Swee Keong; Abu, Nadiah; Lim, Kian Lam; Omar, Abdul Rahman; Aziz, Suraini Abdul; Chow, Adam Leow Thean; Subramani, Tamilselvan; Tan, Soon Guan; Alitheen, Noorjahan Banu

    2016-01-01

    Bromelain, which is a cysteine endopeptidase commonly found in pineapple stems, has been investigated as a potential anti-cancer agent for the treatment of breast cancer. However, information pertaining to the effects of combining bromelain with existing chemotherapeutic drugs remains scarce. This study aimed to investigate the possible synergistic cytotoxic effects of using bromelain in combination with cisplatin on MDA-MB-231 human breast cancer cells. MDA-MB-231 cells were treated with different concentrations (0.24-9.5 µM) of bromelain or cisplatin alone, as well as four different combinations of these two agents to assess their individual and combination effects after 24 and 48 h. Cell viability was analyzed using an MTT assay. The induction of apoptosis was assessed using cell cycle analysis and an Annexin V-FITC assay. The role of the mitochondrial membrane potential in the apoptotic process was assessed using a JC-1 staining assay. Apoptotic protein levels were assessed by western blot analysis and proteome profiling using an antibody array kit. Single-agent treatment with cisplatin or bromelain led to dose- and time-dependent decreases in the viability of the MDA-MB-231 cells at 24 and 48 h. Furthermore, most of the combinations evaluated in this study displayed synergistic effects against MDA-MB-231 cells at 48 h, with combination 1 (bromelain 2 µM + cisplatin 1.5 µM) exhibiting the greatest synergistic effect (P = 0.000). The results of subsequent assays indicated that combination 1 treatment induced apoptosis via mitochondria-mediated pathway. Combination 1 also resulted in significant decreases in the levels of several apoptotic proteins such as Bcl-x and HSP70, compared with bromelain (P = 0.002 and 0.000, respectively) or cisplatin (P = 0.000 and 0.001, respectively) single treatment. Notably, MDA-MB-231 cells treated with combination 1 showed increased levels of the pro-apoptotic proteins Bax compared with those treated with

  20. Dillenia suffruticosa dichloromethane root extract induced apoptosis towards MDA-MB-231 triple-negative breast cancer cells.

    Science.gov (United States)

    Foo, Jhi Biau; Saiful Yazan, Latifah; Tor, Yin Sim; Wibowo, Agustono; Ismail, Norsharina; Armania, Nurdin; Cheah, Yoke Kqueen; Abdullah, Rasedee

    2016-07-01

    Dillenia suffruticosa is traditionally used for treatment of cancerous growth including breast cancer in Malaysia. Dillenia suffruticosa is a well-known medicinal plant in Malaysia for the treatment of cancer. Nevertheless, no study has been reported the cytotoxicity of this plant towards MDA-MB-231 triple-negative breast cancer cells. The present study was designed to investigate the mode of cell death and signalling pathways of MDA-MB-231 cells treated with dichloromethane Dillenia suffruticosa root extract (DCM-DS). Extraction of Dillenia suffruticosa root was performed by the use of sequential solvent procedure. The cytotoxicity of DCM-DS was determined by using MTT assay. The mode of cell death was evaluated by using an inverted light microscope and flow cytometry analysis using Annexin-V/PI. Cell cycle analysis and measurement of reactive oxygen species level were performed by using flow cytometry. The cells were treated with DCM-DS and antioxidants α-tocopherol or ascorbic acid to evaluate the involvement of ROS in the cytotoxicity of DCM-DS. Effect of DCM-DS on the expression of antioxidant, apoptotic, growth, survival genes and proteins were analysed by using GeXP-based multiplex system and Western blot, respectively. The cytotoxicity of compounds isolated from DCM-DS was evaluated towards MDA-MB-231 cells using MTT assay. DCM-DS induced apoptosis, G2/M phase cell cycle arrest and oxidative stress in MDA-MB-231 cells. The induction of apoptosis in MDA-MB-231 cells by DCM-DS is possibly due to the activation of pro-apoptotic JNK1 and down-regulation of anti-apoptotic ERK1, which in turn down-regulates anti-apoptotic BCL-2 to increase the BAX/BCL-2 ratio to initiate the mitochondrial apoptotic pathway. The cell cycle arrest in DCM-DS-treated MDA-MB-231 cells is possibly via p53-independent but p21-dependent pathway. A total of 3 triterpene compounds were isolated from DCM-DS. Betulinic acid appears to be the most major and most cytotoxic compound in DCM

  1. Stable transfection of estrogen receptor-alpha suppresses expression of cyclooxygenase-2 and vascular endothelial growth factor-C in MDA-MB-231 breast cancer cells

    Institute of Scientific and Technical Information of China (English)

    ZHANG Hui; LIN Ying; XIAO Ying; WANG San-ming; LIU Xiang-xia; WANG Shen-ming

    2010-01-01

    Background Estrogen receptor (ER)-negative breast cancer cells are more aggressive than ER-positive cells. Elevated levels of cyclooxygenase-2 (COX-2) and vascular endothelial growth factor-C (VEGF-C) expression have been detected in cultured human breast cancer cells and are associated with negative hormone receptor status. In this study, we created ERα stable transfectants in MDA-MB-231 cells to explore the effect of ERα on cell growth and COX-2 and VEGF-C expression.Methods The green fluorescent protein (GFP)-ERα plasmids were stably transfected into ER-negative MDA-MB-231 cells. The proliferation and migration of untransfected MDA-MB-231 cells, ERα-transfected MDA-MB-231 cells and ER-positive MCF-7 cells were determined. The expression of COX-2, and the levels of VEGF-C mRNA and the VEGF-C secretion concentration were assayed in these cell lines.Results The proliferation and migration capacities of ERα-tranfected MDA-MB-231 cells were significantly decreased (P <0.05). The expression of COX-2 was significantly lower in ERa-tranfected MDA-MB-231 cells than in untranfected MDA-MB-231 cells. The mRNA and protein levels of VEGF-C were lower in ERa-tranfected MDA-MB-231 cells than in untransfected MDA-MB-231 cells (P<0.05).Conclusions ERα stable transfection inhibits proliferation and migration capacities of MDA-MB-231 cells and decreases expression of COX-2 and VEGF-C. The decreases of proliferation and migration capacities may be related to suppression of COX-2 and VEGF-C expression.

  2. A de novo 1.58 Mb deletion, including MAP2K6 and mapping 1.28 Mb upstream to SOX9, identified in a patient with Pierre Robin sequence and osteopenia with multiple fractures.

    Science.gov (United States)

    Smyk, Marta; Roeder, Elizabeth; Cheung, Sau Wai; Szafranski, Przemyslaw; Stankiewicz, Paweł

    2015-08-01

    Defects of long-range regulatory elements of dosage-sensitive genes represent an under-recognized mechanism underlying genetic diseases. Haploinsufficiency of SOX9, the gene essential for development of testes and differentiation of chondrocytes, results in campomelic dysplasia, a skeletal malformation syndrome often associated with sex reversal. Chromosomal rearrangements with breakpoints mapping up to 1.6 Mb up- and downstream to SOX9, and disrupting its distant cis-regulatory elements, have been described in patients with milder forms of campomelic dysplasia, Pierre Robin sequence, and sex reversal. We present an ∼1.58 Mb deletion mapping ∼1.28 Mb upstream to SOX9 that encompasses its putative long-range cis-regulatory element(s) and MAP2K6 in a patient with Pierre Robin sequence and osteopenia with multiple fractures. Low bone mass panel testing using massively parallel sequencing of 23 nuclear genes, including COL1A1 and COL1A2 was negative. Based on the previous mouse model of Map2k6, suggesting that Sox9 is likely a downstream target of the p38 MAPK pathway, and our previous chromosome conformation capture-on-chip (4C) data showing potential interactions between SOX9 promoter and MAP2K6, we hypothesize that deletion of MAP2K6 might have affected SOX9 expression and contributed to our patient's phenotype.

  3. Eleven X chromosome breakpoints associated with premature ovarian failure (POF) map to a 15-Mb YAC contig spanning Xq21.

    Science.gov (United States)

    Sala, C; Arrigo, G; Torri, G; Martinazzi, F; Riva, P; Larizza, L; Philippe, C; Jonveaux, P; Sloan, F; Labella, T; Toniolo, D

    1997-02-15

    Eleven balanced X-autosome translocations associated with premature ovarian failure (POF) were mapped to a YAC contig spanning most of Xq21 and constructed between the DXS223 and DXS1171 loci. The contig corresponds to a genomic region of about 15 Mb and contains the whole X-Y homologous region. The most proximal and most distal breakpoints associated with POF were mapped 15 Mb apart. The remaining breakpoints were localized along this large region, in the X-specific and in the X-Y homologous region. Four of the YACs contained two breakpoints in the same or in flanking STS intervals. Our results confirm the cytological findings and suggest that a minimum number of eight different genes in Xq21 may be involved with ovary development. Interruption of such loci could be the cause of POF.

  4. Association of single nucleotide polymorphisms in CAPN1, CAST and MB genes with meat color of Brahman and crossbreed cattle.

    Science.gov (United States)

    Castro, Susan; Ríos, Marcela; Ortiz, Yurany; Manrique, Carlos; Jiménez, Ariel; Ariza, Fernando

    2016-07-01

    The objective of this research was to determine the association of SNPs in the candidate genes Calpain (CAPN1), Calpastatin (CAST) and Myoglobin (MB) with colorimetric parameters (L *, a *, b *, C *, hue) in a F1 population (n = 164) obtained from crossing Bos taurus × Bos indicus and Bos indicus × Bos indicus. SNPs were analyzed using PCR-RFLP and SSCP. Colorimetric measurements were performed in the muscles Longissimus thoracis et lumborum (LTL) and Semitendinosus (ST) at 7, 14 and 21 days postmortem applying the methodology CIE L* a* b*. The CAST gene showed a significant effect on the b* and hue* parameters in both muscles. MB gene showed significant association with all colorimetric parameters in both LTL and ST muscles, except with b* parameter. The CAPN1 gene did not show any significant association. These results suggest an important role of genetics in meat color variation for cattle raised under the tropic conditions.

  5. Matching coefficients for alpha_s and m_b to O(alpha_s^2) in the MSSM

    CERN Document Server

    Bauer, A; Salomon, J

    2009-01-01

    We compute the exact two-loop matching coefficients for the strong coupling constant alpha_s and the bottom-quark mass m_b within the Minimal Supersymmetric Standard Model (MSSM), taking into account O(alpha_s^2) contributions from Supersymmetric Quantum Chromodynamics (SQCD). We find that the explicit mass pattern of the supersymmetric particles has a significant impact on the predictions of alpha_s and m_b at high energies. Further on, the three-loop corrections exceed the uncertainty due to the current experimental accuracy. In case of the the running bottom-quark mass, they can reach in the large tan(beta) regime up to 30% from the tree-level value.

  6. PEA3 activates CXCR4 transcription in MDA-MB-231 and MCF7 breast cancer cells

    Institute of Scientific and Technical Information of China (English)

    Shengmei Gu; Li Chen; Qi Hong; Tingting Yan; Zhigang Zhuang; Qiaoqiao wang; Wei Jin; Hua Zhu; Jiong Wu

    2011-01-01

    CXC chemokine receptor 4 (CXCR4) is a cell surface receptor that has been shown to mediate the metastasis of many solid tumors including lung,breast,kidney,and prostate tumors.In this study,we found that overexpression of ets variant gene 4 (PEA3) could elevate CXCR4 mRNA level and CXCR4 promoter activity in human MDA-MB-231 and MCF-7 breast cancer cells.PEA3 promoted CXCR4 expression and breast cancer metastasis.Chromatin immunoprecipitation assay demonstrated that PEA3 could bind to the CXCR4 promoter in the cells transfected with PEA3 expression vector.PEA3 siRNA attenuated CXCR4 promoter activity and the binding of PEA3 to the CXCR4 promoter in MDA-MB-231 and MCF-7 cells.These results indicated that PEA3 could activate CXCR4 promoter transcription and promote breast cancer metastasis.

  7. Inhibitory effects of ginseng sapogenins on the proliferation of triple negative breast cancer MDA-MB-231 cells.

    Science.gov (United States)

    Kwak, Jin Ho; Park, Jun Yeon; Lee, Dahae; Kwak, Jae Young; Park, Eun Hwa; Kim, Ki Hyun; Park, Hye-Jin; Kim, Hyun Young; Jang, Hyuk Jai; Ham, Jungyeob; Hwang, Gwi Seo; Yamabe, Noriko; Kang, Ki Sung

    2014-12-01

    Because of poor prognosis, clinical treatment of triple-negative (TN) breast cancer remains the most challenging factor in cancer treatment. Extensive research into alternative cancer therapies includes studying the naturopathic effects of the medicinal herb ginseng. This study investigates the anti-neoplastic properties of ginseng sapogenins and the derivatives: (1) (20(S)-protopanaxadiol (PPD), (2) 20(S)-protopanaxatriol), (3) (20(S)-dihydroprotopanaxadiol, and (4) 20(S)-dihydroprotopanaxatriol). These compounds were found to prevent the proliferation of MDA-MB-231 human breast cancer cells. PPD was the most potent inhibitor, exhibiting an IC₅₀ (5.87 μM) comparable to that of the chemotherapeutic drug taxol. Furthermore, PPD induced dose-dependent cleavage of caspase-8, caspase-3, and PARP in MDA-MB-231 cells. Thus, we propose that PPD acts as anti-cancer agent by stimulating caspase-dependent apoptosis in breast cancer cells.

  8. SN 2010mb: Direct evidence for a supernova interacting with a large amount of hydrogen-free circumstellar material

    Energy Technology Data Exchange (ETDEWEB)

    Ben-Ami, Sagi; Gal-Yam, Avishay; Rabinak, Itay; Yaron, Ofer; Arcavi, Iair; Ofek, Eran O. [Department of Particle Physics and Astrophysics, The Weizmann Institute of Science, Rehovot 76100 (Israel); Mazzali, Paolo A. [Astrophysics Research Institute, Liverpool John Moores University. Liverpool L3 5RF (United Kingdom); Gnat, Orly [Racah Institute of Physics, The Hebrew University, 91904 Jerusalem (Israel); Modjaz, Maryam [Center for Cosmology and Particle Physics, Department of Physics, New York University, 4 Washington Place, room 529, New York, NY 10003 (United States); Sullivan, Mark [Department of Physics (Astrophysics), University of Oxford, DWB, Keble Road, Oxford, OX1 3RH (United Kingdom); Bildsten, Lars [Kavli Institute for Theoretical Physics and Department of Physics Kohn Hall, University of California, Santa Barbara, CA 93106 (United States); Poznanski, Dovi [School of Physics and Astronomy, Tel-Aviv University, Tel Aviv 69978 Israel (Israel); Bloom, Joshua S.; Nugent, Peter E. [Department of Astronomy, University of California, Berkeley, CA 94720-3411 (United States); Horesh, Assaf; Kulkarni, Shrinivas R.; Perley, Daniel [Cahill Center for Astrophysics, California Institute of Technology, Pasadena, CA 91125 (United States); Kasliwal, Mansi M. [Observatories of the Carnegie Institution for Science, 813 Santa Barbara Street, Pasadena, CA 91101 (United States); Quimby, Robert [Kavli IPMU, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa-shi, Chiba 277-8583 (Japan); Xu, Dong, E-mail: sagi.ben-ami@weizmann.ac.il [Dark Cosmology Centre, Niels Bohr Institute, University of Copenhagen, Juliane Maries Vej 30, DK-2100 Copenhagen (Denmark)

    2014-04-10

    We present our observations of SN 2010mb, a Type Ic supernova (SN) lacking spectroscopic signatures of H and He. SN 2010mb has a slowly declining light curve (LC) (∼600 days) that cannot be powered by {sup 56}Ni/{sup 56}Co radioactivity, the common energy source for Type Ic SNe. We detect signatures of interaction with hydrogen-free circumstellar material including a blue quasi-continuum and, uniquely, narrow oxygen emission lines that require high densities (∼10{sup 9} cm{sup –3}). From the observed spectra and LC, we estimate that the amount of material involved in the interaction was ∼3 M {sub ☉}. Our observations are in agreement with models of pulsational pair-instability SNe described in the literature.

  9. Structural and mechanical properties of transition metal borides Nb2MB2 (M=Tc, Ru, and Os) under pressure

    Science.gov (United States)

    Li, Xiaofeng; Yan, Haiyan; Wei, Qun

    2016-10-01

    First-principle total energy calculations are employed to provide a fundamental understanding of the structural, mechanical, and electronic properties of transition metal borides Nb2MB2 (M=Tc, Ru, and Os) within the tetragonal superstructure P4/mnc structure. The mechanically and dynamically stabilities of three borides have been demonstrated by the elastic constants and phonons calculations under pressure. Among these three compounds, Nb2TcB2 exhibits the biggest bulk and Young's modulus, smallest Poission's ratio, and highest harness. Density of states of them revealed that the strong B-B, Nb-B and M-B covalent bonds are major driving forces for their high bulk and shear moduli as well as small Poisson's ratio.

  10. Utilization of 100 mb midlatitude height fields as an indicator of sampling effects on total ozone variations

    Science.gov (United States)

    Miller, A. J.; Nagatani, R. M.; Laver, J. D.; Korty, B.

    1979-01-01

    Midlatitude 100-mb height fields are employed to determine the effects of ground based sampling locations on measurements of variations in the total ozone content of the atmosphere. The precision of the zonal average heights computed by the technique of Angell and Korshover (1978) from data over ozone sampling areas at 50 deg N is compared to the zonal average computed from the entire data set. Linear regressions of ozone contents determined by an analysis of backscatter UV satellite data with respect to 100 mb heights are utilized to transform zonal differences in height to ozone levels. The zonal average total ozone sampling error is found to be on the order of 2% for midlatitudes of the Northern hemisphere, indicating that the general shape of ozone trends determined by ground-based observations appears to be real and the increase of ozone from the mid-1960's to the early 1970's may be greater than previously suggested.

  11. Regulation of MDA-MB-231 cell proliferation by GSK-3β involves epigenetic modifications under high glucose conditions

    Energy Technology Data Exchange (ETDEWEB)

    Gupta, Chanchal; Kaur, Jasmine; Tikoo, Kulbhushan, E-mail: tikoo.k@gmail.com

    2014-05-15

    Hyperglycemia is a critical risk factor for development and progression of breast cancer. We have recently reported that high glucose induces phosphorylation of histone H3 at Ser 10 as well as de-phosphorylation of GSK-3β at Ser 9 in MDA-MB-231 cells. Here, we elucidate the mechanism underlying hyperglycemia-induced proliferation in MDA-MB-231 breast cancer cells. We provide evidence that hyperglycemia led to increased DNA methylation and DNMT1 expression in MDA-MB-231 cells. High glucose condition led to significant increase in the expression of PCNA, cyclin D1 and decrease in the expression of PTPN 12, p21 and PTEN. It also induced hypermethylation of DNA at the promoter region of PTPN 12, whereas hypomethylation at Vimentin and Snail. Silencing of GSK-3β by siRNA prevented histone H3 phosphorylation and reduced DNMT1 expression. We show that chromatin obtained after immunoprecipitation with phospho-histone H3 was hypermethylated under high glucose condition, which indicates a cross-talk between DNA methylation and histone H3 phosphorylation. ChIP-qPCR analysis revealed up-regulation of DNMT1 and metastatic genes viz. Vimentin, Snail and MMP-7 by phospho-histone H3, which were down-regulated upon GSK-3β silencing. To the best of our knowledge, this is the first report which shows that interplay between GSK-3β activation, histone H3 phosphorylation and DNA methylation directs proliferation of breast cancer cells. - Highlights: • High glucose induces phosphorylation of histone H3 and dephosphorylation of GSK-3β. • Moreover, hyperglycemia also leads to increased DNA methylation in MDA-MB-231 cells. • Inhibition of GSK-3β prevented histone H3 phosphorylation and reduced DNMT1 levels. • Interplay exists between GSK-3β, histone H3 phosphorylation and DNA methylation.

  12. Roscovitine regulates invasive breast cancer cell (MDA-MB231) proliferation and survival through cell cycle regulatory protein cdk5.

    Science.gov (United States)

    Goodyear, Shaun; Sharma, Mahesh C

    2007-02-01

    Roscovitine, a purine analogue, has been considered for the treatment of cancer. Anti-cancer therapeutic efficacy is being evaluated in clinical trials. However, the mechanisms remain unclear. In the present study, cyclic-dependent kinase 5 (cdk5) proved to be a molecular target for roscovitine-triggered apoptosis for highly invasive breast cancer cell death. Because our previous studies have shown a potential role of cdk5 in endothelial cell proliferation/apoptosis [Sharma, M.R., Tuszynski, G.P., Sharma, M.C. (2004). Angiostatin-induced inhibition of endothelial cell proliferation/apoptosis is associated with the down-regulation of cell cycle regulatory protein cdk5. J. Cell Biochem. 91, 398-409], here we not only demonstrate first that Cdk5, p35, and p25 proteins were all expressed in invasive breast cancer cells MDA-MB231 but also showed that cdk5 expression regulates MDA-MB231 cell proliferation. In addition, potent mitogen bFGF up-regulates cdk5 expression. Roscovitine specifically inhibits cdk5 expression/activity in a dose-dependent manner with concomitant inhibition of MDA-MB231 cell proliferation and induction of apoptosis. By contrast, the roscovitine analog olomoucine, a specific inhibitor of cdk4, failed to affect MDA-MB231 cell proliferation and apoptosis which implies the specific involvement of cdk5 in roscovitine-triggered cell death/proliferation. Additionally, roscovitine-mediated inhibition of proliferation is irreversible. These data suggest that cdk5 may have a significant role in the regulation of breast cancer cell proliferation and apoptosis and extend beyond its role in neurogenesis. These results suggest that Cdk5 is a novel player in roscovitine-triggered breast cancer cell apoptosis and inhibition of proliferation, therefore, may be a potential therapeutic target.

  13. Antitumor Activity of Chinese Propolis in Human Breast Cancer MCF-7 and MDA-MB-231 Cells

    Directory of Open Access Journals (Sweden)

    Hongzhuan Xuan

    2014-01-01

    Full Text Available Chinese propolis has been reported to possess various biological activities such as antitumor. In present study, anticancer activity of ethanol extract of Chinese propolis (EECP at 25, 50, 100, and 200 μg/mL was explored by testing the cytotoxicity in MCF-7 (human breast cancer ER(+ and MDA-MB-231 (human breast cancer ER(− cells. EECP revealed a dose- and time-dependent cytotoxic effect. Furthermore, annexin A7 (ANXA7, p53, nuclear factor-κB p65 (NF-κB p65, reactive oxygen species (ROS levels, and mitochondrial membrane potential were investigated. Our data indicated that treatment of EECP for 24 and 48 h induced both cells apoptosis obviously. Exposure to EECP significantly increased ANXA7 expression and ROS level, and NF-κB p65 level and mitochondrial membrane potential were depressed by EECP dramatically. The effects of EECP on p53 level were different in MCF-7 and MDA-MB-231 cells, which indicated that EECP exerted its antitumor effects in MCF-7 and MDA-MB-231 cells by inducing apoptosis, regulating the levels of ANXA7, p53, and NF-κB p65, upregulating intracellular ROS, and decreasing mitochondrial membrane potential. Interestingly, EECP had little or small cytotoxicity on normal human umbilical vein endothelial cells (HUVECs. These results suggest that EECP is a potential alternative agent on breast cancer treatment.

  14. Gene cloning and nucleotide sequencing and properties of a cocaine esterase from Rhodococcus sp. strain MB1.

    Science.gov (United States)

    Bresler, M M; Rosser, S J; Basran, A; Bruce, N C

    2000-03-01

    A strain of Rhodococcus designated MB1, which was capable of utilizing cocaine as a sole source of carbon and nitrogen for growth, was isolated from rhizosphere soil of the tropane alkaloid-producing plant Erythroxylum coca. A cocaine esterase was found to initiate degradation of cocaine, which was hydrolyzed to ecgonine methyl ester and benzoate; both of these esterolytic products were further metabolized by Rhodococcus sp. strain MB1. The structural gene encoding a cocaine esterase, designated cocE, was cloned from Rhodococcus sp. strain MB1 genomic libraries by screening recombinant strains of Rhodococcus erythropolis CW25 for growth on cocaine. The nucleotide sequence of cocE corresponded to an open reading frame of 1,724 bp that codes for a protein of 574 amino acids. The amino acid sequence of cocaine esterase has a region of similarity with the active serine consensus of X-prolyl dipeptidyl aminopeptidases, suggesting that the cocaine esterase is a serine esterase. The cocE coding sequence was subcloned into the pCFX1 expression plasmid and expressed in Escherichia coli. The recombinant cocaine esterase was purified to apparent homogeneity and was found to be monomeric, with an M(r) of approximately 65,000. The apparent K(m) of the enzyme (mean +/- standard deviation) for cocaine was measured as 1.33 +/- 0.085 mM. These findings are of potential use in the development of a linked assay for the detection of illicit cocaine.

  15. A 1.6-Mb P1-based physical map of the Down syndrome region on chromosome 21

    Energy Technology Data Exchange (ETDEWEB)

    Ohira, Miki; Suzuki, Kazunobu [National Cancer Center Research Institute, Tokyo (Japan)]|[Kazusa DNA Research Institute, Chiba (Japan); Ichikawa, Hitoshi [National Cancer Center Research Institute, Tokyo (Japan)] [and others

    1996-04-01

    The Down Syndrome (DS) region on chromosome 21, which is responsible for the main features of DS such as characteristic facial features, a congenital heart defect, and mental retardation, has been defined by molecular analysis of DS patients with partial trisomy 21. The 2.5-Mb region around the marker D21S55 between D21S17 and ERG in 21q22 is thought to be important, although contributions of other regions cannot be excluded. In this region, we focused on a 1.6-Mb region between a NotI site, LA68 (D21S396, which is mapped distal to D21S17) and ERG, because analysis of a Japanese DS family with partial trisomy 21 revealed that the proximal border of its triplicated region was distal to LA68. We constructed P1 contigs with 46 P1 clones covering more than 95% of the 1.6-Mb region. A high-resolution restriction map using BamHI was also constructed for more details analysis. Our P1 contig map supplements other physical maps previously reported and provides useful materials for further analysis including isolation and sequencing of the DS region. 31 refs., 7 figs., 1 tab.

  16. Differential Epigenetic Effects of Atmospheric Cold Plasma on MCF-7 and MDA-MB-231 Breast Cancer Cells.

    Science.gov (United States)

    Park, Sung-Bin; Kim, Byungtak; Bae, Hansol; Lee, Hyunkyung; Lee, Seungyeon; Choi, Eun H; Kim, Sun Jung

    2015-01-01

    Cold atmospheric plasma (plasma) has emerged as a novel tool for a cancer treatment option, having been successfully applied to a few types of cancer cells, as well as tissues. However, to date, no studies have been performed to examine the effect of plasma on epigenetic alterations, including CpG methylation. In this study, the effects of plasma on DNA methylation changes in breast cancer cells were examined by treating cultured MCF-7 and MDA-MB-231 cells, representing estrogen-positive and estrogen-negative cancer cells, respectively, with plasma. A pyrosequencing analysis of Alu indicated that a specific CpG site was induced to be hypomethylated from 23.4 to 20.3% (p plasma treatment in the estrogen-negative MDA-MB-231 cells only. A genome-wide methylation analysis identified "cellular movement, connective tissue development and function, tissue development" and "cell-to-cell signaling and interaction, cell death and survival, cellular development" as the top networks. Of the two cell types, the MDA-MB-231 cells underwent a higher rate of apoptosis and a decreased proliferation rate upon plasma treatment. Taken together, these results indicate that plasma induces epigenetic and cellular changes in a cell type-specific manner, suggesting that a careful screening of target cells and tissues is necessary for the potential application of plasma as a cancer treatment option.

  17. Differential Epigenetic Effects of Atmospheric Cold Plasma on MCF-7 and MDA-MB-231 Breast Cancer Cells.

    Directory of Open Access Journals (Sweden)

    Sung-Bin Park

    Full Text Available Cold atmospheric plasma (plasma has emerged as a novel tool for a cancer treatment option, having been successfully applied to a few types of cancer cells, as well as tissues. However, to date, no studies have been performed to examine the effect of plasma on epigenetic alterations, including CpG methylation. In this study, the effects of plasma on DNA methylation changes in breast cancer cells were examined by treating cultured MCF-7 and MDA-MB-231 cells, representing estrogen-positive and estrogen-negative cancer cells, respectively, with plasma. A pyrosequencing analysis of Alu indicated that a specific CpG site was induced to be hypomethylated from 23.4 to 20.3% (p < 0.05 by plasma treatment in the estrogen-negative MDA-MB-231 cells only. A genome-wide methylation analysis identified "cellular movement, connective tissue development and function, tissue development" and "cell-to-cell signaling and interaction, cell death and survival, cellular development" as the top networks. Of the two cell types, the MDA-MB-231 cells underwent a higher rate of apoptosis and a decreased proliferation rate upon plasma treatment. Taken together, these results indicate that plasma induces epigenetic and cellular changes in a cell type-specific manner, suggesting that a careful screening of target cells and tissues is necessary for the potential application of plasma as a cancer treatment option.

  18. Cytotoxic effect of sanguiin H-6 on MCF-7 and MDA-MB-231 human breast carcinoma cells.

    Science.gov (United States)

    Park, Eun-Ji; Lee, Dahae; Baek, Seon-Eun; Kim, Ki Hyun; Kang, Ki Sung; Jang, Tae Su; Lee, Hye Lim; Song, Ji Hoon; Yoo, Jeong-Eun

    2017-09-15

    Sanguiin H-6 is a dimer of casuarictin linked by a bond between the gallic acid residue and one of the hexahydroxydiphenic acid units. It is an effective compound extracted from Rubus coreanus. It has an anticancer effect against several human cancer cells; however, its effect on breast cancer cells has not been clearly demonstrated. Thus, we aimed to investigate the anticancer effect and mechanism of action of sanguiin H-6 against two human breast carcinoma cell lines (MCF-7 and MDA-MB-231). We found that sanguiin H-6 significantly reduced cell viability in a concentration-dependent manner. It also increased the rates at which MCF-7 and MDA-MB-231 cells underwent apoptosis. Furthermore, sanguiin H-6 induced the cleavage of caspase-8, caspase-3, and poly(ADP-ribose) polymerase, which resulted in apoptosis. However, cleavage of caspase-9 was only detectable in MCF-7 cells. In addition, sanguiin H-6 increased the ratio of Bax to Bcl-2 in both MCF-7 and MDA-MB-231 cells. These findings suggest that sanguiin H-6 is a potent therapeutic agent against breast cancer cells. In addition, it exerts its anticancer effect in an estrogen-receptor-independent manner. Copyright © 2017 Elsevier Ltd. All rights reserved.

  19. Serum creatine kinase and CK-MB isoenzyme responses to acute and prolonged swimming in trained athletes.

    Science.gov (United States)

    Symanski, J D; McMurray, R G; Silverman, L M; Smith, B W; Siegel, A J

    1983-04-01

    Six highly-trained male swimmers completed a maximum work capacity tethered swim and a 1-h continuous tethered swim at approximately 70% VO2max in order to evaluate total serum creatine kinase and CK-MB isoenzyme changes. Venous blood obtained before, 5 min post-, 6 h post-, and 24 h post-exercise was analyzed for total serum CK (kinetic UV method, normal = less than 100 U/l) and CK-MB isoenzyme (quantitative electrophoretic technique, normal = less than 5 U/l). VO2max averaged 4.59 +/- 0.28 l/min, with a mean total work time of 24.5 min to achieve maximum capacity. Mean resting total CK was 100.5 +/- 15.8 U/l. Compared to rest, neither swim bout produced a significant (p greater than 0.05) elevation in mean total creatine kinase. No CK-MB isoenzyme was observed in any post-exercise blood sample. Swimming, performed by highly-trained swimmers at high levels of intensity or for prolonged durations, may not impose sufficient degrees of trauma producing muscular stress. Therefore, the structural integrity of the cell membrane is maintained and the loss of intracellular creatine kinase to the bloodstream prevented.

  20. Analysis of Protein–Protein Interactions in MCF-7 and MDA-MB-231 Cell Lines Using Phthalic Acid Chemical

    Directory of Open Access Journals (Sweden)

    Shih-Shin Liang

    2014-11-01

    Full Text Available Phthalates are a class of plasticizers that have been characterized as endocrine disrupters, and are associated with genital diseases, cardiotoxicity, hepatotoxicity, and nephrotoxicity in the GeneOntology gene/protein database. In this study, we synthesized phthalic acid chemical probes and demonstrated differing protein–protein interactions between MCF-7 cells and MDA-MB-231 breast cancer cell lines. Phthalic acid chemical probes were synthesized using silicon dioxide particle carriers, which were modified using the silanized linker 3-aminopropyl triethoxyslane (APTES. Incubation with cell lysates from breast cancer cell lines revealed interactions between phthalic acid and cellular proteins in MCF-7 and MDA-MB-231 cells. Subsequent proteomics analyses indicated 22 phthalic acid-binding proteins in both cell types, including heat shock cognate 71-kDa protein, ATP synthase subunit beta, and heat shock protein HSP 90-beta. In addition, 21 MCF-7-specific and 32 MDA-MB-231 specific phthalic acid-binding proteins were identified, including related proteasome proteins, heat shock 70-kDa protein, and NADPH dehydrogenase and ribosomal correlated proteins, ras-related proteins, and members of the heat shock protein family, respectively.