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Sample records for mads sig ager

  1. Mad Cow Disease

    ... Safe Videos for Educators Search English Español Mad Cow Disease KidsHealth / For Teens / Mad Cow Disease What's ... are people to get it? What Is Mad Cow Disease? Mad cow disease is an incurable, fatal ...

  2. Estudio comparativo entre SIG propietario y SIG libre

    Mesa Díaz, Juan Ramón

    2008-01-01

    Estudio comparativo entre SIG propietario y SIG libre, focalizado en los casos particulares de Geomedia Pro (SIG Propietario) y gvSIG (SIG Libre). En el estudio se procede a determinar cuáles son los aspectos destacables de un SIG, para poder evaluarlos, posteriormente, en los dos SIG objeto del estudio y obtener una ponderación definitoria de cada SIG. A continuación, algunos de los aspectos evaluados en cada SIG: interoperabilidad, conexión a bases de datos espaciales, aspectos económ...

  3. [Madness in Foucault: art and madness, madness and unreason].

    Providello, Guilherme Gonzaga Duarte; Yasui, Silvio

    2013-10-01

    After presenting the ideas on madness and its interface with art as expressed in the writings of Michel Foucault, Peter Pál Pelbart, and Gilles Deleuze, the article explores how these authors question the relationship between art and madness. It begins with the notion that madness does not tell the truth about art, and vice versa, but that there are links between both that must be delved into if we are to engage in deeper reflection on the topic. The text problematizes the statement that madness is the absence of an oeuvre and examines how this impacts the possibility of achieving an artistic oeuvre. It further problematizes the idea of madness as excluded language, that is, the idea that madness implies not only the exclusion of the body but also the disqualification of discourse.

  4. NetSig

    Horn, Heiko; Lawrence, Michael S; Chouinard, Candace R

    2018-01-01

    Methods that integrate molecular network information and tumor genome data could complement gene-based statistical tests to identify likely new cancer genes; but such approaches are challenging to validate at scale, and their predictive value remains unclear. We developed a robust statistic (Net......Sig) that integrates protein interaction networks with data from 4,742 tumor exomes. NetSig can accurately classify known driver genes in 60% of tested tumor types and predicts 62 new driver candidates. Using a quantitative experimental framework to determine in vivo tumorigenic potential in mice, we found that Net......Sig candidates induce tumors at rates that are comparable to those of known oncogenes and are ten-fold higher than those of random genes. By reanalyzing nine tumor-inducing NetSig candidates in 242 patients with oncogene-negative lung adenocarcinomas, we find that two (AKT2 and TFDP2) are significantly amplified...

  5. Mad Cow Disease (For Parents)

    ... Safe Videos for Educators Search English Español Mad Cow Disease KidsHealth / For Parents / Mad Cow Disease What's ... Is Being Done About It Print About Mad Cow Disease Mad cow disease has been in the ...

  6. Child Computer Interaction SIG

    Read, Janet; Hourcade, Juan Pablo; Markopoulos, Panos

    a mixture of facilitated creative thinking and a world café approach to bring the community together to tackle these two key challenges. The CCI SIG will be the natural meeting place for members of this community at CHI and will disseminate its discussion to the CCI and CHI communities through...... the production of visual and interactive materials at the CHI conference....

  7. Abstracts of SIG Sessions.

    Proceedings of the ASIS Annual Meeting, 1994

    1994-01-01

    Includes abstracts of 18 special interest group (SIG) sessions. Highlights include natural language processing, information science and terminology science, classification, knowledge-intensive information systems, information value and ownership issues, economics and theories of information science, information retrieval interfaces, fuzzy thinking…

  8. At studere sig selv

    Beedholm, Kirsten; Frederiksen, Kirsten

    1999-01-01

    Hvad ville der ske, hvis alle eskimoer begyndte at studere eskimologi på bekostning af at leve som en 'rigtig' eskimo? Det spørger forfatterne om i denne artikel. De underviser begge på sygeplejeskoler og finder det en tankevækkende tendens, at det i sygeplejerskernes grunduddannelse er faget sel......, der studeres frem for det, som faget retter sig imod ­ nemlig patienterne....

  9. Rundt om Mad Men

    Nielsen, Jakob Isak

    2011-01-01

    Artiklen gør rede for Mad Mens tilblivelse, dens populærkulturelle efterdønninger, multimediale forgreninger og værkæstetiske karakteristika. "Story Matters Here" lyder AMCs motto, men Mad Men tilbyder et bredspektret engagement, der går langt ud over at følge med i en vedkommende fortælling...

  10. Mad Cow Disease

    Mad Cow Disease, or bovine spongiform encephalopathy (BSE) is one of ... humoral immunity is developed against such infections. ... Most infecti ve agents, ranging from the more complex protozoans to bacteri(! and viruses, contain nucleic.

  11. MADS Users' Guide

    Moerder, Daniel D.

    2014-01-01

    MADS (Minimization Assistant for Dynamical Systems) is a trajectory optimization code in which a user-specified performance measure is directly minimized, subject to constraints placed on a low-order discretization of user-supplied plant ordinary differential equations. This document describes the mathematical formulation of the set of trajectory optimization problems for which MADS is suitable, and describes the user interface. Usage examples are provided.

  12. Thermodynamic assessments of the Ag-Er and Er-Y systems

    Wang, S.L.; Wang, C.P.; Liu, X.J.; Tang, A.T.; Pan, F.S.; Ishida, K.

    2010-01-01

    The phase diagrams and thermodynamic properties in the Ag-Er and Er-Y binary systems have been assessed by using the CALPHAD (Calculation of Phase Diagrams) method on the basis of the experimental data including the thermodynamic properties and phase equilibria. The Gibbs free energies of the liquid, bcc, fcc, and hcp phases were described by the subregular solution model with the Redlich-Kister equation, and those of intermetallic compounds (Ag 2 Er and AgEr phases) were treated as stoichiometric compounds, and Ag 51 Er 14 phase was modeled by the sublattice model in the Ag-Er binary system. The thermodynamic parameters of the Ag-Er and Er-Y binary systems were obtained, and an agreement between the calculated results and experimental data was obtained for each binary system.

  13. MAD Phasing with Krypton

    Cohen, A.

    2001-01-01

    Phasing of two proteins, the 17 kDa Fe protein myoglobin from sperm whale (P. catodon) and an 18 kDa protein (SP18) from green abalone (H. fulgens), using Kr-edge MAD with frozen crystals demonstrates the feasibility of this technique as a routine method for structure determination

  14. Ungdomslitteratur former(er) sig

    Henkel, Ayoe Qvist

    2016-01-01

    Igennem en 'mediesensitiv' analyse af romanen "Akavet" af Ronnie Andersen (2014) og perspektivering til andre aktuelle romaner for og med unge undersøger artiklen, hvordan ungdomslitteratur udvikler sig i dialog med digitale og mediebaserede impulser, og hvilke konsekvenser for udsigelserne om...... ungdomsliv og ungdomslitteraturens æstetik og mulige egenart, denne udvikling har. Artiklen baserer sig på en materialitetstilgang særligt inspireret af N. Katherine Hayles, som ikke tidligere har fået opmærksomhed i læsninger af ungdomslitteratur eller i diskussioner af ungdomslitteraturens mulige egenart......, at ungdomslitteratur drejer sig om unges udviklingsproces fra barndom og til voksenhed og dermed skildrer en overgang præget af linearitet, modning og vækst. Artiklen konkluderer, at der er ungdomslitteratur, der realiseres på andre præmisser, og Akavet kan ses som eksponent for en bevægelse fra ungdomslitteratur som...

  15. Baseline Analyses of SIG Applications and SIG-Eligible and SIG-Awarded Schools. NCEE 2011-4019

    Hurlburt, Steven; Le Floch, Kerstin Carlson; Therriault, Susan Bowles; Cole, Susan

    2011-01-01

    The Study of School Turnaround is an examination of the implementation of School Improvement Grants (SIG) authorized under Title I section 1003(g) of the "Elementary and Secondary Education Act" and supplemented by the "American Recovery and Reinvestment Act of 2009." "Baseline Analyses of SIG Applications and SIG-Eligible…

  16. MadEvent: automatic event generation with MadGraph

    Maltoni, Fabio; Stelzer, Tim

    2003-01-01

    We present a new multi-channel integration method and its implementation in the multi-purpose event generator MadEvent, which is based on MadGraph. Given a process, MadGraph automatically identifies all the relevant subprocesses, generates both the amplitudes and the mappings needed for an efficient integration over the phase space, and passes them to MadEvent. As a result, a process-specific, stand-alone code is produced that allows the user to calculate cross sections and produce unweighted events in a standard output format. Several examples are given for processes that are relevant for physics studies at present and forthcoming colliders. (author)

  17. Child-Computer Interaction SIG

    Hourcade, Juan Pablo; Revelle, Glenda; Zeising, Anja

    2016-01-01

    This SIG will provide child-computer interaction researchers and practitioners an opportunity to discuss four topics that represent new challenges and opportunities for the community. The four areas are: interactive technologies for children under the age of five, technology for inclusion, privacy...... and information security in the age of the quantified self, and the maker movement....

  18. Madness as disability.

    Gilman, Sander L

    2014-12-01

    How does society imagine mental illness? Does this shift radically over time and with different social attitudes as well as scientific discoveries about the origins and meanings of mental illness? What happens when we begin to think about mental illness as madness, as a malleable concept constantly shifting its meaning? We thus look at the meanings associated with 'general paralysis of the insane' in the nineteenth century and autism today in regard to disability. In this case study we examine the claims by scholars such as the anthropologist Emily Martin and the psychiatrist Kay Jamison as to the relationship between mental illness, disability and creativity. Today, the health sciences have become concerned with mental illness as a form of disability. How does this change the meaning of madness for practitioners and patients? © The Author(s) 2014.

  19. Madness in Shakespeare's Characters

    Nuno Borja-Santos

    2014-10-01

    Full Text Available This paper begins with an introduction where the aims are explained: a psychopathological analysis of a Shakespearean character - Othello – followed by the discussion of the English dramatist’s importance in helping us understand madness in the emergent world of Renaissance. The main characteristics of Othello’s personality, which allowed the development of his jealousy delusion, are described. Finally, the conclusions underline the overlap of the symptoms developed by the character with the DSM-IV classification.

  20. [Surrealism and madness].

    Flora, Κ

    2017-01-01

    This article attempts an approach of madness by surrealism, as reflected in the pathway of the surrealist movement. In the light of enlargement of the concept of mental illness and the experience of madness, an approach is being attempted regarding the early surrealist views as they precursory appear e.g. from the case of Hieronymus Bosch to the meeting of the dominant psychiatry and the surrealist movement in the 19th and 20th century. Then, the paper attempts to present the main positions of representatives of the movement, such as Breton, Dali and Kalas. These three surrealists were chosen among others, for this brief report, as the representatives of three remarkable moments in the surrealistic route. Breton introduces the element of fiction and hyper-reality while he questions the distinction between normal and abnormal element. Dali with his paranoid critical method reconciles actual representations with mythical and symbolic elements, breaking through the limits between objectivity and subjectivity. Kalas puts forward the social origin of insanity along with the fundamental surrealist notions of individual freedom and will. For a more complete understanding of this attempt, it was considered useful to include elements of the main views on madness from the standpoint of a critical approach in psychiatry and psychology. The surrealistic view seems to be close to this critical approach which is likely to have been affected by it on the level in which the movements and scientific fields meet and interact. The relationship between surrealism, the notion and expression of madness and the absurd seems to be inherent to the very development of the movement through its core and individual pursuits. In conclusion, the relationship between surrealism and the notion and expression of the madness and the absurd seems to be inherent to the very birth of the movement through its main positions and pursuits. The question of so-called reality, its overshoot and the vision of

  1. MAD parsing and conversion code

    Mokhov, Dmitri N.

    2000-01-01

    The authors describe design and implementation issues while developing an embeddable MAD language parser. Two working applications of the parser are also described, namely, MAD-> C++ converter and C++ factory. The report contains some relevant details about the parser and examples of converted code. It also describes some of the problems that were encountered and the solutions found for them

  2. MAD data collection - current trends

    Dementieva, I.; Evans, G.; Joachimiak, A.; Sanishvili, R.; Walsh, M. A.

    1999-01-01

    The multi-wavelength anomalous diffraction, or MAD, method of determining protein structure is becoming routine in protein crystallography. An increase in the number of tuneable synchrotrons beamlines coupled with the widespread availability position-sensitive X-ray detectors based on charged-coupled devices and having fast readout raised MAD structure determination to a new and exciting level. Ultra-fast MAD data collection is now possible. Recognition of the value of selenium for phasing protein structures and improvement of methods for incorporating selenium into proteins in the form of selenomethionine have attracted greater interest in the MAD method. Recent developments in crystallographic software are complimenting the above advances, paving the way for rapid protein structure determination. An overview of a typical MAD experiment is described here, with emphasis on the rates and quality of data acquisition now achievable at beamlines developed at third-generation synchrotrons sources

  3. Jamaah kraton: the Muslim new agers from Pekalongan

    Noor Aida

    2012-12-01

    Full Text Available The objective of this research was to know Muslim New Age identity, how theNew Age practitioners identify themselves as Muslim and apply religiosity, attitudeand expression in their personal and social life. This research discussesabout the religious experiences perceived by those practitioners of ShamballaMulti Dimension Healing.It was a qualitative research using analytical descriptive method, in which theresearcher tried to describe the Muslim New Agers identity by explaining theirritual and their perception about their religion and tradition. The location of thisresearch was in Pekalongan, because it is a good example to describe how NewAge impact to religion and tradition by describing how some Arabian MoslemPekalongan conducted some New Age activities.The research finding shows that Jamaah Kraton from Pekalongan feel comfortablewith New Age because of two main reasons: the fascinating teaching andthe charismatic leader. Moreover, they able to negotiate their religious identityproblem by passing over and mixed all religion’s teaching which has no contrarywith their main religion and tradition, Islam and Arab tradition. If they find anycontradiction (in common definition of other Muslim, they will not feel that theyconduct heresy, and the comfort ability they had picturing of a ‘merger and crossover’,‘a creation of a mixed cultures and’ and ‘syncretism’. Di Indonesia, terutama sejak awal 1990-an, kita menyaksikan penuhnya tempatkajian tasawuf dan maraknya buku-buku tentang ini. Bahkan fenomena bangkitnyaspiritualitas ini sudah pula memasuki kalangan profesional dan bisnis, berkatkehadiran KH Abdullah Gymnastiar (Aa Gym dengan Manajemen Qolbu, atau M.Arifin Ilham dengan gerakan zikir, atau Ary Ginandjar dengan pelatihan ESQ.Teknik reiki dan meditasi modern seperti Anand Asram dan Brahma Kumarisjuga digemari di kalangan kaum muda dan paruh baya, tidak terkecuali denganReiki Shamballa yang tergolong baru di Indonesia, juga

  4. [Travelers, mad, wandering].

    Vaschetto, Emilio

    2014-01-01

    This article explores the notion of "wandering" through the use of some phenomena enrolled at the dawn of modernity such as the Rousseau dromomanie's philosopher and writer, the origin of the first mad traveler (Albert Dadas), epidemics of mad travelers Europe and romantic tourism (with renewed acquires significance in the "beat generation" of the twentieth century). These historical facts are "mounting" as play contemporary manifestations such as loss, disorientation, to lose one's way, and wandering without reducing them only to clinical psychosis. Readings of classic psychiatrists such as Régis, Foville, Sérieux and Capgras, Tissié, go hand in hand with the current readings of the philosopher Ian Hacking and critics of pop culture as S. Reynolds and D. Diederichsen, illustrating how the travel's phenomenon can make different subjective configurations depending on historical times. In conclusion it is noted that not only psychosis exposes the wandering soul of suffering but there are also subject positions (as will be exemplified in a clinical case) and go no further nesting wandering into human existence.

  5. SigE Is a Chaperone for the Salmonella enterica Serovar Typhimurium Invasion Protein SigD

    Darwin, K. Heran; Robinson, Lloyd S.; Miller, Virginia L.

    2001-01-01

    SigD is translocated into eucaryotic cells by a type III secretion system. In this work, evidence that the putative chaperone SigE directly interacts with SigD is presented. A bacterial two-hybrid system demonstrated that SigE can interact with itself and SigD. In addition, SigD was specifically copurified with SigE-His6 on a nickel column.

  6. Når fremtiden tegner sig

    Nielsen, Mette Lykke; Katznelson, Noemi

    Når fremtiden tegner sig er en rapport om unge i et yderkantsområder i Danmark. Den giver indblik i nogle af de subjektive, kulturelle og samfundsmæssige mekanismer der kan få betydning for, hvordan fremtiden tegner sig for unge.......Når fremtiden tegner sig er en rapport om unge i et yderkantsområder i Danmark. Den giver indblik i nogle af de subjektive, kulturelle og samfundsmæssige mekanismer der kan få betydning for, hvordan fremtiden tegner sig for unge....

  7. MadGraph/MadEvent. The new web generation

    Alwall, J.

    2007-01-01

    The new web-based version of the automatized process and event generator MadGraph/MadEvent is now available. Recent developments are: New models, notably MSSM, 2HDM and a framework for addition of user-defined models, inclusive sample generation and on-line hadronization and detector simulation. Event generation can be done on-line on any of our clusters. (author)

  8. A direct role of Mad1 in the spindle assembly checkpoint beyond Mad2 kinetochore recruitment

    Kruse, Thomas; Larsen, Marie Sofie Yoo; Sedgwick, Garry G

    2014-01-01

    in the SAC beyond recruitment of C-Mad2 to kinetochores has not yet been addressed. Here, we show that Mad1 is required for mitotic arrest even when C-Mad2 is artificially recruited to kinetochores, indicating that it has indeed an additional function in promoting the checkpoint. The C-terminal globular...... domain of Mad1 and conserved residues in this region are required for this unexpected function of Mad1........ The conversion of O-Mad2 into C-Mad2 at unattached kinetochores is thought to be a key step in activating the SAC. The "template model" proposes that this is achieved by the recruitment of soluble O-Mad2 to C-Mad2 bound at kinetochores through its interaction with Mad1. Whether Mad1 has additional roles...

  9. Alternative Sigma Factors SigF, SigE, and SigG Are Essential for Sporulation in Clostridium botulinum ATCC 3502

    Kirk, David G.; Zhang, Zhen; Korkeala, Hannu; Lindström, Miia

    2014-01-01

    Clostridium botulinum produces heat-resistant endospores that may germinate and outgrow into neurotoxic cultures in foods. Sporulation is regulated by the transcription factor Spo0A and the alternative sigma factors SigF, SigE, SigG, and SigK in most spore formers studied to date. We constructed mutants of sigF, sigE, and sigG in C. botulinum ATCC 3502 and used quantitative reverse transcriptase PCR and electron microscopy to assess their expression of the sporulation pathway on transcription...

  10. Mad Honey Disease

    Laurentiu Broscaru

    2017-11-01

    Full Text Available A 46-years old woman presented with acute onset of nausea, vomiting and prostration in the ER. She appeared ill and was poorly responsive to verbal stimuli. The physical examination showed a systolic blood pressure of 60 mmHg and a pulse of 40 bpm. ECG was notable for slight ST-elevations in the inferior leads. Right ventricular myocardial infarction with cardiogenic shock and bradycardia was suspected. Supportive therapy with catecholamines was initiated and a emergency coronary angiography was arranged. However, lab results showed normal troponin levels and a subsequent echocardiogram showed the absence of abnormal wall motions. By thorough history taking with the spouse it turned out that the patient had consumed a Turkish honey approximately an hour before the beginning of the symptoms. The patient made a full recovery within 24 hours with only supportive therapy. In retrospect the clinical presentation was highly indicative of poisoning with Grayanotoxins from a plant, Rhododendron, which is found as contaminant in some sorts of honey in the Black Sea area. A pollen analysis confirmed the presence of Rhododendron in a honey sample.  Historically this poisoning is mentioned over the millennia as mad honey disease. The ST-elevations in the ECG were a sign of early repolarization, a non-pathological finding.

  11. BioSig - An application of Octave

    Schlögl, Alois

    2006-01-01

    BioSig is an open source software library for biomedical signal processing. Most users in the field are using Matlab; however, significant effort was undertaken to provide compatibility to Octave, too. This effort has been widely successful, only some non-critical components relying on a graphical user interface are missing. Now, installing BioSig on Octave is as easy as on Matlab. Moreover, a benchmark test based on BioSig has been developed and the benchmark results of several platforms are...

  12. Madness in Sartre's "The Room"

    Jongeneel, E.C.S.

    2009-01-01

    In "The Room," part of his short story collection, The Wall (1938), Jean-Paul Sartre investigates madness as an alternative way of bourgeois life and thus takes a stand in the contemporary debate on the existential status of mental illness. "The Room" is a case-study of a "limit situation," as well

  13. Roles of SigB and SigF in the Mycobacterium tuberculosis Sigma Factor Network▿ †

    Lee, Jong-Hee; Karakousis, Petros C.; Bishai, William R.

    2007-01-01

    To characterize the roles of SigB and SigF in sigma factor regulation in Mycobacterium tuberculosis, we used chemically inducible recombinant strains to conditionally overexpress sigB and sigF. Using whole genomic microarray analysis and quantitative reverse transcription-PCR, we investigated the resulting global transcriptional changes after sigB induction, and we specifically tested the relative expression of other sigma factor genes after knock-in expression of sigB and sigF. Overexpressio...

  14. [Genome similarity of Baikal omul and sig].

    Bychenko, O S; Sukhanova, L V; Ukolova, S S; Skvortsov, T A; Potapov, V K; Azhikina, T L; Sverdlov, E D

    2009-01-01

    Two members of the Baikal sig family, a lake sig (Coregonus lavaretus baicalensis Dybovsky) and omul (C. autumnalis migratorius Georgi), are close relatives that diverged from the same ancestor 10-20 thousand years ago. In this work, we studied genomic polymorphism of these two fish species. The method of subtraction hybridization (SH) did not reveal the presence of extended sequences in the sig genome and their absence in the omul genome. All the fragments found by SH corresponded to polymorphous noncoding genome regions varying in mononucleotide substitutions and short deletions. Many of them are mapped close to genes of the immune system and have regions identical to the Tc-1-like transposons abundant among fish, whose transcription activity may affect the expression of adjacent genes. Thus, we showed for the first time that genetic differences between Baikal sig family members are extremely small and cannot be revealed by the SH method. This is another endorsement of the hypothesis on the close relationship between Baikal sig and omul and their evolutionarily recent divergence from a common ancestor.

  15. SigWinR; the SigWin-detector updated and ported to R.

    de Leeuw, Wim C; Rauwerda, Han; Inda, Márcia A; Bruning, Oskar; Breit, Timo M

    2009-10-06

    Our SigWin-detector discovers significantly enriched windows of (genomic) elements in any sequence of values (genes or other genomic elements in a DNA sequence) in a fast and reproducible way. However, since it is grid based, only (life) scientists with access to the grid can use this tool. Therefore and on request, we have developed the SigWinR package which makes the SigWin-detector available to a much wider audience. At the same time, we have introduced several improvements to its algorithm as well as its functionality, based on the feedback of SigWin-detector end users. To allow usage of the SigWin-detector on a desktop computer, we have rewritten it as a package for R: SigWinR. R is a free and widely used multi platform software environment for statistical computing and graphics. The package can be installed and used on all platforms for which R is available. The improvements involve: a visualization of the input-sequence values supporting the interpretation of Ridgeograms; a visualization allowing for an easy interpretation of enriched or depleted regions in the sequence using windows of pre-defined size; an option that allows the analysis of circular sequences, which results in rectangular Ridgeograms; an application to identify regions of co-altered gene expression (ROCAGEs) with a real-life biological use-case; adaptation of the algorithm to allow analysis of non-regularly sampled data using a constant window size in physical space without resampling the data. To achieve this, support for analysis of windows with an even number of elements was added. By porting the SigWin-detector as an R package, SigWinR, improving its algorithm and functionality combined with adequate performance, we have made SigWin-detector more useful as well as more easily accessible to scientists without a grid infrastructure.

  16. SigWinR; the SigWin-detector updated and ported to R

    Breit Timo M

    2009-10-01

    Full Text Available Abstract Background Our SigWin-detector discovers significantly enriched windows of (genomic elements in any sequence of values (genes or other genomic elements in a DNA sequence in a fast and reproducible way. However, since it is grid based, only (life scientists with access to the grid can use this tool. Therefore and on request, we have developed the SigWinR package which makes the SigWin-detector available to a much wider audience. At the same time, we have introduced several improvements to its algorithm as well as its functionality, based on the feedback of SigWin-detector end users. Findings To allow usage of the SigWin-detector on a desktop computer, we have rewritten it as a package for R: SigWinR. R is a free and widely used multi platform software environment for statistical computing and graphics. The package can be installed and used on all platforms for which R is available. The improvements involve: a visualization of the input-sequence values supporting the interpretation of Ridgeograms; a visualization allowing for an easy interpretation of enriched or depleted regions in the sequence using windows of pre-defined size; an option that allows the analysis of circular sequences, which results in rectangular Ridgeograms; an application to identify regions of co-altered gene expression (ROCAGEs with a real-life biological use-case; adaptation of the algorithm to allow analysis of non-regularly sampled data using a constant window size in physical space without resampling the data. To achieve this, support for analysis of windows with an even number of elements was added. Conclusion By porting the SigWin-detector as an R package, SigWinR, improving its algorithm and functionality combined with adequate performance, we have made SigWin-detector more useful as well as more easily accessible to scientists without a grid infrastructure.

  17. Abject Magic: Reasoning Madness in Justine Larbalestier's "Magic or Madness" Trilogy

    Potter, Troy

    2013-01-01

    This paper explores the representation of magic and madness in Justine Larbalestier's "Magic or Madness" trilogy (2005-2007). Throughout the series, magic is constructed as an abject and disabling force that threatens to disable magic-wielders, either through madness or death. Despite being represented as a ubiquitous force, the…

  18. MADS-box gene evolution - structure and transcription patterns

    Johansen, Bo; Pedersen, Louise Buchholt; Skipper, Martin

    2002-01-01

    Mads-box genes, ABC model, Evolution, Phylogeny, Transcription patterns, Gene structure, Conserved motifs......Mads-box genes, ABC model, Evolution, Phylogeny, Transcription patterns, Gene structure, Conserved motifs...

  19. SIG aplicado ao Ensino de Geografia

    Dornelles, Liane Maria Azevedo; Universidade do Estado do Rio de Janeiro - UERJ

    2009-01-01

    Este trabalho descreve as atividades desenvolvidas junto à disciplina eletiva SIG aplicado ao ensino de Geografia. Foram elaboradas aplicações ambientais para os Ensinos Fundamental e Médio, com auxílio dos programas VistaSAGA/UFRJ, SISPLAMTE 5as com GIS, SPRING/INPE e MapServer.

  20. Critical Role of a Single Position in the −35 Element for Promoter Recognition by Mycobacterium tuberculosis SigE and SigH▿

    Song, Taeksun; Song, Seung-Eun; Raman, Sahadevan; Anaya, Mauricio; Husson, Robert N.

    2008-01-01

    Mycobacterial SigE and SigH both initiate transcription from the sigB promoter, suggesting that they recognize similar sequences. Through mutational and primer extension analyses, we determined that SigE and SigH recognize nearly identical promoters, with differences at the 3′ end of the −35 element distinguishing between SigE- and SigH-dependent promoters.

  1. Desarrollo de un SIG para dispositivos móviles utilizando gvSIG Mobile

    Pérez Álvarez, Francisco

    2012-01-01

    Este proyecto se inicia con la introducción al mundo de los SIG basados en software de código abierto, como es el caso del gvSIG Mobile y gvSIG Desktop. Basándonos en estos dos programas, hemos creado una aplicación SIG para dispositivos móviles (PDA’s y smartphones) gracias a la cual, será posible actualizar cartografía en tiempo real directamente en campo. Para introducirse de pleno en el tema del proyecto, se realizó un análisis detallado sobre las necesidades que podrían existir a l...

  2. Fabrication of Gamma Detectors Based on Magnetic Ag:Er Microcalorimeters

    Friedrich, Stephan [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Boyd, Stephen [Univ. of New Mexico, Albuquerque, NM (United States); Cantor, Robin [STAR Cryoelectronics, Santa Fe, NM (United States)

    2015-11-25

    This report discusses the photolithographic fabrication of ultra-high resolution gamma-ray detectors based on magnetic microcalorimeters (MMCs). The MMC uses a novel Er-doped silver sensor (Ag:Er) that is expected to have higher sensitivity than the Er-doped gold (Au:Er) sensors currently in use. The MMC also integrates the first-stage SQUID preamplifier on the same chip as the MMC gamma detector to increase its signal-to-noise ratio. In addition, the MMC uses a passive Ta-Nb heat switch to replace one of the common long-term failure points in earlier detectors. This report discusses the fabrication process we have developed to implement the proposed improvements.

  3. Fabrication of Gamma Detectors Based on Magnetic Ag:Er Microcalorimeters

    Friedrich, Stephan [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Boyd, Stephen [Univ. of New Mexico, Albuquerque, NM (United States); Cantor, Robin [STAR Cryoelectronics, Santa Fe, NM (United States)

    2016-05-06

    This report discusses the photolithographic fabrication of ultra-high resolution gamma-ray detectors based on magnetic microcalorimeters (MMCs). The MMC uses a novel Er-doped silver sensor (Ag:Er) that is expected to have higher sensitivity than the Er-doped gold (Au:Er) sensors currently in use. The MMC also integrates the first-stage SQUID preamplifier on the same chip as the MMC gamma detector to increase its signal-to-noise ratio. In addition, the MMC uses a passive Ta-Nb heat switch to replace one of the common long-term failure points in earlier detectors. This report discusses the fabrication process we have developed to implement the proposed improvements.

  4. Fabrication of Gamma Detectors Based on Magnetic Ag:Er Microcalorimeters

    Friedrich, Stephan; Boyd, Stephen; Cantor, Robin

    2016-01-01

    This report discusses the photolithographic fabrication of ultra-high resolution gamma-ray detectors based on magnetic microcalorimeters (MMCs). The MMC uses a novel Er-doped silver sensor (Ag:Er) that is expected to have higher sensitivity than the Er-doped gold (Au:Er) sensors currently in use. The MMC also integrates the first-stage SQUID preamplifier on the same chip as the MMC gamma detector to increase its signal-to-noise ratio. In addition, the MMC uses a passive Ta-Nb heat switch to replace one of the common long-term failure points in earlier detectors. This report discusses the fabrication process we have developed to implement the proposed improvements.

  5. Fabrication of Gamma Detectors Based on Magnetic Ag:Er Microcalorimeters

    Friedrich, Stephan; Boyd, Stephen; Cantor, Robin

    2015-01-01

    This report discusses the photolithographic fabrication of ultra-high resolution gamma-ray detectors based on magnetic microcalorimeters (MMCs). The MMC uses a novel Er-doped silver sensor (Ag:Er) that is expected to have higher sensitivity than the Er-doped gold (Au:Er) sensors currently in use. The MMC also integrates the first-stage SQUID preamplifier on the same chip as the MMC gamma detector to increase its signal-to-noise ratio. In addition, the MMC uses a passive Ta-Nb heat switch to replace one of the common long-term failure points in earlier detectors. This report discusses the fabrication process we have developed to implement the proposed improvements.

  6. Elizabethan madness: on London's stage.

    Dalby, J T

    1997-12-01

    During the reign of Elizabeth I (1558-1603) a renaissance of both literary and political history occurred. The stage was transformed from primitive echoes of the morality plays to a vibrant and diverse exploration of human endeavor and man's place in the universe. The titanic literary figure of Shakespeare today veils a group of friends and challengers whose pens strove for the same goal. The depiction of madness was ubiquitous during plays of this time and reflection on the views of this group of men gives us a more reliable insight into mental illness then and today.

  7. Madness Decolonized?: Madness as Transnational Identity in Gail Hornstein's Agnes's Jacket.

    Miller, Gavin

    2017-02-13

    The US psychologist Gail Hornstein's monograph, Agnes's Jacket: A Psychologist's Search for the Meanings of Madness (2009), is an important intervention in the identity politics of the mad movement. Hornstein offers a resignified vision of mad identity that embroiders the central trope of an "anti-colonial" struggle to reclaim the experiential world "colonized" by psychiatry. A series of literal and figurative appeals makes recourse to the inner world and (corresponding) cultural world of the mad as well as to the ethno-symbolic cultural materials of dormant nationhood. This rhetoric is augmented by a model in which the mad comprise a diaspora without an origin, coalescing into a single transnational community. The mad are also depicted as persons displaced from their metaphorical homeland, the "inner" world "colonized" by the psychiatric regime. There are a number of difficulties with Hornstein's rhetoric, however. Her "ethnicity-and-rights" response to the oppression of the mad is symptomatic of Western parochialism, while her proposed transmutation of putative psychopathology from limit upon identity to parameter of successful identity is open to contestation. Moreover, unless one accepts Hornstein's porous vision of mad identity, her self-ascribed insider status in relation to the mad community may present a problematic "re-colonization" of mad experience.

  8. Mand falder - og rejser sig igen

    Evron, Lotte Orr

    2016-01-01

    Mand falder - og rejser sig igen Anmeldelse af Lotte Evron 6 stjerner Gennem et år følger Anne Wivel med kamera og samtaler sin mangeårige ven, Per Kirkebys, vej mod en ny hverdag efter et fald. Med venskabet som ramme opbygges et usædvanligt hudløst og respektfuldt portræt af Per Kirkeby – både...

  9. Relationship with BSE (Mad Cow Disease)

    ... Disease (CWD) Prion Diseases Relationship with BSE (Mad Cow Disease) Evidence Recommend on Facebook Tweet Share Compartir ... macaque monkeys inoculated with brain tissue obtained from cattle with BSE had clinical and neuropathological features strikingly ...

  10. Turned Back: Mad Men as Intermedial Melodrama

    Monique Rooney

    2012-09-01

    Full Text Available This essay draws on definitions of gesture (Giorgio Agamben and Peter Brooks and catachresis (Peter Brooks, Jacques Derrida to examine the primacy of non-verbal signifiers as communicators of meaning in AMC’s Mad Men. Beginning with an analysis of Mad Men’s credit sequence, it draws attention to Mad Men’s use of gesture and catachresis in relation to melodrama’s privileging of non-verbal and naturalistic expression and its persistence as an intermedial mode that has moved back and forth between various media (theatre, novel, cinema, television and now digital formats. It argues that Mad Men’s melodramatic aesthetic is one that obliquely, and via a gestural and rhetorical ‘turned back’, communicates its relation to the past and the present.

  11. A Network SIG is born, DECUS (Switzerland) Newsletter, May 1990

    Heagerty, Denise

    1990-01-01

    This article announces the formation of a Swiss DECUS (DEC Users Group) Network SIG in May 1990. The goal of this SIG is to help Swiss DECnet managers to plan transition from their proprietary DECnet Phase IV networks (e.g. the HEP/SPAN DECnet) to open networks based on DECnet Phase V/OSI. The SIG also proposes to address integration with UNIX based workstations using the Internet's TCP/IP protocols.

  12. MAD-X Training Course – 2016

    2016-01-01

    MAD-X 2016 is a annual course series at CERN, within the framework of the 2016 Technical Training Programme on the MAD-X tool used around the world for designing, studying and simulating beam physics for particle accelerators. The lecturer is Laurent Deniau from BE-APB, who has led the MAD team since 2011.    Two courses are available: Methodical Accelerator Design MAD-X: Beginners Session: 1-2 March (half day: mornings)   Methodical Accelerator Design MAD-X: Intermediate Session: 10-11 March (half day: mornings) Target audience: Designed for those needing to become familiar with and acquire some practical experience of particle accelerator design with MAD-X. Pre-requirements: The course requires some prior knowledge of accelerators and beam physics (e.g. optics) as the theory is not detailed. The series will be composed of 4 half-day lectures, given in English with questions and answers also possible in French. Participation in all lectures ...

  13. EduSIG: gvSIG aplicado a la enseñanza de la geografía

    Bermejo Domínguez, Juan A.; Anguix Alfaro, Álvaro; Juncos, Raúl

    2009-01-01

    EduSIG parte de la idea de disponer de un SIG como herramienta educativa para el aprendizaje de la geografía. Por un lado EduSIG consiste en un gvSIG más simple, sin herramientas complejas o muy técnicas, que permite navegar, consultar, construir y entender los mapas sin necesitar ninguna formación en Sistemas de Información Geográfica. Por otro lado pretende dar una visión didáctica de la enseñanza de la geografía, incluyendo tanto vistas temáticas predefinidas y diverso...

  14. Sig, sig selv> og KorpusDK - hvorfor det er svært både at skælde sig ud og at skille sig selv ud

    Ehlers, Katrine Rosendal; Vikner, Sten

    2017-01-01

    Denne artikel vil undersøge det danske refleksivsystem, baseret på den antagelse at der er to forskellige typer betingelser angående koreference som hver af de fire refleksive/non-refleksive pronominaltyper i dansk, dvs. sig/sig selv/hende/hende selv skal opfylde (jf. Vikner 1985). En type beting...

  15. Hvorfor sætter folk ild til sig selv?

    Harrebye, Silas

    2011-01-01

    Mellemøsten brød i brand efter en mand satte ild til sig selv. Et bevis på revolutioner skal selvantændes?......Mellemøsten brød i brand efter en mand satte ild til sig selv. Et bevis på revolutioner skal selvantændes?...

  16. KERNEL MAD ALGORITHM FOR RELATIVE RADIOMETRIC NORMALIZATION

    Y. Bai

    2016-06-01

    Full Text Available The multivariate alteration detection (MAD algorithm is commonly used in relative radiometric normalization. This algorithm is based on linear canonical correlation analysis (CCA which can analyze only linear relationships among bands. Therefore, we first introduce a new version of MAD in this study based on the established method known as kernel canonical correlation analysis (KCCA. The proposed method effectively extracts the non-linear and complex relationships among variables. We then conduct relative radiometric normalization experiments on both the linear CCA and KCCA version of the MAD algorithm with the use of Landsat-8 data of Beijing, China, and Gaofen-1(GF-1 data derived from South China. Finally, we analyze the difference between the two methods. Results show that the KCCA-based MAD can be satisfactorily applied to relative radiometric normalization, this algorithm can well describe the nonlinear relationship between multi-temporal images. This work is the first attempt to apply a KCCA-based MAD algorithm to relative radiometric normalization.

  17. Low-dose irradiation to head, neck, or chest during infancy as a possible cause of thyroid carcinoma in teen-agers

    Yoshida, Akira; Fukuda, Katsuhiro; Noguchi, Shiro; Hirohata, Tomio.

    1987-01-01

    A matched case-control study was performed to identify the etiologic factors for thyroid carcinoma in teen-agers. Twenty-seven cases and 69 controls were investigated to assess the significance of various maternal and subject factors. Irradiation during infancy was the only factor which showed a statistically significant association with the incidence of thyroid cancer in teen-agers (summary χ 2 = 8.040; d.f. = 1; P < 0.005). The estimated dose ranged from 0.2 to 40 rads on the head, neck, or chest during infancy. (author)

  18. Exit Planning at Anette's Mad APS

    Nellemann, Camilla

    2017-01-01

    This is a Danish version. The 60-year old Anette Hansen established her catering firm Anette's Mad in 2012. She wants to sell her company within 7 years but so far hasn't thought a lot about ownership transfer. Anette's Mad employs 5 people and has a yearly turnover of about DKK200,000. The company...... benefits from Anette's strong, local network. At this point, Anette has invested DKK1.5 million in her business which she hopes to get back once she sells the company. The question is how Anette can prepare her business for sale so that it may sell at the price that she aims at....

  19. Mad2 binding to Mad1 and Cdc20, rather than oligomerization, is required for the spindle checkpoint

    Sironi, L; Melixetian, M; Faretta, M

    2001-01-01

    Mad2 is a key component of the spindle checkpoint, a device that controls the fidelity of chromosome segregation in mitosis. The ability of Mad2 to form oligomers in vitro has been correlated with its ability to block the cell cycle upon injection into Xenopus embryos. Here we show that Mad2 forms...

  20. Cultural Mediators Seduced by Mad Men:

    Kristensen, Nete Nørgaard; Hellman, Heikki; Riegert, Kristina

    2017-01-01

    Based on theories about the role of cultural mediators in cultural production and using the TV series Mad Men as a case, this article investigates how cultural journalists in the Nordic countries have contributed to legitimizing “quality TV series” as a worthy field of aesthetic consumption. Key...

  1. Trans meets cis in MADS science

    Folter, de S.; Angenent, G.C.

    2006-01-01

    The interaction between a transcription factor and its binding site at the DNA is an integral part of transcriptional regulatory networks, which is fundamental for an understanding of biological processes. An example is the family of MADS domain transcription factors, which represent key regulators

  2. MADS goes genomic in conifers: towards determining the ancestral set of MADS-box genes in seed plants.

    Gramzow, Lydia; Weilandt, Lisa; Theißen, Günter

    2014-11-01

    MADS-box genes comprise a gene family coding for transcription factors. This gene family expanded greatly during land plant evolution such that the number of MADS-box genes ranges from one or two in green algae to around 100 in angiosperms. Given the crucial functions of MADS-box genes for nearly all aspects of plant development, the expansion of this gene family probably contributed to the increasing complexity of plants. However, the expansion of MADS-box genes during one important step of land plant evolution, namely the origin of seed plants, remains poorly understood due to the previous lack of whole-genome data for gymnosperms. The newly available genome sequences of Picea abies, Picea glauca and Pinus taeda were used to identify the complete set of MADS-box genes in these conifers. In addition, MADS-box genes were identified in the growing number of transcriptomes available for gymnosperms. With these datasets, phylogenies were constructed to determine the ancestral set of MADS-box genes of seed plants and to infer the ancestral functions of these genes. Type I MADS-box genes are under-represented in gymnosperms and only a minimum of two Type I MADS-box genes have been present in the most recent common ancestor (MRCA) of seed plants. In contrast, a large number of Type II MADS-box genes were found in gymnosperms. The MRCA of extant seed plants probably possessed at least 11-14 Type II MADS-box genes. In gymnosperms two duplications of Type II MADS-box genes were found, such that the MRCA of extant gymnosperms had at least 14-16 Type II MADS-box genes. The implied ancestral set of MADS-box genes for seed plants shows simplicity for Type I MADS-box genes and remarkable complexity for Type II MADS-box genes in terms of phylogeny and putative functions. The analysis of transcriptome data reveals that gymnosperm MADS-box genes are expressed in a great variety of tissues, indicating diverse roles of MADS-box genes for the development of gymnosperms. This study is

  3. Biostratigraphy, palaeoecology and palaeogeography of the mainly marine Ager Formation (Upper Paleocene — Lower Eocene) in the Tremp Basin, Central-South Pyrenees, Spain

    Gaemers, Pieter A.M.

    1978-01-01

    During the greater part of the Palaeogene the Tremp Basin was an area which underwent rapid subsidence as compared with the axial zone of the Pyrenees to the north, and the Ebro Massif to the south. As a result the sea occupied this area for a long time and deposition of the Ager Formation took

  4. Approche SIG pour une analyse spatiale des infrastructures ...

    SARAH

    31 janv. 2014 ... SIG jouent un rôle primordial dans l'implantation, le suivi et la gestion des infrastructures hydrauliques. L'utilisation de ces outils peut atténuer les difficultés d'approvisionnement en eau. Mots clés : SIG, distribution spatiale, infrastructure hydraulique, Zè, Bénin. ... accès à un dispositif d'assainissement de.

  5. … vandt sig Danmark al … - hvad mente Harald?

    Roesdahl, Else

    2013-01-01

    Diskussion af meningen med sætningen 'vandt sig Danmark al' på den store Jellingsten. Ordet 'vandt' tolkes i retning af nudansk 'vandt' og ikke som 'samlede', hvilket tit fremføres.......Diskussion af meningen med sætningen 'vandt sig Danmark al' på den store Jellingsten. Ordet 'vandt' tolkes i retning af nudansk 'vandt' og ikke som 'samlede', hvilket tit fremføres....

  6. Rediscovering Sig Socransky, the genius and his legacy.

    Teles, R P; Teles, F R F; Loesche, W J; Listgarten, M; Fine, D; Lindhe, J; Malament, K; Haffajee, A D

    2012-05-01

    Some individuals make contributions so vital to their field of knowledge that their names become almost synonymous with that field. This is the case of Sig Socransky and the field of periodontal microbiology. Sig Socransky, or simply Sig, was born in Toronto, Canada and received his DDS degree from the University of Toronto in 1957. He studied microbiology and periodontology at Harvard, receiving a certificate in 1961. That same year he was recruited to work as a Research Associate at the Forsyth Dental Center. In 1968, he was nominated Senior Member of the Staff and Head of the Department of Periodontology. During his 50-year career at Forsyth, Sig published over 300 manuscripts, keeping an average of 7 publications per year. His work had an indelible impact in the fields of periodontology and oral microbiology. All these accomplishments pale in comparison with the impact that Sig had on a personal level. We have collected testimonials from some of his former students, closest collaborators, and friends in an attempt to give readers an insight into Sig's personality. We hope we can offer those who knew him through his work a glimpse of how it felt to interact with this remarkable individual.

  7. The pineapple AcMADS1 promoter confers high level expression in tomato and arabidopsis flowering and fruiting tissues, but AcMADS1 does not complement the tomato LeMADS-RIN (rin) mutant

    A previous EST study identified a MADS box transcription factor coding sequence, AcMADS1, that is strongly induced during non-climacteric pineapple fruit ripening. Phylogenetic analyses place the AcMADS1 protein in the same superclade as LeMADS-RIN, a master regulator of fruit ripening upstream of e...

  8. Cloning and Functional Analysis of the MADS-box CiMADS9 Gene from Carya illinoinensis

    Zhang Jiyu

    2015-07-01

    Full Text Available A MADS-box gene, CiMADS9, was cloned from the male flowers of Carya illinoinensis by rapid amplification of cDNA ends. The gene was 1 077 bp with a 768 bp open reading frame encoding 255 amino acids. Multiple sequence comparisons revealed that CiMADS9 is a typical MIKC-type MADS-box gene with a MADS-box domain and a K semi-conserved region. Phylogenetic analysis indicated that CiMADS9 belongs to the AGL15 group of the MADS-box gene family. Quantitative reverse transcription polymerase chain reaction analysis indicated that the expression levels in reproductive organs (i.e., flowers and young fruits were considerably higher than in vegetative tissues (i.e., leaves and branches. The highest expression levels were observed in male flowers. An overexpression vector for CiMADS9 was constructed and the gene was inserted into the Arabidopsis thaliana genome. CiMADS9 expression was confirmed in all transgenic lines. Compared with wild-type plants, transgenic A. thaliana plants overexpressing CiMADS9 exhibited delayed flowering and an increased number of leaves.

  9. MAD-X progress and future plans

    Deniau, L

    2012-01-01

    The design efforts for the High Luminosity upgrade of the Large Hadron Collider (HL-LHC) will require significant extensions of the MAD-X code widely used for designing and simulating particles accelerators. These changes are framed into a global redesign of the MADX architecture meant to consolidate its structure, increase its robustness and flexibility, and improve its performance. Some examples of recent extensions to MAD-X like the RF-Multipole element will be presented. Improvement for models and algorithms selection providing better consistency of the results and a wider range of use will be discussed. The computation efficiency will also be addressed to profit better of modern technologies. In this paper, we will describe the last improvements and the future plans of the project.

  10. California and Irony in Mad Men

    Taveira, Rodney

    2012-01-01

    The combination of melodramatic and art cinematic techniques and influences in AMC’s television series Mad Men (2007¬–) reveals how a melodramatic televisuality can image novel modes of social and intimate relations and an alternative to the archetypal American narrative of the self-made man. Set in 1960s’ America, the series uses a contemporaneous and cosmopolitan California to triangulate the formal and narrative insistence of the past on the present. This triangulation is played out by Don...

  11. Automated MAD and MIR structure solution

    Terwilliger, Thomas C.; Berendzen, Joel

    1999-01-01

    A fully automated procedure for solving MIR and MAD structures has been developed using a scoring scheme to convert the structure-solution process into an optimization problem. Obtaining an electron-density map from X-ray diffraction data can be difficult and time-consuming even after the data have been collected, largely because MIR and MAD structure determinations currently require many subjective evaluations of the qualities of trial heavy-atom partial structures before a correct heavy-atom solution is obtained. A set of criteria for evaluating the quality of heavy-atom partial solutions in macromolecular crystallography have been developed. These have allowed the conversion of the crystal structure-solution process into an optimization problem and have allowed its automation. The SOLVE software has been used to solve MAD data sets with as many as 52 selenium sites in the asymmetric unit. The automated structure-solution process developed is a major step towards the fully automated structure-determination, model-building and refinement procedure which is needed for genomic scale structure determinations

  12. A case of acute hepatitis following mad honey ingestion

    Fatma Sari Dogan

    2015-12-01

    Full Text Available Acute hepatitis is characterized by liver inflammation and liver cell necrosis. The most frequently observed underlying cause thereof is viruses, but various other causes, such as alcohol, medication, or toxins may also lead thereto.In this paper, a case of acute hepatitis presenting with bradycardia, hypotension, and a prominent increase in liver enzymes following mad honey ingestion is discussed. Since there are only few cases of acute hepatitis following mad honey ingestion in the literature, we want to present this subject matter. Keywords: Mad honey poisoning, Mad honey intoxication, Bradycardia, Hypotension, Acute hepatitis

  13. Kan migration føre noget godt med sig?

    Pærregaard, Karsten

    2008-01-01

    Som regel har den globale migration rod i økonomisk ulighed, social uretfærdighed eller politisk forfølgelse i de lande, hvor migranterne kommer fra og for mange migranter. Mange år efter, at disse har fundet sig til rette og tilpasset sig de samfund, som har migreret til, fortsætter deres families...... hjemlandsbyer i Peru. Artiklen er baseret på et antropologisk forskningsprojekt, som jeg gennemførte fra 2003-2006 blandt peruanere i USA, Spanien, Japan og Argentina.    Udgivelsesdato: September...

  14. MADS interactomics : towards understanding the molecular mechanisms of plant MADS-domain transcription factor function

    Smaczniak, C.D.

    2013-01-01

    Protein-protein and protein-DNA interactions are essential for the molecular action of transcription factors. By combinatorial binding to target gene promoters, transcription factors are able to up- or down-regulate the expression of these genes. MADS-domain proteins comprise a large family of

  15. Interdisciplinary Critical Inquiry: Teaching about the Social Construction of Madness

    Connor-Greene, Patricia A.

    2006-01-01

    Theories and treatments of mental illness reflect the social, philosophical, and historical context in which they developed. This article describes ways to invite students to grapple with complex questions about "madness" from an interdisciplinary perspective. Looking at the construct of madness through multiple lenses (e.g., literature,…

  16. Bovine Spongiform Encephalopathy (BSE, Mad Cow Disease

    G. K. Bruckner

    1997-07-01

    Full Text Available Mad Cow Disease or BSE (Bovine Spongiform Encephalopathy became a household name internationally and also in South Africa. International hysteria resulted following reports of a possible link between a disease diagnosed in cattle in Britain and a variant of the disease diagnosed in humans after the presumed ingestion or contact with meat from infected cattle. The European Union instituted a ban on the importation of beef from the United Kingdom during March 1996 that had a severe effect on the beef industry in the UK and also resulted in a world wide consumer resistance against beef consumption.

  17. Web life: The Evil Mad Scientist Project

    2009-04-01

    What is it? Have you ever tried to electrocute a hot dog? Wondered how to make a robot out of a toothbrush, watch battery and phone-pager motor? Seen a cantaloupe melon and thought, "Hmm, I could make this look like the Death Star from the original Star Wars films"? If you have not, but you would like to - preferably as soon as you can find a pager motor - then this is the site for you. The Evil Mad Scientist Project (EMSP) blog is packed full of ideas for unusual, silly and frequently physics-related creations that bring science out of the laboratory and into kitchens, backyards and tool sheds.

  18. 213 SIG et distribution spatiale des infrastructures hydrauliques ...

    CARMELLE

    Une frange importante de la population de cette commune continue de souffrir de cette .... présente sous quatre ensembles à savoir : les forêts claires et formations boisées, les formations ..... barrage Hachef (Maroc) par télédétection et SIG".

  19. CHI 2013 Human Work Interaction Design (HWID) SIG

    Clemmensen, Torkil; Campos, Pedro F.; Katre, Dinesh S.

    2013-01-01

    In this SIG we aim to introduce the IFIP 13.6 Human Work Interaction Design (HWID) approach to the CHI audience. The HWID working group aims at establishing relationships between extensive empirical work-domain studies and HCI design. We invite participants from industry and academia with an inte...

  20. Radioimmunoassay and evaluation for serum SIgA and CG of the hepatopathy patients

    Liu Junchi; Du Shujun; Lu Jun; Tang Xiaolin; Mao Shaorong

    1995-01-01

    Serum SIgA and CG of 247 hepatopathy (male 140, female 99) are radioimmunoassayed. The Clinical Significance of SIgA, CG for hepatisms is evaluated. The results show that the assay of SIgA, CG has an important significance for the doctor to choose the treatment plans and determine the prognosis

  1. Fag Men: Mad Men, Homosexuality and Televisual Style

    Wallace, Lee

    2012-01-01

    Among the many retro-fittings achieved by Mad Men—Matthew Weinerʼs still unfurling television series set in the advertising world of the early 1960s—is the representation of the homosexual closet as a thing of the past. This essay approaches Mad Men’s account of the homophobic past in order to think about sexuality and televisual style. A landmark programme coterminous with American television transferring from analogue to digital signal, Mad Men allegorizes another moment in television histo...

  2. 'The verses of madness': schizophrenia and poetry.

    Hankir, Ahmed Khaldoon; Holloway, David; Agius, Mark; Zaman, Rashid

    2012-12-20

    In the early 19th century, Lombroso introduced the concept of hereditary taint to describe the coexistence of 'madness' and creativity. In a recent investigation, Rust et al reported a study designed to test the traditionally assumed relationship between creativity and schizophrenia. They uncovered an association between creative originality and the positive cognitive aspects of schizotypal thinking. Poetry is not only the 'product' of psychopathology but it can also be utilised as a form of therapy: "My name is David Holloway, I am a 33 year old poet/blogger with paranoid schizophrenia. A poet called Charles Bukowski has described poetry as the 'ultimate psychiatrist', and I am a firm believer in this. The strongest part of my personality is my belief in the power of love. My recovery has relied heavily on medication, diet and exercise. However it is the power of poetry that has been my true inspiration."

  3. Recent developments in MAD version 8

    Grote, H.; Iselin, F.C.

    1990-01-01

    In view of experience with the language decoder and memory manager, large parts of MAD have been rewritten recently. The dynamic memory manager is replaced by the CERN-written ZEBRA package which has been used successfully in more than 100 other programs for HEP data analysis. A new dynamic table handler keeps large tables in memory as long as they fit; it dumps them on disk or to the Cray SSD (solid-state storage device) if necessary. Tables are accessed by simple subroutine calls in a manner transparent to the user. The input language has been extended to use a more object-oriented approach. Accelerator elements are described in terms of classes of objects, providing easy selection of single elements in a large machine. A powerful mechanism has been implemented for writting tables of selected optical functions and/or element parameters in selected positions of the machine. These tables are generated and written under control of the table handler, and can be read directly by the LEP control system. MAD contains a plot module with various options to plot data from internal tables. The tables can also be fed into a stand-alone plot program. At present the following program modulus are complete: Command Decoder; Optical Functions Calculation; Closed-Orbit Correction; Harmon (chromaticity calculation); Plotting. It is foreseen that by the end of 1989 the following other modules will be ready: Matching, including some new features; Lie Algebraic Analysis: Tracking by TRANSPORT and Lie Algebraic Methods; Electron Beam Parameters; Polarization. (orig.)

  4. Cøliaki og mad uden gluten

    Borre, Mette; Rasholt, Mia; Rasmussen, Jüri Johannes

    Denne pjece henvender sig til dig, der hos lægen eller på sygehuset har fået konstateret cøliaki, eller som har et barn med cøliaki. Pjecen kan også bruges, når familie, venner, daginstitution, skole og andre skal informeres om sygdommen. Cøliaki er en sygdom i tyndtarmen. Sygdommen behandles liv....... Begynder man på diæten, inden diagnosen er stillet, begynder tyndtarmen at hele, og man risikerer at skulle spise gluten i en længere periode igen, før diagnosen kan stilles. Det kan være svært, hvis man har fået det bedre under diæten....

  5. Child-Computer Interaction SIG: Ethics and Values

    Hourcade, Juan Pablo; Zeising, Anja; Iversen, Ole Sejer

    2017-01-01

    This SIG will provide child computer interaction researchers and practitioners an opportunity to discuss topics related to ethical challenges in the design, and use of interactive technologies for children. Topics include the role of big data, the impact of technology in children’s social...... and physical ecosystem, and the consideration of ethics in children’s participation in the design of technologies, and in the conceptualization of technologies for children....

  6. Sigüenza y el alma del paisaje

    Juan Navarro de San Pío

    2017-09-01

    Full Text Available Este artículo propone un análisis de la estética del paisaje en la obra del escritor Gabriel Miró. Para ello se investiga el significado y valor atribuido al paisaje en la trilogía novelesca  (Del vivir, Libro de Sigüenza, Años y Leguas de Sigüenza, héroe modernista de su paisaje literario, así como en el ensayo Sigüenza y el mirador azul. La visión del paisaje en Miró se reconstruye a través del diálogo con diferentes fuentes culturales y filosóficas (especialmente, Giner de los Ríos y Ortega y Gasset. El paisaje mironiano muestra así la evolución desde una inicial actitud panteísta hacia una posterior mirada fenomenológica y hermenéutica.

  7. Regressão linear geograficamente ponderada em ambiente SIG

    Luís Eduardo Ximenes Carvalho

    2009-10-01

    Full Text Available

    Este artigo aborda considerações teóricas e resultados da implementação em ambiente SIG de um modelo confirmatório de estatística espacial — regressão linear geograficamente ponderada (RGP — não disponível em ambiente livre. Os aspectos teóricos deste modelo local de regressão espacial foram amplamente discutidos em virtude da escassa bibliografia existente. O modelo RGP foi implementado na linguagem de programação GISDK do SIG-T TransCAD, utilizando compreensivamente as ferramentas de manipulação, tratamento georreferenciado dos dados e rotinas de análise espacial disponibilizadas em plataformas SIG. Ao final, espera-se ter desenvolvido, ainda que de maneira parcial, uma importante ferramenta que contribuirá para a compreensão e refinamento da modelagem de fenômenos geográficos tão amplamente analisados em estudos de Planejamento de Transportes.

  8. Bovine Spongiform Encephalopathy (BSE), or Mad Cow Disease

    ... the CDC Bovine Spongiform Encephalopathy (BSE), or Mad Cow Disease Note: Javascript is disabled or is not ... spongiform encephalopathy) is a progressive neurological disorder of cattle that results from infection by an unusual transmissible ...

  9. VLT-MAD Observations of Trumpler 14

    Rochau, B.; Brandner, W.; Stolte, A.; Henning, T.; da Rio, N.; Gennaro, M.; Hormuth, F.; Marchetti, E.; Amico, P.

    We present H and KS observations of the young massive cluster Trumpler 14 obtained with the MCAO system VLT-MAD which provides homogeneous Strehl ratios over a large field of view. We derived maximum Strehl ratios of 9.8% and 12.6% with mean Strehl ratios of 6.0% and 5.9% with in H and K_S, respectively, revealing significant improvement of the spatial PSF stability compared to SCAO systems. We detected 2-3 times more sources than comparable seeing-limited observations, and comparison of the colour-magnitude diagram with isochrones reveals a very young cluster that originated in a starburst-like event 1 ± 0.5 Myr ago. We also tentatively detect hints for an older population of 3 Myr. The mass function between 0.25 Msun and 3.2 Msun appears as a broken power law with a change of the power law slope at m_c ˜ 0.53+0.12-0.10 Msun. Shallow power law slopes are found to be Gamma_{PD,PMS1} = ˜ 0.48 ± 0.11 above m_c and Gamma_{PD,PMS2} = 0.68 ± 0.30 below m_c.

  10. California and Irony in Mad Men

    Rodney Taveira

    2012-09-01

    Full Text Available The combination of melodramatic and art cinematic techniques and influences in AMC’s television series Mad Men (2007¬– reveals how a melodramatic televisuality can image novel modes of social and intimate relations and an alternative to the archetypal American narrative of the self-made man. Set in 1960s’ America, the series uses a contemporaneous and cosmopolitan California to triangulate the formal and narrative insistence of the past on the present. This triangulation is played out by Don Draper’s relations with his family, women, and his former identities and by the representation of homosexuality throughout the series. The application of Lee Edelman’s concept of “sinthomosexuality” and Richard Rorty’s “liberal ironist” reveal a queer, visual rhetoric to the show’s narrative and formal structures, forming a queer irony that allows the show to straddle the aesthetic extremes of “quality TV” (Jane Feuer and soap opera, which, in turn, queers the exemplary American heterosexuality of Don Draper.

  11. The design of the MAD Design Program

    Niederer, J.

    1992-01-01

    The study of long term stability in particle accelerators has long been served by a group of widely circulated computer programs. The progress in these programs has mirrored the growth and versatility in accelerator size, complexity, and purpose, as well as evolving technologies in computing software and hardware. A number of large accelerator projects during the last decade were designed with the aid of physics programs either written for, or tailored for the project at hand, each invariably benefiting from contributions of previous workers. This paper outlines the recent history of of expample of an accelerator lattice model tool kit, the Methodical Accelerator Design (MAD) Program, which has tried to knit together this collective wisdom of the accelerator community, The ideas behind the software design of the program itself are traced here; the accelerator physics contents and origins are thoroughly documented elsewhere. These informal notes have a Brookhaven flavor, in part because of early BNL efforts to generalize the ways that technical problems are organized and presented to computers. Some recent BNL applications not covered in the extensive CERN documentation are also included

  12. Fallstudie SIG - Supply Chain Prototyp mit Coca Cola Beverages

    Senger, Enrico

    2003-01-01

    SIG, ein führender Lieferant von Verpackungsmaterialien für Getränke, hat mit Coca Cola CPFR, collaborative planning, forecasting and replenishment realisiert. Das Unternehmen kann elektronisch und ohne Zeitverzug (vorher mit bis zu 15 Tagen Verspätung) auf die Lagerbestände und Verkaufsvorhersagen bei Coca Cola zugreifen. Coca Cola erhält die exakten Liefermengen und zeiten. Coca Cola konnte dadurch den Lagerbestand von Verpackungsmaterial um 50% senken. Dies reduziert die Bearbeitungszeit d...

  13. Sistemas integrados de gestión (SIG)

    García Pantigozo, Manuel; Quispe Atúncar, Carlos; Ráez Guevara, Luis

    2014-01-01

    La cultura de la normalización es necesaria para competir globalmente, y para esto la ISO ha elaborado una serie de sistemas de gestión orientados a las calidad, medio ambiente, seguridad en el trabajo y recursos humanos, esto significa la necesidad de integrar sistemas mediante el Sistema integrado de Gestión - SIG The culture of standardization is necessary to compete globally, and to this ISO has developed a number of management systems geared to quality, environment, occupational safe...

  14. Mapping bipolar worlds: lived geographies of 'madness' in autobiographical accounts.

    Chouinard, Vera

    2012-03-01

    This article aims to advance our understanding of women's and men's experiences of negotiating bipolar 'madness' in society and space. It addresses gaps in the clinical literature on life with bipolar and geographic accounts of 'madness' and psycho-emotional distress by considering altered ways of being in place that bipolar 'madness' entails and how narrative sense is made of these. Conceptually, I build on Cosgrove's (2000) approach to psycho-emotional distress and geographic insights about being 'mad' in place. Methodologically and empirically, I draw on thematic narrative analysis of autobiographies of living with bipolar. Key findings include altered paradoxically (dis)embodied ways of being-in-place, 'fractured' or 'whole' senses of self and ways of relating to people/places, 'straddling' 'real' and 'delusional' worlds and bipolar ways of negotiating places are not straightforwardly 'irrational'. While narrative accounts most often invoke dominant discourses about bipolar, sometimes these are challenged through 'rescripting' and 'revaluing mad' identities and ways of being in place. In conclusion, key findings and avenues for future geographical research are discussed. Copyright © 2011 Elsevier Ltd. All rights reserved.

  15. SIG-VISA: Signal-based Vertically Integrated Seismic Monitoring

    Moore, D.; Mayeda, K. M.; Myers, S. C.; Russell, S.

    2013-12-01

    Traditional seismic monitoring systems rely on discrete detections produced by station processing software; however, while such detections may constitute a useful summary of station activity, they discard large amounts of information present in the original recorded signal. We present SIG-VISA (Signal-based Vertically Integrated Seismic Analysis), a system for seismic monitoring through Bayesian inference on seismic signals. By directly modeling the recorded signal, our approach incorporates additional information unavailable to detection-based methods, enabling higher sensitivity and more accurate localization using techniques such as waveform matching. SIG-VISA's Bayesian forward model of seismic signal envelopes includes physically-derived models of travel times and source characteristics as well as Gaussian process (kriging) statistical models of signal properties that combine interpolation of historical data with extrapolation of learned physical trends. Applying Bayesian inference, we evaluate the model on earthquakes as well as the 2009 DPRK test event, demonstrating a waveform matching effect as part of the probabilistic inference, along with results on event localization and sensitivity. In particular, we demonstrate increased sensitivity from signal-based modeling, in which the SIGVISA signal model finds statistical evidence for arrivals even at stations for which the IMS station processing failed to register any detection.

  16. SIG XX - A generation of intelligent gamma ray probes

    Rusu, Al.; Bartos, D.; Constantin, F.; Caragheopol, Gh.; Cruceru, I.; Lupu, A.; Serbina, L.

    2003-01-01

    Nowadays, the radioprotection activities are governed by the ALARA principle. To comply with, we have decided to use scintillators, due to their high efficiency. The surface mounted devices allow the design of the entire gross gamma ray measuring system into a volume of about 0.5 liters. The microcontrollers having an EPROM of 4k bytes offer the opportunity to run resident programmes dedicated to: data acquisition, local processing, data transmission, and system supervising. Such an intelligence is embedded into SIG XX probes. By designing an array of such probes, one can easily obtain a portal monitor, an area monitor and so on, each of them under the the control of a PC. A few modifications may transform an intelligent probe into a portable instrument for radioprotection. In such a case, to make the probe shorter, replacing the photomultiplier by a photodiode, is an attractive goal. To reach it, a dedicated charge preamplifier has to be developed. The works and results on SIG XX probes and charge preamplifier are reported. (authors)

  17. SIG XX - a generation of intelligent gamma ray probes

    Rusu, Al.; Bartos, D.; Constantin, F.; Caragheopol, Gh; Cruceru, I.; Lupu, A.; Serbina, L.

    2005-01-01

    Full text: Nowadays, the radioprotection activities are governed by the ALARA principle. To comply with, we have decided to use scintillators, due to their large efficiency. The surface mounted devices allow the design of the entire gross gamma ray measuring system into a volume of about 0.5 liters. The microcontrollers having an EPROM of 4k bytes offer the opportunity to run resident programmes dedicated to data acquisition, local processing, data communication, system supervising. Such an intelligence is embedded into SIG XX probes. By designing an array of such probes, one can easily obtain a portal monitor, an area monitor and so on, each of them under the the control of a PC. A few modifications may transform an intelligent probe into a portable instrument for radioprotection. In such a case, to make the probe shorter, replacing the photomultiplier by a photodiode, is an attractive goal. To reach it, a dedicated charge preamplifier has to be developed. The works and results on SIG XX probes and charge preamplifier are reported. (author)

  18. [Madness in the German cinema (1913-1933].

    Aulas, J J

    1980-01-01

    During these twenty years, from 1913 to 1933, of the history of the German cinema, the cinematographic representation of madness varies according to the fluctuations of the social and economical background. The political and ideological chaos of the immediate post-war years was symbolized in the allegorical imagery of unreason in the expressionist cinema. The same equivalence, the same symbolization can be found in the cinema of the thirties when the crash of Wall-Street foretells a crisis like the former. On the contrary in the course of the so-called "relative stabilization" (1924-1929) the meaning of the representation of madness is totally different from the representation of the previous period. At this period of economical restoration, madness which could henceforth be cured on the psychoanalyst's couch (acc. G. W. Pabst's film: "Geheimnisse einer Seele") became the symbol of the absolute power rediscovered by Germany.

  19. 33 CFR 147.839 - Mad Dog Truss Spar Platform safety zone.

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Mad Dog Truss Spar Platform... SECURITY (CONTINUED) OUTER CONTINENTAL SHELF ACTIVITIES SAFETY ZONES § 147.839 Mad Dog Truss Spar Platform safety zone. (a) Description. Mad Dog Truss Spar Platform, Green Canyon 782 (GC 782), located at position...

  20. MADS box genes expressed in developing inflorescences of rice and sorghum

    Greco, R.; Stagi, L.; Colombo, L.; Angenent, G.C.; Sari-Gorla, M.; Pé, M.E.

    1997-01-01

    With the aim of elucidating the complex genetic system controlling flower morphogenesis in cereals, we have characterized two rice and two sorghum MADS box genes isolated from cDNA libraries made from developing inflorescences. The rice clones OsMADS24 and OsMADS45, which share high homology with

  1. [The representation of madness in William Shakespeare's characters].

    Stompe, Thomas; Ritter, Kristina; Friedmann, Alexander

    2006-08-01

    Shakespeare is one of the great creators of human characters of the 16(th) century. Like for many of his contemporaries madness was a central topic of his work. The first part of this paper discusses the sociocultural environment and the semantic field of madness in the Elizabethan age, which forms the background for Shakespeare's characters. In the second part we try to analyze the clinical pictures of the fictive characters of Othello, Hamlet, Lear and Macbeth. While we find melancholy, delusions and hallucinations, other diseases such as schizophrenia are missing entirely. Schizophrenia only appears in the literature more than two hundred years later, in the beginning of modern age.

  2. Don, Betty and Jackie Kennedy: On Mad Men and Periodisation

    Prudence Black

    2012-09-01

    Full Text Available Why is it that we watch Mad Men and think it represents a period? Flashes of patterned wallpaper, whiskey neat, babies born that are never mentioned, contact lining for kitchen drawers, Ayn Rand, polaroids, skinny ties, Hilton hotels, Walter Cronkite, and a time when Don Draper can ask ‘What do women want?’ and dry old Roger Sterling can reply ‘Who Cares?’ This essay explores the embrace of period detail in Mad Men finding it to be both loving and fetishistic, and belonging, like all period film, to the politics of the present.

  3. Snapshot of SIG: A Look at Four States' Approaches to School Turnaround

    Quillin, Jessica

    2012-01-01

    Thousands of schools across the country are chronically low performing, and they operate within districts and states that are struggling to help them improve. The School Improvement Grants (SIG) program is designed to channel federal funds to states and districts facing the task of turning around struggling schools. SIG, a part of the Elementary…

  4. 77 FR 24192 - SIG Energy, LLLP v. California Independent System Operator Corporation; Notice of Complaint

    2012-04-23

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. EL12-55-000] SIG Energy, LLLP v. California Independent System Operator Corporation; Notice of Complaint Take notice that on.... 824(e) and 825(e), SIG Energy, LLLP (Complainant) filed a formal complaint against the California...

  5. The Size of the Family – A Factor Influencing Alimentation and, Implicitly, the Growth and Development Processes in Teen-Agers

    Angela Simalcsik

    2011-10-01

    Full Text Available Viewed as a phenomenon with multi-factorial determination, the growth and development of the human organism depend equally on the hereditary patrimony and on the environmental conditions, the socio-economic ones, especially. As a critical transition period from childhood to adult age, adolescence is characterized by increased nutritional needs. The main factor responsible for the variations manifested in human physical development is alimentation – a factor correlated with the socio-economic variations. Similarly with the case of other countries affected by transitions, the difficult economic transformations occurring in the Republic of Moldova have been accompanied by the installation of poverty – a phenomenon affecting especially the families formed of more than 5 members. The teen-agers of the Republic of Moldova register a deficit in the consumption of proteins, especially of animal origin, known as playing a critical role in the period of accelerated growing of the organism. Alimentation and family size are closely correlated with the socio-economic status of the family from which the child comes, while the attention and care given to the child by the family plays an essential part in the growth and development of the teen-ager. Child’s development as a function of such an element is directly related to environmental factors, as follows: alimentation is poorer in more numerous families, as the monthly income per member of family is more reduced, which is immediately reflected in a scarce alimentary consumption; hygiene is insufficient; mother’s care for the child is also reduced, etc. The study was performed on 1,525 subjects (boys and girls with ages between 10 and 16 years, all from the city of Kishinev. The main parameters considered were: stature, weight and average puberal age. The results obtained show that family size influences both the average stature of the teenagers and their weight, over the 10-16 years interval, the

  6. Molecular cloning, identification, and chromosomal localization of two MADS box genes in peach (Prunus persica).

    Zhang, Lin; Xu, Yong; Ma, Rongcai

    2008-06-01

    MADS box proteins play an important role in floral development. To find genes involved in the floral transition of Prunus species, cDNAs for two MADS box genes, PpMADS1 and PpMADS10, were cloned using degenerate primers and 5'- and 3'-RACE based on the sequence database of P. persica and P. dulcis. The full length of PpMADS1 cDNA is 1,071 bp containing an open reading frame (ORF) of 717 bp and coding for a polypeptide of 238 amino acid residues. The full length of PpMADS10 cDNA is 937 bp containing an ORF of 633 bp and coding for a polypeptide of 210 amino acid residues. Sequence comparison revealed that PpMADS1 and PpMADS10 were highly homologous to genes AP1 and PI in Arabidopsis, respectively. Phylogenetic analysis indicated that PpMADS1 belongs to the euAP1 clade of class A, and PpMADS10 is a member of GLO/PI clade of class B. RT-PCR analysis showed that PpMADS1 was expressed in sepal, petal, carpel, and fruit, which was slightly different from the expression pattern of AP1; PpMADS10 was expressed in petal and stamen, which shared the same expression pattern as PI. Using selective mapping strategy, PpMADS1 was assigned onto the Bin1:50 on the G1 linkage group between the markers MCO44 and TSA2, and PpMADS10 onto the Bin1:73 on the same linkage group between the markers Lap-1 and FGA8. Our results provided the basis for further dissection of the two MADS box gene function.

  7. BioSig: the free and open source software library for biomedical signal processing.

    Vidaurre, Carmen; Sander, Tilmann H; Schlögl, Alois

    2011-01-01

    BioSig is an open source software library for biomedical signal processing. The aim of the BioSig project is to foster research in biomedical signal processing by providing free and open source software tools for many different application areas. Some of the areas where BioSig can be employed are neuroinformatics, brain-computer interfaces, neurophysiology, psychology, cardiovascular systems, and sleep research. Moreover, the analysis of biosignals such as the electroencephalogram (EEG), electrocorticogram (ECoG), electrocardiogram (ECG), electrooculogram (EOG), electromyogram (EMG), or respiration signals is a very relevant element of the BioSig project. Specifically, BioSig provides solutions for data acquisition, artifact processing, quality control, feature extraction, classification, modeling, and data visualization, to name a few. In this paper, we highlight several methods to help students and researchers to work more efficiently with biomedical signals.

  8. Antibiotic resistance of canine Staphylococcus intermedius group (SIG)--practical implications.

    Chrobak, D; Kizerwetter-Swida, M; Rzewuska, M; Binek, M

    2011-01-01

    A total of 221 SIG strains were isolated from clinical samples of canine origin submitted to the Diagnostic Laboratory of the Division of Bacteriology and Molecular Biology at the Warsaw University of Life Sciences in Warsaw during the period 2006-2010. The aim of the study was to investigate the frequency of prevalence of methicillin-resistant SIG strains and to determine the MIC values of cephalotin, amoxicillin/clavulanic acid, ciprofloxacin, clindamycin, gentamicin, chloramphenicol, mupirocin for a collection of randomly selected 79 strains belonging to Staphylococcus intermedius group (SIG), including 23 mecA-positive and 56 mecA-negative strains. All isolates were identified as belonging to SIG based on their phenotypic properties and PCR amplification of S. intermedius-specific fragment of the 16S rRNA gene. The mecA gene was detected in 26 (12%) of 221 SIG strains. All tested mecA-negative SIG strains were susceptible to amoxicillin/clavulanic acid and cephalotin. One of the 56 mecA-negative SIG strains was resistant to ciprofloxacin, six (11%) to gentamicin. It was found that sixteen (29%) of 56 mecA-negative SIG strains were resistant to clindamycin. Most of the mecA-positive SIG strains were resistant to ciprofloxacin (96%), clindamycin (96%), and gentamicin (96%). Only one MRSIG strain was resistant to chloramphenicol. All examined mecA-positive SIG strains were found to be susceptible to mupirocin. Our results imply that staphylococcal multidrug resistance has become more prevalent, which could lead to difficulties in effective treatment. With some resistant strains the only therapeutic possibility are antimicrobial agents important in human medicine. New regulations for veterinary medicine concerning appropriate therapy of infections caused by multidrug-resistat staphylococci are needed.

  9. The regulation of MADS-box gene expression during ripening of banana and their regulatory interation with ethylene

    MADS-box genes (MaMADS1-6), potential components of the developmental control of ripening have been cloned from Grand Nain banana cultivar. Similarity of these genes to tomato LeRIN is very low and neither MaMADS2 nor MaMADS1 complement the tomato rin mutation. Nevertheless, the expression patterns...

  10. What to Build for Middle-Agers to Come? Attractive and Necessary Functions of Exercise-Promotion Mobile Phone Apps: A Cross-Sectional Study.

    Liao, Gen-Yih; Chien, Yu-Tai; Chen, Yu-Jen; Hsiung, Hsiao-Fang; Chen, Hsiao-Jung; Hsieh, Meng-Hua; Wu, Wen-Jie

    2017-05-25

    Physical activity is important for middle-agers to maintain health both in middle age and in old age. Although thousands of exercise-promotion mobile phone apps are available for download, current literature offers little understanding regarding which design features can enhance middle-aged adults' quality perception toward exercise-promotion apps and which factor may influence such perception. The aims of this study were to understand (1) which design features of exercise-promotion apps can enhance quality perception of middle-agers, (2) whether their needs are matched by current functions offered in app stores, and (3) whether physical activity (PA) and mobile phone self-efficacy (MPSE) influence quality perception. A total of 105 middle-agers participated and filled out three scales: the International Physical Activity Questionnaire (IPAQ), the MPSE scale, and the need for design features questionnaire. The design features were developed based on the Coventry, Aberdeen, and London-Refined (CALO-RE) taxonomy. Following the Kano quality model, the need for design features questionnaire asked participants to classify design features into five categories: attractive, one-dimensional, must-be, indifferent, and reverse. The quality categorization was conducted based on a voting approach and the categorization results were compared with the findings of a prevalence study to realize whether needs match current availability. In total, 52 multinomial logistic regression models were analyzed to evaluate the effects of PA level and MPSE on quality perception of design features. The Kano analysis on the total sample revealed that visual demonstration of exercise instructions is the only attractive design feature, whereas the other 51 design features were perceived with indifference. Although examining quality perception by PA level, 21 features are recommended to low level, 6 features to medium level, but none to high-level PA. In contrast, high-level MPSE is recommended

  11. TrMADS3, a new MADS-box gene, from a perennial species Taihangia rupestris (Rosaceae) is upregulated by cold and experiences seasonal fluctuation in expression level.

    Du, Xiaoqiu; Xiao, Qiying; Zhao, Ran; Wu, Feng; Xu, Qijiang; Chong, Kang; Meng, Zheng

    2008-06-01

    In many temperate perennial plants, floral transition is initiated in the first growth season but the development of flower is arrested during the winter to ensure production of mature flowers in the next spring. The molecular mechanisms of the process remain poorly understood with few well-characterized regulatory genes. Here, a MADS-box gene, named as TrMADS3, was isolated from the overwintering inflorescences of Taihangia rupestris, a temperate perennial in the rose family. Phylogenetic analysis reveals that TrMADS3 is more closely related to the homologs of the FLOWERING LOCUS C lineage than to any of the other MIKC-type MADS-box lineages known from Arabidopsis. The TrMADS3 transcripts are extensively distributed in inflorescences, roots, and leaves during the winter. In controlled conditions, the TrMADS3 expression level is upregulated by a chilling exposure for 1 to 2 weeks and remains high for a longer period of time in warm conditions after cold treatment. In situ hybridization reveals that TrMADS3 is predominantly expressed in the vegetative and reproductive meristems. Ectopic expression of TrMADS3 in Arabidopsis promotes seed germination on the media containing relatively high NaCl or mannitol concentrations. These data indicate that TrMADS3 in a perennial species might have its role in both vegetative and reproductive meristems in response to cold.

  12. Cloning and analysis of two Ceratopteris thalictroides MADS-box genes

    XU Daolan

    2014-06-01

    Full Text Available MADS-box transcription factors,as a large gene family,play an important role in plant growth and development,especially act as key regulators in controlling the identities of floral organs in flowering plants.They are also significant in the evolutionary revelation.In order to understand MADS-box genes,we need more information of MADS-box genes in non flowering plant.MADS-box genes of Ceratopteris thalictroides were selected to clone and analysis by using RACE method.Two MADS-box genes,designated CtMADS1 and CtMADS2 in C. thalictroides,were cloned.Analysis indicates that CtMADS1 is belonged to MIKC*-clade,while CtMADS2 is belonged to MIKCc-clade.Phylogeny suggests that these two MADS-box genes of C. thalictroides have a close relationship with flowering plants,the data indicates that at least two different MADS-box genes are homologous to floral homeotic genes existed in the last common ancestor of contemporary vascular plants.

  13. Cassandra in the Classroom: Teaching and Moral Madness

    Santoro, Doris A.

    2017-01-01

    Moral madness is a symptom of the moral violence experienced by teachers who are expected to exercise responsibility for their students and their work, but whose moral voice is misrecognized as self-interest and whose moral agency is suppressed. I conduct a feminist ethical analysis of the figure of Cassandra to examine the ways in which teachers…

  14. Reviewing the Link between Creativity and Madness: A Postmodern Perspective

    Koh, Caroline

    2006-01-01

    Researchers on creativity and psychology have long been fascinated with the high incidence of psychotic behavior amongst geniuses and individuals of exceptional creativity. The aims of this paper are first, to review the existing findings for a better insight into the socio-contextual factors underpinning the mad genius conundrum, and secondly, to…

  15. [The madness of Herakles in Euripides and Sophocles].

    Charlier, Philippe

    2003-01-01

    In the ancient Greek world madness was conceived as a punishment sent by the gods to men found guilty of various sins. Heralkes, who kills his wife Megara and their sons, is the best example of Greek literature offers of the tragic consequences of mental disease. The article conducts a medical observation of Sophocles' and Euripides' descriptions of Herakles' insanity.

  16. A National Research Project Revitalizes and Strengthens a SIG's Membership, Leadership, and the Quality of Research in the Field

    Educational Researcher, 2011

    2011-01-01

    A recent moratorium has temporarily halted the creation of new Special Interest Groups (SIGs) in the American Educational Research Association (AERA). The AERA SIG Executive Committee, the official governance body that oversees approximately 160 SIGs, requested this moratorium, which was subsequently approved by AERA Council. The purpose of the…

  17. Comprehensive Definition of the SigH Regulon of Mycobacterium tuberculosis Reveals Transcriptional Control of Diverse Stress Responses.

    Jared D Sharp

    Full Text Available Expression of SigH, one of 12 Mycobacterium tuberculosis alternative sigma factors, is induced by heat, oxidative and nitric oxide stresses. SigH activation has been shown to increase expression of several genes, including genes involved in maintaining redox equilibrium and in protein degradation. However, few of these are known to be directly regulated by SigH. The goal of this project is to comprehensively define the Mycobacterium tuberculosis genes and operons that are directly controlled by SigH in order to gain insight into the role of SigH in regulating M. tuberculosis physiology. We used ChIP-Seq to identify in vivo SigH binding sites throughout the M. tuberculosis genome, followed by quantification of SigH-dependent expression of genes linked to these sites and identification of SigH-regulated promoters. We identified 69 SigH binding sites, which are located both in intergenic regions and within annotated coding sequences in the annotated M. tuberculosis genome. 41 binding sites were linked to genes that showed greater expression following heat stress in a SigH-dependent manner. We identified several genes not previously known to be regulated by SigH, including genes involved in DNA repair, cysteine biosynthesis, translation, and genes of unknown function. Experimental and computational analysis of SigH-regulated promoter sequences within these binding sites identified strong consensus -35 and -10 promoter sequences, but with tolerance for non-consensus bases at specific positions. This comprehensive identification and validation of SigH-regulated genes demonstrates an extended SigH regulon that controls an unexpectedly broad range of stress response functions.

  18. Comprehensive Definition of the SigH Regulon of Mycobacterium tuberculosis Reveals Transcriptional Control of Diverse Stress Responses.

    Sharp, Jared D; Singh, Atul K; Park, Sang Tae; Lyubetskaya, Anna; Peterson, Matthew W; Gomes, Antonio L C; Potluri, Lakshmi-Prasad; Raman, Sahadevan; Galagan, James E; Husson, Robert N

    2016-01-01

    Expression of SigH, one of 12 Mycobacterium tuberculosis alternative sigma factors, is induced by heat, oxidative and nitric oxide stresses. SigH activation has been shown to increase expression of several genes, including genes involved in maintaining redox equilibrium and in protein degradation. However, few of these are known to be directly regulated by SigH. The goal of this project is to comprehensively define the Mycobacterium tuberculosis genes and operons that are directly controlled by SigH in order to gain insight into the role of SigH in regulating M. tuberculosis physiology. We used ChIP-Seq to identify in vivo SigH binding sites throughout the M. tuberculosis genome, followed by quantification of SigH-dependent expression of genes linked to these sites and identification of SigH-regulated promoters. We identified 69 SigH binding sites, which are located both in intergenic regions and within annotated coding sequences in the annotated M. tuberculosis genome. 41 binding sites were linked to genes that showed greater expression following heat stress in a SigH-dependent manner. We identified several genes not previously known to be regulated by SigH, including genes involved in DNA repair, cysteine biosynthesis, translation, and genes of unknown function. Experimental and computational analysis of SigH-regulated promoter sequences within these binding sites identified strong consensus -35 and -10 promoter sequences, but with tolerance for non-consensus bases at specific positions. This comprehensive identification and validation of SigH-regulated genes demonstrates an extended SigH regulon that controls an unexpectedly broad range of stress response functions.

  19. Region 7 Significant Ecological Resource Areas (ECO_RES.SIG_REGIONS)

    U.S. Environmental Protection Agency — SIG_REGIONS is a boundary layer that displays Region 7's Significant Ecological Resource Areas. This layer represents large areas within which different ecosystem...

  20. Music, madness and the body: symptom and cure.

    MacKinnon, Dolly

    2006-03-01

    Building on Sander L. Gilman's exemplary work on images of madness and the body, this article examines images of music, madness and the body by discussing the persistent cultural beliefs stemming from Classical Antiquity that underpin music as medicinal. These images reflect the body engaged in therapeutic musical activities, as well as musical sounds forming part of the evidence of the mental diagnostic state of a patient in case records. The historiography of music as medicinal has been overlooked in the history of psychiatry. This article provides a brief background to the cultural beliefs that underlie examples of music as both symptom and cure in 19th- and 20th-century asylum records in Australia, Britain, Europe and North America.

  1. The world of Mad Men: power, surface and passion.

    Gerson, Mary-Joan

    2011-12-01

    Mad Men is disturbing to post-millennium viewers, particularly those of a "certain" age, on three counts. First, it invokes a particular historical context of gender oppression; second it captures the prevailing post-War injunction that emotional distress is unseemly and distasteful; and third, it captures the zeitgeist's celebration of surface over substance in relationship. However, just as disturbing as these historically situated interpersonal premises is the niggling question that each relationship pairing and each episode leaves with the viewer. To wit: How much of the disconnection and the unrequited longings are reflective of a particular historical era, and to what degree do they reflect timeless aspects of character and relationship? Thus Mad Men provides an exquisitely rendered sociocultural tableau in which the viewer struggles, however articulated or not, with one of the essential knots of psychoanalytic as well as couples treatment: the complicated interpenetration of culture and character, of time and timelessness.

  2. Extensions of MAD Version 8 to Include Beam Acceleration

    Raubenheimer, Tor O

    2000-01-01

    In this paper, the authors describe modifications to MAD version 8.23 to include linear accelerator cavities and beam acceleration. An additional energy variable has been added which is modified as the beam passes through LCAV elements (linear accelerator cavities) and can be used as a constraint in matching commands. The calculation of the beta functions and phase advance is consistent with that in other codes that treat acceleration such as TRANSPORT or DIMAD. These modifications allow this version of MAD to be used for the design and modeling of linacs and the authors present examples from the Next Linear Collider design as well as a muon acceleration complex. The code is available from CERN or SLAC

  3. Betaler det sig? Fleksibilitet og løn i den danske flexicurity-model

    Ibsen, Flemming

    2007-01-01

    Indkomstsikkerhed er at bevæge sig fra et job til at andet og få en højere løn, men er det tilfældet i dan danske flexicurity-model, betaler det sig at være fleksibel? Belønnes numerisk ekstern fleksibiblitet højrere end intern funktionel fleksibilbitet? Artiklen viser, at det bedst kan betale si...

  4. Slow ventricular response atrial fibrillation related to mad honey poisoning

    Osken, A.; Yaylacı, S.; Aydın, E.; Kocayigit, İ; Cakar, M.A.; Tamer, A.; Gündüz, H.

    2012-01-01

    Mad honey poisoning which is induced by Grayanotoxin (Andromedotoxin), is also known to have adverse effects in the cardiovascular system leading to different clinical entities. This toxin is produced by a member of the Rhododendron genus of plants of two R. Luteum and R. Panticum. In this article, we presented a case of slow ventricular response atrial fibrillation complaints with nausea, vomiting, dizziness and chest pain about an hour after eating honey produced in the Black Sea Region. PMID:22923947

  5. Slow ventricular response atrial fibrillation related to mad honey poisoning

    Osken, A.; Yaylacı, S.; Aydın, E.; Kocayigit, İ; Cakar, M.A.; Tamer, A.; Gündüz, H.

    2012-01-01

    Mad honey poisoning which is induced by Grayanotoxin (Andromedotoxin), is also known to have adverse effects in the cardiovascular system leading to different clinical entities. This toxin is produced by a member of the Rhododendron genus of plants of two R. Luteum and R. Panticum. In this article, we presented a case of slow ventricular response atrial fibrillation complaints with nausea, vomiting, dizziness and chest pain about an hour after eating honey produced in the Black Sea Region.

  6. Fag Men: Mad Men, Homosexuality and Televisual Style

    Lee Wallace

    2012-09-01

    Full Text Available Among the many retro-fittings achieved by Mad Men—Matthew Weinerʼs still unfurling television series set in the advertising world of the early 1960s—is the representation of the homosexual closet as a thing of the past. This essay approaches Mad Men’s account of the homophobic past in order to think about sexuality and televisual style. A landmark programme coterminous with American television transferring from analogue to digital signal, Mad Men allegorizes another moment in television history when the medium was defined not by convergence and time-shifting but by liveness, scheduling flow, mass-market demographics and synchronous viewing. Though it confines its gay content to minor characters and narrative arcs that phase in and out in relation to open-ended long-form needs, the programme’s representation of homophobia as a thing of the past provides a useful lens on the complex temporal co-ordinates of contemporary television.

  7. Prognosis End-Time: Madness and Prophecy in Melancholia and Take Shelter

    Briohny Doyle

    2013-05-01

    Full Text Available This paper discusses two films released in 2011, Lars Von Trier's Melancholia and Jeff Nichols Take Shelter in the context of the history of dominant conceptions of madness as laid out in Foucault's History of Madness. I argue that these films allegorise protagonists' experiences of depression and schizophrenia in order to critique the present moment in North America as well as to restore a poetic link between madness, prophecy and apocalypse in the popular imagination.

  8. The regulation of MADS-box gene expression during ripening of banana and their regulatory interaction with ethylene.

    Elitzur, Tomer; Vrebalov, Julia; Giovannoni, James J; Goldschmidt, Eliezer E; Friedman, Haya

    2010-03-01

    Six MaMADS-box genes have been cloned from the banana fruit cultivar Grand Nain. The similarity of these genes to tomato LeRIN is low and neither MaMADS2 nor MaMADS1 complement the tomato rin mutation. Nevertheless, the expression patterns, specifically in fruit and the induction during ripening and in response to ethylene and 1-MCP, suggest that some of these genes may participate in ripening. MaMADS1, 2, and 3, are highly expressed in fruit only, while the others are expressed in fruit as well as in other organs. Moreover, the suites of MaMADS-box genes and their temporal expression differ in peel and pulp during ripening. In the pulp, the increase in MaMADS2, 3, 4, and 5 expression preceded an increase in ethylene production, but coincides with the CO(2) peak. However, MaMADS1 expression in pulp coincided with ethylene production, but a massive increase in its expression occurred late during ripening, together with a second wave in the expression of MaMADS2, 3, and 4. In the peel, on the other hand, an increase in expression of MaMADS1, 3, and to a lesser degree also of MaMADS4 and 2 coincided with an increase in ethylene production. Except MaMADS3, which was induced by ethylene in pulp and peel, only MaMADS4, and 5 in pulp and MaMADS1 in peel were induced by ethylene. 1-MCP applied at the onset of the increase in ethylene production, increased the levels of MaMADS4 and MaMADS1 in pulp, while it decreased MaMADS1, 3, 4, and 5 in peel, suggesting that MaMADS4 and MaMADS1 are negatively controlled by ethylene at the onset of ethylene production only in pulp. Only MaMADS2 is neither induced by ethylene nor by 1-MCP, and it is expressed mainly in pulp. Our results suggest that two independent ripening programs are employed in pulp and peel which involve the activation of mainly MaMADS2, 4, and 5 and later on also MaMADS1 in pulp, and mainly MaMADS1, and 3 in peel. Hence, our results are consistent with MaMADS2, a SEP3 homologue, acting in the pulp upstream of the

  9. The vocabulary of madness from Homer to Hippocrates. Part 1: the verbal group of mualphaiotanuomicronmualphaiota.

    Perdicoyianni-Paléologou, Hélène

    2009-09-01

    In Part 1 of this two-part paper, I examine the evolution of the concept of madness expressed by the various forms--verbal and nominal, simple and compound--of the verbal group of mualphaiotanuomicronmualphaiota in the archaic and classical periods. I point out how the divine madness is contrasted to pathological madness considered as a psychic and mental disease and foreseeable by doctors as well as curable by medications. This new procedure highlights rational knowledge of the Greeks about the cause and the medical care of madness.

  10. SigB is a dominant regulator of virulence in Staphylococcus aureus small-colony variants.

    Mitchell, Gabriel; Fugère, Alexandre; Pépin Gaudreau, Karine; Brouillette, Eric; Frost, Eric H; Cantin, André M; Malouin, François

    2013-01-01

    Staphylococcus aureus small-colony variants (SCVs) are persistent pathogenic bacteria characterized by slow growth and, for many of these strains, an increased ability to form biofilms and to persist within host cells. The virulence-associated gene expression profile of SCVs clearly differs from that of prototypical strains and is often influenced by SigB rather than by the agr system. One objective of this work was to confirm the role of SigB in the control of the expression of virulence factors involved in biofilm formation and intracellular persistence of SCVs. This study shows that extracellular proteins are involved in the formation of biofilm by three SCV strains, which, additionally, have a low biofilm-dispersing activity. It was determined that SigB activity modulates biofilm formation by strain SCV CF07-S and is dominant over that of the agr system without being solely responsible for the repression of proteolytic activity. On the other hand, the expression of fnbA and the control of nuclease activity contributed to the SigB-dependent formation of biofilm of this SCV strain. SigB was also required for the replication of CF07-S within epithelial cells and may be involved in the colonization of lungs by SCVs in a mouse infection model. This study methodically investigated SigB activity and associated mechanisms in the various aspects of SCV pathogenesis. Results confirm that SigB activity importantly influences the production of virulence factors, biofilm formation and intracellular persistence for some clinical SCV strains.

  11. Structural insights into the regulation of Bacillus subtilis SigW activity by anti-sigma RsiW.

    Shankar Raj Devkota

    Full Text Available Bacillus subtilis SigW is localized to the cell membrane and is inactivated by the tight interaction with anti-sigma RsiW under normal growth conditions. Whereas SigW is discharged from RsiW binding and thus initiates the transcription of its regulon under diverse stress conditions such as antibiotics and alkaline shock. The release and activation of SigW in response to extracytoplasmic signals is induced by the regulated intramembrane proteolysis of RsiW. As a ZAS (Zinc-containing anti-sigma family protein, RsiW has a CHCC zinc binding motif, which implies that its anti-sigma activity may be regulated by the state of zinc coordination in addition to the proteolytic cleavage of RsiW. To understand the regulation mode of SigW activity by RsiW, we determined the crystal structures of SigW in complex with the cytoplasmic domain of RsiW, and compared the conformation of the CHCC motif in the reduced/zinc binding and the oxidized states. The structures revealed that RsiW inhibits the promoter binding of SigW by interacting with the surface groove of SigW. The interaction between SigW and RsiW is not disrupted by the oxidation of the CHCC motif in RsiW, suggesting that SigW activity might not be regulated by the zinc coordination states of the CHCC motif.

  12. Evaluation of the NCPDP Structured and Codified Sig Format for e-prescriptions.

    Liu, Hangsheng; Burkhart, Q; Bell, Douglas S

    2011-01-01

    To evaluate the ability of the structure and code sets specified in the National Council for Prescription Drug Programs Structured and Codified Sig Format to represent ambulatory electronic prescriptions. We parsed the Sig strings from a sample of 20,161 de-identified ambulatory e-prescriptions into variables representing the fields of the Structured and Codified Sig Format. A stratified random sample of these representations was then reviewed by a group of experts. For codified Sig fields, we attempted to map the actual words used by prescribers to the equivalent terms in the designated terminology. Proportion of prescriptions that the Format could fully represent; proportion of terms used that could be mapped to the designated terminology. The fields defined in the Format could fully represent 95% of Sigs (95% CI 93% to 97%), but ambiguities were identified, particularly in representing multiple-step instructions. The terms used by prescribers could be codified for only 60% of dose delivery methods, 84% of dose forms, 82% of vehicles, 95% of routes, 70% of sites, 33% of administration timings, and 93% of indications. The findings are based on a retrospective sample of ambulatory prescriptions derived mostly from primary care physicians. The fields defined in the Format could represent most of the patient instructions in a large prescription sample, but prior to its mandatory adoption, further work is needed to ensure that potential ambiguities are addressed and that a complete set of terms is available for the codified fields.

  13. Number 13 / Part I. Music. 3. Mad Scenes: A Warning against Overwhelming Passions

    Marisi Rossella

    2017-03-01

    Full Text Available This study focuses on mad scenes in poetry and musical theatre, stressing that, according to Aristotle’s theory on catharsis and the Affektenlehre, they had a pedagogical role on the audience. Some mad scenes by J.S. Bach, Handel and Mozart are briefly analyzed, highlighting their most relevant textual and musical characteristics.

  14. Designing and recasting LHC analyses with MadAnalysis 5

    Conte, Eric; Fuks, Benjamin; Wymant, Chris

    2014-01-01

    We present an extension of the expert mode of the MadAnalysis 5 program dedicated to the design or reinterpretation of high-energy physics collider analyses. We detail the predefined classes, functions and methods available to the user and emphasize the most recent developments. The latter include the possible definition of multiple sub-analyses and a novel user-friendly treatment for the selection criteria. We illustrate this approach by two concrete examples: a CMS search for supersymmetric partners of the top quark and a phenomenological analysis targeting hadronically decaying monotop systems.

  15. Chlorella intake attenuates reduced salivary SIgA secretion in kendo training camp participants

    Otsuki Takeshi

    2012-12-01

    Full Text Available Abstract Background The green alga Chlorella contains high levels of proteins, vitamins, and minerals. We previously reported that a chlorella-derived multicomponent supplement increased the secretion rate of salivary secretory immunoglobulin A (SIgA in humans. Here, we investigated whether intake of this chlorella-derived supplement attenuated the reduced salivary SIgA secretion rate during a kendo training camp. Methods Ten female kendo athletes participated in inter-university 6-day spring and 4-day summer camps. They were randomized into two groups; one took placebo tablets during the spring camp and chlorella tablets during the summer camp, while the other took chlorella tablets during the spring camp and placebo tablets during the summer camp. Subjects took these tablets starting 4 weeks before the camp until post-camp saliva sampling. Salivary SIgA concentrations were measured by ELISA. Results All subjects participated in nearly all training programs, and body-mass changes and subjective physical well-being scores during the camps were comparable between the groups. However, salivary SIgA secretion rate changes were different between these groups. Salivary SIgA secretion rates decreased during the camp in the placebo group (before vs. second, middle, and final day of camp, and after the camp: 146 ± 89 vs. 87 ± 56, 70 ± 45, 94 ± 58, and 116 ± 71 μg/min, whereas no such decreases were observed in the chlorella group (121 ± 53 vs. 113 ± 68, 98 ± 69,115 ± 80, and 128 ± 59 μg/min. Conclusion Our results suggest that a use of a chlorella-derived dietary supplement attenuates reduced salivary SIgA secretion during a training camp for a competitive sport.

  16. Sigma Factor SigB Is Crucial to Mediate Staphylococcus aureus Adaptation during Chronic Infections.

    Lorena Tuchscherr

    2015-04-01

    Full Text Available Staphylococcus aureus is a major human pathogen that causes a range of infections from acute invasive to chronic and difficult-to-treat. Infection strategies associated with persisting S. aureus infections are bacterial host cell invasion and the bacterial ability to dynamically change phenotypes from the aggressive wild-type to small colony variants (SCVs, which are adapted for intracellular long-term persistence. The underlying mechanisms of the bacterial switching and adaptation mechanisms appear to be very dynamic, but are largely unknown. Here, we analyzed the role and the crosstalk of the global S. aureus regulators agr, sarA and SigB by generating single, double and triple mutants, and testing them with proteome analysis and in different in vitro and in vivo infection models. We were able to demonstrate that SigB is the crucial factor for adaptation in chronic infections. During acute infection, the bacteria require the simultaneous action of the agr and sarA loci to defend against invading immune cells by causing inflammation and cytotoxicity and to escape from phagosomes in their host cells that enable them to settle an infection at high bacterial density. To persist intracellularly the bacteria subsequently need to silence agr and sarA. Indeed agr and sarA deletion mutants expressed a much lower number of virulence factors and could persist at high numbers intracellularly. SigB plays a crucial function to promote bacterial intracellular persistence. In fact, ΔsigB-mutants did not generate SCVs and were completely cleared by the host cells within a few days. In this study we identified SigB as an essential factor that enables the bacteria to switch from the highly aggressive phenotype that settles an acute infection to a silent SCV-phenotype that allows for long-term intracellular persistence. Consequently, the SigB-operon represents a possible target to develop preventive and therapeutic strategies against chronic and therapy

  17. Sigma Factor SigB Is Crucial to Mediate Staphylococcus aureus Adaptation during Chronic Infections.

    Tuchscherr, Lorena; Bischoff, Markus; Lattar, Santiago M; Noto Llana, Mariangeles; Pförtner, Henrike; Niemann, Silke; Geraci, Jennifer; Van de Vyver, Hélène; Fraunholz, Martin J; Cheung, Ambrose L; Herrmann, Mathias; Völker, Uwe; Sordelli, Daniel O; Peters, Georg; Löffler, Bettina

    2015-04-01

    Staphylococcus aureus is a major human pathogen that causes a range of infections from acute invasive to chronic and difficult-to-treat. Infection strategies associated with persisting S. aureus infections are bacterial host cell invasion and the bacterial ability to dynamically change phenotypes from the aggressive wild-type to small colony variants (SCVs), which are adapted for intracellular long-term persistence. The underlying mechanisms of the bacterial switching and adaptation mechanisms appear to be very dynamic, but are largely unknown. Here, we analyzed the role and the crosstalk of the global S. aureus regulators agr, sarA and SigB by generating single, double and triple mutants, and testing them with proteome analysis and in different in vitro and in vivo infection models. We were able to demonstrate that SigB is the crucial factor for adaptation in chronic infections. During acute infection, the bacteria require the simultaneous action of the agr and sarA loci to defend against invading immune cells by causing inflammation and cytotoxicity and to escape from phagosomes in their host cells that enable them to settle an infection at high bacterial density. To persist intracellularly the bacteria subsequently need to silence agr and sarA. Indeed agr and sarA deletion mutants expressed a much lower number of virulence factors and could persist at high numbers intracellularly. SigB plays a crucial function to promote bacterial intracellular persistence. In fact, ΔsigB-mutants did not generate SCVs and were completely cleared by the host cells within a few days. In this study we identified SigB as an essential factor that enables the bacteria to switch from the highly aggressive phenotype that settles an acute infection to a silent SCV-phenotype that allows for long-term intracellular persistence. Consequently, the SigB-operon represents a possible target to develop preventive and therapeutic strategies against chronic and therapy-refractory infections.

  18. Salivary levels of SIgA and perceived stress among dental students

    João Paulo Menck Sangiorgio

    2017-12-01

    Full Text Available Background: Academic stress may impair mucosal immunity and expose dental students to an increased risk of infections. Objective: to assess stress scores in dental students and their relationship with variation in SIgA levels. Methods: All students (n = 289 were invited to take part of the study, and 207 (71.63% effectively participated, being 152 (73.4% females. At the day of data collection, the students answered The Dental Environmental Stress Questionnaire (DES and unstimulated saliva samples were collected for determination of salivary flow rate and SIgA concentration and secretion rate. Results: Mean DES scores were higher in females (78.97 ± 16.42, but no correlations between the sum of DES scores and salivary parameters were observed (P=0.08. A moderate inverse relationship was observed between SIgA secretion rates and the subscales Academic Performance (P=0.01, Interpersonal relationships (P=0.02 and Difficulties and Insecurities about Professional Future (P=0.05. A weak correlation was found between SIgA concentration and the items Amount of assigned classwork (P=0.02, Lack of confidence in self to be a successful dentist (P=0.01, Lack of time for relaxation (P=0.01, Financial responsibilities (P=0.02 and Personal physical health (P=0.005. Weak correlations between SIgA secretion rates and DES items were also found for Lack of cooperation by patient in their home care (P=0.003, Patients being late or not showing up for their appointments (P=0.02, Lack of self confidence to be a successful dentist (P=0.008, Personal physical health (P=0.019, and others. Conclusion: Different sources of stress were observed among first to fifth year students and some of these stressors may negatively impact on salivary SIgA secretion.

  19. Status of MAD (version 8.5) and future plans

    Iselin, F.C.

    1993-01-01

    The MAD computer code project was started in 1981 at the European Organization for Nuclear Research (CERN) to provide a flexible framework for particle optical computations. From the beginning various features were considered important. Open-ended and modular design of the program would allow easy addition of new features. Format-free input language would facilitate data preparation. Later the requirement was added that the language should be understood by a variety of other programs. When moving data to other programs this would avoid extensive translations of the data with the corresponding danger of making errors. The internal data structure would describe the machine in a flexible manner and access to this structure should be simple. The program should be easy to maintain. Canonical variables should be used throughout the computations. All these features should not hamper the computational efficiency. This article describes the features of the MAD code. This is followed by two sections giving a general description of the ''standard input language'' and extensions to this language. Finally the data structures introduced and future plans are summarized. (Author)

  20. Uso di gvSIG e SEXTANTE per la perimetrazione degli ambiti periurbani

    Gabriele Nolè

    2010-03-01

    Full Text Available Use of gvSIG and SEXTANTE for the perimetration of periurban areasThe periurban fringe is the portion of land with characteristics of urbanization that cannot be considered neither urban nor rural. These areas are often characterized by a building expectancy, whose detection requires careful consideration of several territorial and environmental variables. It was implemented using a model of spatial analysis based on kernel Density Estimation (KDE for the detection of periurban areas. The model is tested in the province of Potenza using gvSIG and SEXTANTE on Ubuntu Linux.

  1. Uso di gvSIG e SEXTANTE per la perimetrazione degli ambiti periurbani

    Gabriele Nolè

    2010-03-01

    Full Text Available Use of gvSIG and SEXTANTE for the perimetration of periurban areas The periurban fringe is the portion of land with characteristics of urbanization that cannot be considered neither urban nor rural. These areas are often characterized by a building expectancy, whose detection requires careful consideration of several territorial and environmental variables. It was implemented using a model of spatial analysis based on kernel Density Estimation (KDE for the detection of periurban areas. The model is tested in the province of Potenza using gvSIG and SEXTANTE on Ubuntu Linux.

  2. « Éléments pour un état des lieux sur les SIG et SIG-P »

    L'objectif général de ce programme panafricain est de contribuer à rendre disponibles des systèmes d'information de bonne qualité, fiables et accessibles grâce à l'usage de SIG-P en vue d'améliorer la gestion des ressources naturelles (eau, terres, forêts, etc.) et de promouvoir la sécurité alimentaire. Le programme ...

  3. Monitoring the ethanol stress response of a sigM deletion strain of B. cereus ATCC 14579.

    Voort, van der M.

    2008-01-01

    Here, the role of σM and its regulon in stress response and survival of B. cereus ATCC 14579 was assessed by comparative transciptome and phenotypic analysis of this strain and its sigM deletion strain. Exposure of B. cereus ATCC 14579 to a wide range of stresses revealed expression of sigM,

  4. Report on BAAL "Language in Africa" SIG Meetings Reading in African Languages: Developing Literacies and Reading Methodologies

    Wildsmith-Cromarty, Rosemary

    2015-01-01

    This report describes ongoing research on reading in African languages. It draws mainly on contributions from two British Association for Applied Linguistics (BAAL) "Language in Africa" (LiA) Special Interest Group (SIG) meetings: the LiA SIG strand at BAAL 2013 and the seminar on "Reading Methodologies in African Languages"…

  5. Involvement of CNOT3 in mitotic progression through inhibition of MAD1 expression

    Takahashi, Akinori [Division of Oncology, Department of Cancer Biology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108-8639 (Japan); Kikuguchi, Chisato [Cell Signal Unit, Okinawa Institute of Science and Technology, Kunigami, Okinawa 904-0412 (Japan); Morita, Masahiro; Shimodaira, Tetsuhiro; Tokai-Nishizumi, Noriko; Yokoyama, Kazumasa; Ohsugi, Miho; Suzuki, Toru [Division of Oncology, Department of Cancer Biology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108-8639 (Japan); Yamamoto, Tadashi, E-mail: tyamamot@ims.u-tokyo.ac.jp [Division of Oncology, Department of Cancer Biology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108-8639 (Japan); Cell Signal Unit, Okinawa Institute of Science and Technology, Kunigami, Okinawa 904-0412 (Japan)

    2012-03-09

    Highlights: Black-Right-Pointing-Pointer CNOT3 depletion increases the mitotic index. Black-Right-Pointing-Pointer CNOT3 inhibits the expression of MAD1. Black-Right-Pointing-Pointer CNOT3 destabilizes the MAD1 mRNA. Black-Right-Pointing-Pointer MAD1 knockdown attenuates the CNOT3 depletion-induced mitotic arrest. -- Abstract: The stability of mRNA influences the dynamics of gene expression. The CCR4-NOT complex, the major deadenylase in mammalian cells, shortens the mRNA poly(A) tail and contributes to the destabilization of mRNAs. The CCR4-NOT complex plays pivotal roles in various physiological functions, including cell proliferation, apoptosis, and metabolism. Here, we show that CNOT3, a subunit of the CCR4-NOT complex, is involved in the regulation of the spindle assembly checkpoint, suggesting that the CCR4-NOT complex also plays a part in the regulation of mitosis. CNOT3 depletion increases the population of mitotic-arrested cells and specifically increases the expression of MAD1 mRNA and its protein product that plays a part in the spindle assembly checkpoint. We showed that CNOT3 depletion stabilizes the MAD1 mRNA, and that MAD1 knockdown attenuates the CNOT3 depletion-induced increase of the mitotic index. Basing on these observations, we propose that CNOT3 is involved in the regulation of the spindle assembly checkpoint through its ability to regulate the stability of MAD1 mRNA.

  6. Involvement of CNOT3 in mitotic progression through inhibition of MAD1 expression

    Takahashi, Akinori; Kikuguchi, Chisato; Morita, Masahiro; Shimodaira, Tetsuhiro; Tokai-Nishizumi, Noriko; Yokoyama, Kazumasa; Ohsugi, Miho; Suzuki, Toru; Yamamoto, Tadashi

    2012-01-01

    Highlights: ► CNOT3 depletion increases the mitotic index. ► CNOT3 inhibits the expression of MAD1. ► CNOT3 destabilizes the MAD1 mRNA. ► MAD1 knockdown attenuates the CNOT3 depletion-induced mitotic arrest. -- Abstract: The stability of mRNA influences the dynamics of gene expression. The CCR4–NOT complex, the major deadenylase in mammalian cells, shortens the mRNA poly(A) tail and contributes to the destabilization of mRNAs. The CCR4–NOT complex plays pivotal roles in various physiological functions, including cell proliferation, apoptosis, and metabolism. Here, we show that CNOT3, a subunit of the CCR4–NOT complex, is involved in the regulation of the spindle assembly checkpoint, suggesting that the CCR4–NOT complex also plays a part in the regulation of mitosis. CNOT3 depletion increases the population of mitotic-arrested cells and specifically increases the expression of MAD1 mRNA and its protein product that plays a part in the spindle assembly checkpoint. We showed that CNOT3 depletion stabilizes the MAD1 mRNA, and that MAD1 knockdown attenuates the CNOT3 depletion-induced increase of the mitotic index. Basing on these observations, we propose that CNOT3 is involved in the regulation of the spindle assembly checkpoint through its ability to regulate the stability of MAD1 mRNA.

  7. Physiological roles of sigma factor SigD in Corynebacterium glutamicum

    Taniguchi, H.; Busche, T.; Patschkowski, T.; Niehaus, K.; Pátek, Miroslav; Kalinowski, J.; Wendisch, V.F.

    2017-01-01

    Roč. 17, č. 158 (2017), s. 158 ISSN 1471-2180 R&D Projects: GA ČR(CZ) GA17-06991S Institutional support: RVO:61388971 Keywords : Corynebacterium glutamicum * Sigma factor * SigD Subject RIV: EE - Microbiology, Virology OBOR OECD: Microbiology Impact factor: 2.644, year: 2016

  8. Federal School Improvement Grants (SIGs): How Capacity and Local Conditions Matter

    Yatsko, Sarah; Lake, Robin; Bowen, Melissa; Cooley Nelson, Elizabeth

    2015-01-01

    In 2009, the federal government committed over $3 billion nationwide to help states and districts turn around their worst-performing schools. The U.S. Department of Education intended for the School Improvement Grants (SIGs) to spur dramatic change.This report looks at the results of a field study of the first-year implementation of those grants…

  9. TRPV1 channels and the progesterone receptor Sig-1R interact to regulate pain.

    Ortíz-Rentería, Miguel; Juárez-Contreras, Rebeca; González-Ramírez, Ricardo; Islas, León D; Sierra-Ramírez, Félix; Llorente, Itzel; Simon, Sidney A; Hiriart, Marcia; Rosenbaum, Tamara; Morales-Lázaro, Sara L

    2018-02-13

    The Transient Receptor Potential Vanilloid 1 (TRPV1) ion channel is expressed in nociceptors where, when activated by chemical or thermal stimuli, it functions as an important transducer of painful and itch-related stimuli. Although the interaction of TRPV1 with proteins that regulate its function has been previously explored, their modulation by chaperones has not been elucidated, as is the case for other mammalian TRP channels. Here we show that TRPV1 physically interacts with the Sigma 1 Receptor (Sig-1R), a chaperone that binds progesterone, an antagonist of Sig-1R and an important neurosteroid associated to the modulation of pain. Antagonism of Sig-1R by progesterone results in the down-regulation of TRPV1 expression in the plasma membrane of sensory neurons and, consequently, a decrease in capsaicin-induced nociceptive responses. This is observed both in males treated with a synthetic antagonist of Sig-1R and in pregnant females where progesterone levels are elevated. This constitutes a previously undescribed mechanism by which TRPV1-dependent nociception and pain can be regulated.

  10. 73 Utilisation du SIG pour une réorganisation urbaine du centre-ville ...

    TOHOZIN

    restructuration des routes, du chemin de fer, des bâtis et des cours d'eaux. ... aussi la réalisation d'une mappe foncière qui est un instrument capital en matière ..... confirmer que le SIG, en tant qu'outil de décision et de planification a ...

  11. Incorporating Early Learning Strategies in the School Improvement Grants (SIG) Program

    Connors-Tadros, Lori; Dunn, Lenay; Martella, Jana; McCauley, Carlas

    2015-01-01

    The Center on Enhancing Early Learning Outcomes (CEELO) and the Center on School Turnaround (CST) collaborated to develop case studies of three selected schools receiving SIG funds that have, with the support of their districts, promoted the use of early childhood programming (PK-3) as a key strategy in their schools' turnaround models. The goal…

  12. Unge er en flok egoistiske individualister, der har nok i sig selv

    Kofod, Anne

    2005-01-01

     Nutidens unge har som oftest en tvetydig identitet: På én og samme tid søger de sammen i sociale fællesskaber og gør alt for at skille sig ud som individualister.   Udgivelsesdato: 27. september...

  13. Domogis: prototipo de un interfaz del sistema de control de un edificio integrado en un SIG

    Álvarez, M.

    2010-06-01

    Full Text Available This paper deals with of a the use of Geographical Information Systems (GIS for domotic control. The foccus is put on the communication interface between the building control system (BCS integrated in a GIS. For get this aim, the GIS of the Montegancedo Campus where is located the Facultad de infomatica of UPM and the creation of an interface is needed. The implemented interface in Microsoft C# language allows the control, monotorizing and management of the sensors data installated in the Campus.

    Este trabajo trata de la utilización de los Sistemas de Información Geográfica (SIG en uno de las nuevos requerimientos de la arquitectura, el control domótico. El objetivo es el desarrollo de un interfaz de comunicación del Sistema de Control de un Edificio (SCE integrado en un SIG. La consecución de este objetivo implica previamente el desarrollo del SIG del Campus de Montegancedo sede de la Facultad de Informática de la UPM y la creación de un interfaz integrado en el SIG, desarrollado en lenguaje de programacion C# de Microsoft. Este interfaz dirige al usuario en la realización de ciertas tareas de control domótico de las instalaciones urbanas y edificios del Campus universitario, como evaluar, monotorizar y gestionar datos procedentes de sensores estratégicamente situados en dicho Campus.

  14. ASIST SIG/CR Classification Workshop 2000: Classification for User Support and Learning.

    Soergel, Dagobert

    2001-01-01

    Reports on papers presented at the 62nd Annual Meeting of ASIST (American Society for Information Science and Technology) for the Special Interest Group in Classification Research (SIG/CR). Topics include types of knowledge; developing user-oriented classifications, including domain analysis; classification in the user interface; and automatic…

  15. BioSig3D: High Content Screening of Three-Dimensional Cell Culture Models.

    Cemal Cagatay Bilgin

    Full Text Available BioSig3D is a computational platform for high-content screening of three-dimensional (3D cell culture models that are imaged in full 3D volume. It provides an end-to-end solution for designing high content screening assays, based on colony organization that is derived from segmentation of nuclei in each colony. BioSig3D also enables visualization of raw and processed 3D volumetric data for quality control, and integrates advanced bioinformatics analysis. The system consists of multiple computational and annotation modules that are coupled together with a strong use of controlled vocabularies to reduce ambiguities between different users. It is a web-based system that allows users to: design an experiment by defining experimental variables, upload a large set of volumetric images into the system, analyze and visualize the dataset, and either display computed indices as a heatmap, or phenotypic subtypes for heterogeneity analysis, or download computed indices for statistical analysis or integrative biology. BioSig3D has been used to profile baseline colony formations with two experiments: (i morphogenesis of a panel of human mammary epithelial cell lines (HMEC, and (ii heterogeneity in colony formation using an immortalized non-transformed cell line. These experiments reveal intrinsic growth properties of well-characterized cell lines that are routinely used for biological studies. BioSig3D is being released with seed datasets and video-based documentation.

  16. Proceedings of the 10th ASIS SIG/CR classification research workshop

    This volume is a working copy of the papers presented at the 9th ASIS SIG/CR workshop on classification research, held in Washington, DC, at the ASIS Annual Meeting on Sunday 31 October 1999. The contributions printed here are working papers, and thus, not necessarily in their final form...

  17. The horror of madness in Estrella distante and Nocturno de Chile

    Bernardo Rocco

    2017-03-01

    Full Text Available The article examines the thematic dimension of madness present in the novels Estrella distante and Nocturno de Chile by Roberto Bolaño in order to determine the various ways by which the narrator subjectivity develops a critical consciousness of madness from the traumatic experience of the Chilean dictatorship. Also, this dimension evidences the articulation of a language of madness as literary aesthetics of artistic experience of limits: the horror. Accordingly, a preliminary analysis of contemporary history as a meeting place between violent media versus artistic media that account for the chiaroscuro of Latin American modernity is offered.

  18. GENDERED DOLLARS: Pin Money, Mad Money, and Changing Notions of a Woman’s Proper Place

    Janice Traflet

    2008-01-01

    Full Text Available This essay examines the evolution in the meaning and usage of two types of special currencies: pin money and mad money. At the start of the twentieth century, both currencies were considered a woman’s money. By the end of the century, however, both pin money and mad money had lost a large measure of their original gendered connotations. By situating the evolving meanings of these currencies alongside concepts of domesticity, virtuous womanhood, and a woman’s proper place, this essay strives to illuminate the rise and fall of pin money and mad money as uniquely “women’s dollars." 

  19. SigG Does Not Control Gene Expression in Response to DNA Damage in Mycobacterium tuberculosis H37Rv ▿ §

    Smollett, Katherine L.; Dawson, Lisa F.; Davis, Elaine O.

    2010-01-01

    Expression of the Mycobacterium tuberculosis sigG sigma factor was induced by a variety of DNA-damaging agents, but inactivation of sigG did not affect induction of gene expression or bacterial survival under these conditions. Therefore, SigG does not control the DNA repair response of M. tuberculosis H37Rv.

  20. Mad Men’s Deceptive (Critique Of Creativity

    Julie Robert

    2012-09-01

    Full Text Available This article analyses Mad Men’s relationship to creativity. Considering popular, industry-specific and scholarly understandings, it uses close readings of the show and its narratological techniques to demonstrate how these potentially contradictory concepts and practices of creativity overlap in the show’s fourth season. The points at which these understandings collide become sources of tension between characters and are marked by narrative gaps that conceal deceptive creativity. The conflicts centre on three primary debates: a the role of alcohol in the creative process, b industry-specific norms of creativity, and c the popular perception that creativity is about expression. Consequently, this article approaches questions about creativity using the show’s own partially elided debates and undermines widely-held romantic beliefs about the creative ‘type’ and the what exactly it means to sell creativity in a corporate setting.

  1. Sanctified madness: the God-intoxicated saints of Bengal.

    Morinis, A

    1985-01-01

    The saintly madman is a familiar character in South Asia. To outer appearances he is no different from a lunatic, but the mad saint comes to be revered because his idiocy is popularly believed to arise from a different cause than ordinary madness. The common psychopath neglects social conventions because his consciousness is dimmed by incapacity; the saintly madman also breaches convention, but does so because his heightened consciousness has liberated him from the bonds of convention that entrap ordinary people. In the terms of Hinduism, he has tasted the divine nectar of God-realization and has returned to the human realm intoxicated by the experience. In this paper two popular God intoxicated saints of Bengal are discussed. The question is posed whether 'God intoxication' can be considered a culture-bound syndrome of Bengal. The concept of 'culture bound syndrome' is found to be too narrow to encompass the most significant issues to arise from reflection on the characteristics of the God intoxicated. These larger issues have to do with the relationship between cultural practices and models and mental states (whether deviant, as implied by the term 'syndrome' although deviance does not always carry the negative connotation implicit in 'syndrome', or normal). It is suggested that all cultures culture a limited range of mental states and thus the questions posed by the notion of culture bound syndromes are subsumed by larger questions about the relationship of all mind-states to the socio-cultural environment which conditions them. The conclusion is that God intoxication is indeed a uniquely Bengali mental condition, with variants throughout South Asia and kinship to other mystical states, but that the concept of 'syndrome' is not useful.

  2. The response of Bacillus licheniformis to heat and ethanol stress and the role of the SigB regulon.

    Voigt, Birgit; Schroeter, Rebecca; Jürgen, Britta; Albrecht, Dirk; Evers, Stefan; Bongaerts, Johannes; Maurer, Karl-Heinz; Schweder, Thomas; Hecker, Michael

    2013-07-01

    The heat and ethanol stress response of Bacillus licheniformis DSM13 was analyzed at the transcriptional and/or translational level. During heat shock, regulons known to be heat-induced in Bacillus subtilis 168 are upregulated in B. licheniformis, such as the HrcA, SigB, CtsR, and CssRS regulon. Upregulation of the SigY regulon and of genes controlled by other extracytoplasmic function (ECF) sigma factors indicates a cell-wall stress triggered by the heat shock. Furthermore, tryptophan synthesis enzymes were upregulated in heat stressed cells as well as regulons involved in usage of alternative carbon and nitrogen sources. Ethanol stress led to an induction of the SigB, HrcA, and CtsR regulons. As indicated by the upregulation of a SigM-dependent protein, ethanol also triggered a cell wall stress. To characterize the SigB regulon of B. licheniformis, we analyzed the heat stress response of a sigB mutant. It is shown that the B. licheniformis SigB regulon comprises additional genes, some of which do not exist in B. subtilis, such as BLi03885, encoding a hypothetical protein, the Na/solute symporter gene BLi02212, the arginase homolog-encoding gene BLi00198 and mcrA, encoding a protein with endonuclease activity. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  3. Dectin-1 is essential for reverse transcytosis of glycosylated SIgA-antigen complexes by intestinal M cells.

    Nicolas Rochereau

    2013-09-01

    Full Text Available Intestinal microfold (M cells possess a high transcytosis capacity and are able to transport a broad range of materials including particulate antigens, soluble macromolecules, and pathogens from the intestinal lumen to inductive sites of the mucosal immune system. M cells are also the primary pathway for delivery of secretory IgA (SIgA to the gut-associated lymphoid tissue. However, although the consequences of SIgA uptake by M cells are now well known and described, the mechanisms whereby SIgA is selectively bound and taken up remain poorly understood. Here we first demonstrate that both the Cα1 region and glycosylation, more particularly sialic acid residues, are involved in M cell-mediated reverse transcytosis. Second, we found that SIgA is taken up by M cells via the Dectin-1 receptor, with the possible involvement of Siglec-5 acting as a co-receptor. Third, we establish that transcytosed SIgA is taken up by mucosal CX3CR1⁺ dendritic cells (DCs via the DC-SIGN receptor. Fourth, we show that mucosal and systemic antibody responses against the HIV p24-SIgA complexes administered orally is strictly dependent on the expression of Dectin-1. Having deciphered the mechanisms leading to specific targeting of SIgA-based Ag complexes paves the way to the use of such a vehicle for mucosal vaccination against various infectious diseases.

  4. Emotional and deliberative reactions to a public crisis: Mad Cow disease in France.

    Sinaceur, Marwan; Heath, Chip; Cole, Steve

    2005-03-01

    Although most theories of choice are cognitive, recent research has emphasized the role of emotions. We used a novel context--the Mad Cow crisis in France--to investigate how emotions alter choice even when consequences are held constant. A field study showed that individuals reduced beef consumption in months after many newspaper articles featured the emotional label "Mad Cow," but beef consumption was unaffected after articles featured scientific labels for the same disease. The reverse pattern held for the disease-related actions of a government bureaucracy. A lab study showed that the Mad Cow label induces people to make choices based solely on emotional reactions, whereas scientific labels induce people to consider their own probability judgments. Although the Mad Cow label produces less rational behavior than scientific labels, it is two to four times more common in the environment.

  5. Changes in ethylene signaling and MADS box gene expression are associated with banana finger drop.

    Hubert, O; Piral, G; Galas, C; Baurens, F-C; Mbéguié-A-Mbéguié, D

    2014-06-01

    Banana finger drop was examined in ripening banana harvested at immature (iMG), early (eMG) and late mature green (lMG) stages, with contrasting ripening rates and ethylene sensitivities. Concomitantly, 11 ethylene signal transduction components (ESTC) and 6 MADS box gene expressions were comparatively studied in median (control zone, CZ) and pedicel rupture (drop zone DZ) areas in peel tissue. iMG fruit did not ripen or develop finger drop while eMG and lMG fruits displayed a similar finger drop pattern. Several ESTC and MADS box gene mRNAs were differentially induced in DZ and CZ and sequentially in eMG and lMG fruits. MaESR2, 3 and MaEIL1, MaMADS2 and MaMADS5 had a higher mRNA level in eMG and acted earlier, whereas MaERS1, MaCTR1, MaEIL3/AB266319, MaEIL4/AB266320 and MaEIL5/AB266321, MaMADS4 and to a lesser extent MaMADS2 and 5 acted later in lMG. In this fruit, MaERS1 and 3, MaCTR1, MaEIL3, 4 and MaEIL5/AB266321, and MaMADS4 were enhanced by finger drop, suggesting their specific involvement in this process. MaEIL1, MaMADS1 and 3, induced at comparable levels in DZ and CZ, are probably related to the overall fruit ripening process. These findings led us to consider that developmental cues are the predominant finger drop regulation factor. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

  6. The Bacillus subtilis yaaH Gene Is Transcribed by SigE RNA Polymerase during Sporulation, and Its Product Is Involved in Germination of Spores

    Kodama, Takeko; Takamatsu, Hiromu; Asai, Kei; Kobayashi, Kazuo; Ogasawara, Naotake; Watabe, Kazuhito

    1999-01-01

    The expression of 21 novel genes located in the region from dnaA to abrB of the Bacillus subtilis chromosome was analyzed. One of the genes, yaaH, had a predicted promoter sequence conserved among SigE-dependent genes. Northern blot analysis revealed that yaaH mRNA was first detected from 2 h after the cessation of logarithmic growth (T2) of sporulation in wild-type cells and in spoIIIG (SigG−) and spoIVCB (SigK−) mutants but not in spoIIAC (SigF−) and spoIIGAB (SigE−) mutants. The transcript...

  7. A novel compartment, the 'subqpical stem' of the aerial hyphae, is the location of a sigN-dependent, developmentally distinct transcription in Streptomyces coelicolor.

    Dalton, KA; Thibessard, A; Hunter, JI; Kelemen, GH

    2007-01-01

    Streptomyces coelicolor has nine SigB-like RNA polymerase sigma factors, several of them implicated in morphological differentiation and/or responses to different stresses. One of the nine, SigN, is the focus of this article. A constructed sigN null mutant was delayed in development and exhibited a bald phenotype when grown on minimal medium containing glucose as carbon source. One of two distinct sigN promoters, sigNP1, was active only during growth on solid medium, when its activation coinc...

  8. libLocation: acceso a dispositivos de localización para gvSIG Desktop y Mobile

    Jordán Aldasorro, Juan G.; Planells Jiménez, Manuel

    2009-01-01

    Inicialmente integrada en el piloto de gvSIG Mobile, la librería libLocation tiene como objetivo dotar a los proyectos gvSIG Desktop y gvSIG Mobile un acceso transparente a fuentes de localización. La librería se fundamenta en las especificaciones JSR-179 -API de localización para J2ME- y JSR-293 -API de localización para J2ME v2.0-, proporcionando una interfaz uniforme a diferentes fuentes de localización, mediante funciones de alto nivel. Asimismo, se extiende la funcionalida...

  9. Selenide isotope generators for the Galileo Mission: SIG hermetic bimetal weld transition joint

    Barnett, W.J.

    1979-08-01

    The successful development of the commercial 6061-T651/Silver/304L explosive clad plate material as a bimetal weld transition joint material, as described herein, satisfies all SIG Galileo design requirements for hermetic weld attachment of stainless steel subassemblies to aluminum alloy generator housing or end cover structures. The application of this type weld transition joint to the hermetic attachment of stainless steel shell connectors is well-developed and tested. Based on on-going life tests of stainless steel receptacle/bimetal ring attachment assemblies and metallurgical characterization studies of this transition joint material, it appears evident that this transition joint material has more than adequate capability to meet the 250 to 300 0 F and 50,000 hr. design life of the SIG/Galileo mission. Its extended life temperture capability may well approach 350 to 400 0 F

  10. Rapport fra projektet ”At skrive sig til læsning”

    Labuz, Nadia; Bundsgaard, Jeppe; Kjertmann, Kjeld

    Projektet At skrive sig til læsning er et Videnkupon-projekt finansieret af Forsknings- og Innovationsstyrelsen og firmaet Jamus. Projektet havde til formål at bidrage til viden om og kvalificere udviklingen af en app til iPad, som har til formål at støtte børn i deres skrive- og læseudvikling....... Projektet bestod konkret i diskussioner og konsulentbistand under udviklingen af app'en, og i empiriske undersøgelser af den første brug af app'en i to herboende amerikanske børnefamilier. Projektet resulterede i positiv evaluering af app'en og den praksis, der kan udvikle sig omkring brugen af den, og det...... resulterede i en række konkrete forslag til videreudvikling, samt i 10 principper for god brug af app'en....

  11. SIG EN LA NUBE: WEBSIG PARA LA ENSEÑANZA DE LA GEOGRAFÍA

    Andrew J. Milson

    Full Text Available RESUMEN:Para la mayoría de los profesores dedicados a la enseñanza de la Geografía no hay duda de que el SIG es una herramienta importante en el proceso de enseñanza-aprendizaje, pero su uso se ha ido retrasando por problemas tales como el coste del software y la gestión de grandes archivos de datos espaciales. El movimiento hacia la computación en la nube, conocida como la nube de Internet, es una tendencia prometedora para los SIG en la educación. La "nube" se refiere a una red virtual que ofrece a los usuarios acceso a archivos, servicios y aplicaciones. En este artículo se pone de manifiesto que la nube de Internet y el WebSIG tienen un gran potencial para enriquecer la educación geográfica. Se presentan tres experiencias sustentadas en el uso de estas nuevas herramientas en las aulas en los EE.UU. con las conclusiones de carácter didáctico derivadas de cada caso. PALABRAS CLAVE WEBSIG; SIG; enseñanza de la geografía; la nube de Internet; ArcGIS Online; ArcGIS Explorer Desktop (AGX. ABSTRACT There is no doubt among most geography educators that GIS is an important tool for teaching and learning, but its use has been slowed by issues such as the cost of the software and the management of large spatial data files. The move to cloud computingis one trend that is promising for GIS in education. The "cloud" refers to a virtual network that provides many users with access to files, services, and applications. In this article I argue that cloud computing and WebGIS have the potential to transform geography education. I will describe three case studies that make use of these emerging tools in classrooms in the US, and discuss the lessons that we can learn from these cases. KEY WORDS WEBGIS; GIS; cloud computing; ArcGIS Online; ArcGIS Explorer Desktop (AGX. RÉSUMÉ Il n'ya aucun doute parmi les éducateurs les plus géographie que le SIG est un outil important pour l'enseignement et l'apprentissage, mais son utilisation a

  12. DrugSig: A resource for computational drug repositioning utilizing gene expression signatures.

    Hongyu Wu

    Full Text Available Computational drug repositioning has been proved as an effective approach to develop new drug uses. However, currently existing strategies strongly rely on drug response gene signatures which scattered in separated or individual experimental data, and resulted in low efficient outputs. So, a fully drug response gene signatures database will be very helpful to these methods. We collected drug response microarray data and annotated related drug and targets information from public databases and scientific literature. By selecting top 500 up-regulated and down-regulated genes as drug signatures, we manually established the DrugSig database. Currently DrugSig contains more than 1300 drugs, 7000 microarray and 800 targets. Moreover, we developed the signature based and target based functions to aid drug repositioning. The constructed database can serve as a resource to quicken computational drug repositioning. Database URL: http://biotechlab.fudan.edu.cn/database/drugsig/.

  13. Ferramenta SIG para Modelos de Propagação de Ondas. Desenvolvimentos Preliminares

    Zózimo, A. C.; Charneca, N.; Gonçalves, A.; Fortes, C. J. E. M.

    2005-01-01

    SIMAR é um Sistema Integrado de Modelação da Agitação maRítima, para estudos de propagação e deformação da agitação marítima em zonas costeiras e portuárias. Este Sistema é baseado num Sistema de Informação Geográfica (SIG) e inclui um conjunto de módulos correspondentes aos modelos de propagação e deformação de ondas. A comunicação entre o SIG e os módulos é efectuada por uma interface gráfica construída para o efeito. Nesta comunicação, apresenta-se uma versão preliminar do SIMAR e das s...

  14. A balance of Mad and Myc expression dictates larval cell apoptosis and adult stem cell development during Xenopus intestinal metamorphosis.

    Okada, Morihiro; Miller, Thomas C; Wen, Luan; Shi, Yun-Bo

    2017-05-11

    The Myc/Mad/Max network has long been shown to be an important factor in regulating cell proliferation, death and differentiation in diverse cell types. In general, Myc-Max heterodimers activate target gene expression to promote cell proliferation, although excess of c-Myc can also induce apoptosis. In contrast, Mad competes against Myc to form Mad-Max heterodimers that bind to the same target genes to repress their expression and promote differentiation. The role of the Myc/Mad/Max network during vertebrate development, especially, the so-called postembryonic development, a period around birth in mammals, is unclear. Using thyroid hormone (T3)-dependent Xenopus metamorphosis as a model, we show here that Mad1 is induced by T3 in the intestine during metamorphosis when larval epithelial cell death and adult epithelial stem cell development take place. More importantly, we demonstrate that Mad1 is expressed in the larval cells undergoing apoptosis, whereas c-Myc is expressed in the proliferating adult stem cells during intestinal metamorphosis, suggesting that Mad1 may have a role in cell death during development. By using transcription activator-like effector nuclease-mediated gene-editing technology, we have generated Mad1 knockout Xenopus animals. This has revealed that Mad1 is not essential for embryogenesis or metamorphosis. On the other hand, consistent with its spatiotemporal expression profile, Mad1 knockout leads to reduced larval epithelial apoptosis but surprisingly also results in increased adult stem cell proliferation. These findings not only reveal a novel role of Mad1 in regulating developmental cell death but also suggest that a balance of Mad and Myc controls cell fate determination during adult organ development.

  15. The encounter with God in myth and madness.

    Doerr, Otto; Velásquez, Oscar

    2007-07-03

    It is well known how often psychiatric patients report religious experiences. These are especially frequent in schizophrenic and epileptic patients as the subject of their delusions. The question we pose is: are there differences between this kind of religious experiences and those we find in religious texts or in the mythological tradition? An overview on famous mythological narratives, such as The Aeneid, allows us to establish that the divinities become recognizable to the human being at the moment of their departure. Thus, Aeneas does not recognise his mother, Venus, when she appears to him in the middle of the forest at the coast of Africa. A dialogue between the two takes place, and only at the end of the encounter, when she is going away and already with her back to Aeneas, she shows her son the signs of her divinity: the rose-flush emanating from her neck, her hair perfume and the majesty of her gait. Something analogous can be observed in the encounter of Moses with Yahweh on Mount Sinai. Moses asks God: "Show me your glory, I beg you". And God replies, among other things: "you shall see the back of me, but my face is not to be seen". In the same sense, the Emmaus disciples do not recognise Jesus till the moment of his disappearance ("but he had vanished from their sight"), and Saul of Tars falls off his horse just in the moment when he feels the divine presence. In short, the direct encounter with the divinity seems not to occur in the realm of myth or in religious tradition. The realm of madness is exactly the opposite. Our research on religious experiences in schizophrenic and epileptic patients leads us to conclude that God appears to them face to face, and the patient describes God the father, Jesus or the Virgin Mary in intimate detail, always in an everyday setting. So, the divinity is seen in the garden, or in the bedroom, or maybe above the wardrobe, without any of its majesty. The nearness to God also tends to be so extreme that even an

  16. Mad, terroir og tv: Smag på Danmark!

    Dorthe Refslund Christensen

    2008-09-01

    Full Text Available Mad fylder mere i medierne, men hvordan underholder fx et madprogram på tv? Med afsæt i de 13 afsnit af Claus Meyer-serien Smag på Danmark! undersøger vi, hvordan serien er konstrueret og fortalt. Især ser vi på, hvilken rolle de 13 forskellige steder, som serien foregår på, har. Vi diskuterer begreber som terroir og mytologisering. Serien etablerer dobbeltheder og relationer, og i tråd med dette forlenes sted som terroir både med al og med ingen betydning. Mad knyttes som et tegn, med Barthes in mente, til stedet, men på en måde, der leverer rum for seerens eget forestillingsarbejde. Serien demonstrerer for seerne, hvordan smagsdomme kan foregå med Meyer som seerens stedfortræder i et ferie- og fritidsunivers. Den nydelse, som serien konstant etablerer, demonstrerer og anticiperer, kan seeren deltage i med sit eget fantasiarbejde: Det er god underholdning, fordi så meget er overladt til seernes forestillingsarbejde. Food, Terroir, and Television: Taste Denmark! Food has become more dominant in the media landscape, but how does a food programme on television entertain us? We analyse how the 13 episodes of the Claus Meyer series Smag på Danmark (Taste Denmark!are constructed and narrated. We pay particular attention to the different locations of the episodes and discuss the terms terroir and mythologization. The series establishes ambiguities and relations, and in this connection the location understood as terroir means everything – and at the same time means nothing at all. With reference to Barthes, the series links food as a sign to location, but in such a way that room is left for the viewer’s own imagination. The series demonstrates to the viewers how judgements of taste can be made in a time of leisure and a universe of holidays, with Meyer acting as the viewer’s proxy. The series is constantly establishing, demonstrating and anticipating a pleasure in which the viewer can participate in his or her own imagination

  17. The encounter with God in myth and madness

    Doerr Otto

    2007-07-01

    Full Text Available Abstract Background It is well known how often psychiatric patients report religious experiences. These are especially frequent in schizophrenic and epileptic patients as the subject of their delusions. The question we pose is: are there differences between this kind of religious experiences and those we find in religious texts or in the mythological tradition? Results An overview on famous mythological narratives, such as The Aeneid, allows us to establish that the divinities become recognizable to the human being at the moment of their departure. Thus, Aeneas does not recognise his mother, Venus, when she appears to him in the middle of the forest at the coast of Africa. A dialogue between the two takes place, and only at the end of the encounter, when she is going away and already with her back to Aeneas, she shows her son the signs of her divinity: the rose-flush emanating from her neck, her hair perfume and the majesty of her gait. Something analogous can be observed in the encounter of Moses with Yahweh on Mount Sinai. Moses asks God: "Show me your glory, I beg you". And God replies, among other things: "you shall see the back of me, but my face is not to be seen". In the same sense, the Emmaus disciples do not recognise Jesus till the moment of his disappearance ("but he had vanished from their sight", and Saul of Tars falls off his horse just in the moment when he feels the divine presence. In short, the direct encounter with the divinity seems not to occur in the realm of myth or in religious tradition. The realm of madness is exactly the opposite. Our research on religious experiences in schizophrenic and epileptic patients leads us to conclude that God appears to them face to face, and the patient describes God the father, Jesus or the Virgin Mary in intimate detail, always in an everyday setting. So, the divinity is seen in the garden, or in the bedroom, or maybe above the wardrobe, without any of its majesty. The nearness to God

  18. BioSig: A bioinformatic system for studying the mechanism of intra-cell signaling

    Parvin, B.; Cong, G.; Fontenay, G.; Taylor, J.; Henshall, R.; Barcellos-Hoff, M.H.

    2000-01-01

    Mapping inter-cell signaling pathways requires an integrated view of experimental and informatic protocols. BioSig provides the foundation of cataloging inter-cell responses as a function of particular conditioning, treatment, staining, etc. for either in vivo or in vitro experiments. This paper outlines the system architecture, a functional data model for representing experimental protocols, algorithms for image analysis, and the requried statistical analysis. The architecture provides...

  19. Les SIG-P au service d'une gestion durable des ressources ...

    Les SIG-P au service d'une gestion durable des ressources naturelles et de la sécurité alimentaire en Afrique. Les pays africains ont besoin de données convenables aux fins de la formulation et de la mise en oeuvre de politiques et de stratégies de sécurité alimentaire systématiques et cohérentes. Ces pays disposent de ...

  20. At Forberede sig til den mundtlige prøve ( kap12)

    Nørgaard, Britta Kusk

    2015-01-01

    Et opslagsværk for studerende til støtte for bacheloropgaveskrivning. Klæd dine studerende på til at skrive den store afsluttende opgave. De studerende får med denne bog et gedigent opslagsværk, som giver svar på alle de spørgsmål, det typisk trænger sig på, når bacheloropgaven skal påbegyndes. B...

  1. SIG et risques naturels: le glissement de terrain de Séchilienne (Isère

    Jean-Pierre ASTÉ

    1993-12-01

    Full Text Available À Séchilienne (massif de l’Oisans s’est produit un glissement de terrain qui, selon les experts, peut devenir glissement de versant entier. Leurs scénarios ont été traduits cartographiquement en un SIG qui, malgré ses limites, constitue un premier outil de prise de conscience et d’aide à la décision.

  2. Interface 3D de aplicações SIG como espaços de comunicação

    Juliano Schimiguel

    2002-01-01

    Resumo: Um Sistema de Informação Geográfica (SIG) é um sistema voltado para manipulação, gerenciamento e visualização de dados geo-referenciados. O termo geo-referenciado denota dados que possuem representação em um sistema de coordenadas geográficas. Os SIG permitem a criação de aplicações para domínios específicos, como é o caso de planejamento urbano e ambiental. Uma aplicação envolve dados, algoritmos, funções e visualização (interface de aplicação). Existem duas categorias de SIG: SIG 2D...

  3. User's guide to program MAD: a computer program for the organization and manipulation of magnetic tape directories

    Gray, W.H.

    1979-05-01

    MAD is a computer program for the organization and manipulation of the information contained in magnetic tape directories. Program MAD creates, updates, and interrogates a set of four random access files collectively called the MAD unified data base. Although program MAD was originally intended as an information compression mechanism, it has evolved into an organization system with the added feature of an approximately 60% reduction in the space required to store the data. This program is easy to use, relatively fast, efficient in its use of disk space, and available to all users of the Fusion Energy Division DECsystem-10

  4. Casein kinase II is required for the spindle assembly checkpoint by regulating Mad2p in fission yeast

    Shimada, Midori; Yamamoto, Ayumu; Murakami-Tonami, Yuko; Nakanishi, Makoto; Yoshida, Takashi; Aiba, Hirofumi; Murakami, Hiroshi

    2009-01-01

    The spindle checkpoint is a surveillance mechanism that ensures the fidelity of chromosome segregation in mitosis. Here we show that fission yeast casein kinase II (CK2) is required for this checkpoint function. In the CK2 mutants mitosis occurs in the presence of a spindle defect, and the spindle checkpoint protein Mad2p fails to localize to unattached kinetochores. The CK2 mutants are sensitive to the microtubule depolymerising drug thiabendazole, which is counteracted by ectopic expression of mad2 + . The level of Mad2p is low in the CK2 mutants. These results suggest that CK2 has a role in the spindle checkpoint by regulating Mad2p.

  5. Restoring Madness to History in J.M. Coetzee’s In the Heart of the Country

    William Collins

    2015-01-01

    Full Text Available This article interrogates the curious dismissal of madness from the critical landscape surrounding J. M. Coetzee’s In the Heart of the Country, and makes suggestions concerning how madness works in the novel and why—given certain critical and historical pressures—it has been persistently sidelined. An analysis of the novel in light of Coetzee’s scholarship on Samuel Beckett suggests that Magda’s discourse, like those of many Beckettian narrators, follows patterns of affirmation and auto-negation, constituting a fiction of what Coetzee calls “net zero.” In particular, Magda extends this pattern to the taking on and casting off of identities, perhaps in the style of the hermit crab she puts forward as an image of herself. An intertextual examination of the semantic and rhetorical range of madness as it appears in Coetzee’s other fiction and scholarship reveals that madness, for Coetzee, consistently denotes: on the one hand, a contagious force moving throughout a social body, and on the other hand, the labor of writing under the threat of illegibility—a threat conditioned in large part by the madness of the social body. By infecting the writer who might record its workings in history and thereby inhibiting or distorting that record, madness likewise appears in historical record as “net zero.” Thus, rather than simply being mad, Magda’s relationship with madness is emblematic of the (disappearance of madness in and from history.

  6. SIG y análisis espacial de datos: perspectivas convergentes

    Michael F. Goodchild

    2005-01-01

    Full Text Available En este artículo se identifican algunos de los desarrollos más importantes experimentados por los SIG y el análisis espacial de datos desde los inicios de los 50. Aunque tanto los SIG como el análisis espacial de datos comenzaron como dos áreas de investigación y aplicación más o menos separadas, han crecido unidos estrechamente a lo largo del tiempo. En el trabajo se mantiene que estas dos disciplinas se unen en el terreno de la Ciencia de la Información Geográfica, proporcionando cada una de ellas apoyo o añadiendo valor a la otra. El artículo comienza proporcionando una visión crítica retrospectiva de los desarrollos que han tenido lugar en los últimos cincuenta años. A continuación, se reflexiona acerca de los desafíos actuales y se especula sobre el futuro. Por último se comenta el potencial de convergencia del desarrollo de los SIG y del análisis espacial de datos bajo la rubrica de la Ciencia de la Información Geográfica (o SIGciencia.

  7. SIG y análisis espacial de datos: perspectivas convergentes

    Michael F. Goodchild

    2005-06-01

    Full Text Available En este artículo se identifican algunos de los desarrollos más importantes experimentados por los SIG y el análisis espacial de datos desde los inicios de los 50. Aunque tanto los SIG como el análisis espacial de datos comenzaron como dos áreas de investigación y aplicación más o menos separadas, han crecido unidos estrechamente a lo largo del tiempo. En el trabajo se mantiene que estas dos disciplinas se unen en el terreno de la Ciencia de la Información Geográfica, proporcionando cada una de ellas apoyo o añadiendo valor a la otra. El artículo comienza proporcionando una visión crítica retrospectiva de los desarrollos que han tenido lugar en los últimos cincuenta años. A continuación, se reflexiona acerca de los desafíos actuales y se especula sobre el futuro. Por último se comenta el potencial de convergencia del desarrollo de los SIG y del análisis espacial de datos bajo la rubrica de la Ciencia de la Información Geográfica (o SIGciencia.

  8. Automated software system for checking the structure and format of ACM SIG documents

    Mirza, Arsalan Rahman; Sah, Melike

    2017-04-01

    Microsoft (MS) Office Word is one of the most commonly used software tools for creating documents. MS Word 2007 and above uses XML to represent the structure of MS Word documents. Metadata about the documents are automatically created using Office Open XML (OOXML) syntax. We develop a new framework, which is called ADFCS (Automated Document Format Checking System) that takes the advantage of the OOXML metadata, in order to extract semantic information from MS Office Word documents. In particular, we develop a new ontology for Association for Computing Machinery (ACM) Special Interested Group (SIG) documents for representing the structure and format of these documents by using OWL (Web Ontology Language). Then, the metadata is extracted automatically in RDF (Resource Description Framework) according to this ontology using the developed software. Finally, we generate extensive rules in order to infer whether the documents are formatted according to ACM SIG standards. This paper, introduces ACM SIG ontology, metadata extraction process, inference engine, ADFCS online user interface, system evaluation and user study evaluations.

  9. Induction of a stable sigma factor SigR by translation-inhibiting antibiotics confers resistance to antibiotics

    Yoo, Ji-Sun; Oh, Gyeong-Seok; Ryoo, Sungweon; Roe, Jung-Hye

    2016-01-01

    Antibiotic-producing streptomycetes are rich sources of resistance mechanisms against endogenous and exogenous antibiotics. An ECF sigma factor ?R (SigR) is known to govern the thiol-oxidative stress response in Streptomyces coelicolor. Amplification of this response is achieved by producing an unstable isoform of ?R called ?R?. In this work, we present evidence that antibiotics induce the SigR regulon via a redox-independent pathway, leading to antibiotic resistance. The translation-inhibiti...

  10. Madness in blaise cendrars' novels: moravagine and company.

    Tatu, Laurent; Bogousslavsky, Julien

    2013-01-01

    The literary work of Blaise Cendrars (1887-1961), né Frédéric Sauser, one of the major French-speaking authors of the 20th century, is imbued with references to neuropsychiatry. This theme is a constant presence in his writing as a result of his involvement of the First World War and his personal experiences, which were punctuated with neurologic and psychiatric events. Cendrars' own particular ideas on the genesis of mental disorders went against the more traditional views on psychiatry. He remained skeptical about hysteria and did not subscribe to psychoanalysis. His ideas were enriched by his experience with the war-related neuropsychiatric problems developed by soldiers. He thus proposed the notion of 'pathological fear' surrounding these disorders. There are a number of characters suffering from 'borderline' mental disorders in Cendrars' work, including two shocking, mad murderers, Moravagine and Fébronio. The character Moravagine, a neurology patient suffering from a brain tumor, enabled Cendrars to delve into the grey areas that can exist between neurologic and psychiatric diseases. Fébronio, a real psychotic, enabled Cendrars to explore ethnopsychiatry. Copyright © 2013 S. Karger AG, Basel.

  11. The Extra-Cytoplasmic Function Sigma Factor SigX Modulates Biofilm and Virulence-Related Properties in Pseudomonas aeruginosa

    Gicquel, Gwendoline; Bouffartigues, Emeline; Bains, Manjeet; Oxaran, Virginie; Rosay, Thibaut; Lesouhaitier, Olivier; Connil, Nathalie; Bazire, Alexis; Maillot, Olivier; Bénard, Magalie; Cornelis, Pierre; Hancock, Robert E. W.; Dufour, Alain; Feuilloley, Marc G. J.; Orange, Nicole; Déziel, Eric; Chevalier, Sylvie

    2013-01-01

    SigX, one of the 19 extra-cytoplasmic function sigma factors of P. aeruginosa, was only known to be involved in transcription of the gene encoding the major outer membrane protein OprF. We conducted a comparative transcriptomic study between the wildtype H103 strain and its sigX mutant PAOSX, which revealed a total of 307 differentially expressed genes that differed by more than 2 fold. Most dysregulated genes belonged to six functional classes, including the “chaperones and heat shock proteins”, “antibiotic resistance and susceptibility”, “energy metabolism”, “protein secretion/export apparatus”, and “secreted factors”, and “motility and attachment” classes. In this latter class, the large majority of the affected genes were down-regulated in the sigX mutant. In agreement with the array data, the sigX mutant was shown to demonstrate substantially reduced motility, attachment to biotic and abiotic surfaces, and biofilm formation. In addition, virulence towards the nematode Caenorhabditis elegans was reduced in the sigX mutant, suggesting that SigX is involved in virulence-related phenotypes. PMID:24260387

  12. Agave tequilana MADS genes show novel expression patterns in meristems, developing bulbils and floral organs.

    Delgado Sandoval, Silvia del Carmen; Abraham Juárez, María Jazmín; Simpson, June

    2012-03-01

    Agave tequilana is a monocarpic perennial species that flowers after 5-8 years of vegetative growth signaling the end of the plant's life cycle. When fertilization is unsuccessful, vegetative bulbils are induced on the umbels of the inflorescence near the bracteoles from newly formed meristems. Although the regulation of inflorescence and flower development has been described in detail for monocarpic annuals and polycarpic species, little is known at the molecular level for these processes in monocarpic perennials, and few studies have been carried out on bulbils. Histological samples revealed the early induction of umbel meristems soon after the initiation of the vegetative to inflorescence transition in A. tequilana. To identify candidate genes involved in the regulation of floral induction, a search for MADS-box transcription factor ESTs was conducted using an A. tequilana transcriptome database. Seven different MIKC MADS genes classified into 6 different types were identified based on previously characterized A. thaliana and O. sativa MADS genes and sequences from non-grass monocotyledons. Quantitative real-time PCR analysis of the seven candidate MADS genes in vegetative, inflorescence, bulbil and floral tissues uncovered novel patterns of expression for some of the genes in comparison with orthologous genes characterized in other species. In situ hybridization studies using two different genes showed expression in specific tissues of vegetative meristems and floral buds. Distinct MADS gene regulatory patterns in A. tequilana may be related to the specific reproductive strategies employed by this species.

  13. Derrida, Foucault and “Madness, the Absence of an Œuvre”

    Seferin James

    Full Text Available This article argues that Foucault's 1964 paper “La folie, l'absence d'œuvre” ought to be understood as a response to Derrida's 1963 paper “Cogito et histoire de la folie”. I clarify the chronology of the exchange between these two thinkers and follow commentators Bennington and Flynn in emphasising themes other than the status of madness in Descartes. I undertake a thematic investigation of Foucault's 1961 characterisation of madness as the absence of an œuvre and the role of this characterisation in Derrida's 1963 paper. Then I turn to an investigation of Foucault's substantial change in position on these key themes with his 1964 paper. I argue that Foucault seeks to minimise the initial importance he attributed to his characterisation of madness as the absence of an œuvre, altering his understanding of the relation between madness and language as well as shifting the event that silences madness from Descartes to Freud. Derrida's reconsideration of Foucault's Folie et déraison in 1991 treats Freud as the new locus of the exchange. This is an implicit recognition by Derrida of Foucault's “La folie, l'absence d'œuvre” and confirmation of its place within the exchange.

  14. Translational Control of the SigR-Directed Oxidative Stress Response in Streptomyces via IF3-Mediated Repression of a Noncanonical GTC Start Codon.

    Feeney, Morgan A; Chandra, Govind; Findlay, Kim C; Paget, Mark S B; Buttner, Mark J

    2017-06-13

    The major oxidative stress response in Streptomyces is controlled by the sigma factor SigR and its cognate antisigma factor RsrA, and SigR activity is tightly controlled through multiple mechanisms at both the transcriptional and posttranslational levels. Here we show that sigR has a highly unusual GTC start codon and that this leads to another level of SigR regulation, in which SigR translation is repressed by translation initiation factor 3 (IF3). Changing the GTC to a canonical start codon causes SigR to be overproduced relative to RsrA, resulting in unregulated and constitutive expression of the SigR regulon. Similarly, introducing IF3* mutations that impair its ability to repress SigR translation has the same effect. Thus, the noncanonical GTC sigR start codon and its repression by IF3 are critical for the correct and proper functioning of the oxidative stress regulatory system. sigR and rsrA are cotranscribed and translationally coupled, and it had therefore been assumed that SigR and RsrA are produced in stoichiometric amounts. Here we show that RsrA can be transcribed and translated independently of SigR, present evidence that RsrA is normally produced in excess of SigR, and describe the factors that determine SigR-RsrA stoichiometry. IMPORTANCE In all sigma factor-antisigma factor regulatory switches, the relative abundance of the two proteins is critical to the proper functioning of the system. Many sigma-antisigma operons are cotranscribed and translationally coupled, leading to a generic assumption that the sigma and antisigma factors are produced in a fixed 1:1 ratio. In the case of sigR - rsrA , we show instead that the antisigma factor is produced in excess over the sigma factor, providing a buffer to prevent spurious release of sigma activity. This excess arises in part because sigR has an extremely rare noncanonical GTC start codon, and as a result, SigR translation initiation is repressed by IF3. This finding highlights the potential significance

  15. Hydraulic analysis, Mad River at State Highway 41, Springfield, Ohio

    Mayo, Ronald I.

    1977-01-01

    A hydraulic analysis of the lad River in a reach at Springfield, Ohio was made to determine the effects of relocating State Highway 41 in 1S76. The main channel was cleaned by dredging in the vicinity cf the new highway bridge and at the Detroit, Toledo and Ironton Railway bridge upstream. The new highway was placed on a high fill with relief structures for flood plain drainage consisting of a 12-foot corrugated metal pipe culvert and a bridge opening to accommodate the Detroit, Toledo and Ironton Railway and a property access road. The effect of the new highway embankment on drainage from the flood plain was requested. Also requested was the effect that might be expected on the elevation of flood waters above the new highway embankment if the access road through the new highway embankment were raised.The study indicates that the improvement in the capacity of the main channel to carry water was such that, up to a discharge equivalent to a 25-year frequency flood, the water-surface elevation in the reach upstream from the Detroit, Toledo and Ironton Railway bridge would be about 0.6 foot lower than under conditions prior to the construction on State Highway 41. Diversion through the Mad River left bank levee break above the Detroit, Toledo and Ironton Railway bridge to the flood Flain would be decreased about one-half in terms of rate of discharge in cubic feet per second. The maximum difference in elevation cf the flood water between the upstream and downstream side of the new State Highway 41 embankment would be about 0.2 foot, with an additional 0.4 foot to be expected if the access road were raised 1.5 feet.

  16. Multivariate Alteration Detection (MAD) and MAF Postprocessing in Multispectral, Bitemporal Image Data: New Approaches to Change Detection Studies

    Nielsen, Allan Aasbjerg; Conradsen, Knut; Simpson, James J.

    1998-01-01

    type analyses of simple difference images. Case studies with AHVRR and Landsat MSS data using simple linear stretching and masking of the change images show the usefulness of the new MAD and MAF/MAD change detection schemes. Ground truth observations confirm the detected changes. A simple simulation...

  17. Characterization of a Smad motif similar to Drosophila mad in the mouse Msx 1 promoter.

    Alvarez Martinez, Cristina E; Binato, Renata; Gonzalez, Sayonara; Pereira, Monica; Robert, Benoit; Abdelhay, Eliana

    2002-03-01

    Mouse Msx 1 gene, orthologous of the Drosophila msh, is involved in several developmental processes. BMP family members are major proteins in the regulation of Msx 1 expression. BMP signaling activates Smad 1/5/8 proteins, which associate to Smad 4 before translocating to the nucleus. Analysis of Msx 1 promoter revealed the presence of three elements similar to the consensus established for Mad, the Smad 1 Drosophila counterpart. Notably, such an element was identified in an enhancer important for Msx 1 regulation. Gel shift analysis demonstrated that proteins from 13.5 dpc embryo associate to this enhancer. Remarkably, supershift assays showed that Smad proteins are present in the complex. Purified Smad 1 and 4 also bind to this fragment. We demonstrate that functional binding sites in this enhancer are confined to the Mad motif and flanking region. Our data suggest that this Mad motif may be functional in response to BMP signaling. ©2002 Elsevier Science (USA).

  18. madSTORM: a superresolution technique for large-scale multiplexing at single-molecule accuracy

    Yi, Jason; Manna, Asit; Barr, Valarie A.; Hong, Jennifer; Neuman, Keir C.; Samelson, Lawrence E.

    2016-01-01

    Investigation of heterogeneous cellular structures using single-molecule localization microscopy has been limited by poorly defined localization accuracy and inadequate multiplexing capacity. Using fluorescent nanodiamonds as fiducial markers, we define and achieve localization precision required for single-molecule accuracy in dSTORM images. Coupled with this advance, our new multiplexing strategy, madSTORM, allows accurate targeting of multiple molecules using sequential binding and elution of fluorescent antibodies. madSTORM is used on an activated T-cell to localize 25 epitopes, 14 of which are on components of the same multimolecular T-cell receptor complex. We obtain an average localization precision of 2.6 nm, alignment error of 2.0 nm, and molecules within structures. Probing the molecular topology of complex signaling cascades and other heterogeneous networks is feasible with madSTORM. PMID:27708141

  19. Generating QCD amplitudes in the color-flow basis with MadGraph

    Hagiwara, Kaoru; Takaesu, Yoshitaro

    2011-01-01

    We propose to make use of the off-shell recursive relations with the color-flow decomposition in the calculation of QCD amplitudes on MadGraph. We introduce colored quarks and their interactions with nine gluons in the color-flow basis plus an Abelian gluon on MadGraph, such that it generates helicity amplitudes in the color-flow basis with off-shell recursive formulae for multi-gluon sub-amplitudes. We demonstrate calculations of up to 5-jet processes such as gg→5g, u anti u→5g and uu→uuggg. Although our demonstration is limited, it paves the way to evaluate amplitudes with more quark lines and gluons with MadGraph. (orig.)

  20. The Regularized Iteratively Reweighted MAD Method for Change Detection in Multi- and Hyperspectral Data

    Nielsen, Allan Aasbjerg

    2007-01-01

    This paper describes new extensions to the previously published multivariate alteration detection (MAD) method for change detection in bi-temporal, multi- and hypervariate data such as remote sensing imagery. Much like boosting methods often applied in data mining work, the iteratively reweighted...... to observations that show little change, i.e., for which the sum of squared, standardized MAD variates is small, and small weights are assigned to observations for which the sum is large. Like the original MAD method, the iterative extension is invariant to linear (affine) transformations of the original...... an agricultural region in Kenya, and hyperspectral airborne HyMap data from a small rural area in southeastern Germany are given. The latter case demonstrates the need for regularization....

  1. Menti opache. Mad Men e la rappresentazione dell’interiorità nelle serie TV

    Gianluigi Rossini

    2014-06-01

    Full Text Available One of the most visible features of Mad Men is the opposition between the lavish visual surface and the lack of depth in the description of characters. It's very difficult to empathise with Don Draper and many other Mad Men's characters, because their psychology often seems completely inaccessible or unexpressed, contrary to what happens in most contemporary TV series. The aim of this essay is to show how and why the authorial agency conveys an 'opacity effect' that clouds the spectator's understanding of the thoughts and feelings of the characters. In doing this, I will place Mad Men inside a tradition of TV series centred on the psychology and emotions of characters. The analysis will be carried out within two theoretical frameworks: James Phelan's rethorical theory of narrative and Celestino Deleyto's model of film narrative.

  2. Characterization of the SigD regulon of C. difficile and its positive control of toxin production through the regulation of tcdR.

    Imane El Meouche

    Full Text Available Clostridium difficile intestinal disease is mediated largely by the actions of toxins A (TcdA and B (TcdB, whose production occurs after the initial steps of colonization involving different surface or flagellar proteins. In B. subtilis, the sigma factor SigD controls flagellar synthesis, motility, and vegetative autolysins. A homolog of SigD encoding gene is present in the C.difficile 630 genome. We constructed a sigD mutant in C. difficile 630 ∆erm to analyze the regulon of SigD using a global transcriptomic approach. A total of 103 genes were differentially expressed between the wild-type and the sigD mutant, including genes involved in motility, metabolism and regulation. In addition, the sigD mutant displayed decreased expression of genes involved in flagellar biosynthesis, and also of genes encoding TcdA and TcdB as well as TcdR, the positive regulator of the toxins. Genomic analysis and RACE-PCR experiments allowed us to characterize promoter sequences of direct target genes of SigD including tcdR and to identify the SigD consensus. We then established that SigD positively regulates toxin expression via direct control of tcdR transcription. Interestingly, the overexpression of FlgM, a putative anti-SigD factor, inhibited the positive regulation of motility and toxin synthesis by SigD. Thus, SigD appears to be the first positive regulator of the toxin synthesis in C. difficile.

  3. MetSigDis: a manually curated resource for the metabolic signatures of diseases.

    Cheng, Liang; Yang, Haixiu; Zhao, Hengqiang; Pei, Xiaoya; Shi, Hongbo; Sun, Jie; Zhang, Yunpeng; Wang, Zhenzhen; Zhou, Meng

    2017-08-22

    Complex diseases cannot be understood only on the basis of single gene, single mRNA transcript or single protein but the effect of their collaborations. The combination consequence in molecular level can be captured by the alterations of metabolites. With the rapidly developing of biomedical instruments and analytical platforms, a large number of metabolite signatures of complex diseases were identified and documented in the literature. Biologists' hardship in the face of this large amount of papers recorded metabolic signatures of experiments' results calls for an automated data repository. Therefore, we developed MetSigDis aiming to provide a comprehensive resource of metabolite alterations in various diseases. MetSigDis is freely available at http://www.bio-annotation.cn/MetSigDis/. By reviewing hundreds of publications, we collected 6849 curated relationships between 2420 metabolites and 129 diseases across eight species involving Homo sapiens and model organisms. All of these relationships were used in constructing a metabolite disease network (MDN). This network displayed scale-free characteristics according to the degree distribution (power-law distribution with R2 = 0.909), and the subnetwork of MDN for interesting diseases and their related metabolites can be visualized in the Web. The common alterations of metabolites reflect the metabolic similarity of diseases, which is measured using Jaccard index. We observed that metabolite-based similar diseases are inclined to share semantic associations of Disease Ontology. A human disease network was then built, where a node represents a disease, and an edge indicates similarity of pair-wise diseases. The network validated the observation that linked diseases based on metabolites should have more overlapped genes. © The Author 2017. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  4. A "His Story" of Insanity: Madness and Masculinity in Twentieth-Century American Literature

    Bumeistere, Lilita

    2013-01-01

    This thesis is an interdisciplinary study of the largely neglected relationship between madness and masculinity based on three American literary works written during different periods of the twentieth century. The study utilizes literary, social, and medical research in order to provide a holistic view of madness and masculinity as two social constructs that interact with and are contingent on each other. In Sherwood Anderson’s “Hands,” Ken Kesey’s One Flew Over the Cuckoo’s Nest, and David F...

  5. The secular and the supernatural: madness and psychiatry in the short stories of Muriel Spark.

    Beveridge, A W

    2015-01-01

    Edinburgh-born Muriel Spark is one of modern Scotland's greatest writers. Examination of her work reveals that the subjects of madness and psychiatry are recurrent themes in her writing. She herself had a mental breakdown when she was a young woman and she took an interest in the world of psychiatry and psychoanalysis. In her short stories, Spark approaches the subject of madness in a variety of ways: she relates it to the supernatural; to writing fiction; and to religion. She frequently juxtaposes secular and supernatural explanations of mental disturbance. Spark adopts a sceptical and, at times, mocking view of psychiatrists and psychiatric treatment. Both psychoanalysis and pills are seen as problematic.

  6. GENDERED DOLLARS: Pin Money, Mad Money, and Changing Notions of a Woman’s Proper Place

    Janice Traflet

    2008-01-01

    This essay examines the evolution in the meaning and usage of two types of special currencies: pin money and mad money. At the start of the twentieth century, both currencies were considered a woman’s money. By the end of the century, however, both pin money and mad money had lost a large measure of their original gendered connotations. By situating the evolving meanings of these currencies alongside concepts of domesticity, virtuous womanhood, and a woman’s proper place, this essay strives t...

  7. A rare case of Kounis syndrome provoked by mad honey poisoning

    Yakup Alsancak

    2016-06-01

    Full Text Available Kounis syndrome, resulting in acute coronary syndromes, as a result of allergic or hypersensitivity reaction is triggered by several factors. Vasospasm which is mediated by mediators released after mast cell activation, is the responsible mechanism for comprising of type 2-myocardial infarction. Mad honey containing grayanotoxin is previously shown to be associated with gastrointestinal, neurological and cardiac disorders. In this case report, we presented a Kounis syndrome that has occurred after the mad honey intake and treated successfully, previously not mentioned in the literature.

  8. SIG POSTER

    income of self-employed youth. Address the underlying causes of poor educational outcomes. Tackle the barriers that limit women's potential. Build the evidence base for effective skills training programs for young people. Lasting solutions: Young women and teen girls are among the most likely to work in marginal jobs.

  9. SIG POSTER

    self-employed. 60% of youth aged 15-24 have not gone beyond primary school. Enhancing the productivity and income of youth is a key priority. Provide evidence on what interventions work, what do not, and what is scalable. Uncover the barriers to school-to-work transitions. Foster an enabling environment that spurs.

  10. SIG POSTER

    des jeunes. Tanzaniens sont sans emploi. 7 enfants sur 10qui entament leur. 3e année du primaire ne savent pas lire le swahili, la langue nationale. Seul 1 enfant sur 5 arrive à faire des mathématiques de base. Les jeunes citadins sont presque 5 fois plus susceptibles de détenir un diplôme universitaire que les jeunes.

  11. SIG POSTER

    Pour obtenir plus de renseignements, veuillez consulter Youth employment in sub-Saharan Africa: Taking stock of the evidence and knowledge gaps, par Betcherman et Khan (2015), un document d'orientation commandé par le CRDI et la Fondation MasterCard. Les jeunes ayant une formation supérieure sont deux ...

  12. SIG POSTER

    Cibler les sources de sous-emploi. Constituer une base de données probantes pour établir des mesures qui s'attaquent au chômage croissant parmi les diplômés. Évaluer l' impact des programmes existants de formation pour les jeunes. Supprimer les obstacles qui freinent l'essor des jeunes femmes. Les jeunes.

  13. SIG POSTER

    analphabètes. Créer des programmes novateurs pour accroître la productivité et les revenus des jeunes travailleurs autonomes. Traiter les causes profondes des faibles taux de réussite scolaire. Éliminer les obstacles qui limitent le potentiel des femmes. Établir une base de données probantes permettant de créer des ...

  14. Novel nuclear-encoded proteins interacting with a plastid sigma factor, Sig1, in Arabidopsis thaliana.

    Morikawa, Kazuya; Shiina, Takashi; Murakami, Shinya; Toyoshima, Yoshinori

    2002-03-13

    Sigma factor binding proteins are involved in modifying the promoter preferences of the RNA polymerase in bacteria. We found the nuclear encoded protein (SibI) that is transported into chloroplasts and interacts specifically with the region 4 of Sig1 in Arabidopsis. SibI and its homologue, T3K9.5 are novel proteins, which are not homologous to any protein of known function. The expression of sibI was tissue specific, light dependent, and developmentally timed. We suggest the transcriptional regulation by sigma factor binding proteins to function in the plastids of higher plant.

  15. Développement d'un outil SIG d'estimation des dommages ...

    Pour atteindre cet objectif, la méthodologie de simulation présentée dans le programme "HAZUS” en combinaison avec les systèmes d'information géographique (SIG) ont été utilisés. La ville a été divisée en plusieurs parties qui reflètent la typologie du tissu urbain qui à son tour n'est pas uniforme, puisque les structures ...

  16. Selenide isotope generator for the Galileo Mission: SIG/Galileo hermetic receptable test program final report

    Roedel, S.

    1979-06-01

    The purpose of the receptacle test program was to test various types of hermetically sealed electrical receptacles and to select one model as the spaceflight hardware item for SIG/Galileo thermoelectric generators. The design goal of the program was to qualify a hermetic seal integrity of less than or equal to 1 x 10 -9 std cc He/sec -atm at 400 0 F (204 0 C) and verify a reliability of 0.95 at a 50% confidence level for a flight mission in excess of 7 years

  17. sigE facilitates the adaptation of Bordetella bronchiseptica to stress conditions and lethal infection in immunocompromised mice

    Barchinger Sarah E

    2012-08-01

    Full Text Available Abstract Background The cell envelope of a bacterial pathogen can be damaged by harsh conditions in the environment outside a host and by immune factors during infection. Cell envelope stress responses preserve the integrity of this essential compartment and are often required for virulence. Bordetella species are important respiratory pathogens that possess a large number of putative transcription factors. However, no cell envelope stress responses have been described in these species. Among the putative Bordetella transcription factors are a number of genes belonging to the extracytoplasmic function (ECF group of alternative sigma factors, some of which are known to mediate cell envelope stress responses in other bacteria. Here we investigate the role of one such gene, sigE, in stress survival and pathogenesis of Bordetella bronchiseptica. Results We demonstrate that sigE encodes a functional sigma factor that mediates a cell envelope stress response. Mutants of B. bronchiseptica strain RB50 lacking sigE are more sensitive to high temperature, ethanol, and perturbation of the envelope by SDS-EDTA and certain β-lactam antibiotics. Using a series of immunocompromised mice deficient in different components of the innate and adaptive immune responses, we show that SigE plays an important role in evading the innate immune response during lethal infections of mice lacking B cells and T cells. SigE is not required, however, for colonization of the respiratory tract of immunocompetent mice. The sigE mutant is more efficiently phagocytosed and killed by peripheral blood polymorphonuclear leukocytes (PMNs than RB50, and exhibits decreased cytotoxicity toward macrophages. These altered interactions with phagocytes could contribute to the defects observed during lethal infection. Conclusions Much of the work on transcriptional regulation during infection in B. bronchiseptica has focused on the BvgAS two-component system. This study reveals that the Sig

  18. [Existence, Absence and Power of Madness: A Critical Review of Michel Foucault's Writings on the History and Philosophy of Madness].

    Brückner, Burkhart; Iwer, Lukas; Thoma, Samuel

    2017-03-01

    This article discusses Michel Foucault's main writings on "madness and psychiatry" from his early works up to the 1970s. On the one hand, we reconstruct the overall theoretical and methodological development of his positions over the course of the different periods in his oeuvre. On the other hand, we also take a closer look at Foucault's philosophical considerations regarding the subjects of his investigations. After an initial introduction of our conceptual approach, we draw on the most recent research on Foucault to show to what extent the phenomenological description of the topic at hand and the historical-critical perspective that are reflected in his early writings of 1954 (the Introduction to Binswanger's Dream and Existence and Mental Illness and Personality) laid the ground for his later work. Moving on to Foucault's work during the 1960s, we look at the core features and methodological bases of his 1961 classic Folie et déraison (History of Madness). His propositions regarding the "absence of madness" in modernity are conceptualized as an inherently contradictory attempt to liberate the topic under study from the common assumptions at that time. We then situate his 1973/74 lectures on Psychiatric Power in the context of his shift towards analyzing the dynamics of power and highlight the renewed shift of focus in his statements on the "productivity" of madness as an effect of power. Finally, we sum up our critique by taking into account the history of the reception of Foucault's writings and ask about their potential significance for the contemporary philosophy and history of psychiatry.

  19. A Novel Visual Data Mining Module for the Geographical Information System gvSIG

    Romel Vázquez-Rodríguez

    2013-01-01

    Full Text Available The exploration of large GIS models containing spatio-temporal information is a challenge. In this paper we propose the integration of scientific visualization (ScVis techniques into geographic information systems (GIS as an alternative for the visual analysis of data. Providing GIS with such tools improves the analysis and understanding of datasets with very low spatial density and allows to find correlations between variables in time and space. In this regard, we present a new visual data mining tool for the GIS gvSIG. This tool has been implemented as a gvSIG module and contains several ScVis techniques for multiparameter data with a wide range of possibilities to explore interactively the data. The developed module is a powerful visual data mining and data visualization tool to obtain knowledge from multiple datasets in time and space. A real case study with meteorological data from Villa Clara province (Cuba is presented, where the implemented visualization techniques were used to analyze the available datasets. Although it is tested with meteorological data, the developed module is of general application in the sense that it can be used in multiple application fields related with Earth Sciences.

  20. Expression of the Arabidopsis Sigma Factor SIG5 Is Photoreceptor and Photosynthesis Controlled

    Marina Mellenthin

    2014-08-01

    Full Text Available Two collections of Arabidopsis GAL4 enhancer trap lines were screened for light-intensity dependent reporter gene activation. Line N9313 was isolated for its strong light-intensity regulation. The T-DNA element trapped distant enhancers of the SIG5 promoter, which drives expression of a sigma factor involved in regulation of chloroplast genes for photosystem II core proteins. The T-DNA insertion 715 bp upstream of the transcription initiation site splits the promoter in a distal and proximal part. Both parts are sensitive to blue and red light and depend on photosynthetic electron transport activity between photosystem II and the plastoquinone pool. The mainblue-light sensitivity is localized within a 196-bp sequence (–887 to –691 bp in the proximal promoter region It is preferentially CRY1 and PHYB controlled. Type-I and type-II phytochromes mediate red-light sensitivity via various promoter elements spread over the proximal and distal upstream region. This work characterizes SIG5 as an anterograde control factor of chloroplast gene expression, which is controlled by chloroplast signals in a retrograde manner.

  1. Implementing an SIG based platform of application and service for city spatial information in Shanghai

    Yu, Bailang; Wu, Jianping

    2006-10-01

    Spatial Information Grid (SIG) is an infrastructure that has the ability to provide the services for spatial information according to users' needs by means of collecting, sharing, organizing and processing the massive distributed spatial information resources. This paper presents the architecture, technologies and implementation of the Shanghai City Spatial Information Application and Service System, a SIG based platform, which is an integrated platform that serves for administration, planning, construction and development of the city. In the System, there are ten categories of spatial information resources, including city planning, land-use, real estate, river system, transportation, municipal facility construction, environment protection, sanitation, urban afforestation and basic geographic information data. In addition, spatial information processing services are offered as a means of GIS Web Services. The resources and services are all distributed in different web-based nodes. A single database is created to store the metadata of all the spatial information. A portal site is published as the main user interface of the System. There are three main functions in the portal site. First, users can search the metadata and consequently acquire the distributed data by using the searching results. Second, some spatial processing web applications that developed with GIS Web Services, such as file format conversion, spatial coordinate transfer, cartographic generalization and spatial analysis etc, are offered to use. Third, GIS Web Services currently available in the System can be searched and new ones can be registered. The System has been working efficiently in Shanghai Government Network since 2005.

  2. Sustainability of State-Level Substance Abuse Prevention Infrastructure After the Completion of the SPF SIG.

    Edwards, Jessica M; Stein-Seroussi, Al; Flewelling, Robert L; Orwin, Robert G; Zhang, Lei

    2015-06-01

    Recent national substance abuse prevention efforts that have been disseminated at the state level have provided fertile ground for addressing the dearth of systematic research on state-level substance abuse prevention infrastructure. The Strategic Prevention Framework State Incentive Grant Program (SPF SIG), a national public health initiative sponsored by the US Substance Abuse and Mental Health Services Administration and its Center for Substance Abuse Prevention, is one such effort, providing an opportunity to examine state-level substance abuse prevention infrastructure across the country. The aims of the SPF SIG initiative include reducing substance abuse and its related problems, as well as enhancing state and local prevention infrastructure and capacity. In this article, we describe the status of state-level substance abuse prevention infrastructure and capacity 1 year after the first 26 funded states ended their projects, based on follow-up interviews with state prevention decision-makers. We found that, in five of the six prevention domains we measured, prevention infrastructure capacity increased during the 12-month period after the grants ended. The evidence for further SPF capacity development even after the conclusion of the grants suggests that states recognized the benefits of using the SPF and took deliberate steps to sustain and enhance the integration of this framework into their state prevention systems. In addition, the findings suggest that state agencies and organizations can benefit from time-limited resources aimed at increasing their capacity and that such efforts can have a lasting impact on measures of state prevention system capacity.

  3. Rancang Bangun Sistem Informasi Geografis (SIG Pemetaan Pemancar Televisi Digital Terestrial di Indonesia

    Isna Nur Mahmud

    2015-03-01

    Full Text Available Perubahan dari sistem televisi analog menjadi sistem televisi digital terestrial di Indonesia tinggal menunggu waktu. Namun masih banyak infrastruktur yang masih perlu dibangun untuk menunjang sistem televisi digital terestrial agar dapat beroperasi dengan baik. Belum meratanya sistem pemancar televisi digital terestrial yang keberadaannya masih terbenturnya undang – undang yang berlaku di negara ini menjadi salah satu permasalahannya. Salah satu solusinya adalah memetakannya dalam sebuah SIG. Pemetaan pemancar tv digital terestrial ini dibuat untuk mempermudah KPI  dalam melakukan identifikasi letak pemancar televisi digital terestrial serta memberikan informasi yang berkaitan dengan daya pemancar, spesifikasi pemancar televisi digital terestrial di Indonesia dalam kondisi offline. Dari pengujian didapatkan hasil antara lain, untuk pengujian black-box, didapatkan hasil yang sesuai dengan fungsionalitas sistem. Untuk nilai MOS, kemudahan menu aplikasi 3.9, kemudahan dlm navigasi aplikasi 4.1, kemudahan dlm menggunakan tools 4.05, penilaian tampilan interface 3.952, penilaian keseluruhan aplikasi SIG 4.hasil SUS yang dilakukan didapatkan nilai 65.71

  4. Conformation-specific anti-Mad2 monoclonal antibodies for the dissection of checkpoint signaling

    Sedgwick, Garry G; Larsen, Marie Sofie Yoo; Lischetti, Tiziana

    2016-01-01

    The spindle assembly checkpoint (SAC) ensures accurate chromosome segregation during mitosis by delaying the activation of the anaphase-promoting complex/cyclosome (APC/C) in response to unattached kinetochores. The Mad2 protein is essential for a functional checkpoint because it binds directly t...

  5. The contaminated Western Hero and other madness during the Atomic Age

    Herzog, Rudolph

    2012-01-01

    The book on mad activities during the Atomic Age includes the following topics: The second most hazardous invention in all time; the red bomb; the story on the tactic nuclear war; the contaminated Western hero or how the bomb came to Alaska; swords into plowshares; the apocalypse machine; flying reactors; how safe is safe?; atomic Australia; nuclear medicine on wrong ways; broken arrows.

  6. An Act of Methodology: A Document in Madness--Writing Ophelia

    Steinnes, Jenny

    2012-01-01

    This paper is an attempt to stage some questions concerning methodology and education, inspired by Ophelia in Shakespeare's "Hamlet" and by Jacques Derrida's poetic philosophical oeuvres. What are at stake are the long traditions of preferences of sanity over madness, friend over enemy, male over female and of clean, unambiguous univocal language…

  7. Automatic Radiometric Normalization of Multitemporal Satellite Imagery with the Iteratively Re-weighted MAD Transformation

    Canty, Morton John; Nielsen, Allan Aasbjerg

    2008-01-01

    A recently proposed method for automatic radiometric normalization of multi- and hyper-spectral imagery based on the invariance property of the Multivariate Alteration Detection (MAD) transformation and orthogonal linear regression is extended by using an iterative re-weighting scheme involving no...

  8. MAD about the Large Magellanic Cloud Preparing for the era of Extremely Large Telescopes

    Fiorentino, G.; Tolstoy, E.; Diolaiti, E.; Valenti, E.; Cignoni, M.; Mackey, A. D.

    We present J, H, K-s photometry from the the Multi conjugate Adaptive optics Demonstrator (MAD), a visitor instrument at the VLT, of a resolved stellar population in a small crowded field in the bar of the Large Magellanic Cloud near the globular cluster NGC 1928. In a total exposure time of 6, 36

  9. pikelet MADS develo S-box opmen genes nt and m maize

    SAM

    ze plant. (B) M e spikelet. Late e floret of female in female ens in the up elet bearing efore, unisex ... e plant MADS members of a ..... and their role in the development and evolution of flowering plants. Mol. .... Classification and phylogeny of.

  10. Eesti parima lühifilmi tegi Madli Lääne

    2007-01-01

    Pimedate Ööde filmifestivali tudengi- ja lühifilmide alafestivali Sleepwalkers parimaks tunnistati Poola tudengi Jan Wagneri lühimängufilm "Porno". Eesti võistluse võitis Madli Lääne dokfilm "Tähelepanu, start!". Parim mängufilm oli Rootsi "Korterikaaslased" (režii Magnus Mork) ja animafilm USA "Erinevad asjaolud"

  11. The Sad, the Mad and the Bad: Co-Existing Discourses of Girlhood

    Brown, Marion

    2011-01-01

    Three significant, prevailing and overlapping narratives of teenage girls have dominated North American popular consciousness since the early 1990s: the sad girl, victimized by male privilege and misogyny of adolescence and beyond; the mad grrrls who rejected this vulnerability through music and media; and the bad girls of much current popular…

  12. Proteomics and SSH analyses of ALA-promoted fruit coloration and evidence for the involvement of a MADS-box gene, MdMADS1

    Xinxin Feng

    2016-11-01

    Full Text Available Skin color is a key quality attribute of fruits and how to improve fruit coloration has long been a major concern. 5-Aminolevulinic acid (ALA, a natural plant growth regulator, can significantly increase anthocyanin accumulation in fruit skin and therefore effectively improve coloration of many fruits, including apple. However, the molecular mechanism how ALA stimulates anthocyanin accumulation in fruit skin remains unknown. Here, we investigated the impact of ALA on apple skin at the protein and mRNA levels. A total of 85 differentially expressed proteins in apple skins between ALA and water treatment (control were identified by complementary gel-based and gel-freeseparation techniques. Most of these differentially expressed proteins were up-regulated by ALA. Function analysis suggested that 87.06% of the ALA-responsive proteins were associated with fruit ripening. To further screen ALA-responsive regulators, we constructed a subtracted cDNA library (tester: ALA treatment; driver: control and obtained 104 differentially expressed unigenes, of which 38 unigenes were indicators for the fruit ripening-related gene. The differentially changed proteins and transcripts did not correspond well at an individual level, but showed similar regulated direction in function at the pathway level. Among the identified fruit ripening-related genes, the expression of MdMADS1, a developmental transcription regulator of fruit ripening, was positively correlated with expression of anthocyanin biosynthetic genes (MdCHS, MdDFR, MdLDOX and MdUFGT in apple skin under ALA treatment. Moreover, overexpression of MdMADS1 enhanced anthocyanin content in transformed apple calli, which was further enhanced by ALA. The anthocyanin content in MdMADS1-silenced calli was less than that in the control with ALA treatment, but higher than that without ALA treatment. These results indicated that MdMADS1 is involved in ALA-induced anthocyanin accumulation. In addition, anthocyanin

  13. SIG-TUR: UMA HERRAMIENTA PARA LA PLANIFICACIÓN, GESTIÓN Y CONTROL DE LOS DESTINOS TURISTICOS

    Ghedin, Leila Marcia; Alves da Silva, Moivan; Duarte Sevalho, Carla Danielle; da Silva Level, Tainah

    2012-01-01

    El Sistema de Información Geográfica Aplicado al Turismo – SIG-Tur es una herramienta tecnológica que optimiza los estudios turísticos desarrollados por los planificadores del sector. En el estudio se buscó evidenciar el instrumento de planificación SIG-Tur, que dispone de funciones de registro, almacenamiento y manipulación de informaciones, además de considerar las dificultades enfrentadas al respecto de la representación de fenómenos espaciales que el turismo abarca. Para el estudio se ado...

  14. Ciclos pelíticos de bahía Interdistributaria del nivel intramareal inferior del grupo Roda-Oroel (sector occidental del paleodelta de Ager, Lérida

    Santiesteban, C.

    1984-12-01

    Full Text Available The deposits of the ancient Ager deltaic system (Lérida, Spain are characterized in some way by the existence of cycles constituted, in a large scale, by alternances of pelites and sandstone bodies. According to geological literature these deposits represent bay marls and stream-mouth-bars, in the eastern-most counterpart. In this area sorne pelitic sections show a pronounced cyclical pattern devoid to repetitions of two terms: A marly bioturbated one, and the other one, constituted by undisturbed delicately laminated clays. Part of the deposits of the "Intramarea1 Inferior" level (''Tramo Superior" of the Roda-Oroel depositional system in the area comprised between La Régola cementery and Ribellera gorge, have been studied taken specially into account the faunistic content, sedimentary structures sequences and lateral relationships of these pelitic cycles in order to characterize them. In that area, three cycles constituted by alternances of marls and clays have been distinguished. As a whole these three cycles are bounded at: the top and the bottom by sanstone bodies that have been interpreted as minor-mouth-bars. The mar1y terms of these cycles are light brown in colour and show a faunistic content that may be ascribed to a lagoon with normal salinity or slight1y hipersaline waters. On the other hand, fauna in the laminated clays is scarce and there is not evidence of bioturbation. The high content of plant remains ad evaporites in these clays suggest that they were developed in a reducing environment (probably anoxic. Boundaries between marls and laminated clays are always net and not erosive. They migth be originated by drastic changes in the physical-chemical characteristics of the sedimentary environment where the were deposited. Taking into account the sedimentary context of these clays, this cyclicity migth be interpreted as the response to the alternance of succesive periods of marine flood and periods of starved waters in a deltaic

  15. Identification and Characterization of the MADS-Box Genes and Their Contribution to Flower Organ in Carnation (Dianthus caryophyllus L.).

    Zhang, Xiaoni; Wang, Qijian; Yang, Shaozong; Lin, Shengnan; Bao, Manzhu; Bendahmane, Mohammed; Wu, Quanshu; Wang, Caiyun; Fu, Xiaopeng

    2018-04-04

    Dianthus is a large genus containing many species with high ornamental economic value. Extensive breeding strategies permitted an exploration of an improvement in the quality of cultivated carnation, particularly in flowers. However, little is known on the molecular mechanisms of flower development in carnation. Here, we report the identification and description of MADS-box genes in carnation ( DcaMADS ) with a focus on those involved in flower development and organ identity determination. In this study, 39 MADS-box genes were identified from the carnation genome and transcriptome by the phylogenetic analysis. These genes were categorized into four subgroups (30 MIKC c , two MIKC*, two Mα, and five Mγ). The MADS-box domain, gene structure, and conserved motif compositions of the carnation MADS genes were analysed. Meanwhile, the expression of DcaMADS genes were significantly different in stems, leaves, and flower buds. Further studies were carried out for exploring the expression of DcaMADS genes in individual flower organs, and some crucial DcaMADS genes correlated with their putative function were validated. Finally, a new expression pattern of DcaMADS genes in flower organs of carnation was provided: sepal (three class E genes and two class A genes), petal (two class B genes, two class E genes, and one SHORT VEGETATIVE PHASE ( SVP )), stamen (two class B genes, two class E genes, and two class C), styles (two class E genes and two class C), and ovary (two class E genes, two class C, one AGAMOUS-LIKE 6 ( AGL6 ), one SEEDSTICK ( STK ), one B sister , one SVP , and one Mα ). This result proposes a model in floral organ identity of carnation and it may be helpful to further explore the molecular mechanism of flower organ identity in carnation.

  16. Identification and Characterization of the MADS-Box Genes and Their Contribution to Flower Organ in Carnation (Dianthus caryophyllus L.

    Xiaoni Zhang

    2018-04-01

    Full Text Available Dianthus is a large genus containing many species with high ornamental economic value. Extensive breeding strategies permitted an exploration of an improvement in the quality of cultivated carnation, particularly in flowers. However, little is known on the molecular mechanisms of flower development in carnation. Here, we report the identification and description of MADS-box genes in carnation (DcaMADS with a focus on those involved in flower development and organ identity determination. In this study, 39 MADS-box genes were identified from the carnation genome and transcriptome by the phylogenetic analysis. These genes were categorized into four subgroups (30 MIKCc, two MIKC*, two Mα, and five Mγ. The MADS-box domain, gene structure, and conserved motif compositions of the carnation MADS genes were analysed. Meanwhile, the expression of DcaMADS genes were significantly different in stems, leaves, and flower buds. Further studies were carried out for exploring the expression of DcaMADS genes in individual flower organs, and some crucial DcaMADS genes correlated with their putative function were validated. Finally, a new expression pattern of DcaMADS genes in flower organs of carnation was provided: sepal (three class E genes and two class A genes, petal (two class B genes, two class E genes, and one SHORT VEGETATIVE PHASE (SVP, stamen (two class B genes, two class E genes, and two class C, styles (two class E genes and two class C, and ovary (two class E genes, two class C, one AGAMOUS-LIKE 6 (AGL6, one SEEDSTICK (STK, one B sister, one SVP, and one Mα. This result proposes a model in floral organ identity of carnation and it may be helpful to further explore the molecular mechanism of flower organ identity in carnation.

  17. Sequence motifs in MADS transcription factors responsible for specificity and diversification of protein-protein interaction.

    Aalt D J van Dijk

    Full Text Available Protein sequences encompass tertiary structures and contain information about specific molecular interactions, which in turn determine biological functions of proteins. Knowledge about how protein sequences define interaction specificity is largely missing, in particular for paralogous protein families with high sequence similarity, such as the plant MADS domain transcription factor family. In comparison to the situation in mammalian species, this important family of transcription regulators has expanded enormously in plant species and contains over 100 members in the model plant species Arabidopsis thaliana. Here, we provide insight into the mechanisms that determine protein-protein interaction specificity for the Arabidopsis MADS domain transcription factor family, using an integrated computational and experimental approach. Plant MADS proteins have highly similar amino acid sequences, but their dimerization patterns vary substantially. Our computational analysis uncovered small sequence regions that explain observed differences in dimerization patterns with reasonable accuracy. Furthermore, we show the usefulness of the method for prediction of MADS domain transcription factor interaction networks in other plant species. Introduction of mutations in the predicted interaction motifs demonstrated that single amino acid mutations can have a large effect and lead to loss or gain of specific interactions. In addition, various performed bioinformatics analyses shed light on the way evolution has shaped MADS domain transcription factor interaction specificity. Identified protein-protein interaction motifs appeared to be strongly conserved among orthologs, indicating their evolutionary importance. We also provide evidence that mutations in these motifs can be a source for sub- or neo-functionalization. The analyses presented here take us a step forward in understanding protein-protein interactions and the interplay between protein sequences and

  18. Using Social Impact Games (SIGS) to Support Constructivist Learning: Creating a Foundation for Effective Use in the Secondary Social Studies Education

    Ray, Beverly; Faure, Caroline; Kelle, Fay

    2013-01-01

    This paper examines how Social Impact Games (SIGs) can provide important instructional support in secondary social studies classrooms. When used within the framework of the constructivist teaching philosophy and teaching methods, as recommended by the NCSS (2010), SIGs have the potential to hone critical thinking, collaboration, and problem…

  19. The immunogenic SigA enterotoxin of Shigella flexneri 2a binds to HEp-2 cells and induces fodrin redistribution in intoxicated epithelial cells.

    Keith Al-Hasani

    Full Text Available BACKGROUND: We have previously shown that the enterotoxin SigA which resides on the she pathogenicity island (PAI of S. flexneri 2a is an autonomously secreted serine protease capable of degrading casein. We have also demonstrated that SigA is cytopathic for HEp-2 cells and plays a role in the intestinal fluid accumulation associated with S. flexneri infections. METHODS/PRINCIPAL FINDINGS: In this work we show that SigA binds specifically to HEp-2 cells and degrades recombinant human alphaII spectrin (alpha-fodrin in vitro, suggesting that the cytotoxic and enterotoxic effects mediated by SigA are likely associated with the degradation of epithelial fodrin. Consistent with our data, this study also demonstrates that SigA cleaves intracellular fodrin in situ, causing its redistribution within cells. These results strongly implicate SigA in altering the cytoskeleton during the pathogenesis of shigellosis. On the basis of these findings, cleavage of fodrin is a novel mechanism of cellular intoxication for a Shigella toxin. Furthermore, information regarding immunogenicity to SigA in infected patients is lacking. We studied the immune response of SigA from day 28 post-challenge serum of one volunteer from S. flexneri 2a challenge studies. Our results demonstrate that SigA is immunogenic following infection with S. flexneri 2a. CONCLUSIONS: This work shows that SigA binds to epithelial HEp-2 cells as well as being able to induce fodrin degradation in vitro and in situ, further extending its documented role in the pathogenesis of Shigella infections.

  20. Selenide isotope generator for the Galileo Mission: SIG thermal insulation evaluaion tests

    1979-06-01

    Since the SIG program required the use of very high performance thermal insulation materials in rather severe thermal and environmental conditions, a thorough screening and testing program was performed. Several types of materials were included in the preliminary survey. Most promising were oxide and carbonaceous fibrous insulations, oxide and carbonaceous foamed materials, and multilayer materials with both powder and cloth spacers. The latter were only viable for the vacuum option. In all, over one hundred materials from more than sixty manufacturers were evaluated from literature and manufacturers' data. The list was pared to eighteen candidates in seven basic types, i.e., fibrous microporous SiO 2 , fibrous SiO 2 /Al 2 O 3 , fibrous ZrO 2 , fibrous carbon, foamed SiO 2 , foamed carbon, and multilayer. Test results are presented

  1. Observation spatiale et SIG: des outils pour cartographier les zones sensibles aux mouvements de terrain

    Jean-Yves SCANVIC

    1994-12-01

    Full Text Available Les niveaux de sensibilité des sols aux mouvements de terrain ont été cartographiés dans différents bassins de risques en Bolivie, en Colombie et à Taïwan, selon une méthodologie développée au BRGM et fondée en partie sur l’extraction visuelle et numérique d’informations contenues dans les données de télédétection spatiale stéréoscopiques et leur gestion-valorisation dans un SIG. Ces cartes font apparaître l’intérêt de l’imagerie Spot pour la gestion du risque naturel.

  2. Anti-dentine Salivary SIgA in young adults with a history of dental trauma in deciduous teeth

    Gabriela Fleury SEIXAS

    2015-01-01

    Full Text Available Anti-dentin autoantibodies are associated with inflammatory root resorption in permanent teeth and are modulated by dental trauma and orthodontic force. However, it is not known whether deciduous tooth trauma can stimulate the development of a humoral immune response against dentin. The aim of this study was to evaluate the levels of salivary SIgA reactivity against human dentin extract in young adults with a history of trauma in the primary dentition. A sample of 78 patients, aged 18 to 25, who had completed an early childhood (0 to 5 years old caries prevention program years earlier at the Universidade Estadual de LondrinaPediatric Clinic, underwent radiographic examination and salivary sampling. Anti-dentin SIgA levels were analyzed by immunoenzymatic assay and Western blotting. Although dental trauma to deciduous teeth had occurred in 34 (43.6% of the patients, no differences in SIgA levels were detected between individuals who had experienced trauma and those who had not (p > 0.05. Multivariate regression analysis showed no association between dental trauma and SIgA levels (p > 0.05. Patients with a history of deciduous trauma presented low levels of anti-dentin antibodies, associated with orthodontic root resorption (p

  3. Clinical efficacy and safety of surface imaging guided radiosurgery (SIG-RS) in the treatment of benign skull base tumors.

    Lau, Steven K M; Patel, Kunal; Kim, Teddy; Knipprath, Erik; Kim, Gwe-Ya; Cerviño, Laura I; Lawson, Joshua D; Murphy, Kevin T; Sanghvi, Parag; Carter, Bob S; Chen, Clark C

    2017-04-01

    Frameless, surface imaging guided radiosurgery (SIG-RS) is a novel platform for stereotactic radiosurgery (SRS) wherein patient positioning is monitored in real-time through infra-red camera tracking of facial topography. Here we describe our initial clinical experience with SIG-RS for the treatment of benign neoplasms of the skull base. We identified 48 patients with benign skull base tumors consecutively treated with SIG-RS at a single institution between 2009 and 2011. Patients were diagnosed with meningioma (n = 22), vestibular schwannoma (n = 20), or nonfunctional pituitary adenoma (n = 6). Local control and treatment-related toxicity were retrospectively assessed. Median follow-up was 65 months (range 61-72 months). Prescription doses were 12-13 Gy in a single fraction (n = 18), 8 Gy × 3 fractions (n = 6), and 5 Gy × 5 fractions (n = 24). Actuarial tumor control rate at 5 years was 98%. No grade ≥3 treatment-related toxicity was observed. Grade ≤2 toxicity was associated with symptomatic lesions (p = 0.049) and single fraction treatment (p = 0.005). SIG-RS for benign skull base tumors produces clinical outcomes comparable to conventional frame-based SRS techniques while enhancing patient comfort.

  4. Modelo basado en SIG para el estudio de la mortalidad por enfermedad cerebro-vascular

    Carlos Alberto Tangarife Villa

    2013-06-01

    Full Text Available En los últimos 15 años el estudio para una de las tres primeras causas de muerte a nivel mundial –enfermedad cerebrovascular (ECV– se caracteriza por utilizar el enfoque epidemiológico tradicional y sus técnicas de análisis estadístico bivariado y multivariado orientadas a identificar su composición según variables de persona y explorar relaciones con sus determinantes pero sin impactar significativamente su ocurrencia, sin embargo, en el presente artículo se identifica como alternativa de análisis la modelación espacio-temporal con SIG a niveles de desagregación detallados; en este sentido el análisis comparativo de los modelos utilizados en los estudios conocidos, desvela el uso de datos y técnicas de análisis espacial básicas para identificar patrones como los evidenciados en los cinturones de diabetes y ECV en USA o el clúster colombiano de enfermedades del aparato circulatorio identificado en la región andina que sugiere un comportamiento similar para la ECV. El panorama anterior demanda la combinación de enfoques con énfasis en sus técnicas de análisis avanzadas –análisis multi-criterio, kriging, interpolación, correlación y regresión espacial– soportadas con SIG y por lo tanto, como contribución final se propone un modelo integrado dirigido a impactar positivamente la mortalidad por ECV.

  5. Pemilihan Lokasi Kawasan Konservasi Mangrove dengan Pendekatan SIG Partisipatif di Wilayah Pantai Kabupaten Demak

    Elida Nurrohmah

    2016-10-01

    Full Text Available Penentuan lokasi kawasan konservasi berkaitan dengan keputusan penggunaan lahan, yang melibatkan banyak aktor dan faktor. Analisis yang bersifat multifaktor dapat diselesaikan dengan memanfaatkan Sistem Informasi Geografis (SIG, namun penilaiannya hanya berdasarkan pada penilaian tunggal dari seseorang ataupun suatu institusi. Penelitian ini berusaha untuk mengintegrasikan analisis yang bersifat multiaktor dan multifaktor melalui analisis multikriteria dengan SIG berbasis partisipasi masyarakat untuk memilih lokasi kawasan konservasi mangrove. Kriteria yang dipertimbangkan dalam pemilihan lokasi ini meliputi aspek (i fisik, (ii sosial, (iii pembiayaan, dan (iv ancaman. Kriteria aspek fisik disusun berdasarkan hasil analisis kesesuaian lahan untuk mangrove. Kriteria aspek sosial, pembiayaan, dan ancaman disusun berdasarkan data sosial kependudukan yang diperoleh melalui studi literatur. Partisipasi masyarakat dalam proses pemilihan lokasi ini terletak pada penentuan bobot kriteria melalui perbandingan kriteria berpasangan dan dianalisis dengan teknik Analytical Hierarchial Process (AHP, serta penggalian informasi lokal melalui pemetaan partisipatif. Bobot kriteria setiap pemangku kepentingan diintegrasikan dengan data spasial melalui operasi perkalian raster untuk menghasilkan lokasi kawasan konservasi mangrove terpilih kombinasi seluruh pemangku kepentingan. Hasil analisis kesesuaian lahan menunjukkan bahwa lahan dengan kelas sesuai untuk mangrove seluas 11.564 ha (94,8% dari luas daerah penelitian dan sesuai marginal seluas 629 ha. Pemangku kepentingan pada tingkat lokal (kelompok mangrove, petani tambak, dan tokoh masyarakat lebih memprioritaskan aspek sosial dibandingkan aspek fisik, pembiayaan, dan ancaman; sedangkan pemerintah daerah lebih memprioritaskan aspek pembiayaan. Dengan prioritas tersebut, lokasi kawasan konservasi mangrove  paling memenuhi prioritas pemangku kepentingan (prioritas I didapati seluas 51,7 ha; terletak di wilayah

  6. Secretory IgA (SIgA: designed for antimicrobial defence

    Per eBrandtzaeg

    2013-08-01

    Full Text Available Prevention of infections by vaccination remains a compelling goal to improve public health. Mucosal vaccines would make immunization procedures easier, be better suited for mass administration, and most efficiently induce immune exclusion -- a term coined for non-inflammatory antibody shielding of internal body surfaces, mediated principally by secretory immunoglobulin A (SIgA. The exported antibodies are polymeric, mainly IgA dimers (pIgA, produced by local plasma cells stimulated by antigens that target the mucosae. SIgA was early shown to be complexed with an epithelial glycoprotein -- the secretory component (SC. A common SC-dependent transport mechanism for pIgA and pentameric IgM was then proposed, implying that membrane SC acts as a receptor, now usually called the polymeric Ig receptor (pIgR. From the basolateral surface, pIg-pIgR complexes are taken up by endocytosis and then extruded into the lumen after apical cleavage of the receptor -- bound SC having stabilizing and innate functions in the secretory antibodies. Mice deficient for pIgR show that this is the only receptor responsible for epithelial export of IgA and IgM. These knockout mice show a variety of defects in their mucosal defensce and changes in their intestinal microbiota. In the gut, induction of B cells occurs in gut-associated lymphoid tissue (GALT, particularly the Peyer’s patches and isolated lymphoid follicles, but also in mesenteric lymph nodes. Plasma cell differentiation is accomplished in the lamina propria to which the activated memory/effector B cells home. The airways also receive such cells from nasopharynx-associated lymphoid tissue (NALT but by different homing receptors. This compartmentalization is a challenge for mucosal vaccination, as are the mechanisms used by the mucosal immune system to discriminate between commensal symbionts (mutualism, pathobionts and overt pathogens (elimination.

  7. ChromaSig: a probabilistic approach to finding common chromatin signatures in the human genome.

    Gary Hon

    2008-10-01

    Full Text Available Computational methods to identify functional genomic elements using genetic information have been very successful in determining gene structure and in identifying a handful of cis-regulatory elements. But the vast majority of regulatory elements have yet to be discovered, and it has become increasingly apparent that their discovery will not come from using genetic information alone. Recently, high-throughput technologies have enabled the creation of information-rich epigenetic maps, most notably for histone modifications. However, tools that search for functional elements using this epigenetic information have been lacking. Here, we describe an unsupervised learning method called ChromaSig to find, in an unbiased fashion, commonly occurring chromatin signatures in both tiling microarray and sequencing data. Applying this algorithm to nine chromatin marks across a 1% sampling of the human genome in HeLa cells, we recover eight clusters of distinct chromatin signatures, five of which correspond to known patterns associated with transcriptional promoters and enhancers. Interestingly, we observe that the distinct chromatin signatures found at enhancers mark distinct functional classes of enhancers in terms of transcription factor and coactivator binding. In addition, we identify three clusters of novel chromatin signatures that contain evolutionarily conserved sequences and potential cis-regulatory elements. Applying ChromaSig to a panel of 21 chromatin marks mapped genomewide by ChIP-Seq reveals 16 classes of genomic elements marked by distinct chromatin signatures. Interestingly, four classes containing enrichment for repressive histone modifications appear to be locally heterochromatic sites and are enriched in quickly evolving regions of the genome. The utility of this approach in uncovering novel, functionally significant genomic elements will aid future efforts of genome annotation via chromatin modifications.

  8. Multivariate alteration detection (MAD) in multispectral, bi-temporal image data: A new approach to change detction studies

    Nielsen, Allan Aasbjerg; Conradsen, Knut

    This paper introduces a new orthogonal transformation, the multivariate alteration detection (MAD) transformation, based on an established multivariate statistical technique canonical correlation analysis. The theory for canonical correlation analysis is sketched and a result necessary...... for the definition of the MAD transformation is proven. As opposed to traditional univariate change detection schemes our scheme transforms two sets of multivariate observations (e.g. two multispectral satellite images covering the same geographical area acquired at different points in time) into a difference...... between two linear combinations of the original variables explaining maximal change (i.e. the difference explaining maximal variance) in all variables simultaneously. The MAD transformation is invariant to linear scaling. The MAD transformation can be used iteratively. First, it can be used to detect...

  9. Casein kinase II is required for the spindle assembly checkpoint by regulating Mad2p in fission yeast

    Shimada, Midori [Department of Biochemistry and Cell Biology, Graduate School of Medicine, Nagoya City University, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601 (Japan); Yamamoto, Ayumu [Department of Chemistry, Shizuoka University, 836 Ohya, Suruga-ku, Sizuoka 422-8529 (Japan); Murakami-Tonami, Yuko; Nakanishi, Makoto; Yoshida, Takashi [Department of Biochemistry and Cell Biology, Graduate School of Medicine, Nagoya City University, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601 (Japan); Aiba, Hirofumi [Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Chikusa-ku, Nagoya 464-8601 (Japan); Murakami, Hiroshi, E-mail: hmura@med.nagoya-cu.ac.jp [Department of Biochemistry and Cell Biology, Graduate School of Medicine, Nagoya City University, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601 (Japan)

    2009-10-23

    The spindle checkpoint is a surveillance mechanism that ensures the fidelity of chromosome segregation in mitosis. Here we show that fission yeast casein kinase II (CK2) is required for this checkpoint function. In the CK2 mutants mitosis occurs in the presence of a spindle defect, and the spindle checkpoint protein Mad2p fails to localize to unattached kinetochores. The CK2 mutants are sensitive to the microtubule depolymerising drug thiabendazole, which is counteracted by ectopic expression of mad2{sup +}. The level of Mad2p is low in the CK2 mutants. These results suggest that CK2 has a role in the spindle checkpoint by regulating Mad2p.

  10. Analysis of MADS-Box Gene Family Reveals Conservation in Floral Organ ABCDE Model of Moso Bamboo (Phyllostachys edulis

    Zhanchao Cheng

    2017-05-01

    Full Text Available Mini chromosome maintenance 1, agamous, deficiens, and serum response factor (MADS-box genes are transcription factors which play fundamental roles in flower development and regulation of floral organ identity. However, till date, identification and functions of MADS-box genes remain largely unclear in Phyllostachys edulis. In view of this, we performed a whole-genome survey and identified 34 MADS-box genes in P. edulis, and based on phylogeny, they were classified as MIKCC, MIKC∗, Mα, and Mβ. The detailed analysis about gene structure and motifs, phylogenetic classification, comparison of gene divergence and duplication are provided. Interestingly, expression patterns for most genes were found similar to those of Arabidopsis and rice, indicating that the well-established ABCDE model can be applied to P. edulis. Moreover, we overexpressed PheMADS15, an AP1-like gene, in Arabidopsis, and found that the transgenic plants have early flowering phenotype, suggesting that PheMADS15 might be a regulator of flowering transition in P. edulis. Taken together, this study provides not only insightful comprehension but also useful information for understanding the functions of MADS-box genes in P. edulis.

  11. MADNESS AND METHOD: THE SYSTEM OF DOCTOR TARR AND PROFESSOR FETHER

    Luciane Alves Santos

    2014-12-01

    Full Text Available The present study aims to discuss the theme of madness, comparing the narrator’s perspective in “The System of Doctor Tarr and Professor Fether” (1845 and another short stories by Edgar Allan Poe. The analysis illustrates that science in the 19th century aims to remove the fear of the unknown, dominating the natural world and its laws; and that fiction, the presumptive opposite, represents an extension of the same logic under another name. Some writers denied any obligation of loyalty to scientific rationalism and tried to decipher this problem through the dream and the supernatural. Fantastic works of Gothic fiction – Hoffmann’s novels and some of Poe’s stories – illuminate these points. Poe reinterprets the Gothic in the Victorian Age, with an unnamed and often unreliable narrator that insists on his rationality. The comic and grotesque horror result of the psychology of his characters often descended into madness

  12. VISITING THE MUSEUM OF MADNESS: THE EXPERIENCE OF LEARNING ABOUT THE PSYCHIATRIC REFORM

    Nadja Cristiane Lappann Botti

    2006-04-01

    Full Text Available ABSTRACT: The Museum of Madness in the landscape of the Minas Gerais State is a place where art, history and memory disclose the differences about the Psychiatry approach since the beginning of the last century till nowadays. A qualitative study with 39 nursing undergraduate students was developed, aiming to identify the meaning of the visit to the Museum of Madness. The methodological referential was the Collective Subject Discourse and the theoretical the Brazilian Psychiatric Reform. The data shows the meaning of the visit as potential experience of learning of the dimensions of the Psychiatric Reform when leads the student to question the practice and to know of Psychiatry in the asylum perspective. KEY WORDS: Nursing Education; Mental Health; Health Care Reform.

  13. Mediations on Emergent Occasions: Mad Men, Donald Draper and Frank O’Hara

    Kate Lilley

    2012-09-01

    Full Text Available Frank O’Hara’s 1957 poetry collection, Meditations in an Emergency, features in Season Two of Mad Men (2008 as a talismanic phrase and object. Pressed into service as Matthew Weiner’s valentine to his returning viewers, the circulation and citation of the book across the season, through different diegetic and extradiegetic levels, aligns poetry, advertising and quality serial television drama as textual modes intent, above all, on creating attachment through feeling. O’Hara’s book is a crucial link in a series of metonymic relays, chain effects and affects, which underwrite Mad Men’s citational poetics to assert its own cultural authority, and the mediating power of television.

  14. Mad og måltider bidrager til dannelse og læring

    Jacobsen, Mikkel

    2017-01-01

    Vi lever i en kultur der på mange måder knytter vores identitet til mad, fx i forhold til hvad vi poster på sociale medier, om vi køber økologi, stenaldermad, er tykke eller tynde, om vi går op i dyrevelfærd, miljø, osv. Elementer der er med til at skabe vores identitet gennem de valg vi træffer...

  15. Reason and madness in the structure of the Cartesian cogito. Three interpretations of Rene Descartes' Meditation

    Karachevtseva Larysа Mykolaivna

    2013-06-01

    Full Text Available The article analyses the polemics between Michel Foucault and Jacques Derrida that took place in the sixties of the 20th century. This polemics was dedicated to the elucidation of the role of reason and madness within Cartesian radical doubt – that thought experiment maintained the formula ego cogito ergo sum. The article also examines Emmanuel Levinas's interpretation of Descartes' idea of the infinite. It is shown that the idea of the infinite constitutes cogito.

  16. Evaluation of torque loss value of MAD/MAM zirconia abutments with prefabricated titanium abutments

    Marzieh Alikhasi; Roshanak Baghaie; Nasim khosronejad

    2013-01-01

    Background and Aims: In response to esthetic demand of patients, ceramic abutments have been developed. Despite esthetic of zirconia abutments, machining accuracy of these abutments has always been a question. Any misfit in the abutment-implant interface connection can lead to detorque and screw loosening. The aim of this study was to compare torque loss value of manually aided design/manually aided manufacture (MAD/MAM) zirconia abutments with prefabricated titanium abutments. Materials and ...

  17. Der Sinn des Wahns : Der Mad Scientist und die unmögliche Wissenschaft

    Keller, Felix

    2004-01-01

    Der Text untersucht die Herkunft des verrückten Wissenschaftlers, des Mad Scientist, so wie er in den populären Kultur zelebriert wird. Es wird die Auffassung vertreten, dass der Ursprung der Figur im romantischen Mythos des Genies liegt. Doch wie und weshalb ist es dann zu dieser seltsamen Mutation zum verrückten Wissenschaftler gekommen? Und was berichtet diese Figur über die Wahrnehmung von Wissenschaft?

  18. For a minor art: resonances between art, clinical practice and madness nowadays

    Elizabeth Maria Freire de Araújo Lima

    2007-01-01

    Full Text Available We discuss the changes that were brought about in Brazil in the 20th century related to the acceptance of works of art produced in clinics or, in any way, other than those conventionally accepted by the artistic community. The enlargement of this field, now including dissenting works of art, seems to indicate a change in contemporary sensibility therefore shifting the relationships between art, clinical practice and madness itself.

  19. A MAD Explanation for the Correlation between Bulk Lorentz Factor and Minimum Variability Timescale

    Lloyd-Ronning, Nicole; Lei, Wei-hua; Xie, Wei

    2018-04-01

    We offer an explanation for the anti-correlation between the minimum variability timescale (MTS) in the prompt emission light curve of gamma-ray bursts (GRBs) and the estimated bulk Lorentz factor of these GRBs, in the context of a magnetically arrested disk (MAD) model. In particular, we show that previously derived limits on the maximum available energy per baryon in a Blandford-Znajek jet leads to a relationship between the characteristic MAD timescale in GRBs and the maximum bulk Lorentz factor: tMAD∝Γ-6, somewhat steeper than (although within the error bars of) the fitted relationship found in the GRB data. Similarly, the MAD model also naturally accounts for the observed anti-correlation between MTS and gamma-ray luminosity L in the GRB data, and we estimate the accretion rates of the GRB disk (given these luminosities) in the context of this model. Both of these correlations (MTS - Γ and MTS - L) are also observed in the AGN data, and we discuss the implications of our results in the context of both GRB and blazar systems.

  20. FlyMAD: rapid thermogenetic control of neuronal activity in freely walking Drosophila.

    Bath, Daniel E; Stowers, John R; Hörmann, Dorothea; Poehlmann, Andreas; Dickson, Barry J; Straw, Andrew D

    2014-07-01

    Rapidly and selectively modulating the activity of defined neurons in unrestrained animals is a powerful approach in investigating the circuit mechanisms that shape behavior. In Drosophila melanogaster, temperature-sensitive silencers and activators are widely used to control the activities of genetically defined neuronal cell types. A limitation of these thermogenetic approaches, however, has been their poor temporal resolution. Here we introduce FlyMAD (the fly mind-altering device), which allows thermogenetic silencing or activation within seconds or even fractions of a second. Using computer vision, FlyMAD targets an infrared laser to freely walking flies. As a proof of principle, we demonstrated the rapid silencing and activation of neurons involved in locomotion, vision and courtship. The spatial resolution of the focused beam enabled preferential targeting of neurons in the brain or ventral nerve cord. Moreover, the high temporal resolution of FlyMAD allowed us to discover distinct timing relationships for two neuronal cell types previously linked to courtship song.

  1. ["I am but mad north-north-west"--Hamlet's portrayed delusion].

    Schulte Herbrüggen, H

    1996-01-01

    Whereas science refers to the real world existing independently and conditioned by cause and effect, the world of literature is fictitious, created by the artist in our imagination by means of language, an artefact conditioned by aesthetic laws, a world sui generis. Accordingly, Hamlet is no person, but a literary figure, doing, saying, thinking and feeling only what the poet dictated him word for word. The essential difference between the two worlds is often overlooked. That "blind spot" has a long-standing tradition in European intellectual history and goes back i.a. to the German "Hamlet experience" in the eighteenth, the "Hamlet fever" and the felt spiritual kinship (Seelenverwandtschaft) in the nineteenth century. Teleological literary criticism, centering around Hamlet's "character" and isolating his psychologically evaluated monologues (e.g. Bradley), refrained from Hamlet's fictionality and role-play and led to blurring beyond recognition the boundaries between real person and literary figure (e.g. Freud, Jones) and assisted in reducing a dramatic role to a medical case history. Speaking of Hamlet, one has to start from Shakespeare's text, our subject matter. A dramatic play being a plot turned into dialogue, the poet's vocabulary used (but indirectly also the vocabulary not used) is particularly informative. When referring to Hamlet's "antic disposition", Shakespeare uses a wide range of over 20 different terms, the most frequented being mad/madness (44 times). Evidence of primary importance are the five occasions after the apparition of his father's ghost, when Hamlet speaks of hist "madness" as an assumed role. In Act I "madness occurs first as a mere possibility when Hamlet informs his friends, he might "put an antic disposition on"; in Act II vis-a-vis Rosencrantz and Guildenstern ("I am but mad north-north-west") it is his deliberate action under certain conditions; in Act III it occurs thrice, first in his declaration of intent ("They are coming to

  2. Patterns of MADS-box gene expression mark flower-type development in Gerbera hybrida (Asteraceae

    Teeri Teemu H

    2006-06-01

    Full Text Available Abstract Background The inflorescence of the cut-flower crop Gerbera hybrida (Asteraceae consists of two principal flower types, ray and disc, which form a tightly packed head, or capitulum. Despite great interest in plant morphological evolution and the tractability of the gerbera system, very little is known regarding genetic mechanisms involved in flower type specification. Here, we provide comparative staging of ray and disc flower development and microarray screening for differentially expressed genes, accomplished via microdissection of hundreds of coordinately developing flower primordia. Results Using a 9K gerbera cDNA microarray we identified a number of genes with putative specificity to individual flower types. Intrestingly, several of these encode homologs of MADS-box transcription factors otherwise known to regulate flower organ development. From these and previously obtained data, we hypothesize the functions and protein-protein interactions of several gerbera MADS-box factors. Conclusion Our RNA expression results suggest that flower-type specific MADS protein complexes may play a central role in differential development of ray and disc flowers across the gerbera capitulum, and that some commonality is shared with known protein functions in floral organ determination. These findings support the intriguing conjecture that the gerbera flowering head is more than a mere floral analog at the level of gene regulation.

  3. Evaluation of torque loss value of MAD/MAM zirconia abutments with prefabricated titanium abutments

    Marzieh Alikhasi

    2013-04-01

    Full Text Available Background and Aims: In response to esthetic demand of patients, ceramic abutments have been developed. Despite esthetic of zirconia abutments, machining accuracy of these abutments has always been a question. Any misfit in the abutment-implant interface connection can lead to detorque and screw loosening. The aim of this study was to compare torque loss value of manually aided design/manually aided manufacture (MAD/MAM zirconia abutments with prefabricated titanium abutments. Materials and Methods: Seven titanium abutments (Branemark RP, Easy abutment and seven copy milled abutments which were duplicated from the prefabricated Zirkonzhan (ZirkonZahn, Sand in Taufers, Italy were prepared. After sintering process of zirconia abutment, all abutments were fastened with a torque screw under 35 Ncm. Detorque measurements were performed per group pushing the reverse button of the Torque controller soon after screw tightening with values registered. The mean torque loss were calculated and compared using Student's t test. Results: The mean of torque loss was 12.71 Ncm with standard deviation of 1.70 for prefabricated titanium abutments and 15.50 Ncm with standard deviation of 4.67 for MAD-MAM abutments. The difference between the two groups was not statistically significant (P=0.23. Conclusion: Within the limitation of this study, MAD-MAM ceramic abutments could maintain the applied torque comparing to the prefabricated abutments.

  4. Dual personality of Mad1: regulation of nuclear import by a spindle assembly checkpoint protein.

    Cairo, Lucas V; Ptak, Christopher; Wozniak, Richard W

    2013-01-01

    Nuclear transport is a dynamic process that can be modulated in response to changes in cellular physiology. We recently reported that the transport activity of yeast nuclear pore complexes (NPCs) is altered in response to kinetochore-microtubule (KT-MT) interaction defects. Specifically, KT detachment from MTs activates a signaling pathway that prevents the nuclear import of cargos by the nuclear transport factor Kap121p. This loss of Kap121p-mediated import is thought to influence the nuclear environment, including the phosphorylation state of nuclear proteins. A key regulator of this process is the spindle assembly checkpoint protein Mad1p. In response to unattached KTs, Mad1p dynamically cycles between NPCs and KTs. This cycling appears to induce NPC molecular rearrangements that prevent the nuclear import of Kap121p-cargo complexes. Here, we discuss the underlying mechanisms and the physiological relevance of Mad1p cycling and the inhibition of Kap121p-mediated nuclear import, focusing on outstanding questions within the pathway.

  5. The social structure of the medical model of madness and the physician's role.

    Fernandez, G

    1981-08-01

    In this paper the origins of the medical model of madness are traced in the sociohistorical context of institutional and professional development. The paper establishes the emergence of the three primary conditions necessary for the medical model to exist: (a) the view that madness is a separate ontological reality which can be differentiated from the insane person; (b) the concept that insane people do not have a completely free will and therefore cannot be held responsible for their actions; and (c) the creation of specific criteria to classify the disease into empirically derived nosologies. These conditions and their acceptance as an explanatory paradigm of insanity result from the political economy of the late Middle Ages and are reflected in the institutional arrangement for insane persons of the 17th and 18th centuries. Finally, the role of the physician-psychiatrist is explained in terms of an ability to (a) serve as a technician for the new political forces, and (b) dislodge the moral entrepreneurs and become the only profession able to offer a proper scientific and secular treatment of madness. The psychiatrist is presented as a by-product of the dominance of the medical model rather than as the agent who created it.

  6. The Duty of the ANARAP-MAD for the Development of Radiation Protection in Madagascar

    Ratovonjanahary, F.

    2010-01-01

    Association NAtionale de Radio Protection-MADagascar (ANARAP-MAD), to develop the radiation protection in Madagascar; to promote the radiation protection in Madagascar; to set up links with international institutions dealing with the same objectives (I.A.E.A., W.H.O., etc...); to care for the enforcement of the regulation in radiation protection; to propose improvements and amendments; to find solutions to problems relevant to the enforcement of the regulation; to inform and to train in the field of radiation protection; to contribute in the development of the science, all activities carried out by the association are based on the existing legislation in the country. Despite the insufficiency of financial support, the ANARAP-MAD had always made an effort to carry out activities for the development of radiation protection infrastructure in Madagascar. The main problem is also that the regulatory Authority is not yet operational. However, the ANARAP-MAD, with the support of the Madagascar-INSTN, has the challenge to effectively promoting radiation protection in Madagascar

  7. Genome-wide characterization of the MADS-box gene family in radish (Raphanus sativus L. and assessment of its roles in flowering and floral organogenesis

    Chao Li

    2016-09-01

    Full Text Available The MADS-box gene family is an important transcription factor (TF family that is involved in various aspects of plant growth and development, especially flowering time and floral organogenesis. Although it has been reported in many plant species, the systematic identification and characterization of MADS-box TF family is still limited in radish (Raphanus sativus L.. In the present study, a comprehensive analysis of MADS-box genes was performed, and a total of 144 MADS-box family members were identified from the whole radish genome. Meanwhile, a detailed list of MADS-box genes from other 28 plant species was also investigated. Through the phylogenetic analysis between radish and Arabidopsis thaliana, all the RsMADS genes were classified into two groups including 68 type I (31 Mα, 12 Mβ and 25Mγ and 76 type II (70 MIKCC and 6 MIKC*. Among them, 41 (28.47% RsMADS genes were located in nine linkage groups of radish from R1 to R9. Moreover, the homologous MADS-box gene pairs were identified among radish, A. thaliana, Chinese cabbage and rice. Additionally, the expression profiles of RsMADS genes were systematically investigated in different tissues and growth stages. Furthermore, quantitative real-time PCR analysis was employed to validate expression patterns of some crucial RsMADS genes. These results could provide a valuable resource to explore the potential functions of RsMADS genes in radish, and facilitate dissecting MADS-box gene-mediated molecular mechanisms underlying flowering and floral organogenesis in root vegetable crops.

  8. Clinical and laboratory findings in mad honey poisoning: a single center experience.

    Yaylaci, S; Kocayigit, I; Aydin, E; Osken, A; Genc, A B; Cakar, M A; Tamer, A

    2014-01-01

    This study is aimed at analyzing the demographic and clinical characteristics, as well as the hematological-biochemical parameters of patients who admitted to the hospital with the diagnosis of mad honey poisoning. A total of 16 patients who were admitted with mad honey intoxication symptoms and treated in Emergency Department of Sakarya Education and Research Hospital between January 2009 and December 2012 were included in the study. Demographic and clinical characteristics of the patients and hematological, biochemical parameters were obtained from hospital records. Heart rate, systolic and diastolic blood pressure on admission and at discharge were obtained retrospectively. Sixteen patients (10 males and 6 females, mean age 58.5 ± 10 years, range between 41 and 79) were included in our study. Heart rate was 42 ± 6 beats/min, systolic blood pressure was 73 ± 19 mmHg, and diastolic blood pressure was 45 ± 17 mmHg on admission. In the evaluation of the patients' heart rhythms on admission to the emergency room, nine (56.3%) patients had sinus bradycardia, three (18.8%) patients had nodal rhythm, two (12.5%) patients had first degree atrioventricular block, and two (12.5%) patients had atrial fibrillation. Atropine 1.1 ± 0.4 mg and saline 1125 ± 465 ml were used to treat patients. Patients were discharged with a stable condition after an average 27.7 ± 7.2 h of follow-up. Heart rate was 75 ± 8 beats/min, systolic blood pressure was 132 ± 7 mmHg, and diastolic blood pressure was 82 ± 6 mmHg at discharge. Mortality was not observed. Hematological and biochemical parameters measured at the time of admission were within normal ranges. Mad honey poisoning should be considered in previously healthy patients with unexplained symptoms of bradycardia, hypotension, and cardiac dysrhythmias. Therefore, diet history should carefully be obtained from the patients admitted with bradycardia and hypotension, and mad honey intoxication should also be considered in the

  9. Genome-wide identification, characterisation and expression analysis of the MADS-box gene family in Prunus mume.

    Xu, Zongda; Zhang, Qixiang; Sun, Lidan; Du, Dongliang; Cheng, Tangren; Pan, Huitang; Yang, Weiru; Wang, Jia

    2014-10-01

    MADS-box genes encode transcription factors that play crucial roles in plant development, especially in flower and fruit development. To gain insight into this gene family in Prunus mume, an important ornamental and fruit plant in East Asia, and to elucidate their roles in flower organ determination and fruit development, we performed a genome-wide identification, characterisation and expression analysis of MADS-box genes in this Rosaceae tree. In this study, 80 MADS-box genes were identified in P. mume and categorised into MIKC, Mα, Mβ, Mγ and Mδ groups based on gene structures and phylogenetic relationships. The MIKC group could be further classified into 12 subfamilies. The FLC subfamily was absent in P. mume and the six tandemly arranged DAM genes might experience a species-specific evolution process in P. mume. The MADS-box gene family might experience an evolution process from MIKC genes to Mδ genes to Mα, Mβ and Mγ genes. The expression analysis suggests that P. mume MADS-box genes have diverse functions in P. mume development and the functions of duplicated genes diverged after the duplication events. In addition to its involvement in the development of female gametophytes, type I genes also play roles in male gametophytes development. In conclusion, this study adds to our understanding of the roles that the MADS-box genes played in flower and fruit development and lays a foundation for selecting candidate genes for functional studies in P. mume and other species. Furthermore, this study also provides a basis to study the evolution of the MADS-box family.

  10. A Modular GIS-Based Software Architecture for Model Parameter Estimation using the Method of Anchored Distributions (MAD)

    Ames, D. P.; Osorio-Murillo, C.; Over, M. W.; Rubin, Y.

    2012-12-01

    The Method of Anchored Distributions (MAD) is an inverse modeling technique that is well-suited for estimation of spatially varying parameter fields using limited observations and Bayesian methods. This presentation will discuss the design, development, and testing of a free software implementation of the MAD technique using the open source DotSpatial geographic information system (GIS) framework, R statistical software, and the MODFLOW groundwater model. This new tool, dubbed MAD-GIS, is built using a modular architecture that supports the integration of external analytical tools and models for key computational processes including a forward model (e.g. MODFLOW, HYDRUS) and geostatistical analysis (e.g. R, GSLIB). The GIS-based graphical user interface provides a relatively simple way for new users of the technique to prepare the spatial domain, to identify observation and anchor points, to perform the MAD analysis using a selected forward model, and to view results. MAD-GIS uses the Managed Extensibility Framework (MEF) provided by the Microsoft .NET programming platform to support integration of different modeling and analytical tools at run-time through a custom "driver." Each driver establishes a connection with external programs through a programming interface, which provides the elements for communicating with core MAD software. This presentation gives an example of adapting the MODFLOW to serve as the external forward model in MAD-GIS for inferring the distribution functions of key MODFLOW parameters. Additional drivers for other models are being developed and it is expected that the open source nature of the project will engender the development of additional model drivers by 3rd party scientists.

  11. LA INFORMACIÓN GEOGRÁFICA EN INTRARREDES CORPORATIVAS. APLICACIÓN DE UN SIG PARA LA GESTIÓN DEL CAMPUS DE LA UNIVERSIDAD DE ALICANTE: SIG-UA

    Alfredo Ramón Morte

    1998-01-01

    Full Text Available SIG-UA es un proyecto de investigación de Geografía aplicada destinado a la elabo- ración y puesta en explotación de un sistema de información geográfica (SIG para la gestión de espacios e infraestructuras de la Universidad de Alicante. El objetivo es demostrar cómo la contemplación gráfica de la información contenida en bases de datos, es decir, su representación cartográfica, es el medio más eficaz para comprender y utilizar estrategias de actuación territorial, ventajas que se ven potenciadas al ser u sadas junto a otra forma muy sugerente de consulta de datos, los protocolos de transferencia de hipertextos en una red local de tipo corporativo. En apretada síntesis, los servidores WEB de aplicaciones SIG pueden estar llamados a convertirse en una extraordinaria opción para el control adecuado de los activos inmuebles, tanto en empresas privadas como en organismos públicos.

  12. Hvorfor støtter det eleverne at bevæge sig fysisk på en tallinje?

    Ejersbo, Lisser Rye

    2014-01-01

    Som reaktion på min forrige blok, vil jeg her uddybe og begrunde, hvorfor det er en god ide, at lade eleverne få kropslige oplevelser med tallinjen. Det drejer sig bl.a. om fænomenet SNARC, der er en forkortelse for ‘Spatical Numerical Association of Response Codes’, og som på godt dansk betyder,......, at venstre hånd er hurtigere til at reagere på små tal i forhold til at placere dem på en tallinje, mens højre hånd er hurtigere, når det drejer sig om større tal...

  13. Long Duration Exposure Facility (LDEF) optical systems SIG summary and database

    Bohnhoff-Hlavacek, Gail

    1992-01-01

    The main objectives of the Long Duration Exposure Facility (LDEF) Optical Systems Special Investigative Group (SIG) Discipline are to develop a database of experimental findings on LDEF optical systems and elements hardware, and provide an optical system overview. Unlike the electrical and mechanical disciplines, the optics effort relies primarily on the testing of hardware at the various principal investigator's laboratories, since minimal testing of optical hardware was done at Boeing. This is because all space-exposed optics hardware are part of other individual experiments. At this time, all optical systems and elements testing by experiment investigator teams is not complete, and in some cases has hardly begun. Most experiment results to date, document observations and measurements that 'show what happened'. Still to come from many principal investigators is a critical analysis to explain 'why it happened' and future design implications. The original optical system related concerns and the lessons learned at a preliminary stage in the Optical Systems Investigations are summarized. The design of the Optical Experiments Database and how to acquire and use the database to review the LDEF results are described.

  14. Long Duration Exposure Facility (LDEF) optical systems SIG summary and database

    Bohnhoff-Hlavacek, Gail

    1992-09-01

    The main objectives of the Long Duration Exposure Facility (LDEF) Optical Systems Special Investigative Group (SIG) Discipline are to develop a database of experimental findings on LDEF optical systems and elements hardware, and provide an optical system overview. Unlike the electrical and mechanical disciplines, the optics effort relies primarily on the testing of hardware at the various principal investigator's laboratories, since minimal testing of optical hardware was done at Boeing. This is because all space-exposed optics hardware are part of other individual experiments. At this time, all optical systems and elements testing by experiment investigator teams is not complete, and in some cases has hardly begun. Most experiment results to date, document observations and measurements that 'show what happened'. Still to come from many principal investigators is a critical analysis to explain 'why it happened' and future design implications. The original optical system related concerns and the lessons learned at a preliminary stage in the Optical Systems Investigations are summarized. The design of the Optical Experiments Database and how to acquire and use the database to review the LDEF results are described.

  15. SigWin-detector: a Grid-enabled workflow for discovering enriched windows of genomic features related to DNA sequences.

    Inda, Márcia A; van Batenburg, Marinus F; Roos, Marco; Belloum, Adam S Z; Vasunin, Dmitry; Wibisono, Adianto; van Kampen, Antoine H C; Breit, Timo M

    2008-08-08

    Chromosome location is often used as a scaffold to organize genomic information in both the living cell and molecular biological research. Thus, ever-increasing amounts of data about genomic features are stored in public databases and can be readily visualized by genome browsers. To perform in silico experimentation conveniently with this genomics data, biologists need tools to process and compare datasets routinely and explore the obtained results interactively. The complexity of such experimentation requires these tools to be based on an e-Science approach, hence generic, modular, and reusable. A virtual laboratory environment with workflows, workflow management systems, and Grid computation are therefore essential. Here we apply an e-Science approach to develop SigWin-detector, a workflow-based tool that can detect significantly enriched windows of (genomic) features in a (DNA) sequence in a fast and reproducible way. For proof-of-principle, we utilize a biological use case to detect regions of increased and decreased gene expression (RIDGEs and anti-RIDGEs) in human transcriptome maps. We improved the original method for RIDGE detection by replacing the costly step of estimation by random sampling with a faster analytical formula for computing the distribution of the null hypothesis being tested and by developing a new algorithm for computing moving medians. SigWin-detector was developed using the WS-VLAM workflow management system and consists of several reusable modules that are linked together in a basic workflow. The configuration of this basic workflow can be adapted to satisfy the requirements of the specific in silico experiment. As we show with the results from analyses in the biological use case on RIDGEs, SigWin-detector is an efficient and reusable Grid-based tool for discovering windows enriched for features of a particular type in any sequence of values. Thus, SigWin-detector provides the proof-of-principle for the modular e-Science based concept

  16. Des SIG-P pour sauver le complexe forestier de la basse rivière Tana?

    L'objectif général de ce programme panafricain est de contribuer à rendre disponibles des systèmes d'information de bonne qualité, fiables et accessibles grâce à l'usage de SIG-P en vue d'améliorer la gestion des ressources naturelles (eau, terres, forêts, etc.) et de promouvoir la sécurité alimentaire. Le programme ...

  17. Lingkungan Keluarga Anak-Anak Berprestasi Tinggi dan Anak-Anak Berprestasi Rendah di SMA Negeri Kabupaten Pidie Sigli

    Husaini Daud

    2016-02-01

    Full Text Available This study describes the contribution of family environment to the achievement of students. Data were collected from the high achievers and low achievers of SMAN grade II student at Kabupaten Pidie Sigli. The result reveals that 5 sub-variables of family environment do not show significant contribution to the student's achievement, while 6 of them give significant contributions though they are not applicable, and 8 of them are significant and applicable.

  18. SIG: Multiple Views on Safety-Critical Automation: Aircraft, Autonomous Vehicles, Air Traffic Management and Satellite Ground Segments Perspectives

    Feary, Michael; Palanque, Philippe; Martinie, Célia; Tscheligi, Manfred

    2016-01-01

    This SIG focuses on the engineering of automation in interactive critical systems. Automation has already been studied in a number of (sub-) disciplines and application fields: design, human factors, psychology, (software) engineering, aviation, health care, games. One distinguishing feature of the area we are focusing on is that in the field of interactive critical systems properties such as reliability, dependability, fault tolerance are as important as usability, user experience or overall acceptance issues. The SIG targets at two problem areas: first the engineering of the user interaction with (partly-) autonomous systems: how to design, build and assess autonomous behavior, especially in cases where there is a need to represent on the user interface both autonomous and interactive objects. An example of such integration is the representation of an unmanned aerial vehicle (UAV) (where no direct interaction is possible), together with aircrafts (that have to be instructed by an air traffic controller to avoid the UAV). Second the design and engineering of user interaction in general for autonomous objects/systems (for example a cruise control in a car or an autopilot in an aircraft). The goal of the SIG is to raise interest in the CHI community on the general aspects of automation and to identify a community of researchers and practitioners interested in those increasingly prominent issues of interfaces towards (semi)-autonomous systems. The expected audience should be interested in addressing the issues of integration of mainly unconnected research domains to formulate a new joint research agenda.

  19. Effects of the Strategic Prevention Framework State Incentives Grant (SPF SIG) on state prevention infrastructure in 26 states.

    Orwin, Robert G; Stein-Seroussi, Alan; Edwards, Jessica M; Landy, Ann L; Flewelling, Robert L

    2014-06-01

    The Strategic Prevention Framework State Incentive Grant (SPF SIG) program is a national public health initiative sponsored by the U.S. Substance Abuse and Mental Health Services Administration's Center for Substance Abuse Prevention to prevent substance abuse and its consequences. State grantees used a data-driven planning model to allocate resources to 450 communities, which in turn launched over 2,200 intervention strategies to target prevention priorities in their respective populations. An additional goal was to build prevention capacity and infrastructure at the state and community levels. This paper addresses whether the state infrastructure goal was achieved, and what contextual and implementation factors were associated with success. The findings are consistent with claims that, overall, the SPF SIG program met its goal of increasing prevention capacity and infrastructure across multiple infrastructure domains, though the mediating effects of implementation were evident only in the evaluation/monitoring domain. The results also show that an initiative like the SPF SIG, which could easily have been compartmentalized within the states, has the potential to permeate more broadly throughout state prevention systems.

  20. Interference Measurements in the European 868 MHz ISM Band with Focus on LoRa and SigFox

    Lauridsen, Mads; Vejlgaard, Benny; Kovács, István

    2017-01-01

    In this measurement study the signal activity and power levels are measured in the European Industrial, Scientific, and Medical band 863-870 MHz in the city of Aalborg, Denmark. The target is to determine if there is any interference, which may impact deployment of Internet of Things devices....... The focus is on the Low Power Wide Area technologies LoRa and SigFox. The measurements show that there is a 22-33 % probability of interfering signals above -105 dBm within the mandatory LoRa and SigFox 868.0-868.6 MHz band in a shopping area and a business park in downtown Aalborg, which thus limits...... the potential coverage and capacity of LoRa and SigFox. However, the probability of interference is less than 3 % in the three other measurement locations in Aalborg. Finally, a hospital and an industrial area are shown to experience high activity in the RFID subband 865-868 MHz, while the wireless audio band...

  1. Vaccinomics Approach for Designing Potential Peptide Vaccine by Targeting Shigella spp. Serine Protease Autotransporter Subfamily Protein SigA

    Arafat Rahman Oany

    2017-01-01

    Full Text Available Shigellosis, a bacillary dysentery, is closely associated with diarrhoea in human and causes infection of 165 million people worldwide per year. Casein-degrading serine protease autotransporter of enterobacteriaceae (SPATE subfamily protein SigA, an outer membrane protein, exerts both cytopathic and enterotoxic effects especially cytopathic to human epithelial cell type-2 (HEp-2 and is shown to be highly immunogenic. In the present study, we have tried to impose the vaccinomics approach for designing a common peptide vaccine candidate against the immunogenic SigA of Shigella spp. At first, 44 SigA proteins from different variants of S. flexneri, S. dysenteriae, S. boydii, and S. sonnei were assessed to find the most antigenic protein. We retrieved 12 peptides based on the highest score for human leukocyte antigen (HLA supertypes analysed by NetCTL. Initially, these peptides were assessed for the affinity with MHC class I and class II alleles, and four potential core epitopes VTARAGLGY, FHTVTVNTL, HTTWTLTGY, and IELAGTLTL were selected. From these, FHTVTVNTL and IELAGTLTL peptides were shown to have 100% conservancy. Finally, IELAGTLTL was shown to have the highest population coverage (83.86% among the whole world population. In vivo study of the proposed epitope might contribute to the development of functional and unique widespread vaccine, which might be an operative alleyway to thwart dysentery from the world.

  2. Characterization of an AGAMOUS-like MADS Box Protein, a Probable Constituent of Flowering and Fruit Ripening Regulatory System in Banana

    Roy Choudhury, Swarup; Roy, Sujit; Nag, Anish; Singh, Sanjay Kumar; Sengupta, Dibyendu N.

    2012-01-01

    The MADS-box family of genes has been shown to play a significant role in the development of reproductive organs, including dry and fleshy fruits. In this study, the molecular properties of an AGAMOUS like MADS box transcription factor in banana cultivar Giant governor (Musa sp, AAA group, subgroup Cavendish) has been elucidated. We have detected a CArG-box sequence binding AGAMOUS MADS-box protein in banana flower and fruit nuclear extracts in DNA-protein interaction assays. The protein fraction in the DNA-protein complex was analyzed by mass spectrometry and using this information we have obtained the full length cDNA of the corresponding protein. The deduced protein sequence showed ∼95% amino acid sequence homology with MA-MADS5, a MADS-box protein described previously from banana. We have characterized the domains of the identified AGAMOUS MADS-box protein involved in DNA binding and homodimer formation in vitro using full-length and truncated versions of affinity purified recombinant proteins. Furthermore, in order to gain insight about how DNA bending is achieved by this MADS-box factor, we performed circular permutation and phasing analysis using the wild type recombinant protein. The AGAMOUS MADS-box protein identified in this study has been found to predominantly accumulate in the climacteric fruit pulp and also in female flower ovary. In vivo and in vitro assays have revealed specific binding of the identified AGAMOUS MADS-box protein to CArG-box sequence in the promoters of major ripening genes in banana fruit. Overall, the expression patterns of this MADS-box protein in banana female flower ovary and during various phases of fruit ripening along with the interaction of the protein to the CArG-box sequence in the promoters of major ripening genes lead to interesting assumption about the possible involvement of this AGAMOUS MADS-box factor in banana fruit ripening and floral reproductive organ development. PMID:22984496

  3. Overexpression of a novel MADS-box gene SlFYFL delays senescence, fruit ripening and abscission in tomato

    Xie, Qiaoli; Hu, Zongli; Zhu, Zhiguo; Dong, Tingting; Zhao, Zhiping; Cui, Baolu; Chen, Guoping

    2014-03-01

    MADS-domain proteins are important transcription factors involved in many biological processes of plants. In our study, a tomato MADS-box gene, SlFYFL, was isolated. SlFYFL is expressed in all tissues of tomato and significantly higher in mature leave, fruit of different stages, AZ (abscission zone) and sepal. Delayed leaf senescence and fruit ripening, increased storability and longer sepals were observed in 35S:FYFL tomato. The accumulation of carotenoid was reduced, and ethylene content, ethylene biosynthetic and responsive genes were down-regulated in 35S:FYFL fruits. Abscission zone (AZ) did not form normally and abscission zone development related genes were declined in AZs of 35S:FYFL plants. Yeast two-hybrid assay revealed that SlFYFL protein could interact with SlMADS-RIN, SlMADS1 and SlJOINTLESS, respectively. These results suggest that overexpression of SlFYFL regulate fruit ripening and development of AZ via interactions with the ripening and abscission zone-related MADS box proteins.

  4. Identification of a Sgo2-Dependent but Mad2-Independent Pathway Controlling Anaphase Onset in Fission Yeast

    John C. Meadows

    2017-02-01

    Full Text Available The onset of anaphase is triggered by activation of the anaphase-promoting complex/cyclosome (APC/C following silencing of the spindle assembly checkpoint (SAC. APC/C triggers ubiquitination of Securin and Cyclin B, which leads to loss of sister chromatid cohesion and inactivation of Cyclin B/Cdk1, respectively. This promotes relocalization of Aurora B kinase and other components of the chromosome passenger complex (CPC from centromeres to the spindle midzone. In fission yeast, this is mediated by Clp1 phosphatase-dependent interaction of CPC with Klp9/MKLP2 (kinesin-6. When this interaction is disrupted, kinetochores bi-orient normally, but APC/C activation is delayed via a mechanism that requires Sgo2 and some (Bub1, Mph1/Mps1, and Mad3, but not all (Mad1 and Mad2, components of the SAC and the first, but not second, lysine, glutamic acid, glutamine (KEN box in Mad3. These data indicate that interaction of CPC with Klp9 terminates a Sgo2-dependent, but Mad2-independent, APC/C-inhibitory pathway that is distinct from the canonical SAC.

  5. Regulatory role of OsMADS34 in the determination of glumes fate, grain yield and quality in rice

    Deyong Ren

    2016-12-01

    Full Text Available Grasses produce seeds on spikelets, a unique type of inflorescence. Despite the importance of grass crops for food, the genetic mechanisms that control spikelet development remain poorly understood. In this study, we used m34-z, a new mutant allele of the rice (Oryza sativa E-class gene OsMADS34, to examine OsMADS34 function in determining the identities of glumes (rudimentary glume and sterile lemma and grain size. In the m34-z mutant, both the rudimentary glume and sterile lemma were homeotically converted to the lemma-like organs and acquired the lemma identity, suggesting that OsMADS34 plays important roles in the development of glumes. In the m34-z mutant, most of the grains from the secondary panicle branches were decreased in size, compared with grains from wild type, but no differences were observed in the grains from the primary panicle branches. The amylose content and gel consistency, and a seed-setting rate from the secondary panicle branches were reduced in the m34-z mutant. Interesting, transcriptional activity analysis revealed that OsMADS34 protein was a transcription repressor and it may influence grain yield by suppressing the expressions of BG1, GW8, GW2 and GL7 in the m34-z mutant. These findings revealed that OsMADS34 largely affects grain yield by affecting the size of grains from the secondary branches.

  6. Novel Mad2-targeting miR-493-3p controls mitotic fidelity and cancer cells' sensitivity to paclitaxel.

    Tambe, Mahesh; Pruikkonen, Sofia; Mäki-Jouppila, Jenni; Chen, Ping; Elgaaen, Bente Vilming; Straume, Anne Hege; Huhtinen, Kaisa; Cárpen, Olli; Lønning, Per Eystein; Davidson, Ben; Hautaniemi, Sampsa; Kallio, Marko J

    2016-03-15

    The molecular pathways that contribute to the proliferation and drug response of cancer cells are highly complex and currently insufficiently characterized. We have identified a previously unknown microRNA-based mechanism that provides cancer cells means to stimulate tumorigenesis via increased genomic instability and, at the same time, evade the action of clinically utilized microtubule drugs. We demonstrate miR-493-3p to be a novel negative regulator of mitotic arrest deficient-2 (MAD2), an essential component of the spindle assembly checkpoint that monitors the fidelity of chromosome segregation. The microRNA targets the 3' UTR of Mad2 mRNA thereby preventing translation of the Mad2 protein. In cancer cells, overexpression of miR-493-3p induced a premature mitotic exit that led to increased frequency of aneuploidy and cellular senescence in the progeny cells. Importantly, excess of the miR-493-3p conferred resistance of cancer cells to microtubule drugs. In human neoplasms, miR-493-3p and Mad2 expression alterations correlated with advanced ovarian cancer forms and high miR-493-3p levels were associated with reduced survival of ovarian and breast cancer patients with aggressive tumors, especially in the paclitaxel therapy arm. Our results suggest that intratumoral profiling of miR-493-3p and Mad2 levels can have diagnostic value in predicting the efficacy of taxane chemotherapy.

  7. ¿Qué Teoría? La Teoría en Mad Money | What Theory? The Theory in Mad Money

    Susan STRANGE

    2012-10-01

    Full Text Available Mad Money (Manchester University Press, 1998 es la versión completamente reescrita y actualizada de Casino Capitalism (Blackwells, 1986. Se ha sugerido —de ambos volúmenes— que no había en ellos una teoría subyacente en la discusión de Strange sobre el sistema financiero internacional. Esto, argumenta Strange en este working paper, no es en absoluto el caso. Los dos volúmenes se sustentan, siempre implícitamente y a veces explícitamente, en los temas dominantes del trabajo de Strange desde la publicación de "International Relations and International Economics: A Case of Mutual Neglect", International Affairs, 46, (2, 1970. Se trata de tres temas: primero, una necesidad de privilegiar las políticas del sistema financiero internacional en el estudio de las relaciones internacionales; una disciplina miope desde hace mucho, concentrada en el conflicto violento y en la guerra entre estados, a expensas de todo el resto. Segundo, una necesidad de ir más alla de la teoría política y económica liberal y de reconocer el significado del "poder estructural" en el sistema internacional. Tercero, una necesidad de reconocer que las "áreas de ignorancia significativa" dentro de nuestra comprensión del rol del sistema financiero internacional en una era de revolución tecnológica y globalización son cada vez mayores. Para Strange, el poder estructural del capital no es constante y, por ende, no puede acomodarse en la lógica de la economía liberal. Así, usando la definición de "loco" del diccionario —comportamiento errático, impredecible e irracional que daña no solo a quien lo sufre sino también a otros—, tenemos, como ella dice, un "dinero loco"."Mad Money" (Manchester University Press, 1998 is the completely rewritten and updated version of "Casino Capitalism" (Blackwells, 1986. It has been suggested —of both volumes— that there was no theory underlying Strange's discussion of the international financial system in them

  8. Escherichia coli MltA : MAD phasing and refinement of a tetartohedrally twinned protein crystal structure (vol D61, pg 613, 2005)

    Barends, Thomas R.M.; Jong, René M. de; Straaten, Karin E. van; Thunnissen, Andy-Mark W.H.; Dijkstra, Bauke W.

    Crystals were grown of a mutant form of the bacterial cell-wall maintenance protein MltA that diffracted to 2.15 Å resolution. When phasing with molecular replacement using the native structure failed, selenium MAD was used to obtain initial phases. However, after MAD phasing the crystals were found

  9. Divergence of recently duplicated M{gamma}-type MADS-box genes in Petunia.

    Bemer, Marian; Gordon, Jonathan; Weterings, Koen; Angenent, Gerco C

    2010-02-01

    The MADS-box transcription factor family has expanded considerably in plants via gene and genome duplications and can be subdivided into type I and MIKC-type genes. The two gene classes show a different evolutionary history. Whereas the MIKC-type genes originated during ancient genome duplications, as well as during more recent events, the type I loci appear to experience high turnover with many recent duplications. This different mode of origin also suggests a different fate for the type I duplicates, which are thought to have a higher chance to become silenced or lost from the genome. To get more insight into the evolution of the type I MADS-box genes, we isolated nine type I genes from Petunia, which belong to the Mgamma subclass, and investigated the divergence of their coding and regulatory regions. The isolated genes could be subdivided into two categories: two genes were highly similar to Arabidopsis Mgamma-type genes, whereas the other seven genes showed less similarity to Arabidopsis genes and originated more recently. Two of the recently duplicated genes were found to contain deleterious mutations in their coding regions, and expression analysis revealed that a third paralog was silenced by mutations in its regulatory region. However, in addition to the three genes that were subjected to nonfunctionalization, we also found evidence for neofunctionalization of one of the Petunia Mgamma-type genes. Our study shows a rapid divergence of recently duplicated Mgamma-type MADS-box genes and suggests that redundancy among type I paralogs may be less common than expected.

  10. Sigand : une application SIG sous androïd (tablette de la gestion des chantiers

    CHAKIB SAFAR BATI

    2016-12-01

    Full Text Available L’introduction de données terrain pour les études environnementales ou de gestion de l’espace, régionales ou locales est une nécessité incontournable pour la validation des traitements et des interprétations mais aussi pour fournir des compléments d’informations aux divers travaux. Mais ce retour terrain est également de plus en plus demandé pour les gestionnaires de chantiers et de projets qui ont une exigence de contrôle ou de suivi et donc d’une information temps réel sur l’avancement des travaux, autrement pour introduire de nouvelles informations sur la base de données géoréférencées. Les avancées technologiques en matière de manipulation de l’information géographique (GPS, tablette graphique, téléphone portable, réalité augmentée permettent d’espérer la mise à dispositiondes professionnels, voire d’un plus large public, des outils adéquats pour de tels travaux. Actuellement, les outils d’élaboration, de gestion et d’exploitation des bases de données géographiques (solutions SIG ne sont pas encore complètement opérationnels en version « tablette » ; des applications telles que Qgis ou ArcGis sous Androïd attendent encore des développements. Nous présentons ici la première version d’une application SIG sous Androïd (disponible sur tablette pour la gestion en temps réel des chantiers qui permet de charger des couches vectorielles et de les mettre à jour sur le terrain en fonction de l’observation directe ; les couches (préalablement géoréférencées sont projetées sur des fonds cartographiques fournis par un serveur cartographique Google Maps (Route, Satellite, terrain; dans le cas d’absence de connexion internet, le fond cartographique est ramené d’un cache. Nous utilisons SpatialiteAndroïd pour le stockage et la manipulation des données géographiques. Le contrôleur de cartes Google Maps est employé pour afficher en mode cartographique les données stockées dans

  11. From MAD to SAD: The Italian experience for the low-frequency aperture array of SKA1-LOW

    Bolli, P.; Pupillo, G.; Virone, G.; Farooqui, M. Z.; Lingua, A.; Mattana, A.; Monari, J.; Murgia, M.; Naldi, G.; Paonessa, F.; Perini, F.; Pluchino, S.; Rusticelli, S.; Schiaffino, M.; Schillirò, F.; Tartarini, G.; Tibaldi, A.

    2016-03-01

    This paper describes two small aperture array demonstrators called Medicina and Sardinia Array Demonstrators (MAD and SAD, respectively). The objectives of these instruments are to acquire experience and test new technologies for a possible application to the low-frequency aperture array of the low-frequency telescope of the Square Kilometer Array phase 1 (SKA1-LOW). The MAD experience was concluded in 2014, and it turned out to be an important test bench for implementing calibration techniques based on an artificial source mounted in an aerial vehicle. SAD is based on 128 dual-polarized Vivaldi antennas and is 1 order of magnitude larger than MAD. The architecture and the station size of SAD, which is along the construction phase, are more similar to those under evaluation for SKA1-LOW, and therefore, SAD is expected to provide useful hints for SKA1-LOW.

  12. Thermogravimetric pyrolysis kinetics of bamboo waste via Asymmetric Double Sigmoidal (Asym2sig) function deconvolution.

    Chen, Chuihan; Miao, Wei; Zhou, Cheng; Wu, Hongjuan

    2017-02-01

    Thermogravimetric kinetic of bamboo waste (BW) pyrolysis has been studied using Asymmetric Double Sigmoidal (Asym2sig) function deconvolution. Through deconvolution, BW pyrolytic profiles could be separated into three reactions well, each of which corresponded to pseudo hemicelluloses (P-HC), pseudo cellulose (P-CL), and pseudo lignin (P-LG) decomposition. Based on Friedman method, apparent activation energy of P-HC, P-CL, P-LG was found to be 175.6kJ/mol, 199.7kJ/mol, and 158.4kJ/mol, respectively. Energy compensation effects (lnk 0, z vs. E z ) of pseudo components were in well linearity, from which pre-exponential factors (k 0 ) were determined as 6.22E+11s -1 (P-HC), 4.50E+14s -1 (P-CL) and 1.3E+10s -1 (P-LG). Integral master-plots results showed pyrolytic mechanism of P-HC, P-CL, and P-LG was reaction order of f(α)=(1-α) 2 , f(α)=1-α and f(α)=(1-α) n (n=6-8), respectively. Mechanism of P-HC and P-CL could be further reconstructed to n-th order Avrami-Erofeyev model of f(α)=0.62(1-α)[-ln(1-α)] -0.61 (n=0.62) and f(α)=1.08(1-α)[-ln(1-α)] 0.074 (n=1.08). Two-steps reaction was more suitable for P-LG pyrolysis. Copyright © 2016 Elsevier Ltd. All rights reserved.

  13. [Foucault, Derrida, and the history of madness: notes on a controversy].

    Pereira Neto, A F

    1998-01-01

    The publication of the book Folie et Déraison. Histoire de la Folie à l'Age Classique (1961), by Michel Foucault, sparked a debate between the author and philosopher Jacques Derrida during the 1960s and 70s. Derrida criticized the methodological proposal and organization of the History of Madness presented by Foucault in the foreword to the first edition. The controversy appears to have motivated the author to withdraw this same foreword from the second edition. The purpose of this article is to analyze some current points in this controversy. It also presents a research agenda for an understanding of the reasons leading Foucault to take this stance.

  14. The Killer Will Remain Free: On Pat Parker and the Poetics of Madness.

    Ali, Kazim

    2015-01-01

    Poet and scholar Kazim Ali reads Pat Parker's Movement in Black intimately, one poet to another, uncovering the shadow-fact of the lives of most people of color: not only the anger that is somehow sublimated into every part of our lives but also the issue that carrying this feeling around has on our mental health itself-that "anger" and "madness" might have sources in one another. Ali concludes that Parker offers a brutal and clear-eyed and ultimately hopeful assessment of the conditions that were faced at the time, and even now, by communities of color.

  15. Automatic change detection in RapidEye data using the combined MAD and kernel MAF methods

    Nielsen, Allan Aasbjerg; Hecheltjen, Antje; Thonfeld, Frank

    2010-01-01

    The IR-MAD components show changes for a large part of the entire subset. Especially phenological changes in the agricultural fields surrounding the open pit are predominant. As opposed to this, kMAF components focus more on changes in the open-cast mine (and changes due to the two clouds...... and their shadows, not visible in the zoom). Ground data were available from bucket-wheel excavators on the extraction side (to the northwest in the open pit) in terms of elevation data for both dates. No ground data were available for changes due to backfill (southeastern part of the open pit) or changes due...

  16. Low MAD2 expression levels associate with reduced progression-free survival in patients with high-grade serous epithelial ovarian cancer.

    Furlong, Fiona

    2012-04-01

    Epithelial ovarian cancer (EOC) has an innate susceptibility to become chemoresistant. Up to 30% of patients do not respond to conventional chemotherapy [paclitaxel (Taxol®) in combination with carboplatin] and, of those who have an initial response, many patients relapse. Therefore, an understanding of the molecular mechanisms that regulate cellular chemotherapeutic responses in EOC cells has the potential to impact significantly on patient outcome. The mitotic arrest deficiency protein 2 (MAD2), is a centrally important mediator of the cellular response to paclitaxel. MAD2 immunohistochemical analysis was performed on 82 high-grade serous EOC samples. A multivariate Cox regression analysis of nuclear MAD2 IHC intensity adjusting for stage, tumour grade and optimum surgical debulking revealed that low MAD2 IHC staining intensity was significantly associated with reduced progression-free survival (PFS) (p = 0.0003), with a hazard ratio of 4.689. The in vitro analyses of five ovarian cancer cell lines demonstrated that cells with low MAD2 expression were less sensitive to paclitaxel. Furthermore, paclitaxel-induced activation of the spindle assembly checkpoint (SAC) and apoptotic cell death was abrogated in cells transfected with MAD2 siRNA. In silico analysis identified a miR-433 binding domain in the MAD2 3\\' UTR, which was verified in a series of experiments. Firstly, MAD2 protein expression levels were down-regulated in pre-miR-433 transfected A2780 cells. Secondly, pre-miR-433 suppressed the activity of a reporter construct containing the 3\\'-UTR of MAD2. Thirdly, blocking miR-433 binding to the MAD2 3\\' UTR protected MAD2 from miR-433 induced protein down-regulation. Importantly, reduced MAD2 protein expression in pre-miR-433-transfected A2780 cells rendered these cells less sensitive to paclitaxel. In conclusion, loss of MAD2 protein expression results in increased resistance to paclitaxel in EOC cells. Measuring MAD2 IHC staining intensity may predict

  17. Metformin Protects Neurons against Oxygen-Glucose Deprivation/Reoxygenation -Induced Injury by Down-Regulating MAD2B.

    Meng, Xianfang; Chu, Guangpin; Yang, Zhihua; Qiu, Ping; Hu, Yue; Chen, Xiaohe; Peng, Wenpeng; Ye, Chen; He, Fang-Fang; Zhang, Chun

    2016-01-01

    Metformin, the common medication for type II diabetes, has protective effects on cerebral ischemia. However, the molecular mechanisms are far from clear. Mitotic arrest deficient 2-like protein 2 (MAD2B), an inhibitor of the anaphase-promoting complex (APC), is widely expressed in hippocampal and cortical neurons and plays an important role in mediating high glucose-induced neurotoxicity. The present study investigated whether metformin modifies the expression of MAD2B and to exert its neuroprotective effects in primary cultured cortical neurons during oxygen-glucose deprivation/reoxygenation (OGD/R), a widely used in vitro model of ischemia/reperfusion. Primary cortical neurons were cultured, deprived of oxygen-glucose for 1 h, and then recovered with oxygen-glucose for 12 h and 24 h. Cell viability was measured by detecting the levels of lactate dehydrogenase (LDH) in culture medium. The levels of MAD2B, cyclin B and p-histone 3 were measured by Western blot. Cell viability of neurons was reduced under oxygen-glucose deprivation/reoxygenation (OGD/R). The expression of MAD2B was increased under OGD/R. The levels of cyclin B1, which is a substrate of APC, were also increased. Moreover, OGD/R up-regulated the phosphorylation levels of histone 3, which is the induction of aberrant re-entry of post-mitotic neurons. However, pretreatment of neurons with metformin alleviated OGD/R-induced injury. Metformin further decreased the expression of MAD2B, cyclin B1 and phosphorylation levels of histone 3. Metformin exerts its neuroprotective effect through regulating the expression of MAD2B in neurons under OGD/R. © 2016 The Author(s) Published by S. Karger AG, Basel.

  18. Pharmacology and psychiatry at the origins of Greek medicine: The myth of Melampus and the madness of the Proetides.

    Olivieri, Matteo F; Marzari, Francesca; Kesel, Andreas J; Bonalume, Laura; Saettini, Francesco

    2017-01-01

    Melampus is a seer-healer of Greek myth attributed with having healed the young princesses of Argos of madness. Analysis of this legend and its sources sheds light on the early stages of the "medicalizing" shift in the history of ancient Greek medicine. Retrospective psychological diagnosis suggests that the descriptions of the youths' madness rose from actual observation of behavioral and mental disorders. Melampus is credited with having healed them by administering hellebore. Pharmacological analysis of botanical specimens proves that Helleborus niger features actual neurological properties effective in the treatment of mental disorders. The discussion aims at examining the rational aspects of the treatment of mental conditions in Greco-Roman antiquity.

  19. Validation of Cut-Points for Evaluating the Intensity of Physical Activity with Accelerometry-Based Mean Amplitude Deviation (MAD.

    Henri Vähä-Ypyä

    Full Text Available Our recent study of three accelerometer brands in various ambulatory activities showed that the mean amplitude deviation (MAD of the resultant acceleration signal performed best in separating different intensity levels and provided excellent agreement between the three devices. The objective of this study was to derive a regression model that estimates oxygen consumption (VO2 from MAD values and validate the MAD-based cut-points for light, moderate and vigorous locomotion against VO2 within a wide range of speeds.29 participants performed a pace-conducted non-stop test on a 200 m long indoor track. The initial speed was 0.6 m/s and it was increased by 0.4 m/s every 2.5 minutes until volitional exhaustion. The participants could freely decide whether they preferred to walk or run. During the test they carried a hip-mounted tri-axial accelerometer and mobile metabolic analyzer. The MAD was calculated from the raw acceleration data and compared to directly measured incident VO2. Cut-point between light and moderate activity was set to 3.0 metabolic equivalent (MET, 1 MET = 3.5 ml · kg-1 · min-1 and between moderate and vigorous activity to 6.0 MET as per standard use.The MAD and VO2 showed a very strong association. Within individuals, the range of r values was from 0.927 to 0.991 providing the mean r = 0.969. The optimal MAD cut-point for 3.0 MET was 91 mg (milligravity and 414 mg for 6.0 MET.The present study showed that the MAD is a valid method in terms of the VO2 within a wide range of ambulatory activities from slow walking to fast running. Being a device-independent trait, the MAD facilitates directly comparable, accurate results on the intensity of physical activity with all accelerometers providing tri-axial raw data.

  20. The Rhetoric of Madness in Kathy Acker’s Don Quixote

    Claudia Cao

    2017-07-01

    Full Text Available This essay examines the rhetorical experimentation of Don Quixote by Kathy Acker, starting from a theoretical concept central in the author’s thought: her search for a “language of the body”. A brief introduction to Kathy Acker’s plagiaristic poetics frames her narrative strategies between postmodern rewriting and pastiche. It shows the way in which her Don Quixote transposes the representative scheme of the chivalric quest into the contemporary value system with the aim of questioning Cervantes’ text as one of the canonical works of the Western literary tradition. The following section deepens three focal concepts of Acker’s theoretical works – body, madness, and norm – illuminating the connections between her rhetorical experimentation and the works by Luce Irigaray and Judith Butler. Finally, the paper will demonstrate how these concepts in Acker's rewriting of Don Quixote are strictly related to paradox, which she uses in order to actualize a “language of the body”: in the passage from the former novel to the postmodern work, madness has become the device which, from the semantic level to the rhetorical one, expresses Acker’s idea of language of the body, as an alternative and in contrast to the canonical language of the logos.

  1. MadAnalysis Recast Code for Dark Matter Production in Association with Bottom Quarks

    Biswas, Diptaparna

    The CMS-B2G-15-007 analysis was carried out to investigate the $pp\\rightarrow bb+DM$ process, which is a candidate process of direct Dark Matter production. In this particular work, this process is simulated and analyzed for 13 TeV LHC experiment using MadGraph, Pythia 8 and MadAnalysis C++ API. The recast code is written to reproduce the results obtained by the experimentalists in CMS-B2G-15-007 analysis. The result is interpreted within simplified scalar model in terms of the coupling between the mediator and the DM candidate and evaluated based on the MET distribution. CMS-B2G-15-007 uses a dataset of $2.17fb^{-1}$ of data collected by the CMS experiment in $\\sqrt{s}=13TeV$ proton-proton collisions at LHC. This analysis is sensitive also to DM production processes in association with top quarks. Results are reported as upper limits on the cross section for the b quark and top quark associated production independently, and interpreted within simplified models in terms of the coupling between the mediator an...

  2. TRIP13 is a protein-remodeling AAA+ ATPase that catalyzes MAD2 conformation switching.

    Ye, Qiaozhen; Rosenberg, Scott C; Moeller, Arne; Speir, Jeffrey A; Su, Tiffany Y; Corbett, Kevin D

    2015-04-28

    The AAA+ family ATPase TRIP13 is a key regulator of meiotic recombination and the spindle assembly checkpoint, acting on signaling proteins of the conserved HORMA domain family. Here we present the structure of the Caenorhabditis elegans TRIP13 ortholog PCH-2, revealing a new family of AAA+ ATPase protein remodelers. PCH-2 possesses a substrate-recognition domain related to those of the protein remodelers NSF and p97, while its overall hexameric architecture and likely structural mechanism bear close similarities to the bacterial protein unfoldase ClpX. We find that TRIP13, aided by the adapter protein p31(comet), converts the HORMA-family spindle checkpoint protein MAD2 from a signaling-active 'closed' conformer to an inactive 'open' conformer. We propose that TRIP13 and p31(comet) collaborate to inactivate the spindle assembly checkpoint through MAD2 conformational conversion and disassembly of mitotic checkpoint complexes. A parallel HORMA protein disassembly activity likely underlies TRIP13's critical regulatory functions in meiotic chromosome structure and recombination.

  3. TRIP13 is a protein-remodeling AAA+ ATPase that catalyzes MAD2 conformation switching

    Ye, Qiaozhen [Ludwig Institute for Cancer Research, San Diego Branch, La Jolla, United States; Rosenberg, Scott C. [Ludwig Institute for Cancer Research, San Diego Branch, La Jolla, United States; Moeller, Arne [National Resource for Automated Molecular Microscopy, Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, United States; Speir, Jeffrey A. [National Resource for Automated Molecular Microscopy, Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, United States; Su, Tiffany Y. [Ludwig Institute for Cancer Research, San Diego Branch, La Jolla, United States; Corbett, Kevin D. [Ludwig Institute for Cancer Research, San Diego Branch, La Jolla, United States; Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, United States

    2015-04-28

    The AAA+ family ATPase TRIP13 is a key regulator of meiotic recombination and the spindle assembly checkpoint, acting on signaling proteins of the conserved HORMA domain family. Here we present the structure of the Caenorhabditis elegans TRIP13 ortholog PCH-2, revealing a new family of AAA+ ATPase protein remodelers. PCH-2 possesses a substrate-recognition domain related to those of the protein remodelers NSF and p97, while its overall hexameric architecture and likely structural mechanism bear close similarities to the bacterial protein unfoldase ClpX. We find that TRIP13, aided by the adapter protein p31(comet), converts the HORMA-family spindle checkpoint protein MAD2 from a signaling-active ‘closed’ conformer to an inactive ‘open’ conformer. We propose that TRIP13 and p31(comet) collaborate to inactivate the spindle assembly checkpoint through MAD2 conformational conversion and disassembly of mitotic checkpoint complexes. A parallel HORMA protein disassembly activity likely underlies TRIP13's critical regulatory functions in meiotic chromosome structure and recombination.

  4. Proceedings of the fourth High-Energy Physics International Conference HEP-MAD 09

    Narison, S.

    2009-01-01

    This is the 4th of the series of HEP-MAD conference organized regularly every 2 or 3 years in Madagascar, in alternance to the traditional series of QCD-conference held in Montpellier (France) on July. The conference is expected to involve few numbers of physicists from abroad and equal numbers of experimental and theoretical high-energy physicists.Unlike the QCD conference which is a specialized meeting, HEP-MAD aims to have a wide view of physics: from High-Energy to Astro Physics. National contributions cover Nuclear and Environment Physics and the new form of energies (solar,...). This conference is a compromise between a standard one where specialized topics are presented and an introductory school to each subjects.It gives the opportunuity for high-energy physicists to promote the field of high-energy physics (theory and experiments)in Madagascar. In the same time, the meeting will permit to the participants to discover the country (well-known about its bio-diversity and rare animal and plant species) and its tradition and population from different origins.The theoretical and experimental talks cover different aspects of high-energy physics which are in the form of introductional reviews to the field, short contributions and posters. These talks are complemented by other national talks in other areas of physics. The Conference is expected to be published on-line by SLAC in a eConf-proceedings.

  5. Integrasi Model SWAT dan SIG dalam Upaya Menekan Laju Erosi DAD Deli, Sumatera Utara

    Riki Rahmad

    2017-04-01

    Full Text Available Penelitian ini bertujuan untuk melakukan simulasi model SWAT dalam menghitung besarnya laju erosi DAS, menguji kesesuaian model dalam memprediksi erosi, serta menentukan skenario penggunaan lahan yang paling optimal menurunkan laju erosi. Penelitian ini dilakukan di DAS Deli, Sumatera Utara. Pada penelitian ini analisis SWAT dilakukan dengan bantuan Sistem Informasi Geografi (SIG melalui 4 proses yaitu delineasi, pembentukan Hydrological Response Unit (HRU, pengolahan data dan simulasi, serta proses visualisasi. Hasil penelitian menunjukkan besarnya erosi rata-rata DAS Deli adalah 410,72 ton/ha/tahun. Hasil ini menunjukkan DAS Deli termasuk kedalam Tingkat Bahaya Erosi (TBE kategori Berat dengan kriteria sangat berat 37,04%, berat 17,07%, sedang 21,46%, ringan 17,38%, dan sangat ringan 7,04%. Uji validasi model menunjukkan bahwa ada hubungan positif antara debit model dan observasi dimana persentase perbedaan nilainya sangat kecil yang artinya besarnya debit model hampir sangat mendekati besarnya debit observasi, serta model dikategorikan sangat baik dalam melakukan simulasi debit aliran harian pada Sungai Deli. Metode skenario adalah berdasarkan analisis TBE. Hasil skenario penggunaan lahan berhasil menurunkan laju erosi DAS Deli 34,78% menjadi 267,88 ton/ha/tahun. This study aims to conduct SWAT model simulation calculation of the rate of erosion of the watershed, testing the suitability of the model in predicting erosion, land use scenarios and determine the most optimal decrease the rate of erosion. This research was conducted in the watershed Deli, North Sumatra. In this study, SWAT analysis performed with the help of Geographical Information Systems (GIS through 4 delineation process, namely, the establishment of Hydrological Response Unit (HRU, data processing and simulation, as well as the visualization process. The results show the average amount of erosion DAS Deli is 410.72 ton/ha/year. These results indicate DAS Deli included into

  6. SigWin-detector: a Grid-enabled workflow for discovering enriched windows of genomic features related to DNA sequences

    Wibisono Adianto

    2008-08-01

    Full Text Available Abstract Background Chromosome location is often used as a scaffold to organize genomic information in both the living cell and molecular biological research. Thus, ever-increasing amounts of data about genomic features are stored in public databases and can be readily visualized by genome browsers. To perform in silico experimentation conveniently with this genomics data, biologists need tools to process and compare datasets routinely and explore the obtained results interactively. The complexity of such experimentation requires these tools to be based on an e-Science approach, hence generic, modular, and reusable. A virtual laboratory environment with workflows, workflow management systems, and Grid computation are therefore essential. Findings Here we apply an e-Science approach to develop SigWin-detector, a workflow-based tool that can detect significantly enriched windows of (genomic features in a (DNA sequence in a fast and reproducible way. For proof-of-principle, we utilize a biological use case to detect regions of increased and decreased gene expression (RIDGEs and anti-RIDGEs in human transcriptome maps. We improved the original method for RIDGE detection by replacing the costly step of estimation by random sampling with a faster analytical formula for computing the distribution of the null hypothesis being tested and by developing a new algorithm for computing moving medians. SigWin-detector was developed using the WS-VLAM workflow management system and consists of several reusable modules that are linked together in a basic workflow. The configuration of this basic workflow can be adapted to satisfy the requirements of the specific in silico experiment. Conclusion As we show with the results from analyses in the biological use case on RIDGEs, SigWin-detector is an efficient and reusable Grid-based tool for discovering windows enriched for features of a particular type in any sequence of values. Thus, SigWin-detector provides the

  7. Los sistemas de información geográfica (sig), una gran herramienta para la silvicultura urbana.

    Otaya Burbano, Leodán Andrés; Sánhez Zapata, Robinson de Jesús; Morales Soto, León; Botero Fernández, Verónica

    2011-01-01

    Como una fase piloto, para adquirir experiencia, definir metodologías y determinar las ventajas de los Sistemas de Información Geográfica (SIG) al ser aplicados en la Silvicultura Urbana, se realizó el inventario, diagnóstico, propuesta de manejo y valoración económica del Bosque Urbano del barrio La Magnolia del Municipio de Envigado, Departamento de Antioquia, Colombia. Para el manejo y análisis de los registros recolectados en campo, se diseñó una base de datos en el software Microsoft Acc...

  8. Modelo de sistemas de informação técnica baseado numa plataforma SIG

    Almeida, António Manuel Galinho Pires de

    2006-01-01

    Dissertação apresentada como requisito parcial para obtenção do grau de Mestre em Ciência e Sistemas de Informação Geográfica O presente trabalho pretende desenvolver um modelo conceptual de um Sistema de Informação Técnica (SIT) baseado numa plataforma SIG, aplicado à Industria, mais especificamente à rede eléctrica de uma fábrica, apresentando ao mesmo tempo a metodologia a seguir na integração do modelo numa organização, e as vantagens que uma ferramenta como esta poderá pro...

  9. The extracytoplasmic function sigma factor SigY is important for efficient maintenance of the Spβ prophage that encodes sublancin in Bacillus subtilis.

    Mendez, Rebecca; Gutierrez, Alba; Reyes, Jasmin; Márquez-Magaña, Leticia

    2012-06-01

    Many strains of the soil bacterium Bacillus subtilis are capable of producing and being resistant to the antibiotic sublancin because they harbor the Spβ prophage. This 135 kb viral genome is integrated into the circular DNA chromosome of B. subtilis, and contains genes for the production of and resistance to sublancin. We investigated the role of SigY in sublancin production and resistance, finding that it is important for efficient maintenance of the Spβ prophage. We were unable to detect the prophage in mutants lacking SigY. Additionally, these mutants were no longer able to produce sublancin, were sensitive to killing by this factor, and displayed a delay in sporulation. Wild-type cells with normal SigY activity were found to partially lose the Spβ prophage during growth and early sporulation, suggesting a mechanism for the bistable outcome of sibling cells capable of killing and of being killed. The appropriate regulation of SigY appears to be essential for growth as evidenced by the inability to disrupt the gene for its putative antisigma. Our results confirm a role for SigY in antibiotic production and resistance, as has been found for other members of the extracytoplasmic function sigma factor family in B. subtilis, and shows that this role is achieved by affecting maintenance of the Spβ prophage.

  10. Live and let die - the B(sister MADS-box gene OsMADS29 controls the degeneration of cells in maternal tissues during seed development of rice (Oryza sativa.

    Xuelian Yang

    Full Text Available B(sister genes have been identified as the closest relatives of class B floral homeotic genes. Previous studies have shown that B(sister genes from eudicots are involved in cell differentiation during ovule and seed development. However, the complete function of B(sister genes in eudicots is masked by redundancy with other genes and little is known about the function of B(sister genes in monocots, and about the evolution of B(sister gene functions. Here we characterize OsMADS29, one of three MADS-box B(sister genes in rice. Our analyses show that OsMADS29 is expressed in female reproductive organs including the ovule, ovule vasculature, and the whole seed except for the outer layer cells of the pericarp. Knock-down of OsMADS29 by double-stranded RNA-mediated interference (RNAi results in shriveled and/or aborted seeds. Histological analyses of the abnormal seeds at 7 days after pollination (DAP indicate that the symplastic continuity, including the ovular vascular trace and the nucellar projection, which is the nutrient source for the filial tissue at early development stages, is affected. Moreover, degeneration of all the maternal tissues in the transgenic seeds, including the pericarp, ovular vascular trace, integuments, nucellar epidermis and nucellar projection, is blocked as compared to control plants. Our results suggest that OsMADS29 has important functions in seed development of rice by regulating cell degeneration of maternal tissues. Our findings provide important insights into the ancestral function of B(sister genes.

  11. An Atlas of Type I MADS Box Gene Expression during Female Gametophyte and Seed Development in Arabidopsis[W].

    Bemer, M.; Heijmans, K.; Airoldi, C.A.; Davies, B.; Angenent, G.C.

    2010-01-01

    Members of the plant type I MADS domain subfamily have been reported to be involved in reproductive development in Arabidopsis (Arabidopsis thaliana). However, from the 61 type I genes in the Arabidopsis genome, only PHERES1, AGAMOUS-LIKE80 (AGL80), DIANA, AGL62, and AGL23 have been functionally

  12. Towards an ESL design framework for adaptive and fault-tolerant MPSoCs: MADNESS or not?

    Cannella, E.; Di Gregorioi, L.; Fiorin, L.; Lindwer, M.; Meloni, P.; Neugebauer, O.; Pimentel, A.

    2011-01-01

    The MADNESS project aims at the definition of innovative system-level design methodologies for embedded MP-SoCs, extending the classic concept of design space exploration in multi-application domains to cope with high heterogeneity, technology scaling and system reliability. The main goal of the

  13. The contaminated Western Hero and other madness during the Atomic Age; Der verstrahlte Westernheld und anderer Irrsinn aus dem Atomzeitalter

    Herzog, Rudolph

    2012-11-01

    The book on mad activities during the Atomic Age includes the following topics: The second most hazardous invention in all time; the red bomb; the story on the tactic nuclear war; the contaminated Western hero or how the bomb came to Alaska; swords into plowshares; the apocalypse machine; flying reactors; how safe is safe?; atomic Australia; nuclear medicine on wrong ways; broken arrows.

  14. Genetic and epigenetic alteration among three homoeologous genes of a class E MADS box gene in hexaploid wheat.

    Shitsukawa, Naoki; Tahira, Chikako; Kassai, Ken-Ichiro; Hirabayashi, Chizuru; Shimizu, Tomoaki; Takumi, Shigeo; Mochida, Keiichi; Kawaura, Kanako; Ogihara, Yasunari; Murai, Koji

    2007-06-01

    Bread wheat (Triticum aestivum) is a hexaploid species with A, B, and D ancestral genomes. Most bread wheat genes are present in the genome as triplicated homoeologous genes (homoeologs) derived from the ancestral species. Here, we report that both genetic and epigenetic alterations have occurred in the homoeologs of a wheat class E MADS box gene. Two class E genes are identified in wheat, wheat SEPALLATA (WSEP) and wheat LEAFY HULL STERILE1 (WLHS1), which are homologs of Os MADS45 and Os MADS1 in rice (Oryza sativa), respectively. The three wheat homoeologs of WSEP showed similar genomic structures and expression profiles. By contrast, the three homoeologs of WLHS1 showed genetic and epigenetic alterations. The A genome WLHS1 homoeolog (WLHS1-A) had a structural alteration that contained a large novel sequence in place of the K domain sequence. A yeast two-hybrid analysis and a transgenic experiment indicated that the WLHS1-A protein had no apparent function. The B and D genome homoeologs, WLHS1-B and WLHS1-D, respectively, had an intact MADS box gene structure, but WLHS1-B was predominantly silenced by cytosine methylation. Consequently, of the three WLHS1 homoeologs, only WLHS1-D functions in hexaploid wheat. This is a situation where three homoeologs are differentially regulated by genetic and epigenetic mechanisms.

  15. Nutrition Knowledge of Teen-Agers.

    Skinner, Jean D.; Woodburn, Margy J.

    1984-01-01

    Nutrition knowledge tests were administered to 1,193 adolescents in Oregon prior to instructional units on nutrition in health and home economics classes. Mean scores on the tests were low. Guidelines for nutrition educators of adolescents are presented. (Author/CJB)

  16. Silvicultural madness

    Basnyat, Bijendra; Treue, Thorsten; Pokharel, R.K.

    2018-01-01

    Following a case study approach, this paper explains how scientific forest management plans were developed and implemented in community forests of a mid-hill district in Nepal. Field observations were carried over a period of two years (December 2014 to December 2016) in two community forests. User...... guidelines with little reference to the actual site quality, management objectives, and forest stand conditions. Apart from harvesting of trees, users hardly implemented the plans‘ silvicultural prescriptions and forest restoration activities. Moreover forest officials administratively reduced the number...... of trees that users could harvest to around half of what the plans allow. Accordingly, forest user groups face a paradoxical forest administration that promotes timber harvesting according to so-called scientific principles, which it then brushes aside to satisfy bureaucratic demands. The study concludes...

  17. Mascot Madness

    Henderson, Nancy

    2013-01-01

    For institutions around the world, mascots serve many purposes, such as raising school spirit and energizing sports teams. But mascots also can breed discord. In recent decades, some schools have been challenged to replace controversial Native American mascots while others have encountered resistance when introducing different mascot designs.…

  18. MADS specificity

    Mourik, van Hilda

    2017-01-01

    Encrypted in the DNA lays most information needed for the development of an organism. The transcription of this information into precise patterns of gene activity results in the development of different cell types, organs, and developmental structures. Moreover, transcriptional regulation enables

  19. El concepto de locura en la obra de Jacques Lacan The Concept Of Madness In The Jacques Lacan Work's

    Pablo D. Muñoz

    2008-12-01

    Full Text Available Desde sus primeros escritos Lacan distingue el concepto de locura del de psicosis, contradiciendo el uso común que los toma como equivalentes. Esta confusión produce efectos en la clínica psicoanalítica, por lo cual conviene aclarar y mantener su diferencia. Si bien los comentadores de su obra han reconocido esa distinción, no se ha destacado todo lo necesario la transformación que sufre el concepto de locura al final de la misma, ni se ha establecido con precisión su índole, sus alcances y consecuencias. Para hacerlo se propone explorar las diferencias entre locura y psicosis con el recurso de la teoría de nudos que Lacan emplea en dicho período, donde la psicosis es formalizada como una forma particular de anudamiento de los registros distinto del de la neurosis y la locura como su desanudamiento. En este trabajo se realiza un recorrido que enlaza las primeras referencias de Lacan sobre la locura con las recién mencionadas.First Lacan writings differentiates the "madness" concept from the other one of "psychosis", contradicting therefore the common use in language that takes them equivalent. This confusion can carry adverse effects in the psychoanalytic clinic. Thus is better to clarify and maintain its difference. Although Lacan comentators have recognized that distinction is not enough outstanded the transformation of the concept of "madness" at the his latest works, nor has settled down its nature, its reaches and consequences. In order to do it, we will explore the differences between "madness" and "psychosis" with his knots theory support, where the psychosis and neurosis like particular different form of knotting, and also defines "madness" like a registers untied. In this work is carried out a journey that connects the first references of Lacan about the madness with the last ones mentioned.

  20. Los sistemas de información geográfica (SIG una herramienta poderosa para la toma de decisiones

    Néstor Sáenz Saavedra

    1992-09-01

    Full Text Available Los sistemas de información tradicionales, orientados hacia el suministro de información útil para la toma de decisiones mediante la manipulación de una base de datos numérica, estaban impedidos para combinar simultáneamente los datos espaciales o gráficos 'Con sus datos descriptivos asociados. Esta labor de análisis se convertía entonces, en un proceso bastante complejo donde los cálculos geográficos debían realizarse por separado para, posteriormente buscar la manera de que los usuarios finales pudieran visualizar gráficamente los resultados tabulados sobre esquemas y mapas, en forma manual, o con la ayuda de programas de dibujo como el AUTOCAD. Esta tecnología, conocida con el nombre genérico de SISTEMAS DE INFORMACION GEOGRAFICO (SIG, se ha convertido en una poderosa herramienta al alcance de estudiantes y profesionales como ingenieros, geógrafos, médicos, historiadores y, en general, para todos los planificadores de aquellas disciplinas que necesitan información geográfica o georreferenciada, como ayuda para la solución de sus problemas. El presente documento pretende dar algunos conceptos generales sobre los SIG, su definición, componentes, datos de entrada y algunos procedimientos de análisis que se ofrecen con los paquetes disponibles en el mercado.

  1. Um framework para a avaliação de interfaces de aplicações SIG Web no dominio agricola

    Juliano Schimiguel

    2006-01-01

    Resumo: Sistemas de Informação Geográfica (SIG) são categorias de software que permitem a manipulação, gerenciamento e visualização de dados geo referenciados. O termo georeferenciado denota associação a um sistema de coordenadas geográficas. Existem inúmeras categorias de aplicações SIG. em diferentes escalas e domínios, abrangendo desde temas urbanos até ambientais. Aplicações de Sistemas de Informação Geográfica na Web, neste trabalho denominadas "aplicações SIG Web", são sistemas onde a '...

  2. Brugergenereret indhold

    Vistisen, Peter

    2010-01-01

    Måden, hvorpå forbrugerne kommunikerer og agerer på Internettet er via de sociale medier ændret markant. Spørgsmålet er, hvordan virksomheder og organisationer bedst muligt agerer i det brugergenererede sociale medielandskab. Skal man eksempelvis som ejer af et feriecenter overhovedet lade sig på...

  3. Accurate Numerical Simulations Of Chemical Phenomena Involved in Energy Production and Storage with MADNESS and MPQC: ALCF-2 Early Science Program Technical Report

    Vzquez-Mayagoitia, Alvaro [Argonne National Lab. (ANL), Argonne, IL (United States); Hammond, Jeff R. [Argonne National Lab. (ANL), Argonne, IL (United States)

    2013-09-16

    In order to solve the electronic structure of large molecular systems on petascale computers using MADNESS, a numerical tool kit, are required fast and accurate implementations for linear algebra. MADNESS uses multiresolution analysis and low separation rank which translates high dimensional functions in tensor products using Legendre polynomial. The multiple tensor products make to the singular value decomposition and matrix multiplication the most intense operations in MADNESS. This work discusses the interfacing of Eigen3 as a C++ substitute of LAPACK and introduces Elemental for the diagonalization of large matrices. Furthermore, the present paper shows the performance these libraries on Blue Gene/ Q.

  4. The Bacillus subtilis yaaH Gene Is Transcribed by SigE RNA Polymerase during Sporulation, and Its Product Is Involved in Germination of Spores

    Kodama, Takeko; Takamatsu, Hiromu; Asai, Kei; Kobayashi, Kazuo; Ogasawara, Naotake; Watabe, Kazuhito

    1999-01-01

    The expression of 21 novel genes located in the region from dnaA to abrB of the Bacillus subtilis chromosome was analyzed. One of the genes, yaaH, had a predicted promoter sequence conserved among SigE-dependent genes. Northern blot analysis revealed that yaaH mRNA was first detected from 2 h after the cessation of logarithmic growth (T2) of sporulation in wild-type cells and in spoIIIG (SigG−) and spoIVCB (SigK−) mutants but not in spoIIAC (SigF−) and spoIIGAB (SigE−) mutants. The transcription start point was determined by primer extension analysis; the −10 and −35 regions are very similar to the consensus sequences recognized by SigE-containing RNA polymerase. A YaaH-His tag fusion encoded by a plasmid with a predicted promoter for the yaaH gene was produced from T2 of sporulation in a B. subtilis transformant and extracted from mature spores, indicating that the yaaH gene product is a spore protein. Inactivation of the yaaH gene by insertion of an erythromycin resistance gene did not affect vegetative growth or spore resistance to heat, chloroform, and lysozyme. The germination of yaaH mutant spores in a mixture of l-asparagine, d-glucose, d-fructose, and potassium chloride was almost the same as that of wild-type spores, but the mutant spores were defective in l-alanine-stimulated germination. These results suggest that yaaH is a novel gene encoding a spore protein produced in the mother cell compartment from T2 of sporulation and that it is required for the l-alanine-stimulated germination pathway. PMID:10419957

  5. N-Acetylglutaminoyl-S-farnesyl-L-cysteine (SIG-1191): an anti-inflammatory molecule that increases the expression of the aquaglyceroporin, aquaporin-3, in human keratinocytes.

    Fernández, José R; Webb, Corey; Rouzard, Karl; Voronkov, Michael; Huber, Kristen L; Stock, Jeffry B; Stock, Maxwell; Gordon, Joel S; Perez, Eduardo

    2017-03-01

    Isoprenylcysteine (IPC) small molecules were discovered as signal transduction modulating compounds ~25 years ago. More recently, IPC molecules have demonstrated antioxidant and anti-inflammatory properties in a variety of dermal cells as well as antimicrobial activity, representing a novel class of compounds to ameliorate skin conditions and disease. Here, we demonstrate a new IPC compound, N-acetylglutaminoyl-S-farnesyl-L-cysteine (SIG-1191), which inhibits UVB-induced inflammation blocking pro-inflammatory cytokine interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) production. To investigate further the previously reported hydrating potential of IPC compounds, SIG-1191 was tested for its ability to modulate aquaporin expression. Specifically, aquaporin 3 (AQP3) the most abundant aquaporin found in skin has been reported to play a key role in skin hydration, elasticity and barrier repair. Results show here for the first time that SIG-1191 increases AQP3 expression in both cultured normal human epidermal keratinocytes as well as when applied topically in a three-dimensional (3D) reconstructed human skin equivalent. Additionally, SIG-1191 dose dependently increased AQP3 protein levels, as determined by specific antibody staining, in the epidermis of the 3D skin equivalents. To begin to elucidate which signaling pathways SIG-1191 may be modulating to increase AQP3 levels, we used several pharmacological pathway inhibitors and determined that AQP3 expression is mediated by the Mitogen-activated protein kinase/Extracellular signal-regulated kinase kinase (MEK) pathway. Altogether, these data suggest SIG-1191 represents a new IPC derivative with anti-inflammatory activity that may also promote increased skin hydration based on its ability to increase AQP3 levels.

  6. A perspective of Middle-Atmosphere Dynamics (MAD) studies at the New International Equatorial Observatory (NIEO)

    Yamanaka, M. D.; Fukao, S.

    1989-01-01

    The equatorial region has attracted many MAD studies mainly based on data of limited locations and resolutions. Established at NIEO are: (1) Climatology of the equatorial middle atmosphere (all of the mean zonal flow, the meridional and/or east-west circulations and the planetary/gravity waves are described based on massive, reliable data statistics); (2) Troposphere-stratosphere coupling at the equator (the candidate location of NIEO is just at the stratospheric fountain area where the tracers and waves are pumped up into the middle atmosphere); and (3) Mesosphere-thermosphere coupling at the equator; thermospheric superrotation, which may be caused either by ion drag or by tidal breaking, is examined in detail by observations covering a wide altitude range from the mesosphere through the thermosphere.

  7. MADNESS applied to density functional theory in chemistry and nuclear physics

    Fann, G I; Harrison, R J; Beylkin, G; Jia, J; Hartman-Baker, R; Shelton, W A; Sugiki, S

    2007-01-01

    We describe some recent mathematical results in constructing computational methods that lead to the development of fast and accurate multiresolution numerical methods for solving quantum chemistry and nuclear physics problems based on Density Functional Theory (DFT). Using low separation rank representations of functions and operators in conjunction with representations in multiwavelet bases, we developed a multiscale solution method for integral and differential equations and integral transforms. The Poisson equation, the Schrodinger equation, and the projector on the divergence free functions provide important examples with a wide range of applications in computational chemistry, nuclear physics, computational electromagnetic and fluid dynamics. We have implemented this approach along with adaptive representations of operators and functions in the multiwavelet basis and low separation rank (LSR) approximation of operators and functions. These methods have been realized and implemented in a software package called Multiresolution Adaptive Numerical Evaluation for Scientific Simulation (MADNESS)

  8. Mad Colonial Narrators in Anglo-Irish Literature: Lemuel Gulliver and Freddie Montgomery

    Patricia Jones

    2018-03-01

    Full Text Available The following discussion highlights parallels between the narrators, Lemuel Gulliver of Jonathan Swift’s Gulliver’s Travels (1726 and Freddie Montgomery of John Banville’s The Book of Evidence (1989. The argument calls on post-colonialism, Foucaultian theory of “will to truth” and the narrative theory of Shlomith Rimmon-Kenan to emphasize similarities in the rendering of mental degeneration in Gulliver and Montgomery. The colonial-induced mental breakdown of both narrators can be said to unravel, not so much in the tale these narrators think they are relating, but instead between the lines of their stories in narratives which continually focus attention back onto themselves. Despite the 260 years separating these works, the madness of both Gulliver and Montgomery can be interpreted as a reluctance on their respective parts to shed established colonial identities once the colonial stage has receded.

  9. Portfolio optimization using Mean Absolute Deviation (MAD and Conditional Value-at-Risk (CVaR

    Lucas Pelegrin da Silva

    Full Text Available Abstract This paper investigates the efficiency of traditional portfolio optimization models when the returns of financial assets are highly volatile, e.g., in financial crises periods. We also develop alternative optimization models that combine the mean absolute deviation (MAD and the conditional value at risk (CVaR, attempting to mitigate inefficient, low return and/or high-risk, portfolios. Three methodologies for estimating the probability of the asset’s historical returns are also compared. By using historical data on the Brazilian stock market between 2004 and 2013, we analyze the efficiency of the proposed approaches. Our results show that the traditional models provide portfolios with higher returns, but our propose model are able to generate lower risk portfolios, which might be more attractive in volatile markets. In addition, we find that models that do not use equiprobable scenarios produce better results in terms of return and risk.

  10. From morality to madness: a reappraisal of the asylum movement in psychiatry 1800-1940.

    Kosky, R

    1986-06-01

    This essay outlines the history of the asylum movement in psychiatry, but from a somewhat different angle than usual. It attempts to delineate the historical interactions between perceptions of morality and of madness. Changes in these interactions relate to the rise of the asylum movement, around 1800, and its demise, just after World War II. I argue that, whilst insanity was defined against the rational, secular morality of the eighteenth century, it could be separated from immorality and put aside into its asylum. Once mechanistic science and medical scientism began, during the nineteenth century, to include immorality in the systems of disease, the distinction could not hold. The asylums became flooded with the immoral, and management became custodial and nihilistic. This nexus was broken when the asylums were defined, by a few revolutionary superintendents, as instruments of social control. Nevertheless, intellectual paradigms derived from asylum psychiatry persist.

  11. The Overestimation Phenomenon in a Skill-Based Gaming Context: The Case of March Madness Pools.

    Kwak, Dae Hee

    2016-03-01

    Over 100 million people are estimated to take part in the NCAA Men's Basketball Tournament Championship bracket contests. However, relatively little is known about consumer behavior in skill-based gaming situations (e.g., sports betting). In two studies, we investigated the overestimation phenomenon in the "March Madness" context. In Study 1 (N = 81), we found that individuals who were allowed to make their own predictions were significantly more optimistic about their performance than individuals who did not make their own selections. In Study 2 (N = 197), all subjects participated in a mock competitive bracket pool. In line with the illusion of control theory, results showed that higher self-ratings of probability of winning significantly increased maximum willingness to wager but did not improve actual performance. Lastly, perceptions of high probability of winning significantly contributed to consumers' enjoyment and willingness to participate in a bracket pool in the future.

  12. From "Downton Abbey" to "Mad Men": TV series as the privileged format for transition eras

    Marie Maillos

    2016-06-01

    Full Text Available On each side of the Atlantic, the Downton Abbey and Mad Men shows have contributed to the transformation of the period drama genre on television: instead of being set in a single precise era, they take place over periods of historical transition, genuine intervals that are full of contrasts and confrontations and even propel the narrative forward. This new approach to time periods results as much from the TV series format as from its mass medium nature: on the one hand, these shows use the apparent invariability required by the serial form to reveal by contrast the transition at work and provide the narrative with the necessary conflict; on the other hand, through their depictions of transitional eras, they offer a commentary on our contemporary period, a characteristic device of television series. Therefore, both shows, notwithstanding their differences in themes, locations and craftmanship, play a part in making the transition period drama become a serial genre in its own right.

  13. New features of MadAnalysis 5 for analysis design and reinterpretation

    Conte, Eric; Fuks, Benjamin; Schmitt, Thibaut

    2015-01-01

    We present MadAnalysis 5, an analysis package dedicated to phenomenological studies of simulated collisions occurring in high-energy physics experiments. Within this framework, users are invited, through a user-friendly Python interpreter, to implement physics analyses in a very simple manner. A C++ code is then automatically generated, compiled and executed. Very recently, the expert mode of the program has been extended so that analyses with multiple signal/control regions can be handled. Additional observables have also been included, and an interface to several fast detector simulation packages has been developed, one of them being a tune of the Delphes 3 software. As a result, a recasting of existing ATLAS and CMS analyses can be achieved straightforwardly.

  14. Women at Home and Women in the Workplace in Matthew Weiner´s "Mad Men"

    Susana Sánchez Renieblas

    2013-01-01

    Full Text Available Matthew Weiner´s successful American TV series, Mad Men (2007, set in the 1960s in New York, unmasks the private and the public spaces of the home and the office. In these spaces, not only do the masculine protagonists interact, but also several feminine characters do as well. The three female characters (Betty, Peggy and Joan, who will be analyzed, represent the female stereotypes of this period: the idyllic housewife, the Sandra Dee prototype and the bombshell Marilyn Monroe archetype. In comparing the private and public spaces of the home and the office, these women´s sexuality and submission will be affected and influenced by the spaces they inhabit.

  15. Mad Speculation and Absolute Inhumanism: Lovecraft, Ligotti, and the Weirding of Philosophy

    Ben Woodard

    2011-03-01

    Full Text Available I want to propose, as a trajectory into the philosophically weird, an absurd theoretical claim and pursue it, or perhaps more accurately, construct it as I point to it, collecting the ground work behind me like the Perpetual Train from China Mieville's Iron Council which puts down track as it moves reclaiming it along the way. The strange trajectory is the following: Kant's critical philosophy and much of continental philosophy which has followed, has been a defense against horror and madness. Kant's prohibition on speculative metaphysics such as dogmatic metaphysics and transcendental realism, on thinking beyond the imposition of transcendental and moral constraints, has been challenged by numerous figures proceeding him.

  16. Philippe Pinel's "Memoir on Madness" of December 11, 1794: a fundamental text of modern psychiatry.

    Weiner, D B

    1992-06-01

    Philippe Pinel's "Memoir on Madness" can now be precisely dated. It was read to the Society for Natural History in Paris on Dec. 11, 1794, soon after the fall of the Jacobin dictatorship. It is thus a political document, an appeal to the Revolutionary government to build asylums where the mentally ill could be decently treated. It is translated here for the first time. Philippe Pinel (1745-1826) served as "physician of the infirmaries" at Bicêtre, the public hospice for men near Paris, from 1793 to 1795. In the "Memoir on Madness" he explains his "psychologic treatment," the principles of the humane method that made him the founder of psychiatry in France. Pinel states that mental illness is often curable. To arrive at a diagnosis, the physician must carefully observe a patient's behavior, interview him, listen carefully, and take notes. He must understand the natural history of the disease and the precipitating event and write an accurate case history. Diagnosis and prognosis can then be made. Periodic patterns of mental illness can be helpful for therapy. Usually only one faculty is affected. Patients with delusions may be malicious or murderous. They may have to be restrained; it was Pinel's assistant, Jean Baptiste Pussin, who removed the chains from the insane men at Bicêtre Hospice in 1797 and replaced them with strait-jackets. Pinel followed suit at the Salpêtrière, the public hospice for women, 3 years later. Pinel here states that one must "dominate agitated madmen while respecting human rights."

  17. La locura y sus versiones en la obra de J. Lacan II: locura y psicosis The madness and his versions in the J. Lacan work's II: madness and psychosis

    Pablo D. Muñoz

    2010-12-01

    Full Text Available Este trabajo presenta parte de las conclusiones del proyecto de investigación UBACyT P601: "Variaciones del concepto de locura en la obra de J. Lacan. Su incidencia en el diagnóstico diferencial neurosis-psicosis", aprobado y financiado para el período 2008-2010. Se aborda especialmente la cuestión problemática de su delimitación conceptual en la obra de Lacan, en la cual el término locura es empleado de diversos modos. Esta variedad se plasma en su articulación con distintos conceptos y con problemas clínicos de diversa índole. La presentación conjunta de estos usos permite obtener una visión bastante amplia de las ventajas que trae servirse de alguna de las versiones de la locura que se presentan en la obra de Lacan. En este trabajo se aplican a la articulación locura-psicosis, abordando no sólo la problemática conceptual en juego en lo atinente a alcanzar una definición más precisa de "locura", sino sobre todo el agudo problema clínico que presentan lo que podríamos designar "psicosis enloquecidas".This paper presents some of the conclusions of the research project UBACyT P601: "Variations of the concept of madness in the work of J. Lacan. Its incidence in the differential diagnosis neurosis psychosis ", approved and funded for the period 2008-2010. It especially addresses the problematic issue of its conceptual delimitation in the Lacan work, in which the term "madness" is used in several senses. This variety is reflected in its theoretical concept articulation and several clinical issues. The whole presentation of these work allows a wider vision of the benefits that brings to use any of the versions of "madness" concept that arise in the work of Lacan. This work apply to the madness-psychosis articulation, addressing not only the conceptual issues, but also achieving a more precise definition of "madness"; and above of all it takes the big clinical problem in what we might call "mad psychosis".

  18. La locura y sus versiones en la obra de J. Lacan: I: locura y neurosis The madness and his versions in the J. Lacan work's: I: madness and neurosis

    Pablo D. Muñoz

    2010-12-01

    Full Text Available Este trabajo presenta parte de las conclusiones del proyecto de investigación UBACyT P601: "Variaciones del concepto de locura en la obra de J. Lacan. Su incidencia en el diagnóstico diferencial neurosis-psicosis", aprobado y financiado para el período 2008-2010. Se aborda especialmente la cuestión problemática de su delimitación conceptual en la obra de Lacan, en la cual el término locura es empleado de diversos modos. Esta variedad se plasma en su articulación con distintos conceptos y con problemas clínicos de diversa índole. La presentación conjunta de estos usos permite obtener una visión bastante amplia de las ventajas que trae servirse de alguna de las versiones de la locura que se presentan en la obra de Lacan. En este trabajo se aplican a la articulación locura-neurosis, abordando no sólo la problemática conceptual en juego en lo atinente a alcanzar una definición más precisa de "locura", sino sobre todo el agudo problema clínico que presentan lo que podríamos designar "neurosis enloquecidas".This paper presents some of the conclusions of the research project UBACyT P601: "Variations of the concept of madness in the work of J. Lacan. Its incidence in the differential diagnosis neurosis psychosis ", approved and funded for the period 2008-2010. It especially addresses the problematic issue of its conceptual delimitation in the Lacan work, in which the term "madness" is used in several senses. This variety is reflected in its theoretical concept articulation and several clinical issues. The whole presentation of these work allows a wider vision of the benefits that brings to use any of the versions of "madness" concept that arise in the work of Lacan. This work apply to the madness-neurosis articulation, addressing not only the conceptual issues, but also achieving a more precise definition of "madness"; and above of all it takes the big clinical problem in what we might call "mad neurosis".

  19. Discrimination of solvent from protein regions in native Fouriers as a means of evaluating heavy-atom solutions in the MIR and MAD methods

    Terwilliger, Thomas C.; Berendzen, Joel

    1999-01-01

    The presence of distinct regions of high and low density variation in electron-density maps is found to be a good indicator of the correctness of a heavy-atom solution in the MIR and MAD methods. An automated examination of the native Fourier is tested as a means of evaluation of a heavy-atom solution in MAD and MIR methods for macromolecular crystallography. It is found that the presence of distinct regions of high and low density variation in electron-density maps is a good indicator of the correctness of a heavy-atom solution in the MIR and MAD methods. The method can be used to evaluate heavy-atom solutions during MAD and MIR structure solutions and to determine the handedness of the structure if anomalous data have been measured

  20. Transcriptional regulation of the operon encoding stress-responsive ECF sigma factor SigH and its anti-sigma factor RshA, and control of its regulatory network in Corynebacterium glutamicum.

    Busche, Tobias; Silar, Radoslav; Pičmanová, Martina; Pátek, Miroslav; Kalinowski, Jörn

    2012-09-03

    The expression of genes in Corynebacterium glutamicum, a Gram-positive non-pathogenic bacterium used mainly for the industrial production of amino acids, is regulated by seven different sigma factors of RNA polymerase, including the stress-responsive ECF-sigma factor SigH. The sigH gene is located in a gene cluster together with the rshA gene, putatively encoding an anti-sigma factor. The aim of this study was to analyze the transcriptional regulation of the sigH and rshA gene cluster and the effects of RshA on the SigH regulon, in order to refine the model describing the role of SigH and RshA during stress response. Transcription analyses revealed that the sigH gene and rshA gene are cotranscribed from four sigH housekeeping promoters in C. glutamicum. In addition, a SigH-controlled rshA promoter was found to only drive the transcription of the rshA gene. To test the role of the putative anti-sigma factor gene rshA under normal growth conditions, a C. glutamicum rshA deletion strain was constructed and used for genome-wide transcription profiling with DNA microarrays. In total, 83 genes organized in 61 putative transcriptional units, including those previously detected using sigH mutant strains, exhibited increased transcript levels in the rshA deletion mutant compared to its parental strain. The genes encoding proteins related to disulphide stress response, heat stress proteins, components of the SOS-response to DNA damage and proteasome components were the most markedly upregulated gene groups. Altogether six SigH-dependent promoters upstream of the identified genes were determined by primer extension and a refined consensus promoter consisting of 45 original promoter sequences was constructed. The rshA gene codes for an anti-sigma factor controlling the function of the stress-responsive sigma factor SigH in C. glutamicum. Transcription of rshA from a SigH-dependent promoter may serve to quickly shutdown the SigH-dependent stress response after the cells have

  1. Transcriptional regulation of the operon encoding stress-responsive ECF sigma factor SigH and its anti-sigma factor RshA, and control of its regulatory network in Corynebacterium glutamicum

    Busche Tobias

    2012-09-01

    Full Text Available Abstract Background The expression of genes in Corynebacterium glutamicum, a Gram-positive non-pathogenic bacterium used mainly for the industrial production of amino acids, is regulated by seven different sigma factors of RNA polymerase, including the stress-responsive ECF-sigma factor SigH. The sigH gene is located in a gene cluster together with the rshA gene, putatively encoding an anti-sigma factor. The aim of this study was to analyze the transcriptional regulation of the sigH and rshA gene cluster and the effects of RshA on the SigH regulon, in order to refine the model describing the role of SigH and RshA during stress response. Results Transcription analyses revealed that the sigH gene and rshA gene are cotranscribed from four sigH housekeeping promoters in C. glutamicum. In addition, a SigH-controlled rshA promoter was found to only drive the transcription of the rshA gene. To test the role of the putative anti-sigma factor gene rshA under normal growth conditions, a C. glutamicum rshA deletion strain was constructed and used for genome-wide transcription profiling with DNA microarrays. In total, 83 genes organized in 61 putative transcriptional units, including those previously detected using sigH mutant strains, exhibited increased transcript levels in the rshA deletion mutant compared to its parental strain. The genes encoding proteins related to disulphide stress response, heat stress proteins, components of the SOS-response to DNA damage and proteasome components were the most markedly upregulated gene groups. Altogether six SigH-dependent promoters upstream of the identified genes were determined by primer extension and a refined consensus promoter consisting of 45 original promoter sequences was constructed. Conclusions The rshA gene codes for an anti-sigma factor controlling the function of the stress-responsive sigma factor SigH in C. glutamicum. Transcription of rshA from a SigH-dependent promoter may serve to quickly

  2. “You can’t be a man. Be a woman, it’s a powerful business when done correctly”:women and work in Mad Men

    Korhonen, M. (Maria)

    2016-01-01

    Abstract The topic of this thesis pertains to how female characters are portrayed in the American period drama TV series Mad Men (2007–2015) that is set in the 1960s, and the main focus is on Margaret ‘Peggy’ Olson, who works as a copywriter at the fictional advertising agency Sterling Cooper. This thesis focuses on three main themes through which Peggy Olson’s journey in Mad Men are analyzed: appearance, obstacles in the w...

  3. MadR1, a Mycobacterium tuberculosis cell cycle stress response protein that is a member of a widely conserved protein class of prokaryotic, eukaryotic and archeal origin.

    Crew, Rebecca; Ramirez, Melissa V; England, Kathleen; Slayden, Richard A

    2015-05-01

    Stress-induced molecular programs designed to stall division progression are nearly ubiquitous in bacteria, with one well-known example being the participation of the SulA septum inhibiting protein in the SOS DNA damage repair response. Mycobacteria similarly demonstrate stress-altered growth kinetics, however no such regulators have been found in these organisms. We therefore set out to identify SulA-like regulatory proteins in Mycobacterium tuberculosis. A bioinformatics modeling-based approach led to the identification of rv2216 as encoding for a protein with weak similarity to SulA, further analysis distinguished this protein as belonging to a group of uncharacterized growth promoting proteins. We have named the mycobacterial protein encoded by rv2216 morphology altering division regulator protein 1, MadR1. Overexpression of madR1 modulated cell length while maintaining growth kinetics similar to wild-type, and increased the proportion of bent or V-form cells in the population. The presence of MadR1-GFP at regions of cellular elongation (poles) and morphological differentiation (V-form) suggests MadR1 involvement in phenotypic heterogeneity and longitudinal cellular growth. Global transcriptional analysis indicated that MadR1 functionality is linked to lipid editing programs required for growth and persistence. This is the first report to differentiate the larger class of these conserved proteins from SulA proteins and characterizes MadR1 effects on the mycobacterial cell. Copyright © 2015 Elsevier Ltd. All rights reserved.

  4. Bildskapande i relation till genus i förskolans verksamhet : Att skapa sig själv och genus

    Brännlund, Angelika

    2018-01-01

    Studiens syfte är att bidra till ökad medvetenhet för verksamma inom förskolans område kring frågor som rör pedagogers förhållningssätt kring relationen mellan bildskapande och genus, men även hur dessa förhållningssätt kan påverka barnens möjligheter i deras bildskapande. En viktig fråga som burits med från starten av arbetet och som lyser igenom i resultatdelen har varit: Vilka möjligheter att förhålla sig till bildskapande och genus kan uppstå om ett kritiskt sätt att tänka kring könsstere...

  5. Regionalización hidrogeológica de la provincia Pinar del Río en un SIG

    Nelson González-Cabrera

    2004-03-01

    Full Text Available Se presenta la regionalización hidrogeológica de la provincia Pinar del Río en plataforma SIG. Los principios de elaboración de este mapa proponen la división del territorio a partir de la integración automatizada de un conjunto de variables naturales, tales como: características físico-geográficas, hidrología, geomorfología, hidrogeología y particularidades estructuro-faciales, considerando el reconocimiento de las regularidades regionales y los rasgos comunes de cada estructura hidrogeológica, entre las que se pueden citar: las fuentes de formación de las aguas subterráneas, condiciones de alimentación, tránsito y descarga de los horizontes y complejos acuíferos, y zonalidades hidroquímica e hidrodinámica.

  6. Impact of growth conditions and role of sigB on Listeria monocytogenes fitness in single and mixed biofilms cultured with Lactobacillus plantarum

    Saa Ibusquiza, P.; Nierop Groot, M.N.; Deban Valles, A.; Abee, T.; Besten, den H.M.W.

    2015-01-01

    The role of sigB, a major transcriptional regulator of stress response genes, was assessed in formation of single and mixed species biofilms of Listeria monocytogenes EGD-e and Lactobacillus plantarum WCFS1 as secondary species at 20 °C and 30 °C using different medium compositions (nutrient-rich

  7. Closure Report for Corrective Action Unit 254: Area 25, R-MAD Decontamination Facility, Nevada Test Site, Nevada

    G. N. Doyle

    2002-02-01

    Corrective Action Unit (CAU) 254 is located in Area 25 of the Nevada Test Site (NTS), approximately 100 kilometers (km) (62 miles) northwest of Las Vegas, Nevada. The site is located within the Reactor Maintenance, Assembly and Disassembly (R-MAD) compound and consists of Building 3126, two outdoor decontamination pads, and surrounding areas within an existing fenced area measuring approximately 50 x 37 meters (160 x 120 feet). The site was used from the early 1960s to the early 1970s as part of the Nuclear Rocket Development Station program to decontaminate test-car hardware and tooling. The site was reactivated in the early 1980s to decontaminate a radiologically contaminated military tank. This Closure Report (CR) describes the closure activities performed to allow un-restricted release of the R-MAD Decontamination Facility.

  8. MAdCAM-1 is needed for diabetes development mediated by the T cell clone, BDC-2·5

    Phillips, Jenny M; Haskins, Kathryn; Cooke, Anne

    2005-01-01

    The NOD-derived islet-reactive CD4+ T cell clone, BDC-2·5, is able to transfer diabetes to neonatal non-obese diabetic (NOD) mice but is unable to transfer disease to either adult NOD or NOD scid recipients. Transfer of diabetes to adult recipients by BDC-2·5 is only accomplished by cotransfer of CD8+ T cells from a diabetic donor. To understand why this CD4+ T cell clone is able to mediate diabetes in neonatal but not the adult recipients we examined the ability of the clone to traffic in the different recipients. Our studies showed that MAdCAM-1 has a very different expression pattern in the neonatal and adult pancreas. Blockade of this addressin prevents the clone from transferring diabetes to neonatal mice, suggesting that the differential pancreatic expression of MAdCAM-1 in neonatal and adult pancreas provides an explanation of the differences in diabetes development. PMID:16313366

  9. Constructed identities A Chronotopic reading of The Great Gatsby, The Man in the Gray Flannel Suit, and Mad Men

    Yndestad, Ingrid Rivedal

    2016-01-01

    This thesis aims to explore how the past figures in F. Scott Fitzgerald's The Great Gatsby (1925), Sloan Wilson's The Man in the Gray Flannel Suit (1955), and in the AMC series Mad Men (2007-2015), written by Matthew Weiner. Focusing on the main protagonists in these works, namely Jay Gatsby, Thomas Rath, and Donald Draper, this thesis examines how the past makes itself valid in these characters' present lives and how it arguably affects their future lives. In do...

  10. The extracytoplasmic function sigma factor SigV plays a key role in the original model of lysozyme resistance and virulence of Enterococcus faecalis.

    André Le Jeune

    Full Text Available BACKGROUND: Enterococcus faecalis is one of the leading agents of nosocomial infections. To cause diseases, pathogens or opportunistic bacteria have to adapt and survive to the defense systems encountered in the host. One of the most important compounds of the host innate defense response against invading microorganisms is lysozyme. It is found in a wide variety of body fluids, as well as in cells of the innate immune system. Lysozyme could act either as a muramidase and/or as a cationic antimicrobial peptide. Like Staphylococcus aureus, E. faecalis is one of the few bacteria that are completely lysozyme resistant. RESULTS: This study revealed that oatA (O-acetyl transferase and dlt (D-Alanylation of lipoteicoic acids genes contribute only partly to the lysozyme resistance of E. faecalis and that a specific transcriptional regulator, the extracytoplasmic function SigV sigma factor plays a key role in this event. Indeed, the sigV single mutant is as sensitive as the oatA/dltA double mutant, and the sigV/oatA/dltA triple mutant displays the highest level of lysozyme sensitivity suggesting synergistic effects of these genes. In S. aureus, mutation of both oatA and dlt genes abolishes completely the lysozyme resistance, whereas this is not the case in E. faecalis. Interestingly SigV does not control neither oatA nor dlt genes. Moreover, the sigV mutants clearly showed a reduced capacity to colonize host tissues, as they are significantly less recovered than the parental JH2-2 strain from organs of mice subjected to intravenous or urinary tract infections. CONCLUSIONS: This work led to the discovery of an original model of lysozyme resistance mechanism which is obviously more complex than those described for other Gram positive pathogens. Moreover, our data provide evidences for a direct link between lysozyme resistance and virulence of E. faecalis.

  11. Novel complex MAD phasing and RNase H structural insights using selenium oligonucleotides

    Abdur, Rob; Gerlits, Oksana O.; Gan, Jianhua; Jiang, Jiansheng; Salon, Jozef; Kovalevsky, Andrey Y.; Chumanevich, Alexander A.; Weber, Irene T.; Huang, Zhen, E-mail: huang@gsu.edu [Georgia State University, Atlanta, GA 30303 (United States)

    2014-02-01

    Selenium-derivatized oligonucleotides may facilitate phase determination and high-resolution structure determination for protein–nucleic acid crystallography. The Se atom-specific mutagenesis (SAM) strategy may also enhance the study of nuclease catalysis. The crystal structures of protein–nucleic acid complexes are commonly determined using selenium-derivatized proteins via MAD or SAD phasing. Here, the first protein–nucleic acid complex structure determined using selenium-derivatized nucleic acids is reported. The RNase H–RNA/DNA complex is used as an example to demonstrate the proof of principle. The high-resolution crystal structure indicates that this selenium replacement results in a local subtle unwinding of the RNA/DNA substrate duplex, thereby shifting the RNA scissile phosphate closer to the transition state of the enzyme-catalyzed reaction. It was also observed that the scissile phosphate forms a hydrogen bond to the water nucleophile and helps to position the water molecule in the structure. Consistently, it was discovered that the substitution of a single O atom by a Se atom in a guide DNA sequence can largely accelerate RNase H catalysis. These structural and catalytic studies shed new light on the guide-dependent RNA cleavage.

  12. Mad Cows and CJD A Physicist's View of Prion Brain Diseases

    Morrison, Douglas Robert Ogston

    1997-01-01

    The research of Carleton Gajdusek on a stone-age tribe in Papua New Guinea, who suffered from a mysterious disease, kuru, spread by cannibalism, is described and short extracts from his films will be shown. Some deaths from kuru are still occuring after 45 years. This disease is believed to be caused by an entirely new mechanism, not a virus or bacteria, but by a small molecule known as a prion that occurs naturally in many living forms. The Prion Only hypothesis of Stan Prusiner is discussed critically. It has been calculated that 900,000 cows in Britain had the Mad Cow disease, BSE, but most were slaughtered before symptoms were recognised. This epidemic started with 10 cases in the first year, and finally 160,000 were officially classified as having BSE; it is now slowly dying out. The human epidemic, caused by a new version of Creutzfeldt-Jakob Disease, nvCJD, affects mainly young people, has just begun with 10 cases in the first year. The average incubation time may be about 14 years then death follows i...

  13. Method and Madness: An Analysis of Edgar Allan Poe's “The Bells”

    U. Marie Engemann

    2017-11-01

    Full Text Available In this article, U. Marie Engemann has tried to indicate the method and internal cohesion of “The Bells”: a poem by Edgar Allan Poe, regardless of its apparent madness. This poem consists of four stanzas each of which is a symbol of a stage in the human's life for which a specific bell has been dedicated to. This poem follows trochaic meter in which the poet ingeniously has used catalexis in order to imitate the chime of bells. Throughout the poem, he has used different sound devices such as rhyme, sound patterning, alliteration, and onomatopoeia to create parallelism, and accordingly, he has conformed the chime of the bells to the dominant feature of each stage of life; thus, a meaningful cohesion has been created. This article can show how a poet may use different sound devices of his own language to create a parallelism between sound symbolism and meaning. The structure of the article has been changed a little by inserting the figures and the tables in the appendices into its straight text in translation so that it becomes easier for the readers to make a link between discussions and the data presented in each figure or table.

  14. Perspectives on MADS-box expression during orchid flower evolution and development.

    Mondragón-Palomino, Mariana

    2013-01-01

    The diverse morphology of orchid flowers and their complex, often deceptive strategies to become pollinated have fascinated researchers for a long time. However, it was not until the 20th century that the ontogeny of orchid flowers, the genetic basis of their morphology and the complex phylogeny of Orchidaceae were investigated. In parallel, the improvement of techniques for in vitro seed germination and tissue culture, together with studies on biochemistry, physiology, and cytology supported the progress of what is now a highly productive industry of orchid breeding and propagation. In the present century both basic research in orchid flower evo-devo and the interest for generating novel horticultural varieties have driven the characterization of many members of the MADS-box family encoding key regulators of flower development. This perspective summarizes the picture emerging from these studies and discusses the advantages and limitations of the comparative strategy employed so far. I address the growing role of natural and horticultural mutants in these studies and the emergence of several model species in orchid evo-devo and genomics. In this context, I make a plea for an increasingly integrative approach.

  15. Perspectives on MADS-box expression during orchid flower evolution and development

    Mariana eMondragón Palomino

    2013-09-01

    Full Text Available The diverse morphology of orchid flowers and their complex, often deceptive strategies to become pollinated have fascinated researchers for a long time. However, it was not until the 20th century that the ontogeny of orchid flowers, the genetic basis of their morphology and the complex phylogeny of Orchidaceae were investigated. In parallel, the improvement of techniques for in vitro seed germination and tissue culture, together with studies on biochemistry, physiology and cytology supported the progress of what is now a highly productive industry of orchid breeding and propagation. In the present century both basic research in orchid flower evo-devo and the interest for generating novel horticultural varieties have driven the characterization of many members of the MADS-box family encoding key regulators of flower development. This perspective summarizes the picture emerging from these studies and discusses the advantages and limitations of the comparative strategy employed so far. I address the growing role of natural and horticultural mutants in these studies and the emergence of several model species in orchid evo-devo and genomics. In this context, I make a plea for an increasingly integrative approach.

  16. A comparison of prognostic significance of strong ion gap (SIG) with other acid-base markers in the critically ill: a cohort study.

    Ho, Kwok M; Lan, Norris S H; Williams, Teresa A; Harahsheh, Yusra; Chapman, Andrew R; Dobb, Geoffrey J; Magder, Sheldon

    2016-01-01

    This cohort study compared the prognostic significance of strong ion gap (SIG) with other acid-base markers in the critically ill. The relationships between SIG, lactate, anion gap (AG), anion gap albumin-corrected (AG-corrected), base excess or strong ion difference-effective (SIDe), all obtained within the first hour of intensive care unit (ICU) admission, and the hospital mortality of 6878 patients were analysed. The prognostic significance of each acid-base marker, both alone and in combination with the Admission Mortality Prediction Model (MPM0 III) predicted mortality, were assessed by the area under the receiver operating characteristic curve (AUROC). Of the 6878 patients included in the study, 924 patients (13.4 %) died after ICU admission. Except for plasma chloride concentrations, all acid-base markers were significantly different between the survivors and non-survivors. SIG (with lactate: AUROC 0.631, confidence interval [CI] 0.611-0.652; without lactate: AUROC 0.521, 95 % CI 0.500-0.542) only had a modest ability to predict hospital mortality, and this was no better than using lactate concentration alone (AUROC 0.701, 95 % 0.682-0.721). Adding AG-corrected or SIG to a combination of lactate and MPM0 III predicted risks also did not substantially improve the latter's ability to differentiate between survivors and non-survivors. Arterial lactate concentrations explained about 11 % of the variability in the observed mortality, and it was more important than SIG (0.6 %) and SIDe (0.9 %) in predicting hospital mortality after adjusting for MPM0 III predicted risks. Lactate remained as the strongest predictor for mortality in a sensitivity multivariate analysis, allowing for non-linearity of all acid-base markers. The prognostic significance of SIG was modest and inferior to arterial lactate concentration for the critically ill. Lactate concentration should always be considered regardless whether physiological, base excess or physical-chemical approach

  17. Proyecciones Vintage: Mad Men y Cuéntame cómo pasó / Vintage Projections: Mad Men and Cuéntame cómo pasó

    Laura García Pousa

    2016-09-01

    Full Text Available Las series de televisión han puesto a prueba la capacitación del drama mediante la construcción de un discurso propio y diferenciado, que las convierte en uno de los referentes artísticos contemporáneos de mayor expansión. En este artículo acudimos a su estudio específico como nuevos artefactos culturales que trabajan con múltiples realidades y niveles narrativos creando ficciones capaces de aglutinar éxito comercial y nuevas parcelas audiovisuales que generan reflexiones sobre la representación de la memoria histórica. Aunque distantes en su concepción, desarrollo, producción y tratamiento, nos centramos en Mad Men (AMC: 2007- y en Cuéntame cómo pasó (Grupo Ganga: 2001- por su importante trabajo de contextualización dramática donde la cultura popular, la visión mediatizada y los mitos se convierten en constantes de sus narraciones. El estudio comparativo de dos nudos dramáticos como son el asesinato de John F. Kennedy en Mad Men y el atentado contra Carrero Blanco en Cuéntame cómo pasó nos permitirá iniciar y profundizar en las formas argumentales, discursivas y estéticas de ambas producciones dentro de su desarrollo en continuidad como obras de ficción de largo recorrido.Palabras clave: series de televisión, ficción, memoria, historia, representación, Mad Men, Cuéntame cómo pasó, cultura popular, identidad.AbstractTelevision series have challenged the drama by constructing a personal and differentiated discourse, turning it into one of the contemporary artistic genres experiencing the greatest expansion at the moment. In this article, we’ve undertaken the study of such series as new cultural artifacts that work with multiple realities and narrative levels, creating fictions that garner commercial success, and new audiovisual parcels that generate reflections on the representations of historical memory. Although distinct in their conception, development, production and material, we will focus on the series Mad

  18. Infortunios de Alonso Ramírez, de Carlos de Sigüenza y Góngora: aproximaciones a una geografía poscolonial

    Mariana Zinni

    2014-06-01

    Infortunios de Alonso Ramírez can be read as a mimetic exercise related to some core problems regarding the dynamics of colonial domination. Such exercise not only mimes-imitates textual aspects of other genres by reshaping the process of text constitution itself (picaresque novel, travelogue, but also inscribes itself in the relationships between colonized and colonizers. I propose a reading of this text by arguing that Sigüenza y Góngora, while re-presenting the colony vis-à-vis the metropolis, situates Mexico City in a cultural circuit and proposes a movement of “metropolization” of the colonial city regarding the other periphery, the Philippines. Consequently, Sigüenza will descenter the empire by establishing the existence of this other suburb in a clever stroke of which I will propose to call “postcolonial geography”.

  19. SigEMD: A powerful method for differential gene expression analysis in single-cell RNA sequencing data.

    Wang, Tianyu; Nabavi, Sheida

    2018-04-24

    Differential gene expression analysis is one of the significant efforts in single cell RNA sequencing (scRNAseq) analysis to discover the specific changes in expression levels of individual cell types. Since scRNAseq exhibits multimodality, large amounts of zero counts, and sparsity, it is different from the traditional bulk RNA sequencing (RNAseq) data. The new challenges of scRNAseq data promote the development of new methods for identifying differentially expressed (DE) genes. In this study, we proposed a new method, SigEMD, that combines a data imputation approach, a logistic regression model and a nonparametric method based on the Earth Mover's Distance, to precisely and efficiently identify DE genes in scRNAseq data. The regression model and data imputation are used to reduce the impact of large amounts of zero counts, and the nonparametric method is used to improve the sensitivity of detecting DE genes from multimodal scRNAseq data. By additionally employing gene interaction network information to adjust the final states of DE genes, we further reduce the false positives of calling DE genes. We used simulated datasets and real datasets to evaluate the detection accuracy of the proposed method and to compare its performance with those of other differential expression analysis methods. Results indicate that the proposed method has an overall powerful performance in terms of precision in detection, sensitivity, and specificity. Copyright © 2018 Elsevier Inc. All rights reserved.

  20. Sistemas agroforestales como estrategia para el manejo de ecosistemas de Bosque seco Tropical en el suroccidente colombiano utilizando los sig

    Nathaly de los Ángeles Mazo

    2016-01-01

    Full Text Available Los sistemas agroforestales son una alternativa para el manejo de zonas de bosque seco tropical de laderas intervenidas o en degradación. Sus beneficios se basan en el uso eficiente de los recursos, la productividad y la seguridad alimentaria de las comunidades rurales. Localizar lugares más aptos para dichos sistemas es un requisito en el proceso de extensión agrícola. Con este propósito, la utilización de modelos probabilísticos integrados con Sistemas de Información Geográfica (SIG permite identificar aquellas áreas potenciales para la implementación de nuevas tecnologías, de modo similar a la identificación de nichos de especies en estudios de biodiversidad. Siguiendo este enfoque, se utilizó el peso de la evidencia y la regresión logística para generar superficies indicativas de áreas adecuadas para implementar la agroforestería en el suroccidente colombiano.

  1. Use of SIG device to accurately place permanent miniature dental implants to retain mandibular overdenture. A case report.

    Sussman, Harold I; Goodridge, Opal F

    2006-01-01

    A case of mini-dental implant insertion for retention of a mandibular overdenture in a hospitalized patient has been documented. The additional use of the SIG (drill guide) directional device in the implant placement protocol gave the practitioner more confidence and resulted in the proper alignment of the three ball-top, one-piece fixtures. The three implants were inserted exactly 1 cm apart and parallel to each other. The distal fixtures were approximately 1 cm away from the mental foramina, thereby eliminating the risk of lip paresthesia. Keeper caps were placed in the denture's intaglio after one month. The keeper caps allowed for proper retention of the overdenture. The caps also enabled the patient to easily insert and withdraw his denture, even though he displayed limited manual dexterity. The tissue response was excellent, and oral hygiene was made easier with adequate spacing of the exposed ball-tops. The overall experience for both the operator and the patient was very positive. General dentists should be able to readily master this technique and add it to their armamentarium for the benefit of all their patients.

  2. Sistemas agroforestales como estrategia para el manejo de ecosistemas de Bosque seco Tropical en el suroccidente colombiano utilizando los SIG

    Nathaly de los Ángeles Mazo

    2016-01-01

    Full Text Available Los sistemas agroforestales son una alternativa para el manejo de zonas de bosque seco tropical de laderas intervenidas o en degradación. Sus beneficios se basan en el uso eficiente de los recursos, la productividad y la seguridad alimentaria de las comunidades rurales. Localizar lugares más aptos para dichos sistemas es un requisito en el proceso de extensión agrícola. Con este propósito, la utilización de modelos probabilísticos integrados con Sistemas de Información Geográfica (SIG permite identificar aquellas áreas potenciales para la implementación de nuevas tecnologías, de modo similar a la identificación de nichos de especies en estudios de biodiversidad. Siguiendo este enfoque, se utilizó el peso de la evidencia y la regresión logística para generar superficies indicativas de áreas adecuadas para implementar la agroforestería en el suroccidente colombiano.

  3. 44 Apport du SIG et de la télédétection dans la modélisation spatiale ...

    Unicornis

    44. ISSN 1813-548X, http://www.afriquescience.info. Ahmed Nasreddine El FAHCHOUCH et al. Apport du SIG et de la télédétection dans la modélisation spatiale de la susceptibilité aux mouvements de terrain dans la région d'Al Hoceima,. Rif Oriental, Maroc. Ahmed NasreddineEl FAHCHOUCH*, Lahsen AIT BRAHIM, ...

  4. Seasonal and spatial variations in fish and macrocrustacean assemblage structure in Mad Island Marsh estuary, Texas

    Akin, S.; Winemiller, K. O.; Gelwick, F. P.

    2003-05-01

    Fish and macrocrustacean assemblage structure was analyzed along an estuarine gradient at Mad Island Marsh (MIM), Matagorda Bay, TX, during March 1998-August 1999. Eight estuarine-dependent fish species accounted for 94% of the individual fishes collected, and three species accounted for 96% of macrocrustacean abundance. Consistent with evidence from other Gulf of Mexico estuarine studies, species richness and abundance were highest during late spring and summer, and lowest during winter and early spring. Sites near the bay supported the most individuals and species. Associations between fish abundance and environmental variables were examined with canonical correspondence analysis. The dominant gradient was associated with water depth and distance from the bay. The secondary gradient reflected seasonal variation and was associated with temperature, salinity, dissolved oxygen, and vegetation cover. At the scales examined, estuarine biota responded to seasonal variation more than spatial variation. Estuarine-dependent species dominated the fauna and were common throughout the open waters of the shallow lake during winter-early spring when water temperature and salinity were low and dissolved oxygen high. During summer-early fall, sub-optimal environmental conditions (high temperature, low DO) in upper reaches accounted for strong spatial variation in assemblage composition. Small estuarine-resident fishes and the blue crab ( Callinectes sapidus) were common in warm, shallow, vegetated inland sites during summer-fall. Estuarine-dependent species were common at deeper, more saline locations near the bay during this period. During summer, freshwater species, such as gizzard shad ( Dorosoma cepedianum) and gars ( Lepisosteus spp.), were positively associated with water depth and proximity to the bay. The distribution and abundance of fishes in MIM appear to result from the combined effects of endogenous, seasonal patterns of reproduction and migration operating on large

  5. Mad scientists, compassionate healers, and greedy egotists: the portrayal of physicians in the movies.

    Flores, Glenn

    2002-07-01

    Cinematic depictions of physicians potentially can affect public expectations and the patient-physician relationship, but little attention has been devoted to portrayals of physicians in movies. The objective of the study was the analysis of cinematic depictions of physicians to determine common demographic attributes of movie physicians, major themes, and whether portrayals have changed over time. All movies released on videotape with physicians as main characters and readily available to the public were viewed in their entirety. Data were collected on physician characteristics, diagnoses, and medical accuracy, and dialogue concerning physicians was transcribed. The results showed that in the 131 films, movie physicians were significantly more likely to be male (p film physicians has declined steadily in recent decades. Movie physicians are most commonly surgeons (33%), psychiatrists (26%), and family practitioners (18%). Physicians were portrayed negatively in 44% of movies, and since the 1960s positive portrayals declined while negative portrayals increased. Physicians frequently are depicted as greedy, egotistical, uncaring, and unethical, especially in recent films. Medical inaccuracies occurred in 27% of films. Compassion and idealism were common in early physician movies but are increasingly scarce in recent decades. A recurrent theme is the "mad scientist," the physician-researcher that values research more than patients' welfare. Portrayals of physicians as egotistical and materialistic have increased, whereas sexism and racism have waned. Movies from the past two decades have explored critical issues surrounding medical ethics and managed care. We conclude that negative cinematic portrayals of physicians are on the rise, which may adversely affect patient expectations and the patient-physician relationship. Nevertheless, films about physicians can serve as useful gauges of public opinion about the medical profession, as tools for medical education, and as

  6. Reverse shock index multiplied by Glasgow Coma Scale score (rSIG) is a simple measure with high discriminant ability for mortality risk in trauma patients: an analysis of the Japan Trauma Data Bank.

    Kimura, Akio; Tanaka, Noriko

    2018-04-11

    The shock index (SI), defined as heart rate (HR) divided by systolic blood pressure (SBP), is reported to be a more sensitive marker of shock than traditional vital signs alone. In previous literature, use of the reverse shock index (rSI), taken as SBP divided by HR, is recommended instead of SI for hospital triage. Among traumatized patients aged > 55 years, SI multiplied by age (SIA) might provide better prediction of early post-injury mortality. Separately, the Glasgow Coma Scale (GCS) score has been shown to be a very strong predictor. When considering these points together, rSI multiplied by GCS score (rSIG) or rSIG divided by age (rSIG/A) could provide even better prediction of in-hospital mortality. This retrospective, multicenter study used data from 168,517 patients registered in the Japan Trauma Data Bank for the period 2006-2015. We calculated areas under receiver operating characteristic curves (AUROCs) to measure the discriminant ability by comparing those of SI (or rSI), SIA, rSIG, and rSIG/A for in-hospital mortality and for 24-h blood transfusion. The highest ROC AUC (AUROC), 0.901(0.894-0.908) for in-hospital mortality in younger patients (aged < 55 years), was seen for rSIG. In older patients (aged ≥ 55 years), the AUROC of rSIG/A, 0.845(0.840-0.850), was highest for in-hospital mortality. However, the difference between rSIG and rSIG/A was slight and did not seem to be clinically important. rSIG also had the highest AUROC of 0.745 (0.741-749) for 24-h blood transfusion. rSIG ((SBP/HR) × GCS score) is easy to calculate without the need for additional information, charts or equipment, and can be a more reliable triage tool for identifying risk levels in trauma patients.

  7. Role Identification and Application of SigD in the Transformation of Soybean Phytosterol to 9α-Hydroxy-4-androstene-3,17-dione in Mycobacterium neoaurum.

    Xiong, Liang-Bin; Liu, Hao-Hao; Xu, Li-Qin; Wei, Dong-Zhi; Wang, Feng-Qing

    2017-01-25

    9α-Hydroxy-4-androstene-3,17-dione (9-OHAD) is a valuable steroid pharmaceutical intermediate which can be produced by the conversion of soybean phytosterols in mycobacteria. However, the unsatisfactory productivity and conversion efficiency of engineered mycobacterial strains hinder their industrial applications. Here, a sigma factor D (sigD) was investigated due to its dramatic downregulation during the conversion of phytosterols to 9-OHAD. It was determined as a negative regulator in the metabolism of phytosterols, and the deletion of sigD in a 9-OHAD-producing strain significantly enhanced the titer of 9-OHAD by 18.9%. Furthermore, a high yielding strain was constructed by the combined modifications of sigD and choM2, a key gene in the phytosterol metabolism pathway. After the modifications, the productivity of 9-OHAD reached 0.071 g/L/h (10.27 g/L from 20 g/L phytosterol), which was 22.5% higher than the original productivity of 0.058 g/L/h (8.37 g/L from 20 g/L phytosterol) in the industrial resting cell biotransformation system.

  8. Implementación de un Sistema de Información Geográfica, SIG, en el Parque Nacional Natural Farallones de Cali

    María Victoria Zapata Pardo

    1999-05-01

    Full Text Available El objetivo de este proyecto fue generar una herramienta para mejorar el manejo, conservación y administración del Parque Nacional Natural Farallones de Cali adscrito a la Unidad Administrativa Especial del Sistema de Parques Nacionales Naturales, UAESPNN, dependiente del Ministerio del Medio Ambiente. Con este propósito se implementó un sistema de información geográfica, SIG, como modelo metodológico. El SIG Farallones de CaIi utilizó una base de datos relacional, desarroUada con el software ACCESTM, compatible con los SIG utilizados ARC/INFOTM y ARCI/VIEWTM (para estación de trabajo. Los datos espaciales accesados a la base de datos fueron los de topografía, hidrología, zonas de vida Holdridge, geología, limite, frentes, zonificación con fmes de manejo, precipitación, ocupación indígena, veredas y corregimientos; los cuales contaron con información alfanumérica relacionada, que abarcaba el manejo administrativo, socioeconómíco y tísico entre otros.

  9. Consensus definition of sarcopenia, cachexia and pre-cachexia: joint document elaborated by Special Interest Groups (SIG) "cachexia-anorexia in chronic wasting diseases" and "nutrition in geriatrics".

    Muscaritoli, M; Anker, S D; Argilés, J; Aversa, Z; Bauer, J M; Biolo, G; Boirie, Y; Bosaeus, I; Cederholm, T; Costelli, P; Fearon, K C; Laviano, A; Maggio, M; Rossi Fanelli, F; Schneider, S M; Schols, A; Sieber, C C

    2010-04-01

    Chronic diseases as well as aging are frequently associated with deterioration of nutritional status, loss muscle mass and function (i.e. sarcopenia), impaired quality of life and increased risk for morbidity and mortality. Although simple and effective tools for the accurate screening, diagnosis and treatment of malnutrition have been developed during the recent years, its prevalence still remains disappointingly high and its impact on morbidity, mortality and quality of life clinically significant. Based on these premises, the Special Interest Group (SIG) on cachexia-anorexia in chronic wasting diseases was created within ESPEN with the aim of developing and spreading the knowledge on the basic and clinical aspects of cachexia and anorexia as well as of increasing the awareness of cachexia among health professionals and care givers. The definition, the assessment and the staging of cachexia, were identified as a priority by the SIG. This consensus paper reports the definition of cachexia, pre-cachexia and sarcopenia as well as the criteria for the differentiation between cachexia and other conditions associated with sarcopenia, which have been developed in cooperation with the ESPEN SIG on nutrition in geriatrics. Copyright 2009 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.

  10. Comunicación en sistemas de múltiples robots desde la metodología MAD-Smart

    Jovani Alberto Jiménez Builes

    2008-05-01

    Full Text Available Este artículo demuestra la importancia de la comunicación en sistemas de múltiples robots enmarcado desde la me-todología MAD-Smart. La metodología está compuesta por las fases de conceptuación, análisis y diseño de equipos de múltiples robots mediante la definición de un conjunto de actividades. El propósito de MAD-Smart es ayudar al desarrollador a entender mejor el problema a resolver, las características finales que deberá tener el sistema y el papel que cada agente juega en la solución del problema. La metodología se ha validado mediante la implemen-tación de los proyectos Smart (Sistema multiagente robótico, robótica educativa: máquinas inteligentes en educa-ción y modelo de sensórica y percepción en agentes robóticos para la identificación de materiales. MAD-Smart ha demostrado que es más relevante el manejo de roles, que los agentes mismos para el modelo de comunicación. Lo anterior obedece al grado de abstracción buscado en la metodología, ya que un rol como una representación abs-tracta de un comportamiento de alto nivel permite generalizar de mejor manera los escenarios posibles que se pue-den encontrar en un sistema determinado.

  11. Hubungan Metilasi DNA dengan Ekspresi Gen MADS-box pada Buah Mantel Tanaman Kelapa Sawit (Elaeis guineensis Jacq.

    Maharani Anischan

    2015-03-01

    Full Text Available ABSTRACT The presence of mantled fruit on large-scale clonal production of oil palm had trully decreased the oil productivity. Mantled phenotype is likely to be that of an epigenetic change involving DNA methylation and the MADS-box transcription factor gene which encoded floral organ homeotic transformation. The objectives of this research were to quantify the degree of methylation which determined fruit abnormality through Ultra Performance Liquid Chromatography (UPLC and to study its correlation with the MADS-box EgAGL6, EgAG2, and EgAGA genes expressed on mantled fruit derived from oil palm clonal plants which have been quantified using Quantitative Real-Time PCR (qPCR. This research was arranged in two replications for each gene and cDNA. The expression of the target genes were compared to EF1-α1 as the reference gene. Through the Least Significant Difference (LSD Test at 95% confidence level of qPCR result, EgAGL6 expression was significantly lower in mantled fruit which decreased from 1.88 fold in Abn m to 0.46 fold in Abn. EgAG2 expression was increased non-significantly from 0.91 fold in Abn m to 1.13 fold in Abn, while EgAGA expression was higher in mantled which increased significantly from 1.48 fold in Abn m to 1.71 fold  in Abn. Nuclease S1 digestion and UPLC revealed the genome-wide increase in DNA methylation on mantled fruit (18.33-19.55% compared to its normal counterparts (5.67%. This increased in global DNA methylation was showed by the significant decreased in EgAGL6 transcript level of mantled fruit. This gene assumed to be involved in the development of mantled fruit. Keywords: DNA-methylation, MADS-box genes, Mantled fruit, Quantitative Real-Time PCR

  12. Effect on oxidative stress, hepatic chemical metabolizing parameters, and genotoxic damage of mad honey intake in rats.

    Eraslan, G; Kanbur, M; Karabacak, M; Arslan, K; Siliğ, Y; Soyer Sarica, Z; Tekeli, M Y; Taş, A

    2017-01-01

    A total of 66 male Wistar rats were used and six groups (control: 10 animals and experimental: 12 animals) were formed. While a separate control group was established for each study period, mad honey application to the animals in the experimental group was carried out with a single dose (12.5 g kg -1 body weight (b.w.); acute stage), at a dose of 7.5 g kg -1 b.w. for 21 days (subacute stage), and at a dose of 5 g kg -1 b.w. for 60 days (chronic stage). Tissue and blood oxidative stress markers (malondialdehyde (MDA), nitric oxide (NO), 4-hydroxynonenal (HNE), superoxide dismutase, catalase, glutathione (GSH) peroxidase, and glucose-6-phosphate dehydrogenase), hepatic chemical metabolizing parameters in the liver (cytochrome P450 2E1, nicotinamide adenine dinucleotide (NADH)-cytochrome b5 reductase, nicotinamide adenine dinucleotide phosphate (NADPH)-cytochrome c reductase (CYTC), GSH S-transferase (GST), and GSH), and micronucleus and comet test in some samples were examined. Findings from the study showed that single and repeated doses given over the period increased MDA, NO, and HNE levels while decreasing/increasing tissue and blood antioxidant enzyme activities. From hepatic chemical metabolizing parameters, GST activity increased in the subacute and chronic stages and CYTC activity increased in the acute period, whereas GSH level decreased in the subacute stage. Changes in tail and head intensities were found in most of the comet results. Mad honey caused oxidative stresses for each exposure period and made some significant changes on the comet test in certain periods for some samples obtained. In other words, according to the available research results obtained, careless consumption of mad honey for different medical purposes is not appropriate.

  13. A MADS box protein interacts with a mating-type protein and is required for fruiting body development in the homothallic ascomycete Sordaria macrospora.

    Nolting, Nicole; Pöggeler, Stefanie

    2006-07-01

    MADS box transcription factors control diverse developmental processes in plants, metazoans, and fungi. To analyze the involvement of MADS box proteins in fruiting body development of filamentous ascomycetes, we isolated the mcm1 gene from the homothallic ascomycete Sordaria macrospora, which encodes a putative homologue of the Saccharomyces cerevisiae MADS box protein Mcm1p. Deletion of the S. macrospora mcm1 gene resulted in reduced biomass, increased hyphal branching, and reduced hyphal compartment length during vegetative growth. Furthermore, the S. macrospora Deltamcm1 strain was unable to produce fruiting bodies or ascospores during sexual development. A yeast two-hybrid analysis in conjugation with in vitro analyses demonstrated that the S. macrospora MCM1 protein can interact with the putative transcription factor SMTA-1, encoded by the S. macrospora mating-type locus. These results suggest that the S. macrospora MCM1 protein is involved in the transcriptional regulation of mating-type-specific genes as well as in fruiting body development.

  14. Buying Blood Diamonds and Altering Global Capitalism. Mads Brügger as Unruly Artivist in The Ambassador

    Reestorff, Camilla Møhring

    2013-01-01

    challenges global inequality in relation to finance and mobility. This critique of global inequality is staged through a peculiar ‘‘unruly artivist’’ provocation. Mads Brügger fictionalises his character and over-identifies with the corrupt diplomat seeking to buy and trade blood diamonds. The film is unruly...... because it rejects any explicit ethical claims and norms of participation, thus reproducing the self-same patterns of inequality that it seeks to document. This article studies the film as an unruly documentary that applies satire, cartoon aesthetics, and culture jamming as its artivist strategy...

  15. PRELIMINARY METHODOLOGICAL PROPOSAL FOR ESTIMATING ENVIRONMENTAL FLOWS IN PROJECTS APPROVED BY THE MINISTRY OF ENVIRONMENT AND SUSTAINABLE DEVELOPMENT (MADS, COLOMBIA

    Gabriel A. Pinilla Agudelo

    2014-01-01

    Full Text Available ABSTRACT A methodological proposal for estimating environmental flows in large projects approved by Agencia Nacional de Licencias Ambientales (ANLA in Colombian rivers was developed. The project is the result of an agreement between the MADS and the Universidad Nacional de Colombia, Bogotá (UNC. The proposed method begins with an evaluation of hydrological criteria,continues with a hydraulic and water quality validation, and follows with the determination of habitat integrity. This is an iterative process that compares conditions before and after the project construction and allows to obtain the magnitude of a monthly flow that, besides preserving the ecological functions of the river, guarantees the water uses downstream. Regarding to the biotic component, the proposal includes the establishment and monitoring of biotic integrity indices for four aquatic communities (periphyton, macroinvertebrates, riparian vegetation, and fish. The effects that flow reduction may produce in the medium and long term can be assessed by these indices. We present the results of applying the methodology to several projects licensed by the MADS. RESUMEN Se presenta una propuesta metodológica para estimar los caudales ambientales en grandes proyectos licenciados por la Agencia Nacional de Licencias Ambientales (ANLA de Colombia, resultado de un convenio interadministrativo suscrito entre el ahora Ministerio de Ambiente y Desarrollo Sostenible (MADS de Colombia y la Universidad Nacional de Colombia, Bogotá (UNC. El método propuesto parte de garantizar criterios hidrológicos, continúa con una validación hidráulica y de calidad del agua, sigue con la determinación de la integridad del hábitat, en un proceso iterativo que requiere evaluación para las condiciones antes y después de la construcción del proyecto y que permite establecer un caudal que, además de conservar las funciones ecológicas del río, garantiza los usos del recurso aguas abajo. Espec

  16. The influence of media communication on risk perception and behavior related to mad cow disease in South Korea.

    Park, Jee-Eun; Sohn, Aeree

    2013-08-01

    The purpose of this study was to ascertain the influence of media communication on risk behavior related to mad cow disease (MCD). Mothers of elementary school students in Seoul were recruited as the survey participants of this study. Media reports affected risk behavior related to MCD. Also, knowledge and attitude toward MCD affects risk behavior. Risk-related information provided by the media should maintain consistency and objectivity. For effective risk communication, there should be an open communication between the government and public, experts, and related industries, who should all collaborate.

  17. Assessing Community Coalition Capacity and its Association with Underage Drinking Prevention Effectiveness in the Context of the SPF SIG.

    Flewelling, Robert L; Hanley, Sean M

    2016-10-01

    Community coalitions are a prominent organizational structure through which community-based substance abuse prevention efforts are implemented. There is little empirical evidence, however, regarding the association between coalition attributes and success in achieving community-level reductions in substance abuse behaviors. In this study, we assessed the relationship between coalition capacity, based on coalition coordinator responses to 16 survey items, and reductions in underage drinking prevalence rates. The coalitions were funded through the federally sponsored Strategic Prevention Framework State Incentive Grant (SPF SIG). We first examined whether coalition capacity increased over the life of the projects. Mean capacity scores increased for all 16 capacity items examined (N = 318 coalitions), the majority of which were statistically significant. Analysis of the associations between capacity and reductions in underage drinking was limited to coalitions that targeted underage drinking and provided usable outcome measures based on student survey data for either past 30-day alcohol use (N = 129) or binge drinking (N = 100). Bivariate associations between the capacity items and prevalence reductions for each outcome were consistently positive, although many were not statistically significant. Composite measures of correlated items were then created to represent six different capacity constructs, and included in multivariate models to predict reductions in the targeted outcomes. Constructs that significantly predicted reductions in one or both outcome measures included internal organization and structure, community connections and outreach, and funding from multiple sources. The findings provide support for the expectation that high functioning community coalitions can be effective agents for producing desirable community-level changes in targeted substance abuse behaviors.

  18. USO DE SOFTWARES LIVRES DE SIG COMO UMA FERRAMENTA NO ENSINO DE GEOGRAFIA: MAPEAMENTO DE ÁREAS DE RISCO

    Filipe Silveira Trindade

    2014-09-01

    Full Text Available Esta pesquisa realizou um estudo sobre a viabilidade da utilização de um software livre de Sistemas de Informações Geográficas (SIG pelo professor de Geografia. Mapas temáticos podem ser criados a partir de tais programas e facilitar o ensino em sala. Foram levantados dados para a construção de um mapa temático sobre deslizamento de terra, que fez parte de um dos projetos do Programa Institucional de Bolsa de Iniciação à Docência (PIBID de Geografia na Escola Municipal Ministro Edmundo Lins, em Viçosa-MG. O programa escolhido foi o “TerraView”, criado pelo Instituto Nacional de Pesquisas Espaciais (INPE. Esse software foi estudado para a aquisição de informações sobre o mesmo e posterior manipulação do programa. O mapa temático apresentado aos educandos na sala despertou a atenção dos alunos, principalmente pelo espaço representado (parte da cidade de Viçosa-MG fazer parte da vivência cotidiana do aluno e se mostrar como uma novidade diante deles. Porém, ficou evidente que o software apresenta problemas que devem ser sanados e não é tão simples de ser manuseado, como aponta alguns trabalhos. As tecnologias não substituem o professor, mas pode auxiliá-lo em aula. Assim, é necessário que esses educadores sejam capacitados e qualificados para manipular tais programas.

  19. Revisors opklaring af besvigelser

    Bloch Christensen, Maria; Kristensen, Rikke Holmslykke; Warming-Rasmussen, Bent

    ansvarsfordelingen. Er det bestyrelsen, direktionen, controllerne eller navnlig den eksterne revisor, som burde have opdaget besvigelsen? Eller burde selskabets medarbejdere have påtaget sig det sociale ansvar at agere som whistleblowers? De berørte parter undskylder sig ofte ved, at den nu afslørede bedrager...

  20. Arabidopsis MADS-Box Transcription Factor AGL21 Acts as Environmental Surveillance of Seed Germination by Regulating ABI5 Expression.

    Yu, Lin-Hui; Wu, Jie; Zhang, Zi-Sheng; Miao, Zi-Qing; Zhao, Ping-Xia; Wang, Zhen; Xiang, Cheng-Bin

    2017-06-05

    Seed germination is a crucial checkpoint for plant survival under unfavorable environmental conditions. Abscisic acid (ABA) signaling plays a vital role in integrating environmental information to regulate seed germination. It has been well known that MCM1/AGAMOUS/DEFICIENS/SRF (MADS)-box transcription factors are key regulators of seed and flower development in Arabidopsis. However, little is known about their functions in seed germination. Here we report that MADS-box transcription factor AGL21 is a negative regulator of seed germination and post-germination growth by controlling the expression of ABA-INSENSITIVE 5 (ABI5) in Arabidopsis. The AGL21-overexpressing plants were hypersensitive to ABA, salt, and osmotic stresses during seed germination and early post-germination growth, whereas agl21 mutants were less sensitive. We found that AGL21 positively regulated ABI5 expression in seeds. Consistently, genetic analyses showed that AGL21 is epistatic to ABI5 in controlling seed germination. Chromatin immunoprecipitation assays further demonstrated that AGL21 could directly bind to the ABI5 promoter in plant cells. Moreover, we found that AGL21 responded to multiple environmental stresses and plant hormones during seed germination. Taken together, our results suggest that AGL21 acts as a surveillance integrator that incorporates environmental cues and endogenous hormonal signals into ABA signaling to regulate seed germination and early post-germination growth. Copyright © 2017 The Author. Published by Elsevier Inc. All rights reserved.

  1. The MADS-box transcription factor FgMcm1 regulates cell identity and fungal development in Fusarium graminearum.

    Yang, Cui; Liu, Huiquan; Li, Guotian; Liu, Meigang; Yun, Yingzi; Wang, Chenfang; Ma, Zhonghua; Xu, Jin-Rong

    2015-08-01

    In eukaryotic cells, MADS-box genes are known to play major regulatory roles in various biological processes by combinatorial interactions with other transcription factors. In this study, we functionally characterized the FgMCM1 MADS-box gene in Fusarium graminearum, the causal agent of wheat and barley head blight. Deletion of FgMCM1 resulted in the loss of perithecium production and phialide formation. The Fgmcm1 mutant was significantly reduced in virulence, deoxynivalenol biosynthesis and conidiation. In yeast two-hybrid assays, FgMcm1 interacted with Mat1-1-1 and Fst12, two transcription factors important for sexual reproduction. Whereas Fgmcm1 mutants were unstable and produced stunted subcultures, Fgmcm1 mat1-1-1 but not Fgmcm1 fst12 double mutants were stable. Furthermore, spontaneous suppressor mutations occurred frequently in stunted subcultures to recover growth rate. Ribonucleic acid sequencing analysis indicated that a number of sexual reproduction-related genes were upregulated in stunted subcultures compared with the Fgmcm1 mutant, which was downregulated in the expression of genes involved in pathogenesis, secondary metabolism and conidiation. We also showed that culture instability was not observed in the Fvmcm1 mutants of the heterothallic Fusarium verticillioides. Overall, our data indicate that FgMcm1 plays a critical role in the regulation of cell identity, sexual and asexual reproduction, secondary metabolism and pathogenesis in F. graminearum. © 2015 Society for Applied Microbiology and John Wiley & Sons Ltd.

  2. THE DISTANT STRATUM OF UNREASON: EXPRESSIONS OF MADNESS IN THE PORT AND CITY OF MAZATLÁN LATE NINETEENTH CENTURY

    Griselda Santiago-Pérez

    2017-07-01

    Full Text Available This work deals with aspects of life port, with respect to the inconveniences and constant challenges on the different epidemics and diseases that the inhabitants faced, as well as the role played by the hospital institutions. Aspects taken into account to envision the other side of reality, in opposition to the modernity and progress alluded to by the ruling elite during the Porfirian years, who tried to disguise precariousness in the face of the insolvency of health care. In addition to this, he pays attention mainly to the madness manifestations of demented men and women inside the city and port of Mazatlán in the last years of the XIX century, specifically between 1877 and 1900. The presence of these marked an important guideline between the city for the sake of progress and the ephemeral reality that they faced in relation to the health and order that was intended to have. And in addition it allows to observe the perspective that was had of them, as well as the construction and the association of the causes on the madness. With the participation of these maladjusted individuals, transgressors of order, social deviance and poverty, who tried to be evaded, limiting them to the integration of social coexistence. Under the establishment of disciplinary forms, governed by rules, customs and routines that guided his conduct. The segregation by the mode of behavior, determined the construction of stereotypes by society, the family, doctors and public authorities.

  3. Preliminary methodological proposal for estimating environmental flows in projects approved by the ministry of environment and sustainable development (MADS), Colombia

    Pinilla Agudelo, Gabriel A; Rodriguez Sandoval, Erasmo A; Camacho Botero, Luis A

    2014-01-01

    A methodological proposal for estimating environmental flows in large projects approved by Agencia Nacional de Licencias Ambientales (ANLA) in Colombian rivers was developed. The project is the result of an agreement between the MADS and the Universidad Nacional de Colombia, Bogota (UNC). The proposed method begins with an evaluation of hydrological criteria, continues with a hydraulic and water quality validation, and follows with the determination of habitat integrity. This is an iterative process that compares conditions before and after the project construction and allows to obtain the magnitude of a monthly flow that, besides preserving the ecological functions of the river, guarantees the water uses downstream. Regarding to the biotic component, the proposal includes the establishment and monitoring of biotic integrity indices for four aquatic communities (periphyton, macro invertebrates, riparian vegetation, and fish). The effects that flow reduction may produce in the medium and long term can be assessed by these indices. We present the results of applying the methodology to several projects licensed by the MADS.

  4. Concurso de Universidades - SB10mad “Ciudad sostenible: la rehabilitación como herramienta”

    González Díaz, M. J.

    2011-10-01

    Full Text Available The competition was suggested by GBCEspaña and developed by ASA (Sustainability and Architecture Association, according to the items of SB10Mad Conference. The aim was to involve Spain and other Universities abroad in the study of revitalizing existing areas. Contestants had to suggest solutions to reach benefits in a neighborhood selected by themselves, in subjects such as resource’s economy, energy, water, social improvements and mitigating pollution. The success on variety and the quality of solutions suggest this matter as good university training and successful in developing ideas.

    El concurso fue una propuesta de GBCEspaña desarrollada por ASA (Asociación Sostenibilidad y Arquitectura, en concordancia con el Congreso SB10mad, con el objetivo de involucrar a las Universidades (españolas y extranjeras en las estrategias de revitalización de barrios existentes. Los concursantes debían proponer soluciones en materia de economía de recursos, energía, ciclo del agua, mejoras sociales y reducción de contaminación, sobre un barrio de su propia elección. El éxito de participación y calidad en las propuestas permite aventurar esta materia como de gran interés en la formación universitaria y en el desarrollo de soluciones.

  5. Overexpression of the class D MADS-box gene Sl-AGL11 impacts fleshy tissue differentiation and structure in tomato fruits

    MADS-box transcription factors are key elements of the genetic networks controlling flower and fruit development. Among these, the class D clade are involved in seed, ovule, and funiculus development. The tomato genome comprises two class D genes, Sl-AGL11 and Sl-MBP3, both displaying high expressio...

  6. Banana MaMADS transcription factors are necessary for fruit ripening and molecular tools to promote shelf-life and food security

    Genetic solutions to postharvest crop loss can reduce cost and energy inputs while increasing food security, especially for banana (Musa acuminata), which is a significant component of worldwide food commerce. We have functionally characterized two banana E class (SEPALLATA3 [SEP3]) MADS box genes, ...

  7. 'Where of is mad al mankynde' : an edition of and introduction to the twenty-four poems in Oxford, Bodleian Library, MS Digby 102

    Verheij, Louis Johan Philip

    2009-01-01

    'Where of is Mad al Mankynde' represents a new critical edition of the collection of twenty-four late-medieval anonymous poems contained, among other pieces, in Oxford, Bodleian Library, MS Digby 102. Each poem is introduced with a brief summary and closes with line-for-line explanatory comments.

  8. Identification and quantification of expression levels of three FRUITFULL-like MADS-box genes from the orchid Dendrobium thyrsiflorum (Reichb. f.)

    Skipper, Martin; Pedersen, Kim B.; Johansen, Louise Buchholt

    2005-01-01

    Orchids serve as useful model plants for the discovery and study of genes involved in novel processes in floral development because of their highly modified flowers. In this study three different FRUITFULL (FUL)-like MADS-box genes, DthyrFL1, -2, and -3 have been isolated from the orchid Dendrobium...

  9. Construire ses cartes : le développement d’outils statistiques interactifs intégrés à un SIG

    Benoît Ogier

    1999-06-01

    Full Text Available Les systèmes d’exploitation des micro-ordinateurs proposent aujourd’hui des interfaces conviviales. En outre, certains micro-SIG offrent des possibilités intéressantes de développement par l’intermédiaire de " plug ins ", dans les langages de programmation les plus courants (Pascal, C, Delphi.... C’est sur ces bases que nous avons pu compléter les fonctionnalités d’un micro-SIG (requêtes spatiales, calcul d’itinéraire, gestion de données, cartographie..., par une série de modules statistiques et graphiques.Ces outils mettent à la disposition de l’utilisateur, toutes les informations statistiques et représentations, nécessaires à une analyse complète des données. En effet, les modules sont liés entre eux, ce qui permet d’aborder une étude sous plusieurs angles simultanément, et de comparer les résultats de différentes méthodes. De plus, une mise a jour automatique de la carte s’effectue dès que l’on procède à une mise en classe à l’aide d’un des modules. On est donc en présence d’une complète interactivité SIG-Cartes-Statistiques.Mais on peut aller plus loin et envisager l’expérimentation d’autres outils graphiques, notamment pour l’exploitation des résultats d’analyses factorielles.

  10. Forebyggelse betaler sig

    Bencke, Jesper

    2006-01-01

    Artilen gennemgår hvilke biomekaniske mekanismer, som kan influere på risikoen for en korsbåndsskade i håndbold. Desuden diskuteres hvorledes forebyggelse kan reducere riskikoen for korsbåndsskade. Udgivelsesdato: December 2006...

  11. Folkekirkeskizofrenien breder sig

    Lægaard, Sune

    2013-01-01

    Et nyligt eksempel på fænomenet kan læses i en kronik i Kristeligt Dagblad, hvor to folkekirkepræster både mistænkeliggør kirkeministeren og det udvalg, han har nedsat, mener lektor, ph.d. Sune Lægaard...

  12. SigWx-Low

    National Oceanic and Atmospheric Administration, Department of Commerce — The low-level graphics product is a forecast of aviation weather hazards, primarily intended to be used as a guidance product for briefing the VFR pilot. The...

  13. Herramientas SIG 3D

    Francisco R. Feito Higueruela

    2010-04-01

    Full Text Available Applications of Geographical Information Systems on several Archeology fields have been increasing during the last years. Recent avances in these technologies make possible to work with more realistic 3D models. In this paper we introduce a new paradigm for this system, the GIS Thetrahedron, in which we define the fundamental elements of GIS, in order to provide a better understanding of their capabilities. At the same time the basic 3D characteristics of some comercial and open source software are described, as well as the application to some samples on archeological researchs

  14. Genome-wide analysis of the MADS-box gene family in polyploid cotton (Gossypium hirsutum) and in its diploid parental species (Gossypium arboreum and Gossypium raimondii).

    Nardeli, Sarah Muniz; Artico, Sinara; Aoyagi, Gustavo Mitsunori; de Moura, Stéfanie Menezes; da Franca Silva, Tatiane; Grossi-de-Sa, Maria Fatima; Romanel, Elisson; Alves-Ferreira, Marcio

    2018-06-01

    The MADS-box gene family encodes transcription factors that share a highly conserved domain known to bind to DNA. Members of this family control various processes of development in plants, from root formation to fruit ripening. In this work, a survey of diploid (Gossypium raimondii and Gossypium arboreum) and tetraploid (Gossypium hirsutum) cotton genomes found a total of 147, 133 and 207 MADS-box genes, respectively, distributed in the MIKC, Mα, Mβ, Mγ, and Mδ subclades. A comparative phylogenetic analysis among cotton species, Arabidopsis, poplar and grapevine MADS-box homologous genes allowed us to evaluate the evolution of each MADS-box lineage in cotton plants and identify sequences within well-established subfamilies. Chromosomal localization and phylogenetic analysis revealed that G. raimondii and G. arboreum showed a conserved evolution of the MIKC subclade and a distinct pattern of duplication events in the Mα, Mγ and Mδ subclades. Additionally, G. hirsutum showed a combination of its parental subgenomes followed by a distinct evolutionary history including gene gain and loss in each subclade. qPCR analysis revealed the expression patterns of putative homologs in the AP1, AP3, AGL6, SEP4, AGL15, AG, AGL17, TM8, SVP, SOC and TT16 subfamilies of G. hirsutum. The identification of putative cotton orthologs is discussed in the light of evolution and gene expression data from other plants. This analysis of the MADS-box genes in Gossypium species opens an avenue to understanding the origin and evolution of each gene subfamily within diploid and polyploid species and paves the way for functional studies in cotton species. Copyright © 2018 Elsevier Masson SAS. All rights reserved.

  15. Desarrollo de una base de datos integrada de Censo y encuesta mediante el uso de elementos de inteligencia de negocios y SIG

    Cornejo,Robert; Navarrete,Mónica; Valdivia,Ricardo; Aroca,Patricio; Aracena,Sebastián

    2014-01-01

    En este trabajo se plantea el desarrollo de una solución de Inteligencia de Negocios y Sistemas de Información Geográfica (SIG) para gestionar integradamente los datos generados por el Censo Nacional de personas, hogares y viviendas y la Encuesta de Clasificación Económica y Social (CASEN) como una alternativa a los métodos actuales de estimación en área pequeña (SAE) utilizados para obtener indicadores desagregados de bienestar y que estiman, por ejemplo, el ingreso a partir de los atributos...

  16. Uso de las imágenes de satélites y los SIG en el campo de la Ingeniería Agrícola

    Dámaso Ponvert Delisles; Andés Lau Quan

    2013-01-01

    Los sistemas de ingeniería agrícola constituyen conjuntos de técnicas y tecnologías que garantizan la producción agropecuaria. Por lo general, ellos producen determinados impactos al medio ambiente, que tienen consecuencias negativas sobre los recursos naturales produciendo huellas en el territorio. En la actualidad se aplican con éxito tecnologías muy eficaces como la Teledetección y, los SIG para identificar la huella espacial de estos impactos y apoyar las decisiones que ayuden a los direc...

  17. Análisis SIG Libre aplicado a Cooperación al Desarrollo. Reflexiones sobre datos de los agentes de cooperación en Galicia

    Varela García, Francisco Alberto; Carballido Sanjurio, Roi; Puga Alonso, Francisco; Molejón Quintana, Carmen; Martínez Crespo, Gonzalo

    2011-01-01

    Presentación de una reflexión particular sobre la utilidad de emplear el SIG libre para trabajar en Cooperación al Desarrollo y para analizar las inversiones de la Administración Pública en este ámbito. Los planteamientos expuestos están basados en trabajos y experiencias vividas desde el Cartolab, tanto en participaciones en proyectos de cooperación, como en trabajos de análisis del uso de las tecnologías de la información geográfica en agentes de cooperación y un estudio sobre la inversión ...

  18. Integrando SIG e USLE para mapeamento da perda de solo em área de proteção ambiental

    Fernando Jorge Corrêa Magalhães Filho; Fabio Martins Ayres; Teodorico Alves Sobrinho

    2014-01-01

    O uso de geotecnologias no planejamento ambiental tem sido instrumento amplamente utilizado. Nesse estudo utilizou-se de um Sistema de Informações Geográficas (SIG) e da Linguagem Espacial para Geoprocessamento Algébrico (LEGAL) para o processamento da Equação Universal de Perda de Solos (USLE), para avaliação dos processos erosivos em área de proteção ambiental. Embora a USLE tenha o objetivo quantitativo de estimar a perda anual média de solo por erosão hídrica, o trabalho utilizou-se de da...

  19. LOS SISTEMAS DE INFORMACIÓN GEOGRÁFICA (SIG), UNA GRAN HERRAMIENTA PARA LA SILVICULTURA URBANA GEOGRAPHICAL INFORMATION SYSTEMS (GIS), A GREAT TOOL FOR URBAN SYLVICULTURE

    Leodán Andrés Otaya Burbano; Robinson de Jesús Sánhez Zapata; León Morales Soto; Verónica Botero Fernández

    2006-01-01

    Como una fase piloto, para adquirir experiencia, definir metodologías y determinar las ventajas de los Sistemas de Información Geográfica (SIG) al ser aplicados en la Silvicultura Urbana, se realizó el inventario, diagnóstico, propuesta de manejo y valoración económica del Bosque Urbano del barrio La Magnolia del Municipio de Envigado, Departamento de Antioquia, Colombia. Para el manejo y análisis de los registros recolectados en campo, se diseñó una base de datos en el software Microsoft Acc...

  20. Carlos de Sigüenza y Góngora — el paraíso terrenal en la Nueva España // Carlos de Sigüenza y Góngora — The idea of the earthly paradise in New Spain

    Katarína Zatlkajová

    2016-12-01

    Full Text Available This paper deals with different possible ways to approach the idea of the “Earthly Paradise” in a religious chronicle, written by Carlos de Sigüenza y Góngora. His work connects the mythological and utopian understanding of the “Paradise”, placed in the West, with its religious and allegorical dimension. His possible aim is to display the patriotic pride through the hagiographical depiction of virtuous nuns.

  1. Cloning, Structural Characterization, and Phylogenetic Analysis of Flower MADS-Box Genes from Crocus (Crocus sativus L.

    Athanasios S. Tsaftaris

    2007-01-01

    Full Text Available Crocus (Crocus sativus L. is a crop species cultivated for its flowers and, more specifically, for its red stigmas. The flower of crocus is bisexual and sterile, since crocus is a triploid species. Its perianth consists of six petaloid tepals: three tepals in whorl 1 (outer tepals and three tepals in whorl 2 (inner tepals. The androecium consists of three distinct stamens and the gynoecium consists of a single compound pistil with three carpels, a single three-branched style, and an inferior ovary. The dry form of the stigmas constitutes the commercial saffron used as a food additive, in the coloring industry, and in medicine. In order to uncover and understand the molecular mechanisms controlling flower development in cultivated crocus and its relative wild progenitor species, and characterize a number of crocus flower mutants, we have cloned and characterized different, full-length, cDNA sequences encoding MADS-box transcription factor proteins involved in flower formation.

  2. Madness, Monstrosity and Writing: Towards a Genealogical Analysis in 'El obsceno pájaro de la noche'

    Santiago Juan-Navarro

    2016-06-01

    Full Text Available Drawing on Foucault’s concept of “genealogy,” this article explores madness, monstrosity and writing in José Donoso´s The Obscene Bird of Night(1970. Like Foucault, Donoso is interested in studying the body as “the inscribed surface of events,” the locus of a dissociated self (adopting the illusion of a substantial unity, and a mass in perpetual disintegration. Like Donoso, Foucault assaults enlightened reason and the principle of reality in order to subvert and transgress the instrumental rationality and normalcy of bourgeois culture. This essay analyzes Donoso’s novel in light of these concepts in order to open a new path for understanding one of the most obscure texts in modern Latin American fiction.

  3. Conifer reproductive development involves B-type MADS-box genes with distinct and different activities in male organ primordia.

    Sundström, Jens; Engström, Peter

    2002-07-01

    The Norway spruce MADS-box genes DAL11, DAL12 and DAL13 are phylogenetically related to the angiosperm B-function MADS-box genes: genes that act together with A-function genes in specifying petal identity and with C-function genes in specifying stamen identity to floral organs. In this report we present evidence to suggest that the B-gene function in the specification of identity of the pollen-bearing organs has been conserved between conifers and angiosperms. Expression of DAL11 or DAL12 in transgenic Arabidopsis causes phenotypic changes which partly resemble those caused by ectopic expression of the endogenous B-genes. In similar experiments, flowers of Arabidopsis plants expressing DAL13 showed a different homeotic change in that they formed ectopic anthers in whorls one, two or four. We also demonstrate the capacity of the spruce gene products to form homodimers, and that DAL11 and DAL13 may form heterodimers with each other and with the Arabidopsis B-protein AP3, but not with PI, the second B-gene product in Arabidopsis. In situ hybridization experiments show that the conifer B-like genes are expressed specifically in developing pollen cones, but differ in both temporal and spatial distribution patterns. These results suggest that the B-function in conifers is dual and is separated into a meristem identity and an organ identity function, the latter function possibly being independent of an interaction with the C-function. Thus, even though an ancestral B-function may have acted in combination with C to specify micro- and megasporangia, the B-function has evolved differently in conifers and angiosperms.

  4. Dormancy-Associated MADS-Box (DAM) and the Abscisic Acid Pathway Regulate Pear Endodormancy Through a Feedback Mechanism.

    Tuan, Pham Anh; Bai, Songling; Saito, Takanori; Ito, Akiko; Moriguchi, Takaya

    2017-08-01

    In the pear 'Kosui' (Pyrus pyrifolia Nakai), the dormancy-associated MADS-box (PpDAM1 = PpMADS13-1) gene has been reported to play an essential role in bud endodormancy. Here, we found that PpDAM1 up-regulated expression of 9-cis-epoxycarotenoid dioxygenase (PpNCED3), which is a rate-limiting gene for ABA biosynthesis. Transient assays with a dual luciferase reporter system (LUC assay) and electrophoretic mobility shift assay (EMSA) showed that PpDAM1 activated PpNCED3 expression by binding to the CArG motif in the PpNCED3 promoter. PpNCED3 expression was increased toward endodormancy release in lateral flower buds of 'Kosui', which is consistent with the induced levels of ABA, its catabolism (ABA 8'-hydroxylase) and signaling genes (type 2C protein phosphatase genes and SNF1-related protein kinase 2 genes). In addition, we found that an ABA response element (ABRE)-binding transcription factor, PpAREB1, exhibiting high expression concomitant with endodormancy release, bound to three ABRE motifs in the promoter region of PpDAM1 and negatively regulated its activity. Taken together, our results suggested a feedback regulation between PpDAM1 and the ABA metabolism and signaling pathway during endodormancy of pear. This first evidence of an interaction between a DAM and ABA biosynthesis in vitro will provide further insights into bud endodormancy regulatory mechanisms of deciduous trees including pear. © The Author 2017. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com.

  5. Comprehensive Cloning of Prunus mume Dormancy Associated MADS-Box Genes and Their Response in Flower Bud Development and Dormancy

    Kai Zhao

    2018-02-01

    Full Text Available Dormancy Associated MADS-box genes are SVP/MADs-box members and supposed to play crucial roles in plant dormancy of perennial species. In Prunus mume, PmDAM6 has been previously identified to induce plant dormancy. In the current study, six PmDAMs were cloned in P. mume and functionally analyzed in yeast and tobacco to detect the roles of the genes paralogous to PmDAM6. The expression patterns together with sequence similarities indicate that PmDAMs are divided into two sub-clades within SVP group. Moreover, PmDAMs are verified to take part in the development of different plant organs, specifically the flower buds, in some intricate patterns. Furthermore, the PmDAM proteins are found to have special functions by forming corresponding protein complex during the development of flower bud and induction of dormancy. In particular, when PmDAM1 dominating in flower bud in the warm months, the protein complexes are consisted of PmDAM1 itself or with PmDAM2. With the decrease temperatures in the following months, PmDAM6 was found to be highly expressed and gradually changed the complex structure to PmDAM6-protein complex due to strong binding tendencies with PmDAM1 and PmDAM3. Finally, the homodimers of PmDAM6 prevailed to induce the dormancy. The results obtained in the current study highlight the functions of PmDAMs in the tissue development and dormancy, which provide available suggestions for further explorations of protein-complex functions in association with bud growth and dormancy.

  6. Morphological "primary homology" and expression of AG-subfamily MADS-box genes in pines, podocarps, and yews.

    Englund, Marie; Carlsbecker, Annelie; Engström, Peter; Vergara-Silva, Francisco

    2011-01-01

    The morphological variation among reproductive organs of extant gymnosperms is remarkable, especially among conifers. Several hypotheses concerning morphological homology between various conifer reproductive organs have been put forward, in particular in relation to the pine ovuliferous scale. Here, we use the expression patterns of orthologs of the ABC-model MADS-box gene AGAMOUS (AG) for testing morphological homology hypotheses related to organs of the conifer female cone. To this end, we first developed a tailored 3'RACE procedure that allows reliable amplification of partial sequences highly similar to gymnosperm-derived members of the AG-subfamily of MADS-box genes. Expression patterns of two novel conifer AG orthologs cloned with this procedure-namely PodAG and TgAG, obtained from the podocarp Podocarpus reichei and the yew Taxus globosa, respectively-are then further characterized in the morphologically divergent female cones of these species. The expression patterns of PodAG and TgAG are compared with those of DAL2, a previously discovered Picea abies (Pinaceae) AG ortholog. By treating the expression patterns of DAL2, PodAG, and TgAG as character states mapped onto currently accepted cladogram topologies, we suggest that the epimatium-that is, the podocarp female cone organ previously postulated as a "modified" ovuliferous scale-and the canonical Pinaceae ovuliferous scale can be legitimally conceptualized as "primary homologs." Character state mapping for TgAG suggests in turn that the aril of Taxaceae should be considered as a different type of organ. This work demonstrates how the interaction between developmental-genetic data and formal cladistic theory could fruitfully contribute to gymnosperm systematics. © 2011 Wiley Periodicals, Inc.

  7. LA POTENCIALIDAD DEL TERRITORIO EN LA RESTAURACIÓN ECOLÓGICA EL USO DE HERRAMIENTAS SIG PARA ESTABLECER PRIORIDADES DE RESTAURACIÓN ECOLÓGICA

    CORZO RAMÍREZ LILIANA

    2012-12-01

    Full Text Available El presente artículo desarrolla una propuesta de diálogo interdisciplinar a través del uso de herramientas SIG (Sistemas de Información Geográfica para la construcción de una metodología de identificación del Potencial de Restauración Ecológica (PRE, en áreas de borde urbano que hacen parte de la Estructura Ecológica Principal de Bogotá, según el ordenamiento territorial. Estas áreas suscriben problemáticas socio ambientales particulares propias de la interacción entre naturaleza y cultura. En tal sentido, se describen los pasos metodológicos para la identificación del PRE: caracterización biótica, física y social; identificación y espacialización de variables; ponderación y cruces para el cálculo del potencial final. Luego se discute sobre los alcances y limitaciones del uso de herramientas SIG como posibilidad metodológica para la interacción entre disciplinas como la biología, la geografía física y la sociología durante el proceso de restauración ecológica.

  8. Integrando SIG e USLE para mapeamento da perda de solo em área de proteção ambiental

    Fernando Jorge Corrêa Magalhães Filho

    2014-04-01

    Full Text Available O uso de geotecnologias no planejamento ambiental tem sido instrumento amplamente utilizado. Nesse estudo utilizou-se de um Sistema de Informações Geográficas (SIG e da Linguagem Espacial para Geoprocessamento Algébrico (LEGAL para o processamento da Equação Universal de Perda de Solos (USLE, para avaliação dos processos erosivos em área de proteção ambiental. Embora a USLE tenha o objetivo quantitativo de estimar a perda anual média de solo por erosão hídrica, o trabalho utilizou-se de dados para mapeamento das áreas vulneráveis ao processo erosivo. O uso integrado de SIG com a USLE facilita o planejamento e a tomada de decisões na proposição de medidas adequadas no plano de manejo para promover a conservação da água e do solo em unidades de conservação ambiental.

  9. Pemetaan Potensi Kekeringan Lahan se-pulau Batam menggunakan Teknik Sistem Informasi Geografis (SIG dan Penginderaan Jauh

    Titi Aprilliyanti

    2017-04-01

    Full Text Available Kekeringan merupakan hubungan antara ketersediaan air yang jauh dibawah kebutuhan baik untuk kebutuhan hidup, pertanian, kegiatan ekonomi dan lingkungan. Informasi mengenai potensi kekeringan sangat diperlukan untuk pencegahan ataupun penanggulangan untuk mengurangi dampak negatif yang ditimbulkan. Adapun tujuan dari penelitian ini adalah menghasilkan peta potensi kekeringan lahan di Batam yang berbasis web. Dalam penelitian ini memanfaatkan teknik penginderaan jauh dan SIG. Penggunaan citra landsat 8 untuk menentukan nilai LST (Land Surface Temperature dan penggunaan lahan kemudian di overlay dan dilakukan scoring. Tahap akhir penelitian yaitu melakukan validasi terhadap parameter-parameter yang mempengaruhi dengan mengambil beberapa sampel. Adapun hasil akhir dari penelitian ini adalah peta potensi kekeringan se-pulau Batam yang memiliki 5 kelas potensi kekeringan. Kelas potensi kekeringan sangat rendah dengan  luas area 2629.45 ha yang dominan terletak pada Kecamatan Sungai Beduk, Sekupang dan Batu Aji. Kelas potensi kekeringan rendah dengan luas area 9585.521 ha yang dominan terletak pada Kecamatan Sekupang. Kelas potensi kekeringan sedang dengan  luas area 9507.12 ha yang dominan terletak pada Kecamatan Sekupang. Kelas potensi kekeringan tinggi dengan  luas area 7081.392 ha yang dominan terletak pada Kecamatan Sekupang, Sagulung dan Nongsa. Kelas potensi kekeringan sangat tinggi dengan luas area 15600.12 ha yang dominan terletak pada Kecamatan Batam Kota dan Nongsa.   Drought is the relationship between the availability of water is far below the need both for the necessities of life, agriculture, economic activities and the environment. Information about potential droughts is indispensable for the prevention or mitigation to reduce the negative impact caused. As for the purpose of this research is to produce a map of potential drought land in the Batam-based web. In this research utilising remote sensing and GIS techniques. The use of

  10. LAS CUEVAS DE LA LLUERA (SAN JUAN DE PRIORIO, OVIEDO, ASTURIAS, ESPAÑA: ELABORACIÓN MEDIANTE UN SIG DE UNA ESTRATIGRAFÍA SOLUTRENSE

    José Adolfo Rodríguez Asensio

    2013-11-01

    Full Text Available El uso de modelos arqueológicos en 3D cada día tiene más auge en arqueología. En estos últimos años, arqueología y tecnologías de la información se han convertido en un gran binomio. Ahora bien, los modelos arqueológicos en 3D no son simples recreaciones virtuales, sino que siempre deben ajustarse a criterios científicos. Dentro de los modelos arqueológicos en 3D, nosotros nos centramos en el mundo de los Sistemas de Información Geográfica (SIG y en la realización de la estratigrafía solutrense de las cuevas de La Lluera (Asturias, España. Abordamos la elaboración de la estratigrafía solutrense de las mencionadas cuevas como un proceso interactivo de restitución de la realidad arqueológica, un proceso de validación de los datos y un proceso de simulación. Gracias al mencionado proceso, cuyo resultado es la realización mediante un SIG de una estratigrafía solutrense, constatamos qué los SIG son la herramienta adecuada para abordar interdisciplinarmente el estudio de las cuevas de La Lluera.The scientific use of 3D models every day has more heyday in archaeology. Archaeology and information technologies have turned in the latter years into a big binomial. Now then, the archaeological models in 3D are not simple virtual recreations, but they must always fit to scientific criteria. Inside the world of the archaeological models in 3D, we center in the field of the GIS or Geographical information Systems and in the achievement of the solutrean stratigraphy of La Lluera caves (Asturias, Spain. We raise the making of the solutrean stratigraphy of the above mentioned cave like an interactive process of restitution of the archaeological reality, data validation and simulation. Thanks to the above mentioned process, whose outcome is the realization by GIS a solutrean stratigraphy, see what GIS are the appropriate tool to address interdisciplinarmente the study of the La Lluera caves.

  11. Análise de decisão multicritério na localização de usinas termoelétricas utilizando SIG

    Kátia Lívia Zambon

    2005-08-01

    Full Text Available A integração de sistemas de informação geográfica (SIGs e de métodos de decisão multicritério vêm proporcionando inúmeros benefícios para a resolução de problemas de planejamento e gerenciamento do mundo real. Sob esta ótica, este trabalho se propõe a avaliar as alternativas geradas em ambiente SIG para a localização de usinas termoelétricas (UTEs, aplicando técnicas de análise multicritério no processo de decisão. O modelo desenvolvido contribui para a difícil e complexa tarefa de analisar locais com potencial para a instalação de uma UTE e os diversos critérios envolvidos no problema, sejam eles de ordem econômica e/ou ambiental. A visualização dos resultados, através de mapas no SIG, mostra a aplicabilidade das técnicas de análise multicritério para o planejamento da expansão do setor elétrico. Além disso, os resultados, aplicados em um estudo de caso real, mostram cenários com situações de risco mínimo, médio (trade-off total e máximo para uma tecnologia selecionada para a instalação da UTE.The combination of Geographic Information Systems (GIS and Multicriteria Decision Analysis (MCDA methods can bring several benefits for the solution of real-world planning and management problems. Under that view, this work aims to evaluate alternatives for the location of Thermoelectric Power Plants (TPP produced in a GIS environment using a MCDA technique for supporting the decision-making process. The proposed model is a contribution for the TPP site selection process, which is a difficult and complex task due to the several economic and environmental criteria it involves. The visualization of the analysis outcome in GIS maps helps to demonstrate the suitability of MCDA techniques for planning the expansion of the electric supply system. In addition, the selection of a specific technology for the installation of the TPP in the real case application is used to show scenarios with different risk levels, which

  12. Investigating the Impact of a Genome-Wide Supported Bipolar Risk Variant of MAD1L1 on the Human Reward System.

    Trost, Sarah; Diekhof, Esther K; Mohr, Holger; Vieker, Henning; Krämer, Bernd; Wolf, Claudia; Keil, Maria; Dechent, Peter; Binder, Elisabeth B; Gruber, Oliver

    2016-10-01

    Recent genome-wide association studies have identified MAD1L1 (mitotic arrest deficient-like 1) as a susceptibility gene for bipolar disorder and schizophrenia. The minor allele of the single-nucleotide polymorphism (SNP) rs11764590 in MAD1L1 was associated with bipolar disorder. Both diseases, bipolar disorder and schizophrenia, are linked to functional alterations in the reward system. We aimed at investigating possible effects of the MAD1L1 rs11764590 risk allele on reward systems functioning in healthy adults. A large homogenous sample of 224 young (aged 18-31 years) participants was genotyped and underwent functional magnetic resonance imaging (fMRI). All participants performed the 'Desire-Reason Dilemma' paradigm investigating the neural correlates that underlie reward processing and active reward dismissal in favor of a long-term goal. We found significant hypoactivations of the ventral tegmental area (VTA), the bilateral striatum and bilateral frontal and parietal cortices in response to conditioned reward stimuli in the risk allele carriers compared with major allele carriers. In the dilemma situation, functional connectivity between prefrontal brain regions and the ventral striatum was significantly diminished in the risk allele carriers. Healthy risk allele carriers showed a significant deficit of their bottom-up response to conditioned reward stimuli in the bilateral VTA and striatum. Furthermore, functional connectivity between the ventral striatum and prefrontal areas exerting top-down control on the mesolimbic reward system was reduced in this group. Similar alterations in reward processing and disturbances of prefrontal control mechanisms on mesolimbic brain circuits have also been reported in bipolar disorder and schizophrenia. Together, these findings suggest the existence of an intermediate phenotype associated with MAD1L1.

  13. An integrin alpha4beta7•IgG heterodimeric chimera binds to MAdCAM-1 on high endothelial venules in gut-associated lymphoid tissue.

    Hoshino, Hitomi; Kobayashi, Motohiro; Mitoma, Junya; Sato, Yoshiko; Fukuda, Minoru; Nakayama, Jun

    2011-06-01

    Lymphocyte homing is regulated by a multistep process mediated by sequential adhesive interactions between circulating lymphocytes and high endothelial venules (HEVs). In gut-associated lymphoid tissue (GALT), the initial interactive step, "tethering and rolling," is partly mediated by integrin α4β7 expressed on GALT-homing lymphocytes and its ligand MAdCAM-1, which is exclusively expressed on HEVs in GALT. To probe functional MAdCAM-1 in tissue sections, we developed a soluble integrin α4β7 heterodimeric IgG chimera by joining the extracellular region of mouse integrin α4 and β7 subunits to a human IgG Fc domain. Western blot analysis revealed that co-transfection of HEK 293T cells with expression vectors encoding integrin α4•IgG and β7•IgG results in the formation of α4β7•IgG heterodimeric chimeras. This complex preferentially binds to CHO cells expressing MAdCAM-1 and, to a lesser extent, to cells expressing VCAM-1, but not to cells expressing ICAM-1. Moreover, α4β7•IgG specifically binds to HEVs in GALT in situ in a divalent cation-dependent fashion and inhibits lymphocyte binding to HEVs in GALT. These findings indicate that α4β7•IgG can be used as a probe for functional MAdCAM-1 expressed on HEVs in GALT and could potentially serve as an anti-inflammatory drug inhibiting GALT-specific lymphocyte migration.

  14. "Now he walks and walks, as if he didn't have a home where he could eat": food, healing, and hunger in Quechua narratives of madness.

    Orr, David M R

    2013-12-01

    In the Quechua-speaking peasant communities of southern Peru, mental disorder is understood less as individualized pathology and more as a disturbance in family and social relationships. For many Andeans, food and feeding are ontologically fundamental to such relationships. This paper uses data from interviews and participant observation in a rural province of Cuzco to explore the significance of food and hunger in local discussions of madness. Carers' narratives, explanatory models, and theories of healing all draw heavily from idioms of food sharing and consumption in making sense of affliction, and these concepts structure understandings of madness that differ significantly from those assumed by formal mental health services. Greater awareness of the salience of these themes could strengthen the input of psychiatric and psychological care with this population and enhance knowledge of the alternative treatments that they use. Moreover, this case provides lessons for the global mental health movement on the importance of openness to the ways in which indigenous cultures may construct health, madness, and sociality. Such local meanings should be considered by mental health workers delivering services in order to provide care that can adjust to the alternative ontologies of sufferers and carers.

  15. Duplication and diversification of the LEAFY HULL STERILE1 and Oryza sativa MADS5 SEPALLATA lineages in graminoid Poales

    Christensen Ashley R

    2012-02-01

    Full Text Available Abstract Background Gene duplication and the subsequent divergence in function of the resulting paralogs via subfunctionalization and/or neofunctionalization is hypothesized to have played a major role in the evolution of plant form. The LEAFY HULL STERILE1 (LHS1 SEPALLATA (SEP genes have been linked with the origin and diversification of the grass spikelet, but it is uncertain 1 when the duplication event that produced the LHS1 clade and its paralogous lineage Oryza sativa MADS5 (OSM5 occurred, and 2 how changes in gene structure and/or expression might have contributed to subfunctionalization and/or neofunctionalization in the two lineages. Methods Phylogenetic relationships among 84 SEP genes were estimated using Bayesian methods. RNA expression patterns were inferred using in situ hybridization. The patterns of protein sequence and RNA expression evolution were reconstructed using maximum parsimony (MP and maximum likelihood (ML methods, respectively. Results Phylogenetic analyses mapped the LHS1/OSM5 duplication event to the base of the grass family. MP character reconstructions estimated a change from cytosine to thymine in the first codon position of the first amino acid after the Zea mays MADS3 (ZMM3 domain converted a glutamine to a stop codon in the OSM5 ancestor following the LHS1/OSM5 duplication event. RNA expression analyses of OSM5 co-orthologs in Avena sativa, Chasmanthium latifolium, Hordeum vulgare, Pennisetum glaucum, and Sorghum bicolor followed by ML reconstructions of these data and previously published analyses estimated a complex pattern of gain and loss of LHS1 and OSM5 expression in different floral organs and different flowers within the spikelet or inflorescence. Conclusions Previous authors have reported that rice OSM5 and LHS1 proteins have different interaction partners indicating that the truncation of OSM5 following the LHS1/OSM5 duplication event has resulted in both partitioned and potentially novel gene

  16. LA POTENCIALIDAD DEL TERRITORIO EN LA RESTAURACIÓN ECOLÓGICA EL USO DE HERRAMIENTAS SIG PARA ESTABLECER PRIORIDADES DE RESTAURACIÓN ECOLÓGICA

    CORZO RAMÍREZ LILIANA; JERENA ERIK; RUBIO MENDOZA RODRIGO

    2012-01-01

    El presente artículo desarrolla una propuesta de diálogo interdisciplinar a través del uso de herramientas SIG (Sistemas de Información Geográfica) para la construcción de una metodología de identificación del Potencial de Restauración Ecológica (PRE), en áreas de borde urbano que hacen parte de la Estructura Ecológica Principal de Bogotá, según el ordenamiento territorial. Estas áreas suscriben problemáticas socio ambientales particulares propias de la interacción entre naturaleza y cultura....

  17. Determinação da fragilidade ambiental utilizando técnicas de suporte à decisão e SIG

    Donha,Annelissa G.; Souza,Luiz C. de P.; Sugamosto,Maria L.

    2006-01-01

    O trabalho objetivou determinar a fragilidade ambiental de uma área no município de Pinhais, região metropolitana de Curitiba, utilizando-se a técnica de avaliação por múltiplos critérios, presente no SIG Idrisi 32. Depois de gerados os mapas de classes de declividade, distância a partir dos rios, distância a partir das nascentes, distância a partir da represa, solos e uso atual, foram atribuídos pesos de importância para cada um dos fatores e através de Combinação Linear Ponderada geradas im...

  18. Integración de SIG con visualización 3D interactiva para la gestión y seguimiento de excavaciones arqueológicas

    Antonio José Seoane Nolasco

    2012-05-01

    Full Text Available Geographic Information Systems (GIS provide large information management capabilities. However, in many cases the feature of the visualization of this data over a huge detailed three-dimensional terrain does not exist or are limited, wich can be very useful or desirable in many applications. This paper presents the integration of SANTI, a three-dimensional visualization system of large terrain areas and geographical data, with gvSIG, a free GIS software. We describe the capabilities of the visualization system, the approach to connect the two systems and how it is applied to a project in development for the management of the excavation of the Castro de la Lanzada located at the Galician coast (Spain.

  19. State Implementation and Perceptions of Title I School Improvement Grants under the Recovery Act: One Year Later. Online Appendix--State Responses to Open-Ended Questions about the ARRA SIG Program

    Center on Education Policy, 2012

    2012-01-01

    To learn more about states' experiences with implementing school improvement grants (SIGs) funded through the American Recovery and Reinvestment Act (ARRA), the Center on Education Policy (CEP) administered a survey to state Title I directors. (Title I of the Elementary and Secondary Education Act provides federal funds to schools in low-income…

  20. The madness of crowds phenomenon in the collective decision-making by the cells, the cell's metacognition and cancer

    Saakian, David B.

    2018-02-01

    Recently it has been found that the collective decision-making in the group is efficient only when the confidences (a version of metacognition) of the members are similar, and it has been assumed that the metacognition (self-reference) in general is crucial for the human cooperation. Our goal is to map the decision making by the cells to decision making by humans, looking the analog of metacognition in the cells, accurately calculate the collective sensing of chemical gradients by the cells, and apply our results to cancer. We formulated the model for the chemeosensing by the cells with different diameters, solved it accurately and found that the collective chemosensing is very similar to the collective decision making by humans. We found that the collective sensing of the ligand concentration can be worse than for the most sensitive cell. We introduced the metacognition of the cells, and verify that the metacognition is impaired for the cancer case. We assume as a hypothesis that the impaired cell metacognition in case of cancer does not allow normal multi-cellularity, and cancer can arise when the "two heads are better than one" principle fails, and there is a "madness of crowds" phenomenon instead.

  1. Madness and the Middle Passage: Warner-Vierya's Juletane as a Paradigm for Writing Caribbean Women's Identities

    Ann Elizabeth Willey

    1997-06-01

    Full Text Available This article links Glissant's theory of an inherent Caribbean madness due to the originary rupture and alienation from Africa with Foucault's theory of the ritual significance and essential liminality of the madman as exemplified in the medieval figure of the "Ship of Fools." In calling the madman the "passenger par excellence," Foucault implies a connection between sanity and linear narratives, such as that of a voyage. Myriam Warner-Vierya's novel, Juletane , suggests that European paradigms of narrative and voyage are inadequate to provide a sense of self for Caribbean women. The novel takes the form of a diary that chronicles the steadily disintegrating marriage and sanity of Juletane, a Caribbean woman orphaned at ten, raised in Paris, who meets and marries an African student in an effort to find a new identity in her husband and a return to Africa. The novel suggests that the linear narrative of a return to the source in Africa denies the rupture with Africa and the immediate circumstances of Caribbean subject-hood. In her efforts, Juletane ignores the alternative narrative possibilities that the novel presents in the circular and shared narratives of women that do not depend on a sense of closure or arrival, but like the voyage of the madman, remain open to the infinite crossroads of the sea and narration.

  2. Motifs of Madness, Indifference, and Cannibalism as Symbols of a Depraved Society in Lu Xun's Short Stories

    Tina Ilgo

    2011-07-01

    Full Text Available This article analyzes two short stories by Lu Xun from his collection Outcry, which came into being at the culmination of the Chinese spiritual rebirth between 1818 and 1922. In “Diary of a Madman” and “The True Story of A Q” the author expresses his conviction that the existing system’s depravity produces “cannibalism,” causes a gradual decline in humanity, and exposes the main defects of human character. The impossibility of destroying the “iron house,” or people’s incapacity to change their “cannibalistic” nature, causes the loss of hope on the side of the “madmen” . It forces them to give up their insightfull knowledge and adapt to the majority. With the repetition of motifs such as “madness,” “indifference,” and “cannibalism,” which constantly recur in Lu Xun’s short stories, the author expressed his vision of traditional Chinese society and his pessimism about the future. At the same time these motifs reflect the author’s state of mind and his everlasting journey between hope and despair, “madness” and “indifference,” and tradition and modernity. If the stories are read in the context of twentieth-century China they can be understood as a direct criticism of the established Chinese society, whose values and norms derive from Confucianism, but they also contain deep symbolic meaning that renders them timeless.

  3. Gender, Madness, Religion, and Iranian-American Identity: Observations on a 2006 Murder Trial in Williamsport, Pennsylvania

    Camron Michael Amin

    2017-08-01

    Full Text Available Using participant observation, oral history interviews, and a study of court transcripts, Internet chats, and press coverage of a 2006 murder trial of an Iranian-American man in Williamsport, Pennsylvania, we can better appreciate the dynamic intersection of ethnicity, religion, and gender in constructing the social identity of Iranian-Americans. Brian Hosayn Yasipour, who immigrated to the United States in 1969, was convicted of murder in the third degree for killing his four-year-old daughter in 2001 during a custody dispute with his estranged, Iranian-born wife. He managed to avoid the death penalty. Debates about his guilt in America hinged on assessments of his mental state at the time of the crime and this, in turn, hinged on debates about how normative his actions would have been in Iran. Until his arrest, Brian had led a highly mobile life—moving back and forth between America, where he lived as a Christian, and Iran, where he visited as a Muslim. Was he a calculating Iranian-Islamic patriarch, outraged at the defiance of his wife and the attitudes of American courts toward his paternal rights? Or was he, per the court transcripts, a “white Christian” and survivor of childhood rape back in Iran, who lapsed into madness under the strain of his second divorce? Brian actively blurred these issues in court appearances before and after the murder—often expressing his agency in terms of preserving his imaginary and physical mobility.

  4. Madness in the movies: An evaluation of the use of cinema to explore mental health issues in nurse education.

    McCann, Edward; Huntley-Moore, Sylvia

    2016-11-01

    The research literature on the use of cinema in nurse education is relatively small. This study evaluates student nurses' learning experiences of a new undergraduate elective module called Madness in the Movies. Ethical approval was granted to conduct the study. Data were collected through an online survey and a social media discussion forum. The anonymous online survey responses were collated via Survey Monkey. Content analysis was conducted on the data from the Facebook discussion threads to understand, interpret and conceptualise the meanings from the data. All study participants agreed that their understanding of mental health issues was enriched, their attitudes and beliefs enhanced and their confidence to talk about mental health concerns increased significantly. This module provides a fruitful approach to encourage critical reflection on mental health issues in a safe environment that closely mirrors authentic practice experiences. The module facilitates the development of students' knowledge, values and attitudes in relation to person-centred mental healthcare. Copyright © 2016 Elsevier Ltd. All rights reserved.

  5. Soybean yield estimation by an agrometeorological model in a GIS Produtividade de soja estimada por modelo agrometeorológico num SIG

    Luciana Miura Sugawara Berka

    2003-01-01

    Full Text Available Agrometeorological models interfaced with the Geographic Information System - GIS are an alternative to simulate and quantify the effect of weather spatial and temporal variability on crop yield. The objective of this work was to adapt and interface an agrometeorological model with a GIS to estimate soybean [Glycine max (L. Merr.] yield. Yield estimates were generated for 144 municipalities in the State of Paraná, Brazil, responsible for 90% of the soybean production in the State, from 1996/1997 to 2000/2001. The model uses agronomical parameters and meteorological data to calculate maximum yield which will be penalized under drought stress. Comparative analyses between the yield estimated by the model and that reported by the Paraná State Department of Agriculture (SEAB were performed using the "t" test for paired observations. For the 1996/1997 year the model overestimated yield by 10.8%, which may be attributed to the occurrence of fungal diseases not considered by the model. For 1997/1998, 1998/1999 and 1999/2000 no differences (P > 0.05 were found between the yield estimated by the model and SEAB's data. For 2000/2001 the model underestimated yield by 10.5% and the cause for this difference needs further investigation. The model interfaced with a GIS is an useful tool to monitor soybean crop during growing season to estimate crop yield.Os modelos agrometeorológicos integrados em Sistemas de Informação Geográfica - SIG são uma alternativa para simular e quantificar o efeito da variabilidade espacial e temporal do clima sobre a produtividade agrícola. O objetivo deste trabalho foi adaptar e integrar um modelo agrometeorológico num SIG para estimar a produtividade da soja [Glycine max (L. Merr.]. Foram geradas estimativas de produtividade para 144 municípios do Estado do Paraná, responsáveis por 90% da produção de soja no Estado, em cinco anos-safra no período de 1996/1997 a 2000/2001. O modelo utiliza parâmetros agronômicos e

  6. Do successful agers live longer? The Vitality 90+ study.

    Nosraty, Lily; Enroth, Linda; Raitanen, Jani; Hervonen, Antti; Jylhä, Marja

    2015-02-01

    To discover whether successful aging, understood as a multidimensional concept, predicts further survival in very old people. The population sample consisted of 1,370 persons aged 90 or over in the Vitality 90+ study. Four alternative models of successful aging were constructed, each of them consisting of physical, psychological, and social dimensions. Mortality was followed up after 4 and 7 years. Three out of four models significantly predicted survival at both follow-ups for the whole group. Separately, "success" in the physical, psychological, and social components was also associated with higher survival. The associations were stronger in women than in men. Successful aging, measured using physical, psychological, and social dimensions, predicts the length of future life in nonagenarians. © The Author(s) 2014.

  7. Awareness of risk factors for loneliness among third agers

    Schoenmakers, E.C.; van Tilburg, T.G.; Fokkema, T.

    2014-01-01

    Awareness of risk factors for loneliness is a prerequisite for preventive action. Many risk factors for loneliness have been identified. This paper focuses on two: poor health and widowhood. Preventive action by developing a satisfying social network requires time and effort and thus seems

  8. Villas and farmsteads in the ager setinus (Sezze, Italy)

    Attema, Peter; de Haas, Tymon; Tol, Gijs

    2014-01-01

    This article presents and discusses the ceramic data of an archaeological surface survey carried out in 1994 by the Pontine Region Project (PRP) in the agricultural territory of the Roman Republican colony of Setia in the Pontine plain c. 80 km SE of Rome. While chronological distribution maps were

  9. Characterization and Functional Analysis of Five MADS-Box B Class Genes Related to Floral Organ Identification in Tagetes erecta.

    Ye Ai

    Full Text Available According to the floral organ development ABC model, B class genes specify petal and stamen identification. In order to study the function of B class genes in flower development of Tagetes erecta, five MADS-box B class genes were identified and their expression and putative functions were studied. Sequence comparisons and phylogenetic analyses indicated that there were one PI-like gene-TePI, two euAP3-like genes-TeAP3-1 and TeAP3-2, and two TM6-like genes-TeTM6-1 and TeTM6-2 in T. erecta. Strong expression levels of these genes were detected in stamens of the disk florets, but little or no expression was detected in bracts, receptacles or vegetative organs. Yeast hybrid experiments of the B class proteins showed that TePI protein could form a homodimer and heterodimers with all the other four B class proteins TeAP3-1, TeAP3-2, TeTM6-1 and TeTM6-2. No homodimer or interaction was observed between the euAP3 and TM6 clade members. Over-expression of five B class genes of T. erecta in Nicotiana rotundifolia showed that only the transgenic plants of 35S::TePI showed altered floral morphology compared with the non-transgenic line. This study could contribute to the understanding of the function of B class genes in flower development of T. erecta, and provide a theoretical basis for further research to change floral organ structures and create new materials for plant breeding.

  10. A novel MADS-box gene subfamily with a sister-group relationship to class B floral homeotic genes.

    Becker, A; Kaufmann, K; Freialdenhoven, A; Vincent, C; Li, M-A; Saedler, H; Theissen, G

    2002-02-01

    Class B floral homeotic genes specify the identity of petals and stamens during the development of angiosperm flowers. Recently, putative orthologs of these genes have been identified in different gymnosperms. Together, these genes constitute a clade, termed B genes. Here we report that diverse seed plants also contain members of a hitherto unknown sister clade of the B genes, termed B(sister) (B(s)) genes. We have isolated members of the B(s) clade from the gymnosperm Gnetum gnemon, the monocotyledonous angiosperm Zea mays and the eudicots Arabidopsis thaliana and Antirrhinum majus. In addition, MADS-box genes from the basal angiosperm Asarum europaeum and the eudicot Petunia hybrida were identified as B(s) genes. Comprehensive expression studies revealed that B(s) genes are mainly transcribed in female reproductive organs (ovules and carpel walls). This is in clear contrast to the B genes, which are predominantly expressed in male reproductive organs (and in angiosperm petals). Our data suggest that the B(s) genes played an important role during the evolution of the reproductive structures in seed plants. The establishment of distinct B and B(s) gene lineages after duplication of an ancestral gene may have accompanied the evolution of male microsporophylls and female megasporophylls 400-300 million years ago. During flower evolution, expression of B(s) genes diversified, but the focus of expression remained in female reproductive organs. Our findings imply that a clade of highly conserved close relatives of class B floral homeotic genes has been completely overlooked until recently and awaits further evaluation of its developmental and evolutionary importance. Electronic supplementary material to this paper can be obtained by using the Springer Link server located at http://dx.doi.org/10.1007/s00438-001-0615-8.

  11. Cálculo de la distribución espacial de la insolación potencial en el terreno empleando MDE en un ambiente SIG

    Alberto Gómez Tagle Chávez

    2012-02-01

    Full Text Available Se presenta un estudio de caso donde se calcula la insolación potencial (IP y la insolación real (IR para un área pequeña (634.4 ha empleando los programas Insol_Día e Insol_Mes a partir de un modelo digital de elevaciones (MDE. Estos programas calculan la IP utilizando la ubicación geográfica de la cuenca y el MDE mediante procedimientos de sistemas de información geográfica (SIG y ecuaciones de geometría esférica. La IR se obtuvo empleando los mapas digitales de IP calculada y corrigiendo los datos con información del observatorio meteorológico (15-0040, Morelia, Mich. del Servicio Meteorológico Nacional. Los resultados son una serie de mapas digitales dentro de un SIG que permiten conocer la distribución espacial de la IP y la IR en esta zona, para fechas definidas, meses enteros, estaciones y totales anuales. La IP anual máxima fue de 4 389 horas, mientras que la mínima fue de 2 946 horas. Los valores de IR máxima y mínima fueron 2 144.9 y 1 439.7 horas. Los promedios diarios anuales de IP máximo y mínimo fueron de 12.05 hr/día y 8.07 hr/día, siendo para IR de 5.88 hr/día y 3.944 hr/día, indicando una gran diferencia entre la IP y la IR. Se considera que este tipo de información puede ser de gran utilidad en estudios de distribución de la vegetación, balances hidrológicos, distribución de propiedades edáficas, velocidad de intemperismo de los materiales geológicos, arquitectura bioclimática y otras áreas donde la distribución espacial de la insolación se considere como una variable importante.

  12. Expression of paralogous SEP-, FUL-, AG- and STK-like MADS-box genes in wild-type and peloric Phalaenopsis flowers.

    Roberta eAcri-Nunes-Miranda

    2014-03-01

    Full Text Available The diverse flowers of Orchidaceae are the result of several major morphological transitions, among them the most studied is the differentiation of the inner median tepal into the labellum, a perianth organ key in pollinator attraction. Type A peloria lacking stamens and with ectopic labella in place of inner lateral tepals are useful for testing models on the genes specifying these organs by comparing their patterns of expression between wild-type and peloric flowers. Previous studies focused on DEFICIENS and GLOBOSA-like MADS-box genes because of their conserved role in perianth and stamen development. The ‘orchid code’ model summarizes this work and shows in Orchidaceae there are four paralogous lineages of DEFICIENS/AP3-like genes differentially expressed in each floral whorl. Experimental tests of this model showed the conserved, higher expression of genes from two specific DEF-like gene lineages is associated with labellum development. The present study tests whether eight MADS-box candidate SEP-, FUL-, AG- and STK-like genes have been specifically duplicated in the Orchidaceae and are also differentially expressed in association with the distinct flower organs of Phalaenopsis hyb. Athens. The gene trees indicate orchid-specific duplications. In a way analogous to what is observed in labellum-specific DEF-like genes, a two-fold increase in the expression of SEP3-like gene PhaMADS7 was measured in the labellum-like inner lateral tepals of peloric flowers. The overlap between SEP3-like and DEF-like genes suggests both are associated with labellum specification and similar positional cues determine their domains of expression. In contrast, the uniform messenger levels of FUL-like genes suggest they are involved in the development of all organs and their expression in the ovary suggests cell differentiation starts before pollination. As previously reported AG-like and STK-like are exclusively expressed in gynostemium and ovary, however no

  13. Expression of paralogous SEP-, FUL-, AG- and STK-like MADS-box genes in wild-type and peloric Phalaenopsis flowers.

    Acri-Nunes-Miranda, Roberta; Mondragón-Palomino, Mariana

    2014-01-01

    The diverse flowers of Orchidaceae are the result of several major morphological transitions, among them the most studied is the differentiation of the inner median tepal into the labellum, a perianth organ key in pollinator attraction. Type A peloria lacking stamens and with ectopic labella in place of inner lateral tepals are useful for testing models on the genes specifying these organs by comparing their patterns of expression between wild-type and peloric flowers. Previous studies focused on DEFICIENS- and GLOBOSA-like MADS-box genes because of their conserved role in perianth and stamen development. The "orchid code" model summarizes this work and shows in Orchidaceae there are four paralogous lineages of DEFICIENS/AP3-like genes differentially expressed in each floral whorl. Experimental tests of this model showed the conserved, higher expression of genes from two specific DEF-like gene lineages is associated with labellum development. The present study tests whether eight MADS-box candidate SEP-, FUL-, AG-, and STK-like genes have been specifically duplicated in the Orchidaceae and are also differentially expressed in association with the distinct flower organs of Phalaenopsis hyb. "Athens." The gene trees indicate orchid-specific duplications. In a way analogous to what is observed in labellum-specific DEF-like genes, a two-fold increase in the expression of SEP3-like gene PhaMADS7 was measured in the labellum-like inner lateral tepals of peloric flowers. The overlap between SEP3-like and DEF-like genes suggests both are associated with labellum specification and similar positional cues determine their domains of expression. In contrast, the uniform messenger levels of FUL-like genes suggest they are involved in the development of all organs and their expression in the ovary suggests cell differentiation starts before pollination. As previously reported AG-like and STK-like genes are exclusively expressed in gynostemium and ovary, however no evidence for

  14. Overcoming cisplatin resistance in non-small cell lung cancer with Mad2 silencing siRNA delivered systemically using EGFR-targeted chitosan nanoparticles.

    Nascimento, Ana Vanessa; Singh, Amit; Bousbaa, Hassan; Ferreira, Domingos; Sarmento, Bruno; Amiji, Mansoor M

    2017-01-01

    Efficiency of chemotherapy is often limited by low therapeutic index of the drug as well as emergence of inherent and acquired drug resistance in cancer cells. As a common strategy to overcome drug resistance, higher doses of chemo-agents are administered. However, adverse side effects are usually increased as a consequence. A potentially effective approach is to combine chemotherapy with other therapeutic strategies such as small interfering RNAs (siRNAs) that allow the use of lower yet efficient doses of the anticancer drugs. We previously developed epidermal growth factor receptor (EGFR)-targeted chitosan (CS) nanoparticles as a versatile delivery system for silencing the essential mitotic checkpoint gene Mad2, and induce cell death. Here, we tested this system as a single therapy and in combination with cisplatin in cisplatin sensitive and resistant lung cancer models, and characterized its in vivo efficacy and safety. Combination treatment resulted in significant improvement in tumor inhibition that was strikingly more effective in cisplatin-resistant tumors. Importantly, effective cisplatin dosage was dramatically reduced in the co-therapy regimen resulting in negligible toxic effects from the drug as confirmed by parameters such as body weight gain, biochemical markers of hepatic and renal function, and histopathology of liver/kidney/spleen tissues. Overall, we demonstrate that the combination of Mad2 siRNA-loaded CS nanoparticles strategy with chemotherapeutic agents such as cisplatin constitutes an efficient and safe approach for the treatment of drug resistant tumors. Lung cancer remains one of the leading killers in the United States and around the world. Platinum agents, including cisplatin, are the first line treatment in lung cancer, including non-small cell lung cancer (NSCLC), which is the predominant form of lung cancer. In this study, we have evaluated Mad2 cell-cycle checkpoint gene silencing using small interfering RNA (siRNA) delivered

  15. Studies of tt+cc production with MadGraph5_aMC@NLO and Herwig++ for the ATLAS experiment

    The ATLAS collaboration

    2016-01-01

    Studies of the simulation of ttcc at the LHC, at a centre-of-mass energy of 13 TeV, based on next-to-leading order calculations with MadGraph5_aMC@NLO matched to Herwig++ are presented. Samples with ttcc in the three flavour scheme, in a next-to-leading order matrix element are compared to an inclusive tt sample with the charm contribution coming from the parton shower. The effects of generator-level cuts, the functional form of the renormalization and factorization scales, and the starting scale of the parton shower are investigated.

  16. A loucura como questão semântica: uma interpretação kantiana Madness as a semantic question: a Kantian interpretation

    Daniel Omar Perez

    2009-01-01

    Full Text Available Apesar das mudanças do projeto kantiano, é possível identificar o problema da loucura como sendo abordado em duas perspectivas: uma fisiológica, outra semântica. A abordagem fisiológica corresponde ao modelo das ciências dos objetos dos sentidos externos. Já a abordagem semântica da loucura se desenvolve dentro da tarefa crítica da filosofia, isto é, como parte de uma investigação acerca do alcance e dos limites da razão humana. Nesse sentido, a loucura se insere em duas séries diferentes. No primeiro caso aparece vinculada às lesões cerebrais, problemas de percepção ou até mesmo em relação ao consumo de substâncias que alteram o funcionamento físico. No segundo caso se relaciona com o entusiasmo do desvario profético, o fanatismo religioso, o misticismo e até mesmo a ilusão metafísica. Para desenvolver o nosso trabalho apresentaremos elementos da abordagem fisiológica e da abordagem semântica encontradas em alguns dos diferentes textos e, por último, realizaremos algumas considerações sobre a possibilidade do desenvolvimento de um saber sobre a loucura em Kant.Despite of the changes in the kantian project is possible to identify the problem of the madness as being boarded in two perspectives: 1. physiologic, 2. semantic. The physiologic approach corresponds to the model of the sciences about objects of the external senses. The semantic approach of the madness develops inside of the critical task of the philosophy, that is, as part of an investigation concerning the reach and the limits of the human reason. In this sense, the madness is in two different series. In the first case it appears entailed to the cerebral lesions, problems of perception or with the consumption of substances that change the physical operation. In the second case it relates with enthusiasm prophetic, the religious fanaticism, mysticism and even with the metaphysic. To develop our work here will present elements of the physiologic approach

  17. Addendum to the Closure Report for Corrective Action Unit 113: Area 25 R-MAD Facility, Nevada National Security Site, Nevada

    2011-01-01

    This addendum to the Closure Report for Corrective Action Unit 113: Area 25, Reactor Maintenance, Assembly, and Disassembly Facility, Building 3110, Nevada Test Site, Nevada, DOE/NV--891-VOL I-Rev. 1, dated July 2003, provides details of demolition, waste disposal, and use restriction (UR) modification for Corrective Action Unit 113, Area 25 R-MAD Facility. Demolition was completed on July 15, 2010, when the last of the building debris was disposed. Final field activities were concluded on August 30, 2010, after all equipment was demobilized and UR signs were posted. This work was funded by the American Recovery and Reinvestment Act.

  18. Effect of food-related stress conditions and loss of agr and sigB on seb promoter activity in S. aureus.

    Sihto, Henna-Maria; Stephan, Roger; Engl, Christoph; Chen, John; Johler, Sophia

    2017-08-01

    Staphylococcal enterotoxin B (SEB) causes staphylococcal food poisoning and is produced in up to ten times higher quantities than other major enterotoxins. While Staphylococcus aureus growth is often repressed by competing flora, the organism exhibits a decisive growth advantage under some stress conditions. So far, data on the influence of food-related stressors and regulatory mutations on seb expression is limited and largely based on laboratory strains, which were later reported to harbor mutations. Therefore, the aim of this study was to investigate the influence of stress and regulatory mutations on seb promoter activity. To this end, transcriptional fusions were created in two strains, USA300 and HG003, carrying different seb upstream sequences fused to a blaZ reporter. NaCl, nitrite, and glucose stress led to significantly decreased seb promoter activity, while lactic acid stress resulted in significantly increased seb promoter activity. Loss of agr decreased seb promoter activity and loss of sigB increased promoter activity, with the magnitude of change depending on the strain. These results demonstrate that mild stress conditions encountered during food production and preservation can induce significant changes in seb promoter activity. Copyright © 2017 Elsevier Ltd. All rights reserved.

  19. Improvement of iturin A production in Bacillus subtilis ZK0 by overexpression of the comA and sigA genes.

    Zhang, Z; Ding, Z T; Zhong, J; Zhou, J Y; Shu, D; Luo, D; Yang, J; Tan, H

    2017-06-01

    Bacillus subtilis ZK0, which was isolated from cotton, produces a type of lipopeptide antibiotic iturin A that inhibits the growth of pathogenic fungi on agricultural crops. However, the low level of iturin A production by B. subtilis ZK0 does not support its large-scale application. In this study, B. subtilis ZK0 was subjected to genetic manipulation to improve iturin A production. By the independent or simultaneous overexpression of two regulatory genes (comA and sigA), iturin A production by B. subtilis ZK0 was significantly increased. When both genes were simultaneously overexpressed under optimal conditions, iturin A production increased up to 215 mg l -1 (an approximate 43-fold increase compared with B. subtilis ZK0). Moreover, overexpression of both genes was unexpectedly found to inhibit biofilm formation by B. subtilis ZK0, indicating that the phenomenon of 'stuck fermentation' would be avoided during B. subtilis ZK0 fermentation. In conclusion, a genetic manipulation method that improves iturin A production and inhibits biofilm formation in B. subtilis ZK0 is reported for the first time and this method has the potential to be widely applied in B. subtilis ZK0 commercial fermentation. This study provides new perspectives on improving iturin A production by Bacillus subtilis. Our newly engineered strains could be applied to commercial fermentation by enhancing yields of iturin A and reducing the rate of 'stuck fermentation'. Increased production would facilitate more widespread application of this powerful antibiotic. © 2017 The Society for Applied Microbiology.

  20. Una representación basada en Semat y RUP para el Método de Desarrollo SIG del Instituto Geográfico Agustín Codazzi

    Claudia Elena Durango Vanegas

    2015-06-01

    Full Text Available Los Sistemas de Información Geográfica (SIG constituyen un apoyo importante para el análisis y solución de problemas en áreas, como: salud, hidrología, ingeniería y gestión territorial, entre otras. El desarrollo metodológico de los SIG no incluye especificaciones propias; por el contrario, son propuestas modificadas de otros métodos, que contienen los mismos productos de trabajo, actividades y recursos de los métodos tradicionales de desarrollo, tales como el Rational Unified Process (RUP. Uno de esos métodos es del Instituto Geográfico Agustín Codazzi (IGAC. Semat (Teoría y Métodos de la Ingeniería de Software es una iniciativa que ayuda a capturar elementos esenciales y comunes, universales a todos los esfuerzos de desarrollo de software. En busca de las especificaciones propias de los métodos de desarrollo de SIGs, en este artículo se propone una representación basada en el núcleo de Semat del método IGAC para identificar la relación con el método tradicional Proceso Unificado de Racional (RUP. Con esta representación es posible determinar las prácticas asociadas con el método IGAC que se heredan de los métodos tradicionales.

  1. [A study on the construction, expression and immunosterility of Lagurus laguru zona pellucida 3 DNA vaccine pVAX1-sig-LTB-lZP3-C3d3].

    Li, Chen-Chen; Yu, Ji-Yun; Jiang, Min; Tu, Yi-Xian; Ma, Xiao-Lin; Zhang, Fu-Chun

    2011-09-01

    To enhance the immunocontraceptive effect of Lagurus lagurus zona pellucida 3 DNA vaccine, and to achieve the prospect of application through the pVAX1-sig-LTB-lZP3-C3d3 different immunity pathway. Two adjuvant molecules were constructed into the recombinant plasmid pVAX1-sig-LTB-lZP3-C3d3 as DNA vaccine which contains Escherichia coli heat-labile enterotoxin B subunit and the molecular adjuvant 3 copies of C3d. The results of RT-PCR and western blot showed that the DNA vaccine was expressed in mRNA and protein level. The female C57BL/6 mice were immunized by three ways: intramuscular injection, intranasal or oral route.Antibody levels and types were detected by ELISA. ELISA results showed that recombinant plasmid pVAX1-sig-LTB-lZP3-C3d3 immunization induced specific IgG, IgA levels were significantly different comparing with control (Psig-LTB-lZP3-C3d3 can induce the specific immune response efficiently and enhance the immunocontraceptive effects.

  2. A Novel Sucrose-Regulatory MADS-Box Transcription Factor GmNMHC5 Promotes Root Development and Nodulation in Soybean (Glycine max [L.] Merr.

    Wei Liu

    2015-08-01

    Full Text Available The MADS-box protein family includes many transcription factors that have a conserved DNA-binding MADS-box domain. The proteins in this family were originally recognized to play prominent roles in floral development. Recent findings, especially with regard to the regulatory roles of the AGL17 subfamily in root development, have greatly broadened their known functions. In this study, a gene from soybean (Glycine max [L.] Merr., GmNMHC5, was cloned from the Zigongdongdou cultivar and identified as a member of the AGL17 subfamily. Real-time fluorescence quantitative PCR analysis showed that GmNMHC5 was expressed at much higher levels in roots and nodules than in other organs. The activation of expression was first examined in leaves and roots, followed by shoot apexes. GmNMHC5 expression levels rose sharply when the plants were treated under short-day conditions (SD and started to pod, whereas low levels were maintained in non-podding plants under long-day conditions (LD. Furthermore, overexpression of GmNMHC5 in transgenic soybean significantly promoted lateral root development and nodule building. Moreover, GmNMHC5 is upregulated by exogenous sucrose. These results indicate that GmNMHC5 can sense the sucrose signal and plays significant roles in lateral root development and nodule building.

  3. A STE12 homologue of the homothallic ascomycete Sordaria macrospora interacts with the MADS box protein MCM1 and is required for ascosporogenesis.

    Nolting, Nicole; Pöggeler, Stefanie

    2006-11-01

    The MADS box protein MCM1 controls diverse developmental processes and is essential for fruiting body formation in the homothallic ascomycete Sordaria macrospora. MADS box proteins derive their regulatory specificity from a wide range of different protein interactions. We have recently shown that the S. macrospora MCM1 is able to interact with the alpha-domain mating-type protein SMTA-1. To further evaluate the functional roles of MCM1, we used the yeast two-hybrid approach to identify MCM1-interacting proteins. From this screen, we isolated a protein with a putative N-terminal homeodomain and C-terminal C2/H2-Zn2+ finger domains. The protein is a member of the highly conserved fungal STE12 transcription factor family of proteins and was therefore termed STE12. Furthermore, we demonstrate by means of two-hybrid and far western analysis that in addition to MCM1, the S. macrospora STE12 protein is able to interact with the mating-type protein SMTA-1. Unlike the situation in the closely related heterothallic ascomycete Neurospora crassa, deletion (Delta) of the ste12 gene in S. macrospora neither affects vegetative growth nor fruiting body formation. However, ascus and ascospore development are highly impaired by the Deltaste12 mutation. Our data provide another example of the functional divergence within the fungal STE12 transcription factor family.

  4. Safety assessment document for spent fuel handling, packaging, and storage demonstrations at the E-MAD facility on the Nevada Test Site

    1985-04-01

    The objectives for spent fuel handling and packaging demonstration are to develop the capability to satisfactorily encapsulate typical commercial nuclear reactor spent fuel assemblies and to establish the suitability of interim dry surface and near surface storage concepts. To accomplish these objectives, spent fuel assemblies from a pressurized water reactor have been received, encapsulated in steel canisters, and emplaced in on-site storage facilities and subjected to other tests. As an essential element of these demonstrations, a thorough safety assessment of the demonstration activities conducted at the E-MAD facility has been completed. This document describes the site location and characteristics, the existing E-MAD facility, and the facility modifications and equipment additions made specifically for the demonstrations. The document also summarizes the Quality Assurance Program utilized, and specifies the principal design criteria applicable to the facility modifications, equipment additions, and process operations. Evaluations have been made of the radiological impacts of normal operations, abnormal operations, and postulated accidents. Analyses have been performed to determine the affects on nuclear criticality safety of postulated accidents and credible natural phenomena. The consequences of postulated accidents resulting in fission product gas release have also been estimated. This document identifies the engineered safety features, procedures, and site characteristics that (1) prevent the occurrence of potential accidents or (2) assure that the consequences of postulated accidents are either insignificant or adequately mitigated

  5. Corrective Action Decision Document for Corrective Action Unit 254: Area 25 R-MAD Decontamination Facility, Nevada Test Site, Nevada

    2000-01-01

    This Corrective Action Decision Document identifies and rationalizes the US Department of Energy, Nevada Operations Office's selection of a recommended corrective action alternative (CAA) appropriate to facilitate the closure of Corrective Action Unit (CAU) 254, R-MAD Decontamination Facility, under the Federal Facility Agreement and Consent Order. Located in Area 25 at the Nevada Test Site in Nevada, CAU 254 is comprised of Corrective Action Site (CAS) 25-23-06, Decontamination Facility. A corrective action investigation for this CAS as conducted in January 2000 as set forth in the related Corrective Action Investigation Plan. Samples were collected from various media throughout the CAS and sent to an off-site laboratory for analysis. The laboratory results indicated the following: radiation dose rates inside the Decontamination Facility, Building 3126, and in the storage yard exceeded the average general dose rate; scanning and static total surface contamination surveys indicated that portions of the locker and shower room floor, decontamination bay floor, loft floor, east and west decon pads, north and south decontamination bay interior walls, exterior west and south walls, and loft walls were above preliminary action levels (PALs). The investigation-derived contaminants of concern (COCs) included: polychlorinated biphenyls, radionuclides (strontium-90, niobium-94, cesium-137, uranium-234 and -235), total volatile and semivolatile organic compounds, total petroleum hydrocarbons, and total Resource Conservation and Recovery Act (Metals). During the investigation, two corrective action objectives (CAOs) were identified to prevent or mitigate human exposure to COCs. Based on these CAOs, a review of existing data, future use, and current operations at the Nevada Test Site, three CAAs were developed for consideration: Alternative 1 - No Further Action; Alternative 2 - Unrestricted Release Decontamination and Verification Survey; and Alternative 3 - Unrestricted

  6. LOS SISTEMAS DE INFORMACIÓN GEOGRÁFICA (SIG, UNA GRAN HERRAMIENTA PARA LA SILVICULTURA URBANA GEOGRAPHICAL INFORMATION SYSTEMS (GIS, A GREAT TOOL FOR URBAN SYLVICULTURE

    Leodán Andrés Otaya Burbano

    2006-06-01

    Full Text Available Como una fase piloto, para adquirir experiencia, definir metodologías y determinar las ventajas de los Sistemas de Información Geográfica (SIG al ser aplicados en la Silvicultura Urbana, se realizó el inventario, diagnóstico, propuesta de manejo y valoración económica del Bosque Urbano del barrio La Magnolia del Municipio de Envigado, Departamento de Antioquia, Colombia. Para el manejo y análisis de los registros recolectados en campo, se diseñó una base de datos en el software Microsoft Access®. Se ubicaron las especies inventariadas en mapas digitales y se analizó la condición de las mismas por medio de algunas herramientas y extensiones de la arquitectura tecnológica ArcGISÒ 8.3 en cuanto a: características, tratamientos silviculturales requeridos y conflictos con el entorno. Como resultado se determinó que el SIG del Bosque Urbano realizado para un barrio en particular se convierte en una herramienta que le permite a las autoridades ambientales y a investigadores interesados, tener acceso fácil y ágil de la información almacenada en él; permite realizar la programación de las actividades silviculturales requeridas, tener una visión general del Bosque Urbano de acuerdo a la infraestructura del barrio, apoyada con las fotografías del sector, para una mayor ilustración; permite además la inclusión o eliminación de información de una manera rápida y sencilla, facilitando así la toma de decisiones con relación al manejo del arbolado urbano y a la comparación con otros estudios similares.As a pilot phase, to acquire experience, define methodologies, and determine the advantages of Geographical Information Systems (GIS for applying to Urban Silviculture, an inventory, diagnosis, management plan and economic appraisal were made for the Urban Forest in the Magnolia neighborhood of the Envigado Municipality, Department of Antioquia, Colombia. For the management and analysis of the data collected in field, a database

  7. La locura en la obra de Lacan: articulaciones con las nociones de cuerpo, manía y sexuación Madness in the Lacan work's: joints with the body, mania and sexuation

    Pablo D. Muñoz

    2009-12-01

    Full Text Available En este artículo se presentan algunos avances del proyecto de investigación P601 (2008-2010: "Variaciones del concepto de locura en la obra de J. Lacan. Su incidencia en el diagnóstico diferencial neurosis-psicosis". Con el objetivo de avanzar en la precisión de la formalización del concepto de locura en la enseñanza de Lacan, su traducción en términos clínicos, y ponderar el modo en que dicha formalización reordena las concepciones de la locura desplegadas anteriormente en la psiquiatría y el psicoanálisis, indagamos aquí la relación de la locura con los conceptos de cuerpo, manía y sexuación.We present in this work the state and forward of the project UBACyT 2008-2010 (P601: "Variations of the concept of madness in the work of J. Lacan. Its incidence in the diagnosis differential neurosis-psychosis". With the goal of specify the formalization of the madness concept in Lacan's work, its translation in clinical terms, and analyse the way in which this formalization reorganizes the previous conceptions of madness developed in psychiatry and psychoanalysis, we investigate here the relation of madness with the concepts of body, mania and sexuation.

  8. Diseño colaborativo de una plataforma SIG web de humedales urbanos del área Metropolitana de Concepción (urbancost

    Ivonne Andrea Rueda Seguel

    2018-05-01

    Full Text Available En el Área Metropolitana de Concepción existe una gran variedad de humedales altamente presionados por la urbanización, por tanto, se vuelve importante dar a conocer y disponer de indicadores espaciales capaces de generar datos que revelen el grado de naturalidad, biodiversidad y el efecto de los humedales urbanos. Desde esa perspectiva, se diseñó colaborativamente una plataforma Web-SIG con una interface y contenidos correspondientes a los requerimientos de los usuarios potenciales. A partir de encuestas aplicadas a la población, fue posible observar la necesidad de información sobre indicadores, diversidad de especies y servicios ecosistémicos asociados a estas áreas; por lo cual se realizó una revisión bibliográfica para integrar dichas sugerencias a la plataforma. En cuanto a la construcción, esta se llevó a cabo en el entorno de los Sistemas de Información Geográfica (ArcGIS online y luego se publicó como visor de mapas en una página web. Finalmente, la herramienta facilita la interacción con datos espaciales y contribuye a difundir investigaciones científicas en áreas de conflictos socioambientales en un lenguaje cercano a toda la comunidad. Se espera que la información sea de utilidad para los encargados de tomar decisiones en estos temas, como también para determinados actores locales y la comunidad a favor de la conservación de humedales urbanos.

  9. Impact of the Regulators SigB, Rot, SarA and sarS on the Toxic Shock Tst Promoter and TSST-1 Expression in Staphylococcus aureus.

    Diego O Andrey

    Full Text Available Staphylococcus aureus is an important pathogen manifesting virulence through diverse disease forms, ranging from acute skin infections to life-threatening bacteremia or systemic toxic shock syndromes. In the latter case, the prototypical superantigen is TSST-1 (Toxic Shock Syndrome Toxin 1, encoded by tst(H, and carried on a mobile genetic element that is not present in all S. aureus strains. Transcriptional regulation of tst is only partially understood. In this study, we dissected the role of sarA, sarS (sarH1, RNAIII, rot, and the alternative stress sigma factor sigB (σB. By examining tst promoter regulation predominantly in the context of its native sequence within the SaPI1 pathogenicity island of strain RN4282, we discovered that σB emerged as a particularly important tst regulator. We did not detect a consensus σB site within the tst promoter, and thus the effect of σB is likely indirect. We found that σB strongly repressed the expression of the toxin via at least two distinct regulatory pathways dependent upon sarA and agr. Furthermore rot, a member of SarA family, was shown to repress tst expression when overexpressed, although its deletion had no consistent measurable effect. We could not find any detectable effect of sarS, either by deletion or overexpression, suggesting that this regulator plays a minimal role in TSST-1 expression except when combined with disruption of sarA. Collectively, our results extend our understanding of complex multifactorial regulation of tst, revealing several layers of negative regulation. In addition to environmental stimuli thought to impact TSST-1 production, these findings support a model whereby sporadic mutation in a few key negative regulators can profoundly affect and enhance TSST-1 expression.

  10. Los microclimas térmicos urbanos y áreas verdes en la ciudad de Sancti Spíritus. Los SIG en el planeamiento.

    Félix D. Hernández Rubio

    2008-07-01

    Full Text Available La presente investigación es originada por la necesidad en la ciudad de Sancti Spíritus de un estudio ambiental, que interrelacione cómo ha influido el cambio climático en el clima urbano y la relación de este último con el sistema de áreas verdes de la ciudad, como elemento moderador del ambiente térmico a escala de planeamiento, urbana y arquitectónica, y que a su vez proporcionen elementos más precisos para la toma de decisiones y criterios en las acciones de rehabilitación e inserción que se realicen, o para zonas de nuevo desarrollo dentro de la ciudad. Se analiza el déficit de áreas verdes a nivel de zona tipomorfológica y de consejo popular, además se analizan los problemas del cambio climático, la isla de calor urbana, se definen las variables y parámetros que influyen sobre las condiciones microclimáticas térmicas (Coeficiente de Ocupación del Suelo. Pavimentación, y otros aspectos medioambientales. Posteriormente se realizan mediciones del complejo temperatura – humedad relativa del aire y velocidad del viento en 24 puntos de la ciudad, elaborándose mapas en Sistemas de Información Geográficos (SIG, Mapinfo 6.5 sobre el comportamiento del ambiente térmico y humedad relativa del aire en la ciudad, tema sobre el cual no se ha desarrollado ninguna investigación hasta el momento en la ciudad objeto de estudio y de las cuales es pionera el presente trabajo, aportándose nuevos enfoques y estrategias para estas labores, lo cual incorpora nuevos criterios en el Plan General de Ordenamiento Territorial y Urbano (PGOTU.

  11. Identificação dos níveis de degradação de matas ripárias com o uso de SIG

    Roseli Mendonça Dias

    Full Text Available O presente trabalho objetivou identificar níveis de degradação de matas ripárias com o emprego de Sistema de Informações Geográficas (SIG, para a determinação das categorias de degradação encontradas na Unidade de Gerenciamento de Recursos Hídricos do Tietê-Jacaré, no município de São Carlos-SP. O método consistiu na interpretação visual de imagens de satélite Landsat 5 da área de estudo, em amostragens de campo e no desenvolvimento de procedimentos para a classificação dos níveis de degradação de suas matas ripárias. Foi gerado um mapa das categorias de degradação das matas ripárias, o que permitiu avaliar os locais degradados, com floresta e em estágios de regeneração. A interpretação de imagens de satélite para a identificação dos níveis de degradação das matas ripárias apresenta-se como um instrumento útil para subsidiar informações sobre as matas ripárias das drenagens e nascentes de uma bacia hidrográfica. Outra possibilidade corresponde ao monitoramento do desenvolvimento da degradação ou da recuperação destas matas.

  12. Síntesis hidrogeológica y modelización regional de la Cuenca Media del Tajo asistida por un SIG

    Lucini, M.

    2001-04-01

    chosen confiuration ARC/INFO-IDRISIVISUAL MODFLOW has shown its usefulness and versatility to face the modelization of this area, which is characterized for its complexity and big extension.En el presente trabajo se exponen los resultados obtenidos de la aplicación de un Código Numérico de Modelización Hidrogeológica (CNMH asistido por un Sistema de Información Geográfica (SIG a un área de 20.952 km2 de la cuenca media del Tajo que incluye tanto a las depresiones terciarias del Campo Arañuelo y de Madrid, parcialmente, como al sustrato Igneo-metamórfico del macizo Ibérico hasta una profundidad de 5.500 m. Geográficamente incluye las ciudades de Madrid, Toledo y Talavera de la Reina; el accidente tectónico del Jerte y la confluencia entre el río Tajo y el Tiétar constituyen el área más occidental. Los objetivos del trabajo son, estudiar la problemática de integrar en un modelo hidrogeológico regional de gran escala formaciones de características hidrogeológicas muy distintas, analizar y definir una configuración SIG-CNMH para aplicar en el estudio, y avanzar en el conocimiento regional desde una perspectiva integradora y multidisciplinar. Se trabaja con un modelo isótropo y cinco anisótropos, estos últimos considerando la influencia en el flujo de tres familias de accidentes tectónicos -N 105, N 142.5 y N 168.75- y una esquistosidad media regional N 140. La calibración se basa en piezometría restituida y aportaciones base de la red hidrográfica. Se obtuvieron distintos valores de los parámetros calibrados según el modelo. En todos los casos la calidad del ajuste global es correcta y aceptable; sin embargo, cada uno de los modelos ofrece mejor calidad de ajuste respecto a los restantes en los sectores donde los rasgos que representan coinciden con los del sistema real. La evolución de la permeabilidad en profundidad resultante de la calibración es coherente con la esperada, siendo las relaciones kx/kz = ky/kz = 1, en los primeros 200 m y 0

  13. Revisors opklaring af besvigelser - Læren af praksis

    Bloch Christensen, Maria; Kristensen, Rikke Holmslykke; Warming-Rasmussen, Bent

    ansvarsfordelingen. Er det bestyrelsen, direktionen, controllerne eller navnlig den eksterne revisor, som burde have opdaget besvigelsen? Eller burde selskabets medarbejdere have påtaget sig det sociale ansvar at agere som whistleblowers? De berørte parter undskylder sig ofte ved, at den nu afslørede bedrager...... medarbejdere med socialt ansvar (whistleblowers), men også de eksterne, herunder kreditgivere, offentlige kontrolmyndigheder samt økonomiske journalister....

  14. Ratings Behaving Madly

    McCutcheon, Marion; Jensen, Pia Majbritt

    that of SBS’s total annual share for people aged 16 years and over, which grew at an average 0.4 per cent over the same time period. As well as attracting a growing audience, Danish dramas also tend to attract larger audiences than other foreign language dramas on SBS although they do not, of course, come...... occupied increasing amounts of screen time on Australian SBS’s television broadcast service, and attracted a growing audience, making Danish drama a vibrant offering in mainstream television in Australia. Australia and SBS thus stand out as the only country/broadcaster outside of Denmark’s wider geo...

  15. ["Stealing madness"--kleptomania].

    Polak, D M; Dannon, P N

    2001-06-01

    Kleptomania is one of the common less diagnosed impulse control disorders, which could be treated by the combination of psychological and pharmacological therapy. The most effective treatment regimens include cognitive behavioral therapy and interpersonal psychotherapy in the psychological field, and antidepressants and mood stabilizers as pharmacological treatment. Most of the patients with kleptomania are initially treated only for a comorbid psychiatric disorder, since kleptomaniac symptoms had not been raised in the anamnesis. The aim of our article is to inform the physicians about the possible diagnosis and treatment options for this disorder in order to prevent co-morbidity with other psychiatric disorders. Some specific cases are presented in order to explain symptomatology.

  16. M.A.D

    Kacunko, Slavko

    2009-01-01

    This essay seeks to delineate the possible mutual benefits that different disciplines such as art history, media studies, computer science, etc. might derive from their specific efforts at formulating requirements and strategies for the appraisal of records and data as well as scientific and othe...

  17. Atraktsioon atraktsioonis / Madli Vitismann

    Vitismann, Madli, 1950-

    2013-01-01

    Teadlaste öö Eesti Meremuuseumis uuris meremeeste hingeelu: vaadata sai näitust "Soolase elu kirjad", Professor Mati Heidmets vestles amatööreksperdi Marko Matverega ruumipsühholoogiast, Teadusajakirjanike Selts andis üle ajakirjandussõbralikuma teadlase auhinna "Ökul" Tarmo Soomerele. Paksus Margaretas saab vaadata laevapostkaartide näitust soome koguja Keijo Polóni kogust ja Lennusadamas USA näituseproduktsioonifirma Premier Exhibitionsi näitust "Titanic"

  18. Marijuana and madness

    Castle, David J; Murray, Robin, MD, M Phil, MRCP, MRC Psych; D'Souza, Deepak Cyril

    2012-01-01

    .... Effects of cannabis on mood are reviewed, as are its effects on cognition. This new edition has been extensively updated and expanded with 10 new chapters to incorporate major new research findings. This book will be of interest to all members of the mental health team, as well as to neuroscientists, epidemiologists, public health specialists and those involved in drug and alcohol research"--

  19. Normaalsuse uuringud / Madli Pesti

    Pesti, Madli, 1980-

    2010-01-01

    24.-27. septembrini Slovakkias Nitras toimunud rahvusvahelisest teatrifestivalist "Divadelna Nitra 2010". Pikemalt Dmitri Krõmovi lavastusest "Oopus nr 7", tantsulavastusest "Out of Context: For Pina" ("Kontekstist väljas, Pinale") koreograaf Alain Plateli trupi Les Ballets C de la B esituses ja Christoph Willibald Glucki ooperist "Orpheus ja Eurydike" Mariusz Trelinski lavastuses

  20. Women, 'madness' and exercise.

    Hardes, Jennifer Jane

    2018-03-21

    The positive relationship between exercise and mental health is often taken for granted in today's society, despite the lack of academic literature evidencing this symbiosis. Gender is considered a significant determinant in a number of mental health diagnoses. Indeed, women are considered twice as likely as men to experience the most pervasive mental health condition, depression. Exercise for women's mental health is promoted through various macrolevel charity, as well as microlevel, campaigns that influence government healthcare policy and National Health Service guidelines. Indeed, 'exercise prescriptions' in the treatment of depression is not uncommon. Yet, this link between exercise as a treatment for women's mental health has not always been so pervasive. In fact, an examination of asylum reports and medical journals from the late 19th century highlights a significant shift in attitude towards the role of exercise in the treatment of women's emotional states and mental health. This paper specifically examines how this treatment of women's mental health through exercise has moved from what might be regarded as a focus on exercise as a 'cause' of women's mental ailments to exercise promoted as a 'cure'. Unpacking the changing medical attitudes towards exercise for women in line with larger sociopolitical and historic contexts reveals that while this shift towards exercise promotion might prima facie appear as a less essentialist view of women and their mental and physical states, it inevitably remains tied to larger policy and governance agendas. New modes of exercise 'treatment' for women's mental health are not politically neutral and, thus, what appear to emerge as forms of liberation are, in actuality, subtler forms of regulation. © Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2018. All rights reserved. No commercial use is permitted unless otherwise expressly granted.

  1. Marijuana and madness

    Castle, David J; Murray, Robin, MD, M Phil, MRCP, MRC Psych; D'Souza, Deepak Cyril

    2012-01-01

    ...). It outlines the very latest developments in our understanding of the human cannabinoid system, and links this knowledge to clinical and epidemiological facts about the impact of cannabis on mental health...

  2. Madness, Dissidence and Transduction

    Craig Stewart Walker

    2017-01-01

    Full Text Available El artista disidente ruso Piotr (o Petr Pavlensky recibió atención internacional luego de que en 2013 se transmitiera un video en el que aparecía sentado con los testículos clavados a los adoquines de la Plaza Roja de Moscú. Posteriormente, se reveló que el incidente hacía parte de una serie de acciones artísticas de Pavlensky, que incluyeron coserse la boca, aparecer desnudo dentro de un rollo de alambre de púas frente a la sede de la Asamblea Legislativa, construir una barrera de neumáticos en llamas en el centro de San Petersburgo, haciendo alusión a los levantamientos políticos en Kiev, cortarse su propia oreja mientras se sentaba desnudo en la pared del Hospital Psiquiátrico de Serbsky y prender fuego a las puertas de madera del cuartel general del Servicio Secreto ruso. Aunque las autoridades rusas han intentado en varias ocasiones tratar a Pavlensky como si estuviera criminalmente loco, él sigue defendiendo de manera articulada sus actividades como obras de arte político disidente. Este artículo explora la idea de que Pavlensky usa deliberadamente la transducción como una herramienta por medio de la cual su trabajo cobra sentido. Pavlensky invoca conscientemente dos campos diferentes de interpretación cultural. La primera es la adhesión de las autoridades al Código Penal ruso, que se utiliza para ejercer un control despótico sobre el pueblo ruso. El segundo es el discurso internacional que rodea el arte de vanguardia, que defiende la libertad de expresión, incluidas las obras disidentes. Al asegurar que hay una transducción de su trabajo desde el primer campo al segundo, Pavlensky crea una colisión de opinión en la que la legitimidad de la política estatal de Rusia es desafiada y deslegitimada.

  3. Marijuana and madness

    Castle, David J; Murray, Robin, MD, M Phil, MRCP, MRC Psych; D'Souza, Deepak Cyril

    2012-01-01

    .... Clinically focused chapters review not only the direct psychomimetic properties of cannabis, but also the impact consumption has on the courses of evolving or established mental illnesses such as schizophrenia...

  4. Constitutive expression of the K-domain of a Vaccinium corymbosum SOC1-like (VcSOC1-K) MADS-box gene is sufficient to promote flowering in tobacco.

    Song, Guo-qing; Walworth, Aaron; Zhao, Dongyan; Hildebrandt, Britton; Leasia, Michael

    2013-11-01

    The K-domain of a blueberry-derived SOC1 -like gene promotes flowering in tobacco without negatively impacting yield, demonstrating potential for manipulation of flowering time in horticultural crops. The SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1) and SOC1-likes, belonging to the MIKC(c) (type II) MADS-box gene subfamily, are major floral activators and integrators of plant flowering. Both MADS-domains and K (Keratin)-domains are highly conserved in MIKC(c)-type MADS proteins. While there are many reports on overexpression of intact MIKC(c)-type MADS-box genes, few studies have been conducted to investigate the effects of the K-domains. In this report, a 474-bp K-domain of Vaccinium SOC1-like (VcSOC1-K) was cloned from the cDNA library of the northern highbush blueberry (Vaccinium corymbosum L.). Functional analysis of the VcSOC1-K was conducted by ectopically expressing of 35S:VcSOC1-K in tobacco. Reverse transcription PCR confirmed expression of the VcSOC1-K in T0 plants. Phenotypically, T1 transgenic plants (10 T1 plants/event) flowered sooner after seeding, and were shorter with fewer leaves at the time of flowering, than nontransgenic plants; but seed pod production of transgenic plants was not significantly affected. These results demonstrate that overexpression of the K-domain of a MIKC(c)-type MADS-box gene alone is sufficient to promote early flowering and more importantly without affecting seed production.

  5. Examination using LC-MS/MS determination of grayanotoxin levels in blood, urine, and honey consumed by patients presenting to the emergency department with mad honey intoxication and relations with clinical data: a preliminary study.

    Aygun, Ali; Gunduz, Abdulkadir; Turedi, Suleyman; Turkmen, Suha; Karaca, Yunus; Ayaz, Faik Ahmet; Ahn, Su Youn; Kim, Suncheun

    2015-01-01

    Intoxications related to "mad honey" are frequently encountered in the Black Sea region of Turkey. Intoxication is established on the basis of whether honey was consumed when history was taken at presentation. The search for a simple and reliable method for showing the grayanotoxins (GTXs) in mad honey in body fluids and in honey consumed by patients is still at the research stage. The purpose of this preliminary study was to investigate GTX levels in blood, urine, and honey consumed by patients with mad honey intoxication and to determine whether there is an association with clinical status. This descrptive study was conducted at the department of Emergency Medicine of Karadeniz Technical University Medical Faculty in Turkey. Mad honey, blood, and urine samples were obtained from patients between September 2013 and October 2014. Four cases presenting the Department of Emergency Medicine and diagnosed with mad honey intoxication were included in the study. GTX levels in blood, urine, and honey consumed by patients were determined using liquid chromatography-tandem mass spectrometry. Patients' mean blood GTX I level was 30.62 ng/mL, GTX III level 4.917 ng/mL, urine GTX I level 0.447 mg/mL, and GTX III level 1.998 mg/mL. The mean GTX I level in the honey samples consumed was 4.683 mg/g and GTX III level 8.423 mg/g. The present study is unique in representing the first time that GTXs have been determined in human body fluids. There is now an urgent need for a large series of studies to provide statistical evidence whether there is a relationship between levels of toxins in human body fluids and clinical picture.

  6. Neutral illusions, selective nightmares and autopoietic madness Ilusiones neutrales, pesadillas selectivas y locura autopoiética

    CARLOS Y VALENZUELA

    2010-12-01

    Full Text Available Until now, there are three evolutionary models: I The Synthetic Theory of Evolution (STE also named as the Neo-Darwinian model; II The Neutral Theory of Evolution (NTE with its daughter the Nearly-Neutral Theory of Evolution (NNTE; III The Theory of Evolution by means of Natural Drift (NDTE (Valenzuela 2007, 2009. All these theories accept that variation of genomes of living beings emerges by mutation. They disagree in relation of the causes of the acquisition and maintenance of the living being organization. STE proposes that the main factor for the acquisition and maintenance of fixated organization is selection and a marginal role of genetic drift. NTE proposed that the acquisition and maintenance of fixated organization is drift with a marginal role of selection, its daughter the NNTE includes besides selection with coefficients similar to the mutation rates. NDTE is derived from the conception of living beings as autopoietic processes, thus the acquisition and maintenance of organization occur by natural drift and living beings are always adapted. The extreme importance given to the factors of evolution, within each theory, has lead to absurd exaggerations that seems now illusions, nightmares or simply madness.Hasta el presente existen tres modelos evolutivos: I la Teoría Sintéti ca de la Evolución, (STE también conocida como modelo Neodarwiniano; II La Teoría Neutral de la Evolución (NTE con su hija la Teoría Casi-Neutral de la Evolución (NNTE; III La Teoría de la Evolución por Deriva Natural (NDTE. Todas las teorías aceptan que la variación genómica ocurre por mutación, pero discrepan en la importancia de los factores que llevan a la adquisición y mantención de la organización del ser vivo. La STE establece que el factor principal de la adquisición y mantención de los caracteres es la selección natural y concede un rol marginal a la deriva genética. La NTE establece que el factor principal de adquisición y mantenci

  7. Selvrefleksion som uddannelsesgreb – en kritisk diskussion

    Feilberg, Casper

    2015-01-01

    Hvordan kan ureflekteret motivation gribe forstyrrende ind i de studerendes videnskabelige arbejde, og hvordan kan man som vejleder forholde sig til disse private forhold på en både faglig og etisk forsvarlig måde? I artiklen vises eksempler på, at studerende nemt kan komme til at agere ud fra ur...

  8. ‘I am not very well I feel nearly mad when I think of you’: Male Jealousy, Murder and Broadmoor in Late-Victorian Britain

    Shepherd, Jade

    2017-01-01

    Abstract This article compares the representations of jealousy in popular culture, medical and legal literature, and in the trials and diagnoses of men who murdered or attempted to murder their wives or sweethearts before being found insane and committed into Broadmoor Criminal Lunatic Asylum between 1864 and 1900. It is shown that jealousy was entrenched in Victorian culture, but marginalised in medical and legal discourse and in the courtroom until the end of the period, and was seemingly cast aside at Broadmoor. As well as providing a detailed examination of varied representations of male jealousy in late-Victorian Britain, the article contributes to understandings of the emotional lives of the working-class, and the causes and representations of working-class male madness. PMID:29713114

  9. 'I am not very well I feel nearly mad when I think of you': Male Jealousy, Murder and Broadmoor in Late-Victorian Britain.

    Shepherd, Jade

    2017-05-01

    This article compares the representations of jealousy in popular culture, medical and legal literature, and in the trials and diagnoses of men who murdered or attempted to murder their wives or sweethearts before being found insane and committed into Broadmoor Criminal Lunatic Asylum between 1864 and 1900. It is shown that jealousy was entrenched in Victorian culture, but marginalised in medical and legal discourse and in the courtroom until the end of the period, and was seemingly cast aside at Broadmoor. As well as providing a detailed examination of varied representations of male jealousy in late-Victorian Britain, the article contributes to understandings of the emotional lives of the working-class, and the causes and representations of working-class male madness.

  10. Computing decay rates for new physics theories with FeynRules  and MadGraph 5 _aMC@NLO

    Alwall, Johan; Fuks, Benjamin; Mattelaer, Olivier; Öztürk, Deniz Gizem; Shen, Chia-Hsien

    2015-01-01

    We present new features of the FeynRules and MadGraph5/aMC@NLO programs for the automatic computation of decay widths that consistently include channels of arbitrary final-state multiplicity. The implementations are generic enough so that they can be used in the framework of any quantum field theory, possibly including higher-dimensional operators. We extend at the same time the conventions of the Universal FeynRules Output (or UFO) format to include decay tables and information on the total widths. We finally provide a set of representative examples of the usage of the new functions of the different codes in the framework of the Standard Model, the Higgs Effective Field Theory, the Strongly Interacting Light Higgs model and the Minimal Supersymmetric Standard Model and compare the results to available literature and programs for validation purposes.

  11. Electronic Signature (eSig)

    Department of Veterans Affairs — Beginning with the Government Paperwork Elimination Act of 1998 (GPEA), the Federal government has encouraged the use of electronic / digital signatures to enable...

  12. Islam reformerer sig selv indefra

    Provencal, Philippe

    2013-01-01

    En redegørelse for islams møde med moderniteten i det 19. og 20. århundrede. Der gøres rede for den islamiske reformismes og fundamentalismes historiske udvikling.......En redegørelse for islams møde med moderniteten i det 19. og 20. århundrede. Der gøres rede for den islamiske reformismes og fundamentalismes historiske udvikling....

  13. Relationer mellem familievirksomheder betaler sig

    Hollensen, Svend; Boyd, Britta

    2011-01-01

    This paper focuses on the international joint venture formation process of family businesses. The reason behind Danfoss’ decision to cooperate with two competing family businesses in Japan and China as well as two nonfamily businesses in Canada and Great Britain will be analysed. In-depth qualita...... the formation process, including competences and cultures. The study indicates which core competences of a family business matter when cooperating in equal split joint ventures. Implications for family business owners and ideas for future research are discussed.......This paper focuses on the international joint venture formation process of family businesses. The reason behind Danfoss’ decision to cooperate with two competing family businesses in Japan and China as well as two nonfamily businesses in Canada and Great Britain will be analysed. In...

  14. Análisis multicriterio SIG basado en momentos de orden superior normalizados para el cálculo de superficies de viabilidad ambiental

    Oscar Alberto Gómez Colorado

    2018-02-01

    Full Text Available El presente estudio plantea una metodología para generar áreas de criticidad y niveles de susceptibilidad ambiental aptas para el trazado o localización de proyectos de infraestructura, con un alto grado de certidumbre comparado con los métodos convencionales basados en las funciones de máximos, media aritmética y promedio ponderado. La metodología se basa en la superposición espacial de variables físicas, ambientales y socioeconómicas que miden el impacto en el territorio ante el paso o ubicación del proyecto de infraestructura; aplicando el método de análisis estadístico denominado “momentos de orden superior” con un factor exponencial de normalización, propuesto por los autores. El modelo de análisis propuesto aplica principalmente en el Diagnóstico Ambiental de Alternativas (DAA de proyectos lineales y concentrados, exigido por el Ministerio de Ambiente y Desarrollo Sostenible (MINAMBIENTE, en Colombia. El modelado espacial de los datos y las operaciones de superposición de capas de información, se realizaron a través de la herramienta Model Builder y la calculadora de mapas del software Arcgis. El análisis numérico y espacial realizado a través del SIG representa la interacción de los diferentes componentes bióticos, físicos y sociales (económicos, políticos y culturales, que inciden en la viabilidad del trazado o ubicación de un proyecto de infraestructura (vías, oleoductos, líneas de transmisión de energía, centrales hidroeléctricas, rellenos sanitarios, entre otros. Los resultados obtenidos demuestran como la superficie de susceptibilidad general, calculada con el método de momentos de orden superior normalizado, permite una toma de decisiones adecuada, toda vez que puede ajustarse con mayor aproximación a las diferentes restricciones ambientales de la región.

  15. Metodologia para a monitorização e gestão da pesca de arrasto em Portugal. O exemplo do SIG GEOCRUST 1.0

    Joana Simões

    2003-12-01

    Full Text Available A METHODOLOGY FOR THE MONITORING AND MANAGEMENT OF TRAWL FISHERIES IN PORTUGAL. THE SIG EXAMPLE OF GEOCRUST 1.0. This paper aims to present the Geographical Information System GEOCRUST 1.0, a computer programme developed within the fishery research project DGXIV 99/059, Use of Satellite GPS data to map effort and landings of the Portuguese crustacean fleet (GEOCRUST, in the University of Algarve. This stand-alone application was developed essentially to map fishing effort and catch per unit effort (CPUE information for the Portuguese crustacean trawl fleet, operating off the Southwest and South coasts of Portugal. The data available for this study included GPS vessels’ geographical positions and speed, transmitted via satellite to the Portuguese fisheries inspection authorities, and their catch reported to the Portuguese Directorate-General of Fisheries. GEOCRUST 1.0 was developed in ®VISUAL BASIC 6.0 using two ActiveX control: ADODB, to connect with the ®MSACCESS 2000 database and ®MAPOBJECTS 2.0 PRO, to communicate with the maps and provide GIS facilities. The application includes seven modules with the following objectives: Module 1 – to map and edit the original VMS data for a single vessel or group of vessels, for different periods of time; Module 2 – to analyse the trawl speed of each vessel; Module 3 – to define the boundaries of all fishing trips performed by vessel along the year; Module 4 – to identify and define the trawl hauls, within each fishing trip; Module 5 – to issue maps of fishing effort and CPUE; Module 6 – for exploratory data analysis and to extract data from the DB for further use in other computer packages and Module 7 – to recreate the activity of a single vessel, group of vessels or the total fleet, for a fixed period of time. This GIS application, fully developed by programming, has the advantage of being able to be used on its own without any need for external software support. The system developed

  16. VEGETACIÓN DEL PARQUE COPAHUE: APLICACIÓN DE UNA METODOLOGÍA BIOCLIMÁTICA MEDIANTE EL USO DE INFORMACIÓN SATELITAL Y SIG

    Oscar Peña

    2003-12-01

    Full Text Available La distribución de la vegetación natural se encuentra en intima relación con el comportamiento de los elementos climáticos tal como la temperatura y la humedad. Asimismo la altitud, la orientación (exposición a la radiación y la pendiente modifican dicho comportamiento. El Parque Provincial Copahue, localizado en la Provincia de Neuquen, en la cordillera norpatagónica, presenta características biogeográficas y particularidades ecogeomorfológicas que evidencian el marcado efecto de las variables climáticas. El presente trabajo tiene por objetivo analizar las relaciones de dichas variables mediante la utilización de una metodología bioclimática y el tratamiento digital de imágenes Landsat Tm. Metodológicamente se procedió en tres pasos. El primero consistió en la clasificación supervisada de la imagen satelital. De esto surgió una carta de vegetación del parque. En el segundo paso se digitalizó cartografía 1:100.000 con la que se obtuvo un modelo digital de elevación, mapa de pendiente y de orientación de laderas, de temperaturas y precipitaciones. En el tercero, se confeccionó una base de datos para la aplicación de una metodología bioclimática (Rivas y Martínez.1994 que permitió, mediante la aplicación del SIG delimitar los bioclimas del parque. La información de vegetación obtenida mediante la clasificación digital de la imagen satelital fue cruzada con el mapa bioclimático, el mapa topográfico, de pendiente, orientación y de las variables climáticas consideradas, a fin de conocer el grado de interrelación entre la distribución de la vegetación con las variables físicas tratadas.

  17. SIG E REGRESSÃO LINEAR PARA AVALIAÇÃO AMBIENTAL DAS NASCENTES DO RIO SUBAÉ EM FEIRA DE SANTANA-BA

    Elinaldo Sacramento dos Santos

    2013-08-01

    Full Text Available Na bacia hidrográfica do rio Subaé, as cabeceiras e as lagoas são destinadas a vários fins (usos urbano, agrícolae industrial, os quais podem comprometer a qualidade destes ambientes. Para avaliar a qualidade das nascentesdo rio Subaé, optou-se pelo monitoramento dos parâmetros físicos, químicos e microbiológicos realizados emquatorze pontos de observação da qualidade da água, e pelo mapeamento dos aspectos sociais e ambientaispresentes no meio. Para o alcance desse objetivo, em conjunto com a pesquisa, foi necessário também o uso detécnicas de análise espacial no ambiente SIG e a geoestatística (regressão linear. Os resultados da qualidadeda água – principalmente os teores de OD (oxigênio dissolvido e DBO (demanda bioquímica de oxigênio, ea turbidez – foram comparados aos níveis e parâmetros estabelecidos pela Resolução do Conselho Nacionalde Meio Ambiente (CONAMA nº 357/05. Esses resultados refletiram o estado ambiental de alguns pontosdas nascentes, que apresentaram parâmetros adversos aos estabelecidos pela legislação brasileira – comofoi o caso do OD e da DBO nos pontos da nascente do rio Subaé, na rua Pedro Suzart (domínio de efluentesdomésticos, e turbidez em pontos das Lagoas Subaé e Salgada. Para se identificar a influência de um dosparâmetros de qualidade de água e a variabilidade dos aspectos sociais e ambientais (correlações entre osdados de OD e as variáveis ambientais empregou-se a regressão linear. Essa análise permitiu concluir queas variáveis sociais e ambientais, altimetria, inclinação do terreno e densidade populacional são os fatoresdeterminantes nos valores de OD nos ambientes estudados.

  18. Use of Elements of the Stewart Model (Strong Ion Approach) - SID3, SID4, Atot/A-, SIDe and SIG for the Diagnostics of Respiratory Acidosis in Brachycephalic Dogs.

    Sławuta, P; Sapikowski, G; Sobieraj, B

    2016-09-01

    Buffer systems of blood and tissues, which have the ability to bind with and give up hydrogen ions, participate in maintaining the acid-base balance (ABB) of the organism. According to the classic model, the system of carbonic acid and bicarbonates, where the first component serves the role of an acid and the second a base, determines plasma pH. The so-called Stewart model, which assumes that ions in blood serum can be separated into completely dissociated - nonbuffer and not dissociated - buffer ions which may give up or accept H+ions, also describes the ABB of the organism. The goal of the study was to find out whether, during respiratory acidosis, the values of SID3, SID4, Atot/A-, SIDe and SIG change. The study was carried out on 60 adult dogs of the boxer breed (32 males and 28 females) in which, on the basis of an arterial blood test, respiratory acidosis was found. A strong overgrowth of the soft palate tissue requiring a surgical correction was the cause of the ABB disorder. Prior to surgery and on the 14th day after the surgery, venous and arterial blood was drawn from each dog. ABB parameters were determined in the arterial blood sample: the blood pH, pCO2 and HCO3-. In the venous blood, concentration of Na+, K+, Cl-, lactate-, albumins, and Pinorganic was determined. On the basis of the obtained data, the values of SID3, SID4, SIDe, A- and SIG, before and after the surgery, were calculated. In spite of the fact that the average concentration of ions, albumins, Pinorganic and lactate in the blood serum of dogs before and after the surgical procedure was similar and within the physiological norms, the values of SID3, SIDe and SIG, calculated on the basis of the former, displayed statistically significant differences. On the basis of the results obtained, it can be stated that the values of SID3, SIDe and SIG change during respiratory acidosis and may be helpful in the diagnostics of ABB disorders in brachycephalic dogs.

  19. Tilværelsen som gymnasieelev: en undersøgelse af, hvorvidt nutidens gymnasieelever føler sig stressede og pressede i deres gymnasietilværelse

    Hansen, Louise Bach

    2017-01-01

    I specialeafhandlingen har jeg undersøgt tilværelsen som gymnasieelev i nutidens Danmark, samt hvorvidt eleverne føler sig henholdsvis stressede og pressede i gymnasietilværelsen. Flere kvantitative undersøgelser (#5 DGS 2013-2014, Bendtsen et al. 2014) omkring elevers trivsel i gymnasieskolen viser, at over halvdelen af de adspurgte elever i undersøgelserne tilkendegiver, at de er stressede eller pressede dagligt eller ofte i tilværelsen. Men hvad ligger til grund for elevernes svar? Er de v...

  20. Localização de instalações com o auxílio de Sistema de Informações Geográficas (SIG) e modelagem matemática

    Mapa, Silvia Maria Santana; Lima, Renato da Silva; Mendes, José F. G.

    2006-01-01

    Problemas de localização de instalações envolvem escolher o melhor local para uma ou mais instalações dentro de um conjunto de locais possíveis, sendo uma questão logística de ampla abrangência e de grande importância. Uma das ferramentas que auxiliam na resolução desses problemas são os Sistemas de Informações Geográficas (SIG), pela sua grande capacidade em armazenar, exibir e manipular dados espacialmente distribuídos. Essa capacidade aumenta consideravelmente quando se faz seu us...

  1. Los SIG como herramienta para la “geoprevención”: análisis de los patrones espaciales de los delitos contra el patrimonio en el municipio de Santander (2008-2013)

    Montero Arabia, Laura

    2014-01-01

    Resumen: La prevención del delito ha tomado mayor importancia en las últimas décadas. Se busca anticiparse al delincuente y no esperar a que el hecho delictivo se produzca. Este trabajo es un ensayo metodológico aplicado de cómo las herramientas SIG pueden ayudar a la geoprevención de una tipología delictiva concreta en un ámbito de estudio determinado, en este caso el municipio de Santander. Abstract: The prevention of crime has become more relevant in recent decades, It is looked for the...

  2. Estudio y análisis de la calidad de la educación secundaria en España desde una perspectiva geomática con herramientas de cartografía y SIG.

    Aragón Velasco, Aurelio

    2015-01-01

    En el presente Trabajo Fin de Grado se realiza un estudio y análisis geomático de la calidad de la educación secundaria mediante procedimientos de Cartografía y SIG, con datos espaciales y temáticos de fuentes oficiales que garantizan la precisión y certeza necesaria para la obtención de unas conclusiones objetivas y de calidad. Dicha calidad de la educación secundaria será estudiada bajo el marco de referencia de los estudios dek informe PISA (por sus siglas en inglés: Programme for Inter...

  3. APLICACIÓN DE LOS SIG PARA LA EVALUACIÓN DEL ESTADO DE CONSERVACIÓN DEL HÁBITAT DEL PAUJIL DE PICO AZUL CRAX ALBERTI (AVES: CRACIDAE EN EL NORORIENTE DE ANTIOQUIA, COLOMBIA

    Adriana María Molina

    Full Text Available El paujil de pico azul, Crax alberti, es un ave endémica del norte de Colombia en peligro crítico de extinción, sometida a la pérdida del hábitat y a la cacería de subsistencia como principales amenazas. Se desarrolló una aplicación de Sistemas de Información Geográfica (SIG para comparar el estado de conservación de las áreas de bosque, hábitat del paujil de pico azul, en 15 municipios de la región del nororiente de Antioquia en dos períodos de tiempo diferentes (1986 y 2002. La aplicación desarrollada que incorpora información cartográfica digital permitió clasificar ocho zonas definidas en el área de estudio para realizar un análisis de las prioridades de conservación. Se formularon, además, algunas recomendaciones para futuras aplicaciones de SIG como herramienta para la gestión de la conservación de especies amenazadas en Colombia.

  4. The DAL10 gene from Norway spruce (Picea abies) belongs to a potentially gymnosperm-specific subclass of MADS-box genes and is specifically active in seed cones and pollen cones.

    Carlsbecker, Annelie; Sundström, Jens; Tandre, Karolina; Englund, Marie; Kvarnheden, Anders; Johanson, Urban; Engström, Peter

    2003-01-01

    Transcription factors encoded by different members of the MADS-box gene family have evolved central roles in the regulation of reproductive organ development in the flowering plants, the angiosperms. Development of the stamens and carpels, the pollen- and seed-bearing organs, involves the B- and C-organ-identity MADS-box genes. B- and C-type gene orthologs with activities specifically in developing pollen- and seed-bearing organs are also present in the distantly related gymnosperms: the conifers and the gnetophytes. We now report on the characterization of DAL10, a novel MADS-box gene from the conifer Norway spruce, which unlike the B- and C-type conifer genes shows no distinct orthology relationship to any angiosperm gene or clade in phylogenetic analyses. Like the B- and C-type genes, it is active specifically in developing pollen cones and seed cones. In situ RNA localization experiments show DAL10 to be expressed in the cone axis, which carry the microsporophylls of the young pollen cone. In contrast, in the seed cone it is expressed both in the cone axis and in the bracts, which subtend the ovuliferous scales. Expression data and the phenotype of transgenic Arabidopsis plants expressing DAL10 suggest that the gene may act upstream to or in concert with the B- and C-type genes in the establishment of reproductive identity of developing cones.

  5. Reply to Mas et al.: Comment on Gebhardt et al. MAD-MEX: Automatic Wall-to-Wall Land Cover Monitoring for the Mexican REDD-MRV Program Using All Landsat Data. Remote Sens. 2014, 6, 3923–3943

    Michael Schmidt

    2016-06-01

    Full Text Available Mas, J.F. et al. have submitted a paper [1] for publication, which aims to respond to a paper published by Gebhardt et al. [2]. Mas, J.F. et al. had received a consultancy in 2013 to assess the quality of the early prototype products partly described in Gebhardt et al. in 2014. This consultancy, although a formal non-disclosure agreement had not been demanded, was awarded under the mutual understanding that the data handed over to Mas et al. constitute the early development phase of the program. Therefore, Mas et al. had been asked to give an assessment on the quality of the prototypes to obtain a proof of concept for the proposed workflow of MAD-Mex. It was clear that this assessment would suffer from limited availability of high quality training and validation data available in 2013. Mas et al. finally did not execute the consultancy due to the limited vector processing capacities in their lab. In October 2014, we sent the latest products, version 4.2 of the MAD-Mex products, including the more than 200,000 validation points gathered from independent expert interpreters of all Mexican ecosystems. Mas et al. did not respond to this transfer or to our request to collaborate in the quality control and assessment of MAD-Mex.

  6. “I’m in the Hospice, god”: problematizations about the madness, the hospice and the psychiatry in the diary of Maura Lopes Cançado (Brazil, 1959-60

    Yonissa Wadi

    2017-12-01

    Full Text Available The writer Maura Lopes Cançado circulated in the world of the psychiatric hospitals between the 1950s, 1960s and 1970s. During one of hospitalizations (1959-1960, the third time in the National Psychiatric Center, a hospital complex in Rio de Janeiro, the writer wrote a diary that was later published as the book Hospice is God-Diary I. The bond between the live lived by her and the fiction, which the narrator transited in her diary, creating a unique work from the perspective of academic commentators and literary critics. In the history field of madness and psychiatry, this work offers new possibilities for understanding the configuration of psychiatric care, scientific and therapeutic practices and the various subjects that circulated in the world of Brazilians psychiatric hospitals, in the 1950s, operating a displacement in relation to traditional places of enunciation that are known. In this article, I chose to observe the problematizations of Maura about the institutional daily life and the fact of writing a diary, which oscillate between teaching others and the care of the self. Therefore, I did an enunciative analysis of the narrative, that values the things that were said by her as one of the truths about the psychiatric hospital, medical science and its practices, the mad and the madness.

  7. Aḥmad Muṣṭafā Ṭāshkubrīzāde’s (d. 968/1561 polemical tract against Judaism

    Schmidtke, Sabine

    2008-06-01

    Full Text Available This article deals with a short polemical tract against Judaism written by the well-known Ottoman scholar Aḥmad Muṣṭafā Ṭāshkubrīzāde (d. 968/1560. The author uses the same arguments known to us from medieval polemics, viz. that the Torah, which was abrogated by Islam, contains references to the Prophet Muḥammad, despite the fact that it was tampered with by the Jews. In addition to the Bible, Ṭāshkubrīzāde quotes a number of later Jewish sources that add an important dimension to his tract. After a brief introduction in which the possibility of the author's indebtedness to Ibn Abī ‘Abd al-Dayyān, a Jewish convert to Islam, is discussed, an edition and translation of the text are provided.

    Este artículo estudia un corto tratado de polémica en contra del judaísmo escrito por el conocido erudito otomano Aḥmad Muṣṭafā Ṭāšhkubrīzāde (m. 968/1560. El autor utiliza los mismos argumentos que conocemos de la polémica religiosa medieval, tales como que la Torah, abrogada por el Islam, contenía referencias al Profeta Muḥammad a pesar de que su texto fue corrompido por los judíos. Además de la Biblia, Ṭāšhkubrīzāde cita una serie de fuentes judías tardías que añaden una importante dimensión a este trabajo. Después de una breve introducción en que se discute la posibilidad de la deuda del autor respecto a la obra de Ibn Abī ‘Abd al-Dayyān, un converso del judaísmo al Islam, se presenta una edición y traducción del texto.

  8. The regulatory mechanism of fruit ripening revealed by analyses of direct targets of the tomato MADS-box transcription factor RIPENING INHIBITOR

    Fujisawa, Masaki; Ito, Yasuhiro

    2013-01-01

    The developmental process of ripening is unique to fleshy fruits and a key factor in fruit quality. The tomato (Solanum lycopersicum) MADS-box transcription factor RIPENING INHIBITOR (RIN), one of the earliest-acting ripening regulators, is required for broad aspects of ripening, including ethylene-dependent and -independent pathways. However, our knowledge of direct RIN target genes has been limited, considering the broad effects of RIN on ripening. In a recent work published in The Plant Cell, we identified 241 direct RIN target genes by chromatin immunoprecipitation coupled with DNA microarray (ChIP-chip) and transcriptome analysis. Functional classification of the targets revealed that RIN participates in the regulation of many biological processes including well-known ripening processes such as climacteric ethylene production and lycopene accumulation. In addition, we found that ethylene is required for the full expression of RIN and several RIN-targeting transcription factor genes at the ripening stage. Here, based on our recently published findings and additional data, we discuss the ripening processes regulated by RIN and the interplay between RIN and ethylene. PMID:23518588

  9. Functional and evolutionary analysis of the AP1/SEP/AGL6 superclade of MADS-box genes in the basal eudicot Epimedium sagittatum.

    Sun, Wei; Huang, Wenjun; Li, Zhineng; Song, Chi; Liu, Di; Liu, Yongliang; Hayward, Alice; Liu, Yifei; Huang, Hongwen; Wang, Ying

    2014-03-01

    MADS-box transcriptional regulators play important roles during plant development. Based on phylogenetic reconstruction, the AP1/SEP/AGL6 superclade of floral MADS-box genes underwent one or two duplication events in the common ancestor of the core eudicots. However, the functional evolution of the AP1/SEP/AGL6 superclade in basal eudicots remains uncharacterized. Epimedium sagittatum is a basal eudicot species valued for its medicinal properties and showing unique floral morphology. In this study, structural and functional variation of FUL-like (AP1 subfamily), SEP-like and AGL6-like genes in this species was investigated to further our understanding of flower evolution in angiosperms. Detailed investigations into the microsynteny and evolutionary history of the floral A and E class MADS-box genes in eudicots were undertaken and used to trace their genomic rearrangements. One AP1-like gene, two SEP-like genes and one AGL6-like gene were cloned from E. sagittatum. Their expression patterns were examined using quantitative RT-PCR in different vegetative and reproductive organs at two developmental stages. Yeast two-hybrid assays were carried out among AP1/SEP/AGL6 superclade, AP3/PI and AGAMOUS subfamily members for elucidation of dimerization patterns. In addition, possible formation of a ternary complex involving B class proteins with the A class protein EsFUL-like, the E class SEP-like protein EsAGL2-1 or the AGL6-class protein EsAGL6 were detected using yeast three-hybrid assays. Transgenic Arabidopsis or tobacco plants expressing EsFUL-like, EsAGL2-1 and EsAGL6-like under the cauliflower mosaic virus (CaMV) 35S promoter were generated and analysed. Genomic studies of AP1 syntenic regions in arabidopsis, columbine, strawberry, papaya, peach, grapevine and tomato were conducted for microsyntenic analyses. Sequence and phylogenetic analyses showed that EsFUL-like is a member of the AP1 (A class) subfamily, EsAGL2-1 and EsAGL2-2 belong to the SEP-like (E class

  10. Aplicación de las técnicas de teledetección y SIG a la determinación y análisis de mapas de evapotranspiración

    Reca Cardeña, Juan; Medina, J. L.; Camacho, E. F.; López, R.; Roldán Cañas, José

    1999-01-01

    La estimación de la evapotranspiración es esencial en los sistemas de riego, en el cálculo de pérdidas de agua en embalses, en la estimación de balances hídricos, en las predicciones de escorrentía, así como en estudios climatológicos y meteorológicos. Las nuevas técnicas denominadas teledetección y sistemas de información geográfica (SIG) permiten el cálculo de la distribución espacial de este parámetro. Un nuevo modelo racional, SEBAL (Bastiaanssen, 1995), basado en la ecuación de conservac...

  11. APLIKASI SIG UNTUK PEMETAAN INDEKS KEPEKAAN LINGKUNGAN: STUDI KASUS DI PESISIR CILACAP DAN SEGARA ANAKAN (GIS Application for Environmental Sensitivity Index Mapping Case Study in Cilacap Coastal Area and Segara Anakan

    Utantyo Utantyo

    2015-01-01

    Full Text Available ABSTRAK Sumberdaya pesisir dapat menerima dampak dari kecelakaan tumpahan minyak. Polusi minyak dapat terjadi dalam berbagai situasi lingkungan. Dalam hal ini, inventarisasi pesisir secara detil dikombinasikan dengan indeks sensitivitas memungkinkan ketersediaan informasi pada tingkat yang lebih baik bagi perencana pengelolaan tumpahan minyak. Sistem Informasi Geografis (SIG=Geographic Information System dapat meningkatkan penggunaan data yang dibutuhkan dalam menanggapi adanya tumpahan minyak tersebut serta perencanaan darurat. Studi ini dilakukan untuk mempelajari sensitivitas lingkungan dan mengkombinasikannya untuk membentuk prototipe sistem informasi sensitivitas lingkungan. Dengan menggunakan SIG di daerah pesisir Segara Anakan dan Cilacap. Salah satu strategi yang penting di dalam perencanaan darurat adanya tumpahan minyak adalah memprioritaskan respons tumpahan. Lingkungan pesisir dapat dikuantitatifkan dengan menetapkan skema klasifikasi indeks sensitivitas lingkungan (ISE=Environmemntal Sensitivity Index: ESI. Sensitivitas lingkungan mencerminkan derajad reaksi dari wilayah pesisir untuk bertatran dan pulih ketika terjadi bencana tumpahan minyak. Metode untuk menetapkan ISE adalah dengan mengkombinasikan factor-faktor yang terkait dari suatu sensitivitas lingkungan, antara lain (a paparan terhadap energi gelombang dan pasut, (b pelerengan garis pantai, (c jenis substrata, dan (d produktivitas biologi. Studi ini menekankan kemampuan SIG untuk memvisualisasikan dan memodernkan faktor-faktor sensitivitas lingkungan secara spasial. Hasil penelitian menunjukkan bahwa aplikasi SIG untuk mengelola memanipulasi dan menayangkan data pesisir yang relevan dapat dilaksanakan dan dapat dicatat keunggulannya di dalam upaya pemetaan sensitivitas lingkungan. Dalam penelitian ini wilayah pesisir Cilacap memiliki sensitivitas medium, karena daerah tersebut dibatasi oleh garis pantai ESI 5 (dengan panjang 11,6 km, dan dengan ESI 6 Sungai Donan (12,3 km

  12. GIS for diagnosis and environmental monitoring of degraded area by shale mining in Sao Mateus do Sul - Parana state, Brazil; SIG (sistemas de informacoes geograficas) para diagnostico e monitoramento ambiental da area degradada pela mineracao de xisto em Sao Mateus do Sul - PR

    Freitas, Cinthia Obladen de Almendra [Pontificia Univ. Catolica do Parana, Curitiba, PR (Brazil). Inst. de Saneamento Ambiental; Brandalize, Maria Cecilia Bonato [Pontificia Univ. Catolica do Parana, Curitiba, PR (Brazil). Dept. de Arquitetura e Urbanismo]. E-mail: cinthia@rla01.pucpr.br

    1997-07-01

    This work present a geographical information system (GIS) used for diagnosis and monitoring of Corrego Cachoeira hydrographic basin. The basin will be provided with a digital cartography base and alphanumeric data base, allowing the integration and development of various other studies. The structure of SIG, as well as software and hardware used by the project are presented.

  13. Análisis de la metodología de los Sistemas de Información Geográfica (SIG en la cartografía de la guerra en Colombia

    Nicolás Espinosa

    2013-01-01

    Full Text Available En el artículo presentamos la metodología que utilizamos en la construcción de un Atlas sobre el conflicto armado en Antioquia. En particular, nos enfocamos en el análisis de la construcción de mapas cuantitativos por medio de Sistemas de Información Geográfica. En el artículo destacamos que si bien hay un incipiente debate académico en torno a la naturaleza de las bases de datos sobre el Conflicto Armado, la discusión sobre la utilidad, alcances y limitaciones de las metodologías cartográficas al respecto es casi inexistente. En la medida que los mapas no son neutros, pues representan los espacios tanto como pueden ser capaces de crearlos, como aporte a los estudios críticos desde los SIG, nos detendremos a develar y explorar tres aspectos que subyacen la creación de mapas sobre la guerra y que por lo general permanecen ocultos. Son pues elementos analíticos que podrán ser útiles tanto para quien lea un mapa como para quien lo produzca: (i las consecuencias a que dan lugar el uso de distintas bases cuantitativas, (ii el papel que juegan las formas de clasificación estadística de los datos y (iii las múltiples formas de representación cartográfica que los SIG ofrecen. Los mapas compendiados en el Atlas, tal y como exponemos en el artículo, permiten apreciar los múltiples resultados analíticos a que dan lugar la covariación de estos aspectos (fuentes, clasificaciones y representaciones a la hora de construir y leer un mapa.

  14. Swift J1644+57 gone MAD: the case for dynamically important magnetic flux threading the black hole in a jetted tidal disruption event

    Tchekhovskoy, Alexander; Metzger, Brian D.; Giannios, Dimitrios; Kelley, Luke Z.

    2014-01-01

    The unusual transient Swift J1644+57 likely resulted from a collimated relativistic jet, powered by the sudden onset of accretion on to a massive black hole (BH) following the tidal disruption (TD) of a star. However, several mysteries cloud the interpretation of this event, including (1) the extreme flaring and `plateau' shape of the X-ray/γ-ray light curve during the first t - ttrig ˜ 10 d after the γ-ray trigger; (2) unexpected rebrightening of the forward shock radio emission at t - ttrig ˜ months; (3) lack of obvious evidence for jet precession, despite the misalignment typically expected between the angular momentum of the accretion disc and BH; (4) recent abrupt shut-off in the jet X-ray emission at t - ttrig ˜ 1.5 yr. Here, we show that all of these seemingly disparate mysteries are naturally resolved by one assumption: the presence of strong magnetic flux Φ• threading the BH. Just after the TD event, Φ• is dynamically weak relative to the high rate of fall-back accretion dot{M}, such that the accretion disc (jet) freely precesses about the BH axis = our line of sight. As dot{M} decreases, however, Φ• becomes dynamically important, leading to a state of `magnetically arrested disk' (MAD). MAD naturally aligns the jet with the BH spin, but only after an extended phase of violent rearrangement (jet wobbling), which in Swift J1644+57 starts a few days before the γ-ray trigger and explains the erratic early light curve. Indeed, the entire X-ray light curve can be fitted to the predicted power-law decay dot{M} ∝ t^{-α } (α ≃ 5/3 - 2.2) if the TD occurred a few weeks prior to the γ-ray trigger. Jet energy directed away from the line of sight, either prior to the trigger or during the jet alignment process, eventually manifests as the observed radio rebrightening, similar to an off-axis (orphan) γ-ray burst afterglow. As suggested recently, the late X-ray shut-off occurs when the disc transitions to a geometrically thin (jetless) state once

  15. Foucault, Derrida e a História da Loucura: notas sobre uma polêmica Foucault, Derrida, and the history of madness: notes on a controversy

    André de Faria Pereira Neto

    1998-07-01

    Full Text Available A publicação do livro Folie et Déraison. Histoire de la Folie à l'âge Classique (1961, de Michel Foucault, promoveu um debate entre o autor e o filósofo Jacques Derrida, durante os anos 60/70. Derrida criticou a proposta metodológica e a organização da História da Loucura, apresentadas por Foucault, no prefácio da primeira edição. Essa polêmica parece ter motivado o autor a retirar, da segunda edição, o prefácio que abria a primeira versão do livro. O objetivo deste artigo é analisar alguns pontos presentes nesta controvérsia. Além disso, ele apresenta uma agenda de pesquisa para o entendimento das razões que levaram Foucault a tomar tal atitude.The publication of the book Folie et Déraison. Histoire de la Folie à l'âge Classique (1961, by Michel Foucault, sparked a debate between the author and philosopher Jacques Derrida during the 1960s and 70s. Derrida criticized the methodological proposal and organization of the History of Madness presented by Foucault in the foreword to the first edition. The controversy appears to have motivated the author to withdraw this same foreword from the second edition. The purpose of this article is to analyze some current points in this controversy. It also presents a research agenda for an understanding of the reasons leading Foucault to take this stance.

  16. Digital gene expression analysis of male and female bud transition in Metasequoia reveals high activity of MADS-box transcription factors and hormone-mediated sugar pathways.

    Zhao, Ying; Liang, Haiying; Li, Lan; Tang, Sha; Han, Xiao; Wang, Congpeng; Xia, Xinli; Yin, Weilun

    2015-01-01

    Metasequoia glyptostroboides is a famous redwood tree of ecological and economic importance, and requires more than 20 years of juvenile-to-adult transition before producing female and male cones. Previously, we induced reproductive buds using a hormone solution in juvenile Metasequoia trees as young as 5-to-7 years old. In the current study, hormone-treated shoots found in female and male buds were used to identify candidate genes involved in reproductive bud transition in Metasequoia. Samples from hormone-treated cone reproductive shoots and naturally occurring non-cone setting shoots were analyzed using 24 digital gene expression (DGE) tag profiles using Illumina, generating a total of 69,520 putative transcripts. Next, 32 differentially and specifically expressed transcripts were determined using quantitative real-time polymerase chain reaction, including the upregulation of MADS-box transcription factors involved in male bud transition and flowering time control proteins involved in female bud transition. These differentially expressed transcripts were associated with 243 KEGG pathways. Among the significantly changed pathways, sugar pathways were mediated by hormone signals during the vegetative-to-reproductive phase transition, including glycolysis/gluconeogenesis and sucrose and starch metabolism pathways. Key enzymes were identified in these pathways, including alcohol dehydrogenase (NAD) and glutathione dehydrogenase for the glycolysis/gluconeogenesis pathway, and glucanphosphorylase for sucrose and starch metabolism pathways. Our results increase our understanding of the reproductive bud transition in gymnosperms. In addition, these studies on hormone-mediated sugar pathways increase our understanding of the relationship between sugar and hormone signaling during female and male bud initiation in Metasequoia.

  17. Digital gene expression analysis of male and female bud transition in Metasequoia reveals high activity of MADS-box transcription factors and hormone-mediated sugar pathways

    Ying eZhao

    2015-06-01

    Full Text Available Metasequoiaglyptostroboidies is a famous redwood tree of ecological and economic importance, and requires more than 20 years of juvenile-to-adult transition before producing female and male cones. Previously, we induced reproductive buds using a hormone solution in juvenile Metasequoia trees as young as5-to-7years old. In the current study, hormone-treated shoots found in female and male buds were used to identify candidate genes involved in reproductive bud transition in Metasequoia. Samples from hormone-treated cone reproductive shoots and naturally occurring non-cone setting shoots were analyzed using 24 digital gene expression (DGE tag profiles using Illumina, generating a total of 69,520 putative transcripts. Next, 32 differentially and specifically expressed transcripts were determined using quantitative real-time polymerase chain reaction, including the upregulation of MADS-box transcription factors involved in male bud transition and flowering time control proteins involved in female bud transition. These differentially expressed transcripts were associated with 243 KEGG pathways. Among the significantly changed pathways, sugar pathways were mediated by hormone signals during the vegetative-to-reproductive phase transition, including glycolysis/gluconeogenesis and sucrose and starch metabolism pathways. Key enzymes were identified in these pathways, including alcohol dehydrogenase (NAD and glutathione dehydrogenase for the glycolysis/gluconeogenesis pathway, and glucanphosphorylase for sucrose and starch metabolism pathways. Our results increase our understanding of the reproductive bud transition in gymnosperms. In addition, these studies on hormone-mediated sugar pathways increase our understanding of the relationship between sugar and hormone signaling during female and male bud initiation in Metasequoia.

  18. Motherhood and the "Madness of Hunger": "...Want Almal Vra vir My vir 'n Stukkie Brood" ("...Because Everyone Asks Me for a Little Piece of Bread").

    Kruger, Lou-Marié; Lourens, Marleen

    2016-03-01

    children can be thought of as evoking a kind of "madness" in low-income mothers.

  19. 3 dimensional distributions of NO2, CHOCHO, and HCHO measured by the University of Colorado 2D-MAX-DOAS during MAD-CAT

    Ortega, Ivan; Sinreich, Roman; Volkamer, Rainer

    2014-05-01

    We present results of 2 dimensional Multi Axis-DOAS (2D-MAX-DOAS) measurements to infer 3-dimensional measurements of trace gases by characterizing boundary layer vertical profiles and near surface azimuth horizontal distribution of NO2 (14 angles covering 360°). We combine the established optimal estimation inversion with a new parameterization approach; the first method to derive NO2 tropospheric vertical profiles and boundary layer height and the second one to retrieve the azimuth horizontal distribution of near surface NO2 mixing ratios, both at multiple wavelengths (350 nm, 450 nm, and 560 nm). This was conducted for three cloud-free days in the framework of the intensive Multi Axis DOAS Comparison campaign for Aerosols and Trace gases (MAD-CAT) in Mainz, Germany 2013. By retrieving NO2 at multiple wavelengths range-resolved distributions of NO2 are derived using an 'Onion-peeling' approach, i.e., exploiting the fact that the optical path lengths at different wavelengths probe different horizontal air masses. We also measure glyoxal (CHOCHO) and formaldehyde (HCHO) distributions, and present to our knowledge the first 3-dimesional trace-gas distribution measurements of CHOCHO by a ground-based instrument. We expand the 2D-MAX-DOAS capabilities to calculate azimuth ratios of HCHO-to-NO2 (RFN) and CHOCHO-to-NO2 (RGN) to pinpoint volatile organic compound (VOC) oxidation chemistry and CHOCHO-to-HCHO (RGF) ratios as an indicator of biogenic and/or anthropogenic VOC emissions. The results of RFN correlate well with RGN and we identify azimuth variations that indicate gradients in the VOC/NOx chemistry that leads to O3 and secondary aerosol production. While there is a clear diurnal pattern in the RFN and RGN, no such variations are observed in the RGF, which shows rather constant values below 0.04 throughout the day, consistent with previous measurements, and indicative of urban air masses.

  20. Variations on a theme in fruit development: the PLE lineage of MADS-box genes in tomato (TAGL1) and other species.

    Garceau, Danielle C; Batson, Megan K; Pan, Irvin L

    2017-08-01

    This article focuses on the role of TOMATO AGAMOUS-LIKE 1 (TAGL1) on a wide range of ripening functions in tomato. We also examine orthologs of this gene in related species that produce different fruit types and discuss some evolutionary implications. TOMATO AGAMOUS-LIKE 1 (TAGL1) is a MADS-box transcription factor gene that belongs to the PLENA (PLE) lineage within the AGAMOUS (AG) clade. The most well-studied genes in this lineage are the SHATTERPROOF (SHP) genes in Arabidopsis, known to be involved in dehiscence zone formation during silique development. In tomato, TAGL1 has been shown to control several aspects of tomato fruit ripening. Most notably, carotenoid synthesis seems to be controlled by TAGL1, likely via the ethylene synthesis and signaling pathway and in combination with RIPENING INHIBITOR (RIN). In addition, TAGL1 regulates genes involved in cell cycle regulation, flavonoid and lignin biosynthesis, and cuticle development. We discuss many of the genes in these different pathways that are likely controlled by TAGL1, directly or indirectly. We also examine the relationship of TAGL1 with known and putative interaction partners. PLE lineage genes have also been examined in other species such as Antirrhinum, Petunia, and Nicotiana and provide an interesting example of conservation and diversification of function in species that produce very different types of fleshy and dry fruits. The control of lignification may be a common mechanism for this group of genes. Lastly, we discuss future work needed to elucidate the TAGL1 regulatory pathway in tomato and to help better understand the functional diversification of genes in this lineage in related species.